title: Texas State University/Ron Walter Oryzias latipes 8k array (version 1)
status: Public on Oct 23 2009
contact.city: San Marcos
contact.first: Ziping
contact.last: Zhang
contact.email: Ziping.Zhang@txstate.edu
contact.state: TX
contact.address: 601 University Drive/Cent. 419
contact.department: Chemistry & Biochemistry
contact.country: USA
contact.web_link: http://www.xiphophorus.org/
contact.institute: Texas State University
contact.phone: 512-245-0358
organism: Oryzias latipes
manufacture_protocol: Medaka expressed sequence tags (ESTs) were downloaded from NCBI GenBank (http://www.ncbi.nlm.nih.gov/). 60-mer oligonucleotides were designed for unigenes using commercial software, ArrayDesigner v3 (Telechem, Sunnyvale, CA, USA) and synthesized by IDT™ (Integrated DNA Technologies, Coralville, IA, USA). The oligonucleotides were diluted in 3×SSC (20×SSC: 3 M NaCl, 0.3 M sodium citrate pH 7.0) and spotted on aminosilane coated Superchip glass slides (Cat no. C18-5131-M20, Erie Scientific, Portsmouth, NH, USA) using a microarraying robot Virtek Chip Writer Pro (Virtek Vision, Waterloo, Ontario, Canada) with 16 Telechem Stealth pins (Cat no.: 946MP3, Telechem). These pins were designed with uptake channels and flat tips, and thus could effectively avoid "donut" spots. The slides were printed in the Genomic Analysis and Technology Core of the University of Arizona (Dr. Ryan Sprissler).