Agarose gel electrophoresis of plasma lipoproteins using the Durrum cell.
1. A low cost method for lipoprotein electrophoresis in agarose gel is described. The basic procedure, described by Noble, has been modified for use with a Durrum cell which is commonly used for paper electrophoreis. 2. Up to 28 samples can be analyzed per Durrum cell in 4 hours. 3. A staining technique, which involves filtration of a suspension of Sudan Black B, allows for rapid staining and provides satisfactory reproducibility from run to run. 4. In a small number of patients a double pre-beta band is seen, and is different from sinking pre-beta and floating beta lipoproteins.
D004586:Electrophoresis / Q000295:instrumentation; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood; D008074:Lipoproteins / Q000097:blood*; D008075:Lipoproteins, HDL / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood; D008079:Lipoproteins, VLDL / Q000097:blood; D012685:Sepharose; D013194:Staining and Labeling
D008074:Lipoproteins; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D012685:Sepharose