Simultaneous differential staining of nucleic acids, proteins, conjugated proteins and polar lipids by a cationic carbocyanine dye.
The multiple interactions of the cationic carbocyanine dye, 1-ethyl-naptho-[1,2d]thiazolin-2-ylidene)-2-methylpropenyl]naptho[1,2d]thiazolium bromide, 'Stains-described. Many of these substances could be distinguished from one another on the basis of color in conjunction with chemical and enzymatic digestions. Further studies with this dye have shown that under certain conditions polar lipids as well may be distinguished from these substances. Stains-all has a highly sensitive metachromatic reaction for the presence of polar lipids. It is possible to detect the lipids in large part because they are green and contrast with a red- or pink-stained protein background and with the blue-purple of nuclei or cartilage. Where other green substances occur as in sialoglycoproteins of mucous or membranes, the lipids can be distinguished because they are extracted by chloroform-methanol (2:1) or pyridine.
D000273:Adipose Tissue / Q000032:analysis; D000302:Adrenal Cortex / Q000032:analysis; D000313:Adrenal Medulla / Q000032:analysis; D000475:Alkenes; D000818:Animals; D002531:Cerebellum / Q000032:analysis; D004247:DNA / Q000032:analysis*; D006651:Histocytochemistry / Q000379:methods*; D008055:Lipids / Q000032:analysis*; D008168:Lung / Q000032:analysis; D051379:Mice; D011506:Proteins / Q000032:analysis; D011804:Quinolines; D012313:RNA / Q000032:analysis*; D051381:Rats; D012160:Retina / Q000032:analysis; D012584:Sciatic Nerve / Q000032:analysis; D013053:Spectrophotometry; D013194:Staining and Labeling; D001743:Urinary Bladder / Q000032:analysis
D000475:Alkenes; D008055:Lipids; D011506:Proteins; D011804:Quinolines; D012313:RNA; D004247:DNA