title: Whole genome oligonucleotide microarray for Protochlamydia amoebophila
status: Public on Feb 04 2011
contact.city: Vienna
contact.first: Doris
contact.last: Steger
contact.state: Vienna
contact.address: Althanstrasse 14
contact.department: Department of Microbial Ecology
contact.country: Austria
contact.institute: University of Vienna
organism: Candidatus Protochlamydia amoebophila UWE25
manufacture_protocol: 5´-amino modified oligonucleotide probes were printed onto silylated amine-aldehyde glass slides using a BioRobotics MicroGrid 610/TAS system and 16 MicroSpot 2500 split pins. The average spot diameter was about 150 μm and spot centres were 300 μm apart. The relative humidity during spotting was set to 50% within the spotting compartment. Spotted microarrays were stored one day at 100% humidity. Subsequently, slides were washed by soaking them twice in 0.2% SDS for 2 min and twice in double-distilled water for 2 min. After air drying for 5 min, the slides were incubated in freshly prepared sodium borohydride solution. Then, slides were rinsed in ice-cold absolute ethanol and subsequently washed two times in 0.2% SDS and two times in double-distilled water. Finally, slides were dried by centrifugation.