Effects of phospholipids in the action of acetyl-CoA carboxylase from rat liver.
Acetyl-CoA carboxylase (E.C. 6.4.1.2) was isolated from rat liver. The purified enzyme contains phospholipids with a rather large amount of phosphatidylinositol (26%). Incubation of the purified acetyl-CoA carboxylase with phospholipase A2 (E.C. 3.1.1.4) or with phospholipase D (E.C. 3.1.1.4) diminishes the phospholipid content by 70%, this treatment leading to a complete inactivation of the enzyme. After removal of the phospholipases, the lipid-depleted enzyme can be reactivated to a certain degree by incubation with a phospholipid extract from rat liver, with phosphatidylinositol alone, or with serum albumin.
D000103:Acetyl-CoA Carboxylase / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D004789:Enzyme Activation; D008025:Ligases / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D010716:Phosphatidylinositols / Q000378:metabolism / Q000494:pharmacology*; D010740:Phospholipases; D010743:Phospholipids / Q000378:metabolism; D011485:Protein Binding; D051381:Rats; D012709:Serum Albumin / Q000378:metabolism
D010716:Phosphatidylinositols; D010743:Phospholipids; D012709:Serum Albumin; D010740:Phospholipases; D008025:Ligases; D000103:Acetyl-CoA Carboxylase