Virion and soluble antigens of japanese encephalitis virus.
Japanese encephalitis virions contain a 58 X 10-3-molecular-weight envelope glycoprotein antigen that can be solubilized with sodium lauryl sulfate and separated from other virion structural polypeptides and viral ribonucleic acid by gel filtration chromatography. The 58 X 10-3-molecular-weight envelope protein is the major antigen responsible for cross-reactivity of the virion in complement fixation tests with other closely related arboviruses. A naturally occurring soluble complement-fixing antigen is found in Japanese encephalitis mouse brain preparations after removal of particulate antigens. After partial purification by gel filtration and isoelectric focusing, the 53 X 10-3-molecular weight soluble complement-fixing antigen is more type specific than the Japanese encephalitis envelope antigen in complement fixation tests. Further, the Japanese encephalitis soluble complement-fixing antigen is stable to treatment with sodium lauryl sulfate and 2-mercaptoethanol, whereas virion complement-fixing antigens are unstable after this treatment.
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D001202:Ascitic Fluid / Q000276:immunology; D001921:Brain; D003168:Complement Fixation Tests; D003429:Cross Reactions; D046508:Culture Techniques; D004591:Electrophoresis, Polyacrylamide Gel; D004664:Encephalitis Virus, Japanese / Q000276:immunology*; D000939:Epitopes; D005944:Glucosamine / Q000378:metabolism; D006386:Hemagglutination Tests; D007114:Immunization; D007525:Isoelectric Focusing; D051379:Mice; D010455:Peptides / Q000032:analysis; D012995:Solubility; D014316:Tritium; D010948:Viral Plaque Assay; D014776:Virus Cultivation
D000596:Amino Acids; D000956:Antigens, Viral; D000939:Epitopes; D010455:Peptides; D014316:Tritium; D005944:Glucosamine