Improved method for fractionating gamma-glutamyltransferase by electrophoresis on cellulose acetate.
I describe a method of fractionating gamma-glutamyltransferase on cellulose acetate. The tracing is obtained in parallel with that of serum protein, and the gamma-glutamyltransferase bands are characterized by correspondence with the major protein fractions. The overall pattern of the isoenzyme activity in normal sera is one of activity in the alpha1- and alpha2-globulin regions. In hepatic diseases four bands are usually present, but some more specific observations are possible, e.g., the presence of an intense beta-globulin band in occlusive icterus, intra- or extrahepatic, and a marked alpha2-globulin band in alcoholism. The potentialities of this technique as a diagnostic and prognostic aid together with its simplicity prompt me to recommend its use in the clinical laboratory.
D004588:Electrophoresis, Cellulose Acetate / Q000379:methods; D006801:Humans; D008107:Liver Diseases / Q000201:enzymology; D010182:Pancreatic Diseases / Q000201:enzymology; D005723:gamma-Glutamyltransferase / Q000097:blood / Q000302:isolation & purification*
D005723:gamma-Glutamyltransferase