An attempt at isolation and partial characterization of specific antibodies from antithymocyte serum.
Specific anti-T antibody was isolated from rabbit anti-mouse and anti-rat thymocyte antiserum on immunosorbent prepared by fixation of brain homogenate in glutaraldehyde. Conditions of isolation, purity of obtained antibody and cytotoxic activity were followed. The resulting preparation containing immunoglobulin G is contaminated with beta globulin and albumin, contains non-precipitating proteins originating from brain tissue and is cytotoxic for thymocytes. Cytotoxicity for bone marrow cells was not demonstrated.
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D000961:Antilymphocyte Serum / Q000276:immunology*; D001853:Bone Marrow / Q000276:immunology; D001921:Brain / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D006087:Graft vs Host Reaction; D007163:Immunosorbent Techniques; D051379:Mice; D011817:Rabbits; D051381:Rats; D013601:T-Lymphocytes / Q000276:immunology*; D014181:Transplantation Immunology
D000906:Antibodies; D000961:Antilymphocyte Serum