Transfection of Streptococcus pneumoniae with bacteriophage DNA.
It was possible to transfect Streptococcus pneumoniae with DNA obtained from a newly isolated bacteriophage, diplophage-4 (Dp-4). Optimal frequency of transfection (0.9%) required the use of a nuclease-defective mutant; with wild-type bacteria, the transfection frequency was about 100-fold lower. Transfection requires physiological conditions that appear to be similar to the competent state needed for genetic transformation (A. Tomasz, J. Bacteriol. 91:1050--1061, 1966).
D001435:Bacteriophages / Q000235:genetics* / Q000254:growth & development; D002701:Chloramphenicol / Q000494:pharmacology; D004279:DNA, Viral; D006863:Hydrogen-Ion Concentration; D013296:Streptococcus pneumoniae / Q000235:genetics*; D014162:Transfection; D014357:Trypsin / Q000494:pharmacology; D010948:Viral Plaque Assay
D004279:DNA, Viral; D002701:Chloramphenicol; D014357:Trypsin