[Template chromatography on immobilized oligonucleotides. Synthesis and application of oligodeoxyadenosine-5'-phosphate--DEAE-cellulose (author's transl)].
Oligomers of deoxyadenylic acid, obtained by polycondensation, were covalently attached to polyvinyl alcohol. These polymer-bound oligonucleotides undergo very strong adsorption on DEAE-cellulose such that at neutral pH and with 1 M NaCl, partial desorbtion occurs only above 60 degrees. The use of this PV(pA)n-DEAE-cellulose for the column chromatographic separation of deoxythymidylic acid oligomers obtained by polycondensation, according to the principle of base-pairing, is discussed. Linear oligomers and also pyrophosphate derivatives of thymidylic acid which contain more than five monomer units undergo strong retardation under the conditions of base-pairing. Cyclic oligonucleotides do not show any noticeable interaction with the stationary phase. Thus, through the use of a temperature gradient, it is possible to fractionally separate the polycondensate, giving an average degree of polymerisation of 6--10.
D000327:Adsorption; D002848:Chromatography, DEAE-Cellulose / Q000379:methods*; D003839:Deoxyadenosines; D003854:Deoxyribonucleotides; D006863:Hydrogen-Ion Concentration; D009712:Nucleotides, Cyclic; D009841:Oligonucleotides / Q000138:chemical synthesis; D011142:Polyvinyl Alcohol; D013696:Temperature; D013938:Thymidine Monophosphate
D003839:Deoxyadenosines; D003854:Deoxyribonucleotides; D009712:Nucleotides, Cyclic; D009841:Oligonucleotides; D013938:Thymidine Monophosphate; D011142:Polyvinyl Alcohol
Template-chromatographie an stationär gebundenen oligonukleotiden syntheses und anwendung von oligodesoxyadenosin-5'-phosphat-deae-zellulose