title: Candida_albicans_ArrayIB
status: Public on Jun 15 2009
contact.city: Boston
contact.first: Anna
contact.last: Selmecki
contact.email: selme003@umn.edu
contact.state: MA
contact.address: 450 Brookline Ave
contact.country: USA
contact.institute: Dana-Farber Cancer Institute
organism: Candida albicans
description: The same probes were used for this platform and all the other platforms.  This is also the same probe layout as ArrayI, just a different printing date.
manufacture_protocol: Primer pairs were used to amplify Candida albicans ORFs from SC5314 genomic DNA. PCR fragments were purified on MultiScreen-PCR 96-well filtration units (Millipore, Bedford, Mass.) and were eluted in spotting buffer (3 SSC [(1 SSC is 0.15 M NaCl plus 0.015 M sodium citrate] plus 0.01% SDS). Purified PCR products were spotted in duplicate on poly-L-lysine-coated slides with a MicroGrid II (BioRobotics, Cambridge, United Kingdom). Slides were postprocessed with succinic anhydride in 1-methyl-2-pyrrolidinone (http://www.microarrays.org/protocols.html).