title: OSUrbt Oncorhynchus mykiss 1.6K v2.0 update 07.05.07
status: Public on Jul 13 2007
contact.city: Logan
contact.first: Abby
contact.middle: D
contact.last: Benninghoff
contact.email: abby.benninghoff@usu.edu
contact.state: UT
contact.address: 4815 Old Main Hill
contact.department: Animal, Dairy and Veterinary Sciences
contact.country: USA
contact.institute: Utah State University
organism: Oncorhynchus mykiss
description: 4 X 4 pin arrangement Microspot 2500 pins (quill design)
manufacture_protocol: Oligonucleotides were resuspended in spotting buffer (3X SSC and 1.5 M betaine) at 25 uM final concentration and were printed onto Corning UltraGap slides (Acton, MA) using a Biorobotics Microgrid II arrayer (Genomic Solutions, Ann Arbor, MI) with 16 pins. The trout 70-mers were each printed in duplicate and a set of ten SpotReport Alien Oligos (Stratagene, La Jolla, CA) were printed 16 times (once per block) on the array. After printing, slides were desiccated for 24-48 hrs and cross-linked with UV Stratalinker (Stratagene) at 600 mJ.