[Value of histospectrophotometry for the study of glycoproteins, glycosaminoglycans, and anionic polyglycans after staining with pseudo-isocyanine].
Pseudo-isocyanine, a basic stain, shows a characteristic metachromasia when associated with biological polyanions. The maximal absorption peak is very narrow and its wavelength is specific for the substances tested, in vitro as well as in tissue sections.
D000818:Animals; D002356:Cartilage / Q000032:analysis; D002417:Cattle; D004396:Coloring Agents; D003315:Cornea / Q000032:analysis; D006224:Cricetinae; D005260:Female; D006023:Glycoproteins / Q000032:analysis*; D006025:Glycosaminoglycans / Q000032:analysis*; D006651:Histocytochemistry; D006801:Humans; D010057:Oviducts / Q000032:analysis; D000459:Phaeophyceae / Q000032:analysis; D010904:Pituitary Gland, Posterior / Q000032:analysis; D011134:Polysaccharides / Q000032:analysis*; D051381:Rats; D013053:Spectrophotometry; D013194:Staining and Labeling; D014319:Triturus; D014470:Umbilical Cord / Q000032:analysis; D014562:Caudata; D014599:Uterus / Q000032:analysis
D004396:Coloring Agents; D006023:Glycoproteins; D006025:Glycosaminoglycans; D011134:Polysaccharides
Intérêt de l'histospectrophotométrie pour l'étude des glycoprotéines, des glycosaminoglycannes et des polyglycannes anioniques après coloration à la pseudo-isocyanine