Simultaneous determination of 5'-nucleotidase and alkaline phosphatase activities in serum.
A simple method is described for the simultaneous determination of alkaline phosphatase (EC 3.1.3.1) and 5'-nucleotidase (EC 3.1.3.5) in serum. The method is based on the determination of inorganic phosphorus released by the action of the two enzymes on adenosine-5'-monophosphate at pH 9.5 (200 mmol/l tris-buffer) in the presence and absence of L-cysteine. This amino acid at a concentration of 2--10 mmol/l was found to be a specific inhibitor for alkaline phosphatase but with no effect on 5'-nucleotidase activity.
D000249:Adenosine Monophosphate; D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors / Q000097:blood*; D000818:Animals; D003486:Cyanides / Q000494:pharmacology; D003545:Cysteine / Q000494:pharmacology; D004789:Enzyme Activation; D005459:Fluorides / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007422:Intestines / Q000201:enzymology; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008722:Methods; D051379:Mice; D009532:Nickel / Q000494:pharmacology; D009708:Nucleotidases / Q000097:blood*
D003486:Cyanides; D000249:Adenosine Monophosphate; D009532:Nickel; D009708:Nucleotidases; D000469:Alkaline Phosphatase; D003545:Cysteine; D005459:Fluorides