title: ProtoArray Human Protein Microarray v3.0
status: Public on Feb 13 2008
contact.city: Stanford
contact.first: Li
contact.last: Li
contact.email: li.li@mssm.edu
contact.state: CA
contact.address: 300 pastuer dr.,
contact.department: Pediatric
contact.country: USA
contact.institute: Stanford University
contact.phone: 6507243765
organism: Homo sapiens
manufacture_protocol: The ProtoArray® Human Protein Microarray is a high-density protein microarray containing thousands of purified human proteins for protein interaction screening. Each human open reading frame (ORF) is expressed as an N-terminal GST fusion protein using a baculovirus expression system, purified from insect cells, and printed in duplicate on a nitrocellulose-coated glass slide. The human proteins spotted on the microarray are expressed in insect cells using an optimized process to maximize the production of soluble recombinant proteins
  in a high-throughput format (Schweitzer et al., 2003). Proteins are expressed at high levels in insect cells which are similar to mammalian cells with respect to protein folding and post-translational modifications such as phosphorylation and glycosylation (Bouvier et al., 1998; Hollister et al., 2002; Predki, 2003) in contrast to proteins expressed in E. coli. This allows protein interaction detection at a functional level.
  http://www.invitrogen.com/protoarray