Determination of clomipramine and desmethyl-clomipramine in plasma or urine by the double-radioisotope derivative technique.
A double radioisotope derivative method was developed for the determination of clomipramine and desmethyl-clomipramine in plasma or urine. After addition of 14C-labeled clomipramine and desmethyl-clomipramine as internal standards and extractive isolation of both compounds, desmethyl-clomipramine is acetylated with [3H]acetic anhydride. The [3H]acetamide is separated from clomipramine by thin-layer chromatography and its radioactivity is measured. Clomipramine, extracted from the cilica gel, is reacted with trichloroethyl chloroformate. The urethane is saponified and decarboxylated. The resulting desmethyl-clomipramine is acetylated with [3H]acetic anhydride. The [3H]acetamide is purified by thin-layer chromatography and its radioactivity is measured. The sensitivity of the method is 15 mug/liter for clomipramine and 2 mug/liter for desmethyl-clomipramine. Its specificity was made sure by a cross-check with a gas chromatography-mass spectrometry technique.
D002250:Carbon Radioisotopes; D002997:Clomipramine / Q000031:analogs & derivatives / Q000032:analysis* / Q000097:blood / Q000652:urine; D003984:Dibenzazepines / Q000032:analysis*; D005069:Evaluation Studies as Topic; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007553:Isotope Labeling; D008722:Methods; D014316:Tritium
D002250:Carbon Radioisotopes; D003984:Dibenzazepines; D014316:Tritium; D002997:Clomipramine