Partial characterization of prostaglandin synthetase in the reproductive tract of the male house cricket, Acheta domesticus.
An active prostaglandin (PG) synthetase was found in the 12100 g pellet of reproductive tract homogenates of the male house cricket, Acheta domesticus. Comparatively, the 12100 g supernatant and the microsomal fractions were inactive. The PG synthetase in the pellet fraction was characterized in terms of cofactor, temperature, pH, and incubation time requirements. Indomethacin, a known inhibitor of mammalian PG synthetase, was not inhibitory to the cricket synthetase. The procedure and findings are relevant to PG synthetase studies of any organism or tissue.
D000818:Animals; D005837:Genitalia, Male / Q000201:enzymology*; D005978:Glutathione / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007213:Indomethacin / Q000494:pharmacology; D007313:Insecta / Q000201:enzymology*; D008297:Male; D008861:Microsomes / Q000201:enzymology; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D011451:Prostaglandin-Endoperoxide Synthases / Q000032:analysis / Q000378:metabolism*; D011458:Prostaglandins E / Q000096:biosynthesis; D011460:Prostaglandins F / Q000096:biosynthesis; D013696:Temperature; D013997:Time Factors
D011458:Prostaglandins E; D011460:Prostaglandins F; D006899:Mixed Function Oxygenases; D011451:Prostaglandin-Endoperoxide Synthases; D005978:Glutathione; D007213:Indomethacin