Enzymic changes in the cervix of the rat and hamster during the oestrous cycle and the effect of steroids.
Changes in four hydrolytic enzymes, namely acid phosphatase, alkaline phosphatase, arylsulphatase A and B, of the cervix of the rat and hamster have been studied during the 4-day oestrous cycle. All four enzymes showed maximal activity on the day of oestrus and least activity on day 2 of dioestrus. All the enzymes showed significant reduction of activity after ovariectomy, arylsulphatase A and B showing the earliest changes in specific activity. A single subcutaneous injection of 0-02 microng oestradiol-17beta/rat increased the especific activity of arylsulphatase A and B from the low ovariectomized level to that observed in control oestrous animals within 18 and 6 h respectively. A higher concentration of oestradiol 17beta (2-0 microng) had an inhibitory effect. Progesterone was without effect on arylsulphatase B activity, but when given (2-0 mg) with 0-02 microng oestradiol-17beta, it inhibited the response to oestrogen. Cycloheximide prevented the rise in arylsulphatase B activity occurring after oestrogen injection, suggesting a regulation of cervical arylsulphatase B at the level of protein biosynthesis. These results suggest that arylsulphatase B activity may be induced by oestrogen in the cervix of the rat.
D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002369:Castration; D002553:Cerebroside-Sulfatase / Q000378:metabolism; D002584:Cervix Uteri / Q000187:drug effects / Q000201:enzymology*; D002803:Chondro-4-Sulfatase / Q000096:biosynthesis / Q000378:metabolism; D006224:Cricetinae; D003513:Cycloheximide / Q000494:pharmacology; D004790:Enzyme Induction; D004958:Estradiol / Q000494:pharmacology; D004971:Estrus; D005260:Female; D011247:Pregnancy; D011374:Progesterone / Q000494:pharmacology; D051381:Rats
D011374:Progesterone; D004958:Estradiol; D003513:Cycloheximide; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D002553:Cerebroside-Sulfatase; D002803:Chondro-4-Sulfatase