title: Qiagen/Operon version 2.0 mouse oligonucleotide
status: Public on Aug 01 2005
contact.city: Belmont
contact.first: Ting Ting
contact.last: Zhang
contact.email: tizst0@yahoo.com
contact.state: MA
contact.address: 115 Millman st
contact.department: Mclean Hospital
contact.country: USA
contact.institute: Harvard University
contact.phone: 617-855-2179
organism: Mus musculus
manufacture_protocol: The Qiagen/Operon version 2.0 mouse oligonucleotide library, comprised of oligonucleotides (70-mers) representing 16,443 unique mouse genes, was printed on poly-L-lysine-coated glass slides. The library also included sets of positive and negative controls that were used for quality control purposes. Oligonucleotides were prepared in sets of 384-well plates at a concentration of 40 <E6>M. Arrays were prepared using a custom-built array printer equipped with Majer MicroQuill-2000 split tip pins with a capacity of 255 slides per print run. The printed arrays were numbered sequentially during each run and tracked by a unique identification number. After completion of printing and air drying of the arrays, all slides were washed in 0.2% SDS to remove excess oligonucleotide followed by a brief rinse in 70% ethanol. Slides selected from throughout the run were tested for correct printing by nonspecific hybridization of Cy3-labeled, random 9-mers.