title: University of Minnesota Custom miRNA microarray
status: Public on Nov 01 2012
contact.city: Nanjing
contact.first: Yan
contact.last: Zeng
contact.email: zengyan@njau.edu.cn
contact.state: Jiangsu
contact.address: XuanWu District, No.1 WeiGang
contact.country: China
contact.institute: Nanjing Agricultural University
organism: Caenorhabditis elegans
  Drosophila melanogaster
  Danio rerio
  Homo sapiens
  Mus musculus
  Rattus norvegicus
manufacture_protocol: A miRNA probe set was purchased from Invitrogen, which was designed based on the Sanger miRBase Sequence Database, Release 9.0.  The set contains approximately 1,140 oligonucleotides of 34 to 44 nt long as probes.  They are complementary to C. elegans, Drosophila, zebrafish, mouse, rat, and human miRNAs, and also include a number of internal and negative control probes.  The oligonucleotides were dissolved in 3 x SSC and quadruply printed on Corning® GAPSTM II coated slides  using a BioRobotics Microgrid II spotter