title: Center for Functional Analysis of Human Genome Human 21K
status: Public on Mar 26 2008
contact.city: Daejon
contact.first: Seon-Young
contact.last: Kim
contact.email: kimsy@kribb.re.kr
contact.address: 52 Eoeun-dong, Yuseong-gu
contact.department: Genome Research Center
contact.country: South Korea
contact.institute: Korea Research Institute of Bioscience and Biotechnology
contact.phone: 82-42-879-8116
organism: Homo sapiens
manufacture_protocol: cDNA microarrays were fabricated in our core facility using a robotic arrayer (PixSYS 5500, Cartesian; Ann Arbor, MI). The 21K microarray contains 21,168 genes from KUGI unigene library. All the clones were verified by sequencing in our facility. The cDNA inserts were amplified by PCR, and after purification, spotted onto silylated glass slides (CSS-100; Telechem, Sunnyvale, CA). Each microarray slide contained control genes to normalize the effect of different fluorescent dyes.