Improved localization of thiamine pyrophosphatase activity in mounted cryostat sections using gel fixation and gel incubation media.
The presence of 1% agar in the fixation and substrate solutions for the histochemical demonstration of thiamine pyrophosphatase (4.4 mM TPP; 3.6 mM Pb2+; 0.025 Tris-maleate buffer, pH 7.2) clearly facilitates the localization of the enzyme in Golgi apparatus in cold microtome sections prepared from unfixed specimens.
D000818:Animals; D002540:Cerebral Cortex / Q000201:enzymology*; D005404:Fixatives; D005782:Gels; D006056:Golgi Apparatus / Q000201:enzymology; D006651:Histocytochemistry / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D011755:Pyrophosphatases / Q000302:isolation & purification*; D051381:Rats; D013834:Thiamine Pyrophosphatase / Q000302:isolation & purification*
D005404:Fixatives; D005782:Gels; D011755:Pyrophosphatases; D013834:Thiamine Pyrophosphatase