title: Shoot apex, RNAi_2

extract_protocol:
RNAi_2(200ug)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Carence_Mg_3

status: Public on Nov 17 2021

type: SRA

city: Gif sur Yvette

first: Véronique

last: BRUNAUD

address: avenue des sciences

country: France

institute: INRAE

library_source: transcriptomic

data_processing:
Genome_build: genome of Brassica napus
genome mapping and counts of reads by gene locus are done with STAR; exact multihits are removed and count only consistent PE reads (in PE case).
Supplementary_files_format_and_content: type:count file (txt); content:GeneID,counts_noStranded,counts_forwardStrand,counts_reverseStrand

molecule: total RNA

organism: Brassica napus

growth_protocol: Media : control nutrient solution Hygrometry : hydroponics Temperature : 25°C day / 20°C night Light : 350 μmol m-2 s-1

extract_protocol:
Carence_Mg_3(200mg)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf

treatment_protocol: Mg nutriment deprivation-nutriment deprivation,environmental treatment-nutriment deprivation,abiotic stress-magnesium This experiment used oilseed rape (Brassica napus cv. Trezzor) grown in hydroponic conditions in a greenhouse (20°C day / 15°C night) at the University de Caen-Normandie (France) between February-March. Seeds were germinated on perlite over demineralized water for five days in the dark and then placed two days under natural light. After the first leaf appearance (BBCH 11 stage), ten seedlings were transferred par 10L plastic container (400 x 300 x 115 mm) with natural light supplemented with high-pressure sodium lamps (HPS 400 Watt, Hortilux Schréder, Monster, Netherlands) at 350 μmol m-2 s-1 of photosynthetically active radiation at the canopy height under 16h day / 8h night photoperiod. After 24 days of growth with complete nutrient solution, plants were separated in 9 subsets. Control plants were kept with the same nutrient solution. Each of the others subsets received a specific solution deprived of a unique element: N, Mg, P, S, K, Ca, Cl, Mn, or Co.

tag: genotype

value: wild type (Trezzor)

tag: developmental stage

tag: tissue

value: roots


title: Carence_P_1

extract_protocol:
Carence_P_1(200mg)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf

treatment_protocol: P nutriment deprivation-nutriment deprivation,environmental treatment-nutriment deprivation,abiotic stress-phosphore This experiment used oilseed rape (Brassica napus cv. Trezzor) grown in hydroponic conditions in a greenhouse (20°C day / 15°C night) at the University de Caen-Normandie (France) between February-March. Seeds were germinated on perlite over demineralized water for five days in the dark and then placed two days under natural light. After the first leaf appearance (BBCH 11 stage), ten seedlings were transferred par 10L plastic container (400 x 300 x 115 mm) with natural light supplemented with high-pressure sodium lamps (HPS 400 Watt, Hortilux Schréder, Monster, Netherlands) at 350 μmol m-2 s-1 of photosynthetically active radiation at the canopy height under 16h day / 8h night photoperiod. After 24 days of growth with complete nutrient solution, plants were separated in 9 subsets. Control plants were kept with the same nutrient solution. Each of the others subsets received a specific solution deprived of a unique element: N, Mg, P, S, K, Ca, Cl, Mn, or Co.


title: Carence_Mg_1

extract_protocol:
Carence_Mg_1(200mg)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Carence_N_2

extract_protocol:
Carence_N_2(200mg)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf

treatment_protocol: N nutriment deprivation-nutriment deprivation,environmental treatment-nutriment deprivation,abiotic stress-nitrogen This experiment used oilseed rape (Brassica napus cv. Trezzor) grown in hydroponic conditions in a greenhouse (20°C day / 15°C night) at the University de Caen-Normandie (France) between February-March. Seeds were germinated on perlite over demineralized water for five days in the dark and then placed two days under natural light. After the first leaf appearance (BBCH 11 stage), ten seedlings were transferred par 10L plastic container (400 x 300 x 115 mm) with natural light supplemented with high-pressure sodium lamps (HPS 400 Watt, Hortilux Schréder, Monster, Netherlands) at 350 μmol m-2 s-1 of photosynthetically active radiation at the canopy height under 16h day / 8h night photoperiod. After 24 days of growth with complete nutrient solution, plants were separated in 9 subsets. Control plants were kept with the same nutrient solution. Each of the others subsets received a specific solution deprived of a unique element: N, Mg, P, S, K, Ca, Cl, Mn, or Co.


title: Carence_K_1

extract_protocol:
Carence_K_1(200mg)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf

treatment_protocol: K nutriment deprivation-nutriment deprivation,environmental treatment-nutriment deprivation,abiotic stress-potassium This experiment used oilseed rape (Brassica napus cv. Trezzor) grown in hydroponic conditions in a greenhouse (20°C day / 15°C night) at the University de Caen-Normandie (France) between February-March. Seeds were germinated on perlite over demineralized water for five days in the dark and then placed two days under natural light. After the first leaf appearance (BBCH 11 stage), ten seedlings were transferred par 10L plastic container (400 x 300 x 115 mm) with natural light supplemented with high-pressure sodium lamps (HPS 400 Watt, Hortilux Schréder, Monster, Netherlands) at 350 μmol m-2 s-1 of photosynthetically active radiation at the canopy height under 16h day / 8h night photoperiod. After 24 days of growth with complete nutrient solution, plants were separated in 9 subsets. Control plants were kept with the same nutrient solution. Each of the others subsets received a specific solution deprived of a unique element: N, Mg, P, S, K, Ca, Cl, Mn, or Co.


title: Control_10days_3

extract_protocol:
Control_10days_3(200mg)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf

treatment_protocol: Control_ no nutriment deprivation-no nutriment deprivation,environmental treatment-no nutriment deprivation,abiotic stress-no nutriment deprivation This experiment used oilseed rape (Brassica napus cv. Trezzor) grown in hydroponic conditions in a greenhouse (20°C day / 15°C night) at the University de Caen-Normandie (France) between February-March. Seeds were germinated on perlite over demineralized water for five days in the dark and then placed two days under natural light. After the first leaf appearance (BBCH 11 stage), ten seedlings were transferred par 10L plastic container (400 x 300 x 115 mm) with natural light supplemented with high-pressure sodium lamps (HPS 400 Watt, Hortilux Schréder, Monster, Netherlands) at 350 μmol m-2 s-1 of photosynthetically active radiation at the canopy height under 16h day / 8h night photoperiod. After 24 days of growth with complete nutrient solution, plants were separated in 9 subsets. Control plants were kept with the same nutrient solution. Each of the others subsets received a specific solution deprived of a unique element: N, Mg, P, S, K, Ca, Cl, Mn, or Co.


title: Control_10days_2

extract_protocol:
Control_10days_2(200mg)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Carence_Ca_2

extract_protocol:
Carence_Ca_2(200mg)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf

treatment_protocol: Ca nutriment deprivation-nutriment deprivation,environmental treatment-nutriment deprivation,abiotic stress-calcium This experiment used oilseed rape (Brassica napus cv. Trezzor) grown in hydroponic conditions in a greenhouse (20°C day / 15°C night) at the University de Caen-Normandie (France) between February-March. Seeds were germinated on perlite over demineralized water for five days in the dark and then placed two days under natural light. After the first leaf appearance (BBCH 11 stage), ten seedlings were transferred par 10L plastic container (400 x 300 x 115 mm) with natural light supplemented with high-pressure sodium lamps (HPS 400 Watt, Hortilux Schréder, Monster, Netherlands) at 350 μmol m-2 s-1 of photosynthetically active radiation at the canopy height under 16h day / 8h night photoperiod. After 24 days of growth with complete nutrient solution, plants were separated in 9 subsets. Control plants were kept with the same nutrient solution. Each of the others subsets received a specific solution deprived of a unique element: N, Mg, P, S, K, Ca, Cl, Mn, or Co.


title: Carence_Ca_3

extract_protocol:
Carence_Ca_3(200mg)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Control_10days_1

extract_protocol:
Control_10days_1(200mg)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Carence_Ca_1

extract_protocol:
Carence_Ca_1(200mg)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Week3 - rep1

status: Public on Nov 08 2021

city: Memphis

first: Tian

last: Mi

state: TN

address: 262 Danny Thomas Pl MS351

department: Immunology

country: USA

institute: St. Jude Children's Research Hospital

description: GMP: Good Manufacturing Practice (cells have chimeric antigen vector added following drug production practices).

library_source: genomic

data_processing:
Basecalling was performed using Illumina Casava 1.7.
Sequencing reads were aligned to the HG19 genome using the BSMAP v.2.74 software.
CpG methylation levels were determined by methratio.py script from BSMAP.
Genome_build: hg19 (GRCh37)
Supplementary_files_format_and_content: *.txt: Tab-delimited text files with columns of chr, loc, cov, meth.

molecule: genomic DNA

organism: Homo sapiens

source_name: CD19-CAR T cells

extract_protocol:
DNA was extracted from the sorted cells by using a DNA-extraction kit (Qiagen) and then bisulfite treated using an EZ DNA methylation kit (Zymo Research).
Bisulfite-modified DNA sequencing libraries were generated using the EpiGenome kit (Epicentre) according to the manufacturer’s instructions. Bisulfite-modified DNA libraries were sequenced using NovaSeq 6000 system.

tag: disease

value: B-cell acute lymphoblastic leukemia

tag: cell type

value: CD8+ T cells

tag: time point

value: Week 3


title: Week4 - rep1

value: Week 4


title: Week2 - rep5

value: Week 2


title: Week3 - rep2


title: Week2 - rep4


title: Week2 - rep3


title: Carence_N_1

extract_protocol:
Carence_N_1(200mg)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Week1 - rep4

value: Week 1


title: Week2 - rep2


title: Week2 - rep1


title: Carence_Mg_2

extract_protocol:
Carence_Mg_2(200mg)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Week1 - rep2


title: Carence_N_3

extract_protocol:
Carence_N_3(200mg)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Week1 - rep3


title: Week1 - rep1


title: Carence_K_2

extract_protocol:
Carence_K_2(200mg)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: GMP - rep13

value: Week 0


title: Carence_K_3

extract_protocol:
Carence_K_3(200mg)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: GMP - rep9


title: GMP - rep10


title: GMP - rep8


title: GMP - rep12


title: GMP - rep11


title: GMP - rep6


title: GMP - rep7


title: GMP - rep5


title: GMP - rep3


title: GMP - rep4


title: GMP - rep2


title: Week3 - rep3


title: Pre-infusion - rep4

value: Before Week 0


title: Pre-infusion - rep3


title: Pre-infusion - rep2


title: Pre-infusion - rep1


title: 0XE2_on [0XE2.0Q]

status: Public on Aug 13 2021

city: Lausanne

first: David

last: Barras

state: Vaud

address: AGORA, Bugnon 25A

department: Oncology

country: Switzerland

institute: Centre hospitalier universitaire vaudois

data_processing:
Sequencing data were demultiplexed using the bcl2fastq2Conversion Software (v. 2.20, Illumina).
Tumor RNA sequencing processingIllumina single-end sequencing reads were aligned to the human reference GRCh37.75 genome using STARaligner and the 2-pass method as briefly followed: the reads were aligned in a first round using the --runMode alignReadsparameter, then a sample-specific splice-junction index was created using the --runMode genomeGenerateparameter.
Finally, the reads were aligned using this newly created index as a reference.The number of counts was summarized at the gene level using htseq-count
Genome_build: GRCh37
Supplementary_files_format_and_content: Processed coding gene expression log2(RPKM+1) with Gene Symbol mapping

extract_protocol:
RNA extraction and library preparation RNA from snap frozen tissue biopsies was extracted using the RNA easy kit (Qiagen, Hilden, Germany). RNA quality was assessed using the Fragment Analyzer (Advances Analytical Technologies Inc., Ankeny, USA).
RNA-seq libraries were prepared from 250 ng oftotal RNA. The RNA samples were depleted for ribosomal RNAs with the Illumina TruSeq Stranded Total RNA Goldkit. Ribosomal-RNA depleted RNA was then converted in cDNA and amplified with the kit NugenOvation®RNA-Seq System V2. It was used to generate sequencing libraries with the Illumina TruSeq DNA Nano kit.
Libraries were quantified by a fluorimetric method and their quality assessed on a Fragment Analyzer (Agilent Technologies). Cluster generationwas performed with 2 nM of an equimolar pool from the resulting libraries using the Illumina HiSeq 3000/4000 SR Cluster Kit reagents and sequenced on the Illumina HiSeq 4000 using HiSeq 3000/4000 SBS Kit reagents for 150 cycles.

tag: patient

tag: treatment

value: PD

value: tumors


title: 0XE2_pre [0XE2.05]


title: 12UI_on [12UI.1B]

value: Ovarian

value: SD


title: 1EEY_on [1EEY.1G]

value: Prostate

value: PR


title: 095T_on [095T.0Y]


title: 1EEY_pre [1EEY.0Q]


title: 02F5_on [02F5.16]


title: 02F5_pre [02F5.0J]


title: 095T_pre [095T.0C]


title: 0XUL_pre [0XUL.0D]


title: 12UI_pre [12UI.0L]


title: 0XUL_on [0XUL.0Y]


title: 19F7_on [19F7.0R]

value: Gallbladder


title: GMP - rep1


title: Rep1_KKT2_input

status: Public on Aug 02 2021

city: Edinburgh

first: Robin

last: Allshire

address: Max Born Crescent

country: United Kingdom

institute: Wellcome Centre for Cell Biology

data_processing:
Collapsing. Command: pyFastqDuplicateRemover.py -f paired-end_file_1.fastq -r paired-end_file_2.fastq -o collapsed_file
Alignment. Command: bowtie2 -f -x Tbruceibrucei427  -1  collapsed_paired-end_file_1.fasta - 2 collapsed_paired-end_file_2.fasta > aligned_file.sam
Converting from SAM to BAM format. Command: samtools view -bt Tbruceibrucei427.fasta.fai aligned_file.sam -o aligned_file.bam
Sorting. Command: samtools sort aligned_file.bam -o aligned_sorted_file.bam
Indexing.Command: samtools index aligned_sorted_file.bam
Normalization of IP to input and to library size. Command: bamCompare --operation ratio -b1 aligned_sorted_IP_file.bam -b2 aligned_sorted_input_file.bam -o IP_to_input_ratio.bw --outFileFormat bigwig --binSize 50 --normalizeUsing CPM --scaleFactorsMethod None
Genome_build: HGAP3_Tb427v9.2
Supplementary_files_format_and_content: bigWig files contain information about the peak distribution of each protein across the T. brucei genome

organism: Trypanosoma brucei brucei

source_name: cultured cells

growth_protocol: Cell lines were grown at 37 oC and 5% CO2 in HMI-9 medium supplemented with 10% Fetal Calf Serum (Gibco) and 1% Penicillin-Streptomycin (Gibco). Cell lines with tagged proteins were grown in the presence of 5 µg/ml blasticidin.

extract_protocol:
Briefly, 4 x 10^8 parasites were harvested and fixed with 0.8% formaldehyde for 20 min at room temperature. Cells were lysed and sonicated in the presence of 0.2% SDS for 30 cycles (30 s on, 30 s off) using the high setting on a Bioruptor sonicator (Diagenode). Input samples were taken before incubating the rest of the cell lysates overnight with 10 µg rabbit anti-GFP antibody (Thermo Fisher Scientific) and Protein G Dynabeads. The beads were washed and the DNA eluted from them was treated with RNAse and Proteinase K. DNA was purified using a QIAquick PCR Purification Kit (Qiagen).
ChIP-seq libraries were constructed using 0.5-20 ng of immunoprecipitated or input DNA. Following blunt-ending and poly(A)-tailing, NEXTflex (Bioo Scientific) adapters containing barcodes were ligated to each DNA fragment. Fragments were size selected using AMPure beads (Beckman Coulter). The amount of DNA in the resulting libraries was quantified by Qubit fluorometer using Qubit dsDNA HS Assay Kit (Invitrogen). Fragment size distribution was assessed by 2100 Bioanalyzer Instrument (Agilent Technologies) using a Bioanalyzer High Sensitivity DNA Analysis kit (Agilent). Ideally, the libraries had a peak at 300 bp (~ 150 bp fragment DNA + ~ 150 bp adapter sequence). Libraries were sequenced on Illumina MiniSeq, HiSeq or NextSeq. In all cases, 75 bp paired-end sequencing was performed.

tag: strain

value: Lister 427

value: bloodstream slender form

tag: chromatin factor


title: Rep1_KKT2_IP

tag: antibody

value: anti-GFP


title: S100_Aux_3

status: Public on Jun 21 2021

data_processing:
Genome_build: genome of RChinensis_v1 OBDH_1.0
Mapping and counts of reads by locus gene (tophat2/bowtie2). If PE reads, count only consistent PE reads
Supplementary_files_format_and_content: type:count file (txt); content:gene ID,length of Gene,nb single reads,nb PE reads

organism: Rosa hybrid cultivar

growth_protocol: clumps fertilized 54 cells during the rooting period then transferred to a substrate with neutral peat, coconut fibers and perlite up to the pvmt greenhouse: minimum humidity 60%; climatic chamber: 60% greenhouse: Day = 19°C mini aeration at 25°C; climatic chamber: 22°C Greenhouse: Total lighting duration of 16 hours. The lighting is provided by sodium vapor lamps with a power of 400 W. There are 9 lamps for a module of 90 M2. Turning on the lights half an hour before sunrise. Mini lighting for triggering from 100W / M2. Climate chamber: 95micromol, lighting is provided by two fluorescent tubes

extract_protocol:
S100_Aux_3(1ug)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf

value: wild type

tag: dates

value: planting date=20-10-17, harvest date=20-10-17

value: flower bud

value: saccharose 100 mM + NAA 1uM


title: S100_NoAux_3

extract_protocol:
S100_NoAux_3(1ug)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf

value: saccharose 100 mM


title: S100_NoAux_2

extract_protocol:
S100_NoAux_2(1ug)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf

value: planting date=29-08-17, harvest date=20-08-17


title: S10_Aux_3

extract_protocol:
S10_Aux_3(1ug)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf

value: saccharose 10 mM + NAA 1uM


title: S10_NoAux_1

extract_protocol:
S10_NoAux_1(1ug)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf

value: planting date=02-05-17, harvest date=20-06-17

value: saccharose 10 mM


title: S10_Aux_2

extract_protocol:
S10_Aux_2(1ug)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: S10_NoAux_3

extract_protocol:
S10_NoAux_3(1ug)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: S10_NoAux_2

extract_protocol:
S10_NoAux_2(1ug)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: S100_Aux_2

extract_protocol:
S100_Aux_2(1ug)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Rep2_KKT2_input


title: Rep2_KKT2_IP


title: d8-2A RNA-Seq

status: Public on Jun 04 2021

city: Rotterdam

first: Niels

last: Galjart

department: Cell Biology

country: Netherlands

institute: Erasmus University Medical Centre

data_processing:
RNA-Seq : Reads were aligned to the mouse genome (mm10 build) using HISAT2. Sample scaling and statistical analysis were performed using the R package DESeq2.
MedSeq : Raw fastq files were subjected to Illumina adaptor trimming and reads were filtered based on LpnPI restriction site occurrence between 13‐17 bp from either 5’ or 3’ end of the read. Reads that passed the filter were mapped to mm10 using bowtie2.1.0. BAM files were generated using SAMtools for visualization.
Genome_build: mm10
Supplementary_files_format_and_content: bw files were generated from bam files using deepTools (bamCoverage). Bam files were generated from fastq files using different packages.

organism: Mus musculus

source_name: ES cells

extract_protocol:
RNA-Seq : RNA was harvested from ES cells using Trizol reagent.
MedSeq : Genomic DNA was digested with LpnPI.
RNA-Seq samples were prepared with the Illumina TruSeq Stranded mRNA Library Prep Kit. The resulting DNA libraries were sequenced according to the Illumina TruSeq Rapid  v2 protocol on an Illumina HiSeq2500 sequencer, generating single-end reads of 50bp.
MedSeq : LpnPI-digested genomic DNA was processed using the ThruPlex DNA–seq 96D kit (Rubicon Genomics cat#R400407). Stem‐loop adapters were blunt end ligated to repaired input DNA and amplified (4 +10 cycles) to include dual indexed barcodes to yield an indexed Illumina NGS library. Multiplexed samples were sequenced on Illumina HiSeq2500 systems for single read of 50 base pairs.


title: GC1A RNA-Seq

value: GFP-CTCF


title: GC1B RNA-Seq


title: GC1C RNA-Seq


title: d8-1C RNA-Seq


title: d8-2B RNA-Seq


title: GC2B RNA-Seq


title: d8-3B RNA-Seq


title: d8-3A RNA-Seq


title: GC2C RNA-Seq


title: d8-2C RNA-Seq


title: d8-1B RNA-Seq


title: S10_Aux_1

extract_protocol:
S10_Aux_1(1ug)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf

value: planting date=02-06-17, harvest date=20-06-17


title: d8-1A RNA-Seq


title: Tmorph_Stage2_2

status: Public on Jun 03 2021

data_processing:
Genome_build: transcriptome of Nigelle assembly (REFNigelle-afteriAssembler)
transcriptome mapping and counts of reads by gene locus are done with bowtie2; exact multihits are removed and count only consistent PE reads (in PE case).
Supplementary_files_format_and_content: type:count file (txt); content:GeneID,gene_length,counts_SEreads,counts_PEreads

organism: Nigella damascena

growth_protocol: growth chamber: 25° C day for 18h, 16 °C night for 6h

extract_protocol:
Tmorph_Stage2_2(2ug)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf

treatment_protocol: no treatment

tag: Stage

value: wild type (pp)

value: petal


title: Tmorph_Stage2_3

extract_protocol:
Tmorph_Stage2_3(2ug)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: S100_Aux_1

extract_protocol:
S100_Aux_1(1ug)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf

value: planting date=02-08-17, harvest date=20-06-17


title: S100_NoAux_1

extract_protocol:
S100_NoAux_1(1ug)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf

value: planting date=02-07-17, harvest date=20-06-17


title: Tmorph_Stage2_1

extract_protocol:
Tmorph_Stage2_1(2ug)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Tmorph_Stage1_1

extract_protocol:
Tmorph_Stage1_1(2ug)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Pmorph_Stage1_3

extract_protocol:
Pmorph_Stage1_3(2ug)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf

value: wild type (PP)


title: Tmorph_Stage1_2

extract_protocol:
Tmorph_Stage1_2(2ug)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Pmorph_Stage1_2

extract_protocol:
Pmorph_Stage1_2(2ug)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Pmorph_Stage2_1

extract_protocol:
Pmorph_Stage2_1(2ug)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Pmorph_Stage2_3

extract_protocol:
Pmorph_Stage2_3(2ug)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Stage3_3

status: Public on Apr 28 2021

data_processing:
Genome_build: transcriptome of Brachypodium_transcripts
transcriptome mapping and counts of reads by gene locus are done with bowtie2; exact multihits are removed and count only consistent PE reads (in PE case).
Supplementary_files_format_and_content: type:count file (txt); content:GeneID,gene_length,counts_SEreads,counts_PEreads

organism: Brachypodium distachyon

growth_protocol: Media=soil hygrometry=60% Temperature=19 Light=18h light

extract_protocol:
Stage3_3(500pg)
rna_selection:selection polyA, paired-end, stranded:no, Protocol:SMARTerUltraLowInputRNAKitforSequencing-v3UserManual_040214.pdf

value: wild type (Bd21-3)

value: age=19day 3 to 4 cells of protoxylem cells

value: meristem


title: Tmorph_Stage1_3

extract_protocol:
Tmorph_Stage1_3(2ug)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Pmorph_Stage2_2

extract_protocol:
Pmorph_Stage2_2(2ug)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Pmorph_Stage1_1

extract_protocol:
Pmorph_Stage1_1(2ug)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Stage3_2

extract_protocol:
Stage3_2(500pg)
rna_selection:selection polyA, paired-end, stranded:no, Protocol:SMARTerUltraLowInputRNAKitforSequencing-v3UserManual_040214.pdf


title: GC2A RNA-Seq

extract_protocol:
RNA-Seq : RNA was harvested from ES cells using Trizol reagent.
MedSeq : Genomic DNA was digested with LpnPI.
RNA-Seq samples were prepared with the Illumina TruSeq Stranded mRNA Library Prep Kit. The resulting DNA libraries were sequenced according to the Illumina TruSeq Rapid  v2 protocol on an Illumina HiSeq2500 sequencer, generating single-end reads of 50bp.
MedSeq : LpnPI-digested genomic DNA was processed using the ThruPlex DNA–seq 96D kit (Rubicon Genomics cat#R400407). Stem‐loop adapters were blunt end ligated to repaired input DNA and amplified (4 +10 cycles) to include dual indexed barcodes to yield an indexed Illumina NGS library. Multiplexed samples were sequenced on Illumina HiSeq2500 systems for single read of 50 base pairs.


title: Stage2_1

extract_protocol:
Stage2_1(500pg)
rna_selection:selection polyA, paired-end, stranded:no, Protocol:SMARTerUltraLowInputRNAKitforSequencing-v3UserManual_040214.pdf

value: age=19day 1 to 2 cells of protoxylem cells


title: Stage2_3

extract_protocol:
Stage2_3(500pg)
rna_selection:selection polyA, paired-end, stranded:no, Protocol:SMARTerUltraLowInputRNAKitforSequencing-v3UserManual_040214.pdf


title: Control_3

extract_protocol:
Control_3(500pg)
rna_selection:selection polyA, paired-end, stranded:no, Protocol:SMARTerUltraLowInputRNAKitforSequencing-v3UserManual_040214.pdf


title: Stage1_1

extract_protocol:
Stage1_1(500pg)
rna_selection:selection polyA, paired-end, stranded:no, Protocol:SMARTerUltraLowInputRNAKitforSequencing-v3UserManual_040214.pdf

value: age=19day no perceptible protoxylem cell


title: Stage1_2

extract_protocol:
Stage1_2(500pg)
rna_selection:selection polyA, paired-end, stranded:no, Protocol:SMARTerUltraLowInputRNAKitforSequencing-v3UserManual_040214.pdf


title: Stage2_2

extract_protocol:
Stage2_2(500pg)
rna_selection:selection polyA, paired-end, stranded:no, Protocol:SMARTerUltraLowInputRNAKitforSequencing-v3UserManual_040214.pdf


title: Stage1_3

extract_protocol:
Stage1_3(500pg)
rna_selection:selection polyA, paired-end, stranded:no, Protocol:SMARTerUltraLowInputRNAKitforSequencing-v3UserManual_040214.pdf


title: Stage3_1

extract_protocol:
Stage3_1(500pg)
rna_selection:selection polyA, paired-end, stranded:no, Protocol:SMARTerUltraLowInputRNAKitforSequencing-v3UserManual_040214.pdf


title: Control_1

extract_protocol:
Control_1(500pg)
rna_selection:selection polyA, paired-end, stranded:no, Protocol:SMARTerUltraLowInputRNAKitforSequencing-v3UserManual_040214.pdf


title: Control_2

extract_protocol:
Control_2(500pg)
rna_selection:selection polyA, paired-end, stranded:no, Protocol:SMARTerUltraLowInputRNAKitforSequencing-v3UserManual_040214.pdf


title: Col0 2

status: Public on Apr 27 2021

data_processing:
Genome_build: transcriptome of TAIR-R8 mitochondrial genome
transcriptome mapping and counts of reads by gene locus are done with bowtie2; exact multihits are removed and count only consistent PE reads (in PE case).
Supplementary_files_format_and_content: type:count file (txt); content:GeneID,gene_length,counts_SEreads,counts_PEreads

organism: Arabidopsis thaliana

growth_protocol: Plants were grown in a greenhouse in long day condition (soil)

extract_protocol:
Col0_2(2mg)
rna_selection:ribosomal depletion, paired-end, stranded:no, Protocol:TruSeq_RNA_SamplePrep_v2_Guide_15026495_C.pdf

value: wild type (WT)

tag: growth

value: flower bud


title: Col0 1

extract_protocol:
Col0_1(2mg)
rna_selection:ribosomal depletion, paired-end, stranded:no, Protocol:TruSeq_RNA_SamplePrep_v2_Guide_15026495_C.pdf


title: rfl8-3HA_2

extract_protocol:
rfl8-3HA_2(2mg)
rna_selection:ribosomal depletion, paired-end, stranded:no, Protocol:TruSeq_RNA_SamplePrep_v2_Guide_15026495_C.pdf

value: ectopic copy of rfl8 CDS fused in frame with 3-HA tag - mutant=AT1G62720


title: Col0 3

extract_protocol:
Col0_3(2mg)
rna_selection:ribosomal depletion, paired-end, stranded:no, Protocol:TruSeq_RNA_SamplePrep_v2_Guide_15026495_C.pdf


title: rfl8-3HA_1

extract_protocol:
rfl8-3HA_1(2mg)
rna_selection:ribosomal depletion, paired-end, stranded:no, Protocol:TruSeq_RNA_SamplePrep_v2_Guide_15026495_C.pdf


title: rfl8-3HA_3

extract_protocol:
rfl8-3HA_3(2mg)
rna_selection:ribosomal depletion, paired-end, stranded:no, Protocol:TruSeq_RNA_SamplePrep_v2_Guide_15026495_C.pdf


title: rfl8_2

data_processing:
Genome_build: transcriptome of Arabidopsis mitochondrial genome
transcriptome mapping and counts of reads by gene locus are done with bowtie2; exact multihits are removed and count only consistent PE reads (in PE case).
Supplementary_files_format_and_content: type:count file (txt); content:GeneID,gene_name,counts

growth_protocol: in vitro Media=MRPlant Hygrometry=50% Temperature=21°C Light=16h

extract_protocol:
rfl8_2(2.5mg)
rna_selection:ribosomal depletion, single, stranded:no, Protocol:5132-05-NEXTflex-Small-RNA-Seq-v3-16-06.pdf

value: T-DNA mutant rfl8 SALK_0015489 - mutant=knock-out,insertional mutagen,T-DNA-AT1G62720

value: seedling


title: col0 (2)

extract_protocol:
col0_2(2.5mg)
rna_selection:ribosomal depletion, single, stranded:no, Protocol:5132-05-NEXTflex-Small-RNA-Seq-v3-16-06.pdf


title: rfl8_1

extract_protocol:
rfl8_1(2.5mg)
rna_selection:ribosomal depletion, single, stranded:no, Protocol:5132-05-NEXTflex-Small-RNA-Seq-v3-16-06.pdf


title: col0_(2)

data_processing:
Genome_build: genome of Arabidopsis mitochondrial genome
genome mapping and counts of reads by gene locus are done with STAR; exact multihits are removed and count only consistent PE reads (in PE case).
Supplementary_files_format_and_content: type:count file (txt); content:GeneID,counts

growth_protocol: Plants were grown in a greenhouse in soil and in long day condition

extract_protocol:
col0_2(2mg)
rna_selection:ribosomal depletion, single, stranded:no, Protocol:5132-05-NEXTflex-Small-RNA-Seq-v3-16-06.pdf

treatment_protocol: RNAseI-genetic treatment-enzymatic,earthworms-rnaseqi RNaseI treatment of mitochondrial extracts

value: flower


title: col0_(1)

extract_protocol:
col0_1(2mg)
rna_selection:ribosomal depletion, single, stranded:no, Protocol:5132-05-NEXTflex-Small-RNA-Seq-v3-16-06.pdf


title: rfl8-3HA_(1)

growth_protocol: Plants were grown in a greenhouse, in soil, in long day condition

extract_protocol:
rfl8-3HA_1(2mg)
rna_selection:ribosomal depletion, single, stranded:no, Protocol:5132-05-NEXTflex-Small-RNA-Seq-v3-16-06.pdf

value: wild type (rfl8 rfl8 CDS fused in frame with 3-HA tag)


title: col0 (1)

extract_protocol:
col0_1(2.5mg)
rna_selection:ribosomal depletion, single, stranded:no, Protocol:5132-05-NEXTflex-Small-RNA-Seq-v3-16-06.pdf


title: rfl8-3HA_(2)

extract_protocol:
rfl8-3HA_2(2mg)
rna_selection:ribosomal depletion, single, stranded:no, Protocol:5132-05-NEXTflex-Small-RNA-Seq-v3-16-06.pdf


title: B73_V2_LF_WW_2

status: Public on Dec 14 2020

data_processing:
Genome_build: genome of Zea_mays.B73_RefGen_v4.41
genome mapping and counts of reads by gene locus are done with STAR; exact multihits are removed and count only consistent PE reads (in PE case).
Supplementary_files_format_and_content: type:count file (txt); content:GeneID,counts_noStranded,counts_forwardStrand,counts_reverseStrand

organism: Zea mays

growth_protocol: Growth conditions: PhenoArch greenhouse Media : 30:70 (v/v) mixture of clay and organic compost Hygrometry : 60-80% RH (air relative humidity) Temerature : 22 ± 1°C (min: 17°C night, max 27°C day) Light : solar radiation +  400‐W HPS lamps to ensure 14h photoperiod and at least 200 µmol m-2 s-1 PPFD (photosynthetic photon flux density)

extract_protocol:
B73_V2_LF_WW_2(5ng)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf

value: wild type (B73)

value: leaf


title: B73_V2_LF_WW_1

extract_protocol:
B73_V2_LF_WW_1(5ng)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: B73_V2_LF_WW_3

extract_protocol:
B73_V2_LF_WW_3(5ng)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: B73_V2_LF-I_WW_3

extract_protocol:
B73_V2_LF-I_WW_3(5ng)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: B73_V2_LF-I_WW_2

extract_protocol:
B73_V2_LF-I_WW_2(5ng)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: B73_PS_TAS_WW_3

extract_protocol:
B73_PS_TAS_WW_3(5ng)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf

value: aerial


title: B73_V2_LF-I_WW_1

extract_protocol:
B73_V2_LF-I_WW_1(5ng)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: B73_PS_TAS_WW_2

extract_protocol:
B73_PS_TAS_WW_2(5ng)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: B73_PS_LF_WW_2

extract_protocol:
B73_PS_LF_WW_2(5ng)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: B73_PS_SILK_WW_3

extract_protocol:
B73_PS_SILK_WW_3(5ng)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf

value: silk


title: B73_PS_SILK_WW_2

extract_protocol:
B73_PS_SILK_WW_2(5ng)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: B73_PS_TAS_WW_1

extract_protocol:
B73_PS_TAS_WW_1(5ng)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: B73_PS_SILK_WW_1

extract_protocol:
B73_PS_SILK_WW_1(5ng)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: B73_PS_EAR_WW_2

extract_protocol:
B73_PS_EAR_WW_2(5ng)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: B73_PS_EAR_WW_3

extract_protocol:
B73_PS_EAR_WW_3(5ng)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: B73_PS_IN1_WW_3

extract_protocol:
B73_PS_IN1_WW_3(5ng)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf

value: ear internode


title: B73_DAS4_R_WW_2

growth_protocol: Growth conditions: S2 greenhouse Media : Perlite Hygrometry : no control of relative humidity Temerature : 24/19°C (day/night) Light : without direct light

extract_protocol:
B73_DAS4_R_WW_2(5ng)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf

value: roots


title: B73_PS_EAR_WW_1

extract_protocol:
B73_PS_EAR_WW_1(5ng)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: B73_DAS4_HY_WW_3

extract_protocol:
B73_DAS4_HY_WW_3(5ng)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf

value: hypocotyl


title: B73_DAS4_R_WW_1

extract_protocol:
B73_DAS4_R_WW_1(5ng)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: B73_PS_IN1_WW_1

extract_protocol:
B73_PS_IN1_WW_1(5ng)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: B73_DAS4_HY_WW_1

extract_protocol:
B73_DAS4_HY_WW_1(5ng)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: B73_DAP35_S_WW_1

growth_protocol: Growth conditions: Field Media : Field soil Hygrometry : Saint-Martin-de-Hinx (South-West France), Summer 2017 Temerature : Saint-Martin-de-Hinx (South-West France), Summer 2017 Light : Saint-Martin-de-Hinx (South-West France), Summer 2017

extract_protocol:
B73_DAP35_S_WW_1(5ng)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf

value: seed


title: B73_DAS4_R_WW_3

extract_protocol:
B73_DAS4_R_WW_3(5ng)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: B73_PS_IN1_WW_2

extract_protocol:
B73_PS_IN1_WW_2(5ng)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: B73_PS_LF_WW_3

extract_protocol:
B73_PS_LF_WW_3(5ng)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: B73_DAP12_S_WW_1

extract_protocol:
B73_DAP12_S_WW_1(5ng)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: B73_DAS4_HY_WW_2

extract_protocol:
B73_DAS4_HY_WW_2(5ng)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: 6h-NoInf_2mM-NO3_3

status: Public on Oct 22 2020

data_processing:
Genome_build: transcriptome of Arabidopsis TAIR10 and Botrytis B05.1
Mapping and counts of reads by locus gene (tophat2/bowtie2). If PE reads, count only consistent PE reads
Supplementary_files_format_and_content: type:count file (txt); content:gene ID,length of Gene,nb single reads,nb PE reads

growth_protocol: Media = 2 mM Nitrate

extract_protocol:
6h-NoInf_2mM-NO3_3(100mg)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf

treatment_protocol: Name:2mM-NO3 - environmental treatment - nitrogen concentration,botrytis cinerea:quantity 2mM . Plants were grown for 5 weeks on diffrent nitrate concentrations. Rosette leaves were cut and infected (Inf) or not (NoInf) with Botrytis mycelium (B0510 strain) and placed in continuous light for 6 hours and harvested.

value: wild type

value: rosette

tag: media

value: 2 mM Nitrate

tag: continuous light

tag: infection

value: No


title: B73_DAP35_S_WW_2

extract_protocol:
B73_DAP35_S_WW_2(5ng)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf

tag: genotype


title: B73_DAP12_S_WW_3

extract_protocol:
B73_DAP12_S_WW_3(5ng)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf

tag: developmental stage


title: 6h-NoInf_2mM-NO3_1

extract_protocol:
6h-NoInf_2mM-NO3_1(100mg)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf

tag: tissue


title: 6h-NoInf_10mM-NO3_2

growth_protocol: Media = 10 mM Nitrate

extract_protocol:
6h-NoInf_10mM-NO3_2(100mg)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf

treatment_protocol: Name:10mM-NO3 - environmental treatment - nitrogen,nitrogen:quantity 10mM . Plants were grown for 5 weeks on different nitrate concentrations. Rosette leaves were cut and infected (Inf) or not (NoInf) with Botrytis mycelium (B0510 strain) and placed in continuous light for 6 or 10 hours and harvested.

value: 10 mM Nitrate


title: B73_PS_LF_WW_1

extract_protocol:
B73_PS_LF_WW_1(5ng)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: 6h-NoInf_10mM-NO3_3

extract_protocol:
6h-NoInf_10mM-NO3_3(100mg)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: 6h-NoInf_10mM-NO3_1

extract_protocol:
6h-NoInf_10mM-NO3_1(100mg)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: 6h-NoInf_0.5mM-NO3_2

growth_protocol: Media = 0,5 mM Nitrate

extract_protocol:
6h-NoInf_0.5mM-NO3_2(100mg)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf

treatment_protocol: Name:0.5mM-NO3 - environmental treatment - nitrogen,nitrogen:quantity 0.5mM . Plants were grown for 5 weeks on different nitrate concentrations. Rosette leaves were cut and infected (Inf) or not (NoInf) with Botrytis mycelium (B0510 strain) and placed in continuous light for 6 or 10 hours and harvested.

value: 0,5 mM Nitrate


title: 6h-NoInf_0.5mM-NO3_3

extract_protocol:
6h-NoInf_0.5mM-NO3_3(100mg)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: 6h-NoInf_0.5mM-NO3_1

extract_protocol:
6h-NoInf_0.5mM-NO3_1(100mg)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: 6h-Inf_2mM-NO3_1

extract_protocol:
6h-Inf_2mM-NO3_1(100mg)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf

treatment_protocol: Name:6h-Inf_2mM-NO3 - and environmental treatment,pathogen - and nitrogen,pathogen infection,nitrogen_botrytis-mycelium:quantity 2mM time 6hour . Plants were grown for 5 weeks on different nitrate concentrations. Rosette leaves were cut and infected (Inf) or not (NoInf) with Botrytis mycelium (B0510 strain) and placed in continuous light for 6 hours and harvested.

value: Botrytis mycelium (B0510 strain)


title: B73_DAP35_S_WW_3

extract_protocol:
B73_DAP35_S_WW_3(5ng)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: 6h-Inf_10mM-NO3_3

extract_protocol:
6h-Inf_10mM-NO3_3(100mg)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf

treatment_protocol: Name:6h-Inf_10mM-NO3 - and environmental treatment,pathogen - and nitrogen,pathogen infection,nitrogen_botrytis-mycelium:quantity 10mM time 6hour . Plants were grown for 5 weeks on different nitrate concentrations. Rosette leaves were cut and infected (Inf) or not (NoInf) with Botrytis mycelium (B0510 strain) and placed in continuous light for 6 hours and harvested.


title: 6h-Inf_2mM-NO3_3

extract_protocol:
6h-Inf_2mM-NO3_3(100mg)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: 6h-Inf_10mM-NO3_2

extract_protocol:
6h-Inf_10mM-NO3_2(100mg)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: 6h-Inf_10mM-NO3_1

extract_protocol:
6h-Inf_10mM-NO3_1(100mg)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: 6h-Inf_0.5mM-NO3_3

extract_protocol:
6h-Inf_0.5mM-NO3_3(100mg)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf

treatment_protocol: Name:6h-Inf_0.5mM-NO3 - and environmental treatment,pathogen - and nitrogen,pathogen infection,nitrogen_botrytis-mycelium:quantity 0.5mM time 6hour . Plants were grown for 5 weeks on different nitrate concentrations. Rosette leaves were cut and infected (Inf) or not (NoInf) with Botrytis mycelium (B0510 strain) and placed in continuous light for 6 hours and harvested.


title: 6h-NoInf_2mM-NO3_2

extract_protocol:
6h-NoInf_2mM-NO3_2(100mg)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: 10h-NoInf_10mM-NO3_1

extract_protocol:
10h-NoInf_10mM-NO3_1(100mg)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: 10h-NoInf_2mM-NO3_1

extract_protocol:
10h-NoInf_2mM-NO3_1(100mg)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: 10h-Inf_2mM-NO3_1

extract_protocol:
10h-Inf_2mM-NO3_1(100mg)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf

treatment_protocol: Name:10h-Inf_2mM-NO3 - and environmental treatment,pathogen - and nitrogen,pathogen infection,nitrogen_botrytis-mycelium:quantity 2mM time 6hour . Plants were grown for 5 weeks on different nitrate concentrations. Rosette leaves were cut and infected (Inf) or not (NoInf) with Botrytis mycelium (B0510 strain) and placed in continuous light for 10 hours and harvested.


title: 10h-Inf_10mM-NO3_1

extract_protocol:
10h-Inf_10mM-NO3_1(100mg)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf

treatment_protocol: Name:10h-Inf_10mM-NO3 - and environmental treatment,pathogen - and nitrogen,pathogen infection,nitrogen_botrytis-mycelium:quantity 10mM time 6hour . Plants were grown for 5 weeks on different nitrate concentrations. Rosette leaves were cut and infected (Inf) or not (NoInf) with Botrytis mycelium (B0510 strain) and placed in continuous light for 10 hours and harvested.


title: 6h-Inf_0.5mM-NO3_1

extract_protocol:
6h-Inf_0.5mM-NO3_1(100mg)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: 10h-Inf_0.5mM-NO3_1

extract_protocol:
10h-Inf_0.5mM-NO3_1(100mg)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf

treatment_protocol: Name:10h-Inf_0.5mM-NO3 - and environmental treatment,pathogen - and nitrogen,pathogen infection,nitrogen_botrytis-mycelium:quantity 0.5mM time 10hour . Plants were grown for 5 weeks on different nitrate concentrations. Rosette leaves were cut and infected (Inf) or not (NoInf) with Botrytis mycelium (B0510 strain) and placed in continuous light for 10 hours and harvested.


title: 0h-NoInf_2mM-NO3_2

extract_protocol:
0h-NoInf_2mM-NO3_2(100mg)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: 0h-NoInf_10mM-NO3_3

extract_protocol:
0h-NoInf_10mM-NO3_3(100mg)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: 0h-NoInf_2mM-NO3_1

extract_protocol:
0h-NoInf_2mM-NO3_1(100mg)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: 0h-NoInf_10mM-NO3_2

extract_protocol:
0h-NoInf_10mM-NO3_2(100mg)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: 0h-NoInf_10mM-NO3_1

extract_protocol:
0h-NoInf_10mM-NO3_1(100mg)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: 0h-NoInf_2mM-NO3_3

extract_protocol:
0h-NoInf_2mM-NO3_3(100mg)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: 0h-NoInf_0.5mM-NO3_2

extract_protocol:
0h-NoInf_0.5mM-NO3_2(100mg)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: B73_DAP12_S_WW_2

extract_protocol:
B73_DAP12_S_WW_2(5ng)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: 0h-NoInf_0.5mM-NO3_1

extract_protocol:
0h-NoInf_0.5mM-NO3_1(100mg)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: mrMT microglia

status: Public on Jul 31 2020

city: Shanghai

first: Bo

last: Peng

address: 138 Yixueyuan Road

department: Institute for Translational Brain Research

country: China

institute: Fudan University

data_processing:
STAR
Cellranger
Genome_build: UCSC_mm10

source_name: Brain microglia

extract_protocol:
Cells were harvested by FACS, followed by the library preparation via 10x genomics protocol.
Followed the manufacture's protocol of 10x genomics.

value: Brain


title: 6h-Inf_2mM-NO3_2

extract_protocol:
6h-Inf_2mM-NO3_2(100mg)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: CS16 AoV CD34-enriched

status: Public on Sep 17 2020

first: Edie

last: Crosse

address: 5 Little France Road

department: Centre for Regenerative Medicine

institute: University of Edinburgh

data_processing:
Cell Ranger 2.1.0 (10x Genomics) analysis pipeline
Loompy: Conversion of expression data into loom file
SCANPY: Filtering cells with less than 200 genes, filtering out genes in less than 3 cells, filtering cells with a high percentage of mitochondrial genes (>0.05) and number of genes (>3500), normalising reads per cell.
Genome_build: Homo_sapiens.GRCh38
Supplementary_files_format_and_content: CSV, filtered raw count matrices with GENE ID as row names and Cell ID as column names

molecule: polyA RNA

source_name: Ventral dorsal aorta (AoV)

extract_protocol:
The dorsal aortae (Ao) were dissected from human embryos CS16 - sample 1 was further bisected into ventral (AoV) and dorsal (AoD) portions with only AoV taken for analysis, sample 2 was trimmed to the mid portion of the Ao. Tissues were dissociated into single cells in 1mg/ml Collagenase-Dispase. For sample 1 CD34+ cells were enriched using the human CD34 MicroBead Kit and LS MACS Columns (both Miltenyi Biotec). For sample 2, live cells were sorted using a FACS Aria-II (BD Bioscience) using 7AAD as a marker of dead cells.
Libraries were prepared using the Single Cell 3’ Reagent Kit v2 (10x Genomics) according to manufacturer's protocol.

tag: embryo

value: Ventral dorsal aorta (AoV)

tag: cell population


title: 6h-Inf_0.5mM-NO3_2

extract_protocol:
6h-Inf_0.5mM-NO3_2(100mg)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: ValAllier_ALL-036_3.

status: Public on Jun 03 2020

data_processing:
Genome_build: transcriptome of Ptrichocarpa primaryTranscriptOnly
Mapping and counts of reads by locus gene (tophat2/bowtie2). If PE reads, count only consistent PE reads
Supplementary_files_format_and_content: type:count file (txt); content:gene ID,length of Gene,nb single reads,nb PE reads

organism: Populus nigra

growth_protocol: Trees were pruned 2 times after the initial planting (in 2005). Planting space was organized in 4 blocks. The harvest date for RNA extraction was 4 years after the last pruning in winter 2011-2012.

extract_protocol:
ValAllier_ALL-036_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf

tag: population

value: ValAllier

tag: age

value: cambium,xylem


title: ValAllier_ALL-041_3.

extract_protocol:
ValAllier_ALL-041_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: ValAllier_ALL-036_1.

extract_protocol:
ValAllier_ALL-036_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: ValAllier_ALL-023_1.

extract_protocol:
ValAllier_ALL-023_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: ValAllier_ALL-041_1.

extract_protocol:
ValAllier_ALL-041_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: ValAllier_ALL-034_1.

extract_protocol:
ValAllier_ALL-034_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: ValAllier_ALL-023_3.

extract_protocol:
ValAllier_ALL-023_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: ValAllier_ALL-027_1.

extract_protocol:
ValAllier_ALL-027_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: ValAllier_ALL-026_3.

extract_protocol:
ValAllier_ALL-026_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: ValAllier_ALL-034_3.

extract_protocol:
ValAllier_ALL-034_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: ValAllier_ALL-026_1.

extract_protocol:
ValAllier_ALL-026_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: ValAllier_ALL-020_3.

extract_protocol:
ValAllier_ALL-020_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: 0h-NoInf_0.5mM-NO3_3

extract_protocol:
0h-NoInf_0.5mM-NO3_3(100mg)
rna_selection:selection polyA, paired-end, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: ValAllier_ALL-027_3.

extract_protocol:
ValAllier_ALL-027_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: ValAllier_ALL-021_3.

extract_protocol:
ValAllier_ALL-021_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: ValAllier_ALL-019_3.

extract_protocol:
ValAllier_ALL-019_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: ValAllier_ALL-020_1.

extract_protocol:
ValAllier_ALL-020_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: ValAllier_ALL-017_3.

extract_protocol:
ValAllier_ALL-017_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: ValAllier_ALL-013_3.

extract_protocol:
ValAllier_ALL-013_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: ValAllier_ALL-015_1.

extract_protocol:
ValAllier_ALL-015_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: ValAllier_ALL-017_1.

extract_protocol:
ValAllier_ALL-017_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: ValAllier_ALL-019_1.

extract_protocol:
ValAllier_ALL-019_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: ValAllier_ALL-014_3.

extract_protocol:
ValAllier_ALL-014_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: ValAllier_ALL-014_1.

extract_protocol:
ValAllier_ALL-014_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: ValAllier_ALL-012_3.

extract_protocol:
ValAllier_ALL-012_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: ValAllier_ALL-012_1.

extract_protocol:
ValAllier_ALL-012_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: ValAllier_ALL-011_3.

extract_protocol:
ValAllier_ALL-011_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: ValAllier_ALL-013_1.

extract_protocol:
ValAllier_ALL-013_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: ValAllier_ALL-011_1.

extract_protocol:
ValAllier_ALL-011_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: ValAllier_ALL-015_3.

extract_protocol:
ValAllier_ALL-015_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: ValAllier_ALL-008_3.

extract_protocol:
ValAllier_ALL-008_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_SN-7_1.

extract_protocol:
Ticino_SN-7_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf

value: Ticino


title: ValAllier_ALL-008_1.

extract_protocol:
ValAllier_ALL-008_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_SN-45_3.

extract_protocol:
Ticino_SN-45_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_SN-8_3.

extract_protocol:
Ticino_SN-8_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: ValAllier_ALL-006_3.

extract_protocol:
ValAllier_ALL-006_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: ValAllier_ALL-006_1.

extract_protocol:
ValAllier_ALL-006_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: ValAllier_ALL-005_3.

extract_protocol:
ValAllier_ALL-005_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: ValAllier_ALL-002_3.

extract_protocol:
ValAllier_ALL-002_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_SN-8_1.

extract_protocol:
Ticino_SN-8_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_SN-5_1.

extract_protocol:
Ticino_SN-5_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: ValAllier_ALL-005_1.

extract_protocol:
ValAllier_ALL-005_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_SN-4_3.

extract_protocol:
Ticino_SN-4_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_SN-4_1.

extract_protocol:
Ticino_SN-4_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_SN-45_1.

extract_protocol:
Ticino_SN-45_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_SN-2_1.

extract_protocol:
Ticino_SN-2_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_SN-2_3.

extract_protocol:
Ticino_SN-2_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_SN-42_1.

extract_protocol:
Ticino_SN-42_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_SN-5_3.

extract_protocol:
Ticino_SN-5_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_SN-42_3.

extract_protocol:
Ticino_SN-42_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_SN-3_1.

extract_protocol:
Ticino_SN-3_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_SN-43_3.

extract_protocol:
Ticino_SN-43_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_SN-38_3.

extract_protocol:
Ticino_SN-38_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_SN-38_1.

extract_protocol:
Ticino_SN-38_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_SN-33_1.

extract_protocol:
Ticino_SN-33_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_SN-33_3.

extract_protocol:
Ticino_SN-33_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_SN-7_3.

extract_protocol:
Ticino_SN-7_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_SN-25_3.

extract_protocol:
Ticino_SN-25_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_SN-29_1.

extract_protocol:
Ticino_SN-29_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_SN-29_3.

extract_protocol:
Ticino_SN-29_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_SN-26_1.

extract_protocol:
Ticino_SN-26_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_SN-43_1.

extract_protocol:
Ticino_SN-43_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_SN-24_3.

extract_protocol:
Ticino_SN-24_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_SN-24_1.

extract_protocol:
Ticino_SN-24_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_SN-23_3.

extract_protocol:
Ticino_SN-23_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_SN-22_1.

extract_protocol:
Ticino_SN-22_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_SN-3_3.

extract_protocol:
Ticino_SN-3_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_SN-19_1.

extract_protocol:
Ticino_SN-19_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_SN-1_1.

extract_protocol:
Ticino_SN-1_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_SN-26_3.

extract_protocol:
Ticino_SN-26_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_SN-25_1.

extract_protocol:
Ticino_SN-25_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_SN-18_1.

extract_protocol:
Ticino_SN-18_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_SN-19_3.

extract_protocol:
Ticino_SN-19_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_SN-23_1.

extract_protocol:
Ticino_SN-23_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_SN-17_3.

extract_protocol:
Ticino_SN-17_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_SN-16_1.

extract_protocol:
Ticino_SN-16_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_SN-16_3.

extract_protocol:
Ticino_SN-16_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_SN-12_3.

extract_protocol:
Ticino_SN-12_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_SN-22_3.

extract_protocol:
Ticino_SN-22_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_SN-12_1.

extract_protocol:
Ticino_SN-12_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-5_3.

extract_protocol:
Ticino_N-5_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-5_1.

extract_protocol:
Ticino_N-5_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-59_3.

extract_protocol:
Ticino_N-59_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-8_1.

extract_protocol:
Ticino_N-8_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-8_3.

extract_protocol:
Ticino_N-8_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-66_3.

extract_protocol:
Ticino_N-66_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-66_1.

extract_protocol:
Ticino_N-66_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-59_1.

extract_protocol:
Ticino_N-59_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-57_1.

extract_protocol:
Ticino_N-57_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-56_3.

extract_protocol:
Ticino_N-56_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-56_1.

extract_protocol:
Ticino_N-56_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-63_3.

extract_protocol:
Ticino_N-63_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_SN-20_3.

extract_protocol:
Ticino_SN-20_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_SN-14_1.

extract_protocol:
Ticino_SN-14_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-63_1.

extract_protocol:
Ticino_N-63_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-61_3.

extract_protocol:
Ticino_N-61_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-60_3.

extract_protocol:
Ticino_N-60_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-60_1.

extract_protocol:
Ticino_N-60_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_SN-17_1.

extract_protocol:
Ticino_SN-17_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-61_1.

extract_protocol:
Ticino_N-61_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-57_3.

extract_protocol:
Ticino_N-57_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-45_3.

extract_protocol:
Ticino_N-45_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-43_1.

extract_protocol:
Ticino_N-43_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_SN-14_3.

extract_protocol:
Ticino_SN-14_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-38_3.

extract_protocol:
Ticino_N-38_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-36_3.

extract_protocol:
Ticino_N-36_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-36_1.

extract_protocol:
Ticino_N-36_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-54_1.

extract_protocol:
Ticino_N-54_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-35_3.

extract_protocol:
Ticino_N-35_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-50_3.

extract_protocol:
Ticino_N-50_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-54_3.

extract_protocol:
Ticino_N-54_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-35_1.

extract_protocol:
Ticino_N-35_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-29_3.

extract_protocol:
Ticino_N-29_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-45_1.

extract_protocol:
Ticino_N-45_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-43_3.

extract_protocol:
Ticino_N-43_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-28_1.

extract_protocol:
Ticino_N-28_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-50_1.

extract_protocol:
Ticino_N-50_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-23_3.

extract_protocol:
Ticino_N-23_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-30_3.

extract_protocol:
Ticino_N-30_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-38_1.

extract_protocol:
Ticino_N-38_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-28_3.

extract_protocol:
Ticino_N-28_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-22_3.

extract_protocol:
Ticino_N-22_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-39_1.

extract_protocol:
Ticino_N-39_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-23_1.

extract_protocol:
Ticino_N-23_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-22_1.

extract_protocol:
Ticino_N-22_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-29_1.

extract_protocol:
Ticino_N-29_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-1_3.

extract_protocol:
Ticino_N-1_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-19_3.

extract_protocol:
Ticino_N-19_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-1_1.

extract_protocol:
Ticino_N-1_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-19_1.

extract_protocol:
Ticino_N-19_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-39_3.

extract_protocol:
Ticino_N-39_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-17_3.

extract_protocol:
Ticino_N-17_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-15_3.

extract_protocol:
Ticino_N-15_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-15_1.

extract_protocol:
Ticino_N-15_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-12_1.

extract_protocol:
Ticino_N-12_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-11_3.

extract_protocol:
Ticino_N-11_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_58-861_1.

extract_protocol:
Ticino_58-861_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Rhin_STR-022_3.

extract_protocol:
Rhin_STR-022_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf

value: Rhin


title: Ticino_58-861_3.

extract_protocol:
Ticino_58-861_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Rhin_STR-022_1.

extract_protocol:
Rhin_STR-022_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Rhin_STR-021_3.

extract_protocol:
Rhin_STR-021_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Rhin_STR-020_1.

extract_protocol:
Rhin_STR-020_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Rhin_STR-010_1.

extract_protocol:
Rhin_STR-010_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Rhin_STR-002_1.

extract_protocol:
Rhin_STR-002_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Rhin_RHN-039_3.

extract_protocol:
Rhin_RHN-039_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Rhin_STR-002_3.

extract_protocol:
Rhin_STR-002_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Rhin_STR-016_3.

extract_protocol:
Rhin_STR-016_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Rhin_RHN-041_3.

extract_protocol:
Rhin_RHN-041_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Rhin_STR-010_3.

extract_protocol:
Rhin_STR-010_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-17_1.

extract_protocol:
Ticino_N-17_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Rhin_STR-001_1.

extract_protocol:
Rhin_STR-001_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Rhin_STR-016_1.

extract_protocol:
Rhin_STR-016_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-12_3.

extract_protocol:
Ticino_N-12_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Rhin_RHN-041_1.

extract_protocol:
Rhin_RHN-041_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Rhin_RHN-039_1.

extract_protocol:
Rhin_RHN-039_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Rhin_RHN-038_3.

extract_protocol:
Rhin_RHN-038_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Rhin_RHN-038_1.

extract_protocol:
Rhin_RHN-038_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Rhin_RHN-031_1.

extract_protocol:
Rhin_RHN-031_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ticino_N-11_1.

extract_protocol:
Ticino_N-11_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Rhin_RHN-028_3.

extract_protocol:
Rhin_RHN-028_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Rhin_RHN-028_1.

extract_protocol:
Rhin_RHN-028_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ramieres_6-J13_3.

extract_protocol:
Ramieres_6-J13_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf

value: Ramieres


title: Ramieres_6-J17_1.

extract_protocol:
Ramieres_6-J17_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Rhin_ERS-018_3.

extract_protocol:
Rhin_ERS-018_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Rhin_ERS-017_3.

extract_protocol:
Rhin_ERS-017_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ramieres_6-J13_1.

extract_protocol:
Ramieres_6-J13_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Rhin_ERS-018_1.

extract_protocol:
Rhin_ERS-018_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ramieres_6-A26_3.

extract_protocol:
Ramieres_6-A26_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ramieres_6-J17_3.

extract_protocol:
Ramieres_6-J17_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Rhin_ERS-010_3.

extract_protocol:
Rhin_ERS-010_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ramieres_6-A18_1.

extract_protocol:
Ramieres_6-A18_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ramieres_6-A22_3.

extract_protocol:
Ramieres_6-A22_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Rhin_ERS-010_1.

extract_protocol:
Rhin_ERS-010_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Rhin_STR-001_3.

extract_protocol:
Rhin_STR-001_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ramieres_6-A16_1.

extract_protocol:
Ramieres_6-A16_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Rhin_RHN-031_3.

extract_protocol:
Rhin_RHN-031_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ramieres_6-A11_1.

extract_protocol:
Ramieres_6-A11_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ramieres_6-A11_3.

extract_protocol:
Ramieres_6-A11_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ramieres_6-A09_1.

extract_protocol:
Ramieres_6-A09_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ramieres_1-J31_1.

extract_protocol:
Ramieres_1-J31_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ramieres_6-J10_3.

extract_protocol:
Ramieres_6-J10_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ramieres_1-J19_3.

extract_protocol:
Ramieres_1-J19_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ramieres_6-J10_1.

extract_protocol:
Ramieres_6-J10_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ramieres_6-J02_1.

extract_protocol:
Ramieres_6-J02_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ramieres_6-A26_1.

extract_protocol:
Ramieres_6-A26_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ramieres_6-A30_3.

extract_protocol:
Ramieres_6-A30_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Rhin_ERS-017_1.

extract_protocol:
Rhin_ERS-017_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ramieres_6-A25_3.

extract_protocol:
Ramieres_6-A25_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ramieres_6-A30_1.

extract_protocol:
Ramieres_6-A30_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ramieres_6-A22_1.

extract_protocol:
Ramieres_6-A22_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ramieres_6-A25_1.

extract_protocol:
Ramieres_6-A25_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ramieres_6-A16_3.

extract_protocol:
Ramieres_6-A16_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ramieres_6-A09_3.

extract_protocol:
Ramieres_6-A09_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ramieres_1-J05_3.

extract_protocol:
Ramieres_1-J05_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ramieres_1-J07_1.

extract_protocol:
Ramieres_1-J07_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ramieres_1-J31_3.

extract_protocol:
Ramieres_1-J31_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ramieres_1-J16_3.

extract_protocol:
Ramieres_1-J16_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ramieres_1-J19_1.

extract_protocol:
Ramieres_1-J19_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ramieres_1-J16_1.

extract_protocol:
Ramieres_1-J16_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ramieres_1-J03_3.

extract_protocol:
Ramieres_1-J03_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ramieres_1-J03_1.

extract_protocol:
Ramieres_1-J03_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ramieres_1-J02_1.

extract_protocol:
Ramieres_1-J02_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ramieres_1-A26_3.

extract_protocol:
Ramieres_1-A26_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ramieres_1-A26_1.

extract_protocol:
Ramieres_1-A26_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ramieres_1-A25_3.

extract_protocol:
Ramieres_1-A25_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ramieres_1-J15_1.

extract_protocol:
Ramieres_1-J15_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ramieres_1-J15_3.

extract_protocol:
Ramieres_1-J15_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ramieres_1-A08_3.

extract_protocol:
Ramieres_1-A08_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ramieres_1-A02_3.

extract_protocol:
Ramieres_1-A02_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ramieres_1-A04_1.

extract_protocol:
Ramieres_1-A04_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ramieres_1-A04_3.

extract_protocol:
Ramieres_1-A04_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Paglia_PG-49_1.

extract_protocol:
Paglia_PG-49_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf

value: Paglia


title: Ramieres_1-J02_3.

extract_protocol:
Ramieres_1-J02_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Paglia_PG-43_3.

extract_protocol:
Paglia_PG-43_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Paglia_PG-43_1.

extract_protocol:
Paglia_PG-43_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Paglia_PG-49_3.

extract_protocol:
Paglia_PG-49_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ramieres_1-A09_1.

extract_protocol:
Ramieres_1-A09_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ramieres_1-A09_3.

extract_protocol:
Ramieres_1-A09_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ramieres_1-A02_1.

extract_protocol:
Ramieres_1-A02_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ramieres_1-J07_3.

extract_protocol:
Ramieres_1-J07_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ramieres_1-J05_1.

extract_protocol:
Ramieres_1-J05_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Paglia_PG-40_3.

extract_protocol:
Paglia_PG-40_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Paglia_PG-40_1.

extract_protocol:
Paglia_PG-40_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Paglia_PG-24_1.

extract_protocol:
Paglia_PG-24_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Paglia_PG-34_1.

extract_protocol:
Paglia_PG-34_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Paglia_PG-31_3.

extract_protocol:
Paglia_PG-31_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Paglia_PG-34_3.

extract_protocol:
Paglia_PG-34_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Ramieres_1-A25_1.

extract_protocol:
Ramieres_1-A25_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Paglia_PG-24_3.

extract_protocol:
Paglia_PG-24_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Paglia_PG-22_3.

extract_protocol:
Paglia_PG-22_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Paglia_PG-22_1.

extract_protocol:
Paglia_PG-22_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Paglia_PG-17_3.

extract_protocol:
Paglia_PG-17_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Paglia_PG-16_3.

extract_protocol:
Paglia_PG-16_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Paglia_PG-16_1.

extract_protocol:
Paglia_PG-16_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Paglia_PG-17_1.

extract_protocol:
Paglia_PG-17_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: NL_NL-2019_1.

extract_protocol:
NL_NL-2019_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf

value: NL


title: NL_NL-1421_3.

extract_protocol:
NL_NL-1421_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: NL_NL-1171_1.

extract_protocol:
NL_NL-1171_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: NL_NL-1322_1.

extract_protocol:
NL_NL-1322_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: NL_NL-2019_3.

extract_protocol:
NL_NL-2019_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Paglia_PG-15_1.

extract_protocol:
Paglia_PG-15_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: NL_NL-1322_3.

extract_protocol:
NL_NL-1322_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Paglia_PG-13_3.

extract_protocol:
Paglia_PG-13_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Paglia_PG-13_1.

extract_protocol:
Paglia_PG-13_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Loire_VDL-052_1.

extract_protocol:
Loire_VDL-052_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf

value: Loire


title: NL_NL-1421_1.

extract_protocol:
NL_NL-1421_1(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Paglia_PG-15_3.

extract_protocol:
Paglia_PG-15_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Paglia_PG-10_3.

extract_protocol:
Paglia_PG-10_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Paglia_PG-05_3.

extract_protocol:
Paglia_PG-05_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf


title: Loire_VDL-032_3.

extract_protocol:
Loire_VDL-032_3(5ug)
rna_selection:selection polyA, single, stranded:yes, Protocol:truseq-stranded-mrna-sample-prep-guide-15031047-E.pdf