Fluorometric assay for N-acetylprocainamide.
We describe a simple, rapid fluorometric assay for separate quantitative analysis of procainamide and N-acetylprocainamide in mixtures. The effective lenear range (fluorescence vs. concentration) in serum is 0.1 to 10.0 mg/liter, regardless of the ratio (by weight) of the two drugs from 1:10 to 10:1. Analytical recoveries by the extraction method used were 100.0 +/- 3.0% and 98.0 +/- 4.0%, respectively. For determination of either compound, the maximum coefficient of variation was 10%.
D006801:Humans; D006863:Hydrogen-Ion Concentration; D011342:Procainamide / Q000031:analogs & derivatives* / Q000097:blood; D013050:Spectrometry, Fluorescence
D011342:Procainamide