title: Use of a Giemsa stain to detect changes in acrosomes of frozen ram spermatozoa.
abstract: Acrosomal structures of ram spermatozoa were prominently stained when air dried smears of diluted semen were fixed for 15 minutes in buffered formal saline and stained for 90 minutes in a 6 per cent (v/v) buffered solution of Giemsa stain. Progressive disruption of the acrosomes was demonstrated during chilling and deep-freezing of the spermatozoa, and the degree of damage was systematically scored. A rapid and repeatable estimate of the state of the acrosomes in a sample could be made from the mean score of 20 spermatozoa examined per slide.
mesh_terms: D000177:Acrosome / Q000648:ultrastructure*; D000818:Animals; D004530:Egg Yolk; D004542:Ejaculation; D005260:Female; D005615:Freezing; D005947:Glucose / Q000494:pharmacology; D005990:Glycerol / Q000494:pharmacology; D008297:Male; D011309:Preservation, Biological; D012661:Semen / Q000166:cytology; D012756:Sheep / Q000033:anatomy & histology* / Q000502:physiology; D013094:Spermatozoa / Q000648:ultrastructure*; D013194:Staining and Labeling
chemical_list: D005947:Glucose; D005990:Glycerol