Prostaglandin synthesis in human placenta and fetal membranes.
The conversion of exogenous arachidonic acid into prostaglandins was studied in human placenta and fetal membrane microsomes. Only one prostaglandin was formed, prostaglandin E2 (PGE2), in fetal membrane microsomes. In placental microsomes PGE2 was further transformed into 15 keto-PGE2. Cofactor requirements and some characteristics of the system were studied. 1 to 3% conversion of arachidonic acid into prostaglandins was observed in placental microsomes and 5 to 8% conversion in fetal membrane microsomes.
D001095:Arachidonic Acids / Q000378:metabolism; D001894:Borohydrides / Q000494:pharmacology; D002855:Chromatography, Thin Layer; D005321:Extraembryonic Membranes / Q000378:metabolism*; D005260:Female; D006801:Humans; D006851:Hydrochloric Acid / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D006911:Hydroxyprostaglandin Dehydrogenases / Q000494:pharmacology; D008861:Microsomes / Q000378:metabolism; D010084:Oxidation-Reduction; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D011453:Prostaglandins / Q000032:analysis / Q000096:biosynthesis*; D011458:Prostaglandins E / Q000032:analysis / Q000096:biosynthesis; D013997:Time Factors
D001095:Arachidonic Acids; D001894:Borohydrides; D011453:Prostaglandins; D011458:Prostaglandins E; D006911:Hydroxyprostaglandin Dehydrogenases; D011451:Prostaglandin-Endoperoxide Synthases; D006851:Hydrochloric Acid