[Growth and lipopolysaccharide content of salmonellae grown in submersed cultures according to the batch-method. 2. Communication: influence of growth phases on lipopolysaccharide synthesis (author's transl)].
The paper describes cultivations of 4 Salmonella S-forms and 1 SR mutant, performed in complex medium under constant conditions of temperature, pH and aeration. The experiments show that lipopolysaccharide (LPS) biosynthesis underlies quantitative differences with the growth phases, resulting in changes in the LPS content of the cell masses. During the exponential phase a decline takes place in the percentage of LPS contained by the 4 S-forms. In addition, in the phase of delayed growth acceleration, 3 of these strains exhibit temporary, complete stagnation in LPS formation. When the cultures enter the stationary phase, LPS biosynthesis also discontinues. The SR-mutant differs from the S-forms especially in that the rate of LPS synthesis and with it, the percent lipopolysaccharide content of the cells, increase greatly in the exponential growth phase. The causes and effects of the changes observed are discussed.
D002455:Cell Division; D003470:Culture Media; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007106:Immune Sera; D008070:Lipopolysaccharides / Q000032:analysis / Q000096:biosynthesis*; D008954:Models, Biological; D010100:Oxygen / Q000378:metabolism; D011312:Pressure; D012475:Salmonella / Q000254:growth & development* / Q000378:metabolism; D012477:Salmonella enteritidis / Q000254:growth & development / Q000378:metabolism; D012486:Salmonella typhimurium / Q000254:growth & development / Q000378:metabolism; D013194:Staining and Labeling; D013997:Time Factors
D003470:Culture Media; D007106:Immune Sera; D008070:Lipopolysaccharides; D005947:Glucose; D010100:Oxygen
Wachstum und Lipopolysaccharidgehalt von Salmonellen bei Submerszüchtungen nach dem Batch-Verfahren. 2. Mitteilung: Einfluss der Wachstumsphase auf die Lipopolysaccharidsynthese