The determination of gamma-glutamyl transpeptidase by reaction rate assay at 37 degrees C.
The determination of gamma-glutamyl transpeptidase by a reaction rate assay using optimal reaction conditions at 37 degrees is described. Specific conditions and instrument settings are described for the LKB Reaction Rate Analyser but the actual assay conditions are applicable to any similar reaction rate system. The precision of the method has been evaluated and a reference range for normal male (less than 45 U/l) and female (less than 30 U/l) subjects determined.
D000813:Anilides; D002021:Buffers; D005260:Female; D006033:Glycylglycine; D006461:Hemolysis; D006493:Heparin; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008297:Male; D011409:Propylene Glycols; D012737:Sex Factors; D013053:Spectrophotometry / Q000379:methods; D013696:Temperature; D014325:Tromethamine; D005723:gamma-Glutamyltransferase / Q000097:blood*
D000813:Anilides; D002021:Buffers; D011409:Propylene Glycols; D014325:Tromethamine; D006033:Glycylglycine; D006493:Heparin; D005723:gamma-Glutamyltransferase