Quantitative studies of Gm allotypes. I. Reappraisal of the method using an autoanalyzer.
In this study, various parameters for the autoanalyzer hemagglutination-inhibition circuit applied to Gm studies, were examined. Details are given of modifications of this circiut and related methodology, that improve its precision and the reproducibility of results: use of a cells counter, effects of temperature, PVP, Tween 20, anti-Gm concentration, etc. The effects of age, storage and freezing on quantitative Gm allotypes antigenicity are also stressed.
D000888:Antibodies, Anti-Idiotypic / Q000032:analysis; D001322:Autoanalysis / Q000379:methods; D001793:Blood Preservation; D000939:Epitopes; D005069:Evaluation Studies as Topic; D005260:Female; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D007126:Immunoglobulin Allotypes; D008297:Male; D011205:Povidone / Q000494:pharmacology; D013223:Statistics as Topic; D013696:Temperature
D000888:Antibodies, Anti-Idiotypic; D000939:Epitopes; D007126:Immunoglobulin Allotypes; D011205:Povidone