title: DNA synthesis in vitro by cells from mice immunized with picryl chloride: effect of injection of immune cells.
abstract: Mice were immunized with picryl chloride and the regional nodes taken at various times afterwards. These cells spontanesouly synthesized DNA in vitro as measured by thymidine incorporation over an 18-hour period and the peak incorporation occurred when the cells were takin on day 3. When the mice were injected with cells taken 5 days after immunization with picryl chloride and then immunized, there was a depression of the spontaneous DNA synthesis in vitro. This was absent on day 2, most marked on day 3 and still present on day 4. Cells from donors immunized with 4-ethoxymethylene-2-phenyloxazolone had a smaller but definite effect. Attempts to reproduce the phenomenon by in vitro mixtures of cells taken at various times after immunization in vivo were unsuccessful.
mesh_terms: D000818:Animals; D004247:DNA / Q000096:biosynthesis*; D000939:Epitopes; D005260:Female; D007165:Immunosuppression Therapy; D066298:In Vitro Techniques; D008198:Lymph Nodes / Q000276:immunology*; D017710:Lymphocyte Transfusion; D051379:Mice; D008808:Mice, Inbred CBA; D010853:Picryl Chloride / Q000276:immunology*; D014184:Transplantation, Homologous
chemical_list: D000939:Epitopes; D004247:DNA; D010853:Picryl Chloride