Hepatotoxic effect of thioacetamide (TAM) on NADP-linked enzymes, aminotransferases and glutamate dehydrogenase.
NADP-linked dehydrogenases, glucose-6-P dehydrogenase (G 6PDH) 6-P gluconate dehydrogenase (6 PGDH), isocitrate dehydrogenase (ICDH), malate dehydrogenase decarboxylating (ME) and aminotransferases GOT and GPT were analyzed in the soluble fraction of blood free homogenates. Glutmate dehydrogenase (GDH) was assayed in the mitochondrial fraction. TAM was i.p. administered to male albino rats (50 mg/kg/day) for 28 days. enzyme activities were determined as described by Bermeyer 1965 (Methods Enzymatic Analysis. Verlag Chemie. Acad. Press).
D000081:Acetamides / Q000009:adverse effects*; D000410:Alanine Transaminase / Q000378:metabolism; D000429:Alcohol Oxidoreductases / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury / Q000201:enzymology* / Q000209:etiology; D005969:Glutamate Dehydrogenase / Q000378:metabolism*; D008297:Male; D009249:NADP / Q000378:metabolism*; D051381:Rats; D013853:Thioacetamide / Q000009:adverse effects*; D000637:Transaminases / Q000378:metabolism*
D000081:Acetamides; D013853:Thioacetamide; D009249:NADP; D000429:Alcohol Oxidoreductases; D005969:Glutamate Dehydrogenase; D000637:Transaminases; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase