title: Augmented uptake of neuraminidase-treated sheep red blood cells: participation of opsonic factors.
abstract: Neuraminidase from Vibrio cholerae (VCN) was used to treat sheep red blood cells (SRBC) which were then incubated in vitro with murine peritoneal macrophages. The uptake of VCN-treated SRBC by macrophages was greater than the uptake of SRBC not treated with VCN. SRBC opsonized with normal mouse serum (NMS) were taken up to a greater extent than untreated SRBC. SRBC treated with VCN and opsonized with NMS were phagocytosed to a greater extent than untreated SRBC, VCN-treated SRBC, or opsonized SRBC. Evidence demonstrated that factors in serum from normal C3H/HeJ mice augmented the uptake of VCN-treated SRBC in greater amounts than of normal SRBC. These findings were discussed in relation to the increased immunogenicity of neuraminidase-treated cells.
mesh_terms: D000818:Animals; D000939:Epitopes; D004912:Erythrocytes / Q000187:drug effects* / Q000276:immunology; D005260:Female; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H; D009439:Neuraminidase / Q000494:pharmacology*; D009895:Opsonin Proteins; D010587:Phagocytosis; D012756:Sheep; D014734:Vibrio cholerae / Q000201:enzymology
chemical_list: D000939:Epitopes; D009895:Opsonin Proteins; D009439:Neuraminidase