[Study of phenothiazine binding to membranes by the fluorescent probe method].
Binding of chlorpromazine, etaperazine and triftazine with model phospholipid membranes was investigated by using a 3-methoxybenzanthrone (MBA) fluorescent probe. All these preparations interacted with the membranes, this being registered by the MBA fluorescence extinction. The phenothiazine molecules bound with the membrane are localized in its superficial layer and extinguish the MBA fluorescence in different ways. The chlorpromazine binding was heterogenous and there are, apparently, 2 different ways of its molecules combination with the membranes. The effective extinction of MBA by phenothiazine increased in the following order: chlorpromazine, etaperazine, triftazine.
D014150:Antipsychotic Agents / Q000378:metabolism*; D001551:Benz(a)Anthracenes; D001665:Binding Sites; D002746:Chlorpromazine / Q000378:metabolism; D008081:Liposomes; D008563:Membrane Lipids / Q000378:metabolism*; D010546:Perphenazine / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D014268:Trifluoperazine / Q000378:metabolism
D014150:Antipsychotic Agents; D001551:Benz(a)Anthracenes; D008081:Liposomes; D008563:Membrane Lipids; D014268:Trifluoperazine; C015345:methoxybenzanthrone; D010546:Perphenazine; D002746:Chlorpromazine
Izuchenie sviazyvaniia fenotiazinov s membranami metodom fliuorestsentnykh zondov.