title: Agilent-036891 Babesia bovis_UP array
status: Public on Apr 10 2015
contact.city: Pretoria
contact.first: Ingrid
contact.last: Rossouw
contact.email: s24075681@tuks.co.za
contact.state: Gauteng
contact.address: Lynnwoodroad
contact.department: Genetics
contact.country: South Africa
contact.institute: University of Pretoria
organism: Babesia bovis
manufacture_protocol: Custom B. bovis microarray slides were manufactured using a proprietary non-contact industrial inkjet printing process, in which oligo monomers were deposited uniformly onto specially-prepared glass slides. This in situ synthesis process printed 60-mer length oligonucleotide probes, base-by-base, from digital sequence files. The precise inkjet process enabled the delivery of extremely small, accurate volumes (picoliters) of the chemicals to be spotted. Standard phosphoramidite chemistry was used in the reactions which allowed for very high coupling efficiencies to be maintained at each step in the synthesis of the full-length oligonucleotide. The general cycle and process of oligo synthesis was repeated 60 times.
  Features were numbered Left-to-Right, Top-to-Bottom as scanned by an Agilent scanner (barcode on the left, DNA on the back surface, scanned through the glass), matching the FeatureNum output from Agilent's Feature Extraction software.