[Identification and properties of 2 forms of plant phospholipase D].
Effect of different factors (pH, temperature etc.) on the activity of phospholipase D is studied. It is found that plant phospholipase D has two forms: thermolabile (D1) and thermostable (Ds). Both forms are shown to differ in some characteristics (pH optimum, temperature optimum, calcium ions activation, activator effect, kinetic parameters, thermostavility). The contradictive literature data on phospholipase D properties are suggested to be due to the existence of two enzyme forms.
D002118:Calcium / Q000494:pharmacology; D004355:Drug Stability; D004789:Enzyme Activation; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010740:Phospholipases / Q000378:metabolism*; D010944:Plants / Q000201:enzymology*; D013696:Temperature
D010740:Phospholipases; D002118:Calcium
Obnaruzhenie i svoĭstva dvukh form fosfolipazy D rastenii.