Quantitative assay for algal chemotaxis.
A quantitative capillary assay is described for measuring chemoreception in the neritic and littoral unicellular alga Dunaliella tertiolecta. Lucite chemotaxis plates were used in the assay with 3-microliter capillaries. A Coulter Counter was employed to determine algal cell numbers. D. tertiolecta is attracted to ammonium ion with a maximum positive response at 10(-3) M. Inclusion of calcium and L-methionine in the chemotaxis medium stimulates algal chemoreception, although neither chemical is essential for motility. Attraction of the chlorophyte to ammonium is dependent on time of incubation, cell density, and pH. The optimum pH for attraction was found to be 6.25.
D000643:Ammonium Chloride / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D002633:Chemotaxis / Q000187:drug effects; D003470:Culture Media; D056890:Eukaryota / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D008715:Methionine / Q000494:pharmacology; D008828:Microbiological Techniques; D012623:Seawater; D014871:Water Microbiology
D003470:Culture Media; D000643:Ammonium Chloride; D008715:Methionine; D002118:Calcium