Properties of Fcgamma receptors in normal and malignant human tissues.
Properties of the IgG receptors were studied by treating tissue sections or suspensions of peripheral mononuclear cells with various chemical reagents and determining changes in their ability to react with IgG-sensitized erythrocytes (EA). A heterogeneity of the Fc receptors was revealed. Iodoacetamide, 2-mercaptoethanol, sodium azide, EDTA, and a pH varying from 6.0 to 8.2 had no effect on Fc receptors in sections of normal lymphoreticular tissue and malignant tissue. Formaldehyde, low pH, and high salt concentrations affected receptor activity to various degrees. Receptors in liver sections and on monocytes were generally more resistant than receptors in spleen sections and on B lymphocytes. Receptors in malignant tissue behaved either like receptors in spleen or like receptors in liver. Although all tissues were sensitive to periodic acid, the Fc receptors in malignant tissue were always more resistant.
D001386:Azides / Q000494:pharmacology; D001666:Binding Sites, Antibody; D004492:Edetic Acid / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects / Q000276:immunology*; D005557:Formaldehyde / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007141:Immunoglobulin Fc Fragments / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007460:Iodoacetamide / Q000494:pharmacology; D008214:Lymphocytes / Q000276:immunology; D008623:Mercaptoethanol / Q000494:pharmacology; D009000:Monocytes / Q000276:immunology; D009369:Neoplasms / Q000276:immunology*; D010504:Periodic Acid / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D013154:Spleen / Q000276:immunology
D001386:Azides; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D010504:Periodic Acid; D005557:Formaldehyde; D012965:Sodium Chloride; D008623:Mercaptoethanol; D004492:Edetic Acid; D007460:Iodoacetamide