title: Human 11k cDNA arrays
status: Public on Jun 01 2012
contact.city: Brussels
contact.first: Frederick
contact.last: Libert
contact.email: flibert@ulb.ac.be
contact.address: 808 route de Lennik
contact.department: IRIBHM
contact.country: Belgium
contact.institute: ULB
organism: Homo sapiens
description: The cDNAs used for the slides were isolated from cDNA libraries of CP64-MEL melanoma cell line, normal melanocyte, leukocytes and fetal brain (Invitrogen Carlsbad, CA). Slides containing 25K spots with 11K identified cDNA by sequencing were printed.
manufacture_protocol: The cDNA inserts were amplified from replica plate cultures by PCR, purified and mechanically spotted onto Amino-silane coated slides (UltraGAPS, Corning) using a MicroGridII robotic spotter (BioRobotics).