[Ribonuclease activity in preparations of human leukocyte interferon].
Preparations of human leukocyte interferon obtained by multi-stage purification procedure exhibited ribonuclease activity with the optimum at pH 7.0--7.5. The enzyme possessed the endonuclease action mechanism. Most substances studied for their effect on the RNA-ase activity in human interferon preparations showed many of them to act on the enzyme in the same way as on other ribonucleases. However, dithioerythritie, a reducing agent for disulfide bounds, activated the ribonuclease in the interferon preparation, as distinct from the pancreatic ribonuclease, which was inhibited by this preparation. Patterns of protein and RNA-ase distribution were obtained by electrophoresis in polyacrylamide gel.
D002384:Catalysis; D004789:Enzyme Activation; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007372:Interferons / Q000032:analysis*; D012260:Ribonucleases / Q000032:analysis*
D007372:Interferons; D012260:Ribonucleases
Ribonukleaznaia aktivnost' v preparatakh leĭkotsitarnogo interferona cheloveka