The inhibition by 6-diazo-5-oxo-l-norleucine of glutamine catabolism of the cultured human lymphoblast.
The rapid catabolism of glutamine by the cultured human lymphoblast line WI-L2 can be inhibited greater than 95% by incubation of cell suspensions with 6-diazo-5-oxo-L-norleucine (DON). The inhibition persists for at least four hours after removal of DON from the cell suspension. The exposure of cells to DON ihibits over 95% of the glutaminase activity measured in lysates in the presence of either phosphate or maleate. Similarly, gamma-glutamyl transpeptidase, assayed with gamma-glutamyl-p-nitroanilide as substrate and glycyglycine as acceptor, is inhibited over 90%. DON-treated and control cells accumulated radioactive material from suspensions containing [14C]-L-glutamine at similar initial rates; the radioactive material accumulated by the DON-treated cells is all recoverable as glutamine while the radioactive material accumulated by untreated cells is principally recovered as glutamate.
D001377:Azaserine / Q000494:pharmacology; D001391:Azo Compounds / Q000494:pharmacology*; D002460:Cell Line; D003980:Diazooxonorleucine / Q000494:pharmacology*; D005972:Glutaminase / Q000037:antagonists & inhibitors; D005973:Glutamine / Q000378:metabolism*; D012402:Rotenone / Q000494:pharmacology; D005723:gamma-Glutamyltransferase / Q000037:antagonists & inhibitors
D001391:Azo Compounds; D003980:Diazooxonorleucine; D012402:Rotenone; D005973:Glutamine; D001377:Azaserine; D005723:gamma-Glutamyltransferase; D005972:Glutaminase