[Studies on the properties of acid erythrocyte phosphatase in sheep and the isoenzymes of sheep and goat acid erythrocyte phosphatase].
Ovine erythrocytic acid phosphatase showed two peaks of activity at pH 5.0 and 5.7 in acetate buffer with p-nitrophenylphosphate as substrate. The enzyme was only slightly inhibited by fluoride and L-phenylalanine, but high concentrations of urea strongly inhibited it. Activity of the enzyme was greater in goat erythrocytes than in sheep. By means of starch electrophoresis, three isoenzymes belonging to nine types were separated from the ovine enzymes, while three isoenzymes of five types were present in goats. Electrophoresis in polyacrylamide gel was suitable for detecting the rapidly migrating isoenzymes.
D000135:Acid Phosphatase / Q000097:blood; D000818:Animals; D004791:Enzyme Inhibitors; D004912:Erythrocytes / Q000201:enzymology*; D005459:Fluorides / Q000494:pharmacology; D006041:Goats; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000378:metabolism; D008274:Magnesium / Q000494:pharmacology; D010635:Phenolphthaleins / Q000378:metabolism; D010649:Phenylalanine / Q000494:pharmacology; D012756:Sheep
D004791:Enzyme Inhibitors; D007527:Isoenzymes; D010635:Phenolphthaleins; D010649:Phenylalanine; D000135:Acid Phosphatase; D008274:Magnesium; D005459:Fluorides
Untersuchungen über die Eigenschaften der sauren Erythrozytenphosphatase vom Schaf und über die Isozyme der sauren Erythrozytenphosphatase bei Schafen und Ziegen