Evidence for different forms of human chorionic gonadotropin receptor complex in pseudopregnant rat ovaries.
After in vivo application of [125 I]-iodo-hCG to pseudopregnant rats, radioactivity extracted from ovarian particulate fractions can be eluted from Sephadex G--200 columns in three various fractions (Vo, Kav = 0.15, Kav = 0.27) differing from hCG (Kav = 0.35). By lowering the pH 50% of the radioactivity is precipitated and the resuspended material shows a conversion to the smaller complexes. The supernatant material is dissociated to [125I]-iodo-hCG. In alkaline milieu [125I]-iodo-HCG is dissociated completely from the complexes.
D000818:Animals; D006063:Chorionic Gonadotropin / Q000378:metabolism*; D002850:Chromatography, Gel; D005260:Female; D006863:Hydrogen-Ion Concentration; D010053:Ovary / Q000378:metabolism*; D011555:Pseudopregnancy / Q000378:metabolism*; D051381:Rats; D011956:Receptors, Cell Surface / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism
D006063:Chorionic Gonadotropin; D011956:Receptors, Cell Surface