Detection of antibody in virus--antibody complexes by immunoferritin labelling and subsequent negative staining.
A technique for the immunoferritin labelling of antibody participating in virus--antibody complexes has been developed. Complexes were labelled by incubation with the immunoferritin reagent and excess reagent removed by polyacrylamide gel electrophoresis. Labelled complexes were then visualised in the electron microscope by negative staining.
D000906:Antibodies / Q000032:analysis*; D000936:Antigen-Antibody Complex; D000956:Antigens, Viral / Q000032:analysis*; D005293:Ferritins / Q000378:metabolism*; D006801:Humans; D009975:Orthomyxoviridae / Q000276:immunology* / Q000648:ultrastructure; D013194:Staining and Labeling
D000906:Antibodies; D000936:Antigen-Antibody Complex; D000956:Antigens, Viral; D005293:Ferritins