title: Ocimum Biosolutions Zebrafish 14K OciChip
status: Public on Feb 24 2010
contact.city: Hyderabad
contact.first: Ocimum
contact.last: Biosolutions
contact.email: govind.a@ocimumbio.com, sandhya.p@ocimumbio.com, hazira.b@ocimumbio.com, anwaruddin.m@ocimumbio.com
contact.state: Andhra Pradesh
contact.address: Banjara Hills Road No., 1 VI Floor Reliance Classic
contact.department: Bioinformatics
contact.country: India
contact.institute: Ocimum Biosolutions
organism: Danio rerio
manufacture_protocol: Array Design and Printing
  Zebrafish 14K Ocichip consists of 14240 spots printed on Corning® epoxy coated  glass slides using Omnigrid (Gene Machines). Chip has been printed using 4x8 SMP3 pins on 75.5x 25.5x1.3 mm microarray glass slides. The origin offset values were 0 for X direction and 10000 for Y direction. The dot to dot distance is 190 mm; distance between subarrays is 150 mm and no of dots per array is 21x22=462. Oligos were printed using spotting buffer A from Ocimum Biosolution at 50% humidity and temperature at 22°C. The time taken by the robotic gridder to print Zebrafish14K altogether was 28 hrs. Each probe was checked for its performance by standard QC process followed at Ocimum Biosolutions. Printed slides were stored in a light-tight box in a bench-top dessicator, with desiccant and were kept at 4°C until they were used for hybridization