A radioimmunoassay for the R-type binders of cobalamin.
A radioimmunoassay of R-type binders of cobalmin was devised and tested. The assay incorporated an antibody against purified human salivary R binder as the binding reagent. The labeled ligand was cyano[57Co]cobalamin bound to the R binder of pooled human saliva. The standard source of unlabeled ligand was also from human saliva of known R binder content. The assay was responsive to R binders of several sources, to either pure R or that of crude sources and equally to R binder saturated or unsaturated with cobalamin. It was not responsive to transcobalamin II. The assay was reproducible and reliable.
D000918:Antibody Specificity; D002352:Carrier Proteins / Q000097:blood / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008722:Methods; D011092:Polyethylene Glycols; D011863:Radioimmunoassay; D012471:Salivary Proteins and Peptides / Q000378:metabolism; D013696:Temperature; D013997:Time Factors; D014155:Transcobalamins / Q000378:metabolism; D014805:Vitamin B 12 / Q000378:metabolism*
D002352:Carrier Proteins; D012471:Salivary Proteins and Peptides; D014155:Transcobalamins; D011092:Polyethylene Glycols; D014805:Vitamin B 12