title: Custom Genome PCR Tiling Array of the Igf2r Imprinted Gene Cluster
status: Public on Jul 01 2007
contact.city: Vienna
contact.first: Denise
contact.middle: P.
contact.last: Barlow
contact.email: denise.barlow@univie.ac.at
contact.address: Dr. Bohrgasse 9/4
contact.department: Austrian Academy of Sciences
contact.country: Austria
contact.institute: Center of Molecular Medicine (CeMM)
organism: Mus musculus
manufacture_protocol: The tiling array contained only single copy sequence (exons, introns and intergenic regions) from the Igf2r imprinted gene cluster on mouse chromosome 17. Repeat elements were excluded by RepeatMasker (www.repeatmasker.org). PCR primers were designed with Primer3 (http://frodo.wi.mit.edu/cgi-bin/primer3/primer3_www.cgi) to amplify all unique single copy mouse sequence. The PCR products were overlapping with an average size of 430bp. The manufacture protocol was adapted from "Diehl, F. et al. (2001). Manufacturing DNA microarrays of high spot homogeneity and reduced
  background signal. Nucleic Acids Research: 29(7), e38" with the exception of using 1-methyl 2-pyrrolidone instead of 1,2-dichlorethane. Each PCR product was spotted in 8 positions on Nexterion slides (Schott) with a Promedia Associates Inc. printer (Model PA-MP2000) using SMP3 printing tips (Telechem).