Cornea preparation for in vitro bio-pharmaceutical evaluation of ophthalmic dosage forms.
A system has been developed for a specific biopharmaceutical purpose: testing for ophthalmic preparations, the influence of formulation on drug transport through the cornea. The apparatus is a lucite cell, divided in two compartments by a clamped rabbit cornea. Physiological conditions are ensured by a supply of oxygenated perfusion medium. They are monitored by electrical conductivity and corneal thickness measurements. Reliability of the system was tested in a set of experiments.
D000818:Animals; D001702:Biopharmaceutics; D003315:Cornea / Q000378:metabolism*; D004341:Drug Evaluation; D004553:Electric Conductivity; D066298:In Vitro Techniques; D009883:Ophthalmic Solutions / Q000378:metabolism*; D010477:Perfusion / Q000295:instrumentation; D011343:Procaine / Q000378:metabolism; D011817:Rabbits; D013678:Technology, Pharmaceutical / Q000295:instrumentation*; D013997:Time Factors
D009883:Ophthalmic Solutions; D011343:Procaine