High-resolution liquid chromatographic analysis of methylated purine and pyrimidine bases in transfer RNA.
Methylated and major purine and pyrimidine bases were separated and quantified by high-resolution liquid chromatography after hydrolyzing transfer ribonucleic acids (tRNAs). Separation was accomplished by eluting the hydrolyzed samples from an anion-exchange column with a concentration gradient of ammonium acetate at pH 9.2. Isolated sample of tRNA were hydrolyzed to the free bases with a trifluoroacetic acid-formic acid mixture of 200 degrees. Detection limits of 100-200 ng/ml were measured for the methylated bases; analytical data are reported for ten methylated bases plus the four major bases of calf liver and rat liver tRNA.
D000225:Adenine / Q000031:analogs & derivatives* / Q000652:urine; D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D002853:Chromatography, Liquid; D003596:Cytosine / Q000031:analogs & derivatives* / Q000652:urine; D006147:Guanine / Q000031:analogs & derivatives* / Q000652:urine; D006863:Hydrogen-Ion Concentration; D007042:Hypoxanthines / Q000652:urine*; D008099:Liver / Q000032:analysis; D008745:Methylation; D009369:Neoplasms / Q000652:urine; D012343:RNA, Transfer / Q000652:urine*; D051381:Rats
D007042:Hypoxanthines; D006147:Guanine; D003596:Cytosine; D012343:RNA, Transfer; D000225:Adenine