Determination of the Tamm and Horsfall glycoprotein in human urine.
A method for the routine determination of the Tamm and Horsfall glycoprotein in human urine is presented. The method is based on quantitative electroimmunoassay. It was demonstrated that the solubility of the Tamm and Horsfall glycoprotein was dependent on urinary ionic strength and pH. To minimize these variables all urines were buffered to pH 5.5 and then preincubated at 37 degrees C in 0.3% sodium dodecyl sulphate. This procedure solubilized and maintained the Tamm and Horsfall glycoprotein in solution. Excess sodium dodecyl sulphate was removed by gel filtration before the quantities of the Tamm and Horsfall glycoprotein were determined by electroimmunoassay. The method is simple, reproducible, has high precision and compares favourably with earlier published methods for the determinations of the Tamm and Horsfall glycoprotein.
D000596:Amino Acids / Q000032:analysis; D006023:Glycoproteins / Q000302:isolation & purification / Q000652:urine*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D008970:Molecular Weight; D009994:Osmolar Concentration; D012967:Sodium Dodecyl Sulfate; D013696:Temperature
D000596:Amino Acids; D006023:Glycoproteins; D012967:Sodium Dodecyl Sulfate