HCO-3-activated adenosine triphosphatase in intestinal mucosa of the eel.
An HCO-3-activated and SCN--inhibited ATPase (ATP phosphohydrolase, EC 3.6.1.3) found in homogenates of intestinal mucosa of the eel was solubilized by Triton X-100. Optimal HCO-3-concentration and pH for the enzyme were 25 mM and 8.7, respectively. HCO-3-ATPase activity in both homogenate and solubilized preparations increased after seawater adaptation. This adaptive increase in enzyme activity was also observed in the gills and the kidney. The HCO-3-ATPase seems to be related to transport mechanisms, especially for Cl-, in osmoregulatory surfaces of the eel.
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000811:Anguilla; D000818:Animals; D001639:Bicarbonates / Q000494:pharmacology*; D004789:Enzyme Activation; D005753:Gastric Mucosa / Q000201:enzymology; D005880:Gills / Q000201:enzymology; D006863:Hydrogen-Ion Concentration; D007413:Intestinal Mucosa / Q000201:enzymology*; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D008274:Magnesium / Q000494:pharmacology; D009928:Organ Specificity; D011092:Polyethylene Glycols / Q000494:pharmacology; D013861:Thiocyanates / Q000494:pharmacology
D001639:Bicarbonates; D013861:Thiocyanates; D011092:Polyethylene Glycols; D000251:Adenosine Triphosphatases; D008274:Magnesium