The catabolism of L-tyrosine by an Arthrobacter sp.
An Arthrobacter sp. metabolizes L-tyrosine by a pathway involving 3,4-dihydroxyphenylacetate as a key intermediate. p-Hydroxyphenylpyruvate is formed from tyrosine by an amino-transferase specifically requiring alpha-ketoglutarate for activity, and is then converted to p-hydroxyphenylacetate by an oxidative decarboxylation. p-Hydroxyphenylacetaldehyde is not an intermediate in the formation of p-hydroxyphenylacetate. Extracts of the bacterium oxidize 3,4-dihydroxyphenylacetate to delta-carboxymethyl-alpha-hydroxymuconic acid which, when supplemented with 2 mol of diphosphopyridine dinucleotide, results in the production of stoichiometric amounts of succinate and pyruvate.
D015102:3,4-Dihydroxyphenylacetic Acid / Q000378:metabolism; D015111:4-Hydroxyphenylpyruvate Dioxygenase / Q000378:metabolism; D001173:Arthrobacter / Q000201:enzymology / Q000378:metabolism*; D002474:Cell-Free System; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010105:Oxygenases / Q000378:metabolism; D011773:Pyruvates / Q000096:biosynthesis; D013237:Stereoisomerism; D013386:Succinates / Q000096:biosynthesis; D014443:Tyrosine / Q000378:metabolism*; D014444:Tyrosine Transaminase / Q000378:metabolism
D011773:Pyruvates; D013386:Succinates; D009243:NAD; D015102:3,4-Dihydroxyphenylacetic Acid; D014443:Tyrosine; D009249:NADP; D010105:Oxygenases; D015111:4-Hydroxyphenylpyruvate Dioxygenase; D014444:Tyrosine Transaminase