[Optimization of conditions for determining the activity of amphotericin B].
The effect of incubation temperature, pH  and medium composition on the size of the growth inhibitions zones, clearance of their borders and slope of the dose-response curve in determin ation of the biological activity of amphotericin B by the agar-diffusion method using Candida scotti, Tul-1 as the test-microbe was studied. The composition of the medium providing optimal conditions for the activity assay according to the sone size and clearness was developed. When the above medium was used for determination of the biological activity of amphotericin B, the standard deviatioos of the assay results, as well as the average results obtained at an interval of 1 month were 1.5 times lower than with the use of the medium proposed at an interval of 1 month were 1.5 times lower than with the use of the medium proposed in Specification 42 No. 3857-70. At the same time the average results of the assay on both media coincided.
D000666:Amphotericin B / Q000494:pharmacology*; D002175:Candida / Q000187:drug effects / Q000254:growth & development; D003470:Culture Media / Q000494:pharmacology; D004058:Diffusion; D006863:Hydrogen-Ion Concentration; D013696:Temperature; D013997:Time Factors
D003470:Culture Media; D000666:Amphotericin B
Optimizatsiia usloviĭ opredeleniia aktovnosti amfoteritsina B