Hypoxia in fibroblast cultures. 3. 35S-sulfate incorporation into acid mucopolysaccharides as influenced by 5% O2 hypoxia with simultaneous changes in the lactate-and H-ion concentrations.
The influence of 5% O2 hypoxia on the 35S-sulfate incorporation into different mucopolysaccharide fractions was studied in monolayer cultures of embryonic rat fibroblasts before reaching the stationary phase. Besides the O2 concentration also the pH value and lactate concentration were varied in the experiments. The results are valid for proliferating cell cultures.
D000818:Animals; D002478:Cells, Cultured; D002809:Chondroitin Sulfates / Q000096:biosynthesis; D003871:Dermatan Sulfate / Q000096:biosynthesis; D005347:Fibroblasts / Q000378:metabolism* / Q000502:physiology; D006025:Glycosaminoglycans / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D000860:Hypoxia / Q000378:metabolism*; D066298:In Vitro Techniques; D007773:Lactates / Q000378:metabolism; D010101:Oxygen Consumption; D051381:Rats; D013431:Sulfates / Q000378:metabolism; D013462:Sulfur Radioisotopes
D006025:Glycosaminoglycans; D007773:Lactates; D013431:Sulfates; D013462:Sulfur Radioisotopes; D003871:Dermatan Sulfate; D002809:Chondroitin Sulfates