Metabolism of oestradiol by human mammary tumour 800 x g supernatants pretreated with dihydrolipoic acid.
Incubation of human mammary tumour 800 x g supernatants with [3H]oestradiol after preincubation for 10 min at 0 degrees C with 10 mM dihydrolipoic acid resulted in metabolism of oestradiol. This was noted in 12 malignant tumours, 8 of which contained measurable oestrogen receptor levels. In 2 benign tumours lacking measurable levels of receptor, dihydrolipoic acid pretreatment had no effect. This metabolism was further stimulated by pretreatment with NAD, NADP and the anti-oestrogen Tamoxifen (ICI 46,474). When incubations were carried out in an O2 atmosphere using oxygenated buffers, the effect was suppressed.
D001943:Breast Neoplasms / Q000378:metabolism*; D004958:Estradiol / Q000378:metabolism*; D006801:Humans; D008063:Thioctic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D014316:Tritium
D014316:Tritium; D004958:Estradiol; D008063:Thioctic Acid; C015144:dihydrolipoic acid