Bovine adrenal tyrosine hydroxylase: purification and properties.
Bovine adrenal tyrosine hydroxylase has been obtained in a form that is 85 to 90% pure. Sodium dodecyl sulfate-gel electrophoresis and density gradient centrifugation studies have established that the subunit molecular weight of the chymotrypsin-solubilized enzyme is 34,000. The presence of iron in the purified enzyme (0.50 to 0.75 mol of iron/mol of enzyme) has been established. Crude particulate tyrosine hydroxylase can be activated by the phospholipid, phosphatidyl-L-serine, or by exposure to enzymatic phosphorylating conditions. Both forms of activation lower the Km of the enzyme for its 2-amino-4-hydroxypteridine cofactor. By contrast, tyrosine hydroxylase that has been solubilized by chymotrypsin cannot be activated by either of these methods.
D000313:Adrenal Medulla / Q000201:enzymology*; D000645:Ammonium Sulfate / Q000494:pharmacology; D000818:Animals; D001051:Apoenzymes / Q000302:isolation & purification; D002417:Cattle; D002499:Centrifugation, Density Gradient; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004589:Electrophoresis, Disc; D004789:Enzyme Activation; D006023:Glycoproteins / Q000032:analysis; D006493:Heparin / Q000494:pharmacology; D007501:Iron / Q000032:analysis; D007700:Kinetics; D008970:Molecular Weight; D010710:Phosphates / Q000378:metabolism; D010718:Phosphatidylserines / Q000494:pharmacology; D011622:Pterins / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism
D001051:Apoenzymes; D006023:Glycoproteins; D010710:Phosphates; D010718:Phosphatidylserines; D011622:Pterins; D006493:Heparin; D007501:Iron; D014446:Tyrosine 3-Monooxygenase; D000645:Ammonium Sulfate