The effect of sulfasalazine and its active components on human polymorphonuclear leukocyte function in relation to ulcerative colitis.
Sulfasalazine and its active components, 5-aminosalicylic acid (5-ASA) and sulfapyridine (SP), are potent modulators of inflammatory reactions but with somewhat different modes of action. Investigating the effect of these compounds on normal human polymorphonuclear leukocytes in vitro, we show inhibition of different stages in the phagocytic process, such as migration (sulfasalazine and SP), superoxide production (sulfasalazine and SP), myeloperoxidase-mediated iodination and cytotoxicity (5-ASA and SP). It is thus suggested that sulfasalazine is not just a vehicle for delivering its active components in the colon, but that its therapeutic effect is ulcerative colitis and other inflammatory reactions is a result of the concurrent action of the three compounds.
D000293:Adolescent; D000328:Adult; D000636:Aminosalicylic Acids / Q000494:pharmacology; D002465:Cell Movement / Q000187:drug effects; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D003093:Colitis, Ulcerative / Q000188:drug therapy* / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D005347:Fibroblasts / Q000276:immunology; D006801:Humans; D007111:Immunity, Cellular / Q000187:drug effects; D008247:Lysosomes / Q000201:enzymology; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism; D009195:Peroxidase / Q000494:pharmacology; D010587:Phagocytosis / Q000187:drug effects; D013427:Sulfapyridine / Q000494:pharmacology; D012460:Sulfasalazine / Q000627:therapeutic use*
D000636:Aminosalicylic Acids; D012460:Sulfasalazine; D009195:Peroxidase; D013427:Sulfapyridine