title: AppChip1 (NRC-IBS Actinobacillus pleuropneumoniae L20)
status: Public on Apr 13 2006
contact.city: Ottawa
contact.first: John
contact.middle: H
contact.last: Nash
contact.email: John.Nash@gmail.com
contact.state: Ontario
contact.address: 100 Sussex Drive
contact.department: Institute for Biological Sciences
contact.country: Canada
contact.institute: National Research Council
organism: Actinobacillus pleuropneumoniae
description: PCR primers were designed for 2025 ORFs from a draft genomic sequence of Actinobacillus pleuropneumoniae L20 which comprised 2091 ORFs on 66 contigs. For AppChip1, all genes are spotted in duplicate on the microarray and sheared total genomic DNA from A. pleuropneumoniae L20 and porcine DNA were spotted in the top row of each subarray as controls.
manufacture_protocol: Primers were synthesized at the Biotechnology Research Institute (BRI-NRC) in Montreal. ORF-specific amplicons were produced from 30 ng of genomic DNA template by standard PCR amplification methods. Amplicon quality was evaluated by agarose gel electrophoresis. Amplicons were purified, lyophilized, and sent to BRI-NRC for printing onto microarray slides.