The host component of the popliteal lymph node graft-versus-host (GVH) reaction. Identification of cells in the node and kinetics of cell proliferation.
The cellular composition of rat popliteal lymph nodes undergoing a GVH reaction was examined. The great majority of cells isolated from the nodes were lymphocytes, while the contribution of phagocytic cells was negligible (less than 5%). Both T and B cells were present in GVH nodes. However, the proportion of B cells was higher than in normal lymph nodes (40-50% vs 30%). Cells synthesizing DNA were enumerated autoradiographically in sections of GVH nodes up to day 7 of the response. A significant increase in the proportion of 3H thymidine labelled cells had already been found on the third day of the GVH response, before lymph node enlargement was apparent. Of the differnt days tested a maximum of 6% labelled cells was found on day 7. Liquid scintillation counting of cell suspension from such nodes showed an even higher increase in 3H thymidine incorporation in GVH vs normal nodes. These findings indicate that cell proliferation is a central mechanism behind the lymph node enlargement.
D000818:Animals; D001345:Autoradiography; D001402:B-Lymphocytes / Q000276:immunology; D002455:Cell Division; D004247:DNA / Q000096:biosynthesis; D005260:Female; D005455:Fluorescent Antibody Technique; D006087:Graft vs Host Reaction; D007106:Immune Sera; D007958:Leukocyte Count; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D010586:Phagocytes / Q000276:immunology; D051381:Rats; D011919:Rats, Inbred Strains; D013601:T-Lymphocytes / Q000276:immunology
D007106:Immune Sera; D004247:DNA