[The evolution of the thickness of rabbit corneas in hydratation, the influence of pH and composition of the solution (author's transl)].
The swelling of excised, de epitheliated, rabbit corneas has been measured every five minutes during their immersion in a hydrating medium. The following media have been used: distilled H2O, Krebs-bicarbonate Ringer solution, HCl 0,01 N and NaCl 1 M/l. The swelling is very moderate with Krebs and NaCl, much higher and practically proportional to the duration of immersion with HCl, still faster with H2O. On the other hand, the influence of pH has been studied using 13 different solutions buffered from pH 1 to pH 13. A pronounced minimum of the swelling was found for pH 4, along with 3 relative maxima: the first around pH 2, the second around pH 8 and the third around pH 12. The interpretation of those results on a biochemical basis is discussed. For all these media, the solvent was distilled H2O. At last, two statistical distributions, based on 131 corneas, have been determined: one concerning the initial thickness and the other concerning the difference between the initial thickness of the two corneas of the same rabbit.
D000818:Animals; D003315:Cornea / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D011817:Rabbits; D012996:Solutions
D012996:Solutions
Evolution de l'épaisseur des cornées de lapins en cours d'hydratation. Influence du pH et de la composition du milieu hydratant.