title: Microarrays of human nuclear encoded tRNAs
status: Public on Apr 20 2019
contact.city: Oxford
contact.first: Irina
contact.last: Chelysheva
contact.email: irina.chelysheva@ndm.ox.ac.uk
contact.address: Roosevelt Dr
contact.department: NDM
contact.country: United Kingdom
contact.institute: University of Oxford
organism: Homo sapiens
manufacture_protocol: 1. Oligos were arrayed in 384 well plates with a final concentration of 10 µM in 3× SSC/0.01% SDS in a total volume of 25 µl.
  2. Arrays were printed onto (3-Glycidyloxypropyl)trimethoxysilane (Sigma) activated glas slides using a contact-spotter (QArray 2, Moleculare Devices).
  3. After spotting arrays were incubated overnight at 4°C in a humidified atmosphere and subsequently dried for 30 min at 30°C.
  4. Arrays were stored in a dry atmosphere at room temperature until use.