Separation of pesticide residues from lipids prior to gas-liquid chromatographic analysis.
Lipids are separated from dieldrin, endrin, and p,p'-DDE residues by saponification in ethanolic sodium hydroxide, acidification with dilute sulfuric acid, and adsorption chromatography on deactivated alumina, using petroleum ether as the eluant. Dieldrin, endrin, and p,p'-DDE are efficiently recovered (95-102%), and p,p'-DDT is converted to p,p'-DDE, which is then recovered with high yield (90-96%). Extremely low lipid carryover (less than 0.3-0.5%) is observed for 0.5-1.0 g samples of chicken fat.
D000327:Adsorption; D000535:Aluminum; D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas; D003633:Dichlorodiphenyl Dichloroethylene / Q000032:analysis; D004026:Dieldrin / Q000032:analysis; D004732:Endrin / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D007306:Insecticides / Q000032:analysis*; D008055:Lipids / Q000032:analysis*; D010087:Oxides; D010573:Pesticide Residues / Q000032:analysis*; D012997:Solvents
D007306:Insecticides; D008055:Lipids; D010087:Oxides; D010573:Pesticide Residues; D012997:Solvents; D003633:Dichlorodiphenyl Dichloroethylene; D000535:Aluminum; D004026:Dieldrin; D004732:Endrin