title: Nimblegen Catfish 28K Oligo Microarray
status: Public on Oct 23 2007
contact.city: Auburn
contact.first: Eric
contact.last: Peatman
contact.email: peatmer@auburn.edu
contact.state: AL
contact.address: 203 Swingle Hall
contact.department: Fisheries and Allied Aquacultures
contact.country: USA
contact.institute: Auburn University
organism: Ictalurus punctatus
  Ictalurus furcatus
description: This platform builds upon a previous 19K channel catfish microarray design (GPL2814). Newly sequenced immune and miscellaneous transcripts from channel catfish were added bringing the number of sequences from that species to 21,359.  Additionally, 7,159 unique ESTs from the closely-related species blue catfish (Ictalurus furcatus) were added.
manufacture_protocol: Nimblegen utilized an in situ maskless array synthesis technology to synthesize 24 base pair (24mer) oligos on the surface of the microarray slides.  At least 6 perfect match and 6 mismatch oligos were designed for each provided sequence using Nimblegen proprietary software.