title: MWG_Zebrafish_14K
status: Public on Jan 01 2008
contact.city: Taipei
contact.first: Ming Yi
contact.last: Chou
contact.email: eleber@gmail.com
contact.state: Taiwan
contact.address: 128 Sec. 2 Acdemia Rd.
contact.department: Cellular and Organismic Biology
contact.country: Japan
contact.institute: Acdemia Sinica
contact.phone: 886-227899521
organism: Danio rerio
manufacture_protocol: Zebrafish 14K array comprising 14067 zebrafish genes is an important tool for general biomedical research. Oligonucleotides were calculated using 1800 zebrafish gene sequences from the NCBI and a database of 12768 putative open reading frames (ORF). The ORFs were established in-house using NCBI zebrafish EST sequence information.
  The commercial zebrafish 14K oligonucleotides set (MWG
  Biotech AG, Ebersbach, Germany) were obtained and were printed on UltraGAPS Coated slide (Corning, New York, NY ) with use of the OmniGrid 100 microarrayer (Genomic Solutions, Ann Arbor, MI) according to the manufacture’s instructions. The 14,067 oligonucleotides represent 9666 genes (7009 singlet genes and 2657 redundant genes), and the redundancy of this chip is 31 %. The detailed description of the oligonucleotides information can be obtained on the Ocimun Biosolution website (http://www.ocimumbio.com/web/default.asp).