pH dependence of the oxidation-reduction potential of cytochrome c2.
The pH dependence of the spectra and of the oxidation-reduction potential of three cytochromes c2, from Rhodopseudomonas capsulata, Rhodopseudomonas sphaeroides and Rhodomicrobium vannielii, were studied. A single alkaline pK was observed for the spectral changes in all three ferricytochromes. In Rps. capsulata cytochrome c2 this spectroscopic pK corresponds to the pK observed in the dependence of oxidation-reduction potential on pH. For the other two cytochromes the oxidation-reduction potential showed a complex dependency on pH which can be fitted to theoretical curves involving three ionizations. The third ionization corresponds to the ionization observed in the spectroscopic studies but the first two occur without changes in the visible spectra. The possible structural bases for these ionizations are discussed.
D003574:Cytochrome c Group / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008433:Mathematics; D008928:Mitochondria / Q000201:enzymology; D010084:Oxidation-Reduction; D011199:Potentiometry; D012242:Rhodobacter sphaeroides / Q000201:enzymology; D012241:Rhodopseudomonas / Q000201:enzymology; D012244:Rhodospirillaceae / Q000201:enzymology; D013045:Species Specificity
D003574:Cytochrome c Group