Isolation from bovine brain of a fraction containing capillaries and a fraction containing membrane fragments of the choroid plexus.
Combined differential and density gradient centrifugation was used for the isolation of a capillary-rich fraction from the cerebral cortex and a brush border containing fraction from the bovine choroid plexus. The activities of gamma-glutamyl transpeptidase and several other marker enzymes were monitored during the fractionation procedure. Electron microscopic examination showed a membrane-rich fraction in the choroid plexus high in the gamma-glutamyl transpeptidase and 5'-nucleotidase activities. From the brain cortex, a capillary-rich fraction was obtained which was high in gamma-glutamyl transpeptidase and alkaline phosphatase activities. A histochemical examination showed gamma-glutamyl transpeptidase activity localized in the capillary walls.
D000135:Acid Phosphatase / Q000032:analysis; D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D002196:Capillaries / Q000201:enzymology*; D002417:Cattle; D002458:Cell Fractionation; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D002540:Cerebral Cortex / Q000098:blood supply*; D002831:Choroid Plexus / Q000201:enzymology*; D006651:Histocytochemistry; D008566:Membranes / Q000648:ultrastructure; D009708:Nucleotidases / Q000032:analysis; D005723:gamma-Glutamyltransferase / Q000032:analysis
D005723:gamma-Glutamyltransferase; D009708:Nucleotidases; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase