title: Lieb Lab at UNC-CH_Yeast Oligo Tiling Array from (RandoLab_Harvard)
status: Public on Aug 11 2006
contact.city: Chapel Hill
contact.first: Jason
contact.middle: D
contact.last: Lieb
contact.email: jlieb@bio.unc.edu
contact.state: NC
contact.address: 203 Fordham Hall
contact.department: Department of Biology
contact.country: USA
contact.institute: University of North Carolina at Chapel Hill
contact.phone: 919-842-3228
organism: Saccharomyces cerevisiae
description: The sequence from S. cerevisiae chromosome III was fragmented into 50-mers overlapping every 20 base pairs. Potentially cross-hybridizing probes were filtered by BLASTing these sequences against the entire genome and eliminating sequences from chromosome III that included 5 or more contiguous probes with perfect BLAST matches, leaving 30 contigs covering 278,960 base pairs. Oligonucleotides were also tiled over one kilobase of 230 promoters on other chromosomes, starting at -900 from the start codon and extending to +100.
manufacture_protocol: MGuide, www.microarrays.org