Glycogen synthesis by amylosucrase from Neisseria perflava.
Amylosucrase (sucrose:1,4-alpha-D-glucan 4-alpha-glucosyltransferase; EC 2.4.1.4) which mediates the transfer of the glucosyl moiety of sucrose to a growing alpha-1,4-glucan chain is a constitutive enzyme of Neisseria perflava. The products of enzymic action are insoluble glycogenlike polysaccharides and fructose, the latter being a competitive inhibitor of the enzyme (Ki=20 mM). The enzyme is extremely stable and appears to bind very tightly to its polymerized product. Properties of product-bound enzyme reflect those of the native complex.
D002474:Cell-Free System; D004355:Drug Stability; D005632:Fructose / Q000096:biosynthesis*; D005964:Glucosyltransferases / Q000378:metabolism*; D006003:Glycogen / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D009343:Neisseria / Q000201:enzymology* / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism
D005632:Fructose; D013395:Sucrose; D006003:Glycogen; D005964:Glucosyltransferases