Inhibition of glutamine synthetase activity by manganous ions in a cytosol extract of rat liver.
Glutamine synthetase activity in a cytosol extract of liver was inhibited non-competitively by Mn2+ ions. The apparent Ki for Mn2" in the presence of phosphate was 8 micro M. Inhibition of glutamine synthetase by intracellular Mn2+ may contribute to the very low rates of glutamine synthesis observed in perfused liver and isolated hepatocytes.
D000818:Animals; D003600:Cytosol / Q000187:drug effects / Q000201:enzymology; D005260:Female; D005974:Glutamate-Ammonia Ligase / Q000037:antagonists & inhibitors*; D066298:In Vitro Techniques; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008345:Manganese / Q000494:pharmacology*; D051381:Rats
D008345:Manganese; D005974:Glutamate-Ammonia Ligase