title: Medicago truncatula 1K array
status: Public on Oct 14 2005
contact.city: St Paul
contact.first: Kathryn
contact.middle: A
contact.last: VandenBosch
contact.email: vande102@umn.edu
contact.state: MN
contact.address: Room 250 BioSci 6022 1445 Gortner Ave
contact.department: Plant Biology
contact.country: USA
contact.institute: University of Minnesota
contact.phone: +1 612-624-2755
organism: Medicago truncatula
description: The cDNA set used for preparing this array contained a total of 1,152 clones (kiloclone set) selected by sequence analysis of M. truncatula ESTs.  Each clone is spotted in triplicate. The array contains clones from 15 M. truncatula cDNA libraries that had been 5’-end sequenced at the Institute for Genomic Research (TIGR). These libraries were generated from diverse developmental stages with emphasis on vegetative organs interacting with microbes and comparable uninfected stages: seedling roots, roots inoculated with Sinorhizobium meliloti, mature nodules and senescent nodules, mycorrhizal roots, phosphate-starved roots, Phytophthora medicaginis-infected roots, glucan-elicited roots, oligogalacturonide-elicited roots, Colletotrichum trifolii-infected leaves and cotyledons, and healthy leaves and cotyledons. The set also included organ-specific controls, positive controls (genes known to be expressed) for microbial responses, and genes with putative function as cell cycle/cell death regulators, regulators of cell organization and morphogenesis, transcription factors, metabolic enzymes as well as several genes with unknown function or no hits in GenBank.
manufacture_protocol: arrayer hardware -
  Gene Machine OmniGrid array spotter