title: [Agilent-068823] Agilent USC-Acuigen Perkinsus olseni 15 K v 1.0 ID-068823
status: Public on Dec 27 2019
contact.city: LUGO
contact.first: Belen
contact.middle: G
contact.last: Pardo
contact.email: belen.gomez@usc.es
contact.state: LUGO
contact.address: Avda. Carballo Calero s/n
contact.department: Zoology, Genetics and Physical Anthropology
contact.country: Spain
contact.institute: University of Santiago de Compostela
contact.phone: +34 982822428
organism: Perkinsus olseni
manufacture_protocol: We selected 10,104 sequences from our previously published P. olseni trophozoite transcriptome (Hasanuzzaman et al., 2016). A total of 9,369 sequences were selected because they were annotated to transcripts in the NCBI nr protein database, while the remaining 735 non-annotated sequences were selected by their notable differential expression in our preliminary evaluation (Hasanuzzaman et al., 2016). One oligo-probe was designed for annotated sequences (known sense) and two probes (sense and antisense) were designed for the non-annotated sequences. We also included 4,158 technical replicates for microarray reproducibility evaluation. The probes were synthesized in a random layout using the Agilent methodology.