[Luminescence study of the effect of temperature on the conformational state of fibrinogen].
Results are presented of measuring fibrinogen fluorescence parameters in temperature range of 20-80 degrees C at different pH of the solution. It was found that the temperature increase from 20 to 40 degrees C for solutions with pH of 4,5-9,3 were not accompanied by the conformational changes of fibrinogen macromolecules. In the temperature range of 40-50 degrees C for neutral solutions conformational reconstruction of fibrinogen of undenatured character took place. Temperature increase above 50-55 degrees C brings about significant structural changes of fibrinogen molecule which are of denaturation nature.
D005340:Fibrinogen; D006863:Hydrogen-Ion Concentration; D008968:Molecular Conformation; D013050:Spectrometry, Fluorescence; D013696:Temperature
D005340:Fibrinogen
Liuminestsentnoe issledovanie vliianiia temperatury na konformatsionnoe sostoianie fibrinogena