Reactions of cellulose isothiocyanates with thiol and amino compounds.
A cellulose isothiocyanate has been prepared by treatment of cellulose with 2,4-di-isocyanatotoluene followed by hydrolysis and reaction of the resulting amine with thiophosgene. The cellulose isothiocyanate was characterized by its binding capacity with respect to [14C]-glycine, [131 I]-human serum albumin, and 2-mercaptoethanol. An analytical method for binding capacity, based on reaction with [35 S]-alpha-toluenethiol, was developed. Because of the aromatic character of the NCS group of the cellulose isothiocyanate, the covalently bonded thiol can be quantitatively liberated.
D001665:Binding Sites; D002482:Cellulose; D005998:Glycine; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008623:Mercaptoethanol; D011485:Protein Binding; D012709:Serum Albumin; D013861:Thiocyanates
D012709:Serum Albumin; D013861:Thiocyanates; D008623:Mercaptoethanol; D002482:Cellulose; D005998:Glycine