title: Trichophyton rubrum cDNA microarray 1
status: Public on May 04 2010
contact.city: Beijing
contact.first: Qi
contact.last: Jin
contact.email: zdsys@vip.sina.com
contact.state: Beijing
contact.address: No.6 Rongjing East Street, BDA
contact.country: China
contact.institute: National Institute for Viral Disease Control and Prevention
contact.phone: 8610-67876915
organism: Trichophyton rubrum
description: The PCR product were  spotted  onto  CMT-GAPSII-coated  slides
  (Corning  Glass).
manufacture_protocol: PCR  fragments used  for  printing  the microarray  chip were amplified  from  the EST library  in  96-well  plates  using  vector-PCR  amplification  with  T7  and  SP6  universal primers  on  the GeneAmp  PCR  system  9600  (Perkin Elmer,  Foster City, CA).  PCRs were initiated with DNA denaturation (5 min at 96 ºC) followed by annealing for 30
  s at 55 ºC and extension  (1 min and 30 s at 72 ºC)  steps carried out  for 35 cycles. PCR products were puried and dried  and  then  resuspended  with  a concentration of 100 ng/ μL.