[Comparative study of chicken liver xanthine dehydrogenase and bovine liver xanthine oxidase. dehydrogenase activity of xanthine oxidase (author's transl)].
A method to purify bovine liver xanthine oxidase in described, with which samples of 256-fold specific activity with respect to the initial homogenate are obtained. Bovine liver xanthine oxidase and chicken liver xanthine dehydrogenase with oxygen as electron acceptor exhibit similar profile in pKM and log V versus pH plots. With NAD+ as electron acceptor a different profile in the pKM xanthine plot is obtained for chicken liver xanthine dehydrogenase. However three inflection points at the same pH values appear in all plots. Both enzymes are irreversibly inhibited by pCMB and reversibly by N-ethylmaleimide and by iodoacetamide, with competitive and uncompetitive type inhibitions respectively. These results suggest that NAD+ alters the enzymatic action since its binding to the enzyme antecedes the binding of xanthine to the xanthine oxidase molecule, without undergoing itself any modification. 0.15 M DDT of DTE treatment of bovine liver xanthine oxidase gives to the enzyme a permanent activity with NAD+ without modifying its activity with oxygen. The enzyme thus treated produces parallel straight lines in Lineweaver-Burk plots.
D000818:Animals; D002417:Cattle; D002645:Chickens; D006863:Hydrogen-Ion Concentration; D007658:Ketone Oxidoreductases / Q000032:analysis*; D008099:Liver / Q000201:enzymology*; D008297:Male; D009243:NAD / Q000494:pharmacology; D014968:Xanthine Dehydrogenase / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000378:metabolism; D014969:Xanthine Oxidase / Q000032:analysis* / Q000037:antagonists & inhibitors / Q000378:metabolism
D009243:NAD; D014968:Xanthine Dehydrogenase; D014969:Xanthine Oxidase; D007658:Ketone Oxidoreductases
Estudio comparativo de la xantindeshidrogenasa de hígado de pollo y de la xantinoxidasa de hígado de buey. Actividad deshidrogenásica de la xantinoxidasa.