title: INSERM Rat Operon Pronto plus Systems 6 Oligoarray
status: Public on Jul 05 2006
contact.city: Toulouse
contact.first: Philippe
contact.middle: V
contact.last: Rouet
contact.email: Philippe.rouet@inserm.fr
contact.state: BP84225
contact.address: 1 Ave Jean Poulhes
contact.department: Cardiology
contact.country: France
contact.institute: INSERM U1048
contact.phone: 00 33 5 31 22 41 19
organism: Rattus norvegicus
description: Deposition on each glass slide of the cDNA from one lean with one obese SHHF rats
manufacture_protocol: Rat oligos (set V3 from OPERON, 73 plates/96 wells) were loaded at 300 pmol/wells. Oligos were resuspended in 20µl 3X SSC buffer (concentration finale 20µM), plates were shaken  and frozen before spotting. Pirot to spotting, plates are defrozen one hour at room temperature and centrifuged. Spotting was performed as single spot on Ultragaps glass slides (CORNING) using Qarray mini robot (GENETIX). Atmosphere moisture was set at 50% and plates were cooled to 15°C to prevent drying. Spotting design was as follow:
  48 pins therefore 48 grids
  each grid contains 24x25 spots