title: mKIAA cDNA microarray ver2.0
status: Public on Jun 14 2005
contact.city: Kisarazu
contact.first: Kazuhide
contact.last: Imai
contact.email: imai@kazusa.or.jp
contact.state: Chiba
contact.address: 2-6-7 Kazusa-Kamatari
contact.country: Japan
contact.web_link: http://www.kazusa.or.jp/create
contact.institute: Chiba Industry Advancement Center
contact.phone: +81-438-52-3988
organism: Mus musculus
manufacture_protocol: Description of mKIAA cDNA microarray ver2.0: Description of mKIAA cDNA microarray: 1707 duplicated mKIAA (1612) and mFLJ cDNA (95) clones and several controls  (e.g., house keeping genes, negative controls, artificial external control plasmids) are spotted on  nylon membrane microarray (18´55 mm). The plasmid DNA was amplified using the TempliPhi DNA amplification kit (Amersham, Piscataway, NJ) in twin.tec PCR plate 384 (eppendorf, Hamburg, Germany). Ampliphied DNA mixture was dried and then disolved in 80% formamide, 20mM EDTA (see detail of  the method in Yamakawa, H., et.al., 2004, DNA Res., 11, 353-360). The mixture were spotted onto a nylon membrane (Biodyne Plus; Pall, East Hills, NY) attached to a slide glass using GeneTac RA1 microarrayer (Genomic Solutions, Ann Arbor, MI).