Rat liver cells in culture: effect of storage, long-term culture, and transformation on some enzyme levels.
Aryl hydrocarbon hydroxylase (AHH) and tyrosine aminotransferase (TAT) activities were determined in rat liver cell lines after frozen storage, long-term culture, and transformation in vitro. Levels of AHH activity after 17 months in frozen storage were comparable to levels prior to freezing. During long-term culture the AHH levels of the cell lines tended to decrease. Transformed lines had variable levels of AHH activity. Cell lines retained measurable TAT activity following long-term culture and frozen storage. TAT activity of transformed cells was comparable to that of normal lines. Prolonged frozen storage did not induce transformation up to one year.
D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000378:metabolism*; D001551:Benz(a)Anthracenes; D002455:Cell Division; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D003470:Culture Media; D004790:Enzyme Induction; D005615:Freezing; D008099:Liver / Q000166:cytology*; D008748:Methylcholanthrene; D009374:Neoplasms, Experimental / Q000209:etiology; D010634:Phenobarbital; D011309:Preservation, Biological; D051381:Rats; D014444:Tyrosine Transaminase / Q000378:metabolism*
D001551:Benz(a)Anthracenes; D003470:Culture Media; D008748:Methylcholanthrene; D001189:Aryl Hydrocarbon Hydroxylases; D014444:Tyrosine Transaminase; D010634:Phenobarbital