title: Chicken Oligo 20.6K 70-mer microarray v3
status: Public on Nov 18 2011
contact.city: Jouy-en-Josas
contact.first: Sylvain
contact.last: Marthey
contact.email: abridge@inra.fr
contact.address: Domaine de Vilvert Bat 320
contact.department: Animal Genetics
contact.country: France
contact.institute: INRA
organism: Gallus gallus
description: Roslin/ARK Cooperative Research Chicken Array set.
  The oligo set (20,673) were designed by ArK using ENSEMBL genes transcripts, the BBSRC fully sequenced set and the DT40 full length sequencing set.
  This oligo set was synthesized by Operon (2007).
manufacture_protocol: Oligo resuspension
  1-1. Oligos from 20K set are arrayed by Operon in 384-well V-bottom plates (Genetix). Each well contains 1 nmol of oligo
  1-2. Resuspend oligos in water (20µM) on Staccato Sciclone (Caliper LS)
  1-3. Incubate at room temperature overnight shaking plates to resuspend DNA
  1-4. Freeze the plates and subsequently thaw them by centrifuging at 1000 rpm for 16 minutes. Centrifuge again at 2000 rpm for 3 minutes
  1-5. Take 10 µl oligos and put it into new polypropylene v-bottom plates (cat# X7022, Genetix)
  1-6. Add 10 µL of 1X Pronto! Universal Spotting Solution (Corning) to each well and mix
  Spotting
  2-1. Centrifuge plates at 2000 rpm for 3 minutes
  2-2. Oligos are ready for spotting
  2-3. Spot onto glass slides (Corning, Ultragaps), with 48 Microspotting pins 946 MP3 on Q-array2 (Genetix). Humidity must be 45-50% and temperature 20-22 °C
  2-4. Fix the oligos slides using UV rays (600 mJoules)
  2-5. Store the slides under vacuum
  2-6. After the print run is completed, cover oligos plates with seals and freeze them at -20°C in a protected environment