Alterations of prostaglandin E2-9-ketoreductase activity in proliferating skin.
The activity of an NADPH-dependent PGE2-9-ketoreductase has been demonstrated in rat and human skin. This activity is localized in the high speed supernatant fraction, indicating the presence of an active PGE2-9-ketoreductase associated with the cytoplasmic fraction of the skin. Transformation of PGE2 into PGF2alpha is enhanced by skin specimens from psoriatic plaques and EFA-deficient rats, both characterized by excessive cellular proliferation and increased NADPH production. Incubations of the 105,000 g supernatant fractions from normal and EFA-deficient rats demonstrated that the activity of the PGE2-9-ketoreductase was elevated in high speed preparations from EFA-deficient rats. Results from these studies suggest that the increased activity of PGE2-9-ketoreductase observed in skin from human psoriatic plaques and EFA-deficient rats may be due in part to the increased generation of NADPH by these tissues and in part to alteration of the PGE2-9-ketoreductase by the excessive proliferation of the tissues.
D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D000818:Animals; D002455:Cell Division; D005228:Fatty Acids, Essential / Q000172:deficiency*; D006019:Glycolysis; D006801:Humans; D006911:Hydroxyprostaglandin Dehydrogenases / Q000378:metabolism*; D007700:Kinetics; D009243:NAD; D009249:NADP; D011458:Prostaglandins E / Q000378:metabolism; D011460:Prostaglandins F / Q000096:biosynthesis; D011565:Psoriasis / Q000201:enzymology; D051381:Rats; D012867:Skin / Q000201:enzymology* / Q000378:metabolism; D013045:Species Specificity
D005228:Fatty Acids, Essential; D011458:Prostaglandins E; D011460:Prostaglandins F; D009243:NAD; D009249:NADP; D000429:Alcohol Oxidoreductases; D006911:Hydroxyprostaglandin Dehydrogenases