Secretion of lecithin: cholesterol acyltransferase from isolated rat hepatocytes.
1. Lecithin:cholesterol acyltransferase is secreted from isolated rat heptocytes. 2. The secretion is stimulated when serum is added to the incubation medium. 3. Optimal conditions for secretion are: 5-10(6) hepatocytes per ml, 5 h incubation, pH 7.3-7.4 and 25% serum in the incubation medium. 4. Concomitantly with the secretion of lecithin:cholesterol acyltransferase there is a secretion of unesterified cholesterol and triacylglycerol. 5. Colchicine or cycloheximide in the incubation medium inhibits secretion of lecithin:cholesterol acyltransferase.
D000217:Acyltransferases / Q000378:metabolism*; D000818:Animals; D001769:Blood; D002784:Cholesterol / Q000378:metabolism; D003078:Colchicine / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D005260:Female; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D007862:Phosphatidylcholine-Sterol O-Acyltransferase / Q000378:metabolism*; D051381:Rats; D014280:Triglycerides / Q000378:metabolism
D014280:Triglycerides; D002784:Cholesterol; D003513:Cycloheximide; D007770:L-Lactate Dehydrogenase; D000217:Acyltransferases; D007862:Phosphatidylcholine-Sterol O-Acyltransferase; D003078:Colchicine