Specificity of antigenic fractions of tuberculin.
Purified protein derivative from Mycobacterium bovis was separated into 6 fractions by electrophoresis at 1500 V/40 mA in pH 4.2 acetic acid-pyridine buffer. Further purification of one of the fractions on Sephadex G 25 column and by acid hydrolysis yielded antigen "PS" eliciting tuberculin reaction only in animals vaccinated with M. bovis BCG but not in those sensitized with M. avium and some fast-growing mycobacteria. The specificity of antigen "PS" was confirmed in vitro: the antigen induced blastic transformation only in lymphocytes of guinea pigs sensitized with M. bovis BCG.
D000818:Animals; D002417:Cattle; D005591:Chemical Fractionation; D000939:Epitopes; D006168:Guinea Pigs / Q000276:immunology; D008213:Lymphocyte Activation; D009162:Mycobacterium avium / Q000276:immunology; D009163:Mycobacterium bovis / Q000276:immunology*; D014373:Tuberculin / Q000032:analysis*; D014374:Tuberculin Test; D014380:Tuberculosis, Bovine / Q000276:immunology
D000939:Epitopes; D014373:Tuberculin