Kinetic immunoturbidimetry: the estimation of albumin.
The applicability of commercially available antisera for use in kinetic immunoturbidimetry has been studied using the protein albumin as a simple model. The sensitivity of the kinetic immunoturbidimetric approach has been found to be comparable to published data for nephelometric systems, being able to detect concentrations of protein as low as 1 mg/l. The technique has shown to be as precise as most dye binding techniques for the measurement of albumin, producing a within batch C.V. of 1.0% and a between batch C.V. of 1.5%. The technique was found to be comparable to another immunological technique (RID). The fast assay time (30--60 sec), cost and good precision makes this the method of choice for routine albumin measurement. The extreme sensitivity of the technique of kinetic immunoturbidimetry makes the technique applicable to the estimation of a wide range of proteins in blood, urine and CSF.
D004396:Coloring Agents; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007106:Immune Sera / Q000032:analysis; D007120:Immunochemistry; D005779:Immunodiffusion; D007700:Kinetics; D009391:Nephelometry and Turbidimetry / Q000379:methods; D011108:Polymers; D012709:Serum Albumin / Q000032:analysis*; D012965:Sodium Chloride; D013696:Temperature
D004396:Coloring Agents; D007106:Immune Sera; D011108:Polymers; D012709:Serum Albumin; D012965:Sodium Chloride