title: CombiMatrix Bovine 90K Array TM 1.0
status: Public on Nov 13 2011
contact.city: Lodi
contact.first: Marcello
contact.last: Del Corvo
contact.email: marcello.delcorvo@tecnoparco.org
contact.address: via Einstein - Loc. Cascina Codazza
contact.department: Bioinformatics
contact.country: Italy
contact.institute: CNR-Ibba
contact.phone: 003903714662200
organism: Bos taurus
description: The Bovine array includes 43,822 probes designed using the program OligoArray 2.1 (Rouillard et al. 2003): 20,859 specific oligos (35-40mer) were designed on Ensembl release 50, 19,230 were designed on Bovine Unigene and 3,550 on Bovine EST database (Jul 2008), while 183 oligonucleotides sequences, provided by the manufacturer, were used as quality and negative controls. The 90K BovineArrayTM 1.0 was designed with two replicates of each probe randomly distributed across the array to allow measurement of the variability within the array. All the procedures provided were performed as indicated by CombiMatrix protocols available at the CombiMatrix website (www.combimatrix.com)
manufacture_protocol: Probes are directly synthesized on a 90k Combimatrix array by an in situ oligo synthesis system developed by CombiMatrix Corporation (Mukilteo, USA) using the CombiMatrix CustomArray synthesizer. Standard synthesis protocol provided by CombiMatrix was used