[The electrophoretic pattern of gamma-glutamyl transferase in serum and its alteration by chylomicrons (author's transl)].
Using the sera from 20 patients with elevated gamma-glutamyl transferase activity (EC 2.3.2.2) due to intra- or posthepatic cholestasis, the enzyme was separated into four bands by cellulose acetate foil electrophoresis. The gamma-glutamyl transferase pattern permitted no differentiation between intra- or posthepatic cholestasis. Protein and lipid electrophoresis was performed simultaneously for each serum. It was found that a gamma-glutamyl transferase band, which appeared at the origin in 11 sera, was only observed in lipaemic sera containing chylomicrons. This band of gamma-glutamyl transferase does not, however, represent a true isoenzyme, because it results from a complex between chylomicrons and gamma-glutamyl transferase, which is separated with the chylomicrons, or is produced by the mixing of sera.
D001652:Bile Ducts / Q000201:enzymology; D002779:Cholestasis / Q000201:enzymology; D002914:Chylomicrons / Q000097:blood*; D004588:Electrophoresis, Cellulose Acetate; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood; D007527:Isoenzymes / Q000097:blood; D008099:Liver / Q000201:enzymology; D005723:gamma-Glutamyltransferase / Q000097:blood*
D002914:Chylomicrons; D007527:Isoenzymes; D005723:gamma-Glutamyltransferase
Das elektrophoretische Muster der gamma-Glutamyltransferase im Serum und seine Anderung durch Chylomikronen