Isolation of stable and unstable ("suppressed") Beta2-microglobulin-deficient clones from a lymphoid cell line.
After selection with anti-Beta2m and C against a human lymphoid line, about 10% of surviving clones manifested decreased cytotoxic sensitivity to anti-Beta2m("suppression") which gradually reverted to normal after 35 to 55 cell doublings. Antiserum alone induced suppression. Exposure of suppressed clones to DMSO substantially reversed suppression. One clone, surviving selection, had a stable decrease in anti-Beta2m sensitivity; it possibly represents a Beta2m mutation.
D001609:Beta-Globulins / Q000302:isolation & purification*; D002460:Cell Line; D002999:Clone Cells / Q000276:immunology; D003601:Cytotoxicity Tests, Immunologic; D006680:HLA Antigens; D006801:Humans; D007165:Immunosuppression Therapy; D008214:Lymphocytes / Q000276:immunology; D001613:beta 2-Microglobulin / Q000172:deficiency / Q000302:isolation & purification*
D001609:Beta-Globulins; D006680:HLA Antigens; D001613:beta 2-Microglobulin