Gaucher's disease. Factors affecting the 4-methylum-belliferyl-beta-D-glucosidase activity of cultured skin fibroblasts.
Phosphate-buffered saline was found to inhibit acid beta-glucosidase activity of cultured cells. This effect was attributable to both the pH and the chloride content of the phosphate-buffered saline. Washing the cells in sucrose (0.25M) to remove residual phosphate-buffered saline effectively eliminated this inhibition.
D002021:Buffers; D002478:Cells, Cultured; D002712:Chlorides / Q000494:pharmacology; D005347:Fibroblasts / Q000201:enzymology; D005776:Gaucher Disease / Q000201:enzymology*; D005959:Glucosidases / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010710:Phosphates; D012867:Skin / Q000201:enzymology; D012965:Sodium Chloride / Q000494:pharmacology; D013395:Sucrose / Q000494:pharmacology; D001617:beta-Glucosidase / Q000378:metabolism*
D002021:Buffers; D002712:Chlorides; D010710:Phosphates; D012965:Sodium Chloride; D013395:Sucrose; D005959:Glucosidases; D001617:beta-Glucosidase