Defective synthesis of lipid intermediates for peptidoglycan formation in a stabilized L-form of Streptococcus pyogenes.
Membrane preparations obtained from a stabilized L-form of Streptococcus pyogenes are incapable of synthesizing peptidoglycan from uridine-5'-diphospho-N-acetyl-D-muramyl-L-Ala-D-iso-Glu-L-Lys-D-Ala-D-Ala and uridine-5'-diphospho-N-acetyl-D-glucosamine, in contrast with similar preparations from the parental streptococcus. Furthermore, 50-fold higher levels of lipid intermediates which serve as membrane-bound substrates for peptidoglycan synthesis are synthesized in reaction mixtures containing streptococcal membranes than with similar preparations from the L-form. These observations suggest that the inability of this stabilized L-form to form a cell wall in vivo lies, at least in part, in its failure to synthesize significant quantities of the lipid substrates for peptidoglycan synthesis.
D000255:Adenosine Triphosphate / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D002474:Cell-Free System; D006863:Hydrogen-Ion Concentration; D007740:L Forms / Q000378:metabolism*; D008025:Ligases / Q000378:metabolism; D008055:Lipids / Q000096:biosynthesis*; D010457:Peptidoglycan / Q000096:biosynthesis*; D010770:Phosphotransferases / Q000378:metabolism; D013297:Streptococcus pyogenes / Q000378:metabolism*; D013696:Temperature; D014500:Uracil Nucleotides / Q000378:metabolism; D014537:Uridine Diphosphate N-Acetylglucosamine / Q000378:metabolism
D008055:Lipids; D010457:Peptidoglycan; D014500:Uracil Nucleotides; D014537:Uridine Diphosphate N-Acetylglucosamine; D000255:Adenosine Triphosphate; D010770:Phosphotransferases; D008025:Ligases