title: Compugen Human Oligonucleotide Library 12K
status: Public on Jul 23 2009
contact.city: Nankoku
contact.first: Keisuke
contact.last: Taniuchi
contact.email: jm-ktaniuchi@kochi-u.ac.jp
contact.address: Kohasu, Oko
contact.country: Japan
contact.institute: Kochi University Medical School
contact.phone: +81-88-880-2338
organism: Homo sapiens
description: Arrays were custom-printed with a MagnaSpotter robot (BioAutomation Corp.) by a dedicated core facility located at the Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, NE (by DLK).  They were constructed from a set of sense oligonucleotide (60-mer) probes designed for each human target gene by Compugen Inc., and manufactured by Sigma-Genosys.
manufacture_protocol: Human oligonucleotide probes (60mers) were designed for each target gene (Compugen, manufactured by Sigma-Genosys, Inc). Oligonucleotides (12,135) were resuspended (30µM) in 3X saline sodium citrate (SSC) buffer and spotted onto poly-L-lysine coated slides using a MagnaSpotter robot (BioAutomation) with a 12-pin print head (Telechem) configuration in a humidified, HEPA-filtered hood. After spotting, the DNA was cross-linked to the slides by UV irradiation (350mJ/cm2) with a Stratalinker UV Crosslinker (Stratagene), blocked by succinic anhydride treatment, and rinsed in ethanol. The printed arrays were boxed and stored desiccated at room temperature.