Chemotactic activity of guinea pig eosinophils for the ECF-A acidic tetrapeptides, histamine, histamine metabolites, and the effect of H1- and H2-receptor antagonists.
Histamine and one of its major metabolites, imidazoleacetic acid, were selectively chemotactic for guinea pig eosinophils, whereas L-histidine, 1,4-methylimidazoleacetic acid, 1,4-methylhistamine and N-acetylhistamine were inactive. The response to histamine was unaffected by concentrations of eosinophils of between 30 and greater than 90% but it was abrogated by preincubation of the cells with histamine prior to assay (self-deactivation). Eosinophilotaxis was also inhibited by H1-(mepyramine-) and H2-)burimamide)-receptor antagonists at high doses (10(-3)m), although at lower concentrations (10(-5) M) inhibition was principally associated with burimamide. The human tetrapeptide, alanine-glycine-serine-glutamic acid, and the analogue, valine-glycine-aspartic acid-glutamic acid, were inactive whereas alanine-glycine-serine-glutamic acid was chemotactic for the guinea pig eosinophil. These results support the concept that the tissue accumulation of eosinophils following anaphylaxis depends on a complex interaction of factors, which in part may be mediated by H2 receptors on the target cells. There may be species differences in the composition of ECF-A.
D000409:Alanine; D000707:Anaphylaxis / Q000276:immunology*; D000818:Animals; D002049:Burimamide / Q000494:pharmacology; D002634:Chemotaxis, Leukocyte; D004306:Dose-Response Relationship, Immunologic; D004804:Eosinophils; D005260:Female; D005971:Glutamates / Q000494:pharmacology; D006168:Guinea Pigs; D006632:Histamine / Q000494:pharmacology*; D006634:Histamine H1 Antagonists / Q000494:pharmacology*; D006635:Histamine H2 Antagonists / Q000494:pharmacology*; D006639:Histidine / Q000494:pharmacology; D007093:Imidazoles / Q000494:pharmacology; D008297:Male; D008761:Methylhistamines / Q000494:pharmacology; D009842:Oligopeptides / Q000494:pharmacology*; D011738:Pyrilamine / Q000494:pharmacology; D014633:Valine
D005971:Glutamates; D006634:Histamine H1 Antagonists; D006635:Histamine H2 Antagonists; D007093:Imidazoles; D008761:Methylhistamines; D009842:Oligopeptides; D006639:Histidine; D006632:Histamine; D014633:Valine; D011738:Pyrilamine; D000409:Alanine; D002049:Burimamide