title: Genomessage Homo sapiens 0.7k
status: Public on Oct 10 2007
contact.city: Kawagoe-shi, Saitama
contact.first: Tsutomu
contact.last: Takeuchi
contact.address: 1981 Tsujido-machi, Kamoda
contact.department: Department of Internal Medicine
contact.country: Japan
contact.institute: Saitama Medical University
organism: Homo sapiens
manufacture_protocol: For most of the genes, each cDNA was designed approximately 500-600 bp and within approximately 1 kb region from the 3’-poly(A) tail and all cDNAs for microarray probe were cloned into the pGEM vector (Promega, Madison, WI).  We also selected and cloned external control genes (approximately 0.5-1 kb) into the pGEM vector to establish the dynamic range of the microarray.  Renilla luciferase (RL), baculovirus envelope gp64 (GP), and lambda phage DNA (LD).  All clones for capture probe on microarray were sequenced and validated by comparison with the GenBank sequence.   The aminosilane surfaces of SuperAmine glass slides (TeleChem International, Sunnyvale, CA) were stamped with quadruply spots of cDNA probe corresponding to each of the capture probe genes with a Geneqs 2000 arrayer (KakenGenex, Matsudo, Japan).  The average spot size was 150 mm and separated each other with a distance (500 mm).