Removal of blood group determinants from bovine erythrocyte membranes. 2. Degradation of ghosts by butanol and pyridine.
Ghosts produced from erythrocytes collected from six different cattle were degraded with butanol and pyridine. Of a total of 38 different antigenic determinants available for investigation among the six cows, F, V, J and L were the only specificities detected in the subfractions resulting from either method of degradation. After butanol degradation V, J and L antigens were found in the soluble protein fraction, while F was found in the insoluble protein. Pyridine digestion resulted in all four determinants being detected in the sialoprotein layer, while J was found in the lipoprotein as well. All antigens were relatively weak, being detected in inhibition strengths of 10.0 to 1.25 mg/ml.
D000818:Animals; D000440:Butanols / Q000494:pharmacology*; D002417:Cattle / Q000097:blood*; D002458:Cell Fractionation; D002462:Cell Membrane / Q000276:immunology; D000939:Epitopes; D004912:Erythrocytes / Q000276:immunology*; D006385:Hemagglutination Inhibition Tests; D011725:Pyridines / Q000494:pharmacology*
D000440:Butanols; D000939:Epitopes; D011725:Pyridines