title: UC Human 22K v2.2 Operon 70-mer oligo array
status: Public on Sep 24 2005
contact.city: Chennai
contact.first: Rajkumar
contact.last: T
contact.email: rajkumart@yahoo.com
contact.state: Tamilnadu
contact.address: Adyar
contact.department: Molecular Oncology
contact.country: India
contact.institute: Cancer Institute (WIA)
contact.phone: +91-44-22350340
organism: Homo sapiens
description: Human 70-mer oligo library (V2.2):  Representing a total of 22,102 annotated genes.
manufacture_protocol: Aminosilane-coated slides (Cell Associates, Cat#CSA-100 C) are used.
  Printing
  1. The Operon 70-mer oligonucleotides were  suspended in 3XSSC.  The DNA is printed on aminosilane-coated slides (Cell Associates, Cat.# CSA-100 C).
  
  UV-Crosslinking and storage
  1. Lay slides array-side-up in a Stratalinker and irradiate at 6000 (x100) mjoules (600mJ).
  2. Store slides at room temperature under vacuum.
  Notes:  The 70 mer oligonucleotides are spotted at 22ºC and 65-85% relative humidity at ~100 mm center-spacing using a high-speed robotic machine (OmniGrid-100; GeneMachines; San Carlos, CA) using Stealth pins (SMP3 pins) from TeleChem (Sunnuvale, CA), mounted on a 48-pin head.  Spot volumes are 0.5 nl and spot diameters are 75-85 mm.