Purification of free polyhedrosis virus infecting Galleria mellonella L.
Free Galleria mellonella nuclear polyhedrosis virus was purified 4000--4500 times by Tween-80 treatment and repeated sucrose density gradient centrifugation, based on the ratio of infectivity to protein content as compared to the starting virus-containing suspension.
D000818:Animals; D002251:Carbon Tetrachloride; D002499:Centrifugation, Density Gradient; D007304:Insect Viruses / Q000302:isolation & purification*; D007814:Larva / Q000382:microbiology; D007915:Lepidoptera / Q000382:microbiology*; D009036:Moths / Q000382:microbiology*; D011136:Polysorbates
D011136:Polysorbates; D002251:Carbon Tetrachloride