RNA-polymerase activity in isolated nuclei of developing silkworm embryos.
Nuclei isolated from developing embryos of the silkworm (Bombyx mori) are capable of RNA synthesis in vitro. The incorporation of C-14-uridine triphosphate (C-14-UTP) into the acid-insoluble fraction requires the presence of the other three ribonucleoside triphosphates; it is inhibited by actinomycin, olivomycin, and ethidium bromide, and is stimulated by ammonium sulfate. In a medium of moderate ionic strength C-14-UTP incorporation is slightly inhibited by alpha-amanitine (4 mug/ml), whereas in the presence of 0.2 M ammonium sulfate the same alpha-amanitine concentration reduces the incorporation by 90%. These data indicate that silkworm embryo nuclei, like nuclei from several other sources, contain two different RNA-polymerase fractions.
D000546:Amanitins / Q000494:pharmacology; D000645:Ammonium Sulfate / Q000494:pharmacology; D000818:Animals; D012831:Bombyx / Q000196:embryology* / Q000201:enzymology; D002250:Carbon Radioisotopes; D002467:Cell Nucleus / Q000187:drug effects / Q000201:enzymology*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D003609:Dactinomycin / Q000494:pharmacology; D004996:Ethidium / Q000494:pharmacology; D009848:Olivomycins / Q000494:pharmacology; D012313:RNA / Q000096:biosynthesis; D013698:Templates, Genetic; D014500:Uracil Nucleotides / Q000378:metabolism
D000546:Amanitins; D002250:Carbon Radioisotopes; D014500:Uracil Nucleotides; D003609:Dactinomycin; D009848:Olivomycins; D012313:RNA; D012321:DNA-Directed RNA Polymerases; D004996:Ethidium; D000645:Ammonium Sulfate