description: Library name: ENU57344B6G38_A8_neuro2183_S89_R1_001_2367707189


status: Public on Apr 11 2026

type: SRA

city: South San Francisco

first: Mitchell

last: Rezzonico

state: CA

address: 1 DNA Way

country: USA

institute: Genentech

description: Library name: ENU57325B6G32_B2_neuro2173_S10_R1_001_2367707305

library_source: transcriptomic

data_processing:
RNA-seq reads were aligned to the mouse reference genome GRCm38 using GSNAP 74. Only uniquely mapping reads were carried forward to downstream analysis. Sample quality was further assessed on a per-plate basis based on total read counts, percentage of reads mapping to the reference genome, and percentage of reads mapping to exons. Reads predominantly mapped to transcript 3’ ends, as expected from the library preparation method.
Assembly: GRCm38

molecule: total RNA

organism: Mus musculus

source_name: Whole brain

growth_protocol: Founder C57BL/6NCrl males were treated with 3 x 100 mg/kg ENU (Sigma) and mutations bred to homozygosity in three generations. Generation 3 mice were collected usually between 8-12 weeks old, and an ear notch for DNA and brain samples were collected. Typically, 12 siblings from each pedigree were screened.

extract_protocol:
To homogenize the tissue, RNAlater was removed, and a 5 mm stainless steel bead (Qiagen) and 1.6 mL of Qiazol (Qiagen) was added. The tissue was homogenized using a Tissue Lyser II (Qiagen) for at least 30 minutes until a homogenous solution was confirmed. 400 µL of the homogenate solution was transferred into a new tube and allowed to sit for 5 minutes before adding 100 µL of chloroform (Sigma). The homogenate-chloroform mixture was shaken vigorously, allowed to stand for 2 minutes before centrifugation for 5 minutes at 4°C and 1000 x g. The top clear layer was removed and stored at -80 °C. For RNA extraction, 120 µL was transferred into a QIAgen S-block and processed on the QIAcube using the RNeasy 96 QIAcube HT Kit.
cDNA fragment libraries were constructed using the SMART-Seq® v4 3’ DE Kit (Takara Bio) following manufacturer’s recommendations, with minor modifications. Total RNA samples were processed in batches of 1152, distributed across twelve 96-well plates. For each sample, 4 ng total RNA was used as input for cDNA synthesis, then amplified for 8 cycles, with each sample in a row receiving a unique in-line index. Amplified cDNAs within a row were then combined into a 12-plex pool, cleaned up with Ampure XP beads (Beckman Coulter) and quantified using the Qubit HS DNA assay kit (Thermo Fisher Scientific). For each of the 96 cDNA pools generated from 12 plates, 400 pg were carried into Nextera XT library preparations (Illumina Inc.), each assigned with one of the 96 unique combinations of Forward and Reverse HT Index Primers included in the SMART-Seq® v4 3’ DE kit. After Ampure XP clean up and Qubit quantification, the 96 pools were combined in an equimolar manner to generate a 1152-plex Illumina library.  This library was concentrated with Ampure XP beads, quantified with Qubit and assessed on the TapeStation with the High Sensitivity D1000 assay (Agilent Technologies).

treatment_protocol: Mice were euthanized by cervical dislocation, then whole brains were collected. Each cerebellum was removed and the remaining brain matter was transferred to RNAlater and stored at 4C for up to 5 days.

tag: tissue

value: Whole brain

tag: partofsubset

value: FALSE


description: Library name: ENU57325B6G316_B12_neuro2173_S10_R1_001_2367707246


description: Library name: ENU57325B6G31_B1_neuro2173_S10_R1_001_2367707760


description: Library name: ENU57325B6G314_B10_neuro2173_S10_R1_001_2367707292


description: Library name: ENU57325B6G315_B11_neuro2173_S10_R1_001_2367707646


description: Library name: ENU57325B6G313_B9_neuro2173_S10_R1_001_2367707187


description: Library name: ENU57325B6G311_B7_neuro2173_S10_R1_001_2367706999


description: Library name: ENU57325B6G312_B8_neuro2173_S10_R1_001_2367706787


description: Library name: ENU57324B6G39_E1_neuro2183_S93_R1_001_2367706963


description: Library name: ENU57324B6G319_E11_neuro2183_S93_R1_001_2367707370


description: Library name: ENU57324B6G31_E7_neuro2183_S93_R1_001_2367706855


description: Library name: ENU57324B6G318_E10_neuro2183_S93_R1_001_2367707137


description: Library name: ENU57324B6G317_E9_neuro2183_S93_R1_001_2367707088


description: Library name: ENU57324B6G315_E6_neuro2183_S93_R1_001_2367707084


description: Library name: ENU57324B6G314_E5_neuro2183_S93_R1_001_2367706948


description: Library name: ENU57324B6G311_E3_neuro2183_S93_R1_001_2367707089


description: Library name: ENU57324B6G316_E8_neuro2183_S93_R1_001_2367707309


description: Library name: ENU57324B6G313_E4_neuro2183_S93_R1_001_2367707514


description: Library name: ENU57319B6G38_C6_neuro2173_S11_R1_001_2367707068


description: Library name: ENU57324B6G320_E12_neuro2183_S93_R1_001_2367707746


description: Library name: ENU57319B6G36_C4_neuro2173_S11_R1_001_2367707262


description: Library name: ENU57319B6G37_C5_neuro2173_S11_R1_001_2367707721


description: Library name: ENU57319B6G322_C12_neuro2173_S11_R1_001_2367707060


description: Library name: ENU57324B6G310_E2_neuro2183_S93_R1_001_2367707342


description: Library name: ENU57319B6G31_C1_neuro2173_S11_R1_001_2367707173


description: Library name: ENU57319B6G320_C10_neuro2173_S11_R1_001_2367707253


description: Library name: ENU57319B6G318_C8_neuro2173_S11_R1_001_2367707284


description: Library name: ENU57319B6G32_C2_neuro2173_S11_R1_001_2367707698


description: Library name: ENU57319B6G319_C9_neuro2173_S11_R1_001_2367707287


description: Library name: ENU57319B6G33_C3_neuro2173_S11_R1_001_2367707802


description: Library name: ENU57313B6G37_B5_neuro2176_S34_R1_001_2367707344


description: Library name: ENU57319B6G317_C7_neuro2173_S11_R1_001_2367707423


description: Library name: ENU57313B6G38_B6_neuro2176_S34_R1_001_2367707740


description: Library name: ENU57319B6G321_C11_neuro2173_S11_R1_001_2367707581


description: Library name: ENU57313B6G35_B3_neuro2176_S34_R1_001_2367706786


description: Library name: ENU57313B6G36_B4_neuro2176_S34_R1_001_2367706760


description: Library name: ENU57313B6G31_B1_neuro2176_S34_R1_001_2367707434


description: Library name: ENU57313B6G32_B2_neuro2176_S34_R1_001_2367707649


description: Library name: ENU57313B6G315_B9_neuro2176_S34_R1_001_2367707788


description: Library name: ENU57313B6G313_B7_neuro2176_S34_R1_001_2367707404


description: Library name: ENU57313B6G316_B10_neuro2176_S34_R1_001_2367706986


description: Library name: ENU57313B6G314_B8_neuro2176_S34_R1_001_2367707507


description: Library name: ENU57311B6G320_H11_neuro2174_S24_R1_001_2367707487


description: Library name: ENU57313B6G317_B11_neuro2176_S34_R1_001_2367707742


description: Library name: ENU57311B6G319_H7_neuro2174_S24_R1_001_2367707414


description: Library name: ENU57311B6G321_H12_neuro2174_S24_R1_001_2367707723


description: Library name: ENU57311B6G318_H6_neuro2174_S24_R1_001_2367707248


description: Library name: ENU57313B6G318_B12_neuro2176_S34_R1_001_2367707664


description: Library name: ENU57311B6G314_H5_neuro2174_S24_R1_001_2367707462


description: Library name: ENU57311B6G315_H8_neuro2174_S24_R1_001_2367706946


description: Library name: ENU57311B6G313_H4_neuro2174_S24_R1_001_2367706803


description: Library name: ENU57311B6G311_H2_neuro2174_S24_R1_001_2367707701


description: Library name: ENU57311B6G312_H3_neuro2174_S24_R1_001_2367706890


description: Library name: ENU57311B6G317_H10_neuro2174_S24_R1_001_2367706771


description: Library name: ENU57306B6G322_E12_neuro2176_S37_R1_001_2367707724


description: Library name: ENU57311B6G310_H1_neuro2174_S24_R1_001_2367706769


description: Library name: ENU57306B6G36_E1_neuro2176_S37_R1_001_2367707481


description: Library name: ENU57306B6G320_E10_neuro2176_S37_R1_001_2367707324


description: Library name: ENU57311B6G316_H9_neuro2174_S24_R1_001_2367707671


description: Library name: ENU57306B6G317_E8_neuro2176_S37_R1_001_2367707633


description: Library name: ENU57306B6G316_E7_neuro2176_S37_R1_001_2367707231


description: Library name: ENU57306B6G313_E3_neuro2176_S37_R1_001_2367707019


description: Library name: ENU57306B6G314_E4_neuro2176_S37_R1_001_2367707263


description: Library name: ENU57306B6G318_E5_neuro2176_S37_R1_001_2367707115


description: Library name: ENU57306B6G321_E11_neuro2176_S37_R1_001_2367707473


description: Library name: ENU57306B6G315_E6_neuro2176_S37_R1_001_2367706900


description: Library name: ENU57306B6G319_E9_neuro2176_S37_R1_001_2367706951


description: Library name: ENU57305B6G36_C6_neuro2181_S75_R1_001_2367707454


description: Library name: ENU57305B6G32_C2_neuro2181_S75_R1_001_2367707289


description: Library name: ENU57305B6G34_C4_neuro2181_S75_R1_001_2367707081


description: Library name: ENU57306B6G312_E2_neuro2176_S37_R1_001_2367707110


description: Library name: ENU57305B6G320_C7_neuro2181_S75_R1_001_2367706860


description: Library name: ENU57305B6G31_C1_neuro2181_S75_R1_001_2367707493


description: Library name: ENU57305B6G317_C10_neuro2181_S75_R1_001_2367707657


description: Library name: ENU57305B6G315_C8_neuro2181_S75_R1_001_2367707687


description: Library name: ENU57305B6G319_C12_neuro2181_S75_R1_001_2367707176


description: Library name: ENU57300B6G39_H7_neuro2176_S40_R1_001_2367707099


description: Library name: ENU57300B6G38_H6_neuro2176_S40_R1_001_2367707075


description: Library name: ENU57305B6G35_C5_neuro2181_S75_R1_001_2367707787


description: Library name: ENU57305B6G33_C3_neuro2181_S75_R1_001_2367707214


description: Library name: ENU57300B6G36_H3_neuro2176_S40_R1_001_2367706939


description: Library name: ENU57300B6G31_H1_neuro2176_S40_R1_001_2367707546


description: Library name: ENU57300B6G35_H2_neuro2176_S40_R1_001_2367706782


description: Library name: ENU57305B6G316_C9_neuro2181_S75_R1_001_2367707006


description: Library name: ENU57305B6G318_C11_neuro2181_S75_R1_001_2367706859


description: Library name: ENU57300B6G314_H5_neuro2176_S40_R1_001_2367707197


description: Library name: ENU57300B6G317_H12_neuro2176_S40_R1_001_2367707348


description: Library name: ENU57300B6G313_H4_neuro2176_S40_R1_001_2367707226


description: Library name: ENU57300B6G311_H9_neuro2176_S40_R1_001_2367707781


description: Library name: ENU57300B6G310_H8_neuro2176_S40_R1_001_2367707689


description: Library name: ENU57300B6G315_H10_neuro2176_S40_R1_001_2367707511


description: Library name: ENU57300B6G316_H11_neuro2176_S40_R1_001_2367707319


description: Library name: ENU57299B6G36_G3_neuro2177_S47_R1_001_2367707416


description: Library name: ENU57299B6G39_G6_neuro2177_S47_R1_001_2367706710


description: Library name: ENU57299B6G38_G5_neuro2177_S47_R1_001_2367706852


description: Library name: ENU57299B6G314_G11_neuro2177_S47_R1_001_2367706805


description: Library name: ENU57299B6G35_G2_neuro2177_S47_R1_001_2367707440


description: Library name: ENU57299B6G313_G10_neuro2177_S47_R1_001_2367707206


description: Library name: ENU57299B6G37_G4_neuro2177_S47_R1_001_2367707823


description: Library name: ENU57299B6G34_G1_neuro2177_S47_R1_001_2367707273


description: Library name: ENU57299B6G311_G8_neuro2177_S47_R1_001_2367706765


description: Library name: ENU57292B6G39_B9_neuro2182_S82_R1_001_2367706748


description: Library name: ENU57299B6G312_G9_neuro2177_S47_R1_001_2367706722


description: Library name: ENU57292B6G38_B8_neuro2182_S82_R1_001_2367706716


description: Library name: ENU57299B6G310_G7_neuro2177_S47_R1_001_2367707295


description: Library name: ENU57292B6G37_B7_neuro2182_S82_R1_001_2367707308


description: Library name: ENU57299B6G323_G12_neuro2177_S47_R1_001_2367707676


description: Library name: ENU57292B6G35_B5_neuro2182_S82_R1_001_2367707750


description: Library name: ENU57292B6G31_B1_neuro2182_S82_R1_001_2367707794


description: Library name: ENU57292B6G32_B2_neuro2182_S82_R1_001_2367707374


description: Library name: ENU57292B6G36_B6_neuro2182_S82_R1_001_2367706891


description: Library name: ENU57292B6G33_B3_neuro2182_S82_R1_001_2367706812


description: Library name: ENU57292B6G34_B4_neuro2182_S82_R1_001_2367707437


description: Library name: ENU57291B6G36_G6_neuro2173_S15_R1_001_2367707548


description: Library name: ENU57292B6G311_B11_neuro2182_S82_R1_001_2367707159


description: Library name: ENU57291B6G35_G5_neuro2173_S15_R1_001_2367706800


description: Library name: ENU57292B6G310_B10_neuro2182_S82_R1_001_2367707385


description: Library name: ENU57292B6G315_B12_neuro2182_S82_R1_001_2367707524


description: Library name: ENU57291B6G31_G1_neuro2173_S15_R1_001_2367707359


description: Library name: ENU57291B6G32_G2_neuro2173_S15_R1_001_2367707069


description: Library name: ENU57290B6G35_D5_neuro2172_S4_R1_001_2367707055


description: Library name: ENU57291B6G33_G3_neuro2173_S15_R1_001_2367707851


description: Library name: ENU57290B6G316_D12_neuro2172_S4_R1_001_2367707696


description: Library name: ENU57290B6G31_D1_neuro2172_S4_R1_001_2367707775


description: Library name: ENU57290B6G32_D2_neuro2172_S4_R1_001_2367706702


description: Library name: ENU57290B6G33_D3_neuro2172_S4_R1_001_2367707204


description: Library name: ENU57290B6G34_D4_neuro2172_S4_R1_001_2367707066


description: Library name: ENU57290B6G315_D11_neuro2172_S4_R1_001_2367707626


description: Library name: ENU57291B6G34_G4_neuro2173_S15_R1_001_2367706882


description: Library name: ENU57290B6G313_D9_neuro2172_S4_R1_001_2367706883


description: Library name: ENU57289B6G327_F11_neuro2176_S38_R1_001_2367707002


description: Library name: ENU57290B6G310_D6_neuro2172_S4_R1_001_2367706906


description: Library name: ENU57290B6G311_D7_neuro2172_S4_R1_001_2367707564


description: Library name: ENU57290B6G314_D10_neuro2172_S4_R1_001_2367706815


description: Library name: ENU57289B6G39_F1_neuro2176_S38_R1_001_2367707216


description: Library name: ENU57290B6G312_D8_neuro2172_S4_R1_001_2367706990


description: Library name: ENU57289B6G323_F10_neuro2176_S38_R1_001_2367707738


description: Library name: ENU57289B6G320_F7_neuro2176_S38_R1_001_2367707241


description: Library name: ENU57289B6G325_F12_neuro2176_S38_R1_001_2367707277


description: Library name: ENU57289B6G322_F9_neuro2176_S38_R1_001_2367707320


description: Library name: ENU57289B6G319_F6_neuro2176_S38_R1_001_2367707086


description: Library name: ENU57289B6G317_F4_neuro2176_S38_R1_001_2367707621


description: Library name: ENU57289B6G316_F3_neuro2176_S38_R1_001_2367707779


description: Library name: ENU57288B6G37_B4_neuro2178_S50_R1_001_2367706764


description: Library name: ENU57289B6G318_F5_neuro2176_S38_R1_001_2367707480


description: Library name: ENU57288B6G38_B5_neuro2178_S50_R1_001_2367707080


description: Library name: ENU57288B6G39_B6_neuro2178_S50_R1_001_2367707620


description: Library name: ENU57288B6G35_B3_neuro2178_S50_R1_001_2367707420


description: Library name: ENU57289B6G321_F8_neuro2176_S38_R1_001_2367707041


description: Library name: ENU57289B6G311_F2_neuro2176_S38_R1_001_2367706966


description: Library name: ENU57288B6G33_B1_neuro2178_S50_R1_001_2367707030


description: Library name: ENU57288B6G325_B12_neuro2178_S50_R1_001_2367707395


description: Library name: ENU57288B6G34_B2_neuro2178_S50_R1_001_2367707094


description: Library name: ENU57287B6G39_E3_neuro2175_S29_R1_001_2367707234


description: Library name: ENU57288B6G321_B9_neuro2178_S50_R1_001_2367707360


description: Library name: ENU57288B6G320_B8_neuro2178_S50_R1_001_2367707009


description: Library name: ENU57288B6G319_B7_neuro2178_S50_R1_001_2367707790


description: Library name: ENU57288B6G322_B10_neuro2178_S50_R1_001_2367707402


description: Library name: ENU57287B6G311_E5_neuro2175_S29_R1_001_2367707384


description: Library name: ENU57288B6G324_B11_neuro2178_S50_R1_001_2367706875


description: Library name: ENU57287B6G37_E1_neuro2175_S29_R1_001_2367707748


description: Library name: ENU57287B6G310_E4_neuro2175_S29_R1_001_2367707527


description: Library name: ENU57287B6G312_E6_neuro2175_S29_R1_001_2367707103


description: Library name: ENU57287B6G38_E2_neuro2175_S29_R1_001_2367707192


description: Library name: ENU57286B6G38_G7_neuro2182_S87_R1_001_2367707430


description: Library name: ENU57286B6G315_G11_neuro2182_S87_R1_001_2367706761


description: Library name: ENU57286B6G39_G8_neuro2182_S87_R1_001_2367707451


description: Library name: ENU57275B6G39_A2_neuro2178_S49_R1_001_2367707073


description: Library name: ENU57286B6G311_G10_neuro2182_S87_R1_001_2367707097


description: Library name: ENU57275B6G38_A1_neuro2178_S49_R1_001_2367707590


description: Library name: ENU57275B6G326_A11_neuro2178_S49_R1_001_2367707648


description: Library name: ENU57275B6G32_A4_neuro2178_S49_R1_001_2367706779


description: Library name: ENU57275B6G33_A5_neuro2178_S49_R1_001_2367707024


description: Library name: ENU57275B6G327_A12_neuro2178_S49_R1_001_2367707494


description: Library name: ENU57286B6G310_G9_neuro2182_S87_R1_001_2367707217


description: Library name: ENU57286B6G316_G12_neuro2182_S87_R1_001_2367707561


description: Library name: ENU57275B6G34_A6_neuro2178_S49_R1_001_2367707203


description: Library name: ENU57275B6G324_A9_neuro2178_S49_R1_001_2367707542


description: Library name: ENU57275B6G321_A8_neuro2178_S49_R1_001_2367707130


description: Library name: ENU57271B6G38_D3_neuro2182_S84_R1_001_2367706791


description: Library name: ENU57271B6G39_D4_neuro2182_S84_R1_001_2367707102


description: Library name: ENU57275B6G320_A7_neuro2178_S49_R1_001_2367707715


description: Library name: ENU57275B6G325_A10_neuro2178_S49_R1_001_2367707593


description: Library name: ENU57271B6G37_D2_neuro2182_S84_R1_001_2367707604


description: Library name: ENU57271B6G36_D1_neuro2182_S84_R1_001_2367706796


description: Library name: ENU57271B6G318_D12_neuro2182_S84_R1_001_2367706820


description: Library name: ENU57275B6G31_A3_neuro2178_S49_R1_001_2367706742


description: Library name: ENU57271B6G315_D9_neuro2182_S84_R1_001_2367707601


description: Library name: ENU57271B6G316_D10_neuro2182_S84_R1_001_2367707681


description: Library name: ENU56414B6G316_F11_neuro2193_S78_R1_001_2367705627


description: Library name: ENU57271B6G313_D7_neuro2182_S84_R1_001_2367707371


description: Library name: ENU57271B6G314_D8_neuro2182_S84_R1_001_2367706970


description: Library name: ENU57271B6G310_D5_neuro2182_S84_R1_001_2367706944


description: Library name: ENU57270B6G37_G7_neuro2172_S7_R1_001_2367706950


description: Library name: ENU57270B6G35_G5_neuro2172_S7_R1_001_2367707463


description: Library name: ENU57270B6G36_G6_neuro2172_S7_R1_001_2367707332


description: Library name: ENU57270B6G38_G4_neuro2172_S7_R1_001_2367707252


description: Library name: ENU57271B6G311_D6_neuro2182_S84_R1_001_2367706743


description: Library name: ENU57270B6G32_G2_neuro2172_S7_R1_001_2367707265


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description: Library name: ENU57271B6G317_D11_neuro2182_S84_R1_001_2367707811


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value: Slc44a1 C487X


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