title: [SBRI/Sherman]-Mtb-10368-v1.0
status: Public on Jul 28 2010
contact.city: Seattle
contact.first: Ashley
contact.middle: M
contact.last: Sherrid
contact.state: WA
contact.address: 307 Westlake Ave N
contact.country: USA
contact.institute: Seattle Biomedical Research Institute
organism: Mycobacterium tuberculosis
manufacture_protocol: 1.Qiagen array ready oligo set version 1.0 are arrayed in 384-well V-bottom plates. Each well contains 600 pmol of 70-mer oligo.
  2. Resuspend oligos in 3X SSC to 40 uM
  3. Freeze plates at -20°C when not in use.
  4. Before printing,thaw at 4°C. This can be done the night before a print run is started.
  5. Seal plates with Corning seals.
  6. Spin for 1 minute at 1000 RPM to settle condensation.
  7. One plate at a time, load into microarray robot.
  8. Load gal file based on Qiagen provided dataset.
  9. Cover with Corning seals and refreeze oligos after print run is complete.