[Properties of urease immobilized by chemical binding to a derivatives of cellulose].
A comparative study of properties of soluble urease and urease immobilized by its binding to cellulose derivatives was carried out. Upon chemical binding of urease to cellulose derivatives the Mikhaelis constant and pH optimum for the enzyme action changed insignificantly. Maximum enzymic activity of soluble and immobilized urease occurred at 50 degrees C. The energy of activation of urea hydrolysis by urease during enzyme immobilization also changed insignificantly.
D002384:Catalysis; D002482:Cellulose / Q000031:analogs & derivatives; D004800:Enzymes, Immobilized; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D014510:Urease
D004800:Enzymes, Immobilized; D002482:Cellulose; D014510:Urease
Issledovanie svoĭstv ureazy, immobilizovannoĭ putem khimicheskogo prisoedineniia k proizvodnym tselliulozy.