Spectrofluorometric assay of gentamicin in serum.
A spectrofluorometric method has been developed for the assay of gentamicin in serum. The fluorogen of this reaction is the Zn2+ chelate of N-pyridoxylidene gentamicin. The ratio of gentamicin to Zin is 1.0. Interfering compounds in serum are removed by chromatography on Bio-Rex 70. Recovery of added gentamicin is between 90 and 100%. Determination of gentamicin levels in patient sera correlate well with those done by bioassay. This assay is relatively fast (about 1 h), and requires 1.0 ml of serum. It also can be applied to the determination of other deoxystreptamine antibiotics such as kanamycin and tobramycin.
D001681:Biological Assay; D005839:Gentamicins / Q000097:blood*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D012995:Solubility; D013050:Spectrometry, Fluorescence; D015032:Zinc
D005839:Gentamicins; D015032:Zinc