Further investigations on the affinity chromatography of unlabeled and labeled cortisol-binding immunoglobulin.
A specific method for the isolation of cortisol-binding immunoglobulin from rabbit antiserum by affinity chromatography on sepharose-coupled haptene (sepharose-cortisol) is described. This isolation technique has the advantage that no nonspecific gamma-globulin is present in the eluate of the column; previous absorption of anti-BSA-globulin can be omitted. The nature of the isolated immunoglobulin is determined by immuno-electrophoresis. The same isolation procedure can be used for the purification of radioactive antibody for radioimmunometric assay after labeling the immunoglobulin with iodine-125.
D000818:Animals; D001665:Binding Sites; D002846:Chromatography, Affinity; D006801:Humans; D006854:Hydrocortisone / Q000097:blood*; D007122:Immunoelectrophoresis; D007136:Immunoglobulins / Q000302:isolation & purification*; D011485:Protein Binding; D011817:Rabbits / Q000276:immunology; D013056:Spectrophotometry, Ultraviolet; D005719:gamma-Globulins / Q000302:isolation & purification*
D007136:Immunoglobulins; D005719:gamma-Globulins; D006854:Hydrocortisone