Immunochemical studies on bacterial blood group substances. VIII. The structure of oligosaccharides from blood group H-active polysaccharide of Escherichia coli 2B-V.
Blood group H-active  polysaccharide has been prepared from "smooth" strain Escherichia coli 2B-V by Freeman's method, alpha-Fucosidase derived from Bacillus fluminans caused the liberation of fucose from this polysaccharide, together with concomitant loss of blood group H activity. The results of quantitative microanalysis, borohydride reduction, the Morgan-Elson reaction and enzymic hydrolysis with betagalactosidase using isolated oligosaccharides obtained by partial acid hydrolysis indicated that the O-specific side chain of the polysaccharide has a pentassaccharide unit which is beta-D-Gal-(1 leads to 3)-D-GalNAc-(1 leads to 3)-D-GalNAc-Fuc with a D-glucose residue bound at some undetermined point on this structure. It was considered that terminal non-reducing fucose of the polysaccharide was liberated by partial acid hydrolysis.
D000116:Acetylgalactosamine / Q000032:analysis; D000117:Acetylglucosamine / Q000032:analysis; D001789:Blood Group Antigens; D000939:Epitopes; D004926:Escherichia coli / Q000032:analysis*; D005643:Fucose / Q000032:analysis; D005688:Galactosamine / Q000032:analysis; D005690:Galactose / Q000032:analysis; D005944:Glucosamine / Q000032:analysis; D005947:Glucose / Q000032:analysis; D009844:Oligosaccharides / Q000032:analysis*; D011135:Polysaccharides, Bacterial / Q000032:analysis*; D013237:Stereoisomerism
D001789:Blood Group Antigens; D000939:Epitopes; D009844:Oligosaccharides; D011135:Polysaccharides, Bacterial; D005643:Fucose; D005688:Galactosamine; D005947:Glucose; D000116:Acetylgalactosamine; D005944:Glucosamine; D000117:Acetylglucosamine; D005690:Galactose