Studies on the possible identity of particulate beta-glucosidase and beta-xylosidase of mouse liver.
Mouse liver beta-glucosidase (beta-D-glucosidase glucohydrolase, EC 3.2.1.21) and beta-xylosidase (1,4-beta-D-xylan xylohydrolase, EC 3.2.1.37) activities were studied under different conditions of incubation in an attempt to determine whether these two activities are due to a single enzyme or two separate enzymes. The results showed that: (a) Particle-bound beta-glucosidase and beta-xylosidase activities exhibit similar characteristics with different buffers and at various pH values, in the presence or absence of taurocholate. (b) Both activities are inhibited by gluconolactone and conduritol B eposice. beta-Glucosidase activity is inhibited competitively by the two inhibitors, but beta-xylosidase activity is inhibited non-competitively. (c) Xylonolactone was a very poor inhibitor of both activities, but the inhibition of beta-xylosidase activity was more pronounced than that of beta-glucosidase. (d) The presence of glucosides or xylosides simultaneously in the incubation medium suggested the presence of one enzyme with both activities. These results, together with the mode of inhibition produced by gluconolactone and conduritol B epoxide also suggest the presence of two different binding sites for the beta-D-glucoside and beta-D-xyloside, respectively.
D000818:Animals; D005942:Gluconates / Q000494:pharmacology; D005959:Glucosidases / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007294:Inositol / Q000031:analogs & derivatives / Q000494:pharmacology; D007700:Kinetics; D007783:Lactones / Q000494:pharmacology; D008099:Liver / Q000201:enzymology*; D051379:Mice; D013656:Taurocholic Acid / Q000494:pharmacology; D014995:Xylosidases / Q000378:metabolism*; D001617:beta-Glucosidase / Q000378:metabolism*
D005942:Gluconates; D007783:Lactones; D007294:Inositol; D013656:Taurocholic Acid; D005959:Glucosidases; D006026:Glycoside Hydrolases; D014995:Xylosidases; D001617:beta-Glucosidase; C011423:conduritol epoxide; C010730:beta-glucono-1,5-lactone