title: Broad Institute Human L1000 epsilon
status: Public on Jul 15 2015
contact.city: Cambridge
contact.first: Aravind
contact.last: Subramanian
contact.state: MA
contact.address: 415 Main Street
contact.country: USA
contact.institute: The Broad Institute
organism: Homo sapiens
description: L1000 is a high-throughput gene-expression assay based on the direct measurement of a reduced representation of the transcriptome and computational inference of the portion of the transcriptome not explicitly measured. The epsilon version of the assay measures 978 Landmark genes and 80 control genes. These direct measurements are combined with a statistical model to infer additional mRNA expression levels.
manufacture_protocol: L1000 is a high-throughput gene-expression assay that combines locus-specific ligation-mediated amplification with an optically addressed microsphere and flow cytometric detection system from Luminex Inc. to measure selected mRNA transcripts.