Stimulation of 2-deoxy-d-glucose transport in control and virus-transformed cells by ethidium bromide.
Recently we demonstrated that ethidium bromide altered the plasma and subcellular membrane glycoproteins in control and virus transformed cells. It is reported here that ethidium bromide also stimulated the membrane associated process of sugar transport. The KM of the virus transformed cells and the ethidium bromide treated cells is the same as that of the control cells while the maximum velocity as compared to the control cells is significantly increased. The transport of 2-deoxyl-D-glucose was inhibited by glucose, cytochalasin B and neuraminidase but was unaffected by variations in cell density or pH of the incubation medium.
D001358:Avian Sarcoma Viruses; D001693:Biological Transport, Active / Q000187:drug effects; D002452:Cell Count; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D003571:Cytochalasin B / Q000494:pharmacology; D003837:Deoxy Sugars / Q000378:metabolism*; D003847:Deoxyglucose / Q000378:metabolism*; D004996:Ethidium / Q000494:pharmacology*; D005947:Glucose / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009439:Neuraminidase / Q000494:pharmacology; D013268:Stimulation, Chemical; D013696:Temperature
D003837:Deoxy Sugars; D003571:Cytochalasin B; D003847:Deoxyglucose; D009439:Neuraminidase; D004996:Ethidium; D005947:Glucose