title: VMSR Compugen Human Oligo P6G5
status: Public on Apr 29 2008
contact.city: Nashville
contact.first: Braden
contact.last: Boone
contact.state: TN
contact.address: 465 21st Ave South
contact.department: Vanderbilt Microarray Shared Resource
contact.country: USA
contact.institute: Vanderbilt University
organism: Homo sapiens
manufacture_protocol: Microarrays containing all oligonucleotides from the Compugen Human Release 2.0 OligoLibrary were resuspended in 150mMol phosphate buffer and arrayed according to the manufacturers recommendations.  Microarrays were produced by Microarrays Incorporated (www.microarrays.com) utilizing custom, high precision robotics and amine-coated glass slides from Genexprex.  The microarrays were then crosslinked using  80mjoules of ultraviolet energy (Stratagene Stratalinker, LaJolla, CA).  Following crosslinking, the arrays were baked for two hours at 70C in a standard oven and stored under low humidity conditions until use.