Phospholipase D activity of gram-negative bacteria.
A phospholipase hydrolyzing cardiolipin to phosphatidic acid and phosphatidyl glycerol was characterized in gram-negative bacteria but was absent in preparations of gram-positive bacteria, Saccharomyces cerevisiae, and rat liver mitochondria. In cell-free extracts of Escherichia coli, Salmonella typhimurium, Proteus vulgaris, and Pseudomonase aeruginosa, this cardiolipin-hydrolyzing enzyme had similar pH and Mg2+ requirements and displayed a specificity which excluded phosphatidyl glycerol and phosphatidyl ethanolamine as substrates.
D000818:Animals; D001409:Bacillus cereus / Q000201:enzymology; D001412:Bacillus subtilis / Q000201:enzymology; D002308:Cardiolipins / Q000378:metabolism; D002474:Cell-Free System; D004926:Escherichia coli / Q000201:enzymology; D006088:Gram-Negative Aerobic Bacteria / Q000201:enzymology*; D006089:Gram-Negative Anaerobic Bacteria / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008274:Magnesium / Q000494:pharmacology; D008930:Mitochondria, Liver / Q000201:enzymology; D010712:Phosphatidic Acids / Q000096:biosynthesis; D010715:Phosphatidylglycerols / Q000096:biosynthesis; D010740:Phospholipases / Q000378:metabolism*; D011514:Proteus vulgaris / Q000201:enzymology; D011550:Pseudomonas aeruginosa / Q000201:enzymology; D051381:Rats; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D012486:Salmonella typhimurium / Q000201:enzymology; D013045:Species Specificity; D013211:Staphylococcus aureus / Q000201:enzymology
D002308:Cardiolipins; D010712:Phosphatidic Acids; D010715:Phosphatidylglycerols; D010740:Phospholipases; D008274:Magnesium