title: tomato cDNA array (Ye lab)
status: Public on Jan 09 2007
contact.city: Ithaca
contact.first: Zhangjun
contact.last: Fei
contact.state: NY
contact.address: Tower Road
contact.country: USA
contact.institute: Boyce Thompson Institute
organism: Solanum lycopersicum
manufacture_protocol: The amplified cDNA PCR products were precipitated with the addition of 100 μl of anhydrous ethanol and resuspended in 15 μl of 50% dimethyl sulphoxide (DMSO) at a final concentration of 0.1-0.5 μg/μl and then spotted onto amino silaned glass slides (CapitalBio.Corp, Beijing, China) with a SmartArrayerTM microarrayer (CapitalBio Corp.). Each clone was printed in triplicate. After printing the slides were baked for 1 h at 80 °C and stored dry at room temperature