title: Goettingen_Univ_S. cerevisiae_Array-Ready Oligo Set V1.1
status: Public on Nov 30 2012
contact.city: Goettingen
contact.first: Gabriela
contact.last: Salinas
contact.email: Gabriela.Salinas-Riester@medizin.uni-goettingen.de
contact.state: Lower-Saxony
contact.address: Kreuzbergring 57
contact.department: Department of Pathology
contact.country: Germany
contact.institute: Universitaetsmedizin Goettingen
organism: Saccharomyces cerevisiae
manufacture_protocol: The array was constructed by spotting a 15µM solution of 5’ amino-C6-modified oligonucleotides in 50mM sodium phosphate pH 8.5 with a length of 70 bases on CodeLink microarray slides (SurModics, Eden Prairie, MN, USA) using a MicroGrid II microarray spotter (Zinsser Analytic, Frankfurt, Germany). Each spot contains around 1.5fmol of oligonucleotide. Oligonucleotides were covalently coupled to the slide surface and blocked using ethanolamine according to the instructions of the supplier. The array consists of 6388 probes, as provided by the Array-Ready Oligo Set V1.1 for S. cerevisiae (Eurofins). For additional information see manufacturer's website.