Binding of human IgE immunoglobulin to rat mast cells. A study by means of three independent techniques.
The binding of human myeloma IgE immunoglobulin on rat mast cells was studied by three independent techniques. A mixed agglutination reaction with anti-IgE-coated Sephadex granules demonstrated that only human IgE-coated rat mast cells were clearly agglutinated. This binding is strong (50% agglutination) in 3 min and progresses for 30 min (95% agglutination). Autoradiographic studies with 125I-labelled human serum proteins demonstrated the selective formation of grains on mast cells incubated with labelled IgE. Upon action of anti-IgE antiserum on IgE-coated rat mast cells, the mast cells released up to 47.5% of their total histamine content in a fluorometric histamine assay. A relationship was established between sensitizing doses of human IgE and histamine release. These results bring evidence for a binding of human IgE on rat mast cells and imply the existence of receptors for this immunoglobulin on mast cell membrane.
D000818:Animals; D001345:Autoradiography; D001666:Binding Sites, Antibody; D003594:Cytoplasmic Granules; D005260:Female; D006386:Hemagglutination Tests; D006636:Histamine Release; D006801:Humans; D007104:Immune Adherence Reaction; D007073:Immunoglobulin E; D007074:Immunoglobulin G / Q000302:isolation & purification; D007075:Immunoglobulin M / Q000302:isolation & purification; D007457:Iodine Radioisotopes; D008214:Lymphocytes / Q000276:immunology; D008407:Mast Cells / Q000276:immunology*; D009101:Multiple Myeloma / Q000276:immunology; D009194:Myeloma Proteins; D051381:Rats; D013154:Spleen / Q000166:cytology
D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007457:Iodine Radioisotopes; D009194:Myeloma Proteins; D007073:Immunoglobulin E