Antibodies to fentanyl.
Immunization of rabbits with carboxyfentanyl-bovine gamma-globulin conjugate induced antibodies of high titers capable of binding with fentanyl.  The antibodies exhibited high average association constants (approximately 1 times 10-7 liter/mol) and were highly specific to fentanyl since no cross-reactivity was observed for a variety of test compounds including other opiates and analgesics.  Serological cross-reactivity was observed only for chemicals representing various portions of the fentanyl molecule.  These high-affinity, highly selective antibodies have been successfully employed in a radioimmunoassay for fentanyl.  Serum concentrations of fentanyl, after administration of 100 and 21 mug/kg/ i.v. in dogs, were determined by this radioimmunoassay.  The specificity of these antibodies as compared with the specificity of the pharmacological (opiate) receptor is also discussed.
D000818:Animals; D000917:Antibody Formation; D000936:Antigen-Antibody Complex; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D001794:Blood Pressure; D002417:Cattle; D003429:Cross Reactions; D003956:Dialysis; D005283:Fentanyl / Q000097:blood / Q000276:immunology*; D007106:Immune Sera; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay; D013997:Time Factors; D005719:gamma-Globulins
D000936:Antigen-Antibody Complex; D007106:Immune Sera; D005719:gamma-Globulins; D005283:Fentanyl