A method for determining plasma proteins in inflammatory exudate absorbed by Sephadex.
Dry cross-linked dextran (Sephadex pharmaceutical grade, Debrisan) was applied to weeping sores. After some exudate had been absorbed the gels were removed. The amount of exudate was determined by isotope dilution techniques. The accuracy of the method was tested by determination of plasma proteins in samples of diluted serum containing known concentrations of some plasma proteins. The method seems suitable for determining various high and low molecular weight constituents of exudates.
D000042:Absorption; D001798:Blood Proteins / Q000032:analysis*; D002056:Burns / Q000097:blood; D002850:Chromatography, Gel; D003911:Dextrans; D005122:Exudates and Transudates / Q000032:analysis*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007871:Leg Ulcer / Q000097:blood; D009961:Orosomucoid / Q000032:analysis; D012709:Serum Albumin / Q000032:analysis; D014946:Wound Infection / Q000097:blood*; D000511:alpha-Macroglobulins / Q000032:analysis
D001798:Blood Proteins; D003911:Dextrans; D007074:Immunoglobulin G; D009961:Orosomucoid; D012709:Serum Albumin; D000511:alpha-Macroglobulins