Deoxyribonucleic acid polymerases of Euglena gracilis. Primer-template utilization of and enzyme activities associated with the two deoxyribonucleic acid polymerases of high molecular weight.
The two high-molecular-weight DNA polymerases from Euglena gracilis, pol A (mol. wt. 190 000) and pol B (mol. wt. 240 000), were differentiated on the basis of associated enzymic activities and primer-template utilization. Neither enzyme had endodeoxyribonuclease activity, but pol B, like pol B of yeast and the corresponding enzyme from Tetrahymena pyriformis, exhibited at least one other nuclease activity directed against denatured DNA and the RNA of an RNA-DNA hybrid. These nuclease functions preferred an alkaline pH and Mg2+. Pol B also exhibited nucleoside diphosphokinase activity. Both enzymes were active with 'activated' DNA and poly[d(A-T)] as primer-templates and were sensitive, especially pol B, to inhibition by excess of native or heat-denatured DNA. Pol B also utilized oligo[d(T)] and poly(A) templates under certain conditions, whereas pol A exhibited only slight activity with poly[d(A)]. (U)6 was not used as a primer by either enzyme.
D002474:Cell-Free System; D004247:DNA / Q000378:metabolism; D004254:DNA Nucleotidyltransferases / Q000032:analysis / Q000378:metabolism*; D003851:Deoxyribonucleases / Q000032:analysis; D004720:Endonucleases / Q000378:metabolism; D005056:Euglena gracilis / Q000201:enzymology*; D005092:Exonucleases / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000378:metabolism*; D007700:Kinetics; D008274:Magnesium; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides; D010770:Phosphotransferases / Q000032:analysis / Q000378:metabolism; D011061:Poly A; D011071:Poly T; D011072:Poly U; D012313:RNA / Q000378:metabolism; D013698:Templates, Genetic
D007527:Isoenzymes; D009841:Oligonucleotides; D011061:Poly A; D011071:Poly T; D011072:Poly U; D012313:RNA; D004247:DNA; D010770:Phosphotransferases; D004254:DNA Nucleotidyltransferases; D003851:Deoxyribonucleases; D004720:Endonucleases; D005092:Exonucleases; D008274:Magnesium