Use of an iridium electrode for direct measurements of pI of proteins after isoelectric focusing in polyacrylamide gel.
The use of an iridium microelectrode 0.5 mm in diameter is proposed for measuring the pH gradient in polyacrylamide gels after isoelectric focusing. The electrode exhibits a perfectly linear potential/pH relationship; thus it can be used directly in conjunction with a pH meter using the pH scale for readings. pH equilibrium values are rapidly reached (10-15 s) and pI determinations are obtainable with good accuracy (better than 0.1 pH).
D002151:Calorimetry; D005069:Evaluation Studies as Topic; D005782:Gels; D006863:Hydrogen-Ion Concentration; D007495:Iridium; D007525:Isoelectric Focusing / Q000379:methods; D008839:Microelectrodes; D011506:Proteins / Q000302:isolation & purification; D013696:Temperature; D013816:Thermodynamics
D005782:Gels; D011506:Proteins; D007495:Iridium