title: Explore HT
status: Public on Sep 22 2025
contact.city: Seoul
contact.first: Jae-ik
contact.last: Oh
contact.email: jae3136@gmail.com
contact.address: 101, Daehak-ro, Jongno-gu
contact.department: Translational Medicine
contact.country: South Korea
contact.institute: Seoul National University College of Medicine
organism: Homo sapiens
manufacture_protocol: Samples and controls are transferred to one sample source plate and samples are diluted. High multiplexed PEATM probes, matched pairs of antibodies with unique DNA oligonucleotides, bind to their respective proteins in the plasma samples. Then oligonucleotides that are brought into proximity hybridize and are extedned using a DNA polymerase. The piece of DNA barcode that is created is then amplified by PCR. Unique sample indexes are added to every sample, to allow pooling of the DNA amplicons for all samples. Samples from the same dilution block are pooled together, resulting in one pool per dilution block, each containing 192 samples/controls. Each pool is purified using magnetic beads. The quality of each Olink Explore HT library is assessed through automated electrophoresis. The Olink Explore HT library is sequenced by NGS using Illumina platform. The relative concentration of each biomarker, based on mathced counts (the number of reads for each specific combination of sample and assay), is calculated using the Olink NPX Explore HT software.  https://7074596.fs1.hubspotusercontent-na1.net/hubfs/7074596/01-User%20Manuals%20for%20website/1293-olink-explore-ht-user-manual.pdf