The histochemical application of dansylhydrazine as a fluorescent labeling reagent for sialic acid in cellular glycoconjugates.
A new method for the fluorescent staining of stalic acid-containing glycoconjugates in fixed tissues is described. The procedure uses mild periodate oxidation, followed by condensation with dansylhydrazine and reduction of the hydrazones to hydrazines. The specificity of the reaction for sialic acid is tested on model glycoconjugates. The procedure gives superior resolution in comparison to the standard periodate Schiff procedure for cellular carbohydrates.
D000818:Animals; D003619:Dansyl Compounds; D005732:Gangliosides / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis; D006834:Hydrazines; D007422:Intestines / Q000166:cytology; D008856:Microscopy, Fluorescence; D010504:Periodic Acid; D051381:Rats; D012545:Schiff Bases; D012794:Sialic Acids / Q000032:analysis*; D013194:Staining and Labeling / Q000379:methods*
D003619:Dansyl Compounds; D005732:Gangliosides; D006023:Glycoproteins; D006834:Hydrazines; D012545:Schiff Bases; D012794:Sialic Acids; D010504:Periodic Acid