title: Human Epigenome Microarray Platform V1.0
status: Public on Jan 26 2012
contact.city: Stanford
contact.first: Chih Long
contact.last: Liu
contact.state: CA
contact.address: 269 Campus Drive
contact.department: Medicine - Imm. & Rheum
contact.country: USA
contact.institute: Stanford University
organism: Homo sapiens
manufacture_protocol: Synthetic peptides were custom-synthesized from the Yale Keck Biotechnology Resource Library with the sequences as described in the data table. Peptides were resuspended in 0.4 mg/ml in 0.5X phosphate-buffered saline (PBS) pH 7.4 with 2.5% glycerol and 0.025% tween-20. Custom microarrays were printed with the Bio-Rad Chip Writer Pro on ArrayIt streptavidin-coated slides, with each peptide printed in 6 replicates. Arrays were stored vacuum-packed for up to 2 months at 4 degrees Celsius before use.