Aminoglycoside 3'-phosphotransferase III, a new phosphotransferase. Resistance mechanism.
The aminoglycoside phosphotransferase of Pseudomonas aeruginosa 21-75 was purified by affinity chromatography using dibekacin-Sephadex 4B or lividomycin A-Sepharose 4B followed by DEAE Sephadex A-50 chromatography. It had activities of both the known aminoglycoside 3'-phosphotransferases I and II, and transferred phosphate from ATP to the 3'-hydroxyl group of kanamycin A, ribostamycin and butirosin A and 5-hydroxyl group of lividomycin A. This enzyme was designated aminoglycoside 3'-phosphotransferase III. It showed strong substrate inhibition by kanamycin A and ribostamycin when their concentration exceeded 6 muM. Purification and characterization of this enzyme are reported.
D000617:Aminoglycosides; D002076:Butirosin Sulfate / Q000494:pharmacology; D007612:Kanamycin / Q000494:pharmacology; D010303:Paromomycin / Q000031:analogs & derivatives / Q000494:pharmacology; D010770:Phosphotransferases / Q000037:antagonists & inhibitors / Q000302:isolation & purification; D011550:Pseudomonas aeruginosa / Q000201:enzymology; D012271:Ribostamycin / Q000494:pharmacology
D000617:Aminoglycosides; D012271:Ribostamycin; D002076:Butirosin Sulfate; D007612:Kanamycin; D010303:Paromomycin; D010770:Phosphotransferases