[Tissue specific inhibition of lymphocyte proliferation by spleen extract (lymphocyte chalone) (author's transl)].
Aqueous spleen extracts were purified using acetone precipitation, membrane filtration, affinity chromatography, and dialysis. These extracts were able to inhibit thymidine incorporation into lymphoid cells (MKT-CH and PHA-stimulated lymphocyte cultures). They did not influence non lymphoid tissue (melanoma cells Mel Ei 78 and Ehrlich ascites cells). The inhibition was reversible and the purified extracts were not cytotoxic. The extracts correspond to a chalone. Their importance for prevention of graft versus host reaction and for treatment of lymphoproliferative diseases is discussed.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor; D002417:Cattle; D003601:Cytotoxicity Tests, Immunologic; D004273:DNA, Neoplasm / Q000096:biosynthesis; D006087:Graft vs Host Reaction / Q000187:drug effects; D006131:Growth Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects*; D008545:Melanoma; D008938:Mitosis / Q000187:drug effects*; D013154:Spleen; D014020:Tissue Extracts / Q000494:pharmacology
D004273:DNA, Neoplasm; D006131:Growth Inhibitors; D014020:Tissue Extracts
Gewebsspezifische Hemmung der Lymphocytenproliferation durch Milzextrakt (Lymphocytenchalon)