Inhibitory activity of pyridindolol on beta-galactosidase.
The activity of pyridindolol in inhibiting beta-galactosidases obtained from various sources has been studied. Whereas acid bovine liver beta-galactosidase (optimal pH 4.0) was not affected by this compound, neutral bovine liver beta-galactosidase (pH-optimum = 7.0) was inhibited by pyridindolol in reaction mixtures of pH 4.0 approximately 5.0. There was no inhibition at pH 7.0. The type of inhibition is non-competitive by formation of a pyridindolol-enzyme complex. Since beta-galactosidases from other sources are not affected by pyridindolol, the inhibitory action of this compound seems to be rather specific for neutral bovine liver beta-galactosidase.
D000818:Animals; D002243:Carbolines / Q000494:pharmacology*; D002417:Cattle; D002850:Chromatography, Gel; D004347:Drug Interactions; D005696:Galactosidases / Q000037:antagonists & inhibitors*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007211:Indoles / Q000494:pharmacology*; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D013552:Swine
D002243:Carbolines; D007211:Indoles; D005696:Galactosidases