Adenosine triphosphate induced change in light scattering of isolated basal bodies.
Suspensions of isolated basal bodies undergo a characteristic decrease in turbidity following their exposure to 10(-3) M ATP. Typically, turbidity changes range from 8% to 20%, depending on the preparation, with an average change of 12%. Nucleotides other than ATP did not cause a turbidity decrease. The reaction has a pH optimum of pH 8.5 and is inhibited by concentrations of divalent cations greater than 2 X 10(-3) M. These results indicate that ATP induces a conformational change in the basal body that may be related to its activity in the cell.
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D002413:Cations, Divalent / Q000494:pharmacology; D002645:Chickens; D002923:Cilia / Q000648:ultrastructure; D005260:Female; D006863:Hydrogen-Ion Concentration; D009391:Nephelometry and Turbidimetry; D009711:Nucleotides / Q000494:pharmacology; D009940:Organoids / Q000187:drug effects* / Q000648:ultrastructure; D010057:Oviducts / Q000648:ultrastructure*; D011487:Protein Conformation / Q000187:drug effects; D013347:Subcellular Fractions
D002413:Cations, Divalent; D009711:Nucleotides; D000255:Adenosine Triphosphate