title: NIAID Ecab -- E. coli 6.5K
status: Public on Nov 01 2007
contact.city: Bethesda
contact.first: Timothy
contact.middle: G
contact.last: Myers
contact.email: tgm@nih.gov
contact.state: MD
contact.address: 50 South Drive, Room 5509
contact.department: Research Technologies Branch
contact.country: USA
contact.institute: National Institute of Allergy and Infectious Diseases
organism: Escherichia coli
manufacture_protocol: Microarrays are printed with an Omnigrid robotic arrayer by Genomic Solutions (http://www.genomicsolutions.com) that is fitted with a printhead and 16 SMP3 print tips from Telechem (http://www.arrayit.com).
  The E. coli microarray (Ecab) probe set is based on 70mer oligonucleotides from Operon's E. coli Genome Oligo Set Version 1.0 (http://omad.operon.com/download/index.php).
  Oligonucleotide probes are distributed into 384-well polypropylene plates (Genetix, 384 micro array) and dissolved in print buffer (150mM sodium phosphate, pH 8.5, 4% sodium sulfate, 0.0001% N-lauryl sarcosyl) to a normaliz
  The Ecab microarray spot densities are 200 microns (center-to-center).
  The microarrays are printed on Epoxy Slides from Corning (Catalog # 40042) and treated by a method that is an adaptation of the manufacturer's instructions to allow higher throughput processing.