Demonstration of 8 S-cytoplasmic oestrogen receptor in rat mullerian duct.
1. High affinity macromolecular binding of the non-steroidal synthetic oestrogen [3H]diethylstilboestrol and of [3H] oestradiol-17beta in cytosol of Müllerian duct and uterus, and in blood plasma of perinatal rats, was investigated by sucrose density gradient sedimentation. 2. While [3H] oestradiol was bound to both the characteristic 8 S uterine cytoplasmic receptor and a 4 S component of uterine cytosol and plasma of 11-day-old rats, [3H] diethylstilboestrol was bound almost exclusively by the 8 S cytoplasmic receptor. 3. The greatly reduced binding of [3H] diethylstilboestrol to the 4 S plasma plasmic receptor in the Müllerian duct (precursor of the uterus) of 20-day-old foetuses.
D000818:Animals; D001665:Binding Sites; D001798:Blood Proteins / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D004054:Diethylstilbestrol / Q000378:metabolism*; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D005333:Fetus; D009095:Mullerian Ducts / Q000378:metabolism*; D009124:Muscle Proteins / Q000378:metabolism; D011247:Pregnancy; D011485:Protein Binding; D051381:Rats; D011956:Receptors, Cell Surface; D014599:Uterus / Q000378:metabolism; D000509:alpha-Fetoproteins / Q000378:metabolism
D001798:Blood Proteins; D009124:Muscle Proteins; D011956:Receptors, Cell Surface; D000509:alpha-Fetoproteins; D004958:Estradiol; D004054:Diethylstilbestrol