title: Custom PEPperCHIP Peptide Microarray
status: Public on Feb 28 2015
contact.city: Birmingham
contact.first: Jennie
contact.last: Hamilton
contact.state: AL
contact.address: 1825 University Blvd.
contact.department: Department of Medicine, Division of Clinical Immunology and Rheumatology
contact.country: USA
contact.institute: University of Alabama Birmingham
organism: Homo sapiens
description: Laser printer spotted peptides (peptide autoantigens).
manufacture_protocol: Amino acid microparticles are laser printed onto a glass slide with a custom 24-color laser printer.  One layer of an amino acid particle pattern is printed on a coated glass slide. A heating step melts the particles.  Embedded amino acids can couple to the chip surface.  In accordance with solid phase Fmoc chemistry, a cycle of synthesis is completed after removal of excessive material by washing and cleavage of the N-terminal Fmoc protection group. The cycle is repeated until the full peptide microarray is synthesized.