Impaired renal H+ secretion and NH3 production in mineralocorticoid-deficient glucocorticoid-replete dogs.
When the administration of exogenous mineralocorticoid hormones was discontinued in adrenalectomized dogs maintained on glucocorticoid, net acid excretion decreased due largely to a reduction in urinary ammonium excretion (UNH4+V), and hyperchloremic hyperkalemic metabolic acidosis occurred and persisted. The reduction in UNH4+V was not associated with an increase in urine pH (UpH) or a decrease in urine flow, but correlated with the severity of hyperkalemia and was mitigated by dietary potassium restriction. UpH decreased to values as low as 5.3. During acidosis, UpH varied directly with UNH4+V, but in relation to UNH4+V, UpH exceeded that in acid-fed mineralocorticoid-replete dogs. Extrapolated to UNH4+V=0, however, UpH was not significantly different in the two groups (5.27 vs. 5.44). When distal delivery of sodium was increased by infusion of sodium phosphate, titratable acid excretion increased in both groups but pateaued at lower rates in the mineralocorticoid-deficient dogs. These results suggest that in mineralocorticoid-deficient dogs, renal ammonia production is diminished, in part due to potassium retention and hyperkalemia; renal hydrogen ion secretory capacity is reduced even when sodium and buffer delivery to the distal nephron is not reduced; and the ability of the kidney to generate normally steep urine-to-blood hydrogen ion concentration gradients is unimpaired.
D000138:Acidosis / Q000378:metabolism; D000450:Aldosterone / Q000502:physiology; D000641:Ammonia / Q000378:metabolism*; D000818:Animals; D001639:Bicarbonates / Q000378:metabolism; D001692:Biological Transport; D001769:Blood; D004285:Dogs; D005260:Female; D005938:Glucocorticoids / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006947:Hyperkalemia / Q000378:metabolism; D007477:Ions; D007668:Kidney / Q000378:metabolism*; D008901:Mineralocorticoids / Q000172:deficiency*; D009399:Nephrons / Q000502:physiology; D010710:Phosphates / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism; D014556:Urine
D001639:Bicarbonates; D005938:Glucocorticoids; D007477:Ions; D008901:Mineralocorticoids; D010710:Phosphates; D000450:Aldosterone; D000641:Ammonia; D006859:Hydrogen; D011188:Potassium