title: DGRC-Drosophila melanogaster-DGRC1-15552 15k cDNA array_v5
status: Public on Jun 24 2010
contact.city: Guangzhou
contact.first: TIAN
contact.last: TANG
contact.email: ttian_8@hotmail.com
contact.address: Xingangxi Rd. #135
contact.country: China
contact.institute: Sun Yat-Sen University
organism: Drosophila melanogaster
manufacture_protocol: DGRC-1 amplicon transcriptome microarrays are spotted with DNA fragments amplified from genomic DNA of the Oregon R strain of Drosophila melanogaster using gene-specific oligonucleotide primer pairs made by Incyte Genomics. The primers were designed against release 1 of the annotated genome sequence and were selected to amplify fragments which have minimal sequence similarity to other genes, size range between 100-600 bp, and location biased towards the 3'-end of transcripts. The array also contains the following controls: a ubiquitously expressed gene (actin 5C), a spotting buffer-only control (3XSSC plus 1.5M Betaine), exogenous(non-Drosophila) probes (7 from Arabidopsis and 8 Ambion array control PCR spots from bacteria that can be used with spiking RNAs), and three exogenous markers: GAL4, lacZ, and FLP. The DGRC arrays are printed using an Omnigrid 300 contact-printing instrument and 48 silicon split-pins on Corning GAPS II slides.