title: Chromosomal alteration study using high resolution BAC array
status: Public on Feb 29 2004
contact.city: Columbus
contact.first: Nehad
contact.last: Mohamed
contact.email: mohamed-1@medctr.osu.edu
contact.state: OH
contact.address: 420W12th Ave
contact.department: Comperhensive Cancer Center/HCG
contact.country: USA
contact.web_link: http://www.dnaarrays.org
contact.institute: The Ohio State University
contact.phone: 614-688-4539
organism: Homo sapiens
description: 36 RPCI-11 BAC clones covering the q-arm of chromosome21 from position15.1MB(close to the centromere)to the telomeric position 46.9 MB with an average gap between clones of 800 kb (range 346-1593 kb)In addition 23 randomly selected clones, each representing 1 of the remaining chromosomes1-20,22,X and YPositions of genes and BAC clones were determined according to the NCBI (http://www.ncbi.nlm.nih.gov/mapview) 59 BAC clones spotted in 6 replicas for a total 354 spots on GAPII corning glass slides using Affymetrix GMS417 Affymetrix Arrayer with 4-ring&pin configuration. (Affymetrix,Santa Clara, CA). Purified clones spotted in 50%DMSO DNA Isolation of these clones was performed from bacterial culture (250ml) using Qiagen midi kit (Qiagen, Valencia CA)
  Platfom_submitter: Nehad Mohamed
  Platform_submitter e-mail: mohamed-1@medctr.osu.edu
  Platform_submitter weblink: www.dnaarrays.org
  Keywords = neoplasia, meyloid leukemia, and  dysplasia, chromosomal alterations