Cytochrome P-450 and alkane hydroxylase activity in Candida guilliermondii.
In the investigated Candida guilliermondii strain after growth on n-alkanes as the only carbon and energy source 5--10 nMol cytochrome P-450 per g cells (wet weight) could be detected. Cytochrome P-450 and alkane hydroxylase activity was found in the 100 000 xg pellet. Cofactor studies and inhibition experiments revealed the existence of a NADPH-dependent cytochrome P-450 alkane hydroxylase system.
D000473:Alkanes; D002175:Candida / Q000201:enzymology*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D003902:Detergents / Q000494:pharmacology; D005227:Fatty Acids; D007700:Kinetics; D006899:Mixed Function Oxygenases; D009249:NADP; D013053:Spectrophotometry
D000473:Alkanes; D003902:Detergents; D005227:Fatty Acids; D009249:NADP; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases