title: Rat liver oligo array
status: Public on Jan 28 2005
contact.city: Columbia
contact.first: Sachin
contact.middle: S
contact.last: Bhusari
contact.email: sby7d@mizzou.edu
contact.state: MO
contact.address: 920 East Campus Drive
contact.department: Animal Sciences
contact.country: USA
contact.institute: Univ of Missouri
contact.phone: 573-882-0442
organism: Rattus norvegicus
description: The oligos were obtained commercially from MWG Biotech's Rat liver oligo set. Microarrays were prepared by printing 1,353 oligos, 50 mers in length, representing rat genes expressed in liver on Pan epoxy glass slides (MWG Biotech AG, Ebersberg, Germany). The arrays also contained 10 arabidopsis genes, each of them printed 10 times across the array as external controls (Spot report oligoTM, Stratagene, Cedar Creek, Texas). Blank spots and 3X SSC were used as negative controls. A robotic microarray printer with 16 printing tips was used to spot the oligos. The rat liver oligo arrays have a total of 1,920 spots and are organized in 16 blocks arrayed in four rows and four columns. Each block has 120 spots arranged in 10 rows and 12 columns.
  Keywords = Oligos
  Keywords = Microarray