Organic solvents and temperature effects on desorption from immunoadsorbents. DNP-BSA anti-DNP as a model.
Conditions for desorbing a DNP carrier from its immunoadsorbent were studied in various hydro-organic media at different pHs and temperatures, including the sub-zero range. It appeared that DNP--anti-DNP interaction is the result of a balance between various bonds and the best result (95% yield) was obtained in hydro-organic solvent at high pH and +30 degrees C.
D000936:Antigen-Antibody Complex; D004136:Dinitrobenzenes; D006863:Hydrogen-Ion Concentration; D007164:Immunosorbents; D008954:Models, Biological; D012710:Serum Albumin, Bovine; D012997:Solvents; D013696:Temperature
D000936:Antigen-Antibody Complex; D004136:Dinitrobenzenes; D007164:Immunosorbents; D012997:Solvents; D012710:Serum Albumin, Bovine