Bacteriophage Mu-1-induced mutation to mutT in Escherichia coli.
Of approximately 10,000 independent phage Mu-1 lysogens, 3 had a mutator phenotype. One (mutation designated mut-49) resembled mutT1 in the frequency and types of mutations induced. mut-49 was mapped between leu and ace and was not separable from the Mu prophage. mut-49 was recessive and did not complement mutT1. mut-49, like mutT1, did not increase the reversion of the frameshift mutation lac Z (ICR48). mut-49 and mutT1 induced the same two classes of trpA78 revertants, indicating that mut-49 induced adenine-thymine leads to cytosine-guanine transversions. The results support previous work indicating that the mutational specificity of mutT is gene and not allele specific.
D001386:Azides / Q000494:pharmacology; D002874:Chromosome Mapping; D003090:Coliphages / Q000254:growth & development*; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism; D008242:Lysogeny; D009154:Mutation; D010641:Phenotype; D010626:Phenylethyl Alcohol / Q000494:pharmacology; D013307:Streptomycin / Q000494:pharmacology; D014161:Transduction, Genetic; D014364:Tryptophan / Q000031:analogs & derivatives / Q000494:pharmacology
D001386:Azides; D014364:Tryptophan; D010626:Phenylethyl Alcohol; D013307:Streptomycin