title: Malaria Print 20060818ML_PMC
status: Public on Dec 11 2008
contact.city: Pretoria
contact.first: Anna
contact.middle: Catharina (Tharina)
contact.last: van Brummelen
contact.email: tvbrummelen@csir.co.za, lbirkholtz@up.ac.za
contact.state: Gauteng
contact.address: Lynwood Road
contact.department: Biochemistry
contact.country: South Africa
contact.institute: University of Pretoria
contact.phone: +27(0)12 420-2479
organism: Plasmodium falciparum
manufacture_protocol: 1. Oligos are arrayed in 384-well Genetix polystyrene V-bottom plates (cat# X7020). Each well contains 600 pmol of 70-mer oligo.
  2. Resuspend oligos in 3X SSC to 40 uM.
  3. Lyophilize oligos in Genetix plates by centrifugation in a Savant Speed Vac plate spinner (Model SC210A).
  4. Before printing, add 15 µL of water to each well. This can be done the night before a print run is started.
  5. Seal plates with parafilm and shake gently overnight at 4C.
  7. Centrifuge plates at 2000 rpm for 1 minute immediately prior to loading on the printer.
  8. Remove parafilm immediately prior to loading ech plate.
  9. Load plate onto platform in order designated, as each round completes
  10. Cover completed plate with parafilm and return to 4C.
  11. After the print run is completed, Speed Vac plates to dry.
  12. For each subsequent preparation of these plates for a print run, add water to the wells. The amount of water should be decreased by 0.5 µL per print run, as this is the amount drawn up by the pin capillary during each print.
  poly-lysine glass from Erie Scientific