title: Proliferation of non-neuronal cells in spinal cords of irradiated, immature rats following transection of the sciatic nerve.
abstract: Transection of a peripheral nerve not only elicits changes in the injured neurons but also results in an increase in non-neuronal cells, considered by most workers to be neuroglia, in the region of these neurons. Since studies in this laboratory have shown that the neuroglial population of spinal cords of immature rats can be reduced markedly by ionizing radiation, the present investigation was undertaken to determine if this reaction would occur in the irradiated spinal cord following transection of the sciatic nerve. In order to answer this question the sciatic nerve was sectioned unilaterally at 17 days of age (14 days post-irradiation). Sham-irradiated littermates served as controls. Light microscopic examination showed an increase in non-neuronal cells throughout the gray matter on the side of axotomy in spite of a decreased neuroglial population in the 2,000 R and 3,000 R groups. These cells were scattered in the neuropil or were adjacent to injured neuronal perikarya in the anterior horn. Qualitatively similar reactions occurred in the 500 R and 1,000 R groups and in shamirradiated controls. Whether the magnitude of response is the same in all groups is currently under investigation, as are questions dealing with the origins of the reactive cells.
mesh_terms: D000367:Age Factors; D000818:Animals; D009457:Neuroglia / Q000166:cytology*; D010477:Perfusion; D011829:Radiation Dosage; D011830:Radiation Effects; D051381:Rats; D012584:Sciatic Nerve / Q000601:surgery*; D013116:Spinal Cord / Q000166:cytology / Q000528:radiation effects*; D013194:Staining and Labeling; D013376:Substantia Gelatinosa / Q000166:cytology