A clinical method for the determination of serum gamma-glutamyl transpeptidase.
A simple, highly sensitive and reproducible method for the assay of gamma-glutamyl transpeptidase (EC 2.3.2-) activity is introduced, using gamma-glutamyl-p-nitroanilide as a substrate and glycylglycine as an acceptor in 50 g/l of polyoxyethylene nonylphenol. Serum transpeptidase activity was assayed in 1080 healthy adults, the normal mean value being 14.8 mU/ml. The diagnostic evaluation of the enzyme in various hepatobiliary diseases is also discussed.
D000328:Adult; D001660:Biliary Tract Diseases / Q000175:diagnosis; D003902:Detergents / Q000494:pharmacology; D005260:Female; D006801:Humans; D008107:Liver Diseases / Q000175:diagnosis; D008297:Male; D008832:Microchemistry; D013053:Spectrophotometry / Q000379:methods; D005723:gamma-Glutamyltransferase / Q000097:blood* / Q000378:metabolism
D003902:Detergents; D005723:gamma-Glutamyltransferase