Induction of type C virions from normal rat kidney cells by 2-deoxy-D-glucose.
The sugar 2-deoxy-D-glucose (2-DG) induced the release of type C virions from an established line of normal rat kidney (NRK) cells. Within 20 h after the addition of 5 mg of 2-DG per ml to exponentially growing NRK clutures, more than 80% of the cells expressed the mammalian type C virus interspecies-specific antigen (p30) as determined by indirect cytoplasmic immunofluorescence. Maximal virion release occurred 1 to 2 days after 2-DG was added for 24 h to the growth medium although a low level of virion production was detected as early as 2.5 h after 2-DG treatment. Studies with inhibitors of RNA synthesis indicated a requirement for de novo RNA synthesis after the addition of 2-DG. Sensitivity of NRK cells to type C virion induction was limited to a relatively short period of in vitro growth and preceded spontaneous virion release by 8 to 10 subculture generations. A model is presented for the sequential derepression of latent type C virus information in serially propagated NRK cells.
D000818:Animals; D000956:Antigens, Viral / Q000302:isolation & purification; D002460:Cell Line; D003609:Dactinomycin / Q000494:pharmacology; D003837:Deoxy Sugars / Q000494:pharmacology*; D003839:Deoxyadenosines / Q000494:pharmacology; D003847:Deoxyglucose / Q000494:pharmacology*; D005944:Glucosamine / Q000494:pharmacology; D007065:Idoxuridine / Q000494:pharmacology; D007668:Kidney; D011691:Puromycin / Q000494:pharmacology; D012367:RNA, Viral / Q000096:biosynthesis; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D051381:Rats; D012190:Retroviridae / Q000254:growth & development* / Q000276:immunology / Q000378:metabolism; D014779:Virus Replication
D000956:Antigens, Viral; D003837:Deoxy Sugars; D003839:Deoxyadenosines; D012367:RNA, Viral; D003609:Dactinomycin; D011691:Puromycin; D003847:Deoxyglucose; D012194:RNA-Directed DNA Polymerase; D007065:Idoxuridine; D005944:Glucosamine