Application of a direct spectrophotometric assay employing a chromogenic substrate for tryptophanase to the determination of pyridoxal and pyridoxamine 5'-phosphates.
D001051:Apoenzymes / Q000302:isolation & purification; D004926:Escherichia coli / Q000201:enzymology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008190:Lyases / Q000302:isolation & purification; D009943:Organophosphorus Compounds / Q000032:analysis; D010770:Phosphotransferases / Q000378:metabolism; D011730:Pyridoxal; D011732:Pyridoxal Phosphate / Q000032:analysis*; D011733:Pyridoxamine / Q000032:analysis*; D012486:Salmonella typhimurium / Q000201:enzymology; D013053:Spectrophotometry / Q000379:methods; D013696:Temperature; D013816:Thermodynamics; D014368:Tryptophanase / Q000302:isolation & purification / Q000378:metabolism
D001051:Apoenzymes; D009943:Organophosphorus Compounds; D011730:Pyridoxal; D011732:Pyridoxal Phosphate; D011733:Pyridoxamine; D010770:Phosphotransferases; D008190:Lyases; D014368:Tryptophanase