Preparation of prereduced anaerobically sterilized media and their use in cultivation of anaerobic bacteria.
Several modifications of the roll-tube method have made it simpler for routine use in the isolation and growth of anaerobic bacteria. These include use of a check valve for the production of prereduced anaerobically sterilized media; a Salvarsan tube under oxygen-free gas pressure for the dispensing of molten prereduced anaerobically sterilized agar medium; a Kelly infusion bottle with a graduated pipette side arm (also under gas pressure) for quantitative delivery of fluid prereduced anaerobically sterilized media; and screw-capped prescription bottles for the cultivation of anaerobes. Colonies of Bacteroides melaninogenicus were easily identified and counted by this method. Other anaerobic bacteria have also been cultivated successfully.
D000693:Anaerobiosis; D001431:Bacteriological Techniques / Q000295:instrumentation; D001439:Bacteroides / Q000254:growth & development*; D003470:Culture Media; D003773:Dental Plaque / Q000382:microbiology*; D006801:Humans; D001443:Prevotella melaninogenica / Q000254:growth & development*; D013242:Sterilization
D003470:Culture Media