Immunosuppressive factor(s) extracted from lymphoid cells of nonresponder mice primed with L-glutamic acid60-L-alanine30-L-tyrosine10 (GAT).
The synthetic terpolymer of L-glutamic acid60-L-alanine30-L-tyrosine10 (GAT) not only fails to elicit a GAT-specific antibody response in nonresponder mice, but also prior injection of GAT specifically decreases the ability of nonresponder mice to develop a GAT-specific antibody response to a subsequent challenge with GAT-MBSA. This inhibition is mediated by GAT-specific suppressor T cells. Further, a suppressive factor can be extracted from lymphoid cells of GAT-primed nonresponder mice that inhibits the development of primary GAT-specific antibody responses to GAT-MBSA and to GAT-PRBC- by normal syngeneic mice. The suppressive activity is dose-dependent and absorbed by GAT-Sepharose, but not by BSA-Sepharose. The suppressive activity elutes from a G-100 Sephadex column in the same fraction as ovalbumin, suggesting its m.w. is approximately 45,000 daltons.
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D002474:Cell-Free System; D000939:Epitopes; D006462:Hemolytic Plaque Technique; D007166:Immunosuppressive Agents / Q000032:analysis / Q000302:isolation & purification*; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008805:Mice, Inbred A; D008811:Mice, Inbred DBA; D008970:Molecular Weight; D009842:Oligopeptides / Q000494:pharmacology*; D010455:Peptides / Q000276:immunology
D000939:Epitopes; D007166:Immunosuppressive Agents; D009842:Oligopeptides; D010455:Peptides