title: University of Guelph, 1152 CfMNPV genes array version 1
status: Public on Jan 08 2010
contact.city: Guelph
contact.first: Dan-Hui
contact.last: Yang
contact.email: dyang@uoguelph.ca
contact.state: Ontario
contact.address: 488 Gordon Street
contact.department: Dept. of Molecular and Cellular Biology
contact.country: Canada
contact.web_link: http://www.uoguelph.ca/mcb
contact.institute: University of Guelph
contact.phone: 1-519-8244120 Ext.53264
organism: Choristoneura fumiferana multiple nucleopolyhedrovirus
description: The array included 292 oligonucleotide probes representing 146 CfMNPV ORFs, one for the coding strand and the other for the non-coding strand (for detection of antisense transcription). Purified CfMNPV viral genomic DNA in 10-fold serial dilutions was used as a positive control. A mixture of 20 viral oligonucleotides was served as a second positive control. The array included 2 oligos (At1 and At2) of Arabidopsis chlorophyll synthase for normalization between arrays, and 2 oligos (HAT4, YaaJ) as negative controls. The array also included 5 host insect internal control oligos. Each probe was printed in triplicate.
  
  This array represents a condensed array. The full array layout linked as a supplementary file.
manufacture_protocol: Spotted 50-mer nucleotides on glass slides