[Effect of pH on bacteria in early log phase].
The bacteria harvested in the early log phase lyse when they are submitted to a pH above 10. The peptidoglycan is not degraded in these conditions. Thus, the authors used these properties to extract the peptidoglycan from several gram negative and gram positive bacteria.
D001410:Bacillus megaterium / Q000254:growth & development; D001433:Bacteriolysis; D004755:Enterobacteriaceae / Q000032:analysis* / Q000254:growth & development; D004926:Escherichia coli / Q000032:analysis; D006020:Glycopeptides / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D009016:Moraxella / Q000032:analysis; D009343:Neisseria / Q000032:analysis; D009346:Neisseriaceae / Q000032:analysis* / Q000254:growth & development; D011511:Proteus / Q000032:analysis / Q000648:ultrastructure
D006020:Glycopeptides
Influence du pH sur des bactéries en phase de croissance