Effect of substrate composition and concentration on aortic cholesterol ester hydrolase activity.
Cholesterol ester hydrolase activity of pig aorta has been examined under optimum experimental conditions for hydrolysis of different cholesterol esters. The enzyme specific activity values were in the numerical order of substrates hydrolyzed: cholesteryl linoleate larger than or equal to linolenate greater than palmitate larger than or equal to stearate greater than oleate. The results are discussed in relation to the arterial accumulation of cholesterol esters.
D000818:Animals; D001011:Aorta / Q000201:enzymology*; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D002788:Cholesterol Esters / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008041:Linoleic Acids; D008042:Linolenic Acids; D009829:Oleic Acids; D010168:Palmitates; D013228:Stearates; D002787:Sterol Esterase / Q000378:metabolism*; D013379:Substrate Specificity; D013552:Swine
D002788:Cholesterol Esters; D008041:Linoleic Acids; D008042:Linolenic Acids; D009829:Oleic Acids; D010168:Palmitates; D013228:Stearates; D002265:Carboxylic Ester Hydrolases; D002787:Sterol Esterase