Serum alanine assay with an enzymic micromethod (L-alanine dehydrogenase).
L-Alanine was measured by an enzymic micromethod in serum after treatment with perchloric acid, as well as after ultrafiltration through collodium membrane filters. Serum L-alanine was also determined by an automated column chromatographic technique. Using the enzymic method, spuriously increased L-alanine levels could be obtained due to the absorbent reaction between hydrazine and NAD-containing reagents. It could be shown that this absorbent reaction depends on the pH value of the test solution. In contrast to supernatant diluted by perchloric acid, ultrafiltrates gave L-alanine data equivalent to those by column chromatography, since conditions of reaction time and pH value could be kept identical for blank, standard and ultrafiltrate solutions.
D000328:Adult; D000409:Alanine / Q000097:blood*; D000594:Amino Acid Oxidoreductases; D006801:Humans; D006834:Hydrazines; D006863:Hydrogen-Ion Concentration; D008297:Male; D008722:Methods; D009243:NAD
D006834:Hydrazines; D009243:NAD; D000594:Amino Acid Oxidoreductases; D000409:Alanine