The effect of inorganic phosphate on cyanogenesis by Pseudomonas aeruginosa.
The biosynthesis of hydrogen cyanide (HCN) by a strain of Pseudomonas aeruginosa is found to be significantly influenced by inorganic phosphate. Optimum HCN production occurs when the phosphate concentration is between 1 and 10 mM. Above and below this concentration the amount of HCN produced decreases sharply and at 0.1 and 100 mM phosphate low HCN production occurs. If a culture growing at 0.1 mM phosphate and producing low HCN is shifted to 10 mM phosphate, HCN biosynthesis resumes. Experiments with chloramphenicol indicate that de novo-protein synthesis is required for the process.
D001426:Bacterial Proteins / Q000096:biosynthesis; D002701:Chloramphenicol / Q000494:pharmacology; D006856:Hydrogen Cyanide / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D010710:Phosphates / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000378:metabolism*
D001426:Bacterial Proteins; D010710:Phosphates; D006856:Hydrogen Cyanide; D002701:Chloramphenicol