Concentration and purification of enteroviruses by membrane chromatography.
A simple procedure for the concentration and partial purification of enteroviruses from tissue culture harvests is described. After removal of acid-precipitating components with a cationic detergent, the detergent and most membrane-coating components were removed by treatment with a cationic-exchange resin. The resin effluent was then acidified, and the virus was adsorbed to epoxy-fiberglass membranes. Virus was then eluted with pH 11.5 glycine-NaOH buffer. Since this eluate contains no orgcentrated simply by acidifying the eluate and passing it through a smaller membrane than that used for the first concentration. As high as 500-fold concentrations can be achieved, with a high efficiency of recovery.
D000327:Adsorption; D002118:Calcium / Q000494:pharmacology; D002845:Chromatography / Q000379:methods*; D046508:Culture Techniques; D004770:Enterovirus / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D007475:Ion Exchange Resins; D008274:Magnesium / Q000494:pharmacology; D008851:Micropore Filters
D007475:Ion Exchange Resins; D008274:Magnesium; D002118:Calcium