Studies on the multiple forms of gamma-glutamyltransferase.
In sera of 53 patients with highly raised gamma-glutamyltransferase (gamma-GT) (EC 2.3.2.2) activity, the gamma-GT was separated by column chromatography on Sephadex DEAE A-50 into two fractions. In 35 sera only one gamma-GT fraction was found at a conductivity of 2.0--2.5 mS and in 18 sera two gamma-GT fractions were found at a conductivity of 0.2 mS and again at 2.0--2.5 mS. There was no opvious correlation to the underlying disease. In 20 sera the additional parameters, protein, protein electrophoresis, free and esterified cholesterol, bilirubin, triglycerides, lipoprotein pattern and lipoprotein X were examined. We recognized, that the two forms of the gamma-GT are not true isoenzymes but that the separation of the gamma-GT into two fractions is caused by a different distribution of the cholesterol containing lipoprotei,s.
D001663:Bilirubin / Q000097:blood; D001798:Blood Proteins / Q000378:metabolism; D002784:Cholesterol / Q000097:blood; D002852:Chromatography, Ion Exchange; D002914:Chylomicrons / Q000097:blood; D006801:Humans; D007527:Isoenzymes / Q000097:blood* / Q000302:isolation & purification; D007700:Kinetics; D008074:Lipoproteins / Q000097:blood; D014280:Triglycerides / Q000097:blood; D005723:gamma-Glutamyltransferase / Q000097:blood* / Q000302:isolation & purification
D001798:Blood Proteins; D002914:Chylomicrons; D007527:Isoenzymes; D008074:Lipoproteins; D014280:Triglycerides; D002784:Cholesterol; D005723:gamma-Glutamyltransferase; D001663:Bilirubin