Studies on urinary arylsulphatase activity in vitamin A deficient rats.
Urinary arylsulphatases (E.C.3.1.6.1) A and B were increased in male rats fasted for 24 hours. Excretion of non dialysable protein nitrogen decreased whereas creatinine excretion increased. On refeeding diet arylsulphatase A activity was restored to normal whereas arylsulphatase B was not normalised. A single oral supplementation of vitamin A acetate (20 000 IU) to rats fasted for 24 hours resulted in a significant reduction of both arylsulphatase A and B eventhough no further reduction of protein nitrogen excretion was evident. In vitamin A deficient male rats significant reduction in urinary excretion of both arylsulphatases A and B occured. In a smaller number of female rats depression of only arylsulphatase A was observed. This effect of vitamin A deficiency leading to reduced urinary arylsulphatase activity was evident even at the "weight plateau" stage when no reduction in food intake or growth had  occurred. These results suggest a possible direct or indirect role for vitamin A on urinary excretion pattern of arylsulphatases presumably released from lysosomes of tissues.
D000818:Animals; D001835:Body Weight; D002553:Cerebroside-Sulfatase / Q000652:urine*; D002803:Chondro-4-Sulfatase / Q000652:urine*; D003404:Creatinine / Q000652:urine; D005260:Female; D008297:Male; D009584:Nitrogen / Q000652:urine; D051381:Rats; D013217:Starvation / Q000201:enzymology; D013429:Sulfatases / Q000652:urine*; D014801:Vitamin A / Q000008:administration & dosage / Q000494:pharmacology; D014802:Vitamin A Deficiency / Q000201:enzymology*
D014801:Vitamin A; D003404:Creatinine; D013429:Sulfatases; D002553:Cerebroside-Sulfatase; D002803:Chondro-4-Sulfatase; D009584:Nitrogen