title: NCI-H660-sgDNMT3B-3

city: Durham

first: Jung Wook

state: NC

address: 905 South Lasalle St.

department: Pathology

institute: Duke University

description: Library name: UUID_18

data_processing:
The sequencing libraries were multiplexed and clustered onto 3 flowcell lanes. After clustering, the flowcell was loaded on the Illumina HiSeq instrument (4000 or equivalent) according to manufacturer’s instructions. The samples were sequenced using a 2x150bp Paired End (PE) configuration. The HiSeq Control Software (HCS) conducted image analysis and base calling. Raw sequence data (.bcl files) generated from Illumina HiSeq was converted into fastq files and de-multiplexed using Illumina's bcl2fastq 2.20 software.
we used a pipeline developed at UCSC (UCSC Toil RNA-seq Recompute, https://xenabrowser.net/datapages/?hub=https://toil.xenahubs.net:443). CutAdapt was employed for adapter trimming, STAR for alignment, and RSEM and Kallisto served as quantifiers.
Assembly: hg18
Supplementary files format and content: normalized read counts

source_name: Prostate

growth_protocol: Growing in suspension (T75 flask) in B27-supplemented advanced DMEM/F12K media

extract_protocol:
RNeasy Mini Kit (Qiagen, Cat. #74104)
NEBNext Ultra II RNA Library Prep Kit for Illumina

treatment_protocol: NCI-H660 or MSKCC-EF1 cells were treated by sgDNMT3B or sgScrambe expressing lentiviruses

value: Prostate

value: Neuroendocrine prostate cancer cell line


title: mL_Rrp5_H375A _DMS_3

status: Public on Jul 30 2026

type: SRA

city: Nashville

first: zahrat el oula

last: hassoun

state: TN

address: 200 Robinson research

department: Biochemistry

country: USA

institute: Vanderbilt University

description: Library name: mL_H375A_DMS_rep3

library_source: transcriptomic

data_processing:
Adapter trimming and quality filtering were performed using Skewer. PCR duplicates were removed using SeqKit, and reads were trimmed at both ends. Processed reads were aligned to the Saccharomyces cerevisiae RDN37 pre_rRNA reference sequence using ShapeMapper v2.3.0 with Bowtie2 in DMS mode. Mutation frequencies were calculated using ShapeMapper after quality filtering. DMS_induced mutation frequencies were obtained by subtracting untreated from DMS_treated samples, and statistical analyses were performed using three biological replicates per condition.
Assembly: Saccharomyces cerevisiae RDN37 pre_rRNA reference sequence.
Supplementary files format and content: Tab_delimited text files containing per_nucleotide mutation frequencies and sequencing coverage.

molecule: total RNA

organism: Saccharomyces cerevisiae

source_name: Saccharomyces cerevisiae cell

growth_protocol: Saccharomyces cerevisiae cells were grown in YPD medium to mid_log phase before harvesting.

extract_protocol:
RNA was purified by phenol_chloroform extraction followed by ethanol precipitation.
RNA was fragmented, size_selected by denaturing polyacrylamide gel electrophoresis, dephosphorylated with T4 polynucleotide kinase, ligated to a pre_adenylated 3′ DNA adapter using truncated T4 RNA ligase 2 (K227Q), reverse transcribed using TGIRT_III reverse transcriptase, circularized with CircLigase II, amplified by indexed PCR, size_selected by native polyacrylamide gel electrophoresis, and quantified prior to sequencing. Libraries were sequenced on an Illumina NovaSeq X platform using single_end 100_bp reads.

treatment_protocol: Purified pre_40S ribosomes or mature 40S ribosomes were treated with 1% dimethyl sulfate (DMS) at 30°C for 5 min in the presence of 800 mM HEPES (pH 7.4), 500 mM NaCl, 50 mM Mg(OAc)2, and 0.2 μM Bacillus subtilis RNase P RNA. Untreated control samples were processed in parallel without DMS. Reactions were quenched with β_mercaptoethanol/NaOAc and RNA was purified by phenol_chloroform extraction.

tag: cell line

value: Saccharomyces cerevisiae cell


title: Vector_3

city: Fuzhou

first: Yiquan

last: Xu

address: Fujian Cancer Hospital

country: China

institute: Fujian meidical university

description: Library name: Vector_3

data_processing:
CLC Genomics Workbench v 11.0.1
Sequence reads were trimmed for adaptor sequence/low-quality sequence using CLC genomic benchwork (parameter- Quality limit: 0.05)
Trimmed sequence reads were mapped to hg38  using CLC genomic benchwork (parameters- mismath cost: 2 insertion cost: 3 deletion cost: 3 length fraction: 0.8 similarity fraction: 0.8)
Read count extraction and normalization were performed using CLC genomic benchwork
Assembly: hg38
Supplementary files format and content: tab-delimited text files include gene count and FPKM value for each Sample

organism: Homo sapiens

source_name: cell culture

extract_protocol:
RNA was extracted from cell culture using TRIzol reagent. Briefly, cell culture were washed with PBS, lysed in TRIzol, and incubated for 5 min. Chloroform (20% volume) was added, followed by vigorous mixing and centrifugation (12,000 ×g, 15 min, 4°C) to separate phases. The aqueous layer was collected, mixed with isopropanol, and centrifuged (12,000 ×g, 10 min) to precipitate RNA. The pellet was washed twice with 75% ethanol, air-dried, and dissolved in RNase-free water. RNA concentration and integrity (RIN >7) were assessed using Nanodrop and Bioanalyzer. Samples were stored at -80°C to prevent degradation. This method ensures high-quality RNA for transcriptomic analyses such as RNA-seq or qPCR.
Total RNA is enriched for small RNAs, followed by 3' and 5' adapter ligation. Reverse transcription generates cDNA, which is then PCR amplified with indexed primers. The final library is size-selected (typically ~150 bp) and validated by Bioanalyzer before sequencing on an Illumina flow cell.

tag: tissue

value: cell culture

tag: genotype

value: WT

tag: treatment


title: NCI-H660-sgDNMT3B-2

last: Park

description: Library name: UUID_17

tag: cell type


title: NCI-H660-sgDNMT3B-1

description: Library name: UUID_16


title: Vector_1

description: Library name: Vector_1


title: NDM_48h_rep1

type: RNA

city: Numazu

first: Takefumi

last: Onodera

address: 18-24 Miyamoto

department: Numazu

country: Japan

institute: Institute of microbial chemistry

description: Gene expression in PANC-1 cells after NDM treatment 48h

hyb_protocol: For each array, 1.65 µg of Cy3-labeled cRNA was fragmented according to the manufacturer’s one-color gene expression protocol and combined with Agilent gene expression hybridization buffer. The hybridization mixture was loaded onto the Agilent Whole Human Genome 4×44K v2 microarray slide and hybridized at 65°C for 17 h with rotating Agilent hybridization oven. After hybridization, slides were washed according to the manufacturer’s recommended one-color gene expression wash procedure and then dried prior to scanning.

scan_protocol: Slides were scanned on the Agilent DNA Microarray Scanner using one color scan setting for 4x44k array slides (Scan Area 61x21.6 mm, Scan resolution 5 um, Dye channel is set to Green and Green PMT is set to XDR Hi 100%, XDR Lo 10 %).

data_processing:
Feature Extraction output files were generated using Feature Extraction Software 9.5.3.1 (Agilent) with protocol GE1-v5_95 and grid 014850_D_20070820. Data were imported into GeneSpring GX, thresholded to 1.0, log2-transformed (log base 2), normalized across arrays by 75th percentile shift, and baseline-transformed to the median of all samples. Processed expression matrices were exported from GeneSpring GX.
DEG list generated in GeneSpring GX after re-normalization within the subset of samples used for this comparison.

source_name: Nutrient-deprived medium, 48h, replicate 1

label_protocol: Total RNA (200 ng per sample) was labeled using the Agilent Low Input Quick Amp Labeling Kit, One-Color (manufacturer’s protocol). Briefly, double-stranded cDNA was synthesized from total RNA using an oligo(dT)-T7 primer. Cy3-labeled cRNA was then generated by in vitro transcription with T7 RNA polymerase. Approximately 10 µg of cRNA was obtained per sample. Labeled cRNA was purified according to the kit protocol and quantified prior to hybridization.

extract_protocol: Total RNA was isolated using the RNeasy Mini Kit (Qiagen) following the manufacturer’s instructions. RNA was quantified using a NanoDrop-1000 spectrophotometer.

treatment_protocol: The gene expression profile under nutrient deprivation consisted of PANC-1 samples exposed to nutrient-deprived medium (NDM) for 0, 3, 6, 12, 24, and 48 h.

tag: condition

value: NDM treatment 48h


title: NDM_0h_rep4

description: Gene expression in PANC-1 cells after NDM treatment 0h

source_name: Nutrient-deprived medium, 0h, replicate 4

value: NDM treatment 0h


title: LCA__GAL LCA_GAL_1

city: Bethesda

first: David

last: McGaughey

state: MD

address: 10 Center Dr

department: NEI

institute: NIH

description:
Library name: LCA_GAL_1
dr_ciliopathy_treatments_cts.csv.gz

data_processing:
fastp fastq trimming
decoy aware salmon index
salmon quant --index  --libType A --seqBias --gcBias             --validateMappings
tximport into R (4.4)
gencode.v47.primary_assembly.annotation.gtf.gz
Assembly: GRCh38.primary_assembly.genome.fa.gz
Supplementary files format and content: counts, csv

molecule: polyA RNA

source_name: RPE

growth_protocol: All human samples were collected under an IRB-approved protocol at NEI (protocol #11-E1- 0245). Cells isolated from donor tissue were reprogrammed using Sendai virus-mediated delivery (CytoTune, LifeTechnologies) of the four Yamanaka factors (c-MYC, KLF4, OCT4, SOX2) following the manufacturer's recommendations. For differentiation, iPSCs were seeded onto vitronectin (ThermoFisher) coated T75 flasks followed by guided protocol. Triphasic developmentally guided protocol to generate retinal pigment epithelium from induced pluripotent stem cells, Sharma et. al. 2022

extract_protocol:
RNA was isolated from RPE monolayer 5 weeks post-seeding on transwells, using RNA extraction kit, Macherey&Nagel).
RNA libraries were prepared for sequencing using standard Illumina protocols

treatment_protocol: Samples were collected after 5 weeks of maturation on transwell inserts. Samples were either untreated or treated after 4days of seeding with Pioglitazone and 6days of seeding with Galunisetib.

value: RPE

value: iRPE

value: GAL


title: WT_Rrp5_H375A _DMS_3

description: Library name: WT_H375A_DMS_rep3


title: CTRL__PIO CTRL_PIO_3

description:
Library name: CTRL_PIO_3
dr_ciliopathy_treatments_cts.csv.gz

value: CTRL

value: PIO


title: NDM_24h_rep1

description: Gene expression in PANC-1 cells after NDM treatment 24h

source_name: Nutrient-deprived medium, 24h, replicate 1

value: NDM treatment 24h


title: KA09_SF_time_OP4_Control

city: Fort Collins

first: Steven

last: Dow

state: CO

address: 300 W Drake Rd

department: Clinical Sciences

institute: Colorado State University

description: Library name: KA09_SF_OP4

data_processing:
Differential analysis for comparisons between iPAAG treated and operated untreated controls was computed using Limma (Linear modeling and empirical Bayes) Voom (Mean–variance modeling) method (limma v3.62.2) longitudinal analysis with baseline (week 2 operated joints) with TMM normalization and duplicate Correlation() (block = patient) to estimate within-patient correlation
Fastp v0.23.2 was used to trim adapters and low-quality reads, STAR version 2.7.10b was used for alignment to Equus caballus EquCab3.0, annotated with Ensembl 113 and feature counts Version 2.0.1 was used to quantify reads.
Assembly: Equus caballus EquCab3.0
Supplementary files format and content: voom_logCPM_normalized_SF.csv

organism: Equus caballus

source_name: synovial fluid

growth_protocol: Synovial fluid samples (≥ 3 mL) were obtained at baseline (pre surgery) and every two weeks throughout study duration until end term (0, 2, 4, 6, 8, 10). To obtain synovial fluid samples, horses were sedated, and joints were aseptically prepared in routine fashion as described for treatment administration. Synovial fluid samples were collected under sterile conditions during routine arthrocentesis. Approximately 3 to 7 mL of synovial fluid were placed in a 15 mL conical tube and placed on ice for transport to the laboratory for processing.

extract_protocol:
The fluid was digested with 0.3 mg/mL hyaluronidase (Sigma, St. Louis, MO, USA) by incubation in a bead bath for 15 minutes at 37 ºC. Next the sample was centrifuged at 750 x g for 10 minutes at 8 ºC. The remaining cell pellet was washed with PBS and centrifuged at 500 x g for 10 minutes at 8 ºC. The cell pellet was resuspended in about 1 to 2 mL of PBS and transferred into a 2 mL microcentrifuge tube. The sample was then centrifuged for 500 x g for 15 minutes. The supernatant was discarded, and the cells were lysed in RLT buffer (Qiagen, Germantown, MD, USA) with 1% ß-Mercaptoethanol (BME; EMD Millipore Corp, Billerica, MA, USA) and stored at -80 ºC until RNA extraction.
Library preparation was conducted on synovial fluid samples using the SMARTer® Stranded Total RNA-Seq Kit -Pico input (Takara Bio USA, Inc.)

treatment_protocol: Treatment – Horses (n=8/group) were randomly assigned to receive either intra-articular 2.5% injectable polyacrylamide hydrogel (Arthramid®, Contura Vet, Franklin, TN, USA) injection (2 mL) or phosphate buffered saline (2 mL) injection. Briefly, horses were sedated with detomidine hydrochloride 0.01 mg/kg and butorphanol tartrate 0.01 mg/kg intravenously. The previously operated middle carpal joint was aseptically prepared in routine fashion and injected intra-articularly at day 14 of the study.

value: synovial fluid

value: SF cells


title: WT_Rrp5_H375A _DMS_2

description: Library name: WT_H375A_DMS_rep2


title: RNA-seq of N/TERT cells, O/A-treated, biol rep 4

city: Singapore

first: Yasunori

last: Saheki

address: 11 Mandalay Road

country: Singapore

institute: Lee Kong Chian School of Medicine, NTU

description: Library name: NT-OA-4

data_processing:
Obtained data were cleaned and aligned to reference genome (Homo sapiens, Ensembl, GRCh38.p13) via software Hisat2 (v2.0.1).
HTSeq (v0.6.1) was used to estimate gene and isoform expression levels from the pair-end clean data.
Finally, DESeq2 Bioconductor package was used to perform differential expression analysis.
The estimates of dispersion and logarithmic fold changes incorporate data-driven prior distributions, Padj of genes were set <= 0.05 to detect differential expressed ones.
Assembly: GRCh38.p13
Supplementary files format and content: all_fpkm_anno contains count and fpkm values for each sample
Supplementary files format and content: DE analysis_OA-Eth contains gene differential expression analysis of O/A vs Control

source_name: Cell line

growth_protocol: Keratinocytes (N/TERT-1 cells) (Gift from James G. Rheinwald) were cultured in Keratinocyte serum-free medium (KSFM) supplemented with 300 mM calcium chloride, human recombinant epidermal growth factor (rEGF), and bovine pituitary extract (BPE) as prepared according to manufacturer protocol, and 1% penicillin/streptomycin at 37°C and 5% carbon dioxide.

extract_protocol:
RNeasy mini kit (Qiagen) was used for the purification of total RNA from the cells.
1 μg total RNA was used for library preparation and subsequent mRNA-seq experiments. mRNA isolation was performed using Oligo(dT) beads, and the mRNA fragmentation was performed using divalent cations and high temperature. First strand cDNA and second-strand cDNA were synthesized using random primers. The purified double-stranded cDNA was then treated to repair both ends and add a dA-tailing in one reaction, followed by a T-A ligation to add adaptors to both ends. Size selection of Adaptor-ligated DNA was then performed using DNA Clean Beads. Each sample was then amplified by PCR using P5 and P7 primers and the PCR products were validated.

value: Cell line

value: epidermal keratinocyte


title: MSKCC-EF1-sgDNMT3B-2

description: Library name: UUID_14

value: Prostate


title: RNA-seq of N/TERT cells, O/A-treated, biol rep 3

description: Library name: NT-OA-3


title: NDM_0h_rep3

source_name: Nutrient-deprived medium, 0h, replicate 3


title: RNA-seq of N/TERT cells, O/A-treated, biol rep 2

description: Library name: NT-OA-2


title: NCI-H660-sgSCR-3

description: Library name: UUID_6

value: Neuroendocrine prostate cancer cell line

value: sgSCR


title: mL_Rrp5_H375A _noDMS_3

description: Library name: mL_H375A_noDMS_rep3

value: Saccharomyces cerevisiae cell


title: RNA-seq of N/TERT cells, O/A-treated, biol rep 1

description: Library name: NT-OA-1

value: WT


title: NDM_3h_rep2

description: Gene expression in PANC-1 cells after NDM treatment 3h

source_name: Nutrient-deprived medium, 3h, replicate 2

value: NDM treatment 3h


title: NCI-H660-sgSCR-2

description: Library name: UUID_5


title: mL_Rrp5_H375A _noDMS_2

description: Library name: mL_H375A_noDMS_rep2


title: NDM_12h_rep2

description: Gene expression in PANC-1 cells after NDM treatment 12h

source_name: Nutrient-deprived medium, 12h, replicate 2

value: NDM treatment 12h


title: RNA-seq of N/TERT cells, Ethanol-treated, biol rep 4

description: Library name: NT-Eth-4


title: NCI-H660-sgSCR-1

description: Library name: UUID_4


title: NDM_12h_rep1

source_name: Nutrient-deprived medium, 12h, replicate 1


title: mL_Rrp5_H375A _noDMS_1

description: Library name: mL_H375A_noDMS_rep1


title: RNA-seq of N/TERT cells, Ethanol-treated, biol rep 3

description: Library name: NT-Eth-3


title: NDM_6h_rep1

description: Gene expression in PANC-1 cells after NDM treatment 6h

source_name: Nutrient-deprived medium, 6h, replicate 1

value: NDM treatment 6h


title: MSKCC-EF1-sgSCR-3

description: Library name: UUID_3


title: WT_Rrp5_H375A_noDMS_3

description: Library name: WT_H375A_noDMS_rep3


title: RNA-seq of N/TERT cells, Ethanol-treated, biol rep 2

description: Library name: NT-Eth-2


title: KA08_SF_time_OP2_iPAAG

description: Library name: KA08_SF_OP2


title: CEP290__GAL CEP290_GAL_3

description:
Library name: CEP290_GAL_3
dr_ciliopathy_treatments_cts.csv.gz

value: RPE

value: iRPE


title: MSKCC-EF1-sgSCR-2

description: Library name: UUID_2


title: RNA-seq of N/TERT cells, Ethanol-treated, biol rep 1

description: Library name: NT-Eth-1


title: E37_3_VZ

status: Public on Jul 29 2026

city: Kunming

first: lei

last: shi

state: Yunnan

address: No.17 Longxin Road

institute: Kunming Institute of Zoology

data_processing:
Illumina  software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to Chinese_treeshrew_genome_2.0(http://www.treeshrewdb.org/)whole genome using Hisat2
Fragments per kilobase of transcript per million mapped fragments (FPKM) were calculated. First step, align RNA seq reads to the genome by using  Hista2 ; and then Cufflinks2 will assemble transcripts and computed the FPKM vlues(http://cufflinks.cbcb.umd.edu/),following protocol from Pertea, M.et al  Nat Protoc 11, 1650-1667, (2016).
Assembly: Chinese_treeshrew_genome_2.0
Supplementary files format and content: tab-delimited text files include FPKM values for each Sample

organism: Tupaia belangeri chinensis

source_name: Fetal cortex

extract_protocol:
The fetal tree shrew brain tissues were partitioned into several sections, after that, the specimens were embedded in OCT and then gradually frozen in an ethnol /dry ice, and stored at −80°C until processing. Frozen tissue was cryosectioned at 30μm onto polyethylene naphthalate (PEN) slides (Leica Microsystems) and place immediately into microslide box on dry ice, and stored at -80°C until use.    Taking the section slides from the -80°C freezer and put them on a blotting paper for 30s, and then transfer the slides into the 95% EtOH for 30s, 75% EtOH for 30s and 50% EtOH for 30s, place the slides on a blotting paper. Add 400ul Nissl stain solution on the slide, and incubate at room temperature for 40s. Proceed through the dehydration series (50%, 75% and 95% EtOH) till the two rounds of 100% EtOH, and leave the slides in the final xylene jar for 5 min. After air-drying in the fume hood for 5 min, proceed immediately with laser capture microdissection (LCM).   Laser microdissection was performed on a Leica LMD6000 (Leica Microsystems). Microdissected tissues including CP, OSVZ, SVZ and VZ were collected directly into QIAzol buffer from the miRNeasy Mini Kit (Qiagen) in a 0.2ml tubes, vortexed, centrifuged, and frozen at −80 °C. RNA was isolated following the manufacturer’s directions for the miRNeasy Mini Kit. RNA samples were run on the Agilent 2100 Bioanalyzer (Agilent Technologies).
RNA sequencing library construction followed VAHTs mRNA-seq V3 Library Prep Kit for Illumina (Vazyme, #NR611). The total RNA input was 0.7-1ug, mRNA (with ploy-A) binds to mRNA capture beads (with Oligo dT), mRNA is eluted from beads and then fragmented, synthesis of the 1st strand of cDNA using random primers, synthesis of the 2nd strand of cDNA after RNase H digestion. Size selection and library amplication after end repair and adapter ligation, the final RNA sequencing library length is between 320-420bp. Library quality determination analyzed by an Agilent Technologies 2100 Bioanalyzer.

value: Fetal cortex

tag: layer

value: Ventricular Zone

tag: age

value: Embryonic 37 days


title: NDM_0h_rep2

source_name: Nutrient-deprived medium, 0h, replicate 2

value: NDM treatment 0h


title: NDM_0h_rep1

source_name: Nutrient-deprived medium, 0h, replicate 1


title: WT_Rrp5_H375A_noDMS_1

description: Library name: WT_H375A_noDMS_rep1


title: E37_3_ISVZ

value: Inner Subventricular Zone


title: E37_3_CP

value: Cortical plate


title: E37_2_VZ


title: E37_2_OSVZ

value: Outer Subventricular Zone


title: E37_2_ISVZ


title: E37_1_VZ


title: E37_2_CP


title: E37_1_OSVZ


title: E37_1_ISVZ


title: E37_1_CP


title: Adipocyte ERR-NanoDam, male, replicate 3

city: Bloomington

first: Lesley

middle: Nicole

last: Weaver

state: IN

address: 1001 E. 3rd Street

department: Biology

institute: Indiana University

description: Library name: Sample 15

library_source: genomic

data_processing:
Samples were trimmed for quality and adapters using fastp v0.21.0 using default parameters
The trimmed reads were then mapped with bowtie2 v2.5.4 (non-default parameters: --local) to the Drosophila genome (r6.42) in local mode to exclude special adapters that were not efficiently removed during library preparation.
Counts were determined by identifying properly paired inserts that spanned a region less than 1,000 bps; any DpnI restriction fragment that the insert overlapped was incremented by one. The resulting counts table was analyzed using the R language package DESeq2 to identify differentially abundant DpnI fragments between the experimental and control samples.
Assembly: ensembl_drosophila_melanogaster_bdgp6_gca_000001215_4
Supplementary files format and content: Excel files contains all regions identified by ERR-NanoDam in male and female adipocytes
Library strategy: DamID-Seq

molecule: genomic DNA

organism: Drosophila melanogaster

source_name: Adipocytes

extract_protocol:
Whole body samples were flash frozen in liquid nitrogen and genomic DNA was isolated using the Qiagen Purgene Tissue Kit according to manufacturer's instructions.
Methylated DNA enrichment and library preparation were carried out as previous described (Tang et al 2022) with slight modifications.

value: Adipocytes


title: Adipocyte ERR-NanoDam, male, replicate 2

description: Library name: Sample 14


title: Adipocyte ERR-NanoDam, male, replicate 4

description: Library name: Sample 16


title: Adipocyte ERR-NanoDam, male, replicate 1

description: Library name: Sample 13


title: Adipocyte NanoDam control, male, replicate 4

description: Library name: Sample 12


title: Adipocyte NanoDam control, male, replicate 3

description: Library name: Sample 11


title: Adipocyte NanoDam control, male, replicate 2

description: Library name: Sample 10


title: Adipocyte NanoDam control, male, replicate 1

description: Library name: Sample 9


title: Adipocyte ERR-NanoDam, female, replicate 3

description: Library name: Sample 7


title: Adipocyte ERR-NanoDam, female, replicate 4

description: Library name: Sample 8


title: Adipocyte NanoDam control, female, replicate 2

description: Library name: Sample 2


title: Adipocyte NanoDam control, female, replicate 4

description: Library name: Sample 4


title: Adipocyte NanoDam control, female, replicate 3

description: Library name: Sample 3


title: Adipocyte ERR-NanoDam, female, replicate 1

description: Library name: Sample 5


title: SUB23, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day30

city: Rockville

first: Johannes

last: Goll

address: 401 N Washington St

department: Bioinformatics Service Group

institute: The Emmes Company

description:
Library name: SUB23_D30_MNR
SUB23_D30_MNR

data_processing:
Human reference genome assembly (GRCh38), gene models, and associated gene annotation information were obtained from the ENSEMBL database (Version 99). The genomic reference was built by merging all human chromosomes except for X and Y chromosomes (to avoid gender-specific effects). Adapter sequences and low-quality 5′ ending sequences were removed from raw sequencing reads using the Trimmomatic software (Version 0.39). Following adapter removal, sequence reads were aligned to the reference transcriptome/genome using HISAT2 splice-aware sequence aligner (Version 2.2.0).  For each sample, the quality of reference alignments was evaluated using the RSeQC software (Version 3.0.1). Gene expression quantification was conducted on the gene level using Subread v2.0.0 counting mapped paired-reads to obtain fragment counts per gene.
Assembly: GRCh38 ENSEMBL database (Version 99)
Supplementary files format and content: tab-delimited text includes raw counts of each ENSEMBL Gene ID for each Sample

source_name: Monocytes

extract_protocol:
Peripheral blood mononuclear cells (PBMC) were recovered from phlebotomy samples and processed immediately. Magnetic sorting (AutoMACS Pro) was used to isolate individual immune cells using microbeads coated with CD15 (PMN), CD19 (B-cells), CD3 (T-cells), CD56 (NK cells), CD11c (dendritic cells), and CD14 (monocytes). During optimization, flow cytometric analysis confirmed >99% purity of each immune cell compartment. RNA was extracted from PBMC using the Promega simplyRNA Cell Kit according to the manufacturer’s protocol.
Polyadenylated RNAs were captured using NEBNext Magnetic Oligo d(T)25 Beads, and the NEBNext mRNA Library Prep Reagent Set for Illumina (New England BioLabs Inc., Ipswich, MA, USA) was used to prepare individually bar-coded libraries as per manufacturer’s recommended protocol. Accurate quantification for sequencing applications was determined using the qPCR-based KAPA Biosystems Library Quantification Kit (Kapa Biosystems, Inc., Woburn, MA, USA).
Paired-end sequencing (25 million, 75-bp, paired-end reads) was performed on an Illumina Novaseq 6000 sequencers (Illumina, Inc., San Diego, CA, USA) as per manufacturer’s recommended protocol.

value: Monocytes

value: A/H7N9 vaccine with AS03 adjuvant

tag: day


title: SUB23, B cells, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB23_D30_BCR
SUB23_D30_BCR

source_name: B cells

value: B cells


title: SUB23, T cells, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB23_D2_TCR
SUB23_D2_TCR

source_name: T cells

value: T cells


title: SUB23, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB23_D30_DNR
SUB23_D30_DNR

source_name: Dendritic cells

value: Dendritic cells


title: Adipocyte NanoDam control, female, replicate 1

description: Library name: Sample 1


title: SUB23, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB23_D2_NKR
SUB23_D2_NKR

source_name: NK cells

value: NK cells


title: SUB23, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB23_D2_MNR
SUB23_D2_MNR


title: NDM_6h_rep2

source_name: Nutrient-deprived medium, 6h, replicate 2

value: NDM treatment 6h


title: SUB23, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB23_D2_DNR
SUB23_D2_DNR


title: SUB23, B cells, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB23_D2_BCR
SUB23_D2_BCR


title: SUB23, T cells, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB23_D29_TCR
SUB23_D29_TCR


title: SUB23, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB23_D29_NKR
SUB23_D29_NKR


title: SUB23, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB23_D29_MNR
SUB23_D29_MNR


title: SUB23, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB23_D29_NER
SUB23_D29_NER

source_name: Neutrophils

value: Neutrophils


title: SUB23, T cells, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB23_D1_TCR
SUB23_D1_TCR


title: SUB23, B cells, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB23_D29_BCR
SUB23_D29_BCR


title: SUB23, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB23_D1_NKR
SUB23_D1_NKR


title: SUB23, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB23_D1_NER
SUB23_D1_NER


title: SUB23, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB23_D29_DNR
SUB23_D29_DNR


title: SUB23, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB23_D1_MNR
SUB23_D1_MNR


title: SUB23, B cells, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB23_D1_BCR
SUB23_D1_BCR


title: SUB23, T cells, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB23_D.7_TCR
SUB23_D.7_TCR


title: SUB23, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB23_D1_DNR
SUB23_D1_DNR


title: SUB23, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB23_D.7_NKR
SUB23_D.7_NKR


title: SUB23, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB23_D.7_MNR
SUB23_D.7_MNR


title: SUB23, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB23_D.7_NER
SUB23_D.7_NER


title: SUB23, B cells, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB23_D.7_BCR
SUB23_D.7_BCR


title: SUB22, NK cells, A/H3N2, Day8

description:
Library name: SUB22_D8_NKR
SUB22_D8_NKR


title: SUB22, T cells, A/H3N2, Day8

description:
Library name: SUB22_D8_TCR
SUB22_D8_TCR


title: SUB22, Monocytes, A/H3N2, Day8

description:
Library name: SUB22_D8_MNR
SUB22_D8_MNR


title: SUB22, Dendritic cells, A/H3N2, Day8

description:
Library name: SUB22_D8_DNR
SUB22_D8_DNR


title: SUB22, T cells, A/H3N2, Day4

description:
Library name: SUB22_D4_TCR
SUB22_D4_TCR


title: SUB22, Monocytes, A/H3N2, Day4

description:
Library name: SUB22_D4_MNR
SUB22_D4_MNR


title: SUB22, Neutrophils, A/H3N2, Day4

description:
Library name: SUB22_D4_NER
SUB22_D4_NER


title: SUB22, B cells, A/H3N2, Day4

description:
Library name: SUB22_D4_BCR
SUB22_D4_BCR


title: SUB22, NK cells, A/H3N2, Day2

description:
Library name: SUB22_D2_NKR
SUB22_D2_NKR


title: SUB22, T cells, A/H3N2, Day2

description:
Library name: SUB22_D2_TCR
SUB22_D2_TCR


title: SUB22, Neutrophils, A/H3N2, Day2

description:
Library name: SUB22_D2_NER
SUB22_D2_NER


title: SUB22, Monocytes, A/H3N2, Day2

description:
Library name: SUB22_D2_MNR
SUB22_D2_MNR


title: SUB22, B cells, A/H3N2, Day2

description:
Library name: SUB22_D2_BCR
SUB22_D2_BCR


title: SUB22, T cells, A/H3N2, Day1

description:
Library name: SUB22_D1_TCR
SUB22_D1_TCR


title: SUB22, NK cells, A/H3N2, Day1

description:
Library name: SUB22_D1_NKR
SUB22_D1_NKR


title: SUB22, Monocytes, A/H3N2, Day1

description:
Library name: SUB22_D1_MNR
SUB22_D1_MNR


title: SUB22, B cells, A/H3N2, Day1

description:
Library name: SUB22_D1_BCR
SUB22_D1_BCR


title: SUB22, NK cells, A/H3N2, Day4

description:
Library name: SUB22_D4_NKR
SUB22_D4_NKR


title: SUB22, NK cells, A/H3N2, Day-7

description:
Library name: SUB22_D.7_NKR
SUB22_D.7_NKR


title: SUB22, Neutrophils, A/H3N2, Day-7

description:
Library name: SUB22_D.7_NER
SUB22_D.7_NER


title: SUB22, Monocytes, A/H3N2, Day-7

description:
Library name: SUB22_D.7_MNR
SUB22_D.7_MNR


title: SUB22, Dendritic cells, A/H3N2, Day1

description:
Library name: SUB22_D1_DNR
SUB22_D1_DNR


title: SUB22, Dendritic cells, A/H3N2, Day-7

description:
Library name: SUB22_D.7_DNR
SUB22_D.7_DNR


title: SUB21, Neutrophils, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB21_D4_NER
SUB21_D4_NER

value: A/H7N9 vaccine unadjuvanted


title: SUB21, T cells, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB21_D4_TCR
SUB21_D4_TCR


title: SUB21, NK cells, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB21_D4_NKR
SUB21_D4_NKR


title: SUB21, Monocytes, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB21_D4_MNR
SUB21_D4_MNR


title: SUB21, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB21_D4_DNR
SUB21_D4_DNR


title: SUB21, B cells, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB21_D4_BCR
SUB21_D4_BCR


title: SUB21, T cells, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB21_D36_TCR
SUB21_D36_TCR


title: SUB23, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB23_D.7_DNR
SUB23_D.7_DNR


title: SUB21, Neutrophils, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB21_D36_NER
SUB21_D36_NER


title: SUB22, B cells, A/H3N2, Day8

description:
Library name: SUB22_D8_BCR
SUB22_D8_BCR


title: SUB21, Monocytes, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB21_D36_MNR
SUB21_D36_MNR


title: SUB22, Dendritic cells, A/H3N2, Day4

description:
Library name: SUB22_D4_DNR
SUB22_D4_DNR


title: SUB21, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB21_D36_DNR
SUB21_D36_DNR


title: SUB21, B cells, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB21_D36_BCR
SUB21_D36_BCR


title: SUB21, T cells, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB21_D32_TCR
SUB21_D32_TCR


title: SUB21, NK cells, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB21_D32_NKR
SUB21_D32_NKR


title: SUB21, Neutrophils, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB21_D32_NER
SUB21_D32_NER


title: SUB22, Dendritic cells, A/H3N2, Day2

description:
Library name: SUB22_D2_DNR
SUB22_D2_DNR


title: SUB21, Monocytes, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB21_D32_MNR
SUB21_D32_MNR


title: mL_Rrp5_H375A _DMS_2

description: Library name: mL_H375A_DMS_rep2

source_name: Saccharomyces cerevisiae cell

value: Saccharomyces cerevisiae cell


title: Vector_2

description: Library name: Vector_2

source_name: cell culture

value: cell culture

value: WT


title: mL_Rrp5_H375A _DMS_1

description: Library name: mL_H375A_DMS_rep1


title: SUB21, B cells, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB21_D32_BCR
SUB21_D32_BCR


title: SUB21, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB21_D32_DNR
SUB21_D32_DNR


title: SUB21, T cells, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB21_D30_TCR
SUB21_D30_TCR


title: SUB21, NK cells, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB21_D30_NKR
SUB21_D30_NKR


title: SUB22, Neutrophils, A/H3N2, Day1

description:
Library name: SUB22_D1_NER
SUB22_D1_NER


title: MSKCC-EF1-sgDNMT3B-3

description: Library name: UUID_15

source_name: Prostate

value: Prostate

value: Neuroendocrine prostate cancer cell line


title: SUB21, Neutrophils, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB21_D30_NER
SUB21_D30_NER


title: SUB21, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB21_D30_DNR
SUB21_D30_DNR


title: SUB21, Monocytes, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB21_D30_MNR
SUB21_D30_MNR


title: MSKCC-EF1-sgDNMT3B-1

description: Library name: UUID_13


title: WT_Rrp5_H375A _DMS_1

description: Library name: WT_H375A_DMS_rep1


title: SUB22, T cells, A/H3N2, Day-7

description:
Library name: SUB22_D.7_TCR
SUB22_D.7_TCR


title: SUB21, Neutrophils, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB21_D2_NER
SUB21_D2_NER


title: SUB21, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB21_D2_DNR
SUB21_D2_DNR


title: SUB21, T cells, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB21_D2_TCR
SUB21_D2_TCR


title: SUB21, NK cells, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB21_D2_NKR
SUB21_D2_NKR


title: SUB21, Monocytes, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB21_D2_MNR
SUB21_D2_MNR


title: SUB21, T cells, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB21_D29_TCR
SUB21_D29_TCR


title: SUB21, B cells, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB21_D2_BCR
SUB21_D2_BCR


title: SUB21, NK cells, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB21_D29_NKR
SUB21_D29_NKR


title: SUB21, Neutrophils, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB21_D29_NER
SUB21_D29_NER


title: SUB21, Monocytes, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB21_D29_MNR
SUB21_D29_MNR


title: SUB21, B cells, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB21_D30_BCR
SUB21_D30_BCR


title: SUB21, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB21_D29_DNR
SUB21_D29_DNR


title: SUB21, Neutrophils, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB21_D1_NER
SUB21_D1_NER


title: SUB21, NK cells, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB21_D1_NKR
SUB21_D1_NKR


title: SUB21, Monocytes, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB21_D1_MNR
SUB21_D1_MNR


title: SUB21, B cells, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB21_D1_BCR
SUB21_D1_BCR


title: SUB21, T cells, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB21_D1_TCR
SUB21_D1_TCR


title: SUB21, NK cells, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB21_D.7_NKR
SUB21_D.7_NKR


title: SUB21, T cells, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB21_D.7_TCR
SUB21_D.7_TCR


title: SUB21, B cells, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB21_D.7_BCR
SUB21_D.7_BCR


title: SUB21, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB21_D.7_DNR
SUB21_D.7_DNR


title: SUB21, Monocytes, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB21_D.7_MNR
SUB21_D.7_MNR


title: SUB20, T cells, A/H3N2, Day8

description:
Library name: SUB20_D8_TCR
SUB20_D8_TCR


title: SUB20, NK cells, A/H3N2, Day8

description:
Library name: SUB20_D8_NKR
SUB20_D8_NKR


title: SUB20, Neutrophils, A/H3N2, Day8

description:
Library name: SUB20_D8_NER
SUB20_D8_NER


title: SUB21, Neutrophils, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB21_D.7_NER
SUB21_D.7_NER


title: SUB20, Dendritic cells, A/H3N2, Day8

description:
Library name: SUB20_D8_DNR
SUB20_D8_DNR


title: SUB20, B cells, A/H3N2, Day8

description:
Library name: SUB20_D8_BCR
SUB20_D8_BCR


title: SUB20, T cells, A/H3N2, Day4

description:
Library name: SUB20_D4_TCR
SUB20_D4_TCR


title: SUB20, NK cells, A/H3N2, Day4

description:
Library name: SUB20_D4_NKR
SUB20_D4_NKR


title: SUB20, Monocytes, A/H3N2, Day4

description:
Library name: SUB20_D4_MNR
SUB20_D4_MNR


title: SUB20, B cells, A/H3N2, Day4

description:
Library name: SUB20_D4_BCR
SUB20_D4_BCR


title: SUB20, NK cells, A/H3N2, Day2

description:
Library name: SUB20_D2_NKR
SUB20_D2_NKR


title: SUB20, T cells, A/H3N2, Day2

description:
Library name: SUB20_D2_TCR
SUB20_D2_TCR


title: SUB20, Neutrophils, A/H3N2, Day2

description:
Library name: SUB20_D2_NER
SUB20_D2_NER


title: SUB20, Dendritic cells, A/H3N2, Day4

description:
Library name: SUB20_D4_DNR
SUB20_D4_DNR


title: SUB20, Dendritic cells, A/H3N2, Day2

description:
Library name: SUB20_D2_DNR
SUB20_D2_DNR


title: SUB20, Monocytes, A/H3N2, Day2

description:
Library name: SUB20_D2_MNR
SUB20_D2_MNR


title: SUB20, B cells, A/H3N2, Day2

description:
Library name: SUB20_D2_BCR
SUB20_D2_BCR


title: SUB20, T cells, A/H3N2, Day1

description:
Library name: SUB20_D1_TCR
SUB20_D1_TCR


title: SUB20, NK cells, A/H3N2, Day1

description:
Library name: SUB20_D1_NKR
SUB20_D1_NKR


title: SUB20, Monocytes, A/H3N2, Day1

description:
Library name: SUB20_D1_MNR
SUB20_D1_MNR


title: SUB20, Neutrophils, A/H3N2, Day1

description:
Library name: SUB20_D1_NER
SUB20_D1_NER


title: SUB20, Dendritic cells, A/H3N2, Day1

description:
Library name: SUB20_D1_DNR
SUB20_D1_DNR


title: SUB20, B cells, A/H3N2, Day1

description:
Library name: SUB20_D1_BCR
SUB20_D1_BCR


title: SUB20, Neutrophils, A/H3N2, Day-7

description:
Library name: SUB20_D.7_NER
SUB20_D.7_NER


title: SUB20, NK cells, A/H3N2, Day-7

description:
Library name: SUB20_D.7_NKR
SUB20_D.7_NKR


title: SUB20, Monocytes, A/H3N2, Day-7

description:
Library name: SUB20_D.7_MNR
SUB20_D.7_MNR


title: SUB20, T cells, A/H3N2, Day-7

description:
Library name: SUB20_D.7_TCR
SUB20_D.7_TCR


title: SUB20, Dendritic cells, A/H3N2, Day-7

description:
Library name: SUB20_D.7_DNR
SUB20_D.7_DNR


title: SUB19, T cells, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB19_D4_TCR
SUB19_D4_TCR


title: SUB20, B cells, A/H3N2, Day-7

description:
Library name: SUB20_D.7_BCR
SUB20_D.7_BCR


title: SUB19, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB19_D4_NKR
SUB19_D4_NKR


title: SUB19, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB19_D4_NER
SUB19_D4_NER


title: SUB19, B cells, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB19_D4_BCR
SUB19_D4_BCR


title: SUB19, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB19_D4_MNR
SUB19_D4_MNR


title: SUB19, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB19_D4_DNR
SUB19_D4_DNR


title: SUB19, T cells, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB19_D36_TCR
SUB19_D36_TCR


title: SUB19, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB19_D36_NER
SUB19_D36_NER


title: SUB19, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB19_D36_MNR
SUB19_D36_MNR


title: SUB19, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB19_D36_DNR
SUB19_D36_DNR


title: SUB19, B cells, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB19_D36_BCR
SUB19_D36_BCR


title: SUB19, T cells, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB19_D32_TCR
SUB19_D32_TCR


title: SUB19, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB19_D32_NER
SUB19_D32_NER


title: SUB19, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB19_D32_DNR
SUB19_D32_DNR


title: SUB19, B cells, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB19_D32_BCR
SUB19_D32_BCR


title: SUB19, T cells, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB19_D30_TCR
SUB19_D30_TCR


title: SUB19, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB19_D30_NKR
SUB19_D30_NKR


title: SUB19, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB19_D30_MNR
SUB19_D30_MNR


title: SUB19, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB19_D30_DNR
SUB19_D30_DNR


title: SUB19, B cells, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB19_D30_BCR
SUB19_D30_BCR


title: SUB19, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB19_D32_NKR
SUB19_D32_NKR


title: SUB19, T cells, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB19_D2_TCR
SUB19_D2_TCR


title: SUB19, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB19_D2_NKR
SUB19_D2_NKR


title: SUB19, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB19_D2_NER
SUB19_D2_NER


title: SUB19, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB19_D2_DNR
SUB19_D2_DNR


title: SUB19, B cells, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB19_D2_BCR
SUB19_D2_BCR


title: SUB19, T cells, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB19_D29_TCR
SUB19_D29_TCR


title: SUB19, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB19_D29_NKR
SUB19_D29_NKR


title: SUB19, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB19_D29_NER
SUB19_D29_NER


title: SUB19, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB19_D29_MNR
SUB19_D29_MNR


title: SUB19, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB19_D29_DNR
SUB19_D29_DNR


title: SUB19, B cells, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB19_D29_BCR
SUB19_D29_BCR


title: SUB19, T cells, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB19_D1_TCR
SUB19_D1_TCR


title: SUB19, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB19_D1_NKR
SUB19_D1_NKR


title: SUB19, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB19_D1_MNR
SUB19_D1_MNR


title: SUB19, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB19_D1_NER
SUB19_D1_NER


title: SUB19, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB19_D1_DNR
SUB19_D1_DNR


title: SUB19, T cells, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB19_D.7_TCR
SUB19_D.7_TCR


title: SUB19, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB19_D.7_MNR
SUB19_D.7_MNR


title: SUB19, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB19_D.7_NER
SUB19_D.7_NER


title: SUB19, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB19_D.7_DNR
SUB19_D.7_DNR


title: SUB19, B cells, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB19_D.7_BCR
SUB19_D.7_BCR


title: SUB18, T cells, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB18_D4_TCR
SUB18_D4_TCR


title: SUB18, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB18_D4_DNR
SUB18_D4_DNR


title: SJMEL063827_X1

city: Memphis

first: Michael

last: Dyer

address: 262 Danny Thomas Pl MS 324

department: Developmental Neurobiology

institute: St. Jude Children's Research Hospital

description:
Library name: SJMEL063827_X1
10X Genomics 3’ RNA kit

library_source: transcriptomic single cell

data_processing:
Aligning and transcript counting were performed using 10x Genomics Cell Ranger version 7
Assembly: Hg38 for patient samples. Combined Hg38 and mm10 for PDX samples
Supplementary files format and content: processed data reflect cell ranger output from the filter_bc_matrix folder, including feature names, cell barcode IDs, and expression count matrix (unnormalized)

source_name: MEL

extract_protocol:
cells or nuclei were extracting using protocols described in Slyper et al Nat Medicine 2020 (https://doi.org/10.1038/s41591-020-0844-1)
Libraries were generating using the 10x Genomics 3' gene expression kits version 3.1

value: MEL

tag: group

value: O-PDX

tag: method

value: nucleus

tag: sjid

tag: gender

value: female

tag: primary site

value: pontine meninges

tag: sample site

tag: timepoint

value: diagnostic

tag: sample

value: primary

tag: treatment status

value: treated


title: SUB18, T cells, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB18_D36_TCR
SUB18_D36_TCR

tag: tissue

value: T cells


title: SUB18, Neutrophils, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB18_D36_NER
SUB18_D36_NER


title: SUB18, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB18_D36_DNR
SUB18_D36_DNR

value: Dendritic cells


title: SUB18, Monocytes, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB18_D36_MNR
SUB18_D36_MNR

value: Monocytes


title: SUB18, B cells, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB18_D36_BCR
SUB18_D36_BCR

value: B cells

value: A/H7N9 vaccine unadjuvanted


title: SUB18, NK cells, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB18_D32_NKR
SUB18_D32_NKR

value: NK cells


title: SUB18, Neutrophils, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB18_D32_NER
SUB18_D32_NER

value: Neutrophils


title: SUB18, Monocytes, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB18_D32_MNR
SUB18_D32_MNR


title: SUB18, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB18_D32_DNR
SUB18_D32_DNR


title: SUB18, B cells, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB18_D32_BCR
SUB18_D32_BCR


title: SUB18, T cells, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB18_D30_TCR
SUB18_D30_TCR


title: SUB18, NK cells, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB18_D30_NKR
SUB18_D30_NKR


title: SUB18, Neutrophils, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB18_D30_NER
SUB18_D30_NER


title: SUB18, Monocytes, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB18_D30_MNR
SUB18_D30_MNR


title: SUB18, B cells, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB18_D30_BCR
SUB18_D30_BCR


title: SUB18, T cells, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB18_D2_TCR
SUB18_D2_TCR


title: SUB18, NK cells, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB18_D2_NKR
SUB18_D2_NKR


title: SUB19, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB19_D36_NKR
SUB19_D36_NKR

value: A/H7N9 vaccine with AS03 adjuvant


title: SUB18, Neutrophils, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB18_D2_NER
SUB18_D2_NER


title: SUB19, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB19_D32_MNR
SUB19_D32_MNR


title: SUB19, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB19_D30_NER
SUB19_D30_NER


title: SUB18, Monocytes, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB18_D2_MNR
SUB18_D2_MNR


title: SUB18, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB18_D2_DNR
SUB18_D2_DNR


title: SUB18, B cells, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB18_D2_BCR
SUB18_D2_BCR


title: SUB19, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB19_D2_MNR
SUB19_D2_MNR


title: SUB18, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB18_D30_DNR
SUB18_D30_DNR


title: SUB18, T cells, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB18_D29_TCR
SUB18_D29_TCR


title: SUB18, NK cells, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB18_D29_NKR
SUB18_D29_NKR


title: SUB18, Neutrophils, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB18_D29_NER
SUB18_D29_NER


title: E37_3_OSVZ

value: Fetal cortex

value: Outer Subventricular Zone

value: Embryonic 37 days


title: SUB18, Monocytes, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB18_D29_MNR
SUB18_D29_MNR


title: SUB18, B cells, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB18_D29_BCR
SUB18_D29_BCR


title: SUB18, T cells, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB18_D1_TCR
SUB18_D1_TCR


title: SUB18, NK cells, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB18_D1_NKR
SUB18_D1_NKR


title: SUB18, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB18_D29_DNR
SUB18_D29_DNR


title: SUB18, Neutrophils, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB18_D1_NER
SUB18_D1_NER


title: SUB19, B cells, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB19_D1_BCR
SUB19_D1_BCR


title: SUB18, Monocytes, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB18_D1_MNR
SUB18_D1_MNR


title: SUB18, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB18_D1_DNR
SUB18_D1_DNR


title: SUB19, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB19_D.7_NKR
SUB19_D.7_NKR


title: SUB18, T cells, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB18_D.7_TCR
SUB18_D.7_TCR


title: SUB18, B cells, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB18_D1_BCR
SUB18_D1_BCR


title: SUB18, Neutrophils, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB18_D.7_NER
SUB18_D.7_NER


title: Adipocyte ERR-NanoDam, female, replicate 2

description: Library name: Sample 6

value: Adipocytes


title: SUB18, Monocytes, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB18_D.7_MNR
SUB18_D.7_MNR


title: SUB18, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB18_D.7_DNR
SUB18_D.7_DNR


title: SUB18, NK cells, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB18_D4_NKR
SUB18_D4_NKR


title: SUB18, Neutrophils, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB18_D4_NER
SUB18_D4_NER


title: SUB18, B cells, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB18_D.7_BCR
SUB18_D.7_BCR


title: SUB17, T cells, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB17_D4_TCR
SUB17_D4_TCR


title: SUB17, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB17_D4_NKR
SUB17_D4_NKR


title: SUB17, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB17_D4_MNR
SUB17_D4_MNR


title: SUB17, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB17_D4_DNR
SUB17_D4_DNR


title: SUB18, Monocytes, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB18_D4_MNR
SUB18_D4_MNR


title: SUB17, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB17_D4_NER
SUB17_D4_NER


title: SUB18, B cells, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB18_D4_BCR
SUB18_D4_BCR


title: SUB17, B cells, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB17_D4_BCR
SUB17_D4_BCR


title: SUB17, T cells, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB17_D36_TCR
SUB17_D36_TCR


title: SUB18, NK cells, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB18_D36_NKR
SUB18_D36_NKR


title: SUB17, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB17_D36_NER
SUB17_D36_NER


title: SUB17, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB17_D36_MNR
SUB17_D36_MNR


title: SUB17, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB17_D36_NKR
SUB17_D36_NKR


title: SUB17, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB17_D36_DNR
SUB17_D36_DNR


title: SUB17, B cells, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB17_D36_BCR
SUB17_D36_BCR


title: SUB17, T cells, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB17_D32_TCR
SUB17_D32_TCR


title: SUB18, NK cells, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB18_D.7_NKR
SUB18_D.7_NKR


title: SUB17, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB17_D32_NKR
SUB17_D32_NKR


title: SUB17, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB17_D32_MNR
SUB17_D32_MNR


title: SUB17, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB17_D32_DNR
SUB17_D32_DNR


title: SUB17, B cells, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB17_D32_BCR
SUB17_D32_BCR


title: SUB17, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB17_D30_NKR
SUB17_D30_NKR


title: SUB17, T cells, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB17_D30_TCR
SUB17_D30_TCR


title: SUB17, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB17_D30_DNR
SUB17_D30_DNR


title: SUB17, T cells, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB17_D2_TCR
SUB17_D2_TCR


title: SUB17, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB17_D2_NKR
SUB17_D2_NKR


title: SUB17, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB17_D30_MNR
SUB17_D30_MNR


title: SUB17, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB17_D2_NER
SUB17_D2_NER


title: SUB17, B cells, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB17_D30_BCR
SUB17_D30_BCR


title: SUB17, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB17_D2_MNR
SUB17_D2_MNR


title: SUB17, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB17_D2_DNR
SUB17_D2_DNR


title: SUB17, B cells, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB17_D2_BCR
SUB17_D2_BCR


title: SUB17, T cells, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB17_D29_TCR
SUB17_D29_TCR


title: SUB17, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB17_D29_NKR
SUB17_D29_NKR


title: SUB17, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB17_D29_NER
SUB17_D29_NER


title: SUB17, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB17_D29_MNR
SUB17_D29_MNR


title: SUB18, T cells, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB18_D32_TCR
SUB18_D32_TCR


title: SUB17, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB17_D29_DNR
SUB17_D29_DNR


title: SUB17, T cells, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB17_D1_TCR
SUB17_D1_TCR


title: SUB17, B cells, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB17_D29_BCR
SUB17_D29_BCR


title: SUB17, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB17_D1_NKR
SUB17_D1_NKR


title: SUB17, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB17_D1_NER
SUB17_D1_NER


title: SUB17, B cells, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB17_D1_BCR
SUB17_D1_BCR


title: SUB17, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB17_D1_DNR
SUB17_D1_DNR


title: SUB17, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB17_D1_MNR
SUB17_D1_MNR


title: SUB17, T cells, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB17_D.7_TCR
SUB17_D.7_TCR


title: SUB17, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB17_D.7_NKR
SUB17_D.7_NKR


title: SUB17, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB17_D.7_NER
SUB17_D.7_NER


title: SUB17, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB17_D.7_MNR
SUB17_D.7_MNR


title: SUB17, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB17_D.7_DNR
SUB17_D.7_DNR


title: SUB16, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB16_D4_NKR
SUB16_D4_NKR


title: SUB16, T cells, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB16_D4_TCR
SUB16_D4_TCR


title: SUB16, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB16_D4_NER
SUB16_D4_NER


title: SUB16, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB16_D4_MNR
SUB16_D4_MNR


title: SUB16, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB16_D4_DNR
SUB16_D4_DNR


title: SUB16, B cells, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB16_D4_BCR
SUB16_D4_BCR


title: SUB16, T cells, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB16_D36_TCR
SUB16_D36_TCR


title: SUB16, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB16_D36_NKR
SUB16_D36_NKR


title: SUB16, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB16_D36_MNR
SUB16_D36_MNR


title: SUB16, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB16_D36_NER
SUB16_D36_NER


title: SUB16, B cells, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB16_D36_BCR
SUB16_D36_BCR


title: SUB16, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB16_D36_DNR
SUB16_D36_DNR


title: SUB16, T cells, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB16_D32_TCR
SUB16_D32_TCR


title: SUB16, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB16_D32_NKR
SUB16_D32_NKR


title: SUB16, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB16_D32_NER
SUB16_D32_NER


title: SUB16, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB16_D32_MNR
SUB16_D32_MNR


title: SUB16, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB16_D32_DNR
SUB16_D32_DNR


title: SUB16, B cells, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB16_D32_BCR
SUB16_D32_BCR


title: SUB16, T cells, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB16_D30_TCR
SUB16_D30_TCR


title: SUB16, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB16_D30_NKR
SUB16_D30_NKR


title: SUB16, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB16_D30_NER
SUB16_D30_NER


title: SUB16, B cells, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB16_D30_BCR
SUB16_D30_BCR


title: SUB16, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB16_D30_MNR
SUB16_D30_MNR


title: SUB16, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB16_D30_DNR
SUB16_D30_DNR


title: SUB16, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB16_D2_NKR
SUB16_D2_NKR


title: SUB16, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB16_D2_NER
SUB16_D2_NER


title: SUB16, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB16_D2_MNR
SUB16_D2_MNR


title: SUB16, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB16_D2_DNR
SUB16_D2_DNR


title: SUB16, B cells, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB16_D2_BCR
SUB16_D2_BCR


title: SUB16, T cells, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB16_D29_TCR
SUB16_D29_TCR


title: SUB16, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB16_D29_NKR
SUB16_D29_NKR


title: SUB16, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB16_D29_NER
SUB16_D29_NER


title: SUB16, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB16_D29_MNR
SUB16_D29_MNR


title: SUB16, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB16_D29_DNR
SUB16_D29_DNR


title: SUB16, T cells, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB16_D1_TCR
SUB16_D1_TCR


title: SUB16, B cells, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB16_D29_BCR
SUB16_D29_BCR


title: SUB16, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB16_D1_NKR
SUB16_D1_NKR


title: SUB16, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB16_D1_NER
SUB16_D1_NER


title: SUB16, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB16_D1_MNR
SUB16_D1_MNR


title: SUB16, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB16_D1_DNR
SUB16_D1_DNR


title: SUB16, B cells, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB16_D1_BCR
SUB16_D1_BCR


title: SUB16, T cells, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB16_D.7_TCR
SUB16_D.7_TCR


title: SUB16, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB16_D.7_NKR
SUB16_D.7_NKR


title: SUB16, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB16_D.7_NER
SUB16_D.7_NER


title: SUB16, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB16_D.7_MNR
SUB16_D.7_MNR


title: SUB16, B cells, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB16_D.7_BCR
SUB16_D.7_BCR


title: SUB16, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB16_D.7_DNR
SUB16_D.7_DNR


title: SUB15, T cells, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB15_D4_TCR
SUB15_D4_TCR


title: SUB15, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB15_D4_NER
SUB15_D4_NER


title: SUB15, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB15_D4_MNR
SUB15_D4_MNR


title: SUB15, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB15_D4_DNR
SUB15_D4_DNR


title: SUB15, B cells, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB15_D4_BCR
SUB15_D4_BCR


title: SUB15, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB15_D4_NKR
SUB15_D4_NKR


title: SUB15, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB15_D36_NKR
SUB15_D36_NKR


title: SUB15, B cells, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB15_D36_BCR
SUB15_D36_BCR


title: SUB15, T cells, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB15_D36_TCR
SUB15_D36_TCR


title: SUB15, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB15_D36_DNR
SUB15_D36_DNR


title: SUB15, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB15_D36_NER
SUB15_D36_NER


title: SUB15, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB15_D36_MNR
SUB15_D36_MNR


title: SUB15, T cells, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB15_D32_TCR
SUB15_D32_TCR


title: SUB15, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB15_D32_NKR
SUB15_D32_NKR


title: SUB15, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB15_D32_NER
SUB15_D32_NER


title: SUB15, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB15_D32_MNR
SUB15_D32_MNR


title: SUB15, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB15_D32_DNR
SUB15_D32_DNR


title: SUB15, B cells, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB15_D32_BCR
SUB15_D32_BCR


title: SUB15, T cells, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB15_D30_TCR
SUB15_D30_TCR


title: SUB15, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB15_D30_NKR
SUB15_D30_NKR


title: SUB15, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB15_D30_NER
SUB15_D30_NER


title: SUB15, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB15_D30_DNR
SUB15_D30_DNR


title: SUB15, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB15_D30_MNR
SUB15_D30_MNR


title: SUB15, B cells, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB15_D30_BCR
SUB15_D30_BCR


title: SUB15, T cells, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB15_D2_TCR
SUB15_D2_TCR


title: SUB15, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB15_D2_NER
SUB15_D2_NER


title: SUB15, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB15_D2_MNR
SUB15_D2_MNR


title: SUB15, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB15_D2_NKR
SUB15_D2_NKR


title: SUB15, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB15_D2_DNR
SUB15_D2_DNR


title: SUB15, B cells, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB15_D2_BCR
SUB15_D2_BCR


title: SUB15, T cells, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB15_D29_TCR
SUB15_D29_TCR


title: SUB15, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB15_D29_NER
SUB15_D29_NER


title: SUB15, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB15_D29_NKR
SUB15_D29_NKR


title: SUB15, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB15_D29_DNR
SUB15_D29_DNR


title: SUB15, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB15_D29_MNR
SUB15_D29_MNR


title: SUB15, B cells, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB15_D29_BCR
SUB15_D29_BCR


title: SUB15, T cells, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB15_D1_TCR
SUB15_D1_TCR


title: SUB15, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB15_D1_NER
SUB15_D1_NER


title: SUB15, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB15_D1_NKR
SUB15_D1_NKR


title: SUB15, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB15_D1_MNR
SUB15_D1_MNR


title: SUB15, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB15_D1_DNR
SUB15_D1_DNR


title: SUB15, T cells, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB15_D.7_TCR
SUB15_D.7_TCR


title: SUB15, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB15_D.7_NKR
SUB15_D.7_NKR


title: SUB15, B cells, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB15_D1_BCR
SUB15_D1_BCR


title: SUB15, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB15_D.7_NER
SUB15_D.7_NER


title: SUB15, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB15_D.7_MNR
SUB15_D.7_MNR


title: SUB15, B cells, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB15_D.7_BCR
SUB15_D.7_BCR


title: SUB15, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB15_D.7_DNR
SUB15_D.7_DNR


title: SUB14, T cells, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB14_D4_TCR
SUB14_D4_TCR


title: SUB14, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB14_D4_NKR
SUB14_D4_NKR


title: SUB14, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB14_D4_NER
SUB14_D4_NER


title: SUB14, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB14_D4_MNR
SUB14_D4_MNR


title: SUB14, B cells, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB14_D4_BCR
SUB14_D4_BCR


title: SUB14, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB14_D4_DNR
SUB14_D4_DNR


title: SUB14, T cells, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB14_D36_TCR
SUB14_D36_TCR


title: SUB14, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB14_D36_NKR
SUB14_D36_NKR


title: SUB14, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB14_D36_NER
SUB14_D36_NER


title: SUB14, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB14_D36_MNR
SUB14_D36_MNR


title: SUB14, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB14_D36_DNR
SUB14_D36_DNR


title: SUB14, B cells, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB14_D36_BCR
SUB14_D36_BCR


title: SUB14, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB14_D32_MNR
SUB14_D32_MNR


title: SUB14, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB14_D32_NKR
SUB14_D32_NKR


title: SUB14, T cells, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB14_D32_TCR
SUB14_D32_TCR


title: SUB14, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB14_D32_DNR
SUB14_D32_DNR


title: SUB14, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB14_D32_NER
SUB14_D32_NER


title: SUB14, B cells, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB14_D32_BCR
SUB14_D32_BCR


title: SUB14, T cells, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB14_D30_TCR
SUB14_D30_TCR


title: SUB14, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB14_D30_NKR
SUB14_D30_NKR


title: SUB14, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB14_D30_MNR
SUB14_D30_MNR


title: SUB14, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB14_D30_NER
SUB14_D30_NER


title: SUB14, T cells, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB14_D2_TCR
SUB14_D2_TCR


title: SUB14, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB14_D30_DNR
SUB14_D30_DNR


title: SUB14, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB14_D2_MNR
SUB14_D2_MNR


title: SUB14, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB14_D2_NER
SUB14_D2_NER


title: SUB14, B cells, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB14_D30_BCR
SUB14_D30_BCR


title: SUB14, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB14_D2_DNR
SUB14_D2_DNR


title: SUB14, T cells, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB14_D29_TCR
SUB14_D29_TCR


title: SUB14, B cells, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB14_D2_BCR
SUB14_D2_BCR


title: SUB14, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB14_D29_NKR
SUB14_D29_NKR


title: SUB14, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB14_D2_NKR
SUB14_D2_NKR


title: SUB14, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB14_D29_NER
SUB14_D29_NER


title: SUB14, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB14_D29_MNR
SUB14_D29_MNR


title: SUB14, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB14_D29_DNR
SUB14_D29_DNR


title: SUB14, B cells, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB14_D29_BCR
SUB14_D29_BCR


title: SUB14, T cells, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB14_D1_TCR
SUB14_D1_TCR


title: SUB14, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB14_D1_NER
SUB14_D1_NER


title: SUB14, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB14_D1_NKR
SUB14_D1_NKR


title: SUB14, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB14_D1_MNR
SUB14_D1_MNR


title: SUB14, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB14_D1_DNR
SUB14_D1_DNR


title: SUB14, B cells, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB14_D1_BCR
SUB14_D1_BCR


title: SUB14, T cells, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB14_D.7_TCR
SUB14_D.7_TCR


title: SUB14, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB14_D.7_NKR
SUB14_D.7_NKR


title: SUB14, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB14_D.7_NER
SUB14_D.7_NER


title: SUB14, B cells, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB14_D.7_BCR
SUB14_D.7_BCR


title: SUB13, T cells, A/H3N2, Day8

description:
Library name: SUB13_D8_TCR
SUB13_D8_TCR


title: SUB13, NK cells, A/H3N2, Day8

description:
Library name: SUB13_D8_NKR
SUB13_D8_NKR


title: SUB14, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB14_D.7_DNR
SUB14_D.7_DNR


title: SUB20, Monocytes, A/H3N2, Day8

description:
Library name: SUB20_D8_MNR
SUB20_D8_MNR


title: SUB13, Neutrophils, A/H3N2, Day8

description:
Library name: SUB13_D8_NER
SUB13_D8_NER


title: SUB13, Monocytes, A/H3N2, Day8

description:
Library name: SUB13_D8_MNR
SUB13_D8_MNR


title: SUB13, B cells, A/H3N2, Day8

description:
Library name: SUB13_D8_BCR
SUB13_D8_BCR


title: SUB13, T cells, A/H3N2, Day4

description:
Library name: SUB13_D4_TCR
SUB13_D4_TCR


title: SUB13, Dendritic cells, A/H3N2, Day8

description:
Library name: SUB13_D8_DNR
SUB13_D8_DNR


title: SUB13, NK cells, A/H3N2, Day4

description:
Library name: SUB13_D4_NKR
SUB13_D4_NKR


title: SUB13, Monocytes, A/H3N2, Day4

description:
Library name: SUB13_D4_MNR
SUB13_D4_MNR


title: SUB13, Neutrophils, A/H3N2, Day4

description:
Library name: SUB13_D4_NER
SUB13_D4_NER


title: SUB13, B cells, A/H3N2, Day4

description:
Library name: SUB13_D4_BCR
SUB13_D4_BCR


title: SUB13, Dendritic cells, A/H3N2, Day4

description:
Library name: SUB13_D4_DNR
SUB13_D4_DNR


title: SUB13, T cells, A/H3N2, Day2

description:
Library name: SUB13_D2_TCR
SUB13_D2_TCR


title: SUB13, NK cells, A/H3N2, Day2

description:
Library name: SUB13_D2_NKR
SUB13_D2_NKR


title: SUB13, Monocytes, A/H3N2, Day2

description:
Library name: SUB13_D2_MNR
SUB13_D2_MNR


title: SUB13, Dendritic cells, A/H3N2, Day2

description:
Library name: SUB13_D2_DNR
SUB13_D2_DNR


title: SUB13, T cells, A/H3N2, Day1

description:
Library name: SUB13_D1_TCR
SUB13_D1_TCR


title: SUB13, NK cells, A/H3N2, Day1

description:
Library name: SUB13_D1_NKR
SUB13_D1_NKR


title: SUB13, Monocytes, A/H3N2, Day1

description:
Library name: SUB13_D1_MNR
SUB13_D1_MNR


title: SUB13, Neutrophils, A/H3N2, Day1

description:
Library name: SUB13_D1_NER
SUB13_D1_NER


title: SUB14, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB14_D.7_MNR
SUB14_D.7_MNR


title: SUB13, Dendritic cells, A/H3N2, Day1

description:
Library name: SUB13_D1_DNR
SUB13_D1_DNR


title: SUB13, T cells, A/H3N2, Day-7

description:
Library name: SUB13_D.7_TCR
SUB13_D.7_TCR


title: SUB13, NK cells, A/H3N2, Day-7

description:
Library name: SUB13_D.7_NKR
SUB13_D.7_NKR


title: SUB13, B cells, A/H3N2, Day1

description:
Library name: SUB13_D1_BCR
SUB13_D1_BCR


title: SUB13, Monocytes, A/H3N2, Day-7

description:
Library name: SUB13_D.7_MNR
SUB13_D.7_MNR


title: SUB12, Neutrophils, A/H3N2, Day8

description:
Library name: SUB12_D8_NER
SUB12_D8_NER


title: SUB12, T cells, A/H3N2, Day8

description:
Library name: SUB12_D8_TCR
SUB12_D8_TCR


title: SUB13, Neutrophils, A/H3N2, Day-7

description:
Library name: SUB13_D.7_NER
SUB13_D.7_NER


title: SUB12, Monocytes, A/H3N2, Day8

description:
Library name: SUB12_D8_MNR
SUB12_D8_MNR


title: SUB12, Dendritic cells, A/H3N2, Day8

description:
Library name: SUB12_D8_DNR
SUB12_D8_DNR


title: SUB12, B cells, A/H3N2, Day8

description:
Library name: SUB12_D8_BCR
SUB12_D8_BCR


title: SUB12, T cells, A/H3N2, Day4

description:
Library name: SUB12_D4_TCR
SUB12_D4_TCR


title: SUB12, NK cells, A/H3N2, Day4

description:
Library name: SUB12_D4_NKR
SUB12_D4_NKR


title: SUB12, Neutrophils, A/H3N2, Day4

description:
Library name: SUB12_D4_NER
SUB12_D4_NER


title: SUB12, Dendritic cells, A/H3N2, Day4

description:
Library name: SUB12_D4_DNR
SUB12_D4_DNR


title: SUB12, Monocytes, A/H3N2, Day4

description:
Library name: SUB12_D4_MNR
SUB12_D4_MNR


title: SUB12, B cells, A/H3N2, Day4

description:
Library name: SUB12_D4_BCR
SUB12_D4_BCR


title: SUB12, NK cells, A/H3N2, Day2

description:
Library name: SUB12_D2_NKR
SUB12_D2_NKR


title: SUB12, T cells, A/H3N2, Day2

description:
Library name: SUB12_D2_TCR
SUB12_D2_TCR


title: SUB12, Neutrophils, A/H3N2, Day2

description:
Library name: SUB12_D2_NER
SUB12_D2_NER


title: SUB12, Monocytes, A/H3N2, Day2

description:
Library name: SUB12_D2_MNR
SUB12_D2_MNR


title: SUB12, Dendritic cells, A/H3N2, Day2

description:
Library name: SUB12_D2_DNR
SUB12_D2_DNR


title: SUB12, B cells, A/H3N2, Day2

description:
Library name: SUB12_D2_BCR
SUB12_D2_BCR


title: SUB12, T cells, A/H3N2, Day1

description:
Library name: SUB12_D1_TCR
SUB12_D1_TCR


title: SUB12, NK cells, A/H3N2, Day8

description:
Library name: SUB12_D8_NKR
SUB12_D8_NKR


title: SUB12, NK cells, A/H3N2, Day1

description:
Library name: SUB12_D1_NKR
SUB12_D1_NKR


title: SUB12, Monocytes, A/H3N2, Day1

description:
Library name: SUB12_D1_MNR
SUB12_D1_MNR


title: SUB12, Dendritic cells, A/H3N2, Day1

description:
Library name: SUB12_D1_DNR
SUB12_D1_DNR


title: SUB12, T cells, A/H3N2, Day-7

description:
Library name: SUB12_D.7_TCR
SUB12_D.7_TCR


title: SUB12, Dendritic cells, A/H3N2, Day-7

description:
Library name: SUB12_D.7_DNR
SUB12_D.7_DNR


title: SUB12, NK cells, A/H3N2, Day-7

description:
Library name: SUB12_D.7_NKR
SUB12_D.7_NKR


title: SUB12, Monocytes, A/H3N2, Day-7

description:
Library name: SUB12_D.7_MNR
SUB12_D.7_MNR


title: SUB12, B cells, A/H3N2, Day-7

description:
Library name: SUB12_D.7_BCR
SUB12_D.7_BCR


title: SUB11, T cells, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB11_D4_TCR
SUB11_D4_TCR


title: SUB12, Neutrophils, A/H3N2, Day-7

description:
Library name: SUB12_D.7_NER
SUB12_D.7_NER


title: SUB11, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB11_D4_NER
SUB11_D4_NER


title: SUB11, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB11_D4_NKR
SUB11_D4_NKR


title: SUB13, Dendritic cells, A/H3N2, Day-7

description:
Library name: SUB13_D.7_DNR
SUB13_D.7_DNR


title: SUB13, B cells, A/H3N2, Day-7

description:
Library name: SUB13_D.7_BCR
SUB13_D.7_BCR


title: SUB11, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB11_D4_MNR
SUB11_D4_MNR


title: SUB11, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB11_D4_DNR
SUB11_D4_DNR


title: SUB11, T cells, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB11_D36_TCR
SUB11_D36_TCR


title: SUB11, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB11_D36_NKR
SUB11_D36_NKR


title: SUB11, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB11_D36_MNR
SUB11_D36_MNR


title: SUB11, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB11_D36_DNR
SUB11_D36_DNR


title: SUB11, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB11_D36_NER
SUB11_D36_NER


title: SUB11, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB11_D32_NKR
SUB11_D32_NKR


title: SUB11, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB11_D32_MNR
SUB11_D32_MNR


title: SUB11, T cells, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB11_D32_TCR
SUB11_D32_TCR


title: SUB11, B cells, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB11_D32_BCR
SUB11_D32_BCR


title: SUB11, B cells, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB11_D36_BCR
SUB11_D36_BCR


title: SUB11, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB11_D30_NKR
SUB11_D30_NKR


title: SUB11, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB11_D32_DNR
SUB11_D32_DNR


title: SUB11, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB11_D30_NER
SUB11_D30_NER


title: SUB11, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB11_D30_MNR
SUB11_D30_MNR


title: SUB11, B cells, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB11_D30_BCR
SUB11_D30_BCR


title: SUB11, T cells, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB11_D2_TCR
SUB11_D2_TCR


title: SUB11, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB11_D30_DNR
SUB11_D30_DNR


title: SUB11, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB11_D2_NKR
SUB11_D2_NKR


title: SUB11, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB11_D2_NER
SUB11_D2_NER


title: SUB11, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB11_D2_MNR
SUB11_D2_MNR


title: SUB11, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB11_D2_DNR
SUB11_D2_DNR


title: SUB11, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB11_D29_NKR
SUB11_D29_NKR


title: SUB11, B cells, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB11_D2_BCR
SUB11_D2_BCR


title: SUB11, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB11_D29_MNR
SUB11_D29_MNR


title: SUB11, B cells, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB11_D29_BCR
SUB11_D29_BCR


title: SUB11, T cells, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB11_D29_TCR
SUB11_D29_TCR


title: SUB11, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB11_D29_DNR
SUB11_D29_DNR


title: SUB11, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB11_D29_NER
SUB11_D29_NER


title: SUB11, T cells, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB11_D1_TCR
SUB11_D1_TCR


title: SUB11, T cells, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB11_D30_TCR
SUB11_D30_TCR


title: SUB11, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB11_D1_NKR
SUB11_D1_NKR


title: SUB11, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB11_D1_MNR
SUB11_D1_MNR


title: SUB11, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB11_D1_NER
SUB11_D1_NER


title: SUB11, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB11_D1_DNR
SUB11_D1_DNR


title: SUB11, B cells, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB11_D1_BCR
SUB11_D1_BCR


title: SUB11, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB11_D.7_NKR
SUB11_D.7_NKR


title: SUB11, T cells, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB11_D.7_TCR
SUB11_D.7_TCR


title: SUB11, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB11_D.7_NER
SUB11_D.7_NER


title: SUB11, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB11_D.7_MNR
SUB11_D.7_MNR


title: SUB10, NK cells, A/H3N2, Day8

description:
Library name: SUB10_D8_NKR
SUB10_D8_NKR


title: SUB10, T cells, A/H3N2, Day8

description:
Library name: SUB10_D8_TCR
SUB10_D8_TCR


title: SUB11, B cells, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB11_D.7_BCR
SUB11_D.7_BCR


title: SUB11, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB11_D.7_DNR
SUB11_D.7_DNR


title: SUB10, Monocytes, A/H3N2, Day8

description:
Library name: SUB10_D8_MNR
SUB10_D8_MNR


title: SUB10, Dendritic cells, A/H3N2, Day8

description:
Library name: SUB10_D8_DNR
SUB10_D8_DNR


title: SUB10, Neutrophils, A/H3N2, Day8

description:
Library name: SUB10_D8_NER
SUB10_D8_NER


title: SUB10, B cells, A/H3N2, Day8

description:
Library name: SUB10_D8_BCR
SUB10_D8_BCR


title: Treg (CD127-CD25+) - Donor 123 - Day 1

city: Seattle

first: Laura

last: Cooeny

state: WA

address: 1201 9th Ave

department: University of Michigan

institute: Immune Tolerance Network

description:
Library name: lib59036_AAAFM27HV
Donor ID: 123; Study day: D1

data_processing:
Adapter and quality trimming of raw reads was performed using FastqMcf (ea-utils).
Read quality was assessed using FastQC (v0.11.3).
Alignment metrics, duplication rates, and RNA-seq QC metrics (ribosomal/intronic/UTR/coding base composition, 5'/3' bias) were computed using Picard tools (v1.134; CollectRnaSeqMetrics, MarkDuplicates) and Samtools (v1.2).
Base-calling and demultiplexing were performed automatically on BaseSpace (Illumina) to generate FASTQ files.
Reads were aligned to the reference genome using STAR (v2.4.2a); gene-level read counts were generated using HTSeq-count (v0.4.1) in 'intersection (nonempty)' mode with a minimum alignment quality of 20.
Assembly: GRCh38, Ensembl release 91
Supplementary files format and content: Comma-separated file (combined_counts_GEO_submission.csv) containing gene-level raw read counts, with Ensembl gene IDs as rows and library names (matching "library name" column in SAMPLES section) as columns.

source_name: PBMC

extract_protocol:
PBMCs were thawed and immediately immunolabeled and FACS-sorted for sequencing. Dead cells were excluded from all sorts by staining with the Fixable Viability Dye eFluor™ 780 (eBioscience). For bulk RNA-seq, 250–500 CD4+CD127loCD25+ Tregs were sorted directly into lysis buffer, and cDNA was prepared using the SMART-Seq Ultra Low Input RNA Kit for Sequencing (Takara Bio).
RNA-seq libraries were constructed using the NexteraXT DNA Library Preparation Kit (Illumina) with half the recommended volumes and reagents. Paired-end sequencing of pooled libraries was run on a NextSeq 2000 (Illumina) with 59-base reads and a target depth of 5 million reads per sample.

treatment_protocol: Healthy adult volunteers in the Phase 1a single ascending dose trial received a single dose of Placebo, 1mg, 3.5mg, 5mg, or 10mg IL-2 mutein MK-6194/PT-101 subcutaneously. PBMC samples were collected at pre-treatment baseline (-10) and on study days 1 (prior to the dose, which occurred on day 1), 8, 15, and 29 and cryopreserved. For details on the clinical trial, see Scheid, et al., Safety, pharmacokinetics, and pharmacodynamics of MK-6194, an IL-2 mutein designed to selectively activate regulatory T cells: single ascending dose and multiple ascending dose trial data, ImmunoHorizons, Volume 9, Issue 5, May 2025, vlaf005, https://doi.org/10.1093/immhor/vlaf005.

value: PBMC

value: 10mg mutein

tag: batch


title: SUB10, T cells, A/H3N2, Day4

description:
Library name: SUB10_D4_TCR
SUB10_D4_TCR

tag: treatment


title: SUB10, NK cells, A/H3N2, Day4

description:
Library name: SUB10_D4_NKR
SUB10_D4_NKR


title: DMSO SPEN WT RNA 1

first: Sonali

last: Arora

address: 1100 Fairview Ave N,

institute: FHCRC

description:
Library name: DMSO SPEN WT RNA 1
DMSO SPEN WT RNA 1
SB7_S7

data_processing:
Amplified, target-enriched libraries were pooled equimolarly and sequenced on an Illumina NovaSeq 6000 platform (Illumina, San Diego, CA, USA), employing a 2x 100-cycle paired-end sequencing strategy
FASTQ files were generated using Illumina’s bclconvert v3.10.5 and trimmed with Agilent Locatit v3.0
The  sequencing reads were aligned to the Gencode GRCh38.primary_assembly reference genome using STAR29 (v2.7.7a).
Gene-level quantification was conducted with HTSeq30 (v0.11.0) using Gencode V22 primary assembly annotations.
Counts per million ( cpm) reads were calculated using the cpm() function from R package, edgeR
Assembly: hg38
Supplementary files format and content: tab-delimited text files include counts per million (cpm) values for each Sample.

source_name: total RNA

growth_protocol: Total RNA and poylsome associated RNA was extracted from prostate cancer cell line samples using the Maxwell RSC RNA FFPE Kit (Promega, Madison, WI, USA) on the automated Maxwell RSC 48 instrument (Promega)

extract_protocol:
RNA-seq libraries were prepared from total RNA using the TruSeq Stranded mRNA kit (Illumina, Inc., San Diego, CA, USA). Library size distribution was validated using an Agilent 4200 TapeStation (Agilent Technologies, Santa Clara, CA, USA).  Additional library QC, blending of pooled indexed libraries, and cluster optimization was performed using Life Technologies’ Invitrogen Qubit® 2.0 Fluorometer (Life Technologies-Invitrogen, Carlsbad, CA, USA).
RNA-seq libraries were pooled (70-plex) and clustered onto an P2-100 flow cell.

treatment_protocol: untreated

value: total RNA

tag: genotype

value: WT

value: DMSO


title: RNA seq, KO, male mice-3, ~20w

city: New Haven

first: Li

last: Wen

address: 300 Cedar Street

institute: Yale Univeristy

description: Library name: 8NFcre

data_processing:
Raw fastq files were processed using fastq tool.
Sequencing reads with low-quality bases were trimmed and filtered
Cleaned reads were aligned using STAR (version 2.7.9) with the mouse reference genome gencode version GRCm38.p6 with vM25 gene annotation.
Expression quantification for aligned reads was performed using featureCounts (version 2.0.0)
The filtered read counts matrix was normalized by the transcripts per million (TPM) method.
Assembly: mm10
Supplementary files format and content: csv files

organism: Mus musculus

source_name: islet

extract_protocol:
RNA was harvested using RNA mini kit (Qiangen)
RNA library for RNA-seq were prepared using KARA mRNA HyperPrep kit following manufacturer's instructions.

value: islet

tag: cell type

value: beta cell

value: Pdgfrafl/fl Pdx1-Cre+ C57BL/6

tag: background strain

value: ko

tag: Sex

value: male


title: SUB10, Neutrophils, A/H3N2, Day4

description:
Library name: SUB10_D4_NER
SUB10_D4_NER

tag: day


title: Enzalutamide SPEN KO RNA 3

description:
Library name: Enzalutamide SPEN KO RNA 3
Enzalutamide SPEN KO RNA 3
SB6_S6

value: KO

value: Enzalutamide


title: intestinal epithelial cells, 25-week-old, wild type

city: Hangzhou

first: Guo-shi

last: Chai

state: --- Select One ---

address: No. 600 Dunyu Road, Sandun Town, Xihu District, Hangzhou, Zhejiang PR China

institute: Westlake University

description:
Library name: sample1
10x Genomics

data_processing:
The demultiplexing, barcoded processing, gene counting and aggregation were made using the Cellanger software 7.0.0
Assembly: mm10
Supplementary files format and content: Tab-separated values files and matrix files

source_name: small intestine

extract_protocol:
Mouse small intestines were excised and flushed with cold PBS. The tissues were opened longitudinally along the lumen and cut into 1 cm segments. The segments were incubated in cold PBS containing 20 mM EDTA for 1 hour on ice. Following incubation, the supernatant was discarded and replaced with fresh cold PBS. The tubes were then vortexed for 30 seconds to dissociate villi and crypts from the basement membrane. The released villi and crypts were transferred to a new tube and digested with TrypLE (Thermo Fisher Scientific, 12604013) supplemented with 10 mg/mL DNase I (Roche, 10104159001) for 10 minutes at 32°C. The digested cell suspension was sequentially filtered through 70-μm and 40-μm cell strainers. Isolated single IECs were centrifuged and resuspended in cold Dulbecco's Phosphate-Buffered Saline (DPBS) containing 2% fetal bovine serum (FBS). For flow cytometry sorting, cell suspensions were stained on ice for 30 minutes with the following antibodies diluted in cold 2% FBS in DPBS: CD45-APC (1:100, clone 30-F11, BioLegend, 103112), CD31-PE (1:50, clone MEC13.3, BioLegend, 102507), TER-119-Brilliant Violet 510™ (1:50, clone TER-119, BioLegend, 116237), and CD326-APC/Cyanine7 (1:100, clone G8.8, BioLegend, 118217). Following incubation, cells were washed with cold 2% FBS in DPBS, resuspended in the same buffer supplemented with 0.1 μg/mL DAPI (BioLegend, 422801), and filtered through a 40-μm cell strainer. Fluorescence-activated cell sorting was performed using a BD FACSAria™ Fusion instrument, and the sorting strategy was visualized using FlowJo Software.
The scRNA-seq was performed using the 10× Chromium Next GEM Automated Single Cell 3′ Reagent Kits v3.1 (1000141) following the manufacturer’s protocol (CG000286 • Rev F). Sorted cells were loaded onto a Chromium Next GEM Automated Chip G (1000136), and single-cell gel bead-in-emulsions (GEMs) were generated using the Chromium Controller Single-Cell Instrument. Following reverse transcription and library construction, library quality was assessed using the Qubit dsDNA HS Assay Kit (Invitrogen) and the High Sensitivity DNA chip (Agilent). Sequencing was performed on the NovaSeq X Plus platform using a 2 × 150 bp paired-end read configuration.

value: small intestine

tag: cell line

value: epithelial cells

value: intestinal epithelial cells

value: wild type mouse


title: SUB10, Monocytes, A/H3N2, Day4

description:
Library name: SUB10_D4_MNR
SUB10_D4_MNR

value: Monocytes


title: SUB10, Dendritic cells, A/H3N2, Day4

description:
Library name: SUB10_D4_DNR
SUB10_D4_DNR

value: Dendritic cells


title: SUB10, B cells, A/H3N2, Day4

description:
Library name: SUB10_D4_BCR
SUB10_D4_BCR

value: B cells


title: SUB10, T cells, A/H3N2, Day2

description:
Library name: SUB10_D2_TCR
SUB10_D2_TCR

value: T cells


title: SUB10, NK cells, A/H3N2, Day2

description:
Library name: SUB10_D2_NKR
SUB10_D2_NKR

value: NK cells


title: SUB10, Neutrophils, A/H3N2, Day2

description:
Library name: SUB10_D2_NER
SUB10_D2_NER

value: Neutrophils


title: SUB10, Monocytes, A/H3N2, Day2

description:
Library name: SUB10_D2_MNR
SUB10_D2_MNR


title: SUB10, Dendritic cells, A/H3N2, Day2

description:
Library name: SUB10_D2_DNR
SUB10_D2_DNR