Restoration of specific immunity against SV40 tumor-specific transplantation antigen to lymphoid cells from tumor-bearing mice.
Specific cell-mediated immunity to SV40 tumor-specific transplantation antigen (TSTA) in BALB/c mice undergoing progressive tumorigenesis by syngeneic SV40-transformed cells (VLM) was investigated in vivo using a tumor-cell neutralization test. Specific cellular reactivity to SV40 TSTA was not detected in BALB/c mice bearing large tumors (10-15 mm mean diameter) but was demonstrable after tumor excision. Specific cytotoxic reactivity against syngeneic SV40-transformed cells in vivo could be restored to lymphoid cells from VLM tumor-bearing mice either by culturing the lymphoid cells in vitro or by treating them with papain or trypsin. Enzyme-treated lymphoid cells from MCA tumor-bearing BALB/c mice had no cytotoxic reactivity against VLM cells. These studies suggest that tumor-bearing hosts possess lymphocytes which are sensitized to the TSTA of the tumor but that the reactivity of these lymphocytes is blocked.
D000818:Animals; D000951:Antigens, Neoplasm; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000187:drug effects; D000939:Epitopes; D006649:Histocompatibility Antigens; D007111:Immunity, Cellular; D007114:Immunization; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000276:immunology*; D010447:Peptide Hydrolases / Q000494:pharmacology; D013539:Simian virus 40 / Q000276:immunology*; D013268:Stimulation, Chemical; D014185:Transplantation, Isogeneic
D000951:Antigens, Neoplasm; D000939:Epitopes; D006649:Histocompatibility Antigens; D010447:Peptide Hydrolases