title: National Engineering Research Center mammalian microRNA microarray
status: Public on Feb 11 2010
contact.city: beijing
contact.first: wu
contact.last: jp
contact.email: jpwu@capitalbio.com
contact.address: 18 Life Science Parkway
contact.country: China
contact.institute: National Engineering Research Center
organism: Homo sapiens
  Mus musculus
  Rattus norvegicus
description: Altogether 924 mature miRNA sequences were assembled and integrated into our miRNA microarray design, these comprised 677 human (including a further 122 predicted miRNA sequences from published references (Xie et al., Nature 2005,434:338-345 ), 292 rat and 461 mouse mature miRNAs from the miRNA Registry (Sanger, accessed Aug. 2007). In addition, we designed 8 short oligonucleotides that possessed no homology to any known RNA sequence and we produced their corresponding synthetic miRNAs by in vitro transcription using Ambion's miRNA Probe Construction kit (Cat. No.1550, Austin, TX). We added various amounts of these synthetic miRNAs into the human miRNA samples prior to analysis as internal controls.
manufacture_protocol: as described in CapitalBio lab manual