Immunochemical study of beta-glucuronidase inhibitor from porcine sublingual gland. Interaction of beta-glucuronidase inhibitor with alpha2-macroglobulin.
Antiserum to the inhibitor of beta=glucuronidase isolated from porcine sublingual gland was prepared in rabbits. Double immunodiffusion with the inhibitor produced a single precipitin line. However, neutralization of the inhibitor was produced by the antiserum and also by normal serum. Anti-beta-glucuronidase inhibitor isolated from human serum, by fractionation with (NH4)2 SO4 followed by DEAE-cellulose, Sephadex G-200 and Sepharose 4B chromatography, was identified as alpha2-macroglobulin by using ultracentrifuge analysis and immunoelectrophoresis. The mechanism of interaction of beta-glucuronidase inhibitor with alpha2-macroglobulin was also studied.
D000818:Animals; D005966:Glucuronidase / Q000037:antagonists & inhibitors*; D006023:Glycoproteins / Q000494:pharmacology*; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D011506:Proteins / Q000032:analysis / Q000276:immunology; D011817:Rabbits / Q000276:immunology; D013361:Sublingual Gland / Q000032:analysis*; D013552:Swine; D000511:alpha-Macroglobulins
D006023:Glycoproteins; D011506:Proteins; D000511:alpha-Macroglobulins; C011473:beta-glucuronidase inhibitor; D005966:Glucuronidase