Correlation of light and electron micrographs of human metaphase chromosomes after incorporation of BUdR and staining with "33258 Hoechst" and Giemsa.
Stained (Giemsa, "33258 Hoechst"1) and "33258 Hoechst" + Giemsa) and unstained metaphase chromosomes from human peripheral lymphocytes, after two rounds of replication in the presence of 5-bromodeoxyuridine (BUdR), have been prepared for electron microscopy. There is a positive correlation between light and electron micrographs. The same differential contrast on electron micrographs has been obtained whether the preparations have been stained or not. We attribute this differential contrast primarily to the lesser condensation of the bifilarly substituted chromatid.
D001973:Bromodeoxyuridine; D002842:Chromatids / Q000502:physiology; D002875:Chromosomes / Q000502:physiology*; D006801:Humans; D007621:Karyotyping; D008214:Lymphocytes / Q000502:physiology; D008854:Microscopy, Electron; D008938:Mitosis; D013194:Staining and Labeling
D001973:Bromodeoxyuridine