Glycoprotein biosynthesis in splenic cells. Purification of a microsomal galactosyl-transferase.
One part of the microsomal galactosyl-transferase activity of splenic cells of rats can by solubilized by the action of Triton X-100 and Tween 20. Its purification on a Sephadex G-200 column and by electrophoresis on a polyacrylamide gel leads to a solution of high specific enzymic activity.
D000818:Animals; D005700:Galactosyltransferases / Q000302:isolation & purification / Q000378:metabolism*; D006023:Glycoproteins / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008861:Microsomes / Q000201:enzymology; D011092:Polyethylene Glycols; D011136:Polysorbates; D051381:Rats; D013154:Spleen / Q000201:enzymology*; D013696:Temperature
D006023:Glycoproteins; D011136:Polysorbates; D011092:Polyethylene Glycols; D005700:Galactosyltransferases