title: Demonstration of membrane-bound immunoglobulin determinants and the significance of a quantitative characterization of the antisera used.
abstract: Lymphocytes from 22 healthy blood donors were tested for the presence of immunoglobulin determinants on their surfaces by indirect membrane immunofluorescence. The proportion of MemIF+ cells was, on the average, 8.7% (3-18) for IgM and 15.8% (8-28) for IgG. The small differences in the proportion of MemIF+ lymphocytes upon use of various anti-IgM sera could be attributed mainly to the different antibody concentrations. The importance of a quantitative characterization of the antisera used was pointed out.
mesh_terms: D000328:Adult; D000888:Antibodies, Anti-Idiotypic; D000939:Epitopes; D005455:Fluorescent Antibody Technique / Q000379:methods; D006801:Humans; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008214:Lymphocytes / Q000276:immunology*; D008875:Middle Aged; D011947:Receptors, Antigen, B-Cell / Q000032:analysis*
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000939:Epitopes; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011947:Receptors, Antigen, B-Cell