Characterization of DNA polymerase and RNA associated with A-type particles from murine myeloma cells.
The RNA-dependent DNA polymerase present in intracisternal A-type particles from mouse myeloma tumor cells has been studied. This polymerase can use either endogenous A particle RNA or an exogenous synthetic polynucleotide [poly (rA)] as a template. The DNA reaction product is small (4S-10S) and over 90% of it hybridizes to A particle RNA, whereas up to 50% of it hybridizes to murine sarcoma-leukemia virus RNAs. The RNA isolated from purified A particles is generally of low molecular weight (5S-15S) but contains small amount of 70S and 35S components. These results suggest that A-type particles may be related to C-type oncornaviruses.
D000818:Animals; D002458:Cell Fractionation; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D004279:DNA, Viral / Q000096:biosynthesis; D003609:Dactinomycin / Q000494:pharmacology; D017988:Gammaretrovirus / Q000032:analysis / Q000201:enzymology; D051379:Mice; D008807:Mice, Inbred BALB C; D008854:Microscopy, Electron; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D010954:Plasmacytoma; D011092:Polyethylene Glycols / Q000494:pharmacology; D011119:Polynucleotides / Q000378:metabolism; D012367:RNA, Viral / Q000032:analysis / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D013698:Templates, Genetic; D013942:Thymine Nucleotides / Q000378:metabolism; D014316:Tritium
D004279:DNA, Viral; D011119:Polynucleotides; D012367:RNA, Viral; D013942:Thymine Nucleotides; D014316:Tritium; D003609:Dactinomycin; D011092:Polyethylene Glycols; D012194:RNA-Directed DNA Polymerase