title: Melitaea cinxia 4x44K v1.0 (Feature number version)
status: Public on Jan 01 2014
contact.city: Helsinki
contact.first: Jouni
contact.middle: Antero
contact.last: Kvist
contact.state: Uusimaa
contact.address: Haartmaninkatu 8
contact.department: Research Program for Molecular Neurology
contact.country: Finland
contact.institute: University of Helsinki
organism: Melitaea cinxia
description: Normalized cDNA from genetically diverse pools of larvae, pupae, and adults body parts, across multiple local populations in the Åland islands were used to de novo assemble a partial transcriptome of the Glanville Fritillary butterfly using 454 pyrosequencing. Probes were designed using Agilent eArray based on the assembled contigs and singletons (version 1). In addition some Sanger sequences and known genes were included in the probe design. For most transcripts a single probe was printed on the array in triplicate. Some probes were also designed to detect possible SNP and splice variants.
  Additional 454-pyrosequencing was conducted after the microarrays were designed. The new EST data was used to re-assemble and re-annotate the transcripts. The probes were matched to the new contigs and singletons (version 2) by BLASTn searches, which were then used to re-annotate the probes by BLASTx searches to other insect species.
  
  Assembly sequences and additional information can be obtained from cinxiabase (http://cinxiabase.vmhost.psu.edu/)
manufacture_protocol: see manufacturer's web site at http://www.agilent.com/