Use of an enzymatic kit method for cholesterol, designed for continuous-flow instrumentation, with discrete bichromatic and centrifugal analyzers.
I examined a cholesterol-assay kit ("Autoflo", Biodynamics/bmc) designed for use with continuous-flow instrumentation for its use with some discrete analyzers. At pH 7.2, color develops within 3 min upon exposure to cholesterol, then begins to fade. At pH 8, the color develops more slowly but remains stable for at least 15 min after reaching a maximum. A pH 8.8, the color develops still more slowly and standards and patients' sera behave differently. Reagent at pH 7.2 is preferred because assays can be completed most rapidly, but pH 8 reagent must be used with instruments requiring longer periods of incubation. Cholesterol assays can be performed accurately, rapidly, reproducibly, and at low cost with an Abbott ABA-50 and pH 7.2 reagent or with a Gemeni Centrifugal Analyzer and the pH 8 reagent.
D001322:Autoanalysis / Q000379:methods; D002498:Centrifugation / Q000379:methods; D002784:Cholesterol / Q000097:blood*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D011933:Reagent Kits, Diagnostic; D013053:Spectrophotometry
D011933:Reagent Kits, Diagnostic; D002784:Cholesterol