Enhancement of 5-iododeoxyuridine-induced endogenous C-type virus activation by polycyclic hydrocarbons: apparent lack of parallelism between enhancement and carcinogenicity.
When mouse MLg cells were treated with 3-methylcholanthrene or 7,12-dimethylbenz[alpha]anthracene in the presence of microsomal enzymes and NADPH after 5-iododeoxyuridine (IUDR) treatment, the induction rate of the endogenous C-type virus was increased fivefold to sixfold in comparison with the culture treated with IUDR only. In this reaction, both the microsomal enzymes and NADPH were indispensable. 7,8-Benzoflavone, an inhibitor of the metabolism of hydrocarbons in hamster embryo cultures, inhibited the reaction. For detecting the enhancing activity, the concentration of IUDR for the pretreatment, the concentration of the test products, and the duration of the treatment with the products were important factors. In screening 30 polycyclic hydrocarbons, we were unable to detect a correlation between the in vivo carcinogenicity in the skin and the enhancing activity in the conditions tested.
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000378:metabolism; D001580:Benzopyrenes / Q000494:pharmacology; D002273:Carcinogens / Q000494:pharmacology; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D004357:Drug Synergism; D005419:Flavonoids / Q000494:pharmacology; D007065:Idoxuridine / Q000008:administration & dosage / Q000494:pharmacology*; D066298:In Vitro Techniques; D007700:Kinetics; D008748:Methylcholanthrene / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D011083:Polycyclic Compounds / Q000494:pharmacology*; D012190:Retroviridae / Q000187:drug effects*; D014779:Virus Replication / Q000187:drug effects*
D001580:Benzopyrenes; D002273:Carcinogens; D005419:Flavonoids; D011083:Polycyclic Compounds; D009249:NADP; D008748:Methylcholanthrene; D015127:9,10-Dimethyl-1,2-benzanthracene; D006899:Mixed Function Oxygenases; D007065:Idoxuridine