[Immobilization of alpha-amylase on porous glass and silochrome].
Immobilized forms of alpha-amylase from Aspergillus oryzae were prepared on the porous glass and silochrome by the glutaraldehyde method. An addition of calcium ions at a concentration of 0.05 M to the reaction mixture during immobilization stabilized the enzyme activity. pH optimum of the insoluble form of alpha-amylase was 5.8 and that of the soluble form was 4.7. Storage of the insoluble enzyme as water suspension in 0.015 M CaCl2 at 4 degrees C for six months and twenty times repeated specific reaction did not affect significantly the activity of insoluble alpha-amylase.
D000681:Amylases / Q000494:pharmacology*; D001236:Aspergillus oryzae / Q000201:enzymology; D004800:Enzymes, Immobilized; D005898:Glass; D006863:Hydrogen-Ion Concentration; D012822:Silicon Dioxide / Q000494:pharmacology*; D012996:Solutions; D000516:alpha-Amylases / Q000494:pharmacology*
D004800:Enzymes, Immobilized; D012996:Solutions; D012822:Silicon Dioxide; D000681:Amylases; D000516:alpha-Amylases
Immobilizatsiia al'fa-amilazy na poristom stekle i silokhrome.