The role of the nucleus in the replication of measles virus.
The replication of measles and SSPE viruses in enucleate BSC-1 cells was measured by plaque assay, and the synthesis of virus-specific antigen was measured by indirect immunofluorescence. Titres of infectious virus produced in enucleate cells at 24 hours post inoculation (p.i.) were consistently 100-fold less, and the number of enucleate cells containing virus antigen at 30 hours p.i. was 10-fold less, than in nucleate control cultures. Enucleate cells at this time had 30% of the protein synthetic capacity of control nucleate cells, and supported the growth of Semliki Forest virus to titres equivalent to nucleate cell production.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002459:Cell Fusion; D002460:Cell Line; D002467:Cell Nucleus / Q000276:immunology / Q000382:microbiology*; D003584:Cytological Techniques; D003593:Cytoplasm / Q000276:immunology / Q000382:microbiology; D004668:Encephalitis Viruses / Q000254:growth & development / Q000276:immunology; D005455:Fluorescent Antibody Technique; D000882:Haplorhini; D008459:Measles virus / Q000254:growth & development* / Q000276:immunology; D013344:Subacute Sclerosing Panencephalitis / Q000382:microbiology; D014779:Virus Replication
D000956:Antigens, Viral