title: LinkGenomics Whole Genome DNA Microarray
status: Public on Mar 29 2013
contact.city: Houston
contact.first: Takahiro
contact.last: Kogawa
contact.email: tkogawa1@mdanderson.org
contact.state: TX
contact.address: 1515 Holcomb Blvd
contact.department: Breast Medical
contact.country: USA
contact.institute: MD Anderson
organism: Homo sapiens
manufacture_protocol: Whole Genome DNA Microarray was created with complete coverage of the human genome using 12310 individually amplified bacterial artificial chromosomes (BAC) clones. All BAC clones were cultured from a single colony and validated by PCR amplification using clone specific primers that were designed in-house. Extracted BAC DNAs were BstYI restricted and amplified by ligation-mediated PCR. The products were printed on glass slides with an inkjet-type spotter.