title: HMC Human 29k Array
status: Public on Jun 27 2013
contact.city: Odense C
contact.first: Martin
contact.middle: Jakob
contact.last: Larsen
contact.email: martin.larsen@rsyd.dk
contact.address: Sdr. Boulevard 29
contact.department: Dept. of Clinical Genetics
contact.country: Denmark
contact.institute: Odense University Hospital
organism: Homo sapiens
manufacture_protocol: For fabrication a human oligonucleotide library consisting of 28,919 DNA oligonucleotide probes (60-mer) was used (Compugen-Sigma-Genosys, The Woodlands). The oligonucleotide probes were solubilized in 150 mM sodium phosphate buffer (pH 8.5) to a final concentration of 20 pmol/µl and spotted onto CodeLink HD (SurModics) activated glass slides by a high-precision spotting robot (Virtek ChipWriter Pro, ESI). The slides have an active polymer coating of amine-reactive groups permitting the 5?-amino modified DNA oligos to covalently attach under high relative humidity. The microarray fabrication was carried out in a controlled environment at 38% humidity and the temperature was held constant at 23 °C. SMP 2.5 stealth pins (TeleChem Interational) was used to deposit the oligos onto surface of the slides. Up to 85 arrays were spotted per batch. After printing, slides were incubated overnight at 70% humidity and blocked as recommended by the manufacturer.