title: UIUC Bos taurus 13.2K 70-mer oligoarray (condensed)
status: Public on Aug 01 2010
contact.city: Corvallis
contact.first: Massimo
contact.last: Bionaz
contact.email: massimo.bionaz@oregonstate.edu
contact.state: OR
contact.address: 316A Withycombe Hall
contact.department: Animal and Rangeland Sciences
contact.country: USA
contact.institute: Oregon State University
contact.phone: 5417379507
organism: Bos taurus
description: The 13,257 oligos used in this microarray were obtained from normalized and subtracted cattle placenta and spleen cDNA libraries and based upon the earlier cDNA array platform GPL2864 (Everts et al., 2005 Vet. Immunol. Immunopathol. 105:235-245) and subtracted cDNA libraries created from embryonic (day 36 and day 64) and extra-embryonic (day 14 to 25) tissues (NCBI libraries 15993,15993, and 17188). Positive controls include beta actin (ACTB), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and hypoxanthine phosphoribosyltransferase (HPRT). Exogenous spiking controls are the soybean genes chlorophyll ab binding protein (CAB), Rubisco small chain 1 (RBS1), and major latex protein (MSG). Negative controls are Cot1 DNA, genomic DNA, spotting buffer, poly-A, and water. All 70-mer oligos were printed in duplicate on the array.  Keywords: oligo, cow, placenta, bovine, embryo, microarray
manufacture_protocol: 70-mer oligos were printed in 150 mM Phosphate buffer at 20 uM concentration.