title: UHN Yeast 6.4kv6
status: Public on Dec 22 2007
contact.city: Rehovot
contact.first: Naama
contact.last: Barkai
contact.email: naama.barkai@weizmann.ac.il, simchen@vms.huji.ac.il
contact.address: P.O box 26
contact.department: Molecular Genetics
contact.country: Israel
contact.institute: The Weizmann Institute of Science
organism: Saccharomyces cerevisiae
description: Grid rows-12; Grid columns-4; Rows-20; Columns-20; Grid to grid distance-4500um; Spot to spot distance-200um; Spot size-100um.
manufacture_protocol: 1. Acquisition of bacterial clone set: our Yeast 6.4k clone set is derived from Research Genetics Genepairs, Detailed information about the ORFs and sequences can be found at http://www.yeastgenome.org/  2. Clone set is PCR amplified and DNA purified in working 96 well plates. 3. The purified DNA is then transferred to 384 well source plates with DNA concentration ranging from .10ug/ul to 0.25ug/ul.  These are used for spotting. We use high precision robots that can use up to 48 pins or print heads.   The Y6.4kv6 slides are printed using 48 pins and we use UltraGAPS ™ slides (Corning Inc.).  We are able to print up to 120 slides in each batch and batches that result in greater than 95% printing fidelity are selected for post printing quality control tests.