Verdohemoglobin as a substrate for proteases is applicable to assays over a wide pH range.
Verdohemoglobin, a heme-modified derivative readily obtained by ascorbic acid-coupled oxidation of oxyhemoglobin, was found to be a suitable substrate for protease with which assay can be carried out at all pH values. The advantage of verdohemoglobin over such popular substrates as alkali-and-urea-denatured hemoglobin and casein was demonstrated by a pH-profile study with Pronase E.
D002384:Catalysis; D006454:Hemoglobins; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010108:Oxyhemoglobins; D010447:Peptide Hydrolases / Q000378:metabolism*; D011402:Pronase; D011489:Protein Denaturation; D012995:Solubility; D013379:Substrate Specificity
D006454:Hemoglobins; D010108:Oxyhemoglobins; D010447:Peptide Hydrolases; D011402:Pronase