title: Drosophila melanogaster detoxification array
status: Public on Jun 16 2006
contact.city: Sophia-Antipolis
contact.first: Gaelle
contact.last: LE GOFF
contact.email: legoff@antibes.inra.fr
contact.address: 400 route des Chappes
contact.country: France
contact.institute: INRA
contact.phone: 33 4 92 38 65 78
organism: Drosophila melanogaster
description: This “Drosophila toxicology microarray” contained 319 genes that represent all cytochromes P450, glutathione S-transferases, esterases, other genes of interest including attacin or Ftz-F1 and several housekeeping genes as controls.
manufacture_protocol: A cDNA microarray was constructed using expressed sequence tags (ESTs) of 1.8 kb average length and gene specific tags of 0.4 kb average length obtained by PCR amplification and plasmid cloning. Each insert was amplified by PCR with flanking universal primers, purified and then spotted using the ChipWriterPro Virtek (BioRad) spotter at three independent locations on Corning UltraGAPS slides (Corning)