title: LU/IBL_D.rerio_44k_015747
status: Public on Jan 29 2009
contact.city: Neuherberg
contact.first: Katharine
contact.last: Webb
contact.email: katharine.webb@helmholtz-muenchen.de
contact.address: Ingolstaedterlandstr. 1
contact.department: Institute for developmental genetics
contact.country: Germany
contact.institute: Helmholtz Zentrum Muenchen
organism: Danio rerio
description: The micro-array slides were custom designed by Agilent Technologies. The slides contain in total 43.371 probes of a 60 oligonucleotide length. Of these probes a total of 21.496 probes were identical to the probes present on the Agilent probe set that is commercially available under catalog number 013223_D.  Most of the additional probes were designed using the eArray software from Agilent Technologies (https://earray.chem.agilent.com/earray/).  Settings used were based on the following settings: base composition methodology, best probe methodology and design with 3’ bias. The Agilent D.rerio transcriptome was used as a reference database. A small number of probes were manually designed based on knowledge of particular polymorphisms for genes encoding protein families such as 14-3-3 proteins, chitinase-like proteins and Toll-like receptors  in order to obtain gene-specific probes.
manufacture_protocol: the arrays were produced according to manufacturer's protocol, check website: http://www.chem.agilent.com/en-US/products/instruments/dnamicroarrays/pages/default.aspx