title: [Investigation of adenosinetriphosphatase activity of rat liver and thymus cell nuclei].
abstract: The activity of ATPase was studied in highly purified rat liver and thymus cell nuclei, HCO3-, CO3(2-) and SO3(2-) stimulated nuclear ATPase in 1.5--2 times. HSO3- did not affect the enzyme activity, and NO3-, J-, ClO4-,F- and SCN- inhibited it. Bicarbonate increased V and decreased Ka for ATP. SCN- inhibited HCO3--ATPase activity non-competitively with respect to HCO3-. Mg2+-ATPase activity did not depend on pH, and HCO3-component of the activity was decreased under alkaline pH. Mg2+, Mn2+ and Co2+ increased the initial ATPase activity and helped its stimulation with HCO3-. Ba2+, Ni2+ and Zn2+ inhibited the ATPase activity, and Ca2+ did not affect it, Nuclear ATPase is sensitive to 2,4-dinitrophenol and DNAase. It is suggested that cell nuclei have their own H+-ATPase differing for some characteristics from mitochondrial H+-ATPase.
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D001639:Bicarbonates / Q000494:pharmacology; D002413:Cations, Divalent; D002467:Cell Nucleus / Q000201:enzymology*; D003851:Deoxyribonucleases / Q000378:metabolism; D004140:Dinitrophenols / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000201:enzymology*; D008274:Magnesium / Q000494:pharmacology; D051381:Rats; D013861:Thiocyanates / Q000494:pharmacology; D013950:Thymus Gland / Q000201:enzymology*
chemical_list: D001639:Bicarbonates; D002413:Cations, Divalent; D004140:Dinitrophenols; D013861:Thiocyanates; D003851:Deoxyribonucleases; D000251:Adenosine Triphosphatases; D008274:Magnesium
vernacular_title: Issledovanie adenozintrifosfataznoĭ aktivnosti iader kletok pecheni i timusa krysy.