A method for the measurement of L-phenylalanine mustard in the mouse and dog by high-pressure liquid chromatography.
The distribution of L-phenylalanine mustard (L-PAM) was studied in dogs and mice by high-pressure liquid chromatography. Separation of L-PAM from its products of hydrolysis was accomplished with a mu-Bondapak C18 column, a solvent system composed of 2-methoxyethanol/0.1% acetic acid, and solvent programming with a step gradient. Complete separation was effected in less than 15 min. The half-life for disappearance of L-PAM from mouse blood was 41 min, whereas that from dog blood was 29 min. The monohydroxy derivative of L-PAM, L-MOH, disappeared from dog serum with a half-life of 32 min. L-MOH was not detectable in mouse tissue other than blood at times greater than 15 min after injection. In the dog at 4 hr after injection, the tissue/serum concentration ratios were greater than 1 for liver, spleen, intestine, skeletal muscle, urinary bladder and gallbladder. The concentration of L-PAM in the bile was approximately 500 times higher than that in serum.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D006207:Half-Life; D006868:Hydrolysis; D008297:Male; D008558:Melphalan / Q000032:analysis* / Q000378:metabolism; D008722:Methods; D051379:Mice; D008815:Mice, Inbred Strains; D013997:Time Factors
D008558:Melphalan