title: PFGRC Burkholderia mallei/pseudomallei 9.9K oligo array v2
status: Public on Oct 31 2020
contact.city: Honolulu
contact.first: Tung
contact.last: Hoang
contact.email: yunkang@hawaii.edu
contact.state: HI
contact.address: 1800 East-West Road
contact.department: Microbiology
contact.country: USA
contact.institute: University of Hawaii at Manoa
contact.phone: 808-956-5035
organism: Burkholderia mallei
  Burkholderia pseudomallei
manufacture_protocol: The array contains a total of 9,826 oligo probes (70-mer) consisting 5,174 oligos from B. mallei, 3,261 from B. pseudomallei 1710b and 1,391 from B. pseudomallei K96243 to which the design target belongs. These oligos correspond to a 100% sequence identity and 100% length hit with the locus given as the design target (more than one locus from different strains used in the design). Oligos are designed based on ORF sequence across all genomes for a given chip. All oligos represent multiple strains and/or species (5,440 ORFs of B. mallei, 5,854 ORFs of B. pseudomallei K96243 and 6,348 ORFs of B. pseudomallei 1710b). Each probe was spotted in duplication. There are more than one oligo from different strains many locus from many strain (the oligos that have multiple hits with 90-99.99 % identity correspond to any locus) that can be used a replicate and they were averaged their target. All arrays contained 48 grids (12 x 4 configurations). Each block has 21 rows and 22 columns. Surface coating is aminosilane. The receipt slides were stored in a humidity-controlled cabinet until used.