Influence of reagent quality and reaction conditions on the determination of serum albumin by the bromcresol green dye-binding method.
In an age of reference methodologies and sophisticated quality control techniques it is surprising that so little attention is paid to the quality of the reagents that are used. This paper reports on an investigation of the bromcresol green dye-binding procedure for the estimation of serum albumin in which particular attention has been paid to changes in the reagent components that contribute to the performance of the method. It has been shown that detailed attention to the quality control of the reagents can improve the precision and interlaboratory comparison in the estimation of serum albumin.
D001961:Bromcresol Green; D002021:Buffers; D002951:Citrates; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008722:Methods; D011786:Quality Control; D012709:Serum Albumin / Q000032:analysis*; D012710:Serum Albumin, Bovine / Q000032:analysis; D013053:Spectrophotometry; D013386:Succinates; D013501:Surface-Active Agents
D002021:Buffers; D002951:Citrates; D007202:Indicators and Reagents; D012709:Serum Albumin; D013386:Succinates; D013501:Surface-Active Agents; D012710:Serum Albumin, Bovine; D001961:Bromcresol Green