Regulation of the oxidative NADP-enzyme tissue levels in Drosophila melanogaster. I. Modulation by dietary carbohydrate and lipid.
Wild-type third instar larvae of Drosophilia melanogaster fed a casein-sucrose synthetic diet supplemented with phosphatidylcholine (4 mg/ml) possessed 33% more tissue lipid and a modified fatty acid profile compared to larvae fed a fat free-sucrose diet. The rates of lipid synthesis and pentose shunt activity were 2.1 and 2.2 times greater respectively in larvae fed the fat free-sucrose diet than in fat-sucrose fed animals. The tissue concentrations of acetyl-CoA and acyl-CoA were 80 and 61% higher respectively, CoA 49% lower, and the NADPH/NADP+ ratio greater in fat-sucrose fed larvae than in larvae fed a fat free-sucrose diet. Thus, larvae effectively utilized dietary lipid for lipid synthesis and as a supplementary energy source to carbohydrate.
D000105:Acetyl Coenzyme A / Q000378:metabolism; D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D000818:Animals; D002364:Caseins / Q000378:metabolism; D003065:Coenzyme A / Q000378:metabolism; D004331:Drosophila melanogaster / Q000201:enzymology*; D005227:Fatty Acids / Q000096:biosynthesis; D005947:Glucose / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D007521:Isocitrate Dehydrogenase / Q000378:metabolism; D050356:Lipid Metabolism; D009249:NADP / Q000378:metabolism; D010713:Phosphatidylcholines / Q000378:metabolism; D010734:Phosphogluconate Dehydrogenase / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism
D002364:Caseins; D005227:Fatty Acids; D010713:Phosphatidylcholines; D009249:NADP; D013395:Sucrose; D000105:Acetyl Coenzyme A; D000429:Alcohol Oxidoreductases; D007521:Isocitrate Dehydrogenase; D010734:Phosphogluconate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D005947:Glucose; D003065:Coenzyme A