Light dependence of osmium reactivity in mouse photoreceptor cells.
Mouse photoreceptor cells exhibit local accumulations of osmium deposits after prolonged osmic staining at slightly elevated temperatures. Deposits were evident along the membranes of outer segment lamellae, Golgi cisternae and vesicles, nuclear envelopes, and synaptic vesicles. Other membranes within the photoreceptor cells were unreactive. No osmium reactivity was seen in other cells of the retina except for osmiophilic outer segment material which had been phagocytized by the pigment epithelium. In the outer segments, inner segments, and synaptic regions of the photoreceptor cells, the amount of osmium reactivity was increased by light stimulation and decreased following extended dark adaptation. The possible significance of the localized osmium reactivity is discussed.
D000818:Animals; D002462:Cell Membrane / Q000648:ultrastructure; D003623:Dark Adaptation; D006056:Golgi Apparatus / Q000648:ultrastructure; D008027:Light; D051379:Mice; D008854:Microscopy, Electron; D009992:Osmium; D010587:Phagocytosis; D010786:Photoreceptor Cells / Q000648:ultrastructure; D012160:Retina / Q000648:ultrastructure; D013194:Staining and Labeling; D013572:Synaptic Vesicles / Q000648:ultrastructure
D009992:Osmium