title: INSA Lactococcus lactis IL1403 (lot de PCR n°2)
status: Public on Apr 28 2009
contact.city: Toulouse
contact.first: Muriel
contact.last: Cocaign-Bousquet
contact.email: cocaign@insa-toulouse.fr
contact.address: 135 avenue de Rangueil
contact.department: UMR5504 INSA/CNRS - UMR792 INSA/INRA
contact.country: France
contact.web_link: http://www.insa-toulouse.fr/fr/recherche/labo/lisbp.html
contact.institute: Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés
organism: Lactococcus lactis
description: nylon membrane positively charged (Roche)
  8*12 cm
manufacture_protocol: Amplicons (PCR) were designed and amplified by Eurogentec. A mean length of 535 bp and final concentrations between 40 and 380 µg/l were obtained. PCR were diluted in DMSO 50 % in 384 wells spot plates and spotted (4 deposits/ spot) in duplicate on positively charged nylon membranes (Roche) with a Biogrid spotter (Biorobotics) with a 4*4 pattern.