ORG_DEPT: UROLOGY

PHR: PROJECT NARRATIVE (RELEVANCE) The molecular chaperone Hsp90 is essential for maintaining protein homeostasis, which is critical in many maladies. This project will provide information on complex interplay between regulatory components of the Hsp90 chaperone in cells, which can also be uti- lized to enhance the sensitivity of cells to Hsp90 inhibitors.

PI_NAMEs: MOLLAPOUR, MEHDI  (contact)

PROGRAM_OFFICER_NAME: PHILLIPS, ANDRE W

PROJECT_TERMS: ATP phosphohydrolase;Autoimmune Diseases;Binding;Biochemical;Biological Assay;Biophysics;Cells;chaperone machinery;Client;Clinical Trials;Complex;Coupled;Deceleration;Disease;drug sensitivity;enhancing factor;Goals;Heat-Shock Proteins 90;HSP 90 inhibition;Inflammatory;inhibitor;Knowledge;Malignant Neoplasms;Molecular;Molecular Chaperones;Neurodegenerative Disorders;Pharmaceutical Preparations;Phosphorylation;Phosphotransferases;Post-Translational Protein Processing;preclinical study;Proteins;proteostasis;Regulation;Signal Transduction;Signal Transduction Pathway;targeted treatment;TSC1 gene;Ubiquitination;Vision;Work

PROJECT_TITLE: Regulatory mechanisms of the Hsp90 chaperone machinery.


ADMINISTERING_IC: GM

ARRA_FUNDED: N

ED_INST_TYPE: SCHOOLS OF MEDICINE

FUNDING_MECHANISM: NON-SBIR/STTR RPGS

IC_NAME: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

NIH_SPENDING_CATS: Genetics

ORG_CITY: CHICAGO

ORG_COUNTRY: UNITED STATES

ORG_DEPT: BIOCHEMISTRY

ORG_FIPS: US

ORG_NAME: NORTHWESTERN UNIVERSITY AT CHICAGO

ORG_STATE: IL

PHR: Project Narrative    Chromatin  is  a  dynamic  structure  that  requires  constant  assembly  and  disassembly.  Histone  proteins  are  the  subunits of chromatin and require dedicated chaperone to orchestrate their function. This application will define  a  novel  histone  chaperone  pathway  for  new  and  evicted  histone  ad  define  a  new  mode  of  crosstalk  between  chromatin assembly and nucleotide synthesis.

PI_NAMEs: FOLTZ, DANIEL RICHARD (contact)

PROGRAM_OFFICER_NAME: CHOW, CHI-WING

PROJECT_TERMS: Acetylation;Binding;Chromatin;Chromatin Modeling;Dedications;Deposition;DNA;DNA biosynthesis;Elements;Enzymes;Genetic Transcription;Genome;HAT1 gene;histone acetyltransferase;Histone H3;Histone H4;Histones;Mentored Clinical Scientist Development Program;Metabolic Control;Modification;Molecular Chaperones;novel;Nuclear;Nucleosomes;nucleotide metabolism;Pathway interactions;Post-Translational Protein Processing;prevent;Process;Protein Subunits;Proteins;Slide;Structure;Time;Variant;Work

PROJECT_TITLE: Histone chaperone networks for new and evicted histones

STUDY_SECTION: MGB

STUDY_SECTION_NAME: Molecular Genetics B Study Section[MGB]


NIH_SPENDING_CATS: Infectious Diseases;Prevention

ORG_DEPT: MICROBIOLOGY/IMMUN/VIROLOGY

PHR: PROJECT NARRATIVE (from the parent award)  The formation of the 100S ribosome is a universal hallmark of both beneficial and pathogenic bacteria and is important for bacterial survival. Completion of this study may offer insights for developing novel treatments and preventive interventions for infections and for the improvement of probiotics.

PI_NAMEs: YAP, M.-N. FRANCES (contact)

PROGRAM_OFFICER_NAME: REDDY, MICHAEL K

PROJECT_TERMS: Bacteria;bacterial genetics;Biochemistry;Biogenesis;Biological;combat;Cytoplasmic Protein;Eukaryota;Goals;Gram-Negative Bacteria;Hibernation;improved;Infection;innovation;insight;interdisciplinary approach;life span;Microscopy;Molecular;mutant;novel;parent project;pathogenic bacteria;Pathogenicity;Phosphodiesterase I;prevent;preventive intervention;Probiotics;rapid growth;Regulation;Resolution;ribonuclease R;Ribonucleases;Ribosomes;Role;segregation;spatiotemporal;Staphylococcus aureus;structural biology;Translating;Translation Initiation;Translations;unpublished works;Virulence Factors

PROJECT_TITLE: Regulation and function of bacterial 100S ribosome - Admin Equip Supplement on R01GM121359-07

STUDY_SECTION: PCMB

STUDY_SECTION_NAME: Prokaryotic Cell and Molecular Biology Study Section[PCMB]


ADMINISTERING_IC: NS

IC_NAME: NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE

NIH_SPENDING_CATS: Autoimmune Disease;Bioengineering;Biomedical Imaging;Brain Disorders;Multiple Sclerosis;Neurodegenerative;Neurosciences

ORG_CITY: DALLAS

ORG_DEPT: RADIATION-DIAGNOSTIC/ONCOLOGY

ORG_NAME: UT SOUTHWESTERN MEDICAL CENTER

ORG_STATE: TX

PHR: Project Narrative The goal of this project is to develop novel high-resolution magnetic resonance imaging techniques to detect and monitor the demyelination and axon damage processes in both white matter and cerebral cortex, especially in the early stage of multiple sclerosis. The successful outcome of the proposed project will advance and benefit the understanding the complex mechanism of multiple sclerosis and early diagnosis of the disease. Page 1 of 1

PI_NAMEs: WANG, NIAN  (contact)

PROGRAM_OFFICER_NAME: UTZ, URSULA

PROJECT_TERMS: 3-Dimensional;Acute;Affect;Anatomy;Animal Model;anterior commissure;Atlases;Autoimmune;autoimmune inflammation;Axon;axon injury;biophysical model;Brain;Brain imaging;brain magnetic resonance imaging;Central Nervous System Diseases;Cerebral cortex;Characteristics;chronic demyelination;Clinical;clinical application;Complex;connectome;contrast imaging;Corpus Callosum;Coupled;Cuprizone;Databases;Demyelinations;Dendrites;density;Development;Diagnosis;Diffusion;Diffusion Magnetic Resonance Imaging;Dimensions;Disease;disease diagnosis;Disease Progression;early detection biomarkers;Early Diagnosis;Early treatment;Exhibits;External Capsule;Functional disorder;Goals;gray matter;Histology;Human;Image;image archival system;imaging biomarker;imaging modality;Imaging Techniques;Immunohistochemistry;improved;in vivo;indexing;Inflammation Process;insight;interest;Investigation;Iron;Knowledge;Lesion;Light;Magnetic Resonance Imaging;Magnetism;Maps;Measures;meter;Methods;Microscopic;microscopic imaging;Microscopy;Modeling;Monitor;mouse model;Multiple Sclerosis;Multiple Sclerosis Lesions;Mus;Myelin;Nerve Degeneration;Nervous System Disorder;Neurites;Neurodegenerative Disorders;Neurons;Non-Invasive Detection;novel;Oligodendroglia;Outcome;Pathologic;Pathology;Persons;Predisposition;Process;prognostic;Property;Protocols documentation;quantitative imaging;reconstruction;Relaxation;remyelination;Resolution;Rodent;Role;Sampling;Site;Specificity;Structure;Symptoms;Techniques;Technology;Testing;Time;Tissues;tractography;Transgenic Mice;water diffusion;white matter

PROJECT_TITLE: Characterization of Whole Brain Demyelination and Axon Damage Using High-resolution Magnetic Resonance Imaging

STUDY_SECTION: EITN

STUDY_SECTION_NAME: Emerging Imaging Technologies in Neuroscience Study Section[EITN]


ED_INST_TYPE: SCHOOLS OF ARTS AND SCIENCES

ORG_CITY: EUGENE

ORG_DEPT: BIOLOGY

ORG_NAME: UNIVERSITY OF OREGON

ORG_STATE: OR

PHR: PROJECT NARRATIVE Accurate chromosome segregation and repair of DNA breaks during sperm and egg development are essential for preventing infertility, miscarriages, birth defects, and cancer. Developing sperm and eggs accomplish this through specific exchanges of DNA, called recombination events. The proposed research will reveal the mechanisms of these recombination events to increase understanding of fundamental processes critical to human health.

PI_NAMEs: LIBUDA, DIANA ELIZABETH (contact)

PROGRAM_OFFICER_NAME: MACARTHUR, LINDA

PROJECT_TERMS: Biological Assay;Biological Models;Caenorhabditis elegans;Cell division;Cell Physiology;Chromosome Segregation;Chromosome Structures;Chromosomes;Congenital Abnormality;Copy Number Polymorphism;Detection;Development;digital;DNA;DNA Double Strand Break;DNA Repair;egg;Ensure;Equipment;Event;experimental study;Genetic Recombination;Genetic Variation;Genome;genome integrity;genomic data;Germ Cells;Goals;Haploidy;Health;Human;in vivo;Infertility;instrument;Malignant Neoplasms;Meiosis;Meiotic Prophase I;Meiotic Recombination;Molecular Profiling;Mutation;Outcome;Pathway interactions;Population;prevent;Process;programs;Proteins;Publishing;repaired;Research;Single Nucleotide Polymorphism;Sister Chromatid;sperm cell;Spontaneous abortion;System;Validation;Work

PROJECT_TITLE: Recombination pathway and partner choices during meiosis


ED_INST_TYPE: HOSPITALS

NIH_SPENDING_CATS: Biotechnology;Genetics;Human Genome

ORG_CITY: HOUSTON

ORG_DEPT: BIOSTATISTICS & OTHER MATH SCI

ORG_NAME: UNIVERSITY OF TX MD ANDERSON CAN CTR

PHR: NOT-GM-24-021 – NARRATIVE – HART  Highly multiplexed genetic perturbations offer huge potential beyond fitness screens. High throughput microscopy offers a tractable method for combining complex genetic perturbation with multiparameter functional assays. Here we propose the purchase of a Nikon Eclipse Ji microscopy system to facilitate complex data collection to decipher the complexity and redundancy of mammalian signaling pathways. Using our next- generation CRISPR/Cas12a polygenic knockout system, we combine custom CRISPR arrays targeting signaling effectors and cell surface “barcodes”, then use immunofluorescence readouts of cell state across dozens of targets – two to four per well in a 96 well plate -- to understand the dynamics of cell signaling.

PI_NAMEs: HART, GLEN TRAVER (contact)

PROGRAM_OFFICER_NAME: MASKERI, BAISHALI

PROJECT_TERMS: Antibodies;antibody detection;Bar Codes;Bayesian Network;Biological;Biological Assay;causal model;cell behavior;Cell Line;Cell model;Cell surface;cell type;Cells;Cellular biology;cellular imaging;Cellular Morphology;Clustered Regularly Interspaced Short Palindromic Repeats;Code;combinatorial;Complex;complex data;Core Facility;CRISPR library;CRISPR screen;Custom;Cytometry;Data;Data Collection;Detection;endonuclease;Etiology;experimental study;fitness;Flow Cytometry;Fluorescence Microscopy;functional genomics;Genes;Genetic;Goals;Growth;Immunofluorescence Immunologic;Immunofluorescence Microscopy;in situ sequencing;Individual;inhibitor;Knock-out;Label;Libraries;Measurement;Measures;Methods;Microscopy;Molecular Biology;National Institute of General Medical Sciences;next generation;Organelles;Paint;Pathway interactions;polypeptide;predictive modeling;Proteins;Publishing;Reagent;Research;Resources;response;Running;Science;Seminal;Signal Pathway;Signal Transduction;Structure;System;T-Lymphocyte;Technology;transcriptomics;Wait Time;Work

PROJECT_TITLE: High-content microscopy platform for multiplex perturbation/multiplex readout studies

STUDY_SECTION: MRAA

STUDY_SECTION_NAME: Maximizing Investigators? Research Award A Study Section[MRAA]


NIH_SPENDING_CATS: Genetics;Infectious Diseases;Microbiome

ORG_CITY: STANFORD

ORG_DEPT: GENETICS

ORG_NAME: STANFORD UNIVERSITY

ORG_STATE: CA

PHR: Project Narrative (from Parent R35 Grant) Evolution underlies the emergence of drug resistance in infectious diseases, as well as the progression of cancer. This research will characterize and contrast evolutionary outcomes and their trade-offs from both abiotic and biotic selection pressures. A detailed understanding of such evolutionary outcomes opens the possibility of predicting evolutionary trajectories and improving the design of multi-drug therapy for better patient outcomes.

PI_NAMEs: SHERLOCK, GAVIN J (contact)

PROGRAM_OFFICER_NAME: ADKINS, RONALD

PROJECT_TITLE: Multisizer 4e - equipment for "Fitness Effects of Beneficial Mutations"


ED_INST_TYPE: GRADUATE SCHOOLS

NIH_SPENDING_CATS: Biotechnology;Climate-Related Exposures and Conditions;Infectious Diseases;Social Determinants of Health;Vector-Borne Diseases

ORG_CITY: LAS CRUCES

ORG_NAME: NEW MEXICO STATE UNIVERSITY LAS CRUCES

ORG_STATE: NM

PHR: Project Narrative We request supplemental support for the purchase of a “Micro Clima-Series™ Premium ICH Insect Chamber” from Snijders Labs to further the research capacity of the MVP lab. This machine has unique and specific features that will allow for in-depth investigation on the impact of environmental conditions on mosquito development, adaptation, vectorial capacity, and adult mosquito mating and feeding behaviors. It will enable us to simulate diverse and dynamic diurnal environments with precise changes in temperature, humidity, and lighting.

PI_NAMEs: HANSEN, IMMO ALEX (contact)

PROGRAM_OFFICER_NAME: JUSTINOVA, ZUZANA

PROJECT_TERMS: Adult;Aedes;Biology;Climate;climate change;Culicidae;Development;disease transmission;Environment;Environmental Impact;falls;Feeding behaviors;fitness;Future;Humidity;Insecta;Investigation;Laboratories;lens;Lighting;male;mating behavior;Methods;molecular vector;Mosquito Control;New Mexico;North America;novel;parent project;Physiology;programs;Research;Series;sterile insect technique;Temperature;Universities;vector;vector mosquito;Vectorial capacity

PROJECT_TITLE: The Molecular Vector Physiology Laboratory at New Mexico State University


ADMINISTERING_IC: HL

IC_NAME: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE

ORG_CITY: LOS ANGELES

ORG_DEPT: INTERNAL MEDICINE/MEDICINE

ORG_NAME: UNIVERSITY OF CALIFORNIA LOS ANGELES

PHR: Relevance to Public Health Cardiovascular disease (CVD) is leading cause of death in the United States and represents a significant burden to the national health system. We have uncovered a novel layer of posttranscriptional regulation for LDLR, the determinant of plasma cholesterol and the key driver of CVD, which may result in improved therapeutic interventions.

PI_NAMEs: TARLING, ELIZABETH JOANNA (contact);VALLIM, THOMAS A

PROGRAM_OFFICER_NAME: LIU, LIJUAN

PROJECT_TERMS: 3' Untranslated Regions;Affect;apolipoprotein E-3;atheroprotective;Atherosclerosis;Bile Acid Biosynthesis Pathway;Bile Acids;Binding;Binding Proteins;Binding Sites;Biological Assay;Cardiovascular Diseases;cardiovascular disorder risk;Catabolism;Cause of Death;CETP gene;Cholesterol;cholesterol control;Cholesterol Homeostasis;Clustered Regularly Interspaced Short Palindromic Repeats;Complex;crosslink;crosslinking and immunoprecipitation sequencing;CYP7A1 gene;Data;design;Enzymes;Family;Feedback;Female;functional genomics;gain of function;Genes;Genetic Transcription;Genomic Segment;Health system;Hepatic;High-Throughput Nucleotide Sequencing;Human;Immunoprecipitation;improved;in vivo;Knockout Mice;LDL Cholesterol Lipoproteins;Lipoproteins;Liver;Low-Density Lipoproteins;Luciferases;male;Mediating;Messenger RNA;Metabolic Pathway;Modeling;Molecular;mouse model;mRNA Decay;mRNA Stability;Mus;novel;particle;Pathway interactions;Plasma;Post-Transcriptional Regulation;Post-Translational Regulation;posttranscriptional;Proteins;Public Health;Publishing;Regulation;Reporter;Reporting;Response Elements;Risk Factors;RNA-Binding Proteins;System;Testing;Therapeutic Intervention;Tracer;Transgenes;United States;Untranslated Regions;uptake;Variant;Work

PROJECT_TITLE: Diversity Supplement for “Post-translational regulation of LDLR”


NIH_SPENDING_CATS: Biotechnology

ORG_CITY: LA JOLLA

ORG_DEPT: CHEMISTRY

ORG_NAME: UNIVERSITY OF CALIFORNIA, SAN DIEGO

PHR: PROJECT NARRATIVE Synthetic emissive nucleoside surrogates, combined with powerful real-time fluorescence-based methods, further the fundamental understanding of key biological processes related to disease development. Success would have long-term impact on improving human health by advancing diagnostic tools and facilitating drug discovery.

PI_NAMEs: TOR, YITZHAK  (contact)

PROGRAM_OFFICER_NAME: FABIAN, MILES

PROJECT_TERMS: Articulation;Automobile Driving;Basic Science;Biochemical;Biological Process;biophysical techniques;Chromatography;Circular Dichroism;cofactor;Communities;design;Development;diagnostic tool;Disease;drug discovery;Enzymatic Biochemistry;Enzymes;Event;experimental study;Family;Fluorescence;Funding;Future;Goals;Health;high throughput screening;Human;Image;improved;innovation;Laboratories;Membrane;Metabolism;Methods;Modernization;Monitor;mRNA Decay;multi-photon;Nature;Nucleic Acids;nucleobase;nucleoside analog;Nucleosides;nucleotide analog;Nucleotides;Oligonucleotides;Organelles;Peptidyltransferase;Phase;Play;Process;programs;Research Personnel;Resolution;Role;Screening procedure;Signal Transduction;single molecule;Spectrum Analysis;Structure;success;Techniques;Time;tv watching

PROJECT_TITLE: Fluorescent nucleosides, nucleotides and oligonucleotides


ORG_CITY: CAMBRIDGE

ORG_NAME: MASSACHUSETTS INSTITUTE OF TECHNOLOGY

ORG_STATE: MA

PHR: Project Narrative Enzymes that bind iron–sulfur clusters are found in all kingdoms of life and perform reactions that play central roles in human health, including the generation of ammonia fertilizer from air. We propose to study the chemical bond-making and -breaking steps of these reactions by developing methods for modifying the isotopic and elemental compositions of their catalytic clusters.

PI_NAMEs: SUESS, DANIEL LEIF MIGDOW (contact)

PROGRAM_OFFICER_NAME: ASLAN, KADIR

PROJECT_TITLE: Administrative Supplement for Equipment


PHR: Narrative Not applicable.

PI_NAMEs: QIN, TIAN  (contact)

PROGRAM_OFFICER_NAME: YANG, JIONG

PROJECT_TERMS: Administrative Supplement;Amines;Coupling;Development;Goals;Mediating;Molecular;Pentanes;physical property;programs;Reaction;Research;Research Project Grants;Sulfur;Transition Elements;translational applications

PROJECT_TITLE: Development of Sulfur-Based Reactions with Translational  Applications


NIH_SPENDING_CATS: Biotechnology;Genetics

PI_NAMEs: NAM, YUNSUN  (contact)

PROGRAM_OFFICER_NAME: CZAPLINSKI, KEVIN

PROJECT_TITLE: Mechanisms of Microprocessor Function and Regulation


ORG_DEPT: PHYSIOLOGY

PHR: Project Narrative The importance of circadian clocks in human physiology and mental health is evident from their ubiquitous influence on a wide range of cellular and organismal processes, and the malfunction of the clock is known to be associated with several forms of human psychiatric illness and with sleep disorders. On the other hand, codon usage biases are found in all organisms examined and play an important role in determining gene expression levels and protein structures. A better understanding of these two fundamental mechanisms will potentially lead to better understanding of diverse biological processes and potential therapeutic approaches for treating human diseases.

PI_NAMEs: LIU, YI  (contact)

PROGRAM_OFFICER_NAME: AKACEM, LAMEESE DANA

PROJECT_TERMS: Animals;Behavioral;Biochemical;Biological Phenomena;Biological Process;Cell Physiology;cell type;circadian;circadian pacemaker;Circadian Rhythms;Codon Nucleotides;CSNK1A1 gene;Disease;Drosophila genus;Eukaryota;Frequencies;fungus;Gene Expression;Gene Proteins;Genetic Code;Genetic Screening;Genetic Transcription;Genome;Goals;Human;human disease;in vivo;Mammals;Mental disorders;Mental Health;Methods;Molecular;Mus;Neurospora;novel;Organism;Output;Phosphorylation;Physiological;Physiological Processes;Physiology;Play;Process;protein folding;protein structure;Proteins;Reporter;Role;Sleep Disorders;Speed;System;temporal measurement;Therapeutic;Tissues;Translations;Work

PROJECT_TITLE: Mechanisms of circadian clock and codon usage biases


PHR: This application for administrative supplemental grant is to request fund for purchasing a stage top incubator to enable long time-lapse measurements of dynamic signaling processes in living cells using the confocal-TIRF microscope system in the PI’s lab. This purchase is critical for getting consistent results for proposed research in the parent project.

PI_NAMEs: LIOU, JEN  (contact)

PROGRAM_OFFICER_NAME: CHAO, CHIEN-CHUNG

PROJECT_TERMS: Administrative Supplement;Award;Biological Assay;Calcium Signaling;Carbon Dioxide;Cell membrane;Cells;Endoplasmic Reticulum;Environment;experimental study;Funding;Grant;Homeostasis;Humidity;Image;imaging capabilities;Incubators;live cell imaging;Measurement;Microscope;parent project;Phosphatidylinositol 4,5-Diphosphate;Process;Proteins;quantitative imaging;receptor;Regulation;Research;Signal Transduction;success;System;Temperature

PROJECT_TITLE: Homeostatic Regulation of PIP2-Calcium Signaling


NIH_SPENDING_CATS: Sleep Research

ORG_DEPT: NEUROSCIENCES

PHR: Project Narrative The circadian clock in mammals coordinates many aspects of normal physiology and behavior and when these clocks are disrupted, many health problems, including several types of mental illness, cancer and metabolic problems can occur. This project will investigate how the molecular mechanism of the clock works to keep proper time and drive rhythms in cellular function. This information will be critical for future manipulation of the circadian timing system as a treatment modality for humans suffering from circadian-related health problems.

PI_NAMEs: GREEN, CARLA B. (contact)

PROJECT_TERMS: Behavior;Biochemistry;Carbon Dioxide;Cardiovascular Diseases;Cell Physiology;circadian;Circadian Dysregulation;circadian pacemaker;Circadian Rhythms;confocal imaging;Dedications;Detection;Diabetes Mellitus;Equipment;experimental study;Fluorescence;Fluorescence Polarization;Funding;Future;Gene Expression;Genes;Genetic;Health;high resolution imaging;Human;imaging capabilities;Incubators;instrument;Intracellular Membranes;Jet Lag Syndrome;Length;Link;Location;luminescence;Malignant Neoplasms;Mammals;Measurement;Measures;Mental disorders;Metabolic;Metabolic Pathway;Metabolism;Microscope;Mitochondria;Modality;Molecular;myristoylation;NADP;nocturnin;Nutrient availability;Obesity;Organelles;Periodicity;Phase;Phosphoric Monoester Hydrolases;Physiology;Play;Protein Analysis;protein protein interaction;Reader;response;Role;sensor;shift work;Signal Transduction;System;Temperature;Time;Work

PROJECT_TITLE: Molecular mechanisms of mammalian circadian clock function

STUDY_SECTION_NAME: Special Emphasis Panel[ZRG1(01)-Q]


ADMINISTERING_IC: ES

FUNDING_MECHANISM: NON SBIR/STTR CONTRACTS

IC_NAME: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES

NIH_SPENDING_CATS: Bioengineering;Clinical Research;Health Disparities Research;Health Disparities and Racial or Ethnic Minority Health Research;Patient Safety;Social Determinants of Health

ORG_CITY: SILVER SPRING

ORG_NAME: SOCIAL AND SCIENTIFIC SYSTEMS,  INC.

ORG_STATE: MD

PI_NAMEs: RAMSEY, STEVEN  (contact)

PROJECT_TERMS: abstracting;Advertisements;Advisory Committees;Affect;Age;Agreement;Agriculture;Air;Area;Back;Biological;Blood;Budgets;Carpet;Case Report Form;Case/Control Studies;Certificate of Confidentiality;Certification;Characteristics;Child;Clinic;clinical examination;Clinical Research;Clinical Trials Data Monitoring Committees;Clinics and Hospitals;Code;Collaborations;Collection;Communication;Communities;Compensation;Computer Assisted;Consent;Consent Forms;Constitution;Contractor;Data;Data Analyses;Data Collection;data quality;Death Certificates;design;Development;Diagnosis;dietary;Digit structure;Disasters;Disease;disease classification;Documentation;Dust;Educational workshop;Electronic Mail;Electronics;Eligibility Determination;English Language;Environmental Exposure;equipment acquisition;Expert Opinion;Exposure to;Extramural Activities;Familiarity;Family;Feasibility Studies;Federal Government;Focus Groups;follow-up;foreign language;Future;Gender;Genetic Predisposition to Disease;Geographic Locations;Geography;Goals;Government Agencies;Hand;Health Insurance Portability and Accountability Act;Health Personnel;Hospitals;Human Resources;Human Subject Research;Incentives;Individual;Industrialization;Industry;Institution;Institutional Review Boards;instrument;Interview;Interviewer;invention;Investigation;Labor Unions;Laboratories;Leadership;Letters;literacy;Local Government;Manuals;Measurement;Mediation;Medical;Medical Records;meetings;member;Methods;Migrant;Minority;Monitor;Multicenter Studies;National Institute of Environmental Health Sciences;Newsletter;newspaper article;Nutritional;Occupational;Office Management;operation;Operations Research;Pamphlets;Paper;Participant;Pathologist;Pathology;Pathology Report;Patient Recruitments;Patients;Periodicals;Phase;Physicians;Pilot Projects;Population;posters;Press Releases;Principal Investigator;Privacy;Privatization;Procedures;Prospective Studies;protocol development;Protocols documentation;Proxy;Questionnaires;Race;Rare Diseases;Recommendation;Recording of previous events;Records;recruit;Recruitment Activity;Regulation;Reporting;repository;Research;Research Design;Research Personnel;Research Project Grants;Research Support;Resources;Respondent;response;retention rate;sample collection;Sampling;Scheme;screening;Self Administration;Series;Site;Site Visit;Soil;Specimen;Specimen Handling;Standardization;State Government;Statistical Data Interpretation;Study Subject;Surveys;System;Techniques;Telephone;Testing;Time;Tracer;Training;Training Activity;Training Programs;Translating;Translations;Travel;Underserved Population;United States Food and Drug Administration;United States National Library of Medicine;validation studies;Vital Status;Water;web site;Work

PROJECT_TITLE: TASK ORDER 3R - PROTOCOL DEVELOPMENT AND SUPPORT


ADMINISTERING_IC: FD

IC_NAME: FOOD AND DRUG ADMINISTRATION

ORG_CITY: GENEVE

ORG_COUNTRY: SWITZERLAND

ORG_FIPS: SZ

ORG_NAME: WORLD TRADE ORGANIZATION

PHR: Grant Application Package Cooperative Agreement to Support the Standards and Trade Development Facility (STDF) Project Narrative As a global partnership that assists developing countries to implement international sanitary and phytosanitary (SPS) standards, guidelines and recommendations, the STDF helps to strengthen the capacity of public and private sector stakeholders to implement Codex and other international standards, which contributes to improved domestic public health, as well as market access. More than one-third of STDF projects focus on improving the safety of food for domestic consumption and/or trade. The STDF also raises awareness about the importance of increased capacity to comply with international food safety standards, and the importance of this for public health.

PI_NAMEs: SPREIJ, MELVIN  (contact)

PROGRAM_OFFICER_NAME: ROBINSON, KEVIN

PROJECT_TITLE: Contribution to the Standards and Trade Development Facility (STDF)

STUDY_SECTION_NAME: Special Emphasis Panel[ZFD1 CFSN-T (01)]


ADMINISTERING_IC: AG

IC_NAME: NATIONAL INSTITUTE ON AGING

NIH_SPENDING_CATS: Acquired Cognitive Impairment;Aging;Alzheimer's Disease;Alzheimer's Disease including Alzheimer's Disease Related Dementias (AD/ADRD);Brain Disorders;Clinical Research;Dementia;Disability Research;Estrogen;Menopause;Neurodegenerative;Prevention;Women's Health Research

ORG_CITY: PITTSBURGH

ORG_NAME: UNIVERSITY OF PITTSBURGH AT PITTSBURGH

ORG_STATE: PA

PHR: PROJECT NARRATIVE SWAN-Aging will fill important gaps in understanding the role of the menopausal transition, menopause related health changes and midlife aging on women’s health and functioning in early old age. Accordingly, it will provide useful information to guide clinical decisions in midlife and later life in women who have diverse life experiences and socioeconomic and racial/ethnic characteristics.

PI_NAMEs: BROOKS, MARIA MORI (contact)

PROGRAM_OFFICER_NAME: DUTTA, CHHANDA

PROJECT_TERMS: Activities of Daily Living;Affect;Age;Age Years;age-related disease;Aging;Alzheimer's Disease;Alzheimer's disease related dementia;Ancillary Study;Cardiac health;Cardiovascular Diseases;career development;Characteristics;Clinical;clinical practice;clinical research site;clinically relevant;cognitive function;Communication;Communities;Country;Creativeness;critical period;Data;data centers;Data Collection;Databases;Dementia;dementia risk;design;disability;Doctor of Philosophy;Education;Educational workshop;Enrollment;Ensure;epidemiologic data;Ethnic Origin;Event;follow-up;Fostering;Foundations;Future;Genitourinary system;graduate school;Health;Health Personnel;heart function;human old age (65+);Impaired cognition;Incidence;Infrastructure;innovation;Institution;Institutional Review Boards;Intervention;Investigation;investigator training;Joints;K-Series Research Career Programs;Laboratories;late life;Lead;Leadership;Life;Life Experience;life span;Link;Longitudinal cohort study;Longitudinal Studies;Manuscripts;Measures;Menopause;Methods;middle age;mild cognitive impairment;modifiable risk;Monitor;multi-racial;multiple chronic conditions;Musculoskeletal;novel;Observational epidemiology;Observational Study;operation;organizational structure;Outcome;Participant;participant retention;participant safety;Perimenopause;physical conditioning;Physical Function;physical impairment;physically handicapped;Physiological;preservation;Prevention;Printing;Productivity;psychosocial;Public Health Schools;Publications;Quality of life;Race;Reporting;Research;Research Design;Research Personnel;research study;Research Support;Resources;Risk Factors;Role;Science;Secure;Sex Functioning;Site;Sleep;socioeconomics;Strategic Planning;Structure;Study of Women's Health Across the Nation;success;System;Testing;Translating;Translations;United States;Universities;Vascular Dementia;Visit;welfare;Woman;Women's cohort;Women's Health;Women's study;Work;Writing

PROJECT_TITLE: Core 1: Administrative Core


NIH_SPENDING_CATS: Biodefense and Related Countermeasures;Digestive Diseases;Emerging Infectious Diseases;Foodborne Illness;Infectious Diseases;Microbiome;Nutrition;Rare Diseases

ORG_CITY: CHARLOTTESVILLE

ORG_NAME: UNIVERSITY OF VIRGINIA

ORG_STATE: VA

PHR: The proposed research will determine the molecular mechanisms by which bacteria transport scarce nutrients across their cell membrane. This transport is critical to the functioning of the human microbiome, and it is essential for the success of many bacterial pathogens, such as the bacteria that cause meningitis, cholera and pertussis. Knowledge of these transport mechanisms will assist with the development of new antibiotics to treat bacterial infection.

PI_NAMEs: CAFISO, DAVID S (contact)

PROGRAM_OFFICER_NAME: TAYLOR, VERONICA

PROJECT_TERMS: Antibiotics;Apoptosis;ATP Synthesis Pathway;Bacteria;Bacterial Infections;biophysical analysis;Carbohydrates;Carrier Proteins;Cell membrane;Cell Physiology;Cells;Cholera;Coupling;Crystallography;Development;Dysentery;Electron Spin Resonance Spectroscopy;Electrons;Equilibrium;Escherichia coli;experimental study;Gram-Negative Bacteria;Human Microbiome;In Vitro;Ions;Iron Chelating Agents;Iron Chelation;Island;Knowledge;Lipopolysaccharides;Membrane;Membrane Proteins;Meningitis;Modeling;Molecular;molecular modeling;novel antibiotic class;novel strategies;Nutrient;Osmosis;pathogenic bacteria;Pertussis;Physiologic pulse;protein protein interaction;protein structure;Proteins;Regulation;Research;Resolution;Role;Site;Spin Labels;Structure;success;Testing;Transmembrane Transport;uptake;Vitamin B 12;Work

PROJECT_TITLE: Molecular Mechanisms of Membrane Transport


ADMINISTERING_IC: EY

IC_NAME: NATIONAL EYE INSTITUTE

NIH_SPENDING_CATS: Acquired Cognitive Impairment;Aging;Alzheimer's Disease;Alzheimer's Disease including Alzheimer's Disease Related Dementias (AD/ADRD);Brain Disorders;Dementia;Eye Disease and Disorders of Vision;Neurodegenerative;Neurosciences

ORG_CITY: IRVINE

ORG_DEPT: OPHTHALMOLOGY

ORG_NAME: UNIVERSITY OF CALIFORNIA-IRVINE

PHR: Project Narrative The successful completion of this project will lead to insights into molecular pathways that cause trabecular meshwork dysfunction and elevation of intraocular pressure in glaucoma, a leading cause of irreversible vision loss. The results will provide pathological mechanisms involved in glaucoma and develop novel treatment strategies for glaucoma by targeting these pathologies.

PI_NAMEs: ZODE, GULAB  (contact)

PROGRAM_OFFICER_NAME: SONG, HONGMAN

PROJECT_TERMS: Autophagocytosis;Autopsy;Biological Assay;Blindness;Cell Death;Cell model;Cell Physiology;Cells;Cessation of life;Chronic;Communication;Deposition;Disease;Endoplasmic Reticulum;endoplasmic reticulum stress;Enhancers;Exhibits;Extracellular Matrix;Eye;Eyedrops;Fibrosis;Functional disorder;Funding;Genetic;Genus Hippocampus;Glaucoma;Glucocorticoids;Goals;Health;Homeostasis;Human;Impairment;improved;In Vitro;in vivo;in vivo Model;insight;Link;Lysosomes;Membrane;Metformin;Mitochondria;mitochondrial dysfunction;Molecular;mouse model;Mus;Nerve Degeneration;Normal tissue morphology;novel;Ocular Hypertension;Organelles;Pathologic;Pathology;Pathway interactions;pharmacologic;Physiologic Intraocular Pressure;Play;prevent;Primary Open Angle Glaucoma;Proteins;Reactive Oxygen Species;Reporter;Role;Stress;Time;Tissues;Trabecular meshwork structure;transcription factor;Transforming Growth Factor beta;Transmission Electron Microscopy;treatment strategy;Work

PROJECT_TITLE: Crosstalk between chronic ER stress and mitophagy for the treatment of POAG

STUDY_SECTION_NAME: Pathophysiology of Eye Disease - 1 Study Section[PED1]


NIH_SPENDING_CATS: Antimicrobial Resistance;Infectious Diseases;Machine Learning and Artificial Intelligence

ORG_CITY: Nashville

ORG_NAME: VANDERBILT UNIVERSITY

ORG_STATE: TN

PHR: Project Narrative of Funded R35 (GM152382): Structural Dynamics of Active Transporters. Steady emergence of microbes displaying acquired drug resistance phenotypes has prompted health organizations to declare multidrug resistance a global public health concern. In addition, multidrug resistance compromises the efficacy of cancer treatment. The proposed studies will yield a structural dynamic blueprint for transporters underlying basic multidrug resistance phenomena in bacteria and humans, knowledge which can be exploited to develop strategies to inhibit these transporters.

PI_NAMEs: MCHAOURAB, HASSANE S (contact)

PROJECT_TERMS: acquired drug resistance;Address;ATP Hydrolysis;Bacteria;Bacterial Infections;Binding;Binding Sites;cancer therapy;Cell membrane;Cells;Clinical;Coupled;Coupling;Cryoelectron Microscopy;cytotoxic;Drug resistance;efflux pump;Electrons;Elements;Environment;Family;Free Energy;Funding;Goals;health organization;Human;innovation;Investigation;Ions;Isomerism;Knowledge;Lipid Bilayers;Lipids;Machine Learning;Mediating;Membrane;Microbe;Molecular Conformation;Multi-Drug Resistance;Mycoses;neoplasm chemotherapy;overexpression;Pharmaceutical Preparations;Phenotype;Productivity;programs;protein structure prediction;Public Health;Research;Resolution;Shapes;solute;spectral energy;Spectrum Analysis;Structure;Tertiary Protein Structure;tool;Toxin


NIH_SPENDING_CATS: Brain Disorders;Fragile X Syndrome;Genetics;Intellectual and Developmental Disabilities (IDD);Neurosciences;Rare Diseases

ORG_CITY: WORCESTER

ORG_DEPT: ANATOMY/CELL BIOLOGY

ORG_NAME: UNIV OF MASSACHUSETTS MED SCH WORCESTER

PHR: We propose to employ a Luminex 200 system for multiplexed quantification of proteins and RNAs, especially CPEB and FMRP, which both control RNA expression and regulate neural function. Because ablation of CPEB and FMRP lead to neurodevelopmental disorders including autism and Fragile X Syndrome, our work is directly related to human health.

PI_NAMEs: RICHTER, JOEL D (contact)

PROGRAM_OFFICER_NAME: VATAKIS, DIMITRIOS NIKOLAOS

PROJECT_TERMS: 3' Untranslated Regions;Ablation;Affect;Alternative Splicing;Animal Behavior;Area;autism spectrum disorder;Behavioral;Binding;Brain;Cells;Chromatin;Codon Nucleotides;Cognition;Cytoplasm;Electrophysiology (science);Epigenetic Process;Event;Excision;experimental study;FMR1 gene;Fragile X Syndrome;Functional disorder;Genes;Genetic Transcription;Growth;Health;Human;Knockout Mice;Laboratories;Lead;Learning;Length;Link;Mediating;Memory;Messenger RNA;Minority;Molecular;mouse model;mRNA Precursor;mRNA Translation;Mus;nanopore;Neurodegenerative Disorders;Neurodevelopmental Disorder;neuropathology;Neurophysiology - biologic function;neuroregulation;Nucleotides;Poly(A) Tail;Polyadenylation;Post-Transcriptional Regulation;postsynaptic;Protein Isoforms;Proteins;Regulation;Research;Resolution;ribosome profiling;Ribosomes;RNA;RNA Degradation;RNA Splicing;RNA Stability;RNA-Binding Proteins;Synapses;synaptic function;Synaptic plasticity;System;transcriptome sequencing;Translation Initiation;Translational Regulation;Translations;Work

PROJECT_TITLE: Acquisition of a Luminex Multiplex Platform


ORG_CITY: ANN ARBOR

ORG_NAME: UNIVERSITY OF MICHIGAN AT ANN ARBOR

ORG_STATE: MI

PHR: Leveraging a portfolio of long-standing expertise at the interface of RNA biology and single molecule biophysics, the Walter group applies advanced mechanistic enzymology approaches to a broad set of RNAs involved in the regulation of transcription, translation and splicing, thus seizing the opportunities that continue to arise from discoveries of new RNA functionalities. In addition, we will push the limits of our single molecule approaches to probe increasingly complex biological contexts and mechanisms in which RNA structure governs the function of the cellular gene expression machinery. The integration of the proposed versatile, automated, turnkey and easy- to-use AbbeLight SAFe M90 imaging module into our existing microscope will dramatically increase the throughput, precision, and multi-color capabilities of our discovery pipeline, allowing beginning postdocs, graduate students and undergraduate students alike to quickly climb the learning curve to acquire rich datasets that elucidate miRNA-guided intracellular gene silencing pathways.

PI_NAMEs: WALTER, NILS G (contact)

PROJECT_TERMS: 15 year old;Acceleration;Address;Administrative Supplement;Automation;Award;Biological;Biology;Biophysics;Cell Physiology;Cells;Color;Complement;Complex;Data Set;Enzymatic Biochemistry;Fluorescence;fluorescence imaging;fluorescence microscope;fluorophore;Funding;Gene Expression;gene function;Gene Silencing Pathway;Genetic Transcription;graduate student;Grant;Image;Imaging Device;Individual;Injections;instrument;instrumentation;Investments;Label;Lead;Learning;Mammalian Cell;Measurement;Messenger RNA;Methods;MicroRNAs;Microscope;nanomachine;nanoscale;Optics;Outcome;parent grant;Parents;particle;Performance;Plug-in;Postdoctoral Fellow;Proteins;Readiness;Regulation;Resolution;RNA;RNA Splicing;Sampling;single molecule;spatiotemporal;Spliceosomes;Structure;Students;Technology;Time;Transcriptional Regulation;Translations;undergraduate student;United States National Institutes of Health;Untranslated RNA;Update;Yeasts

PROJECT_TITLE: Administrative Supplement for AbbeLight SAFe M90 automated, multicolor, optically perfect TIRF imaging: The RNA nanomachines of the gene expression machinery dissected at the single molecule level


NIH_SPENDING_CATS: Aging

ORG_CITY: SYRACUSE

ORG_NAME: UPSTATE MEDICAL UNIVERSITY

ORG_STATE: NY

PHR: NARRATIVE OF PUBLIC HEALTH RELEVANCE  V-ATPases are present in all cells and help control pH in specific sub-compartments, degrade defective proteins, and respond to environmental stresses. V-ATPases are remarkably conserved. Work in the yeast model system has revealed central mechanisms of regulation, such as reversible disassembly of the enzyme, as well as cellular functions dependent on V- ATPase function, including lysosomal function and aging. Many of our experiments depend on wide-field microscopy to trace localization of V-ATPase subunits and key regulators in both yeast and mammalian cells.

PI_NAMEs: KANE, PATRICIA M (contact)

PROGRAM_OFFICER_NAME: ANSONG, CHARLES KWAKU

PROJECT_TERMS: Administrative Supplement;Age;Aging;Alkalinization;Caloric Restriction;cell age;Cell Physiology;Cells;Complex;Computer software;environmental stressor;Enzymes;Eukaryotic Cell;experimental study;extracellular;Fluorescence Microscopy;Glucose;Image;improved;life span;Location;Mammalian Cell;Membrane;Microscope;Microscopy;operation;Organelles;Organism;Parents;Peripheral;Play;Process;Protein Isoforms;Proteins;Proton Pump;public health relevance;Regulation;Role;Signaling Protein;Update;vacuolar H+-ATPase;Vacuole;virtual;Visualization;Work;Yeast Model System;Yeasts

PROJECT_TITLE: Regulation and Cellular Functions of V-ATPases


ADMINISTERING_IC: AI

IC_NAME: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES

NIH_SPENDING_CATS: Biodefense and Related Countermeasures;Clinical Research;Clinical Trials and Supportive Activities;Emerging Infectious Diseases;HIV/AIDS;Infectious Diseases;Lung;Orphan Drug;Patient Safety;Pediatric;Pediatric - AIDS;Rare Diseases;Tuberculosis

ORG_CITY: Durban

ORG_COUNTRY: SOUTH AFRICA

ORG_FIPS: SF

ORG_NAME: CENTRE/AIDS PROGRAMME/RES/SOUTH AFRICA

PHR: Project Narrative: Integrase stand transfer inhibitors (InSTI), in particular dolutegravir and bictegravir, are potent, safe, well- tolerated drugs used for the treatment of HIV infection, but we do not yet fully know how to use them in patients with HIV and tuberculosis. Drug interactions with rifampicin, a key first-line tuberculosis drug, reduces exposures to these drugs and may reduce their efficacy, so we will therefore study whether increasing the doses of these drugs can overcome drug interactions and will be safe and effective for the treatment of HIV infection. Specifically, we will examine drug exposures, safety and efficacy of dolutegravir when given twice daily (double dose) to children (20-35kg) with HIV and TB on rifampicin to provide an evidence-base for the use of this drug in this subpopulation, and we will test twice-daily bictegravir/ /emtricitabine/tenofovir alafenamide (BIC/TAF/FTC) in adults with HIV taking tuberculosis treatment to ensure drug concentration and efficacy targets are met.

PI_NAMEs: DOOLEY, KELLY E.;NAIDOO, ANUSHKA  (contact)

PROGRAM_OFFICER_NAME: LOMBO RODRIGUEZ, TANIA

PROJECT_TERMS: ABCB1 gene;Adult;Africa;Africa South of the Sahara;antiretroviral therapy;Area;Child;Clinical;clinically relevant;co-infection;CYP3A4 gene;Data;Dedications;Diphosphates;Dose;Drug Exposure;Drug Interactions;Drug Kinetics;Drug usage;efavirenz;effective therapy;efficacy evaluation;emtricitabine;Enrollment;Ensure;Enzymes;Epidemic;evidence base;Exposure to;Film;Formulation;Fumarates;Future;Generations;Genetic;genetic testing;Goals;healthy volunteer;HIV;HIV Infections;HIV Seronegativity;HIV Seropositivity;HIV-1;HIV/TB;Infection;inhibitor;Integrase;Investigation;Knowledge;Laboratories;Lamivudine;Measures;Medical;mortality;Patients;pediatrician;Persons;Pharmaceutical Preparations;phase III trial;pill;Plasma;Positioning Attribute;Price;Protease Inhibitor;Proteins;Publishing;Qualifying;Randomized;Recommendation;Regimen;Research;Resistance;Resistance development;Resource-limited setting;Rifampin;Safety;Sampling;South Africa;standard of care;Tablets;Tenofovir;Testing;Treatment Protocols;treatment research;Tuberculosis;tuberculosis drugs;tuberculosis treatment;UGT1A1 gene;Viral;Viral Load result;Weight;World Health Organization

PROJECT_TITLE: Second Generation InSTIs for the Treatment of HIV-1 in patients with TB co-infection on Rifampicin-based Treatment in KwaZulu Natal, South Africa


NIH_SPENDING_CATS: Natural Products

ORG_CITY: CHESTNUT HILL

ORG_NAME: BOSTON COLLEGE

PHR: PROJECT NARRATIVE Catalytic Processes for Stereoselective Radical Cyclization Reactions  In modern pharmaceutical industry, where the market share of single enantiomer chiral drugs continues to rise, there are growing demands for developing practical and economical methods for the enantioselective synthesis of biologically and pharmaceutically important carbocyclic and heterocyclic compounds in an enantioenriched or enantiopure form. Compared to a racemic mixture, the use of an enantiomerically pure compound improves the desired bioactivity and while reducing toxicity because enantiomeric isomers often exhibit different biological activities. For drug discovery and development, it is desirable to prepare both enantiomers of a new chiral drug candidate for testing their separate activities.

PI_NAMEs: ZHANG, X. PETER  (contact)

PROJECT_TERMS: Alkenes;Azides;Benign;Biological;Catalysis;Chemistry;Cobalt;Complex;cost effective;covalent bond;Cyclization;Development;diazo compound;Dissociation;drug candidate;drug development;drug discovery;Drug Industry;Electronics;enantiomer;Environment;Exhibits;functional group;Generations;Heterocyclic Compounds;Hydrogen;improved;Isomerism;Ligands;Marketing;Methods;Modernization;Molecular;Molecular Structure;Natural Products;Nature;novel strategies;Periodicity;Pharmaceutical Preparations;Play;Porphyrins;Process;Reaction;reaction rate;Research;small molecule;Solvents;Structure;Testing;Toxic effect

STUDY_SECTION: SBCB

STUDY_SECTION_NAME: Synthetic and Biological Chemistry B Study Section[SBCB]


NIH_SPENDING_CATS: Antimicrobial Resistance;Biodefense and Related Countermeasures;Emerging Infectious Diseases;Infectious Diseases

ORG_CITY: ATLANTA

ORG_DEPT: PHYSICS

ORG_NAME: GEORGIA INSTITUTE OF TECHNOLOGY

ORG_STATE: GA

PHR: Narrative The threat posed by Gram-negative bacteria is significant and growing due to the spread of antibiotic resistance. Infection by these pathogens is often mediated by virulence factors known as autotransporter proteins. This project will determine the atomic-level details of autotransporter folding and expression, thus providing a basis for novel antibacterial interventions.

PI_NAMEs: GUMBART, JAMES C. (contact)

PROGRAM_OFFICER_NAME: GERSHENSON, ANNE

PROJECT_TERMS: Administrative Supplement;Anti-Bacterial Agents;Antibiotic Resistance;Bacterial Adhesins;beta barrel;Cell surface;Cessation of life;Chemicals;Collaborations;deep learning model;Environment;Equipment;Exhibits;Extracellular Space;Goals;Grain;Gram-Negative Bacteria;Gram-Negative Bacterial Infections;Hybrids;In Vitro;in vivo;Infection;Infection Control;innovation;Intervention;Kinetics;laboratory experiment;Mediating;member;Membrane;Methodology;Modeling;Molecular;molecular dynamics;molecular scale;novel;pathogen;Pathogenicity;Pathway interactions;Peptide Hydrolases;priority pathogen;Process;Proteins;Research;Resistance development;Side;simulation;System;tool;Validation;Virulence Factors;World Health Organization

PROJECT_TITLE: Administrative Supplement Equipment Request

STUDY_SECTION: BBM

STUDY_SECTION_NAME: Biochemistry and Biophysics of Membranes Study Section[BBM]


ADMINISTERING_IC: MD

IC_NAME: NATIONAL INSTITUTE ON MINORITY HEALTH AND HEALTH DISPARITIES

NIH_SPENDING_CATS: American Indian or Alaska Native;Asthma;Basic Behavioral and Social Science;Behavioral and Social Science;Clinical Research;Endocrine Disruptors;Health Disparities Research;Health Disparities and Racial or Ethnic Minority Health Research;Infectious Diseases;Lung;Pediatric;Prevention;Racial and Ethnic Minority Health Research;Rural Health;Social Determinants of Health

ORG_CITY: EAGLE BUTTE

ORG_NAME: MISSOURI BREAKS RESEARCH, INC.

ORG_STATE: SD

PHR: C. Career Development and Mentorship Plan Career and Research Goals: Dr. Spear’s overall career goal is to become a leading investigator focused on clinical translational studies and drug development, including the integration of environmental contributions to health outcomes in the context of human genetic diversity. She plans to build leadership skills and hands on experience in topics of environmental health to support her career development, which has thus far been focused on genetics. Dr. Spear has extensive training in computational biology for which she has applied to understanding the genetic architecture of biomedical traits in underrepresented populations. However, moving forward her goal is to engage in community- based research that is more centered on environmental considerations, which is highly complementary to the specific aims of the parent award. Thus, Dr. Spear’s career development plan has been intentionally developed for her to obtain new experiences and skill sets to extend her knowledge of genomic data analyses into environmental aspects of human health, and to engage with bi-directional community-driven research. Specifically, she will investigate the contribution of environmental toxicants (diversity supplement) in the context of social stress (parent award) on the immune function and response to viral respiratory function of children with asthma. A diversity supplement will provide Dr. Spear with the necessary structure for her to become a leader in the field, and acquire mentorship and experiences that are relevant to this stage in her career. Mentorship Plan The Principal Investigators of the parent grant are deeply committed to mentoring and supporting the scientific and career development of Dr. Spear. Dr. Spear’s primary mentor will be Dr. Torgerson, who brings expertise as it relates to statistical considerations and methods for environmental and epidemiological studies in asthma, and responsible conduct in human subjects research. She also has longstanding experience in working with the community including outreach activities, and will provide mentorship on responsible conduct in research and career development programs at UCSF. Dr. Best is a physician scientist and will provide mentorship with regards to clinical and administrative considerations for community-based public health research involving American Indian individuals and communities. This includes special considerations for Human Subjects Research in a vulnerable, underserved community, given his knowledge and experience of longstanding collaborations with American Indian communities and health care systems. Dr. Erdei will provide mentorship with respect to studies of environmental toxicants, including aspects related to environmental justice, and has specific experience in studies of metal and PFAS exposure in American Indian communities including the Cheyenne River Sioux Tribe and Navajo. Together, we have assembled a complementary mentorship team to ensure that Dr. Spear will have input from diverse fields of expertise and perspectives, as she takes on an independent leadership role and acquires new experiences specific to human subjects research and environmental studies. Dr. Torgerson will meet individually with Dr. Spear every week to support her scientific and career development as she develops her independent research career in biomedical research. The agenda will generally include a 5-10 minute check-in, followed by discussion of specific scientific developments and progress/roadblocks as facilitated by Dr. Spear. The last 10 minutes will be devoted to jointly reviewing benchmarks and timelines for career development activities as outlined in this proposal. Dr. Spear will present quarterly in Dr. Torgerson’s all-member lab meetings, and attend as desired, and have the option to participate in a weekly Journal Club focused on select topics of interest. Dr. Spear will also join weekly research meetings devoted to the FIPA2 study, and including her additional mentors Dr. Best and Dr. Erdei, FIPA2 study coordinators, staff, and personnel involved in the planning and recruitment of participants. Should the diversity supplement be awarded, Drs. Best and Erdei will meet individually with Dr. Spear at least quarterly for individualized mentoring opportunities and check- ins for scientific and career support. Dr. Spear will also travel to MBIRI in Eagle Butte and Rapid City 2- 3 times annually to interact with the research team in person, and participate in study recruitment, work with study coordinators, interact with members of the community, present her research to the Community Advisory Board and Health Committees for feedback, and participate in community dissemination and outreach events. Career Development Plan Dr. Spear’s proposed research and career development plan will allow her to acquire training and first hand experiences from early stages of community based research, spanning study design to regulatory approvals, study recruitment and ongoing engagement with the community. She will develop more insight into environmental and human subjects research - from conception to recruitment to data generation, while receiving mentorship in areas of building community relationships in underserved communities, navigating the IRB process, budget management, sample processing and handling, and data coordination. She will travel to Rapid City and Eagle Butte to participate and observe in study recruitment and engage with the community both informally and through organized events including meetings with the existing Community Advisory Board to participate in bi-directional learning. She will participate in activities with the EaRTH Center at UCSF and obtain skills and intuition for integrating environmental measures into studies of human health. Continuing to refine her leadership abilities will be supported through practicing her written and oral communication skills at presentations at community events and scientific meetings, and the preparation of first author and collaborative manuscripts. Dr. Spear will also benefit from formal training opportunities in clinical research at UCSF that are relevant to her career stage. This includes courses and workshops offered through the Department of Epidemiology and Biostatistics to gain expertise in clinical research studies, including methods for community engagement (e.g. developing budgets for community engagement, paying community advisors, developing research and evaluation literacy for community agencies, and dissemination and media training for researchers), a summer clinical research workshop, a course in responsible conduct of research, and designing clinical research course at UCSF. In addition, Dr. Spear will acquire new skills and experiences through real world experiences and engaging with the community at our two study sites in Eagle Butte SD and Rapid City ND. This will include engaging with the Tribal Health Committee and Great Plains IRB, attending and participating in the organization of community outreach events (e.g. community events at MBIRI, school outreach activities, radio broadcasts, pamphlet development), and spending time assisting the recruitment of study participants through working with our clinical coordinators who are active at both sites. Lastly, she will lead the dissemination of her research through social media, peer-reviewed scientific publications, and presentations at scientific meetings including the American Thoracic Society (ATS) and Pediatric Academic Societies (PAS). Timeline and Detailed Activities:  Year 1 9/1/24-7/31/25  Year 2 8/1/25-7/31/26  Q 2 Q 3 Q 4 Q 1 Q 2 Q 3 Q 4 Skills development  UCSF Clinical and Translational Science Institute - Sessions on Community Engagement x  Responsible Conduct of Research Course for Clinical Researchers x  Summer Clinical Research Workshop at UCSF – Introduction to Clinical Research x  Designing Clinical Research Course x  Environmental Justice Bootcamp at Harvard University: Theory and Methods to Study Environmental Health Disparities (2-day course) x  Research and Career Development  Engage with Tribal Health Committee and Great Plains IRB x x  Present Research in Community Forums (e.g. community event at MBIRI, radio broadcast) x x x  Pamphlet and promotional materials development x x  Work with Clinical Coordinators in participant recruitment x x x  Present Results to the EaRTH Center at UCSF x x  Manuscript writing and publication x x x x Attend and present at annual meetings of the American Thoracic Society Annual Meeting and Pediatric Academic Societies x x Attend Career Fairs and Recruitment Events x x x x Preparation of CV and Job Applications x x

PI_NAMEs: BEST, LYLE G (contact);ERDEI, ESTHER ;TORGERSON, DARA

PROGRAM_OFFICER_NAME: BICKHAM-WRIGHT, UTIBE RONALD

PROJECT_TERMS: 17 year old;2019-nCoV;Acids;Address;Adult;adverse childhood events;Age;Air Pollution;American;American Indian community;American Indian Population;American Indians;Antigens;Antinuclear Antibodies;Area;Asthma;asthma prevention;atopy;Autoimmune;Autoimmune Responses;Award;behavior influence;Benchmarking;Biological;Biological Assay;Biological Markers;Biomedical Research;Biometry;Body mass index;Bronchiolitis;Budgets;career;career development;career networking;Case/Control Studies;Chemical Exposure;Chemicals;Chest;Cheyenne;Child;Child Health;Childhood;Childhood Asthma;Cities;Clinical;Clinical Research;Clinical Sciences;clinical translation;Cluster Analysis;Collaborations;Communities;community advisory board;community based research;community building;community engaged research;community engagement;Community Healthcare;community organizations;Community Outreach;Complex;Computational Biology;Conceptions;Consultations;Consumption;Core Facility;County;cytokine;Data;Data Analyses;Dedications;design;Development;Development Plans;Diagnosis;dietary;Discriminant Analysis;Disparity;DNA;drug development;early experience;Educational workshop;Emergency department visit;Environment;Environmental Health;environmental health disparity;environmental justice;Environmental Risk Factor;eosinophil;Epidemiology;epidemiology study;Ethnic Population;Evaluation;Event;experience;Exposure to;Feedback;Fetal Growth;Fishes;follow-up;Frequencies;Funding;Future;Gender;Generations;Genetic;genetic architecture;Genetic Variation;genomic data;Goals;Great Plains;Health;health disparity;Healthcare Systems;high body mass index;High Prevalence;Hospitalization;Household;Housing;Human;Human Genetics;Human Resources;Human Subject Research;IgE;Immune;immune function;Immune response;Immune system;Immune System Diseases;Immunoglobulin G;Immunoglobulin M;Immunoglobulins;Immunologic Markers;Immunologics;Immunosuppression;Individual;Indoor Air Quality;Infant;Infection;Inflammation;Influenza;Insecta;insight;Institutional Review Boards;interest;Intervention;Interview;Intuition;Investigation;Job Application;Journals;Knowledge;Leadership;Learning;Least-Squares Analysis;Life;Linear Regressions;Link;literacy;Location;Logistic Regressions;Low income;Manuscripts;Measurement;Measures;Mediation;meetings;member;Mentors;Mentorship;Metals;Methods;Modeling;National Institute on Minority Health and Health Disparities;Native American community;Navajo;neglect;Northern Plains;Not Hispanic or Latino;offspring;oral communication;Outcome;outreach;Pamphlets;parent grant;parent project;Parents;Participant;pathogen;Patient Recruitments;Pattern;Peer Review;Persons;Pharmaceutical Preparations;Physical environment;Physicians;Planet Earth;Play;Policies;Poly-fluoroalkyl substances;Poverty;predictive modeling;Predisposition;Pregnancy;Preparation;Prevalence;Preventive measure;Principal Component Analysis;Principal Investigator;Process;process evaluation;Program Development;Proteins;Protocols documentation;public health research;Publications;Questionnaires;racial population;Radio;Recontacts;recruit;referral services;Reporting;Research;research and development;Research Design;Research Personnel;research study;Reservations;residence;respiratory;respiratory health;Respiratory physiology;Respiratory Signs and Symptoms;Respiratory syncytial virus;Respiratory Syncytial Virus Infections;response;responsible research conduct;Rhinovirus;Risk;ROC Curve;Rodent;Role;Rural;Sampling;Sampling Studies;Schools;Scientist;screening;Seasons;Serology;Serum;sex;Sioux Indians;Site;skill acquisition;skills;social;social determinants;social factors;social influence;social media;social stress;Societies;Source;South Dakota;Statistical Data Interpretation;Stress;stressor;Structure;Surveys;Symptoms;Testing;Teton Sioux Indian;theories;Thyroglobulin antibody;Thyroid Gland;Time;timeline;Tissues;Tobacco smoke;Toxic Environmental Substances;Training;training opportunity;trait;transition to adulthood;Translational Research;translational study;Travel;tribal community;tribal health;tribal member;Tribes;Underrepresented Populations;underserved community;Universities;Vaccines;Viral;Viral Respiratory Tract Infection;volunteer;Water;White Blood Cell Count procedure;Work;Writing

PROJECT_TITLE: Factors Influencing Pediatric Asthma into Adulthood (FIPA2)


ORG_CITY: DURHAM

ORG_NAME: DUKE UNIVERSITY

ORG_STATE: NC

PHR: PROJECT NARRATIVE The ubiquitous presence of nitrogen atom in small-molecule probes and drugs highlights the significance of nitrogen-containing molecules in biomedical research and drug discovery. This project will develop new catalytic methods for regio- and stereoselective aminofunctionalization of alkenes, especially those involving C–N bond formation cascade reactions, to advance the synthesis and study of novel nitrogen-containing molecules. Such ability to prepare structurally diverse nitrogen-containing skeletons in the most rapid and efficient way possible is of the utmost importance, as is the ability to understand their biology, pharmacology and potential as new leads and tool compounds.

PI_NAMEs: WANG, QIU  (contact)

PROJECT_TERMS: Alkenes;Amination;Biological;Biology;Biomedical Research;Chemicals;Chemistry;Complex;cost efficient;design;Development;drug candidate;drug discovery;Engineering;Goals;human disease;invention;Lead;Methods;Molecular Structure;Nitrogen;novel;novel therapeutics;Organic Synthesis;Pharmaceutical Chemistry;Pharmaceutical Preparations;Pharmacology;Process;Reaction;Research;Skeleton;small molecule;Technology;Therapeutic;tool

PROJECT_TITLE: New Methods for the Synthesis and Study of Bioactive Nitrogen-Containing Molecules


ORG_CITY: CHAPEL HILL

ORG_NAME: UNIV OF NORTH CAROLINA CHAPEL HILL

PHR: Simon J. Meek: New Organoboron-Based Methods and Strategies for Organic Synthesis; 5R35GM141558 Research Strategy: A. Summary:  The efficient and stereoselective preparation of chiral organic molecules is vitally important to the development of new therapeutic agents. Consequently, development of new transformations that provide synthetic chemists with strategic bond disconnections that facilitate de novo approaches to organic synthesis is imperative. Research under the aegis of this MIRA grant broadly seeks to develop and exploit new, practical, and efficient stereoselective technologies for organic synthesis of molecular scaffolds of biological importance that are otherwise difficult to access or inefficiently prepared via current processes. To this end, we are interested in developing transformations that leverage the broad chemical reactivity of multi-functional chemical synthons for complex molecule synthesis. These readily accessible platforms merge powerful bond forming reactivity with broad synthetic utility, to deliver versatile organic compounds of notable significance for fragment-based drug discovery. Building off our previous work, we are developing new catalytic C–C, C–N, and C–X bond forming methods for the synthesis of chiral amines, alcohols, and aromatic structures, with a particular focus on examples that contain quaternary carbon stereogenic centers. For example, chiral metal nucleophiles can be accessed by enantioselective transmetalation of readily accessible achiral multifunctional reagents. The resultant nucleophilic species can engage in a number of enantioselective reactions with various C=O, C=N, C–X electrophiles, to deliver non-racemic versatile organic compounds of notable significance. Moreover, we are targeting the generation and utility of novel functionalized synthons by metal-free Lewis base catalysis for reactions with electrophiles that are difficult to achieve via transition metal catalysis. Importantly, enantioselective variants of these unknown reactions are being explored. Stereoselective reactions currently under study are shown in Schemes 1 and 2. Scheme 1. Catalytic Stereo- and Site-selective Reactions with New Multifunctional Reagents:  A. General Reaction Theme: B. Program: Stereoselective Synthesis with Multifunctional Reagents  (chiral) · Representative Example: stereo-  FG1 FG2 catalyst chiral R2 NHG controlled R2 NHG  NG B(pin)  FG3 FG4 catalyst R1 R3 transformation R1  R3  R1 R2 (pin)B R3 B(pin) G = C, O,  G  simple accessible Complex N, X  multifunctional Functionalized high dr, er  building blocks products ■ Convergent Stereoselective Complexity Building Reactions Synthons:  R1 B(OR)2  R2 α-Boryl carbanion  R1 B(OR)2  R2  α-Boryl radical C. Examples of Challenging Molecular Scaffolds · Quaternary Carbons, N  Vicinal Stereocenters  – Challenging Me H OMe  O Me  Me  Me OH · Chiral Amines  H  – Common motifs  arenol A in drugs  HO D. Representative Copper-Catalyzed Enantioselective Synthesis of Tertiary Alcohols and Amines By C–O and C–N Bond Formation:  Me ■ Representative Examples: [(pin)B]2HC OH OH  R CuOtBu (10.0 mol%)  1 (1.0 equiv) L1 (12.0 mol%) B(pin) B(pin) + Me Me  Ph CD3OD (1.05 equiv) Me N THF, -60 °C, 16 h 4 5  N N or N 72% yield, >98% yield, Me O CO2Me 91:9 e.r. 97:3 e.r. CO2Me  Ph N B(pin)  N  H Me nBu  6  62% yield  88:12 e.r. Ar=3,5-Me2C6H3  L1 2 (1.0 equiv) 3 (in situ N–O cleavage) Ar O  P Me O Ar R1 B(pin) CuL* X  N R2 7  N Me  Me  Enantioselective Synthesis of Tertiary Alcohols and Amines: As illustrated in Scheme 2D, we are investigating the enantioselective Cu-catalyzed synthesis of tertiary allylic alcohols and amines. Treatment of catalytically generated enantioenriched copper-allyl with nitroso–arenes and diazene electrophiles furnishes tertiary alcohols and amines in high yield and enantioselectivity. Alkoxy–amine intermediates undergo rapid N– O cleavage with a second equivalent of nitroso-arene to yield alcohols 4 and 5 in high yield and enantioselectivity. Reactions are promoted by chiral phosphoramidite-copper complexes that lead to >98:2 a:g, >91:9 e.r. In addition, the N–O intermediates can be intercepted prior to N–O cleavage.  Stereoselective Defluoro-Allyl addition Methods and Reactions with Boryl-Enamines: We have harnessed the reactivity afforded by boron-stabilized carbanions to establish a method for the site- and stereoselective C– C couplings of aryl–CF3 and –CF2 groups with allylic nucleophiles (e.g., 10 and 12). Moreover, we have discovered a powerful method for the synthesis of borylated enamines (Scheme 2). These are versatile intermediates that are typically difficult to prepare for a range of amines but provide an avenue for amine incorporation through various coupling reactions. Examples of enantioselective transformations and reaction that generate stereocenters include, enantioselective conjugate addition (15), three-component of formamides into a-amino nitriles (16) (amino acid precursors), and efficient Pd-catalyzed cross-coupling followed by reduction to afford chiral trifluoromethyl alcohols (17). These C-C bond forming reactions are being optimized and their enantioselective variants will be evaluated. Scheme 2. Representative Examples of Lewis Base Promoted Site- and Stereoselective Defluoro–Allyl Additions of Trifluoromethyl- and Difluoromethylene-Arenes:  · Diastereoselective Crotylation: Synthesis of Tertiary–Alkyl Fluorine Stereocenters · Diastereoselective Sequential Defluoro-Allyl Additions to Trifluoromethyl–Arenes:  F F  B(pin) 1) 10 mol % CsF, 18-Cr-6, allyl-SiMe3 F  F CF3 DME, 80 °C, 18 h  + CH[B(pin)]2 LiOMe (1.5 equiv) B(pin)  N Me Me 10  9 N Ph N 2) LiOMe (1.5 equiv), allyl-[B(pin)]2 Me nBu  8 DME, 80 °C, 18 h Ph N  DME, 80 °C, 18 h (1.0 equiv.) (1.5 equiv.) 73% yield 11 12  r.r. = >95:5 ? :?  7:1 dr 43% yield (2 steps), 3:1 d.r. F. Representative Synthesis and Reactivity of Boryl Enamines and Amine Substituted Allyl Boronates: ■ Synthetic Approach: R  O B(pin)  Li–CH[B(pin)]2 N  N R  14  13  Boryl Enamine  Amides – Ubiquitous  (Versatile Intermediate)  Functional Group · Enantioselective 1,4-Addition: · Three-Component Coupling: · Trifluoromethyl Acetone Coupling: O –hydrocyanationPh  –Hydrolyysis and reduction  O MeO MeO  N Ph OH  N OtBu C CF3  H N  15 Ph 16 17 85% yield, >20:1 E/Z, 96:4 e.r. 33% yield (3 steps) 89% yield (2 steps) B. Justification of Need: Replacement for a broken SFC instrument and critical need for efficient chiral SFC analysis of difficult to separate enantiomers of alcohols, amines, and fluorine-containing stereocenters.  A critical aspect of catalytic stereoselective methods development is the need to rapidly determine the enantioselectivity of novel chiral compounds prepared for each single reaction. Such requirement relies almost exclusively on the use chiral separation methods for determining accurate enantiomeric ratios (e.r.) and gauging reaction effectiveness. This challenging feature is compounded by the fact that most of the compounds have never been synthesized before. Consequently, there is a crucial need for the ability to rapidly and accurately determine e.r.s for chiral compounds that result from reaction optimization (e.g., catalyst, chiral ligand, base, solvent, temp., etc.), especially those that contain >1 stereocenter. These data are essential for developing the most efficient and stereoselective transformations that allow synthetic chemists to prepare key biologically relevant compounds in high enantioselectivity (>95:5 e.r.). Supercritical fluid chromatography is a mainstay for such analyses. We previously had a working SFC instrument for chiral separation and e.r. determination. This instrument is 8 years old and is now broken beyond repair due to the large volume of use it received, and its aging components. It should be noted, the SFC in my laboratory was the only working SFC in the department of chemistry for the last two years, and was utilized by other organic groups (e.g., Profs Johnson, Alexanian, Nicewicz, and Aubé) whose instruments were not working or whom do not have a SFC but required its capabilities. At present we are limited to evaluating the enantiomeric ratio by high performance liquid chromatography (HPLC) analysis. Chiral HPLC analysis is time consuming, wasteful, and problematic, particularly for amines (see Scheme 1) and diastereomeric mixtures (i.e., >1 stereocenter). The inability to swiftly analyze material can delay a project by weeks, or months, depending on the functional groups. Chiral SFC is critical to analyzing the chiral boron-containing alcohols and amines generated in this program: We have encountered many examples during our studies of catalytic enantioselective additions of allyl nucleophiles to electrophiles where enantiomer separation and e.r. determination is feasible and more efficient by chiral SFC. For example, as illustrated in Scheme 3 the enantiomers of tertiary Scheme 3. Example of Chiral SFC versus HPLC: alcohol 4 could not be successfully separated by HPLC with many Enantiomeric Ratio Determination Tertiary Alcohol 4  OH different columns and solvent combinations in order to obtain an Me2N N 10 mol% CuOtBu accurate e.r. In contrast, chiral SFC with a super critical CO2 mobile O 12 mol% L1 Me B(pin)  Me CD3OD (1.05 equiv) 4 phase proved successful for achieving clean peak separation. The [(pin)B]2HC 72% yield,  Bu THF, -60 °C, 16 h 91:9 e.r. necessity to procure chromatograms with baseline peak separation · Attempted unsuccessful e.r. analysis of 4 by HPLC: can cost days, if not weeks of time. Now that our SFC has recently broken and is unrepairable, analysis of reaction enantioselectivity takes longer and is often unreliable. Loss of such an important analysis method could not be predicted in advance, but we anticipate · Rapid e.r. analysis of alcohol 4 by SFC instrument the need to maintain such analysis for the duration of this MIRA grant. The requested funds will provide chiral SFC instrumentation that will allow us to reliably and rapidly develop new highly enantioselective reactions for the generation of stereochemically complex, single enantiomer pharmaceuticals important to improving human health. Instrument Usage & Anticipated Future Reoccurring Costs: Five graduates, and one undergraduate researcher working under the support of this MIRA grant, are all involved in stereoselective synthesis projects and require e.r. determination on a regular basis. Based on our 8 years of prior experience with chiral SFC instruments, reoccurring future costs associated with equipment maintenance and upkeep will be able to be sufficiently covered under the current grant funding to ensure instrument longevity of ³8 years.

PI_NAMEs: MEEK, SIMON JOHN (contact)

PROJECT_TERMS: 3-Dimensional;8 year old;Acetone;Administrative Supplement;Aging;Alcohols;Amides;Amines;Amino Acids;base;Biological;Biomedical Research;Boron;carbanion;Carbon;Carbon Dioxide;Catalysis;catalyst;Chemicals;Chemistry;Complex;Consumption;Copper;cost;Coupling;Data;Development;diazene;drug discovery;Effectiveness;enantiomer;Ensure;Equipment;experience;Fluorine;formamide;functional group;Funding;Future;Generations;Goals;Grant;Health;High Pressure Liquid Chromatography;Human;improved;In Situ;instrument;instrumentation;Intercept;interest;Laboratories;Ligands;Longevity;Maintenance;Metals;method development;Methods;Molecular;National Institute of General Medical Sciences;new technology;Nitriles;novel;novel therapeutics;Organic Synthesis;Pharmaceutical Preparations;Pharmacologic Substance;Phase;phosphoramidite;Preparation;Process;programs;Reaction;Reagent;repaired;Research;Research Personnel;scaffold;Scheme;Site;Solvents;Structure;Supercritical Fluid Chromatography;System;Technology;Therapeutic Agents;Time;Transition Elements;undergraduate student;United States National Institutes of Health;Variant;Work

PROJECT_TITLE: NIH Administrative Supplement to purchase an Agilent 1260 Infinity II SFC System


NIH_SPENDING_CATS: Complementary and Integrative Health

ORG_CITY: UNIVERSITY PARK

ORG_NAME: PENNSYLVANIA STATE UNIVERSITY, THE

PHR: Project Narrative Non-heme-iron and 2-oxo-glutarate-dependent enzymes perform an incredible array of oxidative transformations in biosynthetic pathways of medicinal natural products. The parent project (R01 GM141284) seeks to understand which structural factors allow the metallocofactor in these enzymes to perform various reactions. This supplement requests a purchase of a cryogenic system that will allow to perform continuous wave EPR measurements at high frequencies.

PI_NAMEs: SILAKOV, ALEXEY  (contact)

PROGRAM_OFFICER_NAME: SIMONOVIC, MILJAN

PROJECT_TERMS: alpha ketoglutarate;analog;Binding;Carbon;cofactor;Complex;cryogenics;Cyclization;Data;dehydrogenation;Drug Industry;drug synthesis;Enzymes;ferryl iron;Frequencies;geometric structure;Geometry;Glutarates;halogenation;Hydrogen Bonding;Hydroxylation;Individual;innovation;insight;Iron;Measurement;Medicine;member;Methodology;Mixed Function Oxygenases;Natural Product Drug;Natural Products;novel;Outcome;Oxygen;Oxygenases;parent project;Pathway interactions;Phenotype;Positioning Attribute;Process;Reaction;Role;structural determinants;Structure;Synthesis Chemistry;synthetic drug;System;Work

PROJECT_TITLE: The role of geometric structure in avoidance of oxygen rebound to enable aliphatic halogenation and oxacyclization by non-heme Fe(IV)-oxo (ferryl) complexes

STUDY_SECTION_NAME: Special Emphasis Panel[ZRG1(02)-M]


NIH_SPENDING_CATS: Genetics

PHR: NIH Administrative Supplement to Purchase a Lyophilizer Overview This administrative supplement requests funds to purchase a replacement for our 10+ year old Labconco lyophilizer in support of projects funded by NIGMS (5R35GM145227-02; Miles Fabian, PO). Justification for the equipment. This equipment purchase is needed as we have exhausted efforts to repair the current 10+ year-old instrument. The equipment is required to accomplish aspects of every goal in the grant. The lyophilizer is necessary to isolate pure peptides and other water-soluble compounds after purification by removing acetonitrile-water-trifluoroacetic acid mixtures. Without a functioning lyophilizer, none of the projects in this grant (5 R35GM145227-02) can move forward. Key features for the lyophilizer to accomplish this work include acid-resistance and a temperature range down to -84 ° C to be able to remove acetonitrile. At the time of the grant submission, other equipment needs took priority over a lyophilizer, but now a new lyophilizer is our highest priority to make progress on this funded research. Justification for funding. The net cost of the instrument is $ 38,161.00. With encumbrances for personnel, student travel to a conference, and supply needs, less than 20% remains unobligated for the current budget year for this grant.

PI_NAMEs: WATERS, MARCEY L (contact)

PROJECT_TERMS: 10 year old;Acetonitriles;Acids;Acylation;Administrative Supplement;Binding;Biological;Biology;Budgets;Chemicals;chromatin remodeling;cost;Detection;Development;Disease;Engineering;Equipment;equipment acquisition;exhaust;Funding;Goals;Grant;Histones;Human Resources;improved;inhibitor;insight;instrument;Label;Lysine;Methods;Methylation;Molecular;Molecular Probes;National Institute of General Medical Sciences;Nucleosomes;Outcome;Pathway interactions;Peptides;Post-Translational Protein Processing;Proteins;Reader;repaired;Research;Resistance;Role;Site;Students;symposium;Temperature;Time;tool;Travel;Trifluoroacetic Acid;United States National Institutes of Health;Water;Work

STUDY_SECTION: MRAB

STUDY_SECTION_NAME: Maximizing Investigators’ Research Award B Study Section[MRAB]


NIH_SPENDING_CATS: Bioengineering;Cancer;Cancer Genomics;Coronaviruses;Emerging Infectious Diseases;Genetics;Human Genome;Infectious Diseases;Networking and Information Technology R&D (NITRD)

ORG_CITY: NEW YORK

ORG_NAME: NEW YORK UNIVERSITY

PHR: Project Narrative  Using new multiscale biomolecular modeling and simulation methods that can yield mechanistic insights, Schlick's multidisciplinary team will address fundamental challenges in biology in relation to human disease: genome reorganization in cancer processes and RNA co-transcriptional folding in viral mechanisms. A linking of the many relevant biological scales and heterogeneous features of each is essential for understanding the complex biological activities of these macromolecules in the cellular environment and how they aﬀect gene expression, cancer progression, and viral infection. Such an improved understanding of the organization and transition mechanisms associated with the genetic material and its RNA cousin, together with establishment of systematic methods for the study of their heterogeneous conformational landscapes, has direct medical ramiﬁcations by suggesting speciﬁc molecular design and targeted therapies for the treatment of genetic syndromes, degenerative diseases, cancers associated with chromosomal or epigenetic abnormalities, and viral infections like Covid-19.  Speciﬁc Aims  Through this NIGMS Administrative Supplement, the PI seeks to obtain a dedicated 10-GPU (Graphics Processing Units) computing cluster for the Schlick lab to enhance, facilitate, and greatly accelerate the lab's computations and simulations of gene, RNA, and related systems to address fundamental biological challenges in new ways. The parent grant has speciﬁc aims related to elucidating structure/function relationships in genome and RNA systems by novel multiscale techniques. The currently available system at NYU is a large cluster with limited GPU access that is shared by four thousand researchers. While access was easier when the system was ﬁrst installed, large bottlenecks are now associated with thousands of eager users that result in large queue times and cancelled or interrupted jobs. Having the dedicated GPU system will enhance our productivity enormously and allow easy development and testing of new GPU-tailored algorithms for multiscale modeling of nucleic-acid systems of great importance to basic science and human medicine.  Because researcher salary was priority for the PI in the MIRA budget, very modest equipment budgets were requested in both the original MIRA and renewal MIRA proposals. This request represents the ﬁrst major instrumentation request for the Schlick lab for the NIGMS MIRA project.  Introduction to Application  RE: Administrative Supplement for Equipment Purchases for Select NIGMS-Funded Awards for 2R35GM122562-06: NOT-GM-24-021  I am requesting an Administrative Supplement to purchase a 10-GPU (Graphics Processing Unit) computer system from Nvidia to be connected to the Schlick Lab Computer Cluster. This powerful system will provide our researchers direct and rapid access to computations and data storage that are not possible through the large NYU High-Performance Cluster serving thousands of other researchers, all competing for the same queues, especially for GPUs. This 10-GPU system will greatly facilitate, accelerate, and enhance computation and storage for our essential molecular modeling and simulations of genome systems at multiscale resolutions, RNA systems at the all-atom and coarse-grained levels, and related DNA/protein complexes at atomic resolution. The requested system has 10 Nvidia RTX 6000 Ada Graphics Processing Units, 256 AMD CPU cores, 5TB of memory, and 340 TB disk space. The quote from THINKMATE is $249,758.88 for this conﬁguration was solicited.  This powerful GPU system will greatly enhance the productivity of our MIRA research projects and the group facilities. This system was not requested in the original submission because priority was placed on researcher salaries within the limited budget.  The system will be maintained by Mr. Shereef Elmetwaly, the Chemistry Department's Scientiﬁc Computing Manager, whose oﬃce is in our lab space. Mr. Elmetwaly has maintained our computer systems for decades and is well experienced in the job and familiar with all researcher and their needs and many hardware and software systems for molecular simulations. Any supplies and warranty needs not covered in the original system will be covered by the parent grant.

PI_NAMEs: SCHLICK, TAMAR  (contact)

PROJECT_TERMS: 2019-nCoV;Acceleration;Address;Administrative Supplement;Affect;Algorithms;Anti-viral Therapy;Atlases;Award;Basic Science;Biological;Biology;biophysical analysis;biophysical model;Biophysical Process;Biophysics;Budgets;cancer cell;cancer genomics;cancer therapy;Chemicals;Chemistry;Chromatin;Chromatin Fiber;Chromosomes;cluster computing;Collaborations;Communities;Complex;Computational Biology;computer cluster;computer science;Computer Systems;Coronavirus;Coronavirus Infections;COVID-19;Data;Data Storage and Retrieval;Dedications;Degenerative Disorder;design;Development;Diagnosis;Disparate;DNA;Elements;Engineering;Environment;Epigenetic Process;epigenomics;Equipment;equipment acquisition;experience;experimental study;Funding;Gene Activation;Gene Expression;gene repression;Gene Silencing;Genes;Genetic;Genetic Materials;Genetic Transcription;Genome;genome-wide;Grain;Graph;Higher Order Chromatin Structure;HIV;Human;human disease;improved;innovation;insight;instrumentation;Interruption;Lead;Link;macromolecule;Malignant Neoplasms;Mathematics;Medical;Medicine;Memory;Methods;Modeling;models and simulation;Modernization;Molecular;Molecular Conformation;molecular dynamics;molecular modeling;Motion;multi-scale modeling;multidisciplinary;mutant;Mutation;National Institute of General Medical Sciences;novel;Nuclear;Nucleic Acids;Nucleosomes;Occupations;parent grant;Performance;Play;Process;Productivity;protein complex;Proteins;Research Personnel;Research Project Grants;Resolution;Ribosomal Frameshifting;RNA;RNA Conformation;RNA Editing;RNA Virus Infections;Role;scientific computing;simulation;software systems;Structure;Syndrome;System;targeted treatment;Techniques;Technology;Testing;tumor progression;Viral;viral RNA;Virus;Virus Diseases;Wages

PROJECT_TITLE: Bridging Disparate Structural/Functional Scales:  Multiscale Modeling of Genome Organization and if Viral RNA Frameshifting


ADMINISTERING_IC: AA

IC_NAME: NATIONAL INSTITUTE ON ALCOHOL ABUSE AND ALCOHOLISM

NIH_SPENDING_CATS: Alcoholism, Alcohol Use and Health;Chronic Liver Disease and Cirrhosis;Digestive Diseases;Liver Disease;Substance Misuse

ORG_CITY: LOUISVILLE

ORG_NAME: UNIVERSITY OF LOUISVILLE

ORG_STATE: KY

PHR: Project Narrative About 2.5% of the US population is affected by alcohol-associated liver disease (ALD), which represents the 8th most common cause of mortality. Currently there is no FDA approved therapy available for the treatment of ALD. This project will examine phosphodiesterase 4 (PDE4)-mediated pathogenic pathways in ALD. The findings of this project will lead to the development of novel therapies targeting PDE4 for treatment of ALD.

PI_NAMEs: GOBEJISHVILI, LEILA  (contact)

PROGRAM_OFFICER_NAME: JUNG, KATHY

PROJECT_TERMS: ADD-1 protein;Affect;alcohol effect;Alcoholic Hepatitis;Alcoholic Liver Diseases;Alcohols;Animal Model;Apoptosis;Attenuated;Automobile Driving;cell injury;Cell model;Cell Physiology;Cessation of life;Complex;Cyclic AMP;Cyclic AMP-Dependent Protein Kinases;CYP2E1 gene;Cytochrome P450;Data;Development;Disease;Enzymes;Ethanol;Fatty Acids;Fatty Liver;FDA approved;Genes;genetic manipulation;Goals;H19 gene;Heavy Drinking;Hepatic;Hepatocyte;HIF1A gene;Hydrolysis;hypoxia inducible factor 1;Impairment;In Vitro;in vitro Model;in vivo;Inflammatory;inhibitor;Injury;Knockout Mice;lipid biosynthesis;lipid metabolism;Liver;Liver diseases;liver injury;MAPK8 gene;Mediating;member;Metabolic;Mitogen-Activated Protein Kinases;mortality;new therapeutic target;novel;oxidation;Pathogenesis;Pathogenicity;Pathway interactions;Patients;PDE4B;phosphatase-1 kinase;phosphodiesterase IV;phosphoric diester hydrolase;Phosphoric Monoester Hydrolases;Phosphorylation;Play;Population;preclinical study;Process;Regulation;response;Role;Second Messenger Systems;Signal Transduction;Specificity;Toxic effect;transcription factor;Transcriptional Regulation;United States;Untranslated RNA;Up-Regulation;Work

PROJECT_TITLE: Phosphodiesterase 4 mediated pathogenic mechanisms in alcohol associated liver disease

STUDY_SECTION: HBPP

STUDY_SECTION_NAME: Hepatobiliary Pathophysiology Study Section[HBPP]


NIH_SPENDING_CATS: Bioengineering;Biomedical Imaging

ORG_CITY: AMHERST

ORG_NAME: STATE UNIVERSITY OF NEW YORK AT BUFFALO

PHR: Development and Applications of Bioorthogonal Chemistry: Equipment Supplement Narrative This application seeks to combine the powers of genetic code expansion with bioorthogonal chemistry to develop new tools and capabilities for the studies of transmembrane receptor dynamics, intracellular enzyme functions, and antibody-based diagnostics in living systems. This Equipment Supplement requests the acquisition of a Sartorius OCTET-R2 Biolayer Interferometry (BLI) system, which enables us to rigorously characterize covalent binding of protein substrates toward the designed enzyme traps as well as optimize the nanobody- based reaction partners for the HS-based immunoPET radiotracers.

PI_NAMEs: LIN, QING  (contact)


ADMINISTERING_IC: TW

ED_INST_TYPE: SCHOOLS OF PUBLIC HEALTH

FUNDING_MECHANISM: OTHER RESEARCH-RELATED

IC_NAME: FOGARTY INTERNATIONAL CENTER

NIH_SPENDING_CATS: Clinical Research

ORG_CITY: BOSTON

ORG_DEPT: PUBLIC HEALTH & PREV MEDICINE

ORG_NAME: HARVARD UNIVERSITY D/B/A HARVARD SCHOOL OF PUBLIC HEALTH

PHR: Contact PD/PI: Fawzi, Wafaie W Project Narrative The HBNU Fogarty Global Health Training Program, a consortium of Harvard University, Boston University, Northwestern University and the University of New Mexico, proposes to continue to contribute to global health research capacity building and the use of research to improve the health outcomes related to priority health areas in low- and middle-income countries (LMICs) in partnership with 18 sites throughout Africa, Asia, and South America. The Program will recruit US and LMIC trainees to strengthen global health research networks and programs at academic institutions and ultimately help sustain global health research in the US and LMICs. Project Narrative Page 8

PI_NAMEs: FAWZI, WAFAIE W (contact);HAMER, DAVIDSON HOWES;HAWKINS, CLAUDIA A;PERKINS, DOUGLAS JAY

PROGRAM_OFFICER_NAME: HAYES, UNJA

PROJECT_TERMS: Award;Biomedical Research;Boston;Cardiovascular Diseases;career;co-infection;Collaborations;Competence;Creativeness;Diabetes Mellitus;Discipline;Disease;E-learning;Education;Evaluation;experience;Fostering;Future;Future Generations;global health;HIV/AIDS;Individual;Institution;Interdisciplinary Study;International;Investments;low and middle-income countries;Malignant Neoplasms;Maternal and Child Health;medical schools;member;Mental Health;Mentors;Morbidity - disease rate;mortality;New Mexico;next generation;nutrition;Output;Peer Review;Persons;Postdoctoral Fellow;pre-doctoral;Principal Investigator;programs;Public Health Schools;Publications;Qualifying;Readiness;recruit;Research;research and development;Research Design;Research Methodology;Research Project Grants;Research Support;Research Training;Scientist;Site;Students;symposium;tool;Training;Training Programs;Underrepresented Populations;United States National Institutes of Health;Universities

PROJECT_TITLE: Launching Future Leaders in Global Health (LAUNCH) Research Training Program - Supplement for OBSSR

STUDY_SECTION_NAME: Special Emphasis Panel[ZRG1(58)-R]


NIH_SPENDING_CATS: Clinical Research;Genetics;Human Genome;Women's Health Research

ORG_DEPT: OBSTETRICS & GYNECOLOGY

ORG_NAME: WEILL MEDICAL COLL OF CORNELL UNIV

PHR: NARRATIVE Human cells employ several mechanisms to avoid genomic instability at repetitive sequences that can lead to genomic alterations and diseases. My laboratory is interested in unraveling these mechanisms and detect errors during processing of repetitive DNA or RNA sequences that could lead to genomic instability and cell dysfunction.

PI_NAMEs: GERHARDT, JEANNINE  (contact)

PROGRAM_OFFICER_NAME: REDDY, MICHAEL K

PROJECT_TERMS: Adopted;Apoptosis;BRCA1 gene;Cell Death;Cell Physiology;Cell Proliferation;Cells;Chromosome Fragility;Disease;DNA;DNA biosynthesis;DNA Damage;DNA Repair;DNA Repair Pathway;DNA replication fork;DNA Sequence;DNA Sequence Alteration;Functional disorder;Generations;Genetic Transcription;Genome;Genomic Instability;Genomics;Goals;Human;interest;Knowledge;Laboratories;Messenger RNA;Molecular;Mutation;Myopathy;Nervous System Disorder;Nuclear;Oocytes;Organism;Outcome Study;Ovarian Diseases;Pathway interactions;polypeptide;prevent;Process;programs;protein expression;Proteins;repaired;Repetitive Sequence;Research;RNA;RNA Sequences;Structure;Translation Initiation;Translations;Travel

PROJECT_TITLE: Repetitive sequences causing genomic instability and cell dysfunction


ADMINISTERING_IC: TR

FUNDING_MECHANISM: RESEARCH CENTERS

IC_NAME: NATIONAL CENTER FOR ADVANCING TRANSLATIONAL SCIENCES

NIH_SPENDING_CATS: Clinical Research;Clinical Trials and Supportive Activities;Precision Medicine

ORG_CITY: GAINESVILLE

ORG_DEPT: NEUROSURGERY

ORG_NAME: UNIVERSITY OF FLORIDA

ORG_STATE: FL

PHR: Overall Narrative NARRATIVE The University of Florida (UF) and the Florida State University (FSU) CTSA hub will work within the demographically and geographically diverse state of Florida to improve human health by accelerating the translation of scientific discoveries and the implementation of evidence-based best practices for the diagnosis, treatment, prevention and cure of human diseases across the lifespan. The 2019-2024 period represents the next phase of evolution from creating a clinical and translational science infrastructure to enhancing the local, state and national impact of CTSI-led science with a special emphasis on underserved and unique patient populations.

PI_NAMEs: MITCHELL, DUANE A. (contact)

PROGRAM_OFFICER_NAME: DIBELLO, ANTHONY THOMAS

PROJECT_TERMS: Acceleration;Aging;career development;Catchment Area;Clinical;Clinical Informatics;Clinical Research;Clinical Sciences;Clinical Trials Network;Collaborations;college;community engagement;Computer software;Country;data integration;Dedications;Diagnosis;disabled;Environment;evidence base;Evolution;Faculty;Florida;genomic data;Genomic medicine;Geography;Goals;Health;Health Priorities;Health system;Healthcare;Historically Black Colleges and Universities;Human;human disease;implementation science;improved;Informatics;Infrastructure;innovation;Institution;Learning;life span;Medicaid;metabolomics;Mission;Modeling;Participant;Pathway interactions;patient population;Patient-Focused Outcomes;personalized medicine;Phase;Policies;precision medicine;Prevention;programs;recruit;Research;Science;Site;success;Surgeon;training opportunity;Translating;Translational Research;Translations;Trust;Underrepresented Minority;Universities;Vision;Work

PROJECT_TITLE: Together: Transforming and Translating Discovery to Improve Health


NIH_SPENDING_CATS: Bioengineering;Nanotechnology

ORG_NAME: BOSTON UNIVERSITY (CHARLES RIVER CAMPUS)

PHR: UV Plasmon-Enhanced Chiroptical Spectroscopy of Membrane-Binding Proteins Relevance to Public Health Membrane binding proteins have diverse important biological functions ranging from controlling signaling to regulating membrane fusion but are difficult to characterize with existing biophysical tools. This project develops electromagnetically enhanced chiroptical spectroscopies that can provide detailed structural information about membrane-binding proteins under physiological conditions and will, thus, lead to a greatly improved understanding of these important proteins with important disease diagnostic implications.

PI_NAMEs: REINHARD, BJOERN MARKUS (contact)

PROGRAM_OFFICER_NAME: YEH, ALVIN TIEN-WEI

PROJECT_TERMS: aqueous;Binding;Binding Proteins;Binding Sites;Biological;Biological Process;biophysical properties;biophysical tools;Buffers;Circular Dichroism;Circular Dichroism Spectroscopy;Crystallization;design;disease diagnostic;Electromagnetics;Electronics;Hot Spot;improved;insight;instrument;interest;Lead;Lipids;Magnetism;Membrane;Membrane Fusion;Membrane Lipids;Membrane Proteins;Methods;Molecular;nano;nanoengineering;Nanostructures;Optics;Pharmacologic Substance;Phase;Physiological;plasmonics;Preparation;Property;protein structure;Proteins;prototype;Public Health;Sampling;Signal Transduction;Spectrum Analysis;Structure;Surface;theories;tool;ultraviolet

STUDY_SECTION_NAME: Special Emphasis Panel[ZRG1(03)-M]


NIH_SPENDING_CATS: Pediatric

ORG_CITY: MILWAUKEE

ORG_NAME: MEDICAL COLLEGE OF WISCONSIN

ORG_STATE: WI

PHR: Document not required

PI_NAMEs: BAI, XIAOWEN  (contact)

PROGRAM_OFFICER_NAME: JUSTINOVA, ZUZANA

PROJECT_TITLE: Mitochondria and anesthetic-induced developmental neurotoxicity

STUDY_SECTION: MRAC

STUDY_SECTION_NAME: Maximizing Investigators' Research Award C Study Section[MRAC]


NIH_SPENDING_CATS: Aging;Breast Cancer;Cancer;Genetics;Stem Cell Research;Stem Cell Research - Nonembryonic - Non-Human;Women's Health Research

PHR: Project Narrative: The N-terminal methyltransferase NRMT1 plays important roles in cell growth and survival. By combining study of its regulation, physiological substrates, and loss of function phenotypes, we aim to pursue a global approach to developing NRMT1 as a potential therapeutic for human cancers and age-related disorders.

PI_NAMEs: SCHANER-TOOLEY, CHRISTINE E (contact)

PROGRAM_OFFICER_NAME: BARSKI, OLEG

PROJECT_TERMS: age-related disease;Aging;amino group;Bacteria;Binding Proteins;Biochemistry;Biological;Breast Cancer Cell;Cell Compartmentation;cell growth;Cell Survival;Code;Consensus Sequence;DNA;Enzymes;Goals;Growth;Histones;Human;Knock-out;Kyphosis deformity of spine;loss of function;Malignant Neoplasms;Mammals;Methylation;Methyltransferase;migration;Mus;Myosin Light Chains;N-terminal;Pattern;Phenotype;Physiological;Play;Post-Translational Protein Processing;Premature aging syndrome;Process;Proliferating;Proteins;Regulation;Role;skin fibrosis;stem cell fate;Therapeutic;tumorigenesis

PROJECT_TITLE: Expanding the biological roles of N-terminal methylation


NIH_SPENDING_CATS: Cancer;Genetics

ORG_NAME: UNIVERSITY OF MICHIGAN AT ANN ARBOR

PHR: Project Narrative: Genomic integrity is challenged by spontaneous DNA damage that results from endogenous and exogenous sources. This proposal focuses on elucidating the molecular mechanisms by which cells detect and repair the most common forms of DNA damage, especially single strand breaks and damaged bases. This information is critical to understanding the impact of genetic deficiencies, exposure to carcinogens, and to improving cancer therapy. We employ a wide variety of genetic, cellular, biochemical and biophysical approaches to determine molecular mechanisms which are predictive of cellular behavior in both normal and disease states. Purchase of a TwoMP mass photometer will provide a needed tool for molecular characterization of macromolecular complexes at physiological concentrations that has not been previously possible.

PI_NAMEs: O'BRIEN, PATRICK J (contact)

PROGRAM_OFFICER_NAME: MACARTHUR, LINDA

PROJECT_TERMS: Age;base;Base Excision Repairs;Biochemical;Biological;biophysical techniques;Biophysics;cancer therapy;Carcinogens;cell behavior;Cell Death;Cells;Clinical;Disease;DNA Damage;DNA Repair;DNA Repair Enzymes;DNA Repair Pathway;Enzymes;Exposure to;Failure;Genetic;genome integrity;Genotoxic Stress;Genotype;Goals;Human;improved;interdisciplinary approach;Kinetics;Macromolecular Complexes;Malignant Neoplasms;Modeling;Molecular;Mutagenesis;Mutation;Phenotype;Physiological;repaired;Risk;Site;Somatic Mutation;Source;Structure;System;Time;tool;Training;Translating;Work

PROJECT_TITLE: Mechanisms of Base Excision DNA Repair