title: Effect of cyclic guanosine 3',5'-monophosphate on nitrogen fixation in Rhizobium japonicum.
abstract: The addition of exogenous cyclic guanosine 3',5'-monophosphate (cGMP) at a concentration of 0.1 mM to a free-living culture of Rhizobium japonicum 3I1b110 was found to completely inhibit the expression of nitrogenase activity and markedly inhibit the expression of hydrogenase and nitrate reductase activities. The effect was specific for cGMP. Experiments on the in vivo incorporation of radioactive methionine and subsequent analysis of the labeled proteins on polyacrylamide gels showed that the biosynthesis of nitrogenase polypeptides was inhibited. It appears that the time of addition of cGMP is important since the effect was only seen during the early stages of nif gene expression. The intracellular level of cGMP was found to respond to physiological changes in the cell, and there was a fall in cGMP concentrations when nitrogenase was induced. Microaerophilic-aerobic shift experiments showed that intracellular levels increased from 0.25 pmol/mg of cell protein under microaerophilic conditions to 2.6 pmol/mg of cell protein under aerobic conditions, suggesting that the cellular pool size of cGMP may be under redox control.
mesh_terms: D006152:Cyclic GMP / Q000378:metabolism / Q000494:pharmacology*; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism; D006859:Hydrogen; D008291:Malate Dehydrogenase / Q000378:metabolism; D009565:Nitrate Reductases / Q000378:metabolism; D009586:Nitrogen Fixation / Q000187:drug effects*; D009591:Nitrogenase / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism; D012231:Rhizobium / Q000378:metabolism*
chemical_list: D006859:Hydrogen; D010088:Oxidoreductases; D008291:Malate Dehydrogenase; D009591:Nitrogenase; D009565:Nitrate Reductases; D005974:Glutamate-Ammonia Ligase; D006152:Cyclic GMP