Stabilization of human serum alkaline phosphatase to histidine-induced heat inactivation by tryptic digestion.
1. Serum alkaline phosphatase [EC 3.1.3.1] was strongly inactivated by histidine during incubation at pH 8.0 and 45degrees; however, tryptic digestion of the serum strongly protected the enzyme against inactivation by histidine. In the absence of histidine, however, neither heat inactivation of the phosphatase nor the effect of trypsin [EC 3.4.21.4] was observed. Factors affecting the alkaline phosphatase inactivation were studied further. 2. The effect of trypsin on the histidine-induced heat inactivation differed considerably according to the tissue source of the enzyme, which suggests a possible method for distinguishing alkaline phosphatase isoenzymes.
D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors / Q000097:blood*; D001842:Bone and Bones / Q000201:enzymology; D004492:Edetic Acid / Q000494:pharmacology; D005260:Female; D006639:Histidine / Q000494:pharmacology*; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007422:Intestines / Q000201:enzymology; D007527:Isoenzymes / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D008274:Magnesium / Q000494:pharmacology; D010920:Placenta / Q000201:enzymology; D011247:Pregnancy; D014357:Trypsin
D007527:Isoenzymes; D006639:Histidine; D004492:Edetic Acid; D000469:Alkaline Phosphatase; D014357:Trypsin; D008274:Magnesium