title: Scoelicolor_UMN_Def_v1.0
status: Public on Jun 08 2007
contact.city: Minneapolis
contact.first: Karthik
contact.middle: P
contact.last: Jayapal
contact.email: jayapal@cems.umn.edu
contact.state: MN
contact.address: 421 Washington Ave SE
contact.department: Chemical Engineering and Materials Science
contact.country: USA
contact.institute: University of Minnesota
organism: Streptomyces coelicolor
manufacture_protocol: The following text is taken from the authors' previous publication - Mehra et al., "A framework to analyze multiple time series data: a case study with Streptomyces coelicolor", J Ind Microbiol Biotechnol, 2006 Feb;33(2):159-72.
  "A whole genome cDNA microarray was constructed for
  S. coelicolor. Gene-specific probes with an average
  length of 500 bp were designed based on the genomic
  sequence database at Sanger Center to represent the
  7,825 genes. The primers to amplify these probes were
  designed to be 20 bp in length with a melting temperature
  around 62 deg C. An iterative primer/probe design
  process was adopted. The primers were first Blasted
  against the entire genome to check for the specificity to
  the corresponding genes. The probe sequences resulting
  from these primers were also blasted against the entire
  genome to check for possible cross-hybridization with
  sequences other than the corresponding gene. Any primer
  pair that may give rise to a potentially croshybridizing
  probe sequence was redesigned with alternate
  coordinates. This probe checking-redesign process
  minimized the possibility of cross-hybridization between
  a probe and other cDNA species. For some highly
  homologous genes, a unique probe was not attainable
  and a multigene probe was used. A multigene probe was
  designed in such cases. 7,581 genes were successfully
  amplified and spotted on the microarray. The microarray
  was fabricated on poly-L-lysine slides with duplicate
  spots for each probe."