Amino acid metabolizing enzymes in rat submaxillary gland, normal or neoplastic, and in pancreas.
The activities of 12 enzymes, many related to ornithine metabolism, were measured in rat submaxillary gland, submaxillary gland tumors and pancreas. In submaxillary gland, the activities of arginase, ornithine aminotransferase, pyrroline-5-carboxylate reductase and glutamine synthetase were high, but no ornithine transcarbamylase or proline oxidase could be detected. In the fetal submaxillary gland, arginase was at almost adult levels while ornithine aminotransferase reached 50% of its adult value postnatally. Submaxillary tumors deviated from their cognate tissue by lower levels of amino acid metabolizing enzymes and by high concentrations of thymidine kinase. In pancreas, none of the pyrroline-5-carboxylate metabolizing enzymes were as high as in either liver or submaxillary gland. The outstanding activities were those of gamma-glutamyl transpeptidase and glutamate dehydrogenase. Although arginase activities in submaxillary gland and pancreas were quantitatively similar, they differed qualitatively: submaxillary gland contained the same variant as liver while the pancreatic isozymes resembled those of other nonhepatic tissues.
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D001119:Arginase / Q000378:metabolism; D002277:Carcinoma / Q000201:enzymology; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D008297:Male; D009374:Neoplasms, Experimental / Q000201:enzymology; D009928:Organ Specificity; D009954:Ornithine Carbamoyltransferase / Q000378:metabolism; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000378:metabolism; D010179:Pancreas / Q000201:enzymology*; D051381:Rats; D012468:Salivary Gland Neoplasms / Q000201:enzymology*; D012513:Sarcoma, Experimental / Q000201:enzymology; D013363:Submandibular Gland / Q000201:enzymology; D013937:Thymidine Kinase / Q000378:metabolism; D000637:Transaminases / Q000378:metabolism; D005723:gamma-Glutamyltransferase / Q000378:metabolism
D000596:Amino Acids; D005969:Glutamate Dehydrogenase; D000587:Oxidoreductases Acting on CH-NH Group Donors; D009954:Ornithine Carbamoyltransferase; D005723:gamma-Glutamyltransferase; D000637:Transaminases; D013937:Thymidine Kinase; D001119:Arginase; D005974:Glutamate-Ammonia Ligase