Influence of mild acid hydrolysis on the antigenic properties of phase I Coxiella burnetii.
Mild acid hydrolysis of phase I Coxiella burnetii cells gradually cleaved off polysaccharide chains, which contain determinants of antigen 1, thus unmasking determinants of the diagnostically important antigen 2. This artificial phase I to phase II conversion was reflected by changed serological activity in the complement-fixation (CF) test and by changed nonspecific phagocytosis. The product of mild acid hydrolysis can serve as a complex diagnostic preparation, because it reacts in the CF test with antibodies directed to both C. burnetii antigens.
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D000942:Antigens, Bacterial; D002417:Cattle; D003168:Complement Fixation Tests; D003381:Coxiella / Q000187:drug effects* / Q000276:immunology; D006224:Cricetinae; D006168:Guinea Pigs; D006801:Humans; D006851:Hydrochloric Acid / Q000494:pharmacology*; D006868:Hydrolysis; D008070:Lipopolysaccharides / Q000276:immunology; D051379:Mice; D010587:Phagocytosis; D011135:Polysaccharides, Bacterial / Q000276:immunology; D011778:Q Fever / Q000276:immunology; D012756:Sheep
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D006851:Hydrochloric Acid