title: Fox Chase Cancer Center Homo sapiens moNIA15k
status: Public on Oct 31 2006
contact.city: Philadelphia
contact.first: Xiangxi
contact.last: Xu
contact.email: Xiangxi.Xu@fccc.edu
contact.state: PA
contact.address: 333 Cottman Ave
contact.department: Ovarian Cancer and Tumor Cell Biology
contact.country: USA
contact.web_link: http://www.fccc.edu
contact.institute: Fox Chase Cancer Center
organism: Mus musculus
manufacture_protocol: Targets:
  	cDNA (50-200ng/µl in 50% DMSO) 10µl
  or
  	Oligo (20-100 µM in 50% DMSO) 10 µl
  	Targets are stored in a dessicator at –80 ºC after printing
  Slides:
  Home made Poly-L-Lysine coated slides should be aged for at least 3 weeks before printing. Coated slide can be stored at RT for at least 6 months.
  Arrayer
  OmniGrid (GeneMachines) with MicroSpotting pins (Telechem)
  Printing Configuration
  	Pin			4 x 4
  	Spot-spot spacing	240 µm
  	Spot size		150-200 µm
  	Subgrid		18 x 18 spots
  	Grid			12 x 4
  	Humidity		45%
  	Temperature		20-25 ºC
  Post-Processing of Arrays
  Following printing, the slides are allowed to dry and spotted DNA is bound to slide by baking slides at 80 ºC for 2 hours. Then printed slides are blocked with succinic anhydride (Brown protocol).
  Arrays are used immediately or stored in dessicator at RT for at least 6 months.