Derivatization to stabilize some aliphatic primary hydroxylamines for g.l.c. analysis.
By appropriate choice of trimethylsilylating and trifluoroacetylating reagents and organic solvents for extraction, stable derivative of aliphatic primary hydroxylamines metabolites, N-hydroxyphentermine, N-hydroxychlorphentermine, N-hydroxymexiletene, N-hydroxyphenethylamine, N-hydroxyamphetamine, and N-hydroxy-3,4-dimethoxyamphetamine, were obtained and examined by g.l.c. analysis without decomposition and without interference from the parent drug or other metabolic products.
D000588:Amines / Q000032:analysis; D000662:Amphetamines / Q000032:analysis; D002849:Chromatography, Gas; D005463:Fluoroacetates; D006898:Hydroxylamines / Q000032:analysis* / Q000652:urine; D008099:Liver / Q000032:analysis; D013058:Mass Spectrometry; D008722:Methods; D010091:Oximes / Q000032:analysis; D014269:Trifluoroacetic Acid / Q000138:chemical synthesis; D014297:Trimethylsilyl Compounds / Q000138:chemical synthesis
D000588:Amines; D000662:Amphetamines; D005463:Fluoroacetates; D006898:Hydroxylamines; D010091:Oximes; D014297:Trimethylsilyl Compounds; D014269:Trifluoroacetic Acid