Expression and segregation of HL-A antigens in D98/AH-2 by lymphocyte and fibroblast hybrids.
Somatic cell hybrids between D98/AH-2 and fibroblasts or peripheral blood lymphocytes were analysed for the expression of HL-A antigens. The hybrids express the antigens of the donor lymphocytes or fibroblasts as well as other HL-A specificities from D98/AH-2, a HeLa derivative on which no HL-A could be detected by direct cytotoxicity. Segregant hybrid clones could be used to determine HL-A haplotypes and define the linkage relationships between these haplotypes and the PGM3 alleles expressed in the hybrids. An analysis of the HL-A antigens on D98/AH-2 and other HeLa derivates was done using absorption and a modified cytotoxicity assay.
D000483:Alleles; D002460:Cell Line; D002999:Clone Cells; D000939:Epitopes; D005347:Fibroblasts / Q000276:immunology*; D008040:Genetic Linkage; D006680:HLA Antigens / Q000032:analysis*; D006238:Haploidy; D006649:Histocompatibility Antigens / Q000032:analysis*; D006801:Humans; D006822:Hybrid Cells / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology*
D000939:Epitopes; D006680:HLA Antigens; D006649:Histocompatibility Antigens