[New values for the molar extinction coefficients of NADH and NADPH for the use in routine laboratories (author's transl)].
Extensive re-investigations with regard to the molar extinction coefficients of NADH and NADPH proved that in future, calculations in routine work can be performed with the following much more accurate epsilon-values: 6.15 x 10(3) 1 x mol-1 x cm-1 at Hg 334 nm (NADH and NADPH), 6.3 X 10(3) 1 X mol-1 x cm-1 at 340 nm (NADH and NADPH), 3.4 X 10(3) 1 X mol-1 X Cm-1 (NADH) and 3.5 x 10(3) 1 x mol-1 x cm-1 (NADPH) at Hg 365 nm, respectively. The safest measurement is performed at Hg 334 nm, because here epsilon is identical for both coenzymes and deviations of the epsilon-value caused by temperature, pH and ionic strength are less than 0.5%.
D003124:Colorimetry; D006863:Hydrogen-Ion Concentration; D009243:NAD / Q000592:standards*; D009249:NADP / Q000592:standards*; D011786:Quality Control; D013696:Temperature
D009243:NAD; D009249:NADP
Neue Werte für die molaren Extinktions-Koeffizienten von NADH und NADPH  zum Gebrauch im Routine-Laboratorium