Effects of phospholine iodide on the metabolites of the glycolytic, pentose phosphate and sorbitol pathways in the rabbit lens.
Steady-state concentrations of the key intermediates from the glycolytic, pentose phosphate, and sorbitol pathways as well as the pyridine nucleotides were measured from the lens after 0.25% phospholine iodide had been instilled into rabbits' eyes twice a day for 18 weeks. In the lenses of those rabbits which had received treatment in both eyes fructose-1,6-diphosphate and pyruvate levels were increased, whereas 6-phosphogluconate, sorbitol and alpha-glycer0phosphate concentrations were decreased. alpha-Ketoglutarate and concentrations and ratios of NAD+ and NADH did not show any changes. In contrast, NADPH and total NADP concentrations as well as the NADPH/NADP+ ratio were decreased, and therefore total NAD/total NADP ratio increased after treatment. It appears that instillation of long-acting 0.25% phospholine iodide into rabbits' eyes results in increased glycolytic activity in the lens in response to the increased energy demand, wheras the activities of other metabolic pathways are suppressed.
D000818:Animals; D004456:Echothiophate Iodide / Q000494:pharmacology*; D005338:Fibrin Fibrinogen Degradation Products / Q000378:metabolism; D005636:Fructosephosphates / Q000378:metabolism; D005942:Gluconates / Q000378:metabolism; D005994:Glycerophosphates / Q000378:metabolism; D006019:Glycolysis / Q000187:drug effects*; D007656:Ketoglutaric Acids / Q000378:metabolism; D007908:Lens, Crystalline / Q000201:enzymology / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010428:Pentosephosphates / Q000378:metabolism*; D011725:Pyridines / Q000378:metabolism; D011773:Pyruvates / Q000378:metabolism; D011817:Rabbits; D013012:Sorbitol / Q000378:metabolism*
D005338:Fibrin Fibrinogen Degradation Products; D005636:Fructosephosphates; D005942:Gluconates; D005994:Glycerophosphates; D007656:Ketoglutaric Acids; D010428:Pentosephosphates; D011725:Pyridines; D011773:Pyruvates; D009243:NAD; D013012:Sorbitol; D009249:NADP; D004456:Echothiophate Iodide