title: Arizona Gallus gallus 20.7K Oligo Array v1.0
status: Public on Mar 11 2015
contact.city: Saint Paul
contact.first: Sunantha
contact.last: Kosonsiriluk
contact.email: koson003@umn.edu
contact.state: Minnesota
contact.address: 495 AnSci/VetMed 1988 Fitch Ave
contact.department: Animal Science
contact.country: USA
contact.institute: University of Minnesota
organism: Gallus gallus
manufacture_protocol: The chicken oligos were designed by Carre et al. (2007) using gene predictions from Ensembl, TIGR, full-length cDNAs from the BBSRC UMIST ChickEST database, and chicken bursal EST sequences from Jean-Marie Buerstedde's DT40 database. Sequences from these resources were aligned and 70mer oligos designed against regions common to more than one aligned sequence. Oligos were then designed using OligoArray2.0.
  The chicken oligo set [Gallus gallus (chicken) Roslin/ARK CoRe Array V1.0] was manufactured by Operon Technologies, Inc.. The chicken oligo set (Catalog # 851102) contains 20,460 long oligo probes (300 pmol) arrayed in 54 384-well plates. Four wells in each of the 54 384-well plates contained the Operon Production Tracking Oligo opHsV04NC000001, which is a randomly-generated oligo sequence with a length of 30 bases.
  The 20.7K chicken oligo array contains 20,676 features that include 20,460 70mer oligos and 216 control spots of the Operon Production Tracking Oligo opHsV04NC000001.