[Regulation of urocaninase activity in the liver: role of 3',5'-AMP].
A dependence of rat liver urocaninase activity on the agents affecting the adenylate cyclase system was studied in vitro and in vivo. Urocaninase is considerably activated after the injection of glucagone, NaF, theophylline and 3',5'-AMP. Under conditions optimal for the protein kinase activity of phosphorylase the urocaninase of liver extracts was activated 7-fold on the average. The nezyme retains its activity after gel-filtration through Sephadex G-25 and is capable of inactivation in the presence of Mg2+ and of reactivation after addition of ATP and 3',5'-AMP. These data suggest a possibility of regulation of mammalian liver urocaninase activity by 3',5'-AMP-dependent phosphorylation of the enzyme. Derivatives of hypoxanthine (theophylline and caffeine) in concentration 10(-4) M activate urocaninase in liver extracts 2--3 and 1.5-fold respectively. The activation is probably not due to the 3',5'-AMP phosphodiesterase inhibition, since another phosphodiesterase inhibitor--papaverine--has no activating effect on urocaninase.
D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism* / Q000494:pharmacology; D005260:Female; D005934:Glucagon / Q000494:pharmacology; D006836:Hydro-Lyases / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008278:Magnesium Sulfate / Q000494:pharmacology; D008297:Male; D011817:Rabbits; D051381:Rats; D012969:Sodium Fluoride / Q000494:pharmacology; D013806:Theophylline / Q000494:pharmacology; D014559:Urocanate Hydratase / Q000378:metabolism*
D008278:Magnesium Sulfate; D012969:Sodium Fluoride; D005934:Glucagon; D013806:Theophylline; D000242:Cyclic AMP; D006836:Hydro-Lyases; D014559:Urocanate Hydratase
Reguliatsiia aktivnosti urokaninazy v pecheni: rol' 3',5'-AMP