[Intracellular nucleodepolymerase of bacteria, representatives of the Proteus and Providencia groups. Its isolation and properties].
High activity of enzymes, splitting native, denaturated DNA, deoxyribooligonucleotides and RNA, was observed in cell free extracts of bacteria--representatives of 5 strains of Proteus-Providencia. Some properties of nucleases were studied in cell free extracts. In the bacteria studied DNAases were thermolabile proteins, which were completely inactivated at 50-60 degrees, RNAases were more thermostable. The pH optima of the DNAases were at pH 9.0-11.0 in cell free system; RNAases were maximally active at pH 8.0-9.0
D002458:Cell Fractionation; D002474:Cell-Free System; D003851:Deoxyribonucleases / Q000378:metabolism*; D004355:Drug Stability; D004789:Enzyme Activation; D004926:Escherichia coli / Q000201:enzymology; D006863:Hydrogen-Ion Concentration; D011489:Protein Denaturation; D011511:Proteus / Q000201:enzymology*; D011513:Proteus mirabilis / Q000201:enzymology; D011514:Proteus vulgaris / Q000201:enzymology; D011532:Providencia / Q000201:enzymology*; D012260:Ribonucleases / Q000378:metabolism*; D013696:Temperature
D003851:Deoxyribonucleases; D012260:Ribonucleases
Vnutrikletochnye nukleodepolimerazy bakteriĭ--predstaviteleĭ gruppy Proteus i Providencia. Poluchenie i nekotorye svoĭstva