title: MPIMG Homo sapiens 44K ArrayCGH4
status: Public on Apr 23 2007
contact.city: Berlin
contact.first: Fikret
contact.last: Erdogan
contact.email: erdogan@molgen.mpg.de
contact.address: Ihnestr. 73
contact.country: Germany
contact.institute: Max-Planck-Institute
organism: Homo sapiens
manufacture_protocol: The submegabase resolution whole genome tiling path BAC array consists of the human 32k BAC Re-Array Set* (BACPAC Resources Center, DNA kindly provided by Nigel Carter, Wellcome Trust Sanger Centre) and a set if subtelomeric clones (assembled by members of the COSTB19 Action: Molecular cytogenetics of solid tumours). Protocols for the isolation of DNA (1 Mb Sanger set and subtelomeric set) and the amplification of insert DNA by linker adapter PCR are described in detail at the Molecular Cytogenetics website of MPG. Amplicons were ethanol precipitated, dissolved in 3XSSC/1,5M betaine and spotted on epoxy-coated slides (NUNC, Wiesbaden, FRG) by means of a modified Qarray (originally from Genetix, new Milton, U.K.) and Pointech (Gibbon,MN) Tungston PTL 2500 slit pins. Each BAC is represented by a single spot on the array. * due to recurrent abnormal ratios in a series of control hybridisation encompassing 30 tumors, clones from plate HGP DNA RP C 12_2 were excluded from further analysis.