Acceleration of autoimmunity in NZB/NZW F1 mice by graft-versus-host disease.
Chronic graft-versus-host (GVH) disease was induced in NZB/NZW F1 (B/W) hybrid female mice by the weekly injection of parental NZB spleen cells. Control mice received injections of syngeneic spleen cells only. The mice were assayed for antibodies to [3H]DNA and [3H]polyadenylic-polyuridylic acid by a cellulose ester filter radioimmunoassay, and for antibody to thymocytes by a cytotoxicity method. GVH disease accelerated the development of all three antibodies in B/W mice. In addition, sucrose density gradient ultracentrifugation of pooled sera suggested that an accelerated switch from 19S to 7S anti-DNA production may be an early effect of GVH. The mechanism of acceleration is discussed in terms of immunological and viral factors generated by the GVH reaction.
D000818:Animals; D001323:Autoantibodies; D003601:Cytotoxicity Tests, Immunologic; D004247:DNA / Q000276:immunology; D005260:Female; D006087:Graft vs Host Reaction; D051379:Mice; D008814:Mice, Inbred NZB / Q000276:immunology*; D011063:Poly A-U / Q000276:immunology; D012313:RNA / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D001323:Autoantibodies; D011063:Poly A-U; D012313:RNA; D004247:DNA