[Separation of metanephrine and normetanephrine from urine for automated fluorimetric routine determination (author's transl)].
It is possible to separate metanephrine and normetanephrine from urine for the automated fluorimetric routine determination by means of a combination of liquid-liquid partition with ethylacetate and clean-up of the extract through Amberlite XAD-4. The differentiated fluorimetric determination is based on the oxidation with potassium hexacyanoferrate (III) in the presence of zinc ions and different pH values in an autoanalyzer. No quenching effects were observed. The recovery yields were in the range of 60%, the relative standard deviation being less than 10%.
D001322:Autoanalysis; D002852:Chromatography, Ion Exchange / Q000379:methods; D004837:Epinephrine / Q000031:analogs & derivatives*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008676:Metanephrine / Q000302:isolation & purification / Q000652:urine*; D009647:Normetanephrine / Q000302:isolation & purification / Q000652:urine*; D013050:Spectrometry, Fluorescence / Q000379:methods; D015032:Zinc
D009647:Normetanephrine; D008676:Metanephrine; D015032:Zinc; D004837:Epinephrine
Abtrennung von Metanephrin und Normetanephrin aus Urin zur automatisch-fluorimetrischen Bestimmung