Biological activity of dilute isoproterenol solution stored for long periods in plastic bags.
The effect of long-term storage in polyvinyl chloride bags on the biological activity and the chemical stability of isoproterenol was studied. A freshly prepared solution of isoproterenol, isoproterenol solution stored for six months and isoproterenol stored for 30 months were infused into the coronary circulation of 14 isolated rabbit hearts; heart rates were determined. Spectrophotometric studies to determine stability at various pH levels were also done. Aging of the solutions at pH 4 and at a concentration of 4 microgram/ml caused no significant impairment of their potency. Solutions above pH 6 were unstable.
D000818:Animals; D004349:Drug Packaging; D004355:Drug Stability; D004356:Drug Storage; D005260:Female; D006339:Heart Rate / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007545:Isoproterenol / Q000032:analysis / Q000494:pharmacology*; D008297:Male; D011143:Polyvinyl Chloride; D011145:Polyvinyls; D011817:Rabbits; D012996:Solutions / Q000032:analysis; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors
D011145:Polyvinyls; D012996:Solutions; D011143:Polyvinyl Chloride; D007545:Isoproterenol