Presence of a third sucrose hydrolyzing enzyme in Bacillus subtilis: constitutive levanase synthesis by mutants of Bacillus subtilis Marburg 168.
A beta-D-fructofuranosidase -- called levanase -- capable of the hydrolysis of sucrose, inulin and levans has been identified in Bacillus subtilis Marburg. This enzyme can not be detected in strain 168. However, sacL mutations -- mapped on the chromosome of strain 168 between the pheA and aroD reference markers -- lead to constitutive levanase synthesis. This synthesis is repressed by carbon sources such as glucose, glycerol or sucrose.
D001412:Bacillus subtilis / Q000201:enzymology*; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D004794:Enzyme Repression; D005838:Genotype; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000096:biosynthesis*; D007700:Kinetics; D009154:Mutation; D011995:Recombination, Genetic; D013045:Species Specificity; D013393:Sucrase / Q000096:biosynthesis* / Q000378:metabolism; D014161:Transduction, Genetic
D007527:Isoenzymes; D013393:Sucrase