Quantitative analysis of tribromsalan in blood and urine.
Tribromsalan can be quantitatively measured in whole blood and urine by a technique involving extraction with ethyl acetate, treatment with silica gel, separation by TLC, and quantitative measurement by fluorescent spectrophotometry. This method has a sensitivity down to 125 ng (25 ppb in 5.0 ml of sample) of free tribromsalan and shows an average 90% recovery of tribromsalan in blood and urine with standard deviations of 9.7 and 7.4%, respectively.
D000818:Animals; D002855:Chromatography, Thin Layer; D004202:Disinfectants / Q000032:analysis* / Q000097:blood / Q000652:urine; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008722:Methods; D011817:Rabbits; D012457:Salicylamides / Q000032:analysis* / Q000097:blood / Q000652:urine; D012458:Salicylanilides; D013050:Spectrometry, Fluorescence
D004202:Disinfectants; D012457:Salicylamides; D012458:Salicylanilides; C004361:tribromsalan