Interaction of apoA-II from human high density lipoprotein with lysolecithin.
The effects of lysolecithin and hexadecyltrimethylammonium bromide on the structure and stability of apoA-II from human high density lipoprotein have been evalued by circular dichroism and fluorescence measurements. There is a profound enhancement in the stability of apoA-II to guanidinium hydrochloride denaturation when it forms a phospholipid complex with lysolecithin micelles. This complex is not only resistant to guanidinium hydrochloride denaturation, but it can be formed in a 6 M solution of this denaturant. The behavior of apoA-II in the native human high density protein is much closer to that of the lysolecithin apoA-II complex than to that of the free apoA-II.
D001059:Apoproteins; D002593:Cetrimonium Compounds / Q000494:pharmacology; D002942:Circular Dichroism; D006146:Guanidines / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008075:Lipoproteins, HDL; D008244:Lysophosphatidylcholines / Q000494:pharmacology*; D011487:Protein Conformation / Q000187:drug effects; D011489:Protein Denaturation / Q000187:drug effects; D013050:Spectrometry, Fluorescence
D001059:Apoproteins; D002593:Cetrimonium Compounds; D006146:Guanidines; D008075:Lipoproteins, HDL; D008244:Lysophosphatidylcholines