title: ZB/SBS-NTU Plasmodium falciparum 3D7 11.0K v1.1
status: Public on Jun 08 2011
contact.city: Singapore
contact.first: Bernardo
contact.middle: J
contact.last: Foth
contact.email: bfoth@gmx.net, ZBozdech@ntu.edu.sg
contact.address: 60 Nanyang Drive
contact.department: SBS
contact.country: Singapore
contact.institute: Nanyang Technological University
organism: Plasmodium falciparum
description: This platform is identical to platform GPL7493 except that 589 oligonucleotides have been added to the platform described here. These additional oligonucleotides represent variable genes (var, rifin) specific to strains Dd2 and HB3.
manufacture_protocol: Long oligonucleotides were selected for P. falciparum 3D7 using the program OligoRankPick, described in Guangan Hu, Manuel Llinás, Jingguang Li, Peter Rainer Preiser & Zbynek Bozdech: Selection of long oligonucleotides for gene expression microarrays using weighted rank-sum strategy. BMC Bioinformatics 2007, 8:350. The 70-bp oligonucleotides were spotted onto poly-L-lysine-coated microscopic slides as described in Zbynek Bozdech, Jingchun Zhu, Marcin P. Joachimiak, Fred E. Cohen, Brian Pulliam & Joseph L. DeRisi: Expression profiling of the schizont and trophozoite stages of Plasmodium falciparum with a long-oligonucleotide microarray. Genome Biology 2003, 4:R9.