title: NCSU_Glycine max_5.7K
status: Public on Sep 07 2007
contact.city: Tallahassee
contact.first: Andre
contact.middle: Southernman Teixeira
contact.last: Irsigler
contact.email: irsigler@bio.fsu.edu
contact.state: FL
contact.address: Chieftan Way
contact.department: Biology
contact.country: USA
contact.institute: Florida State University
organism: Glycine max
manufacture_protocol: The cloned cDNA fragments from soybean libraries were amplified with M13 primers, purified using PCR Cleanup Filter Plates (Millipore, Bedford, MA), and eluted in water (according to the manufacturer’s protocol). An aliquot of each amplified fragment reaction was separated through a 1% (w/v) agarose gel and visualized with ethidium bromide to assess size, quality and quantity. The purified PCR products were transferred to 384-well plates, and diluted with an equal volume of DMSO (Sigma). Finally, the PCR products were arrayed onto UltraGAPS slides (Corning, Corning, NY) using a 417TM Arrayer (Affymetrix, Santa Clara, CA), cross-linked by exposure to UV light at 250 mJ and baked at 75°C for 2 h