description: Library name: ENU57452B6G320_B10_neuro2190_S50_R1_001_2367706464


status: Public on Apr 11 2026

type: SRA

city: South San Francisco

first: Mitchell

last: Rezzonico

state: CA

address: 1 DNA Way

country: USA

institute: Genentech

description: Library name: ENU57450B6G32_A2_neuro2190_S49_R1_001_2367706180

library_source: transcriptomic

data_processing:
RNA-seq reads were aligned to the mouse reference genome GRCm38 using GSNAP 74. Only uniquely mapping reads were carried forward to downstream analysis. Sample quality was further assessed on a per-plate basis based on total read counts, percentage of reads mapping to the reference genome, and percentage of reads mapping to exons. Reads predominantly mapped to transcript 3’ ends, as expected from the library preparation method.
Assembly: GRCm38

molecule: total RNA

organism: Mus musculus

source_name: Whole brain

growth_protocol: Founder C57BL/6NCrl males were treated with 3 x 100 mg/kg ENU (Sigma) and mutations bred to homozygosity in three generations. Generation 3 mice were collected usually between 8-12 weeks old, and an ear notch for DNA and brain samples were collected. Typically, 12 siblings from each pedigree were screened.

extract_protocol:
To homogenize the tissue, RNAlater was removed, and a 5 mm stainless steel bead (Qiagen) and 1.6 mL of Qiazol (Qiagen) was added. The tissue was homogenized using a Tissue Lyser II (Qiagen) for at least 30 minutes until a homogenous solution was confirmed. 400 µL of the homogenate solution was transferred into a new tube and allowed to sit for 5 minutes before adding 100 µL of chloroform (Sigma). The homogenate-chloroform mixture was shaken vigorously, allowed to stand for 2 minutes before centrifugation for 5 minutes at 4°C and 1000 x g. The top clear layer was removed and stored at -80 °C. For RNA extraction, 120 µL was transferred into a QIAgen S-block and processed on the QIAcube using the RNeasy 96 QIAcube HT Kit.
cDNA fragment libraries were constructed using the SMART-Seq® v4 3’ DE Kit (Takara Bio) following manufacturer’s recommendations, with minor modifications. Total RNA samples were processed in batches of 1152, distributed across twelve 96-well plates. For each sample, 4 ng total RNA was used as input for cDNA synthesis, then amplified for 8 cycles, with each sample in a row receiving a unique in-line index. Amplified cDNAs within a row were then combined into a 12-plex pool, cleaned up with Ampure XP beads (Beckman Coulter) and quantified using the Qubit HS DNA assay kit (Thermo Fisher Scientific). For each of the 96 cDNA pools generated from 12 plates, 400 pg were carried into Nextera XT library preparations (Illumina Inc.), each assigned with one of the 96 unique combinations of Forward and Reverse HT Index Primers included in the SMART-Seq® v4 3’ DE kit. After Ampure XP clean up and Qubit quantification, the 96 pools were combined in an equimolar manner to generate a 1152-plex Illumina library.  This library was concentrated with Ampure XP beads, quantified with Qubit and assessed on the TapeStation with the High Sensitivity D1000 assay (Agilent Technologies).

treatment_protocol: Mice were euthanized by cervical dislocation, then whole brains were collected. Each cerebellum was removed and the remaining brain matter was transferred to RNAlater and stored at 4C for up to 5 days.

tag: tissue

value: Whole brain

tag: partofsubset

value: FALSE


description: Library name: ENU57450B6G313_A10_neuro2190_S49_R1_001_2367705733


description: Library name: ENU57452B6G310_B6_neuro2190_S50_R1_001_2367705801


description: Library name: ENU57450B6G31_A1_neuro2190_S49_R1_001_2367705816


description: Library name: ENU57450B6G312_A9_neuro2190_S49_R1_001_2367706409


description: Library name: ENU57450B6G311_A12_neuro2190_S49_R1_001_2367706630


description: Library name: ENU57450B6G33_A3_neuro2190_S49_R1_001_2367706580


description: Library name: ENU57450B6G310_A11_neuro2190_S49_R1_001_2367705916


description: Library name: ENU57447B6G38_E7_neuro2193_S77_R1_001_2367705937


description: Library name: ENU57447B6G35_E4_neuro2193_S77_R1_001_2367706367


description: Library name: ENU57447B6G32_E2_neuro2193_S77_R1_001_2367706597


description: Library name: ENU57447B6G39_E3_neuro2193_S77_R1_001_2367706673


description: Library name: ENU57447B6G314_E12_neuro2193_S77_R1_001_2367706631


description: Library name: ENU57447B6G313_E11_neuro2193_S77_R1_001_2367706195


description: Library name: ENU57447B6G310_E8_neuro2193_S77_R1_001_2367706240


description: Library name: ENU57446B6G35_D4_neuro2188_S36_R1_001_2367705576


description: Library name: ENU57446B6G36_D5_neuro2188_S36_R1_001_2367706610


description: Library name: ENU57447B6G36_E5_neuro2193_S77_R1_001_2367706554


description: Library name: ENU57447B6G37_E6_neuro2193_S77_R1_001_2367705697


description: Library name: ENU57447B6G31_E1_neuro2193_S77_R1_001_2367705648


description: Library name: ENU57446B6G32_D2_neuro2188_S36_R1_001_2367706466


description: Library name: ENU57446B6G34_D3_neuro2188_S36_R1_001_2367705996


description: Library name: ENU57444B6G32_H1_neuro2185_S16_R1_001_2367706408


description: Library name: ENU57444B6G310_H4_neuro2185_S16_R1_001_2367705890


description: Library name: ENU57446B6G37_D6_neuro2188_S36_R1_001_2367705943


description: Library name: ENU57447B6G311_E9_neuro2193_S77_R1_001_2367706566


description: Library name: ENU57444B6G37_H9_neuro2185_S16_R1_001_2367705660


description: Library name: ENU57447B6G312_E10_neuro2193_S77_R1_001_2367705797


description: Library name: ENU57444B6G38_H10_neuro2185_S16_R1_001_2367706623


description: Library name: ENU57444B6G36_H8_neuro2185_S16_R1_001_2367706287


description: Library name: ENU57438B6G36_D5_neuro2192_S68_R1_001_2367705552


description: Library name: ENU57444B6G33_H2_neuro2185_S16_R1_001_2367705839


description: Library name: ENU57438B6G39_D9_neuro2192_S68_R1_001_2367705792


description: Library name: ENU57444B6G311_H6_neuro2185_S16_R1_001_2367706146


description: Library name: ENU57446B6G31_D1_neuro2188_S36_R1_001_2367706544


description: Library name: ENU57444B6G34_H3_neuro2185_S16_R1_001_2367705883


description: Library name: ENU57444B6G35_H7_neuro2185_S16_R1_001_2367705695


description: Library name: ENU57438B6G38_D8_neuro2192_S68_R1_001_2367706378


description: Library name: ENU57444B6G312_H11_neuro2185_S16_R1_001_2367706508


description: Library name: ENU57438B6G35_D6_neuro2192_S68_R1_001_2367705668


description: Library name: ENU57444B6G314_H12_neuro2185_S16_R1_001_2367706482


description: Library name: ENU57444B6G31_H5_neuro2185_S16_R1_001_2367705725


description: Library name: ENU57438B6G37_D7_neuro2192_S68_R1_001_2367706567


description: Library name: ENU57438B6G33_D3_neuro2192_S68_R1_001_2367706665


description: Library name: ENU57438B6G32_D2_neuro2192_S68_R1_001_2367706581


description: Library name: ENU57438B6G320_D12_neuro2192_S68_R1_001_2367706444


description: Library name: ENU57438B6G34_D4_neuro2192_S68_R1_001_2367705880


description: Library name: ENU57438B6G31_D1_neuro2192_S68_R1_001_2367706256


description: Library name: ENU57438B6G315_D11_neuro2192_S68_R1_001_2367705977


description: Library name: ENU57438B6G314_D10_neuro2192_S68_R1_001_2367706396


description: Library name: ENU57436B6G36_C6_neuro2190_S51_R1_001_2367705719


description: Library name: ENU57436B6G35_C5_neuro2190_S51_R1_001_2367705751


description: Library name: ENU57436B6G32_C2_neuro2190_S51_R1_001_2367706188


description: Library name: ENU57436B6G34_C4_neuro2190_S51_R1_001_2367706501


description: Library name: ENU57436B6G33_C3_neuro2190_S51_R1_001_2367706406


description: Library name: ENU57433B6G35_B3_neuro2189_S42_R1_001_2367706357


description: Library name: ENU57436B6G311_C11_neuro2190_S51_R1_001_2367706309


description: Library name: ENU57436B6G37_C7_neuro2190_S51_R1_001_2367706397


description: Library name: ENU57436B6G310_C10_neuro2190_S51_R1_001_2367705858


description: Library name: ENU57433B6G37_B7_neuro2189_S42_R1_001_2367706528


description: Library name: ENU57433B6G36_B6_neuro2189_S42_R1_001_2367705690


description: Library name: ENU57436B6G38_C8_neuro2190_S51_R1_001_2367705590


description: Library name: ENU57433B6G34_B5_neuro2189_S42_R1_001_2367705957


description: Library name: ENU57436B6G314_C12_neuro2190_S51_R1_001_2367706353


description: Library name: ENU57433B6G39_B8_neuro2189_S42_R1_001_2367705622


description: Library name: ENU57436B6G39_C9_neuro2190_S51_R1_001_2367706224


description: Library name: ENU57436B6G31_C1_neuro2190_S51_R1_001_2367706469


description: Library name: ENU57433B6G313_B12_neuro2189_S42_R1_001_2367706106


description: Library name: ENU57433B6G32_B2_neuro2189_S42_R1_001_2367705940


description: Library name: ENU57433B6G31_B1_neuro2189_S42_R1_001_2367706356


description: Library name: ENU57433B6G311_B10_neuro2189_S42_R1_001_2367706154


description: Library name: ENU57433B6G33_B4_neuro2189_S42_R1_001_2367706656


description: Library name: ENU57431B6G36_D4_neuro2193_S76_R1_001_2367706418


description: Library name: ENU57433B6G310_B9_neuro2189_S42_R1_001_2367706177


description: Library name: ENU57431B6G33_D1_neuro2193_S76_R1_001_2367706493


description: Library name: ENU57431B6G316_D11_neuro2193_S76_R1_001_2367706265


description: Library name: ENU57431B6G315_D10_neuro2193_S76_R1_001_2367705658


description: Library name: ENU57429B6G38_C5_neuro2193_S75_R1_001_2367705772


description: Library name: ENU57431B6G34_D2_neuro2193_S76_R1_001_2367705810


description: Library name: ENU57429B6G39_C6_neuro2193_S75_R1_001_2367705898


description: Library name: ENU57431B6G35_D3_neuro2193_S76_R1_001_2367706070


description: Library name: ENU57431B6G317_D12_neuro2193_S76_R1_001_2367705793


description: Library name: ENU57433B6G312_B11_neuro2189_S42_R1_001_2367706503


description: Library name: ENU57431B6G32_D7_neuro2193_S76_R1_001_2367705969


description: Library name: ENU57429B6G35_C4_neuro2193_S75_R1_001_2367706392


description: Library name: ENU57429B6G33_C3_neuro2193_S75_R1_001_2367706379


description: Library name: ENU57431B6G310_D8_neuro2193_S76_R1_001_2367706511


description: Library name: ENU57431B6G312_D9_neuro2193_S76_R1_001_2367706267


description: Library name: ENU57431B6G31_D6_neuro2193_S76_R1_001_2367706560


description: Library name: ENU57429B6G32_C2_neuro2193_S75_R1_001_2367706387


description: Library name: ENU57429B6G321_C12_neuro2193_S75_R1_001_2367706318


description: Library name: ENU57429B6G314_C8_neuro2193_S75_R1_001_2367705850


description: Library name: ENU57429B6G312_C9_neuro2193_S75_R1_001_2367705652


description: Library name: ENU57429B6G313_C10_neuro2193_S75_R1_001_2367705589


description: Library name: ENU57429B6G316_C11_neuro2193_S75_R1_001_2367705567


description: Library name: ENU57429B6G310_C7_neuro2193_S75_R1_001_2367706607


description: Library name: ENU57428B6G38_H8_neuro2184_S8_R1_001_2367706107


description: Library name: ENU57429B6G31_C1_neuro2193_S75_R1_001_2367706426


description: Library name: ENU57428B6G39_H9_neuro2184_S8_R1_001_2367706565


description: Library name: ENU57428B6G37_H7_neuro2184_S8_R1_001_2367706262


description: Library name: ENU57428B6G34_H4_neuro2184_S8_R1_001_2367705568


description: Library name: ENU57427B6G39_B6_neuro2188_S34_R1_001_2367705833


description: Library name: ENU57427B6G38_B5_neuro2188_S34_R1_001_2367706432


description: Library name: ENU57428B6G32_H2_neuro2184_S8_R1_001_2367706055


description: Library name: ENU57428B6G31_H1_neuro2184_S8_R1_001_2367706263


description: Library name: ENU57428B6G312_H12_neuro2184_S8_R1_001_2367705555


description: Library name: ENU57428B6G310_H10_neuro2184_S8_R1_001_2367706082


description: Library name: ENU57428B6G33_H3_neuro2184_S8_R1_001_2367706517


description: Library name: ENU57428B6G35_H5_neuro2184_S8_R1_001_2367706583


description: Library name: ENU57427B6G35_B2_neuro2188_S34_R1_001_2367705998


description: Library name: ENU57428B6G311_H11_neuro2184_S8_R1_001_2367706644


description: Library name: ENU57427B6G37_B4_neuro2188_S34_R1_001_2367705845


description: Library name: ENU57427B6G36_B3_neuro2188_S34_R1_001_2367706605


description: Library name: ENU57431B6G311_D5_neuro2193_S76_R1_001_2367706546


description: Library name: ENU57427B6G317_B12_neuro2188_S34_R1_001_2367706090


description: Library name: ENU57427B6G33_B1_neuro2188_S34_R1_001_2367705824


description: Library name: ENU57427B6G315_B10_neuro2188_S34_R1_001_2367706496


description: Library name: ENU57427B6G314_B9_neuro2188_S34_R1_001_2367705838


description: Library name: ENU57427B6G312_B7_neuro2188_S34_R1_001_2367706641


description: Library name: ENU57427B6G313_B8_neuro2188_S34_R1_001_2367706450


description: Library name: ENU57427B6G316_B11_neuro2188_S34_R1_001_2367706220


description: Library name: ENU57425B6G37_E5_neuro2190_S53_R1_001_2367706230


description: Library name: ENU57425B6G34_E4_neuro2190_S53_R1_001_2367706494


description: Library name: ENU57425B6G35_E1_neuro2190_S53_R1_001_2367706365


description: Library name: ENU57425B6G312_E10_neuro2190_S53_R1_001_2367706477


description: Library name: ENU57425B6G31_E2_neuro2190_S53_R1_001_2367705919


description: Library name: ENU57425B6G314_E12_neuro2190_S53_R1_001_2367705679


description: Library name: ENU57425B6G310_E8_neuro2190_S53_R1_001_2367706064


description: Library name: ENU57425B6G311_E9_neuro2190_S53_R1_001_2367705837


description: Library name: ENU57425B6G313_E11_neuro2190_S53_R1_001_2367706311


description: Library name: ENU57424B6G39_G3_neuro2190_S55_R1_001_2367706338


description: Library name: ENU57424B6G37_G2_neuro2190_S55_R1_001_2367706033


description: Library name: ENU57425B6G38_E6_neuro2190_S53_R1_001_2367706564


description: Library name: ENU57424B6G34_G6_neuro2190_S55_R1_001_2367705752


description: Library name: ENU57424B6G33_G5_neuro2190_S55_R1_001_2367706472


description: Library name: ENU57425B6G39_E7_neuro2190_S53_R1_001_2367705771


description: Library name: ENU57424B6G320_G12_neuro2190_S55_R1_001_2367706667


description: Library name: ENU57424B6G31_G1_neuro2190_S55_R1_001_2367706360


description: Library name: ENU57425B6G32_E3_neuro2190_S53_R1_001_2367705804


description: Library name: ENU57424B6G35_G7_neuro2190_S55_R1_001_2367706535


description: Library name: ENU57424B6G316_G11_neuro2190_S55_R1_001_2367705815


description: Library name: ENU57424B6G32_G4_neuro2190_S55_R1_001_2367706376


description: Library name: ENU57424B6G315_G10_neuro2190_S55_R1_001_2367705907


description: Library name: ENU57415B6G37_H6_neuro2188_S40_R1_001_2367705574


description: Library name: ENU57415B6G36_H5_neuro2188_S40_R1_001_2367705655


description: Library name: ENU57424B6G311_G8_neuro2190_S55_R1_001_2367705599


description: Library name: ENU57424B6G314_G9_neuro2190_S55_R1_001_2367706147


description: Library name: ENU57415B6G35_H4_neuro2188_S40_R1_001_2367705566


description: Library name: ENU57415B6G34_H3_neuro2188_S40_R1_001_2367706117


description: Library name: ENU57415B6G324_H12_neuro2188_S40_R1_001_2367706390


description: Library name: ENU57415B6G317_H7_neuro2188_S40_R1_001_2367705651


description: Library name: ENU57415B6G318_H8_neuro2188_S40_R1_001_2367705826


description: Library name: ENU57415B6G319_H9_neuro2188_S40_R1_001_2367705909


description: Library name: ENU57413B6G35_H5_neuro2192_S72_R1_001_2367705836


description: Library name: ENU57415B6G31_H1_neuro2188_S40_R1_001_2367705757


description: Library name: ENU57415B6G323_H11_neuro2188_S40_R1_001_2367706270


description: Library name: ENU57415B6G32_H2_neuro2188_S40_R1_001_2367706244


description: Library name: ENU57415B6G322_H10_neuro2188_S40_R1_001_2367706516


description: Library name: ENU57413B6G39_H7_neuro2192_S72_R1_001_2367705605


description: Library name: ENU57413B6G36_H6_neuro2192_S72_R1_001_2367706186


description: Library name: ENU57413B6G34_H4_neuro2192_S72_R1_001_2367705854


description: Library name: ENU57413B6G320_H12_neuro2192_S72_R1_001_2367706272


description: Library name: ENU57413B6G31_H1_neuro2192_S72_R1_001_2367706670


description: Library name: ENU57428B6G36_H6_neuro2184_S8_R1_001_2367705708


description: Library name: ENU57413B6G319_H11_neuro2192_S72_R1_001_2367706163


description: Library name: ENU57413B6G33_H3_neuro2192_S72_R1_001_2367706058


description: Library name: ENU57413B6G32_H2_neuro2192_S72_R1_001_2367706124


description: Library name: ENU57413B6G318_H10_neuro2192_S72_R1_001_2367706621


description: Library name: ENU57413B6G317_H9_neuro2192_S72_R1_001_2367706349


description: Library name: ENU57405B6G39_G3_neuro2189_S47_R1_001_2367706358


description: Library name: ENU57405B6G37_G1_neuro2189_S47_R1_001_2367706266


description: Library name: ENU57405B6G38_G2_neuro2189_S47_R1_001_2367706457


description: Library name: ENU57405B6G317_G11_neuro2189_S47_R1_001_2367705872


description: Library name: ENU57413B6G316_H8_neuro2192_S72_R1_001_2367706632


description: Library name: ENU57405B6G314_G8_neuro2189_S47_R1_001_2367706221


description: Library name: ENU57405B6G313_G7_neuro2189_S47_R1_001_2367706314


description: Library name: ENU57405B6G315_G9_neuro2189_S47_R1_001_2367705983


description: Library name: ENU57405B6G319_G12_neuro2189_S47_R1_001_2367706449


description: Library name: ENU57393B6G35_F2_neuro2188_S38_R1_001_2367706540


description: Library name: ENU57393B6G32_F5_neuro2188_S38_R1_001_2367706233


description: Library name: ENU57405B6G312_G6_neuro2189_S47_R1_001_2367706380


description: Library name: ENU57393B6G322_F9_neuro2188_S38_R1_001_2367705739


description: Library name: ENU57393B6G37_F7_neuro2188_S38_R1_001_2367706252


description: Library name: ENU57393B6G36_F3_neuro2188_S38_R1_001_2367706400


description: Library name: ENU57405B6G311_G5_neuro2189_S47_R1_001_2367705808


description: Library name: ENU57393B6G31_F1_neuro2188_S38_R1_001_2367705770


description: Library name: ENU57405B6G316_G10_neuro2189_S47_R1_001_2367705610


description: Library name: ENU57393B6G33_F6_neuro2188_S38_R1_001_2367705588


description: Library name: ENU57393B6G316_F11_neuro2188_S38_R1_001_2367706514


description: Library name: ENU57393B6G38_F8_neuro2188_S38_R1_001_2367706250


description: Library name: ENU57393B6G315_F10_neuro2188_S38_R1_001_2367706038


description: Library name: ENU57393B6G317_F12_neuro2188_S38_R1_001_2367706059


description: Library name: ENU57405B6G310_G4_neuro2189_S47_R1_001_2367705582


description: Library name: ENU57393B6G314_F4_neuro2188_S38_R1_001_2367706331


description: Library name: ENU57388B6G39_G9_neuro2185_S15_R1_001_2367706366


description: Library name: ENU57388B6G38_G8_neuro2185_S15_R1_001_2367706253


description: Library name: ENU57388B6G36_G6_neuro2185_S15_R1_001_2367705976


description: Library name: ENU57388B6G35_G5_neuro2185_S15_R1_001_2367706254


description: Library name: ENU57388B6G32_G2_neuro2185_S15_R1_001_2367706562


description: Library name: ENU57388B6G313_G12_neuro2185_S15_R1_001_2367705608


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