title: Clostridium difficile 630/QCD32g58 array
status: Public on Feb 01 2008
contact.city: Ithaca
contact.first: Tavan
contact.last: Janvilisri
contact.email: tj57@cornell.edu
contact.state: NY
contact.address: Veterinary Research Tower, College of Veterinary Medicine, Cornell University
contact.country: USA
contact.institute: Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University
organism: Clostridioides difficile
description: The genome sequences of C. difficile 630 and QCD-32g58 were used as a source of the genes used in this study. The final arrays consisted of 13,824 spots corresponding to 4227 coding sequences present in both or either strains, with many genes represented by multiple spots. Genes that lacked unique regions were excluded (126 genes from the strain 630 and 34 genes from the strain QCD-32g58).
manufacture_protocol: Oligonucleotides of 55 to 70 bp with a matched melting temperature of ~60 C were designed to be specific for each gene. The probes (70 mer) were synthesized (Operon) and suspended in 50% dimethyl sulfoxide and spotted as triplicates onto Corning UltraGAPS slides using a custom-built microarrayer and Telechem 946 pins at the Cornell University Microarray Core Facility (www.bigredspots.cornell.edu). Autoblank (buffer without oligos) was used as a negative control. The slides were processed according to the slide manufacturer’s instructions, using hydration and UV irradiation, and stored in a dark, dust-free environment.