The use of a transesterification technique to distinguish between certain neuraminidase resistant epithelial mucins.
The effects of potassium hydroxide and soidum methoxide treatments upon the Alcian blue staining and neuraminidase lability of certain neuraminidase resistant epithelial mucins have been studied. The results were interpreted as indicating that while the mucins of rat colon and rabbit Brunner's gland contain only 4-0-acetyl sialic acid, human colonic epithelial mucins may contain some sialic acid with esters at the C1 carboxyl group.
D000818:Animals; D004951:Esterification; D006025:Glycosaminoglycans / Q000032:analysis*; D006652:Histological Techniques; D006801:Humans; D006878:Hydroxides; D007413:Intestinal Mucosa / Q000032:analysis*; D008722:Methods; D009439:Neuraminidase; D011188:Potassium; D011817:Rabbits; D051381:Rats; D012794:Sialic Acids / Q000032:analysis*; D013194:Staining and Labeling
D006025:Glycosaminoglycans; D006878:Hydroxides; D012794:Sialic Acids; D009439:Neuraminidase; D011188:Potassium