Effect of metabolites on epsilon-N-hydroxylysine formation in cell-free extracts of Aerobacter aerogenes 62-1.
The conversion of L-lysine to its corresponding epsilon-N-hydroxy derivative has been achieved for the first time by cell-free extracts of Aerobacter aerogenes 62-1. Partial fractionation by differential centrifugation (at 12 000 X g) revealed that both supernatant and pellet are essential for maximum enzymatic activity. The omega-N-hydroxylase (EC 1.14.99) was found to function optimally at pH 7-7.5 and exhibited an apparent Km of about 75 muM for L-lysine. L(+)-Lactate or DL-lactate and pyruvate greatly stimulate the omega-N-hydroxylase activity. The system is strongly inhibited by arsenite and sulfite.
D001151:Arsenic / Q000494:pharmacology; D002474:Cell-Free System; D004754:Enterobacter / Q000378:metabolism*; D004755:Enterobacteriaceae / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006901:Hydroxylysine / Q000096:biosynthesis*; D007773:Lactates / Q000494:pharmacology; D008239:Lysine / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D011773:Pyruvates / Q000494:pharmacology; D013329:Structure-Activity Relationship; D013347:Subcellular Fractions / Q000378:metabolism; D013447:Sulfites / Q000494:pharmacology
D007773:Lactates; D011773:Pyruvates; D013447:Sulfites; D006901:Hydroxylysine; D006899:Mixed Function Oxygenases; C021404:hydroxylysine omega-N-hydroxylase; D008239:Lysine; D001151:Arsenic