title: Brucella 16M whole genome microarray
status: Public on Oct 27 2010
contact.city: Beijing
contact.first: Zeliang
contact.last: Chen
contact.email: zeliangchen.amms@gmail.com
contact.address: NO. 20,Dongdajie
contact.country: China
contact.institute: Beijing Institute of Disease Control and Prevention
organism: Brucella melitensis bv. 1 str. 16M
manufacture_protocol: 1.Primers specifc for each of the ORFs of 16M genome sequence are designed by Arraydesigner 3.0. Primers are synthesized in 96-well plates.
  2. Products of each of the ORFs were amplified in 96-well plate.
  3. PCR products are purified in 96-well plates.
  4. PCR products are disolved in 50% DMSO, gyrB, glk and 16s rRNA are included in each of the blocks as positive contrl, and 50% DMSO as negative control. These products are transferred into 384-well plates.
  5. Products are printed in duplicate on VAL S25 glass slides (ECL); Each slide is composed of 32 sub-arrays in 18 columns and 12 rows.
  6. After the print run is completed, allow plates to dry through passive evaporation in a protected environment.