title: Wilson human antibody microarray H3/H7
status: Public on Nov 14 2014
contact.city: CHICAGO
contact.first: Patrick
contact.middle: C
contact.last: Wilson
contact.email: wilsonp@uchicago.edu
contact.state: Illinois
contact.address: 924 E 57th street, Knapp building
contact.department: Medicine, Rheumatology
contact.country: USA
contact.institute: University of Chicago
contact.phone: 17737029009
organism: Homo sapiens
description: Human monoclonal antibodies spotted.
  The mAbs have been isolated from different individuals.  Some antibodies are from the same individual (i.e., the ones starting by the same 3 numbers, for example 011) but they have been cloned from different cells (we isolate single B cells by flow cytometry and clone the heavy and light chain to make the mAbs).
manufacture_protocol: 1. 6-8µl of each human monoclonal antibodies at 500µg/ml are deposited in a 384 wells plate (ArrayIt)
  2. the same volume of 2X Printing Buffer (ArrayIt) is added in each well. Mix well.
  3. antibodies are then printed, in triplicate, on SuperEpoxy treated glass slides (ArrayIt). The same set of antibody is spotted 24 times on the same slide allowing 24 samples to be applied.
  4. After the run, slides are kept at 4 degres before use.