[Experimental study on prediction of drug toxicity. Drug-induced hemolysis and lysosomes lysis in vitro].
The effect of drugs on isolated rat liver lysosomes, erythrocytes and some erythrocyte enzymes was studied in vitro. Many tranquilizers, antihistaminics and antidepressants, which are known to have the greatest number of adverse reactions in clinical use, had a lytic effect on the particle membranes at concentrations above 2 X 10(-4) M. The present study revealed a good correlation between the p.o. LD50 (rat and mouse) and H30 (molar concentration of drug causing 30% hemolysis). It is suggested, therefore, that the labilizing effect of drugs on rat erythrocytes is useful for testing the lytic action of drugs in vivo.
D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors; D000818:Animals; D000928:Antidepressive Agents / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000201:enzymology; D005952:Glucose-6-Phosphatase / Q000037:antagonists & inhibitors; D006461:Hemolysis / Q000187:drug effects*; D006634:Histamine H1 Antagonists / Q000633:toxicity*; D066298:In Vitro Techniques; D007928:Lethal Dose 50; D008247:Lysosomes / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D051379:Mice; D051381:Rats; D014149:Tranquilizing Agents / Q000633:toxicity*
D000928:Antidepressive Agents; D006634:Histamine H1 Antagonists; D014149:Tranquilizing Agents; D005952:Glucose-6-Phosphatase; D000251:Adenosine Triphosphatases