title: Automated hematoxylin and eosin staining for large volumes of tissue.
abstract: In order to facilitate the large volume of hematoxylin and eosin (H & E) slides generated by 18 experiments from various programs at the National Center for Toxicological Research, a rapid staining method became imperative. Automated staining with the Gam Rad Stainomatic was decided upon using Gill's no. 1 hematoxylin with a 30-minute staining schedule. Presently, 1,080 slides per instrument per day are stained with a good dependable stain. The cost of this H & E staining method is 0.15 cents per slide.
mesh_terms: D001578:Benzopyrans; D005452:Fluoresceins; D006416:Hematoxylin; D006652:Histological Techniques; D013194:Staining and Labeling / Q000295:instrumentation*
chemical_list: D001578:Benzopyrans; D005452:Fluoresceins; D006416:Hematoxylin