[Phospholipase of rat intestine: mode of action].
Phospholipase activities of rat intestinal mucosa homogenate have been determined from lysophosphatidylcholines [14C] and phosphatidylcholines [-3H-14C]. In the presence of phosphatidylcholines, at pH 6.5, the homogenate has a phospholipase B activity. At  pH 8.5, a phospholipase A2 activity was shown. In the presence of lysophospatidylcholines, at pH 6.5, we notice a lysophospholipase A1 activity. A kinetic study of the reactions allows us to separate the activity B into a phospholipase A2 activity and a lysophospholipase A1 activity. Thus, it appears that the total phospholipase activity of rat intestinal mucosa would results from a phospholipase A2 activity and a lysophospholipase A1 activity.
D000818:Animals; D002250:Carbon Radioisotopes; D006863:Hydrogen-Ion Concentration; D007413:Intestinal Mucosa / Q000201:enzymology*; D007421:Intestine, Small / Q000201:enzymology*; D007553:Isotope Labeling; D007700:Kinetics; D008244:Lysophosphatidylcholines; D010713:Phosphatidylcholines; D010740:Phospholipases / Q000378:metabolism*; D051381:Rats; D013329:Structure-Activity Relationship; D014316:Tritium
D002250:Carbon Radioisotopes; D008244:Lysophosphatidylcholines; D010713:Phosphatidylcholines; D014316:Tritium; D010740:Phospholipases
Phospholipases de l'intestin de rat: mode d'action.