title: NRC-IBS_human-mouse_miRNA_422
status: Public on Apr 11 2009
contact.city: Ottawa
contact.first: Brandon
contact.last: Smith
contact.email: brandon.smith@nrc-cnrc.gc.ca
contact.state: Ontario
contact.address: 1200 Montreal Road
contact.department: Institute for Biological Sciences
contact.country: Canada
contact.institute: National Research Council Canada
organism: Homo sapiens
  Mus musculus
description: The Platform data table reflects a condensed representation of the array's replicate features.  The full array layout representing all of the individual features is linked as a supplementary file at the foot of this record.
manufacture_protocol: Oligonucleotide probes (synthesized by MWG-Biotech, High Point, NC and Sigma-Genosys, Saint Louis, MO) were resuspended in Nexterion Spot II buffer (Schott Nexterion, Louisville, KY) at a concentration of 40µM and printed in triplicate onto Nexterion Slide E epoxy-coated glass slides with an Affymetrix 417 Arrayer (Affymetrix, Santa Clara, CA) in a pattern of four 20×18 sub-arrays with 350µm spacing between spots. The spotted “trimeric concatamer” probes were complementary to the mature miRNA sequences concatenated three times, such that a 22 nt miRNA was represented on the array by a 66 nt probe.