Antibodies to histones and histone-histone complexes: immunochemical evidence for secondary structure in histone 1.
Highly specific antibodies were raised to histone 1 (H 1) and the histone complexes H32-H42 AND H2A-H2B, isolated by salt extraction. Antibody to H1 could detect irreversible conformational changes in acid- or urea-treated H1. The antibodies showed different reactivities with chromosomes as compared to antibodies in acid-extracted histones and should be useful in studies of native chromatin and chromosome structure.
D000906:Antibodies; D000918:Antibody Specificity; D006657:Histones / Q000276:immunology / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D014508:Urea
D000906:Antibodies; D006657:Histones; D014508:Urea