Presence of "receptors" for growth hormone on membranes of rat thymocytes.
Growth hormone conjugated to Sepharose-4 B was found to mimic the action of the free hormone in the stimulation of the uptake and incorporation of radioactive uridine into RNA in thymocytes in vitro.  The integrity of the cellular structure was essential for the stimulating effect of the hormone.  The bonding of the immunologically reactive determinants of the hormone on membranes of thymocytes was demonstrated by electron micrographs of preparations incubated sequentially with rabbit antigrowth hormone globulins and monospecific sheep anti-rabbit globulins tagged with horse-radish peroxidase.
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D000939:Epitopes; D013006:Growth Hormone / Q000276:immunology / Q000378:metabolism*; D066298:In Vitro Techniques; D008928:Mitochondria / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D011817:Rabbits / Q000276:immunology; D051381:Rats; D011956:Receptors, Cell Surface; D012756:Sheep / Q000276:immunology; D013268:Stimulation, Chemical; D013950:Thymus Gland / Q000166:cytology / Q000378:metabolism*; D014529:Uridine / Q000378:metabolism
D000939:Epitopes; D011956:Receptors, Cell Surface; D012313:RNA; D013006:Growth Hormone; D014529:Uridine