The kinetics of the recombination reaction between apomyoglobin and alkaline haematin.
Apomyoglobin was prepared by an extremely mild modification of the acid/butanone technique, and the kinetics of the recombination reaction between this preparation and alkaline haematin were studied. The recombination has been shown to be precisely second-order and mono-phasic. Rate constants obtained from the study are in good agreement with values obtained previously by an indirect technique not involving separation of haem and apoprotein.
D001059:Apoproteins; D006418:Heme / Q000031:analogs & derivatives; D006427:Hemin; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009211:Myoglobin; D013053:Spectrophotometry
D001059:Apoproteins; D009211:Myoglobin; D006418:Heme; D006427:Hemin