Effect of preparatory techniques on the gross morphology of Mycoplasma hominis.
A method is presented whereby cells of Mycoplasma hominis can be prepared with minimal distortion for electron microscopy. After the addition of glutaraldehyde to broth cultures, incubation is continued for 1 h. The cells are then collected by centrifugation, washed in distilled water, and used for negative-contrast preparations.
D001431:Bacteriological Techniques; D003470:Culture Media; D005976:Glutaral; D008854:Microscopy, Electron; D009174:Mycoplasma / Q000648:ultrastructure*; D010772:Phosphotungstic Acid; D013194:Staining and Labeling
D003470:Culture Media; D010772:Phosphotungstic Acid; D005976:Glutaral