A rapid method for the purification and radioimmunoassay of human alpha-fetoprotein.
Human alpha-fetoprotein (AFP) was isolated from cord serum on an immunoadsorbent column obtained by covalently linking rabbit anti AFP to cyanogen bromide activated Sepharose. Bound AFP was eluted with 8 M urea with better than 50% recovery. The purified AFP was iodinated prior to its use in a double antibody radioimmunoassay. The purification and radioimmunoassay employ commercially available materials. A standard inhibition curve was obtained which allowed determination of AFP levels between 50 and 100 ng/ml in human serum. The assay was verified by measureing AFP levels in normal female serum, pregnancy serum, cord serum, hepatoma ascitic fluid and a standardized AFP solution.
D006528:Carcinoma, Hepatocellular / Q000032:analysis; D003429:Cross Reactions; D005069:Evaluation Studies as Topic; D005260:Female; D005312:Fetal Blood; D005326:Fetal Proteins / Q000097:blood*; D006801:Humans; D008113:Liver Neoplasms; D008832:Microchemistry; D011247:Pregnancy; D011863:Radioimmunoassay / Q000379:methods; D000509:alpha-Fetoproteins / Q000032:analysis / Q000097:blood*
D005326:Fetal Proteins; D000509:alpha-Fetoproteins