title: PGA Mouse chip 20K oligo array, version 1.1
status: Public on Jul 31 2011
contact.city: Malmö
contact.first: Harry
contact.last: Björkbacka
contact.email: harry.bjorkbacka@med.lu.se
contact.address: CRC 91:12, UMAS entrance 72
contact.department: Dept. of Clinical Sciences, Malmö
contact.country: Sweden
contact.web_link: http://www.med.lu.se/expcard
contact.institute: Lund University
organism: Mus musculus
manufacture_protocol: The PGA mouse v1.1 oligonucleotide (70-mer) array (Massachusetts General Hospital (MGH), Cambridge, MA) , including 19,549 reporters, were printed at the microarray core (MGH) using an Omnigrid printer (Genemachines Inc., San Carlos, CA) from 384-well polystyrene V-bottom plates (Genetix, Charlestown, MA) to Surmodics slides. Oligos were printed in 150 mM sodiumphosphate pH 8.5 with 0.0005% N-lauroyl sarcosine. Oligos were coupled to the activated slides by allowing the aminolinker on the oligos to react over night in a humidity chamber. Unreacted groups on the slide surface were blocked with 150 mM glycine, 0.1 M tris, 0.1% N-lauroyl sarcosine, pH 9.