title: ENU54194B6G36_A6_neuro2063_S65_R1_001

description: Library name: ENU54194B6G36_A6_neuro2063_S65_R1_001


title: ENU54194B6G34_A4_neuro2063_S65_R1_001

status: Public on Apr 11 2026

type: SRA

city: South San Francisco

first: Mitchell

last: Rezzonico

state: CA

address: 1 DNA Way

country: USA

institute: Genentech

description: Library name: ENU54194B6G34_A4_neuro2063_S65_R1_001

library_source: transcriptomic

data_processing:
RNA-seq reads were aligned to the mouse reference genome GRCm38 using GSNAP 74. Only uniquely mapping reads were carried forward to downstream analysis. Sample quality was further assessed on a per-plate basis based on total read counts, percentage of reads mapping to the reference genome, and percentage of reads mapping to exons. Reads predominantly mapped to transcript 3’ ends, as expected from the library preparation method.
Assembly: GRCm38

molecule: total RNA

organism: Mus musculus

source_name: Whole brain

growth_protocol: Founder C57BL/6NCrl males were treated with 3 x 100 mg/kg ENU (Sigma) and mutations bred to homozygosity in three generations. Generation 3 mice were collected usually between 8-12 weeks old, and an ear notch for DNA and brain samples were collected. Typically, 12 siblings from each pedigree were screened.

extract_protocol:
To homogenize the tissue, RNAlater was removed, and a 5 mm stainless steel bead (Qiagen) and 1.6 mL of Qiazol (Qiagen) was added. The tissue was homogenized using a Tissue Lyser II (Qiagen) for at least 30 minutes until a homogenous solution was confirmed. 400 µL of the homogenate solution was transferred into a new tube and allowed to sit for 5 minutes before adding 100 µL of chloroform (Sigma). The homogenate-chloroform mixture was shaken vigorously, allowed to stand for 2 minutes before centrifugation for 5 minutes at 4°C and 1000 x g. The top clear layer was removed and stored at -80 °C. For RNA extraction, 120 µL was transferred into a QIAgen S-block and processed on the QIAcube using the RNeasy 96 QIAcube HT Kit.
cDNA fragment libraries were constructed using the SMART-Seq® v4 3’ DE Kit (Takara Bio) following manufacturer’s recommendations, with minor modifications. Total RNA samples were processed in batches of 1152, distributed across twelve 96-well plates. For each sample, 4 ng total RNA was used as input for cDNA synthesis, then amplified for 8 cycles, with each sample in a row receiving a unique in-line index. Amplified cDNAs within a row were then combined into a 12-plex pool, cleaned up with Ampure XP beads (Beckman Coulter) and quantified using the Qubit HS DNA assay kit (Thermo Fisher Scientific). For each of the 96 cDNA pools generated from 12 plates, 400 pg were carried into Nextera XT library preparations (Illumina Inc.), each assigned with one of the 96 unique combinations of Forward and Reverse HT Index Primers included in the SMART-Seq® v4 3’ DE kit. After Ampure XP clean up and Qubit quantification, the 96 pools were combined in an equimolar manner to generate a 1152-plex Illumina library.  This library was concentrated with Ampure XP beads, quantified with Qubit and assessed on the TapeStation with the High Sensitivity D1000 assay (Agilent Technologies).

treatment_protocol: Mice were euthanized by cervical dislocation, then whole brains were collected. Each cerebellum was removed and the remaining brain matter was transferred to RNAlater and stored at 4C for up to 5 days.

tag: tissue

value: Whole brain

tag: partofsubset

value: FALSE


title: ENU54194B6G315_A11_neuro2063_S65_R1_001

description: Library name: ENU54194B6G315_A11_neuro2063_S65_R1_001


title: ENU54194B6G33_A3_neuro2063_S65_R1_001

description: Library name: ENU54194B6G33_A3_neuro2063_S65_R1_001


title: ENU54194B6G31_A1_neuro2063_S65_R1_001

description: Library name: ENU54194B6G31_A1_neuro2063_S65_R1_001


title: ENU54194B6G32_A2_neuro2063_S65_R1_001

description: Library name: ENU54194B6G32_A2_neuro2063_S65_R1_001


title: ENU54194B6G314_A10_neuro2063_S65_R1_001

description: Library name: ENU54194B6G314_A10_neuro2063_S65_R1_001


title: ENU54194B6G316_A12_neuro2063_S65_R1_001

description: Library name: ENU54194B6G316_A12_neuro2063_S65_R1_001


title: ENU54194B6G313_A9_neuro2063_S65_R1_001

description: Library name: ENU54194B6G313_A9_neuro2063_S65_R1_001


title: ENU54191B6G322_C12_neuro2060_S43_R1_001

description: Library name: ENU54191B6G322_C12_neuro2060_S43_R1_001


title: ENU54194B6G312_A8_neuro2063_S65_R1_001

description: Library name: ENU54194B6G312_A8_neuro2063_S65_R1_001


title: ENU54191B6G319_C9_neuro2060_S43_R1_001

description: Library name: ENU54191B6G319_C9_neuro2060_S43_R1_001


title: ENU54191B6G321_C11_neuro2060_S43_R1_001

description: Library name: ENU54191B6G321_C11_neuro2060_S43_R1_001


title: ENU54194B6G35_A5_neuro2063_S65_R1_001

description: Library name: ENU54194B6G35_A5_neuro2063_S65_R1_001


title: ENU54191B6G318_C8_neuro2060_S43_R1_001

description: Library name: ENU54191B6G318_C8_neuro2060_S43_R1_001


title: ENU54191B6G320_C10_neuro2060_S43_R1_001

description: Library name: ENU54191B6G320_C10_neuro2060_S43_R1_001


title: ENU54191B6G317_C7_neuro2060_S43_R1_001

description: Library name: ENU54191B6G317_C7_neuro2060_S43_R1_001


title: ENU54191B6G315_C6_neuro2060_S43_R1_001

description: Library name: ENU54191B6G315_C6_neuro2060_S43_R1_001


title: ENU54191B6G316_C4_neuro2060_S43_R1_001

description: Library name: ENU54191B6G316_C4_neuro2060_S43_R1_001


title: ENU54191B6G314_C5_neuro2060_S43_R1_001

description: Library name: ENU54191B6G314_C5_neuro2060_S43_R1_001


title: ENU54191B6G312_C2_neuro2060_S43_R1_001

description: Library name: ENU54191B6G312_C2_neuro2060_S43_R1_001


title: ENU54191B6G313_C3_neuro2060_S43_R1_001

description: Library name: ENU54191B6G313_C3_neuro2060_S43_R1_001


title: ENU54191B6G311_C1_neuro2060_S43_R1_001

description: Library name: ENU54191B6G311_C1_neuro2060_S43_R1_001


title: ENU54189B6G36_B8_neuro2060_S42_R1_001

description: Library name: ENU54189B6G36_B8_neuro2060_S42_R1_001


title: ENU54189B6G35_B7_neuro2060_S42_R1_001

description: Library name: ENU54189B6G35_B7_neuro2060_S42_R1_001


title: ENU54189B6G39_B5_neuro2060_S42_R1_001

description: Library name: ENU54189B6G39_B5_neuro2060_S42_R1_001


title: ENU54189B6G34_B4_neuro2060_S42_R1_001

description: Library name: ENU54189B6G34_B4_neuro2060_S42_R1_001


title: ENU54189B6G37_B9_neuro2060_S42_R1_001

description: Library name: ENU54189B6G37_B9_neuro2060_S42_R1_001


title: ENU54189B6G33_B3_neuro2060_S42_R1_001

description: Library name: ENU54189B6G33_B3_neuro2060_S42_R1_001


title: ENU54189B6G31_B1_neuro2060_S42_R1_001

description: Library name: ENU54189B6G31_B1_neuro2060_S42_R1_001


title: ENU54189B6G32_B2_neuro2060_S42_R1_001

description: Library name: ENU54189B6G32_B2_neuro2060_S42_R1_001


title: ENU54189B6G313_B10_neuro2060_S42_R1_001

description: Library name: ENU54189B6G313_B10_neuro2060_S42_R1_001


title: ENU54189B6G314_B11_neuro2060_S42_R1_001

description: Library name: ENU54189B6G314_B11_neuro2060_S42_R1_001


title: ENU54189B6G310_B6_neuro2060_S42_R1_001

description: Library name: ENU54189B6G310_B6_neuro2060_S42_R1_001


title: ENU54184B6G36_D6_neuro2055_S4_R1_001

description: Library name: ENU54184B6G36_D6_neuro2055_S4_R1_001


title: ENU54189B6G315_B12_neuro2060_S42_R1_001

description: Library name: ENU54189B6G315_B12_neuro2060_S42_R1_001


title: ENU54184B6G33_D3_neuro2055_S4_R1_001

description: Library name: ENU54184B6G33_D3_neuro2055_S4_R1_001


title: ENU54184B6G37_D5_neuro2055_S4_R1_001

description: Library name: ENU54184B6G37_D5_neuro2055_S4_R1_001


title: ENU54184B6G34_D4_neuro2055_S4_R1_001

description: Library name: ENU54184B6G34_D4_neuro2055_S4_R1_001


title: ENU54184B6G32_D2_neuro2055_S4_R1_001

description: Library name: ENU54184B6G32_D2_neuro2055_S4_R1_001


title: ENU54184B6G31_D1_neuro2055_S4_R1_001

description: Library name: ENU54184B6G31_D1_neuro2055_S4_R1_001


title: ENU54184B6G314_D11_neuro2055_S4_R1_001

description: Library name: ENU54184B6G314_D11_neuro2055_S4_R1_001


title: ENU54184B6G313_D10_neuro2055_S4_R1_001

description: Library name: ENU54184B6G313_D10_neuro2055_S4_R1_001


title: ENU54184B6G311_D8_neuro2055_S4_R1_001

description: Library name: ENU54184B6G311_D8_neuro2055_S4_R1_001


title: ENU54184B6G312_D9_neuro2055_S4_R1_001

description: Library name: ENU54184B6G312_D9_neuro2055_S4_R1_001


title: ENU54184B6G310_D7_neuro2055_S4_R1_001

description: Library name: ENU54184B6G310_D7_neuro2055_S4_R1_001


title: ENU54182B6G39_C8_neuro2055_S3_R1_001

description: Library name: ENU54182B6G39_C8_neuro2055_S3_R1_001


title: ENU54182B6G38_C7_neuro2055_S3_R1_001

description: Library name: ENU54182B6G38_C7_neuro2055_S3_R1_001


title: ENU54182B6G36_C4_neuro2055_S3_R1_001

description: Library name: ENU54182B6G36_C4_neuro2055_S3_R1_001


title: ENU54182B6G37_C5_neuro2055_S3_R1_001

description: Library name: ENU54182B6G37_C5_neuro2055_S3_R1_001


title: ENU54182B6G35_C3_neuro2055_S3_R1_001

description: Library name: ENU54182B6G35_C3_neuro2055_S3_R1_001


title: ENU54182B6G32_C2_neuro2055_S3_R1_001

description: Library name: ENU54182B6G32_C2_neuro2055_S3_R1_001


title: ENU54184B6G315_D12_neuro2055_S4_R1_001

description: Library name: ENU54184B6G315_D12_neuro2055_S4_R1_001


title: ENU54182B6G316_C10_neuro2055_S3_R1_001

description: Library name: ENU54182B6G316_C10_neuro2055_S3_R1_001


title: ENU54182B6G34_C6_neuro2055_S3_R1_001

description: Library name: ENU54182B6G34_C6_neuro2055_S3_R1_001


title: ENU54182B6G315_C12_neuro2055_S3_R1_001

description: Library name: ENU54182B6G315_C12_neuro2055_S3_R1_001


title: ENU54182B6G310_C9_neuro2055_S3_R1_001

description: Library name: ENU54182B6G310_C9_neuro2055_S3_R1_001


title: ENU54182B6G31_C1_neuro2055_S3_R1_001

description: Library name: ENU54182B6G31_C1_neuro2055_S3_R1_001


title: ENU54182B6G314_C11_neuro2055_S3_R1_001

description: Library name: ENU54182B6G314_C11_neuro2055_S3_R1_001


title: ENU54181B6G322_B10_neuro2055_S2_R1_001

description: Library name: ENU54181B6G322_B10_neuro2055_S2_R1_001


title: ENU54181B6G323_B12_neuro2055_S2_R1_001

description: Library name: ENU54181B6G323_B12_neuro2055_S2_R1_001


title: ENU54181B6G39_B3_neuro2055_S2_R1_001

description: Library name: ENU54181B6G39_B3_neuro2055_S2_R1_001


title: ENU54181B6G320_B8_neuro2055_S2_R1_001

description: Library name: ENU54181B6G320_B8_neuro2055_S2_R1_001


title: ENU54181B6G319_B7_neuro2055_S2_R1_001

description: Library name: ENU54181B6G319_B7_neuro2055_S2_R1_001


title: ENU54181B6G318_B6_neuro2055_S2_R1_001

description: Library name: ENU54181B6G318_B6_neuro2055_S2_R1_001


title: ENU54181B6G317_B5_neuro2055_S2_R1_001

description: Library name: ENU54181B6G317_B5_neuro2055_S2_R1_001


title: ENU54181B6G316_B11_neuro2055_S2_R1_001

description: Library name: ENU54181B6G316_B11_neuro2055_S2_R1_001


title: ENU54181B6G311_B2_neuro2055_S2_R1_001

description: Library name: ENU54181B6G311_B2_neuro2055_S2_R1_001


title: ENU54181B6G321_B9_neuro2055_S2_R1_001

description: Library name: ENU54181B6G321_B9_neuro2055_S2_R1_001


title: ENU54175B6G39_G9_neuro2056_S15_R1_001

description: Library name: ENU54175B6G39_G9_neuro2056_S15_R1_001


title: ENU54181B6G310_B1_neuro2055_S2_R1_001

description: Library name: ENU54181B6G310_B1_neuro2055_S2_R1_001


title: ENU54175B6G37_G4_neuro2056_S15_R1_001

description: Library name: ENU54175B6G37_G4_neuro2056_S15_R1_001


title: ENU54175B6G35_G6_neuro2056_S15_R1_001

description: Library name: ENU54175B6G35_G6_neuro2056_S15_R1_001


title: ENU54175B6G36_G7_neuro2056_S15_R1_001

description: Library name: ENU54175B6G36_G7_neuro2056_S15_R1_001


title: ENU54181B6G312_B4_neuro2055_S2_R1_001

description: Library name: ENU54181B6G312_B4_neuro2055_S2_R1_001


title: ENU54175B6G33_G3_neuro2056_S15_R1_001

description: Library name: ENU54175B6G33_G3_neuro2056_S15_R1_001


title: ENU54175B6G38_G8_neuro2056_S15_R1_001

description: Library name: ENU54175B6G38_G8_neuro2056_S15_R1_001


title: ENU54175B6G329_G11_neuro2056_S15_R1_001

description: Library name: ENU54175B6G329_G11_neuro2056_S15_R1_001


title: ENU54175B6G32_G2_neuro2056_S15_R1_001

description: Library name: ENU54175B6G32_G2_neuro2056_S15_R1_001


title: ENU54175B6G330_G12_neuro2056_S15_R1_001

description: Library name: ENU54175B6G330_G12_neuro2056_S15_R1_001


title: ENU54175B6G34_G5_neuro2056_S15_R1_001

description: Library name: ENU54175B6G34_G5_neuro2056_S15_R1_001


title: ENU54175B6G31_G1_neuro2056_S15_R1_001

description: Library name: ENU54175B6G31_G1_neuro2056_S15_R1_001


title: ENU54174B6G39_F6_neuro2056_S14_R1_001

description: Library name: ENU54174B6G39_F6_neuro2056_S14_R1_001


title: ENU54175B6G314_G10_neuro2056_S15_R1_001

description: Library name: ENU54175B6G314_G10_neuro2056_S15_R1_001


title: ENU54174B6G32_F2_neuro2056_S14_R1_001

description: Library name: ENU54174B6G32_F2_neuro2056_S14_R1_001


title: ENU54174B6G33_F3_neuro2056_S14_R1_001

description: Library name: ENU54174B6G33_F3_neuro2056_S14_R1_001


title: ENU54174B6G38_F5_neuro2056_S14_R1_001

description: Library name: ENU54174B6G38_F5_neuro2056_S14_R1_001


title: ENU54174B6G31_F1_neuro2056_S14_R1_001

description: Library name: ENU54174B6G31_F1_neuro2056_S14_R1_001


title: ENU54174B6G313_F10_neuro2056_S14_R1_001

description: Library name: ENU54174B6G313_F10_neuro2056_S14_R1_001


title: ENU54174B6G314_F11_neuro2056_S14_R1_001

description: Library name: ENU54174B6G314_F11_neuro2056_S14_R1_001


title: ENU54174B6G310_F7_neuro2056_S14_R1_001

description: Library name: ENU54174B6G310_F7_neuro2056_S14_R1_001


title: ENU54174B6G34_F4_neuro2056_S14_R1_001

description: Library name: ENU54174B6G34_F4_neuro2056_S14_R1_001


title: ENU54174B6G315_F12_neuro2056_S14_R1_001

description: Library name: ENU54174B6G315_F12_neuro2056_S14_R1_001


title: ENU54174B6G311_F8_neuro2056_S14_R1_001

description: Library name: ENU54174B6G311_F8_neuro2056_S14_R1_001


title: ENU54174B6G312_F9_neuro2056_S14_R1_001

description: Library name: ENU54174B6G312_F9_neuro2056_S14_R1_001


title: ENU54170B6G39_F6_neuro2065_S78_R1_001

description: Library name: ENU54170B6G39_F6_neuro2065_S78_R1_001


title: ENU54170B6G36_F12_neuro2065_S78_R1_001

description: Library name: ENU54170B6G36_F12_neuro2065_S78_R1_001


title: ENU54170B6G33_F3_neuro2065_S78_R1_001

description: Library name: ENU54170B6G33_F3_neuro2065_S78_R1_001


title: ENU54170B6G35_F5_neuro2065_S78_R1_001

description: Library name: ENU54170B6G35_F5_neuro2065_S78_R1_001


title: ENU54170B6G32_F2_neuro2065_S78_R1_001

description: Library name: ENU54170B6G32_F2_neuro2065_S78_R1_001


title: ENU54170B6G313_F10_neuro2065_S78_R1_001

description: Library name: ENU54170B6G313_F10_neuro2065_S78_R1_001


title: ENU54170B6G31_F1_neuro2065_S78_R1_001

description: Library name: ENU54170B6G31_F1_neuro2065_S78_R1_001


title: ENU54170B6G314_F11_neuro2065_S78_R1_001

description: Library name: ENU54170B6G314_F11_neuro2065_S78_R1_001


title: ENU54170B6G312_F9_neuro2065_S78_R1_001

description: Library name: ENU54170B6G312_F9_neuro2065_S78_R1_001


title: ENU54170B6G311_F8_neuro2065_S78_R1_001

description: Library name: ENU54170B6G311_F8_neuro2065_S78_R1_001


title: ENU54170B6G34_F4_neuro2065_S78_R1_001

description: Library name: ENU54170B6G34_F4_neuro2065_S78_R1_001


title: ENU54168B6G36_D6_neuro2065_S76_R1_001

description: Library name: ENU54168B6G36_D6_neuro2065_S76_R1_001


title: ENU54168B6G35_D5_neuro2065_S76_R1_001

description: Library name: ENU54168B6G35_D5_neuro2065_S76_R1_001


title: ENU54168B6G33_D3_neuro2065_S76_R1_001

description: Library name: ENU54168B6G33_D3_neuro2065_S76_R1_001


title: ENU54170B6G310_F7_neuro2065_S78_R1_001

description: Library name: ENU54170B6G310_F7_neuro2065_S78_R1_001


title: ENU54168B6G32_D2_neuro2065_S76_R1_001

description: Library name: ENU54168B6G32_D2_neuro2065_S76_R1_001


title: ENU54168B6G31_D1_neuro2065_S76_R1_001

description: Library name: ENU54168B6G31_D1_neuro2065_S76_R1_001


title: ENU54168B6G318_D11_neuro2065_S76_R1_001

description: Library name: ENU54168B6G318_D11_neuro2065_S76_R1_001


title: ENU54168B6G34_D4_neuro2065_S76_R1_001

description: Library name: ENU54168B6G34_D4_neuro2065_S76_R1_001


title: ENU54168B6G319_D12_neuro2065_S76_R1_001

description: Library name: ENU54168B6G319_D12_neuro2065_S76_R1_001


title: ENU54168B6G316_D9_neuro2065_S76_R1_001

description: Library name: ENU54168B6G316_D9_neuro2065_S76_R1_001


title: ENU54168B6G315_D8_neuro2065_S76_R1_001

description: Library name: ENU54168B6G315_D8_neuro2065_S76_R1_001


title: ENU54168B6G314_D7_neuro2065_S76_R1_001

description: Library name: ENU54168B6G314_D7_neuro2065_S76_R1_001


title: ENU54168B6G317_D10_neuro2065_S76_R1_001

description: Library name: ENU54168B6G317_D10_neuro2065_S76_R1_001


title: ENU54163B6G37_E1_neuro2065_S77_R1_001

description: Library name: ENU54163B6G37_E1_neuro2065_S77_R1_001


title: ENU54163B6G322_E12_neuro2065_S77_R1_001

description: Library name: ENU54163B6G322_E12_neuro2065_S77_R1_001


title: ENU54163B6G321_E11_neuro2065_S77_R1_001

description: Library name: ENU54163B6G321_E11_neuro2065_S77_R1_001


title: ENU54163B6G319_E9_neuro2065_S77_R1_001

description: Library name: ENU54163B6G319_E9_neuro2065_S77_R1_001


title: ENU54163B6G318_E8_neuro2065_S77_R1_001

description: Library name: ENU54163B6G318_E8_neuro2065_S77_R1_001


title: ENU54163B6G314_E6_neuro2065_S77_R1_001

description: Library name: ENU54163B6G314_E6_neuro2065_S77_R1_001


title: ENU54163B6G315_E7_neuro2065_S77_R1_001

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title: ENU53171B6G36_A6_neuro2006_S33_R1_001

description: Library name: ENU53171B6G36_A6_neuro2006_S33_R1_001


title: ENU53171B6G37_A7_neuro2006_S33_R1_001

description: Library name: ENU53171B6G37_A7_neuro2006_S33_R1_001


title: ENU53171B6G33_A3_neuro2006_S33_R1_001

description: Library name: ENU53171B6G33_A3_neuro2006_S33_R1_001


title: ENU53171B6G35_A5_neuro2006_S33_R1_001

description: Library name: ENU53171B6G35_A5_neuro2006_S33_R1_001


title: ENU53171B6G34_A4_neuro2006_S33_R1_001

description: Library name: ENU53171B6G34_A4_neuro2006_S33_R1_001


title: ENU53171B6G31_A1_neuro2006_S33_R1_001

description: Library name: ENU53171B6G31_A1_neuro2006_S33_R1_001


title: ENU53171B6G312_A12_neuro2006_S33_R1_001

description: Library name: ENU53171B6G312_A12_neuro2006_S33_R1_001


title: ENU53171B6G39_A9_neuro2006_S33_R1_001

description: Library name: ENU53171B6G39_A9_neuro2006_S33_R1_001


title: ENU53171B6G310_A10_neuro2006_S33_R1_001

description: Library name: ENU53171B6G310_A10_neuro2006_S33_R1_001


title: ENU53171B6G311_A11_neuro2006_S33_R1_001

description: Library name: ENU53171B6G311_A11_neuro2006_S33_R1_001


title: ENU53169B6G38_F7_neuro2009_S62_R1_001

description: Library name: ENU53169B6G38_F7_neuro2009_S62_R1_001


title: ENU53169B6G39_F8_neuro2009_S62_R1_001

description: Library name: ENU53169B6G39_F8_neuro2009_S62_R1_001


title: ENU53171B6G32_A2_neuro2006_S33_R1_001

description: Library name: ENU53171B6G32_A2_neuro2006_S33_R1_001