title: Human-mouse microRNA array v8.0
status: Public on Mar 31 2005
contact.city: Chapel Hill
contact.first: Scott
contact.middle: M
contact.last: Hammond
contact.email: hammond@med.unc.edu
contact.state: NC
contact.department: Dept Cell and Developmental Biology
contact.country: USA
contact.institute: University of North Carolina
contact.phone: 919-843-2366
organism: Homo sapiens
  Mus musculus
description: Oligonucleotide probes to 191 human microRNA genes and 198 mouse microRNA genes were designed, antisense to the mature sequence as reported in the Sanger microRNA registry (4/1/04). Probes were spotted at 40 uM on Corning GAPS-II slides in 3X SSC, dried, and crosslinked by 600mJ UV. Slides were prehybridized in 3x SSC, 0.1% SDS, 0.2% BSA for 45 minutes, hybridized for 2h at 37C in 400mM Na2HPO4 pH 7.0, 0.8% BSA,
  5% SDS, 12% formamide.