title: Splitter_Bmelitensis_289mini_v1
status: Public on May 01 2008
contact.city: Madison
contact.first: Gary
contact.last: Splitter
contact.email: splitter@svm.vetmed.wisc.edu, angelajmathison@uwalumni.com
contact.state: WI
contact.address: 1656 Linden Dr
contact.department: Pathobiological Sciences
contact.country: USA
contact.institute: University of Wisconsin - Madison
organism: Brucella melitensis
manufacture_protocol: Oligonucleotides (70-mers) representing 289 genes (Qiagen) were re-suspended in 3x SSC and spotted onto Ultra GAPS coated slides (Corning) using the MicroGrid Microarrayer (Apogent Discoveries). Slides were dessicated in a vaccum oven with Drierite for 48 hours and UV cross linked using a Stratalinker at 600mJ.  The printing of each ORF spot was verified by hybridizing with Cy3 labeled 9 mer (Qiagen).