title: Campylobacter jejuni 6.5K whole-ORF array v1.0
status: Public on Sep 10 2008
contact.city: East Lansing
contact.first: Linda
contact.middle: S.
contact.last: Mansfield
contact.email: mansfie4@cvm.msu.edu
contact.state: MI
contact.address: 181 Food Safety Bldg.
contact.department: Microbiology and Molecular Genetics
contact.country: USA
contact.institute: Michigan State University
contact.phone: 517-884-2027
organism: Campylobacter jejuni
manufacture_protocol: 1.) Primers were designed at Wayne State University by Jodi R. Parrish, furnished to Michigan State University and are described in “High-Throughput Cloning of Campylobacter jejuni ORFs by in Vivo Recombination in Escherichia coli”, Journal of Proteome Research, Parrish et al., 2004 3(3):582-6.
  2.) PCR products were amplified from genomic DNA from Campylobacter jejuni 11168 or plasmid DNA obtained from C. jejuni 81-176.  Products were ethanol precipitated, resuspended in 3X SSC, 0.005% SDS print buffer, and run on gels for quality control.
  3.) 16 pin replicate arrays were printed on Corning UltraGAPS slides using a GenMachines Omnigrid spotting robot and Telechem Chipmaker 3 pins.
  4.) Each slide contains every spot in triplicate.