Acid mucopolysaccharides in fibroblast cultures. 2. 35S-sulfate incorporation kinetics in dependence on pH-value and cell density.
Cultures of embryonic rat fibroblasts were incubated with 35S-sulfate at pH 6.6 and 7.4 (Eagle basal medium plus HEPES buffer) for 12 to 48 hours. The acid mucopolysaccharides were isolated and fractionated after the method of SVEJCAR and ROBERTSON. Sulfate incorporation was determined by liquid scintillation counting.
D000818:Animals; D002478:Cells, Cultured; D005591:Chemical Fractionation; D002809:Chondroitin Sulfates / Q000378:metabolism; D003871:Dermatan Sulfate / Q000378:metabolism; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000378:metabolism*; D006025:Glycosaminoglycans / Q000096:biosynthesis / Q000378:metabolism*; D006497:Heparan Sulfate / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D051381:Rats; D013431:Sulfates / Q000378:metabolism
D002809:Chondroitin Sulfates; D003871:Dermatan Sulfate; D006025:Glycosaminoglycans; D006497:Heparan Sulfate; D013431:Sulfates