[Detection and quantitative estimation of morphine in the urine of drug addicts by combined 2-dimensional thin-layer chromatography and spectrofluorometry].
Following extraction from urine and thin-layer bidimensional chromatography, the suspected spot of morphine is located by UV examination at 350 nm and eluted with methanol by means of "Eluchrom" apparatus. The dried residue is then subjected to spectrofluorimetric analysis, in definite conditions. This procedure can be used to confirm the identification of the alkaloid and to achieve its estimation. The sensitivity and the recoveries of various quantities of morphine added to urine were determined.
D002855:Chromatography, Thin Layer; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008722:Methods; D009020:Morphine / Q000652:urine*; D009021:Morphine Dependence / Q000652:urine*; D013050:Spectrometry, Fluorescence
D009020:Morphine
Détection et estimation quantitative de la morphine dans l'urine des toxicomanes par couplage chromatographie bidimensionnelle sur couche mince--spectrofluorimétrie