title: AsalChip2 (NRC-IBS Aeromonas salmonicida A449)
status: Public on Apr 29 2008
contact.city: Ottawa
contact.first: John
contact.middle: H
contact.last: Nash
contact.email: John.Nash@gmail.com
contact.state: Ontario
contact.address: 100 Sussex Drive
contact.department: Institute for Biological Sciences
contact.country: Canada
contact.institute: National Research Council
organism: Aeromonas salmonicida subsp. salmonicida A449
description: PCR primers were designed for 4293 ORFs from the completed genomic sequence of Aeromonas salmonicida A449 which comprises 4413 ORFs on one chromosome and two large plasmids. Good amplicons of length greater than 160 nt were obtained for 4237 ORFs representing >95% coverage of ORFs. All amplicons are spotted in duplicate on the microarray and sheared total genomic DNA from A. salmonicida and salmon DNA were spotted in the corners of each subarray as controls.
manufacture_protocol: DNA microarrayer (Bio-Rad) equipped with a 48-pin print-heads that uses "quill" pin technology (TeleChem / ArrayIt).