Interactions of some acceptors with superoxide anion radicals formed by the NADPH-specific flavoprotein in rat liver microsomal fractions.
In rat liver microsomal fractions oxidation of adrenaline was effected by superoxide anion radicals (O2-), whereas cytochrome c, 2,6-dichlorophenol-indophenol and ferricyanide accepted electrons from NADPH-specific flavoprotein only directly. Nitro Blue Tetrazolium was reduced both by O2- and by the direct acceptance of electrons. Elevation of pH and addition of menadione shift the Nitro Blue Tetrazolium reduction towards the O2--dependent pathway. From the values of the kinetic constants for interaction of adrenaline and Nitro Blue Tetrazolium with NADPH-specific flavoprotein, the rates of generation of O2- in rat liver microsomal fraction were determined.
D015086:2,6-Dichloroindophenol / Q000378:metabolism; D000818:Animals; D003579:Cytochrome Reductases / Q000378:metabolism*; D003574:Cytochrome c Group / Q000378:metabolism; D004837:Epinephrine / Q000378:metabolism; D005292:Ferricyanides / Q000378:metabolism; D005420:Flavoproteins / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology / Q000378:metabolism*; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism*; D009580:Nitroblue Tetrazolium / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism*; D051381:Rats; D013482:Superoxide Dismutase; D013481:Superoxides / Q000378:metabolism*; D014812:Vitamin K
D003574:Cytochrome c Group; D005292:Ferricyanides; D005420:Flavoproteins; D013481:Superoxides; D014812:Vitamin K; D009580:Nitroblue Tetrazolium; D015086:2,6-Dichloroindophenol; D010088:Oxidoreductases; D013482:Superoxide Dismutase; D003579:Cytochrome Reductases; D009251:NADPH-Ferrihemoprotein Reductase; D010100:Oxygen; D004837:Epinephrine