title: Chloride-dependent restoration of coupling by oligomycin in rat liver mitochondria.
abstract: In rat liver mitochondria suspended in KC1 medium, oligomycin interfered with the effect of uncouplers on energy conservation. It antagonized the effect of uncouplers that are weak acids (2,4-dinitrophenol etc.), but enhanced that of the lipid-penetrating cation NN-dimethyl-N'N'-dibenzylammonium. Oligomycin caused none of the above effects when Br- or NO-/3 was substituted for C1- as the major anionic species in the assay medium. The concentration of oligomycin that exerted the above-mentioned effects was lower than that necessary for the inhibition of energy transfer, but was in the range that induced C1- permeation through the cristae membrane. The possible connexion between the effect of oligomycin on C1- permeation and its interference with the action of uncouplers is discussed.
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology; D000251:Adenosine Triphosphatases / Q000032:analysis; D000818:Animals; D001965:Bromides / Q000378:metabolism; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone / Q000037:antagonists & inhibitors; D002712:Chlorides / Q000378:metabolism*; D001728:Dicumarol / Q000037:antagonists & inhibitors; D004140:Dinitrophenols / Q000037:antagonists & inhibitors / Q000494:pharmacology; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D008933:Mitochondrial Swelling / Q000187:drug effects; D009566:Nitrates / Q000378:metabolism; D009840:Oligomycins / Q000494:pharmacology*; D010085:Oxidative Phosphorylation / Q000187:drug effects; D010101:Oxygen Consumption / Q000187:drug effects; D000644:Quaternary Ammonium Compounds / Q000037:antagonists & inhibitors; D051381:Rats; D014475:Uncoupling Agents / Q000037:antagonists & inhibitors*
chemical_list: D001965:Bromides; D002712:Chlorides; D004140:Dinitrophenols; D009566:Nitrates; D009840:Oligomycins; D000644:Quaternary Ammonium Compounds; D014475:Uncoupling Agents; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone; D000244:Adenosine Diphosphate; D001728:Dicumarol; D000251:Adenosine Triphosphatases