title: NimbleGen Gibbs Drosophila mojavensis 135K 12-plex Array
status: Public on Jan 15 2013
contact.city: Las Vegas
contact.first: Allen
contact.middle: G.
contact.last: Gibbs
contact.email: allen.gibbs@unlv.edu
contact.state: Nevada
contact.address: 4505 S Maryland Parkway
contact.department: School of Life Sciences
contact.country: USA
contact.institute: University of Nevada
contact.phone: 702-895-3203
organism: Drosophila mojavensis
description: The probe selection was based on the sequences from ftp://flybase.net/genomes/Drosophila_mojavensis/current/fasta/dmoj-all-transcript-r1.3.fasta.gz downloaded on 4/14/2009. Nine probes per transcript were selected. The report.txt file will give you the details of how many probes were chosen for each transcript. There is a total of 130705 probes (12-plex design hold 135K probes. The empty spaces were filled with random (negative) probes.) OID21283_rank9.select is a tab-delimited file containing each probes' sequence as well as other information about the probe (score, tm, etc.) Report.txt tells you the number of probes we were able to select for each id. No_probes.txt contains a list of what we weren't able to select a probe for. Exemplars.txt will let you know if one probe set represents multiple ids (for ex. When doing the selection, FBtr0160978 and FBtr0160979 shared the same probes, so FBtr0160979 was filtered out of the probe set). Finally, annotation.txt is the annotation file we were able to pull together based on the file we downloaded.
manufacture_protocol: Details on the manufacture protocol can be found on http://www.nimblegen.com/technology/index.html