Synthesis of soluble dextran-hemoglobin complexes of different molecular sizes.
Experimental conditions were defined that determined the synthesis of dextran-hemoglobin complexes through the alklation of hemoglobin by N-bromoacetylaminoethylaminodextran. Using appropriate concentrations of the two reactants, over 90% yield of dextran-hemoglobin was obtained for dextrans of average molecular weight of 200 000 110000, 70000, 400000, and 20000. Extensive viscosity increase due to crosslinking could be avoided, and a large molar excess of dextran over hemoglobin made unnecessary, under the optimal conditions.
D003911:Dextrans; D006454:Hemoglobins; D006801:Humans; D006863:Hydrogen-Ion Concentration; D046911:Macromolecular Substances; D008970:Molecular Weight; D011485:Protein Binding; D012995:Solubility; D014783:Viscosity
D003911:Dextrans; D006454:Hemoglobins; D046911:Macromolecular Substances