Effect of concanavalin A on black widow spider venom activity at the neuromuscular junction: implications for mechanisms of venom action.
Concanavalin A (Con A) inhibits black widow spider venom-induced transmitter release at both tissue-cultured and adult neuromuscular junctions and also inhibits the venom-induced destruction of cultured neurites. This inhibitory action is partially or completely prevented by prior treatment with colchicine. Neither colchicine nor Con A interacts significantly with depolarization-induced transmitter release. These results are analogous to those obtained from experiments on lymphocyte surface receptor capping. They suggest that redistribution of neuronal membrane components may be a crucial step in spider venom action. This membrane redistribution appears to be modulated in neurons, as in other cell types, by microtubule-microfilament array. How such a redistribution causes increased transmitter release cannot as yet be specified. Changes in the ionic permeability of sodium and potassium were examined as likely mechanisms. Increased sodium influx (and seondary release of calcium from intracellular stores) cannot be the basis for spider venom action. Increased potassium efflux remains a possibility, but is not consistent with all of the data. Other possible mechanisms are also suggested.
D000818:Animals; D001001:Anura; D001180:Arthropod Venoms / Q000037:antagonists & inhibitors*; D001740:Black Widow Spider; D002462:Cell Membrane / Q000187:drug effects; D002463:Cell Membrane Permeability / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D003078:Colchicine / Q000494:pharmacology; D003208:Concanavalin A / Q000494:pharmacology*; D008766:Methylmannosides / Q000494:pharmacology; D009469:Neuromuscular Junction / Q000187:drug effects*; D009474:Neurons / Q000187:drug effects; D018377:Neurotransmitter Agents / Q000378:metabolism; D011188:Potassium / Q000494:pharmacology; D012964:Sodium / Q000494:pharmacology; D013111:Spider Venoms / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D013112:Spiders; D013570:Synaptic Membranes / Q000187:drug effects
D001180:Arthropod Venoms; D008766:Methylmannosides; D018377:Neurotransmitter Agents; D013111:Spider Venoms; D003208:Concanavalin A; D012964:Sodium; D011188:Potassium; D003078:Colchicine