Prostaglandin F2alpha and human prostatic affinity for testosterone.
Earlier work had shown that the lactogen, LTH and HPL, foster testosterone binding by the prostate. This study was undertaken to see if prostaglandin F2alpha would oppose the effect of the lactogen on the prostate as it does the luteotrophic action of the hormone on the corpus luteum. When it was found instead that the PGF increases steroid binding and that its interaction with lactogen was neither antagonistic nor additive, attention was directed to further characterization of prostaglandin's effect. A dosage/response study of F2alpha alone showed that concentrations of 4 ng/ml and 40 ng/ml increased binding but that 400 ng/ml did not. Glands with stromal hyperplasia and/or inflammation were not responsive than those with epithelial hyperplasia. Assays of water extracts of the tissue revealed concentrations of about 340 ng of F2alpha per gram fresh weight and that the concentration varied inversely as the beta-glucuronidase activity. If the enzyme level is considered an index of the epithelial cell density within the specimen, the inverse relationship suggest a non-epithelial (stromal) site of prostaglandin concentration.
D004305:Dose-Response Relationship, Drug; D005966:Glucuronidase / Q000378:metabolism; D006801:Humans; D008297:Male; D011453:Prostaglandins / Q000008:administration & dosage / Q000494:pharmacology*; D011467:Prostate / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D011470:Prostatic Hyperplasia / Q000378:metabolism; D013268:Stimulation, Chemical; D013739:Testosterone / Q000378:metabolism*
D011453:Prostaglandins; D013739:Testosterone; D005966:Glucuronidase