Poly(8-aminoguanylic acid): formation of ordered self-structures and interaction with poly(cytidylic acid).
Poly(8-aminoguanylic acid) has in neutral solution a novel ordered structure of high stability. The 8-amino group permits formation of three hydrogen bonds between two residues along the "top", or long axis, of the purines. The usual hydrogen bonding protons and Watson-Crick pairing sites are not involved in the association. The bonding scheme has a twofold rotation axis and is hemiprotonated at N(7). Poly(8NH2G) is converted by alkaline titration (pK = 9.7) to a quite different ordered structure, which is the favored form over the range approximately pH 10-11. The bonding scheme appears to be composed of a planar, tetrameric array of guanine residues, in which the 8-amino group does not participate in interbase hydrogen bonding. Poly (8NH2G) does not interact with poly(C) in neutral solution because of the high stability of the hemiprotonated G-G self-structure. Titration to the alkaline plateau, however, permits ready formation of a two-stranded Watson-Crick helix. In contrast to the monomer 8NH2GMP, poly(8NH2G) does not form a triple helix with poly(C) under any conditions. The properties of the ordered structures are interpreted in terms of a strong tendency of the 8-amino group to form a third interbase hydrogen bond, when this possibility is not prevented by high pH.
D001665:Binding Sites; D002942:Circular Dichroism; D004355:Drug Stability; D004926:Escherichia coli / Q000201:enzymology; D006860:Hydrogen Bonding; D006863:Hydrogen-Ion Concentration; D008837:Micrococcus / Q000201:enzymology; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D011066:Poly C; D011068:Poly G / Q000031:analogs & derivatives*; D011117:Polyribonucleotide Nucleotidyltransferase / Q000378:metabolism; D011131:Polyribonucleotides / Q000031:analogs & derivatives*; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature
D011131:Polyribonucleotides; D011068:Poly G; D011066:Poly C; D011117:Polyribonucleotide Nucleotidyltransferase