title: CIMAP Papaver somniferum
status: Public on Dec 30 2013
contact.city: Lucknow
contact.first: Ashutosh
contact.middle: K
contact.last: Shukla
contact.email: ashupov@yahoo.com
contact.state: Uttar Pradesh
contact.address: P.O. CIMAP
contact.department: Biotechnology Division
contact.country: India
contact.institute: CSIR-Central Institute of Medicinal and Aromatic Plants
contact.phone: +91-522-2718553
organism: Papaver somniferum
manufacture_protocol: Five microgram of total RNA was used for amplification using Amino Allyl MessageAmp™ II aRNA Amplification Kit from Ambion by linear transcription based RNA amplification system to produce cRNA. Briefly, mRNA is reverse transcribed with a oligo (dT) primer bearing T7 promoter at 42°C for 2 hrs and second strand cDNA synthesis at 16°C for 2 hrs. The resulting cDNA was then purified and was then transcribed with T7 RNA polymerase to generate multiple copies of aminoallyl antisense RNA (aRNA) at 37°C for 16 hrs. aRNA is then labelled with Cy3TM/Cy5TM post-labelling reactive dye pack (GE Healthcare, UK) at room temperature and unincorporated Cy3/Cy5  molecules were removed by purification process.