title: NimbleGen Zmays 120K CGH array [091006_NS_Zmays_CGH_exp]
status: Public on Oct 15 2010
contact.city: St. Paul
contact.first: Steve
contact.middle: R
contact.last: Eichten
contact.email: eicht021@umn.edu
contact.state: MN
contact.address: 1445 Gortner Ave
contact.department: Plant Biology
contact.country: USA
contact.institute: University of Minnesota
organism: Zea mays
description: product # OID24389
manufacture_protocol: Roche NimbleGen manufactures custom, high-density DNA arrays based on its proprietary Maskless Array Synthesizer (MAS) technology. The MAS system is a bench top, solid-state, high-density DNA array fabrication instrument comprised of a maskless light projector, a reaction chamber, a personal computer, and a DNA synthesizer. Roche NimbleGen builds its arrays using photo-mediated synthesis chemistry with its MAS system.    At the heart of the system is a Digital Micromirror Device (DMD), similar to Texas Instruments' Digital Light Processor (DLP), employing a solid-state array of miniature aluminum mirrors to pattern 786,000 to 4.2 million individual pixels of light. The DMD creates virtual masks that replace the physical chromium masks used in traditional arrays.    These virtual masks reflect the desired pattern of UV light with individually addressable aluminum mirrors controlled by the computer. The DMD controls the pattern of UV light projected on the microscope slide in the reaction chamber, which is coupled to the DNA synthesizer. The UV light selectively cleaves a UV-labile protecting group at the precise location where the next nucleotide will be coupled. The patterns are coordinated with the DNA synthesis chemistry in a parallel, combinatorial manner such that 385,000 to 2.1 million unique probe features are synthesized in a single array.