title: Agilent/CCFFT Lactococcus lactis IL1403 8x15k v1.0 (gene name version)
status: Public on Jan 11 2011
contact.city: Tallinn
contact.first: Ranno
contact.last: Nahku
contact.email: ranno@tftak.org
contact.address: Akadeemia tee 15b
contact.country: Estonia
contact.institute: TFTAK
organism: Lactococcus lactis subsp. lactis Il1403
description: Steps/settings in Earray (http://www.chem.agilent.com/en-US/products/instruments/dnamicroarrays/pages/gp50660.aspx):
  a. Design Wizards - Create a Microarray Design from Target Transcripts
  b. Selected Method - Tm matching methodology
  c. Selected Parameters:
  Probe Length - 60bp
  Probes per Target - 7
  Probe Orientation - Sense
  Design Options - Best Distribution Methodology
  NO - "Design with 3' Bias" and "Allow Probes to be Trimmed"
  Preferred Probe Tm - 85C
  Target File Details - Upload Gene Sequence File
  Note: "l.lactis.nuc" file was downloaded from the KEGG ftp site (ftp://ftp.genome.jp/pub/kegg/genes/organisms/lla/ )
  Transcriptome Details - Use Target File as Transcriptome
  d. 8x15K platform was used
  e."Fill Microarrays" option was checked
manufacture_protocol: Described in: http://www.chem.agilent.com/en-us/products/instruments/dnamicroarrays/pages/gp557.aspx