Stabilization of human fibroblast interferon purified on concanavalin A-agarose.
Human fibroblast interferon, obtained by chromatography on concanavalin A-agarose, was stable for at least a month in 30--50 per cent ethylene glycol at 4 degrees, --20 degrees, and --70 degrees C. The succinct point of the present finding is that human fibroblast interferon may be stabilized by ethylene glycol alone without the addition of bovine serum albumin and 'back-contamination' of the interferon preparation.
D002846:Chromatography, Affinity; D003080:Cold Temperature; D003208:Concanavalin A; D004121:Dimethyl Sulfoxide; D005026:Ethylene Glycols; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007372:Interferons / Q000302:isolation & purification; D011309:Preservation, Biological / Q000379:methods*; D012685:Sepharose; D013757:Tetraethylammonium Compounds
D005026:Ethylene Glycols; D013757:Tetraethylammonium Compounds; D003208:Concanavalin A; D007372:Interferons; D012685:Sepharose; D004121:Dimethyl Sulfoxide