title: Human BAC to study Chromosome 21 abnormalities
status: Public on Feb 26 2004
contact.city: Columbus
contact.first: Herbert
contact.last: Auer
contact.email: auer-2@medctr.osu.edu
contact.state: OH
contact.address: 420 West 12th Ave
contact.country: USA
contact.web_link: http://www.dnaarrays.org
contact.institute: Human Cancer Genetics
contact.phone: 614 247 7116
organism: Homo sapiens
description: 36 RPCI-11 BAC clones covering the q-arm of chromosome21 from position15.1MB(close to the centromere)to the telomeric position 46.9 MB with an average gap between clones of 800 kb (range 346-1593 kb)	In addition 23 randomly selected clones, each representing 1 of the remaining chromosomes1-20,22,X and Y"Positions of genes and BAC clones were determined according to the NCBI (http://www.ncbi.nlm.nih.gov/mapview) 59 BAC clones spotted in 6 replicas for a total 354 spots on GAPII corning glass slides using Affymetrix GMS417 Affymetrix Arrayer with 4-ring&pin cofiguration. (Affymetrix,Santa Clara, CA). Purified clones spotted in 50%DMSO" DNA Isolation of these clones was performed from bacterial culture (250ml) using Qiagen midi kit (Qiagen, Valencia CA).
  Keywords = Chromosome 21 copy number alterations