[Chemiluminescence of the blood serum in the presence of divalent iron salts].
On incubation of diluted blood serum with ferrous ions kinetics of chemiluminescence, typical for lipid containing systems, was observed. It comprised several sequental steps: quick flash, inhibition of the chemiluminescence, slow flash with subsequent steady-state luminescence. At each step of the phenomenon a correlation was found between the intensity of chemiluminescence, the rate of accumulation of products of lipid peroxidation (malone dialdehyde) and concentration of Fe2+ in the mixture. The data obtained suggest that chemiluminescence of blood serum in presence of Fe2+ is due to lipid peroxidation in the serum. Kinetics of the reaction and shape of the chemiluminiscence curve depended upon a number of factors: dilution of blood serum, concentration of Fe2+ added, temperature, pH and (if blood plasma was used) type of an anticoagulant use. Only in blood lipoproteins, mainly in beta-lipoproteins, the distinctive pattern of peroxidation was developed with simultaneous accumulation of malone dialdehyde, oxidation of ferrous ions and ultralow chemiluminescence. In protein fraction of blood serum addition of Fe2+ caused only the quick splash of chemiluminescence without the malone dialdehyde production.
D000818:Animals; D001769:Blood / Q000187:drug effects* / Q000378:metabolism; D001798:Blood Proteins / Q000378:metabolism; D002413:Cations, Divalent; D004285:Dogs; D005296:Ferrous Compounds / Q000494:pharmacology; D005609:Free Radicals; D006863:Hydrogen-Ion Concentration; D050356:Lipid Metabolism; D008163:Luminescent Measurements; D008315:Malondialdehyde / Q000378:metabolism; D010084:Oxidation-Reduction; D010545:Peroxides / Q000378:metabolism; D013696:Temperature
D001798:Blood Proteins; D002413:Cations, Divalent; D005296:Ferrous Compounds; D005609:Free Radicals; D010545:Peroxides; D008315:Malondialdehyde
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