Regulation of enzymes of ethanol metabolism in yeast (Rhodotorula gracilis).
The three enzymes of ethanol metabolism alcohol dehydrogenase, aldehyde dehydrogenase and acetyl-CoA synthetase in the obligate aerobic yeast Rhodotorula gracilis are repressed by glucose and induced by C2 metabolic fuels with a regulatory pattern indicating a correlation in the control mechanisms. To try an identification of the molecular signals involved in the transmission of the inducing stimulus, experiments were carried out by blocking with 2 mM pyrazole the ethanol acetaldehyde metabolic step. Results indicate that ethanol is not specifically required as a molecular signal for induction.
D000079:Acetaldehyde / Q000494:pharmacology; D000106:Acetate-CoA Ligase / Q000096:biosynthesis*; D000085:Acetates / Q000494:pharmacology; D000429:Alcohol Oxidoreductases / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D000445:Aldehyde Oxidoreductases / Q000096:biosynthesis*; D003066:Coenzyme A Ligases / Q000096:biosynthesis*; D004790:Enzyme Induction / Q000187:drug effects; D000431:Ethanol / Q000378:metabolism* / Q000494:pharmacology; D005809:Genes, Regulator; D011720:Pyrazoles / Q000494:pharmacology; D015003:Yeasts / Q000201:enzymology / Q000378:metabolism*
D000085:Acetates; D011720:Pyrazoles; D000431:Ethanol; D000429:Alcohol Oxidoreductases; D000445:Aldehyde Oxidoreductases; D003066:Coenzyme A Ligases; D000106:Acetate-CoA Ligase; D000079:Acetaldehyde