Introduction of artificial crosslinks into proteins.
In this chapter, we present a brief overview of the current status of protein crosslinking technology. An attempt is made to compare the natural crosslinks and natural crosslinking agents to the artificial ones, and a brief section is devoted to the potential use of enzymes (transglutaminase and peroxidase) as crosslinking agents in vitro. Homobifunctional (x-R-x) and heterobifunctional (x-R-y) reagents are considered in terms of the kinds of functional groups and R-groups that have been used in protein crosslinking, and some examples of reagents and applications from the recent literature are tabulated.
D055598:Chemical Phenomena; D002621:Chemistry; D004220:Disulfides; D004912:Erythrocytes / Q000201:enzymology; D006801:Humans; D007202:Indicators and Reagents; D008722:Methods; D011485:Protein Binding; D011506:Proteins; D013329:Structure-Activity Relationship; D005723:gamma-Glutamyltransferase / Q000302:isolation & purification / Q000378:metabolism
D004220:Disulfides; D007202:Indicators and Reagents; D011506:Proteins; D005723:gamma-Glutamyltransferase