Properties of hydroxysteroid oxidoreductase isolated from yeast.
Yeasts can advantageously be utilized for the production of the 17beta-hydroxy-derivative, from 3-methoxy-8,14-seco-1,3,5(10),9(11)-estratetraene-14,17-dione (14,17-dione) while 14alpha-hydroxy and 14alpha,17beta-dihydroxy-derivatives are also formed. The biochemical properties of yeasts' enzymes responsible for the formation of the two monohydroxy-derivatives have been studied in detail. In the cell-free extract of Saccharomyces the presence of two hydroxysteroid oxidoreductases could be detected. The first enzyme forms 3beta,17beta-dihydroxy-derivative from 5alpha-androstane-317-dione. This enzyme is responsible for the formation of 17beta-hydroxy-derivative from 14,17-dione. The second enzyme forms 3alpha-hydroxy-derivative from 5beta-androstanedione as well as 14alpha-hydroxy-derivative from its 14,17-dione. The cofactor of both enzymes is pyridine nucleotide. The two enzymes possessing different properties can selectively be inhibited.
D000731:Androstanes / Q000378:metabolism; D002474:Cell-Free System; D055598:Chemical Phenomena; D002621:Chemistry; D004963:Estrenes / Q000378:metabolism; D005285:Fermentation; D006913:Hydroxysteroid Dehydrogenases / Q000378:metabolism; D007664:Ketosteroids / Q000096:biosynthesis; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D009654:Norsteroids / Q000096:biosynthesis; D012440:Saccharomyces / Q000201:enzymology*
D000731:Androstanes; D004963:Estrenes; D007664:Ketosteroids; D009654:Norsteroids; D009243:NAD; D009249:NADP; D006913:Hydroxysteroid Dehydrogenases