beta-Hexosaminidase isozymes in human colonic carcinoma.
Isoelectric focusing of crude extracts demonstrated that human colonic carcinomas contained a higher proportion of beta-hexosaminidase B than beta-hexosaminidase A, while normal human colonic mucosa contained a higher proportion of the A form of the enzyme. Studies of the isolated isozymes showed that beta-hexosaminidase B was more stable to heat and more active at low pH than beta-hexosaminidase A. Kinetic studies revealed that the A and B forms of beta-hexosaminidase had essentially the same Km and Vmax for p-nitrophenyl-N-acetyl-beta-D-glucosaminide.
D000116:Acetylgalactosamine / Q000031:analogs & derivatives / Q000378:metabolism; D000117:Acetylglucosamine / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D003106:Colon / Q000201:enzymology; D003110:Colonic Neoplasms / Q000201:enzymology*; D006596:Hexosaminidases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007527:Isoenzymes / Q000037:antagonists & inhibitors / Q000378:metabolism*; D007700:Kinetics
D007527:Isoenzymes; D006596:Hexosaminidases; D000116:Acetylgalactosamine; D000117:Acetylglucosamine