title: Agilent-024519 RBD_v3_244K_Hughes
status: Public on May 09 2013
contact.city: Toronto
contact.first: Timothy
contact.last: Hughes
contact.state: Ontario
contact.address: 160 College Street
contact.department: Donnelly Centre
contact.country: Canada
contact.institute: University of Toronto
organism: synthetic construct
description: This custom microarray consists of probes representing two complete sets of all possible 10-base sequences. The probes on the array are made double-stranded, ssDNA are stripped from the array, and PCR-amplified to create a dsDNA pool. This DNA pool is then used as a template for in vitro T7 RNA polymerase-mediated RNA transcription to generate an RNA pool used for pull-down experiments using GST-tagged RNA binding proteins. The RNAs pulled-down by each RNA-binding protein are then labelled with a fluorophore and hybridized back onto the array, along with the total pool.
manufacture_protocol: Please refer to the manufacturer's web site at http://www.agilent.com/