title: HAC Human 2.3K
status: Public on Oct 01 2010
contact.city: São Paulo
contact.first: Renato
contact.middle: David
contact.last: Puga
contact.state: São Paulo
contact.address: Dr Ovidio Pires de Campos,785
contact.department: Instituto de Psiquiatria
contact.country: Brazil
contact.institute: Universidade de São Paulo
organism: Homo sapiens
manufacture_protocol: Glass arrays with 2,352 elements were prepared with the aid of the Flexis Robot (Genomic Solutions, UK). Bacteria were grown in LB medium containing ampicillin (250mg/ml). Inserts were amplified by PCR using M13 reverse and forward primers and products were submitted to 1% agarose gel for quality control. cDNA clones with single amplicons were filtrated in Sephadex G50 (GE Healthcare, UK), printed onto Corning glass slides (Corning, USA) and fixed by UV crosslink (Eisen & Brown, 1999; Brentani et al., 2005). There were 1364 unique genes in the array. Those genes were defined trough literature search for genes related to
  neurodevelopmental pathways.