title: Citrus 22k cDNA microarray v1
status: Public on Jul 10 2009
contact.city: Valencia
contact.first: Miguel
contact.last: Perez-Amador
contact.email: mpereza@ibmcp.upv.es
contact.state: Valencia
contact.address: Avda. de los Naranjos s/n
contact.country: Spain
contact.institute: Inst. Biologia Molecular y Celular de Plantas (IBMCP-CSIC)
contact.phone: 34-963877872
organism: Citrus
manufacture_protocol: cDNA clones being the best representative for each superunigene were selected to be PCR-amplified in a final volume of 100 μL using 4 ng of plasmid template, 400 nM of each primer, and 200 μM dNTPs. The reaction products were analyzed by agarose gels, and purified using the Multiscreen-PCR 96-well Filtration System (Millipore). Only PCR reactions yielding single bands were transferred to printing plates, at a final concentration of 150 ng/μL in PRONTO Universal Spotting Buffer (Corning Life Sciences). PCRs were printed onto UltraGAPS aminosilane Corning slides, using a MicroGrid II arrayer (Genomic Solutions). Printed slides were UV-crosslinked at 150 mJ and store in a desiccator until use. Lucidea Universal ScoreCard (GE Healthcare) spike controls were diluted in 100 ng/μL spotting buffer and printed on the array for quality evaluation. Each calibration and negative controls from the Lucidea kit were spotted several times across the whole area of the array. Every selected clone was spotted once.
  Martinez-Godoy MA, Mauri N, Juárez J, Marques MC, Santiago J, Forment J, Gadea J (2008) A genome-wide 20k citrus microarray for gene expression analysis. BMC Genomics 9:318.