title: PIQOR 6k rat set1
status: Public on Jul 28 2006
contact.city: Cologne
contact.first: Bernhard
contact.last: Gerstmayer
contact.email: bernhard.gerstmayer@miltenyibiotec.de
contact.address: Stockheimer Weg 1
contact.country: Germany
contact.institute: Miltenyi Biotec GmbH
contact.phone: 0049-221-95048211
organism: Rattus norvegicus
description: The PIQOR 6k rat set 1 includes the two arrays PIQOR 348 and PIQOR 350 with a total number of 5957 rat specific cDNAs. These cDNAs include for each array six house-keeping genes as positive controls. In addition four E. coli DNA fragments and the corresponding in vitro transcribed RNAs (spiking controls) provide a control for the labeling and hybridisation efficiency. Two negative controls are given by spotted herring sperm DNA and buffer. The PIQOR array 348 contains 3000 cDNAs and controls spotted in duplicate. The PIQOR array 350 contains 2937 cDNAs and controls spotted in duplicate and 20 cDNAs  as sixfold replicates.
manufacture_protocol: Defined 200-400 bp fragments of selected cDNAs were generated by RT-PCR, cloned and sequence-verified. Amplified and purified cDNA inserts were spotted in quadruplicate on PIQOR slides using a proprietary piezoelectric spotter. The PIQOR slides were coated in a 2 step process generating a surface which covalently binds DNA molecules.