[Anion-sensitive nuclear ATPase of the rat heart].
Properties of HCO--3-stimulated ATPase from rat heart muscle nuclei were studied. The maximal activity of HCO--3-ATPase was observed at concentration of bicarbonate 25 mM. The enzyme had a pH optimum at pH 8.0-8.5. Bicarbonate stimulated the ATPase activity only in presence of Mg2+, Mn2+ and Zn2+, Co2+, Cd2+ and Ca2+ were ineffective. NaCO3 and Na2SO3 at concentration 30 mM stimulated the nuclear ATPase activity by 20% and 81%, respectively. Anions N3--, scn--, clO--4, and I-- inhibited both Mg2+-ATPase and HCO--3-ATPase. HSO--3 and SO2--4 ions did not affect the nuclear ATPase activity.
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D000838:Anions; D001639:Bicarbonates / Q000494:pharmacology; D002413:Cations, Divalent; D002467:Cell Nucleus / Q000201:enzymology*; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D008345:Manganese; D009206:Myocardium / Q000201:enzymology* / Q000648:ultrastructure; D051381:Rats
D000838:Anions; D001639:Bicarbonates; D002413:Cations, Divalent; D008345:Manganese; D000251:Adenosine Triphosphatases
Issledovanie anionchuvstvitel'noĭ ATF-azy iader serdtsa krysy.