title: MBPL human 30k P2
status: Public on Jun 27 2006
contact.city: Hsinchu
contact.first: ChengWei
contact.last: Chang
contact.email: d924546@oz.nthu.edu.tw
contact.address: 101, Section 2 Kuang Fu Road
contact.department: bmes
contact.country: Taiwan
contact.institute: nthu
organism: Homo sapiens
description: There are 32448 spots within a array.7334 human clone were printing 4 duplicate in a array. Additionally, we printing 10 doping control genes (SpotReport™-10 Array Validation System , Stratagene)  and one house-keeping gene , 5 negative control gene as quality control indicator. total 16 quality control indicator were printing 2 duplicate in each block.
manufacture_protocol: Arrayer obtained from Dr. Patrick O. Brown MGuide II and ArrayMarker program
  
  version 2.3.2 (Dr. Joseph DeRisi, university of California, San Francisco) with 48
  
  spotting pins (TeleChem).We control printing condition in temperature 25~27°C and Humidity about 41~43%, and printing on April 2003.
  
  cDNA clones were purchased from Incyte Genomics and 7334 sequence-verified
  
  clones were amplified by PCR.We purified these 7334 clones by isopropanol
  
  precipitation and used 3×saline-sodium citrate (SSC) as spotting solution. cDNA spotted on GAPS Ⅱ coated slides (Corning Incorporated)coating of pure
  
  gamma amino propylsilane.