title: CIT-MMJGGL Arabidopsis Small RNA 2k v1.0
status: Public on Dec 01 2014
contact.city: Bellaterra
contact.first: Jose Luis
contact.last: Riechmann
contact.email: joseluis.riechmann@cragenomica.es
contact.state: Barcelona
contact.address: Campus UAB - Edifici CRAG
contact.country: Spain
contact.institute: Center for Research in Agricultural Genomics
organism: Arabidopsis thaliana
  Caenorhabditis elegans
  Drosophila melanogaster
description: Please note that the PRED* and MPSS* identifiers represent small RNA sequences from Arabidopsis that were either predicted (PRED) or identified through MPSS (massively parallel signature sequencing).
manufacture_protocol: 1. Oligos were synthesized with a 3' Amine C7 modification and arrayed in Genetix X7020 384-well plates. Each well contains 15 microliters of oligonucleotide at 50 micromolar in water.
  2.Plates are dried in speed vac and then resuspended in 18 microliters per well of 150 mM Phophate buffer, pH 8.5, for a final probe concentration of 40 micromolar in 150 mM of Phosphate buffer. Seal plates.
  3. Resuspend oligonucleotides overnight with shaking, near maximum rotational speed on flat-bed shaker.
  4. Centrifuge plates at 1000 rpm for 3 minutes.
  5. Remove seals and cover with plate lids. Place in appropriate location of plate holder.
  6. Print arrays on Code Link slides using a BioRobotics MicrogridII arrayer, Biorobotcs TAS software, and a 16-pin configuration.
  7. After print run is completed, block CodeLink slides using the standard CodeLink protocol, and pack slides under vacuum.
  8. After print run is completed, dry plates on speed vac. For each subsequent preparation of these plates for a print run, add water to the wells instead of sodium phosphate buffer.