The effect of an NADPH-regenerating system on biphenyl metabolism in isolated rat hepatocytes.
Biphenyl 4-hydroxylation was studied in isolated rat hepatocytes. It was found that there was in inter-relationship between 4-hydroxylase activity and glucuronidase activity, removal of 4-hydroxybiphenyl by conjugation being necessary to stimulate a second phase of hydroxylation. Addition of an NADPH-regenerating system resulted in an initial depression of both processes, but later their activities were enhanced. This action could not be explained by the presence of non-viable cells.
D000818:Animals; D001713:Biphenyl Compounds / Q000378:metabolism*; D002470:Cell Survival; D005965:Glucuronates / Q000378:metabolism; D005966:Glucuronidase / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008297:Male; D006899:Mixed Function Oxygenases / Q000378:metabolism; D009249:NADP / Q000494:pharmacology*; D051381:Rats
D001713:Biphenyl Compounds; D005965:Glucuronates; D009249:NADP; D006899:Mixed Function Oxygenases; D005966:Glucuronidase