title: VUMC MACF Human 30K oligo (batch68.gal)
status: Public on Feb 01 2011
contact.city: Cambridge
contact.first: Nicholas
contact.middle: B
contact.last: Shannon
contact.address: Robinson Way
contact.department: Oncology
contact.country: United Kingdom
contact.institute: Cambridge Research Institute
organism: Homo sapiens
manufacture_protocol: The Human Release 2.0 oligonucleotide library, containing 60-mer oligonucleotides representing 28830 unique genes as designed by Compugen (San Jose, CA, USA), was obtained from Sigma-Genosys (Zwijndrecht, The Netherlands). The oligonucleotides were dissolved at 10 μM concentration in 50 mM sodium phosphate buffer pH 8.5 and single spotted onto CodeLink™ slides (Amersham Biosciences), using an OmniGrid® 100 microarrayer (Genomic Solutions, Ann Arbor, MI, USA) equipped with SMP3 pins (TeleChem International, Sunnyvale, CA, USA). After printing slides were processed and blocked prior to use according to the manufacturer’s protocol.