Protein and enzyme release from human leukocytes: influence of phenothiazine derivatives.
We studied the influence of chlorpromazine on the release of enzymes (beta-glucuronidase, EC 3.2.1.31; lactate dehydrogenase, EC 1.1.1.27; pyruvate kinase, 2.7.1.40) and proteins using human granulocytes isolated and maintained at 37 degrees C. Chlorpromazine had a biphasic effect on enzyme release and the inhibition of the glycolytic pathway could be demonstrated only at high concentrations of chlorpromazine, after one hour's incubation. The NAD+/NADH ratio was significantly perturbed at all the concentrations. This effect is time dependent. The action of 4 other phenothiazine derivatives made it possible to establish a relationship between their physico-chemical properties and protein release. The results are compared with those from other studies using other biological materials.
D014150:Antipsychotic Agents / Q000494:pharmacology*; D001798:Blood Proteins / Q000378:metabolism; D002746:Chlorpromazine / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005966:Glucuronidase / Q000097:blood*; D006098:Granulocytes / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000097:blood*; D007962:Leukocytes / Q000378:metabolism*; D011770:Pyruvate Kinase / Q000097:blood*; D013329:Structure-Activity Relationship
D014150:Antipsychotic Agents; D001798:Blood Proteins; D007770:L-Lactate Dehydrogenase; D011770:Pyruvate Kinase; D005966:Glucuronidase; D002746:Chlorpromazine