Use of chellators in the electron microscopic detection of mitochondrial adenosine triphosphatase.
Even when using modified WACHSTEIN-MEISEL procedure for the detection of mitochondrial ATP-ase it is difficult to avoid the non-specific reaction. Separation of the artefact from the precipitate of citric hydrolysis can be carried out only with difficulty. 20 mM of penicillin G amine or 40 mM of ascorbic acid in the incubation solution leads to selective localization. Ascorbic acid can also be used in the form of a 0.2 M tris and 0.05 M ascorbic acid buffer. With the use of chellators it is possible to eliminate factors which under pathological conditions greatly favour the formation of the artefact.
D000251:Adenosine Triphosphatases / Q000032:analysis*; D000818:Animals; D001205:Ascorbic Acid; D002614:Chelating Agents; D002951:Citrates; D004796:Clinical Enzyme Tests; D005260:Female; D006801:Humans; D007854:Lead; D008931:Mitochondria, Muscle / Q000201:enzymology* / Q000648:ultrastructure; D010406:Penicillins; D051381:Rats; D013194:Staining and Labeling
D002614:Chelating Agents; D002951:Citrates; D010406:Penicillins; D007854:Lead; D000251:Adenosine Triphosphatases; D001205:Ascorbic Acid