Cholesteryl ester and triglyceride hydrolysis by an acid lipase from rabbit aorta.
The properties of the triglyceride- and cholesteryl ester-hydrolyzing activity by an acid lipase from rabbit aortic tissue were compared under different experimental conditions. Radiolabeled cholesteryl oleate or triolein was incorporated into phospholipid vesicles by sonication and the resulting preparations were used for in vitro studies. No distinction was observed between triglyceride lipase and cholesterol esterase activity in the aortic cytosol fraction following either thermal inactivation, inhibition by a mercurial, fractionation by ammonium sulfate or acid precipitation, or DEAE-cellulose chromatography. Addition of rabbit lipoproteins to the assay system resulted in inhibition of both cholesterol esterase and triglyceride lipase activity. Parallel changes in the hydrolysis of both substrates also were observed when exogenously added lipids were added to the incubation system in various physical states. Specificities of the enzyme system towards different cholesteryl esters were examined. No differences in the rate of hydrolysis were observed between cholesteryl oleate, palmitate and linoleate. The data suggest that a single acid lipase, presumably of lysosomal origin, has broad specificity towards triglycerides and cholesteryl esters, and may play a role in the hydrolysis of these lipids during intralysosomal degradation of lipoproteins.
D000818:Animals; D001011:Aorta / Q000201:enzymology*; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D002788:Cholesterol Esters / Q000378:metabolism*; D003600:Cytosol; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008049:Lipase / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D011817:Rabbits; D002787:Sterol Esterase / Q000302:isolation & purification / Q000378:metabolism*; D013379:Substrate Specificity; D014280:Triglycerides / Q000378:metabolism*
D002788:Cholesterol Esters; D014280:Triglycerides; D002265:Carboxylic Ester Hydrolases; D002787:Sterol Esterase; D008049:Lipase