title: Citrus 19K cDNA
status: Public on Mar 10 2009
contact.city: Wuhan
contact.first: Qing
contact.last: Liu
contact.email: liuqing08@webmail.hzau.edu.cn
contact.address: Shizishan street NO.1
contact.department: National Key Laboratory of Crop Genetic Improvement
contact.country: China
contact.institute: Huazhong Agricultural University
organism: Citrus sinensis
manufacture_protocol: The amplified cDNA PCR products were precipitated with the addition of 100 μl of anhydrous ethanol and resuspended in 15 μl of 50% dimethyl sulphoxide (DMSO) at a final concentration of 0.1-0.5 μg/μl and then spotted onto amino silaned glass slides (CapitalBio.Corp, Beijing, China) with a SmartArrayerTM microarrayer (CapitalBio Corp.). Each clone was printed in triplicate. After printing the slides were baked for 1 h at 80 °C and stored dry at room temperature