Fluorescence of oxidized flavoproteins from perifused isolated pancreatic islets.
In perifused pancreatic islets, the fluorescence of oxidized flavoproteins (FAD) was recorded continuously. Elevation of glucose concentration in the medium form 0 or 5 mM to 20 mM led to decrease in FAD-fluorescence beginning 10 sec after change of medium. L-leucine (10 mM), (+/-)-B-BCH (20 mM) and alpha-ketoisocaproic acid (10 mM) caused typical kinetics of FAD-fluorescence decrease. The results are interpreted to indicate rapid changes of the functional state of B-cell mitochondria induced by the above-mentioned stimulators of insulin release.
D000596:Amino Acids / Q000494:pharmacology; D000598:Amino Acids, Cyclic; D000818:Animals; D001120:Arginine / Q000494:pharmacology; D002208:Caproates / Q000494:pharmacology; D005260:Female; D005182:Flavin-Adenine Dinucleotide / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology; D007515:Islets of Langerhans / Q000378:metabolism*; D007651:Keto Acids / Q000494:pharmacology; D007930:Leucine / Q000494:pharmacology; D008297:Male; D051379:Mice; D008820:Mice, Obese; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D009636:Norbornanes / Q000494:pharmacology; D010084:Oxidation-Reduction; D010424:Pentobarbital / Q000494:pharmacology
D000596:Amino Acids; D000598:Amino Acids, Cyclic; D002208:Caproates; D007651:Keto Acids; D009636:Norbornanes; D009243:NAD; D005182:Flavin-Adenine Dinucleotide; C006504:2-aminobicyclo(2,2,1)heptane-2-carboxylic acid; D009249:NADP; D001120:Arginine; D007930:Leucine; D010424:Pentobarbital; D005947:Glucose