Rat cell surface antigens. I. Isolation and partial characterization of an Ag-B antigen.
Rat major histocompatibility or Ag-B antigen was isolated from soluble extracts of lymphoid cells by affinity chromatography. The purified antigen was shown in SDS gel electrophoresis to have a molecular weight of 30,000 to 35,000 daltons. In addition, amino acid composition was found to resemble, generally, that of HL-A and H-2 antigens. It thus appears that the demonstrated homology between H-2 and HL-A extends also to Ag-B.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002462:Cell Membrane / Q000276:immunology; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes; D006649:Histocompatibility Antigens / Q000302:isolation & purification*; D007457:Iodine Radioisotopes; D008214:Lymphocytes / Q000276:immunology*; D008970:Molecular Weight; D051381:Rats; D011919:Rats, Inbred Strains / Q000276:immunology*
D000596:Amino Acids; D000939:Epitopes; D006649:Histocompatibility Antigens; D007457:Iodine Radioisotopes