Common antigenic structures of HL-A antigens. VI. Common antigenic determinants located on the 33,000 Dalton alloantigenic fragment portion of papain-solubilized HL-A molecules.
HL-A 33,000 Dalton fragments were isolated from three papain-solubilized HL-A preparations carrying different HL-A allospecificity by the dissociation induced in the reaction with rabbit antibodies to HL-A 11,000-Dalton fragment (i.e. human beta2-microglobulin). By assaying the binding activity with rabbit antisera against papain-solubilized HL-A molecules and against each of the HL-A component fragments and also with a battery of HL-A alloantisera, the 'antibody-dissociated' 33,000-Dalton fragments were shown to retain not only the HL-A alloantigenic determinants but also the HL-A common antigenic determinants that are native to the 33,000-Dalton fragment portion of undissociated HL-A molecules and to be devoid of the cryptic HL-A common antigenic determinants that are found on HL-A 33,000-Dalton fragments obtained by dissociation with chemical reagents including acid. However, on exposure to acid, the antibody-dissociated 33,000-Dalton fragments were found to lose their HL-A alloantigenic and common antigenic characteristics and gain the cryptic HL-A common antigenic determinants. The greater part of the HL-A common antigenic determinants found here appears to be primate cross-reacting determinants.
D000818:Animals; D000910:Antibodies, Heterophile; D000937:Antigen-Antibody Reactions; D003429:Cross Reactions; D000939:Epitopes; D006680:HLA Antigens / Q000032:analysis*; D000882:Haplorhini; D006649:Histocompatibility Antigens / Q000032:analysis*; D006801:Humans; D007518:Isoantibodies; D008253:Macaca mulatta; D051379:Mice; D008970:Molecular Weight; D010206:Papain; D051381:Rats; D012995:Solubility
D000910:Antibodies, Heterophile; D000939:Epitopes; D006680:HLA Antigens; D006649:Histocompatibility Antigens; D007518:Isoantibodies; D010206:Papain