L-Glutamate-glyoxylate aminotransferase in Lactobacillus plantarum.
Glutamate-glyoxylate aminotransferase which mediates the reaction of glyoxylic acid with glutamic acid to yield glycine and alpha-oxoglutaric acid has been isolated and purified 84-fold from extracts of Lactobacillus plantarum. Purified enzyme requires the addition of pyridoxal phosphate and magnesium ions for its activity. The molecular weight of the enzyme estimated by Sepharose 4B gel filtration amounts to 37.000. Micaelis constants for glyoxylate and glutamate are corresponding to 6.25 X 10(-3) M and 2.75 X 10(-3) M, respectively. Optimal pH in phosphate and veronal buffers is 8.0 and optimal temperature 35--37 degrees C.
D001386:Azides / Q000494:pharmacology; D002474:Cell-Free System; D003486:Cyanides / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D005971:Glutamates / Q000378:metabolism; D005998:Glycine / Q000096:biosynthesis; D006038:Glyoxylates / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006898:Hydroxylamines / Q000494:pharmacology; D007538:Isoniazid / Q000494:pharmacology; D007656:Ketoglutaric Acids / Q000096:biosynthesis; D007778:Lactobacillus / Q000201:enzymology*; D008274:Magnesium / Q000378:metabolism; D011732:Pyridoxal Phosphate / Q000378:metabolism; D013696:Temperature; D000637:Transaminases / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism
D001386:Azides; D003486:Cyanides; D005971:Glutamates; D006038:Glyoxylates; D006898:Hydroxylamines; D007656:Ketoglutaric Acids; D011732:Pyridoxal Phosphate; D004492:Edetic Acid; D000637:Transaminases; D008274:Magnesium; D005998:Glycine; D007538:Isoniazid