Observations on the pyridoxal 5'-phosphate inhibition of DNA polymerases.
Pyridoxal 5'-phosphate at concentrations greater than 0.5 mM inhibits polymerization of deoxynucleoside triphosphate catalyzed by a variety of DNA polymerases. The requirement for a phosphate as well as aldehyde moiety of pyridoxal phosphate for inhibition to occur is clearly shown by the fact that neither pyridoxal nor pyridoxamine phosphate are effective inhibitors. Since the addition of nonenzyme protein or increasing the amount of template primer exerted no protective effect, there appears to be specific affinity between pyridoxal phosphate and polymerase protein. The deoxynucleoside triphosphates, however, could reverse the inhibition. The binding of pyridoxal 5'-phosphate to enzyme appears to be mediated through classical Schiff base formation between the pyridoxal phosphate and the free amino group(s) present at the active site of the polymerase protein. Kinetic studies indicate that inhibition by pyridoxal phosphate is competitive with respect to substrate deoxynucleoside triphosphate(s).
D000818:Animals; D009189:Avian Myeloblastosis Virus / Q000201:enzymology; D001665:Binding Sites; D004254:DNA Nucleotidyltransferases / Q000037:antagonists & inhibitors*; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D011485:Protein Binding; D011732:Pyridoxal Phosphate / Q000494:pharmacology*; D051381:Rats; D011925:Rauscher Virus / Q000201:enzymology; D018894:Reverse Transcriptase Inhibitors; D013045:Species Specificity; D013329:Structure-Activity Relationship; D013698:Templates, Genetic
D018894:Reverse Transcriptase Inhibitors; D008345:Manganese; D011732:Pyridoxal Phosphate; D004254:DNA Nucleotidyltransferases; D008274:Magnesium