title: Removal of blood group determinants from bovine erythrocyte membranes. 2. Degradation of ghosts by butanol and pyridine.
abstract: Ghosts produced from erythrocytes collected from six different cattle were degraded with butanol and pyridine. Of a total of 38 different antigenic determinants available for investigation among the six cows, F, V, J and L were the only specificities detected in the subfractions resulting from either method of degradation. After butanol degradation V, J and L antigens were found in the soluble protein fraction, while F was found in the insoluble protein. Pyridine digestion resulted in all four determinants being detected in the sialoprotein layer, while J was found in the lipoprotein as well. All antigens were relatively weak, being detected in inhibition strengths of 10.0 to 1.25 mg/ml.
mesh_terms: D000818:Animals; D000440:Butanols / Q000494:pharmacology*; D002417:Cattle / Q000097:blood*; D002458:Cell Fractionation; D002462:Cell Membrane / Q000276:immunology; D000939:Epitopes; D004912:Erythrocytes / Q000276:immunology*; D006385:Hemagglutination Inhibition Tests; D011725:Pyridines / Q000494:pharmacology*
chemical_list: D000440:Butanols; D000939:Epitopes; D011725:Pyridines