[Activity of NAD- and NADP-dependent malate dehydrogenase isoenzymes in the myocardium of rabbits with alloxan diabetes].
Isoenzymes NAD-and NaDP MDH were detected in the cardiac muscle of rabbits by disc electrophoresis in polyacrylamide gel. Alloxan diabetes proved to be accompanied by a significant reduction in the activity of mitochondrial NADP MDH (in the reaction of malic decarboxylation) and its increase in cytozol. The activity of NAD-MDH (in the reaction of oxyacetate reduction) was also decreased in various isoenzymes in the myocardium (particularly in the mitochondria) in diabetes. Insulin restored the correlation of the activities of the isoenzymes NAD- and NADP-MDH in the cytostructures of the myocardium disturbed in diabetes.
D000818:Animals; D003600:Cytosol / Q000201:enzymology; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology*; D007527:Isoenzymes / Q000378:metabolism*; D008291:Malate Dehydrogenase / Q000378:metabolism*; D008931:Mitochondria, Muscle / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology*; D009243:NAD; D009249:NADP; D011817:Rabbits
D007527:Isoenzymes; D009243:NAD; D009249:NADP; D008291:Malate Dehydrogenase
Aktivnost' izofermentov NAD- i NADF-zavisimykh malatdegidrogenaz v miokarde krolikov s alloksanovym diabetom