title: State Key Lab_Human_13K_cDNA array
status: Public on Aug 01 2009
contact.city: Bristol
contact.first: Hai
contact.last: Fang
contact.email: hfang@cs.bris.ac.uk
contact.address: Woodland Road
contact.department: Department of Computer Science
contact.country: United Kingdom
contact.institute: University of Bristol
organism: Homo sapiens
manufacture_protocol: Microarrays with 12,630 cDNA clones representing 10,647 genes were fabricated in-house using a Generation III spotter (Amersham Biosciences). The cDNA clones were sequence verified and enriched with genes expressed in hematopoietic cells (Mao et al. 1998; Zhang et al. 2000). Among these cDNA clones include commercial clones from Invitrogen. The Scorecard plate including positive control, negative control, dynamic range control, ratio control and housekeeping genes were spotted on each slide. Microarray slides were obtained from Fullmoon BioSystems (Sunnyvale, CA, USA). The clones were spotted in a final concentration of 200-400 fmol/µl in spotting buffer (50% DMSO) using 12 microspot pins to reach a complexity of 12,630 spots per slide. After spotting, the slides were UV cross-linked (400 mJoules) and stored at room temperature.