Factors affecting protein synthesis during biotin deficiency in Aspergillus nidulans.
Biotin deficiency in Aspergillus nidulans resulted in a 70% increase of the protein content and increased levels of free and bound aspartate, glutamate, serine, leucine and methionine. Likewise, the activities of NADP+ glutamate dehydrogenase, NAD+ gluatmate dehydrogenase, aspartate aminotransferase and alanine aminotransferase were significantly increased. The total RNA content increased while the DNA content was unaffected. The rRNA/tRNA ratio remained higher in biotin-deficient cells. Supplementation of glutamate, aspartate, serine, leucine and methionine to the culture medium raised the rRNA/tRNA ratio, and the difference observed in the qualitative and the quantitative patterns of protein and dry cell mass between normal and biotin-deficient cultures was abolished.
D000410:Alanine Transaminase / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D001219:Aspartate Aminotransferases / Q000378:metabolism; D001233:Aspergillus nidulans / Q000378:metabolism*; D001710:Biotin / Q000378:metabolism*; D002474:Cell-Free System; D004271:DNA, Fungal / Q000096:biosynthesis; D005656:Fungal Proteins / Q000096:biosynthesis*; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism; D012331:RNA, Fungal / Q000096:biosynthesis
D000596:Amino Acids; D004271:DNA, Fungal; D005656:Fungal Proteins; D012331:RNA, Fungal; D001710:Biotin; D005969:Glutamate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D005974:Glutamate-Ammonia Ligase