title: ENU54283B6G32_C2_neuro2085_S51_R1_001

description: Library name: ENU54283B6G32_C2_neuro2085_S51_R1_001


title: ENU54283B6G320_C11_neuro2085_S51_R1_001

status: Public on Apr 11 2026

type: SRA

city: South San Francisco

first: Mitchell

last: Rezzonico

state: CA

address: 1 DNA Way

country: USA

institute: Genentech

description: Library name: ENU54283B6G320_C11_neuro2085_S51_R1_001

library_source: transcriptomic

data_processing:
RNA-seq reads were aligned to the mouse reference genome GRCm38 using GSNAP 74. Only uniquely mapping reads were carried forward to downstream analysis. Sample quality was further assessed on a per-plate basis based on total read counts, percentage of reads mapping to the reference genome, and percentage of reads mapping to exons. Reads predominantly mapped to transcript 3’ ends, as expected from the library preparation method.
Assembly: GRCm38

molecule: total RNA

organism: Mus musculus

source_name: Whole brain

growth_protocol: Founder C57BL/6NCrl males were treated with 3 x 100 mg/kg ENU (Sigma) and mutations bred to homozygosity in three generations. Generation 3 mice were collected usually between 8-12 weeks old, and an ear notch for DNA and brain samples were collected. Typically, 12 siblings from each pedigree were screened.

extract_protocol:
To homogenize the tissue, RNAlater was removed, and a 5 mm stainless steel bead (Qiagen) and 1.6 mL of Qiazol (Qiagen) was added. The tissue was homogenized using a Tissue Lyser II (Qiagen) for at least 30 minutes until a homogenous solution was confirmed. 400 µL of the homogenate solution was transferred into a new tube and allowed to sit for 5 minutes before adding 100 µL of chloroform (Sigma). The homogenate-chloroform mixture was shaken vigorously, allowed to stand for 2 minutes before centrifugation for 5 minutes at 4°C and 1000 x g. The top clear layer was removed and stored at -80 °C. For RNA extraction, 120 µL was transferred into a QIAgen S-block and processed on the QIAcube using the RNeasy 96 QIAcube HT Kit.
cDNA fragment libraries were constructed using the SMART-Seq® v4 3’ DE Kit (Takara Bio) following manufacturer’s recommendations, with minor modifications. Total RNA samples were processed in batches of 1152, distributed across twelve 96-well plates. For each sample, 4 ng total RNA was used as input for cDNA synthesis, then amplified for 8 cycles, with each sample in a row receiving a unique in-line index. Amplified cDNAs within a row were then combined into a 12-plex pool, cleaned up with Ampure XP beads (Beckman Coulter) and quantified using the Qubit HS DNA assay kit (Thermo Fisher Scientific). For each of the 96 cDNA pools generated from 12 plates, 400 pg were carried into Nextera XT library preparations (Illumina Inc.), each assigned with one of the 96 unique combinations of Forward and Reverse HT Index Primers included in the SMART-Seq® v4 3’ DE kit. After Ampure XP clean up and Qubit quantification, the 96 pools were combined in an equimolar manner to generate a 1152-plex Illumina library.  This library was concentrated with Ampure XP beads, quantified with Qubit and assessed on the TapeStation with the High Sensitivity D1000 assay (Agilent Technologies).

treatment_protocol: Mice were euthanized by cervical dislocation, then whole brains were collected. Each cerebellum was removed and the remaining brain matter was transferred to RNAlater and stored at 4C for up to 5 days.

tag: tissue

value: Whole brain

tag: partofsubset

value: FALSE


title: ENU54283B6G320_C11_neuro2085_S43_R1_001

description: Library name: ENU54283B6G320_C11_neuro2085_S43_R1_001


title: ENU54283B6G321_C12_neuro2085_S43_R1_001

description: Library name: ENU54283B6G321_C12_neuro2085_S43_R1_001


title: ENU54283B6G31_C1_neuro2085_S43_R1_001

description: Library name: ENU54283B6G31_C1_neuro2085_S43_R1_001


title: ENU54283B6G313_C5_neuro2085_S43_R1_001

description: Library name: ENU54283B6G313_C5_neuro2085_S43_R1_001


title: ENU54283B6G314_C6_neuro2085_S43_R1_001

description: Library name: ENU54283B6G314_C6_neuro2085_S43_R1_001


title: ENU54283B6G312_C4_neuro2085_S43_R1_001

description: Library name: ENU54283B6G312_C4_neuro2085_S43_R1_001


title: ENU54283B6G314_C6_neuro2085_S51_R1_001

description: Library name: ENU54283B6G314_C6_neuro2085_S51_R1_001


title: ENU54283B6G311_C3_neuro2085_S43_R1_001

description: Library name: ENU54283B6G311_C3_neuro2085_S43_R1_001


title: ENU54283B6G313_C5_neuro2085_S51_R1_001

description: Library name: ENU54283B6G313_C5_neuro2085_S51_R1_001


title: ENU54279B6G37_E7_neuro2080_S5_R1_001

description: Library name: ENU54279B6G37_E7_neuro2080_S5_R1_001


title: ENU54283B6G311_C3_neuro2085_S51_R1_001

description: Library name: ENU54283B6G311_C3_neuro2085_S51_R1_001


title: ENU54279B6G38_E8_neuro2080_S5_R1_001

description: Library name: ENU54279B6G38_E8_neuro2080_S5_R1_001


title: ENU54283B6G312_C4_neuro2085_S51_R1_001

description: Library name: ENU54283B6G312_C4_neuro2085_S51_R1_001


title: ENU54279B6G39_E9_neuro2080_S5_R1_001

description: Library name: ENU54279B6G39_E9_neuro2080_S5_R1_001


title: ENU54279B6G36_E3_neuro2080_S5_R1_001

description: Library name: ENU54279B6G36_E3_neuro2080_S5_R1_001


title: ENU54283B6G31_C1_neuro2085_S51_R1_001

description: Library name: ENU54283B6G31_C1_neuro2085_S51_R1_001


title: ENU54279B6G33_E4_neuro2080_S5_R1_001

description: Library name: ENU54279B6G33_E4_neuro2080_S5_R1_001


title: ENU54279B6G34_E5_neuro2080_S5_R1_001

description: Library name: ENU54279B6G34_E5_neuro2080_S5_R1_001


title: ENU54279B6G32_E2_neuro2080_S5_R1_001

description: Library name: ENU54279B6G32_E2_neuro2080_S5_R1_001


title: ENU54279B6G31_E1_neuro2080_S5_R1_001

description: Library name: ENU54279B6G31_E1_neuro2080_S5_R1_001


title: ENU54279B6G35_E6_neuro2080_S5_R1_001

description: Library name: ENU54279B6G35_E6_neuro2080_S5_R1_001


title: ENU54279B6G312_E12_neuro2080_S5_R1_001

description: Library name: ENU54279B6G312_E12_neuro2080_S5_R1_001


title: ENU54279B6G311_E11_neuro2080_S5_R1_001

description: Library name: ENU54279B6G311_E11_neuro2080_S5_R1_001


title: ENU54275B6G31_F1_neuro2075_S78_R1_001

description: Library name: ENU54275B6G31_F1_neuro2075_S78_R1_001


title: ENU54275B6G322_F12_neuro2075_S78_R1_001

description: Library name: ENU54275B6G322_F12_neuro2075_S78_R1_001


title: ENU54275B6G320_F10_neuro2075_S78_R1_001

description: Library name: ENU54275B6G320_F10_neuro2075_S78_R1_001


title: ENU54275B6G32_F2_neuro2075_S78_R1_001

description: Library name: ENU54275B6G32_F2_neuro2075_S78_R1_001


title: ENU54275B6G319_F9_neuro2075_S78_R1_001

description: Library name: ENU54275B6G319_F9_neuro2075_S78_R1_001


title: ENU54275B6G317_F7_neuro2075_S78_R1_001

description: Library name: ENU54275B6G317_F7_neuro2075_S78_R1_001


title: ENU54275B6G34_F4_neuro2075_S78_R1_001

description: Library name: ENU54275B6G34_F4_neuro2075_S78_R1_001


title: ENU54275B6G318_F8_neuro2075_S78_R1_001

description: Library name: ENU54275B6G318_F8_neuro2075_S78_R1_001


title: ENU54275B6G321_F11_neuro2075_S78_R1_001

description: Library name: ENU54275B6G321_F11_neuro2075_S78_R1_001


title: ENU54272B6G38_B6_neuro2085_S50_R1_001

description: Library name: ENU54272B6G38_B6_neuro2085_S50_R1_001


title: ENU54279B6G310_E10_neuro2080_S5_R1_001

description: Library name: ENU54279B6G310_E10_neuro2080_S5_R1_001


title: ENU54275B6G35_F5_neuro2075_S78_R1_001

description: Library name: ENU54275B6G35_F5_neuro2075_S78_R1_001


title: ENU54272B6G38_B6_neuro2085_S42_R1_001

description: Library name: ENU54272B6G38_B6_neuro2085_S42_R1_001

value: TRUE

value: Tex2 K994E


title: ENU54272B6G34_B12_neuro2085_S42_R1_001

description: Library name: ENU54272B6G34_B12_neuro2085_S42_R1_001


title: ENU54275B6G36_F6_neuro2075_S78_R1_001

description: Library name: ENU54275B6G36_F6_neuro2075_S78_R1_001


title: ENU54272B6G39_B7_neuro2085_S50_R1_001

description: Library name: ENU54272B6G39_B7_neuro2085_S50_R1_001


title: ENU54272B6G35_B4_neuro2085_S50_R1_001

description: Library name: ENU54272B6G35_B4_neuro2085_S50_R1_001


title: ENU54272B6G34_B12_neuro2085_S50_R1_001

description: Library name: ENU54272B6G34_B12_neuro2085_S50_R1_001


title: ENU54275B6G33_F3_neuro2075_S78_R1_001

description: Library name: ENU54275B6G33_F3_neuro2075_S78_R1_001


title: ENU54272B6G37_B5_neuro2085_S50_R1_001

description: Library name: ENU54272B6G37_B5_neuro2085_S50_R1_001


title: ENU54272B6G35_B4_neuro2085_S42_R1_001

description: Library name: ENU54272B6G35_B4_neuro2085_S42_R1_001


description: Library name: ENU54274B6G315_B11_neuro2003rep_S90_R1_001


title: ENU54272B6G39_B7_neuro2085_S42_R1_001

description: Library name: ENU54272B6G39_B7_neuro2085_S42_R1_001

value: DAM

value: m/m


description: Library name: ENU54274B6G31_B10_neuro2003rep_S90_R1_001


title: ENU54272B6G37_B5_neuro2085_S42_R1_001

description: Library name: ENU54272B6G37_B5_neuro2085_S42_R1_001


title: ENU54272B6G33_B3_neuro2085_S42_R1_001

description: Library name: ENU54272B6G33_B3_neuro2085_S42_R1_001


title: ENU54272B6G33_B3_neuro2085_S50_R1_001

description: Library name: ENU54272B6G33_B3_neuro2085_S50_R1_001


title: ENU54272B6G32_B2_neuro2085_S50_R1_001

description: Library name: ENU54272B6G32_B2_neuro2085_S50_R1_001


title: ENU54272B6G32_B2_neuro2085_S42_R1_001

description: Library name: ENU54272B6G32_B2_neuro2085_S42_R1_001


title: ENU54272B6G313_B11_neuro2085_S42_R1_001

description: Library name: ENU54272B6G313_B11_neuro2085_S42_R1_001


title: ENU54272B6G31_B1_neuro2085_S42_R1_001

description: Library name: ENU54272B6G31_B1_neuro2085_S42_R1_001


title: ENU54272B6G312_B10_neuro2085_S42_R1_001

description: Library name: ENU54272B6G312_B10_neuro2085_S42_R1_001


title: ENU54269B6G38_F7_neuro2081_S14_R1_001

description: Library name: ENU54269B6G38_F7_neuro2081_S14_R1_001


title: ENU54272B6G31_B1_neuro2085_S50_R1_001

description: Library name: ENU54272B6G31_B1_neuro2085_S50_R1_001


title: ENU54272B6G313_B11_neuro2085_S50_R1_001

description: Library name: ENU54272B6G313_B11_neuro2085_S50_R1_001


title: ENU54269B6G39_F8_neuro2081_S14_R1_001

description: Library name: ENU54269B6G39_F8_neuro2081_S14_R1_001


title: ENU54272B6G311_B9_neuro2085_S50_R1_001

description: Library name: ENU54272B6G311_B9_neuro2085_S50_R1_001


title: ENU54269B6G36_F5_neuro2081_S14_R1_001

description: Library name: ENU54269B6G36_F5_neuro2081_S14_R1_001


title: ENU54272B6G311_B9_neuro2085_S42_R1_001

description: Library name: ENU54272B6G311_B9_neuro2085_S42_R1_001


title: ENU54269B6G35_F4_neuro2081_S14_R1_001

description: Library name: ENU54269B6G35_F4_neuro2081_S14_R1_001


title: ENU54269B6G34_F3_neuro2081_S14_R1_001

description: Library name: ENU54269B6G34_F3_neuro2081_S14_R1_001


title: ENU54269B6G32_F1_neuro2081_S14_R1_001

description: Library name: ENU54269B6G32_F1_neuro2081_S14_R1_001


title: ENU54272B6G310_B8_neuro2085_S50_R1_001

description: Library name: ENU54272B6G310_B8_neuro2085_S50_R1_001


title: ENU54272B6G310_B8_neuro2085_S42_R1_001

description: Library name: ENU54272B6G310_B8_neuro2085_S42_R1_001


title: ENU54269B6G316_F10_neuro2081_S14_R1_001

description: Library name: ENU54269B6G316_F10_neuro2081_S14_R1_001


title: ENU54269B6G33_F2_neuro2081_S14_R1_001

description: Library name: ENU54269B6G33_F2_neuro2081_S14_R1_001


title: ENU54269B6G318_F12_neuro2081_S14_R1_001

description: Library name: ENU54269B6G318_F12_neuro2081_S14_R1_001


title: ENU54269B6G315_F9_neuro2081_S14_R1_001

description: Library name: ENU54269B6G315_F9_neuro2081_S14_R1_001


title: ENU54266B6G35_A5_neuro2085_S49_R1_001

description: Library name: ENU54266B6G35_A5_neuro2085_S49_R1_001


title: ENU54269B6G37_F6_neuro2081_S14_R1_001

description: Library name: ENU54269B6G37_F6_neuro2081_S14_R1_001


title: ENU54266B6G36_A6_neuro2085_S49_R1_001

description: Library name: ENU54266B6G36_A6_neuro2085_S49_R1_001


title: ENU54266B6G34_A4_neuro2085_S41_R1_001

description: Library name: ENU54266B6G34_A4_neuro2085_S41_R1_001


title: ENU54272B6G312_B10_neuro2085_S50_R1_001

description: Library name: ENU54272B6G312_B10_neuro2085_S50_R1_001


title: ENU54269B6G317_F11_neuro2081_S14_R1_001

description: Library name: ENU54269B6G317_F11_neuro2081_S14_R1_001


title: ENU54266B6G34_A4_neuro2085_S49_R1_001

description: Library name: ENU54266B6G34_A4_neuro2085_S49_R1_001


title: ENU54266B6G32_A2_neuro2085_S49_R1_001

description: Library name: ENU54266B6G32_A2_neuro2085_S49_R1_001


description: Library name: ENU54267B6G36_D11_neuro2003rep_S92_R1_001


title: ENU54266B6G36_A6_neuro2085_S41_R1_001

description: Library name: ENU54266B6G36_A6_neuro2085_S41_R1_001


title: ENU54266B6G35_A5_neuro2085_S41_R1_001

description: Library name: ENU54266B6G35_A5_neuro2085_S41_R1_001


title: ENU54266B6G33_A3_neuro2085_S49_R1_001

description: Library name: ENU54266B6G33_A3_neuro2085_S49_R1_001


title: ENU54266B6G33_A3_neuro2085_S41_R1_001

description: Library name: ENU54266B6G33_A3_neuro2085_S41_R1_001


title: ENU54266B6G31_A1_neuro2085_S41_R1_001

description: Library name: ENU54266B6G31_A1_neuro2085_S41_R1_001


title: ENU54266B6G319_A12_neuro2085_S49_R1_001

description: Library name: ENU54266B6G319_A12_neuro2085_S49_R1_001


title: ENU54266B6G318_A11_neuro2085_S49_R1_001

description: Library name: ENU54266B6G318_A11_neuro2085_S49_R1_001


title: ENU54266B6G312_A9_neuro2085_S41_R1_001

description: Library name: ENU54266B6G312_A9_neuro2085_S41_R1_001


title: ENU54266B6G31_A1_neuro2085_S49_R1_001

description: Library name: ENU54266B6G31_A1_neuro2085_S49_R1_001


title: ENU54261B6G333_H12_neuro2084_S40_R1_001

description: Library name: ENU54261B6G333_H12_neuro2084_S40_R1_001


title: ENU54261B6G35_H5_neuro2084_S40_R1_001

description: Library name: ENU54261B6G35_H5_neuro2084_S40_R1_001


title: ENU54266B6G310_A7_neuro2085_S41_R1_001

description: Library name: ENU54266B6G310_A7_neuro2085_S41_R1_001


title: ENU54266B6G319_A12_neuro2085_S41_R1_001

description: Library name: ENU54266B6G319_A12_neuro2085_S41_R1_001


title: ENU54266B6G318_A11_neuro2085_S41_R1_001

description: Library name: ENU54266B6G318_A11_neuro2085_S41_R1_001


title: ENU54266B6G311_A8_neuro2085_S41_R1_001

description: Library name: ENU54266B6G311_A8_neuro2085_S41_R1_001


title: ENU54266B6G32_A2_neuro2085_S41_R1_001

description: Library name: ENU54266B6G32_A2_neuro2085_S41_R1_001


title: ENU54266B6G312_A9_neuro2085_S49_R1_001

description: Library name: ENU54266B6G312_A9_neuro2085_S49_R1_001


title: ENU54266B6G313_A10_neuro2085_S41_R1_001

description: Library name: ENU54266B6G313_A10_neuro2085_S41_R1_001


title: ENU54266B6G311_A8_neuro2085_S49_R1_001

description: Library name: ENU54266B6G311_A8_neuro2085_S49_R1_001


description: Library name: ENU54264B6G315_D10_neuro2003rep_S92_R1_001

value: Map4k5 W649L rerun


title: ENU54261B6G36_H6_neuro2084_S40_R1_001

description: Library name: ENU54261B6G36_H6_neuro2084_S40_R1_001


title: ENU54266B6G310_A7_neuro2085_S49_R1_001

description: Library name: ENU54266B6G310_A7_neuro2085_S49_R1_001


title: ENU54266B6G313_A10_neuro2085_S49_R1_001

description: Library name: ENU54266B6G313_A10_neuro2085_S49_R1_001


title: ENU54261B6G34_H4_neuro2084_S40_R1_001

description: Library name: ENU54261B6G34_H4_neuro2084_S40_R1_001


title: ENU54261B6G31_H1_neuro2084_S40_R1_001

description: Library name: ENU54261B6G31_H1_neuro2084_S40_R1_001


title: ENU54261B6G33_H3_neuro2084_S40_R1_001

description: Library name: ENU54261B6G33_H3_neuro2084_S40_R1_001


title: ENU54261B6G324_H9_neuro2084_S40_R1_001

description: Library name: ENU54261B6G324_H9_neuro2084_S40_R1_001


description: Library name: ENU54258B6G34_D8_neuro2003rep_S92_R1_001


title: ENU54249B6G314_C1_neuro2090_S83_R1_001

description: Library name: ENU54249B6G314_C1_neuro2090_S83_R1_001


title: ENU54249B6G317_C4_neuro2090_S83_R1_001

description: Library name: ENU54249B6G317_C4_neuro2090_S83_R1_001


title: ENU54249B6G316_C3_neuro2090_S83_R1_001

description: Library name: ENU54249B6G316_C3_neuro2090_S83_R1_001


title: ENU54241B6G37_G7_neuro2084_S39_R1_001

description: Library name: ENU54241B6G37_G7_neuro2084_S39_R1_001


title: ENU54261B6G331_H10_neuro2084_S40_R1_001

description: Library name: ENU54261B6G331_H10_neuro2084_S40_R1_001


title: ENU54249B6G315_C2_neuro2090_S83_R1_001

description: Library name: ENU54249B6G315_C2_neuro2090_S83_R1_001


title: ENU54241B6G322_G12_neuro2084_S39_R1_001

description: Library name: ENU54241B6G322_G12_neuro2084_S39_R1_001


title: ENU54241B6G321_G11_neuro2084_S39_R1_001

description: Library name: ENU54241B6G321_G11_neuro2084_S39_R1_001


title: ENU54241B6G31_G1_neuro2084_S39_R1_001

description: Library name: ENU54241B6G31_G1_neuro2084_S39_R1_001


description: Library name: ENU54214B6G322_C1_neuro2003rep_S91_R1_001


description: Library name: ENU54213B6G37_D6_neuro2003rep_S92_R1_001


description: Library name: ENU54209B6G319_E4_neuro2003rep_S93_R1_001


title: ENU54261B6G323_H8_neuro2084_S40_R1_001

description: Library name: ENU54261B6G323_H8_neuro2084_S40_R1_001


title: ENU54261B6G332_H11_neuro2084_S40_R1_001

description: Library name: ENU54261B6G332_H11_neuro2084_S40_R1_001


title: ENU54261B6G322_H7_neuro2084_S40_R1_001

description: Library name: ENU54261B6G322_H7_neuro2084_S40_R1_001


description: Library name: ENU54181B6G320_D3_neuro2003rep_S92_R1_001


description: Library name: ENU54181B6G319_D2_neuro2003rep_S92_R1_001


title: ENU54261B6G32_H2_neuro2084_S40_R1_001

description: Library name: ENU54261B6G32_H2_neuro2084_S40_R1_001


description: Library name: ENU54258B6G35_D9_neuro2003rep_S92_R1_001


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title: ENU54241B6G38_G8_neuro2084_S39_R1_001

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description: Library name: ENU53451B6G310_C9_neuro2039_S67_R1_001


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