A gel filament as an analytical tool: the testing of an affinity chromatographic, product-immobilizing linear gel for determination of enzymatic activity.
A new technique for quantitative analysis is proposed. An underfined volume of the test solution is exposed to a reagent in the form of a gel filament of capillary dimensions. The linear progress in the gel of the compound in question is followed with the aid of the coloured reaction products deposited along its path. With the use of the enzyme gamma-glutamyl transpeptidase it is shown that the velocity in the system of the component to be analysed, as determined by measurement of its coloured track, is related to its activity in the test solution.
D000814:Aniline Compounds; D002846:Chromatography, Affinity / Q000379:methods*; D003116:Color; D007700:Kinetics; D009574:Nitro Compounds; D005723:gamma-Glutamyltransferase / Q000032:analysis*
D000814:Aniline Compounds; D009574:Nitro Compounds; D005723:gamma-Glutamyltransferase