A new endoribonuclease from Escherichia coli. Ribonuclease N.
A new ribonuclease called RNase N was isolated from Escherichia coli. It is a nonspecific endoribonuclease that can cleave rRNA, poly(U), and poly(C) to small oligonucleotides and 5'-mononucleotides. It requires monovalent cations and is inhibited by divalent cations. It is suggested that this enzyme plays a role in the decay of rRNA,under various starvation conditions and perhaps in the decay of mRNA.
D002414:Cations, Monovalent / Q000494:pharmacology; D004720:Endonucleases / Q000302:isolation & purification / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D012260:Ribonucleases / Q000302:isolation & purification / Q000378:metabolism*; D012270:Ribosomes / Q000201:enzymology
D002414:Cations, Monovalent; D008345:Manganese; D004720:Endonucleases; D012260:Ribonucleases; D008274:Magnesium