Beta-glucanases in the yeast Cryptococcus albidus var. aerius. Production and separation of beta-glucanases in asynchronous cultures.
beta-Glucanases were detected in cell-free extracts of the yeast Cryptococcus albidus var. aerius when grown on glucose as the sole carbon source. The production of beta-glucanases was followed in log-phase cells and stationary-phase cells; the maximal production of beta-(1 leads to 3) and beta-(1 leads to 6) glucanases takes place respectively in log-phase and stationary-phase cells. The results show that there are marked differences in the elution profiles on Sephadex G-50 of fractions containing beta-glucanase from cells grown for 12, 24, 48, 72, and 96 h. The possibility either of replacement changes in fractions containing beta-glucanase activity or of a different synthesis of each beta-glucanase during the growth of the yeast is discussed. The results suggest that all fractions containing beta-glucanases hydrolyze both beta-(1 leads to 3) and beta-(1 leads to 6) linkages. Evidence in support of the conclusion that a low molecular form of beta-glucanase has a molecular weight of 2100 +/- 100 is also shown.
D002474:Cell-Free System; D002850:Chromatography, Gel; D003454:Cryptococcus / Q000201:enzymology* / Q000254:growth & development; D004492:Edetic Acid / Q000494:pharmacology; D004693:Glucan Endo-1,3-beta-D-Glucosidase / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D005947:Glucose / Q000096:biosynthesis; D006026:Glycoside Hydrolases / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D011092:Polyethylene Glycols / Q000494:pharmacology; D011134:Polysaccharides / Q000378:metabolism
D011134:Polysaccharides; D011092:Polyethylene Glycols; D004492:Edetic Acid; D006026:Glycoside Hydrolases; D004693:Glucan Endo-1,3-beta-D-Glucosidase; D005947:Glucose