Studies on the cell cycle of Myxobacter AL-1. II. Activities of seven enzymes during the cell cycle.
The properties of seven enzymes were studied in extracts from Myxobacter AL-1. The enzymes were isocitrate dehydrogenase (E.C.1.1.1.42), succinate dehydrogenase (E.C.1.3.99.1), alkaline phosphatase (E.C.3.1.3.1), alpha-glucosidase (E.C.3.2.1.20), beta-glucosidase (E.C.3.2.1.21), beta-galactosidase (E.C.3.2.1.23), and N-acetyl-glucosaminidase (E.C. 3.2.1.30). Four of these enzymes: isocitrate dehydrogenase, alpha-glucosidase, beta-glucosidase, and beta-galactosidase are cytosolic enzymes. Succinate dehydrogenase was found to be located on the cytoplasmic membrane system, whereas alkaline phosphatase and N-acetylglucosaminidase were considered as enzymes which bind the outer membranes resp. the cell wall. During the cell cycle, all enzymes have a pattern of discontinuous activity increase. Succinate dehydrogenase and isocitrate dehydrogenase exhibit a stepwise increase of activity, whereas the other enzymes follow the pattern of a peak enzyme.
D000118:Acetylglucosaminidase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D002453:Cell Cycle; D002462:Cell Membrane / Q000201:enzymology; D002473:Cell Wall / Q000201:enzymology; D003600:Cytosol / Q000201:enzymology; D006863:Hydrogen-Ion Concentration; D006867:Hydrolases / Q000378:metabolism*; D007521:Isocitrate Dehydrogenase / Q000378:metabolism; D009231:Myxococcales / Q000166:cytology / Q000201:enzymology*; D010088:Oxidoreductases / Q000378:metabolism*; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013696:Temperature; D000520:alpha-Glucosidases / Q000378:metabolism; D001617:beta-Glucosidase / Q000378:metabolism
D010088:Oxidoreductases; D007521:Isocitrate Dehydrogenase; D013385:Succinate Dehydrogenase; D006867:Hydrolases; D000469:Alkaline Phosphatase; D000520:alpha-Glucosidases; D001617:beta-Glucosidase; D000118:Acetylglucosaminidase