Reverse transciptase activity associated with R-type virus-like particles of SV40-transformed hamster cells (TSV5 clone 2).
A line of hamster cells transformed by SV40 (TSV5 clone 2) contains different DNA polymerase activities. One of them has the same template specificity as viral reverse transcriptases. Partial isolation of R-type virus-like particles from TSV5 clone 2 shows that a reverse transcriptase activity is associated with these RNA viruses. This reverse transcriptase was partially purified and its biochemical properties are described.
D000818:Animals; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002471:Cell Transformation, Neoplastic; D002474:Cell-Free System; D002999:Clone Cells; D006224:Cricetinae; D004254:DNA Nucleotidyltransferases / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology; D004721:Endoplasmic Reticulum / Q000382:microbiology; D011119:Polynucleotides / Q000378:metabolism; D012328:RNA Viruses / Q000201:enzymology* / Q000648:ultrastructure; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D013539:Simian virus 40 / Q000254:growth & development; D013698:Templates, Genetic
D011119:Polynucleotides; D003907:Dexamethasone; D004254:DNA Nucleotidyltransferases; D012194:RNA-Directed DNA Polymerase; D001973:Bromodeoxyuridine