Effect of trichloroacetic acid treatment on certain properties of spores of Bacillus cereus T.
Spores of Bacillus cereus T treated with trichloroacetic acid (6.1--61.2 mM) were compared with untreated spores, and as the concentration of the chemical increased, the following alterations in spore properties were found: (1) the extent of germination decreased irrespective of the germination medium used; (2) the spores became sensitive to sodium hydroxide (1 N) and hydrochloric acid (0.27 N), but not to lysozyme (200 micrograms/ml); (3) loss of dipicolinate increased on subsequent heating; and (4) the spores became more sensitive to heat. However, trichloroacetic acid-treated spores were still viable and there was no significant change in spore components. The mechanism of action of trichloroacetic acid is discussed.
D001409:Bacillus cereus / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D004352:Drug Resistance, Microbial; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D010848:Picolinic Acids / Q000378:metabolism; D012972:Sodium Hydroxide / Q000494:pharmacology; D013171:Spores, Bacterial / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D014238:Trichloroacetic Acid / Q000494:pharmacology*
D010848:Picolinic Acids; D012972:Sodium Hydroxide; D014238:Trichloroacetic Acid