Purification of alpha-L-fucosidase from various sources by affinity chromatography.
An affinity column for alpha-L-fucosidases was constructed by linking p-amino-phenyl 1-thio-alpha-L-fucopyranoside to Sepharose 4B through linkers of succinyl 3,3'-diamino-dipropylamine. Excellent purification of alpha-L-fucosidase from rat epididymis, Clostridium perfringens and Limulus polyphemus (horse shoecrab) could be effected inone step with good yield. An affinity column purification step can be introduced at any point in published purification procedures. The purified enzyme is essentially free of other glycosidases and proteolytic enzymes. The column material is stable and can be reused for at least two years.
D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods; D003016:Clostridium perfringens / Q000201:enzymology; D004186:Disaccharidases / Q000302:isolation & purification*; D004822:Epididymis / Q000201:enzymology; D006737:Horseshoe Crabs / Q000201:enzymology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008297:Male; D011714:Pyrans / Q000138:chemical synthesis; D051381:Rats; D005644:alpha-L-Fucosidase / Q000302:isolation & purification*
D011714:Pyrans; D004186:Disaccharidases; D005644:alpha-L-Fucosidase