title: Mycobacterial 4100 oligo array (Operon Technologies)
status: Public on Jan 23 2014
contact.city: Faridabad
contact.first: Nisheeth
contact.last: Agarwal
contact.email: nisheeth@thsti.res.in
contact.state: Haryana
contact.address: 3rd Milestone, Faridabad-Gurugram Expressway
contact.department: Infection and Immunity
contact.country: India
contact.institute: Translational Health Science and Technology Institute
contact.phone: +911292876304
organism: Mycobacterium tuberculosis
manufacture_protocol: 1.Oligos are arrayed in 384-well flat-bottom plates. Each well contains 600 pmol of 70-mer oligo.
  2. Resuspend oligos in water to 20 µM and rearray 5 µL into 384-well, polystyrene V-bottom plates.
  3. Allow oligos to dry through passive water evaporation in a chemical hood.
  4. Before printing, add 5 µL of 3X SSC to each well. This can be done the night before a print run is started.
  5. Seal plates with Corning seals.
  6. Incubate at 37°C for 30 minutes to aid resuspension of DNA.
  7. Shake plates near maximum rotational speed on flat-bed shaker for 1 minute.
  8. Centrifuge plates at 500 rpm for 5 minutes.
  9. Remove seals and cover with plate lids. Place in appropriate location of plate cassette. This should be done with first plates just before print run is started to minimize evaporation time before printing.
  10. Make sure plates rest behind both holding clips in the cassettes. Push plates back into the cassettes as far as they will go, putting them in the proper position for the server arm.
  11. After the print run is completed, allow plates to dry through passive evaporation in a chemical hood.
  12. For each subsequent preparation of these plates for a print run, add water to the wells instead of SSC. The amount of water should be decreased by 0.25 µL per print run, as this is the amount drawn up by the pin capillary during each printing run.