High-voltage paper electrophoretic assay for guanyl cyclase.
A simple, sensitive and rapid technique is described, permitting separation of cGMP from GMP, GDP and GTP by the use of unidirectional high-voltage paper electrophoresis. The recovery of labeled cGMP in the assay of guanyl cyclase, by this procedure is 85-90%; the blank values (no enzyme) are negligible.
D000818:Animals; D006152:Cyclic GMP / Q000032:analysis; D004590:Electrophoresis, Paper / Q000379:methods; D006150:Guanine Nucleotides / Q000032:analysis; D006162:Guanylate Cyclase / Q000032:analysis*; D051381:Rats
D006150:Guanine Nucleotides; D006162:Guanylate Cyclase; D006152:Cyclic GMP