Manganese mutagenesis in yeast. A practical application of manganese for the induction of mitochondrial antibiotic-resistant mutations.
When yeast cells were incubated for 4 to 8 h in yeast extract-peptone-glucose medium, pH 6, containing 8 mM-manganese, and then plated on selective media, there was a strong induction of antibiotic-resistant mutations. Indirect evidence suggests that practically all resistant mutants selected were of independent origin. The analysis of manganese-induced resistant mutants showed that most were extranuclear, while those tested showed recombination with known mitochondrial markers. Our results suggest that manganese can be considered as a mutagen which specifically induces mitochondrial mutations in Saccharomyces cerevisiae.
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002455:Cell Division; D004352:Drug Resistance, Microbial; D005111:Extrachromosomal Inheritance; D006863:Hydrogen-Ion Concentration; D008345:Manganese / Q000494:pharmacology*; D008928:Mitochondria / Q000187:drug effects; D009153:Mutagens; D009154:Mutation; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000254:growth & development
D000900:Anti-Bacterial Agents; D009153:Mutagens; D008345:Manganese