title: RT² Profiler PCR Array Mouse Fibrosis
status: Public on Jul 03 2023
contact.city: San Diego
contact.first: Tiziano
contact.last: Pramparo
contact.state: CA
contact.address: 3611 Valley Centre Drive
contact.department: R&D
contact.country: USA
contact.institute: Travere
organism: Mus musculus
description: PCR assay
  The Mouse Fibrosis RT² Profiler PCR Array profiles the expression of 84 key genes involved in dysregulated tissue remodeling during the repair and healing of wounds. Wound healing consists of  three phases: inflammation, granulation and tissue remodeling. During tissue remodeling, apoptosis removes myofibroblasts, and the deposited extracellular matrix (ECM) is remodeled to resemble the original tissue. Fibrosis occurs when inappropriate tissue remodeling results in excess ECM deposition due to inappropriate survival of myofibroblasts or lack of ECM proteolytic degradation. The inflammation and enhanced TGFß signaling often present in fibrotic tissues causes cells to differentiate into myofibroblasts via epithelial-to mesenchymal transition (EMT). On the other side of the spectrum, chronic wounds feature dysregulated tissue remodeling with enhanced ECM degradation. This array contains genes encoding ECM remodeling enzymes, TGFß signaling molecules and inflammatory cytokines, as well as additional genes important for fibrosis. Using real-time PCR, you can easily and reliably analyze the expression of a focused panel of genes involved in fibrosis and chronic wounds with this array.
manufacture_protocol: see manufacturer's website