title: University of Heidelberg custom made sulfur 5.4K array
status: Public on Mar 12 2010
contact.city: Heidelberg
contact.first: Markus
contact.last: Wirtz
contact.address: Im Neuenheimer Feld 360
contact.department: Molecular Biology of Plants
contact.country: Germany
contact.web_link: http://www.hip.uni-heidelberg.de
contact.institute: University of Heidelberg
organism: Arabidopsis thaliana
manufacture_protocol: 50mer oligonucleotides with an N-terminal amino linker for 920 selected genes were optimized and tested for minimal cross-hybridization with the Smith-Waterman alignment and synthesized by Ocimum Biosolutions. Oligonucleotides were diluted to a concentration of 0.01mM to 1mM in 5xSSC (0.75M sodiumchloride and 0.075M sodium citrate) containing 2.5 M betaine and spotted on epoxy-coated Nexterion E slides (Peqlab) with a MicroGrid robot (Biorobotics) using SMP3 pins (TeleChem International). A microarray contains all probes in four replicates.