Comparison of radio-immunoassay and lymphocytotoxicity inhibition techniques for the determination of beta2 microglobulin.
Rabbit anti-human beta2 microglobulin antisera can lyse human lymphocytes in the presence of rabbit complement. Inhibition of the lymphocytotoxic reaction by highly purified beta2m was applied to the measurement of beta2m concentration in biological fluids. Parallel determinations were also performed using a radioimmunoassay. Lymphocytotoxicity inhibition is simple and more sensitive than radial immunodiffusion but less sensitive than the radioimmunoassay. beta2m was measured by these two techniques in serum and urine from normal individuals, uremic or transplanted patients.
D001609:Beta-Globulins / Q000032:analysis*; D003601:Cytotoxicity Tests, Immunologic; D006801:Humans; D011863:Radioimmunoassay; D001613:beta 2-Microglobulin / Q000032:analysis* / Q000652:urine
D001609:Beta-Globulins; D001613:beta 2-Microglobulin