Isolation and immunologic characterization of a human. B-lymphocyte-specific, cell surface antigen.
In addition to HL-A antigens, another cell surface protein complex has been obtained from membranes of the human B-lymphoblast cell line IM-1. This complex which was solubilized with papain, consisted of polypeptides of 23,000 and 30,000 daltons (p23, 30). Rabbit antisera to this material precipitated from [35S]methionine-labeled detergent-solubilized cells, three proteins of 39,000, 34,000, and 29,000 daltons. These antisera were specifically cytotoxic for B lymphocytes of peripheral blood, for B-lymphoblast cell lines, and for EAC rosette receptor-positive surface Ig-negative (Null) lymphocytes. The p23,30 complex was not present on T lymphocytes, EAC rosette receptor-negative Null lymphocytes, or platelets. In addition, the p23,30 complex from several cell lines inhibited alloantisera from multiparous Amish women which had been shown to recognize non-HL-A, B-lymphocyte antigens. Some other properties of the anti-p23,30 sera antisera were described.
D000937:Antigen-Antibody Reactions; D001402:B-Lymphocytes / Q000276:immunology*; D001792:Blood Platelets / Q000276:immunology; D002460:Cell Line; D003601:Cytotoxicity Tests, Immunologic; D000939:Epitopes; D006680:HLA Antigens; D006801:Humans; D007519:Isoantigens / Q000032:analysis / Q000302:isolation & purification*; D007959:Lymphocyte Culture Test, Mixed; D008970:Molecular Weight; D011487:Protein Conformation; D012067:Religion; D013499:Surface Properties; D013601:T-Lymphocytes / Q000276:immunology; D001613:beta 2-Microglobulin / Q000276:immunology
D000939:Epitopes; D006680:HLA Antigens; D007519:Isoantigens; D001613:beta 2-Microglobulin