title: Nanjing University 22K Human Genome Array
status: Public on May 06 2010
contact.city: Nanjing
contact.first: Yu
contact.last: Tang
contact.email: cattlemouse@sohu.com
contact.state: Jiangsu Province
contact.address: 321 Zhongshan Road
contact.department: Department of Rheumatology and  Immunology
contact.country: China
contact.institute: The Affiliated Drum Tower Hospital of Nanjing University Medical School
contact.phone: +86-25-8310-5219
organism: Homo sapiens
manufacture_protocol: 1.The labeled DNA was puriﬁed with a PCR PuriﬁcationKit (Qiagen), resuspended in Elution buffer and quantiﬁed.
  2. Labeled samples were quantitatively adjusted based on the efﬁciency of Cy-dye incorporation and mixed into 80μl hybridization solution (3×SSC, 0.2%SDS, 25%formamide, and 5×Denhart’s).
  3. DNA in hybridization solution was denatured at 95℃ for 3 min prior loading on a microarray.
  4. The array was hybridized at 42℃ overnight
  5. The array was washed with two consecutive washing solutions (0.2% SDS, 2×SSC at 42℃for 5min, and 0.2% SSC for 5 min at room temperature)