title: Escherichia coli O157:H7 EDL933 Spotted PCR Array
status: Public on Mar 31 2009
contact.city: Ames
contact.first: Chris
contact.last: Minion
contact.email: fcminion@iastate.edu
contact.state: IA
contact.address: 1130 Vet Med
contact.department: VMPM
contact.country: USA
contact.institute: Iowa State University
contact.phone: 515-294-6347
organism: Escherichia coli O157:H7 str. EDL933
description: Corning UltraGAPS substrates (catalog #40016)
manufacture_protocol: The Escherichia coli O157:H7 EDL933 microarray consists of PCR products (100-500 bp) spotted to Corning UltraGAPS™ substrates. Each probe was printed in duplicate, in non-adjacent spots to reduce non-uniform substrate errors in replicated probes. The array consisted of 48 subarrays, with a 15-column x 15-row configuration.