title: Operon Mouse 36k v4.0
status: Public on Sep 01 2008
contact.city: Stanford
contact.first: Daniel
contact.last: Haag
contact.email: haag@stanford.edu
contact.state: Santa Clara
contact.address: 265 Campus Drive
contact.department: ISCBRM
contact.country: USA
contact.institute: Stanford School of Medicine
organism: Mus musculus
description: The array format contains of 36480 spots in 48 subarrays each of which is build of 28 columns, 27 whole rows and 4 spots within a 28th row. This number includes empty buffer spots, sporadic replicates of single oligos and control sequences. Each subarray is printed by one spotting pin. The distance between spot centers is about 155µm. Spot diameter is approximately 100µm.
manufacture_protocol: Lyophilized 70mer oligonucleotides in 384well plates obtained from Operon are dissolved in 3x SSC, 1,5 M Betaine at a concentration of 20µM (35852 probes from Mouse Genome Oligo Set Version 4.0). Oligos are spotted on epoxysilane coated glass slides using 48 Micro Spotting Pins (946MP3, ArrayIt) in a Versarray Chipwriter Pro System (BioRad). Spotted Chips are baked for 60 minutes at 70°C and stored airtight under dry condition at 4°C. Before hybridization processing slides containing spotted arrays are blocked with 0,3% ethanolamine, 0,1% SDS in 0,1M Tris (pH 9,0).