N-Hydroxylation of phenacetin by hamster liver microsomes.
Hamster liver microsomes have been shown to catalyze the N-hydroxylation of phenacetin. The reaction, which requires oxygen and NADPH, is inhibited by a carbon monoxide/oxygen atmosphere, indicating that it is catalyzed by a cytochrome P-450-dependent mixed-function oxidase. The N-hydroxyphenacetin can be further metabolized by the microsomes, and the reaction is inhibited by phenacetin.
D000818:Animals; D003035:Cobalt / Q000494:pharmacology; D006224:Cricetinae; D006358:Hot Temperature; D006900:Hydroxylation; D066298:In Vitro Techniques; D008297:Male; D013058:Mass Spectrometry; D008647:Mesocricetus; D008748:Methylcholanthrene / Q000494:pharmacology; D008862:Microsomes, Liver / Q000378:metabolism*; D009249:NADP / Q000494:pharmacology; D010615:Phenacetin / Q000378:metabolism* / Q000494:pharmacology; D010634:Phenobarbital / Q000494:pharmacology; D010882:Piperonyl Butoxide / Q000494:pharmacology
D003035:Cobalt; D009249:NADP; D008748:Methylcholanthrene; D010615:Phenacetin; D010882:Piperonyl Butoxide; D010634:Phenobarbital