Ts mutant of NWS influenza virus: behaviour in vitro and in vivo.
After passaging the NWS influenza virus at increasing temperatures in the hamster embryo fibroblast (HEF) cell line, a temperature-resistant (tr) mutant which grew well at 39 degrees C was isolated. Attempts were made to isolate temperature-sensitive (ts) mutants from the tr virus. After growth in the presence of 5-fluorouracil, 233 virus clones were isolated. Among these only one was incapable of replicating at 39 degrees C. The physiological studies, the shift-up experiment and the electron microscopic investigation suggested that the genetic defect responsible for its ts character influenced a late function of the virus genome. In the lungs of intranasally infected mice, the ts mutant replicated to a lesser extent than the tr virus. During its growth in vivo, the ts virus exhibited a high degree of genetic stability.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D005472:Fluorouracil / Q000494:pharmacology; D006382:Hemadsorption; D006389:Hemagglutinins, Viral / Q000032:analysis; D007181:Inclusion Bodies, Viral; D008168:Lung / Q000382:microbiology; D051379:Mice; D009153:Mutagens; D009154:Mutation; D009975:Orthomyxoviridae / Q000254:growth & development* / Q000276:immunology; D013696:Temperature; D014764:Viral Proteins / Q000096:biosynthesis / Q000276:immunology; D014779:Virus Replication
D000956:Antigens, Viral; D006389:Hemagglutinins, Viral; D009153:Mutagens; D014764:Viral Proteins; D005472:Fluorouracil