title: CAVD-3

city: Guangzhou

first: Qingchun

last: Zeng

state: Guangdong Province

address: 1838 Northern Guangzhou Ave

department: State Key Laboratory of Organ Failure Research, Department of Cardiology

institute: Nanfang Hospital, Southern Medical University

description: Library name: Sample3

data_processing:
The raw reads were filtered by Seqtk before mapping to genome using Hisat2 (version:2.0.4)
The fragments of genes were counted using stringtie(v1.3.0) followed by TMM (trimmed mean of M values) normalization
Significant differential expressed genes (DEGs) were identified as those with a False Discovery Rate (FDR) value above the threshold (Q< 0.05) and fold-change >2 using edgeR software
Assembly: GRCh38

source_name: Aortic Valve

extract_protocol:
RNA was harvested and extracted using MJzol animal RNA Extraction Kit(MagBeads) (Cat#T02096).
RNA library for RNA-seq was prepared using SMARTer Stranded Total RNA-Seq Kit v2 following manufacturer's protocol.

value: Aortic Valve

tag: condition

value: calcific aortic valve disease


title: CAVD-4

status: Public on Dec 15 2025

type: SRA

country: China

description: Library name: Sample4

library_source: transcriptomic

molecule: total RNA

organism: Homo sapiens

tag: tissue


title: CAVD-5

description: Library name: Sample5


title: non-CAVD-4

description: Library name: Sample10

value: non-calcific aortic valves


title: non-CAVD-5

description: Library name: Sample11


title: Niche 3

city: Shanghai

first: Chuqing

last: He

address: No. 910, Hengshan Rd

department: Department of Obstetrics and Gynecology

institute: School of Medicine, Shanghai Jiaotong University

description:
Library name: sample 1
10x Genomics

library_source: transcriptomic single cell

data_processing:
Single-cell analysis was conducted using Seurat (version 4.3.0). Potential doublets in the single-cell data were identified and removed using DoubleFinder (version 2.0) (available from https://github.com/chris-mcginnis-ucsf/DoubletFinder).
Genes included in the analysis were required to be expressed in a minimum of three cells, with the total number of expressed cells that expressed >200 genes and <5000 genes. Cells with a high percentage of mitochondrial genes to total genes (> 20%) and hemoglobin genes (> 2%) were filtered out.
Qualified cells from Adjacent tissues (n = 3),Control tissues (n = 4), and Niche tissues (n = 3) were used for scRNA-seq analysis.Using Seurat, the top 2000 most variable genes were identified as highly variable genes (HVG) based on their average expression and dispersion. The dimensionality of the data was reduced using principal component analysis (PCA) on HVG. The Louvain modularity optimization algorithm was used to cluster the cells in the graph, and the clustering results were visualized using Uniform Manifold Approximation and Projection (UMAP).
Differential expression and enrichment analysis were performed to explore the cellular heterogeneity of fibroblasts and endothelial cells.
Trajectory analysis was performed to explore endothelial cells transition.
Assembly: hg38
Supplementary files format and content: *cellranger_barcodes.tsv
Supplementary files format and content: *cellranger_matrix.mtx
Supplementary files format and content: *cellranger_features.tsv

molecule: polyA RNA

source_name: uterine niche

extract_protocol:
Adjacent myometrium tissue of uterine niche (Adjacent3,Adjacent4&Adjacent5), well-healed cesarean scar tissue (Control2,Control3,Control4&Control5) and uterine niche tissue (Niche3,,Niche4 & Niche5) were harvested. These tissues were cut into small pieces with scissors and then incubated in DMEM medium containing collagenase I (Gibco, 17100017) and collagenase II (Gibco, 17101015) at 37°C with gentle shaking for 40-60 minutes. The digested suspension was filtered through a 100-µm cell strainer (CSS-013-100, BIOFIL). Then, the enzymatic reaction was stopped by adding 10% fetal bovine serum (FBS). The cells were pelleted by centrifugation at 350 × g for 5 minutes at 4°C. Red blood cell lysis buffer (Beyotime Biotech, C3702) was used to remove red blood cells. After removing the supernatant, the cells were resuspended in 100 μL of DMEM medium. Cell analysis was conducted using a Trypan blue cell counter, and the cell viability was consistently above 80%. The cell concentration for loading onto the 10x Genomics Chromium™ machine ranged from 700 cells/μL to 1200 cells/μL. Cells were counted and loaded onto the Chromium Controller (10X Genomics) to recover a target of 8000 single cells. The process involved the generation of gel beads in emulsion (GEMs), barcoding, GEM-RT clean-up, complementary DNA amplification, and library construction. Library quantification was performed using Qubit before pooling. The final library pool was sequenced on an Illumina Nova6000 platform using 150-base-pair paired-end reads. Aligning to the GRCh38 human genome, Cell Ranger software (version 6.0.3) was used for quality control and read counting of Ensembl genes
The cell concentration for loading onto the 10x Genomics Chromium™ machine ranged from 700 cells/μL to 1200 cells/μL. Cells were counted and loaded onto the Chromium Controller (10X Genomics) to recover a target of 8000 single cells. The process involved the generation of gel beads in emulsion (GEMs), barcoding, GEM-RT clean-up, complementary DNA amplification, and library construction. Library quantification was performed using Qubit before pooling. The final library pool was sequenced on an Illumina Nova6000 platform using 150-base-pair paired-end reads.Aligning to the GRCh38 human genome, Cell Ranger software (version 6.0.3) was used for quality control and read counting of Ensemble genes.

value: uterine niche


title: pds3_5fu_rna

city: Göteborg

first: Emma

last: Jonasson

address: Medicinaregatan 1F, floor 5

department: Sahlgrenska Center for Cancer Research

country: Sweden

institute: University of Gothenburg

description:
Library name: pds3_5fu_rna
Targeted single-cell triomics analysis using the MissionBio tapestri platform

data_processing:
Data was processed using the Tapestri DNA + Protein v2.0.2 pipeline (MissionBio), modified to include the RNA data.
For DNA, data was processed using the Tapestri DNA + Protein v2.0.2 pipeline (MissionBio). Briefly, adapter trimming was performed using Cutadapt, reads greater than 30 bases were retained, and the barcode structures were extracted. The pipeline identified valid barcodes and barcodes that can be corrected for sequencing errors. Reads were mapped to the hg19 reference genome using the BWA-MEM algorithm. Cell calling was performed using a cut-off for total DNA reads where cells with a percent of DNA amplicons per barcode greater than 0.2X of the mean of all DNA amplicon reads were included. Variant calling in the DNA reads was performed using GATK. Cells were genotyped using the variant calls and cell lines identified from their known genotypes.
For RNA, adapter trimming was performed using Cutadapt, reads greater than 30 bases were retained, and the barcode structures were extracted. The pipeline identified valid barcodes and barcodes that can be corrected for sequencing errors. Reads were mapped to the hg19 reference genome using the STAR algorithm. Target reads that had more than five bases reading into the intron were removed.  RNA reads with cell barcodes corresponding to the cell barcodes identified as cells in the DNA library were used for downstream analysis.
For Proteins, data was processed using the Tapestri DNA + Protein v2.0.2 pipeline (MissionBio). Briefly, antibody barcodes were identified, including all variants with single base substitutions, to account for sequencing errors. Then, cell barcodes were extracted and added to the fastq header and adapters were trimmed. Using the error-corrected map for the antibody barcodes, the antibody barcodes were identified in the reads present in the trimmed fastq file. Subsequently, the capture sequence and PCR handle were also trimmed from the reads. If the remaining read sequence were of the desired length and matched any of the antibody barcodes in the error correction map, it was accepted. Then, the number of reads for each antibody barcode within a cell were counted to generate the final output file listing the cell barcodes and the number of antibody reads for each barcode.
Assembly: hg19
Supplementary files format and content: Triomics_data_processed.csv, processed and filtered data received from the MissionBio pipeline.

source_name: Breast; Mammary gland

growth_protocol: MCF7 cells (ATCC) were cultured in DMEM supplemented with 10 % fetal bovine serum, 1 % penicillin/streptomycin, 1% L-glutamine and 1 % non-essential amino acids . T-47D cells (ATCC) were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum, 1% penicillin/streptomycin, 1% sodium pyruvate and 1% L-glutamine. Cell culture conditions were 37°C and 5% CO2 in humidified atmosphere, and cells were not maintained for longer than 30 passages.

extract_protocol:
Cells from monolayer cultures were washed with PBS and detached with trypsin for 3 min, at 37°C. PDS slices were soaked 3 times in PBS and cells were detached with Corning Accutase for 5 min at 37 °C with gentle agitation at 180 rpm. Single-cell suspensions were then generated using a 25G needle and a 40 µm cell strainer. Dead cell removal was performed using the MACS Dead Cell Removal kit (Miltenyi), according to the manufacturer’s instructions.
The triomics experiment was performed using the Tapestri Single-Cell Multiomics V1.1 workflow (Mission Bio). Briefly, single-cell suspensions were stained using protein-targeting AOCs and subsequently diluted to ~3500 cells/ml. Stained cells were then encapsulated in droplets by the Tapestri instrument. Subsequently, cell lysis, reverse transcription and protease treatment was run. Custom panels for DNA and RNA was used and genomic DNA, cDNA and antibody tag sequences were barcoded in emulsion. Barcoding beads were cleaved off from targeted sequences using UV light. The target sequences were preamplified after which the emulsion was broken and libraries specific for DNA, RNA and protein targets were enriched and purified using Dynabeads MyOne Streptavidin and SPRI beads. Separate libraries for each analyte were then generated by PCR using Illumina primers. Final libraries were purified using SPRI beads before quantification and quality control using capillary gel-electrophoresis on a Fragment Analyzer. Libraries were pooled and sequenced according to the manufacturer’s instructions using 150 paired-end cycles, supplemented with 15% PhiX Control v3.

treatment_protocol: 5-fluorouracil was dissolved in saline solution. For treatment of monolayer cultures, 2 x 105 MCF7 cells and 3 x 105 T-47D cells were seeded in 6-well plates containing 2 ml of cell-specific media. After 24 hours, IC50 concentrations of 5-fluorouracil (100 µM for MCF7 and 200 µM for T-47D cells, respectively) was administrated for 48 hours. For treatment of PDS-cultured cells, 1 mM 5-fluorouracil was administrated for 48 hours.

value: Breast; Mammary gland

tag: cell line

tag: culture

value: PDS

tag: treatment

tag: library type

value: mRNA


title: non-CAVD-2

description: Library name: Sample8


title: pds3_control_protein

description:
Library name: pds3_control_protein
Targeted single-cell triomics analysis using the MissionBio tapestri platform

library_source: other

molecule: protein

value: Control

value: ADT


title: CAVD-1

description: Library name: Sample1


title: non-CAVD-3

description: Library name: Sample9


title: CAVD-2

description: Library name: Sample2


title: pds3_control_rna

description:
Library name: pds3_control_rna
Targeted single-cell triomics analysis using the MissionBio tapestri platform


title: pds3_control_dna

description:
Library name: pds3_control_dna
Targeted single-cell triomics analysis using the MissionBio tapestri platform

library_source: genomic

molecule: genomic DNA

value: DNA


title: pds2_control_protein

description:
Library name: pds2_control_protein
Targeted single-cell triomics analysis using the MissionBio tapestri platform


title: pds2_5fu_protein

description:
Library name: pds2_5fu_protein
Targeted single-cell triomics analysis using the MissionBio tapestri platform


title: pds2_5fu_rna

description:
Library name: pds2_5fu_rna
Targeted single-cell triomics analysis using the MissionBio tapestri platform


title: pds2_control_dna

description:
Library name: pds2_control_dna
Targeted single-cell triomics analysis using the MissionBio tapestri platform


title: Niche 4

description:
Library name: sample 2
10x Genomics


title: pds1_5fu_rna

description:
Library name: pds1_5fu_rna
Targeted single-cell triomics analysis using the MissionBio tapestri platform


title: pds2_control_rna

description:
Library name: pds2_control_rna
Targeted single-cell triomics analysis using the MissionBio tapestri platform


title: pds1_5fu_dna

description:
Library name: pds1_5fu_dna
Targeted single-cell triomics analysis using the MissionBio tapestri platform


title: pds2_5fu_dna

description:
Library name: pds2_5fu_dna
Targeted single-cell triomics analysis using the MissionBio tapestri platform


title: pds1_control_protein

description:
Library name: pds1_control_protein
Targeted single-cell triomics analysis using the MissionBio tapestri platform


title: non-CAVD-1

description: Library name: Sample7


title: pds1_5fu_protein

description:
Library name: pds1_5fu_protein
Targeted single-cell triomics analysis using the MissionBio tapestri platform


title: CAVD-6

description: Library name: Sample6


title: pds3_5fu_protein

description:
Library name: pds3_5fu_protein
Targeted single-cell triomics analysis using the MissionBio tapestri platform


title: pds1_control_dna

description:
Library name: pds1_control_dna
Targeted single-cell triomics analysis using the MissionBio tapestri platform


title: OPR070B,tumor

city: New York

first: Sneha

last: Mitra

state: NY

address: 1275 York Avenue

country: USA

institute: Memorial Sloan Kettering Cancer Center

description:
Library name: Sample_OPR070B
Column name in counts.txt: OPR070B

data_processing:
Sequence reads were mapped using STAR aligner followed by postprocessing using PICARD to generate BAM files.
Assembly: hg38
Supplementary files format and content: counts.txt contains raw counts per sample
Supplementary files format and content: metadata.csv contains survival data

source_name: tumor

extract_protocol:
RNA was extracted from FFPE patient samples using Qiagen AllPrep DNA/RNA FFPE Kit (80234). After RiboGreen quantificaiton and quality control by Agilent BioAnalyzer 0.1-1 µg of total RNA was used for construction of sequencing libraries.
Library prepration was done using TruSeq Stranded Total RNA Kit (Illumina catalog # RS-122-1202) according to instructions provided by the manufacturer with 8 cycles of PCR.  Samples were barcoded and run on a NovaSeq 6000 in a PE100 run, using the NovaSeq 6000 S4 Reagent Kit (200 Cycles) (Illumina).  On average, 96 million paired reads were generated per sample and 40% of the data mapped to the transcriptome

value: tumor

tag: cell type

value: CRC


title: OPR062B,tumor

description:
Library name: Sample_OPR062B
Column name in counts.txt: OPR062B


title: OPR072B,tumor

description:
Library name: Sample_OPR072B
Column name in counts.txt: OPR072B


title: OPR078B,tumor

description:
Library name: Sample_OPR078B
Column name in counts.txt: OPR078B


title: OPR064B,tumor

description:
Library name: Sample_OPR064B
Column name in counts.txt: OPR064B


title: pds3_5fu_dna

description:
Library name: pds3_5fu_dna
Targeted single-cell triomics analysis using the MissionBio tapestri platform


title: OPR061B,tumor

description:
Library name: Sample_OPR061B
Column name in counts.txt: OPR061B


title: OPR052B,tumor

description:
Library name: Sample_OPR052B
Column name in counts.txt: OPR052B


title: OPR048B,tumor

description:
Library name: Sample_OPR048B
Column name in counts.txt: OPR048B


title: OPR057B,tumor

description:
Library name: Sample_OPR057B
Column name in counts.txt: OPR057B


title: OPR050B,tumor

description:
Library name: Sample_OPR050B
Column name in counts.txt: OPR050B


title: OPR044B,tumor

description:
Library name: Sample_OPR044B
Column name in counts.txt: OPR044B


title: pds1_control_rna

description:
Library name: pds1_control_rna
Targeted single-cell triomics analysis using the MissionBio tapestri platform


title: OPR036B,tumor

description:
Library name: Sample_OPR036B
Column name in counts.txt: OPR036B


title: OPR041B,tumor

description:
Library name: Sample_OPR041B
Column name in counts.txt: OPR041B


title: OPR042B,tumor

description:
Library name: Sample_OPR042B
Column name in counts.txt: OPR042B


title: OPR029B,tumor

description:
Library name: Sample_OPR029B
Column name in counts.txt: OPR029B


title: OPR032B,tumor

description:
Library name: Sample_OPR032B
Column name in counts.txt: OPR032B


title: OPR030B,tumor

description:
Library name: Sample_OPR030B
Column name in counts.txt: OPR030B


title: OPR013B,tumor

description:
Library name: Sample_OPR013B
Column name in counts.txt: OPR013B


title: OPR027B,tumor

description:
Library name: Sample_OPR027B
Column name in counts.txt: OPR027B


title: OPR039B,tumor

description:
Library name: Sample_OPR039B
Column name in counts.txt: OPR039B


title: OPR001B,tumor

description:
Library name: Sample_OPR001B
Column name in counts.txt: OPR001B


title: OPR024B,tumor

description:
Library name: Sample_OPR024B
Column name in counts.txt: OPR024B


title: OPR010B,tumor

description:
Library name: Sample_OPR010B
Column name in counts.txt: OPR010B


title: JM349B,tumor

description:
Library name: Sample_JM349B
Column name in counts.txt: JM349B


title: JM346B,tumor

description:
Library name: Sample_JM346B
Column name in counts.txt: JM346B


title: OPR060B,tumor

description:
Library name: Sample_OPR060B
Column name in counts.txt: OPR060B


title: JM343B,tumor

description:
Library name: Sample_JM343B
Column name in counts.txt: JM343B


title: JM334B,tumor

description:
Library name: Sample_JM334B
Column name in counts.txt: JM334B


title: JM337B,tumor

description:
Library name: Sample_JM337B
Column name in counts.txt: JM337B


title: JM328B,tumor

description:
Library name: Sample_JM328B
Column name in counts.txt: JM328B


title: JM338B,tumor

description:
Library name: Sample_JM338B
Column name in counts.txt: JM338B


title: JM325B,tumor

description:
Library name: Sample_JM325B
Column name in counts.txt: JM325B


title: OPR051B,tumor

description:
Library name: Sample_OPR051B
Column name in counts.txt: OPR051B


title: JM320B,tumor

description:
Library name: Sample_JM320B
Column name in counts.txt: JM320B


title: JM329B,tumor

description:
Library name: Sample_JM329B
Column name in counts.txt: JM329B


title: JM314B,tumor

description:
Library name: Sample_JM314B
Column name in counts.txt: JM314B


title: JM313B,tumor

description:
Library name: Sample_JM313B
Column name in counts.txt: JM313B


title: JM306B,tumor

description:
Library name: Sample_JM306B
Column name in counts.txt: JM306B


title: JM291B,tumor

description:
Library name: Sample_JM291B
Column name in counts.txt: JM291B


title: JM304B,tumor

description:
Library name: Sample_JM304B
Column name in counts.txt: JM304B


title: JM299B,tumor

description:
Library name: Sample_JM299B
Column name in counts.txt: JM299B


title: JM290B,tumor

description:
Library name: Sample_JM290B
Column name in counts.txt: JM290B


title: JM288B,tumor

description:
Library name: Sample_JM288B
Column name in counts.txt: JM288B


title: JM289B,tumor

description:
Library name: Sample_JM289B
Column name in counts.txt: JM289B


title: JM310B,tumor

description:
Library name: Sample_JM310B
Column name in counts.txt: JM310B


title: JM281B,tumor

description:
Library name: Sample_JM281B
Column name in counts.txt: JM281B


title: JM305B,tumor

description:
Library name: Sample_JM305B
Column name in counts.txt: JM305B


title: JM280B,tumor

description:
Library name: Sample_JM280B
Column name in counts.txt: JM280B


title: JM284B,tumor

description:
Library name: Sample_JM284B
Column name in counts.txt: JM284B


title: JM321B,tumor

description:
Library name: Sample_JM321B
Column name in counts.txt: JM321B


title: JM274B,tumor

description:
Library name: Sample_JM274B
Column name in counts.txt: JM274B


title: JM279B,tumor

description:
Library name: Sample_JM279B
Column name in counts.txt: JM279B


title: JM275B,tumor

description:
Library name: Sample_JM275B
Column name in counts.txt: JM275B


title: JM324B,tumor

description:
Library name: Sample_JM324B
Column name in counts.txt: JM324B


title: JM322B,tumor

description:
Library name: Sample_JM322B
Column name in counts.txt: JM322B


title: JM271B,tumor

description:
Library name: Sample_JM271B
Column name in counts.txt: JM271B


title: JM269B,tumor

description:
Library name: Sample_JM269B
Column name in counts.txt: JM269B


title: JM268B,tumor

description:
Library name: Sample_JM268B
Column name in counts.txt: JM268B


title: JM316B,tumor

description:
Library name: Sample_JM316B
Column name in counts.txt: JM316B


title: JM265B,tumor

description:
Library name: Sample_JM265B
Column name in counts.txt: JM265B


title: JM264B,tumor

description:
Library name: Sample_JM264B
Column name in counts.txt: JM264B


title: JM262B,tumor

description:
Library name: Sample_JM262B
Column name in counts.txt: JM262B


title: JM256B,tumor

description:
Library name: Sample_JM256B
Column name in counts.txt: JM256B


title: JM258B,tumor

description:
Library name: Sample_JM258B
Column name in counts.txt: JM258B


title: JM253B,tumor

description:
Library name: Sample_JM253B
Column name in counts.txt: JM253B


title: JM248B,tumor

description:
Library name: Sample_JM248B
Column name in counts.txt: JM248B


title: JM252B,tumor

description:
Library name: Sample_JM252B
Column name in counts.txt: JM252B


title: JM247B,tumor

description:
Library name: Sample_JM247B
Column name in counts.txt: JM247B


title: JM249B,tumor

description:
Library name: Sample_JM249B
Column name in counts.txt: JM249B


title: JM245B,tumor

description:
Library name: Sample_JM245B
Column name in counts.txt: JM245B


title: JM250B,tumor

description:
Library name: Sample_JM250B
Column name in counts.txt: JM250B


title: JM246B,tumor

description:
Library name: Sample_JM246B
Column name in counts.txt: JM246B


title: JM243B,tumor

description:
Library name: Sample_JM243B
Column name in counts.txt: JM243B


title: JM241B,tumor

description:
Library name: Sample_JM241B
Column name in counts.txt: JM241B


title: JM244B,tumor

description:
Library name: Sample_JM244B
Column name in counts.txt: JM244B


title: JM272B,tumor

description:
Library name: Sample_JM272B
Column name in counts.txt: JM272B


title: JM242B,tumor

description:
Library name: Sample_JM242B
Column name in counts.txt: JM242B


title: JM270B,tumor

description:
Library name: Sample_JM270B
Column name in counts.txt: JM270B


title: JM237B,tumor

description:
Library name: Sample_JM237B
Column name in counts.txt: JM237B


title: JM238B,tumor

description:
Library name: Sample_JM238B
Column name in counts.txt: JM238B


title: JM240B,tumor

description:
Library name: Sample_JM240B
Column name in counts.txt: JM240B


title: JM236B,tumor

description:
Library name: Sample_JM236B
Column name in counts.txt: JM236B


title: JM232B,tumor

description:
Library name: Sample_JM232B
Column name in counts.txt: JM232B


title: JM234B,tumor

description:
Library name: Sample_JM234B
Column name in counts.txt: JM234B


title: JM235B,tumor

description:
Library name: Sample_JM235B
Column name in counts.txt: JM235B


title: JM231B,tumor

description:
Library name: Sample_JM231B
Column name in counts.txt: JM231B


title: JM226B,tumor

description:
Library name: Sample_JM226B
Column name in counts.txt: JM226B


title: JM224B,tumor

description:
Library name: Sample_JM224B
Column name in counts.txt: JM224B


title: JM259B,tumor

description:
Library name: Sample_JM259B
Column name in counts.txt: JM259B


title: JM222B,tumor

description:
Library name: Sample_JM222B
Column name in counts.txt: JM222B


title: JM223B,tumor

description:
Library name: Sample_JM223B
Column name in counts.txt: JM223B


title: JM221B,tumor

description:
Library name: Sample_JM221B
Column name in counts.txt: JM221B


title: JM220B,tumor

description:
Library name: Sample_JM220B
Column name in counts.txt: JM220B


title: JM212B,tumor

description:
Library name: Sample_JM212B
Column name in counts.txt: JM212B


title: JM214B,tumor

description:
Library name: Sample_JM214B
Column name in counts.txt: JM214B


title: JM215B,tumor

description:
Library name: Sample_JM215B
Column name in counts.txt: JM215B


title: JM213B,tumor

description:
Library name: Sample_JM213B
Column name in counts.txt: JM213B


title: JM210B,tumor

description:
Library name: Sample_JM210B
Column name in counts.txt: JM210B


title: L140P-PUF60 rep4

city: La Jolla

first: Brian

last: Yee

state: CA

address: 9500 Gilman Dr

department: Health

institute: UCSD

description:
L140PvsWT.SE.MATS.JC.txt
L140P_featurecounts_unfiltered.csv
shi1_L140P_plusD_4

data_processing:
Reads were mapped using STAR 2.7.6a
Read count extraction was performed using featureCounts from the Subread package. Results were sorted into counts matrices
TPM was calculated manually from counts matrix
Differential splicing analysis was performed on STAR-aligned reads using rMATS 4.0.2. For each condition, shRNA knockdown samples represent SAMPLE_1, while non-targeting controls (shNT1, shNT2, and shNT3) represent SAMPLE_2.
Assembly: Assembly: GRCh38
Supplementary files format and content: Supplementary files format and content: csv files include TPM values for each sample in comparison to non-targeting shRNA control, along with sample means and standard deviations
Supplementary files format and content: Supplementary files format and content: tab-delimited text files include differential skipped exon splicing analysis for each knockdown (SE.MATS.JC.txt from rMATS)

extract_protocol:
RNA was harvested using DirectZol RNA Miniprep Kit (Zymo)
RNA libraries for RNA-seq were prepared using Stranded mRNA Prep, Ligation kit (Illumina) following manufacturer's protocols.

treatment_protocol: Knockdown was performed through lentiviral transduction of target-specific shRNAs followed by puromycin selection

tag: genotype


title: L140P-PUF60 rep2

description:
L140PvsWT.SE.MATS.JC.txt
L140P_featurecounts_unfiltered.csv
shi1_L140P_plusD_2


title: L140P-PUF60 rep3

description:
L140PvsWT.SE.MATS.JC.txt
L140P_featurecounts_unfiltered.csv
shi1_L140P_plusD_3


title: JM209B,tumor

description:
Library name: Sample_JM209B
Column name in counts.txt: JM209B


title: WT-PUF60 rep4

description:
L140PvsWT.SE.MATS.JC.txt
L140P_featurecounts_unfiltered.csv
shi1_WT_plusD_4


title: L140P-PUF60 rep1

description:
L140PvsWT.SE.MATS.JC.txt
L140P_featurecounts_unfiltered.csv
shi1_L140P_plusD_1


title: Crosslinked MDAMB231, V5 IN, rep1

description: V5-26-02-2024-18-43-43.reproducible_enriched_windows.tsv.gz

data_processing:
Data was processed using Skipper, available at: http://github.com/yeolab/skipper
Reads were trimmed for adapter sequences and barcode sequences (eCLIP samples) using skewer.
Unique Molecular Identifiers (UMIs) were extracted from raw sequencing reads with fastp
Extracted reads were aligned using STAR: --alignEndsType EndToEnd --genomeDir {params.star_sjdb} --genomeLoad NoSharedMemory --outBAMcompression 10 --outFileNamePrefix {params.outprefix} --winAnchorMultimapNmax 100 --outFilterMultimapNmax 100 --outFilterMultimapScoreRange 1 --outSAMmultNmax 1 --outMultimapperOrder Random --outFilterScoreMin 10 --outFilterType BySJout --limitOutSJcollapsed 5000000 --outReadsUnmapped None --outSAMattrRGline ID:{wildcards.replicate_label} --outSAMattributes All --outSAMmode Full --outSAMtype BAM Unsorted --outSAMunmapped Within --readFilesCommand zcat --outStd Log --readFilesIn {input.fq} --runMode alignReads --runThreadN {threads}
Custom scripts called reproducible enriched windows and repetitive elements as part of Skipper
Assembly: GRCh38
Supplementary files format and content: reproducible enriched window files contain p values, q values, enrichment, and annotations for significantly bound transcriptome tiled windows

extract_protocol:
Cells were lysed and and sonicated in eCLIP lysis buffer, and RNA was fragmented using RNase I (Ambion) and complexes were immunoprecipitated with protein-specific antibodies from Bethyl Laboratories
RNA was reverse transcripted with Superscript III, and adaptor was ligated with T4 RNA Ligase 1, then libraries were constructed with Q5 High-Fidelity 2x Master Mix (NEB)

tag: fraction

value: V5 IN


title: JM229B,tumor

description:
Library name: Sample_JM229B
Column name in counts.txt: JM229B


title: Crosslinked MDAMB436, PUF60 IN, rep2

description: PUF60-MDAMB436.reproducible_enriched_windows.tsv.gz

value: PUF60 IN


title: Crosslinked MDAMB436, PUF60 IN, rep1


title: Crosslinked SUM149, PUF60 IN, rep1

description: PUF60-SUM149.reproducible_enriched_windows.tsv.gz


title: Crosslinked MDAMB231, V5 IN, rep2


title: Crosslinked MDAMB231, PUF60 IN, rep1

description: PUF60-MDAMB231.reproducible_enriched_windows.tsv.gz


title: Crosslinked MDAMB231, PUF60 IN, rep2


title: Crosslinked MCF10A, PUF60 IN, rep2

description: PUF60-MCF10A.reproducible_enriched_windows.tsv.gz


title: Crosslinked MDAMB231, V5 IP, rep2

value: V5 IP


title: Crosslinked MDAMB231, V5 IP, rep1


title: JM219B,tumor

description:
Library name: Sample_JM219B
Column name in counts.txt: JM219B


title: Crosslinked MDAMB231, L140P-PUF60-V5 IN, rep1

description: WT-PUF60-26-02-2024-18-43-43.reproducible_enriched_windows.tsv.gz

value: L140P-PUF60-V5 IN


title: JM217B,tumor

description:
Library name: Sample_JM217B
Column name in counts.txt: JM217B


title: Crosslinked MDAMB231, L140P-PUF60-V5 IP, rep2

value: L140P-PUF60-V5 IP


title: Crosslinked SUM149, PUF60 IN, rep2


title: Crosslinked MDAMB231, L140P-PUF60-V5 IP, rep1


title: Crosslinked MCF10A, PUF60 IN, rep1


title: Crosslinked SUM149, PUF60 IP, rep2

value: PUF60 IP


title: Crosslinked MDAMB231, WT-PUF60-V5 IP, rep1

description: L140P-PUF60-26-02-2024-18-43-43.reproducible_enriched_windows.tsv.gz

value: WT-PUF60-V5 IP


title: Crosslinked SUM149, PUF60 IP, rep1


title: Crosslinked MDAMB231, WT-PUF60-V5 IP, rep2


title: Crosslinked MDAMB436, PUF60 IP, rep2


title: Crosslinked MDAMB436, PUF60 IP, rep1


title: Crosslinked MDAMB231, L140P-PUF60-V5 IN, rep2


title: Crosslinked MDAMB231, PUF60 IP, rep2


title: Crosslinked MDAMB231, PUF60 IP, rep1


title: WT-PUF60 rep2

description:
L140PvsWT.SE.MATS.JC.txt
L140P_featurecounts_unfiltered.csv
shi1_WT_plusD_2


title: WT-PUF60 rep3

description:
L140PvsWT.SE.MATS.JC.txt
L140P_featurecounts_unfiltered.csv
shi1_WT_plusD_3


title: Crosslinked MCF10A, PUF60 IP, rep1


title: SUM149 shPUF60 2 rep3

description:
sum149_puf60.SE.MATS.JC.txt
PUF60_featurecounts_unfiltered.csv
149_PUF60_93-3

value: PUF60 2


title: SUM149 shPUF60 2 rep2

description:
sum149_puf60.SE.MATS.JC.txt
PUF60_featurecounts_unfiltered.csv
149_PUF60_93-2


title: SUM149 shNT rep3

description:
sum149_puf60.SE.MATS.JC.txt
PUF60_featurecounts_unfiltered.csv
149_NT-3

value: NT


title: SUM149 shPUF60 1 rep1

description:
sum149_puf60.SE.MATS.JC.txt
PUF60_featurecounts_unfiltered.csv
149_PUF60_48-1

value: PUF60 1


title: SUM149 shPUF60 1 rep3

description:
sum149_puf60.SE.MATS.JC.txt
PUF60_featurecounts_unfiltered.csv
149_PUF60_48-3


title: SUM149 shNT rep2

description:
sum149_puf60.SE.MATS.JC.txt
PUF60_featurecounts_unfiltered.csv
149_NT-2


title: SUM149 shPUF60 1 rep2

description:
sum149_puf60.SE.MATS.JC.txt
PUF60_featurecounts_unfiltered.csv
149_PUF60_48-2


title: SUM149 shNT rep1

description:
sum149_puf60.SE.MATS.JC.txt
PUF60_featurecounts_unfiltered.csv
149_NT-1


title: MDAMB436 shPUF60 2 rep1

description:
mdamb436_puf60.SE.MATS.JC.txt
PUF60_featurecounts_unfiltered.csv
436_PUF60_93-1


title: MDAMB436 shPUF60 2 rep2

description:
mdamb436_puf60.SE.MATS.JC.txt
PUF60_featurecounts_unfiltered.csv
436_PUF60_93-2


title: MDAMB436 shPUF60 1 rep3

description:
mdamb436_puf60.SE.MATS.JC.txt
PUF60_featurecounts_unfiltered.csv
436_PUF60_48-3


title: MDAMB436 shPUF60 1 rep1

description:
mdamb436_puf60.SE.MATS.JC.txt
PUF60_featurecounts_unfiltered.csv
436_PUF60_48-1


title: MDAMB436 shPUF60 1 rep2

description:
mdamb436_puf60.SE.MATS.JC.txt
PUF60_featurecounts_unfiltered.csv
436_PUF60_48-2


title: MDAMB436 shNT rep3

description:
mdamb436_puf60.SE.MATS.JC.txt
PUF60_featurecounts_unfiltered.csv
436_NT-3


title: Crosslinked MCF10A, PUF60 IP, rep2

description: PUF60-MCF10A.reproducible_enriched_windows.tsv.gz


title: MDAMB231 shPUF60 2 rep3

description:
mdamb231_puf60.SE.MATS.JC.txt
PUF60_featurecounts_unfiltered.csv
231_PUF60_93-3


title: MDAMB231 shPUF60 2 rep2

description:
mdamb231_puf60.SE.MATS.JC.txt
PUF60_featurecounts_unfiltered.csv
231_PUF60_93-2


title: MDAMB231 shPUF60 2 rep1

description:
mdamb231_puf60.SE.MATS.JC.txt
PUF60_featurecounts_unfiltered.csv
231_PUF60_93-1


title: MDAMB436 shNT rep2

description:
mdamb436_puf60.SE.MATS.JC.txt
PUF60_featurecounts_unfiltered.csv
436_NT-2


title: MDAMB436 shNT rep1

description:
mdamb436_puf60.SE.MATS.JC.txt
PUF60_featurecounts_unfiltered.csv
436_NT-1


title: MDAMB231 shPUF60 1 rep1

description:
mdamb231_puf60.SE.MATS.JC.txt
PUF60_featurecounts_unfiltered.csv
231_PUF60_48-1


title: MDAMB231 shPUF60 1 rep3

description:
mdamb231_puf60.SE.MATS.JC.txt
PUF60_featurecounts_unfiltered.csv
231_PUF60_48-3


title: SUM149 shPUF60 2 rep1

description:
sum149_puf60.SE.MATS.JC.txt
PUF60_featurecounts_unfiltered.csv
149_PUF60_93-1


title: MDAMB231 shNT rep1

description:
mdamb231_puf60.SE.MATS.JC.txt
PUF60_featurecounts_unfiltered.csv
231_NT-1


title: MDAMB231 shNT rep3

description:
mdamb231_puf60.SE.MATS.JC.txt
PUF60_featurecounts_unfiltered.csv
231_NT-3


title: MCF10A shPUF60 2 rep1

description:
mcf10a_puf60.SE.MATS.JC.txt
PUF60_featurecounts_unfiltered.csv
10A_PUF60_93-1


title: MCF10A shPUF60 1 rep1

description:
mcf10a_puf60.SE.MATS.JC.txt
PUF60_featurecounts_unfiltered.csv
10A_PUF60_48-1


title: MCF10A shPUF60 1 rep2

description:
mcf10a_puf60.SE.MATS.JC.txt
PUF60_featurecounts_unfiltered.csv
10A_PUF60_48-2


title: MCF10A shPUF60 2 rep2

description:
mcf10a_puf60.SE.MATS.JC.txt
PUF60_featurecounts_unfiltered.csv
10A_PUF60_93-2


title: MCF10A shNT rep2

description:
mcf10a_puf60.SE.MATS.JC.txt
PUF60_featurecounts_unfiltered.csv
10A_NT-2


title: MCF10A shNT rep1

description:
mcf10a_puf60.SE.MATS.JC.txt
PUF60_featurecounts_unfiltered.csv
10A_NT-1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L20 24 nt Rep2

city: Hangzhou

first: Lijia

last: Ma

state: Zhejiang

address: Shilongshan Road No.18, Cloud Town, Xihu District

department: School of Life Sciences

institute: Westlake university

data_processing:
The Amplicon-Seq data was analyzed using CRISPResso 2.13 (--max_paired_end_reads_overlap 140 --min_paired_end_reads_overlap 10 --exclude_bp_from_left 0 --exclude_bp_from_right 0 --plot_window_size 40 --min_frequency_alleles_around_cut_to_plot 0.1)
Assembly: designed sgRNA library
Supplementary files format and content: The xls file, indel frequency in each experiment.
Library strategy: Amplicon-Seq

source_name: HEK293T cell

extract_protocol:
The gDNA was extracted using the Blood/Cells gDNA extraction kit from TIANGEN (TIANGEN DP304-03).
The target sequences were enriched from the gDNA by two rounds of PCR amplification.

value: HEK293T cell


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L11 23 nt Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L20 22 nt Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L20 23 nt Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L20 21 nt G Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L11 22 nt Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L20 20 nt Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L20 21 nt Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L19 24 nt Rep2


title: MDAMB231 shNT rep2

description:
mdamb231_puf60.SE.MATS.JC.txt
PUF60_featurecounts_unfiltered.csv
231_NT-2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L19 23 nt Rep2


title: MDAMB231 shPUF60 1 rep2

description:
mdamb231_puf60.SE.MATS.JC.txt
PUF60_featurecounts_unfiltered.csv
231_PUF60_48-2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L19 20 nt Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L11 21 nt G-HH Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L19 21 nt G Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L18 23 nt Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L18 22 nt Rep2


title: WT-PUF60 rep1

description:
L140PvsWT.SE.MATS.JC.txt
L140P_featurecounts_unfiltered.csv
shi1_WT_plusD_1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L18 24 nt Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L17 24 nt Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L18 21 nt T Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L18 21 nt Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L18 20 nt Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L11 21 nt G Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L11 22 nt-HH Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L17 23 nt Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L17 22 nt Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L17 21 nt Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L16 24 nt Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L16 22 nt Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L17 20 nt Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L17 21 nt G Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L16 21 nt G Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L19 22 nt Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L16 21 nt Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L16 20 nt Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L19 21 nt Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L15 24 nt Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L15 23 nt Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L15 21 nt Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L15 22 nt Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L15 21 nt T Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L11 21 nt-HH Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L15 20 nt Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L14 24 nt Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L14 23 nt Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L14 22 nt Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L14 21 nt Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L14 21 nt T Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L11 21 nt Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L13 24 nt Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L13 21 nt G Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L16 23 nt Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L13 22 nt Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L13 20 nt Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L13 23 nt Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L12 24 nt Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L12 23 nt Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L13 21 nt Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L11 20 nt-HH Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L12 23 nt-HH Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L12 21 nt G Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L12 22 nt Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L12 21 nt G-HH Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L12 21 nt Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L12 22 nt-HH Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L12 21 nt-HH Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L12 20 nt-HH Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L11 23 nt-HH Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L14 20 nt Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L11 24 nt Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L11 20 nt Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L12 20 nt Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L11 22 nt-HH Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L20 24 nt Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L11 22 nt Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L20 23 nt Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L20 21 nt Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L20 20 nt Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L20 21 nt G Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L19 22 nt Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L19 23 nt Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L20 22 nt Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L19 24 nt Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L11 23 nt Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L19 21 nt Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L11 21 nt G-HH Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L19 21 nt G Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L18 23 nt Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L19 20 nt Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L18 21 nt T Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L17 24 nt Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L18 22 nt Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L17 23 nt Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L18 21 nt Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L17 22 nt Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L17 21 nt G Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L17 21 nt Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L16 24 nt Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L17 20 nt Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L16 22 nt Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L15 24 nt Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L11 21 nt-HH Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L16 21 nt G Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L16 21 nt Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L16 20 nt Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L18 24 nt Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L15 23 nt Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L15 21 nt T Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L15 22 nt Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L14 23 nt Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L14 24 nt Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L15 21 nt Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L15 20 nt Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L14 22 nt Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L14 21 nt T Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L18 20 nt Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L14 21 nt Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L11 21 nt G Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L13 24 nt Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L13 23 nt Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L13 22 nt Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L16 23 nt Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L14 20 nt Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L13 21 nt G Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L13 21 nt Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L13 20 nt Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L12 24 nt Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L12 23 nt-HH Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L12 23 nt Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L11 20 nt-HH Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L12 22 nt Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L11 21 nt Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L12 21 nt G-HH Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L12 21 nt Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L12 21 nt-HH Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L12 20 nt Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L12 20 nt-HH Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L11 24 nt Rep1


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L11 20 nt Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L8 24 nt Rep3

source_name: K562-SuperFi Cas9 cell

value: K562-SuperFi Cas9 cell


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L11 23 nt-HH Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L9 23 nt Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L8 23 nt Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L7 23 nt Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L8 24 nt Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L12 22 nt-HH Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L8 24 nt Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L7 24 nt Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L7 24 nt Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L7 23 nt Rep2


title: SuperFi plasmid lipo 3000 transfection at L11-L20 sites- L12 21 nt G Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L7 24 nt Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L7 23 nt Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L6 24 nt Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L6 23 nt Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L9 24 nt Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L6 24 nt Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L6 23 nt Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L5 24 nt Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L6 24 nt Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L6 23 nt Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L5 23 nt Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L9 23 nt Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L5 23 nt Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L5 24 nt Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L4 23 nt Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L5 24 nt Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L4 23 nt Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L4 24 nt Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L8 23 nt Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L5 23 nt Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L4 24 nt Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L8 23 nt Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L3 23 nt Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L3 24 nt Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L3 23 nt Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L4 23 nt Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L3 23 nt Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L2 24 nt Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L3 24 nt Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L2 24 nt Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L3 24 nt Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L2 23 nt Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L2 24 nt Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L2 23 nt Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L1 24 nt Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L1 23 nt Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L10 23 nt Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L4 24 nt Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L1 23 nt Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L1 24 nt Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L10 24 nt Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L10 23 nt Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L10 23 nt Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L9 23 nt Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L9 24 nt Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L9 24 nt Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L3 20 nt Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L10 24 nt Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L2 23 nt Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L2 21 nt T Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L2 22 nt Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L2 21 nt G Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L1 24 nt Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L1 21 nt T Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L2 20 nt Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L1 23 nt Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L9 21 nt G Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L10 21 nt G Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L10 20 nt Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L9 21 nt T Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L10 24 nt Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L10 21 nt T Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L9 20 nt Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L8 21 nt A Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L8 21 nt G Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L1 21 nt G Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L7 20 nt Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L8 20 nt Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L7 21 nt A Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L7 21 nt G Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L6 20 nt Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L1 22 nt Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L6 22 nt Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L5 21 nt A Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L5 22 nt Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L5 21 nt G Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L5 20 nt Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L4 22 nt Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L4 21 nt A Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L4 21 nt G Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L4 20 nt Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L3 21 nt T Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L3 21 nt G Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L3 20 nt Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L3 22 nt Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L2 21 nt G Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L1 20 nt Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L10 22 nt Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L2 21 nt T Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L6 21 nt G Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L2 22 nt Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L2 20 nt Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L6 21 nt T Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L8 22 nt Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L7 22 nt Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L9 22 nt Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L9 22 nt Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L8 22 nt Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L10 21 nt T Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L9 22 nt Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L8 22 nt Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L7 22 nt Rep3


title: SuperFi plasmid electroporation at L1-L10 sites- L10 21 nt G Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L9 20 nt Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L9 21 nt T Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L9 21 nt G Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L10 20 nt Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L10 22 nt Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L8 21 nt A Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L1 21 nt G Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L10 22 nt Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L8 21 nt G Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L1 21 nt T Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L8 20 nt Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L7 20 nt Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L7 21 nt G Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L6 21 nt G Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L7 21 nt A Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L6 21 nt T Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L5 21 nt A Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L6 22 nt Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L6 20 nt Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L4 21 nt A Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L5 22 nt Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L7 22 nt Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L4 21 nt G Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L1 22 nt Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L3 21 nt T Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L5 21 nt G Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L4 22 nt Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L4 20 nt Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L1 20 nt Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L3 22 nt Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L3 21 nt G Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L2 20 nt Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L2 21 nt T Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L1 21 nt T Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L2 22 nt Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L2 21 nt G Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L10 21 nt T Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L10 21 nt G Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L9 20 nt Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L10 20 nt Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L1 21 nt G Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L8 21 nt A Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L8 20 nt Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L9 21 nt T Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L7 21 nt A Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L7 21 nt G Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L7 20 nt Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L5 20 nt Rep2


title: SuperFi plasmid electroporation at L1-L10 sites- L6 21 nt T Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L6 22 nt Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L8 21 nt G Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L5 21 nt A Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L6 20 nt Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L6 21 nt G Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L3 20 nt Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L1 22 nt Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L5 22 nt Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L4 21 nt A Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L5 20 nt Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L4 20 nt Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L3 21 nt G Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L3 21 nt T Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L4 21 nt G Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L9 21 nt G Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L4 22 nt Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L3 22 nt Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L1 20 nt Rep1


title: Control SuperFi plasmid lipo 3000 transfection at L9 site- L9 20 nt Rep3


title: Control SpCas9 plasmid lipo 3000 transfection at L9 site- L9 22 nt Rep2


title: Control SpCas9 plasmid lipo 3000 transfection at L9 site- L9 22 nt Rep1


title: Control SpCas9 plasmid lipo 3000 transfection at L9 site- L9 22 nt Rep3


title: SuperFi Del plasmid lipo 3000 transfection at L9 site- L9 22 nt Rep2


title: SuperFi 951A plasmid lipo 3000 transfection at L9 site- L9 22 nt Rep3


title: SuperFi Del plasmid lipo 3000 transfection at L9 site- L9 22 nt Rep1


title: SuperFi 951A plasmid lipo 3000 transfection at L9 site- L9 22 nt Rep2


title: SuperFi 951A plasmid lipo 3000 transfection at L9 site- L9 20 nt Rep3


title: Control SpCas9 plasmid lipo 3000 transfection at L9 site- L9 20 nt Rep3


title: SuperFi Del plasmid lipo 3000 transfection at L9 site- L9 20 nt Rep1


title: SuperFi Del plasmid lipo 3000 transfection at L9 site- L9 20 nt Rep2


title: SuperFi 951A plasmid lipo 3000 transfection at L9 site- L9 22 nt Rep1


title: SuperFi plasmid electroporation at L1-L10 sites- L5 21 nt G Rep1


title: SuperFi 948A plasmid lipo 3000 transfection at L9 site- L9 22 nt Rep3


title: SuperFi 951A plasmid lipo 3000 transfection at L9 site- L9 20 nt Rep2


title: SuperFi 948A plasmid lipo 3000 transfection at L9 site- L9 22 nt Rep2


title: Control SuperFi plasmid lipo 3000 transfection at L9 site- L9 20 nt Rep2


title: SuperFi Del plasmid lipo 3000 transfection at L9 site- L9 20 nt Rep3


title: SuperFi 948A plasmid lipo 3000 transfection at L9 site- L9 22 nt Rep1


title: SuperFi 948A plasmid lipo 3000 transfection at L9 site- L9 20 nt Rep2


title: SuperFi 948A plasmid lipo 3000 transfection at L9 site- L9 20 nt Rep3


title: Control SpCas9 plasmid lipo 3000 transfection at L9 site- L9 20 nt Rep2


title: SuperFi 948A plasmid lipo 3000 transfection at L9 site- L9 20 nt Rep1


title: SuperFi 929A plasmid lipo 3000 transfection at L9 site- L9 22 nt Rep2


title: SuperFi Del plasmid lipo 3000 transfection at L9 site- L9 22 nt Rep3


title: SuperFi 929A plasmid lipo 3000 transfection at L9 site- L9 22 nt Rep1


title: Control SuperFi plasmid lipo 3000 transfection at L9 site- L9 20 nt Rep1


title: SuperFi 929A plasmid lipo 3000 transfection at L9 site- L9 22 nt Rep3


title: SuperFi 929A plasmid lipo 3000 transfection at L9 site- L9 20 nt Rep2


title: SuperFi 929A plasmid lipo 3000 transfection at L9 site- L9 20 nt Rep3


title: SuperFi 929A plasmid lipo 3000 transfection at L9 site- L9 20 nt Rep1


title: Control SuperFi plasmid lipo 3000 transfection at L9 site- L9 22 nt Rep2


title: SuperFi RNP Dual Mismatched 22 nt gRNA electroporation at L5 site- L5 22MM1 22 nt Rep2


title: SuperFi RNP Dual Mismatched 22 nt gRNA electroporation at L5 site- L5 22MM1 22 nt Rep3


title: SuperFi 951A plasmid lipo 3000 transfection at L9 site- L9 20 nt Rep1


title: Control SuperFi plasmid lipo 3000 transfection at L9 site- L9 22 nt Rep1


title: Control SuperFi RNP electroporation at L5 site- L5 24 nt Rep2


title: Control SuperFi RNP electroporation at L5 site- L5 24 nt Rep1


title: Control SuperFi RNP electroporation at L5 site- L5 22 nt Rep3


title: SuperFi RNP Dual Mismatched 22 nt gRNA electroporation at L5 site- L5 22MM1 22 nt Rep1


title: SuperFi RNP Dual Mismatched 23 nt gRNA electroporation at L9 site- L9 23MM1 23 nt Rep2


title: SuperFi RNP Dual Mismatched 23 nt gRNA electroporation at L9 site- L9 23MM1 23 nt Rep1


title: SuperFi RNP Dual Mismatched 22 nt gRNA electroporation at L9 site- L9 22MM1 22 nt Rep3


title: SuperFi RNP Dual Mismatched 23 nt gRNA electroporation at L9 site- L9 23MM1 23 nt Rep3


title: Control SuperFi RNP electroporation at L9 site- L9 23 nt Rep1


title: Control SuperFi RNP electroporation at L9 site- L9 23 nt Rep2


title: SuperFi RNP Dual Mismatched 22 nt gRNA electroporation at L9 site- L9 22MM1 22 nt Rep1


title: Control SuperFi RNP electroporation at L9 site- L9 23 nt Rep3


title: Control SuperFi RNP electroporation at L9 site- L9 22 nt Rep3


title: Control SuperFi RNP electroporation at L9 site- L9 22 nt Rep2


title: Control SuperFi RNP electroporation at L5 site- L5 22 nt Rep2


title: Control SuperFi RNP electroporation at L9 site- L9 22 nt Rep1


title: SuperFi RNP Dual Mismatched 24 nt gRNA electroporation at L5 site- L5 24MM2 24 nt Rep3


title: SuperFi RNP Dual Mismatched 24 nt gRNA electroporation at L5 site- L5 24MM2 24 nt Rep2


title: SuperFi RNP Dual Mismatched 24 nt gRNA electroporation at L5 site- L5 24MM2 24 nt Rep1


title: SuperFi RNP Dual Mismatched 24 nt gRNA electroporation at L5 site- L5 24MM1 24 nt Rep3


title: Control SuperFi RNP electroporation at L5 site- L5 24 nt Rep3


title: Control SpCas9 plasmid lipo 3000 transfection at L9 site- L9 20 nt Rep1


title: SuperFi RNP Dual Mismatched 22 nt gRNA electroporation at L5 site- L5 22MM2 22 nt Rep3


title: SuperFi RNP Dual Mismatched 22 nt gRNA electroporation at L5 site- L5 22MM2 22 nt Rep2


title: SuperFi RNP Dual Mismatched 22 nt gRNA electroporation at L5 site- L5 22MM2 22 nt Rep1


title: Control SuperFi RNP electroporation at L5 site- L5 22 nt Rep1


title: SuperFi RNP Dual Mismatched 24 nt gRNA electroporation at L5 site- L5 24MM1 24 nt Rep1


title: SuperFi RNP Dual Mismatched 24 nt gRNA electroporation at L5 site- L5 24MM1 24 nt Rep2


title: SuperFi CBE plasmid lipo 3000 transfection at RNF2 site- RNF2 22 nt Rep2


title: SuperFi CBE plasmid lipo 3000 transfection at RNF2 site- RNF2 21 nt Rep3


title: SuperFi CBE plasmid lipo 3000 transfection at RNF2 site- RNF2 22 nt Rep3


title: Control CBE plasmid lipo 3000 transfection at HEK3-2 site- HEK3-2 20 nt Rep3


title: SuperFi CBE plasmid lipo 3000 transfection at RNF2 site- RNF2 22 nt Rep1


title: Control CBE plasmid lipo 3000 transfection at L18 site- L18 20 nt Rep2


title: Control CBE plasmid lipo 3000 transfection at L18 site- L18 20 nt Rep3


title: SuperFi RNP Dual Mismatched 22 nt gRNA electroporation at L9 site- L9 22MM1 22 nt Rep2


title: Control CBE plasmid lipo 3000 transfection at L18 site- L18 20 nt Rep1


title: Control CBE plasmid lipo 3000 transfection at HEK3-2 site- HEK3-2 20 nt Rep2


title: SuperFi CBE plasmid lipo 3000 transfection at RNF2 site- RNF2 21 nt Rep2


title: Control CBE plasmid lipo 3000 transfection at RNF2 site- RNF2 20 nt Rep2


title: Control CBE plasmid lipo 3000 transfection at RNF2 site- RNF2 20 nt Rep3


title: Control CBE plasmid lipo 3000 transfection at RNF2 site- RNF2 20 nt Rep1


title: Control CBE plasmid lipo 3000 transfection at HEK3-2 site- HEK3-2 20 nt Rep1


title: SuperFi CBE plasmid lipo 3000 transfection at L18 site- L18 24 nt Rep3


title: SuperFi CBE plasmid lipo 3000 transfection at L18 site- L18 24 nt Rep1


title: SuperFi CBE plasmid lipo 3000 transfection at L18 site- L18 23 nt Rep3


title: SuperFi CBE plasmid lipo 3000 transfection at L18 site- L18 24 nt Rep2


title: SuperFi CBE plasmid lipo 3000 transfection at L18 site- L18 23 nt Rep2


title: SuperFi CBE plasmid lipo 3000 transfection at L18 site- L18 23 nt Rep1


title: SuperFi CBE plasmid lipo 3000 transfection at L18 site- L18 22 nt Rep3


title: SuperFi CBE plasmid lipo 3000 transfection at RNF2 site- RNF2 21 nt Rep1


title: Control SuperFi plasmid lipo 3000 transfection at L9 site- L9 22 nt Rep3


title: SuperFi CBE plasmid lipo 3000 transfection at L18 site- L18 22 nt Rep2


title: SuperFi CBE plasmid lipo 3000 transfection at L18 site- L18 21 nt Rep1

source_name: K562 cell

value: K562 cell


title: SuperFi CBE plasmid lipo 3000 transfection at L18 site- L18 20 nt Rep2


title: SuperFi CBE plasmid lipo 3000 transfection at L18 site- L18 20 nt Rep3


title: SuperFi CBE plasmid lipo 3000 transfection at L18 site- L18 21 nt Rep3


title: SuperFi CBE plasmid lipo 3000 transfection at HEK3-2 site- HEK3-2 24 nt Rep1


title: SuperFi CBE plasmid lipo 3000 transfection at L18 site- L18 20 nt Rep1


title: SuperFi CBE plasmid lipo 3000 transfection at HEK3-2 site- HEK3-2 23 nt Rep2


title: SuperFi CBE plasmid lipo 3000 transfection at L18 site- L18 21 nt Rep2


title: SuperFi CBE plasmid lipo 3000 transfection at HEK3-2 site- HEK3-2 23 nt Rep1


title: SuperFi CBE plasmid lipo 3000 transfection at RNF2 site- RNF2 20 nt Rep3


title: SuperFi CBE plasmid lipo 3000 transfection at HEK3-2 site- HEK3-2 24 nt Rep3


title: SuperFi CBE plasmid lipo 3000 transfection at HEK3-2 site- HEK3-2 22 nt Rep2


title: SuperFi CBE plasmid lipo 3000 transfection at HEK3-2 site- HEK3-2 21 nt Rep3


title: SuperFi CBE plasmid lipo 3000 transfection at HEK3-2 site- HEK3-2 24 nt Rep2


title: SuperFi CBE plasmid lipo 3000 transfection at RNF2 site- RNF2 20 nt Rep2


title: SuperFi CBE plasmid lipo 3000 transfection at HEK3-2 site- HEK3-2 22 nt Rep1


title: SuperFi CBE plasmid lipo 3000 transfection at HEK3-2 site- HEK3-2 20 nt Rep2


title: SuperFi CBE plasmid lipo 3000 transfection at HEK3-2 site- HEK3-2 20 nt Rep3


title: SuperFi CBE plasmid lipo 3000 transfection at HEK3-2 site- HEK3-2 20 nt Rep1


title: SuperFi CBE plasmid lipo 3000 transfection at HEK3-2 site- HEK3-2 21 nt Rep1


title: SuperFi CBE plasmid lipo 3000 transfection at RNF2 site- RNF2 24 nt Rep2


title: SuperFi CBE plasmid lipo 3000 transfection at RNF2 site- RNF2 24 nt Rep3


title: SuperFi CBE plasmid lipo 3000 transfection at L18 site- L18 22 nt Rep1


title: SuperFi CBE plasmid lipo 3000 transfection at RNF2 site- RNF2 24 nt Rep1


title: SuperFi CBE plasmid lipo 3000 transfection at RNF2 site- RNF2 23 nt Rep1


title: SuperFi CBE plasmid lipo 3000 transfection at RNF2 site- RNF2 20 nt Rep1


title: SuperFi ABE plasmid lipo 3000 transfection at HEK1 site- HEK1 21 nt G Rep3


title: SuperFi CBE plasmid lipo 3000 transfection at RNF2 site- RNF2 23 nt Rep3


title: SuperFi CBE plasmid lipo 3000 transfection at HEK3-2 site- HEK3-2 23 nt Rep3


title: SuperFi ABE plasmid lipo 3000 transfection at HEK1 site- HEK1 21 nt G Rep2


title: SuperFi ABE plasmid lipo 3000 transfection at HEK1 site- HEK1 21 nt T Rep3


title: SuperFi ABE plasmid lipo 3000 transfection at HEK1 site- HEK1 21 nt T Rep2


title: Control CBE plasmid lipo 3000 transfection at L2 site- L2 20 nt Rep3


title: SuperFi CBE plasmid lipo 3000 transfection at HEK3-2 site- HEK3-2 21 nt Rep2


title: Control CBE plasmid lipo 3000 transfection at L2 site- L2 20 nt Rep2


title: SuperFi ABE plasmid lipo 3000 transfection at HEK1 site- HEK1 21 nt T Rep1


title: SuperFi CBE plasmid lipo 3000 transfection at HEK3-2 site- HEK3-2 22 nt Rep3


title: SuperFi ABE plasmid lipo 3000 transfection at HEK1 site- HEK1 21 nt G Rep1


title: Control ABE plasmid lipo 3000 transfection at HEK1 site- HEK1 20 nt Rep2


title: Control ABE plasmid lipo 3000 transfection at HEK1 site- HEK1 20 nt Rep3


title: Control CBE plasmid lipo 3000 transfection at L2 site- L2 20 nt Rep1


title: SuperFi CBE plasmid lipo 3000 transfection at L2 site- L2 24 nt Rep3


title: SuperFi CBE plasmid lipo 3000 transfection at L2 site- L2 24 nt Rep2


title: SuperFi CBE plasmid lipo 3000 transfection at L2 site- L2 24 nt Rep1


title: SuperFi CBE plasmid lipo 3000 transfection at L2 site- L2 23 nt Rep1


title: SuperFi CBE plasmid lipo 3000 transfection at L2 site- L2 23 nt Rep2


title: SuperFi ABE plasmid lipo 3000 transfection at HEK1 site- HEK1 20 nt Rep3


title: SuperFi CBE plasmid lipo 3000 transfection at L2 site- L2 22 nt Rep2


title: SuperFi CBE plasmid lipo 3000 transfection at RNF2 site- RNF2 23 nt Rep2


title: SuperFi CBE plasmid lipo 3000 transfection at L2 site- L2 22 nt Rep3


title: SuperFi CBE plasmid lipo 3000 transfection at L2 site- L2 22 nt Rep1


title: SuperFi CBE plasmid lipo 3000 transfection at L2 site- L2 21 nt G Rep3


title: SuperFi CBE plasmid lipo 3000 transfection at L2 site- L2 21 nt G Rep2


title: SuperFi CBE plasmid lipo 3000 transfection at L2 site- L2 21 nt G Rep1


title: SuperFi CBE plasmid lipo 3000 transfection at L2 site- L2 21 nt T Rep2


title: SuperFi CBE plasmid lipo 3000 transfection at L2 site- L2 21 nt T Rep1


title: SuperFi CBE plasmid lipo 3000 transfection at L2 site- L2 20 nt Rep2


title: SuperFi CBE plasmid lipo 3000 transfection at L2 site- L2 20 nt Rep1


title: SuperFi ABE plasmid lipo 3000 transfection at HEK1 site- HEK1 23 nt Rep2


title: SuperFi ABE plasmid lipo 3000 transfection at HEK1 site- HEK1 24 nt Rep1


title: SuperFi ABE plasmid lipo 3000 transfection at HEK1 site- HEK1 23 nt Rep3


title: SuperFi ABE plasmid lipo 3000 transfection at HEK1 site- HEK1 24 nt Rep2


title: Control ABE plasmid lipo 3000 transfection at HEK1 site- HEK1 20 nt Rep1


title: SuperFi ABE plasmid lipo 3000 transfection at HEK1 site- HEK1 24 nt Rep3


title: SuperFi ABE plasmid lipo 3000 transfection at HEK1 site- HEK1 22 nt Rep1


title: SuperFi ABE plasmid lipo 3000 transfection at LIG1 site- LIG1 21 nt G Rep3


title: SuperFi ABE plasmid lipo 3000 transfection at HEK1 site- HEK1 23 nt Rep1


title: SuperFi CBE plasmid lipo 3000 transfection at L2 site- L2 23 nt Rep3


title: SuperFi ABE plasmid lipo 3000 transfection at HEK1 site- HEK1 22 nt Rep2


title: SuperFi ABE plasmid lipo 3000 transfection at HEK1 site- HEK1 22 nt Rep3


title: SuperFi ABE plasmid lipo 3000 transfection at LIG1 site- LIG1 21 nt G Rep1


title: SuperFi ABE plasmid lipo 3000 transfection at LIG1 site- LIG1 21 nt G Rep2


title: Control ABE plasmid lipo 3000 transfection at HEK2 site- HEK2 20 nt Rep3


title: SuperFi ABE plasmid lipo 3000 transfection at LIG1 site- LIG1 21 nt A Rep1


title: SuperFi ABE plasmid lipo 3000 transfection at LIG1 site- LIG1 21 nt A Rep3


title: Control ABE plasmid lipo 3000 transfection at HEK2 site- HEK2 20 nt Rep2


title: Control ABE plasmid lipo 3000 transfection at HEK2 site- HEK2 20 nt Rep1


title: SuperFi CBE plasmid lipo 3000 transfection at L2 site- L2 21 nt T Rep3


title: SuperFi ABE plasmid lipo 3000 transfection at LIG1 site- LIG1 21 nt A Rep2


title: Control ABE plasmid lipo 3000 transfection at LIG1 site- LIG1 20 nt Rep3


title: Control ABE plasmid lipo 3000 transfection at LIG1 site- LIG1 20 nt Rep1


title: SuperFi ABE plasmid lipo 3000 transfection at HEK2 site- HEK2 24 nt Rep3


title: SuperFi CBE plasmid lipo 3000 transfection at L2 site- L2 20 nt Rep3


title: SuperFi ABE plasmid lipo 3000 transfection at HEK1 site- HEK1 20 nt Rep2


title: SuperFi ABE plasmid lipo 3000 transfection at HEK2 site- HEK2 24 nt Rep1


title: SuperFi ABE plasmid lipo 3000 transfection at HEK2 site- HEK2 23 nt Rep2


title: SuperFi ABE plasmid lipo 3000 transfection at LIG1 site- LIG1 20 nt Rep3


title: SuperFi ABE plasmid lipo 3000 transfection at HEK2 site- HEK2 23 nt Rep3


title: SuperFi ABE plasmid lipo 3000 transfection at HEK2 site- HEK2 23 nt Rep1


title: SuperFi ABE plasmid lipo 3000 transfection at HEK1 site- HEK1 20 nt Rep1


title: SuperFi ABE plasmid lipo 3000 transfection at HEK2 site- HEK2 22 nt Rep3


title: SuperFi ABE plasmid lipo 3000 transfection at HEK2 site- HEK2 22 nt Rep2


title: SuperFi ABE plasmid lipo 3000 transfection at HEK2 site- HEK2 20 nt Rep3


title: SuperFi ABE plasmid lipo 3000 transfection at HEK2 site- HEK2 21 nt Rep3


title: SuperFi ABE plasmid lipo 3000 transfection at HEK2 site- HEK2 20 nt Rep2


title: SuperFi ABE plasmid lipo 3000 transfection at HEK2 site- HEK2 21 nt Rep2


title: SuperFi ABE plasmid lipo 3000 transfection at HEK2 site- HEK2 21 nt Rep1


title: SuperFi ABE plasmid lipo 3000 transfection at LIG1 site- LIG1 20 nt Rep2


title: SuperFi ABE plasmid lipo 3000 transfection at HEK2 site- HEK2 20 nt Rep1


title: SuperFi ABE plasmid lipo 3000 transfection at LIG1 site- LIG1 24 nt Rep3


title: SuperFi ABE plasmid lipo 3000 transfection at LIG1 site- LIG1 24 nt Rep1


title: SuperFi ABE plasmid lipo 3000 transfection at LIG1 site- LIG1 24 nt Rep2


title: SuperFi ABE plasmid lipo 3000 transfection at LIG1 site- LIG1 23 nt Rep3


title: SuperFi ABE plasmid lipo 3000 transfection at LIG1 site- LIG1 23 nt Rep2


title: SuperFi ABE plasmid lipo 3000 transfection at LIG1 site- LIG1 23 nt Rep1


title: Control ABE plasmid lipo 3000 transfection at LIG1 site- LIG1 20 nt Rep2


title: SuperFi ABE plasmid lipo 3000 transfection at LIG1 site- LIG1 22 nt Rep1


title: SuperFi ABE plasmid lipo 3000 transfection at L4 site- L4 21 nt A Rep3


title: SuperFi ABE plasmid lipo 3000 transfection at LIG1 site- LIG1 22 nt Rep2


title: SuperFi ABE plasmid lipo 3000 transfection at L4 site- L4 21 nt A Rep2


title: SuperFi ABE plasmid lipo 3000 transfection at LIG1 site- LIG1 20 nt Rep1


title: SuperFi ABE plasmid lipo 3000 transfection at HEK2 site- HEK2 24 nt Rep2


title: SuperFi ABE plasmid lipo 3000 transfection at L4 site- L4 21 nt G Rep3


title: SuperFi ABE plasmid lipo 3000 transfection at L4 site- L4 21 nt G Rep2


title: SuperFi ABE plasmid lipo 3000 transfection at HEK2 site- HEK2 22 nt Rep1


title: SuperFi ABE plasmid lipo 3000 transfection at L4 site- L4 21 nt G Rep1


title: Control ABE plasmid lipo 3000 transfection at L5 site- L5 20 nt Rep1


title: Control ABE plasmid lipo 3000 transfection at L4 site- L4 20 nt Rep3


title: Control ABE plasmid lipo 3000 transfection at L5 site- L5 20 nt Rep2


title: Control ABE plasmid lipo 3000 transfection at L5 site- L5 20 nt Rep3


title: Control ABE plasmid lipo 3000 transfection at L4 site- L4 20 nt Rep1


title: SuperFi ABE plasmid lipo 3000 transfection at L5 site- L5 24 nt Rep3


title: SuperFi ABE plasmid lipo 3000 transfection at L5 site- L5 24 nt Rep2


title: SuperFi ABE plasmid lipo 3000 transfection at L5 site- L5 23 nt Rep3


title: SuperFi ABE plasmid lipo 3000 transfection at L5 site- L5 24 nt Rep1


title: SuperFi ABE plasmid lipo 3000 transfection at L4 site- L4 21 nt A Rep1


title: SuperFi ABE plasmid lipo 3000 transfection at L5 site- L5 23 nt Rep2


title: SuperFi ABE plasmid lipo 3000 transfection at LIG1 site- LIG1 22 nt Rep3


title: SuperFi ABE plasmid lipo 3000 transfection at L5 site- L5 23 nt Rep1


title: SuperFi ABE plasmid lipo 3000 transfection at L4 site- L4 20 nt Rep3


title: SuperFi ABE plasmid lipo 3000 transfection at L5 site- L5 22 nt Rep1


title: SuperFi ABE plasmid lipo 3000 transfection at L5 site- L5 22 nt Rep2


title: SuperFi ABE plasmid lipo 3000 transfection at L5 site- L5 21 nt A Rep2


title: SuperFi ABE plasmid lipo 3000 transfection at L5 site- L5 21 nt A Rep3


title: SuperFi ABE plasmid lipo 3000 transfection at L5 site- L5 21 nt A Rep1


title: SuperFi ABE plasmid lipo 3000 transfection at L5 site- L5 21 nt G Rep3


title: SuperFi ABE plasmid lipo 3000 transfection at L5 site- L5 21 nt G Rep2


title: SuperFi ABE plasmid lipo 3000 transfection at L5 site- L5 20 nt Rep3


title: SuperFi ABE plasmid lipo 3000 transfection at L5 site- L5 21 nt G Rep1


title: SuperFi ABE plasmid lipo 3000 transfection at L5 site- L5 20 nt Rep1


title: SuperFi ABE plasmid lipo 3000 transfection at L5 site- L5 20 nt Rep2


title: SuperFi ABE plasmid lipo 3000 transfection at L4 site- L4 20 nt Rep2


title: SuperFi ABE plasmid lipo 3000 transfection at L4 site- L4 24 nt Rep3


title: SuperFi ABE plasmid lipo 3000 transfection at L4 site- L4 24 nt Rep1


title: Control ABE plasmid lipo 3000 transfection at L4 site- L4 20 nt Rep2


title: SuperFi ABE plasmid lipo 3000 transfection at L4 site- L4 24 nt Rep2


title: SuperFi ABE plasmid lipo 3000 transfection at L4 site- L4 23 nt Rep3


title: SuperFi ABE plasmid lipo 3000 transfection at L4 site- L4 22 nt Rep3


title: SuperFi ABE plasmid lipo 3000 transfection at L4 site- L4 23 nt Rep1


title: SuperFi CBE plasmid lipo 3000 transfection at HEK3 site- HEK3 22 nt Rep3


title: SuperFi ABE plasmid lipo 3000 transfection at L5 site- L5 22 nt Rep3


title: SuperFi ABE plasmid lipo 3000 transfection at L4 site- L4 20 nt Rep1


title: SuperFi ABE plasmid lipo 3000 transfection at L4 site- L4 22 nt Rep2


title: SuperFi CBE plasmid lipo 3000 transfection at HEK3 site- HEK3 22 nt Rep2


title: SuperFi CBE plasmid lipo 3000 transfection at HEK3 site- HEK3 21 nt Rep3


title: SuperFi CBE plasmid lipo 3000 transfection at HEK3 site- HEK3 21 nt Rep2


title: Control CBE plasmid lipo 3000 transfection at L1-2 site- L1-2 20 nt Rep3


title: SuperFi CBE plasmid lipo 3000 transfection at HEK3 site- HEK3 21 nt Rep1


title: Control CBE plasmid lipo 3000 transfection at HEK3 site- HEK3 20 nt Rep1


title: Control CBE plasmid lipo 3000 transfection at HEK3 site- HEK3 20 nt Rep3


title: Control CBE plasmid lipo 3000 transfection at L1-2 site- L1-2 20 nt Rep1


title: Control CBE plasmid lipo 3000 transfection at L1-2 site- L1-2 20 nt Rep2


title: Control CBE plasmid lipo 3000 transfection at HEK3 site- HEK3 20 nt Rep2


title: SuperFi CBE plasmid lipo 3000 transfection at L1-2 site- L1-2 24 nt Rep3


title: SuperFi CBE plasmid lipo 3000 transfection at HEK3 site- HEK3 20 nt Rep3


title: SuperFi CBE plasmid lipo 3000 transfection at L1-2 site- L1-2 24 nt Rep2


title: SuperFi CBE plasmid lipo 3000 transfection at L1-2 site- L1-2 24 nt Rep1


title: SuperFi CBE plasmid lipo 3000 transfection at L1-2 site- L1-2 23 nt Rep1


title: SuperFi CBE plasmid lipo 3000 transfection at L1-2 site- L1-2 22 nt Rep3


title: SuperFi CBE plasmid lipo 3000 transfection at L1-2 site- L1-2 23 nt Rep3


title: SuperFi CBE plasmid lipo 3000 transfection at L1-2 site- L1-2 22 nt Rep2


title: SuperFi ABE plasmid lipo 3000 transfection at L4 site- L4 23 nt Rep2


title: SuperFi CBE plasmid lipo 3000 transfection at L1-2 site- L1-2 23 nt Rep2


title: SuperFi ABE plasmid lipo 3000 transfection at L4 site- L4 22 nt Rep1


title: SuperFi CBE plasmid lipo 3000 transfection at L1-2 site- L1-2 22 nt Rep1


title: SuperFi CBE plasmid lipo 3000 transfection at L1-2 site- L1-2 21 nt Rep3


title: SuperFi CBE plasmid lipo 3000 transfection at L1-2 site- L1-2 21 nt Rep2


title: SuperFi CBE plasmid lipo 3000 transfection at HEK3 site- HEK3 20 nt Rep2


title: SuperFi CBE plasmid lipo 3000 transfection at L1-2 site- L1-2 20 nt Rep2


title: SuperFi CBE plasmid lipo 3000 transfection at L1-2 site- L1-2 21 nt Rep1


title: SuperFi CBE plasmid lipo 3000 transfection at HEK3 site- HEK3 22 nt Rep1


title: SuperFi CBE plasmid lipo 3000 transfection at L1-2 site- L1-2 20 nt Rep3


title: SuperFi CBE plasmid lipo 3000 transfection at HEK3 site- HEK3 24 nt Rep3


title: SuperFi CBE plasmid lipo 3000 transfection at HEK3 site- HEK3 24 nt Rep2


title: SuperFi CBE plasmid lipo 3000 transfection at HEK3 site- HEK3 24 nt Rep1


title: SuperFi CBE plasmid lipo 3000 transfection at HEK3 site- HEK3 23 nt Rep3


title: SuperFi CBE plasmid lipo 3000 transfection at HEK3 site- HEK3 23 nt Rep2


title: SpCas9n PEmax plasmid lipo 3000 transfection at L5 site- L5 20 nt pegRNA Rep3


title: SuperFi CBE plasmid lipo 3000 transfection at HEK3 site- HEK3 20 nt Rep1


title: SuperFi CBE plasmid lipo 3000 transfection at HEK3 site- HEK3 23 nt Rep1


title: SpCas9n PEmax plasmid lipo 3000 transfection at HEK3 site- HEK3 20 nt pegRNA Rep3


title: SuperFi PEmax plasmid lipo 3000 transfection at HEK3 site- HEK3 24 nt pegRNA Rep3


title: SuperFi PEmax plasmid lipo 3000 transfection at L5 site- L5 24 nt pegRNA Rep1


title: SuperFi PEmax plasmid lipo 3000 transfection at HEK3 site- HEK3 22 nt pegRNA Rep3


title: SuperFi PEmax plasmid lipo 3000 transfection at L5 site- L5 23 nt pegRNA Rep3


title: SuperFi PEmax plasmid lipo 3000 transfection at L5 site- L5 23 nt pegRNA Rep1


title: SuperFi PEmax plasmid lipo 3000 transfection at HEK3 site- HEK3 20 nt pegRNA Rep3


title: SuperFi PEmax plasmid lipo 3000 transfection at L5 site- L5 24 nt pegRNA Rep3


title: SuperFi PEmax plasmid lipo 3000 transfection at L5 site- L5 21 nt pegRNA Rep3


title: SuperFi PEmax plasmid lipo 3000 transfection at L5 site- L5 22 nt pegRNA Rep3


title: SuperFi PEmax plasmid lipo 3000 transfection at HEK3 site- HEK3 21 nt pegRNA Rep3


title: SuperFi PEmax plasmid lipo 3000 transfection at L5 site- L5 20 nt pegRNA Rep3


title: SpCas9n PEmax plasmid lipo 3000 transfection at HEK3 site- HEK3 20 nt pegRNA Rep2


title: SpCas9n PEmax plasmid lipo 3000 transfection at L5 site- L5 20 nt pegRNA Rep2


title: SuperFi PEmax plasmid lipo 3000 transfection at HEK3 site- HEK3 23 nt pegRNA Rep2


title: SuperFi CBE plasmid lipo 3000 transfection at L1-2 site- L1-2 20 nt Rep1


title: SuperFi PEmax plasmid lipo 3000 transfection at HEK3 site- HEK3 22 nt pegRNA Rep2


title: SuperFi PEmax plasmid lipo 3000 transfection at L5 site- L5 22 nt pegRNA Rep1


title: SuperFi PEmax plasmid lipo 3000 transfection at HEK3 site- HEK3 21 nt pegRNA Rep2


title: SuperFi PEmax plasmid lipo 3000 transfection at L5 site- L5 23 nt pegRNA Rep2


title: SuperFi PEmax plasmid lipo 3000 transfection at L5 site- L5 22 nt pegRNA Rep2


title: SuperFi PEmax plasmid lipo 3000 transfection at HEK3 site- HEK3 23 nt pegRNA Rep3


title: SuperFi PEmax plasmid lipo 3000 transfection at L5 site- L5 24 nt pegRNA Rep2


title: SuperFi PEmax plasmid lipo 3000 transfection at L5 site- L5 21 nt pegRNA Rep1


title: SuperFi PEmax plasmid lipo 3000 transfection at L5 site- L5 21 nt pegRNA Rep2


title: SpCas9n PEmax plasmid lipo 3000 transfection at L5 site- L5 20 nt pegRNA Rep1


title: SuperFi PEmax plasmid lipo 3000 transfection at L5 site- L5 20 nt pegRNA Rep2


title: SpCas9n PEmax plasmid lipo 3000 transfection at HEK3 site- HEK3 20 nt pegRNA Rep1


title: SuperFi PEmax plasmid lipo 3000 transfection at HEK3 site- HEK3 24 nt pegRNA Rep1


title: SuperFi PEmax plasmid lipo 3000 transfection at HEK3 site- HEK3 22 nt pegRNA Rep1


title: SuperFi PEmax plasmid lipo 3000 transfection at HEK3 site- HEK3 23 nt pegRNA Rep1


title: SuperFi PEmax plasmid lipo 3000 transfection at HEK3 site- HEK3 20 nt pegRNA Rep1


title: SuperFi PEmax plasmid lipo 3000 transfection at HEK3 site- HEK3 21 nt pegRNA Rep1


title: SuperFi PEmax plasmid lipo 3000 transfection at EMX1 site- EMX1 23 nt pegRNA Rep1


title: SuperFi PEmax plasmid lipo 3000 transfection at HEK3 site- HEK3 24 nt pegRNA Rep2


title: SuperFi PEmax plasmid lipo 3000 transfection at EMX1 site- EMX1 22 nt pegRNA Rep1


title: SuperFi PEmax plasmid lipo 3000 transfection at L5 site- L5 20 nt pegRNA Rep1


title: SuperFi PEmax plasmid lipo 3000 transfection at EMX1 site- EMX1 21 nt pegRNA Rep1


title: SuperFi PEmax plasmid lipo 3000 transfection at EMX1 site- EMX1 20 nt pegRNA Rep1


title: SuperFi PEmax plasmid lipo 3000 transfection at EMX1 site- EMX1 23 nt pegRNA Rep3


title: SuperFi PEmax plasmid lipo 3000 transfection at EMX1 site- EMX1 24 nt pegRNA Rep3


title: SuperFi PEmax plasmid lipo 3000 transfection at EMX1 site- EMX1 22 nt pegRNA Rep3


title: SuperFi PEmax plasmid lipo 3000 transfection at EMX1 site- EMX1 24 nt pegRNA Rep2


title: SuperFi PEmax plasmid lipo 3000 transfection at EMX1 site- EMX1 20 nt pegRNA Rep3


title: SuperFi PEmax plasmid lipo 3000 transfection at EMX1 site- EMX1 23 nt pegRNA Rep2


title: SuperFi PEmax plasmid lipo 3000 transfection at EMX1 site- EMX1 21 nt pegRNA Rep3


title: SuperFi PEmax plasmid lipo 3000 transfection at EMX1 site- EMX1 22 nt pegRNA Rep2


title: SuperFi PEmax plasmid lipo 3000 transfection at EMX1 site- EMX1 20 nt pegRNA Rep2


title: SpCas9n PEmax plasmid lipo 3000 transfection at EMX1 site- EMX1 20 nt pegRNA Rep1


title: SuperFi PEmax plasmid lipo 3000 transfection at EMX1 site- EMX1 24 nt pegRNA Rep1


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L5 site- L5 15MM 22 nt Rep2


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L5 site- L5 14MM 22 nt Rep2


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L5 site- L5 13MM 22 nt Rep2


title: SuperFi PEmax plasmid lipo 3000 transfection at HEK3 site- HEK3 20 nt pegRNA Rep2


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L5 site- L5 11MM 22 nt Rep2


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L5 site- L5 10MM 22 nt Rep2


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L5 site- L5 12MM 22 nt Rep2


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L5 site- L5 8MM 22 nt Rep2


title: SpCas9n PEmax plasmid lipo 3000 transfection at EMX1 site- EMX1 20 nt pegRNA Rep3


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L5 site- L5 9MM 22 nt Rep2


title: SuperFi RNP electroporation at L18 site- L18 21 nt T Rep2


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L5 site- L5 6MM 22 nt Rep2


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L5 site- L5 5MM 22 nt Rep2


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L5 site- L5 4MM 22 nt Rep2


title: SpCas9n PEmax plasmid lipo 3000 transfection at EMX1 site- EMX1 20 nt pegRNA Rep2


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L5 site- L5 3MM 22 nt Rep2


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L5 site- L5 2MM 22 nt Rep2


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L5 site- L5 7MM 22 nt Rep2


title: SuperFi RNP electroporation at L5 site- L5 22 nt Rep4


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L5 site- L5 1MM 22 nt Rep2


title: SuperFi RNP electroporation at L18 site- L18 21 nt Rep2


title: SuperFi PEmax plasmid lipo 3000 transfection at EMX1 site- EMX1 21 nt pegRNA Rep2


title: SpCas9 RNP electroporation at L9 site- L9 20 nt Rep3


title: SpCas9 RNP electroporation at L9 site- L9 22 nt Rep4


title: SpCas9 RNP electroporation at L18 site- L18 20 nt Rep3


title: SpCas9 RNP electroporation at L18 site- L18 22 nt Rep3


title: SpCas9 RNP electroporation at L5 site- L5 20 nt Rep3


title: SpCas9 RNP electroporation at L9 site- L9 22 nt Rep2


title: SpCas9 RNP electroporation at L9 site- L9 20 nt Rep1


title: SpCas9 RNP electroporation at L18 site- L18 20 nt Rep2


title: SuperFi RNP electroporation at L5 site- L5 24 nt Rep2


title: SpCas9 RNP electroporation at L18 site- L18 22 nt Rep2


title: SpCas9 RNP electroporation at L5 site- L5 22 nt Rep2


title: SuperFi RNP electroporation at L9 site- L9 24 nt Rep3


title: SuperFi RNP electroporation at L9 site- L9 23 nt Rep3


title: SpCas9 RNP electroporation at L5 site- L5 20 nt Rep2


title: SuperFi RNP electroporation at L9 site- L9 21 nt Rep3


title: SpCas9 RNP electroporation at L5 site- L5 22 nt Rep3


title: SuperFi RNP electroporation at L5 site- L5 23 nt Rep2


title: SuperFi RNP electroporation at L9 site- L9 20 nt Rep3


title: SuperFi RNP electroporation at L9 site- L9 24 nt Rep2


title: SuperFi RNP electroporation at L9 site- L9 20 nt Rep2


title: SuperFi RNP electroporation at L9 site- L9 21 nt Rep2


title: SuperFi RNP electroporation at L9 site- L9 23 nt Rep2


title: SuperFi RNP electroporation at L9 site- L9 22 nt Rep3


title: SpCas9 RNP electroporation at L9 site- L9 20 nt Rep2


title: SuperFi RNP electroporation at L18 site- L18 24 nt Rep3


title: SuperFi RNP electroporation at L18 site- L18 23 nt Rep3


title: SuperFi RNP electroporation at L18 site- L18 20 nt Rep2


title: SuperFi RNP electroporation at L18 site- L18 22 nt Rep3


title: SuperFi RNP electroporation at L18 site- L18 21 nt T Rep3


title: SuperFi RNP electroporation at L18 site- L18 21 nt Rep3


title: SuperFi RNP electroporation at L9 site- L9 22 nt Rep4


title: SuperFi RNP electroporation at L18 site- L18 20 nt Rep3


title: SuperFi RNP electroporation at L5 site- L5 24 nt Rep3


title: SuperFi RNP electroporation at L5 site- L5 22 nt Rep3


title: SuperFi RNP electroporation at L5 site- L5 23 nt Rep3


title: SuperFi RNP electroporation at L5 site- L5 21 nt G Rep2


title: SuperFi RNP electroporation at L5 site- L5 21 nt A Rep2


title: SuperFi RNP electroporation at L5 site- L5 21 nt A Rep3


title: SuperFi RNP electroporation at L5 site- L5 22 nt Rep2


title: SuperFi RNP electroporation at L5 site- L5 20 nt Rep3


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L9 site- L9 20MM 22 nt Rep3


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L9 site- L9 21MM 22 nt Rep3


title: SuperFi RNP electroporation at L5 site- L5 21 nt G Rep3


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L9 site- L9 17MM 22 nt Rep3


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L9 site- L9 16MM 22 nt Rep3


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L9 site- L9 15MM 22 nt Rep3


title: SuperFi RNP electroporation at L9 site- L9 24 nt Rep1


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L9 site- L9 14MM 22 nt Rep3


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L9 site- L9 13MM 22 nt Rep3


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L9 site- L9 11MM 22 nt Rep3


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L9 site- L9 12MM 22 nt Rep3


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L9 site- L9 10MM 22 nt Rep3


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L9 site- L9 18MM 22 nt Rep3


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L9 site- L9 8MM 22 nt Rep3


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L9 site- L9 9MM 22 nt Rep3


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L9 site- L9 7MM 22 nt Rep3


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L9 site- L9 6MM 22 nt Rep3


title: SuperFi RNP electroporation at L9 site- L9 23 nt Rep1


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L9 site- L9 5MM 22 nt Rep3


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L9 site- L9 19MM 22 nt Rep3


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L9 site- L9 21MM 22 nt Rep2


title: SuperFi RNP electroporation at L9 site- L9 22 nt Rep6


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L9 site- L9 2MM 22 nt Rep3


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L9 site- L9 1MM 22 nt Rep3


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L9 site- L9 22MM 22 nt Rep2


title: SuperFi RNP electroporation at L9 site- L9 22 nt Rep2


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L9 site- L9 22MM 22 nt Rep3


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L9 site- L9 20MM 22 nt Rep2


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L9 site- L9 19MM 22 nt Rep2


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L9 site- L9 16MM 22 nt Rep2


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L9 site- L9 17MM 22 nt Rep2


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L9 site- L9 18MM 22 nt Rep2


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L9 site- L9 12MM 22 nt Rep2


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L9 site- L9 15MM 22 nt Rep2


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L9 site- L9 11MM 22 nt Rep2


title: SuperFi RNP electroporation at L9 site- L9 21 nt Rep1


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L9 site- L9 13MM 22 nt Rep2


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L9 site- L9 9MM 22 nt Rep2


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L9 site- L9 7MM 22 nt Rep2


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L9 site- L9 10MM 22 nt Rep2


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L9 site- L9 8MM 22 nt Rep2


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L9 site- L9 6MM 22 nt Rep2


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L9 site- L9 4MM 22 nt Rep3


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L9 site- L9 3MM 22 nt Rep3


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L9 site- L9 5MM 22 nt Rep2


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L9 site- L9 4MM 22 nt Rep2


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L9 site- L9 2MM 22 nt Rep2


title: SuperFi RNP electroporation at L9 site- L9 22 nt Rep5


title: SuperFi RNP electroporation at L9 site- L9 20 nt Rep1


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L9 site- L9 1MM 22 nt Rep2


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L9 site- L9 14MM 22 nt Rep2


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L5 site- L5 19MM 22 nt Rep3


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L5 site- L5 21MM 22 nt Rep3


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L5 site- L5 15MM 22 nt Rep3


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L5 site- L5 18MM 22 nt Rep3


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L5 site- L5 20MM 22 nt Rep3


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L5 site- L5 17MM 22 nt Rep3


title: SuperFi RNP electroporation at L18 site- L18 24 nt Rep2


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L5 site- L5 14MM 22 nt Rep3


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L5 site- L5 13MM 22 nt Rep3


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L5 site- L5 12MM 22 nt Rep3


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L5 site- L5 10MM 22 nt Rep3


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L5 site- L5 9MM 22 nt Rep3


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L5 site- L5 6MM 22 nt Rep3


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L5 site- L5 7MM 22 nt Rep3


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L5 site- L5 8MM 22 nt Rep3


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L9 site- L9 3MM 22 nt Rep2


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L5 site- L5 5MM 22 nt Rep3


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L5 site- L5 4MM 22 nt Rep3


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L5 site- L5 3MM 22 nt Rep3


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L5 site- L5 2MM 22 nt Rep3


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L5 site- L5 22MM 22 nt Rep3


title: SuperFi RNP electroporation at L18 site- L18 23 nt Rep2


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L5 site- L5 1MM 22 nt Rep3


title: SuperFi RNP electroporation at L5 site- L5 22 nt Rep5


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L5 site- L5 22MM 22 nt Rep2


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L5 site- L5 21MM 22 nt Rep2


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L5 site- L5 20MM 22 nt Rep2


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L5 site- L5 16MM 22 nt Rep3


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L5 site- L5 17MM 22 nt Rep2


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L5 site- L5 11MM 22 nt Rep3


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L5 site- L5 16MM 22 nt Rep2


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L9 site- L9 7MM 22 nt Rep1


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L5 site- L5 19MM 22 nt Rep2


title: SuperFi RNP electroporation at L5 site- L5 20 nt Rep2


title: SuperFi RNP electroporation at L18 site- L18 22 nt Rep2


title: SuperFi RNP Mismatched 22 nt gRNA electroporation at L9 site- L9 4MM 22 nt Rep1