A procedure for the assay of DNA damage in mammalian cells by alkaline elution and microfluorometric DNA determination.
The microfluorimetric assay of Kissane and Robins (J. Biol. Chem. 233, 184-188, 1958) has been modified to monitor DNA content in alkaline elution fractions. The elution profiles of DNA from treated and control cultured cells demonstrated a good correlation whether DNA in the elution fractions was determined by the fluorometric method or by measuring its radioactivity. The DNA recovery was of about 80% and the measured material was DNase digestible.
D002460:Cell Line; D055598:Chemical Phenomena; D002621:Chemistry; D004247:DNA / Q000032:analysis* / Q000096:biosynthesis / Q000302:isolation & purification; D003851:Deoxyribonucleases; D005470:Fluorometry / Q000379:methods; D006863:Hydrogen-Ion Concentration; D013936:Thymidine / Q000378:metabolism
D004247:DNA; D003851:Deoxyribonucleases; D013936:Thymidine