description: Library name: ENU58552B6G34_C5_neuro2231_S27_R1_001_2367730752


status: Public on Apr 11 2026

type: SRA

city: South San Francisco

first: Mitchell

last: Rezzonico

state: CA

address: 1 DNA Way

country: USA

institute: Genentech

description: Library name: ENU58552B6G35_C6_neuro2231_S27_R1_001_2367731250

library_source: transcriptomic

data_processing:
RNA-seq reads were aligned to the mouse reference genome GRCm38 using GSNAP 74. Only uniquely mapping reads were carried forward to downstream analysis. Sample quality was further assessed on a per-plate basis based on total read counts, percentage of reads mapping to the reference genome, and percentage of reads mapping to exons. Reads predominantly mapped to transcript 3’ ends, as expected from the library preparation method.
Assembly: GRCm38

molecule: total RNA

organism: Mus musculus

source_name: Whole brain

growth_protocol: Founder C57BL/6NCrl males were treated with 3 x 100 mg/kg ENU (Sigma) and mutations bred to homozygosity in three generations. Generation 3 mice were collected usually between 8-12 weeks old, and an ear notch for DNA and brain samples were collected. Typically, 12 siblings from each pedigree were screened.

extract_protocol:
To homogenize the tissue, RNAlater was removed, and a 5 mm stainless steel bead (Qiagen) and 1.6 mL of Qiazol (Qiagen) was added. The tissue was homogenized using a Tissue Lyser II (Qiagen) for at least 30 minutes until a homogenous solution was confirmed. 400 µL of the homogenate solution was transferred into a new tube and allowed to sit for 5 minutes before adding 100 µL of chloroform (Sigma). The homogenate-chloroform mixture was shaken vigorously, allowed to stand for 2 minutes before centrifugation for 5 minutes at 4°C and 1000 x g. The top clear layer was removed and stored at -80 °C. For RNA extraction, 120 µL was transferred into a QIAgen S-block and processed on the QIAcube using the RNeasy 96 QIAcube HT Kit.
cDNA fragment libraries were constructed using the SMART-Seq® v4 3’ DE Kit (Takara Bio) following manufacturer’s recommendations, with minor modifications. Total RNA samples were processed in batches of 1152, distributed across twelve 96-well plates. For each sample, 4 ng total RNA was used as input for cDNA synthesis, then amplified for 8 cycles, with each sample in a row receiving a unique in-line index. Amplified cDNAs within a row were then combined into a 12-plex pool, cleaned up with Ampure XP beads (Beckman Coulter) and quantified using the Qubit HS DNA assay kit (Thermo Fisher Scientific). For each of the 96 cDNA pools generated from 12 plates, 400 pg were carried into Nextera XT library preparations (Illumina Inc.), each assigned with one of the 96 unique combinations of Forward and Reverse HT Index Primers included in the SMART-Seq® v4 3’ DE kit. After Ampure XP clean up and Qubit quantification, the 96 pools were combined in an equimolar manner to generate a 1152-plex Illumina library.  This library was concentrated with Ampure XP beads, quantified with Qubit and assessed on the TapeStation with the High Sensitivity D1000 assay (Agilent Technologies).

treatment_protocol: Mice were euthanized by cervical dislocation, then whole brains were collected. Each cerebellum was removed and the remaining brain matter was transferred to RNAlater and stored at 4C for up to 5 days.

tag: tissue

value: Whole brain

tag: partofsubset

value: FALSE


description: Library name: ENU59085B6G317_H10_neuro2239_S96_R1_001_2367731526


description: Library name: ENU58552B6G32_C3_neuro2231_S27_R1_001_2367731166


description: Library name: ENU58552B6G31_C1_neuro2231_S27_R1_001_2367731556


description: Library name: ENU58552B6G33_C4_neuro2231_S27_R1_001_2367731680


description: Library name: ENU58552B6G318_C11_neuro2231_S27_R1_001_2367731686


description: Library name: ENU58552B6G319_C12_neuro2231_S27_R1_001_2367731485


description: Library name: ENU58552B6G317_C10_neuro2231_S27_R1_001_2367731210


description: Library name: ENU58552B6G316_C9_neuro2231_S27_R1_001_2367731325


description: Library name: ENU58552B6G314_C2_neuro2231_S27_R1_001_2367731572


description: Library name: ENU58541B6G322_A12_neuro2232_S33_R1_001_2367731302


description: Library name: ENU58541B6G321_A11_neuro2232_S33_R1_001_2367730991


description: Library name: ENU58541B6G316_A6_neuro2232_S33_R1_001_2367731312


description: Library name: ENU58541B6G318_A8_neuro2232_S33_R1_001_2367730735


description: Library name: ENU58541B6G319_A9_neuro2232_S33_R1_001_2367731597


description: Library name: ENU58541B6G312_A4_neuro2232_S33_R1_001_2367731629


description: Library name: ENU58541B6G313_A5_neuro2232_S33_R1_001_2367730767


description: Library name: ENU58541B6G39_A1_neuro2232_S33_R1_001_2367730893


description: Library name: ENU58541B6G311_A3_neuro2232_S33_R1_001_2367730954


description: Library name: ENU58541B6G317_A7_neuro2232_S33_R1_001_2367731397


description: Library name: ENU58541B6G310_A2_neuro2232_S33_R1_001_2367731812


description: Library name: ENU58552B6G315_C8_neuro2231_S27_R1_001_2367731548


description: Library name: ENU58535B6G37_H6_neuro2231_S32_R1_001_2367730868


description: Library name: ENU58535B6G321_H8_neuro2231_S32_R1_001_2367731418


description: Library name: ENU58535B6G36_H5_neuro2231_S32_R1_001_2367731635


description: Library name: ENU58535B6G35_H4_neuro2231_S32_R1_001_2367730853


description: Library name: ENU58535B6G38_H7_neuro2231_S32_R1_001_2367730924


description: Library name: ENU58535B6G318_H11_neuro2231_S32_R1_001_2367731739


description: Library name: ENU58535B6G322_H12_neuro2231_S32_R1_001_2367731240


description: Library name: ENU58535B6G33_H1_neuro2231_S32_R1_001_2367730740


description: Library name: ENU58535B6G34_H2_neuro2231_S32_R1_001_2367730865


description: Library name: ENU58535B6G317_H10_neuro2231_S32_R1_001_2367731414


description: Library name: ENU58529B6G38_F2_neuro2233_S46_R1_001_2367731832


description: Library name: ENU58529B6G37_F1_neuro2233_S46_R1_001_2367731607


description: Library name: ENU58529B6G321_F12_neuro2233_S46_R1_001_2367731700


description: Library name: ENU58535B6G315_H3_neuro2231_S32_R1_001_2367731075


description: Library name: ENU58529B6G319_F10_neuro2233_S46_R1_001_2367731435


description: Library name: ENU58529B6G316_F7_neuro2233_S46_R1_001_2367731366


description: Library name: ENU58535B6G316_H9_neuro2231_S32_R1_001_2367731040


description: Library name: ENU58529B6G39_F3_neuro2233_S46_R1_001_2367731311


description: Library name: ENU58529B6G320_F11_neuro2233_S46_R1_001_2367731186


description: Library name: ENU58529B6G311_F5_neuro2233_S46_R1_001_2367731614


description: Library name: ENU58529B6G317_F8_neuro2233_S46_R1_001_2367731058


description: Library name: ENU58524B6G38_A2_neuro2233_S41_R1_001_2367731770


description: Library name: ENU58529B6G318_F9_neuro2233_S46_R1_001_2367731365


description: Library name: ENU58529B6G310_F4_neuro2233_S46_R1_001_2367731343


description: Library name: ENU58524B6G39_A3_neuro2233_S41_R1_001_2367731310


description: Library name: ENU58524B6G34_A6_neuro2233_S41_R1_001_2367731747


description: Library name: ENU58524B6G33_A5_neuro2233_S41_R1_001_2367731702


description: Library name: ENU58524B6G31_A1_neuro2233_S41_R1_001_2367730934


description: Library name: ENU58524B6G314_A10_neuro2233_S41_R1_001_2367731471


description: Library name: ENU58524B6G32_A4_neuro2233_S41_R1_001_2367731645


description: Library name: ENU58524B6G315_A11_neuro2233_S41_R1_001_2367730947


description: Library name: ENU58524B6G316_A12_neuro2233_S41_R1_001_2367731774


description: Library name: ENU58524B6G313_A9_neuro2233_S41_R1_001_2367731724


description: Library name: ENU58523B6G38_H9_neuro2232_S40_R1_001_2367731668


description: Library name: ENU58523B6G39_H10_neuro2232_S40_R1_001_2367731486


description: Library name: ENU58524B6G311_A7_neuro2233_S41_R1_001_2367731236


description: Library name: ENU58523B6G315_H4_neuro2232_S40_R1_001_2367731574


description: Library name: ENU58523B6G316_H5_neuro2232_S40_R1_001_2367730809


description: Library name: ENU58524B6G312_A8_neuro2233_S41_R1_001_2367731413


description: Library name: ENU58523B6G36_H7_neuro2232_S40_R1_001_2367730897


description: Library name: ENU58523B6G313_H2_neuro2232_S40_R1_001_2367730925


description: Library name: ENU58523B6G311_H12_neuro2232_S40_R1_001_2367730940


description: Library name: ENU58523B6G314_H3_neuro2232_S40_R1_001_2367730813


description: Library name: ENU58516B6G39_G8_neuro2231_S31_R1_001_2367731027


description: Library name: ENU58523B6G37_H8_neuro2232_S40_R1_001_2367731710


description: Library name: ENU58523B6G310_H11_neuro2232_S40_R1_001_2367731380


description: Library name: ENU58516B6G38_G7_neuro2231_S31_R1_001_2367730945


description: Library name: ENU58516B6G37_G6_neuro2231_S31_R1_001_2367730756


description: Library name: ENU58516B6G33_G5_neuro2231_S31_R1_001_2367731594


description: Library name: ENU58523B6G312_H1_neuro2232_S40_R1_001_2367731874


description: Library name: ENU58516B6G35_G3_neuro2231_S31_R1_001_2367731689


description: Library name: ENU58516B6G34_G2_neuro2231_S31_R1_001_2367731764


description: Library name: ENU58516B6G32_G4_neuro2231_S31_R1_001_2367731337


description: Library name: ENU58513B6G36_B4_neuro2233_S42_R1_001_2367731097


description: Library name: ENU58516B6G310_G9_neuro2231_S31_R1_001_2367731759


description: Library name: ENU58513B6G33_B3_neuro2233_S42_R1_001_2367731256


description: Library name: ENU58516B6G31_G1_neuro2231_S31_R1_001_2367731321


description: Library name: ENU58513B6G35_B6_neuro2233_S42_R1_001_2367731697


description: Library name: ENU58513B6G34_B5_neuro2233_S42_R1_001_2367731386


description: Library name: ENU58513B6G31_B1_neuro2233_S42_R1_001_2367731376


description: Library name: ENU58516B6G319_G11_neuro2231_S31_R1_001_2367731733


description: Library name: ENU58516B6G320_G12_neuro2231_S31_R1_001_2367731837


description: Library name: ENU58513B6G32_B2_neuro2233_S42_R1_001_2367731718


description: Library name: ENU58508B6G35_G5_neuro2238_S87_R1_001_2367730879


description: Library name: ENU58516B6G318_G10_neuro2231_S31_R1_001_2367731023


description: Library name: ENU58529B6G312_F6_neuro2233_S46_R1_001_2367731841


description: Library name: ENU58508B6G39_G9_neuro2238_S87_R1_001_2367730987


description: Library name: ENU58508B6G36_G6_neuro2238_S87_R1_001_2367731348


description: Library name: ENU58508B6G31_G1_neuro2238_S87_R1_001_2367731279


description: Library name: ENU58508B6G38_G8_neuro2238_S87_R1_001_2367730953


description: Library name: ENU58508B6G37_G7_neuro2238_S87_R1_001_2367730773


description: Library name: ENU58508B6G34_G4_neuro2238_S87_R1_001_2367731524


description: Library name: ENU58508B6G312_G11_neuro2238_S87_R1_001_2367730873


description: Library name: ENU58508B6G32_G2_neuro2238_S87_R1_001_2367731848


description: Library name: ENU58508B6G33_G3_neuro2238_S87_R1_001_2367731113


description: Library name: ENU58508B6G329_G12_neuro2238_S87_R1_001_2367731217


description: Library name: ENU58508B6G311_G10_neuro2238_S87_R1_001_2367731204


description: Library name: ENU58504B6G39_B8_neuro2237_S74_R1_001_2367731021


description: Library name: ENU58504B6G38_B7_neuro2237_S74_R1_001_2367731581


description: Library name: ENU58504B6G33_B2_neuro2237_S74_R1_001_2367730946


description: Library name: ENU58504B6G32_B1_neuro2237_S74_R1_001_2367731474


description: Library name: ENU58504B6G34_B3_neuro2237_S74_R1_001_2367731448


description: Library name: ENU58504B6G35_B4_neuro2237_S74_R1_001_2367731560


description: Library name: ENU58504B6G36_B5_neuro2237_S74_R1_001_2367731830


description: Library name: ENU58504B6G313_B12_neuro2237_S74_R1_001_2367730775


description: Library name: ENU58504B6G311_B10_neuro2237_S74_R1_001_2367731068


description: Library name: ENU58504B6G310_B9_neuro2237_S74_R1_001_2367731483


description: Library name: ENU58503B6G34_G6_neuro2236_S71_R1_001_2367730759


description: Library name: ENU58504B6G312_B11_neuro2237_S74_R1_001_2367731125


description: Library name: ENU58503B6G33_G5_neuro2236_S71_R1_001_2367730931


description: Library name: ENU58504B6G37_B6_neuro2237_S74_R1_001_2367731283


description: Library name: ENU58503B6G318_G12_neuro2236_S71_R1_001_2367731083


description: Library name: ENU58503B6G31_G1_neuro2236_S71_R1_001_2367731017


description: Library name: ENU58503B6G316_G10_neuro2236_S71_R1_001_2367731276


description: Library name: ENU58503B6G314_G8_neuro2236_S71_R1_001_2367730826


description: Library name: ENU58503B6G32_G4_neuro2236_S71_R1_001_2367731591


description: Library name: ENU58503B6G313_G3_neuro2236_S71_R1_001_2367731032


description: Library name: ENU58500B6G38_G8_neuro2232_S39_R1_001_2367731734


description: Library name: ENU58503B6G35_G7_neuro2236_S71_R1_001_2367731274


description: Library name: ENU58503B6G312_G2_neuro2236_S71_R1_001_2367731814


description: Library name: ENU58503B6G317_G11_neuro2236_S71_R1_001_2367730747


description: Library name: ENU58503B6G315_G9_neuro2236_S71_R1_001_2367730769


description: Library name: ENU58500B6G36_G6_neuro2232_S39_R1_001_2367731327


description: Library name: ENU58500B6G37_G7_neuro2232_S39_R1_001_2367731399


description: Library name: ENU58523B6G317_H6_neuro2232_S40_R1_001_2367730827


description: Library name: ENU58500B6G35_G5_neuro2232_S39_R1_001_2367731808


description: Library name: ENU58500B6G32_G2_neuro2232_S39_R1_001_2367730811


description: Library name: ENU58500B6G313_G4_neuro2232_S39_R1_001_2367731523


description: Library name: ENU58500B6G311_G11_neuro2232_S39_R1_001_2367731676


description: Library name: ENU58500B6G314_G12_neuro2232_S39_R1_001_2367731314


description: Library name: ENU58500B6G39_G9_neuro2232_S39_R1_001_2367731721


description: Library name: ENU58500B6G33_G3_neuro2232_S39_R1_001_2367730753


description: Library name: ENU58500B6G31_G1_neuro2232_S39_R1_001_2367730941


description: Library name: ENU58499B6G39_B7_neuro2232_S34_R1_001_2367731169


description: Library name: ENU58500B6G310_G10_neuro2232_S39_R1_001_2367731588


description: Library name: ENU58499B6G34_B2_neuro2232_S34_R1_001_2367731768


description: Library name: ENU58499B6G36_B4_neuro2232_S34_R1_001_2367731063


description: Library name: ENU58499B6G35_B3_neuro2232_S34_R1_001_2367731049


description: Library name: ENU58499B6G37_B5_neuro2232_S34_R1_001_2367731487


description: Library name: ENU58499B6G38_B6_neuro2232_S34_R1_001_2367730850


description: Library name: ENU58499B6G314_B10_neuro2232_S34_R1_001_2367731139


description: Library name: ENU58499B6G323_B12_neuro2232_S34_R1_001_2367730779


description: Library name: ENU58499B6G33_B1_neuro2232_S34_R1_001_2367731434


description: Library name: ENU58499B6G310_B8_neuro2232_S34_R1_001_2367730882


description: Library name: ENU58499B6G317_B11_neuro2232_S34_R1_001_2367731422


description: Library name: ENU58499B6G311_B9_neuro2232_S34_R1_001_2367731114


description: Library name: ENU58496B6G38_B7_neuro2231_S26_R1_001_2367730976


description: Library name: ENU58496B6G35_B5_neuro2231_S26_R1_001_2367730872


description: Library name: ENU58496B6G33_B3_neuro2231_S26_R1_001_2367731353


description: Library name: ENU58496B6G34_B4_neuro2231_S26_R1_001_2367731020


description: Library name: ENU58496B6G32_B2_neuro2231_S26_R1_001_2367731723


description: Library name: ENU58496B6G313_B11_neuro2231_S26_R1_001_2367730786


description: Library name: ENU58496B6G31_B1_neuro2231_S26_R1_001_2367731308


description: Library name: ENU58496B6G37_B6_neuro2231_S26_R1_001_2367731185


description: Library name: ENU58496B6G312_B10_neuro2231_S26_R1_001_2367731117


description: Library name: ENU58490B6G38_H9_neuro2237_S80_R1_001_2367730988


description: Library name: ENU58496B6G311_B9_neuro2231_S26_R1_001_2367731484


description: Library name: ENU58490B6G34_H4_neuro2237_S80_R1_001_2367731235


description: Library name: ENU58496B6G310_B8_neuro2231_S26_R1_001_2367731838


description: Library name: ENU58490B6G33_H3_neuro2237_S80_R1_001_2367731420


description: Library name: ENU58490B6G32_H2_neuro2237_S80_R1_001_2367730997


description: Library name: ENU58490B6G39_H10_neuro2237_S80_R1_001_2367731851


description: Library name: ENU58496B6G314_B12_neuro2231_S26_R1_001_2367731377


description: Library name: ENU58490B6G313_H6_neuro2237_S80_R1_001_2367731282


description: Library name: ENU58490B6G31_H1_neuro2237_S80_R1_001_2367731436


description: Library name: ENU58490B6G311_H12_neuro2237_S80_R1_001_2367730837


description: Library name: ENU58490B6G315_H8_neuro2237_S80_R1_001_2367731616


description: Library name: ENU58487B6G36_D5_neuro2237_S76_R1_001_2367731197


description: Library name: ENU58487B6G39_D7_neuro2237_S76_R1_001_2367730981


description: Library name: ENU58487B6G37_D6_neuro2237_S76_R1_001_2367731849


description: Library name: ENU58487B6G35_D4_neuro2237_S76_R1_001_2367731336


description: Library name: ENU58490B6G314_H7_neuro2237_S80_R1_001_2367731742


description: Library name: ENU58487B6G34_D3_neuro2237_S76_R1_001_2367731439


description: Library name: ENU58487B6G33_D2_neuro2237_S76_R1_001_2367731580


description: Library name: ENU58490B6G310_H11_neuro2237_S80_R1_001_2367731535


description: Library name: ENU58487B6G310_D8_neuro2237_S76_R1_001_2367730849


description: Library name: ENU58487B6G311_D9_neuro2237_S76_R1_001_2367731458


description: Library name: ENU58487B6G32_D1_neuro2237_S76_R1_001_2367731199


description: Library name: ENU58482B6G35_E5_neuro2229_S13_R1_001_2367730834


description: Library name: ENU58482B6G36_E6_neuro2229_S13_R1_001_2367731794


description: Library name: ENU58487B6G313_D11_neuro2237_S76_R1_001_2367730791


description: Library name: ENU58482B6G34_E4_neuro2229_S13_R1_001_2367730936


description: Library name: ENU58487B6G312_D10_neuro2237_S76_R1_001_2367731372


description: Library name: ENU58487B6G314_D12_neuro2237_S76_R1_001_2367731161


description: Library name: ENU58490B6G312_H5_neuro2237_S80_R1_001_2367731528


description: Library name: ENU58482B6G33_E3_neuro2229_S13_R1_001_2367730820


description: Library name: ENU58482B6G316_E12_neuro2229_S13_R1_001_2367731443


description: Library name: ENU58482B6G31_E1_neuro2229_S13_R1_001_2367731051


description: Library name: ENU58482B6G315_E11_neuro2229_S13_R1_001_2367730884


description: Library name: ENU58482B6G314_E10_neuro2229_S13_R1_001_2367731589


description: Library name: ENU58482B6G313_E9_neuro2229_S13_R1_001_2367731038


description: Library name: ENU58482B6G312_E8_neuro2229_S13_R1_001_2367730845


description: Library name: ENU58482B6G32_E2_neuro2229_S13_R1_001_2367731509


description: Library name: ENU58480B6G38_D5_neuro2238_S84_R1_001_2367730891


description: Library name: ENU58480B6G39_D6_neuro2238_S84_R1_001_2367731190


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