title: Avian Innate Immunity Microarray (AIIM)
status: Public on Sep 24 2004
contact.city: Newark
contact.first: Calvin
contact.last: Keeler
contact.email: ckeeler@udel.edu
contact.state: DE
contact.department: Animal and Food Sciences
contact.country: USA
contact.web_link: http://www.aviangenomics.udel.edu
contact.institute: University of Delaware
contact.phone: 3028312524
organism: Gallus gallus
description: This microarray was created using all of the unique elements in 4 individual EST libraries created using various sources of macrophages. It was further supplemented with RT-PCR amplified genes of interest and clones from Peter Kaiser. Three libraries were made from peripheral blood derived macrophages: Unstimulated (Control), LPS stimulated for 2-4 hours (LPS), and INF-gamma stimulated for 2-4 hours (IFN).  The fourth library was created from HD11 cell line macrophages stimulated for 0-72 hours (pmp1c).  134 additional clones were added from clones in the University of Delaware ChickEST library collections.  In addition, 13 full-length cDNA clones were kindly provided by Dr. Pete Kaiser. Finally, 40 elements were amplified directly by RT-PCR from targeted genes of interest identified on the avian genome.  Total, this array contains 4,959 unique elements.
  Keywords = Avian
  Keywords = innate immunity
  Keywords = macrophage
  Keywords = AIIM