title: BC013_nirS
status: Public on May 02 2017
contact.city: Gulf Breeze
contact.first: Jessica
contact.middle: Anne
contact.last: Lisa
contact.email: jlisa610@gmail.com
contact.state: FL
contact.address: 1 Sabine Island Drive
contact.department: Office of Research and Development
contact.country: USA
contact.institute: U.S. Environmental Protection Agency
contact.phone: 1-850-934-92177
organism: uncultured bacterium
description: The probes on this array are 90-mers that consist of the 70-mer nirS sequence and a 20-mer universal reference sequence.
manufacture_protocol: 1.The probe oligonucleotides are printed onto Corning Ultragaps or Erie Scientific Epoxy Silane slides using a DeRisi style arrayer (DiRisi et al., 1997) and pins from Parallel Synthesis.
  2.The 70-mer oligo probes (Operon Technologies) were adjusted to a concentration of 0.05 mg ml-1 in 50%  DMSO and were spotted on CMT-GAPS amino silane-coated  glass slides (Corning, Corning, NY).
  3. After printing, the slides were baked at 80°C for 3 h and stored in the dark at room temperature under slight vacuum in a dessicator.