title: RayBiotech Human L493 Array, Membrane (AAH-BLM-2)
status: Public on Dec 09 2015
contact.city: Boston
contact.first: Ben
contact.middle: S
contact.last: Wittner
contact.email: wittner.ben@mgh.harvard.edu
contact.state: MA
contact.address: 185 Cambridge Street
contact.department: Cancer Center
contact.country: USA
contact.institute: Massachusetts General Hospital
contact.phone: +1-617-643-3166
organism: Homo sapiens
description: Antibody Array (Membrane)
manufacture_protocol: Recent technological advances by RayBiotech have enabled the largest commercially available antibody array to date. With the RayBio L-Series Human Antibody Array 507 or 493, researchers can now obtain a broad, panoramic view of cytokine expression. The expression levels of 507 or 493 human proteins can be simultaneously detected, including cytokines, chemokines, adipokine, growth factors, angiogenic factors, proteases, soluble receptors, soluble adhesion molecules and other proteins in cell culture supernatants. The first step in using the Human L-507 or L-493 is to biotinylate the primary amine of the proteins in the sample. The membrane arrays are then blocked, similar to a Western blot, and the biotin-labeled sample is added onto the membrane array which is pre-printed with capture antibodies and incubated to allow for interaction of target proteins. After incubation with HRPConjugated Streptavidin, the signals can be visualized by chemiluminescence. More information can be found at http://www.raybiotech.com/files/manual/Antibody-Array/AAH-BLM-1.pdf