Contrast manifestation of alkaline phosphatase and 5'-nucleotidase in plasma membranes isolated from rat liver and ascites hepatoma.
1. Plasma membranes were isolated from ascites hepatoma AH-130 and rat livers with or without partial hepatectomy or bile duct ligation. Reciprocal manifestations of two marker enzymes for plasma membranes were observed in these membrane preparations; alkaline phosphatase activity was found much higher in the hepatoma membrane than in any preparations of the liver membranes, whereas 5'-nucleotidase activity was much lower in the former than in the latter. 2. Effects of lectins and anti-plasma membrane antiserum on these two marker enzymes were examined. The results showed that about 50% of apparent activity of 5'-nucleotidase found in the hepatoma membrane was exhibited by alkaline phosphatase. 3. Localizations of alkaline phosphatase and 5'-nucleotidase in polyacrylamide gels after electrophoresis were demonstrated using 5'-AMP and 5-Br, 4-Cl-indoxylphosphate as substrate. There was a difference in electrophoretic mobility between the alkaline phosphatase of the hepatoma and that of the liver.
D000249:Adenosine Monophosphate / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D000906:Antibodies; D006528:Carcinoma, Hepatocellular / Q000201:enzymology*; D002462:Cell Membrane / Q000201:enzymology / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D006639:Histidine / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D037102:Lectins / Q000494:pharmacology; D008099:Liver / Q000201:enzymology*; D008113:Liver Neoplasms / Q000201:enzymology*; D008297:Male; D009374:Neoplasms, Experimental / Q000201:enzymology; D009708:Nucleotidases / Q000276:immunology / Q000378:metabolism*; D051381:Rats
D000906:Antibodies; D037102:Lectins; D000249:Adenosine Monophosphate; D006639:Histidine; D009708:Nucleotidases; D000469:Alkaline Phosphatase