title: UHN Human 10K v1
status: Public on Dec 27 2012
contact.city: College Station
contact.first: Leslie
contact.middle: Garry
contact.last: Adams
contact.email: gadams@cvm.tamu.edu
contact.state: TX
contact.address: 402 Raymond Stotzer Blvd.
contact.department: Veterinary Pathobiology
contact.country: USA
contact.institute: Texas A&M University
contact.phone: 979-845-9816
organism: Homo sapiens
manufacture_protocol: 1. Acquisition of bacterial clone set—These are well characterized human ESTs, and annotations for most clones in this clone set are available in OMIM, RefSeq and/or SwissProt IDs.  2. Clone set is PCR amplified and DNA purified in working 96 well plates.  3. The purified DNA is then transferred to 384 well source plates with DNA concentration ranging from .10ug/ul to 0.25ug/ul. These are used for spotting.  4. We use high precision robots that can use up to 48 pins or print heads. H10k slides are printed using 48 pins and we use UltraGAPS slides (Corning Inc.) We are able to print up to 120 slides in each batch and batches that result in greater than 95% printing fidelity are selected for post printing quality control tests.  5. Post Printing Quality Control tests include: A real hybridization using commercially available RNA; Vector Probe labeling method; Terminal Transferase tailing method.