title: MeLiM_tiling_array_SSC1
status: Public on Jul 05 2019
contact.city: Jouy-en-Josas
contact.first: Sylvain
contact.last: Marthey
contact.email: abridge@inra.fr
contact.address: Domaine de Vilvert Bat 320
contact.department: Animal Genetics
contact.country: France
contact.institute: INRA
organism: Sus scrofa
description: The MeLiM_tiling_array_SSC1  high-density oligonucleotide tiling array was design to cover both strands of the locus from 177500000 bp to 183640000bp on chr1 (Sscrofa v9.2 2009). The tiling array also contained probes covering the exons of the MITF gene, added as a positive control of expression in pig melanoma (Bourneuf et al., 2011). The reference sequences used for the design were retrieved from Ensembl database Sscrofa9.66 release (ftp://ftp.ensembl.org/pub/release-66/fasta/sus_scrofa/) including 6,140,000 nt for the chromosome 1 locus, and 64,400 nt for MITF exons.
manufacture_protocol: Maskless array synthesis was used to construct isothermal arrays. An isothermal format (Tm=76oC) and probe length constraint between 50 and 75 bp were used for probe synthesis. Each probe overlapped its neighbor by about 40 base pairs.