Alpha-l-arabinofuranosidase from Rhodotorula flava.
An alpha-L-arabinofuranosidase (EC 3.2.1.55) from the culture fluid of Rhodotorula flava IFO 0407 grown on beet arabinan as a carbon source has been highly purified. The purified enzyme has a pH optimum of 2.0. The enzyme is unusually acid stable, retaining 82% of its activity after being maintained for 24 h at pH 1.5 and at 30 degrees C. The apparent Km and Vmax values of the enzyme for phenyl alpha-L-arabinofuranoside were determined to be 9.1 mM and 72.5 mumol per min per mg of protein, respectively.
D001089:Arabinose; D003300:Copper / Q000494:pharmacology; D006026:Glycoside Hydrolases / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008628:Mercury / Q000494:pharmacology; D003904:Mitosporic Fungi / Q000201:enzymology*; D012248:Rhodotorula / Q000201:enzymology*; D012834:Silver / Q000494:pharmacology; D014001:Tin / Q000494:pharmacology
D012834:Silver; D014001:Tin; D003300:Copper; D001089:Arabinose; D006026:Glycoside Hydrolases; C019713:alpha-N-arabinofuranosidase; D008628:Mercury