title: Febit GmbH Geniom Biochip MPEA Rattus norvegicus (Sanger miRBase version 14.0 September 2009)
status: Public on Mar 28 2011
contact.city: Berlin
contact.first: Nathanael
contact.last: Raschzok
contact.email: nathanael.raschzok@charite.de
contact.address: Augustenburger Platz 1
contact.department: Department of Surgery; Campus Charite Mitte / Campus Virchow Klinikum
contact.country: Germany
contact.institute: Charité - Universitätsmedizin Berlin
organism: Rattus norvegicus
manufacture_protocol: The febits biochip Geniom Biochip MPEA Rattus norvegicus was used for this analysis. MPEA: On-Chip Labeling for Highest Sensitivity
  Enzymatic labeling assay requires minimal sample amount
  In addition to the standard hybridization of pre-labeled RNA, the microfluidic Geniom Biochip enables a highly sensitive enzymatic labeling protocol.
  MPEA benefits
  * Higher sensitivity and specificity
  * miRNA hybridization without preceding treatment like enrichment, PCR -based amplification or labeling eliminates potential bias
  * High discriminatory power
  * Minimal amount of sample RNA needed (more than or equal 20 ng total RNA)
  * Easy to use protocol for Geniom RT Analyzer
  The unique Microfluidic Primer Extension Assay (MPEA) uses unlabeled miRNAs for hybridization. In a second step, Klenow fragment of DNA polymerase I is added directly into the channels of the microfluidic chip, where the specific elongation of the bound miRNAs takes place.
  The method combines the specificity of a hybridization assay with the discriminatory power of an enzymatic extension and reduces the required sample amount to a minimum.
  Details provided at www.febit.com