title: BTI DNA microarray for Actinosynema pretiosum
status: Public on Dec 01 2008
contact.city: Singapore
contact.first: Peter
contact.last: Nissom
contact.email: pnissom@gmail.com
contact.address: 20 Biopolis Way
contact.country: Singapore
contact.institute: Bioprocessing Technology Institute
organism: Actinosynnema pretiosum
manufacture_protocol: 1.PCR products were obtained from PCR reactions on genomic DNA of A. pretiosum. The primers were designed based on DNA sequences, AF453501 and U33059 as discussed in Yu et. al., 2002, PNAS, 99:7968. The DNA were arrayed in Abgene 384-well plates. Each well contains 100-150 ng/ul of DNA.
  2. Rearray 10 µL into 384-well, Abgene 384-well microarray plates (AB-1056/w).
  3. Print arrays using Biorad chipwriter pro, after print run is completed, let slides dry through passive evaporation in the arrayer.