Reactive carriers of immobilized compounds.
Sphericanl macroporous reactive carriers capable of forming covalent bonds with amino acids and proteins were prepared by the suspension copolymerization of 2-hydroxyethyl methacrylate, ethylene dimethacrylate and p-nitrophenyl esters of methacrylic acid and methacryloyl derivatives of glycine, beta-alanine and epsilon-aminocaproic acid. The effect of the spacer length, pH and the type of the buffer used, concentration of reactive groups in the copolymer, concentration of the ligand and the participation of the hydrolytic and aminolytic reaction of p-nitrophenyl functional groups in the attachment of glycine, D,L-phenylalanine and serumalbumin was studied. Macroporous copolymers containing reactive functional groups can be used as active enzyme carriers, if their activity is not blocked by the presence of p-nitrophenol split off in the attachment reaction.
D002264:Carboxylic Acids; D004800:Enzymes, Immobilized / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008689:Methacrylates; D009596:Nitrophenols; D010206:Papain; D010649:Phenylalanine; D011485:Protein Binding; D013329:Structure-Activity Relationship
D002264:Carboxylic Acids; D004800:Enzymes, Immobilized; D008689:Methacrylates; D009596:Nitrophenols; D010649:Phenylalanine; D010206:Papain