title: YCU Human 4.2K version 1
status: Public on May 11 2008
contact.city: Yokahama
contact.first: Takeshi
contact.last: Mizuguchi
contact.email: tmizu@yokohama-cu.ac.jp
contact.address: Fukuura 3-9, Kanazawa-ku
contact.department: Department of Human Genetics
contact.country: Japan
contact.web_link: http://www-user.yokohama-cu.ac.jp/~hygiene/
contact.institute: Yokohama City University
contact.phone: +81-45-787-2606
organism: Homo sapiens
manufacture_protocol: We selected 4,219 'FISHed' BAC/PAC clones using UCSC genome browser [2004 May version (http://genome.ucsc.edu/cgi-bin/hgGateway)], spacing at every 0.75 Mb of the whole human genome. All clones spotted showed a unique signal at the predicted chromosomal location by FISH. BAC/PAC DNA was extracted using an automatic DNA extraction system PI-100 (Kurabo, Osaka, Japan), amplified by two-round DOP PCR. DOP-PCR was performed to amplify target-clone DNA using three different 1st DOP PCR primers, DOP-A: CCGACTCGAGNNNNNNATGTGG; DOP-B: CCGACTCGAGNN NNNNTTCAC C; DOP-C: CCGACTCGAGNNNNNNAACAGG. The second PCR was performed using the 5'-amino-labeled primer (GGAAACAGCCCGACTCGAG). Successfully amplified PCR products purified and adjusted to the final concentration >1 ug/μl, and spotted in duplicate on CodeLinkTM activated slides (Amersham Biosciences Corp, NJ) by the ink-jet spotting method (Nihon Gaishi, Nagoya, Japan). Each DNA was spot in duplicate on one slide.