title: Operon Human V2; print layout 1
status: Public on Jul 03 2007
contact.city: Boston
contact.first: Geoffrey
contact.middle: M
contact.last: Cooper
contact.state: MA
contact.address: 5 Cummington St
contact.department: Biology
contact.country: USA
contact.institute: Boston University
organism: Homo sapiens
description: Custom spotted oligonucleotide print of Operon's Human Array-Ready Oligo Set Version 2, layout 1.
manufacture_protocol: Microarrays were fabricated by resuspending 21,329 70-mer oligonucleotides from OperonÕs Human Genome Array-Ready Oligo Set Version 2.0 in 3X SSC to a final 30 micromolar concentration, and spotted onto amino-silane coated GAPS II slides (Corning) with an OmniGrid Accent microarrayer (GeneMachines).  After drying, the slides were post-processed according to the oligonucleotide manufacturerÕs protocol.  Briefly, to promote spot uniformity, the microarrays were rehydrated with nuclease-free water and snap-dried on a 100 degree C hot plate.  The slides were UV crosslinked with 65 mJ of energy and shaken for 20 min in a blocking solution of 1-methyl-2-pyrrolidinone, 171 mM succinic anhydride, and 43 mM sodium borate.  Finally, the slides were washed successively in water and 95% ethanol, then centrifuged at 800 rpm for 5 min to dry.