The stereochemical course of acetate activation by yeast acetyl-CoA synthetase.
The purified alpha-thiophosphate diastereoisomers of adenosine 5'-(1-thio)-triphosphate were used to study the stereochemical course of the reaction catalyzed by yeast acetyl-CoA synthetase. Asymmetrically labeled adenosine 5'-thiophosphate was formed from the "B" diastereoisomer of adenosine 5'-(1-thio)-triphosphate and [18O]acetate. The label was found to be in the opposite orientation from the leaving pyrophosphate group showing that the acetate activation step occurred with inversion of configuration at the alpha-phosphorus.
D000106:Acetate-CoA Ligase / Q000378:metabolism*; D000085:Acetates / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000031:analogs & derivatives; D003066:Coenzyme A Ligases / Q000378:metabolism*; D004789:Enzyme Activation; D010103:Oxygen Isotopes; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D013237:Stereoisomerism; D013873:Thionucleotides
D000085:Acetates; D010103:Oxygen Isotopes; D013873:Thionucleotides; D000255:Adenosine Triphosphate; D003066:Coenzyme A Ligases; D000106:Acetate-CoA Ligase