Isolation of an Escherichia coli mutant deficient in thioredoxin reductase.
A mutant of Escherichia coli defective in thioredoxin reductase has been isolated and partially characterized. This mutant has no detectable thioredoxin reductase activity in vitro and yet it exhibits no in vivo defect in reduction of ribonucleotides. Evidence is presented that indicates that, in cells permeabilized via ether treatment, ribonucleoside diphosphate reduction can utilize glutathione as an alternate reducing system.
D004926:Escherichia coli / Q000201:enzymology* / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D008715:Methionine / Q000378:metabolism; D008717:Methionine Sulfoximine / Q000378:metabolism; D009154:Mutation; D009247:NADH, NADPH Oxidoreductases / Q000096:biosynthesis*; D009249:NADP / Q000378:metabolism; D012262:Ribonucleoside Diphosphate Reductase / Q000378:metabolism; D013696:Temperature; D013880:Thioredoxin-Disulfide Reductase / Q000096:biosynthesis* / Q000378:metabolism; D013879:Thioredoxins / Q000378:metabolism; D014500:Uracil Nucleotides / Q000378:metabolism
D014500:Uracil Nucleotides; D008717:Methionine Sulfoximine; D013879:Thioredoxins; D009249:NADP; D008715:Methionine; D012262:Ribonucleoside Diphosphate Reductase; D009247:NADH, NADPH Oxidoreductases; D013880:Thioredoxin-Disulfide Reductase; D005978:Glutathione