Enhanced heat, alkaline and tryptic stability of acetamidinated pig heart lactate dehydrogenase.
Modification of 17 from 24 lysine residues in pig heart lactate dehydrogenase (L-lactate: NAD+ oxidoreductase, EC 1.1.1.27) with methyl aceimidate yields an enzyme derivative with enhanced stability toward meat and alkaline denaturation as well as tryptic digestion. The specific activity of the modified enzyme is only slightly reduced
D000081:Acetamides; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D004355:Drug Stability; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D009206:Myocardium / Q000201:enzymology*; D011487:Protein Conformation; D011489:Protein Denaturation; D013329:Structure-Activity Relationship; D013552:Swine; D014357:Trypsin / Q000378:metabolism
D000081:Acetamides; D007770:L-Lactate Dehydrogenase; D014357:Trypsin