title: Operon human v3 ~35K 70-mer two-color oligonucleotide microarrays.
status: Public on Dec 11 2008
contact.city: Groningen
contact.first: Rudolf
contact.middle: Stephan Nicolaas
contact.last: Fehrmann
contact.email: r.s.n.fehrmann@og.umcg.nl
contact.state: Groningen
contact.address: P.O. Box 30.001
contact.department: Gynecologic Oncology
contact.country: Netherlands
contact.institute: University Medical Center Groningen
organism: Homo sapiens
manufacture_protocol: Operon v3.0 oligo arrays from the central microarray facility (CMF) at the Netherlands Cancer Institute (NKI) were used for hybridization. A complete list of genes and controls present on the slides is available on the CMF web site (http://microarrays.nki.nl/download/geneid.html). The 70-mer oligo’s, dissolved in a 3x SSC solution, were printed on UltraGaps slides (Corning, Cat# 40018) with a BioRobotics MicroGrid II (Genomic Solutions) print robot. The oligo’s were cross linked to the slide’s amino silane surface by treating the slide with 600 millijoule of UV irradiation (Stratagene, Cat# Stratalinker 2400). Slides were subsequently backed for 3 hours at 80°C.