Complement activating factor(s) of Trypanosoma lewisi: some physiochemical characteristics of the active components.
Of the complement activating factors present in Trypanosoma lewisi, the major component, a carbohydrate containing substance was further investigated. This component was found to have a lag time of complete activation of 2 CH50 units of bovine complement of approximately 15 minutes while 1% trypsin (a known activator of complement, used as a control system) was capable of instant consumption of a similar quantity of complement. In addition, the complement activating factor of trypanosomes was observed to be stable at 100 degrees C for 15 minutes and over a pH range of 3.0 to 11.0. Thin layer chromatography studies suggested that at least part of the active component contained lipid, perhaps indicating that it may be glycolipid in nature.
D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D003165:Complement System Proteins / Q000032:analysis / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D013696:Temperature; D014350:Trypanosoma lewisi / Q000032:analysis / Q000276:immunology*
D002241:Carbohydrates; D003165:Complement System Proteins