title: D. melanogaster detoxification array
status: Public on Mar 20 2007
contact.city: Parkville
contact.first: Lee
contact.last: Willoughby
contact.email: lwil@unimelb.edu.au
contact.state: Victoria
contact.address: 30 Flemington Road
contact.department: Genetics
contact.country: Australia
contact.institute: University of Melbourne
organism: Drosophila melanogaster
description: The Drosophila melanogaster "detox" microarray contained the cytochrome P450, glutathione-S-transferase and esterase gene families and also incorporated both individual control genes and control dilutions, to account for the large number of "detox" genes that could have potentially been differentially regulated in response to different treatments.
manufacture_protocol: Fragments of the Drosophila melanogaster P450, GST, esterase and additional control genes were amplified by PCR and cloned into plasmid vectors.  DNA fragments for printing were amplified from the plasmid inserts using universal primers, which were then purified before spotting.
  Three additional series of control spots were included.
    (A) Lucidea Universal Scorecard (Amersham) was included.
    (B) Digested - Drosophila melanogaster LD EST library (BDGP) was excised and digested with BglII and HindIII.  This was then serially diluted to generate a dilution series.
    (C) Pooled - PCR products from the ribosomal control genes were pooled and this was then serially diluted to generate a dilution series.
  Each glass slide contained four microarrays, allowing dye swap hybridisations to be performed in the same hybridisation chamber.  Each unique feature (not the control dilution series) was duplicated four times in a four printing block arrangement.  The microarrays were printed on Corning GAPS II slides (Corning), using a Virtek SDDC2 microarrayer (Virtek Vision International Inc.).