[Bidimensional immunoelectrophoretic study of the antigenic composition of the membrane in various mycoplasma strains].
Membrane antigenic composition of Acheloplasma laidlawii PG9, A. granularum BTS-39, and Mycoplasma fermentans PG 18(G) was determined by means of bidimensional immunoelectrophoresis in the presence of sodium desoxycholate 0.5%. Depending upon the mycoplasma species from which membranes were obtained, 7 to 15 antigens were evidenced. Using sodium desoxycholate presents the advantage over non-ionic detergents to dissolve better the mycoplasmic membrane antigenic complexes. A comparative study of five strains belonging to the above-noted species confirms the serological heterogeneity of the Mycoplasmateles order and shows variability at the membrane antigenic composition level of Acheloplasma laidlawii.
D000127:Acholeplasma / Q000276:immunology*; D000942:Antigens, Bacterial / Q000032:analysis*; D002462:Cell Membrane / Q000276:immunology; D003840:Deoxycholic Acid; D000939:Epitopes; D007123:Immunoelectrophoresis, Two-Dimensional; D009174:Mycoplasma / Q000276:immunology*
D000942:Antigens, Bacterial; D000939:Epitopes; D003840:Deoxycholic Acid
Etude par immunoélectrophorèse bidimensionnelle de la composition antigénique de la membrane de quelques souches de mycoplasms