Mechanism of action of miconazole: labilization of rat liver lysosomes in vitro by miconazole.
Miconazole, a potent antifungal agent, labilizes rat liver lysosomes. Its labilizing effect is followed by measuring the release of lysosomal hydrolases, namely, acid phosphatase, beta-glucuronidase, and arylsulfatase A. The effect of miconazole is concentration dependent in the range of 10(-5) to 1.2 x 10(-4) M. However, at higher concentrations, miconazole inhibits enzyme release but does not inhibit enzyme activities per se. The effect of miconazole depends on the drug/lysosome ratio and is influenced by the pH of the incubation media, being minimal at alkaline pH. Membrane-active drugs such as nystatin, 2-phenethyl-alcohol, hexachlorophene, and digitonin have been compared with miconazole for their lysosome-labilizing action. The effect of miconazole on the lysosomal membrane is confirmed by a decrease in turbidity of the lysosomal suspension.
D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D006863:Hydrogen-Ion Concentration; D007093:Imidazoles / Q000494:pharmacology*; D066298:In Vitro Techniques; D008099:Liver / Q000648:ultrastructure*; D008247:Lysosomes / Q000187:drug effects*; D008825:Miconazole / Q000494:pharmacology*; D009391:Nephelometry and Turbidimetry; D051381:Rats; D011919:Rats, Inbred Strains; D013997:Time Factors
D007093:Imidazoles; D008825:Miconazole; D000135:Acid Phosphatase