title: NCI Mus musculus 10K FCRF UniGEM2
status: Public on May 17 2006
contact.city: Genoa
contact.first: Paolo
contact.last: Fardin
contact.email: paolofardin@ospedale-gaslini.ge.it
contact.address: Largo G. Gaslini 5
contact.department: Molecular Biology
contact.country: Italy
contact.institute: Istiuto Giannina Gaslini
organism: Mus musculus
manufacture_protocol: The custom arrays are manufactured by the NCI Frederick Laboratory of Molecular Technology Microarray Facility (NIH/NCI/SAIC/LMT). Microarrays are printed with BioRobotics TAS II robotic arrayer from Genomic Solutions that is fitted with a printhead containing 32 SMP3 printing pins from Telechem International. The mouse cDNA probe set is based on 9984 PCR products from Incyte's mouse UniGEM 2.0 library. The set includes 9234 Unique GenBank Accessions; 8882 mapped into Mm UniGene Build #1201; 8656 Unique UniGene Clusters; 3463 EST clusters; 5193 Named clusters. The cDNA probes are distributed into 384-well polypropylene plates (Genetix, 384 micro array) and dissolved in 3XSSC print buffer at a concentration of 50-100 ug/ml. The microarray spot densities are 235 microns (center-to-center), and one spot is printed per probe per slide. The microarrays are printed on Fisher Premium microscope slides (cat# 12-544-1) coated with poly-L-lysine and post-processed according to the procedures on the mAdb NCI website.