Enzymatic hydrolysis of agar: purification and characterization of neoagarobiose hydrolase and p-nitrophenyl alpha-galactoside hydrolase.
The mixture of polysaccharides in the gelling component of agar (agarose) is hydrolyzed to D-galactose and 3,6-anhydro-L-galactose by a series of hydrolytic enzymes obtained from Pseudomonas atlantica. The final degradative step in the pathway of agarose decomposition is the hydrolysis of the alpha-linkage in the dissaccharide neoagarobiose yielding D-galactose and 3,6-anhydro-L-galactose. Pseudomonas atlantica when grown on agar produces two specific enzymes, p-nitrophenyl alpha-galactose hydrolase and neoagarobiose hydrolase. The purification and partial characterization of both enzymes are presented.
D000362:Agar / Q000378:metabolism*; D000645:Ammonium Sulfate; D002462:Cell Membrane / Q000201:enzymology; D002474:Cell-Free System; D011232:Chemical Precipitation; D006026:Glycoside Hydrolases / Q000302:isolation & purification / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008094:Lithium / Q000494:pharmacology; D008970:Molecular Weight; D009598:Nitrophenylgalactosides / Q000378:metabolism; D011134:Polysaccharides; D011188:Potassium / Q000494:pharmacology; D011549:Pseudomonas / Q000201:enzymology* / Q000378:metabolism; D012623:Seawater; D012964:Sodium / Q000494:pharmacology; D014871:Water Microbiology
D011134:Polysaccharides; D009598:Nitrophenylgalactosides; D000362:Agar; D008094:Lithium; D012964:Sodium; D006026:Glycoside Hydrolases; D011188:Potassium; D000645:Ammonium Sulfate