title: UIUC/WMKCCFG Honeybee Sensory/CYP450 0.7K v1.0
status: Public on Aug 10 2007
contact.city: Urbana
contact.first: Hugh
contact.last: Robertson
contact.state: IL
contact.address: 505 S. Goodwin Ave.
contact.department: Entomology
contact.country: USA
contact.institute: University of Illinois at Urbana-Champaign
organism: Apis mellifera
description: Corning GAPS II
manufacture_protocol: Oligonucleotides (70-mer sense-strand) were designed using ArrayOligoSelector3.5 software (43, http://sourceforge.net) using default settings and the Apis mellifera genome (Assemby 2) for the following conceptual cDNA categories: 168 odorant receptors; 49 cytochrome p450s; 19 odorant binding proteins; 17 tetraspanins; 10 gustatory receptors; 10 carboxyl esterases; 7 glutathione S transferases; 6 chemosensory proteins (sensory appendage proteins); 5 lipocalins; 5 visual and non-visual opsins; 4 alpha-arrestins; 4 arrestins; 3 G-protein alpha subunits; 1 Pinocchio ortholog (a Drosophila melanogaster sensory-related protein); 1 sensory neuron membrane protein, and 4 housekeeping genes (cytoplasmic actin, EF1alpha, ribosomal protein 49, ribosomal protein S3), for a total of 313 genes. Oligonucleotides were synthesized by IDT (Coralville, IA) and 2.5 nmoles of each were aliquoted into a single 384-well plate and lyophilized.  Further preparation and microarray printing was performed by the Functional Genomics Unit at the U.I.U.C. W.M. Keck Center.  Oligonucleotides were suspended in 150 mM sodium phosphate to 25 uM and printed in neighboring duplicate spots on Corning GAPSII glass slides using a GeneMachines OmniGrid 100 microarray printer.  At least two housekeeping genes were spotted in each block of the 16-block array.  ArrayControl sense 70-mer oligonucleotides (Ambion) were diluted to 25 uM in printing buffer and printed as negative control spots. One control oligo of the 8 available was printed per block such that each control oligo was present in two different blocks per microarray.