Enzymoimmunoassay of human alpha-fetoprotein.
A non-competitive method for the determination of human alpha-fetoprotein (AFP) in serum, using a pure specific antibody linked to glucose oxidase is described. When applied to human AFP, this method gives reproducible results in the range 0.7 to 15 ng/ml, in a relatively short time (6 hr). AFP sera levels of healthy human adults, pregnant women and adults with liver diseases, were tested both by enzymoimmunoassay and radioimmunoassay. In all cases, good agreement was noted between the two methods.
D000327:Adsorption; D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D002845:Chromatography; D003911:Dextrans; D005326:Fetal Proteins / Q000032:analysis*; D005947:Glucose / Q000378:metabolism; D005949:Glucose Oxidase; D006801:Humans; D007106:Immune Sera; D007118:Immunoassay / Q000379:methods*; D007202:Indicators and Reagents; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay; D012995:Solubility; D000509:alpha-Fetoproteins / Q000032:analysis*
D000906:Antibodies; D003911:Dextrans; D005326:Fetal Proteins; D007106:Immune Sera; D007202:Indicators and Reagents; D000509:alpha-Fetoproteins; D005949:Glucose Oxidase; D005947:Glucose