[pH-dependence characteristics of Ca-ATPase activity of heavy meromyosin with modified SH-groups].
Study of pH-dependence of Ca-ATPase activity of heavy meromyosin (HMM) at low and high ionic strength showed essential differences in the modifying effect of two sulfhydryl reagents, p-CMB and silver. Silver ions in conditions studied independently on pH and KCl concentration produce an inhibition of ATP hydrolysis by myosin and HMM, the shape of the pH-dependence curve remaining similar to that of the native enzyme up to 40% of blocking free sulfhydryl groups. At the same degree of binding of sulfhydryl groups with p-CMB at 0,5 M KCl the pH-dependence curve due to activation at neutral pH changes it's shape and becomes similar to that for dissociation of two ionizable groups (at neutral and alkaline regions). In contrast to this, a low or zero concentrations of KCl no activation was observed for the enzyme with 40-50% of SH-Groups modified by p-CMB and Ca-ATPase in this case seemed to be independent of pH. The data obtained suggest that SH-Groups are not included into the active site of myosin, and the activating effect observed for some sulfhydryl reagents, is due to conformational changes and it can be the result of the penetrance of the organic part of the reagent molecule into hydrophobic region of the protein.
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D001665:Binding Sites; D002118:Calcium; D055598:Chemical Phenomena; D002621:Chemistry; D002729:Chloromercuribenzoates / Q000494:pharmacology; D004789:Enzyme Activation; D006863:Hydrogen-Ion Concentration; D015879:Myosin Subfragments / Q000378:metabolism*; D009994:Osmolar Concentration; D011485:Protein Binding; D011487:Protein Conformation; D012834:Silver / Q000494:pharmacology; D013329:Structure-Activity Relationship; D013438:Sulfhydryl Compounds / Q000378:metabolism
D002729:Chloromercuribenzoates; D015879:Myosin Subfragments; D013438:Sulfhydryl Compounds; D012834:Silver; D000251:Adenosine Triphosphatases; D002118:Calcium
Osobennosti pH-zavisimosti Ca-ATPaznoi aktivnosti tiazhelogo meromiozina s modifitsirovannymi SH-gruppami.