Particle-associated RNA dependent DNA polymerase and high-molecular-weight RNA in a human cell line derived from polycythemia vera bone marrow.
A particle fraction with a density of 1.15-1.19 g/cm3 was isolated from the cytoplasm of a human cell line established in culture from the bone marrow of an untreated patient with polycythemia vera. Electron micrographs of cross sections of cells and cell homogenates revealed virus-like particles on which DNA could be synthesized. An RNA-dependent DNA polymerase, isolated from the particles, preferred poly(rA)-oligo(dT) over poly(dA)-oligo(dT) and was able to polymerize deoxyguanosine monophosphate in a reaction stimulated by poly(rC)-oligo(dG).
D001853:Bone Marrow / Q000378:metabolism* / Q000648:ultrastructure; D002460:Cell Line; D003593:Cytoplasm / Q000201:enzymology / Q000378:metabolism; D006150:Guanine Nucleotides / Q000378:metabolism; D006801:Humans; D009838:Oligodeoxyribonucleotides; D009858:Oncogenic Viruses / Q000378:metabolism; D011061:Poly A; D011066:Poly C; D011068:Poly G; D011087:Polycythemia Vera / Q000378:metabolism* / Q000382:microbiology; D012367:RNA, Viral / Q000378:metabolism*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*; D013698:Templates, Genetic; D013942:Thymine Nucleotides
D006150:Guanine Nucleotides; D009838:Oligodeoxyribonucleotides; D012367:RNA, Viral; D013942:Thymine Nucleotides; D011061:Poly A; D011068:Poly G; D011066:Poly C; D012194:RNA-Directed DNA Polymerase