Use of cross-linking in studying the structure of RNA tumour viruses.
Treatment of intact avian myeloblastosis virus (AMV) with dimethyl suberimidate dihydrochloride (DMS), a cross-linking agent specific for amino groups, was found to result in progressive cross-linking among viral proteins, as revealed by polyacrylamide gel electrophoresis (PAGE) in the presence of sodium dodecyl sulphate (SDS). Free viral proteins were not cross-linked. The cross-linked protein complex with an apparent molecular weight of 50,000 daltons was studied in detail.
D001354:Avian Leukosis Virus / Q000648:ultrastructure*; D009189:Avian Myeloblastosis Virus / Q000648:ultrastructure*; D004120:Dimethyl Suberimidate / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis; D011487:Protein Conformation; D014764:Viral Proteins / Q000032:analysis*
D010455:Peptides; D014764:Viral Proteins; D004120:Dimethyl Suberimidate