Electrophoretic demonstration and inital characterization of human red cell NAD(P)+ase.
A method for electrophoretic detection of NAD(P)+ase solubilized from red cell membranes is described. The method reveals both NAD+ase and NADP+ase and can be applied to a screening procedure for the detection of electrophoretic variants. The initial chracterization of the solubilized enzyme is also described.
D004588:Electrophoresis, Cellulose Acetate; D004910:Erythrocyte Membrane / Q000201:enzymology*; D004912:Erythrocytes / Q000201:enzymology*; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008722:Methods; D009699:N-Glycosyl Hydrolases / Q000097:blood* / Q000302:isolation & purification
D009699:N-Glycosyl Hydrolases