Inhibition by alanine of AMP-deaminase from rabbit skeletal muscle.
1. Alanine inhibits rabbit muscle AMP-deaminase while aspartate, histidine and glutamate are ineffective. 2. The degree and type of inhibition of AMP-deaminase by alanine depend on pH; at pH 6.5 alanine behaves like an allosteric effector exerting a negative heterotropic effect. At pH 7.0 the inhibition is non-competitive, Ki being as high as 19 mM. 3. The probable significance of the effect of alanine on AMP-deaminase in muscle metabolism is discussed.
D000659:AMP Deaminase / Q000037:antagonists & inhibitors*; D000409:Alanine / Q000494:pharmacology*; D000494:Allosteric Regulation; D000818:Animals; D001224:Aspartic Acid / Q000494:pharmacology; D005971:Glutamates / Q000494:pharmacology; D006639:Histidine / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009132:Muscles / Q000201:enzymology*; D009709:Nucleotide Deaminases / Q000037:antagonists & inhibitors*; D011817:Rabbits; D013329:Structure-Activity Relationship
D005971:Glutamates; D001224:Aspartic Acid; D006639:Histidine; D009709:Nucleotide Deaminases; D000659:AMP Deaminase; D000409:Alanine