title: Beaudet mouse PWS/AS strand-specific microarray
status: Public on May 03 2012
contact.city: Houston
contact.first: Linyan
contact.last: Meng
contact.email: lmeng@bcm.edu
contact.state: TX
contact.address: One Baylor Plaza
contact.department: Molecular and Human Genetics
contact.country: USA
contact.institute: Baylor College of Medicine
organism: Mus musculus
description: Agilent eArray online tool was used to design the array (SurePrint G3 Custom CGH Microarray 8x60K, 60-mer). High quality Agilent probes from chr7:65,910,585-67,600,789 (Snrpn-Ube3a, mm9), chr6:125,111,870-125,115,601 (Gapdh), chrX:103,382,463-103,399,038 (Pgk1), chr16:32,609,107-32,632,876 (Tfrc), and chr5:143,664,795-143,668,403 (Actb) were selected by eArray to generate probe group 1 (representing high quality probes). Genomic tilling probes for the Snrpn-Ube3a region were also generated to form probe group 2 ( named with "CUST", representing low quality probes). The default Agilent probes are from the plus strand. These two probe groups were then used to generate the reverse-complementary probes named with "RC" followed by their original probe name. These are the probes from the minus strand.
manufacture_protocol: http://www.genomics.agilent.com/collectionsubpage.aspx?pagetype=product&subpagetype=productdetail&pageid=1459