Improved histological localization of GABA-transaminase activity in rat cerebellar cortex after aldehyde fixation.
A method for the chemical fixation of the enzyme GABA-transaminase in nervous tissue is described. It is shown that after perfusion with a formaldehyde/glutaraldehyde fixative, activity of the enzyme in cerebellar cortex is demonstrable whilst cellular morphology is preserved. Results from the improved technique have shown new sites of GABA-transaminase activity in cerebellar cortex. In view of these results a special function for glial cells in this area of brain has been suggested.
D000612:4-Aminobutyrate Transaminase / Q000378:metabolism*; D000818:Animals; D002525:Cerebellar Cortex / Q000201:enzymology* / Q000502:physiology; D005260:Female; D005557:Formaldehyde; D005976:Glutaral; D006651:Histocytochemistry; D006652:Histological Techniques; D006863:Hydrogen-Ion Concentration; D008297:Male; D009433:Neural Inhibition; D011689:Purkinje Cells / Q000201:enzymology; D051381:Rats; D013569:Synapses / Q000201:enzymology; D013696:Temperature; D000637:Transaminases / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid
D005557:Formaldehyde; D005680:gamma-Aminobutyric Acid; D000637:Transaminases; D000612:4-Aminobutyrate Transaminase; D005976:Glutaral