Glycolipid glycosyl transferases of a hamster cell line in culture. I. Kinetic constants, substrate and donor nucleotide sugar specificities.
The properties of enzymes catalysing the transfer of a galactose from UDP-galactose to exogenous ceramide monohexoside and ceramide di-hexoside derived from the Syrian hamster cell line NIL 2 were studied. The products of these enzymes were characterized by chemical and enzymatic methods. Kinetic analyses showed that the enzymes are susceptible to inhibition and activation by a number of substrate analogues. The kinetic and inhibition constants, glycolipid substrate specificity and nucleotide sugar donor specificity have been studied.
D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002518:Ceramides; D002554:Cerebrosides / Q000378:metabolism*; D005700:Galactosyltransferases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D013329:Structure-Activity Relationship; D013696:Temperature; D014531:Uridine Diphosphate Galactose / Q000378:metabolism
D002518:Ceramides; D002554:Cerebrosides; D014531:Uridine Diphosphate Galactose; D005700:Galactosyltransferases