[Isolation and properties of the fibrinolytic enzyme from the Actinomyces thermovulgaris cultural broth].
A fibrinolytic enzyme was isolated from the cultural broth of Actinomyces thermovulgaris T-54 by precipitation with ammonium sulphate to 0.8 saturation and chromatography on GE-cellulose and CM-cellulose. The enzyme is homogeneous as was confirmed by disk electrophoresis in polyacrylamide gel. Some properties of the enzyme were studied. A modification of the method is suggested to assay the fibrinolytic activity.
D000190:Actinomyces / Q000201:enzymology*; D005337:Fibrin / Q000378:metabolism; D005342:Fibrinolysis; D006863:Hydrogen-Ion Concentration; D008722:Methods; D010447:Peptide Hydrolases / Q000302:isolation & purification / Q000378:metabolism; D013696:Temperature
D005337:Fibrin; D010447:Peptide Hydrolases
Vydelenie i svoĭstva fibrinoliticheskogo fermenta iz kul'tural'noĭ zhidkosti Actinomyces thermovulgaris