title: Helmholtz Centre Streptococcus mutans UA159 6k array (uncondensed)
status: Public on Jul 26 2006
contact.city: Braunschweig
contact.first: Robert
contact.last: Geffers
contact.email: robert.geffers@helmholtz-hzi.de
contact.address: Inhoffenstr. 7
contact.department: Dep. Molecular Bacteriology
contact.country: Germany
contact.institute: HCI - Helmholtz Centre for Infection Research
contact.phone: +49 531-6181-3058
organism: Streptococcus mutans UA159
description: A condensed version of this array is represented in Platform GPL9332.
manufacture_protocol: 1957 oligonucleotides were deposited onto CodeLink activated slides (Amersham Biosciences) at a concentration of 25 µM in 1.5 x sodium phosphate buffer in a contact-dependent manner using a MicroGrid TAS II spotter (BioRobotics, Freiburg, Germany). All 50-mers were amino-modified at the 5’-end enabling covalent linkage to reactive ester groups provided by the glass surface. Coupling of DNA was ensured by overnight incubation in a saturated sodium chloride chamber and blocking residual reactive groups was done as recommended by the manufacturer (Amersham biosciences). Until they were used, slides were maintained in a desiccated environment. To ensure complete spotting, SYBR-Green staining of three randomly selected microarrays of each printing batch was performed.
  The S. mutans UA159 chip consists of 1952 oligonucleotides probing genes specific for ORFs at genomic scale and five Arabidopsis thaliana sequences served as hybridization controls and were used for grid finding. Altogether, three replicates were immobilized per oligonucleotide and array. Two arrays were placed on one slide.