title: Texas A&M Brucella melitensis 8K
status: Public on May 14 2009
contact.city: College Station
contact.first: Leslie
contact.middle: Garry
contact.last: Adams
contact.email: gadams@cvm.tamu.edu
contact.state: TX
contact.address: 402 Raymond Stotzer Blvd.
contact.department: Veterinary Pathobiology
contact.country: USA
contact.institute: Texas A&M University
contact.phone: 979-845-9816
organism: Brucella melitensis
manufacture_protocol: A set of unique 70-base oligonucleotides representing 3,227 ORFs of B. melitensis strain 16M and unique / divergent genes from B. abortus and B. suis were designed at the Pathogen Expression Core and purchased from Sigma-Genosys (The Woodland, TX). Oligonucleotides were suspended in 3X SSC (Ambion) at the final concentration of 40 uM before robotic arraying in triplicate onto ultraGAPS coated glass slides (Corning) using a Spotarray 72 microarray printer (Perkin Elmer, Downer’s Grove, ILL). Printed slides were steamed and UV cross-linked and stored in a desiccators until use.