[Lysosome-like alpha-granules of thrombocytes: detection by acridine orange treatment, properties and transformations during the course of blood coagulation reactions].
Using the ability of lysosome-like alpha-granules of thrombocytes to accumulate flurochrome acridine orange, their transformation kinetics was studied in the course of cell viscous metamorphosis utilizing the fluorometric technique. It turned out that during the thrombin induced cellular viscous metamorphosis lysosomes released their contents: the process is stimulated by the presence of extracellular Ca2+. Competitive relations were revealed between the accumulation within cytoplasmic granules of thrombocytes of acridine orange and serotonin, and that of histamine and novocaine. The process of fluorochrome accumulation was found to be temperature dependent and to be inhibited by monoiodacetate.
D000166:Acridines; D000818:Animals; D001777:Blood Coagulation; D001792:Blood Platelets / Q000166:cytology* / Q000187:drug effects; D002118:Calcium / Q000494:pharmacology; D003594:Cytoplasmic Granules; D006632:Histamine / Q000494:pharmacology; D007461:Iodoacetates / Q000494:pharmacology; D008247:Lysosomes; D011343:Procaine / Q000494:pharmacology; D011817:Rabbits; D012701:Serotonin / Q000494:pharmacology; D013194:Staining and Labeling
D000166:Acridines; D007461:Iodoacetates; D012701:Serotonin; D011343:Procaine; D006632:Histamine; D002118:Calcium
Lizosomopodobnye al'fa-granuly trombotsitov: vyiavlenie pri obrabotke akridinovym oranzhevym, nekotorye svoĭstva i preobrazovaniia v khode reakstii gemokoaguliastsii