Influence of thiocyanate ions on starch-iodine reaction used for the estimation of alpha-amylase activity.
A strong simulating effect of thiocyanate ions during the estimation of alpha-amylase by the amyloclastic method is shown. Thiocyanate ions themselves catalyse the decolorization of the starch-iodine complex. This may lead to wrong values during determination of alpha-amylase by this method. The rate of thiocyanate-iodine reaction depends upon the hydrogen ion concentration of the iodine reagent.
D000681:Amylases / Q000378:metabolism*; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007455:Iodine; D008027:Light; D012463:Saliva / Q000032:analysis; D012907:Smoking / Q000378:metabolism; D013053:Spectrophotometry; D013213:Starch; D013861:Thiocyanates / Q000032:analysis; D013997:Time Factors; D000516:alpha-Amylases / Q000378:metabolism*
D013861:Thiocyanates; D013213:Starch; D007455:Iodine; D000681:Amylases; D000516:alpha-Amylases