Functional characterization of an adhesive component from the embryonic chick neural retina.
We have developed a quantitative assay for tissue-specific adhesive components which is based on the agglutination of glutaraldehyde-fixed cells. At least 2 components are required for fixed-cell agglutination: a cell-surface ligand which is obtained from tissue culture-conditioned medium, and a soluble 'agglutinin' which accumulates in conditioned medium from monolayer cultures. Our results suggest that the surface-binding ligand and the agglutinin interact directly, resulting in tissue-specific agglutination of cells. The agglutination reaction exhibits divalent cation, temperature, and pH dependence. Several models of cell adhesion are described; the simplest of these which can account for the data is a multicomponent model in which the 2 adhesive components have structural roles.
D000373:Agglutinins; D000818:Animals; D002448:Cell Adhesion; D002452:Cell Count; D002642:Chick Embryo; D046508:Culture Techniques; D006863:Hydrogen-Ion Concentration; D008024:Ligands; D012160:Retina / Q000166:cytology*; D013696:Temperature
D000373:Agglutinins; D008024:Ligands