title: Selective loss of noradrenergic phenotypic characters in neuroblasts of the rat embryo.
abstract: To define the fate of embryonic neuroblasts in rat gut, which transiently express several noradrenergic traits, we investigated the high-affinity uptake of norepinephrine. At 12.5 days of gestation, these cells exhibited immunoreactivity to tyrosine hydroxylase [tyrosine 3-monoxygenase; L-tyrosine, tetrahydropteridine:oxygen oxidoreductase (3-hydroxylating), EC 1.14.16.2] and endogenous catecholamine fluorescence. However, by 13.5 days these noradrenergic neurotransmitter phenotypic characters essentially disappeared. In contrast, norepinephrine uptake, which was also apparent at 12.5 days, persisted at least through 17.5 days. These observations indicate that norepinephrine uptake develops as an additional noradrenergic characteristic in these cells and persists after the disappearance of other noradrenergic traits. Consequently, neurotransmitter phenotypic characters may be transiently displayed during normal development in vivo.
mesh_terms: D000320:Adrenergic Fibers / Q000196:embryology* / Q000378:metabolism; D000818:Animals; D004064:Digestive System / Q000196:embryology / Q000294:innervation*; D005455:Fluorescent Antibody Technique; D005865:Gestational Age; D009638:Norepinephrine / Q000378:metabolism*; D010641:Phenotype; D051381:Rats; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D014446:Tyrosine 3-Monooxygenase; D009638:Norepinephrine