Mediated transport and metabolism of lactate in rat aorta.
1. Under appropriate conditions L- and D-lactate enter the cells of rat aorta and are metabolized. Oxidation of lactate to CO2 occurs under aerobic conditions. 2. L- and D-lactate are taken up into the cells when oxygen, glucose, or both oxygen and glucose are present in the incubation medium. Both L- and D-lactate are excluded from the cells when neither oxygen nor glucose is present. 3. D,L-Glyceraldehyde prevents the uptake of L-lactate. The effect is apparently not due to the inhibition of glucose metabolism by L-glyceraldehyde. 4. L-lactate (20 mM) markedly inhibits the uptake of 5 mM D-lactate, but 20 mM D-lactate fails to inhibit the uptake of 5 mM L-lactate. 5. Raising the pH of the incubation medium markedly depresses the uptake of L-lactate. 6. The results provide evidence that L- and D-lactate enter the cells of rat aorta by a mediated transport system.
D000818:Animals; D001011:Aorta / Q000378:metabolism*; D002245:Carbon Dioxide / Q000378:metabolism; D004140:Dinitrophenols / Q000494:pharmacology; D005260:Female; D005947:Glucose / Q000494:pharmacology; D005985:Glyceraldehyde / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007773:Lactates / Q000378:metabolism*; D010101:Oxygen Consumption; D051381:Rats; D013237:Stereoisomerism
D004140:Dinitrophenols; D007773:Lactates; D002245:Carbon Dioxide; D005985:Glyceraldehyde; D005947:Glucose