title: Deinococcus Radiodurans R1_Microarray V1.0
status: Public on Feb 27 2010
contact.city: chongup
contact.first: sun
contact.middle: wook
contact.last: Jeong
contact.email: jeongsunwook@gmail.com
contact.state: Chonbuk
contact.address: Sinjeongdong 1266
contact.department: Radiation biotechnology
contact.country: South Korea
contact.institute: Korea atomic energy research institute
contact.phone: 82-063-570-3146
organism: Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539
manufacture_protocol: 1. PCR produts are arrayed in 96-well round-bottom plates. Each well contains 500ng/ul of PCR products in DW
  2. Mix with 5μL of PCR products in DW and 5μL of DMSO into 384-well, Genetix polystyrene V-bottom plates (cat# X6004)
  3. Seal plates with Corning seals.
  4. Incubate at 37℃ for 30 minutes to aid resuspension of DNA
  5. Shake plates near maximum rotational speed on flat-bed shaker for 1 minute.
  6. Centrifuge plates at 2000 rpm for 3minutes.
  7. Remove seals and cover with plate lids. Place  plates and slides(Corning GAPSII) in microarrayer
  8. Start printing slides according to microarrayer manual
  9. After the print run, put out 2 microarray slides from microarrayer and prescan them with microarray scanner for spot QC
  10. All microarray slide is preformed UV-cross linking to immobilize spotted DNA at 200mJ of UV energy
  11. Spotted cDNA microarrays are packed in slide case and deposited in dessicator