Improvement in specificity of immunofluorescent reagents for identifying Streptococcus mutans by DEAE-cellulose-bacterial cell column immunosorption methods.
We have described a new method for preparing bacterial cell columns, in which DEAE-cellulose is used as the cell support material. The columns can be used to isolate pure antibody or to remove cross-reacting antibodies from immune IgG. The columns are remarkable stable, can be scaled up to any desired dimensions, and should be suitable for large-scale production of highly specific serological reagents. The method is practical for obtaining serotype-specific and polyvalent IF reagents for identifying S. mutans organisms. However, the method could also be used as a tool for sophisticated studies of antigenic relationships that exist among these and other taxonomic groups of microorganisms.
D000907:Antibodies, Bacterial; D002848:Chromatography, DEAE-Cellulose; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D007074:Immunoglobulin G / Q000302:isolation & purification; D007202:Indicators and Reagents; D013045:Species Specificity; D013194:Staining and Labeling; D013291:Streptococcus / Q000276:immunology*; D013295:Streptococcus mutans / Q000145:classification / Q000276:immunology*
D000907:Antibodies, Bacterial; D007106:Immune Sera; D007074:Immunoglobulin G; D007202:Indicators and Reagents