Removal of inhibitors against RNA-directed DNA polymerase activity in human milk.
Milk from a number of species (e.g., man, mouse, rat, dog, and cow) contains inhibitors of the RNA-directed DNA polymerase. When attempts are made to isolate virions from the milk, part of the inhibitors follow the virions in the purification. The amount of inhibitors varies in different milk samples. These inhibitors can probably account for the large discrepancies reported in studies of the presence of oncornaviruses in human milk. Phosphatases bound to subcellular particles or fragments seem to be the most important inhibitors in the milk interfering with the RNA-directed DNA polymerase assay. It is shown that the inhibitory enzymes can be completely removed by sedimentation of the milk through a Metrizamide gradient.
D000818:Animals; D002417:Cattle; D004285:Dogs; D006801:Humans; D008324:Mammary Tumor Virus, Mouse / Q000302:isolation & purification; D008793:Metrizamide; D051379:Mice; D008892:Milk / Q000032:analysis / Q000201:enzymology; D008895:Milk, Human / Q000201:enzymology* / Q000382:microbiology; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis; D012194:RNA-Directed DNA Polymerase / Q000302:isolation & purification; D018894:Reverse Transcriptase Inhibitors
D018894:Reverse Transcriptase Inhibitors; D012194:RNA-Directed DNA Polymerase; D010744:Phosphoric Monoester Hydrolases; D008793:Metrizamide