title: Streptococcus pyogenes (Group A Strep) amplicon array
status: Public on Oct 03 2011
contact.city: Baltimore
contact.first: NIKHIL
contact.last: KUMAR
contact.email: nkumar@som.umaryland.edu
contact.state: MD
contact.address: 801 W. Baltimore Street
contact.department: Institute for Genome Sciences
contact.country: USA
contact.institute: University of Maryland, Baltimore
organism: Streptococcus sp. 'group A'
manufacture_protocol: Pan-genome microarray was constructed using Alab49 as the reference.
  PCR primers were designed for predicted genes in Alab49 using Primer3, followed by 11 subsequent strains not already represented by Alab49 and were tested for uniqueness using in-house scripts.
  The resulting 3388 amplicons ranged in size from 51 to 1170 bp; all of these were amplified using 10X PCR Buffer, MgCl2, 100mM dNTP mix, Taq Polymerase, and water (all Applied Biosystems).
  30uL of amplicon are in 384-well Genetix plates in 50% DMSO, which is used as printing buffer.
  Amplicons are spotted in triplicate on Corning Ultra-GAPS aminosilane slides, using a Lucidea (GE Healthcare) or Aushon Biosystems (www.aushon.com) microarray-printing robot.
  After print run, slides cross-linked at 750E.