title: NHRI Human 46K cDNA array
status: Public on Jul 30 2009
contact.city: Miaoli County
contact.first: Shu-Chen
contact.last: Liu
contact.email: jennyliu66@gmail.com
contact.address: 35 Keyan Road,Zhunan
contact.country: Taiwan
contact.institute: National Health Research Institutes
organism: Homo sapiens
manufacture_protocol: The NHRI human cDNA microarray contains 46,657 cDNA clones (IMAGE consortium), representing approximately 26000 unique Unigene clusters. We fabricated cDNA microarrays essentially following protocols  as described by Michael B. Eisen and Patrick O. Brown with minor modification. Briefly, the purified cDNA products were resuspended in spotting solution containing 50% dimethyl sulfoxide. The final concentration of each probe was around 0.2 to 0.5 mg/ml. These probes wererobotically arrayed onto aminosilane-coated UltraGAPS glass microarray slides (Corning) using a home-made robotic microarray spotter. In addition to the human cDNA probes, a series of control DNA probes from the Lucidea Universal ScoreCard (Amersham Bioscience) were also included for quality assessment of the microarray experiment. Totally, there are 49152 probes spotted on a single microarray slides.