Stereological study of endoplasmic reticulum, Golgi complex and secretory granules in the B-cells of normal and alloxan-treated mice.
Different B-cell organelles (lamellar and vesicular endoplasmic reticulum, Golgi complex, whole secretory granules and secretory granule cores) were studied stereologically in pancreatic islets from control mice and mice killed 10 or 60 min following alloxan injection. Ten min following alloxan a significant decrease was observed in the volume, surface and numerical densities of whole secretory granules and their cores, and a significant increase was found in the volume and surface densities of vesicular endoplasmic reticulum. At the 60 min observation time, a significant decrease was seen in the volume density of lamellar endoplasmic reticulum and Golgi complex, and in the volume, surface and numerical densities of whole secretory granules and their cores, and a significant increase was observed in the volume and surface densities of vesicular endoplasmic reticulum, and in the mean values for volume and surface of whole secretory granules and their cores. The stereological data indicate swelling of endoplasmic reticulum, decreased Golgi area, and decreased number and total volume and surface of secretory granules during  the first hour after alloxan administration to mice. The observations may be consistent with inhibited insulin synthesis.
D000496:Alloxan / Q000494:pharmacology; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000473:pathology*; D004721:Endoplasmic Reticulum / Q000187:drug effects / Q000648:ultrastructure*; D006056:Golgi Apparatus / Q000187:drug effects / Q000648:ultrastructure*; D007515:Islets of Langerhans / Q000187:drug effects / Q000648:ultrastructure*; D051379:Mice; D008854:Microscopy, Electron; D013997:Time Factors
D000496:Alloxan