Isolation of myosin from starfish sperm heads.
Myosin was extracted and partially purified from the head portion of spermatozoa of the starfish, Asterias amurensis. The sperm myosin showed a specific Ca2+-activated ATPase [EC 3.6.1.3] activity of 0.2 mumoles Pi/min/mg at high ionic strength and pH 6.5. It resembled egg myosin in forming thick filaments, becoming attached to actin filaments. subunit composition, and serological properties.
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D004789:Enzyme Activation; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D008854:Microscopy, Electron; D009218:Myosins / Q000302:isolation & purification / Q000378:metabolism; D009994:Osmolar Concentration; D013077:Sperm Head / Q000032:analysis*; D013094:Spermatozoa / Q000032:analysis*; D013215:Starfish
D000251:Adenosine Triphosphatases; D009218:Myosins; D008274:Magnesium; D002118:Calcium