Protein synthesis in neural cells in culture: role of cell density and neurohumors.
Protein sythesis was studied in C-6 glial cells and neuroblastoma (NB) cells as a function  of cell density and after differentiation with dibutyryl cyclic AMP and treatment with either norepinephrine (NE), dopamine or L-dopa. In both C-6 glial cells and NB cells, unincorporated 3H-leucine decreased, whereas incorporation of 3H-leucine into protein increased with increasing cell density, particularly at high cell densities. Exposure of C-6 glial cells of NE at various dose for 60 minutes stimulated the efficiency of 3H-leucine corporation into protein. This effect was not seen with L-dopa or dopamine. In contrast to the glial cells, in neuroblastoma cells, all three neurohumors caused a decrease in the incorporation of 3H-leucine into protein. The increase in protein synthesis by NE was also seen in DBcAMP-differentiated glial cells. These findings suggest that cellular activity as reflected by protein synthesis is cell density dependent. In addition, neurohumor substances may play a regulatory role in the cellular activity of glial cells.
D003994:Bucladesine / Q000494:pharmacology; D002452:Cell Count; D002454:Cell Differentiation; D002460:Cell Line; D007980:Levodopa / Q000494:pharmacology; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D018377:Neurotransmitter Agents / Q000494:pharmacology*; D009638:Norepinephrine / Q000494:pharmacology; D014176:Protein Biosynthesis
D018377:Neurotransmitter Agents; D007980:Levodopa; D003994:Bucladesine; D009638:Norepinephrine