[Determination of the biological activity of heliomycin by a method of diffusion in agar].
A biological method for determination of geliomycin activity using agar diffusion is described. A nutrient medium containing Hottinger broth up to 35 mg per cent of amine nitrogen, 1.5 percent of agar-agar, tap water, pH 7.8 to 8.0 was used. Bacillus subtilis ATCC 6633 served as the test-culture. Geliomycin was dissolved in 0.1 N sodium hydroxide solution.
D000362:Agar; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000187:drug effects; D003624:Darkness; D004058:Diffusion; D004305:Dose-Response Relationship, Drug; D006863:Hydrogen-Ion Concentration; D007783:Lactones / Q000494:pharmacology; D008027:Light; D009824:Ointments; D011083:Polycyclic Compounds; D012996:Solutions; D013053:Spectrophotometry
D000900:Anti-Bacterial Agents; D007783:Lactones; D009824:Ointments; D011083:Polycyclic Compounds; D012996:Solutions; C007912:heliomycin; D000362:Agar
Opredelenie biologicheskoĭ aktivnosti geliomitsina metodom diffuzii v agar