title: Combimatrix 90k TomatArray 1.0
status: Public on Feb 12 2013
contact.city: Portici (NA)
contact.first: Antonio
contact.last: Di Matteo
contact.email: adimatte@unina.it
contact.address: Via Universita' 100 - Parco Gussone - Edificio 75
contact.department: DiSSPAPA
contact.country: Italy
contact.institute: University of Naples Federico II
contact.phone: 00390812539208
organism: Solanum lycopersicum
description: 20200 specific oligos (35-40mer) were designed on Tomato Gene Index Version 11 (LeGI v.11, June21, 2006) tentative consensus (TC) sequences by using the program design OligoArray 2.1 (Rouillard et al. 2003). As negative controls nine bacterial oligonucleotides sequences provided by Combimatrix were utilized. The 90K TomatArrayTM  1.0 was designed with four replicates of each probe randomly distributed across the array to allow measurement of the variability within the array. All the procedures provided were performed as indicated by Combimatrix protocols available at Combimatrix website (www.combimatrix.com)
manufacture_protocol: Probes are directly synthesized on a 90k Combimatrix array by an in situ oligo synthesis system developed by Combimatrix Corporation (Mukilteo, USA) using the Combimatrix CustomArray synthesizer. Standard synthesis protocol provided by Combimatrix was used.