Dietary and hormonal regulation of the content of acetyl coenzyme A carboxylase-synthesizing polysomes in rat liver.
Specific polysomes involved the the synthesis of acetyl-CoA carboxylase [acetyl-CoA: carbon-dioxide ligase (ADP-forming), EC 6.4.1.2] have been identified by the binding of 125I-labeled antiacetyl-CoA carboxylase to rat liver polysomes. The binding is highly specific and occurs through the recognition of the nascent peptide chains on polysomes. With the use of the 125I-labeled antibody binding technique, it has been demonstrated that the relative content of acetyl-CoA carboxylase-synthesizing polysomes in the liver correlates well with the rate of hepatic synthesis of the enzyme in rats subjected to different dietary conditions as well as in alloxan-diabetic rats with or without insulin treatment.
D000103:Acetyl-CoA Carboxylase / Q000096:biosynthesis* / Q000276:immunology; D000818:Animals; D000937:Antigen-Antibody Reactions; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D004032:Diet; D005215:Fasting; D007328:Insulin / Q000494:pharmacology*; D008025:Ligases / Q000096:biosynthesis*; D008099:Liver / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D011132:Polyribosomes / Q000378:metabolism*; D051381:Rats
D007328:Insulin; D008025:Ligases; D000103:Acetyl-CoA Carboxylase