title: Arabidopsis 0.26K cDNA macroarray
status: Public on Oct 28 2012
contact.city: Poznan
contact.first: Agnieszka 
contact.middle: Wioletta
contact.last: Ludwików
contact.email: ludwika@amu.edu.pl
contact.address: Umultowska 89
contact.department: Biotechnology
contact.country: Poland
contact.institute: Adam Mickiewicz University
organism: Arabidopsis thaliana
manufacture_protocol: The A. thaliana EST probes were spotted on nylon membranes using a 384-manual spotting tool (Cirad Genomics Platform/Biotrop; Montpellier). The probes were spotted in duplicates. The membranes were rinsed in 2 x SSC buffer, dried and the DNA permanently fixed in an UV cross-linker. The control hybridization was carried out with radioactively labeled T7 primer for verification of a quality. After hybridization membrane was exposed to the PhosphorImager and the hybridized signals were analyzed by using a BioImaging analyzer.