Immobilized glucuronosyltransferase for the synthesis of conjugates.
Partially purified rabbit liver UDPglucuronosyltransferase is immobilized on agarose by the cyanogen bromide activation method. Both soluble and matrix-bound enzyme preparations display very similar Km and pH optimum. The storage stability of the immobilized enzyme at 4 degrees is 5-10 times improved over the soluble preparations. The agarose-bound UDPglucuronosyltransferase is successfully used in the synthesis of p-nitrophenyl glucuronide in an overall yield of 50-70%. The matrix-bound enzyme is reusable over an extended period of time and offers an easy and convenient synthetic tool for various drug glucuronides.
D005965:Glucuronates / Q000138:chemical synthesis / Q000378:metabolism*; D014453:Glucuronosyltransferase; D006602:Hexosyltransferases; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008566:Membranes / Q000378:metabolism; D009596:Nitrophenols / Q000138:chemical synthesis / Q000378:metabolism; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D012685:Sepharose; D012995:Solubility
D005965:Glucuronates; D009596:Nitrophenols; D004364:Pharmaceutical Preparations; D012685:Sepharose; D006602:Hexosyltransferases; D014453:Glucuronosyltransferase