T-lymphocyte heterogeneity in the rat: separation of functional subpopulations using a monoclonal antibody.
W3/25 antibody is the monoclonal product of a hybrid cell resulting from the fusion of a mouse myeloma cell line with spleen cells from a mouse immunized with rat thymocytes. Pure clones have been derived, and segregants free of parental myeloma chains have been isolated. Previous studies have shown that this antibody recognizes a subpopulation of T cells among rat thoracic duct lymphocytes. In the work reported here, three T-cell functions were assayed after separating rat thoracic duct lymphocytes on the fluorescence-activated cell sorter on the basis of labeling with W3/25 antibody. Two of the functional activities appeared to be completely segregated by this procedure. Thus, helper cell activity for an anti-hapten plaque-forming cell response was confined to the labeled population, whereas the allogeneic suppressive effect produced in a parental vector F1 adoptive transfer was mediated by cells in the unlabeled fraction. The third function, graft-versus-host activity, was almost entirely contained within the labeled subpopulation. It is concluded that the antigenic determinant recognized by the monoclonal antibody W3/25 is a differentiation marker for T-cell functional subpopulations.
D000818:Animals; D000918:Antibody Specificity; D000954:Antigens, Surface / Q000032:analysis; D002999:Clone Cells / Q000276:immunology; D006087:Graft vs Host Reaction; D007111:Immunity, Cellular; D007158:Immunologic Techniques; D007165:Immunosuppression Therapy; D007519:Isoantigens / Q000032:analysis; D008211:Lymphocyte Cooperation; D051381:Rats / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D000954:Antigens, Surface; D007519:Isoantigens