The electrophoretic properties and aggregation of mouse lymphoma cells, chinese-hamster fibroblasts and a somatic-cell hybrid.
1. The electrophoretic mobilities of a mouse lymphoma cell, a Chinese-hamster fibroblast and a somatic-cell hybrid (also fibroblastic), produced by fusion of the hamster cell and a mouse lymphoma cell, were measured at 25 degrees C over a range of pH, concentration of Ca2+ ions and concentration of La3+ ions. 2. All the cells have pI at pH3.5. 3. Ca2+ ions decrease the mobilities and zeta potentials of the cells to zero in the range 1-100mM. 4. La3+ ions lower the mobilities and zeta potentials in the range 10 muM-1 mM, and the cells become positively charged above 1 mM. 5. The data are consistent with specific adsorption of La3+ ions on approx. 2 X 10(14) sites/m2 of cell surface with a free energy of approx. -37kJ/mol. 6. The effects of Ca2+, La3+ and ionic strength on the extent of aggregation of the cells and of neuraminidase-treated cells were studied. 7. Ca2+ ions do not markedly increase aggregation, whereas La3+ ions gave rise to extensive aggregation in the range 10 muM-1 mM, corresponding to the region of La3+ adsorption. 8. Both fibroblastic cell lines are aggregated at high ionic strength. 9. The fibroblastic cells have larger amounts of trypsin-sensitive carbohydrate than does the lymphoma cell; the possible role of this material in cellular aggregation is discussed.
D001665:Binding Sites; D002118:Calcium / Q000494:pharmacology; D002449:Cell Aggregation; D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology; D002465:Cell Movement; D004586:Electrophoresis; D005347:Fibroblasts; D006822:Hybrid Cells; D006863:Hydrogen-Ion Concentration; D007811:Lanthanum / Q000494:pharmacology; D008223:Lymphoma; D046911:Macromolecular Substances; D013499:Surface Properties; D014357:Trypsin / Q000494:pharmacology
D046911:Macromolecular Substances; D007811:Lanthanum; D014357:Trypsin; D002118:Calcium