title: NanoString CosMx SMI (Mus musculus)
status: Public on Sep 24 2025
contact.city: Bethesda
contact.first: GEO
contact.last: admin
contact.email: geo@ncbi.nlm.nih.gov
contact.state: MD
contact.address: 9000 Rockville Pike
contact.country: USA
contact.institute: NCBI/NLM/NIH
organism: Mus musculus
description: The methodology is described at He, S., Bhatt, R., Brown, C. et al. High-plex imaging of RNA and proteins at subcellular resolution in fixed tissue by spatial molecular imaging. Nat Biotechnol 40, 1794–1806 (2022) (https://doi.org/10.1038/s41587-022-01483-z) . Briefly, the Spatial Molecular Imager utilizes standard sample preparation methods typical for FISH or IHC on FFPE tissue sections, with the introduction of fluorescent bead-based fiducials that are fixed to the tissue to provide an optical reference for cyclic image acquisition and registration.
  CosMx SMI is a high-plex in situ analysis platform to provide spatial multiomics with formalin-fixed paraffin-embedded (FFPE) and fresh frozen (FF) tissue samples at cellular and subcellular resolution. CosMx SMI is an integrated system with mature cyclic fluorescent in situ hybridization (FISH) chemistry, high-resolution imaging readout, interactive data analysis and visualization software.
manufacture_protocol: See manufacturer's website.