title: Agilent-023387 Rhagoletis pomonella custom cDNA array
status: Public on Oct 01 2011
contact.city: Gainesville
contact.first: Greg
contact.last: Ragland
contact.email: gragland@ufl.edu
contact.state: FL
contact.address: PO Box 110620
contact.department: Entomology and Nematology
contact.country: USA
contact.institute: University of Florida
organism: Rhagoletis pomonella
description: Probesets:
  CUST_1_PI414333030 to CUST_23040_PI414333030: two forward probes for each annotated R. pomonella EST.
  CUST_1_PI414333074 to CUST_11521_PI414333074: one reverse (minus strand) probe for each annotated R. pomonella EST.
  CUST_1_PI414333083 to CUST_3770_PI414333083: two forward probes for each unannotated R. pomonella EST.
  CUST_1_PI414333086 to CUST_1886_PI414333086: one reverese probe for each unannotated R. pomonella EST.
  CUST_1_PI415094689 to CUST_20_PI415094689: forward probe for each of 20 R. pomonella house keeping genes (for QC, replicated 10 times each on array).
  CUST_1_PI414333099 to CUST_356_PI414333099: two forward probes from diapaue candidate genes in Drosophila melanogaster (note: poor hybridization).
  CUST_1_PI414333102 to CUST_178_PI414333102: reverse probes from diapause candidate genes in Drosophila melanogaster.
  CUST_1_PI414333089 to CUST_2340_PI414333089: two forward probes from diapaue candidate genes in Sarcophaga crassipalpis (note: poor hybridization).
  CUST_1_PI414333095 to CUST_1171_PI414333095: reverse probes from diapause candidate genes in Sarcophaga crassipalpis.
  NOTE: all reverse probes for annotated genes included to establish distribution-based presence/absence calls if desired.
manufacture_protocol: 60-mer oligo probes designed from R. pomonella EST contigs and singletons using Agilent eArray v2.0 and synthesized in situ by an ink-jet printing (Agilent) method on glass slides.