6-Phospho-D-gluconate:NAD+ 2-oxidoreductase (decarboxylating) from slow-growing Rhizobia.
6-Phospho-D-gluconate:NAD+ 2-oxidoreductase (decarboxylating) (NAD+-6PGD) was detected in several slow-growing strains of rhizobia, and no activity involving NADP+ was found in the same extracts. By contrast, fast-growing strains of rhizobia had NADP+-6PGD activity; most of them also had NAD+-6PGD activity. NAD+-6PGD was partially purified from the slow-growing strain Rhizobium japonicum 5006. The reaction was shown to be an oxidative decarboxylation.
D002474:Cell-Free System; D003653:Decarboxylation; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009243:NAD / Q000494:pharmacology; D010084:Oxidation-Reduction; D010734:Phosphogluconate Dehydrogenase / Q000302:isolation & purification* / Q000378:metabolism; D012231:Rhizobium / Q000201:enzymology* / Q000254:growth & development; D013997:Time Factors
D009243:NAD; D010734:Phosphogluconate Dehydrogenase