title: UCSF Drosophila melanogaster 7.8K spotted array
status: Public on May 20 2006
contact.city: San Francisco
contact.first: Julie
contact.last: Hollien
contact.email: juliehollien@yahoo.com
contact.state: CA
contact.address: 1700 Fourth Street
contact.department: Cell and Molecular Pharmacology
contact.country: USA
contact.institute: UCSF
organism: Drosophila melanogaster
manufacture_protocol: These spotted microarrays contain targets in the RNAi library described by Foley et al. (1), which consists of 7,216 phylogenetically conserved Drosophila genes. We also added to the library targets identified in Flybase (2) as being localized to the ER and targets whose expression in S2 cells changed upon the addition of DTT, as determined in pilot experiments using Affymetrix arrays. The targets are composed of exon sequences of 250-800 nucleotides, which we amplified by PCR, checked by agarose gel electrophoresis, and spotted onto poly-lysine coated slides as described (3). The primer sequences are contained in the platform file.
  1.	E. Foley, P. H. O'Farrell, PLoS Biol 2, E203 (Aug, 2004).
  2.	R. A. Drysdale, M. A. Crosby, Nucleic Acids Res 33, D390 (Jan 1, 2005).
  3.	J. L. DeRisi, V. R. Iyer, P. O. Brown, Science 278, 680 (Oct 24, 1997).