title: miRFishes_20_LCB_131125-F
status: Public on Jun 30 2016
contact.city: Guangzhou City
contact.first: Zhang
contact.last: Xiujuan
contact.email: zhangxj67@gdei.gd.cn
contact.state: Guangdong Province
contact.address: 105 Xingang West Road, Haizhu District
contact.country: China
contact.institute: Guangdong Entomological Institute
contact.phone: 020-89100920
organism: Acipenser sturio
manufacture_protocol: Arrays are made based µParaflo microfluidic technology (Atactic Technologies). On the µParaflo microfluidic chip, each detection probe consists of a chemically modified nucleotide coding segment complementary to target microRNA (from miRBase, http://microrna.sanger.ac.uk/sequences/) or other RNA (control or customer defined sequences) and a spacer segment of polyethylene glycol to extend the coding segment away from the substrate. The detection probes are made by in situ synthesis using PGR (photogenerated reagent) chemistry.