pH-dependent changes of 2,3-bisphosphoglycerate.
A systematic study of the pH dependent changes in the range 6.6--7.4 of 2,3 bisphosphoglycerate (2,3-DPG) was performed in the presence and absence of glucose during transitional and steady states. The results indicate that 2,3-DPGase breaks down 2,3-DPG nealy independent of pH at a rate of 480 mu moles 2,3-DPG/1 cells.h. The 2,3-DPG mutase is practically completely inhibited below pH 6.9. The 2,3-DPG level in the presence of glucose reaches a pH dependent steady state after about 18 h. The share of the 2,3-DPG bypass in the steady state decreases from 24% at pH 7.4 to 12% at pH 7.0. The formation of pyruvate corresponds to the beadkdown of 2,3-DPG after consumption of an unknown reducing substance.
D000255:Adenosine Triphosphate / Q000097:blood; D001731:Bisphosphoglycerate Mutase / Q000097:blood; D001786:Blood Glucose / Q000378:metabolism; D004912:Erythrocytes / Q000201:enzymology / Q000378:metabolism; D005994:Glycerophosphates / Q000097:blood*; D006019:Glycolysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007773:Lactates / Q000097:blood
D001786:Blood Glucose; D005994:Glycerophosphates; D007773:Lactates; D000255:Adenosine Triphosphate; D001731:Bisphosphoglycerate Mutase