Chemical composition and immunological specificity of cell wall polysaccharide group antigens of streptococcal groups P and U.
The group antigens of streptococcal groups P and U were extracted with formamide and purified on diethylaminoethyl-Sephadex A-25 and Sephadex G-200. The antigens were shown to be polysaccharides located in the cell walls of the organisms. In a precipitin test, the P and U group antigens did not cross-react with homologous sera of each other, nor with specific antiserum of the group antigen of group E streptococci. The polysaccharide comprising the group P antigen contained rhamnose, glucose, galactose, glucosamine, and galactosamine; the group U antigen was similar in composition but lacked galactosamine and contained more galactose.
D001426:Bacterial Proteins / Q000032:analysis; D001431:Bacteriological Techniques; D002473:Cell Wall / Q000276:immunology*; D002850:Chromatography, Gel; D002854:Chromatography, Paper; D000939:Epitopes; D005559:Formamides; D005690:Galactose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D006595:Hexosamines / Q000032:analysis; D006868:Hydrolysis; D005779:Immunodiffusion; D010758:Phosphorus / Q000032:analysis; D011135:Polysaccharides, Bacterial / Q000032:analysis; D011233:Precipitin Tests; D012210:Rhamnose / Q000032:analysis; D013291:Streptococcus / Q000276:immunology*
D001426:Bacterial Proteins; D000939:Epitopes; D005559:Formamides; D006595:Hexosamines; D011135:Polysaccharides, Bacterial; D010758:Phosphorus; D005947:Glucose; D012210:Rhamnose; D005690:Galactose