title: CDRI Mycobacterium tuberculosis H37Rv Whole Genome Microarray 4.1k
status: Public on Jul 01 2009
contact.city: Lucknow
contact.first: Ranjana
contact.last: Srivatsava
contact.email: drranjana@yahoo.com
contact.address: Chattar Manzil Palace
contact.country: India
contact.institute: Central Drug Research Institute
organism: Mycobacterium tuberculosis H37Rv
description: Genotypic Designed M.tuberculosis H37Rv Whole Genome Microarray 4.1k
  M.tuberculosis H37RV microarray was custom designed using multiple 60mer oligonucleotides covering all known transcripts of M.tuberculosis H37Rv genome. Array was designed at Genotypic Technology using Agilent Technologies e-Array platform. All 60mer oligo sequences were designed based on the H37Rv sequences available from Sanger, Tuberculist and NCBI databases. Probe validation was done using the following criteria: The 60mer oligonucleotide has to be specific for the H37Rv genome only (Sequence Identity shall be 100% identical only to H37Rv), Average Tm would meet the recommended DNA hybridization standards.  39986 unique probes were designed for 4080 genes at an average of 9 probes per gene. Carefully selected 60mer probes were printed insitu using Agilent’s Inkjet technology.
  The Platform data table reflects a condensed representation of the array's replicate features.  The full array layout representing all of the individual features is linked as a supplementary file at the foot of this record.
manufacture_protocol: Refer to manufacturer website http://www.opengenomics.com