title: PIQOR (TM) custom_583, rat (583)
status: Public on Aug 29 2012
contact.city: Cologne
contact.first: Bernhard
contact.last: Gerstmayer
contact.email: bernhard.gerstmayer@miltenyibiotec.de
contact.address: Stockheimer Weg 1
contact.country: Germany
contact.institute: Miltenyi Biotec GmbH
contact.phone: 0049-221-95048211
organism: Rattus norvegicus
description: The PIQOR (TM) custom Microarray CTID583 is composed of 636 rat specific cDNAs, 99 mouse-specific probes with similarity to rat orthologous sequences and 40 undisclosed probes derived from differential display experiments  These cDNAs include for each array five rat house-keeping genes(ACTA2, ACTB, GAPDH, HPRT, TUBA) as positive controls. In addition four E. coli DNA fragments and the corresponding in vitro transcribed RNAs (spiking controls) provide a control for the labeling and hybridisation efficiency. Two negative controls are given by spotted herring sperm DNA and buffer, respectively. All features are spotted in quadruplicate. The Platform data table reflects a condensed representation of the quadruplicate features.
manufacture_protocol: Defined 200-400 bp fragments of selected cDNAs were generated by RT-PCR, cloned and sequence-verified. Amplified and purified cDNA inserts were spotted in quadruplicate on PIQOR slides using a proprietary piezoelectric spotter. The PIQOR slides were coated in a 2 step process generating a surface which covalently binds DNA molecules.