title: WUSTL Streptococcus pyogenes 3K
status: Public on Apr 01 2007
contact.city: St. Louis
contact.first: Michael
contact.middle: G
contact.last: Caparon
contact.state: MO
contact.address: 660 S. Euclid Ave.
contact.department: Dept. of Molecular Microbiology
contact.country: USA
contact.web_link: http://www.caparonlab.wustl.edu
contact.institute: Washington University School of Medicine
organism: Streptococcus pyogenes
description: An antisense oligonucleotide array based on the published genome of S. pyogenes SF370 (AE004092.1  GI:14286347) was made at the Microarray Core Facility in the Genome-Sequencing Center at Washington University (GSC). A single 60–80 mer oligonucleotide was designed to hybridize to the putative transcripts from each annotated open reading frame using 'TOP' software that was created at the GSC. To reduce complexity, probes for rRNA, tRNA and prophage-associated genes were not included, which resulted in an array consisting of probes for 1517 genes out of a total of 1752 predicted open reading frames in the SF370 genome.
manufacture_protocol: Oligos were synthesized using standard methods by Illumina (San Diego, CA). These oligonucleotides were dissolved at a concentration of 50 μM in 3X SSC with 0.75M betaine and were printed in duplicate on MWG Epoxy slides by a locally constructed linear servo arrayer (after the DeRisi model, see the DeRisi lab web page at UCSF).