Two glycosulfatases from the liver of a marine gastropod, Charonia lampas. Partial purification and properties.
Two glycosulfatases [EC 3.1.6.3], I and II, were purified 31.3- and 33.9-fold respectively, from a crude extract of the liver of Charonia lampas. The purification was carried out by the following chromatographic procedures; phosphocellulose, Sephadex G-150, Concanavalin A-Sepharose and isoelectric focussing. The enzyme preparations obtained were practically free from arylsulfatase [EC 3.1.6.1] contamination. Both glycosulfatases are probably glycoproteins differing in their carbohydrate moieties. The molecular weights of glycosulfatase I and II were estimated to be about 112,000 and 79,000 respectively. They had the same optimum pH of 5.5, and the same Km value of 25.0 mM for glucose 6-sulfate.
D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D037102:Lectins; D008099:Liver / Q000201:enzymology*; D008970:Molecular Weight; D008974:Mollusca / Q000201:enzymology*; D010710:Phosphates / Q000494:pharmacology; D013429:Sulfatases / Q000302:isolation & purification / Q000378:metabolism*
D037102:Lectins; D010710:Phosphates; D013429:Sulfatases; D005947:Glucose