title: Species Specific Phosphorylation Array
status: Public on Apr 14 2011
contact.city: Winnipeg
contact.first: Neeloffer
contact.last: Mookherjee
contact.email: mookherj@cc.umanitoba.ca
contact.state: MB
contact.address: 799-JBRC, 715 McDermot Ave
contact.department: Internal Medicine
contact.country: Canada
contact.institute: University of Manitoba
contact.phone: 204-272-3115
organism: Bos taurus
  Ovis aries
description: JPT Peptide Technologies applies its unique PepStar™ peptide microarray platform to generate customized peptide microarrays on glass slides for biomarker discovery, immuno monitoring and detection and validation of protein interactions. In addition, PepStar™ technology also enables characterization of enzyme target families such as kinases, phosphatases and proteases as well as methyl, glycosyl, acetyl transferases and lysine deacetylases.
  Peptides are immobilized on glass slides via a flexible linker. Chemoselective coupling generates microarrays of directed and covalently attached peptides. Multiple identical copies of peptide microarrays can be prepared at unmatched prices and turnaround times. Incubation can be performed manually or automated and read-out is performed via fluorescence. This results in low backgrounds and high sensitivity.
manufacture_protocol: Peptide derivatives (50 nmol) were dissolved in 25 μL of printing solution (70% DMSO, 25% 0.2 M sodium acetate pH 4.5, 5 % glycerol; by
  vol.) and transferred into 384-well microtiterplates. Two droplets of 0.5 nL peptide solution were deposited per spot on aldehyde functionalized glass slides using the non-contact printer Nanoplotter of GESIM (Großerkmannsdorf, Germany) equipped with 8 piezoelectric NanoTips
  (GESIM, Großerkmannsdorf, Germany). Printed peptide microarrays were kept at room temperature for 5 hours, washed with deionised water, quenched for 1 hour with 50% aqueous hydroxylamine, washed extensively with water followed by ethanol, and dried under a stream of nitrogen. Each block of 300 peptides was printed in triplicate on each slide. The final physical dimensions of the array were 19.5 X 19.5 mm with each peptide spot having a diameter of ~350 μm and peptide spots being 750 μm apart.