title: Chicken cDNA DDMET 1700 array version 1.0
status: Public on May 01 2006
contact.city: Rennes cedex
contact.first: Christian
contact.last: Diot
contact.email: Christian.Diot@rennes.inra.fr
contact.address: 65, rue de saint-Brieuc, CS 84215
contact.department: Animal Genetics
contact.country: France
contact.institute: INRA
organism: Gallus gallus
description: This set includes 195 differential display cDNA products from lean and fat chickens, 128 metabolism specific cDNAs, 2 fluorescent oligonucleotide (TagF and TagR, Eurogentec) control probes and 3 ready-to-spot PCR products from Arabidopsis thaliana (corresponding to RUBISCO activase (RCA), root cap 1 (RCP1) and papain-type cysteine endopeptidase (XCP2), Stratagene) at 3 different concentrations (10, 40 and 80 ng/µl). We spotted all the probes in triplicate, except control probes in duplicate, resulting in 1728 spots.
manufacture_protocol: Probes were suspended in 3X SSC/betaine and printed using MicroGrid II (Biorobotics) arrayer and arrayer software version 2.2.2.8