title: Lipoprotein lipase in the fat body of Triatoma brasiliensis.
abstract: Lipoprotein lipase activity was detected and characterized in the fat body of  Triatoma brasiliensis one of the vector of Chagas' disease. The enzyme activity was close to a free fatty acid production of 47 mumol FFA mg protein-1-h-1 when assayed in the complete system. 2. The enzyme presented maximum activity at pH 8.5. It appeared to be activated by heparin (2 U/ml) and to require a cofactor (rat serum lipoproteins) when assayed with simple triglyceride emulsions. The requirement for a FFA acceptor (albumin or CaCl2) was also observed. 3. Inhibition of enzyme activity by pyrophosphate, HDB, protamine sulphate and 1 m NaC1 indicated further that the enzyme from fat body of T. brasiliensis had the characteristic features of lipoprotein lipase from other sources.
mesh_terms: D000273:Adipose Tissue / Q000187:drug effects / Q000201:enzymology*; D000418:Albumins / Q000494:pharmacology; D000818:Animals; D002914:Chylomicrons / Q000378:metabolism; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D006493:Heparin / Q000494:pharmacology; D066298:In Vitro Techniques; D008071:Lipoprotein Lipase / Q000378:metabolism*; D009994:Osmolar Concentration; D012965:Sodium Chloride / Q000494:pharmacology; D014224:Triatoma / Q000201:enzymology*; D014225:Triatominae / Q000201:enzymology*; D014280:Triglycerides / Q000378:metabolism
chemical_list: D000418:Albumins; D002914:Chylomicrons; D005230:Fatty Acids, Nonesterified; D014280:Triglycerides; D012965:Sodium Chloride; D006493:Heparin; D008071:Lipoprotein Lipase