[Role of adrenoreceptors in regulating aspartate aminotransferase isoenzyme activity in albino rat hearts].
Cytoplasmic (c) and mitochondrial (m) isoenzymes of aspartate aminotransferase (AAT, EC 2.6.1.1.) were isolated from rat heart extracts by electrophoresis in agar gel. Their pH optima and Km values were estimated; optimal conditions for estimation of the enzymatic activities are reported. Isadrine activated and adrenaline inhibited the cAAT activity. Noradrenaline did not affect the activity of both isoenzymes. Phentolamine, as contrary to obsidane which decreased the activity of both isoenzyme, activated the isoenzymes; the effect was partially decreased by obsidane. Phentolamine did not alter the noradrenaline effect on either mAAT or cAAT; it decreased significantly the free form of the mAAT activity only. Results of the experiments with administration of adrenomimetic drugs suggested that adrenaline and noradrenaline-isadrine had different sites of attachment through which they mediated their action on aspartate aminotransferase in rat heart mitochondria.
D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism*; D003593:Cytoplasm / Q000201:enzymology; D004837:Epinephrine / Q000494:pharmacology; D006321:Heart / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007527:Isoenzymes / Q000378:metabolism*; D007545:Isoproterenol / Q000494:pharmacology; D008297:Male; D008929:Mitochondria, Heart / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology*; D009638:Norepinephrine / Q000494:pharmacology; D010646:Phentolamine / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology; D051381:Rats; D011941:Receptors, Adrenergic / Q000502:physiology*; D013565:Sympatholytics / Q000494:pharmacology
D007527:Isoenzymes; D011941:Receptors, Adrenergic; D013565:Sympatholytics; D011433:Propranolol; D001219:Aspartate Aminotransferases; D007545:Isoproterenol; D009638:Norepinephrine; D004837:Epinephrine; D010646:Phentolamine
Rol' adrenoretseptorov v reguliatsii aktivnosti izofermentov aspartat-aminotransferazy serdtsa belykh krys.