title: UON_Campylobacter oligo array v1
status: Public on Sep 01 2009
contact.city: Leicestershire
contact.first: Amy
contact.last: John
contact.email: asj918@gmail.com
contact.address: Loughborough
contact.country: United Kingdom
contact.institute: University of Nottingham
organism: Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819
  Campylobacter jejuni RM1221
  Campylobacter coli RM2228
manufacture_protocol: The slides prepared for microarray were γ APS coated, A+ bar-coded Schott nexterion slides. The slides were printed according to Campylobacter dense array gal by Microgrid II GOD robot using TAS software version V2.4.03. This had 2500 pins with a 26 x 26 pin grid configuration. The spacing between the spots was 0.165 mm and the slides were rehydrated by incubation at room temperature in a humidity chamber for 30 min followed by a 30 min incubation at 60 ºC to dry. The slides were blocked in a solution containing 100 mM ethanolamine, 1 M Tris (pH 9.0), and 0.1% SDS for 15 min at 50 ºC, then thoroughly rinsed with water and spun dry. The slides contain duplicate sets of oligonucleotides representing the genomes of C. jejuni NCTC11168, RM1221 and C. coli RM2228. The arrays are based on the genomes of C. jejuni 11168, C. jejuni RM1221, C. coli RM2228 along with any miscellaneous genes and plasmids from other strains available in the database. They are composed of synthetic oligonucleotide 70-mers: Tm 71 °C ± 5 °C, location from 3’ end of gene >70 bp, poly (N) tract <8 bp, stem length in potential hairpin <8 bp contiguous bases common to any non-self ORF <20 bp, cross-hyb identity to all other genes <70%.