title: Rosenstiel Fundulus heteroclitus 7K array
status: Public on Apr 19 2011
contact.city: Miami
contact.first: Marjorie
contact.middle: F.
contact.last: Oleksiak
contact.email: moleksiak@rsmas.miami.edu
contact.state: FL
contact.address: 4600 Rickenbacker Causeway
contact.department: Marine Biology and Fisheries
contact.country: USA
contact.institute: University of Miami
organism: Fundulus heteroclitus
manufacture_protocol: Amplified cDNA sequences for approximately 7,000 genes from Fundulus heteroclitus cDNA libraries were spotted onto epoxide slides (Corning) using an inkjet printer (Aj100, ArrayJet, Scotland).  Libraries were made from all 40 stages of F. heteroclitus development, immediately post-hatch whole larvae, and adult tissues.  Each slide contained four spatially separated arrays of ~7,000 spots (genes) including controls.  Average proble length was 1.5 Kb.  All spotted genes were sequenced and represent unique contigs.  Thus, even if multiple sequences were annotated identically, they were treated as different genes.  Multiple sequences with the same annotation do not contig together because: 1) they really are the same gene, but the sequences do not overlap, 2) they represent duplicate genes with different chromosomal locations, or 3) they share a high similarity (and hence are named based on this similarity) but are not the same gene.