Effects of divalent cation ionophore A23187 on potassium permeability of rat erythrocytes.
A23187 transports calcium rapidly into rat erythrocytes, apparently by an electroneutral exchange for intracellular magnesium and protons. When red cells are incubated in the absence of any added divalent cations, A23187 transports internal magnesium out of the cells, in exchange for extracellular protons. Magnesium uptake into erythrocytes is produced by A23187, providing the extracellular concentration of this cation exceeds intracellular levels, and the ionophore also transports strontium, but not barium, into red cells. A23187 produces a rapid and extensive loss of intracellular potassium from erythrocytes during uptake of calcium or strontium, but not magnesium. When red cells are incubated in the absence of any exogenous divalent cations, A23187 still produces a potassium efflux and this is inhibited completely by small amounts of ethylene glycol bis(beta-aminoethyl ether)-N,N'-tetraacetic acid and restored by the addition of calcium in excess of the chelator. Although EDTA enhances the extent of magnesium release from erythrocytes incubated with A23187, it prevents the potassium efflux. Dipyridamole and 4-acetamid-4'-isothiocyano-stilbene-2,5'-disulfonic acid, which decrease chloride premeability of erythrocytes, inhibit the A23187-induced potassium loss from red cells. Rutamycin, peliomycin, venturicidin, and A23668B also inhibit potassium efflux from intact cells incubated with A23187, but this effect is not correlated with their abilities to inhibit various ATPases in red cell membrane preparations. It is concluded that A23187 does not transport potassium directly across the erythrocyte plasma membrane, but permits small amounts of endogenous calcium to interact with some membrane component to enhance potassium permeability of the cell.
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001464:Barium / Q000097:blood; D001692:Biological Transport; D001693:Biological Transport, Active; D000001:Calcimycin / Q000494:pharmacology*; D002118:Calcium / Q000097:blood; D002463:Cell Membrane Permeability / Q000187:drug effects; D004492:Edetic Acid / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008274:Magnesium / Q000097:blood; D008297:Male; D011188:Potassium / Q000097:blood*; D051381:Rats; D013324:Strontium / Q000097:blood
D000900:Anti-Bacterial Agents; D001464:Barium; D000001:Calcimycin; D004533:Egtazic Acid; D004492:Edetic Acid; D008274:Magnesium; D011188:Potassium; D002118:Calcium; D013324:Strontium