Biochemical characteristics of rat C-type virus WF-1.
The non-oncogenic rat C-type virus WF-1, isolated from a Wistar-Furth rat embryo cell line, was characterized biochemically. The purified virus has a buoyant density of 1.15 to 1.16 g/cm3 in sucrose, RNA-dependent DNA polymerase (reverse transcriptase) activity, and RNA with a sedimentation coefficient of 62 to 68 S. The viral RNA is single-stranded, and, upon treatment with heat, yields components with sedimentation coefficients of 36 S, 18 to 20 S, and 4 to 12 S.
D000818:Animals; D002499:Centrifugation, Density Gradient; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D006358:Hot Temperature; D008970:Molecular Weight; D012367:RNA, Viral / Q000032:analysis; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D051381:Rats / Q000382:microbiology*; D012190:Retroviridae / Q000032:analysis* / Q000201:enzymology
D012367:RNA, Viral; D012194:RNA-Directed DNA Polymerase; D004121:Dimethyl Sulfoxide