[Further improvement of the ACD-AG protective solution for blood. II. Behavior of the oxygen affinity of preserved blood in ACD-AG medium and in a protective solution with addition of pyruvate and xylitol and elevated Ph values].
The present paper deals with the behaviour of the oxygen transport function as well as the 2.3 DPG and ATP levels of erythrocytes during the storage in an ACD-AG solution. In the ACD-AG blood in P 50 fell from 20mm of Hg to values of 12 mm of Hg within 4 weeks of storage. The 2.3 DPG content had already fallen to values below 10% within a fortnight. Additions of xylitol (10 mM in the blood) and pyruvate (0.3 mM in the blood) will delay the decrease of P 50 and the 2.3 DPG content. Concerning the ATP behaviour there was no significant difference between ACD-AG blood and that with additions of xylitol and pyruvate. Up to a storage of a fortnight stored blood in the ACD-AG solution of xylitol and pyruvate will be equal to ACD-AG fresh blood as far as the parameter of oxygen transport function is concerned.
D000255:Adenosine Triphosphate / Q000097:blood; D001692:Biological Transport; D001793:Blood Preservation; D002951:Citrates; D003451:Cryoprotective Agents; D004163:Diphosphoglyceric Acids / Q000097:blood; D005947:Glucose; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010100:Oxygen / Q000378:metabolism; D011773:Pyruvates / Q000494:pharmacology; D014993:Xylitol / Q000494:pharmacology
D002951:Citrates; D003451:Cryoprotective Agents; D004163:Diphosphoglyceric Acids; D011773:Pyruvates; D000255:Adenosine Triphosphate; D005947:Glucose; D010100:Oxygen; D014993:Xylitol
Weitere Optimierung der ACD-AG-Konservierungslösung für Blut. II. Mitteilung: Verhalten der Sauerstoffaffinität des Konservenblutes im ACD-AG-Medium und einer Konservierungslösung mit Pyruvat- und Xylitzusatz sowie erhöhtem pH-Wert