title: Oligoarray Muenster-Hematology, Version 2
status: Public on Sep 16 2008
contact.city: Muenster
contact.first: Carsten
contact.last: Müller-Tidow
contact.email: muellerc@uni-muenster.de
contact.address: Domagkstrasse 3
contact.country: Germany
contact.institute: University Hopital Muenster
organism: Homo sapiens
description: - Total of 33696 spots
  - Each of the 11000 genes is covered by ~3 different oligos (differing in their distance from the transcriptional start site (TSS), i.e., between ~1000 upstream and ~+500 downstream of the TSS)
manufacture_protocol: Oligonucleotides (25 µM) in 1.5 M betaine, 3xSSC (1xSSC is 0.15 M sodium chloride, 0.015 M sodium citrate) were printed onto Nexterion Slide E (Schott AG, Mainz, Germany) using the MicroGrid II 610 spotter (BioRobotics, Cambridge, UK) equipped with 48 SMP3 stealth pins (TeleChem International, Sunnyvale, CA, USA) delivering ~600 pl per spot. The spotting procedure was performed at 22°C, rel. humidity 40%. DNA was cross-linked to the surface by incubation of the arrays for 2h at 85 °C. (Oligonucleotides were purchased from Operon Biotechnologies GmbH, Nattermannallee 1, 50829 Koeln, Germany)