title: NIAID Mouse 17K v1.0
status: Public on Oct 01 2021
contact.city: Ankara
contact.first: Ihsan
contact.last: Gursel
contact.email: ihsangursel@bilkent.edu.tr
contact.state: Cankaya
contact.address: Faculty of Science, Ihsan Dogramaci Bilkent University
contact.department: Molecular Biology and Genetics
contact.country: Turkey
contact.institute: Bilkent University
contact.phone: +90-312-290-2408
organism: Mus musculus
manufacture_protocol: The mouse genome oligonucleotide probe set contains 16,896 optimized 70mers (plus controls) obtained from Operon (http://www.operon.com/).
  The oligonucleotide probes were purchased in 384-well polypropylene plates (Genetix, 384 micro array) and dissolved in 4XSSC print buffer at a concentration of 20-30 uM.
  The microarray is composed of 32 blocks and one spot is printed per probe per slide.
  The spot size is 100 micron and spot distance is 185 microns (center-to-center).
  The microarrays were printed on Gold Seal micro slides (Cat #3010) coated with poly-L-lysine and post-processed according to Operon's protocol.
  Overall, polylysine coating on glass support constitutes the main backbone of the microarrays.