title: Xylella fastidiosa 9a5c Biochip v.2.0
status: Public on Dec 21 2006
contact.city: Santo André
contact.first: Luiz
contact.middle: R.
contact.last: Nunes
contact.email: luiz.nunes@ufabc.edu.br
contact.state: SP
contact.address: Rua Santa Adélia 166
contact.department: Centro de Ciências Naturais e Humanas
contact.country: Brazil
contact.institute: UFABC - Universidade Federal do ABC
contact.phone: (55) (11) 4798-7104
organism: Xylella fastidiosa
description: Xylella fastidiosa, isolate 9a5c
manufacture_protocol: The array consists of DNA amplicons from ~2300 ORFs from the genome of the phytobacterium Xylella fastidiosa 9a5c. The amplicons were PCR amplified with specifically designed primer pairs and purified with the QiaQuick 96 PCR purification system (Qiagen), dried in a Savant speed-vac, resuspended in 50 % DMSO at a final concentration of 100-200 mg/ml and spotted onto CMT-GAPS Coated Slides (25 x 75 mm, Corning) using an Affimetrix 427 arrayer. Those interested in obtaining the X. fastidiosa microarrays, please contact lnunes@umc.br or reginaco@umc.br for further details.