Erythrocyte organic phosphates in the anemia of renal failure in childhood.
Erythrocyte 2,3-diphosphoglycerate (2,3-DPG ) and adenosinetriphosphate (ATP) levels were determined in 43 children with chronic renal failure on conservative treatment (CT), and 12 children on regular hemodialysis (HD) immediately before and after a HD session. The results were compared to non-anemic and anemic controls. In spite of anemia, erythrocyte 2,3-DPG in renal failure was similar to non-anemic controls at normal blood pH, but rose during dialysis as a result of alkalosis. In contrast, ATP levels were high already at a normal blood pH. 2,3-DPG correlated with packed cell volume (PCV) in children with renal failure but at lower concentrations compared to controls. Both organic phosphates in the erythrocytes showed a significant correlation with blood pH. The poor increase of 2,3-DPG, in combination with elevated ATP levels, suggests uremia-induced inhibition of 2,3-DPG synthesis.
D000255:Adenosine Triphosphate / Q000097:blood*; D000293:Adolescent; D000471:Alkalosis / Q000209:etiology; D000740:Anemia / Q000097:blood* / Q000209:etiology; D002648:Child; D004163:Diphosphoglyceric Acids / Q000097:blood*; D004912:Erythrocytes / Q000032:analysis*; D006400:Hematocrit; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007676:Kidney Failure, Chronic / Q000097:blood* / Q000150:complications; D006435:Renal Dialysis
D004163:Diphosphoglyceric Acids; D000255:Adenosine Triphosphate