title: AGRF Human 8K cDNA Microarray (muscle study)
status: Public on Oct 24 2007
contact.city: Clayton
contact.first: Nigel
contact.middle: Keith
contact.last: Stepto
contact.email: nigel.stepto@med.monash.edu.au
contact.state: VIC
contact.address: Wellington Road
contact.department: Physiology
contact.country: Australia
contact.institute: Monash University
organism: Homo sapiens
manufacture_protocol: Array information:
  H8K020304
  Spotted
  Corning GAPS-II Amino silane Slides
  25x75mm Slides
  Corning GAPS-II Amino silane Slides
  16128 dots
  Reporter Type:
  PCR products of cDNA clones
  Double  stranded
  Primer  sequence used to amplify human 8K clone set plates 1-75
  Reverse 5'gtgagcggataacaatttcacacaggaaacagc3'
  Forward 5'ctgcaaggcgattaagttgggtaac 3'
  Primer  sequence used to amplify human 8K clone set plates 76-78
  Reverse 5'cacaggaaacagctatgacca3'
  Forward 5'acggccagtgccagcttat3'
  cDNA Clone set from Research Genetics, Alabama, USA
  Human 8K clone set obtained Nov 2001, Housed at AGRF, Melbourne Australia
  Amplification Protocol: (Human 8K only)
  0.2mM dNTP, 25 pmols each primer, 2.5 units HotStar Qiagen TAQ, 1X HotStar buffer, and 1ul straight clone culture in 100ul reactions.
  95C for 15min; 7 cycles of 94C for 30s; 72C for 1min (-1C every cycle); 72C for 2min; 23 cycles of 94C for 30s; 65C for 1min; 72C for 2min; final elongation 72C for 5min; 4C 2min.
  Millipore PCR plate purification, gel analysis, aliquot into 384 well plates by robot (A1,A2,B1,B2).
  Resuspended in water for 4 days in fridge, then added an equal amount of DMSO.
  Printing concentration was ~250 ng/ul in 50% DMSO, 6ul total.
  Please see GAL file for sequence information and accession numbers for individual clones.
  Post Processing: UV crosslinked 70mj; baked 2hrs at 80C; stored dessicated.
  Feature Information:
  ~150um diameter
  Covalent and ionic attachment.
  All other information, please see GAL file.
  Control Elements on the array (see location on GAL file):
  Amersham "Lucinda Universal Scorecard version 2" spots. Spike mix available from the AGRF