The influence of adrenalectomy on monoamine oxidase and NADH cytochrome c reductase in the rat heart.
The effect of adrenalectomy on the activities of monoamine oxidase (MAO), NADH cytochrome c reductase (NCR), succinate dehydrogenase, malate dehydrogenase, fumarase, NAD+ nucleosidase and acid phosphatase in homogenates of rat hearts was examined. Besides MAO only the NCR activity increased. However, both the total and the rotenone-insensitive NCR activities increased, with that of the rotenone-insensitive being about half of the total, which indicated that the effect of adrenalectomy was exerted on components of this enzyme localized on both the inner and outer membranes of the mitochondrion. The lack of effect on the other enzymes suggests that adrenalectomy has a relatively selective action on MAO and NCR, and does not work by a generalized increase in protein synthesis or by an effect on the FAD cofactor. The MAO increase was seen with a variety of substrates, and was due to a rise in Vmax without change in Km. The response to adrenalectomy in the summer differed from that seen in the winter. The possible reasons for these effects of adrenalectomy are discussed.
D000315:Adrenalectomy; D000818:Animals; D003010:Clorgyline / Q000494:pharmacology; D003579:Cytochrome Reductases / Q000378:metabolism*; D005260:Female; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D008995:Monoamine Oxidase / Q000378:metabolism*; D009206:Myocardium / Q000201:enzymology*; D051381:Rats; D012402:Rotenone / Q000494:pharmacology; D012737:Sex Factors; D014439:Tyramine / Q000378:metabolism
D012402:Rotenone; D008995:Monoamine Oxidase; D003579:Cytochrome Reductases; D003010:Clorgyline; D014439:Tyramine