Evaluation of the counterimmunoelectrophoretic (CIE) procedure in a clinical laboratory setting.
The counterimmunoelectrophoretic (CIE) procedure was evaluated under clinical laboratory conditions to determine its validity and comparability with culture methods. The procedure was further evaluated to determine applicability to a variety of clinical samples. An inexpensive set-up was developed to utilize the CIE procedure at bench level. Results indicated the procedure to be sensitive in detecting Haemophilus influenzae, type b, and Neisseria meningitidis (meningococcus), group B. The procedure was more sensitive for detection of H. influenzae, type b, than for meningococcus, group B. The authors have confirmed the usefulness of the CIE procedure in the detection of group B streptococci, pneumococci and teichoic acid antibody to Staphylococcus aureus. Detection of Escherichia coli K 1 antigen was also accomplished by CIE. In the authors' laboratory the CIE procedure was superior to culture methods when used for the detection of H. influenzae, type b, and meningococcus group B.
D000818:Animals; D003378:Counterimmunoelectrophoresis / Q000379:methods*; D004926:Escherichia coli / Q000276:immunology; D006193:Haemophilus influenzae / Q000276:immunology; D006801:Humans; D007122:Immunoelectrophoresis / Q000379:methods*; D009345:Neisseria meningitidis / Q000276:immunology; D013211:Staphylococcus aureus / Q000276:immunology; D013292:Streptococcus agalactiae / Q000276:immunology; D013296:Streptococcus pneumoniae / Q000276:immunology