Lipid metabolism by The gall-bladder. II. The in vitro conversion of lysophosphatidylcholine to phosphatidylcholine.
Lysophosphatidylcholine acyltransferase, which catalyzes the acylation of lysophosphatidylcholine with fatty acid coenzyme A to form phosphatidylcholine, was assayed in gall-bladder mucosa. In guinea pig gall-bladder the activity parallels that of the microsomal enzyme, glucose-6-phosphatase with 3--4-fold enrichment of the activity in the microsomes. Studies with saturated and unsaturated substrates demonstrated highest activity when oleoyl coenzyme A and palmitoyl lysophosphatidylcholine were used and the lowest activity when palmitoyl coenzyme A and palmitoyl lysophosphatidylcholine were used. This activity was demonstrated in the dog, rabbit, cat, calf and human gall-bladder mucosa; however, a wide variation in the amount was observed. Lysophospholipase, which catalyzes the hydrolysis of lysophosphatidylcholine to glycerophosphorylcholine and fatty acid, was also demonstrated in gall-bladder mucosa.
D008243:1-Acylglycerophosphocholine O-Acyltransferase / Q000378:metabolism*; D000214:Acyl Coenzyme A; D000217:Acyltransferases / Q000378:metabolism*; D000818:Animals; D002415:Cats; D002417:Cattle; D004285:Dogs; D005704:Gallbladder / Q000201:enzymology*; D006168:Guinea Pigs; D006801:Humans; D009829:Oleic Acids; D010171:Palmitoyl Coenzyme A; D010170:Palmitoyl-CoA Hydrolase / Q000378:metabolism; D011817:Rabbits
D000214:Acyl Coenzyme A; D009829:Oleic Acids; D010171:Palmitoyl Coenzyme A; D000217:Acyltransferases; D008243:1-Acylglycerophosphocholine O-Acyltransferase; D010170:Palmitoyl-CoA Hydrolase