title: MRA-1001_miRHuman_16 ( LC science)
status: Public on Dec 10 2011
contact.city: Detroit
contact.first: Fazlul
contact.middle: H.
contact.last: Sarkar
contact.email: fsarkar@med.wayne.edu
contact.state: MI
contact.address: 4100 John R
contact.department: Pathology
contact.country: USA
contact.institute: Wayne State University School of Medicine
contact.phone: 313-576-8327
organism: Homo sapiens
manufacture_protocol: Arrays are made based µParaflo microfluidic technology (Atactic Technologies). On the microfluidic chip, each detection probe consisted of a chemically modified nucleotide coding segment complementary to target microRNA (from miRBase, http://microrna.sanger.ac.uk/sequences/) or other RNA (control or customer defined sequences) and a spacer segment of polyethylene glycol to extend the coding segment away from the substrate. The detection probes were made by in situ synthesis using PGR (photogenerated reagent) chemistry. The hybridization melting temperatures were balanced by chemical modifications of the detection probes.