Characterization of cell wall polymers secreted into the growth medium of lysis-defective pneumococci during treatment with penicillin and other inhibitors of cell wall synthesis.
Autolysin-defective pneumococci secrete large quantities of choline-containing cell wall polymers into the growth medium during treatment with inhibitors of peptidoglycan synthesis. The secreted polymers were separated into three fractions by a combination of gel filtration on agarose and sodium dodecyl sulfate-gel electrophoresis. Fraction I had a high apparent molecular size and contained the Forssman antigen in complex with material exhibiting properties of cell wall teichoic acid. Choline-containing polymers of as yet uncharacterized structure were present in both fractions IIA and IIB, and fraction IIA also contained peptidoglycan components.
D000907:Antibodies, Bacterial; D002473:Cell Wall / Q000187:drug effects / Q000378:metabolism*; D003523:Cycloserine / Q000494:pharmacology; D010406:Penicillins / Q000494:pharmacology*; D011108:Polymers / Q000032:analysis*; D011135:Polysaccharides, Bacterial / Q000276:immunology; D013296:Streptococcus pneumoniae / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure
D000907:Antibodies, Bacterial; D010406:Penicillins; D011108:Polymers; D011135:Polysaccharides, Bacterial; D003523:Cycloserine