A Dacron wool packed-bed extracorporeal reactor: a kinetic study of immobilized Escherichia coli II L-asparaginase.
An extracorporeal reactor containing a packed bed of Dacron fibers has been developed. Escherichia coli II L-asparaginase was coupled to the Dacron using gamma-aminopropyltriethoxysilane and glutaraldehyde. The preparation had an activity of 37 IU per gram of Dacron (37 degrees C). The apparent Km was studied as a function of the flow rate. The data indicated that the apparent Km approached the Km of the native enzyme at flow rates of about 300 mg/min. In vivo use of L-asparaginase immobilized on the Dacron indicated effective lowering of plasmatic L-asparagine levels.
D000818:Animals; D001215:Asparaginase / Q000378:metabolism*; D004285:Dogs; D004926:Escherichia coli / Q000201:enzymology; D006355:Heart-Lung Machine / Q000295:instrumentation*; D006863:Hydrogen-Ion Concentration; D011093:Polyethylene Terephthalates; D013696:Temperature; D013784:Textiles; D014935:Wool
D011093:Polyethylene Terephthalates; D001215:Asparaginase