Poly(A) polymerases of rat liver nuclei. Purification and specificity.
Two poly(A) polymerases were isolated from rat liver nuclei and purified more than one thousand times by ion exchange chromatography on DEAE-Sephadex and phosphocellulose columns as well as affinity chromatography on a chromosomal RNA-Sepharose column. One of the two enzymes is bound to chromatin and uses as primer chromosomal RNA, while the second one is localized in the nucleoplasm and uses as primer poly(A) and hnRNA isolated from chromatin. The two enzymes seem to participate in the polyadenylation of chromosomal RNA in vitro, by a coupled mechanism. According to this mechanism, the chromatin bound enzyme adds 120-130 adenosine nucleotides to chromosomal RNA and consequently the nucleoplasmic enzyme completes the poly-adenylation by adding 80-90 more AMP units to the polyadenylated end of chromosomal RNA.
D000818:Animals; D002413:Cations, Divalent / Q000494:pharmacology; D002467:Cell Nucleus / Q000201:enzymology*; D002843:Chromatin / Q000378:metabolism*; D002845:Chromatography / Q000379:methods; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000648:ultrastructure; D008297:Male; D008970:Molecular Weight; D009713:Nucleotidyltransferases / Q000302:isolation & purification*; D009994:Osmolar Concentration; D011062:Polynucleotide Adenylyltransferase / Q000302:isolation & purification* / Q000378:metabolism; D012313:RNA / Q000378:metabolism*; D051381:Rats; D013379:Substrate Specificity
D002413:Cations, Divalent; D002843:Chromatin; D012313:RNA; D009713:Nucleotidyltransferases; D011062:Polynucleotide Adenylyltransferase