title: Agilent-015037 Bulyk Human Muscle CRMs 244K v1.0
status: Public on Feb 01 2012
contact.city: Boston
contact.first: Martha
contact.middle: L
contact.last: Bulyk
contact.email: mlbulyk@receptor.med.harvard.edu
contact.state: MA
contact.address: 77 Avenue Louis Pasteur
contact.department: Division of Genetics
contact.country: USA
contact.institute: Brigham and Women's Hospital
contact.phone: 617-525-4725
organism: Homo sapiens
description: The array probes were designed to achieve 25-bp resolution tiling of 100 kb surrounding each of 104 selected human genes, chosen because they were either upregulated over the timecourse of myogenic differentiation, or annotated by Gene Ontology as being a part of the muscle sarcomere. For each of these genes, the array covers -40 kb to +10 kb relative to transcription start and -10 kb to +40 kb relative to transcription stop. Additionally, the array sequence covers: 5 kb around each of 9 negative control regions previously found to be not bound by myogenic TFs; 5 kb around each of 35 potentially regulatory noncoding SNPs, and 5 kb around each of 100 predicted CRMs which are distant (i.e., greater than 50 kb away from the TSS) from annotated muscle-specific or differentially expressed genes. The probe sequences were selected using the microarray design tools available from Gerstein and colleagues (http://tiling.gersteinlab.org/microarray_design.html) to mask repetitive sequences.
  catalog number: AMADID # 015037
manufacture_protocol: Standard Agilent commercial synthesis protocols