title: Incorporation of radioactive precursors into filarial larvae of Brugia developing in susceptible and refractory mosquitoes.
abstract: The incorporation of tritiated precursors injected into mosquito hosts parasitized by developing filarial larvae of Brugia patei has been studied by autoradiography in 2 species of mosquito, Aedes togoi in which filarial development was normal and Anopheles labranchiae atroparvus in which filarial development was abnormal. In both mosquito hosts there was significant incorporation into 4--5-day-old developing larvae of uridine and amino acids (isoleucine, leucine, valine, arginine, lysine, cystine, methionine, phenylalanine, tyrosine, tryptophan, histidine, and proline), although lower incorporation of methionine, tyrosine, and tryptophan was found during abnormal development. No incorporation of thymidine, hydroxytryptophan, dopa, or carbohydrate was found at this stage of larval development. Some incorporation of glucose and dopa was found in or around earlier stages of development in An. l. atroparvus. Mosquito flight muscle showed lower incorporation of glucose, but not of amino acids, around the site of filarial parasite development. The flight muscle of An. l. atroparvus showed a higher level of incorporation of lysine compared to that in A. togoi and higher levels of lysine and valine were found in the abnormally developing filarial larvae in the refractory mosquito.
mesh_terms: D000330:Aedes / Q000469:parasitology; D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D000852:Anopheles / Q000469:parasitology; D002009:Brugia / Q000254:growth & development / Q000378:metabolism*; D050260:Carbohydrate Metabolism; D009033:Culicidae / Q000378:metabolism / Q000469:parasitology*; D005370:Filarioidea / Q000378:metabolism*; D007814:Larva / Q000378:metabolism; D009132:Muscles / Q000378:metabolism; D009705:Nucleosides / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D009705:Nucleosides