title: Bifidobacterium breve 210B array
status: Public on Mar 09 2010
contact.city: Verona
contact.first: Alberto
contact.last: Ferrarini
contact.email: alberto.ferrarini@univr.it
contact.state: Veneto
contact.address: Strada le Grazie, 15
contact.department: Scientific and Technological Department
contact.country: Italy
contact.institute: University of Verona
contact.phone: +39-045-802-7058
organism: Bifidobacterium breve
description: A total of 2110 probes, representing all the identified ORFs of B. breve 210B genome, of 35-40 bp in length were designed using OligoArray 2.1 software. Oligos were synthesized in 18 replicates on a 2x40k CombiMatrix array (CombiMatrix, Mulkiteo, USA). Replicates were distributed on the chip at random, non-adjacent positions. A set of 29 negative control probes designed on phage and plant sequences were also included on the chip in 60 replicates at randomly distributed positions.
  Spots size: 25 microns
  Total number of spots: 94928
  The Platform data table reflects a condensed representation of the array's replicate features.  The full array layout (gal file) representing all of the individual features is linked as a supplementary file at the foot of this record.
manufacture_protocol: Probes are directly synthesized on a 90k Combimatrix array by an in situ oligo synthesis system developed by Combimatrix Corporation (Mukilteo, USA) using the Combimatrix CustomArray synthesizer. Standard synthesis protocol provided by Combimatrix was used.