title: Lactococcus lactis IL1403 (v1)
status: Public on Jul 25 2005
contact.city: Haren
contact.first: Sacha
contact.middle: A.F.T.
contact.last: van Hijum
contact.email: s.a.f.t.van.hijum@rug.nl
contact.address: Kerklaan 30
contact.department: Molecular Genetics
contact.country: Netherlands
contact.institute: University of Groningen
organism: Lactococcus lactis subsp. lactis Il1403
description: Aldehyde slides
manufacture_protocol: Amplicons were designed using GenomePrimer in combination with UniFrag, amplified by Eurogentec (Belgium), purified using Qiagen 96-wells purifying plates. Amplicons (about 10 microL) diluted 2x with ArrayIT 2x spotting buffer, in Genetix 384 wells spot plates, spotted on aldehyde slides (ArrayIt) with a GenPak spotter at about 55 % humidity. Blocking of reactive aldehyde groups by boric anhydride treatment.