[Glutamine synthetase immobilization].
Highly purified glutamine synthetase has been isolated from Chlorella and immobilized on BrCN-sepharose. Its residual activity was 25-35%. Immobilized glutamine synthetase showed far greater thermal stability than glutamine synthetase in solution. During immobilization pH optimum of the enzyme was shifted towards the alkaline area, maximum rate of the reaction was reduced and KM remained unaltered.
D002708:Chlorella / Q000201:enzymology; D004355:Drug Stability; D004789:Enzyme Activation; D004800:Enzymes, Immobilized; D005974:Glutamate-Ammonia Ligase / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D013696:Temperature
D004800:Enzymes, Immobilized; D005974:Glutamate-Ammonia Ligase
Immobilizatsiia glutaminsintetazy.