title: UI Squid EST 30K cDNA array
status: Public on Jun 02 2006
contact.city: Iowa City
contact.first: Bartley
contact.middle: Joseph
contact.last: Brown
contact.email: bbrown@eng.uiowa.edu
contact.state: IA
contact.address: 5317
contact.country: USA
contact.institute: The University of Iowa
contact.phone: 319 335 6432
organism: Euprymna scolopes
description: Glass slide with 30,005 spots (13,978 unique cDNA clone inserts) in 48 blocks consisting of 25 columns and 25 rows.  Blocks are arranged in 12 rows and 4 columns.  The set of 13,978 cDNA clone inserts was identified form the University of Iowa EST collection.  The clone inserts were amplified with T7 and T3 primers and printed with 50% DMSO on UltraGaps slides (Corning) using a ProSys 5510 Microarray Printer.
manufacture_protocol: The clone inserts were amplified directly from bacterial cell cultures with T7 and T3 primers, purified, dried, resuspended in water and printed with 50% DMSO on UltraGaps slides (Corning) using a ProSys 5510 Microarray Printer.  The 16 control spikes were printed by each pin, at the beginning or at the end of the pin printing area.  The spotted slides were cross-linked and stored in a desiccator.