Cystic fibrosis: isolation and physical properties of a salivary cystic fibrosis factor.
A ciliostatic factor has been isolated from cystic fibrosis (CF) saliva by dialyzing it from purified alpha-amylase prepared by a glycogen-complex method. This method of isolating the CF factor is an improvement over the previously employed heparin procedure. The activity of the isolated factor is proportional with concentration using the oyster gill ciliostatic assay and in its inhibition of mammalian glycogen debranching enzyme. The ciliostatic action of the factor can be reversed by heparin under certain conditions. The type of inhibition of the debranching enzyme by the isolated CF factor indicates that its chemical structure is similar to that observed with hydroxyalkylamines and polyamine metabolites. Physical properties of the isolated factor indicate that it is of low molecular weight and is labile as a function of pH and temperature. At neutral pH the conditions under which it is maintained have a direct effect on the length of time that it is stable.
D000818:Animals; D002923:Cilia / Q000187:drug effects; D003550:Cystic Fibrosis / Q000378:metabolism*; D006003:Glycogen; D006004:Glycogen Debranching Enzyme System / Q000037:antagonists & inhibitors; D006493:Heparin; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008722:Methods; D010125:Ostreidae; D011817:Rabbits; D012463:Saliva / Q000032:analysis*; D012471:Salivary Proteins and Peptides / Q000302:isolation & purification* / Q000494:pharmacology; D013696:Temperature; D000516:alpha-Amylases / Q000302:isolation & purification / Q000494:pharmacology
D006004:Glycogen Debranching Enzyme System; D012471:Salivary Proteins and Peptides; D006493:Heparin; D006003:Glycogen; D000516:alpha-Amylases