[Formation and purification of the oxidoreductase inhibitor from NAD (AUTHOR'S TRANSL)].
The oxidoreductase inhibitor is not formed from NADH as previously thought, but only from NAD under alkaline conditions. Analogues of NAD (e.g. NADP) and components of the NAD molecule (e.g. ADP) have no effect on the formation of the inhibitor. The most favourable pH, temperature, duration of incubation, type of buffer and NAD concentration for the formation of the inhibitor were investigated. The method for the formation and chromatographic isolation of the oxidoreductase inhibitor is briefly described.
D000244:Adenosine Diphosphate / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009243:NAD / Q000302:isolation & purification / Q000494:pharmacology*; D009247:NADH, NADPH Oxidoreductases / Q000037:antagonists & inhibitors*; D009249:NADP / Q000494:pharmacology; D013696:Temperature
D009243:NAD; D009249:NADP; D000244:Adenosine Diphosphate; D009247:NADH, NADPH Oxidoreductases
Bildung und Reinigung des Oxidoreductase-Inhibitors aus dem NAD