[Isolation and properties of preparations the branching enzyme of muscles, amylose isomerase].
The paper describes a modified method of isolating the branching enzyme of amylose isomerase from muscles and a study of the enzyme activity at different stages of purification. By enzyme fractionation on biogel R-150 and Sepharose 6B the fractions containing different RNA amounts have been isolated. The activity of fractions has been shown to depend on their content of RNA. The paper presents a procedure used to isolate a highly purified fraction of amylose isomerase and its properties (pH and temperature optima, enzyme optimal concentration and Michaelis constant).
D015061:1,4-alpha-Glucan Branching Enzyme / Q000302:isolation & purification*; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002384:Catalysis; D055598:Chemical Phenomena; D002621:Chemistry; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005964:Glucosyltransferases / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008722:Methods; D009132:Muscles / Q000201:enzymology*; D012313:RNA / Q000032:analysis; D011817:Rabbits; D012685:Sepharose; D013696:Temperature
D000596:Amino Acids; D012313:RNA; D012685:Sepharose; D005964:Glucosyltransferases; D015061:1,4-alpha-Glucan Branching Enzyme
Vydelenie i svoĭstva preparatov vetviashchego fermenta myshts izomerazy amilozy.