[Purification and properties of the serotonin-stimulated adenylate deaminase from the mitochondrial fraction of rat liver].
A method is described for partial purification of structurally bound adenilate desaminase from rat liver tissue mitochondria; the enzyme was stimulated by parenteral administration of serotonine. The enzymatic preparations obtained desaminated AMP, 2',3'-AMP and adenosine, but they did not effect on ATP, 2',3'-cycloAMP or 3',5'-cycloAMP. The maximal rate of desaminating of these substances by AMP-desaminase, stimulated with serotonine, exceeded approximately 1.4-fold the same values, which were obtained for the enzymatic preparations from liver tissue mitochondria of rats, administered with physiological solution. Mitochondrial serotomine-stimulated adenilate desaminase was differentiated from the other soluble adenilate desaminases by some properties; the enzyme was likely to participate also in the regulation of nucleotides balance in the organism.
D000659:AMP Deaminase / Q000037:antagonists & inhibitors / Q000302:isolation & purification* / Q000378:metabolism; D000818:Animals; D003641:Deamination; D004789:Enzyme Activation / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D008297:Male; D008722:Methods; D008930:Mitochondria, Liver / Q000187:drug effects / Q000201:enzymology*; D009705:Nucleosides / Q000378:metabolism; D009709:Nucleotide Deaminases / Q000302:isolation & purification*; D009711:Nucleotides / Q000378:metabolism; D051381:Rats; D012701:Serotonin / Q000494:pharmacology*; D013329:Structure-Activity Relationship
D009705:Nucleosides; D009711:Nucleotides; D012701:Serotonin; D009709:Nucleotide Deaminases; D000659:AMP Deaminase
Ob ochistke i svoĭstvakh stimuliruemoĭ serotoninom adenilatdezaminazy mitokhondrial'noĭ fraktsii pecheni krysy