Characterization of the mannosyl and fucosyl transferases in the ovine anterior pituitary glands.
Ovine anterior pituitary glands contain mannosyl- and fucosyl-transferases localized in the microsomes and able to incorporate mannose or fucose as such from GDP-mannose or GDP-fucose into endogenous glycoproteins. The requirements and conditions necessary for maximum activity were investigated. The value of the Km is very similar for the two enzyme systems, 3 X 10(-7) M in the case of mannosyl-transferases and 5 X 10(-7) M in the case of fucosyl-transferases.
D000818:Animals; D006154:Guanosine Diphosphate Fucose / Q000378:metabolism*; D006155:Guanosine Diphosphate Mannose / Q000378:metabolism; D006602:Hexosyltransferases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D008364:Mannosyltransferases / Q000378:metabolism*; D008861:Microsomes / Q000201:enzymology; D009702:Nucleoside Diphosphate Sugars / Q000378:metabolism*; D010902:Pituitary Gland / Q000201:enzymology*; D010903:Pituitary Gland, Anterior / Q000201:enzymology*; D012756:Sheep; D013696:Temperature
D009702:Nucleoside Diphosphate Sugars; D006154:Guanosine Diphosphate Fucose; D006155:Guanosine Diphosphate Mannose; D008345:Manganese; D006602:Hexosyltransferases; D008364:Mannosyltransferases; D008274:Magnesium