Cultivation of Lednice (Yaba 1) virus in goose, duck, and chick embryo cells.
Lednice (Yaba 1) virus was found to multiply in primary cultures of goose, duck and chick embryo cells. The size of inoculum and age of embryos used for preparation of primary cultures was decisive for the amount of multiplied virus. One strain (110) of Lednice virus showed reduced ability to induce a cytopathic effect in the first passage in chick embryo cells. Virus multiplication in all three kinds of cells was confirmed by immunofluorescence.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002642:Chick Embryo; D046508:Culture Techniques; D003588:Cytopathogenic Effect, Viral; D004372:Ducks; D005455:Fluorescent Antibody Technique; D005777:Geese; D014776:Virus Cultivation; D014779:Virus Replication; D014999:Yaba monkey tumor virus / Q000254:growth & development* / Q000276:immunology
D000956:Antigens, Viral