A method for enhancing the stability of thiol-containing enzymes immobilised on nylon.
The limited stability of immobilised enzymes is a major factor restricting their use in clinical chemistry. The immobilisation procedure described here affords a simple method of enhancing the stability of certain enzymes immobilised on nylon. Studies with alcohol dehydrogenase and glyceraldehyde-3-phosphate dehydrogenase have indicated that a significant increase in stability can be obtained using this immobilisation procedure as compared with conventional procedures. As anticipated the immobilisation procedure was unsatisfactory for oxidases such as glucose oxidase.
D000429:Alcohol Oxidoreductases / Q000378:metabolism; D004355:Drug Stability; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005949:Glucose Oxidase / Q000378:metabolism; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008722:Methods; D009757:Nylons; D013438:Sulfhydryl Compounds / Q000378:metabolism
D004800:Enzymes, Immobilized; D009757:Nylons; D013438:Sulfhydryl Compounds; D000429:Alcohol Oxidoreductases; D005949:Glucose Oxidase; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases