A mouse hepatoma cell line which secretes several serum proteins including albumin and alpha-foetoprotein.
A permanent cell line (BW) was established from a transplantable mouse hepatoma, BW7756, which produces alpha-foetoprotein (AFP). Three clones were isolated from the uncloned culture: BW1, BW2 and BWTG3. The cells of the latter clone, which was isolated after selection in the presence of thioguanine, are deficient in the enzyme hypoxanthine-guanine-phosphoribosyl transferase. Both BW1 and BWTG3 cells have mean chromosome number of 64 (60 telocentric and 4 metacentric chromosomes). All three clones secrete at least five serum proteins into the culture medium: albumin, AFP, and alpha 2 globulin, transferrin and C3, the third component of complement. The approximate rate of albumin secretion by BW1 and BWTG3 cells is 10 mug/24 h/10(6) cells. Both albumin and AFP can easily be detected in cell extracts. The simultaneous production of AFP and a hepatocyte specific marker (albumin) by cloned hepatoma cells show that the production of AFP by the tumour is due to the tumoural hepatocytes themselves.
D000510:Alpha-Globulins / Q000378:metabolism; D000818:Animals; D001798:Blood Proteins / Q000096:biosynthesis / Q000378:metabolism*; D006528:Carcinoma, Hepatocellular; D002460:Cell Line; D002875:Chromosomes; D002999:Clone Cells / Q000378:metabolism; D003176:Complement C3 / Q000096:biosynthesis / Q000378:metabolism; D005326:Fetal Proteins / Q000378:metabolism*; D007041:Hypoxanthine Phosphoribosyltransferase; D007621:Karyotyping; D008113:Liver Neoplasms; D051379:Mice; D009154:Mutation; D012709:Serum Albumin / Q000096:biosynthesis / Q000378:metabolism*; D014168:Transferrin / Q000096:biosynthesis / Q000378:metabolism; D000509:alpha-Fetoproteins / Q000096:biosynthesis / Q000378:metabolism*
D000510:Alpha-Globulins; D001798:Blood Proteins; D003176:Complement C3; D005326:Fetal Proteins; D012709:Serum Albumin; D014168:Transferrin; D000509:alpha-Fetoproteins; D007041:Hypoxanthine Phosphoribosyltransferase