title: LC_MRA-1002_miRMouse_15 (miRNA ID version)
status: Public on Oct 10 2012
contact.city: Changchun
contact.first: Pingsen
contact.last: Zhao
contact.email: zhaopingsen@hotmail.com
contact.state: Jilin
contact.address: No. 666 Liuying Xilu
contact.department: The research centre for canine diseases
contact.country: China
contact.institute: Institute of Military Veterinary Academy of Military Medical Sciences
organism: Mus musculus
description: Based on Sanger miRBase Release 15.
manufacture_protocol: Arrays are made based µParaflo microfluidic technology (Atactic Technologies). On the µParaflo microfluidic chip, each detection probe consists of a chemically modified nucleotide coding segment complementary to target microRNA (from miRBase, http://microrna.sanger.ac.uk/sequences/) or other RNA (control or customer defined sequences) and a spacer segment of polyethylene glycol to extend the coding segment away from the substrate. The detection probes are made by in situ synthesis using PGR (photogenerated reagent) chemistry.