G6PD Vientiane: a new glucose-6-phosphate dehydrogenase variant with increased stability.
A new G6PD variant, called G6PD Vientiane, has been discovered in a patient from Laos. The characteristics of this variant are: mild enzyme deficiency (about 50% of the normal activity) in the granulocytes and the red cells, with normal G6PD-related antigen concentration; increased stability; normal Km glucose 6-phosphate and NADP+; increased inhibition constant by NADPH; decreased inhibition by ATP; slightly increased utilization of the substrate analogue; abnormal pH curve, with maximum activity at pH 9.5; slightly reduced starch gel electrophoretic migration. The implications of the molecular stability of a deficient mutant variant are discussed.
D000328:Adult; D004592:Electrophoresis, Starch Gel; D005602:France; D014644:Genetic Variation; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007812:Laos / Q000208:ethnology; D008297:Male; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism
D009243:NAD; D009249:NADP; D005954:Glucosephosphate Dehydrogenase