title: Agilent-025188 Custom Agilent Myzus Persicae Array
status: Public on Oct 07 2010
contact.city: Harpenden
contact.first: Chris
contact.last: Bass
contact.email: chris.bass@rothamsted.ac.uk
contact.state: Hertfordshire
contact.address: West Common
contact.department: Biological Chemistry
contact.country: United Kingdom
contact.institute: Rothamsted Research
contact.phone: 01582763133
organism: Myzus persicae
description: All sequences on which the array probes are designed are publically available at http://www.aphidbase.com/aphidbase/downloads under Myzus454(fasta) and MyzusSanger(fasta).
manufacture_protocol: The custom microarray used in this study was designed using the Agilent eArray platform (Agilent Technologies) by the Georg Jander Lab and is based on a previously described array containing probes for >10, 000 M. persicae unigenes produced by Sanger sequencing (Ramsey, Wilson et al. 2007) augmented with an additional 30,517 probe set designed on EST unigene sequences identified in a 454 sequencing project (Ramsey, Rider et al. 2010). The final slide layout consists of four arrays of 45,220 60-mer probes and these are produced by Agilent by in situ oligonucleotide synthesis. References: Ramsey, J. S., D. S. Rider, et al. (2010). "Comparative analysis of detoxification enzymes in Acyrthosiphon pisum and Myzus persicae." Insect Molecular Biology 19: 155-164. Ramsey, J. S., A. C. C. Wilson, et al. (2007). "Genomic resources for Myzus persicae: EST sequencing, SNP identification, and microarray design." BMC Genomics 8.