title: PGA/MGH mouse v1.1 oligonucleotide array
status: Public on Jan 29 2010
contact.city: Dublin
contact.first: Stephen
contact.middle: Francis
contact.last: Madden
contact.email: stephen.madden@dcu.ie
contact.address: Glasnevin
contact.department: National Institute for Cellular Biotechnology
contact.country: Ireland
contact.institute: Dublin City University
contact.phone: 0035317006287
organism: Mus musculus
manufacture_protocol: The PGA mouse v1.1 oligonucleotide probe sets (70-mers) were printed using an OmniGrid 100 printer (GeneMachines) on CodeLink-activated slides (Amersham). Oligos were printed in 150 mM sodium phosphate pH 8.5 with 0.0005% N-lauroyl sarcosine. Oligos were coupled to the activated slides by allowing the aminolinker on the oligos to react overnight in a humidity chamber. Unreacted groups on the slide surface were blocked with 150 mM glycine, 0.1 M tris, 0.1% N-lauroyl sarcosine, pH 9.