C3, GBG, orosomucoid and haptoglobin polymorphisms. Improved staining methods.
C3, GBG, and orosomucoid polymorphisms were electrophoresed in a high-voltage agarose method which permitted the typing of 15-20 samples. The increased sensitivity of the dye Coommassie blue was used to stain the protein, and yielded higher resolution than amido black. The typing of haptoglobin samples, was facilitated by devising a method which utilizes the peroxidase activity of the haptoglobin-hemoglobin complex with 90-tolidine and hydrogen peroxide as substrates and 4-chloro-1-naphthol as coupler.
D003165:Complement System Proteins / Q000302:isolation & purification*; D004586:Electrophoresis; D006023:Glycoproteins / Q000302:isolation & purification*; D006242:Haptoglobins / Q000032:analysis; D006454:Hemoglobins; D006801:Humans; D008722:Methods; D009961:Orosomucoid / Q000302:isolation & purification*; D010544:Peroxidases / Q000032:analysis; D010641:Phenotype; D013194:Staining and Labeling
D006023:Glycoproteins; D006242:Haptoglobins; D006454:Hemoglobins; D009961:Orosomucoid; D003165:Complement System Proteins; D010544:Peroxidases