title: NCI NimbleGen Homo sapiens HG19 Whole Genome 385K Tiling Set version 1
status: Public on Oct 24 2011
contact.city: Princeton
contact.first: Sudhir
contact.last: Varma
contact.email: sudhirv4rma@gmail.com
contact.state: NJ
contact.address: 1215 Wessex Pl
contact.country: USA
contact.institute: HiThru Analytics
organism: Homo sapiens
description: 385K element tiling array based on NCBI Build 35 of the human genome (HG17) and re-mapped to NCBI Build 35 (HG19); 50-mer tiling with a median probe spacing of 6,000 bp. Positional information for re-mapped probes available in included file 2005_03_16_HG19_WG_CGH.pos
manufacture_protocol: Tiling-path CGH arrays were designed for whole-genome analysis with a single array minimum probe spacing of 5 Kb, and a median probe spacing of 6 Kb.  HG17 (NCBI 35) sequence was repeat-masked using Repeat-Masker (20).  Unique probes were selected as described by Selzer et al. (Pubmed ID: 16075461). Instead of being of fixed length, oligonucleotides were of variable length, adjusted to match a melting temperature of 76°C and constrained to a minimum of 45 bp and maximum of 85 bp in length.  The arrays were constructed by maskless array synthesis technology, with ~385,000 oligonucleotides being synthesized by photolithography on an array.