title: LLHoyer_Calbicans_6k_v1.0
status: Public on Aug 01 2007
contact.city: Urbana
contact.first: Lois
contact.last: Hoyer
contact.state: IL
contact.address: 2001 S Lincoln Ave
contact.department: Pathobiology
contact.country: USA
contact.institute: University of Illinois
organism: Candida albicans
manufacture_protocol: Two separate rounds of PCR were performed on the ORF sequences.  The products were combined and the purity and length of PCR products were viewed by agarose gel electrophoresis.  Samples were dried and resuspended in 3x SSC with an approximate concentration of 0.1 µg/µl of DNA in each PCR well.  A total of 6392 PCR products were spotted (proprietary techniques created by Microarrays Inc., Nashville, TN) in duplicate onto SuperAmine Substrate slides (Telechem Intl).  Controls included Arabidopsis thaliana Spot Report Genes and mammalian DNA fragments (Stratagene), C. albicans genes of known expression profiles, and buffer blanks.