title: Mermaid_Human_32K_CGH_v1.0
status: Public on May 14 2008
contact.city: London
contact.first: Christopher
contact.last: Jones
contact.email: c.jones@ucl.ac.uk
contact.address: Paul O'Gorman Building, 72 Huntley Street
contact.department: Gynaecological Oncology
contact.country: United Kingdom
contact.institute: UCL EGA Institute for Women's Health
contact.phone: 44 2076799204
organism: Homo sapiens
manufacture_protocol: BAC miniprep DNA from the entire 32K BAC clone set was sourced from CHORI. 1µl of CHORI-prepared BAC DNA was amplified with Genomiphi (GE Healthcare) in a 10µl reaction. Amplified DNA was purified using the Millipore PCR384 cleanup plates and resuspended in 30µl Genetix print buffer. All reactions were set up using Biomek2000 liquid-handling robotics in barcoded microtitre plates and tracked through the manufacturing process using a bespoke LIMS database.
  Arrays were printed onto Corning Ultragaps slides with a modified Genetix QArray2 printing robot, equipped with a Parallel Synthesis print head and 48x  50µm Parallel Synthesis silicon pins. Humidity of 65% was maintained throughout printing, and other printing conditions were optimized to provide average feature diameters of approximately 100µm ±10µm. Printing was performed in a climate controlled, positive pressure, HEPA/activated charcoal filtered laboratory.