title: Applied Biosystems Customized TaqMan Low Density Array (Mouse) _ In vivo T cell gene expression
status: Public on Jun 27 2008
contact.city: Birmingham
contact.first: Karine
contact.last: Serre
contact.email: k.serre@bham.ac.uk
contact.state: West Midland
contact.address: Wolfson drive, Edgbaston
contact.department: Infection and Immunity
contact.country: United Kingdom
contact.institute: University of Birmingham
contact.phone: 0121 414 6970
organism: Mus musculus
description: To probe T cell responses in vivo, naïve OVA-specific transgenic CD4 T (OTII) cells were transferred into normal mice and the chimeras were then immunized with specific forms of OVA driving either a Th1 or a Th2 type of immune responses. This was achieved respectively by following the response in the draining lymph node after immunizing subcutaneously, either with live attenuated Salmonella typhimurium engineered to express an OVA transgene, or alum-precipitated OVA.
  Virtual platform listing 384 in vivo T cell polarization-related candidate genes investigated using RT-PCR.