NIH_SPENDING_CATS: Bioengineering;Biomedical Imaging

ORG_CITY: ATLANTA

ORG_NAME: GEORGIA INSTITUTE OF TECHNOLOGY

PHR: PROJECT NARRATIVE The proposed research program is relevant to public health because it advances imaging science and technology to transform biomedical research. The results will open up novel methodological pathways and illuminate both fundamental and translational discoveries. The proposed research is relevant to the part of NIGMS’s mission that it advances basic biomedical research by supporting the development of biomedical technologies to enable studies that underlie health and disease.

PI_NAMEs: JIA, SHU  (contact)

PROGRAM_OFFICER_NAME: YEH, ALVIN TIEN-WEI

PROJECT_TERMS: 3-Dimensional;Address;Biological;Biological Sciences;biological systems;Biology;Biomedical Research;Biomedical Technology;Biophotonics;Cells;Development;Disease;Engineering;Foundations;Goals;Health;Heterogeneity;high dimensionality;Image;Image Enhancement;Imaging Device;imaging platform;imaging science;Imaging technology;in vivo imaging;Infrastructure;innovation;Instruction;Investigation;Laboratories;Leadership;Light;Medical;Methodology;Methods;Microscopy;Mission;Modality;nanoscale;National Institute of General Medical Sciences;novel;Organelles;parent project;Pathway interactions;Physics;Population;programs;Public Health;Research;Research Support;Resolution;restraint;Role;Science;superresolution microscopy;System;Technology;Therapeutic;Time;Tissues;Translations;ultra high resolution;Variant

PROJECT_TITLE: Toward Systems Biophotonics: Imaging Biology across High Dimensions and Scales


ADMINISTERING_IC: DA

ARRA_FUNDED: N

ED_INST_TYPE: SCHOOLS OF MEDICINE

FUNDING_MECHANISM: NON-SBIR/STTR RPGS

IC_NAME: NATIONAL INSTITUTE ON DRUG ABUSE

NIH_SPENDING_CATS: Basic Behavioral and Social Science;Behavioral and Social Science;Brain Disorders;Drug Abuse (NIDA only);Neurosciences;Substance Misuse

ORG_CITY: WORCESTER

ORG_COUNTRY: UNITED STATES

ORG_DEPT: NEUROSCIENCES

ORG_FIPS: US

ORG_NAME: UNIV OF MASSACHUSETTS MED SCH WORCESTER

ORG_STATE: MA

PHR: Narrative: Addiction poses an enormous health and financial burden on our society. Currently, our understanding of the brain circuitries involved in addiction is far from complete. The successful completion of these proposed studies would result in important new information about neurons that may be involved in addiction, potentially creating new targets for therapeutic development.

PI_NAMEs: WINDER, DANNY G. (contact)

PROGRAM_OFFICER_NAME: BERTON, OLIVIER

PROJECT_TERMS: addiction;Adrenergic Agonists;Adrenergic Receptor;Affective;Alleles;Anatomy;Animal Model;Behavior;Behavior Control;Behavioral;behavioral outcome;behavioral response;Brain;brain circuitry;Brain region;Cells;Clinical effectiveness;Cocaine;conditioned place preference;Coupled;Coupling;Cyclic AMP;Cyclic AMP-Dependent Protein Kinases;Cyclic Nucleotides;cyclic-nucleotide gated ion channels;Data;designer receptors exclusively activated by designer drugs;Development;drug action;drug of abuse;drug seeking behavior;experimental study;Fiber;Financial Hardship;Fire - disasters;Fluorescence;fluorescence lifetime imaging;Fluorescent in Situ Hybridization;Funding;genetic approach;Genetic Recombination;Goals;Guanfacine;Health;Heterotrimeric GTP-Binding Proteins;Human;Immunohistochemistry;in vivo;insight;Light;Maps;Measures;Microscopy;Monitor;Mus;Nature;neurochemistry;neuronal circuitry;Neurons;Neurosciences;new therapeutic target;noradrenergic;optogenetics;Pharmaceutical Preparations;pharmacologic;Phenocopy;Photometry;Population;Population Characteristics;Population Heterogeneity;preference;Process;receptor;recruit;Regulation;Relapse;relapse prevention;Reporter;Role;Shapes;Signal Transduction;small hairpin RNA;Societies;Stimulus;Stress;stress reduction;stressor;Structure of terminal stria nuclei of preoptic region;System;Testing;Therapeutic;therapeutic development;Therapeutic Studies;therapy outcome;Time;Viral;Work

PROJECT_TITLE: Noradrenergic Regulation in the BNST

STUDY_SECTION: MNPS

STUDY_SECTION_NAME: Molecular Neuropharmacology and Signaling Study Section[MNPS]


ADMINISTERING_IC: GM

ED_INST_TYPE: BIOMED ENGR/COL ENGR/ENGR STA

IC_NAME: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

NIH_SPENDING_CATS: Genetics;Nutrition;Prevention

ORG_CITY: SALT LAKE CITY

ORG_DEPT: BIOSTATISTICS & OTHER MATH SCI

ORG_NAME: UNIVERSITY OF UTAH

ORG_STATE: UT

PHR: Project Narrative Early-life environmental factors can have long-term health consequences. For example, poor diet and nutrition in utero are associated with increased rates of cardiovascular and metabolic diseases in adults. This Administrative Supplement will enable experiments in a genetic model organism to investigate how diet influences development.

PI_NAMEs: WERNER, MICHAEL S (contact)

PROGRAM_OFFICER_NAME: CHOW, CHI-WING

PROJECT_TERMS: Address;Administrative Supplement;Adolescent;Adopted;Adult;Affect;Animals;Arylsulfatases;Award;Cardiovascular Diseases;cell type;Cells;Chromatin;contrast imaging;cost;CRISPR/Cas technology;Crowding;Deacetylation;Denticle;Detection;Development;developmental plasticity;Diet;Diet and Nutrition;dietary;differential expression;Disease;Environment;Environmental Risk Factor;Epigenetic Process;Evolution;experimental study;feeding;fetal;fitness;Fluorescence;Food;Funding;Gene Expression;Gene Fusion;Genes;Genetic;Genetic Models;Genetic Screening;Genetic study;Genetic Transcription;Genotype;good diet;Health;Histone Acetylation;Histone Deacetylase;Histone Deacetylase Inhibitor;histone modification;Human;Human Development;in utero;instrument;interest;Knock-out;Knowledge;Laboratories;Laboratory Study;Life;Link;Metabolic;Metabolic Diseases;Metabolism;Microinjections;Microscope;Microscopy;model organism;Modeling;Modification;Molecular;Morphology;Mutagenesis;mutant;Nature;Nematoda;Non-Insulin-Dependent Diabetes Mellitus;novel;nutrition;Nutritional;Oral cavity;Organism;Outcome;Pattern;Phenotype;Postdoctoral Fellow;Prevention strategy;Price;promoter;Regulation;Reporter;Resolution;Risk;Series;Shapes;Specificity;Speed;Switch Genes;System;Tail;targeted treatment;theories;Tissues;tool;Tooth structure;trait;Trichostatin A;Unhealthy Diet;Work

PROJECT_TITLE: Chromatin and metabolic regulation of plasticity in a predatory nematode

STUDY_SECTION_NAME: Special Emphasis Panel[ZRG1(55)-R]


FUNDING_MECHANISM: RESEARCH CENTERS

NIH_SPENDING_CATS: Building Research Capacity at Institutions with Limited NIH Funding

ORG_CITY: OKLAHOMA CITY

ORG_NAME: UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR

ORG_STATE: OK

PI_NAMEs: AKINS, DARRIN R. (contact)

PROGRAM_OFFICER_NAME: ARORA, KRISHAN


ED_INST_TYPE: SCHOOLS OF ARTS AND SCIENCES

NIH_SPENDING_CATS: Cannabinoid Research;Cardiovascular;Chronic Pain;Complementary and Integrative Health;Heart Disease;Natural Products;Pain Research;Therapeutic Cannabinoid Research

ORG_CITY: NEW YORK

ORG_DEPT: BIOLOGY

ORG_NAME: HERBERT H. LEHMAN COLLEGE

ORG_STATE: NY

PHR: There is a pressing need to find new compounds to help manage pain due to the ongoing opioid epidemic, and plants, being a source of other analgesic drugs, provide a useful starting point for discovering such compounds. Aconitum is a plant species used in traditional Chinese medicine that produces analgesic diterpenoid alkaloids, while American medicinal Aconitum species also produce diterpenoid alkaloids, but have not been explored in detail for analgesic properties. This project will analyze eight Aconitum species, specifically regarding their analgesic diterpenoid alkaloids that lack cardiotoxicity, with the goal of identifying lead compounds for future preclinical studies.

PI_NAMEs: KENNELLY, EDWARD JAMES (contact)

PROGRAM_OFFICER_NAME: CUPIT, PAULINE

PROJECT_TERMS: Aconitum;Alkaloids;American;Analgesics;Animals;Anti-Inflammatory Agents;Asian;Biological;Biological Assay;Cannabidiol;cannabinoid receptor;Capsaicin;Cardiotoxicity;career development;Chemical Structure;Chemistry;Chinese Traditional Medicine;Data;Diterpenes;endogenous cannabinoid system;Family;Future;Goals;graduate student;Lead;Manuscripts;Medicine;metabolomics;Modernization;Molecular;Natural Products;Natural Products Chemistry;North America;novel;novel therapeutics;opioid epidemic;Opioid Receptor;Pain;Pain management;Pattern;Pharmaceutical Preparations;Plant Extracts;Plants;preclinical study;Property;Ranunculaceae;Research;response;Salicylic Acids;Source;symposium;System;Technology;Testing;Therapeutic;Traditional Medicine;Training;undergraduate student;Underrepresented Populations;Variant;Zebrafish

PROJECT_TITLE: Discovery of analgesic diterpenoid alkaloids from medicinal Aconitum plants using a metabolomic approach


NIH_SPENDING_CATS: Bioengineering;Cardiovascular;Heart Disease;Machine Learning and Artificial Intelligence;Networking and Information Technology R&D (NITRD)

ORG_CITY: Newark

ORG_DEPT: PHYSICS

ORG_NAME: UNIVERSITY OF DELAWARE

ORG_STATE: DE

PHR: Project narrative Selective and rapid transport of K+ ions across the cell membrane is controlled by potassium channels - trans- membrane proteins whose disfunction is linked to cardiac arrhythmias, epilepsies, and other channelopathies. Multidimensional ultrafast vibrational spectroscopy is a powerful technique for studying structure and dynamics of proteins. The GPU cluster we plan to acquire will significantly accelerate our proposed research designed to provide novel machine-learning-based framework for simulating vibrational spectra of proteins which will help interpret and design vibrational spectroscopy experiments aiming at elucidating the molecular underpinnings of activation and conduction of ion channels.

PI_NAMEs: KANANENKA, ALEXEI  (contact)

PROGRAM_OFFICER_NAME: NIE, ZHONGZHEN

PROJECT_TERMS: Acceleration;Administrative Supplement;Architecture;Arrhythmia;Award;Cell membrane;Collaborations;conformational conversion;design;Dimensions;Epilepsy;equipment acquisition;experimental study;Functional disorder;Generations;Goals;Integral Membrane Protein;Ion Channel;Ions;Link;Machine Learning;machine learning framework;machine learning model;Modeling;Molecular;Molecular Conformation;molecular dynamics;Molecular Machines;novel;Physiological;Platinum;Potassium Channel;Protein Dynamics;Proteins;Research;Research Design;response;simulation;Spectrum Analysis;Structure;System;Techniques;Technology;two-dimensional;voltage;Water

PROJECT_TITLE: PowerEdge XE8640 GPU cluster for molecular dynamics simulations and machine learning computational vibrational spectroscopy of ion channels


NIH_SPENDING_CATS: Behavioral and Social Science;Brain Disorders;Clinical Research;Clinical Trials and Supportive Activities;Drug Abuse (NIDA only);Health Disparities Research;Health Disparities and Racial or Ethnic Minority Health Research;Opioid Misuse and Addiction;Opioids;Social Determinants of Health;Substance Misuse

ORG_CITY: COLLEGE PARK

ORG_NAME: UNIV OF MARYLAND, COLLEGE PARK

ORG_STATE: MD

PHR: Project Narrative Opioid use disorder (OUD) continues to be a significant problem in urban areas disproportionately affecting lower income and minoritized populations. The findings from this study will provide an understanding of the neighborhood characteristics of urban communities and their influence on treatment outcomes for OUD. The findings from this study can contribute to future research and interventions that will focus on the larger and systematic issues prevalent in urban communities that impact OUD treatment retention.

PI_NAMEs: MAGIDSON, JESSICA F (contact)

PROGRAM_OFFICER_NAME: MARTIN, LINDSEY ANN

PROJECT_TERMS: Address;Affect;Alcohol consumption;Applications Grants;Area;Assessment tool;Award;Baltimore;Behavior Therapy;behavioral clinical trial;Black race;career;Characteristics;Cities;Clinic;Clinical Trials Design;Communities;Data;design;Disease;Drops;Drug usage;Educational workshop;Effectiveness;Ethnic Origin;Future;Generations;Goals;Grant;Health;Health Services Accessibility;Humanities;improved;innovation;Intervention;Interview;Life;Low income;medication for opioid use disorder;Mentors;Mentorship;Methadone;methadone treatment;Minority Groups;Modernization;Neighborhoods;Opioid;opioid treatment program;opioid use disorder;Outcome;Overdose;parent project;Participant;peer;Pharmaceutical Preparations;Postdoctoral Fellow;Public Health;Recovery;recruit;Relapse;Research;Research Methodology;skills;social deficits;social health determinants;Social Sciences;statistical learning;Structure;Training;Treatment outcome;United States National Institutes of Health;urban area;Urban Community;Violence;Woman;Work

PROJECT_TITLE: The influence of neighborhood factors and social determinants of health on OUD treatment outcomes

STUDY_SECTION: NSS

STUDY_SECTION_NAME: NSS


NIH_SPENDING_CATS: Biotechnology;Genetics;Human Genome

ORG_CITY: CHAPEL HILL

ORG_DEPT: BIOCHEMISTRY

ORG_NAME: UNIV OF NORTH CAROLINA CHAPEL HILL

ORG_STATE: NC

PHR: PROJECT NARRATIVE The goal of this project is to elucidate fundamental knowledge of the link between three-dimensional genome organization and gene expression. This research will investigate the properties of cohesin complexes, identify and characterize novel cohesin-interacting proteins, and elucidate the role of cohesin-mediated DNA loops in regulation of transcription and chromatin state. This knowledge is critical for understanding human health and disease states linked to altered three-dimensional genome organization, such as developmental disorders and cancers.

PI_NAMEs: DOWEN, JILL  (contact)

PROGRAM_OFFICER_NAME: VATAKIS, DIMITRIOS NIKOLAOS

PROJECT_TERMS: 3-Dimensional;Acute;Binding;Biochemical;Biological Assay;Biology;CCCTC-binding factor;Cell Differentiation process;Cells;Chromatin;Chromatin Loop;cohesin;Complex;Defect;developmental disease;Disease;embryonic stem cell;Enhancers;Epigenetic Process;experience;functional genomics;Gene Expression;Gene Expression Regulation;Genetic;Genetic Transcription;Genome;genome editing;Genomics;Goals;Health;histone modification;Human;interdisciplinary approach;Knowledge;Link;Malignant Neoplasms;Mediating;Molecular;novel;promoter;Property;Proteins;Proteomics;Research;Role;Site;small molecule;Technology;Testing;Transcriptional Regulation;Variant

PROJECT_TITLE: The role of genome folding in regulating gene expression and chromatin state


NIH_SPENDING_CATS: Genetics;Human Genome

ORG_DEPT: GENETICS

PHR: Project Narrative Replication of the DNA within our genome requires a highly orchestrated series of molecular events to propagate genetic information during cell proliferation with high fidelity, and thus to suppress the onset of diseases such as cancer. This project will combine a unique genetic strategy in the fruit fly Drosophila melanogaster with sophisticated imaging and molecular biology approaches to determine how the synthesis of histones proteins and their packaging of DNA into chromatin regulates the process and fidelity of DNA replication. Because of the high evolutionary conservation of regulators of chromatin and DNA replication, discoveries resulting from our mechanistic studies in flies will be directly applicable to humans.

PI_NAMEs: DURONIO, ROBERT J (contact)

PROGRAM_OFFICER_NAME: REDDY, MICHAEL K

PROJECT_TERMS: Acetylation;Address;Anabolism;Animals;Base Pairing;Biochemical;Biological;Cell Cycle;Cell Cycle Regulation;Cell Proliferation;Cells;Chemicals;Chromatin;Chromatin Modeling;Chromosomes;Development;DNA;DNA biosynthesis;DNA Packaging;DNA sequencing;Drosophila genus;Drosophila melanogaster;Engineering;Epigenetic Process;Event;fly;Gene Cluster;Gene Expression;Genes;genetic approach;genetic information;Genetic Transcription;Genome;Health;Histones;Homeostasis;Human;Human Pathology;Image;in vivo;Individual;Location;Malignant Neoplasms;Messenger RNA;Methods;Methylation;Modification;Molecular;Molecular Biology;mRNA Precursor;Mutation;N-terminal;next generation;Nuclear;Nucleosome Core Particle;Nucleosomes;Onset of illness;Phosphorylation;Play;Poly(A) Tail;prevent;Process;Proteins;Regulation;Role;S phase;Series;stem;System;Tail;whole genome

PROJECT_TITLE: Epigenetic Control of the Cell Cycle During Animal Development

STUDY_SECTION: MRAC

STUDY_SECTION_NAME: Maximizing Investigators' Research Award C Study Section[MRAC]


NIH_SPENDING_CATS: Aging;Bioengineering;Biomedical Imaging;Nanotechnology;Stem Cell Research;Stem Cell Research - Embryonic - Human

ORG_CITY: PHILADELPHIA

ORG_NAME: RESEARCH INST OF FOX CHASE CAN CTR

ORG_STATE: PA

PHR: Project Narrative Phase separation is a common cell biology phenomenon with unsolved implications in human aging and neurodegenerative diseases. Specialized instruments, such as the NanoLive microscope, can visualize phase- separated cell structures in living cells for a long period at unprecedented resolution, without harming cells. The use of it in our program will lead to new knowledge in phase separation biology and likely new drug targets to treat aging-related symptoms.

PI_NAMEs: CHEN, LU  (contact)

PROJECT_TERMS: 3-Dimensional;Aging;aging related;Biogenesis;Biological Assay;Biology;Cell Cycle Progression;Cell Death;cell fixing;cell killing;Cell Line;Cells;Cellular biology;Cellular Structures;chimeric antigen receptor T cells;Contract Services;Funding;Grant;high throughput screening;Human;human embryonic stem cell;human imaging;Imprisonment;instrument;interest;Investigation;Knowledge;Label;Life;manufacture;Microscope;Modeling;Morphology;movie;nanoscope;National Institute of General Medical Sciences;Nature;Neurodegenerative Disorders;new therapeutic target;Nuclear;Nuclear Structure;Optical Tomography;Organelles;Phase;Phototoxicity;Process;programs;reconstitution;Research;Resolution;RNA;Site;stem cell fate specification;Switzerland;Symptoms;System;Techniques;Telomerase;telomere;United States National Institutes of Health;Visualization

PROJECT_TITLE: Telomerase RNP Prisonbreaks from Phase-Separated Nuclear Body


NIH_SPENDING_CATS: Neurosciences

ORG_CITY: SAINT LOUIS

ORG_DEPT: ANESTHESIOLOGY

ORG_NAME: WASHINGTON UNIVERSITY

ORG_STATE: MO

PHR: Program Narrative Neurosteroids and cholesterol are important signaling molecules that modulate the function of integral membrane proteins. The proposed research will define the specific binding sites for neurosteroids on various forms of their major target protein, the GABA type A receptor and for cholesterol on lipid scramblases and lysosomal signaling proteins. The work will identify site-specific agonists and antagonists for the sterol binding sites and provide new insights into the biological role of neurosteroids and cholesterol as allosteric modulators of protein function.

PI_NAMEs: EVERS, ALEX S. (contact)

PROGRAM_OFFICER_NAME: JUSTINOVA, ZUZANA

PROJECT_TERMS: Affinity;Agonist;Amino Acid Substitution;Aminobutyric Acids;Anesthetics;antagonist;anti-depressive agents;Behavioral;Binding;Binding Sites;Biological;Biological Assay;Cholesterol;cholesterol analog;Cryoelectron Microscopy;Dependence;Event;Fluorescence;FRAP1 gene;gamma-Aminobutyric Acid;Goals;insight;Integral Membrane Protein;Laboratory Study;Ligands;Lipids;Mass Spectrum Analysis;Measures;Mediating;Membrane Proteins;Methods;Molecular Conformation;monomer;nervous system development;neuronal excitability;Neuroprotective Agents;neurosteroids;novel;programs;Protein Chemistry;protein expression;protein function;protein phosphatase inhibitor-2;Proteins;Reader;receptor;Research;Resolution;Role;screening;sedative;Signaling Molecule;Signaling Protein;Site;small molecule;Specificity;Sterols;Structure;Synapses;Techniques;Therapeutic;tool;Work

PROJECT_TITLE: Neurosteroid and Cholesterol Binding to Integral Membrane Proteins

STUDY_SECTION: MRAB

STUDY_SECTION_NAME: Maximizing Investigators’ Research Award B Study Section[MRAB]


NIH_SPENDING_CATS: Cancer;Congenital Structural Anomalies;Osteoporosis;Pediatric;Rare Diseases

ORG_CITY: Columbus

ORG_DEPT: CHEMISTRY

ORG_NAME: OHIO STATE UNIVERSITY

ORG_STATE: OH

PHR: PROJECT NARRATIVE Plastin family of the actin cytoskeleton organizers contributes to the activation of immune cells, bone formation, cell division, migration, and adhesion as well as to shaping biological membranes essential for the absorbance of nutrients by the intestine, blood dialysis by kidneys, and audial perception by the inner ear. When disrupted or expressed ectopically, plastins contribute to the metastatic activity of cancer cells, autoimmune processes, congenital osteoporosis, syndrome of diaphragmatic hernia, and deafness. Our investigation will reveal fundamental molecular mechanisms underlying the activity of human plastins and will generate a strong and lasting impact on the fundamental understanding of the actin cytoskeleton leading to potential therapeutic interventions for cancers, familial osteoporosis, and autoimmune disorders.

PI_NAMEs: KUDRYASHOV, DMITRI  (contact)

PROGRAM_OFFICER_NAME: AINSZTEIN, ALEXANDRA M

PROJECT_TERMS: 3-Dimensional;Actin-Binding Protein;Actins;Adhesions;Age;Allosteric Regulation;Architecture;Attenuated;Autoimmune Diseases;Autoimmune Process;Binding;Biochemical;Biological;Blood;bone fragility;Bundling;Calcium;calponin;cancer cell;Cancer Intervention;cancer invasiveness;Cell Adhesion;Cell division;cell motility;Cell Physiology;Cells;cellular microvillus;Chronic Lymphocytic Leukemia;Communication;Communities;Complex;congenital deafness;congenital hearing loss;Connective Tissue Diseases;coronin protein;Coupling;crosslink;Crosslinker;Cryo-electron tomography;Cryoelectron Microscopy;Cytokinesis;Cytoskeletal Modeling;Cytoskeleton;Data;deafness;Development;Diaphragmatic Hernia;Disease;Disseminated Malignant Neoplasm;Endocytosis;Epithelium;Event;F-Actin;Family;Fiber;Filopodia;Fimbrin;flexibility;Fluorescence;Focal Adhesions;Fostering;functional outcomes;Goals;Health;hearing impairment;Human;Human Activities;immune activation;Immune system;immunological synapse formation;improved;Individual;interest;Intestines;Investigation;Kidney;Labyrinth;LCP1 gene;Lead;Libraries;Link;Malignant Neoplasms;Maps;Mediating;member;Membrane;Microfilaments;Microscopy;migration;Modeling;Molecular;Molecular Conformation;molecular dynamics;mutant;Mutation;Nutrient;Osteogenesis;Osteoporosis;Pathology;Perception;Personal Satisfaction;plastin;Post-Translational Protein Processing;Process;Property;protein crosslink;Protein Isoforms;Proteins;Publishing;reconstruction;recruit;Recycling;Regulation;Renal dialysis;Research;Resolution;response;Role;Shapes;Signal Transduction;single molecule;Site;stem;Structural Models;Structure;Syndrome;Testing;Therapeutic;Therapeutic Intervention;Tissues;Translating;Tropomyosin;Variant;Work;X-Ray Crystallography

PROJECT_TITLE: Molecular and cellular mechanisms of the actin cytoskeleton organization and function


NIH_SPENDING_CATS: Bioengineering;Biotechnology;Data Science;Machine Learning and Artificial Intelligence;Networking and Information Technology R&D (NITRD)

ORG_CITY: BLACKSBURG

ORG_NAME: VIRGINIA POLYTECHNIC INST AND ST UNIV

ORG_STATE: VA

PHR: PROJECT NARRATIVE: Genomic-scale annotation of protein structures is of vital importance in contemporary biomedical research for understanding the molecular basis of complex diseases and facilitating structure-based drug design. This project will acquire a Dell high-performance computing server with 8 Nvidia H100 Graphics Processing Units (GPUs) to supercharge foundational deep learning method development for scalable and accurate prediction of protein structures, thus accelerating drug discovery and improving therapeutic strategies.

PI_NAMEs: BHATTACHARYA, DEBSWAPNA  (contact)

PROGRAM_OFFICER_NAME: GERSHENSON, ANNE

PROJECT_TERMS: Acceleration;Address;Artificial Intelligence;Biomedical Research;Complex;Computer Models;Data;deep learning;deep learning model;Development;Disease;Drug Design;drug discovery;Genomics;Grant;High Performance Computing;Homologous Protein;improved;Instruction;Memory;method development;Methods;Modeling;Molecular;molecular scale;neural network architecture;Orphan;Parents;Positioning Attribute;Productivity;Protein Databases;protein structure;protein structure prediction;Proteins;Publications;Research;Resolution;Resources;Sequence Homologs;Speed;Structure;Tertiary Protein Structure;Testing;Therapeutic;Time;tool;Training;Work

PROJECT_TITLE: GPU-accelerated high-performance computing to supercharge foundational deep learning method development for scalable and accurate prediction of protein structures


NIH_SPENDING_CATS: Bioengineering;Building Research Capacity at Institutions with Limited NIH Funding;Data Science;Machine Learning and Artificial Intelligence;Networking and Information Technology R&D (NITRD)

ORG_CITY: HATTIESBURG

ORG_NAME: UNIVERSITY OF SOUTHERN MISSISSIPPI

ORG_STATE: MS

PHR: Computation of biological data benefits from access to contemporary environments and equipment. Cloud computing offers a solution to relentless need to modernize and expand computational resources. This project will provide cloud computing lessons for the undergraduate classroom so students can begin their careers using the most future proof option.

PI_NAMEs: FLYNT, ALEX  (contact)

PROGRAM_OFFICER_NAME: ZHOU, YANG

PROJECT_TERMS: Adopted;Applications Grants;Area;Artificial Intelligence;Bioinformatics;Biological;Biometry;Black Box;career;Chemistry;cloud based;Cloud Computing;cloud platform;Code;college;computing resources;cost;Data;Data Collection;data collection site;data curation;data handling;Data Science;Data Science Core;Data Set;data structure;deep learning;design;Discipline of Nursing;Education;Educational Curriculum;electron density;electronic structure;Electronics;Environment;Epidemiology;epidemiology study;Equipment;experience;explainable machine learning;Exposure to;Faculty;faculty support;Familiarity;Fees;Foundational Skills;Funding;Funding Opportunities;Future;Goals;health data;Health Occupations;High Performance Computing;Infrastructure;instructor;interest;Learning;Learning Module;light weight;Machine Learning;machine learning method;Maps;Mathematics;Methods;Minor;Mississippi;Modeling;Modernization;Molecular;molecular modeling;National Institute of General Medical Sciences;Natural Language Processing;Occupations;Participant;Physics;Potential Energy;programs;Proteins;Public Health;Publishing;repository;Research;Research Personnel;Resources;response;Science;Scientist;skills;small molecule;statistics;structural biology;Structure;Students;Surface;System;Time;tool;Training;Training Activity;Trees;undergraduate student;Universities;Work

PROJECT_TITLE: Mississippi INBRE


ADMINISTERING_IC: NS

IC_NAME: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE

NIH_SPENDING_CATS: Genetics;Neurosciences

ORG_CITY: ALBANY

ORG_NAME: STATE UNIVERSITY OF NEW YORK AT ALBANY

PHR: Project Narrative There is a growing interest in the different types of non-canonical translation, including repeat associated non- AUG translation which is associated with RNA structure, translation in multiple frames and microsatellite expansion diseases. Having identified other forms of multi-frame non-canonical translation outside of RAN translation, the overarching goal of this proposal is to determine the RNA structures, protein networks and therapeutically relevant modifiers that govern different types of multi-frame translation. Together this information will provide a deeper understanding of the mechanism of multi-frame translation and the processes that govern human cellular proteome diversity, which in turn will enable informed therapy development across multiple microsatellite expansion diseases.

PI_NAMEs: SHORROCK, HANNAH  (contact)

PROGRAM_OFFICER_NAME: RANGANATHAN, SRIKANTH

PROJECT_TERMS: Biology;CAG repeat;Cell Line;Clustered Regularly Interspaced Short Palindromic Repeats;comparative;Coupled;Data;design;Detection;Disease;disease model;Genetic;genome wide screen;genome-wide;Goals;Human;Human Genome;Immunologics;Induced pluripotent stem cell derived neurons;Initiator Codon;innovation;interest;Knowledge;Mass Spectrum Analysis;Mediating;Methods;Microsatellite Repeats;Modeling;Molecular;molecular modeling;Mutagenesis;Nervous System Disorder;novel;Pathogenesis;Pathogenicity;Peptide Initiation Factors;permissiveness;Play;polyglutamine;polysome profiling;Process;Production;Proteins;Proteome;ran GTP-Binding Protein;Reading Frames;Reporter;Research;Ribosomes;RNA;Role;Sedimentation process;Small Interfering RNA;Structure;System;Testing;Therapeutic;Therapeutic Intervention;therapy development;Tissues;Transcript;translation factor;Translations;Treatment Efficacy;Untranslated RNA;Western Blotting

PROJECT_TITLE: Mechanistic Basis for Non-Canonical Translation in Neurological Disease

STUDY_SECTION: NST

STUDY_SECTION_NAME: NST-2 Study Section [NST-2]


ED_INST_TYPE: OVERALL MEDICAL

NIH_SPENDING_CATS: Neurodegenerative;Neurosciences

ORG_CITY: SAN JUAN

ORG_NAME: UNIVERSITY OF PUERTO RICO MED SCIENCES

ORG_STATE: PR

PHR: UPR COBRE Center for Neuroplasticity During Phase 3 the UPR Center for Neuroplasticity will continue to provide a multidisciplinary cadre of investigators with cutting-edge facilities, scientific capability, and multidisciplinary collaborations for the treatment of neurodegeneration-related diseases. Our projects are unified by shared thematic and technical factors toward understanding and treating neurological disorders, including, the origins of neural and mental health disorders, as well as elucidating the molecular basis for alcohol neuroadaptation, addiction, and neuroprotection. Our center will become a world-recognized center for the study of neurodegeneration-related diseases with therapeutic applications.

PI_NAMEs: LASALDE-DOMINICCI, JOSE ANTONIO (contact)

PROGRAM_OFFICER_NAME: BERNAL, FEDERICO

PROJECT_TERMS: addiction;Advisory Committees;Alcohols;Anatomy;Animals;Applications Grants;Area;Award;Basic Science;Benchmarking;Biology;Biomedical Research;biomedical resource;Businesses;career;Centers of Research Excellence;Chemistry;Clinical Research;Collaborations;Communities;Contract Services;Core Facility;Cyclic GMP;design and construction;Development;Disease;Educational workshop;Electrophysiology (science);Ensure;Entrepreneurship;experience;Faculty;Familiarity;Fees;Floor;Fluorescence;Fostering;Funding;Goals;Grant;IACUC;Image;Industry;industry partner;innovation;Institution;instrument;instrumentation;Interdisciplinary Study;Investigation;Investments;Leadership;Medical;medical schools;member;Mental disorders;Mentors;Mentorship;Microscope;Microscopy;Molecular;multidisciplinary;multiphoton microscopy;Nerve Degeneration;Nervous System Disorder;neural;neuroadaptation;Neurobiology;neuroimaging;Neuronal Plasticity;neuroprotection;Neurosciences;Participant;patch clamp;Pharmacology;Pharmacy facility;Phase;Physiology;Pilot Projects;pre-clinical research;preclinical study;Principal Investigator;Privatization;Productivity;programs;Puerto Rico;Qualifying;recruit;Research;Research Infrastructure;Research Personnel;Research Project Grants;Resources;Science;Scientist;Services;Slide;Source;square foot;synergism;Therapeutic;Training;translational applications;United States National Institutes of Health;Universities

PROJECT_TITLE: COBRE PHASE III:  Center for Neuroplasticity at the University of Puerto Rico


NIH_SPENDING_CATS: Acquired Cognitive Impairment;Aging;Alzheimer's Disease;Alzheimer's Disease including Alzheimer's Disease Related Dementias (AD/ADRD);Bioengineering;Brain Disorders;Dementia;Neurodegenerative

ORG_NAME: UNIVERSITY OF MISSOURI-ST. LOUIS

PHR: PROJECT NARRATIVE The instrument requested in this Administrative Supplement for Equipment Purchase is a Tecan Spark Cyto 400 multimode cell imaging plate reader, which will significantly enhance the research aims of the parent grant award (1R15GM152964-01) and the research environment at the University of Missouri-St. Louis. The parent award project is investigating the function of a group of proteins found inside of immune cells. The research has implications for a variety of human health problems related to inflammation including neurodegenerative and cardiovascular. The instrument possesses substantial versatility and will improve the speed and quality of the parent NIH project findings.

PI_NAMEs: NICHOLS, MICHAEL R. (contact)

PROGRAM_OFFICER_NAME: ZHAO, XIAOLI

PROJECT_TERMS: 20 year old;Administrative Supplement;Amyloid beta-Protein;Award;Biological Assay;Cardiovascular system;Cells;cellular imaging;Environment;equipment acquisition;Fluorescence;Health;Human;imaging capabilities;Immune;improved;Inflammasome;Inflammation;Institution;instrument;Lead;live cell imaging;luminescence;Mechanics;Missouri;Nerve Degeneration;parent grant;parent project;Parents;Phase;Proteins;Reader;Research;Research Personnel;Speed;Students;Training;undergraduate student;United States National Institutes of Health;Universities

PROJECT_TITLE: Mechanisms of NLRP3 inflammasome activation


ED_INST_TYPE: EARTH SCIENCES/RESOURCES

NIH_SPENDING_CATS: Infectious Diseases

PHR: PROJECT NARRATIVE: Targeting the molecular interactions of structural exopolysaccharides in bacterial biofilms is a promising new approach to treat persistent and hospital acquired infections resulting from biofilm infections. This proposed research will develop new tools to identify critical protein- exopolysaccharide binding interactions that occur in live bacterial biofilms and will also identify novel biocatalyst capable of breaking down biofilm exopolysaccharides through functional metagenomic screening and through protein engineering to improve the catalytic activity of existing biofilm dispersal agents.

PI_NAMEs: POULIN, MYLES B (contact)

PROGRAM_OFFICER_NAME: BOND, MICHELLE RUEFFER

PROJECT_TERMS: analog;Antibiotics;Area;Bacterial Infections;Binding;Binding Proteins;biochemical tools;carbohydrate binding protein;carbohydrate receptor;Cell Communication;Cells;Chemicals;Engineering;Enzymes;Glycoside Hydrolases;Goals;Healthcare;Human;Immune system;improved;Infection;insight;Label;Laboratories;metagenome;Metagenomics;Microbial Biofilms;Molecular Target;Mutagenesis;Nosocomial Infections;novel;novel strategies;Protein Engineering;Proteins;Research;Role;screening;Site;Therapeutic;Therapeutic Uses;tool

PROJECT_TITLE: Chemical and biochemical tools to study the functions of exopolysaccharides in bacterial biofilms


NIH_SPENDING_CATS: Behavioral and Social Science;Building Research Capacity at Institutions with Limited NIH Funding;Clinical Research;Genetics;Networking and Information Technology R&D (NITRD)

ORG_CITY: VERMILLION

ORG_DEPT: OTHER BASIC SCIENCES

ORG_NAME: UNIVERSITY OF SOUTH DAKOTA

ORG_STATE: SD

PHR: Project Narrative Leveraging phylogenetic analysis use cases provides substantial value, granting researchers a deeper understanding of the evolutionary progression of species and their connections. In this context, we introduce a cloud-based phylogenetic analysis training framework that is designed to encompass a generalized workflow. Our primary focus will be directed toward specific use cases intricately tailored to meet the needs of our user community within our cloud-based learning modules.

PI_NAMEs: HUBER, VICTOR CHESTER (contact)

PROJECT_TERMS: Address;Bacteria;base;bioinformatics tool;Biological;biological research;Biological Sciences;Characteristics;cloud based;Cloud Computing;Communities;Community Health Education;Complex;Comprehension;Computer software;computing resources;Data;Data Set;design;Development;Disease;Ecology;Ensure;Epidemiology;Evolution;Exercise;Fluorescence;functional genomics;gene function;Genomics;Grant;Health;Human;Immune;insight;Instruction;Investigation;Knowledge;Learning;Learning Module;Malignant Neoplasms;Metabolic Pathway;Microbial Biofilms;Molecular;Molecular Biology;Morphology;novel;Organism;pathogen;Pattern;Phylogenetic Analysis;Phylogeny;Play;Prevalence;Proteins;Research;Research Personnel;Resources;Role;Sampling;Science;skills;Software Validation;Spatial Distribution;Specific qualifier value;Students;Technology;tool;Toy;Training;trait;transmission process;Trees;whole genome

PROJECT_TITLE: Learning phylogenetic tree design and analysis with application


ORG_CITY: PORTLAND

ORG_NAME: MAINEHEALTH

ORG_STATE: ME

PHR: Narrative The COBRE in Acute Care Research & Rural Disparities at Maine Medical Center is seeking to upgrade the aging flow cytometer within the MaineHealth Institute for Research Flow Cytometry Core to the next-generation multicolor flow cytometer, crucial for advancing our research efforts. This upgrade is vital for achieving our objectives of mentoring acute care researchers, strengthening research infrastructure, and advancing our long- term goal of improving acute care outcomes for patients in rural areas.

PI_NAMEs: SAWYER, DOUGLAS B (contact)

PROJECT_TERMS: acute care;Aging;Antibiotics;Area;biomarker panel;Caring;Cells;Centers of Research Excellence;Color;Communities;Contract Services;Core Facility;cost;discount;Equipment;Flow Cytometry;Funding;Future;Goals;Immune;improved;Institution;instrument;Investments;Life;Maine;Medical center;Mentors;Molecular;Newborn Infant;next generation;parent grant;Patient-Focused Outcomes;Phase;Play;programs;prophylactic;Research;Research Infrastructure;Research Institute;Research Personnel;Research Project Grants;Resuscitation;Role;rural area;rural disparities;Scientific Advances and Accomplishments;Services;Signal Transduction;stem cells;success;System;Telemedicine;Wages

PROJECT_TITLE: Center of Biomedical Research Excellence in Acute Care Research and Rural Disparities - Equipment


ED_INST_TYPE: UNIVERSITY-WIDE

ORG_CITY: DENVER

ORG_NAME: UNIVERSITY OF DENVER (COLORADO SEMINARY)

ORG_STATE: CO

PHR: PROJECT NARRATIVE Carbon monoxide (CO) gas has been identified as an essential signaling molecule in the human neuronal system and has been shown to have many therapeutic applications. Molecules that create CO gas upon activation with an external stimulus are commonly dubbed CO releasing molecules, or CORMs, and have shown to be vital tool in studying the effect of CO in biological systems. This research proposes to create a new class of CORMs based on organic molecules that efficiently produce CO gas, are directly polymerizable, and have predictable and well-defined products following the release of CO, attributes not currently accessible with state-of-the-art CORMs.

PI_NAMEs: WORRELL, BRADY  (contact)

PROGRAM_OFFICER_NAME: ASLAN, KADIR

PROJECT_TERMS: Acute;analog;biological systems;biomaterial compatibility;Carbon Monoxide;Cardiovascular Diseases;Chronic;Collaborations;Colorado;Complex;cytotoxic;Development;Future;Gases;gastrointestinal;Health;Human;Human body;improved;Inflammation;Laboratories;learning materials;Ligation;medical schools;member;metal complex;method development;Neurons;new therapeutic target;organ transplant rejection;Pathway interactions;photolysis;polymerization;Polymers;Production;programs;Research;response;Signaling Molecule;small molecule;Solubility;Stimulus;System;targeted treatment;Technology;Therapeutic;tool;Transition Elements;Universities;Water;Work

PROJECT_TITLE: CO Releasing Organic Polymers for Biomedical Applications


ADMINISTERING_IC: IP

IC_NAME: NATIONAL CENTER FOR IMMUNIZATION AND RESPIRATORY DISEASES

ORG_CITY: SCOTTSDALE

ORG_NAME: VITALANT

ORG_STATE: AZ

PHR: PROJECT NARRATIVE  Monitoring the burden of respiratory virus infections and disease is essential to public health surveillance and response, and foundational to preparedness for novel viral agents with pandemic potential. In this program, antibody test results and survey responses obtained from a large cohort of blood donors will be applied to calculate estimates of symptomatic and asymptomatic infections caused by major respiratory viruses nationally, within U.S. regions, and in specific race/ethnicity, sex, and age subgroups. Data generated in this program will also be used to investigate risk factors for infections; estimate antibody levels that protect against infections (including from new viral variants); and monitor uptake and attitudes towards vaccines, other prevention measures, and therapeutics.

PI_NAMEs: BUSCH, MICHAEL PAUL (contact)

PROJECT_TITLE: RFA-IP-24-046: Nationwide Cohort of Blood Donors to Estimate Burden of Respiratory Viruses and Immunologic Response

STUDY_SECTION_NAME: Special Emphasis Panel[ZIP1 GCA (62)]


ADMINISTERING_IC: AA

ED_INST_TYPE: SCHOOLS OF PUBLIC HEALTH

IC_NAME: NATIONAL INSTITUTE ON ALCOHOL ABUSE AND ALCOHOLISM

NIH_SPENDING_CATS: Aging;Alcoholism, Alcohol Use and Health;Basic Behavioral and Social Science;Behavioral and Social Science;Osteoporosis;Physical Injury - Accidents and Adverse Effects;Social Determinants of Health;Substance Misuse;Women's Health Research

ORG_CITY: CORVALLIS

ORG_NAME: OREGON STATE UNIVERSITY

ORG_STATE: OR

PHR: PROJECT NARRATIVE Chronic heavy alcohol consumption has been implicated in having negative effects on the skeleton. The proposed studies will evaluate the impact of heavy alcohol consumption on bone in a non-human primate model for voluntary drinking and in humans with chronic alcohol abuse who died following an accident. This research is important because of the large economic, social, and personal burden associated with poor bone health.

PI_NAMEs: IWANIEC, URSZULA T (contact)

PROGRAM_OFFICER_NAME: LIN, LI

PROJECT_TERMS: Accidents;Age;Alcohol abuse;alcohol abuser;alcohol availability;alcohol comorbidity;Alcohol consumption;alcohol effect;alcohol use disorder;Alcohols;Animal Model;Architecture;Biopsy;bone;Bone Density;bone health;bone loss;bone mass;bone quality;Bone remodeling;bone turnover;Chronic;chronic alcohol ingestion;comorbidity;Complex;cortical bone;density;Dose;drinking;drinking behavior;Economics;Elderly;Equilibrium;Exhibits;Fatigue;Female;Fracture;Funding;Goals;Heavy Drinking;Human;human female;human male;Individual;Intervention;Libraries;Life Style;lifestyle factors;lumbar vertebra bone structure;Macaca;Macaca mulatta;Machine Learning;male;Mediating;men;middle age;Modeling;Monkeys;Morbidity - disease rate;mortality;nonhuman primate;Organ;Osteopenia;Physiology;Population;Porosity;prevent;Process;R24;repaired;Research;Resources;response;Risk Factors;sex;Site;skeletal;Skeleton;social;substantia spongiosa;Sudden Death;Systems Analysis;Testing;tibia;tissue archive;Tissues;Trauma;United States National Institutes of Health;young adult

PROJECT_TITLE: Effects of alcohol on bone remodeling balance in male and female non-human primates and humans

STUDY_SECTION: MRS

STUDY_SECTION_NAME: Musculoskeletal Rehabilitation Sciences Study Section[MRS]


ORG_CITY: BALTIMORE

ORG_DEPT: ANATOMY/CELL BIOLOGY

ORG_NAME: JOHNS HOPKINS UNIVERSITY

PHR: Project Narrative Relevance to Public Health Due to its significant involvement in biological processes, chemotaxis contributes to disease progression in cancer and arthritis, while its impairment leads to anomalous tissue development or regeneration. Our unique molecular actuators that can exert “physical force” specifically onto intended intracellular objects, can be a tool for not only dissecting the molecular mechanisms of chemotaxis, but also interfering with these cell migration-related diseases.

PI_NAMEs: INOUE, TAKANARI  (contact)

PROJECT_TERMS: Actins;angiogenesis;Arthritis;Back;Biochemical Reaction;Biological Process;Cell membrane;cell motility;Characteristics;Chemotactic Factors;Chemotaxis;Cytoskeleton;Development;Disease;Disease Progression;Embryonic Development;Event;Exhibits;Feedback;Filopodia;Goals;Impairment;Link;Logic;loss of function;Malignant Neoplasms;Mechanics;Membrane;Molecular;molecular actuator;Monomeric GTP-Binding Proteins;Morphology;Natural regeneration;Neoplasm Metastasis;Nuclear Envelope;operation;Pathologic;Phase;Phenotype;Physical condensation;physical property;Physiological;PIK3CG gene;polarized cell;Property;Proteins;Public Health;Receptor Protein-Tyrosine Kinases;rho GTP-Binding Proteins;Series;Signal Transduction;spatiotemporal;Techniques;Tissues;tool;transcription factor;wound healing

PROJECT_TITLE: Decoding dynamic interplay between signaling and membranes in chemotaxis by molecular actuators


NIH_SPENDING_CATS: Estrogen;Genetics;Human Genome;Stem Cell Research;Stem Cell Research - Embryonic - Non-Human;Women's Health Research

ORG_CITY: ANN ARBOR

ORG_NAME: UNIVERSITY OF MICHIGAN AT ANN ARBOR

ORG_STATE: MI

PHR: Narrative This proposal is relevant to public health because it seeks to understand how the X chromosome-encoded KDM5C protein regulates gene expression genome-wide in a sexually-dimorphic manner, including investigating how females with two X chromosomes and males with a single X chromosome equalize gene expression from the X chromosome, independently of gonadal hormones. The proposal is relevant to the part of the NIH mission that seeks to advance fundamental knowledge about the nature of living systems.

PI_NAMEs: KALANTRY, SUNDEEP  (contact)

PROGRAM_OFFICER_NAME: CHOW, CHI-WING

PROJECT_TERMS: Ablation;autosome;Cells;Chromatin;Data;demethylation;Dissection;Dose;Embryo;embryo cell;embryonic stem cell;Epigenetic Process;Female;Funding;Gene Expression;Gene Expression Profiling;Genes;Genetic;Genetic Transcription;genome-wide;Goals;Gonadal Hormones;Histones;Hormones;innovation;Knowledge;Link;male;Mammals;Mission;Modification;Mus;Nature;novel;Organism;prevent;Proteins;Public Health;Regulation;Research;sex;Sex Bias;Sex Differences;sexual dimorphism;Source;stem cells;Technology;Testing;Transcriptional Regulation;United States National Institutes of Health;Work;X Chromosome;X Inactivation;Y Chromosome

PROJECT_TITLE: X-chromosome Inactivation Catalyzed by Genes that Escape X-inactivation


NIH_SPENDING_CATS: Alcoholism, Alcohol Use and Health;Breast Cancer;Cancer;Endocrine Disruptors;Pediatric;Perinatal Period - Conditions Originating in Perinatal Period;Prevention;Stem Cell Research;Stem Cell Research - Nonembryonic - Non-Human;Substance Misuse;Women's Health Research

ORG_CITY: DURHAM

ORG_NAME: NORTH CAROLINA CENTRAL UNIVERSITY

PHR: PROJECT NARRATIVE We apply for the administrative supplement fund to purchase a benchtop cell sorter to enhance the accomplishment of the parental R16 grant. The parental project aims to investigate the mechanisms of in utero exposure to alcohol on mammary tumor development in MMTV-erbB2 transgenic mice. We will focus on in utero exposure to alcohol-induced estrogen receptor-erbB2 crosstalk in signal transduction and mammary stem cell reprogramming. The benchtop cell sorter will enhance a successful completion of the project.

PI_NAMEs: YANG, XIAOHE  (contact)

PROGRAM_OFFICER_NAME: REVILLEZA, MARIA

PROJECT_TERMS: Administrative Supplement;alcohol exposure;Biological Models;Breast Cancer Risk Factor;Breast Epithelial Cells;Cancer Etiology;cancer prevention;cancer stem cell;Carcinogens;Cell Differentiation process;Cell Reprogramming;Cells;clinically relevant;Data;Development;disorder risk;Environmental Risk Factor;erbB-2 Receptor;ERBB2 gene;ERBB2 Signaling Pathway;Estrogen Receptors;estrogenic;Exposure to;Fetal Development;Fetal Diseases;Funding;Gene Expression;Goals;Grant;Health;Life;Link;Malignant Breast Neoplasm;mammary;Mammary gland;Mammary Neoplasms;maternal alcohol use;Microarray Analysis;Modeling;Mouse Mammary Tumor Virus;mouse model;Mus;Myoepithelial;novel strategies;Pathway interactions;Pregnancy;premalignant;prenatal exposure;Prevention strategy;Puberty;Receptor Protein-Tyrosine Kinases;response;Risk;Role;Signal Pathway;Signal Transduction;stem cells;Tissues;Transgenic Mice;tumor;tumor initiation;Up-Regulation

PROJECT_TITLE: In utero exposure to alcohol-induced mammary stem cell deregulation and tumor risk later in life.


NIH_SPENDING_CATS: Brain Disorders;Genetics;Muscular Dystrophy;Myotonic Dystrophy;Neurodegenerative;Neurosciences;Rare Diseases

PHR: Public health relevance Messenger RNAs (mRNAs) transfer genetic information from the nucleus to the cytoplasm where they are translated. Establishment of the fly and frog lineages that provide continuity of the species and mammalian synaptic activity rely critically on mRNA localization. However, in humans, mislocalized mRNAs cause muscle disorders such as myotonic dystrophy 1 and have been linked to neurodegeneration, including amyotrophic lateral sclerosis. Our findings in this study will elucidate how organisms harness spatially organized mRNAs to promote development. Importantly, this knowledge will also enable fundamental understanding of how misregulation of mRNA localization could contribute to diseases such as MD1, ALS, and SCA.

PI_NAMEs: TRCEK, TATJANA  (contact)

PROJECT_TERMS: Acute;Amyotrophic Lateral Sclerosis;Automobile Driving;Base Pairing;Biological Process;Cell Lineage;Cell Nucleus;Cells;Cellular Morphology;Cytoplasm;Cytoplasmic Granules;Data;design;Development;Disease;Drosophila genus;Environment;fly;Genetic;genetic information;Germ;Goals;Human;human disease;imaging approach;In Vitro;in vivo;insight;Knowledge;Light;Link;Membrane;Messenger RNA;Methods;Myopathy;Myotonic Dystrophy;Nerve Degeneration;Oils;Organism;particle;Pathogenesis;Phase;prevent;Process;Property;public health relevance;Rana;recruit;Resolution;Ribonucleoproteins;RNA;RNA Helicase;RNA-Binding Proteins;Role;self assembly;self organization;Specific qualifier value;Spinocerebellar Ataxias;Structure;Subcellular structure;superresolution imaging;Synapses;Transfer RNA;Translating;Translations;Water

PROJECT_TITLE: mRNA assembly in Drosophila germ granules


ED_INST_TYPE: GRADUATE SCHOOLS

ORG_CITY: NEW YORK

ORG_NAME: COLUMBIA UNIV NEW YORK MORNINGSIDE

PHR: Principal Investigator/Program Director (Last, First, Middle): Rovis, Tomislav  Selective Functionalization of Aliphatic Amines – Supplement Request Project Narrative This is a Supplement Request to replace a key piece of equipment that facilitates our NIGMS funded research program entitled “Selective Functionalization of Aliphatic Amines”. Advances in health related research need specialized equipment to accelerate the pace of discovery. Equipment that is heavily used has a finite lifetime. We seek funds to replace one such key piece of equipment. PHS 398/2590 (Rev. 09/04) Page Continuation Format Page

PI_NAMEs: ROVIS, TOMISLAV  (contact)

PROGRAM_OFFICER_NAME: YANG, JIONG

PROJECT_TERMS: Acceleration;Age;Amines;Atmosphere;Equipment;Funding;Health;Investigation;National Institute of General Medical Sciences;Principal Investigator;programs;Reaction;Research;Solvents;Work

STUDY_SECTION: SBCA

STUDY_SECTION_NAME: Synthetic and Biological Chemistry A Study Section[SBCA]


ORG_CITY: WILMINGTON

ORG_NAME: NEMOURS CHILDREN'S HOSPITAL, DELAWARE

PHR: PROJECT NARRATIVE The REACH (Research Expanding Access to Child Health) Center of Biomedical Research Excellence is providing the infrastructure needed for researchers across Delaware to join with stakeholders and community partners in the development, evaluation, and implementation of innovative interventions and models of care to overcome the complex socio-ecological factors responsible for pediatric health disparities. This Administrative Supplement to Recognize Excellence in Diversity, Equity, Inclusion, and Accessibility (DEIA) Mentorship will allow us to expand our REACH Center mentorship program to support a broader and more diverse group of investigators, staff, and trainees, cultivating their skills, benefiting the quality and impact of our research, and building a diverse biomedical research workforce. With this additional support, the REACH Center will propel scientific knowledge, technical capabilities, and clinical practice forward to improve healthcare for all children and families across Delaware with broad implications nationally.

PI_NAMEs: ALDERFER, MELISSA A.;KAZAK, ANNE E (contact)

PROGRAM_OFFICER_NAME: PEI, WUHONG

PROJECT_TERMS: Address;Administrative Supplement;Award;Awareness;Biomedical Research;career;Career Choice;career development;Caring;Centers of Research Excellence;Child;Child Health;Child health care;Childhood;Clinical;clinical practice;cohort;Communities;community partners;Complex;Dedications;Delaware;Development;disadvantaged background;Doctor of Philosophy;effective intervention;Eligibility Determination;Ensure;Environment;Equity;equity, diversity, and inclusion;Evaluation;evidence base;experience;Family;Fostering;Funding;Generations;Goals;Health;health care delivery;health disparity;health equity;Health equity research;health inequalities;Healthcare;Historically Black Colleges and Universities;improved;Individual;Inequity;Infrastructure;innovation;Institution;interest;Internships;Intervention;Knowledge;Lead;Leadership;member;Mentors;Mentorship;Methods;Modeling;multidisciplinary;Pathway interactions;Pediatric Hospitals;Pediatric Research;Pediatrics;Personal Satisfaction;Pilot Projects;Population;Postdoctoral Fellow;Process;professor;programs;Recommendation;Reduce health disparities;Research;Research Infrastructure;Research Personnel;Research Project Grants;Research Proposals;Research Support;Research Training;Resources;Scientist;skills;social;success;Technology;Testing;theories;Time;Training;Underrepresented Populations;Underserved Population;United States;Universities;Woman

PROJECT_TITLE: Research Expanding Access to Child Health (REACH)


NIH_SPENDING_CATS: Arthritis

PHR: Project Narrative The activation of dioxygen by heme and nonheme metalloenzymes plays a critical role in many fundamental biological processes, including biosynthesis, detoxification, metabolic processes, and energy transfer, all of which directly or indirectly impact human health. The proposed studies focus on the synthesis and reactivity of small-molecule analogs of metalloenzyme active sites that process and manipulate various forms of oxygen. These efforts will provide fundamental knowledge relating to enzyme mechanisms which are implicated in a variety of diseases, including arthritis, cancer, genetic and neurodegenerative disorders.

PI_NAMEs: GOLDBERG, DAVID P (contact)

PROJECT_TERMS: Active Sites;Address;adduct;Anabolism;analog;Arthritis;Biological Process;Biomimetics;Chemistry;Complex;Cysteine dioxygenase;Cytochrome P450;Diagnostic;Dioxygen;Dioxygenases;Disease;Drug Metabolic Detoxication;electronic structure;Electronics;Encephalopathies;Energy Transfer;enzyme mechanism;Enzymes;Event;Genetic Diseases;Health;Heme;Human;Hydrogen Bonding;Hydroxylation;indoleamine;Indoles;Iron;isopenicillin N;Kinetics;Knowledge;Ligands;Malignant Neoplasms;Mediating;Metabolism;metal complex;metalloenzyme;Metals;Methodology;Mixed Function Oxygenases;Modification;Molecular Weight;Neurodegenerative Disorders;oxidation;Oxygen;Pathway interactions;persulfides;Play;Process;Property;Proteins;Role;small molecule;Structure-Activity Relationship;Sulfhydryl Compounds;Sulfoxide;Sulfur;System;Therapeutic;Thermodynamics;Transition Elements;Tryptophan

PROJECT_TITLE: Heme and Nonheme Transition Metal Complexes, Reactivity, and Mechanism


NIH_SPENDING_CATS: Bioengineering;Cancer;Digestive Diseases;Kidney Disease;Liver Disease;Obesity

ORG_CITY: OMAHA

ORG_NAME: UNIVERSITY OF NEBRASKA MEDICAL CENTER

ORG_STATE: NE

PHR: Project Narrative This project is addresses basic mechanisms that mediate lipid droplet catabolism and biogenesis, both of which are critical for fundamental cellular processes. Because LD catabolism is dysregulated in multiple diseases including fatty liver, obesity, diabetes, kidney disease and cancer, elucidating these mechanisms creates an opportunity to identify targets in LD catabolism that and improve human health.

PI_NAMEs: SCHOTT, MICAH  (contact)

PROGRAM_OFFICER_NAME: NIE, ZHONGZHEN

PROJECT_TITLE: Mechanisms of endosomal trafficking in lipid droplet catabolism


ORG_CITY: ROCHESTER

ORG_NAME: ROCHESTER INSTITUTE OF TECHNOLOGY

ORG_STATE: NY

PHR: Project Narrative To expand our knowledge about the cues that promote directed cell migration, we propose a new experimental platform that combines aligned collagen fiber landscapes with soluble biochemical gradients to study directed migration within a complex 3D environment. If successful, our platform will dissect how different cell populations prioritize and respond to simultaneous guidance cues with relevance to early development, immune cell trafficking, vascularization, and cancer invasion.

PI_NAMEs: ABHYANKAR, VINAY V (contact)

PROGRAM_OFFICER_NAME: RIGAS, MARC

PROJECT_TITLE: Directed Cell Motility Along Gradients in Extracellular Matrix Fiber Alignment


ORG_CITY: Chicago

ORG_NAME: UNIVERSITY OF ILLINOIS AT CHICAGO

ORG_STATE: IL

PHR: WONHWA CHO, PH.D. PUBLIC HEALTH RELEVANCE STATEMENT Lipid signaling is an important target for drug development for diverse human diseases, including cancer. Based on our recent research showing that membrane lipids directly and specifically control cellular activities of diverse proteins that are critically involved in cancer, we propose to elucidate the mechanisms underlying complex lipid-mediated cell regulation and develop drugs targeting lipid- protein binding interfaces. Given the general and fundamental importance of lipid signaling, our pioneering, innovative research should have major impact on broad areas of biology and medicine.

PI_NAMEs: CHO, WONHWA  (contact)

PROJECT_TERMS: Affinity;Area;Binding;Binding Proteins;Biology;cell growth regulation;Cell Physiology;Cells;Cholesterol;Complex;Disease;DLG4 gene;Doctor of Philosophy;drug development;drug discovery;Drug Targeting;Foundations;Health;human disease;Human Genome;Imaging technology;In Situ;innovation;Lipids;Malignant Neoplasms;Mediating;Medicine;Membrane Lipids;Phosphatidylinositols;Physiological;Process;protein complex;protein protein interaction;Proteins;public health relevance;Regulation;Research;Signal Pathway;Signal Transduction;spatiotemporal;Specificity

PROJECT_TITLE: Lipid regulation of cellular signaling and protein-protein interactions


ORG_NAME: NEW YORK UNIVERSITY

PHR: Project Narrative - From Initial Application This proposal describes the development of chemical and biochemical tools to probe the incorporation of bacterial sugars into the cell surface. In doing so, we will provide a better understanding of bacterial cell envelope assembly, and the molecular recognition of unique (rare) bacterial sugars during host-pathogen interactions. The outcome of this work has applications in the development of long sought-after antibiotics to treat Gram- negative bacterial infections, and will provide a fundamental understanding of bacterial glycosyltransferase function.

PI_NAMEs: LUPOLI, TANIA  (contact)

PROJECT_TERMS: Antibiotics;Area;Bacteria;Bacterial Polysaccharides;Bacterial Proteins;Bar Codes;biochemical tools;Biochemistry;Biology;Cause of Death;cell envelope;Cell surface;Chemicals;Development;Educational process of instructing;Environment;Epitopes;glycosyltransferase;Gram-Negative Bacteria;Gram-Negative Bacterial Infections;Human;improved;Infection;insight;interdisciplinary approach;Label;Laboratories;Mediating;Membrane;Microbiology;Molecular;Molecular Chaperones;molecular recognition;Monosaccharides;novel diagnostics;novel strategies;O Antigens;Oligosaccharides;Organic Chemistry;Organism;Outcome;pathogen;pathogenic bacteria;Pathway interactions;Pattern;Planets;Polymers;Polysaccharides;Predisposition;Production;programs;protein protein interaction;Proteins;Public Health;Reagent;Route;Skin;small molecule;Structure;sugar;Surface;symbiont;United States National Institutes of Health;Work

PROJECT_TITLE: Probing Glycan Polymer Patterns on Bacterial Cell Surfaces


ED_INST_TYPE: ORGANIZED RESEARCH UNITS

NIH_SPENDING_CATS: Biotechnology;Emerging Infectious Diseases;Genetics;Infectious Diseases

ORG_CITY: STONY BROOK

ORG_DEPT: MISCELLANEOUS

ORG_NAME: STATE UNIVERSITY NEW YORK STONY BROOK

PHR: Narrative: Administrative Supplement to NIGMS MIRA R35GM122561 Parent project title: Dynamics and evolution of synthetic and natural gene regulatory networks This Administrative Supplement is a request for support for a Jess automated capillary-based western blot system by Bio-Techne, which allows for rapid detection of up to a dozen proteins in a single sample in a quantitative, highly reproducible manner, without a need for genetic modification. The Jess system will enable quantitative measurements of how synthetic biological control of genes affects protein levels, to be linked with cellular phenotypes. This will remove a bottleneck by providing information rapidly on multiple protein levels that can be further investigated to understand the network-level effects of synthetic biological perturbations, as proposed in the parent project.

PI_NAMEs: BALAZSI, GABOR  (contact)

PROGRAM_OFFICER_NAME: YU, GUOQIN

PROJECT_TERMS: Administrative Supplement;Affect;Award;Behavior;Biological;Biology;Blood capillaries;cancer drug resistance;Cells;Computer Models;equipment acquisition;Evolution;Funding;Gene Expression Profile;gene network;gene regulatory network;Genes;Genetic;interest;Learning;Link;Measurement;Microbial Drug Resistance;Modification;multiplex detection;National Institute of General Medical Sciences;parent project;Phenotype;Population;Proteins;rapid detection;Reproducibility;RNA;Sampling;Signal Transduction;Synthetic Genes;System;Time;Western Blotting


NIH_SPENDING_CATS: Bioengineering;Nutrition;Prevention

ORG_CITY: MOUNT PLEASANT

ORG_NAME: CENTRAL MICHIGAN UNIVERSITY

PHR: Public Health Relevance Statement (Narrative attachment) We will synthesize safe, powerful and hydrophilic/amphiphilic antioxidant dendrimers that would be beneficial for the prevention and treatment of diseases that have a strong association with oxidative stress (chronic inflammation), such as cancer, diabetes, asthma, atherosclerosis, arthritis, and neurodegenerative diseases. The synthesized hydrophilic/amphiphilic super antioxidants in a dendritic architecture will be tested to investigate their protective effects on biomolecules and anti-inflammatory action on cells against free radicals.

PI_NAMEs: LEE, CHOON YOUNG (contact)

PROGRAM_OFFICER_NAME: ANSONG, CHARLES KWAKU

PROJECT_TERMS: 14 year old;Acceleration;Administrative Supplement;Age;Alkynes;amphiphilicity;Anti-Inflammatory Agents;Antioxidants;Architecture;Arthritis;Asthma;Atherosclerosis;Azides;biomaterial compatibility;catalyst;Cells;chemical reaction;Chemistry;Chronic;Communities;Complex;Copper;Copper Chelation;Cytoplasmic Granules;Dendrimers;Deterioration;Diabetes Mellitus;Disease;Engineering;Free Radicals;Funding;Grant;Heating;Hour;hydrophilicity;Inflammation;Ions;Malignant Neoplasms;Metals;Michigan;microwave electromagnetic radiation;Modernization;Neurodegenerative Disorders;Oxidative Stress;pressure;Prevention;protective effect;public health relevance;rat Ran 2 protein;Reaction;reaction rate;Reactive Oxygen Species;Research;Research Design;scaffold;Science;Solubility;Solvents;Students;Techniques;Temperature;Testing;Time;Training;Transition Elements;undergraduate student;Universities;Vitamin B6

PROJECT_TITLE: Engineering hydrophilic/amphiphilic Vitamin B6-based super antioxidant dendrimers for  controlling chronic inflammation


ORG_CITY: GREENVILLE

ORG_NAME: FURMAN UNIVERSITY

ORG_STATE: SC

PHR: PROJECT NARRATIVE Desmosomes are protein complexes required for strong connections between cells, maintenance of tissue structure, and other cellular functions (such as proliferation and migration) via cell signaling. In our study, we propose to study how the desmosomal protein Desmoglein-2 controls the ability of cells to attach to and migrate on the extracellular matrix, a network of proteins which surrounds cells within tissues. These studies will provide important insights into how the attachments between cells control their migratory behavior, which is important during processes such as wound healing and tissue repair.

PI_NAMEs: DUBASH, ADI D (contact)

PROJECT_TERMS: Adhesives;Apoptosis;Behavior;Binding;Biological;Biological Process;Cadherins;career;Cell membrane;cell motility;Cell Physiology;Cell-Cell Adhesion;Cell-Matrix Junction;Cells;Collagen;Complex;Data;desmoglein 2;desmoplakin;Desmosomes;electric impedance;experimental study;extracellular;Extracellular Domain;Extracellular Matrix;Extracellular Matrix Proteins;Fibronectins;Fostering;Foundational Skills;Gene Expression;Guanosine Triphosphate Phosphohydrolases;insight;Institution;knock-down;Knock-out;Maintenance;Mechanical Stress;Mediating;Methods;migration;Molecular;Morphogenesis;NF-kappa B;novel;Play;Process;Proliferating;protein complex;Proteins;re-epithelialization;Regulation;response;Role;Science;Signal Transduction;Structure;Testing;tissue repair;Tissues;Training;undergraduate student;Universities;Work;wound healing

PROJECT_TITLE: Analysis of Desmoglein 2-mediated control of cell spreading and migration by cellular impedance.


NIH_SPENDING_CATS: Basic Behavioral and Social Science;Behavioral and Social Science;Brain Disorders;Mental Health;Mental Illness;Neurosciences

ORG_STATE: MD

PHR: Narrative Many animals perform complex behaviors that are the result of varying arousal/motivational states, though identifying these states by behavior alone is challenging. In turn, how these states influence specific neuronal circuits to alter behavior is diffifcult in complex brains, but tractable in smaller invertebrate brains. We propose to use orb-weaving spiders to investigate how neuromodulation organizes behavioral states, and nematodes to understand how these states alter neuronal architecture.

PI_NAMEs: GORDUS, ANDREW GEORGE (contact)

PROGRAM_OFFICER_NAME: STEPANEK, LAURIE ELIZABETH

PROJECT_TITLE: The influence of neuronal states on perception and behavior


NIH_SPENDING_CATS: Antimicrobial Resistance;Biodefense and Related Countermeasures;Bioengineering;Emerging Infectious Diseases;Infectious Diseases;Nanotechnology

ORG_CITY: CHARLOTTE

ORG_NAME: UNIVERSITY OF NORTH CAROLINA CHARLOTTE

ORG_STATE: NC

PHR: Project Narrative Antimicrobial resistant bacteria (ARB) and genes (ARGs) are one of the biggest public health issues of the 21st century. In this project, we propose to develop a light-activated silver nanoparticulate system for the effective treatment of ARB and ARGs. The proposed research is relevant to public health because it will develop reliable technologies for the effective elimination of ARB and ARGs.

PI_NAMEs: VIVERO-ESCOTO, JUAN LUIS (contact)

PROGRAM_OFFICER_NAME: CUPIT, PAULINE

PROJECT_TERMS: anti-microbial drug;Antibiotic Resistance;antibiotic resistant infections;Antibiotics;antimicrobial;Antimicrobial Resistance;Area;Bacteria;Bacterial Antibiotic Resistance;bacterial resistance;Cells;Chemicals;cytotoxicity;daughter cell;Development;DNA;effective therapy;efflux pump;Environment;Formulation;Generations;Genes;Goals;Horizontal Gene Transfer;Ions;irradiation;Kinetics;Light;Mammalian Cell;microbial;Modality;Mutation;nanomaterials;nanoparticle;nanoparticulate;pathogen;Pathway interactions;Persons;protoporphyrin IX;Public Health;Pump;rational design;Reporting;Research;Resistance;Resistance development;resistance gene;resistance mechanism;Silver;success;Surface;Surface Properties;System;Technology;Therapeutic

PROJECT_TITLE: Light-Activated Silver Nanoparticles to Eliminate Antibiotic Resistant Bacteria and Genes


ADMINISTERING_IC: ES

ED_INST_TYPE: SCHOOL OF MEDICINE & DENTISTRY

IC_NAME: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES

NIH_SPENDING_CATS: Clinical Research;Prevention;Social Determinants of Health

ORG_DEPT: PUBLIC HEALTH & PREV MEDICINE

ORG_NAME: UNIVERSITY OF ROCHESTER

PHR: Project Narrative The mission of the Rochester EHSC is to improve health through the creation of knowledge and discovery of mechanisms by which environmental exposures, singly and in combination, contribute to health risk across the life span, and translating findings into strategies to mitigate risks and improve human health. This mission is achieved by supporting scientific research and translating this research through community engagement and education. Thus, the EHSC provides the framework for the generation of novel research discoveries and their conversion into effective resources for scientists, public health officials, medical professionals, and communities.

PI_NAMEs: LAWRENCE, B PAIGE  (contact)

PROGRAM_OFFICER_NAME: THOMPSON, CLAUDIA L

PROJECT_TERMS: Address;Affect;Area;Binding;Biological Models;Biometry;Body System;career development;Chemical Exposure;Clinical;collaborative environment;Communities;community engagement;Community Health Education;community partners;community partnership;Complex;Core Facility;Creativeness;data to knowledge;Development;Disease;environmental agent;Environmental Exposure;Environmental Health;Environmental Impact;Environmental Risk Factor;Epidemiology;Epigenetic Process;Event;Faculty;Fostering;Foundations;Future;Generations;Goals;Health;health science research;Health Sciences;Human;human disease;improved;innovation;Institution;inter-institutional;International;International Aspects;Knowledge;Learning;Life;life span;Medical;member;Methods;Mission;Modernization;multidisciplinary;National Institute of Environmental Health Sciences;next generation;novel;Pilot Projects;Positioning Attribute;prevent;Prevention;Process;Program Development;programs;protective factors;Public Health;Research;Research Personnel;resilience;Resources;response;Risk;Risk Factors;risk mitigation;Science;Scientist;Shapes;skills;stem;stem cells;Structure;Students;success;supportive environment;synergism;Talents;tool;Training Programs;Translating;Translations

PROJECT_TITLE: Environmental Agents as Modulators of Disease Processes


NIH_SPENDING_CATS: Building Research Capacity at Institutions with Limited NIH Funding

ORG_CITY: OKLAHOMA CITY

ORG_NAME: UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR

ORG_STATE: OK

PI_NAMEs: AKINS, DARRIN R. (contact)

PROGRAM_OFFICER_NAME: ARORA, KRISHAN


NIH_SPENDING_CATS: Bioengineering;Nanotechnology

ORG_CITY: GREENSBORO

ORG_NAME: NORTH CAROLINA AGRI & TECH ST UNIV

PHR: This proposal seeks funding to acquire state-of-the-art THUNDER Imager Model Organism equipment that will be used to expand the scope of the funded NIH project in PI's laboratory.

PI_NAMEs: ZADEGAN, REZA  (contact)

PROGRAM_OFFICER_NAME: BERNAL, FEDERICO

PROJECT_TERMS: Animal Model;Circulation;Complex;Dedications;Disease;DNA;Endosomes;Environment;Equipment;Excretory function;Funding;Goals;imager;Imaging Device;In Vitro;in vivo;Laboratories;Metabolism;model organism;Molecular;nanorobot;receptor;Reporting;Research;Robot;Therapeutic;United States National Institutes of Health

PROJECT_TITLE: Developing DNA-Based Molecular Robots


NIH_SPENDING_CATS: Antimicrobial Resistance;Emerging Infectious Diseases;Infectious Diseases

ORG_CITY: BOSTON

ORG_NAME: HARVARD MEDICAL SCHOOL

ORG_STATE: MA

PHR: Project Narrative Bacterial infections remain a leading cause of death, a problem that is aggravated by the limited effectiveness of antibiotics for killing slow-growing and dormant bacteria, such as recurrent E. coli urinary tract infections. We propose to elucidate how bacteria can survive prolonged dormant metabolic states and how this affects pathogenesis and treatment. Identifying molecular mechanisms of adaptability and survival in slow growing bacteria may uncover new targets for novel antibiotics.

PI_NAMEs: BASAN, MARKUS THOMAS (contact)

PROGRAM_OFFICER_NAME: NGUYEN, HAN

PROJECT_TERMS: Affect;Antibiotic Resistance;antibiotic tolerance;Antibiotics;Bacteria;Bacterial Infections;Biology;Biophysics;Cause of Death;cell envelope;combat;Complex;cystic fibrosis patients;Data;Death Rate;Developed Countries;Effectiveness;Energy Metabolism;Escherichia coli;experience;Genes;Genetic;genetic resistance;Growth;insight;Laboratories;Membrane;Metabolic;Microbial Biofilms;Molecular;novel;Nutrient;Osmoregulation;Oxygen;Pathogenesis;pathogenic Escherichia coli;Pharmaceutical Preparations;Pharmacotherapy;Phenotype;Physiology;Positioning Attribute;Process;Proteomics;Pseudomonas aeruginosa infection;Recurrence;recurrent infection;Role;Tuberculosis;Urinary tract infection;Woman;wound healing

PROJECT_TITLE: Elucidating physiology of dormant bacteria to combat antibiotic persistence


ORG_CITY: LUBBOCK

ORG_NAME: TEXAS TECH UNIVERSITY

ORG_STATE: TX

PHR: Not Applicable. This proposal is for an NIGMS administrative supplement for equipment purchase.

PI_NAMEs: WANG, DEGENG  (contact)

PROJECT_TERMS: Administrative Supplement;Cells;equipment acquisition;National Institute of General Medical Sciences;Protein Isoforms

PROJECT_TITLE: Live Cell Isoform-specific Akt Analyses


ADMINISTERING_IC: EB

FUNDING_MECHANISM: OTHER RESEARCH-RELATED

IC_NAME: NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING

NIH_SPENDING_CATS: Back Pain;Bioengineering;Biomedical Imaging;Chronic Pain;Neurodegenerative;Neurosciences;Pain Research;Peripheral Neuropathy;Rehabilitation

ORG_NAME: MASSACHUSETTS GENERAL HOSPITAL

PHR: Project Narrative The project’s cornerstone is the integration of high-frequency neuromodulation, a method proven effective in invasive spinal cord stimulation therapies, into a non-invasive modality that targets the spinal structures non-invasively. By creating a compact, multilayer High-Frequency Trans-Spinal Magnetic Stimulation (HF-TSMS) system, we aim to modulate the neural pathways associated with pain transmission and management directly, offering a significant advantage over traditional surgical intervention methods. HF-TSMS seeks to mitigate the pain associated with sciatica, potentially reducing or eliminating the reliance on pharmacological treatments, which are often accompanied by significant side effects and the risk of addiction.

PI_NAMEs: BONMASSAR, GIORGIO  (contact)

PROGRAM_OFFICER_NAME: BITTMANN, MORIA FISHER

PROJECT_TERMS: addiction;Address;Advanced Development;Anatomy;Animal Model;animal pain;Animal Testing;Animals;Area;Arizona;Back;Back Pain;Behavior;biomaterial compatibility;Central Nervous System;Cephalic;Chest;chronic neuropathic pain;chronic pain;clinical application;clinical trial readiness;Clinical Trials;design;Devices;diagnostic tool;Dorsal;dorsal column;Effectiveness;Eligibility Determination;Ensure;Equipment Malfunction;Evaluation;Excision;experience;experimental study;Family suidae;Focused Ultrasound;Focused Ultrasound Therapy;Frequencies;Functional Magnetic Resonance Imaging;Gel;General Hospitals;Heating;Human;human model;Implant;implantation;Implantation procedure;Infection;innovation;Lead;Leg;magnetic field;Magnetic Resonance Imaging;Magnetism;manufacture;Massachusetts;medical attention;meetings;Methods;migration;miniaturize;Modality;Muscle;Nerve;nerve injury;Neural Pathways;neural stimulation;Neurologic;Neuropathy;neuroregulation;Neurostimulation procedures of spinal cord tissue;Neurosurgeon;novel;Operative Surgical Procedures;opioid use;Outcome;Pain;Pain in lower limb;Pain management;Pain Measurement;pain model;pain perception;pain reduction;pain relief;painful neuropathy;Paresthesia;Pathway interactions;Patients;Performance;Peripheral;peroneal nerve;Pharmaceutical Preparations;Pharmacological Treatment;Physiologic pulse;Pilot Projects;pilot trial;porcine model;Positioning Attribute;pre-clinical;preclinical study;Procedures;Rattus;Research;Resistance;response;Risk;Rodent;Rodent Model;Safety;Sciatica;Secure;side effect;simulation;Spinal;Spinal Cord;Spinal Ganglia;Structure;Structure of radial nerve;success;System;Techniques;Technology;Testing;Therapeutic;Thoracic spinal cord structure;Time;time use;tool;transmission process;Universities;Validation;Work

PROJECT_TITLE: Transspinal Magnetic Stimulation for the Treatment of Neuropathic Pain

STUDY_SECTION_NAME: Special Emphasis Panel[ZRG1 NV-Q (91)]


NIH_SPENDING_CATS: Microbiome;Nutrition

ORG_CITY: RALEIGH

ORG_NAME: NORTH CAROLINA STATE UNIVERSITY RALEIGH

PHR: Project Narrative The proposed research is relevant to public health because understanding how interactions between dietary protein and the intestinal microbiota impact human health will allow rational design of customized therapeutic interventions that consider detrimental and beneficial impacts of different dietary protein sources. Thus, the proposed research is relevant to the part of the NIH's mission that pertains to developing fundamental knowledge that will reduce the burdens of human illness. The research is also relevant to the mission of the NIGMS as it will provide new, broadly applicable methods to increase the understanding of functional interactions between humans and their microbiota, which lays the foundation for advances in disease diagnosis, treatment and prevention.

PI_NAMEs: KLEINER, MANUEL  (contact)

PROGRAM_OFFICER_NAME: KEANE-MYERS, ANDREA

PROJECT_TERMS: Biochemical;Biological Assay;cellular imaging;Communities;Consumption;Development;Diet;Dietary Proteins;Disease;disease diagnosis;Environment;Foundations;Gene Expression;Goals;gut microbiota;Health;host microbiota;host-associated microbial communities;Human;Human Microbiome;human microbiota;In Vitro;insight;Intestines;Knowledge;Laboratory Study;Mass Spectrum Analysis;Measurement;member;metabolic abnormality assessment;metabolomics;Metagenomics;metaproteomics;Methods;microbial;microbial community;microbiota;Mission;National Institute of General Medical Sciences;novel strategies;Physiology;Prevention;Proteins;Public Health;rational design;Reproducibility;Research;Resolution;response;Site;Source;Symbiosis;therapeutic development;Therapeutic Intervention;tool;United States National Institutes of Health

PROJECT_TITLE: Metaproteomics to investigate intestinal microbiota-host and -diet interactions


FUNDING_MECHANISM: TRAINING, INSTITUTIONAL

ORG_CITY: Newark

ORG_NAME: UNIVERSITY OF DELAWARE

ORG_STATE: DE

PHR: PROJECT NARRATIVE Biomedical research is rapidly shifting towards data-driven discovery powered by Artificial Intelligence (AI)/Machine Learning (ML), but many researchers lack the skills to unlock its potential. This project proposes a cloud-based NIGMS Sandbox module, "Practical Data-Centric AI/ML for Biomedical Researchers," to bridge this gap. The module dives into essential topics like data preparation, model building and interpretation, empowering biomedical researchers with hands-on skills and real-world tools through accessible Google Cloud platform, capstone projects, and diverse learning formats; therefore, this accessible learning journey will equip researchers to ethically apply AI/ML to their research, contribute to a future of AI-driven advancements, and elevate research quality through responsible practices.

PI_NAMEs: POLSON, SHAWN W (contact);SINGH, ABHYUDAI ;WU, CATHY H.

PROGRAM_OFFICER_NAME: DAVANI, BEHROUS

PROJECT_TERMS: Data;Research Personnel

STUDY_SECTION: TWD

STUDY_SECTION_NAME: Training and Workforce Development Study Section - A[TWD-A]


NIH_SPENDING_CATS: Clinical Research;Health Disparities Research;Health Disparities and Racial or Ethnic Minority Health Research;Prevention;Rural Health;Social Determinants of Health

ORG_CITY: LOUISVILLE

ORG_DEPT: PHARMACOLOGY

ORG_NAME: UNIVERSITY OF LOUISVILLE

ORG_STATE: KY

PHR: The goal of the University of Louisville Center for Integrative Environmental Health Sciences is to understand how exposure to environmental toxicants interacts with life style factors and how these co-exposures impact human health and disease, as modified by life stage and gender. This knowledge will provide better prevention and intervention strategies to improve the health of the people in western Kentucky.

PI_NAMEs: STATES, J CHRISTOPHER (contact)

PROJECT_TERMS: addiction;Adult;Agriculture;Alcohol consumption;Alcohols;Area;Basic Science;Biological Models;Biometry;built environment;Cardiovascular system;career development;Chemicals;Childhood;Chronic;Chronic Disease;citizen science;Clinical;Clinical Trials;Clinical Trials Unit;Collaborations;Communication;Communities;community based participatory research;community based research;community collaboration;community engagement;community partners;Complex;Core Facility;County;design;Development;Diabetes Mellitus;Diet;Disease;Disease Outcome;Ecosystem;Education;Environment;Environmental Health;Environmental Risk Factor;Etiology;Exposure to;Funding;Gender;Genetic;Genetic Predisposition to Disease;Genotype;Geographic Locations;Goals;Grant;Health;Health Personnel;health science research;Health Sciences;Human;improved;Individual;Industrialization;Informatics;Interdisciplinary Study;Interest Group;Intervention;Investigation;Kentucky;Knowledge;Laboratories;Leadership;Life;Life Style;lifestyle factors;Malignant Neoplasms;man;Measurement;member;Mental disorders;Mentors;Mentorship;Metals;metropolitan;Modification;multidisciplinary;next generation;Obesity;Organ;Pathogenesis;Persons;Pilot Projects;Play;pollutant;Pollution;Polychlorinated Biphenyls;Positioning Attribute;Predisposition;Prevention strategy;Program Development;programs;recruit;Research;Research Personnel;Resources;response;Role;Rural;rural environment;rural healthcare;skills;socioeconomics;Strategic vision;stressor;tobacco exposure;tobacco products;Toxic Environmental Substances;Toxicant exposure;toxicant interaction;Toxicology;Training;Translating;Translations;United States;Universities;urban setting;Vinyl Chloride;Youth

STUDY_SECTION: EHS

STUDY_SECTION_NAME: Environmental Health Sciences Review Committee[EHS(P)]


NIH_SPENDING_CATS: Neurosciences

ORG_CITY: CHARLOTTESVILLE

ORG_NAME: UNIVERSITY OF VIRGINIA

ORG_STATE: VA

PHR: Project Narrative Cell migration is an important process that occurs in many physiological and pathophysiological contexts, including embryogenesis, inflammation, wound healing and tumor metastasis. Our published and preliminary data indicate a previously unrecognized role for contacts between the endoplasmic reticulum and the plasma membrane in the migration of metastatic cells. This project seeks to understand the mechanisms by which calcium influx and lipid transfer are coordinated at these sites, and how they regulate cell motility.

PI_NAMEs: CASANOVA, JAMES E. (contact)

PROGRAM_OFFICER_NAME: AINSZTEIN, ALEXANDRA M

PROJECT_TERMS: Actins;Adhesions;ADP-Ribosylation Factors;AMPA Receptors;Attention;Automobile Driving;Calcium;Calcium Channel;Calcium Signaling;Cell membrane;cell motility;Cell Physiology;Cells;Complex;Cytoskeleton;Data;Embryonic Development;Endocytosis;Endoplasmic Reticulum;excitatory neuron;Failure;Family;Focal Adhesions;Goals;Guanosine Triphosphate Phosphohydrolases;Human;Inflammation;Integrins;Lecithin;lipid transfer protein;Lipids;member;Membrane;Microtubules;migration;Molecular;Mutation;Neoplasm Metastasis;Neuroglia;novel;Patients;Phosphatidylinositols;Physiological;postsynaptic;Process;protein function;Protein Isoforms;Proteins;Publishing;Reporting;response;rho;Role;Site;trafficking;tumor;Vesicle;wound healing;X-linked intellectual disability

PROJECT_TITLE: ARFs, ORPs and the control of cell migration


NIH_SPENDING_CATS: Complementary and Integrative Health;Natural Products

ORG_CITY: LA JOLLA

ORG_DEPT: ZOOLOGY

ORG_NAME: UNIVERSITY OF CALIFORNIA, SAN DIEGO

ORG_STATE: CA

PHR: Public Health Relevance Statement Natural product compounds occupy a central role in medicine in providing lifesaving drugs. Supply challenges however often constrain the biomedical potential of promising bioactive natural products from rare or environmentally sensitive marine organisms. Our discovery of the first terpenoid biosynthesis genes in marine animals and amongst the first in marine algae towards flagship bioactive molecules like the anti-inflammatory pseudodopterosin and anticancer halomon offers a path to bioengineering drug leads from marine organisms from sustainable laboratory microbial fermentation.

PI_NAMEs: CHANG, GEOFFREY A;MOORE, BRADLEY S (contact)

PROGRAM_OFFICER_NAME: BOND, MICHELLE RUEFFER

PROJECT_TERMS: Algae;Anabolism;Animals;anti-cancer;Anti-Inflammatory Agents;bioactive natural products;Biological;Biomedical Engineering;Cells;Chemicals;Clinical;coral;Environment;Enzymes;Equipment;Eukaryota;Fermentation;Genes;Genetic Engineering;Genome;halogenation;Knowledge;Laboratories;Life;Logic;marine;Marine Invertebrates;marine organism;Medicine;microbial;Mining;Molecular;multidisciplinary;Natural Products;novel therapeutics;Outcomes Research;Pathway interactions;Pharmaceutical Preparations;Plants;preclinical evaluation;Production;Property;pseudopterosins;public health relevance;Research Project Grants;Role;terpene synthase;Terpenes;Terpenoid Biosynthesis Pathway

PROJECT_TITLE: Equipment Supplement - Biosynthesis of marine terpenoid natural products

STUDY_SECTION: CSB

STUDY_SECTION_NAME: Chemical Synthesis and Biosynthesis Study Section[CSB]


PHR: Project Narrative Defects in the internal organization of cells and the transport mechanisms of their proteins and membranes underlie the pathogenesis of many human diseases including cancer, neurodegeneration (e.g., Alzheimer’s, Parkinson’s, ALS), brain injury and metabolic disorders. This research program aims to advance our knowledge of how transport within cells is spatially controlled by a unique family of proteins called septins. The proposed research will advance basic knowledge of cell biology, which in the long-term will be used to design better treatments that can reverse or slow down disease and/or to regenerate ailing cells and tissues.

PI_NAMEs: SPILIOTIS, ELIAS T (contact)

PROJECT_TERMS: 3-Dimensional;Alzheimer's Disease;Apical;Brain Injuries;Cell Polarity;cell type;Cells;Cellular biology;Defect;design;Destinations;Disease;Docking;dynactin;Dynein ATPase;Epithelium;Family;GTP-Binding Proteins;Guanosine Triphosphate Phosphohydrolases;Homeostasis;human disease;In Vitro;innovation;insight;Kinesin;Knowledge;Laboratories;Lysosomes;Maintenance;Malignant Neoplasms;Membrane;Membrane Protein Traffic;Metabolic;Metabolic Diseases;Microtubules;Morphogenesis;Motility;Motor;Natural regeneration;Nerve Degeneration;Neurons;neurotransmission;novel;Organoids;Outcome;Parkinson Disease;Pathogenesis;Phosphorylation;Phosphotransferases;Physiological;polarized cell;Positioning Attribute;programs;Protein Family;Protein Sortings;Proteins;reconstitution;recruit;regenerative therapy;Regulation;Research;response;Role;Route;scaffold;Signal Transduction;single molecule;Space Perception;Specificity;Structure;superresolution microscopy;Testing;Tissues;tool;trafficking;Transmembrane Transport;Vesicle

PROJECT_TITLE: Spatial control of membrane traffic by septin GTPases


NIH_SPENDING_CATS: Genetics

ORG_CITY: MILWAUKEE

ORG_NAME: MEDICAL COLLEGE OF WISCONSIN

ORG_STATE: WI

PHR: PROJECT NARRATIVE The abscission checkpoint is a cell cycle checkpoint that arrests cytokinetic abscission to ensure the upstream events of mitosis are completed properly before cells finally divide. This proposal will integrate new molecular signaling pathways that contribute to the maintenance of the abscission checkpoint as well as characterize cellular programs that promote faithful abscission during an abscission arrest. In the long term, our research may enable new therapeutic strategies, so that we are better able to diagnose and treat diseases when this checkpoint fails.

PI_NAMEs: WENZEL, DAWN  (contact)

PROGRAM_OFFICER_NAME: MILLER, RITA K

PROJECT_TERMS: Address;Administrative Supplement;Autophagocytosis;Biological Assay;cancer cell;Cell Cycle Checkpoint;Cell Proliferation;Cells;Chromosomal Breaks;Chromosome Segregation;Coupled;daughter cell;Development;Diagnosis;Disease;DNA;DNA biosynthesis;DNA Damage;Ensure;Event;Excision;Funding;glucose metabolism;Goals;innovation;insight;interdisciplinary approach;Link;Maintenance;Malignant Neoplasms;Measures;Metabolic;micronucleus;Mitosis;Mitotic;Molecular;novel;novel therapeutic intervention;Nuclear Pore;Pathway interactions;physical separation;Post-Translational Protein Processing;premature;programs;Proteins;recruit;Regulation;Research;Signal Pathway;Signal Transduction;small molecule;Structure;Therapeutic;Thermodynamics;Work

PROJECT_TITLE: Ins and Outs of Abscission Checkpoint Signaling: Molecular Mechanisms Safeguarding Abscission


ORG_CITY: WEST LAFAYETTE

ORG_DEPT: ENGINEERING (ALL TYPES)

ORG_NAME: PURDUE UNIVERSITY

ORG_STATE: IN

PHR: Public Health Relevance/Narrative Fluorescence microscopy is an increasingly critical tool in biomedical research. The proposed research seeks to advance public health by allowing direct visualization at the nanoscale of subcellular mechanisms and fostering new insights into a range of biological processes. Reflecting NJH's core mission, these efforts will support the development of fundamental knowledge that enhances human health.

PI_NAMEs: HUANG, FANG  (contact)

PROGRAM_OFFICER_NAME: YEH, ALVIN TIEN-WEI

PROJECT_TERMS: 3-Dimensional;Actins;analytical method;Biological Process;biological research;Biomedical Research;Budgets;cell fixing;Cell Lineage;Cell Physiology;Cells;cellular imaging;Cellular Structures;Chromatin;constriction;Deterioration;Development;diffraction of light;Dyes;Dynein ATPase;Epigenetic Process;Event;Fission Yeast;Fluorescence;Fluorescence Microscopy;Fostering;Gene Expression Profile;Goals;Growth Cones;Health;Human;Image;imaging system;Imaging technology;improved;innovation;insight;instrument;Knowledge;Label;macromolecular assembly;Maps;Methods;Microfilaments;Microscopy;Microtubules;Mission;Modernization;Molecular;molecular imaging;Molecular Motors;Myosin ATPase;nanoscale;neuronal growth;novel;optical imaging;Phase;Photons;Positioning Attribute;Proteins;Public Health;public health relevance;reconstruction;Research;Resolution;Sampling;single molecule;Specificity;Specimen;structural imaging;Structural Models;Structure;superresolution imaging;superresolution microscopy;Surface;Thinness;Three-Dimensional Imaging;Time;Tissues;tool;ultra high resolution;Visualization

PROJECT_TITLE: Ultra-high resolution structural and molecular imaging of cells and tissues


NIH_SPENDING_CATS: Clinical Research;Emerging Infectious Diseases;Health Disparities and Racial or Ethnic Minority Health Research;Infectious Diseases;Racial and Ethnic Minority Health Research

ORG_CITY: SAN JUAN

ORG_NAME: UNIVERSITY OF PUERTO RICO MED SCIENCES

ORG_STATE: PR

PHR: NARRATIVE Through this supplement, the requested equipment will greatly enhance the biorepositories capacity of the Hispanic Alliance of Clinical and Translational Research (Alliance) to support clinical and translational research in diseases highly prevalent in the Hispanic population and Puerto Rico. Specifically, this supplement will increase the capacity of the Alliance Biorepository Network (ABN) for biological samples storage and processing, single-cell “omics” analyses, and access to basic equipment and facilities for investigators in Puerto Rico.

PI_NAMEs: LUCIANO, CARLOS A. (contact);MOROU-BERMUDEZ, EVANGELIA

PROGRAM_OFFICER_NAME: BISSONETTE, GREG

PROJECT_TERMS: biobank;Biological;Biological Markers;Biological Specimen Banks;Cardiometabolic Disease;Cells;Clinical Investigator;Clinical Research;Data;Data Collection;Disease;Emerging Communicable Diseases;Equipment;Funding;Health;Health Sciences;Hispanic;Hispanic Populations;improved;in vitro Model;Infrastructure;Institution;Malignant Neoplasms;Measures;Molecular;parent project;Participant;Patients;Population;Process;Puerto Rico;recruit;Research;Research Infrastructure;Research Personnel;research study;Research Support;Resources;Sampling;single cell analysis;Specimen;Systems Analysis;Technology;Translational Research;translational scientist;Universities

PROJECT_TITLE: Hispanic Alliance for Clinical and Translational Research (Alliance)


ORG_CITY: CHICAGO

ORG_NAME: UNIVERSITY OF CHICAGO

PHR: Project Narrative Methods capable of defining protein complex dynamic directly in living systems hold tremendous promise for defining the molecular mechanisms that underlie normal and disease signaling. Here we propose to develop and validate several complementary light-dependent proximity profiling platforms capable of detecting protein interaction dynamics in live cells with high spatial and temporal resolution, as well as minimal perturbation to the cellular environment. Application of these platforms to map the protein social network remodeling in response dysregulated cellular metabolism and redox regulation will provide an unparalleled view of disease signaling, as well as serve as a blueprint for future studies of other dynamic signaling pathways with photoproximity platforms.

PI_NAMEs: MOELLERING, RAYMOND E (contact)

PROGRAM_OFFICER_NAME: SALVADOR MORALES, CAROLINA

PROJECT_TITLE: Equipment Supplement: Spatial Photoproximity Profiling of Dynamic Protein Complexes


ADMINISTERING_IC: HL

IC_NAME: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE

NIH_SPENDING_CATS: Congenital Structural Anomalies;Eye Disease and Disorders of Vision;Lung;Pediatric;Primary Immunodeficiencies;Pulmonary Fibrosis;Rare Diseases

ORG_CITY: PROVIDENCE

ORG_DEPT: MICROBIOLOGY/IMMUN/VIROLOGY

ORG_NAME: BROWN UNIVERSITY

ORG_STATE: RI

PHR: Project Narrative Hermansky-Pudlak Syndrome (HPS) is a group of inherited autosomal recessive disorders caused by genetic mutations that alter the trafficking of lysosomal-related organelles/vesicles. In a subset of patients with HPS-1 and HPS-4, pulmonary fibrosis develops in the fourth or fifth decade of life, and is a major cause of morbidity and mortality. Experiments proposed in this project will define the cellular and molecular mechanisms of CHI3L1 receptor system, and whether CHI3L1 and its receptor systems are plausible targets to treat HPS-associated lung disease.

PI_NAMEs: ZHOU, YANG  (contact)

PROGRAM_OFFICER_NAME: VUGA, LOUIS J

PROJECT_TERMS: Apoptosis;Attenuated;autosome;Biogenesis;Biology;Blood Platelets;Cell Death;cell injury;cell motility;cell type;Cells;CHI3L1 gene;Chitinase;comparison control;Complex;Cytoplasmic Granules;Defect;Deposition;Development;Disease;Disease susceptibility;DNA Sequence Alteration;Ear;effective therapy;Epithelial Cells;experimental study;Fibroblasts;Fibrosis;fMet-Leu-Phe receptor;Galectin 3;Genes;Hemorrhage;Hermanski-Pudlak Syndrome;Human;idiopathic pulmonary fibrosis;Impairment;In Vitro;in vivo;indium-bleomycin;Individual;Inherited;injury and repair;Investigation;Laboratories;Life;Lung;Lung Diseases;Lymphoid Cell;Mediating;Melanosomes;Modeling;Molecular;Morbidity - disease rate;mortality;mouse model;Mus;Mutation;Myofibroblast;neutralizing antibody;Oculocutaneous Albinism;Organelles;Pathway interactions;patient subsets;Patients;Phenotype;Pigmentation physiologic function;Predisposition;Process;Production;Proliferating;protective effect;Proteins;prototype;Pulmonary Fibrosis;receptor;Regulation;repaired;response;Role;Severity of illness;Signal Transduction;System;Testing;Th2 Cells;Therapeutic;Therapeutic Effect;therapeutic target;trafficking;Vesicle

PROJECT_TITLE: Targeting CHI3L1 and its receptors in Hermansky-Pudlak Syndrome-associated lung disease

STUDY_SECTION: LIRR

STUDY_SECTION_NAME: Lung Injury, Repair, and Remodeling Study Section[LIRR]


ED_INST_TYPE: SCHOOLS OF PHARMACY

NIH_SPENDING_CATS: Bioengineering;Genetics

ORG_CITY: SAN FRANCISCO

ORG_NAME: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO

PHR: PROJECT NARRATIVE During cell division, each daughter cell must inherit exactly one copy of each chromosome as errors can lead to cancer and birth defects. Two large cellular machines are responsible for segregating chromosomes: the spindle and kinetochore. Here we probe how these machines’ building blocks together give rise to their function, aiming to uncover the mechanical design principles driving chromosome segregation and to ultimately better target dividing cells for therapeutic purposes.

PI_NAMEs: DUMONT, SOPHIE  (contact)

PROJECT_TERMS: Acute;Administrative Supplement;Anaphase;Architecture;Automobile Driving;Binding;biophysical techniques;Cell division;Cells;Chromosome Segregation;Chromosomes;Congenital Abnormality;daughter cell;Decision Making;design;Equipment;Event;Goals;grasp;In Vitro;in vivo;Inherited;innovation;Kinetochores;Knowledge;Malignant Neoplasms;Mammalian Cell;Mammalian Chromosomes;Mechanics;meter;Microscopy;Microtubules;Mitosis;Modeling;Molecular;molecular scale;Needles;optogenetics;Parents;physical process;physical property;programs;reconstitution;Research Support;Role;Shapes;Signal Transduction;Stress;Structure;System;Testing;Therapeutic;Time;tool;Work

PROJECT_TITLE: Microscopy system upgrade


NIH_SPENDING_CATS: Bioengineering

ORG_CITY: TALLAHASSEE

ORG_NAME: FLORIDA STATE UNIVERSITY

ORG_STATE: FL

PHR: NMR spectroscopy uses strong magnets and radio frequency technology in the study of metabolomics and the structural biology of proteins, membrane proteins and complexes they form, providing powerful means for understanding how structure, dynamics, and chemistry drive functional mechanisms. We propose to purchase new equipment essential to improving the world’s strongest magnet used for NMR spectroscopy. The improved facility will be available for biologists, biomedical engineers and all scientists with projects that require its unique capabilities.

PI_NAMEs: BREY, WILLIAM W;LONG, JOANNA R;SCHURKO, ROBERT  (contact)

PROGRAM_OFFICER_NAME: BARNES, CHARLES ASHLEY

PROJECT_TERMS: biological systems;Biomedical Engineering;Chemicals;Chemistry;Complex;cyber security;data exchange;Development;Electronics;Environment;Equipment;experimental study;High temperature of physical object;Hybrids;improved;instrument;Measurement;Membrane Proteins;metabolomics;NMR Spectroscopy;parent project;parenting intervention;Proteins;radio frequency;Resources;Running;Scientist;Series;Speed;structural biology;Structure;System;Technology;Textiles

PROJECT_TITLE: National Resource for Advanced NMR Technology


NIH_SPENDING_CATS: Genetics;Human Genome

PHR: Project Narrative Epigenetic information carriers and their signaling consequences play critical roles in all aspects of eukaryotic genome management, but many of their mechanisms remain poorly understood. My research program spans a host of traditional disciplines (discovery biochemistry, technology development, quantitative genomics, chemical biology, biophysics, and cell biology) with the goal of developing fundamental mechanistic understanding of epigenetic information systems.

PI_NAMEs: RUTHENBURG, ALEXANDER JACKSON (contact)

PROGRAM_OFFICER_NAME: CHOW, CHI-WING

PROJECT_TERMS: Area;Binding;Biochemical;Biochemistry;Biology;Biophysics;Cell Nucleus;Cellular biology;Chemicals;Chromatin;Chromatin Structure;Cognition;Complex;Development;Discipline;Disease;DNA;DNA Modification Process;DNA Sequence;environmental change;Epigenetic Process;Family;Funding;Future;Genetic Transcription;Genome;Genomics;Goals;Hepatocyte;histone modification;Histones;Information Systems;Length;Modification;Molecular;National Institute of General Medical Sciences;Neurons;Nuclear;Nucleosomes;Physiological;Play;programs;Proteins;Research;RNA;Role;Signal Pathway;Signal Transduction;success;technology development;tool;tool development;Transcription Coactivator;transcription factor;Transcriptional Activation;Untranslated RNA;Variant;Work

PROJECT_TITLE: Defining the mechanisms of epigenetic information flow