title: UKD Gastroenterologie Mus musculus oligo array
status: Public on Apr 01 2013
contact.city: Düsseldorf
contact.first: Thor
contact.last: Gehrmann
contact.email: thor.gehrmann@uni-duesseldorf.de
contact.address: Moorenstrasse 5
contact.department: Klinik für Gastroenterologie
contact.country: Germany
contact.institute: Universitätsklinikum Düsseldorf
organism: Mus musculus
manufacture_protocol: 280 mouse oligonucleotide probes and 4 negative controls were designed using OligoWiz and synthesized from Operon (Cologne, Germany). The probes are 70-mers with an average Tm = 79°C and 5’-amino group with a C6 spacer. They were dissolved in Genetix Micro Array Spotting Solution (Genetix, New Milton, UK) to a final concentration of 50 pmol / µl and were printed as four replicates on each slide with a QArray 2 (Genetix, New Milton, UK) at 55% relative humidity and room temperature on Corning UltraGAPS (Corning, Lowell MA, USA). The amino-modified probes were covalently bound to the surface by UV crosslinking (600 mJ) according to the protocol from Corning. The quality of the printing process was proofed with test hybridization using SpotCheck (Genetix, New Milton, UK).