Endogenous activating factor for guanylate cyclase in synaptosomal-soluble fraction of rat brain.
When the crude mitochondrial fraction of rat brain was homogenized with distilled water and centrifuged, most of guanylate cyclase activity was detected in the soluble fraction. The total guanylate cyclase activity recovered in the soluble fraction was 5- to 8-fold higher than that of the crude mitochondrial fraction. The greater recovery of guanylate cyclase activity was found to be due to a release of an endogenous activating factor for guanylate cyclase. The activating factor was partially purified by acid extraction followed by a gel filtration and ion exchange resin columns. The factor was a dialyzable small molecule. The molecular weight was estimated to be between 300 and 600 by a Sephadex G-15 column and Diaflo ultrafilter membranes. It was stable in dilute acids, but labile in alkaline solution. It was readily soluble in water, but insoluble in organic solvents. Treatment with various enzymes, so far as tested, failed to abolish the activity. The activating factor stimulated the initial velocity of the reaction. It altered neither the Km value for GTP nor the dependency of the enzyme on divalent metals. The activation by the factor was due to an increase in the Vmax of the reaction. The activation was prevented by lysolecithin, Lubrol PX, hydroxylamine, methylhydroxylamine, or hemoglobin.
D000818:Animals; D001921:Brain / Q000201:enzymology*; D004789:Enzyme Activation; D005260:Female; D006162:Guanylate Cyclase / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D051381:Rats; D013347:Subcellular Fractions / Q000201:enzymology; D013574:Synaptosomes / Q000502:physiology*
D006162:Guanylate Cyclase