[New variant of glucose-6-phosphate dehydrogenase (G-6-PD "Kaluga") from erythrocytes of a patient with chronic nonspherocytic hemolytic anemia].
Properties of a new form of glucose-6-phosphate dehydrogenase (G6PD) from erythrocytes, called "Kaluga", are described. The enzyme was isolated from erythrocytes of a patient with chronic non-spherocytic hemolytic anemia; in the blood cells 20% of the G6PD activity was maintained as compared with normal state. The partially purified enzyme was shown to be unstable in electrophoresis, it possessed a biphase type of pH-optima; Km value for NADP was decreased for glucose-6-phosphate Km value was normal. The thermostability of G6PD was normal at 46 within 1 hr.
D000746:Anemia, Hemolytic, Congenital Nonspherocytic / Q000097:blood / Q000201:enzymology*; D002848:Chromatography, DEAE-Cellulose; D002908:Chronic Disease; D004592:Electrophoresis, Starch Gel; D004912:Erythrocytes / Q000201:enzymology*; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis* / Q000302:isolation & purification; D005958:Glucosephosphates; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009249:NADP; D013053:Spectrophotometry
D005958:Glucosephosphates; D009249:NADP; D005954:Glucosephosphate Dehydrogenase
Opisanie novogo varianta gliukozo-6-fosfatdegidrogenazy eritrotsitov (G-6-FD "Kaluga"), soprovozhdaiushchegosia khronicheskoĭ nesferotsitarnoĭ gemoliticheskoĭ anemiei