title: NimbleGen custom Bacillus subtilis MAD-BS-2-Nimble-E-135
status: Public on Nov 28 2013
contact.city: Amsterdam
contact.first: Martijs
contact.last: Jonker
contact.email: m.j.jonker@uva.nl
contact.address: Science Park 904
contact.department: Microarray Department
contact.country: Netherlands
contact.institute: University of Amsterdam
organism: Bacillus subtilis
description: Design settings: Design based on NCBI Bacillus subtilis 168 uid57675 fasta sequence (26 jan 2011, Refseq:NC_000964.3, gi|255767013). Maximum of 135.000 probes. Design on both strands Probe length 60 bases Blast bitscore threshold of 80. Design results: 131.580 probes designed. Resulting tile-step of 32 bases. Overlap of 28 bases between probes (=gap-size -28) on opposite strands. Gap size of 4 bases between probes on the same strand. 303 Spike-in probes for 8 spike-in mRNA’s. 6.400 control probes (5000 “Alien” negative controls + 1400 Concentration Controls)
manufacture_protocol: Details on the manufacture protocol can be found on  http://www.nimblegen.com/technology/index.html