Effect of incubation temperature on infectivity titration of mouse brain-passaged avian infectious bronchitis virus in laboratory host systems.
Mouse brain-passaged infectious bronchitis virus (IBV) could not be assayed for its infectivity at 39.5 degrees C, but could be so at 37 degrees C and 39.5 degrees C. Antigen accumulation was not detected by immunofluorescence in CEK cells infected with mouse brain-passaged IBV at 39.5 degrees C, perhaps due to the difference in the cellular environments.
D000818:Animals; D000956:Antigens, Viral; D001921:Brain / Q000382:microbiology; D002478:Cells, Cultured; D002642:Chick Embryo; D003332:Coronaviridae / Q000254:growth & development*; D001351:Infectious bronchitis virus / Q000254:growth & development* / Q000276:immunology; D007668:Kidney; D051379:Mice; D013696:Temperature; D014132:Trachea
D000956:Antigens, Viral