[Enzyme immunoassay for the detection and identification of dengue viruses from infected mosquitoes (author's transl)].
The authors deal with a "double sandwich" enzyme immunoassay for the detection and identification of dengue viruses 1, 2 and 4. They use ground glass-beads (7 mm diameter) coated with human heterospecific anti-dengue antibodies, to bind viruses obtained from infected mosquitoes (Toxorhynchites amboinensis). The mouse antibodies bound to the viruses coated on the beads are revealed by anti-mouse 7S immunoglobulins labelled with peroxydase. The method is practical, sensitive and confident. The standard curves obtained with each type show significant differences from one another until very high dilutions of the viral suspension. Inhibition tests by human immune antibodies and dengue antigens demonstrate the specificity of the reaction. This method is certainly applicable to others arboviruses and allows a large scale epidemiological survey.
D000818:Animals; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D009033:Culicidae / Q000382:microbiology*; D003716:Dengue Virus / Q000145:classification* / Q000276:immunology; D006041:Goats; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G; D051379:Mice; D010544:Peroxidases
D007074:Immunoglobulin G; D010544:Peroxidases
Méthode immunoenzymatique de détection et typage des virus de la dengue à partir de moustiques infectés.