Measurement of the alpha-mannosidase activities in human plasma by a differential assay.
A differential assay based on their difference in thermal stability has been used to measure the acidic and true intermediate alpha-mannosidases in the plasma of cntrols and individuals homozygous or heterozygous for mannosidosis. The intermediate activity was found to be independent of age, sex or mannosidosis genotype. The acidic alpha-mannosidase did not vary significantly with age or between sexes for groups of the same age. The concentrations of acidic and intermediate alpha-mannosidase showed a positive correlation for adults but not for children. The ratio of acidic to true intermediate alpha-mannosidase might therefore be a useful secondary test for the detection of adult heterozygotes for mannosidosis.
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D002239:Carbohydrate Metabolism, Inborn Errors / Q000097:blood / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D003035:Cobalt / Q000494:pharmacology; D005260:Female; D005838:Genotype; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007223:Infant; D007700:Kinetics; D008297:Male; D008358:Mannose / Q000378:metabolism; D008361:Mannosidases / Q000097:blood*; D008722:Methods; D012737:Sex Factors; D015032:Zinc / Q000494:pharmacology
D003035:Cobalt; D008361:Mannosidases; D015032:Zinc; D008358:Mannose