NIH_SPENDING_CATS: Bioengineering;Congenital Structural Anomalies;Genetics;Pediatric;Wound Healing and Care

ORG_CITY: DURHAM

ORG_NAME: DUKE UNIVERSITY

PHR: Morphogenesis, the biological process that causes cells or tissues to attain their characteristic shape, is highly conserved throughout phylogeny, is fundamental to development and wound healing and can best be investigated through precise, high-resolution documentation in both time and space. We use powerful genetic and biophysical approaches to investigate cell sheet morphogenesis in the simple system, Drosophila melanogaster and request here upgrades to our > 20 year old microscope instrumentation that is out of date and badly underperforming. Our studies are designed to identify new genes that regulate and drive cell sheet movements and investigate the molecular basis for how these genes c0ntribute to morphogenesis – we provide important insights into the basic biological mechanisms that go awry in human pathologies such as cardia bifida, spina bifida, cleft palate and wound-induced, epithelial lesions.

PI_NAMEs: KIEHART, DANIEL PETER (contact)

PROGRAM_OFFICER_NAME: WELLS, MELISSA LYNN

PROJECT_TERMS: 20 year old;Administrative Supplement;Biological;Biological Process;biophysical techniques;Biophysics;Cells;Characteristics;Cleft Palate;Computer software;Computers;Data;design;Development;digital;Documentation;Dorsal;Drosophila melanogaster;Embryo;Embryonic Development;Epithelium;Equipment;experimental study;Frequencies;Funding;Genes;Genetic;genetic approach;Genetic Screening;Head;Human Pathology;Image;imaging capabilities;insight;Institution;instrumentation;Investigation;Lasers;Lesion;Microscope;Microsurgery;Modernization;Molecular;Morphogenesis;Movement;Mutation;National Institute of General Medical Sciences;Noise;Operative Surgical Procedures;Parents;Phylogeny;Resolution;Resources;Running;Security;sensor;Shapes;Specimen;Speed;Spinal Dysraphism;stomach cardia;System;Technology;Time;Tissues;wound;wound healing

PROJECT_TITLE: Morphogenesis: Biophysics and Genetics of Dorsal Closure


ADMINISTERING_IC: HL

ARRA_FUNDED: N

ED_INST_TYPE: SCHOOLS OF MEDICINE

FUNDING_MECHANISM: NON-SBIR/STTR RPGS

IC_NAME: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE

NIH_SPENDING_CATS: Bioengineering;Biotechnology;Cardiovascular;Heart Disease;Regenerative Medicine;Stem Cell Research;Stem Cell Research - Induced Pluripotent Stem Cell;Stem Cell Research - Induced Pluripotent Stem Cell - Human

ORG_CITY: MINNEAPOLIS

ORG_COUNTRY: UNITED STATES

ORG_DEPT: BIOMEDICAL ENGINEERING

ORG_FIPS: US

ORG_NAME: UNIVERSITY OF MINNESOTA

ORG_STATE: MN

PHR: Project Narrative This work will bolster our understanding of epicardial-myocardial signaling and the role of the extracellular matrix in modulating cell behavior in the developing heart. The clinical relevance of this work lies in the application of epicardial cells to enhance the pump function of a perfusable, chambered, human cardiac tissue model that will be ideal to study the impact of cardiac disease and therapeutics on pressure-volume dynamics, stroke work, and ejection fraction. In the future, this living, human muscle pump generated in a dish might be used to restore cardiac function in patients with heart disease.

PI_NAMEs: OGLE, BRENDA M (contact)

PROGRAM_OFFICER_NAME: THAKAR, RAHUL GAUTAM

PROJECT_TERMS: 3-Dimensional;3D Print;Automobile Driving;Behavior;Biochemical;Bioreactors;Cardiac;Cardiac Myocytes;cardiac tissue engineering;Cardiovascular Diseases;Cardiovascular system;Catheters;cell behavior;Cell Density;Cell secretion;cell type;Cells;Clinical;clinically relevant;Complex;Coronary;Coupling;Cues;Deposition;design;Development;Developmental Process;Devices;Disease;disease model;drug testing;Drug usage;EFRAC;Electric Stimulation;Embryo;Environment;Epicardium;epithelial to mesenchymal transition;extracellular;Extracellular Matrix;Extracellular Matrix Proteins;Fibroblast Growth Factor;Fibroblasts;Future;Generations;Genes;Growth Factor;Heart;heart cell;Heart Diseases;heart function;Human;human induced pluripotent stem cells;human model;human tissue;implantation;improved;In Vitro;in vivo;induced pluripotent stem cell derived cardiomyocytes;insight;Insulin-Like Growth Factor II;Integrins;interest;Interleukin-2;Knowledge;Link;Mass Spectrum Analysis;Mechanical Stimulation;Medicine;migration;millimeter;Modeling;Morphogenesis;Morphology;Mus;Muscle;Myocardial;Myocardium;next generation;novel;Organoids;Output;Paracrine Communication;Patients;Phenotype;Play;Pluripotent Stem Cells;pressure;Pressure Transducers;Process;Proliferating;Protein Secretion;Proteins;Proteomics;Pump;Regulation;Role;Sarcomeres;Signal Transduction;Smooth Muscle Myocytes;stem cells;Stroke;Structure;Supporting Cell;Testing;Therapeutic;Thick;Time;Tissue Engineering;Tissue Model;Tissues;Vascular Smooth Muscle;Ventricular;Work

PROJECT_TITLE: Epicardial regulation of cardiomyocyte function via modulation of extracellular signals: toward a model of human muscle pump function

STUDY_SECTION: MPPA

STUDY_SECTION_NAME: Integrative Myocardial Physiology/Pathophysiology A Study Section [MPPA]


ED_INST_TYPE: HOSPITALS

FUNDING_MECHANISM: OTHER RESEARCH-RELATED

NIH_SPENDING_CATS: Biotechnology;Clinical Research;Genetics;Hematology;Human Genome;Rare Diseases

ORG_CITY: HOUSTON

ORG_DEPT: INTERNAL MEDICINE/MEDICINE

ORG_NAME: UNIVERSITY OF TX MD ANDERSON CAN CTR

ORG_STATE: TX

PHR: PROJECT NARRATIVE High-risk molecular mutations in the epigenetic regulators ASXL1 and EZH2 co-occur frequently with JAK2V617F activating mutations in myeloproliferative neoplasms (MPNs) and portend risk of fibrotic progression, shortened duration of response to targeted JAK inhibitor therapy, and increased rate of leukemic transformation. We hypothesize ASXL1/EZH2 mutations cooperate with JAK2V617F to enhance clonal fitness/outgrowth of mutant hematopoietic stem cells and pro-fibrotic inflammatory signaling pathways to impart resistance to JAK inhibitors and promote fibrotic alterations in the bone marrow niche. Our work aims to utilize an optimized inducible and reversible Jak2V617F mutant mouse model, a large cohort of banked MPN patient samples, and an array of in vivo and genomic/epigenomic techniques to understand how epigenetic alterations contribute to clonal/fibrosis evolution in MPNs with the goal of translating our findings into novel therapeutic avenues for MPN patients with high-risk epigenetic alterations.

PI_NAMEs: DUNBAR, ANDREW JEFFREY (contact)

PROGRAM_OFFICER_NAME: GILLASPY, ALLISON

PROJECT_TERMS: Academic Medical Centers;Alleles;Bioinformatics;Biological Assay;Bone Marrow;burden of illness;career development;Cell surface;cell type;Cells;Chromatin;Chromatin Remodeling Factor;Chronic;Clinical;clinical translation;Clonal Evolution;cohort;Complement;conditional knockout;Coupled;cytokine;Dedications;Dependence;Development;Development Plans;Disease;Disease Progression;DNA sequencing;Engraftment;Epigenetic Process;epigenetic regulation;epigenomics;Evolution;EZH2 gene;Fibrosis;fitness;Frequencies;Funding;gain of function mutation;Gene Expression;Genetic Transcription;Genomics;Genotype;Germany;Glean;Goals;Hematologic Neoplasms;Hematopoietic;Hematopoietic stem cells;high risk;Human;Immunophenotyping;improved;in vivo;Individual;Inflammatory;inhibitor;inhibitor therapy;insight;JAK2 gene;Knock-in;Knock-out;Knockout Mice;Laboratories;leukemic transformation;Longitudinal Studies;loss of function;loss of function mutation;Maintenance;Maps;Mediating;Memorial Sloan-Kettering Cancer Center;Mentors;mesenchymal stromal cell;Methods;Modeling;Molecular;mouse model;Mus;mutant;mutant mouse model;Mutation;Myelofibrosis;Myelogenous;Myeloproliferative disease;novel;novel therapeutics;Oncogenic;Output;Paper;Pathogenesis;Pathway interactions;patient subsets;Patients;Polycomb;Polycythemia Vera;Primary Myelofibrosis;Process;Production;Prognosis;programs;Proliferating;protein profiling;Publishing;recombinase;Reporter;Research;Research Personnel;Resistance;response;Risk;Sampling;Secondary to;Signal Pathway;Signal Transduction;Specimen;Technical Expertise;Techniques;Therapeutic;Therapeutic Studies;therapy resistant;Time;Training;Translating;Transplant Recipients;treatment response;University Hospitals;Work

PROJECT_TITLE: Investigating High-Risk Epigenetic Modifying Alterations on JAK2VF Dependency and Fibrotic Progression in Myeloproliferative Neoplasms (MPNs)

STUDY_SECTION: MCBS

STUDY_SECTION_NAME: NHLBI Mentored Clinical and Basic Science Study Section[MCBS(MA)]


ADMINISTERING_IC: GM

IC_NAME: NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES

ORG_CITY: CORAL GABLES

ORG_DEPT: NEUROLOGY

ORG_NAME: UNIVERSITY OF MIAMI SCHOOL OF MEDICINE

ORG_STATE: FL

PHR: Project Narrative The goal of the original MIRA award R35GM118141 is to develop a program aiming to understand the biogenesis of mitochondrial membranes. Disorders arising from impaired mitochondrial OXPHOS function or mitochondrial gene expression, including protein synthesis, result in a variety of pathologies including encephalomyopathies and cardiomyopathies. Identifying and characterizing factors required for OXPHOS enzyme assembly, mitoribosome biogenesis and translation, and the pathways in which they act is a prerequisite towards understanding the molecular basis of these disorders. This proposal is for the purchase of the Amersham ImageQuant 800 biomolecular imager. This system is ideal for chemiluminescence, fluorescence, and colorimetric imaging of a wide variety of samples, including gels, membrane blots, multiwell plates, and petri dishes.

PI_NAMEs: BARRIENTOS, ANTONI  (contact)

PROGRAM_OFFICER_NAME: ANSONG, CHARLES KWAKU

PROJECT_TERMS: Award;Biochemistry;Biogenesis;Biological Sciences;Cardiomyopathies;Charge;Computer software;Coupled;Detection;Devices;Disease;Doctor of Philosophy;Enzymes;Fluorescence;Funding;Gel;Gene Expression;Generations;Goals;Health;Image;imager;Impairment;improved;instrument;Intuition;medical schools;Membrane;Mitochondria;mitochondrial membrane;Molecular;Molecular Biology;National Institute of General Medical Sciences;Neurology;Noise;Optics;Pathology;Pathway interactions;professor;programs;Protein Biosynthesis;remote location;Research Personnel;Sampling;Signal Transduction;System;tool;Translations;Universities;X-Ray Film

PROJECT_TITLE: Mitochondrial Biogenesis in Health and Disease


ED_INST_TYPE: BIOMED ENGR/COL ENGR/ENGR STA

NIH_SPENDING_CATS: Bioengineering;Chronic Pain;Natural Products;Pain Research

ORG_CITY: TUSCALOOSA

ORG_DEPT: ENGINEERING (ALL TYPES)

ORG_NAME: UNIVERSITY OF ALABAMA IN TUSCALOOSA

ORG_STATE: AL

PHR: PROJECT NARRATIVE We propose to develop a cold triggered local anesthesia that allows patients to adjust the degree of pain relief according to their changing needs and conditions. With a single perineural injection or site infiltration, the patient can determine the onset of anesthesia, can trigger anesthesia multiple times to mimic the frequent administration of conventional local anesthetics, and can trigger anesthesia for the duration of post-operative pain state.

PI_NAMEs: ZHAO, CHAO  (contact)

PROGRAM_OFFICER_NAME: JUSTINOVA, ZUZANA

PROJECT_TERMS: Anatomy;Anesthesia procedures;aqueous;biomaterial compatibility;Body Temperature;Bupivacaine;capsule;Cell Survival;Characteristics;chronic pain;Clinical;clinical application;Cold Therapy;Collection;cost;cytotoxicity;design;Dose;drug action;Drug Carriers;Drug Kinetics;efficacy evaluation;Encapsulated;Equipment;Event;Extravasation;Feasibility Studies;Formulation;Frostbite;Goals;Hour;Hydrogels;improved;In Vitro;in vivo;in vivo evaluation;in vivo imaging system;Infiltration;Inflammation;Injectable;Injections;Injury;interest;Libraries;Light;Liposomes;Local anesthesia;Local Anesthetics;Long-Term Effects;Medical;Methods;Microscopic;Microspheres;Modeling;Molecular Weight;Morphology;natural hypothermia;operation;Organ;Pain management;pain relief;Patients;perineural;Pharmaceutical Preparations;Plasma;Pluronics;poly(lactide);polyglycerol;Polymers;Porosity;Postoperative Pain;Postoperative Period;Preparation;prevent;Procedures;Property;Propylene Glycols;Rattus;Reaction;Research;response;Rupture;Safety;sciatic nerve;screening;self assembly;Site;Stimulus;Structure;Surgical incisions;Temperature;Tetrodotoxin;Time;Tissues;Toxic effect;ultrasound;Water;wound

PROJECT_TITLE: Equipment Supplement - Cold triggered Local Anesthesia for Pain Management

STUDY_SECTION: GDD

STUDY_SECTION_NAME: Gene and Drug Delivery Systems Study Section[GDD]


ORG_CITY: CHICAGO

ORG_DEPT: BIOLOGY

ORG_NAME: UNIVERSITY OF CHICAGO

ORG_STATE: IL

PHR: Not applicable

PI_NAMEs: SCHMIDT-OTT, URS C (contact)

PROJECT_TERMS: Chironomus thummi;fly;Genetic Variation

PROJECT_TITLE: Genetic variation and function of body axis determinants in midges and other flies


ED_INST_TYPE: SCHOOLS OF ARTS AND SCIENCES

ORG_CITY: NORMAN

ORG_DEPT: CHEMISTRY

ORG_NAME: UNIVERSITY OF OKLAHOMA

ORG_STATE: OK

PHR: Project Narrative of the Funded Parent Award (1R01GM138800-01A1): This proposal seeks to develop a facile method of synthesizing polyprenyl-diphosphates, precursors in the synthesis of thousands of valuable isoprenoids. The utility of the platform will be demonstrated for the synthesis of cannabinoid and squalene analogs for their potential therapeutic applications.

PI_NAMEs: SINGH, SHANTERI  (contact)

PROGRAM_OFFICER_NAME: BOND, MICHELLE RUEFFER

PROJECT_TERMS: Acids;Active Sites;Address;Alcohols;analog;analytical method;Cannabinoids;catalyst;Cells;chemical synthesis;Collaborations;combinatorial;Coupled;Coupling;Diphosphates;Engineering;Environment;Enzymes;Escherichia coli;Farnesol;farnesyl pyrophosphate;Funding;Generations;Geranyltranstransferase;Goals;High Pressure Liquid Chromatography;high throughput screening;Human;In Situ;In Vitro;in vivo;Industry;inorganic phosphate;isopentenyl pyrophosphate;Isoprene;isoprenoid;Kinetics;Knowledge;Libraries;Medicine;Metabolic;metabolic engineering;Metabolic Pathway;Methionine;methionine adenosyltransferase;Methodology;Methods;Methyltransferase;Minnesota;Modernization;Monitor;Mutagenesis;Natural Products;Natural Selections;Natural Source;Nature;non-Native;North Carolina;novel;parent project;Pathway interactions;Phosphorylation;Phosphotransferases;prenyl;Process;Production;Reaction;S-Adenosylhomocysteine;S-Adenosylmethionine;Squalene;Squalene Synthetase;Structure;Structure-Activity Relationship;synthetic biology;System;Technology;Therapeutic;Universities;Variant;Work

PROJECT_TITLE: Acquisition of a Preparative High-Performance Liquid Chromatography (HPLC) System


ADMINISTERING_IC: OD

FUNDING_MECHANISM: OTHERS

IC_NAME: OFFICE OF THE DIRECTOR, NATIONAL INSTITUTES OF HEALTH

NIH_SPENDING_CATS: Acquired Cognitive Impairment;Alzheimer's Disease Related Dementias (ADRD);Alzheimer's Disease including Alzheimer's Disease Related Dementias (AD/ADRD);Bioengineering;Biomedical Imaging;Brain Disorders;Cardiovascular;Cerebrovascular;Clinical Research;Dementia;Eye Disease and Disorders of Vision;Machine Learning and Artificial Intelligence;Neurodegenerative;Neurosciences;Vascular Contributions to Cognitive Impairment and Dementia (VCID)

PI_NAMEs: LIANG, LIANG ;WANG, JIANHUA  (contact);ZHANG, YUHUA LIANG

PROGRAM_OFFICER_NAME: MADDEN, EBONY B

PROJECT_TERMS: abeta deposition;adaptive optics;Affect;Age;Aging;Anatomy;Angiography;Area;arterial spin labeling;Arteries;arteriole;Arterioloscleroses;Attention;automated analysis;Biological Markers;biomechanical model;Blood;Blood capillaries;Blood Circulation;Blood flow;Blood Flow Velocity;blood vessel visualization;Blood Vessels;Brain;Central Nervous System;Central Nervous System Diseases;Cerebral Amyloid Angiopathy;Cerebral hemisphere hemorrhage;cerebral microbleeds;Cerebral small vessel disease;cerebrovascular;Cerebrum;Characteristics;Clinic;cost;density;design;Development;Devices;Eye;Geometry;Health;hemodynamics;Hypertension;Image;imaging detection;Imaging Device;Imaging Techniques;Imaging technology;innovation;innovative technologies;insight;instrument;interdisciplinary approach;Lacunar Infarctions;Light;Machine Learning;machine learning model;macula;Magnetic Resonance Imaging;Measurement;Measures;Medicine;Methods;Microcirculation;Monitor;MRI Scans;neuroimaging;novel;novel imaging technique;novel marker;Ophthalmoscopes;Optical Coherence Tomography;Oxygen;Pathologic;Pathology;Patients;Physiological;potential biomarker;Prevalence;Pulsatile Flow;Reproducibility;Research;Retina;retina blood vessel structure;retina circulation;Sensitivity and Specificity;sex;Speed;Stress;Structure;Study models;synergism;System;Systemic disease;Technology;technology development;Testing;Tissues;Translating;Validation;Variant;vascular cognitive impairment and dementia;vascular risk factor;venule;White Matter Hyperintensity

PROJECT_TITLE: Novel retinal higher-order capillary hemodynamics imaging for detecting cerebral small vessel disease

STUDY_SECTION: CFOT

STUDY_SECTION_NAME: Common Fund Other Transactions Programs[CFOT (03)]


NIH_SPENDING_CATS: Acquired Cognitive Impairment;Aging;Alzheimer's Disease;Alzheimer's Disease including Alzheimer's Disease Related Dementias (AD/ADRD);Bioengineering;Biomedical Imaging;Brain Disorders;Clinical Research;Dementia;Eye Disease and Disorders of Vision;Machine Learning and Artificial Intelligence;Natural Products;Neurodegenerative;Neurosciences;Parkinson's Disease;Prevention

ORG_CITY: NEW YORK

ORG_DEPT: OPHTHALMOLOGY

ORG_NAME: NEW YORK UNIVERSITY SCHOOL OF MEDICINE

ORG_STATE: NY

PI_NAMEs: BALCER, LAURA ;SRINIVASAN, VIVEK JAY (contact)

PROJECT_TERMS: abeta deposition;Accounting;Affect;Age;Alzheimer's Disease;Alzheimer's disease patient;Amacrine Cells;Amyloid;Amyloid beta-Protein;Architecture;Biological Markers;Blood;Blood Vessels;Brain;Carotenoids;cell type;Cells;Central Nervous System;cerebral atrophy;Cerebrospinal Fluid;Circadian Dysregulation;Cognition;Cognitive;comparison control;Contrast Sensitivity;cost;density;Deposition;Diagnosis;Disease;Disease Progression;Dopamine;Early Diagnosis;Eye;Functional disorder;functional loss;ganglion cell;Ganglion Cell Layer;Goals;Histopathology;Human;Image;imaging approach;Imaging technology;Impairment;improved;in vivo;in vivo fluorescence imaging;Inflammation;Inner Nuclear Layer;Inner Plexiform Layer;insight;instrument;Lutein;Machine Learning;macula;Measures;melanopsin;meter;mild cognitive impairment;Monitor;mouse model;Mus;Nerve Degeneration;Nerve Fibers;Neurodegenerative Disorders;Neurologic;next generation;novel;novel strategies;Optical Coherence Tomography;Parkinson Disease;Pathogenesis;Pathology;Pathway interactions;Patients;Performance;Persons;Pharmaceutical Preparations;Pigments;Population;potential biomarker;Preclinical Testing;Productivity;Proteins;Quality of life;Reporting;Research;Resolution;Retina;Retinal Ganglion Cells;retinal imaging;Senile Plaques;specific biomarkers;Specificity;standard measure;Structure;symptom treatment;Synapses;System;tau-1;Technology;Testing;Thinness;tool;Visible Radiation;Vision;Visual;Visual Pathways;Visual System;Visualization;zeaxanthin;β-amyloid burden

PROJECT_TITLE: Achieving specificity in imaging neurodegeneration with visible light Optical Coherence Tomography


NIH_SPENDING_CATS: Neurosciences

ORG_CITY: EVANSTON

ORG_NAME: ENDEAVOR HEALTH CLINICAL OPERATIONS

PHR: We are seeking supplemental funding to purchase an advanced electrophysiological recording system to study the effects of general anesthesia on neuronal activity in the brain.

PI_NAMEs: AKSENOV, DANIIL  (contact)

PROJECT_TERMS: Anesthesia procedures;Animals;Brain;brain tissue;Cerebral cortex;Chronic;Data;design;Development;Electrophysiology (science);Ensure;experimental study;Funding;General Anesthesia;Implanted Electrodes;improved;Learning;Monitor;Neonatal;Neurons;Oxygen;Regulation;Signal Transduction;System;Time;Update;Work

PROJECT_TITLE: Developmental disruption of brain tissue oxygen regulation and deficiency of learning after neonatal anesthesia exposure

STUDY_SECTION: PMDA

STUDY_SECTION_NAME: Pathophysiological Basis of Mental Disorders and Addictions Study Section[PMDA]


NIH_SPENDING_CATS: Acute Respiratory Distress Syndrome;Clinical Research;Genetics;Hematology;Human Genome;Infectious Diseases;Lung;Pediatric;Perinatal Period - Conditions Originating in Perinatal Period;Preterm, Low Birth Weight and Health of the Newborn;Rare Diseases;Sepsis

ORG_CITY: PROVIDENCE

ORG_NAME: RHODE ISLAND HOSPITAL

ORG_STATE: RI

PHR: Project Narrative Despite the use of state-of-the-art supportive care and a variety of novel anti-inflammatory agents in critically ill/injured patients, multiple organ failure continues to be a major cause of morbidity/mortality in the surgical intensive care unit. This study is designed to determine if a family of novel checkpoint proteins, expressed on immune and non-immune cells, can be used to block the development of organ injury in the critically ill patient.

PI_NAMEs: AYALA, ALFRED  (contact)

PROGRAM_OFFICER_NAME: ZHAO, XIAOLI

PROJECT_TERMS: Accounting;Acute Lung Injury;Adoptive Transfer;Age;Animals;Anti-Inflammatory Agents;Apoptosis;Area;ATAC-seq;Automobile Driving;bisulfite;Cause of Death;cecal ligation puncture;Cell surface;Cells;Cessation of life;Clinical Research;comparative;Consensus;Cre-LoxP;Critical Illness;Defect;design;Developed Countries;Development;Endothelium;Epigenetic Process;epigenomics;Epithelial Cells;Etiology;Event;Family;Frustration;Genetic;Hemorrhagic Shock;Hospitals;Immune;immune function;Immune system;Immune System Diseases;Immunoglobulin Domain;Immunologic Memory;Incidence;inhibitor;injured;Injury;Intensive Care Units;Intestines;kidney dysfunction;Leukocytes;Ligands;Liver;Lung;Lymphoid Cell;member;microbiota;Modeling;Molecular;Morbidity - disease rate;mortality;mouse model;Multiple Organ Failure;Myelogenous;neonatal sepsis;neonate;novel;organ growth;organ injury;Outcome;patient population;Patients;Population;Predisposition;Prognostic Marker;programs;protein expression;Protein Family;Proteins;pyrosequencing;receptor;Reporting;response;Role;SARS-CoV-2 infection;Sepsis;septic;Shock;single-cell RNA sequencing;Supportive care;Suppressor-Effector T-Lymphocytes;Surgical Intensive Care;Survivors;T-Cell Activation;T-Lymphocyte;therapeutic target;transcriptome sequencing;transcriptomics

PROJECT_TITLE: Mechanism of Immune Dysfunction and Morbid Outcomes in Response to Shock/Sepsis


NIH_SPENDING_CATS: Acquired Cognitive Impairment;Basic Behavioral and Social Science;Behavioral and Social Science;Brain Disorders;Mental Health;Neurosciences;Women's Health Research

ORG_CITY: BOSTON

ORG_NAME: MASSACHUSETTS GENERAL HOSPITAL

ORG_STATE: MA

PHR: PROJECT NARRATIVE During recovery of consciousness after general anesthesia, common clinical problems occur such as delirium and delayed awakening. There are no available treatments for these problems because the mechanisms underlying recovery of consciousness after general anesthesia are unknown. The goals of this MIRA proposal are to understand how cognition recovers after general anesthesia in rodents, and to test whether recovery of cognition may be accelerated by activating specific neural circuits involved in memory, attention, and arousal.

PI_NAMEs: SOLT, KEN  (contact)

PROJECT_TERMS: Absence of pain sensation;Acceleration;Administrative Supplement;Amnesia;Anesthesia procedures;Anesthetics;Arousal;Attention;Brain;Cell Nucleus;Clinical;Cognition;Cognitive;cognitive recovery;cognitive testing;common treatment;Conscious;Delirium;Estrous Cycle;Female;General Anesthesia;General anesthetic drugs;genetic manipulation;Goals;Grant;insight;Knowledge;Medicine;Memory;neural circuit;novel strategies;Operative Surgical Procedures;programs;Rattus;Recovery;Research;Rodent;Sex Differences;System;Testing;touchscreen;Unconscious State

PROJECT_TITLE: Administrative Supplement: Touchscreen Cognitive Testing System for Studies of Anesthesia and Consciousness


NIH_SPENDING_CATS: Bioengineering;Biomedical Imaging;Cardiovascular;Clinical Research;Eye Disease and Disorders of Vision;Machine Learning and Artificial Intelligence;Networking and Information Technology R&D (NITRD);Neurosciences

ORG_NAME: NORTHWESTERN UNIVERSITY AT CHICAGO

PI_NAMEs: BURNS, STEPHEN A;FAWZI, AMANI A (contact)

PROJECT_TERMS: Acceleration;adaptive optics;Advisory Committees;Algorithms;Alzheimer's Disease;Angiography;Area;Arterial Disorder;arteriole;artificial intelligence algorithm;autosome;Back;Biological Markers;biomarker validation;Blood capillaries;Blood Cells;Blood flow;Blood Vessels;Burn injury;cell motility;Cells;Cerebrum;Chronic Kidney Failure;Clinical;clinical care;clinical decision-making;clinical research site;Color;Computer software;Darkness;Data;Data Set;deep learning;denoising;density;design;Detection;Devices;diabetic;Diameter;Disease;disease mechanisms study;Elasticity;Electrocardiogram;Ensure;Evaluation;Eye Movements;Feedback;Functional disorder;Generations;Geometry;Goals;Group Meetings;Health;Hematocrit procedure;Heterogeneity;Human;Hypertension;Image;Image Analysis;image processing;imaging modality;imaging Segmentation;Impaired cognition;improved;Indiana;innovation;insight;instrument;Intellectual Property;interest;Light;Mathematics;Measurement;Measures;Microscopic;millisecond;Molecular Conformation;Monitor;Morphologic artifacts;Network-based;novel;novel diagnostics;novel therapeutics;open source;Ophthalmoscopes;Optical Coherence Tomography;Optics;parallelization;Pericytes;Phase;Positioning Attribute;Process;Publications;real-time images;recruit;Research;Research Personnel;Retina;retinal imaging;Sampling;Scanning;Scientist;sharing platform;shear stress;Sickle Cell Anemia;Site;software development;Stream;Structure;Subcortical Infarctions;Subcortical Leukoencephalopathy;Systemic disease;Technology;Testing;Time;Training;Trees;Validation;venule;Wrist

PROJECT_TITLE: Cellular-level Vascular Oculomics (CVO) for monitoring systemic vascular health


ORG_CITY: PITTSBURGH

ORG_NAME: CARNEGIE-MELLON UNIVERSITY

ORG_STATE: PA

PHR: PROJECT NARRATIVE This proposal describes research to study how ribosomes are assembled in yeast and Hela cells, and how ribosome biogenesis is interconnected with the structure and function of the nucleolus, the cellular compartment where ribosomes are made. As the ribonucleoprotein machines that catalyze protein synthesis in all cells, ribosomes are essential for and closely linked to the growth, proliferation, and adaptation of cells. Consequently, dysregulation of ribosome synthesis or nucleolar structure results in cancers, anemias, neurodegenerative diseases, and a variety of developmental disorders.

PI_NAMEs: WOOLFORD, JOHN L. (contact)

PROGRAM_OFFICER_NAME: REDDY, MICHAEL K

PROJECT_TERMS: Address;Anemia;ATP phosphohydrolase;Automobile Driving;Biogenesis;cell growth;Cell Nucleolus;Cell Proliferation;Cells;Complex;developmental disease;Disease;Energy consumption;Eukaryota;experimental study;fluidity;genetic approach;Goals;Growth;Hela Cells;Human;in vivo;Learning;Link;Malignant Neoplasms;Molecular Genetics;Morphology;Nature;Neurodegenerative Disorders;Nucleoplasm;particle;Physical condensation;Production;Proliferating;Property;Protein Biosynthesis;Proteins;Regulation;Research;Ribonucleoproteins;Ribosomal Biogenesis Pathway;Ribosomal RNA;Ribosomes;RNA;RNA Helicase;Saccharomyces cerevisiae;Series;Structure;Visualization;Yeasts

PROJECT_TITLE: Yeast Ribosome Biogenesis


ADMINISTERING_IC: AT

IC_NAME: NATIONAL CENTER FOR COMPLEMENTARY & INTEGRATIVE HEALTH

NIH_SPENDING_CATS: Behavioral and Social Science;Chronic Pain;Clinical Research;Clinical Trials and Supportive Activities;Comparative Effectiveness Research;Complementary and Integrative Health;Health Disparities Research;Health Disparities and Racial or Ethnic Minority Health Research;Mental Health;Mental Illness;Neurosciences;Pain Research;Rehabilitation

ORG_CITY: SEATTLE

ORG_DEPT: PHYSICAL MEDICINE & REHAB

ORG_NAME: UNIVERSITY OF WASHINGTON

ORG_STATE: WA

PHR: Project Narrative While behavioral treatments for chronic pain are effective on average, some people benefit greatly from treatment while others benefit very little, and the same person may respond much more to one type of treatment than another. This project aims to better understand the patient factors that could be used to identify – before treatment –who will benefit the most from each of three different chronic pain treatments: cognitive behavioral therapy, Hypnotic Cognitive Therapy, and Mindfulness-Based Cognitive Therapy. The knowledge gained from this research will provide an essential platform for developing algorithms to effectively match patients with chronic pain to treatments that are most likely to be benficial for them.

PI_NAMEs: JENSEN, MARK P (contact);WILLIAMS, RHONDA

PROGRAM_OFFICER_NAME: BELFER, INNA

PROJECT_TERMS: Address;Affect;Aftercare;Algorithms;Behavior Therapy;Behavioral;Belief;Brain;chronic pain;chronic pain management;chronic pain patient;Clinical Trials;cognitive benefits;Cognitive Therapy;Complementary Health;coping;Data;design;Disabling;effective therapy;Effectiveness;Electroencephalography;Evidence based treatment;Foundations;Funding;Future;Health;Health Personnel;Hypnosis;hypnotic;improved;Individual;individual response;Integrative Medicine;Intervention;Knowledge;Measures;men;Mental Depression;mindfulness;mindfulness based cognitive therapy;Mindfulness Training;negative affect;Nonpharmacologic Therapy;Opioid Analgesics;optimism;Outcome Study;Pain;pain catastrophizing;Pain intensity;Pain management;pain outcome;parent project;Parents;Participant;Pathway interactions;Patients;Pattern;Perception;personalized care;Persons;pharmacologic;Play;predicting response;Predictive Factor;primary outcome;programs;Property;psychologic;Psychometrics;psychosocial;Quality of life;Questionnaires;Randomized;Research;Research Project Grants;Research Support;response;Self Perception;shared decision making;Sleep disturbances;standard care;Structure;Testing;theories;trait;treatment as usual;Treatment outcome;treatment response;Veterans;Woman

PROJECT_TITLE: Optimizing Response to Chronic Pain Treatments in Veterans: Identifying Key Moderators

STUDY_SECTION: BMHO

STUDY_SECTION_NAME: Biobehavioral Medicine and Health Outcomes Study Section[BMHO]


ADMINISTERING_IC: MD

IC_NAME: NATIONAL INSTITUTE ON MINORITY HEALTH AND HEALTH DISPARITIES

NIH_SPENDING_CATS: Behavioral and Social Science;Brain Disorders;Clinical Research;Clinical Trials and Supportive Activities;Comparative Effectiveness Research;Depression;Health Disparities Research;Health Disparities and Racial or Ethnic Minority Health Research;Mental Health;Mental Illness;Pediatric;Prevention;Racial and Ethnic Minority Health Research;Social Determinants of Health

ORG_CITY: Oakland

ORG_NAME: KAISER FOUNDATION RESEARCH INSTITUTE

ORG_STATE: CA

PHR: PROJECT NARRATIVE The proposed research will build upon prior community-driven and mixed methods research to evaluate the effectiveness of an online preventative parenting intervention intended to strengthen parent-child relationships among Filipinos, the third largest Asian subgroup in the United States. This research will impact the well-being of Filipino youth as well as society as a whole by expanding the cultural narratives and the evidence base supporting online parenting interventions during middle childhood.

PI_NAMEs: JAVIER, JOYCE RIVERA (contact)

PROGRAM_OFFICER_NAME: LINARES, DEBORAH ELIZABETH

PROJECT_TERMS: 12 year old;Address;Adolescent;Age;age group;Agreement;Anxiety;anxiety symptoms;Asian;Awareness;Behavior;behavioral health;behavioral outcome;California;Child;Child Behavior;child depression;Child Rearing;Childhood;Clinical;Communication;Communities;community organizations;community setting;Conflict (Psychology);depressive symptoms;Development;Discipline;Distress;early childhood;Effectiveness;effectiveness evaluation;effectiveness testing;Enrollment;evidence base;experience;experimental group;externalizing behavior;Face;Family;Family dynamics;Feeling suicidal;Filipino;Funding;Grant;health disparity;Hybrids;Immigrant;Immigrant family;Immigration;implementation facilitators;improved;Individual;intergenerational;Intervention;intervention delivery;Interview;Literature;maltreatment;Mental Depression;Mental disorders;middle childhood;Minority Groups;Modeling;National Center for Advancing Translational Sciences;National Institute of Child Health and Human Development;Not Hispanic or Latino;online intervention;Outcome;Parent-Child Relations;parenting intervention;Parents;Participant;Personal Satisfaction;Pilot Projects;Population Growth;prevent;Prevention program;Preventive;preventive intervention;primary outcome;Process;programs;protective factors;Protocols documentation;Provider;Publishing;Punishment;Qualitative Research;Randomized;Randomized, Controlled Trials;recruit;remote delivery;Reporting;Research;Research Methodology;resilience;Risk;Risk Factors;Sampling;School-Age Population;Scientific Advances and Accomplishments;secondary outcome;Secure;skills;skills training;social cognitive theory;Societies;Stress;Subgroup;substance use;suicidal behavior;theories;therapy design;Training Programs;treatment group;treatment trial;United States;usual care arm;verbal;virtual;Vulnerable Populations;Youth

PROJECT_TITLE: Virtual Positive Parenting Intervention to Promote Filipino Family Wellness: A Randomized Controlled Trial

STUDY_SECTION: HPC

STUDY_SECTION_NAME: Health Promotion in Communities Study Section[HPC]


ADMINISTERING_IC: AI

IC_NAME: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES

NIH_SPENDING_CATS: Behavioral and Social Science;Biotechnology;Clinical Research;Clinical Trials and Supportive Activities;Emerging Infectious Diseases;HIV/AIDS;Health Disparities Research;Health Disparities and Racial or Ethnic Minority Health Research;Immunization;Infectious Diseases;Prevention;Racial and Ethnic Minority Health Research;Rare Diseases;Social Determinants of Health;Tuberculosis;Vaccine Related;Vaccine Related - AIDS

ORG_CITY: CITY OF INDUSTRY

ORG_NAME: PUBLIC HEALTH FOUNDATION ENTERPRISES

PHR: Project Narrative The San Francisco Bay Clinical Trials Unit will conduct HIV vaccine, prevention and treatment trials in San Francisco with a broad community engagement reach throughout the San Francisco Bay area. By bringing together leading scientists and a diverse community in the San Francisco Bay Area, this research unit will continue to develop ways to prevent and treat HIV infections and work towards an effective HIV vaccine, new pre-exposure prophylaxis agents, multipurpose prevention technologies, new TB therapies, and an HIV cure.

PI_NAMEs: BUCHBINDER, SUSAN  (contact);HAVLIR, DIANE V

PROGRAM_OFFICER_NAME: COATES, CINDY KAREN

PROJECT_TERMS: Adherence;Affect;AIDS clinical trial group;AIDS prevention;Anti-Retroviral Agents;Antibodies;Area;Automobile Driving;behavioral and social science;Behavioral Sciences;Black Populations;Caring;clinical research site;Clinical Trials;Clinical Trials Network;Clinical Trials Unit;Communication;Communities;community engagement;data quality;design;Development;Disease remission;Drug user;efficacy trial;Enrollment;Ensure;Epidemic;Epidemiology;ethnic disparity;ethnic minority;experience;Generations;Goals;Grant;Grooming;Hepatitis;Hepatitis B;HIV;HIV diagnosis;HIV Infections;HIV vaccine;HIV Vaccine Trials Network;Home;improved;Infection;Inflammation;Infrastructure;Injectable;Injecting drug user;Journals;Latinx population;Leadership;Location;Manuscripts;men who have sex with men;Mentorship;neutralizing antibody;Output;Persons;Phase;Population;Population Heterogeneity;Positioning Attribute;pre-exposure prophylaxis;prevent;Prevention;Prevention Protocols;Prevention strategy;Prevention trial;Principal Investigator;Process;programs;Protocols documentation;Publications;racial disparity;racial minority;Records;recruit;Research;Research Infrastructure;Research Personnel;Role;Running;San Francisco;Science;Scientist;Site;social science research;Structure;Talents;Technology;Textiles;Training;training opportunity;transgender;Transportation;Treatment Protocols;treatment trial;Tuberculosis;tuberculosis treatment;Vaccine Research;vaccine trial;Vaccines;Vaginal Ring;Viral;Work;working group

PROJECT_TITLE: San Francisco Bay Clinical Trials Unit (CTU)


NIH_SPENDING_CATS: Genetics;Human Genome

ORG_NAME: BOSTON CHILDREN'S HOSPITAL

PHR: Contact PD/PI: Myong, Sua PROJECT NARRATIVE We propose to investigate the role of transcriptionally generated G-quadruplex (G4), R-loop and DNA supercoiling in transcription and translation. Potential G4 forming sequences are prevalent in regulatory regions of human and e. coli genome and the G4 formation can up or downregulate gene expression including many oncogenes. Defining the molecular mechanism will contribute to the development of rational cancer drugs. Project Narrative Page 7

PI_NAMEs: MYONG, SUA  (contact)

PROGRAM_OFFICER_NAME: CHOW, CHI-WING

PROJECT_TERMS: 5' Untranslated Regions;Antineoplastic Agents;biochemical tools;Cell-Free System;Cells;Development;DNA;Equipment;Escherichia coli;G-Quartets;Gene Expression;Genetic Transcription;Genome;Genomics;Goals;Human;Human Genome;Length;Measures;Messenger RNA;Molecular;Nucleic Acid Regulatory Sequences;Oncogenes;Output;plasmid DNA;Plasmids;Positioning Attribute;Process;Production;promoter;Proteins;Regulator Genes;Ribosomes;RNA;Role;single molecule;Slide;Structure;Structure-Activity Relationship;Superhelical DNA;System;Testing;Transcription Initiation Site;Translations

PROJECT_TITLE: Gene Expression Modulated by G4, R-loop and DNA Supercoiling (Supplement for Equipment)

STUDY_SECTION: MSFB

STUDY_SECTION_NAME: Macromolecular Structure and Function B Study Section[MSFB]


ADMINISTERING_IC: DK

IC_NAME: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES

NIH_SPENDING_CATS: Autoimmune Disease;Clinical Research;Crohn's Disease;Digestive Diseases;Genetics;Inflammatory Bowel Disease

ORG_CITY: MONTREAL

ORG_COUNTRY: CANADA

ORG_FIPS: CA

ORG_NAME: MONTREAL HEART INSTITUTE

ORG_STATE: PQ

ORG_ZIPCODE: H1T 1C8

PHR: Project Narrative This project aims to identify the genetic causes of the inflammatory bowel diseases (IBD) known as Crohn’s Disease (CD) and ulcerative colitis (UC), particularly in populations currently underrepresented in IBD genetic studies. We further aim to determine biological impact of the genetic risk factors to better understand disease processes.

PI_NAMEs: RIOUX, JOHN D. (contact)

PROGRAM_OFFICER_NAME: SHEA-DONOHUE, TEREZ

PROJECT_TERMS: African American population;Behavior;Biological;Biological Assay;Boston;Cell model;Chronic;Crohn's disease;Data Collection;Disease;disease phenotype;Ensure;Epithelium;experimental study;Gastrointestinal tract structure;Genes;Genetic;genetic architecture;Genetic Diseases;Genetic Research;genetic risk factor;Genetic study;Genetic Variation;Genomics;Hispanic Americans;human induced pluripotent stem cells;Inflammation;Inflammatory Bowel Diseases;member;Modeling;National Institute of Diabetes and Digestive and Kidney Diseases;Organoids;Patients;Population;Process;Relapse;Reporting;Research;RNA Splicing;Sampling;sex;Ulcerative Colitis;Variant

PROJECT_TITLE: Supplement in support of Diversity in Genetic studies of IBD and functional studies of IBD genes.


ADMINISTERING_IC: AG

IC_NAME: NATIONAL INSTITUTE ON AGING

NIH_SPENDING_CATS: Acquired Cognitive Impairment;Aging;Alzheimer's Disease;Alzheimer's Disease including Alzheimer's Disease Related Dementias (AD/ADRD);Basic Behavioral and Social Science;Behavioral and Social Science;Brain Disorders;Clinical Research;Dementia;Neurodegenerative;Neurosciences;Prevention

ORG_CITY: SAN FRANCISCO

ORG_NAME: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO

PHR: PROJECT NARRATIVE The number of individuals with clinical Alzheimer’s disease (AD) is rapidly increasing worldwide due to aging of the population, but current cognitive measures lack sufficient sensitivity and specificity to detect subtle decline within the preclinical phase. There is a critical need for tools that are sensitive to AD neuropathology during the preclinical phase that are low-cost, high-access, and fast to administer that can be used across educationally, linguistically, and culturally diverse older individuals. The goal of this project is to identify metrics for semantic impairment in diverse individuals that correlate with AD neuropathology and predict clinical decline.

PI_NAMEs: VONK, JET M.J. (contact)

PROGRAM_OFFICER_NAME: SUTTERER, MATTHEW

PROJECT_TERMS: Administrative Supplement;Age;age effect;Aging;Alzheimer's Disease;Alzheimer's disease diagnosis;Amyloid;Animals;apolipoprotein E-4;Area Under Curve;autosome;Biological Markers;Black race;brain volume;Canis familiaris;Caregivers;Categories;Clinical;clinical diagnosis;clinical predictors;Clinical Research;Cognitive;cognitive testing;cohort;cost;Data;Dementia;Demographic Factors;demographics;Diagnosis;diagnostic accuracy;early detection biomarkers;Early Diagnosis;Education;Episodic memory;Equation;Ethnic Origin;Foundations;Frequencies;Gender;gender difference;Genes;Genetic;Goals;high risk population;Hispanic;Iguanas;imaging biomarker;Impairment;Individual;Inherited;Intervention;Intervention Trial;Investigation;Knowledge;Language;Length;lexical;Linear Models;Linguistics;Longitudinal cohort;Magnetic Resonance Imaging;Maps;Measures;Memory impairment;Memory Loss;Modeling;mutation carrier;Names;neuroimaging marker;neuropathology;Neuropsychology;normal aging;novel;older adult;Older Population;Outcome;Participant;Patients;Performance;Persons;Phase;Population;Positron-Emission Tomography;pre-clinical;Predictive Value;Process;prognostic model;programs;Psycholinguistics;Race;Research;Risk Factors;Semantic memory;semantic processing;Semantics;Sensitivity and Specificity;sex;sociodemographics;Spanish;Spanish/English;Specificity;Statistical Data Interpretation;Symptoms;Testing;Thinness;Time;tool;trait;Translating;Universities;Variant;Walking;Work

PROJECT_TITLE: Administrative Supplement Preclinical markers of Alzheimer's disease using psycholinguistic semantic measures


ORG_DEPT: ANATOMY/CELL BIOLOGY

ORG_NAME: HARVARD MEDICAL SCHOOL

PHR: Project Narrative The goal of our research program is to understand the mechanisms that eliminate proteins from the cell. Because protein breakdown regulates important processes such as cell division, understanding these pathways may help us develop new treatments for diseases such as cancer.

PI_NAMEs: KING, RANDALL W (contact)

PROGRAM_OFFICER_NAME: PHILLIPS, ANDRE W

PROJECT_TERMS: Biological;Biology;Cell division;Cell Physiology;Cell Survival;Cells;Disease;drug development;enzyme substrate;Enzymes;Excision;experimental study;Genome;Goals;Health;Human;insight;interest;Knowledge;Malignant Neoplasms;Methods;Modernization;Modification;Molecular Target;multicatalytic endopeptidase complex;Mutation;new therapeutic target;Pathogenesis;Pathway interactions;Pharmaceutical Preparations;Process;programs;protein protein interaction;Proteins;Proteomics;Regulation;Regulatory Pathway;Research;Role;Signal Transduction;Specificity;Substrate Specificity;System;Ubiquitin;ubiquitin ligase;Ubiquitin-Conjugating Enzymes;Ubiquitination;virtual

PROJECT_TITLE: Mechanistic Analysis of the Ubiquitin-Proteasome System


ED_INST_TYPE: EARTH SCIENCES/RESOURCES

ORG_CITY: MERCED

ORG_NAME: UNIVERSITY OF CALIFORNIA, MERCED

PHR: PROJECT NARRATIVE Circadian clocks provide an accurate internal representation of local time under a range of diﬀerent physiological temperatures and cellular energy levels. However, the molecular mechanisms by which clocks compensate for such variations remain experimentally unresolved in any organism. Thus, the overall goal of this application is to ﬁll these fundamentally important knowledge gaps and thereby signiﬁcantly advance the ﬁeld of chronobiology with a lasting impact.

PI_NAMEs: LIWANG, ANDY  (contact)

PROGRAM_OFFICER_NAME: AKACEM, LAMEESE DANA

PROJECT_TITLE: Temperature and metabolic compensation mechanisms in a circadian clock system


FUNDING_MECHANISM: RESEARCH CENTERS

NIH_SPENDING_CATS: Bioengineering;Brain Cancer;Brain Disorders;Building Research Capacity at Institutions with Limited NIH Funding;Cancer;Emerging Infectious Diseases;Infectious Diseases;Nanotechnology;Neurosciences;Rare Diseases

ORG_CITY: CLEMSON

ORG_NAME: CLEMSON UNIVERSITY

ORG_STATE: SC

PI_NAMEs: LARSEN, JESSICA MARIE (contact)

PROGRAM_OFFICER_NAME: BERNAL, FEDERICO

PROJECT_TERMS: Amoeba genus;Binding;Blood;Blood - brain barrier anatomy;Brain;Brain Neoplasms;brain tissue;Bypass;Centers of Research Excellence;Central Nervous System Diseases;Death Rate;Eating;Encapsulated;enolase;Enzymes;Etiology;glucose metabolism;Homeostasis;Infection;Infiltration;inhibitor;innovation;Intranasal Administration;Invaded;Learning;Link;Meningitis;Modeling;Naegleria fowleri;nanoparticle;neural;Nose;opportunistic pathogen;pathogen;Pathogenicity;Pathology;Pathway interactions;Pharmaceutical Preparations;Plasminogen;Play;Polymers;prevent;Role;small molecule;Symptoms;therapy development;Trigeminal nerve structure;Viral

PROJECT_TITLE: Enabling Intranasal Therapies for Brain Invading Pathogens


ADMINISTERING_IC: DA

IC_NAME: NATIONAL INSTITUTE ON DRUG ABUSE

NIH_SPENDING_CATS: Behavioral and Social Science;Clinical Research;Clinical Trials and Supportive Activities;Dissemination and Implementation Research;Drug Abuse (NIDA only);Harm Reduction for Substance Misuse;Maternal Health;Maternal Morbidity and Mortality;Opioid Misuse and Addiction;Opioids;Pediatric;Perinatal Period - Conditions Originating in Perinatal Period;Pregnancy;Prevention;Recovery Support Services;Stimulant Use and Misuse;Substance Misuse;Women's Health Research

ORG_CITY: PORTLAND

ORG_NAME: OREGON HEALTH & SCIENCE UNIVERSITY

ORG_STATE: OR

PHR: Doucment not required.

PI_NAMEs: KORTHUIS, PHILIP TODD (contact)

PROGRAM_OFFICER_NAME: ZUR, JULIA BETH

PROJECT_TERMS: Abruptio Placentae;Address;Administrator;authority;Benchmarking;Birth;Cessation of life;Child;Child Welfare;Client;Collaborations;Communities;community engagement;community engagement process;Congenital Syphilis;Consolidated Framework for Implementation Research;contingency management;design;Documentation;Education;Employment;Excision;experience;Family;Goals;Harm Reduction;Health;Health Personnel;Housing;implementation strategy;Incentives;informant;innovation;Intervention;Interview;Lived experience;Manuals;Medical;Methods;Modeling;mortality;Opioid;opioid overdose;opioid use disorder;Oregon;Outcome;outreach;Overdose;patient oriented;peer;Perinatal;Persons;Policies;Postpartum Period;Pre-Eclampsia;Pregnancy;pregnant;Prevention;Process;programs;Psyche structure;Recovery Support;Recreation;Reporting;Risk;Risk Reduction;service providers;social;social stigma;Spirituality;stem;stillbirth;Stimulant;Stimulant overdose;stimulant use;stimulant use disorder;Structure;substance use;Substance Use Disorder;Suggestion;Testing;Time;Work

PROJECT_TITLE: PEER-CM Peripartum Supplement


NIH_SPENDING_CATS: Cardiovascular;Heart Disease

ORG_CITY: AUSTIN

ORG_NAME: UNIVERSITY OF TEXAS AT AUSTIN

PHR: PROJECT NARRATIVE. Leakage of the tricuspid valve, located between the right atrium and right ventricle, is extremely common and can significantly impact patients' health. We have recently found a new disease mechanism that may explain the failure of current treatment strategies. The goal of this supplement is to demonstrate that this disease mechanism can be targeted and suppressed with drugs and to pave the path for future pharmacological treatment strategies.

PI_NAMEs: RAUSCH, MANUEL KARL (contact)

PROGRAM_OFFICER_NAME: EVANS, FRANK

PROJECT_TERMS: American;Angiotensin II Receptor;Area;Cardiovascular Diseases;Cells;Clinical;computer studies;Disease;Disease Progression;Endothelial Cells;Endothelium;Extravasation;Failure;Future;Goals;Health;Heart Valves;improved;In Vitro;in vivo;inhibitor;interstitial cell;Losartan;Mediating;Mesenchymal;Mitral Valve;Operative Surgical Procedures;Outcome;parent grant;Patient-Focused Outcomes;Patients;Pharmaceutical Preparations;pharmacologic;Pharmacological Treatment;Phenotype;prevent;prophylactic;Renin-Angiotensin System;repaired;Right atrial structure;Right ventricular structure;sheep model;Stimulus;success;Testing;therapeutic target;treatment response;treatment strategy;Tricuspid Valve Insufficiency;Tricuspid valve structure;Work

PROJECT_TITLE: Tricuspid Valve Maladaptation: Its Stimuli, its Effect on   Valve Function, and its Response to Therapy

STUDY_SECTION: BTSS

STUDY_SECTION_NAME: Bioengineering, Technology and Surgical Sciences Study Section[BTSS]


NIH_SPENDING_CATS: Genetics;Rare Diseases

ORG_CITY: SAN ANTONIO

ORG_DEPT: GENETICS

ORG_NAME: UNIVERSITY OF TEXAS HLTH SCIENCE CENTER

PHR: Project Narrative Anti-cancer drugs and environmental chemicals elevate irreversible protein trapping in DNA, inducing a DNA damage response and mutagenesis if not repaired properly. The proposed research examines the key steps in cellular mechanisms that eliminate trapped proteins in DNA and thereby prevent the development of several motor neuron diseases and therapeutic resistance in breast and ovarian cancers.

PI_NAMEs: LEE, SANG EUN  (contact)

PROJECT_TERMS: Address;adduct;Aging;Alleles;anti-cancer;Antineoplastic Agents;APEXL2 Gene;Base Excision Repairs;Binding Proteins;Biochemical;Biochemical Genetics;Biochemistry;Biological;Biological Assay;Biological Models;Biological Process;BRCA deficient;BRCA1 gene;BRCA2 gene;Camptothecin;cancer cell;Cell Death;Cell Physiology;Cells;Chemicals;Chromatin;Collaborations;Complex;crosslink;Deoxyribonucleases;Development;Disease;Disease Resistance;DNA;DNA Adducts;DNA Damage;DNA lesion;DNA Repair;DNA Repair Pathway;DNA replication fork;DNA-(apurinic or apyrimidinic site) lyase;DNA-Directed DNA Polymerase;DNA-Directed RNA Polymerase;DNA-protein crosslink;environmental chemical;Environmental Pollutants;Enzymes;Etiology;Excision;Exposure to;Formaldehyde;Foundations;Gene Expression;Genetic;genetic approach;Genetic Recombination;Genome;Genomic Instability;Genomics;Growth;Histones;Homologous Gene;homologous recombination;Human;human disease;innovation;inorganic phosphate;Interruption;Investigation;Laboratories;Lymphopoiesis;Maintenance;Malignant Breast Neoplasm;Malignant neoplasm of liver;Malignant neoplasm of ovary;Modeling;Molecular Genetics;Motor Neuron Disease;Movement;multicatalytic endopeptidase complex;Mus;Mutagenesis;mutant;Mutation;Nerve Degeneration;nuclease;Nucleotide Excision Repair;Nucleotides;Pathway interactions;Patients;Peptide Hydrolases;Peptides;Periodicity;Physiological;prevent;Process;Property;protein complex;Proteins;Proteolysis;Reaction;receptor;reconstitution;Regulation;Repair Complex;repaired;Repetitive Sequence;Research;Resistance;response;Ribonucleotides;Role;seal;Single Strand Break Repair;Stress;Symptoms;Tail;Technical Expertise;Testing;Therapeutic;therapy resistant;Topoisomerase;Toxin;Type I DNA Topoisomerases;Tyrosine;Ubiquitin;Ubiquitination;Yeasts

PROJECT_TITLE: REPAIR OF DNA ENDS WITH ADDUCTS


ADMINISTERING_IC: MH

IC_NAME: NATIONAL INSTITUTE OF MENTAL HEALTH

ORG_CITY: BALTIMORE

ORG_DEPT: PSYCHIATRY

ORG_NAME: UNIVERSITY OF MARYLAND BALTIMORE

ORG_STATE: MD

PHR: A major NIMH goal for early psychosis treatment is to “prevent deterioration and disability among individuals suffering from psychotic illness”, however, rates of Social Security Administration disability (SSAD) enrollment remain high. Enrollment in SSI/DI may provide benefits (including cash assistance, access to Medicaid/Medicare and other social entitlements), but may detrimentally affect vocational aspirations and employment; these tradeoffs are even more pressing following the economic shocks of the COVID-19 crisis and heightened attention to racial. The goal of this project is to investigate factors influencing decisions to apply for and/or enroll in SSI/DI, the implications of these decisions, and the longitudinal relationships between SSI/DI and career development, and then use this information to develop systematic strategies for improving services, supporting client decision making and optimizing outcomes.

PI_NAMEs: GOLDMAN, HOWARD H (contact);JONES, GENEVRA

PROGRAM_OFFICER_NAME: FREED, MICHAEL C

PROJECT_TERMS: Affect;Age;aged;aspirate;Attention;Award;Black American;care recipients;care systems;career development;Characteristics;Client;Clinical;Communities;Complex;Consolidated Framework for Implementation Research;Coordinated Specialty Care;COVID-19 pandemic;Data;Data Collection;Data Set;Decision Making;design;Deterioration;Development;disability;Disability outcome;Disparity;early psychosis;Economics;Education;Employment;Enrollment;ethnoracial minority;Evaluation;evidence base;Evidence based practice;experience;Feedback;first episode psychosis;Funding;Generations;Goals;Impairment;implementation strategy;improved;improved outcome;Independent Living;Individual;individual variation;innovation;Interruption;Intervention;Interview;Ions;Knowledge;Learning;Link;Maps;Maryland;Medicaid;Medicare/Medicaid;Mental Health Services;Methods;minority children;Modeling;Motivation;National Institute of Mental Health;New York;Outcome;Partnership Practice;Pennsylvania;Persons;Phase;Play;Policies;Population;Poverty;prevent;Process;programs;Psychoses;Psychotic Disorders;Race;racial difference;racial discrimination;racial disparity;racial identity;Recovery;Research;Research Personnel;research to practice;Role;Schizophrenia;Schools;Security;Service delivery model;Services;Shock;Site;social;social determinants;Social Security;sociodemographics;Structural Racism;Surveys;Texas;Time;Translational Research;United States;United States Social Security Administration;Vocation;Work;young adult;Youth

PROJECT_TITLE: Optimizing Disability Benefit Decisions and Outcomes in First Episode Psychosis

STUDY_SECTION: SERV

STUDY_SECTION_NAME: Mental Health Services Study Section[SERV]


NIH_SPENDING_CATS: Complementary and Integrative Health;Nutrition

ORG_DEPT: PHARMACOLOGY

ORG_NAME: UNIVERSITY OF PITTSBURGH AT PITTSBURGH

PHR: Project Narrative The acidic gastric compartment promotes efficient nitration of dietary conjugated fatty acid when consumed with nitrite and nitrate-rich foods (e.g., leafy vegetables), forming nitrated fatty acids that exert protective and anti-inflammatory actions. We recently defined nitrated conjugated linolenic acid as a major nitro fatty acid species in humans and propose to study their mechanism of formation and signaling actions. This will provide dietary approaches to metabolic and inflammatory diseases.

PI_NAMEs: SCHOPFER, FRANCISCO JOSE (contact)

PROGRAM_OFFICER_NAME: BARSKI, OLEG

PROJECT_TERMS: absorption;animal tissue;Anti-Inflammatory Agents;Biological;Consumption;Dairy Products;dietary approach;Dietary Component;Dietary Fatty Acid;dietary nitrate;Disease;Event;Fatty Acids;Food;Heat shock proteins;Human;human tissue;Inflammation;Inflammatory;Linolenic Acids;Lipids;Liquid substance;Mediator;Metabolic;Metabolic Diseases;Metabolism;Nitrates;nitration;Nitrites;Nitrogen Dioxide;Pathologic;Pharmacology;Plants;Property;protective effect;protein expression;Proteins;Reaction;Role;Signal Transduction;Site;Stomach;Testing;Urine;Vegetables;western diet

PROJECT_TITLE: Formation and metabolism of nitrated fatty acids


ORG_CITY: Columbus

ORG_DEPT: PHYSIOLOGY

ORG_NAME: OHIO STATE UNIVERSITY

ORG_STATE: OH

PHR: Project Narrative No Changes

PI_NAMEs: CHINTHALAPUDI, KRISHNA  (contact)

PROGRAM_OFFICER_NAME: AINSZTEIN, ALEXANDRA M

PROJECT_TERMS: non-muscle myosin;Structure

PROJECT_TITLE: Structure and function of nonmuscle myosins

STUDY_SECTION: MSFC

STUDY_SECTION_NAME: Macromolecular Structure and Function C Study Section[MSFC]


ORG_CITY: Aurora

ORG_DEPT: BIOCHEMISTRY

ORG_NAME: UNIVERSITY OF COLORADO DENVER

ORG_STATE: CO

PHR: PROJECT NARRATIVE Noncoding RNAs are major players in the programming of gene expression in development and disease. Our research program uncovers how this programming is initiated at the molecular level and develops fundamental models to study other similar mechanisms that are dysregulated in disease. We dissect key interactions in these pathways to determine how to correct aberrant gene programming.

PI_NAMEs: JOHNSON, AARON M. (contact)

PROJECT_TERMS: Address;Base Pairing;Biochemical;Biological;Cell Nucleus;Cells;Centromere;Chromatin;Development;Disease;Epigenetic Process;Equipment;Gene Expression;Gene Expression Profile;Gene Expression Regulation;gene repression;Genes;Genome Stability;Genomic approach;Goals;Heritability;Heterochromatin;Histones;Human;human disease;Human Genome;insight;Intervention;Junk DNA;Link;Malignant Neoplasms;Mediating;Messenger RNA;Modeling;Molecular;Molecular Mechanisms of Action;Nuclear;Pathway interactions;pharmacologic;piRNA;programs;Public Health;Reader;Research;RNA;RNA-Binding Proteins;Study models;System;Transcript;Untranslated RNA;Work

PROJECT_TITLE: Equipment Supplement - Johnson R35GM144358


ORG_CITY: UNIVERSITY PARK

ORG_NAME: PENNSYLVANIA STATE UNIVERSITY, THE

PHR: Project Narrative Genomic basis of phenotypic variability of genomic disorders - Supplement This proposal is to request for supplemental funding to upgrade a confocal microscope from version LSM 700 to version LSM 900. Improving our microscopy capability will allow us to obtain higher resolution images from our experiments of Drosophila eyes and brain to test the molecular effects of individual 16p12.1 homologs and their genetic interactions. The newer version will also decrease the analysis time, and this equipment will be shared with the other NIGMS-funded investigator (Dr. Rolls) who will be partially covering the costs of the upgrade.

PI_NAMEs: GIRIRAJAN, SANTHOSH  (contact)

PROGRAM_OFFICER_NAME: MASKERI, BAISHALI

PROJECT_TERMS: 16p11.2;Biological Assay;Brain;cell type;Child;Chromosome Mapping;Chromosomes;Clinical;cohort;Complex;cost;Counseling;Defect;Development;Developmental Delay Disorders;Disease;Drosophila eye;Drosophila genus;Drosophila melanogaster;Equipment;experimental study;Family;Family history of;Funding;Gene Combinations;Generations;Genes;Genetic;genetic approach;genetic disorder diagnosis;Genome;genome sequencing;Genomics;Goals;high resolution imaging;Homologous Gene;improved;Individual;Inherited;knock-down;Lead;Microscope;Microscopy;Modeling;Molecular;Mutation;National Institute of General Medical Sciences;Neurologic;Neurons;neuropsychiatric disorder;neuropsychiatry;Outcome;Parents;Partner in relationship;Patients;Pattern;Phenotype;Population;Predisposition;proband;rare variant;Recording of previous events;Research Personnel;Risk;Sample Size;Severities;Testing;therapeutic development;Time;Variant;whole genome;Xenopus laevis;Zebrafish


NIH_SPENDING_CATS: Genetics;Prevention

ORG_CITY: Nashville

ORG_NAME: VANDERBILT UNIVERSITY

ORG_STATE: TN

PHR: PROJECT NARRATIVE This application seeks to understand how the replication stress response promotes completion of DNA synthesis each cell division cycle while maintaining genome stability. Genome instability causes many diseases including developmental disorders and cancer. Therefore, completing this project will provide insights into basic genome maintenance mechanisms with significant clinical application.

PI_NAMEs: CORTEZ, DAVID K (contact)

PROJECT_TERMS: Back;Base Pairing;Binding;Biochemical;Biological;biological adaptation to stress;Cell Cycle;Cells;clinical application;developmental disease;Disease;DNA;DNA biosynthesis;DNA Damage;DNA lesion;DNA replication fork;Excision Repair;Funding;Future;Genetic;Genetic Transcription;Genome;Genome Stability;Genomic Instability;Health;Human;insight;Knowledge;Lesion;Maintenance;Malignant Neoplasms;Modeling;nuclease;Positioning Attribute;prevent;Rad51 recombinase;Reagent;repaired;replication stress;response;SS DNA BP;Stress;Testing;Translating;translocase

PROJECT_TITLE: Analysis of the Replication Stress Response

STUDY_SECTION: MG

STUDY_SECTION_NAME: Molecular Genetics Study Section[MG]


NIH_SPENDING_CATS: Clinical Research;Clinical Trials and Supportive Activities;Diabetes;Infectious Diseases;Microbiome;Prevention;Wound Healing and Care

ORG_CITY: ANN ARBOR

ORG_NAME: UNIVERSITY OF MICHIGAN AT ANN ARBOR

ORG_STATE: MI

PHR: PROJECT NARRATIVE This project has major public health relevance because diabetic foot ulcers (DFU) remain one of the most common secondary diabetes-related complications and are the leading cause of lower extremity amputation in the United States. Current treatments are ineffective and evidence now demonstrates a surge in major lower extremity amputations and necessitates development of novel treatment avenues and technologies to advance care for this vulnerable population. The main objective of this proposal is to utilize metagenomics next generation sequencing to understand the role of the microbiome in the pathogenesis of DFU and identify new treatments and reduce sequela of DFU.

PI_NAMEs: SCHMIDT, BRIAN M (contact)

PROGRAM_OFFICER_NAME: LI, YAN

PROJECT_TERMS: Address;Adult;Affect;American;Amputation;Antibiotic Therapy;Award;Bacteria;biobank;Biological Markers;biomarker validation;Biometry;candidate identification;career development;Caring;chronic ulcer;Clinical;clinical care;Clinical Research;clinical research site;clinical trial implementation;Clinical Trials;cohort;Communicable Diseases;Complications of Diabetes Mellitus;Consensus;Controlled Study;Cross-Sectional Studies;Data Coordinating Center;Death Rate;Debridement;Dependence;design;Development;Development Plans;diabetes control;Diabetes Mellitus;Diabetic Foot;Diabetic Foot Ulcer;Ecology;Event;evidence base;experience;Foundations;Functional disorder;Future;Genetic Materials;Goals;healing;Health;improved;improved outcome;Individual;ineffective therapies;Infection;Infrastructure;Interruption;Intervention;Intervention Studies;Investigation;Laboratories;Leadership;Longitudinal Studies;Lower Extremity;Mentors;Metagenomics;Michigan;microbial;microbiome;microbiota;microbiota composition;modifiable risk;mortality;National Institute of Diabetes and Digestive and Kidney Diseases;Neuropathy;new technology;next generation sequencing;non-diabetic;novel;Observational Study;pathogen;Pathogenesis;patient oriented;Patients;Performance;Physicians;Physiological;Physiology;Podiatry;Positioning Attribute;Practice Guidelines;prevent;Process;programs;public health relevance;Public Health Schools;Research;Research Methodology;Research Personnel;Risk;Risk Factors;risk stratification;Role;Scientific Advances and Accomplishments;Scientist;skills;standard of care;Standardization;Sterile coverings;synergism;targeted treatment;Techniques;Technology;Testing;Therapeutic;tool;Training;United States;Universities;University resources;validation studies;Variant;Vulnerable Populations;wound;wound healing

PROJECT_TITLE: The Role of the Microbiome in Diabetic Foot Ulcers

STUDY_SECTION: DDK

STUDY_SECTION_NAME: Diabetes, Endocrinology and Metabolic Diseases B Study Section[DDK(M1)-1-B]


NIH_SPENDING_CATS: Genetics;Microbiome

ORG_CITY: WORCESTER

ORG_NAME: UNIV OF MASSACHUSETTS MED SCH WORCESTER

PHR: PROJECT NARRATIVE Bacteria live everywhere as members of multispecies communities, and the activities in these microbiomes can have profound impacts on their hosts as well as the environment. This research focuses on discovering the chemical and genetic mechanisms bacteria use to interact with one another. Gaining a molecular understanding of these cell-cell interactions will provide us with a better understanding of microbial ecology as well as chemical tools to manipulate bacterial behavior in natural microbiomes.

PI_NAMEs: SHANK, ELIZABETH ANNE (contact)

PROGRAM_OFFICER_NAME: BOND, MICHELLE RUEFFER

PROJECT_TERMS: Animals;Antibiotics;Bacillus subtilis;Bacteria;bacterial community;Bacterial Physiology;Behavior;Biological Models;Cell Communication;Cells;Chemicals;Communities;Complex;Cues;Development;Ecology;Environment;extracellular;Genetic;Genetic Transcription;Heterogeneity;interest;Intervention;member;Metabolic;Metabolism;Microbe;microbial;Microbial Biofilms;microbial community;microbiome;Molecular;National Institute of General Medical Sciences;Nature;Phylogenetic Analysis;Physiology;Planets;Plants;Play;Prevalence;Probiotics;Research;Role;secondary metabolite;Shapes;Signal Transduction;Soil;Source;Spatial Distribution;Specificity;Swimming;System;Therapeutic;tool

PROJECT_TITLE: Investigating the molecules and mechanisms of bacterial cell-cell interactions

STUDY_SECTION: MRAA

STUDY_SECTION_NAME: Maximizing Investigators? Research Award A Study Section[MRAA]


ED_INST_TYPE: SCHOOLS OF OSTEOPATHIC MEDICINE

ORG_CITY: EAST LANSING

ORG_DEPT: MICROBIOLOGY/IMMUN/VIROLOGY

ORG_NAME: MICHIGAN STATE UNIVERSITY

PHR: Project Narrative. This Administrative Supplement request funds for the purchase of an Agilent BioTek LogPhase 600 microbiology plate reader through Notice NOT-GM-24-021 under the parent grant R35 GM139537, “Exploring cyclic di-nucleotide signaling across the tree of life”. This plate reader will greatly enhance our ability to perform microbial growth curves and phage infection assays, both of which are central to research supported by the grant.

PI_NAMEs: WATERS, CHRISTOPHER M (contact)

PROJECT_TERMS: Administrative Supplement;Award;Bacteria;Bacterial Physiology;Bacteriophages;Biological Assay;Cells;Clustered Regularly Interspaced Short Palindromic Repeats;cost;Cytolysis;density;Devices;Dinucleoside Phosphates;DNA;Drops;Equipment;experimental study;Funding;Grant;Growth;Infection;Investments;Kinetics;Laboratories;Life;Measures;member;microbial;Microbiology;Molecular;Optics;parent grant;Periodicity;Phase;Phenotype;Population;Publications;Reader;Research;Research Support;restriction enzyme;Signal Transduction;System;Time;tool;Trees;United States National Institutes of Health;Virus

PROJECT_TITLE: Exploring cyclic di-nucleotide signaling across the tree of life (Supplement)


ORG_CITY: SYRACUSE

ORG_NAME: UPSTATE MEDICAL UNIVERSITY

PHR: Project Narrative This project seeks to develop proteins and enzymes that are switched on and off by binding of specific DNA or RNA sequences, as well as by proteins and small molecules. These protein switches will be used as biosensors for detecting pathogens and disease markers, and to selectively kill virally-infected cells while leaving uninfected cells unharmed.

PI_NAMEs: LOH, STEWART N (contact)

PROGRAM_OFFICER_NAME: SIMONOVIC, MILJAN

PROJECT_TERMS: Acceleration;Address;Antibodies;aptamer;Bacillus amyloliquefaciens ribonuclease;Base Sequence;BCAR1 gene;Binding;Biological;Biological Markers;Biology;Biophysics;Biosensor;Bone Marrow Stem Cell;Calcium;Calcium Signaling;Calmodulin;Cause of Death;Cells;Cellular Phone;Clustered Regularly Interspaced Short Palindromic Repeats;combinatorial;Complex;Computing Methodologies;Cytomegalovirus;cytotoxic;design;Devices;Disease;Disease Marker;DNA;DNA Binding;DNA Binding Domain;DNA Sequence;Engineering;Enzymes;Exhibits;experimental study;Family;Fibronectins;Fluorescence;Fluorescence Resonance Energy Transfer;frontier;gene editing technology;Genes;Goals;Guide RNA;Human;Human Activities;Immunocompromised Host;Individual;Kinetics;Ligand Binding;Ligands;Ligation;link protein;luminescence;Mediating;Methodology;Modification;Molecular Conformation;Monitor;monocyte;Morbidity - disease rate;mortality;nanoluciferase;Nature;Nucleic Acid Binding;nucleic acid binding protein;Nucleic Acids;Outcome;Output;parent grant;pathogen;Pathogen detection;Pathway interactions;point-of-care detection;prevent;Protein Conformation;Protein Engineering;Protein Family;protein folding;protein function;Proteins;Proteome;ratiometric;Reaction Time;Regulation;Research;response;Ribonucleases;RNA;RNA Binding;RNA Sequences;scaffold;sensor;simulation;small molecule;Source;System;Technology;Therapeutic;tool;Toxin;Transplant Recipients;Transplantation;Variant;Viral

PROJECT_TITLE: Combining protein and DNA engineering to create bioswitches


NIH_SPENDING_CATS: Biotechnology;Clinical Research;Hematology;Nutrition;Obesity;Wound Healing and Care

PHR: Targeting neutrophils to harness myeloid responses for wound healing Project narrative: Chronic wounds represent a significant health problem in the United States with millions of afflicted patients and the associated treatment billions of dollars per year. In the proposed research, we will investigate how chronic wounds and associated obesity dysregulate white blood cells that contribute to the poor healing responses in these conditions. Findings from our experiments will provide insight into whether therapies and tests targeting a type of white blood cells can improve the healing of wounds and can aid in the selection of treatment options that are currently available for treating chronic wounds. The long-term goal of this project is to reduce the socioeconomic burden of chronic wounds and associated obesity and improve the quality of life of these patients.

PI_NAMEs: URAO, NORIFUMI  (contact)

PROGRAM_OFFICER_NAME: CHAO, CHIEN-CHUNG

PROJECT_TERMS: Adult;alpha-glycerophosphoric acid;assay development;Biochemical;Biological;Biological Assay;Biological Markers;Biomedical Engineering;Blood;Bone Marrow;Carrier Proteins;cell behavior;Cell Culture System;Cell Culture Techniques;Cell Death;cell growth;Cell Survival;Cells;Characteristics;chronic wound;Citrates;cloud based;Computer software;controlled environment chamber;cost;Culture Media;culture plates;Cultured Cells;Data;Data Analytics;Data Storage and Retrieval;Diabetes Mellitus;Disease;Disparity;Drug Targeting;Dyes;Economic Burden;Environment;Environmental Risk Factor;Equipment;equipment acquisition;experimental study;extracellular vesicles;Faculty;fluorescence imaging;Fluorescent Dyes;Funding;Future;Gases;Glucose;Glutaminase;Glutamine;Goals;Grant;healing;Health;Human;human subject;Hypoxia;Image;Immune;Immune system;Impaired wound healing;improved;In Vitro;in vivo;Inflammation;Inflammatory;inhibitor;insight;Institutional Review Boards;Knowledge;Label;Leukocytes;Link;Macrophage;Macrophage Activation;Mammalian Cell;Mechanics;Metabolic;Metabolic Pathway;Metabolism;Methods;Microscope;microscopic imaging;Mitochondria;Modeling;Molecular;Monitor;mouse model;Myelogenous;Myeloid Cells;Myelopoiesis;nanodrug;neutrophil;Nutrient;Obesity;Organ;Organism;Outcome Study;Oxygen;Parents;Partial Pressure;Patients;Pentosephosphate Pathway;Pharmaceutical Preparations;pharmacologic;Phenolsulfonphthalein;Phenotype;Physiological;Plasma;pre-clinical;preclinical study;Process;Proliferating;Protocols documentation;Quality of life;Reagent;Recurrence;regenerative;repaired;Research;Research Personnel;response;Role;Salts;Sampling;Scientist;Selection for Treatments;Serum;Shock;skills;socioeconomics;Specimen;Stains;success;Supporting Cell;Techniques;Temperature;Testing;Time;Tissues;Training;translational potential;translational study;United States;Update;uptake;Vendor;wound;wound healing;wound response

PROJECT_TITLE: Targeting neutrophil clearance to harness myeloid responses for wound healing.

STUDY_SECTION: SAT

STUDY_SECTION_NAME: Surgery, Anesthesiology and Trauma Study Section[SAT]


ORG_CITY: HOUSTON

ORG_NAME: BAYLOR COLLEGE OF MEDICINE

ORG_STATE: TX

PHR: PROJECT NARRATIVE Unlike the static, double-helical structure of DNA depicted in textbooks, sculpture, and art, the DNA of living cells is dynamic—winding and unwinding to allow its duplication and repair. Many antibiotic and anticancer drugs target enzymes that bind to DNA only in underwound or overwound states, yet the impact of DNA winding has been largely ignored because the tools to address it properly in experiments did not exist. To remedy this, we invented methods to create DNA as it exists in living cells and propose to study how these drugs work and find new drugs for infections, cancer, and neurological diseases.

PI_NAMEs: ZECHIEDRICH, LYNN  (contact)

PROGRAM_OFFICER_NAME: SAKALIAN, MICHAEL

PROJECT_TERMS: Address;Affect;analytical ultracentrifugation;Antibiotics;antimicrobial;Antineoplastic Agents;Binding;Biochemical;Biological Assay;Biophysics;Cell Physiology;Cells;Chromatin Loop;clinical application;Coupled;Cryoelectron Microscopy;Data;design and construction;DNA;DNA biosynthesis;DNA Structure;DNA Topoisomerases;drug action;drug efficacy;Drug Interactions;Drug Targeting;Engineering;Enzymes;Equipment;experimental study;Fluorescence;Frequencies;fundamental research;Gene Delivery;gene therapy;Gene Transduction Agent;Genetic Transcription;Genomic Instability;Gold;Human;human disease;Imaging Device;improved;in vivo;Infection;inhibitor;innovation;insight;invention;Knowledge acquisition;Libraries;Malignant Neoplasms;Methods;Molecular Conformation;Monosaccharides;nanoparticle;Nervous System Disorder;novel;novel therapeutics;Pharmaceutical Preparations;Physiological;Property;repaired;Research;screening;Sculpture;Shapes;Structure;Superhelical DNA;targeted agent;Techniques;Textbooks;Therapeutic;therapeutic target;three dimensional structure;Time;tomography;tool;Topoisomerase;uptake;Vision;Work

PROJECT_TITLE: 2024 Equipment: Harnessing Supercoiling to Regulate DNA Activity

STUDY_SECTION_NAME: Special Emphasis Panel[ZRG1(55)-R]


NIH_SPENDING_CATS: Bioengineering;Biomedical Imaging;Machine Learning and Artificial Intelligence;Networking and Information Technology R&D (NITRD);Patient Safety;Physical Injury - Accidents and Adverse Effects;Prevention

ORG_DEPT: RADIATION-DIAGNOSTIC/ONCOLOGY

PHR: PROJECT NARRATIVE: Up to 86% of preventable deaths after trauma are related to sequelae of multicavitary hemorrhage in the non-compressible torso. Whole-body CT angiography (WBCTA) is routinely performed on trauma admission as a screening and surgical planning tool and is associated with a survival benefit, however interpretation delays contribute to delays to transfusion and surgical or angiographic hemostatic intervention. High-trust human-centered computer-aided detection and diagnosis (CAD) tools for screening, hemorrhage burden quantification, and personalized decision support will accelerate and standardize image analysis of torso hemorrhage.

PI_NAMEs: DREIZIN, DAVID  (contact)

PROJECT_TERMS: Acceleration;Acidosis;Admission activity;Angiography;Blood Coagulation Disorders;Cessation of life;Classification;Clinical;clinical decision-making;clinically relevant;Communities;computer aided detection;computer center;Computer-Assisted Diagnosis;Data Set;Detection;Diagnosis;diagnostic tool;Early Intervention;Engineering;Ensure;experience;Extravasation;Future;Goals;Hematoma;Hemorrhage;Hemostatic Agents;Hemothorax;Human;human centered design;Image;Image Analysis;image processing;improved;indexing;Injury;Intervention;Laceration;large datasets;Life;Measurement;Medical Imaging;Modality;mortality risk;Multi-Institutional Clinical Trial;Multiple Trauma;natural hypothermia;Operative Surgical Procedures;Organ;Outcome;Patient Triage;Pelvis;pelvis fracture;personalized decision;personalized diagnostics;point of care;Population;Population Heterogeneity;preventable death;Protocols documentation;Radiology Specialty;Reporting;Safety;Sampling;screening;Screening procedure;Severities;Shock;Software Design;Standardization;Teleradiology;Time;tool;Transfusion;Trauma;trauma care;trauma centers;Trust;United States;Work

PROJECT_TITLE: Human-centered CT-based CADx Tools for Traumatic Torso Hemorrhage

STUDY_SECTION: CTIS

STUDY_SECTION_NAME: Clinical Translational Imaging Science Study Section[CTIS]


NIH_SPENDING_CATS: Basic Behavioral and Social Science;Behavioral and Social Science;Bioengineering;Complementary and Integrative Health;Genetics;Neurosciences;Nutrition

ORG_CITY: HONOLULU

ORG_NAME: UNIVERSITY OF HAWAII AT MANOA

ORG_STATE: HI

PHR: Stereotypic repetitive behaviors, generally thought of as non-functional, are seen in a wide range of vertebrate species in blood glucose-inducing stress-associated environments, and are reduced under the ketosis-related nutritional condition. Accordingly, the short-term goals of this research program are, by using the requested microscope, to address the active neurons associated with repetitive behaviors in an experimental fish model that implemented naturalistic heterogeneity in the genome and behaviors. The long- term goals for this project are to reveal the genetic and neural mechanisms of how repetitive behavior is chosen among other types of voluntary behaviors, particularly in terms of the interactions between stress- associated and nutritional conditions.

PI_NAMEs: IWASHITA, MOTOKO ;YOSHIZAWA, MASATO  (contact)

PROGRAM_OFFICER_NAME: STEPANEK, LAURIE ELIZABETH

PROJECT_TERMS: 3-Dimensional;Address;Algorithmic Analysis;Animal Genetics;Animals;Behavior;behavior prediction;Behavioral;Biological Models;Blood Glucose;Brain;Complex;Corpus striatum structure;CRISPR/Cas technology;Data;Data Analyses;Department chair;Development;Dietary Intervention;distraction;effective therapy;Environment;Equipment;Exhibits;Experimental Models;Fishes;fluorescence microscope;Future;Gene Expression;Genes;Genetic;Genome;Genomics;Goals;Grant;Hearing;Heterogeneity;Hour;Human;Hyperactivity;Image;Immediate-Early Genes;Inbred Mouse;insight;Involuntary Movements;ketogenic diet;Ketone Bodies;Ketosis;Knowledge;Mammals;Maps;metabolomics;Methods;Mexican;Microscope;model organism;Modeling;Modernization;Molecular;Morphology;multidimensional data;Nervous System;Nervous System Physiology;neural;Neural Pathways;neuromechanism;Neurons;null mutation;nutrition;Nutritional;open data;Optics;Output;Pathway interactions;Patients;Periodicity;Photobleaching;Physiological;Population;programs;Quantitative Trait Loci;Recording of previous events;repetitive behavior;Research;response;Sampling;Scanning;sensor;Sensory;Serum;Signal Transduction;Speed;Spottings;Standard Model;Stereotyping;Stress;Structure;Surface;Surveys;System;Techniques;Technology;Testing;Three-Dimensional Imaging;transcriptome;transcriptomics;Ultraviolet Rays;Variant;Visualization;Wild Animals

PROJECT_TITLE: Gene Coexpression Network Regulating Repetitive Behavior under Nutritional Change.

STUDY_SECTION: BRLE

STUDY_SECTION_NAME: Biobehavioral Regulation, Learning and Ethology Study Section[BRLE]


NIH_SPENDING_CATS: Clinical Research;Hematology;Infectious Diseases;Machine Learning and Artificial Intelligence;Networking and Information Technology R&D (NITRD);Prevention;Sepsis

ORG_CITY: Oakland

ORG_NAME: KAISER FOUNDATION RESEARCH INSTITUTE

PHR: PROJECT NARRATIVE Sepsis kills 11 million people every year and early identification and treatment are the only known ways to improve mortality. We currently focus on treating people once they get to the hospital or emergency department, which is often too late. This study will evaluate opportunities to identify and triage patients who may be at high-risk of sepsis before they get to the hospital, informed by a wide array of clinical data.

PI_NAMEs: LIU, VINCENT  (contact)

PROGRAM_OFFICER_NAME: ZHAO, XIAOLI

PROJECT_TERMS: Accident and Emergency department;Address;Architecture;Caring;Cessation of life;Clinical Data;cost;Data;deep learning;design;Early identification;Early treatment;Electronic Health Record;Equipment;Equity;frontier;Future;Goals;Health system;high risk;Hospital Departments;Hospitals;Image;improved;improved outcome;Infection;Informatics;innovation;Intervention;Intervention Studies;large language model;machine learning method;mortality;novel;open source;Patient Triage;Patients;Persons;predictive modeling;prevent;programs;prospective;Public Health;Research;Sepsis;septic patients;Series;Speed;Techniques;Text;Time;treatment guidelines

PROJECT_TITLE: Identifying pre-sepsis opportunities for early, targeted intervention - Equipment Supplement

STUDY_SECTION_NAME: Special Emphasis Panel[ZRG1(01)-Q]


NIH_SPENDING_CATS: Biotechnology;Genetics

ORG_CITY: STANFORD

ORG_NAME: STANFORD UNIVERSITY

PHR: Narrative / Relevance (parent grant) Our proposed research is aimed at developing new chemical tools for analyzing the interactions and functions of messenger RNAs in the cell, and applying them to address important biomedical questions. Our new methods will establish easy-to-install chemical handles on large, complex RNAs that allow them to be labeled, tracked, analyzed, and controlled. We will use them to investigate how drugs and metabolites interact with RNAs in the cell, and to study the effects of chemical modifications that are included in mRNA vaccines and upcoming RNA therapies.

PI_NAMEs: KOOL, ERIC T. (contact)

PROGRAM_OFFICER_NAME: ASLAN, KADIR

PROJECT_TERMS: Address;Affect;base;Binding Sites;Biological;Biology;Cells;Chemicals;Chemistry;Clinical;clinically relevant;Complex;Drug Interactions;Equipment Failure;Future;Goals;Health;Human;improved;insight;interest;Label;Ligand Binding;Ligands;Light;Mass Spectrum Analysis;Mediating;Messenger RNA;Metabolite Interaction;Methodology;Methods;Modification;Molecular;next generation;next generation sequencing;novel;novel strategies;parent grant;parent project;Pathway interactions;Pharmaceutical Preparations;Process;programs;Proteins;Proteome;Pump;Reagent;Research;Research Personnel;RNA;RNA analysis;RNA Binding;RNA vaccine;Sampling;secondary metabolite;Site;small molecule;Structure;Surveys;System;Technology;Therapeutic;Therapeutic Agents;therapeutic RNA;therapeutic target;tool;Toxic effect;transcriptome;Universities;Vacuum;virtual;Work

PROJECT_TITLE: Transcriptome Analysis with RNA-Reactive Probes


NIH_SPENDING_CATS: Cardiovascular;Clinical Research;Heart Disease;Kidney Disease;Prevention

ORG_CITY: BOSTON

ORG_NAME: BRIGHAM AND WOMEN'S HOSPITAL

PHR: PROJECT NARRATIVE  Cardiac surgery-associated acute kidney injury (CSA-AKI) is a major public health burden, with no therapy that reliably prevents or treats it. Abundant data from animal models and humans suggest that hepcidin and other heme/iron regulatory proteins play a key role in CSA-AKI, and are being investigated in the parent R01 award. In this diversity supplement, we will investigate whether elevated levels of the hormone, erythropoietin, which regulates red blood cell production as well as hepcidin production, is an early marker of CSA-AKI.

PI_NAMEs: LEAF, DAVID EVAN (contact)

PROGRAM_OFFICER_NAME: SCHULMAN, IVONNE HERNANDEZ

PROJECT_TERMS: Acute;Acute Renal Failure with Renal Papillary Necrosis;Adult;Affect;Agonist;animal data;Animal Model;Attenuated;Award;biobank;Boston;Cardiac Surgery procedures;Cardiac Volume;Cardiopulmonary Bypass;Case/Control Studies;cell type;Circulation;Clinical Data;Clinical Trials;Complex;Critical Illness;Data;design;Development;Enrollment;Erythrocytes;Erythropoietin;experience;Ferritin;Flow Cytometry;Future;genetic regulatory protein;Genomics;Haptoglobins;Heme;Hemoglobin;Hemolysis;Hemopexin;Hepatocyte;hepcidin;high risk;Homeostasis;Hormones;Hospitals;Human;human data;Incidence;Inflammation;Infrastructure;injury prevention;innovation;Interleukin-6;Investigation;Iron;Iron Metabolism Disorders;Iron-Regulatory Proteins;Measures;metal transporting protein 1;Modeling;monocyte;mortality;North America;novel;oxidative damage;parent grant;Parents;participant enrollment;Pathogenesis;Pathway interactions;Patients;Peripheral Blood Mononuclear Cell;pharmacologic;Plasma;Play;Postoperative Period;prevent;Production;prophylactic;Prospective, cohort study;protein expression;Proteins;Public Health;research study;Risk;Risk Factors;Role;Sampling;Serum;Site;SLC11A2 gene;success;Testing;Therapeutic;therapeutic target;Time;Transferrin;translational immunology;uptake;Variant

PROJECT_TITLE: Hepcidin-Ferroportin-Iron Axis in Cardiac Surgery-associated Acute Kidney Injury

STUDY_SECTION: KNOD

STUDY_SECTION_NAME: Kidney, Nutrition, Obesity and Diabetes Study Section[KNOD]


NIH_SPENDING_CATS: Bioengineering;Genetics;Human Genome;Networking and Information Technology R&D (NITRD)

ORG_CITY: Chicago

ORG_NAME: UNIVERSITY OF ILLINOIS AT CHICAGO

ORG_STATE: IL

PHR: Program Director/Principal Investigator (Liang, Jie): Project Narrative We will develop computational models and tools to construct probability landscapes of networks of stochastically interacting proteins, DNA/RNAs, and other molecules, and to quantify the flow maps of fluxes and the exact topology of these landscapes, so their behavior and roles in influencing cellular phenotypes can be better understood. In addition, we will study the biophysics of 3D genome folding, and will develop computational tools based on polymer modeling to identify the minimal sets of genomic driver interactomes that dictate the 3D conformations of chromatin loci and identify those that persists across tissues and those that are formed adaptively in different cell types. OMB No. 0925-0001/0002 (Rev. 03/2020 Approved Through 02/28/2023) Page Continuation Format Page

PI_NAMEs: LIANG, JIE  (contact)

PROGRAM_OFFICER_NAME: NGUYEN, HAN

PROJECT_TERMS: 3-Dimensional;Algorithms;Behavior;Biological;biophysical analysis;Biophysics;Biopolymers;Cell Physiology;cell type;Cells;Chemicals;Chromatin;Chromatin Structure;Chromosomes;Complex;Computer Models;computerized tools;Data;Development;DNA;Elements;Embryonic Development;Enzymes;Epigenetic Process;Equation;Event;frontier;Genetic Transcription;Genome;genome-wide;genomic locus;Genomics;Grain;Heterogeneity;Hi-C;high dimensionality;improved;insight;Investigation;Kinetics;Maintenance;Maps;Messenger RNA;Methods;Modeling;Molecular;Molecular Conformation;network architecture;non-genetic;Nuclear;Nuclear Envelope;Phenotype;Polymers;Potassium Channel;preservation;Principal Investigator;Probability;Process;programs;Proteins;Reaction;RNA;RNA Splicing;Role;Sampling;scaffold;Signaling Molecule;stem cell differentiation;Structural Genes;Structure;three-dimensional modeling;Tissues;tool;transcription factor;Work

PROJECT_TITLE: Models and Algorithms for Biological Networks and Polymers: Stochastic Probability Landscape and Chromatin Ensembles


ADMINISTERING_IC: EB

IC_NAME: NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING

NIH_SPENDING_CATS: Bioengineering;Biotechnology;Genetics

PHR: Project Narrative There are more than 5,000 human diseases caused by known genetic variation, including mutations, insertions, and deletions, but programmable tools for gene insertion to reliably study and model these diseases are lacking. We propose a two-pronged approach to developing novel platforms for programmable insertion of large sequences (up to 10 kb) by (1) mining bacterial and archaeal systems to identify novel integrases that can be used in mammalian cells and (2) combining CRISPR gene editors and integrases to insert large payloads into genomes in a programmable and multiplexed manner that is independent of cellular repair pathways and can function in both dividing and non-dividing cells. We will apply these new technologies for both basic biological discovery, such as high-throughput protein interaction assays, as well as therapeutic uses, such as modification of T-cells for immune oncology, and will accelerate the study and modeling of disease biology, providing a chassis for further engineering of programmable gene insertion tools.

PI_NAMEs: ABUDAYYEH, OMAR O (contact);GOOTENBERG, JONATHAN SAMUEL

PROGRAM_OFFICER_NAME: DARINGER, NICHOLE MARIE

PROJECT_TERMS: Acceleration;Bacteriophages;Biochemical;Biodiversity;Bioinformatics;Biological;Biological Assay;Biological Process;biological research;Biology;Biomedical Research;Cell Line;Cell model;Cell Therapy;cell type;Cells;cellular engineering;Clustered Regularly Interspaced Short Palindromic Repeats;Complex;computational pipelines;Confocal Microscopy;CRISPR/Cas technology;cytokine;design;Development;Disease;disease model;DNA;DNA Repair;DNA Sequence Alteration;Electroporation;Elements;Engineering;Enzymes;epigenome;Formulation;Future;gene correction;Genes;Genetic Variation;Genome;genome editing;genome editor;Genome engineering;Guide RNA;human disease;Immune;Immunooncology;improved;in vitro Model;in vivo;innovation;insight;Integrase;Interphase Cell;lipid nanoparticle;Location;Mammalian Cell;Mediating;Methodology;Mining;Modality;Modification;Mutagenesis;Mutation;Neurons;new technology;novel;novel therapeutics;Pathway interactions;Positioning Attribute;postmitotic;prime editing;Protein Engineering;protein protein interaction;Proteins;Recording of previous events;recruit;Regulatory Element;repaired;Resources;response;RNA;screening;Site;stress granule;System;Systems Integration;T-Lymphocyte;Technology;Testing;Therapeutic;therapeutic development;Therapeutic Uses;tool;transcription factor;Virus;Visualization;Work

PROJECT_TITLE: Programmable gene integration and cell engineering with CRISPR-directed integrases

STUDY_SECTION: TAG

STUDY_SECTION_NAME: Therapeutic Approaches to Genetic Diseases Study Section[TAG]


ED_INST_TYPE: SCHOOLS OF PHARMACY

ORG_CITY: SAN FRANCISCO

ORG_NAME: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO

PHR: See Research Strategy Section for Project Narrative.

PI_NAMEs: FRASER, JAMES SOLOMON (contact)

PROGRAM_OFFICER_NAME: TAYLOR, VERONICA

PROJECT_TITLE: Equipment for Discovering and Manipulating Macromolecular Conformational Ensembles

STUDY_SECTION: MRAB

STUDY_SECTION_NAME: Maximizing Investigators’ Research Award B Study Section[MRAB]


NIH_SPENDING_CATS: Genetics;Stem Cell Research;Stem Cell Research - Embryonic - Non-Human

ORG_CITY: DAVIS

ORG_DEPT: SURGERY

ORG_NAME: UNIVERSITY OF CALIFORNIA AT DAVIS

PHR: EUCOMM Conditional Vector and ES Cell Clone Collection Acquisition and Procurement Plan by the MMRRC at UC Davis PROJECT NARRATIVE The Mutant Mouse Resource and Research Center (MMRRC) at UC Davis provides the research community with scientifically unique and valuable mouse resources to support biomedical studies into understanding human health and disease. A particularly important collection of conditional alleles from the EUCOMM project at Helmholtz Zentrum Munich are not readily available to the US research community, and are at risk of loss. Acquisition and procurement of this set of conditional vectors and alleles in ES cells will enhance the services provided by the MMRRC.

PI_NAMEs: LLOYD, KC KENT (contact)

PROGRAM_OFFICER_NAME: MIROCHNITCHENKO, OLEG

PROJECT_TERMS: Administrative Supplement;Alleles;Animal Model;Archives;Award;Biomedical Research;Budgets;California;Clone Cells;Clustered Regularly Interspaced Short Palindromic Repeats;Collection;Communities;cost;Data;data acquisition;Databases;Deposition;digital;Direct Costs;Disease;embryonic stem cell;Ensure;Equipment and supply inventories;ES Cell Line;falls;Funding;Future;Gene Targeting;Genes;Genetic;Goals;Grant;Health;Human;Human Biology;human disease;human model;interest;International;Knock-out;LacZ Genes;Logistics;Maintenance;Mediating;Metadata;migration;Mission;molecular vector;mouse model;Mus;Mutant Strains Mice;outreach;Ownership;parent project;Parents;Phase;Phenotype;Preparation;preservation;Process;Production;programs;Quality Control;Records;Research;Research Personnel;Resources;response;Risk;Sampling;Scanning;Security;Series;Services;Test Result;Testing;United States;United States National Institutes of Health;Universities;vector;Welding

PROJECT_TITLE: EUCOMM Conditional Vector and ES Cell Clone Collection (Year 24 Supplemental)


ADMINISTERING_IC: AA

IC_NAME: NATIONAL INSTITUTE ON ALCOHOL ABUSE AND ALCOHOLISM

NIH_SPENDING_CATS: Alcoholism, Alcohol Use and Health;Cancer;Chronic Liver Disease and Cirrhosis;Digestive Diseases;Liver Cancer;Liver Disease;Microbiome;Nutrition;Prevention;Rare Diseases;Substance Misuse

ORG_CITY: IRVINE

ORG_NAME: UNIVERSITY OF CALIFORNIA-IRVINE

PHR: Project Narrative Excessive consumption of fructose or alcohol causes fatty liver and occasionally liver cancer, but whether these two dietary risk factors synergistically induce liver cancer is unknown. In this proposal, we will determine how fructose intake alters liver handling of alcohol to increase liver cancer risk. To achieve this goal, we will employ state-of-the-art mass spectrometry and our newly generated genetically modified mice.

PI_NAMEs: JANG, CHOLSOON  (contact)

PROGRAM_OFFICER_NAME: MURRAY, GARY

PROJECT_TERMS: Acetate-CoA Ligase;Acetates;Acetyl Coenzyme A;Alcohol consumption;Alcoholism;Alcohols;Antibiotic Therapy;Antibiotics;Biochemical;Blood;cancer risk;cancer type;Carbon;carcinogenicity;Catabolism;Catalogs;Cause of Death;Charge;Chronic;Circulation;Cirrhosis;clinically relevant;colon microbiota;comparative;Consumption;Data;Development;diagnostic biomarker;dietary;dietary guidelines;drinking;Environment;Ethanol;Ethanol Metabolism;Event;Exposure to;Fatty acid glycerol esters;Fatty Liver;fatty liver disease;feeding;Feeds;Fructose;Gene Modified;Goals;gut microbiota;Hepatic;Histone Acetylation;in vivo;Incidence;Inflammatory;innovation;insight;Intake;knock-down;Leaky Gut;Liquid substance;Liver;liver inflammation;liver injury;Malignant neoplasm of liver;Malignant Neoplasms;Mass Spectrum Analysis;Measures;Mediating;Metabolic;Metabolism;metabolomics;Metagenomics;microbial;microbiota;microbiota metabolites;Molecular;mortality;mouse model;Mus;novel strategies;Oncogenic;Output;overexpression;Prevention strategy;Primary carcinoma of the liver cells;Production;Protocols documentation;Public Health;Risk;Risk Factors;soft drink;stable isotope;success;Techniques;Testing;tool;tumorigenic;Venous

PROJECT_TITLE: Elucidating dietary fructose and alcohol interactions during liver cancer development

STUDY_SECTION: HBPP

STUDY_SECTION_NAME: Hepatobiliary Pathophysiology Study Section[HBPP]


ORG_CITY: PASADENA

ORG_NAME: CALIFORNIA INSTITUTE OF TECHNOLOGY

PHR: Project narrative (Public health relevance statement) Mitochondria are indispensable organelles that generate energy and perform other vital functions for eukaryotic cells. Human health therefore depends on the proper function of mitochondria, and we are studying the processes that maintain mitochondrial function in cells. In the long-term, advances from these studies can be used to improve mitochondrial function and mitigate disease.

PI_NAMEs: CHAN, DAVID C (contact)

PROGRAM_OFFICER_NAME: ANSONG, CHARLES KWAKU

PROJECT_TERMS: Actins;Apoptosis;Astrocytes;Biogenesis;biological adaptation to stress;Calcium;Cell Physiology;Cells;Cellular biology;Cellular Stress;Central Nervous System;Chromatin Remodeling Factor;Citric Acid Cycle;Coupled;Coupling;CRISPR interference;Cytoskeleton;Data;density;Disease;Ensure;Equilibrium;Equipment;Eukaryotic Cell;Event;fatty acid metabolism;Future;Gene Expression;Generations;Genes;Goals;Health;Human;improved;in vivo;insight;Iron;Knowledge;Link;Lysosomes;Membrane Fusion;Metabolic;Mitochondria;Molecular;mouse genetics;Natural Immunity;Neurons;Oligodendroglia;Organelles;Oxidative Phosphorylation;Pathway interactions;Phenotype;Physiological;Physiology;Play;Population;Process;programs;public health relevance;Quality Control;Regulation;Research;Research Project Grants;response;Role;Signal Pathway;Signal Transduction;Sulfur;whole genome

PROJECT_TITLE: Homeostatic Mechanisms Regulating Mitochondrial Health  EQUIPMENT SUPPLEMENT


NIH_SPENDING_CATS: Genetics

ORG_CITY: TALLAHASSEE

ORG_DEPT: OTHER BASIC SCIENCES

ORG_NAME: FLORIDA STATE UNIVERSITY

ORG_STATE: FL

PHR: PROJECT NARRATIVE SUMOylation adds SUMO (a small polypeptide) to proteins, and polySUMOylation is a result of attachment of chains of SUMO. This project will investigate how polySUMOylation controls the relocation of key mitotic regulators during the cell cycle. Because deregulation of SUMOylation leads to genome instability and cancer development, the results will likely provide new strategies for the treatment of cancer and other diseases.

PI_NAMEs: WANG, YANCHANG  (contact)

PROGRAM_OFFICER_NAME: MILLER, RITA K

PROJECT_TERMS: Anaphase;Biochemistry;biological adaptation to stress;cancer diagnosis;cancer therapy;Cell Cycle;Cell Physiology;Cell Proliferation;Cells;Cellular biology;Chromosomes;Complex;Data;Development;Disease;DNA Damage;DNA Repair;Eukaryota;Genome Stability;Genomic Instability;Goals;Human;Knowledge;Lysine;Macromolecular Complexes;Malignant Neoplasms;Mitotic;Molecular;novel therapeutic intervention;Nucleolar Proteins;Phosphorylation;PLK1 gene;polypeptide;Post-Translational Protein Processing;protein complex;protein function;Proteins;Regulation;repaired;Research;Saccharomycetales;scaffold;Sumoylation Pathway;System;telophase;Testing;Translating;treatment strategy;Ubiquitin;Ubiquitination;Work;yeast genetics;Yeasts

PROJECT_TITLE: Temporal-spatial control of mitotic regulators by polySUMOylation

STUDY_SECTION_NAME: Cell Structure and Function 1 Study Section[CSF1]


NIH_SPENDING_CATS: Biotechnology;Genetics;Human Genome

ORG_CITY: SEATTLE

ORG_NAME: PACIFIC NORTHWEST RESEARCH INSTITUTE

PHR: PROJECT NARRATIVE Almost half of the human genome sequence has been created by self-replicating, genome-bound transposable elements like L1, and the replication of these elements can cause numerous diseases including cancer, autoimmunity, and infertility. Despite their persistence and prevalence, the functional consequences of the evolution of L1s is incompletely understood. We propose to study how L1s evade the factors used by the human genome to block their replication and how evasive L1s might impact human health. (3 sentences)

PI_NAMEs: MCLAUGHLIN, RICHARD NOEL (contact)

PROGRAM_OFFICER_NAME: MACARTHUR, LINDA

PROJECT_TERMS: Autoimmune Diseases;Autoimmunity;Automobile Driving;Basic Science;Binding;Biology;comparative genomics;Creativeness;Dedications;Defense Mechanisms;Directed Molecular Evolution;Disease;DNA;DNA Transposable Elements;Elements;Evolution;experience;experimental study;fascinate;Genetic;Genetic Diseases;Genome;genome analysis;Genomic Instability;Health;Human;Human Biology;Human Genome;In Vitro;Infertility;Innate Immune System;interest;Long Interspersed Elements;Malignant Neoplasms;Mentorship;Mutation;outreach;Pattern;pressure;Prevalence;prospective;Proteins;Recording of previous events;Repression;Research;Retroelements;Retrotransposon;RNA;Science;Scientist;Selfish DNA;Source;Speed;System;Variant

PROJECT_TITLE: Leveraging natural and directed evolution to dissect the functional consequences of sequence variation in human L1 retrotransposons

STUDY_SECTION_NAME: Special Emphasis Panel[ZRG1-CB-U(55)R]