Biophysical properties of a non-cultivable 29-nm enteric virus.
A 29 nm non-cultivable virus (NCV) was detected in faecal extracts from children hospitalized for gastroenteritis. The NCV had a density of 1.35 g/ml in glycerol-potassium tartrate density gradients and was resistant to degradation by proteolytic enzymes, non-ionic detergents and pH extremes. The surface of these virus particles had knob-like projections which appeared to have a symmetrical arrangement. When heated to 56 degrees C, the virus was completely degraded to soluble components which could not be seen by electron microscopy.
D000328:Adult; D000914:Antibodies, Viral / Q000032:analysis; D003126:Colostrum / Q000276:immunology; D005243:Feces / Q000382:microbiology; D005260:Female; D005759:Gastroenteritis / Q000382:microbiology*; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007223:Infant; D011247:Pregnancy; D012401:Rotavirus / Q000302:isolation & purification; D014713:Viruses, Unclassified / Q000276:immunology / Q000302:isolation & purification* / Q000648:ultrastructure
D000914:Antibodies, Viral