Novel mannitol-containing oligosaccharides obtained by mild alkaline borohydride treatment of a chondroitin sulfate proteoglycan from brain.
Mannitol-containing oligosaccharides have been isolated from a rat brain proteoglycan after mild alkaline borohydride treatment under conditions which prevent "peeling." Their structural properties were studied by gas-liquid chromatography-mass spectrometry of disaccharides as their trimethylsilylated and permethylated derivatives, methylation, analysis, specific degradations, and CrO3 oxidation. The following components were identified: Gal(beta 1 leads to 4) [Fuc(alpha 1 leads to 3)]GlcNAc(beta 1 leads to 3)Manol,GlcNAc(beta 1 leads to 3)Manol, and Manol. Evidence was also obtained for the occurrence of a sialylated oligosaccharide and another (possibly sulfated) acidic oligosaccharide, both having the sequence GlcNAc(beta 1 leads to 3)Manol at their proximal ends. These mannitol-containing oligosaccharides constitute a novel group of alkali-labile oligosaccharides in mammalian glycoconjugates. The origin of the oligosaccharides and the possible occurrence of a carbohydrate-peptide linkage involving mannose are discussed.
D000818:Animals; D001894:Borohydrides; D001923:Brain Chemistry; D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000032:analysis; D002807:Chondroitin / Q000031:analogs & derivatives*; D002809:Chondroitin Sulfates / Q000032:analysis*; D002849:Chromatography, Gas; D006863:Hydrogen-Ion Concentration; D008353:Mannitol / Q000032:analysis*; D013058:Mass Spectrometry; D009844:Oligosaccharides / Q000032:analysis*; D011509:Proteoglycans / Q000302:isolation & purification; D051381:Rats
D001894:Borohydrides; D002241:Carbohydrates; D009844:Oligosaccharides; D011509:Proteoglycans; D008353:Mannitol; D002807:Chondroitin; D002809:Chondroitin Sulfates