A simple and efficient method for preparation of immunoelectrophoretically pure guinea pig IgM and isolation of monospecific anti-IgM antibodies.
This report describes experimental conditions developed for the rapid and efficient preparation of guinea pig IgM in a purified form and in high yield. A combined immunization procedure had to be designed to obtain sufficiently high IgM concentrations in guinea pig serum. The isolation procedure includes precipitation at low pH, followed by sedimentation at 110,000 g and reverse flow gel chromatography on Sephades G-200. Furthermore,we describe in detail the isolation of relatively large amounts of pure and specific anti-guinea pig mu-chain antibodies by immunoaffinity chromatography.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D000917:Antibody Formation; D006168:Guinea Pigs; D006863:Hydrogen-Ion Concentration; D007075:Immunoglobulin M; D007148:Immunoglobulin mu-Chains
D000888:Antibodies, Anti-Idiotypic; D007075:Immunoglobulin M; D007148:Immunoglobulin mu-Chains