Purification and some properties of Bacillus macerans cycloamylose (cyclodextrin) glucanotransferase.
Bacillus macerans cycloamylose (cyclodextrin) glucanotransferase (EC 2.4.1.19) was purified by the technique of starch adsorption and DEAE-cellulose column chromatography, and then crystallized from an ammonium sulfate solution containing mM calcium chloride. The crystals of the enzyme were rod-shaped and showed a single band by disc-gel electrophorsis. The purified enzyme was dissociated into two subunits by sodium dodecyl sulfate-disc electrophoresis. The subunits had no enzyme activity. Details of each purification step and some properties of the enzyme are described in this paper.
D001407:Bacillus / Q000201:enzymology*; D003460:Crystallization; D004355:Drug Stability; D005964:Glucosyltransferases / Q000302:isolation & purification* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008722:Methods; D008970:Molecular Weight; D013696:Temperature
D005964:Glucosyltransferases