[Hydroxylation of testosterone and 5alpha-dihydrotestosterone--isolation of 2alpha-hydroxytestosterone (author's transl)].
The hydroxylation at C-2 of testosterone and 5alpha-dihydrotestosterone is measured by isolation of HTO after incubating "metabolically labile" tritium labeled steroids with the enzymes. After hydroxylation of testosterone in rat liver 2alpha-hydroxytestosterone was isolated and identified by gaschromatography and mass spectra.
D000818:Animals; D003534:Cyproterone / Q000494:pharmacology; D013196:Dihydrotestosterone / Q000378:metabolism*; D004958:Estradiol / Q000378:metabolism; D006900:Hydroxylation; D006915:Hydroxytestosterones / Q000378:metabolism*; D008297:Male; D013058:Mass Spectrometry; D008797:Metyrapone / Q000494:pharmacology; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D009249:NADP / Q000378:metabolism; D051381:Rats; D013739:Testosterone / Q000031:analogs & derivatives* / Q000378:metabolism*
D006915:Hydroxytestosterones; D013196:Dihydrotestosterone; D013739:Testosterone; D004958:Estradiol; D009249:NADP; D003534:Cyproterone; D008797:Metyrapone
Hydroxylierung von Testosteron und 5alpha-Dihydrotestosteron in Rattenleber. Isolierung von 2alpha-Hydroxytestosteron