title: Bunyavirus development in arctic and Aedes aegypti mosquitoes as revealed by glucose oxidase staining and immunofluorescence.
abstract: Northway virus replication has been detected in salivary glands of wild-caught Culiseta inornata and Aedes communis mosquitoes from the western Canadian Arctic after incubation at 4 degrees C for 9 to 11 months, and after incubation at 13 degrees C for 3 to 4 months after they received virus by oral ingestion or intrathoracic injection. Aedes hexodontus supported Northway virus replication after incubation at 13 degrees C for one month after intrathoracic injection. Aedes aegypti supported Northway virus replication after incubation at 13 degrees C or 23 degrees C for 6 to 28 days following intrathoracic injection. A larval isolate of California encephalitis virus (snowshoe hare subtype) multiplied in all 3 species of arctic mosquito after incubation at 13 degrees C for 1 to 3 months after virus was administered by oral ingestion or intrathoracic injection. Virus was detected in salivary glands of Cs. inornata after 329 days incubation at 4 degrees C after intrathoracic injection. Bunyavirus antigens in salivary glands of arctic and domestic mosquitoes were detected by the glucose oxidase immunoenzyme technique somewhat less frequently than by assay for virus infectivity.
mesh_terms: D000330:Aedes / Q000382:microbiology*; D000818:Animals; D001103:Arboviruses / Q000254:growth & development*; D001110:Arctic Regions; D002042:Bunyamwera virus / Q000254:growth & development*; D009033:Culicidae / Q000382:microbiology*; D002141:Encephalitis Virus, California / Q000254:growth & development*; D004668:Encephalitis Viruses / Q000254:growth & development*; D005260:Female; D005455:Fluorescent Antibody Technique; D005949:Glucose Oxidase; D007124:Immunoenzyme Techniques; D012469:Salivary Glands / Q000382:microbiology; D013696:Temperature; D014779:Virus Replication
chemical_list: D005949:Glucose Oxidase