[Hydrolysis of insoluble collagen of bull bones by Streptomyces griseus crystalline protease].
Hydrolysis of collagen was studied in the bull bone tissues by the Str. griseus crystalline protease. The amount of collagen hydrolyzed by it composed 6.6% and 16% after 4-hour and 6-hour hydrolysis, respectively. When the enzyme:substrate ratio is 1:50 hydrolysis proceeds most intensively; with a decrease in the ratio up to 1:1000 the average amount of peptides increase from 2.6 up to 4 amino acidic residua, respectively. Under conditions of denaturated collagen hydrolysis the content of hydroxyproline in solution as compared with the native one increases; in this case the links with the presence of imino-acids are easier to split, the more resistant being those formed by hydroxyproline. Within the limit of 20-45 degrees C hydrolysis of protein intensifies with a temperature rise. Within the pH range of 5.0-11.0 the maximal amount of alpha- NH2-groups and hydroxyproline is observed at pH 8.5, the minimal--at PH 5.0. Hydroxyproline in the composition of peptides appears at the beginning of hydrolysis whereas the free one of enzymes of the longer effect 24 h after the beginning of the experiment composes 12.2% of its total content in the solved products. In the insoluble part of the substrate after 3-hour hydrolysis tyrosine composes less than 25% of its initial amount in protein whereas phenyl alanine--over 70%. After 6-hour hydrolysis the solved part of the system contains about 30% of alanine and 8.9 and 6% of glycine, proline and hydroxyproline, respectively.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001842:Bone and Bones / Q000032:analysis*; D002417:Cattle; D003094:Collagen / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007700:Kinetics; D008297:Male; D010447:Peptide Hydrolases / Q000378:metabolism; D013305:Streptomyces griseus / Q000201:enzymology
D000596:Amino Acids; D003094:Collagen; D010447:Peptide Hydrolases
Gidroliz nerozchinnogo kolagenu kistok bika Kristalichnoiu proteazoiu Streptomyces griseus