The biochemical pathway for the breakdown of N4-ethyl-L-asparagine in the bacterium Pseudomonas stutzeri.
N4-Ethyl-L-[u-14C]asparagine and L-[U-14C]aspartate give identical metabolites, mainly intermediates of the tricarboxylic acid cycle and related amino acids, in whole cells of Pseudomonas stutzeri. The labelled asparagine derivative is converted into [14C]-aspartate by cell-free extracts, and this reaction, which has an optimum pH of 8.8 +/- 0.2, is neither inhibited by unlabelled asparagine nor enhanced by unlabelled 2-oxoglutarate. No labelled keto acid corresponding to N4-ethylasparagine was detected in either whole cells or cell-free extracts. Thus N4-ethyl-L-asparagine, like asparagine, must be broken down by hydrolysis, at least in this bacterium.
D000596:Amino Acids / Q000378:metabolism; D001216:Asparagine / Q000031:analogs & derivatives* / Q000378:metabolism; D001224:Aspartic Acid / Q000378:metabolism; D002474:Cell-Free System / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007656:Ketoglutaric Acids / Q000378:metabolism; D011549:Pseudomonas / Q000378:metabolism*
D000596:Amino Acids; D007656:Ketoglutaric Acids; D001224:Aspartic Acid; D001216:Asparagine