Immunochemical relationship between alpha-amylases of rat liver, serum, pancreas and parotid gland.
1. Rabbit antisera to purified rat parotid alpha-amylase were prepared. 2. The relationships between rat parotid-gland, pancreatic, serum and liver amylase were investigated by using the antisera in immunodiffusion, immunoelectrophoresis and immunoinhibition experiments. 3. Serum and liver amylase were identical, and very similar to parotid-gland amylase; pancreatic amylase was, however, quite distinct, and showed only some of the determinants present on parotid-gland amylase. 4. The data strengthen the suggestion that the liver is the main source of serum amylase.
D000681:Amylases / Q000037:antagonists & inhibitors / Q000097:blood / Q000276:immunology*; D000818:Animals; D000939:Epitopes; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D008099:Liver / Q000201:enzymology*; D008297:Male; D009928:Organ Specificity; D010179:Pancreas / Q000201:enzymology*; D010306:Parotid Gland / Q000201:enzymology*; D011817:Rabbits; D051381:Rats
D000939:Epitopes; D000681:Amylases