Characterization of different deoxyribonucleases in human lymphocytes.
Four groups of deoxyribonuclease activities from human lymphocytes have been characterized by deoxyribonuclease assay in DNA-containing polyacrylamide gels following their separation by disc-electrophoresis. All activities hydrolyse DNA endonucleolytically. One neutral deoxyribonuclease found in the cytoplasmic fraction prefers native or UV-irradiated DNA over denatured DNA as substrate and is a 5'-monoester former. Two groups of acid deoxyribonuclease activities are detectable in the nuclear fraction. Both are 3'-monoester formers. One is as well active with denatured DNA as with native DNA, the other one shows the same activity with native and UV-irradiated DNA but lower activity with denatured DNA. An alkaline deoxyribonuclease activity, also localized in the nucleus, is a 5'-monoester former, and prefers denatured or UV-irradiated DNA as substrate.
D002467:Cell Nucleus / Q000201:enzymology; D003593:Cytoplasm / Q000201:enzymology; D004247:DNA / Q000528:radiation effects; D004269:DNA, Bacterial / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism*; D004589:Electrophoresis, Disc; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008214:Lymphocytes / Q000201:enzymology*; D009691:Nucleic Acid Denaturation; D011830:Radiation Effects; D014466:Ultraviolet Rays
D004269:DNA, Bacterial; D004247:DNA; D003851:Deoxyribonucleases