Relationship between the ability of nicotinamide to maintain nicotinamide-adenine dinucleotide in rat liver cell culture and its effect on cytochrome P-450.
Rat hepatocytes cultured for 24 h lose 60% of their NAD content. Treatment with nicotinamide prevents the loss of NAD as well as the previously reported loss of cytochrome P-450, suggesting a possible causal relationship. However, isonicotinamide also prevents the loss of cytochrome P-450, but does not increase the concentration of NAD, demonstrating that the ability of nicotinamide to maintain cytochrome P-450 is not apparently related to its effect on the NAD content of cultured hepatocytes.
D000818:Animals; D002478:Cells, Cultured; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009243:NAD / Q000378:metabolism*; D009249:NADP / Q000378:metabolism; D009536:Niacinamide / Q000494:pharmacology*; D051381:Rats
D009243:NAD; D009536:Niacinamide; D009249:NADP; D003577:Cytochrome P-450 Enzyme System