Definition of period of induction of deciduoma in the rat using ornithine decarboxylase as a marker of growth onset.
The activity of uterine ornithine decarboxylase (ODC) was measured during the 24 hours after systemic induction of decidualization. Following a latent period of 2.5 hours, activity rose and reached a peak of 5.6 +/- 1.1 fold at 5 hours after induction. The activity then declined and a second, lower peak was seen at 21 hours. The increase in ODC activity was suppressed by treatment with cycloheximide (50 mg/kg) and dactinomycin, 500 microgram/rat, confirming that the enzyme response reflects protein synthesis. The increase and the base-line level of ODC activity were suppressed by ergocornine pretreatment (1 mg/rat) which disturbs the endocrine balance of pseudopregnancy. It was concluded that the growth and differentiation of decidual tissue began 2.5 hours after application of the induction stimulus.
D000818:Animals; D002262:Carboxy-Lyases / Q000378:metabolism*; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D003656:Decidua / Q000187:drug effects*; D005260:Female; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D011247:Pregnancy; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis; D051381:Rats; D013843:Thiazines / Q000494:pharmacology; D013997:Time Factors; D014599:Uterus / Q000201:enzymology*
D013843:Thiazines; D003609:Dactinomycin; D012313:RNA; D003513:Cycloheximide; D002262:Carboxy-Lyases; D009955:Ornithine Decarboxylase