The fluctuation of various enzyme activities due to myo-inositol deficiency in Saccharomyces carlsbergensis.
The neutral lipid accumulation in myo-inositol deficient Saccharomyces carlsbergensis results at least partly from an enhancement of acetyl CoA carboxylase activity due to the high level of fructose 1,6-biphosphate which activates acetyl CoA carboxylase, and due to the low level of citrate which counteracts the activation [4]. In an attempt to explore the effect of myo-inositol deficiency on the metabolic fluxes, various enzyme activities were compared between the myo-inositol supplemented and deficient cells. The activities of phosphofructokinase and ATP-citrate lyase increased by 74 and 83%, respectively. The activity of glucose-6-phosphate dehydrogenase was unchanged. Unlike acetyl CoA carboxylase, elimination of low molecular effectors had no influence on their activities. The thermostability of phosphofructokinase (at 53 degrees C) increased, while that of aldolase (at 48 degrees C) greatly decreased due to the deficiency. The thermostability of glucose-6-phosphate dehydrogenase (at 52 degrees C) was also unchanged.
D001275:ATP Citrate (pro-S)-Lyase / Q000378:metabolism; D000103:Acetyl-CoA Carboxylase / Q000378:metabolism; D002950:Citrate (si)-Synthase / Q000378:metabolism; D004355:Drug Stability; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D006019:Glycolysis; D007294:Inositol / Q000378:metabolism*; D010732:Phosphofructokinase-1 / Q000378:metabolism; D012440:Saccharomyces / Q000201:enzymology*; D013696:Temperature
D007294:Inositol; D005954:Glucosephosphate Dehydrogenase; D002950:Citrate (si)-Synthase; D001275:ATP Citrate (pro-S)-Lyase; D010732:Phosphofructokinase-1; D005634:Fructose-Bisphosphate Aldolase; D000103:Acetyl-CoA Carboxylase