Metabolic N-oxidation of atropine, hyoscine and the corresponding nor-alkaloids by guinea-pig liver microsomal preparations.
Incubation of guinea-pig liver microsomal preparations with atropine or hyoscine resulted in the formation of the corresponding nor-alkaloids and both isomers of atropine N-oxide from atropine and one isomer of hyoscine N-oxide from hyoscine. Separate incubations of guinea-pig liver microsomal preparations with nor-atropine and nor-hyoscine yielded the corresponding hydroxylamines. The N-oxide and hydroxylamine metabolites were identified by comparison of their t.l.c. behavior and m.x. with prepared compounds and also by their reduction to the corresponding tertiary or secondary amines.
D000470:Alkaloids / Q000378:metabolism*; D000818:Animals; D001285:Atropine / Q000378:metabolism*; D003067:Coenzymes / Q000378:metabolism; D006168:Guinea Pigs; D006898:Hydroxylamines / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D010084:Oxidation-Reduction; D012601:Scopolamine / Q000378:metabolism*
D000470:Alkaloids; D003067:Coenzymes; D006898:Hydroxylamines; D001285:Atropine; D012601:Scopolamine