title: Agilent-018073 Custom Brassica 60-mer two-color microarray [Probe Name version]
status: Public on May 08 2015
contact.city: Bethesda
contact.first: GEO
contact.last: admin
contact.email: geo@ncbi.nlm.nih.gov
contact.state: MD
contact.address: 9000 Rockville Pike
contact.country: USA
contact.institute: NCBI/NLM/NIH
organism: Brassica
manufacture_protocol: The assembled Brassica sequences (94,558 instances including those represented in both orientations) were submitted to Agilent Technologies’ eArray web portal for gene expression probe design. Probes with a base composition score greater than or equal to 3 were omitted from the final design; resulting in a total of 91,854 unique probes (including 6,989 derived from oppositely oriented pairs of sequences) that were included in the microarray design, of which 10,466 were predicted to have cross-hybridization potential.
  11,893 probes were randomly selected to be represented in duplicate in the final design, which also included Agilent Technologies’ standard panel of quality control and spike-in probes.
  Microarrays were manufactured using Agilent Technologies’ SurePrint Technology in the 2x104k format (two arrays containing ~104,000 probes on a single 1” x 3” glass slide).