The use of indirect immunofluorescence to evaluate the gel filtration method of fractionating human immunoglobulins.
The indirect immunofluorescent technique has been shown to be a more sensitive method than either immunoelectrophoresis or gel filtration for testing the efficacy of the gel filtration method in fractionating human immunoglobulins. It has been confirmed that IgM fractions of some sera from patients with multiple sclerosis, contain antibody which reacts with measles virus-infected tissue culture cells.
D000042:Absorption; D002478:Cells, Cultured; D005591:Chemical Fractionation; D002850:Chromatography, Gel / Q000592:standards*; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007136:Immunoglobulins; D008457:Measles / Q000276:immunology; D008459:Measles virus / Q000276:immunology; D009103:Multiple Sclerosis / Q000276:immunology; D012217:Rheumatoid Factor / Q000302:isolation & purification; D005719:gamma-Globulins
D007106:Immune Sera; D007136:Immunoglobulins; D005719:gamma-Globulins; D012217:Rheumatoid Factor