Lifetime of the purple intermediate of D-amino acid oxidase under anaerobic conditions.
The lifetime of the purple intermediate formed from D-amino acid oxidase [D-amino acid: O2 oxidoreductase (deaminating), EC 1.4.3.3] and a neutral D-amino acid under anaerobic conditions was measured with a series of neutral D-amino acids. The lifetime increases with increase in the number of carbons in the side chain of the amino acid up to 4-5 atoms and then decreases with further increase in the number of carbons. This suggests that the hydrophobicity of the alkyl group of the neutral D-amino acid determines the lifetime of the purple intermediate unless a steric effect occurs.
D000693:Anaerobiosis; D003605:D-Amino-Acid Oxidase / Q000378:metabolism*; D004355:Drug Stability; D007700:Kinetics; D010084:Oxidation-Reduction; D013053:Spectrophotometry
D003605:D-Amino-Acid Oxidase