A sensitive fluorescence assay for the simultaneous and separate determination of arylsulphatases A and B.
A sensitive fluorometric assay utilizing 4-methylumbelliferyl sulphate has been developed for the simultaneous determination of arylsulphatases A and B from leucocytes, based on the differential effect of silver ions on the two enzymes. The procedure allows discrimination between normal cases and those with metachromatic leucodystrophy.
D000293:Adolescent; D002553:Cerebroside-Sulfatase / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D002803:Chondro-4-Sulfatase / Q000097:blood*; D006801:Humans; D006923:Hymecromone; D007962:Leukocytes / Q000201:enzymology; D007966:Leukodystrophy, Metachromatic / Q000201:enzymology; D008722:Methods; D008875:Middle Aged; D012834:Silver; D013050:Spectrometry, Fluorescence; D013429:Sulfatases / Q000097:blood*
D012834:Silver; D006923:Hymecromone; D013429:Sulfatases; D002553:Cerebroside-Sulfatase; D002803:Chondro-4-Sulfatase