Some observations on the NADP+-linked oxidation of methylglyoxal catalysed by 2-Oxoaldehyde dehydrogenase.
In the oxidation of methylglyoxal by 2-oxoaldehyde dehydrogenase, the apparent Km value for NADP+ was about 2.5 times lower than the corresponding Km for NAD+; the apparent Km values for methylglyoxal and for the amine activator L-2-aminopropan-1-ol, with NADP+ as cofactor, were also different from those obtained with NAD+. In the presence of NADP+, the enzyme was not activated by P1, in contrast with the activation of the enzyme when NAD+ was used. The significance of the results is discussed.
D000445:Aldehyde Oxidoreductases / Q000378:metabolism; D000447:Aldehydes / Q000378:metabolism*; D000818:Animals; D007700:Kinetics; D009249:NADP / Q000378:metabolism*; D010084:Oxidation-Reduction; D011765:Pyruvaldehyde / Q000378:metabolism*; D012756:Sheep
D000447:Aldehydes; D009249:NADP; D011765:Pyruvaldehyde; D000445:Aldehyde Oxidoreductases