Purification of methylmalonyl-CoA mutase from Propionibacterium shermanii using affinity chromatography.
A novel procedure for the purification of methylmalonyl-CA mutase from Propionibacterium shermanii has been described which employs affinity chromatography on a column of immobilized vitamin B-12 linked covalently to Sepharose. The method has the advantage of being simple and rapid, thus enabling the purification of the enzyme to near homogeneity with good yields.
D002846:Chromatography, Affinity; D007535:Isomerases / Q000302:isolation & purification*; D008765:Methylmalonyl-CoA Mutase / Q000302:isolation & purification*; D011424:Propionibacterium / Q000201:enzymology*; D012685:Sepharose; D014805:Vitamin B 12
D012685:Sepharose; D007535:Isomerases; D008765:Methylmalonyl-CoA Mutase; D014805:Vitamin B 12