[Assay of aminoglycosides in serum using the urease method (author's transl)].
The urease method developed by Noone et al. for rapid bioassay of aminoglycoside antibiotics in serum is described in detail. The accuracy of the method was improved by using a BM 1-09 electrode assembly (Metrohm, CH-9100 Herisau, Switzerland) in conjunction with a digital pH-Meter (Metrohm E 500). The mean difference between spiked serum samples and measured concentrations was +0.37 +/-0.78 microgram/ml. The coefficient of determination between the urease and agar diffusion method was 0.94. Disadvantages of the method are a low sensitivity at concentrations below 1.5 microgram/ml and a large serum sample (1.5 ml). Advantages are its simplicity and rapidity.
D000617:Aminoglycosides / Q000097:blood*; D005069:Evaluation Studies as Topic; D005839:Gentamicins / Q000097:blood*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008722:Methods; D014510:Urease
D000617:Aminoglycosides; D005839:Gentamicins; D014510:Urease
Aminoglykosid-Bestimmungen im Serum mit der Urease-Methode.