Measurement of the pH of frozen buffer solutions by using pH indicators.
A method was established to estimate the pH change of several buffers solutions on freezing by using a combination of pH indicators. Among more than 30 buffers solutions examined, almost half exhibited a pH change in the temperature range between freezing point and 220 degrees K; the results were tabulated. Glycerol was found to suppress the pH changes because of its "salt buffer" effect.
D002021:Buffers; D005615:Freezing; D005990:Glycerol; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents
D002021:Buffers; D007202:Indicators and Reagents; D005990:Glycerol