Allogeneic radiation chimeras. Long-term studies.
Lethally irradiated mice protected with allogeneic fetal liver cells or with syngeneic or allogeneic marrow and spleen cells treated with antisera to mouse immunoglobulins or to the T cell-associated theta antigen and their controls were observed for up to 750 days. The best survival rates were found in the large groups given syngeneic marrow and spleen or allogeneic fetal liver cells (70-85% 700-day survival); in contrast, 43% of the group injected with allogeneic cells treated with anti-theta serum and 19% of those given antiimmunoglobulin-treated cells were alive 700 days postradiation. Pulmonary infection was the most frequent cause of death of long-term survivors in all groups. Tumor incidence was increased in recipients of allogeneic cells (13% versus 4% among syngeneic chimeras), but the renal pathology seen in these groups was no greater than that noted in the syngeneic controls. Beginning 600 days after irradiation, mice from experimental and control groups were killed and their spleens were cultured with thymus-dependent antigens and the mitogens concanavalin A and lipopolysaccharide, Escherichia coli. The most frequent finding in all groups was mild to moderate impairment of T cell-dependent responses.
D000818:Animals; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D003208:Concanavalin A; D004140:Dinitrophenols / Q000276:immunology; D004622:Embryo, Mammalian; D004926:Escherichia coli / Q000276:immunology; D005333:Fetus; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D008070:Lipopolysaccharides; D008099:Liver / Q000166:cytology; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D017710:Lymphocyte Transfusion; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D011135:Polysaccharides, Bacterial; D011507:Proteinuria / Q000175:diagnosis; D011828:Radiation Chimera; D013154:Spleen / Q000166:cytology / Q000276:immunology; D014184:Transplantation, Homologous; D014185:Transplantation, Isogeneic; D005719:gamma-Globulins
D004140:Dinitrophenols; D007106:Immune Sera; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D005719:gamma-Globulins; D003208:Concanavalin A