title: Melon single feature polymorphism array v1.0 187K
status: Public on Aug 01 2010
contact.city: Bet-Dagan
contact.first: Ron
contact.last: Ophir
contact.address: POB 6
contact.department: Fruit Tree Sciences
contact.country: Israel
contact.institute: Agricultire Research Organization
organism: Cucumis melo subsp. melo
manufacture_protocol: Agilent SurePrint technology
  Array was designed using melon ESTs deposited at www.icugi.org. Both catalog and custom microarrays are manufactured using a proprietary non-contact industrial inkjet printing process in which oligo monomers are deposited uniformly onto specially-prepared glass slides. This in situ synthesis process prints up to 60-mer length oligonucleotide probes, base-by-base, from digital sequence files. The precise inkjet process enables the delivery of extremely small, accurate volumes (picoliters) of the chemicals to be spotted. Standard phosphoramidite chemistry used in the reactions allows for very high coupling efficiencies to be maintained at each step in the synthesis of the full-length oligonucleotide.