[Highly permeable intercellular junctions in normal and transformed fibroblast cultures].
Intercellular junctions permeable to ions and fluorescein Na were studied with the aid of intracellular microelectrodes in the cultures of normal and transformed mouse-embryo and hamster-embryo fibroblast-like cells. Normal cells were effectively coupled by the highly permeable junctions. In cultures of 7 types of transformed cells, 3 types of coupling were detected: effective, decreased, and fully reduced couplings. The degree of uncoupling was not correlated with morphological patterns of malignization and tumorogeneity of transformed cultures. The decrease of permeability of intercellular junctions to ions and small molecules is concluded not to be necessary for malignization.
D000818:Animals; D002460:Cell Line; D002463:Cell Membrane Permeability; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D006224:Cricetinae; D003470:Culture Media; D003584:Cytological Techniques; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology*; D007365:Intercellular Junctions / Q000502:physiology*; D008564:Membrane Potentials; D051379:Mice; D008839:Microelectrodes; D008856:Microscopy, Fluorescence; D012513:Sarcoma, Experimental / Q000473:pathology; D013194:Staining and Labeling; D013997:Time Factors
D003470:Culture Media
Vysokopronitsaemye mezhkletochnye kontakty v kul'turakh normal'nykh itransformirovannykh fibroblastov