Properties of bispecific rosette-forming cells. II. --Rosette formation by "educated" T-lymphocytes.
Normal thymus cells transferred into thymectomized and lethally irradiated syngeneic mice responded to stimulation with sheep erythrocytes by rosette formation with these erythrocytes. This response reached a peak on the 7th day and was not associated with any production of circulating antibodies. Rosettes produced by "educated" T-cells were inhibited by anti-theta serum as well as by anti-mouse Fab serum. Simultaneous stimulation of transferred thymocytes with sheep and pigeon erythrocytes provoked the appearance of a minority of cells simultaneously binding both types of erythrocytes. Depletion of B cells contaminating normal thymocyte populatins after passage through anti-mouse Ig coated columns before their transfer in thymectomized and irradiated recipients did not prevent the appearance of simple and double RFC. Moreover, when normal thymocytes or T-cells "educated" by allogeneic stimulation were incubated at 4 degrees C with anti-SRBC and anti-PRBC mouse sera a subsequent incubation at 37 degress of resulted in the dissociation of most passive rosettes formed at 4 degrees. Conversely similar incubation at 37 degrees of rosettes formed by actively immunized cells resulted in capping of about 50% simple and double rosettes. This redistribution of membrane receptors is proposed as a routine test for distinguishing active from passive rosettes.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000378:metabolism; D000961:Antilymphocyte Serum / Q000302:isolation & purification; D002449:Cell Aggregation; D000939:Epitopes; D004912:Erythrocytes / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D007106:Immune Sera / Q000302:isolation & purification; D007111:Immunity, Cellular; D007140:Immunoglobulin Fab Fragments / Q000302:isolation & purification; D007165:Immunosuppression Therapy; D051379:Mice; D008808:Mice, Inbred CBA; D011817:Rabbits; D011828:Radiation Chimera; D011947:Receptors, Antigen, B-Cell; D011955:Receptors, Drug; D013601:T-Lymphocytes / Q000276:immunology*; D013950:Thymus Gland / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D000961:Antilymphocyte Serum; D000939:Epitopes; D007106:Immune Sera; D007140:Immunoglobulin Fab Fragments; D011947:Receptors, Antigen, B-Cell; D011955:Receptors, Drug