title: Eide Yeast zinc
status: Public on Jul 04 2008
contact.city: Madison
contact.first: CHANG-YI
contact.last: WU
contact.email: wu4@wisc.edu
contact.state: WI
contact.address: 1415 Linden Dr.
contact.department: Nutritional Sciences
contact.country: USA
contact.institute: UW-Madison
contact.phone: 608-262-1118
organism: Saccharomyces cerevisiae
manufacture_protocol: 1. The PCR amplification products of all the S. cerevisiae open reading frames (ORFs) were purchased from Research Genetics.
  2. Each PCR product was reamplified and purified on glass fiber filter plates (Millipore).
  3. The reamplified products representing 6000 ORFs (4% not represented) were diluted to 50% Me2SO and spotted in duplicate on 3-aminopropyl-methyldiethoxy silane-coated slides using a Generation III Microarray Spotter (Amersham Biosciences).