Studies on the inhibition of the desaturases by cyclopropenoid fatty acids.
Unwashed rat liver microsomes were used to study the inhibition of the delta6 and delta9 desaturases by cyclopropenoid fatty acids with the ring structure about the 9,10 or 6,7 carbon atoms. The 9,10 cyclopropenoid acid (sterculic acid) is shown to be an effective inhibitor of only delta9 desaturase and then only in the presence of MgCl2 and coenzyme A (presumably due to the formation of sterculoyl-CoA). Two 6,7 cyclopropenoid acids of different chain lengths showed no marked inhibition of either the delta6 or delta9 desaturase. By the use of [3H]-sterculic acid, it has been shown that under conditions of high inhibition of the delta9 desaturase the inhibitor is not covalently attached to the enzyme at any point. This disproves older ideas on the mechanism of inhibition that assumed reaction between the cyclopropenoid ring and sulphydryl groups on the enzymes.
D000818:Animals; D003065:Coenzyme A / Q000494:pharmacology; D003521:Cyclopropanes; D044943:Fatty Acid Desaturases / Q000037:antagonists & inhibitors*; D005229:Fatty Acids, Monounsaturated; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D008274:Magnesium / Q000494:pharmacology; D008862:Microsomes, Liver / Q000187:drug effects* / Q000201:enzymology; D009243:NAD / Q000494:pharmacology; D009249:NADP / Q000494:pharmacology; D051381:Rats
D003521:Cyclopropanes; D005229:Fatty Acids, Monounsaturated; D005231:Fatty Acids, Unsaturated; D009243:NAD; D009249:NADP; D044943:Fatty Acid Desaturases; D008274:Magnesium; C003345:sterculic acid; D003065:Coenzyme A