DNA polymerase activity in plasma from leukaemic guinea-pigs.
Measurement of DNA polymerase in leukaemic guinea-pig plasms reveals the presence of low levels of sedimentable and non-sedimentable enzymic activities. Since the sedimentable DNA polymerase is ribonuclease sensitive, uses poly(C).oligo(dG) as template, and bands in a sucrose density gradient at 1-17 g/ml it is thought to be the GPLV-associated reverse transcriptase. The soluble DNA polymerase is stimulated by ribonuclease and is probably of cellular origin.
D000818:Animals; D002499:Centrifugation, Density Gradient; D004254:DNA Nucleotidyltransferases / Q000097:blood*; D004789:Enzyme Activation; D006168:Guinea Pigs; D007942:Leukemia, Experimental / Q000097:blood / Q000201:enzymology*; D011061:Poly A; D011066:Poly C; D011071:Poly T; D012194:RNA-Directed DNA Polymerase / Q000097:blood; D012260:Ribonucleases; D013997:Time Factors
D011061:Poly A; D011071:Poly T; D011066:Poly C; D004254:DNA Nucleotidyltransferases; D012194:RNA-Directed DNA Polymerase; D012260:Ribonucleases