title: RIKILT mouse oligo 10K array
status: Public on Apr 15 2006
contact.city: Wageningen
contact.first: ping
contact.last: wang
contact.email: ping.wang@hb.unimaas.nl
contact.address: bornsesteeg 45
contact.country: Netherlands
contact.institute: RIKILT / Maastricht University
organism: Mus musculus
manufacture_protocol: The 10K MWG mouse oligoset (10K_A, MWG-Biotech AG, Ebersberg, Germany) together with additional 50-mer oligo probes genes encoding secretory proteins and proteins involved in energy metabolism, were in-house printed on GAP II coated slides (Corning), using the Microgrid II arrayer (BioRobotics Ltd, Cambridge, UK). Majority of the probes of the additional oligoset of the extra set were also from MWG. The rest (8 genes) were designed through OligoFaktory server (http://ueg.ulb.ac.be/oligofaktory/index.jsp) and synthesized by Isogen (Maarssen, the Netherlands) and Biolegio (Nijmegen, the Netherlands). After printing, microarrays were allowed to dry at room temperature. The oligo arrays were immobilized by UV cross-linking (120 MJ of UV energy). The microarray slides were then prewashed in the following order to reduce background; 20 minutes at 55C with 2x SSC/0.2% SDS, 5 minutes at room temperature (RT) with 0.2x SSC and 3 minutes at RT with demineralized water. Slides were dried by 2 minutes of centrifugation (2000 rpm).