Influence of changes in arterial PCO2 on cerebral blood flow and cerebral energy state during hypothermia in the rat.
In order to study the relationship between arterial PCO2 and cerebral blood flow (CBF) in hypothermia, the body temperature of artifically ventilated rats was decreased to 22 degreesC, and changes in CBF were evaluated from arteriovenous differences in oxygen content (AVDO2) at PaCO2 values of 15, 30, 40 and 60 mm Hg. The results were compared to those obtained at normal body temperature (37 degrees C) over the PaCO2 range 15-60 mm Hg. Separate experiments were performed to evaluate CBF and CMRO2 at 22 degrees C and a PaCO2 of 15 mm Hg, using an inert gas technique for CBF. The tissue contents of phosphocreatine, ATP, ADP, AMP and lactate were measured in hypothermic animals at PaCO2 values of 15, 30 and 60 mm Hg. The results showed that changes in CBF were of the same relative magnitude in hypothermia and normothermia when PaCO2 was increased from about 35 to about 60 mm Hg. However, with a decrease in PaCO2 the reduction in CBF was much more pronounced in hypothermia, and at PaCO2 15 Mm Hg CBF was less then 20% of the value measured in normothermic and normocapnic animals. The results of the metabolite measurements gave no evidence of tissue hypoxia in spite of the pronounced reduction in CBF. Although the results demonstrate that the brain of a hypothermic animal is protected against the harmful effects of a lowered CBF, it may not warrant recommending hyperventilation in clinical cases of hypothermia, especially not in patients with arteriosclerosis or cerebrovascular diseases.
D000244:Adenosine Diphosphate / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001158:Arteries; D001794:Blood Pressure; D001921:Brain / Q000378:metabolism*; D002245:Carbon Dioxide / Q000097:blood*; D002540:Cerebral Cortex / Q000378:metabolism; D002560:Cerebrovascular Circulation; D004734:Energy Metabolism; D006863:Hydrogen-Ion Concentration; D007036:Hypothermia, Induced; D007773:Lactates / Q000378:metabolism; D008297:Male; D010100:Oxygen / Q000097:blood; D010313:Partial Pressure; D010725:Phosphocreatine / Q000378:metabolism; D051381:Rats; D012039:Regional Blood Flow / Q000187:drug effects
D007773:Lactates; D010725:Phosphocreatine; D002245:Carbon Dioxide; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D010100:Oxygen