T antigen synthesis and resistance to interferon in human adenovirus type 12 infected chick cells.
Tye specific T antigen formation has been demonstrated in primary and secondary chick embryo cells (CEC) infected with adenovirus type 12. The frequency of cells synthesizing T antigen was closely dependent on the multiplicity of infection (MOI). At a MOI of 2.85 TCD50 per cell, T antigen was formed in 50% of cells. Cycloheximide inhibited T antigen formation while cytosine arabinoside had no such effect. CEC infected with adenovirus 12 produced interferon and T antigen, both appearing early and at about the same time of infection Exogenous chick interferon had no inhibitory effect on the formation of T antigen. In adenovirus 12-infected CEC, virus specific structural antigen could not be detected.
D000260:Adenoviruses, Human / Q000254:growth & development / Q000276:immunology*; D000818:Animals; D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D002460:Cell Line; D002642:Chick Embryo; D046508:Culture Techniques; D003513:Cycloheximide / Q000494:pharmacology; D003561:Cytarabine / Q000494:pharmacology; D007372:Interferons / Q000096:biosynthesis / Q000494:pharmacology*; D014357:Trypsin / Q000494:pharmacology
D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D003561:Cytarabine; D007372:Interferons; D003513:Cycloheximide; D014357:Trypsin