title: Combimatrix Orientia tsutsugamushi customArray 4x2k
status: Public on Mar 31 2010
contact.city: Seoul
contact.first: Nam-Hyuk
contact.last: Cho
contact.email: chonh@snu.ac.kr
contact.address: 28 Yongon-Dong, Chongno-Gu
contact.department: Microbiology and Immunology
contact.country: South Korea
contact.institute: Seoul National Univ. College of Medicine
contact.phone: +82-2-740-8392
organism: Orientia tsutsugamushi
description: CustomArray used a specially modified “CMOS” semiconductor to synthesized oligonucleotide probe of a specific sequence of DNA bases in response to a digital command. 27 to 40-mer oligonucleotide probes (1613 probes) were designed for 1472 CDSs of the O. tsutsugamushi genome (GeneBank accession no. AM494475). 1 to 6 probes per CDS were designed and some of the probes were duplicated on the microarray (total 2187 spots on an array). As negative controls, 5 and 15 oligonucleotide probes derived from plant and bacteriophage respectively were spotted on the array at 53 sites per array.
manufacture_protocol: http://www.combimatrix.com/support_faq4X2.htm