title: NanoString nCounter Human miRGE Expression Assay Custom CodeSet
status: Public on Oct 24 2016
contact.city: Sacramento
contact.first: Clifford
contact.middle: G.
contact.last: Tepper
contact.email: cgtepper@ucdavis.edu
contact.state: CA
contact.address: 4645 2nd Avenue, Room 2300A
contact.department: Biochemistry and Molecular Medicine
contact.country: USA
contact.institute: UC Davis School of Medicine
contact.phone: 916-734-7195
organism: Homo sapiens
description: NanoString nCounter Analysis System: Hybridization-based target capture, imaging, and counting technology utilizing digital color-coded barcodes.
manufacture_protocol: A NanoString nCounter miRGE CodeSet (miX_H_TP53) was custom-designed to enable the expression profiling of 105 TP53-associated transcripts (96 mRNAs, 10 miRNAs) plus 5 normalization control transcripts. Complete details of the gene targets and probe sequences are presented. The technology employs gene-specific CodeSets consisting of target-specific capture and reporter probes 35-50 bases in length. The reporter probes contain molecular, color-coded barcodes that afford high sensitivity and capability for high multiplexing. Targets were selected on the basis of their involvement in the canonical p53 signaling network as being either direct transcriptional targets of p53, known modulators of p53 function, and/or genes altered in response to TP53 mutation and loss/gain-of function.