Studies on gamma-glutamyl transpeptidase of human urine.
A simple and rapid method suitable for clinical laboratories, for the removal of the inhibitors in urine of gamma-glutamyl transpeptidase activity, using an ion-retardation resin, is described. Gel chromatography of urine resulted in appreciable loss of the enzyme activity. Freezing at -5 degrees C and thawing, did not affect the activity of the enzyme. Dimethyl sulfoxide and polyvinyl pyrrolidone up to a creatinine and NaCl at levels found in normal urine did not inhibit the transpeptidase.
D002712:Chlorides / Q000652:urine; D002852:Chromatography, Ion Exchange; D003404:Creatinine / Q000652:urine; D003956:Dialysis; D005615:Freezing; D006801:Humans; D007700:Kinetics; D008722:Methods; D012964:Sodium / Q000652:urine; D014508:Urea / Q000652:urine; D005723:gamma-Glutamyltransferase / Q000037:antagonists & inhibitors / Q000652:urine*
D002712:Chlorides; D014508:Urea; D012964:Sodium; D003404:Creatinine; D005723:gamma-Glutamyltransferase