The exchange reaction of peptides R-D-alanyl-D-alanine with D-[14C]alanine to R-D-alanyl-D-[14C]alanine and D-alanine, catalysed by the membranes of Streptococcus faecalis ATCC 9790.
Under alkaline conditions, the membrane-bound DD-carboxypeptidase of Streptococcus faecalis ATCC 9790 catalyses exchange reactions in which the X-L-R3-D-Ala moiety of peptides of the type X-L-R3-D-Ala-D-Ala is transferred to simple amino compounds such as D-alanine, glycine and glycyl-glycine. The enzyme system is unable, however, to catalyse complex reactions that would simulate the natural transpeptidation reaction.
D000409:Alanine / Q000378:metabolism*; D002268:Carboxypeptidases / Q000378:metabolism*; D002462:Cell Membrane / Q000201:enzymology*; D003902:Detergents / Q000494:pharmacology; D013293:Enterococcus faecalis / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D002267:Muramoylpentapeptide Carboxypeptidase / Q000378:metabolism*; D009842:Oligopeptides / Q000378:metabolism*; D009994:Osmolar Concentration; D013329:Structure-Activity Relationship
D003902:Detergents; D009842:Oligopeptides; D002268:Carboxypeptidases; D002267:Muramoylpentapeptide Carboxypeptidase; D000409:Alanine