[Inhibition of mouse spleen inorganic pyrophosphatase by methylene diphosphonic acid].
Some properties of mouse spleen cytosol inorgainc pyrophosphatase (PPi-ase) (E. C. 3.6.1.1) as well as the effect of methylene diphosphonic acid (PCP) on the PPi-ase activity were studied. Specific staining for the enzyme PAAG disc-electrophoresis was developed; it was shown that the PPi-ase formed only one band in 7.5% PAAG. The enzyme pH optimum being 8.0, the optimal [Mg++]/[PPi] ratio was about 2; Km =7.7x10(-4) M, Vmax=0.77 mkM. min-1. mg protein-1. PCP was shown to competitively inhibit the pyrophosphatase reaction, Ki=2.5x10(-4) M +/- 0.2x10(-4) M.
D000818:Animals; D001667:Binding, Competitive; D003600:Cytosol / Q000201:enzymology; D004164:Diphosphonates / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000494:pharmacology; D051379:Mice; D011755:Pyrophosphatases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D013154:Spleen / Q000201:enzymology*
D004164:Diphosphonates; D011755:Pyrophosphatases; D008274:Magnesium
Ingibirovanie metilendifosfonovoĭ kislotoĭ aktivnosti neorganicheskoĭ pirofosfatazy selezenki mysheĭ