title: UCSF/Sil lab-Histoplasma capsulatum-G217Borfs3
status: Public on Aug 12 2013
contact.city: San Francisco
contact.first: Mark
contact.last: Voorhies
contact.email: mark.voorhies@ucsf.edu
contact.state: CA
contact.address: 513 Parnassus, S472
contact.department: Microbiology and Immunology
contact.country: USA
contact.institute: University of California
organism: Histoplasma capsulatum
description: This is a revision of UCSF/Sil lab-Histoplasma capsulatum-G217Borfs2 (GEO accession GPL14901) adding 269 oligos and removing 425. All removed oligos correspond to spike-in controls in the original design. The new oligo IDs are prefixed with G217Borfs3 or G217Borfs4 and correspond to putative novel transcripts from two stages of analysis of whole genome tiling array data (published with a revised and expanded set of novel transcripts in BMC Microbiol. 11:216).  HistoBase-compatible genome coordinates for the targets of the new oligos are given in the Target column.  This platform differs from UCSF/Sil lab-Histoplasma capsulatum-G217Borfs4 (GPL17161) in the physical location of the spotted oligos, including a partial, redundant printing of a subset of oligos at the bottom of the array (the redundant spots are labeled as G217Borfs1.14*.Misprint).
manufacture_protocol: Spotted using DeRisi arrayer in Center for Advanced Technology, Genentech Hall, Mission Bay.  70-mer oligonucleotides representing each predicted gene are resuspended in 3XSSC and spotted on poly-lysine slides purchased from Erie Scientific.