title: CapitalBio Human oligo chip 35k array V2.0
status: Public on Jun 10 2012
contact.city: Beijing
contact.first: cheng
contact.last: long
contact.address: 27 Tai-Ping Lu Rd
contact.country: China
contact.institute: Beijing Institute of Biotechnology
organism: Homo sapiens
description: CapitalBio Human oligo chip 35k V2.0 containing 35,000 human gene elements
manufacture_protocol: 1.The oligo library (Operon Human Genome Oligo Set Version 4.0 library) and additional internal and external controls are spotted on a 75×25 mm chemically modified slide using a SmartArrayer™ microarray spotter (CapitalBio Corp.)
  2.Hydrate the slide over a 60℃ water bath for 10 seconds by holding the microarray slide label-side down about 2-3 cm above the water vapor. Then allow the microarray to dry at room temperature for 10 min and then hydrate again for another 10 seconds. Watch spots carefully to avoid over-hydration (where the spots begin to merge together).
  3.Cross-link the slide by exposing the slides label-side up to 250 mJ/cm2 UV light in a commercial UV cross-linker.
  4. Wash in 0.5% SDS preheated to 42℃ on a horizontal shaker (80 rpm) for 10 min.
  5. Wash in distilled water preheated to 42℃ on a horizontal shaker (80 rpm) for 4-5 min.
  6. Wash in ethanol on a horizontal shaker (80 rpm) for 2-3 min.
  7.Transfer the slide to a conical 50 ml centrifuge tube and centrifuge for 1 min at 2,000 rpm to remove the surface liquid. Then the microarray is ready for hybridization.