Determination of flunitrazepam, desmethylflunitrazepam and clonazepam in plasma by gas liquid chromatography with an internal standard.
A method is developed for gas-chromatographic determination of flunitrazepam, desmethylflunitrazepam and clonazepam in plasma, using methylclonazepam as internal standard. Following extraction of the benzodiazepines and their hydrolysis, the benzophenones are analyzed by gas-chromatography, with a glass column filled with 3% OV-225 on Gas Chrom Q and a 63Ni electron capture detector. The technique has a good selectivity. Its limit of sensitivity is about 0.5 ng/ml of plasma. It is suitable for investigations on pharmacokinetics and bioavailability as well as for monitoring the correlation between plasma level and therapeutic efficacy and toxicology.
D000284:Administration, Oral; D014151:Anti-Anxiety Agents / Q000097:blood*; D001570:Benzodiazepinones / Q000097:blood*; D001711:Biotransformation; D002648:Child; D002849:Chromatography, Gas; D002998:Clonazepam / Q000008:administration & dosage / Q000097:blood*; D003640:Dealkylation; D005445:Flunitrazepam / Q000008:administration & dosage / Q000097:blood*; D006801:Humans; D007273:Injections, Intramuscular; D007275:Injections, Intravenous; D007700:Kinetics; D008722:Methods
D014151:Anti-Anxiety Agents; D001570:Benzodiazepinones; D002998:Clonazepam; D005445:Flunitrazepam