Characterization of sterol-ester hydrolase in Saccharomyces cerevisiae.
A homgenate of Saccharomyces cerevisiae grown under semi-anaerobic as well as aerobic conditions was found to catalyze the hydrolysis of fatty acid esters of sterols in the presence of Triton X-100. The enzyme levels in cells grown under various conditions were similar and the enzyme had a broad substrate specificity for sterol esters. The enzyme was localized in the mitochondrial fraction for the aerobically grown cells and in the mitochondrial and cytosolic fractions for the semi-anaerobically grown cells.
D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D010100:Oxygen; D011092:Polyethylene Glycols; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D002787:Sterol Esterase / Q000378:metabolism*; D013347:Subcellular Fractions / Q000201:enzymology; D013379:Substrate Specificity
D011092:Polyethylene Glycols; D002265:Carboxylic Ester Hydrolases; D002787:Sterol Esterase; D010100:Oxygen