title: Pinus taeda cDNA
status: Public on Jul 31 2006
contact.city: Raleigh
contact.first: Chris
contact.last: Smith
contact.state: NC
contact.address: 840 Main Campus Dr
contact.department: Bioinformatics Research Center
contact.country: USA
contact.institute: NCSU
organism: Pinus taeda
description: The microarray contains ESTs selected from 6 Xylem libraries: 1. NXCI - ESTs from juvenile compression wood of Pinus taeda (loblolly pine) 2. NXLV - ESTs from transitional/mature late wood of Pinus taeda (loblolly pine) 3. NXNV - ESTs from mature normal wood of Pinus taeda (loblolly pine) 4. NXPV - ESTs from transitional planings wood of Pinus taeda (loblolly pine) 5. NXRV - ESTs from root wood of Pinus taeda (loblolly pine) 6. NXSI - ESTs from juvenile side wood of Pinus taeda (loblolly pine) , a Pollen Cone library, and a Shoot tip library.
manufacture_protocol: DNA from 2109 ESTs was printed in two replications onto amino-silane coated CMT-GAPS slides (Corning Inc; Corning, NY, USA) using a Lucidea Array Spotter (Amersham Biosciences, USA). After printing, the DNA was cross linked using 250 mJ of UV-C radiation. The slides were subsequently baked at 75ºC for 2 hrs.