An approach to quantitation in rhodamine isothiocyanate labeling.
A survey of commercially available TMRI and RBI products revealed that many are of unsatisfactory quality. Optimum dye/IgG ratios were obtained in conjugates with TMRI of about 65% purity by labeling them with reaction mixture ratios of 10 and 20 mug dye/mg IgG. Optimum labeling with RBI of comparable purity required reaction mixture ratios of at least 20 mug dye/mg IgG. Although specific storage stability studies of TMRI or RBI products were not performed, one TMRI product underwent appreciable degradation during a 2-year period of storage in a desiccator over Drierite. The importance of evaluating the protein labeling capacity of TMRI or RBI products before making immune conjugate preparations is evident.
D000818:Animals; D002848:Chromatography, DEAE-Cellulose; D005455:Fluorescent Antibody Technique / Q000379:methods*; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G; D007259:Infrared Rays; D011506:Proteins / Q000032:analysis; D011817:Rabbits; D012235:Rhodamines / Q000032:analysis*; D013056:Spectrophotometry, Ultraviolet; D013194:Staining and Labeling / Q000379:methods; D014966:Xanthenes / Q000032:analysis*
D007074:Immunoglobulin G; D011506:Proteins; D012235:Rhodamines; D014966:Xanthenes