[Determination of barbiturates from biological material].
A trace quantitative analysis of barbiturates has been carried out in blood, urine, organs and in gastric and intestinal concents. The amount of sample required for analysis is very small [approximately 400 mul blood]. Extraction is carried out four times with the mixture of acetone and either 1:1]. The preparation of columns, packing and standards has been described. The device used for the analyses [Chrom 3] is furnished with an adjusted feeding block preventing the decomposition of samples in the doser. The column temperature is 190 degrees C, the column packing is Chromaton N-AW-DMCS coated with 3% NPGS and 0.75% trimer acid, detector FID.
D000654:Amobarbital / Q000378:metabolism; D001462:Barbital / Q000378:metabolism; D001463:Barbiturates / Q000097:blood / Q000378:metabolism* / Q000652:urine; D002849:Chromatography, Gas; D005554:Forensic Medicine; D006591:Hexobarbital / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010424:Pentobarbital / Q000378:metabolism; D010634:Phenobarbital / Q000378:metabolism
D001463:Barbiturates; D001462:Barbital; D006591:Hexobarbital; D000654:Amobarbital; D010424:Pentobarbital; D010634:Phenobarbital
Stanovení barbiturátů z biologického materiálu