Purification of canine prolactin by preparative isotachophoresis.
Preparative isotachophoresis in polyacrylamide gel using carrier ampholytes as 'spacers' has been used to purify prolactin from canine pituitary extracts. Using Ampholine pH 5-8 as spacers, a prolactin fraction was obtained which was essentially homogeneous as judged by the criteria of disc electrophoresis and isoelectric focusing. Amino acid analysis indicated a close similarity between canine and ovine prolactin. A growth hormone fraction, identified by its electrophoretic mobility, was also obtained although this was heterogeneous in disc electrophoresis at alkaline pH.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D004285:Dogs; D004589:Electrophoresis, Disc; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D010902:Pituitary Gland / Q000032:analysis; D011388:Prolactin / Q000032:analysis / Q000302:isolation & purification*
D000596:Amino Acids; D011388:Prolactin