Adrenergic, cholinergic, and inactive human neuroblastoma cell lines with the action-potential Na+ ionophore.
Cultured human neuroblastoma cell lines were assayed for biochemical characteristics of neuonal function. Cell lines studied included LA-N-1, LA-N-2, IMR-32, SK-N-SH, and SK-N-MC. Veratridine-dependent uptake of 22Na+ implied the presence of the action potential Na+ ionophore in LA-N-1, LA-N-2, IMR-32, and SK-N-SH. The time course of 22Na+ uptake and inhibition of uptake by tetrodotoxin supported this. SK-N-MC had no veratridine-dependent 22Na+ uptake. Tyrosine hydroxylase (EC 1.14.10.), glutamic acid decarboxylase (EC 4.1.1.15), and acetylcholine contents in neuroblastoma cells were compared to those in brain. LA-N-1 and IMR-32 contained 15 and 5 times as much tyrosine hydroxylase, respectively, whereas LA-N-2, SK-N-SH, and SK-N-MC contained only 0.5 to 5% of that in brain. Acetylcholine was present in -LA-N-2 in 15- to 20-fold greater quantities than in brain; other lines had only 10 to 50% of that in brain. None of the cell lines contained glutamic acid decarboxylase. Thus, continuously propogated human neuroblastoma cell lines may have the action potential Na+ ionophore and may be adrenergic (LA-N-1 and IMR-32), cholinergic (LA-N-2), or inactive (SK-N-SH and SK-N-MC). This is the first demonstration of the action potential Na+ ionophore and of acetylcholine production in human neuroblastoma cell lines.
D000109:Acetylcholine / Q000378:metabolism*; D000200:Action Potentials; D002460:Cell Line; D005968:Glutamate Decarboxylase / Q000378:metabolism; D006801:Humans; D007476:Ionophores; D009362:Neoplasm Metastasis; D009447:Neuroblastoma / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*; D013566:Sympathomimetics / Q000378:metabolism*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism; D014702:Veratrine / Q000378:metabolism
D007476:Ionophores; D013566:Sympathomimetics; D012964:Sodium; D014446:Tyrosine 3-Monooxygenase; D005968:Glutamate Decarboxylase; D014702:Veratrine; D000109:Acetylcholine