title: E coli O157 array virulence SOS
status: Public on Apr 03 2007
contact.city: Lubbock
contact.first: Scot
contact.last: Dowd
contact.email: sdowd@pathogenresearch.org
contact.state: TX
contact.address: 4321 Marsha Sharp Fwy
contact.department: MBRI
contact.country: USA
contact.web_link: http://www.medicalbiofilm.org
contact.institute: MBRI
contact.phone: 806-789-6879
organism: Escherichia coli
description: epoxide
manufacture_protocol: Using the transcriptome of E. coli O157:H7 EDL933 an oligonucleotide microarray, made up of 43-45 mers, was designed.The genes on the O157 array were selected based upon their being associated with virulence or with regulation of virulence genes.  Included in this new version are genes associated with pO157, SOS response, and BP933W. Specifications of oligos were based upon various design characteristics such as temperature of melting, 3’ location, specificity, lack of repeat nucleotides, etc. [32]. Oligos were synthesized and normalized in concentration by Integrated DNA Technologies Inc. (Coralville, IA). Oligos were resuspended in Epoxide Slide Spotting Solution and printed onto Epoxide Coated Slides (Corning Inc., Corning, NY) using a GeneMachine’s Omnigrid Accent microarray printer (Genomic Solutions Ann Arbor, MI).