[Oxidation of putrescine, spermidine and spermine by diamino-oxidase from mouse liver].
Optimal conditions to determine the activity of diaminooxidase in mouse liver homogenate are described. Maximal oxidation rate for putrescine was found to take place at a concentration of 20 mM and pH 9.5, and for spermidine and spermine--at 10 mM concentration and pH 9.2. The rate of tyramine oxidation was maximal at pH 7.8. Apparent KM values were 4.98-10(-3 M, 1-10(-3) M and 0.8-10(-3) M for putrescine, spermidine and spermine respectively. Hydroxylamine did not inhibit the rate of putrescin oxidation at optimal pH value.
D006631:Amine Oxidase (Copper-Containing) / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D006863:Hydrogen-Ion Concentration; D006898:Hydroxylamines / Q000494:pharmacology; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D051379:Mice; D010084:Oxidation-Reduction; D011073:Polyamines / Q000378:metabolism*; D011700:Putrescine / Q000378:metabolism; D013095:Spermidine / Q000378:metabolism; D013096:Spermine / Q000378:metabolism; D013329:Structure-Activity Relationship; D014439:Tyramine / Q000378:metabolism
D006898:Hydroxylamines; D011073:Polyamines; D013096:Spermine; D006631:Amine Oxidase (Copper-Containing); D013095:Spermidine; D011700:Putrescine; D014439:Tyramine
Okislenie putrestsina, spermidina i spermina diaminooksidazoĭ pecheni mysheĭ.