Impaired urinary acidification in the hypothyroid rat.
Since abnormalities in the renal handling of sodium and water in both the proximal and distal tubule have been described in primary hypothyroidism, this study was undertaken to examine renal tubular hydrogen secretion in this disorder. Metabolic acidosis was induced in hypothyroid rats (H) and their age matched controls (C) by the administration of an oral ammonium chloride load of 0.15 g/24 h/kg for three days. On day 3 animals were prepared for clearance and acid-base studies, receiving an infusion of Ringer's solution of 0.6 ml/hr/100 g during surgery and the experimental procedure. A 26% decrease in GFR (P less than 0.005) and a doubling in fractional excretion of sodium (P less than 0.02) were observed in H rats. The lowest blood pH and average bicarbonate concentration and the excretion of chloride were similar in the two groups, indicating that the acid load was reabsorbed and led to similar degrees of systemic acidification. Urine flow also was comparable in the two groups. Minimal urine pH after NH4Cl was 6.21 +/- 0.06 in H and 5.68 +/- 0.09 in C (P less than 0.001). Ammonium excretion was 28% (P less than 0.05) lower in H than in C. The defect in urine acidification in H was only partially corrected after 5 days on a low sodium diet and DOCA administration for 2 days. Fractional bicarbonate excretion at normal blood pH and bicarbonate concentration was not different in the two groups. These data indicate that hypothyroid rats have a mild defect in urine acidification and that it is localized predominantly in the distal tubule.
D000141:Acidosis, Renal Tubular / Q000503:physiopathology* / Q000652:urine; D000643:Ammonium Chloride / Q000008:administration & dosage / Q000652:urine; D000818:Animals; D001639:Bicarbonates / Q000378:metabolism; D004039:Diet, Sodium-Restricted; D004231:Diuresis; D005919:Glomerular Filtration Rate; D006863:Hydrogen-Ion Concentration; D007037:Hypothyroidism / Q000097:blood / Q000503:physiopathology / Q000652:urine*; D007686:Kidney Tubules, Distal / Q000503:physiopathology; D008297:Male; D009318:Natriuresis / Q000187:drug effects; D011188:Potassium / Q000652:urine; D051381:Rats; D012964:Sodium / Q000008:administration & dosage; D014556:Urine
D001639:Bicarbonates; D000643:Ammonium Chloride; D012964:Sodium; D011188:Potassium