An alkyl etherase in rat liver.
1. Diethyl ether, which is known to be partly metabolized in vivo, has been found to show an O2 uptake with the rat liver microsomal membranes; a similar reaction is given with other short chain aliphatic ethers, isoproply and n-butyl ether. 2. The "etherase" reaction is optimal at pH 7.2-7.4 and is not accompanied by an increased formation of malondialdehyde. 3. When CoA is added to the microsomes together with a source of oxaloacetate and the consensing enzyme synthase, the etherase present forms citrate from diethyl ether, indicating an acetylation of CoA, which then enters the citric acid cycle. 4. Similarly to 3, fluorocitrate is formed from methyl fluoroethyl ether. 5. Differing from plasmalogens, a tetrahydropteridine does not have to be added as a co-factor.
D000107:Acetylation; D000818:Animals; D002951:Citrates / Q000096:biosynthesis; D003065:Coenzyme A / Q000494:pharmacology; D004986:Ether / Q000378:metabolism*; D004987:Ethers / Q000378:metabolism; D005019:Ethyl Ethers / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D010101:Oxygen Consumption / Q000187:drug effects; D011621:Pteridines / Q000494:pharmacology; D051381:Rats
D002951:Citrates; D004987:Ethers; D005019:Ethyl Ethers; D011621:Pteridines; D004986:Ether; D003065:Coenzyme A