title: Rat 27K cDNA array
status: Public on Jul 31 2004
contact.city: Rockville
contact.first: Norman
contact.middle: H
contact.last: Lee
contact.email: nletwin@tigr.org
contact.state: MD
contact.address: 9712 Medical Center Drive
contact.department: Department of Mammalian Genomics
contact.country: USA
contact.institute: The Institute for Genomic Research
contact.phone: 301-795-7585
organism: Rattus norvegicus
description: cDNA arrays were prepared from the TIGR Rat 26K Microarray clone set. The clone set represents 20,465 unique sequences (16,306 unique Tentative Consensus sequences plus 4,159 singleton ESTs based on the current Rat Gene Index Build 11.0; http://www.tigr.org/tdb/tgi/rgi/). Following amplification and purification, PCR amplicons were resuspended at 100-200 nM final concentration in 50% DMSO and printed onto bar-coded UltraGAPS Coated Slides (Corning, #40015.) using an Automated Microarraying System from Brooks Automation, Inc (Chelmsford, MA) equipped with a 48 pin printhead containing Stealth 3 MicroSpotting Pins (Telechem International, Inc.). Print configuration is 48 blocks in a 4 x 12 arrangement. Spot diameters are approximately 90-100 microns. Control reporters were printed in alternating blocks, and included 10 Arabidopsis gene fragments, mouse COT, human COT, salmon sperm DNA, 50% DMSO and poly dA. After printing, DNA was cross-linked to the slides by UV irradiation at 120 mJ/cm2 (Stratalinker UV Crosslinker, Stratagene) and subsequently stored at room temperature in a desiccated chamber until use.