A radioimmunoassay for bovine parathyroid hormone.
A radioimmunoassay for bovine parathyroid hormone (bPTH) has been developed. An antibody was raised in a goat against 1-84 b PTH which was directed against the carboxy-terminal part of the molecule (no cross-reactivity with synthetic 1-34 b PTH fragment). 1-84 b PTH was labelled with 125I using the chloramine-T method. The tubes were incubated at 4 degrees C for 6 days in an equilibrium system with 25% protein concentration. Separation was performed using plasma-coated charcoal. Jugular venous plasma PTH levels were shown to be increased in hypocalcemic parturient cows.
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D000936:Antigen-Antibody Complex / Q000302:isolation & purification; D002417:Cattle; D000939:Epitopes; D005260:Female; D006996:Hypocalcemia / Q000097:blood; D007457:Iodine Radioisotopes; D010281:Parathyroid Hormone / Q000097:blood* / Q000276:immunology; D011247:Pregnancy; D011863:Radioimmunoassay / Q000379:methods*
D000906:Antibodies; D000936:Antigen-Antibody Complex; D000939:Epitopes; D007457:Iodine Radioisotopes; D010281:Parathyroid Hormone