title: Agilent-018561 Hunter C. elegans Oligo Microarray 15K (v1)
status: Public on Dec 31 2011
contact.city: Cambridge
contact.first: Daniel
contact.last: Schott
contact.state: MA
contact.address: 16 Divinity Avenue
contact.department: Molecular and Cellular Biology
contact.country: USA
contact.institute: Harvard University
contact.phone: 617 710 7091
organism: Caenorhabditis elegans
manufacture_protocol: Agilent SurePrint. We used a custom microarray (Agilent) bearing 60-mer oligonucleotide spots corresponding to 15,208 C. elegans genes.  Since this is far fewer than the number of recognized genes in C. elegans, we used an arbitrary procedure to select genes for inclusion on the array.  First, we limited the genes to predicted protein-coding genes, and then we prioritized genes with 1:1 best reciprocal sequence similarity scores to annotated C. briggsae genes (Remm et al., 2001).  We selected oligonucleotide sequences corresponding to the 3’-most predicted exon that allows design of a probe that minimizes cross-hybridization with other genes.  Gene positions on the array were randomized to minimize bias due to hybridization artifacts.