title: CIT M2 Mus musculus 1K BAC CGH array
status: Public on Sep 14 2006
contact.city: Paris
contact.first: Marc-Henri
contact.last: Stern
contact.email: tatiana.popova@curie.fr
contact.address: 26 rue d'Ulm
contact.department: Research Dept
contact.country: France
contact.institute: INSERM/Curie Institute
contact.phone: 33142346646
organism: Mus musculus
description: The construction of the mouse BAC-array was supported by grants from the Carte d Identite des Tumeurs (CIT) program of the Ligue Nationale Contre le Cancer.
manufacture_protocol: A home-made genome-wide mouse BAC array was built using 1081 BACs that were isolated, validated by end-sequencing and amplified by multiple displacement amplification (GE Healthcare). The 1017 validated clones, corresponding to 958 unique BACs, are considered for the analysis. The collection was spotted in quadruplicate (diameter 180 µm), in 75% formamide, on Corning Ultragaps aminosilane glass slides, using Telechem pins and a MicrogridII TAS printer (Biorobotics)