title: Wurdinger/Krichevsky miRNA array IV
status: Public on Oct 08 2008
contact.city: Charlestown
contact.first: Thomas
contact.last: Wurdinger
contact.email: twurdinger@mgh.harvard.edu
contact.state: MA
contact.address: 149 13th Street
contact.country: USA
contact.institute: MGH
organism: Homo sapiens
  Mus musculus
  Rattus norvegicus
manufacture_protocol: An expanded miRNA array was developed as described (Krichevsky et al., 2003) with modified probe design to balance Tm of hybridization to all miRNAs. A total of 407 tri-mer DNA oligonucleotides (anti-sense to microRNAs [Sanger miRBase version 8.0]) were spotted onto GeneScreen Plus (NEN) membranes at a final concentration of 5 µM with a 1536 pin plate replicator (V&P Scientific).