A p-nitrophenyl alpha-galactoside hydrolase from Pseudomonas atlantica. Localization of the enzyme.
A p-nitrophenyl alpha-galactoside hydrolase is partially released when whole cells of Pseudomonas atlantica are converted to spheroplasts. The p-nitrophenyl alpha-glactoside hydrolase is completely inactivated by treatment of whole cells with diazonaphthalene -- disulfonic acid (NDS), a reagent which does not penetrate the cytoplasmic membrane. Under the conditions used no inactivation of lactic acid dehydrogenase was observed. A specific staining procedure for this enzyme for use in electron microscopy was developed. The results with this technique in conjunction with the results of spheroplasting and NDS localization suggest that p-nitrophenyl alpha-galactoside hydrolase is located in or on the double-track membranes, primarily on the outer double track.
D002462:Cell Membrane / Q000201:enzymology; D002474:Cell-Free System; D006026:Glycoside Hydrolases / Q000302:isolation & purification* / Q000378:metabolism; D007202:Indicators and Reagents / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008854:Microscopy, Electron; D009282:Naphthalenesulfonates / Q000494:pharmacology; D009598:Nitrophenylgalactosides; D011549:Pseudomonas / Q000201:enzymology* / Q000648:ultrastructure; D013104:Spheroplasts / Q000201:enzymology / Q000648:ultrastructure; D013194:Staining and Labeling
D007202:Indicators and Reagents; D009282:Naphthalenesulfonates; C011442:diazonaphthalenedisulfonic acid; D009598:Nitrophenylgalactosides; D007770:L-Lactate Dehydrogenase; D006026:Glycoside Hydrolases