Fluorescence polarization studies on the binding between glutamate dehydrogenase and cytoplasmic aspartate aminotransferase.
Polarization of fluorescence measurements on aspartate aminotransferase (from pig heart cytosol) labeled with fluorescein isothiocyanate have been used to detect the formation of a soluble complex of this protein wich glutamate dehydrogenase from bovine liver. The binding of the labeled transaminase to dehydrogenase is detectable at catalytic concentrations of the enzymes.
D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D001665:Binding Sites; D002417:Cattle; D003600:Cytosol / Q000201:enzymology; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D009206:Myocardium / Q000201:enzymology; D011485:Protein Binding; D012710:Serum Albumin, Bovine; D013050:Spectrometry, Fluorescence; D013552:Swine
D012710:Serum Albumin, Bovine; D005969:Glutamate Dehydrogenase; D001219:Aspartate Aminotransferases