Isolation and characterization of isoenzymes of human salivary and pancreatic alpha-amylase.
Human salivary and pancreatic alpha-amylase (1,4-glucan 4-glucanohydrolase, EC 3.2.1.1) were separated by electrofocusing. In the first case we obtained six isoenzymes with isoelectric points of pH 5.70, 5.72, 6.23, 6.32, 6.73 and 6.88. Human pancreatic alpha-amylase has been separated into eight isoenzymes with isoelectric points of pH 5.72, 5.77, 5.88, 6.05, 6.23, 6.69, 6.72 and 6.95. Some of the isoenzymes were shown to be sialoproteins; others representing about 80% of the total activity did not contain neuraminic acid. The molecular weight of the non-sialoproteinic isoenzymes was found to be about 47 000 in all cases.
D000681:Amylases / Q000302:isolation & purification*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000302:isolation & purification; D008970:Molecular Weight; D009439:Neuraminidase / Q000378:metabolism; D010179:Pancreas / Q000201:enzymology*; D012469:Salivary Glands / Q000201:enzymology*
D007527:Isoenzymes; D000681:Amylases; D009439:Neuraminidase