[Structural lability of biomembranes and their components studied by fluorescent analysis. V. Liposomes].
The nature of binding of ionic and neutral fluorescent probes in liposome structures of natural lecithin at various pH and ionic strength of the medium. Temperature relationship of fluorescence intensity of both probes presented in Arrhenius coordinates is shown to have bends at 32--42degrees C. A smaller bend in the temperature range of 20--40degrees C is fixed while studying the correlation time of the spin probe, line width of (CH2)n groups in the NMR-spectrum and light scattering at the angle of 90 degrees. Differences between the sensitivity of these procedures to the phase transition in the lipid structure and the method of measuring fluorescence probe intensity is discussed in terms of literature data.
D006863:Hydrogen-Ion Concentration; D008081:Liposomes; D009682:Magnetic Resonance Spectroscopy; D009994:Osmolar Concentration; D010713:Phosphatidylcholines; D013696:Temperature
D008081:Liposomes; D010713:Phosphatidylcholines
Issledovanie strukturnoĭ labil'nosti biomembran i ikh komponentov metodom fluorestsentnogo analiza. V. Liposomy