Isolation and characterization of two sequence-specific endonucleases from Anabaena variabilis.
Two endonucleases, AvaI and AvaII, were isolated from Anabaena variabilis on the basis of their ability to make a limited number of breaks at specific points in bacteriophage lambda DNA. Neither enzyme has cofactor requirements beyond Mg2+. Endonuclease AvaI makes eight breaks in the phage lambda chromosome at which the 5'-terminal sequence is pPy-C-G-N. AvaII endonuclease cuts phage lambda DNA more extensively, yielding fragments with the 5'-terminal sequence G-T-C-N or G-A-C-N. Neither enzyme generates cohesive ends.
D001419:Bacteria / Q000201:enzymology*; D001483:Base Sequence; D003090:Coliphages; D004279:DNA, Viral / Q000378:metabolism; D003851:Deoxyribonucleases; D004587:Electrophoresis, Agar Gel; D004720:Endonucleases / Q000302:isolation & purification* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008274:Magnesium; D010744:Phosphoric Monoester Hydrolases
D004279:DNA, Viral; D003851:Deoxyribonucleases; D004720:Endonucleases; D010744:Phosphoric Monoester Hydrolases; D008274:Magnesium