title: Ion Channel specific Human Oligo Array
status: Public on Jun 17 2010
contact.city: Hamburg
contact.first: Christoph
contact.last: Ogrodowczyk
contact.address: Falkenried 94
contact.department: Institute for Neural Signal Transduction
contact.country: Germany
contact.institute: ZMNH
organism: Homo sapiens
manufacture_protocol: 1.Oligos are arrayed in Greiner 384-well flat-bottom plates, resuspended in betaine spotting-buffer (3x SSC, 1.5M Betaine). Vortex vigourously
  2. Place plate into spotting roboter (Tecan). Each Oligo was printed onto a lysine-coated glas slide in replication (4x).
  3. After printing slides were placed in vacuum for 2h. Drying was followed by crosslinking with a total energy of 60mJ.
  4. Printing process was controlled by sybr-green staining (s. Battaglia, 2000 Analysis of DANN Microarrays by non-destructive fluorescent staining using sybr-green II).