A quantitative study of leukocyte cohesion: effects of divalent cations and pH.
Leukocyte cell-to-cell adhesion was studied with a cell monolayer technique. The amount of protein retained on the coverslip reflected the number of adhering cells. When polymorphonuclear leukocytes were suspended in a balanced salt solution at neutral pH containing no bivalent cations, they adhered to the glass surface but not to each other. Magnesium or calcium at concentrations above 1 mM caused cell-to-cell adhesion. Stannous and chromate ions had no effect on leukocyte cell-to-cell adhesion. Acid pH also induced leukocyte aggregation. This last effect could be reversed by washing the cells with a balanced salt solution at neutral pH, but it could not be reversed by alcohol.
D002118:Calcium / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002840:Chromates / Q000494:pharmacology; D000431:Ethanol / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007553:Isotope Labeling; D008274:Magnesium / Q000494:pharmacology; D009504:Neutrophils / Q000187:drug effects; D014001:Tin / Q000494:pharmacology
D002840:Chromates; D000431:Ethanol; D014001:Tin; D008274:Magnesium; D002118:Calcium