title: UnivMinnesota/Dunny Efaecalis v1.0
status: Public on Dec 01 2013
contact.city: Bethesda
contact.first: Kristi
contact.middle: L
contact.last: Frank
contact.state: MD
contact.address: 4301 Jones Bridge Rd
contact.department: Microbiology and Immunology
contact.country: USA
contact.institute: Uniformed Services University of the Health Sciences
organism: Enterococcus faecalis V583
description: GAPS II coated slides, Corning Incorporated (Corning, NY)
manufacture_protocol: The oligonucleotides used in this array have been described previously: Solheim M, Aakra A, Snipen LG, Brede DA, Nes IF (2009) Comparative genomics of Enterococcus faecalis from healthy Norwegian infants. BMC Genomics 10: 194.
  Oligonucleotides were synthesized by Invitrogen Corporation (Carlsbad, CA).  Oligos were spotted in quadruplicate on GAPS II coated slides (Corning Incorporated, Corning, New York) with a Biorobotics Microgrid II spotter (Digilab, Inc., Holliston, MA) at the University of Minnesota Biomedical Genomics Center Microarray Facility.  Slides were prehybridized at 42°C in prehybridization buffer according to the instructions in the Corning GAPS II manual immediately before use.