Studies on soybean trypsin inhibitors. XIII. Preparation and characterization of active fragments from Bowman-Birk proteinase inhibitor.
Soybean Bowman-Birk inhibitor, a double-headed inhibitor of trypsin and alpha-chymotrypsin, was treated with cyanogen bromide and then pepsin to yield two inhibitory active fragments. Structural investigation showed that one of the fragments was derived from the trypsin inhibitory domain and the other from the chymotrypsin inhibitory domain of the inhibitor. In contrast to the unusual stability of the native inhibitor, the separated domains were less stable and could be inactivated with excess proteinases. These results suggest that the legume double-headed inhibitors acquired their unusual stability by duplicating an ancestral single-headed structure.
D000595:Amino Acid Sequence; D002918:Chymotrypsin / Q000037:antagonists & inhibitors; D006863:Hydrogen-Ion Concentration; D010434:Pepsin A / Q000378:metabolism; D010446:Peptide Fragments / Q000494:pharmacology; D013329:Structure-Activity Relationship; D013820:Thermolysin / Q000378:metabolism; D014358:Trypsin Inhibitor, Bowman-Birk Soybean / Q000494:pharmacology; D014361:Trypsin Inhibitors / Q000494:pharmacology
D010446:Peptide Fragments; D014358:Trypsin Inhibitor, Bowman-Birk Soybean; D014361:Trypsin Inhibitors; D002918:Chymotrypsin; D010434:Pepsin A; D013820:Thermolysin