The enzymatic preparation of [alpha-32P]ATP, [alpha-32P]GTP, [32P]cAMP, and [32P]cGMP, and their use in the assay of adenylate and guanylate cyclases and cyclic nucleotide phosphodiesterases.
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000032:analysis; D015106:3',5'-Cyclic-GMP Phosphodiesterases / Q000032:analysis; D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D000262:Adenylyl Cyclases / Q000032:analysis*; D002474:Cell-Free System; D000242:Cyclic AMP / Q000096:biosynthesis; D006152:Cyclic GMP / Q000096:biosynthesis; D006160:Guanosine Triphosphate / Q000096:biosynthesis*; D006162:Guanylate Cyclase / Q000032:analysis*; D008722:Methods; D009712:Nucleotides, Cyclic / Q000096:biosynthesis*; D010727:Phosphoric Diester Hydrolases / Q000032:analysis*; D010761:Phosphorus Radioisotopes
D009712:Nucleotides, Cyclic; D010761:Phosphorus Radioisotopes; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D000242:Cyclic AMP; D010727:Phosphoric Diester Hydrolases; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D015106:3',5'-Cyclic-GMP Phosphodiesterases; D000262:Adenylyl Cyclases; D006162:Guanylate Cyclase; D006152:Cyclic GMP