[Concanavalin A- Sepharose affinity chromatography for routine microanalysis of gamma-glutamyltransferase (author's transl)].
The separation of two molecular forms of liver gamma-glutamyltransferase is achieved by Con A-Sepharose chromatography, an adult type with high affinity to Con A and a fetal type without binding capacity to this lectin. Now we present a new method using micro-columns for affinity chromatography (gel volume 2 ml). By this rapid and inexpensive procedure it is possible to study this enzyme separation for its use in the diagnosis of liver diseases.
D002846:Chromatography, Affinity; D003208:Concanavalin A; D006801:Humans; D008107:Liver Diseases / Q000201:enzymology; D008722:Methods; D008832:Microchemistry; D005723:gamma-Glutamyltransferase / Q000302:isolation & purification*
D003208:Concanavalin A; D005723:gamma-Glutamyltransferase
Concanavalin A-Sepharose-Affinitätschromatographie im Mikromassstab fur Serienanalysen am Beispiel der Gamma-Glutamyltransferase.