title: HGMP NIA 15K set, format 2
status: Public on Aug 15 2007
contact.city: Cardiff
contact.first: Fiona
contact.middle: Catherine
contact.last: Mansergh
contact.email: mansergf@tcd.ie
contact.state: Wales
contact.address: Museum Avenue
contact.department: Biosciences
contact.country: United Kingdom
contact.institute: Cardiff University
contact.phone: 0035318962484
organism: Mus musculus
description: HGMP printed NIA 15K arrays, printed on 2 slides, 17280 spots per slide, including control spots, empties and landmarks. 2 duplicate spots per slide for each clone. Earlier versions of these slides have already been described by platform entries GPL5457 and GPL5458. However, owing to analysis of a later array print run by a different image analysis programme in this series of experiments, the data files present in a different clone order and it is impossible to sort them to generate the same order. Hence this new platform submission. For ease of analysis, this platform entry combines Slides one and Two, (which are always hybed in pairs with the same hybe solution anyway)
manufacture_protocol: cDNA arrays (now discontinued), spotted PCR product. Bacterial source material diluted 1/10 prior to PCR,
  primers: Ko forward primer (amidated): GTG TGG AAT TGT GAG CGG ATA ACA A (25mer) with 5' NH2(C6) modification.
  Ko reverse primer: CCA GTC ACG ACG TTG TAA AAC GAC (24mer). Purified using Millipore Multiscreen PCR plates. Purified PCR products have then been rearrayed into 384 well plates and printed. 20µl of spotting buffer is added to 10µl of PCR product (0.1ug/ul) and this is sufficient to grid over 1000 glass arrays.
  The manufacturers ask that the following paper describing the clones is cited in connection with these arrays: Tanaka TS, et al Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray. PNAS (2000) 97: 9127-32