Isolations in a mosquito (Aedes pseudoscutellaris) cell line (Mos. 61) of yellow fever virus strains from original field material.
A simple, rapid and inexpensive method of isolating yellow fever (YF) virus from naturally infected mosquitoes, human liver and the serum of a sentinel monkey by inoculation of a continuous line of mosquito cells is described. The mosquito cells were more sensitive than suckling mice and marginally better than Vero cells for primary isolation. This is the first time that mosquito cells have been successfully used for primary isolation of YF virus from field material.
D000330:Aedes; D000818:Animals; D001769:Blood / Q000382:microbiology; D002460:Cell Line; D009033:Culicidae / Q000382:microbiology; D003588:Cytopathogenic Effect, Viral; D005069:Evaluation Studies as Topic; D000882:Haplorhini; D006801:Humans; D008099:Liver / Q000382:microbiology; D013045:Species Specificity; D015005:Yellow fever virus / Q000302:isolation & purification*