Influence of inorganic phosphate and organic buffers on cephalosporin production by Streptomyces clavuligerus.
A high concentration of potassium phosphate (75--100 mM) stabilized pH and supported extensive growth of Streptomyces clavuligerus in a chemically defined medium; such a concentration also inhibited cephalosporin production. Although Tris buffer was found to have detrimental effects on growth and antibiotic production, 3-(N-morpholine)-propane sulfonate (MOPS) or 2-(NP-morpholine)-ethane sulfonate (MES) buffer provided a nontoxic buffering system. In the presence of MOPS buffer, cephalosporin production was optimal at 25 mM phosphate, whereas higher concentrations of phosphate progressively inhibited antibiotic production up to 85% without modifying the pH pattern. MOPS buffer can be used to conduct fermentations at a relatively constant pH value in shake flasks.
D000476:Alkanesulfonates / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000096:biosynthesis; D002021:Buffers; D002511:Cephalosporins / Q000096:biosynthesis*; D002513:Cephamycins / Q000096:biosynthesis; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D007769:Lactams / Q000096:biosynthesis; D009025:Morpholines / Q000494:pharmacology*; D010406:Penicillins / Q000096:biosynthesis; D010710:Phosphates / Q000494:pharmacology*; D013302:Streptomyces / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D014325:Tromethamine / Q000494:pharmacology
D000476:Alkanesulfonates; D000900:Anti-Bacterial Agents; D002021:Buffers; D002511:Cephalosporins; D002513:Cephamycins; D007769:Lactams; D009025:Morpholines; D010406:Penicillins; D010710:Phosphates; D014325:Tromethamine; C008550:morpholinopropane sulfonic acid