Evaluation of platelet cryopreservation techniques by isolated kidney perfusion.
The isolated perfused rabbit kidney, a model system used previously to assess platelet function, was adapted for evaluation of human platelet cryopreservation techniques. A new, simple, efficient device for controlling cooling rates before, during, and after freezing was used. Platelet concentrates frozen with 5 per cent dimethyl sulfoxide (DMSO) under different conditions were the most effective of those tried in maintaining the hemostatic function and vascular integrity of perfused kidneys. Our studies indicate that the isolated, perfused rabbit kidney can be used to evaluate platelet cryopreservation techniques and is potentially adaptable for studies of organ cryopreservation.
D000818:Animals; D001772:Blood Cell Count; D001792:Blood Platelets / Q000502:physiology; D001793:Blood Preservation; D002470:Cell Survival; D003018:Clot Retraction; D003911:Dextrans; D004121:Dimethyl Sulfoxide; D005615:Freezing; D006400:Hematocrit; D006895:Hydroxyethyl Starch Derivatives; D007668:Kidney / Q000473:pathology / Q000502:physiology; D009929:Organ Size; D010477:Perfusion; D011092:Polyethylene Glycols; D011312:Pressure; D011817:Rabbits; D013696:Temperature; D014556:Urine / Q000502:physiology
D003911:Dextrans; D006895:Hydroxyethyl Starch Derivatives; D011092:Polyethylene Glycols; D004121:Dimethyl Sulfoxide