Ribonucleic acid polymerase activity associated with purified calf rotavirus.
The presence of an RNA-dependent RNA polymerase is demonstrated in purified rotavirus particles. Optimum polymerase activity was found between 45 to 50 degrees C, at pH 8, and in the presence of 10 mM-magnesium ions. The polymerase product was highly sensitive to pancreatic RNase (97%) in low or high salt concentration. The enzyme was activated by EDTA treatment of intact particles or heat shock. The similarities between reovirus, blue-tongue virus and rotavirus polymerases are discussed.
D000818:Animals; D001820:Bluetongue virus / Q000201:enzymology; D002417:Cattle; D006863:Hydrogen-Ion Concentration; D012316:RNA Nucleotidyltransferases / Q000032:analysis*; D012328:RNA Viruses / Q000201:enzymology*; D012367:RNA, Viral / Q000096:biosynthesis; D012324:RNA-Dependent RNA Polymerase / Q000032:analysis*; D012087:Reoviridae / Q000201:enzymology; D012401:Rotavirus / Q000201:enzymology* / Q000235:genetics; D013696:Temperature
D012367:RNA, Viral; D012316:RNA Nucleotidyltransferases; D012324:RNA-Dependent RNA Polymerase