title: yeast_nucleosome_positioning_microarray 6-9
status: Public on Sep 01 2005
contact.city: Worcester
contact.first: Oliver
contact.last: Rando
contact.email: Oliver.Rando@umassmed.edu
contact.state: MA
contact.address: 364 Plantation St.
contact.country: USA
contact.institute: UMass Medical School
contact.phone: 508-856-8879
organism: Saccharomyces cerevisiae
description: The sequence from S. cerevisiae chromosome III was fragmented into 50-mers overlapping every 20 base pairs. Potentially cross-hybridizing probes were filtered by BLASTing these sequences against the entire genome and eliminating sequences from chromosome III that included 5 or more contiguous probes with perfect BLAST matches, leaving 30 contigs covering 278,960 base pairs. Oligonucleotides were also tiled over one kilobase of 230 promoters on other chromosomes, starting at -900 from the start codon and extending to +100.  This platform contains two extra plates compared to Platform GPL1950.
  Keywords = nucleosome
  Keywords = yeast
  Keywords = tiling microarray
manufacture_protocol: MGuide, www.microarrays.org