Human pituitary growth hormone: restoration of full biological activity by noncovalent interaction of two fragments of the hormone.
The NH2-terminal 134 amino-acid fragment of the reduced-carbamidomethylated human somatropin molecule is found to react noncovalently with the COOH-terminal 51 amino-acid fragment in solutions of pH 8.4 at 2 degrees to restore full biological activity as evidenced by the rat tibia and pigeon crop-sac assays. In addition, circular dichroism spectra of the recombinant show the conformation to be completely repaired in comparison with that of the native hormone.
D001681:Biological Assay; D002942:Circular Dichroism; D000939:Epitopes; D005341:Fibrinolysin; D013006:Growth Hormone / Q000032:analysis / Q000276:immunology / Q000502:physiology*; D006801:Humans; D011388:Prolactin / Q000502:physiology; D011487:Protein Conformation; D013329:Structure-Activity Relationship
D000939:Epitopes; D011388:Prolactin; D013006:Growth Hormone; D005341:Fibrinolysin