The interaction of reduced nicotinamide--adenine dinucleotide phosphate with reduced nicotinamide--adenine dinucleotide--ubiquinone reductase from bovine heart mitochondria.
Reduction of the chromophores of mitochondrial NADH-ubiquinone reductase by NADPH reaches only 50% of the extent of reduction by NADH, monitored at 450 nm. This effect is due to autoxidation of an enzyme component at a higher rate than its reduction by NADPH.
D000818:Animals; D002417:Cattle; D005292:Ferricyanides; D006884:Hydroxybutyrate Dehydrogenase / Q000378:metabolism; D006885:Hydroxybutyrates / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000494:pharmacology; D008931:Mitochondria, Muscle / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism; D009243:NAD / Q000378:metabolism*; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D009249:NADP / Q000378:metabolism*
D005292:Ferricyanides; D006885:Hydroxybutyrates; D009243:NAD; D009249:NADP; D007770:L-Lactate Dehydrogenase; D006884:Hydroxybutyrate Dehydrogenase; D009247:NADH, NADPH Oxidoreductases