Mammalian and microbial cell-free conversion of anthracycline antibiotics and analogs.
Cell-free preparations of Streptomyces nogalater and rat liver catalyze reduced pyridine nucleotide dependent conversion of nogalamycin to 7-deoxynogalarol and nogalose (Scheme 1). The mammalian process requires TPNH and has a specific activity of 85 nmoles of 7-deoxynogalarol formed per hour per mg of protein while the bacterial process prefers DPNH and has a specific activity of 5. The oxygen-sensitive conversions have pH optima of 7.5 (rat) and 9 (S. nogalater). Other anthracycline substrates converted to their 7-deoxyaglycones by both systems include nogamycin, 7(R)-O-methylnogarol, 7(R)-O-methylnogalarol, doxorubicin (Adriamycin), steffimycin, and steffimycin B.
D000818:Animals; D000873:Anthracenes / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D002474:Cell-Free System; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D051381:Rats; D013302:Streptomyces / Q000378:metabolism; D013997:Time Factors
D000873:Anthracenes; D000900:Anti-Bacterial Agents; D011506:Proteins