title: Lieb Lab at UNC-CH_Yeast Whole-genome Array (PCR-based)
status: Public on Aug 11 2006
contact.city: Chapel Hill
contact.first: Jason
contact.middle: D
contact.last: Lieb
contact.email: jlieb@bio.unc.edu
contact.state: NC
contact.address: 203 Fordham Hall
contact.department: Department of Biology
contact.country: USA
contact.institute: University of North Carolina at Chapel Hill
contact.phone: 919-842-3228
organism: Saccharomyces cerevisiae
description: Whole-genome microarrays with PCR-amplified products represent ORFs, intergenic regions, and other noncoding regions (rDNA, tRNA, transposons, transposon long terminal repeats, telomeres, centromeres, and introns). Generally, each ORF was represented from start codon to stop codon. The intergenic regions consisted of the DNA between annotated ORFs divided such that PCR products were not longer than 1.5 kb, with a few exceptions. The noncoding regions conform to boundaries as annotated by the Saccharomyces Genome Database (SGD) as of the year 2000. Mitochondrial segments did not necessarily conform to annotated functional boundaries. This platform covers several print runs, in which print plates were arranged in different orders.
manufacture_protocol: see Iyer et al. 2001, Nature 409: 533-538