Reduced nicotinamide adenine dinucleotide dependent reduction of fumarate coupled to membrane energization in a cytochrome deficient mutant of Escherichia coli K12.
Escherichia coli SASX76 does not form cytochromes unless supplemented with 5-aminolevulinic acid. It can grow anaerobically on glycerol and DL-glycerol 3-phosphate in the absence of 5-aminolevulinic acid with fumarate but not with nitrate as the terminal electron acceptor. Cytochrome-independent NADH oxidase, glycerol 3-phosphate- and NADH-fumarate oxidoreductase activities are induced by anaerobic growth on a glycerol-fumarate medium. The pathway of electrons from substrate to fumarate involves menaquinone. The NADH-fumarate oxidoreductase and cytochrome-independent NADH oxidase systems are inhibited by piericidin A, 2-heptyl-4-hydroxyquinoline N-oxide, and iron chelating agents. Both systems can energize the membrane particles as indicated by quenching of atebrin fluorescence.
D000622:Aminolevulinic Acid / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism*; D002614:Chelating Agents / Q000494:pharmacology; D003580:Cytochromes / Q000378:metabolism*; D001728:Dicumarol / Q000494:pharmacology; D004926:Escherichia coli / Q000378:metabolism*; D005650:Fumarates / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D005994:Glycerophosphates / Q000378:metabolism; D006912:Hydroxyquinolines / Q000494:pharmacology; D009243:NAD / Q000494:pharmacology*; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D009566:Nitrates / Q000378:metabolism; D010880:Piperidines / Q000494:pharmacology; D011809:Quinones / Q000378:metabolism; D013050:Spectrometry, Fluorescence
D002614:Chelating Agents; D003580:Cytochromes; D005650:Fumarates; D005994:Glycerophosphates; D006912:Hydroxyquinolines; D009566:Nitrates; D010880:Piperidines; D011809:Quinones; D009243:NAD; D001728:Dicumarol; D000622:Aminolevulinic Acid; D005993:Glycerolphosphate Dehydrogenase; D009247:NADH, NADPH Oxidoreductases; D005990:Glycerol