title: Agriculture and Agri-Food Canada B. napus Westar 15K v1.0
status: Public on Feb 01 2009
contact.city: Saskatoon
contact.first: Dwayne
contact.last: Hegedus
contact.email: dwayne.hegedus@agr.gc.ca
contact.state: SK
contact.address: 107 Science Place
contact.country: Canada
contact.institute: Agriculture and Agri-Food Canada
contact.phone: (306) 385-9427
organism: Brassica napus
description: 2 spots printed for each probe
manufacture_protocol: The B. napus 50-mer oligo array was developed by Agriculture and Agri-Food Canada using the collection of approximately 150,000 3′ and 5′ B. napus ESTs derived from cDNA libraries representing a range of vegetative tissues of the homozygous line DH12075. A custom unigene set of sequences identified from the collection was used for oligo design with the aim of developing a resource with the potential to detect differential expression between members of closely related multi-gene families within the polyploid B. napus genome. A full description of the development of the set of 15,000 50-mer oligos will be published separately (Sharpe et al., in preparation). The oligos were synthesized by Illumina (San Diego) and printed onto epoxide slides (Corning Inc. Lovell, MA, USA) using a 48-pin GeneMachines arrayer at the University of Alberta Microarray Facility (Edmonton, AB, Canada) Microarray Facility. Each one of the 48 sub-arrays were designed with 26 columns × 25 rows for a total capacity of 31,200 features. Each of the 15,000 oligos were replicate spotted within each sub-array. The Stratagene Alien oligo set (La Jolla, CA, USA) and phosphate buffer blanks were added as controls.