title: Agilent-030208/UofA Cow 8x15K miRNA array v.3
status: Public on Jul 01 2012
contact.city: Edmonton
contact.first: Josue
contact.middle: Moura
contact.last: Romao
contact.state: Alberta
contact.address: 116 St. and 85 Ave.
contact.department: AFNS
contact.country: Canada
contact.institute: University of Alberta
organism: Bos taurus
description: Slide format (1'' x 3''), 8x15K format,
  miRbase v.15
manufacture_protocol: Microarrays are manufactured using a proprietary non-contact industrial inkjet printing process, in which oligo monomers are deposited uniformly onto specially-prepared glass slides. This in situ synthesis process prints 60-mer length oligonucleotide probes, base-by-base, from digital sequence files. The precise inkjet process enables the delivery of extremely small, accurate volumes (picoliters) of the chemicals to be spotted. Standard phosphoramidite chemistry used in the reactions allows for very high coupling efficiencies to be maintained at each step in the synthesis of the full-length oligonucleotide. Precise quantities are reproducibly deposited. This process is achieved without stopping, to make contact with the slide surface and without introducing surface-contact feature anomalies, resulting in consistent spot uniformity and traceability.