title: FAP-Human-4.8K03 ver01
status: Public on Feb 13 2009
contact.city: São Paulo
contact.first: Alex
contact.middle: Fiorini
contact.last: Carvalho
contact.email: alex.carvalho@hcancer.org.br
contact.address: Rua Professor Antonio Prudente, 211
contact.country: Brazil
contact.institute: Fundação Antonio Prudente
contact.phone: 551133415750
organism: Homo sapiens
manufacture_protocol: Bacteria were grown in LB (Luria-Bertani) medium containing ampicillin (250mg/ml). Inserts were amplified by PCR using M13 reverse and forward primers and products were submitted to 1% agarose gel stained in ethidium bromide for quality control. cDNA clones with single amplicons were purified by filtration in Sephadex G50 (GE Healthcare), following the manufacturer’s directions, printed onto Corning glass slides (Corning) and fixed by UV crosslink.