title: TUS mouse 22k v1.0
status: Public on Oct 31 2012
contact.city: Noda
contact.first: Hitoshi
contact.last: Sasaki
contact.email: j8312703@ed.tus.ac.jp
contact.state: Chiba
contact.address: 2641 Yamazaki
contact.department: Department of Biological Science and Technology
contact.country: Japan
contact.institute: Tokyo University of Science
organism: Mus musculus
manufacture_protocol: Each cDNA was extracted from cDNA library by Wizard SV 96 Plasmid DNA Purification System (Promega) and amplified by polymerase chain reaction (PCR) using Taq polymerase (Promega). PCR products were purified by isopropanol precipitation and resuspended in 50% DMSO, and spotted to GAPS II Coated Slides (Corning) using Micro Grid II (BioRobotics). After spotting, cDNAs were anchored to slide glasses by heat denaturation at 100°C and UV cross-linking (200 mJ).