[Zinc-iodine-osmium impregnation of giant synaptosomes obtained from the hippocampal formation of the rabbit].
The fraction of giant synaptosomes from the r. inferior of the rabbit hippocampus was studied using impregnation with zinc iodide-osmium tetroxide (XIO) reagent and electron microscopy. In this fraction, light and dark synaptosomes were observed. The reaction product was found in the clear-centered synaptic vesicles (200-400 A) as electron-dense structures of different forms and small osmiophilic particles on the vesicular membranes. Dense-cored vesicles and postsynaptic structures were not revealed with ZIO-reagent. The structures revealed with ZIO-reagent in the giant synaptosomes of the hippocampus are supposedly related to stroage of the neurotransmitter-glutamate.
D000818:Animals; D006624:Hippocampus / Q000648:ultrastructure*; D007455:Iodine; D009992:Osmium; D011817:Rabbits; D013194:Staining and Labeling; D013570:Synaptic Membranes / Q000648:ultrastructure; D013572:Synaptic Vesicles / Q000648:ultrastructure; D013574:Synaptosomes / Q000648:ultrastructure*; D015032:Zinc
D009992:Osmium; D007455:Iodine; D015032:Zinc
Tsink-iod-osmievaia impregnatsiia gigantskikh sinaptosom, vydelennykh iz gippokampovoi formatsii krolika