Some properties of acetylcholinesterase from rat retina.
Acetylcholinesterase (AChE, EC 3.1.1.7) of rat retina was studied with respect to its kinetic and other properties, and a comparison was made with the enzyme from brain. The subcellular distribution of the retinal AChE showed that the enzyme was concentrated in the synaptosomal-mitochondrial fraction although in the brain the AChE was distributed more evenly between the fractions studied. The enzyme from both retina and brain was easily solubilised and exhibited a Km of the order of 10(-4) M. The pH optimum was 8.3-8.6 for the AChE from both tissues for both the soluble and particulate enzyme.
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000201:enzymology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008297:Male; D009928:Organ Specificity; D051381:Rats; D012160:Retina / Q000201:enzymology*; D013347:Subcellular Fractions / Q000201:enzymology
D000110:Acetylcholinesterase