Isocratic separation of some purine nucleotide, nucleoside, and base metabolites from biological extracts by high-performance liquid chromatography.
Separation of ATP, ADP, AMP, adenine, adenosine, cAMP, ITP, IDP, IMP, hypoxanthine, inosine, cIMP, the guanine series, NAD, NADPH, xanthine, 3-methylxanthine, theobromine, theophylline, and caffeine was accomplished using high-performance liquid chromatography with a microparticulate reversed-phase column. Under isocratic conditions all compounds could be eluted with reasonable resolution and retention time. Quantitation by peak height for several of the compounds was used to the 10-ng level.
D000227:Adenine Nucleotides / Q000032:analysis; D000818:Animals; D001792:Blood Platelets / Q000032:analysis; D002110:Caffeine / Q000032:analysis; D002415:Cats; D002851:Chromatography, High Pressure Liquid; D004912:Erythrocytes / Q000032:analysis; D006147:Guanine / Q000032:analysis; D007042:Hypoxanthines / Q000032:analysis; D007288:Inosine / Q000032:analysis; D007292:Inosine Nucleotides / Q000032:analysis; D009243:NAD / Q000032:analysis; D009249:NADP / Q000032:analysis; D009705:Nucleosides / Q000032:analysis*; D011685:Purine Nucleotides / Q000032:analysis*; D013154:Spleen / Q000032:analysis; D013805:Theobromine / Q000032:analysis
D000227:Adenine Nucleotides; D007042:Hypoxanthines; D007292:Inosine Nucleotides; D009705:Nucleosides; D011685:Purine Nucleotides; D009243:NAD; D002110:Caffeine; D009249:NADP; D007288:Inosine; D006147:Guanine; D013805:Theobromine