Validation of a simple radiochemical assay measuring hydrolysis of choline-labelled microsomal phosphatidylcholine by phospholipase C. pH-dependence.
Selective hydrolysis of phosphatidylcholine species, which are selectively radioactively labelled in vivo, does not appear to interfere with a radiochemical assay for hydrolysis of microsomal phosphatidylcholine by C-type phospholipases from Bacillus cereus or Clostridium perfringens. Both phospholipases substantially hydrolysed phosphatidylcholine over the pH range 4.0-10.0.
D000818:Animals; D001409:Bacillus cereus / Q000201:enzymology; D003016:Clostridium perfringens / Q000201:enzymology; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008297:Male; D008722:Methods; D008862:Microsomes, Liver / Q000378:metabolism; D010713:Phosphatidylcholines / Q000378:metabolism*; D010740:Phospholipases / Q000378:metabolism*; D051381:Rats
D010713:Phosphatidylcholines; D010740:Phospholipases