title: [Degradation of 14C, 3H, and 36Cl-labelled gamma-hexachlorocyclohexane by anaerobic soil microorganisms (author's transl)].
abstract: Up to 90% of the gamma-Hexachlorocyclohexane (gamma-HCH) applied to an anaerobic mixed bacterial flora enriched from an arable soil were degraded within 4-5 days. Degradation resulted in a rapid release of chloride and in formation of chlorine-free volatile metabolites. CO2 formation from the molecule was not detected. Investigations with 14C/3H- and 36Cl/3H double-labelled gamma-HCH indicated that the release of Cl and H did not occur in the ratio of 1:1. More Cl than H was split off. The volatile compounds contained more 14C than 3H. Gas chromatographic studies also showed the rapid decrease of gamma-HCH and the formation of several metabolities. gamma-Pentachlorocyclohexene was nto detected. Increasing O2-contents in the gas phase of cultures resulted in decreases of the compound's degradation. Release of chloride and of volatile metabolites were observed with O2 contents in the gas phase up to 5%. alpha-HCH was also, but more slowly as with gamma-HCH, degraded by the anaerobic mixed flora. Chloride was released and volatile, chlorine-free metabolites were found.
mesh_terms: D000693:Anaerobiosis; D001673:Biodegradation, Environmental; D002245:Carbon Dioxide / Q000378:metabolism; D002250:Carbon Radioisotopes; D002712:Chlorides / Q000378:metabolism; D002713:Chlorine; D002849:Chromatography, Gas; D001556:Hexachlorocyclohexane / Q000378:metabolism*; D006859:Hydrogen / Q000378:metabolism; D011868:Radioisotopes; D012988:Soil Microbiology; D014316:Tritium
chemical_list: D002250:Carbon Radioisotopes; D002712:Chlorides; D011868:Radioisotopes; D014316:Tritium; D002245:Carbon Dioxide; D002713:Chlorine; D001556:Hexachlorocyclohexane; D006859:Hydrogen
vernacular_title: Abbau von 14C-, 3H- und 36Cl-markiertem gamma-Hexachlorcyclohexan durch anaerobe Bodenmikroorganismen