title: Invitrogen ProtoArray ProtoArray
status: Public on Feb 26 2018
contact.city: Boston
contact.first: Suzanne
contact.last: Walker
contact.state: MA
contact.address: 4 Blackfan Circle
contact.department: Microbiology and Immunobiology
contact.country: USA
contact.institute: Harvard Medical School
organism: Homo sapiens
description: Arrays from 3 different array batches are used in this study as described above in the description column of samples. Batches HA20358 (barcodes 74576-74579, 74581-74586, 74600, and 74617-74619), and HA20446 (barcodes 91384, 91412, and 91454) have identical test protein content arranged differently on the arrays, while HA20301 (barcodes 52630, 52641) has some differences in content. Content information is available on the basis of barcode on Invitrogen's website, and current batches of microarrays are available under catalog number PAH0525101
manufacture_protocol: Human proteins produced in insect cells are spotted in duplicate on a nitrocellulose glass slides in a 4x12 grid of subblocks, each of which includes several controls and test proteins in a 22x22 spot grid