AKR leukemia-specific surface antigens acquired by malignant transformation: their common and individual specificities.
Antisera against five newly established AKR spontaneous leukemias (KSL) prepared by immunization of KSL cells in (C57B1/6 X C3Hf/He)F1 mice were preabsorbed with non-leukemic AKR lymphoid cells to remove antibodies against virus-associated surface antigens and alloantigens. In absorption tests the antisera showed no cross-reaction by immunofluorescence microscopy with thymocyte, fetal, male-specific H-Y, E and X.1 antigens; with known Gross murine leukemia virus-associated antigens; or with cell surface antigens on Friend, Moloney and Rauscher virus-induced tumors. It was thus shown that the antigens detected were leukemia-specific, were acquired by malignant transformation, and consisted of two types: (1) a common antigen in all the KSL, and (2) individual antigens found in four of the KSL which showed distinct patterns of partial cross-reactivity with the other KSL.
D000042:Absorption; D000818:Animals; D000951:Antigens, Neoplasm; D002462:Cell Membrane / Q000276:immunology*; D002471:Cell Transformation, Neoplastic; D000939:Epitopes; D005260:Female; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D009052:Leukemia Virus, Murine / Q000276:immunology*; D007942:Leukemia, Experimental / Q000276:immunology*; D008297:Male; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation
D000951:Antigens, Neoplasm; D000939:Epitopes; D007106:Immune Sera