title: BSC, stimulated with hemoglobin-haptoglobin (400 µM) for 48 h, rep4

description: Library name: E1_65_48_Hpx400_F1


title: BSC, stimulated with hemoglobin (400 µM) for 48 h, rep9

status: Public on Dec 29 2025

type: SRA

city: Zürich

first: Raphael

middle: Matthias

last: Buzzi

address: Rämistrasse 100

department: Division of Internal Medicine

country: Switzerland

institute: Universitätsspital and University of Zurich

description: Library name: E1_63_48_Hb400_F1

library_source: transcriptomic

data_processing:
equence reads were processed using the FeatureCountsApp workflow. Reads were aligned to the Mus musculus GRCm39 reference genome in antisense strand mode, with a minimum mapping quality of 10, allowing multi-overlaps and counting multi-hits. FeatureCounts was run at the gene level with transcript type restricted to protein-coding, using paired-end or single-end mode as appropriate.
Assembly: GRCm39
Supplementary files format and content: Each processed data file contains normalized gene expression counts from RNA-seq experiments, with rows corresponding to gene identifiers and columns providing expression values for each gene in a given sample. The files are tab-delimited and are suitable for downstream analysis such as differential expression or pathway enrichment. All files use consistent formatting to facilitate direct comparison across experimental groups.

molecule: total RNA

organism: Mus musculus

source_name: brain

growth_protocol: Coronal BSCs were prepared essentially as described by Stoppini et al. (1991) with minor adaptations.(Stoppini et al. 1991; Michler-Stuke et al. 1984; Sünwoldt et al. 2017; Voss et al. 2020) Briefly, deeply anesthetized mice were transcardially perfused with ice-cold phosphate-buffered saline (PBS, Gibco 17502048). Brains were removed, glued to the vibratome stage, and sectioned coronally (150 μm; speed 4, frequency 10) into Hibernate‑A at 4°C (<15 min). Two slices were placed on each PTFE insert (0.4 μm, Millicell) in 6‑well plates containing 1.1 mL plating medium (Neurobasal‑A + 20 % heat‑inactivated horse serum + antibiotic‑antimycotic). Cultures were kept at 37 °C, 5 % CO₂. On day 4, medium was replaced with serum‑free growth medium (Neurobasal‑A supplemented with N‑2, B‑27 Plus, G‑5, and antibiotics). Experiments were performed on day 7 or, for longer cultivation, after a half‑medium change.

extract_protocol:
Lexogen SPLIT kit
Illumina Truseq mRNA

treatment_protocol: On day 7, slices were treated for 24–48 hours with 200 μM Hb or heme-albumin, with or without stoichiometric Hp or Hpx.

tag: tissue

value: brain

tag: cell type

value: brain slice cultur

tag: strain

tag: treatment

tag: batch


title: BSC, control (0 µM, 24 h), rep10

description: Library name: E1_6_24_ctrl_F3


title: BSC, control (0 µM, 48 h), rep8

description: Library name: E1_56_48_ctrl_F1


title: BSC, stimulated with hemoglobin-haptoglobin (400 µM) for 48 h, rep2

description: Library name: E1_55_48_Hpx400_F2


title: BSC, stimulated with hemoglobin-haptoglobin (400 µM) for 48 h, rep3

description: Library name: E1_60_48_Hpx400_F2


title: BSC, stimulated with haptoglobin (400 µM) for 48 h, rep3

description: Library name: E1_64_48_Hp400_F1


title: BSC, stimulated with hemoglobin-haptoglobin (400 µM) for 48 h

description: Library name: E1_50_48_Hpx400_F5


title: BSC, stimulated with haptoglobin (400 µM) for 24 h, rep6

description: Library name: E1_4_24_Hp400_F3


title: BSC, stimulated with haptoglobin (400 µM) for 48 h

description: Library name: E1_49_48_Hp400_F5


title: BSC, stimulated with hemoglobin-haptoglobin (400 µM) for 24 h, rep7

description: Library name: E1_45_24_Hpx400_M5


title: BSC, stimulated with hemoglobin (400 µM) for 24 h, rep10

description: Library name: E2_37_24_Hb400_F1


title: BSC, stimulated with haptoglobin (400 µM) for 24 h, rep5

description: Library name: E1_44_24_Hp400_M5


title: BSC, stimulated with haptoglobin (400 µM) for 24 h, rep4

description: Library name: E1_39_24_Hp400_M4


title: BSC, stimulated with hemoglobin-haptoglobin (400 µM) for 24 h, rep6

description: Library name: E1_40_24_Hpx400_M4


title: BSC, stimulated with hemoglobin (200 µM) for 48 h, rep9

description: Library name: E1_52_48_Hb200_F2


title: BSC, stimulated with hemoglobin (200 µM) for 24 h, rep10

description: Library name: E1_37_24_Hb200_M4


title: BSC, stimulated with hemoglobin (200 µM) for 48 h, rep10

description: Library name: E2_10_48_Hb200_M1


title: BSC, stimulated with haptoglobin (400 µM) for 24 h, rep3

description: Library name: E1_34_24_Hp400_M3


title: BSC, stimulated with hemoglobin-haptoglobin (400 µM) for 24 h, rep5

description: Library name: E1_35_24_Hpx400_M3


title: BSC, stimulated with hemoglobin-haptoglobin (400 µM) for 48 h, rep7

description: Library name: E1_80_48_Hpx400_M1


title: BSC, stimulated with hemoglobin (400 µM) for 24 h, rep9

description: Library name: E1_33_24_Hb400_M3


title: BSC, stimulated with hemoglobin-haptoglobin (400 µM) for 24 h, rep4

description: Library name: E1_30_24_Hpx400_M3


title: BSC, stimulated with hemoglobin-haptoglobin (400 µM) for 24 h, rep3

description: Library name: E1_25_24_Hpx400_F5


title: BSC, stimulated with hemoglobin-haptoglobin (400 µM) for 24 h, rep2

description: Library name: E1_15_24_Hpx400_F4


title: BSC, stimulated with haptoglobin (400 µM) for 24 h, rep2

description: Library name: E1_29_24_Hp400_M3


title: BSC, stimulated with hemoglobin (200 µM) for 24 h, rep9

description: Library name: E1_17_24_Hb200_F4


title: BSC, stimulated with haptoglobin (400 µM) for 48 h, rep2

description: Library name: E1_54_48_Hp400_F2


title: BSC, stimulated with haptoglobin (400 µM) for 24 h

description: Library name: E1_14_24_Hp400_F3


title: BSC, control (0 µM, 24 h), rep9

description: Library name: E2_6_48_ctrl_F1


title: BSC, stimulated with hemoglobin-haptoglobin (400 µM) for 24 h

description: Library name: E1_10_24_Hpx400_F3


title: BSC, stimulated with hemoglobin (400 µM) for 24 h, rep7

description: Library name: E2_35_24_Hb400_M4


title: BSC, stimulated with hemoglobin (400 µM) for 24 h, rep8

description: Library name: E2_39_24_Hb400_F2


title: BSC, control (0 µM, 24 h), rep8

description: Library name: E2_5_24_ctrl_M1


title: BSC, stimulated with hemoglobin (400 µM) for 48 h, rep8

description: Library name: E2_34_48_Hb400_F3


title: BSC, stimulated with hemoglobin (400 µM) for 24 h, rep6

description: Library name: E2_33_24_Hb400_M3


title: BSC, stimulated with hemoglobin (200 µM) for 24 h, rep8

description: Library name: E2_27_24_Hb200_M4


title: BSC, stimulated with hemoglobin (200 µM) for 48 h, rep8

description: Library name: E2_20_48_Hb200_F3


title: BSC, stimulated with hemoglobin (400 µM) for 48 h, rep7

description: Library name: E2_26_48_Hb400_M3


title: BSC, stimulated with hemoglobin (200 µM) for 24 h, rep7

description: Library name: E2_25_24_Hb200_M3


title: BSC, stimulated with hemoglobin (200 µM) for 48 h, rep7

description: Library name: E2_16_48_Hb200_F1


title: BSC, stimulated with hemoglobin-haptoglobin complex (200 µM) for 48 h, rep4

description: Library name: E2_42_48_HbHp200_M4


title: BSC, control (0 µM, 24 h), rep7

description: Library name: E2_15_24_ctrl_F4


title: BSC, control (0 µM, 48 h), rep6

description: Library name: E1_86_48_ctrl_M4


title: BSC, stimulated with hemoglobin (400 µM) for 48 h, rep6

description: Library name: E1_83_48_Hb400_M5


title: BSC, control (0 µM, 48 h), rep5

description: Library name: E1_81_48_ctrl_M5


title: BSC, stimulated with hemoglobin (200 µM) for 48 h, rep5

description: Library name: E1_82_48_Hb200_M5


title: BSC, stimulated with hemoglobin (200 µM) for 48 h, rep4

description: Library name: E1_77_48_Hb200_M1


title: BSC, stimulated with hemoglobin (400 µM) for 24 h, rep5

description: Library name: E1_8_24_Hb400_F3


title: BSC, stimulated with hemoglobin (400 µM) for 48 h, rep5

description: Library name: E1_78_48_Hb400_M1


title: BSC, control (0 µM, 48 h), rep7

description: Library name: E2_8_48_ctrl_F2


title: BSC, stimulated with hemoglobin (200 µM) for 48 h, rep3

description: Library name: E1_67_48_Hb200_M2


title: BSC, stimulated with hemoglobin (200 µM) for 48 h, rep2

description: Library name: E1_62_48_Hb200_F1


title: BSC, control (0 µM, 48 h), rep3

description: Library name: E1_66_48_ctrl_M2


title: BSC, stimulated with hemoglobin (200 µM) for 24 h, rep6

description: Library name: E1_7_24_Hb200_F3


title: BSC, stimulated with hemoglobin (400 µM) for 48 h, rep2

description: Library name: E1_53_48_Hb400_F2


title: BSC, stimulated with hemoglobin (200 µM) for 48 h

description: Library name: E1_47_48_Hb200_F5


title: BSC, stimulated with hemoglobin (400 µM) for 48 h, rep3

description: Library name: E1_58_48_Hb400_F1


title: BSC, stimulated with hemoglobin (400 µM) for 24 h, rep4

description: Library name: E1_43_24_Hb400_M5


title: BSC, control (0 µM, 48 h), rep2

description: Library name: E1_61_48_ctrl_F1


title: BSC, stimulated with hemoglobin (400 µM) for 48 h

description: Library name: E1_48_48_Hb400_F5


title: BSC, stimulated with hemoglobin (200 µM) for 48 h, rep6

description: Library name: E1_87_48_Hb200_M4


title: BSC, control (0 µM, 48 h)

description: Library name: E1_46_48_ctrl_F5


title: BSC, stimulated with hemoglobin (400 µM) for 24 h, rep3

description: Library name: E1_38_24_Hb400_M4


title: BSC, control (0 µM, 24 h), rep5

description: Library name: E1_36_24_ctrl_M4


title: BSC, stimulated with hemoglobin (200 µM) for 24 h, rep3

description: Library name: E1_22_24_Hb200_F4


title: BSC, control (0 µM, 24 h), rep6

description: Library name: E1_41_24_ctrl_M5


title: BSC, stimulated with hemoglobin (200 µM) for 24 h, rep5

description: Library name: E1_42_24_Hb200_M5


title: BSC, stimulated with hemoglobin (200 µM) for 24 h, rep4

description: Library name: E1_32_24_Hb200_M3


title: BSC, control (0 µM, 24 h), rep3

description: Library name: E1_16_24_ctrl_F4


title: BSC, control (0 µM, 24 h), rep4

description: Library name: E1_31_24_ctrl_M3


title: BSC, control (0 µM, 48 h), rep4

description: Library name: E1_76_48_ctrl_M1


title: BSC, stimulated with hemoglobin (400 µM) for 24 h, rep2

description: Library name: E1_3_24_Hb400_F2


title: BSC, stimulated with hemoglobin (400 µM) for 24 h

description: Library name: E1_13_24_Hb400_F3


title: BSC, stimulated with hemoglobin (400 µM) for 48 h, rep4

description: Library name: E1_73_48_Hb400_M1


title: BSC, control (0 µM, 24 h), rep2

description: Library name: E1_11_24_ctrl_F3


title: e3LL_RMC-6291+RMC-6236_8day_rep5

city: Redwood City

first: Ximo

last: Pechuan-Jorge

state: CA

address: 700 Saginaw Drive

country: USA

institute: Revolution Medicines

description: Library name: 5_WH6238_8d

data_processing:
Sequence reads aligned using salmon v1.10
CDX models were demoused by mapping to bother human and mouse, and subsequently removing mouse reads.
Human mapped to reference hg38
Mouse mapped to reference mm39
Read count and normalization performed using edgeR limma-voom.
Assembly: hg38, mm39
Supplementary files format and content: Table of raw counts formatted as a .csv file

source_name: Syngeneic tumor

extract_protocol:
NCI-H2122 CDX samples had RNA was extracted using RNeasy Mini Kit (Qiagen, 74106). Mouse 3LL NRAS KO #86 samples had RNA extracted using Qiagen miRNeasy mini.
NCI-H2122 CDX samples were prepared using directional mRNA library preparation (poly A enrichment). Mouse 3LL NRAS KO #86 samples were prepared using Illumina TruSeq Stranded mRNA, 50M, 100bp PE.

value: Syngeneic tumor

tag: cell line

value: 3LL NRAS KO #86

value: NSCLC


title: BSC, stimulated with hemoglobin (200 µM) for 24 h

description: Library name: E1_12_24_Hb200_F3


title: e3LL_RMC-6291+RMC-6236_8day_rep1

description: Library name: 5_GQ2779_8d


title: e3LL_RMC-6236_8day_rep5

description: Library name: 3_WH6236_8d


title: e3LL_RMC-6236_8day_rep4

description: Library name: 3_WH6228_8d


title: e3LL_RMC-6291+RMC-6236_8day_rep2

description: Library name: 5_GQ2783_8d


title: e3LL_RMC-6291+RMC-6236_8day_rep3

description: Library name: 5_RC7112_8d


title: e3LL_RMC-6236_8day_rep1

description: Library name: 3_BM9643_8d


title: e3LL_RMC-6236_8day_rep2

description: Library name: 3_RC7114_8d


title: e3LL_RMC-6236_8day_rep3

description: Library name: 3_WH6222_8d


title: e3LL_RMC-6291_8day_rep5

description: Library name: 2_LX3165_8d


title: BSC, stimulated with hemoglobin (200 µM) for 24 h, rep2

description: Library name: E1_2_24_Hb200_F2


title: e3LL_RMC-6291_8day_rep4

description: Library name: 2_GQ2780_8d


title: e3LL_RMC-6291_8day_rep3

description: Library name: 2_GQ2765_8d


title: BSC, control (0 µM, 24 h)

description: Library name: E1_1_24_ctrl_F2


title: e3LL_vehicle_8day_rep4

description: Library name: 1_LV6618_8d

value: Vehicle


title: e3LL_vehicle_8day_rep5

description: Library name: 1_WH6232_8d


title: e3LL_RMC-6291_8day_rep1

description: Library name: 2_BJ8450_8d


title: e3LL_vehicle_8day_rep3

description: Library name: 1_LV6608_8d


title: e3LL_RMC-6291_8day_rep2

description: Library name: 2_GQ2760_8d


title: e3LL_RMC-6291+RMC-6236_8day_rep4

description: Library name: 5_WH6221_8d


title: e3LL_vehicle_8day_rep1

description: Library name: 1_GN7869_8d


title: Macrophages from Piezo1^flox/flox C57BL/6J mice following lipopolysaccharide (LPS) treatment-3

city: kunming

first: yunnhan

last: zhang

address: 528 Shahe Road

country: China

institute: Fuwai Yunnan Cardiovascular  Hospital

description:
Library name: Sample3
C3

data_processing:
Cytoscape v3.8.2
Alignment with Reference Genome
Quantification of Gene Abundance
Differentially expressed genes (DEGs) Analysis
Assembly: GRCm39
Supplementary files format and content: raw counts for each sample

source_name: Bone Marrow Derived Macrophages

growth_protocol: Bone Marrow Derived Macrophages are cultured in macrophage medium

extract_protocol:
Total RNA was extracted using Trizol reagent (thermofisher, 15596018) following the manufacturer's procedure. The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA, 5067-1511) , high-quality RNA samples with RIN number > 7.0 were used to construct sequencing library.
After total RNA   was extracted, mRNA was purified from total RNA (5ug) using Dynabeads Oligo (dT) (Thermo Fisher, CA, USA) with two   rounds of purification.    Then the   cleaved RNA fragments were reverse-transcribed to create the cDNA by SuperScript™ II Reverse Transcriptase (Invitrogen,   cat.    1896649, USA), which were next used to synthesise U-labeled second-stranded DNAs with E. coli DNA polymerase I  (NEB, cat.   m0209, USA), RNase H (NEB, cat.   m0297, USA) and dUTP Solution (Thermo Fisher, cat.   R0133, USA).   The average insert size for the final   cDNA librarys were 300±50 bp.    At last, we performed the 2×150bp paired-end sequencing (PE150) on an Illumina  Novaseq™ 6000 following the vendor's recommended protocol.

treatment_protocol: Opting for a dose of 12 mg/kg LPS for our mice model, Bone Marrow Derived Macrophages were isolated from the femurs and tibiae of the mice.

value: Bone Marrow Derived Macrophages

value: Primary cells are from an organism

tag: genotype

value: WT

value: lipopolysaccharide (LPS) treatment.


title: Macrophages from Piezo1^flox/flox C57BL/6J mice following lipopolysaccharide (LPS) treatment-1

description:
Library name: Sample1
C1


title: AML

city: Columbus

first: Bethany

middle: L

last: Mundy-Bosse

state: OH

address: 460 W 12th Ave

department: Department of Internal Medicine

institute: The Ohio State University

description:
Library name: Sample 2
10X Genomics

library_source: transcriptomic single cell

data_processing:
Raw fastq sequences were aligned using the 10X Genomic Cloud Services CellRanger Suite (10X Genomics).
Assembly: Mouse mm10
Supplementary files format and content: Barcodes, features, matrix files
Supplementary files format and content: Integrated Seurat object

source_name: Bone marrow

growth_protocol: PTD/ITD leukemic spleens were harvested at moribund and serial passaged once through recipient congenic mice to enrich for leukemic cells prior to this experiment

extract_protocol:
Non-leukemic recipient cells were enriched through CD45.1 positive selection followed by lineage depletion. Lin- cells were further purified through cell sorting prior to library preparation
Cells were loaded onto a 10X Chromium Controller and prepared for scRNA-seq according to the manufacturer protocol, including library generation (10X Genomics, v3.1). Next Generation Sequencing was performed by the Genomics Core at Nationwide Children’s Hospital using 2x150 bp reads on a NovaSeq SP instrument.

treatment_protocol: Congenic recipient mice were sublethally irradiated with 400 cGy before being adoptively transferred with either 250,000 PTD/ITD splenic cells or PBS vehicle control. Mice were monitored for engraftment through serial cheek bleeds followed by flow cytometry and were harvested approximately 3 weeks following injection.

value: Bone marrow

value: Innate lymphocytes

value: Mll-PTD; Flt3-ITD (PTD/ITD)

tag: Sex

value: pooled male and female

tag: age

value: 12 weeks


title: Macrophages from Piezo1^flox/flox C57BL/6J mice following lipopolysaccharide (LPS) treatment-2

description:
Library name: Sample2
C2


title: Macrophages from Piezo1^flox/flox Lyz2-Cre C57BL/6J mice following lipopolysaccharide (LPS) treatment-2

description:
Library name: Sample5
M2

value: Piezo1 Conditional knockout


title: Healthy

description:
Library name: Sample 1
10X Genomics


title: Macrophages from Piezo1^flox/flox Lyz2-Cre C57BL/6J mice following lipopolysaccharide (LPS) treatment-3

description:
Library name: Sample6
M3


title: Macrophages from Piezo1^flox/flox Lyz2-Cre C57BL/6J mice following lipopolysaccharide (LPS) treatment-1

description:
Library name: Sample4
M1


title: B16_GM_CDOT_1

city: New York

first: Genomics-core-facility

last: WCM

state: NY

address: 1300 York Ave.

institute: Weill Cornell Medicine

description: Library name: Sample 4

data_processing:
The raw sequencing reads in BCL format underwent processing through bcl2fastq v2.20 (Illumina) for FASTQ conversion and demultiplexing.
Following adaptor trimming with Cutadapt v1.18,90 RNA reads were aligned and mapped to the GRCm39 mouse reference genome using STAR v2.5.2.91 Read counts per gene were extracted using HTSeq-count v0.11.2.92
Assembly: GRCm39
Supplementary files format and content: tab-delimited txt file includes gene counts for each sample

extract_protocol:
RNA was extracted from FFPE sections using the Quick RNA-FFPE kit (Zymo Research catalog #R1008) when tissue samples were limited. Reverse transcription was carried out using the High-Capacity RNA-to-cDNA™ Kit (ThermoFisher catalog #4387406) using between 100ng – 1ug of RNA per the manufacturer’s instructions.
Following RNA isolation, FFPE total RNA integrity was checked using a 2100 Bioanalyzer with a DV200 reading as 47% - 61% for all six samples (Agilent Technologies, Santa Clara, CA). RNA concentrations were measured using the NanoDrop system (Thermo Fisher Scientific, Inc., Waltham, MA).  We used 200ng of FFPE total RNA sample as the input for fragmentation by treating with metal ions present in the 2X Priming Buffer at elevated temperature (Agilent SureSelect XT HS2 RNA Library Preparation and Target Enrichment Protocol #G9989-90000, Agilent Technologies, Santa Clara, CA). Fragmented RNA was used to perform the first and the second-strand cDNA synthesis. After AmpPure XP Beads purification, cDNA samples proceeded to end repair, A-tailing and adapter ligation following the manufacturer instructions. The adaptor-ligated cDNA library was then PCR amplified and hybridized by SureSelect XT Mouse All Exon probes (Agilent Technologies, Catalog #5190-4641). Captured and target-enriched cDNA libraries were amplified and purified.

value: C' dot treated


title: B16_GM_CDOT_2

description: Library name: Sample 5


title: B16_F10_CT_2

description: Library name: Sample 8

value: Untreated


title: B16_F10_CDOT_2

description: Library name: Sample 10


title: B16_GM_CT_3

description: Library name: Sample 3


title: B16_F10_CDOT_3

description: Library name: Sample 11


title: B16_F10_CT_1

description: Library name: Sample 7


title: B16_GM_CDOT_3

description: Library name: Sample 6


title: B16_GM_CT_1

description: Library name: Sample 1


title: B16_GM_CT_2

description: Library name: Sample 2


title: e3LL_vehicle_8day_rep2

description: Library name: 1_GQ2784_8d


title: BSC, stimulated with heme-albumin (50 µM) for 24 h, rep10

description: Library name: W53_heme-alb_50

value: brain

value: brain slice cultur


title: BSC, stimulated with heme-albumin (400 µM) for 24 h, rep10

description: Library name: W55_heme-alb_400


title: BSC, control (0 µM, 24 h), rep21

description: Library name: W51_ctrl_0


title: BSC, stimulated with heme-haptoglobin (200 µM) for 24 h, rep9

description: Library name: W49_heme-hpx_200


title: BSC, stimulated with heme-albumin (200 µM) for 24 h, rep10

description: Library name: W54_heme-alb_200


title: BSC, stimulated with heme-albumin (400 µM) for 24 h, rep9

description: Library name: W48_heme-alb_400


title: BSC, stimulated with heme-albumin (200 µM) for 24 h, rep9

description: Library name: W47_heme-alb_200


title: BSC, stimulated with heme-haptoglobin (200 µM) for 24 h, rep8

description: Library name: W46_heme-hpx_200


title: B16_F10_CDOT_1

description: Library name: Sample 9


title: BSC, stimulated with heme-albumin (12.5 µM) for 24 h, rep9

description: Library name: W42_heme-alb_12-5


title: BSC, stimulated with heme-haptoglobin (200 µM) for 24 h, rep7

description: Library name: W36_heme-hpx_200


title: BSC, control (0 µM, 24 h), rep20

description: Library name: W41_ctrl_0


title: BSC, stimulated with heme-albumin (50 µM) for 24 h, rep9

description: Library name: W43_heme-alb_50


title: BSC, stimulated with heme-albumin (12.5 µM) for 24 h, rep8

description: Library name: W37_heme-alb_12-5


title: BSC, stimulated with heme-albumin (400 µM) for 24 h, rep8

description: Library name: W45_heme-alb_400


title: BSC, stimulated with heme-albumin (50 µM) for 24 h, rep8

description: Library name: W38_heme-alb_50


title: BSC, stimulated with heme-albumin (200 µM) for 24 h, rep8

description: Library name: W44_heme-alb_200


title: BSC, stimulated with heme-haptoglobin (200 µM) for 24 h, rep10

description: Library name: W56_heme-hpx_200


title: BSC, stimulated with heme-albumin (12.5 µM) for 24 h, rep10

description: Library name: W52_heme-alb_12-5


title: BSC, stimulated with heme-albumin (400 µM) for 24 h, rep7

description: Library name: W35_heme-alb_400


title: BSC, stimulated with heme-albumin (12.5 µM) for 24 h, rep6

description: Library name: W12_heme-alb_12-5


title: BSC, stimulated with heme-haptoglobin (200 µM) for 24 h, rep6

description: Library name: W16_heme-hpx_200


title: BSC, stimulated with heme-albumin (400 µM) for 24 h, rep6

description: Library name: W15_heme-alb_400


title: BSC, control (0 µM, 24 h), rep19

description: Library name: W31_ctrl_0


title: BSC, control (0 µM, 24 h), rep18

description: Library name: W17_ctrl_0


title: BSC, stimulated with heme-albumin (50 µM) for 24 h, rep6

description: Library name: W13_heme-alb_50


title: BSC, stimulated with heme-albumin (12.5 µM) for 24 h, rep7

description: Library name: W32_heme-alb_12-5


title: BSC, stimulated with heme-albumin (400 µM) for 24 h, rep5

description: Library name: M56_heme-alb_400


title: BSC, stimulated with heme-albumin (50 µM) for 24 h, rep7

description: Library name: W33_heme-alb_50


title: BSC, control (0 µM, 24 h), rep16

description: Library name: M52_ctrl_0


title: BSC, stimulated with heme-albumin (200 µM) for 24 h, rep5

description: Library name: M55_heme-alb_200


title: BSC, control (0 µM, 24 h), rep17

description: Library name: W11_ctrl_0


title: BSC, stimulated with heme-albumin (12.5 µM) for 24 h, rep5

description: Library name: M53_heme-alb_12-5


title: BSC, stimulated with heme-albumin (200 µM) for 24 h, rep6

description: Library name: W14_heme-alb_200


title: BSC, stimulated with heme-haptoglobin (200 µM) for 24 h, rep4

description: Library name: M46_heme-hpx_200


title: BSC, stimulated with heme-haptoglobin (200 µM) for 24 h, rep5

description: Library name: M57_heme-hpx_200


title: BSC, stimulated with heme-albumin (200 µM) for 24 h, rep4

description: Library name: M44_heme-alb_200


title: BSC, stimulated with heme-albumin (50 µM) for 24 h, rep4

description: Library name: M43_heme-alb_50


title: BSC, stimulated with heme-albumin (12.5 µM) for 24 h, rep4

description: Library name: M42_heme-alb_12-5


title: BSC, stimulated with heme-haptoglobin (200 µM) for 24 h, rep3

description: Library name: M36_heme-hpx_200


title: BSC, control (0 µM, 24 h), rep15

description: Library name: M41_ctrl_0


title: BSC, stimulated with heme-albumin (400 µM) for 24 h, rep4

description: Library name: M45_heme-alb_400


title: BSC, stimulated with heme-albumin (200 µM) for 24 h, rep7

description: Library name: W34_heme-alb_200


title: BSC, stimulated with heme-albumin (400 µM) for 24 h, rep3

description: Library name: M35_heme-alb_400


title: BSC, stimulated with heme-albumin (50 µM) for 24 h, rep5

description: Library name: M54_heme-alb_50


title: BSC, control (0 µM, 24 h), rep14

description: Library name: M31_ctrl_0


title: BSC, stimulated with heme-albumin (50 µM) for 24 h, rep3

description: Library name: M33_heme-alb_50


title: BSC, stimulated with heme-albumin (200 µM) for 24 h, rep3

description: Library name: M34_heme-alb_200


title: BSC, stimulated with heme-albumin (400 µM) for 24 h, rep2

description: Library name: M26_heme-alb_400


title: BSC, stimulated with heme-albumin (12.5 µM) for 24 h, rep2

description: Library name: M22_heme-alb_12-5


title: BSC, control (0 µM, 24 h), rep13

description: Library name: M21_ctrl_0


title: BSC, stimulated with heme-haptoglobin (200 µM) for 24 h

description: Library name: M16_heme-hpx_200


title: BSC, stimulated with heme-albumin (12.5 µM) for 24 h, rep3

description: Library name: M32_heme-alb_12-5


title: BSC, stimulated with heme-albumin (200 µM) for 24 h, rep2

description: Library name: M25_heme-alb_200


title: BSC, stimulated with heme-haptoglobin (200 µM) for 24 h, rep2

description: Library name: M28_heme-hpx_200


title: BSC, stimulated with heme-albumin (12.5 µM) for 24 h

description: Library name: M12_heme-alb_12-5


title: BSC, stimulated with heme-albumin (400 µM) for 24 h

description: Library name: M15_heme-alb_400


title: BSC, stimulated with heme-albumin (200 µM) for 24 h

description: Library name: M14_heme-alb_200


title: BSC, stimulated with heme-albumin (50 µM) for 24 h

description: Library name: M13_heme-alb_50


title: BSC, control (0 µM, 24 h), rep12

description: Library name: M11_ctrl_0


title: UPEC1, cauda, day28

city: Giessen

first: Marek

last: Bartkuhn

state: Hessen

address: Aulweg 132

department: Biomedical Informatics and Systems Medicine

country: Germany

institute: Justus-Liebig-University Giessen

description: Library name: UPEC_1

data_processing:
Demutliplexing and FASTQ extraction was performed using bcl2fastq v2.20.0.422.
Demutliplexing and FASTQ extraction was performed using bcl2fastq v2.20.0.422.
Primary processing of FASTQ data including quality control, filtering, trimming,alignment of reads and counting of gene-sprecific genes was done using nf-core RNA-seq pipeline v3.7
Assembly: mm10
Assembly: mm10
Supplementary files format and content: RNA-seq read counts per gene (csv)

molecule: polyA RNA

source_name: cauda epdidymidis

growth_protocol: Uropathogenic E. coli (UPEC) strain CFT073 (Welch et al., 2002) was provided by the Institute of Medical Microbiology (Justus-Liebig-University Giessen, Germany).

extract_protocol:
RNA was extracted from mouse cauda epididymidis using the RNeasy Mini Kit according to the manufacturer’s instructions
NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina®

treatment_protocol: To elicit an ascending infection, vasa deferentia were bilaterally ligated followed by an intravasal injection of 5 µl sterile 0.9% NaCl containing 1-2 x 105 colony forming units proximal to the ligation using a Hamilton syringe (Pleuger et al., 2022). Control ‘sham’ mice were subjected to the same surgical procedure, but with an intravasal injection of 5 µl sterile 0.9% NaCl without bacteria. Mice were sacrificed  by isoflurane inhalation followed by cervical dislocation after 28 days from the infection. The cauda epididymidis was collected and

value: cauda epdidymidis

tag: time

value: 28 days

tag: infection

value: UPEC


title: UPEC3, cauda, day28

description: Library name: UPEC_3


title: UPEC2, cauda, day28

description: Library name: UPEC_2


title: sham3, cauda, day28

description: Library name: Sham_3

value: sham


title: sham2, cauda, day28

description: Library name: Sham_2


title: sham1, cauda, day28

description: Library name: Sham_1


title: BSC, stimulated with heme-albumin (50 µM) for 24 h, rep2

description: Library name: M23_heme-alb_50


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-P11

city: La Jolla

first: Joseph

middle: R

last: Ecker

address: 10010 North Torrey Pines Road

department: Genomic Analysis Laboratory

institute: HHMI-Salk-Institute

library_source: genomic

data_processing:
Library strategy: snmC-seq3
Reads trimed with Cutadapt for adapters and low quality bases (Phred score >= 20)
The resulting files are mapped to in-silico bisulfite converted reference genome using Bismark.
The raw BAM file will be filtered using samtools to filter reads by MAPQ > 10 and use Picard to remove PCR duplicates
Tab-delimited files (ALLC files) describing the quantity of unmethylated and methylated cytosine base calls will be generated with in-house developing package using the filtered BAM files
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited files (ALLC files) describing the quantity of unmethylated and methylated cytosine base calls. Columns are: 1. Chromosome, 2. Position, 3. Strand, 4. Cytosin Context, 5. methylated base call, 6. total mapped base call, 7. not used, place holder column

molecule: genomic DNA

extract_protocol:
Regions of human brains were dissected.
Library prepared following protocol discribed in Luo, Chongyuan, Angeline Rivkin, Jingtian Zhou, Justin P. Sandoval, Laurie Kurihara, Jacinta Lucero, Rosa Castanon, et al. 2018. “Robust Single-Cell DNA Methylome Profiling with snmC-seq2.” Nature Communications 9 (1): 3824.

value: 9 months

tag: brain region

value: FC

tag: gender

value: M


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-O24


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-O23


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-M23


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-O11


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-N11


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-P12


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-K24


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-O12


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-P23


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-N24


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-P24


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-N23


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-L12


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-M24


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-K23


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-J24


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-K11


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-M11


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-K12


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-J23


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-L24


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-N12


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-J12


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-J11


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-L23


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-L11


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-I23


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-H24


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-G23


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-H11


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-G24


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-F24


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-I11


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-H12


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-H23


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-G12


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-F12


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-F23


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-F11


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-E23


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-E12


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-E11


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-I24


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-D24


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-C24


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-M12


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-D11


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-E24


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-C23


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-G11


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-C12


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-I12


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-B24


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-C11


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-B23


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-P22


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-D12


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-D23


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-B12


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-A24


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-P9


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-A23


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-P21


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-B11


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-A12


title: AMB_200702_9mo_2A_3A_2_P4-6-J5-A11


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-P10


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-N21


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-O9


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-M22


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-O22


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-N22


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-N9


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-L22


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-M21


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-N10


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-L9


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-L21


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-L10


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-K9


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-O21


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-K22


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-J22


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-J21


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-K21


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-I10


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-J10


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-H9


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-K10


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-H21


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-O10


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-M9


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-J9


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-I21


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-I9


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-G22


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-M10


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-G10


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-H22


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-G9


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-G21


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-F9


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-F22


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-F10


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-F21


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-H10


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-E9


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-E22


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-E21


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-E10


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-D22


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-D9


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-I22


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-C9


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-D10


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-D21


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-C10


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-B9


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-C21


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-B22


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-B21


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-B10


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-P8


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-A21


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-A10


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-C22


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-A22


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-P20


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-O8


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-P19


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-O20


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-P7


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-O19


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-O7


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-N20


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-N7


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-N19


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-M8


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-M7


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-M20


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-M19


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-L8


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-L20


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-J19


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-K7


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-I19


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-K20


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-K19


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-L19


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-G8


title: AMB_200702_9mo_2A_3A_2_P4-5-D7-A9


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-K8


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-J20


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-I7


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-G20


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-J7


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-J8


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-H8


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-H7


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-H19


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-I8


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-G7


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-F20


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-F7


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-G19


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-F19


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-N8


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-E8


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-F8


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-E7


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-L7


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-I20


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-H20


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-E20


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-E19


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-D7


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-D20


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-D19


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-C8


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-B8


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-C20


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-B7


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-B20


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-A19


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-C7


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-A7


title: AMB_200702_9mo_2A_3A_2_P4-3-N12-P6


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-B19


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-A8


title: AMB_200702_9mo_2A_3A_2_P4-4-L19-A20


title: AMB_200702_9mo_2A_3A_2_P4-3-N12-O18


title: AMB_200702_9mo_2A_3A_2_P4-3-N12-P5


title: AMB_200702_9mo_2A_3A_2_P4-3-N12-O6


title: AMB_200702_9mo_2A_3A_2_P4-3-N12-O5


title: AMB_200702_9mo_2A_3A_2_P4-3-N12-N6


title: AMB_200702_9mo_2A_3A_2_P4-3-N12-P17


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title: AMB_200702_9mo_2A_3A_2_P1-1-K22-F2


title: AMB_200702_9mo_2A_3A_2_P1-1-K22-F14


title: AMB_200702_9mo_2A_3A_2_P1-1-K22-F13


title: AMB_200702_9mo_2A_3A_2_P1-1-K22-E2


title: AMB_200702_9mo_2A_3A_2_P1-1-K22-F1


title: AMB_200702_9mo_2A_3A_2_P1-1-K22-E14


title: AMB_200702_9mo_2A_3A_2_P1-1-K22-J13


title: AMB_200702_9mo_2A_3A_2_P1-1-K22-P13


title: AMB_200702_9mo_2A_3A_2_P1-1-K22-J1


title: AMB_200702_9mo_2A_3A_2_P1-1-K22-D2


title: AMB_200702_9mo_2A_3A_2_P1-1-K22-D14


title: AMB_200702_9mo_2A_3A_2_P1-1-K22-E13


title: AMB_200702_9mo_2A_3A_2_P1-1-K22-C2


title: AMB_200702_9mo_2A_3A_2_P1-1-K22-D13


title: AMB_200702_9mo_2A_3A_2_P1-1-K22-E1


title: AMB_200702_9mo_2A_3A_2_P1-1-K22-C13


title: AMB_200702_9mo_2A_3A_2_P1-1-K22-C1


title: AMB_200702_9mo_2A_3A_2_P1-1-K22-H14


title: AMB_200702_9mo_2A_3A_2_P1-1-K22-B14


title: AMB_200702_9mo_2A_3A_2_P1-1-K22-B2


title: AMB_200702_9mo_2A_3A_2_P1-1-K22-C14


title: AMB_200702_9mo_2A_3A_2_P1-1-K22-B1


title: AMB_200702_9mo_2A_3A_2_P1-1-K22-H1


title: AMB_200702_9mo_2A_3A_2_P1-1-K22-B13


title: AMB_200702_9mo_2A_3A_2_P1-1-K22-A2


title: AMB_200702_9mo_2A_3A_2_P1-1-K22-N2


title: AMB_200702_9mo_2A_3A_2_P1-1-K22-A13


title: AMB_200702_9mo_2A_3A_2_P1-1-K22-A14


title: AMB_200630_9mo_2A_3A_1_P4-6-C19-P23


title: AMB_200702_9mo_2A_3A_2_P1-1-K22-D1


title: AMB_200702_9mo_2A_3A_2_P1-1-K22-A1


title: AMB_200630_9mo_2A_3A_1_P4-6-C19-P12


title: AMB_200630_9mo_2A_3A_1_P4-6-C19-P24


title: AMB_200630_9mo_2A_3A_1_P4-6-C19-O12


title: AMB_200630_9mo_2A_3A_1_P4-6-C19-P11


title: AMB_200630_9mo_2A_3A_1_P4-6-C19-O23


title: AMB_200630_9mo_2A_3A_1_P4-6-C19-N23


title: AMB_200630_9mo_2A_3A_1_P4-6-C19-N12


title: AMB_200630_9mo_2A_3A_1_P4-6-C19-N24


title: AMB_200630_9mo_2A_3A_1_P4-6-C19-M24


title: AMB_200630_9mo_2A_3A_1_P4-6-C19-M23


title: AMB_200630_9mo_2A_3A_1_P4-6-C19-N11


title: AMB_200630_9mo_2A_3A_1_P4-6-C19-M12


title: AMB_200630_9mo_2A_3A_1_P4-6-C19-M11


title: AMB_200630_9mo_2A_3A_1_P4-6-C19-L23


title: AMB_200630_9mo_2A_3A_1_P4-6-C19-L12


title: AMB_200630_9mo_2A_3A_1_P4-6-C19-L11


title: AMB_200630_9mo_2A_3A_1_P4-6-C19-K24


title: AMB_200630_9mo_2A_3A_1_P4-6-C19-L24


title: AMB_200630_9mo_2A_3A_1_P4-6-C19-K23


title: AMB_200630_9mo_2A_3A_1_P4-6-C19-J24


title: AMB_200630_9mo_2A_3A_1_P4-6-C19-K11


title: AMB_200630_9mo_2A_3A_1_P4-6-C19-J23


title: AMB_200630_9mo_2A_3A_1_P4-6-C19-K12


title: AMB_200630_9mo_2A_3A_1_P4-6-C19-J11


title: AMB_200630_9mo_2A_3A_1_P4-6-C19-O11


title: AMB_200630_9mo_2A_3A_1_P4-6-C19-O24


title: AMB_200630_9mo_2A_3A_1_P4-6-C19-I23


title: AMB_200630_9mo_2A_3A_1_P4-6-C19-I12