Determination of blood ionized calcium in a large segment of the normal adult population.
In two different laboratories ionized calcium was determined by use of a calcium selective electrode system of recent design in specimens of whole blood drawn from a total of 100 volunteers. Identical mean values were obtained in each laboratory. A small standard deviation was found supporting the view that [Ca2+] is normally maintained within a narrow range. Ancillary factors in [Ca2+] determination were evaluated, including effects of in-vivo produced changes in pH, and effects of addition of small amounts of heparin to the whole blood sample. A veno-arterial difference in [Ca2+] was insignificant.
D000293:Adolescent; D000328:Adult; D001158:Arteries; D002118:Calcium / Q000097:blood*; D002413:Cations, Divalent; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008875:Middle Aged; D010949:Plasma / Q000032:analysis; D012016:Reference Values; D014680:Veins
D002413:Cations, Divalent; D006493:Heparin; D002118:Calcium