title: Multi-species Autoantigen Microarray 94
status: Public on Apr 18 2017
contact.city: Dallas
contact.first: yun
contact.last: lian
contact.email: yun.lian@utsouthwestern.edu
contact.state: TX
contact.address: 5323 Harry Hines Blvd.
contact.country: USA
contact.institute: UT Southwestern Medical Center
organism: Homo sapiens
  Mus musculus
  Rattus norvegicus
manufacture_protocol: Dilute peptides, proteins, protein complexes, or other antigens in PBS-0.1% Tween20 to 0.1 - 0.2 mg/mL.  Transfer diluted antigens transferred to 384-well print plates (15 μL/well). Antibody or other proteins/peptides prelabeled with Cy-3 and / or Cy-5 (diluted to 0.1 μg/μL) can be added to the print plate to serve as marker spots to orient the arrays post-scanning.  Using a biorobotic arrayer (MicroGrid 610) and quill pins (Genomic Solution) to print antigens on nitrocellulose coated microarray slides (FAST Slide, 16 pad, Whatman Inc). Slides were kept at 75% humidity chamber for 12 hours.  Store arrays at 4°in slide boxes sealed in plastic heat-sealable pouches. Reactivity is generally best within 1 month of printing, but printed antigens can retain reactivity for months.