Gel filtration of hepatitis B surface antigen: increased size of the native particle.
The Stokes radius of umpurified hepatitis B antigen (HBSAg) was determined by chromatography on a carefully calibrated Sepharose 4B column. A value of 14-2 nm was found by this procedure, contrasting with a published value of II nm for purified, pepsin-treated HBSAg. Chromatography at pH 3 appeared to reduce the Strokes radius of HBSAg to II nm. Evidence is presented to show that serum proteins adsorbed to HBSAg can be removed with pepsin or acid.
D001798:Blood Proteins / Q000302:isolation & purification; D002850:Chromatography, Gel; D006514:Hepatitis B Surface Antigens / Q000302:isolation & purification; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D010434:Pepsin A / Q000378:metabolism
D001798:Blood Proteins; D006514:Hepatitis B Surface Antigens; D010434:Pepsin A