title: IFTC Saccharomyces cerevisiae 154 v1.0
status: Public on Dec 12 2008
contact.city: Leipzig
contact.first: Franziska
contact.last: Heine
contact.email: franziska.heine@ufz.de
contact.address: Permoserstraße 15
contact.department: Environmental Microbiology
contact.country: Germany
contact.institute: Helmholtz-Centre for Environmental Research - UFZ
organism: Saccharomyces cerevisiae
description: The microarray presents 154 oligonucleotides (70mer) in triplicate encoding for selected proteins of the G1- and S-phase from the cell cycle of S. cerevisiae as well as selected oligonucleotides encoding for proteins belonging to the various metabolic pathways, glucose transporters and flocculins of S. cerevisiae.
manufacture_protocol: Oligonucleotide design and synthesis: Oligonucleotides were designed and synthesised by the manufacture Operon excepting oligonucleotides representing Lg-FLO1. These oligonucleotides were designed using DNA-Star, blasted at NCBI (National Center for Biotechnology Information) and afterwards synthesised by Operon.
  Spotting protocol: To produce microarrays silylated slides were purchased from TeleChemTM International Inc. (Sunnyvale, CA, USA) and the printing of the oligonucleotides was performed by using an AffymetrixTM 417 arrayer. Oligonucleotide solutions were prepared in 96-well-plates containing each 30 µl. The concentration of the printed oligonucleotides was 50 µM in 1x ArrayItTM Microarray Spotting Solution Plus. After spotting, the slides were incubated at 80 °C for 2 h. Slides were blocked in SSC containing 1% BSA (Sigma) for 45 min and rinsed with water for 5 min.