title: cDNA microarray Trymanosoma cruzi - v.2
status: Public on Jun 13 2005
contact.city: Ribeirao Preto
contact.first: Ricardo
contact.middle: Z.N.
contact.last: Vêncio
contact.state: SP
contact.address: Av. Bandeirantes, 3900
contact.department: Computing and Mathematics Department
contact.country: Brazil
contact.institute: Universidade de São Paulo
organism: Trypanosoma cruzi
description: DNA microarrays were constructed with 755 ESTs obtained from non-normalised and normalised cDNA libraries of CL Brener epimastigotes and from amastigotas of Tulahuen (Ürmenyi et al., 1999; DaRocha et al., 2000). Polymerase chain reaction (PCR) amplification of the ESTs (average length 800 bp) was obtained with T3 and T7 primers using DNA polymerase (Biolase). The amplification products were purified with Multiscreen plates (Millipore). Inserts representing 32 cloned T. cruzi coding and non-coding genes, control sheared DNAs and oligonucleotides were also immobilised on the glass slides with Generation III Microarray System spotter (Molecular Dynamics) according to manufacturer's instructions.