Demonstration of the phosphorylation of acetyl-coenzyme A carboxylase within intact rat epididymal fat-cells.
Intact rat epididymal fat-cells were incubated with 32Pi and the intracellular proteins separated by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. One of the phosphorylated proteins has the same RF value as [14C]biotin-labelled acetyl-CoA carboxylase purified from fat-cells and is specifically precipitated after incubation with antiserum raised against acetyl-CoA carboxylase. No significant changes in the extent of phosphorylation of acetyl-CoA carboxylase were detected after exposure of the cells to insulin.
D000103:Acetyl-CoA Carboxylase / Q000276:immunology / Q000378:metabolism*; D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D001345:Autoradiography; D004822:Epididymis / Q000378:metabolism; D066298:In Vitro Techniques; D008025:Ligases / Q000378:metabolism*; D008297:Male; D010710:Phosphates / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism; D051381:Rats
D010710:Phosphates; D011506:Proteins; D008025:Ligases; D000103:Acetyl-CoA Carboxylase