Pig plasma benzylamine oxidase: some considerations on the mechanism of the reaction.
The kinetics of benzaldehyde formation during the oxidation of benzylamine by pig plasma benzylamine oxidase have been studied at different pH values. It has been shown that the first step of the reaction catalyzed by this enzyme is the formation of a Schiff base between the amino group of the substrate and the aldehyde group of the enzyme. Present kinetic studies are consistent with this mechanism and demonstrate the existence of two steps which are alternatively rate-limiting one below pH 6.0, the other above this pH value. The rate-limiting step above pH 6.0 requires the participation of OH-. A mechanism of reaction has been proposed in which the release of products follows the sequence: hydrogen peroxide, aldehyde, ammonia.
D000818:Animals; D001595:Benzylamine Oxidase / Q000097:blood*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008995:Monoamine Oxidase / Q000097:blood*; D013552:Swine
D001595:Benzylamine Oxidase; D008995:Monoamine Oxidase