A study of DNA depolymerisation during Feulgen acid hydrolysis.
The binding of Schiff dye molecules after acid hydrolysis (1 M HCl) for varying lengths of time was studied in ascites tumour cells. The amount of dye bound to the tumour cells closely followed the number of aldehyde groups, calculated from the extraction of radioactive nucleotides. This constant dye to aldehyde ratio did not change when the hydrolysis was performed at a lower acid concentration (0.3 M HCl). The conclusion drawn is that Feulgen dye measurements represent, in a constant way, the number of aldehydes on DNA at any given time during hydrolysis. The alteration of the hydrolysis pattern of chromatin fixed in formalin was found to be due to a slower extraction of DNA depolymerisation products, the purine liberation being unaffected. A similar explanation is offered for the extreme pattern obtained from hydrolysis of bull spermatozoa chromatin.
D000225:Adenine / Q000032:analysis; D000447:Aldehydes / Q000032:analysis; D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000032:analysis; D002843:Chromatin / Q000378:metabolism; D004273:DNA, Neoplasm / Q000032:analysis; D005470:Fluorometry; D006651:Histocytochemistry; D006851:Hydrochloric Acid; D006868:Hydrolysis; D008297:Male; D051379:Mice; D011485:Protein Binding; D012545:Schiff Bases; D013194:Staining and Labeling; D013936:Thymidine / Q000032:analysis; D014316:Tritium
D000447:Aldehydes; D002843:Chromatin; D004273:DNA, Neoplasm; D012545:Schiff Bases; D014316:Tritium; D000225:Adenine; D006851:Hydrochloric Acid; D013936:Thymidine