Antigenicity of methylated deoxyribonucleic acid.
Methylated salmon sperm deoxyrinbonucleic acid (Me-ssDNA) prepared by using dimethyl sulfate was used to elicit antibodies in rabbits. The antisera obtained reacted specifically with Me-ssDNA but not with thermally denatured Me-DNA, native DNA, thermally denatured DNA or ribonucleic acids (RNAs). Antibody activity was demonstrable in both IgG and IgM fractions which were obtained by Sephadex G-200 column chromatography. A single precipitating line was formed by either of IgG and IgM fractions in the double diffusion test with Me-ssDNA.
D000818:Animals; D000918:Antibody Specificity; D000941:Antigens; D004247:DNA / Q000276:immunology*; D000939:Epitopes; D007074:Immunoglobulin G / Q000302:isolation & purification; D007075:Immunoglobulin M / Q000302:isolation & purification; D008745:Methylation
D000941:Antigens; D000939:Epitopes; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D004247:DNA