[Conditions of periodic oxidation in the determination of the fluorescent PAS reaction].
The effect of temperature, pH, and concentration of potassium periodate solution on the intensity of fluorescence PAS-reaction was studied. The reaction was shown to be most specific in respect to glycogen at the temperature of oxidizer 4 degrees C (optimal time of oxidation being 10-12 hr). Similar results were also obtained at 20 degrees (optimal time of oxidation being 1.5 hr). Under these conditions the curve of dependence of the reaction intensity on the time of auramine-SO2 treatment double-stepped character which can be due to the presence of two glycogen fractions in hepatic cells. The fluorescence PAS-reaction intensity varied in significantly when the periodate concentration changed from 0.8 to 1.2% (from 0.2 to 0.8%) it gradually increased and pH -from 2.0 to 6.0.
D000818:Animals; D001576:Benzophenoneidum; D005453:Fluorescence; D006863:Hydrogen-Ion Concentration; D008112:Liver Glycogen / Q000032:analysis*; D008722:Methods; D010084:Oxidation-Reduction; D010504:Periodic Acid; D051381:Rats; D013194:Staining and Labeling; D013696:Temperature; D013997:Time Factors
D008112:Liver Glycogen; D010504:Periodic Acid; D001576:Benzophenoneidum
Usloviia periodatnogo okisleniia pri provedenii fluorestsentnoĭ PAS-reaktsii