beta-Adrenergic receptors in rat kidney: direct localization by a fluorescent beta-blocker.
A new fluorescent beta-adrenergic blocker, 9-amino-acridin propranolol (9-AAP), was administered intravenously to albino rats. Fluorescent aggregates of 9-AAP binding sites were concentrated in the vascular poles of the glomeruli in association with the preglomerular afferent arterioles. 9-AAP fluorescence was reduced following pretreatment with (+/-)- and (-)-propranolol and was less affected in rats pretreated by the (+)-racemic isomer. 9-AAP binding sites were also observed on the lining epithelium of the renal collecting system. Our data suggest that 9-AAP may label beta-adrenergic receptors in rat kidney.
D000319:Adrenergic beta-Antagonists; D000818:Animals; D005260:Female; D007275:Injections, Intravenous; D007606:Juxtaglomerular Apparatus / Q000648:ultrastructure; D007668:Kidney / Q000648:ultrastructure*; D007672:Kidney Cortex / Q000648:ultrastructure; D007684:Kidney Tubules / Q000648:ultrastructure; D008297:Male; D011433:Propranolol / Q000031:analogs & derivatives*; D051381:Rats; D011941:Receptors, Adrenergic; D011943:Receptors, Adrenergic, beta; D013237:Stereoisomerism
D000319:Adrenergic beta-Antagonists; D011941:Receptors, Adrenergic; D011943:Receptors, Adrenergic, beta; D011433:Propranolol