A radioimmunoassay for 1-beta-D-arabinofuranosyluracil with reference to cross-reactivity of 1-beta-D-arabinofuranosylcytosine with an antibody.
Antibodies directed against 1-beta-D-arabinofuranosyluracil have been produced in rabbits by immunization with a conjugate of 1-(5-O-succinyl-beta-D-arabinofuranosyl)uracil with human serum albumin. Two of four antibodies so obtained showed high specificity for 1-beta-D-arabinofuranosyluracil and allowed the development of a sensitive and reliable radioimmunoassay for this substrate. On the other hand, one antibody had a high affinity for 1-beta-D-arabinofuranosylcytosine. The binding of 1-beta-D-arabinofuranosylcytosine to this antibody was practically constant between pH 5.2 and 9.0, whereas 1-beta-D-arabinofuranosyluracil binding was affected drastically by pH. The pH-binding profile for 1-beta-D-arabinofuranosylcytosine and 1-beta-D-arabinofuranosyluracil was reminiscent of the specificity of ara-C-specific antibodies, which we previously obtained after immunization of rabbits with 1-(5-O-succinyl-beta-D-arabinofuranosyl)cytosine as a hapten.
D000906:Antibodies; D000918:Antibody Specificity; D001086:Arabinofuranosyluracil / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D001666:Binding Sites, Antibody; D003429:Cross Reactions; D003561:Cytarabine / Q000031:analogs & derivatives / Q000276:immunology* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D011741:Pyrimidine Nucleosides / Q000032:analysis*; D011863:Radioimmunoassay
D000906:Antibodies; D011741:Pyrimidine Nucleosides; D003561:Cytarabine; D001086:Arabinofuranosyluracil