Synthesis of acyl phosphatidylglycerol from phosphatidylglycerol in Escherichia coli K-12. Evidence for the participation of detergent-resistant phospholipase A and heat-labile membrane-bound factor(s).
The conversion of phosphatidylglycerol to acyl phosphatidylglycerol by extracts of Escherichia coli K-12 strains was examined under various conditions. The maximum rate of conversion was observed at pH 7.2 in the presence of 50% (v/v) diethyl ether and 10 mM CaCl2. This conversion was found to involve two sequential reactions: (1) The formation of 2-acyl glycerophosphoglycerol and 2-acyl glycerophosphoethanolamine from phosphatidylglycerol and phosphatidylethanolamine, respectively, by detergent-resistant phospholipase A in the presence of Ca2+ and (2) transfer of the acyl group of 2-acyl lysophospholipid to phosphatidylglycerol by a heat-labile factor(s) in the presence of diethyl ether. Neither fatty, acid acyl-CoA nor 1-acyl lysophospholipid could act as an acyl donor for phosphatidylglycerol. The heat-labile factor(s) was found in both the inner membrane and supernatant fractions.
D002118:Calcium / Q000494:pharmacology; D002462:Cell Membrane / Q000201:enzymology; D003902:Detergents / Q000494:pharmacology*; D004926:Escherichia coli / Q000378:metabolism*; D004983:Ethanolamines / Q000032:analysis; D005019:Ethyl Ethers; D005227:Fatty Acids / Q000032:analysis; D005994:Glycerophosphates; D006863:Hydrogen-Ion Concentration; D008954:Models, Biological; D010714:Phosphatidylethanolamines; D010715:Phosphatidylglycerols / Q000096:biosynthesis* / Q000378:metabolism; D010740:Phospholipases / Q000378:metabolism*; D010743:Phospholipids / Q000096:biosynthesis; D012997:Solvents; D013696:Temperature
D003902:Detergents; D004983:Ethanolamines; D005019:Ethyl Ethers; D005227:Fatty Acids; D005994:Glycerophosphates; D010714:Phosphatidylethanolamines; D010715:Phosphatidylglycerols; D010743:Phospholipids; D012997:Solvents; C002449:glycerophosphoethanolamine; D010740:Phospholipases; D002118:Calcium