title: CIMAP Artemisia annua (cv. CIM-Arogya) microarray platform
status: Public on Dec 31 2014
contact.city: Lucknow
contact.first: Ajit
contact.middle: K.
contact.last: Shasany
contact.email: akshasany@yahoo.com
contact.state: Uttar Pradesh
contact.address: P.O. CIMAP
contact.department: Biotechnology Division
contact.country: India
contact.institute: CSIR-Central Institute of Medicinal and Aromatic Plants
contact.phone: +91-522-2718548
organism: Artemisia annua
manufacture_protocol: Five microgram of total RNA was used for amplification using Amino Allyl MessageAmp™ II aRNA Amplification Kit from Ambion by linear transcription based RNA amplification system to produce cRNA. Briefly, mRNA was reverse transcribed with a oligo (dT) primer bearing T7 promoter at 42°C for 2 hrs and second strand cDNA synthesis was carried out at 16°C for 2 hrs. The resulting cDNA was then purified and was then transcribed with T7 RNA polymerase to generate multiple copies of aminoallyl antisense RNA (aRNA) at 37°C for 16 hrs. aRNA was then labelled with Cy3TM/Cy5TM post-labelling reactive dye pack (GE Healthcare, UK) at room temperature and unincorporated Cy3/Cy5  molecules were removed by purification process. Samples were purified using QIAGEN PCR purification kit before hybridization.