Stereospecificity of the hydrogen transfer catalyzed by human placental aldose reductase.
Placental aldose reductase (EC 1.1.1.21) was incubated with glucose in the presence of [4A-2H] NADPH prepared in the oxidation of [2-2H] isocitrate by isocitrate dehydrogenase (EC 1.1.1.42) or [4B-2H] NADPH prepared in the oxidation of [1-2H] glucose-6-phosphate dehydrogenase (EC 1.1.1.49). The sorbitol formed from [4A-2H] NADPH contained deuterium and from [4B-2H] NADPH it did not. Therefore, aldose reductase in an A-type enzyme.
D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D000449:Aldehyde Reductase / Q000378:metabolism*; D003903:Deuterium; D005260:Female; D005954:Glucosephosphate Dehydrogenase; D006801:Humans; D007521:Isocitrate Dehydrogenase; D007553:Isotope Labeling; D013058:Mass Spectrometry; D009249:NADP; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D013012:Sorbitol / Q000378:metabolism; D013329:Structure-Activity Relationship
D013012:Sorbitol; D009249:NADP; D003903:Deuterium; D000429:Alcohol Oxidoreductases; D000449:Aldehyde Reductase; D007521:Isocitrate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase