title: IQUSP Human Intron-Exon 44k v3.0
status: Public on Jun 30 2015
contact.city: São Paulo
contact.first: Eduardo
contact.middle: Moraes
contact.last: Reis
contact.email: emreis@iq.usp.br
contact.state: SP
contact.address: Av. Prof. Lineu Prestes, 748
contact.department: Biochemistry
contact.country: Brazil
contact.institute: University of São Paulo
contact.phone: +55-11-30912173
organism: Homo sapiens
description: Assembly Hg19 was used for genomic coordinates of the probes.
manufacture_protocol: Agilent’s non-contact industrial inkjet printing process uniformly deposits oligo monomers onto specially-prepared glass slides. Custom microarrays are manufactured using Agilent’s non-contact in situ synthesis process of printing 60-mer length oligonucleotide probes, base-by-base, from digital sequence files. This is achieved with an inkjet process which delivers extremely small, accurate volumes (picoliters) of the chemicals to be spotted. Standard phosphoramidite chemistry used in the reactions allows for very high coupling efficiencies to be maintained at each step in the synthesis of the full-length oligonucleotide. Precise quantities are reproducibly deposited “on the fly.” This engineering feat is achieved without stopping to make contact with the slide surface and without introducing surface-contact feature anomalies, resulting in consistent spot uniformity and traceability.