An immunoenzymatic method for improving the sensitivity of antigen measurement by electroimmunodiffusion techniques. Application to the quantification of human alpha-fetoprotein.
A double antibody technique of electroimmunodiffusion, which uses glucose oxidase-labelled sheep antibodies to rabbit immunoglobulins as second antibody, is described. Primary antigen-antibody reaction is carried out with a rabbit antiserum by electroimmundiffusion. The glucose oxidase-labelled immunoreagent, being of general application, can serve for the quantification of different antigens and is here used for measurement of low levels of human alpha-fetoprotein. Reproducible results in the range of 50-800 ng/ml were obtained with a variation coefficient of 5 to 10%.
D000818:Animals; D000906:Antibodies; D000941:Antigens / Q000032:analysis*; D006528:Carcinoma, Hepatocellular / Q000097:blood; D005260:Female; D005312:Fetal Blood / Q000032:analysis; D005326:Fetal Proteins / Q000032:analysis*; D005949:Glucose Oxidase; D006801:Humans; D005779:Immunodiffusion / Q000379:methods*; D007553:Isotope Labeling; D008113:Liver Neoplasms / Q000097:blood; D011247:Pregnancy; D011817:Rabbits / Q000276:immunology; D012756:Sheep / Q000276:immunology; D000509:alpha-Fetoproteins / Q000032:analysis*
D000906:Antibodies; D000941:Antigens; D005326:Fetal Proteins; D000509:alpha-Fetoproteins; D005949:Glucose Oxidase