Mouse lymphocytes with and without surface immunoglobulin: preparative scale separation in polystyrene tissue culture dishes coated with specifically purified anti-immunoglobulin.
Mouse spleen cells could be preparatively separated into immunoglobulin positive (Ig+) and immunoglobulin-netative (Ig-)populations by incubating as many as 2 X 10(8) cells per 100 mm diameter petri plate coated with specifically purified goat anti-mouse immunoglobulin. The non-adherent population was 95% or more Ig-, and possessed graft versus host and cytotoxic effector activities, as would be expected for T cells. They could also give a mixed lymphocyte reaction and generate cytotoxic effector activity on culture in vitro. The adherent cells could not be released undamaged from plates coated with undiluted anti-Ig, but they could be released from plates coated with a 1/4 or 1/10 dilution of anti-Ig in an irrelevant antibody. The released cells were over 90% viable by trypan-blue staining, and 94% or more of the viable cells were Ig+.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000302:isolation & purification*; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D002448:Cell Adhesion; D002469:Cell Separation; D003601:Cytotoxicity Tests, Immunologic; D006041:Goats; D006087:Graft vs Host Reaction; D007106:Immune Sera / Q000494:pharmacology; D007111:Immunity, Cellular; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011137:Polystyrenes; D011947:Receptors, Antigen, B-Cell / Q000032:analysis*; D013154:Spleen / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D007106:Immune Sera; D011137:Polystyrenes; D011947:Receptors, Antigen, B-Cell