[Photosensitization of consitutents of nucleic acids by proflavine. Mechanism of formation of hydrogen addition radicals in frozen aquenous solutions (author's transl)].
It is shown that the insertion of nucleotides between proflavine molecules is favourable to photosensitization. Furthermore (1) each molecule of proflavine gives at the most one free radical in the substrate, (2) the chromophore is largely restored when oxygen is not present, (3) superoxide radicals are observed in the presence of oxygen, and (4) formyl radicals are detected. The scheme elaborated for the mechanism gives an explanation for all these observations.
D000166:Acridines; D001665:Binding Sites; D003854:Deoxyribonucleotides; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals; D005615:Freezing; D006863:Hydrogen-Ion Concentration; D008968:Molecular Conformation; D009690:Nucleic Acid Conformation; D010777:Photochemistry; D011370:Proflavine; D014867:Water
D000166:Acridines; D003854:Deoxyribonucleotides; D005609:Free Radicals; D014867:Water; D011370:Proflavine
Photosensibilisation des constituants des acides nucléiques par la proflavine Mécanisme de formation de radicaux d'addition d'hydrogène en solutions aqueuses gelées