Semiautomated spectrophotometry of total phospholipids in plasma.
A one-stage semiautomated method for estimating total phospholipids in plasma is reported. The phospholipids are extracted into aqueous ethanol, combined with a Lewis acid, and the change in pH is monitored colorimetrically. The method, compared with the acid digestion method of Gomori [J. Lab. Clin. Med. 27, 955 (1942)], gives results that are about 0.25 mmol/liter lower, but seems to be a suitable and less hazardous alternative.
D000293:Adolescent; D000328:Adult; D001322:Autoanalysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008875:Middle Aged; D010713:Phosphatidylcholines / Q000097:blood; D010743:Phospholipids / Q000097:blood*; D013053:Spectrophotometry / Q000379:methods
D010713:Phosphatidylcholines; D010743:Phospholipids