title: MRA-1038B_miRPlants_16
status: Public on Dec 31 2013
contact.city: hangzhou
contact.first: miao
contact.last: dong
contact.email: 412789494@163.com
contact.address: No.2 street
contact.country: China
contact.institute: Zhejiang Sci-Tech University
organism: Pinellia pedatisecta
  Pinellia ternata
manufacture_protocol: 1. Arrays are made based µParaflo microfluidic technology (Atactic Technologies).
  2. On the µParaflo  microfluidic chip, each detection probe consists of a chemically modified nucleotide coding segment complementary to target microRNA (from miRBase,  http://www.mirbase.org/) or other RNA).
  3. A spacer segment of polyethylene glycol to extend the coding segment away from the substrate.
  4. The detection probes are made by in situ synthesis using PGR (photogenerated reagent) chemistry.