Preparation and some properties of highly purified human serum kallikrein.
A 3000--6000-fold purified kallikrein was obtained from human serum in 10--25% yield by chromatography on QAE-Sephadex A-50, Molselect CM-50 and on soybean trypsin inhibitor (SBTI)-AH-Sepharose 4-B. The enzyme had a specific activity of 14--23 U, as measured by BAEE hydrolysis. Some properties of highly purified kallikrein are described.
D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D004800:Enzymes, Immobilized; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007610:Kallikreins / Q000097:blood / Q000302:isolation & purification*; D007700:Kinetics; D013696:Temperature; D014360:Trypsin Inhibitor, Kunitz Soybean
D004800:Enzymes, Immobilized; D014360:Trypsin Inhibitor, Kunitz Soybean; D007610:Kallikreins