Electrodecantation of serum proteins.
The sedimentation of albumin under the action of the electric and gravitational fields was determined as a function of time in discontinuous experiments in a rectangular cell, using serum with the albumin fraction stained blue. It was shown that even under the influence of strong electric fields, the upper boundary of the albumin layer fell no further than the mid-point of the cell. In continuous single-stage separation of gamma-globulin from other serum proteins, only about half the gamma-globulins can be obtained from the solution because it remains homogeneously distributed throughout the solution and is only free from albumin and other proteins in the upper half of the cell. In experiments with continuously operated triangular cells, the process was optimized to give gamma-globulin of 97.5% purity in a yield of 80%, at serum flow-through rates of up to 0.5 l/h in a block composed of 40 cells.
D000818:Animals; D001797:Blood Protein Electrophoresis / Q000379:methods; D001798:Blood Proteins / Q000302:isolation & purification*; D004553:Electric Conductivity; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008433:Mathematics; D012709:Serum Albumin / Q000302:isolation & purification; D012712:Serum Globulins / Q000302:isolation & purification
D001798:Blood Proteins; D012709:Serum Albumin; D012712:Serum Globulins