title: BCBC Mouse PancChip 4.0 Array
status: Public on Feb 18 2010
contact.city: Philadelphia
contact.first: Chiao-Feng
contact.last: Lin
contact.email: chiaolin@pcbi.upenn.edu
contact.state: PA
contact.address: 1413 Blockley Hall, 423 Guardian Dr.
contact.country: USA
contact.institute: Penn Center for Bioinformatics
organism: Mus musculus
description: array containing probes representing mostly genes expressed in the pancreas
manufacture_protocol: Bacterial clones were grown to confluence overnight in flat-bottom 96-well square block plates with a volume of 1 ml LB-Amp medium per well. Plasmid DNA was prepared for each clone using a Qiagen 3000 robot, and the purified DNA was used as a template to amplify the inserts with PCR. The clones were assigned a PCR score of pass indicating a single strong band and fail if there were no bands or multiple bands. All the PCR products were purified, eluted with deioinzed sterile water, diluted with an equal volume of DMSO (Sigma) and printed on poly-L-lysine-coated slides with a Biorobotics Microgrid II. The PancCHip 4.0 contains the 3,840 elements of PancChip 2.0 plus 7226 clones derived from the mouse Consorium libraries plus various control spots.