title: NIAID - Mmbf
status: Public on Mar 03 2004
contact.city: Bethesda
contact.first: Michael
contact.last: Wilson
contact.email: mwilson@ambion.com
contact.state: MD
contact.address: 50 South Drive, Room 5509
contact.department: Division of Intramural Research
contact.country: USA
contact.web_link: http://madb.niaid.nih.gov/
contact.institute: National Institute of Allergy and Infectious Diseases
contact.phone: 301-496-4287
organism: Mus musculus
description: Printing by NIAID / Microarray Research Facility
  Surface type: non-adsorptive, epoxy-coated glass
  Attachment: covalent
  Array description: The custom microarrays are manufactured by the Microarray Research Facility of the National Institute of Allergy and Infectious Diseases (NIH/NIAID). Microarrays are printed with an Omnigrid robotic arrayer by Genomic Solutions (http://www.genomicsolutions.com ) that is fitted with a printhead and 48 SMP3 print tips from Telechem (http://www.arrayit.com ). The mouse microarray (Mmbf) probe set is based on 70mer oligonucleotides from Qiagen's Mouse Genome Oligo Set V2.0 (http://oligos.qiagen.com/arrays/omad.php ).  A comprehensive gene list is also available at http://madb.niaid.nih.gov/
  Oligonucleotide probes are distributed into 384-well polypropylene plates (Genetix, 384 micro array) and dissolved to a normalized 25 micromolar concentration in print buffer (150mM sodium phosphate, pH 8.5, 4% sodium sulfate, 0.0001% N-lauroylsarcosine).  The Mmbf microarray spot densities are 210 microns (center-to-center).  The microarrays are printed on MWG Epoxy Slides (MWG Biotech Inc., # 1150-000000) and treated by a method that is an adaptation of the manufacturer's instructions to allow higher throughput processing.