Degradation of arylsulfate by hepatic microsomes.
The enzyme liberated by some treatments and the changes in arylsulfatase C activity in chronic hepatic damage were investigated in rat liver. 1. The enzyme activity liberated by ultrasound was the highest in the conditions studied. 2. Arylsulfatase C was assayed using p-nitrophenyl sulfate in 0.25 M Tris/acetate buffer as substrate. It is shown that this method can be used to measure arylsulfatase C activity in a mixture of arylsulfatases A and B. 3. The enzyme is mainly located in the microsomal fraction in rat liver. In toxic hepatic damage, the enzyme activity decreases from the early stage; decreasing markedly in chronic hepatic damage. The activity seems to reflect damage to the microsomes and therefore arylsulfatase C activity can be a good indicator of injury to liver microsomes.
D000818:Animals; D001192:Arylsulfatases / Q000378:metabolism*; D002553:Cerebroside-Sulfatase / Q000378:metabolism; D002803:Chondro-4-Sulfatase / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008099:Liver / Q000201:enzymology / Q000293:injuries; D008247:Lysosomes / Q000201:enzymology; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D009928:Organ Specificity; D051381:Rats; D013429:Sulfatases / Q000378:metabolism*; D013997:Time Factors; D014465:Ultrasonics
D013429:Sulfatases; D001192:Arylsulfatases; D002553:Cerebroside-Sulfatase; D002803:Chondro-4-Sulfatase