[Comparative biochemical studies of polyhedral proteins of nuclear polyhedrosis viruses].
Using disc polyacrylamide gel electrophoresis, the molecular weights of polyhedral proteins of nuclear polyhedrosis viruses (NPV) of Porthetria dispar, Mamestra brassicae, and Aporia crataegi were found to be 28000 +/- 3000. It was shown that NPV polyhedra of Bombyx mori, Galleria mellonella, P. dispar, and M. brassicae contain a protease. During dissolution of the polyhedra at pH 10,5 this protease specifically cleaves the matrix protein into 2--5 fragments. The amino acid compositions of NPV polyhedral proteins of P. dispar, M. brassicae, A. crataegi, Hyphantria cunae were shown to be very similar. It was found that tyrosine is a C-terminal amino acid of NPV polyhedral proteins of P. dispar, M. brassicae, and A. crataegi.
D006801:Humans; D006863:Hydrogen-Ion Concentration; D007304:Insect Viruses / Q000032:analysis*; D007313:Insecta; D046911:Macromolecular Substances; D008970:Molecular Weight; D013045:Species Specificity; D014764:Viral Proteins / Q000302:isolation & purification
D046911:Macromolecular Substances; D014764:Viral Proteins
Sravnitel'noe binokhimicheskoe issledovanie poliedrennykh belkov virusov iadernogo poliedroza.