title: Institut Pasteur de Lille Human MMP & ADAM long oligo array
status: Public on May 12 2010
contact.city: Lille
contact.first: David
contact.last: Hot
contact.email: david.hot@pasteur-lille.fr
contact.address: 1 rue du professeur Calmette
contact.department: Center for Infection and Immunity of Lille
contact.country: France
contact.institute: Institut Pasteur de Lille
organism: Homo sapiens
description: Spotted long oligonucleotides.
manufacture_protocol: 1. Oligo were purchased at MWG-biotech In 96-well plates (Abgene 1058). Each oligo was resustended in QMT2 buffer (Corning) at 50 µM final
  2. Oligo solutions were spotted using Affymetrix 427 pin-and-ring arrayer on aldehyde fonctionnnalised slides (Genetix) at 22°C and 60-70% relative humidity.
  3. Glass slides were hydrated in saturated humidity chamber at 42°C overnight. Glass slides were then treated in 132mM NaBH4 in PBS containing 25% ethanol at 42°C during 8h.
  4. Glass slides were washed once in 0.2% SDS at 42°C during 2 minutes and twice in distilled water and finally dried by centrifugation.
  5. Slides were pre-hybridized with 15µl of hybridization buffer at 42°C during 1h just before hybridization.