[Incorporation in vivo of 14C-precursors into pyrimidine nucleotides of chicken liver and spleen tissues].
Maximum incorporation of the label after administration of 14C-bicarbonate to free pyrimidine nucleotides begins by the 60th minute. Radioactivity of cytidine nucleotides at all time points is four-eight times as low as that of uridine nucleotides. 2-14C-orotic acid is incorporated into acid-soluble nucleotides and RNA of the liver and spleen tissues. 60 min after administration of the isotope 20% of radioactivity was found in the liver and 0.3% in the spleen, in both tissues radioactivity of RNA is 20% of 14C-radioactivity in the acid-soluble fraction. The formation rate for cytidine nucleotides from uridine ones in the liver and spleen tissues is low, about 2-6% of uridine nucleotides radioactivity is found in them only, that may be due to a low activity of CTP-synthetase. The content of cytidine nucleotides in these organs is three-life times as low as compared to uridine nucleotides.
D000818:Animals; D001639:Bicarbonates / Q000378:metabolism; D002645:Chickens; D003597:Cytosine Nucleotides / Q000096:biosynthesis; D006863:Hydrogen-Ion Concentration; D008025:Ligases / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D009963:Orotic Acid / Q000378:metabolism; D011742:Pyrimidine Nucleotides / Q000096:biosynthesis*; D012313:RNA / Q000096:biosynthesis; D012995:Solubility; D013154:Spleen / Q000378:metabolism*; D014500:Uracil Nucleotides / Q000378:metabolism
D001639:Bicarbonates; D003597:Cytosine Nucleotides; D011742:Pyrimidine Nucleotides; D014500:Uracil Nucleotides; D009963:Orotic Acid; D012313:RNA; D008025:Ligases
Vkliuchennia in vivo 14C-poperednikiv u pirimidinovi nukleotidi tkanin pechinki i selezinki kurchat.