title: UniversityofOregon Dmelanogaster CustomAgilent44K
status: Public on Apr 23 2009
contact.city: Eugene
contact.first: Michael
contact.last: Miller
contact.state: OR
contact.address: 1229 University of Oregon
contact.department: Institute of Molecular Biology
contact.country: USA
contact.institute: University of Oregon
organism: Drosophila melanogaster
manufacture_protocol: We used the Agilent (Palo Alto, CA) eArray platform to design a custom D. melanogaster oligonucleotide microarray representing 14,141 unique genes from the Flybase release 5.4 genome.  Briefly, we downloaded the dmel-all-transcript-r5.4.fasta file from Flybase which contains the sequences of every predicted transcript from genome release 5.4.  We used eArray to design two 60 bp oligo probes with a 3' bias for each transcript.  We removed duplicate probes and ordered microarrays with the remaining probe set.