title: UMN Mouse 11K V 11_22_04 BMAP
status: Public on Nov 23 2006
contact.city: Minneapolis
contact.first: Ajay
contact.last: Yekkirala
contact.state: MN
contact.address: 308 Harvard St 8-120
contact.country: USA
contact.institute: University of Minnesota
organism: Mus musculus
manufacture_protocol: Basic protocol for generation of mouse microarrays
  i) Obtain cDNA library
  (1) thousands of bacterial stocks in 96-well plates
  (2) each bacterial stock contains a standard eukaryotic expression
  plasmid that contains a unique stretch of mouse cDNA inserted in
  the multiple cloning site
  ii) Replicate the library
  iii) PCR amplify each cDNA clone in 96 well reactions
  iv) Check PCR reactions on an agarose gel
  v) Purify PCR reactions with a Millipore filter
  vi) Elute purified DNA with 3XSSC (spotting buffer)
  vii) Spot DNA onto glass slides using a DNA spotting robot