A modified procedure for the preparation of UDP-beta-L-(U-14C)rhamnose.
This paper describes the synthesis of UDP-L-(U-14C)rhamnose from UDP-D-(U-14C)glucose and NADPH using an enzyme preparation of Nicotiana tabacum var. Xanthi. A procedure to separate UDP-l-rhamnose from the other compounds in the reaction mixture is described. Optimal separation was achieved in ethanol 95%-1 M ammonium acetate (pH 3.8) (7:3, v/v) at 30 degrees C.
D055598:Chemical Phenomena; D002621:Chemistry; D006863:Hydrogen-Ion Concentration; D010947:Plants, Toxic; D012210:Rhamnose / Q000031:analogs & derivatives* / Q000302:isolation & purification; D013237:Stereoisomerism; D013696:Temperature; D014026:Nicotiana / Q000201:enzymology*; D014539:Uridine Diphosphate Sugars
D014539:Uridine Diphosphate Sugars; D012210:Rhamnose