title: MBPL human 30k P9
status: Public on Mar 29 2013
contact.city: Hsinchu
contact.first: ChengWei
contact.last: Chang
contact.email: d924546@oz.nthu.edu.tw
contact.address: 101, Section 2 Kuang Fu Road
contact.department: bmes
contact.country: Taiwan
contact.institute: nthu
organism: Homo sapiens
manufacture_protocol: Arrayer obtained from Dr. Patrick O. Brown MGuide II and ArrayMarker program version 2.3.2 (Dr. Joseph DeRisi, university of California, San Francisco) with 48 spotting pins (TeleChem).We control printing condition in temperature 25~26°C and Humidity about 49~51%, and printing on January 2009. cDNA clones were purchased from Incyte Genomics and 7334 sequence-verified clones were amplified by PCR.We purified these 7334 clones by isopropanol precipitation and used 3× saline-sodium citrate (SSC) as spotting solution. cDNA spotted on GAPS II coated slides (Corning Incorporated) coating of pure gamma amino propylsilane.