Association-dissociation of the flavoprotein hog kidney D-amino acid oxidase. Determination of the monomer-dimer equilibrium constant and the energetics of subunit association.
The enzyme concentration dependence of spectrophotometric titrations of hog kidney D-amino acid oxidase [EC 1.4.3.3] with p-aminobenzoate was studied. The monomer-dimer equilibrium constant of the oxidized holoenzyme at 25 degrees C was estimated to be 7 X 10(5)M-1 at pH 7.5 and 4X 10(6)M-1 at pH 8.3. The energetics of subunit association are discussed.
D010129:4-Aminobenzoic Acid; D000818:Animals; D003605:D-Amino-Acid Oxidase; D005420:Flavoproteins; D007668:Kidney / Q000201:enzymology*; D007700:Kinetics; D046911:Macromolecular Substances; D008433:Mathematics; D013053:Spectrophotometry; D013552:Swine; D013816:Thermodynamics
D005420:Flavoproteins; D046911:Macromolecular Substances; D003605:D-Amino-Acid Oxidase; D010129:4-Aminobenzoic Acid