title: Zebrafish custom Agilent temperature_cadmium microarray
status: Public on Aug 27 2010
contact.city: Wilrijk
contact.first: Lucia
contact.last: Vergauwen
contact.email: lucia.vergauwen@uantwerpen.be
contact.address: Universiteitsplein 1
contact.department: Veterinary Sciences
contact.country: Belgium
contact.institute: University of Antwerp
contact.phone: 003232652791
organism: Danio rerio
description: A custom cadmium and temperature specific 15k microarray was designed. For this purpose 16 Agilent zebrafish 44k full genome microarray (version V2, AMADID 019161, containing 43,604 unique probes) hybridizations were performed on a subsample of the test samples from a temperature experiment and a cadmium exposure (performed with the intention of comparing temperature and cadmium responses in zebrafish). Probes that were differentially expressed under these conditions were included in the custom design and supplemented with GO-annotated probes from the full genome microarray probe set.
manufacture_protocol: 60-mer oligonucleotides printed by Agilent