Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamide.
The thermal stabilities of RNA:DNA hybrids are substantially greater than those of DNA:DNA duplexes in aqueous electrolyte solutions containing high concentrations of formamide. Association rates to form DNA:DNA duplexes and DNA:RNA hybrids have been measured in these solvents. There is a temperature range in which DNA:DNA rates are negligible and RNA:DNA rates close to optimal.
D004273:DNA, Neoplasm; D004355:Drug Stability; D004553:Electric Conductivity; D005559:Formamides; D006367:HeLa Cells; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D012334:RNA, Neoplasm; D012335:RNA, Ribosomal
D004273:DNA, Neoplasm; D005559:Formamides; D012334:RNA, Neoplasm; D012335:RNA, Ribosomal