title: HCT116_sicontrol_48h_batch2_rep2

source_name: HCT116 p53-/- transfected with siCtrl 48h


title: HCT116_siBYSL_48h_batch2_rep1

status: Public on Mar 09 2025

type: RNA

city: Salamanca

first: L. Francisco

last: Lorenzo-Martín

state: Castilla y León

address: Campus Miguel de Unamuno

country: Spain

institute: University of Salamanca (USAL) – Centro de Investigación del Cáncer (CIC)

description: Microarray data from HCT116 p53-/- transfected with siBYSL 48h

hyb_protocol: Clariom S arrays (Affymetrix) under standard conditions

scan_protocol: Standard Affymetrix protocol

data_processing: Upon microarray hybridization, the raw data was normalized with RMA using R (v.3.6.3)

label: biotin

molecule: total RNA

organism: Homo sapiens

label_protocol: Total RNA was quantified using 6000 Nano Chips (Agilent Technologies, Santa Clara, CA) and used (2.3 mg/sample) to generated labeled cRNA probes according to the manufacturer's instructions (Affymetrix)

growth_protocol: Cells were were cultured with Dulbecco´s modified Eagle´s medium supplemented with 10% fetal bovine serum, 100 U/ml penicillin, 2 mM L-glutamine (Gibco), and mainteined at 37 ºC wit 5 % CO2.

extract_protocol: Total RNAs were extracted using the Rneasy Mini Kit (Cat. #74104, Qiagen)

treatment_protocol: Cells were reverse transfected with 10 nM of the siRNA duplexes (listed in Supplementary Table 2 of manuscript) using 8 uL/mL of Lipofectamine RNAiMAX (Cat No. 13778150, Life Technologies) .  Cells were harvested at 24 h  48 h post-transfection. Negative controls were transfected with a control scrambled siRNA (Ambion / #4390843).

tag: cell line

value: HCT116 human colon cancer

tag: p53 status

tag: transfection

value: siBYSL

tag: time


title: HCT116_sicontrol_24h_batch2_rep3

description: Microarray data from HCT116 p53-/- transfected with siCtrl 24h

value: siCtrl


title: HCT116_sicontrol_48h_batch2_rep3

description: Microarray data from HCT116 p53-/- transfected with siCtrl 48h


title: HCT116_sicontrol_24h_batch2_rep2

source_name: HCT116 p53-/- transfected with siCtrl 24h


title: PP1γ KO 4sUDRB-seq 10min rep1

type: SRA

city: Shanghai

first: Aiwei

last: Wu

state: Shanghai

address: 800 Dongchuan Road

department: School of Life Sciences and Biotechnology

country: China

institute: Shanghai Jiao Tong University

library_source: transcriptomic

data_processing:
Library strategy: 4sU-DRB-seq
Raw reads were trimmed for adaptor sequence and low-complexity or low-quality sequence were filtered using fastp v0.13.1 with default parameters. ChIP-seq, ATAC-seq, CUT&Tag and 4sUDRB-seq reads were aligned using bowtie2 v2.3.4.1. RNA-seq read were aligned using hisat2 v2.1.0 with parameter --rna-strandness RF. TT-seq reads were aligned using STAR v2.7.9a.
For ChIP-seq, ATAC-seq, CUT&Tag and 4sUDRB-seq, normalized aboundency in each 50-bp genomic bin were generated into bigWig file using deepTools bamCoverage command with parameter --normalizeUsing CPM. For RNA-seq, read/fragment count were obtained using featureCounts v1.6.1. To create scaled, strand-specific BigWig files for TT-seq data, SAMtools was used to split each target BAM file into mappable reads to either forward or reverse strand, and bamCoverage function of deepTools was used with the “-scaleFactor” argument to convert each strand-specific BAM file to a scaled BigWig file.
Genome_build: Hg38 for RNA-seq, ChIP-seq, ATAC-seq, and CUT&Tag read mapping, hg19 for 4sUDRB-seq. Hg38 and sacCer3 for TT-seq read mapping.
Supplementary_files_format_and_content: Tab-delimited text files include fragment count values of genes for each sample.
Supplementary_files_format_and_content: BigWig files contains normalized read count (CPM, in 50-bp bins) for ChIP-seq, CUT&Tag and 4sUDRB-seq, normalized insertion count (CPM, in 50-bp bins) for ATAC-seq, normalized read count (by sequencing read aligned to yeast genome, in 50-bp bins) for TT-seq.

source_name: K562 cell line

growth_protocol: Human cells K562 were cultured in 90% DMEM + 10% FBS+ 2% Penicillin/Streptomycin + 2 mM L-Glutamine.

extract_protocol:
After RNA biotinylation and free biotin removal, biotinylated RNA was purified by streptavidin-coupled Dynabeads (Thermo, cat. no. 11205D). rRNA was depleted before library construction. Sequencing libraries were constructed by following the Illumina TruSeq RNA Library preparation protocol.
Sequencing libraries were constructed by following the Illumina TruSeq RNA Library preparation protocol.

treatment_protocol: 10 million cells were used for each experiment. After DRB (Sigma, cat. no. D1916) treatment and 4sU (Sigma, cat. no. T4509) incorporation, total RNA was extracted.

tag: treatment

value: PP1gamma knockout


title: HCT116_siRPS19-1_24h_batch2_rep1

description: Microarray data from HCT116 p53-/- transfected with siRPS19 24h


title: HCT116_siRPS19-1_24h_batch2_rep2

source_name: HCT116 p53-/- transfected with siRPS19 24h


title: rbohE 4h - 2

city: Seoul

first: Yuree

last: Lee

address: 1 Gwanak-ro, Gwanak-gu

department: Biological Sciences

country: South Korea

institute: Seoul National University

data_processing:
The TruSeq universal and indexed adapters were trimmed using Trimmomatic software v0.39 (Bolger et al. 2014). The reads obtained from sequencing were filtered using PRINSEQ v0.20.4 (Schmieder et al., 2011).
The filtered reads were aligned to the TAIR10.1 reference genome (GCF_000001735.4) using HISAT2 v2.2.1 (Kim et al., 2019). The resulting gene-level expression data were compiled into a matrix representing expression levels in transcripts per million (TPM). To account for compositional biases across samples, we applied trimmed mean of M-values (TMM) normalization using the edgeR package v3.19 in R (Robinson et al., 2010).
Assembly: Gene abundances were quantified with StringTie v1.3.4 (Pertea et al., 2015), utilizing the TAIR10 genome GFF3 annotation file (https://www.arabidopsis.org/download/file?path=Genes/TAIR10_genome_release/TAIR10_gff3/TAIR10_GFF3_genes.gff) for reference gene identification
Supplementary files format and content: raw count value in gene_count_matrix.csv

organism: Arabidopsis thaliana

source_name: leaves

growth_protocol: We used 8th leaf of 4-week-old Arabidopsis thaliana for mechanical wounds

extract_protocol:
RNA was extracted using RNeasy Plant Mini kit (Qiagen) following the manufacturer's protocol. 1ug of RNA were used to generate library.
mRNA-sequencing libraries were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) according to the provided protocol

treatment_protocol: Mechanical wounds were given to leaf with stainless-steel blades (size 10, FEATHER) attached to a laboratory knife

tag: tissue

value: leaves

tag: genotype

value: rbohE

value: wound


title: control (for PP1γ KO) 4sUDRB-seq 0min rep2

value: control


title: HCT116_siRPS19-1_24h_batch2_rep3


title: PP1γ KO 4sUDRB-seq 0min rep1


title: rbohE 4h - 1


title: control (for PP1γ KO) 4sUDRB-seq 10min rep1


title: PP1γ KO ser5p CUT&Tag rep2

library_source: genomic

data_processing:
Library strategy: CUT&Tag
Raw reads were trimmed for adaptor sequence and low-complexity or low-quality sequence were filtered using fastp v0.13.1 with default parameters. ChIP-seq, ATAC-seq, CUT&Tag and 4sUDRB-seq reads were aligned using bowtie2 v2.3.4.1. RNA-seq read were aligned using hisat2 v2.1.0 with parameter --rna-strandness RF. TT-seq reads were aligned using STAR v2.7.9a.
For ChIP-seq, ATAC-seq, CUT&Tag and 4sUDRB-seq, normalized aboundency in each 50-bp genomic bin were generated into bigWig file using deepTools bamCoverage command with parameter --normalizeUsing CPM. For RNA-seq, read/fragment count were obtained using featureCounts v1.6.1. To create scaled, strand-specific BigWig files for TT-seq data, SAMtools was used to split each target BAM file into mappable reads to either forward or reverse strand, and bamCoverage function of deepTools was used with the “-scaleFactor” argument to convert each strand-specific BAM file to a scaled BigWig file.
Genome_build: Hg38 for RNA-seq, ChIP-seq, ATAC-seq, and CUT&Tag read mapping, hg19 for 4sUDRB-seq. Hg38 and sacCer3 for TT-seq read mapping.
Supplementary_files_format_and_content: Tab-delimited text files include fragment count values of genes for each sample.
Supplementary_files_format_and_content: BigWig files contains normalized read count (CPM, in 50-bp bins) for ChIP-seq, CUT&Tag and 4sUDRB-seq, normalized insertion count (CPM, in 50-bp bins) for ATAC-seq, normalized read count (by sequencing read aligned to yeast genome, in 50-bp bins) for TT-seq.

molecule: genomic DNA

extract_protocol:
CUT&Tag experiments were performed as previously described with minor modifications(Kaya-Okur, H.S. et al. ). Briefly, 100,000 cells were used for each experiment. Cells were bound to Concanavalin A-coated beads without fixation and chromatin opening. After primary and secondary antibodies binding, pA-Tn5 transposome binding, and tagmentation, DNA was extracted by PCI.
Library was construct by PCR, NEB Phusion(M0530L) was used as protocol.

tag: antibody

value: Pol II (ser5p)


title: control (for PP1γ KO) 4sUDRB-seq 0min rep1


title: PP1γ KO ser5p CUT&Tag rep1


title: rbohE 1D - 1


title: rbohE 2h - 3


title: HCT116_sicontrol_24h_batch2_rep1


title: HCT116_siBYSL_48h_batch1_rep3

source_name: HCT116 p53-/- transfected with siBYSL 48h


title: control (for PP1γ KO) ser5p CUT&Tag rep1


title: control (for PP1γ KO) ser5p CUT&Tag rep2


title: PP1γ KO ser2p CUT&Tag rep2

value: Pol II (ser2p)


title: HCT116_siBYSL_48h_batch1_rep2


title: PP1γ KO ser2p CUT&Tag rep1


title: rbohE 2h - 1


title: control (for PP1γ KO) ser2p CUT&Tag rep1


title: rbohE 0h - 3


title: PP1γ KO Pol II CUT&Tag rep1

value: Pol II


title: control (for PP1γ KO) ser2p CUT&Tag rep2


title: control (for PP1γ KO) Pol II CUT&Tag rep2


title: control (for PP1γ KO) Pol II CUT&Tag rep1


title: HCT116_sicontrol_48h_batch1_rep3


title: rbohE 0h - 2


title: HCT116_siBYSL_48h_batch1_rep1


title: anti-MED1_Y125A-NS1-A549_CUTandRUN_rep1

city: St. Louis

first: Jacqueline

last: Payton

state: MO

address: 660 S. Euclid Ave

department: Pathology and Immunology

country: USA

institute: Washington University School of Medicine

description: Library name: anti-MED1_Y125A-NS1-A549_CUTandRUN_rep1

data_processing:
CUT&RUN fastq files were aligned and analyzed using a pipeline adapted from (PMID: 31232687,31300027,34461601,34644581) using bowtie2, samtools, SEACR, and bedtools with default parameters.
Assembly: GRCh38.101
Supplementary files format and content: There is a .bed file for each sample that contains called peaks with their chromosomes, start and end positions.
Library strategy: CUT&RUN

growth_protocol: A549 cells were maintained in F12K media.

extract_protocol:
Nuclei were harvested per Epycypher CUT&RUN CUTANA protocol.
Libraries were constructed per Epycypher CUT&RUN CUTANA protocol.
CUT&RUN dual indexing and library preparation using Epycypher CUT&RUN CUTANA library preparation kit.

treatment_protocol: A549 cells were transfected with empty vector (EV), wild type (WT), or mutant (E110A, E110A/L132A/L133A [ELL], Y125A) NS1 constructs and nuclei harvested 24 hours later.

tag: cell type

value: Epithelial


title: rbohE 0h - 1


title: HCT116_sicontrol_48h_batch1_rep2


title: HCT116_sicontrol_48h_batch1_rep1


title: rbohE 2h - 2


title: gpat4 gpat8 3D - 2

value: gpat4 gpat8


title: anti-MED1_WT-NS1-A549_CUTandRUN_rep2

description: Library name: anti-MED1_WT-NS1-A549_CUTandRUN_rep2


title: HCT116_siRPS19-1_24h_batch1_rep3


title: HCT116_siRPS19-1_24h_batch1_rep2


title: gpat4 gpat8 3D - 1


title: anti-MED1_EV-A549_CUTandRUN_rep2

description: Library name: anti-MED1_EV-A549_CUTandRUN_rep2


title: HCT116_sicontrol_24h_batch1_rep3


title: PP1γ KO Pol II CUT&Tag rep2


title: HiC, D907, Non-assimilated

city: Barcelona

first: Gonzalo

last: Sabarís

address: Passeig del Migdia s/n

institute: IBB-CSIC

data_processing:
Hi-C samples were analyzed using the TADbit pipeline
Paired-end reads were mapped to the D. melanogaster dm6 reference genome using Bowtie 2 (v.2.3.5.1), taking into account the restriction enzyme DpnII religation sites
All valid pair interactions were then processed using the Cooler package (v. 0.9.1) to generate .cool files at 100bp resolution. .mcool files at different resolutions (100bp to 40Kb) were obtained and normalized using the Iterative Correction and Eigenvector Decomposition algorithm (ICE) with default parameters.
Assembly: dm6
Supplementary files format and content: mcool files of each library for Hi-C matrix visualization

organism: Drosophila melanogaster

source_name: Wing imaginal disc

growth_protocol: Flies were raised at 25°C in standard medium

extract_protocol:
HiC was performed according the protocol of the EpiTect Hi-C Kit from Qiagen
Libraries were prepared according the protocol of the EpiTect Hi-C Kit from Qiagen

tag: Sex

value: female

tag: developmental stage

value: Wing imaginal disc

tag: group

value: Non-assimilated


title: anti-MED1_EV-A549_CUTandRUN_rep1

description: Library name: anti-MED1_EV-A549_CUTandRUN_rep1


title: gpat4 gpat8 3D - 3


title: HiC, D907, Control

value: Control


title: anti-MED1_Y125A-NS1-A549_CUTandRUN_rep2

description: Library name: anti-MED1_Y125A-NS1-A549_CUTandRUN_rep2


description: Library name: anti-MED1_E110A/L132A/L133A-NS1-A549_CUTandRUN_rep2


title: anti-MED1_WT-NS1-A549_CUTandRUN_rep1

description: Library name: anti-MED1_WT-NS1-A549_CUTandRUN_rep1


title: gpat4 gpat8 2D - 3


title: gpat4 gpat8 2D - 1


description: Library name: anti-MED1_E110A/L132A/L133A-NS1-A549_CUTandRUN_rep1


title: gpat4 gpat8 2D - 2


title: HiC, D907, Assimilated

value: Assimilated


title: anti-MED1_E110A-NS1-A549_CUTandRUN_rep2

description: Library name: anti-MED1_E110A-NS1-A549_CUTandRUN_rep2


city: Evry

first: Marco Antonio

last: MENDOZA-PARRA

address: 2 rue Gaston Crémieux

country: France

institute: CNRS UMR8030

description: Library name: Control_FFPE_decalcified_MousePaws_H3K27ac_CMM_DV

data_processing:
Primary analysis has been performed with our in-house developped tool SysISTD (SysFate Illumina Spatial transcriptomics Demultiplexer: https://github.com/SysFate/SysISTD). SysISTD takes as entry paired-end sequenced reads (fastq or fastq.gz format), and two TSV files, the first one containing the sequence of the molecular barcodes associated to the rows or column in the printed arrays and the second file presenting the physical position architecture of the spatial barcodes. SysISTD search for the Gibson sequence (regex query), then for two neighboring barcodes. Paired-reads presenting these features were aligned to either the human (hg19) or the mouse (mm9) genome with Bowtie2. In this manner, SysISTD generates SAM files per spatial coordinate, which are reprocessed with the help of featureCounts 31 and an in-house script for detecting reads counts per gene promoter (+/-5kb) and per spatial coordinate (SysISPD (SysFate Illumina Spatial Promoters Demultiplexer: https://github.com/SysFate/SysISPD). As outcome, SysISPD generates a matrix presenting read counts associated to physical coordinates in columns and known transcripts ID associated to the evaluated promoters in rows. To focus the downstream analysis to the physical positions corresponding to the analyzed tissue, we used an in-house R script taking as entry an image of the DNA array scanned with the TRICT filter, releaving the presence of the fiducial borders. Specifically, we upload to R a croped image within the fiducials (imager package) and we use the “px.flood” function to retrieve the pixels associated to the tissue. Finally we applied a pixel to gexel coordinates conversion prior to cross this information with the outcome of SysISPD. In parallel, all SAM files per coordinate were merged into a single-file and processed with the peak caller Macs2 5. The obtained confident peaks (P-value < 1x10-5) were annotated to their proximal coding regions. Finally, the spatial coordinates/promoters matrix provided by SysISPD was filtered by selecting transcripts associated to promoter regions presenting confident peaks. The “confident promoter peaks tissue-focused” matrix was quantile normalized with our previously described tool MULTILAYER 4,32. Then, the normalized matrix was further processed for revealing cell/tissue density contrast information by integrating normalized counts with pixel value levels retrieved in either the DAPI or the histone modification immunostaining image. The obtained normalized and pixels intensity adjusted matrix was used for all downstream analysis.
Assembly: mm9
Supplementary files format and content: Normalized spatial matrices

organism: Mus musculus

source_name: paws

extract_protocol:
Tissue samples were cryosectionned (16 µm sections for mouse embryo tissue and  and 20 µm sections for mouse brain WT tissue), collected between -15°C and -12°C then deposited on the DNA-arrays defined regions. Tissue sections were fixed with 1% Para-formaldheyde (PFA) (Life technologies 28908) in 1X PBS (Life technologies 70011-036) at room temperature during 30 minutes, then washed three times with 1X PBS. for FFPE samples, Mouse paws have been collected and fixed in 4% Paraformaldehyde (PFA) in 1X PBS for 24 hours. Then paws have been decalcified for 7 consecutive days in  an +4°C refrigerated room on agitation. A maximum of 5 paws, each inside a cassette, are decalcified  in 1L solution of 8,5g NaCl, 10% formol and 100mL of 99-100% glacial acetic acid. At mid incubation time, decalcification solution has been renewed and paws have been cut in half and put back into the cassette. Once decalcification is finished, paws have been rinsed in ddH20 to then be dehydrated and infiltrated with paraffin using an automated device (Novalix facilities) following this standard protocol: 70% ethanol, 2 washes of 70% ethanol, 96% ethanol, 100% ethanol, half and half mix of 100% ethanol with 100% xylene, empty the incubation chamber and final step of 100% xylene. Once dehydrated, each half paw has been paraffine embedded. All blocks of mouse paw FFPE can be stored at room temperature. 4µM thick sections around the Talus bone have been collected from those FFPE mouse paw blocks using a microtome. Paw FFPE sections were deparaffined and rehydrated following standard protocols on an automated device (Novalix facilities). Tissue sections were permeabilized during 1 hour at room temperature with 1% Triton X-100 (Merck 93443) in 1X PBS solution, then washed three times with 1X PBS followed by 5 minutes incubation at room temperature with 0.1M HCl for chromatin loosening, and washed three times with 1X PBS. Afterwads, sections were covered during 1 hour at room temperature with a blocking and permeabilizing mix (10% Normal Donkey serum (NDS) (Merck D9663), 0.25% Triton X-100 (Merck 93443), 0.1% Bovine Serum Albumin (BSA) (Merck A9418), 2 mM EDTA (Life technologies AM9260G), 0.5 mM Spermidine (Life technologies 132740010), 1 tablet EDTA-free Protease Inibitor Cocktail (PIC) (Merck 11873580001)) prepared in 1X PBS. Then tissue were washed twice with 150 mM NaCl Buffer (20 mM Hepes-KOH 1M pH=7.2 (Merck 391338), 150 mM NaCl; 0.1 % BSA, 0.5 mM Spermidine, 1 tablet PIC). After that, sections were incubated with a solution containing 2 μg of a selected primary Antibody (anti-Histone H3 (tri methyl K4) antibody (Abcam ab213244); anti-Histone H3 (acethyl K27) antibody (Abcam ab177178); or anti-Histone H3 (tri methyl K27) antibody (Abcam ab 192985)), in 150 mM NaCl Buffer at room temperature during 1 hour, followed by washing twice with 150 mM NaCl Buffer. Then tissues were incubated at room temperature with a pre-incubated mix (2 hours) containing 1:1 molar ratio of unloaded fusion protein pA-Tn5 (in-house production (9.5 µM) prepared using the plasmid Addgene #124601 by following previous protocols 3,30, and the corresponding secondary Antibody (donkey anti-rabbit IgG antibody (Merck SAB3700932). The pre-incubated mix was complemented with either a conjugated Alexa-488 secondary Antibody (donkey anti-rabbit IgG antibody, Alexa Fluor 488 (Life technologies A-21206)) or a conjugated Alexa-555 secondary Antibody (donkey anti-rabbit IgG antibody, Alexa Fluor 555 (Abcam ab 150062)), added at 1/1,000 dilution. The pre-incubated mix was used in a 5 times molar excess relative to the number of moles of the MOS sequence deposited per DNA array: for 2,048 spots it corresponds to 2,56.10-12 moles; for 4,096 spots it corresponds to 5,12.10-12 moles. After 3 hours of incubation with the assembled mix, tissue sections were washed twice with 300 mM NaCl Buffer (20 mM Hepes-KOH 1M pH=7.2, 300 mM NaCl; 0.1 % BSA, 0.5 mM Spermidine, 1 tablet PIC) to remove PA-Tn5-antibodies in excess. Then, tissue sections were incubated during 5 minutes with DAPI (Life technologies 62248) 1/5,000 dilution in 1xPBS, then washed twice with PBS 1X. DNA arrays were scanned under the TRICT filter to reveal the presence of the fiducial borders as well as the physical position of tissue sections on top of the printed DNA arrays. In case of the use of the conjugated Alexa-488 secondary Antibody, an additionnal scanning using FITC filter was done to reveal the localisation of the studied modification of histone. Finally, an additional scanning of the tissue using DAPI filter was done to observe nuclear localisation and to get cell density information. After imaging, sections were incubated overnight at 37°C with 10 mM MgCl2 (Life technologies AM9530G) in 300 mM NaCl Buffer for in situ tagmentation. Next day, tissue sections were incubated with a mix containing 2.25 µL of EDTA 0.5 M, 2.75 µL of 10% Sodium dodecyl sulfate (SDS) (Merck 71736) and 0.5 µL of 20 mg/mL Proteinase K (Life technologies 25530049) in 50 µL of ddH2O overnight at 37 °C. The mix volume was ajusted depending of the slide printing area. Finally, slides were washed under agitation (300 rpm) in containers containing: 100 mL of preheated buffer 1 (2X SSC and 0.1% SDS) during 15 minutes at 50°C, then 10 minutes with buffer 2 (0.2X SSC) at room temperature and 10 minutres with buffer 3 (0.1X SSC) at room temperature. At last the slides were washed in ddH2O and spin-dried. After that, DNA arrays were incubated with 0.1 M NaOH solution during 10 minutes at room temperature and then neutralized after removing liquid with a mix containig 100 µL of 0.1 M NaOH, 11.8 µL of 10X TE and 6.5 µL of 1.25 M acetic acid during 2-3 minutes. Finally, slides were washed within a falcon containing buffer 3 (0.1X SSC), then into a falcon containing ddH2O and spin-dried.
DNA arrays were incubated with a poly-C tailing mix containing 0.6 U/µL terminal transferase (New England Biolabs M0315), 1X terminal transferase buffer (New England Biolabs BO315), 0.3X CoCl2 (New England Biolabs B0252), 0.2 mM dCTP and 0.01 mM ddCTP during 35 minutes at 37°C, then 20 minutes at 70°C, and cooled at 12°C. To avoid evaporation, each printing area was closed by using sealing chambers (Life technologies AB-0576 (size 25 μL) or AB-0577 (size 65 μL)). Finally, slides were washed within a falcon containing buffer 3, and a falcon containing ddH2O, prior spin-dry. DNA arrays were then incubated with a mix containing:  0.1 U/µL  Klenow exo- (New England Biolabs M0212), 0.2 mg/mL BSA, 1X NEB2 buffer (New England Biolabs B7002), 0.5 mM dNTPs and an oligonucleotide (1 µM) having a complementary sequence for the poly-C tailed sequence, as well as a 5’-extremity providing an adapter sequence (“GTTCAGACGTGTGCTCTTCCGATCTGGGGGGGGGH”). As in the preceding step to avoid evaporation, the reaction was performed by using sealing chambers.The following conditions were used: 47°C during 5 minutes (primer annealing), 37°C during 1 hour (extension), 10 minutes at 70°C (enzyme deactivation) and cooled et 12°C. In order to recover the synthesized complementary DNA strands, DNA arrays were incubated with 100 µL of 0.1 M NaOH solution during 10 minutes, then the liquid was collected and a second incubation with 0.1 M NaOH solution was repetead. The two fractions were collected together and neutralized (for 100 µL 0.1 M NaOH solution add 11.8 µL of 10X TE and 6.5 µL of 1.25 M acetic acid). DNA arrays were also neutralized with 100 µL 0.1 M NaOH solution mixed with 11.8 µL of 10X TE and 6.5µL of 1.25 M acetic acid, then washed with buffer 3 and ddH2O and stored at 4 °C  for eventual supplementary experiments. The neutralized collected solution were precipitated with cold 100% ethanol (2X volume),  200 mM NaCl and 1 µL of 20 mg/mL glycogen (Merck 10901393001) and conserved at -20°C for at least 1 hour, then centrifuged at 12,500 rpm during 30 minutes, washed once with cold 70% ethanol and resuspended (after 30 minutes air drying) in 23 µL of ddH2O. Half of the resuspended cDNA’s complementary-probe material (12ul) was adjusted to 23ul with water, then mixed with: 25 µL of Q5 hot start high fidelity 2X master mix (New England Biolabs M0464L), 1 µL of adapter seq1 primer (0.02 µM (GTTCAGACGTGTGCTCTTCCGATCT)) and 1 µL of adapter seq2 primer (0.02 µM, (TACACTCTTTCCCTACACGACGCTCTTCCGATCTGACTCGTAATAC, corresponding to a part of the T7-promoter sequence)). This mix was amplifyied by PCR  (98°C, 30 s; 15 cycles: 98°C 10 s; 65°C 75 s; then 65°C 5 min; 12°C hold) and then cleanned at 0.9X ratio with SPRIselect beads (Beckman Coulter B23318) and resuspended in 24 µL of ddH2O. A second PCR amplification was done by mixing the 24 µL of adaptors linked cDNA’s complementary-probe strand with: 25 µL of Q5 hot start high fidelity 2X master mix, 0.5 µL of universal primer and 0.5 µL of index primer (0.1 µM final concentration NEBNext multiplex oligos for Illumina index primers set 1 E7335) by using the following cycling conditions: 98°C for 30 s, 15 cycles of 98°C for 10 s and 65°C for 75 s; then final extension at 65°C for 5 min and hold at 12°C. Then sample was cleanned at 0.9X ratio with SPRIselect beads. At last the library was used for Illumina sequencing (150 nts paired-ends sequencing; NovaSeq).

value: paws

value: Adult

tag: disease state

value: 42 days


title: HCT116_sicontrol_24h_batch1_rep2

value: HCT116 human colon cancer

value: siCtrl


title: gpat4 gpat8 1D - 3

value: leaves

value: wound


title: HCT116_siRPS19-1_24h_batch1_rep1


title: HCT116_sicontrol_24h_batch1_rep1


title: WT mice, cerebellum, rep3

city: Kunming

first: Ming

last: Li

state: Yunnan

address: NO 32 Jiao-Chang Donglu

institute: Kunming Institute of Zoology, Chinese Academy of Sciences

data_processing:
Illumina NovaSeq 6000 reads were trimmed and clipped for quality control in Trimmomatic v0.39. (parameter: LEADING:3 TRAILING:3 SLIDINGWINDOW:4:15 HEADCROP:15)
High-quality reads were then imported into STAR 2.7.10 for alignment into BAM files. (parameter: --genomeDir /path/to/ensembl_mm10/ --outSAMtype BAM SortedByCoordinate)
Gene read counts were calculated by featureCounts v2.0.3. (parameter: -T 24 -s 0 --primary -p -t exon -Q 10 -g gene_id -a /path/to/Mus_musculus.GRCm38.94.chr.gtf)
Assembly: ensembl_mm10
Supplementary files format and content: whitespace-delimited text file including raw counts for each hippocampus Sample
Supplementary files format and content: whitespace-delimited text file including raw counts for each cerebelllum Sample

source_name: Cerebellum

extract_protocol:
Total RNA was extracted by Trizol.
RNA library for Strand-specific RNA-seq was prepared as fllows: mRNA was purified  from total RNA using polyT and then fragmented into 300~350 bp fragments, the first strand cDNA was reverse-transcribed using fragmented RNA and dNTPs (dATP, dTTP, dCTP and dGTP) and second strand cDNA was synthesised using DNA polymerase I and dNTPs (dATP, dUTP, dCTP and dGTP). Remaining overhangs of double-strand cDNA were converted into blunt ends via exonuclease/polymerase activities. After adenylation of 3’ ends of DNA fragments, sequencing adaptors were ligated to the cDNA and the library fragments were purified. The template without U was enriched by PCR, and the PCR product was purified to obtain the final library.

value: Cerebellum

value: WT


title: gpat4 gpat8 1D - 2


title: WT mice, cerebellum, rep2


title: HCT116_p53_siBYSL_48h_batch2_rep3

description: Microarray data from HCT116 p53+/+ transfected with siBYSL 48h

value: siBYSL


title: anti-HA_Y125A-NS1-A549_CUTandRUN_rep2

description: Library name: anti-HA_Y125A-NS1-A549_CUTandRUN_rep2

value: Epithelial


title: HCT116_p53_siBYSL_48h_batch2_rep1

source_name: HCT116 p53+/+ transfected with siBYSL 48h


title: WT mice, cerebellum, rep1


title: gpat4 gpat8 1D - 1

value: gpat4 gpat8


title: anti-HA_Y125A-NS1-A549_CUTandRUN_rep1

description: Library name: anti-HA_Y125A-NS1-A549_CUTandRUN_rep1


title: HCT116_p53_siBYSL_48h_batch2_rep2


title: HCT116_p53_sicontrol_48h_batch2_rep3

description: Microarray data from HCT116 p53+/+ transfected with siCtrl 48h


title: gpat4 gpat8 12h - 3


title: SYT14-KO mice, cerebellum, rep3

value: SYT14 knockout


title: gpat4 gpat8 12h - 2


title: HCT116_p53_sicontrol_48h_batch2_rep2

source_name: HCT116 p53+/+ transfected with siCtrl 48h


title: anti-HA_WT-NS1-A549_CUTandRUN_rep2

description: Library name: anti-HA_WT-NS1-A549_CUTandRUN_rep2


title: SYT14-KO mice, cerebellum, rep2


title: HCT116_p53_sicontrol_48h_batch2_rep1


title: gpat4 gpat8 12h - 1


title: anti-MED1_E110A-NS1-A549_CUTandRUN_rep1

description: Library name: anti-MED1_E110A-NS1-A549_CUTandRUN_rep1


title: WT mice, hippocampus, rep3

source_name: Hippocampus

value: Hippocampus


title: gpat4 gpat8 4h - 3


title: anti-HA_EV-A549_CUTandRUN_rep2

description: Library name: anti-HA_EV-A549_CUTandRUN_rep2


title: anti-HA_WT-NS1-A549_CUTandRUN_rep1

description: Library name: anti-HA_WT-NS1-A549_CUTandRUN_rep1


title: SYT14-KO mice, cerebellum, rep1


title: anti-HA_EV-A549_CUTandRUN_rep1

description: Library name: anti-HA_EV-A549_CUTandRUN_rep1


title: HCT116_p53_siRPS19_24h_batch2_rep3

description: Microarray data from HCT116 p53+/+ transfected with siRPS19 24h


title: WT mice, hippocampus, rep2


title: gpat4 gpat8 4h - 2


description: Library name: anti-HA_E110A/L132A/L133A-NS1-A549_CUTandRUN_rep2


title: WT mice, hippocampus, rep1


description: Library name: anti-HA_E110A/L132A/L133A-NS1-A549_CUTandRUN_rep1


title: gpat4 gpat8 4h - 1


title: gpat4 gpat8 2h - 3


title: SYT14-KO mice, hippocampus, rep3


title: anti-HA_E110A-NS1-A549_CUTandRUN_rep2

description: Library name: anti-HA_E110A-NS1-A549_CUTandRUN_rep2


title: gpat4 gpat8 2h - 1


title: SYT14-KO mice, hippocampus, rep2


title: gpat4 gpat8 2h - 2


title: anti-HA_E110A-NS1-A549_CUTandRUN_rep1

description: Library name: anti-HA_E110A-NS1-A549_CUTandRUN_rep1


title: SYT14-KO mice, hippocampus, rep1


title: HCT116_p53_siRPS19_24h_batch2_rep2

source_name: HCT116 p53+/+ transfected with siRPS19 24h


title: gpat4 gpat8 0h - 2


title: anti-ATF3_Y125A-NS1-A549_CUTandRUN_rep2

description: Library name: anti-ATF3_Y125A-NS1-A549_CUTandRUN_rep2


title: anti-ATF3_Y125A-NS1-A549_CUTandRUN_rep1

description: Library name: anti-ATF3_Y125A-NS1-A549_CUTandRUN_rep1


title: HCT116_p53_sicontrol_24h_batch2_rep1

description: Microarray data from HCT116 p53+/+ transfected with siCtrl 24h


title: HCT116_p53_siBYSL_48h_batch1_rep3

description: Microarray data from HCT116 p53+/+ transfected with siBYSL 48h


title: HCT116_p53_sicontrol_24h_batch2_rep2

source_name: HCT116 p53+/+ transfected with siCtrl 24h


title: HCT116_p53_sicontrol_24h_batch2_rep3


title: HCT116_p53_siBYSL_48h_batch1_rep2


title: gpat4 gpat8 0h - 1


title: anti-ATF3_WT-NS1-A549_CUTandRUN_rep1

description: Library name: anti-ATF3_WT-NS1-A549_CUTandRUN_rep1


title: HCT116_p53_siRPS19_24h_batch2_rep1


title: HCT116_p53_siBYSL_48h_batch1_rep1


title: Tyr_3

city: Pamplona

first: Ana

last: Garcia Osta

state: Navarra

address: Juslarrocha 10, 3H

department: neuroscience

institute: Cima

data_processing:
The quality of the samples was verified using FastQC v0.11.8 software.
FASTQ files were mapped to GRCm39 human assembly using STAR v2.7.9a with default parameters.
featureCounts v1.6.0 software and Gencode M30 release were used to obtain read counts at gene level considering only those genes aligned to exonic regions
Obtained gene counts were normalized using edgeR and voom function of limma
Assembly: GRCm39
Supplementary files format and content: tab-delimited text files include gene counts obtained with featureCounts for each sample.

molecule: polyA RNA

source_name: midbrain

extract_protocol:
RNA was isolated from mice midbrain using Maxwell RSC simplyRNA Tissue Kit (Promega). The integrity of the RNA was subjected to quantity and quality control using Qubit HS RNA Assay Kit (Thermo Fisher Scientific) and 4200 Tapestation with High Sensitivity RNA ScreenTape (Agilent Technologies). All RNA samples were high-quality, with RIN values higher than 7.
Library preparation was performed using the Illumina Stranded mRNA Prep Ligation kit (Illumina) following the manufacturer’s protocol. All sequencing libraries were constructed from 100 ng of total RNA according to the manufacturer's instruction.

treatment_protocol: Mice were injected in the substantia nigra with 1 ul of AAV-hTyr ir AAV control and sacrificed 5 weeks after injection. Midbrain was extracted to obtain RNA

value: midbrain

value: Snca-;PAC-Tg -SNCAWT

value: AAV-hTyr


title: HCT116_p53_sicontrol_48h_batch1_rep2


title: anti-ATF3_EV-A549_CUTandRUN_rep2

description: Library name: anti-ATF3_EV-A549_CUTandRUN_rep2


title: Tyr_2


title: gpat4 gpat8 0h - 3


title: Tyr_1


description: Library name: anti-ATF3_E110A/L132A/L133A-NS1-A549_CUTandRUN_rep2


title: C_3

value: AAV-polyA


title: C_2


title: anti-ATF3_WT-NS1-A549_CUTandRUN_rep2

description: Library name: anti-ATF3_WT-NS1-A549_CUTandRUN_rep2


title: anti-ATF3_E110A-NS1-A549_CUTandRUN_rep2

description: Library name: anti-ATF3_E110A-NS1-A549_CUTandRUN_rep2


title: exosome at Con, biological rep2

city: Beijing

first: Hua

last: Wang

address: 38 Xueyuan Road

department: Department of Pathology

institute: Peking University Health Science Center

description: miRNA expression data from mesenchymal stem cell derived exosome

hyb_protocol: Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Multispecies miRNA-4 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.

scan_protocol: GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000 7G.

data_processing: The data were analyzed with RMA using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.

source_name: hMSC  derived exosome at 48h

label_protocol: Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).

growth_protocol: Ewing sarcoma cells and mesenchymal stem cells were cultured in a humidified environment containing 5% CO2 at 37°C.

extract_protocol: Trizol extraction of total RNA was performed according to the manufacturer's instructions.

treatment_protocol: When the cell density rose to 70-80%, the culture media were replaced with serum-free DMEM. After 48 hours, the extracellular media were collected and exosomes of cells were extracted.

value: human mesenchymal stem cells

tag: exosome

value: mesenchymal stem cell derived exosome


title: C_1


title: HCT116_p53_sicontrol_48h_batch1_rep1

description: Microarray data from HCT116 p53+/+ transfected with siCtrl 48h

value: HCT116 human colon cancer


title: GSC MES20, stiff, shIQGAP3

status: Public on Mar 08 2025

city: Pittsburgh

first: Po

last: Zhang

state: Pennsylvania

address: 5115 Centre Ave

institute: University of Pittsburgh,

description: Library name: MES20 stiff shIQGAP3

data_processing:
Raw ChIP-seq reads were aligned to the human genome (hg38) using Bowtie2 (version 2.5.3) with default settings. Quality control was performed using FastQC (version 0.12.0), and adapter trimming was done with Trimmomatic (version 0.39). Read coverage was analyzed using deepTools (version 3.5.5) and visualized in the IGV software. Peak calling was performed with MACS2 (version 2.1.2) using default parameters and a p-value cutoff of 1 × 10-4. Heatmaps and metagene plots were generated using the computeMatrix function from deepTools.
Assembly: hg38
Supplementary files format and content: bigwig

source_name: glioblastoma

extract_protocol:
GSC cells were cross-linked with 1% paraformaldehyde for 10 minutes, followed by quenching with glycine for 5 minutes at room temperature. After cell lysis, the fixed chromatin was sonicated to generate DNA fragments ranging from 200 to 600 bp.
For library preparation, 1 to 10 ng of immunoprecipitated DNA and the NEBNext Ultra DNA Library Prep Kit for Illumina were used, and the samples were subjected to sequencing on the NovaSeq X Plus.

value: glioblastoma

value: GSC MES20

value: Human glioma stem cells


title: anti-ATF3_E110A-NS1-A549_CUTandRUN_rep1

description: Library name: anti-ATF3_E110A-NS1-A549_CUTandRUN_rep1


title: anti-ATF3_EV-A549_CUTandRUN_rep1

description: Library name: anti-ATF3_EV-A549_CUTandRUN_rep1


title: GSC3028, stiff, shIQGAP3

description: Library name: 3028 stiff shIQGAP3


title: GSC MES20, stiff, shCONT

description: Library name: MES20 stiff CONT

value: shCONT


title: exosome at T2, biological rep3

description: miRNA expression data from Ewing sarcoma derived exosome

source_name: SK-N-MC derived exosome at 48h

value: Ewing sarcoma cell line SK-N-MC

value: Ewing sarcoma derived exosome


title: GSC3028, stiff, shCONT

description: Library name: 3028 stiff CONT


title: HCT116_p53_siRPS19-1_24h_batch1_rep2

description: Microarray data from HCT116 p53+/+ transfected with siRPS19 24h


description: Library name: anti-ATF3_E110A/L132A/L133A-NS1-A549_CUTandRUN_rep1

value: Epithelial


title: exosome at T2, biological rep2


title: 77365_ST

city: Xiamen

first: Lin

last: Shuhai

state: Fujian

address: Room B205, Huang Zhaoyang Building, School of Life Sciences, Xiamen University

institute: Xiamen University

description:
Library name: 77365_breast_cancer_ST
Fresh frozen Spatial transcriptome sequencing

data_processing:
Raw sequencing data were processed using the bcl2fastq pipeline to generate FASTQ files.
Assembly: GRCh38
Supplementary files format and content: barcodes.tsv.gz: list of spatial barcodes
Supplementary files format and content: features.tsv.gz: list of gene lDs
Supplementary files format and content: matrix.mtx.gz:gene expresion count data in Matrix Market Exchange Format
Supplementary files format and content: scalefactors json.json:scalefactors in json format
Supplementary files format and content: tissue hires image.png: hi-res image of tissue
Supplementary files format and content: tissue lowres image.png: low-res image of tissue
Supplementary files format and content: tissue positions list.csv: list of spatial barcodes and the coordinates specifyingspots
Library strategy: Spatial Transcriptomics

source_name: breast cancer

extract_protocol:
Fresh Frozen tissue were OCT embedded, and 10um frozen sections were cut with cryostat
Spatial transcriptome sequencing libraries were prepared with DynaSpatial Spatial Gene Expression Slides & Reagent kit according to manufactuer's construction

value: breast cancer


title: HCT116_p53_siRPS19-1_24h_batch1_rep1


title: exosome at Con, biological rep3


title: 49129_ST

description:
Library name: 49129_breast_cancer_ST
Fresh frozen Spatial transcriptome sequencing


title: HCT116_p53_siRPS19-1_24h_batch1_rep3


title: 77137_ST

description:
Library name: 77137_breast_cancer_ST
Fresh frozen Spatial transcriptome sequencing


title: 43666_ST

description:
Library name: 43666_breast_cancer_ST
Fresh frozen Spatial transcriptome sequencing


title: 30215_ST

description:
Library name: 30215_breast_cancer_ST
Fresh frozen Spatial transcriptome sequencing


title: HUG9_HGNP591, Human sperm, 3NP GAM

first: Heming

last: Xu

address: Yiheyuan Road

institute: Peking University

data_processing:
Trim Galore (version 0.6.10) was used with default settings to automatically detect and remove adaptors. Low-quality and unmapped reads were filtered out using the view command from the samtools (version 1.17) package with options “-q 20 -F 4 -bS”. PCR duplicates were subsequently removed using the “markdup” command from samtools. Coverage across the genome was quantified in uniformly sized bins using the “multicov” command from bedtools (version 2.31.1), with bin sizes set at 50 Kb and 1 Mb. Positive bins were identified using the call_bins module from GAMtools (version 2.0.0) with a fixed threshold of 4. A normalized linkage disequilibrium (D') matrix was generated using the “matrix” function from GAMtools. The data were then converted into cool format using custom scripts. For the compartment analysis, we first corrected for distance effects in the 1 Mb D' matrix to generate an autocorrelation matrix. We then performed principal component analysis and selected the component with the highest correlation to GC content among the first three principal components same as ref2. TAD analysis was conducted using the same methods as bulk Hi-C.
Assembly: GRCh38
Supplementary files format and content: Tab-separated genome coverage for each sample.

source_name: Sperm cells

extract_protocol:
A human germ cell sample was used for Genome Architecture Mapping (GAM) experiments, cells were fixed by 0.1% formaldehyde for 10 minutes while rotating at room temperature. Then, 2% BSA was added to quench formaldehyde. After being washed by Wash Buffer, the cell pellet obtained after centrifugation was used for ultrathin nuclear cryosection. Cryosections of 150 nm were cut with glass knives using a Leica FC7 ultra-cut cryotome, collected in sucrose droplets (2.3 M in PBS), and transferred to steel frame PEN (polyethylene naphthalate) membrane slides (Leica) for ultraviolet treatment for 45 minutes prior to use. Slides were washed in 1× PBS (three times, 5 minutes each), then with nuclease-free water (three times, 5 minutes each). Cresyl violet staining was performed with sterile-filtered cresyl violet (1 % w/v in water, Sigma-Aldrich, C5042) for 15 minutes, followed by two washes with water (30 s each) and air dried for at least 15 minutes. Prepare the lysis buffer (1× single-cell lysis buffer (refer to the recipe mentioned in Hi-C library generation), 6 mg/mL QIAGEN Protease ) in advance and add it to the 0.2 mL PCR tube caps (10 μL each) (Axygen). On average, three nuclear profiles were precisely microdissected using the Leica LMD7000 laser microdissection system and deposited into each 0.2 mL PCR tube cap. The tubes can be stored inverted at 4 °C for some days before whole genome amplification.
Whole genome amplification of GAM sample DNA from microdissected nuclear profiles was performed with in-house protocol. In brief, NPs were lysed directly in the PCR caps for 4 hours at 60 °C. After protease inactivation at 75 °C for 30 min, the PCR tubes were inverted and centrifuged the right way up at 3,000 ×g for 5 minutes to collect the extracted DNA in the bottom of the tube. 10 μL of each lysed sample was transposed by the addition of 20 μL Transposition Mix (15 μL 2× Transposition buffer (20 mM 1 M Tris pH 8.0, 10 mM 1 M MgCl2, 16% w/v PEG8000), 0.2 μL TTE Mix V50 (dilute by a factor of 100) (Vazyme TD501), 4.8 μL water) and incubated at 55 °C for 10 minutes. Transposition was stopped by adding 1.5 μL SDS mix (0.9 μL 200mM EDTA, 0.6 uL 2% SDS) and then the tube was incubated at 55 °C for 10 minutes. Then, 1 μL 25 μM i5 unique dual (UD) index and 1 μL 25 μM i7 unique dual (UD) index were added in each tube. Next, the samples were amplified for 26 cycles of PCR with 16.5 μL KAPA PCR mix (Roche, KK2102) (10 μL 5× KAPA HiFi GC Buffer, 1.5 μL dNTP Mix (10 mM each), 2.5 μL 25mM MgCl2, 1 μL KAPA HiFi DNA Polymerase (1 U/μL) and 1.5 μL water). The barcoded nuclear profile libraries were then pooled and purified with 0.9× VAHTS DNA Clean Beads. The final libraries were sequenced with paired-end 150bp reads on a NovaSeq X plus (Illumina) platform according to the manufacturer’s instructions.

value: Sperm cells


title: HUG9_HGNP590, Human sperm, 3NP GAM


title: HCT116_p53_sicontrol_24h_batch1_rep3

description: Microarray data from HCT116 p53+/+ transfected with siCtrl 24h

value: siCtrl


title: 3984_ST

description:
Library name: 3984_breast_cancer_ST
Fresh frozen Spatial transcriptome sequencing


title: HCT116_p53_sicontrol_24h_batch1_rep2


title: exosome at Con, biological rep1


title: exosome at T1, biological rep2

source_name: A673 derived exosome at 48h

value: Ewing sarcoma cell line A673


title: exosome at T1, biological rep3


title: exosome at T1, biological rep1


title: HCT116_p53_sicontrol_24h_batch1_rep1


title: exosome at T2, biological rep1


title: HUG9_HGNP589, Human sperm, 3NP GAM


title: HUG9_HGNP587, Human sperm, 3NP GAM


title: HUG9_HGNP588, Human sperm, 3NP GAM


title: HUG9_HGNP586, Human sperm, 3NP GAM


title: HUG9_HGNP585, Human sperm, 3NP GAM


title: HUG9_HGNP584, Human sperm, 3NP GAM


title: HUG9_HGNP583, Human sperm, 3NP GAM


title: HUG9_HGNP581, Human sperm, 3NP GAM


title: HUG9_HGNP582, Human sperm, 3NP GAM


title: HUG9_HGNP580, Human sperm, 3NP GAM


title: HUG9_HGNP579, Human sperm, 3NP GAM


title: HUG9_HGNP578, Human sperm, 3NP GAM


title: HUG9_HGNP577, Human sperm, 3NP GAM


title: HCT116_p53_sicontrol_48h_batch1_rep3


title: HUG9_HGNP575, Human sperm, 3NP GAM


title: HUG9_HGNP576, Human sperm, 3NP GAM


title: HUG9_HGNP574, Human sperm, 3NP GAM


title: HUG9_HGNP573, Human sperm, 3NP GAM


title: HUG9_HGNP572, Human sperm, 3NP GAM


title: HUG9_HGNP570, Human sperm, 3NP GAM


title: HUG9_HGNP571, Human sperm, 3NP GAM


title: HUG9_HGNP569, Human sperm, 3NP GAM


title: HUG9_HGNP566, Human sperm, 3NP GAM


title: HUG9_HGNP560, Human sperm, 3NP GAM


title: HUG9_HGNP564, Human sperm, 3NP GAM


title: HUG9_HGNP565, Human sperm, 3NP GAM


title: HUG9_HGNP558, Human sperm, 3NP GAM


title: HUG9_HGNP561, Human sperm, 3NP GAM


title: HUG9_HGNP562, Human sperm, 3NP GAM


title: HUG9_HGNP567, Human sperm, 3NP GAM


title: HUG9_HGNP563, Human sperm, 3NP GAM


title: HUG9_HGNP557, Human sperm, 3NP GAM


title: HUG9_HGNP559, Human sperm, 3NP GAM


title: HUG8_HGNP555, Human sperm, 3NP GAM


title: HUG8_HGNP556, Human sperm, 3NP GAM


title: HUG8_HGNP552, Human sperm, 3NP GAM


title: HUG8_HGNP553, Human sperm, 3NP GAM


title: HUG8_HGNP554, Human sperm, 3NP GAM


title: HUG8_HGNP551, Human sperm, 3NP GAM


title: HUG8_HGNP550, Human sperm, 3NP GAM


title: HUG8_HGNP547, Human sperm, 3NP GAM


title: HUG8_HGNP545, Human sperm, 3NP GAM


title: HUG8_HGNP549, Human sperm, 3NP GAM


title: HUG8_HGNP546, Human sperm, 3NP GAM


title: HUG9_HGNP568, Human sperm, 3NP GAM


title: HUG8_HGNP542, Human sperm, 3NP GAM


title: HUG8_HGNP540, Human sperm, 3NP GAM


title: HUG8_HGNP541, Human sperm, 3NP GAM


title: HUG8_HGNP543, Human sperm, 3NP GAM


title: HUG8_HGNP548, Human sperm, 3NP GAM


title: HUG8_HGNP538, Human sperm, 3NP GAM


title: HUG8_HGNP537, Human sperm, 3NP GAM


title: HUG8_HGNP536, Human sperm, 3NP GAM


title: HUG8_HGNP534, Human sperm, 3NP GAM


title: HUG8_HGNP530, Human sperm, 3NP GAM


title: HUG8_HGNP529, Human sperm, 3NP GAM


title: HUG8_HGNP527, Human sperm, 3NP GAM


title: HUG8_HGNP531, Human sperm, 3NP GAM


title: HUG8_HGNP528, Human sperm, 3NP GAM


title: HUG8_HGNP533, Human sperm, 3NP GAM


title: HUG8_HGNP524, Human sperm, 3NP GAM


title: HUG8_HGNP523, Human sperm, 3NP GAM


title: HUG8_HGNP522, Human sperm, 3NP GAM


title: HUG8_HGNP525, Human sperm, 3NP GAM


title: HUG8_HGNP544, Human sperm, 3NP GAM


title: HUG8_HGNP539, Human sperm, 3NP GAM


title: HUG8_HGNP521, Human sperm, 3NP GAM


title: HUG8_HGNP519, Human sperm, 3NP GAM


title: HUG8_HGNP514, Human sperm, 3NP GAM


title: HUG8_HGNP518, Human sperm, 3NP GAM


title: HUG8_HGNP535, Human sperm, 3NP GAM


title: HUG8_HGNP516, Human sperm, 3NP GAM


title: HUG8_HGNP520, Human sperm, 3NP GAM


title: HUG8_HGNP517, Human sperm, 3NP GAM


title: HUG8_HGNP509, Human sperm, 3NP GAM


title: HUG8_HGNP510, Human sperm, 3NP GAM


title: HUG8_HGNP512, Human sperm, 3NP GAM


title: HUG12_HGNP832, Human sperm, 3NP GAM


title: HUG12_HGNP830, Human sperm, 3NP GAM


title: HUG12_HGNP828, Human sperm, 3NP GAM


title: HUG12_HGNP825, Human sperm, 3NP GAM


title: HUG12_HGNP827, Human sperm, 3NP GAM


title: HUG8_HGNP511, Human sperm, 3NP GAM


title: HUG8_HGNP513, Human sperm, 3NP GAM


title: HUG8_HGNP515, Human sperm, 3NP GAM


title: HUG12_HGNP829, Human sperm, 3NP GAM


title: HUG12_HGNP831, Human sperm, 3NP GAM


title: HUG12_HGNP826, Human sperm, 3NP GAM


title: HUG12_HGNP824, Human sperm, 3NP GAM


title: HUG8_HGNP532, Human sperm, 3NP GAM


title: HUG8_HGNP526, Human sperm, 3NP GAM


title: HUG12_HGNP822, Human sperm, 3NP GAM


title: HUG12_HGNP820, Human sperm, 3NP GAM


title: HUG12_HGNP821, Human sperm, 3NP GAM


title: HUG12_HGNP818, Human sperm, 3NP GAM


title: HUG12_HGNP816, Human sperm, 3NP GAM


title: HUG12_HGNP814, Human sperm, 3NP GAM


title: HUG12_HGNP819, Human sperm, 3NP GAM


title: HUG12_HGNP813, Human sperm, 3NP GAM


title: HUG12_HGNP809, Human sperm, 3NP GAM


title: HUG12_HGNP817, Human sperm, 3NP GAM


title: HUG12_HGNP823, Human sperm, 3NP GAM


title: HUG12_HGNP808, Human sperm, 3NP GAM


title: HUG12_HGNP804, Human sperm, 3NP GAM


title: HUG12_HGNP803, Human sperm, 3NP GAM


title: HUG12_HGNP806, Human sperm, 3NP GAM


title: HUG12_HGNP811, Human sperm, 3NP GAM


title: HUG12_HGNP794, Human sperm, 3NP GAM


title: HUG12_HGNP799, Human sperm, 3NP GAM


title: HUG12_HGNP798, Human sperm, 3NP GAM


title: HUG12_HGNP802, Human sperm, 3NP GAM


title: HUG12_HGNP793, Human sperm, 3NP GAM


title: HUG12_HGNP792, Human sperm, 3NP GAM


title: HUG12_HGNP797, Human sperm, 3NP GAM


title: HUG12_HGNP800, Human sperm, 3NP GAM


title: HUG12_HGNP810, Human sperm, 3NP GAM


title: HUG12_HGNP812, Human sperm, 3NP GAM


title: HUG12_HGNP795, Human sperm, 3NP GAM


title: HUG12_HGNP807, Human sperm, 3NP GAM


title: HUG12_HGNP791, Human sperm, 3NP GAM


title: HUG12_HGNP796, Human sperm, 3NP GAM


title: HUG12_HGNP815, Human sperm, 3NP GAM


title: HUG12_HGNP790, Human sperm, 3NP GAM


title: HUG12_HGNP789, Human sperm, 3NP GAM


title: HUG12_HGNP805, Human sperm, 3NP GAM


title: HUG12_HGNP801, Human sperm, 3NP GAM


title: HUG12_HGNP788, Human sperm, 3NP GAM


title: HUG12_HGNP786, Human sperm, 3NP GAM


title: HUG12_HGNP787, Human sperm, 3NP GAM


title: HUG12_HGNP785, Human sperm, 3NP GAM


title: HUG12_HGNP783, Human sperm, 3NP GAM


title: HUG12_HGNP781, Human sperm, 3NP GAM


title: HUG12_HGNP784, Human sperm, 3NP GAM


title: HUG12_HGNP782, Human sperm, 3NP GAM


title: HUG12_HGNP780, Human sperm, 3NP GAM


title: HUG12_HGNP779, Human sperm, 3NP GAM


title: HUG12_HGNP772, Human sperm, 3NP GAM


title: HUG12_HGNP776, Human sperm, 3NP GAM


title: HUG12_HGNP774, Human sperm, 3NP GAM


title: HUG12_HGNP771, Human sperm, 3NP GAM


title: HUG12_HGNP777, Human sperm, 3NP GAM


title: HUG12_HGNP775, Human sperm, 3NP GAM


title: HUG12_HGNP773, Human sperm, 3NP GAM


title: HUG12_HGNP769, Human sperm, 3NP GAM


title: HUG12_HGNP766, Human sperm, 3NP GAM


title: HUG12_HGNP767, Human sperm, 3NP GAM


title: HUG12_HGNP768, Human sperm, 3NP GAM


title: HUG12_HGNP764, Human sperm, 3NP GAM


title: HUG12_HGNP763, Human sperm, 3NP GAM


title: HUG12_HGNP762, Human sperm, 3NP GAM


title: HUG12_HGNP758, Human sperm, 3NP GAM


title: HUG12_HGNP760, Human sperm, 3NP GAM


title: HUG12_HGNP761, Human sperm, 3NP GAM


title: HUG12_HGNP759, Human sperm, 3NP GAM


title: HUG12_HGNP778, Human sperm, 3NP GAM


title: HUG12_HGNP756, Human sperm, 3NP GAM


title: HUG12_HGNP755, Human sperm, 3NP GAM


title: HUG12_HGNP757, Human sperm, 3NP GAM


title: HUG12_HGNP765, Human sperm, 3NP GAM


title: HUG12_HGNP751, Human sperm, 3NP GAM


title: HUG12_HGNP748, Human sperm, 3NP GAM


title: HUG12_HGNP753, Human sperm, 3NP GAM


title: HUG12_HGNP754, Human sperm, 3NP GAM


title: HUG12_HGNP752, Human sperm, 3NP GAM


title: HUG11_HGNP747, Human sperm, 3NP GAM


title: HUG12_HGNP750, Human sperm, 3NP GAM


title: HUG12_HGNP770, Human sperm, 3NP GAM


title: HUG11_HGNP746, Human sperm, 3NP GAM


title: HUG11_HGNP739, Human sperm, 3NP GAM


title: HUG11_HGNP745, Human sperm, 3NP GAM


title: HUG11_HGNP742, Human sperm, 3NP GAM


title: HUG11_HGNP743, Human sperm, 3NP GAM


title: HUG11_HGNP740, Human sperm, 3NP GAM


title: HUG11_HGNP744, Human sperm, 3NP GAM


title: HUG11_HGNP737, Human sperm, 3NP GAM


title: HUG11_HGNP736, Human sperm, 3NP GAM


title: HUG11_HGNP732, Human sperm, 3NP GAM


title: HUG11_HGNP731, Human sperm, 3NP GAM


title: HUG11_HGNP734, Human sperm, 3NP GAM


title: HUG11_HGNP735, Human sperm, 3NP GAM


title: HUG11_HGNP733, Human sperm, 3NP GAM


title: HUG12_HGNP749, Human sperm, 3NP GAM


title: HUG11_HGNP730, Human sperm, 3NP GAM


title: HUG11_HGNP727, Human sperm, 3NP GAM


title: HUG11_HGNP728, Human sperm, 3NP GAM


title: HUG11_HGNP729, Human sperm, 3NP GAM


title: HUG11_HGNP726, Human sperm, 3NP GAM


title: HUG11_HGNP741, Human sperm, 3NP GAM


title: HUG11_HGNP724, Human sperm, 3NP GAM


title: HUG11_HGNP721, Human sperm, 3NP GAM


title: HUG11_HGNP720, Human sperm, 3NP GAM


title: HUG11_HGNP725, Human sperm, 3NP GAM


title: HUG11_HGNP723, Human sperm, 3NP GAM


title: HUG11_HGNP716, Human sperm, 3NP GAM


title: HUG11_HGNP712, Human sperm, 3NP GAM


title: HUG11_HGNP738, Human sperm, 3NP GAM


title: HUG11_HGNP722, Human sperm, 3NP GAM


title: HUG11_HGNP717, Human sperm, 3NP GAM


title: HUG11_HGNP709, Human sperm, 3NP GAM


title: HUG11_HGNP710, Human sperm, 3NP GAM


title: HUG11_HGNP715, Human sperm, 3NP GAM


title: HUG11_HGNP711, Human sperm, 3NP GAM


title: HUG11_HGNP718, Human sperm, 3NP GAM


title: HUG11_HGNP708, Human sperm, 3NP GAM


title: HUG11_HGNP703, Human sperm, 3NP GAM


title: HUG11_HGNP707, Human sperm, 3NP GAM


title: HUG11_HGNP706, Human sperm, 3NP GAM


title: HUG11_HGNP704, Human sperm, 3NP GAM


title: HUG11_HGNP702, Human sperm, 3NP GAM


title: HUG11_HGNP700, Human sperm, 3NP GAM


title: HUG11_HGNP701, Human sperm, 3NP GAM


title: HUG11_HGNP719, Human sperm, 3NP GAM


title: HUG11_HGNP714, Human sperm, 3NP GAM


title: HUG11_HGNP697, Human sperm, 3NP GAM


title: HUG11_HGNP699, Human sperm, 3NP GAM


title: HUG11_HGNP698, Human sperm, 3NP GAM


title: HUG11_HGNP696, Human sperm, 3NP GAM


title: HUG11_HGNP694, Human sperm, 3NP GAM


title: HUG11_HGNP713, Human sperm, 3NP GAM


title: HUG11_HGNP693, Human sperm, 3NP GAM


title: HUG11_HGNP687, Human sperm, 3NP GAM


title: HUG11_HGNP686, Human sperm, 3NP GAM


title: HUG11_HGNP689, Human sperm, 3NP GAM


title: HUG11_HGNP685, Human sperm, 3NP GAM


title: HUG11_HGNP691, Human sperm, 3NP GAM


title: HUG11_HGNP683, Human sperm, 3NP GAM


title: HUG11_HGNP688, Human sperm, 3NP GAM


title: HUG11_HGNP682, Human sperm, 3NP GAM


title: HUG11_HGNP705, Human sperm, 3NP GAM


title: HUG11_HGNP684, Human sperm, 3NP GAM


title: HUG11_HGNP680, Human sperm, 3NP GAM


title: HUG11_HGNP678, Human sperm, 3NP GAM


title: HUG11_HGNP679, Human sperm, 3NP GAM


title: HUG11_HGNP677, Human sperm, 3NP GAM


title: HUG11_HGNP676, Human sperm, 3NP GAM


title: HUG11_HGNP674, Human sperm, 3NP GAM


title: HUG11_HGNP675, Human sperm, 3NP GAM


title: HUG11_HGNP695, Human sperm, 3NP GAM


title: HUG11_HGNP673, Human sperm, 3NP GAM


title: HUG11_HGNP670, Human sperm, 3NP GAM


title: HUG11_HGNP692, Human sperm, 3NP GAM


title: HUG11_HGNP690, Human sperm, 3NP GAM


title: HUG11_HGNP669, Human sperm, 3NP GAM


title: HUG11_HGNP672, Human sperm, 3NP GAM


title: HUG11_HGNP671, Human sperm, 3NP GAM


title: HUG11_HGNP666, Human sperm, 3NP GAM


title: HUG11_HGNP668, Human sperm, 3NP GAM


title: HUG11_HGNP665, Human sperm, 3NP GAM


title: HUG11_HGNP663, Human sperm, 3NP GAM


title: HUG11_HGNP662, Human sperm, 3NP GAM


title: HUG11_HGNP664, Human sperm, 3NP GAM


title: HUG11_HGNP660, Human sperm, 3NP GAM


title: HUG11_HGNP658, Human sperm, 3NP GAM


title: HUG11_HGNP661, Human sperm, 3NP GAM


title: HUG11_HGNP657, Human sperm, 3NP GAM


title: HUG11_HGNP681, Human sperm, 3NP GAM


title: HUG11_HGNP659, Human sperm, 3NP GAM


title: HUG11_HGNP655, Human sperm, 3NP GAM


title: HUG11_HGNP656, Human sperm, 3NP GAM


title: HUG11_HGNP653, Human sperm, 3NP GAM


title: HUG10_HGNP650, Human sperm, 3NP GAM


title: HUG11_HGNP652, Human sperm, 3NP GAM


title: HUG11_HGNP654, Human sperm, 3NP GAM


title: HUG10_HGNP651, Human sperm, 3NP GAM


title: HUG10_HGNP649, Human sperm, 3NP GAM


title: HUG10_HGNP648, Human sperm, 3NP GAM


title: HUG10_HGNP646, Human sperm, 3NP GAM


title: HUG11_HGNP667, Human sperm, 3NP GAM


title: HUG10_HGNP641, Human sperm, 3NP GAM


title: HUG10_HGNP644, Human sperm, 3NP GAM


title: HUG10_HGNP645, Human sperm, 3NP GAM


title: HUG10_HGNP647, Human sperm, 3NP GAM


title: HUG10_HGNP640, Human sperm, 3NP GAM


title: HUG10_HGNP642, Human sperm, 3NP GAM


title: HUG10_HGNP643, Human sperm, 3NP GAM


title: HUG10_HGNP639, Human sperm, 3NP GAM


title: HUG10_HGNP635, Human sperm, 3NP GAM


title: HUG10_HGNP636, Human sperm, 3NP GAM


title: HUG10_HGNP634, Human sperm, 3NP GAM


title: HUG10_HGNP638, Human sperm, 3NP GAM


title: HUG10_HGNP633, Human sperm, 3NP GAM


title: HUG10_HGNP630, Human sperm, 3NP GAM


title: HUG10_HGNP632, Human sperm, 3NP GAM


title: HUG10_HGNP628, Human sperm, 3NP GAM


title: HUG10_HGNP629, Human sperm, 3NP GAM


title: HUG10_HGNP626, Human sperm, 3NP GAM


title: HUG10_HGNP624, Human sperm, 3NP GAM


title: HUG10_HGNP622, Human sperm, 3NP GAM


title: HUG10_HGNP623, Human sperm, 3NP GAM


title: HUG10_HGNP625, Human sperm, 3NP GAM


title: HUG10_HGNP621, Human sperm, 3NP GAM


title: HUG10_HGNP620, Human sperm, 3NP GAM


title: HUG10_HGNP615, Human sperm, 3NP GAM


title: HUG10_HGNP617, Human sperm, 3NP GAM


title: HUG10_HGNP614, Human sperm, 3NP GAM


title: HUG10_HGNP616, Human sperm, 3NP GAM


title: HUG10_HGNP618, Human sperm, 3NP GAM


title: HUG10_HGNP613, Human sperm, 3NP GAM


title: HUG10_HGNP637, Human sperm, 3NP GAM


title: HUG10_HGNP612, Human sperm, 3NP GAM


title: HUG10_HGNP610, Human sperm, 3NP GAM


title: HUG10_HGNP609, Human sperm, 3NP GAM


title: HUG10_HGNP611, Human sperm, 3NP GAM


title: HUG10_HGNP607, Human sperm, 3NP GAM


title: HUG10_HGNP631, Human sperm, 3NP GAM


title: HUG10_HGNP627, Human sperm, 3NP GAM


title: HUG10_HGNP605, Human sperm, 3NP GAM


title: HUG10_HGNP608, Human sperm, 3NP GAM


title: HUG10_HGNP604, Human sperm, 3NP GAM


title: HUG10_HGNP603, Human sperm, 3NP GAM


title: HUG10_HGNP602, Human sperm, 3NP GAM


title: HUG10_HGNP599, Human sperm, 3NP GAM


title: HUG10_HGNP601, Human sperm, 3NP GAM


title: HUG10_HGNP600, Human sperm, 3NP GAM


title: HUG10_HGNP595, Human sperm, 3NP GAM


title: HUG10_HGNP596, Human sperm, 3NP GAM


title: HUG10_HGNP594, Human sperm, 3NP GAM


title: HUG10_HGNP597, Human sperm, 3NP GAM


title: HUG10_HGNP598, Human sperm, 3NP GAM


title: HUG10_HGNP619, Human sperm, 3NP GAM


title: HUG10_HGNP593, Human sperm, 3NP GAM


title: HUG7_HGNP507, Human sperm, 3NP GAM


title: HUG7_HGNP505, Human sperm, 3NP GAM


title: HUG7_HGNP508, Human sperm, 3NP GAM


title: HUG10_HGNP592, Human sperm, 3NP GAM


title: HUG7_HGNP503, Human sperm, 3NP GAM


title: HUG7_HGNP504, Human sperm, 3NP GAM


title: HUG7_HGNP506, Human sperm, 3NP GAM


title: HUG7_HGNP502, Human sperm, 3NP GAM


title: HUG10_HGNP606, Human sperm, 3NP GAM


title: HUG7_HGNP498, Human sperm, 3NP GAM


title: HUG7_HGNP500, Human sperm, 3NP GAM


title: HUG7_HGNP496, Human sperm, 3NP GAM


title: HUG7_HGNP497, Human sperm, 3NP GAM


title: HUG7_HGNP494, Human sperm, 3NP GAM


title: HUG7_HGNP495, Human sperm, 3NP GAM


title: HUG7_HGNP492, Human sperm, 3NP GAM


title: HUG7_HGNP501, Human sperm, 3NP GAM


title: HUG7_HGNP491, Human sperm, 3NP GAM


title: HUG7_HGNP490, Human sperm, 3NP GAM


title: HUG7_HGNP499, Human sperm, 3NP GAM


title: HUG7_HGNP493, Human sperm, 3NP GAM


title: HUG7_HGNP489, Human sperm, 3NP GAM


title: HUG7_HGNP488, Human sperm, 3NP GAM


title: HUG7_HGNP485, Human sperm, 3NP GAM


title: HUG7_HGNP486, Human sperm, 3NP GAM


title: HUG7_HGNP487, Human sperm, 3NP GAM


title: HUG7_HGNP483, Human sperm, 3NP GAM


title: HUG7_HGNP484, Human sperm, 3NP GAM


title: HUG7_HGNP482, Human sperm, 3NP GAM


title: HUG7_HGNP480, Human sperm, 3NP GAM


title: HUG7_HGNP478, Human sperm, 3NP GAM


title: HUG7_HGNP477, Human sperm, 3NP GAM


title: HUG7_HGNP475, Human sperm, 3NP GAM


title: HUG7_HGNP479, Human sperm, 3NP GAM


title: HUG7_HGNP476, Human sperm, 3NP GAM


title: HUG7_HGNP474, Human sperm, 3NP GAM


title: HUG7_HGNP470, Human sperm, 3NP GAM


title: HUG7_HGNP471, Human sperm, 3NP GAM


title: HUG7_HGNP473, Human sperm, 3NP GAM


title: HUG7_HGNP468, Human sperm, 3NP GAM


title: HUG7_HGNP472, Human sperm, 3NP GAM


title: HUG7_HGNP464, Human sperm, 3NP GAM


title: HUG7_HGNP469, Human sperm, 3NP GAM


title: HUG7_HGNP462, Human sperm, 3NP GAM


title: HUG7_HGNP467, Human sperm, 3NP GAM


title: HUG7_HGNP461, Human sperm, 3NP GAM


title: HUG7_HGNP463, Human sperm, 3NP GAM


title: HUG7_HGNP465, Human sperm, 3NP GAM


title: HUG7_HGNP458, Human sperm, 3NP GAM


title: HUG7_HGNP460, Human sperm, 3NP GAM


title: HUG7_HGNP455, Human sperm, 3NP GAM


title: HUG7_HGNP457, Human sperm, 3NP GAM


title: HUG7_HGNP481, Human sperm, 3NP GAM


title: HUG7_HGNP453, Human sperm, 3NP GAM


title: HUG7_HGNP452, Human sperm, 3NP GAM


title: HUG7_HGNP459, Human sperm, 3NP GAM


title: HUG7_HGNP451, Human sperm, 3NP GAM


title: HUG7_HGNP449, Human sperm, 3NP GAM


title: HUG7_HGNP446, Human sperm, 3NP GAM


title: HUG7_HGNP445, Human sperm, 3NP GAM


title: HUG7_HGNP448, Human sperm, 3NP GAM


title: HUG7_HGNP447, Human sperm, 3NP GAM


title: HUG7_HGNP444, Human sperm, 3NP GAM


title: HUG7_HGNP466, Human sperm, 3NP GAM


title: HUG7_HGNP443, Human sperm, 3NP GAM


title: HUG7_HGNP439, Human sperm, 3NP GAM


title: HUG7_HGNP440, Human sperm, 3NP GAM


title: HUG7_HGNP438, Human sperm, 3NP GAM


title: HUG7_HGNP442, Human sperm, 3NP GAM


title: HUG7_HGNP441, Human sperm, 3NP GAM


title: HUG7_HGNP437, Human sperm, 3NP GAM


title: HUG7_HGNP436, Human sperm, 3NP GAM


title: HUG7_HGNP434, Human sperm, 3NP GAM


title: HUG7_HGNP432, Human sperm, 3NP GAM


title: HUG7_HGNP454, Human sperm, 3NP GAM


title: HUG7_HGNP433, Human sperm, 3NP GAM


title: HUG7_HGNP431, Human sperm, 3NP GAM


title: HUG7_HGNP435, Human sperm, 3NP GAM


title: HUG7_HGNP429, Human sperm, 3NP GAM


title: HUG7_HGNP423, Human sperm, 3NP GAM


title: HUG7_HGNP427, Human sperm, 3NP GAM


title: HUG7_HGNP426, Human sperm, 3NP GAM


title: HUG7_HGNP428, Human sperm, 3NP GAM


title: HUG7_HGNP425, Human sperm, 3NP GAM


title: HUG7_HGNP450, Human sperm, 3NP GAM


title: HUG7_HGNP422, Human sperm, 3NP GAM


title: HUG7_HGNP420, Human sperm, 3NP GAM


title: HUG7_HGNP419, Human sperm, 3NP GAM


title: HUG7_HGNP421, Human sperm, 3NP GAM


title: HUG7_HGNP418, Human sperm, 3NP GAM


title: HUG7_HGNP417, Human sperm, 3NP GAM


title: HUG7_HGNP416, Human sperm, 3NP GAM


title: HUG7_HGNP415, Human sperm, 3NP GAM


title: HUG7_HGNP413, Human sperm, 3NP GAM


title: HUG6_HGNP410, Human sperm, 3NP GAM


title: HUG6_HGNP412, Human sperm, 3NP GAM


title: HUG7_HGNP414, Human sperm, 3NP GAM


title: HUG6_HGNP409, Human sperm, 3NP GAM


title: HUG6_HGNP408, Human sperm, 3NP GAM


title: HUG7_HGNP430, Human sperm, 3NP GAM


title: HUG6_HGNP405, Human sperm, 3NP GAM


title: HUG6_HGNP407, Human sperm, 3NP GAM


title: HUG7_HGNP424, Human sperm, 3NP GAM


title: HUG6_HGNP403, Human sperm, 3NP GAM


title: HUG6_HGNP402, Human sperm, 3NP GAM


title: HUG6_HGNP406, Human sperm, 3NP GAM


title: HUG6_HGNP399, Human sperm, 3NP GAM


title: HUG6_HGNP398, Human sperm, 3NP GAM


title: HUG6_HGNP400, Human sperm, 3NP GAM


title: HUG6_HGNP396, Human sperm, 3NP GAM


title: HUG6_HGNP395, Human sperm, 3NP GAM


title: HUG6_HGNP391, Human sperm, 3NP GAM


title: HUG6_HGNP394, Human sperm, 3NP GAM


title: HUG6_HGNP392, Human sperm, 3NP GAM


title: HUG6_HGNP389, Human sperm, 3NP GAM


title: HUG6_HGNP393, Human sperm, 3NP GAM


title: HUG6_HGNP386, Human sperm, 3NP GAM


title: HUG6_HGNP390, Human sperm, 3NP GAM


title: HUG6_HGNP387, Human sperm, 3NP GAM


title: HUG6_HGNP385, Human sperm, 3NP GAM


title: HUG6_HGNP383, Human sperm, 3NP GAM


title: HUG6_HGNP388, Human sperm, 3NP GAM


title: HUG6_HGNP384, Human sperm, 3NP GAM


title: HUG6_HGNP411, Human sperm, 3NP GAM


title: HUG6_HGNP404, Human sperm, 3NP GAM


title: HUG6_HGNP380, Human sperm, 3NP GAM


title: HUG6_HGNP379, Human sperm, 3NP GAM


title: HUG6_HGNP378, Human sperm, 3NP GAM


title: HUG6_HGNP381, Human sperm, 3NP GAM


title: HUG6_HGNP376, Human sperm, 3NP GAM


title: HUG6_HGNP375, Human sperm, 3NP GAM


title: HUG6_HGNP374, Human sperm, 3NP GAM


title: HUG6_HGNP397, Human sperm, 3NP GAM


title: HUG6_HGNP382, Human sperm, 3NP GAM


title: HUG6_HGNP377, Human sperm, 3NP GAM


title: HUG5_HGNP371, Human sperm, 3NP GAM


title: HUG6_HGNP401, Human sperm, 3NP GAM


title: HUG5_HGNP373, Human sperm, 3NP GAM


title: HUG5_HGNP369, Human sperm, 3NP GAM


title: HUG5_HGNP370, Human sperm, 3NP GAM


title: HUG5_HGNP368, Human sperm, 3NP GAM


title: HUG5_HGNP366, Human sperm, 3NP GAM


title: HUG5_HGNP367, Human sperm, 3NP GAM


title: HUG5_HGNP365, Human sperm, 3NP GAM


title: HUG5_HGNP364, Human sperm, 3NP GAM


title: HUG5_HGNP363, Human sperm, 3NP GAM


title: HUG5_HGNP361, Human sperm, 3NP GAM


title: HUG5_HGNP362, Human sperm, 3NP GAM


title: HUG5_HGNP357, Human sperm, 3NP GAM


title: HUG5_HGNP359, Human sperm, 3NP GAM


title: HUG5_HGNP360, Human sperm, 3NP GAM


title: HUG5_HGNP358, Human sperm, 3NP GAM


title: HUG5_HGNP356, Human sperm, 3NP GAM


title: HUG5_HGNP354, Human sperm, 3NP GAM


title: HUG5_HGNP355, Human sperm, 3NP GAM


title: HUG5_HGNP353, Human sperm, 3NP GAM


title: HUG5_HGNP349, Human sperm, 3NP GAM


title: HUG5_HGNP372, Human sperm, 3NP GAM


title: HUG5_HGNP347, Human sperm, 3NP GAM


title: HUG5_HGNP345, Human sperm, 3NP GAM


title: HUG5_HGNP351, Human sperm, 3NP GAM


title: HUG5_HGNP350, Human sperm, 3NP GAM


title: HUG5_HGNP343, Human sperm, 3NP GAM


title: HUG5_HGNP346, Human sperm, 3NP GAM


title: HUG5_HGNP342, Human sperm, 3NP GAM


title: HUG5_HGNP341, Human sperm, 3NP GAM


title: HUG5_HGNP344, Human sperm, 3NP GAM


title: HUG5_HGNP339, Human sperm, 3NP GAM


title: HUG5_HGNP338, Human sperm, 3NP GAM


title: HUG5_HGNP336, Human sperm, 3NP GAM


title: HUG5_HGNP337, Human sperm, 3NP GAM


title: HUG5_HGNP335, Human sperm, 3NP GAM


title: HUG5_HGNP334, Human sperm, 3NP GAM


title: HUG5_HGNP332, Human sperm, 3NP GAM


title: HUG5_HGNP352, Human sperm, 3NP GAM


title: HUG5_HGNP331, Human sperm, 3NP GAM


title: HUG5_HGNP333, Human sperm, 3NP GAM


title: HUG5_HGNP330, Human sperm, 3NP GAM


title: HUG5_HGNP348, Human sperm, 3NP GAM


title: HUG5_HGNP328, Human sperm, 3NP GAM


title: HUG5_HGNP326, Human sperm, 3NP GAM


title: HUG4_HGNP322, Human sperm, 3NP GAM


title: HUG4_HGNP325, Human sperm, 3NP GAM


title: HUG4_HGNP320, Human sperm, 3NP GAM


title: HUG5_HGNP327, Human sperm, 3NP GAM


title: HUG5_HGNP340, Human sperm, 3NP GAM


title: HUG4_HGNP314, Human sperm, 3NP GAM


title: HUG4_HGNP313, Human sperm, 3NP GAM


title: HUG4_HGNP319, Human sperm, 3NP GAM


title: HUG4_HGNP316, Human sperm, 3NP GAM


title: HUG4_HGNP321, Human sperm, 3NP GAM


title: HUG4_HGNP318, Human sperm, 3NP GAM


title: HUG4_HGNP324, Human sperm, 3NP GAM


title: HUG4_HGNP311, Human sperm, 3NP GAM


title: HUG4_HGNP309, Human sperm, 3NP GAM


title: HUG4_HGNP317, Human sperm, 3NP GAM


title: HUG4_HGNP308, Human sperm, 3NP GAM


title: HUG5_HGNP329, Human sperm, 3NP GAM


title: HUG4_HGNP307, Human sperm, 3NP GAM


title: HUG4_HGNP306, Human sperm, 3NP GAM


title: HUG4_HGNP323, Human sperm, 3NP GAM


title: HUG4_HGNP305, Human sperm, 3NP GAM


title: HUG4_HGNP304, Human sperm, 3NP GAM


title: HUG4_HGNP302, Human sperm, 3NP GAM


title: HUG4_HGNP299, Human sperm, 3NP GAM


title: HUG4_HGNP301, Human sperm, 3NP GAM


title: HUG4_HGNP296, Human sperm, 3NP GAM


title: HUG4_HGNP297, Human sperm, 3NP GAM


title: HUG4_HGNP300, Human sperm, 3NP GAM


title: HUG4_HGNP295, Human sperm, 3NP GAM


title: HUG4_HGNP298, Human sperm, 3NP GAM


title: HUG4_HGNP293, Human sperm, 3NP GAM


title: HUG4_HGNP294, Human sperm, 3NP GAM


title: HUG4_HGNP290, Human sperm, 3NP GAM


title: HUG4_HGNP312, Human sperm, 3NP GAM


title: HUG4_HGNP289, Human sperm, 3NP GAM


title: HUG4_HGNP292, Human sperm, 3NP GAM


title: HUG4_HGNP287, Human sperm, 3NP GAM


title: HUG4_HGNP310, Human sperm, 3NP GAM


title: HUG4_HGNP288, Human sperm, 3NP GAM


title: HUG4_HGNP285, Human sperm, 3NP GAM


title: HUG4_HGNP284, Human sperm, 3NP GAM


title: HUG4_HGNP283, Human sperm, 3NP GAM


title: HUG4_HGNP303, Human sperm, 3NP GAM


title: HUG4_HGNP282, Human sperm, 3NP GAM


title: HUG4_HGNP281, Human sperm, 3NP GAM


title: HUG3_HGNP276, Human sperm, 3NP GAM


title: HUG4_HGNP278, Human sperm, 3NP GAM


title: HUG4_HGNP279, Human sperm, 3NP GAM


title: HUG3_HGNP275, Human sperm, 3NP GAM


title: HUG3_HGNP273, Human sperm, 3NP GAM


title: HUG3_HGNP272, Human sperm, 3NP GAM


title: HUG3_HGNP271, Human sperm, 3NP GAM


title: HUG3_HGNP269, Human sperm, 3NP GAM


title: HUG3_HGNP274, Human sperm, 3NP GAM


title: HUG4_HGNP291, Human sperm, 3NP GAM


title: HUG3_HGNP270, Human sperm, 3NP GAM


title: HUG3_HGNP268, Human sperm, 3NP GAM


title: HUG3_HGNP266, Human sperm, 3NP GAM


title: HUG3_HGNP265, Human sperm, 3NP GAM


title: HUG4_HGNP286, Human sperm, 3NP GAM


title: HUG3_HGNP264, Human sperm, 3NP GAM


title: HUG3_HGNP263, Human sperm, 3NP GAM


title: HUG4_HGNP315, Human sperm, 3NP GAM


title: HUG3_HGNP262, Human sperm, 3NP GAM


title: HUG3_HGNP261, Human sperm, 3NP GAM


title: HUG3_HGNP267, Human sperm, 3NP GAM


title: HUG3_HGNP257, Human sperm, 3NP GAM


title: HUG3_HGNP256, Human sperm, 3NP GAM


title: HUG4_HGNP280, Human sperm, 3NP GAM


title: HUG3_HGNP255, Human sperm, 3NP GAM


title: HUG3_HGNP277, Human sperm, 3NP GAM


title: HUG3_HGNP253, Human sperm, 3NP GAM


title: HUG3_HGNP251, Human sperm, 3NP GAM


title: HUG3_HGNP254, Human sperm, 3NP GAM


title: HUG3_HGNP252, Human sperm, 3NP GAM


title: HUG3_HGNP250, Human sperm, 3NP GAM


title: HUG3_HGNP248, Human sperm, 3NP GAM


title: HUG3_HGNP249, Human sperm, 3NP GAM


title: HUG3_HGNP247, Human sperm, 3NP GAM


title: HUG3_HGNP246, Human sperm, 3NP GAM


title: HUG3_HGNP258, Human sperm, 3NP GAM


title: HUG3_HGNP244, Human sperm, 3NP GAM


title: HUG3_HGNP245, Human sperm, 3NP GAM


title: HUG3_HGNP243, Human sperm, 3NP GAM


title: HUG3_HGNP239, Human sperm, 3NP GAM


title: HUG3_HGNP259, Human sperm, 3NP GAM


title: HUG3_HGNP260, Human sperm, 3NP GAM


title: HUG3_HGNP240, Human sperm, 3NP GAM


title: HUG3_HGNP241, Human sperm, 3NP GAM


title: HUG3_HGNP235, Human sperm, 3NP GAM


title: HUG3_HGNP236, Human sperm, 3NP GAM


title: HUG3_HGNP242, Human sperm, 3NP GAM


title: HUG3_HGNP234, Human sperm, 3NP GAM


title: HUG3_HGNP238, Human sperm, 3NP GAM


title: HUG3_HGNP237, Human sperm, 3NP GAM


title: HUG3_HGNP233, Human sperm, 3NP GAM


title: HUG3_HGNP231, Human sperm, 3NP GAM


title: HUG3_HGNP229, Human sperm, 3NP GAM


title: HUG3_HGNP230, Human sperm, 3NP GAM


title: HUG3_HGNP228, Human sperm, 3NP GAM


title: HUG3_HGNP227, Human sperm, 3NP GAM


title: HUG3_HGNP224, Human sperm, 3NP GAM


title: HUG3_HGNP226, Human sperm, 3NP GAM


title: HUG3_HGNP222, Human sperm, 3NP GAM


title: HUG3_HGNP221, Human sperm, 3NP GAM


title: HUG3_HGNP223, Human sperm, 3NP GAM


title: HUG3_HGNP220, Human sperm, 3NP GAM


title: HUG3_HGNP217, Human sperm, 3NP GAM


title: HUG3_HGNP216, Human sperm, 3NP GAM


title: HUG3_HGNP218, Human sperm, 3NP GAM


title: HUG3_HGNP219, Human sperm, 3NP GAM


title: HUG3_HGNP213, Human sperm, 3NP GAM


title: HUG3_HGNP214, Human sperm, 3NP GAM


title: HUG3_HGNP212, Human sperm, 3NP GAM


title: HUG3_HGNP215, Human sperm, 3NP GAM


title: HUG3_HGNP209, Human sperm, 3NP GAM


title: HUG3_HGNP208, Human sperm, 3NP GAM


title: HUG3_HGNP232, Human sperm, 3NP GAM


title: HUG3_HGNP205, Human sperm, 3NP GAM


title: HUG3_HGNP206, Human sperm, 3NP GAM


title: HUG3_HGNP203, Human sperm, 3NP GAM


title: HUG3_HGNP211, Human sperm, 3NP GAM


title: HUG3_HGNP201, Human sperm, 3NP GAM


title: HUG3_HGNP202, Human sperm, 3NP GAM


title: HUG3_HGNP200, Human sperm, 3NP GAM


title: HUG3_HGNP199, Human sperm, 3NP GAM


title: HUG3_HGNP225, Human sperm, 3NP GAM


title: HUG3_HGNP197, Human sperm, 3NP GAM


title: HUG3_HGNP196, Human sperm, 3NP GAM


title: HUG3_HGNP194, Human sperm, 3NP GAM


title: HUG3_HGNP193, Human sperm, 3NP GAM


title: HUG3_HGNP195, Human sperm, 3NP GAM


title: HUG3_HGNP192, Human sperm, 3NP GAM


title: HUG3_HGNP190, Human sperm, 3NP GAM


title: HUG3_HGNP189, Human sperm, 3NP GAM


title: HUG3_HGNP188, Human sperm, 3NP GAM


title: HUG3_HGNP191, Human sperm, 3NP GAM


title: HUG3_HGNP185, Human sperm, 3NP GAM


title: HUG3_HGNP186, Human sperm, 3NP GAM


title: HUG3_HGNP210, Human sperm, 3NP GAM


title: HUG3_HGNP183, Human sperm, 3NP GAM


title: HUG3_HGNP187, Human sperm, 3NP GAM


title: HUG3_HGNP204, Human sperm, 3NP GAM


title: HUG3_HGNP182, Human sperm, 3NP GAM


title: HUG2_HGNP179, Human sperm, 3NP GAM


title: HUG2_HGNP180, Human sperm, 3NP GAM


title: HUG3_HGNP184, Human sperm, 3NP GAM


title: HUG2_HGNP181, Human sperm, 3NP GAM


title: HUG3_HGNP198, Human sperm, 3NP GAM


title: HUG2_HGNP175, Human sperm, 3NP GAM


title: HUG2_HGNP177, Human sperm, 3NP GAM


title: HUG2_HGNP174, Human sperm, 3NP GAM


title: HUG2_HGNP178, Human sperm, 3NP GAM


title: HUG2_HGNP176, Human sperm, 3NP GAM


title: HUG2_HGNP172, Human sperm, 3NP GAM


title: HUG2_HGNP170, Human sperm, 3NP GAM


title: HUG2_HGNP165, Human sperm, 3NP GAM


title: HUG2_HGNP168, Human sperm, 3NP GAM


title: HUG2_HGNP169, Human sperm, 3NP GAM


title: HUG2_HGNP166, Human sperm, 3NP GAM


title: HUG2_HGNP167, Human sperm, 3NP GAM


title: HUG2_HGNP164, Human sperm, 3NP GAM


title: HUG2_HGNP171, Human sperm, 3NP GAM


title: HUG2_HGNP161, Human sperm, 3NP GAM


title: HUG2_HGNP162, Human sperm, 3NP GAM


title: HUG2_HGNP173, Human sperm, 3NP GAM


title: HUG2_HGNP159, Human sperm, 3NP GAM


title: HUG2_HGNP160, Human sperm, 3NP GAM


title: HUG2_HGNP157, Human sperm, 3NP GAM


title: HUG2_HGNP156, Human sperm, 3NP GAM


title: HUG2_HGNP158, Human sperm, 3NP GAM


title: HUG2_HGNP154, Human sperm, 3NP GAM


title: HUG2_HGNP152, Human sperm, 3NP GAM


title: HUG2_HGNP153, Human sperm, 3NP GAM


title: HUG2_HGNP146, Human sperm, 3NP GAM


title: HUG2_HGNP150, Human sperm, 3NP GAM


title: HUG2_HGNP149, Human sperm, 3NP GAM


title: HUG2_HGNP145, Human sperm, 3NP GAM


title: HUG2_HGNP151, Human sperm, 3NP GAM


title: HUG2_HGNP148, Human sperm, 3NP GAM


title: HUG3_HGNP207, Human sperm, 3NP GAM


title: HUG2_HGNP144, Human sperm, 3NP GAM


title: HUG2_HGNP143, Human sperm, 3NP GAM


title: HUG2_HGNP147, Human sperm, 3NP GAM


title: HUG2_HGNP163, Human sperm, 3NP GAM


title: HUG2_HGNP142, Human sperm, 3NP GAM


title: HUG2_HGNP141, Human sperm, 3NP GAM


title: HUG2_HGNP140, Human sperm, 3NP GAM


title: HUG2_HGNP138, Human sperm, 3NP GAM


title: HUG2_HGNP134, Human sperm, 3NP GAM


title: HUG2_HGNP135, Human sperm, 3NP GAM


title: HUG2_HGNP137, Human sperm, 3NP GAM


title: HUG2_HGNP133, Human sperm, 3NP GAM


title: HUG2_HGNP136, Human sperm, 3NP GAM


title: HUG2_HGNP130, Human sperm, 3NP GAM


title: HUG2_HGNP129, Human sperm, 3NP GAM


title: HUG2_HGNP131, Human sperm, 3NP GAM


title: HUG2_HGNP132, Human sperm, 3NP GAM


title: HUG2_HGNP126, Human sperm, 3NP GAM


title: HUG2_HGNP127, Human sperm, 3NP GAM


title: HUG2_HGNP125, Human sperm, 3NP GAM


title: HUG2_HGNP124, Human sperm, 3NP GAM


title: HUG2_HGNP122, Human sperm, 3NP GAM


title: HUG2_HGNP121, Human sperm, 3NP GAM


title: HUG2_HGNP120, Human sperm, 3NP GAM


title: HUG2_HGNP123, Human sperm, 3NP GAM


title: HUG2_HGNP119, Human sperm, 3NP GAM


title: HUG2_HGNP115, Human sperm, 3NP GAM


title: HUG2_HGNP118, Human sperm, 3NP GAM


title: HUG2_HGNP117, Human sperm, 3NP GAM


title: HUG2_HGNP116, Human sperm, 3NP GAM


title: HUG2_HGNP139, Human sperm, 3NP GAM


title: HUG2_HGNP113, Human sperm, 3NP GAM


title: HUG2_HGNP114, Human sperm, 3NP GAM


title: HUG2_HGNP110, Human sperm, 3NP GAM


title: HUG2_HGNP109, Human sperm, 3NP GAM


title: HUG2_HGNP108, Human sperm, 3NP GAM


title: HUG2_HGNP128, Human sperm, 3NP GAM


title: HUG2_HGNP107, Human sperm, 3NP GAM


title: HUG2_HGNP106, Human sperm, 3NP GAM


title: HUG2_HGNP105, Human sperm, 3NP GAM


title: HUG2_HGNP104, Human sperm, 3NP GAM


title: HUG2_HGNP103, Human sperm, 3NP GAM


title: HUG2_HGNP100, Human sperm, 3NP GAM


title: HUG2_HGNP099, Human sperm, 3NP GAM


title: HUG2_HGNP098, Human sperm, 3NP GAM


title: HUG2_HGNP097, Human sperm, 3NP GAM


title: HUG2_HGNP096, Human sperm, 3NP GAM


title: HUG2_HGNP094, Human sperm, 3NP GAM


title: HUG2_HGNP095, Human sperm, 3NP GAM


title: HUG2_HGNP102, Human sperm, 3NP GAM


title: HUG2_HGNP101, Human sperm, 3NP GAM


title: HUG2_HGNP092, Human sperm, 3NP GAM


title: HUG2_HGNP093, Human sperm, 3NP GAM


title: HUG1_HGNP090, Human sperm, 3NP GAM


title: HUG2_HGNP091, Human sperm, 3NP GAM


title: HUG1_HGNP089, Human sperm, 3NP GAM


title: HUG1_HGNP088, Human sperm, 3NP GAM


title: HUG1_HGNP087, Human sperm, 3NP GAM


title: HUG2_HGNP112, Human sperm, 3NP GAM


title: HUG2_HGNP111, Human sperm, 3NP GAM


title: HUG1_HGNP085, Human sperm, 3NP GAM


title: HUG1_HGNP082, Human sperm, 3NP GAM


title: HUG1_HGNP080, Human sperm, 3NP GAM


title: HUG1_HGNP079, Human sperm, 3NP GAM


title: HUG1_HGNP081, Human sperm, 3NP GAM


title: HUG1_HGNP078, Human sperm, 3NP GAM


title: HUG1_HGNP083, Human sperm, 3NP GAM


title: HUG1_HGNP074, Human sperm, 3NP GAM


title: HUG1_HGNP071, Human sperm, 3NP GAM


title: HUG1_HGNP069, Human sperm, 3NP GAM


title: HUG1_HGNP070, Human sperm, 3NP GAM


title: HUG1_HGNP077, Human sperm, 3NP GAM


title: HUG1_HGNP066, Human sperm, 3NP GAM


title: HUG1_HGNP065, Human sperm, 3NP GAM


title: HUG1_HGNP067, Human sperm, 3NP GAM


title: HUG1_HGNP068, Human sperm, 3NP GAM


title: HUG1_HGNP072, Human sperm, 3NP GAM


title: HUG1_HGNP062, Human sperm, 3NP GAM


title: HUG1_HGNP063, Human sperm, 3NP GAM


title: HUG1_HGNP061, Human sperm, 3NP GAM


title: HUG1_HGNP084, Human sperm, 3NP GAM


title: HUG1_HGNP060, Human sperm, 3NP GAM


title: HUG2_HGNP155, Human sperm, 3NP GAM


title: HUG1_HGNP058, Human sperm, 3NP GAM


title: HUG1_HGNP076, Human sperm, 3NP GAM


title: HUG1_HGNP059, Human sperm, 3NP GAM


title: HUG1_HGNP086, Human sperm, 3NP GAM


title: HUG1_HGNP056, Human sperm, 3NP GAM


title: HUG1_HGNP057, Human sperm, 3NP GAM


title: HUG1_HGNP075, Human sperm, 3NP GAM


title: HUG1_HGNP053, Human sperm, 3NP GAM


title: HUG1_HGNP052, Human sperm, 3NP GAM


title: HUG1_HGNP051, Human sperm, 3NP GAM


title: HUG1_HGNP054, Human sperm, 3NP GAM


title: HUG1_HGNP073, Human sperm, 3NP GAM


title: HUG1_HGNP048, Human sperm, 3NP GAM


title: HUG1_HGNP049, Human sperm, 3NP GAM


title: HUG1_HGNP064, Human sperm, 3NP GAM


title: HUG1_HGNP044, Human sperm, 3NP GAM


title: HUG1_HGNP050, Human sperm, 3NP GAM


title: HUG1_HGNP043, Human sperm, 3NP GAM


title: HUG1_HGNP045, Human sperm, 3NP GAM


title: HUG1_HGNP047, Human sperm, 3NP GAM


title: HUG1_HGNP042, Human sperm, 3NP GAM


title: HUG1_HGNP040, Human sperm, 3NP GAM


title: HUG1_HGNP041, Human sperm, 3NP GAM


title: HUG1_HGNP046, Human sperm, 3NP GAM


title: HUG1_HGNP036, Human sperm, 3NP GAM


title: HUG1_HGNP034, Human sperm, 3NP GAM


title: HUG1_HGNP039, Human sperm, 3NP GAM


title: HUG1_HGNP055, Human sperm, 3NP GAM


title: HUG1_HGNP038, Human sperm, 3NP GAM


title: HUG1_HGNP029, Human sperm, 3NP GAM


title: HUG1_HGNP026, Human sperm, 3NP GAM


title: HUG1_HGNP033, Human sperm, 3NP GAM


title: HUG1_HGNP032, Human sperm, 3NP GAM


title: HUG1_HGNP027, Human sperm, 3NP GAM


title: HUG1_HGNP035, Human sperm, 3NP GAM


title: HUG1_HGNP030, Human sperm, 3NP GAM


title: HUG1_HGNP031, Human sperm, 3NP GAM


title: HUG1_HGNP023, Human sperm, 3NP GAM


title: HUG1_HGNP024, Human sperm, 3NP GAM


title: HUG1_HGNP022, Human sperm, 3NP GAM


title: HUG1_HGNP019, Human sperm, 3NP GAM


title: HUG1_HGNP016, Human sperm, 3NP GAM


title: HUG1_HGNP020, Human sperm, 3NP GAM


title: HUG1_HGNP018, Human sperm, 3NP GAM


title: HUG1_HGNP017, Human sperm, 3NP GAM


title: HUG1_HGNP014, Human sperm, 3NP GAM


title: HUG1_HGNP015, Human sperm, 3NP GAM


title: HUG1_HGNP013, Human sperm, 3NP GAM


title: HUG1_HGNP010, Human sperm, 3NP GAM


title: HUG1_HGNP012, Human sperm, 3NP GAM


title: HUG1_HGNP009, Human sperm, 3NP GAM


title: HUG1_HGNP028, Human sperm, 3NP GAM


title: HUG1_HGNP025, Human sperm, 3NP GAM


title: HUG1_HGNP007, Human sperm, 3NP GAM


title: HUG1_HGNP003, Human sperm, 3NP GAM


title: SAR_PFD

city: Palo Alto

first: Sangkyun

last: Cho

state: California

address: 240 Pasteur Dr, Room 3200, Biomedical Innovations Building

institute: Stanford University

description:
Library name: SC_28
10X Genomics snRNAseq (GEX samples from scMultiome)

library_source: transcriptomic single cell

data_processing:
Demultiplexing, barcoded processing, gene counting and aggregation were performed using the standard CellRanger pipeline.
Assembly: hg38 (human) and mm10 (mouse)
Supplementary files format and content: CellRanger output files: barcodes.tsv.gz, features.tsv.gz, matrix.mtx.gz

molecule: nuclear RNA

source_name: Heart (apex)

extract_protocol:
In vitro samples (scRNA-seq): cultured cells were dissociated using accutase for 3 min (STEMCELL Technologies), washed with 1X DPBS (Gibco), strainer-filtered (40 and 70 µm Flowmi) to remove debris, and re-suspended in 0.04% BSA. In vivo samples (snRNA-seq): snap-frozen apical tissue slices were cut into smaller pieces (< 1 mm^3) on a pre-cooled surface on ice using a clean cold razor, and gently homogenized over two rounds using a Dounce homogenizer. The isolated nuclei were then strainer-filtered (40 and 70 µm Flowmi) to remove debris and re-suspended in Diluted Nuclei Buffer (10X Genomics).
After single-cell/nucleus isolation, libraries were constructed following exactly the instructions in the User Guides provided by the manufacturer: 10X Chromium Single Cell 3' v3.1 protocol for the in vitro samples, and 10X Chromium Single Cell Multiome ATAC + GEX (the snRNA-seq GEX portion only) for the in vivo samples.

value: Heart (apex)

value: Sham or TAC (4 weeks) followed by drug treatments (2 weeks)


title: HUG1_HGNP006, Human sperm, 3NP GAM


title: PFD

description:
Library name: SC_27
10X Genomics snRNAseq (GEX samples from scMultiome)


title: HUG1_HGNP004, Human sperm, 3NP GAM


title: HUG1_HGNP001, Human sperm, 3NP GAM


title: SAR

description:
Library name: SC_26
10X Genomics snRNAseq (GEX samples from scMultiome)


title: HUG1_HGNP002, Human sperm, 3NP GAM


title: HUG1_HGNP021, Human sperm, 3NP GAM


title: TAC

description:
Library name: SC_17
10X Genomics snRNAseq (GEX samples from scMultiome)


title: TAM-A33

city: Rennes

first: Raphaël

last: Métivier

address: Campus de Beaulieu

institute: CNRS UMR 6290

data_processing:
Sequencing was carried out on Paired End 100b reads of Illumina NovaSeq.Image analysis and base calling is performed using Illumina Real Time Analysis (3.4.4) with default parameters.  Fastq were generated with bcl2fastq (2.17).
The reads were first trimmed for adapters and low-quality ends by the TrimGalore! Algorithm (-- t, -q, -e, -- length 20) available on the Galaxy web server (https://usegalaxy.org/). Reads were then aligned onto the mouse genome (mm10) by bowtie2 (-- fr, --no-mixed, --no-discordant, -- sensitive)
.bam files containing aligned reads were further processed by the featureCounts tool (-B, -C, -O) (Lio et al., 2013) to evaluate gene expression levels using the mus_musculus. GRCm38.98 Ensembl version of genes’ annotations was used.
Assembly: mm10
Supplementary files format and content: tab-delimited text files that includes  the number of reads aligned on Ensembl GRCm38.98 annotated genes.

source_name: descending thoracic aorta

extract_protocol:
RNA was isolated from descending thoracic aortaes through the phenol-chloroform method using TRIzol (Ambion) reagent. The resulting RNA quality was determined using a Fragment Analyzer Instrument and all samples had RNA integrity numbers above 7.4.
RNA sequencing libraries were prepared according to Illumina's protocols using the Illumina Illumina TruSeq Stranded mRNA kit (ref #20020595). Eleven cycles of PCR were applied to amplify the libraries. Library quality was assessed using a Fragment Analyzer Instrument and libraries were quantified by QPCR using the kit KAPA KK4824, on the QuantStudio 6 of Thermo Fisher Scientific. Sequencing was performed in paired-end (2x150 pb) on an Illumina NovaSeq sequencer at the Integragen company platform

tag: strain

value: Tamoxifen

value: descending thoracic aorta

tag: age

value: 9 weeks


title: HS1042, Human sperm, scHi-C

data_processing:
Hi-C reads of mouse (sperm, mESC) and human (sperm) single-cell samples were mapped to GRCm38 and GRCh38 reference genome separately using BWA-mem29. Contacts were extract with “hickit” package (https://github.com/lh3/hickit, version r291). Contact ends with more than 10 other contact ends within 1 Kb were regard as promiscuous and removed with custom scripts. Contacts with less than 5 other contacts in their 10Mb L0.5 neighborhood were regard as isolated contacts and also removed.
For all cell types, samples with more than 30,000 remaining contacts were kept for downstream analysis. For diploid samples like mESCs, haplotype of contacts was phased according to SNP sites and imputed. For each sample, each chromosome is treated as a polymer chain, each chromosome is evenly divided into 20kb sections, each section is treated as a particle of the polymer chain, and contacts of the sample were used as restraints to search for the optimal x, y, z spatial coordinates of particles by the Force-Directed Graph (FDG) algorithm implemented by “hickit”. 5 models with different random initiate seeds were generated simultaneously and 3 of these models with lowest mean mutual root mean square deviation (RMSD) were selected candidates. The mean mutual RMSD of these 3 models is defined as RMSD of the sample. Samples with RMSD larger than 2 were excluded from structure-based analysis. For samples passed quality control, we randomly picked 1 model from 3 candidates to represent their 3D genome structures.
Assembly: GRCh38, GRCm38
Supplementary files format and content: 3dg file: each line sequentially listing the chromosome name, start position, x, y, and z coordinates, delimited by tab characters
Supplementary files format and content: pairs file: A tab-separated file recording contacts that conforms to 4DN Consortium standards

extract_protocol:
All animal experimental procedures were carried out following the Institutional Animal Care and Use Committee (IACUC) guidelines of Peking University. 10–12-week-old C57BL/6J male mice were purchased from Peking University Laboratory Animal Centre.
The single-cell preamplification product was diluted with water to 5 ng/uL. 2 μL of each diluted sample was transposed by the addition of 3 μL Transposition Mixture (1 μL 5 X TTBL, 0.25 μL TTE Mix V50 (Vazyme TD501), 1.75 μL water) and incubated at 55 °C for 10 min. Transposition was stopped by adding 1.25 μL 0.2% SDS and then the plate was incubated at room temperature for 10 min. Then, 2 μL 5 μM i5 unique dual (UD) index and 2 μL 5 μM i7 unique dual (UD) index were added in each well. Next, the sperm Hi-C library was amplified for 10 cycles of PCR with 9.75 uL KAPA PCR mix (Roche, KK2102) (4 uL 5× KAPA HiFi GC Buffer, 0.6 uL dNTP Mix (10 mM each), 4 uL KAPA HiFi DNA Polymerase (1 U/uL) and 4.75 uL water). The barcoded single-cell libraries were then pooled and purified with 0.6× and 0.15× VAHTS DNA Clean Beads (Vazyme, N411-02). The final libraries were sequenced with paired-end 150bp reads on a NovaSeq X plus (Illumina) platform according to the manufacturer’s instructions.


title: HS1039,HS1040,HS1041, Human sperm, scHi-C


title: TAM-A32


title: TAM-A14


title: HUG1_HGNP008, Human sperm, 3NP GAM


title: HUG1_HGNP011, Human sperm, 3NP GAM


title: TAM-A13


title: HS1027,HS1028,HS1029, Human sperm, scHi-C


title: HUG1_HGNP005, Human sperm, 3NP GAM


title: TAM-A31


title: E4-A40

value: Estetrol


title: TAM-A11


title: E4-A39


title: Sham

description:
Library name: SC_25
10X Genomics snRNAseq (GEX samples from scMultiome)


title: HS1021,HS1022,HS1023, Human sperm, scHi-C


title: HS1024,HS1025,HS1026, Human sperm, scHi-C


title: E4-A38


title: E4-A20


title: HS1018,HS1019,HS1020, Human sperm, scHi-C


title: HS1015,HS1016,HS1017, Human sperm, scHi-C


title: HS1012,HS1013,HS1014, Human sperm, scHi-C


title: E4-A18


title: E4-A16


title: HS1009,HS1010,HS1011, Human sperm, scHi-C


title: HS1006,HS1007,HS1008, Human sperm, scHi-C


title: HS1003,HS1004,HS1005, Human sperm, scHi-C


title: E2-A29

value: Estradiol


title: HS1036,HS1037,HS1038, Human sperm, scHi-C


title: HS1033,HS1034,HS1035, Human sperm, scHi-C


title: HS1030,HS1031,HS1032, Human sperm, scHi-C


title: E2-A26


title: HS1000,HS1001,HS1002, Human sperm, scHi-C


title: HS997,HS998,HS999, Human sperm, scHi-C


title: E2-A10


title: HS994,HS995,HS996, Human sperm, scHi-C


title: HS991,HS992,HS993, Human sperm, scHi-C


title: E2-A6


title: OVX-A25

value: Vehicle


title: HS988,HS989,HS990, Human sperm, scHi-C


title: OVX-A21


title: HS985,HS986,HS987, Human sperm, scHi-C


title: HS982,HS983,HS984, Human sperm, scHi-C


title: HS979,HS980,HS981, Human sperm, scHi-C


title: OVX-A3


title: HS976,HS977,HS978, Human sperm, scHi-C


title: HS973,HS974,HS975, Human sperm, scHi-C


title: OVX-A2


title: HS970,HS971,HS972, Human sperm, scHi-C


title: ESCOBulk2, mESC, Rep2, bulk_Hi-C

data_processing:
Bulk Hi-C reads were processed using the distiller-nf workflow (https://github.com/open2c/distiller-nf). In brief, Hi-C reads were first aligned to the GRCm38 reference genome using the “mem” command of BWA7 (version 0.7.17). Pairtools8 (version 1.0.2) was then used to remove PCR duplicates and extract contacts. Only pairs with both ends having a MAPQ score of >=30 were retained. The resulting contacts file was transformed into a contact matrix and stored in cool format using cooler (version 0.9.3) 9. Iterative correction was applied using the “cooler balance” command to suppress biases in the data.
Assembly: GRCm38
Supplementary files format and content: Contact matrices stored in the cooler format at different resolutions.

extract_protocol:
Bulk Hi-C library preparation in alignment with the preliminary processing steps of the single sperm cell Hi-C protocol, there are deviations in the subsequent procedures post-digestion. After inactivating MboI and cooling to room temperature. The sample was filled in the restriction fragment overhangs and mark the DNA ended with biotin, 20 μL of fill-in master mix (6 μL 1 mM Biotin-14-dATP (Jena Bioscience, NU-835-BIO14-S), 1.8 μL 10 mM dCTP/dGTP/dTTP mix, 4 μl 5 U μL−1 DNA polymerase I, large (Klenow) (NEB, M0210) and 8.2 μL water) was added. Samples were mixed by pipetting and incubated at 37 °C for 90 minutes. 72 μL ligation mix buffer (18 μL 10× T4 DNA ligase buffer, 1 uL 20 mg/mL BSA, 12 μL 1 U/uL T4 DNA ligase, and 41 μL water) was added and samples were incubated at 16 °C for 10 hours.
After ligation, the tube was centrifuged at 2,500 g for 5 minutes at 4 °C, then the supernatant was removed and the pellet was resuspended in 200 μL reverse cross-link buffer (2 μL 1 M Tris pH 8.0, 4 μL 5 M NaCl, 2 μL 60 mg/mL Qiagen Protease, 0.4 μL 0.5M EDTA and 191.6 μL water) and incubated at 50 °C for 1 hour and 70 °C for 30 minutes. The sample was purified with DNA Clean & Concentrator-5 (ZYMO, D4014). To fragment the DNA ligation products, 50 ng of sample DNA was subjected to tagmentation using the TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme, TD501), adhering to the manufacturer's protocol. Notably, only half of the enzyme quantity was utilized in the process and amplified only for 2 cycles. The sample underwent purification and subsequent elution with 20 μL of nuclease-free water. To ascertain the quantity of MyOne Streptavidin beads required for the library pull-down, a ratio of 2 μL MyOne Streptavidin C1 beads per 1 μg of Hi-C DNA was employed, with a minimum threshold set at 10 μL of MyOne Streptavidin C1 beads (Invitrogen, 65001). Here, we took 10 μL of magnetic beads for biotin pull-down. The washing of magnetic beads was performed according to the manufacturer's instructions. After the washing step, the beads were resuspended in 20 μL 2× Binding and Washing (B&W) buffer and mixed with 20 μL ligation products. The tube was incubated at room temperature for 15 minutes, then placed on a magnet for 3 minutes, and discarded the supernatant. Following two rounds of washing with 45 μL of 1× B&W buffer, the beads were subsequently resuspended in a 50 μL PCR reaction mixture (10 μL 5× KAPA HiFi GC Buffer, 1.5 μL dNTP Mix (10 mM each), 1 μL KAPA HiFi DNA Polymerase (1 U/μL), 5 μL N5XX primer, 5 μL N7XX primer (Vazyme, TD202) and 27.5 μL water) and subjected to amplification through a 10-cycle PCR protocol. The barcoded Hi-C library was then pooled and purified with 0.6× and 0.15× VAHTS DNA Clean Beads.


title: E2-A28


title: ESCOBulk1, mESC, Rep1, bulk_Hi-C


title: HS967,HS968,HS969, Human sperm, scHi-C


title: HS964,HS965,HS966, Human sperm, scHi-C


title: E2-A7


title: ESCO1004,ESCO1005,ESCO1006, mESC, scHi-C

source_name: mESC

value: mESC


title: HS961,HS962,HS963, Human sperm, scHi-C


title: HS958,HS959,HS960, Human sperm, scHi-C


title: ESCO1001,ESCO1002,ESCO1003, mESC, scHi-C


title: HS952,HS953,HS954, Human sperm, scHi-C


title: ESCO1102,ESCO1103,ESCO1104, mESC, scHi-C


title: HS955,HS956,HS957, Human sperm, scHi-C


title: ESCO1096,ESCO1097,ESCO1098, mESC, scHi-C


title: OVX-A22


title: ESCO1099,ESCO1100,ESCO1101, mESC, scHi-C


title: ESCO1093,ESCO1094,ESCO1095, mESC, scHi-C


title: HS946,HS947,HS948, Human sperm, scHi-C


title: OVX-A5


title: HS940,HS941,HS942, Human sperm, scHi-C


title: HS943,HS944,HS945, Human sperm, scHi-C


title: ESCO1090,ESCO1091,ESCO1092, mESC, scHi-C


title: ESCO1084,ESCO1085,ESCO1086, mESC, scHi-C


title: ESCO1087,ESCO1088,ESCO1089, mESC, scHi-C


title: HS934,HS935,HS936, Human sperm, scHi-C


title: HS937,HS938,HS939, Human sperm, scHi-C


title: ESCO1078,ESCO1079,ESCO1080, mESC, scHi-C


title: ESCO1007,ESCO1008, mESC, scHi-C


title: HS928,HS929,HS930, Human sperm, scHi-C


title: ESCO1081,ESCO1082,ESCO1083, mESC, scHi-C


title: HS931,HS932,HS933, Human sperm, scHi-C


title: HS925,HS926,HS927, Human sperm, scHi-C


title: ESCO1072,ESCO1073,ESCO1074, mESC, scHi-C


title: ESCO1075,ESCO1076,ESCO1077, mESC, scHi-C


title: HS922,HS923,HS924, Human sperm, scHi-C


title: ESCO1069,ESCO1070,ESCO1071, mESC, scHi-C


title: HS919,HS920,HS921, Human sperm, scHi-C


title: HS949,HS950,HS951, Human sperm, scHi-C


title: ESCO1066,ESCO1067,ESCO1068, mESC, scHi-C


title: HS916,HS917,HS918, Human sperm, scHi-C


title: ESCO1063,ESCO1064,ESCO1065, mESC, scHi-C


title: ESCO1060,ESCO1061,ESCO1062, mESC, scHi-C


title: HS910,HS911,HS912, Human sperm, scHi-C


title: ESCO1057,ESCO1058,ESCO1059, mESC, scHi-C


title: HS907,HS908,HS909, Human sperm, scHi-C


title: HS904,HS905,HS906, Human sperm, scHi-C


title: ESCO1054,ESCO1055,ESCO1056, mESC, scHi-C


title: ESCO1051,ESCO1052,ESCO1053, mESC, scHi-C


title: ESCO1048,ESCO1049,ESCO1050, mESC, scHi-C


title: HS901,HS902,HS903, Human sperm, scHi-C


title: ESCO1045,ESCO1046,ESCO1047, mESC, scHi-C


title: HS892,HS893,HS894, Human sperm, scHi-C


title: ESCO1036,ESCO1037,ESCO1038, mESC, scHi-C


title: HS889,HS890,HS891, Human sperm, scHi-C


title: ESCO1033,ESCO1034,ESCO1035, mESC, scHi-C


title: HS886,HS887,HS888, Human sperm, scHi-C


title: ESCO1030,ESCO1031,ESCO1032, mESC, scHi-C


title: ESCO1042,ESCO1043,ESCO1044, mESC, scHi-C


title: ESCO1039,ESCO1040,ESCO1041, mESC, scHi-C


title: HS895,HS896,HS897, Human sperm, scHi-C


title: HS880,HS881,HS882, Human sperm, scHi-C


title: HS913,HS914,HS915, Human sperm, scHi-C


title: ESCO1027,ESCO1028,ESCO1029, mESC, scHi-C


title: HS877,HS878,HS879, Human sperm, scHi-C


title: ESCO1024,ESCO1025,ESCO1026, mESC, scHi-C


title: HS868,HS869,HS870, Human sperm, scHi-C


title: HS883,HS884,HS885, Human sperm, scHi-C


title: ESCO1021,ESCO1022,ESCO1023, mESC, scHi-C


title: ESCO1018,ESCO1019,ESCO1020, mESC, scHi-C


title: HS874,HS875,HS876, Human sperm, scHi-C


title: ESCO1012,ESCO1013,ESCO1014, mESC, scHi-C


title: HS865,HS866,HS867, Human sperm, scHi-C


title: ESCO1015,ESCO1016,ESCO1017, mESC, scHi-C


title: HS898,HS899,HS900, Human sperm, scHi-C


title: HS862,HS863,HS864, Human sperm, scHi-C


title: HS859,HS860,HS861, Human sperm, scHi-C


title: HS856,HS857,HS858, Human sperm, scHi-C


title: BJ9512, Mouse sperm, scHi-C


title: BJ9509,BJ9510,BJ9511, Mouse sperm, scHi-C


title: BJ9506,BJ9507,BJ9508, Mouse sperm, scHi-C


title: BJ9503,BJ9504,BJ9505, Mouse sperm, scHi-C


title: BJ9500,BJ9501,BJ9502, Mouse sperm, scHi-C


title: HS850,HS851,HS852, Human sperm, scHi-C


title: HS847,HS848,HS849, Human sperm, scHi-C


title: HS871,HS872,HS873, Human sperm, scHi-C


title: HS844,HS845,HS846, Human sperm, scHi-C


title: HS841,HS842,HS843, Human sperm, scHi-C


title: BJ9497,BJ9498,BJ9499, Mouse sperm, scHi-C


title: HS835,HS836,HS837, Human sperm, scHi-C


title: BJ9488,BJ9489,BJ9490, Mouse sperm, scHi-C


title: BJ9494,BJ9495,BJ9496, Mouse sperm, scHi-C


title: BJ9491,BJ9492,BJ9493, Mouse sperm, scHi-C


title: HS829,HS830,HS831, Human sperm, scHi-C


title: BJ9485,BJ9486,BJ9487, Mouse sperm, scHi-C


title: BJ9482,BJ9483,BJ9484, Mouse sperm, scHi-C


title: HS838,HS839,HS840, Human sperm, scHi-C


title: HS832,HS833,HS834, Human sperm, scHi-C


title: ESCO1009,ESCO1010,ESCO1011, mESC, scHi-C


title: HS826,HS827,HS828, Human sperm, scHi-C


title: BJ9476,BJ9477,BJ9478, Mouse sperm, scHi-C


title: HS820,HS821,HS822, Human sperm, scHi-C


title: BJ9479,BJ9480,BJ9481, Mouse sperm, scHi-C


title: HS823,HS824,HS825, Human sperm, scHi-C


title: BJ9473,BJ9474,BJ9475, Mouse sperm, scHi-C


title: HS853,HS854,HS855, Human sperm, scHi-C


title: HS817,HS818,HS819, Human sperm, scHi-C


title: BJ9470,BJ9471,BJ9472, Mouse sperm, scHi-C


title: BJ9467,BJ9468,BJ9469, Mouse sperm, scHi-C


title: HS814,HS815,HS816, Human sperm, scHi-C


title: BJ9464,BJ9465,BJ9466, Mouse sperm, scHi-C


title: HS811,HS812,HS813, Human sperm, scHi-C


title: BJ9461,BJ9462,BJ9463, Mouse sperm, scHi-C


title: HS808,HS809,HS810, Human sperm, scHi-C


title: BJ9458,BJ9459,BJ9460, Mouse sperm, scHi-C


title: BJ9455,BJ9456,BJ9457, Mouse sperm, scHi-C


title: HS802,HS803,HS804, Human sperm, scHi-C


title: HS799,HS800,HS801, Human sperm, scHi-C


title: HS805,HS806,HS807, Human sperm, scHi-C


title: BJ9449,BJ9450,BJ9451, Mouse sperm, scHi-C


title: HS796,HS797,HS798, Human sperm, scHi-C