Determination of antibody avidity at the cellular level by the plaque inhibition technique: effect of valence of the inhibitor.
Inhibition of plaque formation by multivalent and univalent ligands was compared as an assay of avidity of antibody produced by PFC. Multivalent ligands are much more effective as inhibitors and their use tends to impart an appearance of lack of heterogeneity and high avidity to the PFC populations being studied. It is thus probably generally advisable to employ univalent ligands in such studies.
D000614:Aminocaproates; D000818:Animals; D000917:Antibody Formation; D000921:Antibody-Producing Cells; D000937:Antigen-Antibody Reactions; D002417:Cattle / Q000276:immunology; D002645:Chickens / Q000276:immunology; D004140:Dinitrophenols / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology; D006462:Hemolytic Plaque Technique; D006801:Humans; D007106:Immune Sera; D008198:Lymph Nodes / Q000166:cytology; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D008722:Methods; D051379:Mice; D010047:Ovalbumin; D011817:Rabbits / Q000276:immunology; D012709:Serum Albumin; D012756:Sheep / Q000276:immunology; D005719:gamma-Globulins
D000614:Aminocaproates; D004140:Dinitrophenols; D007106:Immune Sera; D012709:Serum Albumin; D005719:gamma-Globulins; D010047:Ovalbumin