Inhibition of Newcastle disease virus replication by 6-azauridine. I. Inefficacy of purified uridine kinase, effect of adenosine-5'-triphosphate.
As distinct from cell-free extracts prepared from tumour cells, partially purified uridine kinase prepared from the same cells was not effective in 6-azauridine (AzUrd) inhibition of Newcastle disease virus (NDV) replication. This showed that uridine kinase was not the effective component of cell-free extracts. Adenosine-5'-triphosphate (ATP) was found to exert a synergic effect in combination with AzUrd in the inhibition of NDV replication.
D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D001380:Azauridine / Q000494:pharmacology*; D002286:Carcinoma, Ehrlich Tumor / Q000201:enzymology; D002474:Cell-Free System; D002642:Chick Embryo; D046508:Culture Techniques; D004357:Drug Synergism; D051379:Mice; D009522:Newcastle disease virus / Q000254:growth & development*; D010770:Phosphotransferases / Q000494:pharmacology*; D014541:Uridine Kinase / Q000378:metabolism / Q000494:pharmacology*; D014779:Virus Replication / Q000187:drug effects*
D001380:Azauridine; D000255:Adenosine Triphosphate; D010770:Phosphotransferases; D014541:Uridine Kinase