title: Adelaide Microarray Facility CompuGen OligoLibrary Mouse 22k Hybrid of v1.0 and v2.1
status: Public on Mar 20 2006
contact.city: North Adelaide
contact.first: Christopher
contact.last: Wilkinson
contact.email: christopher.wilkinson@adelaide.edu.au
contact.state: South Australia
contact.address: 72 King William Road
contact.country: Australia
contact.institute: Child Health Research Institute
contact.phone: 618 8161 6363
organism: Mus musculus
description: 4x16 print groups, 24rows x 20 cols per print-tip group. this experiment covered two print runs with print run 1 layout used for slides AMC0374 - AMC0472 and print run 2 layout was used for slides AMC1000+. The layout was changes between print runs requiring remapping of run 1 spot positions to run 2 spot positions. For the CompuGen OligoLibrary, and the last 8 rows of column 1 this was a simple remapping. However for run 1, the Adelaide control plate was spotted twice and for run 1 it was spotted once. Thus spots in print run 2, rows 9-16 of col 1, are hybrids. In run 1 these were housekeeping genes or other controls, but in run 2 their equivalent spot was always buffer. In this platform description these are indicated by Hybrid in ID name.
manufacture_protocol: CompuGen Mouse OligoLibrary V2.0 comprising 21,991 65-mers representing 21,587 unique genes. Additional locally made control plate spotted. Spots printed onto Corning CMT-GAPS slides using a 4x16 pinhead robot. Each print head printed an array of 480 spots arranges as a 24 rows by 20 columns (23040 features per array). See www.microarray.adelaide.edu.au for printing details.