title: Chipscreen/Operon Human Genome Array-Ready Oligo Set (Version 4.0)
status: Public on Mar 28 2017
contact.city: Hefei
contact.first: Rui
contact.last: Li
contact.email: lirui@ustc.edu.cn
contact.state: Anhui
contact.address: Huangshan Road 443, USTC West Campus
contact.department: School of Life Sciences
contact.country: China
contact.institute: University of Science and Technology of China
organism: Homo sapiens
manufacture_protocol: Human whole genomic gene probe set (Operon AROS Version 4.0) were purchased from Operon biotechnologies, Inc., which composed of 91 plates in 384-well format and 34944 distinct probes (detailed as in the microarray gene list).
  The PowerMatrix Slides were purchased from Fullmoon Biosciences, which designed to immobilize oligonucleotides for microarray applications. The slides were coated with proprietary polymer materials, which create a three dimensional matrix-like binding surface.
  The probes were dissolved in 2 × printing buffer (from Fullmoon Biosciences) at a final concentration of 1 µg/µL and then ready for microarray printing.
  The array format on the slide was composed of 48 small blocks (4 columns × 12 rows), each small block was composed of 729 probe spots (27 columns × 27 rows), so there are totally 34992 probe spots (48 blocks × 729 spots/block) on one slide. The diameter of each spot was about 125µM (plus 10% buffered area).
  After printing, the microarray slides were incubated in a chamber with 65 – 75% relative humidity for 10-12 hours. Then the slides were mobilized by UV cross link at 400 mJ. Finally allow the slides to dry at room temperature for 30 minutes and then ready for use.