Incorporation of inositol into intact red blood cells. II. Enhancement of gas transport in inositol hexaphosphate-loaded red blood cells.
The gas-transport function of red blood cells which have incorporated inositol hexaphosphate is significantly improved by fusion with effector-loaded lipid vesicles. "Right-shifts" of the O2-binding curves of inositol hexaphosphate-loaded red blood cells with half-saturation pressures at 37 degrees C up to 98 mmHg are observed. The transformation of 56% of the intracellular haemoglobin into the low affinity state corresponds to an optimum increase of the O2-release capacity of 270%. The CO2 transport is also correspondingly enhanced. The treated red blood cells show no inositol hexaphosphate depletion during a reasonable time.
D002245:Carbon Dioxide / Q000097:blood*; D004905:Erythrocyte Aging; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010100:Oxygen / Q000097:blood*; D010313:Partial Pressure; D010833:Phytic Acid / Q000097:blood* / Q000494:pharmacology
D002245:Carbon Dioxide; D010833:Phytic Acid; D010100:Oxygen