title: CME-Human-3K6_7 (condensed version of GPL6928)
status: Public on Oct 15 2012
contact.city: Brussels
contact.first: Claudia
contact.last: Spits
contact.email: claudia.spits@vub.be
contact.address: Laarbeeklaan 103
contact.department: Research Group of Reproduction and Genetics
contact.country: Belgium
contact.institute: Vrije Universiteit Brussel
organism: Homo sapiens
manufacture_protocol: 1. Microgrid (Genomics Solutions, UK).
  2. 384 microtiter plates (X7022, Genetix)
  3. 16 MicroSpot quill pins - 10K (cat# BR001489, Genomics Solutions, UK)
  4. Codelink slides (cat#: 30001100, GE Healthcare). Printing 2 arrays of 4x4 blocks on 1 microscope slide: 1. Dissolve PCR products in 125mM Phosphate buffer, 0.000125% sarcosyl at concentrations ranging between 300 ng/ul and array in 384 microtiter plates. 2. Set spotter settings for 2 arrays of 4x4 blocks printed with 16 pins: column pitch = 185 um, row pitch = 185 um; Humidity 55%; 61 spots per source visit. 3. Make sure room temperature is between 20-24 degrees Celsius. Place microtiter plates in the plate hotel with A1 in front left corner.
  5. Place 60 to 120 slides on the slide-deck.
  6. Start spotting early in the morning and stop spotting procedure at end of the day and remove pin set. Leave spotter overnight with vacuum and humidity settings on. Continue spotting the next morning until all plates are printed.
  7. After spotting, incubate the slides in humid chamber with saturated NaCl for 24 to 72 hours.
  8. Block 1%NH4OH.
  9. Slides can be stored for at least 6 months in a dry-cabinet.
  Support: glass Coating: aminosilane Description: The array contains BAC and PAC sequences, amplified by DOP - PCR and printed in 2 replicates.