Phosphatidylseryl-transfer RNA in Bacillus licheniformis 749/C.
Phosphatidylserine has been found in extracts of Bacillus licheniformis made under alkaline conditions but not under neutral or acidic ones and was derived from the tRNA fraction. In tRNA preparations kept below neutrality during purification, phosphatidylserine was the only phospholipid released when the pH was raised to 9.0. The amount of bound phosphatidylserine could be increased by incubating tRNA from B. licheniformis or Escherichia coli with CTP and phosphatidic acid in the presence of an S-30 extract from either organism. The tRNA carrying phosphatidylserine has been separated from the bulk of the tRNA by DEAE-Sephadex chromatography in the presence of a detergent. On deaminoacylation of this material and rechromatography on DEAE-Sephadex, a number of peaks were found, indicating that this behavior is not confined to a single isoaccepting species.
D001407:Bacillus / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D010405:Penicillinase / Q000096:biosynthesis*; D010718:Phosphatidylserines / Q000378:metabolism*; D012329:RNA, Bacterial / Q000378:metabolism; D012343:RNA, Transfer / Q000032:analysis / Q000378:metabolism*; D001618:beta-Lactamases / Q000096:biosynthesis*
D010718:Phosphatidylserines; D012329:RNA, Bacterial; D012343:RNA, Transfer; D010405:Penicillinase; D001618:beta-Lactamases