title: Ocimum Biosolutions S. pneumoniae OciChip
status: Public on May 13 2010
contact.city: Hyderabad
contact.first: Ocimum
contact.last: Biosolutions
contact.email: govind.a@ocimumbio.com, sandhya.p@ocimumbio.com, hazira.b@ocimumbio.com, anwaruddin.m@ocimumbio.com
contact.state: Andhra Pradesh
contact.address: Banjara Hills Road No., 1 VI Floor Reliance Classic
contact.department: Bioinformatics
contact.country: India
contact.institute: Ocimum Biosolutions
organism: Streptococcus pneumoniae R6
description: epoxide
manufacture_protocol: Array Design and Printing
  S. pneumoniae OcichipTM  consists of 2048 spots printed on Corning® epoxy coated  glass slides using Omnigrid (Gene Machines). Chip has been printed using 4x4 SMP4 pins on 75.5x 25.5x1.3 mm microarray glass slides with a spot area of 1.8x 1.8cm. The origin offset values were 0 for X direction and 10000 for Y direction. The dot to dot distance is 350 mm for x axis and 350 mm for y axis; distance between subarrays is 150 mm and no of dots per array is 12x12=144. Oligos were printed using spotting buffer A from Ocimum Biosolution at 50% humidity and temperature at 22°C. The time taken by the robotic gridder to print yeast altogether was 5 hrs. Each probe was checked for its performance by standard QC process followed at Ocimum Biosolutions. Printed slides were stored in a light-tight box in a bench-top dessicator, with desiccant and were kept at 4°C until they were used for hybridization.