Phosphate uptake in chemostat cultures of Escherichia coli K-12 subjected to periodic beta-glycerophosphate pulsing: a system for assaying alkaline phosphatase.
Limiting concentrations of beta-glycerophosphate were pulsed into chemostat cultures of Escherichia coli K-12 at intervals equal to the population doubling time. The resultant culture density fluctuations are interpreted in terms of inorganic phosphate uptake which, in this system, is a function of alkaline phosphatase activity. Information concerning in vivo alkaline phosphatase activity at suboptimal (acidic) pH with very low concentrations of substrate (beta-glycerophosphate) is obtained from kinetic analysis of uptake data.
D000469:Alkaline Phosphatase / Q000378:metabolism*; D003470:Culture Media; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism*; D005994:Glycerophosphates / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D010507:Periodicity; D010710:Phosphates / Q000378:metabolism*
D003470:Culture Media; D005994:Glycerophosphates; D010710:Phosphates; D000469:Alkaline Phosphatase