Resonance Raman study of the pH-dependent and detergent-induced structural alterations in the heme moiety of Rhodospirillum rubrum cytochrome c'.
The resonance Raman spectra and the structures of the heme moiety of Rhodospirillum rubrum cytochrome c' were investigated for its five states characterized by absorption spectra; Types-a and -n of the reduced form and Types-I, -II, and -III of the oxidized form. The frequency of the ligand-sensitive Raman line suggested the coordination of lysine (Nepsilon) at the sixth position of the heme iron of Type-n. The sixth ligand of Type-III was deduced to be either lysine or histidine but would not be methionine. Type-a and Type-II gave the Raman spectra of rather normal high spin type but Type-I was unusual in the sense that the frequencies of the Raman lines associated primarily with methine-bridge CC-stretching vibrations were relatively high in comparison with those of other high spin hemoproteins. Type-I was converted directly to Type-III upon the addition of SDS or 2-propanol but the conversion occurred via Type-II when pH was increased. Structural difference between the high spin hemes of Type-I and Type-II was discussed in detail.
D003574:Cytochrome c Group; D006418:Heme; D006863:Hydrogen-Ion Concentration; D007501:Iron; D008024:Ligands; D011485:Protein Binding; D011487:Protein Conformation; D012247:Rhodospirillum rubrum / Q000201:enzymology*; D013045:Species Specificity; D013059:Spectrum Analysis, Raman
D003574:Cytochrome c Group; D008024:Ligands; D006418:Heme; D007501:Iron