Effects of fatty acids on superoxide radical generation in leukocytes.
Acetylated ferricytochrome c was employed for the detection of superoxide radicals (O-2) generated both in intact cells and in subcellular fractions of leukocytes. Certain saturated fatty acids, myristate in particular, induced the production of O-2 in leukocytes, suggesting a correlation between the formation of O-2 and the hydrophobic interaction of fatty acids with the leukocyte plasma membrane. As compared with O-2 radical generation from phagocytizing leukocytes a greater stimulation of O-2 formation was observed in cells in which myristate was added. The enhanced activity which generated O-2 in the cell-free system was located in a particulate fraction but not in the cytosol. The rate of O-2 generation in the particulate fraction was higher in the presence of NADPH than in the presence of NADH. The effects of reagents such as KCN, 2,4-dichlorophenol and aminotriazole on the O-2 generation in this fraction are examined and the nature of the O-2 generating system is discussed.
D000640:Amitrole / Q000494:pharmacology; D000818:Animals; D002374:Catalase / Q000494:pharmacology; D002733:Chlorophenols / Q000494:pharmacology; D006224:Cricetinae; D003486:Cyanides / Q000494:pharmacology; D003574:Cytochrome c Group / Q000378:metabolism; D005227:Fatty Acids / Q000494:pharmacology*; D007962:Leukocytes / Q000378:metabolism*; D009226:Myristates / Q000494:pharmacology; D009249:NADP / Q000494:pharmacology; D010100:Oxygen / Q000097:blood*; D013347:Subcellular Fractions / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000097:blood*
D002733:Chlorophenols; D003486:Cyanides; D003574:Cytochrome c Group; D005227:Fatty Acids; D009226:Myristates; D013481:Superoxides; D009249:NADP; D002374:Catalase; D013482:Superoxide Dismutase; D010100:Oxygen; D000640:Amitrole