Blastogenic response and EBNA induction in human lymphocytes by Epstein-Barr virus only requires B cells but not macrophages.
The postulated role of macrophages in the primary infection of human lymphocytes by Epstein-Barr virus (EBV) was examined. Macrophage removal had no effect on the blastogenic response but slightly reduced the percentage of EBNA-positive cells induced by the B 95-8 virus strain. Both the rate of DNA synthesis and the appearance of EBNA-positive cells could rather be related to the number of B cells in the infected populations. Macrophages are thus not required for the initiation of EBV-induced lymphocyte transformation.
D000956:Antigens, Viral / Q000096:biosynthesis*; D001402:B-Lymphocytes / Q000276:immunology / Q000502:physiology*; D004247:DNA / Q000096:biosynthesis; D004268:DNA-Binding Proteins / Q000096:biosynthesis*; D019309:Epstein-Barr Virus Nuclear Antigens; D006566:Herpesviridae Infections / Q000097:blood / Q000378:metabolism / Q000382:microbiology; D004854:Herpesvirus 4, Human / Q000276:immunology / Q000502:physiology*; D006801:Humans; D008213:Lymphocyte Activation / Q000276:immunology / Q000502:physiology*; D008214:Lymphocytes / Q000378:metabolism* / Q000382:microbiology*; D008264:Macrophages / Q000276:immunology / Q000502:physiology*
D000956:Antigens, Viral; D004268:DNA-Binding Proteins; D019309:Epstein-Barr Virus Nuclear Antigens; D004247:DNA