New trends in cryoenzymology: I.-Supercooled aqueous solutions.
A water in oil emulsion technique is proposed to investigate enzyme catalyzed reactions at sub-zero temperatures in the supercooled liquid state to avoid some reversible effects of the usual cosolvents on kinetics. Some results are listed: potentialities and technical problems of the procedure are discussed.
D000421:Alcaligenes / Q000201:enzymology; D002021:Buffers; D002248:Carbon Monoxide / Q000378:metabolism; D004655:Emulsions; D005486:Flavin Mononucleotide / Q000378:metabolism; D005615:Freezing; D006454:Hemoglobins / Q000378:metabolism; D006735:Horseradish Peroxidase / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008156:Luciferases / Q000378:metabolism*; D010544:Peroxidases / Q000378:metabolism*; D010782:Photolysis; D013696:Temperature
D002021:Buffers; D004655:Emulsions; D006454:Hemoglobins; D005486:Flavin Mononucleotide; D002248:Carbon Monoxide; D006735:Horseradish Peroxidase; D010544:Peroxidases; D008156:Luciferases