Analysis of medazepam, diazepam, and metabolites in plasma by gas-liquid chromatography with electrolytic conductivity detection.
An improved electrolytic conductivity detector allowed the gas-liquid chromatographic analysis of medazepam, diazepam, and major metabolites in 2 ml plasma at concentrations of 20 microgram/l. The detector had a sensitivity limit of less than 1 ng (or 100 pg nitrogen) when operated in the nitrogen-selective mode and a nitrogen/carbon elemental selectivity ratio of greater than 100,000 compared to octadecane and cholesterol. Detector response when operated in various element-selective chemical modes was investigated, and its application to the analysis of the title compounds was compared to electron capture and flame ionization detection systems.
D014151:Anti-Anxiety Agents / Q000097:blood*; D002849:Chromatography, Gas / Q000379:methods; D002853:Chromatography, Liquid; D003975:Diazepam / Q000097:blood*; D004553:Electric Conductivity; D008472:Medazepam / Q000031:analogs & derivatives / Q000097:blood*; D008832:Microchemistry; D003708:Nordazepam / Q000097:blood
D014151:Anti-Anxiety Agents; D003708:Nordazepam; D008472:Medazepam; D003975:Diazepam