title: FHCRC Human 18K v1.0
status: Public on Feb 17 2005
contact.city: Seattle
contact.first: Ryan
contact.last: Basom
contact.state: WA
contact.address: 1100 Fairview Ave N
contact.country: USA
contact.institute: Fred Hutchinson Cancer Research Center
organism: Homo sapiens
description: Microarray construction and hybridization protocols were modified from DeRisi, J.L., V.R Iyer, and P.O. Brown. Science 278:680-686, 1997. The microarrays were constructed using a set of more than 17000 sequence-verified clones from Research Genetics (UniGene Build 133). The cDNA inserts were amplified from replica plate cultures by PCR, purified and mechanically spotted onto polylysine-coated microscope slides using an OmniGrid robotic spotter (GeneMachines, San Carlo, CA).