Oncornavirus-like particles released from arginine-deprived human lymphoblastoid cell lines.
Type-C RNA tumor virus particles were released from three different human lymphoblastoid cell lines after incubation in arginine-deficient medium. The released virus-like particles were characterized by (a) their ability to band in sucrose gradients at a density of 1.16-1.18 g/ml; (b) the presence of an RNA-directed DNA polymerase activity resembling that of the oncornaviruses; and (c) isolation of cores that band at a density of 1.26-1.27 g/ml in sucrose gradients. Examination of the arginine-deprived human lymphoblastoid cell line strain P3HR-1 by electron microscopy revealed the presence of C-type particles in the intracellular spaces.
D001120:Arginine / Q000378:metabolism*; D002051:Burkitt Lymphoma / Q000382:microbiology; D002460:Cell Line; D004279:DNA, Viral / Q000096:biosynthesis; D007700:Kinetics; D007938:Leukemia / Q000382:microbiology; D009693:Nucleic Acid Hybridization; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D012190:Retroviridae / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure
D004279:DNA, Viral; D001120:Arginine; D012194:RNA-Directed DNA Polymerase