Histone-like protein fractions of Kluyveromyces fragilis and their relation to the cell cycle.
Protein fractions were obtained from Kluyveromyces fragilis by pH titration, a technique used for histone extraction, following the inclusion of a distilled water extraction stage, and the fractions partially characterized. The inclusion of a distilled water step resulted in a tenfold purification of the fraction obtained at pH 2.20 as compared with pH titration alone. In synchronous cultures induced with 2'-deoxyadenosine or prepared by selection this fraction displayed a stepwise accumulation, and doubled in quantity at a point about one third of a cycle after cell division. The fraction obtained at pH 1.35 resembled calf thymus f2a2 histone in its extraction properties and amino acid composition, and also showed a possible stepwise accumulation. Other fractions appear to accumulate exponentially.
D000596:Amino Acids / Q000032:analysis; D001203:Ascomycota / Q000032:analysis*; D002455:Cell Division / Q000187:drug effects; D003839:Deoxyadenosines / Q000494:pharmacology; D005656:Fungal Proteins / Q000032:analysis* / Q000302:isolation & purification; D006657:Histones / Q000032:analysis* / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D008722:Methods; D004718:Saccharomycetales / Q000032:analysis* / Q000254:growth & development
D000596:Amino Acids; D003839:Deoxyadenosines; D005656:Fungal Proteins; D006657:Histones