Indole to phenolate energy transfer, the alkaline quenching mechanism of beta-trypsin fluorescence.
Evidence that indole to phenolate energy transfer causes the decrease in beta-trypsin fluorescence with increasing alkalinity is obtained indirectly from viscosity, lifetime, wavelength dependence and chemical modification studies which either exclude or minimize the involvement of other possible mechanisms.
D004735:Energy Transfer; D006863:Hydrogen-Ion Concentration; D007211:Indoles; D010636:Phenols; D013050:Spectrometry, Fluorescence; D014357:Trypsin; D014443:Tyrosine
D007211:Indoles; D010636:Phenols; D014443:Tyrosine; D014357:Trypsin