Adjuvant and immunostimulating activities of water-soluble substances extracted from Mycobacterium tuberculosis (var. hominis).
Water-soluble substances have been extracted from two strains of Mycobacterium tuberculosis var. hominis: the native hydrosoluble part (polysaccharide and peptidoglycan), a substance in which the polysaccharide moiety is less abundant than in the latter, the acetylated peptidoglycan and, finally a tetrasaccharide-heptapeptide. All four types of substances, when they were injected together with Freund's incomplete adjuvant, exerted an adjuvant effect on the production of delayed-type hypersensitivity to ovalbumin in the guinea pig and on the production of anti-influenza virus antibodies in the rabbit. Injected intravenously in the mouse, they increased the number of antibody-producing cells in the spleen and enhanced the graft versus host reaction; no effect was seen on the phagocytic activity of the reticulo-endothelial system. By contrast with wax D, the water-soluble substances were devoid of arthritis-inducing activity in the rat. Altogether, these water-soluble substances seem to be endowed with at least some of the adjuvant activities of Freund's complete adjuvant and some of the immunostimulant activities of a live Mycobacterium like BCG.
D000276:Adjuvants, Immunologic / Q000302:isolation & purification*; D000818:Animals; D000914:Antibodies, Viral; D000917:Antibody Formation / Q000187:drug effects; D000921:Antibody-Producing Cells / Q000187:drug effects; D001172:Arthritis, Rheumatoid / Q000139:chemically induced; D006087:Graft vs Host Reaction / Q000187:drug effects; D006168:Guinea Pigs; D006968:Hypersensitivity, Delayed / Q000276:immunology; D051379:Mice; D009169:Mycobacterium tuberculosis / Q000032:analysis*; D009975:Orthomyxoviridae / Q000276:immunology; D010047:Ovalbumin; D010457:Peptidoglycan / Q000302:isolation & purification; D010587:Phagocytosis / Q000187:drug effects; D011134:Polysaccharides / Q000302:isolation & purification; D011817:Rabbits; D012995:Solubility; D013154:Spleen / Q000166:cytology
D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D010457:Peptidoglycan; D011134:Polysaccharides; D010047:Ovalbumin