[The coupling of beta1-24-corticotropin to the adenylate-cylase system in rat adipocytes. Evidence for hormone-nucleotides interaction (author's transl)].
The general aim was to define some of the most important parameters involved in the coupling step between the synthetic analog of adrenocoricotropin hormone  (beta1-24-corticotropin tetracosa peptide) and the catalytic unit of the adenylate-cyclase system of fat cells.  These studies were performed with a purified plasma membrane fraction from rat adipose tissue.  In this regard, some effects of ions, pH, and nucleotides (ATP nad GTP) on this hormone sensitive system were studied A simple model based on a random association process of reactants yeilded a statisfactory approximation of the kinetic data.  In contract to results obtained by two other groups, which were analyzed by De Haen, no evidence was found for a regulation of the adenylate-cyclase activity by the adenosine triphosphate which was not complexed to magnesium...
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000262:Adenylyl Cyclases / Q000378:metabolism; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000324:Adrenocorticotropic Hormone / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008297:Male; D008433:Mathematics; D009994:Osmolar Concentration; D011485:Protein Binding; D051381:Rats
D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D000324:Adrenocorticotropic Hormone; D000262:Adenylyl Cyclases; D002118:Calcium
Couplage de la corticotropine-beta1-24 avec le système adénylate cyclase des adipocytes de rat. Mise en évidence d'une interaction hormone-nucléotides