[Kinetics of N-dealkylation of amines with participation of microsomal cytochrome P-450].
Within the temperature range of 20-37 degrees C the kinetics of the demethylation reactions of a variety of amines with participation of hepatic microsomal cytochrome P-450, NADPH and O2 has been studied. Catalytical rate constants for all the substrates have been determined in generalized forms. Activation parameters deltaH* and deltaS* are also estimated. There is a linear relationship between deltaH* and deltaS*: deltaH*=20.7 kcal+366 degrees K deltaS*. Compensation relationship is characterized by a coefficient alpha=366 degrees/Taverage=1.21. The nature of the limiting step of the enzymatic amine demethylation involving cytochrome P-450 of hepatic microsomes is discussed.
D000588:Amines / Q000378:metabolism*; D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D003640:Dealkylation; D007700:Kinetics; D008862:Microsomes, Liver / Q000378:metabolism*; D009249:NADP / Q000378:metabolism; D010100:Oxygen; D051381:Rats; D013816:Thermodynamics
D000588:Amines; D009249:NADP; D003577:Cytochrome P-450 Enzyme System; D010100:Oxygen
Kinetika N-dealkilirovaniia aminov s uchastnem tsitokhroma P-450 mikrosom pecheni.