Induction of lipogenesis during differentiation in a "preadipocyte" cell line.
3T3-L1 fibroblasts differentiate in culture into cells having adipocyte character. This transition is accompanied by a 40- to 50-fold  rise in the incorporation of [14C]acetate into triglyceride. The increase in lipogenic rate is exactly parallel to a coordinate rise in the activities of the key enzymes of the fatty acid biosynthetic pathway (ATP-citrate lyase, acetyl-CoA carboxylase, and fatty acid synthetase). Immunological studies indicate that the elevated acetyl-CoA carboxylase activity is the product of an increased cellular enzyme level.
D001275:ATP Citrate (pro-S)-Lyase / Q000378:metabolism; D000085:Acetates / Q000378:metabolism; D000103:Acetyl-CoA Carboxylase / Q000378:metabolism; D000273:Adipose Tissue / Q000378:metabolism*; D002449:Cell Aggregation; D002454:Cell Differentiation; D002460:Cell Line; D064429:Fatty Acid Synthases / Q000378:metabolism; D014280:Triglycerides / Q000096:biosynthesis* / Q000378:metabolism
D000085:Acetates; D014280:Triglycerides; D064429:Fatty Acid Synthases; D001275:ATP Citrate (pro-S)-Lyase; D000103:Acetyl-CoA Carboxylase