A rapid method for measuring guanylate cyclase activity in mammary tissue.
A simple and rapid method for measuring guanylate cyclase activity in broken cell preparations of biological tissues is described. This method employs the rate of conversion of [32P]GTP to [32P]cyclic-GMP. The product of this reaction is isolated by ion-exchange chromatography and by a ZnSO4-Ba(OH)2 precipitation at pH 5.7. Using this method, about 30-50 samples can be assayed for guanylate cyclase activity during a 5-6 hr period. The characteristics of this enzyme in the mammary gland were found to be similar to those described for other tissues using different methods for measuring guanylate cyclase activity.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D011232:Chemical Precipitation; D002852:Chromatography, Ion Exchange; D006152:Cyclic GMP / Q000032:analysis; D005260:Female; D005459:Fluorides / Q000494:pharmacology; D006162:Guanylate Cyclase / Q000032:analysis*; D007700:Kinetics; D008297:Male; D008321:Mammary Glands, Animal / Q000201:enzymology*; D008345:Manganese / Q000494:pharmacology; D051379:Mice; D011388:Prolactin / Q000494:pharmacology
D008345:Manganese; D011388:Prolactin; D006162:Guanylate Cyclase; D006152:Cyclic GMP; D005459:Fluorides; D002118:Calcium