The effects of pH and temperature on the assay of superoxide dismutase.
A simple and reliable method for the measurement of superoxide dismutase (EC 1.15.1.1) activity is described. The method is based on a linear inhibition of the reduction of acetylated cytochrome c by superoxide dismutase.
D001395:Azotobacter / Q000201:enzymology*; D003574:Cytochrome c Group / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction; D013482:Superoxide Dismutase / Q000378:metabolism*; D013696:Temperature; D014969:Xanthine Oxidase / Q000378:metabolism
D003574:Cytochrome c Group; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase