Purification and properties of hexoasmine isomerase (EC5.3.1.19) from pig intestinal mucosa.
Hexosamine isomerase from pig intestinal mucosa was purified about 70-fold. The aminosugar product of the enzymatic reaction was identified as glucosamine 6-phosphate and amino acid product as glutamic acid. The pH optimum was 7.1 Km for fructose 6-phosphate was 6.3 X 10(-4) mol/1 and for L-glutamine 6.5 X 10(-4) mol/1.
D000818:Animals; D002238:Carbohydrate Epimerases / Q000378:metabolism*; D005945:Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing) / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000201:enzymology*; D007700:Kinetics; D013552:Swine; D013997:Time Factors
D005945:Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing); D002238:Carbohydrate Epimerases