Regulation of dopamine synthesis in striatal synaptosomes during depolarization.
Incubation of striatal synaptosomes under depolarizing conditions (veratridine or KCl) increased the release of dopamine (DA) and produced a small increase in DA synthesis. This was not accompanied by an activation of either soluble or membrane-bound tyrosine hydroxylase, but was associated with a large depletion of synaptosomal DA. Thus, depolarization may increase DA synthesis by reducing the inhibition of tyrosine hydroxylase by end-product.
D000818:Animals; D002458:Cell Fractionation; D002794:Choline / Q000494:pharmacology; D003342:Corpus Striatum / Q000187:drug effects / Q000201:enzymology*; D004298:Dopamine / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D008297:Male; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats; D009435:Synaptic Transmission / Q000187:drug effects; D013574:Synaptosomes / Q000187:drug effects / Q000201:enzymology*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism; D014701:Veratridine / Q000494:pharmacology
D011189:Potassium Chloride; D014701:Veratridine; D014446:Tyrosine 3-Monooxygenase; D002794:Choline; D004298:Dopamine