Inhibition of creatine kinase by iodoalkanes. Further appraisal of the essential nature of the reactive thiol group.
Creating kinase (ATP: creatine N-phosphotransferase) is completely inhibited by low molecular weight iodoalkanes in a pseudo first order reaction. Analysis of this and other data suggests that covalent modification per se is not a sufficient criterion to establish whether or not an enzyme group is essential for catalysis.
D000473:Alkanes / Q000494:pharmacology; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D003402:Creatine Kinase / Q000037:antagonists & inhibitors*; D006847:Hydrocarbons, Iodinated / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D011817:Rabbits; D013379:Substrate Specificity; D013438:Sulfhydryl Compounds
D000473:Alkanes; D006847:Hydrocarbons, Iodinated; D013438:Sulfhydryl Compounds; D003402:Creatine Kinase