title: Mm-FDA-OperonV2
status: Public on Mar 11 2006
contact.city: Bethesda
contact.first: Amy 
contact.middle: Xiaoyun
contact.last: Yang
contact.email: amy.yang@fda.hhs.gov
contact.state: MD
contact.address: 8800 Rockville Pike
contact.department: Division of Celular and Gene Therapies
contact.country: USA
contact.web_link: http://@fda.hhs.gov
contact.institute: CBER, FDA
contact.phone: 301-827-9770
organism: Mus musculus
manufacture_protocol: The mouse genome oligonucleotide probe set contains 16,896 optimized 70mers (plus controls) obtained from Operon (http://www.operon.com/). The oligonucleotide probes were purchased in 384-well polypropylene plates (Genetix, 384 micro array) and dissolved in 4XSSC print buffer at a concentration of 20-30 uM. The microarray is composed of 32 blocks and one spot is printed per probe per slide. The spot size is 100 micron and spot distance is 185 microns (center-to-center). The microarrays were printed on Gold Seal micro slides (Cat #3010) coated with poly-L-lysine and post-processed according to Operon's protocol.