title: MAICH Horticultural genetics-Olea europaea-Kalamon v.1
status: Public on Nov 20 2012
contact.city: Chania
contact.first: Maria
contact.middle: E.
contact.last: Manioudaki
contact.email: manioudaki@maich.gr
contact.address: Makedonias 1
contact.department: Horticultural Genetics and Biotechnology
contact.country: Greece
contact.institute: MAICH
organism: Olea europaea
manufacture_protocol: The unique cDNAs were PCR-amplified using Taq polymerase (Minotech, Heraklion, Greece) with the primers (pDNR-F, 2: 5'-CCGCATAACTTCGTATAGC–3' and pDNR-R, 2: 5'-CATGTTCACTTACCTACTGG-3') positioned by the polylinker site. The PCR products were purified using Nucleofast 96 columns (Macherey-Nagel, Duren, Germany). Approximately 3 μg of purified PCR product was placed in 384-well printing plates (Genetix, Hampshire, UK), dried in room temperature and resuspended in printing buffer containing 450 mM NaCl, 45 mM phosphate pH 7.0, 5% [v/v] formamide and 0.01% [v/v] maltoside at a final concentration of 200 μg/μl. Printing was performed in triplicates on home-made aminosilane slides using a Packard SpotArray24 spotter with four print-tips.