title: GAP_M_I
status: Public on Jan 15 2003
contact.city: Raleigh
contact.first: Ahmad
contact.last: Fakhoury
contact.email: amfakhou@siu.edu
contact.state: NC
contact.department: Plant Pathology
contact.country: USA
contact.institute: North Carolina State University
contact.phone: 919-515-6995
organism: Aspergillus flavus
description: PCR products were generated using approximately 5 ng plasmid DNA isolated from unique cDNA clones. T3 and T7 primers were used to generate amplicons. PCR products were then purified by isopropanol precipation followed by a 70% ethanol wash. The presence of amplicons was verified by agarose gel electrophoresis.
  PCR products at approximately 250 ng/ul in 50% DMSO were spotted in triplicate onto Telechem SuperAldehyde slides (Sunnyvale, CA) using an Affymetrix 417 Arrayer (Santa Clara, CA). Spots were allowed to air dry before being fixed following manufacturer’s recommendation.
  Keywords = aspergillus, aflatoxin