title: A-UMCU-M35K-1.0
status: Public on Oct 14 2011
contact.city: Utrecht
contact.first: Marian
contact.last: Groot Koerkamp
contact.state: Utrecht
contact.address: Heidelberglaan 25
contact.department: Research
contact.country: Netherlands
contact.institute: Princess Maxima Center for Pediatric Oncology
organism: Mus musculus
manufacture_protocol: 1. 70-mers from 384-well plates are printed with a BioRobotics MicroGridII spotter in an environment controlled room
  2. Humidity is 50% ± 3%
  3. Temperature is 18C in the spotter
  4. Quill Pins type MicroSpot2500 (BioRobotics) are used for spotting
  5. The printed slides are treated with UV-light after spotting by using a Stratalinker2400 from Stratagene: Energy 2400 mJ, Time 10 minutes (settings 23744 x 100 uJoule)
  6. The Stratalinker works with 254 nm UV-light and irradiates the slides with circa 4000 uWatt/cm2
  7. After UV-crosslinking, slides are stored in a desiccator-cabinet