Cultivation of foot and mouth disease virus in bovine leukocyte cultures for use in the complement fixation test.
Bovine leukocyte cultures achieved good growth after 72 hours of cultivation. Foot-and mouth disease (FMD) virus type 0 multiplied in such cultures to a titre of 10(6) TCID50/0.1 ml. The cell culture-grown virus was found to be suitable for the complement fixation test (CFT) after purification and concentration with calcium phosphate.
D000818:Animals; D005537:Aphthovirus / Q000254:growth & development*; D002417:Cattle; D003168:Complement Fixation Tests; D046508:Culture Techniques; D007962:Leukocytes; D014776:Virus Cultivation / Q000379:methods*; D014779:Virus Replication