Phenylalanine inhibited p-nitrophenyl phosphatase activity in the serum as an indication of intestinal cellular disruption in the horse.
Examination of tissues obtained from thoroughbred horses showed that the 'intestinal' phosphatase activity could be differentiated from other phosphatases by analysis at a pH of 9-5 and inhibition with 15 mM L-phenylalanine. A simple method for the measurement of 'intestinal' phosphatase in heparinised plasma or serum is described. Application of the technique to serum or plasma from normal and diseased horses indicates that the increase in the activity of 'intestinal' phosphatase is associated with cases showing clinical, biochemical and haematological evidence of intestinal damage.
D009597:4-Nitrophenylphosphatase / Q000037:antagonists & inhibitors / Q000097:blood* / Q000302:isolation & purification; D000818:Animals; D005260:Female; D006734:Horse Diseases / Q000201:enzymology*; D006736:Horses / Q000097:blood*; D006863:Hydrogen-Ion Concentration; D007410:Intestinal Diseases / Q000201:enzymology / Q000662:veterinary*; D007422:Intestines / Q000201:enzymology*; D008722:Methods; D010649:Phenylalanine / Q000494:pharmacology*; D010744:Phosphoric Monoester Hydrolases / Q000097:blood*
D010649:Phenylalanine; D010744:Phosphoric Monoester Hydrolases; D009597:4-Nitrophenylphosphatase