title: YZU_E.Coli_152_V1.0
status: Public on Jun 19 2008
contact.city: yangzhou
contact.first: zhao
contact.last: lixiang
contact.email: zlxfranklin@tom.com
contact.address: 88 Daxue road
contact.department: verterinary school
contact.country: China
contact.institute: Yanzhou university
organism: Escherichia coli
manufacture_protocol: The confirmed PCR products of those genes were precipitated with isopropanol and redissolved in DNA Spotting Solution (CapitalBio Corp. Beijing, China).  Each gene was printed on amino siland slides in triplicate to facilitate subsequent statistical analysis.  After printing, the slides were baked at 80 °C for 1h and stored dry at room temperature till use.
  Prior to hybridyzation, the slides were rehydrated over 65 °C water for 10 seconds, UV cross-linked at 250 mJ/cm2.  The unimmobilized DNAs were washed off with 0.5% SDS for 15 min at 42 °C and SDS were removed by dipping the slides in anhydrous ethanol for 30 seconds.  The slides were spin-dried at 1,000 rpm for 2 min.