title: N206_SR4835_DMSO_rep1

description:
Library name: RNA019264
N206_SR4835_DMSO_rep1


title: SH-SY5Y_SR4835_rep1

status: Public on Jul 29 2026

type: SRA

city: Ghent

first: Sarah-Lee

last: Bekaert

state: No state

address: Corneel Heymanslaan 10

country: Belgium

institute: UGent

description:
Library name: RNA019263
SH_SY5Y_SR4835_rep1

library_source: transcriptomic

data_processing:
Reads were quality checked with FastQC (v0.11.7) and aligned to the Homo sapiens reference genome (GRCh38.96) using STAR (v2.7.2b).
Post-alignment, read counts were generated and normalized with edgeR (v3.36.0).
After performing PCA plotting, certain replicates were removed from the analysis as they did not cluster with the other replicates, indicating potential outliers or inconsistencies.
Differential expression analysis was performed using the Limma-Voom pipeline (v3.50.3) with three or four replicates per condition.
Assembly: Homo sapiens GRCh38.96
Supplementary files format and content: Tab-delimited text file include STAR counts per experiment

molecule: polyA RNA

organism: Homo sapiens

source_name: bone marrow

extract_protocol:
RNA was isolated as described in the ‘RNA isolation and RT-qPCR’ section. RNA quality was measured using the Fragment analyzer 12 capillary array prior to profiling
A total of 55ng of RNA was used as input for the library preparation with the Illumina Stranded mRNA prep (#20040534). During the library preparation 15 PCR cycles were used. qPCR quantification of the libraries was done using the Kapa Library Quantification Kit for the Illumina LightCycler 480 (Roche diagnostics #07960298001).
RNA-Seq libraries were sequenced on the Illumina NextSeq 2000 platform using the NextSeq 2000 P2 Reagents 100 Cycles single-end kit (Illumina #20100987).

tag: tissue

value: bone marrow

tag: cell line

tag: cell type

value: neuroblast

tag: genotype

value: WT

tag: treatment


title: SUB30, T cells, A/H3N2, Day4

city: Rockville

first: Johannes

last: Goll

state: MD

address: 401 N Washington St

department: Bioinformatics Service Group

country: USA

institute: The Emmes Company

description:
Library name: SUB30_D4_TCR
SUB30_D4_TCR

data_processing:
Human reference genome assembly (GRCh38), gene models, and associated gene annotation information were obtained from the ENSEMBL database (Version 99). The genomic reference was built by merging all human chromosomes except for X and Y chromosomes (to avoid gender-specific effects). Adapter sequences and low-quality 5′ ending sequences were removed from raw sequencing reads using the Trimmomatic software (Version 0.39). Following adapter removal, sequence reads were aligned to the reference transcriptome/genome using HISAT2 splice-aware sequence aligner (Version 2.2.0).  For each sample, the quality of reference alignments was evaluated using the RSeQC software (Version 3.0.1). Gene expression quantification was conducted on the gene level using Subread v2.0.0 counting mapped paired-reads to obtain fragment counts per gene.
Assembly: GRCh38 ENSEMBL database (Version 99)
Supplementary files format and content: tab-delimited text includes raw counts of each ENSEMBL Gene ID for each Sample

molecule: total RNA

source_name: T cells

extract_protocol:
Peripheral blood mononuclear cells (PBMC) were recovered from phlebotomy samples and processed immediately. Magnetic sorting (AutoMACS Pro) was used to isolate individual immune cells using microbeads coated with CD15 (PMN), CD19 (B-cells), CD3 (T-cells), CD56 (NK cells), CD11c (dendritic cells), and CD14 (monocytes). During optimization, flow cytometric analysis confirmed >99% purity of each immune cell compartment. RNA was extracted from PBMC using the Promega simplyRNA Cell Kit according to the manufacturer’s protocol.
Polyadenylated RNAs were captured using NEBNext Magnetic Oligo d(T)25 Beads, and the NEBNext mRNA Library Prep Reagent Set for Illumina (New England BioLabs Inc., Ipswich, MA, USA) was used to prepare individually bar-coded libraries as per manufacturer’s recommended protocol. Accurate quantification for sequencing applications was determined using the qPCR-based KAPA Biosystems Library Quantification Kit (Kapa Biosystems, Inc., Woburn, MA, USA).
Paired-end sequencing (25 million, 75-bp, paired-end reads) was performed on an Illumina Novaseq 6000 sequencers (Illumina, Inc., San Diego, CA, USA) as per manufacturer’s recommended protocol.

value: T cells

tag: day


title: SUB30, NK cells, A/H3N2, Day4

description:
Library name: SUB30_D4_NKR
SUB30_D4_NKR

source_name: NK cells

value: NK cells


title: SUB30, Monocytes, A/H3N2, Day4

description:
Library name: SUB30_D4_MNR
SUB30_D4_MNR

source_name: Monocytes

value: Monocytes


title: SUB30, Dendritic cells, A/H3N2, Day4

description:
Library name: SUB30_D4_DNR
SUB30_D4_DNR

source_name: Dendritic cells

value: Dendritic cells


title: SUB30, T cells, A/H3N2, Day2

description:
Library name: SUB30_D2_TCR
SUB30_D2_TCR


title: SUB30, NK cells, A/H3N2, Day2

description:
Library name: SUB30_D2_NKR
SUB30_D2_NKR


title: SH-SY5Y_SR4835_DMSO_rep1

description:
Library name: RNA019262
SH-SY5Y_SR4835_DMSO_rep1

value: DMSO in SR-4835 experiment


title: SUB30, Neutrophils, A/H3N2, Day4

description:
Library name: SUB30_D4_NER
SUB30_D4_NER

source_name: Neutrophils

value: Neutrophils


title: SUB30, Monocytes, A/H3N2, Day2

description:
Library name: SUB30_D2_MNR
SUB30_D2_MNR


title: SUB30, Dendritic cells, A/H3N2, Day2

description:
Library name: SUB30_D2_DNR
SUB30_D2_DNR


title: SUB30, B cells, A/H3N2, Day2

description:
Library name: SUB30_D2_BCR
SUB30_D2_BCR

source_name: B cells

value: B cells


title: SUB30, B cells, A/H3N2, Day4

description:
Library name: SUB30_D4_BCR
SUB30_D4_BCR


title: SUB30, T cells, A/H3N2, Day1

description:
Library name: SUB30_D1_TCR
SUB30_D1_TCR


title: RNA-seq_sgHBG1_2-2

first: Kailong

last: Li

country: China

institute: Peking University

description: Library name: sgHBG1_2-2

data_processing:
Sequencing reads were first processed using cutadapt (vX.X) with the following parameters: adapter trimming with explicit Illumina adapter sequences provided for both forward (-a AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC) and reverse (-A AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATT) reads, removal of reads containing more than 8 ambiguous bases (--max-n 8), minimum read length filter of 15 bp (--minimum-length 15), and paired-end consistency filtering (--pair-filter=any). Cleaned paired reads were written to output files with 50 parallel threads enabled. Subsequently, adapter-removed reads were subjected to an additional round of quality filtering using Trimmomatic (v0.39) in paired-end mode with the following settings: average read quality threshold of 20 (AVGQUAL:20), sliding window trimming with a window size of 4 and minimum average quality of 15 (SLIDINGWINDOW:4:15), and minimum read length requirement of 15 bp (MINLEN:15). This two-step filtering strategy ensured stringent removal of adapter contamination, low-quality bases, and excessively short or low-confidence reads prior to downstream analyses.
Processed reads were then aligned to human genome assembly hg19 using hisat2  in local alignment mode with default parameters.
Alignment files were processed using samtools (v1.15.1) for sorting and indexing.
The bigWig files were generated using deepTools bamCoverage (v3.5.1) with the following arguments: bin size of 1 bp.
Assembly: hg19
Supplementary files format and content: bigwig

extract_protocol:
Cells were fiex with 1% formaldehyde for 10min\ at RT with shaking.After quenching and cell lysis chromatin was sonicated.
For RNA-seq analysis using the Illumina NovaSeq platform, 500 ng–1 μg of total RNA was processed for library construction

value: Human epithelial-like cell from the kidney


title: SUB30, NK cells, A/H3N2, Day1

description:
Library name: SUB30_D1_NKR
SUB30_D1_NKR


title: SUB30, Monocytes, A/H3N2, Day1

description:
Library name: SUB30_D1_MNR
SUB30_D1_MNR


title: SUB30, Neutrophils, A/H3N2, Day1

description:
Library name: SUB30_D1_NER
SUB30_D1_NER


title: scRNA-seq, P29, before RTX treatment

city: Nagoya City

first: Yu

last: Watanabe

state: Aichi Pref

address: 65, Tsurumai Town, Showa Ward

department: Molecular Oncology

country: Japan

institute: Nagoya University

description: Library name: P29_Pre_GEX

library_source: transcriptomic single cell

data_processing:
Droplet-based sequencing data were aligned and quantified using Cell Ranger 8.0.0(10x Genomics).
Assembly: Homo Sapiens, GRCh38
Supplementary files format and content: Tab-separated values files and matrix files

source_name: PBMC

extract_protocol:
Frozen PBMC samples were thawed rapidly in trypsin-stopping solution (D-MEM Low Glucose, 10% FBS, and 1% penicillin/streptomycin) at 37°C, centrifuged at 400g for 10 minutes at 4°C and the supernatant was discarded. To release cell aggregation, 25 μL of DNase Booster Buffer and 10 μL of DNase I stock solution (#79254, QIAGEN) were added to the pellet and incubated at room temperature for 15 minutes. The samples were washed with trypsin-stopping solution and diluted with PBS. To isolate CD3+ T cells, PBMCs collected at both pre and post were stained and sorted at 4°C using pooled monoclonal antibodies and sorted using FACSAria Fusion flow cytometer (BD Biosciences).
Single-cell 5’ and TCR libraries were prepared following the protocol provided by the 10x Genomics Chromium Next GEM Single Cell 5’ Reagent Kits v2 (Dual Index).

value: PBMC

value: CD3+ T-cells

value: before RTX treatment


title: SUB30, Dendritic cells, A/H3N2, Day1

description:
Library name: SUB30_D1_DNR
SUB30_D1_DNR


title: SUB30, B cells, A/H3N2, Day1

description:
Library name: SUB30_D1_BCR
SUB30_D1_BCR


title: TCR-seq, P18, before RTX treatment

description: Library name: P18_Pre_TCR

data_processing:
Droplet-based sequencing data were aligned and quantified using Cell Ranger 8.0.0(10x Genomics).
Assembly: Homo Sapiens, GRCh38
Supplementary files format and content: Tab-separated values files and matrix files
Library strategy: TCR-Seq


title: TCR-seq, P15, after RTX treatment

description: Library name: P15_Post_TCR

value: after RTX treatment


title: TCR-seq, P18, after RTX treatment

description: Library name: P18_Post_TCR


title: scRNA-seq, P15, after RTX treatment

description: Library name: P15_Post_GEX


title: SUB30, T cells, A/H3N2, Day-7

description:
Library name: SUB30_D.7_TCR
SUB30_D.7_TCR


title: SUB30, Neutrophils, A/H3N2, Day2

description:
Library name: SUB30_D2_NER
SUB30_D2_NER


title: scRNA-seq, P04, after RTX treatment

description: Library name: P04_Post_GEX


title: SUB30, NK cells, A/H3N2, Day-7

description:
Library name: SUB30_D.7_NKR
SUB30_D.7_NKR


title: scRNA-seq, P01, after RTX treatment

description: Library name: P01_Post_GEX


title: SUB30, Neutrophils, A/H3N2, Day-7

description:
Library name: SUB30_D.7_NER
SUB30_D.7_NER


title: SUB30, Monocytes, A/H3N2, Day-7

description:
Library name: SUB30_D.7_MNR
SUB30_D.7_MNR


title: SUB30, Dendritic cells, A/H3N2, Day-7

description:
Library name: SUB30_D.7_DNR
SUB30_D.7_DNR


title: SUB30, B cells, A/H3N2, Day-7

description:
Library name: SUB30_D.7_BCR
SUB30_D.7_BCR


title: SUB29, T cells, A/H3N2, Day8

description:
Library name: SUB29_D8_TCR
SUB29_D8_TCR


title: SUB29, NK cells, A/H3N2, Day8

description:
Library name: SUB29_D8_NKR
SUB29_D8_NKR


title: SUB29, Neutrophils, A/H3N2, Day8

description:
Library name: SUB29_D8_NER
SUB29_D8_NER


title: MV4-11_sh-ENO1-2

city: Suzhou

first: Fang

last: Fang

state: Jiangsu

address: 92 Zhongnan street

institute: Children’s Hospital of Soochow University

description:
Library name: Sample 5
MV4-11 sample with ENO1 knockdown

data_processing:
Illumina Casava2.19 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to hg38 whole genome using hisat2 v2.0.5.
Assembly: hg38
Supplementary files format and content: tab-delimited text files include counts for each Sample

extract_protocol:
RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols

value: AML cell line

value: ENO1 knockdown


title: SUB29, Dendritic cells, A/H3N2, Day8

description:
Library name: SUB29_D8_DNR
SUB29_D8_DNR


title: SUB29, Monocytes, A/H3N2, Day8

description:
Library name: SUB29_D8_MNR
SUB29_D8_MNR


title: SUB29, B cells, A/H3N2, Day8

description:
Library name: SUB29_D8_BCR
SUB29_D8_BCR


title: SUB29, T cells, A/H3N2, Day4

description:
Library name: SUB29_D4_TCR
SUB29_D4_TCR


title: SUB29, NK cells, A/H3N2, Day4

description:
Library name: SUB29_D4_NKR
SUB29_D4_NKR


title: SUB29, Neutrophils, A/H3N2, Day4

description:
Library name: SUB29_D4_NER
SUB29_D4_NER


title: SUB29, Monocytes, A/H3N2, Day4

description:
Library name: SUB29_D4_MNR
SUB29_D4_MNR


title: MV4-11_NC-1

description:
Library name: Sample 1
MV4-11 sample with no treatment


title: SUB29, NK cells, A/H3N2, Day2

description:
Library name: SUB29_D2_NKR
SUB29_D2_NKR


title: SUB29, B cells, A/H3N2, Day4

description:
Library name: SUB29_D4_BCR
SUB29_D4_BCR


title: SUB29, T cells, A/H3N2, Day2

description:
Library name: SUB29_D2_TCR
SUB29_D2_TCR


title: SUB29, Dendritic cells, A/H3N2, Day4

description:
Library name: SUB29_D4_DNR
SUB29_D4_DNR


title: SUB29, Monocytes, A/H3N2, Day2

description:
Library name: SUB29_D2_MNR
SUB29_D2_MNR


title: SUB29, Dendritic cells, A/H3N2, Day2

description:
Library name: SUB29_D2_DNR
SUB29_D2_DNR


title: SUB29, Neutrophils, A/H3N2, Day2

description:
Library name: SUB29_D2_NER
SUB29_D2_NER


title: SUB29, B cells, A/H3N2, Day2

description:
Library name: SUB29_D2_BCR
SUB29_D2_BCR


title: SUB29, T cells, A/H3N2, Day1

description:
Library name: SUB29_D1_TCR
SUB29_D1_TCR


title: SY5Y_dCeMM2_IC50_rep2

description:
Library name: RNA019348
all_raw_counts_dCeMM2_Drugging.txt

extract_protocol:
RNA was isolated as described in the ‘RNA isolation and RT-qPCR’ section. RNA quality was measured using the Fragment analyzer 12 capillary array prior to profiling
A total of 55ng of RNA was used as input for the library preparation with the Illumina Stranded mRNA prep (#20040534). During the library preparation 15 PCR cycles were used. qPCR quantification of the libraries was done using the Kapa Library Quantification Kit for the Illumina LightCycler 480 (Roche diagnostics #07960298001).

value: bone marrow

value: GI50 dCeMM2


title: N206_dCeMM2_IC50_rep2

description:
Library name: RNA019350
all_raw_counts_dCeMM2_Drugging.txt

source_name: neuroblastoma cell line

value: neuroblastoma cell line

value: neuroblast

value: WT


title: SUB29, NK cells, A/H3N2, Day1

description:
Library name: SUB29_D1_NKR
SUB29_D1_NKR

value: NK cells


title: SUB29, Monocytes, A/H3N2, Day1

description:
Library name: SUB29_D1_MNR
SUB29_D1_MNR

value: Monocytes


title: SUB29, Neutrophils, A/H3N2, Day1

description:
Library name: SUB29_D1_NER
SUB29_D1_NER


title: SUB29, Dendritic cells, A/H3N2, Day1

description:
Library name: SUB29_D1_DNR
SUB29_D1_DNR

value: Dendritic cells


title: SUB29, T cells, A/H3N2, Day-7

description:
Library name: SUB29_D.7_TCR
SUB29_D.7_TCR

value: T cells


title: SUB29, NK cells, A/H3N2, Day-7

description:
Library name: SUB29_D.7_NKR
SUB29_D.7_NKR


title: SUB29, Monocytes, A/H3N2, Day-7

description:
Library name: SUB29_D.7_MNR
SUB29_D.7_MNR


title: SUB29, Neutrophils, A/H3N2, Day-7

description:
Library name: SUB29_D.7_NER
SUB29_D.7_NER

value: Neutrophils


title: SUB29, B cells, A/H3N2, Day1

description:
Library name: SUB29_D1_BCR
SUB29_D1_BCR

value: B cells


title: SUB29, B cells, A/H3N2, Day-7

description:
Library name: SUB29_D.7_BCR
SUB29_D.7_BCR


title: SUB28, NK cells, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB28_D4_NKR
SUB28_D4_NKR

value: A/H7N9 vaccine unadjuvanted


title: SUB28, T cells, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB28_D4_TCR
SUB28_D4_TCR


title: SUB29, Dendritic cells, A/H3N2, Day-7

description:
Library name: SUB29_D.7_DNR
SUB29_D.7_DNR


title: SUB28, Neutrophils, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB28_D4_NER
SUB28_D4_NER


title: SUB28, Monocytes, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB28_D4_MNR
SUB28_D4_MNR


title: SUB28, B cells, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB28_D4_BCR
SUB28_D4_BCR


title: SUB28, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB28_D4_DNR
SUB28_D4_DNR


title: SUB28, Monocytes, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB28_D36_MNR
SUB28_D36_MNR


title: SUB28, T cells, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB28_D36_TCR
SUB28_D36_TCR


title: SUB28, NK cells, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB28_D36_NKR
SUB28_D36_NKR


title: SH-SY5Y_SR4835_DMSO_rep3

description:
Library name: RNA019390
SH-SY5Y_SR4835_DMSO_rep3

value: DMSO in SR-4835 experiment


title: SUB28, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB28_D36_DNR
SUB28_D36_DNR


title: SUB28, B cells, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB28_D36_BCR
SUB28_D36_BCR


title: SUB28, T cells, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB28_D32_TCR
SUB28_D32_TCR


title: SUB28, NK cells, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB28_D32_NKR
SUB28_D32_NKR


title: SUB28, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB28_D32_DNR
SUB28_D32_DNR


title: SUB28, NK cells, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB28_D30_NKR
SUB28_D30_NKR


title: SUB28, T cells, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB28_D30_TCR
SUB28_D30_TCR


title: SUB28, Monocytes, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB28_D30_MNR
SUB28_D30_MNR


title: SUB28, Neutrophils, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB28_D30_NER
SUB28_D30_NER


title: SUB28, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB28_D30_DNR
SUB28_D30_DNR


title: SUB28, B cells, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB28_D30_BCR
SUB28_D30_BCR


title: SUB28, NK cells, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB28_D2_NKR
SUB28_D2_NKR


title: SUB28, T cells, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB28_D2_TCR
SUB28_D2_TCR


title: SUB28, B cells, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB28_D2_BCR
SUB28_D2_BCR


title: SUB28, Monocytes, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB28_D2_MNR
SUB28_D2_MNR


title: SUB28, Neutrophils, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB28_D29_NER
SUB28_D29_NER


title: SUB28, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB28_D2_DNR
SUB28_D2_DNR


title: SUB28, NK cells, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB28_D29_NKR
SUB28_D29_NKR


title: SUB28, T cells, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB28_D29_TCR
SUB28_D29_TCR


title: SUB28, Monocytes, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB28_D29_MNR
SUB28_D29_MNR


title: SUB28, T cells, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB28_D1_TCR
SUB28_D1_TCR


title: SUB28, B cells, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB28_D29_BCR
SUB28_D29_BCR


title: SUB28, NK cells, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB28_D1_NKR
SUB28_D1_NKR


title: SUB28, Neutrophils, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB28_D1_NER
SUB28_D1_NER


title: SUB28, B cells, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB28_D1_BCR
SUB28_D1_BCR


title: SUB28, T cells, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB28_D.7_TCR
SUB28_D.7_TCR


title: SUB28, NK cells, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB28_D.7_NKR
SUB28_D.7_NKR


title: SUB28, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB28_D.7_DNR
SUB28_D.7_DNR


title: SUB28, Neutrophils, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB28_D.7_NER
SUB28_D.7_NER


title: SUB27, T cells, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB27_D4_TCR
SUB27_D4_TCR

value: A/H7N9 vaccine with AS03 adjuvant


title: SUB27, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB27_D4_NER
SUB27_D4_NER


title: SUB27, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB27_D4_DNR
SUB27_D4_DNR


title: SUB27, B cells, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB27_D4_BCR
SUB27_D4_BCR


title: SUB27, T cells, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB27_D36_TCR
SUB27_D36_TCR


title: SUB27, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB27_D36_NER
SUB27_D36_NER


title: SUB27, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB27_D36_MNR
SUB27_D36_MNR


title: SUB27, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB27_D36_NKR
SUB27_D36_NKR


title: SUB27, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB27_D36_DNR
SUB27_D36_DNR


title: SUB27, T cells, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB27_D32_TCR
SUB27_D32_TCR


title: SUB27, B cells, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB27_D36_BCR
SUB27_D36_BCR


title: SUB27, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB27_D32_NKR
SUB27_D32_NKR


title: SUB27, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB27_D32_NER
SUB27_D32_NER


title: SUB27, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB27_D32_DNR
SUB27_D32_DNR


title: SUB27, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB27_D32_MNR
SUB27_D32_MNR


title: SUB27, B cells, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB27_D32_BCR
SUB27_D32_BCR


title: SUB27, T cells, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB27_D30_TCR
SUB27_D30_TCR


title: SUB27, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB27_D30_NKR
SUB27_D30_NKR


title: SUB27, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB27_D30_NER
SUB27_D30_NER


title: SUB27, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB27_D30_MNR
SUB27_D30_MNR


title: SUB27, B cells, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB27_D30_BCR
SUB27_D30_BCR


title: SUB27, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB27_D30_DNR
SUB27_D30_DNR


title: SUB27, T cells, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB27_D2_TCR
SUB27_D2_TCR


title: SUB27, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB27_D2_NER
SUB27_D2_NER


title: SUB27, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB27_D2_MNR
SUB27_D2_MNR


title: SUB27, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB27_D2_NKR
SUB27_D2_NKR


title: SUB27, B cells, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB27_D2_BCR
SUB27_D2_BCR


title: SUB27, T cells, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB27_D29_TCR
SUB27_D29_TCR


title: SUB27, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB27_D29_NKR
SUB27_D29_NKR


title: SUB27, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB27_D29_NER
SUB27_D29_NER


title: SUB27, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB27_D29_MNR
SUB27_D29_MNR


title: SUB27, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB27_D29_DNR
SUB27_D29_DNR


title: SUB27, B cells, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB27_D29_BCR
SUB27_D29_BCR


title: SUB27, T cells, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB27_D1_TCR
SUB27_D1_TCR


title: SUB27, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB27_D1_NKR
SUB27_D1_NKR


title: SUB27, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB27_D1_MNR
SUB27_D1_MNR


title: SUB27, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB27_D1_NER
SUB27_D1_NER


title: SUB27, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB27_D.7_NKR
SUB27_D.7_NKR


title: SUB27, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB27_D.7_NER
SUB27_D.7_NER


title: SUB27, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB27_D.7_MNR
SUB27_D.7_MNR


title: SUB27, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB27_D.7_DNR
SUB27_D.7_DNR


title: SUB27, B cells, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB27_D.7_BCR
SUB27_D.7_BCR


title: SUB26, T cells, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB26_D4_TCR
SUB26_D4_TCR


title: SUB26, NK cells, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB26_D4_NKR
SUB26_D4_NKR


title: SUB26, Neutrophils, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB26_D4_NER
SUB26_D4_NER


title: SUB26, Monocytes, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB26_D4_MNR
SUB26_D4_MNR


title: SUB26, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB26_D4_DNR
SUB26_D4_DNR


title: SUB26, B cells, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB26_D4_BCR
SUB26_D4_BCR


title: SUB26, T cells, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB26_D36_TCR
SUB26_D36_TCR


title: SUB26, NK cells, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB26_D36_NKR
SUB26_D36_NKR


title: SUB26, Neutrophils, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB26_D36_NER
SUB26_D36_NER


title: SUB27, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB27_D1_DNR
SUB27_D1_DNR


title: SUB26, Monocytes, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB26_D36_MNR
SUB26_D36_MNR


title: SUB27, T cells, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB27_D.7_TCR
SUB27_D.7_TCR


title: SUB26, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB26_D36_DNR
SUB26_D36_DNR


title: SUB27, B cells, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB27_D1_BCR
SUB27_D1_BCR


title: SUB26, B cells, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB26_D36_BCR
SUB26_D36_BCR


title: SUB26, NK cells, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB26_D32_NKR
SUB26_D32_NKR


title: SUB26, T cells, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB26_D32_TCR
SUB26_D32_TCR


title: SUB26, Neutrophils, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB26_D32_NER
SUB26_D32_NER


title: SUB26, Monocytes, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB26_D32_MNR
SUB26_D32_MNR


title: SUB26, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB26_D32_DNR
SUB26_D32_DNR


title: SUB26, B cells, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB26_D32_BCR
SUB26_D32_BCR


title: SUB26, T cells, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB26_D30_TCR
SUB26_D30_TCR


title: SUB26, NK cells, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB26_D30_NKR
SUB26_D30_NKR


title: SUB28, Monocytes, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB28_D32_MNR
SUB28_D32_MNR


title: SUB26, Neutrophils, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB26_D30_NER
SUB26_D30_NER


title: SUB26, Monocytes, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB26_D30_MNR
SUB26_D30_MNR


title: SUB26, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB26_D30_DNR
SUB26_D30_DNR


title: SUB28, B cells, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB28_D32_BCR
SUB28_D32_BCR


title: SUB28, Neutrophils, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB28_D2_NER
SUB28_D2_NER


title: SUB26, B cells, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB26_D30_BCR
SUB26_D30_BCR


title: SUB26, T cells, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB26_D2_TCR
SUB26_D2_TCR


title: SUB26, NK cells, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB26_D2_NKR
SUB26_D2_NKR


title: SUB26, T cells, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB26_D29_TCR
SUB26_D29_TCR


title: SUB26, Monocytes, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB26_D2_MNR
SUB26_D2_MNR


title: SUB26, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB26_D2_DNR
SUB26_D2_DNR


title: SUB26, B cells, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB26_D2_BCR
SUB26_D2_BCR


title: SUB26, Neutrophils, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB26_D2_NER
SUB26_D2_NER


title: SUB28, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB28_D29_DNR
SUB28_D29_DNR


title: SUB28, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB28_D1_DNR
SUB28_D1_DNR


title: SUB28, Monocytes, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB28_D1_MNR
SUB28_D1_MNR


title: SUB26, NK cells, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB26_D29_NKR
SUB26_D29_NKR


title: SUB26, Neutrophils, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB26_D29_NER
SUB26_D29_NER


title: SUB28, Monocytes, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB28_D.7_MNR
SUB28_D.7_MNR


title: SUB26, Monocytes, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB26_D29_MNR
SUB26_D29_MNR


title: SUB26, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB26_D29_DNR
SUB26_D29_DNR


title: SUB26, T cells, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB26_D1_TCR
SUB26_D1_TCR


title: SUB26, NK cells, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB26_D1_NKR
SUB26_D1_NKR


title: SUB26, B cells, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB26_D29_BCR
SUB26_D29_BCR


title: SUB26, Monocytes, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB26_D1_MNR
SUB26_D1_MNR


title: SUB26, Neutrophils, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB26_D1_NER
SUB26_D1_NER


title: SUB27, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB27_D4_NKR
SUB27_D4_NKR


title: SUB28, B cells, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB28_D.7_BCR
SUB28_D.7_BCR


title: SUB26, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB26_D1_DNR
SUB26_D1_DNR


title: SUB27, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB27_D4_MNR
SUB27_D4_MNR


title: SUB26, B cells, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB26_D1_BCR
SUB26_D1_BCR


title: SUB26, NK cells, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB26_D.7_NKR
SUB26_D.7_NKR


title: SUB26, Monocytes, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB26_D.7_MNR
SUB26_D.7_MNR


title: SUB26, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB26_D.7_DNR
SUB26_D.7_DNR


title: SUB26, B cells, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB26_D.7_BCR
SUB26_D.7_BCR


title: SUB25, Neutrophils, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB25_D4_NER
SUB25_D4_NER


title: SUB25, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB25_D4_DNR
SUB25_D4_DNR


title: SUB25, NK cells, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB25_D4_NKR
SUB25_D4_NKR


title: SUB25, B cells, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB25_D4_BCR
SUB25_D4_BCR


title: SUB25, Monocytes, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB25_D4_MNR
SUB25_D4_MNR


title: SUB25, Neutrophils, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB25_D36_NER
SUB25_D36_NER


title: SUB25, Monocytes, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB25_D36_MNR
SUB25_D36_MNR


title: SUB25, B cells, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB25_D36_BCR
SUB25_D36_BCR


title: SUB25, NK cells, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB25_D36_NKR
SUB25_D36_NKR


title: SUB25, T cells, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB25_D32_TCR
SUB25_D32_TCR


title: SUB25, NK cells, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB25_D32_NKR
SUB25_D32_NKR


title: SUB25, Monocytes, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB25_D32_MNR
SUB25_D32_MNR


title: SUB25, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB25_D32_DNR
SUB25_D32_DNR


title: SUB25, B cells, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB25_D32_BCR
SUB25_D32_BCR


title: SUB25, Neutrophils, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB25_D30_NER
SUB25_D30_NER


title: SUB25, NK cells, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB25_D30_NKR
SUB25_D30_NKR


title: SUB25, B cells, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB25_D30_BCR
SUB25_D30_BCR


title: SUB25, T cells, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB25_D2_TCR
SUB25_D2_TCR


title: SUB25, Neutrophils, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB25_D2_NER
SUB25_D2_NER


title: SUB25, Monocytes, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB25_D2_MNR
SUB25_D2_MNR


title: SUB25, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB25_D2_DNR
SUB25_D2_DNR


title: SUB25, T cells, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB25_D29_TCR
SUB25_D29_TCR


title: SUB25, B cells, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB25_D2_BCR
SUB25_D2_BCR


title: SUB25, NK cells, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB25_D29_NKR
SUB25_D29_NKR


title: SUB25, Neutrophils, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB25_D29_NER
SUB25_D29_NER


title: SUB25, Monocytes, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB25_D29_MNR
SUB25_D29_MNR


title: SUB25, T cells, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB25_D1_TCR
SUB25_D1_TCR


title: SUB25, NK cells, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB25_D1_NKR
SUB25_D1_NKR


title: SUB25, Monocytes, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB25_D1_MNR
SUB25_D1_MNR


title: SUB25, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB25_D1_DNR
SUB25_D1_DNR


title: SUB25, B cells, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB25_D1_BCR
SUB25_D1_BCR


title: SUB25, T cells, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB25_D.7_TCR
SUB25_D.7_TCR


title: SUB25, NK cells, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB25_D.7_NKR
SUB25_D.7_NKR


title: SUB25, Neutrophils, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB25_D.7_NER
SUB25_D.7_NER


title: SUB25, Monocytes, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB25_D.7_MNR
SUB25_D.7_MNR


title: SUB24, NK cells, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB24_D4_NKR
SUB24_D4_NKR


title: SUB24, Neutrophils, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB24_D4_NER
SUB24_D4_NER


title: SUB24, Monocytes, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB24_D4_MNR
SUB24_D4_MNR


title: SUB24, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB24_D4_DNR
SUB24_D4_DNR


title: SUB26, T cells, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB26_D.7_TCR
SUB26_D.7_TCR


title: SUB25, T cells, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB25_D36_TCR
SUB25_D36_TCR


title: SUB24, B cells, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB24_D4_BCR
SUB24_D4_BCR


title: SUB25, T cells, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB25_D4_TCR
SUB25_D4_TCR


title: SUB25, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB25_D36_DNR
SUB25_D36_DNR


title: SUB24, T cells, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB24_D36_TCR
SUB24_D36_TCR


title: SUB25, Neutrophils, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB25_D32_NER
SUB25_D32_NER


title: SUB25, T cells, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB25_D30_TCR
SUB25_D30_TCR


title: SUB24, NK cells, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB24_D36_NKR
SUB24_D36_NKR


title: SUB24, Monocytes, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB24_D36_MNR
SUB24_D36_MNR


title: SUB24, Neutrophils, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB24_D36_NER
SUB24_D36_NER


title: SUB25, Monocytes, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB25_D30_MNR
SUB25_D30_MNR


title: SUB24, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB24_D36_DNR
SUB24_D36_DNR


title: SUB24, B cells, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB24_D36_BCR
SUB24_D36_BCR


title: SUB25, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB25_D30_DNR
SUB25_D30_DNR


title: SUB25, NK cells, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB25_D2_NKR
SUB25_D2_NKR


title: SUB24, T cells, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB24_D32_TCR
SUB24_D32_TCR


title: SUB24, Neutrophils, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB24_D32_NER
SUB24_D32_NER


title: SUB24, Monocytes, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB24_D32_MNR
SUB24_D32_MNR


title: SUB24, NK cells, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB24_D32_NKR
SUB24_D32_NKR


title: SUB24, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB24_D32_DNR
SUB24_D32_DNR


title: SUB24, B cells, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB24_D32_BCR
SUB24_D32_BCR


title: SUB24, T cells, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB24_D30_TCR
SUB24_D30_TCR


title: SUB24, NK cells, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB24_D30_NKR
SUB24_D30_NKR


title: SUB24, Neutrophils, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB24_D30_NER
SUB24_D30_NER


title: SUB25, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB25_D29_DNR
SUB25_D29_DNR


title: SUB25, B cells, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB25_D29_BCR
SUB25_D29_BCR


title: SUB24, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB24_D30_DNR
SUB24_D30_DNR


title: SUB24, Monocytes, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB24_D30_MNR
SUB24_D30_MNR


title: SUB24, Neutrophils, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB24_D2_NER
SUB24_D2_NER


title: SUB24, T cells, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB24_D2_TCR
SUB24_D2_TCR


title: SUB24, Monocytes, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB24_D2_MNR
SUB24_D2_MNR


title: SUB24, NK cells, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB24_D2_NKR
SUB24_D2_NKR


title: SUB24, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB24_D2_DNR
SUB24_D2_DNR


title: SUB24, B cells, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB24_D2_BCR
SUB24_D2_BCR


title: SUB24, T cells, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB24_D29_TCR
SUB24_D29_TCR


title: SUB25, Neutrophils, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB25_D1_NER
SUB25_D1_NER


title: SUB24, NK cells, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB24_D29_NKR
SUB24_D29_NKR


title: SUB24, Neutrophils, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB24_D29_NER
SUB24_D29_NER


title: SUB24, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB24_D29_DNR
SUB24_D29_DNR


title: SUB24, T cells, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB24_D1_TCR
SUB24_D1_TCR


title: SUB24, Neutrophils, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB24_D1_NER
SUB24_D1_NER


title: SUB24, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB24_D1_DNR
SUB24_D1_DNR


title: SUB24, T cells, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB24_D.7_TCR
SUB24_D.7_TCR


title: SUB24, Neutrophils, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB24_D.7_NER
SUB24_D.7_NER


title: SUB24, NK cells, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB24_D.7_NKR
SUB24_D.7_NKR


title: SUB24, Monocytes, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB24_D.7_MNR
SUB24_D.7_MNR


title: SUB24, B cells, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB24_D1_BCR
SUB24_D1_BCR


title: SUB24, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB24_D.7_DNR
SUB24_D.7_DNR


title: SUB24, B cells, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB24_D.7_BCR
SUB24_D.7_BCR


title: SUB23, T cells, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB23_D4_TCR
SUB23_D4_TCR


title: SUB23, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB23_D4_NKR
SUB23_D4_NKR


title: SUB23, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB23_D4_NER
SUB23_D4_NER


title: SUB23, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB23_D4_MNR
SUB23_D4_MNR


title: SUB23, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB23_D4_DNR
SUB23_D4_DNR


title: SUB23, T cells, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB23_D36_TCR
SUB23_D36_TCR


title: SUB23, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB23_D36_NKR
SUB23_D36_NKR


title: SUB23, B cells, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB23_D4_BCR
SUB23_D4_BCR


title: SUB23, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB23_D36_NER
SUB23_D36_NER


title: SUB23, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB23_D36_MNR
SUB23_D36_MNR


title: SUB23, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB23_D36_DNR
SUB23_D36_DNR


title: SUB23, B cells, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB23_D36_BCR
SUB23_D36_BCR


title: SUB23, T cells, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB23_D32_TCR
SUB23_D32_TCR


title: SUB23, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB23_D32_NKR
SUB23_D32_NKR


title: SUB23, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB23_D32_MNR
SUB23_D32_MNR


title: SUB23, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB23_D32_NER
SUB23_D32_NER


title: SUB23, B cells, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB23_D32_BCR
SUB23_D32_BCR


title: SUB23, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB23_D30_NKR
SUB23_D30_NKR


title: SUB23, T cells, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB23_D30_TCR
SUB23_D30_TCR


title: SUB23, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB23_D30_NER
SUB23_D30_NER


title: SUB06, Monocytes, A/H3N2, Day4

description:
Library name: SUB06_D4_MNR
SUB06_D4_MNR


title: SUB06, B cells, A/H3N2, Day4

description:
Library name: SUB06_D4_BCR
SUB06_D4_BCR


title: SUB06, Dendritic cells, A/H3N2, Day4

description:
Library name: SUB06_D4_DNR
SUB06_D4_DNR


title: SUB06, T cells, A/H3N2, Day2

description:
Library name: SUB06_D2_TCR
SUB06_D2_TCR


title: SUB06, NK cells, A/H3N2, Day2

description:
Library name: SUB06_D2_NKR
SUB06_D2_NKR


title: SUB06, Neutrophils, A/H3N2, Day2

description:
Library name: SUB06_D2_NER
SUB06_D2_NER


title: SUB06, Monocytes, A/H3N2, Day2

description:
Library name: SUB06_D2_MNR
SUB06_D2_MNR


title: SUB06, Dendritic cells, A/H3N2, Day2

description:
Library name: SUB06_D2_DNR
SUB06_D2_DNR


title: SUB06, B cells, A/H3N2, Day2

description:
Library name: SUB06_D2_BCR
SUB06_D2_BCR


title: SUB06, T cells, A/H3N2, Day1

description:
Library name: SUB06_D1_TCR
SUB06_D1_TCR


title: SUB06, NK cells, A/H3N2, Day1

description:
Library name: SUB06_D1_NKR
SUB06_D1_NKR


title: SUB06, Neutrophils, A/H3N2, Day1

description:
Library name: SUB06_D1_NER
SUB06_D1_NER


title: SUB06, Monocytes, A/H3N2, Day1

description:
Library name: SUB06_D1_MNR
SUB06_D1_MNR


title: SUB06, Dendritic cells, A/H3N2, Day1

description:
Library name: SUB06_D1_DNR
SUB06_D1_DNR


title: SUB06, B cells, A/H3N2, Day1

description:
Library name: SUB06_D1_BCR
SUB06_D1_BCR


title: SUB06, T cells, A/H3N2, Day-7

description:
Library name: SUB06_D.7_TCR
SUB06_D.7_TCR


title: SUB06, NK cells, A/H3N2, Day-7

description:
Library name: SUB06_D.7_NKR
SUB06_D.7_NKR


title: SUB24, B cells, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB24_D29_BCR
SUB24_D29_BCR


title: SUB24, NK cells, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB24_D1_NKR
SUB24_D1_NKR


title: SUB06, Neutrophils, A/H3N2, Day-7

description:
Library name: SUB06_D.7_NER
SUB06_D.7_NER


title: SUB24, Monocytes, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB24_D1_MNR
SUB24_D1_MNR


title: SUB06, Monocytes, A/H3N2, Day-7

description:
Library name: SUB06_D.7_MNR
SUB06_D.7_MNR


title: SUB06, Dendritic cells, A/H3N2, Day-7

description:
Library name: SUB06_D.7_DNR
SUB06_D.7_DNR


title: SUB06, B cells, A/H3N2, Day-7

description:
Library name: SUB06_D.7_BCR
SUB06_D.7_BCR


title: SUB05, T cells, A/H3N2, Day8

description:
Library name: SUB05_D8_TCR
SUB05_D8_TCR


title: SUB05, Neutrophils, A/H3N2, Day8

description:
Library name: SUB05_D8_NER
SUB05_D8_NER


title: SUB05, NK cells, A/H3N2, Day8

description:
Library name: SUB05_D8_NKR
SUB05_D8_NKR


title: SUB05, Monocytes, A/H3N2, Day8

description:
Library name: SUB05_D8_MNR
SUB05_D8_MNR


title: SUB05, Dendritic cells, A/H3N2, Day8

description:
Library name: SUB05_D8_DNR
SUB05_D8_DNR


title: SUB05, B cells, A/H3N2, Day8

description:
Library name: SUB05_D8_BCR
SUB05_D8_BCR


title: SUB05, Neutrophils, A/H3N2, Day4

description:
Library name: SUB05_D4_NER
SUB05_D4_NER


title: SUB05, NK cells, A/H3N2, Day4

description:
Library name: SUB05_D4_NKR
SUB05_D4_NKR


title: SUB05, T cells, A/H3N2, Day4

description:
Library name: SUB05_D4_TCR
SUB05_D4_TCR


title: SUB05, Monocytes, A/H3N2, Day4

description:
Library name: SUB05_D4_MNR
SUB05_D4_MNR


title: SUB05, Dendritic cells, A/H3N2, Day4

description:
Library name: SUB05_D4_DNR
SUB05_D4_DNR


title: SUB05, B cells, A/H3N2, Day4

description:
Library name: SUB05_D4_BCR
SUB05_D4_BCR


title: SUB05, T cells, A/H3N2, Day2

description:
Library name: SUB05_D2_TCR
SUB05_D2_TCR


title: SUB05, NK cells, A/H3N2, Day2

description:
Library name: SUB05_D2_NKR
SUB05_D2_NKR


title: SUB05, Neutrophils, A/H3N2, Day2

description:
Library name: SUB05_D2_NER
SUB05_D2_NER


title: SUB05, Monocytes, A/H3N2, Day2

description:
Library name: SUB05_D2_MNR
SUB05_D2_MNR


title: SUB05, Dendritic cells, A/H3N2, Day2

description:
Library name: SUB05_D2_DNR
SUB05_D2_DNR


title: SUB05, B cells, A/H3N2, Day2

description:
Library name: SUB05_D2_BCR
SUB05_D2_BCR


title: SUB05, T cells, A/H3N2, Day1

description:
Library name: SUB05_D1_TCR
SUB05_D1_TCR


title: SUB05, Neutrophils, A/H3N2, Day1

description:
Library name: SUB05_D1_NER
SUB05_D1_NER


title: SUB05, Monocytes, A/H3N2, Day1

description:
Library name: SUB05_D1_MNR
SUB05_D1_MNR


title: SUB23, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB23_D32_DNR
SUB23_D32_DNR


title: SUB05, Dendritic cells, A/H3N2, Day1

description:
Library name: SUB05_D1_DNR
SUB05_D1_DNR


title: SUB05, B cells, A/H3N2, Day1

description:
Library name: SUB05_D1_BCR
SUB05_D1_BCR


title: SUB06, Neutrophils, A/H3N2, Day4

description:
Library name: SUB06_D4_NER
SUB06_D4_NER


title: SUB05, T cells, A/H3N2, Day-7

description:
Library name: SUB05_D.7_TCR
SUB05_D.7_TCR


title: SUB05, NK cells, A/H3N2, Day-7

description:
Library name: SUB05_D.7_NKR
SUB05_D.7_NKR


title: SUB05, Neutrophils, A/H3N2, Day-7

description:
Library name: SUB05_D.7_NER
SUB05_D.7_NER


title: SUB05, Monocytes, A/H3N2, Day-7

description:
Library name: SUB05_D.7_MNR
SUB05_D.7_MNR


title: SUB05, B cells, A/H3N2, Day-7

description:
Library name: SUB05_D.7_BCR
SUB05_D.7_BCR


title: SUB05, Dendritic cells, A/H3N2, Day-7

description:
Library name: SUB05_D.7_DNR
SUB05_D.7_DNR


title: SUB04, T cells, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB04_D4_TCR
SUB04_D4_TCR


title: SUB04, NK cells, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB04_D4_NKR
SUB04_D4_NKR


title: SUB04, Neutrophils, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB04_D4_NER
SUB04_D4_NER


title: SUB04, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB04_D4_DNR
SUB04_D4_DNR


title: SUB04, Monocytes, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB04_D4_MNR
SUB04_D4_MNR


title: SUB05, NK cells, A/H3N2, Day1

description:
Library name: SUB05_D1_NKR
SUB05_D1_NKR


title: SUB04, NK cells, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB04_D36_NKR
SUB04_D36_NKR


title: SUB04, T cells, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB04_D36_TCR
SUB04_D36_TCR


title: SUB04, B cells, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB04_D4_BCR
SUB04_D4_BCR


title: SUB04, Neutrophils, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB04_D36_NER
SUB04_D36_NER


title: SUB04, Monocytes, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB04_D36_MNR
SUB04_D36_MNR


title: SUB04, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB04_D36_DNR
SUB04_D36_DNR


title: SUB04, B cells, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB04_D36_BCR
SUB04_D36_BCR


title: SUB04, T cells, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB04_D32_TCR
SUB04_D32_TCR


title: SUB04, Neutrophils, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB04_D32_NER
SUB04_D32_NER


title: SUB04, NK cells, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB04_D32_NKR
SUB04_D32_NKR


title: SUB04, Monocytes, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB04_D32_MNR
SUB04_D32_MNR


title: SUB04, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB04_D32_DNR
SUB04_D32_DNR


title: SUB04, B cells, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB04_D32_BCR
SUB04_D32_BCR


title: SUB04, NK cells, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB04_D30_NKR
SUB04_D30_NKR


title: SUB04, Neutrophils, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB04_D30_NER
SUB04_D30_NER


title: SUB04, Monocytes, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB04_D30_MNR
SUB04_D30_MNR


title: SUB04, T cells, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB04_D30_TCR
SUB04_D30_TCR


title: SUB04, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB04_D30_DNR
SUB04_D30_DNR


title: SUB04, B cells, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB04_D30_BCR
SUB04_D30_BCR


title: SUB04, T cells, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB04_D2_TCR
SUB04_D2_TCR


title: SUB04, NK cells, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB04_D2_NKR
SUB04_D2_NKR


title: SUB04, Monocytes, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB04_D2_MNR
SUB04_D2_MNR


title: SUB04, Neutrophils, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB04_D2_NER
SUB04_D2_NER


title: SUB04, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB04_D2_DNR
SUB04_D2_DNR


title: SUB04, B cells, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB04_D2_BCR
SUB04_D2_BCR


title: SUB04, NK cells, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB04_D29_NKR
SUB04_D29_NKR


title: SUB04, Neutrophils, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB04_D29_NER
SUB04_D29_NER


title: SUB04, Monocytes, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB04_D29_MNR
SUB04_D29_MNR


title: SUB04, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB04_D29_DNR
SUB04_D29_DNR


title: SUB04, T cells, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB04_D29_TCR
SUB04_D29_TCR


title: SUB04, B cells, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB04_D29_BCR
SUB04_D29_BCR


title: SUB04, NK cells, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB04_D1_NKR
SUB04_D1_NKR


title: SUB04, T cells, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB04_D1_TCR
SUB04_D1_TCR


title: SUB04, Neutrophils, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB04_D1_NER
SUB04_D1_NER


title: SUB04, Monocytes, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB04_D1_MNR
SUB04_D1_MNR


title: SUB04, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB04_D1_DNR
SUB04_D1_DNR


title: SUB04, B cells, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB04_D1_BCR
SUB04_D1_BCR


title: SUB04, T cells, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB04_D.7_TCR
SUB04_D.7_TCR


title: SUB04, NK cells, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB04_D.7_NKR
SUB04_D.7_NKR


title: SUB04, Neutrophils, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB04_D.7_NER
SUB04_D.7_NER


title: SUB04, Monocytes, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB04_D.7_MNR
SUB04_D.7_MNR


title: SUB04, B cells, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB04_D.7_BCR
SUB04_D.7_BCR


title: SUB03, T cells, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB03_D4_TCR
SUB03_D4_TCR


title: SUB03, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB03_D4_NKR
SUB03_D4_NKR


title: SUB04, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB04_D.7_DNR
SUB04_D.7_DNR


title: SUB03, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB03_D4_MNR
SUB03_D4_MNR


title: SUB03, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB03_D4_NER
SUB03_D4_NER


title: SUB03, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB03_D4_DNR
SUB03_D4_DNR


title: SUB03, B cells, A/H7N9 vaccine with AS03 adjuvant, Day4

description:
Library name: SUB03_D4_BCR
SUB03_D4_BCR


title: SUB03, T cells, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB03_D36_TCR
SUB03_D36_TCR


title: SUB03, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB03_D36_DNR
SUB03_D36_DNR


title: SUB03, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB03_D36_NKR
SUB03_D36_NKR


title: SUB03, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB03_D36_MNR
SUB03_D36_MNR


title: SUB03, B cells, A/H7N9 vaccine with AS03 adjuvant, Day36

description:
Library name: SUB03_D36_BCR
SUB03_D36_BCR


title: SUB03, T cells, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB03_D32_TCR
SUB03_D32_TCR


title: SUB03, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB03_D32_MNR
SUB03_D32_MNR


title: SUB03, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB03_D32_NKR
SUB03_D32_NKR


title: SUB03, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB03_D32_DNR
SUB03_D32_DNR


title: SUB03, B cells, A/H7N9 vaccine with AS03 adjuvant, Day32

description:
Library name: SUB03_D32_BCR
SUB03_D32_BCR


title: SUB03, T cells, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB03_D30_TCR
SUB03_D30_TCR


title: SUB03, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB03_D30_NKR
SUB03_D30_NKR


title: SUB03, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB03_D30_NER
SUB03_D30_NER


title: SUB03, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB03_D30_MNR
SUB03_D30_MNR


title: SUB03, T cells, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB03_D2_TCR
SUB03_D2_TCR


title: SUB03, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB03_D2_NKR
SUB03_D2_NKR


title: SUB03, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB03_D30_DNR
SUB03_D30_DNR


title: SUB03, B cells, A/H7N9 vaccine with AS03 adjuvant, Day30

description:
Library name: SUB03_D30_BCR
SUB03_D30_BCR


title: SUB03, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB03_D2_NER
SUB03_D2_NER


title: SUB03, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB03_D2_MNR
SUB03_D2_MNR


title: SUB03, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB03_D2_DNR
SUB03_D2_DNR


title: SUB03, B cells, A/H7N9 vaccine with AS03 adjuvant, Day2

description:
Library name: SUB03_D2_BCR
SUB03_D2_BCR


title: SUB03, T cells, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB03_D29_TCR
SUB03_D29_TCR


title: SUB03, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB03_D29_NKR
SUB03_D29_NKR


title: SUB03, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB03_D29_NER
SUB03_D29_NER


title: SUB03, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB03_D29_MNR
SUB03_D29_MNR


title: SUB03, B cells, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB03_D29_BCR
SUB03_D29_BCR


title: SUB03, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB03_D1_NKR
SUB03_D1_NKR


title: SUB03, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB03_D1_MNR
SUB03_D1_MNR


title: SUB03, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day29

description:
Library name: SUB03_D29_DNR
SUB03_D29_DNR


title: SUB03, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB03_D1_NER
SUB03_D1_NER


title: SUB03, T cells, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB03_D1_TCR
SUB03_D1_TCR


title: SUB03, B cells, A/H7N9 vaccine with AS03 adjuvant, Day1

description:
Library name: SUB03_D1_BCR
SUB03_D1_BCR


title: SUB03, T cells, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB03_D.7_TCR
SUB03_D.7_TCR


title: SUB03, Neutrophils, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB03_D.7_NER
SUB03_D.7_NER


title: SUB03, Monocytes, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB03_D.7_MNR
SUB03_D.7_MNR


title: SUB03, Dendritic cells, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB03_D.7_DNR
SUB03_D.7_DNR


title: SUB03, B cells, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB03_D.7_BCR
SUB03_D.7_BCR


title: SUB03, NK cells, A/H7N9 vaccine with AS03 adjuvant, Day-7

description:
Library name: SUB03_D.7_NKR
SUB03_D.7_NKR


title: SUB02, T cells, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB02_D4_TCR
SUB02_D4_TCR


title: SUB02, NK cells, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB02_D4_NKR
SUB02_D4_NKR


title: SUB02, Neutrophils, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB02_D4_NER
SUB02_D4_NER


title: SUB02, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB02_D4_DNR
SUB02_D4_DNR


title: SUB02, Monocytes, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB02_D4_MNR
SUB02_D4_MNR


title: SUB02, B cells, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB02_D4_BCR
SUB02_D4_BCR


title: SUB02, T cells, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB02_D36_TCR
SUB02_D36_TCR


title: SUB02, NK cells, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB02_D36_NKR
SUB02_D36_NKR


title: SUB02, Neutrophils, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB02_D36_NER
SUB02_D36_NER


title: SUB02, Monocytes, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB02_D36_MNR
SUB02_D36_MNR


title: SUB02, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB02_D36_DNR
SUB02_D36_DNR


title: SUB02, B cells, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB02_D36_BCR
SUB02_D36_BCR


title: SUB02, T cells, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB02_D32_TCR
SUB02_D32_TCR


title: SUB02, Monocytes, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB02_D32_MNR
SUB02_D32_MNR


title: SUB02, NK cells, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB02_D32_NKR
SUB02_D32_NKR


title: SUB02, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB02_D32_DNR
SUB02_D32_DNR


title: SUB02, B cells, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB02_D32_BCR
SUB02_D32_BCR


title: SUB02, Neutrophils, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB02_D30_NER
SUB02_D30_NER


title: SUB02, NK cells, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB02_D30_NKR
SUB02_D30_NKR


title: SUB02, T cells, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB02_D30_TCR
SUB02_D30_TCR


title: SUB02, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB02_D30_DNR
SUB02_D30_DNR


title: SUB02, Monocytes, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB02_D30_MNR
SUB02_D30_MNR


title: SUB02, B cells, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB02_D30_BCR
SUB02_D30_BCR


title: SUB02, T cells, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB02_D2_TCR
SUB02_D2_TCR


title: SUB02, Neutrophils, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB02_D2_NER
SUB02_D2_NER


title: SUB02, NK cells, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB02_D2_NKR
SUB02_D2_NKR


title: SUB02, Monocytes, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB02_D2_MNR
SUB02_D2_MNR


title: SUB02, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB02_D2_DNR
SUB02_D2_DNR


title: SUB02, B cells, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB02_D2_BCR
SUB02_D2_BCR


title: SUB02, T cells, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB02_D29_TCR
SUB02_D29_TCR


title: SUB02, Neutrophils, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB02_D29_NER
SUB02_D29_NER


title: SUB02, NK cells, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB02_D29_NKR
SUB02_D29_NKR


title: SUB02, Monocytes, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB02_D29_MNR
SUB02_D29_MNR


title: SUB02, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB02_D29_DNR
SUB02_D29_DNR


title: SUB02, B cells, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB02_D29_BCR
SUB02_D29_BCR


title: SUB02, T cells, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB02_D1_TCR
SUB02_D1_TCR


title: SUB02, NK cells, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB02_D1_NKR
SUB02_D1_NKR


title: SUB02, Monocytes, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB02_D1_MNR
SUB02_D1_MNR


title: SUB02, B cells, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB02_D1_BCR
SUB02_D1_BCR


title: SUB02, Neutrophils, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB02_D1_NER
SUB02_D1_NER


title: SUB02, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB02_D1_DNR
SUB02_D1_DNR


title: SUB02, NK cells, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB02_D.7_NKR
SUB02_D.7_NKR


title: SUB02, Neutrophils, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB02_D.7_NER
SUB02_D.7_NER


title: SUB02, Monocytes, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB02_D.7_MNR
SUB02_D.7_MNR


title: SUB02, T cells, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB02_D.7_TCR
SUB02_D.7_TCR


title: SUB02, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB02_D.7_DNR
SUB02_D.7_DNR


title: SUB02, B cells, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB02_D.7_BCR
SUB02_D.7_BCR


title: SUB01, T cells, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB01_D4_TCR
SUB01_D4_TCR


title: SUB01, NK cells, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB01_D4_NKR
SUB01_D4_NKR


title: SUB01, Neutrophils, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB01_D4_NER
SUB01_D4_NER


title: SUB01, Monocytes, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB01_D4_MNR
SUB01_D4_MNR


title: SUB01, B cells, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB01_D4_BCR
SUB01_D4_BCR


title: SUB01, T cells, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB01_D36_TCR
SUB01_D36_TCR


title: SUB01, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day4

description:
Library name: SUB01_D4_DNR
SUB01_D4_DNR


title: SUB01, NK cells, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB01_D36_NKR
SUB01_D36_NKR


title: SUB01, Neutrophils, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB01_D36_NER
SUB01_D36_NER


title: SUB01, Monocytes, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB01_D36_MNR
SUB01_D36_MNR


title: SUB01, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB01_D36_DNR
SUB01_D36_DNR


title: SUB01, B cells, A/H7N9 vaccine unadjuvanted, Day36

description:
Library name: SUB01_D36_BCR
SUB01_D36_BCR


title: SUB01, T cells, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB01_D32_TCR
SUB01_D32_TCR


title: SUB01, NK cells, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB01_D32_NKR
SUB01_D32_NKR


title: SUB01, Neutrophils, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB01_D32_NER
SUB01_D32_NER


title: SUB01, Monocytes, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB01_D32_MNR
SUB01_D32_MNR


title: SUB01, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB01_D32_DNR
SUB01_D32_DNR


title: SUB01, B cells, A/H7N9 vaccine unadjuvanted, Day32

description:
Library name: SUB01_D32_BCR
SUB01_D32_BCR


title: SUB01, T cells, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB01_D30_TCR
SUB01_D30_TCR


title: SUB01, NK cells, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB01_D30_NKR
SUB01_D30_NKR


title: SUB01, Neutrophils, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB01_D30_NER
SUB01_D30_NER


title: SUB01, B cells, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB01_D30_BCR
SUB01_D30_BCR


title: SUB01, Monocytes, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB01_D30_MNR
SUB01_D30_MNR


title: SUB01, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day30

description:
Library name: SUB01_D30_DNR
SUB01_D30_DNR


title: SUB01, T cells, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB01_D2_TCR
SUB01_D2_TCR


title: SUB01, NK cells, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB01_D2_NKR
SUB01_D2_NKR


title: SUB01, Neutrophils, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB01_D2_NER
SUB01_D2_NER


title: SUB01, Monocytes, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB01_D2_MNR
SUB01_D2_MNR


title: SUB01, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB01_D2_DNR
SUB01_D2_DNR


title: SUB01, B cells, A/H7N9 vaccine unadjuvanted, Day2

description:
Library name: SUB01_D2_BCR
SUB01_D2_BCR


title: SUB01, NK cells, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB01_D29_NKR
SUB01_D29_NKR


title: SUB01, Neutrophils, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB01_D29_NER
SUB01_D29_NER


title: SUB01, Monocytes, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB01_D29_MNR
SUB01_D29_MNR


title: SUB01, T cells, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB01_D29_TCR
SUB01_D29_TCR


title: SUB01, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB01_D29_DNR
SUB01_D29_DNR


title: SUB01, T cells, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB01_D1_TCR
SUB01_D1_TCR


title: SUB01, B cells, A/H7N9 vaccine unadjuvanted, Day29

description:
Library name: SUB01_D29_BCR
SUB01_D29_BCR


title: SUB01, Neutrophils, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB01_D1_NER
SUB01_D1_NER


title: SUB01, NK cells, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB01_D1_NKR
SUB01_D1_NKR


title: SUB01, Monocytes, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB01_D1_MNR
SUB01_D1_MNR


title: N206_SR4835_DMSO_rep2

description:
Library name: RNA019357
N206_SR4835_DMSO_rep2


title: SUB01, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB01_D1_DNR
SUB01_D1_DNR


title: SUB01, B cells, A/H7N9 vaccine unadjuvanted, Day1

description:
Library name: SUB01_D1_BCR
SUB01_D1_BCR


title: SUB01, T cells, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB01_D.7_TCR
SUB01_D.7_TCR


title: SUB01, NK cells, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB01_D.7_NKR
SUB01_D.7_NKR


title: SUB01, Neutrophils, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB01_D.7_NER
SUB01_D.7_NER


title: SUB01, Monocytes, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB01_D.7_MNR
SUB01_D.7_MNR


title: SUB01, Dendritic cells, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB01_D.7_DNR
SUB01_D.7_DNR


title: NTC_T_3

city: Omaha

first: Jordan

last: Rowley

state: NE

address: 3828 Jones St, Apt 202

institute: University of Nebraska Medical Center

description: Library name: Sample 6

data_processing:
RNAseq Reads were aligned and mapped to reference genome using STAR
Peaks from bigwig files were visualized using IGV
TPM values were calculated using DESeq2
Assembly: hg38
Supplementary files format and content: Matrix table with raw gene counts for every gene and every sample and Deseqresults file for RNAseq
Supplementary files format and content: peaks and bigwig for eCLIP data

extract_protocol:
Total RNA was isolated using the Trizol reagent from control or ECD depleted 76NTERT cells. The quality of purified RNA samples was determined using a Bioanalyzer
RNA-seq libraries were prepared using the TruSeq RNA Library Prep Kit v2 (Illumina) following the manufacturer's protocols and subjected to 100 bp paired-end sequencing on an Illumina NovaSeq 6000 system at the UNMC next generation sequencing facility.

treatment_protocol: The ECD (GeneID: 11319) CDS in the mammalian expression vector pLenti-C-Myc-DDK-P2A-Puro and empty Vector control plasmids were obtained from (Origene Biotech cat # PS100092). RNA binding region was deleted from WT ECD containing plasmid using PCR-based site-directed mutagenesis as mentioned above. Lentiviral supernatants were generated by transient co-transfection of Lenti-X 293T cells with lentiviral packaging plasmid, psPAX2 (Addgene, #12260) envelope pMD2.G plasmid (Addgene#12259) and either empty mammalian expression vector or ECD (WT and mutant) CDS carrying vectors using X-treme GENE HP DNA transfection reagent (Roche#06366236001). 76NTERT cells were transduced with collected lentiviral supernatant in the presence of polybrene (8 µg/ml, Sigma#H9268). 72 hours post-transduction, cells were selected in 1 μg/ml puromycin for 5 days, and ECD levels assessed by Western blotting (WB) using an anti-ECD monoclonal antibody (Kim et al., 2009).

value: mammary epithelial cells from normal breast tissue


title: SUB01, B cells, A/H7N9 vaccine unadjuvanted, Day-7

description:
Library name: SUB01_D.7_BCR
SUB01_D.7_BCR


title: NTC_T_2

description: Library name: Sample 5


title: ECD_sg_T_3

description: Library name: Sample 3


title: NTC_T_1

description: Library name: Sample 4


title: ECD_sg_T_2

description: Library name: Sample 2


title: ECD_sg_T_1

description: Library name: Sample 1


title: HeLa cells, Total RNA-seq, 12A, rep1

city: Beijing

first: Qidong

last: Deng

address: No.5 Yiheyuan Road Haidian District, Beijing 100871 P.R. China

department: School of Life Sciences

institute: Peking university

data_processing:
For RNA-seq analysis, raw sequencing reads for shBRD4 HeLa rescued by EGFP-BRD4-WT and EGFP-BRD4-12A samples were trimmed for low-quality nucleotides and adapter sequences using Trim Galore! (v0.6.7). The reads were then aligned to human genome GRCh38/hg38 with HISAT2 (v2.2.1) using the ‘-rna-strandness RF’ parameter and an additional ‘-k 30’ parameter for chromatin RNA analysis. Gene annotation GTF format files for human were downloaded from the ENSEMBL database (https://www.ensembl.org/index.html). Reads counts for genes were obtained using featureCounts (v2.0.2) and then normalized to fragments per kilobase of transcript per million mapped reads (FPKM). Differential expression analysis was performed with the R/Bioconductor package DESeq2 (v1.36.0),using P value < 0.05 and log2 |fold change| > log2 (1.5) threshold.
For ChIP-seq analysis, raw reads were trimmed and aligned to the GRCh38/hg38 human reference genome using Bowtie2 (v2.4.5). Duplicate reads were removed using Picard tools (v.1.88, http://broadinstitute.github.io/picard) and BAM files were generated using Samtools (v1.2). Peak calling was performed using MACS2 (2.2.7.1) with ‘-q 0.01 --extsize 150 --keep-dup 5’ parameters to identify histone and transcription factor ChIP-seq enrichment regions over background. Robust BRD4 peaks intersected from two biological replicates were merged from both the 12A and WT treatments using Bedtools (v2.31.1).
Assembly: hg38
Supplementary files format and content: For RNA-seq, tab-delimited text file includes raw counts for each Sample. For ChIP-seq, bigWig

source_name: HeLa

growth_protocol: HeLa cell line was cultured in DMEM medium (Gibco) supplemented with 10% fetal bovine serum and 1% penicillin/ streptomycin at 37°C under 5% CO2.

extract_protocol:
For RNA-seq, Total RNA and RNA associated with different cell fractions were extracted using TRIzol™ reagent (Invitrogen, 15596026) according to the manufacturer’s protocol and quantified using NanoDrop One spectrophotometer (Invitrogen). For ChIP-seq, HeLa cells were resuspended with growth medium into a density of 106 mL-1. Crosslinking was performed by adding 1% formaldehyde (Sigma-Aldrich, F1635) into the cells followed by rotation for 20 min at RT, which was ceased by glycine with a final concentration of 0.125 M followed by rotating for 5 min at RT. Supernatants were removed and cell pellets were rinsed with ice cold PBS twice, then the cells were resuspended with lysis buffer (50 mM HEPES, pH 7.9, 5 mM MgCl2, 0.2% Triton X-100, 20% glycerol, 300 mM NaCl, 1× protease inhibitor) on ice for 10 min. Cell pellets were collected by centrifugation at 4 °C, 500 g for 5 min, then resuspended with SDS lysis buffer (50 mM HEPES, pH 7.4, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 0.1% SDS, 1× protease inhibitor) and kept on ice for 30 min. The lysates were sonicated utilizing a Bioruptor sonicator (Diagenode) at high power for 30 cycles, followed by centrifugation at 17000 × g for 10 min. Supernatants were collected into new tubes. The specified antibodies, BRD4 (E2A7X) Rabbit mAb (Cell Signaling Technology, 13440S) and HA-Tag (C29F4) Rabbit mAb (Cell Signaling Technology, 3724S), were diluted 1:50 in SDS lysis buffer and incubated with a pre-washed mixture of Protein A and G magnetic beads at 4 °C with continuous rotation for a minimum of 2 h. The beads were subsequently washed three times with SDS lysis buffer. Cell lysates were pre-cleaned with washed Protein A and G magnet beads mixture at 4 °C for 1 h with rotation, and 10% of the lysate were kept as the input control. Then, the remaining lysates and antibody-coated beads were incubated at 4 °C overnight with rotation. The beads were washed with rotation sequentially at 4 °C with the following buffers, 5 min for each: twice with SDS lysis buffer, twice with high salt wash buffer (50 mM HEPES, pH 7.5, 350 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 0.1% SDS), twice with LiCl wash buffer (10 mM Tris-HCl, pH 8.0, 250 mM LiCl, 1 mM EDTA, 0.5% NP-40, 0.5% sodium deoxycholate) and once with TE wash buffer (10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.2% Triton X-100), then eluted with 240 µL elution buffer (100 mM NaHCO3, 1% freshly added SDS) by shaking at 30 °C for 1 h. The eluent was then mixed with 14.4 µL 5 M NaCl and shaken at 65 °C for 4 h to reverse the crosslinks between proteins and DNA. Afterwards, the eluent was treated with 4 µL RNase A (Thermo Scientific, EN0531), shaken at 37 °C for 15 min, then 4 µL proteinase K (Thermo Scientific, 100005393), shaken at 65 °C overnight. The DNA was extracted by DNA clean & concentrator (Zymo Research, D4014) and set aside for subsequent qPCR assay or library preparation.
For RNA-seq, RNA-seq library preparation was performed utilizing VAHTS Universal V8 RNA-seq Library Prep Kit (Vazyme, NR605) following the manufacturer’s protocols with 1 µg initial amount of total RNA for each sample, and sequencing was performed at JMDNA Bio-Medical Technology Corporation on an Illumina NovaSeq 6000 platform. For ChIP-seq, library preparation was performed utilizing VAHTS Universal Pro DNA Library Prep Kit (Vazyme, ND608) following the manufacturer’s protocols with 10 ng initial amount of DNA for each sample, and sequencing was performed at JMDNA Bio-Medical Technology Corporation on an Illumina NovaSeq 6000 platform.

value: HeLa

value: Cervical cancer cells

value: Total, 12A


title: HeLa cells, Total RNA-seq, 12A, rep2


title: HeLa cells, Total RNA-seq, WT, rep2

value: Total, WT


title: HeLa cells, Total RNA-seq, WT, rep1


title: HUVEC, hsa-miR-34a-5p mimic, rep4

city: St. Louis

first: Rajendra

middle: S

last: Apte

state: MO

address: 660 S Euclid Ave

department: Ophthalmology

institute: Washington University School of Medicine in St. Louis

data_processing:
Basecalls and demultiplexing were performed with Illumina’s bcl2fastq software and a custom python demultiplexing program with a maximum of one mismatch in the indexing read.  RNA-seq reads were then aligned to the Ensembl release 101 primary assembly with STAR version 2.7.9a.  Gene counts were derived from the number of uniquely aligned unambiguous reads by Subread:featureCount version 2.0.3. Isoform expression of known Ensembl transcripts were quantified with Salmon version 1.5.2.  Sequencing performance was assessed for the total number of aligned reads, total number of uniquely aligned reads, and features detected.  The ribosomal fraction, known junction saturation, and read distribution over known gene models were quantified with RSeQC version 4.0.
All gene counts were then imported into the R/Bioconductor package EdgeR and TMM normalization size factors were calculated to adjust for samples for differences in library size.  Ribosomal genes and genes not expressed in the smallest group size minus one samples greater than one count-per-million were excluded from further analysis.  The TMM size factors and the matrix of counts were then imported into the R/Bioconductor package Limma. Weighted likelihoods based on the observed mean-variance relationship of every gene and sample were then calculated for all samples with the voomWithQualityWeights function and were fitted using a Limma generalized linear model with additional unknown latent effects as determined by surrogate variable analysis (SVA).  The performance of all genes was assessed with plots of the residual standard deviation of every gene to their average log-count with a robustly fitted trend line of the residuals. Differential expression analysis was then performed to analyze for differences between conditions and the results were filtered for only those genes with Benjamini-Hochberg false-discovery rate adjusted p-values less than or equal to 0.05.
For each contrast extracted with Limma, global perturbations in known Gene Ontology (GO) terms, MSigDb, and KEGG pathways were detected using the R/Bioconductor package GAGE to test for changes in expression of the reported log 2 fold-changes reported by Limma in each term versus the background log 2 fold-changes of all genes found outside the respective term.  The R/Bioconductor package heatmap3 was used to display heatmaps across groups of samples for each GO or MSigDb term with a Benjamini-Hochberg false-discovery rate adjusted p-value less than or equal to 0.05.  Perturbed KEGG pathways where the observed log 2 fold-changes of genes within the term were significantly perturbed in a single-direction versus background or in any direction compared to other genes within a given term with p-values less than or equal to 0.05 were rendered as annotated KEGG graphs with the R/Bioconductor package Pathview.
To find the most critical genes, the Limma voomWithQualityWeights transformed log 2 counts-per-million expression data was then analyzed via weighted gene correlation network analysis with the R/Bioconductor package WGCNA.  Briefly, all genes were correlated across each other by Pearson correlations and clustered by expression similarity into unsigned modules using a power threshold empirically determined from the data.  An eigengene was then created for each de novo cluster and its expression profile was then correlated across all coefficients of the model matrix.  Because these clusters of genes were created by expression profile rather than known functional similarity, the clustered modules were given the names of random colors where grey is the only module that has any pre-existing definition of containing genes that do not cluster well with others. These de-novo clustered genes were then tested for functional enrichment of known GO terms with hypergeometric tests available in the R/Bioconductor package clusterProfiler.  Significant terms with Benjamini-Hochberg adjusted p-values less than 0.05 were then collapsed by similarity into clusterProfiler category network plots to display the most significant terms for each module of hub genes in order to interpolate the function of each significant module.  The information for all clustered genes for each module were then combined with their respective statistical significance results from Limma to determine whether or not those features were also found to be significantly differentially expressed.
Assembly: Human Ensembl GRCh38.101
Supplementary files format and content: group_mt-group_mc_Differential_Expression: Differential gene expression performed comparing scrambled control mimic vs. has-miR-34a-5p mimic.
Supplementary files format and content: all.gene_counts.tsv: Raw counts.

source_name: blood vessel

growth_protocol: We used primary human umbilical vein endothelial cells (HUVECs, ATCC PCS-100-010 ). These cells were maintained in Vascular Cell Basal Media (ATCC PCS-100-030) supplemented with Endothelial Cell Growth Kit-VEGF (ATCC PCS-100-041).

extract_protocol:
After incubation, we washed cells twice with PBS, then used TRIzol (ambion) and RNeasy Plus Mini Kit (Qiagen) to extract RNA following the manufacturer's recommended protocol.
Total RNA integrity was determined using Agilent Bioanalyzer or 4200 Tapestation. Library preparation was performed with 500ng to 1ug of total RNA. Ribosomal RNA was removed by an RNase-H method using RiboErase kits (Kapa Biosystems). mRNA was then fragmented in reverse transcriptase buffer and heating to 94 degrees for 8 minutes. mRNA was reverse transcribed to yield cDNA using SuperScript III RT enzyme (Life Technologies, per manufacturer's instructions) and random hexamers. A second strand reaction was performed to yield ds-cDNA. cDNA was blunt ended, had an A base added to the 3' ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 12-15 cycles using primers incorporating unique dual index tags. Fragments were sequenced on an Illumina NovaSeq-6000 using paired end reads extending 150 bases.

treatment_protocol: We treated HUVECs with either a scrambled control mimic (QIAGEN Cat. #339173) or a hsa-miR-34a-5p mimic (QIAGEN Cat. #339173) for 48h.

value: blood vessel

value: vascular endothelial cells

value: hsa-miR-34a-5p mimic


title: HUVEC, hsa-miR-34a-5p mimic, rep3

value: primary human umbilical vein endothelial cells


title: HUVEC, hsa-miR-34a-5p mimic, rep2


title: HUVEC, scrambled control, rep4

value: scrambled control mimic


title: HUVEC, scrambled control, rep3


title: HUVEC, hsa-miR-34a-5p mimic, rep1


title: HUVEC, scrambled control, rep2


title: HUVEC, scrambled control, rep1


title: C4_2B_Ai_2_3 biol rep 6

city: Houston

first: Arun

last: Sreekumar

state: Texas

address: One Baylor Plaza

department: Molecular and Cell Biology

institute: Baylor College of Medicine

data_processing:
The fastq reads were trimmed using Cut adapt v2.3 (Martin, 2011). FastQC v0.11.8 was used to ensure the quality of data (Andrews, 2010).
Reads were mapped to the reference genome GRCh38 (ENSEMBL), using STAR v2.7.8a (Dobin et al., 2013) and assigned count estimates to genes with RSEM v1.3.3 (Li and Dewey, 2011)
Alignment options followed ENCODE standards for RNA-seq.
Assembly: GRCh38 (ENSEMBL 109)
Supplementary files format and content: tab-delimited text files include RSEM counts

source_name: C42B xenograft

extract_protocol:
RNA was extracted using the RNeasy mini kit (Qiagen, Hilden, Germany). Extracted RNA was quantified and its purity (absorbance at 260/280 nm) was verified using a spectrophotometric plate reader (Synergy HTX multi-mode reader, Biotek Instruments, Winooski, VT).
Library preparation and sequencing were done at the University of Michigan Advanced Genomics Core. Library preparation and selection of poly-adenylated transcripts were done using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, E7490) and xGen Broad-range RNA Library Prep (IDT, 1009813) with xGen Normalase UDI Primers (IDT, various).

value: C42B xenograft

value: ADA over expression


title: C4_2B_Ai_2_2 biol rep 5


title: C4_2B_Ai_2_1biol rep 4


title: C4_2B_Ai_1_2 biol rep 2


title: C4_2B_Ai_1_3 biol rep 3


title: C4_2B_Ai_1_1biol rep 1


title: C4_2B_Ai_ND_2_3 biol rep 6

value: Control


title: C4_2B_Ai_ND_2_2 biol rep 5


title: C4_2B_Ai_ND_2_1 biol rep 4


title: C4_2B_Ai_ND_1_1 biol rep 1


title: C4_2B_Ai_ND_1_3 biol rep 3


title: C4_2B_Ai_ND_1_2 biol rep 2


title: C4_2B_Ci_2_3 biol rep 6


title: C4_2B_Ci_2_2 biol rep 5


title: C4_2B_Ci_2_1 biol rep 4


title: C4_2B_Ci_1_3 biol rep 3


title: C4_2B_Ci_1_1 biol rep 1


title: C4_2B_Ci_1_2 biol rep 2


title: 22Rv1_Ai_2_3 biol rep 6

source_name: 22Rv1 xenograft

value: 22Rv1 xenograft


title: 22Rv1_Ai_2_1 biol rep 4


title: 22Rv1_Ai_1_3 biol rep 3


title: 22Rv1_Ai_2_2 biol rep 5


title: 22Rv1_Ai_1_1 biol rep 1


title: 22Rv1_Ai_1_2 biolrep 2


title: 22Rv1_Ai_ND_1_3 biol rep 5


title: 22Rv1_Ai_ND_1_1 biol rep 3


title: 22Rv1_Ai_ND_2_3 biol rep 2


title: 22Rv1_Ai_ND_2_1 biol rep 1


title: 22Rv1_Ci1_3 biol rep 6


title: 22Rv1_Ai_ND_1_2 biol rep 4


title: 22Rv1_Ci_2_3 biol rep 5


title: 22Rv1_Ci_2_2 biol rep 4


title: 22Rv1_Ci_2_1 biol rep 3


title: 22Rv1_Ci_1_2 biol rep 2


title: 22Rv1_Ci_1_1 biol rep 1


title: CCNB1IP1rep3

city: Kunming

first: lei

last: shi

state: Yunnan

address: No.17 Longxin Road

institute: Kunming Institute of Zoology

data_processing:
Illumina  software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to GRCm39primary(https://www.gencodegenes.org/)whole genome using Hisat2
Fragments per kilobase of transcript per million mapped fragments (FPKM) were calculated. First step, align RNA seq reads to the genome by using  Hista2 ; and then Cufflinks2 will assemble transcripts and computed the FPKM vlues(http://cufflinks.cbcb.umd.edu/),following protocol from Pertea, M.et al  Nat Protoc 11, 1650-1667, (2016).
Assembly: GRCm39primary
Supplementary files format and content: tab-delimited text files include FPKM values for each Sample

extract_protocol:
Total RNA was extracted from the centrifuged cells by using RNeasy Mini Kit (QIAGEN, #74104)
RNA sequencing library construction followed VAHTs mRNA-seq V3 Library Prep Kit for Illumina (Vazyme, #NR611). The total RNA input was 0.7-1ug, mRNA (with ploy-A) binds to mRNA capture beads (with Oligo dT), mRNA is eluted from beads and then fragmented, synthesis of the 1st strand of cDNA using random primers, synthesis of the 2nd strand of cDNA after RNase H digestion. Size selection and library amplication after end repair and adapter ligation, the final RNA sequencing library length is between 320-420bp. Library quality determination analyzed by an Agilent Technologies 2100 Bioanalyzer.

value: neuroblastoma

value: transfected with pCS2-eGFP-CCNB1IP1


title: CCNB1IP1rep2


title: GFPrep3

value: transfected with pCS2-eGFP-N


title: CCNB1IP1rep1


title: GFPrep2


title: Circulating cells of colony stage B2

city: Exeter

first: Marie

last: Lebel

address: Stocker Road

department: Biosciences

country: United Kingdom

institute: University of Exeter

description:
Library name: Sample 10
10X Genomics, live cells, raw matrix

data_processing:
Individual 10X sample libraries were demultiplexed using Cell Ranger Makefastq v7.0.0 with default settings.
Reads were quality-checked using FASTQC. For the buds samples (samples 1-8): Reads were mapped with CellRanger count (v7.1.0). The archived processed files were filtered for empty droplets. using CellRanger count automatic filtering. For the circulating cells samples (sample 9-10): Reads were mapped with CellRanger count (v6.1.2). The archived processed files are not filtered for empty droplets.
Assembly: The reference genome was prepared by concatenating: (1) the nuclear genome (BioProject PRJNA1225683), with the addition of a manually annotated Piwi2 gene (Boschl.Bs14.g18242-nested) to the GTF and (2) the published mitochondrial genome (sc6a-b isolate, GenBank: HF548551.1, GTF filtered to only the protein coding genes).
Supplementary files format and content: For the buds samples (samples 1-8):  Tab-separated files and matrixes.
Supplementary files format and content: For the circulating cells samples (sample 9-10): H5 files.

organism: Botryllus schlosseri

source_name: Circulating cells from vasculature

extract_protocol:
For the buds samples (samples 1-8): buds were microdissected along with their surrounding tunic and some ampullae and washed twice in in calcium- and magnesium-free artificial sea water (CMFASW; 31g/L NaCl, 0.8g/L KCl, 0.29g/L NaHCO3, 1.6g/L Na2SO4, pH 8.0) with 40U/ml RNAsin Plus (Promega). Buds were then detunicated and dissociated by pipetting in 0.2µm-filtered 1% Trypsin (m./v.) in CMFASW with 40 U/ml RNAsin Plus for 20-30 min. Cell suspensions with over 85% cell viability were fixed in ACME (10% acetic acid, 15% methanol, 10% glycerol in molecular grade water) with 40U/ml RNAsin Plus for 1h on ice, gently shaking, then stored at -20°C for up to a few days. Fixative was removed from cell suspensions by washing and centrifugating cells in 0.2µm-filtered 1% BSA in PBS, then stored at -80°C with 10% (v./v.)  DMSO. On the day of library preparation, cryopreserved samples were slowly thawed on ice, centrifuged and  resuspended in 1% BSA-PBS with 40U/ml RNAsin Plus. They were then filtered with 40µm cell strainer to remove largest aggregates, stained with Draq5 and sorted by FACS to select only single cells. For the circulating cells samples (sample 9-10): colonies were cleaned a couple hours before dissections and kept in 0.22µm filtered ASW until blood collection. At collection time, colonies were dried gently with a Kim wipe, then 100-200 µL of PBS-M-DTT (0.7M D-mannitol in 1X PBS with 0.01 M dithiothreitol, added fresh each day) was added on top. Blood was extracted by making multiple cuts in vascularized regions of the colony, and gently pressing on it. The PBS-M-DTT with suspended blood cells was collected and kept in a microcentrifuge tube on ice. Blood cells from several colonies at the same stage were pooled. Each cell suspension was filtered through a 40µm strainer into a microcentrifuge tube which had been coated with 10% (v./v.) bovine serum albumin in Dulbecco’s phosphate-buffered saline and washed by centrifugation before resuspension in PBS-M-DTT. The cell viability prior to encapsulation was 79% (Sample 9 -A1) and 80% (Sample 10 - B2).
Cell encapsulation, RT, cDNA amplification, and library preparation were carried out using the Chromium Next GEM Single Cell 3′ v3.1 Kits (10X Genomics 1000123, 1000127, and 1000190). For the buds samples (samples 1-8): The manufacturer’s guidelines were followed. The libraries were then sequenced (paired-end) on an Illumina NextSeq2000. When necessary, resequencing was performed to achieve a sequencing saturation of approximately 40% or higher. FASTQ files in this archive contain the concatenated reads from all sequencing of the same library. For the circulating cells samples (sample 9-10): The manufacturer’s guidelines were followed except for one step. To prepare the RT mix prior to cell encapsulation, the manufacturer protocol instructs to add nuclease-free water, then to add the cell suspension; we added PBS-M instead of nuclease-free water to adjust for live cells osmolarity.

value: Circulating cells from vasculature

tag: developmental stage

value: colony stage B2

value: WT, 6 clones pooled


title: Circulating cells of colony stage A1

description:
Library name: Sample 9
10X Genomics, live cells, raw matrix

value: colony stage A1

value: WT, 5 clones pooled