Inhibitory effect of kanamycin on evoked transmitter release. Reversal by 3,4-diaminopyridine.
The effect of kanamycin (Kn) on evoked transmitter release was examined in frog end-plates in vitro. By a presynaptic action, Kn (0.02 to 1 mM) significantly reduced the amount of acetylcholine liberated by nerve stimulation. In addition to its presynaptic effects, Kn (0.96 mM) decreased the size of the miniature end-plate potentials possibly by acting at the postsynaptic level. 3,4-Diaminopyridine (4.5 microM) reversed the presynaptic effects of Kn but did not modify its postsynaptic action.
D000631:Aminopyridines / Q000494:pharmacology*; D000818:Animals; D001001:Anura; D066298:In Vitro Techniques; D007612:Kanamycin / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D008564:Membrane Potentials / Q000187:drug effects; D009045:Motor Endplate / Q000187:drug effects; D009469:Neuromuscular Junction / Q000187:drug effects; D018377:Neurotransmitter Agents / Q000378:metabolism*; D011893:Rana esculenta; D012584:Sciatic Nerve / Q000187:drug effects / Q000378:metabolism
D000631:Aminopyridines; D018377:Neurotransmitter Agents; D007612:Kanamycin