title: Exiqon miRCURY LNA ready-to-spot probe set  v.11.0 (miRBase v16.0)
status: Public on Nov 21 2013
contact.city: Leicester
contact.first: Dalbir
contact.middle: Kaur
contact.last: Dhiraj
contact.state: Leicestershire
contact.address: Lancaster Road
contact.department: MRC Toxicology Unit
contact.country: United Kingdom
contact.institute: Medical Research Council
organism: Mus musculus
  Rattus norvegicus
manufacture_protocol: miRCURY LNA probes (Exiqon, 208210-A v.11), generated from miRBase v16.0, were supplied dried down at 300 pmol concentration in Genetix 384 well plates. The full printing set consists of 6 plates. Prior to printing probes were resuspended in 3 x SSC, 1.5 M Betaine, 30% ethylene glycol to a final concentration of 20 µM. Plates were loaded onto an ArrayJet Ultra-Marathon II microarrayer; and the slides used were Genetix aldehyde plus. Printing was performed using an inkjet-style technology to deposit an equal quantity of probe on each slide, with 5 repeats down the slide. The layout of the print was 3 blocks, each one consisting of 32 columns and 32 rows. A marker plate was also included top and bottom to make aligning the features easier.