Defined conditions for synthesis of Bacillus cereus enterotoxin by fermenter-grown cultures.
A strain of Bacillus cereus produced high levels of enterotoxin when grown in a semidefined medium in a laboratory scale fermenter. The optimum conditions for enterotoxin synthesis by cultures grown in this medium, which contained Casamino Acids and yeast extract, were found to be: inoculation of vigorously gorwing culture at the 1% level, addition of glucose at a concentration of 1%, control of culture pH at 8.0, incubation at 32 degrees C, use of a moderate stirring rate, and addition of air at low flow rates to minimize foaming. The enterotoxin yield in fermenter-grown cultures was approximately 20 to 50 times higher than the yield obtained in shake flask cultures.
D001409:Bacillus cereus / Q000378:metabolism*; D003470:Culture Media; D004768:Enterotoxins / Q000096:biosynthesis*; D005285:Fermentation; D005506:Food Contamination; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D010100:Oxygen; D013696:Temperature
D003470:Culture Media; D004768:Enterotoxins; D005947:Glucose; D010100:Oxygen