Properties of amylase-linked immunoglobulins.
In this report, the properties of eight cases of amylase-linked immunoglobulins were gel filtration using Sephadex G-200 superfine. The class of heavy chain of amylase-linked immunoglobulins was proved to be gamma in four cases and alpha in three cases by immunoelectrophoresis followed by amylase activity staining. In one of the cases, the precipitin line against light chain lambda could be visualized only after treatment with 0.1 M 2-mercaptoethanol. Then, the type of light chain was determined to be exclusively lambda irrespective of heavy chain classes. In two cases, the immunoglobulin complexes were partially dissociated into normal amylase and immunoglobulin G at pH 8.6, and completely dissociated at pH 9.0. In three cases, the complexes were completely dissociated at pH 8.6. The precipitin line of papain treated amylase-linked immunoglobulin G against anti-Fc was not. This fact suggests that the binding site of amylase-linked immunoglobulins G was located in the Fab portion of immunoglobulin molecule and that the complexes are specific antigen-antibody complexes. Treatment with Con-A Sepharose caused the dissociation of the amylase-immunoglobulin complexes. It is suggested that the changes in the conformation of amylase-linked immunoglobulin causes the dissociation of this immuno-complex. Thus, it is elucidated that the complexes of amylase and immunoglobulins are specific antigen-antibody complexes, and that these complexes must be recognized as one of the circulating autoantibodies in plasma, and must be clearly distinguished from the other unknown macromolecular amylase complexes.
D000681:Amylases / Q000097:blood*; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D003208:Concanavalin A; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G; D007136:Immunoglobulins / Q000032:analysis*; D008297:Male; D010179:Pancreas / Q000201:enzymology; D010206:Papain; D011485:Protein Binding; D012463:Saliva / Q000201:enzymology; D012685:Sepharose
D007074:Immunoglobulin G; D007136:Immunoglobulins; D003208:Concanavalin A; D012685:Sepharose; D000681:Amylases; D010206:Papain