Isolation and partial characterization of trehalase from Ascaris muscle.
The tissues of female Ascaris suum were assayed for alpha,apha'-glucoside 1-D-glucohydrolase (trehalase) activity. A soluble from of the enzyme was isolated from muscle tissue and purified approximately 37-fold. The enzyme was specific for trehalose as substrate. The pH optimum for enzymatic activity was found to be 6.0, and the apparent Km for trehalose was estimated to be 2.1 x 10-4 M. The product of the reaction was identified as D-glucose by chemical, chromatographic and enzymatic methods.
D001200:Ascaris / Q000201:enzymology*; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004187:Disaccharides / Q000302:isolation & purification*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009132:Muscles / Q000201:enzymology; D013379:Substrate Specificity; D014199:Trehalose / Q000302:isolation & purification*
D004187:Disaccharides; D014199:Trehalose