Cytochrome P-450 and the metabolism of vinyl chloride.
The oxidation of vinyl chloride to non-volatile products is dependent on NADPH and microsomal enzymes. The addition of vinyl chloride to microsomes causes a Type 1 spectra shift, similar to that seen for phenobarbital [11[ which indicates the direct involvement of a cytochrome P-450 species; this difference spectrum is characteristic of substrate binding to this type of enzyme. A glutathione conjugate is probably formed, perhaps via a reactive intermediate.
D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D005978:Glutathione / Q000494:pharmacology; D066298:In Vitro Techniques; D008862:Microsomes, Liver / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction; D051381:Rats; D014752:Vinyl Chloride / Q000378:metabolism*; D014753:Vinyl Compounds / Q000378:metabolism*
D014753:Vinyl Compounds; D009249:NADP; D003577:Cytochrome P-450 Enzyme System; D005978:Glutathione; D014752:Vinyl Chloride