title: Indiana University Onthophagus taurus 3.5K cDNA microarray version 1
status: Public on Jun 01 2009
contact.city: Bloomington
contact.first: Justen
contact.last: Andrews
contact.email: jandrew@bio.indiana.edu
contact.state: IN
contact.address: 915 East Third Street
contact.department: Biology
contact.country: USA
contact.web_link: http://www.bio.indiana.edu/~andrewslab/index.html
contact.institute: Indiana University
contact.phone: 812-855-6621
organism: Onthophagus taurus
manufacture_protocol: We mainly followed the protocol of Indiana University Drosophila Genomics Resource Center (Andrews et al. 2006, http://cgb.indiana.edu/publications/alias/dgrc1_prod_protocol/version/current) to print microarrays with a minor revision of post-print wash.  Cleaned clones were dried completely, re-dissolved with DGRC spotting solution (1.5M Betaine in 3xSSC), and spotted on GAPSII MIcroarray Slides (Corning).  By using OmniGrid 300 printing robot, we printed 100 microarrays.  Followed by heating the spotted microarrays at 85°C for 3hrs, we rinsed them with 5xSSC/0.1%SDS (55°C), water (room temperature) twice, hot water (95°C), and another room temperature water then centrifuged them to dry.