title: Bacillus subtilis 168 spotted oligo array2
status: Public on Mar 27 2007
contact.city: Zeist
contact.first: Bart
contact.last: Keijser
contact.address: Utrechtseweg 48
contact.country: Netherlands
contact.institute: TNO
organism: Bacillus subtilis subsp. subtilis str. 168
description: GAPS II Corning
manufacture_protocol: Please note, the original microarray outline can be found in the attached file. This outline was to accomodate sample values based on the average vlaues of 4 technical replica's (library was spotted in duplicate and sample was hybridized in duplicate as well).
  The B.subtilus oligonucleotide libraries (Cat # BACLIB384) were obtained from Sigma-Compugen Incorporated. Technical support was supplied by LabOnWeb (http://www.labonweb.com/cgi-bin/chips/full_loader.cgi). The libraries represent in total 4128 oligos, which represent 4106 B subtilus genes, 10 controls from E.coli and the Brome mosaic virus and 12 random oligos LEADS™ clusters plus 231 controls. The oligonucelotide library was printed with a Lucidea Spotter (Amersham Pharmacia Biosciences, Piscataway, NJ, USA) on commercial UltraGAPS slides (amino-silane-coated slides, Corning 40017) and processed according to the manufacturer’s instructions.