A simple method to determine gammaglutamyl-cyclotransferase activity in erythrocytes.
A new method for the determination of erythrocyte gammaGCT is described. The method follows the release of alanine from gammaglutamyl alanine by a series of linked reactions that result in the oxidation of NADH to NAD. The pH optimum for human erythrocyte gammaGCT was found to be 9.0, and the Km for gammaglutamyl alanine was found to be 2.3 X 10(-3) M. Human erythrocytes had the highest activity of all the species studied (human, rabbit, dog, sheep, cattle, chicken).
D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D002645:Chickens; D004285:Dogs; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D008722:Methods; D011817:Rabbits; D012756:Sheep; D005723:gamma-Glutamyltransferase / Q000097:blood*
D005723:gamma-Glutamyltransferase