Concentration of poliovirus from tap water onto membrane filters with aluminum chloride at ambient pH levels.
A method is described for the concentration of an enterovirus from large volumes of tap water by addition of small amounts of aluminum chloride to enhance virus removal by membrane filters. Tap water treated with 2 X 10(-5) M aluminum chloride showed a slight decrease in pH (less than 0.5), a slight increase in turbidity, and enhanced removal of poliovirus by membrane filters. Virus was quantitatively recovered by treating the filters with a basic buffer, and this eluate was reconcentrated to a small volume by adsorption to aluminum hydroxide flocs. Using these procedures, virus from 1,000 liters of water was reduced to a final eluate of 20 to 80 ml with a mean recovery of 70%.
D000327:Adsorption; D000535:Aluminum; D002712:Chlorides; D005374:Filtration / Q000295:instrumentation; D006863:Hydrogen-Ion Concentration; D008828:Microbiological Techniques; D017955:Poliovirus / Q000302:isolation & purification*; D014871:Water Microbiology; D014881:Water Supply
D002712:Chlorides; D000535:Aluminum