Sterol 24(28) methylene reductase in Saccharomyces cerevisiae.
Optimal conditions for the 24(28)methylene reductase were obtained. The enzyme assay provided for unusually high activity; the Km was determined to be 10.8 mum. The enzyme activity was increased in cells grown with ethanol as the substrate.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D004229:Dithiothreitol / Q000494:pharmacology; D004875:Ergosterol / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009243:NAD / Q000494:pharmacology; D009249:NADP / Q000494:pharmacology; D010088:Oxidoreductases / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D013261:Sterols
D013261:Sterols; D009243:NAD; D009249:NADP; D000255:Adenosine Triphosphate; D010088:Oxidoreductases; C022673:delta 24-sterol reductase; D004229:Dithiothreitol; D004875:Ergosterol