title: HIS/SIBS Human 15K cDNA array
status: Public on Dec 30 2010
contact.city: Shanghai
contact.first: wentao
contact.last: yang
contact.email: wtaoyang@gmail.com
contact.address: chong qing nan lu #225
contact.department: Institute of Health Science
contact.country: China
contact.institute: Shanghai Institute for Biological Sciences,China Academy of Science
organism: Homo sapiens
manufacture_protocol: 1. cDNA are arrayed in Greiner 384-well Microplates(cat# 784101). Each well contains 50-100 ng/ul cDNA .
  2. Incubate at RT for 30 minutes to aid resuspension of cDNA.
  3. Shake plates near maximum rotational speed on flat-bed shaker for 1 minute.
  4. Centrifuge plates at 2000 rpm for 3 minutes.
  5. Remove seals and cover with plate lids. Place in appropriate location of plate cassette. This should be done with first plates just before print run is started to minimize evaporation time before printing.
  6. Make sure plates rest behind both holding clips in the cassettes. Push plates back into the cassettes as far as they will go, putting them in the proper position for the server arm.
  7. After the print run is completed,the chips are ready for hybridization.