PH-dependent changes of 2,3-bisphosphoglycerate in human red cells during transitional and steady states in vitro.
A systematic study of the pH-dependent changes in the range 6.6--7.4 of 2,3-bisphosphoglycerate and the adenine nucleotides was performed in the presence and absence of glucose during transitional and steady states. 1. The results indicatethat 2,3-gisphosphoglycerate phosphatase breaks down 2,3-bisphosphoglycerate nearly independent of pH at a rate of 480 mumol 2,3-bisphosphoglycerate x1 cells-1xh-1.2,3-Bisphosphoglycerate mutase is practically completely inhibited below pH value increases in long-term experiments with lower 2,3-bisphosphoglycerate levels. The formation of pyruvate corresponds to the breakdown of 2,3-bisphosphoglycerate afterconsumption of an unknown reducing substance.
D000244:Adenosine Diphosphate / Q000097:blood; D000249:Adenosine Monophosphate / Q000097:blood; D000255:Adenosine Triphosphate / Q000097:blood; D001786:Blood Glucose / Q000378:metabolism; D004163:Diphosphoglyceric Acids / Q000097:blood*; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology; D006019:Glycolysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007773:Lactates / Q000097:blood; D011773:Pyruvates / Q000097:blood
D001786:Blood Glucose; D004163:Diphosphoglyceric Acids; D007773:Lactates; D011773:Pyruvates; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D005947:Glucose