title: Princeton Microarray Facility AOA Archetype Microarray BC015
status: Public on Nov 15 2012
contact.city: Boston
contact.first: Silvia
contact.last: Newell
contact.email: sen@bu.edu
contact.state: Massachusetts
contact.address: 675 Commonwealth Ave.
contact.country: USA
contact.institute: Boston University
organism: synthetic construct
description: single printing of AOA probes
manufacture_protocol: 1.The probe oligonucleotides are printed onto Corning Ultragaps or Erie Scientific Epoxy Silane slides using a DeRisi style arrayer (DiRisi et al., 1997) and pins from Parallel Synthesis.
  2.The 70-mer oligo probes (Operon Technologies) were adjusted to a concentration of 0.05 mg ml-1 in 50% DMSO and were spotted on CMT-GAPS amino silane-coated glass slides (Corning, Corning, NY).
  3. After printing, the slides were baked at 80°C for 3 h and stored in the dark at room temperature under slight vacuum in a dessicator.