A new factor from enteric bacteria of rats amplifying induction of liver enzyme by glucocorticoid. 1. Purification, properties and biological action.
1. A factor, which amplifies the inductions of several liver enzymes by glucocorticoid, was partially purified from Proteus mirabilis from rat intestine. The factor (amplifier) was completely inactivated by alpha-glucosidase, but not by other glycoside hydrolases, proteases, nucleases or phosphatases tested; it was also hydrolysed by HCl with liberation of reducing sugars. Thus the oligosaccharide in this factor seems to be essential for the amplification. 2. In adrenalectomized rats the amplifier increased the inductions of several liver enzymes, such as tyrosine aminotransferase and leucine aminotransferase, by glucocorticoid. But it did not amplify the induction of tyrosine aminotransferase by glucagon or insulin or the activities of enzymes that are not induced by glucocorticoid. The amplifier by itself did not have any glucocorticoid-like action in adrenalectomized rat. These results show that the amplifier specifically increases the inductions of liver enzymes by glucocorticoid. 3. Since similar amplification was also observed in isolated perfused liver and cultured hepatoma cells in vitro, the amplifier seems to act directly on the target organ or cells.
D000818:Animals; D001426:Bacterial Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D002460:Cell Line; D003907:Dexamethasone / Q000494:pharmacology*; D004355:Drug Stability; D004357:Drug Synergism; D004790:Enzyme Induction / Q000187:drug effects; D005934:Glucagon / Q000494:pharmacology; D006023:Glycoproteins / Q000302:isolation & purification / Q000494:pharmacology*; D007328:Insulin / Q000494:pharmacology; D007422:Intestines / Q000382:microbiology*; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008297:Male; D010477:Perfusion; D011513:Proteus mirabilis / Q000032:analysis*; D051381:Rats; D014444:Tyrosine Transaminase / Q000096:biosynthesis*
D001426:Bacterial Proteins; D006023:Glycoproteins; D007328:Insulin; D003907:Dexamethasone; D005934:Glucagon; D014444:Tyrosine Transaminase