title: BC009
status: Public on Aug 24 2013
contact.city: Princeton
contact.first: Bess
contact.middle: B
contact.last: Ward
contact.email: bbw@princeton.edu
contact.state: NJ
contact.address: Washington Road
contact.department: Geosciences
contact.country: USA
contact.institute: Princeton University
contact.phone: 6092585150
organism: artificial sequences
manufacture_protocol: 1.The probe oligonucleotides are printed onto Corning Ultragaps or Erie Scientific Epoxy Silane slides using a DeRisi style arrayer (DiRisi et al., 1997) and pins from Parallel Synthesis.
  2.The 70-mer oligo probes (Operon Technologies) were adjusted to a concentration of 0.05 mg ml-1 in 50%  DMSO and were spotted on CMT-GAPS amino silane-coated  glass slides (Corning, Corning, NY).
  3. After printing, the slides were baked at 80°C for 3 h and stored in the dark at room temperature under slight vacuum in a dessicator.