In vitro viral transformation of human prostatic carcinoma.
Cell cultures derived from fetal human and prostatic adenocarcinoma were inoculated with SV40 virus. Within 6 weeks in vitro morphologic transformation occurred, after which the cells proliferated rapidly. They exhibited the SV40 T-antigen by immunofluorescence and survived propagation beyond the limited lifespan of uninfected cultures. We suggest that this may be a useful technique in establishing stable cell lines of human prostate.
D000230:Adenocarcinoma / Q000201:enzymology / Q000473:pathology*; D000951:Antigens, Neoplasm / Q000032:analysis; D002455:Cell Division; D002460:Cell Line; D002470:Cell Survival; D002471:Cell Transformation, Neoplastic / Q000473:pathology; D006801:Humans; D008297:Male; D011467:Prostate / Q000196:embryology; D011470:Prostatic Hyperplasia / Q000473:pathology; D011471:Prostatic Neoplasms / Q000201:enzymology / Q000473:pathology*; D013539:Simian virus 40 / Q000276:immunology
D000951:Antigens, Neoplasm