title: AMB_201117_9mo_5E_6E_4_P2-6-L4-C11


title: AMB_201117_9mo_5E_6E_4_P2-6-L4-B11

status: Public on Dec 17 2025

type: SRA

city: La Jolla

first: Joseph

middle: R

last: Ecker

state: CA

address: 10010 North Torrey Pines Road

department: Genomic Analysis Laboratory

country: USA

institute: HHMI-Salk-Institute

library_source: genomic

data_processing:
Library strategy: snmC-seq3
Reads trimed with Cutadapt for adapters and low quality bases (Phred score >= 20)
The resulting files are mapped to in-silico bisulfite converted reference genome using Bismark.
The raw BAM file will be filtered using samtools to filter reads by MAPQ > 10 and use Picard to remove PCR duplicates
Tab-delimited files (ALLC files) describing the quantity of unmethylated and methylated cytosine base calls will be generated with in-house developing package using the filtered BAM files
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited files (ALLC files) describing the quantity of unmethylated and methylated cytosine base calls. Columns are: 1. Chromosome, 2. Position, 3. Strand, 4. Cytosin Context, 5. methylated base call, 6. total mapped base call, 7. not used, place holder column

molecule: genomic DNA

organism: Mus musculus

extract_protocol:
Regions of mouse brain were dissected.
Library prepared following protocol discribed in Luo, Chongyuan, Angeline Rivkin, Jingtian Zhou, Justin P. Sandoval, Laurie Kurihara, Jacinta Lucero, Rosa Castanon, et al. 2018. “Robust Single-Cell DNA Methylome Profiling with snmC-seq2.” Nature Communications 9 (1): 3824.

tag: strain

tag: age

value: 9 months

tag: brain region

value: CP

tag: gender

value: M


title: AMB_201117_9mo_5E_6E_4_P2-6-L4-B23


title: AMB_201117_9mo_5E_6E_4_P2-6-L4-A23


title: AMB_201117_9mo_5E_6E_4_P2-6-L4-C12


title: AMB_201117_9mo_5E_6E_4_P2-6-L4-E23


title: AMB_201117_9mo_5E_6E_4_P2-6-L4-B12


title: AMB_201117_9mo_5E_6E_4_P2-6-L4-E24


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-P9


title: AMB_201117_9mo_5E_6E_4_P2-6-L4-B24


title: AMB_201117_9mo_5E_6E_4_P2-6-L4-A11


title: AMB_201117_9mo_5E_6E_4_P2-6-L4-A12


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-P22


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-P21


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-O9


title: AMB_201117_9mo_5E_6E_4_P2-6-L4-A24


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-O21


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-O22


title: AMB_201117_9mo_5E_6E_4_P2-6-L4-D23


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-O10


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-P10


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-M9


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-N21


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-M22


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-N9


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-M10


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-M21


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-N10


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-K22


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-L9


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-K9


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-K21


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-N22


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-J9


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-L21


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-L22


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-K10


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-I22


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-I10


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-H9


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-J10


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-J21


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-L10


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-I9


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-H21


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-H10


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-H22


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-I21


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-J22


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-G10


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-G22


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-F10


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-F9


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-E22


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-F21


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-E21


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-D21


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-D22


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-G9


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-D10


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-G21


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-C9


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-B9


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-C22


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-A9


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-C10


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-B21


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-B10


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-D9


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-E9


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-A21


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-C21


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-B22


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-A22


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-P8


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-A10


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-F22


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-P20


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-O8


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-P19


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-P7


title: AMB_201117_9mo_5E_6E_4_P2-5-L10-E10


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-O7


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-O20


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-O19


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-N7


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-M7


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-M8


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-M20


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-N19


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-K19


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-L20


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-L8


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-J7


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-K8


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-M19


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-N20


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-J20


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-J8


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-L19


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-N8


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-K7


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-I19


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-K20


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-H7


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-H8


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-I7


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-J19


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-G8


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-L7


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-G19


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-G20


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-F8


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-G7


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-H19


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-F7


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-H20


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-F19


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-I8


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-E8


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-I20


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-E7


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-F20


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-C8


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-E20


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-C20


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-B8


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-B7


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-B19


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-A19


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-C19


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-E19


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-D7


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-D19


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-P18


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-D20


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-O5


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-B20


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-N6


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-N18


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-A8


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-N5


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-P6


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-L6


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-P5


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-C7


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-A7


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-K5


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-M17


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-L17


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-L5


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-D8


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-O6


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-O18


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-P17


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-L18


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-K17


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-K6


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-K18


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-O17


title: AMB_201117_9mo_5E_6E_4_P2-4-M9-A20


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-J6


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-M5


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-M6


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-I17


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-M18


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-J5


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-J17


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-H17


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-G6


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-I18


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-G18


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-J18


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-F6


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-E6


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-H18


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-I6


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-I5


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-E5


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-G17


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-F17


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-F5


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-E18


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-H6


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-E17


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-F18


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-D6


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-D5


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-H5


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-G5


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-D18


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-C6


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-C5


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-C18


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-B5


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-B6


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-B18


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-C17


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-A5


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-B17


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-P4


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-A6


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-P3


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-O4


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-A18


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-A17


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-O16


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-O3


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-O15


title: AMB_201117_9mo_5E_6E_4_P2-3-L14-D17


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-P15


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-N4


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-N16


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-P16


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-M4


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-N3


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-M3


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-M16


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-M15


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-L4


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-K4


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-N15


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-L3


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-L15


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-L16


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-K3


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-K16


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-J3


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-J15


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-I16


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-J16


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-I3


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-H3


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-H4


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-H16


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-I4


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-K15


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-J4


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-G4


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-G3


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-F4


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-F3


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-F15


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-E15


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-E16


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-I15


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-H15


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-G16


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-F16


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-D3


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-E4


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-D16


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-E3


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-D4


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-G15


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-D15


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-C16


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-C15


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-B16


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-C4


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-B15


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-C3


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-A4


title: AMB_201117_9mo_5E_6E_4_P3-4-K4-P7


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-A3


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-A16


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-B3


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-A15


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-P2


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-P14


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-P13


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-P1


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-O14


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-O2


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-O13


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-N2


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-N13


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-N1


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-M2


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-M1


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-M14


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-O1


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-N14


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-L2


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-M13


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-L14


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-J1


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-K1


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-J14


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-L13


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-L1


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-J2


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-K2


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-K13


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-K14


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-H2


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-I2


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-H1


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-G2


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-I1


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-H14


title: AMB_201117_9mo_5E_6E_4_P2-2-O21-B4


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-F2


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-G13


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-F13


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-I14


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-I13


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-G1


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-J13


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-G14


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-D2


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-D14


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-H13


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-F1


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-C14


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-E14


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-E2


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-F14


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-C13


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-C2


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-E1


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-D13


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-B2


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-E13


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-B1


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-P24


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-A14


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-C1


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-O24


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-A13


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-P12


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-A1


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-D1


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-A2


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-P23


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-B14


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-P11


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-O23


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-N11


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-O11


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-O12


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-M24


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-N24


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-N23


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-M23


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-M12


title: AMB_201117_9mo_5E_6E_4_P2-1-J12-B13


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-K24


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-N12


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-K12


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-M11


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-K23


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-L24


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-L23


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-J11


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-I24


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-L12


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-L11


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-J24


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-I11


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-H11


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-J23


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-I12


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-K11


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-J12


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-H23


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-H24


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-G11


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-H12


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-G23


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-F12


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-F11


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-G24


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-E23


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-G12


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-I23


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-E24


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-D24


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-C23


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-F24


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-F23


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-E11


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-E12


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-D23


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-D11


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-C24


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-D12


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-P22


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-C12


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-P10


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-C11


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-B24


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-A11


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-P9


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-B11


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-B23


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-O9


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-A12


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-A24


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-A23


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-M21


title: AMB_201117_9mo_5E_6E_4_P1-6-M6-B12


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-O10


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-L9


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-N9


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-O22


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-M10


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-O21


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-N22


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-L22


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-K9


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-K21


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-N21


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-N10


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-L10


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-L21


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-M9


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-P21


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-I22


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-I9


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-J10


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-K22


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-K10


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-J22


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-J9


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-M22


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-H9


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-H22


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-J21


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-G21


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-H10


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-I10


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-I21


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-F22


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-G9


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-F9


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-G22


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-H21


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-F21


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-G10


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-F10


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-E9


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-E22


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-E21


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-D9


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-C22


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-D10


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-B10


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-E10


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-A21


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-A9


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-D21


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-C9


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-C10


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-C21


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-B9


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-B22


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-N7


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-A22


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-B21


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-M8


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-O8


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-P8


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-A10


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-O7


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-L8


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-P7


title: AMB_201117_9mo_5E_6E_4_P1-5-D5-D22


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-P20


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-P19


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-K8


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-J8


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-O20


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-N8


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-O19


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-I8


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-N20


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-J19


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-L19


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-I20


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-N19


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-I19


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-M7


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-H7


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-H19


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-H8


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-M19


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-M20


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-G8


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-L7


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-L20


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-K7


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-K20


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-K19


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-J20


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-J7


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-F20


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-E20


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-F19


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-H20


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-I7


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-G7


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-D20


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-C8


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-G20


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-G19


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-B7


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-F7


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-E8


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-F8


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-E7


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-E19


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-C19


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-D8


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-D7


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-A19


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-C7


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-P18


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-P6


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-P5


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-C20


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-D19


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-B8


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-B20


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-B19


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-O17


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-A20


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-A8


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-N17


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-M5


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-O5


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-N18


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-P17


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-O18


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-N5


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-O6


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-N6


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-M18


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-L6


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-L18


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-M6


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-L5


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-K5


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-M17


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-L17


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-K6


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-K17


title: AMB_201117_9mo_5E_6E_4_P1-4-C17-A7


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-K18


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-J5


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-J17


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-I5


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-I6


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-I18


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-J6


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-I17


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-H5


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-H18


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-J18


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-H6


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-G6


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-H17


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-G5


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-G18


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-F6


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-E6


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-F5


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-F18


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-F17


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-D18


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-G17


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-D5


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-E5


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-E18


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-D6


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-C18


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-C5


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-C6


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-B17


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-B6


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-E17


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-B5


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-A5


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-C17


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-P4


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-A18


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-P3


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-P15


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-P16


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-N4


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-O15


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-B18


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-O3


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-A6


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-O4


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-N15


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-M4


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-A17


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-O16


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-M16


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-M3


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-N3


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-N16


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-L3


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-L4


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-M15


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-L16


title: AMB_201117_9mo_5E_6E_4_P1-3-B21-D17


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-K4


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-L15


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-K3


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-K16


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-J4


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-J16


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-I4


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-I15


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-I16


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-I3


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-K15


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-J3


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-H16


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-G4


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-H4


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-G16


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-G3


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-J15


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-H15


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-F16


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-F4


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-F15


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-E3


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-E4


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-E16


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-D4


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-D3


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-D16


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-C4


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-F3


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-E15


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-C16


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-B4


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-C15


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-B3


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-B16


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-A4


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-A15


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-B15


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-D15


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-P1


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-O2


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-P13


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-C3


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-P14


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-A16


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-H3


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-A3


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-O1


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-P2


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-N2


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-O14


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-O13


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-N13


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-M1


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-N14


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-L14


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-L13


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-L2


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-L1


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-K13


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-M2


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-M13


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-N1


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-M14


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-K2


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-J14


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-K1


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-I2


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-H2


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-J13


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-J1


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-J2


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-G2


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-K14


title: AMB_201117_9mo_5E_6E_4_P1-2-B6-G15


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-I14


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-I1


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-I13


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-F13


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-E13


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-H13


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-G13


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-G1


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-D13


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-D1


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-C14


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-H14


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-H1


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-C1


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-F14


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-F2


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-B2


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-B1


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-F1


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-E14


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-B13


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-D2


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-D14


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-G14


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-E1


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-E2


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-C2


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-A13


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-C13


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-A1


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-A14


title: PBMC healthy donor CITE-seq replicate 3-7

status: Public on Dec 12 2025

city: San Francisco

first: Bridget

middle: P

last: Keenan

address: 505 PARNASSUS AVE

institute: University of California San Francisco

description: Library name: HC3-07_FB

library_source: transcriptomic

data_processing:
Cell Ranger version 7.0.0 (10x Genomics, Genome Build: GRCh38 3.0.0) was used to align the raw sequencing data. The feature barcoding library sequences were aligned to a customized reference genome provided by Biolegend containing the oligonucleotide sequences corresponding to each antibody. Cell Ranger generated a single set of barcodes.tsv.gz, features.tsv.gz, and matrix.mtx.gz.
Assembly: GRCm38
Supplementary files format and content: Cell Ranger output filtered matrix files for both scRNA-seq libraries and CITE-seq (feature barcoding) libraries: barcodes.tsv.gz, features.tsv.gz, matrix.mtx.gz.
Library strategy: CITE-seq

molecule: total RNA

organism: Homo sapiens

source_name: Hepatocellular Carcinoma (HCC)

extract_protocol:
Blood samples were processed using Ficoll (Cytiva); after centrifugation, the PBMC layer was isolated and cryopreserved in cell media with human serum and DMSO. Previously frozen PBMCs were thawed using media containing RPMI, heat-inactivated sterile filtered fetal bovine serum, penicillin-streptomycin, non-essential amino acids, sodium pyruvate, and L-glutamine. 1.1x105 cells from each sample were combined into staining buffer (1 mmol/L EDTA and 2% heat-inactivated FBS in calcium/magnesium-free Dulbecco PBS; Thermo Fisher Scientific) and stained with one pooled cocktail containing a custom panel of 197 Total-Seq C antibody–oligonucleotide conjugates (BioLegend) as per standard protocols following pre-incubation with TruStain FcX (Fc Receptor Blocking Solution, Biolegend).
Droplet-based single cell RNA sequencing (scRNAseq) was performed using the 10x Genomics Chromium Single Cell 5ʹ Reagent Kits v2, according to manufacturer instructions. 10X Genomics 5′ v2 Library Construction kits for feature barcoding (protein antibodies) or CITE-seq were used to generate libraries per manufacturer instructions. All sequencing was performed on an Illumina NovaSeq S4 sequencer with paired end 200 base pair read length and 25,000 reads per droplet for gene expression libraries and 5,000 reads per cell for feature barcode libraries.

tag: tissue

value: Hepatocellular Carcinoma (HCC)

tag: cell type

value: peripheral blood mononuclear cells (PBMCs)

tag: genotype

value: HD

tag: treatment

value: healthy donor

tag: time


title: PBMC healthy donor CITE-seq replicate 3-6

description: Library name: HC3-06_FB


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-A2


title: HCC PBMC patient A-36 timepoint C1D1 CITE-seq replicate 1

description: Library name: A-36-01_01_FB

value: durvulamab


title: AMB_201117_9mo_5E_6E_4_P1-1-I24-B14


title: PBMC healthy donor CITE-seq replicate 3-4

description: Library name: HC3-04_FB


title: PBMC healthy donor CITE-seq replicate 3-8

description: Library name: HC3-08_FB


title: HCC PBMC patient A-35 timepoint C1D1 CITE-seq replicate 1

description: Library name: A-35-01_01_FB


title: HCC PBMC patient A-36 timepoint C1D1 CITE-seq replicate 2

description: Library name: A-36-01_02_FB


title: HCC PBMC patient A-33 timepoint C1D1 CITE-seq replicate 3

description: Library name: A-33-01_03_FB

value: durvalumab+tremelimumab


title: HCC PBMC patient A-34 timepoint C1D1 CITE-seq replicate 1

description: Library name: A-34-01_01_FB

value: nivolumab


title: PBMC healthy donor CITE-seq replicate 3-5

description: Library name: HC3-05_FB


title: PBMC healthy donor CITE-seq replicate 3-2

description: Library name: HC3-02_FB


title: HCC PBMC patient A-34 timepoint C1D1 CITE-seq replicate 2

description: Library name: A-34-01_02_FB


title: PBMC healthy donor CITE-seq replicate 2-5

description: Library name: HC2-05_FB


title: HCC PBMC patient A-35 timepoint C1D1 CITE-seq replicate 2

description: Library name: A-35-01_02_FB


title: PBMC healthy donor CITE-seq replicate 2-4

description: Library name: HC2-04_FB


title: HCC PBMC patient B-07 timepoint C2D1 CITE-seq replicate 2

description: Library name: B-07-04_02_FB

value: sorafenib + nivolumab


title: PBMC healthy donor CITE-seq replicate 3-9

description: Library name: HC3-09_FB


title: PBMC healthy donor CITE-seq replicate 3-1

description: Library name: HC3-p1_FB


title: PBMC healthy donor CITE-seq replicate 3-10

description: Library name: HC3-10_FB


title: PBMC healthy donor CITE-seq replicate 2-3

description: Library name: HC2-03_FB


title: HCC PBMC patient A-33 timepoint C1D1 CITE-seq replicate 2

description: Library name: A-33-01_02_FB


title: HCC PBMC patient B-14 timepoint C1D1 CITE-seq replicate 2

description: Library name: B-14-01_02_FB


title: HCC PBMC patient B-14 timepoint C2D1 CITE-seq replicate 2

description: Library name: B-14-03_02_FB


title: HCC PBMC patient B-11 timepoint baseline CITE-seq replicate 2

description: Library name: B-11-01_02_FB

value: no treatment

value: baseline


title: HCC PBMC patient B-06 timepoint C3D1 CITE-seq

description: Library name: B-06-06_FB


title: PBMC healthy donor CITE-seq replicate 2-2

description: Library name: HC2-02_FB


title: HCC PBMC patient B-07 timepoint C1D1 CITE-seq

description: Library name: B-07-02_FB


title: HCC PBMC patient A-05 timepoint C4D1 CITE-seq replicate 2

description: Library name: A-05-01_02_FB


title: PBMC healthy donor CITE-seq replicate 3-3

description: Library name: HC3-03_FB


title: HCC PBMC patient A-31 timepoint C2D1 CITE-seq

description: Library name: A-31-02_FB


title: HCC PBMC patient A-33 timepoint C1D1 CITE-seq replicate 1

description: Library name: A-33-01_01_FB


title: PBMC healthy donor CITE-seq replicate 2-1

description: Library name: HC2-01_FB


title: HCC PBMC patient B-04 timepoint baseline CITE-seq

description: Library name: B-04-01_FB


title: PBMC healthy donor CITE-seq replicate 1-8

description: Library name: HC1-08_FB


title: HCC PBMC patient B-06 timepoint C2D1 CITE-seq

description: Library name: B-06-04_FB


title: HCC PBMC patient B-03 timepoint C2D1 CITE-seq

description: Library name: B-03-04_FB


title: HCC PBMC patient B-21 timepoint C3D1 CITE-seq

description: Library name: B-21-05_FB


title: HCC PBMC patient B-07 timepoint C2D1 CITE-seq replicate 1

description: Library name: B-07-04_01_FB


title: HCC PBMC patient B-22 timepoint C1D1 CITE-seq

description: Library name: B-22-02_FB


title: HCC PBMC patient B-08 timepoint C2D1 CITE-seq

description: Library name: B-08-04_FB


title: HCC PBMC patient B-05 timepoint C2D1 CITE-seq

description: Library name: B-05-04_FB


title: HCC PBMC patient A-03 timepoint Long term follow up CITE-seq replicate 2

description: Library name: A-03-01_02_FB

value: temsirolimus + sorafenib

value: Long term follow up


title: HCC PBMC patient A-08 timepoint C1D1 CITE-seq replicate 2

description: Library name: A-08-01_02_FB


title: HCC PBMC patient B-21 timepoint C2D1 CITE-seq

description: Library name: B-21-03_FB


title: HCC PBMC patient B-08 timepoint Safety follow up CITE-seq

description: Library name: B-08-08_FB

value: Safety follow up


title: HCC PBMC patient B-18 timepoint baseline CITE-seq

description: Library name: B-18-01_FB


title: HCC PBMC patient A-30 timepoint C1D1 CITE-seq replicate 2

description: Library name: A-30-01_02_FB

value: atezolizumab + bevacizumab


title: HCC PBMC patient A-03 timepoint Long term follow up CITE-seq replicate 1

description: Library name: A-03-01_01_FB


title: PBMC healthy donor CITE-seq replicate 1-7

description: Library name: HC1-07_FB


title: HCC PBMC patient B-15 timepoint C1D1 CITE-seq replicate 2

description: Library name: B-15-01_02_FB


title: HCC PBMC patient A-02 timepoint C2D1 CITE-seq

description: Library name: A-02-02_FB


title: HCC PBMC patient B-13 timepoint C1D1 CITE-seq

description: Library name: B-13-01_FB


title: HCC PBMC patient A-02 timepoint C1D1 CITE-seq

description: Library name: A-02-01_FB


title: HCC PBMC patient B-01 timepoint baseline CITE-seq

description: Library name: B-01-01_FB


title: HCC PBMC patient B-15 timepoint C1D1 CITE-seq replicate 1

description: Library name: B-15-01_01_FB


title: HCC PBMC patient B-14 timepoint C1D1 CITE-seq replicate 1

description: Library name: B-14-01_01_FB


title: PBMC healthy donor CITE-seq replicate 1-6

description: Library name: HC1-06_FB


title: HCC PBMC patient B-06 timepoint C1D1 CITE-seq

description: Library name: B-06-02_FB


title: HCC PBMC patient B-17 timepoint C2D1 CITE-seq

description: Library name: B-17-04_FB


title: HCC PBMC patient B-12 timepoint C1D1 CITE-seq

description: Library name: B-12-01_FB


title: HCC PBMC patient B-17 timepoint Safety follow up CITE-seq

description: Library name: B-17-07_FB


title: HCC PBMC patient B-23 timepoint C1D1 CITE-seq

description: Library name: B-23-02_FB


title: HCC PBMC patient B-06 timepoint Safety follow up CITE-seq

description: Library name: B-06-07_FB


title: HCC PBMC patient B-02 timepoint C2D1 CITE-seq

description: Library name: B-02-04_FB


title: HCC PBMC patient B-11 timepoint baseline CITE-seq replicate 1

description: Library name: B-11-01_01_FB


title: HCC PBMC patient A-30 timepoint C1D1 CITE-seq replicate 1

description: Library name: A-30-01_01_FB


title: HCC PBMC patient B-08 timepoint C7D1 CITE-seq

description: Library name: B-08-07_FB


title: HCC PBMC patient B-10 timepoint C1D1 CITE-seq

description: Library name: B-10-01_FB


title: HCC PBMC patient B-23 timepoint C1D15 CITE-seq

description: Library name: B-23-03_FB


title: PBMC healthy donor CITE-seq replicate 1-5

description: Library name: HC1-05_FB


title: HCC PBMC patient B-14 timepoint C2D1 CITE-seq replicate 1

description: Library name: B-14-03_01_FB


title: HCC PBMC patient A-07 timepoint C2D1 CITE-seq replicate 2

description: Library name: A-07-02_FB


title: HCC PBMC patient A-21 timepoint baseline CITE-seq

description: Library name: A-21-01_FB


title: HCC PBMC patient B-03 timepoint C7D1 CITE-seq

description: Library name: B-03-07_FB


title: HCC PBMC patient B-05 timepoint C1D1 CITE-seq

description: Library name: B-05-02_FB


title: HCC PBMC patient B-09 timepoint baseline CITE-seq

description: Library name: B-09-01_FB


title: HCC PBMC patient A-05 timepoint C4D1 CITE-seq replicate 1

description: Library name: A-05-01_01_FB


title: PBMC healthy donor CITE-seq replicate 1-3

description: Library name: HC1-03_FB


title: HCC PBMC patient B-08 timepoint C1D1 CITE-seq

description: Library name: B-08-02_FB


title: HCC PBMC patient A-08 timepoint C1D1 CITE-seq replicate 1

description: Library name: A-08-01_01_FB


title: HCC PBMC patient B-02 timepoint C1D1 CITE-seq

description: Library name: B-02-02_FB


title: HCC PBMC patient A-12 timepoint baseline CITE-seq replicate 2

description: Library name: A-12-01_02_FB


title: HCC PBMC patient B-22 timepoint C2D15 CITE-seq

description: Library name: B-22-04_FB


title: HCC PBMC patient B-13 timepoint C2D1 CITE-seq

description: Library name: B-13-03_FB


title: HCC PBMC patient A-04 timepoint C2D1 CITE-seq replicate 2

description: Library name: A-04-01_02_FB


title: HCC PBMC patient A-12 timepoint baseline CITE-seq replicate 1

description: Library name: A-12-01_01_FB


title: HCC PBMC patient B-12 timepoint C2D1 CITE-seq

description: Library name: B-12-03_FB


title: HCC PBMC patient B-03 timepoint C1D1 CITE-seq

description: Library name: B-03-02_FB


title: HCC PBMC patient A-32 timepoint C1D1 CITE-seq

description: Library name: A-32-01_FB


title: PBMC healthy donor CITE-seq replicate 1-1

description: Library name: HC1-01_FB


title: HCC PBMC patient B-10 timepoint C2D1 CITE-seq

description: Library name: B-10-03_FB


title: HCC PBMC patient A-04 timepoint C2D1 CITE-seq replicate 1

description: Library name: A-04-01_01_FB


title: HCC PBMC patient B-21 timepoint C1D1 CITE-seq

description: Library name: B-21-01_FB


title: HCC PBMC patient B-22 timepoint C3D1 CITE-seq

description: Library name: B-22-05_FB


title: PBMC healthy donor scRNA-seq replicate 3-9

description: Library name: HC3-09_GEX

library_source: transcriptomic single cell

data_processing:
Cell Ranger version 7.0.0 (10x Genomics, Genome Build: GRCh38 3.0.0) was used to align the raw sequencing data. The feature barcoding library sequences were aligned to a customized reference genome provided by Biolegend containing the oligonucleotide sequences corresponding to each antibody. Cell Ranger generated a single set of barcodes.tsv.gz, features.tsv.gz, and matrix.mtx.gz.
Assembly: GRCm38
Supplementary files format and content: Cell Ranger output filtered matrix files for both scRNA-seq libraries and CITE-seq (feature barcoding) libraries: barcodes.tsv.gz, features.tsv.gz, matrix.mtx.gz.


title: HCC PBMC patient B-23 timepoint C3D1 CITE-seq

description: Library name: B-23-04_FB


title: HCC PBMC patient A-36 timepoint C1D1 scRNA-seq replicate 2

description: Library name: A-36-01_02_GEX


title: PBMC healthy donor scRNA-seq replicate 3-7

description: Library name: HC3-07_GEX


title: HCC PBMC patient A-07 timepoint C2D1 CITE-seq replicate 1

description: Library name: A-07-01_FB


title: HCC PBMC patient B-17 timepoint C1D1 CITE-seq

description: Library name: B-17-02_FB


title: PBMC healthy donor scRNA-seq replicate 3-10

description: Library name: HC3-10_GEX


title: PBMC healthy donor scRNA-seq replicate 3-8

description: Library name: HC3-08_GEX


title: HCC PBMC patient A-34 timepoint C1D1 scRNA-seq replicate 2

description: Library name: A-34-01_02_GEX


title: HCC PBMC patient A-35 timepoint C1D1 scRNA-seq replicate 1

description: Library name: A-35-01_01_GEX


title: PBMC healthy donor scRNA-seq replicate 3-5

description: Library name: HC3-05_GEX


title: PBMC healthy donor scRNA-seq replicate 3-6

description: Library name: HC3-06_GEX


title: PBMC healthy donor scRNA-seq replicate 3-3

description: Library name: HC3-03_GEX


title: HCC PBMC patient A-34 timepoint C1D1 scRNA-seq replicate 1

description: Library name: A-34-01_01_GEX


title: HCC PBMC patient A-33 timepoint C1D1 scRNA-seq replicate 3

description: Library name: A-33-01_03_GEX


title: HCC PBMC patient A-36 timepoint C1D1 scRNA-seq replicate 1

description: Library name: A-36-01_01_GEX


title: HCC PBMC patient A-33 timepoint C1D1 scRNA-seq replicate 2

description: Library name: A-33-01_02_GEX


title: PBMC healthy donor scRNA-seq replicate 3-4

description: Library name: HC3-04_GEX


title: HCC PBMC patient A-35 timepoint C1D1 scRNA-seq replicate 2

description: Library name: A-35-01_02_GEX


title: PBMC healthy donor scRNA-seq replicate 3-2

description: Library name: HC3-02_GEX


title: PBMC healthy donor scRNA-seq replicate 3-1

description: Library name: HC3-p1_GEX


title: HCC PBMC patient B-15 timepoint C1D1 scRNA-seq replicate 2

description: Library name: B-15-01_02_GEX


title: HCC PBMC patient B-14 timepoint C2D1 scRNA-seq replicate 2

description: Library name: B-14-03_02_GEX


title: PBMC healthy donor scRNA-seq replicate 2-4

description: Library name: HC2-04_GEX


title: PBMC healthy donor scRNA-seq replicate 2-3

description: Library name: HC2-03_GEX


title: HCC PBMC patient B-07 timepoint C2D1 scRNA-seq replicate 2

description: Library name: B-07-04_02_GEX


title: HCC PBMC patient B-14 timepoint C1D1 scRNA-seq replicate 2

description: Library name: B-14-01_02_GEX


title: HCC PBMC patient B-11 timepoint baseline scRNA-seq replicate 2

description: Library name: B-11-01_02_GEX


title: HCC PBMC patient B-06 timepoint C3D1 scRNA-seq

description: Library name: B-06-06_GEX


title: PBMC healthy donor scRNA-seq replicate 2-2

description: Library name: HC2-02_GEX


title: HCC PBMC patient A-05 timepoint C4D1 scRNA-seq replicate 2

description: Library name: A-05-01_02_GEX


title: HCC PBMC patient B-07 timepoint C1D1 scRNA-seq

description: Library name: B-07-02_GEX


title: HCC PBMC patient A-33 timepoint C1D1 scRNA-seq replicate 1

description: Library name: A-33-01_01_GEX


title: HCC PBMC patient A-08 timepoint C1D1 scRNA-seq replicate 2

description: Library name: A-08-01_02_GEX


title: HCC PBMC patient B-06 timepoint C2D1 scRNA-seq

description: Library name: B-06-04_GEX


title: PBMC healthy donor scRNA-seq replicate 2-5

description: Library name: HC2-05_GEX


title: HCC PBMC patient B-21 timepoint C3D1 scRNA-seq

description: Library name: B-21-05_GEX


title: HCC PBMC patient A-31 timepoint C2D1 scRNA-seq

description: Library name: A-31-02_GEX


title: PBMC healthy donor scRNA-seq replicate 2-1

description: Library name: HC2-01_GEX


title: HCC PBMC patient B-18 timepoint baseline scRNA-seq

description: Library name: B-18-01_GEX


title: HCC PBMC patient B-04 timepoint baseline scRNA-seq

description: Library name: B-04-01_GEX


title: HCC PBMC patient B-22 timepoint C1D1 scRNA-seq

description: Library name: B-22-02_GEX


title: PBMC healthy donor scRNA-seq replicate 1-8

description: Library name: HC1-08_GEX


title: HCC PBMC patient B-08 timepoint C2D1 scRNA-seq

description: Library name: B-08-04_GEX


title: HCC PBMC patient A-02 timepoint C2D1 scRNA-seq

description: Library name: A-02-02_GEX


title: HCC PBMC patient B-14 timepoint C1D1 scRNA-seq replicate 1

description: Library name: B-14-01_01_GEX


title: HCC PBMC patient B-03 timepoint C2D1 scRNA-seq

description: Library name: B-03-04_GEX


title: HCC PBMC patient B-07 timepoint C2D1 scRNA-seq replicate 1

description: Library name: B-07-04_01_GEX


title: HCC PBMC patient B-01 timepoint baseline scRNA-seq

description: Library name: B-01-01_GEX


title: HCC PBMC patient B-21 timepoint C2D1 scRNA-seq

description: Library name: B-21-03_GEX


title: HCC PBMC patient B-05 timepoint C2D1 scRNA-seq

description: Library name: B-05-04_GEX


title: HCC PBMC patient A-03 timepoint Long term follow up scRNA-seq replicate 1

description: Library name: A-03-01_01_GEX


title: HCC PBMC patient B-13 timepoint C1D1 scRNA-seq

description: Library name: B-13-01_GEX


title: HCC PBMC patient A-30 timepoint C1D1 scRNA-seq replicate 2

description: Library name: A-30-01_02_GEX


title: PBMC healthy donor scRNA-seq replicate 1-7

description: Library name: HC1-07_GEX


title: HCC PBMC patient B-15 timepoint C1D1 scRNA-seq replicate 1

description: Library name: B-15-01_01_GEX


title: HCC PBMC patient B-06 timepoint Safety follow up scRNA-seq

description: Library name: B-06-07_GEX


title: HCC PBMC patient A-03 timepoint Long term follow up scRNA-seq replicate 2

description: Library name: A-03-01_02_GEX


title: HCC PBMC patient B-23 timepoint C1D1 scRNA-seq

description: Library name: B-23-02_GEX


title: HCC PBMC patient B-08 timepoint Safety follow up scRNA-seq

description: Library name: B-08-08_GEX


title: HCC PBMC patient B-02 timepoint C2D1 scRNA-seq

description: Library name: B-02-04_GEX


title: PBMC healthy donor scRNA-seq replicate 1-6

description: Library name: HC1-06_GEX


title: HCC PBMC patient B-17 timepoint Safety follow up scRNA-seq

description: Library name: B-17-07_GEX


title: HCC PBMC patient B-06 timepoint C1D1 scRNA-seq

description: Library name: B-06-02_GEX


title: HCC PBMC patient B-08 timepoint C7D1 scRNA-seq

description: Library name: B-08-07_GEX


title: HCC PBMC patient B-12 timepoint C1D1 scRNA-seq

description: Library name: B-12-01_GEX


title: HCC PBMC patient A-30 timepoint C1D1 scRNA-seq replicate 1

description: Library name: A-30-01_01_GEX


title: PBMC healthy donor scRNA-seq replicate 1-5

description: Library name: HC1-05_GEX


title: HCC PBMC patient B-17 timepoint C2D1 scRNA-seq

description: Library name: B-17-04_GEX


title: HCC PBMC patient B-10 timepoint C1D1 scRNA-seq

description: Library name: B-10-01_GEX


title: HCC PBMC patient B-11 timepoint baseline scRNA-seq replicate 1

description: Library name: B-11-01_01_GEX


title: HCC PBMC patient A-02 timepoint C1D1 scRNA-seq

description: Library name: A-02-01_GEX


title: HCC PBMC patient A-05 timepoint C4D1 scRNA-seq replicate 1

description: Library name: A-05-01_01_GEX


title: HCC PBMC patient B-05 timepoint C1D1 scRNA-seq

description: Library name: B-05-02_GEX


title: HCC PBMC patient A-07 timepoint C2D1 scRNA-seq replicate 2

description: Library name: A-07-02_GEX


title: HCC PBMC patient A-21 timepoint baseline scRNA-seq

description: Library name: A-21-01_GEX


title: HCC PBMC patient A-08 timepoint C1D1 scRNA-seq replicate 1

description: Library name: A-08-01_01_GEX


title: HCC PBMC patient B-03 timepoint C7D1 scRNA-seq

description: Library name: B-03-07_GEX


title: HCC PBMC patient B-09 timepoint baseline scRNA-seq

description: Library name: B-09-01_GEX


title: HCC PBMC patient B-12 timepoint C2D1 scRNA-seq

description: Library name: B-12-03_GEX


title: HCC PBMC patient B-13 timepoint C2D1 scRNA-seq

description: Library name: B-13-03_GEX


title: HCC PBMC patient B-14 timepoint C2D1 scRNA-seq replicate 1

description: Library name: B-14-03_01_GEX


title: HCC PBMC patient B-02 timepoint C1D1 scRNA-seq

description: Library name: B-02-02_GEX


title: HCC PBMC patient B-17 timepoint C1D1 scRNA-seq

description: Library name: B-17-02_GEX


title: HCC PBMC patient B-23 timepoint C1D15 scRNA-seq

description: Library name: B-23-03_GEX


title: HCC PBMC patient B-22 timepoint C2D15 scRNA-seq

description: Library name: B-22-04_GEX


title: PBMC healthy donor scRNA-seq replicate 1-3

description: Library name: HC1-03_GEX


title: HCC PBMC patient A-12 timepoint baseline scRNA-seq replicate 2

description: Library name: A-12-01_02_GEX


title: HCC PBMC patient A-04 timepoint C2D1 scRNA-seq replicate 2

description: Library name: A-04-01_02_GEX


title: HCC PBMC patient A-32 timepoint C1D1 scRNA-seq

description: Library name: A-32-01_GEX


title: PBMC healthy donor scRNA-seq replicate 1-1

description: Library name: HC1-01_GEX


title: HCC PBMC patient B-08 timepoint C1D1 scRNA-seq

description: Library name: B-08-02_GEX


title: HCC PBMC patient B-03 timepoint C1D1 scRNA-seq

description: Library name: B-03-02_GEX


title: HCC PBMC patient B-22 timepoint C3D1 scRNA-seq

description: Library name: B-22-05_GEX


title: HCC PBMC patient A-07 timepoint C2D1 scRNA-seq replicate 1

description: Library name: A-07-01_GEX


title: HCC PBMC patient B-21 timepoint C1D1 scRNA-seq

description: Library name: B-21-01_GEX


title: HCC PBMC patient B-10 timepoint C2D1 scRNA-seq

description: Library name: B-10-03_GEX


title: Control_S3

status: Public on Dec 04 2025

city: Seattle

first: Yuzuru

last: Sasamoto

state: WA

address: 750 Republican St

department: Ophthalmology

institute: University of Washington

description: Library name: Sample 7

data_processing:
Adaptor and low-quality sequences were trimmed from the raw reads and the trimmed reads were aligned to the reference genome using the STAR program (version 2.6.1d). Differential expression analyses were performed by the DESeq2 R package (version 1.28.0).
Assembly: hg38
Supplementary files format and content: Matrix table with normalized counts for every gene and every sample

source_name: Cultured corneal epithelial cells

extract_protocol:
Human donor corneas were obtained from eye banks. The central cornea was removed by an 8mm disposable biopsy punch and the corneal endothelium was removed mechanically. After 1 h incubation with PluriSTEM Dispase II Solution at 37 °C, limbal epithelial cells were scraped and incubated with TrypLE Express Enzyme at 37 °C for 30 min to dissociate. Dissociated limbal epithelial cells were cultured and treated with siRNAs (Control, MYEOV knockdown #1, and MYEOV knockdown #2).  Transfection was repeated on day 2 and day 4 and the cells were harvested on day 7 for RNA extraction.
After the removal of rRNA, approximately 500 ng of total RNA underwent library preparation with the Zymo-Seq RiboFree Total RNA Library Prep Kit (Zymo Research). RNA-seq libraries were sequenced on an Illumina NovaSeq to a sequencing depth of at least 30 million read pairs (150 bp paired-end sequencing) per sample.

value: Cultured corneal epithelial cells

value: In vitro human corneal epithelial cells

value: Sample 3, Control


title: HCC PBMC patient B-23 timepoint C3D1 scRNA-seq

description: Library name: B-23-04_GEX

value: Hepatocellular Carcinoma (HCC)


title: MYEOV_KD#2_S3

description: Library name: Sample 9

value: Sample 3, MYEOV knockdown #2


title: MYEOV_KD#2_S2

description: Library name: Sample 6

value: Sample 2, MYEOV knockdown #2


title: HCC PBMC patient A-04 timepoint C2D1 scRNA-seq replicate 1

description: Library name: A-04-01_01_GEX

value: peripheral blood mononuclear cells (PBMCs)


title: HCC PBMC patient A-12 timepoint baseline scRNA-seq replicate 1

description: Library name: A-12-01_01_GEX


title: MYEOV_KD#1_S2

description: Library name: Sample 5

value: Sample 2, MYEOV knockdown #1


title: MYEOV_KD#2_S1

description: Library name: Sample 3

value: Sample 1, MYEOV knockdown #2


title: MYEOV_KD#1_S3

description: Library name: Sample 8

value: Sample 3, MYEOV knockdown #1


title: Control_S2

description: Library name: Sample 4

value: Sample 2, Control


title: Control_S1

description: Library name: Sample 1

value: Sample 1, Control


title: MYEOV_KD#1_S1

description: Library name: Sample 2

value: Sample 1, MYEOV knockdown #1


title: smallRNA-Seq Zeb1_200H_pituitary_3

status: Public on Dec 01 2025

city: Cambridge

first: David

last: Bartel

state: MA

address: 9 Cambridge Center

department: Biology

institute: Whitehead Institute / MIT

description: small RNA

library_source: other

data_processing:
Reads were trimmed of adaptor sequence using cutadapt and filtered using fastq_quality_filter (FastX Toolkit; http://hannonlab.cshl.edu/fastx_toolkit/) with the parameters “-q 30 –p 100” to ensure that all bases had an accuracy of 99.9%. To count the miRNAs in each library, the first 18 nt of each read were string-matched to a dictionary of miRNA sequences. Mouse miRNA dictionaries were based on annotations in miRbase_v20. Matching to the first 18 nt has the advantage of combining all miRNA isoforms into a single count but also can result in ambiguous read assignments for mature miRNAs from distinct loci that are identical for the first 18 nt but differ at one or more positions closer to their 3′ ends (such as let-7a-5p and let-7c-5p). To avoid this ambiguity, these miRNAs (<5% of all annotations) were removed from the dictionary. Differential expression was determined using DESeq2 with the parameter “betaPrior = FALSE”.
Assembly: miRbase_v20
Supplementary files format and content: tab-delimited text file of DESeq2 output includes miRbase name (Feature.ID), baseMean, log2 fold change, lfcSE, stat, p-value, adjusted p-value, raw counts, and normalized counts

molecule: other

source_name: pituitary

extract_protocol:
Total RNA was extracted from mouse tissues using TRI Reagent (ThermoFisher) according to the manufacturer’s protocol with the following modifications. Mouse tissues were rapidly dissected after CO2-based euthanasia and flash frozen in Eppendorf tubes in liquid N2. Tissue was transferred to a 15 mL conical tube, 1–2 mL of TRI Reagent was added, and the tissue was homogenized with a TissueRuptor (Qiagen) and disposable probes. Samples were transferred to Eppendorf tubes (1 mL per tube) and phase separated with 200 μL chloroform (J.T. Baker Analytical). After isopropanol precipitation and two 70% ethanol washes, total RNA was resuspended in water.
Each small-RNA sequencing libraries was prepared with 0.5 μg of total RNA extracted from mouse pituitary. To minimize loss due to low starting input, 3 ug of total RNA from the budding yeast Naumovozyma castelli was added to each sample prior to size-selection. In addition, 0.5 fmoles of miR-427-5p (X. tropicalis) and 0.5 fmoles of miR-14 (D. melanogaster) were added as spike-ins. From each sample, RNAs that co-migrated within the range of 18 and 32 nt radiolabeled internal standards were isolated on a 15% polyacrylamide urea gel. Purified RNA was ligated to a preadenylated 3′ adapter (AppNNNNTCGTATGCCGTCTTCTGCTTGddC) using T4 RNA Ligase 2 KQ mutant (NEB) in a reaction supplemented with 10% polyethylene glycol (PEG 8000, NEB). To reduce ligation biases, this 3′ adapter had 4 random-sequence positions at its 5′ end. After gel purification on a 8% polyacrylamide urea gel, RNA was ligated to a 5′ adapter (GUUCAGAGUUCUACAGUCCGACGAUCNNNN) using T4 RNA Ligase I (NEB) in a reaction supplemented with 10% PEG. To reduce ligation biases, this adapter had 4 random-sequence positions at its 3′ end. After gel purification on an 8% polyacrylamide urea gel, RNA was reverse transcribed with SuperScript III (Invitrogen), and the cDNA was amplified ~12 cycles with KAPA HiFi DNA polymerase (Kapa Biosystems). Amplified DNA was purified on a 3% metaphor agarose gel and submitted for sequencing. A step-by-step protocol for constructing libraries for small-RNA sequencing is available at http://bartellab.wi.mit.edu/protocols.html
small-RNA-seq

value: pituitary

tag: Sex

value: female

value: 28–36 weeks old

tag: background


title: smallRNA-Seq Zeb1_200M_pituitary_1

description: small RNA


title: smallRNA-Seq Zeb1_WT_pituitary_4

description: small RNA


title: smallRNA-Seq Zeb1_WT_pituitary_3

description: small RNA


title: miRNA-Seq, Zeb1_200M_pituitary_3_youngCohort

description:
Library name: Zeb1_200M_pituitary_3_youngCohort
small RNA

data_processing:
Adaptor sequences were trimmed from reads using cutadapt (Martin 2011). Reads were filtered for quality using fastq_quality_filter (FastX Toolkit; http://hannonlab.cshl.edu/fastx_toolkit/) with the parameters “-q 30 –p 100” to ensure that all bases had an accuracy of 99.9%. To assign processed sequencing reads to miRNAs, the first 19nt of each read were matched to a dictionary of miRNA sequences downloaded from TargetScanMouse Release 7, requiring no mismatches between the read and the miRNA dictionary. Reads mapping to the internal size standards and spike-in miRNAs were removed for further analysis. Differential expression analysis was performed using DESeq2 v1.38.3 without use of the lfcShrink() function.
Assembly: TargetScanMouse Release 7
Supplementary files format and content: tab-delimited text file of DESeq2 output includes miRNA name (mirna), baseMean, log2 fold change, lfcSE, stat, p-value, adjusted p-value, raw counts, and normalized counts

extract_protocol:
Total RNA was extracted from mouse tissues using TRI Reagent (ThermoFisher) according to the manufacturer’s protocol with the following modifications. Mouse tissues were rapidly dissected after CO2-based euthanasia and flash frozen in Eppendorf tubes in liquid N2. Tissue was transferred to a 15 mL conical tube, 1–2 mL of TRI Reagent was added, and the tissue was homogenized with a TissueRuptor (Qiagen) and disposable probes. Samples were transferred to Eppendorf tubes (1 mL per tube) and phase separated with 200 μL chloroform (J.T. Baker Analytical). After isopropanol precipitation and two 70% ethanol washes, total RNA was resuspended in water.
Each small-RNA sequencing libraries was prepared with 3 ug of total RNA extracted from mouse pituitary. In addition, 0.5 fmoles of miR-427-5p (X. tropicalis) and 0.5 fmoles of lsy-6-3p (C. elegans) were added as spike-ins. From each sample, RNAs that co-migrated within the range of 18 and 32 nt radiolabeled internal standards were isolated on a 15% polyacrylamide urea gel. Purified RNA was ligated to a preadenylated 3' adapter (AppNNNNCTGTCTCTTATACACATCTCCGAGCddC) using T4 RNA Ligase 2 KQ mutant (NEB) in a reaction supplemented with 10% polyethylene glycol (PEG 8000, NEB). To reduce ligation biases, this 3' adapter had 4 random-sequence positions at its 5' end. After gel purification on a 8% polyacrylamide urea gel, RNA was ligated to a 5' adapter (CCUACACGACGCUCUUCCGAUCUNNNN) using T4 RNA Ligase I (NEB) in a reaction supplemented with 10% PEG. To reduce ligation biases, this adapter had 4 random-sequence positions at its 3' end. After gel purification on an 8% polyacrylamide urea gel, RNA was reverse transcribed with SuperScript III (Invitrogen), and the cDNA was amplified ~12 cycles with KAPA HiFi DNA polymerase (Kapa Biosystems). Amplified DNA was purified using 1.8X AmpureXP (Beckman Coulter) bead cleanup, followed by agarose gel on the PippinHT. A step-by-step protocol for constructing libraries for small-RNA sequencing is available at http://bartellab.wi.mit.edu/protocols.html

value: 8–12 weeks old


title: miRNA-Seq, Zeb1_200M_pituitary_2_youngCohort

description:
Library name: Zeb1_200M_pituitary_2_youngCohort
small RNA


title: miRNA-Seq, Zeb1_200M_pituitary_4_youngCohort

description:
Library name: Zeb1_200M_pituitary_4_youngCohort
small RNA


title: miRNA-Seq, Zeb1_200H_pituitary_4_youngCohort

description:
Library name: Zeb1_200H_pituitary_4_youngCohort
small RNA


title: smallRNA-Seq Zeb1_200M_pituitary_3

description: small RNA


title: miRNA-Seq, Zeb1_200M_pituitary_1_youngCohort

description:
Library name: Zeb1_200M_pituitary_1_youngCohort
small RNA


title: smallRNA-Seq Zeb1_WT_pituitary_2

description: small RNA


title: smallRNA-Seq Zeb1_WT_pituitary_1

description: small RNA


title: miRNA-Seq, Zeb1_200H_pituitary_2_youngCohort

description:
Library name: Zeb1_200H_pituitary_2_youngCohort
small RNA


title: smallRNA-Seq Zeb1_200H_pituitary_4

description: small RNA


title: smallRNA-Seq Zeb1_200M_pituitary_2

description: small RNA


title: uninfected_1, run 3

city: Ann Arbor

first: Marc

middle: B

last: Hershenson

state: MI

address: 1150 W. Medical Center Drive

department: Department of Pediatric Pulmonology

institute: Michigan Medicine

description: Library name: 13215-JK-10

data_processing:
The reads were trimmed using Cutadapt v4.8 (Martin, 2011). The reads were evaluated with FastQC (Andrews, 2010) (v0.11.8) to determine quality of the data.
Reads were mapped to the reference genome GRCh38 (ENSEMBL 109), using STAR v2.7.8a (Dobin et al., 2013) and assigned count estimates to genes with RSEM v1.3.3 (Li and Dewey, 2011). Alignment options followed ENCODE standards for RNA-seq (Dobin).
QC metrics from several different steps in the pipeline were aggregated by multiQC v1.20 (Ewels et al., 2016).
Assembly: GRCh38 (ENSEMBL 109)
Supplementary files format and content: Tab-deliminated text file includes a complete matrix of gene expected count totals for all samples.

molecule: polyA RNA

source_name: nasal swab

extract_protocol:
Total RNA from nasal swab samples was prepared using Trizol extraction. RNA was purified using RNEasy spin columns and enriched for mRNA using a Poly(A) mRNA Magnetic Isolation Module.
A TruSeq-based Takara kit was used during library preparation. This pool was subjected to 151bp paired-end sequencing according to the manufacturer’s protocol (Illumina NovaSeqXPlus). BCL Convert Conversion Software v4.0 (Illumina) was used to generate de-multiplexed Fastq files.
Library prep and next-generation sequencing was carried out in the Advanced Genomics Core at the University of Michigan.

value: nasal swab

tag: host viral_infection

value: uninfected

tag: library batch


title: uninfected_10, run 2

description: Library name: 13215-JK-13


title: smallRNA-Seq Zeb1_200H_pituitary_1

description: small RNA


title: miRNA-Seq, Zeb1_200H_pituitary_3_youngCohort

description:
Library name: Zeb1_200H_pituitary_3_youngCohort
small RNA


title: uninfected_9, run 2

description: Library name: 13215-JK-12


title: miRNA-Seq, Zeb1_WT_pituitary_4_youngCohort

description:
Library name: Zeb1_WT_pituitary_4_youngCohort
small RNA


title: miRNA-Seq, Zeb1_WT_pituitary_1_youngCohort

description:
Library name: Zeb1_WT_pituitary_1_youngCohort
small RNA


title: uninfected_2, run 3

description: Library name: 13215-JK-11


title: uninfected_12, run 1

description: Library name: 13215-JK-8


title: miRNA-Seq, Zeb1_WT_pituitary_2_youngCohort

description:
Library name: Zeb1_WT_pituitary_2_youngCohort
small RNA


title: uninfected_13, run 1

description: Library name: 13215-JK-9


title: miRNA-Seq, Zeb1_200H_pituitary_1_youngCohort

description:
Library name: Zeb1_200H_pituitary_1_youngCohort
small RNA


title: uninfected_11, run 1

description: Library name: 13215-JK-7


title: RV (untyped)_4, run 1

description: Library name: 13215-JK-5

value: RV (untyped)


title: RV (untyped)_3, run 1

description: Library name: 13215-JK-4


title: uninfected_5, run 2

description: Library name: 11119-JK-42


title: RV (untyped)_1, run 1

description: Library name: 13215-JK-1


title: uninfected_4, run 2

description: Library name: 11119-JK-41


title: uninfected_10, run 1

description: Library name: 11119-JK-47


title: uninfected_9, run 1

description: Library name: 11119-JK-46


title: RSV_11, run 1

description: Library name: 11119-JK-39

value: RSV


title: uninfected_6, run 2

description: Library name: 11119-JK-43


title: RSV_10, run 1

description: Library name: 11119-JK-38


title: uninfected_7, run 1

description: Library name: 11119-JK-44


title: RSV_9, run 1

description: Library name: 11119-JK-37


title: RVC_30, run 1

description: Library name: 11119-JK-36

value: RV-C


title: RVA_16, run 1

description: Library name: 11119-JK-35

value: RV-A


title: RV (untyped)_5, run 1

description: Library name: 13215-JK-6


title: RVA_15, run 1

description: Library name: 11119-JK-30


title: RVC_29, run 1

description: Library name: 11119-JK-34


title: RVC_26, run 1

description: Library name: 11119-JK-29


title: RV (untyped)_2, run 1

description: Library name: 13215-JK-3


title: RVC_28, run 1

description: Library name: 11119-JK-32


title: RVA_14, run 1

description: Library name: 11119-JK-28


title: RVC_25, run 1

description: Library name: 11119-JK-27


title: RVA_13, run 1

description: Library name: 11119-JK-26


title: RSV_12, run 1

description: Library name: 11119-JK-40


title: RVC_27, run 1

description: Library name: 11119-JK-31


title: RVC_23, run 1

description: Library name: 11119-JK-24


title: RVC_24, run 1

description: Library name: 11119-JK-25


title: RVA_9, run 1

description: Library name: 11119-JK-15


title: RVC_20, run 1

description: Library name: 11119-JK-20


title: RVA_12, run 1

description: Library name: 11119-JK-21


title: RVC_21, run 1

description: Library name: 11119-JK-22


title: RVC_19, run 1

description: Library name: 11119-JK-14


title: RVA_10, run 1

description: Library name: 11119-JK-16


title: RVC_22, run 1

description: Library name: 11119-JK-23


title: RVC_17, run 1

description: Library name: 11119-JK-12


title: RVC_11, run 1

description: Library name: 11119-JK-4


title: RVC_12, run 1

description: Library name: 11119-JK-5


title: uninfected_3, run 2

description: Library name: 10455-JK-11


title: RVA_11, run 1

description: Library name: 11119-JK-19


title: RVC_9, run 1

description: Library name: 11119-JK-2


title: RVC_16, run 1

description: Library name: 11119-JK-10


title: uninfected_8, run 1

description: Library name: 11119-JK-45


title: RVC_13, run 1

description: Library name: 11119-JK-6


title: RVA_8, run 1

description: Library name: 11119-JK-8


title: RVC_14, run 1

description: Library name: 11119-JK-7


title: RSV_7, run 1

description: Library name: 10455-JK-7


title: RVC_18, run 1

description: Library name: 11119-JK-13


title: RVC_10, run 1

description: Library name: 11119-JK-3


title: RVC_8, run 1

description: Library name: 11119-JK-1


title: uninfected_1, run 2

description: Library name: 10455-JK-9


title: RSV_8, run 1

description: Library name: 10455-JK-8


title: uninfected_2, run 2

description: Library name: 10455-JK-10


title: RVC_15, run 1

description: Library name: 11119-JK-9


title: RSV_6, run 1

description: Library name: 10455-JK-6


title: RSV_3, run 1

description: Library name: 10455-JK-3


title: RSV_2, run 1

description: Library name: 10455-JK-2


title: uninfected_4, run 1

description: Library name: 9400-JK-19


title: uninfected_1, run 1

description: Library name: 9400-JK-16


title: uninfected_3, run 1

description: Library name: 9400-JK-18


title: RVC_6, run 1

description: Library name: 9400-JK-14


title: RVC_4, run 1

description: Library name: 9400-JK-12


title: RSV_5, run 1

description: Library name: 10455-JK-5


title: RVC_3, run 1

description: Library name: 9400-JK-11


title: RVC_2, run 1

description: Library name: 9400-JK-7


title: RSV_1, run 1

description: Library name: 10455-JK-1


title: RSV_4, run 1

description: Library name: 10455-JK-4


title: uninfected_5, run 1

description: Library name: 9400-JK-20


title: RVA_3, run 1

description: Library name: 9400-JK-3


title: RVA_1, run 1

description: Library name: 9400-JK-1


title: uninfected_2, run 1

description: Library name: 9400-JK-17


title: RVC_7, run 1

description: Library name: 9400-JK-15


title: RVC_5, run 1

description: Library name: 9400-JK-13


title: RVA_7, run 1

description: Library name: 9400-JK-10


title: RVC_1, run 1

description: Library name: 9400-JK-6


title: RVA_6, run 1

description: Library name: 9400-JK-9


title: 584T3M_IDH2-WT_SRSF2-Mutant BS

status: Public on Nov 26 2025

city: Miami

first: Aristeidis

middle: G

last: Telonis

state: FL

address: 1550 NW 10th Ave

institute: University of Miami

data_processing:
Adapters were trimmed from the acquired fastq files with cutadapt (v. 1.18) with parameter -m25 -U10
Reads were mapped on the GRCh37 human genome with bismark and bismark_methylation_extractor (v. 0.22.1)
Assembly: GRCh37
Supplementary files format and content: Genome-wide cytosine report file (chromosome, position, strand, count methylated, count unmethylated, C context, trinucleotide context)

source_name: Marrow

extract_protocol:
AllPrep DNA/RNA kit from Qiagen (Valencia, CA)
Methyl-Seq Kit (Swift Biosciences)

value: Marrow

value: F

tag: idh2 genotype

value: WT

tag: srsf2 genotype

value: Mutant


title: 736T3B_IDH2-WT_SRSF2-Mutant BS

source_name: Blood

value: Blood


title: 495T1M_IDH2-Mutant_SRSF2-Mutant BS


title: 560T3B_IDH2-WT_SRSF2-Mutant BS

value: M


title: 614T1M_IDH2-Mutant_SRSF2-Mutant BS


title: 364T2M_IDH2-WT_SRSF2-WT BS


title: 316T1B_IDH2-Mutant_SRSF2-Mutant BS


title: 475T1B_IDH2-WT_SRSF2-Mutant BS


title: 556T2B_IDH2-Mutant_SRSF2-Mutant BS


title: 287T1M_IDH2-WT_SRSF2-Mutant BS


title: 54T1B_IDH2-WT_SRSF2-WT BS


title: 543T6B_IDH2-Mutant_SRSF2-WT BS


title: RVA_5, run 1

description: Library name: 9400-JK-8


title: 523T2B_IDH2-Mutant_SRSF2-Mutant BS


title: RVA_2, run 1

description: Library name: 9400-JK-2


title: 603T2M_IDH2-WT_SRSF2-Mutant BS


title: 633T1M_IDH2-WT_SRSF2-Mutant BS


title: 246T9B_IDH2-Mutant_SRSF2-WT BS


title: 283T9B_IDH2-Mutant_SRSF2-Mutant BS


title: 587T2B_IDH2-WT_SRSF2-WT BS


title: 225T15B_IDH2-WT_SRSF2-Mutant BS


title: 247T2Bb_IDH2-Mutant_SRSF2-Mutant BS


title: 614T1M_IDH2-Mutant_SRSF2-Mutant RNA

data_processing:
Adapters were trimmed from the acquired fastq files with cutadapt (v. 1.18) with parameter -m10
Reads were mapped on the GRCh37 human genome with STAR (v. 2.7.1a) with parameters chimSegmentMin=10, outFilterMismatchNmax=5, alignEndsType=EndToEnd, alignIntronMax=1000000 and alignSJDBoverhangMin=6
Gene expression profiles were extracted with RSEM (v. 1.2.28) using Ensembl v75 for gene annotations
Assembly: GRCh37
Supplementary files format and content: Table with counts and FPKM values per gene

extract_protocol:
AllPrep DNA/RNA kit from Qiagen (Valencia, CA)
polyA-selected strand-specific SureSelect (Agilent)


title: 247T2Ba_IDH2-Mutant_SRSF2-Mutant BS


title: 560T3B_IDH2-WT_SRSF2-Mutant RNA


title: 234T1B_IDH2-WT_SRSF2-WT BS


title: 201T1B_IDH2-Mutant_SRSF2-WT BS


title: 556T2B_IDH2-Mutant_SRSF2-Mutant RNA


title: 54T1B_IDH2-WT_SRSF2-WT RNA


title: 648T1B_IDH2-Mutant_SRSF2-WT RNA


title: 247T2Bb_IDH2-Mutant_SRSF2-Mutant RNA


title: 495T1M_IDH2-Mutant_SRSF2-Mutant RNA


title: 523T2B_IDH2-Mutant_SRSF2-Mutant RNA


title: 85T1M_IDH2-WT_SRSF2-WT RNA


title: 736T3b_IDH2-WT_SRSF2-Mutant RNA


title: 283T9B_IDH2-Mutant_SRSF2-Mutant RNA


title: 287T1M_IDH2-WT_SRSF2-Mutant RNA


title: 246T9B_IDH2-Mutant_SRSF2-WT RNA


title: 543T6B_IDH2-Mutant_SRSF2-WT RNA


title: 225T15B_IDH2-WT_SRSF2-Mutant RNA


title: 584T3M_IDH2-WT_SRSF2-Mutant RNA


title: 475T1B_IDH2-WT_SRSF2-Mutant RNA


title: 587T2B_IDH2-WT_SRSF2-WT RNA


title: 247T2Ba_IDH2-Mutant_SRSF2-Mutant RNA


title: E59 human distal femur ATAC

city: Boston

first: April

middle: M

last: Craft

address: 300 Longwood Ave

institute: Boston Children's Hospital

library_source: genomic single cell

data_processing:
the Cell Ranger pipeline was used to process the fastq files
the Cell Ranger-ARC pipeline v2.0.0 was used to generate the count matrix
Assembly: hg38
Supplementary files format and content: Cell ranger output files - barcodes.tsv, features.tsv, matrix.mtx, atac_fragments.tsv

source_name: distal femur

extract_protocol:
collagenase digestion
RNA and ATAC seq libraries were prepared using the 10x Chromium Single Cell Multiome ATAC + Gene Expression kit following the manufacturer's protocols

value: distal femur

value: Collagenase digestion


title: 201T1B_IDH2-Mutant_SRSF2-WT RNA


title: M3

status: Public on Nov 25 2025

city: Charleston

first: Stefano

last: Berto

state: SC

address: 68 President Street, BE208

institute: MUSC

description: Library name: Sample 27

data_processing:
RNA-seq reads were aligned to the human reference genome (hg38) using STAR (v2.7.1a). For each sample, a BAM file was generated containing both mapped and unmapped reads across splice junctions. Secondary alignments and multi-mapped reads were removed using in-house scripts, and only uniquely mapped reads were retained for downstream analyses. GENCODE annotation for hg38 (version 32) was used for reference-guided alignment and downstream quantification. Gene-level expression was quantified using featureCounts (v2.0.1) , based on protein-coding genes from the annotation files.
Assembly: Hg38
Supplementary files format and content: gene_count

source_name: renal mesangial

extract_protocol:
hRMCs (lots 12445, 17544, and 20018) were treated in quadruplicate wells in 6-well plates withvehicle, 25 ng/ml IL-1b, or 25 ng/ml IL-1b +20 nM eliglustat for 24, 48, or 72 hrs. Media was collected for ELISAs and GSL quantification, cell viability was measured by the alamar blue assay, and cells scraped into DPBS and replicate wells combined. The cell suspension was split into two tubes, centrifuged, and DPBS removed. Half the cells were used for GSL quantification, and the other half were used for RNA isolation.
RNA was isolated using RNeasy kit with QiaShredders, resuspended in RNase free water and the concentration and quality assessed by Nanodrop. A total of 2 μg RNA per sample was shipped to Novagene for library preparation and sequencing after passing their quality control (QC) checks.

value: renal mesangial


title: E59 human distal femur RNA


title: M1

description: Library name: Sample 25


title: E72 human distal femur RNA


title: E67_knee_spatial01

description:
Library name: D1_k67_spatialHD
10X Genomics Visium HD

data_processing:
The raw files were mapped to the Human genome Hg38 using the Space Ranger v.4.0 software, available via the 10X Genomics website.
Assembly: hg38
Supplementary files format and content: space ranger output files: fragments, matrix and barcodes are provided.
Supplementary files format and content: Loupe file.
Library strategy: Spatial Transcriptomics

source_name: knee

extract_protocol:
Fresh knee tissues were dissected, flash frozen in OCT, sectioned at 10um, permeabalized, and follwed the 10X Genomics spatial Visium HD manufactureres protocol. cDNA was made, amplified and the DNA was purified using Ampure beads.
Libraries were constructed following 10X Genomics visium manufactures protocols. Quality was checked using the tapestation, libraries were pooled and eventually sequenced using the NovaSeq X.

value: knee


title: M2

description: Library name: Sample 26


title: K3

description: Library name: Sample 21


title: J3

description: Library name: Sample 18


title: L3

description: Library name: Sample 24


title: E72 human distal femur ATAC


title: L2

description: Library name: Sample 23


title: I2

description: Library name: Sample 14


title: L1

description: Library name: Sample 22


title: I1

description: Library name: Sample 13


title: H3

description: Library name: Sample 12


title: K2

description: Library name: Sample 20


title: K1

description: Library name: Sample 19


title: J2

description: Library name: Sample 17


title: I3

description: Library name: Sample 15


title: J1

description: Library name: Sample 16


title: H2

description: Library name: Sample 11


title: E1

description: Library name: Sample 1


title: G2

description: Library name: Sample 8


title: H1

description: Library name: Sample 10


title: KO 1

status: Public on Nov 18 2025

city: New York

first: Ankit

last: Gilani

state: New York

address: 413E 69th Street

institute: Weill Cornell Medical College

description: Library name: Sample 6

data_processing:
CLC Genomics workbench v 11.0.1
Assembly: mm10
Supplementary files format and content: tab delimited text file includes raw counts for each samples

source_name: Inguinal SVF

extract_protocol:
RNA was harvested using Rneasy mini kit (Qiagen). 1 ug of total RNA was used for the construction of sequencing libraries
RNA libraries for RNA-Seq were prepared using SMARTER mRNA-Seq library prep kit using manufacturer's protocols.

value: Inguinal SVF

tag: cell line

value: Primary Adipocytes

value: Adipogenic differentiation'


title: F2

description: Library name: Sample 5


title: F1

description: Library name: Sample 4


title: G1

description: Library name: Sample 7


title: KO 3

description: Library name: Sample 8


title: F3

description: Library name: Sample 6


title: E3

description: Library name: Sample 3


title: E2

description: Library name: Sample 2


title: KO 5

description: Library name: Sample 10


title: Control 1

description: Library name: Sample 1


title: G3

description: Library name: Sample 9


title: KO 4

description: Library name: Sample 9


title: Control 5

description: Library name: Sample 5


title: KO 2

description: Library name: Sample 7


title: Control 3

description: Library name: Sample 3


title: Control 4

description: Library name: Sample 4


title: Inoculated, 44 dpi, rep4

status: Public on Nov 08 2025

city: Hatfield

first: Dave

middle: Kenneth

last: Berger

state: Gauteng

address: Plant Sciences Complex, Entrance 11 - Corner Lunnon Road & Herold Street

department: Dept. Plant and Soil Sciences, Forestry and Agricultural Biotechnology Institute (FABI)

country: South Africa

institute: University of Pretoria

description: Library name: Zm_Cz_44dpi_rep4

data_processing:
Galaxy V24.1
Raw RNA-seq reads were assed using FastQC to evaluate the quality of data. Trimmomatic was used to process the data to remove adapter sequences, low quality reads (AVGQUAL > 20) and short reads (MINLEN  > 35).  High quality reads were then mapped to the concatenated maize-C. zeina genome using HiSAT2 with default settings. The number of reads mapped to each gene was then computed by htseq-count using the union mode with a minimum alignment quality of 10. Data normalisation and differential expression analyses were computed using DEseq2 with a single factor (group) and three factor levels (early (23 dpi), mid (30dpi) and late (44 dpi)). Pre-filtering (N > 38) was used to remove low expressed genes from the dataset.
Assembly: GCA_002844615.2 (Cercospora zeina) and GCA_902167145.1 (Maize)
Supplementary files format and content: Tab-delimited text file with normalized count values for each sample

organism:
Zea mays
Cercospora zeina

source_name: Leaf

extract_protocol:
Total RNA was isolated from each sample using the QIAzol lysis reagent (Qiagen, Hilden, USA) followed by purification using the NucleoSpin RNA purification kit (Machery-Nagel, Duren, Germany). 1 µg of each sample was used for library preparation
library preparation was done using a P3 PE100 kit (Illumina Inc. San Diego, USA) following manufacturer instructions

value: Leaf

value: Inoculated, 44 dpi


title: Control 2

description: Library name: Sample 2


title: Inoculated, 44 dpi, rep2

description: Library name: Zm_Cz_44dpi_rep2


title: Inoculated, 44 dpi, rep3

description: Library name: Zm_Cz_44dpi_rep3


title: Inoculated, 23 dpi, rep4

description: Library name: Zm_Cz_23dpi_rep4

value: Inoculated, 23 dpi


title: Inoculated, 30 dpi, rep1

description: Library name: Zm_Cz_30dpi_rep1

value: Inoculated, 30 dpi


title: Inoculated, 44 dpi, rep1

description: Library name: Zm_Cz_44dpi_rep1


title: MCF10A, 2.5 µM etoposide

status: Public on Oct 30 2025

city: Boulder

first: Brianna

last: Fernandez

state: CO

address: 3415 Colorado Avenue

department: Biochemistry

institute: University of Colorado Boulder

description:
Library name: Sample 2
10x Genomics

data_processing:
scRNA-seq data were processed and analyzed using the Seurat (v5.1.0) package in R (v4.4.1). Raw sequencing reads were pre-processed using CellRanger (v7.1.0) and aligned to the Human (GRCh38) 2020-A reference genome. Gene-cell count matrices were generated, and low-quality cells were filtered out based on the number of detected genes, unique molecular identifiers (UMIs), and mitochondrial gene percentage. Cells with fewer than 3,500 genes were excluded to remove low-quality cells. Cells with greater than 15% mitochondrial gene expression were also removed. Data were log-normalized using Seurat’s NormalizeData() function with a scale factor of 10,000.
Assembly: Human (GRCh38) 2020-A
Supplementary files format and content: matrix files for counts, features, and barcodes.
Supplementary files format and content: merged_filtered_etop.h5ad: This file contains a .h5ad format file of the merged and filtered seurat object with all treatments combined and metadata annotated (i.e., sample, dose, cluster ID, UMAP dimensions, etc.)
Supplementary files format and content: merged_filtered_etop.rds: This file contains a .rds format file of the merged and filtered seurat object with all treatments combined and metadata annotated (i.e., sample, dose, cluster ID, UMAP dimensions, etc.)

extract_protocol:
Single-cell suspensions were prepared following the 10x Genomics “Cell Preparation for Single Cell Protocols” guide with a viability of >95%. Single-cell encapsulation, barcoding, cDNA amplification, library preparation, and sequencing were performed by the University of Colorado Anschutz Genomics Shared Resource facility. Cells were encapsulated into droplets using the Chromium Controller, where reverse transcription occurred within individual gel beads-in-emulsion (GEMs).
Single-cell RNA sequencing (scRNA-seq) libraries were generated using the 10x Genomics Chromium platform with the 3' mRNA capture kit. Single-cell suspensions were prepared following the 10x Genomics “Cell Preparation for Single Cell Protocols” guide with a viability of >95%. Single-cell encapsulation, barcoding, cDNA amplification, library preparation, and sequencing were performed by the University of Colorado Anschutz Genomics Shared Resource facility. Cells were encapsulated into droplets using the Chromium Controller, where reverse transcription occurred within individual gel beads-in-emulsion (GEMs). Following barcoding and cDNA amplification, sequencing libraries were constructed according to the manufacturer’s protocol. Libraries were sequenced on an Illumina NovaSeqX to a depth of 81,810 reads per cell for untreated, 74,214 reads per cell for 2.5µM, 81,254 reads per cell for 10µM, and 76,973 reads per cell for 25µM.

treatment_protocol: MCF10A cells were split 1:10 (from a confluent plate) into a plastic 10cm culture plate before being treated with 2.5, 10, or 25μM etoposide the following day for 24 h. Cells were then washed twice with PBS before being returned to full-growth media. Full-growth media was refreshed every 3d during the drug recovery period.

value: normal human breast epithelial cell

value: 2.5 µM etoposide


title: Inoculated, 23 dpi, rep1

description: Library name: Zm_Cz_23dpi_rep1


title: VDJ seq - lane B

status: Public on Oct 06 2025

city: Parkville

first: Jan

last: Schroeder

state: Victoria

address: Peter Doherty Institute Melbourne

department: DMI

country: Australia

institute: University of Melbourne

description: TCR-B

data_processing:
Single cell reads for RNA and CITE-seq antibodies are mapped to the human transcriptome and counted using cellranger (version 6.1.2)
TCR-seq reads are mapped and assembled using cellranger
Assembly: hg38
Supplementary files format and content: h5 files forGEX/CITE assays
Supplementary files format and content: csv of mapped and assembled VDJ reads for TCR assay
Supplementary files format and content: csv formatted metadata for filtered and annotated single cells

source_name: breast/blood

growth_protocol: ex vivo

extract_protocol:
CD3 positive cells were sorted from fresh breast tissue sections
Sample preparation followed by 10x Genomics scRNA-seq v3 chemistry protocol. Samples were processed as described on the Chromium Single Cell 3' Reagent Kits User Guide (v3 Chemistry).

value: breast/blood

value: CD3 pos cells

value: WT


title: Inoculated, 30 dpi, rep2

description: Library name: Zm_Cz_30dpi_rep2


title: Inoculated, 23 dpi, rep2

description: Library name: Zm_Cz_23dpi_rep2


title: Inoculated, 30 dpi, rep3

description: Library name: Zm_Cz_30dpi_rep3


title: MCF10A, 10 µM etoposide

description:
Library name: Sample 3
10x Genomics

value: 10 µM etoposide


title: Inoculated, 23 dpi, rep3

description: Library name: Zm_Cz_23dpi_rep3


title: VDJ seq - lane C

description: TCR-C


title: VDJ seq - lane A

description: TCR-A


title: CITE seq - lane C

description: CITE-C

molecule: protein


title: CITE seq - lane A

description: CITE-A


title: MCF10A, 25 µM etoposide

description:
Library name: Sample 4
10x Genomics

value: 25 µM etoposide


title: Inoculated, 30 dpi, rep4

description: Library name: Zm_Cz_30dpi_rep4


title: CITE seq - lane B

description: CITE-B


title: RNA seq - lane B

description: GEX-B


title: RNA seq - lane C

description: GEX-C