[Physico-chemical properties of swine vesicular disease virus].
Titration of SVDV on primary pig kidney cell cultures revealed a plating efficiency of less than or equal to 0,9 X 10(-3). Concentration and purification of the SVD-Virus propagated on pig kidney cell cultures were done by chloroform treatment, adsorption, differential- and density gradient centrifugation. The following physical parameters were found: SVDV is an isometrical RNA-virus having a diameter of 25,1 +/- 1,0 nm. It is resistent to the action of chloroform, ether and pH. The virus has a sedimentation coefficient of 156 +/- 3S and a bouyant density in CsCl of 1,33 +/- 0,01 g/ml. Within the family of picornaviruses the SVDV belongs to the subgroup of enteroviruses and can be distinguished from the foot-and-mouth disease virus by the difference in pH-sensitivity and bouyant density in CsCl.
D000818:Animals; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D002725:Chloroform / Q000494:pharmacology; D003588:Cytopathogenic Effect, Viral; D004279:DNA, Viral / Q000145:classification; D004770:Enterovirus / Q000187:drug effects / Q000648:ultrastructure; D004769:Enterovirus Infections; D004986:Ether / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007668:Kidney; D013552:Swine; D013555:Swine Vesicular Disease; D014461:Ultracentrifugation
D004279:DNA, Viral; D004986:Ether; D002725:Chloroform
Einige physikalisch-chemische Eigenschaften des Virus der vesukulären Schweinekrankheit