Chromogenic substrate assay of plasma prekallikrein. With a note on its site of biosynthesis.
A method for assaying plasma prekallikrein has been developed applying the chromogenic substrate Chromozym PK. Different variables have been investigated, and the final assay system was found to give a reproducible and reliable assay procedure. A normal value of 99.0 +/- 18% was found, the coefficient of variation being 7.9. Studies on material from patients with various liver diseases indicated that the main site of prekallikrein biosynthesis is the liver.
D000328:Adult; D005175:Factor XII Deficiency / Q000201:enzymology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007610:Kallikreins / Q000032:analysis* / Q000096:biosynthesis*; D008107:Liver Diseases / Q000201:enzymology; D008875:Middle Aged; D009994:Osmolar Concentration; D011288:Prekallikrein / Q000032:analysis* / Q000096:biosynthesis*; D013696:Temperature
D011288:Prekallikrein; D007610:Kallikreins