The differential binding of antibody from the sera of patients with pemphigus and pemphigoid to isolated guinea-pig epidermal cells.
A method for preparing isolated epidermal cells using trypsin is described. The isolated cells retain the ability to bind antibody from patients with pemphigus or with pemphigoid in a disease-specific.
D000818:Animals; D001485:Basement Membrane / Q000276:immunology; D001666:Binding Sites, Antibody; D002469:Cell Separation / Q000379:methods; D003937:Diagnosis, Differential; D004423:Ear; D000939:Epitopes; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D066298:In Vitro Techniques; D010392:Pemphigus / Q000175:diagnosis / Q000276:immunology*; D012867:Skin / Q000276:immunology; D012872:Skin Diseases, Vesiculobullous / Q000175:diagnosis / Q000276:immunology*; D014357:Trypsin
D000939:Epitopes; D014357:Trypsin