[Erythrocyte stroma included in polyacrylamide gel. Applications to affinity chromatography].
Stromata prepared with human or animal red blood cells are suspended in acrylamide solution. Gel as prepared for electrophoresis is dispersed, before use, and can be used in chromatographic columns for the retention of agglutinins. Lectins, e.g., where first absorbed and then eluted with solution of inhibitory sugar or with acid buffer. Some applications are mentioned.
D000178:Acrylamides; D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D001665:Binding Sites; D002846:Chromatography, Affinity / Q000379:methods*; D004912:Erythrocytes / Q000276:immunology; D006388:Hemagglutinins / Q000302:isolation & purification; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000302:isolation & purification; D007075:Immunoglobulin M / Q000302:isolation & purification; D037102:Lectins / Q000302:isolation & purification
D000178:Acrylamides; D000906:Antibodies; D006388:Hemagglutinins; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D037102:Lectins
Les stromas érythrocytaires inclus en gel de polyacrylamide. Applications à la chromatographie d'affinité