Alanine dehydrogenase of the N2-fixing blue-green alga, Anabaena cylindrica.
The L-alanine dehydrogenase (ADH) of Anabaena cylindrica has been purified 700-fold. It has a molecular weight of approximately 270,000, has 6 sub-units, each of molecular weight approximately 43,000, and shows activity both in the aminating and deaminating directions. The enzyme is NADH/NAD+ specific and oxaloacetate can partially substitute for pyruvate. The Kampp for NAD+ is 14 muM and 60 muM at low and high NAD concentrations respectively.
D000409:Alanine / Q000378:metabolism; D000594:Amino Acid Oxidoreductases / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D000643:Ammonium Chloride / Q000378:metabolism; D000458:Cyanobacteria / Q000201:enzymology*; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008970:Molecular Weight; D009243:NAD / Q000378:metabolism; D009586:Nitrogen Fixation; D011773:Pyruvates / Q000378:metabolism
D011773:Pyruvates; D000643:Ammonium Chloride; D009243:NAD; D000594:Amino Acid Oxidoreductases; D005969:Glutamate Dehydrogenase; D005974:Glutamate-Ammonia Ligase; D000409:Alanine