[Demonstration of immunoglobulin determinants on lymphocyte surfaces by membrane immunofluorescence].
Isolated lymphocytes from peripheral blood of 22 healthy donors were examined with the help of the indirect membrane immunofluorescence (MIF) for immunoglobulin determinants on the surface of the cells. The quantity of MIF+-cells for IgM was on the average 8.7% (3-18) and for IgG 15.5%(8-18). Five different anti-IgM-sera were characterised with regard to their use in the MIF (precipitation titre, entire protein content, content of anti-IgM-antibodies). Differences in the quantity of the MIF+-lymphocytes were above all based on differing antibody concentrations of the sera. It was referred to further possible causes for these differences. The influence on the results of the examinations by protein addition and trypsination.
D000328:Adult; D002462:Cell Membrane / Q000276:immunology; D000939:Epitopes; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007106:Immune Sera; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D008214:Lymphocytes / Q000276:immunology*; D008858:Microscopy, Phase-Contrast; D011947:Receptors, Antigen, B-Cell / Q000032:analysis*; D013154:Spleen / Q000166:cytology
D000939:Epitopes; D007106:Immune Sera; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011947:Receptors, Antigen, B-Cell
Zur Darstellung von Immunglobulindeterminanten an der Lymphozytenoberfläche mit Hilfe der Membranimmunfluoreszenz