title: Agilent-018073 Custom Brassica 60-mer two-color microarray
status: Public on May 03 2009
contact.city: Norwich
contact.first: Andreas
contact.last: Magusin
contact.email: andreas.magusin@jic.ac.uk
contact.address: Norwich Research Park
contact.department: Crop Genetics
contact.country: United Kingdom
contact.institute: John Innes Centre
organism: Brassica
manufacture_protocol: The assembled Brassica sequences (94,558 instances including those represented in both orientations) were submitted to Agilent Technologies’ eArray web portal for gene expression probe design. Probes with a base composition score greater than or equal to 3 were omitted from the final design; resulting in a total of 91,854 unique probes (including 6,989 derived from oppositely oriented pairs of sequences) that were included in the microarray design, of which 10,466 were predicted to have cross-hybridization potential.
  11,893 probes were randomly selected to be represented in duplicate in the final design, which also included Agilent Technologies’ standard panel of quality control and spike-in probes.
  Microarrays were manufactured using Agilent Technologies’ SurePrint Technology in the 2x104k format (two arrays containing ~104,000 probes on a single 1” x 3” glass slide).