Single-mouse urine collection and pH monitoring system.
A glass cage with minimal surface area was designed and used to house mice for 24-hour urine collections. An experiment was performed with a radio-labeled compound excreted in the urine to assess the collection efficiency of the cage. In this experiment 74.2 +/- 6.5% of the excreted radioactivity was recovered in the urine, with 25.8 +/- 6.5% found adhering to the cage surfaces. When a flow-through pH electrode, meter, and recorder were attached, the system provided a continuous pH versus time urination record.
D000818:Animals; D005260:Female; D006799:Housing, Animal; D006863:Hydrogen-Ion Concentration; D051379:Mice; D008815:Mice, Inbred Strains / Q000652:urine*; D013048:Specimen Handling / Q000295:instrumentation / Q000662:veterinary*; D013696:Temperature