On the pH-dependence of the reaction of hemoglobin with carbon monoxide.
Flash-photolysis experiments were performed on solutions of carbonmonoxy hemoglobin (human Hb A) as function as pH. The fraction of fast reaction and the amount of photodissociation as produced by a given amount of light quanta has been analyzed in terms of the allosteric model of ligand binding by Monod, Wyman and Changeux. It is shown how the switch-over point of the allosteric transition from the T or R state is controlled by the protons, which act as allosteric effectors.
D002248:Carbon Monoxide / Q000097:blood*; D002263:Carboxyhemoglobin; D006441:Hemoglobin A; D006454:Hemoglobins; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008433:Mathematics; D010777:Photochemistry
D006454:Hemoglobins; D002248:Carbon Monoxide; D006441:Hemoglobin A; D002263:Carboxyhemoglobin