title: SCKCEN Cupriavidus metallidurans CH34 Genomic Array
status: Public on Mar 16 2007
contact.city: MOL
contact.first: Natalie
contact.last: Leys
contact.email: nleys@sckcen.be
contact.state: Please select one below:
contact.address: 200, boeretang
contact.department: Institute for Environment, Health & Safety
contact.country: Belgium
contact.institute: SCK.CEN
contact.phone: 003214332726
organism: Cupriavidus metallidurans CH34
manufacture_protocol: 1.  Oligos are synthsized and arrayed in Greiner 96-deep well plates. Each well contains more than 10nmol of 60-mer oligo and are resuspended in water to10µM.
  2. Resuspended oligos in water to 10 uM are rearrayed into 384-well (10µl transfered from 96 to 384 well plates), Genetix polystyrene V-bottom plates (cat# X6004).
  5. 10µl of 2x spotting bufferis added to each well of the 384 plate, these plate are considered as spotting master plates
  7. Shake plates near maximum rotational speed on flat-bed shaker for 10 minutes.
  8. Centrifuge plates at 2000 rpm for 3 minutes prior to spotting.
  9. Remove seals and cover with plate lids. Place in appropriate location (biobank in MicroGrid spotting robot) just before print run is started to minimize evaporation time before printing.
  11. After the print run is completed, allow plates to dry through passive evaporation in a protected environment.