Studies on adrenaline-induced lipolysis in artificial lipid micelles.
Lipid micelles were prepared by incubating a mixture of glycerides (triolein, diolein, and monoolein), and lecithin in Krebs-Ringer phosphate buffer at 37 degrees C for 30 min. It was found that adrenaline stimulated the release of free fatty acids in a lipolytic system consisting of the lipid micelles and adipose tissue lipase. Adrenaline did not increase the cyclic AMP content of the reaction mixture. Dibutyryl cyclic AMP, theophylline, and phospholipase C increased the rate of lipolysis in the system but cyclic AMP and phospholipase D did not.
D003994:Bucladesine; D003102:Colloids; D000242:Cyclic AMP; D004075:Diglycerides; D004837:Epinephrine; D005230:Fatty Acids, Nonesterified; D005989:Glycerides; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008049:Lipase / Q000378:metabolism; D008823:Micelles; D010713:Phosphatidylcholines; D010740:Phospholipases / Q000378:metabolism; D013806:Theophylline; D014304:Triolein
D003102:Colloids; D004075:Diglycerides; D005230:Fatty Acids, Nonesterified; D005989:Glycerides; D008823:Micelles; D010713:Phosphatidylcholines; D014304:Triolein; D003994:Bucladesine; D013806:Theophylline; D000242:Cyclic AMP; D010740:Phospholipases; D008049:Lipase; D004837:Epinephrine