title: Full length and discrete partial reverse transcripts of globin and chorion mRNAs.
abstract: Rabbit globin mRNA was copied by AMV reverse transcriptase in the presence of various concentrations of deoxyribonucleotides (dNTPs). The cDNAs were analyzed by electrophoresis under denaturing conditions in formamide-polyacrylamide gels. Discrete size products were detected, ranging from 65 to 650 nucleotides-that is, up to the full length of the mRNA template. Increasing the concentrations of all four dNTPs stimulated formation of full-length transcripts and made the incomplete copies less abundant. Hybridization and nuclease digestion experiments indicated that the full-size product is indeed a complete transcript of globin mRNA. Similar results were obtained with chorion mRNAs. The possible usefulness of the discrete partial transcripts is discussed.
mesh_terms: D000818:Animals; D001345:Autoradiography; D001354:Avian Leukosis Virus / Q000201:enzymology; D002499:Centrifugation, Density Gradient; D002823:Chorion; D004270:DNA, Circular / Q000032:analysis / Q000096:biosynthesis*; D004277:DNA, Single-Stranded / Q000032:analysis; D003609:Dactinomycin / Q000494:pharmacology; D003854:Deoxyribonucleotides / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005349:Fibroins; D005914:Globins; D009693:Nucleic Acid Hybridization; D009711:Nucleotides / Q000032:analysis; D010047:Ovalbumin; D010761:Phosphorus Radioisotopes; D012333:RNA, Messenger / Q000378:metabolism*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*; D011817:Rabbits; D013698:Templates, Genetic; D014158:Transcription, Genetic
chemical_list: D004270:DNA, Circular; D004277:DNA, Single-Stranded; D003854:Deoxyribonucleotides; D009711:Nucleotides; D010761:Phosphorus Radioisotopes; D012333:RNA, Messenger; D003609:Dactinomycin; D005914:Globins; D010047:Ovalbumin; D005349:Fibroins; D012194:RNA-Directed DNA Polymerase