Interaction of DNA with DNA-binding proteins. The characterization of protein HD from Escherichia coli and its nucleic acid complexes.
DNA-binding protein HD with a monomer molecular weight of 9000 was isolated from Escherichia coli cells. The protein occurs as a tetramer under native conditions and binds to single and double-stranded DNA and also to RNA. DNA complexed with protein HD is a poor template for DNA synthesis by E. coli polymerase I, II or III holoenzyme. Exonuclease III is hindered in degrading HD-protein-covered double-stranded DNA, whereas exonuclease I can digest complexed single-stranded DNA. Transcription is liqhtly stimulated in the presence of protein HD.
D002352:Carrier Proteins / Q000032:analysis* / Q000378:metabolism; D004269:DNA, Bacterial / Q000096:biosynthesis / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D005092:Exonucleases / Q000037:antagonists & inhibitors; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D019384:Nucleic Acid Synthesis Inhibitors; D011485:Protein Binding; D011487:Protein Conformation; D012329:RNA, Bacterial / Q000378:metabolism; D014158:Transcription, Genetic
D002352:Carrier Proteins; D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D019384:Nucleic Acid Synthesis Inhibitors; D012329:RNA, Bacterial; D005092:Exonucleases