Clinical investigation of serum deoxyribonuclease: I. Analysis of serum deoxyribonuclease activity in comparison with normal and after endoscopic retrograde pancreatography.
Serum Deoxyribonuclease (DNase) micro-assay method was developed using 32P-labelled E. coli DNA as substrate. The serum DNase showed maximum activity at pH 7.5. It required Mg+ for activity, and was inhibited by EDTA or EGTA. The enzyme was also inhibited by actin (60-65%) or bovine pancreatic DNase I antibody (40-45%). The serum DNase activity was markedly increased following endoscopic retrograde pancreatography (ERP) examination. These results imply that serum DNase activity is mostly at least 60-65% pancreatic DNase I.
D000199:Actins / Q000494:pharmacology; D000681:Amylases / Q000097:blood; D000906:Antibodies; D003851:Deoxyribonucleases / Q000097:blood* / Q000276:immunology; D004492:Edetic Acid / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D004724:Endoscopy; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000494:pharmacology; D008722:Methods; D010179:Pancreas / Q000000981:diagnostic imaging* / Q000201:enzymology; D010195:Pancreatitis / Q000000981:diagnostic imaging / Q000201:enzymology; D011859:Radiography
D000199:Actins; D000906:Antibodies; D004533:Egtazic Acid; D004492:Edetic Acid; D003851:Deoxyribonucleases; D000681:Amylases; D008274:Magnesium