Hexokinase of the adult dog heartworm, Dirofilaria immitis.
1. Dirofilaria immitis hexokinase was relatively heat stable and had a pH optimum range between 7.8 and 8.2. 2. Mean Vmax was 0.40 +/- 0.10 (S.D.) mumole/min/100 mg of worm. 3. Mean Km values were 0.32 mM for glucose, 0.86 mM for fructose and 0.39 mM for ATP. 4. Glucose-6-phosphate was not a strong product inhibitor. 5. Starch gel electrophoresis demonstrated at least three isozymes.
D000818:Animals; D004183:Dirofilaria immitis / Q000201:enzymology*; D004285:Dogs; D004592:Electrophoresis, Starch Gel; D004795:Enzyme Stability; D005370:Filarioidea / Q000201:enzymology*; D005958:Glucosephosphates / Q000494:pharmacology; D006593:Hexokinase / Q000032:analysis* / Q000037:antagonists & inhibitors; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000502:physiology; D013379:Substrate Specificity; D013696:Temperature
D005958:Glucosephosphates; D007527:Isoenzymes; D006593:Hexokinase