Cell line-specific cell surface components of hypotetraploid ascites mammary carcinoma cells inducing humoral reactions in the syngeneic host.
Cell-line specific cell surface components were demonstrated on cells of various hypotetraploid ascites cell lines, but not on hyperdiploid cells derived from a mammary carcinoma induced by mammary tumor virus (MTV) in syngeneic C3H/He mice. These substances caused cell-line-specific transplantation antigenicity and were identified, together with MTV-associated substances and tumor-associatied embryonic materials, as being the binding sites for tumor cell agglutinating factors found in sera from tumor-bearing and regressor animals. Particles containing the cell line-specific substances were released from these cells by hypnotic treatment. They could be purified as a single peak in a sucrose density gradient. They were, however, not dissociated from the MTV-associated substances. Absorption studies of agglutination activity were extensively used for demonstration of specificity.
D000371:Agglutination / Q000187:drug effects; D000372:Agglutination Tests; D000818:Animals; D000951:Antigens, Neoplasm; D001666:Binding Sites, Antibody; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology*; D000939:Epitopes; D005260:Female; D008325:Mammary Neoplasms, Experimental / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H; D009368:Neoplasm Transplantation; D011123:Polyploidy; D012965:Sodium Chloride / Q000494:pharmacology; D014181:Transplantation Immunology; D014185:Transplantation, Isogeneic
D000951:Antigens, Neoplasm; D000939:Epitopes; D012965:Sodium Chloride