Formation of stable crystalline enzyme-substrate intermediates at sub-zero temperatures.
Sub-zero temperatures can be used to trap intermediates in enzyme-catalysed reactions using suitable cryosolvents. The feasibility of obtaining such intermediates in the crystalline state for X-ray diffraction studies has been demonstrated with several proteases, using specific substrates and optimal pH.
D002384:Catalysis; D002918:Chymotrypsin / Q000378:metabolism*; D003080:Cold Temperature; D003460:Crystallization; D004121:Dimethyl Sulfoxide; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D000432:Methanol; D009596:Nitrophenols / Q000378:metabolism; D010196:Pancreatic Elastase / Q000378:metabolism*; D014357:Trypsin / Q000378:metabolism*; D014961:X-Ray Diffraction
D009596:Nitrophenols; D002918:Chymotrypsin; D010196:Pancreatic Elastase; D014357:Trypsin; D000432:Methanol; D004121:Dimethyl Sulfoxide