title: Whole blood, KY-0034-11, PETCT1, Subclinical TB, Hot LN_Yes, Hot Lung_Yes


title: ExT6_day14_Tumor_Ly108_3

status: Public on Aug 09 2025

type: SRA

city: Noda City Chiba

first: Mikiya

last: Tsunoda

address: 2669 Yamazaki

department: Research Institute for Biomedical Sciences

country: Japan

institute: Tokyo university of science

description: Library name: Sample 8

library_source: transcriptomic

data_processing:
For bulk TCR analysis, adapter trimming and quality filtering of sequencing data were performed by using Cutadapt-3.2 and PRINSEQ-0.20.4. Sequencing data were processed by MiXCR-3.0.5. In MiXCR, Filtered reads were aligned to reference mouse TCR V/D/J sequences registered in the international ImMunoGeneTics information system: -starting-material=rna, -5-end=no-v-primers-, -3-end=c-primers, -adapters=no-adapters, vParameters.geneFeatureToAlign=VTranscript, -vjAlignmentOrder=JThenV, then identical sequences were assembled and grouped in clones with PCR and sequencing error correlation with the following parameters: -badQualityThreshold=15, –separateByV=true, –separateByJ=true, -only-productive=true, –region-of-interest=CDR3. The Variable (V) and Joining (J) segment of TCRs were represented in IMGT gene nomenclature. List of final clones were analyzed by VDJtools-1.2.1. Then, the sequencing reads of sample was normalized to the six times of cell count in each sample by “DownSample” command of VDJtools. T-cell clones were determined as TCR reads with the same TCR V segment, J segment and CDR3 nucleotide sequence.
For single cell analysis, reads were trimmed with Cutadapt-2.1051. Filtered reads were then annotated using the Python script provided by BD Biosciences and mapped to reference RNA using Bowtie2-2.4.254. Cell barcode information of each read was then added to the bowtie2-mapped BAM files and read counts of each gene in each cell barcode were counted using mawk. Resultant count data were converted to a single-cell gene-expression matrix file and filtered for valid cells based on read counts. Expression data of targeted panel genes were converted to a Seurat object55, log normalized and scaled (regressed by the number of unique molecular identifiers per cell) using the NormalizeData and ScaleData functions respectively. Principal component analysis (PCA) was performed using RunPCA function, and enrichment of each PC was calculated using the JackStraw and ScoreJackStraw function, and PCs that were significantly enriched (P ≤ 0.05) were selected for clustering and dimensional reduction analysis. Dimensional reduction was performed using RunUMAP function with default settings. Cell clustering was performed using FindNeighbors and FindClusters (resolution = 0.6) against the significant PCs.
Assembly: IMGT reference sequences of mouse TRB, GRCh38
Supplementary files format and content: text files in VDJtools format include read count and frequency, complementarity determining region 3 (CDR3) nucleotide and amino acid sequences, Variable(V)/ Diversity(D)/ Joining(J) segment names, and V, D and J segment boundaries within CDR3 nucleotide sequence of individual clones
Supplementary files format and content: tab-delimited text files include raw tag-count values for each sample
Supplementary files format and content: h5ad for scRNA-seq samples

molecule: polyA RNA

organism: Mus musculus

source_name: Tumor

growth_protocol: Lewis lung carcinoma (LLC) cells (5 × 10^5 cells /mouse) were inoculated subcutaneously (s.c.) into the left and right flanks of C57BL/6 mice.

extract_protocol:
Tumors were cut into small fragments and digested for 45 minutes at 37°C with 0.2% collagenase (FUJIFILM Wako) and DNase I (Sigma-Aldrich), except for experiments for single-cell analysis, in which tumors were digested with 0.2% collagenase NB8 (Nordmark Pharma GmbH). The cells were then subjected to two-phase density separation with upper 35% Percoll PLUS (GE Healthcare) (1000g, 10 minutes at room temperature). Leukocytes were recovered, washed and resuspended in preparation medium (PM) [5%FBS, 10mM HEPES (Nacalai), Penicillin-Streptomycin (Nacalai) in RPMI-1640 (Nacalai)]. After RBC lysis with ACK buffer [155mM of NH4Cl (Nacalai), 10mM of KHCO3 (Nacalai), and 0.5mM EDTA pH8.0 (NIPPON GENE)] for 1 minute at room temperature, cells were washed and resuspended in PM, and the cell concentrations were measured using Flow-Count fluorospheres (Beckman Coulter) and a CytoFLEX S flow cytometer (Beckman Coulter).Cells were stained with a mix of Fc Block (anti-mouse CD16/CD32 mAb; clone 2.4G2, BioLegend) and fluorophore-conjugated anti-mouse monoclonal antibodies (Supplementary Table 5). Antibody mixtures were prepared using BD Horizon Brilliant Stain Buffer Plus (BD) diluted 5x with FACS buffer (D-PBS(-) supplemented with 2% FBS, and 0.05% Sodium Azide). Staining was performed for 20 minutes at 4°C. In the single-cell RNA-seq/TCR-seq experiment, cells were stained with antibodies and anti-CD45 Sample Tag oligonucleotide-conjugated antibodies from the Single-Cell Multiplexing Kit (BD Biosciences). Before sorting, T cells were enriched from tumor suspensions by further staining with BD IMag APC Magnetic Particles-DM (BD Biosciences), then placed on magnetic plate. After washing by MACS buffer (D-PBS(-) supplemented with 10% BSA, and 2 mM EDTA pH8.0), APC and APC-Cy7(CD8, mouse TCRb)-positive cells were collected as positive fraction. Cell sorting was performed using a FACS Aria II or Aria III (BD Biosciences). Cells were stained with 5µg/mL of propidium iodide (PI, Sigma-Aldrich) immediately before sorting. Nonviable cells were excluded from the analysis based on forward and side scatter profiles and PI staining. For bulk TCR repertoire analysis and bulk RNA-seq analysis, cells were sorted into Lysis buffer [1% w/v LiDS, 100 mM Tris-HCl pH7.5, 500 mM LiCl, 5 mM dithiothreitol, and 10 mM EDTA pH8.0] and stored at −80°C. For single-cell RNA/TCR-seq, cells were sorted into D-PBS(-) with 10% FBS. For the bulk TCR-seq experiments, cells were sorted from the CD8+, CD8+PD-1+Ly108+TIM-3-, CD8+PD-1+Ly108+TIM-3+, CD8+PD-1+Ly108-TIM-3+ and CD8+PD-1- fractions to produce five datasets per mouse. For the single-cell RNA/TCR-seq and bulk TCR-seq experiment, cells were sorted from the CD8+PD-1+Ly108+TIM-3-, CD8+PD-1+Ly108+TIM-3+, CD8+PD-1+Ly108-TIM-3+ and CD8+PD-1- fractions.
Bulk TCR libraries were prepared on purified T cells lysed in lysis buffer [1% LiDS, 100 mM Tris-HCl (pH 7.5), 500 mM LiCl, and 10 mM EDTA]. PolyA RNAs were isolated according to a previous report with some modifications (GSE110711).To perform reverse transcription and template-swiching, beads were then suspended in 10 µL of RT mix [1× First Strand buffer (Thermo Fisher Scientific), 1 mM dNTP, 2.5 mM DTT (Thermo Fisher Scientific), 1 M betaine (Sigma-Aldrich), 9 mM MgCl2, 1 U/µL RNaseIn Plus Rnase Inhibitor (Promega), 10 U/µL Superscript II (Thermo Fisher Scientific), and 1µM of i5-TSO], and incubated for 60 min at 42ºC and immediately cooled on ice. Beads were washed once with with B&W-T buffer [5 mM Tris-HCl (pH 7.5), 1 M NaCl, 0.5 mM EDTA, and 0.1% Tween-20], and once with Tris-HCl (pH 8.0). To amplify the TCR cDNA containing complementarity determining region 3 (CDR3), nested PCR of the TCR locus was performed as follows. Beads with containing cDNA was resuspended with the 25 µL of first PCR mixture [0.4 µM of primers (i5, Trac_ex, and Trbc_ex), and 1x KAPA Hifi Hotstart ReadyMix (KAPA Biosystems, Wilmington, MA, #KK2602)], and the thermal cycling was performed as following condition: denaturation at 95°C for 3 min, 10 cycles of denaturation for 20 sec at 98°C, annealing for 15 sec at 65°C and extension for 30 sec at 72°C, followed by a final extension at 72°C for 2 min. Then, 2.5 µL of first PCR product was mixed with the 22.5 µl of second PCR mixture [0.35 µM of primers (i5_2nd, i7-BC_mTrac, and i7-BC_mTrbc), and 1x KAPA Hifi Hotstart ReadyMix], , and the thermal cycling was performed as following condition: denaturation at 95°C for 3 min, 18 cycles of denaturation for 20 sec at 98°C, annealing for 15 sec at 65°C and extension for 30 sec at 72°C, followed by a final extension at 72°C for 2 min.. The second-PCR products were purified by an Agencort AM Pure XP kit (Bexkman-Coulter, CA, #A63881) at a 0.7:1 ratio of beads to sample, and eluted with 20 µL of Tris-HCl (pH 8.0).To amplify TCR library and add adaptor sequence for next generation sequencer, the third PCR was performed as follows. 5µL of purified second PCR product was mixed with the 20 µl of third PCR mixture [0.4 µM of primers (i5-BC and i7-BC), and 1x KAPA Hifi Hotstart ReadyMix (KAPA Biosystems, Wilmington, MA, USA, #KK2602)], and the thermal cycling was performed under the same condition as first PCR, excepted for the number of cycles (23 cycles). The third-PCR products were purified as second PCR. The products were pooled and then purified and subjected to dual size selection using ProNex size-selective purification system (Promega) and eluted with 25 μL of Tris-HCl (pH 8.5). Final TCR libraries, whose lengths were about 600 base pairs were sequenced using an Illumina Novaseq 6000 S4 flowcell (67 bp read 1 and 140 bp read 2) (Illumina, USA). Only read2 contained the sequence regarding the definition of T cell clones. For the single cell library, live cells were stained with Calcein AM (Nacalai) and loaded on an BD Rhapsody cartridge (BD) and processed using BD Rhapsody Cartridge Reagent Kit (BD) following the manufacturer’s instructions. Single-cell cDNA synthesis was performed using BD Rhapsody cDNA Kit (BD) following the manufacturer’s protocol. Whole transcriptome analysis (WTA), TCRseq, and Sample tag Index libraries were prepared using Immune Response Panel Mm (BD) according to the manufacturer’s instructions. Amplified libraries were quantified using a KAPA SYBR Fast qPCR Kit (KAPA Biosystems) and its size distribution was analyzed by a MultiNA. WTA, TCRseq, and Sample tag libraries were sequenced on an Illumina Novaseq 6000 S4 flowcell (67 bp read 1 and 140 bp read 2) using NovaSeq 6000 S4 Reagent Kit v1.5 to a depth of approximately 20,000 reads, 7,000 reads, and 10,000 reads per cell, respectively.

tag: tissue

value: Tumor

tag: cell line

value: Lewis lung carcinoma

tag: cell type

value: lineage-negative (CD11b, B220, NK1.1 and Ter119), TCRb+,  CD4-, CD8+, PD-1+, Ly108+ and Tim3- T cells

tag: genotype

value: WT

tag: time


title: rep2_lipo

city: Cold Spring Harbor

first: Adrian

middle: R

last: Krainer

state: NY

address: 1 Bungtown Rd

country: USA

institute: Cold Spring Harbor Laboratory

data_processing:
Bulk RNA-seq analyses were implemented and integrated using the “CodeSpringLab” platform developed by the CSHL Bioinformatics core, downloaded from https://github.com/RadUtama/CodeSpringLab.git. Quality control of raw sequencing files (fastq) was implemented using FastQC v0.11.8 and FastQ Screen v0.15.2 with default parameters.
Sequence alignment was performed using STAR v2.7.10a with parameters set to ‘--outFilterMismatchNmax-2’, ‘--outFilterMultimapNmax-2’, ‘--outSAMtype BAM SortedByCoordinate’, ‘--outSAMunmapped None’, ‘--outSAMstrandField None’. The human genome reference (FASTA assembly and GTF annotation) was extracted from GENCODE GRCh38.p13 v42. Gene quantification was performed using featureCounts from Subread v2.0.2 with parameters set to ‘-p --countReadPairs’, ‘-t exon’, ‘-s 0’, ‘-Q 12’, ‘-C –minOverlap 1’.
Assembly: GENCODE GRCh38.p13 v42

organism: Homo sapiens

growth_protocol: Cells were used within 10 passages for all experiments, and were confirmed to be Mycoplasma-free routinely during the course of the study, using a Lonza MycoAlert kit. Cells were cultured at 37C and 5% CO2. SNU449 (human HCC) cells were obtained from ATCC and cultured in RPMI 1640 (Corning) supplemented with 10% FBS and 1% penicillin/streptomycin (P/S).

extract_protocol:
RNA isolation was performed using a Direct-zol RNA Miniprep kit (Zymo Research).
RNA-seq libraries were prepared with Kapa mRNA Hyper Prep kit (Roche).

treatment_protocol: For transfection with either siRNA or PKM-ASO in a 6-well plate, 7.5 µL Lipofectamine 3000 (Thermo Fisher Scientific) was mixed with 250 µL opti-MEM (Thermo Fisher Scientific), then mixed with 250 µL opti-MEM containing 10x of the final ASO or siRNA concentration, and incubated for 20 min. 500 µL of the mix was then added dropwise to 2 mL of culture medium without 1% P/S. For Doxycycline treatment, 0.25 ug/ml Dox was added to the media.

value: heptaocellular carcinoma cells

tag: treatment

value: lipofectamine


title: rep1_dox

value: dox


title: ExT6_day14_Tumor_Ly108_2

description: Library name: Sample 7


title: testes of blue catfsh sampled at age 2.9, biological rep3

city: Auburn

first: Ian

last: Butts

state: Alabama

address: 203, Swingle Hall

department: School of Fisheries, Aquaculture & Aquatic Sciences

institute: Auburn University

description: Library name: Seasonal_Sample_3

data_processing:
Raw, demultiplexed sequence files were provided in FASTQ format. The quality of raw RNA sequencing reads was assessed by FastQC (V.0.11.9). Clean reads were obtained by removing low-quality reads and adapter sequences from the raw data using Trimmomatic (V.0.39).
The genome of blue catfish (I. furcatus) was obtained from NCBI and remaining high-quality reads were aligned to the genome using STAR aligner (V.2.7.5). Post mapping quality control was performed using MultiQC (V.1.10.1).
BAM files were used to generate a gene-level count data matrix for all samples via HTSeq-count (V. 0.11.1).
Pairwise differential gene expression analyses were performed using edgeR (V.4.4.2)
Assembly: GCF_023375685.1
Supplementary files format and content: CSV files include raw counts for each sample

molecule: total RNA

organism: Ictalurus furcatus

source_name: Testis

extract_protocol:
Total RNA was extracted using Quick-RNA Miniprep Plus Kit (Zymo Research) following the manufacturer’s instructions. Total RNA was treated with DNase I and resuspended in 50 µL of nuclease-free water.
Sequencing libraries were prepared using a NEBNext Ultra II RNA Library Preparation Kit (NEB, Ipswich, MA, U.S.), following manufacturer’s instructions

value: Testis

tag: Sex

value: male

tag: age

value: Age 2.9


title: Whole blood, KY-0034-10, PETCT2, Normal lung, Hot LN_No, Hot Lung_No

city: Melbourne

first: Dylan

last: Sheerin

state: VIC

address: 1G Royal Parade

country: Australia

institute: WEHI

data_processing:
Forward and reverse reads were then aligned to the reference genome index using the Rsubread align function.
Gene counts were quantified using the BAM files generated from this step, featureCounts (v2.0.3).
Assembly: GRCh38 DNA primary assembly
Supplementary files format and content: .txt formatted gene count matrix

source_name: Whole blood

extract_protocol:
RNA was extracted from thawed Tempus tubes using the PerfectPure™ Blood RNA Kit, including DNAse treatment.
1000 ng of the isolated total RNA was globin reduced and TruSeq Stranded kit used for library preparation with 100bp pair-end (PE100) read chemistry.

value: Whole blood


title: testes of blue catfsh sampled at age 2.9, biological rep2

description: Library name: Seasonal_Sample_2


title: ExT6_day14_Tumor_Ly108_1

description: Library name: Sample 6


title: rep1_si156


title: ExT6_day14_Tumor_PD1nega_1

description: Library name: Sample 11

value: lineage-negative (CD11b, B220, NK1.1 and Ter119), TCRb+,  CD4-, CD8+, PD-1- T cells


title: rep1_tmo2

value: PKM-ASO


title: rep3_ctrl_sirna

value: siCtrl


title: Whole blood, KY-0034-10, PETCT1, Normal lung, Hot LN_No, Hot Lung_No


title: ExT6_day14_Tumor_DP_5

description: Library name: Sample 5

value: lineage-negative (CD11b, B220, NK1.1 and Ter119), TCRb+,  CD4-, CD8+, PD-1+, Ly108+ and Tim3+ T cells


title: Whole blood, KY-0034-07, PETCT2, Normal lung, Hot LN_No, Hot Lung_No


title: rep1_lipo


title: 4Cseq-1h30-control-replicate-FLT3

city: Gif-sur-Yvette

first: Daan

last: Noordermeer

address: Avenue de la Terrasse

country: France

institute: Université Paris-Saclay

description: Library name: 1h30-control-replicate-FLT3

library_source: genomic

data_processing:
Data were processed using c4ctus (https://github.com/NoordermeerLab/c4ctus) using the following primer file: >FLT3|AATTGCCTTGGTTGACATTGAAACCCTGCCTGTTTTTCTTGATC|chr13:27323682-27324757|GATC|Exclude=chr13:27321515-27326762 AATTGCCTTGGTTGACAT
Assembly: T2T CHM13v2.0/hs1
Supplementary files format and content: bedGraph
Library strategy: 4C-Seq

molecule: genomic DNA

source_name: HeLa Kyoto

growth_protocol: For synchronization of HeLa Kyoto cells in the early G1 phase for 4C analysis, 24 h after plating the cells, they were synchronized in prometaphase with 100 nM nocodazole for 17 h. The synchronized cells were collected by mitotic shake-off and washed thrice with 1X PBS to remove nocodazole.

extract_protocol:
10-15 million cells were collected, washed once with 1X PBS, and cross-linked using freshly prepared 2% formaldehyde solution by tumbling for 10 min at room temperature. The fixation was stopped by adding glycine and immediately transferring the tubes to ice. The cross-linked cells were collected at 300g for 5 min at 4℃ and washed once with 1X PBS. After removing the supernatant, the pellet was snap-freeze in liquid nitrogen and stored at -80°C. Chromatin fixation, cell lysis and 4C-seq procedure were done as described in Miranda et al, Methods in Molecular Biology 2022 using 10-15 million cells per cell experiment. MboI (New England Biolabs) was used as the primary restriction enzyme and NlaIII (New England Biolabs) as the secondary restriction enzyme.
4C-seq sequencing libraries using the single-copy FLT3 gene were generated using a 2-step amplification approach, as described in Haarhuis et al, Cell 2017 and Krijger et al, Methods 2020. In the first step, a total of 900ng of 4C material was amplified using the Expand Long Template PCR System (Roche Diagnostics) in 12 reactions in parallel for 15 cycles. All reactions were pooled, followed by clean-up of 1/6th of the material using Agencourt Ampure XP Beads (Beckman Coulter) to remove fragments smaller than 200 bp. In the second step, 20% of the purified PCR products were further amplified in 2 reactions using the Expand Long Template PCR System and universal index adapters for 20 cycles. Amplified 4C-seq libraries were again purified with Agencourt Ampure XP Beads to remove fragments smaller than 200 bp. Quality and size distribution of the PCR libraries was verified by Qubit ds DNA BR kit (Thermo Fisher Scientific) and Tapestation Genomic DNA reagents (Agilent).

treatment_protocol: Cells were plated in media containing DMSO or MLN8237 for 1.5 hrs followed by harvesting for 4C analysis.

value: HeLa Kyoto

value: mitotic shake off, followed by 1.5hr DMSO incubation


title: 4Cseq-1h30-MLN-FLT3

description: Library name: 1h30-MLN-FLT3

value: mitotic shake off, followed by 1.5hr MLN8237 incubation


title: 4Cseq-1h30-MLN-replicate-FLT3

description: Library name: 1h30-MLN-replicate-FLT3


title: rep2_tmo2


title: rep2_ctrl_sirna


title: Whole blood, KY-0034-07, PETCT1, Normal lung, Hot LN_No, Hot Lung_No


title: 4Cseq-1h30-control-FLT3

description: Library name: 1h30-control-FLT3


title: Whole blood, KY-0034-06, PETCT2, Normal lung, Hot LN_No, Hot Lung_No


title: ExT6_day14_Tumor_DP_2

description: Library name: Sample 2

value: Tumor

value: Lewis lung carcinoma


title: ExT6_day14_Tumor_DP_3

description: Library name: Sample 3

value: WT


title: ExT6_day14_Tumor_DP_4

description: Library name: Sample 4


title: 4Cseq-1h30-control-replicate-rDNA

data_processing:
Data were processed using c4ctus (https://github.com/NoordermeerLab/c4ctus) using the following primer file: >rDNA|ATGCAAATGTCAGCCTGGCAAGAATGAGATC|chr14:9662736-9663468|GATC|Exclude=chrW:20-30 ATGCAAATGTCAGCCTGG
Assembly: T2T CHM13v2.0/hs1
Supplementary files format and content: bedGraph
Library strategy: 4C-seq

extract_protocol:
10-15 million cells were collected, washed once with 1X PBS, and cross-linked using freshly prepared 2% formaldehyde solution by tumbling for 10 min at room temperature. The fixation was stopped by adding glycine and immediately transferring the tubes to ice. The cross-linked cells were collected at 300g for 5 min at 4℃ and washed once with 1X PBS. After removing the supernatant, the pellet was snap-freeze in liquid nitrogen and stored at -80°C. Chromatin fixation, cell lysis and 4C-seq procedure were done as described in Miranda et al, Methods in Molecular Biology 2022 using 10-15 million cells per cell experiment. MboI (New England Biolabs) was used as the primary restriction enzyme and NlaIII (New England Biolabs) as the secondary restriction enzyme.
4C-seq sequencing libraries using the rDNA viewpoint, which is present in the human genome in several hundred copies, were generated using a 2-step amplification approach with reduced amounts of input, as described in Haarhuis et al, Cell 2017 and Krijger et al, Methods 2020. In the first step, a total of 120ng of 4C material was amplified using the Expand Long Template PCR System (Roche Diagnostics) in 12 reactions in parallel for 12 cycles. All reactions were pooled, followed by clean-up of 1/6th of the material using Agencourt Ampure XP Beads (Beckman Coulter) to remove fragments smaller than 200 bp. In the second step, 20% of the purified PCR products were further amplified in 2 reactions using the Expand Long Template PCR System and universal index adapters for 20 cycles. Amplified 4C-seq libraries were again purified with Agencourt Ampure XP Beads to remove fragments smaller than 200 bp. Quality and size distribution of the PCR libraries was verified by Qubit ds DNA BR kit (Thermo Fisher Scientific) and Tapestation Genomic DNA reagents (Agilent).


title: 4Cseq-1h30-MLN-replicate-rDNA


title: 4Cseq-1h30-MLN-rDNA


title: Whole blood, KY-0034-06, PETCT1, Normal lung, Hot LN_No, Hot Lung_No


title: 4Cseq-1h30-control-rDNA


title: ExT6_day14_Tumor_DP_1

description: Library name: Sample 1


title: rep1_ctrl_sirna

value: heptaocellular carcinoma cells


title: Whole blood, KY-0034-05, PETCT2, Normal lung, Hot LN_No, Hot Lung_No


title: NOD islet CD45+ cells, 12 weeks, low-dose STZ, biol rep 2

city: St. Louis

first: Pavel

last: Zakharov

state: MO

address: 660 S Euclid Ave Box 8118

department: Pathology and Immunology

institute: Wash.Univ in St. Louis

description: Library name: scRNASeq_NOD_STZ_12w_biol_rep2

library_source: transcriptomic single cell

data_processing:
Downstream filtering, unsupervised clustering, differential expression and plots generation were completed using R and Seurat package. Sample matrices were imported into the Seurat and combined into a Seurat object. Cells were filtered for mitochondrial reads < 5% and for number of reads per cell the thresholds were set to < 300 and not to exceed 3 standard deviations from the mead value: 300 < nCount_RNA < mean(nCount_RNA) + sd(nCount_RNA)*3, where "sd" is standard deviation, and "mean" is mean value. Filtering based on the number of features was done similarly: 300 < nFeature_RNA< mean(nCount_RNA) + sd(nFeature_RNA)*3. Transformation and normalization were performed using SCTransform (v2) to fit a negative binomial distribution and regress out mitochondrial read percentage and number of reads per cell. Principle components (PCs) were calculated, and an elbow plot generated to select the cutoff for number of significant PCs to use for downstream analysis. UMAP dimensionality reduction was then computed using the top 30 PCs. Unsupervised clustering was performed using the "FindNeighbors" and "FindClusters" functions. The differential expression between clusters was performed with "FindAllMarkers" function using "SCT" assay and "data" slot. The DEGs were identified with default Wilcoxon rank-sum method with P. adj < 0.05, log2FC cutoff of 0.25, and minimum percentage of cells expressing an individual gene (min.pct) set to 0.25.
Assembly: mm10
Supplementary files format and content: CellRanger output files: filtered expression matrix, features, and barcodes

source_name: pancreatic islets

extract_protocol:
Mice were first anesthetized with isoflurane and then euthanized by cervical dislocation. Pancreases were harvested, digested in collagenize, and islets isolated by manual handpicking under the Leica DMI1 microscope with P20 pipette into fresh D10 media.
Cells were processed using the Chromium Single Cell 3ʹ Reagent v3 kit from 10X Genomics. A total of 4,000-10,000 cells per sample were used for GEM generation. Reverse transcription, barcoding, cDNA amplification and library preparation were performed per the Chromium 3ʹ v3 protocol at Genome Technology Access Center at the McDonnell Genome Institute (GTAC@MGI). Sequencing was performed on a NovaSeq6000 platform (Illumina) targeting 100,000 150,000 reads per cell or nucleus. Cells were aligned to the mouse GRCm38 transcriptome generated using the CellRanger v6 software (10X Genomics) with "include introns" options set to "False".

treatment_protocol: Mice were injected with single low-dose STZ (or control buffer) at 4 weeks of age, and islets were harvested and analyzed 10 days later (at 5.5 weeks of age)

value: pancreatic islets

tag: strain

value: NOD/ShiLtJ

value: female

value: CD45+ cells

value: single low-dose STZ

tag: age at_the_time_of_treatment

value: 4 weeks

tag: age at_the_time_of_analysis

value: 12 weeks


title: pre3 [RNA-seq]

city: shihezi

first: shiyi

last: li

address: shibei 4th road

country: China

institute: shihezi university

data_processing:
Data analysis: Quality control of raw reads was performed using FastQC (version 0.12.1) , and clean reads were obtained following the removal of low‑quality reads with Trimmomatic (version 0.38) software . The parameter settings used was “HEADCROP:8 LEADING:3 TRAILING:3 SLIDINGWINDOW:4:15 MINLEN:36”. Hisat2 (v2.2.1)  was employed for performing read mapping to Musmusculus genome reference (bosTau9). Gene expression profiling was based on the number of reads. TPM (Transcripts Per Kilobase of exon model per Million mapped reads) values were calculated by StringTie (v2.1.7), and were used to estimate the expressed values. DEGs were obtained using DESeq2 (v1.34.0) with an padj value cutoff < 0.05 and an absolute log2(fold-change) of > 1. KEGG and GO enrichment analysis was performed on the clusterProfiler R package (v4.4.2) .
Assembly: bosTau9
Supplementary files format and content: TPM or readscount values for each sample

organism: Bos taurus

source_name: Blood

extract_protocol:
The RNA-seq libraries were constructed by E-GENE Co. Ltd as follows. Briefly, the total RNA of each sample was extracted using Invitrogen TRIzol® Reagent and then treated with RNase-free DNase I for30 min according to the manufacturer’s protocols. The poly(A) containing mRNA were purified using Oligo(dT) Beads from about 1 ug total RNA.
The captured mRNA was firstly fragmented into 100-200nt using divalent cations at elevated temperature. The fragmented mRNA was reverse transcripted with SuperScript II and then converted to double-stranded cDNA using RNaseH and DNA Pol I by random priming. After purification, the double-stranded cDNA was subjected to blunt-ending, dA addition to 3’-end and adapter ligation.
Finally, PCR was carried out to enrich the adapter ligated cDNA and the libraries were analyzed by Agilent Bioanalyzer 2100 and quantified by qPCR before sequenced by the Illumina sequencing platform.

value: Blood

tag: sample group

value: 30 days post-breeding (Pre30)


title: pre2 [RNA-seq]


title: Whole blood, KY-0034-05, PETCT1, Normal lung, Hot LN_No, Hot Lung_No

value: Whole blood


title: NOD islet CD45+ cells, 12 weeks, low-dose STZ, biol rep 1

description: Library name: scRNASeq_NOD_STZ_12w_biol_rep1


title: Ctrl3 [RNA-seq]

value: non-pregnant cows (Ctrl)


title: Ctrl2 [RNA-seq]


title: NOD islet CD45+ cells, 12 weeks, control, biol rep 2

description: Library name: scRNASeq_NOD_CTRL_12w_biol_rep2

value: control buffer


title: Ctrl1 [RNA-seq]


title: Whole blood, KY-0034-04, PETCT1, Normal lung, Hot LN_No, Hot Lung_No


title: pre2 [RRBS]

data_processing:
Raw data filtration: Adapter sequences were trimmed by using cutadapt. The parameter settings used was “ -a AGATCGGAAGAGC -m 35 -n 2”.
Sequence alignment: The cleaned reads were mapped back to genome using BSMAP software version 2.90 (Xi & Li, 2009). The parameter settings used was “-n 0 -v 0.08 -g 1”. Methylation ratios were extracted from BSMAP output (SAM) using a Python script (methratio.py) which is distributed with the BSMAP package. Only unique mapped reads were used to calculate methylation ratios. Only cytosines in a CpG context with sufficient sequencing depth (greater than or equal to 5x coverage) were retained for further analysis.
DMC detection: Differentially methylated cytosines (DMCs) were detected using MethylKit (v1.20,Akalin A et al.,2012) in de-novo mode among CpG sites with at least 5x coverage. The parameter settings used was “--mincov 5 --m_cutoff 0.1 --q_cutoff 0.05”.
DMR detection: Differentially methylated regions (DMRs) were detected using metilene (Jühling et al., 2016) in de-novo mode among CpG sites with at least 5x coverage. The parameter settings used was “--mincpgs 5 --minMethDiff 0.1 --mtc 1 -X 1 -Y 1 -v 0.7”. Then detected DMR were filtered according to the standard: (1) Q-value must be less than 0.05; (2) methylation level difference must be greater than 0.1; (3) CpG number contained in DMR must be greater than 5; (4) the length of the DMR must be greater than 50bp.
Assembly: bosTau9
Supplementary files format and content: bedgraph,BEDGraph file typically contains information about the methylation status of CpG sites or regions.

extract_protocol:
DNA was extracted from blood samples of pig using a DNA extraction Kit(Beijing, China, Tiangen Biochemical Technology Co., Ltd., DP304-03)
About 1µg of genomic DNA mixed with unmethylated lambda DNA was digested by MspI enzyme for 16 hours at 37℃. After digestion, the libraries were constructed as the Illumina Pair-End protocol with some modifications. Briefly, purified digested DNA was subsequently treated with a mix of T4 DNA polymerase, Klenow Fragment and T4 polynucleotide kinase to repair, blunt and phosphorylate ends. After that, the blunt DNA were 3’ adenylated using Klenow Fragment (3’-5’ exo-) and following with ligation to adaptors synthesized with 5’-methylcytosine instead of cytosine using T4 DNA Ligase. After each step, the DNA was purified using MinElute PCR Purification Kit (Qiagen). ZYMO EZ DNA Methylation-Gold Kit™ was employed to convert unmethylated cytosine into uracil according to the instructions. Finally, PCR was carried out in a final reaction volume of 50µl consisting of 20µl adapter ligated DNA, 4µl 2.5mM dNTP, 5µl 10×buffer, 0.5µl JumpStart™ Taq DNA Polymerase, 2µl PCR primers and 18.5µl water and the following thermal cycling program was 94°C 1 min, 12cycles of 94 °C 10s, 62°C 30s, 72°C 30 then prolong with 5min at 72°C and products could be hold at 12°C.
Before analysis with Illumina sequencing platform, the size selected library was analyzed by the Bioanalyzer analysis system (Agilent, Santa Clara, USA) and quantified by the real time PCR.


title: pre3 [RRBS]


title: pre1 [RRBS]


title: Whole blood, KY-0034-03, PETCT1, Other hot lung lesion, Hot LN_No, Hot Lung_Yes

tag: participant id

tag: initial age


title: NOD islet CD45+ cells, 12 weeks, control, biol rep 1

description: Library name: scRNASeq_NOD_CTRL_12w_biol_rep1


title: Whole blood, KY-0031-04, PETCT1, Other cold lung lesion, Hot LN_No, Hot Lung_No


title: Ctrl1 [RRBS]


title: Ctrl2 [RRBS]


title: Ctrl3 [RRBS]


title: Whole blood, KY-0031-02, PETCT1, Other cold lung lesion, Hot LN_No, Hot Lung_No


title: NOD islet CD45+ cells, 5.5 weeks, low-dose STZ

description: Library name: scRNASeq_NOD_STZ_6w

value: 5.5 weeks


title: Bulk_RNA-seq_Hippocampus, WT, M, n4

city: Sherbrooke

first: Mariano

last: Avino

address: 3201 Jean Mignault

department: Biochemistry and Functional Genomics

country: Canada

institute: Sherbrooke University

description:
Library name: WT-M4
WT-M4

data_processing:
Sequences were trimmed for sequencing adapters and low quality 3' bases using Trimmomatic version 0.35104 and aligned to the reference mouse genome version GRCm38 (gene annotation from Gencode version M23, based on Ensembl 98) using STAR version 2.5.1b105.
Assembly: mouse genome version GRCm38
Supplementary files format and content: CSV file including normalized counts of each sample and each gene

source_name: Hippocampus

extract_protocol:
Total RNA was extracted with TRIzol (Invitrogen) and chloroform (Invitrogen). RNA was then purified with the RNeasy mini kit (Qiagen) according to manufacturer’s instructions.
500ng of total RNA was used for RNA-sequencing libraries preparation. RNA quality control was assessed with the Bioanalyzer RNA 6000 Nano assay on the 2100 Bioanalyzer system (Agilient technologies) and all samples had a RIN above 8. Library preparation was done with the KAPA mRNAseq Hyperprep kit (KAPA, Cat no. KK8581). Ligation was made with 38 nM final concentration of Illumina dual-index UMI (IDT) and 11 PCR cycles was required to amplify cDNA libraries. Libraries were quantified by QuBit and BioAnalyzer DNA1000. All libraries were diluted to 10 nM and normalized by qPCR using the KAPA library quantification kit (KAPA; Cat no. KK4973). Libraries were pooled to equimolar concentration

treatment_protocol: Either of the following diets for 6 months: Control diet ( 70% carbohydrates, 20% long-chain triglycerides, 10% proteins), MCT (70% carbohydrates, 10% medium-chain triglycerides, 10% long-chain triglycerides, 10% proteins), KD (90% long-chain triglycerides, 10% proteins)

value: Hippocampus


title: pre1 [RNA-seq]


title: Bulk_RNA-seq_Hippocampus, WT, M, n3

description:
Library name: WT-M3
WT-M3


title: Bulk_RNA-seq_Hippocampus, WT, M, n2

description:
Library name: WT-M2
WT-M2


title: Whole blood, KY-0031-01, PETCT2, Subclinical TB-inactive, Hot LN_Yes, Hot Lung_Yes


title: Bulk_RNA-seq_Hippocampus, WT, M, n1

description:
Library name: WT-M1
WT-M1


title: Bulk_RNA-seq_Hippocampus, WT, KD, n4

description:
Library name: WT-KD4
WT-K4

value: Carbohydrate-free, high fat ketogenic diet


title: C57BL\6 islet CD45+ cells, control

description: Library name: scRNASeq_C57_CTRL_6w

value: WT


title: Whole blood, KY-0034-04, PETCT2, Normal lung, Hot LN_No, Hot Lung_No


title: Whole blood, KY-0031-01, PETCT1, Subclinical TB-inactive, Hot LN_Yes, Hot Lung_Yes


title: Bulk_RNA-seq_Hippocampus, WT, KD, n3

description:
Library name: WT-KD3
WT-K3


title: Whole blood, KY-0030-04, PETCT2, Subclinical TB, Hot LN_No, Hot Lung_Yes


title: CD4+ T cells plus islet mac STZ biol rep5

description:
Library name: CD4_mac_STZ_biol_rep5
Column name in expression table: stz_5

data_processing:
For the differential analyses, we used DESeq2 package from R/Bioconductor. To control for batch differences, the batch factor (2 batches correspond to 2 independent experiments) was added to the design matrix (~ batch + condition). The Wald test was used to identify differentially expressed genes, and P-values were adjusted with Benjamini-Hochberg method for multiple hypothesis testing. The "apeglm" method was applied for LFC shrinkage15, and the moderated LFC metric was used for pathway analysis and volcano plot. The volcano plot was generated using ‘Enhanced Volcano’ R package. The cutoff for determining significantly differentially expressed genes was an adjusted P-value less than 0.05. For the pathway analysis, genes were ranked according to moderated LFC, and only the top 10,000 genes ordered by mean expression were considered. MSigDB GO:BP gene set collection and "clusterProfiler" R package were used for the pathway analysis. "ComBat-seq" tool ("sva" R package) was used to generate expression table corrected for batch effect, which was only used for PCA and heatmap visualization.
Assembly: GRCm38
Supplementary files format and content: Tab-delimited text files containing raw gene expression counts and batch-corrected, normalized expression values.

source_name: CD4+ T cells from spleen

extract_protocol:
For analysis of CD4+ T cell activation program after co-culturing with islet macrophages, we used viable, single . After co-culture, live CD45+Thy1.2+CD4+ cells were sorted using FACS Aria II (BD Bioscience).
RNA was isolated with the RNeasy Plus Micro Kit (Qiagen). Total RNA integrity (RIN) was determined using Agilent Bioanalyzer or 4200 Tapestation. Library preparation was performed with 10ng of total RNA with a Bioanalyzer RIN score greater than 8.0. ds-cDNA was prepared using the SMARTer Ultra Low RNA kit for Illumina Sequencing (Takara-Clontech) per manufacturer’s protocol. Sequencing was performed on the Illumina NovaSeq6000 S4 XP flow cell with 2x150 paired-end reads, targeting ~30M reads per sample. Dual indexing during library construction was used to uniquely identify each sample. Reads were then aligned to Ensembl GRCm38 primary genome assembly.

treatment_protocol: NOD mice were treated with a single low-dose of streptozotocin (STZ) or control buffer at 4 weeks of age. Islets were harvested 10 days post-injection (at 5.5 weeks of age), and macrophages were subsequently enriched using magnetic beads. Naive CD4+ T cells from BDC2.5 TCR-transgenic mice were co-cultured with islet macrophages for 96 hours.

value: CD4+ T cells from spleen

value: primary naive CD4+ T cells

value: co-culture with islet macrophages from STZ-treated NOD mice

tag: batch

tag: age at_the_time_of_stz_treatment


title: Bulk_RNA-seq_Hippocampus, WT, KD, n2

description:
Library name: WT-KD2
WT-K2

value: female


title: Bulk_RNA-seq_Hippocampus, WT, KD, n1

description:
Library name: WT-KD1
WT-K1


title: CD4+ T cells plus islet mac STZ biol rep4

description:
Library name: CD4_mac_STZ_biol_rep4
Column name in expression table: stz_4

value: NOD/ShiLtJ


title: Bulk_RNA-seq_Hippocampus, WT, Control diet, n3

description:
Library name: WT-C3
WT-C3

value: Control diet


title: Bulk_RNA-seq_Hippocampus, WT, Control diet, n4

description:
Library name: WT-C4
WT-C4


title: Whole blood, KY-0030-04, PETCT1, Subclinical TB, Hot LN_Yes, Hot Lung_Yes


title: Bulk_RNA-seq_Hippocampus, WT, Control diet, n2

description:
Library name: WT-C2
WT-C2


title: NOD islet CD45+ cells, 5.5 weeks, control

description: Library name: scRNASeq_NOD_CTRL_6w

value: pancreatic islets

value: CD45+ cells

value: 4 weeks


title: Whole blood, KY-0030-01, PETCT1, Subclinical TB, Hot LN_Yes, Hot Lung_Yes


title: Bulk_RNA-seq_Hippocampus, 3xTG, M, n4

description:
Library name: 3XTG-M4
3XTG-M4


title: CD4+ T cells plus islet mac STZ biol rep3

description:
Library name: CD4_mac_STZ_biol_rep3
Column name in expression table: stz_3


title: Bulk_RNA-seq_Hippocampus, WT, Control diet, n1

description:
Library name: WT-C1
WT-C1


title: C57BL\6 islet CD45+ cells, low-dose STZ

description: Library name: scRNASeq_C57_STZ_6w

value: single low-dose STZ


title: CD4+ T cells plus islet mac STZ biol rep2

description:
Library name: CD4_mac_STZ_biol_rep2
Column name in expression table: stz_2


title: Bulk_RNA-seq_Hippocampus, 3xTG, M, n1

description:
Library name: 3XTG-M1
3XTG-M1


title: Bulk_RNA-seq_Hippocampus, 3xTG, M, n2

description:
Library name: 3XTG-M2
3XTG-M2


title: Whole blood, KY-0026-01, PETCT2, Normal lung, Hot LN_Yes, Hot Lung_No

value: Whole blood


title: CD4+ T cells plus islet mac STZ biol rep1

description:
Library name: CD4_mac_STZ_biol_rep1
Column name in expression table: stz_1


title: CD4+ T cells plus islet mac control biol rep4

description:
Library name: CD4_mac_control_biol_rep4
Column name in expression table: ctrl_4

value: co-culture with islet macrophages from control NOD mice


title: Whole blood, KY-0030-02, PETCT1, Subclinical TB, Hot LN_Yes, Hot Lung_Yes


title: Bulk_RNA-seq_Hippocampus, 3xTG, M, n3

description:
Library name: 3XTG-M3
3XTG-M3


title: Bulk_RNA-seq_Hippocampus, 3xTG, KD, n4

description:
Library name: 3XTG-KD4
3XTG-K4


title: Whole blood, KY-0029-01, PETCT1, Other cold lung lesion, Hot LN_Yes, Hot Lung_No


title: Bulk_RNA-seq_Hippocampus, 3xTG, KD, n5

description:
Library name: 3XTG-KD5
3XTG-K5


title: Bulk_RNA-seq_Hippocampus, 3xTG, KD, n3

description:
Library name: 3XTG-KD3
3XTG-K3


title: Bulk_RNA-seq_Hippocampus, 3xTG, KD, n2

description:
Library name: 3XTG-KD2
3XTG-K2


title: CD4+ T cells plus islet mac control biol rep3

description:
Library name: CD4_mac_control_biol_rep3
Column name in expression table: ctrl_3


title: Whole blood, KY-0026-01, PETCT1, Normal lung, Hot LN_Yes, Hot Lung_No


title: Whole blood, KY-0024-01, PETCT1, Other cold lung lesion, Hot LN_No, Hot Lung_No


title: CD4+ T cells plus islet mac control biol rep2

description:
Library name: CD4_mac_control_biol_rep2
Column name in expression table: ctrl_2


title: Bulk_RNA-seq_Hippocampus, 3xTG, KD, n1

description:
Library name: 3XTG-KD1
3XTG-K1


title: Whole blood, KY-0022-02, PETCT1, Other cold lung lesion, Hot LN_No, Hot Lung_No


title: CD4+ T cells plus islet mac control biol rep1

description:
Library name: CD4_mac_control_biol_rep1
Column name in expression table: ctrl_1


title: Bulk_RNA-seq_Hippocampus, 3xTG, Control diet, n3

description:
Library name: 3XTG-C3
3XTG-C3


title: Bulk_RNA-seq_Hippocampus, 3xTG, Control diet, n2

description:
Library name: 3XTG-C2
3XTG-C2


title: Bulk_RNA-seq_Hippocampus, 3xTG, Control diet, n1

description:
Library name: 3XTG-C1
3XTG-C1


title: CD4+ T cells alone biol rep 4

description:
Library name: CD4_alone_biol_rep4
Column name in expression table: cd4_alone_4

value: untreated


title: Bulk_RNA-seq_liver, WT, M, n4

data_processing:
Sequences were trimmed for sequencing adapters and low quality 3' bases using Trimmomatic version 0.35104 and aligned to the reference mouse genome version GRCm38 (gene annotation from Gencode version M23, based on Ensembl 98) using STAR version 2.5.1b105.
Assembly: mouse genome version GRCm38
Supplementary files format and content: TSV file includes raw count for each sample

source_name: Liver

extract_protocol:
Total RNA was extracted with TRIzol (Invitrogen) and chloroform (Invitrogen). RNA was then purified with the RNeasy mini kit (Qiagen) according to manufacturer’s instructions.
500ng of total RNA was used for RNA-sequencing libraries preparation. RNA quality control was assessed with the Bioanalyzer RNA 6000 Nano assay on the 2100 Bioanalyzer system (Agilient technologies)and all samples had a RIN above 8. Library preparation was done with the KAPA mRNAseq Hyperprep kit (KAPA, Cat no. KK8581). Ligation was made with 38 nM final concentration of Illumina dual-index UMI (IDT) and 11 PCR cycles was required to amplify cDNA libraries. Libraries were quantified by QuBit and BioAnalyzer DNA1000. All libraries were diluted to 10 nM and normalized by qPCR using the KAPA library quantification kit (KAPA; Cat no. KK4973). Libraries were pooled to equimolar concentration

value: Liver

value: Female


title: CD4+ T cells alone biol rep 3

description:
Library name: CD4_alone_biol_rep3
Column name in expression table: cd4_alone_3


title: Bulk_RNA-seq_liver, WT, M, n3


title: CD4+ T cells alone biol rep 2

description:
Library name: CD4_alone_biol_rep2
Column name in expression table: cd4_alone_2


title: Bulk_RNA-seq_liver, WT, M, n1


title: Bulk_RNA-seq_liver, WT, KD, n3

value: Carbohydrate-free, high fat ketogenic diet


title: CD4+ T cells alone biol rep 1

description:
Library name: CD4_alone_biol_rep1
Column name in expression table: cd4_alone_1


title: Bulk_RNA-seq_liver, WT, KD, n4


title: Whole blood, KY-0016-04, PETCT1, Normal lung, Hot LN_Yes, Hot Lung_No


title: Whole blood, KY-0016-02, PETCT1, Other cold lung lesion, Hot LN_No, Hot Lung_No


title: Bulk_RNA-seq_liver, WT, KD, n1


title: Bulk_RNA-seq_liver, WT, KD, n2


title: Bulk_RNA-seq_liver, WT, Control diet, n3


title: Bulk_RNA-seq_liver, WT, Control diet, n4


title: Whole blood, KY-0018-01, PETCT1, Other hot lung lesion, Hot LN_No, Hot Lung_Yes


title: Whole blood, KY-0015-04, PETCT2, Subclinical TB-inactive, Hot LN_No, Hot Lung_No


title: Whole blood, KY-0015-04, PETCT1, Subclinical TB-inactive, Hot LN_No, Hot Lung_No


title: Bulk_RNA-seq_liver, WT, Control diet, n2


title: Bulk_RNA-seq_liver, WT, Control diet, n1


title: Whole blood, KY-0015-02, PETCT1, Subclinical TB, Hot LN_No, Hot Lung_Yes


title: TRPV1CreDTA, Replicate 2

city: Boston

first: Victor

last: Fattori

state: MA

address: 1 Blackfan Circle, Karp Family Research Building, RB10006C

institute: Boston Children's Hospital

description: 10X Genomics

data_processing:
The raw single-cell RNA sequencing data was preprocessed using CellRanger v7.1.0 (10x Genomics), including aligning reads to the mouse mm10 reference genome, and generating cellxgene expression count matrices
Assembly: mm10
Supplementary files format and content: Tab-separated values files and matrix files

source_name: Peritoneal wash at 28 dpi

extract_protocol:
PerC washes from sham littermate controls and lesion-bearing TRPV1-creDTA and littermate control mice were collected at 28dpi and used for droplet-based scRNA-seq (10x Genomics).
Library preparation was conducted using the Next GEM Single Cell 3ʹ Reagent Kits v3.1 Dual Index Kit TT, Set A (10x Genomics) following the manufacturer’s instructions

value: Peritoneal wash at 28 dpi

value: Peritoneal wash immune cells

value: TRPV1cre+DTA+ female mice


title: Bulk_RNA-seq_liver, 3xTG, M, n4


title: Whole blood, KY-0018-03, PETCT1, Subclinical TB-inactive, Hot LN_Yes, Hot Lung_Yes


title: Bulk_RNA-seq_liver, WT, M, n2


title: WT

city: 北京市

first: 震

last: 张

address: 右安门西头条10号

institute: 首都医科大学

description: Library name: sample 1

data_processing:
Libraries were sequenced on an Illumina NovaSeq 6000 with paired-end 150 bp reads and dual indexing. Minimum depth: 20,000 read pairs/cell.Raw reads were processed using Cell Ranger v7.0.1 (10x Genomics) with default parameters. Reads were aligned to the mouse genome (GRCm38) using STAR. Gene-barcode matrices were generated by Cell Ranger.Downstream analysis was performed using Seurat v3.1.1. Cells were filtered based on UMI count, gene number, and mitochondrial content.Dimensionality reduction, clustering (PCA, UMAP), and cell type annotation were performed using Seurat functions.
Assembly: GRCm38 (mm10)
Supplementary files format and content: Processed data include filtered gene expression matrices in Matrix Market format (matrix.mtx.gz, barcodes.tsv.gz, features.tsv.gz) generated by Cell Ranger.

source_name: brain

growth_protocol: Eighteen-month-old C57BL/6J wild-type or CD200R1⁻/⁻ mice were used. Animals were housed under SPF conditions with standard chow and 12-hour light/dark cycle.

extract_protocol:
Mice were transcardially perfused with ice-cold PBS. Brains were dissected and dissociated using the Adult Brain Dissociation Kit (Miltenyi Biotec) and gentleMACS Octo Dissociator. CD11b⁺ cells were isolated using BD IMag magnetic particles.
Single-cell suspensions were processed using Chromium Next GEM Single Cell 3′ Reagent Kit v3.1 (10x Genomics, Cat# PN-1000268) and Chip G on a Chromium Controller. GEMs were generated, followed by reverse transcription, cDNA purification, amplification, and library preparation according to 10x Genomics protocol (CG000315 Rev E).

treatment_protocol: Mice received no treatment prior to tissue collection.

value: brain


title: Bulk_RNA-seq_liver, 3xTG, M, n3


title: KO

description: Library name: sample 2


title: Endo, Replicate 2

description: 10X Genomics

value: TRPV1cre+DTA- female mice


title: Rat, Unperfused, Replicate 2

city: Hamburg

first: Fabian

last: Hausmann

address: Falkenried 94

department: Institute of Medical Systems Biology

country: Germany

institute: University Medical Center Hamburg-Eppendorf

data_processing:
Assembly: mRatBN7.2 (GCA_015227675.2)
Cell Ranger alignment (v.5.0.1)
Supplementary files format and content: Filtered feature bc matrix as produced by cellranger

molecule: nuclear RNA

organism: Rattus norvegicus

source_name: Kidney

extract_protocol:
A snap frozen kidney tissue sample from pig, mouse, or rat was thawed on ice and 3-4 mm of the core was chopped with a razor blade in a petri dish on ice and homogenized using a Dounce homogenizer (D8938-1 SET, Sigma-Aldrich) in 200 µl ice-cold lysis solution and incubated on ice for 20-30 min with additional 3.8 ml of ice-cold lysis solution. Lysis solution was prepared with Nuclei PURE lysis buffer (NUC-201, Sigma-Aldrich), 1 mM dithiothreitol (D9779, Sigma-Aldrich) and 0.1 % Triton X-100 (NUC-201, Sigma-Aldrich) according to manufacturer protocol and an RNAse inhibitor mix (0.04 U/µl SUPERaseIN RNAse Inhibitor [AM 2696, Thermo Fisher]; 0.04 U/µl RNAsin Plus RNAse Inhibitor [N2615, Promega]) was added. The single nuclei suspension was filtered through a 30 µm strainer (04-004-2326, Sysmex) and centrifuged at 500g for 5 min at 4°C. The pellet obtained from dissociated rat kidney tissue was resuspended in 2% BSA in DPBS with 0.04 U/µl SUPERaseIN RNAse Inhibitor and 0.04 U/µl RNAsin Plus RNAse Inhibitor and nuclei were counted. The pellet obtained from dissociated mouse and pig kidney tissue was resuspended and incubated for 2 min in 1ml red blood cell lysis buffer hybri-max™ (R7757-100ml, Sigma Aldrich), filtered through a 5 µm strainer (04-004-2323, Sysmex) and washed with 4 ml of ice-cold 0,01% BSA (AM2616, Thermo Fisher) in DPBS (59331C; Sigma) with 0.04 U/µl SUPERaseIN RNAse Inhibitor and 0.04 U/µl RNAsin Plus RNAse Inhibitor at 500 g for 5 min at 4°C. The pellet was resuspended in 1% BSA (mouse and pig) in DPBS with 0.04 U/µl SUPERaseIN RNAse Inhibitor and 0.04 U/µl RNAsin Plus RNAse Inhibitor and nuclei were counted.
Nuclei number was counted and samples were diluted before loading to the Chromium 10X device to capture ~ 10 000 nuclei. The nuclei were separated into Gel Bead Emulsion droplets and libraries were prepared with the Chromium NEXT GEM Single Cell 3’ Reagent kits v3.1 according to manufacturer's protocol.

value: Kidney

value: Unperfused


title: Bulk_RNA-seq_liver, 3xTG, M, n2


title: Whole blood, KY-0007-04, PETCT1, Subclinical TB-inactive, Hot LN_No, Hot Lung_Yes


title: iPSC-derived endothelial cells, condition 3, replicate 3

city: Basel

first: Martina

last: Pigoni

address: Grenzacherstrasse 124

department: NRD

country: Switzerland

institute: Hoffmann-La Roche

description: Library name: MP22-057-con-3-01-12-2022

data_processing:
Base calling was conducted using the BCL to FASTQ file converter bcl2fastq2 version 2.20.0 (Illumina).
Quality assessment of FASTQ files was performed with FastQC version 0.12.1 (Andrews et al. 2010). Paired-end reads were aligned to the human genome (build "hg38") using the STAR read aligner version 2.7.11b with default mapping parameters (Dobin et al. 2013).
Alignment metrics were determined using Picard version 3.1.1 (Broad Institute).
Quality of read sequences and alignments was assessed with MultiQC version 1.21.
The number of reads mapped to all RefSeq transcript variants of a gene were combined into a single count value (i.e., read count) assuming a reverse-stranded library, using featureCounts version 2.0.6.
Assembly: hg38
Supplementary files format and content: raw count matrix using RefSeq gene IDs, GCT file format
Supplementary files format and content: log2tpm per gene using RefSeq gene IDs, GCT file format

source_name: iPSC-derived endothelial cells

extract_protocol:
300,000 cells differentiated to iECs (cond 1), iEC-rep (cond 2), iCE-BECs (cond 3) were plated in 6 well plates at day 11 and grown at confluence to day 14. At day 14, cells were collected and pellets were snap frozen.
RNA was extracted and preparation of Illumina stranded TruSeq RNA libraries, including poly(A) enrichment, was performed (2*100 bp paired-end reads).

value: iPSC-derived endothelial cells

value: condition 3


title: Rat, Perfused, Replicate 3

value: Perfused


title: Bulk_RNA-seq_liver, 3xTG, KD, n4


title: Bulk_RNA-seq_liver, 3xTG, M, n1


title: Rat, Unperfused, Replicate 1


title: Sham, Replicate 2

description: 10X Genomics

value: TRPV1cre-DTA+ female mice


title: Endo, Replicate 1

description: 10X Genomics


title: iPSC-derived endothelial cells, condition 1, replicate 3

description: Library name: MP22-057-con-1-01-12-2022

value: condition 1


title: iPSC-derived endothelial cells, condition 2, replicate 3

description: Library name: MP22-057-con-2-01-12-2022

value: condition 2


title: Rat, Perfused, Replicate 2


title: Sham, Replicate 1

description: 10X Genomics


title: TRPV1CreDTA, Replicate 1

description: 10X Genomics


title: Bulk_RNA-seq_liver, 3xTG, KD, n3


title: Pig, Perfused, Replicate 2

data_processing:
Assembly: Sscrofa11.1 (GCA_000003025.6)
Cell Ranger alignment (v.5.0.1)
Supplementary files format and content: Filtered feature bc matrix as produced by cellranger

organism: Sus scrofa


title: Mouse, Perfused, Replicate 3

data_processing:
Assembly: mm10-2020-A
Cell Ranger alignment (v.5.0.1)
Supplementary files format and content: Filtered feature bc matrix as produced by cellranger


title: Mouse, Perfused, Replicate 2


title: iPSC-derived endothelial cells, condition 3, replicate 2

description: Library name: MP22-050-con-3-03-11-2022


title: Bulk_RNA-seq_liver, 3xTG, KD, n2


title: Mouse, Perfused, Replicate 1


title: Rat, Unperfused, Replicate 3


title: Bulk_RNA-seq_liver, 3xTG, Control diet, n4

value: Control diet


title: Whole blood, KY-0007-03, PETCT1, Other cold lung lesion, Hot LN_No, Hot Lung_No

value: Whole blood


title: iPSC-derived endothelial cells, condition 1, replicate 2

description: Library name: MP22-050-con-1-03-11-2022


title: Bulk_RNA-seq_liver, 3xTG, KD, n1

value: Liver

value: Female


title: iPSC-derived endothelial cells, condition 2, replicate 2

description: Library name: MP22-050-con-2-03-11-2022


title: Mouse, Unperfused, Replicate 1


title: Bulk_RNA-seq_liver, 3xTG, Control diet, n2


title: Mouse, Unperfused, Replicate 2


title: Pig, Unperfused, Replicate 1


title: Rat, Perfused, Replicate 1


title: Bulk_RNA-seq_liver, 3xTG, Control diet, n3


title: Pig, Unperfused, Replicate 2


title: Bulk_RNA-seq_liver, 3xTG, Control diet, n1


title: iPSC-derived endothelial cells, condition 1, replicate 1

description: Library name: MP22-045-con-1-13-10-2022


title: bone_marrow_reads

city: Ann Arbor

first: Lindsey

last: Muir

state: Michigan

address: 1600 Huron Parkway

institute: University of Michigan

description: Library name: bone_marrow

data_processing:
Raw reads were processed with EPI2ME Labs `wf-single-cell' (https://github.com/epi2me-labs/wf-single-cell)
Assembly: GRCh38
Supplementary files format and content: Processed files are scanpy compliant AnnData objects.

source_name: bone marrow

growth_protocol: All cells were cultured at 37°C with 5% CO₂. Neonatal human foreskin fibroblasts (BJ, ATCC CRL-2522, RRID:CVCL_3653) were used as the initial cell type for reprogramming. Cells used for control and reprogramming cultures were at an initial passage number between 2 and 4, and were at 10 or fewer passages for endpoints. Fibroblasts were cultured in high glucose DMEM containing sodium pyruvate + 10% fetal bovine serum + 1% penicillin/streptomycin (P/S) + 1% L-glutamine. For reprogramming cultures, plates were coated with 0.1% porcine gelatin (Millipore #SF008). Primary human bone marrow CD34+ cells (ATCC PCS-800-012TM) were cultured in complete MyeloCult H5100 (STEMCELL Technologies 05150).

extract_protocol:
Adherent cells were dissociated using 1x TrypLE Express Enzyme (Gibco #12604-021)
Full-length barcoded cDNA amplicons were obtained from single cells using the 10X Genomics Next GEM Single Cell 3' Kit V3.1. Briefly, 20,000 sorted CD34+ cells and 2,000 control fibroblasts were loaded onto the 10X Genomics Chromium Controller (X) to partition single cells into Gel Beads-In Emulsion (GEMs). cDNA was generated from polyadenylated mRNA transcripts, incorporating a unique molecular identifier (UMI) and a cell-specific barcode for each transcript. Barcoded cDNA was pooled, purified with SILANE magnetic beads (10X Genomics #PN-2000048), and amplified by PCR. Barcoded cDNA libraries were prepared for sequencing according to the Oxford Nanopore Technologies (ONT) 3' cDNA protocols. Briefly, cDNA amplicons were biotin tagged by PCR with the primers shown in the ONT protocols. Biotinylated cDNA was purified with Dynabeads™ M-280 Streptavidin beads (Invitrogen #11205D), followed by secondary PCR amplification and Agencourt AMPure XP bead clean-up (Beckman Coulter #A63881). Products were assessed for quality following ONT recommendations. Long-read cDNA libraries were prepared for sequencing as in ONT protocols and sequenced on an ONT GridION or PromethION Solo 2 (P2) sequencer. Raw reads were base-called with guppy using the High Accuracy basecalling model and stored as fastq files.

treatment_protocol: Lentiviral transductions were carried out in complete medium without penicillin/streptomycin in the presence of 8 µg/mL polybrene (Millipore Sigma TR-1003-G). Integrated TF expression cassettes were induced with 1 µg/mL doxycycline hyclate (Dox, Millipore Sigma D9891) supplemented in the culture medium for 48 hours. Control fibroblasts were untransduced for single cell transcriptomics experiments. Reprogramming cells were switched to complete MyeloCult H5100 with 10e-6 M hydrocortisone (Millipore Sigma #H0888) and 1% P/S for 48 hours after the initial Dox treatment.

value: bone marrow

value: various


title: Pig, Perfused, Replicate 1


title: SVZ NSC lineage cell, OB rep3 [OB3_592844]

status: Public on Aug 08 2025

city: Heidelberg

first: Leo

middle: Carl

last: Förster

state: Deutschland

address: Im Neuenheimer Feld 581

institute: DKFZ

data_processing:
Read1 sequences were processed to extract and trim UMIs, storing them in the FASTQ identifier field. Processed paired-end reads were trimmed using trim-galore v0.5.0 and mapped to the mm10 genome reference with Ensembl96 gene annotations using STAR v2.5.3a setting "--bamRemoveDuplicatesType UniqueIdentical --outFilterMismatchNmax 9999 --outFilterScoreMinOverLread 0.2 --outFilterMatchNminOverLread 0.2 --alignSJoverhangMin 3 --alignSplicedMateMapLminOverLmate 0.2 --twopassMode Basic". UMI-containing readpairs were quantified with a custom script based on HTSeq to identify exonic and intronic UMIs and deduplicate them, reporting a merged UMI-counts matrix.  Cell transcriptomes were subset to 1:1 human-mouse orthologs reported in Ensembl102 to be consistent with our downstream human analyses. We excluded cells with fewer than 2000 UMIs or under 500 detected genes.
Assembly: mm10
Supplementary files format and content: counts: comma separated matrix of deduplicated UMI counts per cell (columns) per gene (rows). Metadata: comma separated matrix relating metdata (columns) per cell (rows)

source_name: Brain, olfactory bulb

extract_protocol:
RNA molecules were captured using an oligo dT(30) primer. mRNA was reverse-transcribed using a template switching oligonucleotide contianing an 8 bp unique molecular identifier to obtain cDNA which was subsequently amplified and purified using AMPureXP beads.
Purified cDNA was then used for Illumina Nextera XP library preparation according to Hagemann-Jensen et al. 2020 with modifications reported in Cerrizuela and Kaya et al. 2022.

value: Brain, olfactory bulb


title: SVZ NSC lineage cell, OB rep3 [OB3_592842]


title: SVZ NSC lineage cell, OB rep3 [OB3_592848]


title: SVZ NSC lineage cell, OB rep3 [OB3_592846]


title: SVZ NSC lineage cell, OB rep3 [OB3_592838]


title: SVZ NSC lineage cell, OB rep3 [OB3_592840]


title: SVZ NSC lineage cell, OB rep3 [OB3_592836]


title: SVZ NSC lineage cell, OB rep3 [OB3_592834]


title: SVZ NSC lineage cell, OB rep3 [OB3_592826]


title: SVZ NSC lineage cell, OB rep3 [OB3_592824]


title: SVZ NSC lineage cell, OB rep3 [OB3_592822]


title: iPSC-derived endothelial cells, condition 3, replicate 1

description: Library name: MP22-045-con-3-13-10-2022


title: SVZ NSC lineage cell, OB rep3 [OB3_592820]


title: Mouse, Unperfused, Replicate 3


title: iPSC-derived endothelial cells, condition 2, replicate 1

description: Library name: MP22-045-con-2-13-10-2022


title: SVZ NSC lineage cell, OB rep3 [OB3_592818]


title: SVZ NSC lineage cell, OB rep3 [OB3_592816]


title: SVZ NSC lineage cell, OB rep3 [OB3_592810]


title: SVZ NSC lineage cell, OB rep3 [OB3_592806]


title: SVZ NSC lineage cell, OB rep3 [OB3_592802]


title: SVZ NSC lineage cell, OB rep3 [OB3_592814]


title: SVZ NSC lineage cell, OB rep3 [OB3_592798]


title: SVZ NSC lineage cell, OB rep3 [OB3_592796]


title: SVZ NSC lineage cell, OB rep3 [OB3_592830]


title: SVZ NSC lineage cell, OB rep3 [OB3_592832]


title: SVZ NSC lineage cell, OB rep3 [OB3_592794]


title: SVZ NSC lineage cell, OB rep3 [OB3_592784]


title: SVZ NSC lineage cell, OB rep3 [OB3_592776]


title: SVZ NSC lineage cell, OB rep3 [OB3_592792]


title: SVZ NSC lineage cell, OB rep3 [OB3_592788]


title: SVZ NSC lineage cell, OB rep3 [OB3_592782]


title: SVZ NSC lineage cell, OB rep3 [OB3_592790]


title: SVZ NSC lineage cell, OB rep3 [OB3_592778]


title: SVZ NSC lineage cell, OB rep3 [OB3_592786]


title: SVZ NSC lineage cell, OB rep3 [OB3_592774]


title: SVZ NSC lineage cell, OB rep3 [OB3_592772]


title: SVZ NSC lineage cell, OB rep3 [OB3_592766]


title: SVZ NSC lineage cell, OB rep3 [OB3_592768]


title: SVZ NSC lineage cell, OB rep3 [OB3_592764]


title: SVZ NSC lineage cell, OB rep3 [OB3_592760]


title: SVZ NSC lineage cell, OB rep3 [OB3_592758]


title: SVZ NSC lineage cell, OB rep3 [OB3_592756]


title: SVZ NSC lineage cell, OB rep3 [OB3_592812]


title: SVZ NSC lineage cell, OB rep3 [OB3_592804]


title: SVZ NSC lineage cell, OB rep3 [OB3_592754]


title: SVZ NSC lineage cell, OB rep3 [OB3_592808]


title: SVZ NSC lineage cell, OB rep3 [OB3_592750]


title: SVZ NSC lineage cell, OB rep3 [OB3_592752]


title: SVZ NSC lineage cell, OB rep3 [OB3_592748]


title: SVZ NSC lineage cell, OB rep3 [OB3_592744]


title: SVZ NSC lineage cell, OB rep3 [OB3_592746]


title: SVZ NSC lineage cell, OB rep3 [OB3_592742]


title: SVZ NSC lineage cell, OB rep3 [OB3_592762]


title: SVZ NSC lineage cell, OB rep3 [OB3_592734]


title: SVZ NSC lineage cell, OB rep3 [OB3_592736]


title: SVZ NSC lineage cell, OB rep3 [OB3_592732]


title: SVZ NSC lineage cell, OB rep3 [OB3_592770]


title: SVZ NSC lineage cell, OB rep3 [OB3_592730]


title: SVZ NSC lineage cell, OB rep3 [OB3_592728]


title: SVZ NSC lineage cell, OB rep3 [OB3_592726]


title: SVZ NSC lineage cell, OB rep3 [OB3_592722]


title: SVZ NSC lineage cell, OB rep3 [OB3_592718]


title: SVZ NSC lineage cell, OB rep3 [OB3_592724]


title: SVZ NSC lineage cell, OB rep3 [OB3_592716]


title: SVZ NSC lineage cell, OB rep3 [OB3_592714]


title: SVZ NSC lineage cell, OB rep3 [OB3_592712]


title: SVZ NSC lineage cell, OB rep3 [OB3_592706]


title: SVZ NSC lineage cell, OB rep3 [OB3_592702]


title: SVZ NSC lineage cell, OB rep3 [OB3_592740]


title: SVZ NSC lineage cell, OB rep3 [OB3_592700]


title: SVZ NSC lineage cell, OB rep3 [OB3_592708]


title: SVZ NSC lineage cell, OB rep3 [OB3_592698]


title: SVZ NSC lineage cell, OB rep3 [OB3_592710]


title: SVZ NSC lineage cell, OB rep3 [OB3_592690]


title: SVZ NSC lineage cell, OB rep3 [OB3_592688]


title: SVZ NSC lineage cell, OB rep3 [OB3_592738]


title: SVZ NSC lineage cell, OB rep3 [OB3_592684]


title: SVZ NSC lineage cell, OB rep3 [OB3_592692]


title: SVZ NSC lineage cell, OB rep3 [OB3_592680]


title: SVZ NSC lineage cell, OB rep3 [OB3_592678]


title: SVZ NSC lineage cell, OB rep3 [OB3_592672]


title: SVZ NSC lineage cell, OB rep3 [OB3_592674]


title: SVZ NSC lineage cell, OB rep3 [OB3_592668]


title: SVZ NSC lineage cell, OB rep3 [OB3_592676]


title: SVZ NSC lineage cell, OB rep3 [OB3_592664]


title: SVZ NSC lineage cell, OB rep3 [OB3_592670]


title: SVZ NSC lineage cell, OB rep3 [OB3_592720]


title: SVZ NSC lineage cell, OB rep3 [OB3_592666]


title: SVZ NSC lineage cell, OB rep3 [OB3_592660]


title: SVZ NSC lineage cell, OB rep3 [OB3_592656]


title: SVZ NSC lineage cell, OB rep3 [OB3_592662]


title: SVZ NSC lineage cell, OB rep3 [OB3_592650]


title: SVZ NSC lineage cell, OB rep3 [OB3_592652]


title: SVZ NSC lineage cell, OB rep3 [OB3_592648]


title: SVZ NSC lineage cell, OB rep3 [OB3_592644]


title: SVZ NSC lineage cell, OB rep3 [OB3_592646]


title: SVZ NSC lineage cell, OB rep3 [OB3_592642]


title: SVZ NSC lineage cell, OB rep3 [OB3_592640]


title: SVZ NSC lineage cell, OB rep3 [OB3_592636]


title: SVZ NSC lineage cell, OB rep3 [OB3_592638]


title: SVZ NSC lineage cell, OB rep3 [OB3_592624]


title: SVZ NSC lineage cell, OB rep3 [OB3_592626]


title: SVZ NSC lineage cell, OB rep3 [OB3_592634]


title: SVZ NSC lineage cell, OB rep3 [OB3_592620]


title: SVZ NSC lineage cell, OB rep3 [OB3_592630]


title: SVZ NSC lineage cell, OB rep3 [OB3_592618]


title: SVZ NSC lineage cell, OB rep3 [OB3_592616]


title: SVZ NSC lineage cell, OB rep3 [OB3_592614]


title: SVZ NSC lineage cell, OB rep3 [OB3_592608]


title: SVZ NSC lineage cell, OB rep3 [OB3_592600]


title: SVZ NSC lineage cell, OB rep3 [OB3_592598]


title: SVZ NSC lineage cell, OB rep3 [OB3_592596]


title: SVZ NSC lineage cell, OB rep3 [OB3_592606]


title: SVZ NSC lineage cell, OB rep3 [OB3_592602]


title: SVZ NSC lineage cell, OB rep3 [OB3_592594]


title: SVZ NSC lineage cell, OB rep3 [OB3_592590]


title: SVZ NSC lineage cell, OB rep3 [OB3_592694]


title: SVZ NSC lineage cell, OB rep3 [OB3_592582]


title: SVZ NSC lineage cell, OB rep3 [OB3_592588]


title: SVZ NSC lineage cell, OB rep3 [OB3_592586]


title: SVZ NSC lineage cell, OB rep3 [OB3_592584]


title: SVZ NSC lineage cell, OB rep3 [OB3_592578]


title: SVZ NSC lineage cell, OB rep3 [OB3_592576]


title: SVZ NSC lineage cell, OB rep3 [OB3_592566]


title: SVZ NSC lineage cell, OB rep3 [OB3_592628]


title: SVZ NSC lineage cell, OB rep3 [OB3_592580]


title: SVZ NSC lineage cell, OB rep3 [OB3_592538]


title: SVZ NSC lineage cell, OB rep3 [OB3_592562]


title: SVZ NSC lineage cell, OB rep3 [OB3_592522]


title: SVZ NSC lineage cell, OB rep3 [OB3_592610]


title: SVZ NSC lineage cell, OB rep3 [OB3_592564]


title: SVZ NSC lineage cell, OB rep3 [OB3_592502]


title: SVZ NSC lineage cell, OB rep3 [OB3_592518]


title: SVZ NSC lineage cell, OB rep3 [OB3_592512]


title: SVZ NSC lineage cell, OB rep3 [OB3_592510]


title: SVZ NSC lineage cell, OB rep3 [OB3_592514]


title: SVZ NSC lineage cell, OB rep3 [OB3_592530]


title: SVZ NSC lineage cell, OB rep3 [OB3_592554]


title: SVZ NSC lineage cell, OB rep3 [OB3_592520]


title: SVZ NSC lineage cell, OB rep3 [OB3_592550]


title: SVZ NSC lineage cell, OB rep3 [OB3_592496]


title: SVZ NSC lineage cell, OB rep3 [OB3_592658]


title: SVZ NSC lineage cell, OB rep3 [OB3_592490]


title: SVZ NSC lineage cell, OB rep3 [OB3_592492]


title: SVZ NSC lineage cell, OB rep3 [OB3_592488]


title: SVZ NSC lineage cell, OB rep3 [OB3_592480]


title: SVZ NSC lineage cell, OB rep3 [OB3_592494]


title: SVZ NSC lineage cell, OB rep3 [OB3_592574]


title: SVZ NSC lineage cell, OB rep3 [OB3_592572]


title: SVZ NSC lineage cell, OB rep3 [OB3_592486]


title: SVZ NSC lineage cell, OB rep3 [OB3_592524]


title: SVZ NSC lineage cell, OB rep3 [OB3_592504]


title: SVZ NSC lineage cell, OB rep3 [OB3_592478]


title: SVZ NSC lineage cell, OB rep3 [OB3_592508]


title: SVZ NSC lineage cell, OB rep3 [OB3_592474]


title: SVZ NSC lineage cell, OB rep3 [OB3_592464]


title: SVZ NSC lineage cell, OB rep3 [OB3_592476]


title: SVZ NSC lineage cell, OB rep3 [OB3_592470]


title: SVZ NSC lineage cell, OB rep3 [OB3_592500]


title: SVZ NSC lineage cell, OB rep3 [OB3_592466]


title: SVZ NSC lineage cell, OB rep3 [OB3_592462]


title: SVZ NSC lineage cell, OB rep3 [OB3_592460]


title: SVZ NSC lineage cell, OB rep3 [OB3_592456]


title: SVZ NSC lineage cell, OB rep3 [OB3_592442]


title: SVZ NSC lineage cell, OB rep3 [OB3_592444]


title: SVZ NSC lineage cell, OB rep3 [OB3_592436]


title: SVZ NSC lineage cell, OB rep3 [OB3_592440]


title: SVZ NSC lineage cell, OB rep3 [OB3_592454]


title: SVZ NSC lineage cell, OB rep3 [OB3_592452]


title: SVZ NSC lineage cell, OB rep3 [OB3_592458]


title: SVZ NSC lineage cell, OB rep3 [OB3_592430]


title: SVZ NSC lineage cell, OB rep3 [OB3_592428]


title: SVZ NSC lineage cell, OB rep3 [OB3_592438]


title: SVZ NSC lineage cell, OB rep3 [OB3_592434]


title: SVZ NSC lineage cell, OB rep3 [OB3_592432]


title: SVZ NSC lineage cell, OB rep3 [OB3_592424]


title: SVZ NSC lineage cell, OB rep3 [OB3_592450]


title: SVZ NSC lineage cell, OB rep3 [OB3_592472]


title: SVZ NSC lineage cell, OB rep3 [OB3_592420]


title: SVZ NSC lineage cell, OB rep3 [OB3_592426]


title: SVZ NSC lineage cell, OB rep3 [OB3_592422]


title: SVZ NSC lineage cell, OB rep3 [OB3_592406]


title: SVZ NSC lineage cell, OB rep3 [OB3_592468]


title: SVZ NSC lineage cell, OB rep3 [OB3_592398]


title: SVZ NSC lineage cell, OB rep3 [OB3_592412]


title: SVZ NSC lineage cell, OB rep3 [OB3_592416]


title: SVZ NSC lineage cell, OB rep3 [OB3_592408]


title: SVZ NSC lineage cell, OB rep3 [OB3_592402]


title: SVZ NSC lineage cell, OB rep3 [OB3_592400]


title: SVZ NSC lineage cell, OB rep3 [OB3_592414]


title: SVZ NSC lineage cell, OB rep3 [OB3_592506]


title: SVZ NSC lineage cell, OB rep3 [OB3_592390]


title: SVZ NSC lineage cell, OB rep3 [OB3_592388]


title: SVZ NSC lineage cell, OB rep3 [OB3_592384]


title: SVZ NSC lineage cell, OB rep3 [OB3_592380]


title: SVZ NSC lineage cell, OB rep3 [OB3_592386]


title: SVZ NSC lineage cell, OB rep3 [OB3_592376]


title: SVZ NSC lineage cell, OB rep3 [OB3_592374]


title: SVZ NSC lineage cell, OB rep3 [OB3_592378]


title: SVZ NSC lineage cell, OB rep3 [OB3_592372]


title: SVZ NSC lineage cell, OB rep3 [OB3_592370]


title: SVZ NSC lineage cell, OB rep3 [OB3_592366]


title: SVZ NSC lineage cell, OB rep3 [OB3_592396]


title: SVZ NSC lineage cell, OB rep3 [OB3_592364]


title: SVZ NSC lineage cell, OB rep3 [OB3_592360]


title: SVZ NSC lineage cell, OB rep3 [OB3_592368]


title: SVZ NSC lineage cell, OB rep3 [OB3_592362]


title: SVZ NSC lineage cell, OB rep3 [OB3_592356]


title: SVZ NSC lineage cell, OB rep3 [OB3_592348]


title: SVZ NSC lineage cell, OB rep3 [OB3_592358]


title: SVZ NSC lineage cell, OB rep3 [OB3_592346]


title: SVZ NSC lineage cell, OB rep3 [OB3_592338]


title: SVZ NSC lineage cell, OB rep3 [OB3_592336]


title: SVZ NSC lineage cell, OB rep3 [OB3_592350]


title: SVZ NSC lineage cell, OB rep3 [OB3_592392]


title: SVZ NSC lineage cell, OB rep3 [OB3_592340]


title: SVZ NSC lineage cell, OB rep3 [OB3_592332]


title: SVZ NSC lineage cell, OB rep3 [OB3_592328]


title: SVZ NSC lineage cell, OB rep3 [OB3_592334]


title: SVZ NSC lineage cell, OB rep3 [OB3_592330]


title: SVZ NSC lineage cell, OB rep3 [OB3_592320]


title: SVZ NSC lineage cell, OB rep3 [OB3_592306]


title: SVZ NSC lineage cell, OB rep3 [OB3_592324]


title: SVZ NSC lineage cell, OB rep3 [OB3_592312]


title: SVZ NSC lineage cell, OB rep3 [OB3_592308]


title: SVZ NSC lineage cell, OB rep3 [OB3_592302]


title: SVZ NSC lineage cell, OB rep3 [OB3_592298]


title: SVZ NSC lineage cell, OB rep3 [OB3_592292]


title: SVZ NSC lineage cell, OB rep3 [OB3_592294]


title: SVZ NSC lineage cell, OB rep3 [OB3_592274]


title: SVZ NSC lineage cell, OB rep3 [OB3_592268]


title: SVZ NSC lineage cell, OB rep3 [OB3_592278]


title: SVZ NSC lineage cell, OB rep3 [OB3_592344]


title: SVZ NSC lineage cell, OB rep3 [OB3_592252]


title: SVZ NSC lineage cell, OB rep3 [OB3_592290]


title: SVZ NSC lineage cell, OB rep3 [OB3_592250]


title: SVZ NSC lineage cell, OB rep3 [OB3_592314]


title: SVZ NSC lineage cell, OB rep3 [OB3_592286]


title: SVZ NSC lineage cell, OB rep3 [OB3_592238]


title: SVZ NSC lineage cell, OB rep3 [OB3_592282]


title: SVZ NSC lineage cell, OB rep3 [OB3_592260]


title: SVZ NSC lineage cell, OB rep3 [OB3_592280]


title: SVZ NSC lineage cell, OB rep3 [OB3_592254]


title: SVZ NSC lineage cell, OB rep3 [OB3_592226]


title: SVZ NSC lineage cell, OB rep3 [OB3_592216]


title: SVZ NSC lineage cell, OB rep3 [OB3_592230]


title: SVZ NSC lineage cell, OB rep3 [OB3_592222]


title: SVZ NSC lineage cell, OB rep3 [OB3_592240]


title: SVZ NSC lineage cell, OB rep3 [OB3_592218]


title: SVZ NSC lineage cell, OB rep3 [OB3_592234]


title: SVZ NSC lineage cell, OB rep3 [OB3_592214]


title: SVZ NSC lineage cell, OB rep3 [OB3_592266]


title: SVZ NSC lineage cell, OB rep3 [OB3_592228]


title: SVZ NSC lineage cell, OB rep3 [OB3_592236]


title: SVZ NSC lineage cell, OB rep3 [OB3_592232]


title: SVZ NSC lineage cell, OB rep3 [OB3_592212]


title: SVZ NSC lineage cell, OB rep3 [OB3_592276]


title: SVZ NSC lineage cell, OB rep3 [OB3_592246]


title: SVZ NSC lineage cell, OB rep3 [OB3_592208]


title: SVZ NSC lineage cell, OB rep3 [OB3_592202]


title: SVZ NSC lineage cell, OB rep3 [OB3_592200]


title: SVZ NSC lineage cell, OB rep3 [OB3_592198]


title: SVZ NSC lineage cell, OB rep3 [OB3_592196]


title: SVZ NSC lineage cell, OB rep3 [OB3_592192]


title: SVZ NSC lineage cell, OB rep3 [OB3_592194]


title: SVZ NSC lineage cell, OB rep3 [OB3_592186]


title: SVZ NSC lineage cell, OB rep3 [OB3_592184]


title: SVZ NSC lineage cell, OB rep3 [OB3_592180]


title: SVZ NSC lineage cell, OB rep3 [OB3_592178]


title: SVZ NSC lineage cell, OB rep3 [OB3_592170]


title: SVZ NSC lineage cell, OB rep3 [OB3_592162]


title: SVZ NSC lineage cell, OB rep3 [OB3_592164]


title: SVZ NSC lineage cell, OB rep3 [OB3_592156]


title: SVZ NSC lineage cell, OB rep3 [OB3_592146]


title: SVZ NSC lineage cell, OB rep3 [OB3_592144]


title: SVZ NSC lineage cell, OB rep3 [OB3_592142]


title: SVZ NSC lineage cell, OB rep3 [OB3_592134]


title: SVZ NSC lineage cell, OB rep3 [OB3_592148]


title: SVZ NSC lineage cell, OB rep3 [OB3_592140]


title: SVZ NSC lineage cell, OB rep3 [OB3_592130]


title: SVZ NSC lineage cell, OB rep3 [OB3_592136]


title: SVZ NSC lineage cell, OB rep3 [OB3_592112]


title: SVZ NSC lineage cell, OB rep3 [OB3_592206]


title: SVZ NSC lineage cell, OB rep3 [OB3_592114]


title: SVZ NSC lineage cell, OB rep3 [OB3_592128]


title: SVZ NSC lineage cell, OB rep3 [OB3_592116]


title: SVZ NSC lineage cell, OB rep3 [OB3_592110]


title: SVZ NSC lineage cell, OB rep3 [OB3_592122]


title: SVZ NSC lineage cell, OB rep3 [OB3_592190]


title: SVZ NSC lineage cell, OB rep3 [OB3_592104]


title: SVZ NSC lineage cell, OB rep3 [OB3_592096]


title: SVZ NSC lineage cell, OB rep3 [OB3_592092]


title: SVZ NSC lineage cell, OB rep3 [OB3_592108]


title: SVZ NSC lineage cell, OB rep3 [OB3_592090]


title: SVZ NSC lineage cell, OB rep3 [OB3_592152]


title: SVZ NSC lineage cell, OB rep3 [OB3_592088]


title: SVZ NSC lineage cell, OB rep3 [OB3_592086]


title: SVZ NSC lineage cell, OB rep3 [OB3_592084]


title: SVZ NSC lineage cell, OB rep2 [OB2_592077]


title: SVZ NSC lineage cell, OB rep2 [OB2_592067]


title: SVZ NSC lineage cell, OB rep2 [OB2_592069]


title: SVZ NSC lineage cell, OB rep2 [OB2_592075]


title: SVZ NSC lineage cell, OB rep2 [OB2_592073]


title: SVZ NSC lineage cell, OB rep2 [OB2_592065]


title: SVZ NSC lineage cell, OB rep2 [OB2_592057]


title: SVZ NSC lineage cell, OB rep2 [OB2_592061]


title: SVZ NSC lineage cell, OB rep2 [OB2_592055]


title: SVZ NSC lineage cell, OB rep2 [OB2_592059]


title: SVZ NSC lineage cell, OB rep2 [OB2_592053]


title: SVZ NSC lineage cell, OB rep2 [OB2_592045]


title: SVZ NSC lineage cell, OB rep2 [OB2_592047]


title: SVZ NSC lineage cell, OB rep2 [OB2_592051]


title: SVZ NSC lineage cell, OB rep2 [OB2_592049]


title: SVZ NSC lineage cell, OB rep2 [OB2_592039]


title: SVZ NSC lineage cell, OB rep2 [OB2_592043]


title: SVZ NSC lineage cell, OB rep3 [OB3_592094]


title: SVZ NSC lineage cell, OB rep3 [OB3_592102]


title: SVZ NSC lineage cell, OB rep2 [OB2_592037]


title: SVZ NSC lineage cell, OB rep2 [OB2_592035]


title: SVZ NSC lineage cell, OB rep2 [OB2_592033]


title: SVZ NSC lineage cell, OB rep2 [OB2_592031]


title: SVZ NSC lineage cell, OB rep2 [OB2_592029]


title: SVZ NSC lineage cell, OB rep2 [OB2_592027]


title: SVZ NSC lineage cell, OB rep2 [OB2_592025]


title: SVZ NSC lineage cell, OB rep2 [OB2_592021]


title: SVZ NSC lineage cell, OB rep2 [OB2_592023]


title: SVZ NSC lineage cell, OB rep2 [OB2_592071]


title: SVZ NSC lineage cell, OB rep2 [OB2_592017]


title: SVZ NSC lineage cell, OB rep2 [OB2_592009]


title: SVZ NSC lineage cell, OB rep2 [OB2_592013]


title: SVZ NSC lineage cell, OB rep2 [OB2_592001]


title: SVZ NSC lineage cell, OB rep2 [OB2_591997]


title: SVZ NSC lineage cell, OB rep2 [OB2_592003]


title: SVZ NSC lineage cell, OB rep2 [OB2_591999]


title: SVZ NSC lineage cell, OB rep2 [OB2_592007]


title: SVZ NSC lineage cell, OB rep2 [OB2_592041]


title: SVZ NSC lineage cell, OB rep2 [OB2_591995]


title: SVZ NSC lineage cell, OB rep2 [OB2_591993]


title: SVZ NSC lineage cell, OB rep2 [OB2_591989]


title: SVZ NSC lineage cell, OB rep2 [OB2_591991]


title: SVZ NSC lineage cell, OB rep2 [OB2_591987]


title: SVZ NSC lineage cell, OB rep2 [OB2_591983]


title: SVZ NSC lineage cell, OB rep2 [OB2_591981]


title: SVZ NSC lineage cell, OB rep2 [OB2_591985]


title: SVZ NSC lineage cell, OB rep2 [OB2_591979]


title: SVZ NSC lineage cell, OB rep2 [OB2_591975]


title: SVZ NSC lineage cell, OB rep2 [OB2_591973]


title: SVZ NSC lineage cell, OB rep2 [OB2_591971]


title: SVZ NSC lineage cell, OB rep2 [OB2_592011]


title: SVZ NSC lineage cell, OB rep2 [OB2_592019]


title: SVZ NSC lineage cell, OB rep2 [OB2_591965]


title: SVZ NSC lineage cell, OB rep2 [OB2_592015]


title: SVZ NSC lineage cell, OB rep2 [OB2_591961]


title: SVZ NSC lineage cell, OB rep2 [OB2_591967]


title: SVZ NSC lineage cell, OB rep2 [OB2_591955]


title: SVZ NSC lineage cell, OB rep2 [OB2_591957]


title: SVZ NSC lineage cell, OB rep2 [OB2_592005]


title: SVZ NSC lineage cell, OB rep2 [OB2_591951]


title: SVZ NSC lineage cell, OB rep2 [OB2_591945]


title: SVZ NSC lineage cell, OB rep2 [OB2_591947]


title: SVZ NSC lineage cell, OB rep2 [OB2_591959]


title: SVZ NSC lineage cell, OB rep2 [OB2_591943]


title: SVZ NSC lineage cell, OB rep2 [OB2_591935]


title: SVZ NSC lineage cell, OB rep2 [OB2_591931]


title: SVZ NSC lineage cell, OB rep2 [OB2_591937]


title: SVZ NSC lineage cell, OB rep2 [OB2_591933]


title: SVZ NSC lineage cell, OB rep2 [OB2_591929]


title: SVZ NSC lineage cell, OB rep2 [OB2_591977]


title: SVZ NSC lineage cell, OB rep2 [OB2_591969]


title: SVZ NSC lineage cell, OB rep2 [OB2_591925]


title: SVZ NSC lineage cell, OB rep2 [OB2_591927]


title: SVZ NSC lineage cell, OB rep2 [OB2_591921]


title: SVZ NSC lineage cell, OB rep2 [OB2_591917]


title: SVZ NSC lineage cell, OB rep2 [OB2_591923]


title: SVZ NSC lineage cell, OB rep2 [OB2_591911]


title: SVZ NSC lineage cell, OB rep2 [OB2_591913]


title: SVZ NSC lineage cell, OB rep2 [OB2_591909]


title: SVZ NSC lineage cell, OB rep2 [OB2_591907]


title: SVZ NSC lineage cell, OB rep2 [OB2_591953]


title: SVZ NSC lineage cell, OB rep2 [OB2_591905]


title: SVZ NSC lineage cell, OB rep2 [OB2_591899]


title: SVZ NSC lineage cell, OB rep2 [OB2_591895]


title: SVZ NSC lineage cell, OB rep2 [OB2_591949]


title: SVZ NSC lineage cell, OB rep2 [OB2_591903]


title: SVZ NSC lineage cell, OB rep2 [OB2_591897]


title: SVZ NSC lineage cell, OB rep2 [OB2_591889]


title: SVZ NSC lineage cell, OB rep2 [OB2_591893]


title: SVZ NSC lineage cell, OB rep2 [OB2_591887]


title: SVZ NSC lineage cell, OB rep2 [OB2_591885]


title: SVZ NSC lineage cell, OB rep2 [OB2_591915]


title: SVZ NSC lineage cell, OB rep2 [OB2_591919]


title: SVZ NSC lineage cell, OB rep2 [OB2_591883]


title: SVZ NSC lineage cell, OB rep2 [OB2_591881]


title: SVZ NSC lineage cell, OB rep2 [OB2_591877]


title: SVZ NSC lineage cell, OB rep2 [OB2_591869]


title: SVZ NSC lineage cell, OB rep2 [OB2_591865]


title: SVZ NSC lineage cell, OB rep2 [OB2_591879]


title: SVZ NSC lineage cell, OB rep2 [OB2_591875]


title: SVZ NSC lineage cell, OB rep2 [OB2_591901]


title: SVZ NSC lineage cell, OB rep2 [OB2_591939]


title: SVZ NSC lineage cell, OB rep2 [OB2_591871]


title: SVZ NSC lineage cell, OB rep2 [OB2_591859]


title: SVZ NSC lineage cell, OB rep2 [OB2_591873]


title: SVZ NSC lineage cell, OB rep2 [OB2_591863]


title: SVZ NSC lineage cell, OB rep2 [OB2_591867]


title: SVZ NSC lineage cell, OB rep2 [OB2_591855]


title: SVZ NSC lineage cell, OB rep2 [OB2_591853]


title: SVZ NSC lineage cell, OB rep2 [OB2_591849]


title: SVZ NSC lineage cell, OB rep2 [OB2_591845]


title: SVZ NSC lineage cell, OB rep2 [OB2_591847]


title: SVZ NSC lineage cell, OB rep2 [OB2_591843]


title: SVZ NSC lineage cell, OB rep2 [OB2_591857]


title: SVZ NSC lineage cell, OB rep2 [OB2_591851]


title: SVZ NSC lineage cell, OB rep2 [OB2_591891]


title: SVZ NSC lineage cell, OB rep2 [OB2_591837]


title: SVZ NSC lineage cell, OB rep2 [OB2_591831]


title: SVZ NSC lineage cell, OB rep2 [OB2_591841]


title: SVZ NSC lineage cell, OB rep2 [OB2_591823]


title: SVZ NSC lineage cell, OB rep2 [OB2_591861]


title: SVZ NSC lineage cell, OB rep2 [OB2_591827]


title: SVZ NSC lineage cell, OB rep2 [OB2_591825]


title: SVZ NSC lineage cell, OB rep2 [OB2_591819]


title: SVZ NSC lineage cell, OB rep2 [OB2_591835]


title: SVZ NSC lineage cell, OB rep2 [OB2_591817]


title: SVZ NSC lineage cell, OB rep2 [OB2_591803]


title: SVZ NSC lineage cell, OB rep2 [OB2_591799]


title: SVZ NSC lineage cell, OB rep2 [OB2_591813]


title: SVZ NSC lineage cell, OB rep2 [OB2_591815]


title: SVZ NSC lineage cell, OB rep2 [OB2_591805]


title: SVZ NSC lineage cell, OB rep2 [OB2_591807]


title: SVZ NSC lineage cell, OB rep2 [OB2_591809]


title: SVZ NSC lineage cell, OB rep2 [OB2_591801]


title: SVZ NSC lineage cell, OB rep2 [OB2_591829]


title: SVZ NSC lineage cell, OB rep2 [OB2_591795]


title: SVZ NSC lineage cell, OB rep2 [OB2_591797]


title: SVZ NSC lineage cell, OB rep2 [OB2_591793]


title: SVZ NSC lineage cell, OB rep2 [OB2_591785]


title: SVZ NSC lineage cell, OB rep2 [OB2_591787]


title: SVZ NSC lineage cell, OB rep2 [OB2_591833]


title: SVZ NSC lineage cell, OB rep2 [OB2_591789]


title: SVZ NSC lineage cell, OB rep2 [OB2_591783]


title: SVZ NSC lineage cell, OB rep2 [OB2_591777]


title: SVZ NSC lineage cell, OB rep2 [OB2_591775]


title: SVZ NSC lineage cell, OB rep2 [OB2_591771]


title: SVZ NSC lineage cell, OB rep2 [OB2_591781]


title: SVZ NSC lineage cell, OB rep2 [OB2_591769]


title: SVZ NSC lineage cell, OB rep2 [OB2_591767]


title: SVZ NSC lineage cell, OB rep2 [OB2_591811]


title: SVZ NSC lineage cell, OB rep2 [OB2_591821]


title: SVZ NSC lineage cell, OB rep2 [OB2_591761]


title: SVZ NSC lineage cell, OB rep2 [OB2_591765]


title: SVZ NSC lineage cell, OB rep2 [OB2_591755]


title: SVZ NSC lineage cell, OB rep2 [OB2_591759]


title: SVZ NSC lineage cell, OB rep2 [OB2_591753]


title: SVZ NSC lineage cell, OB rep2 [OB2_591757]


title: SVZ NSC lineage cell, OB rep2 [OB2_591745]


title: SVZ NSC lineage cell, OB rep2 [OB2_591747]


title: SVZ NSC lineage cell, OB rep2 [OB2_591741]


title: SVZ NSC lineage cell, OB rep2 [OB2_591751]


title: SVZ NSC lineage cell, OB rep2 [OB2_591743]


title: SVZ NSC lineage cell, OB rep2 [OB2_591739]


title: SVZ NSC lineage cell, OB rep2 [OB2_591729]


title: SVZ NSC lineage cell, OB rep2 [OB2_591737]


title: SVZ NSC lineage cell, OB rep2 [OB2_591733]


title: SVZ NSC lineage cell, OB rep2 [OB2_591727]


title: SVZ NSC lineage cell, OB rep2 [OB2_591731]


title: SVZ NSC lineage cell, OB rep2 [OB2_591779]


title: SVZ NSC lineage cell, OB rep2 [OB2_591725]


title: SVZ NSC lineage cell, OB rep2 [OB2_591723]


title: SVZ NSC lineage cell, OB rep2 [OB2_591719]


title: SVZ NSC lineage cell, OB rep2 [OB2_591717]


title: SVZ NSC lineage cell, OB rep2 [OB2_591721]


title: SVZ NSC lineage cell, OB rep2 [OB2_591763]


title: SVZ NSC lineage cell, OB rep2 [OB2_591711]


title: SVZ NSC lineage cell, OB rep2 [OB2_591715]


title: SVZ NSC lineage cell, OB rep2 [OB2_591713]


title: SVZ NSC lineage cell, OB rep2 [OB2_591709]


title: SVZ NSC lineage cell, OB rep2 [OB2_591705]


title: SVZ NSC lineage cell, OB rep2 [OB2_591703]


title: SVZ NSC lineage cell, OB rep2 [OB2_591701]


title: SVZ NSC lineage cell, OB rep2 [OB2_591707]


title: SVZ NSC lineage cell, OB rep2 [OB2_591695]


title: SVZ NSC lineage cell, OB rep2 [OB2_591691]


title: SVZ NSC lineage cell, OB rep2 [OB2_591693]


title: SVZ NSC lineage cell, OB rep2 [OB2_591699]


title: SVZ NSC lineage cell, OB rep2 [OB2_591687]


title: SVZ NSC lineage cell, OB rep2 [OB2_591689]


title: SVZ NSC lineage cell, OB rep2 [OB2_591735]


title: SVZ NSC lineage cell, OB rep2 [OB2_591679]


title: SVZ NSC lineage cell, OB rep2 [OB2_591683]


title: SVZ NSC lineage cell, OB rep2 [OB2_591677]


title: SVZ NSC lineage cell, OB rep2 [OB2_591671]


title: SVZ NSC lineage cell, OB rep2 [OB2_591669]


title: SVZ NSC lineage cell, OB rep2 [OB2_591659]


title: SVZ NSC lineage cell, OB rep2 [OB2_591661]


title: SVZ NSC lineage cell, OB rep2 [OB2_591675]


title: SVZ NSC lineage cell, OB rep2 [OB2_591673]


title: SVZ NSC lineage cell, OB rep2 [OB2_591663]


title: SVZ NSC lineage cell, OB rep2 [OB2_591657]


title: SVZ NSC lineage cell, OB rep2 [OB2_591667]


title: SVZ NSC lineage cell, OB rep2 [OB2_591655]


title: SVZ NSC lineage cell, OB rep2 [OB2_591685]


title: SVZ NSC lineage cell, OB rep2 [OB2_591653]


title: SVZ NSC lineage cell, OB rep2 [OB2_591647]


title: SVZ NSC lineage cell, OB rep2 [OB2_591697]


title: SVZ NSC lineage cell, OB rep2 [OB2_591645]


title: SVZ NSC lineage cell, OB rep2 [OB2_591651]


title: SVZ NSC lineage cell, OB rep2 [OB2_591649]


title: SVZ NSC lineage cell, OB rep2 [OB2_591635]


title: SVZ NSC lineage cell, OB rep2 [OB2_591637]


title: SVZ NSC lineage cell, OB rep2 [OB2_591641]


title: SVZ NSC lineage cell, OB rep2 [OB2_591629]


title: SVZ NSC lineage cell, OB rep2 [OB2_591631]


title: SVZ NSC lineage cell, OB rep2 [OB2_591681]


title: SVZ NSC lineage cell, OB rep2 [OB2_591627]


title: SVZ NSC lineage cell, OB rep2 [OB2_591625]


title: SVZ NSC lineage cell, OB rep2 [OB2_591639]


title: SVZ NSC lineage cell, OB rep2 [OB2_591633]


title: SVZ NSC lineage cell, OB rep2 [OB2_591619]


title: SVZ NSC lineage cell, OB rep2 [OB2_591607]


title: SVZ NSC lineage cell, OB rep2 [OB2_591615]


title: SVZ NSC lineage cell, OB rep2 [OB2_591617]


title: SVZ NSC lineage cell, OB rep2 [OB2_591611]


title: SVZ NSC lineage cell, OB rep2 [OB2_591621]


title: SVZ NSC lineage cell, OB rep2 [OB2_591665]


title: SVZ NSC lineage cell, OB rep2 [OB2_591605]


title: SVZ NSC lineage cell, OB rep2 [OB2_591613]


title: SVZ NSC lineage cell, OB rep2 [OB2_591609]


title: SVZ NSC lineage cell, OB rep2 [OB2_591601]


title: SVZ NSC lineage cell, OB rep2 [OB2_591603]


title: SVZ NSC lineage cell, OB rep2 [OB2_591749]


title: SVZ NSC lineage cell, OB rep2 [OB2_591599]


title: SVZ NSC lineage cell, OB rep2 [OB2_591589]


title: SVZ NSC lineage cell, OB rep2 [OB2_591587]


title: SVZ NSC lineage cell, OB rep2 [OB2_591593]


title: SVZ NSC lineage cell, OB rep2 [OB2_591595]


title: SVZ NSC lineage cell, OB rep2 [OB2_591579]


title: SVZ NSC lineage cell, OB rep2 [OB2_591577]


title: SVZ NSC lineage cell, OB rep2 [OB2_591569]


title: SVZ NSC lineage cell, OB rep2 [OB2_591585]


title: SVZ NSC lineage cell, OB rep2 [OB2_591623]


title: SVZ NSC lineage cell, OB rep2 [OB2_591581]


title: SVZ NSC lineage cell, OB rep2 [OB2_591571]


title: SVZ NSC lineage cell, OB rep2 [OB2_591567]


title: SVZ NSC lineage cell, OB rep2 [OB2_591573]


title: SVZ NSC lineage cell, OB rep2 [OB2_591575]


title: SVZ NSC lineage cell, OB rep2 [OB2_591565]


title: SVZ NSC lineage cell, OB rep2 [OB2_591563]


title: SVZ NSC lineage cell, OB rep2 [OB2_591559]


title: SVZ NSC lineage cell, OB rep2 [OB2_591557]


title: SVZ NSC lineage cell, OB rep2 [OB2_591553]


title: SVZ NSC lineage cell, OB rep2 [OB2_591555]


title: SVZ NSC lineage cell, OB rep2 [OB2_591591]


title: SVZ NSC lineage cell, OB rep2 [OB2_591551]


title: SVZ NSC lineage cell, OB rep2 [OB2_591547]


title: SVZ NSC lineage cell, OB rep2 [OB2_591545]


title: SVZ NSC lineage cell, OB rep2 [OB2_591597]


title: SVZ NSC lineage cell, OB rep2 [OB2_591541]


title: SVZ NSC lineage cell, OB rep2 [OB2_591537]


title: SVZ NSC lineage cell, OB rep2 [OB2_591533]


title: SVZ NSC lineage cell, OB rep2 [OB2_591539]


title: SVZ NSC lineage cell, OB rep2 [OB2_591531]


title: SVZ NSC lineage cell, OB rep2 [OB2_591529]


title: SVZ NSC lineage cell, OB rep2 [OB2_591583]


title: SVZ NSC lineage cell, OB rep2 [OB2_591535]


title: SVZ NSC lineage cell, OB rep2 [OB2_591527]


title: SVZ NSC lineage cell, OB rep2 [OB2_591525]


title: SVZ NSC lineage cell, OB rep2 [OB2_591523]


title: SVZ NSC lineage cell, OB rep2 [OB2_591521]


title: SVZ NSC lineage cell, OB rep2 [OB2_591515]


title: SVZ NSC lineage cell, OB rep2 [OB2_591509]


title: SVZ NSC lineage cell, OB rep2 [OB2_591519]


title: SVZ NSC lineage cell, OB rep2 [OB2_591513]


title: SVZ NSC lineage cell, OB rep2 [OB2_591517]


title: SVZ NSC lineage cell, OB rep2 [OB2_591503]


title: SVZ NSC lineage cell, OB rep2 [OB2_591501]


title: SVZ NSC lineage cell, OB rep2 [OB2_591499]


title: SVZ NSC lineage cell, OB rep2 [OB2_591505]


title: SVZ NSC lineage cell, OB rep2 [OB2_591495]


title: SVZ NSC lineage cell, OB rep2 [OB2_591485]


title: SVZ NSC lineage cell, OB rep2 [OB2_591489]


title: SVZ NSC lineage cell, OB rep2 [OB2_591481]


title: SVZ NSC lineage cell, OB rep2 [OB2_591493]


title: SVZ NSC lineage cell, OB rep2 [OB2_591479]


title: SVZ NSC lineage cell, OB rep2 [OB2_591477]


title: SVZ NSC lineage cell, OB rep2 [OB2_591473]


title: SVZ NSC lineage cell, OB rep2 [OB2_591467]


title: SVZ NSC lineage cell, OB rep2 [OB2_591507]


title: SVZ NSC lineage cell, OB rep2 [OB2_591465]


title: SVZ NSC lineage cell, OB rep2 [OB2_591471]


title: SVZ NSC lineage cell, OB rep2 [OB2_591461]


title: SVZ NSC lineage cell, OB rep2 [OB2_591455]


title: SVZ NSC lineage cell, OB rep2 [OB2_591459]


title: SVZ NSC lineage cell, OB rep2 [OB2_591511]


title: SVZ NSC lineage cell, OB rep2 [OB2_591457]


title: SVZ NSC lineage cell, OB rep2 [OB2_591449]


title: SVZ NSC lineage cell, OB rep2 [OB2_591445]


title: SVZ NSC lineage cell, OB rep2 [OB2_591453]


title: SVZ NSC lineage cell, OB rep2 [OB2_591483]


title: SVZ NSC lineage cell, OB rep2 [OB2_591549]


title: SVZ NSC lineage cell, OB rep2 [OB2_591437]


title: SVZ NSC lineage cell, OB rep2 [OB2_591439]


title: SVZ NSC lineage cell, OB rep2 [OB2_591475]


title: SVZ NSC lineage cell, OB rep2 [OB2_591435]


title: SVZ NSC lineage cell, OB rep2 [OB2_591433]


title: SVZ NSC lineage cell, OB rep2 [OB2_591443]


title: SVZ NSC lineage cell, OB rep2 [OB2_591441]


title: SVZ NSC lineage cell, OB rep2 [OB2_591427]


title: SVZ NSC lineage cell, OB rep2 [OB2_591425]


title: SVZ NSC lineage cell, OB rep2 [OB2_591431]


title: SVZ NSC lineage cell, OB rep2 [OB2_591423]


title: SVZ NSC lineage cell, OB rep2 [OB2_591463]


title: SVZ NSC lineage cell, OB rep2 [OB2_591417]


title: SVZ NSC lineage cell, OB rep2 [OB2_591415]


title: SVZ NSC lineage cell, OB rep2 [OB2_591411]


title: SVZ NSC lineage cell, OB rep2 [OB2_591429]


title: SVZ NSC lineage cell, OB rep2 [OB2_591407]


title: SVZ NSC lineage cell, OB rep2 [OB2_591405]


title: SVZ NSC lineage cell, OB rep2 [OB2_591409]


title: SVZ NSC lineage cell, OB rep2 [OB2_591401]


title: SVZ NSC lineage cell, OB rep2 [OB2_591399]


title: SVZ NSC lineage cell, OB rep2 [OB2_591395]


title: SVZ NSC lineage cell, OB rep2 [OB2_591397]


title: SVZ NSC lineage cell, OB rep2 [OB2_591393]


title: SVZ NSC lineage cell, OB rep2 [OB2_591387]


title: SVZ NSC lineage cell, OB rep2 [OB2_591385]


title: SVZ NSC lineage cell, OB rep2 [OB2_591383]


title: SVZ NSC lineage cell, OB rep2 [OB2_591377]


title: SVZ NSC lineage cell, OB rep2 [OB2_591375]


title: SVZ NSC lineage cell, OB rep2 [OB2_591379]


title: SVZ NSC lineage cell, OB rep2 [OB2_591373]


title: SVZ NSC lineage cell, OB rep2 [OB2_591367]


title: SVZ NSC lineage cell, OB rep2 [OB2_591369]


title: SVZ NSC lineage cell, OB rep2 [OB2_591371]


title: SVZ NSC lineage cell, OB rep2 [OB2_591365]


title: SVZ NSC lineage cell, OB rep2 [OB2_591361]


title: SVZ NSC lineage cell, OB rep2 [OB2_591357]


title: SVZ NSC lineage cell, OB rep2 [OB2_591403]


title: SVZ NSC lineage cell, OB rep2 [OB2_591353]


title: SVZ NSC lineage cell, OB rep2 [OB2_591351]


title: SVZ NSC lineage cell, OB rep2 [OB2_591355]


title: SVZ NSC lineage cell, OB rep2 [OB2_591391]


title: SVZ NSC lineage cell, OB rep2 [OB2_591349]


title: SVZ NSC lineage cell, OB rep2 [OB2_591389]


title: SVZ NSC lineage cell, OB rep2 [OB2_591347]


title: SVZ NSC lineage cell, OB rep2 [OB2_591491]


title: SVZ NSC lineage cell, OB rep2 [OB2_591341]


title: SVZ NSC lineage cell, OB rep2 [OB2_591345]


title: SVZ NSC lineage cell, OB rep2 [OB2_591343]


title: SVZ NSC lineage cell, OB rep2 [OB2_591337]


title: SVZ NSC lineage cell, OB rep2 [OB2_591339]


title: SVZ NSC lineage cell, OB rep2 [OB2_591335]


title: SVZ NSC lineage cell, OB rep2 [OB2_591331]


title: SVZ NSC lineage cell, OB rep2 [OB2_591317]


title: SVZ NSC lineage cell, OB rep2 [OB2_591325]


title: SVZ NSC lineage cell, OB rep2 [OB2_591313]


title: SVZ NSC lineage cell, OB rep2 [OB2_591329]


title: SVZ NSC lineage cell, OB rep2 [OB2_591359]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_591264]

source_name: Brain, SVZ

value: Brain, SVZ


title: SVZ NSC lineage cell, OB rep2 [OB2_591315]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_591240]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_591148]


title: SVZ NSC lineage cell, OB rep2 [OB2_591321]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_591206]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_591194]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_591124]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_591236]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_591070]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_591104]


title: SVZ NSC lineage cell, OB rep2 [OB2_591333]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_591044]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_591208]


title: SVZ NSC lineage cell, OB rep2 [OB2_591323]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590972]


title: SVZ NSC lineage cell, OB rep2 [OB2_591327]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590964]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_591026]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590962]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_591030]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590992]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590956]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590948]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590984]


title: SVZ NSC lineage cell, OB rep2 [OB2_591487]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590938]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590934]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590932]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590974]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590966]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590968]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590924]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_591036]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590970]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590950]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590958]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590916]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590912]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590914]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590910]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590906]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590944]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590926]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590928]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590898]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590922]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590920]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590890]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590954]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590888]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590886]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590884]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590908]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590880]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590940]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590878]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590902]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590870]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590900]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590892]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590876]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590874]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590904]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590872]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590862]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590860]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590856]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590858]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590866]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590850]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590846]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590844]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590864]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590848]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590896]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590854]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590836]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590868]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590852]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590834]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590882]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590842]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590840]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590828]


title: SVZ NSC lineage cell, OB rep2 [OB2_591451]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590838]


title: SVZ NSC lineage cell, OB rep2 [OB2_591469]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590832]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590830]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590824]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590820]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590822]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590818]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590814]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590812]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590808]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590816]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590810]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590802]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590800]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590804]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590798]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590794]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590796]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590792]


title: SVZ NSC lineage cell, SVZ rep2 [SVZ2_590788]