title: AgResearch, Rumen Microbial Genomics_Bpr_Mru_20K_v1_Print 121
status: Public on Jan 28 2010
contact.city: Urbana
contact.first: Carl
contact.middle: James
contact.last: Yeoman
contact.email: cjyeoman@illinois.edu
contact.state: IL
contact.address: 1207 W Gregory ave
contact.department: Institute for Genomic Biology
contact.country: USA
contact.institute: University of Illinois
contact.phone: 217 7663382
organism: Butyrivibrio proteoclasticus B316
  Methanobrevibacter ruminantium M1
manufacture_protocol: 70mer and 50mer oligonucleotide probes were designed to the ORFs identified by GLIMMER in the draft sequences of B. proteoclasticus and M. ruminantium using ROSO (Recherche et Optimisation de Sondes Oligonucleotidiques; Reymond et al. 2004; Available: [http://pbil.univ-lyon1.fr/roso]. Controls included Blanks, 8 Arabidopsis thaliana genes and randomly in-silico mutated genes from B. proteoclasticus. 70mer oligonucleotides meeting the design parameters of ROSO were
  synthesized by Illumina (San Diego, CA, USA) and subsequently spotted in triplicate onto the surface of an epoxy coated slide (Corning, Lowell,
  MA, USA) using an ESI robot (Engineer Service Inc., Toronto, Ontario, Canada). In addition to the 70mers, triplicate 50mers were also printed for genes deemed to be of most importance to this research program. Features were printed with 100 um diameter.  Hybridisation-ready microarrays were stored desiccated at room temperature until use.