title: MPI oyster gigas 9K v1.0
status: Public on Aug 15 2009
contact.city: Plouzane
contact.first: Arnaud
contact.last: Huvet
contact.email: ahuvet@ifremer.fr
contact.address: Ifremer centre de Brest BP 70
contact.department: Physiologie Fonctionnelle des Organismes Marins
contact.country: France
contact.institute: IFREMER
contact.phone: +33 2 98 22 46 93
organism: Magallana gigas
manufacture_protocol: Microarray slides were printed by the Max Planck Institute (Berlin, Germany) with a GMS417 Arrayer (Genetic Microsystems) on poly-L-lysine-coated slides prepared locally according to a standard protocol. Each slide contained a total of 11,088 features spotted in duplicate to reduce positional bias of the fluorescence readout, and divided into 48 blocks of 22 rows and 21 columns each. Replicate spots were located within the same block (i.e. spotted with the same pin) but were never side-by-side. Printed slides were exposed to UV light for cross-linking and blocked in a succinic anhydride/sodium borate solution. Excess DNA probe material was then washed off in a water bath at 95°C. Blocked slides were dehydrated in a bath of 100% ethanol, centrifuged, and stored in a dark, cool, dry location until use.