Glycerol teichoic acid as an antigenic determinant in a Gram-negative bacterium Butyrivibrio fibrisolvens.
An antigenic determinant isolated from a strain of the Gram-negative bacterium Butyrivibrio fibrisolvens reacted with specific antisera to the polyglycerophosphate backbone of membrane teichoic acids of lactobacilli. It gave a reaction of identity with membrane glycerol lipoteichoic acid and glycerol teichoic acid preparations from lactobacilli, and with phenol extracts of other Gram-positive bacteria. The antigen-antibody reactions was strongly inhibited by glycerol-phosphoryl-glycerol-phosphoryl-glycerol and the chemical composition was consistent with glycerol teichoic acid. It was concluded that this Gram-negative bacterium contained a glycerol teichoic acid whose polyglycerophospate backbone was acting as antigenic determinant. Extracts of 33 out of 52 other strains of butyrivibrios examined gave similar reactions.
D000907:Antibodies, Bacterial; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions / Q000187:drug effects; D000942:Antigens, Bacterial; D001419:Bacteria / Q000032:analysis / Q000276:immunology* / Q000648:ultrastructure; D002462:Cell Membrane / Q000276:immunology; D002473:Cell Wall / Q000276:immunology; D002854:Chromatography, Paper; D000939:Epitopes; D005990:Glycerol; D005994:Glycerophosphates / Q000276:immunology* / Q000494:pharmacology; D005779:Immunodiffusion; D007780:Lacticaseibacillus casei / Q000276:immunology; D011233:Precipitin Tests; D013045:Species Specificity; D013682:Teichoic Acids / Q000032:analysis / Q000276:immunology*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D000939:Epitopes; D005994:Glycerophosphates; D013682:Teichoic Acids; D005990:Glycerol