Latency of microsomal hexose-6-phosphate dehydrogenase activity.
Intact microsomes isolated from rat liver showed no hexose-6-phosphate dehydrogenase activity, but the enzyme was activated by Triton X-100, deoxycholate, NH4OH, glycine/NaOH, lysophosphatidylcholine, phospholipases A and C, pancreatic lipase and cholesterol esterase, and also by sonic treatment. The enzyme activation by deoxycholate, NH4OH and sonic treatments was solely due to solubilization, while that by phospholipase A appeared to be due to the detergent action of the hydrolysis products. On the other hand, the primary effects of phospholipase C, cholesterol esterase and pancreatic lipase might be accounted for by the partial removal of membrane lipids. The results of washing and trypsin digestion experiments suggested that hexose-6-phosphate dehydrogenase is one of the most firmly bound enzymes among the microsomal proteins. The catalytic properties were the same in the solubilized and the membrane-bound, activated enzymes. Feeding the rats on a high carbohydrate diet altered the extent of enzyme activation by sonication and phospholipase C treatment, suggesting that the microsomal membrane would actually undergo changes in the conformation and/or chemical composition under certain circumstances.
D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D000468:Alkalies / Q000494:pharmacology; D000818:Animals; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism; D002474:Cell-Free System; D003902:Detergents / Q000494:pharmacology; D004040:Dietary Carbohydrates / Q000378:metabolism; D004789:Enzyme Activation; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D006600:Hexosephosphates; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008055:Lipids; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D009249:NADP / Q000378:metabolism; D010634:Phenobarbital / Q000494:pharmacology; D051381:Rats; D012995:Solubility; D013010:Sonication
D000468:Alkalies; D003902:Detergents; D004040:Dietary Carbohydrates; D006600:Hexosephosphates; D008055:Lipids; D009249:NADP; D000429:Alcohol Oxidoreductases; D005954:Glucosephosphate Dehydrogenase; D002265:Carboxylic Ester Hydrolases; D010634:Phenobarbital