Suitability of some enrichment broths and diluents for enumerating cold- and heat-stressed Vibrio parahaemolyticus.
Vibrio parahaemolyticus cells were injured by chilling and heating, and their recovery was  tested in glucose-salt-Teepol broth (GSTB), tryptic soy broth containing 7% NaCl (TSBS), Horie - arabinose - ethyl violet broth (HAEB), and water blue - alizarin yellow broth (WBAY). Exponential phase cells were more sensitive to cold shock than were stationary phase cells. Exposure of chill-injured V. parahaemolyticus to GSTB and TSBS resulted in 70 to 80% death; about 70% lethality was noted for heat-injured cells inoculated into TSBS. Neither HAEB nor WBAY enrichment media were lethal to stressed cells, although rates of growth were retarded. The 3% NaCl in 0.1 M potassium phosphate (pH 7.0) diluent proved to be most suitable for  protecting against inactivation of cold- and heat-injured cells.
D003080:Cold Temperature; D003470:Culture Media; D005069:Evaluation Studies as Topic; D005516:Food Microbiology; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D010710:Phosphates; D012965:Sodium Chloride; D014736:Vibrio parahaemolyticus / Q000254:growth & development / Q000302:isolation & purification*
D003470:Culture Media; D010710:Phosphates; D012965:Sodium Chloride