title: Drosophila_Melanogaster_NCSU_ARRAY
status: Public on Dec 17 2008
contact.city: Raleigh
contact.first: Stan
contact.last: Martin
contact.state: NC
contact.address: 1404 Crete Dr.
contact.country: USA
contact.institute: Science Apps, L3C
organism: Drosophila melanogaster
description: We constructed the cDNA microarrays using 4,256 expressed sequence tags (ESTs) from a library sequenced by the Berkeley Drosophila Genome Project (BDGP) and provided by Research Genetics, which were reamplified by  PCR in 96-well format in the Genome Research Laboratory at North Carolina State University. We transferred clones that showed a strong, single band to 384-well plate format, and spotted the DNA onto ArrayIt SuperAmine slides (Telechem) at a spacing of 225 m using an Affymetrix 417 Arrayer with the Pin and Ring method25 (Genetic Microsystems) that results in relatively uniform DNA deposition within 125 m diameter spots. We then cross-linked the DNA to the slides with 1,200 J of irradiation in a UV Stratalinker (Stratagene) and processed the slides according to the manufacturer's recommendations
manufacture_protocol: Clones were spotted onto ArrayIt SuperAmine slides (Telechem) at a spacing of 225 nm using an Affymetrix 417 Arrayer with the Pin and Ring method25 (Genetic Microsystems) that results in relatively uniform DNA deposition within 125 m diameter spots.