title: Yeast Splicing/Expression Chip v6.2-6.3
status: Public on Apr 05 2011
contact.city: Santa Cruz
contact.first: Lily
contact.last: Shiue
contact.email: lshiue@ucsc.edu
contact.state: CA
contact.address: 1156 High Street
contact.department: MCD Biology
contact.country: USA
contact.institute: UCSC
contact.phone: 831-459-2612
organism: Saccharomyces cerevisiae
description: This is an oligonucleotide microarray designed to measure splicing and expression in yeast. Each intron containing gene has 3 features (Splice Junction, Intron, Exon2). The SJ oligo symmetrically spans the splice junction with 20nt on each side. The SJ, Int, and Ex2 oligos are custom designed 40-mers. The array also contains the Operon Yeast Genome 70-mer Set. All of the SJ, Int, and Ex2 oligos are printed 4 times, the Operon 70-mers are printed twice each.
  Keywords = Splicing and Expression
  Supplementary files: GAL files representing 2 different print layouts.
manufacture_protocol: DeRisi style arrayer;
  Printed on CodeLink slides;
  5' amine modified oligonucleotides were printed in phosphate buffer onto CodeLink slides;
  Slides were processed to remove unbound oligo.