title: Ocimum Biosolutions Yeast OciChip
status: Public on May 13 2010
contact.city: Hyderabad
contact.first: Ocimum
contact.last: Biosolutions
contact.email: govind.a@ocimumbio.com, sandhya.p@ocimumbio.com, hazira.b@ocimumbio.com, anwaruddin.m@ocimumbio.com
contact.state: Andhra Pradesh
contact.address: Banjara Hills Road No., 1 VI Floor Reliance Classic
contact.department: Bioinformatics
contact.country: India
contact.institute: Ocimum Biosolutions
organism: Saccharomyces cerevisiae
description: epoxide
manufacture_protocol: Array Design and Printing
  Yeast OcichipTM  consists of 6368 spots printed on Corning® epoxy coated  glass slides using Omnigrid (Gene Machines). Chip has been printed using 4x4 SMP4 pins on 75.5x 25.5x1.3 mm microarray glass slides with a spot area of 1.8 x3.6 cm. The origin offset values were 0 for X direction and 15000 for Y direction. The dot to dot distance is 290 mm; distance between subarrays is 500 mm in horizontal direction and 200 in vertical direction and no of dots per array is 15x15=225 in subarray 1-16 and 15x12=163 in subarray 17-20. Oligos were printed using spotting buffer A from Ocimum Biosolution at 50% humidity and temperature at 22°C. The time taken by the robotic gridder to print yeast altogether was 16 hrs. Each probe was checked for its performance by standard QC process followed at Ocimum Biosolutions. Printed slides were stored in a light-tight box in a bench-top dessicator, with desiccant and were kept at 4°C until they were used for hybridization.