title: MPB Murine 16K BMAP-MSV printrun 7.1
status: Public on Jul 01 2011
contact.city: Winnipeg
contact.first: Rhiannon
contact.middle: LCH
contact.last: Huzarewich
contact.email: rhiannon_huzarewich@phac-aspc.gc.ca
contact.state: MB
contact.address: 1015 Arlington St.
contact.department: Molecular PathoBiology
contact.country: Canada
contact.institute: Public Health Agency of Canada
contact.phone: 2047847542
organism: Mus musculus
manufacture_protocol: Arrays were prepared in house from two individual libraries, the Brain Molecular Anatomy Project (BMAP) library and the Mouse Sequence Verified (MSV) Unigene library. There were a total of ~17,000 individual spots on the array including control elements. Inserts from the two libraries was amplified by PCR, and the amplicons were subsequently purified using Millipore multiwell purification plates. The lyophilized PCR products were resuspended in 1x Microspotting Solution Plus (Telechem) to a final concentration of 0.25-0.75 µg/µl. The DNA amplicons were spotted onto CMT-GAPS-coated glass slides (Corning, NY, Cat. 2549) using the Stealth micro-spotting pins (Telechem).