title: PDP Mouse 11k BMAP
status: Public on Jan 25 2008
contact.city: Winnipeg
contact.first: stephanie
contact.last: booth
contact.email: Stephanie_Booth@phac-aspc.gc.ca
contact.address: 1015 Arlington St
contact.country: Canada
contact.institute: PHAC
organism: Mus musculus
manufacture_protocol: Libraries and clones derived from brain regions of adult mouse (strain C57BL/6) were obtained from Research Genetics. The 11 136 clones represent expressed sequence tags (ESTs) from some 5471 unique known genes and 5665 as-yet-uncharacterized mRNAs and form part of a library that was created as part of the Brain Molecular Anatomy Project (BMAP). Each insert from this BMAP 3' EST library was amplified by PCR. cDNA was purified by using Millipore multiwell purification plates and lyophilized PCR products were resuspended in 1x Micro-spotting Solution Plus (TeleChem) at a concentration of 0·25–0·75 µg µl–1. DNA was spotted onto CMT–GAPS-coated glass slides (Corning) by using Stealth micro-spotting pins (TeleChem)