title: Arizona Microarray Qiagen-Operon Arabidopsis Genome Set 31K Version 3.0
status: Public on Jan 25 2008
contact.city: Goettingen
contact.first: Gabriela
contact.last: Salinas
contact.email: Gabriela.Salinas-Riester@medizin.uni-goettingen.de
contact.state: Lower-Saxony
contact.address: Kreuzbergring 57
contact.department: Department of Pathology
contact.country: Germany
contact.institute: Universitaetsmedizin Goettingen
organism: Arabidopsis thaliana
manufacture_protocol: - add 15 μL of 3XSSC to each well of the 300 pM oligonucleotide set (Qiagen-Operon Arabidopsis Genome Array Ready Oligo Set Version 3.0) to make a 20 μM oligonucleotide solution
  - transfer onto an orbital shaker for 1 h at RT at 100 rpm. The oligonucleotides are now ready for printing.
  - Arabidopsis oligonucleotide microarrays are printed on aminosilane-coated “Superamine” slides (Telechem)
  - Printing conditions:
  Single array element printed per oligonucleotide, 48 pins in the print head, 31% relative humidity, redipping after every 50 slides.  The print head Z- velocity is set to a maximum of 10 mm/sec.
  - Bake slides for 2h at 80oC. Store dry and in darkness at RT.