A rapid method for preparing tissue culture clones for light and electron microscopy.
Fixation and epoxy-embedment of tissue culture clones in situ were carried out in Falcon tissue culture plates. The clone of cells, retained at one end of the casting, was stained with azure II-methylene blue and then studied with the oil immersion objective. The dimensions of the epoxy casting were ideal for mouting as a block in conventional ultramicrotone chucks. The use of one epoxy casting permits a single preparation of tissue culture clones for direct light microscopic observations and subsequently for ultramicrotomy.
D002999:Clone Cells; D046508:Culture Techniques / Q000379:methods*; D008751:Methylene Blue; D008853:Microscopy; D008854:Microscopy, Electron; D013194:Staining and Labeling
D008751:Methylene Blue