title: FSU-Schco1-Microarray v1.0
status: Public on Jun 15 2012
contact.city: Jena
contact.first: Erika
contact.last: Kothe
contact.email: erika.kothe@uni-jena.de
contact.address: Neugasse 25
contact.department: Microbial Communication, Institute of Microbiology
contact.country: Germany
contact.institute: Friedrich-Schiller-University
organism: Schizophyllum commune
description: The microarray used in the present study contains probes for the 13,181 predicted gene models. It is based primarily upon the FilteredModels3 of Schizophyllum commune v1.0 genome project. Corresponds to S. commune strain H4-8 (A4,6; B3,2).
manufacture_protocol: The probe synthesis, sample labeling, hybridization and detection was performed by manufacturer. The oligonucleotides (probes, 50-mers) were synthesized in situ, using standard DNA synthesis reagents and 3`-phosphoramidites carrying a 5`-photolabile protective group, and spotted on a glass surface. The MassageAmpTM-Biotin Enhanced RNA Kit from Ambion was used for the labeling of RNA. Hybridization was done automatically over night (16 hours) at 45°C using RT-Analyzer from febit. The biotin labeling was detected with streptavidinphycoerythrin (SAPE), in combination with Consecutive Signal Enhancement (CSE) procedure from febit. The feature recognition, using Cy3 filter set, and signal calculation were done automatically within milliseconds.