title: Agilent-018261 Coupland Lab (MPIPZ)-Arabidopsis/Arabis-244K
status: Public on May 27 2013
contact.city: Cologne
contact.first: Emiel
contact.last: Ver Loren van Themaat
contact.email: themaat@mpiz-koeln.mpg.de
contact.address: Carl von linneweg
contact.country: Germany
contact.institute: Max Planck Institute
organism: Arabidopsis thaliana
description: Custom Arabidopsis array for heterologuos hybridization studies.
manufacture_protocol: Oligos were designed using the eArray application based on the Arabidopsis DNA sequence starting from the less conserved 3´ end and going in the direction of the 5´ end of every gene. Up to 10 oligos / Arabidopsis gene were designed against the DNA sequence (oligo-set). Total of 244K array. The calculated median distance between the end and the start of two consecutive oligos was 27 nt. In situ synthesized 60 mer long oligoes according to stadard Agilent manufacture protocol.