title: RPPA-Homo spaiens
status: Public on May 12 2021
contact.city: Philadelphia
contact.first: lei
contact.last: guan
contact.email: guanlei32@gmail.com
contact.state: PA
contact.address: 433 S University Ave.
contact.department: Biology
contact.country: USA
contact.institute: university of pennsylvania
contact.phone: 2676945737
organism: Homo sapiens
manufacture_protocol: We stained slides for more than 400 unique antibodies and 3 secondary antibody negative controls which were analyzed on Array-Pro then by supercurve Rx64 3.1.1. There were 12 sets of replicated antibodies among slides. We performed QC test for each antibody staining (slide). The QC Score (Probability) values were also included in the data spreadsheet for your references. QC score above 0.8 indicates good antibody staining. We only included in the data for the all individual antibodies with QC Scores higher than 0.80 in the Heatmaps. In the case of antibodies with replicates, the one with the highest QC Score was used. For samples of tissue origin, we removed 7 antibodies cross react with damaged components (with unknown mechanism). There are 51 individual mouse antibodies (labeled -M) that will be removed for mouse tissue or xenograft samples, unless you specifically indicated for inclusion in your sample submission form.