title: AECOM Human 28K HH5 series
status: Public on Sep 05 2012
contact.city: Bronx
contact.first: Antonia
contact.last: Patsialou
contact.email: antonia.patsialou@einstein.yu.edu
contact.state: NY
contact.address: 1301 Morris Park Ave
contact.department: Anatomy and Structural Biology
contact.country: USA
contact.institute: Albert Einstein College of Medicine
organism: Homo sapiens
manufacture_protocol: Information about the AECOM custom-printed chips can be found in http://microarray1k.aecom.yu.edu/.  Unique Genome Systems human full lenght cDNAs from the I.M.A.G.E. consortium were used for printing on glass slides.  The arrays were printed under rigorous clean-room conditions and quality control is employed all along the way, starting with preparation of the printing material as well as during the actual printing and finally with post-printing tests that include a sample chip hybridization. The microarray printer consists of a three-axis robot mounted on a Newport Vibration Isolation Workstation table with a research-grade laser tabletop.  The three-axis mechanical slides are from THK and are selected to provide the best combination possible of precision and speed consistent with the design objectives. The Pen Tip Assembly consists of a proprietary design having 12 pen tips contained within a precision-machined housing that is supported by the moving block on the Z-axis slide.  The mechanical slides are positioned with motors and servo amplifiers from Yaskawa. The robot can spot 12 points every 1/2 second, or, 100 slides with 12 spots each in under 60 seconds.   The resulting slides of the HH5 series had the following configuration: HUMAN 27K gene chips: 48 grids, 26 columns X 23 rows each.