title: NIAID Pfab
status: Public on Feb 10 2005
contact.city: Bethesda
contact.first: Timothy
contact.middle: G
contact.last: Myers
contact.email: tgm@nih.gov
contact.state: MD
contact.address: 50 South Drive, Room 5509
contact.department: Research Technologies Branch
contact.country: USA
contact.institute: National Institute of Allergy and Infectious Diseases
organism: Plasmodium falciparum
description: Printing by NIAID / Microarray Research Facility
manufacture_protocol: Microarrays are printed with an Omnigrid robotic arrayer by Genomic Solutions that is fitted with a printhead and 48 SMP3 print tips from Telechem.
  Oligonucleotide probes are distributed into 384-well polypropylene plates (Genetix, 384 micro array) and dissolved at 25um in print buffer (150mM sodium phosphate, pH 8.5, 4% sodium sulfate, 0.0001% N-lauryl sarcosyl) .
  The Pfab microarray printing configuration are 4x12, total 6804 oligos are replicated in 48 sub-arrays at 342 spots per sub array wit 18x19 format. Spot densities are 100 Microns, with dot spacing 220 micron (center-to-center).
  The microarrays are printed on Epoxy Slides from Corning (Catalog # 40042) and treated by a method that is an adaptation of the manufacturer's instructions to allow higher throughput processing.