Unusual behaviour of a protein: reversible quantitative precipitation of staphylococcal delta-haemolysin by low concentrations of some organic solvents.
Staphylococcal delta-haemolysin is precipitated from watery solutions by low concentrations of chloroform. The effect on this precipitation of pH, kind and concentration of salts, and concentration of, and time of exposure to, chloroform have been investigated. Some but not all of the other solvents tested cause a similar precipitation. The haemolytic activity is not destroyed and under appropriate conditions precipitation is quantitative. The dry lysin will take up nearly double its weight of chloroform when exposed to the vapour. Some is bound strongly.
D000645:Ammonium Sulfate; D001426:Bacterial Proteins / Q000302:isolation & purification*; D002122:Calcium Chloride; D011232:Chemical Precipitation; D002725:Chloroform; D006460:Hemolysin Proteins / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D008239:Lysine; D012965:Sodium Chloride; D013210:Staphylococcus / Q000032:analysis*; D013431:Sulfates; D013997:Time Factors; D014732:Vibration
D001426:Bacterial Proteins; D006460:Hemolysin Proteins; D013431:Sulfates; D012965:Sodium Chloride; D002725:Chloroform; D008239:Lysine; D002122:Calcium Chloride; D000645:Ammonium Sulfate