Inorganic nitrogen assimilation by the photosynthetic bacterium Rhodopseudomonas capsulata.
The photosynthetic bacterium Rhodopseudomonas capsulata lacks glutamate dehydrogenase and normally uses the glutamine synthetase/glutamate synthase sequence of reactions for assimilation of N2 and ammonia. The glutamine synthetase in cell-free extracts of the organism is completely sedimented by centrifugation at 140,000 X g for 2 h, is inhibited by L-alanine but not by adenosine 5'-monophosphate, and exhibits two apparent Km values for ammonia (ca. 13 muM and 1 mM).
D000249:Adenosine Monophosphate / Q000494:pharmacology; D000409:Alanine / Q000494:pharmacology; D000594:Amino Acid Oxidoreductases / Q000378:metabolism; D000641:Ammonia / Q000378:metabolism*; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D005970:Glutamate Synthase / Q000378:metabolism; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism; D007700:Kinetics; D008717:Methionine Sulfoximine / Q000494:pharmacology; D009584:Nitrogen / Q000378:metabolism*; D012241:Rhodopseudomonas / Q000201:enzymology / Q000378:metabolism*
D008717:Methionine Sulfoximine; D000249:Adenosine Monophosphate; D000641:Ammonia; D000594:Amino Acid Oxidoreductases; D005970:Glutamate Synthase; D005969:Glutamate Dehydrogenase; D005974:Glutamate-Ammonia Ligase; D009584:Nitrogen; D000409:Alanine