Endo-arabinanase from Bacillus subtilis F-11.
An arabinanase was purified from the culture fluid of Bacillus subtilis F-11. The process was as follows: salting out by (NH4)2SO4, repeated chromatography on hydroxy apatite and gel filtration on Sepharose-6B. The purified enzyme was demonstrated to be homogeneous by disc electrophoresis. The enzyme was found to be active on arabinan and 1,5-arabinan, but inactive on phenyl alpha-L-arabinofuranoside, p-nitrophenyl beta-D-galactopyranoside, arabinoxylan, gum arabic. The enzyme released arabinose, arabinobiose, arabinotriose and higher oligosaccharides during the course of hydrolysis of 1,5-arabinan. The end products were found to be arabinose and arabinobiose after 144 h of hydrolysis.
D001089:Arabinose; D001412:Bacillus subtilis / Q000201:enzymology*; D002850:Chromatography, Gel; D005960:Glucosides / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008628:Mercury / Q000494:pharmacology; D009598:Nitrophenylgalactosides / Q000378:metabolism; D009844:Oligosaccharides / Q000378:metabolism; D013329:Structure-Activity Relationship
D005960:Glucosides; D009844:Oligosaccharides; D009598:Nitrophenylgalactosides; D001089:Arabinose; D006026:Glycoside Hydrolases; D008628:Mercury