title: NIEHS Human Operon Oligo/ToxChip Hybrid V 2.0
status: Public on Nov 02 2005
contact.city: RTP
contact.first: Pierre
contact.middle: Robert
contact.last: Bushel
contact.email: bushel@niehs.nih.gov
contact.state: NC
contact.address: P.O. Box 12233
contact.department: Biostatistics and Computational Biology Branch
contact.country: USA
contact.institute: NIEHS
contact.phone: 919-316-4564
organism: Homo sapiens
description: This array was developed in-house at NIEHS, and used for gene expression profiling experiments. A complete listing of the genes on this chip is available at the following Web site: http://dir.niehs.nih.gov/microarray/chips.htm
  Oligos for spotting were purchased from the Operon Human Genome Oligo Set V1.1 and cDNAs were prepared according to DeRisi et al., 1996, Nat. Genet. 14, 457-460. The  PCR products from the cDNAs and the oligos were spotted onto poly-L-lysine-coated glass slides using a modified, robotic DNA arrayer (Beecher Instruments, Bethesda MD).
manufacture_protocol: http://dir.niehs.nih.gov/microarray/methods.htm