title: NUIG_salmon_5K array_V2.0 (full array layout)
status: Public on Dec 31 2009
contact.city: Galway
contact.first: Michael
contact.last: Cairns
contact.email: michael.cairns@nuigalway.ie
contact.address: University Road
contact.department: NCBES
contact.country: Ireland
contact.institute: NUI Galway
contact.phone: 0035391492094
organism: Oncorhynchus mykiss
  Salmo salar
description: Platform data table reflects full array layout in which all duplicate array features are represented.
  For funding reasons only those spots identified in subsequent microarray analysis as interesting were sequenced
  Every sequenced spot has a Genbank Accession Number associated with it.
manufacture_protocol: The cDNA microarray contained clones from a number of Suppression Subtractive Hybridisation (SSH) cDNA libraries constructed from different salmon tissues (brain, pituitary, hypothalamus, head kidney, spleen, intestine, gill, testes and ovary) at different developmental stages related to sexual maturation, smoltification and embryo development. Of the 5376 cDNA clones, 4128 were newly generated subtracted cDNA clones and the remaining 1248 clones were other SSH or full-length clones from related salmonid projects. Several candidate genes and controls were also included. At the printing stage DNA sequences were only known for those clones that were used to check library quality/redundancy and for those clones that originated from related salmonid projects. Otherwise clones were only sequenced after microarray analysis had identified a differentially expressed gene. Printed slides consisted of duplicate arrays with sub-arrays in a 4 x 4 format with 336 spots per sub-array.