Sepharose-bound erythropoientin. Studies with anti-erythropoietin.
Anti-erythropoietin antibodies (anti-ESF) were determined by the capacity of the immunoglobulins to neutralize the biological activity of erythropoietin (ESF). Affinity chromatographic methods were used to initiate the purification of anti-ESF by use of ESF covalently linked to Sepharose. It was observed that anti-ESF bound the ESF-Sepharose. In order to remove the nonspecific immunoglobulins, the anti-ESF preparation was subjected to further chromatography on affinity colums where either serum or urinary proteins from a polycythemic patient were covalently linked to Sepharose. It was demonstrated the affinity chromatography could be used as a method isolate anti-ESF.
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D001666:Binding Sites, Antibody; D002846:Chromatography, Affinity; D003488:Cyanogen Bromide; D000939:Epitopes; D004921:Erythropoietin / Q000276:immunology*; D006801:Humans; D007114:Immunization; D007158:Immunologic Techniques; D009500:Neutralization Tests; D011087:Polycythemia Vera / Q000097:blood / Q000276:immunology; D011817:Rabbits / Q000276:immunology; D012685:Sepharose
D000906:Antibodies; D000939:Epitopes; D004921:Erythropoietin; D012685:Sepharose; D003488:Cyanogen Bromide