Interaction of plasma membranes with influenza virus. VII. Effect on 3',5'-cyclic adenosine monophosphate phosphodiesterase activity.
In chick embryo cells (CEC) and plasma membranes (PM) isolated therefrom, three forms of 3',5'-cyclic adenosine monophosphate (cAMP) phosphodiesterase (PDE) were demonstrated: (1) PDE activated only by Ca2+ (Ca-PDE); (2) PDE activated only by Mg2+ (Mg-PDE); and (3) PDE stimulated in the presence of 2 mM Mg2+ by low concentrations of Ca2+ (Ca-Mg-PDE). Purified influenza A virus, under suitable conditions, lowered the activities of these PDEs. The decrease was greater in samples incubated in the presence of adenosine triphosphate (ATP) than in those incubated in its absence.
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002462:Cell Membrane / Q000201:enzymology; D002642:Chick Embryo; D046508:Culture Techniques; D000242:Cyclic AMP / Q000378:metabolism; D004789:Enzyme Activation; D009980:Influenza A virus / Q000254:growth & development*; D008274:Magnesium / Q000494:pharmacology; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*
D000255:Adenosine Triphosphate; D000242:Cyclic AMP; D010727:Phosphoric Diester Hydrolases; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D008274:Magnesium; D002118:Calcium