Autolysis of high-GC isolates of Pseudomonas putrefaciens.
High-GC isolates of P. putrefaciens undergo extensive autolysis after growth, resulting in a marked decrease in turbidity and the release of high-molecular-weight DNA which imparts a high viscosity to culture broths. The native DNA released is resistant to attack by the exocellular DNase activity of the culture broths. Autolysis is inhibited by a pH of 6.0 and the presence of 0.001 M Mg++ or Ca++, and is enhanced by elevated pH values and temperatures. This autolytic phenomenon in broth cultures readily distinguishes high- from low-GC isolates. The latter do not exhibit autolysis.
D002118:Calcium / Q000494:pharmacology; D002455:Cell Division; D002470:Cell Survival / Q000187:drug effects*; D004269:DNA, Bacterial / Q000378:metabolism; D003851:Deoxyribonucleases; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000494:pharmacology; D011549:Pseudomonas / Q000187:drug effects / Q000378:metabolism*; D013045:Species Specificity
D004269:DNA, Bacterial; D003851:Deoxyribonucleases; D008274:Magnesium; D002118:Calcium