title: yeast intergenic array
status: Public on Nov 10 2004
contact.city: Geneva
contact.first: Stephan
contact.middle: B.
contact.last: Schawalder
contact.email: stephan.schawalder@molbio.unige.ch
contact.address: Quai Ansermet 30
contact.department: Departement of Molecular biology
contact.country: Switzerland
contact.institute: University of Geneva
contact.phone: +41 22 379 61 77
organism: Saccharomyces cerevisiae
description: -Platform: amino-silane coated glass slides (GAPS II, Corning)
  
  -S. cerevisiae intergenic regions amplified from S288C genomic DNA (ResGen) using the intergenic region primer oligonucleotides (ResGen) (Harismendy et al. EMBO J. 22(18): 4738-4747, 2003). The primers allow the amplification of the sequence located on either side of elements such as open reading frames, tRNAs, small nuclear RNAs, Ty elements, solo δ, etc. A complete description of the primers can be obtained on the web site (ftp://ftp.resgen.com/pub/genepairs/yeast_intergenic).
  
  -The PCR products were purified and spotted using an automated arrayer (MicroGrid II, BioRobotics)