title: JCVI Aspergillus fumigatus AF293 29.9K amplicon array
status: Public on Apr 20 2010
contact.city: Atlanta
contact.first: John
contact.last: Varga
contact.email: j.varga@yahoo.com
contact.state: GA
contact.address: 1510 Clifton Road
contact.department: Pediatrics
contact.country: USA
contact.institute: Emory University
organism: Aspergillus fumigatus Af293
manufacture_protocol: The PCR amplicon microarray was designed based on CDS annotated from the A. fumigatus AF293 whole genome sequence.  The array consists of 29,952 spots.  The spots represent 9796 A. fumigatus AF293 genes spotted in triplicate with the remaining spots being empty.   The PCR products were printed on Corning glass UltraGAPs amino-silane coated slides at JCVI using a high density automated arrayer (Intelligent Automation, Cambridge, MA 02139).  The array consists of spots arranged 26 x 24 grids.  There are 48 grids in 4 columns (meta-columns) and 12 rows (meta-rows).