Preenrichment procedure for detection of Salmonella in gelatin.
Trypticase soy broth was superior to nutrient and lactose broths as a preenrichment medium for the detection of Salmonella in artificially and naturally contaiminated gelatin. The detection rate for Salmonella were further enhanced when homogenization of the gelatin-broth mixture was accomplished by the use of gelatinase rather than by heating of 45 degrees C. Detection rats were also increased by adjusting the pH of the gelatin-broth mixture of 7.0, optimum pH for gelatinase (EC 3.4.23.2) activity.
D003470:Culture Media; D005506:Food Contamination; D005516:Food Microbiology; D005780:Gelatin; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D010434:Pepsin A / Q000378:metabolism; D012475:Salmonella / Q000302:isolation & purification*
D003470:Culture Media; D005780:Gelatin; D010434:Pepsin A