[Alternativity of methane assimilation pathways in obligate methylotrophs].
The activity of key enzymes involved in the primary pathways of methane assimilation and enzymes of the citrate cycle was determined in various obligate methylotrophs: mesophilic, thermotolerant, and thermophilic. The bacteria are characterized by the membrane ultrastructure of the I type, high activity of hexosephosphate synthase, NAD- and NADP-specific isocitrate dehydrogenase, and the absence of alpha-ketoglutarate dehydrogenase. The bacteria also displayed the activity of key enzymes of the serine cycle, hydroxypyruvate reductase, and serineglyoxylate aminotransferase. Therefore, both the ribulose monophosphate and serine pathways are involved in methane assimilation, and the division of methanotrophs into two groups, according to their metabolism, is tentative.
D000446:Aldehyde-Lyases / Q000378:metabolism; D006600:Hexosephosphates / Q000096:biosynthesis; D007521:Isocitrate Dehydrogenase / Q000378:metabolism; D007655:Ketoglutarate Dehydrogenase Complex / Q000378:metabolism; D008291:Malate Dehydrogenase / Q000378:metabolism; D008697:Methane / Q000378:metabolism*; D008772:Methylococcaceae / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D011768:Pyruvate Dehydrogenase Complex / Q000378:metabolism; D013045:Species Specificity; D013696:Temperature; D000637:Transaminases / Q000378:metabolism
D006600:Hexosephosphates; D011768:Pyruvate Dehydrogenase Complex; D009243:NAD; D009249:NADP; D008291:Malate Dehydrogenase; D007521:Isocitrate Dehydrogenase; D007655:Ketoglutarate Dehydrogenase Complex; D000637:Transaminases; D000446:Aldehyde-Lyases; D008697:Methane
Ob al'ternativnosti puteĭ assimiliatsii metana u obligatnykh metilotrofov