Presence of blood group H antigen on a carcinoembryonic antigen, and its enzymatic modification into blood group A and B specificities.
A carcinoembryonic antigen (CEA-M) was purified from a hepatic metastasis obtained from a blood group O patient with cancer of the rectum. Using 125I-labeled carcinoembryonic antigen (CEA) and blood group antisera, H specificity has been found on the CEA-M. As the addition of anti-H to anti-CEA does not modify the extent of binding of labeled CEA-M to its antibodies (86%), the H and CEA determinants are carried by the same molecule. The affinity chromatography of CEA-M on an immunosorbent "anti-H-Sepharose" demonstrated that a proportion of CEA-M molecules might bear both H and CEA antigenic determinants. In addition, glycosyltransferases were used to modify the blood group H specificity into blood group A or B specificities.
D000017:ABO Blood-Group System; D000951:Antigens, Neoplasm; D001665:Binding Sites; D002272:Carcinoembryonic Antigen; D002846:Chromatography, Affinity; D000939:Epitopes; D005690:Galactose / Q000378:metabolism; D005700:Galactosyltransferases / Q000494:pharmacology; D006801:Humans; D007106:Immune Sera; D014531:Uridine Diphosphate Galactose / Q000494:pharmacology; D014536:Uridine Diphosphate N-Acetylgalactosamine / Q000494:pharmacology
D000017:ABO Blood-Group System; D000951:Antigens, Neoplasm; D002272:Carcinoembryonic Antigen; D000939:Epitopes; D007106:Immune Sera; D014531:Uridine Diphosphate Galactose; D014536:Uridine Diphosphate N-Acetylgalactosamine; D005700:Galactosyltransferases; D005690:Galactose