title: RNA-dependent DNA polymerase activity of RNA tumor viruses. V. Rous sarcoma virus single-stranded RNA-DNA covalent hybrids in infected chicken embryo fibroblast cells.
abstract: RNA-DNA covalent hybrids containing viral RNA have been isolated from nuclear fractions of Rous sarcoma virus-infected chicken embryo fibroblast cells shortly after virus infection. The formation of covalent hybrid structures depends upon a functional reverse transcriptase in vivo, since its appearance in cells is temperature dependent when infected with Rous sarcoma virus mutant LA335, which contains a temperature-sensitive reverse transcriptase.
mesh_terms: D000818:Animals; D001358:Avian Sarcoma Viruses / Q000254:growth & development*; D002500:Centrifugation, Isopycnic; D002501:Centrifugation, Zonal; D002642:Chick Embryo; D004247:DNA; D005347:Fibroblasts; D009154:Mutation; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral; D012194:RNA-Directed DNA Polymerase; D013696:Temperature; D014316:Tritium; D014779:Virus Replication
chemical_list: D012367:RNA, Viral; D014316:Tritium; D004247:DNA; D012194:RNA-Directed DNA Polymerase