Dc Polarographic assay of tetracyclines.
The inhibitory effect of dioctyl sodium sulfosuccinate on hog pepsin activity was investigated over the pH 1.5-3.0 range. The inhibitory effect was studied using a natural substrate, hemoglobin, and a synthetic substrate, N-acetyl-L-diiodotyrosine. The mechanistic studies revealed that a substrate-inhibitor interaction was the major mechanism of inhibition with hemoglobin. However, some direct enzyme inhibition also was involved. With the synthetic substrate, the inhibition las due to a competition between the substrate and the inhibitor molecules for the enzyme. The possible therapeutic significance of the inhibitory effect of the medicinal surfactant is discussed.
D002021:Buffers; D004304:Dosage Forms; D006863:Hydrogen-Ion Concentration; D011048:Polarography / Q000379:methods; D013754:Tetracyclines / Q000032:analysis*
D002021:Buffers; D004304:Dosage Forms; D013754:Tetracyclines