title: Grossman Lab BSS1-3
status: Public on Oct 16 2009
contact.city: Cambridge
contact.first: Alan
contact.middle: D.
contact.last: Grossman
contact.email: clee2@mit.edu, adg@mit.edu
contact.state: MA
contact.address: 31 Ames Street, 68-530
contact.department: Biology
contact.country: USA
contact.institute: MIT
contact.phone: 617-253-6702
organism: Bacillus subtilis
manufacture_protocol: Oligonucleotide library of B. subtilis from Sigma-Genosys (catalog number BACLIB96, consisting of 4106 annotated genes in the Subtilist genome data release R16.1) and five additional custom oligonucleotides designed by Grossman lab spotted on Corning GAPS II slides using Genetix Q-Array Mini Printer. The sequences for the five Grossman lab custom probes are as follows:
  
  dnaN2
  GGAGTATCCTTTACAGGGAGTGACTCAGATATTTCTATTGAATCCTTCATTCCAAAAGAAGAAGG
  
  dnaN3
  CAGTATTTGACGATTATCCGTTCTGGTAAAGCTGAATTTAATCTAAACGGACTGGATGCTGATGA
  
  pep1
  ATGAAAAAGGCTTATAAGAAACCTCGTCTTTTTGAACTGCGCCGTGCTGTAGCAGCTGGCGGCCAT
  
  pep2
  ATGAAAAAAGCTTATAAGAAACCTCGCCTTTTTGAACTGCGCCGTGCTGTAGCAGCTGGCGGCCAT
  
  pep3
  ATGAAAAAAGCTTATAAGAAACCTCGCCTTTTTGAATTGCGCCGCGCTGTAGCAGCTGGCGGTCAT
  