title: 44K multiplex chip, Agilent (Tailor-made microarray, condensed version)
status: Public on May 15 2010
contact.city: Straubing
contact.first: Jochen
contact.last: Schmid
contact.email: j.schmid@tum.de
contact.address: Schulgasse 16
contact.department: Chair of Chemistry of Biogenic Resources
contact.country: Germany
contact.institute: Technische Universität München
contact.phone: 09421 187 331
organism: Agroathelia rolfsii
description: Tailor-made microarray (44K multiplex chip, Agilent) was designed by imaGenes (Berlin, Germany) using an in-house developed method for empirical selection of best performing probes for each gene (Pre Selection Strategy). Briefly, up to ten probes were designed for each of the 454 unigenes (60 bp long oligomers). The 244K Agilent test array was hybridized with pooled Cy3-labeled cRNAs gained form EPSmax13 and EPSmin17 cultures and (in average) two of the best performing oligos were selected for each unigene.
  
  Full physical array layout file linked as a supplementary file, GPL10247_44K_Design.txt.
  The oligonucleotide sequences can be found in the GPL10247_44K_Design.txt.gz file.
manufacture_protocol: http://www.chem.agilent.com/Library/usermanuals/Public/G4140-90040_GeneExpression_One-Color_v6.0.pdf