An improved method for the determination of acetylcholinesterase activity in rectal biopsy tissue from patients with Hirschsprung's disease.
A method is described for the assay of acetylcholinesterase in tissue samples using the selective cholinesterase inhibitor, lysivane (10-(alpha-diethylaminopropyl) phenothiazine hydrochloride). Significantly increased enzyme activity is found in rectal biopsy specimens from patients with Hirschsprung's disease (p less than 0.001) indicating the diagnostic usefulness of the assay.
D000110:Acetylcholinesterase / Q000378:metabolism*; D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D002800:Cholinesterase Inhibitors; D002802:Cholinesterases / Q000378:metabolism; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008531:Megacolon / Q000201:enzymology*; D008722:Methods; D010640:Phenothiazines; D012007:Rectum / Q000201:enzymology*
D002800:Cholinesterase Inhibitors; D010640:Phenothiazines; D000110:Acetylcholinesterase; D002802:Cholinesterases