Initial rates of pyruvate transport in mitochondria determined by an "inhibitor-stop" technique.
An "inhibitor-stop" technique has been developed for measuring initial rates of pyruvate transport into mitochondria. The technique uses alpha-cyano-3-hydroxycinnamate as the inhibitor and separates the mitochondria from the radioactive medium by Millipore filtration. Observed rates depend on availability of hydroxyl and other exchangeable anions within the mitochondrial matrix.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002934:Cinnamates / Q000494:pharmacology*; D003373:Coumaric Acids / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D009570:Nitriles / Q000494:pharmacology; D011773:Pyruvates / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D013696:Temperature; D013997:Time Factors
D002934:Cinnamates; D003373:Coumaric Acids; D009570:Nitriles; D011773:Pyruvates