Ultramicromethod for the determination of human arginase in the presence of urea.
A technique for arginase determination in body fluids in the presence of urea is described. [14C]Arginine is hydrolysed by arginase to [14C]urea and ornithine. [14C]Urea is separated with paper chromatography and measured in a liquid scintillation counter. The experimental conditions including the pH, substrate concentration, activator, solvent for chromatography, urea inhibition, and arginase in hemolysates, are discussed.
D001119:Arginase / Q000097:blood / Q000378:metabolism*; D004789:Enzyme Activation; D004912:Erythrocytes / Q000201:enzymology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008345:Manganese / Q000494:pharmacology; D008722:Methods; D008832:Microchemistry; D014508:Urea / Q000494:pharmacology*
D008345:Manganese; D014508:Urea; D001119:Arginase