title: Agilent-031723 Sparus aurata SAPD_v.3 (Feature Number version)
status: Public on May 22 2014
contact.city: Legnaro (PD)
contact.first: Serena
contact.last: Ferraresso
contact.email: serena.ferraresso@unipd.it
contact.state: Padova
contact.address: Viale dell'Università
contact.department: Dept of Comparative Biomedicine and Food Science
contact.country: Italy
contact.institute: University of Padova
contact.phone: +39 049 8272506
organism: Sparus aurata
description: GE 8x15k oligo microarray that covers 12,879 annotated transcripts of the gilthead sea bream (Sparus aurata). Oligonucleotides, 60-mer plus a linker, are synthesized directly on a glass slide using Agilent's SurePrint Technology. A total of 8,143 probes were already employed on a previous version of the microarray (GPL6467) and maintained the same ProbeName (ContigName plus suffix _1 or _2). To them, one probe (ContigName without any suffix) for 4,002 transcripts with known orientation was added. In addition, 2 probes with different orientation (sense and antisense) were designed for transcripts with unknown orientation. Both independent probes designed for the same transcript are named with an univocal ID; the sense probes are distinguished by suffix_S, while the anti-sense probes are distinguished by suffix _AS. For probes employed on GPL6467, the sequence and description of each gene (contig) are available in the SAPD (Sparus aurata PaDova) database (http://enne.cribi.unipd.it:5555/biomart/martview ; described in Ferraresso et al. 2008 (PMID 19055773)), where contig ID corresponds to probe ID on the array.
manufacture_protocol: See SurePrint Technology at manufacturer's web site (www.agilent.com).