Bacterial 2,3-butanediol dehydrogenases.
Enterobacter aerogenes, Aeromonas hydrophila, Serratia marcescens and Staphylococcus aureus possessing L(+)-butanediol dehydrogenase produced mainly meso-butanediol and small amounts of optically active butanediol; Acetobacter suboxydans, Bacillus polymyxa and Erwinia carotovora containing D(-)-butanediol dehydrogenase produced more optically active butanediol than meso-butanediol. Resting and growing cells of these organisms oxidezed only one enantiomer of racemic butanediol. The D(-)-butanediol dehydrogenase from Bacillus polymyxa was partially purified (30-fold) with a specific activity of 24.5. Except NAD and NADH no other cofactors were required. Optimum pH-values for oxidation and reduction were pH 9 and pH 7, respectively. The optimum temperature was about 60 degrees C. The molecular weight was 100000 to 107000. The Km-values were 3.3 mM for D(-)butanediol, 6.25 mM for meso-butanediol, 0.53 mM for acetoin, 0.2 mM for NAD, 0.1 mM for NADH, 87 mM for diacetyl, 38 mM for 1,2-propanediol; 2,3-pentanedion was not a substrate for this enzyme. The L(+)butanediol dehydrogenase from Serratia marcescens was purified 57-fold (specific activity 22.3). Besides NAD or NADH no cofactors were required. The optimum value for oxidation was about pH9 and for reduction pH 4.5. The optimum temperature was 32-36 degrees C. The molecular weight was 100000 to 107000. The Km-values were 5 mM for meso-butanediol, 10 mM for racemic butanediol, 6.45 for acetoin, 1 mM for NAD, 0.25 mM for NADH, 2.08 mM for diacetyl, 16.7 mM for 2,3-pentanedion and 11.8 mM for 1,2-propanediol.
D000091:Acetobacter / Q000201:enzymology; D000093:Acetoin / Q000378:metabolism; D000333:Aeromonas / Q000201:enzymology; D000429:Alcohol Oxidoreductases / Q000302:isolation & purification / Q000378:metabolism*; D001407:Bacillus / Q000032:analysis / Q000201:enzymology; D001419:Bacteria / Q000201:enzymology*; D002072:Butylene Glycols / Q000378:metabolism; D002474:Cell-Free System; D004754:Enterobacter / Q000201:enzymology; D004885:Erwinia / Q000201:enzymology; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction; D012706:Serratia marcescens / Q000032:analysis / Q000201:enzymology; D013211:Staphylococcus aureus / Q000201:enzymology; D013696:Temperature
D002072:Butylene Glycols; D000093:Acetoin; D000429:Alcohol Oxidoreductases