A stabilising factor for gamma-glutamyl transpeptidase in urine.
Urinary gamma-glutamyl transpeptidase (gamma-GT) has been found to be stable when stored at room temperature and 4 degrees C. Activity is lost rapidly when urine is frozen but prior dialysis will prevent this loss. Urea is the major factor responsible for the loss of activity; albumin is protective at concentrations of 6 g/l or more. A factor of 10 000-30 000 molecular weight which will prevent the loss of urinary gamma-GT activity on freezing has been found in serum and urine; it has high potency in serum and in urine from patients with chronic renal failure, but only low potency in normal urine. Its nature is unknown but it is heat stable.
D000418:Albumins / Q000494:pharmacology; D001769:Blood; D003956:Dialysis; D004355:Drug Stability; D004492:Edetic Acid / Q000494:pharmacology; D005615:Freezing; D007676:Kidney Failure, Chronic / Q000201:enzymology; D008970:Molecular Weight; D013696:Temperature; D014462:Ultrafiltration; D005723:gamma-Glutamyltransferase / Q000652:urine*
D000418:Albumins; D004492:Edetic Acid; D005723:gamma-Glutamyltransferase