title: Schistosoma japonicum gender-associated differentially expressed gene array
status: Public on Mar 05 2012
contact.city: Shanghai
contact.first: Yanxun
contact.last: Xia
contact.email: yangmaotian888@yahoo.com.cn
contact.address: 3 Lane 345,Shilong Road
contact.country: China
contact.institute: Shanghai Institute of Domestic Animal Parasitology,Chinese Academy of Agricultural Sciences
contact.phone: 021-54082675
organism: Schistosoma japonicum
manufacture_protocol: The PCR products of cDNA were amplified by use of the polymerase chain reaction (PCR) and then purified with isopropanol precipitation. All PCR products were examined by agarose gel electrophoresis to ensure the quality and the identity of the amplified clones as expected. Then the amplified PCR products were dissolved in a buffer containing 3X SSC solution. The solution with amplified PCR products were spotted onto silylated slides(Biostar, Shanghai, China) with a Omnigrid Arrayer(Gene Machine,CA) fitted with 16 Major Precision split pins.