title: Mouse Immunology 384 StellARray™ qPCR Array plates (# 00188202)
status: Public on Mar 28 2011
contact.city: London
contact.first: Diane
contact.middle: Marylin
contact.last: Scott
contact.email: diane.scott@imperial.ac.uk
contact.address: Du Cane Road
contact.department: Immunobiology
contact.country: United Kingdom
contact.institute: Imperial College London
contact.phone: +44 20838382852
organism: Mus musculus
description: This GEO Platform record represents two different plates, one custom made by Bar Harbor labs and another commercially available. This is designated by the 'plate' column in the Platform table below.
manufacture_protocol: Primers are arranged in a 384 well StellARray™ qPCR Array plates as dries samples.
  10ul mastermix made from: Power Sybr green master mix, 1814.4μl DEPC treated water (Ambion) and 201.6μl cDNA diluted 1:10 with DEPC treated water, was added to each well.
  The plates were sealed using an optical adhesive seal
  The plates were briefly centrifuged and left at room temperature for 15min
  The plates were run on a 7900HT sequence detection system using 384 well block and standard cycling conditions (95ºC 10mins followed by 40 cycles of 95 ºC for 15sec and 60 ºC for 1 min).