An improved technique for the preparation of chromosomes from cattle whole blood.
The method of preparation of chromosomes from cattle whole blood is described in detail and has been tested by use on more than 1000 different animal samples. It is shown that elevated levels of glutamine and 1-cystine increase the mitotic index and improve the keeping qualities of the medium 199. The recipe for whole medium is modified, describing the inclusion of heparin for the first time and recommending the exclusion of fetal calf serum. The optimum pH 7-3 is confirmed. The reported conditions of transport show that the best results pertain where air transport is used and the worst after prolonged journeys by car.
D000818:Animals; D001773:Blood Cells / Q000166:cytology; D001793:Blood Preservation; D002417:Cattle / Q000097:blood*; D002875:Chromosomes; D003470:Culture Media; D003553:Cystine / Q000494:pharmacology; D005973:Glutamine / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008722:Methods; D008938:Mitosis
D003470:Culture Media; D005973:Glutamine; D003553:Cystine