Effects of dietary vitamin B-2 and vitamin E on the delta9-desaturase and catalase activities in the rat liver microsomes.
The effects of dietary vitamin B-2 and vitamin E on delta9-desaturation  of stearoyl-CoA, catalase, glutathione peroxidase, superoxide dismutase and electron transport components in rat liver microsomes have been investigated. delta9-desaturase activities were decreased on diets deficient of vitamin B-2, E and supplemented with E. Among the peroxide-scavenging enzymes, only the catalase activity in microsomes correlates significantly with delta9-desaturase activity. In vitro addition of bovine catalase had no effect on microsomal delta9-desaturase activity on control diet. However, it enhanced the delta9-desaturation in microsomes on vitamin B-2-deficient diet which contained low catalase and high superoxide dismutase activities, compared to those in microsomes of control diet. It is suggested that the hydrogen peroxide-generating and -decomposing systems may play an important role on the delta9-desaturase activity in microsomes.
D000818:Animals; D002374:Catalase / Q000378:metabolism*; D044943:Fatty Acid Desaturases / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000378:metabolism; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology*; D051381:Rats; D012256:Riboflavin / Q000494:pharmacology*; D013230:Stearoyl-CoA Desaturase / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism; D014810:Vitamin E / Q000494:pharmacology*
D014810:Vitamin E; D002374:Catalase; D005979:Glutathione Peroxidase; D044943:Fatty Acid Desaturases; D013230:Stearoyl-CoA Desaturase; D013482:Superoxide Dismutase; D012256:Riboflavin