Soluble enzyme system for vitamin K-dependent carboxylation.
The vitamin K-dependent carboxylating system has been solubilized by Lubrol PX or Triton X-100 treatment of vitamin K-deficient rat liver microsomes. As obtained from vitamin K-deficient rat liver, this soluble preparation is dependent upon the in vitro addition of vitamin K1 for carboxylating activity. The enzyme system is complex and is dependent upon NADH and dithiothreitol for maximum activity. While detergents used to solubilize the enzyme complex do markedly inhibit the activity of the system, the solubilized system is still highly responsive to vitamin K addition and can be used for further study of the carboxylating enzyme system. The requirement for dithiothreitol and the inhibition by p-hydroxymercuribenzoate indicate the involvement of an --SH enzyme in the carboxylating system.
D000818:Animals; D002262:Carboxy-Lyases / Q000302:isolation & purification / Q000378:metabolism*; D003600:Cytosol / Q000187:drug effects / Q000201:enzymology; D003902:Detergents; D004229:Dithiothreitol / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D006902:Hydroxymercuribenzoates / Q000494:pharmacology; D005779:Immunodiffusion; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology*; D009243:NAD / Q000494:pharmacology; D051381:Rats; D012995:Solubility; D014812:Vitamin K / Q000494:pharmacology*; D014813:Vitamin K Deficiency / Q000201:enzymology*
D003902:Detergents; D006902:Hydroxymercuribenzoates; D009243:NAD; D014812:Vitamin K; D002262:Carboxy-Lyases; D004229:Dithiothreitol