An evaluation of some complexing methods for the histochemistry of calcium.
A selection of reagents capable of complexing with calcium in various ways was compared by applying them to tissue sections known to contain calcium in a variety of physiochemical states. Their histochemical potential was evaluated according to their sensitivity, specificity, accuracy of localisation and intensity of staining. Murexide, for light microscopy, and 8-hydroxyquinoline, for fluorescence microscopy proved to be the best overall reagents. They failed to demonstrate calcium oxalate, which was well shown by naphthalhydroxamic acid.
D002118:Calcium / Q000032:analysis*; D002614:Chelating Agents; D006651:Histocytochemistry; D006801:Humans; D010070:Oxalates / Q000032:analysis; D013194:Staining and Labeling
D002614:Chelating Agents; D010070:Oxalates; D002118:Calcium