Changes in the electric dipole vector of human serum albumin due to complexing with fatty acids.
The magnitude of the electric dipole vector of human serum albumin, as measured by the dielectric increment of the isoionic solution, is found to be a sensitive, monotonic indicator of the number of moles (up to at least 5) of long chain fatty acid complexed. The sensitivity is about three times as great as it is in bovine albumin. New methods of analysis of the frequency dispersion of the dielectric constant were developed to ascertain if molecular shape changes also accompany the complexing with fatty acid. Direct two-component rotary diffusion constant analysis is found to be too strongly affected by cross modulation between small systematic errors and physically significant data components to be a reliable measure of structural modification. Multicomponent relaxation profiles are more useful as recognition patterns for structural comparisons, but the equations involved are ill-conditioned and solutions based on standard least-squares regression contain mathematical artifacts which mask the physically significant spectrum. By constraining the solution to non-negative coefficients, the magnitude of the artifacts is reduced to well below the magnitudes of the spectral components. Profiles calculated in this way show no evidence of significant dipole direction or molecular shape change as the albumin is complexed with 1 mol of fatty acid. In these experiments albumin was defatted by incubation with adipose tissue at physiological pH, which avoids passing the protein through the pH of the N-F transition usually required in defatting. Addition of fatty acid from soluion in small amounts of ethanol appears to form a complex indistinguishable from the "native" complex.
D001665:Binding Sites; D004563:Electrochemistry; D005227:Fatty Acids; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008433:Mathematics; D009829:Oleic Acids; D009994:Osmolar Concentration; D011485:Protein Binding; D012709:Serum Albumin; D012710:Serum Albumin, Bovine
D005227:Fatty Acids; D009829:Oleic Acids; D012709:Serum Albumin; D012710:Serum Albumin, Bovine