Antiglobulin factors in normal mouse serum reacting with enzyme-digested gamma globulin.
The presence of antiglobulin factors in normal mouse serum was studied. Mice were injected with human 0 Rh(+) erythrocytes and the resulting gamma globulins were digested with papain. The reaction was demonstrated by agglutinations of human 0 Rh (+) perythryocytes sensitized with the Fab fragments when treated with normal mouse serum. Studies suggested that there are two factors in mice, a 7S AND A 19S globulin form. Cross-reactivity between strains of mice was demonstrated.
D000017:ABO Blood-Group System; D000645:Ammonium Sulfate; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis*; D000937:Antigen-Antibody Reactions; D000941:Antigens; D001798:Blood Proteins / Q000032:analysis; D003429:Cross Reactions; D004912:Erythrocytes / Q000276:immunology; D006386:Hemagglutination Tests; D006801:Humans; D007106:Immune Sera; D007122:Immunoelectrophoresis; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G / Q000032:analysis; D008623:Mercaptoethanol; D051379:Mice; D008810:Mice, Inbred C57BL; D010206:Papain; D011817:Rabbits / Q000276:immunology; D012204:Rh-Hr Blood-Group System; D014461:Ultracentrifugation; D005719:gamma-Globulins
D000017:ABO Blood-Group System; D000888:Antibodies, Anti-Idiotypic; D000941:Antigens; D001798:Blood Proteins; D007106:Immune Sera; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D012204:Rh-Hr Blood-Group System; D005719:gamma-Globulins; D008623:Mercaptoethanol; D010206:Papain; D000645:Ammonium Sulfate