[Study of human secretory immunoglobulin A. I. Obtaining monospecific antiserum to human secretory immunoglobulin A].
A method of obtaining monospecific antiserum to the human secretory IgA is described. Immunochemically pure secretory IgA (isolated from human colostrum by fractionation with ammonium sulfate and gel-filtration on Sephadex G-200) was used for immunization of rabbits or sheep. Heterologous antibodies were removed by adsorption with commercial gamma globulin, normal serum, the serum of a patient suffering from A-myeloma with the IgA polymere and purified lactoferrin. Monospecific antiserum to the secretory IgA gave a reaction of complete immunological identity with the secretory IgA and a free secretory component.
D000327:Adsorption; D000818:Animals; D000910:Antibodies, Heterophile / Q000302:isolation & purification; D000941:Antigens / Q000008:administration & dosage; D003126:Colostrum / Q000276:immunology; D000939:Epitopes; D005260:Female; D006801:Humans; D007106:Immune Sera; D007114:Immunization; D007122:Immunoelectrophoresis; D007070:Immunoglobulin A / Q000302:isolation & purification*; D007071:Immunoglobulin A, Secretory / Q000302:isolation & purification*; D007158:Immunologic Techniques; D007781:Lactoferrin / Q000302:isolation & purification; D009031:Moscow; D011817:Rabbits; D012756:Sheep
D000910:Antibodies, Heterophile; D000941:Antigens; D000939:Epitopes; D007106:Immune Sera; D007070:Immunoglobulin A; D007071:Immunoglobulin A, Secretory; D007781:Lactoferrin
Izuchenie sekretornogo immunoglobulina A cheloveka. Soobshchenie I. Poluchenie monospetsificheskoĭ antisyvorotki k sekretornomu immunoglobulinu A cheloveka