A rapid and simplified staining of juxtaglomerular granules with aqueous crystal violet.
The method described allows staining of juxtaglomerular granules in a deep purple colour against faint purple background and deep purple erythrocytes. Paraffin sections of mouse kidney fixed in 10% formalin buffered with phosphate are brought to water, stained 3 min in 0.01% crystal violet, rinsed in tap water (or stained 3 min in 0.1% crystal violet in NaHCO3, rinsed in 1% NaHCO3), blotted, differentiated in a 1 : 1 aniline-xylene mixture, cleared in xylene and mounted in a resin.
D000818:Animals; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D005840:Gentian Violet; D007606:Juxtaglomerular Apparatus / Q000648:ultrastructure*; D051379:Mice; D013194:Staining and Labeling
D005840:Gentian Violet