[Immunochemical study of the antigenic structure of bacteria of genus Bordetella. IV. Fractionation of B. pertussis extracts and study of the immunochemical and biological properties of the isolated fractions].
Fractionation of an extract of pertussis microbes was carried out with the aid of gel-filtration on Sephadex G-100, ion-exchange chromatography on DEAE-cellulose, and preparative electrophoresis. Fractions differing in serological, immunogenic activity and the content of antigenic components were isolated. In using the method of gel-filtration of sefadex G-100 the greatest serological, immunogenic and histamine-sensitizing activity was possessed by the high-molecular fraction containing 8 of 11 antigenic components detected in the initial extract. The antigenic components were distributed into 5 fractions by the ion exchange chromatography on DEAE-cellulose. The greatest serological activity was possessed by fractions exiting from the column at the 0.01--0.04M interval of the phosphate buffer concentration. A method of preparative electrophoresis from the pertussis microbes extract was applied and two fractions were isolated from the anode and the cathode zones, each containing 4 antigenic components only, but possessing serological and immunogenic activity and having no histamine-sensitizing properties.
D000818:Animals; D000942:Antigens, Bacterial / Q000302:isolation & purification / Q000633:toxicity; D001886:Bordetella pertussis / Q000276:immunology*; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004587:Electrophoresis, Agar Gel; D006636:Histamine Release; D007928:Lethal Dose 50; D051379:Mice
D000942:Antigens, Bacterial
Immunokhimicheskoe issledovanie antigennoĭ struktury mikrobov roda Bordetella. Soobshchenie IV. Fraktsionirovanie ékstraktov kokliushnykh mikrobov i izuchenie immunokhimicheskikh i biologicheskikh svoĭstv vydelennykh fraktsiĭ