Isolation of streptococcal nuclease B by batch adsorption.
A method has been developed for the preparation of streptococcal nuclease B by batch adsorpton to diethylaminoethyl-cellulose. The enzyme is homogeneous with respect to nuclease activity and is suitable for use as an antigen in measurement of anti-deoxyribonuclease B levels in sera.
D000327:Adsorption; D000907:Antibodies, Bacterial / Q000032:analysis; D000942:Antigens, Bacterial; D003636:DEAE-Cellulose; D003851:Deoxyribonucleases / Q000276:immunology / Q000302:isolation & purification*; D003937:Diagnosis, Differential; D006801:Humans; D013290:Streptococcal Infections / Q000175:diagnosis; D013297:Streptococcus pyogenes / Q000201:enzymology* / Q000276:immunology
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D003636:DEAE-Cellulose; D003851:Deoxyribonucleases