Inside-out membrane vesicles isolated from spinach thylakoids.
Inside-out thylakoid vesicles have been separated from right-side-out material after press disruption of chloroplast lamellae. The sepration was obtained by partitionin an aqueous dextran-polyethylene glycol two-phase system, a method which utilizes differences in surface properties for separation of membrane particles. The isolated thylakoid vesicles showed the following inside-out properties: (1) light-induced reversible proton extrusion into the surrounding medium when supplied with the Photosystem II electron acceptor phenyl-p-benzoquinone; (2) a pH rise in the internal phase accompanying the external proton release, (3) sensitivity to trypsin treatment different from that of thylakoid membranes of normal orientation; (4) concave EF and convex PF freeze-fracture faces.
D002736:Chloroplasts / Q000187:drug effects / Q000378:metabolism*; D004237:Diuron / Q000494:pharmacology; D005614:Freeze Fracturing; D006863:Hydrogen-Ion Concentration; D007425:Intracellular Membranes / Q000378:metabolism / Q000648:ultrastructure; D007700:Kinetics; D010100:Oxygen / Q000378:metabolism; D010788:Photosynthesis / Q000187:drug effects; D010944:Plants / Q000378:metabolism*
D004237:Diuron; D010100:Oxygen