title: Eurogentec AG S. pombe ORF array 10k (1st batch)
status: Public on May 03 2006
contact.city: Huddinge
contact.first: Anthony
contact.last: Wright
contact.email: yongtao.xue.franzen@ki.se
contact.address: Blickagången 6
contact.country: Sweden
contact.institute: Karolinska Institute
organism: Schizosaccharomyces pombe
description: 4976 ORFs spotted in duplicate on glass slide
  Keywords = yeast ORF DNA microarray
manufacture_protocol: Primary PCR: The PCR reactions are performed using tagged genome specific primers in the presence of genomic DNA (provided by your laboratory). We normally achieve a success rate of 93 % of PCR reactions. For the failed PCR reactions we perform three different protocols of PCR conditions, which include different MgCl2 concentrations and different Taq polymerase. We should normally get an additional 2 % working and reach a success rate of 95 % in total. We check 5 % of the total oligos by Mass Spectrometry and will re-synthesize maximum 2 % of the oligos based on a newly designed primer. We will perform the PCR reactions with the new designed oligonucleotides with the standard PCR protocol. Following this process we reach usually 97 % success rate.