Production of extracellular alpha-glucosidase by a thermophilic Bacillus species.
Production of extracellular alpha-glucosidase was studied with strain KP 1006 of a new species of thermophilic Bacillus, which was isolated from soil samples by enrichment at 65 C. alpha-Glucosidase production was maximum at 60 C and at an initial pH of 6.5. The final enzyme yield was increased by starch, maltose, glycerol, peptone, and yeast extract but reduced by acetate and gluconate, alpha-Glucosidase was formed in the cytoplasm and accumulated as a large pool during the logarithmic growth phase. At a midpoint of this period, the enzyme appeared in the culture broth, and its level increased until the end of the stationary phase.
D001407:Bacillus / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D050260:Carbohydrate Metabolism; D005285:Fermentation; D005959:Glucosidases / Q000096:biosynthesis* / Q000378:metabolism; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008320:Maltose / Q000378:metabolism; D013213:Starch / Q000378:metabolism
D008320:Maltose; D013213:Starch; D005959:Glucosidases