title: TUG/IGB-hsa-29.55K
status: Public on Jun 05 2015
contact.city: Vienna
contact.first: Michael
contact.last: Karbiener
contact.email: michael.karbiener@takeda.com
contact.address: Benatzkygasse 2-6 / G
contact.department: Global Pathogen Safety
contact.country: Austria
contact.institute: Takeda
organism: Homo sapiens
manufacture_protocol: The human oligonucleotide probe set (MWG) consisted of 29550 50-mer DNA oligonucleotides, enabling the detection of 15539 distinct RefSeq mRNAs, as well as non-RefSeq-annotated transcripts (provided by the manufacturer). Lyophilized oligonucleotides (in 384-well plates) were dissolved in 3x SSC (prepared from 20x stock consisting of 3 M NaCl and 300 mM trisodium citrate, pH 7) / 1.5 M betaine (20 µl / well) and spotted in a 4 x 12 print tip group pattern on epoxy-coated glass slides (Schott Nexterion, Peqlab #39-1064016). For fixation, slides were baked at 120°C for 1 h.