title: CGH-SANGER Homo sapiens 4K 4
status: Public on May 29 2008
contact.city: Leuven
contact.first: Rekin's
contact.last: Janky
contact.email: Nucleomics.Bioinformatics@vib.be
contact.address: Herestraat 49 Box 816
contact.department: Nucleomics Core
contact.country: Belgium
contact.institute: VIB
organism: Homo sapiens
description: The array contains 4066 distinct BAC and PAC sequences. 4008 BACS are from the Sanger institute (Origin = CHORI or SANGER). 58 BACS were provided by K.U.Leuven Department CME (Origin = VIB4). The bacs from SANGER and VIB4 were printed in 2 replicates; the clones from CHORI with 4 replicates.
  The bacs were amplified by DOP - PCR. In total the array design contains 10080 features printed in a layout of 12 meta rows, 4 meta columns, 15 rows per block and 14 columns per block.
manufacture_protocol: MicroGrid Printing Protocol
  Equipment:
  1.	Microgrid (Genomics Solutions, UK).
  2.	384 microtiter plates (X7022, Genetix)
  3.	48 MicroSpot quill pins - 10K (cat# BR001489, Genomics Solutions, UK)
  4.	Codelink slides (cat# 30001100, GE Healthcare).
  Printing 1 array of 12x4 blocks on 1 microscope slide:
  1. Dissolve PCR products in 250mM Phosphate buffer, 0.00025% sarcosyl at concentrations ranging between 300 and 600 ng/ul and array in 384 microtiter plates.
  2. Set spotter settings for 1 array of 12x4 blocks printed with 16 pins: column pitch = 230 um, row pitch = 230 um; Humidity 55-60%; 123 spots per source visit.
  3. Make sure room temperature is between 20-24 degrees Celsius.
  4. Place microtiter plates in the plate hotel with A1 in front left corner.
  5. Place 60 slides on the slide-deck.
  6. Start spotting early in the morning and empty the spotter at the end of the day.
  7. After spotting, incubate the slides in humid chamber with saturated NaCl for 24 to 72 hours.
  8. Block 1%NH4OH, 0.1% SDS, H2O.
  9. Slides can be stored for at least 6 months in a dry-cabinet.