title: E and NS1 proteins of dengue and Zika viruses GA_DZ_V2
status: Public on Oct 11 2023
contact.city: San Lorenzo
contact.first: Victor
contact.middle: Hugo
contact.last: Aquino
contact.email: vhaquino@iics.una.py
contact.state: Central
contact.address: Dr. Cecilio Báez c / Dr. Gaspar Villamayor
contact.department: Research Institute for Health Sciences
contact.country: Paraguay
contact.institute: National University of Asuncion
organism: Dengue virus
  Zika virus
manufacture_protocol: Amino acid microparticles are laser printed onto a glass slide with a custom 24-color laser printer. One layer of an amino acid particle pattern is printed on a coated glass slide. A heating step melts the particles. Embedded amino acids can couple to the chip surface. In accordance with solid phase Fmoc chemistry, a cycle of synthesis is completed after removal of excessive material by washing and cleavage of the N-terminal Fmoc protection group. The cycle is repeated until the full peptide microarray is synthesized.