title: University of Minnesota Human miRNA version 2
status: Public on Jul 30 2009
contact.city: Minneapolis
contact.first: Xiaoxiao
contact.last: Zhang
contact.email: zhang688@umn.edu
contact.address: 312 Church St. SE
contact.country: USA
contact.institute: University of Minnesota
organism: Homo sapiens
manufacture_protocol: A miRNA probe set was purchased from Invitrogen, which was designed based on the Sanger miRBase Sequence Database, Release 9.0.  The set contains approximately 1,140 oligonucleotides of 34 to 44 nt long as probes.  They are complementary to C. elegans, Drosophila, zebrafish, mouse, rat, and human miRNAs, and also include a number of internal and negative control probes.  The oligonucleotides were dissolved in 3 x SSC and quadruply printed on Corning® GAPSTM II coated slides  using a BioRobotics Microgrid II spotter