Microsomal N-hydroxylation of trans-4'-halo-4-acetamidostilbenes.
Hamster liver microsomes catalyze the N-hydroxylation of trans-4-acetamidostilbene and its 4'-Cl and 4'-F analogs. Other metabolites which were detected and quantitated were the 4'-OH derivative of trans-4-acetamidostilbene and the glycolamides of the 4'-Cl and 4'-F compounds. The kinetics of metabolism of the acetamidostilbenes were also determined. The apparent KM values of N-hydroxylation of the four substrates were similar, but the maximal velocities showed marked variations. The Vmax for the 4'-Br analog was 16% of that of trans-4-acetamidostilbene, and the rate of N-hydroxylation of the 4'Cl and 4'-F analogs was only 3% of that of the parent compound.
D000818:Animals; D006224:Cricetinae; D006900:Hydroxylation; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D013058:Mass Spectrometry; D008647:Mesocricetus; D008862:Microsomes, Liver / Q000378:metabolism*; D013267:Stilbenes / Q000378:metabolism*; D013997:Time Factors
D013267:Stilbenes