[Changes in the chromatin structure of hepatocyte nuclei of rats trained to hypoxia].
Structure of chromatin in the nuclei of the isolated surviving hepatocytes and in the isolated nuclei of hepatocytes were studied by fluorochroming with acridine orange and by microfluorimetry of fluorescenc connected with the stain chromatin at 530 and 590 nm in intact rats and in the animals trained to hypoxia in a pressure chamber for 60 days. The nuclei of hepatocytes of intact rats were distributed by fluorescence at 530 nm into three classes with the intensity ratio of 1:2:4; as to the nuclei of hepatocytes of the rats trained to hypoxia - they formed a single class corresponding to the second class of control. In intact rats the ratio of the fluorescence intensity at 590 nm to such at 530 nm (alpha coefficient) formed normal distribution; in trained rats - a bimodal distribution with a shift of the maximum in the direction of reduction and increase of alpha in comparison with control. It is supposed that in hypoxia there is a repression of one and depression of other genes in the chromatine of the nuclei of the liver.
D000166:Acridines; D000222:Adaptation, Physiological; D000818:Animals; D002843:Chromatin / Q000378:metabolism*; D006651:Histocytochemistry; D000860:Hypoxia / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D051381:Rats; D013194:Staining and Labeling
D000166:Acridines; D002843:Chromatin
Izmeneniia struktury khromatina v iadrakh gepatotsitov krys, trenirovannykh k gipoksii