Phosphonoglycoprotein from Metridium senile--heterogeneity of glycoproteins containing aminoethylphosphonic acid.
1. After separation by SDS gel-chromatography, analysis of AEP-containing glycoproteins from M. senile, indicated 66% amino acids with 220 AEP res./1000 res. and 30% carbohydrate for high mol. wt (greater than 10(7) forms and 80% amino acids with 25-50 AEP res./1000 res. and 10% carbohydrate for low mol. wt (2-4 x 10(4) forms. 2. Uronic acids, sulfate, lipid, and sialic acids were absent. 3. Mild base digestion released AEP-hexosamine containing oligosaccharides and destroyed ser-thr residues in the high mol. wt components. 4. Phosphonoglycoproteins appear to be acidic connective tissue components with AEP linked to hexosamine containing oligosaccharide side chains.
D000596:Amino Acids / Q000032:analysis; D000615:Aminoethylphosphonic Acid / Q000378:metabolism*; D000818:Animals; D002850:Chromatography, Gel; D003063:Cnidaria / Q000378:metabolism*; D006023:Glycoproteins / Q000302:isolation & purification / Q000378:metabolism*; D006868:Hydrolysis; D009943:Organophosphorus Compounds / Q000378:metabolism*; D012615:Sea Anemones / Q000378:metabolism*
D000596:Amino Acids; D006023:Glycoproteins; D009943:Organophosphorus Compounds; D000615:Aminoethylphosphonic Acid