The dynamics of production and the sensitivity to cycloheximide and actinomycin D of interferon-inducing and interferon messenger RNA.
In the process of virus-induced interferon production, two kinds of RNA appear in the cells. One of them induces production by the recipient cells of interferon with the species-specificity of the latter cells (interferon-inducing RNA), and the other is translated by the recipient cells pre-treated with actinomycin D into interferon with the species-specificity of the donor cells of RNA (interferon mRNA). The interferon-inducing RNA appears 20-30 minutes after virus induction and shows maximal activity after 1 hour. Its formation is not influenced by cycloheximide or actinomycin D. This RNA is assumed to be a transcriptive intermediate form of viral RNA. Interferon mRNA appears in the cells 1 hour after virus induction and shows maximal activity after 6-8 hours. Its synthesis is inhibited by cycloheximide and actinomycin D.
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D002642:Chick Embryo; D046508:Culture Techniques; D003513:Cycloheximide / Q000494:pharmacology*; D003593:Cytoplasm / Q000378:metabolism; D003609:Dactinomycin / Q000494:pharmacology*; D007372:Interferons / Q000096:biosynthesis*; D007739:L Cells; D009522:Newcastle disease virus / Q000378:metabolism; D012313:RNA / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis*; D012367:RNA, Viral / Q000096:biosynthesis*; D013045:Species Specificity
D012333:RNA, Messenger; D012367:RNA, Viral; D003609:Dactinomycin; D012313:RNA; D007372:Interferons; D003513:Cycloheximide