Optimal conditions for elution of hepatitis B antigen after absorption onto colloidal silica.
Hepatitis B surface antigen (HBSAg) adsorbed from sera onto colloidal silica could be completely eluted through the use of 0.25% sodium deoxycholate in 0.01 M borax, pH 9.3, at 56 degrees C. The HBSAg recovered in the eluate represented 100% of that present in the original serum, and it was contaminated by only trace amounts of serum proteins (in decreasing amounts: beta-lipoprotein, immunoglobulin G, albumin). This preliminary step greatly facilitates purification of large amounts of HBSAg and provides small volumes of highly concentrated material for subsequent purification by density gradient centrifugation.
D000042:Absorption; D001769:Blood / Q000382:microbiology; D003102:Colloids; D003840:Deoxycholic Acid; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008722:Methods; D012822:Silicon Dioxide; D013696:Temperature; D014765:Viral Vaccines
D003102:Colloids; D014765:Viral Vaccines; D003840:Deoxycholic Acid; D012822:Silicon Dioxide