Isolation and preliminary characterization of temperature-sensitive mutants of pseudorabies virus.
Nine ts mutants of pseudorabies virus (PRV) strain BUK were isolated following bromodeoxyuridine (BUdR) or NaNO2 mutagenesis by a selection technique based on a shiftdown from nonpermissive to permissive temperature. This technique is described and its advantages are discussed. The nine ts mutants have been assigned to four complementation groups.
D000818:Animals; D001973:Bromodeoxyuridine; D002642:Chick Embryo; D046508:Culture Techniques; D005816:Genetic Complementation Test; D006564:Herpesviridae / Q000235:genetics*; D011558:Herpesvirus 1, Suid / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification; D009153:Mutagens; D009154:Mutation; D009573:Nitrites; D012641:Selection, Genetic; D013696:Temperature; D014779:Virus Replication
D009153:Mutagens; D009573:Nitrites; D001973:Bromodeoxyuridine