title: Micro-CRIBI Human Oligo Array (Operon V2.0)
status: Public on Oct 04 2010
contact.city: Chieti
contact.first: Florina 
contact.last: Raicu
contact.email: florina_raicu@yahoo.com
contact.state: Abruzzo
contact.address: Via Colle dell'Ara
contact.department: Aging Research Center (CESI)
contact.country: Italy
contact.institute: G. d’Annunzio University Foundation Chieti
contact.phone: 00390871541479
organism: Homo sapiens
manufacture_protocol: This set consists of 21,329 70mer oligonucleotides (Operon version 2.0) designed on Human Unigene clusters, mainly in the 3 end terminal region. Each oligonucleotide is spotted by our robotic station Biorobotics Microgrid II, in two replicates on a MICROMAX glass slide SuperChip I (Cat No MPS696) provided by PerkinElmer Life Sciences Inc. The deposition is qualitatively assessed by a scan of the spotted slide at 10 micron resolution, using a GSI Lumonics scanner and a protocol that allows the detection of spotted features through the salts derived from the dried solution in which oligonucleotides are dissolved. Microarray slides that meet the quality standards, are washed as recommended by the supplier and the glass slides are stored, ready for use, in the dark under deep vacuum.
  Keywords = Oligo
  Keywords = probe 70mer
  Keywords = MicroCribi
  Keywords = probe set 21.329