title: Hypertension Microarray Platform
status: Public on Jul 31 2007
contact.city: Phildelphia
contact.first: Rishi
contact.last: Khan
contact.email: rishi@mail.dbi.tju.edu
contact.state: PA
contact.address: 1020 Locust Street Room 381
contact.department: Pathology
contact.country: USA
contact.web_link: http://www.dbi.tju.edu
contact.institute: Thomas Jefferson University
organism: Rattus norvegicus
manufacture_protocol: Microarray Manufacture. Microarrays were fabricated using a rat clone set (GF200; ResGen Huntsville, AL, and Invtrogen, Carlsbad, CA) for cDNA microarrays consisting of approximately 8,800 sequence-verified non-redundant (as of Unigene build 150). The cDNA clones from all rat cDNA targets were prepared from freshly grown overnight bacterial cultures by PCR amplification using GF200 primers (Invitrogen, Carlsbad, California). PCR products were purified and verified by agarose gel electrophoresis, and the yield was determined spectrophotometrically (NanoDrop Inc., Wilmington, DE). cDNAs were mixed with an equal volume of DMSO (10-70 ng/ul) and printed onto Corning cDNA slides (Corning, Corning, NY) using a MicroGrid II arrayer (Genomic Solutions, Ann Arbor, MI). Microarrays were air dried for 30 min and cross-linked by UV irradiation. We printed arrays of 18,240 spots representing ~9000 clones in adjacent duplicate spots. The arrays used in the present study had approximately 5,300 annotated genes based on the Entrez Gene database.