title: Hebrew University of Jerusalem miRNA spotted array
status: Public on Nov 24 2009
contact.city: Jerusalem
contact.first: Hermona
contact.last: Soreq
contact.email: soreq@cc.huji.ac.il
contact.address: Safra Campus, Givat Ram
contact.department: Department of Biological Chemistry
contact.country: Israel
contact.institute: The Hebrew University of Jerusalem
organism: Homo sapiens
  Mus musculus
manufacture_protocol: The mirVana oligo set (Ambion, Austin, TX; Catalog number 1564V1) was used to construct an in-house array with >200 spotted probes complementary to known human and mouse miRs. The mirVana probeset was dissolved in 3XSSC to a final concentration of 20 mM, and each oligo printed on Ultragaps slides (Corning, Corning, NY) 6 or more times, using the MicroGrid spotter (Genomic Solutions, Holliston, MA).