Recognition by pregnancy serums of non-HL-A alloantigens selectively expressed on B lymphocytes.
A group of alloantibodies are found in pregnancy sera which react with antigens present on B lymphocytes and monocytes but are not detectable on the vast majority of unstimulated T cells. This specificity distinguishes them from HL-A antibodies which react with both cell types. They were readily recognized through indirect fluorescent antibody analysis by employing the combination of B-cell lymphoid lines and normal peripheral blood T cells. Different sera gave a variety of patterns of reactivity with a panel of 11 lymphoid lines. Similar differential patterns were also observed with normal B cells from different individuals particularly after concentrating the B cells. The antibodies were also cytotoxic to B cells and this procedure gave parallel results to the fluorescence method. The pattern of reactions obtained indicated a very heterogeneous system similar to that for HL-A. Special study of certain of the sera provided evidence that the lymphocyte-defined determinants of the mixed lymphocyte reaction system were involved. For convenience the term HL-B has been employed for these antigens.
D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000276:immunology*; D003601:Cytotoxicity Tests, Immunologic; D000939:Epitopes; D005260:Female; D005455:Fluorescent Antibody Technique; D006650:Histocompatibility Testing; D006801:Humans; D007104:Immune Adherence Reaction; D007106:Immune Sera; D007074:Immunoglobulin G; D007519:Isoantigens; D008213:Lymphocyte Activation; D008856:Microscopy, Fluorescence; D008858:Microscopy, Phase-Contrast; D009000:Monocytes / Q000276:immunology; D011247:Pregnancy; D013601:T-Lymphocytes / Q000276:immunology
D000939:Epitopes; D007106:Immune Sera; D007074:Immunoglobulin G; D007519:Isoantigens