title: TAGC Human 1075 Clones
status: Public on Nov 18 2006
contact.city: Marseille
contact.first: Pascal
contact.last: FINETTI
contact.email: finettip@ipc.unicancer.fnclcc.fr
contact.state: BdR
contact.address: 232 Bd Ste Marguerite
contact.department: Centre de cancérologie de Marseille
contact.country: France
contact.institute: Institut Paoli-Calmettes
contact.phone: (33)+4 91 22 33 04
organism: Homo sapiens
manufacture_protocol: We used DNA microarrays to compare the mRNA expression profiles of ~1.000 selected genes between primary, HS, and HR tumors in the LuCaP 23.1 xenograft model of prostate carcinoma. RNA extracts were pooled according to the three distinct stages of tumor progression in the LuCaP 23.1 model (4 samples / tumor stage). DNA microarray hybridizations were done as previously described (14, 15) on home-made nylon DNA microarrays (TAGC, Marseille-Luminy, France), which contained spotted PCR products from 945 human cDNA clones. Most genes were selected for a proven or putative implication in cancer and/or in immune reactions (the list is available on the web site: http:/tagc.univ-mrs.fr/pub/Cancer/).