title: Hofmann_UCSB_Mytilus_2.5K_v1.0
status: Public on Mar 26 2008
contact.city: Columbia
contact.first: Sean
contact.middle: P
contact.last: Place
contact.email: places@mailbox.sc.edu
contact.state: SC
contact.address: 715 Sumter St.
contact.department: Biological Sciences
contact.country: USA
contact.institute: University of South Carolina
organism: Mytilus californianus
manufacture_protocol: 1. Samples are provided in 384-well plates with matching lids (Genetix part # X7022) at a concentration of 0.15 µg/µl for PCR products.
  2. Samples must be in a starting volume of 15 µl of spotting buffer (1.5M Betaine, 3xSSC for Corning UltraGaps slides).
  3. cDNAs are spotted on Corning ULTRAGaps (25 mm x 75 mm) using the Biorobotics Microgrid II.
  4. printed slides are held in a desiccator for 24-48 hours after printing and X-linked at 225 mJ for cDNA/PCR products.