Characterization and induction of aryl hydrocarbon (benzo(a)pyrene) hydroxylase in rabbit bone marrow.
Benzo(a)pyrene hydroxylase activity was found in the bone marrow of control and 3MC-induced New Zealand white rabbits. This activity was localized in the microsomal fraction, was NADPH dependent and CO sensitive. The reaction was inhibited by 7,8-benzoflavone indicating that it was mediated by the 3MC-inducible form of cytochrome P-450. BP hydroxylase activity in rabbit bone marrow was located primarily in white cells, and was considerably higher than that previously reported for cultured human lymphocytes and monocytes. A possible role for the bone marrow mixed-function oxygenase in the production of hemopoietic toxicity is considered.
D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000032:analysis; D001579:Benzopyrene Hydroxylase / Q000032:analysis / Q000378:metabolism; D001853:Bone Marrow / Q000201:enzymology* / Q000648:ultrastructure; D004790:Enzyme Induction / Q000187:drug effects; D005260:Female; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008099:Liver / Q000201:enzymology; D008748:Methylcholanthrene / Q000494:pharmacology; D011817:Rabbits; D013347:Subcellular Fractions / Q000201:enzymology
D008748:Methylcholanthrene; D001579:Benzopyrene Hydroxylase; D001189:Aryl Hydrocarbon Hydroxylases