[Conditions for splitting protodioscine--the main glycoside from Tribulus terrestris L. by the enzymatic preparation from Aspergillus niger BKMt-33].
The conditions for splitting protodioscine--the main steroid saponine isolated from Tribulus terrestris L. by the enzymic preparation of Aspergillus niger str. BKMt-33 were investigated. The optimal conditions were found to be as follows: pH 4-5, temperature 30-37 degrees (the substrate concentration--5 mg%, concentration of the enzymic preparation--1%). Under these conditions the enzymolysis continued 24 hours. Mg+2 and K+ ions accelerated the reaction twice. As a result of the enzymic hydrolysis dioscine and trilline were obtained. This indicates beta-glucosidase and alpha-rhamnosidase activities of the enzymic complex isolated from Aspergillus niger str. BKMt-33.
D001230:Aspergillus / Q000201:enzymology*; D001234:Aspergillus niger / Q000201:enzymology*; D004789:Enzyme Activation / Q000187:drug effects; D005959:Glucosidases / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006027:Glycosides / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D009097:Multienzyme Complexes / Q000378:metabolism*; D011188:Potassium / Q000494:pharmacology; D013256:Steroids / Q000378:metabolism*; D013696:Temperature
D006027:Glycosides; D009097:Multienzyme Complexes; D013256:Steroids; D005959:Glucosidases; D006026:Glycoside Hydrolases; D008274:Magnesium; D011188:Potassium
Usloviia rasshchepleniia fermentnym preparatom Aspergillus niger BKMt-33 protodiostsina--osnovnogo glikozida iz Tribulus terrestris L