title: Coculture WT Control 2

status: Public on Feb 16 2026

first: Yajing

last: Liu

state: NY

address: 455 Main Street, Apt 6V

institute: Weill cornell medicine

description: Library name: Sample 17

data_processing:
Raw data (raw reads) of fastq format were firstly processed throughfastpsoftware. In this step, clean data (clean reads) were obtained by removing reads containing adapter, reads containing ploy-Nandlowquality reads from raw data.
At the same time, Q20, Q30 and GCcontent the clean data were calculated. All the downstreamanalyses were basedon the clean data with high quality.
Gene-level counts were quantified using featureCounts, and differential gene expression analysis was performed in R using DESeq2 package.
Assembly: hg38
Supplementary files format and content: tab-delimited text file includes raw counts for each Sample

source_name: human iPSC

growth_protocol: iPSC lines were maintained in mTeSR™ Plus medium on Matrigel-coated plates at 37°C with 5% CO₂

extract_protocol:
Total RNA was isolated from cells using RNeasy Mini Kit (Qiagen) according to manufacturer protocols.
Library preparation and bulk RNA sequencing were performed by Novogene by sequencing on NovaSeq platform.

treatment_protocol: cGAS inhibitor was added to a final concentration of 10 µM (with DMSO as control)

value: human iPSC

value: human iPSC derived microglia motor neuron co culture

value: WT Control


title: Coculture WT Control 3

type: SRA

city: New York

country: USA

description: Library name: Sample 18

library_source: transcriptomic

molecule: total RNA

organism: Homo sapiens

tag: cell line

tag: cell type

tag: genotype

value: WT

tag: treatment


title: Coculture WT Control 1

description: Library name: Sample 16


title: Coculture Q331K hcGASi 2

description: Library name: Sample 14

value: Q331K hcGASi


title: Coculture Q331K hcGASi 3

description: Library name: Sample 15


title: WT mice,neutrophil+LPS,rep1

status: Public on Feb 17 2026

city: Shanghai

first: Xiaoqian

last: Hu

address: No. 130, Dongan Road, Xuhui District, Shanghai

country: China

institute: Fudan University

description: Library name: WT_LPS_1A

data_processing:
1. Readscontainingadapters(adaptercontamination); 2. Readswithanunknownbase'N'contentexceeding0.1%;3. Low-qualityreads(readswithaproportionofbaseswithaqualityvaluebelow15exceeding20% of the total bases in the read), resulting in clean data.
Assembly: Mus_musculus_10090.NCBI.GCF_000001635.26_GRCm38.p6.v2201
Supplementary files format and content: The table contains information about the RNA modification sites identified for each sample as well as RNA counts and standardized values

organism: Mus musculus

source_name: neutrophil

extract_protocol:
1. Sample Lysis For cultured cells:  Remove the culture medium and wash the cells with cold PBS. Add 1 mL of TRIzol reagent per 1 × 10⁶ cells. Pipette up and down to lyse the cells completely. For tissue samples:  Quickly freeze the tissue in liquid nitrogen and grind it into powder using a mortar and pestle. Transfer the tissue powder into a tube containing 1 mL of TRIzol reagent. Homogenize the sample thoroughly until no visible tissue fragments remain. 2. Phase Separation Incubate the lysate at room temperature for 5 minutes. Add 200 µL of chloroform per 1 mL of TRIzol reagent. Vortex vigorously for 15 seconds and incubate at room temperature for 3 minutes. Centrifuge at 12,000 × g for 15 minutes at 4°C. After centrifugation, three layers should appear: Upper aqueous phase (RNA) Middle interphase (proteins) Lower organic phase (DNA and lipids) Carefully transfer the upper aqueous phase to a new RNase-free tube without disturbing the interphase. 3. RNA Precipitation Add an equal volume of isopropanol to the transferred aqueous phase. Mix gently by inverting the tube several times. Incubate at -20°C for at least 10 minutes (or overnight for better yield). Centrifuge at 12,000 × g for 10 minutes at 4°C. Discard the supernatant, leaving the RNA pellet at the bottom. 4. RNA Washing Add 1 mL of 75% ethanol to wash the pellet. Vortex briefly and centrifuge at 7,500 × g for 5 minutes at 4°C. Remove the supernatant carefully and air-dry the pellet for ~5 minutes (avoid over-drying). 5. RNA Resuspension Dissolve the RNA pellet in 20–50 µL of RNase-free water. Incubate at 55°C for 5–10 minutes to fully dissolve the RNA. Store the RNA at -80°C for long-term use.
For library construction, total RNA was processed using mRNA enrichment. The mRNA was enriched using magnetic beads with Oligo(dT) to capture mRNA with a polyA tail. The obtained RNA was fragmented using a breaking buffer, and random N6 primers were used for reverse transcription. Subsequently, double-stranded cDNA was synthesized. The ends of the synthesized double-stranded DNA were blunted and 5' end-phosphorylated. A single-base overhang "A" was added to the 3' end, and a bubble-shaped adapter with a protruding "T" at the 3' end was ligated. The ligated products were PCR-amplified using specific primers. The PCR products were denatured to form single-stranded DNA, and single-stranded circular DNA libraries were generated using a bridged primer. The constructed libraries underwent quality control, and after passing, they were subjected to sequencing.

value: neutrophil

value: LPS


title: Holstein Friesian whole blood under heat stress, biological replicate 1

city: Zuru

first: Akeem

middle: Babatunde

last: Sikiru

state: Kebbi

address: Department of Animal Science, College of Agriculture, FUAZ, Zuru, Nigeria.

department: Animal Science

country: Nigeria

institute: Federal University of Agriculture Zuru

description:
Library name: Holstien-Friesian1_L1_1.fq.gz
Female, non-pregnant, non-lactating, age 4.5±1.2 years, body weight 450±15 kg. Sample collected from jugular vein during peak heat load.

data_processing:
Libraries constructed using Illumina Stranded mRNA Prep kit. mRNA enriched by poly-A selection, fragmented, reverse-transcribed. Libraries end-repaired, A-tailed, ligated with indexed adapters, amplified by PCR. Quality assessed using Agilent 2100 Bioanalyzer. Sequenced on Illumina NovaSeq 6000 (paired-end, 2 × 150 bp).
Raw reads quality assessed with FastQC v0.11.9. Adapter trimming and quality filtering with Trimmomatic v0.39.
Reads aligned to Bos taurus reference genome (ARS-UCD1.2) using HISAT2 v2.2.1.
Gene counts quantified using featureCounts v2.0.1. Expression normalized as FPKM.
Assembly: Differential expression analysis using DESeq2 v1.38.0. Genes with adjusted p < 0.05 and log2FC > 1 considered differentially expressed.
Supplementary files format and content: ARS-UCD1.2
Supplementary files format and content: raw_counts.txt: Tab-delimited gene count matrix. FPKM_normalized_expression.txt: FPKM-normalized values. differential_expression_results.txt: DESeq2 results with gene symbols, log2FC, p-values, adjusted p-values.
Supplementary files format and content: tab-delimited text file includes raw counts for each Sample

organism: Bos taurus

source_name: whole blood

growth_protocol: Clinically healthy, non-pregnant, non-lactating adult female cows (mean age: 4.50 ± 1.20 years; mean body weight: 450.00 ± 15.00 kg) were maintained at Universiti Putra Malaysia Teaching and Research Farm, Serdang, Selangor, Malaysia (2°59'24"N, 101°42'32"E, elevation 68 m). The farm is characterized by a humid tropical climate with minimal seasonal temperature variation, mean annual temperature of 27.5°C, and relative humidity of 70-90%.

extract_protocol:
Animals were sampled during peak heat load conditions with Temperature-Humidity Index (THI) of 44.68. All experimental procedures were approved by IACUC (UPM/IACUC/AUP-R055/2025) and conducted in accordance with Malaysian Codes of Practice for Care and Use of Animals for Scientific Purposes and ARRIVE guidelines.
Total RNA was extracted from whole blood using PrimeZOL reagent (Canvax Biotech, Spain). RNA was precipitated with isopropanol, washed with 75% ethanol, and resuspended in nuclease-free water. RNA concentration and purity assessed by NanoDrop. RNA integrity evaluated with RIN > 7.0 required.

treatment_protocol: Heat stress (Temperature-Humidity Index: 44.68)

tag: tissue

value: whole blood

value: Holstein Friesian (heat-vulnerable temperate breed)

value: heat stress (Temperature-Humidity Index:44.68)


title: Coculture Q331K Control 3

description: Library name: Sample 12

value: Q331K Control


title: Coculture Q331K Control 1

description: Library name: Sample 10


title: iMGL HTDNA hcGASi 3

description: Library name: Sample 9

value: human iPSC derived microglia

value: HTDNA hcGASi


title: inguinal WAT, PT_HS_6

type: RNA

city: Ulm

first: Pamela

last: Fischer-Posovszky

address: Eythstr. 24, Haus 16, Forschungslabor KiKli

department: Department of Pediatrics and Adolescent Medicine

country: Germany

institute: Ulm University Medical Center

description: miRNA expression data from inguinal white adipose tissue of mice undergoing PT+HS

hyb_protocol: miRNAs were hybridized to AffymetrixTM miRNA 4.0 arrays

scan_protocol: Arrays were stained and washed on a GeneChip Fluidics Station 450 (Affymetrix)

data_processing: The arrays were analyzed by the Affymetrix GeneChip Scanner 3000 and the Affymetrix Expression ConsoleTM software. As only mouse miRNAs were used, the raw feature data were normalized using the RMA + DBAG algorithm and log2 intensity expression. A miRNA-transcriptome analysis was performed using BRB-ArrayTools developed by Dr. Richard Simon and BRB-ArrayTools Development Team. Only murine miRNAs were considered for further analysis.

label: biotin

source_name: Male C57BL/6 with PT+HS

label_protocol: 200 ng of total RNA were labelled using the FlashTagTM Biotin HSR RNA Labeling Kit (Genisphere, Hatfield, PA, USA).

growth_protocol: Male C57BL/6 mice aged 10-12 weeks were randomly assigned to sham treatment or PT + HS (n=5 animals per group)

extract_protocol: Whole tissues were minced in liquid nitrogen and afterwards resolved in Tri-Reagent. Total RNA was isolated with Direct-zol RNA mini Prep Kit (Zymo Research) according to the manufacturer’s protocol.

treatment_protocol: Mice were anesthetized with 2.5% sevoflurane in oxygen throughout the experiment before they were subjected to thoracic trauma, closed head injury, and femur fracture including soft tissue injury. Pressure-controlled hemorrhage was induced and monitored by a microcatheter in the femoral artery. A mean arterial pressure of around 30 ± 5 mmHg was maintained for 60 minutes. After resuscitation of the animals with a balanced electrolyte solution, inguinal WAT depots were removed 4 hours after trauma.

value: inguinal white adipose tissue


title: Holstein Friesian whole blood under heat stress, biological replicate 2

description:
Library name: Holstien-Friesian2_L1_1.fq.gz
Female, non-pregnant, non-lactating, age 4.5±1.2 years, body weight 450±15 kg. Sample collected from jugular vein during peak heat load.


title: iMGL HTDNA hcGASi 2

description: Library name: Sample 8


title: Coculture Q331K hcGASi 1

description: Library name: Sample 13


title: Coculture Q331K Control 2

description: Library name: Sample 11


title: iMGL HTDNA Control 3

description: Library name: Sample 6

value: HTDNA Control


title: iMGL HTDNA hcGASi 1

description: Library name: Sample 7


title: iMGL HTDNA Control 1

description: Library name: Sample 4


title: iMGL Control 1

description: Library name: Sample 1

value: Control


title: iMGL Control 3

description: Library name: Sample 3


title: iMGL Control 2

description: Library name: Sample 2


title: iMGL HTDNA Control 2

description: Library name: Sample 5


title: Untreated mt-DsRed2 hiNSCs 3D Culture, Control 6

city: Cincinnati

first: Volha

last: Liaudanskaya

state: Ohio

address: 3159 Eden Ave.

department: Biomedical Engineering

institute: University of Cincinnati

description: Library name: Nuntr-s6

data_processing:
Raw FASTQ reads were aligned to the Human GRCh38 (hg38) reference genome using STAR (v2.5.2b), and gene-level counts were generated via featureCounts. Differential expression was performed using DESeq2 in R, with raw counts normalized by the median-of-ratios method and p-values adjusted via the Benjamini-Hochberg procedure. Ensemble IDs were converted to HGNC symbols using biomaRt to facilitate comparison between in vitro results and human ASD datasets (GSE64018).
Assembly: GRCh38
Supplementary files format and content: The processed data file is a tab-delimited text file containing raw (un-normalized) read counts generated by featureCounts. The file includes columns for Geneid (Ensembl ID), genomic coordinates (Chr, Start, End, Strand), transcript Length, and the total number of aligned reads overlapping each gene for sample [Sample ID, e.g., M-s1].

source_name: HiNSC

extract_protocol:
RNA was harvested using RNeasy Kit (Qiagen, Catalog No. 74106).
RNA samples were quantified and checked for integrity. Sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina following the manufacturer's instructions. mRNAs were enriched, fragmented, and synthesized into first and second-strand cDNA. Adapters were ligated and libraries were enriched by PCR.

value: HiNSC

value: Induced Human Neural Stem Cells

value: Neuronal mitochondria tagged w/ DsRed2


title: Untreated mt-DsRed2 hiNSCs 3D Culture, Control 3

description: Library name: Nuntr-s3


title: Untreated mt-DsRed2 hiNSCs 3D Culture, Control 1

description: Library name: Nuntr-s1


title: mt-DsRed2 hiNSCs 3D Culture treated w/ mt isolated from mt-EBFP2 HMC3 3D Culture, Control 2

description: Library name: NTr-s2

value: Mitochondria isolated from mt-EBFP2 HMC3 3D Culture


title: mt-DsRed2 hiNSCs 3D Culture treated w/ mt isolated from mt-EBFP2 HMC3 3D Culture, Control 5

description: Library name: NTr-s5


title: mt-DsRed2 hiNSCs 3D Culture treated w/ mt isolated from mt-EBFP2 HMC3 3D Culture, Control 3

description: Library name: NTr-s3


title: mt-DsRed2 hiNSCs - mt-EBFP2 HMC3 3D Culture, Control 6

description: Library name: NM-s6

source_name: HiNSC - HMC3

value: HiNSC - HMC3

value: Induced Human Neural Stem Cells-Immortalized human microglial cells

value: Neuronal mitochondria tagged w/ DsRed2; Microglial mitochondria tagged w/ EBFP2


title: Untreated mt-DsRed2 hiNSCs 3D Culture, Control 5

description: Library name: Nuntr-s5


title: mt-DsRed2 hiNSCs - mt-EBFP2 HMC3 3D Culture, Control 4

description: Library name: NM-s4


title: mt-DsRed2 hiNSCs - mt-EBFP2 HMC3 3D Culture, Control 5

description: Library name: NM-s5


title: mt-DsRed2 hiNSCs 3D Culture treated w/ mt isolated from mt-EBFP2 HMC3 3D Culture, Control 4

description: Library name: NTr-s4


title: Untreated mt-DsRed2 hiNSCs 3D Culture, Control 2

description: Library name: Nuntr-s2


title: mt-EBFP2 HMC3 3D Culture, Control 5

description: Library name: M-s5

value: immortalized human microglial cells

value: Mitochondria tagged w/ EBFP2


title: mt-DsRed2 hiNSCs - mt-EBFP2 HMC3 3D Culture, Control 3

description: Library name: NM-s3


title: mt-DsRed2 hiNSCs - mt-EBFP2 HMC3 3D Culture, Control 2

description: Library name: NM-s2


title: mt-DsRed2 hiNSCs 3D Culture treated w/ mt isolated from mt-EBFP2 HMC3 3D Culture, Control 6

description: Library name: NTr-s6


title: mt-EBFP2 HMC3 3D Culture, Control 4

description: Library name: M-s4


title: mt-EBFP2 HMC3 3D Culture, Control 3

description: Library name: M-s3


title: mt-EBFP2 HMC3 3D Culture, Control 6

description: Library name: M-s6


title: mt-DsRed2 hiNSCs - mt-EBFP2 HMC3 3D Culture, Control 1

description: Library name: NM-s1


title: mt-EBFP2 HMC3 3D Culture, Control 2

description: Library name: M-s2


title: mt-DsRed2 hiNSCs 3D Culture treated w/ mt isolated from mt-EBFP2 HMC3 3D Culture, Control 1

description: Library name: NTr-s1


title: K21(TI3)(Small)

city: torrance

first: Omid

last: Khorram

state: CA

address: 1124  w carson st

institute: The lundquist Institute at UCLA medical center

description:
Library name: Sample 12 (Small)
Processed DATA-Small.xls

data_processing:
FastQC was used to check quality of reads
(1)Discard the read pair if either one read contains adapter contamination;
(2)Discard the read pair if more than 10% of bases are uncertain in either one read;
(3)Discard the read pair if the proportion of low quality bases is over 50% in either one read.
Hisat2 and featureCounts was used to align and count reads.
DeSeq2 was used for differential expression analysis.
Assembly: hg18
Supplementary files format and content: Processed DATA-*.xls containing DESeq2 normalized counts

source_name: Leiomyoma

extract_protocol:
TRIzol
Performed by Novogene Corporation Inc.
Total RNA was used for library preparation using the QIAseq Large and Small RNA Library Kit (QIAGEN) according to the manufacturer’s protocol. Briefly, RNA was subjected to adapter ligation for both small and long RNA species, followed by reverse transcription and PCR amplification with unique sample indices. Libraries were purified and size-selected according to kit instructions and assessed for quality and concentration prior to sequencing.

value: Leiomyoma


title: K16(V2)(Small)

description:
Library name: Sample 9 (Small)
Processed DATA-Small.xls

value: vehicle


title: K17(TI2)(Small)

description:
Library name: Sample 10 (Small)
Processed DATA-Small.xls


title: K15(TI1)(Small)

description:
Library name: Sample 8 (Small)
Processed DATA-Small.xls


title: K14(V1)(Small)

description:
Library name: Sample 7 (Small)
Processed DATA-Small.xls


title: mt-EBFP2 HMC3 3D Culture, Control 1

description: Library name: M-s1


title: K21(TI3)(Large)

description:
Library name: Sample 6 (Large)
Processed DATA-Large.xls


title: K20(V3)(Small)

description:
Library name: Sample 11 (Small)
Processed DATA-Small.xls


title: K20(V3)(Large)

description:
Library name: Sample 5 (Large)
Processed DATA-Large.xls


title: K15(TI1)(Large)

description:
Library name: Sample 2 (Large)
Processed DATA-Large.xls


title: K16(V2)(Large)

description:
Library name: Sample 3 (Large)
Processed DATA-Large.xls


title: K14(V1)(Large)

description:
Library name: Sample 1 (Large)
Processed DATA-Large.xls


title: LNCaP-resistance-ATAC-R2

city: guagnzhou

first: Chenwei

last: Wu

address: 南方医科大学高层学生公寓1635

institute: 南方医科大学

description: Library name: sample4

library_source: genomic

data_processing:
The ATAC-seq reads were mapped to the hg38 human genome using bowtie2 (v2.2.5) with parameters “-X 2000 --very-sensitive-local -no-discordant”. PCR duplicated reads were removed by sambamba (v0.6.6). SAM files were converted to BAM files using samtools (v1.6). MACS2 (v2.2.6) was used to call peaks with -q 0.05. Deeptools (v3.5.2) was used to convert BAM files to Bigwig files and generate the profile plot and heatmap. Homer (v4.11) was used to perform motif analysis. ATAC-seq signal tracks were visualized by IGV (v2.16.2). R package EdgeR (3.22.5) was used to perform differential peaks analysis.
Assembly: hg38
Supplementary files format and content: Library normalized bigWig files were provided for all samples

molecule: genomic DNA

source_name: prostate

growth_protocol: LNCaP, LNCaP-resistance cells were cultured in RPMI-1640 supplemented with 10% fetal bovine serum (FBS) and antibiotics in humidified atmosphere with 5% CO2 at 37℃.

extract_protocol:
ATAC-seq was performed using theHyperactive ATAC-Seq Library Prep Kit for Illumina (Vazyme, TD711). according to the manufacturer’s instructions. Briefly, 1x105 of cells were used for the  experiment, cells were collected, washed, Following lysis, the cells were subjected to the tagmentation reaction using the MIX reaction cocktail from the kit. The resulting fragmented DNA products were isolated via ATAC DNA Extract Beads. Library amplification was then carried out, utilizing the manufacturer's recommended Library Amplification Reaction Mix, typically consisting of 12 amplification cycles. The amplified libraries were purified using ATAC DNA Clean Beads, and their concentration was precisely determined using the Equalbit 1× dsDNA HS (High Sensitivity) Assay Kit (Vazyme, EQ121).
For ATAC-seq library amplification, puried DNA was mixed with 2 × CAM and uniquely barcoded i5 and i7 primers following PCR program and purified by DNA Selection Beads.

value: prostate

value: LNCaP-resistance

value: prostate cancer cell line

value: resistance cells

value: treat with enzalutamide

tag: batch


title: LNCaP-resistance-ATAC-R1

address: 南方医科大学高层学生公寓1635

description: Library name: sample3


title: LNCaP-ATAC-R2

address: 南方医科大学高层学生公寓1635

description: Library name: sample2

value: LNCaP

value: WT


title: LNCaP-35-RNA-2

address: 南方医科大学高层学生公寓1635

description:
Library name: sample4
Column name in LNCaP_gene_count.txt: LNCaP-35-2

data_processing:
RNA-seq reads were mapped to the human reference genome (hg38) human genome assembly using HISAT2 (v2.0.5) with default parameters.
Featurecounts (v1.5.0-p3) was used for counting the read numbers.
Assembly: hg38
Supplementary files format and content: tab-deliminated text files include raw gene counts

extract_protocol:
Total RNA was prepared using TRIzol reagent (Life Technologies) following manufacturer’s protocol
Library preparations were performed using the NEBNext Ultra RNA Library Prep Kit for illumina following manufacturer’s protocol


title: LNCaP-ATAC-R1

address: 南方医科大学高层学生公寓1635

description: Library name: sample1


title: LNCaP-RNA-1

address: 南方医科大学高层学生公寓1635

description:
Library name: sample1
Column name in LNCaP_gene_count.txt: LNCaP-1


title: LNCaP-RNA-2

address: 南方医科大学高层学生公寓1635

description:
Library name: sample2
Column name in LNCaP_gene_count.txt: LNCaP-2


title: V13M06-404_C1

city: Baltimore

first: Keri

last: Martinowich

state: Maryland

address: 855 N Wolfe St Suite 300

institute: Lieber Institute for Brain Development

description: Library name: V13M06-404_C1

data_processing:
FASTQ and image data files were pre-processed with the 10x SpaceRanger pipeline
Reads were aligned to reference genome GRCh38 (10x Genomics 2020 reference version)
Assembly: GRCh38
Supplementary files format and content: FASTQ and image data files were pre-processed with the 10x SpaceRanger pipeline with Aligned_fiducials.jpg, detected_tissue_image.jpg, scalefactors_json.json, tissue_hires_image.png, tissue_lowres_image.png, tissue_positions_list.csv, barcode, feature, and matrix files for each donor.
Library strategy: Spatial Transcriptomics

molecule: polyA RNA

source_name: pons

extract_protocol:
Hypothalamus was dissected from fresh frozen postmortem human tissue. 10 micron coronal sections were obtained by cryosectioning. Tissue was permeabilized and cDNA was synthesized to incorporate positional barcode.
Libraries were prepared using the Visium Spatial Gene Expression slides & kit according to manufacturer's instructions

value: pons


title: K17(TI2)(Large)

description:
Library name: Sample 4 (Large)
Processed DATA-Large.xls


title: V13M06-404_B1

description: Library name: V13M06-404_B1


title: V13M06-404_A1

description: Library name: V13M06-404_A1


title: V13M06-403_C1

description: Library name: V13M06-403_C1


title: V13M06-403_D1

description: Library name: V13M06-403_D1


title: V13M06-401_D1

description: Library name: V13M06-401_D1


title: V13M06-402_D1

description: Library name: V13M06-402_D1


title: V13M06-402_B1

description: Library name: V13M06-402_B1


title: V13M06-402_C1

description: Library name: V13M06-402_C1


title: V13M06-403_B1

description: Library name: V13M06-403_B1


title: V13M06-401_A1

description: Library name: V13M06-401_A1


title: LNCaP-35-RNA-1

address: 南方医科大学高层学生公寓1635

description:
Library name: sample3
Column name in LNCaP_gene_count.txt: LNCaP-35-1


title: V13M06-403_A1

description: Library name: V13M06-403_A1


title: V13M06-401_B1

description: Library name: V13M06-401_B1


title: V13M06-386_C1

description: Library name: V13M06-386_C1


title: V13M06-404_D1

description: Library name: V13M06-404_D1


title: V13M06-386_B1

description: Library name: V13M06-386_B1


title: V13M06-401_C1

description: Library name: V13M06-401_C1


title: V13M06-402_A1

description: Library name: V13M06-402_A1


title: V13M06-333_D1

description: Library name: V13M06-333_D1


title: V13M06-333_B1

description: Library name: V13M06-333_B1


title: V13M06-386_A1

description: Library name: V13M06-386_A1


title: V13M06-386_D1

description: Library name: V13M06-386_D1


title: V13M06-332_B1

description: Library name: V13M06-332_B1


title: V13M06-332_C1

description: Library name: V13M06-332_C1


title: V13M06-332_A1

description: Library name: V13M06-332_A1


title: V13M06-333_A1

description: Library name: V13M06-333_A1


title: V13M06-333_C1

description: Library name: V13M06-333_C1


title: V13F27-339_D1

description: Library name: V13F27-339_D1


title: V13M06-332_D1

description: Library name: V13M06-332_D1


title: V13M06-331_A1

description: Library name: V13M06-331_A1


title: V13F27-339_A1

description: Library name: V13F27-339_A1


title: V13M06-331_C1

description: Library name: V13M06-331_C1


title: V13F27-339_B1

description: Library name: V13F27-339_B1


title: V13B23-284_C1

description: Library name: V13B23-284_C1


title: V13M06-331_B1

description: Library name: V13M06-331_B1


title: V13F27-339_C1

description: Library name: V13F27-339_C1


title: V13B23-286_A1

description: Library name: V13B23-286_A1


title: V13B23-284_D1

description: Library name: V13B23-284_D1


title: V13B23-286_B1

description: Library name: V13B23-286_B1


title: V13B23-284_B1

description: Library name: V13B23-284_B1


title: V13M06-331_D1

description: Library name: V13M06-331_D1


title: V13B23-366_A1

description: Library name: V13B23-366_A1


title: Br1204

description: Library name: Br1204-V13F27-336_B1

data_processing:
FASTQ and image data files were pre-processed with the 10x SpaceRanger pipeline
Assembly: GRCh38
Supplementary files format and content: FASTQ and image data files were pre-processed with the 10x SpaceRanger pipeline with Aligned_fiducials.jpg, detected_tissue_image.jpg, scalefactors_json.json, tissue_hires_image.png, tissue_lowres_image.png, tissue_positions_list.csv, barcode, feature, and matrix files for each donor.
Library strategy: Spatial Transcriptomics

source_name: brain (dorsolateral prefrontal cortex)

extract_protocol:
DLPFC tissue blocks were dissected from fresh-frozen postmortem human brain tissue slabs. Coronal cryosections (10 µm thick) were mounted onto 10x Genomics Visium Spatial Transcriptomics arrays (v1, 3’ Gene Expression based on 3’ poly(A) capture-based chemistry). Sections were immunostained for nuclei (DAPI), neurons (NeuN), perineuronal nets (WFA), and vasculature (Claudin-5), and subsequently processed for spatial transcriptomics. Tissue permeabilization and cDNA synthesis incorporating positional barcodes were performed according to the manufacturer’s instructions (CG000239 Rev F).
Libraries were prepared using the Visium Spatial Gene Expression slides & kit according to manufacturer's instructions (CG000239 Rev F)

value: brain (dorsolateral prefrontal cortex)


title: V13B23-284_A1

description: Library name: V13B23-284_A1


title: Br1923

description: Library name: Br1923-V13F27-336_C1


title: Br8218

description: Library name: Br8218-V13F27-336_A1


title: Br5228

description: Library name: Br5228-V13M06-344_D1


title: Br1214

description: Library name: Br1214-V13M06-344_C1


title: Br2421

description: Library name: Br2421-V13F27-336_D1


title: Br5973

description: Library name: Br5973-V13M06-343_D1


title: Br5573

description: Library name: Br5573-V13M06-343_C1


title: Br6369

description: Library name: Br6369-V13M06-344_B1


title: Br5276

description: Library name: Br5276-V13M06-343_A1


title: Br6496

description: Library name: Br6496-V13M06-344_A1


title: Br8492

description: Library name: Br8492-V13M06-343_B1


title: Br8433

description: Library name: Br8433-V13M06-342_C1


title: Br2470

description: Library name: Br2470-V13M06-340_D1


title: Br1753

description: Library name: Br1753-V13F27-296_C1


title: Br5931

description: Library name: Br5931-V13M06-340_A1


title: Br8207

description: Library name: Br8207-V13M06-342_B1


title: Br5224

description: Library name: Br5224-V13M06-342_A1


title: Br6389

description: Library name: Br6389-V13F27-296_D1


title: Br1578

description: Library name: Br1578-V13F27-296_B1


title: Br2627

description: Library name: Br2627-V13F27-295_C1


title: Br1139

description: Library name: Br1139-V13F27-296_A1


title: Br8772

description: Library name: Br8772-V13F27-295_D1


title: Br8406

description: Library name: Br8406-V13M06-340_B1


title: Br1682

description: Library name: Br1682-V13M06-340_C1


title: Br1092

description: Library name: Br1092-V13F27-295_A1


title: Br8325

description: Library name: Br8325-V13F27-295_B1


title: Br5639

description: Library name: Br5639-V13F27-294_D1


title: Br8667

description: Library name: Br8667-V13M06-342_D1


title: Br5314

description: Library name: Br5314-V13F27-294_C1


title: Br5928

description: Library name: Br5928-V13F27-294_B1


title: Br6032

description: Library name: Br6032-V13F27-293_D1


title: Br5746

description: Library name: Br5746-V13F27-294_A1


title: Br5599

description: Library name: Br5599-V13F27-293_B1


title: Br5622

description: Library name: Br5622-V13F27-293_A1


title: Br5588

description: Library name: Br5588-V13F27-293_C1


title: Br6297

description: Library name: Br6297-V13M06-282_C1


title: Br6437

description: Library name: Br6437-V13M06-282_B1


title: Br5436

description: Library name: Br5436-V13M06-282_D1


title: Br6560

description: Library name: Br6560-V13M06-282_A1


title: Br5888

description: Library name: Br5888-V13M06-281_D1


title: Br2720

description: Library name: Br2720-V13M06-280_C1


title: Br5636

description: Library name: Br5636-V13M06-280_B1


title: Br5395

description: Library name: Br5395-V13M06-281_B1


title: Br2242

description: Library name: Br2242-V13M06-281_C1


title: Br5400

description: Library name: Br5400-V13M06-281_A1


title: Br8537

description: Library name: Br8537-V13M06-279_C1


title: Br8514

description: Library name: Br8514-V13M06-279_D1


title: Br1113

description: Library name: Br1113-V13M06-279_A1


title: Br6257

description: Library name: Br6257-V13M06-280_D1


title: Br5590

description: Library name: Br5590-V13M06-280_A1


title: Br6432

description: Library name: Br6432-V12D07-334_D1


title: Br5472

description: Library name: Br5472-V12D07-334_B1


title: Br2378

description: Library name: Br2378-V12F14-057_D1


title: Br5789

description: Library name: Br5789-V12D07-334_C1


title: Br1958

description: Library name: Br1958-V12F14-057_B1


title: Br2039

description: Library name: Br2039-V12F14-057_C1


title: Br5373

description: Library name: Br5373-V12D07-334_A1


title: Br8042

description: Library name: Br8042-V13M06-279_B1


title: D90 Hs-HAR1984 (Pt/Pt) -line 2

city: Durham

first: Federica

last: Mosti

state: NC

address: 213 Research Dr

institute: Duke University

description: Library name: sample8

library_source: transcriptomic single cell

data_processing:
The demultiplexing, barcoded processing, gene counting and aggregation were made using Cell Ranger software version 6.1.2
Assembly: hg38
Supplementary files format and content: Supplementary files format and content: excel files include average log2FC and average CPM for ctrl and CRISPR KI for each timepoint

source_name: Cortical organoids D90

extract_protocol:
Approximately 15,000 cells per channel (to give estimated recovery of 10,000 cells per channel) were loaded onto a Chromium Single Cell 3′ Chip (10x Genomics, PN-120236) and processed through the Chromium controller to generate single-cell gel beads in emulsion (GEMs).
scRNA-seq libraries were prepared with the Chromium Single Cell 3′ Library & Gel Bead Kit v.2 (10x Genomics, PN-120237).

treatment_protocol: Cells were then centrifuged at 300g for 7min and then filtered (Sysmex celltrix, 04-004-2326). Dissociated cells were resuspended in ice-cold PBS containing 0.04% BSA (VWR, 97061-420) at a concentration of 1,000 cells/μl, and approximately 15,000 cells per channel (to give estimated recovery of 10,000 cells per channel)

value: Cortical organoids D90

value: cortical organoids

value: Hs-HAR1984 (Pt/Pt) -line 2

value: CRISPR KI


title: D90 Hs-HAR1984 (Hs/Hs) -line 1

description: Library name: sample5

value: Hs-HAR1984 (Hs/Hs) -line 1

value: control


title: Br5182

description: Library name: Br5182-V12F14-053_C1


title: D90 Hs-HAR1984 (Hs/Hs) -line 2

description: Library name: sample6

value: Hs-HAR1984 (Hs/Hs) -line 2


title: Br2719

description: Library name: Br2719-V12F14-053_A1


title: Br5367

description: Library name: Br5367-V12F14-053_D1


title: D60 Hs-HAR1984 (Hs/Hs) -line 2

description: Library name: sample4

source_name: Cortical organoids D60

value: Cortical organoids D60


title: Br1526

description: Library name: Br1526-V12F14-057_A1


title: D60 Hs-HAR1984 (Pt/Pt) -line 2

description: Library name: sample2


title: D90 Hs-HAR1984 (Pt/Pt) -line 1

description: Library name: sample7

value: Hs-HAR1984 (Pt/Pt) -line 1


title: HEK293T, Dox_CBPIDR7, RNAseq, Rep2

city: Sheffield

first: Daniel

middle: Arun

last: Bose

state: South Yorkshire

address: University of Sheffield, Firth Court, Western Bank

department: Biosciences

country: United Kingdom

institute: The University of Sheffield

description:
Library name: HEK293T_CBPIDR7_RNAseq_Rep2
NC_RNA_IDR7_2

data_processing:
Following sequencing, Read 2 was discarded and downstream data analysis was performed using only Read 1, according to manufacturer’s recommendations for libraries prepared using the Quant-seq 3’ mRNA-Seq Library Prep kit.
Raw data quality was assessed using FastQC v0.11.9 and adapters were trimmed using Cutadapt v3.4 implemented in Trim Galore.
Reads were aligned using STAR (v2.7.6)
All downstream analysis was done in DESeq2 v1.40.2 in Bioconductor
Assembly: human genome assembly GRCh38
Supplementary files format and content: tab delimited text (.txt) file containing raw read counts for each sample

growth_protocol: HEK293T cells containing stable doxycycline inducible CBP-GFP, were cultured in complete DMEM (10% Tetracycline free FBS, 1% Penicillin-Streptomycin) including 2 mg/ml G418 (Generon) and 50ug/ml hygromycin (ThermoFisher)

extract_protocol:
RNA was extracted using TRIzol and chloroform, DNase treated to remove genomic DNA, then further purified with a subsequent phenol chloroform extraction and ethanol precipitation. RNA concentrations were measured using the broad range RNA Qubit kit (ThermoFisher).
RNA-seq libraries were prepared using the Quant-seq 3’ mRNA-Seq Library Prep kit according to the manufacturer’s instructions. In brief, 500 ng RNA was used per sample for cDNA synthesis, subsequent RNA removal and purification. qPCR was used to identify the optimal number of cycles for PCR amplification: between 16-23 depending upon sample (qPCR Add-on Kit, Lexogen) and the cDNA was then amplified accordingly, followed by purification of the libraries using magnetic beads.

treatment_protocol: Cell lines were treated with predetermined concentrations of Doxycycline (CBPwt, 25ng/ml; CBPdIDR6, 5ng/ml; CBPdIDR7, 25ng/ml) or DMSO to induce expression of CBP-GFP.

value: Human embryonic kidney

value: CBPdeltaIDR7-GFP, TRE3G, Tet3G transactivator

value: Doxycycline


title: HEK293T, Dox_CBPIDR7, RNAseq, Rep1

description:
Library name: HEK293T_CBPIDR7_RNAseq_Rep1
NC_RNA_IDR6_1


title: HEK293T, Dox_CBPwt, RNAseq, Rep2

description:
Library name: HEK293T_CBPwt_RNAseq_Rep2
NC_RNA_IDR6_2

value: CBPwt-GFP, TRE3G, Tet3G transactivator


title: HEK293T, Dox_CBPwt, RNAseq, Rep1

description:
Library name: HEK293T_CBPwt_RNAseq_Rep1
NC_RNA_CG_1


title: D60 Hs-HAR1984 (Hs/Hs) -line 1

description: Library name: sample3


title: HEK293T, Dox_CBPIDR6, RNAseq, Rep2

description:
Library name: HEK293T_CBPIDR6_RNAseq_Rep2
NC_RNA_IDR7_1

value: CBPdeltaIDR6-GFP, TRE3G, Tet3G transactivator


title: HEK293T, Dox_CBPwt_H3K27ac, IgG, Rep2

description: Library name: HEK293T_CBPwt_H3K27ac_IgG_Rep2

data_processing:
Raw data quality was assessed using FastQC v0.11.9 and adapters were trimmed using Cutadapt v3.4 implemented in Trim Galore.
Trimmed reads were aligned to two distinct reference genomes: Homo sapiens GRCh38 (Target genome) or S.cervisiae SacCer3 R64 (spike-in control genome) using bowtie2 v2.5.4(Langmead et al. 2009) in very sensitive local alignment mode (--local --very-sensitive-local --no-unal --no-mixed --no-discordant -q -I 10 -X 700 --dovetail -x), before read quality filtering using SAMTools v1.15.1
We used featureCounts v2.0.6 (Liao et al. 2014) to generate a count matrix for each sample containing the total number of reads uniquely mapping to the spike-in genome. This was used to calculate a scaling factor for each sample using DEseq2 v1.40.2
To enable direct quantitative comparison of signal intensity across samples, normalized BigWig files were generated in MACS3 v3.0.0b1
H3K27ac enriched peaks were called using MACS3 and peak intensity values from spike-in normalized BigWig files were then used for inter-sample comparisons and differential analyses using a local installation of DeepTools v3.5.3
Assembly: Homo sapiens GRCh38 and Saccharomyces cerevisiae SacCer3 R64
Supplementary files format and content: Spike-in and RPM normalised bigWig files and H3K27ac enriced narrowPeak files.
Library strategy: CUT&Run

extract_protocol:
100,000 cells per reaction or input sample were harvested and DNA fragments were prepared using the CST CUT&RUN Assay kit according to the manufacturer’s instructions. In brief, 100,000 cells per reaction were harvested, washed and bound to concanavalin A coated magnetic beads. Antibodies for each reaction were added (0.5 µg IgG: Rabbit (DA1E) IgG (CST, part of CUT&RUN assay kit); 1 µg H3K27ac: Histone H3K27ac (Active Motif)) and incubated overnight at 4oC. pAG-MNase was added to each tube and incubated for 1 hour, then activated through the addiction of 3 mM CaCl2 for 30 minutes at 4oC. Stop buffer was added, supplemented with 50 pg Spike-in DNA per sample (CST, part of CUT&RUN assay kit) and DNA fragments were eluted from the beads. DNA was then purified by phenol:chloroform extraction and ethanol precipitation and resuspended in 50 µl 1x TE buffer.
CUT&RUN DNA fragments were used to prepare libraries using the NEBNext Ultra II Library prep kit as described for ChIP-seq with the following changes. Adaptors were diluted for 1:10 for non-input samples and were cleaned up without size selection. Between 10-14 cycles of PCR were used for adaptor ligation dependent upon starting DNA concentration.


title: HEK293T, Tet3G_CBP_H3K27ac, Input, Rep2

description: Library name: HEK293T_CBPtet3g_H3K27ac_input_Rep2

value: Tet3G transactivator

value: DMSO


title: HEK293T, Dox_CBPIDR6_H3K27ac, IgG, Rep2

description: Library name: HEK293T_CBPIDR6_H3K27ac_IgG_Rep2


title: HEK293T, Tet3G_CBP_H3K27ac, IgG, Rep2

description: Library name: HEK293T_CBPtet3g_H3K27ac_IgG_Rep2


title: HEK293T, Dox_CBPIDR7_H3K27ac, IgG, Rep2

description: Library name: HEK293T_CBPIDR7_H3K27ac_IgG_Rep2


title: HEK293T, Dox_CBPIDR6, RNAseq, Rep1

description:
Library name: HEK293T_CBPIDR6_RNAseq_Rep1
NC_RNA_CG_2


title: HEK293T, Dox_CBPwt_H3K27ac, Input, Rep2

description: Library name: HEK293T_CBPwt_H3K27ac_input_Rep2


title: HEK293T, Dox_CBPIDR7_H3K27ac, Input, Rep2

description: Library name: HEK293T_CBPIDR7_H3K27ac_input_Rep2


title: D60 Hs-HAR1984 (Pt/Pt) -line 1

description: Library name: sample1


title: HEK293T, Dox_CBPwt_H3K27ac, CUTRUN, Rep2

description: Library name: HEK293T_CBPwt_H3K27ac_Rep2


title: HEK293T, Dox_CBPIDR7_H3K27ac, IgG, Rep1

description: Library name: HEK293T_CBPIDR7_H3K27ac_IgG_Rep1


title: HEK293T, Tet3G_CBP_H3K27ac, CUTRUN, Rep2

description: Library name: HEK293T_CBPtet3g_H3K27ac_Rep2


title: HEK293T, Dox_CBPIDR6_H3K27ac, IgG, Rep1

description: Library name: HEK293T_CBPIDR6_H3K27ac_IgG_Rep1


title: HEK293T, Dox_CBPIDR7_H3K27ac, CUTRUN, Rep2

description: Library name: HEK293T_CBPIDR7_H3K27ac_Rep2


title: HEK293T, Dox_CBPIDR6_H3K27ac, Input, Rep2

description: Library name: HEK293T_CBPIDR6_H3K27ac_input_Rep2


title: HEK293T, Dox_CBPIDR6_H3K27ac, CUTRUN, Rep2

description: Library name: HEK293T_CBPIDR6_H3K27ac_Rep2


title: HEK293T, Dox_CBPwt_H3K27ac, IgG, Rep1

description: Library name: HEK293T_CBPwt_H3K27ac_IgG_Rep1


title: HEK293T, Tet3G_CBP_H3K27ac, Input, Rep1

description: Library name: HEK293T_CBPtet3g_H3K27ac_input_Rep1


title: HEK293T, Dox_CBPIDR7_H3K27ac, Input, Rep1

description: Library name: HEK293T_CBPIDR7_H3K27ac_input_Rep1


title: HEK293T, Dox_CBPIDR6_H3K27ac, Input, Rep1

description: Library name: HEK293T_CBPIDR6_H3K27ac_input_Rep1


title: HEK293T, Dox_CBPwt_H3K27ac, Input, Rep1

description: Library name: HEK293T_CBPwt_H3K27ac_input_Rep1


title: HEK293T, Tet3G_CBP_H3K27ac, IgG, Rep1

description: Library name: HEK293T_CBPtet3g_H3K27ac_IgG_Rep1


title: HEK293T, Dox_CBPwt_H3K27ac, CUTRUN, Rep1

description:
Library name: HEK293T_CBPwt_H3K27ac_Rep1
processed files from both replicates


title: HEK293T, Tet3G_CBP_GFP, Input, Rep2

data_processing:
ChIP-seq libraries were sequenced using paired-end 150 sequencing on an Illumina NovaSeq 6000 platform (Novogene).
Raw data quality was assessed using FastQC v0.11.9 and adapters were trimmed using Cutadapt v3.4 implemented in Trim Galore.
Trimmed reads were aligned to human genome assembly GRCh38 using Burrows-Wheeler Alignment (BWA) v0.7.17-r1188
Replicates were merged and duplicate reads removed using Picard v2.27.4 before read quality filtering using SAMTools v1.15.1
BigWig files were generated by normalizing per million reads mapped (RPM) and converting to bedgraph format with Bedtools v2.30.0 then to BigWig format using UCSC utilities (bedGraphToBigWig v377)
ChIPseq peaks were called on filtered data using MACS2 v2.2.7.1.
All initial processing, alignment and peak calling was completed as part of the Nextflow ChIP-Seq pipeline v2.00
Subsequent analysis was done using Bedtools v2.31.0 and DeepTools v3.5.5.
Assembly: human genome assembly GRCh38
Supplementary files format and content: bigWig, broadpeak

extract_protocol:
Cells were crosslinked at room temperature for 10 minutes using formaldehyde (1.1 % final). Chromatin was sheared to 150 - 300 bp fragments, using the Bioruptor Pico (Diagenode).
Samples were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645) following manufacturers instructions. Libraries were amplified using 13 cycles of PCR and post amplification libraries were cleaned up and size seletcted using AMPure XP beads (Beckman Coulter). Libraries were validated using a TapeStation (Agilent) using a High Sensitivity DNA ScreenTape.


title: HEK293T, Tet3G_CBP_H3K27ac, CUTRUN, Rep1

description:
Library name: HEK293T_CBPtet3g_H3K27ac_Rep1
processed files from both replicates


title: HEK293T, Dox_CBPIDR7_GFP, Input, Rep2


title: HEK293T, Dox_CBPwt_GFP, Input, Rep2


title: HEK293T, Dox_CBPIDR7_GFP, ChIP, Rep2

description:
KG_IDR7_GFP_IP.bigWig
KG_IDR7_GFP_IP_peaks.broadPeak


title: HEK293T, Tet3G_CBP_GFP, ChIP, Rep2

description:
KG_PT_CBP_IP.bigWig
KG_PT_CBP_IP_peaks.broadPeak


title: HEK293T, Dox_CBPIDR7_H3K27ac, CUTRUN, Rep1

description:
Library name: HEK293T_CBPIDR7_H3K27ac_Rep1
processed files from both replicates


title: HEK293T, Dox_CBPIDR6_GFP, ChIP, Rep2

description:
KG_IDR6_GFP_IP.bigWig
KG_IDR6_GFP_IP_peaks.broadPeak


title: HEK293T, Dox_CBPIDR6_GFP, Input, Rep1


title: HEK293T, Dox_CBPIDR7_GFP, Input, Rep1


title: HEK293T, Dox_CBPwt_GFP, ChIP, Rep2

description:
KG_CBP_GFP_IP.bigWig
KG_CBP_GFP_IP_peaks.broadPeak


title: HEK293T, Tet3G_CBP_GFP, Input, Rep1


title: HEK293T, Dox_CBPwt_GFP, Input, Rep1


title: HEK293T, Dox_CBPIDR7_GFP, ChIP, Rep1


title: HEK293T, Dox_CBPIDR6_GFP, Input, Rep2


title: left cardiac atrium, sinus rhythm, input, 20, vl0340

city: LONDON

first: Adrian

last: Rodriguez

state: London

address: 4 Newark St

department: Blizard Institute - Genomics and Child Health

institute: Queen Mary University of London

data_processing:
Libraries sequencing depth varied between 50 and 25 million reads. To facilitate processing of large fastq files, a subset were subsampled using the Seqtk tool before alignment (file name ending in "_50M.fastq"). Aligned BAM files were obtained with Burrows-Wheeler aligner 0.7.17 (default parameters) (LI et al., 2010).
Aligned BAM files were obtained with Burrows-Wheeler aligner 0.7.17 (default parameters) (LI et al., 2010)
Significant histone mark-enriched regions were identified by normalising the ChIP mapped reads over the input for each sample. BAM files were first processed to remove multi-mapping reads and sub-sampled to 20-50 million reads to normalize for sequencing depth across samples. MACS2 callPeak function was run (default parameters) to generate narrowPeak files for called peaks.
Supplementary files format and content: MACS2 peak files for each ChIP and input combination (.narrowPeak extension)

source_name: left cardiac tissue

extract_protocol:
Samples were weighed and crosslinked when taken from -80C storage. Fixation of protein-DNA interactions was achieved through immersion in 1% formaldehyde solution, where tissue samples were minced with a razor into pieces no bigger than 0.5cm3. 2.5M glycine was then added to quench the formaldehyde and tissue was rinsed with PBS. After homogenization of cross-linked tissues in a dounce tissue grinder, dounced suspensions were centrifuged and pellets were resuspended in three serial lysis buffers according to published protocols (SCHMIDT et al., 2009).  Chromatin was fragmented to 200-400bp fragments on a QSONICA-Q500 sonicator fitted with a 4417 (1/16 inch) tip. ChIP experiments were performed with chromatin obtained from 25-50 mg of atrial tissue and antibodies against H3K27ac (Abcam ab479), for the totality of samples, and H3K4me3 (Millipore CMA304) for a subset of these.
Illumina sequencing libraries were prepared from ChIP-enriched DNA using ThruPLEX DNA-seq libaray preparation kit with up to 10ng of input DNA and 8-15 PCR cycles. Libraries were purified using the AMPure XP Cleanup beads to enrich for fragments over 200bp and pooled in equimolar concentrations before sequencing on Illumina NovaSeq Instruments.

treatment_protocol: Adult human left and right atrial appendages were collected from patients undergoing open-heart surgeries. Samples were initially flash frozen in RNAlater at the time of removal, hand-delivered on dry ice and stored at -80¬∞C until further use for the current study. Processing of samples was performed across four different experimental batches. Samples were randomised in each experimental batch to ensure even distribution of biological groups (anatomical side and disease status) using the R/Bioconductor package OSAT (YAN et al., 2012).

value: left cardiac tissue

tag: developmental stage

value: adult

tag: individual

tag: disease

value: sinus rhythm

tag: antibody

value: input


title: HEK293T, Dox_CBPIDR6_H3K27ac, CUTRUN, Rep1

description:
Library name: HEK293T_CBPIDR6_H3K27ac_Rep1
processed files from both replicates


title: HEK293T, Dox_CBPIDR6_GFP, ChIP, Rep1


title: left cardiac atrium, atrial fibrillation, input, 18, vl0334

value: atrial fibrillation


title: left cardiac atrium, sinus rhythm, H3K27ac, 27, vl0328


title: left cardiac atrium, sinus rhythm, input, 27, vl0335


title: right cardiac atrium, atrial fibrillation, input, 18, vl0333

source_name: right cardiac tissue

value: right cardiac tissue


title: HEK293T, Tet3G_CBP_GFP, ChIP, Rep1


title: right cardiac atrium, sinus rhythm, input, 20, vl0267


title: left cardiac atrium, atrial fibrillation, H3K4me3, 18, vl0323


title: right cardiac atrium, atrial fibrillation, input, 313, vl0266


title: right cardiac atrium, atrial fibrillation, input, 2231, vl0339


title: left cardiac atrium, atrial fibrillation, input, 313, vl0263


title: HEK293T, Dox_CBPwt_GFP, ChIP, Rep1


title: right cardiac atrium, atrial fibrillation, H3K27ac, 18, vl0326


title: left cardiac atrium, atrial fibrillation, H3K27ac, 18, vl0327


title: right cardiac atrium, sinus rhytm, H3K27ac, 20, vl0261


title: right cardiac atrium, atrial fibrillation, H3K27ac, 313, vl0260


title: left cardiac atrium, atrial fibrillation, H3K27ac, 313, vl0257


title: right cardiac atrium,  sinus rhythm, H3K4me3, 20, vl0253


title: left cardiac atrium, atrial fibrillation, input, 2231, vl0262


title: right cardiac atrium, atrial fibrillation, H3K4me3, 31, vl0250


title: right cardiac atrium, atrial fibrillation, input, 31, vl0264


title: left cardiac atrium, atrial fibrillation, H3K27ac, 2231, vl0256


title: left cardiac atrium, atrial fibrillation, input, 31, vl0231


title: right cardiac atrium, atrial fibrillation, H3K4me3, 313, vl0252


title: right cardiac atrium, atrial fibrillation, input, 47, vl0227


title: left cardiac atrium, atrial fibrillation, input, 47, vl0228


title: left cardiac atrium, sinus rhythm , iput, 45, vl0229


title: left cardiac atrium, atrial fibrillation, H3K4me3, 47, vl0220


title: left cardiac atrium, atrial fibrillation, H3K4me3, 2231, vl0248


title: left cardiac atrium, atrial fibrillation, H3K4me3, 313, vl0249


title: right cardiac atrium, atrial fibrillation, H3K27ac, 31, vl0258


title: left cardiac atrium, atrial fibrillation, H3K4me3, 31, vl0223


title: left cardiac atrium, sinus rhythm, H3K27ac, 20, vl0217


title: left cardiac atrium, atrial fibrillation, H3K27ac, 31, vl0215


title: right cardiac atrium, sinus rhythm, input, 33, vl0234


title: right cardiac atrium, sinus rhythm, H3K27ac, 33, vl0218


title: right cardiac atrium, atrial fibrillation, H3K27ac, 2231, vl0214


title: left cardiac, atrium, atrial fibrillation, H3K27ac, 47, vl0212


title: right cardiac atrium, atrial fibrillation, H3K27ac, 47, vl0211


title: right cardiac atrium, atrial fibrillation, input, 28, vl0041


title: left cardiac atrium, atrial fibrillation, input, 28, vl0040


title: left cardiac atrium, atrial fibrillation,  input, 25, vl0036


title: left cardiac atrium, sinus rhythm, H3K27ac, 45, vl0213


title: right cardiac atrium, sinus rhythm, input, 12b, vl0035


title: left cardiac atrium, sinus rhythm, input, 27, vl0039


title: right cardiac atrium, atrial fibrillation, input, 25, vl0037


title: left cardiac atrium, atrial fibrillation, H3K27ac, 28, vl0032


title: right cardiac atrium, atrial fibrillation, H3K27ac, 25, vl0028


title: right cardiac atrium, sinus rhythm, H3K27ac, 27, vl0031


title: left cardiac atrium, sinus rhythm, H3K27ac, 12b, vl0026


title: hapo_newborn_cordblood_S2771

type: genomic

city: Chicago

first: Jami

middle: L

last: Josefson

state: Illinois

address: 225 E Chicago Avenue

institute: Ann & Robert H. Lurie Children's Hospital of Chicago

description: Illumina Infinium MethylationEPIC BeadChip (850K chip)

hyb_protocol: Genome-wide methylation profiling was conducted with the Infinium MethylationEPIC BeadChip version 1.0 (Illumina, Inc., San Diego, California) array, which interrogates > 850,000 CpG sites at singlenucleotide resolution

scan_protocol: BeadChips were scanned with an Illumina iScan

data_processing:
minfi package in R environment (version 1.40.0)
processed value definition: M values transformed from beta (HAPO_GA_illumina_methylation.xlsx linked to the Series record)

label: Cy3, Cy5

source_name: newborn cordblood

label_protocol: Standard Illumina Protocol

extract_protocol: DNA was extracted from whole blood that was stored at -80 C and was quantified with the Qubit Fluorometer (Thermo Fisher Scientific, Waltham, Massachusetts)

tag: Sex

value: Female

value: cord clood

value: newborn

value: whole blood cells


title: right cardiac atrium, atrial fibrillation, H3K27ac, 28, vl0033


title: left cardiac atrium, atrial fibrillation, H3K27ac, 25, vl0028


title: hapo_newborn_cordblood_S2731


title: hapo_newborn_cordblood_S2751


title: hapo_newborn_cordblood_S2717

value: Male


title: hapo_newborn_cordblood_S2707


title: hapo_newborn_cordblood_S2729


title: left cardiac atrium, sinus rhythm, input, 12b, vl0034


title: hapo_newborn_cordblood_S2677


title: hapo_newborn_cordblood_S2561


title: hapo_newborn_cordblood_S2693


title: hapo_newborn_cordblood_S2594


title: right cardiac atrium, sinus rhythm, H3K27ac, 12b, vl0027


title: hapo_newborn_cordblood_S2657


title: hapo_newborn_cordblood_S2539


title: hapo_newborn_cordblood_S2557


title: hapo_newborn_cordblood_S2552


title: hapo_newborn_cordblood_S2502


title: hapo_newborn_cordblood_S2550


title: hapo_newborn_cordblood_S2516


title: hapo_newborn_cordblood_S2417


title: hapo_newborn_cordblood_S2397


title: hapo_newborn_cordblood_S2348


title: hapo_newborn_cordblood_S2391


title: hapo_newborn_cordblood_S2451


title: hapo_newborn_cordblood_S2684


title: hapo_newborn_cordblood_S2309


title: hapo_newborn_cordblood_S2385


title: hapo_newborn_cordblood_S2479


title: hapo_newborn_cordblood_S2489


title: hapo_newborn_cordblood_S2339


title: hapo_newborn_cordblood_S2335


title: hapo_newborn_cordblood_S2325


title: hapo_newborn_cordblood_S2329


title: hapo_newborn_cordblood_S2262


title: hapo_newborn_cordblood_S2377


title: hapo_newborn_cordblood_S2267


title: hapo_newborn_cordblood_S2257


title: hapo_newborn_cordblood_S2255


title: hapo_newborn_cordblood_S2272


title: hapo_newborn_cordblood_S2226


title: hapo_newborn_cordblood_S2207


title: hapo_newborn_cordblood_S2277


title: hapo_newborn_cordblood_S2206


title: hapo_newborn_cordblood_S2290


title: hapo_newborn_cordblood_S2215


title: hapo_newborn_cordblood_S2196


title: hapo_newborn_cordblood_S2254


title: hapo_newborn_cordblood_S2182


title: hapo_newborn_cordblood_S2176


title: hapo_newborn_cordblood_S2154


title: hapo_newborn_cordblood_S2151


title: hapo_newborn_cordblood_S2136


title: hapo_newborn_cordblood_S2131


title: hapo_newborn_cordblood_S2120


title: hapo_newborn_cordblood_S2124


title: hapo_newborn_cordblood_S2117


title: hapo_newborn_cordblood_S2170


title: hapo_newborn_cordblood_S2047


title: hapo_newborn_cordblood_S2039


title: hapo_newborn_cordblood_S2020


title: hapo_newborn_cordblood_S2114


title: hapo_newborn_cordblood_S2029


title: hapo_newborn_cordblood_S2040


title: hapo_newborn_cordblood_S2024


title: hapo_newborn_cordblood_S2006


title: hapo_newborn_cordblood_S1993


title: hapo_newborn_cordblood_S2028


title: hapo_newborn_cordblood_S1994


title: hapo_newborn_cordblood_S1984


title: hapo_newborn_cordblood_S2153


title: hapo_newborn_cordblood_S1980


title: hapo_newborn_cordblood_S2084


title: hapo_newborn_cordblood_S2007


title: hapo_newborn_cordblood_S1978


title: hapo_newborn_cordblood_S1957


title: hapo_newborn_cordblood_S1964


title: hapo_newborn_cordblood_S1953


title: hapo_newborn_cordblood_S1929


title: hapo_newborn_cordblood_S1939


title: hapo_newborn_cordblood_S1902


title: hapo_newborn_cordblood_S1866


title: hapo_newborn_cordblood_S1894


title: hapo_newborn_cordblood_S1876


title: hapo_newborn_cordblood_S1846


title: hapo_newborn_cordblood_S1865


title: hapo_newborn_cordblood_S1841


title: hapo_newborn_cordblood_S1806


title: hapo_newborn_cordblood_S1773


title: hapo_newborn_cordblood_S1758


title: hapo_newborn_cordblood_S1897


title: hapo_newborn_cordblood_S1810


title: hapo_newborn_cordblood_S1780


title: hapo_newborn_cordblood_S1765


title: hapo_newborn_cordblood_S1856


title: hapo_newborn_cordblood_S1744


title: hapo_newborn_cordblood_S1703


title: hapo_newborn_cordblood_S1735


title: hapo_newborn_cordblood_S1687


title: hapo_newborn_cordblood_S1643


title: hapo_newborn_cordblood_S1686


title: hapo_newborn_cordblood_S1805


title: hapo_newborn_cordblood_S1647


title: hapo_newborn_cordblood_S1702


title: hapo_newborn_cordblood_S1776


title: hapo_newborn_cordblood_S1767


title: hapo_newborn_cordblood_S1628


title: hapo_newborn_cordblood_S1622


title: hapo_newborn_cordblood_S1620


title: hapo_newborn_cordblood_S1670


title: hapo_newborn_cordblood_S1608


title: hapo_newborn_cordblood_S1696


title: hapo_newborn_cordblood_S1631


title: hapo_newborn_cordblood_S1577


title: hapo_newborn_cordblood_S1574


title: hapo_newborn_cordblood_S1592


title: hapo_newborn_cordblood_S1550


title: hapo_newborn_cordblood_S1558


title: hapo_newborn_cordblood_S1618


title: hapo_newborn_cordblood_S1567


title: hapo_newborn_cordblood_S1541


title: hapo_newborn_cordblood_S1610


title: hapo_newborn_cordblood_S1539


title: hapo_newborn_cordblood_S1532


title: hapo_newborn_cordblood_S1526


title: hapo_newborn_cordblood_S1531


title: hapo_newborn_cordblood_S1595


title: hapo_newborn_cordblood_S1468


title: hapo_newborn_cordblood_S1482


title: hapo_newborn_cordblood_S1495


title: hapo_newborn_cordblood_S1497


title: hapo_newborn_cordblood_S1436


title: hapo_newborn_cordblood_S1433


title: hapo_newborn_cordblood_S1401


title: hapo_newborn_cordblood_S1404


title: hapo_newborn_cordblood_S1391


title: hapo_newborn_cordblood_S1370


title: hapo_newborn_cordblood_S1516


title: hapo_newborn_cordblood_S1443


title: hapo_newborn_cordblood_S1364


title: hapo_newborn_cordblood_S1351


title: hapo_newborn_cordblood_S1339


title: hapo_newborn_cordblood_S1349


title: hapo_newborn_cordblood_S1344


title: hapo_newborn_cordblood_S1374


title: hapo_newborn_cordblood_S1335


title: hapo_newborn_cordblood_S1359


title: hapo_newborn_cordblood_S1348


title: hapo_newborn_cordblood_S1343


title: hapo_newborn_cordblood_S1329


title: hapo_newborn_cordblood_S1291


title: hapo_newborn_cordblood_S1307


title: hapo_newborn_cordblood_S1306


title: hapo_newborn_cordblood_S1333


title: hapo_newborn_cordblood_S1322


title: hapo_newborn_cordblood_S1193


title: hapo_newborn_cordblood_S1281


title: hapo_newborn_cordblood_S1082


title: hapo_newborn_cordblood_S1087


title: hapo_newborn_cordblood_S1239


title: hapo_newborn_cordblood_S1085


title: hapo_newborn_cordblood_S1070


title: hapo_newborn_cordblood_S1271


title: hapo_newborn_cordblood_S1071


title: hapo_newborn_cordblood_S1064


title: hapo_newborn_cordblood_S1060


title: hapo_newborn_cordblood_S1059


title: hapo_newborn_cordblood_S1026


title: hapo_newborn_cordblood_S1018


title: hapo_newborn_cordblood_S0971


title: hapo_newborn_cordblood_S0985


title: hapo_newborn_cordblood_S1017


title: hapo_newborn_cordblood_S0968


title: hapo_newborn_cordblood_S0959


title: hapo_newborn_cordblood_S0954


title: hapo_newborn_cordblood_S0931


title: hapo_newborn_cordblood_S0908


title: hapo_newborn_cordblood_S0912


title: hapo_newborn_cordblood_S0941


title: hapo_newborn_cordblood_S0842


title: hapo_newborn_cordblood_S0887


title: hapo_newborn_cordblood_S0852


title: hapo_newborn_cordblood_S0885


title: hapo_newborn_cordblood_S0868


title: hapo_newborn_cordblood_S0739


title: hapo_newborn_cordblood_S0757


title: hapo_newborn_cordblood_S0831


title: hapo_newborn_cordblood_S0785


title: hapo_newborn_cordblood_S0804


title: hapo_newborn_cordblood_S0764


title: hapo_newborn_cordblood_S0812


title: hapo_newborn_cordblood_S0714


title: hapo_newborn_cordblood_S0737


title: hapo_newborn_cordblood_S0685


title: hapo_newborn_cordblood_S0688


title: hapo_newborn_cordblood_S0684


title: hapo_newborn_cordblood_S0728


title: hapo_newborn_cordblood_S0674


title: hapo_newborn_cordblood_S0653


title: hapo_newborn_cordblood_S0656


title: hapo_newborn_cordblood_S0666


title: hapo_newborn_cordblood_S0648


title: hapo_newborn_cordblood_S0649


title: hapo_newborn_cordblood_S0644


title: hapo_newborn_cordblood_S0637


title: hapo_newborn_cordblood_S0636


title: hapo_newborn_cordblood_S0631


title: hapo_newborn_cordblood_S0681


title: hapo_newborn_cordblood_S0625


title: hapo_newborn_cordblood_S0629


title: hapo_newborn_cordblood_S0605


title: hapo_newborn_cordblood_S0626


title: hapo_newborn_cordblood_S0606


title: hapo_newborn_cordblood_S0678


title: hapo_newborn_cordblood_S0614


title: hapo_newborn_cordblood_S0622


title: hapo_newborn_cordblood_S0613


title: hapo_newborn_cordblood_S0632


title: hapo_newborn_cordblood_S0611


title: hapo_newborn_cordblood_S0960


title: hapo_newborn_cordblood_S0581


title: hapo_newborn_cordblood_S0580


title: hapo_newborn_cordblood_S0583


title: hapo_newborn_cordblood_S0559


title: hapo_newborn_cordblood_S0553


title: hapo_newborn_cordblood_S0590


title: hapo_newborn_cordblood_S0594


title: hapo_newborn_cordblood_S0603


title: hapo_newborn_cordblood_S0576


title: hapo_newborn_cordblood_S0557


title: hapo_newborn_cordblood_S0550


title: hapo_newborn_cordblood_S0529


title: hapo_newborn_cordblood_S0577


title: hapo_newborn_cordblood_S0525


title: hapo_newborn_cordblood_S0520


title: hapo_newborn_cordblood_S0523


title: hapo_newborn_cordblood_S0502


title: hapo_newborn_cordblood_S0500


title: hapo_newborn_cordblood_S0494


title: hapo_newborn_cordblood_S0555


title: hapo_newborn_cordblood_S0480


title: hapo_newborn_cordblood_S0479


title: hapo_newborn_cordblood_S0490


title: hapo_newborn_cordblood_S0547


title: hapo_newborn_cordblood_S0470


title: hapo_newborn_cordblood_S0469


title: hapo_newborn_cordblood_S0471


title: hapo_newborn_cordblood_S0461


title: hapo_newborn_cordblood_S0467


title: hapo_newborn_cordblood_S0452


title: hapo_newborn_cordblood_S0454


title: hapo_newborn_cordblood_S0438


title: hapo_newborn_cordblood_S0486


title: hapo_newborn_cordblood_S0450


title: hapo_newborn_cordblood_S0439


title: hapo_newborn_cordblood_S0422


title: hapo_newborn_cordblood_S0410


title: hapo_newborn_cordblood_S0417


title: hapo_newborn_cordblood_S0432


title: hapo_newborn_cordblood_S0430


title: hapo_newborn_cordblood_S0388


title: hapo_newborn_cordblood_S0460


title: hapo_newborn_cordblood_S0401


title: hapo_newborn_cordblood_S0385


title: hapo_newborn_cordblood_S0372


title: hapo_newborn_cordblood_S0405


title: hapo_newborn_cordblood_S0371


title: hapo_newborn_cordblood_S0381


title: hapo_newborn_cordblood_S0379


title: hapo_newborn_cordblood_S0367


title: hapo_newborn_cordblood_S0364


title: hapo_newborn_cordblood_S0370


title: hapo_newborn_cordblood_S0352


title: hapo_newborn_cordblood_S0342


title: hapo_newborn_cordblood_S0428


title: hapo_newborn_cordblood_S0344


title: hapo_newborn_cordblood_S0337


title: hapo_newborn_cordblood_S0359


title: hapo_newborn_cordblood_S0341


title: hapo_newborn_cordblood_S0328


title: hapo_newborn_cordblood_S0325


title: hapo_newborn_cordblood_S0311


title: hapo_newborn_cordblood_S0334


title: hapo_newborn_cordblood_S0333


title: hapo_newborn_cordblood_S0315


title: hapo_newborn_cordblood_S0302


title: hapo_newborn_cordblood_S0280


title: hapo_newborn_cordblood_S0297


title: hapo_newborn_cordblood_S0278


title: hapo_newborn_cordblood_S0335


title: hapo_newborn_cordblood_S0270


title: hapo_newborn_cordblood_S0268


title: hapo_newborn_cordblood_S0233


title: hapo_newborn_cordblood_S0232


title: hapo_newborn_cordblood_S0255


title: hapo_newborn_cordblood_S0209


title: hapo_newborn_cordblood_S0239


title: hapo_newborn_cordblood_S0309


title: hapo_newborn_cordblood_S0196


title: hapo_newborn_cordblood_S0226


title: hapo_newborn_cordblood_S0182


title: hapo_newborn_cordblood_S0195


title: hapo_newborn_cordblood_S0105


title: hapo_newborn_cordblood_S0077


title: hapo_newborn_cordblood_S0143


title: hapo_newborn_cordblood_S0064


title: hapo_newborn_cordblood_S0047


title: hapo_newborn_cordblood_S0019


title: hapo_newborn_cordblood_S0023


title: hapo_newborn_cordblood_S0034


title: hapo_newborn_cordblood_S0042


title: hapo_newborn_cordblood_S0006


title: hapo_newborn_cordblood_S0001


title: hapo_newborn_cordblood_S0078


title: hapo_newborn_cordblood_N2969


title: hapo_newborn_cordblood_N2954


title: hapo_newborn_cordblood_N2857


title: hapo_newborn_cordblood_N2970


title: hapo_newborn_cordblood_N2829


title: hapo_newborn_cordblood_N2797


title: hapo_newborn_cordblood_N2788


title: hapo_newborn_cordblood_N2737


title: hapo_newborn_cordblood_N2763


title: hapo_newborn_cordblood_N2807


title: hapo_newborn_cordblood_N2691


title: hapo_newborn_cordblood_N2941


title: hapo_newborn_cordblood_N2880


title: hapo_newborn_cordblood_N2965


title: hapo_newborn_cordblood_N2748


title: hapo_newborn_cordblood_N2774


title: hapo_newborn_cordblood_N2644


title: hapo_newborn_cordblood_N2919


title: hapo_newborn_cordblood_N2632


title: hapo_newborn_cordblood_N2672


title: hapo_newborn_cordblood_N2615


title: hapo_newborn_cordblood_N2658


title: hapo_newborn_cordblood_N2608


title: hapo_newborn_cordblood_N2605


title: hapo_newborn_cordblood_N2628


title: hapo_newborn_cordblood_N2604


title: hapo_newborn_cordblood_N2674


title: hapo_newborn_cordblood_N2588


title: hapo_newborn_cordblood_N2573


title: hapo_newborn_cordblood_N2581


title: hapo_newborn_cordblood_N2566


title: hapo_newborn_cordblood_N2570


title: hapo_newborn_cordblood_N2562


title: hapo_newborn_cordblood_N2551


title: hapo_newborn_cordblood_N2552


title: hapo_newborn_cordblood_N2525


title: hapo_newborn_cordblood_N2518


title: hapo_newborn_cordblood_N2505


title: hapo_newborn_cordblood_N2526


title: hapo_newborn_cordblood_N2496


title: hapo_newborn_cordblood_N2486


title: hapo_newborn_cordblood_N2449


title: hapo_newborn_cordblood_N2435


title: hapo_newborn_cordblood_N2409


title: hapo_newborn_cordblood_N2425


title: hapo_newborn_cordblood_N2433


title: hapo_newborn_cordblood_N2488


title: hapo_newborn_cordblood_N2306


title: hapo_newborn_cordblood_N2304


title: hapo_newborn_cordblood_N2310


title: hapo_newborn_cordblood_N2368


title: hapo_newborn_cordblood_N2259


title: hapo_newborn_cordblood_N2283


title: hapo_newborn_cordblood_N2302


title: hapo_newborn_cordblood_N2227


title: hapo_newborn_cordblood_N2320


title: hapo_newborn_cordblood_N2228


title: hapo_newborn_cordblood_N2177


title: hapo_newborn_cordblood_N2230


title: hapo_newborn_cordblood_N2242


title: hapo_newborn_cordblood_N2105


title: hapo_newborn_cordblood_N2163


title: hapo_newborn_cordblood_N2098


title: hapo_newborn_cordblood_N2138


title: hapo_newborn_cordblood_N2093


title: hapo_newborn_cordblood_N2125


title: hapo_newborn_cordblood_N2081


title: hapo_newborn_cordblood_N2091


title: hapo_newborn_cordblood_N2070


title: hapo_newborn_cordblood_N2056


title: hapo_newborn_cordblood_N2041


title: hapo_newborn_cordblood_N2165


title: hapo_newborn_cordblood_N2005


title: hapo_newborn_cordblood_N1959


title: hapo_newborn_cordblood_N2077


title: hapo_newborn_cordblood_N2007


title: hapo_newborn_cordblood_N1941


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title: hapo_newborn_cordblood_N1854


title: hapo_newborn_cordblood_N1876


title: hapo_newborn_cordblood_N1936


title: hapo_newborn_cordblood_N1904


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title: hapo_newborn_cordblood_N2028


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title: hapo_newborn_cordblood_N1500


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title: hapo_newborn_cordblood_N1270


title: hapo_newborn_cordblood_N1250


title: hapo_newborn_cordblood_N1262


title: hapo_newborn_cordblood_N1240


title: hapo_newborn_cordblood_N1225


title: hapo_newborn_cordblood_N1217


title: hapo_newborn_cordblood_N1246


title: hapo_newborn_cordblood_N1230


title: hapo_newborn_cordblood_N1405


title: hapo_newborn_cordblood_N1264


title: hapo_newborn_cordblood_N1193


title: hapo_newborn_cordblood_N1194


title: hapo_newborn_cordblood_N1111


title: hapo_newborn_cordblood_N1149


title: hapo_newborn_cordblood_N1085


title: hapo_newborn_cordblood_N1123


title: hapo_newborn_cordblood_N1099


title: hapo_newborn_cordblood_N1105


title: hapo_newborn_cordblood_N1151


title: hapo_newborn_cordblood_N1078


title: hapo_newborn_cordblood_N1050


title: hapo_newborn_cordblood_N1039


title: hapo_newborn_cordblood_N1063


title: hapo_newborn_cordblood_N1041


title: hapo_newborn_cordblood_N0964


title: hapo_newborn_cordblood_N1028


title: hapo_newborn_cordblood_N0995


title: hapo_newborn_cordblood_N0932


title: hapo_newborn_cordblood_N0939


title: hapo_newborn_cordblood_N0925


title: hapo_newborn_cordblood_N0892


title: hapo_newborn_cordblood_N0888


title: hapo_newborn_cordblood_N0881


title: hapo_newborn_cordblood_N0885


title: hapo_newborn_cordblood_N0869


title: hapo_newborn_cordblood_N0860


title: hapo_newborn_cordblood_N0834


title: hapo_newborn_cordblood_N0828


title: hapo_newborn_cordblood_N0816


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title: hapo_newborn_cordblood_N0030


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title: hapo_newborn_cordblood_N0048


title: hapo_newborn_cordblood_L2004


title: hapo_newborn_cordblood_L1999


title: hapo_newborn_cordblood_L1996


title: hapo_newborn_cordblood_L1983


title: hapo_newborn_cordblood_L1992


title: hapo_newborn_cordblood_L1994


title: hapo_newborn_cordblood_L1970


title: hapo_newborn_cordblood_L1945


title: hapo_newborn_cordblood_L1958


title: hapo_newborn_cordblood_L1937


title: hapo_newborn_cordblood_L1982


title: hapo_newborn_cordblood_L1947


title: hapo_newborn_cordblood_L1948


title: hapo_newborn_cordblood_L1953


title: hapo_newborn_cordblood_L1943


title: hapo_newborn_cordblood_L1946


title: hapo_newborn_cordblood_L1932


title: hapo_newborn_cordblood_L1929


title: hapo_newborn_cordblood_L1961


title: hapo_newborn_cordblood_L1924


title: hapo_newborn_cordblood_L1927


title: hapo_newborn_cordblood_L1916


title: hapo_newborn_cordblood_L1918


title: hapo_newborn_cordblood_L1913


title: hapo_newborn_cordblood_L1881


title: hapo_newborn_cordblood_L1876


title: hapo_newborn_cordblood_L1873


title: hapo_newborn_cordblood_L1863


title: hapo_newborn_cordblood_L1871


title: hapo_newborn_cordblood_L1868


title: hapo_newborn_cordblood_L1854


title: hapo_newborn_cordblood_L1891


title: hapo_newborn_cordblood_L1849


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title: hapo_newborn_cordblood_L1826


title: hapo_newborn_cordblood_L1786


title: hapo_newborn_cordblood_L1805


title: hapo_newborn_cordblood_L1896


title: hapo_newborn_cordblood_L1831


title: hapo_newborn_cordblood_L1835


title: hapo_newborn_cordblood_L1798


title: hapo_newborn_cordblood_L1824


title: hapo_newborn_cordblood_L1811


title: hapo_newborn_cordblood_L1792


title: hapo_newborn_cordblood_L1783


title: hapo_newborn_cordblood_L1782


title: hapo_newborn_cordblood_L1780


title: hapo_newborn_cordblood_L1843


title: hapo_newborn_cordblood_L1767


title: hapo_newborn_cordblood_L1763


title: hapo_newborn_cordblood_L1762


title: hapo_newborn_cordblood_L1766


title: hapo_newborn_cordblood_L1761


title: hapo_newborn_cordblood_L1754


title: hapo_newborn_cordblood_L1752


title: hapo_newborn_cordblood_L1751


title: hapo_newborn_cordblood_L1745


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title: hapo_newborn_cordblood_L1716


title: hapo_newborn_cordblood_L1702


title: hapo_newborn_cordblood_L1696


title: hapo_newborn_cordblood_L1700


title: hapo_newborn_cordblood_L1690


title: hapo_newborn_cordblood_L1668


title: hapo_newborn_cordblood_L1631


title: hapo_newborn_cordblood_L1750


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title: hapo_newborn_cordblood_L1651


title: hapo_newborn_cordblood_L1649


title: hapo_newborn_cordblood_L1656


title: hapo_newborn_cordblood_L1582


title: hapo_newborn_cordblood_L1718


title: hapo_newborn_cordblood_L1587


title: hapo_newborn_cordblood_L1610


title: hapo_newborn_cordblood_L1581


title: hapo_newborn_cordblood_L1599


title: hapo_newborn_cordblood_L1600


title: hapo_newborn_cordblood_L1565


title: hapo_newborn_cordblood_L1598


title: hapo_newborn_cordblood_L1609


title: hapo_newborn_cordblood_L1639


title: hapo_newborn_cordblood_L1545


title: hapo_newborn_cordblood_L1538


title: hapo_newborn_cordblood_L1535


title: hapo_newborn_cordblood_L1562


title: hapo_newborn_cordblood_L1533


title: hapo_newborn_cordblood_L1523


title: hapo_newborn_cordblood_L1521


title: hapo_newborn_cordblood_L1561


title: hapo_newborn_cordblood_L1548


title: hapo_newborn_cordblood_L1512


title: hapo_newborn_cordblood_L1546


title: hapo_newborn_cordblood_L1537


title: hapo_newborn_cordblood_L1496


title: hapo_newborn_cordblood_L1499


title: hapo_newborn_cordblood_L1472


title: hapo_newborn_cordblood_L1446


title: hapo_newborn_cordblood_L1471


title: hapo_newborn_cordblood_L1491


title: hapo_newborn_cordblood_L1450


title: hapo_newborn_cordblood_L1454


title: hapo_newborn_cordblood_L1455


title: hapo_newborn_cordblood_L1439


title: hapo_newborn_cordblood_L1449


title: hapo_newborn_cordblood_L1518


title: hapo_newborn_cordblood_L1438


title: hapo_newborn_cordblood_L1445


title: hapo_newborn_cordblood_L1433


title: hapo_newborn_cordblood_L1479


title: hapo_newborn_cordblood_L1436


title: hapo_newborn_cordblood_L1431


title: hapo_newborn_cordblood_L1428


title: hapo_newborn_cordblood_L1410


title: hapo_newborn_cordblood_L1415


title: hapo_newborn_cordblood_L1414


title: hapo_newborn_cordblood_L1392


title: hapo_newborn_cordblood_L1382


title: hapo_newborn_cordblood_L1426


title: hapo_newborn_cordblood_L1406


title: hapo_newborn_cordblood_L1380


title: hapo_newborn_cordblood_L1378


title: hapo_newborn_cordblood_L1375


title: hapo_newborn_cordblood_L1373


title: hapo_newborn_cordblood_L1359


title: hapo_newborn_cordblood_L1354


title: hapo_newborn_cordblood_L1329


title: hapo_newborn_cordblood_L1343


title: hapo_newborn_cordblood_L1363


title: hapo_newborn_cordblood_L1341


title: hapo_newborn_cordblood_L1358


title: hapo_newborn_cordblood_L1368


title: hapo_newborn_cordblood_L1322


title: hapo_newborn_cordblood_L1316


title: hapo_newborn_cordblood_L1330


title: hapo_newborn_cordblood_L1315


title: hapo_newborn_cordblood_L1302


title: hapo_newborn_cordblood_L1299


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title: hapo_newborn_cordblood_L1348


title: hapo_newborn_cordblood_L1372


title: hapo_newborn_cordblood_L1291


title: hapo_newborn_cordblood_L1264


title: hapo_newborn_cordblood_L1272


title: hapo_newborn_cordblood_L1262


title: hapo_newborn_cordblood_L1276


title: hapo_newborn_cordblood_L1283


title: hapo_newborn_cordblood_L1271


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title: hapo_newborn_cordblood_L1255


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title: hapo_newborn_cordblood_L1239


title: hapo_newborn_cordblood_L1240


title: hapo_newborn_cordblood_L1251


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title: hapo_newborn_cordblood_L1235


title: hapo_newborn_cordblood_L1250


title: hapo_newborn_cordblood_L1249


title: hapo_newborn_cordblood_L1233


title: hapo_newborn_cordblood_L1220


title: hapo_newborn_cordblood_L1213


title: hapo_newborn_cordblood_L1210


title: hapo_newborn_cordblood_L1211


title: hapo_newborn_cordblood_L1206


title: hapo_newborn_cordblood_L1205


title: hapo_newborn_cordblood_L1193


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title: hapo_newborn_cordblood_L1230


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title: hapo_newborn_cordblood_L1179


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title: hapo_newborn_cordblood_L1212


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title: hapo_newborn_cordblood_L1165


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title: hapo_newborn_cordblood_L1132


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title: hapo_newborn_cordblood_L1151


title: hapo_newborn_cordblood_L1141


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title: hapo_newborn_cordblood_K2500


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title: hapo_newborn_cordblood_K2522


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title: hapo_newborn_cordblood_K2444


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title: hapo_newborn_cordblood_K2533


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title: hapo_newborn_cordblood_K2430


title: hapo_newborn_cordblood_K2423


title: hapo_newborn_cordblood_K2427


title: hapo_newborn_cordblood_K2422


title: hapo_newborn_cordblood_K2431


title: hapo_newborn_cordblood_K2419


title: hapo_newborn_cordblood_K2410


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title: hapo_newborn_cordblood_K2412


title: hapo_newborn_cordblood_K2392


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title: hapo_newborn_cordblood_K2391


title: hapo_newborn_cordblood_K2352


title: hapo_newborn_cordblood_K2363


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title: hapo_newborn_cordblood_K2351


title: hapo_newborn_cordblood_K2365


title: hapo_newborn_cordblood_K2355


title: hapo_newborn_cordblood_K2348


title: hapo_newborn_cordblood_K2338


title: hapo_newborn_cordblood_K2350


title: hapo_newborn_cordblood_K2322


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title: hapo_newborn_cordblood_K2236


title: hapo_newborn_cordblood_K2221


title: hapo_newborn_cordblood_K2234


title: hapo_newborn_cordblood_K2200


title: hapo_newborn_cordblood_K2205


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title: hapo_newborn_cordblood_K1996


title: hapo_newborn_cordblood_K2028


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