[The influence of cytochalasin B, colchicine, and vinblastine on the attachment of Entamoeba histolytica to glass surfaces (author's transl)].
The attachment of E. histolytica trophozoites from monoxenic TPS-medium to glass surfaces was studied at different temperatures and pH values and in the presence of cytochalasin B, colchicine, and vinblastine. At pH 7.0 and at 36 degrees C optimal adhesion rates of the amebae were observed in the range from 80% to 95%. Marked inhibition of adhesion was noted at higher concentrations of cytochalasin B. The adhesion rate was reduced to 50% by 50 micrograms/ml CB. Higher dosages successively prevented nearly all adhesion. Colchicine was without any effect. Vinblastine had a certain low effect but only at very high dosage. The observations are consistent with the assumption that the adhesion process is at least partially mediated by cytochalasin-sensitive contractile microfilaments, whereas microtubuli are obviously not involved in the cell-substrate adhesion.
D000268:Adhesiveness; D000818:Animals; D003078:Colchicine / Q000494:pharmacology*; D003571:Cytochalasin B / Q000494:pharmacology*; D004748:Entamoeba histolytica / Q000187:drug effects* / Q000502:physiology; D005898:Glass; D006863:Hydrogen-Ion Concentration; D013696:Temperature; D014747:Vinblastine / Q000494:pharmacology*
D003571:Cytochalasin B; D014747:Vinblastine; D003078:Colchicine
Der Einfluss von Cytochalasin B, Colchicin und Vinblastin auf die Adhäsion von Entamoeba histolytica an Glasoberflächen.