Properties of deoxynucleoside 5'-monophosphatase induced by bacteriophage T5 after infection of Escherichia coli.
Bacteriophage T5 induced a deoxynucleoside 5'-monophosphatase during its infection of Escherichia coli. The enzyme was purified about 100-fold. It was clearly distinct from the host 5'-nucleotidase activity in its physical characteristics and substrate specificity. The enzyme was active on deoxynucleoside 5'-monophosphates but was not active as a phosphatase on ribonucleotides, deoxynucleoside 5'-triphosphates, deoxynucleoside 3'-monophosphates, or deoxyoligonucleotides. Furthermore, it did not have oligonucleotidase or exonuclease activity. The enzyme could exist in multimeric form but had a monomer molecular weight of about 25,000.
D003090:Coliphages / Q000201:enzymology*; D003854:Deoxyribonucleotides; D004790:Enzyme Induction; D004926:Escherichia coli; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D008970:Molecular Weight; D009708:Nucleotidases / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism*; D013696:Temperature
D003854:Deoxyribonucleotides; D009708:Nucleotidases; D008274:Magnesium