[A simple gas chromatographic method for the determination of hypnotics in the serum (author's transl)].
In the extract of the serum, prepared as described by Külpmann (1979) (this J. 17, 89-96), other hypnotics, in addition to the barbiturates can be determined by gas chromatography; these are: carbromal, 2,2-diethylallylacetamide, ethinamate, glutethimide, methyprylone and pyrithyldione. Methaqualone can be detected qualitatively. The coefficient of variation for the precision in the series is dependent on the hypnotic investigated and ranges from 2.1 to 8.5%, the recovery from 76 to 92%; the detection limit is estimated to be 1.6 up to 4.6 mumol/l. The specificity was proved by comparison 1) of analyses of sera from poisoned patients or animals before and after the additional purification of the extract by thin-layer chromatography, 2) with the retention times of about 100 drugs under the gas chromatographic conditions that were used. The method allows the determination of 18 barbiturates and 7 non-barbiturates within one to two hours.
D002219:Carbamates / Q000097:blood; D002849:Chromatography, Gas / Q000379:methods; D005984:Glutethimide / Q000097:blood; D006801:Humans; D006993:Hypnotics and Sedatives / Q000097:blood*; D010881:Piperidones / Q000097:blood; D014508:Urea / Q000097:blood
D002219:Carbamates; D006993:Hypnotics and Sedatives; D010881:Piperidones; D014508:Urea; D005984:Glutethimide
Eine einfache gaschromatographische Methode zur Bestimmung von Schlafmitteln im Serum.