Reduction of the graft-versus-host reactivity of mouse and rat spleen cells by 5alpha-androstane-3,17-dione.
The local graft-versus-host reaction as evaluated by the popliteal lymph node enlargement was used for studying the immunosuppressive potency of placental steroid 5alpha-androstane-3,17-dione. Spleen cells from virgin female mice and rats injected subcutaneously with this steroid compound produced in appropriate F1 recipients a significantly weaker reaction (P less than 0.001) than spleen cells from untreated or medium-treated control animals. On the other hand, the pretreatment of cell donors either with 5beta-androstane-3,17-dione or testosterone, the compounds which are not present in the mouse and rat placenta, did not influence the normal graft-versus-host reactivity.
D000731:Androstanes / Q000494:pharmacology*; D000818:Animals; D013196:Dihydrotestosterone / Q000494:pharmacology; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D006087:Graft vs Host Reaction / Q000187:drug effects*; D007165:Immunosuppression Therapy; D007664:Ketosteroids / Q000494:pharmacology; D008198:Lymph Nodes / Q000033:anatomy & histology; D051379:Mice; D008808:Mice, Inbred CBA; D009929:Organ Size; D010920:Placenta; D011247:Pregnancy; D051381:Rats; D013154:Spleen / Q000166:cytology* / Q000276:immunology
D000731:Androstanes; D007664:Ketosteroids; D013196:Dihydrotestosterone