A thin-layer chromatographic procedure for the assay of labelled noradrenaline and its metabolites in tissues and in incubation medium.
A method for the extraction, separation and quantitative determination of [3H]noradrenaline [3H-NA] and its five major metabolites has been devised using thin layer chromatography. This procedure was used to study the pattern of 3H-NA and its metabolites in the total radioactivity of the tissues and that released spontaneously from the rat isolated vas deferens. Whereas in tissues 3H-NA represented almost all of the total radioactivity (94-8+/-0.47%), in the samples of spontaneous outflow it represented only 16-8+/-2.1%. The rest was mostly accounted for by the five metabolites, primarily 3H-DOPEG and 3H-MOPEG. These findings show that in the rat vas deferens 3H-NA is preferentially metabolized via the two glycol derivatives, i.e. 3H-DOPEG and 3H-MOPEG.
D000818:Animals; D002855:Chromatography, Thin Layer / Q000379:methods*; D008297:Male; D009638:Norepinephrine / Q000032:analysis* / Q000378:metabolism; D051381:Rats; D014316:Tritium; D014649:Vas Deferens / Q000032:analysis / Q000378:metabolism
D014316:Tritium; D009638:Norepinephrine