title: Whole Genome Equine Array
status: Public on Mar 31 2013
contact.city: College Station
contact.first: Priyanka
contact.last: Kachroo
contact.email: pkachroo@cvm.tamu.edu
contact.address: University Drive
contact.country: USA
contact.institute: Texas A&M University
organism: Equus caballus
manufacture_protocol: 1. 15ul of 3X saline-sodium citrate (SSC) buffer to each well of the 300pM oligonucleotide set to make a 20uM oligonucleotide solution
  2. Transfer to an orbital shaker for 1 hour at room temperature with speed of 100rpm. At the end of 1 hour, oligos are ready to be printed
  3. The Texas A&M equine oligoarrays were printed on GAPS coated glass (Corning) with  aminosinale surface chemistry using using Telechem Type SMP3 printing pins
  4. Printing of the array was done by maintaining 30% realtive humidity and by using 48-pin in the print-head, redipping pins after every 50 slides
  5. Slides were then maintained at 80 degree temperature for 2 hours to complete the printing process. The slides were sealed and stored at room temperature in absence of light and moisture.