Characterization of the metabolites of ellipticine in rat bile.
The two major metabolites of ellipticine (NSC 71795) were isolated from rat bile by a combination of solvent extraction, partition column chromatography, and reverse phase high-performance liquid chromatography. Purification and structural elucidation of the bile products were aided by administration of the drug with a dual label (14C and 2H). The two metabolites were shown to be the sulfate and glucuronide conjugates of 9-hydroxyellipticine by chemical, enzymatic, and mass-spectral fragmentation comparison with synthetic and enzymatically prepared reference compounds.
D000470:Alkaloids / Q000378:metabolism*; D000818:Animals; D001646:Bile / Q000378:metabolism*; D055598:Chemical Phenomena; D002621:Chemistry; D002845:Chromatography; D004611:Ellipticines / Q000378:metabolism*; D005965:Glucuronates / Q000378:metabolism; D006900:Hydroxylation; D008297:Male; D051381:Rats; D013057:Spectrum Analysis; D013431:Sulfates / Q000378:metabolism
D000470:Alkaloids; D004611:Ellipticines; D005965:Glucuronates; D013431:Sulfates