Measurement of antibodies to herpesvirus types 1 and 2 in human sera by microradioimmunoassay.
The binding of 125I-labelled anti-human antibodies against the fc IgG fragment to unlabelled antiviral immunoglobulins in the surface of infected cells was used to quantitate antibodies against herpes simplex virus type (HSV-1) and type 2 (HSV-2) in sera from patients with cervix carcinoma. The microradioimmunoassay technique (micro-RIA) proved to be 5-10 times more sensitive than the microneutralization test. Antibody titres determined by micro-RIA correlated with neutralizing antibody titres to both HSV-1 and HSV-2. The relative antibody titres to HSV-1 and HSV-2, as determined by micro-RIA, could be used to distinguish persons previously infected with HSV-2 by means of II/I indices.
D000914:Antibodies, Viral / Q000032:analysis*; D003937:Diagnosis, Differential; D005260:Female; D006561:Herpes Simplex / Q000175:diagnosis; D006801:Humans; D009500:Neutralization Tests; D011863:Radioimmunoassay; D018139:Simplexvirus / Q000276:immunology*; D002583:Uterine Cervical Neoplasms / Q000276:immunology*
D000914:Antibodies, Viral