[Effect of cortisol on the content of mRNA coding inducible tyrosine aminotransferase isoenzyme in rat liver].
In vitro estimation of synthesis of inducible tyrosine aminotransferase isoenzyme, directed by poly-A-containing RNA from liver of intact and corticol treated rats, is carried out. Total poly-A-containing RNA from liver polyribosomes of intact and induced rats was translated in cell-free system from wheat germs. Two antibodies immunoprecipitation was used to identify the translocation product (tyrosine aminotransferase). It was found that a synthesis of a specific protein product, precipitated by antibodies to tyrosine aminotransferase, takes place in cell-free system under translation of polysomic poly-A-containing liver RNA. The amount of immunoprecipitated product indicates, that the content of individual poly-A-containing mRNA for inducible tyrosine aminotransferase isoenzyme in liver of cortisol-induced rats is considerably higher than in intact animals.
D000818:Animals; D004790:Enzyme Induction / Q000187:drug effects; D006854:Hydrocortisone / Q000494:pharmacology*; D007527:Isoenzymes / Q000096:biosynthesis*; D008099:Liver / Q000378:metabolism*; D011132:Polyribosomes / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D014444:Tyrosine Transaminase / Q000096:biosynthesis*
D007527:Isoenzymes; D012333:RNA, Messenger; D014444:Tyrosine Transaminase; D006854:Hydrocortisone
Vliianie kortizola na soderzhanie mRNK, kodiruiushcheĭ indutsibel'nyi izoferment tirozinaminotransferazy v pecheni krys.