title: Biochemical approach to bovine leukemia.
abstract: Many bovine leukemic lymphocytes produce virus particles when kept in survival cultures in Eagle's Minimum Essential Medium supplemented with 20 percent of inactivated fetal calf serum. Virus particles equilibrate at a density of 1.16 g/ml in sucrose gradients and at a density of 1.12 g/ml in metrizamide gradients. Simultaneous detection tests show that a high molecular weight RNA-reverse transcriptase complex exists in these viruses. Hybridizations between total RNA from bovine leukemic lymphocytes and C-DNA prepared in various known RNA oncogenic viruses show that the virus associated with bovine leukosis is unrelated to RLV, SSV-1, MSV-Ki, FSV-Ga and FLV-Ri.
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis; D007938:Leukemia / Q000382:microbiology / Q000662:veterinary*; D001909:Leukemia Virus, Bovine / Q000201:enzymology / Q000302:isolation & purification; D008214:Lymphocytes / Q000378:metabolism / Q000382:microbiology / Q000648:ultrastructure; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000096:biosynthesis; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D012190:Retroviridae; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral; D012194:RNA-Directed DNA Polymerase