A comparison of two methods of preparing cells or nuclei for high-resolution microspectrophotometry.
Suspensions of isolated mouse thymus cells were subjected to two preparative methods: either they were dropped through several mm of 9:1 v/v ethanol-acetic acid fixative, allowed to stand for 1hr and then processed for staining; or they were fixed, passed through a graded ethanol series to 70% ethanol, centrifuged on to slides in a modified Shandon cytocentrifuge and then carried wet into the staining procedure. All preparations were stained by the Feulgen reaction and evaluated by high-resolution microspectrophotometry. While the two preparative procedures yielded similar results, there appeared to be less variability in the data obtained from the centrifuged cell populations.
D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure*; D005069:Evaluation Studies as Topic; D006651:Histocytochemistry; D008722:Methods; D051379:Mice; D013053:Spectrophotometry; D013194:Staining and Labeling; D013950:Thymus Gland / Q000166:cytology*