Pyrimidine nucleotide synthesis in rat embryo cells infected with X14 or H-1 parvovirus.
The activity of some enzymes involved in pyrimidine nucleotide synthesis was studied in rat embryo cell (REC) cultures infected with X14 or H-1 parvovirus. dCMP aminohydrolase activity of the infected cells was 64--121% greater than that of the mock-infected cells. dTMP synthetase activity was 18% greater in X14 virus-infected cells and 34% lower in H-1 virus-infected cells. These results suggest some differences in the infected cells, as regards the biosynthesis of dTMP. Orotate phosphoribosyltransferase and orotidylate decarboxylase activities appeared nearly unmodified compared to the mock-infected cells. The addition of phosphoribosylpyrophosphate (PRPP) to the cell suspension incubated with [6-14C] orotate increased the specific radioactivity of acid-soluble uracil, 5-fold in the mock-infected cells and 15- --24-fold in the X14 or H-1 virus-infected cells (72 hr p.i.). This result suggests that the lowered pyrimidine nucleotide synthesis in infected cells depends to a large extent on the diminished PRPP pool.
D002262:Carboxy-Lyases / Q000378:metabolism*; D002460:Cell Line; D003596:Cytosine / Q000096:biosynthesis; D003844:DCMP Deaminase / Q000378:metabolism*; D008780:Methyltransferases / Q000378:metabolism*; D009709:Nucleotide Deaminases / Q000378:metabolism*; D009962:Orotate Phosphoribosyltransferase / Q000378:metabolism*; D009964:Orotidine-5'-Phosphate Decarboxylase / Q000378:metabolism*; D010321:Parvoviridae / Q000254:growth & development*; D010430:Pentosyltransferases / Q000378:metabolism*; D010754:Phosphoribosyl Pyrophosphate / Q000494:pharmacology; D011743:Pyrimidines / Q000096:biosynthesis*; D013940:Thymidylate Synthase / Q000378:metabolism*; D014498:Uracil / Q000096:biosynthesis
D011743:Pyrimidines; D014498:Uracil; D010754:Phosphoribosyl Pyrophosphate; D003596:Cytosine; D008780:Methyltransferases; D013940:Thymidylate Synthase; D010430:Pentosyltransferases; D009962:Orotate Phosphoribosyltransferase; D009709:Nucleotide Deaminases; D003844:DCMP Deaminase; D002262:Carboxy-Lyases; D009964:Orotidine-5'-Phosphate Decarboxylase