title: Utz Lab ACA Array version 3
status: Public on Feb 26 2025
contact.city: Stanford
contact.first: Allan
contact.last: Feng
contact.email: afeng1@stanford.edu
contact.state: CA
contact.address: 269 Campus Dr
contact.department: Imm/Rheum
contact.country: USA
contact.institute: Stanford School of Medicine
contact.phone: 2055678956
organism: Homo sapiens
description: Homo sapiens, E.Coli, HEK293 cells
manufacture_protocol: We conjugated recombinant protein antigens to uniquely barcoded magnetic  beads (MagPlex® Microspheres). 8 ug of each antigen or control antibody was diluted in phosphate buffered saline (PBS) and transferred to 96-well plates. Diluted antigens and control antibodies were conjugated to 1 million carboxylated magnetic beads per ID.