CO2-mediated control of fatty acid metabolism in isolated hamster brown-fat cells during norepinephrine stimulation.
1. Addition of norepinephrine to isolated hamster brown-fat cells suspended in Krebs-Ringer phosphate buffer induces a pronounced, but temporary increase in respiratory rate. 2. If Krebs-Ringer phosphate buffer is bubbled with CO2 prior to the addition of cells and norepinephrine, the respiratory capacity of the cells is further potentiated and most important, the respiration is maintained at a high rate until the medium becomes depleted of oxygen. 3. This respiratory pattern cannot be obtained in CO2-bubbled Krebs-Ringer bicarbonate buffer. 4. The results indicate that CO2 has a regulatory effect on fatty acid metabolism in isolated hamster brown-fat cells.
D000244:Adenosine Diphosphate / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D002001:Adipose Tissue, Brown / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D002245:Carbon Dioxide / Q000494:pharmacology*; D006224:Cricetinae; D000242:Cyclic AMP / Q000378:metabolism; D005230:Fatty Acids, Nonesterified / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008053:Lipid Mobilization / Q000187:drug effects; D008647:Mesocricetus; D009638:Norepinephrine / Q000494:pharmacology*; D010101:Oxygen Consumption / Q000187:drug effects
D005230:Fatty Acids, Nonesterified; D002245:Carbon Dioxide; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D000242:Cyclic AMP; D009638:Norepinephrine