Ultrastructure of lipopolysaccharide isolated from Thermoplasma acidophilum.
The fine structure of lipopolysaccharide isolated from Thermoplasma acidophilum was examined by electron microscopy. Negative staining of the lipopolysaccharide revealed long, ribbon-like structures with some branching. The average width of the lipopolysaccharide ribbons was 5 nm. Treatment of the lipopolysaccharide with 0.5% sodium dodecyl sulfate resulted in the dissociation of the ribbon-like structures to spherical- and vesicular-shaped particles and some short, rodlike structures. Results suggest that the lipopolysaccharide from T. acidophilum is morphologically similar to lipopolysaccharide isolated from gram-negative bacteria.
D008070:Lipopolysaccharides / Q000302:isolation & purification; D008854:Microscopy, Electron; D009174:Mycoplasma / Q000648:ultrastructure*; D010772:Phosphotungstic Acid; D011135:Polysaccharides, Bacterial / Q000302:isolation & purification; D012967:Sodium Dodecyl Sulfate; D013194:Staining and Labeling
D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D010772:Phosphotungstic Acid; D012967:Sodium Dodecyl Sulfate