[Guinea pig liver cytosol has an L-threonine deaminase activity distinct from L-serine deaminase].
Using sodium sulfate precipitation, "Sephadex G200" gel filtration and polyacrylamide gel electrophoresis, a L-threonine desaminase was demonstrated in the Guinea-Pig liver cytosol. This enzyme was separated from the guinea pig liver L-serine desaminase possessing an auxiliary activity on L-threonine substrate described by us in a previous work. The optimals for pH (7,1) and temperature (+ 55 degrees C) and the apparent molecular weight (134,000 + 20,000) were established.
D000818:Animals; D003600:Cytosol / Q000201:enzymology; D006168:Guinea Pigs; D006836:Hydro-Lyases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D012695:L-Serine Dehydratase / Q000302:isolation & purification / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D008970:Molecular Weight; D013913:Threonine Dehydratase / Q000378:metabolism*
D006836:Hydro-Lyases; D012695:L-Serine Dehydratase; D013913:Threonine Dehydratase
Mise en évidence dans le cytosol du foie de cobaye d'une activité L-thréonine désaminasique distinct de la L-sérine désaminase