[Role of the electron acceptor properties of lysozyme and its substrate inhibitors in photosensitized electron transport in their complexes by the EPR method].
The nature of electron-acceptor groups in the system of lysozyme with its substrate-inhibitors has been studied in a wide range of pH values by the method of photosensitized electron transfer. In the lysozyme molecule disulphide bonds and peptide groups are the electron--acceptor groups. The nature of radicals in irradiated lysozyme depends on pH. At complex-formation of lysozyme with oligomeres of N-acetylglucosamine the electron transfer from the enzyme molecule to N-acetyl group of the substrate-inhibitor molecule is realized. Under conditions ruling out complex-formation of lysozyme with the inhibitors (N-acetylglucosamine and its dimer) the electrons are localized on disulphide bonds of the protein molecules at alkaline pH and at pH less than or equal to 3 the radicals are observed which are due to the remove of hydrogen atom from the Calpha-atom of the protein polypeptide chain.
D000117:Acetylglucosamine / Q000528:radiation effects; D055598:Chemical Phenomena; D002621:Chemistry; D004578:Electron Spin Resonance Spectroscopy; D004579:Electron Transport / Q000528:radiation effects; D006863:Hydrogen-Ion Concentration; D009113:Muramidase / Q000037:antagonists & inhibitors / Q000528:radiation effects*; D011108:Polymers; D014466:Ultraviolet Rays
D011108:Polymers; D009113:Muramidase; D000117:Acetylglucosamine
Izuchenie roli élektronoaktseptornykh svoĭstv lizotsima i ego substrat-ingibitorov v fotosensibilizirovannom perenose élektronov v ikh kompleksakh metodom EPR.