[Hydroxylation in isolated liver cells].
Ethylenediamine tetraacetate and mechanical treatment were applied to obtain the isolated hepatocytes. Oxidative phosphorylation was found to be preserved in the isolated cells. Hepatocytes were capable of hydroxylation of dimethylaniline (DMA), ethylmorphine and aminopyrine. VMAX for the hydroxylation of DMA calculated as per 1 nmol of the cytochrome P--450 was higher in the cells than in the microsomes. NADH formed during the oxidation of glutamate and malate can be used for hydroxylation.
D000632:Aminopyrine / Q000378:metabolism; D000814:Aniline Compounds / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D002952:Citric Acid Cycle; D006900:Hydroxylation; D066298:In Vitro Techniques; D008099:Liver / Q000166:cytology / Q000201:enzymology*; D008862:Microsomes, Liver / Q000201:enzymology; D008930:Mitochondria, Liver / Q000201:enzymology; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D009022:Morphine Derivatives / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010085:Oxidative Phosphorylation; D051381:Rats
D000814:Aniline Compounds; D009022:Morphine Derivatives; D000632:Aminopyrine; D009243:NAD; D009249:NADP; D006899:Mixed Function Oxygenases
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