[Analysis of sheep blood serum haptoglobin].
Three types of haptoglobin (Hp), differing in the number of bands of Hp--Hb complex on polyacrylamide gel electrophoregramm are found in sheep blood serum. HpA, HpB and HpC fractions included one, two-three and six-eight bands respectively. A modified procedure for the HpC isolation is described. Effects of urea, sodium dodecylsulpate, beta-mercaptoethanol, pH and maleinization on the behaviour of HpC under polyacrylamide gel electrophoresis is studied. The data obtained suggest that the HpC molecule consists of two subunits (of alpha and beta types), bound with S--S bounds in an alphabeta-dimer, which form a whole HpC molecule for the expense of non-covalent bonds.
D000818:Animals; D001665:Binding Sites; D006242:Haptoglobins / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D046911:Macromolecular Substances; D008623:Mercaptoethanol; D011485:Protein Binding; D011487:Protein Conformation; D012756:Sheep; D012967:Sodium Dodecyl Sulfate; D014508:Urea
D006242:Haptoglobins; D046911:Macromolecular Substances; D012967:Sodium Dodecyl Sulfate; D008623:Mercaptoethanol; D014508:Urea
Issledovanie gaptoglobina syvorotki krovi obets