[Soluble high molecular weight derivatives of trypsin pancreatic inhibitor. Isolation and properties of dextran-bound pancreatic inhibitor].
A method of isolating preparations of pancreatic inhibitor of trypsin, bound with soluble polysaccharide carriers, is worked out. It is demonstrated that the reaction of a pancreatic inhibitor and cyanuric chloride-activated dextran proceeds for OH groups of tyrosine residues and for-epsilon-NH2 groups of lysine residues. A method is offered of the protection of amino groups with citraconic anhydride for the complete retaining of the inhibitory activity during attachment to dextran. Thermic denaturation of pancreatic inhibitor preparations at pH 4.7 and 97 degrees C is studied. It is found that the modification by 2-amino-4.6-dichloro-s-triazine stabilizes the protein molecule, while the interaction with the matrix of soluble dextran does not carry any contribution to thermostability of the pancreatic inhibitor.
D002949:Citraconic Anhydrides; D003911:Dextrans; D004355:Drug Stability; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D010179:Pancreas / Q000032:analysis*; D011485:Protein Binding; D011489:Protein Denaturation; D012995:Solubility; D014227:Triazines; D014361:Trypsin Inhibitors / Q000302:isolation & purification*
D002949:Citraconic Anhydrides; D003911:Dextrans; D014227:Triazines; D014361:Trypsin Inhibitors
Rastvorimye Vysokomolekuliarnye proisvodnye pankreaticheskogo ingibitora tripsina Poluchenie i svoĭstva pankreaticheskogo ingibitora, sviazannogo s dekstranom.