title: Custom Yeast Genomic 70-mer Oligonucleotide Microarray
status: Public on Feb 28 2005
contact.city: Urbana
contact.first: Kurt
contact.middle: E
contact.last: Kwast
contact.email: kwast@uiuc.edu
contact.state: IL
contact.address:  407 S. Goodwin Ave.
contact.department:  Molecular & Integrative Physiology
contact.country: USA
contact.institute: University of Illinois at Urbana-Champaign
contact.phone: 217-244-3122
organism: Saccharomyces cerevisiae
description: The custom microarrays consisted of Operon’s Yeast Genomic 70-mer Oligonucleotide Set (Qiagen, Valencia, CA, v. 1.1) spotted in duplicate at a concentration of 20 mM in 150 mM sodium phosphate (pH 8.5), Arabidopsis oligonucleotide spike controls (Stratagene SpotReport, La Jolla, CA) spotted in quadruplicate, and 10 human and 10 yeast oligonucleotide negative controls spotted in duplicate.  The oligonucleotides were printed on Codelink slides (Amersham, Piscataway, NJ) by Microarrays, Inc. (Nashville, TN).  Post-print processing was conducted according to the manufacturer’s recommendations.