New substrate for fluorometric determination of gamma-glutamyltransferase activity in serum.
We describe the preparation of a new substrate, N-gamma-L-glutamyl-5-aminoisophthalic acid dimethyl ester hydro-chloride, for the fluorometric determination of gamma-glutamyltransferase activity in serum by the "front-surface" technique. Details of the resulting method are provided. The final reaction mixture contains 4 mmol of the substrate per liter of tris(hydroxymethyl)aminomethane (100 mmol/liter) and glycylglycine (75 mmol/liter) at pH 8.2 (25 degrees C).
D002021:Buffers; D003124:Colorimetry; D005470:Fluorometry / Q000295:instrumentation; D006863:Hydrogen-Ion Concentration; D008722:Methods; D010795:Phthalic Acids / Q000138:chemical synthesis; D011803:Quinine; D013050:Spectrometry, Fluorescence; D005723:gamma-Glutamyltransferase / Q000097:blood*
D002021:Buffers; D010795:Phthalic Acids; D011803:Quinine; D005723:gamma-Glutamyltransferase