Spectrophotometric determination of diphenhydramine hydrochloride using dipicrylamine.
A spectrophotometric procedure for the determination of diphenhydramine hydrochloride based on the reaction with dipicrylamine was developed. A yellow complex forms and is easily extractable by chloroform at pH 5. The mole ratio of diphenydramine hydrochloride to dipicrylamine in the complex is 1:3. The absorbance of the complex obeys Beer's law over the concentration range of 3-10 mug of diphenhydramine hydrochloride per ml of chloroform. This procedure can be carried out in the presence of other compounds without interference.
D000814:Aniline Compounds / Q000031:analogs & derivatives; D004155:Diphenhydramine / Q000032:analysis*; D004159:Diphenylamine / Q000031:analogs & derivatives; D004355:Drug Stability; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008722:Methods; D013696:Temperature
D000814:Aniline Compounds; D007202:Indicators and Reagents; D004155:Diphenhydramine; D004159:Diphenylamine