The dissociation of insect embryos for cell culture.
Procedures and solutions were developed for dissociating embryos of Blattella germanica in preparation for primary cell culture. Trypsin solutions were maximally effective at 0.01% for germ bands but higher concentrations, 0.05 to 0.1% were needed for embryos in later stages.
D000818:Animals; D002469:Cell Separation; D003058:Cockroaches / Q000166:cytology* / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004492:Edetic Acid / Q000494:pharmacology; D004625:Embryo, Nonmammalian / Q000187:drug effects; D006801:Humans; D006863:Hydrogen-Ion Concentration; D014357:Trypsin / Q000494:pharmacology
D004492:Edetic Acid; D014357:Trypsin