Amino acid sequence of toxin III from Anemonia sulcata.
Toxin III, the smallest toxin component of the poison of the sea anemone Anemonia sulcata, is a polypeptide with 27 amino acids. Its structure is stabilized by three disulfide bridges. The amino acid sequence was determined by solid-phase Edman degradation of the aminoethylated derivative. The peptide was coupled to the carrier, porous glass, by thiourea bridges between the alpha-amino group of arginine-1 and the epsilon-amino group of lysine-26 and the isothiocyanate groups of the carrier. Another fraction of the polypeptide was bound by an acid-amide condensation of the C-terminal valine-27 with the aminopropyl group of the carrier. The sequence of toxin III has no regions homologous to the 47-residue toxin II. Comparison with the known partial sequence of toxin I, which contains 46 amino acids (Wunderer, G. & Eulitz, M., in preparation) also fails to reveal homologies.
D000595:Amino Acid Sequence; D000818:Animals; D003063:Cnidaria / Q000032:analysis*; D003064:Cnidarian Venoms; D004220:Disulfides / Q000032:analysis; D010455:Peptides; D011487:Protein Conformation; D012615:Sea Anemones / Q000032:analysis*
D003064:Cnidarian Venoms; D004220:Disulfides; D010455:Peptides