Cross-linking of nucleosomal histones with monofunctional imidoesters.
Cross-linking experiments with the MONOfunctional imidoester methyl-acetimidate, in the pH range 7.0 - 8.0, on rat liver nucleosomes generate a cross-linking pattern almost identical with the one observed for much longer BIfunctional reagents (e.g. dimethylsuberimidate). Combined cross-linking and trypsin digestion experiments suggest that all or at least the great majority of this cross-linking occurs on trypsin digestible segments (or "tails") of the histones. The formation of oligomers over such extremely short distances and especially the observation of an H3 homodimer suggests a very close proximity of half-nucleosomes.
D000818:Animals; D002843:Chromatin / Q000648:ultrastructure*; D004120:Dimethyl Suberimidate; D006657:Histones; D006863:Hydrogen-Ion Concentration; D007094:Imides; D008099:Liver / Q000648:ultrastructure; D008970:Molecular Weight; D011485:Protein Binding; D051381:Rats
D002843:Chromatin; D006657:Histones; D007094:Imides; D004120:Dimethyl Suberimidate