title: Agilent-033677 Biomphalaria glabrata Transcriptome v2_13Apr11 030447
status: Public on Jul 23 2015
contact.city: Albuquerque
contact.first: Si-Ming
contact.last: Zhang
contact.state: NM
contact.address: Castetter Hall
contact.department: Biology
contact.country: USA
contact.institute: University of New Mexico
organism: Biomphalaria glabrata
description: The ID column represents the Agilent Feature Extraction feature number.
manufacture_protocol: see manufacturer's web site at http://www.agilent.com/
  
  Publicly available cDNA sequences (Genbank, dbEST, SRA; 2010) for Biomphalaria glabrata were assembled using Mira. Transcripts were analyzed and as needed reverse complemented for longest continuous ORF. A total  of 30593 predicted transcripts, supplemented with 15 mitochondrial gene sequences from Biomphalaria glabrata, 26 (12 nuclear + 14 mitochondrial) gene sequences from Schistosoma mansoni and  12 (9 nuclear + 3 mitochondrial) gene sequences from Echinostoma paraensei) were used as targets for e-array (Agilent) design of unique 60-mer probe sequences. Duplicate probes were arranged in 8 x 60k custom microarray format with Agilent 60-mer SurePrint technology.  Design name Biomphalaria glabrata Transcriptome v2_13Apr11. Created date 13 Apr 2011. Control Grid: IS-62976-8-V2_60kby8_GX_EQC_201000210 . Feature layout: randomized. Design ID: 033677