title: LC Sciences Soybean miRNA Array
status: Public on Aug 31 2013
contact.city: Guangzhou
contact.first: Xiaolong
contact.last: Fang
contact.email: fangxiaolong1215@163.com
contact.address: NO. 483 Wushan Road Tianhe District
contact.country: China
contact.institute: College of Agriculture, South China Agricultural University
contact.phone: 13560358425
organism: Glycine max
description: version: miRSoybean_SCAU_120308 array
  part no. MRA-2001
  The array design was based on 694 conserved miRNA mature sequences of legumes downloaded in miRBase Release 18.0 (http://www.mirbase.org/) and 259 well characterized miRNAs published in papers (Barrera-Figueroa et al., 2011; Chen et al., 2012; Li et al., 2011; Zeng et al., 2012; Zhang et al., 2008; Zhou et al., 2012b).
manufacture_protocol: On the microfluidic chip, each detection probe consisted of a chemically modified nucleotide coding segment complementary to target microRNA (from miRBase, http://www.mirbase.org/) or other RNA (control or customer defined sequences) and a spacer segment of polyethylene glycol to extend the coding segment away from the substrate. The detection probes were made by in situ synthesis using PGR (photogenerated reagent) chemistry.