Evidence that fatty acid synthesis in the interscapular brown adipose tissue of cold-adapted rats is increased in vivo by insulin by mechanisms involving parallel activation of pyruvate dehydrogenase and acetyl-coenzyme A carboxylase.
Plasma insulin concentrations in cold-adapted rats were altered acutely by administration of glucose or anti-insulin serum. Rates of fatty acid synthesis in interscapular brown adipose tissue were determined from the incorporation of 3H from 3H2O into tissue lipid. Rates of synthesis were greatly elevated after glucose administration and markedly decreased after injection with anti-insulin serum. Parallel changes in the initial activities of both acetyl-CoA carboxylase and pyruvate dehydrogenase were observed under these conditions, but no changes in total activities were evident. The results suggest that this tissue is an important site of fatty acid synthesis in the cold-adapted rat and that this feature of the tissue is sensitive to changes in plasma insulin concentrations.
D000103:Acetyl-CoA Carboxylase / Q000378:metabolism*; D000222:Adaptation, Physiological; D002001:Adipose Tissue, Brown / Q000201:enzymology / Q000378:metabolism*; D000818:Animals; D003080:Cold Temperature; D005227:Fatty Acids / Q000096:biosynthesis*; D005260:Female; D005947:Glucose / Q000378:metabolism; D007328:Insulin / Q000378:metabolism; D008025:Ligases / Q000378:metabolism*; D011768:Pyruvate Dehydrogenase Complex / Q000378:metabolism*; D011773:Pyruvates / Q000378:metabolism; D051381:Rats
D005227:Fatty Acids; D007328:Insulin; D011768:Pyruvate Dehydrogenase Complex; D011773:Pyruvates; D008025:Ligases; D000103:Acetyl-CoA Carboxylase; D005947:Glucose