Natural pH gradients in buffer mixtures: formation in the absence of strongly acidic and basic anolyte and catholyte, gradient steepening by sucrose, and stabilization by high buffer concentrations in the electrolyte chambers.
D000613:Aminobutyrates; D002021:Buffers; D006863:Hydrogen-Ion Concentration; D006878:Hydroxides; D007525:Isoelectric Focusing; D013395:Sucrose; D013451:Sulfonic Acids; D013464:Sulfuric Acids
D000613:Aminobutyrates; D002021:Buffers; D006878:Hydroxides; D013451:Sulfonic Acids; D013464:Sulfuric Acids; D013395:Sucrose