Overmethylation of DNAs by the EcoRI methylase.
EcoRI methylase is able to catalyze methy incorporation into DNA at sequences other than the canonical EcoRI site. At high enzyme concentrations and over a wide range of pH and ionic strengths, EcoRI methylase modifies polyoma DNA (which contains one EcoRI site) at a number of sites. This modification prevents EcoRI endonuclease activity, and thus is presumably at or near the EcoRI sequences (5') NAATTN.
D000225:Adenine / Q000378:metabolism; D001483:Base Sequence; D001973:Bromodeoxyuridine / Q000378:metabolism; D003090:Coliphages; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008780:Methyltransferases / Q000378:metabolism*; D009994:Osmolar Concentration; D011120:Polyomavirus / Q000235:genetics; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); D013379:Substrate Specificity
D004279:DNA, Viral; C020098:DNA modification methylase EcoRI; D008780:Methyltransferases; D015265:Site-Specific DNA-Methyltransferase (Adenine-Specific); D004262:DNA Restriction Enzymes; D001973:Bromodeoxyuridine; D000225:Adenine