Polyglutaraldehyde: a new reagent for coupling proteins to microspheres and for labeling cell-surface receptions. II. Simplified labeling method by means of non-magnetic and magnetic polyglutaraldehyde microspheres.
Procedures were developed for the synthesis of a new immunoreagent in form of polyglutaraldehyde (PGL) microspheres in sizes ranging from about 50 nm to 1.5 micron. Addition of fluorochromes during synthesis yielded microspheres of high fluorescence intensity. By carrying out the polymerization of glutaraldehyde in presence of iron oxide, magnetic PGL microspheres were produced. Antibody conjugates obtained by interaction of PGL microspheres with immunoglobulins were used to label human red blood cells (RBC) and lymphocytes. A simple method for the separation of magnetically labeled human RBC from unlabeled cells was demonstrated.
D000447:Aldehydes; D000818:Animals; D002462:Cell Membrane / Q000276:immunology; D004912:Erythrocytes / Q000378:metabolism; D005976:Glutaral; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000378:metabolism; D007158:Immunologic Techniques / Q000379:methods; D008280:Magnetics; D008863:Microspheres; D011108:Polymers; D011485:Protein Binding; D011817:Rabbits
D000447:Aldehydes; D007074:Immunoglobulin G; D011108:Polymers; D005976:Glutaral