Determination of free and esterified cholesterol by a kinetic method. I. The introduction of the enzymatic method with 2,2'-azino-di-3[ethyl-benzthiazolin sulfonic acid (6)] (ABTS).
A new, simple kinetic method is described for the determination of serum cholesterol based on the oxidation of 2,2'-azino-di-3[ethyl-benzthiazolin sulfonic acid (6)] (ABTS) by use of a single aqueous reagent. This assay procedure is rapid, specific, reproducible and applicable to the measurement of free and esterified cholesterol in a continuous procedure. The method requires no prior treatment of sample and linear kinetics are obtained up to 13 mmol/l cholesterol. The use of ABTS permits the direct calculation of cholesterol concentrations from absorbance changes at 410 nm (A = epsilon - c - d).
D052160:Benzothiazoles; D002784:Cholesterol / Q000097:blood*; D002788:Cholesterol Esters / Q000097:blood*; D002789:Cholesterol Oxidase; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008722:Methods; D010544:Peroxidases; D002787:Sterol Esterase / Q000097:blood; D013451:Sulfonic Acids; D013656:Taurocholic Acid / Q000494:pharmacology; D013844:Thiazoles
D052160:Benzothiazoles; D002788:Cholesterol Esters; D013451:Sulfonic Acids; D013844:Thiazoles; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D013656:Taurocholic Acid; D002784:Cholesterol; D002789:Cholesterol Oxidase; D010544:Peroxidases; D002787:Sterol Esterase