title: URGV Arabidopsis thaliana 34.6K CATMA_v5
status: Public on Nov 11 2009
contact.city: evry
contact.first: Soubigou-Taconnat
contact.last: Ludivine
contact.email: soubigou@evry.inra.fr
contact.address: 2 rue gaston crémieux
contact.department: URGV
contact.country: France
contact.institute: INRA
organism: Arabidopsis thaliana
description: Corning Ultra-GAPS (TM) slides are spotted with 64 pins (8x8) therefore the spotted area is divide into 3 metablocks, each containing 64 blocks of 196 spots (14x14).
manufacture_protocol: A.thaliana genes were predicted with Eugene software (Foissac S. et al., Nucleic Acids Res., 2003) and gene-specific sequence tags were automatically designed (Thareau et al. Bioinformatics, 2003 Nov 22). Two rounds of PCR were performed and PCR products were finally purified and dissolved in 50% DMSO in TE at concentrations ranging between 50-200ng/ul and re-arrayed in 1536 microtiter plates. The pins are washed in 0.1% SDS at 50 degre Celsius then three times in bi-distilled water. Spotter settings are 45-55% humidity, and a pitch = 180 um. The room temperature is set between 19 and 21 °s Celsius and below 40 % humidity. The microtiter plates are placed in the biobank. The four trays are cleaned and 100-120 slides placed on them (30 slides per tray). The spotting lasts 25 hours. The slides are stored in the dark in a dry-cabinet for 3 days and UV-cross-linked at 300 mJ. After crosslinking, the slides are stored in the dark in a dry-cabinet.This array design contains:
  - 32626 Gene Specific Tags (GST)
  - 44 Non Protein Coding (NPC)
  - 1263 microRNA precursors.