The use of 3H serotonin release from mast cells of the mouse as an assay for mediator liberation.
The release of 3H 5-HT from murine mast cells is shown to be a simple reproducible method for studying the activation of such cells by various agents. 3H-serotonin was taken up by peritoneal cell suspensions in vitro and was released by antigen, Concanavalin A, Forssman antiserum, anti-mouse immunoglobulin, or a polypeptide antibiotic, Cinnamycin.
D000818:Animals; D003208:Concanavalin A / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005260:Female; D006636:Histamine Release; D007106:Immune Sera / Q000494:pharmacology; D008407:Mast Cells / Q000378:metabolism*; D051379:Mice; D008811:Mice, Inbred DBA; D010047:Ovalbumin / Q000276:immunology; D012701:Serotonin / Q000378:metabolism*; D013302:Streptomyces / Q000276:immunology; D014316:Tritium
D007106:Immune Sera; D014316:Tritium; D003208:Concanavalin A; D012701:Serotonin; D010047:Ovalbumin