Purification and properties of an induced beta-D-glucosidase from stachybotrys atra.
We have purified an induced beta-D-glucosidase (beta-D-glucoside glucohydrolase, EC 3.2.1.21) from Stachybotrys atra to apparent homogeneity. The induced enzyme was clearly different from the constitutive beta-D-glucosidase. The molecular weight was 65 500-69 000, the pH optimum was at 6.7 and the isoelectric point at 4.8. Carbohydrate content (related to glucose) was 14.4%. The enzyme showed beta-D-glucosidase, beta-D-xylosidase and beta-D-thioglucosidase activity. These three activities sppear to be due to the same enzyme. The enzyme was strongly inhibited by D-glucono-(1 leads to 5)-lactone and nojirimycin and was very sensitive to sulfhydryl reagents.
D017485:1-Deoxynojirimycin / Q000031:analogs & derivatives; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004228:Dithionitrobenzoic Acid / Q000494:pharmacology; D004790:Enzyme Induction; D005942:Gluconates / Q000494:pharmacology; D005944:Glucosamine / Q000494:pharmacology; D005959:Glucosidases / Q000378:metabolism*; D006601:Hexoses / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D003904:Mitosporic Fungi / Q000201:enzymology*; D008970:Molecular Weight; D013191:Stachybotrys / Q000201:enzymology*; D013237:Stereoisomerism; D013379:Substrate Specificity; D013696:Temperature; D001617:beta-Glucosidase / Q000302:isolation & purification / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D005942:Gluconates; D006601:Hexoses; D017485:1-Deoxynojirimycin; D004228:Dithionitrobenzoic Acid; D005959:Glucosidases; D001617:beta-Glucosidase; D005944:Glucosamine; C013626:nojirimycin