A mouse plasma substance carrying beta2-microglobulin activity and lacking in H-2 alloantigenic activity.
A mouse plasma substance carrying beta-2-microglobulin activity and lacking in H-2 alloantigenic activity was separated from the blood plasma of A/J strain female mice. The plasma substance had a molecular size of about 300,000-400,000 daltons and an electrophoretic mobility of alpha-globulin. The plasma substance was split by papain digestion to a fragment of about 50,000-60,000 daltons that still carried beta2-microglobulin activity. The papain-split plasma substance was devoid of H-2 alloantigenic activities and Thy-1 and TL alloantigenic activities as well, but yet had a two-component structure that was similar to papain-solubilized H-2 molecules. It contained a 37,000-dalton component linked non-covalently to an 11,00-dalton component, i.e. mouse beta2-microglobulin. This plasma substance appears to be different from Ss protein or Slp protein, both found in mouse serum.
D000818:Animals; D001609:Beta-Globulins / Q000032:analysis*; D001798:Blood Proteins / Q000276:immunology*; D005260:Female; D006680:HLA Antigens; D006649:Histocompatibility Antigens; D051379:Mice; D008805:Mice, Inbred A; D008970:Molecular Weight; D011487:Protein Conformation; D001613:beta 2-Microglobulin / Q000032:analysis*
D001609:Beta-Globulins; D001798:Blood Proteins; D006680:HLA Antigens; D006649:Histocompatibility Antigens; D001613:beta 2-Microglobulin