Electrophoretic separation, detection, and variation of amylase isoenzymes.
An electrophoretic technique for demonstrating amylase isoenzymes is described. After separation in an agarose gel containing a linear polyacrylamide polymer to reduce electroendosmotic flow, the amylase fractions are visualized by incubation with a commercially available dye-starch polymer (Phadebas Amylase Test). The technique detects amylase fractions with activities below 10 U/l. Some characteristic changes in such diseases as acute and chronic pancreatitis, cystic fibrosis of the pancreas, macroamylasemia and inherited variants as well as after maxillofacial surgery are mentioned.
D000208:Acute Disease; D000681:Amylases / Q000032:analysis* / Q000097:blood; D002908:Chronic Disease; D003550:Cystic Fibrosis / Q000201:enzymology; D004587:Electrophoresis, Agar Gel; D006801:Humans; D007527:Isoenzymes / Q000032:analysis*; D010195:Pancreatitis / Q000201:enzymology; D012463:Saliva / Q000201:enzymology; D013194:Staining and Labeling
D007527:Isoenzymes; D000681:Amylases