title: UCT Brombacher lab Mus MEEBO 40K version1
status: Public on Sep 12 2007
contact.city: Cape Town
contact.first: Anita
contact.middle: R
contact.last: Schwegmann
contact.email: SCHANI002@uct.ac.za
contact.state: Western Cape
contact.address: Institute for Infectious Diseases and Molecular Medicine, UCT medical School, Anzio Rd, Observatory
contact.department: Division of Infectious Immunology
contact.country: South Africa
contact.institute: Institute for Infectious Diseases and Molecular Medicine, University of Cape Town
contact.phone: 27214066035
organism: Mus musculus
description: The MEEBO (Mouse Exonic Evidence Based Oligonucleotide) Set contains a collection of oligo probes, largely derived from constitutively expressed exons, allowing interrogation of almost 25,000 mouse genes. The set was designed to enable study of mouse transcription patterns and, as
  broadly as possible, alternative splicing. An exon-centric design was selected to allow the differentiation of constitutively expressed versus alternatively expressed exons. This design also supports comparative genome hybridization (CGH) analysis. In addition to the exon-centric probes, the set contains an extensive assortment of controls that facilitate accurate evaluation of expression results.
  The MEEBO Set contains 38,467 70mer oligonucleotide probes with an amino modification on the 5’ end. Probes are provided at a final yield of 200pmol each, and delivered in 101 384-well microarray print plates (Genetix model X7020). Probes are Tm normalized, and are designed to minimize cross-hybridization and secondary structure.
manufacture_protocol: Platform type: spot printed
  Slide surface: Gamma Amino Propyl Silane (GAPS)
  Slide dimensions: 25 x 75mm
  Spot diameter: 100um from 10K pin set
  Spot attachment: ionic during to printing then covalent attachment via UV-crosslinking and baking
  Printer: MicroGridII, BioRobotics
  Software: TAS, BioRobotics
  Printing solution: 50% DMSO
  Drying after printing: 12 hour minimum, room temp.
  UV crosslinking: 120mJ
  Baking: 2hour 80°C