[Presence of the H blood group antigen on a carcinoembryonic antigen (CEA). Enzymatic transformation of H to A and B specificities].
The carcinoembryonic antigen (CEA-M) was purified from a hepatic metastasis obtained from a blood group O patient with a cancer of the rectum. Using 125I-labelled-CEA and blood group antisera, H specificity was found on the CEA-M; the addition of anti-H to anti-CEA does not modify the binding of labelled-CEA-M to its antibodies (86%), this result leads us to conclude that H and CEA determinants are carried by the same molecule. However the low percentage of binding (30% with 1/10 anti-H) suggests that only a few CEA-M molecules do carry the H antigenic determinant. Finally, glycosyltransferases were used to modify the H specificity into blood group A and B specificities.
D000017:ABO Blood-Group System; D000918:Antibody Specificity; D002272:Carcinoembryonic Antigen / Q000032:analysis*; D003429:Cross Reactions; D000939:Epitopes; D005646:Fucosyl Galactose alpha-N-Acetylgalactosaminyltransferase / Q000378:metabolism; D006801:Humans; D008113:Liver Neoplasms / Q000097:blood / Q000276:immunology; D014539:Uridine Diphosphate Sugars
D000017:ABO Blood-Group System; D002272:Carcinoembryonic Antigen; D000939:Epitopes; D014539:Uridine Diphosphate Sugars; D005646:Fucosyl Galactose alpha-N-Acetylgalactosaminyltransferase
Présence de l'antigène H de groupe sanguin sur un antigène carcino-embryonnaire (CEA). Modification enzymatique de la spécificité H en spécificité A et B