Fluorometric determination of nadolol in human serum and urine.
To determine nadolol, a new beta-adrenergic blocking agent, in serum and urine, the drug is extracted into n-butyl acetate or ether from alkaline potassium chloride saturated samples. After back-extraction into 0.1 N HCl, the drug is oxidized with periodic acid; the resulting aldehyde is coupled with o-phenylenediamine to produce a fluorescent compound. The method can measure as little as 0.01 microgram of nadolol/sample.
D000319:Adrenergic beta-Antagonists / Q000032:analysis* / Q000097:blood / Q000652:urine; D006801:Humans; D008297:Male; D008722:Methods; D010084:Oxidation-Reduction; D011412:Propanolamines / Q000032:analysis* / Q000097:blood / Q000652:urine; D013050:Spectrometry, Fluorescence
D000319:Adrenergic beta-Antagonists; D011412:Propanolamines