Simple assay for glycerophospholipid hydrolase activity of postheparin plasma.
An assay using radioactive substrates is described that permits rapid determinations of glycerophospholipid-hydrolyzing activity in postheparin plasma or its fractions. Optimal conditions are described for hydrolysis of phosphatidylcholine and phosphatidylethanolamine. A minimum of only 2 mul of normal postheparin plasma is used and no extraction of the reaction products is required before their separation by thin-layer chromatography. We found that after an optimal heparin dose of 60 units/kg body weight the rate of hydrolysis for diacyl glycerophosphocholine and diacyl glycerophosphoethanolamine is 1.16 mumoles/ml per hr and 22.4 mumoles/ml per hr, respectively.
D001835:Body Weight; D002265:Carboxylic Ester Hydrolases; D003840:Deoxycholic Acid / Q000494:pharmacology; D004950:Esterases / Q000097:blood*; D006493:Heparin; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008297:Male; D008722:Methods; D010743:Phospholipids; D013696:Temperature
D010743:Phospholipids; D003840:Deoxycholic Acid; D006493:Heparin; D004950:Esterases; D002265:Carboxylic Ester Hydrolases; C023062:glycerophospholipid hydrolase