Investigation of the system cobalt(II) bovine carbonic anhydrase B-trichloroacetaldehyde.
The interactions between hydrated trichloroacetaldehyde and cobalt(II)bovine carbonic anhydrase B have been investigated as a function of pH by means of electronic spectroscopy of FT nmr spectroscopy. The hydrated aldehyde is bound to the metal ion and its apparent affinity constant is pH dependent with a bell-shaped profile. The kinetic parameters of the dissociation process have also been determined.
D000818:Animals; D002256:Carbonic Anhydrases / Q000097:blood; D002417:Cattle; D002697:Chloral Hydrate; D003035:Cobalt; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D011485:Protein Binding; D013816:Thermodynamics
D003035:Cobalt; D002697:Chloral Hydrate; D002256:Carbonic Anhydrases