[Properties of alpha-chymotrypsin and cis-cinnamoylchymotrypsin in free, covalently bound to a carrier and microcapsulated states].
alpha-Chymotrypsin and its light-sensitive derivative, cis-cinnamoylchymotrypsin, free and immobilysed (by means of microcapsulation, covalent binding with sepharose 4B and p-aminobenzyl cellulose) are studied. Effect of pH on the hydrolysis rate of N-alpha-acetyl-L-tyrosine ethyl ester by different immobilized enzyme preparations, their acylation with isomeric mixture of cis- and trans-cinnamoylimidazole, reactivation of stable cis-derivatives of different enzyme samples and thermal stability of native and microcapsulated enzyme preparations are investigated.
D001665:Binding Sites; D002384:Catalysis; D002918:Chymotrypsin; D002934:Cinnamates; D004800:Enzymes, Immobilized; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008027:Light
D002934:Cinnamates; D004800:Enzymes, Immobilized; D002918:Chymotrypsin; C019903:cis-cinnamoylchymotrypsin
Svoĭstva al'fa-khimotripsina i tsis-tsinnamoilkhimotripsina v svobodnom, kovalentno sviazannom s nositelem i mikrokapsulirovannom sostoianii