[A sensitive fluorometric determination of catechol methyltransferase activity (author's transl)].
A method for the determination of catechol-methyltransferase activity is described, based on the measurement of fluorometric intensity of 7-hydroxy-6-methoxycoumarin (scopoletin), enzymatically produced by dihydroxycoumarin in the presence of the methyl donor S-adenosylmethionine.
D000818:Animals; D002394:Catechol O-Methyltransferase / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D003374:Coumarins; D006719:Homovanillic Acid / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D008722:Methods; D051381:Rats; D012436:S-Adenosylmethionine; D012995:Solubility; D013050:Spectrometry, Fluorescence
D003374:Coumarins; D012436:S-Adenosylmethionine; D002394:Catechol O-Methyltransferase; D006719:Homovanillic Acid
Eine empfindliche fluorometrische Bestimmungsmethode für die Catechol-Methyltransferase