On RNA-polymerases of leukemia L 1210 origin and an enzymatic method to screen antitumor antibiotics.
Four DNA-dependent RNA-polymerases were separated from the cell homogenate of moust leukemia L1210 cell by DEAE-cellulose column chromatography and tentatively designated as Peaks I, II, III and IV in the elution order. Peak II was inactivated by the addition of alpha-amanitin and effects of antibiotics and enzymes on the RNA-polymerase activity using Peaks, I, II and a mixture of Peaks I and II were examined. The RNA-polymerases were used to screen for enzyme inhibitors produced by microbes. This enzymatic method was successfully proved to select antitumor antibiotics.
D000546:Amanitins / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D002848:Chromatography, DEAE-Cellulose; D012321:DNA-Directed RNA Polymerases / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism; D004353:Drug Evaluation, Preclinical; D006863:Hydrogen-Ion Concentration; D006867:Hydrolases / Q000494:pharmacology; D007939:Leukemia L1210 / Q000201:enzymology*; D051379:Mice
D000546:Amanitins; D000900:Anti-Bacterial Agents; D000903:Antibiotics, Antineoplastic; D012321:DNA-Directed RNA Polymerases; D006867:Hydrolases