Effect of viomycin on dihydrostreptomycin binding to bacterial ribosomes.
Viomycin, a peptide antibiotic, reduced the amounts of dihydrostreptomycin bound to ribosomes of Myobacterium smegmatis and Escherichia coli, although they have different modes of action. The [3H]dihydrostreptomycin binding to ribosomes could not exchanged with streptomycin or dihydrostreptomycin, but not with unrelated antibiotics, namely, kanamycin, neomycin, spectinomycin, capreomycin, tuberactinomycin-N, chloramphenicol and erythromycin. We suggest that there is a significant interaction between the binding sites of viomycin and streptomycin on ribosomes.
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001665:Binding Sites; D001667:Binding, Competitive; D004096:Dihydrostreptomycin Sulfate / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D007700:Kinetics; D009161:Mycobacterium / Q000187:drug effects; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism*; D014756:Viomycin / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D004096:Dihydrostreptomycin Sulfate; D014756:Viomycin