title: USP Blastocladiella emersonii 4.6 K Blasto3773 v2
status: Public on Jun 22 2007
contact.city: São Paulo
contact.first: Raphaela
contact.middle: Castro
contact.last: Georg
contact.state: São Paulo
contact.address: Av. Prof Lineu Prestes, 748
contact.department: Bioquímica
contact.country: Brazil
contact.institute: Universidade de São Paulo
organism: Blastocladiella emersonii
manufacture_protocol: The array consists of cDNA inserts from representatives ESTs from 3,773 expressed genes that were obtained through Georg and Gomes, 2007 and  Ribichich et al (2005) work. These inserts were PCR amplified with the T7 forward and SP6 reverse primers, and the amplified products were purified with a Millipore Multiscreen Filtration Plate (MAFB NOB), eluted with Tris-HCl 10 mM, pH 8.0 to a final concentration of 100 to 200 ng/μl and spotted onto Star 7 slides (Amersham Biosciences) using a Generation III Microarray SpotterTM (Amersham Biosciences).