[Indirect immunoprecipitation by rat liver polyribosomes using antibodies to tyrosine aminotransferase].
A fraction of rat liver polyribosomes is isolated, which in its immunochemical characteristics considerably enriched with polyribosomes capable to synthesize hydrocortisone-induced liver tyrosine aminotransferase isoenzyme. This specific polyribosome fraction was purified by immunochemical fractionation of total liver polyribosomes using indirect precipitation. The content of polyribosomes in immunoprecipitates comprise 0.4-0.8% of its initial amount (before immunochemical fractionation). The ratio of specific polyribosomes in immunoprecipitates varies from 20 to 45%, which corresponds to 25-100-fold purification. The data obtained suggest that the method of indirect precipitation can be an efficient step in the isolation procedure of individual mRNA.
D000818:Animals; D000906:Antibodies; D002458:Cell Fractionation / Q000379:methods; D007120:Immunochemistry / Q000379:methods; D008099:Liver / Q000648:ultrastructure*; D011132:Polyribosomes / Q000032:analysis*; D011233:Precipitin Tests; D012333:RNA, Messenger / Q000302:isolation & purification*; D051381:Rats; D014444:Tyrosine Transaminase / Q000096:biosynthesis* / Q000276:immunology
D000906:Antibodies; D012333:RNA, Messenger; D014444:Tyrosine Transaminase
Nepriamaia immunopretsipitatsiia poliribosom pecheni krys s pomoshch'iu antitel k tirozinaminotransferaze.