title: UNMC-MWG Human 10K v1.0
status: Public on Jul 20 2012
contact.city: Omaha
contact.first: Mark
contact.middle: A.
contact.last: Carlson
contact.email: macarlso@unmc.edu
contact.state: NE
contact.address: Surgery 112, VA Medical Center, 4101 Woolworth Ave
contact.department: Surgery
contact.country: USA
contact.web_link: http://www.nebraskasurgicalresearch.com
contact.institute: Univ Nebraska Med Ctr
contact.phone: 402-995-5371
organism: Homo sapiens
manufacture_protocol: The gene clones on the chip consisted of unique 50-mer oligonucleotide sense strand sequences synthesized by MWG Biotech (Ebersberg, Germany). The oligo set from MWG consists of approximately one third of the genes expressed in the human genome (9,850 genes) with 30 replica spots and 104 Arabidopsis negative controls. Each gene is annotated with a specific NCBI accession number (release 126). The oligos were suspended in 3X SSC in 384 V bottom plates and were spotted to the polylysine coated glass slides by a set of 16 robotically controlled printing tips using a Omnigrid microarray printer in the UNMC Microarray Core Facility.