The fate of fetal and adult B-cell progenitors grafted into immunodeficient CBA/N mice.
The relative ability of various precursors to generate functional B cells in vivo was assessed by transferring normal, chromosomally-marked CBA/H-T6T6 cells to irradiated or unirradiated immunodeficient CBA/N mice. Emergence of donor-derived B cells was monitored by means of a B-cell cloning assay (in which CBA/N cells are inactive), and by karyotpic analysis of lymphoid, myeloid, and stem cell metaphases. Grafts of lymph node, spleen, anti-mu surface immunoglobin suppressed bone marrow, sIg+ cell-depleted marrow, normal marrow, fetal liver, and yolk sac suggest: (a) there is little self-renewal of sIg+ B cells in these models; (b) pre-committed cells have extensive proliferative/differentiative potential and at least initially contribute most of the newly-formed B cells; (c) populations or pre-B cells obtained from various sources differ in their regenerative ability; (d) CBA/N mice are deficient in a category of pre-B cells which are found in fetal liver; and (e) selective B-cell chimerism results from grafting of unirradiated CBA/N mice.
D000367:Age Factors; D000818:Animals; D001402:B-Lymphocytes / Q000637:transplantation*; D002454:Cell Differentiation; D002455:Cell Division; D005260:Female; D005333:Fetus / Q000166:cytology; D018380:Hematopoietic Stem Cell Transplantation; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D011247:Pregnancy; D011828:Radiation Chimera; D011947:Receptors, Antigen, B-Cell; D014184:Transplantation, Homologous
D011947:Receptors, Antigen, B-Cell