title: C57Bl/6 YFP+ ALM single nucleus DLX2.0-H2B-SYFP2-BC-37

description:
Library name: AB-S40324_E1-50_GCTCAACCAA-CGCATTATCA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus DLX2.0-H2B-SYFP2-BC-36

status: Public on Feb 18 2026

type: SRA

city: Seattle

first: John

middle: Kenneth

last: Mich

state: WA

address: 615 Westlake Ave N

country: USA

institute: Allen Institute for Brain Science

description:
Library name: AB-S40324_E1-50_AAGTACTCGA-TGCGATTAGA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.

library_source: transcriptomic single cell

data_processing:
Illumina NovaSeq 6000 on-board base-calling protocol
Alignment with STAR v2.7.2a (https://github.com/alexdobin/STAR)
Assembly: mm10
Supplementary files format and content: Nuclei_SSv4_GEX_2501.csv.gz (for all three datasets; 2501 samples)

molecule: polyA RNA

organism: Mus musculus

source_name: anterior lateral motor cortex nucleus

extract_protocol:
SMART-seq v4 protocol (Takara)
Nextera XT DNA Library Preparation Kit (Illumina)

tag: tissue

value: anterior lateral motor cortex nucleus

tag: cell type

value: sorted YFP-expressing nucleus

tag: genotype

tag: treatment

value: AiP13968 PHP.eB


title: C57Bl/6 YFP+ ALM single nucleus DLX2.0-H2B-SYFP2-BC-34

description:
Library name: AB-S40324_E1-50_GTAGTGGATT-CTTCTTAGGC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus DLX2.0-H2B-SYFP2-BC-30

description:
Library name: AB-S40324_E1-50_CAACTGAAGC-GTACGTATGG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus DLX2.0-H2B-SYFP2-BC-28

description:
Library name: AB-S40324_E1-50_GCTAACCGTG-CCGTCATATA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus DLX2.0-H2B-SYFP2-BC-27

description:
Library name: AB-S40324_E1-50_TCAAGCACGG-TTCCGCCGAT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus DLX2.0-H2B-SYFP2-BC-26

description:
Library name: AB-S40324_E1-50_CCGACCTGAA-ACATGCGTAA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus DLX2.0-H2B-SYFP2-BC-29

description:
Library name: AB-S40324_E1-50_AGAACGATCT-CCATTAGCTT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus CMV-H2B-SYFP2-BC-38

description:
Library name: AB-S40324_E1-50_GTTGGACATA-ATCCTCTGTT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.

value: AiP13967 PHP.eB


title: C57Bl/6 YFP+ ALM single nucleus CMV-H2B-SYFP2-BC-37

description:
Library name: AB-S40324_E1-50_CGCTTCACAG-CTTCACCTAA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus CMV-H2B-SYFP2-BC-40

description:
Library name: AB-S40324_E1-50_GAGGTAGTTC-ACTAAGGCAG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus CMV-H2B-SYFP2-BC-35

description:
Library name: AB-S40324_E1-50_CATGTAAGGT-GTAGCAATTG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus CMV-H2B-SYFP2-BC-33

description:
Library name: AB-S40324_E1-50_TCTCTTAGGT-ACATCTGCTT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus CMV-H2B-SYFP2-BC-34

description:
Library name: AB-S40324_E1-50_CGTCACCTTC-TCTAGAGGTG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0391h-H2B-SYFP2-BC-22

description:
Library name: AB-S40324_E1-50_CCTGTACTAA-GAGGCTCGTT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.

value: AiP14058 PHP.eB


title: C57Bl/6 YFP+ ALM single nucleus CMV-H2B-SYFP2-BC-36

description:
Library name: AB-S40324_E1-50_TTATTCACCG-CCTATAGTTG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0391h-H2B-SYFP2-BC-24

description:
Library name: AB-S40324_E1-50_ATAACAGGTC-AGATCTATGG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0391h-H2B-SYFP2-BC-19

description:
Library name: AB-S40324_E1-50_AATGACAAGG-CTCATCAACA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus DLX2.0-H2B-SYFP2-BC-25

description:
Library name: AB-S40324_E1-50_CACTAGTTAC-GTTAGGAATG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0391h-H2B-SYFP2-BC-23

description:
Library name: AB-S40324_E1-50_ACCACACAAT-AACTCATGTC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus CMV-H2B-SYFP2-BC-39

description:
Library name: AB-S40324_E1-50_AATCACCTCT-CGTGTACTGC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0391h-H2B-SYFP2-BC-17

description:
Library name: AB-S40324_E1-50_CATTCTCTGG-GTTGTGTACG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0391h-H2B-SYFP2-BC-18

description:
Library name: AB-S40324_E1-50_ACGGAACTGC-TGACTCTACG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0391h-H2B-SYFP2-BC-15

description:
Library name: AB-S40324_E1-50_TTCCTACGAG-TGATCGCACG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0391h-H2B-SYFP2-BC-14

description:
Library name: AB-S40324_E1-50_CGAGCACACA-GTTAGGTGTT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0391h-H2B-SYFP2-BC-12

description:
Library name: AB-S40324_E1-50_GTAAGTCGCG-CTTACGTACG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0391h-H2B-SYFP2-BC-11

description:
Library name: AB-S40324_E1-50_AGTGAAGCGC-CTGATAAGAC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0391h-H2B-SYFP2-BC-10

description:
Library name: AB-S40324_E1-50_ATCCAGCCGA-AAGCTAGCGG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0391h-H2B-SYFP2-BC-20

description:
Library name: AB-S40324_E1-50_AGGTGCCTTA-ACGTGCGTTC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0391h-H2B-SYFP2-BC-21

description:
Library name: AB-S40324_E1-50_GAATAGTGCT-CTATGTTACG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0391h-H2B-SYFP2-BC-9

description:
Library name: AB-S40324_E1-50_TTGGCCGAAT-TGGCTGTCGT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0391h-H2B-SYFP2-BC-16

description:
Library name: AB-S40324_E1-50_TGCAGCAGCA-AGCTCAGTGG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0391h-H2B-SYFP2-BC-7

description:
Library name: AB-S40324_E1-50_TGATTGCGTA-GAGCAGACGA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0391h-H2B-SYFP2-BC-6

description:
Library name: AB-S40324_E1-50_CTTGCTTACA-AAGGTTGGCT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0391h-H2B-SYFP2-BC-8

description:
Library name: AB-S40324_E1-50_GCATAGTTAG-CCACGAGTTA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0391h-H2B-SYFP2-BC-5

description:
Library name: AB-S40324_E1-50_GATTGCTAGG-ACGTGACGTG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0391h-H2B-SYFP2-BC-13

description:
Library name: AB-S40324_E1-50_ACAGCCTGTC-AGTTCAGCAC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0391h-H2B-SYFP2-BC-2

description:
Library name: AB-S40324_E1-50_GTCGGTTCAC-ACACCTGTAT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0391h-H2B-SYFP2-BC-4

description:
Library name: AB-S40324_E1-50_CTACGGTATG-CCTCCTCTTG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0401h-H2B-SYFP2-BC-24

description:
Library name: AB-S40324_E1-50_CACCGGTCTA-ATCCTAGCCT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.

value: AiP14057 PHP.eB


title: C57Bl/6 YFP+ ALM single nucleus AiE0401h-H2B-SYFP2-BC-23

description:
Library name: AB-S40324_E1-50_ACTGGCCGAA-TGCAAGGTTA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0401h-H2B-SYFP2-BC-21

description:
Library name: AB-S40324_E1-50_GTAAGCACAG-AGTAATGCCT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0391h-H2B-SYFP2-BC-1

description:
Library name: AB-S40324_E1-50_TCCTAGTATC-ATAGCTTGTG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0401h-H2B-SYFP2-BC-17

description:
Library name: AB-S40324_E1-50_CCACTGTGAC-TGGACAGATG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0401h-H2B-SYFP2-BC-18

description:
Library name: AB-S40324_E1-50_GTGTACGTCG-TCCTAGCAGG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0401h-H2B-SYFP2-BC-19

description:
Library name: AB-S40324_E1-50_TCACCGTCAC-CAACTCCTTA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0401h-H2B-SYFP2-BC-22

description:
Library name: AB-S40324_E1-50_GAGCTCCACA-CAGAACAGAA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0401h-H2B-SYFP2-BC-15

description:
Library name: AB-S40324_E1-50_ACACCTACAT-CTGACGTCGT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0391h-H2B-SYFP2-BC-3

description:
Library name: AB-S40324_E1-50_GCCTCACTAG-TTATAGCTGG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0401h-H2B-SYFP2-BC-20

description:
Library name: AB-S40324_E1-50_GAATGTCCAA-GAATACCGTA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0401h-H2B-SYFP2-BC-16

description:
Library name: AB-S40324_E1-50_AGAAGGAACA-AGACGTTGAA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0401h-H2B-SYFP2-BC-12

description:
Library name: AB-S40324_E1-50_GAGAGCTAAC-CAATACTTGC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0401h-H2B-SYFP2-BC-14

description:
Library name: AB-S40324_E1-50_CAACCGATTA-TGCTGAGTTA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0401h-H2B-SYFP2-BC-8

description:
Library name: AB-S40324_E1-50_TTCAGAGGTC-CTGGAGCATC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0401h-H2B-SYFP2-BC-11

description:
Library name: AB-S40324_E1-50_GCTGCCGATA-TATCCTACGT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0401h-H2B-SYFP2-BC-13

description:
Library name: AB-S40324_E1-50_AATCTCAGGC-TCTCTAACTC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0401h-H2B-SYFP2-BC-10

description:
Library name: AB-S40324_E1-50_AGAGACTTAC-TAAGAGTTGG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0401h-H2B-SYFP2-BC-7

description:
Library name: AB-S40324_E1-50_TCAGTAGCAG-TTGGTTATCG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0401h-H2B-SYFP2-BC-6

description:
Library name: AB-S40324_E1-50_TTATCTGTGC-CACTCAGTAT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0401h-H2B-SYFP2-BC-2

description:
Library name: AB-S40324_E1-50_TTAGGAGGAA-GAACTTGCGT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0401h-H2B-SYFP2-BC-5

description:
Library name: AB-S40324_E1-50_TCACCAGGAC-TCCTTGTCTC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0387m-H2B-SYFP2-BC-21

description:
Library name: AB-S40324_E1-50_TTGGTGCATT-GAATCCAGGA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.

value: AiP14056 PHP.eB


title: C57Bl/6 YFP+ ALM single nucleus AiE0401h-H2B-SYFP2-BC-4

description:
Library name: AB-S40324_E1-50_AGGTGGCTAC-CCACGTAACG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0387m-H2B-SYFP2-BC-18

description:
Library name: AB-S40324_E1-50_GCTGCTAACA-AGCTCTTGTG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0401h-H2B-SYFP2-BC-1

description:
Library name: AB-S40324_E1-50_ATTGCGGCTG-TGTCCTACAA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0401h-H2B-SYFP2-BC-9

description:
Library name: AB-S40324_E1-50_ATGGAGACGG-CTGAGAGTTC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0401h-H2B-SYFP2-BC-3

description:
Library name: AB-S40324_E1-50_ACAGAAGGTT-TGGAGATATC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0387m-H2B-SYFP2-BC-24

description:
Library name: AB-S40324_E1-50_GTACACTAGT-ACCGAGATCA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0387m-H2B-SYFP2-BC-14

description:
Library name: AB-S40324_E1-50_CGCTTAAGTA-ATTACTGCAC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0387m-H2B-SYFP2-BC-10

description:
Library name: AB-S40324_E1-50_TATGGTGATG-TTCAAGTAGC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0387m-H2B-SYFP2-BC-15

description:
Library name: AB-S40324_E1-50_ATGCCAGAAT-ACCAAGCAGC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0387m-H2B-SYFP2-BC-16

description:
Library name: AB-S40324_E1-50_AAGTTAGGAC-GCAGCTGTGA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0387m-H2B-SYFP2-BC-12

description:
Library name: AB-S40324_E1-50_ACCGACGTGA-AACGGTCATG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0387m-H2B-SYFP2-BC-9

description:
Library name: AB-S40324_E1-50_ACTGGTGTCG-CAAGTCCTGT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0387m-H2B-SYFP2-BC-20

description:
Library name: AB-S40324_E1-50_GTTGGACGGT-AGGACACTGT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0387m-H2B-SYFP2-BC-22

description:
Library name: AB-S40324_E1-50_CACGGTTGGT-CTAGCTGAAT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0387m-H2B-SYFP2-BC-23

description:
Library name: AB-S40324_E1-50_CACATCACCA-GATTAACTGG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0387m-H2B-SYFP2-BC-19

description:
Library name: AB-S40324_E1-50_AGCTATCTTC-GTGTCGCTAG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0387m-H2B-SYFP2-BC-7

description:
Library name: AB-S40324_E1-50_AAGCTAGCTG-GCACTGACGA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0387m-H2B-SYFP2-BC-8

description:
Library name: AB-S40324_E1-50_CTCAAGGCTC-TAAGAACACG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0387m-H2B-SYFP2-BC-13

description:
Library name: AB-S40324_E1-50_GCATACTCTA-TAGCGAGATA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0387m-H2B-SYFP2-BC-17

description:
Library name: AB-S40324_E1-50_TATCGCGACA-CTAGTGACCT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0387m-H2B-SYFP2-BC-11

description:
Library name: AB-S40324_E1-50_ACCAGACGGT-CGTCTTCATC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0387m-H2B-SYFP2-BC-6

description:
Library name: AB-S40324_E1-50_GTGTTATCTC-ACACCAGACT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0410m-H2B-SYFP2-BC-40

description:
Library name: AB-S40324_E1-50_CTCTGGAGCT-AGTTGACGAA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.

value: AiP12820 PHP.eB


title: C57Bl/6 YFP+ ALM single nucleus AiE0387m-H2B-SYFP2-BC-5

description:
Library name: AB-S40324_E1-50_CTCTCATGCG-ACGTCGGTTG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0387m-H2B-SYFP2-BC-3

description:
Library name: AB-S40324_E1-50_AGATCTTCCA-GTGTTGATCT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0410m-H2B-SYFP2-BC-39

description:
Library name: AB-S40324_E1-50_AGGTAAGATG-AACGTGACTA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0387m-H2B-SYFP2-BC-2

description:
Library name: AB-S40324_E1-50_AACAACCTCA-TTGGACTAAG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0387m-H2B-SYFP2-BC-4

description:
Library name: AB-S40324_E1-50_CGTAGATCGC-GTGAACGCGT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0387m-H2B-SYFP2-BC-1

description:
Library name: AB-S40324_E1-50_TATCACTCTC-ACCTTGTTCT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0410m-H2B-SYFP2-BC-38

description:
Library name: AB-S40324_E1-50_CTATACACCA-CCAACTACGG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0410m-H2B-SYFP2-BC-35

description:
Library name: AB-S40324_E1-50_AATGGTACTG-ACAGCAGTTA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0410m-H2B-SYFP2-BC-33

description:
Library name: AB-S40324_E1-50_CGATAGCAGG-TCGTAGCATT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0390m-H2B-SYFP2-BC-40

description:
Library name: AB-S40324_E1-50_ACGCTCATAA-ACTATTGGTG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.

value: AiP12818 PHP.eB


title: C57Bl/6 YFP+ ALM single nucleus AiE0410m-H2B-SYFP2-BC-37

description:
Library name: AB-S40324_E1-50_TTCCACCTGG-ATGAGCTTCT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0390m-H2B-SYFP2-BC-39

description:
Library name: AB-S40324_E1-50_AACCGCATGC-TCCTTCCAGC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0390m-H2B-SYFP2-BC-38

description:
Library name: AB-S40324_E1-50_TTCAGGTCGT-TTGGTTGGTT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0410m-H2B-SYFP2-BC-34

description:
Library name: AB-S40324_E1-50_TCGGTCTTGC-GTTAAGTTGG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0390m-H2B-SYFP2-BC-35

description:
Library name: AB-S40324_E1-50_GTTCCACCGA-CCTGCTGTTA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0410m-H2B-SYFP2-BC-36

description:
Library name: AB-S40324_E1-50_CAGCCTTGAG-TGAGTAGCAT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0390m-H2B-SYFP2-BC-36

description:
Library name: AB-S40324_E1-50_GACGTTCTCT-AGTAGAGCTA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0390m-H2B-SYFP2-BC-34

description:
Library name: AB-S40324_E1-50_GAGCTTATGA-ACCAGTCATT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0475m-H2B-SYFP2-BC-6

description:
Library name: AB-S40324_E1-50_ACTTGACTAG-GTATCAACGG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.

value: AiP14062 PHP.eB


title: C57Bl/6 YFP+ ALM single nucleus AiE0475m-H2B-SYFP2-BC-4

description:
Library name: AB-S40324_E1-50_TATAACTCCG-TATGTGTCCT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0390m-H2B-SYFP2-BC-33

description:
Library name: AB-S40324_E1-50_GATGAAGGAT-GAATAAGGCT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0390m-H2B-SYFP2-BC-37

description:
Library name: AB-S40324_E1-50_GCGCTTCTAC-GTCCTTGGCT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0475m-H2B-SYFP2-BC-7

description:
Library name: AB-S40324_E1-50_ACAACCAACT-ACAAGATGGT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0475m-H2B-SYFP2-BC-8

description:
Library name: AB-S40324_E1-50_GCAACCGAAC-CGAAGAACAG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0475m-H2B-SYFP2-BC-5

description:
Library name: AB-S40324_E1-50_AACACAACGA-ACGCAGCCAA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0475m-H2B-SYFP2-BC-2

description:
Library name: AB-S40324_E1-50_CCAAGACACT-ACCGACATGG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0140h-H2B-SYFP2-BC-6

description:
Library name: AB-S40324_E1-50_AGAGGAATCG-CCAGACTATC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.

value: AiP14061 PHP.eB


title: C57Bl/6 YFP+ ALM single nucleus AiE0475m-H2B-SYFP2-BC-1

description:
Library name: AB-S40324_E1-50_GCTGAGAGCT-GTGATAGGTG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0140h-H2B-SYFP2-BC-7

description:
Library name: AB-S40324_E1-50_ATGGTTGACT-TGGCACAAGC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0475m-H2B-SYFP2-BC-3

description:
Library name: AB-S40324_E1-50_TGGATATGGC-TGGCGCGTAT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0140h-H2B-SYFP2-BC-1

description:
Library name: AB-S40324_E1-50_CGCAGAACTT-TATCGACGTC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0140h-H2B-SYFP2-BC-4

description:
Library name: AB-S40324_E1-50_ATCTTCCTAG-TGCGCGTGTA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0078h-H2B-SYFP2-BC-6

description:
Library name: AB-S40324_E1-50_GCAGTCTTAT-TAATGGCCGG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.

value: AiP14060 PHP.eB


title: C57Bl/6 YFP+ ALM single nucleus AiE0078h-H2B-SYFP2-BC-8

description:
Library name: AB-S40324_E1-50_CTGCCTCATC-CTATGGCAGG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0140h-H2B-SYFP2-BC-2

description:
Library name: AB-S40324_E1-50_CAGAACGTGG-GACTTCACCG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0140h-H2B-SYFP2-BC-5

description:
Library name: AB-S40324_E1-50_TTATCGCTGA-TTAGGATTGG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0140h-H2B-SYFP2-BC-3

description:
Library name: AB-S40324_E1-50_GCCTAGGACT-GAGAACGGCT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0078h-H2B-SYFP2-BC-7

description:
Library name: AB-S40324_E1-50_CCTCCAGAAG-GTGTTCCAAT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0078h-H2B-SYFP2-BC-4

description:
Library name: AB-S40324_E1-50_TATCGTTGTG-TGTGTCCGCA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus DLX2.0-H2B-SYFP2-BC-24

description:
Library name: AB-S40324_E1-50_AGACGTGTTA-CCAACTGTAA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0140h-H2B-SYFP2-BC-8

description:
Library name: AB-S40324_E1-50_CTCACTTGGC-CGATCGCTGT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0078h-H2B-SYFP2-BC-1

description:
Library name: AB-S40324_E1-50_CTGCGTACTC-CTGATGGCAA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0078h-H2B-SYFP2-BC-2

description:
Library name: AB-S40324_E1-50_GCCTTACTTA-GCACCTTAAC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0078h-H2B-SYFP2-BC-3

description:
Library name: AB-S40324_E1-50_CGGAACGAAG-ACCATCGCCT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0078h-H2B-SYFP2-BC-5

description:
Library name: AB-S40324_E1-50_AACTCGACAT-GTGGTTGAAG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus DLX2.0-H2B-SYFP2-BC-22

description:
Library name: AB-S40324_E1-50_GTCAACGTAA-TGAGTTGACT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus DLX2.0-H2B-SYFP2-BC-21

description:
Library name: AB-S40324_E1-50_CATAGTGTCT-TAATGCACAG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus DLX2.0-H2B-SYFP2-BC-20

description:
Library name: AB-S40324_E1-50_ATGAGGCTGC-CTGCTCCTCT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus DLX2.0-H2B-SYFP2-BC-17

description:
Library name: AB-S40324_E1-50_ATCGGTTCCA-CTTGGTGGAG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus DLX2.0-H2B-SYFP2-BC-16

description:
Library name: AB-S40324_E1-50_CATTCGATTC-TGAGACACTC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus DLX2.0-H2B-SYFP2-BC-23

description:
Library name: AB-S40324_E1-50_AATACGAGGT-GCTGCGCTAT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus DLX2.0-H2B-SYFP2-BC-19

description:
Library name: AB-S40324_E1-50_CCACATCATA-TTGTGCTTGG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus DLX2.0-H2B-SYFP2-BC-15

description:
Library name: AB-S40324_E1-50_CGTCGAGCAT-CAGTGTCATC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus DLX2.0-H2B-SYFP2-BC-18

description:
Library name: AB-S40324_E1-50_ATAGCTCTAG-CGCTGTAGGA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus DLX2.0-H2B-SYFP2-BC-14

description:
Library name: AB-S40324_E1-50_ATTCTTGCCT-GTAGCTGCAC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus DLX2.0-H2B-SYFP2-BC-13

description:
Library name: AB-S40324_E1-50_TTAACCTGCG-ACATCAGTAG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus DLX2.0-H2B-SYFP2-BC-11

description:
Library name: AB-S40324_E1-50_CTGCAGATCG-GTGGACTGTC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus DLX2.0-H2B-SYFP2-BC-10

description:
Library name: AB-S40324_E1-50_CTACGTACGG-GTGCTAGATC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus CMV-H2B-SYFP2-BC-32

description:
Library name: AB-S40324_E1-50_ACACCGCTTA-TGCCTAGTAA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus DLX2.0-H2B-SYFP2-BC-9

description:
Library name: AB-S40324_E1-50_AGCACAGCAT-CTATCGCGCT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus DLX2.0-H2B-SYFP2-BC-12

description:
Library name: AB-S40324_E1-50_GCCTTAACGA-GATGTCGGTC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus CMV-H2B-SYFP2-BC-31

description:
Library name: AB-S40324_E1-50_GCAGTTAGAC-TCAATGCGTC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus CMV-H2B-SYFP2-BC-29

description:
Library name: AB-S40324_E1-50_CTTGGTATCA-AGCCTATACA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus CMV-H2B-SYFP2-BC-30

description:
Library name: AB-S40324_E1-50_CAGCACTACG-TTCTGTGGCG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus CMV-H2B-SYFP2-BC-26

description:
Library name: AB-S40324_E1-50_AGCGAAGCTG-CACGACAATT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus CMV-H2B-SYFP2-BC-25

description:
Library name: AB-S40324_E1-50_ACCTTGTGTC-TGCGGTCCTT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus CMV-H2B-SYFP2-BC-27

description:
Library name: AB-S40324_E1-50_GTTGGAATCT-TGACCGCTTC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus CMV-H2B-SYFP2-BC-24

description:
Library name: AB-S40324_E1-50_TAGCCTCATG-ACCGGAAGGT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus CMV-H2B-SYFP2-BC-23

description:
Library name: AB-S40324_E1-50_AGTTGCATCT-ACCTGCCAAT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus CMV-H2B-SYFP2-BC-21

description:
Library name: AB-S40324_E1-50_CTTGGAGCGA-GAGAACCATA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus CMV-H2B-SYFP2-BC-22

description:
Library name: AB-S40324_E1-50_CTGTCGTACA-ACTGCGAATG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus CMV-H2B-SYFP2-BC-20

description:
Library name: AB-S40324_E1-50_TGTCTGCCAT-TCAAGATGCG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus CMV-H2B-SYFP2-BC-19

description:
Library name: AB-S40324_E1-50_AGCACCTATT-GCGATAGGCA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus CMV-H2B-SYFP2-BC-17

description:
Library name: AB-S40324_E1-50_ATCACAGTCT-GAACGTTGTT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus CMV-H2B-SYFP2-BC-16

description:
Library name: AB-S40324_E1-50_CGGCTAACCA-CTACCACTAT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus CMV-H2B-SYFP2-BC-18

description:
Library name: AB-S40324_E1-50_CCGTAACCAC-CAATGGTGGA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus CMV-H2B-SYFP2-BC-15

description:
Library name: AB-S40324_E1-50_TCCTCTCGAA-ACATGACTGA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus CMV-H2B-SYFP2-BC-10

description:
Library name: AB-S40324_E1-50_GTTGCGTGCT-CCTCATCGTT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus CMV-H2B-SYFP2-BC-11

description:
Library name: AB-S40324_E1-50_GCATTCTCGC-TTCGCGCCTA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus CMV-H2B-SYFP2-BC-13

description:
Library name: AB-S40324_E1-50_TGCTCATTCA-ACGTCATCGG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus CMV-H2B-SYFP2-BC-28

description:
Library name: AB-S40324_E1-50_ATACGATCAC-ACATCGGTGT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus CMV-H2B-SYFP2-BC-12

description:
Library name: AB-S40324_E1-50_CGCAATTCTA-ATAGGTTGGA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0410m-H2B-SYFP2-BC-31

description:
Library name: AB-S40324_E1-50_GCGGTTGATA-GCTGTATCTT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0410m-H2B-SYFP2-BC-30

description:
Library name: AB-S40324_E1-50_TACCACGGCT-AGATCCAGCA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus CMV-H2B-SYFP2-BC-14

description:
Library name: AB-S40324_E1-50_TCTAGGAAGT-TTACGTCCTA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0410m-H2B-SYFP2-BC-29

description:
Library name: AB-S40324_E1-50_AAGAGAGAAG-GTCAGCTCGT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0410m-H2B-SYFP2-BC-26

description:
Library name: AB-S40324_E1-50_CTGGACCATC-CGATGTTGAT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0410m-H2B-SYFP2-BC-24

description:
Library name: AB-S40324_E1-50_TGCTCCTAAC-CGGCTAGGAA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0410m-H2B-SYFP2-BC-27

description:
Library name: AB-S40324_E1-50_AACACGGTGA-TAACCGCCAC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0410m-H2B-SYFP2-BC-32

description:
Library name: AB-S40324_E1-50_AACATCTCGA-GTCTTGCAGG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0410m-H2B-SYFP2-BC-28

description:
Library name: AB-S40324_E1-50_AATCCATTGC-TCCGATGGTT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0410m-H2B-SYFP2-BC-23

description:
Library name: AB-S40324_E1-50_ATCTCGCATT-AAGAAGATGC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0410m-H2B-SYFP2-BC-22

description:
Library name: AB-S40324_E1-50_TCGAGCGTTA-TGTCGTGCTT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0410m-H2B-SYFP2-BC-19

description:
Library name: AB-S40324_E1-50_GAGGAATTAC-TGGCAGTGTC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus CMV-H2B-SYFP2-BC-9

description:
Library name: AB-S40324_E1-50_TGTCTTCATC-ATTCACGCTA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0410m-H2B-SYFP2-BC-16

description:
Library name: AB-S40324_E1-50_AGCATGTAAC-TAGTTCAGCT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0410m-H2B-SYFP2-BC-20

description:
Library name: AB-S40324_E1-50_GACATAACCT-ATCCTAACAG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0410m-H2B-SYFP2-BC-21

description:
Library name: AB-S40324_E1-50_GCAATGATGC-CTCCGTTGAG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0410m-H2B-SYFP2-BC-17

description:
Library name: AB-S40324_E1-50_TTCGGAATGA-GTAAGGTAGT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0410m-H2B-SYFP2-BC-18

description:
Library name: AB-S40324_E1-50_CCTTGCCGTA-CCATAGGAGT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0410m-H2B-SYFP2-BC-15

description:
Library name: AB-S40324_E1-50_ATAGGTCGTC-GATATGTGCA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0410m-H2B-SYFP2-BC-14

description:
Library name: AB-S40324_E1-50_AATAGCCATC-AACGTCGTTA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0410m-H2B-SYFP2-BC-25

description:
Library name: AB-S40324_E1-50_CAACAGTAAC-CAATCCTAGA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0410m-H2B-SYFP2-BC-13

description:
Library name: AB-S40324_E1-50_CAAGAGGCTT-TTGCTACGCT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0410m-H2B-SYFP2-BC-12

description:
Library name: AB-S40324_E1-50_CTTCTCAGAG-TGACGCTTAG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0410m-H2B-SYFP2-BC-11

description:
Library name: AB-S40324_E1-50_TAGGCCTCCT-GTGGTCAATT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0410m-H2B-SYFP2-BC-10

description:
Library name: AB-S40324_E1-50_ACATACTAGC-TGGAACCACG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0410m-H2B-SYFP2-BC-9

description:
Library name: AB-S40324_E1-50_CCAACCGTTC-CATCTCTTGC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0390m-H2B-SYFP2-BC-31

description:
Library name: AB-S40324_E1-50_ACTTGAAGTG-CACCACATTA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0390m-H2B-SYFP2-BC-29

description:
Library name: AB-S40324_E1-50_ACTGTCGACG-TTGTAGGAAG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0390m-H2B-SYFP2-BC-32

description:
Library name: AB-S40324_E1-50_TGCTCGTGAG-GTGGCGTGTT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0390m-H2B-SYFP2-BC-28

description:
Library name: AB-S40324_E1-50_GCCAGGTTCT-AGCGCACATA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0390m-H2B-SYFP2-BC-30

description:
Library name: AB-S40324_E1-50_CTAGCAGTTA-CCAGCTCTGT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0390m-H2B-SYFP2-BC-27

description:
Library name: AB-S40324_E1-50_TCAATGAGGT-ACGTACAAGA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0390m-H2B-SYFP2-BC-25

description:
Library name: AB-S40324_E1-50_CGTGATTGGT-CGGTATATGC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0390m-H2B-SYFP2-BC-21

description:
Library name: AB-S40324_E1-50_CGACGTACAC-TTATCGACCT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0390m-H2B-SYFP2-BC-24

description:
Library name: AB-S40324_E1-50_GTGAATGGTT-TTGTAGAGCT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0390m-H2B-SYFP2-BC-20

description:
Library name: AB-S40324_E1-50_AACAAGTGAG-AGTTACGCAT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0390m-H2B-SYFP2-BC-19

description:
Library name: AB-S40324_E1-50_GACCATCTAC-TCGTCACTGG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0390m-H2B-SYFP2-BC-22

description:
Library name: AB-S40324_E1-50_AGCGTGACCT-ACTCGGATGG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0390m-H2B-SYFP2-BC-23

description:
Library name: AB-S40324_E1-50_GCACTTACAA-AACCAGTTAG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0390m-H2B-SYFP2-BC-15

description:
Library name: AB-S40324_E1-50_AATGGACCTT-TCGTTGGATA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0390m-H2B-SYFP2-BC-26

description:
Library name: AB-S40324_E1-50_CGGAGATACG-CAGACCTACG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0390m-H2B-SYFP2-BC-14

description:
Library name: AB-S40324_E1-50_TTGGCACTTA-TACGACGCAC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0390m-H2B-SYFP2-BC-17

description:
Library name: AB-S40324_E1-50_TAGCTTCGTA-ACGTTGAGGT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0390m-H2B-SYFP2-BC-11

description:
Library name: AB-S40324_E1-50_TCAACGCGTA-TTAGCATCGA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0390m-H2B-SYFP2-BC-13

description:
Library name: AB-S40324_E1-50_ACGGAGAGGT-GTCAGTAGCT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0390m-H2B-SYFP2-BC-18

description:
Library name: AB-S40324_E1-50_ACCAAGACTC-AGTGTTCGTG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0390m-H2B-SYFP2-BC-16

description:
Library name: AB-S40324_E1-50_GCCGTCATAA-CTGCACTCAC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0390m-H2B-SYFP2-BC-12

description:
Library name: AB-S40324_E1-50_ACAACGAATC-GACTGTACGG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0390m-H2B-SYFP2-BC-9

description:
Library name: AB-S40324_E1-50_CCGGATCCAA-GCAGTGAAGT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0390m-H2B-SYFP2-BC-10

description:
Library name: AB-S40324_E1-50_CGTACGCATT-ACGCGCAACA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus hSyn1-H2B-SYFP2-BC-38

description:
Library name: AB-S40323_E1-50_CAACCATGAT-ATAAGGTGAC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.

value: AiP13648 PHP.eB


title: C57Bl/6 YFP+ ALM single nucleus hSyn1-H2B-SYFP2-BC-36

description:
Library name: AB-S40323_E1-50_GATAGGTGAA-AGACGCAAGA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus hSyn1-H2B-SYFP2-BC-35

description:
Library name: AB-S40323_E1-50_ATAGCCGGTT-TCTTATCCGG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus hSyn1-H2B-SYFP2-BC-39

description:
Library name: AB-S40323_E1-50_TACCTAGACT-GCATTCGAAT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus hSyn1-H2B-SYFP2-BC-37

description:
Library name: AB-S40323_E1-50_AAGGCTCCAT-TGAAGATGGA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus hSyn1-H2B-SYFP2-BC-34

description:
Library name: AB-S40323_E1-50_GCACTACCAG-GAAGACTTAG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus hSyn1-H2B-SYFP2-BC-40

description:
Library name: AB-S40323_E1-50_CGTCGCCTAT-TATCTTCTCG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus hSyn1-H2B-SYFP2-BC-33

description:
Library name: AB-S40323_E1-50_ATCCAGCATG-AGAGCTCTAC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus hSyn1-H2B-SYFP2-BC-32

description:
Library name: AB-S40323_E1-50_CACTAGACTT-TGTGGATTAG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus hSyn1-H2B-SYFP2-BC-30

description:
Library name: AB-S40323_E1-50_TCTACAGCTA-AATGGAAGAC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus hSyn1-H2B-SYFP2-BC-29

description:
Library name: AB-S40323_E1-50_CTCTCTCTAG-ACTTGATTGG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus hSyn1-H2B-SYFP2-BC-31

description:
Library name: AB-S40323_E1-50_AGCACCAGTC-CTCGTGTAGC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus hSyn1-H2B-SYFP2-BC-28

description:
Library name: AB-S40323_E1-50_TAACCTGAAC-TCAATTGACG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus hSyn1-H2B-SYFP2-BC-27

description:
Library name: AB-S40323_E1-50_CTCTATACTG-CGTTCAACCG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus hSyn1-H2B-SYFP2-BC-25

description:
Library name: AB-S40323_E1-50_AGAAGATTGG-AGAAGGTACT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus hSyn1-H2B-SYFP2-BC-26

description:
Library name: AB-S40323_E1-50_CATATGGTGC-ACAGATCTCA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus hSyn1-H2B-SYFP2-BC-24

description:
Library name: AB-S40323_E1-50_CTGAACGTAT-GCCAATACAT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus hSyn1-H2B-SYFP2-BC-23

description:
Library name: AB-S40323_E1-50_TACCGGAGCA-CTTAGCAGGC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus hSyn1-H2B-SYFP2-BC-20

description:
Library name: AB-S40323_E1-50_TAGGTCCAGA-TGCTCATCCG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus hSyn1-H2B-SYFP2-BC-21

description:
Library name: AB-S40323_E1-50_CGGATCATTA-CCGTAACCTT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus hSyn1-H2B-SYFP2-BC-22

description:
Library name: AB-S40323_E1-50_TGTCGGTGCT-AGTTCTTCCG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus hSyn1-H2B-SYFP2-BC-18

description:
Library name: AB-S40323_E1-50_TGGCACTGAT-GCCATAGAAT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus hSyn1-H2B-SYFP2-BC-16

description:
Library name: AB-S40323_E1-50_TGTGATCGCG-AGACGAGTCT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus hSyn1-H2B-SYFP2-BC-19

description:
Library name: AB-S40323_E1-50_AGCGTCTCAT-CCTAGGCGAT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus hSyn1-H2B-SYFP2-BC-13

description:
Library name: AB-S40323_E1-50_ATACACACCT-AGTGTGATAG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus hSyn1-H2B-SYFP2-BC-11

description:
Library name: AB-S40323_E1-50_TGGAAGCACA-TCCAACACCG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus hSyn1-H2B-SYFP2-BC-12

description:
Library name: AB-S40323_E1-50_TCAGCTGTCA-ACAGCTCCTG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus hSyn1-H2B-SYFP2-BC-14

description:
Library name: AB-S40323_E1-50_AGCCACTCGT-CCAAGAGGAT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus hSyn1-H2B-SYFP2-BC-10

description:
Library name: AB-S40323_E1-50_ACCAGGAATT-AGCATGGCAT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus hSyn1-H2B-SYFP2-BC-9

description:
Library name: AB-S40323_E1-50_CTGTATGCCA-TTCCGGCTGT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus DLX2.0-H2B-SYFP2-BC-7

description:
Library name: AB-S40323_E1-50_AATACGAGGT-GCTGCGCTAT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus DLX2.0-H2B-SYFP2-BC-5

description:
Library name: AB-S40323_E1-50_CATAGTGTCT-TAATGCACAG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus DLX2.0-H2B-SYFP2-BC-8

description:
Library name: AB-S40323_E1-50_AGACGTGTTA-CCAACTGTAA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus hSyn1-H2B-SYFP2-BC-15

description:
Library name: AB-S40323_E1-50_GCATTCCTTC-GAGCCTGCTT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus hSyn1-H2B-SYFP2-BC-17

description:
Library name: AB-S40323_E1-50_TGTGACGTAT-GCAGCGTTAG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus DLX2.0-H2B-SYFP2-BC-6

description:
Library name: AB-S40323_E1-50_GTCAACGTAA-TGAGTTGACT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus DLX2.0-H2B-SYFP2-BC-4

description:
Library name: AB-S40323_E1-50_ATGAGGCTGC-CTGCTCCTCT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus CMV-H2B-SYFP2-BC-7

description:
Library name: AB-S40323_E1-50_GAAGGAGCCT-ATAATGGAGG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus CMV-H2B-SYFP2-BC-6

description:
Library name: AB-S40323_E1-50_GTAGATCCGG-TTGTTCGGAG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus DLX2.0-H2B-SYFP2-BC-2

description:
Library name: AB-S40323_E1-50_ATAGCTCTAG-CGCTGTAGGA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus CMV-H2B-SYFP2-BC-8

description:
Library name: AB-S40323_E1-50_TTCAAGGAGA-CGATCATTCG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus CMV-H2B-SYFP2-BC-4

description:
Library name: AB-S40323_E1-50_CTTCCAGTTG-ACTCATAGTG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus DLX2.0-H2B-SYFP2-BC-1

description:
Library name: AB-S40323_E1-50_ATCGGTTCCA-CTTGGTGGAG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus CMV-H2B-SYFP2-BC-5

description:
Library name: AB-S40323_E1-50_ATGATACCTG-TATGAGACTC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus CMV-H2B-SYFP2-BC-3

description:
Library name: AB-S40323_E1-50_AACCGTTATG-TAGAGTGTCT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus CMV-H2B-SYFP2-BC-1

description:
Library name: AB-S40323_E1-50_TAGGACAGCT-GTGATTGTTC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus CMV-H2B-SYFP2-BC-2

description:
Library name: AB-S40323_E1-50_AAGCGGTGAA-AACCTAGACG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0410m-H2B-SYFP2-BC-8

description:
Library name: AB-S40323_E1-50_CATTCGATTC-TGAGACACTC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus DLX2.0-H2B-SYFP2-BC-3

description:
Library name: AB-S40323_E1-50_CCACATCATA-TTGTGCTTGG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0410m-H2B-SYFP2-BC-4

description:
Library name: AB-S40323_E1-50_GCCTTAACGA-GATGTCGGTC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0410m-H2B-SYFP2-BC-5

description:
Library name: AB-S40323_E1-50_TTAACCTGCG-ACATCAGTAG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0410m-H2B-SYFP2-BC-7

description:
Library name: AB-S40323_E1-50_CGTCGAGCAT-CAGTGTCATC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0410m-H2B-SYFP2-BC-6

description:
Library name: AB-S40323_E1-50_ATTCTTGCCT-GTAGCTGCAC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0410m-H2B-SYFP2-BC-2

description:
Library name: AB-S40323_E1-50_CTACGTACGG-GTGCTAGATC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0410m-H2B-SYFP2-BC-1

description:
Library name: AB-S40323_E1-50_AGCACAGCAT-CTATCGCGCT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0410m-H2B-SYFP2-BC-3

description:
Library name: AB-S40323_E1-50_CTGCAGATCG-GTGGACTGTC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0390m-H2B-SYFP2-BC-8

description:
Library name: AB-S40323_E1-50_ACACCGCTTA-TGCCTAGTAA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0390m-H2B-SYFP2-BC-5

description:
Library name: AB-S40323_E1-50_CTTGGTATCA-AGCCTATACA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0390m-H2B-SYFP2-BC-6

description:
Library name: AB-S40323_E1-50_CAGCACTACG-TTCTGTGGCG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0390m-H2B-SYFP2-BC-4

description:
Library name: AB-S40323_E1-50_ATACGATCAC-ACATCGGTGT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0390m-H2B-SYFP2-BC-2

description:
Library name: AB-S40323_E1-50_AGCGAAGCTG-CACGACAATT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0390m-H2B-SYFP2-BC-1

description:
Library name: AB-S40323_E1-50_ACCTTGTGTC-TGCGGTCCTT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus hSyn1-H2B-SYFP2-BC-7

description:
Library name: AB-S40323_E1-50_AGTTGCATCT-ACCTGCCAAT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus hSyn1-H2B-SYFP2-BC-8

description:
Library name: AB-S40323_E1-50_TAGCCTCATG-ACCGGAAGGT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus hSyn1-H2B-SYFP2-BC-2

description:
Library name: AB-S40323_E1-50_CCGTAACCAC-CAATGGTGGA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus AiE0390m-H2B-SYFP2-BC-7

description:
Library name: AB-S40323_E1-50_GCAGTTAGAC-TCAATGCGTC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus hSyn1-H2B-SYFP2-BC-5

description:
Library name: AB-S40323_E1-50_CTTGGAGCGA-GAGAACCATA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: C57Bl/6 YFP+ ALM single nucleus hSyn1-H2B-SYFP2-BC-4

description:
Library name: AB-S40323_E1-50_TGTCTGCCAT-TCAAGATGCG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: CD-1 mouse brain, amygdala, sub stimulus

city: Austin

first: Madeleine

last: Dwortz

state: TX

address: 2506 Speedway, NMS BLDG

department: Center for Biomedical Research Support

institute: University of Texas at Austin

description:
Library name: Sample Sub
10x Genomics

data_processing:
Sequencing data were processed using Cell Ranger v7.1.0 (10x Genomics) on the 10x Genomics Cloud Analysis platform. Reads were aligned to the mm10 reference genome and cell-calling and gene-by-nucleus count matrices were generated using default pipeline settings.
Assembly: Mouse mm 10
Supplementary files format and content: GRCmm39

molecule: nuclear RNA

source_name: brain

extract_protocol:
Mouse amygdala tissue was microdissected and pooled by condition. Tissue was homogenized in Nuclei EZ Lysis Buffer with RNase inhibitor using a chilled Dounce homogenizer. The lysate was filtered through a 35 µm strainer and centrifuged. Nuclei were purified using a 25%/29% iodixanol gradient, washed in 2% BSA/PBS with RNase inhibitor, filtered again, stained with Trypan Blue, and counted using an automated cell counter.
Single-nucleus suspensions were loaded onto the Chromium Controller and libraries were prepared using the Chromium Next GEM Single Cell 3′ Kit v3.1 (10x Genomics) following the manufacturer’s protocol, targeting ~10,000 nuclei per library. Libraries were sequenced on an Illumina NovaSeq 6000 using an S1 flow cell with standard read configuration.

treatment_protocol: Social olfactory stimulus

value: brain

value: subordinate stimulus


title: CD-1 mouse brain, amygdala, dom stimulus

description:
Library name: Sample Dom
10x Genomics

value: dominant stimulus


title: Cerebral tissue， sham, rep1

city: Wuhu

first: Min

last: Peng

address: Zheshan West Road num2

country: China

institute: First Affiliated Hospital of Wannan Medical College (Yijishan Hospital)

description:
Library name: Sample 1
10x Genomics

data_processing:
Sequencing results were demultiplexed and converted to FASTQ format using Illumina bcl2fastq software (version 5.0.1). Sample demultiplexing, barcode processing, and single-cell 3’gene counting was performed with Cell Ranger (version 7.2.0), and scRNA-seq data were aligned to the Ensembl genome GRCm39 reference genome. The pipelines were run with the assistance of LC-Bio Technology.
We performed downstream analysis using the Seurat package (version 4.4.0). Cells with fewer than 500 detected genes and cells with mitochondrial transcript percentages exceeding 25% were excluded from the analysis. Additionally, we used the DoubletFinder software (version 2.0.4) to remove potential doublets. After filtering out low-quality cells and doublets, the data were normalized using the NormalizeData function, high-variable genes were identified with the FindVariableFeatures function, and the data were scaled using the ScaleData function. Subsequently, dimensionality reduction was performed using RunPCA for principal component analysis. Clustering was conducted using the FindNeighbors and FindClusters functions. Finally, the clustering results were visualized using DimPlot.During the dimensionality reduction and clustering process, we used the harmony function to correct for batch effects (when necessary; batch correction was not performed for samples from the same batch). The FindAllMarkers function was utilized to identify characteristic genes for each cluster. Subsequently, we performed enrichment analysis on the characteristic genes of each cluster using the clusterProfiler R package (version 4.12.6) with default settings. The mouse annotation information was sourced from the org.Mm.eg.db database.
Assembly: Ensembl_v101
Supplementary files format and content: barcodes.tsv.gz: Contains the cell barcodes that passed Cell Ranger's quality filters. Each barcode represents a single cell.Text file (GZIP compressed), one barcode per line.
Supplementary files format and content: features.tsv.gz: Contains the identifiers for all quantified genes (features).Text file (GZIP compressed). Typically has columns for gene ID and gene symbol.
Supplementary files format and content: matrix.mtx.gz: Contains the gene expression counts (UMI counts) in a sparse matrix format.Sparse matrix file (GZIP compressed). Stores row index (gene), column index (cell), and count value.

source_name: Cerebral tissue

growth_protocol: mice were housed under controlled conditions with a constant temperature (26 ± 2 °C), relative humidity (50 ± 10%), and a 12-hour light/dark cycle. All animals had ad libitum access to food and water.

extract_protocol:
Transfer fresh Cerebral tissue into a culture dish (the dish is placed on moist ice), wash the tissue surface with 1X PBS to remove bloodstains, lipids, and other adhering substances. Cut the tissue into 0.5mm(2) pieces, transfer the tissue into a 15mL centrifuge tube, add 5mL enzyme solution (1mg/mL sigma collagenase I, 1mg/mL sigma collagenase II, 0.5mg/mL sigma neutral protease), react at 37°C in a water bath shaker (100rpm) for 30 minutes, then add 1× PBS (containing 10% fetal bovine serum FBS) to terminate digestion, and repeatedly pipette the mixture 5-10 times with a transfer pipette. Subsequently, filter through a 40μm cell strainer, collect the cell suspension in a 15mL centrifuge tube, centrifuge at 4°C, 500g for 50 minutes. After centrifugation, discard the supernatant, add 3 mL red blood cell lysis solution (MACS 130-094-183, 10×), incubate at room temperature for 5 minutes. After lysis, centrifuge at 4°C, 300g for 5 minutes, collect the cell pellet after centrifugation, remove dead cells using a Dead Cell Removal Kit (MACS 130-090-101), resuspend the cells in 1× PBS (0.04% BSA), and adjust the cell concentration to 700-1200 cells per μL.
According to the 10x Genomics Chromium Single Cell 3' Reagent Kits (V3.1) manual, load the single-cell suspension onto a 10x Chromium chip, expecting to capture 8000 cells. Perform cDNA amplification and library construction following the standard protocol. The libraries are sequenced on an Illumina NovaSeq 6000 sequencing system (paired-end sequencing, 150bp) by LC-Bio Technology Company (Hangzhou, China).

treatment_protocol: IL-25 at a dose of 1 µg per mouse within 30 minutes after TBI, followed by daily injections for three consecutive days

value: Cerebral tissue

tag: age

value: 6-8 weeks old

tag: Sex

value: male


title: C57Bl/6 YFP+ ALM single nucleus AiE0390m-H2B-SYFP2-BC-3

description:
Library name: AB-S40323_E1-50_GTTGGAATCT-TGACCGCTTC
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.

value: anterior lateral motor cortex nucleus

value: sorted YFP-expressing nucleus


title: Motolimod-treated

city: Shanghai

first: Qikai

last: Zhou

state: Shanghai

address: 1954 Huashan Road, Xuhui District, Shanghai

institute: Shanghai Jiao Tong University

description: Library name: Sample 2

data_processing:
The CellRanger (v 5.0.1) was used to align the clean reads with mm10. The Seura 79 (v 5.1.0) pipeline was integrated into analysis and visualization, including clustering, dimension reduction, and cell type identification. Cell types were annotated based on the expression of known markers shown in Figure S3-E. The genes expressed in less than three cells were removed. The cells that expressed less than 200 genes were not included in the analysis. The harmony was used to remove the batch effects between samples. The dimension reduction of umap was calculated based on 40 harmony components. The significantly up-regulated genes in the specific sample and/or subpopulation were identified by FindMarkers and FindAllMarkers. The clusterProfiler was used to annotate the top markers of each subpopulation with the KEGG database. The macrophage gene score was evaluated with AddModuleScore. The ggplot2 was used to display the expression patterns of gene modules.
Assembly: Mouse mm10
Supplementary files format and content: Tab-separated values files and matrix files

molecule: total RNA

source_name: Tumor

extract_protocol:
To control the final number of cells captured, approximately 30,000 cells per sample were loaded onto 10X Chromium Single Cell Platform (10X Genomics) at a concentration of 1,000 cells per μl (Single Cell 3′ library and Gel Bead Kit v.3) as described in the manufacturer’s protocol
Library was performed according to the manufacter's instructions (single cell 3’v3protocol, 10x Genomics). Briefly, GCs were resuspended in the master mix and loadedtogether with partitioning oil and gel beads into the chip to generate the gel bead-in-emulsion (GEM). The poly-A RNA from the cell lysate contained in every single GEMwas retrotranscripted to cDNA, which contains an llumina R1 primer sequence, UniqueMolecular ldentifier (UMl) and the 10x Barcode. The pooled barcoded cDNA was thencleaned up with Silane DynaBeads, amplified by PCR and the apropiated sizedfragments were selected with SPRlselect reagent for subsequent library construction.During the library construction llumina R2 primer sequence, paired-end constructs withP5 and P7 sequences and a sample index were added.

value: Tumor

value: Motolimod-treated


title: KM, PBMC, control

city: Changsha

first: Nouhoum

last: Dibo

state: Hunan

address: No. South Lushan Road

institute: Central South University

description:
Library name: Sample 3
Singleron

data_processing:
Cutadapt v1.17 was used to remove low-quality reads, trim poly-A tails, and adapter sequences. Raw reads from scRNA-seq were processed to generate a gene expression matrix using CeleScope (https://github.com/singleron-RD/CeleScope) v1.9.0 pipeline.
Assembly: mouse reference genome GRCm38 (Ensembl version 92 annotation)
Supplementary files format and content: Sparse Matrix Market(MTX), Gene Count Matrix

source_name: Blood

extract_protocol:
The Isolated cells were washed with Ca/Mg-free PBS before adding 2 mL GEXSCOPE® red blood cell lysis buﬀer (RCLB, Singleron) at 25°C for 10 minutes to remove the red blood cells (RBCs). After removing the RBCs, the cells were washed with PBS, and the PBMCs were isolated by centrifugation. The PBMCs were stained with Trypan Blue, and the cell viability was evaluated microscopically.
Single-cell suspensions were loaded onto a microwell chip using the Singleron Matrix® Single Cell Processing System. Barcoding Beads were subsequently collected from the microwell chip, followed by reverse transcription of the mRNA and PCR amplification. The amplified cDNA is then fragmented and ligated with sequencing adapters. The scRNA-seq libraries were constructed according to the protocol of the GEXSCOPE® Single Cell RNA Library Kits (Singleron). Individual libraries were diluted to 4 nM, pooled, and sequenced on Illumina Novaseq 6000 with 150 bp paired-end reads.

value: Blood

tag: cell line

value: Mus musculus

value: Peripheral blood mononuclear cells

value: uninfected


title: Control

description: Library name: Sample 1

value: Control


title: Cerebral tissue，PBS treatment for three days after TBI, rep1

description:
Library name: Sample 2
10x Genomics


title: Cerebral tissue，IL-25 treatment for three days after TBI, rep1

description:
Library name: Sample 3
10x Genomics


title: iWAT MondoAKO Chow Diet 7

city: Philadelphia

first: Daniel

last: Kelly

state: PA

address: 3400 Civic Center Blvd

department: Penn Cardiovascular Institute

institute: University of Pennsylvania

description: Library name: iWAT7

library_source: transcriptomic

data_processing:
Reads in each known mm10 transcript were quantified using Salmon 1.10.3
Assembly: mm10
Supplementary files format and content: Salmon quantification files, tab separated

source_name: iWAT

extract_protocol:
RNA isolation and qRT-PCR. Total RNA was isolated from frozen tissues or cells using the RNeasy Mini Kit (QIAGEN) and treated with RNase-Free DNase Set (QIAGEN) per manufacturer’s instructions. cDNA was synthesized using the Affinity Script cDNA Synthesis Kit (Agilent Technologies) or High-Capacity cDNA Reverse Transcription Kit (ThermoFisher Scientific #4368814) with 0.25 μg total RNA.
RNA-Seq library preparation, sequencing and analysis. GENEWIZ, LLC. (South Plainfield, NJ) performed RNA library preparations and sequencing reactions per standard operating procedure [16, 17].

treatment_protocol: standard chow (Lab Diet 5010) or a 60% fat HFD (Research Diets, D12492i)

value: iWAT

value: MondoAKO

value: Chow Diet


title: iWAT MondoAKO Chow Diet 9

description: Library name: iWAT9


title: C57Bl/6 YFP+ ALM single nucleus hSyn1-H2B-SYFP2-BC-6

description:
Library name: AB-S40323_E1-50_CTGTCGTACA-ACTGCGAATG
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.

value: AiP13648 PHP.eB


title: C57Bl/6 YFP+ ALM single nucleus hSyn1-H2B-SYFP2-BC-3

description:
Library name: AB-S40323_E1-50_AGCACCTATT-GCGATAGGCA
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: KM, PBMC, infected

description:
Library name: Sample 4
Singleron

value: S. japonicum


title: iWAT WT Chow Diet 2

description: Library name: iWAT2

value: WT


title: iWAT MondoAKO Chow Diet 8

description: Library name: iWAT8


title: iWAT WT Chow Diet 5

description: Library name: iWAT5


title: iWAT WT Chow Diet 6

description: Library name: iWAT6


title: C57Bl/6 YFP+ ALM single nucleus hSyn1-H2B-SYFP2-BC-1

description:
Library name: AB-S40323_E1-50_ATCACAGTCT-GAACGTTGTT
SSv4-profiled YFP-expressing single flow-sorted nucleus.  Sequenced to a depth of approx 0.5M read pairs.


title: iWAT MondoAKO High Fat Diet 22

description: Library name: iWAT22

value: High Fat Diet


title: iWAT WT Chow Diet 1

description: Library name: iWAT1


title: iWAT WT Chow Diet 4

description: Library name: iWAT4


title: iWAT MondoAKO High Fat Diet 23

description: Library name: iWAT23


title: iWAT WT Chow Diet 3

description: Library name: iWAT3


title: iWAT MondoAKO High Fat Diet 24

description: Library name: iWAT24


title: iWAT MondoAKO High Fat Diet 20

description: Library name: iWAT20


title: iWAT WT High Fat Diet 18

description: Library name: iWAT18


title: iWAT WT High Fat Diet 16

description: Library name: iWAT16


title: iWAT MondoAKO High Fat Diet 21

description: Library name: iWAT21


title: iWAT WT High Fat Diet 13

description: Library name: iWAT13


title: iWAT MondoAKO Chow Diet 12

description: Library name: iWAT12


title: iWAT MondoAKO Chow Diet 11

description: Library name: iWAT11


title: iWAT MondoAKO Chow Diet 10

description: Library name: iWAT10


title: iWAT MondoAKO High Fat Diet 19

description: Library name: iWAT19


title: iWAT WT High Fat Diet 14

description: Library name: iWAT14


title: RNA-seq of Atf4 exon2 cKO mice  E11.5  heart replicate 4

city: San Diego

first: Zeyu

last: Chen

state: CA

address: 9500 Gilman Drive, BFRII 1217

department: School of Medicine

institute: UCSD

description:
Library name: Atf4_e2_cKO_rep4
Atf4.Batch2.e2_cKO4

data_processing:
Raw RNA-seq reads were assessed for quality using FastQC.
Adapter sequences and low-quality bases were trimmed using Trim Galore (v0.6.7).
Clean reads were aligned to the mouse reference genome (GRCm38, Mus musculus) using HISAT2 (v2.2.1) with default parameters.
Gene expression quantification was performed using StringTie (v2.2.1).
Gene counts were obtained with featureCounts and used for differential expression analysis with DESeq2.
Assembly: GRCm38 (Mus musculus)
Supplementary files format and content: normalized bigWig files (*.bw) representing RNA-seq read coverage for each sample
Supplementary files format and content: a text file (merge.TPM.txt) containing  TPM expression values across all samples.

source_name: Ventricular heart

growth_protocol: All mice were housed in a pathogen-free facility under standard laboratory conditions with a 12-hour light/dark cycle. Mice were maintained on a standard rodent chow diet with water provided ad libitum. Timed matings were set up, and noon on the day of vaginal plug detection was considered embryonic day 0.5 (E0.5). Pregnant dams were euthanized at E11.5 to collect embryonic heart tissues.

extract_protocol:
Total RNA was extracted from cultured primary cortical neurons using the Quick-RNA Miniprep Kit (Zymo Research; Cat# R1054) following the manufacturer's instructions. RNA concentration and purity were assessed using a Nanodrop spectrophotometer. For downstream applications, equal amounts of RNA were used for either RNA-seq library preparation or reverse transcription into cDNA using the High-Capacity cDNA Reverse Transcription Kit (Invitrogen™).
RNA-seq libraries were constructed following ribosomal RNA (rRNA) depletion protocols to enrich for non-rRNA transcripts. Total RNA was subjected to rRNA depletion using a commercial RNA depletion kit according to the manufacturer’s instructions. Libraries were then generated from the rRNA-depleted RNA and sequenced on an Illumina NovaSeq 6000 platform using paired-end sequencing.

treatment_protocol: No chemical or pharmacological treatments were applied in this study. All embryos were harvested under baseline physiological conditions without experimental perturbation.

value: Ventricular heart

value: C57BL/6 WT

value: ATF4 exon2 cKO

tag: batch


title: iWAT WT High Fat Diet 15

description: Library name: iWAT15


title: RNA-seq of Atf4 exon2 Control mice  E11.5  heart replicate 1

description:
Library name: Atf4_e2_Ctrl_rep1
Atf4.Batch2.CT1


title: RNA-seq of Atf4 exon2-3 cKO mice  E11.5  heart replicate 2

description:
Library name: Atf4_e2e3_cKO_rep2
Atf4.Batch1.e23_cKO2

value: ATF4 exon2-3 cKO


title: RNA-seq of Atf4 exon2 Control mice  E11.5  heart replicate 3

description:
Library name: Atf4_e2_Ctrl_rep3
Atf4.Batch2.CT3


title: RNA-seq of Atf4 exon2-3 Control mice  E11.5  heart replicate 2

description:
Library name: Atf4_e2e3_Ctrl_rep2
Atf4.Batch1.CT2


title: RNA-seq of Atf4 exon2-3 cKO mice  E11.5  heart replicate 3

description:
Library name: Atf4_e2e3_cKO_rep3
Atf4.Batch1.e23_cKO3


title: iWAT WT High Fat Diet 17

description: Library name: iWAT17


title: RNA-seq of Atf4 exon2-3 cKO mice  E11.5  heart replicate 1

description:
Library name: Atf4_e2e3_cKO_rep1
Atf4.Batch1.e23_cKO1


title: RNA-seq of Atf4 exon2 cKO mice  E11.5  heart replicate 2

description:
Library name: Atf4_e2_cKO_rep2
Atf4.Batch2.e2_cKO2


title: RNA-seq of Control mice E11.5 heart replicate 2

description:
Library name: Atf4.7del.CT_rep2
control

data_processing:
Raw RNA-seq reads were assessed for quality using FastQC.
Adapter sequences and low-quality bases were trimmed using Trim Galore (v0.6.7).
Clean reads were aligned to the mouse reference genome (GRCm38, Mus musculus) using HISAT2 (v2.2.1) with default parameters.
Gene expression quantification was performed using StringTie (v2.2.1).
Gene counts were obtained with featureCounts and used for differential expression analysis with DESeq2.
Assembly: GRCm38 (Mus musculus)
Supplementary files format and content: normalized bigWig files (*.bw) representing RNA-seq read coverage for each sample
Supplementary files format and content: BAM files were subsetted to the genomic region surrounding the Atf4 locus (chr15:79,481,845–81,029,726, mm10) to visualize splicing junctions and transcript structures within this interval.
Supplementary files format and content: a text file (merge.TPM.txt) containing  TPM expression values across all samples.


title: RNA-seq of Control mice E11.5 heart replicate 4

description:
Library name: Atf4.7del.CT_rep4
control


title: RNA-seq of Atf4 frameshift mutant mice E11.5 heart replicate 2

description:
Library name: Atf4.7del.MT_rep2
ATF4 global knockout

value: ATF4 global knockout


title: RNA-seq of  Control mice  E11.5  heart replicate 4

description:
Library name: CT_rep4
control

data_processing:
Raw RNA-seq reads were assessed for quality using FastQC.
Adapter sequences and low-quality bases were trimmed using Trim Galore (v0.6.7).
Clean reads were aligned to the mouse reference genome (GRCm38, Mus musculus) using HISAT2 (v2.2.1) with default parameters.
Gene expression quantification was performed using StringTie (v2.2.1).
Gene counts were obtained with featureCounts and used for differential expression analysis with DESeq2. Differentially expressed genes (DEGs) were identified using the criteria: |Fold change| ≥ 1.2 and q < 0.05.
Assembly: GRCm38 (Mus musculus)
Supplementary files format and content: normalized bigWig files (*.bw) representing RNA-seq read coverage for each sample
Supplementary files format and content: a text file (merge.TPM.txt) containing  TPM expression values across all samples.

extract_protocol:
Total RNA was extracted from cultured primary cortical neurons using the Quick-RNA Miniprep Kit (Zymo Research; Cat# R1054) following the manufacturer's instructions. RNA concentration and purity were assessed using a Nanodrop spectrophotometer. For downstream applications, equal amounts of RNA were used for either RNA-seq library preparation or reverse transcription into cDNA using the High-Capacity cDNA Reverse Transcription Kit (Invitrogen™). Gene expression validation was performed by quantitative PCR (qPCR) using the Applied Biosystems 7300 Real-Time PCR System, and analyzed with the ΔΔCt method.
RNA-seq libraries were constructed following ribosomal RNA (rRNA) depletion protocols to enrich for non-rRNA transcripts. Total RNA was subjected to rRNA depletion using a commercial RNA depletion kit according to the manufacturer’s instructions. Libraries were then generated from the rRNA-depleted RNA and sequenced on an Illumina NovaSeq 6000 platform using paired-end sequencing.


title: RNA-seq of Control mice E11.5 heart replicate 1

description:
Library name: Atf4.7del.CT_rep1
control


title: RNA-seq of Atf4 exon2-3 Control mice  E11.5  heart replicate 4

description:
Library name: Atf4_e2e3_Ctrl_rep4
Atf4.Batch1.CT4


title: RNA-seq of Atf4 exon2 cKO mice  E11.5  heart replicate 1

description:
Library name: Atf4_e2_cKO_rep1
Atf4.Batch2.e2_cKO1


title: RNA-seq of Atf4 exon2-3 Control mice  E11.5  heart replicate 1

description:
Library name: Atf4_e2e3_Ctrl_rep1
Atf4.Batch1.CT1


title: RNA-seq of Atf4 exon2 Control mice  E11.5  heart replicate 4

description:
Library name: Atf4_e2_Ctrl_rep4
Atf4.Batch2.CT4


title: RNA-seq of  Control mice  E11.5  heart replicate 3

description:
Library name: CT_rep3
control


title: RNA-seq of Atf4 exon2-3 Control mice  E11.5  heart replicate 3

description:
Library name: Atf4_e2e3_Ctrl_rep3
Atf4.Batch1.CT3


title: RNA-seq of Atf4 exon2 cKO mice  E11.5  heart replicate 3

description:
Library name: Atf4_e2_cKO_rep3
Atf4.Batch2.e2_cKO3


title: RNA-seq of  Control mice  E11.5  heart replicate 2

description:
Library name: CT_rep2
control


title: RNA-seq of Atf4 frameshift mutant mice E11.5 heart replicate 1

description:
Library name: Atf4.7del.MT_rep1
ATF4 global knockout


title: RNA-seq of Atf4 frameshift mutant mice E11.5 heart replicate 4

description:
Library name: Atf4.7del.MT_rep4
ATF4 global knockout


title: RNA-seq of Atf4 frameshift mutant mice E11.5 heart replicate 3

description:
Library name: Atf4.7del.MT_rep3
ATF4 global knockout


title: RNA-seq of Atf4 exon2-3 cKO mice  E11.5  heart replicate 4

description:
Library name: Atf4_e2e3_cKO_rep4
Atf4.Batch1.e23_cKO4


title: RNA-seq of Control mice E11.5 heart replicate 3

description:
Library name: Atf4.7del.CT_rep3
control


title: RNA-seq of  Control mice  E11.5  heart replicate 1

description:
Library name: CT_rep1
control


title: RNA-seq of  CM-specific Rps19bp1 KO mice  E11.5  heart replicate 4

description:
Library name: Rps19bp1_cKO_rep4
conditional KO

value: CM-specific Rps19bp1 KO


title: RNA-seq of  CM-specific Rps19bp1 KO mice  E11.5  heart replicate 2

description:
Library name: Rps19bp1_cKO_rep2
conditional KO


title: RNA-seq of  CM-specific Rps19bp1 KO mice  E11.5  heart replicate 3

description:
Library name: Rps19bp1_cKO_rep3
conditional KO


title: RNA-seq of  CM-specific Rps19bp1 KO mice  E11.5  heart replicate 1

description:
Library name: Rps19bp1_cKO_rep1
conditional KO


title: ConA+SIM-2

city: Beijing

first: Xiyu

last: Wang

address: No. 8 Gongren Tiyuchang South Road

institute: Beijing Chaoyang Hospital, Capital Medical University

description: Library name: Sample 5

data_processing:
HISAT2 2.1.0 --dta –t –p 4
RNA samples were purified, fragmented, reverse transcribed, end-repaired. After sequenced adapters were added, the target fragments were recovered by magnetic bead screening, and PCR amplification was performed to complete the entire library preparation work.
After the library construction was completed, use Agilent2100 to detect the insert size of the library.
If insert size met the expectation, Bio-RAD CFX 96 fluorescent quantitative PCR instrument and Bio-RAD KIT iQ SYBR GRN were used to perform q-PCR to accurately quantify the effective concentration of the library (the effective concentration of the library was > 10nM) to ensure the quality of the library.
Assembly: Mus_musculus.GRCm38.91
Supplementary files format and content: tab-delimited text files include raw counts for each Sample

source_name: liver

extract_protocol:
Mice were performed oral gavage with a 30 mg/kg body weight mixed solution of simvastatin or PBS per day before 5 days of ConA injection and 1 h before the injection. An additional oral gavage was administered to the mice 24 h after ConA injection.. CD4+ T cells in liver samples were stained and detected by fluorescence activated cell sorting (FACS) cells Aria II (BD Biosciences, CA, USA), CD3+ NK1.1-CD4+ CD8- cells were selected.
RNA libraries were prepared for sequencing using standard Illumina protocols.

value: liver

value: Primary cells

value: T cell

value: Wild type


title: ConA+SIM-3

description: Library name: Sample 6


title: ConA-2

description: Library name: Sample 2


title: ConA+SIM-1

description: Library name: Sample 4


title: TCR-seq, B16-F10 melanoma cells, Mettl5 KO, rep4

city: Shenzhen

first: Hao

address: 1088 Xueyuan Avenue

institute: Southern University of Science and Technology (SUSTech)

description: Library name: TCRseq-C21

data_processing:
Processed reads were aligned to the reference genome (gencode GRCm39) by the STAR (v2.7.10a)80, allowing for at most two mismatches. For Ribo-seq data, quality control and translational profiles was analyzed by RiboseQC. Transcriptome assembly and quantification were performed using StringTie (v2.1.7)81. For TCR-seq, data were analysed using ImmunoSEQ Analyzer 3.0, an Adaptive Biotechnologies online analysis platform. For scRNA-seq, Single-cell RNA sequencing (scRNA-seq) data were processed using standardized bioinformatics workflows. Raw sequencing data were aligned to the Mouse reference genome (GRCm39) and converted into unique molecular identifier (UMI) count matrices using Cell Ranger.
Assembly: GRCm39.genome.fa
Library strategy: TCR-Seq

source_name: B16-F10 melanoma cells

growth_protocol: The B16-F10 melanoma cells were cultured in Dulbecco's Modified Eagle Medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS, Biological Industries), 1% penicillin-streptomycin (Thermo Fisher Scientific), and 1% SaveIt mycoplasma inhibitor (Hanbio Biotechnology). Cells were maintained in a humidified incubator at 37°C with 5% CO₂ and routinely tested for bacterial and mycoplasma contamination.

extract_protocol:
For Ribo-Seq, cells were incubated with 100 μg/ml Cycloheximide (MCE) for 8 min at 37°C and were then lysed on ice for 10min in 400uL lysis buffer (20 mM Tris–HCl pH 7.5, 150 mM NaCl, 5 mM MgCl2, 1 mM DTT, 100 μg/ml Cycloheximide, 1% Triton X-100). The supernatant was collected via centrifugation at 12 000 g for 10 min at 4 °C, and its' absorbance at 260nm was measured. The ribosomes with equal A260 in each group were reserved for RNA sequencing. For TCR-Seq, PBMCs were isolated from whole blood of C57BL/6N mice that had been inoculated with either control B16F10 cells or Mettl5-KO cells 12 days prior.   For scRNA-Seq,  Tumors were surgically resected and preserved in MACS Tissue Storage Solution, then transferred to the laboratory at low temperature. Following the manufacturer's instructions for the Miltenyi Biotec dissociation kit, the tumors were dissociated into a single-cell suspension. Post-dissociation, cells were resuspended in MACS Tissue Storage Solution to maintain viability. Cell counting and concentration adjustment were performed, followed by labeling with Zombie NIR viability dye to distinguish live and dead cells. Subsequently, cells were incubated with Fc blocker in FACS buffer to prevent non-specific antibody binding. Cells were then incubated with an antibody cocktail containing anti-CD45 antibodies. After incubation, cells were washed, filtered through a 70-μm Macs SmartStrainer to remove aggregates and debris, ensuring a high-quality single-cell suspension. Finally, CD45+ live immune cells were sorted using a BD FACSAria II instrument for downstream scRNA-seq analysis with the 10X Genomics 5' platform. This method ensures the isolation of viable immune cells, crucial for studying the tumor immune microenvironment.
For Ribo-Seq, added 15U RNase1 (Thermo Fisher Scientific) for each 1OD of lysate, and incubated the reactions at 22°C for 40 min with gentle mixing. Ribosome-protected fragments (RPFs) were collected by MicroSpin S-400 columns (Cytiva) and purified by Zymo Research RNA Clean & Concentrator-25 kit (Zymo). The RPFs were then separated with 15% polyacrylamide TBE–urea gel, excised the gel slices between 17 and 34 nucleotides and recovered RNA from PAGE gel by ZR small-RNA™ PAGE Recovery Kit (Zymo). Purified RNA was treated with PNK(NEB), and the RNA library was prepared by VAHTSTM Small RNA Library Prep Kit for Illumina®(Vazyme). The library concentration was analyzed using the VAHTSTM Library Quantification Kit for Illumina®, and equimolar pools of libraries were sequenced. For TCR-Seq, DNA was extracted from PBMC using a QIAamp DNA Blood Mini Kit (Qiagen), and the concentration was tested using NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific). The DNA was used as a template for PCR amplification, which was performed to generate the library of TCR and BCR. The step 1 PCR amplification protocol was as follows: 95 °C for 5 min; 95 °C for 30 s, 59 °C for 30 s; 72 °C for 1 min for 30 cycles; and 72 °C for 10 min. The step 2 PCR amplification protocol was as follows: 98 °C for 2 min; 98 °C for 30 s, 65 °C for 30 s; 72 °C for 30 s for 10 cycles; and 72 °C for 5 min. The PCR products were purified, and the barcodes were confirmed. The annealed region of these primers was the CDR3 region, as shown by Multiplex PCR Amplifies. The CDR3 regions were sequenced on Illumina NextSeq500 (MyGenostics).  For scRNA-Seq, Following cell sorting, live CD45+ immune cells were resuspended in PBS + 0.04% BSA at 1,000 cells/μL. Libraries were prepared using the 10x Genomics Chromium Next GEM Single Cell 5' v2 Kit according to manufacturer protocol, targeting 10,000 cells per sample. cDNA amplification and fragmentation were performed before Illumina adapter ligation.

value: B16-F10 melanoma cells

value: Mettl5 KO


title: TCR-seq, B16-F10 melanoma cells, Mettl5 KO, rep1

description: Library name: TCRseq-C7