title: NKI-CMF Mus musculus 32k oligo array with DamID annotations
status: Public on Jul 28 2006
contact.city: Amsterdam
contact.first: Daoud
contact.last: Sie
contact.email: d.sie@nki.nl
contact.address: Plesmanlaan 161
contact.country: Netherlands
contact.institute: Dutch Cancer Institute
organism: Mus musculus
description: This platform is identical to GPL3675. Due to application in DamID experiments custom annotations are provided. The annotations are derived from blast analysis of the oligo sequence to the genomic sequence. The EnsEMBL release used for retrieving gene, transcript and exon annotations is v33.34. The assembly build that is used as genomic sequence is NCBIM34.
manufacture_protocol: The Mouse Genome Oligo Set Version 3.0 arrays contain 31,769 probes representing 24,878 genes and 32,829 gene transcripts. All oligos are 70-mer sequences. The arrays consist of 37632 features (oligos and controls) and are printed in a 28x28 subarray layout using 48 Biorobotic 10K-microspot pins. We use 14 different Bacillus subtilus sequences as spike-controls printed in triplo in every subarray.