[Approach to a practical method for screening and identifying microorganism genera from urine (author's transl)].
In this study the author reported upon a practical new system for screening and identifying the microbial agents causing urinary tract infections. This system is composed of a combination of 3 screening procedures (pH-value + nitrite-test + catalase-test) and 8 selective culture media for the purpose of genus identification within 24 hours (Uripret-G). A total of 130 cultures was investigated. The employed microorganisms were mainly recovered from urine samples. They included the following species: Candida albicans, Citrobacter freundii, Enterobacter aerogenes, Enterobacter cloacae, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Proteus inconstans, Proteus mirabilis, Proteus morganii, Proteus rettgeri, Proteus vulgaris, Serratia liquefaciens, Serratia marcescens, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus faecalis and Streptococcus faecium. Employing coded cultures not only monoinfections but also multiinfections in urine samples were simulated. Under the circumstances of investigation it was possible with the help of the new system to reidentify the genera of all but two of the 130 employed microorganisms.
D001431:Bacteriological Techniques; D002176:Candida albicans / Q000302:isolation & purification; D002374:Catalase / Q000652:urine; D002954:Citrobacter / Q000302:isolation & purification; D003470:Culture Media; D004755:Enterobacteriaceae / Q000302:isolation & purification; D004926:Escherichia coli / Q000302:isolation & purification; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007711:Klebsiella pneumoniae / Q000302:isolation & purification; D009573:Nitrites / Q000652:urine; D011511:Proteus / Q000302:isolation & purification; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification; D012705:Serratia / Q000302:isolation & purification; D013210:Staphylococcus / Q000302:isolation & purification; D013291:Streptococcus / Q000302:isolation & purification; D014552:Urinary Tract Infections / Q000209:etiology*; D014556:Urine / Q000382:microbiology*
D003470:Culture Media; D009573:Nitrites; D002374:Catalase
Praxisnahe Verfahren zur Erfassung und Bestimmung von Mikroorganismengattungen im Urin