title: Avian intestinal intraepithelial lymphocyte cDNA microarray (AVIELA) version 2
status: Public on Jun 02 2009
contact.city: Beltsville
contact.first: Duk Kyung
contact.last: Kim
contact.email: duk.kyung@gmail.com
contact.state: MD
contact.address: Bldg. 1043, BARC-East
contact.country: USA
contact.institute: USDA, ARS
organism: Gallus gallus
manufacture_protocol: The microarray was constructed from 10,162 spot elements selected from the previously prepared IEL clones (Min et al., 2005) and the immune-related genes from lipopolysaccharide (LPS)-activated macrophage (HD11) cDNA library (Min et al., 2003) as well as the direct PCR clones of several cytokines and chemokines (Min et al., 2005). PCR products of the ESTs were dried and resuspended in 3X SSC, 0.01% SDS, followed by spotting in duplicate  onto MicroMax glass slides (Perkin Elmer, Boston, MA) using an OmniGrid Accent spotter (GeneMachines, San Carlos, CA).  The elements were organized into duplicated 24 subarrays, each as a 21 x 21 spot grid.  The PCR products were fixed by UV cross-linking, followed by assessment of spotting quality by staining with SYBR-Green II according to the manufacturer's protocol (Invitrogen, Carlsbad, CA).  Greater than 90% of the spots possessed an verage diameter of 150-200 µm.