The fate of IgE bound to rat basophilic leukemia cells.
The present study investigates the fate of the cell-bound IgE by using a well-characterized rat basophilic leukemia cell line and a purifed IgE myeloma protein. Both histamine-releasing and nonreleasing cell lines were examined. In both cases, no evidence for cell-mediated IgE catabolism could be elicited. Both the dissociated IgE and the receptors remained intact for prolonged periods of time, as demonstrated by binding assays. Internalization and/or recycling of membrane-bound IgE could not be demonstrated by E. M. autoradiography. We found only limited time-dependent changes in accessibility to anti-IgE antibody, trypsin, or elution at low pH (2.9 to 3.1). A biphasic dissociation of cell-bound 125I-IgE during incubation in the presence of excess unlabeled IgE was reproducibly observed; the more slowly dissociated IgE was also less readily dissociated at pH 3.4. These studies lead us to conclude that, in vitro, IgE resides in a functional orientation on the surface of RBL-1 cells, for prolonged periods of time.
D000818:Animals; D001345:Autoradiography; D001491:Basophils / Q000276:immunology*; D001666:Binding Sites, Antibody; D002471:Cell Transformation, Neoplastic; D006863:Hydrogen-Ion Concentration; D007073:Immunoglobulin E / Q000276:immunology*; D007158:Immunologic Techniques; D007942:Leukemia, Experimental / Q000276:immunology*; D011817:Rabbits; D051381:Rats; D013997:Time Factors; D014357:Trypsin / Q000494:pharmacology
D007073:Immunoglobulin E; D014357:Trypsin