title: Cowpea_UP12_expression_array [110706_asp_bean_CB_expr]
status: Public on Nov 26 2014
contact.city: Hangzhou
contact.first: Pei
contact.last: Xu
contact.email: peixu.zaas@gmail.com
contact.address: 198 Shiqiao Rd
contact.department: Vegetable Institute
contact.country: China
contact.institute: Zhejiang Academy of Agricultural Sciences
organism: Vigna unguiculata
description: Each probe is replicated 3 times. Ten copies of each of eight control probes derived from the yeast genome sequence with no matches to the unigene sequences were also included in the microarray.
  A custom Roche NimbleGen microarray was designed based on 29,728 unigene sequences from assembly P12 of cowpea cDNAs in the HarvEST database (http://harvest.ucr.edu/). With the exception of 189 unigenes that failed to pass quality filtering for probe design, each of the unigenes was represented by a set of four 60-mer probes on the fabricated microarray. Due to high sequence similarity, 65 unigenes shared the same group of probes. Therefore, a total of 29,471 unigenes, 26,156 of which were functionally annotated, were represented on the microarray, and a total of 24 microarrays (2 genotypes × 2 tissues × 2 treatments × 3 replicates) were produced for hybridization.
manufacture_protocol: High-density DNA array prepared with Maskless Array Synthesizer (MAS) technology. See manufacturer's website at http://www.nimblegen.com/.