Optimum pH and ionic strength for the assay of cytochrome c oxidase from pea cotyledon mitochondria.
Cytochrome c oxidase (EC 1.9.3.1) has been solubilized by use of the nonionic detergents were determined for the oxidation of ferrocytochrome c in air. The results indicate that the plant cytochrome c oxidase resembles mammalian preparations in its sensitivity towards ionic strength and pH of the assay buffer.
D003574:Cytochrome c Group; D003576:Electron Transport Complex IV / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008928:Mitochondria / Q000201:enzymology; D009994:Osmolar Concentration; D010944:Plants / Q000201:enzymology*
D003574:Cytochrome c Group; D003576:Electron Transport Complex IV