title: Zebra finch brain cDNA microarray (v1)
status: Public on Nov 09 2006
contact.city: New York
contact.first: XiaoChing
contact.last: Li
contact.email: lixc@rockefeller.edu
contact.state: NY
contact.address: 1230 York Avenue
contact.country: USA
contact.institute: Rockefeller University
contact.phone: (212) 327-8381
organism: Taeniopygia guttata
manufacture_protocol: Based on the redundancy analysis, highly redundant clones were removed, and in the re-assembled printing set, each gene transcript was represented one or two times.  The cDNA inserts were PCR amplified.  The PCR products were purified, checked by gel electrophoresis and quantified by UV absorption.  The DNA samples were dissolved in 35% DMSO and printed in duplicate onto glass slides (UltraGAPS, Corning) using the MicroGrid (BioRobotics).  Slides were then cross-linked by UV radiation at 360J.