Measurement of dengue virus-specific double-stranded ribonucleic acid in infected primate cell extracts by microquantitative complement-fixation methods.
Microquantitative complement-fixation methods have been applied to the detection and quantitation of double-stranded RNA (dsRNA) in cytoplasmic extracts of uninfected and dengue virus-infected primate cell lines. LLC-MK2 and PGLC-33H cells exhibited increased levels of dsRNA following dengue virus infection. Cytoplasmic extracts of infected and uninfected LLC-MK2 cells subjected to density gradient analysis showed that increased levels of dsRNA were associated with structures believed to be virus-specific. Primate cell dsRNA and dengue dsRNA preferentially reacted with rabbit anti-rIn-rCn, while reovirus dsRNA and rAn-containing synthetic duplexes preferentially reacted with anti-rAn-rUn.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D002460:Cell Line; D002474:Cell-Free System; D002499:Centrifugation, Density Gradient; D002521:Cercopithecus; D003168:Complement Fixation Tests; D003716:Dengue Virus / Q000276:immunology*; D000939:Epitopes; D000882:Haplorhini; D006801:Humans; D008253:Macaca mulatta; D012367:RNA, Viral / Q000032:analysis*
D000914:Antibodies, Viral; D000939:Epitopes; D012367:RNA, Viral