title: Custom microRNA microarray Design 4343
status: Public on May 22 2009
contact.city: Baltimore
contact.first: Joshua
contact.middle: T
contact.last: Mendell
contact.email: jmendell@jhmi.edu
contact.state: MD
contact.address: 733 N. Broadway, BRB 471
contact.department: Institute of Genetic Medicine
contact.country: USA
contact.institute: Johns Hopkins University School of Medicine
contact.phone: 410-502-0428
organism: Homo sapiens
  Mus musculus
  Rattus norvegicus
manufacture_protocol: Combimatrix CustomArray™ uses a specially modified “CMOS” semiconductor to direct the molecular assembly of a specific sequence of DNA bases in response to a digital command. Each feature on the array (a microelectrode) is digitally addressed to selectively generate acid, by means of an electrochemical reaction which, in-turn, controls the detritylation reaction during phosphoramidite synthesis. A software program is used to control the microelectrodes, and thus, the detritylation pattern applied on the chip during synthesis.