Effect of the pH of culture medium on the alkalophilicity of a species of Bacillus.
The amino acid incorporation and alpha-amino-isobutyric acid (AIB) uptake of an alkalophilic Bacillus grown at pH 8.2 (the pH 8-bacteria) were much less pH dependent (less alkalophilic) than those of the organisms grown at pH 10.0 (the pH 10-bacteria), respectively. The rate of AIB uptake of the pH 10-bacteria was almost the same as that of the pH 8-bacteria, while the rate of amino acid incorporation of the pH 10-bacteria was higher than that of the pH 8-bacteria in alkaline environments. The colloidal titration with clupein showed that the amount of negative charge on the pH 10-bacteria was greater than that of the pH 8-bacteria in alkaline environments. Considerable difference in protein composition was observed between the membranes of the pH 8- and 10-bacteria while no difference was observed in phospholipid composition.
D000621:Aminoisobutyric Acids / Q000378:metabolism; D001407:Bacillus / Q000378:metabolism*; D001426:Bacterial Proteins / Q000096:biosynthesis; D001693:Biological Transport, Active; D002462:Cell Membrane / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D010743:Phospholipids / Q000032:analysis
D000621:Aminoisobutyric Acids; D001426:Bacterial Proteins; D010743:Phospholipids