Consequences of ventromedial hypothalamic lesions on metabolism of perfused rat liver.
Metabolism of perfused livers from control and ventromedial hypothalamus (VMH)-lesioned rats has been studied. To eliminate the possibility that observed metabolic abnormalities could be realted to hyperphagia, VMH-lesioned rats were placed on restricted diet matching that of controls. Ten days postoperatively, VMH-lesioned rats had hyperinsulinemia, hypertriglyceridemia, increased blood urea nitrogen levels, together with decreased plasma free fatty acid (FFA) and glucose levels. Insulin release produced in vivo by a glucose load was much higher in VMH-lesioned than in control rats. Perfused livers from VMH-lesioned rats secreted more triglycerides and produced more urea than controls, whereas production of glucose and ketone bodies was reduced. Lipogenesis, newly synthesized triglyceride secretion, and the activity of acetyl-CoA carboxylase and fatty acid synthetase were greatest in livers from VMH-lesioned rats. Fasting abolished hyperinsulinemia and most of these observed metabolic alterations. After treatment with anti-insulin serum, the high rate of lipogenesis observed in livers from VMH-lesioned rats was restored toward normal. It is suggested that hyperinsulinemia may be partly responsible for the metabolic disorders observed in livers from nonhyperphagic VMH-lesioned rats.
D000103:Acetyl-CoA Carboxylase / Q000378:metabolism; D000818:Animals; D005215:Fasting; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D005502:Food; D005934:Glucagon / Q000097:blood; D005947:Glucose / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D007031:Hypothalamus / Q000503:physiopathology*; D007033:Hypothalamus, Middle / Q000503:physiopathology*; D007106:Immune Sera / Q000494:pharmacology; D007328:Insulin / Q000097:blood / Q000172:deficiency / Q000276:immunology; D007657:Ketone Bodies / Q000378:metabolism; D008055:Lipids / Q000096:biosynthesis; D008099:Liver / Q000378:metabolism*; D051381:Rats; D014280:Triglycerides / Q000097:blood; D014508:Urea / Q000378:metabolism
D005227:Fatty Acids; D007106:Immune Sera; D007328:Insulin; D007657:Ketone Bodies; D008055:Lipids; D014280:Triglycerides; D014508:Urea; D006003:Glycogen; D005934:Glucagon; D000103:Acetyl-CoA Carboxylase; D005947:Glucose