[Examination of the action of effectors after storing glutamine synthetase from the fodder yeast Candida tropicalis at room temperature].
The pattern of action of effectors (AMP, IMP, CMP, anthranilic acid, tryptophane, alanine, glutamine, glycine, histidine) and glutamine synthetase (GS) extracted from the fodder yeast Candida tropicalis and stored at room temperature for 1.5-2 hrs was examined. As regards the action of effectors on GS after its exposure at room temperature, they can be subdivided into four groups: 1) the effector loses completely its inhibitory effect (glutamine, CMP, anthranilic acid in the synthetase reaction); 2) the inhibitory effect on the enzyme increases (AMP, IMP, anthranilic acid in the transferase reaction); 3) at low concentrations of the effector the peak of activation appears (tryptophane, GMP, alanine, glycine); 4) at low concentrations of the enzyme two peaks of activation appear (histidine). Similar results were obtained with the purified preparation of GS.
D000596:Amino Acids / Q000494:pharmacology*; D002175:Candida / Q000201:enzymology*; D004355:Drug Stability; D004789:Enzyme Activation; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism*; D007700:Kinetics; D012265:Ribonucleotides / Q000494:pharmacology*; D013696:Temperature
D000596:Amino Acids; D012265:Ribonucleotides; D005974:Glutamate-Ammonia Ligase
Issledovanie kharaktera deistviia effektorov posle vyderzhivaniia gliutaminsintetazy kormovykh drozhzheĭ pri komnatnoĭ temperature.