The effects of antipyretics on metabolism processes in rat liver mitochondria. Part I. The action of sodium salicylate, and pyrazolones on the reaction of respiratory chain.
The effects of sodium salicylate (SS), phenazone (Ph) and aminophenazone (APh) on mitochondrial respiration respiration and oxidative phosphorylation were studied in vitro, SS inhibited state 3 but stimulated state 4 of respiration, if alpha-ketoglutarate and succinate were used as the substrates. The inhibition of state 3 of respiration was not reversed by 2,4-dinitrophenol (DNP). Ph and APh inhibited the respiratory state 3 without affecting the state 4 in the presence of the same substrates, and the produced inhibition in state 3 was reversible by DNP. Studies on ATP synthesis have revealed that SS was the only antypyretic tested that exerted an uncoupling action. SS stimulated the mitochondrial ATPase activity but inhibited it after uncoupling of oxidative phosphorylation with DNP. The mitochondrial ATPase activity remained uninfluenced by Ph or APh. The only similar action of all the drugs investigated was the inhibition of oxidation in the respiratory state 3.
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000632:Aminopyrine / Q000494:pharmacology; D000700:Analgesics / Q000494:pharmacology*; D000818:Animals; D000983:Antipyrine / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007700:Kinetics; D008930:Mitochondria, Liver / Q000201:enzymology / Q000378:metabolism*; D010085:Oxidative Phosphorylation / Q000187:drug effects*; D010101:Oxygen Consumption / Q000187:drug effects*; D051381:Rats; D012459:Salicylates / Q000494:pharmacology
D000700:Analgesics; D012459:Salicylates; D000632:Aminopyrine; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; D000983:Antipyrine