Impact of Ig-anti-Ig column fractionation on T-lymphocyte functions.
C57Bl/6 anti-Pe15-immune spleen cells were fractionated on immunoglobulin (Ig)-anti-Ig antibody columns in medium containing EDTA or in EDTA-free medium. The antigen-specific cytotoxic potential of immune cells in vitro was only slightly enriched after passage through the columns in the absence of EDTA, whereas immune cells passed through the columns in the presence of EDTA displayed a cytotoxic potential enriched about three times compared with unfractionated cells; this is similar to the enrichment in theta-positive cells. The lack of increase of the cytotoxic potential of immune cells after passage through the columns in the absence of EDTA was shown to be due to the adsorption of subpopulations of cytotoxic T lymphocytes. These cytotoxic cells could be eluted with EDTA-containing medium, and the findings suggest that their adsorption may be mediated via the interaction between cell-bound Fc receptors and the column antigen-antibody complexes.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis; D000917:Antibody Formation; D000936:Antigen-Antibody Complex; D002458:Cell Fractionation / Q000379:methods; D003601:Cytotoxicity Tests, Immunologic; D004492:Edetic Acid; D000939:Epitopes; D005260:Female; D007114:Immunization; D007141:Immunoglobulin Fc Fragments / Q000032:analysis; D007136:Immunoglobulins / Q000032:analysis; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D012709:Serum Albumin / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D000936:Antigen-Antibody Complex; D000939:Epitopes; D007141:Immunoglobulin Fc Fragments; D007136:Immunoglobulins; D012709:Serum Albumin; D004492:Edetic Acid