[Simple method for the preparation of haptoglobin].
The present paper deals with a method permitting the isolation of haptoglobin 2-2 from human serum or plasma. The haptoglobin is adsorbed to DEAE-cellulose at pH 5.1 by batching. The loaded cellulose is given into a column and the haptoglobin eluted by a 0.1 M to 0.15 M acetate buffer. The last step is a gel-chromatography on Sephadex G-200. Disc- and immunoelectrophoresis were used to test the purity. As by-product acid alpha1-glycoprotein can be obtained.
D001798:Blood Proteins / Q000302:isolation & purification; D002848:Chromatography, DEAE-Cellulose / Q000379:methods; D002850:Chromatography, Gel / Q000379:methods; D004589:Electrophoresis, Disc; D006242:Haptoglobins / Q000302:isolation & purification*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007122:Immunoelectrophoresis
D001798:Blood Proteins; D006242:Haptoglobins
Ein einfaches Verfahren zur Präparation von Haptoglobin.