title: Broad P. falciparum 328 v1
status: Public on Nov 15 2014
contact.city: Boston
contact.first: Daria
contact.last: Van Tyne
contact.address: 243 Charles Street
contact.country: USA
contact.institute: Harvard Medical School
organism: Plasmodium falciparum
manufacture_protocol: From Methods section of associated publication: nCounter® probe specificity is generated by designing two 50bp probes that sit next to one another, yielding 100bp total probe specificity for each gene of interest. Probe set design was carried out to maximize specificity and minimize cross-hybridization. Specifically, long direct and inverted repeats and long polynucleotide repeats were excluded, target regions were screened for cross-hybridization against the Human RefSeq mRNA database (http://www.ncbi.nlm.nih.gov/refseq/), and probes with stretches of more than 15 contiguous bases complementary to any non-target mRNA were excluded. Probes were also screened for inter- and intra-probe interactions, and were selected to have calculated melting temperatures (Tm) between 78 and 83°C, with an ideal target of 80.5°C. Finally, mismatch probes were also included in the nCounter® custom probe set, as part of the negative controls.
  Custom nCounter probes