[Determination of GSH-DH activity (E.C.1.8.5.1) presence of the enzyme in different wheat varieties (author's transl)].
2,6-dichlorophenol-indophenol is more stable in 75% ethanol than in aqueous solution and ascorbic acid reacts considerably faster in this media than glutathione. The activity determination of glutathione: dehydroascorbic acid oxidoreductase was based on these observations by a photometric measurement of the ascorbic acid resulting from the catalysis. Extracts from various types of wheat were tested and relatively high enzyme activities were found.
D001205:Ascorbic Acid / Q000032:analysis; D002384:Catalysis; D005978:Glutathione / Q000032:analysis; D005980:Glutathione Reductase / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D010783:Photometry; D013045:Species Specificity; D014908:Triticum / Q000032:analysis / Q000201:enzymology*
D007202:Indicators and Reagents; D005980:Glutathione Reductase; D005978:Glutathione; D001205:Ascorbic Acid
Verfahren zur Bestimmung der Aktivität der Glutathion-Dehydrogenase (EC 1.8.5.1). Vorkommen des Enzyms in verschiedenen Weizensorten