title: Bovine Oligo array 25K
status: Public on Oct 15 2010
contact.city: Quebec
contact.first: Isabelle
contact.last: Gilbert
contact.email: isabelle.gilbert.4@ulaval.ca
contact.state: Quebec
contact.address: Pavillon INAF
contact.department: Sciences Animales
contact.country: Canada
contact.institute: Universite Laval
organism: Bos taurus
manufacture_protocol: 1.The Bovine oligo array has a total of 25, 200 oligonucleotides including unknown sequence, positive and negative control
  2.16,846 oligo sequences correspond to protein-encoding transcripts taken from the Bovine Oligo Microarray Consortium database (BOMC, http://www.bovineoligo.org).
  3. The oligos are 70-mer in length
  4. The array is printed in singlet using an Arrayjet Supermarathon instrument onto Corning GAPS II slides.
  5. The DNA is cross linked to each slide and blocked using a BSA block
  6. Each batch of slides is QC checked for the deposition of the oligonucleotides and a with test hybridisations using amplified RNA probes.