Isoelectric focusing of herpes simplex virus.
Herpes simplex virus was inserted into a preformed stable pH gradient and electrofocused for about one hour. Dextran was used as the density gradient forming agent. Virions banded at pH 4.9+/-0.1 and nucleocapsids at 4.1+/-0.05. About 10-20 per cent of infective virus was recovered.
D002460:Cell Line; D003911:Dextrans; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D018139:Simplexvirus / Q000032:analysis* / Q000472:pathogenicity; D014774:Virulence
D003911:Dextrans