title: UniHH/Scholten_zea mays_heterosis 3k-array
status: Public on Aug 28 2009
contact.city: Hamburg
contact.first: Stefan
contact.last: Scholten
contact.email: stefanscholten@botanik.uni-hamburg.de
contact.address: Ohnhorststrasse 18
contact.department: Biocenter Klein Flottbek
contact.country: Germany
contact.institute: University of Hamburg
contact.phone: 0049 40 42816329
organism: Zea mays
manufacture_protocol: Individual cDNA inserts of diverse cDNA libraries were amplified with insert flanking primers. The PCR products were visualized on 1% agarose gels to test the quality and quantity of amplification, followed by purification with Multiscreen PCR plates (Millipore). The clean PCR products were reformatted in 384 well plates, dried and resuspended in 15 µl spotting buffer (3 x SSC, 1,5 M betain). Each PCR product was printed twice onto poly-L-lysine (Sigma) coated glass slides with a Microgrid II robot (BioRobotics). Slides were blocked with succinic anhydride and denatured in boiling water.