[Nad-dependent formate dehydrogenase from methylotrophic bacteria. Characteristics and stability of soluble and immobilized enzyme].
pH-dependency is studied of kinetic parameters of the reaction catalyzed by NAD-dependent formate dehydrogenase from methylotrophic Bacterium spl strain. Values of Km for NAD and formate, and also of maximum reaction rate are found not to change within the pH range from 6 to 9. Role of SH-groups in the development of the enzyme catalytic activity and the effect of different factors on stability of soluble and immobilized enzyme forms are investigated. Molecular weight of the enzyme (70000), extinction coefficient and catalytical constant (6 s-1) are determined.
D000445:Aldehyde Oxidoreductases / Q000378:metabolism*; D001419:Bacteria / Q000201:enzymology*; D004355:Drug Stability; D004800:Enzymes, Immobilized; D005561:Formates; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008970:Molecular Weight; D012995:Solubility; D013438:Sulfhydryl Compounds / Q000378:metabolism
D004800:Enzymes, Immobilized; D005561:Formates; D013438:Sulfhydryl Compounds; D000445:Aldehyde Oxidoreductases
NAD-zavisimaia formiatdegidrogenaza iz metilotrofnykh bakteriĭ. Izuchenie svoĭstv i stabil'nosti rastvorimogo i immobilizovannogo fermenta.