Inhibition by cyanide of drug oxidations in rat liver microsomes.
Cyanide inhibited microsomal activities of aniline hydroxylation and aminopyrine, ethylmorphine and codeine demethylations and produced a modified type II difference spectrum of cytochrome P-450 to give two spectral dissociation constants, 0.21mM and 1.05 mM. The binding of cyanide to cytochrome P-450 resulted in innhibition of NADPH-cytochrome P-450 reductase activity. The cyanide inhibition of drug oxidations was partially avoided by increasing oxygen tension. A possible mechanism for the inhibition of drug oxidations by cyanide is discussed.
D000632:Aminopyrine / Q000378:metabolism; D000814:Aniline Compounds / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D003061:Codeine / Q000378:metabolism; D003486:Cyanides / Q000378:metabolism / Q000494:pharmacology*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D003579:Cytochrome Reductases / Q000378:metabolism; D005036:Ethylmorphine / Q000378:metabolism; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction; D010100:Oxygen / Q000494:pharmacology; D051381:Rats
D000814:Aniline Compounds; D003486:Cyanides; D000632:Aminopyrine; D009249:NADP; D003577:Cytochrome P-450 Enzyme System; D003579:Cytochrome Reductases; D005036:Ethylmorphine; D010100:Oxygen; D003061:Codeine