Increase in activity of partially purified alkaline phosphatase after treatment with Mg2+, Zn2+ and lysolecithin.
When lysolecithin predispersed in 0.15 mol/l KCl was sonicated with partially purified alkaline phosphatase, the enzyme activity was increased. Lysolecithin moderately modified the kinetic parameters by increasing the apparent Vmax and decreasing the apparent Km. The pH profile of the enzyme was shifted slightly from a peak at pH 9.5 for the soluble enzyme to a plateau between pH 9.5 and 10.2 for the lysolecithin enzyme mixture. In addition, lysolecithin enhanced the thermostability and resistance of the soluble enzyme preparation to chemical denaturants.
D000469:Alkaline Phosphatase / Q000302:isolation & purification / Q000378:metabolism*; D004789:Enzyme Activation / Q000187:drug effects; D006367:HeLa Cells / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008081:Liposomes; D008244:Lysophosphatidylcholines / Q000494:pharmacology*; D008274:Magnesium / Q000494:pharmacology*; D015032:Zinc / Q000494:pharmacology*
D008081:Liposomes; D008244:Lysophosphatidylcholines; D000469:Alkaline Phosphatase; D008274:Magnesium; D015032:Zinc