title: Streptomyces griseus IFO13350 13K KG
status: Public on Aug 31 2007
contact.city: Bunkyo-ku
contact.first: Yasuo
contact.last: Ohnishi
contact.email: ayasuo@mail.ecc.u-tokyo.ac.jp
contact.state: Tokyo
contact.address: 1-1-1 Yayoi
contact.department: Biotechnology
contact.country: Japan
contact.institute: University of Tokyo
organism: Streptomyces griseus
manufacture_protocol: Oligonucleotides are consolidated into 384-well plates, lyophilized by speed-Vac, and resuspended in 3x SSC to a printing concentration of 30uM. Oligos are printed on aminosilane slides. Microspot 10K quill pins (Biorobotics) in a 48-pin tool were used to deposit <0.5 nL per spot onto the slide. The resulting microarrays have a 8 x 4 subgrid layout with 420 spots per subgrid, each spot having approximate diameter and pitch of 90 µm and 190µm, respectively. All of oligo mixture as a positive control was printed in subgrid corners to assist in placing on the grid. The slides were crosslinked in a UV Stratalinker 2400 (Stratagene) at 300mJ. Array quality was assessed by visual inspection and hybridization of representative slides from a print run by dye-labelled random 9-mer oligonucleotides. The quality control images were aquired via the ScanArray Express from Perkin Elmer at a 10 micron resolution and quantified with the Imagene 6.0 software suite.