title: Legionella pneumophila 0.1K predicted small RNA
status: Public on Jan 01 2010
contact.city: St-Anne-de-Bellevue
contact.first: Sebastien
contact.last: Faucher
contact.email: sebastien.faucher@gmail.com
contact.state: QC
contact.address: 21,111 Lakeshore
contact.department: Natural Resource Sciences
contact.country: Canada
contact.institute: McGill University
contact.phone: 514-398-7886
organism: Legionella pneumophila subsp. pneumophila str. Philadelphia 1
manufacture_protocol: Probes were designed for the detection of 101 predicted small RNAs of Legionella pneumophila by using OligiWiz version 2.1.3. The prokaryotic setting was used to design 50-mers probes located, when possible, at the 3’ end of the predicted small RNA. Probes for 10 negative controls, representing 10 genes of the L. pneumophila Paris plasmid, were also designed. Probes (Illumina) were dissolved in 50% DMSO to a final concentration of 30 uM and printed in triplicate on UltraGAPS coated glass slides (Corning) using a PerkinElmer SpotArray microarray printer.