In situ localization of galactosyltransferase in surface mucous cells of the rat stomach.
Cryostate sections of the rat stomach fundus were incubated in the presence of uridine 5'-diphosphate-[3H]-galactose. The radioactivity in the surface mucous epithelial cells was shown by autoradiography to be specifically incorporated into a supranuclear area, the area where in these cells the Glogi system is situated. The incorporation lasted only 5-6 min and was Mn++ dependent. Galactose was probably incorporated into a beta-glycosidic bond.
D000818:Animals; D001345:Autoradiography; D005690:Galactose / Q000378:metabolism; D005700:Galactosyltransferases / Q000032:analysis*; D005753:Gastric Mucosa / Q000201:enzymology* / Q000378:metabolism; D006056:Golgi Apparatus / Q000201:enzymology; D006863:Hydrogen-Ion Concentration; D008297:Male; D008345:Manganese / Q000494:pharmacology; D051381:Rats
D008345:Manganese; D005700:Galactosyltransferases; D005690:Galactose