title: Axtell_Physco_rdr6_Linker3 (Solexa sequencing)
status: Public on Dec 19 2008
contact.city: University Park
contact.first: Michael
contact.middle: J
contact.last: Axtell
contact.email: mja18@psu.edu
contact.state: PA
contact.address: 280 Mueller Lab
contact.department: Biology
contact.country: USA
contact.institute: Pennsylvania State University
organism: Physcomitrium patens
description: Small RNAs were sequenced using a Solexa GA.  Three different linkers were used for different samples and mixed prior to sequencing.  Raw data was filtered to identify small RNAs associated with each linker.  Subsequently, small RNAs were filtered to remove rRNA fragments.  The remainder were mapped to the Physcomitrella patens draft genome version 1.2 (using oligomap) and filtered to retain only those with one or more perfect match.  This platform is all small RNA sequences from two technical replicates from 10 day old rdr6-19 plants.  See Cho et al. for details.