Boar seminal zinc-precipitable protein and the haemagglutinin.
About 30% of boar seminal plasma nitrogen is maximally precipitated at room temperature by 6 to 10 mM zinc in citrate solution at pH 8. A rise in the total nitrogen precipitated by 1 to 6 mM zinc is accompanied by a fall in the haemagglutinin titre of the supernatant fluid. At 6 mM zinc addition, 95% of the haemagglutinin is precipitated, but much of this is recoverable by re-solution of the zinc precipitate. Protein profile studies by gel-filtration chromatography of the zinc precipitate solution reveals a mixture of proteins, some of which are not by themselves zinc-precipitable.
D000373:Agglutinins / Q000032:analysis*; D000818:Animals; D002850:Chromatography, Gel; D006388:Hemagglutinins / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D008297:Male; D009584:Nitrogen / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D012661:Semen / Q000032:analysis* / Q000276:immunology; D013552:Swine; D015032:Zinc
D000373:Agglutinins; D006388:Hemagglutinins; D011506:Proteins; D015032:Zinc; D009584:Nitrogen