[Purification and properties of the NADP-specific glutamate dehydrogenase of Candida tropicalis feed yeasts].
From the cell-free extract of fodder yeast Candida tropicalis NADP-specific glutamate dehydrogenase was isolated and partially purified (75-fold) by means of fractional precipitation by ammonium sulphate and ion-exchange chromatography on DEAE-cellulose. The preparation was investigated with the aid of polyacrylamide gel electrophoresis. Kinetic characteristics of the enzyme in the cell-free extract and partially purified preparation were derived.
D000821:Animal Feed; D002175:Candida / Q000201:enzymology*; D002474:Cell-Free System; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation; D005969:Glutamate Dehydrogenase / Q000302:isolation & purification* / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009249:NADP
D009249:NADP; D005969:Glutamate Dehydrogenase
Ochistka i nekotorye svoĭstva NADF-spetsifichnoĭ glutamatdegidrogenazy kormovykh drozhzheĭ Candida tropicalis.