Infection of Hela cells with a virulent and an attenuated strain of pseudorabies virus studied by electron microscopy.
Hollow-core particles, forming crystals in nuclei, prevailed in HeLa cells infected with an attenuated strain of pseudorabies virus (PRV). After infection with a virulent PRV strain, the cells contained mainly fully infectious dense-core particles. These findings might explain the lower susceptibility of HeLa and some other human cells to infection with attenuated strains of PRV as compared to virulent strains.
D002467:Cell Nucleus / Q000382:microbiology; D003461:Crystallography; D003588:Cytopathogenic Effect, Viral; D003593:Cytoplasm / Q000382:microbiology; D006367:HeLa Cells; D006564:Herpesviridae / Q000254:growth & development*; D011558:Herpesvirus 1, Suid / Q000254:growth & development* / Q000472:pathogenicity / Q000648:ultrastructure; D007181:Inclusion Bodies, Viral; D008854:Microscopy, Electron; D014774:Virulence; D014779:Virus Replication