[Role of divalent cations in the structural organization of the peroxisomal membrane].
Factors, which affected the stability of peroxisomal membrane in vitro, were investigated. The decrease in the activity of catalase at neutral and alkaline pH was prevented by addition of Ca2+, Mg2+ and Ba2+; at the same time EDTA, EGTA and o-phenantroline decreased the stability of peroxisomes. All the effects were temperature-dependent. Ca2+ did not prevent the injury of peroxisomes during hypotonic lysis, but increased the electrostatic interactions of enzymes with the peroxisomal membrane. These data suggest that at neutral and alkaline pH the peroxisomal membranes had a large negative charge on their surface, which affected the stability of peroxisomes in vitro.
D000135:Acid Phosphatase / Q000378:metabolism; D000445:Aldehyde Oxidoreductases / Q000378:metabolism; D000818:Animals; D002374:Catalase / Q000378:metabolism; D002413:Cations, Divalent; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure*; D002614:Chelating Agents / Q000494:pharmacology; D003576:Electron Transport Complex IV / Q000378:metabolism; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D006016:Glycolates / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000648:ultrastructure; D008297:Male; D008830:Microbodies / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure*; D009940:Organoids / Q000648:ultrastructure*; D051381:Rats; D014503:Urate Oxidase / Q000378:metabolism
D002413:Cations, Divalent; D002614:Chelating Agents; D006016:Glycolates; D007770:L-Lactate Dehydrogenase; D002374:Catalase; D000445:Aldehyde Oxidoreductases; D014503:Urate Oxidase; D003576:Electron Transport Complex IV; D000135:Acid Phosphatase; D005952:Glucose-6-Phosphatase
Rol' dvukhvalentnykh kationov v strukturnoĭ organizatsii peroksisomal'noĭ membrany