[Properties and isozymes of acid and alkaline phosphatase in cattle testes and endometrium].
The activity of acid phosphatase in the testicular parenchyma of cattle was found to be higher than that in the endometrium. The acid phosphatase of either tissue broke up p-nitrophenylphosphate at a rate twice as high as that reached for di-sodium phenylphosphate. Hydrolysis of phenolphthalein diphosphate was slower and that of beta-glycerophosphate very slow. Two optimum pH values and two isozymes were recordable from acid phosphatase in testicular supernatant and three optimum pH values as well as three isozymes from that in endometrial supernatant. Even concentrations pf 0.2 mM of copper and mercury ions were strongly inhibiting the acid phosphatase, whereas lead and cadmium ions proved less effective. The activity of acid phosphatase from testes dropped by 24 per cent and that from the endometrium by 16 per cent over eight weeks of storage at -20 degrees C. The average activity of alkaline phosphatase in the endometrium was much higher than that in testicular parenchyma. Alkaline phosphatase from testes exhibited the shortest break-up time for p-nitrophenylphosphate, whereas disodium-phenylphosphate was broken up at the highest rate by endometrial alkaline phosphatase. Two isozymes of alkaline phosphatase were recordable each from the testes and endometrium.
D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002417:Cattle; D004717:Endometrium / Q000201:enzymology*; D005260:Female; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000378:metabolism; D008297:Male; D010649:Phenylalanine; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D013696:Temperature; D013737:Testis / Q000201:enzymology*
D007527:Isoenzymes; D010649:Phenylalanine; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D010744:Phosphoric Monoester Hydrolases
Untersuchungen über einige Eigenschaften und über die Isozyme der sauren und alkalischen Phosphatase im Hoden und in der Uterusschleimhaut beim Rind.