title: WUSTL Brugia malayi v.1 (condensed version)
status: Public on Aug 30 2010
contact.city: Bethesda
contact.first: Sasisekhar
contact.last: Bennuru
contact.email: bennurus@niaid.nih.gov
contact.state: MD
contact.address: 10 Center Dr, Bldg 10, Rm 11N208
contact.department: LPD
contact.country: USA
contact.institute: NIH/NIAID
contact.phone: 301-761-5411
organism: Brugia malayi
description: B. malayi clusters for arrays were selected from 8392 clusters generated and posted in Nembase http://www.nematodes.org/
  These clusters were derived from > 20 cDNA libraries that represent the major B. malayi life cycle stages.  They represent approximately ~40% of the total number of predicted genes for B. malayi.
  Keywords = brugia malayi nematode worm parasite filaria filariasis
  This platform is a condensed version of accession number GPL1483.
manufacture_protocol: Clusters with multiple ESTs, predicted homology by BLAST, and sequence permitting design of a unique 65-mer oligonucleotides were chosen for inclusion on the array.  Oligos were synthesized from the consensus sequence of selected clusters (n=3569) by standard methods by Illumina (San Diego, CA).   The oligonucleotides (50nM in 3x SSC with 0.75M betaine) were printed in duplicate on MWG Epoxy slides (MWG Biotech Inc, High point, NC) by a locally constructed linear servo arrayer (after the DeRisi model, http://derisilabs.ucsf.edu/ ).