Preparative separation of the complementary strands of DNA restriction fragments by alkaline RPC-5 chromatography.
High pressure reversed phase chromatography (RPC-5) at pH 12 was used for preparative separation of the complementary strands of the smaller DNA fragments which are generated by the Hae III restriction endonuclease digestion of Col El DNA. A single high pressure RPC-5 chromatographic step at neutral pH served to purify duplex fragments 70, 172, 250 and 440 base pairs long; each of these yielded two elution peaks upon chromatography under alkaline denaturing conditions.
D003086:Bacteriocin Plasmids; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004277:DNA, Single-Stranded / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight
D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D004262:DNA Restriction Enzymes