[Enzyme-immunological tests: determination of the activity of peroxidase with the aid of the "Trinder reagent" (author's transl)].
Most of the currently used enzyme-immunological tests employ horse radish peroxidase as a marker enzyme. A new method is described for the determination of extremely small quantities of peroxidase. This largely prevents the inactivation of the peroxidase by H2O2 and thereby permits a much longer incubation time. In the presence of 25 mmol/l phenol, 2 mmol/l 4-amino-antipyrin and 0.8 mmol/l H2O2, peroxidase catalyses the formation of a red quinonimine, whose increase in adsorption is directly proportional to the enzyme concentration.
D000632:Aminopyrine; D001798:Blood Proteins; D006735:Horseradish Peroxidase / Q000032:analysis; D006861:Hydrogen Peroxide; D006863:Hydrogen-Ion Concentration; D007124:Immunoenzyme Techniques; D007202:Indicators and Reagents; D007700:Kinetics; D008832:Microchemistry; D010636:Phenols; D013053:Spectrophotometry / Q000379:methods; D013696:Temperature
D001798:Blood Proteins; D007202:Indicators and Reagents; D010636:Phenols; D000632:Aminopyrine; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase
Enzym-immunologische Tests: Aktivitätsbestimmung von Peroxidase mit Hilfe des "Trinder-Reagens".