Glucose-6-phosphate dehydrogenase in vitro correlated with in vivo activity and reticulocytosis.
In vitro activity of erythrocyte glucose-6-phosphate dehydrogenase (G-6-PD) does not always correlate with in vivo hemolytic manifestations. Many of these non-correlations are reviewed and can now be explained on the basis of altered substrate affinity and/or altered inhibition by intracellular metabolites. These alterations are not detected by standard assay conditions. The influence of a young erythrocyte population upon in vitro G-6-PD activity was determined and the lower limit of expected values shown to be raised at least to the mean of an average age erythrocyte population.
D004905:Erythrocyte Aging; D004912:Erythrocytes / Q000201:enzymology*; D005954:Glucosephosphate Dehydrogenase / Q000097:blood* / Q000378:metabolism; D005955:Glucosephosphate Dehydrogenase Deficiency / Q000097:blood; D006461:Hemolysis; D006801:Humans; D066298:In Vitro Techniques; D012157:Mononuclear Phagocyte System / Q000201:enzymology; D009249:NADP / Q000097:blood
D009249:NADP; D005954:Glucosephosphate Dehydrogenase