Plastic sectioning for light microscopy of Pyrenomycetes.
In preparation for light microscopy, ascocarps of Sordaria fimicola Ces. & DeNot. were embedded in Spurr's medium and sectioned at 1-1.5 mum on an ultramicrotome. Sections were floated on Giemsa staining solution at 60 C for 10-30 min, washed in distilled water, affixed to slides by drying, and mounted in immersion oil. Best preservation of the delicate sterile tissues of the centrum was obtained by fixation in 1% KMnO4 for 2.5-3 hr, followed by the Giemsa stain. This method is suggested for future studies on the morphology of perithecial ascomycetes.
D001203:Ascomycota / Q000648:ultrastructure*; D003584:Cytological Techniques; D008867:Microtomy; D010232:Paraffin; D010969:Plastics; D013194:Staining and Labeling
D010969:Plastics; D010232:Paraffin