title: Mycroarray-Saccharomyces_cerevisiae-30K
status: Public on Aug 23 2013
contact.city: Seoul
contact.first: Hye Yun
contact.last: Moon
contact.email: moon790322@naver.com
contact.state: Seoul
contact.address: , 221 Heukseok-dong, Dongjak-gu
contact.department: Department of Life Science
contact.country: South Korea
contact.institute: Chung-Ang university
contact.phone: +82-2-821-5863
organism: Saccharomyces cerevisiae
description: Saccharomyces Cerevisiae Oligonucleotide 30K Microarrays
manufacture_protocol: We directly synthesize them on a microscope slide. Our synthesis is using the robust phosphoramidite chemistry refined since the 1970's for commercial oligonucleotide synthesis. In standard oligonucleotide synthesis, the terminal acid-labile protective group of a growing oligomer is removed by a treatment with a strong acid.
  The only modification we have introduced in our process is the usage of a photo-generated acid. At a given synthesis cycle, each spot that needs to be deprotected is exposed to light in the presence of a photogenerated acid precursor, leading to local acid production and cleavage of the terminal protective group. Only the deprotected spots will be able to react with a new monomer. By so doing, we can synthesize tens of thousands oligonucleotides in parallel on the same microarray substrate.
  The advantage of using the phosphoramidite chemistry is that we can synthesize oligonucleotides at a reduced cost compared to other chemistries while maintaining our stepwise yield well above 99%.
  To expose the oligonucleotide spots to light during the deprotection step of the synthesis cycle, we do not use any physical mask but instead we use a digital micromirror device (DMD) made of several hundred of thousands individually addressable mirrors. At each deprotection step, a computer simply generates an image to be projected on the microarray surface. Using this digital photolithography approach, we can synthesize any custom microarray with no upfront design cost or minimal order size.
  We synthesize our microarrays on high quality microscope slides. For uniform synthesis and hybridization and enhanced scanner image quality, we use only low-background glass slides polished to atomic flatness (±20 angstroms). Their precise physical dimension (25 ± 0.2 mm x 76 ± 0.3 mm x 0.940 mm ± 0.025 mm) is compatible with all major brands of slide microarray scanners.