Distribution and biliary excretion products of di-2-ethylhexyl phthalate in rainbow trout.
After 24-hr exposures of rainbow trout to 0.5 ppm of di-2-ethylhexyl [14C]phthalate, one-half of the radioactivity present in the fish was localized in the bile. The bile was pooled and fractionated by selective solvent extraction before and after beta-glucuronidase hydrolysis. Individual radioactive compounds were further separated and characterized by thin-layer chromatography. Gas chromatography-mass spectrometry was used to confirm the results of thin-layer chromatographic analysis. These procedures demonstrated that bile contained a number of DEHP metabolites, but only about 1% of unchanged DEHP. The major metabolite, mono-2-ethylhexyl phthalate glucuronide accounted for 72% of the total bile radioactivity. The remaining bile radioactivity was found to be present as phthalic acid glucuronide, mono-2-ethylhexyl phthalate and two partially characterized polar metabolites.
D000818:Animals; D001646:Bile / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D004051:Diethylhexyl Phthalate / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D005965:Glucuronates / Q000378:metabolism; D005966:Glucuronidase; D009929:Organ Size; D009928:Organ Specificity; D010795:Phthalic Acids / Q000378:metabolism*; D012487:Salmonidae / Q000378:metabolism*; D014337:Trout / Q000378:metabolism*
D005965:Glucuronates; D010795:Phthalic Acids; D004051:Diethylhexyl Phthalate; D005966:Glucuronidase