title: Paro_D.melanogaster_0.9k_v4
status: Public on Sep 25 2007
contact.city: Basel
contact.first: Christian
contact.last: Beisel
contact.email: christian.beisel@bsse.ethz.ch
contact.address: Mattenstrasse 26
contact.department: Biosystems Science and Engineering
contact.country: Switzerland
contact.institute: ETH Zurich
contact.phone: +41 61 387 31 65
organism: Drosophila melanogaster
description: Nexterion E Slides are epoxy coated
manufacture_protocol: Primers were designed using the GenomePRIDE software (Haas et al., 2003) to amplify 545 fragments representing the Drosophila Antennapedia complex, ANT-C, (AE001572) and 339 fragments covering the Drosophila Bithorax complex, BX-C, (U31961(Martin et al., 1995)). The fragments are about 1 kb in size with 20-200 bp overlap between neighbouring fragments. PCRs, and spotting of the fragments were carried out as described (Hild et al., 2003). PCR products were purified with Nucleofast 96 (Macherey-Nagel) and spotted onto Nexterion E slides (Peqlab). Non-Drosophila cDNAs were spotted as spike-in controls. The array contains 8 spotted replicates of each PCR fragment.
  Haas, S. A., Hild, M., Wright, A. P., Hain, T., Talibi, D., & Vingron, M. (2003) Nucleic Acids Res 31, 5576-5581.
  Martin, C. H., Mayeda, C. A., Davis, C. A., Ericsson, C. L., Knafels, J. D., Mathog, D. R., Celniker, S. E., Lewis, E. B., & Palazzolo, M. J. (1995) Proc Natl Acad Sci U S A 92, 8398-8402.
  Hild, M., Beckmann, B., Haas, S. A., Koch, B., Solovyev, V., Busold, C., Fellenberg, K., Boutros, M., Vingron, M., Sauer, F., et al. (2003) Genome Biol 5, R3.