title: ISU_Minion_Mhyo_IG-oligo_v1.0
status: Public on Jun 30 2010
contact.city: Ames
contact.first: Chris
contact.last: Minion
contact.email: fcminion@iastate.edu
contact.state: IA
contact.address: 1130 Vet Med
contact.department: VMPM
contact.country: USA
contact.institute: Iowa State University
contact.phone: 515-294-6347
organism: Mesomycoplasma hyopneumoniae
manufacture_protocol: Probes were designed for the intergenic regions of Mycoplasma hyopneumoniae. Intergenic regions were defined as noncoding DNA sequences between annotated genes in the M. hyopneumoniae genome sequence (GenBank accession # AE017332). Intergenic regions larger than 50 bp were considered for analysis, and those between 125-450 bp were considered for PCR-based probes. Oligonucleotides were designed for some intergenic regions based on the inability to produce PCR products or the length of the region (50-125 bp). Oligonucleotides ranged in size from 50 to 60-mers and were synthesized by Integrated DNA Technologies, Inc. (IDT, Coralville, IA). Oligonucleotides were resuspended to 100 pmol/μl in nuclease free water and then 9 μl were vacuum dried and diluted to 30 μM in IDT Epoxide spotting buffer. These oligonucleotides were spotted to Corning Epoxide substrates in triplicate.