title: JCVI PFGRC Mycobacterium smegmatis 22K v3 array designed primarily based on strain MC2 155 [ORF-centered version]
status: Public on Jul 15 2011
contact.city: St Louis
contact.first: Christina
contact.middle: Leigh
contact.last: Stallings
contact.state: Mo
contact.address: 660 South Euclid Avenue, Box 8230
contact.department: Molecular Microbiology
contact.country: USA
contact.institute: Washington University School of Medicine
organism: Mycolicibacterium smegmatis
description: This is an ORF-centered version of the GEO Platform GPL5862
manufacture_protocol: 4QMS, with arrayer GENETIX, machine cycles 0, pen id 0, pen cycles 0, operator 0, humidity control 0, humidity set point0.000
  Slides were cross-linked in a Stratalinker (Stratagene), using the “auto-link” function at 3000ujoulesx100. Prior to hybridization, slides were pre-hybridized for 2 hours at room temperature in 0.2% SDS then rinsed in filtered water (10 sec) then in 70%.EtOH (5sec). Slides were dried by centrifugation at 1000rpm for 3 min.