title: PC Human Operon 21k v2
status: Public on Jul 28 2009
contact.city: Brisbane
contact.first: Glen
contact.middle: M
contact.last: Boyle
contact.email: Glen.Boyle@qimr.edu.au
contact.state: Queensland
contact.address: 300 Herston Road Herston
contact.department: Department of Cell and Molecular Biology, Cancer Program
contact.country: Australia
contact.institute: QIMR Berghofer Medical Research Insitute
contact.phone: +61 7 3362 0319
organism: Homo sapiens
manufacture_protocol: This array consists of 21,329 70mer probes representing 21,329 genes ( Operon Version 2.1 of the Human Genome Oligo Set). Oligonucleotides are consolidated into 384-well plates, lyophilized by speed-Vac, and resuspended in 3x SSC to a printing concentration of 30uM. Oligos are printed on aminosilane slides (Erie, Hudson, NH, USA) with a Biorobotics Microgrid II microarray printer (Genomic Solutions). Microspot 10K quill pins (Biorobotics) in a 48-pin tool were used to deposit <0.5 nL per spot onto the slide. The resulting microarrays have a 4 x12 subgrid layout with 392 spots per subgrid, each spot having approximate diameter and pitch of 90 µm and 190µm, respectively. A 280-bp GFP (green fluorescent protein) oligo was printed in subgrid corners to assist in placing on the grid. The slides were crosslinked in a UV Stratalinker 2400 (Stratagene) at 300mJ. Array quality was assessed by visual inspection and hybridization of representative slides from a print run by dye-labelled random 9-mer oligonucleotides. The quality control images were aquired via the ScanArray Express from Perkin Elmer at a 10 micron resolution and quantified with the Imagene 6.0 software suite.