Iron-dependent binding of 8-anilinonaphthalene-1-sulphonate by both lactoferrin and transferrin.
Fluorescence of 8-anilinonaphthalene-1-sulphonate is enhanced by both lactoferrin and transferrin. The enhancement is relatively low between pH 4 and 8, and high at below pH 4. At physiological pH the fluorescence enhancement is higher with the iron-deprived than with the iron-saturated proteins. Binding of iron by lactoferrin is associated with lowering affinity for the dye.
D000817:Anilino Naphthalenesulfonates / Q000378:metabolism*; D005453:Fluorescence; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000378:metabolism; D007781:Lactoferrin / Q000378:metabolism*; D007782:Lactoglobulins / Q000378:metabolism*; D011485:Protein Binding; D012709:Serum Albumin; D014168:Transferrin / Q000378:metabolism*
D000817:Anilino Naphthalenesulfonates; D007782:Lactoglobulins; D012709:Serum Albumin; D014168:Transferrin; D007501:Iron; D007781:Lactoferrin