Adenosine triphosphate: nicotinamide mononucleotide adenylyltransferase of pig liver. Purification and properties.
Adenosine triphosphate : nicotinamide mononucleotide adenylyltransferase (EC 2.7.7.1) has been purifiec approximately 3500-fold from an extract of pig liver nuclei to a specific activity of 40 mumol of NAD+ per min per mg protein. The enzyme was found to have a molecular weight of 203 000, a frictional ratio of 1.6 and an isoelectric point of approximately 5. Michaelis constants for ATP and NMN were 0.11 mM and 0.12 mM, respectively.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002850:Chromatography, Gel; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008970:Molecular Weight; D009537:Nicotinamide Mononucleotide / Q000378:metabolism; D009713:Nucleotidyltransferases / Q000302:isolation & purification / Q000378:metabolism*; D009994:Osmolar Concentration; D012965:Sodium Chloride / Q000494:pharmacology; D013552:Swine
D009537:Nicotinamide Mononucleotide; D012965:Sodium Chloride; D000255:Adenosine Triphosphate; D009713:Nucleotidyltransferases