title: DiSteBa_Homo sapiens_Glioblastoma miRNA 340_v1.0
status: Public on Dec 19 2009
contact.city: Lecce
contact.first: Oscar
contact.middle: Fernando
contact.last: D'Urso
contact.state: Lecce
contact.address: provinciale Monteroni
contact.department: Department of biological science
contact.country: Italy
contact.institute: Salento University
organism: Homo sapiens
description: The substrates used were Nexterion Slide E epoxy slides.
manufacture_protocol: Probe set relative to all miRNA were designed by means of ArrayDesigner4. Probes were purchased dry in 384 well plate and resuspended in 3X SSC as spotting buffer. Spotting was performed by means of SpottArray 428 (Perkin Elmer), under humidity range of 50%-60% with optimum setted to 55%. All probes were spotted in duplicate. After printing procedure slides were incubated in a 95% humidity chamber for 30'. After dry to 60° for 20 minutes slides were stored dry and light protected upon usage.