title: Exiqon miRCURY LNA™ microRNA Array [Cat# 208210-A; miRBase v.11.0]
status: Public on Apr 02 2019
contact.city: Stillwater
contact.first: CHAOQUN
contact.last: HUANG
contact.email: chaoqh@okstate.edu
contact.state: OK
contact.address: 264 McElroy Hall
contact.department: Physiological Sciences
contact.country: USA
contact.institute: Oklahoma State University
contact.phone: 405-744-3410
organism: Homo sapiens
  Mus musculus
  Rattus norvegicus
manufacture_protocol: miRCURY LNA™ microRNA Array ready-to-spot probe set 11.0 were purchased from EXIQON Cat#208210-A).Capture probes complementary to mature microRNAs for all microRNAs in human, mouse, rat and their related viruses registered in miRBase Release Version 11.0. The capture probes are Locked Nucleic Acid (LNA) enhanced oligonucleotides. All probes are Tm-normalized and amino modified.Dissolve the capture probes in 15 μL printing buffer resulting in a final concentration of 20 μM capture probe during spotting. The probes were then printed onto epoxy-coated slides (CEL Associates, Pearland, TX) with an OmniGrid 100 array (GeneMachine, San Carlos, CA) at 65% humidity and then incubated for 48 hours at the same humidity. Each slide contained three identical blocks in a landscape orientation. Within each block every probe was printed  in duplicates.