Extraction of tyrosine aminotransferase mRNA by polyribosome immunosorption on sepharose 4B.
Sepharose 4B column with antibody to tyrosine aminotransferase (E.C. 2.6.1.5) (TAT) covalently bound can selectively remove a specific fraction of TAT polysomes from rat liver homogenates. From the polysomes which was adsorbed by immunosorbent one may obtain mRNA containing a segment of poly-adenilate-rich residues, having sedimentation constant of 11--16S. This mRNA may program synthesis of the specific protein in a cell free system. Near 70% of the protein was synthesized in such a system, may react with the antibody to TAT.
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D007163:Immunosorbent Techniques; D008297:Male; D011132:Polyribosomes; D012333:RNA, Messenger / Q000302:isolation & purification*; D051381:Rats; D014444:Tyrosine Transaminase
D012333:RNA, Messenger; D014444:Tyrosine Transaminase
Poluchenie mRNK dlia tirozinaminotransferazy s ispol'zovaniem immunosorbtsii poliribosom na sefaroze 4B.