Subsynaptosomal distribution, inhibition, and characterization of the binding of (14C) 5-OH-indole-3-acetaldehyde to brain preparations.
The distribution of the monoamine oxidase (MAO) dependent binding of [14C] serotonin as well as of pre-formed [14C] 5-OH-indole-3-acetaldehyde to synaptic subfractions paralleled the gross distribution of MAO. The binding of [14C] serotonin and MAO activity in brain preparations was inhibited by CNS antidepressants (imipramine) or stimulants (caffeine), by hallucinogens (N,N-dimethyltryptamine), sedatives (chlorpromazine), and other drugs. The protein-lipid nature of the binding macromolecule was determined. The chemical nature of the acceptors was also investigated. Experiments with sodium borohydride indicated the partial formation of imines, those with SH-reactive compounds showed partial involvement of acceptor-SH groups in the binding.
D000319:Adrenergic beta-Antagonists / Q000494:pharmacology; D000818:Animals; D000928:Antidepressive Agents / Q000494:pharmacology; D001665:Binding Sites; D001894:Borohydrides / Q000494:pharmacology; D001921:Brain / Q000378:metabolism*; D002417:Cattle; D006897:Hydroxyindoleacetic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D066298:In Vitro Techniques; D008049:Lipase / Q000494:pharmacology; D008995:Monoamine Oxidase / Q000378:metabolism; D008996:Monoamine Oxidase Inhibitors / Q000494:pharmacology; D004130:N,N-Dimethyltryptamine / Q000494:pharmacology; D010643:Phenoxybenzamine / Q000494:pharmacology; D010740:Phospholipases / Q000494:pharmacology; D051381:Rats; D011955:Receptors, Drug; D012701:Serotonin / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000494:pharmacology; D013570:Synaptic Membranes / Q000378:metabolism; D013574:Synaptosomes / Q000378:metabolism*; D013788:Thalamus / Q000378:metabolism; D014357:Trypsin / Q000494:pharmacology
D000319:Adrenergic beta-Antagonists; D000928:Antidepressive Agents; D001894:Borohydrides; D008996:Monoamine Oxidase Inhibitors; D011955:Receptors, Drug; D013438:Sulfhydryl Compounds; D010643:Phenoxybenzamine; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D008995:Monoamine Oxidase; D010740:Phospholipases; D008049:Lipase; D014357:Trypsin; D004130:N,N-Dimethyltryptamine