Protoplasts of Schizosaccharomyces pombe: an improved method for their preparation and the study of their guanine uptake.
A new method is described for the efficient conversion of Schizosaccharomyces pombe cells into protoplasts. The following parameters of guanine uptake determined in whole cells were unchanged in protoplasts: Km value, requirement for an energy source, sensitivity to competitive inhibitors, pH optimum, as well as the typical variation of the initial velocity of uptake observed during the growth phase.
D000225:Adenine / Q000494:pharmacology; D001203:Ascomycota / Q000378:metabolism*; D001375:Azaguanine / Q000494:pharmacology; D001386:Azides / Q000494:pharmacology; D002473:Cell Wall / Q000378:metabolism; D003470:Culture Media; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006128:Growth; D006147:Guanine / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007042:Hypoxanthines / Q000494:pharmacology; D011134:Polysaccharides; D011523:Protoplasts / Q000378:metabolism* / Q000502:physiology; D012440:Saccharomyces / Q000254:growth & development; D012568:Schizosaccharomyces / Q000378:metabolism*; D012908:Snails / Q000201:enzymology
D001386:Azides; D003470:Culture Media; D007042:Hypoxanthines; D011134:Polysaccharides; D006147:Guanine; D006026:Glycoside Hydrolases; D000225:Adenine; D001375:Azaguanine