title: Streptococcus pneumoniae TIGR4/R6 v2
status: Public on Jun 24 2006
contact.city: Haren
contact.first: Sacha
contact.middle: A.F.T.
contact.last: van Hijum
contact.email: s.a.f.t.van.hijum@rug.nl
contact.address: Kerklaan 30
contact.department: Molecular Genetics
contact.country: Netherlands
contact.institute: University of Groningen
organism: Streptococcus pneumoniae
manufacture_protocol: Amplicons were designed using GenomePrimer in combination with UniFrag, amplified by Eurogentec (Belgium), purified using Qiagen 96-wells purifying plates. Amplicons (about 10 microL) diluted 2x with ArrayIT 2x spotting buffer, in Genetix 384 wells spot plates, spotted on aminosilane slides (EZ-Ray) with a GenPak spotter at about 55 % humidity. Amplicons were cross-linked to the glass slide surface by UV-treatment. Slides were cleaned by boric anhydride treatment.