[Isolation of proteins with complex forming agents].
Taking vegetable albumins for models, the authors report of the possibilities of isolating proteins (which cannot be precipitated isoelectrically) by using their property of forming complexes with tannin or poly-anions. The precipitation of proteins with dextran sulphate or polyphosphates, which is due to electrostatic interaction, depends on the pH value and the electrolyte content of the solution. Under appropriate experimental conditions, protein yields of 100% are achieved. By means of tannin, the proteins are completely precipitated in a wide range of pH. The protein component of the poly-anion-containing complexes is isolated by precipitation with salt or by thermal coagulation after dissolving of the complexes. The isolation of protein from the tannin complexes is preferably realized by reaction with coffeine.
D000418:Albumins / Q000302:isolation & purification; D002110:Caffeine / Q000302:isolation & purification; D002850:Chromatography, Gel; D003911:Dextrans; D006368:Helianthus; D006863:Hydrogen-Ion Concentration; D009994:Osmolar Concentration; D010940:Plant Proteins / Q000302:isolation & purification*; D012639:Seeds / Q000032:analysis*; D012965:Sodium Chloride; D013634:Tannins
D000418:Albumins; D003911:Dextrans; D010940:Plant Proteins; D013634:Tannins; D002110:Caffeine; D012965:Sodium Chloride
Proteingewinnung unter Einsatz von komplexbildenden Stoffen