[Spectrofluorometric determination of a new beta-blocking agent, acebutolol in blood and urine].
The authors describe a simple method of spectrofluorimetric estimation of acebutolol, applicable to blood and urine, sufficiently sensitive to obtain therapeutic concentrations. After administration of a 200 mg dose by mouth, one may observe a maximal plasma concentration 3 hours later, with average values of 1.02 +/- 0.20 mg/liter. The principle of the estimation is based on acid hydrolysis of the product, then condensation of the liberated amine with nitroso-1 naphtol-2 to give a fluorescent derivative (stimulation 460 nm, emission 545 nm).
D000070:Acebutolol / Q000032:analysis* / Q000097:blood / Q000652:urine; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008832:Microchemistry; D013050:Spectrometry, Fluorescence / Q000379:methods
D000070:Acebutolol
Dosage spectrofluorimétrique dans le sang et l'urine d'un nouveau bêta-bloquant, l'acébutolol