[Isolation of the islands of Langerhans from rat pancreas and determination of proteolytic activity in them].
A modified method for isolation of Langerhans islands from rat pancreas is described. The islands obtained were free from acinous tissue as shown by means of an amylase test and by microscopy. In homogenates of the islands proteolytic activities were observed (pH optimum at 5.3--5.5 and 6.9--7.2).
D000681:Amylases / Q000032:analysis; D000818:Animals; D002268:Carboxypeptidases / Q000032:analysis; D002498:Centrifugation; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D007515:Islets of Langerhans / Q000032:analysis / Q000201:enzymology*; D010447:Peptide Hydrolases / Q000032:analysis*; D051381:Rats; D014020:Tissue Extracts / Q000302:isolation & purification
D003470:Culture Media; D014020:Tissue Extracts; D000681:Amylases; D002268:Carboxypeptidases; D010447:Peptide Hydrolases
Vydelenie ostrovkov Langergansa iz podzheludochnoĭ zhelezy krys i opredelenie v nikh proteoliticheskoĭ aktivnosti