Cell wall autolysis in log phase cells of Micrococcus lysodeikticus (luteus).
Log phase cells of Micrococcus lysodeikticus (luteus) IFO 3333 autolyzed when incubated at 37 C in 0.01 M sodium-phosphate buffer pH 7.5. The enzyme involved in the autolysis was recovered mainly in an aqueous phase from cytoplasmic membranes and cytoplasmic materials treated with n-butanol, and proved to be an N-acetylmuramyl-L-alanine amidase. The autolysis of log phase cells suspended in autolyzing buffer was depressed by the addition of trypsin to the buffer.
D000362:Agar / Q000494:pharmacology; D000409:Alanine; D000581:Amidohydrolases / Q000032:analysis; D001329:Autolysis / Q000378:metabolism*; D002021:Buffers; D002473:Cell Wall / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D003470:Culture Media; D003593:Cytoplasm / Q000187:drug effects; D003851:Deoxyribonucleases / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008837:Micrococcus / Q000254:growth & development / Q000378:metabolism*; D009112:Muramic Acids; D013395:Sucrose; D014357:Trypsin / Q000494:pharmacology
D002021:Buffers; D003470:Culture Media; D009112:Muramic Acids; D013395:Sucrose; D000362:Agar; D003851:Deoxyribonucleases; D014357:Trypsin; D000581:Amidohydrolases; D000409:Alanine