title: ORNL Shewanella oneidensis 11.5k array
status: Public on Feb 15 2006
contact.city: Boston
contact.first: XiaoYun
contact.last: Qiu
contact.email: XiaoYun_Qiu@hms.harvard.edu
contact.state: MA
contact.address: 200 longwood Ave
contact.department: Microbiology and Molecular Genetics
contact.country: USA
contact.institute: Harvard Medical School
contact.phone: 617-432-5098
organism: Shewanella oneidensis MR-1
description: The ArrayIt® brand SuperAmine 2 Microarray Substrates line represents the highest quality amine-containing substrate in the microarray industry. All of our substrates are polished to atomic flatness and manufactured in a state-of-the-art class 100 cleanrooms, with strict controls on particulate, biological and static contaminants, as well as temperature and humidity control.  These pristine glass substrates with reactive amine groups offer quality and performance that cannot be obtained from any other vendor.
manufacture_protocol: The predicted ORFs of Shewanella oneidensis MR-1 (NC_004347) are PCR amplified. The PCR amplicons are deposited onto Telechem Superamine slide (Telechem, Inc., Sunnyvale, Calif.) in duplicate. There are 32 blocks on the array. In each block, there are 18 columns and 20 rows. See detailed description on manufacture protocol in "Global Transcriptome Analysis of the Heat Shock Response of Shewanella oneidensis" (J. Bacteriol. Vol 186, No.22: 7796-7803).