title: LBEG Prunus persica 4.8K
status: Public on Oct 13 2011
contact.city: Santiago
contact.first: Leonardo
contact.middle: Ignacio
contact.last: Pavez
contact.state: Santiago
contact.address: Av Manuel Montt #948
contact.country: Chile
contact.institute: Universidad de Las Américas
organism: Prunus persica
manufacture_protocol: 1.Oligos are arrayed in Genetix 384 Well Microarray Plates (Cat# X7020). Each well contains 600 pmol of 70-mer oligo.
  2. Resuspend oligos in 17 µL of Telechem Microspotting solution 1X.
  3. Seal plates with Corning seals and after oligos resuspenction print probe with the MicroGrid II Biorobotics spotter.
  4. Print is performed with 48 Stealth pins.
  5. After printing run plates are stored at -20 C degree.
  6. Every 4 months plates are lyophilized and resuspended in adjusted water volume according to the number of spotting runs. This, to maintain the same volume and concentration in all plate well.