title: SCCPRR_Mouse_19549_v1.1
status: Public on Oct 01 2008
contact.city: Santa Monica
contact.first: Adrian
contact.last: Anghel
contact.email: aanghel@mednet.ucla.edu
contact.state: CA
contact.address: 2825 Santa Monica Blvd. Suite 200
contact.department: Medicine
contact.country: USA
contact.web_link: http://anghel.exofire.net/
contact.institute: University of California, Los Angeles (UCLA)
contact.phone: 310-586-2084
organism: Mus musculus
manufacture_protocol: This array was made using the PGA mouse 70mer oligo library generated at Massachusetts General Hospital. The 19,556 oligos were designed using the OligoPicker software generated at the facility and available for free download at http://pga.mgh.harvard.edu/oligopicker/. Oligos were selected using a stringent set of filters to ensure specific representation of all protein-coding sequences in the NCBI GenPept database. The program designs the oligos to have a Tm within a set 10°C range, to ensure hybridization under similar conditions. Also, OligoPicker avoids sequences that are likely to cross-hybridize with other genes or to self-anneal (form primer dimers). The sequence specificity of each oligo is tested with a global BLAST score and all oligos with a BLAST score above a threshold are discarded. Additional information about the library, including sequences of oligos, is available on the MGH website: https://dnacore.mgh.harvard.edu/microarray/index.shtml.