title: NanoString Human Glial Profiling Panel v1.0
status: Public on Jan 12 2022
contact.city: Atlanta
contact.first: Ashlyn
contact.middle: G
contact.last: Johnson
contact.email: ashlyn.grace.johnson@emory.edu, cmhales@emory.edu
contact.state: GA
contact.address: 615 Michael Street NE
contact.department: Neurology
contact.country: USA
contact.institute: Emory University
organism: Homo sapiens
manufacture_protocol: Two probes hybridize directly to a target molecule in solution. The Reporter Probe carries the fluorescent barcode and the Capture Probe contains a biotin moiety that immobilizes the hybridized complex for data collection. After hybridization, samples are transferred to an nCounter instrument which removes excess probes. Purified target-probe complexes are bound, immobilized and aligned on the imaging surface of the nCounter cartridge. Sample cartridges are scanned by an automated fluorescence microscope. Barcodes are counted for each target molecule and the data are exported as a simple CSV file. (https://www.nanostring.com/)