Liquid-solid chromatographic determination of 6-demethylgriseofulvin in urine.
A specific and quantitative liquid-solid chromatographic method for the determination of 6-demethylgriseofulvin in human urine is reported. The method consists of extraction into an organic solvent, addition of internal standard, and analysis by liquid-solid chromatography using a UV detector. Griseofulvin, if present, can be determined simultaneously. The sensitivity of the method is 6 mug/ml of urine. Total 6-demethylgrisefulvin is determined after hydrolysis of the glucuronide conjugate with glucuronidase-sulfatase enzyme solution. The method is well suited for the analysis of a large number of samples.
D002851:Chromatography, High Pressure Liquid; D006118:Griseofulvin / Q000031:analogs & derivatives* / Q000652:urine; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008722:Methods
D006118:Griseofulvin