[Applications of the extraction and of the radioimmunoassay of LH-RH in human urine].
The existence of endogenous LH RH like immunoreactivity is shown in human urines after appropriate extraction, bu the radioimmunoassay of LH RH. In normaly cycling and menopausal women the quantities of endogenous hormone found in urines are greater after acid extraction than those found after extraction at pH 7. Furthermore, the increase observed by extraction in acidified methanol is directly correlated and proportional to the quantity of hormone assayable by extraction at pH 7. The hypothesis of urinary excretion of LH RH as a polymer of immunoreactive units is suggested by this study.
D000368:Aged; D000818:Animals; D004971:Estrus; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000302:isolation & purification / Q000652:urine*; D006728:Hormones / Q000652:urine; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008593:Menopause; D000432:Methanol; D008875:Middle Aged; D011108:Polymers; D011247:Pregnancy; D011863:Radioimmunoassay
D006728:Hormones; D011108:Polymers; D007987:Gonadotropin-Releasing Hormone; D000432:Methanol
Applications de l'extraction et du dosage radioimmunologique du lh-rh dans les urines humaines