title: UNMC_Homo Sapiens_10K_chipA
status: Public on Aug 01 2007
contact.city: omaha
contact.first: Javeed
contact.last: Iqbal
contact.email: jiqbal@unmc.edu
contact.state: NE
contact.address: 40th street
contact.department: Pathology and Microbiology
contact.country: USA
contact.institute: University of Nebraska Medical Center
contact.phone: 402-559-7753
organism: Homo sapiens
manufacture_protocol: Microarrays with 60-mer oligonucleotides (Compugen, Jamesburg, NJ) printed on lysine-coated glass slides representing 17,260 genes, including reference (control) genes were utilized for hybridization. Compugen oligo Catalog# HUMLIB96: Human OligoLibrary Release 1.0: 96 well format, 197 plates (18,861 oligos).
  Oligonucleotide probes (60mers) were designed for each target gene (Compugen, Inc., Rockville, MD) and manufactured by Sigma-Genosys, Inc. (The Woodlands, TX).  Oligonucleotides (ONs) were resuspended (30µM) in 3X SSC and spotted by the DNA Microarray Facility at the Eppley Cancer Center/Unviersity of Nebraska Medical Center onto poly-L-lysine coated slides using a MagnaSpotter robot (BioAutomation Corp., Dallas, TX) with a 12-pin print head (Telechem, Sunnyvale, CA) configuration in a humidified, HEPA-filtered hood. After spotting, the DNA was cross-linked to the slides by UV irradiation (350mJ/cm2) with a Stratalinker UV Crosslinker (Stratagene, Inc., La Jolla, CA), blocked by succinic anhydride treatment, and rinsed in ethanol.  A total of 18,705 oligonucleotides were spotted representing 17,260 different genes.
  GPL5376 (UNMC_Homo sapiens_10K_chipB) and GPL5377 (UNMC_Homo Sapiens_10K_chipA) together represent the 18,861 oligos in the Compugen oligonucleotide library.