Restriction and modification in B. subtilis. Purification and general properties of a restriction endonuclease from strain R.
All Bacillus subtilis R-type strains showing the phenomena of restriction and modification contain an endonuclease that inactivates in vitro the biological activity of a variety of DNAs lacking R-specific modification, such as transfecting SPPI, SPO2 and phi105 DNA, and transforming B. subtilis 168-type DNA. The corresponding DNAs carrying R-specific modification are resistant to the enzyme. The enzyme has been purified approximately 400-fold and is essentially free from contaminating double strand-directed unspecific exo- or endonuclease activity. Only Mg2+ is required as cofactor. The substrate DNAs are cleaved at specific sites. The double-stranded fragments produced from SPP1 DNA (molecular weight 2.5 x 10(7)) have an average molecular weight of about 3 x 10(5).
D000255:Adenosine Triphosphate / Q000494:pharmacology; D001412:Bacillus subtilis / Q000201:enzymology*; D001435:Bacteriophages; D002118:Calcium / Q000494:pharmacology; D003090:Coliphages; D004262:DNA Restriction Enzymes / Q000302:isolation & purification* / Q000378:metabolism; D004269:DNA, Bacterial / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism; D004720:Endonucleases / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D010641:Phenotype; D012436:S-Adenosylmethionine / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D013045:Species Specificity; D014170:Transformation, Genetic
D004269:DNA, Bacterial; D004279:DNA, Viral; D008345:Manganese; D012965:Sodium Chloride; D012436:S-Adenosylmethionine; D000255:Adenosine Triphosphate; D004720:Endonucleases; D004262:DNA Restriction Enzymes; D008274:Magnesium; D002118:Calcium