title: Geobacter sulfurreducens PCA cDNA array (IAS_GGS3)
status: Public on Aug 31 2007
contact.city: Rockville
contact.first: Chun-Hua
contact.last: Wan
contact.email: cwan@jcvi.org
contact.state: MD
contact.address: 9704 Medical Center Dr
contact.department: Pathogen Functional Genomics Resources Center (PFGRC)
contact.country: USA
contact.institute: The J. Craig Venter Institute
contact.phone: 301-795-7707
organism: Geobacter sulfurreducens PCA
description: In the platform data table, the following FEAT_Name values are of controls with additional description:
  FEAT_NAME	Description
  RCP1_AF168390	A. thaliana root cap 1 protein (RCP1)-Control
  rbcL_U91966	A. thaliana ribulose-1,5-bisphosphate (rbcL)-control
  Cab_X56062	A. thaliana chlorophyll a/b-binding protein (Cab)-control
  rca_M86720	A. thaliana RUBISCO activase (RCA)-control
  TIM_AF247559	A. thaliana triosephosphate isomerase (TIM)-Control
  NAC1_AF198054	A. thaliana NAC1 (NAC1)-control
  LTP6_AF159803	A. thaliana lipid transfer protein 6 (LTP6)-control
  LTP4_AF159801	A. thaliana lipid transfer protein 4 (LTP4)-Control
  RPK_X58149	A. thaliana ribulose-5-phosphate kinase (PRK)-control
  XCP2_AF191028	A. thaliana papain-type cysteine endopeptidase (XCP2)-Control
  POSITIVE	Positive Control-mixture of PCR templates
  NEG1_Baculovirus_Vector	Control
  NEG2_Human_Dynein	Control
  
  The Platform data table reflects the condensed array layout. The full array layout representing the individual 6x-spotted features is linked below as a Supplementary file (ie, GPL5768_full_array_layout.txt).
manufacture_protocol: Following amplification and cleanup, amplicons were resuspended to a concentration of 100-200nM in 50% DMSO and printed onto slides using a Brooks Automation Systems (Cambridge, MA) array spotter. All amplicons were spotted six times per slide. Postprinting, all slides were cross-linked using a Stralinker UV cross-linker (Stragene) and stored under vacuum until use.