Placenta-like alkaline phosphatases from human osteosarcoma cells.
Hormone-induced alkaline phosphatases in human osteosarcoma cells (LM) were extracted and purified. Characterization of the purified enzyme showed two distinct isoenzymes. One isoenzyme was heat labile, was homoarginine inhibited, and had the electrophoretic migration of alkaline phosphatase of human osseous origin. Immunodiffusion showed that this isoenzyme reacted positively only against anti-bone alkaline phosphatase antibodies. The second isoenzyme was heat stable, was inhibited by phenylalanie, and had the same electrophoretic migration as did alkaline phosphatase extracted from mature normal human placenta. This second isoenzyme had the same antigenicity as did the normal placental enzyme. Like the D-variant placental phenotype, this second isoenzyme was inhibited by L-leucine and ethylenediaminetetraacetic acid.
D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000378:metabolism*; D001842:Bone and Bones / Q000201:enzymology; D002478:Cells, Cultured; D004790:Enzyme Induction / Q000187:drug effects; D005260:Female; D006358:Hot Temperature; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000378:metabolism*; D009374:Neoplasms, Experimental / Q000201:enzymology; D012516:Osteosarcoma / Q000201:enzymology*; D010649:Phenylalanine / Q000494:pharmacology; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy
D007527:Isoenzymes; D010649:Phenylalanine; D000469:Alkaline Phosphatase; D006854:Hydrocortisone