title: MacArary Karyo 4000
status: Public on Jun 11 2009
contact.city: New York
contact.first: Jiun
contact.last: Kang
contact.email: ju2497@gmail.com
contact.state: NY
contact.address: 1130 St. Nicholas Avenue
contact.department: Pathology
contact.country: USA
contact.institute: Columbia University
contact.phone: 917-575-0205
organism: Homo sapiens
description: The array was based on NCBI build 36.2, UCSC hg18 of the human genome and used in this study consists of 4,044 human Bac-terial Artificial Chromosomes (BACs), which were spaced approximately 1.0 Mb on average across the entire genome
manufacture_protocol: The clones were prepared by conventional alkaline lysis
  method to obtain BAC DNA. The DNA was sonicated to generate fragments of ~3 kb and then was mixed with 50% dimethyl sulfoxide spotting buffer. The arrays were manufactured using a Molecular Dynamics (Sunnyvale, CA) Generation III spotter in an eight-pin format. BAC clones were represented on an array as duplicated spots and each array was prescanned using an Axon scanner for proper spot morphology (Axon InstrumentsdMolecular Devices, Sunnyvale, CA). The array used in this study consists of 4,046 human BACs, which were spaced ~1 Mb across the whole genome.