The sensitivity of chromatin from thymuses and spleens of irradiated mice to alkaline solutions.
Increasing amounts of DNA and proteins are released from the suspensions of chromatin from thymuses and spleens of irradiated mice (6 hours after 600R whole-body) by the action of alkaline solutions (pH 8-10) at physiological ionic strengths. The suspension of chromatin from normal tissues releases in this pH range only a small amount of proteins and negligible amount of DNA. The behaviour of liver and kidney chromatin to alkaline solutions shows no difference between normal and irradiated tissues. The time of onset and dose relation of the increased sensitivity of thymus and spleen chromatin from irradiated mice to alkaline solutions show a similar course as earlier described signs of postirradiation damage to chromatin of these tissues.
D000468:Alkalies; D000818:Animals; D002843:Chromatin / Q000032:analysis / Q000528:radiation effects*; D004247:DNA / Q000032:analysis; D005260:Female; D006863:Hydrogen-Ion Concentration; D051379:Mice; D012996:Solutions; D013154:Spleen / Q000528:radiation effects*; D013950:Thymus Gland / Q000528:radiation effects*
D000468:Alkalies; D002843:Chromatin; D012996:Solutions; D004247:DNA