Base specificity of polyamine binding to synthetic polynucleotides.
The binding of polyamines and magnesium to synthetic polynucleotides has been studied by gel filtration on a Sephadex G-50 column. Among the single-stranded polynucleotides examined [poly(A), poly(C), and poly(U)], polyamines were found to bind to poly(C) and poly(U) preferentially, while the binding of Mg2+ was greatest with poly(A). Spermine bound to poly(U) was displaced completely by NH4+ but incompletely by Mg2+, while Mg2+ bound to poly(A) was displaced completely be spermine but incompletely by NH4+. The optimal pH for the binding of spermine to poly(U) was found to be about 7.9, while Mg2+ could bind to poly(A) over a broad pH range (7.1--8.7).
D055598:Chemical Phenomena; D002621:Chemistry; D004247:DNA; D004996:Ethidium; D006863:Hydrogen-Ion Concentration; D008274:Magnesium; D011061:Poly A; D011066:Poly C; D011068:Poly G; D011072:Poly U; D011131:Polyribonucleotides / Q000378:metabolism; D000644:Quaternary Ammonium Compounds; D013095:Spermidine / Q000378:metabolism; D013096:Spermine / Q000378:metabolism
D011131:Polyribonucleotides; D000644:Quaternary Ammonium Compounds; D011061:Poly A; D011068:Poly G; D011072:Poly U; D013096:Spermine; D011066:Poly C; D004247:DNA; D004996:Ethidium; D008274:Magnesium; D013095:Spermidine