Estimation of testosterone binding capacity in the serum with hydrophobie resin.
A method for the estimation of the serum testosterone binding capacity (TeBC) using hydrophobic resin, Amberlite XAD-2, was developed. The serum was incubated with a saturating amount of testosterone-1,2(-3)H at 15 degrees C, unbound testosterone was adsorbed to the resin and the 3H-radoiactivity remaining in the supernatant fluid was counted. Under these conditions, the normal levels and their standard deviations were 15.08+/-3.39 ng/ml (n=7) for male and 35.06+/-3.56 ng/ml (n=6) for female respectively. Precision of the method was 6.29%. TeBC in the third month of pregnancy was approximately 1.5 times more than that of non-pregnant women, and approximately 3 to 5 times in the tenth month.
D000418:Albumins; D002352:Carrier Proteins / Q000097:blood; D004958:Estradiol; D005260:Female; D006801:Humans; D006854:Hydrocortisone; D006863:Hydrogen-Ion Concentration; D008297:Male; D011247:Pregnancy; D012116:Resins, Plant; D013696:Temperature; D013739:Testosterone / Q000097:blood*
D000418:Albumins; D002352:Carrier Proteins; D012116:Resins, Plant; D013739:Testosterone; D004958:Estradiol; D006854:Hydrocortisone