An enzyme electrode for acetylcholine.
A new enzyme electrode is described to measure continuously acetylcholine concentration. A coating containing active acetylcholinesterase is produced on a pH-glass electrode. The mean thickness of the coating is 50 micrometer. Optimal operational conditions with respect to buffer concentration, ionic strength, linearity, stability, sensitivity, pH of the bulk solution, and response time are studied and discussed. The use of acetylcholinesterase-containing membranes as sensors could offer several novel advantages.
D000109:Acetylcholine / Q000032:analysis*; D000110:Acetylcholinesterase / Q000378:metabolism*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005898:Glass; D006863:Hydrogen-Ion Concentration; D008722:Methods
D004800:Enzymes, Immobilized; D000110:Acetylcholinesterase; D000109:Acetylcholine