title: ENU55132B6G35_G4_neuro2099_S87_R1_001

description: Library name: ENU55132B6G35_G4_neuro2099_S87_R1_001


title: ENU55132B6G32_G1_neuro2099_S87_R1_001

status: Public on Apr 11 2026

type: SRA

city: South San Francisco

first: Mitchell

last: Rezzonico

state: CA

address: 1 DNA Way

country: USA

institute: Genentech

description: Library name: ENU55132B6G32_G1_neuro2099_S87_R1_001

library_source: transcriptomic

data_processing:
RNA-seq reads were aligned to the mouse reference genome GRCm38 using GSNAP 74. Only uniquely mapping reads were carried forward to downstream analysis. Sample quality was further assessed on a per-plate basis based on total read counts, percentage of reads mapping to the reference genome, and percentage of reads mapping to exons. Reads predominantly mapped to transcript 3’ ends, as expected from the library preparation method.
Assembly: GRCm38

molecule: total RNA

organism: Mus musculus

source_name: Whole brain

growth_protocol: Founder C57BL/6NCrl males were treated with 3 x 100 mg/kg ENU (Sigma) and mutations bred to homozygosity in three generations. Generation 3 mice were collected usually between 8-12 weeks old, and an ear notch for DNA and brain samples were collected. Typically, 12 siblings from each pedigree were screened.

extract_protocol:
To homogenize the tissue, RNAlater was removed, and a 5 mm stainless steel bead (Qiagen) and 1.6 mL of Qiazol (Qiagen) was added. The tissue was homogenized using a Tissue Lyser II (Qiagen) for at least 30 minutes until a homogenous solution was confirmed. 400 µL of the homogenate solution was transferred into a new tube and allowed to sit for 5 minutes before adding 100 µL of chloroform (Sigma). The homogenate-chloroform mixture was shaken vigorously, allowed to stand for 2 minutes before centrifugation for 5 minutes at 4°C and 1000 x g. The top clear layer was removed and stored at -80 °C. For RNA extraction, 120 µL was transferred into a QIAgen S-block and processed on the QIAcube using the RNeasy 96 QIAcube HT Kit.
cDNA fragment libraries were constructed using the SMART-Seq® v4 3’ DE Kit (Takara Bio) following manufacturer’s recommendations, with minor modifications. Total RNA samples were processed in batches of 1152, distributed across twelve 96-well plates. For each sample, 4 ng total RNA was used as input for cDNA synthesis, then amplified for 8 cycles, with each sample in a row receiving a unique in-line index. Amplified cDNAs within a row were then combined into a 12-plex pool, cleaned up with Ampure XP beads (Beckman Coulter) and quantified using the Qubit HS DNA assay kit (Thermo Fisher Scientific). For each of the 96 cDNA pools generated from 12 plates, 400 pg were carried into Nextera XT library preparations (Illumina Inc.), each assigned with one of the 96 unique combinations of Forward and Reverse HT Index Primers included in the SMART-Seq® v4 3’ DE kit. After Ampure XP clean up and Qubit quantification, the 96 pools were combined in an equimolar manner to generate a 1152-plex Illumina library.  This library was concentrated with Ampure XP beads, quantified with Qubit and assessed on the TapeStation with the High Sensitivity D1000 assay (Agilent Technologies).

treatment_protocol: Mice were euthanized by cervical dislocation, then whole brains were collected. Each cerebellum was removed and the remaining brain matter was transferred to RNAlater and stored at 4C for up to 5 days.

tag: tissue

value: Whole brain

tag: partofsubset

value: FALSE


title: ENU55232B6G39_F5_neuro2092_S30_R1_001

description: Library name: ENU55232B6G39_F5_neuro2092_S30_R1_001


title: ENU55173B6G35_A2_neuro2092_S25_R1_001

description: Library name: ENU55173B6G35_A2_neuro2092_S25_R1_001


title: ENU55132B6G320_G12_neuro2099_S87_R1_001

description: Library name: ENU55132B6G320_G12_neuro2099_S87_R1_001


title: ENU55132B6G319_G11_neuro2099_S87_R1_001

description: Library name: ENU55132B6G319_G11_neuro2099_S87_R1_001


title: ENU55132B6G312_G9_neuro2099_S87_R1_001

description: Library name: ENU55132B6G312_G9_neuro2099_S87_R1_001


title: ENU55173B6G317_A11_neuro2092_S25_R1_001

description: Library name: ENU55173B6G317_A11_neuro2092_S25_R1_001


title: ENU55132B6G311_G8_neuro2099_S87_R1_001

description: Library name: ENU55132B6G311_G8_neuro2099_S87_R1_001


title: ENU55132B6G310_G7_neuro2099_S87_R1_001

description: Library name: ENU55132B6G310_G7_neuro2099_S87_R1_001


title: ENU55131B6G39_G7_neuro2089_S15_R1_001

description: Library name: ENU55131B6G39_G7_neuro2089_S15_R1_001


title: ENU55131B6G36_G6_neuro2089_S15_R1_001

description: Library name: ENU55131B6G36_G6_neuro2089_S15_R1_001


title: ENU55131B6G35_G5_neuro2089_S15_R1_001

description: Library name: ENU55131B6G35_G5_neuro2089_S15_R1_001


title: ENU55232B6G310_F6_neuro2092_S30_R1_001

description: Library name: ENU55232B6G310_F6_neuro2092_S30_R1_001


title: ENU55131B6G34_G4_neuro2089_S15_R1_001

description: Library name: ENU55131B6G34_G4_neuro2089_S15_R1_001


title: ENU55131B6G32_G2_neuro2089_S15_R1_001

description: Library name: ENU55131B6G32_G2_neuro2089_S15_R1_001


title: ENU55131B6G31_G1_neuro2089_S15_R1_001

description: Library name: ENU55131B6G31_G1_neuro2089_S15_R1_001


title: ENU55131B6G314_G12_neuro2089_S15_R1_001

description: Library name: ENU55131B6G314_G12_neuro2089_S15_R1_001


title: ENU55131B6G313_G11_neuro2089_S15_R1_001

description: Library name: ENU55131B6G313_G11_neuro2089_S15_R1_001


title: ENU55131B6G312_G10_neuro2089_S15_R1_001

description: Library name: ENU55131B6G312_G10_neuro2089_S15_R1_001


title: ENU55131B6G311_G9_neuro2089_S15_R1_001

description: Library name: ENU55131B6G311_G9_neuro2089_S15_R1_001


title: ENU55131B6G310_G8_neuro2089_S15_R1_001

description: Library name: ENU55131B6G310_G8_neuro2089_S15_R1_001


title: ENU55130B6G38_F6_neuro2089_S14_R1_001

description: Library name: ENU55130B6G38_F6_neuro2089_S14_R1_001


title: ENU55130B6G37_F5_neuro2089_S14_R1_001

description: Library name: ENU55130B6G37_F5_neuro2089_S14_R1_001


title: ENU55130B6G36_F4_neuro2089_S14_R1_001

description: Library name: ENU55130B6G36_F4_neuro2089_S14_R1_001


title: ENU55130B6G35_F3_neuro2089_S14_R1_001

description: Library name: ENU55130B6G35_F3_neuro2089_S14_R1_001


title: ENU55130B6G34_F2_neuro2089_S14_R1_001

description: Library name: ENU55130B6G34_F2_neuro2089_S14_R1_001


title: ENU55130B6G322_F11_neuro2089_S14_R1_001

description: Library name: ENU55130B6G322_F11_neuro2089_S14_R1_001


title: ENU55130B6G323_F12_neuro2089_S14_R1_001

description: Library name: ENU55130B6G323_F12_neuro2089_S14_R1_001


title: ENU55130B6G321_F10_neuro2089_S14_R1_001

description: Library name: ENU55130B6G321_F10_neuro2089_S14_R1_001


title: ENU55130B6G320_F9_neuro2089_S14_R1_001

description: Library name: ENU55130B6G320_F9_neuro2089_S14_R1_001


title: ENU55130B6G319_F8_neuro2089_S14_R1_001

description: Library name: ENU55130B6G319_F8_neuro2089_S14_R1_001


title: ENU55123B6G39_E1_neuro2088_S5_R1_001

description: Library name: ENU55123B6G39_E1_neuro2088_S5_R1_001


title: ENU55123B6G327_E12_neuro2088_S5_R1_001

description: Library name: ENU55123B6G327_E12_neuro2088_S5_R1_001


title: ENU55284B6G323_E8_neuro2097_S69_R1_001

description: Library name: ENU55284B6G323_E8_neuro2097_S69_R1_001


title: ENU55123B6G326_E11_neuro2088_S5_R1_001

description: Library name: ENU55123B6G326_E11_neuro2088_S5_R1_001


title: ENU55123B6G320_E7_neuro2088_S5_R1_001

description: Library name: ENU55123B6G320_E7_neuro2088_S5_R1_001


title: ENU55123B6G319_E10_neuro2088_S5_R1_001

description: Library name: ENU55123B6G319_E10_neuro2088_S5_R1_001


title: ENU55123B6G318_E9_neuro2088_S5_R1_001

description: Library name: ENU55123B6G318_E9_neuro2088_S5_R1_001


title: ENU55123B6G317_E8_neuro2088_S5_R1_001

description: Library name: ENU55123B6G317_E8_neuro2088_S5_R1_001


title: ENU55123B6G313_E5_neuro2088_S5_R1_001

description: Library name: ENU55123B6G313_E5_neuro2088_S5_R1_001


title: ENU55123B6G312_E4_neuro2088_S5_R1_001

description: Library name: ENU55123B6G312_E4_neuro2088_S5_R1_001


title: ENU55123B6G310_E2_neuro2088_S5_R1_001

description: Library name: ENU55123B6G310_E2_neuro2088_S5_R1_001


title: ENU55123B6G314_E6_neuro2088_S5_R1_001

description: Library name: ENU55123B6G314_E6_neuro2088_S5_R1_001


title: ENU55123B6G311_E3_neuro2088_S5_R1_001

description: Library name: ENU55123B6G311_E3_neuro2088_S5_R1_001


title: ENU55119B6G39_B9_neuro2093_S34_R1_001

description: Library name: ENU55119B6G39_B9_neuro2093_S34_R1_001


title: ENU55119B6G38_B8_neuro2093_S34_R1_001

description: Library name: ENU55119B6G38_B8_neuro2093_S34_R1_001


title: ENU55119B6G37_B7_neuro2093_S34_R1_001

description: Library name: ENU55119B6G37_B7_neuro2093_S34_R1_001


title: ENU55119B6G36_B6_neuro2093_S34_R1_001

description: Library name: ENU55119B6G36_B6_neuro2093_S34_R1_001


title: ENU55119B6G35_B5_neuro2093_S34_R1_001

description: Library name: ENU55119B6G35_B5_neuro2093_S34_R1_001


title: ENU55119B6G33_B3_neuro2093_S34_R1_001

description: Library name: ENU55119B6G33_B3_neuro2093_S34_R1_001


title: ENU55119B6G312_B12_neuro2093_S34_R1_001

description: Library name: ENU55119B6G312_B12_neuro2093_S34_R1_001


title: ENU55119B6G32_B2_neuro2093_S34_R1_001

description: Library name: ENU55119B6G32_B2_neuro2093_S34_R1_001


title: ENU55116B6G39_A4_neuro2088_S1_R1_001

description: Library name: ENU55116B6G39_A4_neuro2088_S1_R1_001


title: ENU55119B6G310_B10_neuro2093_S34_R1_001

description: Library name: ENU55119B6G310_B10_neuro2093_S34_R1_001


title: ENU55119B6G31_B1_neuro2093_S34_R1_001

description: Library name: ENU55119B6G31_B1_neuro2093_S34_R1_001


title: ENU55119B6G311_B11_neuro2093_S34_R1_001

description: Library name: ENU55119B6G311_B11_neuro2093_S34_R1_001


title: ENU55116B6G38_A3_neuro2088_S1_R1_001

description: Library name: ENU55116B6G38_A3_neuro2088_S1_R1_001


title: ENU55116B6G36_A7_neuro2088_S1_R1_001

description: Library name: ENU55116B6G36_A7_neuro2088_S1_R1_001


title: ENU55116B6G35_A6_neuro2088_S1_R1_001

description: Library name: ENU55116B6G35_A6_neuro2088_S1_R1_001


title: ENU55116B6G32_A2_neuro2088_S1_R1_001

description: Library name: ENU55116B6G32_A2_neuro2088_S1_R1_001


title: ENU55116B6G31_A1_neuro2088_S1_R1_001

description: Library name: ENU55116B6G31_A1_neuro2088_S1_R1_001


title: ENU55116B6G314_A12_neuro2088_S1_R1_001

description: Library name: ENU55116B6G314_A12_neuro2088_S1_R1_001


title: ENU55116B6G33_A5_neuro2088_S1_R1_001

description: Library name: ENU55116B6G33_A5_neuro2088_S1_R1_001


title: ENU55116B6G313_A11_neuro2088_S1_R1_001

description: Library name: ENU55116B6G313_A11_neuro2088_S1_R1_001


title: ENU55116B6G311_A10_neuro2088_S1_R1_001

description: Library name: ENU55116B6G311_A10_neuro2088_S1_R1_001


title: ENU55116B6G310_A9_neuro2088_S1_R1_001

description: Library name: ENU55116B6G310_A9_neuro2088_S1_R1_001


title: ENU55100B6G38_D3_neuro2091_S20_R1_001

description: Library name: ENU55100B6G38_D3_neuro2091_S20_R1_001


title: ENU55100B6G39_D4_neuro2091_S20_R1_001

description: Library name: ENU55100B6G39_D4_neuro2091_S20_R1_001


title: ENU55100B6G37_D2_neuro2091_S20_R1_001

description: Library name: ENU55100B6G37_D2_neuro2091_S20_R1_001


title: ENU55100B6G36_D1_neuro2091_S20_R1_001

description: Library name: ENU55100B6G36_D1_neuro2091_S20_R1_001


title: ENU55100B6G327_D12_neuro2091_S20_R1_001

description: Library name: ENU55100B6G327_D12_neuro2091_S20_R1_001


title: ENU55100B6G326_D7_neuro2091_S20_R1_001

description: Library name: ENU55100B6G326_D7_neuro2091_S20_R1_001


title: ENU55100B6G325_D6_neuro2091_S20_R1_001

description: Library name: ENU55100B6G325_D6_neuro2091_S20_R1_001


title: ENU55100B6G324_D11_neuro2091_S20_R1_001

description: Library name: ENU55100B6G324_D11_neuro2091_S20_R1_001


title: ENU55116B6G37_A8_neuro2088_S1_R1_001

description: Library name: ENU55116B6G37_A8_neuro2088_S1_R1_001


title: ENU55100B6G316_D9_neuro2091_S20_R1_001

description: Library name: ENU55100B6G316_D9_neuro2091_S20_R1_001


title: ENU55100B6G323_D10_neuro2091_S20_R1_001

description: Library name: ENU55100B6G323_D10_neuro2091_S20_R1_001


title: ENU55100B6G315_D8_neuro2091_S20_R1_001

description: Library name: ENU55100B6G315_D8_neuro2091_S20_R1_001


title: ENU55098B6G36_G5_neuro2095_S55_R1_001

description: Library name: ENU55098B6G36_G5_neuro2095_S55_R1_001


title: ENU55098B6G35_G4_neuro2095_S55_R1_001

description: Library name: ENU55098B6G35_G4_neuro2095_S55_R1_001


title: ENU55098B6G34_G3_neuro2095_S55_R1_001

description: Library name: ENU55098B6G34_G3_neuro2095_S55_R1_001


title: ENU55100B6G314_D5_neuro2091_S20_R1_001

description: Library name: ENU55100B6G314_D5_neuro2091_S20_R1_001


title: ENU55173B6G36_A3_neuro2092_S25_R1_001

description: Library name: ENU55173B6G36_A3_neuro2092_S25_R1_001


title: ENU55278B6G310_A9_neuro2094_S41_R1_001

description: Library name: ENU55278B6G310_A9_neuro2094_S41_R1_001


title: ENU55278B6G32_A2_neuro2094_S41_R1_001

description: Library name: ENU55278B6G32_A2_neuro2094_S41_R1_001


title: ENU55098B6G333_G12_neuro2095_S55_R1_001

description: Library name: ENU55098B6G333_G12_neuro2095_S55_R1_001


title: ENU55098B6G332_G11_neuro2095_S55_R1_001

description: Library name: ENU55098B6G332_G11_neuro2095_S55_R1_001


title: ENU55098B6G330_G9_neuro2095_S55_R1_001

description: Library name: ENU55098B6G330_G9_neuro2095_S55_R1_001


title: ENU55098B6G310_G6_neuro2095_S55_R1_001

description: Library name: ENU55098B6G310_G6_neuro2095_S55_R1_001


title: ENU55098B6G331_G10_neuro2095_S55_R1_001

description: Library name: ENU55098B6G331_G10_neuro2095_S55_R1_001


title: ENU55092B6G37_B9_neuro2091_S18_R1_001

description: Library name: ENU55092B6G37_B9_neuro2091_S18_R1_001


title: ENU55098B6G329_G8_neuro2095_S55_R1_001

description: Library name: ENU55098B6G329_G8_neuro2095_S55_R1_001


title: ENU55098B6G32_G1_neuro2095_S55_R1_001

description: Library name: ENU55098B6G32_G1_neuro2095_S55_R1_001


title: ENU55098B6G328_G7_neuro2095_S55_R1_001

description: Library name: ENU55098B6G328_G7_neuro2095_S55_R1_001


title: ENU55092B6G39_B3_neuro2091_S18_R1_001

description: Library name: ENU55092B6G39_B3_neuro2091_S18_R1_001


title: ENU55092B6G34_B6_neuro2091_S18_R1_001

description: Library name: ENU55092B6G34_B6_neuro2091_S18_R1_001


title: ENU55092B6G32_B2_neuro2091_S18_R1_001

description: Library name: ENU55092B6G32_B2_neuro2091_S18_R1_001


title: ENU55092B6G36_B8_neuro2091_S18_R1_001

description: Library name: ENU55092B6G36_B8_neuro2091_S18_R1_001


title: ENU55092B6G31_B1_neuro2091_S18_R1_001

description: Library name: ENU55092B6G31_B1_neuro2091_S18_R1_001


title: ENU55092B6G312_B4_neuro2091_S18_R1_001

description: Library name: ENU55092B6G312_B4_neuro2091_S18_R1_001


title: ENU55092B6G314_B12_neuro2091_S18_R1_001

description: Library name: ENU55092B6G314_B12_neuro2091_S18_R1_001


title: ENU55092B6G313_B5_neuro2091_S18_R1_001

description: Library name: ENU55092B6G313_B5_neuro2091_S18_R1_001


title: ENU55092B6G311_B11_neuro2091_S18_R1_001

description: Library name: ENU55092B6G311_B11_neuro2091_S18_R1_001


title: ENU55092B6G310_B10_neuro2091_S18_R1_001

description: Library name: ENU55092B6G310_B10_neuro2091_S18_R1_001


title: ENU55083B6G39_A8_neuro2093_S33_R1_001

description: Library name: ENU55083B6G39_A8_neuro2093_S33_R1_001


title: ENU55083B6G38_A7_neuro2093_S33_R1_001

description: Library name: ENU55083B6G38_A7_neuro2093_S33_R1_001


title: ENU55083B6G37_A6_neuro2093_S33_R1_001

description: Library name: ENU55083B6G37_A6_neuro2093_S33_R1_001


title: ENU55130B6G33_F1_neuro2089_S14_R1_001

description: Library name: ENU55130B6G33_F1_neuro2089_S14_R1_001


title: ENU55083B6G36_A5_neuro2093_S33_R1_001

description: Library name: ENU55083B6G36_A5_neuro2093_S33_R1_001


title: ENU55083B6G32_A2_neuro2093_S33_R1_001

description: Library name: ENU55083B6G32_A2_neuro2093_S33_R1_001


title: ENU55083B6G31_A1_neuro2093_S33_R1_001

description: Library name: ENU55083B6G31_A1_neuro2093_S33_R1_001


title: ENU55083B6G313_A12_neuro2093_S33_R1_001

description: Library name: ENU55083B6G313_A12_neuro2093_S33_R1_001


title: ENU55083B6G34_A4_neuro2093_S33_R1_001

description: Library name: ENU55083B6G34_A4_neuro2093_S33_R1_001


title: ENU55083B6G33_A3_neuro2093_S33_R1_001

description: Library name: ENU55083B6G33_A3_neuro2093_S33_R1_001


title: ENU55254B6G313_D11_neuro2094_S44_R1_001

description: Library name: ENU55254B6G313_D11_neuro2094_S44_R1_001


title: ENU55083B6G310_A9_neuro2093_S33_R1_001

description: Library name: ENU55083B6G310_A9_neuro2093_S33_R1_001


title: ENU55081B6G37_F5_neuro2099_S86_R1_001

description: Library name: ENU55081B6G37_F5_neuro2099_S86_R1_001


title: ENU55083B6G311_A10_neuro2093_S33_R1_001

description: Library name: ENU55083B6G311_A10_neuro2093_S33_R1_001


title: ENU55081B6G31_F1_neuro2099_S86_R1_001

description: Library name: ENU55081B6G31_F1_neuro2099_S86_R1_001


title: ENU55081B6G36_F4_neuro2099_S86_R1_001

description: Library name: ENU55081B6G36_F4_neuro2099_S86_R1_001


title: ENU55081B6G33_F3_neuro2099_S86_R1_001

description: Library name: ENU55081B6G33_F3_neuro2099_S86_R1_001


title: ENU55081B6G32_F2_neuro2099_S86_R1_001

description: Library name: ENU55081B6G32_F2_neuro2099_S86_R1_001


title: ENU55081B6G318_F11_neuro2099_S86_R1_001

description: Library name: ENU55081B6G318_F11_neuro2099_S86_R1_001


title: ENU55081B6G317_F10_neuro2099_S86_R1_001

description: Library name: ENU55081B6G317_F10_neuro2099_S86_R1_001


title: ENU55081B6G319_F12_neuro2099_S86_R1_001

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