Semiautomated turbidimetric microbiological assay for cefazaflur.
A quantitative semi-automated turbidimetric bioassay for cefazaflur, using Streptococcus faecium as the indicator, is described. Assays were run at pH 6.5 approximately 7 for 3.75 hours at 37 degrees C using 2 approximately 12 microgram cefazaflur per ml assay broth for standards. The dose response line was plotted point to point using the natural log of the absorbance vs natural log of the concentration. This assay is both accurate and precise and is more rapid than traditional plate assays for antibiotics.
D001681:Biological Assay / Q000379:methods; D002511:Cephalosporins / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D009391:Nephelometry and Turbidimetry; D013997:Time Factors
D002511:Cephalosporins