Electron microscopy of tobacco mosaic virus prepared with the aid of negative staining-carbon film techniques.
The negative staining-carbon technique has been applied to suspensions of freshly prepared type strain tobacco mosaic virus particles in high concentrations. Electron microscope images show that paracrystalline arrays of the virus were formed, in which large areas of rods could be viewed along their long axes and in parallel arrays in horizontal positions. High-resolution micrographs showed the protein structure units in rods photographed in both vertical and horizontal orientations.
D003461:Crystallography; D003903:Deuterium; D008854:Microscopy, Electron; D008982:Molybdenum; D013194:Staining and Labeling / Q000379:methods*; D014027:Tobacco Mosaic Virus / Q000648:ultrastructure*; D014764:Viral Proteins
D014764:Viral Proteins; D008982:Molybdenum; D003903:Deuterium