title: IOLR Lithognathus mormyrus 4608 v2.0
status: Public on Dec 01 2009
contact.city: Haifa
contact.first: Moshe
contact.last: Tom
contact.email: tom@ocean.org.il
contact.address: P.O.B. 8030
contact.department: Marine Biology and Biotechnology
contact.country: Israel
contact.web_link: http://www.ocean.org.il/
contact.institute: Israel Oceanographic and Limnological Research
contact.phone: 972-4-8565-257
organism: Lithognathus mormyrus
description: Two identical arrays were printed on one 2.5 x 7.5 cm glass (GAPS II slides, Corning) on an area less than 6 x 2 cm. The grid system of each arrray includes 4 x 6 sub-arrays, each containing 9 rows and 15 columns, a total of 1620 unique spots in duplicates, totaling 3240 spots. Not all the spots contained usable DNA.
manufacture_protocol: The protocol for preparation of the array is detailed in: Auslander, M., Yudkovski, Y., Chalifa-Caspi, V., Herut, B., Ophir, R., Reinhardt, R., Neumann, P.M., Tom, M., 2008. Pollution-affected fish hepatic transcriptome and its expression patterns upon exposure to cadmium. Mar. Biotechnol. 10, 250-261. However, the number of printed clones was reduced to 1152, maintaining the original designations of the platform. Two arrays were printed on one slide, each containing duplicates of the printed clones.