title: URGV Arabidopsis thaliana 25K CATMA_v2
status: Public on Jun 01 2007
contact.city: Evry
contact.first: Frederique
contact.last: Bitton
contact.email: bitton@evry.inra.fr
contact.address: 2 rue Gaston Cremieux
contact.country: France
contact.web_link: http://www.evry.inra.fr
contact.institute: INRA
organism: Arabidopsis thaliana
description: Corning Ultra-GAPS (TM) slides are spotted with 64 pins (8x8) therefore the spotted area is divided into 3 metablocks, each containning 64 blocks of 144 spots (12x12). The array contains 24576 Gene Specific Tags (GST)
manufacture_protocol: A.thaliana genes were predicted with Eugene software (Foissac S. et al., Nucleic Acids Res., 2003) and gene-specific sequence tags were automatically designed (Thareau et al. Bioinformatics, 2003 Nov 22). Two rounds of PCR were performed and PCR products were finally purified and dissolved in 50% DMSO in TE at concentrations ranging between 50-200ng/ul and re-arrayed in 1536 microtiter plates. The pins are washed in 0.1% SDS at 50 °C then three times in bi-distilled water. Spotter settings are 45-55% humidity, and a pitch = 180 um. The room temperature is set between 19 and 21 degrees Celsius and below 40 % humidity. The microtiter plates are placed in the biobank. The four trays are cleaned and 100-120 slides placed on them (30 slides per tray). The spotting lasts 25 hours. The slides are stored in the dark in a dry-cabinet for 3 days and UV-cross-linked at 300 mJ. After crosslinking, the slides are stored in the dark in a dry-cabinet.
  24576 Gene Specific Tags (GST)