title: UMich_Proteus mirabilis_v1.0 3.7k array
status: Public on Jun 07 2010
contact.city: Ann Arbor
contact.first: Melanie
contact.middle: M
contact.last: Pearson
contact.email: mpears@umich.edu
contact.state: MI
contact.address: 1150 W Medical Center Dr
contact.department: Microbiology and Immunology
contact.country: USA
contact.institute: University of Michigan Medical School
organism: Proteus mirabilis HI4320
manufacture_protocol: 70mer oligos corresponding to 3719 predicted ORFs in the Proteus mirabilis HI4320 genome were designed by Operon Biotechnologies, Inc.  The average GC% of all gene sequences was 39.618% and the Tm corresponding to this GC% was 74.0 C.  Test runnings suggest that this is optimal Tm for this species.  Out of 3719 sequences, 3714 have the oligos that meet the design criteria.  Oligos were suspended in NaPO4 buffer and spotted in triplicate onto Corning UltraGAPS slides by Microarrays, Inc.