title: Nanostring nCounter PanCancer Immune Profiling Panel
status: Public on Oct 01 2024
contact.city: Parkville
contact.first: Alesha
contact.middle: A
contact.last: Thai
contact.email: alesha.thai@petermac.org
contact.state: VIC
contact.address: Department of Medical Oncology, 305 Grattan St
contact.country: Australia
contact.institute: Peter MacCallum Cancer Centre
organism: Homo sapiens
manufacture_protocol: Three specifically-designed unique oligonucleotides constitute the heart of each nCounter probe. Based on the target gene sequence, two 50mer DNA oligos are designed to complement opposite ends of a unique 100 bp stretch of the gene. Attached immediately adjacent to one 50mer oligo is an additional DNA oligo sequence, known as the backbone, that anneals to a complementary RNA molecular barcode. Individual segments of the RNA oligo are labeled with specific fluorophores arranged in various specific linear orders to create a unique code for each gene of interest (Geiss). This DNA-RNA hybrid molecule constitutes the reporter half of the probe pair. The second half of the two-part probe consists of the second gene-specific sequence taken from the adjacent end of the 100 bp target sequence, forming the capture probe (Geiss).    Hybridization of the reporter and capture probes to the target RNA creates a tripartite structure identified by the specific fluorescent signal of the reporter probe, which can also be used for quantifying each transcript.