[Zincporphyrin pigments in the botryoid tissue of Haemopis sanguisuga L. and their localization by diaminobenzidine-H2O2 reaction (author's transl)].
From isolated botrydiosomes of Haemopis sanguisuga L. a pigment was extracted, separated by preparative paper chromatography and analysed chemically. Chromatographically the pigment was found to be composed of two fractions, which has been identified as zincporphyrin compounds, which are able to oxidize of diaminobenzidine in the presence of H2O2. As proved by ultrahistochemical method, the product of hte diaminobenzidine-H2O2 reaction is associated in the botryoid cells with the tubules foth of the endoplasmic reticulum and of botrydiosomes. The proboble functions of the zincporphyrins in the oxidizing processes are discussed.
D015100:3,3'-Diaminobenzidine; D000818:Animals; D004721:Endoplasmic Reticulum / Q000032:analysis; D006861:Hydrogen Peroxide; D007865:Leeches / Q000032:analysis* / Q000648:ultrastructure; D009940:Organoids / Q000032:analysis; D010084:Oxidation-Reduction; D011166:Porphyrins / Q000032:analysis*; D013194:Staining and Labeling; D015032:Zinc / Q000032:analysis*
D011166:Porphyrins; D015100:3,3'-Diaminobenzidine; D006861:Hydrogen Peroxide; D015032:Zinc
Zinkporphyrin-Pigmente im Botryoidgewebe von Haemopis sanguisuga L. und ihre Lokalisation mit der Diaminobenzidin-H22-O Reaktion