Further studies of the physical and metabolic properties of foot-and-mouth disease virus temperature-sensitive mutants.
Three temperature-sensitive (ts) mutants of foot-and-mouth disease virus were classified as ribonucleic acid negative and as belonging to the same complementation group when measured by virus yields and [3H] uridine incorporation in paired, mixed infections at the nonpermissive temperature (38.5C). Mutant ts-22, the only mutant able to produce plaques at 38.5 C, was more sensitive to acid than were the parental wild-type or other mutant viruses. Diethylaminoethyl-dextran did not enhance the plaque-forming ability of the mutant viruses at 38.5C. All of the viruses inhibited host cell protein syntehsis at both permissive (33C) and nonpermissive (38.5C) temperatures.
D005537:Aphthovirus / Q000254:growth & development / Q000378:metabolism*; D003637:DEAE-Dextran / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D009154:Mutation; D012367:RNA, Viral / Q000096:biosynthesis; D013696:Temperature; D010948:Viral Plaque Assay
D012367:RNA, Viral; D003637:DEAE-Dextran