Human serum dopamine beta-hydroxylase: correlation of enzymatic activity with immunoreactive protein in genetically defined samples.
An antibody against human adrenal dopamine beta-hydroxylase (DBH) was used to quantitate immunoreactive DBH protein in human serum by an immunoprecipitation technique. A significant correlation was found between DBH enzyme activity and immunoreactive DBH protein in randomly selected serum samples (r = 0.94; N = 38; p less than .001). Studies of sera from obligate heterozygotes and individuals homozygous for the allele responsible for very low serum DBH enzymatic activity were compatible with a genetically mediated decrease in the quantity of circulating DBH protein in these subjects.
D000311:Adrenal Glands / Q000276:immunology; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D004299:Dopamine beta-Hydroxylase / Q000097:blood* / Q000276:immunology; D000939:Epitopes; D005838:Genotype; D006801:Humans; D008722:Methods
D000939:Epitopes; D004299:Dopamine beta-Hydroxylase