Isolation of 3-phosphohistidine from phosphorylated pyruvate, phosphate dikinase.
Pyruvate, phosphate dikinase (EC 2-7-9-1) catalyzes formation of phosphoenolpyruvate, AMP, and inorganic pyrophosphate from pyruvate, ATP, and orthophosphate. A pyrophosphoryl and phosphoryl form of the enzyme is involved in this transfer. The [32P]phosphoryl form of pyruvate, phosphate dikinase was prepared with enzyme isolated from Bacteroides symbiosus. The [32P]phosphoryl enzyme was found to have properties corresponding to a phosphoramidate linkage and this was confirmed by isolation of 3-[32P]phosphohistidine from alkaline hydrolysates of the enzyme. The histidyl residue is considered to be the pyrophosphoryl- and phosphoryl-carrier between the three substrate sites of this enzyme.
D001439:Bacteroides / Q000201:enzymology; D001665:Binding Sites; D002384:Catalysis; D006639:Histidine / Q000031:analogs & derivatives* / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D006898:Hydroxylamines / Q000494:pharmacology; D007700:Kinetics; D010710:Phosphates / Q000032:analysis*; D010770:Phosphotransferases / Q000032:analysis*; D011725:Pyridines / Q000494:pharmacology; D011772:Pyruvate, Orthophosphate Dikinase / Q000032:analysis*
D006898:Hydroxylamines; D010710:Phosphates; D011725:Pyridines; D006639:Histidine; D010770:Phosphotransferases; D011772:Pyruvate, Orthophosphate Dikinase