Alkali-stable oligosaccharides isolated by hydrazinolysis of M and N blood group glycoproteins.
Three alkali-stable oligosaccharides free of amino acids were purified from hydrazinolysis products of M or N blood group glycoprotein. These three oligosaccharides differed in the content of sialic acid and contained similar amounts of other carbohydrate components. The asparagine-linked oligosaccharides, obtained by strong alkaline degradation of M glycoprotein, showed similar electrophoretic mobilities and chemical composition to the oligosaccharides obtained by hydrazinolysis. It indicates that hydrazinolysis does not give a substantial degradation of carbohydrates of alkali-stable oligosaccharides of human red cell glycoprotein.
D055598:Chemical Phenomena; D002621:Chemistry; D006023:Glycoproteins / Q000097:blood*; D006601:Hexoses / Q000097:blood; D006801:Humans; D006834:Hydrazines; D006863:Hydrogen-Ion Concentration; D008951:MNSs Blood-Group System; D009844:Oligosaccharides / Q000097:blood; D012794:Sialic Acids / Q000097:blood
D006023:Glycoproteins; D006601:Hexoses; D006834:Hydrazines; D008951:MNSs Blood-Group System; D009844:Oligosaccharides; D012794:Sialic Acids