title: Meyerowitz Lab Arabidopsis Flower-specific cDNA Array
status: Public on May 02 2004
contact.city: Pasadena
contact.first: Frank
contact.last: Wellmer
contact.email: wellmer@caltech.edu
contact.state: CA
contact.address: 1200 E. California Blvd.
contact.department: Biology 156-29
contact.country: USA
contact.institute: California Institute of Technology
contact.phone: 626-395-8438
organism: Arabidopsis thaliana
description: Elements for the flower-specific cDNA microarray were obtained from three different sources. First, for those genes that are known or suspected to be involved in flower development, we either amplified fragments of their transcribed regions from cDNA or, if available, obtained cDNA clones from EST clone collections. Second, we generated cDNA libraries using RNA extracted from different floral tissues. For all libraries, the RNA preparations were subtracted with leaf RNA to reduce the abundance of clones representing ubiquitously expressed genes and thus, to enrich for flower-specific transcripts. The identity of most of the library clones was not determined prior to their use for preparing the array. Lastly, we added a set of 2632 non-redundant clones from a non-subtracted flower-derived library.
  The array is comprised of 10,816 elements representing approximately 5000-6000 genes.
  Keywords = Flower
  Keywords = cDNA array