Isolation and properties of muscular glucose dehydrogenase (E.C. 1.1.1.47) from the stomachs of geese and ducks.
A method which purified 30-fold perparations of muscular glucose dehydrogenase from the stomachs of geese and ducks was elaborated. Activity of this enzyme was found to be dependent on presence of free-SH groups, NAD or NADP as coenzymes, and had an optimum at about pH 7-8. The enzyme's activity against 17 different monosaccharides and their derivatives was tested. The best substrate was beta-D-glucose, followed by D-glucose, D-glucosamine and D-xylose. Other compounds such as D-glucose-6-phosphate, D-fructose, D-mannose and 2-deoxyglucose were not oxidized. The enzyme preparation showed presence of enzymes which conduct gluconic acid metabolism.
D002731:4-Chloromercuribenzenesulfonate / Q000494:pharmacology; D000429:Alcohol Oxidoreductases / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D050260:Carbohydrate Metabolism; D004372:Ducks / Q000378:metabolism*; D004492:Edetic Acid / Q000494:pharmacology; D005777:Geese / Q000378:metabolism*; D005947:Glucose; D006863:Hydrogen-Ion Concentration; D009130:Muscle, Smooth / Q000201:enzymology*; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D013270:Stomach / Q000201:enzymology*; D013329:Structure-Activity Relationship
D009243:NAD; D009249:NADP; D002731:4-Chloromercuribenzenesulfonate; D004492:Edetic Acid; D000429:Alcohol Oxidoreductases; D005947:Glucose