Serological diagnosis of pneumococcal disease with enzyme-linked immunosorbent assay (ELISA).
Antibody response to pneumococcal type-specific polysaccharide was measured with enzyme-linked immunosorbent assay (ELISA). The test was shown to be up to 500 times more sensitive than indirect hemagglutination using chromic chloride-treated red blood cells. In 16/17 patients with pneumococcal pneumonia a significant antibody increase was seen as measured with ELISA. Only 6/23 patients with pneumonia caused by Mycoplasma pneumoniae gave a pneumococcal antibody rise and in those cases the increase was very slight. The authors consider ELISA a valuable contribution to the serological diagnosis of pneumococcal disease.
D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000032:analysis; D000942:Antigens, Bacterial; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D006386:Hemagglutination Tests; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D008875:Middle Aged; D011018:Pneumonia, Pneumococcal / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D011135:Polysaccharides, Bacterial / Q000032:analysis; D013296:Streptococcus pneumoniae / Q000276:immunology
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011135:Polysaccharides, Bacterial