An assay of ribonuclease H, endoribonucleases, and phosphatases.
An assay for ribonuclease H (EC 3.1.4.34) is described which permits the estimation of the number and the type of cleavages produced by the enzyme when acting on the ribo moiety of a DNA-RNA hybrid substrate. With six different homopolymer hybrids tested, the number of enzymically induced breaks varied widely. The method is applicable to other endoribonucleases, phosphodiesterases, and phosphatases.
D004720:Endonucleases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009843:Oligoribonucleotides / Q000032:analysis; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D012260:Ribonucleases / Q000378:metabolism*; D012995:Solubility; D012997:Solvents
D009843:Oligoribonucleotides; D012997:Solvents; D004720:Endonucleases; D012260:Ribonucleases; D010744:Phosphoric Monoester Hydrolases