Type I Escherichia coli pili: characterization of binding to monkey kidney cells.
We have demonstrated binding of purified pili from a strain of Escherichia coli to Vero cell monolayers as a model of prokaryotic-eukaryotic cell adherence. Pili bound to the tissue culture in a rapid reaction that did not require enzymatic activation. Attachment occurred optimally at pH 4-5 and could be inhibited by analogues of D-mannose, anti-pili antibodies, or by preincubation of tissue cells with mannose-specific plant lectins. Binding remained after treatment of the monolayer with glycosidases, trypsin, or a protease mixture but was enhanced after neuraminidase treatment. These results indicate that bacterial binding can occur via pili which act like lectins and presumably bind to mannose-containing glycoproteins on mammalian cell surfaces.
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins / Q000378:metabolism*; D001665:Binding Sites; D002473:Cell Wall / Q000276:immunology / Q000378:metabolism; D002478:Cells, Cultured; D004798:Enzymes / Q000494:pharmacology; D004926:Escherichia coli / Q000276:immunology / Q000378:metabolism* / Q000648:ultrastructure; D006241:Haptens; D006863:Hydrogen-Ion Concentration; D037102:Lectins / Q000494:pharmacology; D011485:Protein Binding / Q000187:drug effects; D013696:Temperature
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D004798:Enzymes; D006241:Haptens; D037102:Lectins