Colloidal gold probes--a further evaluation.
Colloidal gold provides an immuno- and cytochemical marker suitable both for TEM and SEM. Various parameters involved in the preparation and stability of gold markers of various sizes and of probes have been reviewed and modifications in methodology suggested based on a study of factors affecting the performance of the gold marker system. In particular, dependence of protein adsorption on pH conditions correlating with protein pI is further confirmed. Presence of bioactive protein in gold probes is demonstrated and quantitated by radioassay (125I) and agglutination procedures.
D002460:Cell Line; D002462:Cell Membrane / Q000648:ultrastructure; D003102:Colloids; D003208:Concanavalin A / Q000378:metabolism; D004910:Erythrocyte Membrane / Q000648:ultrastructure; D006046:Gold; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008565:Membrane Proteins; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D011506:Proteins; D013329:Structure-Activity Relationship
D003102:Colloids; D008565:Membrane Proteins; D011506:Proteins; D003208:Concanavalin A; D006046:Gold