title: Human Promotor Array, v11
status: Public on Mar 23 2004
contact.city: San Diego
contact.first: Yipeng
contact.last: Wang
contact.email: ywang@sdibr.org
contact.state: CA
contact.country: USA
contact.institute: Vaccine Research Institute of San Diego
contact.phone: 8585813960
organism: Homo sapiens
description: Human promoter array. Human promoter sequences (1000bp upstream and 500bp downstream from transcription initiation site) were retrieved batch-wise from GoldenPath. PCR primers were selected using an improved version of Primer3 running in-house on a 24 node Linux cluster. Promoter fragments with an average length of 1.2 kb were amplified, purified and spotted onto UltraGAPS coated slides (Corning Incorporated, Corning, NY) in the presence of 50% DMSO.  A promoter microarray containing triplicate spots of 3083 promoter sequences and 787 non-promoter controls and 192 non-human controls. Many of the promoters on the array are from genes of particular relevance to cancer and the array includes promoters from most of the genes that are known to be regulated by methylation in cancer.
  Keywords = human promoter array