Media for identification of Gibberella zeae and production of F-2-(Zearalenone).
Media are described for the isolaton of Fusarium graminearum in the perithecial state, Gibberella zeae, and for the production of F-2 (zearalenone) by Fusarium species. On soil extract-corn meal agar isolated medium, G. Zeae produced perithecia in 9 to 14 days under a 12-h photoperiod. Species of Fusarium were screened for F-2 production on a liquid medium. From strains that produced F-2, the yields, from stationary cultures of G. zeae and F. culmorum after 12 days of incubation, ranged from 22 to 86 mg/liter. Three strains produced no F-2. Glumatic acid, starch, yeast extract,and the proper ratio of medium volume-to-flask volume were necessary for F-2 synthesis.
D003470:Culture Media; D002523:Edible Grain; D005069:Evaluation Studies as Topic; D005670:Fusarium / Q000302:isolation & purification / Q000378:metabolism; D005874:Gibberella / Q000302:isolation & purification / Q000378:metabolism*; D005971:Glutamates / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006999:Hypocreales / Q000378:metabolism*; D012118:Resorcinols / Q000096:biosynthesis*; D012441:Saccharomyces cerevisiae; D012988:Soil Microbiology; D013213:Starch / Q000378:metabolism; D003313:Zea mays; D015025:Zearalenone / Q000096:biosynthesis*
D003470:Culture Media; D005971:Glutamates; D012118:Resorcinols; D015025:Zearalenone; D013213:Starch