title: LC_MRA-1038_miRPlants_15
status: Public on Oct 22 2013
contact.city: Houston
contact.first: Christoph
contact.last: Eicken
contact.email: ceicken@lcsciences.com
contact.state: TX
contact.address: 2575 W. Bellfort
contact.country: USA
contact.institute: LC Sciences, LLC
contact.phone: +1 (713) 664-7087
organism: Physcomitrium patens
  Picea glauca
  Picea sitchensis
  Pinus taeda
  Liriodendron tulipifera
  Persea americana
  Populus tremuloides
  Populus trichocarpa
  Populus trichocarpa x Populus deltoides
  Malus domestica
  Prunus persica
  Lupinus luteus
  Medicago truncatula
  Phaseolus vulgaris
  Phaseolus coccineus
  Manihot esculenta
  Ricinus communis
  Nicotiana sylvestris
  Nicotiana tabacum
  Nicotiana benthamiana
  Petunia x hybrida
  Ipomoea batatas
  Lactuca sativa
  Hordeum vulgare
  Oryza rufipogon
  Oryza sativa
  Oryza brachyantha
  Oryza nivara
  Cenchrus americanus
  Sorghum bicolor
  Lolium arundinaceum
  Phleum pratense
  Lotus japonicus
  Cenchrus ciliaris
  Ipomoea nil
  Prunus armeniaca
  Peridictyon sanctum
  Panicum virgatum
  Populus tremula x Populus tremuloides
  Rorippa indica
  Oryza barthii
  Nasturtium officinale
  Nuphar advena
  Prunus salicina
  Hordeum vulgare subsp. vulgare
  Populus tremula
  Oenocarpus bataua
  Malcolmia maritima
manufacture_protocol: plant miRNA microarray with 100% coverage of mature plant miRNAs listed in miRBase version 15.0. Arrays are made based µParaflo microfluidic technology (Atactic Technologies). On the µParaflo microfluidic chip, each detection probe consists of a chemically modified nucleotide coding segment complementary to target microRNA (from miRBase, http://miRBase.org) or other RNA (control or customer defined sequences) and a spacer segment of polyethylene glycol to extend the coding segment away from the substrate. The detection probes are made by in situ synthesis using PGR (photogenerated reagent) chemistry.