Interactions of bradykinin, prostaglandin E1, 5-hydroxytryptamine, histamine and adenosine-5'-triphosphate on the dry leakage response in rat skin.
Dye leakage in rats produced by intracutaneous injections of irritants was quantitated using an azovan blue technique. Concentration-response lines were obtained for irritants alone and in presence of constant concentrations of other irritants. 5-Hydroxytryptamine (5-HT) (10-7M) potentiated histamine, bradykinin and prostaglandin E1 (PGE1) but inhibited adenosine-5'-triphosphate (ATP). Histamine (10-5M) potentiated bradykinin, PGE1 and ATP. Bradykinin (10-6M) potentiated PGE1 only, but PGE1 (10-6M) potentiated bradykinin, 5-HT and histamine. ATP (10-4M) potentiated 5-HT, histamine and PGE1. Release of ATP during stimulation of sensory nerves might explain enhanced neurogenic oedema observed in skin surrounding an area pretreated with compound 48/80.
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D001920:Bradykinin / Q000494:pharmacology*; D004396:Coloring Agents / Q000378:metabolism*; D004357:Drug Synergism; D006632:Histamine / Q000494:pharmacology*; D008297:Male; D011458:Prostaglandins E / Q000494:pharmacology*; D051381:Rats; D012701:Serotonin / Q000494:pharmacology*; D012867:Skin / Q000378:metabolism*
D004396:Coloring Agents; D011458:Prostaglandins E; D012701:Serotonin; D006632:Histamine; D000255:Adenosine Triphosphate; D001920:Bradykinin