Production of IgG antibodies and enhanced response of nude mice to DNP-AE-dextran.
DNP-AE-dextran, prepared by the binding of DNP-epsilon-aminocaproyl residues to animoethylated dextran (predominantly alpha-1,6-linked), induced a T-independent anti-DNP antibody response in mice. However, certain differences were observed between the response to this antigen in normal andnude mice. Thus, the antibody titers of nu/nu mice from day 10 to 38 after immunization were significantly higher than those of nu/+ controls. Furthermore, DNP-AE-dextran induced a weak secondary response in nu/+ but not in nu/nu mice. For both thymusless and normal mice the production of IgG in addition to IgM antibodies to DNP-AE-dextran could be established. The former included antibodies of the IgG1 subclass which were considered to be particularly thymus dependent (1). The higher response of nu/nu mice was reflected mainly in the increased production of IgG antibodies. Under the influence of a graft-vs-host reaction, a 10-fold increase in antibody titers to DNP-AE-dextran was observed, due entirely to an enhanced IgG response.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D000917:Antibody Formation; D003911:Dextrans / Q000031:analogs & derivatives* / Q000494:pharmacology; D004140:Dinitrophenols / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006087:Graft vs Host Reaction; D006462:Hemolytic Plaque Technique; D007117:Immunization, Secondary; D007074:Immunoglobulin G; D008297:Male; D051379:Mice; D008819:Mice, Nude
D000906:Antibodies; D003911:Dextrans; D004140:Dinitrophenols; D007074:Immunoglobulin G