Binding of mercurials to membrane suspensions and undenatured proteins.
Binding capacities of membrane suspensions and dissolved compounds for mercurials were titrated by a new potentiometric method. Critical steps included a silver electrode of new design, the use of L-cysteine as a thiol buffer, a nitrogen atmosphere, and pretreatment of samples with equimolar mercurial and cysteine. Titrations had a sharp endpoint, accurate +/- 26 nmole methylmercury or +/- 8 nmole mercuric salt. Measurements of binding capacity of bovine serum albumin averaged 93% of the titer predicted for one SH group per molecule; those of human hemoglobin yielded 86-91% of the titer predicted for two SH groups per molecule. Yields dropped with exposure of protein solutions or membrane suspensions to atmospheric oxygen. Brain microsomes had significantly higher binding capacities (per milligram of protein) than red blood cell ghosts. The ratio of endpoint titers of CH3HgCl to HgCl2 averaged 2:1 in assays of cysteine, proteins, and membranes, showing that the assay was free of denaturation artifacts and protein-protein interference. Solutions of EDTA showed measurable binding of Hg2+ but not of CH3Hg+. Satisfactory titrations were also obtained with N-ethylmaleimide.
D000818:Animals; D001921:Brain / Q000648:ultrastructure; D002462:Cell Membrane / Q000378:metabolism*; D004566:Electrodes; D004910:Erythrocyte Membrane / Q000378:metabolism; D017669:Mercury Compounds / Q000378:metabolism*; D008861:Microsomes / Q000378:metabolism; D011199:Potentiometry; D011489:Protein Denaturation; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000378:metabolism
D017669:Mercury Compounds; D011506:Proteins; D013438:Sulfhydryl Compounds; D012710:Serum Albumin, Bovine