title: Listeria monocytogenes strain F2365 5.6K
status: Public on Dec 31 2008
contact.city: Wyndmoor
contact.first: yanhong
contact.last: Liu
contact.email: yanhong.liu@ars.usda.gov
contact.state: PA
contact.address: 600 E. Mermaid Lane
contact.country: USA
contact.institute: ERRS-ARS-USDA
contact.phone: 215-233-6587
organism: Listeria monocytogenes
manufacture_protocol: The custom oligonucleotide microarray is synthesized on a semiconductor using an electrochemical synthesis process. Each oligonucleotide probe (spot) is synthesized on a platinum electrode that is independently controlled by the synthesizer’s computer. Synthesis is based on established phosphoramidite chemistry and occurs at thousands of sites simultaneously according to a computer algorithm that activates only specified electrodes. Since physical photolithographic masks, mechanical micromirrors, or inkjet technologies are not involved in the process, all probes can be easily changed without extra time or cost.
  The oligonucleotides are synthesized in a proprietary porous reaction layer that coats the surface of the semiconductor, and acts to covalently attach the oligonucleotide to the surface of the chip.