Mechanism of S-(carboxymethylthio)cysteine cleavage by rat liver cystathionine-gamma-lyase.
Rat liver cystathionine-gamma-lyase [L-cystathionine cysteinelyase (deaminating), EC 4.4.1.1] catalyzes the formation of pyruvic acid, ammonia, and carboxymethylhydrodisulfide from S-(carboxymethylthio)cysteine (CMTC). As judged by pyruvic acid production, the optimal pH is 8.3 in tris-HCl buffer and the Km is 2.9 mM. A possible mechanism of CMTC cleavage by cystathionase is proposed.
D000818:Animals; D002233:Carbocysteine / Q000031:analogs & derivatives* / Q000378:metabolism; D003542:Cystathionine gamma-Lyase / Q000378:metabolism*; D003545:Cysteine / Q000031:analogs & derivatives* / Q000378:metabolism; D006862:Hydrogen Sulfide / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008099:Liver / Q000201:enzymology*; D008190:Lyases / Q000378:metabolism*; D011773:Pyruvates / Q000378:metabolism; D051381:Rats
D011773:Pyruvates; D002233:Carbocysteine; D008190:Lyases; D003542:Cystathionine gamma-Lyase; D003545:Cysteine; D006862:Hydrogen Sulfide