title: ENU54004B6G316_C11_neuro2025_S91_R1_001

description: Library name: ENU54004B6G316_C11_neuro2025_S91_R1_001


title: ENU54004B6G36_C9_neuro2025_S91_R1_001

status: Public on Apr 11 2026

type: SRA

city: South San Francisco

first: Mitchell

last: Rezzonico

state: CA

address: 1 DNA Way

country: USA

institute: Genentech

description: Library name: ENU54004B6G36_C9_neuro2025_S91_R1_001

library_source: transcriptomic

data_processing:
RNA-seq reads were aligned to the mouse reference genome GRCm38 using GSNAP 74. Only uniquely mapping reads were carried forward to downstream analysis. Sample quality was further assessed on a per-plate basis based on total read counts, percentage of reads mapping to the reference genome, and percentage of reads mapping to exons. Reads predominantly mapped to transcript 3’ ends, as expected from the library preparation method.
Assembly: GRCm38

molecule: total RNA

organism: Mus musculus

source_name: Whole brain

growth_protocol: Founder C57BL/6NCrl males were treated with 3 x 100 mg/kg ENU (Sigma) and mutations bred to homozygosity in three generations. Generation 3 mice were collected usually between 8-12 weeks old, and an ear notch for DNA and brain samples were collected. Typically, 12 siblings from each pedigree were screened.

extract_protocol:
To homogenize the tissue, RNAlater was removed, and a 5 mm stainless steel bead (Qiagen) and 1.6 mL of Qiazol (Qiagen) was added. The tissue was homogenized using a Tissue Lyser II (Qiagen) for at least 30 minutes until a homogenous solution was confirmed. 400 µL of the homogenate solution was transferred into a new tube and allowed to sit for 5 minutes before adding 100 µL of chloroform (Sigma). The homogenate-chloroform mixture was shaken vigorously, allowed to stand for 2 minutes before centrifugation for 5 minutes at 4°C and 1000 x g. The top clear layer was removed and stored at -80 °C. For RNA extraction, 120 µL was transferred into a QIAgen S-block and processed on the QIAcube using the RNeasy 96 QIAcube HT Kit.
cDNA fragment libraries were constructed using the SMART-Seq® v4 3’ DE Kit (Takara Bio) following manufacturer’s recommendations, with minor modifications. Total RNA samples were processed in batches of 1152, distributed across twelve 96-well plates. For each sample, 4 ng total RNA was used as input for cDNA synthesis, then amplified for 8 cycles, with each sample in a row receiving a unique in-line index. Amplified cDNAs within a row were then combined into a 12-plex pool, cleaned up with Ampure XP beads (Beckman Coulter) and quantified using the Qubit HS DNA assay kit (Thermo Fisher Scientific). For each of the 96 cDNA pools generated from 12 plates, 400 pg were carried into Nextera XT library preparations (Illumina Inc.), each assigned with one of the 96 unique combinations of Forward and Reverse HT Index Primers included in the SMART-Seq® v4 3’ DE kit. After Ampure XP clean up and Qubit quantification, the 96 pools were combined in an equimolar manner to generate a 1152-plex Illumina library.  This library was concentrated with Ampure XP beads, quantified with Qubit and assessed on the TapeStation with the High Sensitivity D1000 assay (Agilent Technologies).

treatment_protocol: Mice were euthanized by cervical dislocation, then whole brains were collected. Each cerebellum was removed and the remaining brain matter was transferred to RNAlater and stored at 4C for up to 5 days.

tag: tissue

value: Whole brain

tag: partofsubset

value: FALSE


title: ENU54004B6G313_C4_neuro2025_S91_R1_001

description: Library name: ENU54004B6G313_C4_neuro2025_S91_R1_001


title: ENU54004B6G33_C6_neuro2025_S91_R1_001

description: Library name: ENU54004B6G33_C6_neuro2025_S91_R1_001


description: Library name: ENU22Ms4a7B6G2RN312_D9_neuro2121_S28_R1_001


title: ENU54004B6G312_C3_neuro2025_S91_R1_001

description: Library name: ENU54004B6G312_C3_neuro2025_S91_R1_001


title: ENU54001B6G35_B5_neuro2025_S90_R1_001

description: Library name: ENU54001B6G35_B5_neuro2025_S90_R1_001


title: ENU54001B6G36_B6_neuro2025_S90_R1_001

description: Library name: ENU54001B6G36_B6_neuro2025_S90_R1_001


title: ENU54001B6G39_B7_neuro2025_S90_R1_001

description: Library name: ENU54001B6G39_B7_neuro2025_S90_R1_001


title: ENU54004B6G314_C5_neuro2025_S91_R1_001

description: Library name: ENU54004B6G314_C5_neuro2025_S91_R1_001


title: ENU54001B6G32_B2_neuro2025_S90_R1_001

description: Library name: ENU54001B6G32_B2_neuro2025_S90_R1_001


title: ENU54001B6G31_B1_neuro2025_S90_R1_001

description: Library name: ENU54001B6G31_B1_neuro2025_S90_R1_001


title: ENU54004B6G32_C2_neuro2025_S91_R1_001

description: Library name: ENU54004B6G32_C2_neuro2025_S91_R1_001


title: ENU54001B6G311_B9_neuro2025_S90_R1_001

description: Library name: ENU54001B6G311_B9_neuro2025_S90_R1_001


title: ENU54001B6G33_B3_neuro2025_S90_R1_001

description: Library name: ENU54001B6G33_B3_neuro2025_S90_R1_001


title: ENU54001B6G313_B11_neuro2025_S90_R1_001

description: Library name: ENU54001B6G313_B11_neuro2025_S90_R1_001


title: ENU54001B6G34_B4_neuro2025_S90_R1_001

description: Library name: ENU54001B6G34_B4_neuro2025_S90_R1_001


title: ENU54001B6G310_B8_neuro2025_S90_R1_001

description: Library name: ENU54001B6G310_B8_neuro2025_S90_R1_001


title: ENU53456B6G39_A4_neuro2025_S89_R1_001

description: Library name: ENU53456B6G39_A4_neuro2025_S89_R1_001

value: TRUE

value: Kat6a E1782K


title: ENU54001B6G312_B10_neuro2025_S90_R1_001

description: Library name: ENU54001B6G312_B10_neuro2025_S90_R1_001


title: ENU54001B6G314_B12_neuro2025_S90_R1_001

description: Library name: ENU54001B6G314_B12_neuro2025_S90_R1_001


title: ENU53456B6G34_A7_neuro2025_S89_R1_001

description: Library name: ENU53456B6G34_A7_neuro2025_S89_R1_001


title: ENU53456B6G38_A3_neuro2025_S89_R1_001

description: Library name: ENU53456B6G38_A3_neuro2025_S89_R1_001

value: MG density

value: m/m


title: ENU53456B6G33_A6_neuro2025_S89_R1_001

description: Library name: ENU53456B6G33_A6_neuro2025_S89_R1_001


title: ENU53456B6G35_A8_neuro2025_S89_R1_001

description: Library name: ENU53456B6G35_A8_neuro2025_S89_R1_001


title: ENU53456B6G37_A2_neuro2025_S89_R1_001

description: Library name: ENU53456B6G37_A2_neuro2025_S89_R1_001


title: ENU53456B6G36_A9_neuro2025_S89_R1_001

description: Library name: ENU53456B6G36_A9_neuro2025_S89_R1_001


title: ENU53456B6G31_A1_neuro2025_S89_R1_001

description: Library name: ENU53456B6G31_A1_neuro2025_S89_R1_001


title: ENU53456B6G312_A12_neuro2025_S89_R1_001

description: Library name: ENU53456B6G312_A12_neuro2025_S89_R1_001


title: ENU53456B6G32_A5_neuro2025_S89_R1_001

description: Library name: ENU53456B6G32_A5_neuro2025_S89_R1_001


title: ENU53441B6G319_H11_neuro2018_S48_R1_001

description: Library name: ENU53441B6G319_H11_neuro2018_S48_R1_001


title: ENU53441B6G36_H2_neuro2018_S48_R1_001

description: Library name: ENU53441B6G36_H2_neuro2018_S48_R1_001


title: ENU53441B6G35_H1_neuro2018_S48_R1_001

description: Library name: ENU53441B6G35_H1_neuro2018_S48_R1_001


title: ENU53441B6G38_H4_neuro2018_S48_R1_001

description: Library name: ENU53441B6G38_H4_neuro2018_S48_R1_001


title: ENU53441B6G37_H3_neuro2018_S48_R1_001

description: Library name: ENU53441B6G37_H3_neuro2018_S48_R1_001


title: ENU53441B6G317_H9_neuro2018_S48_R1_001

description: Library name: ENU53441B6G317_H9_neuro2018_S48_R1_001


title: ENU53440B6G39_H5_neuro2024_S88_R1_001

description: Library name: ENU53440B6G39_H5_neuro2024_S88_R1_001


title: ENU53441B6G39_H5_neuro2018_S48_R1_001

description: Library name: ENU53441B6G39_H5_neuro2018_S48_R1_001


title: ENU53441B6G318_H10_neuro2018_S48_R1_001

description: Library name: ENU53441B6G318_H10_neuro2018_S48_R1_001


title: ENU53456B6G311_A11_neuro2025_S89_R1_001

description: Library name: ENU53456B6G311_A11_neuro2025_S89_R1_001


title: ENU53440B6G36_H8_neuro2024_S88_R1_001

description: Library name: ENU53440B6G36_H8_neuro2024_S88_R1_001


title: ENU53441B6G320_H12_neuro2018_S48_R1_001

description: Library name: ENU53441B6G320_H12_neuro2018_S48_R1_001


title: ENU53440B6G37_H9_neuro2024_S88_R1_001

description: Library name: ENU53440B6G37_H9_neuro2024_S88_R1_001


title: ENU53441B6G310_H6_neuro2018_S48_R1_001

description: Library name: ENU53441B6G310_H6_neuro2018_S48_R1_001


title: ENU53440B6G38_H4_neuro2024_S88_R1_001

description: Library name: ENU53440B6G38_H4_neuro2024_S88_R1_001


title: ENU53441B6G315_H7_neuro2018_S48_R1_001

description: Library name: ENU53441B6G315_H7_neuro2018_S48_R1_001


title: ENU53440B6G33_H3_neuro2024_S88_R1_001

description: Library name: ENU53440B6G33_H3_neuro2024_S88_R1_001


title: ENU53440B6G32_H2_neuro2024_S88_R1_001

description: Library name: ENU53440B6G32_H2_neuro2024_S88_R1_001


title: ENU53456B6G310_A10_neuro2025_S89_R1_001

description: Library name: ENU53456B6G310_A10_neuro2025_S89_R1_001


title: ENU53440B6G34_H6_neuro2024_S88_R1_001

description: Library name: ENU53440B6G34_H6_neuro2024_S88_R1_001


title: ENU53440B6G35_H7_neuro2024_S88_R1_001

description: Library name: ENU53440B6G35_H7_neuro2024_S88_R1_001


title: ENU53440B6G311_H11_neuro2024_S88_R1_001

description: Library name: ENU53440B6G311_H11_neuro2024_S88_R1_001


title: ENU53436B6G39_F9_neuro2019_S54_R1_001

description: Library name: ENU53436B6G39_F9_neuro2019_S54_R1_001


title: ENU53440B6G31_H1_neuro2024_S88_R1_001

description: Library name: ENU53440B6G31_H1_neuro2024_S88_R1_001


title: ENU53440B6G310_H10_neuro2024_S88_R1_001

description: Library name: ENU53440B6G310_H10_neuro2024_S88_R1_001


title: ENU53440B6G312_H12_neuro2024_S88_R1_001

description: Library name: ENU53440B6G312_H12_neuro2024_S88_R1_001


title: ENU53441B6G316_H8_neuro2018_S48_R1_001

description: Library name: ENU53441B6G316_H8_neuro2018_S48_R1_001


title: ENU53436B6G38_F8_neuro2019_S54_R1_001

description: Library name: ENU53436B6G38_F8_neuro2019_S54_R1_001


title: ENU53436B6G37_F7_neuro2019_S54_R1_001

description: Library name: ENU53436B6G37_F7_neuro2019_S54_R1_001


title: ENU53436B6G35_F5_neuro2019_S54_R1_001

description: Library name: ENU53436B6G35_F5_neuro2019_S54_R1_001


title: ENU53436B6G34_F4_neuro2019_S54_R1_001

description: Library name: ENU53436B6G34_F4_neuro2019_S54_R1_001


title: ENU53436B6G36_F6_neuro2019_S54_R1_001

description: Library name: ENU53436B6G36_F6_neuro2019_S54_R1_001


title: ENU53436B6G312_F12_neuro2019_S54_R1_001

description: Library name: ENU53436B6G312_F12_neuro2019_S54_R1_001


title: ENU53436B6G31_F1_neuro2019_S54_R1_001

description: Library name: ENU53436B6G31_F1_neuro2019_S54_R1_001


title: ENU53436B6G32_F2_neuro2019_S54_R1_001

description: Library name: ENU53436B6G32_F2_neuro2019_S54_R1_001


title: ENU53436B6G310_F10_neuro2019_S54_R1_001

description: Library name: ENU53436B6G310_F10_neuro2019_S54_R1_001


title: ENU53432B6G37_G12_neuro2018_S47_R1_001

description: Library name: ENU53432B6G37_G12_neuro2018_S47_R1_001


title: ENU53432B6G34_G7_neuro2018_S47_R1_001

description: Library name: ENU53432B6G34_G7_neuro2018_S47_R1_001


title: ENU53432B6G39_G3_neuro2018_S47_R1_001

description: Library name: ENU53432B6G39_G3_neuro2018_S47_R1_001


title: ENU53432B6G38_G2_neuro2018_S47_R1_001

description: Library name: ENU53432B6G38_G2_neuro2018_S47_R1_001


title: ENU53432B6G33_G6_neuro2018_S47_R1_001

description: Library name: ENU53432B6G33_G6_neuro2018_S47_R1_001


title: ENU53432B6G310_G4_neuro2018_S47_R1_001

description: Library name: ENU53432B6G310_G4_neuro2018_S47_R1_001


title: ENU53432B6G311_G8_neuro2018_S47_R1_001

description: Library name: ENU53432B6G311_G8_neuro2018_S47_R1_001


title: ENU53432B6G312_G9_neuro2018_S47_R1_001

description: Library name: ENU53432B6G312_G9_neuro2018_S47_R1_001


title: ENU53429B6G39_E2_neuro2019_S53_R1_001

description: Library name: ENU53429B6G39_E2_neuro2019_S53_R1_001


title: ENU53429B6G38_E1_neuro2019_S53_R1_001

description: Library name: ENU53429B6G38_E1_neuro2019_S53_R1_001


title: ENU53432B6G32_G5_neuro2018_S47_R1_001

description: Library name: ENU53432B6G32_G5_neuro2018_S47_R1_001


title: ENU53429B6G317_E10_neuro2019_S53_R1_001

description: Library name: ENU53429B6G317_E10_neuro2019_S53_R1_001


title: ENU53429B6G319_E12_neuro2019_S53_R1_001

description: Library name: ENU53429B6G319_E12_neuro2019_S53_R1_001


title: ENU53429B6G318_E11_neuro2019_S53_R1_001

description: Library name: ENU53429B6G318_E11_neuro2019_S53_R1_001


title: ENU53432B6G35_G10_neuro2018_S47_R1_001

description: Library name: ENU53432B6G35_G10_neuro2018_S47_R1_001


title: ENU53436B6G33_F3_neuro2019_S54_R1_001

description: Library name: ENU53436B6G33_F3_neuro2019_S54_R1_001


title: ENU53436B6G311_F11_neuro2019_S54_R1_001

description: Library name: ENU53436B6G311_F11_neuro2019_S54_R1_001


title: ENU53429B6G316_E9_neuro2019_S53_R1_001

description: Library name: ENU53429B6G316_E9_neuro2019_S53_R1_001


title: ENU53429B6G314_E7_neuro2019_S53_R1_001

description: Library name: ENU53429B6G314_E7_neuro2019_S53_R1_001


title: ENU53429B6G312_E5_neuro2019_S53_R1_001

description: Library name: ENU53429B6G312_E5_neuro2019_S53_R1_001


title: ENU53432B6G36_G11_neuro2018_S47_R1_001

description: Library name: ENU53432B6G36_G11_neuro2018_S47_R1_001


title: ENU53429B6G315_E8_neuro2019_S53_R1_001

description: Library name: ENU53429B6G315_E8_neuro2019_S53_R1_001


title: ENU53429B6G313_E6_neuro2019_S53_R1_001

description: Library name: ENU53429B6G313_E6_neuro2019_S53_R1_001


title: ENU53420B6G38_E8_neuro2023_S77_R1_001

description: Library name: ENU53420B6G38_E8_neuro2023_S77_R1_001


title: ENU53432B6G31_G1_neuro2018_S47_R1_001

description: Library name: ENU53432B6G31_G1_neuro2018_S47_R1_001


title: ENU53429B6G311_E4_neuro2019_S53_R1_001

description: Library name: ENU53429B6G311_E4_neuro2019_S53_R1_001


title: ENU53420B6G37_E5_neuro2023_S77_R1_001

description: Library name: ENU53420B6G37_E5_neuro2023_S77_R1_001


title: ENU53420B6G36_E4_neuro2023_S77_R1_001

description: Library name: ENU53420B6G36_E4_neuro2023_S77_R1_001


title: ENU53420B6G39_E9_neuro2023_S77_R1_001

description: Library name: ENU53420B6G39_E9_neuro2023_S77_R1_001


title: ENU53429B6G310_E3_neuro2019_S53_R1_001

description: Library name: ENU53429B6G310_E3_neuro2019_S53_R1_001


title: ENU53420B6G33_E3_neuro2023_S77_R1_001

description: Library name: ENU53420B6G33_E3_neuro2023_S77_R1_001


title: ENU53420B6G312_E12_neuro2023_S77_R1_001

description: Library name: ENU53420B6G312_E12_neuro2023_S77_R1_001


title: ENU53420B6G31_E1_neuro2023_S77_R1_001

description: Library name: ENU53420B6G31_E1_neuro2023_S77_R1_001


title: ENU53420B6G311_E11_neuro2023_S77_R1_001

description: Library name: ENU53420B6G311_E11_neuro2023_S77_R1_001


title: ENU53420B6G32_E2_neuro2023_S77_R1_001

description: Library name: ENU53420B6G32_E2_neuro2023_S77_R1_001


title: ENU53419B6G36_G9_neuro2024_S87_R1_001

description: Library name: ENU53419B6G36_G9_neuro2024_S87_R1_001


title: ENU53420B6G310_E10_neuro2023_S77_R1_001

description: Library name: ENU53420B6G310_E10_neuro2023_S77_R1_001


title: ENU53420B6G34_E6_neuro2023_S77_R1_001

description: Library name: ENU53420B6G34_E6_neuro2023_S77_R1_001


title: ENU53420B6G35_E7_neuro2023_S77_R1_001

description: Library name: ENU53420B6G35_E7_neuro2023_S77_R1_001


title: ENU53419B6G33_G6_neuro2024_S87_R1_001

description: Library name: ENU53419B6G33_G6_neuro2024_S87_R1_001


title: ENU53419B6G31_G1_neuro2024_S87_R1_001

description: Library name: ENU53419B6G31_G1_neuro2024_S87_R1_001


title: ENU53419B6G34_G7_neuro2024_S87_R1_001

description: Library name: ENU53419B6G34_G7_neuro2024_S87_R1_001


title: ENU53419B6G39_G4_neuro2024_S87_R1_001

description: Library name: ENU53419B6G39_G4_neuro2024_S87_R1_001


title: ENU53419B6G313_G12_neuro2024_S87_R1_001

description: Library name: ENU53419B6G313_G12_neuro2024_S87_R1_001


title: ENU53419B6G311_G10_neuro2024_S87_R1_001

description: Library name: ENU53419B6G311_G10_neuro2024_S87_R1_001


title: ENU53419B6G32_G2_neuro2024_S87_R1_001

description: Library name: ENU53419B6G32_G2_neuro2024_S87_R1_001


title: ENU53414B6G36_D6_neuro2023_S76_R1_001

description: Library name: ENU53414B6G36_D6_neuro2023_S76_R1_001


title: ENU53419B6G310_G5_neuro2024_S87_R1_001

description: Library name: ENU53419B6G310_G5_neuro2024_S87_R1_001


title: ENU53419B6G312_G11_neuro2024_S87_R1_001

description: Library name: ENU53419B6G312_G11_neuro2024_S87_R1_001


title: ENU53419B6G38_G3_neuro2024_S87_R1_001

description: Library name: ENU53419B6G38_G3_neuro2024_S87_R1_001


title: ENU53419B6G35_G8_neuro2024_S87_R1_001

description: Library name: ENU53419B6G35_G8_neuro2024_S87_R1_001


title: ENU53414B6G33_D3_neuro2023_S76_R1_001

description: Library name: ENU53414B6G33_D3_neuro2023_S76_R1_001


title: ENU53414B6G35_D5_neuro2023_S76_R1_001

description: Library name: ENU53414B6G35_D5_neuro2023_S76_R1_001


title: ENU53414B6G34_D4_neuro2023_S76_R1_001

description: Library name: ENU53414B6G34_D4_neuro2023_S76_R1_001


title: ENU53414B6G32_D2_neuro2023_S76_R1_001

description: Library name: ENU53414B6G32_D2_neuro2023_S76_R1_001


title: ENU53414B6G313_D10_neuro2023_S76_R1_001

description: Library name: ENU53414B6G313_D10_neuro2023_S76_R1_001


title: ENU53414B6G315_D12_neuro2023_S76_R1_001

description: Library name: ENU53414B6G315_D12_neuro2023_S76_R1_001


title: ENU53414B6G314_D11_neuro2023_S76_R1_001

description: Library name: ENU53414B6G314_D11_neuro2023_S76_R1_001


title: ENU53414B6G31_D1_neuro2023_S76_R1_001

description: Library name: ENU53414B6G31_D1_neuro2023_S76_R1_001


title: ENU53414B6G312_D9_neuro2023_S76_R1_001

description: Library name: ENU53414B6G312_D9_neuro2023_S76_R1_001


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description: Library name: ENU53414B6G311_D8_neuro2023_S76_R1_001


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