The tricarboxylic and acid pathway in Desulfovibrio.
Strains of two species of Desulfovibrio were examined for enzymes of the tricarboxylic acid cycle and related pathways. Pyruvate carboxylase (EC6.4.1.1) is present, and alpha-ketoglutarate is formed via the tricarboxylic acids. Glutamate, but not succinyl-CoA, arises from alpha-ketoglutarate. A pathway exists from pyruvate by malic enzyme (EC 1.1.1.39) activity to malate, then fumarate and succinate, again with no evidence of succinyl-CoA formation. The enzymes concerned with metabolism of these dicarboxylic acids show greater activity in the strains that can grow by fumarate dismutation. Glutamate (or glutamine), alpha-ketoglutarate, and yeast extract repress the enzymes that metabolize the tricarboxylic acids. There appears to be no glyoxylate cycle in Desulfovibrio vulgaris or D. desulfuricans.
D000693:Anaerobiosis; D002474:Cell-Free System; D002952:Citric Acid Cycle; D003901:Desulfovibrio / Q000201:enzymology* / Q000378:metabolism; D004794:Enzyme Repression; D005650:Fumarates / Q000378:metabolism; D005971:Glutamates / Q000096:biosynthesis; D007656:Ketoglutaric Acids / Q000378:metabolism; D007773:Lactates / Q000378:metabolism; D009249:NADP / Q000378:metabolism
D005650:Fumarates; D005971:Glutamates; D007656:Ketoglutaric Acids; D007773:Lactates; D009249:NADP