Studies on the antigenic determinants of the Thy-1.2 alloantigen as expressed by the murine lymphoblastoid line S-49.1 TB-2-3.
Sialic acid has been shown to be part of the antigenic determinant of the Thy-1.2 alloantigen as expressed by the murine cell line S-49.1 TB-2-3 (S-49). This conclusion is based on the loss of cytotoxic inhibitory activity by the action of neuraminidase on the Thy-1.2 alloantigen. Also, sialic acid has the ability to inhibit the cytotoxic assay of AKR anti-C3H Thy-1.2 serum for S-49 cells. Further evidence for the protein nature of the Thy-1.2 alloantigen is apparent by trypsin digestion. Possibly the Thy-1.2 alloantigen as expressed on S-49 cells is a glycoprotein.
D000818:Animals; D002460:Cell Line; D003601:Cytotoxicity Tests, Immunologic; D000939:Epitopes; D066298:In Vitro Techniques; D007519:Isoantigens / Q000032:analysis*; D051379:Mice; D012794:Sialic Acids / Q000032:analysis; D013950:Thymus Gland / Q000276:immunology*
D000939:Epitopes; D007519:Isoantigens; D012794:Sialic Acids