Pyrrolidone carboxylyl peptidase in Streptococcus cremoris: dependence on an interaction with membrane components.
A study of the distribution of pyrrolidone carboxylyl peptidase (PCP) activity among cell fractions of Streptococcus cremoris HP revealed that this enzyme is associated with a particulate fraction, which mainly consists of membrane material. This location could only be established using a gentle nonmechanical method for the disruption of spheroplasts under the conditions of which intracellular marker enzymes are released. The effect of monovalent anions and treatments, which do not destroy covalent binding, suggests an association of the enzyme with surrounding structures determined by both hydrophobic and electrostatic interactions. The activity of PCP associated with cells harvested from different growth phases and in the solubilized state was studied as a function of the temperature in the absence and in the presence of the membrane-interfering agent n-butanol. A decrease in the apparent activation energy, inherent to the solubilized enzyme, is induced in situ at a lower transition temperature. Only with logarithmic-phase cells is this transition followed (mid-logarithmic cells) or accompanied (late logarithmic cells) by a secondary decrease in the energy of activation. n-Butanol appeared to decrease the lower transition temperature of the enzyme activity in situ, and additionally it exerted an effect on the manifestation of the secondary transition. Thecorganization of membrane components, mainly the lipids.
D000626:Aminopeptidases / Q000378:metabolism*; D001965:Bromides / Q000494:pharmacology; D000440:Butanols / Q000494:pharmacology; D003486:Cyanides / Q000494:pharmacology; D005459:Fluorides / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007454:Iodides / Q000494:pharmacology; D008566:Membranes; D011188:Potassium / Q000494:pharmacology; D011751:Pyroglutamyl-Peptidase I / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology; D013291:Streptococcus / Q000201:enzymology* / Q000254:growth & development; D013347:Subcellular Fractions / Q000201:enzymology; D013696:Temperature
D001965:Bromides; D000440:Butanols; D003486:Cyanides; D007454:Iodides; D012965:Sodium Chloride; D000626:Aminopeptidases; D011751:Pyroglutamyl-Peptidase I; D005459:Fluorides; D011188:Potassium