title: BC013
status: Public on Aug 27 2013
contact.city: Boston
contact.first: Jennifer
contact.middle: L
contact.last: Bowen
contact.email: jennifer.bowen@umb.edu
contact.state: MA
contact.address: 100 Morrissey Blvd
contact.department: Biology
contact.country: USA
contact.institute: University of Massachusetts Boston
organism: synthetic construct
manufacture_protocol: 1.The probe oligonucleotides are printed onto Corning Ultragaps or Erie Scientific Epoxy Silane slides using a DeRisi style arrayer (DiRisi et al., 1997) and pins from Parallel Synthesis.
  2.The 70-mer oligo probes (Operon Technologies) were adjusted to a concentration of 0.05 mg ml-1 in 50%  DMSO and were spotted on CMT-GAPS amino silane-coated  glass slides (Corning, Corning, NY).
  3. After printing, the slides were baked at 80°C for 3 h and stored in the dark at room temperature under slight vacuum in a dessicator.