[Isolation and properties of 2 kallikreins from human saliva].
Two kallikreins (K-I and K-II) were purified from mixed, parotid and submandibular human saliva. The kallikreins were separated by chromatography on CM-cellulose. The pH optima of activity were at pH 9.3 and pH 9.6-9.8; Km for BAEE was 1.10-3M and 4.10-3M, respectively. The esterase activity of K-I and K-II was inhibited by trasylol-like inhibitors, while the plant and synthetic inhibitors of trypsin were uneffective. In dog, rabbit and rat the hypotensive effect of K-II and its action upon the permeability of rabbit skin was 4-5 fold higher than the effects of K-I. Ki and K-II did not alter the arterial blood pressure in guinea pig.
D000818:Animals; D004285:Dogs; D006168:Guinea Pigs; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007610:Kallikreins / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D009928:Organ Specificity; D010306:Parotid Gland / Q000201:enzymology; D011817:Rabbits; D051381:Rats; D012463:Saliva / Q000201:enzymology*; D013363:Submandibular Gland / Q000201:enzymology
D007610:Kallikreins
Vydelenie i svoĭstva dvukh kallikreinov sliuny cheloveka