title: CRG Human Splicing Array Custom 44K G2514F AMADID19952
status: Public on Mar 07 2011
contact.city: barcelona
contact.first: BELEN
contact.last: MINANA
contact.email: belen.minana@crg.es
contact.state: barcelona
contact.address: dr aiguader 88
contact.department: GENE REGULATION, STEM CELLS AND CANCER
contact.country: Spain
contact.institute: Center for genomic regulation
contact.phone: 0034933160276
organism: Homo sapiens
description: It was designed an alternative splicing-sensitive microarrays which focus on the analysis of two groups of genes: splicing regulatory factors and cancer-related genes, with the goal of establishing correlations between changes in splicing factor expression and pre mRNA alternative splicing under different biological situations. Databases of human SReg and other proteins involved in RNA metabolism (420 genes) and of genes implicated in tumor onset and progression (500 genes) were generated. Splicing events (about 1000 for each category) were identified computationally by alignment of genomic sequences to RefSeq, mRNA and ESTs. Probes complementary to alternative and constitutive exons as well as to splice junctions were selected and produced using AGILENT ink-jet technology.
  Barcodes: from 251995210001 to 251995210099.
manufacture_protocol: see manufacturer's web site at http://www.agilent.com