Histamine metabolism. I. Thin-layer radiochromatographic assays for histaminase and histidine decarboxylase enzyme activities.
Thin-layer radiochromatographic methods for the measurement of histaminase and histidine decarboxylase activities have been developed. The assays are specific for the respective enzymes, are sensitive and reproducible, and can be performed using commercially available substrates. The histaminase assay permits determination of enzyme activity from 2.5 mul of pregnancy sera, 1-2 X 10(6) human granulocytes, and microgram quantities of partially purified human placenta histaminase with an error of less than 5 per cent. The histidine decarboxylase assay permits measurement of nanogram quantities of newly formed histamine from as few as 2 X 10(4) rat peritoneal mast cells or rat basophilic leukemia cells with an error of less than 5 per cent.
D006631:Amine Oxidase (Copper-Containing) / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D002250:Carbon Radioisotopes; D002262:Carboxy-Lyases / Q000378:metabolism*; D002855:Chromatography, Thin Layer / Q000379:methods*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D006098:Granulocytes / Q000201:enzymology; D006632:Histamine / Q000378:metabolism*; D006640:Histidine Decarboxylase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008407:Mast Cells / Q000201:enzymology; D009994:Osmolar Concentration; D011247:Pregnancy; D051381:Rats; D013696:Temperature
D002250:Carbon Radioisotopes; D004791:Enzyme Inhibitors; D006632:Histamine; D006631:Amine Oxidase (Copper-Containing); D002262:Carboxy-Lyases; D006640:Histidine Decarboxylase