[Acid mucopolysaccharides in human blood and bone marrow cells shown by fluorescence microscopy (author's transl)].
In lymphocytes, monocytes and basophil granulocytes of patients with mucopolysaccharidosis specific fluorescence of acid mucopolysaccharides exists after fixation of blood smears with 9-aminoacridin-hydrochloride and subsequent staining with pseudoisocyanine at pH 4.2. A total of eleven children with various types of mucopolysaccharidosis were investigated. Specificaly stained granula were demonstrated in 5 to 49 percent of all lymphocytes and monocytes. Acid mucopolysaccharides were for the first time also shown in red-cell precursors of the bone marrow of children with mucopolysaccharidosis.
D000166:Acridines; D001773:Blood Cells / Q000032:analysis*; D001853:Bone Marrow / Q000032:analysis*; D001854:Bone Marrow Cells; D002648:Child; D006025:Glycosaminoglycans / Q000032:analysis* / Q000097:blood; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods; D009083:Mucopolysaccharidoses / Q000378:metabolism; D013194:Staining and Labeling
D000166:Acridines; D006025:Glycosaminoglycans
Fluoreszenzmikroskopischer Nachweis saurer Mucopolysaccharide in menschlichen Blut- und Knochenmarkzellen