Partial purification of a serum factor that causes necrosis of tumors.
Tumor necrosis can be induced in transplanted mouse methylcholanthrene-induced sarcoma by a tumor necrosis factor in the serum of mice infected with bacillus Calmette-Guérin and given bacterial endotoxin. Sera from normal mice, endotoxin-treated mice, and mice infected with bacillus Calmette-Guérin do not contain this factor. A 20- to 30-fold purification of the serum factor has been achieved by (NH4)2SO4 fractionation, Sephadex G-100 and G-200 gel filtration, and preparative polyacrylamide electrophoresis. Tumor necrosis factor is not bacterial endotoxin. It migrates with alpha-globulins, is made up of at least four subunits, and has a molecular weight of about 150,000. The active factor is a glycoprotein that contains sialic acid and galactosamine.
D000510:Alpha-Globulins / Q000302:isolation & purification; D000818:Animals; D001500:BCG Vaccine; D001798:Blood Proteins / Q000302:isolation & purification*; D004731:Endotoxins; D005354:Fibrosarcoma / Q000473:pathology / Q000628:therapy; D005688:Galactosamine / Q000097:blood; D006023:Glycoproteins / Q000097:blood*; D008748:Methylcholanthrene; D051379:Mice; D008970:Molecular Weight; D009163:Mycobacterium bovis; D009336:Necrosis; D009374:Neoplasms, Experimental / Q000473:pathology / Q000628:therapy*; D012794:Sialic Acids / Q000097:blood
D000510:Alpha-Globulins; D001500:BCG Vaccine; D001798:Blood Proteins; D004731:Endotoxins; D006023:Glycoproteins; D012794:Sialic Acids; D008748:Methylcholanthrene; D005688:Galactosamine