title: Human 27.7K oligo probe set v 3.0
status: Public on Mar 05 2009
contact.city: Silver Spring
contact.first: Rasha 
contact.last: Hammamieh
contact.email: rasha.hammamieh1@us.army.mil
contact.state: MD
contact.address: 503 Robert Grant Ave
contact.department: Molecular Pathology
contact.country: USA
contact.institute: Walter Reed Army Institute of Research
contact.phone: 301-319-9056
organism: Homo sapiens
manufacture_protocol: The oligonucleotide targets were deposited in 3X saline sodium citrate (SSC) at an average concentration of 165 μg/ml on CMT-GAPS II aminopropyl silane-coated slides (Corning, Corning, NY) using a VersArray microarryer (Bio-Rad, Inc). Arrays were post processed by UV-cross linking at 1200 µJoules/cm2  100 using UV Stratalinker 2400 (Stratagene Inc., La Jolla, CA). Then slides were baked for 4 hours at 800C, and the positively charged amine groups on the slide surface were treated with succinic anhydride/N-methyl-2-pyrrolidinone.