Beta-glucuronidase activity of Yoshida ascites hepatomas of different drug-sensitivity and its change after treatment of host rats with various anticancer agents.
Change in beta-glucuronidase activity of six Yoshida ascites hepatomas was examined after treatment of host rats with one of 12 anticancer agents. The hepatomas, AH-66F, AH-130, AH-109A, AH-60C, and AH-44, in decreasing order showed more or less distinct increase in beta-glucuronidase activity after treatment of the rats with Nitromin, Endoxan, 864-T, Carbazilquinone, Mitomycin-C, Toyomycin, Daunomycin, Neocarzinostatin, vincristine sulfate, 5-fluorouracil, or cytosine arabinoside only when the cytological effect was positive. Moreover, degree of the increase was generally correlated with that of cytological effect. Bleomycin was ineffective either enzymically or cytologically. AH-66 was insensitive to any of the agents tested in increasing beta-glucuronidase activity and showed only a very slight cytological response to some of the agents. Acid deoxyribonuclease behaved like beta-glucuronidase but to a lesser extent. The above order of drug sensitivity of the hepatomas was not in parallel with that of normal beta-glucuronidase level, which also did not correlate with the life span of host rats.
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001761:Bleomycin / Q000494:pharmacology; D002225:Carbazilquinone / Q000494:pharmacology; D006528:Carcinoma, Hepatocellular / Q000201:enzymology*; D002865:Chromomycins / Q000494:pharmacology; D003520:Cyclophosphamide / Q000494:pharmacology; D003561:Cytarabine / Q000494:pharmacology; D003630:Daunorubicin / Q000494:pharmacology; D003851:Deoxyribonucleases / Q000378:metabolism; D005472:Fluorouracil / Q000494:pharmacology; D005966:Glucuronidase / Q000378:metabolism*; D008113:Liver Neoplasms / Q000201:enzymology*; D008297:Male; D008698:Mesylates / Q000494:pharmacology*; D008937:Mitomycins / Q000494:pharmacology; D009374:Neoplasms, Experimental; D009574:Nitro Compounds / Q000494:pharmacology; D051381:Rats; D012517:Sarcoma, Yoshida / Q000201:enzymology; D014105:Tosyl Compounds / Q000494:pharmacology; D014750:Vincristine / Q000494:pharmacology; D009353:Zinostatin / Q000494:pharmacology
D000970:Antineoplastic Agents; D002865:Chromomycins; D008698:Mesylates; D008937:Mitomycins; D009574:Nitro Compounds; D014105:Tosyl Compounds; D003561:Cytarabine; D001761:Bleomycin; D002225:Carbazilquinone; D014750:Vincristine; D003520:Cyclophosphamide; D009353:Zinostatin; D003851:Deoxyribonucleases; D005966:Glucuronidase; D005472:Fluorouracil; D003630:Daunorubicin