A simple method for the cryopreservation of lymphocytes. Retention of specific immune effector functions by frozen-stored cells.
A simple, quick and inexpensive method for cryostorage of lymphocytes is discribed. When injected into appropriate normal recipients, frozen-stored rat and sheep lymphocytes caused GVH and NLT reactions respectively. Sheep lymphocytes remained viable after several months storage and functioned satisfactorily as 51Cr-labelled target cells in assays for cytotoxic antibodies. Lymphocytes, including immunoblasts, drained from sheep efferent lymphatics during immune responses to injected murine P815 cells or allogeneic lymphocytes survived freezing; when thawed the cells retained specific immune cytotoxic effector functions including the ability to secrete complement-dependent and leucocyte-dependent antibodies
D000818:Animals; D000906:Antibodies; D000921:Antibody-Producing Cells; D001793:Blood Preservation / Q000379:methods*; D002470:Cell Survival; D003601:Cytotoxicity Tests, Immunologic; D005615:Freezing; D006087:Graft vs Host Reaction; D006650:Histocompatibility Testing; D007958:Leukocyte Count; D008214:Lymphocytes / Q000276:immunology; D051381:Rats; D012756:Sheep; D013997:Time Factors
D000906:Antibodies