title: ICGEB_Homo sapiens_35K_operon oligo_ver4.0
status: Public on Dec 01 2010
contact.city: New Delhi
contact.first: Shahid
contact.last: Jameel
contact.state: Delhi
contact.address: Aruna Asaf Ali Marg
contact.country: India
contact.web_link: http://www.icgeb.org/mammalian-biology-virology.html
contact.institute: The International Centre for Genetic Engineering and Biotechnology (ICGEB)
organism: Homo sapiens
manufacture_protocol: Oligos are arrayed in Greiner 384-well flat-bottom plates. Each well contains 600 pmol of 70-mer oligo.
  Before printing, add 40 µL of 50% DMSO to each well.
  Seal plates with Corning seals.
  Incubate at 37°C for 30 minutes to aid resuspension of DNA.
  Shake plates near maximum rotational speed on flat-bed shaker for 1 minute.
  Centrifuge plates at 2000 rpm for 3 minutes.
  Remove seals and cover with plate lids. Place in appropriate location of plate cassette.
  Printed with 48 SMP3 pins on aminosilane slides under 50% RH and 25 degree centigrade