[Isoenzyme differentiation of gamma-glutamyltransferase by concanavalin A and Con- A-Sepharose (author's transl)].
In this investigation a new possibility of isoenzyme-differentiation of the gamma-glutamyl-transferase (GGT) (EC Nr.2.3.2.2.) was demonstrated by Concanavalin A and Con A-Sepharose. Because of the different sugar content of the glycoproteins distinction between liver- and kidney-GGT is possible. Furthermore it was possible for the first time to show a different precipitation behaviour of one glycoprotein to Concanavalin A in certain diseases. In cases of alcoholic hepatitis GGT looses its Concanavalin A-affinity because of increased neuraminic acid concentration. The possible reasons of the different behaviour to the binding affinity of Con A and Con A-Sepharose and GGT as well as additional use for enzyme-differentiation by Con A-Sepharose affinity chromatography are discussed.
D001379:Azathioprine / Q000494:pharmacology; D011232:Chemical Precipitation; D056486:Chemical and Drug Induced Liver Injury / Q000201:enzymology; D002846:Chromatography, Affinity; D003208:Concanavalin A; D000431:Ethanol; D006023:Glycoproteins / Q000032:analysis / Q000378:metabolism; D006801:Humans; D007527:Isoenzymes / Q000032:analysis*; D007674:Kidney Diseases / Q000201:enzymology; D008107:Liver Diseases / Q000201:enzymology; D009438:Neuraminic Acids / Q000378:metabolism; D009439:Neuraminidase; D011134:Polysaccharides; D012685:Sepharose; D005723:gamma-Glutamyltransferase / Q000032:analysis* / Q000097:blood / Q000652:urine
D006023:Glycoproteins; D007527:Isoenzymes; D009438:Neuraminic Acids; D011134:Polysaccharides; D003208:Concanavalin A; D000431:Ethanol; D012685:Sepharose; D005723:gamma-Glutamyltransferase; D009439:Neuraminidase; D001379:Azathioprine
Isoenzymdifferenzierung der Gamma-Glutamyltransferase mit Concanavalin A und Con A-Sepharose