Fibrin plate method with reagents purified by affinity chromatography and its use for determination of fibrinolytic and other proteolytic activity in saliva, bile and plasma.
A modification of the fibrin plate method is presented. Plasminogen-free human fibrinogen and plasminogen purified by affinity chromatography have been used. Fibrin plates without and with a constant amount of plasminogen and with agarose as stabilizing medium were used for the estimation of plasmin and plasminogen activator activity. Activator activity could be demonstrated in sterile bile and saliva. When plasmin activity was present, estimations of plasminogen activator were approximate. The method is sensitive, small volumes of reagents and samples are needed. The error of the method is comparatively low and the reproducibility is good.
D001646:Bile / Q000032:analysis*; D002846:Chromatography, Affinity; D005340:Fibrinogen / Q000032:analysis; D005341:Fibrinolysin / Q000032:analysis*; D005342:Fibrinolysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008722:Methods; D010949:Plasma / Q000032:analysis*; D010958:Plasminogen / Q000032:analysis; D010960:Plasminogen Activators / Q000032:analysis*; D012463:Saliva / Q000032:analysis*; D013917:Thrombin / Q000032:analysis
D007202:Indicators and Reagents; D005340:Fibrinogen; D010958:Plasminogen; D010960:Plasminogen Activators; D013917:Thrombin; D005341:Fibrinolysin