Dihydrofolate reductase from amethopterin-resistant Lactobacillus casei. Effects of pH, salts, temperature, and source of NADPH on enzyme activity and substrate specificity studies.
D002021:Buffers; D004352:Drug Resistance, Microbial; D004789:Enzyme Activation; D005493:Folic Acid Antagonists; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007780:Lacticaseibacillus casei / Q000201:enzymology*; D008727:Methotrexate / Q000494:pharmacology*; D008970:Molecular Weight; D009249:NADP / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D009994:Osmolar Concentration; D011487:Protein Conformation; D012492:Salts / Q000494:pharmacology; D013696:Temperature; D013762:Tetrahydrofolate Dehydrogenase / Q000378:metabolism*
D002021:Buffers; D005493:Folic Acid Antagonists; D012492:Salts; D009249:NADP; D013762:Tetrahydrofolate Dehydrogenase; D008727:Methotrexate