title: Agilent-033023 Hzea_Virus_2directions [Feature Number Version]
status: Public on Feb 29 2012
contact.city: Brisbane
contact.first: Quan
contact.middle: Hoang
contact.last: Nguyen
contact.email: quan.nguyen@uq.edu.au
contact.state: Queensland
contact.address: Queensland Bioscience Precinct (Building 80) The University of Queensland. 306 Carmody Road. St
contact.department: Australian Institute for Bioengineering and Nanotechnology
contact.country: Australia
contact.institute: The University of Queensland
contact.phone: (+61)733463147
organism: Helicoverpa zea
description: 29,586 H.zea transcript sequences obtained from denovo assembly were reverse transcribed and then combined with the original sequences to form a final target sequence file that contains 59,172 sequences. Based on these 59,172 sequences, eArray was used to design 3 probes per target, with the options for best distribution method and no 3' bias. Then, all probes with potential cross hybridization were removed, resulting in 153,492 probes. This probe set was divided into 3 probe groups (eArray probe format: complete, Ms-excel), each contains 60,000, 60,000 and 33,000 probes.  Another probe set for 135 virus genes and 16 selected insect genes (3 probes/target), which were replicated 10 times. These four probe groups were then used to design the 4x180,000 Agilent arrays.
manufacture_protocol: see manufacturer's web site at http://www.agilent.com/