title: Studies on vertebrate kidney. V. PAS-positive lipids in kidneys of fishes from different habitats.
abstract: Five fish species living in different habitats, i.e. fresh water, estuarine and marine, were studied for the distribution of PAS-positive materia in various regions of the kidney, 10 minutes' oxidation with 0-5 per cent. HIO4 being employed prior to treatment with Schiff's reagent. PAS-positive material was detected in different sites of the kidney, i.e. brush border of proximal tubules, proximal tubule cells' cytoplasm, distal tubule cells' cytoplasm, glomerulus, basal cell border of proximal tubules and the interstitial cells. Of these sites, the brush border of the proximal tubule of Scoliodon sorrakowah showed the presence of PAS-positive lipids. Elsewhere the PAS-positive reaction was due to carbohydrates. Free aldehyde groups were absent. In Tilapia mossambica and Labeo rohita, PAS staining was enhanced after chloroform-methanol extraction, particularly in the brush border of the proximal tubule. The significance of these findings is discussed.
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D005399:Fishes / Q000502:physiology*; D007668:Kidney / Q000032:analysis* / Q000166:cytology / Q000502:physiology; D008055:Lipids / Q000032:analysis*; D008297:Male; D013045:Species Specificity; D013194:Staining and Labeling
chemical_list: D008055:Lipids