title: mKIAA oligonucleotide microarray
status: Public on Apr 19 2005
contact.city: Kisarazu
contact.first: Kazuhide
contact.last: Imai
contact.email: imai@kazusa.or.jp
contact.state: Chiba
contact.address: 2-6-7 Kazusa-Kamatari
contact.country: Japan
contact.web_link: http://www.kazusa.or.jp/create
contact.institute: Chiba Industry Advancement Center
contact.phone: +81-438-52-3988
organism: Mus musculus
description: Description of KIAA and FLJ genes; Since 1994, a human cDNA project to accumulate information regarding the protein-coding sequences (CDSs) of unidentified human genes (at least the time they were sequenced) was initiated in Kazusa DNA Research Institute. They have focused their sequencing efforts on human long cDNAs encoded large proteins which might uncover unknown general aspects of the biological roles of these proteins.  As an outcome of this human cDNA sequencing project, we have reported the cDNA sequences of more than 2000 identified human genes (Nagase, T., et.al., 2001, DNA Res., 8, 319-327). The information regarding KIAA has been shared with the research community worldwide through the HUGE database (http://www.kazusa.or.jp/huge).
  Description of mKIAA and mFLJ genes; Since 2001, To relieve from legal and ethical restrictions on the use of human materials, we selected the mouse as a model organism to perform functional characterization of KIAA genes. For the first step, we began to isolate and characterize cDNA clones encoding mouse counterparts of human KIAA and FLJ proteins. We have already reported the cDNA sequences of approximately 1500 mouse KIAA-homologues (mKIAA genes) and 110 mouse FLJ-homologues (mFLJ genes) (Okazaki, N., et.al., 2004, DNA Res., 11, 205-218).The information regarding mKIAA and mFLJ genes has been shared with the research community worldwide through the ROUGE database (http://www.kazusa.or.jp/rouge/index.html).
  Description of mKIAA oligonucleotide microarray; "mKIAA oligonucleotide microarray" were spotted 511 long oligonucleotides on glass, consisting of 486 mKIAA genes, 3 mKIAA-paralogous genes, 1 mFLJ gene, 7 positive controls (housekeeping genes), and 14 negative controls (plant chloroplast genes: 1 spot of psaA, 1 spot of psbB, and 12 spots of rbcL). The oligonucleotides were designed according to four conditions: 1) lengths were between 65 and 75 bases; 2) melting temperatures were between 70 and 80°C; 3) GC contents were between 45 and 65%; and 4) representative unique sequences against 87,543 entries in mouse UniGene database (Build #113) were designed  by ProbeQuest software (Dynacom, Kanagawa, Japan).
  Keywords = Mouse
  Keywords = Long oligonucleotide
  Keywords = KIAA
  Keywords = mKIAA