Hepatitis B core antigen. Detection of antibody by radioimmunoprecipitation.
Radioactive cores were prepared from concentrated Dane particles by DNA polymerase reaction followed by CsCl density gradient centrifugation. Two radioactive peaks were obtained: one peak with an average density of 1.36 g/cm-3 in CsCl contained cores that possessed full serologic reactivity; the other peak, with a density of 1.28 to 1.32 g/cm-3, contained cores associated with globulin. A double antibody immunoprecipitation test was developed, using the radioactive heavy cores as a source of antigen. The test was at least 300 times as sensitive as complement fixation for detecting antibody to core.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000914:Antibodies, Viral / Q000032:analysis*; D002353:Carrier State; D002499:Centrifugation, Density Gradient; D003168:Complement Fixation Tests; D004254:DNA Nucleotidyltransferases / Q000378:metabolism; D000939:Epitopes; D004912:Erythrocytes / Q000276:immunology; D006041:Goats / Q000276:immunology; D006386:Hemagglutination Tests; D006509:Hepatitis B / Q000276:immunology*; D006510:Hepatitis B Antibodies / Q000032:analysis*; D006511:Hepatitis B Antigens; D006801:Humans; D007106:Immune Sera; D002679:Pan troglodytes; D011233:Precipitin Tests; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay; D013634:Tannins; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D000906:Antibodies; D000914:Antibodies, Viral; D000939:Epitopes; D006510:Hepatitis B Antibodies; D006511:Hepatitis B Antigens; D007106:Immune Sera; D013634:Tannins; D014316:Tritium; D004254:DNA Nucleotidyltransferases; D013936:Thymidine