title: Intel array v1
status: Public on Dec 02 2011
contact.city: Oberlin
contact.first: Jordan
contact.last: Price
contact.state: OH
contact.address: 119 Woodland St
contact.country: USA
contact.institute: Oberlin College
organism: Homo sapiens
description: Photolithographic synthesis of peptides on silicon substrate
manufacture_protocol: Wafer derivatization and linker attachment. We derivatized blank six-inch silicon wafers (Silicon Valley Microelectronics, Santa Clara, CA) by thermal oxidation followed by salination via immersion in a 0.5% v/v mixture of 3-aminopropyltriethoxysilane (Sigma-Aldrich, St. Louis, MO) in 95% ethyl alcohol (EtOH, Sigma) for 30 min at RT. We then cured wafers at 100 °C for 1 h under ultrapure and ultradry N2 (Airgas, Sacramento, CA) to crosslink silane groups to each other and to the silicon surface. Following salination, we added a PEG6 linker to the wafer surface to facilitate addition of custom peptides.