Metabolism of 4-hydroxy-2,3-pentene-1-al in submitochondrial fractions of mouse liver.
The location of the enzymic system involved in the metabolism of 4-hydroxy-2-3-pentene-1-al (HPE) in the livers of female mice (CBA/CA) has been established. All mitochondrial membranes showed some activity with slightly higher metabolism in the matrix. The enzymic system had a pH optimum of 8.8 using pyrophosphate buffer. Suitable marker enzymes were used to test the contamination of the different submitochondrial fractions with each other.
D000447:Aldehydes / Q000378:metabolism*; D000466:Alkadienes / Q000378:metabolism*; D000818:Animals; D005260:Female; D006863:Hydrogen-Ion Concentration; D007425:Intracellular Membranes / Q000201:enzymology; D008099:Liver / Q000201:enzymology* / Q000648:ultrastructure; D051379:Mice; D008930:Mitochondria, Liver / Q000032:analysis / Q000378:metabolism*
D000447:Aldehydes; D000466:Alkadienes