[Isolation of rat liver chromatin non-histone protein].
A chromatin with the protein/DNA ratio of 3.0 was obtained from rat liver cells nuclei. The chromatin was dissolved in 2 M NaCl, pH 7, and reprecipitated by decreasing the ionic strength to 0.4 and increasing pH to 9.0. A fraction of non-histone proteins (NH-1) remained in a supernatant solution, the NH-1/DNA ratio being equal to 1.3 and the ratio of acidic to basic aminoacid residues equal to 1.31. After chromatography on Bio-Rex 70 cation-exchang resin, a fraction (NH-2) with the NH-2/DNA ratio of 1.0 +/- 0.15 and the ratio of acidic to basic aminoacid residues of 1.57 was obtained. According to the data of polyacrylamide gel electrophoresis, the NH-2 fraction contained 2.7% of histone f2 + f3 as an admixture whereas histone f1 was not revealed.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002843:Chromatin / Q000032:analysis*; D003852:Deoxyribonucleoproteins / Q000302:isolation & purification; D006657:Histones; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000032:analysis*; D009698:Nucleoproteins / Q000302:isolation & purification*; D051381:Rats
D000596:Amino Acids; D002843:Chromatin; D003852:Deoxyribonucleoproteins; D006657:Histones; D009698:Nucleoproteins
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