[Isolation of beta2-microglobulin from human colostrum (author's transl)].
The beta2-microglobulin from human colostrum was purified by a combination of ordinary protein-chemical techniques: gel filtration, ion-exchange chromatography and zone electrophoresis. The procedure is organized in such a way that the simultaneous isolation of many other milk proteins is possible. The beta2-microglobulin obtained from colostrum cannot be distinguished by physical-chemical or immunological means from the beta2-microblobulin isolated from the urine of patients with kidney-tubule diseases. At the beginning of lactation, human milk contains significantly more than 10 mg/-100 ml beta2-microglobulin, but the concentration drops within two or three days to 15-30% of the original amount.
D001609:Beta-Globulins / Q000302:isolation & purification*; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D003126:Colostrum / Q000032:analysis*; D004586:Electrophoresis; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007106:Immune Sera; D005779:Immunodiffusion; D007525:Isoelectric Focusing; D007774:Lactation; D011247:Pregnancy; D013997:Time Factors; D014461:Ultracentrifugation; D014462:Ultrafiltration; D001613:beta 2-Microglobulin / Q000276:immunology / Q000302:isolation & purification* / Q000652:urine
D001609:Beta-Globulins; D007106:Immune Sera; D001613:beta 2-Microglobulin
Isolierung von beta2-Mikroglobulin aus Human-Kolostrum