title: febit Homo sapiens miRBase 16.0
status: Public on Jun 20 2012
contact.city: Bad Krozingen
contact.first: Thomas
contact.middle: G
contact.last: Nuehrenberg
contact.email: tnuehrenberg@gmail.com
contact.state: Baden-Württemberg
contact.address: Südring 15
contact.department: Klinik für Kardiologie II
contact.country: Germany
contact.institute: Universitäts-Herzzentrum Freiburg • Bad Krozingen
contact.phone: +49-7633-402-0
organism: Homo sapiens
description: Geniom Biochip MPEA homo sapiens. The array is built on the miRNA base 16.0
  in-situ oligonucleotides - additional nucleotides are bound on the 5'end of each capture oligonucleotide necessary for the enzymatic extention in the labeling procedure. The probes are synthesized with intra-array replicates to increase the statistical confidence and to compensate for potential positional effects. As a result the raw data files contain a total of 11 data points for each miRNA. The intensities of blank probes which consist only of one single T nucleotide are used for background corrections. Spike-in controls for the labeling effciency are also present. In order to have a grip on the hybridization process as well as positioning features, additional hybridization controls are added to the array template. Blank, labeling control and hybridization control probes are not included in the data analysis.
manufacture_protocol: MicroRNAs (miRNAs) are endogenous, non-coding RNAs that play a fundamental role in gene regulation and have critical functions across various biological processes. Pairing to mRNAs of protein-coding genes, they direct posttranscriptional repression by altering the stability or translational efficiency of their target mRNAs. Recent studies indicate that miRNAs are involved in the development of human malignancies, suggesting that they represent a promising new class of cancer biomarkers. Identifying miRNAs involved in specific cancers could provide useful diagnostic information, allow disease classification and reveal potential treatment targets. MPEA: On-Chip Labeling for Highest Sensitivity Enzymatic labeling assay requires minimal sample amount In addition to the standard hybridization of pre-labeled RNA, the microfluidic Geniom Biochip enables a highly sensitive enzymatic labeling protocol.The unique Microfluidic Primer Extension Assay (MPEA) uses unlabeled miRNAs for hybridization. In a second step, Klenow fragment of DNA polymerase I is added directly into the channels of the microfluidic chip, where the specific elongation of the bound miRNAs takes place. The method combines the specificity of a hybridization assay with the discriminatory power of an enzymatic extension and reduces the required sample amount to a minimum.