title: Agilent-085537 SurePrint G3 Custom GE 4x180K Microarray (Agilent, 9G4862A, 4×180K)
status: Public on Dec 21 2023
contact.city: Guangzhou
contact.first: Yingqin
contact.last: Li
contact.email: liyingq@sysucc.org.cn
contact.state: Guangdong
contact.address: Room 927, Building 2, No. 651 Road Dong Feng East
contact.country: China
contact.institute: Sun Yat-sen University Cancer Center
organism: Homo sapiens
manufacture_protocol: Sample labeling and array hybridization were performed according to the Agilent One-Color Microarray-Based Gene Expression Analysis protocol (Agilent Technology) with minor modifications. 1.65 μg of each labeled cRNA was fragmented by adding 11 μl 10 × Blocking Agent and 2.2 μl of 25 × Fragmentation Buffer, then heated the mixture at 60°C for 30 min, finally 55 μl 2 × GE Hybridization buffer was added to dilute the labeled cRNA. 100 μl of hybridization solution was dispensed into the gasket slide and assembled to the slide. The hybridized arrays were washed, fixed and scanned with using the Agilent DNA Microarray Scanner (part number G2505C). Agilent Feature Extraction software (version 11.0.1.1) was used to analyze acquired array images.