Complete enzymic digestion of acidic proteins.
Acidic proteins are usually resistant to complete enzymic hydrolysis. The increasing number of "unusual" amino acids, which are unstable to acid hydrolysis, makes it necessary to have a method of enzymic hydrolysis applicable to all proteins. The complete hydrolysis of four acidic proteins by subtilisin plus leucine amino-peptidase plus prolidase followed by carboxypeptidase C, is described. Recoveries of amino acids were in excellent agreement with the expected content from the known sequences.
D000596:Amino Acids / Q000032:analysis; D002268:Carboxypeptidases; D004150:Dipeptidases; D005340:Fibrinogen; D005755:Gastrins; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007931:Leucyl Aminopeptidase; D010435:Pepsinogens; D012260:Ribonucleases; D013381:Subtilisins
D000596:Amino Acids; D005755:Gastrins; D010435:Pepsinogens; D005340:Fibrinogen; D012260:Ribonucleases; D002268:Carboxypeptidases; D007931:Leucyl Aminopeptidase; D004150:Dipeptidases; C022449:proline dipeptidase; D013381:Subtilisins