title: ACGT Pearl Millet SSH library 4K v1.0
status: Public on Jan 01 2009
contact.city: Pretoria
contact.first: Bridget 
contact.middle: Genevieve
contact.last: Crampton
contact.email: bcampbel@csir.co.za
contact.address: PO Box 395
contact.country: South Africa
contact.institute: CSIR Biosciences
contact.phone: +27128412535
organism: Cenchrus americanus
manufacture_protocol: 1. PCR fragments of a cloned cDNA suppression subtractive hybridisation (SSH) library are arrayed in Genetix X7022 384-well plates.
  2. Each well contains a final concentration of 125 ng/µl PCR product in 50% DMSO spotting solution (v/v).
  3. Between printing runs the plates are stored at -20°C.
  4. Prior to printing the plates are incubated at room temperature for 30 min to aid temperature equilibration.
  5. Plates are centrifuged at 2000 x g for 5 minutes prior to printing.
  6. Printing is performed on the Molecular Dynamics Generation III spotter.
  7. Seals are removed and plates placed in the spotter immediately prior to initiating the printing process to prevent unnecessary evaporation.
  8. A relative humidity of 50% is maintained in the spotter cabinet during printing runs.
  9. Following completion of the printing run the plates are sealed with a new Abgene adhesive plate seal and stored at -20°C.