The effect of ion-exchange column chromatography on separation of X and Y chromosome-bearing human spermatozoa.
Separation of X and Y chromosome-bearing spermatozoa has been attempted using ion-exchange column chromatography, with cation- and anion-exchange resins of low, intermediate, and high ionic strength. Examination of F-bodies on the Y chromosome of treated human sperm and progeny resulting from insemination of treated rabbit spermatozoa indicates that in none of the cases investigated did the treatment cause a separation of X and Y chromosome-bearing spermatozoa. The treatment does appear to filter out dead rabbit (and bull) spermatozoa, but the possible beneficial effects of this phenomenon are as yet uninvestigated.
D000818:Animals; D002417:Cattle; D002470:Cell Survival; D002852:Chromatography, Ion Exchange; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D011817:Rabbits; D012730:Sex Chromosomes; D012743:Sex Preselection / Q000379:methods; D013094:Spermatozoa / Q000648:ultrastructure*