Proton-induced uptake of mammalian DNA by dog erythrocyte pink ghosts.
Low pH (below 6) induces the uptake of mammalian DNA in dog erythrocyte pink ghosts. Uptake requires either Ca2+ or Mg2+ and is stimulated by ATP. These agents induce a rapid sphering of the ghosts at 37 degrees C and sphering is required for uptake. Uptake is increased in ghosts which have been incubated 60-90 min before adding the DNA. Uptake is strongly temperature-dependent. Lowering the temperature of a suspension of ghosts taking up DNA at 37-0 degrees C stops uptake. It is concluded that uptake depends on active membrane processes and that it may depend on the capacity of the ghosts to maintain cation exchange.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D004247:DNA / Q000378:metabolism*; D003851:Deoxyribonucleases / Q000494:pharmacology; D004285:Dogs; D004910:Erythrocyte Membrane / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008214:Lymphocytes; D008274:Magnesium / Q000494:pharmacology*; D013696:Temperature
D000255:Adenosine Triphosphate; D004247:DNA; D003851:Deoxyribonucleases; D008274:Magnesium; D002118:Calcium