title: PIQOR (TM) Custom_CTID88 Microarray, mouse (88)
status: Public on Dec 31 2007
contact.city: Bergisch Gladbach
contact.first: Silvia
contact.last: Rueberg
contact.email: silvia@miltenyibiotec.de
contact.address: Friedrich-Ebert-Str. 68
contact.department: Genomic Services
contact.country: Germany
contact.institute: Miltenyi Biotec GmbH
organism: Mus musculus
description: The PIQOR Custom_CTID88 Microarray comprises 792 immune related mouse specific cDNAs and 27 rat specific cDNAs. The 792 mouse cDNAs include 6 house-keeping genes as positive controls. Additional positive controls are given by 4 E. coli cDNA fragments, the corresponding in vitro transcribed RNAs (spiking controls) provide a control for the labeling and hybridisation efficiency. 2 negative controls are given by spotted herring sperm DNA and buffer.Spotting in quadruplicate results in 3300 spots.
manufacture_protocol: Defined 200-400 bp fragments of selected cDNAs were generated by RT-PCR, cloned and sequence-verified. Amplified and purified cDNA inserts were spotted in quadruplicate on PIQOR slides using a proprietary piezoelectric spotter. The PIQOR slides were coated in a 2 step process generating a surface which covalently binds DNA molecules.