Dissociation of the immunologic release of histamine and slow reacting substance of anaphylaxis from human lung using cytochalasins A and B-1.
Cytochalasins A and B at low doses resulted in enhancement of the antigen-induced and anti-IgE-mediated release of histamine from human lung fragments in vitro. Both compounds appeared to effect a dose-dependent inhibition of the antigen-induced release of slow reacting substance of anaphylaxis. Although the effects observed with cytochalasin B were at least partially reversible, the effects of cytochalasin A could not be readily reversed. The cytochalasins appeared to be more effective when present during the antigen-dependent activation phase of mediator release rather than during the calcium-dependent release phase.
D000818:Animals; D003571:Cytochalasin B / Q000494:pharmacology*; D003572:Cytochalasins / Q000494:pharmacology*; D006041:Goats / Q000276:immunology; D006636:Histamine Release / Q000187:drug effects*; D006801:Humans; D007106:Immune Sera; D007073:Immunoglobulin E; D008168:Lung / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D013189:SRS-A / Q000378:metabolism*
D003572:Cytochalasins; D007106:Immune Sera; D013189:SRS-A; D007073:Immunoglobulin E; D003571:Cytochalasin B