The metabolism of 3alpha, 7alpha, 12alpha-trihydroxy-5beta-cholestan-26-oic acid into cholic: an enzyme assay using homogenates of human liver.
An enzyme assay was developed to measure the conversion of the bile acid precursor, 3alpha, 7alpha, 12alpha-trihydroxy-5beta-cholestan-26-oic acid (THCA), into cholic acid using homogenates of human liver biopsies. The average rate of metabolism of THCA into cholic acid was found to be 3.9 +/- 0.5 (+/- 1 SD) pmoles of cholic acid formed/mg liver/minute in twelve normal liver biopsies. This assay system can be used to determine if the syndrome of neonatal cholestasis associated with a metabolic block in the conversion of THCA into cholic acid is transmitted as a genetic trait.
D000293:Adolescent; D000328:Adult; D000368:Aged; D001647:Bile Acids and Salts / Q000096:biosynthesis; D002776:Cholestanes / Q000378:metabolism*; D002793:Cholic Acids / Q000096:biosynthesis* / Q000302:isolation & purification; D003460:Crystallization; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008875:Middle Aged
D001647:Bile Acids and Salts; D002776:Cholestanes; D002793:Cholic Acids