title: Univerisity of Wisconsin Gene Expression Center_Escherichia coli_4.2K_Eco15
status: Public on Jan 22 2008
contact.city: Northampton
contact.first: Christine
contact.middle: Ann
contact.last: White-Ziegler
contact.email: cwhitezi@smith.edu
contact.state: MA
contact.address: Clark Science Center
contact.department: Biological Sciences
contact.country: USA
contact.institute: Smith College
contact.phone: 413-585-3815
organism: Escherichia coli
manufacture_protocol: Microarray consists of full-length PCR products from all 4,290 annotated open reading frames (ORFs) in E. coli mg1655 (1).  PCR products are printed in replicate on standard, 1” X 3”, poly-l-lysine coated glass microscope slides.  Greater than 95% have been shown to migrate as a single band, of the predicted molecular weight, as determined by gel electrophoresis and ethidium bromide staining.  Also printed on each microarray are >200 control spots consisting of: fragmented E. coli genomic DNA, non-specific salmon sperm DNA, 3X SSC, and >25 different yeast ORF PCR products (see master table). These microarrays are printed using a 16-pin configuration, resulting in a pattern of 32 sub-arrays each having geometry of 17 rows by 18 columns with spot diameter averaging 100 microns. To aid in locating the array, the top and bottom array boundaries are etched on the back of the microscope slide.  A unique serial number is also etched on the array side at the bottom of the slide (see diagram in master table). The integrity and quality of each set of microarrays have been verified by hybridization with fluorescent-labeled cDNA and genomic DNA.