[A method of continuous recording on microsamples of the Hb-O2 association curve. II. A study of Bohr effect and carbamino-formation (author's transl)].
The authors have worked out an adaptation to microsamples (200-400 mul) of the DUVELLEROY et al. method, allowing the continous registration of the O2-Hb association curve. The microsample being diluted in buffer solution, it is possible to predetermine its pH and PCO2. The prefixed conditions are maintained during the initial deoxygenation phase, and all along the curve registration. Moreover, the adjustment to the desired values of the pH and PCO2 allows the quantification of total BOHR effect, proton BOHR effect and carbamino-formation, during the course of oxygenation.
D002245:Carbon Dioxide / Q000097:blood; D006454:Hemoglobins / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008722:Methods; D010101:Oxygen Consumption
D006454:Hemoglobins; D002245:Carbon Dioxide
Méthode d'enregistrement en continu sur micro-échantillons de la courbe d'association Hb-O2. II. Etude de l'effet Bohr et de la carbamino-formation