title: ENU55148B6G38_B8_neuro2129_S66_R1_001

description: Library name: ENU55148B6G38_B8_neuro2129_S66_R1_001


title: ENU55148B6G310_B9_neuro2129_S66_R1_001

status: Public on Apr 11 2026

type: SRA

city: South San Francisco

first: Mitchell

last: Rezzonico

state: CA

address: 1 DNA Way

country: USA

institute: Genentech

description: Library name: ENU55148B6G310_B9_neuro2129_S66_R1_001

library_source: transcriptomic

data_processing:
RNA-seq reads were aligned to the mouse reference genome GRCm38 using GSNAP 74. Only uniquely mapping reads were carried forward to downstream analysis. Sample quality was further assessed on a per-plate basis based on total read counts, percentage of reads mapping to the reference genome, and percentage of reads mapping to exons. Reads predominantly mapped to transcript 3’ ends, as expected from the library preparation method.
Assembly: GRCm38

molecule: total RNA

organism: Mus musculus

source_name: Whole brain

growth_protocol: Founder C57BL/6NCrl males were treated with 3 x 100 mg/kg ENU (Sigma) and mutations bred to homozygosity in three generations. Generation 3 mice were collected usually between 8-12 weeks old, and an ear notch for DNA and brain samples were collected. Typically, 12 siblings from each pedigree were screened.

extract_protocol:
To homogenize the tissue, RNAlater was removed, and a 5 mm stainless steel bead (Qiagen) and 1.6 mL of Qiazol (Qiagen) was added. The tissue was homogenized using a Tissue Lyser II (Qiagen) for at least 30 minutes until a homogenous solution was confirmed. 400 µL of the homogenate solution was transferred into a new tube and allowed to sit for 5 minutes before adding 100 µL of chloroform (Sigma). The homogenate-chloroform mixture was shaken vigorously, allowed to stand for 2 minutes before centrifugation for 5 minutes at 4°C and 1000 x g. The top clear layer was removed and stored at -80 °C. For RNA extraction, 120 µL was transferred into a QIAgen S-block and processed on the QIAcube using the RNeasy 96 QIAcube HT Kit.
cDNA fragment libraries were constructed using the SMART-Seq® v4 3’ DE Kit (Takara Bio) following manufacturer’s recommendations, with minor modifications. Total RNA samples were processed in batches of 1152, distributed across twelve 96-well plates. For each sample, 4 ng total RNA was used as input for cDNA synthesis, then amplified for 8 cycles, with each sample in a row receiving a unique in-line index. Amplified cDNAs within a row were then combined into a 12-plex pool, cleaned up with Ampure XP beads (Beckman Coulter) and quantified using the Qubit HS DNA assay kit (Thermo Fisher Scientific). For each of the 96 cDNA pools generated from 12 plates, 400 pg were carried into Nextera XT library preparations (Illumina Inc.), each assigned with one of the 96 unique combinations of Forward and Reverse HT Index Primers included in the SMART-Seq® v4 3’ DE kit. After Ampure XP clean up and Qubit quantification, the 96 pools were combined in an equimolar manner to generate a 1152-plex Illumina library.  This library was concentrated with Ampure XP beads, quantified with Qubit and assessed on the TapeStation with the High Sensitivity D1000 assay (Agilent Technologies).

treatment_protocol: Mice were euthanized by cervical dislocation, then whole brains were collected. Each cerebellum was removed and the remaining brain matter was transferred to RNAlater and stored at 4C for up to 5 days.

tag: tissue

value: Whole brain

tag: partofsubset

value: FALSE


title: ENU55170B6G310_C9_neuro2112_S27_R1_001

description: Library name: ENU55170B6G310_C9_neuro2112_S27_R1_001


title: ENU55148B6G36_B6_neuro2129_S66_R1_001

description: Library name: ENU55148B6G36_B6_neuro2129_S66_R1_001


title: ENU55109B6G39_B8_neuro2112_S26_R1_001

description: Library name: ENU55109B6G39_B8_neuro2112_S26_R1_001


title: ENU55148B6G31_B1_neuro2129_S66_R1_001

description: Library name: ENU55148B6G31_B1_neuro2129_S66_R1_001


title: ENU55109B6G38_B7_neuro2112_S26_R1_001

description: Library name: ENU55109B6G38_B7_neuro2112_S26_R1_001


title: ENU55148B6G32_B4_neuro2129_S66_R1_001

description: Library name: ENU55148B6G32_B4_neuro2129_S66_R1_001


title: ENU55109B6G35_B5_neuro2112_S26_R1_001

description: Library name: ENU55109B6G35_B5_neuro2112_S26_R1_001


title: ENU55109B6G36_B6_neuro2112_S26_R1_001

description: Library name: ENU55109B6G36_B6_neuro2112_S26_R1_001


title: ENU55109B6G32_B2_neuro2112_S26_R1_001

description: Library name: ENU55109B6G32_B2_neuro2112_S26_R1_001


title: ENU55109B6G34_B4_neuro2112_S26_R1_001

description: Library name: ENU55109B6G34_B4_neuro2112_S26_R1_001


title: ENU55109B6G324_B12_neuro2112_S26_R1_001

description: Library name: ENU55109B6G324_B12_neuro2112_S26_R1_001


title: ENU55109B6G33_B3_neuro2112_S26_R1_001

description: Library name: ENU55109B6G33_B3_neuro2112_S26_R1_001


title: ENU55109B6G315_B9_neuro2112_S26_R1_001

description: Library name: ENU55109B6G315_B9_neuro2112_S26_R1_001


title: ENU55109B6G31_B1_neuro2112_S26_R1_001

description: Library name: ENU55109B6G31_B1_neuro2112_S26_R1_001


title: ENU55109B6G323_B11_neuro2112_S26_R1_001

description: Library name: ENU55109B6G323_B11_neuro2112_S26_R1_001


title: ENU53401B6G38_A6_neuro2112_S25_R1_001

description: Library name: ENU53401B6G38_A6_neuro2112_S25_R1_001


title: ENU53401B6G34_A4_neuro2112_S25_R1_001

description: Library name: ENU53401B6G34_A4_neuro2112_S25_R1_001


title: ENU53401B6G32_A2_neuro2112_S25_R1_001

description: Library name: ENU53401B6G32_A2_neuro2112_S25_R1_001


title: ENU53040B6G36_A12_neuro2112_S25_R1_001

description: Library name: ENU53040B6G36_A12_neuro2112_S25_R1_001


title: ENU53401B6G33_A3_neuro2112_S25_R1_001

description: Library name: ENU53401B6G33_A3_neuro2112_S25_R1_001


title: ENU53040B6G34_A10_neuro2112_S25_R1_001

description: Library name: ENU53040B6G34_A10_neuro2112_S25_R1_001


title: ENU53040B6G35_A11_neuro2112_S25_R1_001

description: Library name: ENU53040B6G35_A11_neuro2112_S25_R1_001


title: ENU53040B6G32_A8_neuro2112_S25_R1_001

description: Library name: ENU53040B6G32_A8_neuro2112_S25_R1_001


title: ENU53040B6G33_A9_neuro2112_S25_R1_001

description: Library name: ENU53040B6G33_A9_neuro2112_S25_R1_001


title: ENU53401B6G31_A1_neuro2112_S25_R1_001

description: Library name: ENU53401B6G31_A1_neuro2112_S25_R1_001


title: ENU53040B6G31_A7_neuro2112_S25_R1_001

description: Library name: ENU53040B6G31_A7_neuro2112_S25_R1_001


description: Library name: ENU43055B6G2RN223_F10_neuro2127_S14_R1_001

value: TRUE

value: Inpp5d W8X


description: Library name: ENU43055B6G2RN221_F8_neuro2127_S14_R1_001


description: Library name: ENU43055B6G2RN222_F9_neuro2127_S14_R1_001


description: Library name: ENU43055B6G2RN219_F6_neuro2127_S14_R1_001


description: Library name: ENU43055B6G2RN220_F7_neuro2127_S14_R1_001


description: Library name: ENU43055B6G2RN217_F4_neuro2127_S14_R1_001


description: Library name: ENU43055B6G2RN215_F2_neuro2127_S14_R1_001


description: Library name: ENU43055B6G2RN216_F3_neuro2127_S14_R1_001

value: m/m


description: Library name: ENU29013Inpp5dB6G2RN432_H6_neuro2127_S16_R1_001

value: Inpp5d S308X


description: Library name: ENU29013Inpp5dB6G2RN430_H4_neuro2127_S16_R1_001


description: Library name: ENU43055B6G2RN214_F1_neuro2127_S14_R1_001


description: Library name: ENU29013Inpp5dB6G2RN431_H5_neuro2127_S16_R1_001


description: Library name: ENU29013Inpp5dB6G2RN428_H3_neuro2127_S16_R1_001


description: Library name: ENU43055B6G2RN218_F5_neuro2127_S14_R1_001


description: Library name: ENU29013Inpp5dB6G2RN418_G10_neuro2127_S15_R1_001


description: Library name: ENU29013Inpp5dB6G2RN417_G9_neuro2127_S15_R1_001


description: Library name: ENU29013Inpp5dB6G2RN423_G12_neuro2127_S15_R1_001


description: Library name: ENU29013Inpp5dB6G2RN415_G7_neuro2127_S15_R1_001


description: Library name: ENU29013Inpp5dB6G2RN427_H2_neuro2127_S16_R1_001


description: Library name: ENU29013Inpp5dB6G2RN425_H1_neuro2127_S16_R1_001


description: Library name: ENU29013Inpp5dB6G2RN422_G11_neuro2127_S15_R1_001


description: Library name: ENU29013Inpp5dB6G2RN350_G4_neuro2127_S15_R1_001


title: ENU54295B6G39_G8_neuro2052_S79_R1_001

description: Library name: ENU54295B6G39_G8_neuro2052_S79_R1_001


description: Library name: ENU29013Inpp5dB6G2RN416_G8_neuro2127_S15_R1_001


description: Library name: ENU29013Inpp5dB6G2RN351_G5_neuro2127_S15_R1_001


description: Library name: ENU29013Inpp5dB6G2RN414_G6_neuro2127_S15_R1_001


title: ENU54295B6G38_G7_neuro2052_S79_R1_001

description: Library name: ENU54295B6G38_G7_neuro2052_S79_R1_001


title: ENU54295B6G35_G5_neuro2052_S79_R1_001

description: Library name: ENU54295B6G35_G5_neuro2052_S79_R1_001


title: ENU54295B6G312_G11_neuro2052_S79_R1_001

description: Library name: ENU54295B6G312_G11_neuro2052_S79_R1_001


title: ENU54295B6G313_G12_neuro2052_S79_R1_001

description: Library name: ENU54295B6G313_G12_neuro2052_S79_R1_001


title: ENU54295B6G34_G4_neuro2052_S79_R1_001

description: Library name: ENU54295B6G34_G4_neuro2052_S79_R1_001


title: ENU54295B6G31_G1_neuro2052_S79_R1_001

description: Library name: ENU54295B6G31_G1_neuro2052_S79_R1_001


title: ENU54295B6G311_G10_neuro2052_S79_R1_001

description: Library name: ENU54295B6G311_G10_neuro2052_S79_R1_001


title: ENU54295B6G310_G9_neuro2052_S79_R1_001

description: Library name: ENU54295B6G310_G9_neuro2052_S79_R1_001


title: ENU54282B6G35_F5_neuro2052_S78_R1_001

description: Library name: ENU54282B6G35_F5_neuro2052_S78_R1_001


title: ENU54295B6G33_G3_neuro2052_S79_R1_001

description: Library name: ENU54295B6G33_G3_neuro2052_S79_R1_001


title: ENU54282B6G34_F4_neuro2052_S78_R1_001

description: Library name: ENU54282B6G34_F4_neuro2052_S78_R1_001


title: ENU54295B6G36_G6_neuro2052_S79_R1_001

description: Library name: ENU54295B6G36_G6_neuro2052_S79_R1_001


title: ENU54282B6G36_F6_neuro2052_S78_R1_001

description: Library name: ENU54282B6G36_F6_neuro2052_S78_R1_001


title: ENU54295B6G32_G2_neuro2052_S79_R1_001

description: Library name: ENU54295B6G32_G2_neuro2052_S79_R1_001


title: ENU54282B6G33_F3_neuro2052_S78_R1_001

description: Library name: ENU54282B6G33_F3_neuro2052_S78_R1_001


title: ENU54282B6G319_F11_neuro2052_S78_R1_001

description: Library name: ENU54282B6G319_F11_neuro2052_S78_R1_001


title: ENU54282B6G32_F2_neuro2052_S78_R1_001

description: Library name: ENU54282B6G32_F2_neuro2052_S78_R1_001


title: ENU54282B6G31_F1_neuro2052_S78_R1_001

description: Library name: ENU54282B6G31_F1_neuro2052_S78_R1_001


title: ENU54282B6G320_F12_neuro2052_S78_R1_001

description: Library name: ENU54282B6G320_F12_neuro2052_S78_R1_001


title: ENU54282B6G316_F9_neuro2052_S78_R1_001

description: Library name: ENU54282B6G316_F9_neuro2052_S78_R1_001


title: ENU54282B6G315_F8_neuro2052_S78_R1_001

description: Library name: ENU54282B6G315_F8_neuro2052_S78_R1_001


title: ENU54274B6G33_A3_neuro2051_S65_R1_001

description: Library name: ENU54274B6G33_A3_neuro2051_S65_R1_001


title: ENU54274B6G37_A5_neuro2051_S65_R1_001

description: Library name: ENU54274B6G37_A5_neuro2051_S65_R1_001


title: ENU54274B6G38_A6_neuro2051_S65_R1_001

description: Library name: ENU54274B6G38_A6_neuro2051_S65_R1_001


title: ENU54282B6G317_F10_neuro2052_S78_R1_001

description: Library name: ENU54282B6G317_F10_neuro2052_S78_R1_001


title: ENU54274B6G32_A2_neuro2051_S65_R1_001

description: Library name: ENU54274B6G32_A2_neuro2051_S65_R1_001


title: ENU54274B6G31_A1_neuro2051_S65_R1_001

description: Library name: ENU54274B6G31_A1_neuro2051_S65_R1_001


title: ENU54274B6G316_A12_neuro2051_S65_R1_001

description: Library name: ENU54274B6G316_A12_neuro2051_S65_R1_001


title: ENU54274B6G315_A11_neuro2051_S65_R1_001

description: Library name: ENU54274B6G315_A11_neuro2051_S65_R1_001


title: ENU54282B6G314_F7_neuro2052_S78_R1_001

description: Library name: ENU54282B6G314_F7_neuro2052_S78_R1_001


title: ENU54274B6G314_A10_neuro2051_S65_R1_001

description: Library name: ENU54274B6G314_A10_neuro2051_S65_R1_001


title: ENU54274B6G313_A9_neuro2051_S65_R1_001

description: Library name: ENU54274B6G313_A9_neuro2051_S65_R1_001


title: ENU54274B6G312_A8_neuro2051_S65_R1_001

description: Library name: ENU54274B6G312_A8_neuro2051_S65_R1_001


title: ENU54231B6G39_D8_neuro2052_S76_R1_001

description: Library name: ENU54231B6G39_D8_neuro2052_S76_R1_001


title: ENU54231B6G36_D6_neuro2052_S76_R1_001

description: Library name: ENU54231B6G36_D6_neuro2052_S76_R1_001


title: ENU54231B6G38_D7_neuro2052_S76_R1_001

description: Library name: ENU54231B6G38_D7_neuro2052_S76_R1_001


title: ENU54274B6G34_A4_neuro2051_S65_R1_001

description: Library name: ENU54274B6G34_A4_neuro2051_S65_R1_001


title: ENU54231B6G34_D4_neuro2052_S76_R1_001

description: Library name: ENU54231B6G34_D4_neuro2052_S76_R1_001


title: ENU54231B6G33_D3_neuro2052_S76_R1_001

description: Library name: ENU54231B6G33_D3_neuro2052_S76_R1_001


title: ENU54231B6G315_D12_neuro2052_S76_R1_001

description: Library name: ENU54231B6G315_D12_neuro2052_S76_R1_001


title: ENU54231B6G32_D2_neuro2052_S76_R1_001

description: Library name: ENU54231B6G32_D2_neuro2052_S76_R1_001


title: ENU54231B6G314_D11_neuro2052_S76_R1_001

description: Library name: ENU54231B6G314_D11_neuro2052_S76_R1_001


title: ENU54231B6G311_D10_neuro2052_S76_R1_001

description: Library name: ENU54231B6G311_D10_neuro2052_S76_R1_001


title: ENU54214B6G39_C5_neuro2052_S75_R1_001

description: Library name: ENU54214B6G39_C5_neuro2052_S75_R1_001


title: ENU54214B6G37_C3_neuro2052_S75_R1_001

description: Library name: ENU54214B6G37_C3_neuro2052_S75_R1_001


title: ENU54231B6G310_D9_neuro2052_S76_R1_001

description: Library name: ENU54231B6G310_D9_neuro2052_S76_R1_001


title: ENU54214B6G38_C4_neuro2052_S75_R1_001

description: Library name: ENU54214B6G38_C4_neuro2052_S75_R1_001


title: ENU54214B6G317_C9_neuro2052_S75_R1_001

description: Library name: ENU54214B6G317_C9_neuro2052_S75_R1_001


title: ENU54231B6G31_D1_neuro2052_S76_R1_001

description: Library name: ENU54231B6G31_D1_neuro2052_S76_R1_001


title: ENU54214B6G36_C2_neuro2052_S75_R1_001

description: Library name: ENU54214B6G36_C2_neuro2052_S75_R1_001


title: ENU54214B6G35_C1_neuro2052_S75_R1_001

description: Library name: ENU54214B6G35_C1_neuro2052_S75_R1_001


title: ENU54214B6G322_C12_neuro2052_S75_R1_001

description: Library name: ENU54214B6G322_C12_neuro2052_S75_R1_001


title: ENU54214B6G321_C11_neuro2052_S75_R1_001

description: Library name: ENU54214B6G321_C11_neuro2052_S75_R1_001


title: ENU54214B6G316_C8_neuro2052_S75_R1_001

description: Library name: ENU54214B6G316_C8_neuro2052_S75_R1_001


title: ENU54200B6G37_B6_neuro2052_S74_R1_001

description: Library name: ENU54200B6G37_B6_neuro2052_S74_R1_001


title: ENU54214B6G315_C7_neuro2052_S75_R1_001

description: Library name: ENU54214B6G315_C7_neuro2052_S75_R1_001


title: ENU54214B6G320_C10_neuro2052_S75_R1_001

description: Library name: ENU54214B6G320_C10_neuro2052_S75_R1_001


title: ENU54200B6G35_B4_neuro2052_S74_R1_001

description: Library name: ENU54200B6G35_B4_neuro2052_S74_R1_001


title: ENU54214B6G310_C6_neuro2052_S75_R1_001

description: Library name: ENU54214B6G310_C6_neuro2052_S75_R1_001


title: ENU54200B6G33_B2_neuro2052_S74_R1_001

description: Library name: ENU54200B6G33_B2_neuro2052_S74_R1_001


title: ENU54200B6G36_B5_neuro2052_S74_R1_001

description: Library name: ENU54200B6G36_B5_neuro2052_S74_R1_001


title: ENU54200B6G34_B3_neuro2052_S74_R1_001

description: Library name: ENU54200B6G34_B3_neuro2052_S74_R1_001


title: ENU54200B6G31_B1_neuro2052_S74_R1_001

description: Library name: ENU54200B6G31_B1_neuro2052_S74_R1_001


title: ENU54200B6G313_B10_neuro2052_S74_R1_001

description: Library name: ENU54200B6G313_B10_neuro2052_S74_R1_001


title: ENU54200B6G315_B12_neuro2052_S74_R1_001

description: Library name: ENU54200B6G315_B12_neuro2052_S74_R1_001


title: ENU54200B6G314_B11_neuro2052_S74_R1_001

description: Library name: ENU54200B6G314_B11_neuro2052_S74_R1_001


title: ENU54200B6G311_B8_neuro2052_S74_R1_001

description: Library name: ENU54200B6G311_B8_neuro2052_S74_R1_001


title: ENU54200B6G312_B9_neuro2052_S74_R1_001

description: Library name: ENU54200B6G312_B9_neuro2052_S74_R1_001


title: ENU54200B6G310_B7_neuro2052_S74_R1_001

description: Library name: ENU54200B6G310_B7_neuro2052_S74_R1_001


title: ENU54188B6G36_A6_neuro2052_S73_R1_001

description: Library name: ENU54188B6G36_A6_neuro2052_S73_R1_001


title: ENU54188B6G35_A5_neuro2052_S73_R1_001

description: Library name: ENU54188B6G35_A5_neuro2052_S73_R1_001


title: ENU54188B6G33_A3_neuro2052_S73_R1_001

description: Library name: ENU54188B6G33_A3_neuro2052_S73_R1_001


title: ENU54188B6G34_A4_neuro2052_S73_R1_001

description: Library name: ENU54188B6G34_A4_neuro2052_S73_R1_001


title: ENU54188B6G32_A2_neuro2052_S73_R1_001

description: Library name: ENU54188B6G32_A2_neuro2052_S73_R1_001


title: ENU54188B6G31_A1_neuro2052_S73_R1_001

description: Library name: ENU54188B6G31_A1_neuro2052_S73_R1_001


title: ENU54188B6G314_A8_neuro2052_S73_R1_001

description: Library name: ENU54188B6G314_A8_neuro2052_S73_R1_001


title: ENU54188B6G317_A11_neuro2052_S73_R1_001

description: Library name: ENU54188B6G317_A11_neuro2052_S73_R1_001


title: ENU54188B6G319_A12_neuro2052_S73_R1_001

description: Library name: ENU54188B6G319_A12_neuro2052_S73_R1_001


title: ENU54188B6G315_A9_neuro2052_S73_R1_001

description: Library name: ENU54188B6G315_A9_neuro2052_S73_R1_001


title: ENU54188B6G316_A10_neuro2052_S73_R1_001

description: Library name: ENU54188B6G316_A10_neuro2052_S73_R1_001


title: ENU54187B6G39_F7_neuro2051_S70_R1_001

description: Library name: ENU54187B6G39_F7_neuro2051_S70_R1_001


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