[Effects of nucleophils on kinetic parameters of peroxidase-catalyzed oxidative reactions].
The effect of imidazole on the kinetic parameters of reactions of potassium ferrocyanide and o-dianizidine peroxidation by hydrogen peroxide within a wide range of pH was studied. It was shown that imidazole activates the reaction of o-dianizidine peroxidation at pH greater than or equal to 6.5, but does not affect the oxidation of potassium ferrocyanide. It was also found that imidazole causes a similar increase in the kkat and Km, i. e. non-competitive activation occurs. The data obtained suggest a possible mechanism of the activator effect. Differences in the mechanism of interaction of various substrates uith peroxidase are discussed.
D003962:Dianisidine / Q000378:metabolism; D004789:Enzyme Activation; D005295:Ferrocyanides / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007093:Imidazoles / Q000494:pharmacology*; D007700:Kinetics; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000378:metabolism*; D013379:Substrate Specificity
D005295:Ferrocyanides; D007093:Imidazoles; D010544:Peroxidases; D003962:Dianisidine
Vliianie nukleofilov na kinetiku reaktsii okisleniia, kataliziruemykh peroksidazoĭ.