Polynucleotides. XL. Synthesis and properties of poly 2'-azido-2'-deoxyadenylic acid.
Poly 2'-azido-2'-deoxyadenylic acid (Poly Az) was synthesized from 2'-azido-2'-deoxyadenosine diphosphate by polynucleotide phosphorylase. Poly (Az) has U.V. absorption properties similar to poly (A) and hypochromicity of 40% at 0.1 M Na+ and neutrality. CD curve also resembled to that of poly (A), but has smaller ellipticity. Titration of poly (Az) with HCl gave a transition at pH 5.5, but exact structure of the acid-form complex was not elucidated. Upon mixing with poly (U), poly (Az) forms a 1:1 and 1:2 complexes having Tm's somewhat higher than that of poly (A)- poly (U) complex in the same condition.
D001386:Azides; D002942:Circular Dichroism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009690:Nucleic Acid Conformation; D011061:Poly A / Q000031:analogs & derivatives / Q000138:chemical synthesis; D011089:Polydeoxyribonucleotides / Q000138:chemical synthesis; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature
D001386:Azides; D011089:Polydeoxyribonucleotides; D011061:Poly A