Production of anaphylactic antibodies to drugs in experimental animals. I. Benzylpenicillin.
Studies were undertaken to determine if animals exposed to benzylpenicillin could produce anaphylactic antibodies to the drug moiety. For this purpose, Albino Wistar rats were divided into four groups. Two groups of animals were immunized with benzylpenicillin using either the intraperitoneal or the subcutaneous route. The remaining two groups were used for feeding experiments. The animals were bled upon termination of the experiments; their sera were collected and pooled per group. The pooled sera were tested for the presence of anaphylactic antibodies. Histamine release from rat mast cells under the appropriate conditions was used as an indicator of antibody production. Penicilloylpolylysine and benzylpenicillin were both employed to determine antibody specificity. It was found that sera from all groups of animals contained anaphylactic antibodies with specificity towards the penicilloyl group and "benzylpenicillin".
D000284:Administration, Oral; D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D000939:Epitopes; D005260:Female; D006168:Guinea Pigs; D006632:Histamine / Q000378:metabolism; D007114:Immunization; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007274:Injections, Intraperitoneal; D007279:Injections, Subcutaneous; D008297:Male; D008407:Mast Cells / Q000378:metabolism; D010400:Penicillin G / Q000008:administration & dosage / Q000276:immunology*; D051381:Rats
D000939:Epitopes; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D006632:Histamine; D010400:Penicillin G