title: Pathogen Chip
status: Public on Jan 01 2006
contact.city: Bethesda
contact.first: Concepcion
contact.last: Conejero-Goldberg
contact.email: goldbergc@stanleyresearch.org
contact.state: MD
contact.address: 8901 Wisconsin Avenue
contact.country: USA
contact.institute: Stanley Medical Research Institute
contact.phone: 301-295-1248
organism: Viruses
description: Synthetic long oligonucleotide probes spotted in duplicate on glass. The platform includes 567 oligonucleotides derived from specific viruses. Viral oligonucleotides were designed based on open reading frames from highly conserved and heterogenic regions within viral families. Viral genes reflecting different stages of infection were also included, to potentially define the stage of viral infection. The platform also includes 17 oligonucleotides from parasites, 11 oligonucleotides from bacterial species and 120 oligonucleotides from housekeeping human genes.
manufacture_protocol: Synthetic oligonucleotide probes (Operon-QIAGEN, Stanford, CA) were solubilized in 3X SSC at a concentration of 50 pmol/ml and spotted in duplicate on each Poly-L-Lysine coated glass slide (http://nciarray.nci.nih.gov/reference/NCIReference.shtml). Each array was configured in 4 x 20 x 20 format and post processed using standard procedures (http://nciarray.nci.nih.gov/reference/NCIReference.shtml).