title: CombiMatrix_custom design 7327
status: Public on Jun 01 2012
contact.city: Beaverton
contact.first: Maria
contact.last: Smit
contact.email: smitm@ebs.ogi.edu
contact.state: OR
contact.address: 20000 NW Walker Rd
contact.department: Environmental and Biomolecular Systems
contact.country: USA
contact.institute: Oregon Health and Science University
contact.phone: 425-923-6433
organism: Bradyrhizobium
  Rhizobium
  Skeletonema
  Thalassiosira
  Pseudo-nitzschia
  Mesorhizobium
  Roseovarius
  uncultured marine crenarchaeote
  uncultured Pelagibacterales bacterium
manufacture_protocol: The custom oligonucleotide microarray is synthesized on a semiconductor using an electrochemical synthesis process. Each oligonucleotide probe (spot) is synthesized on a platinum electrode that is independently controlled by the synthesizer’s computer. Synthesis is based on established phosphoramidite chemistry and occurs at thousands of sites simultaneously according to a computer algorithm that activates only specified electrodes. Since physical photolithographic masks, mechanical micromirrors, or inkjet technologies are not involved in the process, all probes can be easily changed without extra time or cost.
  The oligonucleotides are synthesized in a proprietary porous reaction layer that coats the surface of the semiconductor, and acts to covalently attach the oligonucleotide to the surface of the chip.