title: NIAID - Mta[a,b,c]
status: Public on Aug 10 2004
contact.city: Bethesda
contact.first: Michael
contact.last: Wilson
contact.email: mwilson@ambion.com
contact.state: MD
contact.address: 50 South Drive, Room 5509
contact.department: Division of Intramural Research
contact.country: USA
contact.web_link: http://madb.niaid.nih.gov/
contact.institute: National Institute of Allergy and Infectious Diseases
contact.phone: 301-496-4287
organism: Mycobacterium tuberculosis
description: Printing by NIAID / Microarray Research Facility
  Surface type: non-adsorptive, polylysine-coated glass
  Attachment: covalent, (UV-treated polylysine)
  Array description: The custom microarrays are manufactured by the Microarray Research Facility of the National Institute of Allergy and Infectious Diseases (NIH/NIAID). Microarrays are printed with an Omnigrid robotic arrayer by GeneMachines (http://www.genemachines.com) that is fitted with a printhead and 16 SMP5 print tips from Telechem (http://www.arrayit.com). The M. tuberculosis microarray (Mtaa) probe set is based on 70mer oligonucleotides from Qiagen's Tuberculosis Genome Oligo Set V1 (http://oligos.qiagen.com/arrays/omad.php). Oligonucleotide probes are distributed into 384-well polypropylene plates (Genetix, 384 micro array) and dissolved in 4XSSC print buffer at a normalized 25 micromolar concentration. The Mtaa microarray spot densities are 250 microns(center-to-center). The microarrays are printed on poly-L-lysine coated slides and post-processed according to the procedures on the Stanford website: http://cmgm.stanford.edu/pbrown/protocols/index.html