[Specific p-nitrophenyl phosphatase of yeast Pichia guilliermondii].
The cells of yeast P. guilliermondii contain specific p-nitrophenyl phosphatase (pNPPase), the level of which depends on the cells supply with inorganic phosphorus. Partially purified enzyme is activated by ions Mg2+, Co2+ and somewhat weaker -- by ions Fe2+. With the presence of Mg2+ the enzyme activity is inhibited by ions Cd2+, Zn2+, f-, Be2+, Cu2+, Mn2+, Ca2+, MoO42-, Fe3+, Fe2+, inorganic phosphate as well as by EDTA. A mixture ions Be2+ and F- causes a complete inhibition of the activity. Ions K+ and Na+ inhibit to some extent the enzymic activity, ATP removes the inhibitory effect of monovalent cations. Km of pNPPase is equal to 3.3-10(-4) M, the molecular weight determined by the method of gelfiltration is 60 000. The enzyme is the most active at 50 degrees C and pH 9,5 PNPPase does not manifest the phosphotranspherase activity in tris-HC1-buffer.
D009597:4-Nitrophenylphosphatase / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000494:pharmacology; D001203:Ascomycota / Q000201:enzymology*; D002413:Cations, Divalent; D003035:Cobalt / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000494:pharmacology; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D008970:Molecular Weight; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D010758:Phosphorus / Q000494:pharmacology; D010843:Pichia / Q000187:drug effects / Q000201:enzymology*; D011188:Potassium / Q000494:pharmacology; D012964:Sodium / Q000494:pharmacology
D002413:Cations, Divalent; D010758:Phosphorus; D003035:Cobalt; D000255:Adenosine Triphosphate; D012964:Sodium; D007501:Iron; D010744:Phosphoric Monoester Hydrolases; D009597:4-Nitrophenylphosphatase; D008274:Magnesium; D011188:Potassium