title: salivary gland secretion
status: Public on Nov 05 2006
contact.city: Rochester
contact.first: Alaka 
contact.last: Srivastava
contact.email: Alaka_Srivastava@urmc.rochester.edu
contact.state: NY
contact.address: 601 Elmwood Avenue
contact.department: Oral Biology
contact.country: USA
contact.web_link: http://www.urmc.rochester.edu/dentistry/faculty/srivastava.cfm
contact.institute: University of Rochester Medical Center
contact.phone: 585 273 1424
organism: Homo sapiens
  Mus musculus
description: UltraGap Gamma Amino Propyl Silane slides (Corning, NY)
manufacture_protocol: Probes for target genes were designed to include key water and ion transport proteins, as well as many secretion-associated signaling molecules and representative secretory proteins.  Human and mouse cDNAs representing 188 genes were obtained from either Open Biosystems (Huntsville, AL) or the “Mouse 15K cDNA Clone Set” (National Institute of Aging).  PCR primers were designed to amplify 200-1200 bp products from the 3’ ends of highly homologous regions of the human and mouse genes.  PCR products of the expected length were purified sequence verified, and dried.  Products were resuspended at 200 ng/µl in Pronto printing buffer (Corning Inc., Corning, NY) and printed onto UltraGap Gamma Amino Propyl Silane slides (Corning, NY) using a BioRad VersaArray arrayer and 8 SMP3B stealth pins (TeleChem International Inc., Sunnyvale, CA).  Each cDNA was printed (~125 μm diameter) in duplicate at adjacent sites with spot to spot separation of 375 μm.