Absorption of free and antibody-conjugated horseradish peroxidase to fixed glial cells in tissue culture, a possible source of error in immunohistochemistry.
Incubation of cellular preparations with antibody-conjugated peroxidase result in a very strong unspecific staining, for one electropolar binding of the basic enzymic protein with the cell structures. In immunohistochemical practice, it may represent an evil as import as when dealing with immunofluorescence techniques. This background can to a great extent be avoided by rinsing the preparations in buffer at pH = 6.2.
D000042:Absorption; D001921:Brain / Q000166:cytology; D003951:Diagnostic Errors; D005404:Fixatives; D006735:Horseradish Peroxidase / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007124:Immunoenzyme Techniques; D009457:Neuroglia / Q000378:metabolism*
D005404:Fixatives; D006735:Horseradish Peroxidase