title: Sanger/Nimblegen Human array painting array_Case 1
status: Public on Nov 01 2006
contact.city: Hinxton
contact.first: Nigel
contact.middle: Paul
contact.last: Carter
contact.email: npc@sanger.ac.uk
contact.state: Cambirdge
contact.address: Wellcome Trust Genome Campus
contact.department: Molecular Cytogenetics
contact.country: United Kingdom
contact.institute: Wellcome Trust Sanger Institute
contact.phone: +44 1223 494860
organism: Homo sapiens
manufacture_protocol: Breakpoint spanning oligonucleotide arrays were designed essentially as described previously (Selzer et al., 2005).   Briefly, the regions of interest were repeat-masked.  The array design  contained both 50-mer fixed length probes and isothermal probes that ranged in length from 45 bp to 77 bp. Probes were generated from both strands, and replicated on the array 3 times. The arrays were constructed by maskless array synthesis technology (NimbleGen Systems Inc.), with up to 385,000 oligonucleotides being synthesized by photolithography.
  
  Selzer, R. R. et al. Analysis of chromosome breakpoints in neuroblastoma at sub-kilobase resolution using fine-tiling oligonucleotide array CGH. Genes Chromosomes Cancer 44, 305-19 (2005).