Biochemical characterization of putative subviral particulates from human malignant breast tumors.
Particulates with the properties of cores and/or ribonucleoproteins of RNA tumor viruses have been isolated from Sterox-SL-treated fractions of murine and human mammary adenocarcinomas. These particulates have an RNA-directed DNA polymerase, a 60 to 70 S RNA, and a density of 1.26 g/ml or greater in sucrose equilibrium density gradients. Their uniquely higher densities lead to banding in regions comparatively free of cellular contaminants. These circumstances minimize some of the technical complications of performing the simultaneous detection assay in the presence of cell debris.
D000230:Adenocarcinoma / Q000382:microbiology*; D000818:Animals; D001943:Breast Neoplasms / Q000382:microbiology*; D002458:Cell Fractionation; D002499:Centrifugation, Density Gradient; D005260:Female; D006801:Humans; D008325:Mammary Neoplasms, Experimental / Q000382:microbiology; D051379:Mice; D009858:Oncogenic Viruses / Q000302:isolation & purification / Q000378:metabolism*; D012328:RNA Viruses / Q000302:isolation & purification / Q000378:metabolism*; D012367:RNA, Viral / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism
D012367:RNA, Viral; D012194:RNA-Directed DNA Polymerase