title: MIT/SharpLab Mouse ES cell 454 2007
status: Public on Oct 31 2007
contact.city: Cambridge
contact.first: J. Mauro
contact.last: Calabrese
contact.email: mauroc@mit.edu
contact.state: MA
contact.address: 40 Ames Street
contact.department: Biology
contact.country: USA
contact.institute: MIT
contact.phone: 617 253 6457
organism: Mus musculus
description: High throughput sequencing of mouse embryonic stem cell short RNAs, 16-30nt fraction.  Libraries were made from J1 ES cells before and 5 days after demethylation with 5-aza-deoxycytidine, and from Dicer-conditional ES cell lines before and after deletion of Dicer's floxed region.  Adaptor ligation required 5' monophosphates and 3' hyrdoxyls.