[Purification of rat brain guanylate cyclase].
The purification of guanylate cyclase has been achieved. After electrophoresis on polyacrylamide gel only one protein band was observed. The low factor of purification must therefore be ascribed to the loss of an activator of guanylate cyclase. Stimulation of the activity by nitroprusside is also lost during purification. The purified enzyme follows Michaelis--Menten kinetics, it is activated by Mn++ ions and inhibited by triphospho nucleotides.
D000818:Animals; D001921:Brain / Q000201:enzymology*; D004789:Enzyme Activation; D006162:Guanylate Cyclase / Q000302:isolation & purification*; D007700:Kinetics; D008970:Molecular Weight; D009599:Nitroprusside / Q000494:pharmacology; D051381:Rats
D009599:Nitroprusside; D006162:Guanylate Cyclase
Purification de la guanylate cyclase du cerveau de Rat.