title: Antigenic determinants of murein lipoprotein and its exposure at the surface of Enterobacteriaceae.
abstract: Murein lipoprotein from the outer membrane of Escherichia coli could be fixed to erythrocytes without pretreatment of the erythrocytes. Passive hemagglutination or immune hemolysis could thus be used as sensitive assays to determine antibodies against lipoprotein. In rabbit antisera prepared against whole cells of E. coli, Salmonella, Arizona, and Shigella antibodies against lipoprotein were present. The respective titers were lowest in encapsulated smooth strains and highest in rough mutants. Antisera against deep rough mutants showed even higher anti-lipoprotein titers than anti-R-lipopolysaccharide titers. Correspondingly,absorption of lipoprotein antibodies with enterobacterial strains was most pronounced with deep rough mutants and lowest with smooth strains. Lipoprotein becomes increasingly an immunogen as well as an antigen the more sugar residues are missing in the lipolysaccharide on the cell surface. In wild-type cells lipoprotein is buried in the outer membrane; its exposure in mutant cells is related to defects at the cell surface.
mesh_terms: D000818:Animals; D000937:Antigen-Antibody Reactions; D004355:Drug Stability; D004755:Enterobacteriaceae / Q000276:immunology*; D000939:Epitopes; D004912:Erythrocytes / Q000276:immunology; D006386:Hemagglutination Tests; D006461:Hemolysis; D006358:Hot Temperature; D006801:Humans; D008074:Lipoproteins / Q000276:immunology*; D009154:Mutation; D010457:Peptidoglycan / Q000276:immunology*; D011817:Rabbits / Q000276:immunology; D013045:Species Specificity; D013997:Time Factors
chemical_list: D000939:Epitopes; D008074:Lipoproteins; D010457:Peptidoglycan