title: KTH_E.Coli_6k_Oligo_Microarray
status: Public on Jan 23 2009
contact.city: Tallinn
contact.first: Ranno
contact.last: Nahku
contact.email: ranno@tftak.org
contact.address: Akadeemia tee 15b
contact.country: Estonia
contact.institute: TFTAK
organism: Escherichia coli
manufacture_protocol: The E. coli Genome Oligo Set Version 1.0 consists of 5978 70mer probes representing tree strains of Escherichia coli. The number of open reading frames (ORFs) represented is as follows: 4289 ORFs in K12; 5059 ORFs in O157:H7(EDL933); 4836 ORFs in O157:H7Sakai; All 5978 probes are designed within ORFs from both cloned genes and predicted ORFs. For our probe design, we use state-of-the-art methods and proprietary software.
  All probes are Tm normalized to 75ºC (±5ºC) and have a 5' amino linker.
  http://www.operon.com/arrays/oligosets_ecoli.php
  
  Array manufacturing
  The 5978 70mer probes were dissolved in Universal Spotting Solution (Corning) to a concentration of 18 µM. The microarrays were printed with a QArray (Genetix) instrument with 24 PT2500 pins (Point Technologies) on Ultra GAPS slides (Corning). The 5978 70mer probes were spotted in a 17x17 pattern within each block and with a feature center-to-center distance of 250 µm in two identical fields. The quality of the spotted slides was assessed by staining with Syto61 (Molecular Probes) and with hybridization with random nonamers. The slides were UV cross-linked at 150 mJ/cm2.