title: CUT_and_Tag_S7A_H2AZ_dTAG_4h_rep2

city: Beijing

first: Junyi

last: Zhu

address: Peking University, No.5 YiHeYuan Road, Haidian District

department: School of Life sciences

institute: Peking University

description:
Library name: sample169
CUT_and_Tag_H2AZ_S7A_Rawcounts.csv
H2AZ_S7A_DEseq2_results.csv

data_processing:
ChIP-seq and CUT&Tag raw data were processed using a custom pipeline that builds on published workflows with minor modifications.  Briefly, reads were firstly trimmed for adapter sequences with trim_galore (v.0.6.2).
Then trimmed reads were mapped to human reference genome (hg38) (Endogeneous RNAPII CTD antibody, H3K36me3, H2A.ZAc and H2A.Z) or the concatenated index of human reference genome (hg38) and  mouse reference genome (mm10) (Mutant ChIP-seq) using Bowtie2 (v.2.2.3).
For endogeneous RNAPII CTD antibody ChIP-seq, the final bam files were converted to bigwig file using bamCoverage with RPGC normalize method. For mutant ChIP-seq, bam file were converted to bigwig with normalization of mm10 reads. For H3K36me3, gene body reads were counted with featureCounts (v.2.0.1) and TSS reads were counted for H2A.Zac and H2A.Z.
Differential expression genes were identified with R package DESeq2 (v.1.24.0) with default settings.
Assembly: hg38 or Concatenated index of hg38 and mm10
Supplementary files format and content: bigwig
Supplementary files format and content: Comma-delimited text file includes raw counts and analysis results from deseq2
Library strategy: CUT&Tag

growth_protocol: A549 cells were cultured in basic Dulbecco's modified Eagle's medium (DMEM)-high glucose (SIGMA#D5796-500mL), including 1× penicillin/streptomycin (Gibco#15140-122) and 10% fetal bovine serum (FBS) (VivaCell#C04001-500). The cells were incubated at 37 °C with 5% CO2 in a humidified incubator. The Schneider 2(S2) cells were cultured in complete Schneider’s Drosophila Medium (Invitrogen, Cat#21720024) supplemented with 10% FBS (CellMax, Cat#SA311.02) at room temperature.

extract_protocol:
Sample preparation were similar as standard ChIP-seq protocols with some modifications. Briefly, cells were fixed with 1% formalaldehyde, and chromatin fractions were seperated. After sonication, free chromatin fractions were incubated with corresponding antibodies overnight at 4°C with rotations. Antibody-bound proteins were captured with potein G beads, and after washing, protein-bound genomic DNA was extracted and purified. For mutant cells, HA-beads were incubated overnight at 4°C with rotations. For, H2AZ CUT&Tag experiments, samples are collected and performed CUT&Tag assay with Hyperactive Universal CUT&Tag Assay Kit for Illumina (Vazyme, Cat#TD903) following the manufacturers' instruction. Briefly, 0.1% formalaldehyde crosslinked cells were permealization, and incubated with corresponding antibodies overnight at 4℃. After secondary antibody conjugation, samples were mixed with protein A/G-Tnp, and cut DNAs were purified.
Library construction was performed following the VAHTS Universal Pro DNA Library Prep Kit for Illumina (Vazyme #ND608) instructions. For CUT&Tag experiments, library construction was performed following the VAHTS with Hyperactive Universal CUT&Tag Assay Kit for Illumina (Vazyme, Cat#TD903) instructions.

treatment_protocol: Individual cell line were  pretreated with or without 2 μg/ml Doxycycline for 24 h to induce mutant RPB1 expression, then treated with or without 100 nM dTAGV-1 for 4h to induce endogeneous RPB1 degradation. For spike-in, 10% mESCs cells were used. For endogeneous RNAPII CTD antibody ChIP, WT A549 cells were used.

value: RPB1 degron, S7A RPB1 complement

value: Dox (2 μg/mL) for 24 hours followd with dTAGV-1 (100 nM) for 4 hours

tag: chip antibody

value: H2A.Z antibody (Abcam #ab4174)


title: BE Group,input,1

status: Public on Oct 28 2025

type: SRA

city: Jinan

first: Ke

last: Zou

address: No. 105, Jiefang Road, Jinan, Shandong 250013, China

country: China

institute: Shandong university

library_source: transcriptomic

data_processing:
Libraries were qualified using BioAnalyzer 2100 system (Agilent Technologies, Inc., United States) bioanalyzer and then sequenced in a NovaSeq platform (Illumina) with 150 bp paired-end reads.
Paired-end reads were harvested from Illumina NovaSeq 6000 sequencer, and were quality controlled by Q30.
After 3’ adaptor-trimming and low quality reads removing by cutadapt software (v1.9.3).
Clean reads of all libraries were aligned to the reference genome (RN5)  by Hisat2 software (v2.0.4).
Methylated sites on RNAs (peaks) were identified by MACS software.
Differentially methylated sites were identified by diffReps.
These peaks identified by both softwares overlapping with exons of mRNA were figured out and chosen by home-made scripts.
Assembly: RN5
Supplementary files format and content: xlsx for each sample

molecule: total RNA

organism: Rattus norvegicus

source_name: esophageal tissue

extract_protocol:
Total RNA was subjected to immunoprecipitation with the GenSeq® m6A-IP Kit (GenSeq Inc.) followed the manufacturer’s instructions.
RNA libraries for IP and input samples were then constructed with GenSeq® Low Input Whole RNA Library Prep Kit (GenSeq, Inc.) by following the manufacturer’s instructions.

tag: tissue

value: esophageal tissue

tag: antibody

tag: treatment

value: esophagoduodenostomy(EDA) surgery


title: CUT_and_Tag_S7A_H2AZ_dTAG_4h_rep1

description:
Library name: sample168
CUT_and_Tag_H2AZ_S7A_Rawcounts.csv
H2AZ_S7A_DEseq2_results.csv

library_source: genomic

molecule: genomic DNA

organism: Homo sapiens

tag: cell line

tag: genotype


title: BUC5254A8

city: London

first: Probir

last: Chakravarty

address: 1 Midland Road

country: United Kingdom

institute: Francis Crick Institute

description: Library name: BUC5254A8

data_processing:
The RSEM package (version 1.3.0) (Li and Dewey, 2011) was used in conjunction with the STAR alignment algorithm (version 2.5.2a) (Dobin et al., 2013) for the mapping and subsequent gene-level counting of the sequenced reads with respect to Ensembl mouse GRCm38 release 89  transcriptome
Normalisation of raw count data and differential expression analysis was performed with the DESeq2 package (version 1.16.1)  within the R programming environment (version 3.3.1)
Assembly: GRCm38 release 89
Supplementary files format and content: VST log2 normalised expression values. Rows = genes; Columns = samples.
Supplementary files format and content: raw_counts. Rows = genes; Columns = samples.

molecule: polyA RNA

organism: Mus musculus

source_name: Dendritic Cells

growth_protocol: RPMI 1640 media with 10% FCS, PSG, NaPyruvate, NEAA, HEPES, 2-Me

extract_protocol:
Qiagen Rneasy Kit
NEBNext Ultra II Directional PolyA mRNA Kit

treatment_protocol: DNGR-1 ligand (F-actin plus myosin II)

tag: cell type

value: Dendritic Cells

value: Unstimulated


title: CUT_and_Tag_S7A_H2AZ_dTAG_0h_rep2

description:
Library name: sample167
CUT_and_Tag_H2AZ_S7A_Rawcounts.csv
H2AZ_S7A_DEseq2_results.csv

value: Untreated


title: CUT_and_Tag_S5A_H2AZ_dTAG_4h_rep2

description:
Library name: sample165
CUT_and_Tag_H2AZ_S5A_Rawcounts.csv
H2AZ_S5A_DEseq2_results.csv

value: RPB1 degron, S5A RPB1 complement


title: CUT_and_Tag_S7A_H2AZ_dTAG_0h_rep1

description:
Library name: sample166
CUT_and_Tag_H2AZ_S7A_Rawcounts.csv
H2AZ_S7A_DEseq2_results.csv


title: CUT_and_Tag_S5A_H2AZ_dTAG_0h_rep1

description:
Library name: sample162
CUT_and_Tag_H2AZ_S5A_Rawcounts.csv
H2AZ_S5A_DEseq2_results.csv


title: CUT_and_Tag_T4V_H2AZ_dTAG_4h_rep2

description:
Library name: sample161
CUT_and_Tag_H2AZ_T4V_Rawcounts.csv
H2AZ_T4V_DEseq2_results.csv

value: RPB1 degron, T4V RPB1 complement


title: BUC5254A7

description: Library name: BUC5254A7


title: CUT_and_Tag_T4V_H2AZ_dTAG_4h_rep1

description:
Library name: sample160
CUT_and_Tag_H2AZ_T4V_Rawcounts.csv
H2AZ_T4V_DEseq2_results.csv


title: CUT_and_Tag_T4V_H2AZ_dTAG_0h_rep2

description:
Library name: sample159
CUT_and_Tag_H2AZ_T4V_Rawcounts.csv
H2AZ_T4V_DEseq2_results.csv


title: NC Group,IP,2

value: no treatment


title: CUT_and_Tag_S2A_H2AZ_dTAG_0h_rep2

description:
Library name: sample155
CUT_and_Tag_H2AZ_S2A_Rawcounts.csv
H2AZ_S2A_DEseq2_results.csv

value: RPB1 degron, S2A RPB1 complement


title: BUC5254A1

description: Library name: BUC5254A1

value: WT


title: CUT_and_Tag_S5A_H2AZ_dTAG_0h_rep2

description:
Library name: sample163
CUT_and_Tag_H2AZ_S5A_Rawcounts.csv
H2AZ_S5A_DEseq2_results.csv


title: CUT_and_Tag_S2A_H2AZ_dTAG_0h_rep1

description:
Library name: sample154
CUT_and_Tag_H2AZ_S2A_Rawcounts.csv
H2AZ_S2A_DEseq2_results.csv


title: CUT_and_Tag_Y1F_H2AZ_dTAG_4h_rep1

description:
Library name: sample152
CUT_and_Tag_H2AZ_Y1F_Rawcounts.csv
H2AZ_Y1F_DEseq2_results.csv

value: RPB1 degron, Y1F RPB1 complement


title: NC Group,IP,1


title: CUT_and_Tag_WT_H2AZ_dTAG_4h_rep2

description:
Library name: sample149
CUT_and_Tag_H2AZ_WT_Rawcounts.csv
H2AZ_WT_DEseq2_results.csv

value: RPB1 degron, WT RPB1 complement


title: CUT_and_Tag_WT_H2AZ_dTAG_4h_rep1

description:
Library name: sample148
CUT_and_Tag_H2AZ_WT_Rawcounts.csv
H2AZ_WT_DEseq2_results.csv


title: CUT_and_Tag_WT_H2AZ_dTAG_0h_rep1

description:
Library name: sample146
CUT_and_Tag_H2AZ_WT_Rawcounts.csv
H2AZ_WT_DEseq2_results.csv


title: CUT_and_Tag_Degron_RPB1_H2AZ_dTAG_4h_rep2

description:
Library name: sample145
CUT_and_Tag_H2AZ_Degron_RPB1_Rawcounts.csv
H2AZ_Degron_RPB1_DEseq2_results.csv

value: RPB1 degron

value: dTAGV-1 (100 nM) for 4 hours


title: CUT_and_Tag_S5A_H2AZ_dTAG_4h_rep1

description:
Library name: sample164
CUT_and_Tag_H2AZ_S5A_Rawcounts.csv
H2AZ_S5A_DEseq2_results.csv


title: CUT_and_Tag_Degron_RPB1_H2AZ_dTAG_0h_rep1

description:
Library name: sample142
CUT_and_Tag_H2AZ_Degron_RPB1_Rawcounts.csv
H2AZ_Degron_RPB1_DEseq2_results.csv


title: CUT_and_Tag_WT_H2AZ_dTAG_0h_rep2

description:
Library name: sample147
CUT_and_Tag_H2AZ_WT_Rawcounts.csv
H2AZ_WT_DEseq2_results.csv


title: CUT_and_Tag_Degron_RPB1_H2AZ_dTAG_0h_rep2

description:
Library name: sample143
CUT_and_Tag_H2AZ_Degron_RPB1_Rawcounts.csv
H2AZ_Degron_RPB1_DEseq2_results.csv


title: BatchE_ethanol extract of Sayeok-tang (Low dose)_second_sequencing_rep3

city: Daejeon

first: Musun

last: Park

address: 1672 Yuseong-daero, Yuseong-gu

country: South Korea

institute: Korean Institute of Oriental Medicine

description: sample 15-3-6

data_processing:
Quality of RNA-seq raw data was assessed using FastQC (v0.11.9), and the common regions of MGISEQ adapter sequences were removed using TrimGalore (v0.6.5). The trimmed reads were then aligned to the human reference genome hg38 using the STAR (v2.7.3a) with default settings. Transcript abundance per gene including expected read counts and transcripts per million (TPM) were quantified using RSEM (v1.3.3) with gene annotation GRCh38.84.
Assembly: GRCh38.84
Supplementary files format and content: tab-delimited text files include Expected counts for each Sample

growth_protocol: Human astrocytoma cell line, SW1783 (HTB-13) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). SW1783 cells were maintained in Leibovitz's L-15 medium supplemented with 10% (v/v) heat-inactivated FBS, 100 IU/mL penicillin, and 100 mg/mL streptomycin at 37 °C, 0% CO2 incubator. The cell was subcultured every 3 or 4 days, depending on the cell density.

extract_protocol:
Total RNA was isolated according to the manufacturer’s protocol. The concentration of the isolated RNA was determined using an Agilent RNA 6000 Nano Kit (Agilent Technologies, Waldbronn, Germany), and RNA quality was evaluated by assessing the RNA integrity number (RIN > 7).
A total RNA (1 microgr) was processed to prepare a mRNA sequencing library using the MGIEasy RNA Directional Library Prep kit (#1000006386, MGI Tech Co., Ltd., China) according to the manufacturer’s instruction. The library was quantified using the QauntiFluor® ssDNA System (E3190, Promega Corporation, WI, USA). The prepared DNA nanoball was sequenced on the MGIseq system (MGI Tech Co., Ltd., China) with 100 bp paired-end reads.

treatment_protocol: Leibovitz's L-15, phosphate-buffered saline (PBS), TrypLE Express, penicillin-streptomycin, and fetal bovine serum (FBS) were purchased from Gibco (Grand Island, NY, USA). Cell culture flasks and multiwell culture plates were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Dimethyl sulfoxide (DMSO) and QIAzol lysis reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA) and Qiagen (Germantown, MD, USA), respectively. The dried medicinal plants, prepared in compliance with the Korean Pharmacopoeia, were supplied from Kwangmyung-dang Medicinal Herbs Co. (Ulsan, Republic of Korea). The samples used in the experiment underwent organoleptic examination by Dr. Choi Goya, an herbal medicine organoleptic examination expert appointed by the Korea Food and Drug Administration, and were additionally identified at the species level by analyzing the DNA barcode region sequences. Voucher specimen were deposited in the Korean Herbarium of Standard Herbal Resources, Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine, Naju, Republic of Korea . All herbs and extracts can be obtained from the Oriental Medicine Resources Research Center (KIOM, https://oasis.kiom.re.kr/herblib). A hot water and 70% ethanol extract of each plant was prepared and supplied by KOC Biotech Co. (Daejeon, Republic of Korea). Briefly, dried plants (1,000 g) were pulverized and extracted in hot distilled water (10 L) for 3 h under a reflux extraction system (MS-DM609; MTOPS, Seoul, Republic of Korea) or in 70% ethanol (15L) for 1 h under ultrasonication system (VCP-20, Lab companion, Dajeon, Republic of Korea) twice. The extract solution was filtered through a 5 µm cartridge filter, concentrated in a rotary evaporator (Ev-1020, SciLab, Seoul, Republic of Korea), and then lyophilized in a freeze dryer (LP-20, Ilshin-Bio-Base, Dongduchen, Republic of Korea) to obtain the final extract. The extract was finely homogenized and packed in a glass bottle containing a desiccant silica gel. The decoction was prepared by mixing each extract, considering the composition ratio and extract yield of a single medicinal herb, and homogenized, according to the Korean Pharmacopoeia. For the in vitro treatment, extracts (100 mg) were vigorously vortexed for 30 min in 10 mL phosphate-buffered saline (PBS; Thermo Fisher Scientific, Rockford, IL, USA) containing 2% DMSO and then sterilized by filtering through a 0.22 µm membrane to obtain a stock solution (10 mg/mL); the stock solution was aliquoted in small volumes and stored at -80°C until use.

value: Sayeok-tang ethanol extract 20 microg/mL

tag: time


title: BatchE_ethanol extract of Sayeok-tang (Medium dose)_second_sequencing_rep3

description: sample 15-3-5

value: Sayeok-tang ethanol extract 100 microg/mL


title: BatchE_ethanol extract of Sayeok-tang (High dose)_second_sequencing_rep3

description: sample 15-3-4

value: Sayeok-tang ethanol extract 500 microg/mL


title: BatchE_ethanol extract of Bojungikgi-tang (Medium dose)_second_sequencing_rep3

description: sample 15-3-2

value: Bojungikgi-tang ethanol extract 100 microg/mL


title: BatchE_ethanol extract of Bojungikgi-tang (High dose)_second_sequencing_rep3

description: sample 15-3-1

value: Bojungikgi-tang ethanol extract 500 microg/mL


title: CUT_and_Tag_T4V_H2AZ_dTAG_0h_rep1

description:
Library name: sample158
CUT_and_Tag_H2AZ_T4V_Rawcounts.csv
H2AZ_T4V_DEseq2_results.csv

value: H2A.Z antibody (Abcam #ab4174)


title: BatchE_ethanol extract of Bojungikgi-tang (Low dose)_second_sequencing_rep3

description: sample 15-3-3

value: Bojungikgi-tang ethanol extract 20 microg/mL


title: CUT_and_Tag_S2A_H2AZ_dTAG_4h_rep2

description:
Library name: sample157
CUT_and_Tag_H2AZ_S2A_Rawcounts.csv
H2AZ_S2A_DEseq2_results.csv

value: Dox (2 μg/mL) for 24 hours followd with dTAGV-1 (100 nM) for 4 hours


title: BatchE_ethanol extract of Sayeok-tang (Medium dose)_second_sequencing_rep2

description: sample 15-2-5


title: BatchE_ethanol extract of Sayeok-tang (Low dose)_second_sequencing_rep2

description: sample 15-2-6


title: CUT_and_Tag_S2A_H2AZ_dTAG_4h_rep1

description:
Library name: sample156
CUT_and_Tag_H2AZ_S2A_Rawcounts.csv
H2AZ_S2A_DEseq2_results.csv


title: CUT_and_Tag_Y1F_H2AZ_dTAG_4h_rep2

description:
Library name: sample153
CUT_and_Tag_H2AZ_Y1F_Rawcounts.csv
H2AZ_Y1F_DEseq2_results.csv


title: BatchE_ethanol extract of Bojungikgi-tang (Medium dose)_second_sequencing_rep2

description: sample 15-2-2


title: BatchE_ethanol extract of Sayeok-tang (Low dose)_second_sequencing_rep1

description: sample 15-1-6


title: CUT_and_Tag_Y1F_H2AZ_dTAG_0h_rep2

description:
Library name: sample151
CUT_and_Tag_H2AZ_Y1F_Rawcounts.csv
H2AZ_Y1F_DEseq2_results.csv

value: Untreated


title: BatchE_ethanol extract of Sayeok-tang (High dose)_second_sequencing_rep2

description: sample 15-2-4


title: BatchE_ethanol extract of Sayeok-tang (Medium dose)_second_sequencing_rep1

description: sample 15-1-5


title: BatchE_ethanol extract of Bojungikgi-tang (High dose)_second_sequencing_rep2

description: sample 15-2-1


title: BatchE_ethanol extract of Bojungikgi-tang (Low dose)_second_sequencing_rep2

description: sample 15-2-3


title: BatchE_ethanol extract of Bojungikgi-tang (Low dose)_second_sequencing_rep1

description: sample 15-1-3


title: BatchE_ethanol extract of Sayeok-tang (High dose)_second_sequencing_rep1

description: sample 15-1-4


title: CUT_and_Tag_Degron_RPB1_H2AZ_dTAG_4h_rep1

description:
Library name: sample144
CUT_and_Tag_H2AZ_Degron_RPB1_Rawcounts.csv
H2AZ_Degron_RPB1_DEseq2_results.csv


title: BatchE_ethanol extract of Bojungikgi-tang (Medium dose)_second_sequencing_rep1

description: sample 15-1-2


title: BatchE_ethanol extract of Bojungikgi-tang (High dose)_second_sequencing_rep1

description: sample 15-1-1


title: BatchE_ethanol extract of Banhasasim-tang (Medium dose)_second_sequencing_rep3

description: sample 14-3-5

value: Banhasasim-tang ethanol extract 100 microg/mL


title: BatchE_ethanol extract of Banhasasim-tang (Low dose)_second_sequencing_rep3

description: sample 14-3-6

value: Banhasasim-tang ethanol extract 20 microg/mL


title: BatchE_ethanol extract of Yijung-tang (Medium dose)_second_sequencing_rep3

description: sample 14-3-2

value: Yijung-tang ethanol extract 100 microg/mL


title: BatchE_ethanol extract of Yijung-tang (Low dose)_second_sequencing_rep3

description: sample 14-3-3

value: Yijung-tang ethanol extract 20 microg/mL


title: BatchE_ethanol extract of Banhasasim-tang (High dose)_second_sequencing_rep3

description: sample 14-3-4

value: Banhasasim-tang ethanol extract 500 microg/mL


title: BatchE_ethanol extract of Yijung-tang (High dose)_second_sequencing_rep3

description: sample 14-3-1

value: Yijung-tang ethanol extract 500 microg/mL


title: BatchE_ethanol extract of Banhasasim-tang (Low dose)_second_sequencing_rep2

description: sample 14-2-6


title: BatchE_ethanol extract of Banhasasim-tang (High dose)_second_sequencing_rep2

description: sample 14-2-4


title: BatchE_Vehicle(DMSO)_second_sequencing_rep2

description: sample E1-2-1

value: 0.1percent DMSO


title: BatchE_Vehicle(DMSO)_second_sequencing_rep1

description: sample E1-1-1


title: BatchE_ethanol extract of Banhasasim-tang (Medium dose)_second_sequencing_rep2

description: sample 14-2-5


title: BatchE_Vehicle(DMSO)_second_sequencing_rep3

description: sample E1-3-1


title: BatchD_ethanol extract of Yijung-tang (Low dose)_second_sequencing_rep2

description: sample 14-2-3


title: BatchD_ethanol extract of Yijung-tang (High dose)_second_sequencing_rep2

description: sample 14-2-1


title: BatchD_ethanol extract of Yijung-tang (Medium dose)_second_sequencing_rep2

description: sample 14-2-2


title: BatchD_ethanol extract of Banhasasim-tang (Low dose)_second_sequencing_rep1

description: sample 14-1-6


title: BatchD_ethanol extract of Banhasasim-tang (Medium dose)_second_sequencing_rep1

description: sample 14-1-5


title: BatchD_ethanol extract of Yijung-tang (Low dose)_second_sequencing_rep1

description: sample 14-1-3


title: BatchD_ethanol extract of Yijung-tang (Medium dose)_second_sequencing_rep1

description: sample 14-1-2


title: BatchD_ethanol extract of Banhasasim-tang (High dose)_second_sequencing_rep1

description: sample 14-1-4


title: BatchD_ethanol extract of Aconiti Lateralis Radix Preparata (Low dose)_second_sequencing_rep3

description: sample 13-3-6

value: Aconiti Lateralis Radix Preparata ethanol extract 20 microg/mL


title: BatchD_ethanol extract of Yijung-tang (High dose)_second_sequencing_rep1

description: sample 14-1-1


title: BatchD_ethanol extract of Aconiti Lateralis Radix Preparata (High dose)_second_sequencing_rep3

description: sample 13-3-4

value: Aconiti Lateralis Radix Preparata ethanol extract 500 microg/mL


title: BatchD_ethanol extract of Aconiti Lateralis Radix Preparata (Medium dose)_second_sequencing_rep3

description: sample 13-3-5

value: Aconiti Lateralis Radix Preparata ethanol extract 100 microg/mL


title: BatchD_ethanol extract of Bupleuri Radix (Low dose)_second_sequencing_rep3

description: sample 13-3-3

value: Bupleuri Radix ethanol extract 8.4 microg/mL


title: BatchD_ethanol extract of Bupleuri Radix (Medium dose)_second_sequencing_rep3

description: sample 13-3-2

value: Bupleuri Radix ethanol extract 42 microg/mL


title: BatchD_ethanol extract of Aconiti Lateralis Radix Preparata (Low dose)_second_sequencing_rep2

description: sample 13-2-6


title: BatchD_ethanol extract of Bupleuri Radix (High dose)_second_sequencing_rep3

description: sample 13-3-1

value: Bupleuri Radix ethanol extract 210 microg/mL


title: BatchD_ethanol extract of Aconiti Lateralis Radix Preparata (Medium dose)_second_sequencing_rep2

description: sample 13-2-5


title: BatchD_ethanol extract of Aconiti Lateralis Radix Preparata (High dose)_second_sequencing_rep2

description: sample 13-2-4


title: BatchD_ethanol extract of Bupleuri Radix (Low dose)_second_sequencing_rep2

description: sample 13-2-3


title: BatchD_ethanol extract of Aconiti Lateralis Radix Preparata (Low dose)_second_sequencing_rep1

description: sample 13-1-6


title: BatchD_ethanol extract of Aconiti Lateralis Radix Preparata (Medium dose)_second_sequencing_rep1

description: sample 13-1-5


title: BatchD_ethanol extract of Aconiti Lateralis Radix Preparata (High dose)_second_sequencing_rep1

description: sample 13-1-4


title: BatchD_ethanol extract of Bupleuri Radix (Medium dose)_second_sequencing_rep2

description: sample 13-2-2


title: BatchD_ethanol extract of Bupleuri Radix (High dose)_second_sequencing_rep2

description: sample 13-2-1


title: BatchD_ethanol extract of Bupleuri Radix (Medium dose)_second_sequencing_rep1

description: sample 13-1-2


title: BatchD_ethanol extract of Bupleuri Radix (High dose)_second_sequencing_rep1

description: sample 13-1-1


title: BatchD_ethanol extract of Bupleuri Radix (Low dose)_second_sequencing_rep1

description: sample 13-1-3


title: BatchD_ethanol extract of Cimicifugae Rhizoma (Medium dose)_second_sequencing_rep3

description: sample 12-3-5

value: Cimicifugae Rhizoma ethanol extract 100 microg/mL


title: BatchD_ethanol extract of Citri Unshius Pericarpium (Low dose)_second_sequencing_rep3

description: sample 12-3-3

value: Citri Unshius Pericarpium ethanol extract 20 microg/mL


title: BatchD_ethanol extract of Citri Unshius Pericarpium (Medium dose)_second_sequencing_rep3

description: sample 12-3-2

value: Citri Unshius Pericarpium ethanol extract 100 microg/mL


title: BatchD_ethanol extract of Citri Unshius Pericarpium (High dose)_second_sequencing_rep3

description: sample 12-3-1

value: Citri Unshius Pericarpium ethanol extract 500 microg/mL


title: BatchD_ethanol extract of Cimicifugae Rhizoma (High dose)_second_sequencing_rep3

description: sample 12-3-4

value: Cimicifugae Rhizoma ethanol extract 500 microg/mL


title: BatchD_ethanol extract of Cimicifugae Rhizoma (Medium dose)_second_sequencing_rep2

description: sample 12-2-5


title: BatchD_ethanol extract of Cimicifugae Rhizoma (High dose)_second_sequencing_rep2

description: sample 12-2-4


title: BatchD_ethanol extract of Citri Unshius Pericarpium (Low dose)_second_sequencing_rep2

description: sample 12-2-3


title: BatchD_ethanol extract of Citri Unshius Pericarpium (Medium dose)_second_sequencing_rep2

description: sample 12-2-2


title: BatchD_ethanol extract of Cimicifugae Rhizoma (Medium dose)_second_sequencing_rep1

description: sample 12-1-5


title: BatchD_ethanol extract of Cimicifugae Rhizoma (Low dose)_second_sequencing_rep1

description: sample 12-1-6

value: Cimicifugae Rhizoma ethanol extract 20 microg/mL


title: BatchD_ethanol extract of Citri Unshius Pericarpium (High dose)_second_sequencing_rep2

description: sample 12-2-1


title: BatchD_ethanol extract of Cimicifugae Rhizoma (Low dose)_second_sequencing_rep2

description: sample 12-2-6


title: BatchD_ethanol extract of Citri Unshius Pericarpium (Low dose)_second_sequencing_rep1

description: sample 12-1-3


title: BatchD_ethanol extract of Cimicifugae Rhizoma (High dose)_second_sequencing_rep1

description: sample 12-1-4


title: BatchD_ethanol extract of Zizyphi Fructus (Low dose)_second_sequencing_rep3

description: sample 11-3-6

value: Zizyphi Fructus ethanol extract 20 microg/mL


title: BatchD_ethanol extract of Citri Unshius Pericarpium (Medium dose)_second_sequencing_rep1

description: sample 12-1-2


title: BatchD_ethanol extract of Zizyphi Fructus (Medium dose)_second_sequencing_rep3

description: sample 11-3-5

value: Zizyphi Fructus ethanol extract 100 microg/mL


title: BatchD_ethanol extract of Zizyphi Fructus (High dose)_second_sequencing_rep3

description: sample 11-3-4

value: Zizyphi Fructus ethanol extract 500 microg/mL


title: BatchD_ethanol extract of Citri Unshius Pericarpium (High dose)_second_sequencing_rep1

description: sample 12-1-1


title: BatchD_ethanol extract of Coptidis Rhizoma (Low dose)_second_sequencing_rep3

description: sample 11-3-3

value: Coptidis Rhizoma ethanol extract 0.56 microg/mL


title: BatchD_ethanol extract of Coptidis Rhizoma (Medium dose)_second_sequencing_rep3

description: sample 11-3-2

value: Coptidis Rhizoma ethanol extract 2.8 microg/mL


title: BatchD_ethanol extract of Coptidis Rhizoma (High dose)_second_sequencing_rep3

description: sample 11-3-1

value: Coptidis Rhizoma ethanol extract 14 microg/mL


title: BatchD_ethanol extract of Zizyphi Fructus (Medium dose)_second_sequencing_rep2

description: sample 11-2-5


title: CUT_and_Tag_Y1F_H2AZ_dTAG_0h_rep1

description:
Library name: sample150
CUT_and_Tag_H2AZ_Y1F_Rawcounts.csv
H2AZ_Y1F_DEseq2_results.csv

value: RPB1 degron, Y1F RPB1 complement

value: Untreated

value: H2A.Z antibody (Abcam #ab4174)


title: BatchD_ethanol extract of Zizyphi Fructus (High dose)_second_sequencing_rep2

description: sample 11-2-4


title: BatchD_ethanol extract of Coptidis Rhizoma (Medium dose)_second_sequencing_rep2

description: sample 11-2-2


title: BatchD_ethanol extract of Cimicifugae Rhizoma (Low dose)_second_sequencing_rep3

description: sample 12-3-6


title: BatchD_ethanol extract of Zizyphi Fructus (Low dose)_second_sequencing_rep2

description: sample 11-2-6


title: BatchD_ethanol extract of Coptidis Rhizoma (Low dose)_second_sequencing_rep2

description: sample 11-2-3


title: BatchD_ethanol extract of Coptidis Rhizoma (High dose)_second_sequencing_rep2

description: sample 11-2-1


title: BatchD_Vehicle(DMSO)_second_sequencing_rep2

description: sample D1-2-1

value: 0.1percent DMSO


title: BatchC_ethanol extract of Zizyphi Fructus (Low dose)_second_sequencing_rep1

description: sample 11-1-6


title: BatchD_Vehicle(DMSO)_second_sequencing_rep3

description: sample D1-3-1


title: BatchD_Vehicle(DMSO)_second_sequencing_rep1

description: sample D1-1-1


title: BatchC_ethanol extract of Zizyphi Fructus (Medium dose)_second_sequencing_rep1

description: sample 11-1-5


title: BatchC_ethanol extract of Coptidis Rhizoma (Medium dose)_second_sequencing_rep1

description: sample 11-1-2


title: BatchC_ethanol extract of Coptidis Rhizoma (Low dose)_second_sequencing_rep1

description: sample 11-1-3


title: BatchC_ethanol extract of Coptidis Rhizoma (High dose)_second_sequencing_rep1

description: sample 11-1-1


title: BatchC_ethanol extract of Scutellariae Radix (Low dose)_second_sequencing_rep3

description: sample 10-3-6

value: Scutellariae Radix ethanol extract 9.2 microg/mL


title: BatchC_ethanol extract of Scutellariae Radix (High dose)_second_sequencing_rep3

description: sample 10-3-4

value: Scutellariae Radix ethanol extract 230 microg/mL


title: BatchC_ethanol extract of Zizyphi Fructus (High dose)_second_sequencing_rep1

description: sample 11-1-4


title: BatchC_ethanol extract of Scutellariae Radix (Medium dose)_second_sequencing_rep3

description: sample 10-3-5

value: Scutellariae Radix ethanol extract 46 microg/mL


title: BatchC_ethanol extract of Pinelliae Tuber (Low dose)_second_sequencing_rep3

description: sample 10-3-3

value: Pinelliae Tuber ethanol extract 20 microg/mL


title: BatchC_ethanol extract of Pinelliae Tuber (Medium dose)_second_sequencing_rep3

description: sample 10-3-2

value: Pinelliae Tuber ethanol extract 100 microg/mL


title: BatchC_ethanol extract of Pinelliae Tuber (High dose)_second_sequencing_rep3

description: sample 10-3-1

value: Pinelliae Tuber ethanol extract 500 microg/mL


title: BatchC_ethanol extract of Scutellariae Radix (Low dose)_second_sequencing_rep1

description: sample 10-1-6


title: BatchC_ethanol extract of Pinelliae Tuber (Medium dose)_second_sequencing_rep2

description: sample 10-2-2


title: BatchC_ethanol extract of Scutellariae Radix (High dose)_second_sequencing_rep2

description: sample 10-2-4


title: BatchC_ethanol extract of Scutellariae Radix (Medium dose)_second_sequencing_rep2

description: sample 10-2-5


title: BatchC_ethanol extract of Pinelliae Tuber (Low dose)_second_sequencing_rep2

description: sample 10-2-3


title: BatchC_ethanol extract of Scutellariae Radix (Medium dose)_second_sequencing_rep1

description: sample 10-1-5


title: BatchC_ethanol extract of Pinelliae Tuber (Low dose)_second_sequencing_rep1

description: sample 10-1-3


title: BatchC_ethanol extract of Scutellariae Radix (High dose)_second_sequencing_rep1

description: sample 10-1-4


title: BatchC_ethanol extract of Pinelliae Tuber (Medium dose)_second_sequencing_rep1

description: sample 10-1-2


title: BatchC_ethanol extract of Pinelliae Tuber (High dose)_second_sequencing_rep1

description: sample 10-1-1


title: BatchC_ethanol extract of Zingiberis Rhizoma (Low dose)_second_sequencing_rep3

description: sample 9-3-6

value: Zingiberis Rhizoma ethanol extract 20 microg/mL


title: BatchC_ethanol extract of Zingiberis Rhizoma (Medium dose)_second_sequencing_rep3

description: sample 9-3-5

value: Zingiberis Rhizoma ethanol extract 100 microg/mL


title: BatchC_ethanol extract of Zingiberis Rhizoma Recens (Low dose)_second_sequencing_rep3

description: sample 9-3-3

value: Zingiberis Rhizoma Recens ethanol extract 20 microg/mL


title: BatchC_ethanol extract of Zingiberis Rhizoma Recens (High dose)_second_sequencing_rep3

description: sample 9-3-1

value: Zingiberis Rhizoma Recens ethanol extract 500 microg/mL


title: BatchC_ethanol extract of Zingiberis Rhizoma (Low dose)_second_sequencing_rep2

description: sample 9-2-6


title: BatchC_ethanol extract of Zingiberis Rhizoma (Medium dose)_second_sequencing_rep2

description: sample 9-2-5


title: BatchC_ethanol extract of Zingiberis Rhizoma Recens (Medium dose)_second_sequencing_rep3

description: sample 9-3-2

value: Zingiberis Rhizoma Recens ethanol extract 100 microg/mL


title: BatchC_ethanol extract of Zingiberis Rhizoma (High dose)_second_sequencing_rep2

description: sample 9-2-4

value: Zingiberis Rhizoma ethanol extract 500 microg/mL


title: BatchC_ethanol extract of Zingiberis Rhizoma Recens (High dose)_second_sequencing_rep2

description: sample 9-2-1


title: BatchC_ethanol extract of Zingiberis Rhizoma Recens (Medium dose)_second_sequencing_rep2

description: sample 9-2-2


title: BatchC_ethanol extract of Zingiberis Rhizoma (Low dose)_second_sequencing_rep1

description: sample 9-1-6


title: BatchC_ethanol extract of Zingiberis Rhizoma (High dose)_second_sequencing_rep1

description: sample 9-1-4


title: BatchC_ethanol extract of Zingiberis Rhizoma Recens (Low dose)_second_sequencing_rep1

description: sample 9-1-3


title: BatchC_ethanol extract of Zingiberis Rhizoma (Medium dose)_second_sequencing_rep1

description: sample 9-1-5


title: BatchC_ethanol extract of Scutellariae Radix (Low dose)_second_sequencing_rep2

description: sample 10-2-6


title: BatchC_ethanol extract of Zingiberis Rhizoma Recens (Medium dose)_second_sequencing_rep1

description: sample 9-1-2


title: BatchC_ethanol extract of Pinelliae Tuber (High dose)_second_sequencing_rep2

description: sample 10-2-1


title: BatchC_water extract of Sayeok-tang (Low dose)_second_sequencing_rep3

description: sample 8-3-6

value: Sayeok-tang water extract 20 microg/mL


title: BatchC_ethanol extract of Zingiberis Rhizoma Recens (High dose)_second_sequencing_rep1

description: sample 9-1-1


title: BatchC_water extract of Sayeok-tang (Medium dose)_second_sequencing_rep3

description: sample 8-3-5

value: Sayeok-tang water extract 100 microg/mL


title: BatchC_water extract of Sayeok-tang (High dose)_second_sequencing_rep3

description: sample 8-3-4

value: Sayeok-tang water extract 500 microg/mL


title: BatchC_water extract of Bojungikgi-tang (Medium dose)_second_sequencing_rep3

description: sample 8-3-2

value: Bojungikgi-tang water extract 100 microg/mL


title: BatchC_water extract of Bojungikgi-tang (High dose)_second_sequencing_rep3

description: sample 8-3-1

value: Bojungikgi-tang water extract 500 microg/mL


title: BatchC_water extract of Bojungikgi-tang (Low dose)_second_sequencing_rep3

description: sample 8-3-3

value: Bojungikgi-tang water extract 20 microg/mL


title: BatchC_water extract of Sayeok-tang (Low dose)_second_sequencing_rep2

description: sample 8-2-6


title: BatchC_water extract of Sayeok-tang (Medium dose)_second_sequencing_rep2

description: sample 8-2-5


title: BatchC_water extract of Sayeok-tang (High dose)_second_sequencing_rep2

description: sample 8-2-4


title: BatchC_water extract of Bojungikgi-tang (Medium dose)_second_sequencing_rep2

description: sample 8-2-2


title: BatchC_water extract of Sayeok-tang (Medium dose)_second_sequencing_rep1

description: sample 8-1-5


title: BatchC_water extract of Bojungikgi-tang (High dose)_second_sequencing_rep2

description: sample 8-2-1


title: BatchC_water extract of Sayeok-tang (High dose)_second_sequencing_rep1

description: sample 8-1-4


title: BatchC_ethanol extract of Zingiberis Rhizoma Recens (Low dose)_second_sequencing_rep2

description: sample 9-2-3


title: BatchC_Vehicle(DMSO)_second_sequencing_rep3

description: sample C1-3-1

value: 0.1percent DMSO


title: BatchC_water extract of Sayeok-tang (Low dose)_second_sequencing_rep1

description: sample 8-1-6


title: BatchC_Vehicle(DMSO)_second_sequencing_rep2

description: sample C1-2-1


title: BatchB_water extract of Bojungikgi-tang (Low dose)_second_sequencing_rep1

description: sample 8-1-3


title: BatchB_water extract of Bojungikgi-tang (High dose)_second_sequencing_rep1

description: sample 8-1-1


title: BatchB_water extract of Bojungikgi-tang (Medium dose)_second_sequencing_rep1

description: sample 8-1-2


title: BatchB_water extract of Banhasasim-tang (Low dose)_second_sequencing_rep3

description: sample 7-3-6

value: Banhasasim-tang water extract 20 microg/mL


title: BatchB_water extract of Banhasasim-tang (Medium dose)_second_sequencing_rep3

description: sample 7-3-5

value: Banhasasim-tang water extract 100 microg/mL


title: BatchB_water extract of Banhasasim-tang (High dose)_second_sequencing_rep3

description: sample 7-3-4

value: Banhasasim-tang water extract 500 microg/mL


title: BatchB_water extract of Yijung-tang (Medium dose)_second_sequencing_rep3

description: sample 7-3-2

value: Yijung-tang water extract 100 microg/mL


title: BatchB_water extract of Yijung-tang (Low dose)_second_sequencing_rep3

description: sample 7-3-3

value: Yijung-tang water extract 20 microg/mL


title: BatchB_water extract of Yijung-tang (High dose)_second_sequencing_rep3

description: sample 7-3-1

value: Yijung-tang water extract 500 microg/mL


title: BatchB_water extract of Banhasasim-tang (Low dose)_second_sequencing_rep2

description: sample 7-2-6


title: BatchB_water extract of Banhasasim-tang (Medium dose)_second_sequencing_rep2

description: sample 7-2-5


title: BatchB_water extract of Banhasasim-tang (High dose)_second_sequencing_rep2

description: sample 7-2-4


title: BatchB_water extract of Yijung-tang (Medium dose)_second_sequencing_rep2

description: sample 7-2-2


title: BatchB_water extract of Yijung-tang (Low dose)_second_sequencing_rep2

description: sample 7-2-3


title: BatchB_water extract of Yijung-tang (High dose)_second_sequencing_rep2

description: sample 7-2-1


title: BatchB_water extract of Banhasasim-tang (Low dose)_second_sequencing_rep1

description: sample 7-1-6


title: BatchC_water extract of Bojungikgi-tang (Low dose)_second_sequencing_rep2

description: sample 8-2-3


title: BatchB_water extract of Banhasasim-tang (Medium dose)_second_sequencing_rep1

description: sample 7-1-5


title: BatchB_water extract of Yijung-tang (Medium dose)_second_sequencing_rep1

description: sample 7-1-2


title: BatchB_water extract of Banhasasim-tang (High dose)_second_sequencing_rep1

description: sample 7-1-4


title: BatchB_water extract of Yijung-tang (Low dose)_second_sequencing_rep1

description: sample 7-1-3


title: BatchB_water extract of Yijung-tang (High dose)_second_sequencing_rep1

description: sample 7-1-1


title: BatchB_water extract of Aconiti Lateralis Radix Preparata (Low dose)_second_sequencing_rep3

description: sample 6-3-6

value: Aconiti Lateralis Radix Preparata water extract 20 microg/mL


title: BatchB_water extract of Aconiti Lateralis Radix Preparata (High dose)_second_sequencing_rep3

description: sample 6-3-4

value: Aconiti Lateralis Radix Preparata water extract 500 microg/mL


title: BatchB_water extract of Bupleuri Radix (Low dose)_second_sequencing_rep3

description: sample 6-3-3

value: Bupleuri Radix water extract 20 microg/mL


title: BatchB_water extract of Bupleuri Radix (Medium dose)_second_sequencing_rep3

description: sample 6-3-2

value: Bupleuri Radix water extract 100 microg/mL


title: BatchB_water extract of Bupleuri Radix (High dose)_second_sequencing_rep3

description: sample 6-3-1

value: Bupleuri Radix water extract 500 microg/mL


title: BatchC_Vehicle(DMSO)_second_sequencing_rep1

description: sample C1-1-1


title: BatchB_water extract of Aconiti Lateralis Radix Preparata (Medium dose)_second_sequencing_rep3

description: sample 6-3-5

value: Aconiti Lateralis Radix Preparata water extract 100 microg/mL


title: BatchB_water extract of Aconiti Lateralis Radix Preparata (Medium dose)_second_sequencing_rep2

description: sample 6-2-5


title: BatchB_water extract of Aconiti Lateralis Radix Preparata (Low dose)_second_sequencing_rep2

description: sample 6-2-6


title: BatchB_water extract of Aconiti Lateralis Radix Preparata (High dose)_second_sequencing_rep2

description: sample 6-2-4


title: BatchB_water extract of Bupleuri Radix (Low dose)_second_sequencing_rep2

description: sample 6-2-3


title: BatchB_water extract of Bupleuri Radix (Medium dose)_second_sequencing_rep2

description: sample 6-2-2


title: BatchB_water extract of Bupleuri Radix (High dose)_second_sequencing_rep2

description: sample 6-2-1


title: BatchB_water extract of Aconiti Lateralis Radix Preparata (High dose)_second_sequencing_rep1

description: sample 6-1-4


title: BatchB_water extract of Aconiti Lateralis Radix Preparata (Medium dose)_second_sequencing_rep1

description: sample 6-1-5


title: BatchB_water extract of Aconiti Lateralis Radix Preparata (Low dose)_second_sequencing_rep1

description: sample 6-1-6


title: BatchB_water extract of Bupleuri Radix (Low dose)_second_sequencing_rep1

description: sample 6-1-3


title: BatchB_water extract of Bupleuri Radix (Medium dose)_second_sequencing_rep1

description: sample 6-1-2


title: BatchB_water extract of Bupleuri Radix (High dose)_second_sequencing_rep1

description: sample 6-1-1


title: BatchB_water extract of  Cimicifugae Rhizoma (Low dose)_second_sequencing_rep3

description: sample 5-3-6

value: Cimicifugae Rhizoma water extract 20 microg/mL


title: BatchB_water extract of  Cimicifugae Rhizoma (High dose)_second_sequencing_rep3

description: sample 5-3-4

value: Cimicifugae Rhizoma water extract 500 microg/mL


title: BatchB_water extract of  Cimicifugae Rhizoma (Medium dose)_second_sequencing_rep3

description: sample 5-3-5

value: Cimicifugae Rhizoma water extract 100 microg/mL


title: BatchB_water extract of Citri Unshius Pericarpium (Medium dose)_second_sequencing_rep3

description: sample 5-3-2

value: Citri Unshius Pericarpium water extract 100 microg/mL


title: BatchB_water extract of Citri Unshius Pericarpium (Low dose)_second_sequencing_rep3

description: sample 5-3-3

value: Citri Unshius Pericarpium water extract 20 microg/mL


title: BatchB_water extract of Citri Unshius Pericarpium (High dose)_second_sequencing_rep3

description: sample 5-3-1

value: Citri Unshius Pericarpium water extract 500 microg/mL


title: BatchB_water extract of  Cimicifugae Rhizoma (Medium dose)_second_sequencing_rep2

description: sample 5-2-5


title: BatchB_water extract of Citri Unshius Pericarpium (Low dose)_second_sequencing_rep2

description: sample 5-2-3


title: BatchB_water extract of  Cimicifugae Rhizoma (High dose)_second_sequencing_rep2

description: sample 5-2-4


title: BatchB_water extract of  Cimicifugae Rhizoma (Low dose)_second_sequencing_rep2

description: sample 5-2-6


title: BatchB_water extract of Citri Unshius Pericarpium (High dose)_second_sequencing_rep2

description: sample 5-2-1


title: BatchB_water extract of  Cimicifugae Rhizoma (Low dose)_second_sequencing_rep1

description: sample 5-1-6


title: BatchB_water extract of  Cimicifugae Rhizoma (Medium dose)_second_sequencing_rep1

description: sample 5-1-5


title: BatchB_water extract of Citri Unshius Pericarpium (Medium dose)_second_sequencing_rep2

description: sample 5-2-2


title: BatchB_water extract of  Cimicifugae Rhizoma (High dose)_second_sequencing_rep1

description: sample 5-1-4


title: BatchB_water extract of Citri Unshius Pericarpium (Low dose)_second_sequencing_rep1

description: sample 5-1-3


title: BatchB_water extract of Citri Unshius Pericarpium (Medium dose)_second_sequencing_rep1

description: sample 5-1-2


title: BatchB_Vehicle(DMSO)_second_sequencing_rep3

description: sample B1-3-1

value: 0.1percent DMSO


title: BatchB_Vehicle(DMSO)_second_sequencing_rep1

description: sample B1-1-1


title: BatchA_water extract of Zizyphi Fructus (Low dose)_second_sequencing_rep3

description: sample 4-3-6

value: Zizyphi Fructus water extract 20 microg/mL


title: BatchB_Vehicle(DMSO)_second_sequencing_rep2

description: sample B1-2-1


title: BatchA_water extract of Zizyphi Fructus (Medium dose)_second_sequencing_rep3

description: sample 4-3-5

value: Zizyphi Fructus water extract 100 microg/mL


title: BatchA_water extract of Zizyphi Fructus (High dose)_second_sequencing_rep3

description: sample 4-3-4

value: Zizyphi Fructus water extract 500 microg/mL


title: BatchA_water extract of Coptidis Rhizoma (Low dose)_second_sequencing_rep3

description: sample 4-3-3

value: Coptidis Rhizoma water extract 0.8 microg/mL


title: BatchA_water extract of Zizyphi Fructus (Low dose)_second_sequencing_rep2

description: sample 4-2-6


title: BatchA_water extract of Coptidis Rhizoma (Medium dose)_second_sequencing_rep3

description: sample 4-3-2

value: Coptidis Rhizoma water extract 4 microg/mL


title: BatchA_water extract of Coptidis Rhizoma (High dose)_second_sequencing_rep3

description: sample 4-3-1

value: Coptidis Rhizoma water extract 20 microg/mL


title: BatchA_water extract of Zizyphi Fructus (Medium dose)_second_sequencing_rep2

description: sample 4-2-5


title: BatchA_water extract of Coptidis Rhizoma (High dose)_second_sequencing_rep2

description: sample 4-2-1


title: BatchA_water extract of Zizyphi Fructus (Medium dose)_second_sequencing_rep1

description: sample 4-1-5


title: BatchA_water extract of Zizyphi Fructus (Low dose)_second_sequencing_rep1

description: sample 4-1-6


title: BatchA_water extract of Coptidis Rhizoma (Medium dose)_second_sequencing_rep1

description: sample 4-1-2


title: BatchA_water extract of Coptidis Rhizoma (Low dose)_second_sequencing_rep1

description: sample 4-1-3


title: BatchA_water extract of Zizyphi Fructus (High dose)_second_sequencing_rep1

description: sample 4-1-4


title: BatchA_water extract of Scutellariae Radix (Low dose)_second_sequencing_rep3

description: sample 3-3-6

value: Scutellariae Radix water extract 16.4 microg/mL


title: BatchB_water extract of Citri Unshius Pericarpium (High dose)_second_sequencing_rep1

description: sample 5-1-1


title: BatchA_water extract of Scutellariae Radix (High dose)_second_sequencing_rep3

description: sample 3-3-4

value: Scutellariae Radix water extract 410 microg/mL


title: BatchA_water extract of Pinelliae Tuber (Medium dose)_second_sequencing_rep3

description: sample 3-3-2

value: Pinelliae Tuber water extract 100 microg/mL


title: BatchA_water extract of Scutellariae Radix (Medium dose)_second_sequencing_rep3

description: sample 3-3-5

value: Scutellariae Radix water extract 82 microg/mL


title: BatchA_water extract of Pinelliae Tuber (Low dose)_second_sequencing_rep3

description: sample 3-3-3

value: Pinelliae Tuber water extract 20 microg/mL


title: BatchA_water extract of Scutellariae Radix (Medium dose)_second_sequencing_rep2

description: sample 3-2-5


title: BatchA_water extract of Pinelliae Tuber (Low dose)_second_sequencing_rep2

description: sample 3-2-3


title: BatchA_water extract of Pinelliae Tuber (High dose)_second_sequencing_rep2

description: sample 3-2-1

value: Pinelliae Tuber water extract 500 microg/mL


title: BatchA_water extract of Scutellariae Radix (Low dose)_second_sequencing_rep1

description: sample 3-1-6


title: BatchA_water extract of Scutellariae Radix (Medium dose)_second_sequencing_rep1

description: sample 3-1-5


title: BatchA_water extract of Scutellariae Radix (High dose)_second_sequencing_rep1

description: sample 3-1-4


title: BatchA_water extract of Coptidis Rhizoma (Low dose)_second_sequencing_rep2

description: sample 4-2-3


title: BatchA_water extract of Pinelliae Tuber (Medium dose)_second_sequencing_rep1

description: sample 3-1-2


title: BatchA_water extract of Coptidis Rhizoma (Medium dose)_second_sequencing_rep2

description: sample 4-2-2


title: BatchA_water extract of Zizyphi Fructus (High dose)_second_sequencing_rep2

description: sample 4-2-4


title: BatchA_water extract of Pinelliae Tuber (Low dose)_second_sequencing_rep1

description: sample 3-1-3


title: BatchA_water extract of Coptidis Rhizoma (High dose)_second_sequencing_rep1

description: sample 4-1-1


title: BatchA_water extract of Pinelliae Tuber (High dose)_second_sequencing_rep1

description: sample 3-1-1


title: BatchA_water extract of Zingiberis Rhizoma Recens (Low dose)_second_sequencing_rep3

description: sample 2-3-3

value: Zingiberis Rhizoma Recens water extract 20 microg/mL


title: BatchA_water extract of Zingiberis Rhizoma Recens (High dose)_second_sequencing_rep3

description: sample 2-3-1

value: Zingiberis Rhizoma Recens water extract 500 microg/mL


title: BatchA_water extract of Zingiberis Rhizoma (Low dose)_second_sequencing_rep3

description: sample 2-3-6

value: Zingiberis Rhizoma water extract 20 microg/mL


title: BatchA_water extract of Zingiberis Rhizoma (Low dose)_second_sequencing_rep2

description: sample 2-2-6


title: BatchA_water extract of Zingiberis Rhizoma (Medium dose)_second_sequencing_rep3

description: sample 2-3-5

value: Zingiberis Rhizoma water extract 100 microg/mL


title: BatchA_water extract of Zingiberis Rhizoma Recens (Medium dose)_second_sequencing_rep3

description: sample 2-3-2

value: Zingiberis Rhizoma Recens water extract 100 microg/mL


title: BatchA_water extract of Scutellariae Radix (Low dose)_second_sequencing_rep2

description: sample 3-2-6


title: BatchA_water extract of Zingiberis Rhizoma (High dose)_second_sequencing_rep2

description: sample 2-2-4

value: Zingiberis Rhizoma water extract 500 microg/mL


title: BatchA_water extract of Pinelliae Tuber (High dose)_second_sequencing_rep3

description: sample 3-3-1


title: BatchA_water extract of Zingiberis Rhizoma Recens (Low dose)_second_sequencing_rep2

description: sample 2-2-3


title: BatchA_water extract of Zingiberis Rhizoma (Low dose)_second_sequencing_rep1

description: sample 2-1-6


title: BatchA_water extract of Zingiberis Rhizoma Recens (High dose)_second_sequencing_rep2

description: sample 2-2-1


title: BatchA_water extract of Zingiberis Rhizoma Recens (Medium dose)_second_sequencing_rep2

description: sample 2-2-2


title: BatchA_water extract of Zingiberis Rhizoma (High dose)_second_sequencing_rep3

description: sample 2-3-4


title: BatchA_water extract of Zingiberis Rhizoma (Medium dose)_second_sequencing_rep1

description: sample 2-1-5


title: BatchA_water extract of Scutellariae Radix (High dose)_second_sequencing_rep2

description: sample 3-2-4


title: BatchA_water extract of Pinelliae Tuber (Medium dose)_second_sequencing_rep2

description: sample 3-2-2


title: BatchA_water extract of Zingiberis Rhizoma (High dose)_second_sequencing_rep1

description: sample 2-1-4


title: BatchA_water extract of Zingiberis Rhizoma Recens (Low dose)_second_sequencing_rep1

description: sample 2-1-3


title: BatchA_water extract of Zingiberis Rhizoma Recens (High dose)_second_sequencing_rep1

description: sample 2-1-1


title: BatchA_Wortmannin_second_sequencing_rep1

description: sample 1-1-4

value: wortmannin 10 uM


title: BatchA_Wortmannin_second_sequencing_rep2

description: sample 1-2-4


title: BatchA_Wortmannin_second_sequencing_rep3

description: sample 1-3-4


title: BatchA_Vehicle(DMSO)_second_sequencing_rep3

description: sample A1-3-1

value: 0.1percent DMSO


title: BatchA_Vehicle(DMSO)_second_sequencing_rep2

description: sample A1-2-1


title: BatchA_Vehicle(DMSO)_second_sequencing_rep1

description: sample A1-1-1


title: A549_Second_BatchE_ethanol extract of Sayeok-tang (Low dose)_rep3

description: sample 15-3-6

growth_protocol: Human non-small cell lung carcinoma, A549 (CCL-185) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). A549 cells were maintained in RPMI medium supplemented with 10% (v/v) heat-inactivated FBS, 100 IU/mL penicillin, and 100 mg/mL streptomycin at 37 °C, 5% CO2 incubator. The cell was subcultured every 3 or 4 days, depending on the cell density.

treatment_protocol: Roswell Park Memorial Institute (RPMI) 1640 medium, phosphate-buffered saline (PBS), TrypLE Express, penicillin-streptomycin, and fetal bovine serum (FBS) were purchased from Gibco (Grand Island, NY, USA). Cell culture flasks and multiwell culture plates were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Dimethyl sulfoxide (DMSO) and QIAzol lysis reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA) and Qiagen (Germantown, MD, USA), respectively. The dried medicinal plants, prepared in compliance with the Korean Pharmacopoeia, were supplied from Kwangmyung-dang Medicinal Herbs Co. (Ulsan, Republic of Korea). The samples used in the experiment underwent organoleptic examination by Dr. Choi Goya, an herbal medicine organoleptic examination expert appointed by the Korea Food and Drug Administration, and were additionally identified at the species level by analyzing the DNA barcode region sequences. Voucher specimen were deposited in the Korean Herbarium of Standard Herbal Resources, Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine, Naju, Republic of Korea . All herbs and extracts can be obtained from the Oriental Medicine Resources Research Center (KIOM, https://oasis.kiom.re.kr/herblib). A hot water and 70% ethanol extract of each plant was prepared and supplied by KOC Biotech Co. (Daejeon, Republic of Korea). Briefly, dried plants (1,000 g) were pulverized and extracted in hot distilled water (10 L) for 3 h under a reflux extraction system (MS-DM609; MTOPS, Seoul, Republic of Korea) or in 70% ethanol (15L) for 1 h under ultrasonication system (VCP-20, Lab companion, Dajeon, Republic of Korea) twice. The extract solution was filtered through a 5 µm cartridge filter, concentrated in a rotary evaporator (Ev-1020, SciLab, Seoul, Republic of Korea), and then lyophilized in a freeze dryer (LP-20, Ilshin-Bio-Base, Dongduchen, Republic of Korea) to obtain the final extract. The extract was finely homogenized and packed in a glass bottle containing a desiccant silica gel. The decoction was prepared by mixing each extract, considering the composition ratio and extract yield of a single medicinal herb, and homogenized, according to the Korean Pharmacopoeia. For the in vitro treatment, extracts (100 mg) were vigorously vortexed for 30 min in 10 mL phosphate-buffered saline (PBS; Thermo Fisher Scientific, Rockford, IL, USA) containing 2% DMSO and then sterilized by filtering through a 0.22 µm membrane to obtain a stock solution (10 mg/mL); the stock solution was aliquoted in small volumes and stored at -80°C until use.

value: Sayeok-tang ethanol extract 20 μg/mL


title: BatchA_water extract of Zingiberis Rhizoma (Medium dose)_second_sequencing_rep2

description: sample 2-2-5


title: A549_Second_BatchE_ethanol extract of Sayeok-tang (Medium dose)_rep3

description: sample 15-3-5

value: Sayeok-tang ethanol extract 100 μg/mL


title: A549_Second_BatchE_ethanol extract of Sayeok-tang (High dose)_rep3

description: sample 15-3-4

value: Sayeok-tang ethanol extract 500 μg/mL


title: A549_Second_BatchE_ethanol extract of Bojungikgi-tang (Low dose)_rep3

description: sample 15-3-3

value: Bojungikgi-tang ethanol extract 20 μg/mL


title: A549_Second_BatchE_ethanol extract of Bojungikgi-tang (Medium dose)_rep3

description: sample 15-3-2

value: Bojungikgi-tang ethanol extract 100 μg/mL


title: A549_Second_BatchE_ethanol extract of Bojungikgi-tang (High dose)_rep3

description: sample 15-3-1

value: Bojungikgi-tang ethanol extract 500 μg/mL


title: A549_Second_BatchE_ethanol extract of Sayeok-tang (High dose)_rep2

description: sample 15-2-4


title: A549_Second_BatchE_ethanol extract of Bojungikgi-tang (Low dose)_rep2

description: sample 15-2-3


title: A549_Second_BatchE_ethanol extract of Bojungikgi-tang (Medium dose)_rep2

description: sample 15-2-2


title: A549_Second_BatchE_ethanol extract of Bojungikgi-tang (High dose)_rep2

description: sample 15-2-1


title: A549_Second_BatchE_ethanol extract of Sayeok-tang (Low dose)_rep1

description: sample 15-1-6


title: A549_Second_BatchE_ethanol extract of Sayeok-tang (Medium dose)_rep2

description: sample 15-2-5


title: A549_Second_BatchE_ethanol extract of Bojungikgi-tang (Low dose)_rep1

description: sample 15-1-3


title: A549_Second_BatchE_ethanol extract of Bojungikgi-tang (Medium dose)_rep1

description: sample 15-1-2


title: A549_Second_BatchE_ethanol extract of Sayeok-tang (High dose)_rep1

description: sample 15-1-4


title: A549_Second_BatchE_ethanol extract of Banhasasim-tang (Low dose)_rep3

description: sample 14-3-6

value: Banhasasim-tang ethanol extract 20 μg/mL


title: A549_Second_BatchE_ethanol extract of Banhasasim-tang (High dose)_rep3

description: sample 14-3-4

value: Banhasasim-tang ethanol extract 500 μg/mL


title: BatchA_water extract of Zingiberis Rhizoma Recens (Medium dose)_second_sequencing_rep1

description: sample 2-1-2


title: A549_Second_BatchE_ethanol extract of Bojungikgi-tang (High dose)_rep1

description: sample 15-1-1


title: A549_Second_BatchE_ethanol extract of Yijung-tang (Low dose)_rep3

description: sample 14-3-3

value: Yijung-tang ethanol extract 20 μg/mL


title: A549_Second_BatchE_ethanol extract of Banhasasim-tang (Low dose)_rep2

description: sample 14-2-6


title: A549_Second_BatchE_ethanol extract of Yijung-tang (High dose)_rep3

description: sample 14-3-1

value: Yijung-tang ethanol extract 500 μg/mL


title: A549_Second_BatchE_ethanol extract of Yijung-tang (Medium dose)_rep3

description: sample 14-3-2

value: Yijung-tang ethanol extract 100 μg/mL


title: A549_Second_BatchE_ethanol extract of Banhasasim-tang (High dose)_rep2

description: sample 14-2-4


title: A549_Second_BatchE_ethanol extract of Banhasasim-tang (Medium dose)_rep2

description: sample 14-2-5

value: Banhasasim-tang ethanol extract 100 μg/mL


title: A549_Second_BatchE_Vehicle(DMSO)_rep2

description: sample E1-2-1

value: 0.1% DMSO


title: A549_Second_BatchE_Vehicle(DMSO)_rep1

description: sample E1-1-1


title: A549_Second_BatchE_ethanol extract of Sayeok-tang (Low dose)_rep2

description: sample 15-2-6


title: A549_Second_BatchD_ethanol extract of Yijung-tang (Medium dose)_rep2

description: sample 14-2-2


title: A549_Second_BatchD_ethanol extract of Banhasasim-tang (Low dose)_rep1

description: sample 14-1-6


title: A549_Second_BatchD_ethanol extract of Yijung-tang (High dose)_rep2

description: sample 14-2-1


title: A549_Second_BatchE_ethanol extract of Sayeok-tang (Medium dose)_rep1

description: sample 15-1-5


title: A549_Second_BatchD_ethanol extract of Banhasasim-tang (High dose)_rep1

description: sample 14-1-4


title: A549_Second_BatchD_ethanol extract of Banhasasim-tang (Medium dose)_rep1

description: sample 14-1-5


title: A549_Second_BatchD_ethanol extract of Yijung-tang (Low dose)_rep1

description: sample 14-1-3


title: A549_Second_BatchD_ethanol extract of Yijung-tang (Medium dose)_rep1

description: sample 14-1-2


title: A549_Second_BatchD_ethanol extract of Bupleuri Radix (Medium dose)_rep3

description: sample 13-3-2

value: Bupleuri Radix ethanol extract 38 μg/mL


title: A549_Second_BatchE_ethanol extract of Banhasasim-tang (Medium dose)_rep3

description: sample 14-3-5


title: A549_Second_BatchD_ethanol extract of Aconiti Lateralis Radix Preparata (Low dose)_rep3

description: sample 13-3-6

value: Aconiti Lateralis Radix Preparata ethanol extract 20 μg/mL


title: A549_Second_BatchD_ethanol extract of Bupleuri Radix (High dose)_rep3

description: sample 13-3-1

value: Bupleuri Radix ethanol extract 190 μg/mL


title: A549_Second_BatchD_ethanol extract of Aconiti Lateralis Radix Preparata (Low dose)_rep2

description: sample 13-2-6


title: A549_Second_BatchD_ethanol extract of Aconiti Lateralis Radix Preparata (High dose)_rep2

description: sample 13-2-4

value: Aconiti Lateralis Radix Preparata ethanol extract 500 μg/mL


title: A549_Second_BatchD_ethanol extract of Bupleuri Radix (Low dose)_rep2

description: sample 13-2-3

value: Bupleuri Radix ethanol extract 7.6 μg/mL


title: A549_Second_BatchD_ethanol extract of Aconiti Lateralis Radix Preparata (Medium dose)_rep1

description: sample 13-1-5

value: Aconiti Lateralis Radix Preparata ethanol extract 100 μg/mL


title: A549_Second_BatchD_ethanol extract of Aconiti Lateralis Radix Preparata (Low dose)_rep1

description: sample 13-1-6


title: A549_Second_BatchE_Vehicle(DMSO)_rep3

description: sample E1-3-1


title: A549_Second_BatchD_ethanol extract of Yijung-tang (Low dose)_rep2

description: sample 14-2-3


title: A549_Second_BatchD_ethanol extract of Aconiti Lateralis Radix Preparata (High dose)_rep1

description: sample 13-1-4


title: A549_Second_BatchD_ethanol extract of Bupleuri Radix (Low dose)_rep1

description: sample 13-1-3


title: A549_Second_BatchD_ethanol extract of Bupleuri Radix (Medium dose)_rep1

description: sample 13-1-2


title: A549_Second_BatchD_ethanol extract of Cimicifugae Rhizoma (Low dose)_rep3

description: sample 12-3-6

value: Cimicifugae Rhizoma ethanol extract 20 μg/mL


title: A549_Second_BatchD_ethanol extract of Yijung-tang (High dose)_rep1

description: sample 14-1-1


title: A549_Second_BatchD_ethanol extract of Aconiti Lateralis Radix Preparata (High dose)_rep3

description: sample 13-3-4


title: A549_Second_BatchD_ethanol extract of Cimicifugae Rhizoma (Low dose)_rep2

description: sample 12-2-6


title: A549_Second_BatchD_ethanol extract of Citri Unshius Pericarpium (Medium dose)_rep3

description: sample 12-3-2

value: Citri Unshius Pericarpium ethanol extract 100 μg/mL


title: A549_Second_BatchD_ethanol extract of Citri Unshius Pericarpium (Low dose)_rep3

description: sample 12-3-3

value: Citri Unshius Pericarpium ethanol extract 20 μg/mL


title: A549_Second_BatchD_ethanol extract of Bupleuri Radix (High dose)_rep1

description: sample 13-1-1


title: A549_Second_BatchD_ethanol extract of Cimicifugae Rhizoma (High dose)_rep3

description: sample 12-3-4

value: Cimicifugae Rhizoma ethanol extract 500 μg/mL


title: A549_Second_BatchD_ethanol extract of Citri Unshius Pericarpium (High dose)_rep3

description: sample 12-3-1

value: Citri Unshius Pericarpium ethanol extract 500 μg/mL


title: A549_Second_BatchD_ethanol extract of Bupleuri Radix (Low dose)_rep3

description: sample 13-3-3


title: A549_Second_BatchD_ethanol extract of Cimicifugae Rhizoma (Medium dose)_rep2

description: sample 12-2-5

value: Cimicifugae Rhizoma ethanol extract 100 μg/mL


title: A549_Second_BatchD_ethanol extract of Citri Unshius Pericarpium (Low dose)_rep2

description: sample 12-2-3


title: A549_Second_BatchD_ethanol extract of Citri Unshius Pericarpium (High dose)_rep2

description: sample 12-2-1


title: A549_Second_BatchD_ethanol extract of Cimicifugae Rhizoma (High dose)_rep1

description: sample 12-1-4


title: A549_Second_BatchD_ethanol extract of Cimicifugae Rhizoma (Medium dose)_rep1

description: sample 12-1-5


title: A549_Second_BatchD_ethanol extract of Cimicifugae Rhizoma (High dose)_rep2

description: sample 12-2-4


title: A549_Second_BatchD_ethanol extract of Citri Unshius Pericarpium (Medium dose)_rep2

description: sample 12-2-2


title: A549_Second_BatchD_ethanol extract of Bupleuri Radix (Medium dose)_rep2

description: sample 13-2-2


title: A549_Second_BatchD_ethanol extract of Aconiti Lateralis Radix Preparata (Medium dose)_rep2

description: sample 13-2-5


title: A549_Second_BatchD_ethanol extract of Cimicifugae Rhizoma (Low dose)_rep1

description: sample 12-1-6


title: A549_Second_BatchD_ethanol extract of Citri Unshius Pericarpium (Low dose)_rep1

description: sample 12-1-3


title: A549_Second_BatchD_ethanol extract of Bupleuri Radix (High dose)_rep2

description: sample 13-2-1


title: A549_Second_BatchD_ethanol extract of Citri Unshius Pericarpium (Medium dose)_rep1

description: sample 12-1-2


title: A549_Second_BatchD_ethanol extract of Citri Unshius Pericarpium (High dose)_rep1

description: sample 12-1-1


title: A549_Second_BatchD_ethanol extract of Zizyphi Fructus (Low dose)_rep3

description: sample 11-3-6

value: Zizyphi Fructus ethanol extract 20 μg/mL


title: A549_Second_BatchD_ethanol extract of Zizyphi Fructus (Medium dose)_rep3

description: sample 11-3-5

value: Zizyphi Fructus ethanol extract 100 μg/mL


title: A549_Second_BatchD_ethanol extract of Coptidis Rhizoma (Medium dose)_rep3

description: sample 11-3-2

value: Coptidis Rhizoma ethanol extract 22 μg/mL


title: A549_Second_BatchD_ethanol extract of Cimicifugae Rhizoma (Medium dose)_rep3

description: sample 12-3-5


title: A549_Second_BatchD_ethanol extract of Zizyphi Fructus (High dose)_rep3

description: sample 11-3-4

value: Zizyphi Fructus ethanol extract 500 μg/mL


title: A549_Second_BatchD_ethanol extract of Coptidis Rhizoma (Low dose)_rep3

description: sample 11-3-3

value: Coptidis Rhizoma ethanol extract 4.4 μg/mL


title: A549_Second_BatchD_ethanol extract of Coptidis Rhizoma (High dose)_rep3

description: sample 11-3-1

value: Coptidis Rhizoma ethanol extract 110 μg/mL


title: A549_Second_BatchD_ethanol extract of Zizyphi Fructus (Medium dose)_rep2

description: sample 11-2-5


title: A549_Second_BatchD_ethanol extract of Zizyphi Fructus (Low dose)_rep2

description: sample 11-2-6


title: A549_Second_BatchD_ethanol extract of Zizyphi Fructus (High dose)_rep2

description: sample 11-2-4


title: A549_Second_BatchD_ethanol extract of Coptidis Rhizoma (Medium dose)_rep2

description: sample 11-2-2


title: A549_Second_BatchD_ethanol extract of Coptidis Rhizoma (Low dose)_rep2

description: sample 11-2-3


title: A549_Second_BatchD_ethanol extract of Coptidis Rhizoma (High dose)_rep2

description: sample 11-2-1


title: A549_Second_BatchD_Vehicle(DMSO)_rep3

description: sample D1-3-1

value: 0.1% DMSO


title: A549_Second_BatchD_Vehicle(DMSO)_rep2

description: sample D1-2-1


title: A549_Second_BatchD_Vehicle(DMSO)_rep1

description: sample D1-1-1


title: A549_Second_BatchC_ethanol extract of Coptidis Rhizoma (Low dose)_rep1

description: sample 11-1-3


title: A549_Second_BatchC_ethanol extract of Coptidis Rhizoma (Medium dose)_rep1

description: sample 11-1-2


title: A549_Second_BatchC_ethanol extract of Zizyphi Fructus (Low dose)_rep1

description: sample 11-1-6


title: A549_Second_BatchC_ethanol extract of Zizyphi Fructus (High dose)_rep1

description: sample 11-1-4


title: A549_Second_BatchC_ethanol extract of Zizyphi Fructus (Medium dose)_rep1

description: sample 11-1-5


title: A549_Second_BatchC_ethanol extract of Scutellariae Radix (Low dose)_rep3

description: sample 10-3-6

value: Scutellariae Radix ethanol extract 7.6 μg/mL


title: A549_Second_BatchC_ethanol extract of Scutellariae Radix (Medium dose)_rep3

description: sample 10-3-5

value: Scutellariae Radix ethanol extract 38 μg/mL


title: A549_Second_BatchC_ethanol extract of Coptidis Rhizoma (High dose)_rep1

description: sample 11-1-1


title: A549_Second_BatchC_ethanol extract of Scutellariae Radix (Low dose)_rep2

description: sample 10-2-6


title: A549_Second_BatchC_ethanol extract of Scutellariae Radix (High dose)_rep3

description: sample 10-3-4

value: Scutellariae Radix ethanol extract 190 μg/mL


title: A549_Second_BatchC_ethanol extract of Pinelliae Tuber (High dose)_rep3

description: sample 10-3-1

value: Pinelliae Tuber ethanol extract 500 μg/mL


title: A549_Second_BatchC_ethanol extract of Scutellariae Radix (Medium dose)_rep2

description: sample 10-2-5


title: A549_Second_BatchC_ethanol extract of Pinelliae Tuber (Medium dose)_rep3

description: sample 10-3-2

value: Pinelliae Tuber ethanol extract 100 μg/mL


title: A549_Second_BatchC_ethanol extract of Scutellariae Radix (High dose)_rep2

description: sample 10-2-4


title: A549_Second_BatchC_ethanol extract of Pinelliae Tuber (Low dose)_rep3

description: sample 10-3-3

value: Pinelliae Tuber ethanol extract 20 μg/mL


title: A549_Second_BatchC_ethanol extract of Pinelliae Tuber (Low dose)_rep2

description: sample 10-2-3


title: A549_Second_BatchC_ethanol extract of Pinelliae Tuber (Medium dose)_rep2

description: sample 10-2-2


title: A549_Second_BatchC_ethanol extract of Scutellariae Radix (Low dose)_rep1

description: sample 10-1-6


title: A549_Second_BatchC_ethanol extract of Scutellariae Radix (High dose)_rep1

description: sample 10-1-4


title: A549_Second_BatchC_ethanol extract of Pinelliae Tuber (High dose)_rep2

description: sample 10-2-1


title: A549_Second_BatchC_ethanol extract of Scutellariae Radix (Medium dose)_rep1

description: sample 10-1-5


title: A549_Second_BatchC_ethanol extract of Pinelliae Tuber (Low dose)_rep1

description: sample 10-1-3


title: A549_Second_BatchC_ethanol extract of Pinelliae Tuber (Medium dose)_rep1

description: sample 10-1-2


title: A549_Second_BatchC_ethanol extract of Pinelliae Tuber (High dose)_rep1

description: sample 10-1-1


title: A549_Second_BatchC_ethanol extract of Zingiberis Rhizoma Recens (Low dose)_rep3

description: sample 9-3-3

value: Zingiberis Rhizoma Recens ethanol extract 20 μg/mL


title: A549_Second_BatchC_ethanol extract of Zingiberis Rhizoma (High dose)_rep3

description: sample 9-3-4

value: Zingiberis Rhizoma ethanol extract 500 μg/mL


title: A549_Second_BatchC_ethanol extract of Zingiberis Rhizoma (Medium dose)_rep3

description: sample 9-3-5

value: Zingiberis Rhizoma ethanol extract 100 μg/mL


title: A549_Second_BatchC_ethanol extract of Zingiberis Rhizoma Recens (High dose)_rep3

description: sample 9-3-1

value: Zingiberis Rhizoma Recens ethanol extract 500 μg/mL


title: A549_Second_BatchC_ethanol extract of Zingiberis Rhizoma Recens (Medium dose)_rep3

description: sample 9-3-2

value: Zingiberis Rhizoma Recens ethanol extract 100 μg/mL


title: A549_Second_BatchC_ethanol extract of Zingiberis Rhizoma (Low dose)_rep2

description: sample 9-2-6

value: Zingiberis Rhizoma ethanol extract 20 μg/mL


title: A549_Second_BatchC_ethanol extract of Zingiberis Rhizoma (Medium dose)_rep2

description: sample 9-2-5


title: A549_Second_BatchC_ethanol extract of Zingiberis Rhizoma Recens (Low dose)_rep2

description: sample 9-2-3


title: A549_Second_BatchC_ethanol extract of Zingiberis Rhizoma (High dose)_rep2

description: sample 9-2-4


title: A549_Second_BatchC_ethanol extract of Zingiberis Rhizoma Recens (Medium dose)_rep2

description: sample 9-2-2


title: A549_Second_BatchC_ethanol extract of Zingiberis Rhizoma Recens (High dose)_rep2

description: sample 9-2-1


title: A549_Second_BatchC_ethanol extract of Zingiberis Rhizoma (Low dose)_rep1

description: sample 9-1-6


title: A549_Second_BatchC_ethanol extract of Zingiberis Rhizoma (Medium dose)_rep1

description: sample 9-1-5


title: A549_Second_BatchC_ethanol extract of Zingiberis Rhizoma (High dose)_rep1

description: sample 9-1-4


title: A549_Second_BatchC_ethanol extract of Zingiberis Rhizoma Recens (High dose)_rep1

description: sample 9-1-1


title: A549_Second_BatchC_ethanol extract of Zingiberis Rhizoma Recens (Medium dose)_rep1

description: sample 9-1-2


title: A549_Second_BatchC_ethanol extract of Zingiberis Rhizoma Recens (Low dose)_rep1

description: sample 9-1-3


title: A549_Second_BatchC_water extract of Bojungikgi-tang (Medium dose)_rep3

description: sample 8-3-2

value: Bojungikgi-tang water extract 100 μg/mL


title: A549_Second_BatchC_water extract of Sayeok-tang (Low dose)_rep3

description: sample 8-3-6

value: Sayeok-tang water extract 20 μg/mL


title: A549_Second_BatchC_water extract of Sayeok-tang (Medium dose)_rep3

description: sample 8-3-5

value: Sayeok-tang water extract 100 μg/mL


title: A549_Second_BatchC_ethanol extract of Zingiberis Rhizoma (Low dose)_rep3

description: sample 9-3-6


title: A549_Second_BatchC_water extract of Sayeok-tang (Low dose)_rep2

description: sample 8-2-6


title: A549_Second_BatchC_water extract of Bojungikgi-tang (High dose)_rep3

description: sample 8-3-1

value: Bojungikgi-tang water extract 500 μg/mL


title: A549_Second_BatchC_water extract of Bojungikgi-tang (Low dose)_rep2

description: sample 8-2-3

value: Bojungikgi-tang water extract 20 μg/mL


title: A549_Second_BatchC_water extract of Sayeok-tang (Low dose)_rep1

description: sample 8-1-6


title: A549_Second_BatchC_water extract of Sayeok-tang (High dose)_rep2

description: sample 8-2-4

value: Sayeok-tang water extract 500 μg/mL


title: A549_Second_BatchC_water extract of Sayeok-tang (Medium dose)_rep2

description: sample 8-2-5


title: A549_Second_BatchC_Vehicle(DMSO)_rep2

description: sample C1-2-1

value: 0.1% DMSO


title: A549_Second_BatchC_water extract of Bojungikgi-tang (High dose)_rep2

description: sample 8-2-1


title: A549_Second_BatchC_water extract of Bojungikgi-tang (Medium dose)_rep2

description: sample 8-2-2


title: A549_Second_BatchC_water extract of Bojungikgi-tang (Low dose)_rep3

description: sample 8-3-3


title: A549_Second_BatchC_water extract of Sayeok-tang (Medium dose)_rep1

description: sample 8-1-5


title: A549_Second_BatchC_water extract of Sayeok-tang (High dose)_rep1

description: sample 8-1-4


title: A549_Second_BatchC_Vehicle(DMSO)_rep1

description: sample C1-1-1


title: A549_Second_BatchB_water extract of Banhasasim-tang (High dose)_rep3

description: sample 7-3-4

value: Banhasasim-tang water extract 500 μg/mL


title: A549_Second_BatchB_water extract of Bojungikgi-tang (Low dose)_rep1

description: sample 8-1-3


title: A549_Second_BatchB_water extract of Bojungikgi-tang (Medium dose)_rep1

description: sample 8-1-2


title: A549_Second_BatchC_Vehicle(DMSO)_rep3

description: sample C1-3-1


title: A549_Second_BatchB_water extract of Bojungikgi-tang (High dose)_rep1

description: sample 8-1-1


title: A549_Second_BatchC_water extract of Sayeok-tang (High dose)_rep3

description: sample 8-3-4


title: A549_Second_BatchB_water extract of Banhasasim-tang (Low dose)_rep3

description: sample 7-3-6

value: Banhasasim-tang water extract 20 μg/mL


title: A549_Second_BatchB_water extract of Yijung-tang (Low dose)_rep3

description: sample 7-3-3

value: Yijung-tang water extract 20 μg/mL


title: A549_Second_BatchB_water extract of Banhasasim-tang (Medium dose)_rep3

description: sample 7-3-5

value: Banhasasim-tang water extract 100 μg/mL


title: A549_Second_BatchB_water extract of Yijung-tang (Low dose)_rep2

description: sample 7-2-3


title: A549_Second_BatchB_water extract of Banhasasim-tang (High dose)_rep2

description: sample 7-2-4


title: A549_Second_BatchB_water extract of Banhasasim-tang (Low dose)_rep2

description: sample 7-2-6


title: A549_Second_BatchB_water extract of Yijung-tang (High dose)_rep3

description: sample 7-3-1

value: Yijung-tang water extract 500 μg/mL


title: A549_Second_BatchB_water extract of Banhasasim-tang (Medium dose)_rep2

description: sample 7-2-5


title: A549_Second_BatchB_water extract of Yijung-tang (High dose)_rep2

description: sample 7-2-1


title: A549_Second_BatchB_water extract of Yijung-tang (Medium dose)_rep2

description: sample 7-2-2

value: Yijung-tang water extract 100 μg/mL


title: A549_Second_BatchB_water extract of Banhasasim-tang (Low dose)_rep1

description: sample 7-1-6


title: A549_Second_BatchB_water extract of Banhasasim-tang (High dose)_rep1

description: sample 7-1-4


title: A549_Second_BatchB_water extract of Banhasasim-tang (Medium dose)_rep1

description: sample 7-1-5


title: A549_Second_BatchB_water extract of Yijung-tang (Medium dose)_rep1

description: sample 7-1-2


title: A549_Second_BatchB_water extract of Yijung-tang (High dose)_rep1

description: sample 7-1-1


title: A549_Second_BatchB_water extract of Aconiti Lateralis Radix Preparata (Low dose)_rep3

description: sample 6-3-6

value: Aconiti Lateralis Radix Preparata water extract 14.4 μg/mL


title: A549_Second_BatchB_water extract of Aconiti Lateralis Radix Preparata (Medium dose)_rep3

description: sample 6-3-5

value: Aconiti Lateralis Radix Preparata water extract 72 μg/mL


title: A549_Second_BatchB_water extract of Aconiti Lateralis Radix Preparata (High dose)_rep3

description: sample 6-3-4

value: Aconiti Lateralis Radix Preparata water extract 360 μg/mL


title: A549_Second_BatchB_water extract of Bupleuri Radix (Low dose)_rep3

description: sample 6-3-3

value: Bupleuri Radix water extract 20 μg/mL


title: A549_Second_BatchB_water extract of Bupleuri Radix (Medium dose)_rep3

description: sample 6-3-2

value: Bupleuri Radix water extract 100 μg/mL


title: A549_Second_BatchB_water extract of Bupleuri Radix (High dose)_rep3

description: sample 6-3-1

value: Bupleuri Radix water extract 500 μg/mL


title: A549_Second_BatchB_water extract of Aconiti Lateralis Radix Preparata (High dose)_rep2

description: sample 6-2-4


title: A549_Second_BatchB_water extract of Yijung-tang (Medium dose)_rep3

description: sample 7-3-2


title: A549_Second_BatchB_water extract of Aconiti Lateralis Radix Preparata (Low dose)_rep2

description: sample 6-2-6


title: A549_Second_BatchB_water extract of Aconiti Lateralis Radix Preparata (Medium dose)_rep2

description: sample 6-2-5


title: A549_Second_BatchB_water extract of Aconiti Lateralis Radix Preparata (Medium dose)_rep1

description: sample 6-1-5


title: A549_Second_BatchB_water extract of Bupleuri Radix (Medium dose)_rep2

description: sample 6-2-2


title: A549_Second_BatchB_water extract of Aconiti Lateralis Radix Preparata (High dose)_rep1

description: sample 6-1-4


title: A549_Second_BatchB_water extract of Bupleuri Radix (High dose)_rep2

description: sample 6-2-1


title: A549_Second_BatchB_water extract of Bupleuri Radix (Low dose)_rep1

description: sample 6-1-3


title: A549_Second_BatchB_water extract of Bupleuri Radix (High dose)_rep1

description: sample 6-1-1


title: A549_Second_BatchB_water extract of Aconiti Lateralis Radix Preparata (Low dose)_rep1

description: sample 6-1-6


title: A549_Second_BatchB_water extract of Bupleuri Radix (Medium dose)_rep1

description: sample 6-1-2


title: A549_Second_BatchB_water extract of  Cimicifugae Rhizoma (Low dose)_rep3

description: sample 5-3-6

value: Cimicifugae Rhizoma water extract 20 μg/mL


title: A549_Second_BatchB_water extract of Yijung-tang (Low dose)_rep1

description: sample 7-1-3


title: A549_Second_BatchB_water extract of  Cimicifugae Rhizoma (High dose)_rep3

description: sample 5-3-4

value: Cimicifugae Rhizoma water extract 500 μg/mL


title: A549_Second_BatchB_water extract of  Cimicifugae Rhizoma (Medium dose)_rep3

description: sample 5-3-5

value: Cimicifugae Rhizoma water extract 100 μg/mL


title: A549_Second_BatchB_water extract of Citri Unshius Pericarpium (Low dose)_rep3

description: sample 5-3-3

value: Citri Unshius Pericarpium water extract 20 μg/mL


title: A549_Second_BatchB_water extract of Citri Unshius Pericarpium (Medium dose)_rep3

description: sample 5-3-2

value: Citri Unshius Pericarpium water extract 100 μg/mL


title: A549_Second_BatchB_water extract of  Cimicifugae Rhizoma (Low dose)_rep2

description: sample 5-2-6


title: A549_Second_BatchB_water extract of Citri Unshius Pericarpium (Medium dose)_rep2

description: sample 5-2-2


title: A549_Second_BatchB_water extract of  Cimicifugae Rhizoma (Medium dose)_rep2

description: sample 5-2-5


title: A549_Second_BatchB_water extract of  Cimicifugae Rhizoma (High dose)_rep2

description: sample 5-2-4


title: A549_Second_BatchB_water extract of Citri Unshius Pericarpium (Low dose)_rep2

description: sample 5-2-3


title: A549_Second_BatchB_water extract of Citri Unshius Pericarpium (High dose)_rep3

description: sample 5-3-1

value: Citri Unshius Pericarpium water extract 500 μg/mL


title: A549_Second_BatchB_water extract of  Cimicifugae Rhizoma (Low dose)_rep1

description: sample 5-1-6


title: A549_Second_BatchB_water extract of Bupleuri Radix (Low dose)_rep2

description: sample 6-2-3


title: A549_Second_BatchB_water extract of  Cimicifugae Rhizoma (Medium dose)_rep1

description: sample 5-1-5


title: A549_Second_BatchB_water extract of Citri Unshius Pericarpium (Low dose)_rep1

description: sample 5-1-3


title: A549_Second_BatchB_water extract of Citri Unshius Pericarpium (High dose)_rep1

description: sample 5-1-1


title: A549_Second_BatchB_Vehicle(DMSO)_rep2

description: sample B1-2-1

value: 0.1% DMSO


title: A549_Second_BatchB_water extract of Citri Unshius Pericarpium (Medium dose)_rep1

description: sample 5-1-2


title: A549_Second_BatchA_water extract of Zizyphi Fructus (Medium dose)_rep3

description: sample 4-3-5

value: Zizyphi Fructus water extract 100 μg/mL


title: A549_Second_BatchA_water extract of Zizyphi Fructus (Low dose)_rep3

description: sample 4-3-6

value: Zizyphi Fructus water extract 20 μg/mL


title: A549_Second_BatchA_water extract of Zizyphi Fructus (High dose)_rep3

description: sample 4-3-4

value: Zizyphi Fructus water extract 500 μg/mL


title: A549_Second_BatchA_water extract of Coptidis Rhizoma (Medium dose)_rep3

description: sample 4-3-2

value: Coptidis Rhizoma water extract 26 μg/mL


title: A549_Second_BatchB_Vehicle(DMSO)_rep3

description: sample B1-3-1


title: A549_Second_BatchA_water extract of Coptidis Rhizoma (Low dose)_rep3

description: sample 4-3-3

value: Coptidis Rhizoma water extract 5.2 μg/mL


title: A549_Second_BatchB_water extract of  Cimicifugae Rhizoma (High dose)_rep1

description: sample 5-1-4


title: A549_Second_BatchA_water extract of Coptidis Rhizoma (High dose)_rep3

description: sample 4-3-1

value: Coptidis Rhizoma water extract 130 μg/mL


title: A549_Second_BatchA_water extract of Zizyphi Fructus (High dose)_rep2

description: sample 4-2-4


title: A549_Second_BatchA_water extract of Zizyphi Fructus (Medium dose)_rep2

description: sample 4-2-5


title: A549_Second_BatchA_water extract of Zizyphi Fructus (Low dose)_rep2

description: sample 4-2-6


title: A549_Second_BatchA_water extract of Coptidis Rhizoma (High dose)_rep2

description: sample 4-2-1


title: A549_Second_BatchA_water extract of Coptidis Rhizoma (Low dose)_rep2

description: sample 4-2-3


title: A549_Second_BatchA_water extract of Zizyphi Fructus (Low dose)_rep1

description: sample 4-1-6


title: A549_Second_BatchA_water extract of Coptidis Rhizoma (Medium dose)_rep2

description: sample 4-2-2


title: A549_Second_BatchB_water extract of Citri Unshius Pericarpium (High dose)_rep2

description: sample 5-2-1


title: A549_Second_BatchA_water extract of Coptidis Rhizoma (Medium dose)_rep1

description: sample 4-1-2


title: A549_Second_BatchA_water extract of Zizyphi Fructus (Medium dose)_rep1

description: sample 4-1-5


title: A549_Second_BatchA_water extract of Coptidis Rhizoma (Low dose)_rep1

description: sample 4-1-3


title: A549_Second_BatchA_water extract of Scutellariae Radix (Low dose)_rep3

description: sample 3-3-6

value: Scutellariae Radix water extract 9.2 μg/mL


title: A549_Second_BatchA_water extract of Scutellariae Radix (Medium dose)_rep3

description: sample 3-3-5

value: Scutellariae Radix water extract 46 μg/mL


title: A549_Second_BatchA_water extract of Pinelliae Tuber (Low dose)_rep3

description: sample 3-3-3

value: Pinelliae Tuber water extract 20 μg/mL


title: A549_Second_BatchA_water extract of Zizyphi Fructus (High dose)_rep1

description: sample 4-1-4


title: A549_Second_BatchA_water extract of Coptidis Rhizoma (High dose)_rep1

description: sample 4-1-1


title: A549_Second_BatchA_water extract of Pinelliae Tuber (Medium dose)_rep3

description: sample 3-3-2

value: Pinelliae Tuber water extract 100 μg/mL


title: A549_Second_BatchA_water extract of Scutellariae Radix (High dose)_rep3

description: sample 3-3-4

value: Scutellariae Radix water extract 230 μg/mL


title: A549_Second_BatchB_Vehicle(DMSO)_rep1

description: sample B1-1-1


title: A549_Second_BatchA_water extract of Pinelliae Tuber (High dose)_rep3

description: sample 3-3-1

value: Pinelliae Tuber water extract 500 μg/mL


title: A549_Second_BatchA_water extract of Scutellariae Radix (Low dose)_rep2

description: sample 3-2-6


title: A549_Second_BatchA_water extract of Scutellariae Radix (High dose)_rep2

description: sample 3-2-4


title: A549_Second_BatchA_water extract of Scutellariae Radix (Medium dose)_rep2

description: sample 3-2-5


title: A549_Second_BatchA_water extract of Pinelliae Tuber (Low dose)_rep2

description: sample 3-2-3


title: A549_Second_BatchA_water extract of Pinelliae Tuber (Medium dose)_rep2

description: sample 3-2-2


title: A549_Second_BatchA_water extract of Pinelliae Tuber (High dose)_rep2

description: sample 3-2-1


title: A549_Second_BatchA_water extract of Scutellariae Radix (Low dose)_rep1

description: sample 3-1-6


title: A549_Second_BatchA_water extract of Scutellariae Radix (High dose)_rep1

description: sample 3-1-4


title: A549_Second_BatchA_water extract of Pinelliae Tuber (Low dose)_rep1

description: sample 3-1-3


title: A549_Second_BatchA_water extract of Pinelliae Tuber (High dose)_rep1

description: sample 3-1-1


title: A549_Second_BatchA_water extract of Scutellariae Radix (Medium dose)_rep1

description: sample 3-1-5


title: A549_Second_BatchA_water extract of Zingiberis Rhizoma (High dose)_rep3

description: sample 2-3-4

value: Zingiberis Rhizoma water extract 500 μg/mL


title: A549_Second_BatchA_water extract of Pinelliae Tuber (Medium dose)_rep1

description: sample 3-1-2


title: A549_Second_BatchA_water extract of Zingiberis Rhizoma (Low dose)_rep3

description: sample 2-3-6

value: Zingiberis Rhizoma water extract 20 μg/mL


title: A549_Second_BatchA_water extract of Zingiberis Rhizoma (Medium dose)_rep3

description: sample 2-3-5

value: Zingiberis Rhizoma water extract 100 μg/mL


title: A549_Second_BatchA_water extract of Zingiberis Rhizoma Recens (Low dose)_rep3

description: sample 2-3-3

value: Zingiberis Rhizoma Recens water extract 20 μg/mL


title: A549_Second_BatchA_water extract of Zingiberis Rhizoma Recens (Medium dose)_rep3

description: sample 2-3-2

value: Zingiberis Rhizoma Recens water extract 100 μg/mL


title: A549_Second_BatchA_water extract of Zingiberis Rhizoma Recens (High dose)_rep3

description: sample 2-3-1

value: Zingiberis Rhizoma Recens water extract 500 μg/mL


title: A549_Second_BatchA_water extract of Zingiberis Rhizoma (Medium dose)_rep2

description: sample 2-2-5


title: A549_Second_BatchA_water extract of Zingiberis Rhizoma Recens (High dose)_rep2

description: sample 2-2-1


title: A549_Second_BatchA_water extract of Zingiberis Rhizoma (Low dose)_rep2

description: sample 2-2-6


title: A549_Second_BatchA_water extract of Zingiberis Rhizoma (Low dose)_rep1

description: sample 2-1-6


title: A549_Second_BatchA_water extract of Zingiberis Rhizoma Recens (Medium dose)_rep2

description: sample 2-2-2


title: A549_Second_BatchA_water extract of Zingiberis Rhizoma (High dose)_rep2

description: sample 2-2-4


title: A549_Second_BatchA_water extract of Zingiberis Rhizoma Recens (Low dose)_rep2

description: sample 2-2-3


title: A549_Second_BatchA_water extract of Zingiberis Rhizoma (High dose)_rep1

description: sample 2-1-4


title: A549_Second_BatchA_water extract of Zingiberis Rhizoma (Medium dose)_rep1

description: sample 2-1-5


title: human high-grade ovarian carcinoma sample 3

city: Halle

first: Markus

last: Glaß

address: Kurt-Mothes-Str. 3a

department: Institute of Molecular Medicine

country: Germany

institute: Martin Luther University Halle-Wittenberg

description:
Library name: HGSC3
BD Rhapsody HT

library_source: transcriptomic single cell

data_processing:
Read processing including demultiplexing, genome mapping and gene counting was performed using the BD Rhapsody Sequence Analysis Pipeline v2.0 at www.sevenbridges.com
Assembly: GRCh38
Supplementary files format and content: 10x compatible tab separated value files and matrix files

source_name: dissociated tumor tissue

extract_protocol:
Tumors were minced and dissociated using the human tumor dissociation kit (Miltenyi Biotec), depleted for erytrocytes and filtered. Cells were cryopreserved until scRNA-seq was performed. If applicable, dead cells were removed using the dead cellremoval kit (Miltenyi Biotec).
Single cells were captured using the BD Rhapsody HT Express and the BD Rhapsody Scanner. cDNA synthesis and WTA library constructions was perfomed according to the manufacturer's instructions.

value: dissociated tumor tissue

value: tumor and immune cells

value: untreated


title: A549_Second_BatchA_water extract of Zingiberis Rhizoma Recens (Low dose)_rep1

description: sample 2-1-3


title: A549_Second_BatchA_water extract of Zingiberis Rhizoma Recens (Medium dose)_rep1

description: sample 2-1-2


title: A549_Second_BatchA_water extract of Zingiberis Rhizoma Recens (High dose)_rep1

description: sample 2-1-1


title: BTYNB-10pre_Killing_Tcells, Rep 2

city: Halle (Saale)

first: Danny

last: Misiak

department: Molecular Cell Biology

data_processing:
Sequenced strand unspecific paired-end reads were trimmed for adaptor sequences and low quality sequence using Cutadapt (v 2.8) with parameters --nextseq-trim=20 -m 20 --trim-n -u 12 -A N{12}
Trimmed reads were mapped against the human genome (hg38 UCSC) using HiSat2 (v 2.1.0) with parameters -q --rna-strandness FR --dta -k 5
Secondary alignments were filtered out using samtools (v 1.10).
Mapped reads were summarized using featureCounts (v 2.0.0) with parameterst -p -s 1 -T 6 -M -t exon -g gene_id and Ensembl gene annotations (GRCh 38.102)
Assembly: hg38
Supplementary files format and content: Comma-separated text files including raw counts for each sample.

source_name: T-cell

extract_protocol:
Poly(A) RNA enrichment was performed with the poly(A) RNA Selection kit V1.5 (Lexogen). The CORALL Total RNA-Seq V1 (Lexogen) was used for strand-specific library generation. Library preparation and sequencing of paired-end reads was performed on an Illumina NextSeq 1000 platform at the Core Facility Imaging (MLU Halle).
Lexogen CORALL RNA-Seq with UDIs

treatment_protocol: ES-2 cells were treated with 10 µM BTYNB 24h before HLA-matched human PBMCs in a 1:2 ratio together with CD3/CD28 activation beads (Thermo Fisher) were added for additional 36h. ES-2 cells and PBMCs were separated by FACS sorting before RNA was isolated by TRIZOL. RNA integrity was examined using a BioAnalyzer 2100 (Agilent).

value: T-cell

value: ovarian cancer derived cell line

value: BTYNB treatment

value: 10uM BTYNB


title: human high-grade ovarian carcinoma sample 1

description:
Library name: HGSC1
BD Rhapsody HT


title: A549_Second_BatchA_Wortmannin_rep3

description: sample 1-3-4

value: wortmannin 10 uM


title: DMSO_Killing_Tcells, Rep 2

value: WT


title: A549_Second_BatchA_Wortmannin_rep1

description: sample 1-1-4


title: DMSO_Killing_Tcells, Rep 1


title: BTYNB-10pre_Killing_ES2, Rep 2


title: A549_Second_BatchA_Vehicle(DMSO)_rep3

description: sample A1-3-1

value: 0.1% DMSO


title: A549_Second_BatchA_Vehicle(DMSO)_rep1

description: sample A1-1-1


title: A549_Second_BatchA_Vehicle(DMSO)_rep2

description: sample A1-2-1


title: BTYNB-10pre_Killing_ES2, Rep 1


title: DMSO, Killing, ES2, Rep 2


title: BatchE_ethanol extract of Sayeok-tang (Low dose)_rep3

description: sample 15-3-6

growth_protocol: Human colorectal adenocarcinoma cell line, HT29 (HTB-38) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). HT29 cells were maintained in DMEM medium supplemented with 10% (v/v) heat-inactivated FBS, 100 IU/mL penicillin, and 100 mg/mL streptomycin at 37 °C, 5% CO2 incubator. The cell was subcultured every 3 or 4 days, depending on the cell density.

treatment_protocol: Dulbecco's Modified Eagle Medium (DMEM), phosphate-buffered saline (PBS), TrypLE Express, penicillin-streptomycin, and fetal bovine serum (FBS) were purchased from Gibco (Grand Island, NY, USA). Cell culture flasks and multiwell culture plates were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Dimethyl sulfoxide (DMSO) and QIAzol lysis reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA) and Qiagen (Germantown, MD, USA), respectively. The dried medicinal plants, prepared in compliance with the Korean Pharmacopoeia, were supplied from Kwangmyung-dang Medicinal Herbs Co. (Ulsan, Republic of Korea). The samples used in the experiment underwent organoleptic examination by Dr. Choi Goya, an herbal medicine organoleptic examination expert appointed by the Korea Food and Drug Administration, and were additionally identified at the species level by analyzing the DNA barcode region sequences. Voucher specimen were deposited in the Korean Herbarium of Standard Herbal Resources, Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine, Naju, Republic of Korea . All herbs and extracts can be obtained from the Oriental Medicine Resources Research Center (KIOM, https://oasis.kiom.re.kr/herblib). A hot water and 70% ethanol extract of each plant was prepared and supplied by KOC Biotech Co. (Daejeon, Republic of Korea). Briefly, dried plants (1,000 g) were pulverized and extracted in hot distilled water (10 L) for 3 h under a reflux extraction system (MS-DM609; MTOPS, Seoul, Republic of Korea) or in 70% ethanol (15L) for 1 h under ultrasonication system (VCP-20, Lab companion, Dajeon, Republic of Korea) twice. The extract solution was filtered through a 5 µm cartridge filter, concentrated in a rotary evaporator (Ev-1020, SciLab, Seoul, Republic of Korea), and then lyophilized in a freeze dryer (LP-20, Ilshin-Bio-Base, Dongduchen, Republic of Korea) to obtain the final extract. The extract was finely homogenized and packed in a glass bottle containing a desiccant silica gel. The decoction was prepared by mixing each extract, considering the composition ratio and extract yield of a single medicinal herb, and homogenized, according to the Korean Pharmacopoeia. For the in vitro treatment, extracts (100 mg) were vigorously vortexed for 30 min in 10 mL phosphate-buffered saline (PBS; Thermo Fisher Scientific, Rockford, IL, USA) containing 2% DMSO and then sterilized by filtering through a 0.22 µm membrane to obtain a stock solution (10 mg/mL); the stock solution was aliquoted in small volumes and stored at -80°C until use.

value: Sayeok-tang ethanol extract 20 μg/mL


title: BatchE_ethanol extract of Sayeok-tang (Medium dose)_rep3

description: sample 15-3-5

value: Sayeok-tang ethanol extract 100 μg/mL


title: BatchE_ethanol extract of Sayeok-tang (High dose)_rep3

description: sample 15-3-4

value: Sayeok-tang ethanol extract 500 μg/mL


title: ChIP-seq_pY1_CH6953755_1uM_rep2

description:
Library name: sample225
ChIP-seq_pY1_CH6953755_1uM_merge.bw

data_processing:
ChIP-seq raw data were processed using a custom pipeline that builds on published workflows with minor modifications. Briefly, reads were firstly trimmed for adapter sequences with trim_galore (v.0.6.2).
Then trimmed reads were mapped to the concatenated index of human reference genome (hg38) and mouse reference genome (mm10) using Bowtie2 (v.2.2.3).
The final bam files were converted to bigwig file using bamCoverage method.
Assembly: Concatenated index of hg38 and mm10
Supplementary files format and content: bigwig

extract_protocol:
Sample preparation were similar as standard ChIP-seq protocols with some modifications. Briefly, cells were fixed with 1% formalaldehyde, and chromatin fractions were seperated. After sonication, free chromatin fractions were incubated with corresponding antibodies overnight at 4°C with rotations. Antibody-bound proteins were captured with potein G beads, and after washing, protein-bound genomic DNA was extracted and purified.
Library construction was performed following the VAHTS Universal Pro DNA Library Prep Kit for Illumina (Vazyme #ND608) instructions.

treatment_protocol: TG003 (1 μM) treatment for 4 hours; CH6953755 (1 μM) treatment for 24 hours.

value: CH6953755 (1 μM) for 24 hours


title: ChIP-seq_pY1_CH6953755_0uM_rep2

description:
Library name: sample223
ChIP-seq_pY1_CH6953755_0uM_merge.bw


title: BatchE_ethanol extract of Bojungikgi-tang (High dose)_rep3

description: sample 15-3-1

value: Bojungikgi-tang ethanol extract 500 μg/mL


title: ChIP-seq_pS7_TG003_1uM_rep2

description:
Library name: sample221
ChIP-seq_pS7_TG003_1uM_merge.bw

value: TG003 (1 μM) for 4 hours


title: ChIP-seq_pY1_CH6953755_0uM_rep1

description:
Library name: sample222
ChIP-seq_pY1_CH6953755_0uM_merge.bw


title: BTYNB-10pre_Killing_Tcells, Rep 1


title: ChIP-seq_pS7_TG003_1uM_rep1

description:
Library name: sample220
ChIP-seq_pS7_TG003_1uM_merge.bw


title: BatchE_ethanol extract of Sayeok-tang (Medium dose)_rep2

description: sample 15-2-5


title: ChIP-seq_pS7_TG003_0uM_rep1

description:
Library name: sample218
ChIP-seq_pS7_TG003_0uM_merge.bw


title: BatchE_ethanol extract of Sayeok-tang (Low dose)_rep2

description: sample 15-2-6


title: A549_Second_BatchA_Wortmannin_rep2

description: sample 1-2-4


title: ChIP-seq_YES1_KO_rep2

description:
Library name: sample217
ChIP-seq_YES1_YES1_KO_merge.bw

value: YES1 K.O.


title: BatchE_ethanol extract of Bojungikgi-tang (High dose)_rep2

description: sample 15-2-1


title: ChIP-seq_CLK4_KO_rep2

description:
Library name: sample215
ChIP-seq_CLK4_CLK4_KO_merge.bw

value: CLK4 K.O.


title: ChIP-seq_YES1_KO_rep1

description:
Library name: sample216
ChIP-seq_YES1_YES1_KO_merge.bw


title: BatchE_ethanol extract of Bojungikgi-tang (Medium dose)_rep2

description: sample 15-2-2

value: Bojungikgi-tang ethanol extract 100 μg/mL


title: DMSO, Killing, ES2, Rep 1

value: untreated


title: BatchE_ethanol extract of Bojungikgi-tang (Low dose)_rep2

description: sample 15-2-3

value: Bojungikgi-tang ethanol extract 20 μg/mL


title: ChIP-seq_CLK4_KO_rep1

description:
Library name: sample214
ChIP-seq_CLK4_CLK4_KO_merge.bw


title: BatchE_ethanol extract of Sayeok-tang (High dose)_rep1

description: sample 15-1-4


title: BatchE_ethanol extract of Sayeok-tang (Medium dose)_rep1

description: sample 15-1-5


title: ChIP-seq_CLK1_KO_rep2

description:
Library name: sample213
ChIP-seq_CLK1_CLK1_KO_merge.bw

value: CLK1 K.O.


title: ChIP-seq_pY1_CH6953755_1uM_rep1

description:
Library name: sample224
ChIP-seq_pY1_CH6953755_1uM_merge.bw


title: BatchE_ethanol extract of Bojungikgi-tang (Medium dose)_rep3

description: sample 15-3-2


title: ChIP-seq_CLK1_KO_rep1

description:
Library name: sample212
ChIP-seq_CLK1_CLK1_KO_merge.bw


title: BatchE_ethanol extract of Bojungikgi-tang (Low dose)_rep1

description: sample 15-1-3


title: ChIP-seq_pY1_YES1_KO_rep1

description:
Library name: sample210
ChIP-seq_pY1_YES1_KO_merge.bw

treatment_protocol: TG003 (20 μM) treatment for 4 hours; CH6953755 (10 μM) treatment for 24 hours.

value: YES1 knock-out


title: ChIP-seq_pY1_YES1_KO_rep2

description:
Library name: sample211
ChIP-seq_pY1_YES1_KO_merge.bw


title: BatchE_ethanol extract of Bojungikgi-tang (Low dose)_rep3

description: sample 15-3-3


title: BatchE_ethanol extract of Bojungikgi-tang (High dose)_rep1

description: sample 15-1-1


title: BatchE_ethanol extract of Sayeok-tang (High dose)_rep2

description: sample 15-2-4


title: BatchE_ethanol extract of Bojungikgi-tang (Medium dose)_rep1

description: sample 15-1-2


title: ChIP-seq_pY1_YES1_ctrl_rep2

description:
Library name: sample209
ChIP-seq_pY1_YES1_ctrl_merge.bw


title: BatchE_ethanol extract of Banhasasim-tang (Low dose)_rep3

description: sample 14-3-6

value: Banhasasim-tang ethanol extract 20 μg/mL


title: BatchE_ethanol extract of Banhasasim-tang (Medium dose)_rep3

description: sample 14-3-5

value: Banhasasim-tang ethanol extract 100 μg/mL


title: ChIP-seq_pS7_TG003_0uM_rep2

description:
Library name: sample219
ChIP-seq_pS7_TG003_0uM_merge.bw

value: WT


title: ChIP-seq_pY1_YES1_ctrl_rep1

description:
Library name: sample208
ChIP-seq_pY1_YES1_ctrl_merge.bw


title: BatchE_ethanol extract of Banhasasim-tang (High dose)_rep3

description: sample 14-3-4

value: Banhasasim-tang ethanol extract 500 μg/mL


title: ChIP-seq_RNAPII_YES1_KO_rep2

description:
Library name: sample207
ChIP-seq_RNAPII_YES1_KO_merge.bw


title: BatchE_ethanol extract of Sayeok-tang (Low dose)_rep1

description: sample 15-1-6

value: Sayeok-tang ethanol extract 20 μg/mL


title: ChIP-seq_RNAPII_YES1_KO_rep1

description:
Library name: sample206
ChIP-seq_RNAPII_YES1_KO_merge.bw