title: TIGR_HUMAN_40k_cDNAarray_1
status: Public on Jun 21 2008
contact.city: Washington, DC
contact.first: Norman
contact.middle: H
contact.last: Lee
contact.email: phmnhl@gwumc.edu
contact.state: DC
contact.address: 2300  I Street, N.W.
contact.department: Pharmacology & Physiology
contact.country: USA
contact.institute: The George Washington University Medical Center
organism: Homo sapiens
description: The arrays are printed on the barcoded side of the slide with approximate distance from the top, left side and right side of 3 mm. The array consists of 48 grids with each grid representing 832 spots in a 26 column x 32 row arrangement.
manufacture_protocol: The TIGR_HUMAN_40k_cDNAarray_1 consists of 39,936 elements from the Research Genetics 40K sequence-verified clone human cDNA library. Purified cDNA amplicons were combined with printing buffer to a final buffer concentration of 50% DMSO. The 384-well printing plates were arrayed onto aminosilane glass slides (Corning UltraGAPS) on a custom -built microarray robot from Brooks Automation (Cambridge, MA). Printed arrays were UV cross-linked to ensure binding.