title: FHCRC Human 18K Array
status: Public on Feb 09 2002
contact.city: Seattle
contact.first: Lynn
contact.last: Graf
contact.email: lgraf@fhcrc.org
contact.state: WA
contact.address: 1100 Fairview Ave. N. D1-100
contact.department: Clinical Research Division
contact.country: USA
contact.web_link: http://none
contact.institute: Fred Hutchinson Cancer Research Center
contact.phone: 206-667-4545
organism: Homo sapiens
description: Microarray construction and hybridization protocols were modified from DeRisi, J.L., V.R Iyer, and P.O. Brown. Science 278:680-686, 1997. The microarrays were constructed using a set of more than 17000 sequence-verified clones from Research Genetics (UniGene Build 136). The cDNA inserts were amplified from replica plate cultures by PCR, purified and mechanically spotted onto polylysine-coated microscope slides using an OmniGrid robotic spotter (GeneMachines, San Carlo, CA).