Variations in the spectrum of desulfoviridin from Desulfovibrio gigas.
Desulfoviridin preparations from D. gigas showed variations in the position of the absorption maximum the beta-peak) in the 580-nm region of the specturm. On treatment with Na2S2O4 a preparation with a beta-peak at 585 nm was affected rapidly, the 585-nm peak shifting to the 596-nm region; this was partially reversed by K3Fe(CN)6. Treatment of the original preparation with K3Fe(CN)6 resulted in a shift of the beta-peak to 582-583 nm. Desulfoviridins with beta-peaks from 580 to 583 nm were not rapidly affected by Na2S2O4. The spectrum of the chromophore of desulfoviridin way also affected by Na2S2O4 with the peak at 587 nm shifting to 597 nm; this effect was completely reversed by oxygen. There was no evidence to show that spectral variations in desulfoviridin preparations were due to the loss or acquisition of metal ions during growth or to the selection of mutants containing spectrally different desulfoviridins. It is suggested that during biosynethesis oal detachment of the chromophore, thus causing a change towards the spectral properites of the detached chromophore.
D003901:Desulfovibrio / Q000201:enzymology* / Q000254:growth & development; D004227:Dithionite / Q000494:pharmacology; D005292:Ferricyanides / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008670:Metals / Q000494:pharmacology; D010088:Oxidoreductases; D013057:Spectrum Analysis
D005292:Ferricyanides; D008670:Metals; D004227:Dithionite; D010088:Oxidoreductases