Pharmacologic responses of cells of a neuroblastoma X glioma hybrid clone and modulation of synapses between hybrid cells and mouse myotubes.
Cells of the hybrid clone NG108-15 responded to 5-hydroxytryptamine (5-HT), dopamine or acetylcholine with graded depolarizations involving membrane conductance increases. Responses desensitized during continuous application of the neurotransmitters, and responses to 5-HT and dopamine cross-desensitized: a desensitizing application of one neurotransmitter also desensitized the hybrd cell to the other neurotransmitter. 5-HT and acetylcholine did not cross-desensitize. The hybrid cell 5-HT response was not attenuated by D-LSD, and was blocked by 10(-5) M morphine, although not via binding to naloxone-sensitive opiate receptors. 5-HT or the prostaglandin PGF2alpha caused the release of acetylcholine at the synapses of hybrid cells with mouse myotubes. Application of 5-HT or PGF2alpha also facilitated the synaptic release elicited by hybrid cell action potentials. Following treatment with the antimitotic agent cytosine arabinoside, co-cultures of hybrid cells and mouse myotubes exhibited plentiful synaptic connections only if maintained in medium containing 1 mM dibutyryl cAMP (dBcAMP). After X-irradiation, co-cultures were synaptically active even in the absence of dBcAMP. Thus, methods have been found to regulate both the short-term and long-term synaptic activity of NG108-15 hybrid cells.
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002999:Clone Cells; D004298:Dopamine / Q000494:pharmacology; D005910:Glioma / Q000503:physiopathology*; D008564:Membrane Potentials / Q000187:drug effects; D051379:Mice; D009020:Morphine / Q000494:pharmacology; D009374:Neoplasms, Experimental / Q000503:physiopathology; D009431:Neural Conduction / Q000187:drug effects; D009447:Neuroblastoma / Q000503:physiopathology*; D009469:Neuromuscular Junction / Q000187:drug effects*; D018377:Neurotransmitter Agents / Q000494:pharmacology*; D011460:Prostaglandins F / Q000494:pharmacology; D011957:Receptors, Opioid / Q000187:drug effects; D012701:Serotonin / Q000494:pharmacology; D013569:Synapses / Q000187:drug effects*
D018377:Neurotransmitter Agents; D011460:Prostaglandins F; D011957:Receptors, Opioid; D012701:Serotonin; D003994:Bucladesine; D009020:Morphine; D000109:Acetylcholine; D004298:Dopamine