Determination of acenocoumarol in plasma and urine by double radioisotope derivative analysis.
Acenocoumarol, to which a 14C-labeled internal standard has been added, is extracted at pH 4 into ethyl acetate-heptane (20:80 v/v), back-extracted into aqueous sodium hydroxide solution after solvent washing with a pH 7 buffer, and reextracted after acidification in the solvent mixture. It is then acetylated with 3H-acetic anhydride. The acenocoumarol acetyl derivative is separated from the metabolite derivatives by TLC, and its radioactivity is measured. The method is specific and sensitive to a concentration of 8 ng/ml.
D000074:Acenocoumarol / Q000032:analysis* / Q000097:blood / Q000652:urine; D000107:Acetylation; D002250:Carbon Radioisotopes; D002855:Chromatography, Thin Layer; D006863:Hydrogen-Ion Concentration; D007553:Isotope Labeling; D007700:Kinetics; D008722:Methods; D014316:Tritium
D002250:Carbon Radioisotopes; D014316:Tritium; D000074:Acenocoumarol