title: IMUL-Candida glabrata 8x15k
status: Public on Jul 24 2010
contact.city: Lausanne
contact.first: Dominique
contact.last: Sanglard
contact.email: Dominique.Sanglard@chuv.ch
contact.address: Rue Bugnon 48
contact.country: Switzerland
contact.web_link: http://www.chuv.ch/imul
contact.institute: University of Lausanne and University Hospital Center
organism: Nakaseomyces glabratus CBS 138
description: The nucleotide sequences of the 5283 C. glabrata ORFs and the mitochondrial genome (release 2) were downloaded from the Génolevure Consortium (http://www.genolevures.org/). Following the Agilent eArray Design guidelines, two separate probe sets for each ORF were designed, each consisting of 60 base oligonucleotides. The probe selection was performed using the GE Probe Design Tool; probes were filtered following their base composition and distribution, cross-hybridization potential and melting temperature to yield two probes sets representing each 5210 nuclear and 6 mitochondrial ORFs. These probes cover more than 98% of the nuclear genome and represent 6 out of the 8 mitochondrial protein-encoding genes. For quality control and normalization purposes, 103 probes were selected randomly and spotted 20 times throughout each array in addition to standard Agilent controls. C. glabrata custom arrays were manufactured in the 8x15k format by Agilent Technologies.
manufacture_protocol: see manufacturer's web site: http://www.agilent.com/