The effect of antibody against a purified sperm-coating antigen on human sperm.
A purified fraction of human seminal plasma containing a sperm-coating antigen and two minor contaminants was used to immunize rabbits by intravenous route. An antiserum containing only the antibody against the sperm-coating antigen as examined by immunoelectrophoresis was obtained from one rabbit. The effect of this antiserum on human sperm was examined by sperm-immobilization and sperm-agglutination tests. The results revealed that the antibody against the purified sperm-coating antigen was incapable of immobilzing or agglutinating human sperm. This indicates that the purified sperm-coating antigen is unlikely to be useful as an antifertility antigen for immunologic fertility control.
D000372:Agglutination Tests; D000818:Animals; D000906:Antibodies; D000941:Antigens / Q000302:isolation & purification; D006801:Humans; D007106:Immune Sera / Q000008:administration & dosage; D007114:Immunization; D007122:Immunoelectrophoresis; D007275:Injections, Intravenous; D008297:Male; D011817:Rabbits; D013081:Sperm Motility; D013094:Spermatozoa / Q000276:immunology*
D000906:Antibodies; D000941:Antigens; D007106:Immune Sera