title: Gallus gallus 3k multi-sample microarray platform
status: Public on Dec 29 2007
contact.city: Blacksburg
contact.first: Huifeng
contact.last: Li
contact.email: lihuifengtom@yahoo.com.cn
contact.state: VA
contact.address: 3570 Litton Reaves Hall
contact.department: Animal and Poultry Sciences
contact.country: USA
contact.institute: Virginia Tech
contact.phone: 1-540-231-3183
organism: Gallus gallus
manufacture_protocol: Oligonucleotide probes were synthesized by a company(Sanbiotech, Beijing) and purified by using QIAquick PCR Purification kit (Qiagen, Chatsworth, CA).  After checked on a 1% agarose gel, probes were dissolved in 15µl spotting buffer (50% DMSO) at final concentration of 0.5 ug/ul.  Using SpotBot™ microarrayer (Telechem international Inc, CA), these probes were printed in duplicate on ExprexionsTM slide (GenExprex Corp, NY).  Each slide contains 3072 spots within 48 blocks which contain the same 20 genes and controls at 23°C and 50% humidity.  After being spotted, the slides were snap dried in an 80°C oven for 30 seconds, cross-linked with UV light at 600 mJ in a UVP CL-1000 cross-linker (UVP Inc, Upland, CA), and stored at room temperature.