Production of L-serine by Sarcina albida.
Conditions for the production of microbial L-serine hydroxymethyltransferase and for the conversion of glycine to L-serine were studied. A number of microorganisms were screened for their abilities to form and accululate L-serine from glycine, and Sarcina albida was selected as the best organism. Enzyme activity in this organism as high as 0.12 U/ml could be produced in shaken cultures at 30 degrees C in a medium containing glucose, ammonium sulfate, glycine, yeast extract, and inorganic salts. L-Serine was produced most efficiently by shaking cells at 30 degrees C in a reaction mixture containing 20% glycine, 5 X 10(-3) M formaldehyde, and 3 X 10(-4) M pyridoxal phosphate in yields of 22 mg of broth in 5 days. L-Serine was easily isolated in 84% yields by ion-exchange resin.
D001419:Bacteria / Q000378:metabolism; D003470:Culture Media; D005998:Glycine / Q000378:metabolism; D012696:Glycine Hydroxymethyltransferase / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D012695:L-Serine Dehydratase / Q000378:metabolism; D012505:Sarcina / Q000201:enzymology / Q000378:metabolism*; D012694:Serine / Q000096:biosynthesis*; D013045:Species Specificity; D013237:Stereoisomerism; D013696:Temperature
D003470:Culture Media; D012694:Serine; D012696:Glycine Hydroxymethyltransferase; D012695:L-Serine Dehydratase; D005998:Glycine