[Dependence of heterochromatin differential staining on the time of its reduplication and the degree of condensation].
A comparison of the chromosomes banding pattern after G-and C-staining with the time of DNA reduplication and the degree of chromosome condensation, was carried out using Chinese hamster metaphase chromosomes. Chromosome condensation was studied under 5-bromodeoxyuridine and 5-bromodeoxycytidine treatment. All the chromosomal segments stained with C-technique are also stainable with G-technique, while only some G-positive segments are capable to be C-bands. C-bands are heterochromatic segments characterized by extremely late replication and great delay in condensation under the analog action, while G-bands are segments with earlier labelling and irregular decondensation. The data obtained suggest a close correlation between the capability of chromosomal region of G- and C- staining and the degree of its heterochromatinization.
D000782:Aneuploidy; D000818:Animals; D001345:Autoradiography; D001972:Bromodeoxycytidine / Q000494:pharmacology; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002460:Cell Line; D002478:Cells, Cultured; D002875:Chromosomes / Q000032:analysis; D006224:Cricetinae; D003584:Cytological Techniques; D004261:DNA Replication; D006570:Heterochromatin / Q000032:analysis*; D007621:Karyotyping; D008938:Mitosis / Q000187:drug effects; D013194:Staining and Labeling; D013997:Time Factors; D014316:Tritium
D006570:Heterochromatin; D014316:Tritium; D001972:Bromodeoxycytidine; D001973:Bromodeoxyuridine
Zavisimost' differentsial'noi okrashivaemosti geterokhromatina ot vremeni ego reduplikatsii i stepeni kondensatsii