Pyridoxal phosphatase: cytochemical localization in GERL and other organelles of rat neurons.
A phosphatase, hydrolyzing pyridoxal-5-phosphate (P5P), a physiologically active component of the vitamin B6 complex and an essential co-enzyme in the synthesis of neurotransmitters, has been localized cytochemically in the perikarya of neurons in the peripheral, autonomic and central nervous systems of the rat. Neurons in dorsal root ganglia, sympathetic ganglia and ventral horn of spinal cord were studied by light and electron microscopy, while Purkinje cells, neurons in the dentate nucleus of the cerebellum, thalamus, and hypothalamus were studied by light microscopy only. Optimal conditions for demonstrating this activity in aldehyde-fixed tissue were determined with dorsal root ganglia. At the optimal pH of 5.0, neurons in these ganglia and in all other neurons studied show pyridoxal-5-phosphatase (P5Pase) activity in GERL. Small neurons in dorsal root ganglia also display enzyme activity in the endoplasmic reticulum (ER); activities in GERL and ER are also appreciably high at neutral pH. Small and large neurons in these ganglia, and neurons of sympathetic ganglia, show variable P5Pase activity in the Golgi apparatus. These localizations differ from the usual sites of both acid phosphatase and alkaline phosphatase activities. The P5Pase activity, demonstrated cytochemically, is a new acid hydrolase activity in GERL.
D000818:Animals; D004721:Endoplasmic Reticulum / Q000201:enzymology*; D005404:Fixatives; D005725:Ganglia, Autonomic / Q000201:enzymology; D005727:Ganglia, Spinal / Q000201:enzymology; D006863:Hydrogen-Ion Concentration; D007031:Hypothalamus / Q000201:enzymology; D008297:Male; D009046:Motor Neurons / Q000201:enzymology; D009474:Neurons / Q000201:enzymology* / Q000648:ultrastructure; D009940:Organoids / Q000201:enzymology; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis*; D011732:Pyridoxal Phosphate; D051381:Rats; D013788:Thalamus / Q000201:enzymology
D005404:Fixatives; D011732:Pyridoxal Phosphate; C022493:pyridoxal-5-phosphatase; D010744:Phosphoric Monoester Hydrolases