title: WUSTL Human Herpesvirus 1
status: Public on Apr 01 2008
contact.city: St. Louis
contact.first: Seth
contact.middle: Daniel
contact.last: Crosby
contact.email: scrosby@wustl.edu
contact.state: MO
contact.address: 660 S. Euclid
contact.department: Genetics
contact.country: USA
contact.institute: Washington Univeristy School of Medicine
contact.phone: 314-286-1256
organism: Human alphaherpesvirus 1
description: To construct a gene-specific microarray of the Human Herpesvirus 1 (Herpes simplex virus type 1) genome, we designed 70-mer oligonucleotide array elements.  Predicted and known HHV-1 genes were obtained from the strain 17 sequence on the NCBI  website (http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=NC_001806).  In addition, genes derived from mouse, common marker and selection genes, and mutant virus sequences were included.  Arabidopsis elements were included for normalization.  Oligonucleotides were designed from these sequences using ArrayOligoSelector.
manufacture_protocol: Oligos were synthesized using standard methods by Illumina (San Diego, CA). These oligonucleotides, as well as Stratagene control A. coli sense oligos #7 and #8 (Ambion part# 1781), were dissolved at a concentration of 50 μM in 3X SSC with 0.75M betaine and were printed in duplicate on MWG Epoxy slides by a locally constructed linear servo arrayer (after the DeRisi model, http://derisilabs.ucsf.edu/).