On the separation of fibrinogen degradation products D and E.
Separation of fibrinogen degradation products D and E by means of gel chromatography cannot be achieved at neutral pH even in the presence of high ionic strength of the elution buffer. It is assumed that fragments D and E are linked together in a complex preventing the separation despite different molecular weights of both components. By means of addition of chaotropic substances like 1 M Kl to the elution buffer clear separation of degradation products D and E on Sephadex G-200 columns can be achieved.
D001665:Binding Sites; D002850:Chromatography, Gel; D004589:Electrophoresis, Disc; D005338:Fibrin Fibrinogen Degradation Products; D006863:Hydrogen-Ion Concentration; D007122:Immunoelectrophoresis; D008970:Molecular Weight; D010446:Peptide Fragments / Q000302:isolation & purification; D011485:Protein Binding
D005338:Fibrin Fibrinogen Degradation Products; D010446:Peptide Fragments