title: Agilent-020430 Danio rerio custom 8x15k miRNA [Feature Number version]
status: Public on Dec 01 2012
contact.city: Leiden
contact.first: Annemarie
contact.middle: H.
contact.last: Meijer
contact.email: a.h.meijer@biology.leidenuniv.nl
contact.address: Einsteinweg 55
contact.department: Animal Sciences and Health
contact.country: Netherlands
contact.institute: Institute of Biology, Leiden University
organism: Danio rerio
description: Custom-designed Agilent zebrafish 8x15k miRNA platform. The 15k design contained a duplicate of 7604 probes of 60-oligonucleotide length. The probes consisted of 2x22 nucleotide sequences antisense to mature miRNAs separated by a spacer of 8 nucleotides (CGATCTTT) and with a second spacer with the same sequence at the end. From 7604 probes 546 were designed for left (5') and right (3') arms of the hairpins of zebrafish miRNAs  known in miRBase, while the remainder 7058 probes corresponded to predicted hairpin structures in the zebrafish genome that might include additional miRNAs but were not considered in this study
  
manufacture_protocol: the arrays were produced according to manufacturer's protocol, check website: http://www.chem.agilent.com/en-US/products/instruments/dnamicroarrays/pages/default.aspx