The phosphoenol-pyruvate branchpoint in adult Hymenolepis diminuta (Cestoda): a study of pyruvate kinase and phosphoenol-pyruvate carboxykinase.
The properties of pyruvate kinase (PK) and phosphoenol pyruvate carboxykinase (PEP CK), two enzymes that determine the preferrential accumulation of either succinate or lactate as endproducts of carbohydrate metabolism, are described in adult Hymenolepis diminuta. PK activity at Vmax and Km levels of PEP was unaffected by ATP, alanine, FDP4, OR H+ ions, but was inhibited by 50% at 6.3 mM L-lactate and 30 mM HCO3. The addition of 30 mM HCO3 increased the Km(PEP) by 6-fold but did not alter the Vmax. The inhibition of PK by HCO3 cannot be explained entirely by an effect of ionic strength, but probably represents a specific modulator-enzyme interaction. Under similar conditions PEP CK was maximally activated. Although L-lactate inhibited PEP CK (Ki(lac) = 1.8 mM), this effector may play a minor role in regulation of PEP flux. These results implicate the poise of the HCO3-:CO2 system as a major determiner  of endproduct accumulation in H. diminuta.
D000818:Animals; D001639:Bicarbonates / Q000494:pharmacology; D002021:Buffers; D002245:Carbon Dioxide / Q000494:pharmacology; D002589:Cestoda / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006926:Hymenolepis / Q000201:enzymology / Q000378:metabolism*; D007700:Kinetics; D007773:Lactates / Q000494:pharmacology; D009994:Osmolar Concentration; D010728:Phosphoenolpyruvate / Q000378:metabolism*; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011685:Purine Nucleotides / Q000378:metabolism; D011770:Pyruvate Kinase / Q000037:antagonists & inhibitors / Q000378:metabolism*
D001639:Bicarbonates; D002021:Buffers; D007773:Lactates; D011685:Purine Nucleotides; D002245:Carbon Dioxide; D010728:Phosphoenolpyruvate; D011770:Pyruvate Kinase; D010729:Phosphoenolpyruvate Carboxykinase (GTP)