title: Fluidigm Human adipose tissue markers analysis
status: Public on Nov 27 2014
contact.city: Toulouse
contact.first: Nathalie
contact.last: Viguerie
contact.address: Avenue Poulhes
contact.department: I2MC U1297
contact.country: France
contact.institute: INSERM
organism: Homo sapiens
description: Fluidigm PCR assay
manufacture_protocol: The 222 transcripts were selected from previous published and unpublished Agilent Human 4x44k microarray analyses of human subcutaneous adipose tissue biopsies performed on limited number of individuals as described in Viguerie N et al (Plos Genetics 2012, 8: e1002959). These genes encode proteins involved in various pathways including metabolism (43% of the transcripts), immune response (20%), cell differentiation and proliferation (9%), cell and tissue structure (7%), transport (5%), signal transduction (3%) and response to stress (1.3%). The list of genes extracted from the previous microarray data analysis includes 89 genes previously shown as markers of subcutaneous adipose tissue from obese insulin resistant individuals with metabolic syndrome, 44 genes described as markers of subcutaneous adipose tissue from lean individuals, 39 markers of weight changes after caloric restriction, 40 genes selected from previous caloric restriction induced weight loss studies, and 18 unpublished predictors of weight change to distinguish between those individuals that will regain weight after calorie restriction.