Interaction of colchicine with DNA molecules.
Colchicine (7.5 X 10-6M or 3.3 X 10-5M) was incubated at pH 7.0, 10.0, or 12.0 with high-molecular DNA from salmon sperm (7 X 10-5M or 3.5 X 10-5M DNA-P) at 40 degrees C. Interaction was monitored by UV spectrophotometry; Amax/Amin ratios, difference and additive spectra, and the quantity of colchicine bound to DNA were presented as functions of time, ionic strength and DNA concentration. Using NMR techniques, delta deltav 1/2/deltac values, chemical shifts and half-line widths of colchicine protons were analyzed as a function of the DNA/colchicine ratio, thus proving the interaction between alkaloid and DNA. An intercalation of the tropolone moiety of colchicine between the nitrogenous bases of DNA is suggested.
D000818:Animals; D003078:Colchicine; D004247:DNA; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008297:Male; D012474:Salmon; D013056:Spectrophotometry, Ultraviolet; D013094:Spermatozoa
D004247:DNA; D003078:Colchicine