[Effect of triterpene glycosides on plasma membrane permeability for UV-absorbing substances in Saccharomyces carlsbergensis yeast cells].
The effect of triterpene glycosides of cauloside C from Caulophyllum robustum M, stichoposide A from Stichopus japonicus S. and theasaponine from Thea sinensis Z. on permeability of plasmic membranes of Saccharomyces carlsbergensis for UV-absorbing substances was studied. It was found that incorporation of 14C-uridine from the endocellular pool into the yeast acid-insoluble fraction decreased under the effect of the triterpene glycosides as a result of the precursor leakage from the cell into the medium. It was found that the triterpene glycosides stimulated the leakage of the UV-absorbing substances with an absorption maximum at 260 nm from the cells. The maximum membranotropic effect was observed at 30--40 degrees C and in the presence of monovalent potassium, sodium, ammonium and lithium ions in the medium. Cauloside C and theasaponine, pentacyclic glycosides had the highest effect on the permeability at pH 4.8--5.6, while stichoposide A, a tetracyclic glycoside, had the highest effect at pH 7.0.
D002463:Cell Membrane Permeability / Q000187:drug effects*; D003470:Culture Media; D003500:Cyclization; D006027:Glycosides / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D012440:Saccharomyces / Q000187:drug effects* / Q000378:metabolism; D013696:Temperature; D013997:Time Factors; D014315:Triterpenes / Q000494:pharmacology*; D014466:Ultraviolet Rays
D003470:Culture Media; D006027:Glycosides; D014315:Triterpenes
Vliianie nekotorykh triterpenovykh glikozidov na pronitsaemost' plazmaticheskikh membran dlia UF-pogloshchaiushchikh veshchestv v drozhzhevykh kletkakh Saccharomyces carlsbergensis.