The occurence of a neutral protease and its inhibitor in rat peritoneal macrophages.
The lysate of the glycogen-induced macrophages in rat peritoneal exudate was fractionated by centrifugation and extraction into a water extract, 1 M KCl extract and residue fractions. Approximately 50% of the neutral protease activity toward casein in the lysate was recovered in the KCl extract fraction, which was practically devoid of acid protease, cathepsin D. The pH optimum of the neutral protease toward casein and urea-denatured hemoglobin was pH 8.5. The activity was inhibited strongly by DFP or chymostatin and only partially by HgCl2 or PCMB. Addition of a salt to the reaction medium caused enhancement of the activity with an optimum concentration of 0.25 M: KCl, KBr, KI, NaCl, NaBr, NaI, and MgCl2 were all almost equally effective. When the enzyme preparation was filtered through a column of Sephadex G-75 gel in the presence of 1 M KCl, a larger molecular weight fraction at the void volume was obtained in addition to a smaller molecular weight fraction showing a caseinolytic activity insensitive to KCl concentration. The former was found to have a specific inhibitory effect on the latter activity.
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology*; D002403:Cathepsins / Q000378:metabolism; D006003:Glycogen; D006863:Hydrogen-Ion Concentration; D007531:Isoflurophate / Q000494:pharmacology; D008264:Macrophages / Q000201:enzymology*; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D009994:Osmolar Concentration; D010447:Peptide Hydrolases / Q000302:isolation & purification / Q000378:metabolism*; D011189:Potassium Chloride / Q000494:pharmacology; D011480:Protease Inhibitors; D051381:Rats; D012964:Sodium / Q000494:pharmacology
D011480:Protease Inhibitors; D007531:Isoflurophate; D011189:Potassium Chloride; D006003:Glycogen; D012964:Sodium; D002403:Cathepsins; D010447:Peptide Hydrolases; D008274:Magnesium