Synthesis and characterization of glucuronides of 5'-hydroxy-delta9-tetrahydrocannabinol and 11-hydroxy-delta9-tetrahydrocannabinol.
Glucuronides of two metabolites of delta9-tetrahydrocannabinol, 11-hydroxy-delta9-THC and 5'-hydroxy-delta9-THC, have been synthesized and characterized. Two isomeric monoglucuronides were isolated from the incubation of each metabolite with UDP-glucuronyltransferase immobilized on beaded Sepharose. The structures of these conjugates were assigned primarily on the basis of combined gas chromatography-mass spectrometry.
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D002855:Chromatography, Thin Layer; D013759:Dronabinol / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D005965:Glucuronates / Q000138:chemical synthesis; D006900:Hydroxylation; D013058:Mass Spectrometry; D011817:Rabbits
D005965:Glucuronates; D013759:Dronabinol