title: HGMP NIA 15 K, slide 21-40 (2 of 2). Printed by HGMP, Hinxton
status: Public on Jul 11 2007
contact.city: Cardiff
contact.first: Fiona
contact.middle: Catherine
contact.last: Mansergh
contact.email: mansergf@tcd.ie
contact.state: Wales
contact.address: Museum Avenue
contact.department: Biosciences
contact.country: United Kingdom
contact.institute: Cardiff University
contact.phone: 0035318962484
organism: Mus musculus
description: Second slide of 2. HGMP printed NIA 15K set, over 2 slides, 17280 spots per slide, including empties, control spots, landmark spots, and duplicate experimental spots. 12 rows and 4 columns of grids, each grid contains 18 rows and 20 columns of spots (format for both slides 1-20 and 21-40).
manufacture_protocol: cDNA arrays (now discontinued), spotted PCR product. Bacterial source material diluted 1/10 prior to PCR,
  primers: Ko forward primer (amidated): GTG TGG AAT TGT GAG CGG ATA ACA A (25mer) with 5' NH2(C6) modification.
  Ko reverse primer: CCA GTC ACG ACG TTG TAA AAC GAC (24mer). Purified using Millipore Multiscreen PCR plates. Purified PCR products have then been rearrayed into 384 well plates and printed. 20µl of spotting buffer  is added to 10µl of PCR product (0.1ug/ul) and this is sufficient to grid over 1000 glass arrays.
  The manufacturers ask that the following paper describing the clones is cited in connection with these arrays: Tanaka TS, et al Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray. PNAS (2000) 97: 9127-32