title: Bovine Long Oligo Plus Array (BLOPlus) 10K
status: Public on Sep 15 2009
contact.city: Guelph
contact.first: Chris
contact.middle: P
contact.last: Verschoor
contact.email: cverscho@uoguelph.ca
contact.state: ON
contact.address: 50 Stone rd. East
contact.department: APS
contact.country: Canada
contact.institute: University of Guelph
organism: Bos taurus
manufacture_protocol: Original BLO:
  The CAFG’s mission to improve specificity, slide reproducibility, spot
  morphology, background and ease-ofuse led to the work in 2004 on a bovine long oligo (BLO) microarray. This set of 70-mer oligos was derived from 13,914 ESTs in the NBFGC library. Using the Basic Local Alignment Search Tool (BLAST), sequences from NBFGC were compared to the Reference Sequence (RefSeq-2004) database at the National Center for Biotechnology Information (NCBI). This search yielded 7,449 unique
  genes. Oligo design was performed by bioinformaticians at the CAFG and the oligos were manufactured by Operon Biotechnologies, Inc. BLO slides were first printed in early 2005.
  BLO + extension set = BLOPlus:
  The BLOPlus microarray is a second generation bovine long oligo set.
  BLOPlus contains all oligos from the original BLO microarray plus an
  additional 1,865 bovine sequences. These sequences were selected from the highly published BOTL cDNA microarray library. BOTL originated from a
  normalized EST library derived from bovine leukocytes. The original
  microarray contained 720 unique, sequenced genes. Over the years other
  genes were added to the BOTL microarray, including a set of PCRgenerated sequences, eventually totaling 1,391.
  The aging BOTL cDNA resource needed to be updated and replaced.
  Producing an extension set of 70-mer oligos filled this need. The extension set also added genes important for studying bovine immunobiology to our bovine oligo microarray. A search in The Institute
  for Genomic Research (TIGR) database for the 1,391 BOTL sequences
  yielded a match to 1,330 of these sequences. Fifteen Kyoto  encyclopedia of Genes and Genomes (KEGG) pathways were also investigated yielding another 550 sequences. Many of the genes selected had TIGR tentative consensus (TC) 70-mer sequences. These were used for the oligos where available. A total of 305 70-mer sequences were generated by the CAFG using OligoPicker1 software.
  New oligo sequences were manufactured by Operon Biotechnologies, Inc.
  in January 2008. The sequences for all of the 70-mer oligos that appear on BLOPlus (Box taurus (bovine) AROS V1.1) are available for download at www.operon.com in the Oligo Microarray Database (OMAD).
  The BLOPlus microarray is printed at the Research Technology Support
  Facility (www.rtsf.msu) at Michigan State University. The substrate is the SuperAmine slide from ArrayIt Microarray Technologies
  (www.arrayit.com). This substrate contains positively charged, linear
  primary amines attached covalently to the glass surface allowing for
  attachment of the negatively charged DNA backbone.