Lymphoid cell fractionation by aggregated immunoglobulin-agarose columns.
Fractionation by columns of aggregated rat immunoglobulin (Agg Ig)-agarose was investigated as a method of separating different populations of lymphoid cells. With rat spleen cells, Agg Ig columns retained phagocytes, IgM- and IgG-antibody-forming-cells, cells mediating antibody- or PHA-induced lysis of chicken erythrocytes, and specifically immune splenocytes lytic to chicken erythrocytes without exogenous antibody. Agg Ig columns did not selectively remove 'B lymphocytes' (surface-Ig-bearing lymphocytes with or without EAC' receptors), or T lymphocytes capable of PHA-induced proliferation or graft-versus-host reactivity. With mouse spleen cells, Agg Ig columns retained alloimmune cytotoxic T cells.
D000818:Animals; D000921:Antibody-Producing Cells; D002469:Cell Separation / Q000295:instrumentation*; D002645:Chickens; D003601:Cytotoxicity Tests, Immunologic; D004912:Erythrocytes / Q000276:immunology; D006087:Graft vs Host Reaction; D007104:Immune Adherence Reaction; D007136:Immunoglobulins; D008213:Lymphocyte Activation; D008214:Lymphocytes; D051379:Mice; D008810:Mice, Inbred C57BL; D011134:Polysaccharides; D051381:Rats; D011947:Receptors, Antigen, B-Cell; D011955:Receptors, Drug; D012685:Sepharose; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology
D007136:Immunoglobulins; D011134:Polysaccharides; D011947:Receptors, Antigen, B-Cell; D011955:Receptors, Drug; D012685:Sepharose