Coordination chemistry of microbial iron transport compounds: rhodotorulic acid and iron uptake in Rhodotorula pilimanae.
The mechanism by which iron uptake is facilitated by the siderophore rhodotorulic acid (RA) in the yeast Rhodotorula pilimanae was investigated with radioactively labeled Fe and RA and kinetically inert, chromic-substituted RA complexes. The weight of the evidence supports a model in which RA mediates iron transport to the cell but does not actually transport iron into the cell. It is proposed that RA exchanges the ferric ion at the cell surface with a membrane-bound chelating agent that completes the active transport of iron into the cell. Uptake of 55Fe in ferric rhodotorulate was much more rapid than uptake of RA itself. Two exchange-inert chromic complexes of RA showed no uptake.
D001693:Biological Transport, Active / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D006877:Hydroxamic Acids / Q000378:metabolism; D007501:Iron / Q000378:metabolism*; D007502:Iron Chelating Agents / Q000378:metabolism; D003904:Mitosporic Fungi / Q000378:metabolism*; D008956:Models, Chemical; D010879:Piperazines / Q000378:metabolism*; D012248:Rhodotorula / Q000378:metabolism*; D013696:Temperature
D006877:Hydroxamic Acids; D007502:Iron Chelating Agents; D010879:Piperazines; C003305:rhodotorulic acid; D007501:Iron