title: Selective heat precipitability or reduced human hemoglobins in alkaline urea.
abstract: Solutions of reduced hemoglobins from patients with either SS, SC, or CC hemoglobinopathies precipitated when heated for two minutes at 100 degrees C at pH 9.20 in the presence of 3.64 M urea, while hemolysates of normal adult AA and newborns (FA hemoglobin) remained clear. In patients with hemoglobin C trait (AC) having 30% or more C hemoglobin, precipitation also occurred. The conditions of pH and urea concentration affording precipitation of hemoglobin S approximated those observed for hemoglobin C.
mesh_terms: D011232:Chemical Precipitation; D006441:Hemoglobin A / Q000032:analysis; D006444:Hemoglobin C / Q000032:analysis*; D006451:Hemoglobin, Sickle / Q000032:analysis*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010084:Oxidation-Reduction; D014508:Urea
chemical_list: D006451:Hemoglobin, Sickle; D014508:Urea; D006444:Hemoglobin C; D006441:Hemoglobin A