Isolation and identification of the major metabolite of albuterol in human urine.
The major urinary metabolite of albuterol in man was isolated and purified by ion-exchange chromatography and countercurrent distribution. On the basis of proton magnetic resonance, infrared, and mass-spectral analysis, enzymic hydrolysis, and assay of inorganic sulfate after acid hydrolysis, the metabolite has been identified as the 4'-O-sulfate ester of albuterol.
D000420:Albuterol / Q000652:urine*; D001192:Arylsulfatases; D002852:Chromatography, Ion Exchange; D003377:Countercurrent Distribution; D005966:Glucuronidase; D006801:Humans; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D013058:Mass Spectrometry; D013055:Spectrophotometry, Infrared; D013431:Sulfates / Q000652:urine
D013431:Sulfates; D001192:Arylsulfatases; D005966:Glucuronidase; D000420:Albuterol