Production and purification of the gamma haemolysin of Staphylococcus aureus 'Smith 5R'.
The gamma haemolysin of Staphylococcus aureus 'Smith 5R' was produced on Dolman-Wilson agar overlain with cellophane. Maximal yields of crude lysin with titres of 2000 to 4000 haemolytic units/ml were obtained within 24 h at 37 degrees C in 10% (v/v) CO2 in air, on medium adjusted to pH 7-0. The crude lysin was purified 2700-fold (with 75% recovery) by ultrafiltration, gel filtration and ammonium sulphate fractionation. The specific activity of the lysin was 10(5) haemolytic units/mg protein after the dialysed active precipitate was extracted with NaCl and reprecipitated with ammonium sulphate. Purified gamma lysin was homogeneous by disc electrophoresis and immunoelectrophoresis.
D000645:Ammonium Sulfate; D002245:Carbon Dioxide / Q000494:pharmacology; D002850:Chromatography, Gel; D003470:Culture Media; D005590:Fractional Precipitation; D006460:Hemolysin Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D013211:Staphylococcus aureus / Q000254:growth & development / Q000378:metabolism*; D013696:Temperature
D003470:Culture Media; D006460:Hemolysin Proteins; D002245:Carbon Dioxide; D000645:Ammonium Sulfate