An immunoprecipitation-dissociation technique for large scale antibody purification and an antigen consumption electroimmunoassay for antibody quantitation. A model study with antibodies to pregnancy zone protein.
A simple immunoprecipitation--dissociation technique for large scale purification of antibodies is described, which comprises selective denaturation of the antigen and recovery of the antibody fraction by exclusion chromatography at low pH. Its use is illustrated by the purification of antibodies to pregnancy zone protein. A purification factor of about 60 was achieved. An antigen consumption electroimmunoassay was also developed which permits quantitative determination of the antigen binding activity of antibodies with a given specificity. The methods have general application.
D000906:Antibodies / Q000302:isolation & purification*; D001798:Blood Proteins / Q000276:immunology*; D004563:Electrochemistry; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007118:Immunoassay; D007163:Immunosorbent Techniques; D011247:Pregnancy; D011263:Pregnancy Trimester, Third
D000906:Antibodies; D001798:Blood Proteins