Isoelectric focusing of cells using zwitterionic buffers.
A simple method of isoelectric focusing of cells is described. The pH gradient, superimposed on a density gradient, is developed by generating opposing concentration gradients of two zwitterionic buffers. The method can be used as a cell separation technique or as a means of characterizing the cell type on the basis of the focusing pH. Focusing is rapid and thus the method is of special advantage in its application to cells.
D000665:Ampholyte Mixtures; D000818:Animals; D002021:Buffers; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D002642:Chick Embryo; D046508:Culture Techniques; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D051381:Rats
D000665:Ampholyte Mixtures; D002021:Buffers