Splenic foci of cytotoxic lymphocytes in a graft-versus-host reaction.
It has been established that cytotoxic lymphocytes are generated in discrete foci in the spleens of mice undergoing a graft-versus-host reaction. The foci segregated randomly in such spleens. 2.8 X 10(4) lymph node cells, 2.3 X 10(5) spleen cells and 2.7 X 10(6) thymus cells from CBA mice generated a mean of one focus in lethally irradiated (CBA X DBA)F1 recipient mice. Cytotoxic foci were detected when cytotoxicity was measured by two different cytotoxicity assays, the release of [51Cr]-sodium chromate from [51Cr]-labelled target cells and colony inhibition. The seeding efficiency of cytotoxic focus-forming cells has been estimated at 19%.
D000818:Animals; D002478:Cells, Cultured; D002860:Chromium Radioisotopes; D003601:Cytotoxicity Tests, Immunologic; D004306:Dose-Response Relationship, Immunologic; D006087:Graft vs Host Reaction; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D013154:Spleen / Q000276:immunology*
D002860:Chromium Radioisotopes