title: Utz Lab human high-throughput immunophenotyping using transcription (HIT) array
status: Public on Mar 03 2008
contact.city: Stanford
contact.first: Michael
contact.middle: George
contact.last: Kattah
contact.email: mkattah@stanford.edu
contact.state: CA
contact.address: 269 Campus Dr., CCSR 2250
contact.department: Medicine
contact.country: USA
contact.institute: Stanford University
contact.phone: 650-804-1252
organism: Homo sapiens
description: This method employs a panel of monoclonal antibodies, each tagged with a unique oligonucleotide sequence that serves as a molecular barcode.  After staining a sample, T7 polymerase amplifies the tags which are then hybridized to a DNA microarray (see data table below) for indirect measurement of each analyte.
  The Platform data table reflects a condensed representation of the array's replicate features.  The full array layout representing all of the individual features is linked as a supplementary file at the foot of this record.
manufacture_protocol: For the microarrays, we spotted amine-modified 30mer oligonucleotides onto NHS-ester coated Codelink slides (GE Healthcare) at a concentration of 50 μM in 50 mM sodium phosphate buffer pH 8.5 (Sigma-Aldrich) using a BioRad VersArray Compact Microarrayer (BioRad) and processed the slides according to the manufacturer’s instructions.