title: Haemophilus influenzae RdKW20, WT, aerobic, rep1


title: SW480 cells, sgHSPB1, biol rep1, tech rep1

status: Public on Aug 20 2025

type: SRA

city: shanghai

first: Songguo

last: Zheng

address: No.621 Zhongshan 2nd Road, Songjiang District, Shanghai

country: China

institute: Shanghai Jiaotong University

description: Library name: sg1-2-2

library_source: transcriptomic

data_processing:
The sequencing data was filtered with SOAPnuke (v1.5.2)  by (1) Removing reads containing sequencing adapter; (2) Removing reads whose low-quality base ratio (base quality less than or equal to 5) is more than 20%; (3) Removing reads whose unknown base ('N' base) ratio is more than 5%, afterwards clean reads were obtained and stored in FASTQ format. The clean reads were mapped to the reference genome using HISAT2(v2.0.4) . Bowtie2 (v2.2.5) was applied to align the clean reads to the reference coding gene set， then expression level of gene was calculated by StringTie (v2.1.2)
Assembly: GRCh38
Supplementary files format and content: .txt include FPKM value for each sample

molecule: polyA RNA

organism: Homo sapiens

extract_protocol:
Total RNA was isolated from cell pellets, using the TRIzol.
Strand-specific libraries were generated using the VAHTS Universal V6 RNA-seq Library Prep Kit for MGI (NRM604-01) according to the manufacturer's instructions.

tag: cell line

tag: cell type

value: CRC cell


title: Biol rep 2 Acetyl-H3 24 h infection

city: Grand Forks

first: Barry

last: Milavetz

state: North Dakota

address: 1301 N Columbia Rd Stop 9037 RM W129

country: USA

institute: University of North Dakota School of Medicine and Health Sciences

library_source: genomic

data_processing:
Adapters were trimmed from fastq files with Cutadapt (V2.3)
Trimmed reads were aligned to the SV40 genome with STAR (V2.7.1a)
Aligned reads were filtered for properly paired reads and sorted with Samtools (1.10 using htslib V1.10)
Secondary reads were removed and the reads were sorted to position with Samtools (1.10 using htslib V1.10)
Duplicate reads were removed with Picard MarkDuplicates (V2.20.2 SNAPSHOT)
Fragments below 100bp or greater than 150bp were removed with Samtools (1.10 using htslib V1.10)
Bam files were indexed with Samtools (1.10 using htslib V1.10)
Bam files were normalized and bedgraphs were created with bamCoverage (V3.3.0)
Per basepair bedgraph files were created with R (V4.0.1 (2020-06-06) -- "See Things Now")
Replicate per basepair bedgraph files were merged with bedtools (V2.29.0) and averages of the replicates were calculated
heatmaps from the merged and averaged replicate samples were created in Python (V3.8)
Assembly: Reads were aligned to the SV40 genome (RefSeq Acc: NC_001669.1) cut at nucleotide (nt) 2666
Supplementary files format and content: bedgraph

molecule: genomic DNA

organism: Betapolyomavirus macacae

source_name: BSC-1 ATCC CCL-26

extract_protocol:
Chromatin immunoprecipitation (ChIP) analyses of the SV40 chromosomes obtained by treatment with DMSO, or inhibitor were performed according to the detailed description of the procedures previously described (Balakrishnan and Milavetz, 2017b;a). All the antibodies used were ChIP validated by their respective vendors. Antibodies (and their vendors) included: acetyl-H3 (06-599, Millipore), acetyl-H4 (06-866, Millipore), H3K9me1 (ab9045, Abcam), H3K27me3 (17-622 Millipore), and RNAPII (05-623-25G, Millipore). ChIPs were performed using Millipore kit Cat. No. 17-295 according to the supplier’s protocol. In a standard ChIP, 10 µl of antibody (10 µg) was incubated with the SV40 chromosomes being analyzed for 4 hours at 40C. A mixture of magnetic beads containing protein A and protein G was added (15 µl each) to each sample and incubated overnight. The next morning the magnetic beads and associated antibody bound chromatin was purified according to the protocol present in the kit.
The remaining DNA sample 25 µl was used for the preparation of a sequencing library. Libraries were prepared using the New England Biolabs Next UltraII kit according to the protocol supplied with the kit with one exception. All reagent volumes were reduced by half for the preparation of libraries with no apparent effect on library quality or quantity. Following its preparation, the library was purified AMPure magnetic beads in a two-step process. First, 7.5 µl of AMPure was added to the library (50 µl) in a 1.4 ml Eppindorf tube and incubated for 10 minutes to bind the DNA. The tube was incubated for 5 minutes in a magnetic rack and the liquid transferred to a new Eppendorf tube. An additional 20 µl of AMPure was added and incubated for 10 minutes. The tube was again placed on the magnetic rack and after a 5-minute incubation the liquid was removed and discarded. The magnetic beads were washed twice with 400 µl 70% ethanol, dried for 10 minutes, and resuspended in 20 µl nuclease-free water. In preliminary studies we found that this two-step purification yielded libraries enriched for nucleosome-sized insert DNA while also removing residual adapter from the library preparation. The size-selected library from each sample, 5 µl, was amplified by PCR using indices from New England Biolabs and purified on AMPure as previously described with minor modification (Balakrishnan and Milavetz, 2017a). Following PCR the amplification products, 170 µl were again purified in a two-step AMPure procedure. In the first step 60 µl of AMPure was added, the beads bound to a magnetic rack and the liquid transferred to a new tube. AMPure, 40 µl, was added to the liquid and the purification continued as previously described. This two-step process yielded a final sequencing library that was primarily in the size range of 150 to 300 base pairs in length including the adapter sequences from the library preparation.
ChIP samples for library preparation for ChIP-Seq was performed as above until the final elution step. Instead of eluting the intact SV40 chromatin bound to magnetic beads, the chromatin bound to beads was sonicated to fragment the chromatin and the fragments bound to the beads separated from the unbound fragments. The beads were washed twice with TE buffer from the Millipore kit. The DNA in the chromatin fragments still bound to the magnetic beads was removed by incubation for 15 minutes in the DNA binding buffer found in the NEB Monarch PCR and DNA Cleanup Kit. The DNA present in binding buffer was then purified according to the protocol found in the Monarch kit. In the final purification step with the kit, the DNA was eluted in 26 μl nuclease-free H2O. In order to estimate the amount of SV40 DNA present in the eluate, 1 µl was amplified along with a 1 µl sample of the SV40 chromatin used in the original ChIP using the early primers.

value: BSC-1 ATCC CCL-26

tag: treatment

value: Acetyl-H3 24 h infection


title: SW480 cells, sgHSPB1, biol rep1

description: Library name: sg1-2-1


title: LgA-LL-01

city: Baltimore

first: Atul

last: Daiwile

state: MD

address: 251 Bayview Blvd,

department: MNRB

institute: NIDA

data_processing:
Image analysis and base calling were conducted by the HiSeq Control Software (HCS). Raw sequence data (.bcl files) generated from Illumina HiSeq was converted into fastq files and de-multiplexed using Illumina's bcl2fastq 2.17 software. One mismatch was allowed for index sequence identification.
After investigating the quality of the raw data, sequence reads were trimmed to remove possible adapter sequences and nucleotides with poor quality using Trimmomatic v.0.36. The trimmed reads were mapped to the Rattus norvegicus reference genome available on ENSEMBL using the STAR aligner v.2.5.2b. The STAR aligner is a splice aligner that detects splice junctions and incorporates them to help align the entire read sequences. BAM files were generated as a result of this step. Unique gene hit counts were calculated by using feature Counts from the Subread package v.1.5.2. Only unique reads that fell within exon regions were counted.
After extraction of gene hit counts, the gene hit counts were added to the table
Assembly: Rattus norvegicus Rnor6.0
Supplementary files format and content: Txt file and counts

molecule: total RNA

organism: Rattus norvegicus

source_name: Dorsal striatum

extract_protocol:
RNA was extracted using the protocol from RNeasy Mini Handbook (Qiagen)
RNA samples were quantified using Qubit 2.0 Fluorometer (Life Technologies, Carlsbad, CA, USA) and RNA integrity was checked using Agilent TapeStation 4200 (Agilent Technologies, Palo Alto, CA, USA). RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly, mRNAs were first enriched with Oligo(dT) beads. Enriched mRNAs were fragmented for 15 minutes at 94 °C. First strand and second strand cDNAs were subsequently synthesized. cDNA fragments were end repaired and adenylated at 3’ends, and universal adapters were ligated to cDNA fragments, followed by index addition and library enrichment by limited-cycle PCR. The sequencing libraries were validated on the Agilent TapeStation (Agilent Technologies, Palo Alto, CA, USA), and quantified by using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA) as well as by quantitative PCR (KAPA Biosystems, Wilmington, MA, USA). The sequencing libraries were clustered on two flowcell lanes. After clustering, the flowcells were loaded on the Illumina HiSeq instrument (4000 or equivalent) according to manufacturer’s instructions. The samples were sequenced using a 2x150bp Paired End (PE) configuration.

treatment_protocol: Rats (n = 41) were randomly assigned to different groups: Saline (Sal) (n =8), Short-access (ShA) (n = 10), or Long-access (LgA) (n=23). On each training day, Long-access (LgA) and Sal rats were trained to self-administer for three sessions: one 3-h session during days 1–5, followed by two 3-h sessions during days 8–14, and then for three 3-h sessions during the rest of the study (days 15–20). Short-access (ShA) rats were trained to self-administer oxycodone for only one 3-h session for the entirety of the study (days 1–20). Rats were trained to self-administer oxycodone or saline for 20 days.

tag: tissue

value: Dorsal striatum

value: Neural tissue

tag: strain

value: Sprague Dawley

tag: genotype

value: wild type

value: Oxycodone SA Long-access low (LgA-L)


title: Goblet-Y/T

city: Boston

first: Xaralabos

last: Varelas

state: MA

address: 72 E. Concord St

department: Department of Biochemistry and Cell biology

institute: Boston University Chobanian & Avedisian School of Medicine

description: 10x Genomics

library_source: transcriptomic single cell

data_processing:
Samples were processed using the standard CellRanger pipeline (version 3.1.0)
Reads were mapped to mm10 supplemented with tdTomato reporter sequence
The data is processed using 10X Genomics’ Cell Ranger pipeline, which generates feature/barcode matrices from the raw counts data
Downstream analysis and additional QC and filtering were done on Seurat (version 5.0.1)
Assembly: mm10
Supplementary files format and content: h5 files

organism: Mus musculus

source_name: Lung

extract_protocol:
Lung tissues were harvested from mice and incubated with digestion media (RPMI, 2mg/ml collagenase A, and 0.1mg/ml DNaseI) at 37°C for 20 minutes on a rocker. The resulted single cell suspension was filtered through a 70um filter, then subsequently incubated with ACK lysis buffer for 3 minutes. Cells were stained with viability dye and were resuspend in FACS staining buffer (PBS, 2% FBS, 0.1% sodium azide). Cells were then stained with conjugated anti-CD45 antibody for 30 minutes at 4°C. Cells then were sorted to live CD45+ and CD45- populations. Then the two populations were mixed at 1 to 1 ratio for scRNA-seq.
Enzyme fragmentation, A tailing, adaptor ligation and PCR are performed to obtain final libraries containing P5 and P7 primers used in Illumina bridge amplification. Size distribution and molarity of resulting cDNA libraries were assessed via Bioanalyzer High Sensitivity DNA Assay (Agilent Technologies, USA).

treatment_protocol: Mice were administered tamoxifen (75 mg/kg) via intraperitoneal injection for five consecutive days. Lung tissues were harvested seven days after the final tamoxifen dose.

value: Lung

value: Tamoxifen


title: ShAR-05

value: Oxycodone SA Short-access (ShA)


title: Biol rep 4 Acetyl-H3 A366 treated

value: Acetyl-H3 A366 treated


title: SW480 cells, sgHSPB1, tech rep2

description: Library name: sg1-1-3


title: Scgb1a1CreERT- control

description: 10x Genomics


title: ShAR-04


title: Biol rep 2 Acetyl-H3 A366 treated


title: ShAR-03


title: mESC, H2AZ2 KI #3

city: Richmond

first: Hengbin

last: Wang

state: VA

address: 401 College Street

department: Massey Cancer Institute

institute: Virginia Commonwealth University

data_processing:
Adapters removed, low quality reads removed and low quality bases trimmed
Aligned to mm10 with STAR Aligner
Called peaks with SEACR
Assembly: mm10
Supplementary files format and content: bigwig files
Library strategy: Cut&Tag

source_name: mESC

extract_protocol:
The CUT&Tag assay was performed using the Hyperactive Universal CUT&Tag Assay Kit for Illumina (Vazyme Biotech, #TD903) following the manufacturers protocol.
For library amplification, 15 μl of purified DNA was mixed with 25 μl of 2× CAM, along with 5 μl of uniquely barcoded i5 and i7 primers from the TruePrep Index Kit V2 for Illumina (Vazyme Biotech, # TD202). A total volume of 50 μl of sample was placed in a thermal cycler using the following program: 72 °C for 3 min; 98 °C for 3 min; 12–16 cycles of 98 °C for 10 s, 60 °C for 5 s, and 72°C for 1 min; and holding at 12 °C. To purify the PCR products, 2× volumes of VAHTS DNA Clean Beads (Vazyme Biotech, #N411) were added and incubated at room temperature for 5 min. The beads were washed twice with 200 μl fresh 80% ethanol and eluted in 22 μl ddH2O.

value: mESC

value: H2AZ2 Knockin

tag: chip antibody

value: FLAG (diluted 1:100, Proteintech, Cat#20543-1-AP)


title: ShAR-02


title: SW480 cells, sgHSPB1

description: Library name: sg1-1-1


title: SW480 cells, sgNC, biol rep2, tech rep2

description: Library name: nc-3-3


title: mESC, H2AZ2 KI #4


title: Biol rep 3 Acetyl-H3 A366 treated


title: ShAR-01


title: Biol rep 4 Acetyl-H3 GSK-LSD1 treated

value: Acetyl-H3 GSK-LSD1 treated


title: Biol rep 1 Acetyl-H3 A366 treated


title: SW480 cells, sgNC, biol rep2, tech rep1

description: Library name: nc-3-2


title: mESC, H2AZ2 KI #2


title: mESC, H2AZ2 KI #1


title: SW480 cells, sgNC, biol rep2

description: Library name: nc-3-1


title: Biol rep 3 Acetyl-H3 GSK-LSD1 treated


title: Biol rep 2 Acetyl-H3 GSK-LSD1 treated


title: SW480 cells, sgNC, biol rep1, tech rep2

description: Library name: nc-2-3


title: Biol rep 1 Acetyl-H3 GSK-LSD1 treated


title: mESC, H2BC1 KI #2

value: H2BC1 Knockin

value: HA (diluted 1:100, Proteintech, Cat#51064-2-AP)


title: SW480 cells, sgNC, biol rep1, tech rep1

description: Library name: nc-2-2

value: CRC cell


title: SAL-R10

value: Saline Control


title: Biol rep 4 Acetyl-H3 DMSO treated

value: BSC-1 ATCC CCL-26

value: Acetyl-H3 DMSO treated


title: mESC, H2BC1 KI #3


title: SAL-R9

value: Dorsal striatum

value: Neural tissue

value: Sprague Dawley


title: SW480 cells, sgNC, biol rep1

description: Library name: nc-2-1


title: mESC, H2Aub #3

value: H2AK119ub (diluted 1:100, Cell Signaling Technology, 8240)


title: mESC, H2BC1 KI #1


title: Biol rep 3 Acetyl-H3 DMSO treated


title: SW480 cells, sgNC, tech rep2

description: Library name: nc-1-3


title: Biol rep 2 Acetyl-H3 DMSO treated


title: SAL-R7

value: wild type


title: SW480 cells, sgNC, tech rep1

description: Library name: nc-1-2


title: mESC, H2Aub #1


title: ShAL-10

value: Oxycodone SA Short-access (ShA)


title: mESC, H2Aub #2


title: Biol rep 1 Acetyl-H3 DMSO treated


title: SAL-12


title: 10dpi induced galls on tomato roots-Gall

status: Public on Aug 19 2025

city: Knoxville

first: Hewezi

last: Tarek

state: TN

address: 2621 Morgan Circle

institute: niversity of Tennessee, Knoxvile

description:
Library name: Toma-10dpi-Gall
10X genomics

data_processing:
The raw scRNA-seq data was analyzed using Cell Ranger 7.2.0 (https://www.10xgenomics.com/support/software/cell-ranger/downloads).
Assembly: Tomato (Solanum lycopersicum), SL3.0
Supplementary files format and content: Tab-separated values files and matrix files

molecule: nuclear RNA

organism:
Solanum lycopersicum
Meloidogyne incognita

source_name: Galls

growth_protocol: All experiments described in this study were conducted using the nematode susceptible tomato (Solanum lycopersicum) cultivar Heinz 1706. The plants were cultivated in controlled growth chambers maintained at 26°C with a 16-hour light and 8-hour dark photoperiod 65% humidity.

extract_protocol:
Frozen tissue samples from tomato root were transferred to SeqMatic LLC (Fremont, CA, USA) in dry ice for nuclei isolation, library preparation, and sequencing. Frozen tissue was carefully transferred to a gentleMACS M tube that was filled with 5 ml of Honda buffer (2.5% Ficoll 400, 5% Dextran T40, 0.4 M sucrose, 10 mM MgCl2, 1 µM DTT, 0.5% Triton X-100, 1 tablet/50 ml cOmplete Protease Inhibitor Cocktail (Roche), 0.4 U µl−1, Protector RNase Inhibitor (Sigma-Aldrich), 25 mM Tris-HCl, pH 7.4). This buffer composition enables efficient lysis of cell membranes while keeping the nuclear membranes intact. The M tubes were placed onto a gentleMACS Dissociator, where a specific program was executed at 4°C to disrupt the tissue and release the nuclei. The resulting suspension was filtered through a 70-µm strainer and centrifuged at 700 g for 10 min at 4°C. Finally, the pellet was resuspended carefully in 500 µl Honda buffer and filtered through a 30-µm strainer.
scRNA-seq libraries were generated using the Chromium Next GEM Single Cell 3ʹ Kit v3.1 following the manufacturer protocol (Manual part CG000315, Rev E; 10x Genomics). Library quality was validated using an Agilent TapeStation 4200 (High Sensitivity D1000 ScreenTape to evaluate library composition and size). Preliminary spike-in sequencing was performed and samples were pooled to equal read counts. Final library concentration was confirmed using qPCR on a Roche LightCycler 96.

treatment_protocol: Meloidogyne incognita infection

value: Galls

value: Infected


title: hrp1Δhrp3Δprf1Δ-expB-2

city: Vienna

first: Jian Yi

last: Kok

address: Dr.-Bohr-Gasse 3

country: Austria

institute: GMI - Gregor Mendel Institute of Molecular Plant Biology

description:
Library name: 313867
Experiment B

data_processing:
Data processing, including alignment and QC, was performed using the nf-core/mnaseseq pipeline (https://github.com/nf-core/mnaseseq), with further analysis using Deeptools (v3.3.1).
Assembly: ASM294v2
Supplementary files format and content: Provided are bigWig files representing the read-depth normalized and smoothed MNase-seq data for each sample.

organism: Schizosaccharomyces pombe

growth_protocol: Cells were revived from frozen (-70 ºC) stocks on YES plates at 32 ºC. Cells were then grown to saturation in YES at 32 ºC, subcultured and grown to mid-log (OD600 ~ 0.5) before being harvested.

extract_protocol:
MNase-seq was performed by adapting a published protocol (Lanterman et. al, 2009). Briefly, cells were fixed with formaldehyde, and converted to spheroplasts using Zymolyase. Chromatin was digested with MNase, and DNA was extracted using phenol-chloroform and ethanol precipitation. Mononucleosomal DNA was size-selected via agarose gel extraction.
Library preparation was performed using the NEBNext Ultra II kit (NEB Cat. No. E7645L) with custom barcoded adapters. Libraries were quality-checked using an Agilent Fragment Analyzer and quantified with a Kapa Library Quantification Kit (Roche Cat. No. KK4903). Sequencing was conducted on an Illumina sequencer.

treatment_protocol: No treatment was used.

value: h+ ade6-M216 ura4-D18 leu1-32 hrp1del::kanMX hrp3del prf1del


title: SW480 cells, sgNC

description: Library name: nc-1-1


title: prf1Δ-expB-2

description:
Library name: 313865
Experiment B

value: h+ ade6-M216 ura4-D18 leu1-32 prf1del


title: hrp1Δhrp3Δprf1Δ-expB-1

description:
Library name: 313866
Experiment B


title: SAL-R11


title: prf1Δ-expB-1

description:
Library name: 313864
Experiment B


title: SW480 cells, sgHSPB1, tech rep1

description: Library name: sg1-1-2


title: hrp1Δhrp3Δprf1Δ-expD-2

description:
Library name: 318086
Experiment D

data_processing:
Alignments, quality control, and initial processing of the raw fastq files or bam files from the sequencing run were performed using a custom pipeline.
Raw count files for each gene were subsequently generated using Kallisto.
Assembly: ASM294v2
Supplementary files format and content: Raw count tsv files for each sample are provided.

extract_protocol:
Total RNA was extracted from yeast cells using a standard hot acid phenol protocol, followed by phenol-chloroform-isopropanol (PCI) extraction and DNase digestion
RNA-seq libraries were prepared with polyA-tail enrichment using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina, followed by using the NEBNext Ultra II library preparation kit for Illumina complemented with custom synthesized barcoded sequencing adapters provided by the Vienna BioCenter Core Facilities Next Generation Sequencing Facility. PCR was performed as a 50 µl reaction using the Q5 polymerase (NEB). Samples were PCR-amplified to the beginning of the logarithmic phase, as determined using a small-scale qPCR reaction run in parallel (typically around 8-10 cycles). Following quality control by Agilent Fragment Analyzer and quantification by RT-qPCR using a Kapa Library Quantification Kit, libraries were sequenced at the Vienna BioCenter Core Facilities Next Generation Sequencing Core Facility on an Illumina sequencer.


title: prf1Δ-expD-3

description:
Library name: 318084
Experiment D


title: prf1Δ-expD-2

description:
Library name: 318083
Experiment D


title: hrp1Δhrp3Δ-expD-3

description:
Library name: 318081
Experiment D

value: h+ ade6-M216 ura4-D18 leu1-32 hrp1del::kanMX hrp3del


title: prf1Δ-expD-1

description:
Library name: 318082
Experiment D


title: hrp1Δhrp3Δprf1Δ-expD-3

description:
Library name: 318087
Experiment D


title: SAL-R8


title: wt-expD-3

description:
Library name: 318078
Experiment D


title: wt-expD-1

description:
Library name: 318076
Experiment D


title: hrp1Δhrp3Δ-expD-2

description:
Library name: 318080
Experiment D


title: hrp1Δhrp3Δ-expD-1

description:
Library name: 318079
Experiment D


title: wt-expD-2

description:
Library name: 318077
Experiment D


title: hrp1-CT(hrp3)-expC-2

description:
Library name: 313862
Experiment C

value: h+ ade6-M216 ura4-D18 leu1-32 hrp1del::hrp1-CT(hrp3) hrp3del


title: hrp3-CT(hrp1)-expC-2

description:
Library name: 313859
Experiment C

value: h+ ade6-M216 ura4-D18 leu1-32 hrp1del::kanMX hrp3del::hrp3-CT(hrp1)


title: hrp1-CT(hrp3)-expC-3

description:
Library name: 313863
Experiment C


title: 5dpi induced galls on tomato roots-Gall

description:
Library name: Toma-5dpi-Gall
10X genomics


title: wt-expC-3

description:
Library name: 313848
Experiment C


title: hrp3-CT(hrp1)-expC-3

description:
Library name: 313860
Experiment C


title: hrp3-CT(hrp1)-expC-1

description:
Library name: 313858
Experiment C


title: wt-expC-1

description:
Library name: 313846
Experiment C


title: wt-expC-2

description:
Library name: 313847
Experiment C


title: hrp1Δhrp3Δprf1Δ-expD-1

description:
Library name: 318085
Experiment D


title: hrp1-CT(hrp3)-expC-1

description:
Library name: 313861
Experiment C


title: JUNB ChIP-seq: HaCaT Replicate 3

city: Cincinnati

first: Matthew

last: Weirauch

state: Ohio

address: 3333 Burnet Avenue

department: Center for Autoimmune Genomics and Etiology (CAGE)

institute: Cincinnati Children's Hospital Medical Center

description: Library name: ChIP-seq_JUNB_HaCaT_RepC_E008084

data_processing:
We performed quality control of raw sequencing reads using the ChIP-seq transcription factor Pipeline v2.2.0 from the ENCODE Project (https://www.encodeproject.org/pipelines/).
ChIP-seq reads were aligned to the human genome (hg38) using Bowtie2. Aligned reads were then sorted using samtools (v.1.8) and duplicate reads were removed using Picard (v. 1.89) (https://broadinstitute.github.io/picard/).
Peaks were called using the default parameters of MACS2.
The ENCODE blacklist region was removed upon establishing the final peak set. The conservative overlap peaks were generated from the ENCODE pipeline.
Assembly: hg38
Supplementary files format and content: Aligned reads in bigWig format. Reads were normalized using deepTools bamCoverage with the parameters --normalizeUsing CPM --binSize 10
Supplementary files format and content: Peaks in narrowPeak format

source_name: HaCaT

growth_protocol: The HaCaT cell line was grown in DMEM medium supplemented with 10% FBS, 100 units/mL of penicillin, and 100 µg/mL of streptomycin.

extract_protocol:
Cells were crosslinked and nuclei were sonicated as described previously (Lu et al. 2015).
Libraries were prepared via ChIPmentation (Schmidl et al. 2015).

value: HaCaT

value: skin keratinocytes

tag: chip target

value: JUNB

value: Active Motif 39549 lot 34708001


title: JUNB ChIP-seq: HaCaT Replicate 1

description: Library name: ChIP-seq_JUNB_HaCaT_RepA_E008082


title: JUNB ChIP-seq: HaCaT Replicate 2

description: Library name: ChIP-seq_JUNB_HaCaT_RepB_E008083


title: FOSL2 ChIP-seq: HaCaT Replicate 3

description: Library name: ChIP-seq_FOSL2_HaCaT_RepC_E008087

value: CST 19967 lot 2


title: FOSL2 ChIP-seq: HaCaT Replicate 2

description: Library name: ChIP-seq_FOSL2_HaCaT_RepB_E008086


title: FOSL2 ChIP-seq: HaCaT Replicate 1

description: Library name: ChIP-seq_FOSL2_HaCaT_RepA_E008085


title: hPB_mRNA_WT_Rapa_d3

city: bethesda

first: Seolkyoung

last: Jung

address: 10 Center Dr

department: NIAMS

institute: NIH

description: Library name: hPB_mRNA_WT_Rapa_d3

data_processing:
base calling: illumina CASAVA 1.8.2
CRISPR screen: build index on artificial genome which have  specific sgRNA sequence and common template sequence with stagger sequence
CRISPR screen: bowtie/1.1.2 with -S -m 1 -p 4 -a --best --strata -n 2 -l 80
CRISPR screen: samtools idxstats and custom perl script to generate table for counts and fraction on each gene
ChIP-Seq_density: alignment: bowtie-1.1.1/bowtie -S -m 1 -p 20 -a --best --strata -n 2 -l 50
ChIP-Seq_density: filtering (uniquely aligned reads): samtools-0.1.19-1.2/samtools view -S -b -F4
ChIP-Seq_density: normalized tag density: custom script, maximum 2 reads with the same start position allowed
mRNA-Seq_density:alignment: gsnap --use-sarray=0 --novelsplicing=0 --use-splicing refseq.splices.iit --format=sam -B 4
mRNA-Seq_density: filtering: samtools-1.2/samtools view -S -b -F132 -q 20
mRNA-Seq density: normalized tag density: bedtools-2.25/genomeCoverageBed -ibam -bg -split -scale normalization_factor, ucsc-314/bedGraphToBigWig
Assembly: hg19
Supplementary files format and content: Tab-separated values files
Supplementary files format and content: bigwig file

source_name: blood

growth_protocol: SU-DHL-4 cells with doxycycline inducible Cas9 were described previously (Phelan et al. 2018). Cells were cultured in advanced RPMI-1640 media supplemented with 5% FBS (Gemini), 1% GlutaMAX, and 1% Penicillin-Streptomycin (Thermo Fisher Scientific). Peripheral blood mononuclear cells from healthy blood donors (NIH blood bank) were isolated over Ficoll and cryopreserved. Naïve CD19+ human B cells were isolated with EasySep Human Naïve B Cell Isolation Kit according to the manufacture’s instructions (Stemcell Technologies). Isolated B cells were grown in IMDM media supplemented with 10% FBS (Gemini), 50 µM of beta-mercaptoethanol, and 1% Penicillin-Streptomycin (Thermo Fisher Scientific). All cells were cultured at 37°C and 5% CO2 in a humidified incubator.

extract_protocol:
CRISPR screens:
SU-DHL-4 reporter cells were transduced with the Brunello library. For each screen, 6 replicates with 15 million cells (90 million in total) were transduced at a density of 5 million cells/ml in a 6 well plate. The amount of virus was adjusted according to the viral titer to obtain a multiplicity of infection (MOI) of 0.3. Cells were transduced by spinfection (1,460g for 90 min and 30°C) in the presence of 6 µg/ml polybrene. Infected cells were puromycin selected 24h post infection at a final concentration of 4 µg/ml. On day 3 post infection, the MOI was verified and cells were split in medium containing 200 ng/ml doxycycline. Cells were split every other day with fresh doxycycline containing medium. On day 10 post infection, for each of the six replicates 60 million cells were sorted using BD FACS Aria or Fusion cell sorters based on reporter fluorescence to capture the bottom and top 5% expressing cells. On average, for each screen 286 million cells were sorted yielding ~9 million low or high expressing cells (Table S1B). For the three mTORC1 inhibited CRISPR screens in BTG2, CD79B and OCAB reporter cell lines, 5 nM rapamycin was added on day 8 and cells were sorted on day 10 as for the other screens. Genomic DNA from sorted cells was extracted using DNeasy Blood and Tissue kit (Qiagen) according to the manufacturer’s instructions.
RNA-seq
Cells were either unperturbed, transduced with sgRNA, or treated with inhibitors as described above. Cells were split every other day and seeded at 200,000 cells/ml the day before harvest. Inhibitor treated cells were harvested on day 2 post-treatment and sgRNA transduced cells on day 10 post-infection. On the day of harvest, half a million cells were pelleted and lysed in 100 µl of the Ambion RNAqueous lysis solution. RNA was extracted and treated with DNase according to the RNAqueous-Micro Total RNA Isolation Kit protocol (Thermo Fisher Scientific). RNA-seq libraries were prepared from 500 ng of RNA using NEBNext Poly(A) mRNA Magnetic Isolation and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs).
ChIP-seq
Cells for ChIP-seq analysis were either unperturbed or transduced with sgRNA for 10 days before harvesting. The day before harvest, cells were diluted to 200,000 cells/ml. Cultured cells were fixed at room temperature for 10 min with 1% formaldehyde (Sigma) and the reaction was quenched with 125 mM glycine (Sigma). Fixed cells were washed with PBS, snap-frozen, and stored at -80°C until further processing. For ChIP, 20 million cells were resuspended in 1 ml of RIPA buffer (10 mM Tris pH 7.6, 1 mM EDTA, 0.1% SDS, 0.1% sodium deoxycholate, 1% Triton X-100, and freshly added Complete Mini EDTA free proteinase inhibitor (Roche)). Chromatin was sheared by sonication on ice using a Branson Sonifier with an amplitude of 35% and 12 cycles of 20 sec sonication followed by 30 sec of pause. Supernatant was precleared with 20 µl Dynabeads Protein A (Thermo Fisher Scientific) for 1 hour at 4°C under rotation. Precleared chromatin was incubated with BRD4 (Abcam, ab128874) or FLI1 (Abcam, ab133485) antibody overnight at 4°C under rotation. The antibody-protein solution was then captured with 40 µl of Dynabeads Protein A for 1 hour at 4°C under rotation. Antibody-bound beads were washed twice with RIPA buffer, twice with RIPA buffer containing 0.3 M NaCl, once with LiCl buffer (0.25M LiCl, 0.5% NP-40, 0.5% sodium deoxycholate), once with TE pH 8.0 containing 0.2% Triton X-100, and once with TE pH 8.0. Crosslinks were reversed by incubating the beads at 65°C for 4 hours in the presence of 0.3% SDS and 1 mg/ml Proteinase K (Thermo Fisher Scientific). ChIP DNA was column purified with ChIP DNA Clean and Concentrator kit (Zymo research).
RNA-seq
Libraries were prepared from 500 ng of RNA using NEBNext Poly(A) mRNA Magnetic Isolation and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). Single 50 cycles of sequencing data were acquired on HiSeq3000, NovaSeq6000, NovaSeqXplus (illumina).
ChIP-Seq
Libraries were prepared using the Ovation Ultralow Library System V2. Single 50 cycles of sequencing data were acquired on NextSeq550, NovaSeq6000 (illumina)
CRISPR screen
Libraries were prepared using staggered GeCKO readout primers following the recommended instructions (Shalem et al. 2014). Deep sequencing was performed on NextSeq550 and NovaSeq6000 (Illumina) using 75bp single-end reads.

treatment_protocol:
primary human B cells:
Cells were activated with 100 ng/ml MEGACD40L (Enzo Life Sciences), 1 µg/ml CpG oligodeoxynucleotide ODN 2006 (Invivogen), 50 ng/ml human IL-2, 50 ng/ml human IL-10, and 10 ng/ml human IL-15 (all interleukins from PeproTech). Every other day the medium was fully removed and cells diluted in fresh activation medium.
Inhibitor treatments:
SU-DHL-4 or primary B cells were seeded at 0.2 million cells/ml and treated with DMSO or inhibitors. Inhibitors were purchased from MedChemExpress or Selleck Chemicals and dissolved in DMSO. Inhibitors were titrated and used at the following concentrations: rapamycin (5 nM), MK2206 (250 nM), AS1842856 (50 nM), LY2090314 (2 nM), CHIR99021 (1 µM). After two days of treatment, cells were analyzed by flow cytometry or harvested for RNA-seq.

value: blood

value: primary B cell

value: WT

value: MEGACD40L, ODN, IL-2, IL-10, IL-15


title: hPB_mRNA_WT_DMSO_d1

description: Library name: hPB_mRNA_WT_DMSO_d1


title: hPB_mRNA_WT_DMSO_d3

description: Library name: hPB_mRNA_WT_DMSO_d3


title: hPB_mRNA_WT_DMSO_d2

description: Library name: hPB_mRNA_WT_DMSO_d2


title: SUDHL4_mRNA_WT_Rapa_r3

description: Library name: SUDHL4_mRNA_WT_Rapa_r3

value: B cell lymphoma

value: Rapamycin


title: hPB_mRNA_WT_Rapa_d2

description: Library name: hPB_mRNA_WT_Rapa_d2


title: hPB_mRNA_WT_Rapa_d1

description: Library name: hPB_mRNA_WT_Rapa_d1


title: SUDHL4_mRNA_WT_Rapa_r2

description: Library name: SUDHL4_mRNA_WT_Rapa_r2


title: SUDHL4_mRNA_WT_RapaGSK3i_r2

description: Library name: SUDHL4_mRNA_WT_RapaGSK3i_r2


title: SUDHL4_mRNA_WT_RapaGSK3i_r1

description: Library name: SUDHL4_mRNA_WT_RapaGSK3i_r1


title: SUDHL4_mRNA_WT_GSK3i_r3

description: Library name: SUDHL4_mRNA_WT_GSK3i_r3


title: SUDHL4_mRNA_WT_RapaGSK3i_r3

description: Library name: SUDHL4_mRNA_WT_RapaGSK3i_r3


title: SUDHL4_mRNA_WT_GSK3i_r2

description: Library name: SUDHL4_mRNA_WT_GSK3i_r2


title: SUDHL4_mRNA_WT_DMSO_r3

description: Library name: SUDHL4_mRNA_WT_DMSO_r3

value: DMSO


title: SUDHL4_mRNA_WT_GSK3i_r1

description: Library name: SUDHL4_mRNA_WT_GSK3i_r1


title: SUDHL4_mRNA_WT_DMSO_r1

description: Library name: SUDHL4_mRNA_WT_DMSO_r1


title: SUDHL4_mRNA_WT_DMSO_r2

description: Library name: SUDHL4_mRNA_WT_DMSO_r2


title: swr1Δ-expA-b1-1

description:
Library name: 177714
Experiment A, batch 1

value: h+ ade6-M216 ura4-D18 leu1-32 swr1del::kanMX


title: irc20Δ-expA-b1-1

description:
Library name: 177713
Experiment A, batch 1

value: h+ ade6-M216 ura4-D18 leu1-32 irc20del::kanMX


title: swr1Δ-expA-b1-3

description:
Library name: 177726
Experiment A, batch 1


title: irc20Δ-expA-b1-2

description:
Library name: 177719
Experiment A, batch 1


title: swr1Δ-expA-b1-2

description:
Library name: 177720
Experiment A, batch 1


title: rrp2Δ-expA-b1-3

description:
Library name: 177724
Experiment A, batch 1

value: h+ ade6-M216 ura4-D18 leu1-32 rrp2del::kanMX


title: rrp1Δ-expA-b1-2

description:
Library name: 177717
Experiment A, batch 1

value: h+ ade6-M216 ura4-D18 leu1-32 rrp1del::kanMX


title: rrp2Δ-expA-b1-1

description:
Library name: 177712
Experiment A, batch 1


title: irc20Δ-expA-b1-3

description:
Library name: 177725
Experiment A, batch 1


title: rrp1Δ-expA-b1-1

description:
Library name: 177711
Experiment A, batch 1


title: hrp3Δ-expA-b1-3

description:
Library name: 177705
Experiment A, batch 1

value: h+ ade6-M216 ura4-D18 leu1-32 hrp3del::kanMX


title: rrp2Δ-expA-b1-2

description:
Library name: 177718
Experiment A, batch 1


title: hrp1Δ-expA-b1-2

description:
Library name: 177696
Experiment A, batch 1

value: h+ ade6-M216 ura4-D18 leu1-32 hrp1del::kanMX


title: rrp1Δ-expA-b1-3

description:
Library name: 177723
Experiment A, batch 1


title: SUDHL4_mRNA_WT_Rapa_r1

description: Library name: SUDHL4_mRNA_WT_Rapa_r1


title: fft3Δ-expA-b1-2

description:
Library name: 177695
Experiment A, batch 1

value: h+ ade6-M216 ura4-D18 leu1-32 fft3del::kanMX


title: fft3Δ-expA-b1-3

description:
Library name: 177703
Experiment A, batch 1


title: mit1Δ-expA-b1-2

description:
Library name: 177698
Experiment A, batch 1

value: h+ ade6-M216 ura4-D18 leu1-32 mit1del::kanMX


title: mit1Δ-expA-b1-3

description:
Library name: 177706
Experiment A, batch 1


title: hrp1Δ-expA-b1-3

description:
Library name: 177704
Experiment A, batch 1


title: fft2Δ-expA-b1-3

description:
Library name: 177702
Experiment A, batch 1

value: h+ ade6-M216 ura4-D18 leu1-32 fft2del::kanMX


title: fft2Δ-expA-b1-2

description:
Library name: 177694
Experiment A, batch 1


title: mit1Δ-expA-b1-1

description:
Library name: 177690
Experiment A, batch 1


title: fft2Δ-expA-b1-1

description:
Library name: 177686
Experiment A, batch 1


title: fft3Δ-expA-b1-1

description:
Library name: 177687
Experiment A, batch 1


title: hrp3Δ-expA-b1-2

description:
Library name: 177697
Experiment A, batch 1


title: wt-expA-b1-3

description:
Library name: 177727
Experiment A, batch 1


title: CNTF_soma_STIM_2

city: Wuerzburg

first: Michael

last: Briese

state: Bavaria

address: Versbacherstr. 5

department: Institute of Clinical Neurobiology

country: Germany

institute: University Hospital Wuerzburg

description:
Library name: CNTF_soma_STIM_2
CNTF_soma_STIM_2

data_processing:
Base calling with Illumina NextSeq RTA v3.10.30 (NGF) and v4.12.2 (CNTF)
FASTQ conversion with bcl-convert (Illumina) v4.0.3 (NGF) and v4.2.4 (CNTF)
FASTQ quality and adapter trimming using Cutadapt (Martin, 2011) v2.5 (parameters: --nextseq-trim=20 -m 1 -a CTGTCTCTTATACACATCT)
Alignment of trimmed reads to mouse genome using STAR (Dobin et al., 2013) v2.7.2b with default parameters except for including transcript annotations from RefSeq annotation version 109 (NGF) and RS_2023_04 (CNTF) for GRCm39
Calculation of read counts on exon level summarized for each gene (RefSeq annotation version 109 [NGF] and RS_2023_04 [CNTF] for GRCm39) using featureCounts v1.6.4 from Subread package (Liao et al., 2014) (parameters: -s 0 -t exon -M -O --fraction)
Assembly: GCF_000001635.27/GRCm39
Supplementary files format and content: Character (TAB) separated value file containing raw read counts

source_name: dorsal root ganglia

growth_protocol: DRGs were extracted  from E13 mouse embryos and seeded into microfluidic chambers (Xona Microfluidics, IND150) pre-coated with 0.1% poly-L-lysine hydrobromide (Sigma-Alrich) and laminin-111 (Thermo Fisher Scientific) and maintained at 37°C, 5% CO2 in neurobasal medium (Gibco) supplemented with 2% B27 (Gibco), 2% heat-inactivated horse serum (Linaris), 500 µM GlutaMAX (Gibco).  The somatic compartment contained 10 ng/ml NGF, 5 ng/ml BDNF, 5 ng/ml GDNF and 5 ng/ml CNTF. The axonal compartment contained 40 ng/ml NGF, 5 ng/ml BDNF and 5 ng/ml CNTF.

extract_protocol:
RNA was purified from the somatodendritic and axonal compartment using the PicoPure RNA Isolation Kit (Thermo Fisher Scientific, KIT0204) with 10 μl elution volume.
cDNA libraries suitable for sequencing were prepared with SMART-Seq® v4 Ultra® Low Input RNA Kit (Takara) according to the manufacturer’s instructions (1/4 volume for the somatodendritic samples and full volume for the axon samples), followed by a tagmentation-based protocol (Nextera XT, Illumina) using a quarter of the recommended reagent volumes.

treatment_protocol: For NGF treatment, the concentration of NGF on the axonal side was lowered to 2 ng/ml on DIV 4 for 12 h, followed by 2 h of stimulation with 100 ng/ml NGF. For CNTF treatment, the axonal side was completely deprived of CNTF on DIV 4 for 24 h, followed by 2 h of stimulation with 100 ng/ml CNTF.

value: dorsal root ganglia

value: sensory neurons

value: CNTF

tag: compartment

value: soma


title: CNTF_axon_STIM_4

description:
Library name: CNTF_axon_STIM_4
CNTF_axon_STIM_4

value: axon


title: hrp3Δ-expA-b1-1

description:
Library name: 177689
Experiment A, batch 1


title: wt-expA-b1-2

description:
Library name: 177721
Experiment A, batch 1


title: CNTF_soma_CTRL_4

description:
Library name: CNTF_soma_CTRL_4
CNTF_soma_CTRL_4

value: CTRL


title: hrp1Δ-expA-b1-1

description:
Library name: 177688
Experiment A, batch 1


title: CNTF_axon_STIM_3

description:
Library name: CNTF_axon_STIM_3
CNTF_axon_STIM_3


title: CNTF_soma_STIM_4

description:
Library name: CNTF_soma_STIM_4
CNTF_soma_STIM_4


title: CNTF_axon_STIM_1

description:
Library name: CNTF_axon_STIM_1
CNTF_axon_STIM_1


title: wt-expA-b1-1

description:
Library name: 177715
Experiment A, batch 1


title: CNTF_soma_STIM_3

description:
Library name: CNTF_soma_STIM_3
CNTF_soma_STIM_3


title: CNTF_axon_STIM_2

description:
Library name: CNTF_axon_STIM_2
CNTF_axon_STIM_2


title: CNTF_soma_CTRL_1

description:
Library name: CNTF_soma_CTRL_1
CNTF_soma_CTRL_1


title: CNTF_axon_CTRL_3

description:
Library name: CNTF_axon_CTRL_3
CNTF_axon_CTRL_3


title: CNTF_axon_CTRL_4

description:
Library name: CNTF_axon_CTRL_4
CNTF_axon_CTRL_4


title: CNTF_soma_CTRL_2

description:
Library name: CNTF_soma_CTRL_2
CNTF_soma_CTRL_2


title: CNTF_axon_CTRL_2

description:
Library name: CNTF_axon_CTRL_2
CNTF_axon_CTRL_2


title: CNTF_axon_CTRL_1

description:
Library name: CNTF_axon_CTRL_1
CNTF_axon_CTRL_1


title: NGF_soma_STIM_3

description:
Library name: NGF_soma_STIM_3
NGF_soma_STIM_3

value: NGF


title: NGF_soma_STIM_1

description:
Library name: NGF_soma_STIM_1
NGF_soma_STIM_1


title: NGF_soma_STIM_2

description:
Library name: NGF_soma_STIM_2
NGF_soma_STIM_2


title: CNTF_soma_STIM_1

description:
Library name: CNTF_soma_STIM_1
CNTF_soma_STIM_1


title: NGF_axon_STIM_4

description:
Library name: NGF_axon_STIM_4
NGF_axon_STIM_4


title: NGF_axon_STIM_3

description:
Library name: NGF_axon_STIM_3
NGF_axon_STIM_3


title: NGF_axon_STIM_1

description:
Library name: NGF_axon_STIM_1
NGF_axon_STIM_1


title: NGF_axon_STIM_2

description:
Library name: NGF_axon_STIM_2
NGF_axon_STIM_2


title: NGF_soma_CTRL_4

description:
Library name: NGF_soma_CTRL_4
NGF_soma_CTRL_4


title: NGF_soma_CTRL_3

description:
Library name: NGF_soma_CTRL_3
NGF_soma_CTRL_3


title: NGF_soma_CTRL_2

description:
Library name: NGF_soma_CTRL_2
NGF_soma_CTRL_2


title: NGF_axon_CTRL_4

description:
Library name: NGF_axon_CTRL_4
NGF_axon_CTRL_4


title: NGF_soma_CTRL_1

description:
Library name: NGF_soma_CTRL_1
NGF_soma_CTRL_1


title: CNTF_soma_CTRL_3

description:
Library name: CNTF_soma_CTRL_3
CNTF_soma_CTRL_3


title: NGF_axon_CTRL_2

description:
Library name: NGF_axon_CTRL_2
NGF_axon_CTRL_2


title: NGF_soma_STIM_4

description:
Library name: NGF_soma_STIM_4
NGF_soma_STIM_4


title: NGF_axon_CTRL_3

description:
Library name: NGF_axon_CTRL_3
NGF_axon_CTRL_3


title: NGF_axon_CTRL_1

description:
Library name: NGF_axon_CTRL_1
NGF_axon_CTRL_1


title: experiment  RTase, condition: Superscript 50 °C, sample 4

city: Martinsried

first: Wolfgang

last: Enard

address: Grossshaderner Str.2, Anthropology Human Genomics, AG Enard

department: Anthropology and human genomics

institute: LMU Munich

description: Library name: RTase_S50_sample4

data_processing:
Quality assessment of the fastq data files was conducted using fastqc (v 0.11.5) [2], followed by polyA trimming using cutadapt (v 4.1) [3]. Subsequent read filtering, mapping, and counting were performed using the zUMIs pipeline (v 2.9.7) [4], with reads mapped to the mouse genome (mm10). Fastq files were sample-demultiplexed using deML [5].
2 Andrews, S. (2010). FastQC: A quality control tool for high throughput sequence data. Available at: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/
3 Martin, M. (2011). Cutadapt removes adapter sequences from high-throughput sequencing reads. EMBnet. journal, 17(1), 10-12.
4 Parekh, S., Ziegenhain, C., Vieth, B., Enard, W., & Hellmann, I. (2018). zUMIs- A fast and flexible pipeline to process RNA sequencing data with UMIs. GigaScience, 7(6), giy059.
5 Gabriel Renaud, Udo Stenzel, Tomislav Maricic, Victor Wiebe, Janet Kelso (2015) deML: robust demultiplexing of Illumina sequences using a likelihood-based approach, Bioinformatics, Volume 31, Issue 5, March 2015, Pages 770–772, https://doi.org/10.1093/bioinformatics/btu719
Assembly: GRCm39
Supplementary files format and content: processed data files contain the Barcode (BC) of each sample with the associated counts (N) per feature (type), e.g. Intergenic, Intron, Exon, Ambiguity and Unmapped.

source_name: brain

extract_protocol:
prime-seq - tissue lysis and homogenization in RLT+ buffer + 1 % B-Mercaptoethanol
prime-seq, the prime-seq original protocol  [1] , the original protocol with adaptions and the new, improved protocol  version (Pförtner et al, this study)
1 Janjic, A., Wange, L.E., Bagnoli, J.W. et al. Prime-seq, efficient and powerful bulk RNA sequencing. Genome Biol 23, 88 (2022). https://doi.org/10.1186/s13059-022-02660-8

value: brain


title: experiment  RTase, condition: Superscript 42 °C, sample 2

description: Library name: RTase_S42_sample2


title: experiment  RTase, condition: Superscript 42 °C, sample 1

description: Library name: RTase_S42_sample1


title: experiment  RTase, condition: Maxima 42 °C, sample 1

description: Library name: RTase_M42_sample1


title: experiment  RTase, condition: Maxima 50 °C, sample 1

description: Library name: RTase_M50_sample1


title: experiment  RTase, condition: Maxima 42 °C, sample 4

description: Library name: RTase_M42_sample4


title: experiment  RTase, condition: Superscript 42 °C, sample 4

description: Library name: RTase_S42_sample4


title: experiment PoP96, condition PTO, rep:3, sample 9

description: Library name: PoP96_3_PTO_sample9


title: experiment  RTase, condition: Superscript 50 °C, sample 3

description: Library name: RTase_S50_sample3


title: experiment  RTase, condition: Maxima 50 °C, sample 4

description: Library name: RTase_M50_sample4


title: experiment PoP96, condition PTO, rep:3, sample 8

description: Library name: PoP96_3_PTO_sample8


title: experiment  RTase, condition: Superscript 50 °C, sample 1

description: Library name: RTase_S50_sample1


title: experiment  RTase, condition: Maxima 50 °C, sample 2

description: Library name: RTase_M50_sample2


title: experiment  RTase, condition: Superscript 50 °C, sample 2

description: Library name: RTase_S50_sample2


title: experiment PoP96, condition PTO, rep:3, sample 5

description: Library name: PoP96_3_PTO_sample5


title: experiment PoP96, condition PTO, rep:3, sample 6

description: Library name: PoP96_3_PTO_sample6


title: experiment PoP96, condition PTO, rep:3, sample 4

description: Library name: PoP96_3_PTO_sample4


title: experiment PoP96, condition PTO, rep:3, sample 10

description: Library name: PoP96_3_PTO_sample10


title: experiment PoP96, condition PTO, rep:3, sample 3

description: Library name: PoP96_3_PTO_sample3


title: experiment  RTase, condition: Superscript 42 °C, sample 3

description: Library name: RTase_S42_sample3


title: experiment PoP96, condition PTO, rep:3, sample 2

description: Library name: PoP96_3_PTO_sample2


title: experiment PoP96, condition PTO, rep:3, sample 1

description: Library name: PoP96_3_PTO_sample1


title: experiment  RTase, condition: Maxima 50 °C, sample 3

description: Library name: RTase_M50_sample3


title: experiment PoP96, condition PTO, rep:2, sample 9

description: Library name: PoP96_2_PTO_sample9


title: experiment PoP96, condition PTO, rep:2, sample 8

description: Library name: PoP96_2_PTO_sample8


title: experiment PoP96, condition PTO, rep:2, sample 6

description: Library name: PoP96_2_PTO_sample6


title: experiment PoP96, condition PTO, rep:2, sample 7

description: Library name: PoP96_2_PTO_sample7


title: experiment  RTase, condition: Maxima 42 °C, sample 3

description: Library name: RTase_M42_sample3


title: experiment PoP96, condition PTO, rep:2, sample 5

description: Library name: PoP96_2_PTO_sample5


title: experiment  RTase, condition: Maxima 42 °C, sample 2

description: Library name: RTase_M42_sample2


title: experiment PoP96, condition PTO, rep:2, sample 1

description: Library name: PoP96_2_PTO_sample1


title: experiment PoP96, condition PTO, rep:1, sample 9

description: Library name: PoP96_1_PTO_sample9


title: experiment PoP96, condition PTO, rep:1, sample 8

description: Library name: PoP96_1_PTO_sample8


title: experiment PoP96, condition PTO, rep:1, sample 5

description: Library name: PoP96_1_PTO_sample5


title: experiment PoP96, condition PTO, rep:1, sample 7

description: Library name: PoP96_1_PTO_sample7


title: experiment PoP96, condition PTO, rep:2, sample 3

description: Library name: PoP96_2_PTO_sample3


title: experiment PoP96, condition PTO, rep:2, sample 10

description: Library name: PoP96_2_PTO_sample10


title: experiment PoP96, condition PTO, rep:1, sample 4

description: Library name: PoP96_1_PTO_sample4


title: experiment PoP96, condition PTO, rep:2, sample 4

description: Library name: PoP96_2_PTO_sample4


title: experiment PoP96, condition PTO, rep:3, sample 7

description: Library name: PoP96_3_PTO_sample7


title: experiment PoP96, condition PTO, rep:1, sample 3

description: Library name: PoP96_1_PTO_sample3


title: experiment PoP96, condition PTO, rep:1, sample 6

description: Library name: PoP96_1_PTO_sample6


title: experiment PoP96, condition PTO, rep:1, sample 2

description: Library name: PoP96_1_PTO_sample2


title: experiment PoP96, condition PTO, rep:2, sample 2

description: Library name: PoP96_2_PTO_sample2


title: experiment PoP96, condition old, rep:3, sample 6

description: Library name: PoP96_3_old_sample6


title: experiment PoP96, condition old, rep:3, sample 9

description: Library name: PoP96_3_old_sample9


title: experiment PoP96, condition old, rep:3, sample 4

description: Library name: PoP96_3_old_sample4


title: experiment PoP96, condition old, rep:3, sample 7

description: Library name: PoP96_3_old_sample7


title: experiment PoP96, condition old, rep:3, sample 3

description: Library name: PoP96_3_old_sample3


title: experiment PoP96, condition old, rep:3, sample 5

description: Library name: PoP96_3_old_sample5


title: experiment PoP96, condition PTO, rep:1, sample 10

description: Library name: PoP96_1_PTO_sample10


title: experiment PoP96, condition PTO, rep:1, sample 1

description: Library name: PoP96_1_PTO_sample1


title: experiment PoP96, condition old, rep:3, sample 11

description: Library name: PoP96_3_old_sample11


title: experiment PoP96, condition old, rep:3, sample 2

description: Library name: PoP96_3_old_sample2


title: experiment PoP96, condition old, rep:2, sample 9

description: Library name: PoP96_2_old_sample9


title: experiment PoP96, condition old, rep:3, sample 1

description: Library name: PoP96_3_old_sample1


title: experiment PoP96, condition old, rep:3, sample 10

description: Library name: PoP96_3_old_sample10


title: experiment PoP96, condition old, rep:2, sample 7

description: Library name: PoP96_2_old_sample7


title: experiment PoP96, condition old, rep:2, sample 3

description: Library name: PoP96_2_old_sample3


title: experiment PoP96, condition old, rep:2, sample 11

description: Library name: PoP96_2_old_sample11


title: experiment PoP96, condition old, rep:2, sample 8

description: Library name: PoP96_2_old_sample8


title: experiment PoP96, condition old, rep:2, sample 2

description: Library name: PoP96_2_old_sample2


title: experiment PoP96, condition old, rep:2, sample 6

description: Library name: PoP96_2_old_sample6


title: experiment PoP96, condition old, rep:3, sample 8

description: Library name: PoP96_3_old_sample8


title: experiment PoP96, condition old, rep:1, sample 9

description: Library name: PoP96_1_old_sample9


title: experiment PoP96, condition old, rep:1, sample 6

description: Library name: PoP96_1_old_sample6


title: experiment PoP96, condition old, rep:1, sample 8

description: Library name: PoP96_1_old_sample8


title: experiment PoP96, condition old, rep:1, sample 4

description: Library name: PoP96_1_old_sample4


title: experiment PoP96, condition old, rep:1, sample 7

description: Library name: PoP96_1_old_sample7


title: experiment PoP96, condition old, rep:1, sample 3

description: Library name: PoP96_1_old_sample3


title: experiment PoP96, condition old, rep:1, sample 11

description: Library name: PoP96_1_old_sample11


title: experiment PoP96, condition old, rep:1, sample 1

description: Library name: PoP96_1_old_sample1


title: experiment PoP96, condition old, rep:1, sample 10

description: Library name: PoP96_1_old_sample10


title: experiment PoP96, condition new, rep:3, sample 5

description: Library name: PoP96_3_new_sample5


title: experiment PoP96, condition new, rep:3, sample 8

description: Library name: PoP96_3_new_sample8


title: experiment PoP96, condition new, rep:3, sample 9

description: Library name: PoP96_3_new_sample9


title: experiment PoP96, condition new, rep:3, sample 6

description: Library name: PoP96_3_new_sample6


title: experiment PoP96, condition new, rep:3, sample 7

description: Library name: PoP96_3_new_sample7


title: experiment PoP96, condition new, rep:3, sample 4

description: Library name: PoP96_3_new_sample4


title: experiment PoP96, condition new, rep:3, sample 2

description: Library name: PoP96_3_new_sample2


title: experiment PoP96, condition old, rep:2, sample 4

description: Library name: PoP96_2_old_sample4


title: experiment PoP96, condition old, rep:2, sample 10

description: Library name: PoP96_2_old_sample10


title: experiment PoP96, condition old, rep:2, sample 5

description: Library name: PoP96_2_old_sample5


title: experiment PoP96, condition new, rep:3, sample 11

description: Library name: PoP96_3_new_sample11


title: experiment PoP96, condition new, rep:3, sample 3

description: Library name: PoP96_3_new_sample3


title: experiment PoP96, condition old, rep:2, sample 1

description: Library name: PoP96_2_old_sample1


title: experiment PoP96, condition old, rep:1, sample 5

description: Library name: PoP96_1_old_sample5


title: experiment PoP96, condition new, rep:3, sample 10

description: Library name: PoP96_3_new_sample10


title: experiment PoP96, condition new, rep:3, sample 1

description: Library name: PoP96_3_new_sample1


title: experiment PoP96, condition new, rep:2, sample 9

description: Library name: PoP96_2_new_sample9


title: experiment PoP96, condition new, rep:2, sample 6

description: Library name: PoP96_2_new_sample6


title: experiment PoP96, condition new, rep:2, sample 5

description: Library name: PoP96_2_new_sample5


title: experiment PoP96, condition old, rep:1, sample 2

description: Library name: PoP96_1_old_sample2


title: experiment PoP96, condition new, rep:2, sample 3

description: Library name: PoP96_2_new_sample3


title: experiment PoP96, condition new, rep:2, sample 4

description: Library name: PoP96_2_new_sample4


title: experiment PoP96, condition new, rep:2, sample 2

description: Library name: PoP96_2_new_sample2


title: experiment PoP96, condition new, rep:2, sample 10

description: Library name: PoP96_2_new_sample10


title: experiment PoP96, condition new, rep:1, sample 8

description: Library name: PoP96_1_new_sample8


title: experiment PoP96, condition new, rep:1, sample 9

description: Library name: PoP96_1_new_sample9


title: experiment PoP96, condition new, rep:2, sample 1

description: Library name: PoP96_2_new_sample1


title: experiment PoP96, condition new, rep:1, sample 4

description: Library name: PoP96_1_new_sample4


title: experiment PoP96, condition new, rep:1, sample 6

description: Library name: PoP96_1_new_sample6


title: experiment PoP96, condition new, rep:1, sample 7

description: Library name: PoP96_1_new_sample7


title: experiment PoP96, condition new, rep:1, sample 3

description: Library name: PoP96_1_new_sample3


title: experiment PoP96, condition new, rep:1, sample 5

description: Library name: PoP96_1_new_sample5


title: experiment PoP96, condition new, rep:1, sample 11

description: Library name: PoP96_1_new_sample11


title: experiment PoP96, condition new, rep:1, sample 2

description: Library name: PoP96_1_new_sample2


title: experiment PoP96, condition new, rep:2, sample 8

description: Library name: PoP96_2_new_sample8


title: experiment PoP64, condition PTO, rep:1, sample 6

description: Library name: PoP64_1_PTO_sample6


title: experiment PoP64, condition PTO, rep:1, sample 8

description: Library name: PoP64_1_PTO_sample8


title: experiment PoP96, condition new, rep:1, sample 10

description: Library name: PoP96_1_new_sample10


title: experiment PoP64, condition PTO, rep:1, sample 5

description: Library name: PoP64_1_PTO_sample5


title: experiment PoP96, condition new, rep:2, sample 7

description: Library name: PoP96_2_new_sample7


title: experiment PoP64, condition PTO, rep:1, sample 3

description: Library name: PoP64_1_PTO_sample3


title: experiment PoP64, condition PTO, rep:1, sample 7

description: Library name: PoP64_1_PTO_sample7


title: experiment PoP64, condition PTO, rep:1, sample 2

description: Library name: PoP64_1_PTO_sample2


title: experiment PoP96, condition new, rep:1, sample 1

description: Library name: PoP96_1_new_sample1


title: experiment PoP96, condition new, rep:2, sample 11

description: Library name: PoP96_2_new_sample11


title: experiment PoP64, condition old, rep:1, sample 8

description: Library name: PoP64_1_old_sample8


title: experiment PoP64, condition old, rep:1, sample 6

description: Library name: PoP64_1_old_sample6


title: experiment PoP64, condition old, rep:1, sample 5

description: Library name: PoP64_1_old_sample5


title: experiment PoP64, condition old, rep:1, sample 3

description: Library name: PoP64_1_old_sample3


title: experiment PoP64, condition PTO, rep:1, sample 1

description: Library name: PoP64_1_PTO_sample1


title: experiment PoP64, condition PTO, rep:1, sample 4

description: Library name: PoP64_1_PTO_sample4


title: experiment PoP64, condition old, rep:1, sample 2

description: Library name: PoP64_1_old_sample2


title: experiment poolsize, condition 920, sample 9

description: Library name: poolsize_920_sample9


title: experiment PoP64, condition old, rep:1, sample 1

description: Library name: PoP64_1_old_sample1


title: experiment poolsize, condition 920, sample 8

description: Library name: poolsize_920_sample8


title: experiment PoP64, condition old, rep:1, sample 7

description: Library name: PoP64_1_old_sample7


title: experiment poolsize, condition 920, sample 4

description: Library name: poolsize_920_sample4


title: experiment poolsize, condition 920, sample 7

description: Library name: poolsize_920_sample7


title: experiment poolsize, condition 920, sample 6

description: Library name: poolsize_920_sample6


title: experiment poolsize, condition 920, sample 3

description: Library name: poolsize_920_sample3


title: experiment poolsize, condition 920, sample 2

description: Library name: poolsize_920_sample2


title: experiment poolsize, condition 920, sample 14

description: Library name: poolsize_920_sample14


title: experiment poolsize, condition 920, sample 15

description: Library name: poolsize_920_sample15


title: experiment poolsize, condition 920, sample 16

description: Library name: poolsize_920_sample16


title: experiment poolsize, condition 920, sample 10

description: Library name: poolsize_920_sample10


title: experiment poolsize, condition 920, sample 13

description: Library name: poolsize_920_sample13


title: experiment poolsize, condition 920, sample 1

description: Library name: poolsize_920_sample1


title: experiment poolsize, condition 920, sample 11

description: Library name: poolsize_920_sample11


title: experiment poolsize, condition 920, sample 12

description: Library name: poolsize_920_sample12


title: experiment poolsize, condition 80, sample 7

description: Library name: poolsize_80_sample7


title: experiment poolsize, condition 80, sample 8

description: Library name: poolsize_80_sample8


title: experiment poolsize, condition 80, sample 6

description: Library name: poolsize_80_sample6


title: experiment poolsize, condition 80, sample 5

description: Library name: poolsize_80_sample5


title: experiment PoP64, condition old, rep:1, sample 4

description: Library name: PoP64_1_old_sample4


title: experiment poolsize, condition 80, sample 4

description: Library name: poolsize_80_sample4


title: experiment poolsize, condition 80, sample 1

description: Library name: poolsize_80_sample1


title: experiment poolsize, condition 80, sample 3

description: Library name: poolsize_80_sample3


title: experiment poolsize, condition 320, sample 7

description: Library name: poolsize_320_sample7


title: experiment poolsize, condition 320, sample 9

description: Library name: poolsize_320_sample9


title: experiment poolsize, condition 320, sample 8

description: Library name: poolsize_320_sample8


title: experiment poolsize, condition 80, sample 2

description: Library name: poolsize_80_sample2


title: experiment poolsize, condition 320, sample 4

description: Library name: poolsize_320_sample4


title: experiment poolsize, condition 320, sample 5

description: Library name: poolsize_320_sample5


title: experiment poolsize, condition 920, sample 5

description: Library name: poolsize_920_sample5


title: experiment poolsize, condition 320, sample 6

description: Library name: poolsize_320_sample6


title: experiment poolsize, condition 320, sample 3

description: Library name: poolsize_320_sample3


title: experiment poolsize, condition 320, sample 16

description: Library name: poolsize_320_sample16


title: experiment poolsize, condition 320, sample 2

description: Library name: poolsize_320_sample2


title: experiment poolsize, condition 320, sample 14

description: Library name: poolsize_320_sample14


title: experiment poolsize, condition 320, sample 13

description: Library name: poolsize_320_sample13


title: experiment poolsize, condition 320, sample 10

description: Library name: poolsize_320_sample10


title: experiment poolsize, condition 320, sample 15

description: Library name: poolsize_320_sample15


title: experiment poolsize, condition 320, sample 11

description: Library name: poolsize_320_sample11


title: experiment poolsize, condition 320, sample 1

description: Library name: poolsize_320_sample1


title: experiment poolsize, condition 320, sample 12

description: Library name: poolsize_320_sample12


title: experiment BBB, condition Std, sample 7

description: Library name: BBB_Std_sample7


title: experiment BBB, condition Std, sample 9

description: Library name: BBB_Std_sample9


title: experiment BBB, condition Std, sample 5

description: Library name: BBB_Std_sample5


title: experiment BBB, condition Std, sample 3

description: Library name: BBB_Std_sample3


title: experiment BBB, condition Std, sample 4

description: Library name: BBB_Std_sample4


title: experiment BBB, condition Std, sample 8

description: Library name: BBB_Std_sample8


title: experiment BBB, condition Std, sample 2

description: Library name: BBB_Std_sample2


title: experiment BBB, condition Std, sample 6

description: Library name: BBB_Std_sample6


title: experiment BBB, condition Std, sample 16

description: Library name: BBB_Std_sample16


title: experiment BBB, condition Std, sample 15

description: Library name: BBB_Std_sample15


title: experiment BBB, condition Std, sample 13

description: Library name: BBB_Std_sample13


title: experiment BBB, condition Std, sample 12

description: Library name: BBB_Std_sample12


title: experiment BBB, condition Std, sample 14

description: Library name: BBB_Std_sample14


title: experiment BBB, condition Std, sample 1

description: Library name: BBB_Std_sample1


title: experiment BBB, condition Std, sample 11

description: Library name: BBB_Std_sample11


title: experiment BBB, condition Resus, sample 8

description: Library name: BBB_Resus_sample8


title: experiment BBB, condition Resus, sample 9

description: Library name: BBB_Resus_sample9


title: experiment BBB, condition Resus, sample 7

description: Library name: BBB_Resus_sample7


title: experiment BBB, condition Resus, sample 6

description: Library name: BBB_Resus_sample6


title: experiment BBB, condition Std, sample 10

description: Library name: BBB_Std_sample10


title: experiment BBB, condition Resus, sample 5

description: Library name: BBB_Resus_sample5


title: experiment BBB, condition Resus, sample 2

description: Library name: BBB_Resus_sample2


title: experiment BBB, condition Resus, sample 4

description: Library name: BBB_Resus_sample4


title: experiment BBB, condition Resus, sample 14

description: Library name: BBB_Resus_sample14


title: experiment BBB, condition Resus, sample 13

description: Library name: BBB_Resus_sample13


title: experiment BBB, condition Resus, sample 15

description: Library name: BBB_Resus_sample15


title: experiment BBB, condition Resus, sample 16

description: Library name: BBB_Resus_sample16


title: experiment BBB, condition Resus, sample 12

description: Library name: BBB_Resus_sample12


title: experiment BBB, condition Resus, sample 11

description: Library name: BBB_Resus_sample11


title: experiment BBB, condition Resus, sample 10

description: Library name: BBB_Resus_sample10


title: experiment BBB, condition BBB, sample 9

description: Library name: BBB_BBB_sample9


title: experiment BBB, condition BBB, sample 6

description: Library name: BBB_BBB_sample6


title: experiment BBB, condition BBB, sample 7

description: Library name: BBB_BBB_sample7


title: experiment BBB, condition BBB, sample 3

description: Library name: BBB_BBB_sample3


title: experiment BBB, condition BBB, sample 4

description: Library name: BBB_BBB_sample4


title: experiment BBB, condition Resus, sample 1

description: Library name: BBB_Resus_sample1


title: experiment BBB, condition BBB, sample 8

description: Library name: BBB_BBB_sample8


title: experiment BBB, condition BBB, sample 5

description: Library name: BBB_BBB_sample5


title: experiment BBB, condition BBB, sample 14

description: Library name: BBB_BBB_sample14


title: experiment BBB, condition BBB, sample 2

description: Library name: BBB_BBB_sample2


title: experiment BBB, condition BBB, sample 13

description: Library name: BBB_BBB_sample13


title: experiment BBB, condition BBB, sample 15

description: Library name: BBB_BBB_sample15


title: experiment BBB, condition Resus, sample 3

description: Library name: BBB_Resus_sample3


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 3, sample 9

description: Library name: AdapPrim_3_nsn_sample9


title: experiment BBB, condition BBB, sample 10

description: Library name: BBB_BBB_sample10


title: experiment BBB, condition BBB, sample 16

description: Library name: BBB_BBB_sample16


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 3, sample 4

description: Library name: AdapPrim_3_nsn_sample4


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 3, sample 3

description: Library name: AdapPrim_3_nsn_sample3


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 3, sample 6

description: Library name: AdapPrim_3_nsn_sample6


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 3, sample 23

description: Library name: AdapPrim_3_nsn_sample23


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 3, sample 5

description: Library name: AdapPrim_3_nsn_sample5


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 3, sample 8

description: Library name: AdapPrim_3_nsn_sample8


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 3, sample 20

description: Library name: AdapPrim_3_nsn_sample20


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 3, sample 24

description: Library name: AdapPrim_3_nsn_sample24


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 3, sample 22

description: Library name: AdapPrim_3_nsn_sample22


title: experiment BBB, condition BBB, sample 1

description: Library name: BBB_BBB_sample1


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 3, sample 18

description: Library name: AdapPrim_3_nsn_sample18


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 3, sample 17

description: Library name: AdapPrim_3_nsn_sample17


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 3, sample 19

description: Library name: AdapPrim_3_nsn_sample19


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 3, sample 16

description: Library name: AdapPrim_3_nsn_sample16


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 3, sample 14

description: Library name: AdapPrim_3_nsn_sample14


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 3, sample 13

description: Library name: AdapPrim_3_nsn_sample13


title: experiment BBB, condition BBB, sample 12

description: Library name: BBB_BBB_sample12


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 3, sample 7

description: Library name: AdapPrim_3_nsn_sample7


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 3, sample 2

description: Library name: AdapPrim_3_nsn_sample2


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 3, sample 11

description: Library name: AdapPrim_3_nsn_sample11


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 2, sample 9

description: Library name: AdapPrim_2_nsn_sample9


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 3, sample 15

description: Library name: AdapPrim_3_nsn_sample15


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 2, sample 7

description: Library name: AdapPrim_2_nsn_sample7


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 2, sample 8

description: Library name: AdapPrim_2_nsn_sample8


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 3, sample 21

description: Library name: AdapPrim_3_nsn_sample21


title: experiment BBB, condition BBB, sample 11

description: Library name: BBB_BBB_sample11


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 3, sample 1

description: Library name: AdapPrim_3_nsn_sample1


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 2, sample 24

description: Library name: AdapPrim_2_nsn_sample24


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 2, sample 2

description: Library name: AdapPrim_2_nsn_sample2


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 3, sample 10

description: Library name: AdapPrim_3_nsn_sample10


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 2, sample 5

description: Library name: AdapPrim_2_nsn_sample5


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 2, sample 4

description: Library name: AdapPrim_2_nsn_sample4


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 2, sample 3

description: Library name: AdapPrim_2_nsn_sample3


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 2, sample 22

description: Library name: AdapPrim_2_nsn_sample22


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 2, sample 18

description: Library name: AdapPrim_2_nsn_sample18


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 2, sample 23

description: Library name: AdapPrim_2_nsn_sample23


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 2, sample 6

description: Library name: AdapPrim_2_nsn_sample6


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 2, sample 20

description: Library name: AdapPrim_2_nsn_sample20


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 2, sample 15

description: Library name: AdapPrim_2_nsn_sample15


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 2, sample 19

description: Library name: AdapPrim_2_nsn_sample19


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 2, sample 17

description: Library name: AdapPrim_2_nsn_sample17


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 2, sample 14

description: Library name: AdapPrim_2_nsn_sample14


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 2, sample 13

description: Library name: AdapPrim_2_nsn_sample13


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 2, sample 11

description: Library name: AdapPrim_2_nsn_sample11


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 2, sample 12

description: Library name: AdapPrim_2_nsn_sample12


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 1, sample 9

description: Library name: AdapPrim_1_nsn_sample9


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 2, sample 1

description: Library name: AdapPrim_2_nsn_sample1


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 1, sample 8

description: Library name: AdapPrim_1_nsn_sample8


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 1, sample 7

description: Library name: AdapPrim_1_nsn_sample7


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 2, sample 21

description: Library name: AdapPrim_2_nsn_sample21


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 1, sample 5

description: Library name: AdapPrim_1_nsn_sample5


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 1, sample 4

description: Library name: AdapPrim_1_nsn_sample4


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 1, sample 6

description: Library name: AdapPrim_1_nsn_sample6


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 1, sample 3

description: Library name: AdapPrim_1_nsn_sample3


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 1, sample 22

description: Library name: AdapPrim_1_nsn_sample22


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 2, sample 16

description: Library name: AdapPrim_2_nsn_sample16


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 1, sample 20

description: Library name: AdapPrim_1_nsn_sample20


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 1, sample 23

description: Library name: AdapPrim_1_nsn_sample23


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 1, sample 19

description: Library name: AdapPrim_1_nsn_sample19


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 1, sample 17

description: Library name: AdapPrim_1_nsn_sample17


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 1, sample 16

description: Library name: AdapPrim_1_nsn_sample16


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 1, sample 15

description: Library name: AdapPrim_1_nsn_sample15


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 1, sample 18

description: Library name: AdapPrim_1_nsn_sample18


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 1, sample 13

description: Library name: AdapPrim_1_nsn_sample13


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 1, sample 12

description: Library name: AdapPrim_1_nsn_sample12


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 1, sample 10

description: Library name: AdapPrim_1_nsn_sample10


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 2, sample 10

description: Library name: AdapPrim_2_nsn_sample10


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 1, sample 1

description: Library name: AdapPrim_1_nsn_sample1


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 1, sample 11

description: Library name: AdapPrim_1_nsn_sample11


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 3, sample 9

description: Library name: AdapPrim_3_nse_sample9


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 3, sample 7

description: Library name: AdapPrim_3_nse_sample7


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 3, sample 6

description: Library name: AdapPrim_3_nse_sample6


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 3, sample 12

description: Library name: AdapPrim_3_nsn_sample12


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 3, sample 4

description: Library name: AdapPrim_3_nse_sample4


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 3, sample 8

description: Library name: AdapPrim_3_nse_sample8


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 1, sample 24

description: Library name: AdapPrim_1_nsn_sample24


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 3, sample 23

description: Library name: AdapPrim_3_nse_sample23


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 1, sample 21

description: Library name: AdapPrim_1_nsn_sample21


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 3, sample 22

description: Library name: AdapPrim_3_nse_sample22


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 3, sample 24

description: Library name: AdapPrim_3_nse_sample24


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 3, sample 3

description: Library name: AdapPrim_3_nse_sample3


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 3, sample 20

description: Library name: AdapPrim_3_nse_sample20


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 3, sample 2

description: Library name: AdapPrim_3_nse_sample2


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 1, sample 2

description: Library name: AdapPrim_1_nsn_sample2


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 3, sample 21

description: Library name: AdapPrim_3_nse_sample21


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 3, sample 16

description: Library name: AdapPrim_3_nse_sample16


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 3, sample 18

description: Library name: AdapPrim_3_nse_sample18


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 3, sample 14

description: Library name: AdapPrim_3_nse_sample14


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 3, sample 13

description: Library name: AdapPrim_3_nse_sample13


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 3, sample 15

description: Library name: AdapPrim_3_nse_sample15


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 3, sample 12

description: Library name: AdapPrim_3_nse_sample12


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 3, sample 19

description: Library name: AdapPrim_3_nse_sample19


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 3, sample 10

description: Library name: AdapPrim_3_nse_sample10


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 3, sample 1

description: Library name: AdapPrim_3_nse_sample1


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 3, sample 11

description: Library name: AdapPrim_3_nse_sample11


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 2, sample 9

description: Library name: AdapPrim_2_nse_sample9


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 3, sample 5

description: Library name: AdapPrim_3_nse_sample5


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 2, sample 7

description: Library name: AdapPrim_2_nse_sample7


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 2, sample 8

description: Library name: AdapPrim_2_nse_sample8


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 2, sample 5

description: Library name: AdapPrim_2_nse_sample5


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 2, sample 4

description: Library name: AdapPrim_2_nse_sample4


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 2, sample 3

description: Library name: AdapPrim_2_nse_sample3


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 2, sample 24

description: Library name: AdapPrim_2_nse_sample24


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 2, sample 20

description: Library name: AdapPrim_2_nse_sample20


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 2, sample 6

description: Library name: AdapPrim_2_nse_sample6


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 2, sample 22

description: Library name: AdapPrim_2_nse_sample22


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 2, sample 2

description: Library name: AdapPrim_2_nse_sample2


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 2, sample 17

description: Library name: AdapPrim_2_nse_sample17


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 2, sample 19

description: Library name: AdapPrim_2_nse_sample19


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 2, sample 18

description: Library name: AdapPrim_2_nse_sample18


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 2, sample 16

description: Library name: AdapPrim_2_nse_sample16


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 3, sample 17

description: Library name: AdapPrim_3_nse_sample17


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 2, sample 15

description: Library name: AdapPrim_2_nse_sample15


title: experiment AdapPrim, condition original adapter , shortened P5 , original P7, rep 1, sample 14

description: Library name: AdapPrim_1_nsn_sample14


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 2, sample 12

description: Library name: AdapPrim_2_nse_sample12


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 2, sample 10

description: Library name: AdapPrim_2_nse_sample10


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 2, sample 14

description: Library name: AdapPrim_2_nse_sample14


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 2, sample 1

description: Library name: AdapPrim_2_nse_sample1


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 1, sample 9

description: Library name: AdapPrim_1_nse_sample9


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 1, sample 7

description: Library name: AdapPrim_1_nse_sample7


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 1, sample 5

description: Library name: AdapPrim_1_nse_sample5


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 1, sample 6

description: Library name: AdapPrim_1_nse_sample6


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 1, sample 4

description: Library name: AdapPrim_1_nse_sample4


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 1, sample 8

description: Library name: AdapPrim_1_nse_sample8


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 1, sample 3

description: Library name: AdapPrim_1_nse_sample3


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 1, sample 23

description: Library name: AdapPrim_1_nse_sample23


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 1, sample 21

description: Library name: AdapPrim_1_nse_sample21


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 1, sample 22

description: Library name: AdapPrim_1_nse_sample22


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 2, sample 23

description: Library name: AdapPrim_2_nse_sample23


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 2, sample 21

description: Library name: AdapPrim_2_nse_sample21


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 1, sample 2

description: Library name: AdapPrim_1_nse_sample2


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 1, sample 19

description: Library name: AdapPrim_1_nse_sample19


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 1, sample 20

description: Library name: AdapPrim_1_nse_sample20


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 1, sample 18

description: Library name: AdapPrim_1_nse_sample18


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 2, sample 13

description: Library name: AdapPrim_2_nse_sample13


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 1, sample 15

description: Library name: AdapPrim_1_nse_sample15


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 1, sample 17

description: Library name: AdapPrim_1_nse_sample17


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 2, sample 11

description: Library name: AdapPrim_2_nse_sample11


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 1, sample 16

description: Library name: AdapPrim_1_nse_sample16


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 1, sample 12

description: Library name: AdapPrim_1_nse_sample12


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 1, sample 13

description: Library name: AdapPrim_1_nse_sample13


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 1, sample 1

description: Library name: AdapPrim_1_nse_sample1


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 3, sample 9

description: Library name: AdapPrim_3_nnn_sample9


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 1, sample 11

description: Library name: AdapPrim_1_nse_sample11


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 1, sample 10

description: Library name: AdapPrim_1_nse_sample10


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 3, sample 8

description: Library name: AdapPrim_3_nnn_sample8


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 3, sample 7

description: Library name: AdapPrim_3_nnn_sample7


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 1, sample 24

description: Library name: AdapPrim_1_nse_sample24


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 3, sample 5

description: Library name: AdapPrim_3_nnn_sample5


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 3, sample 4

description: Library name: AdapPrim_3_nnn_sample4


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 3, sample 6

description: Library name: AdapPrim_3_nnn_sample6


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 3, sample 23

description: Library name: AdapPrim_3_nnn_sample23


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 3, sample 22

description: Library name: AdapPrim_3_nnn_sample22


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 3, sample 24

description: Library name: AdapPrim_3_nnn_sample24


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 3, sample 2

description: Library name: AdapPrim_3_nnn_sample2


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 3, sample 20

description: Library name: AdapPrim_3_nnn_sample20


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 3, sample 17

description: Library name: AdapPrim_3_nnn_sample17


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 3, sample 21

description: Library name: AdapPrim_3_nnn_sample21


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 3, sample 19

description: Library name: AdapPrim_3_nnn_sample19


title: experiment AdapPrim, condition original adapter , shortened P5 , extended P7, rep 1, sample 14

description: Library name: AdapPrim_1_nse_sample14


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 3, sample 18

description: Library name: AdapPrim_3_nnn_sample18


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 3, sample 16

description: Library name: AdapPrim_3_nnn_sample16


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 3, sample 14

description: Library name: AdapPrim_3_nnn_sample14


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 3, sample 15

description: Library name: AdapPrim_3_nnn_sample15


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 3, sample 11

description: Library name: AdapPrim_3_nnn_sample11


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 3, sample 12

description: Library name: AdapPrim_3_nnn_sample12


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 3, sample 10

description: Library name: AdapPrim_3_nnn_sample10


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 3, sample 1

description: Library name: AdapPrim_3_nnn_sample1


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 2, sample 9

description: Library name: AdapPrim_2_nnn_sample9


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 3, sample 13

description: Library name: AdapPrim_3_nnn_sample13


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 2, sample 8

description: Library name: AdapPrim_2_nnn_sample8


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 2, sample 5

description: Library name: AdapPrim_2_nnn_sample5


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 2, sample 6

description: Library name: AdapPrim_2_nnn_sample6


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 2, sample 7

description: Library name: AdapPrim_2_nnn_sample7


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 2, sample 4

description: Library name: AdapPrim_2_nnn_sample4


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 2, sample 3

description: Library name: AdapPrim_2_nnn_sample3


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 3, sample 3

description: Library name: AdapPrim_3_nnn_sample3


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 2, sample 21

description: Library name: AdapPrim_2_nnn_sample21


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 2, sample 24

description: Library name: AdapPrim_2_nnn_sample24


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 2, sample 20

description: Library name: AdapPrim_2_nnn_sample20


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 2, sample 23

description: Library name: AdapPrim_2_nnn_sample23


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 2, sample 19

description: Library name: AdapPrim_2_nnn_sample19


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 2, sample 17

description: Library name: AdapPrim_2_nnn_sample17


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 2, sample 15

description: Library name: AdapPrim_2_nnn_sample15


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 2, sample 18

description: Library name: AdapPrim_2_nnn_sample18


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 2, sample 16

description: Library name: AdapPrim_2_nnn_sample16


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 2, sample 14

description: Library name: AdapPrim_2_nnn_sample14


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 2, sample 13

description: Library name: AdapPrim_2_nnn_sample13


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 2, sample 10

description: Library name: AdapPrim_2_nnn_sample10


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 2, sample 11

description: Library name: AdapPrim_2_nnn_sample11


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 1, sample 8

description: Library name: AdapPrim_1_nnn_sample8


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 2, sample 1

description: Library name: AdapPrim_2_nnn_sample1


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 1, sample 7

description: Library name: AdapPrim_1_nnn_sample7


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 1, sample 9

description: Library name: AdapPrim_1_nnn_sample9


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 1, sample 5

description: Library name: AdapPrim_1_nnn_sample5


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 1, sample 3

description: Library name: AdapPrim_1_nnn_sample3


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 1, sample 24

description: Library name: AdapPrim_1_nnn_sample24


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 2, sample 22

description: Library name: AdapPrim_2_nnn_sample22


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 1, sample 23

description: Library name: AdapPrim_1_nnn_sample23


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 1, sample 20

description: Library name: AdapPrim_1_nnn_sample20


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 1, sample 2

description: Library name: AdapPrim_1_nnn_sample2


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 2, sample 2

description: Library name: AdapPrim_2_nnn_sample2


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 1, sample 18

description: Library name: AdapPrim_1_nnn_sample18


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 1, sample 17

description: Library name: AdapPrim_1_nnn_sample17


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 1, sample 16

description: Library name: AdapPrim_1_nnn_sample16


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 1, sample 15

description: Library name: AdapPrim_1_nnn_sample15


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 1, sample 10

description: Library name: AdapPrim_1_nnn_sample10


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 1, sample 13

description: Library name: AdapPrim_1_nnn_sample13


title: experiment AdapPrim, condition original adapter , original P5 , original P7, rep 2, sample 12

description: Library name: AdapPrim_2_nnn_sample12