Identification of the chemical groups involved in the binding of periodate-oxidized NADP+ to 6-phosphogluconate dehydrogenase.
Periodate-oxidized NADP+ binds specifically and reversibly to the NADP+ binding site of 6-phosphogluconate dehydrogenase (EC 1.1.1.44) from Candida utilis. The inhibition can be stabilized by reduction with sodium borohydride. It has been shown that an aldehydic group of the inhibitor forms a Schiff base with a lysine residue of the enzyme.
D001665:Binding Sites; D002175:Candida / Q000201:enzymology; D008239:Lysine; D009249:NADP / Q000494:pharmacology*; D010504:Periodic Acid; D010734:Phosphogluconate Dehydrogenase / Q000037:antagonists & inhibitors*; D011485:Protein Binding
D010504:Periodic Acid; D009249:NADP; D010734:Phosphogluconate Dehydrogenase; D008239:Lysine