title: UNESP Xylella fastidiosa array version 1
status: Public on Nov 27 2008
contact.city: Berlin
contact.first: Tiago
contact.last: Lopes
contact.email: tiagojab@yahoo.com.br
contact.address: Robert Rossle Strasse, 10
contact.country: Germany
contact.institute: Max-Delbruck Center for Molecular Medicine
organism: Xylella fastidiosa
manufacture_protocol: The amplified products were suspended in 50% v/v DMSO in a final concentration of 100 to 300 ng/microliter and arranged in duplicate at a distance of 250 micrometer in glass slides treated with aminosilane (Corning). The printing of the microarrays was done by a robot model GMS 417 Arrayer (Affymetrix Inc.). After printing, the DNAs were re-hydrated (42 degrees C for 10 s), dried (70 degrees C for 1 min) and fixed in a UV camera cross-link (1300 x 100 microJ cm2). The slides were kept at 70 degrees C for 2 h and then stored at room temperature under vacuum. Genetically distant negative controls were also included in this array, consisting of human genes (pHUM1 and pHUM7) and plant genes (707050B11 - Rubisco), as well as synthetic controls from various species (human, mouse, Arabidopsis spp., Archaeabacteria and E. coli) obtained by the Lucidea Universal ScoreCard kit (Amersham Biosciences).