title: VUMC MACF mouse 22K v9
status: Public on Dec 19 2005
contact.city: Amsterdam
contact.first: Daoud
contact.last: Sie
contact.email: d.sie@vumc.nl
contact.address: De Boelelaan 1117
contact.department: Pathology
contact.country: Netherlands
contact.institute: Vrije Universiteit Medical Center
contact.phone: +31 20 4442428
organism: Mus musculus
description: - oligonucleotide sequences are available via www.ensembl.org after agreeing to Compugen's conditions
  - oligonucleotides were mapped by Ensembl to the NCBIm33 version of the mouse genome
  - all plates were printed once (no replicate spots)
manufacture_protocol: The Mouse 8K and Mouse 14K extension oligonucleotide libraries, containing 65-mer oligonucleotides representing 21825 unique genes as designed by Compugen (San Jose, CA, USA), was obtained from Sigma-Genosys (Zwijndrecht, The Netherlands). The oligonucleotides were dissolved at 10 μM concentration in 150 mM sodium phosphate buffer pH 8.5 and single spotted onto CodeLink™ slides (Amersham Biosciences), using an SA-72 microarrayer (Perkin Elmer) equipped with SMP3 pins (TeleChem International, Sunnyvale, CA, USA). After printing slides were processed and blocked prior to use according to the manufacturer’s protocol.