X-ray study of the lithium complex of NAD.
The Li+-NAD+ complex exists as a 'dimer' of two molecules arranged head-to-tail with Li+ coordinated tetrahedrally to adenine N(7) and three pyrophosphate oxygens. Adenine is stacked intermolecularly on nicotinamide. The conformation of NAD+ is 'extended' and similar to that found in holoenzyme complexes. This is in contrast to the 'folded' structure proposed from spectroscopic studies.
D002414:Cations, Monovalent; D003461:Crystallography; D011756:Diphosphates; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008094:Lithium; D008958:Models, Molecular; D008968:Molecular Conformation; D009243:NAD / Q000378:metabolism; D009249:NADP; D011485:Protein Binding; D014961:X-Ray Diffraction
D002414:Cations, Monovalent; D011756:Diphosphates; D009243:NAD; D009249:NADP; D008094:Lithium; D007770:L-Lactate Dehydrogenase