title: Tor Vergata Mus musculus 200 22q11Ds chip
status: Public on Apr 11 2006
contact.city: Roma
contact.first: Francesca
contact.last: Amati
contact.email: amati@med.uniroma2.it
contact.address: Via Montpellier 1
contact.department: Biomedicine and Prevention
contact.country: Italy
contact.institute: University of Rome Tor Vergata
contact.phone: +39-6-72596080
organism: Mus musculus
manufacture_protocol: Each oligonucleotide probe was diluited in a spotting buffer (Corning, Schiphol-rijk, The Netherlands) at a final concentration of 50 pmoli/ul. After this, oliigonucleotide probes were mechanically “spotted” onto UltraGAPS glass slides (Corning, Schiphol-rijk, The Netherlands) using The RoboArrayer™ (Microgrid Compact Plus, BioRobotics ).
  Each oligonucleotide probe was represented in triplicate per array. Printing conditions were humidity among 50-55% and temperature 25°C. Printed slides were dried overnight and cross-linked with UV light at 600 mJ using a Strata-linker 2400 (Stratagene, Glenville, VA) and stored in a desiccator at room temperature.