title: Umea Lucidea array
status: Public on May 21 2011
contact.city: Umeå
contact.first: Mattias
contact.last: Landfors
contact.address: Umeå University
contact.department: Clinical bacteriology
contact.country: Sweden
contact.institute: Umeå University
organism: Pinus sylvestris
  Mus musculus
description: The in-house produced cDNA-array contains 44 clones: 19 clones from mice, 4 clones from Pine (involved in photosynthesis) and 21 artificial clones from the Lucidea Universal ScoreCard (Amersham Bioscienses), where each clone was printed 480 times in 48 identically designed sub-grids.
manufacture_protocol: The clones were dissolved in 50% DMSO and printed using 2500K pins (Biorobotics) with a MicroGrid II 610 instrument (Biorobotics) on Ultra GAPS slides (Corning).  The array is spotted in 48 subarrays (4x12) with a 21x21 pattern within each subarray