Fibrin membrane endowed with biological function. IV. Formation of cross-links between fibrinogen (or fibrin) and ribonuclease by transglutaminase.
Transglutaminase from guinea pig liver catalyzed the formation of cross-links between fibrinogen (or fibrin) and ribonuclease. Using transglutaminase, immoblized ribonuclease was prepared by two separate methods: (1) fibrinogen-ribonuclease conjugates formed by transglutaminase were treated with thrombin to make fibrin membrane bound covalently to the enzyme; (2) fibrin polymer formed from fibrinogen with thrombin was covalently bound to ribonuclease by transglutaminase to make fibrin-ribonuclease conjugates.
D000818:Animals; D003432:Cross-Linking Reagents; D005337:Fibrin / Q000378:metabolism*; D005340:Fibrinogen / Q000378:metabolism*; D006168:Guinea Pigs; D006801:Humans; D008567:Membranes, Artificial; D012260:Ribonucleases / Q000378:metabolism*; D005723:gamma-Glutamyltransferase / Q000378:metabolism*
D003432:Cross-Linking Reagents; D008567:Membranes, Artificial; D005337:Fibrin; D005340:Fibrinogen; D005723:gamma-Glutamyltransferase; D012260:Ribonucleases