Interaction of C-reactive protein with lymphocytes and monocytes: complement-dependent adherence and phagocytosis.
The serum constituent C-reactive protein (CRP), which activates the classical complement (C) pathway when reacting with its substrates, was examined for its ability to mediate reactions of opsonic adherence and phagocytosis. Erythrocytes coated with C-polysaccharide (CPS) and reacted with CRP (E. CPS-CRP) failed to adhere to B cells and displayed only minimal adherence to monocytes. However, upon the addition of absorbed C or purified C components these cells were found to possess the cleavage products C4b and C3b, which in turn resulted in attachment of these cells to both human B lymphocytes and peripheral blood monocytes. E. CPS-CRP treated with C in the absence of antibody were readily phagocytosized by glass-adherent human monocytes. The phagocytosis of E. CPS-CRP-C was not only mediated by CRP but also required the presence of CRP on the surface of the red cells. The extent of ingestion was proportional to the amount of CRP on the red cell intermediate and was reduced by blocking monocyte receptors with aggregated human gamma-globulin (HGG) at concentrations which did not impair the uptake of other particles. The mediation by CRP of reactions of opsonic adherence and phagocytosis as outlined in these studies points to a significant role for CRP in reactions of host defense and inflammation.
D002097:C-Reactive Protein / Q000502:physiology*; D003176:Complement C3; D003165:Complement System Proteins / Q000502:physiology*; D006801:Humans; D007104:Immune Adherence Reaction; D007141:Immunoglobulin Fc Fragments; D008214:Lymphocytes / Q000276:immunology*; D009000:Monocytes / Q000276:immunology*; D009895:Opsonin Proteins; D010587:Phagocytosis / Q000187:drug effects; D011955:Receptors, Drug; D005719:gamma-Globulins / Q000494:pharmacology
D003176:Complement C3; D007141:Immunoglobulin Fc Fragments; D009895:Opsonin Proteins; D011955:Receptors, Drug; D005719:gamma-Globulins; D003165:Complement System Proteins; D002097:C-Reactive Protein