title: SuperArray GEArray Q series Mouse cAMP/Ca2+ PathwayFinder Gene Array
status: Public on Apr 09 2004
contact.city: Frederick
contact.first: SuperArray
contact.last: Bioscience Corporation
contact.email: support@superarray.net
contact.state: MD
contact.address: Suite 101, 7320 Executive Way
contact.country: USA
contact.institute: Technical Support
contact.phone: 301-682-9200
organism: Mus musculus
description: GEArray Q series Mouse cAMP/Ca2+ PathwayFinder Gene Array contains 96 target genes that are responsive to cAMP or Ca2+. cAMP and Ca2+ are two universal second messengers that mediate a staggering array of different functions, through signaling within the cytoplasm and to the nucleus for activation of gene expression. Both cAMP and Ca2+ signaling converge on CREB, activation of which subsequently affects target genes containing the CRE enhancer element in their promoters. Besides CRE, Ca2+ signaling can also activate target genes with an SRE enhancer element or other, as yet undefined Ca2+ responsive element in their promoters. In addition, cAMP and Ca2+ signals generated from each specific stimuli can vary on concentration, spatial location and duration and can trigger very different transcriptional responses. Therefore, a specific profile of target gene expression can be derived for each stimuli and the profile can be used as a fingerprint to screen for agonists and antagonists for its stimuli. cAMP/Ca2+ PathwayFinder GEArray Q contains three groups of Ca2+ responsive target genes: 1) target genes with SRE; 2) target genes with CRE; 3) target genes with undefined elements. This GEArray is designed for you to distinguish cAMP signaling (only genes in group 1 are activated), and Ca2+ signaling (genes in group 1, 2, 3 are activated), and to study transcription response associated with cAMP and Ca2+ signaling. Through a simple side-by-side hybridization, you can determine simultaneously the change of the expression profile of these 96 genes involved in cAMP/Ca2+ signaling by using your experimental and control RNA samples, arrays and reagents provided in the kit.
  Catalog number MM-028 (Superarray, Frederick, MD)
  Platform_weblink: http://www.superarray.com/home.php
manufacture_protocol: see manufacturer's web site