Albumin/calcium association at different pH, as determined by potentiometry.
Calcium binding by albumin was determined potentiometrically at physiological ionic strength and temperature as a function of pH. The binding data indicate at least 30 different binding sites with different association constants and different H+ interaction. One site appears to be responsible for the major binding at physiological pH and substance concentration of free calcium, together with three other sites that bind with less affinity.
D002118:Calcium; D006801:Humans; D006863:Hydrogen-Ion Concentration; D011199:Potentiometry / Q000379:methods; D011485:Protein Binding; D012709:Serum Albumin; D013816:Thermodynamics
D012709:Serum Albumin; D002118:Calcium