Hydrolytic enzymes of Euglena gracilis: characterization and activity as a function of culture age and carbon deprivation.
Optimal assay conditions are described for 8 hydrolases of Euglena gracilis var. bacillaris, SM-L1 (streptomycin-bleached) strain, 7 of which have an acid pH-optimum. Acid-phosphatase, beta-galactosidase, beta-glucosidase, b-fucosidase, cathepsin D, RNase, DNase, and an esterase are active in cell homogenates. Amylase has very low activity, and beta-glucuronidase, arylsulfatase, beta, N-acetyl-glucosaminidase, alpha-fucosidase, and alpha- and beta-mannosidase are inactive.
D000085:Acetates / Q000378:metabolism; D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D002244:Carbon / Q000378:metabolism*; D002403:Cathepsins / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism; D004950:Esterases / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism; D005056:Euglena gracilis / Q000201:enzymology* / Q000254:growth & development; D005696:Galactosidases / Q000378:metabolism; D005959:Glucosidases / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006867:Hydrolases / Q000378:metabolism*
D000085:Acetates; D000431:Ethanol; D002244:Carbon; D006867:Hydrolases; D003851:Deoxyribonucleases; D004950:Esterases; D000135:Acid Phosphatase; D005696:Galactosidases; D005959:Glucosidases; D002403:Cathepsins