Marked difference in electrophoretic migration rates between two influenza A viruses.
Comparative moving boundary electrophoresis revealed that influenza virus A/PR/8/34 (H0N1) has a 2.5 times higher electrophoretic migration rate at pH 7 than influenza virus A/Singpore/1/57 (H2N2). This difference was the same whether the compared viruses were purified first by either ammonium sulphate precipitation or adsorption onto and elution from red blood cells and then by density gradient centrifugation. The same electrophoretic methods was used for testing the homogeneity of influenza virus preparations purified by either method.
D002499:Centrifugation, Density Gradient; D011232:Chemical Precipitation; D004586:Electrophoresis; D009980:Influenza A virus / Q000032:analysis* / Q000302:isolation & purification