Beta-adrenergic receptors in rat myocardium: direct detection by a new fluorescent beta-blocker.
A new fluorescent beta-blocker, 9-amino-acridin propranolol (9-AAP), was administered i.v. to rats. Multiple fluorescent 9-AAP binding sites were observed on cardiac muscle cells in frozen sections. Intensity and density of cardiac 9-AAP fluorescence were markedly reduced following pretreatment with (+/-)- and (-)-propranolol but not with (+)-propranolol. Our findings suggest that 9-AAP may label beta-adrenergic receptor sites in rat myocardium.
D000166:Acridines / Q000032:analysis; D000319:Adrenergic beta-Antagonists; D000818:Animals; D005453:Fluorescence; D008722:Methods; D009206:Myocardium / Q000032:analysis*; D011433:Propranolol / Q000494:pharmacology / Q000031:analogs & derivatives; D051381:Rats; D011941:Receptors, Adrenergic / Q000032:analysis*; D011943:Receptors, Adrenergic, beta / Q000032:analysis*
D000166:Acridines; D000319:Adrenergic beta-Antagonists; D011941:Receptors, Adrenergic; D011943:Receptors, Adrenergic, beta; C013398:9-aminoacridylpropranolol; D011433:Propranolol