title: PNL mixed species lignin-degrading enzymes microarray
status: Public on Apr 01 2009
contact.city: Richland
contact.first: Katrina
contact.middle: M
contact.last: Waters
contact.email: katrina.waters@pnl.gov
contact.state: WA
contact.address: 902 Battelle Blvd; MSIN J4-18
contact.department: Biological Sciences Division
contact.country: USA
contact.institute: Pacific Northwest National Laboratory
organism: Cryphonectria parasitica
  Neurospora crassa
  Phlebia radiata
  Pleurotus ostreatus
  Pleurotus eryngii
  Trametes versicolor
  Schizophyllum commune
  Agaricus bisporus
  Lentinula edodes
  Fusarium oxysporum
  Trametes cinnabarina
  Heterobasidion annosum
  synthetic construct
  Botrytis cinerea
  Gelatoporia subvermispora
  Coriolopsis gallica
  Dichomitus squalens
  Trametes coccinea
  Fusarium proliferatum
  Phanerodontia chrysosporium
manufacture_protocol: Probes were printed at 100 µM (in 100 mM Na2HPO4, 200 mM NaCl, 0.01% SDS print buffer) concentrations on epoxysilane-coated glass slides by a Microgrid II printing system (PerkinElmer Life And Analytical Sciences, Inc., Waltham, MA).  Control spots were present as buffer, beacon, biotin, polyA and polyT oligonucleotides.  The buffer spots were spots of blank print buffer (defined below) intended to ensure probe spots were well-spaced.  The beacon spot was a spot of Cy3 dye (always fluorescent), used to identify the orientation of the slide.  The biotin spot was included to check that biotin reporting was not hindered by the conditions used during hybridization or washing.  PolyA and polyT probes were included as positive and negative controls for the polyA-modified eukaryotic genes that are then reverse-transcribed to generate polyT tagged cDNA.