title: Utz Lab Whole Protein Autoantigen Array V2.0
status: Public on Jan 26 2012
contact.city: Stanford
contact.first: Chih Long
contact.last: Liu
contact.state: CA
contact.address: 269 Campus Drive
contact.department: Medicine - Imm. & Rheum
contact.country: USA
contact.institute: Stanford University
organism: Escherichia coli
  Gallus gallus
  Homo sapiens
  Bos taurus
  Oryctolagus cuniculus
  Mus musculus
manufacture_protocol: Whole protein or synthetic peptide antigens were purchased from commercial vendors as described in the data table.  Antigens were resuspended in 0.2 mg/ml in 1X phosphate-buffered saline (PBS) pH 7.4.  Custom microarrays were printed with the Bio-Rad Chip Writer Pro on Whatman FAST slides, with each antigen printed in 6 replicates.  Arrays were stored vacuum-packed for up to 2 months at 4 degrees Celsius before use.