title: Cambridge University Toxoplasma gondii 12k cDNA array
status: Public on May 22 2008
contact.city: Stanford
contact.first: Jon
contact.last: Boyle
contact.email: boylej@stanford.edu
contact.state: CA
contact.address: 299 Campus Drive
contact.department: Microbiology and Immunology
contact.country: USA
contact.institute: Stanford University School of Medicine
organism: Toxoplasma gondii
description: GAPS II (Corning)
manufacture_protocol: Approximately 12,000 cDNA clones were PCR amplified, resuspended in 3X SSC, 1.5M betaine and printed onto GAPS II slides (Corning, Netherlands), using a Lucidea Pro array printer (GE Healthcare Bio-Sciences, Amersham, UK) with a 48-pin tool. The slides were baked for 18 hours at 80°C and stored dessicated at room temperature. For 7,488 of the cDNAs, end-sequence data was obtained previously and is available in Genbank