title: UCLA-MEMLAB HP 1543 Version 2
status: Public on Sep 14 2009
contact.city: Los Angeles
contact.first: Yi
contact.last: Wen
contact.email: ywen@ucla.edu
contact.state: CA
contact.address: 11301 Wilshire Blvd., Bldg.113, Rm. 324
contact.department: Physiology Department
contact.country: USA
contact.institute: UCLA/VAGLAHS
contact.phone: 310-268-4672
organism: Helicobacter pylori
manufacture_protocol: 1. PCR products that contained 1,534 predicted open reading frames (ORFs) of Helicobacter pylori strain 26695 were purchased from ResGen-Invitrogen Corporation.
  2. The concentration of PCR product was estimated based on the density of the band on ethidium bromide-stained agarose gel.
  3. After purification of the PCR products using QIAquick 96-well PCR purification kit (Qiagen), ~4 µg of PCR product for each ORF was loaded into a fresh 384-well Flex Chips Source plate provided by TeleChem International, Inc.
  4. After completely dry the plates with Speed-Vacuum, the dried DNA plates were re-suspended by adding 5 µl of Micro Spotting Plus (ArrayIt, Cat.# MSP) to each well for printing (TeleCom Internalional, Inc. ArrayIt).