title: Zebrafish 34K (Compugen, MWG, Operon)
status: Public on Jul 20 2007
contact.city: Bethesda
contact.first: Benjamin
contact.last: Feldman
contact.email: bfeldman@mail.nih.gov
contact.state: MD
contact.address: 35 Convent Drive
contact.department: National Human Genome Research Institute
contact.country: USA
contact.institute: National Institutes of Health
organism: Danio rerio
description: The zebra fish 35k chip is a combination of 16,399 60 mer oligos from Compugen http://www.labonweb.com/oligo/libraries.htm
  14,067 50 mers from MWG. http://www.ocimumbio.com/web/arrays/oligo_zebrafish.asp
  and 3500 70 mer oligos from Operon. http://www.operon.com/arrays/oligosets_zebra.php
  To look for the genes in the Compugen/MWG original list, click here (http://magic2.nhgri.nih.gov/mcore/zebrafish_genelist.txt)
  General questions or comments about this specific platform please direct to:
  Abdel G. Elkahloun: abdel@mail.nih.gov (tel. 301-402-3170 )
  Settara Chandrasekharappa: chandra@mail.nih.gov (tel. 301-402-2344)
manufacture_protocol: Microarray preparation. The 33K Zebra fish oligo array consist of three oligo sets: Compugen (with 16,512 60mers), MWG (with 14,240 50mers) and Operon (3,479 70mers). The set includes 170 positive (known house keeping gene) and 244 negative control oligos (Random sequences) to control the homogeneity and specificity of the hybridization. The 5' amine modified oligos were resuspended in 3xSSC and printed on Epoxy slides from Corning Life Sciences.