Diethylaminoethyl-cellulose-bacterial cell immunoadsorbent columns: preparation of serotype-specific globulin and immunofluorescent conjugates for Streptococcus mutans serotypes a and d.
Diethylaminoethyl (DEAE)-cellulose was used as a support material for preparing bacterial cell columns. Pretreatment of the bacterial cells with formalin was essential in obtaining satisfactory adherence of the cells to DEAE-cellulose. Cross-reacting antibodies were removed from antibody preparations against strains of Streptococcus mutans serotypes a and d by adsorption on appropriate bacterial cell columns. S. mutans serotype d was further divided into two subtypes on the basis of immunofluorescent staining with conjugates of immunospecifically adsorbed immunoglobulin G. The DEAE-cellulose-bacterial cell columns were regenerated after use by desorbing the cross-reacting antibodies with low-pH buffer and were used repeatedly over and 18-month period with no detectable loss in effectiveness.
D000327:Adsorption; D002482:Cellulose / Q000031:analogs & derivatives; D003429:Cross Reactions; D003636:DEAE-Cellulose; D005455:Fluorescent Antibody Technique; D007106:Immune Sera / Q000032:analysis; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000378:metabolism*; D012703:Serotyping; D013291:Streptococcus / Q000276:immunology*; D013295:Streptococcus mutans / Q000276:immunology*
D007106:Immune Sera; D007074:Immunoglobulin G; D002482:Cellulose; D003636:DEAE-Cellulose