Regulation of pyruvate dehydrogenase and pyruvate dehydrogenase phosphate phosphatase activity in rat epididymal fat-pads. Effects of starvation, alloxan-diabetes and high-fat diet.
1. Pyruvate dehydrogenase phosphate phosphatase activity in rat epididymal fat-pads was measured by using pig heart pyruvate dehydrogenase [32P]phosphate. About 80% was found to be extramitochondrial and therefore probably not directly concerned with the regulation of pyruvate dehydrogenase activity. The extramitochondrial activity was sensitive to activation by Ca2+, but perhaps less sensitive than the mitochondrial activity.
D000273:Adipose Tissue / Q000187:drug effects / Q000201:enzymology*; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism; D004041:Dietary Fats; D004822:Epididymis / Q000201:enzymology; D005947:Glucose / Q000378:metabolism; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology; D008291:Malate Dehydrogenase / Q000378:metabolism; D008297:Male; D009249:NADP; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D011769:Pyruvate Dehydrogenase (Lipoamide)-Phosphatase / Q000378:metabolism*; D011768:Pyruvate Dehydrogenase Complex / Q000378:metabolism*; D051381:Rats; D013217:Starvation; D013997:Time Factors
D004041:Dietary Fats; D007328:Insulin; D011768:Pyruvate Dehydrogenase Complex; D009249:NADP; D008291:Malate Dehydrogenase; D010744:Phosphoric Monoester Hydrolases; D011769:Pyruvate Dehydrogenase (Lipoamide)-Phosphatase; D005947:Glucose; D002118:Calcium