Oxisuran reduction by rabbit tissue preparations.
Oxisuran, 2-((methylsulfinyl)acetyl)pyridine is reduced to alpha-((methylsulfinyl)methyl-2-pyridinemethanol, oxisuranol, by cytoplasmic enzymes from rabbit liver, kidney, brain, intestine, and lung. The cytoplasmic enzyme from liver is dependent on NADPH as cofactor and has an optimal pH of 6.0. Enzymatic activity is also present in liver mitochondria but at a lower specific activity. The cytoplasmic extracts catalyze the formation of two oxisuranol products, presumably the diastereoisomers described by Di Carlo and associates from in vivo studies. Verification of the product as oxisuranol was accomplished by thin-layer chromatography and mass spectrometry.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007421:Intestine, Small / Q000378:metabolism*; D007668:Kidney / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008168:Lung / Q000378:metabolism*; D013058:Mass Spectrometry; D008930:Mitochondria, Liver / Q000378:metabolism; D009249:NADP; D009928:Organ Specificity; D010084:Oxidation-Reduction; D011725:Pyridines / Q000378:metabolism*; D011817:Rabbits
D011725:Pyridines; D009249:NADP