Fluoresceinylthiocarbamyl-tRNATyr: a useful derivative of tRNATyr (E.coli) for physicochemical studies.
Fluoresceinylthiocarbamyl-tRNATyr (FTC-tRNATyr) is prepared from tRNATyr and fluoresceinisothiogyanate (FITC) under mildly alkaline conditions. Labelling occurs specificly at the base Q of tRNATyr. The modified tRNA is fully active in the aminoacylation assay; when aminoacylated it is recognized by the elongation factor Tu (EF-Tu). Codon-anticodon interaction, however, is severely affected by the modification.
D000926:Anticodon; D003062:Codon; D004926:Escherichia coli / Q000378:metabolism*; D005452:Fluoresceins; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008722:Methods; D010445:Peptide Elongation Factors; D012343:RNA, Transfer / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry; D013859:Thiocarbamates; D014443:Tyrosine
D000926:Anticodon; D003062:Codon; D005452:Fluoresceins; D010445:Peptide Elongation Factors; D013859:Thiocarbamates; D014443:Tyrosine; D012343:RNA, Transfer