The effect of temperature and ouabain on the breakdown of ATP and 2,3-bisphosphoglycerate in glucose-depleted human red cells.
Ouabain exerts only a minor effect on ATP breakdown at 37 degrees C and practically none at 4 degrees C. The effect is less at higher pH values. At 4 degrees C the rates of breakdown at ATP, AMP and of 2,3 P2G are nearly two orders of magnitude less than at 37 degrees C.
D000244:Adenosine Diphosphate / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000097:blood*; D001786:Blood Glucose / Q000378:metabolism*; D002478:Cells, Cultured; D004163:Diphosphoglyceric Acids / Q000097:blood*; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010042:Ouabain / Q000494:pharmacology*; D013696:Temperature
D001786:Blood Glucose; D004163:Diphosphoglyceric Acids; D000249:Adenosine Monophosphate; D010042:Ouabain; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate