The use of tris-HCl buffer for inoculation of tomato protoplasts with tobacco mosaic virus.
When tris-HCL was used as the buffer for inoculation of tomato protoplasts with tobacco mosaic virus, a greater proportion became infected than when phosphate was used. Using 0-05 M-tris-HCl buffer, pH 8-0, in the presence of poly-L-ornithine or poly-D-lysine, 50 to 80 % infection was obtained.
D002021:Buffers; D006851:Hydrochloric Acid / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D009952:Ornithine; D010455:Peptides / Q000494:pharmacology; D010710:Phosphates / Q000494:pharmacology; D010935:Plant Diseases; D010944:Plants / Q000382:microbiology*; D011107:Polylysine / Q000494:pharmacology; D011523:Protoplasts / Q000382:microbiology*; D013237:Stereoisomerism; D014027:Tobacco Mosaic Virus / Q000254:growth & development*; D014325:Tromethamine / Q000494:pharmacology; D014675:Vegetables
D002021:Buffers; D010455:Peptides; D010710:Phosphates; D014325:Tromethamine; D011107:Polylysine; D009952:Ornithine; D006851:Hydrochloric Acid