description: Library name: ENU58158B6G318_F11_neuro2205_S78_R1_001_2367708167


status: Public on Apr 11 2026

type: SRA

city: South San Francisco

first: Mitchell

last: Rezzonico

state: CA

address: 1 DNA Way

country: USA

institute: Genentech

description: Library name: ENU58155B6G38_C5_neuro2204_S67_R1_001_2367708944

library_source: transcriptomic

data_processing:
RNA-seq reads were aligned to the mouse reference genome GRCm38 using GSNAP 74. Only uniquely mapping reads were carried forward to downstream analysis. Sample quality was further assessed on a per-plate basis based on total read counts, percentage of reads mapping to the reference genome, and percentage of reads mapping to exons. Reads predominantly mapped to transcript 3’ ends, as expected from the library preparation method.
Assembly: GRCm38

molecule: total RNA

organism: Mus musculus

source_name: Whole brain

growth_protocol: Founder C57BL/6NCrl males were treated with 3 x 100 mg/kg ENU (Sigma) and mutations bred to homozygosity in three generations. Generation 3 mice were collected usually between 8-12 weeks old, and an ear notch for DNA and brain samples were collected. Typically, 12 siblings from each pedigree were screened.

extract_protocol:
To homogenize the tissue, RNAlater was removed, and a 5 mm stainless steel bead (Qiagen) and 1.6 mL of Qiazol (Qiagen) was added. The tissue was homogenized using a Tissue Lyser II (Qiagen) for at least 30 minutes until a homogenous solution was confirmed. 400 µL of the homogenate solution was transferred into a new tube and allowed to sit for 5 minutes before adding 100 µL of chloroform (Sigma). The homogenate-chloroform mixture was shaken vigorously, allowed to stand for 2 minutes before centrifugation for 5 minutes at 4°C and 1000 x g. The top clear layer was removed and stored at -80 °C. For RNA extraction, 120 µL was transferred into a QIAgen S-block and processed on the QIAcube using the RNeasy 96 QIAcube HT Kit.
cDNA fragment libraries were constructed using the SMART-Seq® v4 3’ DE Kit (Takara Bio) following manufacturer’s recommendations, with minor modifications. Total RNA samples were processed in batches of 1152, distributed across twelve 96-well plates. For each sample, 4 ng total RNA was used as input for cDNA synthesis, then amplified for 8 cycles, with each sample in a row receiving a unique in-line index. Amplified cDNAs within a row were then combined into a 12-plex pool, cleaned up with Ampure XP beads (Beckman Coulter) and quantified using the Qubit HS DNA assay kit (Thermo Fisher Scientific). For each of the 96 cDNA pools generated from 12 plates, 400 pg were carried into Nextera XT library preparations (Illumina Inc.), each assigned with one of the 96 unique combinations of Forward and Reverse HT Index Primers included in the SMART-Seq® v4 3’ DE kit. After Ampure XP clean up and Qubit quantification, the 96 pools were combined in an equimolar manner to generate a 1152-plex Illumina library.  This library was concentrated with Ampure XP beads, quantified with Qubit and assessed on the TapeStation with the High Sensitivity D1000 assay (Agilent Technologies).

treatment_protocol: Mice were euthanized by cervical dislocation, then whole brains were collected. Each cerebellum was removed and the remaining brain matter was transferred to RNAlater and stored at 4C for up to 5 days.

tag: tissue

value: Whole brain

tag: partofsubset

value: FALSE


description: Library name: ENU58158B6G311_F8_neuro2205_S78_R1_001_2367708348


description: Library name: ENU58158B6G310_F7_neuro2205_S78_R1_001_2367708933


description: Library name: ENU58155B6G37_C4_neuro2204_S67_R1_001_2367708333


description: Library name: ENU58158B6G31_F1_neuro2205_S78_R1_001_2367708878


description: Library name: ENU58155B6G36_C1_neuro2204_S67_R1_001_2367708455


description: Library name: ENU58155B6G39_C6_neuro2204_S67_R1_001_2367708229


description: Library name: ENU58155B6G318_C12_neuro2204_S67_R1_001_2367707965


description: Library name: ENU58155B6G315_C9_neuro2204_S67_R1_001_2367707892


description: Library name: ENU58155B6G313_C3_neuro2204_S67_R1_001_2367708138


description: Library name: ENU58155B6G312_C2_neuro2204_S67_R1_001_2367708126


description: Library name: ENU58155B6G311_C8_neuro2204_S67_R1_001_2367708113


description: Library name: ENU58155B6G310_C7_neuro2204_S67_R1_001_2367708236


description: Library name: ENU58153B6G38_E8_neuro2207_S93_R1_001_2367708976


description: Library name: ENU58153B6G39_E9_neuro2207_S93_R1_001_2367707940


description: Library name: ENU58153B6G37_E5_neuro2207_S93_R1_001_2367708145


description: Library name: ENU58155B6G316_C10_neuro2204_S67_R1_001_2367708678


description: Library name: ENU58153B6G36_E4_neuro2207_S93_R1_001_2367708807


description: Library name: ENU58153B6G35_E3_neuro2207_S93_R1_001_2367708221


description: Library name: ENU58153B6G33_E6_neuro2207_S93_R1_001_2367708164


description: Library name: ENU58153B6G316_E11_neuro2207_S93_R1_001_2367708066


description: Library name: ENU58155B6G317_C11_neuro2204_S67_R1_001_2367708581


description: Library name: ENU58151B6G36_C7_neuro2201_S43_R1_001_2367708375


description: Library name: ENU58153B6G319_E12_neuro2207_S93_R1_001_2367708030


description: Library name: ENU58153B6G31_E1_neuro2207_S93_R1_001_2367708917


description: Library name: ENU58151B6G37_C8_neuro2201_S43_R1_001_2367708896


description: Library name: ENU58153B6G32_E2_neuro2207_S93_R1_001_2367708342


description: Library name: ENU58153B6G34_E7_neuro2207_S93_R1_001_2367708465


description: Library name: ENU58151B6G32_C2_neuro2201_S43_R1_001_2367707857


description: Library name: ENU58153B6G310_E10_neuro2207_S93_R1_001_2367708501


description: Library name: ENU58151B6G33_C4_neuro2201_S43_R1_001_2367708155


description: Library name: ENU58151B6G34_C5_neuro2201_S43_R1_001_2367708230


description: Library name: ENU58151B6G39_C9_neuro2201_S43_R1_001_2367708539


description: Library name: ENU58151B6G310_C10_neuro2201_S43_R1_001_2367708937


description: Library name: ENU58151B6G312_C12_neuro2201_S43_R1_001_2367708226


description: Library name: ENU58149B6G36_H7_neuro2206_S88_R1_001_2367707957


description: Library name: ENU58149B6G39_H3_neuro2206_S88_R1_001_2367708141


description: Library name: ENU58151B6G38_C3_neuro2201_S43_R1_001_2367708049


description: Library name: ENU58151B6G31_C1_neuro2201_S43_R1_001_2367708644


description: Library name: ENU58149B6G34_H5_neuro2206_S88_R1_001_2367708589


description: Library name: ENU58149B6G33_H4_neuro2206_S88_R1_001_2367708444


description: Library name: ENU58149B6G31_H1_neuro2206_S88_R1_001_2367708534


description: Library name: ENU58149B6G32_H2_neuro2206_S88_R1_001_2367708981


description: Library name: ENU58151B6G311_C11_neuro2201_S43_R1_001_2367707894


description: Library name: ENU58149B6G35_H6_neuro2206_S88_R1_001_2367708955


description: Library name: ENU58149B6G315_H12_neuro2206_S88_R1_001_2367708117


description: Library name: ENU58149B6G314_H11_neuro2206_S88_R1_001_2367708959


description: Library name: ENU58149B6G312_H9_neuro2206_S88_R1_001_2367708560


description: Library name: ENU58147B6G39_A7_neuro2204_S65_R1_001_2367708748


description: Library name: ENU58147B6G38_A6_neuro2204_S65_R1_001_2367708002


description: Library name: ENU58147B6G37_A5_neuro2204_S65_R1_001_2367708107


description: Library name: ENU58149B6G311_H8_neuro2206_S88_R1_001_2367708813


description: Library name: ENU58149B6G313_H10_neuro2206_S88_R1_001_2367708218


description: Library name: ENU58147B6G36_A4_neuro2204_S65_R1_001_2367708241


description: Library name: ENU58147B6G35_A3_neuro2204_S65_R1_001_2367708340


description: Library name: ENU58147B6G32_A2_neuro2204_S65_R1_001_2367708913


description: Library name: ENU58147B6G313_A11_neuro2204_S65_R1_001_2367708853


description: Library name: ENU58147B6G314_A12_neuro2204_S65_R1_001_2367708311


description: Library name: ENU58147B6G311_A9_neuro2204_S65_R1_001_2367708153


description: Library name: ENU58147B6G31_A1_neuro2204_S65_R1_001_2367708399


description: Library name: ENU58147B6G310_A8_neuro2204_S65_R1_001_2367708593


description: Library name: ENU58146B6G39_E9_neuro2206_S85_R1_001_2367708743


description: Library name: ENU58147B6G312_A10_neuro2204_S65_R1_001_2367707918


description: Library name: ENU58146B6G37_E3_neuro2206_S85_R1_001_2367708319


description: Library name: ENU58146B6G36_E2_neuro2206_S85_R1_001_2367708203


description: Library name: ENU58146B6G35_E8_neuro2206_S85_R1_001_2367708566


description: Library name: ENU58146B6G33_E6_neuro2206_S85_R1_001_2367708006


description: Library name: ENU58146B6G34_E7_neuro2206_S85_R1_001_2367708272


description: Library name: ENU58146B6G32_E5_neuro2206_S85_R1_001_2367708152


description: Library name: ENU58146B6G38_E4_neuro2206_S85_R1_001_2367707866


description: Library name: ENU58146B6G312_E12_neuro2206_S85_R1_001_2367708927


description: Library name: ENU58146B6G31_E1_neuro2206_S85_R1_001_2367708257


description: Library name: ENU58146B6G310_E10_neuro2206_S85_R1_001_2367708016


description: Library name: ENU58145B6G36_G6_neuro2199_S31_R1_001_2367708524


description: Library name: ENU58145B6G35_G5_neuro2199_S31_R1_001_2367708388


description: Library name: ENU58146B6G311_E11_neuro2206_S85_R1_001_2367708172


description: Library name: ENU58145B6G32_G2_neuro2199_S31_R1_001_2367708441


description: Library name: ENU58145B6G33_G3_neuro2199_S31_R1_001_2367708353


description: Library name: ENU58145B6G316_G10_neuro2199_S31_R1_001_2367707998


description: Library name: ENU58145B6G315_G9_neuro2199_S31_R1_001_2367708641


description: Library name: ENU58145B6G319_G12_neuro2199_S31_R1_001_2367708096


description: Library name: ENU58145B6G31_G1_neuro2199_S31_R1_001_2367708898


description: Library name: ENU58145B6G314_G8_neuro2199_S31_R1_001_2367708294


description: Library name: ENU58144B6G39_G11_neuro2201_S47_R1_001_2367708291


description: Library name: ENU58145B6G313_G7_neuro2199_S31_R1_001_2367708891


description: Library name: ENU58145B6G318_G11_neuro2199_S31_R1_001_2367708965


description: Library name: ENU58144B6G38_G10_neuro2201_S47_R1_001_2367708101


description: Library name: ENU58144B6G37_G9_neuro2201_S47_R1_001_2367708086


description: Library name: ENU58144B6G36_G5_neuro2201_S47_R1_001_2367708362


description: Library name: ENU58144B6G35_G4_neuro2201_S47_R1_001_2367708473


description: Library name: ENU58144B6G31_G1_neuro2201_S47_R1_001_2367708419


description: Library name: ENU58144B6G313_G8_neuro2201_S47_R1_001_2367708802


description: Library name: ENU58144B6G311_G6_neuro2201_S47_R1_001_2367708351


description: Library name: ENU58144B6G312_G7_neuro2201_S47_R1_001_2367707885


description: Library name: ENU58144B6G33_G3_neuro2201_S47_R1_001_2367708750


description: Library name: ENU58143B6G39_B9_neuro2201_S42_R1_001_2367708861


description: Library name: ENU58144B6G310_G12_neuro2201_S47_R1_001_2367708323


description: Library name: ENU58144B6G32_G2_neuro2201_S47_R1_001_2367708497


description: Library name: ENU58143B6G36_B6_neuro2201_S42_R1_001_2367708252


description: Library name: ENU58143B6G38_B8_neuro2201_S42_R1_001_2367708059


description: Library name: ENU58143B6G34_B4_neuro2201_S42_R1_001_2367707920


description: Library name: ENU58143B6G311_B11_neuro2201_S42_R1_001_2367708130


description: Library name: ENU58143B6G37_B7_neuro2201_S42_R1_001_2367708036


description: Library name: ENU58143B6G312_B12_neuro2201_S42_R1_001_2367708097


description: Library name: ENU58143B6G31_B1_neuro2201_S42_R1_001_2367708073


description: Library name: ENU58143B6G35_B5_neuro2201_S42_R1_001_2367708382


description: Library name: ENU58143B6G32_B2_neuro2201_S42_R1_001_2367708374


description: Library name: ENU58143B6G33_B3_neuro2201_S42_R1_001_2367708571


description: Library name: ENU58139B6G34_H1_neuro2200_S40_R1_001_2367708647


description: Library name: ENU58139B6G38_H2_neuro2200_S40_R1_001_2367708582


description: Library name: ENU58139B6G39_H3_neuro2200_S40_R1_001_2367708738


description: Library name: ENU58139B6G33_H9_neuro2200_S40_R1_001_2367708313


description: Library name: ENU58139B6G31_H7_neuro2200_S40_R1_001_2367708554


description: Library name: ENU58139B6G318_H11_neuro2200_S40_R1_001_2367708961


description: Library name: ENU58139B6G32_H8_neuro2200_S40_R1_001_2367707865


description: Library name: ENU58139B6G317_H10_neuro2200_S40_R1_001_2367707990


description: Library name: ENU58143B6G310_B10_neuro2201_S42_R1_001_2367708398


description: Library name: ENU58139B6G319_H12_neuro2200_S40_R1_001_2367708121


description: Library name: ENU58139B6G312_H6_neuro2200_S40_R1_001_2367708223


description: Library name: ENU58138B6G38_F8_neuro2199_S30_R1_001_2367708935


description: Library name: ENU58139B6G311_H5_neuro2200_S40_R1_001_2367708371


description: Library name: ENU58138B6G35_F5_neuro2199_S30_R1_001_2367708302


description: Library name: ENU58138B6G36_F6_neuro2199_S30_R1_001_2367708269


description: Library name: ENU58138B6G34_F4_neuro2199_S30_R1_001_2367708832


description: Library name: ENU58138B6G312_F10_neuro2199_S30_R1_001_2367708912


description: Library name: ENU58138B6G313_F11_neuro2199_S30_R1_001_2367707891


description: Library name: ENU58137B6G36_B5_neuro2205_S74_R1_001_2367707975


description: Library name: ENU58138B6G314_F12_neuro2199_S30_R1_001_2367708381


description: Library name: ENU58138B6G33_F3_neuro2199_S30_R1_001_2367708093


description: Library name: ENU58138B6G32_F2_neuro2199_S30_R1_001_2367708087


description: Library name: ENU58139B6G310_H4_neuro2200_S40_R1_001_2367708283


description: Library name: ENU58138B6G311_F9_neuro2199_S30_R1_001_2367708208


description: Library name: ENU58137B6G35_B4_neuro2205_S74_R1_001_2367708964


description: Library name: ENU58137B6G33_B2_neuro2205_S74_R1_001_2367708540


description: Library name: ENU58137B6G34_B3_neuro2205_S74_R1_001_2367708808


description: Library name: ENU58138B6G37_F7_neuro2199_S30_R1_001_2367708516


description: Library name: ENU58137B6G31_B1_neuro2205_S74_R1_001_2367708990


description: Library name: ENU58137B6G323_B12_neuro2205_S74_R1_001_2367708886


description: Library name: ENU58137B6G314_B10_neuro2205_S74_R1_001_2367708989


description: Library name: ENU58137B6G315_B11_neuro2205_S74_R1_001_2367708355


description: Library name: ENU58138B6G31_F1_neuro2199_S30_R1_001_2367709000


description: Library name: ENU58137B6G312_B8_neuro2205_S74_R1_001_2367708851


description: Library name: ENU58137B6G313_B9_neuro2205_S74_R1_001_2367708969


description: Library name: ENU58137B6G311_B7_neuro2205_S74_R1_001_2367708088


description: Library name: ENU58134B6G39_B7_neuro2200_S34_R1_001_2367707947


description: Library name: ENU58137B6G310_B6_neuro2205_S74_R1_001_2367708469


description: Library name: ENU58134B6G35_B4_neuro2200_S34_R1_001_2367708745


description: Library name: ENU58134B6G37_B6_neuro2200_S34_R1_001_2367708131


description: Library name: ENU58151B6G35_C6_neuro2201_S43_R1_001_2367708233


description: Library name: ENU58134B6G33_B3_neuro2200_S34_R1_001_2367708479


description: Library name: ENU58134B6G36_B5_neuro2200_S34_R1_001_2367708885


description: Library name: ENU58134B6G31_B1_neuro2200_S34_R1_001_2367708506


description: Library name: ENU58128B6G37_F6_neuro2201_S46_R1_001_2367708648


description: Library name: ENU58134B6G311_B9_neuro2200_S34_R1_001_2367708968


description: Library name: ENU58134B6G312_B10_neuro2200_S34_R1_001_2367708925


description: Library name: ENU58134B6G32_B2_neuro2200_S34_R1_001_2367708819


description: Library name: ENU58145B6G34_G4_neuro2199_S31_R1_001_2367708903


description: Library name: ENU58134B6G313_B11_neuro2200_S34_R1_001_2367708626


description: Library name: ENU58134B6G310_B8_neuro2200_S34_R1_001_2367707922


description: Library name: ENU58128B6G35_F4_neuro2201_S46_R1_001_2367708899


description: Library name: ENU58128B6G32_F2_neuro2201_S46_R1_001_2367708511


description: Library name: ENU58128B6G314_F11_neuro2201_S46_R1_001_2367708091


description: Library name: ENU58128B6G311_F8_neuro2201_S46_R1_001_2367708339


description: Library name: ENU58128B6G312_F9_neuro2201_S46_R1_001_2367708502


description: Library name: ENU58128B6G313_F10_neuro2201_S46_R1_001_2367708866


description: Library name: ENU58128B6G36_F5_neuro2201_S46_R1_001_2367708552


description: Library name: ENU58128B6G310_F7_neuro2201_S46_R1_001_2367707960


description: Library name: ENU58128B6G315_F12_neuro2201_S46_R1_001_2367708403


description: Library name: ENU58128B6G31_F1_neuro2201_S46_R1_001_2367708185


description: Library name: ENU58119B6G38_B8_neuro2206_S82_R1_001_2367708755


description: Library name: ENU58119B6G39_B9_neuro2206_S82_R1_001_2367707871


description: Library name: ENU58119B6G37_B7_neuro2206_S82_R1_001_2367707964


description: Library name: ENU58119B6G35_B5_neuro2206_S82_R1_001_2367708423


description: Library name: ENU58119B6G36_B6_neuro2206_S82_R1_001_2367708864


description: Library name: ENU58119B6G33_B2_neuro2206_S82_R1_001_2367708301


description: Library name: ENU58119B6G34_B3_neuro2206_S82_R1_001_2367708727


description: Library name: ENU58119B6G32_B4_neuro2206_S82_R1_001_2367708321


description: Library name: ENU58119B6G314_B12_neuro2206_S82_R1_001_2367708024


description: Library name: ENU58115B6G33_C3_neuro2199_S27_R1_001_2367708824


description: Library name: ENU58115B6G38_C5_neuro2199_S27_R1_001_2367708594


description: Library name: ENU58115B6G34_C4_neuro2199_S27_R1_001_2367708209


description: Library name: ENU58115B6G39_C6_neuro2199_S27_R1_001_2367708188


description: Library name: ENU58119B6G31_B1_neuro2206_S82_R1_001_2367707981


description: Library name: ENU58115B6G32_C2_neuro2199_S27_R1_001_2367708610


description: Library name: ENU58115B6G318_C12_neuro2199_S27_R1_001_2367708055


description: Library name: ENU58119B6G310_B10_neuro2206_S82_R1_001_2367708879


description: Library name: ENU58115B6G316_C10_neuro2199_S27_R1_001_2367708605


description: Library name: ENU58119B6G313_B11_neuro2206_S82_R1_001_2367708920


description: Library name: ENU58115B6G31_C1_neuro2199_S27_R1_001_2367708169


description: Library name: ENU58115B6G317_C11_neuro2199_S27_R1_001_2367708706


description: Library name: ENU58115B6G314_C8_neuro2199_S27_R1_001_2367708764


description: Library name: ENU58114B6G37_E7_neuro2199_S29_R1_001_2367707925


description: Library name: ENU58115B6G313_C7_neuro2199_S27_R1_001_2367708525


description: Library name: ENU58114B6G36_E6_neuro2199_S29_R1_001_2367708494


description: Library name: ENU58114B6G35_E5_neuro2199_S29_R1_001_2367708118


description: Library name: ENU58114B6G33_E3_neuro2199_S29_R1_001_2367708551


description: Library name: ENU58114B6G32_E2_neuro2199_S29_R1_001_2367708043


description: Library name: ENU58114B6G39_E9_neuro2199_S29_R1_001_2367707962


description: Library name: ENU58114B6G38_E8_neuro2199_S29_R1_001_2367708868


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