Nonenzymatic reactivation of des-acetyl citrate lyase by acetyl adenylate. First example of enzyme activation by chemotrophic modification.
The experimental conditions of nonenzymatic reactivation of des-acetyl citrate lyase from K. aerogenes were studied. It was found that at pH 8.5 0.2 MM acetyl AMP causes a fast reactivation of the enzyme. The pH dependence of activity and the Km for citrate are very similar for both native and reactivated enzyme.
D001275:ATP Citrate (pro-S)-Lyase / Q000378:metabolism*; D000249:Adenosine Monophosphate / Q000031:analogs & derivatives* / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007709:Klebsiella / Q000201:enzymology; D007652:Oxo-Acid-Lyases / Q000378:metabolism*
D000249:Adenosine Monophosphate; D001275:ATP Citrate (pro-S)-Lyase; D007652:Oxo-Acid-Lyases