title: NanoString JohnsonLab-Calbicans-72probe-v1.0
status: Public on Aug 28 2023
contact.city: San Francisco
contact.first: Matthew
contact.last: Lohse
contact.state: CA
contact.address: 44 Vicksburg Street
contact.country: USA
contact.institute: BioSynesis, Inc.
organism: Candida albicans
manufacture_protocol: Manufacturer (NanoString Technologies) was provided with a list of 68 genes and 4 UTRs from Candida albicans to design a custom oligonucleotide probe set targeting genes of interest (while avoiding introns). Manufacturer selected 100bp sequences with optimal GC content and predicted functional Tm’s (ideally 77-83 degrees Celsius).