The isolation and identification of 2,4-diketones present in human and animal tissues and in urine.
The chemical isolation of 2,3-diketone fraction from hydrolysates of various mammalian tissues has been accomplished by the use of a modified Girard T procedure. This fraction, which constitutes a new lipid class, has been resolved by gas chromatography into a number of homologous 2,4-diketones, ranging in chain length from C13 to C25. After separation by preparative gas chromatography, the following compounds have been unequivocally identified: 2,4-heptadecanedione, 2,4-nonadecanedione, 2,4-heneicosanedione, 2,4-docosanedione, and delta12-2,4-heneicosenedione. The 2,4-diketones appear to exist in tissues in the free state or in labile combination. They are present also in human urine.
D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002614:Chelating Agents; D002849:Chromatography, Gas / Q000379:methods; D006634:Histamine H1 Antagonists; D006801:Humans; D007659:Ketones / Q000032:analysis* / Q000302:isolation & purification / Q000652:urine; D008055:Lipids; D008099:Liver / Q000032:analysis*; D014018:Tissue Distribution
D002614:Chelating Agents; D006634:Histamine H1 Antagonists; D007659:Ketones; D008055:Lipids