title: Operon Library Version MEEBO Mus musculus v1.05
status: Public on Mar 23 2011
contact.city: New York
contact.first: Yupu
contact.last: Liang
contact.email: liangy@rockefeller.edu
contact.state: NY
contact.address: 1230 York Avenue
contact.country: USA
contact.institute: The Rockefeller University
organism: Mus musculus
description: The supplementary file 'GPL13275_A-MEXP-220.adf.txt' represents the design file for this array.
manufacture_protocol: The Qiagen-Operon (Alameda, CA) mouse 70-mer oligonucleotide library, version 3.0, representing 31,769 annotated genes were suspended in 3X SSC and printed at 22C and 65% relative humidity on aminosilane-coated Cel Associates slides (#CSA-100C, Pearland, TX) using a high-speed robotic OmniGrid machine (GeneMachines; San Carlos, CA) with Stealth SMP3 pins from Telechem (Sunnyvale, CA). Spot volumes were 0.5 nl and spot diameters 75-85 mm. The oligonucleotides were crosslinked to the slide substrate by exposure to 600 mJ of ultraviolet light.