title: CCDTM Mm-CCDTM36k (derived from GPL5960)
status: Public on Oct 22 2011
contact.city: Doha
contact.first: Francesco
contact.middle: Maria
contact.last: Marincola
contact.email: fmarincola@sidra.org
contact.address: Al Nasr Tower, AL Corniche Street, PO Box 26999
contact.country: Qatar
contact.institute: Sidra Medical and Research Center
contact.phone: 301-793-8210
organism: Mus musculus
manufacture_protocol: The Operon mouse Array-Ready Oligo Set (AROS™) V 4.0 contains 35,852 long 70mer probes representing 25,000 genes and about 38,000 gene transcripts and also includes 380 controls. The oligo design is based on the Ensembl Mouse Database release 26.33b.1, Mouse Genome Sequencing Project, NCBI RefSeq, Riken full-length cDNA clone sequence, and other GenBank sequence (http://www.operon.com/). The oligonucleotide probes were purchased in 384-well polypropylene plates (Genetix, 384 micro array) and resuspend in 3XSSC print buffer at a concentration of 25uM. The microarray is composed of 48 blocks and one spot is printed per probe per slide. The spot size is 100 micron and spot distance is 157.5 microns (center-to-center). The microarrays were printed on SuperChip™ Epoxysilane MicroArray Slides (C50-5588-M20, Thermo Fisher Scientific Co) post-processed according to manufacture protocol.