Amino acid sequence studies of horseradish peroxidase. Amino and carboxyl termini, cyanogen bromide and tryptic fragments, the complete sequence, and some structural characteristics of horseradish peroxidase C.
Horseradish peroxidase C dominates quantitatively among the isoperoxidases of horseradish root and has an isoelectric point close to 9. It consists of a hemin prosthetic group, 2 Ca2+ and 308 amino acid residues, including 4 disulfide bridges, in a single polypeptide chain that carries 8 neutral carbohydrate side-chains. The molecular weight of the polypeptide chain is 33890. Assuming an average carbohydrate composition of (GlcNAc)2, Man3, Fuc, Xyl for each carbohydrate chain, the molecular weight of native horseradish peroxidase C is close to 44 000. Cyanogen bromide fragments of reduced and carboxymethylated apo-peroxidase were purified by a combination of gel filtration and isoelectric focusing in urea, and cystine-containing tryptic fragments of apo-peroxidase were purified by gel filtration followed by disulfide cleavage and rechromatography at the initial conditions. The present paper discusses (a) isoelectric points and charge distribution within the native protein, the apoprotein and the cyanogen bromide fragments, (b) a buried pyrrolidonecarboxylyl amino terminus, (c) heterogeneity at the carboxyl terminus, and (d) a possible domain structure, likely from partial tryptic digestion.
D000595:Amino Acid Sequence; D002268:Carboxypeptidases; D002850:Chromatography, Gel; D003488:Cyanogen Bromide; D006735:Horseradish Peroxidase; D006868:Hydrolysis; D010455:Peptides / Q000032:analysis / Q000302:isolation & purification; D010544:Peroxidases; D011751:Pyroglutamyl-Peptidase I; D014357:Trypsin
D010455:Peptides; D006735:Horseradish Peroxidase; D010544:Peroxidases; D002268:Carboxypeptidases; D011751:Pyroglutamyl-Peptidase I; D014357:Trypsin; D003488:Cyanogen Bromide