title: Medicago truncatula 6K array
status: Public on Oct 14 2005
contact.city: St Paul
contact.first: Kathryn
contact.middle: A
contact.last: VandenBosch
contact.email: vande102@umn.edu
contact.state: MN
contact.address: Room 250 BioSci 6022 1445 Gortner Ave
contact.department: Plant Biology
contact.country: USA
contact.institute: University of Minnesota
contact.phone: +1 612-624-2755
organism: Medicago truncatula
description: Clones selected for the array were obtained from a wide variety of cDNA libraries that were previously utilized for Expressed Sequence Tag (EST) sequencing, and were
  obtained from the University of Minnesota and the Noble Foundation. We utilized the
  publicly available tentative consensus sequences (TCs) from The Institute of Genomics
  Research's Medicago Gene Index (http://www.tigr.org/tdb/mtgi/, Quackenbush et al.,
  2001), and Minnesota Contigs (MNCs) from the Center for Computational Genomics and
  Bioinformatics of the University of Minnesota (Lamblin et al., 2003) to select 6,144
  clones to be included on the cDNA microarray. All selected clones represented the 5'-
  most clone in a contig with maximal base call identity to the contig consensus. Clones selected for the array included those from the same contig as those present on the kiloclone array (Penuela et al., in preparation), clones from contigs that were verified to be free of chimerism and assembled consistently in both the TC and the corresponding MNC, and clones from the same contig as those present in a sample long oligo set marketed by Operon, to enable comparisons with experiments using that data.
  The project was supported by the NSF Plant Genome Project (award #0110206)
manufacture_protocol: arrayer hardware-
  Gene Machine OmniGrid array spotter