Phosphorylation of Isocitrate dehydrogenase of Escherichia coli.
Addition of acetate to a stationary phase culture of Escherichia coli in glycerol mineral salts medium containing phosphorus-32-labeled orthophosphate results in rapid loss of isocitrate dehydrogenase activity and concomitant incorporation of phosphorus-32 into the enzyme. This is the first example of protein phosphorylation in a bacterium in which the endogenous substrate for the protein kinase has been identified.
D000085:Acetates / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology*; D007521:Isocitrate Dehydrogenase / Q000378:metabolism*; D009249:NADP / Q000378:metabolism; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism; D012694:Serine / Q000378:metabolism; D013912:Threonine / Q000378:metabolism
D000085:Acetates; D013912:Threonine; D012694:Serine; D009249:NADP; D007521:Isocitrate Dehydrogenase; D011494:Protein Kinases