title: BMFZ Mouse 40K V.1 part 2
status: Public on May 31 2010
contact.city: Duesseldorf
contact.first: Antje
contact.last: Lindecke
contact.email: antje.lindecke@uni-duesseldorf.de
contact.address: Universitaetsstr. 1
contact.department: BMFZ
contact.country: Germany
contact.institute: Universitaet Duesseldorf
contact.phone: 49-211-8114367
organism: Mus musculus
description: spotter = QArray (Genetix)
manufacture_protocol: 1. Oligos are purchased from Operon (AROS Mus Musculus V.4.0, plate 49-95 + control plate and buffer plate) arrayed in Genetix V-bottom 384-well plates. Each well contains 300 pmol of 70-mer oligo.
  2. A day before printing oligos were resuspend in 15 µl Amino-Spotting-Buffer ((Genetix Ltd., Hampshire, UK)
  3. Centrifuge plates at 1000 rpm for 5 minutes.
  4. Plates were sealed and store at 8°C over night
  5. Centrifuge plates at 1000 rpm for 5 minutes.
  6. Remove seals and cover with plate lids. Place in appropriate location of plate cassette.
  7. After the print run is completed, allow plates to dry through passive evaporation in a protected environment.
  8. For each subsequent preparation of these plates for a print run, add water to the wells instead of spotting buffer. The amount of water should be decreased by 0.5 µL per print run, as this is the amount drawn up by the pin capillary during each print run.