[Immobilization of Penicillium vitale glucose-oxidase on aminosilochrome and properties of immobilized enzyme].
Penicillium vitale glucose-oxidase modified by means of the carbohydrate component oxidation is added covalently to aminoorganosylochrome. The activity of the immobilized preparations is 20-38% depending on the protein-carrier ration in immobilization. Comparison of some properties of native and immobilized glucose-oxidase showed that the rH optimum of the immobilized glucose-oxidase is slightly widened towards the alkaline regions; the immobilized glucose-oxidase possesses a considerably higher pH-stability at pH alkaline values; the immobilized glucose-oxidase preparations are characterized by a significantly greater thermostability: their thermoinactivation constant at 65 degrees C is 8-10 times lower than that of the native enzyme.
D004355:Drug Stability; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005949:Glucose Oxidase / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010407:Penicillium / Q000201:enzymology*; D012822:Silicon Dioxide; D013696:Temperature
D004800:Enzymes, Immobilized; D012822:Silicon Dioxide; D005949:Glucose Oxidase
Immobilizatsiia gliukozooksidazy Penicillium vitale na aminosilokhrome i svoĭstva immobilizovannogo fermenta.