title: Osaka University E. coli O157 Sakai Oligo Array 5.5K ver2
status: Public on Feb 01 2008
contact.city: Suita
contact.first: Toru
contact.last: Tobe
contact.email: torutobe@bact.med.osaka-u.ac.jp
contact.state: Osaka
contact.address: 2-2 Yamadaoka
contact.department: Graduate School of Medicine
contact.country: Japan
contact.institute: Osaka University
contact.phone: +81-6-6879-3301
organism: Escherichia coli
manufacture_protocol: Oligonucleotide probes were prepared for all the protein-coding genes on the O157 Sakai genome (5,447 genes in total).  Principles for the probe design were 60-mer in length and one probe per gene/CDS.  For each gene, we designed 60-mer probe except one 44 or 45-mer probes for 7 genes.  In total, 5447 probes were synthesized (Sigma-Aldrich Japan, Tokyo, Japan), and each probe was spotted onto poly-L-Lysine coated glass slides (SD10011; Matsunami Glass Ind., Osaka, Japan) using SPBIO (Hitachi Software Engineering, Tokyo, Japan).