Effect of cytosine arabinoside on viral-specific protein synthesis in cells infected with herpes simplex virus.
The relationship between viral DNA and protein synthesis during herpes simplex virus type 1 (HSV-1) replication in HeLa cells was examined. Treatment of infected cells with cytosine arabinoside (ara-C), which inhibited the synthesis of HSV-1 DNA beyond the level of detection, markedly affected the types and amounts of viral proteins made in the infected cell. Although early HSV-1 proteins were synthesized normally, there was a rapid decline in total viral protein synthesis beginning 3 to 4 h after infection. This is the time that viral DNA synthesis would normally have been initiated. ara-C also prevented the normal shift from early to late viral protein synthesis. Finally, it was shown that the effect of ara-C on late protein synthesis was dependent upon the time after infection that the drug was added. These results suggest that inhibition of progeny viral DNA synthesis by ara-C prevents the "turning on" of late HSV-1 protein synthesis but allows early translation to be "switched off."
D001120:Arginine / Q000378:metabolism; D003561:Cytarabine / Q000494:pharmacology; D004279:DNA, Viral / Q000096:biosynthesis; D003609:Dactinomycin / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D008239:Lysine / Q000378:metabolism; D014176:Protein Biosynthesis; D018139:Simplexvirus / Q000254:growth & development / Q000378:metabolism*; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014764:Viral Proteins / Q000096:biosynthesis*; D014779:Virus Replication
D004279:DNA, Viral; D014764:Viral Proteins; D003561:Cytarabine; D014316:Tritium; D003609:Dactinomycin; D001120:Arginine; D008239:Lysine; D013936:Thymidine