title: Phalanx Escherichia coli 0.155 K Custom Small RNA Array
status: Public on Jun 15 2010
contact.city: Hsin-Chu
contact.first: Chi-Dung
contact.last: Yang
contact.email: eden1212.bt92g@nctu.edu.tw
contact.address: No 75, St. Po-Ai
contact.department: Department of Biological Science and Technology
contact.country: Taiwan
contact.institute: National Chiao Tung University
organism: Escherichia coli
description: Phalanx Escherichia coli Custom Small RNA Array is 1 in. X 3 in. (25 mm x 75 mm) standard-format glass slide. Probe is spotted onto the array in a highly consistent manner using an innovative non-contact spotting technology adapted and perfected by Phalanx Biotech for microarray manufacturing.
manufacture_protocol: The sequence of sRNAs and control genes were selected from Ecocyc and Ecogene Database. All of the 50 mer oligonucleotide sequences were design and determined by by ArrayDesign 4.2. Each of the probes was a perfect match to the corresponding E.coli chromosomal sequences. The 155 Probes small RNA array was made by Phalanx Biotech Group. The pre-synthesized DNA oligonucleotides with 5’ amine modification (Biosearch, CA, USA) were spotted and fixed onto the 1’x3’ standard glass by amide formation. To ensure the accuracy, each tri-spot was arranged in a stagger format to form a 9 replication in an 8.7mm*21.1mm printing area. Each glass slide contained 2 such printing areas for any experimental pair.
  See http://www.phalanxbiotech.com/ for updated protocols