title: Stanford Caulobacter crescentus 4.1K printing_AB
status: Public on Mar 10 2010
contact.city: East Lansing
contact.first: Sean
contact.last: Crosson
contact.email: crosson4@msu.edu
contact.state: Michigan
contact.address: 567 Wilson Rd
contact.department: Dept. Microbiology and Molecular Genetics
contact.country: USA
contact.institute: Michigan State University
contact.phone: 5178845345
organism: Caulobacter vibrioides
description: Spotted oligonucleotide array on aldehyde coated glass.
  The array contains at least 1 50-mer probe for each ORF in Caulobacter crescentus.
manufacture_protocol: The microarrays for this study were printed using the GeneMachines Omnigrid Microarrayer with Telechem 16-pin ArrayIt Stealth MicroSpotting Pins. Oligo sequences were chosen to satisfy the following criteria: be a unique sequence in the genome, be located near the 5’ end of the gene, approximately 100 base pairs downstream of the predicted start codon, have similar melting temperatures, and have minimal predicted secondary structure formation. The oligos were modified at the 5’ end with a six-carbon spacer followed by a primary amino group and diluted to 20µM for printing. QMT Aldehyde slides and QMT Spotting Solution I were obtained from Quantifoil.