Unexpectedly low incorporation of isotopic acetate into lipids of Ehrlich ascites tumor cells cultured in lipid-poor medium.
The present communication reports data on the lipid biosynthesis of Ehrlich ascites tumor cells grown in culture media supplemented with modified sera. Whereas the metabolisms of [14C]pyruvate and [14C]mevalonate are identical in all media tested, the incorporation of [14C] acetate is higher in medium with dialyzed serum than in medium with delipidized serum; it is suppressed in the absence of all lipids in culture medium. Cellular integrity is not impaired in modified media. The results indicate that acetyl-CoA synthetase of Ehrlich ascites tumor cells is not regulated by exogenous lipids as is known to be the case in nonmalignant cells.
D000106:Acetate-CoA Ligase / Q000378:metabolism; D000085:Acetates / Q000378:metabolism*; D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D002478:Cells, Cultured; D003470:Culture Media; D008055:Lipids / Q000008:administration & dosage / Q000096:biosynthesis*; D008798:Mevalonic Acid / Q000378:metabolism; D051379:Mice; D011773:Pyruvates / Q000378:metabolism
D000085:Acetates; D003470:Culture Media; D008055:Lipids; D011773:Pyruvates; D000106:Acetate-CoA Ligase; D008798:Mevalonic Acid