title: Schistosoma japonicum 3.1K
status: Public on Mar 10 2009
contact.city: shanghai
contact.first: yuan
contact.last: chunxiu
contact.email: yuanchx@online.sh.cn
contact.country: China
contact.institute: shanghai research institute of domestic animal parasitolgy
contact.phone: 86-21-54082675
organism: Schistosoma japonicum
description: Spotted Schistosoma japonicum differentially expressed gene array on glass.this set includes 3118 gene clone ,2 tubulin control and 16 blank. These gene clone are from subtracted cDNA library. The PCR products of cDNA were amplified by use of the polymerase chain reaction (PCR) and then purified with isopropanol precipitation. All PCR products were examined by agarose gel electrophoresis to ensure the quality and the identity of the amplified clones as expected. Then the amplified PCR products were dissolved in a buffer containing 3X SSC solution. The solution with amplified PCR products were spotted onto silylated slides(Biostar, Shanghai, China) with a Omnigrid Arrayer(Gene Machine,CA) fitted with 16 Major Precision split pins.Glass slides with spotted cDNA was then hydrated for 2 hours in 70% humidity, dried for 0.5 hour at room temperature, UV cross-linked (65mj/cm). They were further processed at room temperature by soaking in 0.2% sodium dodecyl sulfate (SDS) for 10 min, distilled H2O for 10 min, and 0.2% sodium borohydride (NaBH4) for 10 min. The slides were dried again and ready for use.