[Biosynthesis of fatty acids in mouse brain mitochondria in the presence of malonyl-CoA or acetyl-CoA].
Incorporation of malonyl-CoA or acetyl-CoA is studied in mouse brain mitochondrial fatty acids. Rupture of mitochondria is necessary ; Triton X-100 gives the best result. Other detergents or sonication are of lesser efficiency. Cofactor requirements have been studied : NADH and NADPH have been tested ; ATP increases biosynthesis and CoA causes an inhibition. Two systems of biosynthesis are involved : -- One is a de novo system using malonyl-CoA. Malonyl-CoA alone is incorporated and synthesizes mainly C16, indicating the existence of a malonly-CoA decarboxylase although elongation of short chain fatty acids cannot be excluded. Addition of acetyl-CoA increases the biosynthesis and palmityl-CoA when added causes an inhibition. -- The other system, using acetyl-CoA, elongates exogenous palmityl-CoA ; endogenous acyl-CoAs are not elongated by acetyl-CoA. All these results are confirmed by radiogas chromatographic studies of the reactions products.
D000105:Acetyl Coenzyme A / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002458:Cell Fractionation; D003065:Coenzyme A / Q000378:metabolism* / Q000494:pharmacology; D003902:Detergents / Q000494:pharmacology; D005227:Fatty Acids / Q000096:biosynthesis* / Q000302:isolation & purification; D066298:In Vitro Techniques; D008314:Malonates / Q000378:metabolism; D051379:Mice; D008928:Mitochondria / Q000378:metabolism*; D009243:NAD / Q000494:pharmacology; D009249:NADP / Q000494:pharmacology; D010169:Palmitic Acids / Q000378:metabolism; D011092:Polyethylene Glycols / Q000494:pharmacology
D003902:Detergents; D005227:Fatty Acids; D008314:Malonates; D010169:Palmitic Acids; D009243:NAD; D011092:Polyethylene Glycols; D009249:NADP; D000105:Acetyl Coenzyme A; D000255:Adenosine Triphosphate; D003065:Coenzyme A
Biosynthèse des acides gras dans les mitochondries de cerveau de souris en résence de malonyl-CoA ou d'acétyl-CoA