Unlinked control of multiple glucocorticoid-induced processes in HTC cells.
HTC cell variants chosen for their lack of tyrosine aminotransferase (EC 2.6.1.5) (TAT) induction by glucocorticoids were tested for interrelated effects on other glucocorticoid responses: TAT induction by dibutyryl cyclic AMP (dBcAMP) +/- dexamethasone, glutamine synthetase (GS) induction, cyclic nucleotide phosphodieterase (PDE) suppression, inhibition of alpha-aminoisobutyric acid (AIB) uptake, inhibition of plasminogen activator (PA), and induction of mouse mammary tumor virus (MTV). Loss of TAT induction by steroid was accompanied by loss of TAT induction by dBcAMP and of PDE suppression by steroid. In addition, subclones of MTV-infected cells were examined for the effect of the virus on glutamine synthetase (GS) and TAT induction. The virus had no effect on their induction in wild-type cells and no effect on GS induction in the variants. One MTV-infected subclone from a TAT variant, however, showed significant return of TAT induction.
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000378:metabolism; D000621:Aminoisobutyric Acids / Q000378:metabolism; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D003994:Bucladesine / Q000494:pharmacology; D002460:Cell Line; D002472:Cell Transformation, Viral; D000242:Cyclic AMP / Q000096:biosynthesis; D003907:Dexamethasone / Q000494:pharmacology*; D004790:Enzyme Induction / Q000187:drug effects; D005974:Glutamate-Ammonia Ligase / Q000096:biosynthesis*; D008114:Liver Neoplasms, Experimental; D008324:Mammary Tumor Virus, Mouse; D009154:Mutation; D010958:Plasminogen / Q000378:metabolism; D051381:Rats; D014444:Tyrosine Transaminase / Q000096:biosynthesis*
D000621:Aminoisobutyric Acids; D003994:Bucladesine; D003907:Dexamethasone; D010958:Plasminogen; D000242:Cyclic AMP; D014444:Tyrosine Transaminase; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D005974:Glutamate-Ammonia Ligase