title: NanoString nCounter Gama2 Gene Expression Custom CodeSet
status: Public on Jul 01 2011
contact.city: Baltimore
contact.first: Lucio
contact.last: Gama
contact.email: lucio@jhmi.edu
contact.state: MD - EUA
contact.address: 733 N Broadway St - BRB 831
contact.department: Molecular and Comparative Pathobiology
contact.country: USA
contact.institute: Johns Hopkins School of Medicine
contact.phone: 410-955-9770
organism: Macaca nemestrina
description: Gama2 CodeSet is specific for inflammatory genes.
  Gama2 CodeSet Probe count: 92
  
  In brief, a multiplexed probe library is made with two sequence-specific probes (Capture and Reporter) for each gene of interest.  The first probe, a Capture probe, contains a 35- to 50-base sequence complementary to a particular target mRNA plus a short common sequence coupled to an affinity tag such as biotin. The second probe, the Reporter probe, contains a second 35- to 50-base sequence complementary to the target mRNA, which is coupled to a color-coded tag that provides the detection signal. The tag consists of a single-stranded DNA molecule, the backbone, annealed to a series of complementary in vitro transcribed RNA segments each labeled with a specific fluorophore. The linear order of these differently colored RNA segments creates a unique code for each gene of interest.
  
  Capture and Reporter probes are synthesized in 96-well plates using a semi-automated process.
  
  A different version of this platform with Gene Symbol in the ID column is assigned accession number GPL17804.
manufacture_protocol: See manufacturer's website http://www.nanostring.com