Cation transport in cytochrome oxidase reconstituted vesicles.
Cation translocation across the membrane of cytochrome oxidase reconstituted vesicles may be followed with a simple spectrophotometric method. Cytochrome oxidase reconstituted vesicles, supplemented with ascorbate and cytochrome c. induce large spectral changes of the positive dye safranine, reversed by uncouplers and inhibitors of respiration. The dye is probably accumulated in the inner space of the vesicles, where it reaches high concentrations and aggregates. The spectral shifts and the absorbance changes, due to aggregation, are proportional to the amount of the dye taken up and depend on the respiratory control. In the presence of potassium, valinomycin causes an inhibition, whereas nigericin stimulates the dye uptake. The data are discussed in terms of electrical potential dependent fluxes.
D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone; D004396:Coloring Agents; D003576:Electron Transport Complex IV / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008567:Membranes, Artificial; D008954:Models, Biological; D009550:Nigericin; D010101:Oxygen Consumption; D010619:Phenazines; D010743:Phospholipids; D011189:Potassium Chloride; D011485:Protein Binding; D013025:Glycine max; D013053:Spectrophotometry; D014634:Valinomycin
D004396:Coloring Agents; D008567:Membranes, Artificial; D010619:Phenazines; D010743:Phospholipids; D014634:Valinomycin; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone; D011189:Potassium Chloride; D003576:Electron Transport Complex IV; D009550:Nigericin; C009195:safranine T