title: DIPROVAL - OPERON Sus scrofa AROS V1.0
status: Public on Oct 08 2008
contact.city: Reggio Emilia
contact.first: Roberta
contact.last: Davoli
contact.email: roberta.davoli@unibo.it
contact.address: Via Fratelli Rosselli 107 -Villa Levi- Coviolo-
contact.department: Dipartimento di Protezione e Valorizzazione Agroalimentare
contact.country: Italy
contact.institute: University of Bologna
contact.phone: +390522290507
organism: Sus scrofa
description: This work was supported by a grant from FIRB of the Ministry of Education, University and Research (MIUR) of Italy –Scientific responsible person: Prof. Vincenzo Russo
manufacture_protocol: The Array-Ready Pig Genome Oligo Set Version 1.0 from Operon Biotechnologies was dissolved in 3XSSC and spotted using a MicroGrid II (BioRobotics) microarrayer onto Micromax SuperChipI glass slides (Perkin Elmer Life Sciences). The oligos were crosslinked (600 mJoules) onto the glass using a Stratalinker UV crosslinker (Stratagene).
  Post-spotting treatment: To eliminate salts and materials unlinked the following washes were utilised :
  1- 1 % SDS and 3X SSC (1’)by shaking
  2- Distilled water (5’) by shaking
  3- Boiling distilled water (30”)
  Additional information for the oligos is available via the Download Center at Operon Biotechnologies. Present annotation is based on data from OPERON Biotechnologies, Sus scrofa (pig) AROS V1.0, genelist version 1.0.5.