[Solubilization and purification of a 3alpha-hydroxysteroid dehydrogenase in rat liver microsomes (author's transl)].
The microsomal 3-hydroxysteroid dehydrogenases were solubilized with lubrol, a non-ionic detergent. A 3alpha-hydroxysteroid dehydrogenase is purified about 100-fold by double affinity chromatography on 5alpha-dihydrotestosterone-Sepharose. This enzyme can use both NADH and NADPH as coenzymes.
D000818:Animals; D006913:Hydroxysteroid Dehydrogenases / Q000302:isolation & purification* / Q000378:metabolism; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D009243:NAD; D009249:NADP; D051381:Rats; D012995:Solubility; D013501:Surface-Active Agents
D013501:Surface-Active Agents; D009243:NAD; D009249:NADP; D006913:Hydroxysteroid Dehydrogenases
Solubilisierung und Anreicherung einer 3alpha-Hydroxysteroid-Dehydrogenase aus Rattenleber-Mikrosomen