[Preparation and properties of beta-galactosidase linked covalently with KM-cellulose].
Fungal beta-galactosidase was immobilized by covalent binding with KM-cellulose. The resultant preparation contained 3 mg protein per 1 g carrier; its specific activity was 65% of the initial one. As a result of immobilization pH optimum remained unchanged whereas the temperature optimum decreased from 65 degrees to 50 degrees. The seemings Km of the immobilized enzyme varied insignificantly as compared with Km of the soluble enzyme.
D001665:Binding Sites; D002846:Chromatography, Affinity; D005658:Fungi / Q000201:enzymology; D005696:Galactosidases / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008433:Mathematics; D011485:Protein Binding; D012995:Solubility; D013696:Temperature
D005696:Galactosidases
Poluchenie i svoĭstva beta-galaktozidazy, kovalentno sviazannoĭ c KM-tselliulozoĭ