title: OSUrbt Oncorhynchus mykiss 1.6K v2.0
status: Public on Sep 06 2005
contact.city: Corvallis
contact.first: Susan
contact.middle: C.
contact.last: Tilton
contact.email: Susan.Tilton@oregonstate.edu
contact.state: OR
contact.address: 1007 ALS Bldg
contact.department: AG Envr & Molec Toxicology
contact.country: USA
contact.institute: Oregon State University
organism: Oncorhynchus mykiss
description: 4 X 4 pin arrangement Microspot 2500 pins (quill design)
manufacture_protocol: Oligonucleotides were resuspended in spotting buffer (3X SSC and 1.5 M betaine) at 25 uM final concentration and were printed onto Corning UltraGap slides (Acton, MA) using a Biorobotics Microgrid II arrayer (Genomic Solutions, Ann Arbor, MI) with 16 pins.  The trout 70-mers were each printed in duplicate and a set of ten SpotReport Alien Oligos (Stratagene, La Jolla, CA) were printed 16 times (once per block) on the array.  After printing, slides were desiccated for 24-48 hrs and cross-linked with UV Stratalinker (Stratagene) at 600 mJ.