Trisialoganglioside synthesis by a chicken brain sialyltransferase. Comparative study with the similar reaction for the synthesis of disialoganglioside.
An enzyme preparation from embryonic chicken brain catalyzes the transfer of sialic acid from CMP-N-acetylneuraminic acid to ceramide-Glc-Gal(NeuAc-NeuAc)-GalNAc-Gal (GDlb) to form ceramide-Glc-Gal(NeuAc-NeuAc)-GalNAc-Gal-NeuAc (GTlb). The sialyltransferase activity was measured during the development of the embryo, the subcellular distribution of this activity was determined and several kinetic properties of the reaction were examined. A comparative study with the similar reaction involved in the transfer of sialic acid to the terminal galactose in ceramide-Glc-Gal(NeuAc)-GalNAc-Gal (GMl) was made. The results obtained in this comparative study suggest that the transfer of sialic acid in both reactions is catalyzed by the same enzyme.
D000375:Aging; D000818:Animals; D001921:Brain / Q000196:embryology / Q000201:enzymology*; D002417:Cattle; D002642:Chick Embryo; D002645:Chickens; D005732:Gangliosides / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D011092:Polyethylene Glycols / Q000494:pharmacology; D011136:Polysorbates / Q000494:pharmacology; D012799:Sialyltransferases / Q000378:metabolism*; D013329:Structure-Activity Relationship; D013347:Subcellular Fractions / Q000201:enzymology; D014166:Transferases / Q000378:metabolism*
D005732:Gangliosides; D011136:Polysorbates; D011092:Polyethylene Glycols; D014166:Transferases; D012799:Sialyltransferases