Characteristics of azurin from Pseudomonas aeruginosa via 270-MHz 1H nuclear magnetic resonance spectroscopy.
Assignments of resonances in the 1H nmr spectra of Cu(I) azurin to proton groups in the protein are discussed in detail. Comparisons are drawn between Cu(I), Cu(II), apo, Hg(II), and Co(II) azurin samples. Redox titration of Cu(I) azurin with K3Fe(CN)6, is used to correlate Cu(I) and Cu(II) 1H nmr spectral features, and observed line broadenings deriving from Cu(II) paramagnetic effects are used to deduce the distances of assigned proton groups from the copper center. Histidine residues are characterized in terms of pK values, rates of acid-base exchange near the the pK, and rates of C2H exchange with solvent deuterium. The possibility of histidine involvement in the azurincytochrome 551 electron exchange mechanism is discussed. A small number of NH protons observed to be distinctively inert to 2H exchange with solvent 2H2O, in the Cu(I) protein, are found to show increased lability on removal of the metal.
D001400:Azurin; D001426:Bacterial Proteins; D001665:Binding Sites; D003300:Copper; D005292:Ferricyanides; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D010084:Oxidation-Reduction; D011485:Protein Binding; D011487:Protein Conformation; D011550:Pseudomonas aeruginosa / Q000032:analysis*
D001426:Bacterial Proteins; D005292:Ferricyanides; D001400:Azurin; D003300:Copper