Induction of autolysis in Bacillus subtilis by ochratoxin A.
Ochratoxin A (OTA) added during the exponential growth phase at a concentration higher than 12 microgram/ml caused autolysis of Bacillus subtilis. Optical density of cultures decreased, and at higher concentrations the cultures became sterile. Optimum OTA-induced lysis was about pH 5. At concentrations below 10 microgram/ml, protein synthesis was inhibited more strongly than RNA synthesis. Cell wall synthesis was also strongly inhibited. A fraction extracted from the lysates had the property of a lysis inhibitor. The relevance of this fraction in respect to autolysis is discussed.
D001412:Bacillus subtilis / Q000187:drug effects* / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis; D001433:Bacteriolysis; D002473:Cell Wall / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D009793:Ochratoxins / Q000494:pharmacology*; D012329:RNA, Bacterial / Q000096:biosynthesis
D001426:Bacterial Proteins; D009793:Ochratoxins; D012329:RNA, Bacterial