In vitro induction and measurement of hemolytic plaque forming cells in man.
Following co-cultivation with sheep red cells or ovalbumin, Hypaque-Ficoll-separated human tonsillar lymphocytes were demonstrated to generate specific hemolytic PFC with maximum numbers at day 5-7. PFC were enumerated on poly-L-lysine coupled red cell monolayers in Microtest-II-plates. Plaque formation appeared to be puromycin-sensitive, complement-dependent and showed clear specificity for the antigen present during the inductive culture. Treatment of PFC with mu-chain specific antisera and complement resulted in complete inactivation of PFC; gamma-chain antisera had no effect. The development of such a simple and sensitive assay system permits the analysis of cellular interactions required for the induction of PFC responses in man.
D000888:Antibodies, Anti-Idiotypic; D000921:Antibody-Producing Cells / Q000276:immunology*; D003165:Complement System Proteins / Q000378:metabolism; D000939:Epitopes; D006462:Hemolytic Plaque Technique / Q000379:methods*; D006801:Humans; D008934:Mitogens / Q000494:pharmacology; D011691:Puromycin / Q000494:pharmacology
D000888:Antibodies, Anti-Idiotypic; D000939:Epitopes; D008934:Mitogens; D011691:Puromycin; D003165:Complement System Proteins