Glutathione peroxidase in dried blood spots.
A new procedure utilizing dried blood spots was developed for detecting glutathione peroxidase deficiency. Samples from a known patient with a partial defect and from rats with an induced deficiency were distinguished from respective control groups by their longer defluorescence endpoints. Samples from 100 patients with anemia and 2 phenyl-ketonuric infants on low-protein diets contained glutathione peroxidase activity similar to that in 82 controls, when screened for the enzyme defect by the new procedure.
D000328:Adult; D000740:Anemia / Q000201:enzymology; D000818:Animals; D002648:Child; D005453:Fluorescence; D005979:Glutathione Peroxidase / Q000097:blood* / Q000172:deficiency; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008403:Mass Screening; D008661:Metabolism, Inborn Errors / Q000201:enzymology; D008722:Methods; D008875:Middle Aged; D009249:NADP / Q000378:metabolism; D010544:Peroxidases / Q000097:blood*; D010661:Phenylketonurias / Q000201:enzymology; D051381:Rats; D012643:Selenium / Q000172:deficiency
D009249:NADP; D010544:Peroxidases; D005979:Glutathione Peroxidase; D012643:Selenium