title: Mud crab 6K cDNA array version 1.0
status: Public on Jul 01 2010
contact.city: Xiamen
contact.first: zou
contact.last: zhihua
contact.email: zouzhihua@jmu.edu.cn
contact.state: Fujian
contact.address: NO.43 Yindou road
contact.department: Fishry college
contact.country: China
contact.institute: Jimei University
organism: Scylla paramamosain
manufacture_protocol: 1.Insert DNA of cDNA clones were PCR amplified and purified.
  2.Purified insert DNA was arrayed in 96-well V-bottom plates,dried completely, re-dissolved in spotting solution (50 % DMSO) to a final concentration of 200 ng/μl.
  3. Seal plates with plastics seals.
  4. Incubate at 37°C for 30 minutes to aid resuspension of DNA.
  5. Shake plates near maximum rotational speed on flat-bed shaker for 1 minute.
  6. Centrifuge plates at 1000 rpm for 5 minutes.
  7. Remove seals and cover with plate lids. Place in appropriate location of plate cassette. This should be done with first plates just before print run is started to minimize evaporation time before printing. For second and third cassettes, wait until 24 minutes.
  8.Then the cDNAs are printed onto CapitlBio AminoSlide (CapitalBio, Beijing, China) using a PixSys5500 arrayer (Cartesian Technologies, Irvine, CA, USA) equipped with 16 (4*4) Chipmaker 2 pins (Telechem, Sunnydale, CA)