Formation and dissimilation of oxalacetate and pyruvate Pseudomonas citronellolis grown on noncarbohydrate substrates.
Metabolism of lactate as a carbon source by Pseudomonas citronellolis occurred via a nicotinamide adenine dinucleotide (NAD)-independent L-lactate dehydrogenase, which was present in cells grown on DL-lactate but was not present in cells grown on acetate, aspartate, citrate, glucose, glutamate, or malate. The cells also possessed a constitutive, NAD-independent malate dehydrogenase instead of the conventional NAD-dependent malate dehydrogenase instead of the conventional NAD-dependent enzyme in the tricarboxylic acid cycle. Both enzymes were particulate and used dichlorophenolindo-phenol or oxygen as an electron acceptor. In acetate-grown cells, the activity of pyruvate dehydrogenase and NAD phosphate-linked malate enzyme decreased, cells grown on glucose or lactate. This was consistent with the need to maintain a supply of oxalacetate for metabolism of acetate via the tricarboxylic acid cycle. Changes in enzyme activities suggest that gluconeogenesis from noncarbohydrate carbon sources occurs via the malate enzyme (when oxalacetate decarboxylase is inhibited) or a combination of the NAD-independent malate dehydrogenase and oxalacetate decarboxylase.
D000085:Acetates / Q000378:metabolism; D002952:Citric Acid Cycle; D004790:Enzyme Induction; D007770:L-Lactate Dehydrogenase / Q000096:biosynthesis; D007773:Lactates / Q000378:metabolism; D008291:Malate Dehydrogenase / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010071:Oxaloacetates / Q000378:metabolism*; D011549:Pseudomonas / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D011773:Pyruvates / Q000378:metabolism*
D000085:Acetates; D007773:Lactates; D010071:Oxaloacetates; D011773:Pyruvates; D009249:NADP; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase