The reaction of cow beta-lactoglobulin with tetracyanoaurate(III).
The reaction of cow beta-lactoglobulin with Au(CN)-4 is shown to label the free sulphydryl group on the protein by a reductive mechanism yielding a stable complex. High salt concentrations are shown to affect the pH-dependent conformational transition (N in equilibrium R yields S). It is hoped that Au(CN)-4 may be of more widespread use as a specific heavy-atom label for the isomorphous replacement method.
D000818:Animals; D002417:Cattle; D003485:Cyanates; D003486:Cyanides; D004355:Drug Stability; D006046:Gold; D017612:Gold Compounds; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007782:Lactoglobulins; D008892:Milk; D009942:Organometallic Compounds; D011485:Protein Binding; D011487:Protein Conformation
D003485:Cyanates; D003486:Cyanides; D017612:Gold Compounds; D007782:Lactoglobulins; D009942:Organometallic Compounds; C020808:tetracyanoaurate(III); D006046:Gold