title: Human Versarray 4.0
status: Public on Aug 09 2007
contact.city: Heidelberg
contact.first: Cordula
contact.last: Tschuch
contact.email: c.tschuch@dkfz.de
contact.address: Neuenheimer Feld 580
contact.country: Germany
contact.institute: DKFZ
contact.phone: +49-6221-424594
organism: Homo sapiens
manufacture_protocol: Synthetic 70mer oligonucleotides were dissolved in 15ml 3x SSC, 1.5M Betaine spotting buffer to a final concentration of 20 pmol/ml.
  DANN spotting was performed on QMT epoxysilane coated slides in an air-conditioned room at 21°C and 42% relative humidity using a VersArray Chip Writer Pro System equipped with Stealth SMP3 Micro Spotting Pins.
  Spot centers were 155 mm apart with a diameter of around 100mm per spot. The dipping time of the spotting pins was 2s for loading, spotting pins hit the arrays with 5mm/s.
  Spots were printed in parallel using a print head containing 48 print tips. Printing proceeds from top to bottom and left to right in each print tip group.
  The print head takes up template from standard 384-well plates, each individual plate supplying template for 8 consecutive spots in each tip group.
  DNA adhesion to the glass surface was accomplised by 2h incubation at 60°C.