title: German Cancer Research Center Operon Oligonucleotide 25K Array
status: Public on Jul 09 2010
contact.city: Heidelberg
contact.first: Stefan
contact.middle: M
contact.last: Pfister
contact.email: s.pfister@dkfz.de
contact.address: INF 580
contact.department: Molecular Genetics
contact.country: Germany
contact.web_link: http://www.dkfz.de/en/genetics/staff/staff_detail/s_pfister.html
contact.institute: German Cancer Research Center
contact.phone: +49 6221 424593
organism: Homo sapiens
manufacture_protocol: Spotted long oligonucleotide array produced using the Operon Human Genome Oligo Set Version 2.1 (21,329 70mer oligonucleotides, based on build #147 of the Human UniGene database). Keywords = spotted Keywords = human Keywords = long oligonucleotide Keywords = oligo Keywords = 70mer Keywords = Operon Protocol: 70mer oligonucleotides were diluted at 40 µM in FBNC buffer (G. Wrobel et al., Nucleic Acids Res 31 e67, 2003) and spotted as array duplicates using epoxy-silane coated glass slides (Schott Nexterion) and stainless steel split pins (SMP3, Telechem) at 20°C and 40% rH. Slides were dried (60' @ 60°C) and DNA was UV-crosslinked with 240 mJ/cm2 @ 254 nm. Slides were stored, sealed airtight and dry, at 4°C.