Solubilization of human erythrocyte membrane glycoproteins by triton X-100.
1. The enzymic removal of sialic acid residues from the glycoproteins of the human erythrocyte decreases the solubilization of membrane glycoprotein by Triton X-100. 2. The solubilization of asialoglycoprotein by Triton X-100 may be restored by the addition of borate. 3. Use of this non-ionic detergent in the presence of borate, as a general procedure for the mild solubilization of membrane glycoproteins deficient in sialic acid residues, is discussed.
D001881:Borates; D004910:Erythrocyte Membrane / Q000032:analysis*; D004912:Erythrocytes / Q000032:analysis*; D006023:Glycoproteins / Q000097:blood*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D011092:Polyethylene Glycols
D001881:Borates; D006023:Glycoproteins; D011092:Polyethylene Glycols