title: MAS bovine BlueChip v3
status: Public on Jan 31 2011
contact.city: Charlestown
contact.first: Petr
contact.last: Vodicka
contact.email: petrvod@gmail.com
contact.state: Massachusetts
contact.address: 114 16th Street
contact.department: Department of Neurology
contact.country: USA
contact.institute: Massachusetts General Hospital
contact.phone: +18579280627
organism: Bos taurus
manufacture_protocol: The custom bovine developmental cDNA microarray (v3) used in this study contains 2,441 ESTs derived from different subtracted libraries.Clones coming from those libraries were used for the preparation of the cDNA microarray slides (Vallée et al. Reproduction, 2009, 138,95-105). The PCR products of  selected clones were purified using Unifilter 384-well purification plates (Whatman, Clifton, NJ) according to the manufacturer's instructions.  Purified PCR products were speedvac-evaporated, resuspended in a mixture of equal parts H2O/DMSO, and spotted on ultra-GAPS glass slides (Corning, Corning, NY) using a VersArray Chip WriterPro robot (Bio-Rad). Each clone is spotted three times on the array, for a total of 9408 spots. Additionally, control spots were added to the cDNA array for hybridization quality control. SpotReport Alien cDNA Array Validation System (Stratagene, La Jolla, CA) was printed as negative controls and a cDNA fragment of the Green Fluorescent Protein (GFP) was used as an exogenous positive control.