Studies on groundnut proteins. VI. Isolation, characterisation and hydrogen ion titration of conarachin II.
A method is described for isolating conarachin II in a homogeneous form by the techniques of DEAE-cellulose chromatography, polyacrylamide gel electrophoresis and sedimentation velocity. The protein contains 0.72% carbohydrate and no phosphate. Hydrogen ion titration curve indicated that the sidechain carboxyl, imidazol and epsilon-amino groups titrated with normal pK Int values and their number agreed with the analytical values obtained from amino acid analysis. However, tyrosine phenolic groups had abnormal pK Int of 10.5.
D000596:Amino Acids / Q000032:analysis; D010367:Arachis / Q000032:analysis*; D002241:Carbohydrates / Q000032:analysis; D002846:Chromatography, Affinity; D002848:Chromatography, DEAE-Cellulose; D004591:Electrophoresis, Polyacrylamide Gel; D005916:Globulins / Q000032:analysis / Q000302:isolation & purification; D006859:Hydrogen / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008722:Methods; D010447:Peptide Hydrolases / Q000302:isolation & purification*; D010710:Phosphates / Q000032:analysis; D010758:Phosphorus / Q000032:analysis; D010940:Plant Proteins / Q000032:analysis / Q000302:isolation & purification; D014461:Ultracentrifugation
D000596:Amino Acids; D002241:Carbohydrates; D005916:Globulins; D010710:Phosphates; D010940:Plant Proteins; D010758:Phosphorus; D006859:Hydrogen; D010447:Peptide Hydrolases