title: aKO-KA

city: San Antonio

first: Chu

state: TX

address: 7703 Floyd Dr

department: Department of Cellular and Integrative Physiology

institute: The University of Texas Health Science Center at San Antonio

description:
Library name: ako_ka
10x Genomics

data_processing:
The demultiplexing, barcoded processing, gene counting generated by using Cell Ranger Count v9.0.1, sample integration performed in R.
Assembly: mm10
Supplementary files format and content: matrix h5 files

molecule: nuclear RNA

source_name: hippocampus

extract_protocol:
Tissue samples were subjected to lysis in 1 ml of lysis buffer (10 mM Tris-HCl, 5% Nonidet P40, 3 mM MgCl2, and 10 mM NaCl) along with 1 U/μl RNasin Plus RNase Inhibitor (Promega) for 15 minutes in a douncing tube placed on ice. The tissue was then homogenized using a loose pestle with ten dounces. The resulting suspension was filtered through a 70 μm mesh and centrifuged at 500 g for 5 minutes at 4°C. Post-lysis, the sample was treated with 2 ml of ice-cold nucleus wash buffer (5% wt/vol BSA, 0.25% wt/vol glycerol, 40 U/mL Protector RNAse inhibitor, 0.5X PBS), passed through a 20 μm mesh twice, and re-centrifuged under the same conditions. The supernatant was discarded, and the nuclear pellet was resuspended in 500 μl of wash buffer. An additional 500 μl of 50% (wt/vol) iodixanol (Optiprep) working solution was added to aid in further cleaning. This suspension was then layered over 1 mL of 29% (wt/vol) iodixanol solution in a 2 mL centrifuge tube and centrifuged at 10,000g for 30 minutes at 4°C. Finally, the nuclei were resuspended in a suitable volume of nuclei buffer to achieve a concentration of 500-1,000 nuclei/μl for subsequent capture.
Single nucleus RNA sequencing libraries were prepared using the Chromium Next GEM Single Cell 3′ Gel Bead v4 Kit (10x Genomics)

value: hippocampus

value: aKO

value: KA


title: ID8, KO-1

status: Public on Feb 18 2026

type: SRA

city: Hangzhou

first: Lv

last: Fei

address: The First People's Hospital of Hangzhou, Zhejiang Province

country: China

institute: Zhejiang Chinese Medical University

description: Library name: sample4

library_source: other

data_processing:
Raw sequencing reads were trimmed using Cutadapt to remove adapter sequences, and only reads of 25–34 nt in length were retained for downstream analysis.
To remove contaminating rRNA and tRNA reads, the filtered reads were aligned to rRNA and tRNA reference sequences using Bowtie.
The remaining reads were aligned to the mouse reference genome (GRCm38/mm10) and transcriptome using HISAT2 and Bowtie, respectively.
Ribosome profiling features, including trinucleotide periodicity and codon usage frequency, were calculated using riboWaltz, and actively translated open reading frames (ORFs) were identified with Ribotricer.
Read counts were quantified with featureCounts and normalized using DESeq2, followed by differential translation analysis and translation efficiency calculation by integrating corresponding mRNA-seq data.
Assembly: mm10
Supplementary files format and content: Tab-delimited text file, counts
Library strategy: Ribo-Seq

molecule: other

organism: Mus musculus

extract_protocol:
Cells were harvested after treatment with 100 µg/mL cycloheximide for 2 minutes to arrest ribosomes. Cell lysates were prepared in lysis buffer with cycloheximide and RNase inhibitors. Ribosome-protected fragments (RPFs, ~28-30 nt) were generated by digesting lysates with RNase I, followed by ribosome isolation via sucrose cushion centrifugation. RPFs were size-selected by gel purification, dephosphorylated, and ligated to a pre-adenylated linker for library construction.
Raw sequencing reads were trimmed of adapter sequences using Cutadapt. Reads with lengths between 25-34 nt were retained. rRNA and tRNA reads were removed by alignment to rRNA/tRNA sequences using Bowtie. Remaining reads were aligned to the reference genome (GRCm38/mm10 for mouse) and transcriptome using HISAT2 and Bowtie, respectively.

tag: cell line


title: WT-Veh

last: Chen

country: USA

description:
Library name: wt_veh
10x Genomics

library_source: transcriptomic single cell

tag: tissue

tag: genotype

value: WT

tag: treatment

value: Veh


title: aKO-Veh

description:
Library name: ako_veh
10x Genomics


title: LPD - female-rep-4

city: Nashville

first: Guoqiang

last: Gu

state: TN

address: 2213 Garland Avenue, MRB4, Rm 9415

department: Cell and Developmental Biology

institute: Vanderbilt University

description:
Library name: 8228-GG-24
beta-cell-LPD exposed

library_source: transcriptomic

data_processing:
Sequencing reads were trimmed to remove adapter sequences using Cutadapt (v2.10).
Trimmed reads were aligned to the Gencode GRCm38.p6 genome using STAR (v2.7.8a)
Gencode vM24 gene annotations were provided to STAR to improve the accuracy of mapping.
Homer was used to count the number of reads mapped to each gene.
For further anaylsis, DESeq2 and GSEA were used for biological funcitonal analyses.
Assembly: GRCm38.p6
Supplementary files format and content: Excel, raw counts of mapped reads. (Raw_counts_beta_cells_maternal_diet.xlsm)
Supplementary files format and content: Excel, normalized levels (FKPM_beta_cells_maternal_diet.xlsm)

molecule: total RNA

source_name: pancreatic islets

growth_protocol: Mice were housed in AAALAC-Accredited facilitty, following protocols approved by the Vanderbilt Unviersity IACUC for Guoqiang Gu.

extract_protocol:
Purified beta cells were dissolved in Trizol and used in a DNA-free RNA-isolation kit (Zymo R2050) for RNA purification.
Total RNA preps will subject to standard Sample Quality Control analysis by using fluorometry Qubit or Picogreen and integrity by BioAnalyzer or TapeStation.  Only those with a RIN>=8.1 were used for down-stream studies. For each sample, 50 ng of RNA were used for linrary preparation using the Illumina Ribo-Zero Plus rRNA Depletion kit. The procedure followed that specified by manufacturer, which include the depletion of rRNAs, cDNA reverse transcription, strand specific labeling/amplification. Library Quality Control analysis will be performed by using Qubit and BioAnalyzer to determine the concentration and size bp. Only those with significan DNA fragments between 150-1000 bp were sequenced. Each library that passes the final QC undergoes qPCR using the KAPA library quantification kit and QuantStudio. qPCR allows highly accurate quantification of library molecules that can seed clusters and balance target sample representation during pooling. Multiplexed samples are pooled in equimolar or user-defined ratios to achieve the multiplexing level requested by the user using Illumina MiSeq platform.

treatment_protocol: Islets were isolated from male and female mice. They were then dissocaited into single cells and FACSed for beta cells based in Apple expression. Purified beta cells were then used for RNA prep.

value: pancreatic islets

tag: cell type

value: Islet beta cells

value: LPD-exposed


title: LPD - female-rep-2

description:
Library name: 8228-GG-22
beta-cell-LPD exposed


title: LPD - female-rep-3

description:
Library name: 8228-GG-23
beta-cell-LPD exposed


title: ID8, WT-4

description:
Library name: sample1
Sample column name in All.count.txt: WT-1

data_processing:
Raw data (raw reads) of fastq format were firstly processed through fastp software.
Reference genome and gene model annotation files were downloaded from genome website directly. Index of the reference genome was built using Hisat2 v2.0.5 and paired-end clean reads were aligned to the reference genome using Hisat2 v2.0.5.
featureCounts v1.5.0-p3 was used to count the reads numbers mapped to each gene. And then FPKM of each gene was calculated based on the length of the gene and reads count mapped to this gene.
Assembly: mm10
Supplementary files format and content: tab-delimited text files include count values for each smple

extract_protocol:
Total RNA was extracted using TRIzol and the quality and quantity of total RNA were evaluated using an Agilent 2100 Bioanalyzer.
Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection.


title: ID8, WT-2

description: Library name: sample2


title: LPD - male-rep-4

description:
Library name: 8228-GG-20
beta-cell-LPD exposed


title: ID8, WT-3

description: Library name: sample3


title: WT-KA

description:
Library name: wt_ka
10x Genomics


title: LPD - male-rep-1

description:
Library name: 8228-GG-17
beta-cell-LPD exposed


title: ID8, KO-4

description:
Library name: sample4
Sample column name in All.count.txt: KO-1


title: LPD - male-rep-3

description:
Library name: 8228-GG-19
beta-cell-LPD exposed


title: HFD - male-rep-2

description:
Library name: 8228-GG-14
beta-cell-HFD exposed

value: HFD-wxposed


title: LPD - female-rep-1

description:
Library name: 8228-GG-21
beta-cell-LPD exposed


title: HFD - male-rep-3

description:
Library name: 8228-GG-15
beta-cell-HFD exposed


title: HFD - female-rep-3

description:
Library name: 8228-GG-11
beta-cell-HFD exposed


title: HFD - male-rep-4

description:
Library name: 8228-GG-16
beta-cell-HFD exposed


title: HFD - female-rep-2

description:
Library name: 8228-GG-10
beta-cell-HFD exposed


title: HFD - female-rep-1

description:
Library name: 8228-GG-9
beta-cell-HFD exposed


title: HFD - female-rep-4

description:
Library name: 8228-GG-12
beta-cell-HFD exposed


title: HFD - male-rep-1

description:
Library name: 8228-GG-13
beta-cell-HFD exposed


title: LPD - male-rep-2

description:
Library name: 8228-GG-18
beta-cell-LPD exposed


title: ND - male-rep-2

description:
Library name: 8228-GG-6
beta-cell  control

value: control


title: ND - male-rep-4

description:
Library name: 8228-GG-8
beta-cell  control


title: OB TNFa+BMP4_3

city: Okayama

first: Shigeki

last: Suzuki

state: Okayama

address: 2-5-1 Shikata-cho, Kita-ku

department: Department of Operative Dentistry

country: Japan

institute: Okayama University

description:
Library name: Sample 12
ss1633_8_TagDir/ (15511103.5 total)

data_processing:
Adapter trimming was conducted with Trim galore with default setting and then aligned to a reference genome (mm10) using HISAT2. The gene expression at exons was quantified using `analyzeRepeast.pl ` command in HOMER using `-strand both` and `-count exons` followed by `getDiffExpression.pl` in HOMER using -batch` to identify expressed genes.
Assembly: mm10
Supplementary files format and content: tab-delimited text files include FPKM values for all samples

molecule: polyA RNA

source_name: cell line

growth_protocol: MC3T3-E1 cells were maintained in α-MEM supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin.

extract_protocol:
RNA was isolated from MC3T3-E1 cells with RNAiso plus. Purified RNA was DNase-treated and further used for RNA-seq analyses.
Input RNA was converted to cDNA libraries using the NEBNExt poly(A) mRNA magnetic isolation module. Libraries were sequenced using 150-bp paired-end reads on an illumine Novaseq instruments.

treatment_protocol: MC3T3-E1 cells were serum-reduced in medium containing 2.5% FBS for 30 min, treated with or without TNF-α (10 ng/mL) for 30 min, and then stimulated with or without BMP4 (10 ng/mL) for 3 h.

value: cell line

value: mouse osteoblasts

value: TNFa (10ng/ml for 3 hrs)+BMP4 (10ng/ml for 3 hrs)

tag: batch


title: ND - female-rep-3

description:
Library name: 8228-GG-3
beta-cell  control


title: ND - female-rep-1

description:
Library name: 8228-GG-1
beta-cell  control


title: ND - male-rep-3

description:
Library name: 8228-GG-7
beta-cell  control


title: ND - male-rep-1

description:
Library name: 8228-GG-4
beta-cell  control


title: OB TNFa+BMP4_2

description:
Library name: Sample 11
ss1633_7_TagDir/ (20579256.5 total)


title: OB BMP4_3

description:
Library name: Sample 10
ss1633_6_TagDir/ (14378725.5 total)

value: BMP4 (10ng/ml for 3 hrs)


title: OB BMP4_2

description:
Library name: Sample 9
ss1633_5_TagDir/ (13737313.5 total)


title: ND - female-rep-4

description:
Library name: 8228-GG-5
beta-cell  control


title: OB none_2

description:
Library name: Sample 5
ss1633_1_TagDir/ (13890822.5 total)


title: OB TNFa_p65_2

description:
Library name: Sample 7
ss1633_3_TagDir/ (15292652.5 total)

value: TNFa

value: TNFa (10ng/ml for 3 hrs)


title: OB TNFa_p65_3

description:
Library name: Sample 8
ss1633_4_TagDir/ (14798763.5 total)


title: OB none_3

description:
Library name: Sample 6
ss1633_2_TagDir/ (15211103.0 total)


title: ND - female-rep-2

description:
Library name: 8228-GG-2
beta-cell  control


title: OB TNFa+BMP4_1

description:
Library name: Sample 4
ss1449_4_TagDir/ (13896102.5 total)


title: OB none_1

description:
Library name: Sample 1
ss1449_1_TagDir/ (14701538.5 total)


title: OB BMP4_1

description:
Library name: Sample 3
ss1449_3_TagDir/ (13889455.0 total)


title: OB TNFa_p65_1

description:
Library name: Sample 2
ss1449_2_TagDir/ (13804028.0 total)


title: OB_TNFa+BMP4_Smad4_2

description:
Library name: Sample 8
ss1448_D2_TagDir/ Tag Count in given bp (8430566.5 Total, normalization factor = 1, effective total = 10000000)

library_source: genomic

data_processing:
Reads were trimmed using Trim Galore v0.6.6 (with Cutadapt v3.3), quality-checked with FastQC, v0.11.9 and aligned to mm10 using Bowtie2 v2.5.1. PCR duplicates were removed using MarkDuplicates in Picard v2.25.1. Concordance between biological replicates was assessed from sorted BAM files using deepTools v3.5.1 multiBamSummary and Pearson correlation. Tag directories were generated using HOMER v4.11 makeTagDirectory, and peaks were called using HOMER getDifferentialPeaksReplicates.pl with -style factor to generate high-confidence peaks across replicates; peaks passing ≥2-fold enrichment and FDR < 0.05 relative to input were retained. The input chromatin DNA was utilized as a control.
Assembly: mm10
Supplementary files format and content: Tag Count in given bp (normalization factor = 1, effective total = 10000000)

molecule: genomic DNA

extract_protocol:
Cells were crosslinked with formaldehyde and ChIP was performed using the SimpleChIP® Plus Enzymatic Chromatin IP Kit with magnetic beads (Cell Signaling Technology) according to the manufacturer’s protocol. Equal amounts of chromatin (equivalent to chromatin from ~4 × 10⁶ cells) were immunoprecipitated using ChIP-validated anti-Smad4 (D3R4N) and anti-p65 (D14E12) antibodies. After reverse crosslinking and proteinase K treatment, DNA was purified using ChIP DNA Clean & Concentrator (Zymo Research, Irvine, CA, USA).
Library preparation, sequencing, and initial processing were performed by Novogene. Libraries were sequenced as 150-bp paired-end reads on a NovaSeq platform to a depth of >10 million mapped reads per sample.

tag: chip antibody


title: background_2

description:
Library name: Sample 10
ss1491_2_TagDir/ Tag Count in given bp (55862124.0 Total, normalization factor = 1, effective total = 10000000)

data_processing:
Reads were trimmed using Trimmomatic v0.39, aligned to mm10 using Bowtie2 v2.5.1, PCR duplicates were removed using MarkDuplicates in Picard v2.25.1, and replicate concordance was assessed using deepTools v3.5.1. Peaks were called using HOMER v4.11 using ultrasonicated DNA as a control. Differentially enriched peaks were identified across untreated, TNF-α–only, BMP4-only, and TNF-α+BMP4 conditions using HOMER mergePeaks with -d given and default settings.
Assembly: mm10
Supplementary files format and content: Tag Count in given bp (normalization factor = 1, effective total = 10000000)

extract_protocol:
Cells were processed using the ATAC-seq Kit (#53150; Active Motif, Carlsbad, CA, USA) according to the manufacturer’s protocol.
Libraries were sequenced as 150-bp paired-end reads on a NovaSeq platform.

value: sonicated DNA

value: sonicated DNA of MC3T3-E1 cells


title: OB_none_2

description:
Library name: Sample 2
ss1495_2_TagDir/ Tag Count in given bp (29118517.5 Total, normalization factor = 1, effective total = 10000000)


title: OB_TNFa_p65 2

description:
Library name: Sample 4
ss1495_4_TagDir/ Tag Count in given bp (19735770.5 Total, normalization factor = 1, effective total = 10000000)


title: OB_TNFa+BMP4_2

description:
Library name: Sample 8
ss1495_8_TagDir/ Tag Count in given bp (18842390.5 Total, normalization factor = 1, effective total = 10000000)


title: OB_BMP4_2

description:
Library name: Sample 6
ss1495_6_TagDir/ Tag Count in given bp (27922813.0 Total, normalization factor = 1, effective total = 10000000)


title: background_1

description:
Library name: Sample 9
ss1491_1_TagDir/ Tag Count in given bp (49527821.5 Total, normalization factor = 1, effective total = 10000000)


title: OB_input_1

description:
Library name: Sample 9
ss1448_In1_TagDir/ Tag Count in given bp (7612757.0 Total, normalization factor = 1, effective total = 10000000)
ss1448_In1_TagDir/ Tag Count in given bp (7612757.0 Total, normalization factor = 1, effective total = 10000000)
ss1448_In1_TagDir/ Tag Count in given bp (7612757.0 Total, normalization factor = 1, effective total = 10000000)
ss1448_In1_TagDir/ Tag Count in given bp (7612757.0 Total, normalization factor = 1, effective total = 10000000)

value: input DNA

value: Genomic DNA was pooled in equal amounts from MC3T3-E1 cells stimulated with TNFa, BMP4, or TNFa+BMP4.

value: input


title: OB_BMP4_1

description:
Library name: Sample 5
ss1495_5_TagDir/ Tag Count in given bp (26048595.0 Total, normalization factor = 1, effective total = 10000000)


title: OB_TNFa+BMP4_Smad4_1

description:
Library name: Sample 7
ss1448_D1_TagDir/ Tag Count in given bp (8641200.0 Total, normalization factor = 1, effective total = 10000000)


title: OB_none_OC1_2

description: Library name: Sample 2

data_processing:
The informative single-end read carrying the viewpoint primer was processed using pipe4C v1.1  with default workflows for adapter and restriction-site trimming, alignment to mm10, fragment mapping, and QC reporting.
Assembly: mm10
Supplementary files format and content: bigwig
Supplementary files format and content: FASTQ
Supplementary files format and content: rds
Supplementary files format and content: bedgraph
Library strategy: 4C-Seq

extract_protocol:
Cells were crosslinked with 2% formaldehyde for 10 min and quenched with 125 mM glycine. Cells were washed with cold D-PBS, harvested, and pelleted (1,000 × g, 5 min). Nuclei were isolated using lysis buffer (140 mM NaCl, 1 mM EDTA, 0.5% IGEPAL CA-630, 1% Triton X-100, 50 mM HEPES-KOH pH 7.5) with protease inhibitors (cOmplete, EDTA-free; Sigma-Aldrich, St. Louis, MO, USA).
Libraries were sequenced as 150-bp paired-end reads on a NovaSeq platform.

tag: view point


title: OB_TNFa_OC2_1

description: Library name: Sample 9


title: OB_none_OC1_1

description: Library name: Sample 1


title: OB_TNFa+BMP4_OC2_1

description: Library name: Sample 11


title: OB_TNFa_OC1_1

description: Library name: Sample 3


title: OB_TNFa_p65 1

description:
Library name: Sample 3
ss1495_3_TagDir/ Tag Count in given bp (16486612.5 Total, normalization factor = 1, effective total = 10000000)


title: OB_TNFa+BMP4_OC1_2

description: Library name: Sample 6


title: OB_TNFa_OC1_2

description: Library name: Sample 4


title: OB_TNFa_OC2_2

description: Library name: Sample 10


title: OB_TNFa+BMP4_OC1_1

description: Library name: Sample 5


title: OB_none_OC2_2

description: Library name: Sample 8


title: OB_TNFa+BMP4_OC2_2

description: Library name: Sample 12


title: OB_none_OC2_1

description: Library name: Sample 7


title: OB_TNFa+BMP4_p65_2

description:
Library name: Sample 6
ss1448_C2_TagDir/ Tag Count in given bp (7471422.0 Total, normalization factor = 1, effective total = 10000000)


title: OB_none_1

description:
Library name: Sample 1
ss1495_1_TagDir/ Tag Count in given bp (30051723.0 Total, normalization factor = 1, effective total = 10000000)


title: OB_TNFa+BMP4_1

description:
Library name: Sample 7
ss1495_7_TagDir/ Tag Count in given bp (21757662.0 Total, normalization factor = 1, effective total = 10000000)


title: OB_input_2

description:
Library name: Sample 10
ss1448_In2_TagDir/ Tag Count in given bp (8265598.5 Total, normalization factor = 1, effective total = 10000000)
ss1448_In2_TagDir/ Tag Count in given bp (8265598.5 Total, normalization factor = 1, effective total = 10000000)
ss1448_In2_TagDir/ Tag Count in given bp (8265598.5 Total, normalization factor = 1, effective total = 10000000)
ss1448_In2_TagDir/ Tag Count in given bp (8265598.5 Total, normalization factor = 1, effective total = 10000000)


title: OB_TNFa+BMP4_p65_1

description:
Library name: Sample 5
ss1448_C1_TagDir/ Tag Count in given bp (9201011.5 Total, normalization factor = 1, effective total = 10000000)


title: OB_BMP4_Smad4_1

description:
Library name: Sample 3
ss1448_B1_TagDir/ Tag Count in given bp (9439017.0 Total, normalization factor = 1, effective total = 10000000)


title: OB_TNFa_p65_2

description:
Library name: Sample 2
ss1448_A2_TagDir/ Tag Count in given bp (9089124.0 Total, normalization factor = 1, effective total = 10000000)


title: Control islet cells-Rep-1

first: guoqiang

state: Tennessee

address: 9415D Medical Research Building IV

department: Department of Cell and Developmental Biology

institute: Vanderbilt University Medical Center

description:
Library name: 11631-GG-2
PIPseq3

data_processing:
DropEst was used to generate count matrices.
Cells with low unique mapping reads (<500), low proportion of expressed genes (<100), or high proportion of mitochondrial RNAs (>10%) will be excluded.
Reads were normalized using UMI-filtered counts.
Cell subpopulations were identified and visualized by UMAP using Seurat based on the first 30 principal components generated from the top 2000 highly variable genes.
Assembly: GRCm39
Supplementary files format and content: tab-separated values files and matrix files

source_name: pancreas islet

growth_protocol: Mouse islets were isoalted from adult mice fed with normal diet.

extract_protocol:
Live cells were directly used in PIPseq. Libraries were made following instructions provided by manufacturer. No RNA exaction step was needed.
The end library was sequenced with Novaseq 6000 (Illumina), targeting 120 million reads per sample.

treatment_protocol: Isolated islets were immediately dissociated for PIPSeq3

value: pancreas islet

value: controls


title: WholeCells_20plex-multiscQers_SYFP2pos_STR_GEX_L001

city: Seattle

first: John

middle: Kenneth

last: Mich

state: WA

address: 615 Westlake Ave N

institute: Allen Institute for Brain Science

description:
Library name: Load2234C_GEX-2234C_L001
YFP-expressing whole cells sorted from C57Bl/6 cell suspensions using FACS. These sorted cells were then prepared for scRNA-seq using the 10X v3.1 3' GEX droplet-based sc/snRNA-seq platform. cDNA and sequencing libraries were prepared according to the manufacturer's protocols, and then sequenced to a depth of approx 125k read pairs per cell. Additionally, cDNA libraries were amplified with custom PCR primers to maximize detection of AAV-encoded mBC barcodes, and supernatant from cDNA purification was amplified with different custom PCR primers to detect AAV-encoded oBC barcodes.  GEX and mBC barcode and oBC barcode libraries were each sequenced.

data_processing:
Raw fastq files are provided from Illumina NovaSeq 6000 on-board base-calling protocol.
For the CellXGene processed data matrices, we aligned reads to mm10 and called cells using CellRanger v6.1.1 using default parameters and built-in STAR v2.7.2a.
We performed the following filtering steps to include only high-quality cells [or nuclei]:  1) >2000 genes detected [>1000 for nuclei].  2) <20% of reads mitochondrial [<5% for nuclei].  3) Doublet score < 0.3 using DoubletFinder, https://github.com/chris-mcginnis-ucsf/DoubletFinder. These high-quality cells [or nuclei] are provided.
For the mBC and oBC libraries, we specifically amplified from cDNA using nested PCR conditions using custom indexed primers.  In the case of the oBC libraries we amplified from low-molecular weight cDNA supernatant.  See manuscript for primer sequences and conditions, https://www.biorxiv.org/content/10.1101/2025.01.14.633018v1. These were sequenced alongside cDNA libraries.
To enumerate barcode UMIs and reads, we processed fastq files using a custom python script.  See manuscript for code, https://www.biorxiv.org/content/10.1101/2025.01.14.633018v1.
Assembly: mm10

source_name: striatal whole single cells

extract_protocol:
10X Genomics Chromium NextGEM Single Cell v3.1 3' GEX kit
Followed manufacturer's protocol (CG000204 Revision D)

value: striatal whole single cells

value: sorted YFP-expressing whole single cell

value: 20plex-multiscQers PHP.eB


title: WT-rep1

city: Cambridge

first: Mohamed

middle: A.

last: El-Brolosy

state: MA

address: 455 Main Street

institute: Whitehead Institute for Biomedical Research

description: Library name: Sample1

data_processing:
Reads were processed and aligned to the Ensembl mouse genome version mm10 (GRCm38) as described before (Vlaming et al., NSMB, 2022 & Stein et al., Mol Cell, 2022).
Assembly: mm10
Supplementary files format and content: Spike (1st group) and Depth (2nd group) normalized bedgraphs

extract_protocol:
Cells were lysed in TRIzol and 4sU-labeled RNA was isolated, followed by library construction as described before (Vlaming et al., NSMB, 2022 & Stein et al., Mol Cell, 2022).
Library construction as described before (Vlaming et al., NSMB, 2022 & Stein et al., Mol Cell, 2022).

value: Mouse embryonic fibroblasts

value: Fibroblast

value: WT


title: Actg1-NSD;∆Ilf3 -bach3-rep1

description: Library name: Sample3

data_processing:
Reads were processed and aligned to the Ensembl mouse genome version mm10 (GRCm38) as described before (Mahat et al., 2016; Mimoso and Adelman, 2023).
Assembly: mm10
Supplementary files format and content: tab-delimited file with normalized readcounts for each sample in each batch, DeSeq2 analysis file, and spike and normalized bedGraphs.
Library strategy: PRO-Seq

extract_protocol:
2 million cells that were permeabilized, followed by library construction as described before (Mahat et al., 2016; Mimoso and Adelman, 2023).
2 million cells  were permeabilized, followed by library construction as described before (Mahat et al., 2016; Mimoso and Adelman, 2023).

value: cell line

value: WT;ILF3 KO


title: WT-batch3-rep1

description: Library name: Sample1


title: WholeCells_20plex-multiscQers_SYFP2pos_STR_mBC

description:
Library name: Load2234C_mBC
YFP-expressing whole cells sorted from C57Bl/6 cell suspensions using FACS. These sorted cells were then prepared for scRNA-seq using the 10X v3.1 3' GEX droplet-based sc/snRNA-seq platform. cDNA and sequencing libraries were prepared according to the manufacturer's protocols, and then sequenced to a depth of approx 125k read pairs per cell. Additionally, cDNA libraries were amplified with custom PCR primers to maximize detection of AAV-encoded mBC barcodes, and supernatant from cDNA purification was amplified with different custom PCR primers to detect AAV-encoded oBC barcodes.  GEX and mBC barcode and oBC barcode libraries were each sequenced.


title: Actg1-NSD;∆Ilf3-rep1

description: Library name: Sample5

value: Actg1-NSD;ILF3 KO


title: Actg1-NSD-rep1

description: Library name: Sample3


title: WholeCells_20plex-multiscQers_SYFP2pos_STR_oBC

description:
Library name: Load2234C_oBC
YFP-expressing whole cells sorted from C57Bl/6 cell suspensions using FACS. These sorted cells were then prepared for scRNA-seq using the 10X v3.1 3' GEX droplet-based sc/snRNA-seq platform. cDNA and sequencing libraries were prepared according to the manufacturer's protocols, and then sequenced to a depth of approx 125k read pairs per cell. Additionally, cDNA libraries were amplified with custom PCR primers to maximize detection of AAV-encoded mBC barcodes, and supernatant from cDNA purification was amplified with different custom PCR primers to detect AAV-encoded oBC barcodes.  GEX and mBC barcode and oBC barcode libraries were each sequenced.


title: WholeCells_20plex-multiscQers_SYFP2pos_STR_GEX_L002

description:
Library name: Load2234C_GEX-2234C_L002
YFP-expressing whole cells sorted from C57Bl/6 cell suspensions using FACS. These sorted cells were then prepared for scRNA-seq using the 10X v3.1 3' GEX droplet-based sc/snRNA-seq platform. cDNA and sequencing libraries were prepared according to the manufacturer's protocols, and then sequenced to a depth of approx 125k read pairs per cell. Additionally, cDNA libraries were amplified with custom PCR primers to maximize detection of AAV-encoded mBC barcodes, and supernatant from cDNA purification was amplified with different custom PCR primers to detect AAV-encoded oBC barcodes.  GEX and mBC barcode and oBC barcode libraries were each sequenced.


title: Actg1-NSD-rep2

description: Library name: Sample4


title: Actg1-NSD;∆Ilf3-rep2

description: Library name: Sample6


title: WT-rep2

description: Library name: Sample2


title: Actg1-NSD;∆Ilf3 -bach3-rep2

description: Library name: Sample4


title: WT-batch3-rep2

description: Library name: Sample2


title: Control islet cells-Rep-2

description:
Library name: 11631-GG-4
PIPseq3


title: WholeCells_20plex-multiscQers_SYFP2pos_ALM_GEX_L001

description:
Library name: Load2234A_GEX-2234A_L001
YFP-expressing whole cells sorted from C57Bl/6 cell suspensions using FACS. These sorted cells were then prepared for scRNA-seq using the 10X v3.1 3' GEX droplet-based sc/snRNA-seq platform. cDNA and sequencing libraries were prepared according to the manufacturer's protocols, and then sequenced to a depth of approx 125k read pairs per cell. Additionally, cDNA libraries were amplified with custom PCR primers to maximize detection of AAV-encoded mBC barcodes, and supernatant from cDNA purification was amplified with different custom PCR primers to detect AAV-encoded oBC barcodes.  GEX and mBC barcode and oBC barcode libraries were each sequenced.

source_name: anterior lateral motor cortex whole single cells


title: Mutant islet cells-Rep-1

description:
Library name: 11631-GG-1
PIPseq3

value: Myt3 mutant


title: WT;∆Ilf3-rep2

description: Library name: Sample8


title: OB_BMP4_Smad4_2

description:
Library name: Sample 4
ss1448_B2_TagDir/ Tag Count in given bp (9156012.0 Total, normalization factor = 1, effective total = 10000000)

value: mouse osteoblasts

value: BMP4 (10ng/ml for 3 hrs)


title: OB_TNFa_p65_1

description:
Library name: Sample 1
ss1448_A1_TagDir/ Tag Count in given bp (7470155.0 Total, normalization factor = 1, effective total = 10000000)

value: TNFa

value: TNFa (10ng/ml for 3 hrs)


title: Mutant islet cells-Rep-2

description:
Library name: 11631-GG-3
PIPseq3


title: WholeCells_20plex-multiscQers_SYFP2pos_ALM_mBC

description:
Library name: Load2234A_mBC
YFP-expressing whole cells sorted from C57Bl/6 cell suspensions using FACS. These sorted cells were then prepared for scRNA-seq using the 10X v3.1 3' GEX droplet-based sc/snRNA-seq platform. cDNA and sequencing libraries were prepared according to the manufacturer's protocols, and then sequenced to a depth of approx 125k read pairs per cell. Additionally, cDNA libraries were amplified with custom PCR primers to maximize detection of AAV-encoded mBC barcodes, and supernatant from cDNA purification was amplified with different custom PCR primers to detect AAV-encoded oBC barcodes.  GEX and mBC barcode and oBC barcode libraries were each sequenced.

value: anterior lateral motor cortex whole single cells


title: WholeCells_20plex-multiscQers_SYFP2pos_ALM_oBC

description:
Library name: Load2234A_oBC
YFP-expressing whole cells sorted from C57Bl/6 cell suspensions using FACS. These sorted cells were then prepared for scRNA-seq using the 10X v3.1 3' GEX droplet-based sc/snRNA-seq platform. cDNA and sequencing libraries were prepared according to the manufacturer's protocols, and then sequenced to a depth of approx 125k read pairs per cell. Additionally, cDNA libraries were amplified with custom PCR primers to maximize detection of AAV-encoded mBC barcodes, and supernatant from cDNA purification was amplified with different custom PCR primers to detect AAV-encoded oBC barcodes.  GEX and mBC barcode and oBC barcode libraries were each sequenced.


title: WholeCells_20plex-multiscQers_SYFP2pos_ALM_GEX_L002

description:
Library name: Load2234A_GEX-2234A_L002
YFP-expressing whole cells sorted from C57Bl/6 cell suspensions using FACS. These sorted cells were then prepared for scRNA-seq using the 10X v3.1 3' GEX droplet-based sc/snRNA-seq platform. cDNA and sequencing libraries were prepared according to the manufacturer's protocols, and then sequenced to a depth of approx 125k read pairs per cell. Additionally, cDNA libraries were amplified with custom PCR primers to maximize detection of AAV-encoded mBC barcodes, and supernatant from cDNA purification was amplified with different custom PCR primers to detect AAV-encoded oBC barcodes.  GEX and mBC barcode and oBC barcode libraries were each sequenced.


title: WT;∆Ilf3-rep1

description: Library name: Sample7


title: WholeCells_20plex-singlescQers_SYFP2pos_STR_GEX

description:
Library name: Load3066C_NW-TX34041-12
YFP-expressing whole cells sorted from C57Bl/6 cell suspensions using FACS. These sorted cells were then prepared for scRNA-seq using the 10X v3.1 3' GEX droplet-based sc/snRNA-seq platform. cDNA and sequencing libraries were prepared according to the manufacturer's protocols, and then sequenced to a depth of approx 125k read pairs per cell. Additionally, cDNA libraries were amplified with custom PCR primers to maximize detection of AAV-encoded mBC barcodes, and supernatant from cDNA purification was amplified with different custom PCR primers to detect AAV-encoded oBC barcodes.  GEX and mBC barcode and oBC barcode libraries were each sequenced.

value: 20plex-singlescQers PHP.eB


title: WholeCells_20plex-singlescQers_SYFP2neg_ALM_GEX

description:
Library name: Load3066B_NW-TX34041-11
YFP-non-expressing whole cells sorted from C57Bl/6 cell suspensions using FACS. These sorted cells were then prepared for scRNA-seq using the 10X v3.1 3' GEX droplet-based sc/snRNA-seq platform. cDNA and sequencing libraries were prepared according to the manufacturer's protocols, and then sequenced to a depth of approx 125k read pairs per cell. Additionally, cDNA libraries were amplified with custom PCR primers to maximize detection of AAV-encoded mBC barcodes, and supernatant from cDNA purification was amplified with different custom PCR primers to detect AAV-encoded oBC barcodes.  GEX and mBC barcode and oBC barcode libraries were each sequenced.

value: sorted YFP-non-expressing whole single cell


title: WholeCells_20plex-singlescQers_SYFP2pos_ALM_oBC

description:
Library name: Load3066A_oBC
YFP-expressing whole cells sorted from C57Bl/6 cell suspensions using FACS. These sorted cells were then prepared for scRNA-seq using the 10X v3.1 3' GEX droplet-based sc/snRNA-seq platform. cDNA and sequencing libraries were prepared according to the manufacturer's protocols, and then sequenced to a depth of approx 125k read pairs per cell. Additionally, cDNA libraries were amplified with custom PCR primers to maximize detection of AAV-encoded mBC barcodes, and supernatant from cDNA purification was amplified with different custom PCR primers to detect AAV-encoded oBC barcodes.  GEX and mBC barcode and oBC barcode libraries were each sequenced.


title: WholeCells_20plex-singlescQers_SYFP2pos_STR_oBC

description:
Library name: Load3066C_oBC
YFP-expressing whole cells sorted from C57Bl/6 cell suspensions using FACS. These sorted cells were then prepared for scRNA-seq using the 10X v3.1 3' GEX droplet-based sc/snRNA-seq platform. cDNA and sequencing libraries were prepared according to the manufacturer's protocols, and then sequenced to a depth of approx 125k read pairs per cell. Additionally, cDNA libraries were amplified with custom PCR primers to maximize detection of AAV-encoded mBC barcodes, and supernatant from cDNA purification was amplified with different custom PCR primers to detect AAV-encoded oBC barcodes.  GEX and mBC barcode and oBC barcode libraries were each sequenced.

value: striatal whole single cells


title: WholeCells_20plex-singlescQers_SYFP2pos_ALM_GEX

description:
Library name: Load3066A_NW-TX34041-10
YFP-expressing whole cells sorted from C57Bl/6 cell suspensions using FACS. These sorted cells were then prepared for scRNA-seq using the 10X v3.1 3' GEX droplet-based sc/snRNA-seq platform. cDNA and sequencing libraries were prepared according to the manufacturer's protocols, and then sequenced to a depth of approx 125k read pairs per cell. Additionally, cDNA libraries were amplified with custom PCR primers to maximize detection of AAV-encoded mBC barcodes, and supernatant from cDNA purification was amplified with different custom PCR primers to detect AAV-encoded oBC barcodes.  GEX and mBC barcode and oBC barcode libraries were each sequenced.

value: sorted YFP-expressing whole single cell


title: Nuclei_AiP13244_SYFP2pos_CTX_GEX_L002

description:
Library name: LoadCN3244_L002
YFP-expressing nuclei sorted from nuclei suspensions using FACS. These sorted nuclei were prepared for scRNA-seq using the 10X v3.1 3' GEX droplet-based sc/snRNA-seq platform. cDNA and sequencing libraries were prepared according to the manufacturer's protocols, and then sequenced to a depth of approx 125k read pairs per cell.

source_name: cortical single nuclei

value: cortical single nuclei

value: sorted YFP-expressing single nucleus

value: AiP13244 PHP.eB


title: Nuclei_AiP13257_SYFP2pos_Fresh_CTX_GEX

description:
Library name: Load0962C_NW-TX0196-6
YFP-expressing nuclei sorted from nuclei suspensions using FACS. These sorted nuclei were freshly prepared for scRNA-seq using the 10X v3.1 3' GEX droplet-based sc/snRNA-seq platform. cDNA and sequencing libraries were prepared according to the manufacturer's protocols, and then sequenced to a depth of approx 125k read pairs per cell.

value: AiP13257 PHP.eB


title: Nuclei_AiP13247_SYFP2pos_CTX_GEX_L002

description:
Library name: LoadCN3247_L002
YFP-expressing nuclei sorted from nuclei suspensions using FACS. These sorted nuclei were prepared for scRNA-seq using the 10X v3.1 3' GEX droplet-based sc/snRNA-seq platform. cDNA and sequencing libraries were prepared according to the manufacturer's protocols, and then sequenced to a depth of approx 125k read pairs per cell.

value: AiP13247 PHP.eB


title: WholeCells_20plexIndividual_SCID_SYFP2neg_STR_GEX

description:
Library name: Load2214D_NW-TX4111-5
YFP-expressing whole cells sorted from SCID cell suspensions using FACS. These sorted cells were then prepared for scRNA-seq using the 10X v3.1 3' GEX droplet-based sc/snRNA-seq platform. cDNA and sequencing libraries were prepared according to the manufacturer's protocols, and then sequenced to a depth of approx 125k read pairs per cell. Additionally, cDNA libraries were amplified with custom PCR primers to maximize detection of AAV-encoded barcodes.  GEX and AAV Barcode libraries were each sequenced.

value: SCID

value: 20plexIndividual PHP.eB


title: WholeCells_20plexIndividual_SCID_SYFP2pos_STR_GEX

description:
Library name: Load2214C_NW-TX4111-3
YFP-expressing whole cells sorted from SCID cell suspensions using FACS. These sorted cells were then prepared for scRNA-seq using the 10X v3.1 3' GEX droplet-based sc/snRNA-seq platform. cDNA and sequencing libraries were prepared according to the manufacturer's protocols, and then sequenced to a depth of approx 125k read pairs per cell. Additionally, cDNA libraries were amplified with custom PCR primers to maximize detection of AAV-encoded barcodes.  GEX and AAV Barcode libraries were each sequenced.


title: WholeCells_20plexIndividual_SCID_SYFP2neg_ALM_GEX

description:
Library name: Load2214B_NW-TX4110-2
YFP-expressing whole cells sorted from SCID cell suspensions using FACS. These sorted cells were then prepared for scRNA-seq using the 10X v3.1 3' GEX droplet-based sc/snRNA-seq platform. cDNA and sequencing libraries were prepared according to the manufacturer's protocols, and then sequenced to a depth of approx 125k read pairs per cell. Additionally, cDNA libraries were amplified with custom PCR primers to maximize detection of AAV-encoded barcodes.  GEX and AAV Barcode libraries were each sequenced.


title: WholeCells_6plexPooled_SYFP2pos_STR_mBC

description:
Library name: Load1420C_NW-TX4022-3_mBC
YFP-expressing whole cells sorted from cell suspensions using FACS. These sorted cells were then prepared for scRNA-seq using the 10X v3.1 3' GEX droplet-based sc/snRNA-seq platform. cDNA and sequencing libraries were prepared according to the manufacturer's protocols, and then sequenced to a depth of approx 125k read pairs per cell. Additionally, cDNA libraries were amplified with custom PCR primers to maximize detection of AAV-encoded barcodes.  GEX and AAV Barcode libraries were each sequenced.

value: 6plexPooled PHP.eB


title: WholeCells_20plexIndividual_C57Bl/6J_SYFP2pos_ALM_GEX

description:
Library name: Load1305A_SQ_TX4038-6
YFP-expressing whole cells sorted from C57Bl/6 cell suspensions using FACS. These sorted cells were then prepared for scRNA-seq using the 10X v3.1 3' GEX droplet-based sc/snRNA-seq platform. cDNA and sequencing libraries were prepared according to the manufacturer's protocols, and then sequenced to a depth of approx 125k read pairs per cell. Additionally, cDNA libraries were amplified with custom PCR primers to maximize detection of AAV-encoded barcodes.  GEX and AAV Barcode libraries were each sequenced.


title: WholeCells_20plexIndividual_SCID_SYFP2pos_ALM_GEX

description:
Library name: Load2214A_NW-TX4109-2
YFP-expressing whole cells sorted from SCID cell suspensions using FACS. These sorted cells were then prepared for scRNA-seq using the 10X v3.1 3' GEX droplet-based sc/snRNA-seq platform. cDNA and sequencing libraries were prepared according to the manufacturer's protocols, and then sequenced to a depth of approx 125k read pairs per cell. Additionally, cDNA libraries were amplified with custom PCR primers to maximize detection of AAV-encoded barcodes.  GEX and AAV Barcode libraries were each sequenced.


title: WholeCells_20plexIndividual_SCID_SYFP2pos_STR_mBC

description:
Library name: Load2214C_NW-TX4111-3_mBC
YFP-expressing whole cells sorted from SCID cell suspensions using FACS. These sorted cells were then prepared for scRNA-seq using the 10X v3.1 3' GEX droplet-based sc/snRNA-seq platform. cDNA and sequencing libraries were prepared according to the manufacturer's protocols, and then sequenced to a depth of approx 125k read pairs per cell. Additionally, cDNA libraries were amplified with custom PCR primers to maximize detection of AAV-encoded barcodes.  GEX and AAV Barcode libraries were each sequenced.


title: WholeCells_20plexIndividual_SCID_SYFP2pos_ALM_mBC

description:
Library name: Load2214A_NW-TX4109-2_mBC
YFP-expressing whole cells sorted from SCID cell suspensions using FACS. These sorted cells were then prepared for scRNA-seq using the 10X v3.1 3' GEX droplet-based sc/snRNA-seq platform. cDNA and sequencing libraries were prepared according to the manufacturer's protocols, and then sequenced to a depth of approx 125k read pairs per cell. Additionally, cDNA libraries were amplified with custom PCR primers to maximize detection of AAV-encoded barcodes.  GEX and AAV Barcode libraries were each sequenced.


title: Nuclei_AiP13244_SYFP2pos_CTX_GEX_L001

description:
Library name: LoadCN3244_L001
YFP-expressing nuclei sorted from nuclei suspensions using FACS. These sorted nuclei were prepared for scRNA-seq using the 10X v3.1 3' GEX droplet-based sc/snRNA-seq platform. cDNA and sequencing libraries were prepared according to the manufacturer's protocols, and then sequenced to a depth of approx 125k read pairs per cell.


title: WholeCells_20plexIndividual_C57Bl/6J_SYFP2pos_ALM_mBC

description:
Library name: Load1305A_SQ_TX4038-6_mBC
YFP-expressing whole cells sorted from C57Bl/6 cell suspensions using FACS. These sorted cells were then prepared for scRNA-seq using the 10X v3.1 3' GEX droplet-based sc/snRNA-seq platform. cDNA and sequencing libraries were prepared according to the manufacturer's protocols, and then sequenced to a depth of approx 125k read pairs per cell. Additionally, cDNA libraries were amplified with custom PCR primers to maximize detection of AAV-encoded barcodes.  GEX and AAV Barcode libraries were each sequenced.


title: WholeCells_6plexPooled_SYFP2pos_ALM_mBC

description:
Library name: Load1420A_NW-TX4022-1_mBC
YFP-expressing whole cells sorted from cell suspensions using FACS. These sorted cells were then prepared for scRNA-seq using the 10X v3.1 3' GEX droplet-based sc/snRNA-seq platform. cDNA and sequencing libraries were prepared according to the manufacturer's protocols, and then sequenced to a depth of approx 125k read pairs per cell. Additionally, cDNA libraries were amplified with custom PCR primers to maximize detection of AAV-encoded barcodes.  GEX and AAV Barcode libraries were each sequenced.


title: Nuclei_AiP13257_SYFP2pos_Frozen_CTX_GEX

description:
Library name: Load0962A_NW-TX0196-4
YFP-expressing nuclei sorted from nuclei suspensions using FACS. These sorted nuclei were first frozen, then prepared for scRNA-seq using the 10X v3.1 3' GEX droplet-based sc/snRNA-seq platform. cDNA and sequencing libraries were prepared according to the manufacturer's protocols, and then sequenced to a depth of approx 125k read pairs per cell.


title: WholeCells_20plexIndividual_C57Bl/6J_SYFP2pos_STR_mBC

description:
Library name: Load1305C_SQ_TX4038-8_mBC
YFP-expressing whole cells sorted from C57Bl/6 cell suspensions using FACS. These sorted cells were then prepared for scRNA-seq using the 10X v3.1 3' GEX droplet-based sc/snRNA-seq platform. cDNA and sequencing libraries were prepared according to the manufacturer's protocols, and then sequenced to a depth of approx 125k read pairs per cell. Additionally, cDNA libraries were amplified with custom PCR primers to maximize detection of AAV-encoded barcodes.  GEX and AAV Barcode libraries were each sequenced.


title: WholeCells_20plexIndividual_C57Bl/6J_SYFP2pos_STR_GEX

description:
Library name: Load1305C_SQ_TX4038-8
YFP-expressing whole cells sorted from C57Bl/6 cell suspensions using FACS. These sorted cells were then prepared for scRNA-seq using the 10X v3.1 3' GEX droplet-based sc/snRNA-seq platform. cDNA and sequencing libraries were prepared according to the manufacturer's protocols, and then sequenced to a depth of approx 125k read pairs per cell. Additionally, cDNA libraries were amplified with custom PCR primers to maximize detection of AAV-encoded barcodes.  GEX and AAV Barcode libraries were each sequenced.


title: WholeCells_6plexPooled_SYFP2pos_STR_GEX

description:
Library name: Load1420C_NW-TX4022-3
YFP-expressing whole cells sorted from cell suspensions using FACS. These sorted cells were then prepared for scRNA-seq using the 10X v3.1 3' GEX droplet-based sc/snRNA-seq platform. cDNA and sequencing libraries were prepared according to the manufacturer's protocols, and then sequenced to a depth of approx 125k read pairs per cell. Additionally, cDNA libraries were amplified with custom PCR primers to maximize detection of AAV-encoded barcodes.  GEX and AAV Barcode libraries were each sequenced.


title: Male, Old, Middle-start Rapamycin+Acarbose, rep 2

status: Public on Feb 23 2026

city: Boston

first: Alexander

last: Tyshkovskiy

state: Massachusetts

address: 77 Avenue Louis Pasteur

institute: Brigham & Women's Hospital, Harvard Medical School

description:
Library name: UM78519
Male_Old_RaAc16_2

data_processing:
Reads were mapped to mouse genome (GRCm39) with STAR (version 2.7.11b). Genes were counted via featureCounts (version 2.0.6).
Assembly: GRCm39
Supplementary files format and content: comma-separated values file includes gene counts for each sample

source_name: Liver

extract_protocol:
RNA was extracted with PureLink RNA Mini Kit.
Libraries were prepared with TruSeq Stranded mRNA LT Sample Prep Kit according to TruSeq Stranded mRNA Sample Preparation Guide.

treatment_protocol: Mice were housed at the University of Michigan Medical School (UM), the University of Texas (UT), and The Jackson Laboratory (TJL). Animals were fed ad libitum with the Purina 5LG6 diet, produced in the same commercial diet facility (TestDiet, Richmond, IN, USA). Compounds were administered through food until the animals were sacrificed.

value: Liver

tag: strain

tag: Sex

value: Male

tag: cohort

tag: site

value: UM

value: Middle-start Rapamycin+Acarbose

tag: treatment abbreviation

tag: dose

tag: age group

value: Old

tag: age

value: 22 months

tag: age at_start_of_treatment

value: 16 months

tag: replicate


title: Nuclei_AiP13247_SYFP2pos_CTX_GEX_L001

description:
Library name: LoadCN3247_L001
YFP-expressing nuclei sorted from nuclei suspensions using FACS. These sorted nuclei were prepared for scRNA-seq using the 10X v3.1 3' GEX droplet-based sc/snRNA-seq platform. cDNA and sequencing libraries were prepared according to the manufacturer's protocols, and then sequenced to a depth of approx 125k read pairs per cell.

tag: tissue


title: WholeCells_20plex-singlescQers_SYFP2pos_STR_mBC

description:
Library name: Load3066C_mBC
YFP-expressing whole cells sorted from C57Bl/6 cell suspensions using FACS. These sorted cells were then prepared for scRNA-seq using the 10X v3.1 3' GEX droplet-based sc/snRNA-seq platform. cDNA and sequencing libraries were prepared according to the manufacturer's protocols, and then sequenced to a depth of approx 125k read pairs per cell. Additionally, cDNA libraries were amplified with custom PCR primers to maximize detection of AAV-encoded mBC barcodes, and supernatant from cDNA purification was amplified with different custom PCR primers to detect AAV-encoded oBC barcodes.  GEX and mBC barcode and oBC barcode libraries were each sequenced.

value: striatal whole single cells

tag: genotype

value: 20plex-singlescQers PHP.eB


title: WholeCells_6plexIndividual_SYFP2pos_ALM_mBC

description:
Library name: Load1249A_NW-TX4009-2_mBC
YFP-expressing whole cells sorted from cell suspensions using FACS. These sorted cells were then prepared for scRNA-seq using the 10X v3.1 3' GEX droplet-based sc/snRNA-seq platform. cDNA and sequencing libraries were prepared according to the manufacturer's protocols, and then sequenced to a depth of approx 125k read pairs per cell. Additionally, cDNA libraries were amplified with custom PCR primers to maximize detection of AAV-encoded barcodes.  GEX and AAV Barcode libraries were each sequenced.

value: anterior lateral motor cortex whole single cells

value: sorted YFP-expressing whole single cell

tag: treatment

value: 6plexIndividual PHP.eB


title: WholeCells_6plexPooled_SYFP2pos_ALM_GEX

description:
Library name: Load1420A_NW-TX4022-1
YFP-expressing whole cells sorted from cell suspensions using FACS. These sorted cells were then prepared for scRNA-seq using the 10X v3.1 3' GEX droplet-based sc/snRNA-seq platform. cDNA and sequencing libraries were prepared according to the manufacturer's protocols, and then sequenced to a depth of approx 125k read pairs per cell. Additionally, cDNA libraries were amplified with custom PCR primers to maximize detection of AAV-encoded barcodes.  GEX and AAV Barcode libraries were each sequenced.

tag: cell type


title: Nuclei_AiP12805_SYFP2neg_STR_GEX

description:
Library name: Load1158D_NW-TX1067-6
YFP-non-expressing nuclei sorted from nuclei suspensions using FACS. These sorted nuclei were then prepared for scRNA-seq using the 10X v3.1 3' GEX droplet-based sc/snRNA-seq platform. cDNA and sequencing libraries were prepared according to the manufacturer's protocols, and then sequenced to a depth of approx 125k read pairs per cell.

source_name: striatal single nuclei

value: striatal single nuclei

value: sorted YFP-non-expressing single nucleus

value: AiP12805 PHP.eB


title: WholeCells_6plexIndividual_SYFP2pos_STR_GEX

description:
Library name: Load1249C_NW-TX4009-4
YFP-expressing whole cells sorted from cell suspensions using FACS. These sorted cells were then prepared for scRNA-seq using the 10X v3.1 3' GEX droplet-based sc/snRNA-seq platform. cDNA and sequencing libraries were prepared according to the manufacturer's protocols, and then sequenced to a depth of approx 125k read pairs per cell. Additionally, cDNA libraries were amplified with custom PCR primers to maximize detection of AAV-encoded barcodes.  GEX and AAV Barcode libraries were each sequenced.


title: WholeCells_6plexIndividual_SYFP2pos_STR_mBC

description:
Library name: Load1249C_NW-TX4009-4_mBC
YFP-expressing whole cells sorted from cell suspensions using FACS. These sorted cells were then prepared for scRNA-seq using the 10X v3.1 3' GEX droplet-based sc/snRNA-seq platform. cDNA and sequencing libraries were prepared according to the manufacturer's protocols, and then sequenced to a depth of approx 125k read pairs per cell. Additionally, cDNA libraries were amplified with custom PCR primers to maximize detection of AAV-encoded barcodes.  GEX and AAV Barcode libraries were each sequenced.


title: WholeCells_20plex-singlescQers_SYFP2pos_ALM_mBC

description:
Library name: Load3066A_mBC
YFP-expressing whole cells sorted from C57Bl/6 cell suspensions using FACS. These sorted cells were then prepared for scRNA-seq using the 10X v3.1 3' GEX droplet-based sc/snRNA-seq platform. cDNA and sequencing libraries were prepared according to the manufacturer's protocols, and then sequenced to a depth of approx 125k read pairs per cell. Additionally, cDNA libraries were amplified with custom PCR primers to maximize detection of AAV-encoded mBC barcodes, and supernatant from cDNA purification was amplified with different custom PCR primers to detect AAV-encoded oBC barcodes.  GEX and mBC barcode and oBC barcode libraries were each sequenced.


title: Nuclei_AiP12805_SYFP2neg_ALM_GEX

description:
Library name: Load1158B_NW-TX1067-4
YFP-non-expressing nuclei sorted from nuclei suspensions using FACS. These sorted nuclei were then prepared for scRNA-seq using the 10X v3.1 3' GEX droplet-based sc/snRNA-seq platform. cDNA and sequencing libraries were prepared according to the manufacturer's protocols, and then sequenced to a depth of approx 125k read pairs per cell.

source_name: anterior lateral motor cortex single nuclei


title: WholeCells_AiP12805_SYFP2neg_STR_GEX

description:
Library name: Load1141D_NW-TX1065-7
YFP-non-expressing whole cells sorted from cell suspensions using FACS. These sorted cells were then prepared for scRNA-seq using the 10X v3.1 3' GEX droplet-based sc/snRNA-seq platform. cDNA and sequencing libraries were prepared according to the manufacturer's protocols, and then sequenced to a depth of approx 125k read pairs per cell.

value: sorted YFP-non-expressing whole single cell


title: Nuclei_AiP12805_SYFP2pos_STR_GEX

description:
Library name: Load1158C_NW-TX1067-5
YFP-expressing nuclei sorted from nuclei suspensions using FACS. These sorted nuclei were then prepared for scRNA-seq using the 10X v3.1 3' GEX droplet-based sc/snRNA-seq platform. cDNA and sequencing libraries were prepared according to the manufacturer's protocols, and then sequenced to a depth of approx 125k read pairs per cell.

value: sorted YFP-expressing single nucleus


title: WholeCells_AiP12805_SYFP2pos_STR_GEX

description:
Library name: Load1141C_NW-TX1065-6
YFP-expressing whole cells sorted from cell suspensions using FACS. These sorted cells were then prepared for scRNA-seq using the 10X v3.1 3' GEX droplet-based sc/snRNA-seq platform. cDNA and sequencing libraries were prepared according to the manufacturer's protocols, and then sequenced to a depth of approx 125k read pairs per cell.


title: WholeCells_6plexIndividual_SYFP2pos_ALM_GEX

description:
Library name: Load1249A_NW-TX4009-2
YFP-expressing whole cells sorted from cell suspensions using FACS. These sorted cells were then prepared for scRNA-seq using the 10X v3.1 3' GEX droplet-based sc/snRNA-seq platform. cDNA and sequencing libraries were prepared according to the manufacturer's protocols, and then sequenced to a depth of approx 125k read pairs per cell. Additionally, cDNA libraries were amplified with custom PCR primers to maximize detection of AAV-encoded barcodes.  GEX and AAV Barcode libraries were each sequenced.


title: WholeCells_AiP12805_SYFP2pos_ALM_GEX

description:
Library name: Load1141A_NW-TX1065-4
YFP-expressing whole cells sorted from cell suspensions using FACS. These sorted cells were then prepared for scRNA-seq using the 10X v3.1 3' GEX droplet-based sc/snRNA-seq platform. cDNA and sequencing libraries were prepared according to the manufacturer's protocols, and then sequenced to a depth of approx 125k read pairs per cell.


title: C57Bl/6 YFP+ STR single nucleus AiE0391h-H2B-SYFP2-BC-36

description:
Library name: AB-S40331_E1-50_TTCGTTCACA-CTGATTAGTG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.

data_processing:
Illumina NovaSeq 6000 on-board base-calling protocol
Alignment with STAR v2.7.2a (https://github.com/alexdobin/STAR)
Assembly: mm10
Supplementary files format and content: Nuclei_SSv4_GEX_2501.csv.gz (for all three datasets; 2501 samples)

source_name: striatum nucleus

extract_protocol:
SMART-seq v4 protocol (Takara)
Nextera XT DNA Library Preparation Kit (Illumina)

value: striatum nucleus

value: sorted YFP-expressing nucleus

value: AiP14058 PHP.eB


title: C57Bl/6 YFP+ STR single nucleus AiE0391h-H2B-SYFP2-BC-37

description:
Library name: AB-S40331_E1-50_GCGATGCAGA-TCTTCGTGTA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0391h-H2B-SYFP2-BC-35

description:
Library name: AB-S40331_E1-50_CTCATCATCT-TAAGCCTCTA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0391h-H2B-SYFP2-BC-39

description:
Library name: AB-S40331_E1-50_TGCGTTCTGC-TTGGTCGCTC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0391h-H2B-SYFP2-BC-38

description:
Library name: AB-S40331_E1-50_GTACTAAGAG-CGCTCTAGAT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0401h-H2B-SYFP2-BC-40

description:
Library name: AB-S40331_E1-50_ACGACAATGA-AACGCGGTAG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.

value: AiP14057 PHP.eB


title: C57Bl/6 YFP+ STR single nucleus AiE0391h-H2B-SYFP2-BC-33

description:
Library name: AB-S40331_E1-50_CACGTAAGAG-TCAATCCGCG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0391h-H2B-SYFP2-BC-34

description:
Library name: AB-S40331_E1-50_GTACAGCGGA-CAATCTGTGT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0401h-H2B-SYFP2-BC-36

description:
Library name: AB-S40331_E1-50_GCAGATGTAA-TACACGCCTC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0391h-H2B-SYFP2-BC-32

description:
Library name: AB-S40331_E1-50_TCTTAGGTGG-GATCACTGGT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0401h-H2B-SYFP2-BC-39

description:
Library name: AB-S40331_E1-50_TCCAGCGAAG-GTTCTATGTC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0401h-H2B-SYFP2-BC-38

description:
Library name: AB-S40331_E1-50_ACGTGCATCG-CAGTCAGTTG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: Nuclei_AiP12805_SYFP2pos_ALM_GEX

description:
Library name: Load1158A_NW-TX1067-3
YFP-expressing nuclei sorted from nuclei suspensions using FACS. These sorted nuclei were then prepared for scRNA-seq using the 10X v3.1 3' GEX droplet-based sc/snRNA-seq platform. cDNA and sequencing libraries were prepared according to the manufacturer's protocols, and then sequenced to a depth of approx 125k read pairs per cell.

value: anterior lateral motor cortex single nuclei


title: C57Bl/6 YFP+ STR single nucleus AiE0401h-H2B-SYFP2-BC-34

description:
Library name: AB-S40331_E1-50_ACGGCCTACA-TCTCACTCTA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0401h-H2B-SYFP2-BC-33

description:
Library name: AB-S40331_E1-50_CGTCTAGTAA-TTAATCCAGC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0401h-H2B-SYFP2-BC-35

description:
Library name: AB-S40331_E1-50_AACCACACTA-CCTCTGCTGA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0401h-H2B-SYFP2-BC-31

description:
Library name: AB-S40331_E1-50_TCGGAGACCT-AAGGTTACTC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0401h-H2B-SYFP2-BC-29

description:
Library name: AB-S40331_E1-50_TATCCAGTTC-TGAGCTACGT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0401h-H2B-SYFP2-BC-28

description:
Library name: AB-S40331_E1-50_CCTCTGTCAT-CGTATCGATT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0401h-H2B-SYFP2-BC-32

description:
Library name: AB-S40331_E1-50_AAGGTACCGT-CTGATCTTGT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0401h-H2B-SYFP2-BC-37

description:
Library name: AB-S40331_E1-50_AGTCGACGTA-CCATGGAACA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: WholeCells_AiP12805_SYFP2neg_ALM_GEX

description:
Library name: Load1141B_NW-TX1065-5
YFP-non-expressing whole cells sorted from cell suspensions using FACS. These sorted cells were then prepared for scRNA-seq using the 10X v3.1 3' GEX droplet-based sc/snRNA-seq platform. cDNA and sequencing libraries were prepared according to the manufacturer's protocols, and then sequenced to a depth of approx 125k read pairs per cell.


title: C57Bl/6 YFP+ STR single nucleus AiE0401h-H2B-SYFP2-BC-27

description:
Library name: AB-S40331_E1-50_CTTCGGAATC-CGTCGGTAAG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0401h-H2B-SYFP2-BC-25

description:
Library name: AB-S40331_E1-50_TATCATGTCG-TAATCTGCAC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0401h-H2B-SYFP2-BC-30

description:
Library name: AB-S40331_E1-50_GCATAGCCAA-TGGTTCCTGG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus DLX2.0-H2B-SYFP2-BC-39

description:
Library name: AB-S40331_E1-50_TTAAGTGCGT-ACCTGTCGCT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.

value: AiP13968 PHP.eB


title: C57Bl/6 YFP+ STR single nucleus DLX2.0-H2B-SYFP2-BC-37

description:
Library name: AB-S40331_E1-50_AGTGGTAGCA-TGGTGTTGAC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus DLX2.0-H2B-SYFP2-BC-34

description:
Library name: AB-S40331_E1-50_TGTTCGCTCT-ACTTGCGTGT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus DLX2.0-H2B-SYFP2-BC-35

description:
Library name: AB-S40331_E1-50_CATTGAGCTA-ACTTCACATC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus DLX2.0-H2B-SYFP2-BC-33

description:
Library name: AB-S40331_E1-50_ACCTTCGGAC-GACCGTAGAT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus DLX2.0-H2B-SYFP2-BC-36

description:
Library name: AB-S40331_E1-50_CTGAGCTTGT-CTTCTTGCGT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0391h-H2B-SYFP2-BC-30

description:
Library name: AB-S40331_E1-50_TTGGCTCCAA-GTCTGATCCG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0391h-H2B-SYFP2-BC-29

description:
Library name: AB-S40331_E1-50_CCACTTGGAT-AATCTCGGTT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0391h-H2B-SYFP2-BC-31

description:
Library name: AB-S40331_E1-50_TAAGGCGATC-GTTAGCACAA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0401h-H2B-SYFP2-BC-20

description:
Library name: AB-S40331_E1-50_GCGAGATGGA-ACACTCTACT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0401h-H2B-SYFP2-BC-26

description:
Library name: AB-S40331_E1-50_AGGTATTCGG-AGATGTGCGC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0391h-H2B-SYFP2-BC-28

description:
Library name: AB-S40331_E1-50_GTCATCGCGT-CCGACAAGGA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0391h-H2B-SYFP2-BC-26

description:
Library name: AB-S40331_E1-50_CATTATCGCT-CGAGTTGCTT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus DLX2.0-H2B-SYFP2-BC-40

description:
Library name: AB-S40331_E1-50_AATCCAGGAT-CATGCAGAGT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0401h-H2B-SYFP2-BC-24

description:
Library name: AB-S40331_E1-50_GATCAACAAG-AAGCACTGTC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0401h-H2B-SYFP2-BC-18

description:
Library name: AB-S40331_E1-50_AACCGGCCTT-TGTTCAGATC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0391h-H2B-SYFP2-BC-27

description:
Library name: AB-S40331_E1-50_GTCGACTCCT-TGCCGTTAGC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0391h-H2B-SYFP2-BC-25

description:
Library name: AB-S40331_E1-50_TGACAAGAGG-AATCTGTTGG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus DLX2.0-H2B-SYFP2-BC-38

description:
Library name: AB-S40331_E1-50_TCAGACTGGT-TCGATTACGC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0401h-H2B-SYFP2-BC-17

description:
Library name: AB-S40331_E1-50_CTGGAGAATA-TCCATACAAC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0401h-H2B-SYFP2-BC-23

description:
Library name: AB-S40331_E1-50_TGATTCCACA-CTCTAGCCGT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0401h-H2B-SYFP2-BC-21

description:
Library name: AB-S40331_E1-50_AACAGTGACT-ATCGCAGTGT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0387m-H2B-SYFP2-BC-40

description:
Library name: AB-S40331_E1-50_ACTTAGATCG-CGGAATTCTT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.

value: AiP14056 PHP.eB


title: C57Bl/6 YFP+ STR single nucleus AiE0387m-H2B-SYFP2-BC-39

description:
Library name: AB-S40331_E1-50_CAACAGGTAG-GACGATAGTG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0401h-H2B-SYFP2-BC-19

description:
Library name: AB-S40331_E1-50_TTGTGAAGGC-TCGTCAGTAC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0387m-H2B-SYFP2-BC-38

description:
Library name: AB-S40331_E1-50_GTGGAGGTAG-ATCGCGTCAC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0387m-H2B-SYFP2-BC-37

description:
Library name: AB-S40331_E1-50_CTCATTCTGC-TTGAAGCGGT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0387m-H2B-SYFP2-BC-35

description:
Library name: AB-S40331_E1-50_GCCAAGTAAC-TGCGTGATGA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0387m-H2B-SYFP2-BC-36

description:
Library name: AB-S40331_E1-50_AACGACATCT-GTCTTGGAAT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0387m-H2B-SYFP2-BC-32

description:
Library name: AB-S40331_E1-50_AGAGTATGTG-AGATGGCTTC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0387m-H2B-SYFP2-BC-33

description:
Library name: AB-S40331_E1-50_GTGAGAGTAT-ATCTGTGAGG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0387m-H2B-SYFP2-BC-30

description:
Library name: AB-S40331_E1-50_TATGATGCAG-AGGCCAGTTG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0387m-H2B-SYFP2-BC-31

description:
Library name: AB-S40331_E1-50_CGTGGCAGAA-TCTTCCGATG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0387m-H2B-SYFP2-BC-34

description:
Library name: AB-S40331_E1-50_TTGCAATGCG-CGAGACTTCA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0387m-H2B-SYFP2-BC-28

description:
Library name: AB-S40331_E1-50_TGGCACGAAC-CCTGACTCTT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0387m-H2B-SYFP2-BC-29

description:
Library name: AB-S40331_E1-50_CACACTGTTG-TGCATTGGTT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0401h-H2B-SYFP2-BC-22

description:
Library name: AB-S40331_E1-50_AGAGGCACGA-ACGTGGAATT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0387m-H2B-SYFP2-BC-26

description:
Library name: AB-S40331_E1-50_AACATAAGGC-GTTAGCTTAG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0387m-H2B-SYFP2-BC-25

description:
Library name: AB-S40331_E1-50_TTCTACCGGC-TACTCGTAAG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0387m-H2B-SYFP2-BC-22

description:
Library name: AB-S40331_E1-50_CTCAGAATTC-ACAGAGACCG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0387m-H2B-SYFP2-BC-24

description:
Library name: AB-S40331_E1-50_ACAAGAACCT-CGATACTGAA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0387m-H2B-SYFP2-BC-20

description:
Library name: AB-S40331_E1-50_CTTGCGAAGA-AATGACGCGG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0387m-H2B-SYFP2-BC-18

description:
Library name: AB-S40331_E1-50_TCAATCAGAG-GACTCTGGTT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0410m-H2B-SYFP2-BC-36

description:
Library name: AB-S40331_E1-50_TCCTACGTGC-TAGGTGCTAT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.

value: AiP12820 PHP.eB


title: C57Bl/6 YFP+ STR single nucleus AiE0410m-H2B-SYFP2-BC-38

description:
Library name: AB-S40331_E1-50_AGAACCTACA-AGTGTACGGT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0387m-H2B-SYFP2-BC-21

description:
Library name: AB-S40331_E1-50_AGAACCGGAC-TCCTACCATA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0387m-H2B-SYFP2-BC-27

description:
Library name: AB-S40331_E1-50_GCTACAGGTC-TTATGCAGGA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0410m-H2B-SYFP2-BC-34

description:
Library name: AB-S40331_E1-50_AGGCTGGAGA-CAACTGTCAT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0410m-H2B-SYFP2-BC-39

description:
Library name: AB-S40331_E1-50_ATACACAGAG-AGTCAGAAGG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0410m-H2B-SYFP2-BC-33

description:
Library name: AB-S40331_E1-50_AAGAGTACGA-GCAAGGACGT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0387m-H2B-SYFP2-BC-17

description:
Library name: AB-S40331_E1-50_GACAATTAGC-CCGGTCATGA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0387m-H2B-SYFP2-BC-19

description:
Library name: AB-S40331_E1-50_AGGATACGGT-AACCACGTCA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0410m-H2B-SYFP2-BC-32

description:
Library name: AB-S40331_E1-50_GTAGGCCGTT-GCCACTGTCT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0410m-H2B-SYFP2-BC-40

description:
Library name: AB-S40331_E1-50_ACTGGCAAGA-TGTTGTCCAT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0410m-H2B-SYFP2-BC-35

description:
Library name: AB-S40331_E1-50_TGGACAATAG-GAGTCCTGTG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0387m-H2B-SYFP2-BC-23

description:
Library name: AB-S40331_E1-50_ACCTCATTGA-TATCCGATCT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0410m-H2B-SYFP2-BC-37

description:
Library name: AB-S40331_E1-50_AAGTGGCATT-AGAACAATCG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0410m-H2B-SYFP2-BC-29

description:
Library name: AB-S40331_E1-50_GACAATGCGG-TTGTTGAGTG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0410m-H2B-SYFP2-BC-25

description:
Library name: AB-S40331_E1-50_TCGACGTTGG-GATAAGTGTG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0410m-H2B-SYFP2-BC-26

description:
Library name: AB-S40331_E1-50_AATCGAACTC-ACGAACCTTA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0410m-H2B-SYFP2-BC-31

description:
Library name: AB-S40331_E1-50_GTCTTCTCGA-TGGTGCCAAC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0410m-H2B-SYFP2-BC-28

description:
Library name: AB-S40331_E1-50_CGAGTTCGCA-CCGGATTATG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0410m-H2B-SYFP2-BC-24

description:
Library name: AB-S40331_E1-50_CAACCATACA-ACCGGTTATA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0410m-H2B-SYFP2-BC-23

description:
Library name: AB-S40331_E1-50_AGATGTAAGG-CTGTACCAGG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0410m-H2B-SYFP2-BC-27

description:
Library name: AB-S40331_E1-50_ACTTCAACAG-GCGTGAGCAT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0410m-H2B-SYFP2-BC-30

description:
Library name: AB-S40331_E1-50_GTGCTATCGA-TTAAGCACCG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0410m-H2B-SYFP2-BC-20

description:
Library name: AB-S40331_E1-50_TACGCTACTT-TTAGCGTCTT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0410m-H2B-SYFP2-BC-18

description:
Library name: AB-S40331_E1-50_CTAACCTCAA-TCACAGTTGC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0410m-H2B-SYFP2-BC-19

description:
Library name: AB-S40331_E1-50_ATATTCTCGG-AGATGCTCCG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0390m-H2B-SYFP2-BC-40

description:
Library name: AB-S40331_E1-50_TCTCACCAGG-TTAACAGGTC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.

value: AiP12818 PHP.eB


title: C57Bl/6 YFP+ STR single nucleus AiE0410m-H2B-SYFP2-BC-17

description:
Library name: AB-S40331_E1-50_ACCGAATAGA-TCAGCATCCG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0390m-H2B-SYFP2-BC-38

description:
Library name: AB-S40331_E1-50_CCGATAGTAG-GAGTTGCATA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0390m-H2B-SYFP2-BC-32

description:
Library name: AB-S40331_E1-50_CTTGCTATTC-TAGAGTCACG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0390m-H2B-SYFP2-BC-34

description:
Library name: AB-S40331_E1-50_TCAGTTCGAT-CTGAGTCCTT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0410m-H2B-SYFP2-BC-21

description:
Library name: AB-S40331_E1-50_ATGACAGCAC-CGCTTGTTAT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0390m-H2B-SYFP2-BC-33

description:
Library name: AB-S40331_E1-50_CCACAGCCTT-GCTATTGAAG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0390m-H2B-SYFP2-BC-35

description:
Library name: AB-S40331_E1-50_AGCAATTCAC-TCATCGTCTG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0390m-H2B-SYFP2-BC-39

description:
Library name: AB-S40331_E1-50_CTATAACGGA-CGTGACATTC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0390m-H2B-SYFP2-BC-30

description:
Library name: AB-S40331_E1-50_GAGCAGCTTG-TGCCAGTATT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0390m-H2B-SYFP2-BC-31

description:
Library name: AB-S40331_E1-50_CGGAACACAA-AACTCTACCG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0410m-H2B-SYFP2-BC-22

description:
Library name: AB-S40331_E1-50_GTGTCTACAG-ACACTAGCAT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0390m-H2B-SYFP2-BC-27

description:
Library name: AB-S40331_E1-50_CTGGATTACT-AAGTGTGCTA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0390m-H2B-SYFP2-BC-28

description:
Library name: AB-S40331_E1-50_AGACTAGATG-CGTAACACTA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0390m-H2B-SYFP2-BC-26

description:
Library name: AB-S40331_E1-50_TCGACGGCAA-GTAGTTGTGT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0390m-H2B-SYFP2-BC-24

description:
Library name: AB-S40331_E1-50_CGCACTTCGT-AATATGCCAG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0390m-H2B-SYFP2-BC-23

description:
Library name: AB-S40331_E1-50_TCAATTCCGA-TGTAGATCCG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0390m-H2B-SYFP2-BC-37

description:
Library name: AB-S40331_E1-50_GTTGCATCAA-GTTGATGCGA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0390m-H2B-SYFP2-BC-29

description:
Library name: AB-S40331_E1-50_TCAGCATCTA-CCAGTGTACG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0390m-H2B-SYFP2-BC-36

description:
Library name: AB-S40331_E1-50_CCTCTATAGA-GTACTTCCAA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0390m-H2B-SYFP2-BC-21

description:
Library name: AB-S40331_E1-50_CACTTACGGC-GCTGGAGAAG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0390m-H2B-SYFP2-BC-19

description:
Library name: AB-S40331_E1-50_CGAGCGTAGT-TGCTCACGCA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0390m-H2B-SYFP2-BC-22

description:
Library name: AB-S40331_E1-50_AGAACTAACG-TTATCGAGAG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0390m-H2B-SYFP2-BC-18

description:
Library name: AB-S40331_E1-50_CAAGACTCAA-CACCGTCATA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0390m-H2B-SYFP2-BC-17

description:
Library name: AB-S40331_E1-50_TGGAGATCGA-CAGCGGTATG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus AiE0390m-H2B-SYFP2-BC-25

description:
Library name: AB-S40331_E1-50_CCAGACCGAT-GCTACTTGCA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus hI56i-H2B-SYFP2-BC-38

description:
Library name: AB-S40331_E1-50_CCGTGAGTTC-CTTGGTATGA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.

value: AiP14059 PHP.eB


title: C57Bl/6 YFP+ STR single nucleus AiE0390m-H2B-SYFP2-BC-20

description:
Library name: AB-S40331_E1-50_AACAACACAG-GAACATTGGC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus hI56i-H2B-SYFP2-BC-36

description:
Library name: AB-S40331_E1-50_CTCTATTGAG-CACGCAAGTC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus hI56i-H2B-SYFP2-BC-37

description:
Library name: AB-S40331_E1-50_CCGAACAGAG-TGATACTCGC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus hI56i-H2B-SYFP2-BC-39

description:
Library name: AB-S40331_E1-50_AGGTAACAAC-GCACGATGCT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus hI56i-H2B-SYFP2-BC-32

description:
Library name: AB-S40331_E1-50_TCGCGACACT-CGAGTAATGT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus hI56i-H2B-SYFP2-BC-34

description:
Library name: AB-S40331_E1-50_CCTGACATCT-TAGCTACCAG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus hI56i-H2B-SYFP2-BC-33

description:
Library name: AB-S40331_E1-50_CTTACGAACT-ACGGACTGAG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus hI56i-H2B-SYFP2-BC-29

description:
Library name: AB-S40331_E1-50_AGTAAGGAGA-ATGCTTGGTC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus hI56i-H2B-SYFP2-BC-24

description:
Library name: AB-S40331_E1-50_TTCTCTTAGC-TCCTCGAGCT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus hI56i-H2B-SYFP2-BC-27

description:
Library name: AB-S40331_E1-50_ACTTCCGGCA-GACCAATTGT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus hI56i-H2B-SYFP2-BC-31

description:
Library name: AB-S40331_E1-50_AGATTAGCGT-CGCCTTATAT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus hI56i-H2B-SYFP2-BC-23

description:
Library name: AB-S40331_E1-50_TCTAGCTGCG-ATAGGTAAGG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus hI56i-H2B-SYFP2-BC-30

description:
Library name: AB-S40331_E1-50_AGAAGACCTA-AACCGTAACA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus hI56i-H2B-SYFP2-BC-25

description:
Library name: AB-S40331_E1-50_GCCAGCTATC-TTGTTCGTGT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus hI56i-H2B-SYFP2-BC-28

description:
Library name: AB-S40331_E1-50_GCAATCTGCT-ACGAACCAAG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus hI56i-H2B-SYFP2-BC-22

description:
Library name: AB-S40331_E1-50_CCAGTCGTCA-CGCTAGGCTA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus hI56i-H2B-SYFP2-BC-20

description:
Library name: AB-S40331_E1-50_GTGCTCTAGG-GTTACCAACG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus hI56i-H2B-SYFP2-BC-35

description:
Library name: AB-S40331_E1-50_AAGCGCTTAG-TGTCTGTGAC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus DLX2.0-H2B-SYFP2-BC-32

description:
Library name: AB-S40331_E1-50_TTACGCGCAT-CTTAACGACA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus hI56i-H2B-SYFP2-BC-18

description:
Library name: AB-S40331_E1-50_AGTTCAGGCG-CACAGCTATT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus hI56i-H2B-SYFP2-BC-17

description:
Library name: AB-S40331_E1-50_AGGATGTCCA-ACTGGACGTT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus hI56i-H2B-SYFP2-BC-21

description:
Library name: AB-S40331_E1-50_TAGGCGAATG-AGTCCAGTGA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus DLX2.0-H2B-SYFP2-BC-30

description:
Library name: AB-S40331_E1-50_TATGCGTAGC-TCAGAGGCAC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus hI56i-H2B-SYFP2-BC-26

description:
Library name: AB-S40331_E1-50_CGCCAAGGTA-GATAGTGTTC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus DLX2.0-H2B-SYFP2-BC-29

description:
Library name: AB-S40331_E1-50_CCTCCTGTGA-CACGTCTACG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus DLX2.0-H2B-SYFP2-BC-31

description:
Library name: AB-S40331_E1-50_CAGCAGGTCA-CACTTAGGTA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus hI56i-H2B-SYFP2-BC-19

description:
Library name: AB-S40331_E1-50_TCACCATAAG-AAGTTCAGGA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus DLX2.0-H2B-SYFP2-BC-27

description:
Library name: AB-S40331_E1-50_GAGATGAGAG-GAATGGCGTT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus DLX2.0-H2B-SYFP2-BC-28

description:
Library name: AB-S40331_E1-50_TGACACGATT-GCCTAGGAAG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus DLX2.0-H2B-SYFP2-BC-23

description:
Library name: AB-S40331_E1-50_ATGTTGCCAC-TAGAAGCCAT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus DLX2.0-H2B-SYFP2-BC-26

description:
Library name: AB-S40331_E1-50_AATCTGCAGT-TGCGAAGTGA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus DLX2.0-H2B-SYFP2-BC-20

description:
Library name: AB-S40331_E1-50_CTCCGCCATA-GCGTTCAGCA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus DLX2.0-H2B-SYFP2-BC-25

description:
Library name: AB-S40331_E1-50_TATCCGCGTC-GTTGCATCGT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus DLX2.0-H2B-SYFP2-BC-21

description:
Library name: AB-S40331_E1-50_AACGAAGATC-TGTGGTCTAA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus DLX2.0-H2B-SYFP2-BC-19

description:
Library name: AB-S40331_E1-50_CGATGTCTTG-CCGAACGTTC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus DLX2.0-H2B-SYFP2-BC-22

description:
Library name: AB-S40331_E1-50_AATAGGTAGG-TGGCTTATGG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus DLX2.0-H2B-SYFP2-BC-18

description:
Library name: AB-S40331_E1-50_ACTCCGAAGC-TCCGTGTTGC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus DLX2.0-H2B-SYFP2-BC-24

description:
Library name: AB-S40331_E1-50_ACAACAGGCT-TCCACTGTTG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus DLX2.0-H2B-SYFP2-BC-12

description:
Library name: AB-S40331_E1-50_GCCACCTAGT-TGCGATGTAT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus DLX2.0-H2B-SYFP2-BC-17

description:
Library name: AB-S40331_E1-50_CGCGGTATCT-ATTACCGATC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus DLX2.0-H2B-SYFP2-BC-16

description:
Library name: AB-S40331_E1-50_GAACCGCATT-CATTATGCCT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus DLX2.0-H2B-SYFP2-BC-10

description:
Library name: AB-S40331_E1-50_TATGGCAAGC-AACAGCAATG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus DLX2.0-H2B-SYFP2-BC-11

description:
Library name: AB-S40331_E1-50_CCTAAGCTGA-CGTCGCATCT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus DLX2.0-H2B-SYFP2-BC-9

description:
Library name: AB-S40331_E1-50_ATTCGGCTTA-TTAACGTCCG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus CMV-H2B-SYFP2-BC-39

description:
Library name: AB-S40331_E1-50_ATAAGCGGAG-CTGTTCTACG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.

value: AiP13967 PHP.eB


title: C57Bl/6 YFP+ STR single nucleus DLX2.0-H2B-SYFP2-BC-13

description:
Library name: AB-S40331_E1-50_AGTATCCGAT-TATTGCGCTA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus DLX2.0-H2B-SYFP2-BC-14

description:
Library name: AB-S40331_E1-50_CCTCGTTGGA-GACTGCGTTA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus CMV-H2B-SYFP2-BC-38

description:
Library name: AB-S40331_E1-50_GCCAACAGAC-CGTTCACGTT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus CMV-H2B-SYFP2-BC-36

description:
Library name: AB-S40331_E1-50_AACTCTCTAC-ACGGCTTCTC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus CMV-H2B-SYFP2-BC-37

description:
Library name: AB-S40331_E1-50_GTATGCCACA-CCGAGTCTTC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus CMV-H2B-SYFP2-BC-40

description:
Library name: AB-S40331_E1-50_TCCAACTGAA-TGTCCGTAGG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus CMV-H2B-SYFP2-BC-30

description:
Library name: AB-S40331_E1-50_CCATGTAACA-CTCGATCAGG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ STR single nucleus DLX2.0-H2B-SYFP2-BC-15

description:
Library name: AB-S40331_E1-50_CGACCTTAAT-GCTCTTAGGT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.