[Studies of the antibody heterogeneity in carp (Cyprinus carpio L.). I. Electrophoretic and isoelectric spectra of anti-DNP-antibodies].
Carp IgM as well as carp anti-DNP-antibodies migrate electrophoretically very well as a diffuse band into polyacrylamide gel of large pore size. The isoelectric spectra of the carp anti-DNP-antibodies are heterogeneous and show bands in the pI-range of 4.0 to 6.4. The activity of focused anti-DNP-antibodies could be demonstrated in the pI range between 5.4 to 6.4 even in high antibody dilutions. The investigated structural heterogeneity of the anti-DNP-antibodies of carp is a further proof for the phylogenetically early onset of a large antibody heterogeneity of lower vertebrates.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000918:Antibody Specificity; D002347:Carps / Q000276:immunology*; D003530:Cyprinidae / Q000276:immunology*; D004136:Dinitrobenzenes / Q000276:immunology*; D006241:Haptens / Q000276:immunology; D006863:Hydrogen-Ion Concentration; D007122:Immunoelectrophoresis; D007075:Immunoglobulin M / Q000032:analysis*; D007525:Isoelectric Focusing; D009578:Nitrobenzenes / Q000276:immunology*
D000906:Antibodies; D004136:Dinitrobenzenes; D006241:Haptens; D007075:Immunoglobulin M; D009578:Nitrobenzenes
Untersuchungen zur Antikörperheterogenität beim Karpfen (Cyprinus carpio L.). I. Elektrophoretische und isoelektrische Spektren von Anti-DNP-Antikörpern.