Hemoglobin function in stored blood. XIII. A citrate-adenine preservative with optimal pH to maintain red cell 2,3-DPG (function) and ATP (viability).
Increasing pH by a 0.5 increment over the commonly used preservative, acid-citrate-dextrose with adenine (ACD-Ad), results in a significant improvement in 2,3-DPG, with no significant loss in concentrations of ATP. The intermediate pH preservative, 6.0, also had ATP concentrations which equaled those of the low pH preservatives, 5.0 and 5.5, from the 21st to the 42nd day of storage. A citrate-adenine preservative, with a pH between 5.5 and 6.0, would seem to be optimal for maintenance of hemoglobin function and red cell viability, as determined by measurements of 2,3-DPG and ATP concentrations.
D000255:Adenosine Triphosphate / Q000378:metabolism*; D001793:Blood Preservation; D002951:Citrates / Q000494:pharmacology*; D004163:Diphosphoglyceric Acids / Q000378:metabolism*; D004905:Erythrocyte Aging / Q000187:drug effects*; D006454:Hemoglobins / Q000502:physiology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D013997:Time Factors
D002951:Citrates; D004163:Diphosphoglyceric Acids; D006454:Hemoglobins; D000255:Adenosine Triphosphate