Preparation and some properties of human serum kallikrein immobilized on ARM-Sepharose.
A highly purified human serum kallikrein immobilized on CH-Sepharose 4-B was obtained. KM values for N-benzoyl-L-arginine ethyl ester and N-tosyl-L-arginine methyl ester hydrolysis of this preparation were 1.10 x 10(-3) M and 3.6 x 10(-4) M, respectively; pH optimum of hydrolysis of these esters were found to be 8.2 and 8.5, respectively. The immobilized kallikrein possessed kininogenase activity and was capable of activating prekallikrein.
D001120:Arginine / Q000031:analogs & derivatives / Q000378:metabolism; D004800:Enzymes, Immobilized; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007610:Kallikreins / Q000302:isolation & purification / Q000378:metabolism*; D014106:Tosylarginine Methyl Ester / Q000378:metabolism
D004800:Enzymes, Immobilized; D014106:Tosylarginine Methyl Ester; D001120:Arginine; D007610:Kallikreins