Increased levels of adenosine 3',5'-cyclic monophosphate in Tetrahymena stimulated by glucose and mediated by Ca2+ and epinephrine.
Stationary phase Tetrahymena cells exposed to 0.5-1% glucose experience an 8-fold increase in intracellular cyclic AMP within 1 hr. The increase follows an enhanced uptake of Ca2+ triggered by the glucose addition. Inhibiting the Ca2+ influx with ethylene glycol bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid blocks the increase in cyclic AMP, but the block can be reversed by the addition of epinephrine. Both this epinephrine effect and the glucose-induced increase in cyclic AMP are counteracted by the beta-adrenergic inhibitor dichloroisoproterenol.
D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D000242:Cyclic AMP / Q000378:metabolism*; D004837:Epinephrine / Q000494:pharmacology*; D005947:Glucose / Q000494:pharmacology*; D007545:Isoproterenol / Q000494:pharmacology; D007700:Kinetics; D013769:Tetrahymena pyriformis / Q000187:drug effects / Q000378:metabolism*
D000242:Cyclic AMP; D005947:Glucose; D007545:Isoproterenol; D002118:Calcium; D004837:Epinephrine