Purification of aspartate transcarbamylase from Drosophila melanogaster.
A purification procedure is described by which aspartate transcarbamylase was obtained from cultured cells of Drosophila melanogaster as part of a high-molecular-weight enzyme complex. The complex is shown to contain several polypeptides. An antiserum directed against the complex enzyme inhibited in vitro the activity of aspartate transcarbamylase, carbamylphosphate synthetase and dihydro-orotase which were shown to copurify on a sucrose gradient and by gel electrophoresis. A fast preparation procedure using this antiserum yielded a 220 000-molecular-weight protein in addition to the polypeptides present in the complex. A purification procedure is also described to obtain aspartate transcarbamylase from second instar larvae of Drosophila. At this stage, the enzyme is not complexed with carbamylphosphate synthetase and dihydro-orotase but exhibits the same molecular weight as the aspartate transcarbamylase moiety found in the high-molecular-weight complex of cultured cells.
D000818:Animals; D001221:Aspartate Carbamoyltransferase / Q000276:immunology / Q000302:isolation & purification*; D002223:Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing) / Q000302:isolation & purification; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D004080:Dihydroorotase / Q000302:isolation & purification; D004331:Drosophila melanogaster / Q000201:enzymology*; D006882:Hydroxyapatites; D007814:Larva / Q000201:enzymology; D008954:Models, Biological; D008970:Molecular Weight; D010455:Peptides / Q000302:isolation & purification; D011487:Protein Conformation
D006882:Hydroxyapatites; D010455:Peptides; D001221:Aspartate Carbamoyltransferase; D004080:Dihydroorotase; D002223:Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)