Induction of placental alkaline phosphatase in choriocarcinoma cells by 5-bromo-2'-deoxyuridine.
Growth of choriocarcinoma cells in the presence of 5-bromo-2'-deoxyuridine (BrdUrd) results in a 30- to 40-fold increase in alkaline phosphatase activity. The effects of BrdUrd is specific for phosphatase with an alkaline pH optimum. The induction by BrdUrd is probably not due to the production of an altered enzyme, since the induced enzyme resembles the basal enzyme in thermal denaturation and kinetic properties. Enzyme induction can be prevented by thymidine but not by deoxycytidine or deoxyuridine. The induction of alkaline phosphatase appears to require incorporation of the BrdUrd into cellular DNA. The presence of BrdUrd in the growth medium is not necessary for alkaline phosphatase induction in proliferating cells containing BrdUrd-substituted genomes. However, enzyme induction and maintenance of the induced levels of alkaline phosphatase in nonproliferating cells containing BrdUrd-substituted DNA requires the presence of the analogues in the medium. The induction of alkaline phosphatase by BrdUrd in probably an indirect process.
D000469:Alkaline Phosphatase / Q000096:biosynthesis* / Q000378:metabolism; D001973:Bromodeoxyuridine / Q000494:pharmacology*; D002460:Cell Line; D002822:Choriocarcinoma; D002999:Clone Cells; D003513:Cycloheximide / Q000494:pharmacology; D004261:DNA Replication; D004273:DNA, Neoplasm / Q000096:biosynthesis; D003609:Dactinomycin / Q000494:pharmacology; D003839:Deoxyadenosines / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009363:Neoplasm Proteins / Q000096:biosynthesis; D011247:Pregnancy; D011691:Puromycin / Q000494:pharmacology; D012334:RNA, Neoplasm / Q000096:biosynthesis; D013937:Thymidine Kinase / Q000378:metabolism
D004273:DNA, Neoplasm; D003839:Deoxyadenosines; D009363:Neoplasm Proteins; D012334:RNA, Neoplasm; D003609:Dactinomycin; D011691:Puromycin; D003513:Cycloheximide; D013937:Thymidine Kinase; D000469:Alkaline Phosphatase; D001973:Bromodeoxyuridine