Immunological properties of the lipopolysaccharide-protein complex of Coxiella burnetii.
Purified lipopolysaccharide-protein complex (LPS-PC) extracted by trichloroacetic acid from phase I Coxiella burnetii organisms induced in mice and rabbits fair levels of antibodies directed to antigen 1 and antigen 2, as detected by complement-fixation (CF), microagglutination (MA), opsonization-phagocytosis (OP) and serum protection (SP) tests. In guinea pigs only very low levels of MA antibodies against antigen 2 were demonstrated. In rabbit serum, MA antibodies directed to antigen 2 were found exclusively in the IgM fraction after the primary immunizing dose; the second dose was followed by gradual shift of MA antibodies to the IgG class. Two immunizing doses of the LPS-PC were more effective when testing antibody response in mice or protection of mice and guinea pigs against phase I virulent challenge.
D000372:Agglutination Tests; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D001426:Bacterial Proteins / Q000276:immunology*; D003168:Complement Fixation Tests; D003381:Coxiella / Q000276:immunology*; D006168:Guinea Pigs; D007114:Immunization; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000276:immunology*; D051379:Mice; D010587:Phagocytosis; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D011778:Q Fever / Q000517:prevention & control; D011817:Rabbits
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial