[Properties of immobilized complexes of Streptomyces griseus proteases on different carriers].
A complex of Str. griseus proteases was obtained. The proteases were immobilized on Sephadex G-200 and agarose by the bromo-cyanogen method and on the microcrystalline cellulose by means of TiCl3. The enzymic complex immobilized on different carriers possesses the proteolytic activity of a wide specificity. Due to addition to different carriers the activity of certain enzymes producing the complex varies. The immobilized complex is shown to be more stable in the alkaline zone. The immobilized complex is shown to be more stable in the alkaline zone. The temperature optimum of the activity of the immobilized forms is higher than in the initial enzyme and is 70--75 degrees C. Under multiple application of the immobilized forms of the protease complex the preparation obtained on Sephadix G-200 by the bromo-cyanogen method is most stable: it retains 70% of initial activity after 10-repeated applications.
D003488:Cyanogen Bromide; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008722:Methods; D009097:Multienzyme Complexes / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism*; D013305:Streptomyces griseus / Q000201:enzymology*; D013379:Substrate Specificity; D013696:Temperature
D004800:Enzymes, Immobilized; D009097:Multienzyme Complexes; D010447:Peptide Hydrolases; D003488:Cyanogen Bromide
Svoĭstva immobilizovannykh kompleksov proteaz Streptomyces Griseus na razlichnykh nositeliakh.