title: US Army ERDC Fathead minnow brain tissue cDNA 4K Microarray
status: Public on Jul 30 2010
contact.city: Vicksburg
contact.first: Kurt
contact.middle: A
contact.last: Gust
contact.email: kurt.a.gust@usace.army.mil
contact.state: MS
contact.address: 3909 Halls Ferry Rd.
contact.department: Environmental Laboratory
contact.country: USA
contact.institute: US Army ERDC
contact.phone: 601-634-3593
organism: Pimephales promelas
manufacture_protocol: Microarrays were constructed by spotting the fathead minnow cDNA library, ArrayControl™ Spot cDNAs (Ambion), and printing buffer blanks onto Ultra GAPS-coated microarray slides (Corning Inc., Corning, NY).  Spotted cDNAs were immobilized on the chip surface by applying 300 mJ of UV radiation in a Stratagene™ UV Stratalinker 2400 (Agilent Technologies, Waldbronn, Germany).