title: NCSU C. kronotskyensis 3K v1.0
status: Public on Jul 31 2015
contact.city: Raleigh
contact.first: Jeffrey
contact.last: Zurawski
contact.state: NC
contact.address: 840 Main Campus Drive Room 3309
contact.department: Chemical and Biomolecular Engineering
contact.country: USA
contact.institute: North Carolina State University
organism: Caldicellulosiruptor kronotskyensis
manufacture_protocol: A DNA microarray was designed and constructed based on 2583 protein-coding sequences in the C. kronotskyensis genome obtained from the Department of Energy's Joint Genomics Institute (https://img.jgi.doe.gov). OligoArray 2.1 (Rouillard, 2003) was used to generate one 30 to 60-mer oligonucleotide probe sequence for each open reading frame. The probes were synthesized (Integrated DNA Technologies, IA), re-suspended in 50% DMSO, and printed onto Ultragap microarray slides (Corning, NY) using a QArrayMini arrayer (Genetix, UK). Each probe was spotted five times onto each array to fortify statistical analysis.