title: DKFZ Operon 27k long oligonucleotide array
status: Public on Aug 04 2004
contact.city: Heidelberg
contact.first: Joerg
contact.last: Schlingemann
contact.email: j.schlingemann@dkfz.de
contact.address: Technologiepark III, Im Neuenheimer Feld 280
contact.department: Molecular Biology (B060)
contact.country: Germany
contact.web_link: http://www.dkfz-heidelberg.de/kompl_genome/index.html
contact.institute: German Cancer Research Center (DKFZ)
contact.phone: +49 6221 424672
organism: Homo sapiens
description: Spotted long oligonucleotide array produced using the Operon Human Genome Oligo Set Version 2.1  (21,329 70mer oligonucleotides, based on build 147 of the Human UniGene database and the Human RefSeq Database, plus 24 controls) and the Operon Human Genome Oligo Set Version 2.1 Upgrade (5,462 70mer oligonucleotides, based on build 13.31 of the Human Ensembl Database). Spotting was performed in duplicates (replicate arrays) with 4x6 SMP3 pins (Telechem) at 18-20 °C and 40-50% RH on epoxysilane coated slides (Quantifoil) using 40 µM oligos in FBNC spotting buffer  (G. Wrobel et al., Nucleic Acids Res 31 e67, 2003). Spot centers were 129 µm apart. DNA adhesion was accomplished by 1 h incubation at 60 °C followed by 240 mJ/cm2 UV irradiation at 254 nm.
  Keywords = spotted
  Keywords = human
  Keywords = long oligonucleotide
  Keywords = oligo
  Keywords = 70mer
  Keywords = Operon