Serum-stimulated lipases (lipoprotein lipases). Immunological crossreaction between the bovine and the human enzymes.
A rabbit antiserum prepared against the serum-stimulated lipase (lipoprotein lipase) from bovine milk crossreacted with serum-stimulated lipases from human milk and from human postheparin plasma, but not with bile salt-stimulated lipase from human milk or with salt-resistant lipase from human postheparin plasma. Thus, the serum-stimulated lipase in bovine milk has immunological determinants in common with the serum-stimulated lipases in human milk and in human postheparin plasma. The time-courses for the appearance of serum-stimulated lipase and salt-resistant lipase activities in human plasma after heparin injection were different. The two activities were separated by heparin-Sepharose chromatography. After treatment of postheparin plasma with the antiserum only the salt-resistant lipase activity could be eluted from the column. Thus, these two enzyme activities in postheparin plasma reside in two different enzyme molecules.
D000818:Animals; D001769:Blood; D002417:Cattle; D003429:Cross Reactions; D005260:Female; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D008049:Lipase / Q000276:immunology* / Q000378:metabolism; D008071:Lipoprotein Lipase / Q000276:immunology* / Q000378:metabolism; D008892:Milk / Q000201:enzymology*; D008895:Milk, Human / Q000201:enzymology*; D011247:Pregnancy; D005719:gamma-Globulins
D005719:gamma-Globulins; D006493:Heparin; D008049:Lipase; D008071:Lipoprotein Lipase