Improved methods of influenza virus propagation. I. Enhancement of virus reproduction in cell cultures.
Comparative studies on the reproduction of a set of influenza A and B virus strains in different cell cultures were carried out. A cultivation system yielding influenza virus with high infectivity, based on the use of roller cultures of bovine embryo kidney cells, of virus strains adapted to this culture, and of a maintenance medium of improved composition, was developed.
D000818:Animals; D001120:Arginine; D002417:Cattle; D003470:Culture Media; D046508:Culture Techniques; D006639:Histidine; D006801:Humans; D009975:Orthomyxoviridae / Q000254:growth & development*; D011784:Quail; D014131:Trace Elements; D014776:Virus Cultivation / Q000379:methods*; D014779:Virus Replication
D003470:Culture Media; D014131:Trace Elements; D006639:Histidine; D001120:Arginine