Kinetics and properties of L-glutaminase and L-asparaginase activities of Pseudomonas ovalis.
Pseudomonas ovalis produces L-glutaminase and L-asparaginase activities simultaneously upon induction by L-glutamine or L-asparagin in the growth medium. Both activities are confined to the cell during active growth and are not released into the medium. The apparent Km values are 1.4 X 10(-2) M and 6 X 10(-3) M for L-glutamine and L-asparagine substrates, respectively. Induction of both activities is substantially favoured in media with initial pH values higher than 7. In buffered yeast extract L-asparagine medium, significant amounts of L-glutaminase and L-asparaginase activities appeared towards the end of the exponential phase and along the stationary phase. The process of enzyme formation showed a firm link to the cell active growth, as evidenced by the use of growth inhibitors.
D001215:Asparaginase / Q000096:biosynthesis*; D001216:Asparagine / Q000494:pharmacology; D004790:Enzyme Induction; D005972:Glutaminase / Q000096:biosynthesis*; D005973:Glutamine / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D011549:Pseudomonas / Q000187:drug effects / Q000201:enzymology*; D013307:Streptomycin / Q000494:pharmacology; D013754:Tetracyclines / Q000494:pharmacology; D013997:Time Factors
D013754:Tetracyclines; D005973:Glutamine; D001216:Asparagine; D001215:Asparaginase; D005972:Glutaminase; D013307:Streptomycin