SIF cells, cyclic AMP responses, and catecholamines of the guinea pig superior cervical ganglion.
The superior cervical ganglion (SCG) of the guinea pig has been investigated by a multidisciplinary approach. Dopamine (50 micron) produced no increase in cyclic AMP levels above control values of 27.9 pmole/mg protein, but 50 micron isoproterenol produced cyclic AMP levels of 210 pmole/mg protein, indicating the existence of a beta-adrenergic receptor-adenylate cyclase complex. The SIF cells were studied by fluorescence histochemistry, which indicated that two morphological types were present. A few Type I cells of the guinea pig SCG were solitary, but most were present in clusters containing many Type II cells. Immunohistochemical localization of antibodies to dopamine-beta-hydroxylase (DBH) and phenylethanolamine-N-methyltransferase (PNMT) demonstrated that types of SIF cell localize antibodies to DBH but not PNMT, providing strong evidence that norepinephrine is the neurotransmitter for all the SIF cells of the guinea pig SCG. Determination of the ratio of norepinephrine to dopamine confirmed that no other dopamine pools exist in the guinea pig SCG.
D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D002572:Cervical Plexus / Q000166:cytology* / Q000201:enzymology; D000242:Cyclic AMP / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism / Q000494:pharmacology; D004299:Dopamine beta-Hydroxylase / Q000378:metabolism; D006168:Guinea Pigs; D007545:Isoproterenol / Q000494:pharmacology; D018377:Neurotransmitter Agents / Q000494:pharmacology*; D009638:Norepinephrine / Q000378:metabolism / Q000494:pharmacology; D010625:Phenylethanolamine N-Methyltransferase / Q000378:metabolism; D011943:Receptors, Adrenergic, beta / Q000187:drug effects / Q000378:metabolism
D018377:Neurotransmitter Agents; D011943:Receptors, Adrenergic, beta; D000242:Cyclic AMP; D004299:Dopamine beta-Hydroxylase; D010625:Phenylethanolamine N-Methyltransferase; D000262:Adenylyl Cyclases; D007545:Isoproterenol; D004298:Dopamine; D009638:Norepinephrine