Tryptic- and chymotryptic-like proteinases in early and late preimplantation mouse blastocysts.
Neutral tryptic- and chymotryptic-like enzyme activities have been identified in extracts of early and late mouse blastocyts. The enzyme activities are distinguishable my means of: (a) reactivity with specific naphthylamide derivatives; (b) reactivity with tosyl-L-lysine-chloromethyl ketone or L-tosylamido-2-phenylethyl-chloromethyl ketone, respectively; (c) electrophoretic mobilities; and (d) specific activity profiles of late vs. early blastocysts.
D000818:Animals; D001587:Benzoylarginine-2-Naphthylamide; D001755:Blastocyst / Q000201:enzymology*; D002413:Cations, Divalent; D002918:Chymotrypsin; D047108:Embryonic Development; D010450:Endopeptidases; D005260:Female; D006863:Hydrogen-Ion Concentration; D051379:Mice; D011247:Pregnancy; D013997:Time Factors; D014107:Tosyllysine Chloromethyl Ketone; D014108:Tosylphenylalanyl Chloromethyl Ketone; D014357:Trypsin
D002413:Cations, Divalent; D014107:Tosyllysine Chloromethyl Ketone; D001587:Benzoylarginine-2-Naphthylamide; D014108:Tosylphenylalanyl Chloromethyl Ketone; D010450:Endopeptidases; D002918:Chymotrypsin; D014357:Trypsin