title: DKFZ Division of Molecular Genome Analysis Human Operon 4.0 oligo Array 35k
status: Public on Sep 17 2008
contact.city: Heidelberg
contact.first: Alberto
contact.last: Calabro
contact.address: INF 580
contact.department: B050
contact.country: Germany
contact.institute: DKFZ
organism: Homo sapiens
manufacture_protocol: The microarrays contains 35,035 oligonucleotide probes, representing approximately 25,100 unique genes and 39,600 transcripts excluding control oligos spotted on epoxysilane glass slides (Quantifoil, Jena, Germany) using the VersArray ChipWriter Pro System (Biorad, Munich, Germany) and SMP3 pins (Telechem, Sunnyvale, CA, USA). Spotting was performed in the division of Molecular Genome Analysis with a spacing of 28x29 Spots in 4x12 Subarrays (top-down to left-right). After spotting, microarrays were rehydrated, and the DNA was denatured with boiling water prior to washing with 0.2% SDS, water, ethanol, and isopropanol. The arrays were dried with air pressure.