Effect of pentobarbital anesthesia on serotonin metabolism in hypoxic rat brain.
Two hours of pentobarbital anesthesia resulted in marked elevation of serotonin (5-HT) level and decreased accumulation of 5-hydroxyindolacetic acid (5-HIAA) after probenecid, in specific parts of rat brain. In hypoxia, either no change (cortex, hypothalamus) or an increase (striatum, pons with medulla) in 5-HT content was observed. Concomitantly, the decrease of 5-HIAA level and its accumulation after transport inhibition occured. Pentobarbital pretreatment of hypoxic rats resulted in further diminution of 5-HT degradation after probenecid, having synergistic character in pons with medulla, which points to the specific sensitivity of serotonergic system in the latter area. Hypoxia-induced decrease of 5-HIAA level was prevented by pentobarbital pretreatment. The possible participation of 5-HIAA efflux inhibition in this phenomenon is discussed.
D000758:Anesthesia; D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects*; D002245:Carbon Dioxide / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006897:Hydroxyindoleacetic Acid / Q000378:metabolism; D000860:Hypoxia / Q000378:metabolism*; D008297:Male; D010101:Oxygen Consumption; D010293:Pargyline / Q000494:pharmacology; D010424:Pentobarbital / Q000494:pharmacology*; D011339:Probenecid / Q000494:pharmacology; D051381:Rats; D012701:Serotonin / Q000378:metabolism*
D002245:Carbon Dioxide; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D010293:Pargyline; D010424:Pentobarbital; D011339:Probenecid