title: PIQOR Custom Ischemia
status: Public on Dec 01 2007
contact.city: Cologne
contact.first: Bernhard
contact.last: Gerstmayer
contact.email: bernhard.gerstmayer@miltenyibiotec.de
contact.address: Stockheimer Weg 1
contact.country: Germany
contact.institute: Miltenyi Biotec GmbH
contact.phone: 0049-221-95048211
organism: Rattus norvegicus
description: The PIQOR Custom Ischemia Microarray comprises 737 immune related rat and mouse specific cDNAs. These cDNAs include 5 house-keeping genes as positive controls. In addition 4 E. coli DNA fragments and the corresponding in vitro transcribed RNAs (spiking controls) provide a control for the labeling and hybridisation efficiency. 2 negative controls are given by spotted herring sperm DNA and buffer. Spotting in quadruplicate results in 2948 spots exclusive of the spots for the negative controls and the E. coli positive controls.
manufacture_protocol: Defined 200-400 bp fragments of selected cDNAs were generated by RT-PCR, cloned and sequence-verified. Amplified and purified cDNA inserts were spotted in quadruplicate on PIQOR slides using a proprietary piezoelectric spotter. The PIQOR slides were coated in a 2 step process generating a surface which covalently binds DNA molecules.