title: V6.1 BAC-Emulation Oligonucleotide Array
status: Public on Aug 17 2010
contact.city: Houston
contact.first: zhishuo
contact.last: ou
contact.email: zhishuoo@bcm.edu
contact.state: TX
contact.address: One Baylor Plaza
contact.department: Molecular and Human Genetics
contact.country: USA
contact.institute: Baylor College of medicine
contact.phone: 7137984365
organism: Homo sapiens
manufacture_protocol: See manufacturer's web site
  The BAC emulated Version 6.1 OLIGO array was comprised of approximately 42,460 oligonucleotides representing 1400 BAC clones, covering ~150 genomic disorders, all 41 subtelomeric regions up to 12 Mb and 43 pericentromeric regions with backbone coverage of every chromosome at the 650-band level of cytogenetic resolution (http://www.bcm.edu/geneticlabs/cma/tables.html). The 42.46K oligonucleotides (oligos) were selected from initial testing of 105,000 oligos derived from the Agilent eArray library with strict selection criteria and removal of repetitive sequences to ensure optimal performance with greater dynamic range. This targeted 42.46 K OLIGO array (V6 OLIGO) corresponds to genomic regions covered by the V6 BAC arrays and was manufactured in a 4 x 44K format with an average of 28-30 oligos per region previously covered by a single BAC clone.