title: NimbleGen Bordetella 4x72K custom design expression array (genes)
status: Public on Nov 21 2014
contact.city: Utrecht
contact.first: Aldert
contact.last: Zomer
contact.email: A.L.Zomer@uu.nl
contact.address: Yalelaan 1
contact.department: Department of Infectious Diseases and Immunology,Faculty of Veterinary Medicine
contact.country: Netherlands
contact.institute: Utrecht University
organism: Bordetella bronchiseptica
  Bordetella pertussis
description: The NimbleGen array contained 1-8 probes for all coding sequences (CDS) with an average coverage of 7.5 probes per CDS and pseudogene, 15 bp overlapping tiling probes covering both strands of the intergenic regions, and 7,304 random probes with a similar length distribution and GC content as the experimental probes. The array design was based on the genome sequence of B. pertussis Tohama I, supplemented with additional B. bronchiseptica CDS and intergenic sequences that are present in the B. pertussis strains used in this study.
  110228_Bpertussis_TohamaI_HB_exp.ndf
  110228_Bpertussis_TohamaI_HB_exp.ngd
manufacture_protocol: High-density DNA array prepared with Maskless Array Synthesizer (MAS) technology. See manufacturer's website at http://www.nimblegen.com/