A medium for commercial production of the halophilic Micrococcus nuclease.
A simple synthetic medium (glutamate-sucrose medium) was devised for production, during growth in shaken flasks, of extracellular halophilic nuclease (nuclease H) by a moderate halophile, Micrococcus varians subsp. halophilus. A simple medium consisting of 0.7% ammonium sulfate, 1.0% glucose, minerals, three vitamins, and 2 M NaCl gave good growth and excellent production of nuclease H in a jar fermentor when the pH was adjusted to 7.5 to 8.0 during cultivation.
D003470:Culture Media; D005092:Exonucleases / Q000096:biosynthesis*; D005095:Exoribonucleases; D005971:Glutamates / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008837:Micrococcus / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology; D013395:Sucrose / Q000378:metabolism
D003470:Culture Media; D005971:Glutamates; D012965:Sodium Chloride; D013395:Sucrose; D005092:Exonucleases; D005095:Exoribonucleases; C023871:nuclease H