A chemiluminescent effect encountered in the liquid scintillation counting of 32P phosphate.
During the course of a kinetic enzyme study of bisphosphoglyceromutase (EC 2.7.5.4.) a chemiluminescent effect was encountered in the liquid scintillation counting of the beta-emission from 32Pi. A method is described for overcoming the effect, thereby permitting the accurate determination of the radioactive compounds. It is important to check for chemiluminescent effects early in the development of new procedures that involve liquid scintillation counting.
D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008163:Luminescent Measurements; D010736:Phosphoglycerate Mutase; D010761:Phosphorus Radioisotopes; D012588:Scintillation Counting; D013997:Time Factors
D010761:Phosphorus Radioisotopes; D010736:Phosphoglycerate Mutase