title: Carrington-Arabidopsis-Illumina-miRNA-AGO-high-throughput sequencing
status: Public on Aug 16 2008
contact.city: Saint Louis
contact.first: James
contact.middle: C
contact.last: Carrington
contact.email: jcarrington@danforthcenter.org
contact.state: MO
contact.address: 975 North Warson Road
contact.country: USA
contact.institute: Donald Danforth Plant Science Center
contact.phone: 314-587-1202
organism: Arabidopsis thaliana
description: Sequencing-by-synthesis technology was used to sequence small RNA from input and co-immunoprecipitation fractions from cell lysates of inflorescence  tissue from Arabidopsis plants transformed with HA epitope-tagged AGO2 or AGO7 constructs. Meaningful intepretation requires comparison between the input and co-immunoprecipitation datasets.
  Small RNA sequences were parsed from Illumina sequencing data files through identification of adaptor boundaries. Only sequences that mapped to the Arabidopsis thaliana Col-0 genome were included in the analysis.