[Dynamics of production and various properties of interferon-like inhibitors formed under the influence of AET and cystaphos in vivo and in cell cultures].
Interferon-like virus inhibitors appeared in the blood serum of animals and in the culture fluid 15 min.--1 hour and 4-6 hours after administration of AET (S, beta-amino-ethylisotiuronium) and cystaphos (monosodium salt of beta-aminoethylthiophosphorus acid). These inhibitors showed species-specificity, were inactivated by trypsin and by heating at 37 degrees C for 2 hours and at 56 degrees C for 1 hour, did not sediment at 100,000 g. It is assumed that these are two different inhibitors of which the one forming the early peak pre-exists in the cell. It is probably associated with cell membranes and is released as a result of chemical effect of aminothyols. The late peak of inhibitor is synthesized de novo as inducated by its sensitivity to the effect of puromycin and cycloheximide.
D000818:Animals; D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology; D003539:Cystaphos / Q000494:pharmacology*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007369:Interferon Inducers; D007372:Interferons / Q000032:analysis / Q000096:biosynthesis* / Q000097:blood; D051379:Mice; D009946:Organothiophosphorus Compounds / Q000494:pharmacology*; D011691:Puromycin / Q000494:pharmacology; D014357:Trypsin
D007369:Interferon Inducers; D009946:Organothiophosphorus Compounds; D011691:Puromycin; D007372:Interferons; D003513:Cycloheximide; D014357:Trypsin; D003539:Cystaphos
Dinamika vyrabotki i nekotorye svoistva interferonopodobnykh ingibitorov, obrazuiushchikhsia pod vliianiem AET i tsistafosa v organizme i kletochnykh kul'turakh