title: Qiagen Human Serum & Plasma microRNA PCR array plate (MIHS-106Z)
status: Public on Aug 03 2022
contact.city: São Paulo
contact.first: Talita
contact.middle: Souza
contact.last: Siqueira
contact.email: talitablz@hotmail.com
contact.state: São Paulo
contact.address: Galvão Bueno, 868 - Liberdade
contact.department: Interdisciplinary Post-Graduate Program in Health Sciences
contact.country: Brazil
contact.institute: Cruzeiro do Sul University
contact.phone: 5513988383934
organism: Homo sapiens
description: PCR assay
  Extracellular Vesicle miRNA concentrations were measured using the Human Serum & Plasma microRNA PCR array plate (MIHS-106Z, Qiagen, Hilden, Düsseldorf, Germany).
manufacture_protocol: For the PCR array analysis, 12.5 µL SYBR Green PCR Master Mix, 2.5 µL Universal Prime (10×), 9 µL RNase water-free and 1 µL pre-amplified cDNA (total volume=25 µL) was added to each well of the miRNA PCR Array plate. The plate was centrifuged at 1,000×g for one minute at room temperature. Data were recorded with a QuantStudio 3 device and analyzed using the QuantStudio 3 Design and Analysis Software v1.5.1 (Thermo).