title: Correlation analysis of electrical noise in lipid bilayer membranes: kinetics of gramicidin A channels.
abstract: If a membrane contains ion-conducting channels which form and disappear in a random fashion, an electric current which is passed through the membrane under constant voltage shows statistical fluctuations. Information on the kinetics of channel formation and on the conductance of the single channel may be obtained by analyzing the electrical noise generated in a membrane containing a great number of channels. For this purpose the autocorrelation function of the current noise is measured at different concentrations of the channel-forming substance. As a test system for the application of this technique we have used lipid bilayer membranes doped with gramicidin A. From the correlation time of the current noise generated by the membrane, the rate constants of formation (k-R) and dissociation (k-D) of the channels could be determined. In addition, the mean square of the current fluctuations yielded the single-channel conductance lambda. The values of k-R, k-D, and lambda obtained from the noise analysis agreed closely with the values determined by relaxation measurments and single-channel experiments.
mesh_terms: D001665:Binding Sites; D004553:Electric Conductivity; D006096:Gramicidin; D007700:Kinetics; D008433:Mathematics; D008567:Membranes, Artificial; D008954:Models, Biological; D010713:Phosphatidylcholines; D013696:Temperature; D013997:Time Factors
chemical_list: D008567:Membranes, Artificial; D010713:Phosphatidylcholines; D006096:Gramicidin