title: MZH_Zebrafish_16k_v1.0
status: Public on Sep 09 2011
contact.city: Nagoya
contact.first: Shin'ichi
contact.last: Akiyama
contact.email: akiyama@med.nagoya-u.ac.jp
contact.state: Aichi
contact.address: 65 Tsurumaicho
contact.department: Department of Nephrology
contact.country: Japan
contact.institute: Nagoya University Graduate School of Medicine
organism: Danio rerio
manufacture_protocol: Zebrafish oligo nucleotide set “Zebra Fish Oligonucleotide Library XEBLIB96-library” was purchased from Sigma aldrich. This oligonucleotide library consists of 16,399 oligos, which represent 16,228 oligos plus 171 control oligos. This is a specially designed oligo set that was selected to best represent all of the genes associated with the public mRNA sequences. These 65-mers have a 5'-C6 amino modification for flexible and universal covalent binding of the oligo to a variety of substrates. The oligonucleotides were spotted on a aminosilane coated glass slide (Matsunami glass Co. Ltd.) by using GENE SHOT arrayer (Nippon Gaishi Co. Ltd.).