title: Paramecium bursaria Chlorella Virus-1 (PBCV-1) oligo array, v.1.2
status: Public on Jan 10 2010
contact.city: Lincoln
contact.first: James
contact.middle: L
contact.last: Van Etten
contact.email: jvanetten@unlnotes.unl.edu
contact.state: NE
contact.address: 4240 Fair Street
contact.department: Plant Pathology
contact.country: USA
contact.institute: University of Nebraska-Lincoln
contact.phone: 402-472-3168
organism: Paramecium bursaria Chlorella virus 1
description: This chip  (v.1.2) is similar to the v.1.0 used for the PBCV-1 time course analysis, however, because of a problem during probe printing a few spots were out of place. This problem forced us to create a new annotation file. Thus, we decided to generate a new platform for it to avoid confusion. Chip v.1.2 was mainly used to the aphidicolin treatment experiments.
  The Platform data table reflects a condensed representation of the array's replicate features.
manufacture_protocol: 50-mer probes representing each major PBCV-1 protein encoding gene (CDS) were designed and synthesized by MWG Biotech (Ebersberg, Germany) (20-80% GC, 60-80º C melting temperature).  Probes were spotted onto CMT-GAPS silane-coated slides (Corning, Lowell, MA) using Omnigrid 100 (Genomic Solutions, Ann Arbor, MI) according to manufacturer’s instructions.  Probes were printed in quadruplicates on every slide.