title: Migration of ribosomes along the axons of the chick visual pathway.
abstract: The axoplasmic migration of ribosomes has been detected in the visual system of the chick. Monocular injection of radioactive uridine or an amino acid mixture was followed by sedimentation analysis in sucrose or cesium sulfate density gradients, of ribosomes prepared from the retinae of injected eyes and the left and right optic lobes. By this means both RNA and protein components of ribosomes were found to migrate from the retina to the innervated contralateral optic lobe. Following denervation of the distal nerve segment by eye removal, the stability of the transported RNA was reduced, suggesting its presynaptic location. The transport of RNA was not significantly imparied by intraocular injection of inhibitors of informational RNA or mitochondrial RNA synthesis prior to injection of radioactive uridine but was depressed by a low dose of actinomycin D.
mesh_terms: D000546:Amanitins / Q000494:pharmacology; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001370:Axonal Transport; D001369:Axons / Q000502:physiology*; D002499:Centrifugation, Density Gradient; D002645:Chickens; D003609:Dactinomycin / Q000494:pharmacology; D004996:Ethidium / Q000494:pharmacology; D014176:Protein Biosynthesis; D012269:Ribosomal Proteins / Q000494:pharmacology; D012270:Ribosomes / Q000187:drug effects / Q000502:physiology* / Q000648:ultrastructure; D012293:Rifampin / Q000494:pharmacology; D013997:Time Factors; D014158:Transcription, Genetic; D014529:Uridine / Q000378:metabolism; D014795:Visual Pathways / Q000502:physiology*
chemical_list: D000546:Amanitins; D000596:Amino Acids; D012269:Ribosomal Proteins; D003609:Dactinomycin; D004996:Ethidium; D012293:Rifampin; D014529:Uridine