title: Spotting_muscle_21OCT03
status: Public on Aug 29 2006
contact.city: Castanet tolosan
contact.first: Laurence
contact.last: Liaubet
contact.email: laurence.liaubet@inra.fr
contact.address: chemin de borde rouge
contact.department: Animal Genetic
contact.country: France
contact.institute: INRA
organism: Sus scrofa
manufacture_protocol: Gene expression was analyzed by hybridization of nylon cDNA arrays (18x72 mm, Immobilon-NY+, Millipore, Bedford, MA)with radioactive probes. The arrays contained PCR products from 3456 pig cDNA clones, spotted in duplicate on two separate fields of the same membrane with aMicrogrid II fitted with a 32 pins microarray tool (BioRobotics MicroGrid, Genomic Solutions Ltd., 8 Blackstone Road, Huntingdon, Cambridgeshire PE29 6EF, United Kingdom). The cDNA clones came mainly from a pig normalized multi-tissue cDNA library (scag, Tosser-Klopp G, unpublished 1056 clones) and from a muscle library (Bendixen C, Department for Livestock Breeding and Genetics, Danish Institute of Agricultural Sciences, DK-8830 Tjele; 2208 clones). Other clones came from libraries from USDA MARC (MARC 1 and 2 pig) and SENAH INRA laboratory (INRA - UMR SENAH, Domaine de la Prise, F 35590 Saint Gilles, France).