title: KCC 23K Mouse Microarray
status: Public on Oct 30 2014
contact.city: Philadelphia
contact.first: My
contact.last: Mahoney
contact.email: my.mahoney@jefferson.edu
contact.state: PA
contact.address: 233 South 10th Street, Suite 428  BLSB
contact.department: Dermatology & Cutaneous Biology
contact.country: USA
contact.institute: Thomas Jefferson University
contact.phone: 215-503-3240
organism: Mus musculus
manufacture_protocol: The oligos were resuspended in 50 mM phosphate buffer pH 8.0 at 20 mM concentration.  The individual oligo-probe was printed on CodeLink activated slides  under 45% humidity by GeneMachine OmniGrid 100 Microarrayer in 4 x 12 pin configuration and 20 x 20 spot configuration of each subarray.  The spot diameter was 100 µm and distance from center to center was 200µm. The microarrays were ready for sample hybridization after additional 100 mM Taurine/Bicine blocking and 4X SSC/0.1% SDS washing steps.