title: RayBio Homo sapiens 0.1K apoptosis array
status: Public on Dec 31 2010
contact.city: Newark
contact.first: David
contact.middle: M.
contact.last: Lukac
contact.email: lukacdm@njms.rutgers.edu
contact.state: NJ
contact.address: 225 Warren St.; ICPH E350C; PO Box 1709
contact.department: Microbiology, Biochemistry and Molecular Genetics
contact.country: USA
contact.institute: Rutgers Univ./NJ Medical School
contact.phone: 973 972-4868
organism: Homo sapiens
description: Principle of the Assay
  With RayBio® Human Apoptosis Antibody Array kit, researchers can now simultaneously detect the relative level of 43 apoptosis related proteins in cell lysates. By monitoring the changes in protein levels in different experimental model systems, researchers can study pathway activation without spending excess time and effort in performing immunoprecipitations and/or Western Blotting.
  With RayBio® Human Apoptosis Antibody Array kit, each of the capture antibodies is printed on the membranes, and then treated or untreated cell lysate is added into antibody array membranes. After extensive washing, the membranes are incubated with a cocktail of biotin-conjugated anti-apoptotic protein antibodies. After incubation with	HRP-Streptavidin,	 the signals	are	visualized	by chemiluminescence
manufacture_protocol: see manufacturer's website