[Some properties of "soluble" Na+ and K+-ATPase obtained from various subcellular membrane structures of the brain by use of non-ionic detergents].
A comparative study was carried out of some properties of "soluble" Na+, K+-ATPase obtained from different subcellular membrane brain structures by means of non-ionic detergents of triton X-100 and digitonin. It is established that temperature and pH-optima of "soluble" Na+, K+-ATPase are close to these optima of the initial membrane preparations. A certain difference is observed in the dynamics of temperature and pH-dependence of Na+, K+-ATPase activity in the extracts from different subcellular structures. The stability of the preparations in storage was investigated. A conclusion is made that more stable enzyme extracts may be obtained by means of digitonin.
D000251:Adenosine Triphosphatases / Q000302:isolation & purification; D000818:Animals; D001921:Brain / Q000201:enzymology*; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism*; D004072:Digitonin; D004355:Drug Stability; D006863:Hydrogen-Ion Concentration; D008722:Methods; D011092:Polyethylene Glycols; D011188:Potassium; D012964:Sodium; D013696:Temperature
D011092:Polyethylene Glycols; D012964:Sodium; D000251:Adenosine Triphosphatases; D004072:Digitonin; D011188:Potassium
Deiaki vlastyvosti "rozchynnoï" Na+, K+-ATFazy, oderzhanoï z riznykh subklitynnykh membrannykh struktur mozku za dopomohoiu neĭonnykh deterhentiv