title: Agilent-024600/Klug lab Rhodobacter sphaeroides 2.4.1 - 15k array v1 (122 sRNAs)
status: Public on Oct 26 2011
contact.city: Giessen
contact.first: Sebastian
contact.middle: Harald Michael
contact.last: Metz
contact.email: Sebastian.Metz@mikro.bio.uni-giessen.de
contact.address: Heinrich-Buff-Ring 26-32
contact.department: Institut für Mikro- und Molekularbiologie
contact.country: Germany
contact.institute: Justus-Liebig-Universität Giessen
organism: Cereibacter sphaeroides 2.4.1
description: spotted 60 nt oligonucleotides
manufacture_protocol: High-density oligonucleotide R. sphaeroides microarrays (Agilent gene chips corresponding to the whole 4.6-Mb genome, Peuser et al., 2011) were used for transcriptome profiling. The microarray contains probes against 4,304 protein coding genes and, 122 intergenic regions and 79 rRNA and tRNA genes; its construction and performance analysis was performed according to the instructions of Agilent (www.chem.agilent.com). Three antisense probes with a length of 60 nt were designed for hybridization to each gene.