Correlation between the exposure of aromatic chromophores at the surface of the Fc domains of immunoglobulin G and their ability to bind complement.
The recognition that certain biological effector functions associated with the Fc region of human IgG are mediated exclusively by either the Cgamma2 or Cgamma3 domains prompted a study of some of the physical properties of the isolated domains in an attempt to correlate these with functional differentiation. The degree of aromatic chromophore exposure of intact Fc and fragments corresponding to the Cgamma2 and Cgamma3 domains were determined by solvent perturbation difference spectroscopy using 20% ethylene glycol. For the monomeric Cgamma2 fragment one of the two tryptophans and all four of the tyrosines were exposed to solvent. In the pFc' fragment, which represented a dimer of two intact Cgamma3 domains, an average of 0.4 of the two tryptophans of 3.3 of the five tyrosines per chain were exposed. These data were consistent with the suggested involvement of tryptophan in complement fixation since Cgamma2 binds C1q but pFc' does not. Several fragments derived from the Cgamma3 region had previously been shown to have differing environments for their aromatic side chains from circular dichroism studies. These fragments have now been shown to exhibit different degrees of chromophore exposure to solvent. Removal of the carboxy-termimal heptapeptide from the intact, Cgamma3 domain resulted in a fragment not only showing a greater exposure of aromatic residues but also having the ability to bind Clq. Our data suggest that the structural requirements for C1Q binding may be quite commonplace within Fc, but tertiary folding limits their expression except in Cgamma2 in the native molecule. The solvent perturbation observed with Fc was somewhat lower than would have been expected from the results with the isolated domains, suggesting that interdomain interactions may result in burial of aromatic residues.
D001665:Binding Sites; D003172:Complement C1 / Q000378:metabolism*; D003165:Complement System Proteins / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007141:Immunoglobulin Fc Fragments / Q000378:metabolism; D007074:Immunoglobulin G / Q000378:metabolism; D007457:Iodine Radioisotopes; D007784:Lactoperoxidase; D010434:Pepsin A; D011487:Protein Conformation; D011489:Protein Denaturation; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D013057:Spectrum Analysis; D013329:Structure-Activity Relationship; D014364:Tryptophan; D014443:Tyrosine
D003172:Complement C1; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D007457:Iodine Radioisotopes; D012997:Solvents; D014443:Tyrosine; D014364:Tryptophan; D003165:Complement System Proteins; D007784:Lactoperoxidase; D010434:Pepsin A