Microsomal glucuronidation of a quaternary ammonium compound, 3-hydroxyphenyltrimethylammonium.
3-Hydroxyphenyltrimethylammonium (HPTMA) is glucuronidated in vitro by rat liver microsomes supplemented with UDP-glucuronic acid. Little reaction occurred in the postmicrosomal supernatant fraction. Triton X-100 doubled the maximal velocity for the microsomecatalyzed reaction without altering the apparent KM value for HPTMA. This is the first report of glucuronidation of a quaternary ammonium compound in vitro.
D000818:Animals; D002854:Chromatography, Paper; D005965:Glucuronates / Q000096:biosynthesis; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D010636:Phenols / Q000378:metabolism; D011092:Polyethylene Glycols / Q000494:pharmacology; D000644:Quaternary Ammonium Compounds / Q000378:metabolism*; D051381:Rats
D005965:Glucuronates; D010636:Phenols; D000644:Quaternary Ammonium Compounds; D011092:Polyethylene Glycols