Simian virus to (SV40) tumor-specific proteins in nucleus and plasma membrane of HeLa cells infected by adenovirus 2-SV40 hybrid virus Ad2+ND2.
HeLa cells infected with the nondefective adenovirus 2-SV40 hybrid virus (Ad2+ND2) have previously been shown to synthesize two SV40-specific proteins with molecular weights of 56,000 (56 K protein) and 42,000 (42 K protein). The present study demonstrates that these proteins are immunoprecipitable with serum from hamsters bearing SV40 tumors. By peptide analysis, it is shown that the 56 K and 42 K proteins share common amino acid sequences. Both proteins are metabolically stable in nuclei and plasma membranes but unstable in the cytoplasm, as shown by pulse-chase experiments and cell fractionation studies.
D000256:Adenoviridae / Q000378:metabolism*; D000912:Antibodies, Neoplasm / Q000032:analysis; D002462:Cell Membrane / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D000939:Epitopes; D006367:HeLa Cells; D006824:Hybridization, Genetic; D010455:Peptides / Q000032:analysis; D013539:Simian virus 40 / Q000378:metabolism*; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology
D000912:Antibodies, Neoplasm; D000939:Epitopes; D010455:Peptides; D014764:Viral Proteins