Ecdysome 20-hydroxylase from the midgut of the tobacco hornworm (Manduca sexta L.).
An ecdysone 20-hydroxylase enzyme system that converts alpha-ecdysone to 20-hydroxyecdysone was prepared from the midgut of the tobacco hornworm prepupa. This partially purified enzyme is NADPH dependent and is localized in the mitochondrial fraction of the midgut tissue.
D000375:Aging; D000818:Animals; D004440:Ecdysone / Q000378:metabolism*; D005260:Female; D007422:Intestines / Q000201:enzymology*; D007814:Larva; D007915:Lepidoptera / Q000201:enzymology*; D008297:Male; D008928:Mitochondria / Q000201:enzymology; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D009036:Moths / Q000201:enzymology*; D009249:NADP / Q000378:metabolism; D011679:Pupa
D004440:Ecdysone; D009249:NADP; D006899:Mixed Function Oxygenases