Surface-charge characteristics of smooth and rough Salmonella typhimurium bacteria determined by aqueous two-phase partitioning and free zones electrophoresis.
Aqueous biphasic partitioning of Salmonella typhimurium S and R bacteria in a system containing 6.2 per cent (w/w) dextran 500 and 4.4 per cent (w/w) poly(ethyleneglycol) 6000 (PEG) was similar to the partition of the corresponding surface lipopolysaccharide (LPS). Further partition analysis with charged PEG showed that S bacteria and their LPS exposed very little charge, whereas R bacteria and their LPS showed a conspicuous negative charge at neutral pH. Free zone electrophoresis also indicated that the S bacteria have a much lower surface charge density than the R bacteria and accordingly a different surface structure. Thus, the physico-chemical properties of the bacterial surface seem to be determined to a great extent by the characteristics of the cell surface LPS.
D002473:Cell Wall / Q000032:analysis; D003378:Counterimmunoelectrophoresis; D003911:Dextrans; D006863:Hydrogen-Ion Concentration; D007123:Immunoelectrophoresis, Two-Dimensional; D008070:Lipopolysaccharides / Q000032:analysis; D011092:Polyethylene Glycols; D011135:Polysaccharides, Bacterial / Q000032:analysis; D012486:Salmonella typhimurium / Q000032:analysis
D003911:Dextrans; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D011092:Polyethylene Glycols