title: Lactococcus lactis transfer RNA array
status: Public on Feb 18 2016
contact.city: Potsdam
contact.first: Paul
contact.middle: Georg
contact.last: Saffert
contact.email: psaffert@gmx.de
contact.state: Brandenburg
contact.address: Karl-Liebknecht Straße 24-25
contact.department: Biochemistry
contact.country: Germany
contact.institute: University of Potsdam
organism: Lactococcus lactis
manufacture_protocol: 1. Oligos were arrayed in 384 well plates with a final concentration of 10 µM in 3× SSC/0.01% SDS in a total volume of 25 µl.
  2. Arrays were printed onto (3-Glycidyloxypropyl)trimethoxysilane (Sigma) activated glass slides using a contact-spotter (QArray 2, Moleculare Devices).
  3. After spotting, arrays were incubated overnight at 4°C in a humidified atmosphere and subsequently dried for 30 min at 30°C.
  4. Arrays were stored in a dry atmosphere at room temperature until use.