title: NCI/ATC Hs-UniGEM2
status: Public on May 27 2004
contact.city: Gaithersburg
contact.first: David
contact.last: Petersen
contact.email: petersed@mail.nih.gov
contact.state: MD
contact.address: 8717 Grovemont Circle
contact.country: USA
contact.institute: NCI
contact.phone: 301-435-7898
organism: Homo sapiens
description: Printing by the NCI /ATC Microarray Facility
  Surface type: amino-silane coated glass Corning CMT-GAPS II (http://www.corning.com)
  Attachment: UV cross-linked
  Array description:  The custom arrays are manufactured by the NCI Advanced Technology Center Microarray Facility (NIH/NCI/CCR/ATC).  Microarrays are printed with GeneMachines robotic arrayer from Genomic Solutions (http://www.genomicsolutions.com) that is fitted with a printhead containing 32 SMP3 printing pins from Telechem International (http://www.arrayit.com).  The human cDNA probe set is based on 9128 PCR products from Incyte's human UniGEM 2.0 library (http://www.incyte.com).  The set includes 122 Incyte EST clones; 8998 identifed with a public GenBank Accession; 8905 Unique GenBank Accessions; 8882 mapped into Hs UniGene Build #131; 8281 Unique UniGene Clusters; 1179 EST clusters; 7102 "Named" clusters.The cDNA probes are distributed into 384-well polypropylene plates (Genetix, 384 micro array) and dissolved in 3XSSC print buffer at a concentration of 50-100 ug/ml.  The microarray spot densities are 235 microns (center-to-center), and one spot is printed per probe per slide.  The microarrays printed on Corning CMT-GAPS II slides (Item #40004) coated with amino-silane and post-processed according to the manufacturer's protocol.
manufacture_protocol: Microarrays are printed with GeneMachines robotic arrayer from Genomic Solutions (http://www.genomicsolutions.com) that is fitted with a printhead containing 32 SMP3 printing pins from Telechem International (http://www.arrayit.com). The human cDNA probe set is based on 9128 PCR products from Incyte's human UniGEM 2.0 library (http://www.incyte.com). The set includes 122 Incyte EST clones; 8998 identifed with a public GenBank Accession; 8905 Unique GenBank Accessions; 8882 mapped into Hs UniGene Build #131; 8281 Unique UniGene Clusters; 1179 EST clusters; 7102 Named clusters. The cDNA probes are distributed into 384-well polypropylene plates (Genetix, 384 micro array) and dissolved in 3XSSC print buffer at a concentration of 50-100 ug/ml. The microarray spot densities are 235 microns (center-to-center), and one spot is printed per probe per slide. The microarrays printed on Corning CMT-GAPS II slides (Item #40004) coated with amino-silane and post-processed according to the manufacturer's protocol.