title: Arabidopsis thaliana small RNAs sequences identified using high-throughput 454 sequencing technology
status: Public on Feb 01 2007
contact.city: Saint Louis
contact.first: James
contact.middle: C
contact.last: Carrington
contact.email: jcarrington@danforthcenter.org
contact.state: MO
contact.address: 975 North Warson Road
contact.country: USA
contact.institute: Donald Danforth Plant Science Center
contact.phone: 314-587-1202
organism: Arabidopsis thaliana
description: The dataset contains unique, 20-25 nucleotide small RNA sequences identified using high-throughput 454 sequencing of samples prepared from Arabidopsis thaliana.  Each sequence has a minimum of one genomic locus.  Small RNAs were sequenced from inflorescence tissue (containing stage 1-12 flowers) of wildtype Col-0 plants, mutants with defects in each DCL gene (dcl1-7, dcl2-1, dcl3-1, dcl4-2), mutants with defects in each RDR gene for which a function has been established (rdr1-1, rdr2-1, rdr6-15), and whole seedling tissue (3 day post-germinations) of Col-0 and rdr6-15 plants.  Small RNA preparations from leaf samples of Col-O that were either uninoculated or inoculated by Pseudomonas syringae pv tomato (DC3000hrcC) for 1 hr and 3 hr were also sequenced.