title: AbC ELTE_human_960_v1
status: Public on Dec 01 2008
contact.city: Budapest
contact.first: József
contact.last: Prechl
contact.email: jprechl@iif.hu
contact.address: Pázmány Péter s. 1/C
contact.department: Immunology Research Group
contact.country: Hungary
contact.web_link: http://www.abc-arrays.com
contact.institute: ELTE-MTA
contact.phone: +3613812175
organism: Homo sapiens
description: Spotted antigens (protein, peptide, DNA … )
manufacture_protocol: Antigens were spotted in triplicates of three dilutions (1x, 5x, 125x) onto home made nitrocellulose chip. Dilutions were carry out in 0.05% Tween 20 containing PBS. BioOdyssey Calligrapher (Bio-Rad) was used for the printing with the following program:
  sourceselect Run = No
  substrate Type = Slides
  Number of Slides in Y= 4
  Number of Slides in X= 4
  Slide Size = standard
  Substrate Size in Millimeters in X= 75.500
  Substrate Size in Millimeters in Y= 25.350
  [Grid Layout]
  Source plate Type= 384
  Number of Source plate Rows= 8
  Number of Source plate columns= 12
  Number of Dips per Source plate= 96
  Pin Type= solid
  Pin Distance = 4.5mm
  Number of pins in Y= 2
  Number of pins in X= 2
  Number of samples = 256
  Number of Spots to be printed = 1536
  Replicate Spots per Grid in X= 1
  Replicate Spots per Grid in Y= 3
  Use Advanced parameters = Yes
  [Grid Advanced]
  Top Left Grid Margin in x= 5.000 (mm)
  Top Left Grid Margin in Y= 5.000 (mm)
  Bottom Right Grid Margin in x= 8.150 (mm)
  Bottom Right Grid Margin in Y= 5.000 (mm)
  Distance between Spots in x= 550.000 (micron)
  Distance between Spots in Y= 550.000 (micron)
  No. of Spots per Grid in X= 8
  No. of Spots per Grid in Y= 6
  No. of Total Spots per Grid= 48
  No. of Reprints of the Same Spot= 1
  Grid printing Direction= Left-right, Top-down
  No. of Different supergrids in x= 2
  No. of Different supergrids in Y= 2
  Total Number of supergrids= 4
  Distance between supergrids in x= 4.500 (mm)
  Distance between supergrids in Y= 4,500 (mm)
  Supergrid printing Direcrion= Left-right, Top-down
  No. of Duplicate supergrid clusters ln x= 2
  No. of Duplicate supergrid clusters in Y= 1
  Distance between Duplicate supergrid clusters in x= 17.675 (mm)
  - Distance between Duplicate supergrid clusters in Y= 4.500 (mm)
  No. of Full plates= 0
  No. of Dips in the Last plate= 64
  Total No. of Spots in a Grid= 48
  Sample picking Direction= Top-down, Left-right
  SubDips Exist= 0
  SubDip Rows= 1
  SubDip Columns= 1
  Total SUbDips= 1
  [Blotting]
  Use blots= No
  Blot slides Blot Distance= 450 (micron)
  All Blotting slides are sufficient for= 11.666667 (plates)
  [washing]
  wash selection= Different samples
  Pre-wash Step1= 2
  Pre-wash Step1 Time= 2.500 (s)
  Pre-wash step2= 1
  Pre-wash Step2 Time= 2.000 (s)
  Pre-wash Step3= 0
  Pre-wash Step3 Time= 0.000 (s)
  Main-wash cycles= 5
  Main-wash Step1= 3
  Main-wash Step1 Time= 3.000 (s)
  Main-wash Step2= 1
  Main-wash Step2 Time= 0.500 (s)
  Main-wash step3= 0
  Main-wash Step3 Time= 0.000 (s)
  Post-wash Step1= 3
  Post-wash Step1 Time= 3.000 (s)
  Post-wash Step2= 2
  post-wash Step2 Time= 5.000 (s)
  Post-wash step3= 1
  post-wash step3 Time= 0.500 (s)
  [options]
  Z-Down offset in sourceplate = 0.000 (mm)
  Source plate Approach speed= 20.000 (mm/s)
  Source plate Withdraw speed= 15.000 (mm/s)
  Source plate Dwell Time = 1000.000 (ms)
  oscillation in water-bath1 = Yes
  oscillation in Water-bath2 = Yes
  Water-bath1 Oscillation Distance in x= 4.000 (mm)
  water-bath2 Oscillation Distance in x= 1.500 (mm)
  water-bath1 Number of oscillations in Bath= 3
  water-bath2 Number of oscillations in Bath= 3
  Z-Down offset on slides = 0.000 (mm)
  Slide/Membrane/Blot Approach speed= 10.000 (mm/s)
  Dwell Time for printing= 25.000 (ms)
  Number of substrates between Redips = 0
  Number of Redips per sample = 1