[Genetic independence of two forms of carbonic anhydrase from bovine erythrocytes].
The two major forms of bovine erythrocyte carbonic anhydrase have been designated as CI and CII because their high activity of the C type. Separation of both forms and isolation of CI from the ethanol chloroform extract of the hemolysate were obtained by either column chromatography on DEAE-cellulose DE 23 or on DEAE-sephadex A-50. But pure preparations of the CII form were only obtained from DEAE-sephadex A-50 which separated CII from a minor component CIv1. Comparative studies of the CI and CII forms and of the minor component CIv1 strongly suggest that CIv1 is a conformational variant of CI and CII are genetic variants differing at least in their primary structure by one Arg yields Gln substitution 56 residues from the N-acetylated terminus. Based on the large variability of the proportion of the two isozymes in heterozygous individuals, the modality of the inheritance of these enzymes is discussed.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002256:Carbonic Anhydrases / Q000097:blood*; D002417:Cattle; D003488:Cyanogen Bromide; D004912:Erythrocytes / Q000201:enzymology*; D014644:Genetic Variation; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000097:blood*; D010446:Peptide Fragments / Q000032:analysis
D000596:Amino Acids; D007527:Isoenzymes; D010446:Peptide Fragments; D002256:Carbonic Anhydrases; D003488:Cyanogen Bromide
Indépendance génétique de deux formes de l'anhydrase carbonique érythrocytaire bovine.