Specific, water-soluble polypeptides in identified neurons of Aplysia californica.
Application of an ethylene glycol lysis technique to extract water-soluble, low molecular weight polypeptides in Aplysia neurons, was used in conjunction with microgradient gel electrophoresis and micro-isoelectric focusing, to identify unique polypeptides in specific, identified neurons. The polypeptides found in neurons R15, R3-13, R14, and the bag cells were particularly abundant, consistent with the previously suggested neurosecretory role for these cells. Water extraction of the strongly basic polypeptides (pI 10.7) in R3-13 and R14 required an acidic lysis medium.
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D005026:Ethylene Glycols; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D008970:Molecular Weight; D008974:Mollusca / Q000032:analysis*; D009474:Neurons / Q000032:analysis*; D010455:Peptides / Q000032:analysis; D012995:Solubility
D005026:Ethylene Glycols; D010455:Peptides