title: CDMC_Drosophila_12k1
status: Public on Oct 01 2004
contact.city: Mississauga
contact.first: Zak
contact.last: Razak
contact.email: z.razak@utoronto.ca
contact.state: ON
contact.address: 3359 Mississauga Road
contact.department: Zoology Department
contact.country: Canada
contact.web_link: http://www.flyarrays.com
contact.institute: University of Toronto
contact.phone: 905-569-4664
organism: Drosophila melanogaster
description: The 12k1 platform is a primarily cDNA-based glass microarray. The array features 11,018 Berkeley Drosophila Genome Project cDNAs, 297 NIH Testis cDNAs (Andrews et al., 2000) and 432 gene sequences that were amplified from genomic DNA.  Approximately 10,500 unique genes are represented by the above, corresponding to roughly 78% of the predicted genes in Drosophila melanogaster (FlyBase annotation release 3.2).  This array is produced by the Canadian Drosophila Microarray Centre located at the University of Toronto.  It is available for distribution to academic labs.
  
  
  The 12k1 arrays are printed on CMT-UltraGAPS substrates (Corning Inc, Acton, MA) using a SpotArray 72 microarrayer (Perkin Elmer, Boston, MA).  There are 26880 spots and 768 blanks on each array in a 12 row by 4 column arrangement of blocks.  Each block contains 560 spots and 16 blanks arrayed in a 24 row by 24 column grid.  The blanks occupy positions 9 through 24 of the 24th row.  The blocks are spaced at 4500 micron intervals and the spot-to-spot distance is 180 microns.  The nominal spot diameter is 125 microns.  Printing conditions and post-print treatments are as described earlier (Neal et al., 2003).
  Keywords = CDMC
  Keywords = Drosophila
  Keywords = microarray
  Keywords = cDNA