title: DKFZ/Operon Human Oligo Set v4.0
status: Public on Jan 12 2008
contact.city: Mannheim
contact.first: Carsten
contact.last: Sticht
contact.address: Theodor-Kutzer-Ufer
contact.department: ZMF
contact.country: Germany
contact.institute: University Heidelberg
organism: Homo sapiens
description: Spotted long oligonucleotide array produced using the Operon Human Genome Oligo Set Version 4.0.
  Keywords = human
  Keywords = long oligonucleotide
  Keywords = oligo
  Keywords = 70mer
  Keywords = Operon
manufacture_protocol: 70mer oligonucleotides were diluted at 40 µM in FBNC buffer (G. Wrobel et al., Nucleic Acids Res 31 e67, 2003) and spotted as array duplicates using epoxy-silane coated glass slides (Schott Nexterion) and stainless steel split pins (SMP3, Telechem) at 20°C and 40% rH. Slides were dried (60' @ 60°C) and DNA was UV-crosslinked with 240 mJ/cm2 @ 254 nm. Slides were stored, sealed airtight and dry, at 4°C.