title: MiRPlants-12.0 plus 100 maize smRNAs
status: Public on Jan 06 2011
contact.city: Rosario
contact.first: Paula
contact.last: Casati
contact.email: casati@cefobi-conicet.gov.ar
contact.state: Santa Fe
contact.address: Suipacha 531
contact.department: Biological Sciences
contact.country: Argentina
contact.institute: Universidad Nacional de Rosario
contact.phone: 54-341-4371955
organism: Physcomitrium patens
  Pinus taeda
  Gossypium hirsutum
  Populus trichocarpa
  Arabidopsis thaliana
  Brassica napus
  Glycine max
  Medicago truncatula
  Solanum lycopersicum
  Oryza sativa
  Saccharum officinarum
  Sorghum bicolor
  Triticum aestivum
  Zea mays
  Vitis vinifera
  Selaginella moellendorffii
description: LC Sciences provides a genome-wide microRNA (miRNA) expression profiling service using µParaflo® technology and proprietary probe design, which enable highly sensitive and specific direct detection of miRNAs. They have standard arrays for mature miRNA of all species available in the latest version of the Sanger miRBase database.
  A proprietary µParaflo® microfluidic chip is used.   The microfluidic technology produces a uniform distribution of the sample solutions on the array and enhances binding reactions and stringency wash processes.   In situ oligonucleotide synthesis using PGA (photogenerated acid) coupled with conventional DMT chemistry means high probe quality, tight process control, and complete content flexibility.
manufacture_protocol: The miRNA microarray was custom synthesized by LC Sciences (Houston, Texas, USA) using their protocols (Gao et al., 2004).
  Gao, X., Gulari, E. and Zhou, X. (2004) In situ synthesis of oligonucleotide microarrays. Biopolymers, 73, 579–596.