Glycosaminoglycan staining in diseasesed dog skin.
Diseased skin of dogs was stained using the critical electrolyte concentration-Alcian Blue method, PAS methods, and the high iron diamine technique. Digestion with testicular hyaluronidase and chondroitinase was also used to evaluate the staining results. Diseased skin exhibits a tendency for the glycosaminoglycans to revert to the condition seen in juvenile normal skin: epidermal glycoprotein content falls, total glycosaminoglycan content and the proportion undigested by hyaluronidase rises, and sulphation falls. In collagen, both hyaluronidase-stable material and sulphation increase, but follicle basement membrane does not show this trend towards the juvenile state.
D000818:Animals; D004283:Dog Diseases / Q000378:metabolism / Q000473:pathology*; D004285:Dogs; D006025:Glycosaminoglycans / Q000378:metabolism*; D006651:Histocytochemistry; D012867:Skin / Q000378:metabolism / Q000473:pathology*; D012871:Skin Diseases / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D013045:Species Specificity; D013194:Staining and Labeling
D006025:Glycosaminoglycans