Retardation of development including immunogenic expression of histocompatibility antigens in mice--by postnatal administration of antiandrogenic steroid.
A specific antiandrogenic steroid cyproterone acetate was administered daily to mice of three different inbred strains starting from the day of birth until the age of 30 days. The total dose per mouse was 17.2 mg. This treatment resulted in developmental retardation which was manifested in a number of ways: at the age of 30 days, the weight of the body was well as spleen, testes and particularly thymus was significantly reduced; histologically, the normal proportion of the red and white pulp in the spleen was changes; spermatogenesis (but not oogenesis) was markedly retarded corresponding to the age of 12-15 days in normal males; also skin displayed a persisting immaturity as reflected by an abundance of mast cells. Minor signs of toxic changes were seen in the liver. Skin grafts from CA-pretreated donors had a subnormal immunogenicity; when transplanted across the MSA-barrier, they survived significantly longer than control grafts and about 23% took. Significantly prolonged survival was also observed with H-3 incompatible skin grafts from CA-pretreated donors, particularly from male donors. Across the barrier dicated, but did not reach a level of significance. The present study extends our previous observations concerning the androgen dependence of a normal immunogenic expression of H-antigens. The antiandrogenic effect of CA is comparable to the more complex effect of neonatal orchiectomy in terms of the subnormal immunogeneity of MSA-incompatible skin grafts from 30-day-old males which seems to be arrated at a stage typical for 1-2-day-old normal males.
D000726:Androgen Antagonists; D000818:Animals; D000831:Animals, Newborn; D000917:Antibody Formation / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D003534:Cyproterone / Q000494:pharmacology*; D000939:Epitopes; D005260:Female; D006087:Graft vs Host Reaction / Q000187:drug effects; D006128:Growth / Q000187:drug effects*; D006649:Histocompatibility Antigens; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL / Q000276:immunology; D009929:Organ Size; D016038:Skin Transplantation; D013091:Spermatogenesis / Q000187:drug effects; D014184:Transplantation, Homologous
D000726:Androgen Antagonists; D000939:Epitopes; D006649:Histocompatibility Antigens; D003534:Cyproterone