Simultaneous demonstration of lipids and starch in plant tissues.
A rapid and easy technique for the simultaneous demonstration of lipids and starch in the same histological section is described. Tissues are prepared by the classical fixing and Araldite M embedding techniques of electron microscopy. Semithin sections are directly stained for 1 hour at 60C with saturated Sudan black B in 70% ethanol without removing the embedding resin. Lipids stain black; starch is shown as white grains contrasting with the blue-grey embedding resin.
D006652:Histological Techniques; D008055:Lipids / Q000032:analysis*; D008854:Microscopy, Electron; D010944:Plants / Q000032:analysis*; D012116:Resins, Plant; D013194:Staining and Labeling / Q000379:methods; D013213:Starch / Q000032:analysis*
D008055:Lipids; D012116:Resins, Plant; D013213:Starch