title: University of Pisa Dugesia japonica Dj600-chip
status: Public on Jul 19 2006
contact.city: Pisa
contact.first: Leonardo
contact.last: Rossi
contact.email: leoros@biomed.unipi.it
contact.address: Via Volta 4
contact.department: Morfologia Umana e Biologia Applicata
contact.country: Italy
contact.institute: University of Pisa
organism: Dugesia japonica
manufacture_protocol: 612 gene specific 60 mer sense oligonucleotides (Tm from 79 to 88) were synthesized by Invitrogen. Oligonucleotides were re-suspended in 3XSSC at the concentration of 0.5 μg/μl (25 μM) and spotted onto poly-D-lysine coated slides with a configuration of 4 x 13 x 13 using OmniGrid robotic printer (Genemachine). Printed slides were kept in a desiccator at room temperature for 15 days. After UV cross-linking, slides were blocked with succinic anhydride for 15 minutes rinsed in distilled water and denatured in boiling water for 2 minutes. After quick dehydrate in 95% ethanol, slides were dried by centrifugation at 800 rpm for 3 minutes and stored in a desiccators until use.