Study of the effects of neurotransmitters on the hypothalamus-pituitary-testis function in in vitro cell suspension system.
The effects of different neurotransmitters were tested in vitro on a hypothalamic tissue, collagenase-digested isolated anterior pituitary and Leydig cell suspension system by measuring the testosterone production of the Leydig cells. Neurotransmitters were used in concentrations of 0.25, 1.0, 2.5, 5.0, and 10.0 micrograms/ml incubation medium. Dopamine in doses of 1.0, 2.5, and 5.0 micrograms/ml increased the hypothalamic tissue-induced pituitary-testis activation, while it had no direct effect on pituitary and Leydig cells. Noradrenaline in the concentration range 2.5--10.0 micrograms/ml decreased the luteinizing-hormone-releasing-hormone (LHRH) sensitivity of the pituitary cells. 5.0 and 10.0 micrograms/ml 5-hydroxytryptamine decreased the testosterone production and the hCG sensitivity of the isolated Leydig cells. Carbamylcholine and pilocarpine had no action on the in vitro system at the different levels studied.
D000818:Animals; D002217:Carbachol / Q000494:pharmacology; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D004298:Dopamine / Q000494:pharmacology; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology; D007030:Hypothalamo-Hypophyseal System / Q000187:drug effects / Q000502:physiology*; D007985:Leydig Cells / Q000187:drug effects / Q000502:physiology*; D008297:Male; D018377:Neurotransmitter Agents / Q000494:pharmacology*; D009638:Norepinephrine / Q000494:pharmacology; D010862:Pilocarpine / Q000494:pharmacology; D010903:Pituitary Gland, Anterior / Q000187:drug effects / Q000502:physiology; D012701:Serotonin / Q000494:pharmacology; D013739:Testosterone / Q000378:metabolism
D006063:Chorionic Gonadotropin; D018377:Neurotransmitter Agents; D010862:Pilocarpine; D012701:Serotonin; D007987:Gonadotropin-Releasing Hormone; D013739:Testosterone; D002217:Carbachol; D004298:Dopamine; D009638:Norepinephrine