title: University of Alberta Escherichia coli 3x6K microarray
status: Public on Mar 02 2011
contact.city: Edmonton
contact.first: Michael
contact.last: Gänzle
contact.address: 4-10 Ag/For Centre
contact.department: AFNS
contact.country: Canada
contact.institute: University of Alberta
organism: Escherichia coli
manufacture_protocol: The E. coli slide produced contains 5,978 oligos and a control set of 12 positive and 12 negative controls in a 4 by 4 block of sub-arrays which are printed in triplicate filling the slide surface with a total of 19086 spots printed at 200 um spacing in both the horizontal and vertical direction.
  
  The Oligos have been dissolved to a final concentration of 18.75 uM in 150 mM NaPO4 buffer (pH 8.7) prior to printing. The slides used are Corning Epoxide slides which have been chosen for ease of use, low background, and consistantly exceptional results.
  
  The Operon E. coli Genome Oligo Set version 1.0 consists of 5,978 70mer probes representing three strains of E. coli. The number of open reading frames represented is as follows: 4,289 ORF's in K12, 5,029 ORF's in O157:H7 (EDL933), and 4,836 ORF's in O157:H7 (Sakai). All 5,978 probes are designed within ORFs from both clones and predicted ORF's. For this probe set design, Operon employed its state-of-the-art methods and proprietary software.
  
  All probes are Tm normalized to 75oC (+/- 5oC) and have a 5' amino linker.