title: Rattus norvegicus 5K
status: Public on Mar 15 2017
contact.city: Estado de México
contact.first: Ronquillo Sánchez
contact.middle: Sánchez
contact.last: María Dolores
contact.state: Tlalnepantla
contact.address: Av. De los Barrios No. 1, Los Reyes, Iztacala
contact.department: Unidad de Biomedicina, Facultad de Estudios Superiores Iztacala
contact.country: Mexico
contact.institute: Universidad Nacional Autónoma de México
organism: Rattus norvegicus
manufacture_protocol: Rattus norvegicus 70-mer oligo library from OPERON Oligo Sets (http://omad.operon.com/) It contains 5,000 gene specific oligonucleotide probes representing the better known genes, were resuspended to 40µM in Micro Spotting solution (Telechem International Inc.). SuperAmine coated slides 25x75 mm (TeleChem International INC) were printed in two copies, and fixed at 80°C for 4 hours. For pre-hybridization the slides were re-hydrated with water vapor at 60°C, and fixed with two cycles of UV light (1200J). After boiling for two minutes at 92°C, slides were washed with 95% ethanol for one minute and prehybridzed in 5X SSC, 0.1% SDS and 1% BSA for one hour at 42°C .The slides were washed and dried for further hybridization.