A new principle for activity measurement of ADP or ATP dependent enzymes: fluorescence quenching of epsilon-ADP and epsilon-ATP by divalent metal ions.
D000244:Adenosine Diphosphate / Q000031:analogs & derivatives*; D000255:Adenosine Triphosphate / Q000031:analogs & derivatives*; D000469:Alkaline Phosphatase / Q000378:metabolism*; D001665:Binding Sites; D002413:Cations, Divalent; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010732:Phosphofructokinase-1 / Q000378:metabolism*; D011485:Protein Binding; D011755:Pyrophosphatases / Q000378:metabolism*; D011770:Pyruvate Kinase / Q000378:metabolism*; D013050:Spectrometry, Fluorescence / Q000379:methods; D013056:Spectrophotometry, Ultraviolet
D002413:Cations, Divalent; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D010732:Phosphofructokinase-1; D011770:Pyruvate Kinase; D000469:Alkaline Phosphatase; D011755:Pyrophosphatases