Tritiated thymidine incorporation and cell-mediated lympholysis as correlates of acute graft-versus-host reaction.
The graft-versus-host (GVH) reaction remains a serious consequence of administration of allogeneic immunocompetent cells to an immunosuppressed host even if donors and recipients are matched for major histocompatibility loci. This report describes a murine model for acute GVH reactions. Spleen cells from C3H/He (H-2k) mice, after intravenous injection of BALB/c (H-2d) spleen cells, were specifically cytotoxic for C3H target cells in vitro 4 days after irradiation and reconstitution. The cells in the recipients apparently are of donor genotype. The spleen cells exhibited rapid proliferation in vitro as measured by the uptake of 3H-TdR. The in vitro proliferation was distinguished from erythropoiesis by an assay of 59Fe incorporation. The kinetics of the in vitro incorporation of 3H-TdR and the in vivo uptake of 59Fe are reported.
D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D004920:Erythropoiesis; D006087:Graft vs Host Reaction; D006650:Histocompatibility Testing; D007275:Injections, Intravenous; D007504:Iron Radioisotopes; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008808:Mice, Inbred CBA; D011859:Radiography; D013154:Spleen / Q000166:cytology* / Q000000981:diagnostic imaging / Q000637:transplantation; D013936:Thymidine / Q000378:metabolism*; D014184:Transplantation, Homologous; D014185:Transplantation, Isogeneic
D007504:Iron Radioisotopes; D013936:Thymidine