Identification of dimethylnitrosoamine metabolites in vitro.
The incubation of dimethylnitrosoamine (DMNA) in the presence of rat liver microsomes leads to production of formaldehyde, formic acid, methylamine, and N-methylhydrazine. When pH 5-enzymes are added to the medium there is also the formation of N-methylhydroxylamide and N,N-dimethylhydrazine. The last compound is the only metabolite produced, to a lesser extent, by the pH 5-enzymes. Thus, the denitrosated or non-denitrosated metabolites are produced either by an oxidative dealkylation and by a reduction of DMNA, catalysed by microsomal and cellular soluble enzymes.
D000818:Animals; D004128:Dimethylnitrosamine / Q000378:metabolism*; D004798:Enzymes / Q000378:metabolism; D005260:Female; D005561:Formates / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008099:Liver / Q000201:enzymology; D008744:Methylamines / Q000378:metabolism; D008862:Microsomes, Liver / Q000378:metabolism; D009002:Monomethylhydrazine / Q000378:metabolism; D009602:Nitrosamines / Q000378:metabolism*; D051381:Rats
D004798:Enzymes; D005561:Formates; D008744:Methylamines; D009602:Nitrosamines; D005978:Glutathione; D004128:Dimethylnitrosamine; D009002:Monomethylhydrazine