Microplate enzyme-linked immunosorbent assay for chagas' disease.
A microscale immunosorbent assay (E.L.I.S.A.) for the measurement of antibody in Chagas' disease (American trypanosomiasis) is described. The test was assessed on plasma samples from people living in Bahia, Brazil. 98% of the individuals with positive Trypanosoma cruzi immunofluorescent-antibody (I.F.A.) tests gave E.L.I.S.A. reading considered as positive. Less than 2% of those with negative I.F.A. tests had positive E.L.I.S.A. reactions. This simple test may be suitable for use in large seroepidemiological surveys.
D000327:Adsorption; D000469:Alkaline Phosphatase; D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D001800:Blood Specimen Collection; D001938:Brazil; D014355:Chagas Disease / Q000175:diagnosis / Q000276:immunology* / Q000382:microbiology; D005188:False Negative Reactions; D005189:False Positive Reactions; D005455:Fluorescent Antibody Technique; D006801:Humans; D007118:Immunoassay; D007074:Immunoglobulin G; D007136:Immunoglobulins; D011817:Rabbits / Q000276:immunology; D012698:Serologic Tests; D013053:Spectrophotometry; D014349:Trypanosoma cruzi / Q000276:immunology*
D000906:Antibodies; D007074:Immunoglobulin G; D007136:Immunoglobulins; D000469:Alkaline Phosphatase