[Amino acid-p-nitroanilide-splitting activities in human serum (author's transl)].
Proteolytic, arylamidase-like activities of human serum are determined by using a sensitive micromethod, based upon the Bratton-Marshall-reaction, and amino acid-p-nitroanilides as substrates. Besides the alanine-aminopeptidase and the oxytocinase, the dipeptide-aminopeptidase II, a neutral peptidase, and 6 other peptidases, not yet identified by us, are differentiated with the aid of 19 substrates, by adding 8 effectors, and by incubating the enzyme tests within the pH range 6.0 to 8.4.
D000596:Amino Acids / Q000378:metabolism*; D000626:Aminopeptidases / Q000097:blood; D000813:Anilides / Q000378:metabolism*; D002021:Buffers; D010450:Endopeptidases / Q000097:blood; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D008722:Methods; D010447:Peptide Hydrolases / Q000097:blood*
D000596:Amino Acids; D000813:Anilides; D002021:Buffers; D010450:Endopeptidases; D010447:Peptide Hydrolases; D000626:Aminopeptidases
Aminosäure-p-nitroanilide spaltende Aktivitäten im Humanserum.