title: University of Alberta Escherichia coli 19K v2.0
status: Public on Jun 30 2011
contact.city: Ann Arbor
contact.first: Dongjuan
contact.last: Dai
contact.email: joandai@umich.edu
contact.state: MI
contact.address: 109 Observatory St
contact.department: Environmental Health Sciences
contact.country: USA
contact.institute: University of Michigan
organism: Escherichia coli
manufacture_protocol: 1. It contains 5,978 70mer oligos and a control set of 12 positive and 12 negative controls.
  2. A total of 19086 spots are printed at 200 um spacing in a 4 by 4 block of sub-arrays in triplicate in each slide.
  3. Oligos are dissolved to a final concentration of 18.75 uM in 150 mM NaPO4 buffer (pH 8.7) prior to printing.
  4. Slides are Corning Epoxide slides.
  5. Oligos represing 4,289 ORF's in Escherichia coli K12, 5,029 ORF's in O157:H7 (EDL933), and 4,836 ORF's in O157:H7 (Sakai).
  6. All probes are designed within ORFs from both clones and predicted ORF's.
  7. All probes are Tm nomalized to 75 (+/- 5) degree C and have a 5' amino linker.