Age-related mitochondrial lipoperoxidation in human skin.
Mitochondria were isolated from the epidermis of human subjects of different ages by a procedure that included pretreatment of the tissue with bacterial protease before homogenization. That epidermal mitochondria catalyze enzymatic lipid peroxidation in the presence of NADPH and chelated iron (ADP-Fe3+) is indicated by the production of malonaldehyde. The process does not appear to be influenced by age, and it is strongly enhanced in preparations containing a small amount of melanin.
D000293:Adolescent; D000367:Age Factors; D000368:Aged; D000375:Aging; D002648:Child; D006801:Humans; D066298:In Vitro Techniques; D007502:Iron Chelating Agents; D050356:Lipid Metabolism; D008875:Middle Aged; D008928:Mitochondria / Q000378:metabolism*; D009249:NADP; D010545:Peroxides / Q000378:metabolism; D012867:Skin / Q000378:metabolism*
D007502:Iron Chelating Agents; D010545:Peroxides; D009249:NADP