Soil ameba infection. Specific indirect immunoenzymatic (peroxidase) staining of formalin-fixed paraffin sections.
This report describes the use of the immunoenzymatic (peroxidase) method to identify the species and to stain distinctively the amebas in formalin-fixed paraffin-mounted sections. This permits the use of hematoxylin and eosin counterstaining. The method, now well developed by others for many purposes, is an alternative to immunofluorescence and seems to offer a number of advantages and a lesser number of disadvantages.
D000562:Amebiasis / Q000175:diagnosis* / Q000201:enzymology / Q000276:immunology; D000656:Amoeba / Q000276:immunology*; D000818:Animals; D000906:Antibodies; D001560:Benzidines / Q000276:immunology; D005916:Globulins; D006041:Goats / Q000276:immunology; D000882:Haplorhini; D006249:Hartmannella / Q000276:immunology; D006652:Histological Techniques; D006801:Humans; D007106:Immune Sera / Q000032:analysis; D007158:Immunologic Techniques; D051379:Mice; D010544:Peroxidases; D011817:Rabbits / Q000276:immunology; D012965:Sodium Chloride / Q000276:immunology; D012988:Soil Microbiology; D013194:Staining and Labeling
D000906:Antibodies; D001560:Benzidines; D005916:Globulins; D007106:Immune Sera; D012965:Sodium Chloride; D010544:Peroxidases