title: NEFU Brasicca rapa 3k v1.0
status: Public on Feb 22 2007
contact.city: Bunkyo-ku
contact.first: Saneyuki
contact.last: Kawabata
contact.email: ayuki@mail.ecc.u-tokyo.ac.jp
contact.state: Tokyo
contact.address: 1-1-1 Yayoi
contact.country: Japan
contact.institute: University of Tokyo
organism: Brassica rapa subsp. rapa
manufacture_protocol: A subtractive cDNAs libraries were constructed from Brassica rapa by conducting suppression subtractive hybridization (SSH) between sunlight exposed and shaded hypocotyls. Randamly selected 4.2k clones were sequenced and clutered by 'blastclust'. Basically, one clone for each cluster, 2.8k clones in total, was chosen as probes. The amplified inserts of these clones were printed in duplicate by Kaken Genecs (Chiba, Japan).