[Effect of cocaine on the tyrosine hydroxylase of rat hypothalamus].
The influence of cocaine on tyrosine hydroxilase of rat brain hypothalamus was investigated in vivo (0.5 mg/kg) and in vitro (10(--6)--10(--5)M). Cocaine was used as a substance with a known adrenergic type of action. It was shown that under standard conditions cocaine in vitro increased the enzyme activity and decreased the Km for DMPH4 cofactor without changing Vmax of the reaction analyzed by the membrane enzyme. Cocaine in vitro decreased the tyrosine hydroxylase activity, especially that of the membrane enzyme. In this case there occurred a decrease of Km for DMPH4 and a decrease of Vmax of the reaction. The decrease of Vmax is considered to be the result of the secondary effect of cocaine.
D000818:Animals; D003042:Cocaine / Q000494:pharmacology*; D007031:Hypothalamus / Q000201:enzymology*; D008297:Male; D008566:Membranes / Q000201:enzymology; D051381:Rats; D012995:Solubility; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism*
D014446:Tyrosine 3-Monooxygenase; D003042:Cocaine
Vliianie kokaina na tirozingidroksilazu gipotalamusa krys