Calf lens messenger ribonucleoprotein complexes. Characterization and comparison of template activity with corresponding mRNAs.
Lens messenger ribonucleoprotein complexes have been isolated from calf lens polysomes by sucrose gradient centrifugation after puromycin-induced dissociation. A 10 S mRNA was released from a 13 S messenger ribonucleoprotein complex and a 14 S mRNA from a 19 S messenger ribonucleoprotein complex. Two major protein components with molecular weights of approx. 64 000 and 40 000 were isolated from each of the messenger ribonucleoprotein complexes after RNAase digestion. Buoyant density determinations suggest that the messenger ribonucleoprotein complexes contain approximately one mol of each major protein species per mol mRNA. In contrast to lens mRNA, lens messenger ribonucleoproteins are poor templates for transcription with avian myeloblastosis virus reverse transcriptase. Similar results were also obtained with globin messenger ribonucleoprotein containing either two major protein species (or deficient in the lower molecular weight protein species). Polynucleotide phosphorylase eliminates the reverse transcription template activity of the lens mRNA. This effect is blocked in the messenger ribonucleoprotein. Such observations suggest that at least one of the protein components associated with lens messenger ribonucleoprotein may be located in the 3'-terminal region. Only a small variation in translation activity was observed between the messenger ribonucleoproteins and their respective mRNAs.
D000818:Animals; D009189:Avian Myeloblastosis Virus / Q000201:enzymology; D002417:Cattle; D005914:Globins / Q000096:biosynthesis; D007908:Lens, Crystalline / Q000378:metabolism*; D008970:Molecular Weight; D009698:Nucleoproteins; D011117:Polyribonucleotide Nucleotidyltransferase / Q000378:metabolism; D011485:Protein Binding; D014176:Protein Biosynthesis; D011691:Puromycin; D012333:RNA, Messenger / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D012261:Ribonucleoproteins / Q000378:metabolism; D013698:Templates, Genetic; D014158:Transcription, Genetic
D009698:Nucleoproteins; D012333:RNA, Messenger; D012261:Ribonucleoproteins; D011691:Puromycin; D005914:Globins; D012194:RNA-Directed DNA Polymerase; D011117:Polyribonucleotide Nucleotidyltransferase