Effects of anisomycin and CNS stimulants on brain catecholamine synthesis.
Mice were injected with anisomycin, an inhibitor of cerebral protein synthesis; d-amphetamine, strychnine, or caffeine was administered 30 min later. Fifteen min before sacrifice at 1 or 2 hr after injection of anisomycin, 3H-tyrosine was injected intravenously, and catecholamine synthesis rates were estimated by measurement of the specific activity of 3H-tyrosine and the accumulation of 3H-norepinephrine and 3H-dopamine. Anisomycin decreased synthesis rates of catecholamines, but this effect was not significantly affected by any of the CNS stimulants. These results suggest that the recently reported reversal of anisomycin-induced amnesia by these stimulants is not due to an attenuation of brain catecholamine synthesis inhibition.
D000818:Animals; D000841:Anisomycin / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D002395:Catecholamines / Q000096:biosynthesis*; D000697:Central Nervous System Stimulants / Q000494:pharmacology*; D004298:Dopamine / Q000096:biosynthesis; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009638:Norepinephrine / Q000096:biosynthesis; D011759:Pyrrolidines / Q000494:pharmacology*; D013997:Time Factors; D014443:Tyrosine / Q000378:metabolism
D002395:Catecholamines; D000697:Central Nervous System Stimulants; D011759:Pyrrolidines; D014443:Tyrosine; D000841:Anisomycin; D004298:Dopamine; D009638:Norepinephrine