A microprecipitation test for rapid detection and identification of Venezuelan, eastern and western equine encephalomyelitis viruses.
The development of a new diagnostic procedure for the identification of Venezvelan, eastern and western equine encephalomyelitis (VEE, EEE, WEE) viruses is described. The procedure utilizes virus precipitation with reference fluorescein-conjugated gamma globulin, followed by cellulose acetate electrophoresis. Clinical specimens containing varying concentrations of virus yielded, in primary duck embryo cell culture, sufficient virus for detection within 22 to 44 hours. Identification of VEE, EEE and WEE virus in specimens was accomplished by microprecipitation within this time. In contrast to conventional identification methods, our procedure eliminates the cost of utilizing laboratory animals and considerably reduces the time required for virus identification.
D000818:Animals; D000914:Antibodies, Viral; D000956:Antigens, Viral; D002478:Cells, Cultured; D011232:Chemical Precipitation / Q000379:methods*; D003168:Complement Fixation Tests; D004372:Ducks; D004586:Electrophoresis; D004622:Embryo, Mammalian; D004625:Embryo, Nonmammalian; D004666:Encephalitis Virus, Venezuelan Equine / Q000302:isolation & purification*; D004667:Encephalitis Virus, Western Equine / Q000302:isolation & purification*; D004668:Encephalitis Viruses / Q000302:isolation & purification*; D004683:Encephalomyelitis, Equine / Q000382:microbiology*; D005452:Fluoresceins; D006385:Hemagglutination Inhibition Tests; D007106:Immune Sera; D011817:Rabbits / Q000276:immunology; D013997:Time Factors; D010948:Viral Plaque Assay; D014776:Virus Cultivation; D005719:gamma-Globulins
D000914:Antibodies, Viral; D000956:Antigens, Viral; D005452:Fluoresceins; D007106:Immune Sera; D005719:gamma-Globulins