title: M22K_01_UNAM-IFC_Murine_22K_oligoarray_version_v1
status: Public on Jul 04 2010
contact.city: Cuernavaca
contact.first: Sergio
contact.middle: Manuel
contact.last: Encarnación
contact.email: encarnac@ccg.unam.mx, esalazar@ccg.unam.mx
contact.state: Morelos
contact.address: Av. Universidad S/N
contact.department: Centro de Ciencias Genomicas
contact.country: Mexico
contact.institute: Universidad Nacional Autonoma de México
contact.phone: 777 3291899
organism: Mus musculus
description: The custom-designed M22K_01 oligonucleotide array contains a total of 44640 entries and was last updated at 2006. The microarray 65-mer probe sets represent 22,000 transcripts derived from approximately 70% genome annotated and predicted mouse genes and their alternative spliced variants (MGI/GenBank).
manufacture_protocol: Mus musculus twenty two thousand 65-mer oligo library sets from Sigma Genosys (http://www.sigmaaldrich.com/life-science/custom-oligos.html) were resuspended (50 μM) in Micro Spotting solution (ArrayIt Brand Products). SuperAmine coated slides 25 × 75 mm (TeleChem International, INC) were printed in duplicate and fixed at 80 °C for 4 h.