[Rheological properties of human bronchial secretions: demonstration of proline-rich polypeptides and their role (author's transl)].
Human bronchial secretions were examined for chemical components and rheological properties. Proline-rich polypeptides (PRP) obtained by ultrasonic treatment and by contact with a cationic resin (AG 50WX2) were purified by gel-filtration chromatography and high-voltage electrophoresis. The chemical composition of these components allowed a classification according to their proline, glycine, glutamic acid and lysine contents. Rheological experiments suggest a biological role for the PRP in the fibrillar structure of sputum.
D000596:Amino Acids / Q000032:analysis; D001980:Bronchi / Q000378:metabolism*; D002411:Cation Exchange Resins; D055598:Chemical Phenomena; D002621:Chemistry; D002850:Chromatography, Gel; D004590:Electrophoresis, Paper; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010455:Peptides / Q000032:analysis* / Q000302:isolation & purification; D011392:Proline / Q000032:analysis*; D012212:Rheology; D014465:Ultrasonics; D014783:Viscosity
D000596:Amino Acids; D002411:Cation Exchange Resins; D010455:Peptides; D011392:Proline
Proprieties rheologiques des secretions bronchiques: mise en evidence et role de polypeptides riches en proline (PRP)