The interaction of N-acetylhexosaminidase with insolubilized concanavalin A.
The specific interaction between human N-acetylhexosaminidase and concanavalin A was evaluated with respect to temperature, time, pH and concentration of specific ligand in incubation mixtures containing the enzyme and insolubilized lectin. Elution of the enzyme from insolubilized concanavalin A is dependent on both temperature and concentration of alpha-methyl mannoside. Conditions for a high yield of the enzyme from chromatography on insolubilized concanavalin A are described.
D055598:Chemical Phenomena; D002621:Chemistry; D003208:Concanavalin A; D006596:Hexosaminidases; D006801:Humans; D006863:Hydrogen-Ion Concentration; D011485:Protein Binding; D013696:Temperature; D013995:Time
D003208:Concanavalin A; D006596:Hexosaminidases