title: Dunaliella sp. 0.8 Kb cDNA microarray platform
status: Public on Jan 01 2010
contact.city: Seoul
contact.first: MinJung
contact.last: Kim
contact.email: kiola@hanyang.ac.kr
contact.address: 17 Haengdang-dong, Seongdong-gu
contact.department: Life Science
contact.country: South Korea
contact.institute: Hanyang University
contact.phone: 82-2-2220-2561
organism: Dunaliella salina
manufacture_protocol: We constructed the cDNA microarrays from the Dunaliella sp. cDNA library (Park et al., 2006). 1052 selected expressed sequence tags (ESTs) from each clone, including housekeeping genes like ribosomal binding proteins, were amplified by PCR using M13 primers and recovered by a MultiScreen PCR Filter Plate (Millipore, Billerica, MA, USA). Each cDNA chip was designed with 778 successfully amplified PCR products from the cDNA library. Additionally, several control clones from the Saccharomyces cerevisiae intergeneic sequence and the Dunaliella sp. housekeeping genes were included. Microarrays were produced using a fully automated system by robotic operation at Digital Genomics (Seoul, South Korea). The cDNA PCR products were spotted onto amine-coated GAPSII slides (Corning Inc., Corning, NY, USA) using MG II (Biorobotics Inc., Woburn, MA, USA) with a 240 um pitch between spots as a twin format. There were 14 x 15 spots per block, and the spot diameter was 100~140 um. To evaluate fabrication, a cDNA microarray was stained with Syto61 (Molecular Probes, Eugene, OR, USA).