NADPH dependent lipid peroxidation of calcium bound microsomes.
Rat liver microsomes prepared by a calcium aggregation method have been found capable of readily undergoing NADPH induced lipid peroxidation. Malonic dialdehyde production of calcium bound microsomes is equivalent to that attained by microsomes prepared by ultracentrifugation. In addition these calcium bound microsomes can be quickly removed from suspension by means of ultrafiltration.
D000818:Animals; D002118:Calcium / Q000502:physiology*; D005374:Filtration; D066298:In Vitro Techniques; D050356:Lipid Metabolism; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D009249:NADP / Q000378:metabolism*; D010545:Peroxides / Q000378:metabolism; D051381:Rats
D010545:Peroxides; D009249:NADP; D002118:Calcium