title: NimbleGen Bos taurus Whole-genome CGH 385k Array (Btau_4.0 version)
status: Public on Nov 09 2010
contact.city: Bologna
contact.first: Pier Luigi
contact.last: Martelli
contact.email: gigi@biocomp.unibo.it
contact.address: via Irnerio 42
contact.country: Italy
contact.web_link: http://www.biocomp.unibo.it
contact.institute: University of Bologna
organism: Bos taurus
description: A cattle genome array for cross species experiment (Goat, Sheep).
  
  Custom tiling arrays designed on the bovine (Bos taurus) genome, Btau_4.0 version, including a fraction of BTA13 of the University of Maryland (UMD) Bos taurus v. 2.0 assembly (ftp://ftp.cbcb.umd.edu/pub/data/Bos_taurus/Bos_taurus_UMD_2.0/). Arrays contained ~385,000 probes on a single slide to provide an evenly distributed coverage with an average interval of ~6 kb for the Btau_4.0 genome. The BTA13 of the UMD v. 2.0 assembly was included from nucleotide position 48 M bp to nucleotide position 78 M bp (4,673 oligonucleotides and average spacing of ~6 kb). This chromosome region was analysed as internal control because it contains the ASIP gene, not assembled in the BTA13 of the Btau_4.0 version.
manufacture_protocol: See manufacturer's website at http://www.nimblegen.com/.
  Probes were designed on the Btau_4.0 version of the Bos taurus genome and on the UMD version 2.0 for part of chromosome 13.
  NimbleGen manufactures high-density DNA microarrays using its proprietary Maskless Array Synthesizer (MAS) technology. At the heart of the system is a Digital Micromirror Device (DMD) that uses a solid-state array of miniature aluminum mirrors to create “virtual masks” that replace the physical chromium masks used in traditional arrays. The DMD directs a pattern of UV light projected onto a microscope slide, which when coupled with UV-mediated DNA synthesis in a parallel, combinatorial manner, can generate up to 2.1 million unique probes on a single microarray.