Tris buffer attenuates acetylcholine responses in Aplysia neurons.
The commonly used buffering agent tris(hydroxymethyl)methylamine (tris) antagonizes the action of iontophoretically applied acetylcholine on neurons of Aplysia californica. Concentrations of 5 to 10 millimolar tris markedly reduced both excitatory and inhibitory responses.
D000109:Acetylcholine / Q000008:administration & dosage / Q000037:antagonists & inhibitors*; D000818:Animals; D005724:Ganglia; D006863:Hydrogen-Ion Concentration; D007478:Iontophoresis; D008564:Membrane Potentials / Q000187:drug effects; D008974:Mollusca; D009433:Neural Inhibition / Q000187:drug effects; D009474:Neurons / Q000187:drug effects*; D009435:Synaptic Transmission / Q000187:drug effects; D014325:Tromethamine / Q000494:pharmacology*
D014325:Tromethamine; D000109:Acetylcholine