title: Moldovan Lab Custom RT-PCR Array S/PC
status: Public on Jul 21 2014
contact.city: Indianapolis
contact.first: Michael
contact.middle: P.
contact.last: Murphy
contact.state: IN
contact.address: 1801 N Senate MPC2 #3500
contact.department: Surgery
contact.country: USA
contact.institute: Indiana University
organism: Homo sapiens
description: real-time PCR assay
manufacture_protocol: Primers (Qiagen) were diluted 1:20 with molecular-grade water, and 5 μL/well were added to 384-well plates using a Biomek® FX Laboratory Automation Workstation (Beckman Coulter, Inc., Brea, CA). The plates were left to dry overnight in a sterile hood and stored covered at -20ºC until use.