title: CapitalBio CAU Rose 10K array
status: Public on Jan 09 2013
contact.city: Beijing
contact.first: Junping
contact.last: Gao
contact.email: gaojp@cau.edu.cn
contact.address: Department of Ornamental Horticulture, China Agricultural University
contact.department: Ornamental Horticulture
contact.country: China
contact.institute: China Agricultural University
contact.phone: 0086-10-62733848
organism: Rosa hybrid cultivar
manufacture_protocol: Five microliters of the purified PCR product from each clone was mixed with 5 μl of saline-sodium citrate (SSC) and transferred into 384-well plates (Greiner Bio-One, Frickenhausen, Germany). The concentration of probe DNA was adjusted to between 0.10 and 0.20 mg/mL. The prepared probe cDNAs were printed onto UltraGAPS coated slides (Corning, Corning, NY) using a MicroGrid II 600 spotter (BioRobotics, Cambridge, UK) with 16 MicroSpot 2500 printing pins. The spotting space was 250 μm and each DNA element was spotted in two replicates. SSC was used as negative control of array. Temperature and humidity inside the arrayer were maintained at 25 °C and 45% relative humidity, respectively. cDNAs were immobilized to the modified glass slides by treatment with 300 mJ of UV irradiation followed by 2 h of incubation at 80 °C.