Use of the analytical electron microscope (AEM) in cytochemical studies of the central nervous system.
Central nervous tissues (median eminence and arcuate nucleus) were studied by means of energy dispersive x-ray analysis using electron optical systems (analytical electron microscopy). These studies were conducted after the tissue had been treated specifically for localized biogenic amines (BA). The results indicate that not only is the BA cytochemical reaction highly specific, and that BAs can be localized intraneuronally in areas not previously identified, but also that the analytical electron microscope is a very valuable and potentially powerful tool in the studies of inclusion bodies and organelles in the central nervous system and other tissues. Thus, the nonspecific density production by osmium tetroxide can be elucidated from the specific BA reaction plus other areas of the nervous system containing density producing heavy metals, i.e., iron are readily identifiable.
D000313:Adrenal Medulla / Q000032:analysis; D000818:Animals; D001679:Biogenic Amines / Q000032:analysis*; D002415:Cats; D002857:Chromium; D000882:Haplorhini; D007030:Hypothalamo-Hypophyseal System / Q000032:analysis*; D008253:Macaca mulatta; D008473:Median Eminence / Q000032:analysis*; D008854:Microscopy, Electron; D009992:Osmium; D013194:Staining and Labeling
D001679:Biogenic Amines; D002857:Chromium; D009992:Osmium