Liquid chromatography in pharmaceutical analysis V: determination of an isoniazid-pyridoxine hydrochloride mixture.
Operating parameters are described for the qualitative and quantitative analysis of an isoniazid-pyridoxine hydrochloride mixture by high-pressure liquid chromatography. Each compound was chromatographed on an octadecyl column, using absolute methanol-water (60:40) (pH 2.5) containing 0.01 M dioctyl sodium sulfosuccinate. The flow rate was 2.0 ml/min (2500 psig), and the peaks were detected at 293 nm. The analysis was accomplished using ion-pair formation for effecting chromatographic separation. The time required for separation of the drug mixture is approximately 12 min with an accuracy of 1.17-0.30%.
D002851:Chromatography, High Pressure Liquid; D004338:Drug Combinations; D006863:Hydrogen-Ion Concentration; D007538:Isoniazid / Q000032:analysis*; D008722:Methods; D011736:Pyridoxine / Q000032:analysis*
D004338:Drug Combinations; D011736:Pyridoxine; D007538:Isoniazid