Enzymatic conjugation and hydrolysis of [18O]isoborneol glucuronide.
Introduction of 18O into the acetal linkage of isoborneol glucuronide has been accomplished through the in vitro enzymatic conjugation of 18O-labeled isoborneol by use of immobilized, partially purified, rabbit liver UDP-glucuronyltransferase supplemented with UDP-glucuronic acid. Mass-spectrometric analysis of 18O enrichment in both the aglycon and the intact conjugate made it possible to study both enzymatic conjugation and enzymatic hydrolysis. Analysis by gas chromatography-mass spectrometry indicated that the labeled hydroxyl oxygen of isoborneol is retained during both conjugation and hydrolysis.
D000818:Animals; D001892:Camphanes; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D005965:Glucuronates / Q000378:metabolism*; D014453:Glucuronosyltransferase / Q000378:metabolism*; D006868:Hydrolysis; D066298:In Vitro Techniques; D013058:Mass Spectrometry; D011817:Rabbits; D013729:Terpenes / Q000378:metabolism*
D001892:Camphanes; D005965:Glucuronates; D013729:Terpenes; C021829:isoborneol glucuronide; D014453:Glucuronosyltransferase