[Extraction of LHRH in human urine: study of the extraction of labelled synthetic hormone].
A method of extraction of synthetic LHRH is studied in human urines, using porous glass (Spherosil) and methanol. In the defined conditions the yield is greeter than 80%. It appears that the method is reproducible. The recovery varies essentially with the quantity of Spherosil and the pH of methanal. The use of methanol acidified at pH 3 increases the speed and the importance of labelled LHRH recovery.
D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000302:isolation & purification / Q000652:urine*; D006728:Hormones / Q000652:urine; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007457:Iodine Radioisotopes; D000432:Methanol
D006728:Hormones; D007457:Iodine Radioisotopes; D007987:Gonadotropin-Releasing Hormone; D000432:Methanol
Mise au point de l'extraction du LHRH dans les urines humaines: étude de l'extraction de l'hormone synthétique marquée