title: Immunity Evaluation Autoarray
status: Public on May 30 2018
contact.city: Chongqin
contact.first: Xiaodong
contact.last: Xie
contact.email: xiexiaodong10@yeah.net
contact.address: 30, gaotanyan Street, shapingba District
contact.country: China
contact.institute: Army medical university
organism: Mus musculus
description: mice immunity evaluation PCR assay panel
manufacture_protocol: High throughput RT-qPCR was carried out on ViiA7 platform (Life technology, USA) in a total reaction volume of 2.1μL (including 1.6μL BRYT Green mix, 0.15μL primer, and o.2μL reverse transcription product) according to the manufacturer’s protocol (Promega). The Echo 550 liquid handler (Labycte, USA) was used for liquid handling. The reactions were initiated in a 384-well optical plate at 95℃ for 5 min, followed by 40 cycles of 95℃ for 30s and 60℃ for 45s. Limma was used to identify genes that are differently expressed between experimental conditions