title: [Evaluation of cytochemical methods of studying the localization of alkaline phosphatase in B. subtilis].
abstract: A study was made of a possibility of using the "lead" and the "calcium" methods for the determination of localization of alkaline phosphatase in the bacterial cells. Cytochemical medium containing lead nitrate could not be used for determination of the true localization of the alkaline phosphatase in the microorganisms.
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis*; D001412:Bacillus subtilis / Q000201:enzymology*; D002118:Calcium; D003470:Culture Media; D006651:Histocytochemistry / Q000379:methods*; D007854:Lead; D008854:Microscopy, Electron; D009566:Nitrates; D013194:Staining and Labeling; D013696:Temperature; D013997:Time Factors
chemical_list: D003470:Culture Media; D009566:Nitrates; D007854:Lead; D000469:Alkaline Phosphatase; D002118:Calcium
vernacular_title: Otsenka tsitokhimicheskikh metodov izucheniia lokalizatsii shchelochnoĭ fosfatazy u B. subtilis