title: Nimblegen custom Haemophilus influenzae multi-genome array
status: Public on Jan 21 2009
contact.city: Oklahoma City
contact.first: Timothy
contact.middle: M
contact.last: VanWagoner
contact.email: timothy-vanwagoner@ouhsc.edu
contact.state: OK
contact.address: 940 SL Young Blvd, BMS340
contact.department: Pediatrics
contact.country: USA
contact.institute: University of Oklahoma Health Sciences Center
contact.phone: 405-271-6451
organism: Haemophilus influenzae
description: The Platform data table is a condensed representation of the array's replicate features.  The full array layout representing all of the individual features is linked as a supplementary file at the foot of this record.  PROBE_SEQUENCE information can be found within the full array layout file.
manufacture_protocol: Oligonucleotide arrays were synthesized by NimbleGen Systems, Inc by maskless array synthesis using a digital micromirror array-mediated, parallel synthesis process incorporating 5’-photoprotected phosphoramidites as previously described. (1) Synthesized arrays were deprotected by incubation in ethanolamine:ethanol (1:1) at room temperature for two hours, washed extensively in distilled water, and stored desiccated until used.
  Expression design for 4 strains of H. influenza with 13 probes/gene.  Expression probe set replicated 3x on chip.  All intergenic regions tiled on both strands at a 15bp spacing