[Methanol oxidation by the NAD-specific dehydrogenase of methane-assimilating yeasts].
The activity of NAD-specific dehydrogenase (NAD-DH) capable of methanol oxidation was found in cell-free extracts of methanol assimilating yeast cultures Pichia pinus and Candida boidinii. The optimum pH of the incubation mixture for the enzyme is 7.8--7.9; the value of Km of the enzyme for methanol is 1.4-10(-4) M in P. pinus and 1.04-10(-3) M in C. boidinii. NAD-DH is absent from the cells cultivated on glucose, and is induced with growth on a medium containing methanol.
D001203:Ascomycota / Q000201:enzymology*; D002175:Candida / Q000201:enzymology* / Q000378:metabolism; D002384:Catalysis; D002474:Cell-Free System; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D000432:Methanol / Q000378:metabolism*; D010843:Pichia / Q000201:enzymology* / Q000378:metabolism
D000432:Methanol
Okislenie metanola NAD-spetsificheskoĭ degidrogenazoĭ metanolusvaivaiushchikh drozhzheĭ