Effect of anti-beta2-microglobulin on antigen and allogeneic lymphocyte-induced proliferation of human lymphocytes.
The effect of anti-beta2-microglobulin (beta2m) on the mixed lymphocyte reaction (MLR), and an antigen-induced proliferative response was studied. Anti-beta2m IgG and Fab' fragments completely inhibited the MLR. Preincubation of stimulator or responder cells with anti-beta2m suggested that the major effect of anti-beta2m may be on the responder cell population. A clear-cut effect on responder cells was demonstrated by showing that anti-beta2m completely inhibited a MLR in which the stimulator population was a beta2m negative lymphoblastoid cell line. Anti-beta2m also inhibited PPD-induced proliferation of sensitized lymphocytes. The kinetics of this inhibition indicated that anti-beta2m added within the first 18 hr of stimulation was effective in inhibiting the proliferative response. These data are discussed in light of the hypothesis that beta2m may be a subunit of an antigen receptor on T cells.
D000818:Animals; D000941:Antigens; D001609:Beta-Globulins / Q000494:pharmacology*; D002051:Burkitt Lymphoma; D002460:Cell Line; D002478:Cells, Cultured; D006801:Humans; D007106:Immune Sera; D007700:Kinetics; D008213:Lymphocyte Activation / Q000187:drug effects*; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000276:immunology*; D011817:Rabbits / Q000276:immunology; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014373:Tuberculin
D000941:Antigens; D001609:Beta-Globulins; D007106:Immune Sera; D014373:Tuberculin; D014316:Tritium; D013936:Thymidine