title: NSF_Pinus_taeda_49K
status: Public on May 23 2007
contact.city: Rockville
contact.first: Jia
contact.last: Liu
contact.email: jliu@jcvi.org
contact.state: MD
contact.address: 9712 Medical Center Drive
contact.country: USA
contact.web_link: http://www.tigr.org/tdb/potato
contact.institute: Plant Genomics
organism: Pinus taeda
description: The below table is based on the GAL file. This platform may not be suitable for submission of data in which replicate features have been averaged during normalization. In such cases users should submit their own platform representing merged features.
manufacture_protocol: The elements on the microarray were selected from the pine embryo-derived cDNA libraries sequenced at TIGR in collaboration with Dr. John Cairney as part of the NSF Genomics of Loblolly Pine Embryogenesis Project. Details on the sequencing and the origins of the cDNA libraries of these clones can be found at: http://www.tigr.org/tdb/e2k1/pine/pine_status.shtml.
  A total of 16,992 cDNAs were selected for the microarray. The pine cDNA clones were rearrayed, resequenced, and the inserts were amplified using universal primers that flank the insertion site. All PCR products were run on agarose gels to check for amplification of single bands. Only 12,536 clones could be verified by both resequencing and agarose gel inspection. However, all 16,992 cDNAs were spotted in duplicate on the slides.
  Methods used successfully at TIGR with pine microarrays can be found at http://www.tigr.org/tdb/e2k1/pine/pine_protocols.shtml.