Assessment of a diagnostic dip-stick for assaying plasma or serum pseudocholinesterase activity.
A diagnostic dip-stick for measuring pseudocholinesterase activity has been assessed by 4 participants and compared with a standard spectrophotometric method. Duplicate dip-stick estimations (measured in kU/l) corresponded closely, and concurrence between participants was good. The dip-stick estimate of cholinesterase was consistently lower than the spectrophotometric value; the magnitude of this deviation depended on the absolute enzyme activity. With enzyme activities of up to 1 kU/l the dip-stick value was 1 unit lower. Between 1 and 4 kU/l the dip-stick estimate compared well with the spectrophotometric value. From 5 to 7 kU/l and beyond 7 kU/l the negative deviation recorded by the dip-stick increased to 2 units or more. Normal enzyme activity is 3 - 6 kU/l. Over the 1 - 4 kU/l range the method is most accurate, thus negative deviation below 1 kU/l or above 4 kU/l is generally diagnostically irrelevant. On the basis of cost, convenience, speed and accuracy this method will suffice in the absence of sophisticated assay facilities.
D001049:Apnea / Q000139:chemically induced; D001774:Blood Chemical Analysis / Q000592:standards; D002091:Butyrylcholinesterase / Q000097:blood*; D002219:Carbamates; D002800:Cholinesterase Inhibitors / Q000506:poisoning; D002802:Cholinesterases / Q000097:blood*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007306:Insecticides / Q000506:poisoning*; D009784:Occupational Diseases / Q000175:diagnosis; D009787:Occupational Medicine; D009943:Organophosphorus Compounds; D011933:Reagent Kits, Diagnostic; D013053:Spectrophotometry; D013390:Succinylcholine / Q000009:adverse effects
D002219:Carbamates; D002800:Cholinesterase Inhibitors; D007306:Insecticides; D009943:Organophosphorus Compounds; D011933:Reagent Kits, Diagnostic; D002091:Butyrylcholinesterase; D002802:Cholinesterases; D013390:Succinylcholine