title: BCM Reverse Phase Protein Array RPPA0016
status: Public on May 29 2018
contact.city: st louis
contact.first: Cynthia
contact.last: Ma
contact.email: cynthiaxma@wustl.edu
contact.state: Missouri
contact.address: 4515 Mckinley, 4515 Mckinley
contact.department: Medical Oncology
contact.country: USA
contact.institute: Washington University
contact.phone: 3143628903
organism: Homo sapiens
description: While the tissue lysates were spotted on the slides, this platform includes the information on the antibodies used to assay these protein lysates.
manufacture_protocol: Protein lysates were prepared from PDX tumors with modified tissue protein extraction reagent (TPER; Pierce) and a cocktail of protease and phosphatase inhibitors (Roche Life Science). The lysates were diluted into 0.5 mg/mL of total protein in SDS sample buffer and denatured on the same day. The Aushon 2470 Arrayer (Aushon BioSystems) with a 40 pin (185 μm) configuration was used to spot samples and control lysates onto nitrocellulose-coated slides (Grace Bio-labs) using an array format of 960 lysates/slide (2880 spots/slide).   See also the PDF at the foot of this page.