title: Agilent-030447 UNMBIOCETI Biomphalaria glabrata 30K v1.0
status: Public on Jul 30 2016
contact.city: Albuquerque
contact.first: Coen
contact.middle: M
contact.last: Adema
contact.email: coenadem@unm.edu
contact.state: NM
contact.address: 1 University of New Mexico
contact.department: CETI Biology MSC03 2020
contact.country: USA
contact.institute: University of New Mexico
contact.phone: 505 277 2743
organism: Schistosoma mansoni
  Biomphalaria glabrata
  Echinostoma paraensei
description: Please note, this single spot/feature design (AMADID 030447) was superceded with a duplicate spot/feature design, using the same features.  The updated array design is AMADID 033677. For access to this platform, contact coenadem@unm.edu for collaborator status needed to purchase microarray from Agilent
  Biomphalaria glabrata (fresh water planorbid snail), a small number of features derives from trematode parasites Schistosoma mansoni and Echinostoma paraensei
manufacture_protocol: Publicly available cDNA sequences (2010) for Biomphalaria glabrata were assembled using Mira. Transcripts were analyzed and as needed reverse complemented for longest continuous ORF. A total  of 30593 predicted transcripts, supplemented with 15 mitochondrial gene sequences from Biomphalaria glabrata, 26 (12 nuclear + 14 mitochondrial) gene sequences from Schistosoma mansoni and  12 (9 nuclear + 3 mitochondrial) gene sequences from Echinostoma paraensei) were used as targets for e-array (Agilent) design of unique 60-mer probe sequences. Single replicate probes were arranged in 8 x 60k custom microarray format with Agilent 60-mer SurePrint technology.  Design name Biomphalaria glabrata Transcriptomev1_28Sep10. Created date 28 Sep 2010. Feature layout: randomized. Design ID:030447.