[Immobilization of aspergillus oryzae aminopeptidase on organic and inorganic carriers].
The process of Asp. oryzae aminopeptidase immobilization on organic (AE-cellulose, sepharose 4B, Sephadex G-200) and inorganic (SCh-2, SCh-3 sylochromes and KCK N 1 silicagel) carriers was studied. Aminopeptidase immobilized on Sephadex G-200 contains the largest amount of protein (80 mg per 1 g of carrier) and is the most active of all other preparations. The immobilized preparations retain the temperature optimum, like the soluble form, at 60 degrees C, except the preparation immobilized on AE-cellulose (50 degrees C). At temperatures of 70, 80 degrees C the obtained preparations are more thermostable than the initial enzyme. The pH-stability zone for the preparations immobilized by means of sylochrome-3 and Sephadex G-200 is wider to some extent as compared to the soluble enzyme. When studying the substrate specificity it is established that leucyl-glycyl-glycine is the best substrate for both the soluble form and the immobilized preparations. Multiple application of the immobilized aminopeptidase is shown to be possible.
D000626:Aminopeptidases / Q000378:metabolism*; D001230:Aspergillus / Q000201:enzymology*; D001236:Aspergillus oryzae / Q000201:enzymology*; D002482:Cellulose; D004355:Drug Stability; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008722:Methods; D012685:Sepharose; D012822:Silicon Dioxide
D004800:Enzymes, Immobilized; D012822:Silicon Dioxide; D002482:Cellulose; D012685:Sepharose; D000626:Aminopeptidases
Immobilizatsiia aminopeptidazy A spergillus oryzae na organicheskikh i neorganicheskikh nositeliakh.