title: A hybrid technique for staining meiotic nuclei of Basidiomycetes.
abstract: A hybrid nuclear staining procedure used on the basidiomycete Schizophyllum commune produced squash preparations of sufficient density for effective brightfield photomicrography.
  
  
  METHODS
  Tissure is fixed in Newcomer's solution, rehydrated in a graded ethanol series, rinsed, hydrolyzed in 1 N HC1 at 60 C, washed, mordanted in 4% iron alum, rinsed again, and stained with aceto-iron-hematoxylin prior to mounting and squashing.
mesh_terms: D000363:Agaricales / Q000648:ultrastructure*; D002467:Cell Nucleus / Q000648:ultrastructure*; D002875:Chromosomes / Q000648:ultrastructure; D006416:Hematoxylin; D008540:Meiosis; D012567:Schizophyllum / Q000648:ultrastructure*; D013194:Staining and Labeling
chemical_list: D006416:Hematoxylin