In-vitro and in-vivo properties of canine blood mononuclear leukocytes separated by discontinuous albumin density gradient centrifugation.
Dogs were given 1 200 R whole body x-irradiation and transfused with frozen and thawed mononuclear leukocytes from a DLA identical, MLC negative donor dog. These leukocytes had been obtained from the peripheral blood by means of leukapheresis, using the IBM experimental cell separator after injection of 15 mg/kg body weight of dextran sulphate to increase the yield of CFUc upon collection. The segregation of leukocytes by means of the discontinuous albumin gradient centrifugation method resulted in a fraction 2 that contained a very high proportion of CFUc, and in other fractions 3 and 4 with a high proportion of lymphocytes with few CFUc. The dog receiving fraction-2 cells showed a rapid bone marrow recovery (permanent) and displayed no signs of gvh-reaction. The dogs receiving cells of fraction 3 or 4 died of gvh-reaction within 25 days. The dog receiving fraction 4 cells showed little hemopoietic recovery, but a marked lymph node hyperplasia of plasma cells.
D000818:Animals; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods; D002499:Centrifugation, Density Gradient / Q000379:methods; D004285:Dogs; D006087:Graft vs Host Reaction; D006098:Granulocytes / Q000166:cytology; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology; D007962:Leukocytes / Q000166:cytology / Q000276:immunology; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology