Enhancement of NADPH-dependent lipid peroxidation activity by ethylene-diamineteraacetic acid (EDTA) in the presence of ferrous ion in rabbit liver microsomes.
The addition of EDTA to the incubation mixture containing rabbit liver microsomes and ferrous ion resulted in 2-fold increase of lipid peroxidation activity. Such an enhancement was not observed in rat liver microsomes. The maximum lipid peroxidation activity seen in rabbit microsomes in the presence of EDTA and ferrous ion was about 80% that seen in rat liver microsomes. From these results, it is likely that low lipid peroxidation activity in rabbit liver microsomes may account for the insufficiency of an EDTA-LIKE FACTORS(S) IN RABBIT LIVER MICROSOMES.
D000818:Animals; D004492:Edetic Acid / Q000494:pharmacology*; D005296:Ferrous Compounds / Q000494:pharmacology*; D066298:In Vitro Techniques; D007501:Iron / Q000494:pharmacology*; D050356:Lipid Metabolism; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction; D011817:Rabbits; D051381:Rats; D013268:Stimulation, Chemical
D005296:Ferrous Compounds; D009249:NADP; D004492:Edetic Acid; D007501:Iron