[Study of bilirubin- albumin binding. I.-In vitro study by partition of bilirubin between an aqueous solution and an immiscible organic solvent (author's transl)].
The partition of bilirubin in vitro between water and isobutyl-methylketone/n-heptane mictures is investigated at different pH, in the absence and the presence of albumin. Results are used to compute the concentrations of unbound ionized and unionized bilirubin and the bilirubin-albumin characteristics (association constant K and nomber n of primary binding sites per mole of albumin). The effect of various factors upon these characteristics is discussed.
D000818:Animals; D001663:Bilirubin; D002417:Cattle; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008433:Mathematics; D012710:Serum Albumin, Bovine; D012995:Solubility; D012997:Solvents; D014867:Water
D012997:Solvents; D014867:Water; D012710:Serum Albumin, Bovine; D001663:Bilirubin
Etude de la liaison bilirubine-albumine. I-Etude in vitro par une méthode de partage de la bilirubine entre une solution aqueuse et un solvant organique non miscible à l'eau.