Improved methods of influenza virus propagation. II. Characteristics of cell culture and allantoic virus preparations.
Regular reproduction of influenza A virus with infectious titres of 7.4--8.5 log EID50/0.2 ml was observed in roller bovine embryo kidney cell cultures. The haemagglutinating activity of the cell culture preparations was 8-33-fold lower than that of the allantoic preparations, the infectivity being similar. Cell culture preparations of influenza A/Victoria/35/72 and A /Leningrad/538/74 viruses were markedly immunogenic in laboratory animals and antigenically active in complement fixation (CF) test. The CF activity of liquid and lyophilized preparations remained unchanged for 12--13 months (the period of observation). The stability of infectivity of lyophilized cell culture virus preparations was similar to that of allantoic virus preparations.
D000482:Allantois; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D000956:Antigens, Viral / Q000032:analysis; D002417:Cattle; D002642:Chick Embryo; D003168:Complement Fixation Tests; D046508:Culture Techniques; D005612:Freeze Drying; D007668:Kidney; D009975:Orthomyxoviridae / Q000254:growth & development* / Q000276:immunology; D011817:Rabbits; D014776:Virus Cultivation / Q000379:methods*; D014779:Virus Replication
D000914:Antibodies, Viral; D000956:Antigens, Viral