title: Agilent Mouse 4X44K plus 2500 MEEBO
status: Public on Aug 17 2008
contact.city: San Francisco
contact.first: Andrea
contact.middle: J
contact.last: Barczak
contact.state: CA
contact.address: 1550 4th St Rock Hall 545
contact.country: USA
contact.institute: UCSF Sandler Center Functional Genomics Core Facility
organism: Mus musculus
description: This array contains the following:
  1. The entire Agilent whole genome mouse 4x44K probe set  (design# 014868)
  2. 2500 MEEBO sequences that were not represented in the Agilent set (randomly selected from a larger group of ~3000)
  3. MEEBO probes for tectonic, kif2a, OFD1. The Agilent probe set also contained sequences for these genes.
  4. All transgene probes from the MEEBO set
  5 Probes designed from the full length sequences for tectonic 2 and 3 ( these were not represented on the MEEBO or Agilent arrays). There are 2 probes for tectonic 3 (splice variants).
  Additional probes are replaced the “empty” slots on the original Agilent mouse whole genome chip.
manufacture_protocol: Agilent’s non-contact industrial inkjet printing process uniformly deposits oligo monomers onto specially-prepared glass slides. Both the catalog and custom microarrays are manufactured using Agilent’s non-contact in situ synthesis process of printing 60-mer length oligonucleotide probes, base-by-base, from digital sequence files. This is achieved with an inkjet process which delivers extremely small, accurate volumes (picoliters) of the chemicals to be spotted. Standard phosphoramidite chemistry used in the reactions allows for very high coupling efficiencies to be maintained at each step in the synthesis of the full-length oligonucleotide. Precise quantities are reproducibly deposited “on the fly.” This engineering feat is achieved without stopping to make contact with the slide surface and without introducing surface-contact feature anomalies, resulting in consistent spot uniformity and traceability.