A rapid fluorometric assay of angiotensin-converting enzyme.
A rapid, simple and sensitive method is described for the fluorometric assay of angiotesin-converting enzyme using Fluorescamine. The critical factors such as optimal pH, incubation time, chloride ion, and inactivation by EDTA and 8-hydroxyquinoline were examined. The Km value for hippuryl-L-histidyl-L-leucine was 0.5 mM. This method was applied to the assay of angiotensin-converting enzyme in the rat serum and the reproducible values were obtained with a 10 mul of the rat serum.
D000818:Animals; D005450:Fluorescamine; D005470:Fluorometry / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007930:Leucine; D008168:Lung / Q000201:enzymology; D007703:Peptidyl-Dipeptidase A / Q000032:analysis*; D051381:Rats; D013552:Swine
D005450:Fluorescamine; D007703:Peptidyl-Dipeptidase A; D007930:Leucine