title: DualChip human SBCL
status: Public on Mar 25 2009
contact.city: Namur
contact.first: jean-pierre
contact.last: gillet
contact.email: jean-pierre.gillet@unamur.be
contact.address: 61 Rue de Bruxelles
contact.department: URPhyM
contact.country: Belgium
contact.institute: University of Namur
contact.phone: 003281724275
organism: Homo sapiens
manufacture_protocol: The genes on the low density microarray (termed DualChip human SBCL) are presented in Table I in Gillet JP et al. J. Pathol. in submission. This microarray contains two arrays per slide with a range of 107 genes whose role in classification or lymphomagenesis of small B-cell lymphomas is known. The microarray is based on the Xmer technology developed by Eppendorf (Hamburg, Germany). It is composed of single strand DNA probes attached to a glass support by a covalent link. Each DNA probe is present in triplicate on the array and designed for the detection of gene expression (Figure 1).
  To minimize the effect of variation sources, the microarray contains several controls which are also intended for normalization of signal intensities. This set of controls includes positive and negative hybridization controls, a positive detection concentration curve, negative detection controls, internal standards (mix of spiking RNAs), and a set of housekeeping genes. The specificity of the capture-probes has been checked in vitro and in silico. The reproducibility and the sensitivity of low density arrays (termed DualChip) were demonstrated previously:
  Gillet JP, Efferth T, Steinbach D, Hamels J, de Longueville F, Bertholet V et al. Microarray-based detection of multidrug resistance in human tumor cells by expression profiling of ATP-binding cassette transporter genes. Cancer Res 2004; 64:8987-8993.
  de Longueville F, Lacroix M, Barbuto AM, Bertholet V, Gallo D, Larsimont D et al. Molecular characterization of breast cancer cell lines by a low-density microarray. Int J Oncol 2005; 27:881-892.
  Meneses-Lorente G, de Longueville F, Dos Santos-Mendes S, Bonnert TP, Jack A, Evrard S et al. An evaluation of a low-density DNA microarray using cytochrome P450 inducers. Chem Res Toxicol 2003; 16:1070-1077.