title: Kinetics of antibody response to Ehrlichia canis assayed by the indirect fluorescent antibody method.
abstract: The kinetics of antibody production response to experimentally induced infection of dogs with Ehrlichia canis was determined by ion-exchange and molecularsieve chromatography and by indirect fluorescent antibody (IFA) test. The first IFA antibody at 7 days after inoculation resided in immunoglobulin M (IgM) and immunoglobulin A (IgA) classes. At approximately 21 days after inoculation, the antibody was in IgM, IgA, and immunoglobulin G (IgG) classes. Thereafter, antibody concentrations continued to increase in the IgG class; those in the other 2 immunoglobulin classes had a variable pattern. In 2 dogs which died 60 and 114 days after inoculation, a decrease of antibody concentration in the 3 immunoglobulin classes was evident at the time of death. In the carrier dog, however, which was killed 147 days after inoculation, antibody concentrations sustained increasing titers in the 3 immunoglobulin classes.
mesh_terms: D000818:Animals; D000917:Antibody Formation; D001772:Blood Cell Count; D001792:Blood Platelets; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004283:Dog Diseases / Q000097:blood / Q000276:immunology*; D004285:Dogs; D004536:Ehrlichia / Q000276:immunology*; D004906:Erythrocyte Count; D005455:Fluorescent Antibody Technique; D006400:Hematocrit; D007122:Immunoelectrophoresis; D007070:Immunoglobulin A / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007958:Leukocyte Count; D012287:Rickettsiaceae / Q000276:immunology*; D012288:Rickettsiaceae Infections / Q000097:blood / Q000276:immunology / Q000662:veterinary*; D018805:Sepsis / Q000097:blood / Q000276:immunology / Q000662:veterinary*; D005719:gamma-Globulins / Q000032:analysis
chemical_list: D007070:Immunoglobulin A; D007075:Immunoglobulin M; D005719:gamma-Globulins