title: Induction of mitosis in mature neurons in central nervous system by sustained depolarization.
abstract: DNA synthesis and mitosis have been induced in vitro in fully differentiated neurons from the central nervous system by depolarization with a variety of agents that produce a sustained rise in the intracellular sodium ion concentration and a decrease in the potassium ion concentration. Depolarization was followed in less than 1 hour by an increase in RNA synthesis and in 3 hours by initiation of DNA synthesis. Apparently normal nuclear mitosis ensued, but cytokinesis was not completed in most cells; this resulted in the formation of binucleate neurons. The daughter nuclei each contained the same amount of DNA as the diploid preinduction parental neurons; this implies that true mitogenic replication was induced.
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D006096:Gramicidin / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects; D008938:Mitosis / Q000187:drug effects; D009474:Neurons / Q000378:metabolism / Q000502:physiology*; D010042:Ouabain / Q000494:pharmacology; D012313:RNA / Q000096:biosynthesis; D012965:Sodium Chloride / Q000494:pharmacology; D014701:Veratridine / Q000494:pharmacology
chemical_list: D006096:Gramicidin; D012965:Sodium Chloride; D010042:Ouabain; D012313:RNA; D014701:Veratridine