Selective light microscopic demonstration of the specific granulation of the rat atrial myocardium by lead-hematoxylin-tartrazine.
MacConaill's lead-hematoxylin as modified by Solcia et al. was found to be a highly selective stain for the specific granulation of atrial cardiocytes in the rat. The specific atrial granules were stained blue-black. Contrast was enhanced by counterstaining in a saturated solution of tartrazine in Cellosolve. The stain is compatible with several fixatives and may be used with paraffin or Epon-embedded material.
D000818:Animals; D001391:Azo Compounds; D001557:Benzenesulfonates; D002466:Cell Nucleolus / Q000648:ultrastructure; D002467:Cell Nucleus / Q000648:ultrastructure; D003594:Cytoplasmic Granules / Q000648:ultrastructure*; D006325:Heart Atria / Q000648:ultrastructure*; D006352:Heart Ventricles / Q000648:ultrastructure; D006416:Hematoxylin; D007854:Lead; D051381:Rats; D012519:Sarcoplasmic Reticulum / Q000648:ultrastructure; D013194:Staining and Labeling / Q000379:methods
D001391:Azo Compounds; D001557:Benzenesulfonates; D007854:Lead; D006416:Hematoxylin