Class, amounts and affinities of anti-dinitrophenyl antibodies in chickens. I. Production of 7S and 17S antibodies of equal affinity by intravenous injection of antigen.
Repeated intravenous injections of maximally coupled dinitrophenylated bovine gamma-globulin elicited both 7S and 17S anti-dinitrophenyl antibodies in chickens. Only antibodies of low affinity were produced regardless of the priming dose, the interval between injections, and the number of injections. The 7S and 17S antibodies isolated from invididual animals had identical affinities and heterogeneity indices. The concentrations of antibodies formed were uniformly low despite many injections over a prolonged period. These studies indicate that stimulation by antigen alone may not be sufficient for the induction of predominant 7S antibody formation and for the synthesis of high affinity antibody.
D000327:Adsorption; D000818:Animals; D000917:Antibody Formation; D000937:Antigen-Antibody Reactions; D002417:Cattle / Q000276:immunology; D002645:Chickens; D003956:Dialysis; D004140:Dinitrophenols / Q000276:immunology*; D006801:Humans; D007115:Immunization Schedule; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G; D007457:Iodine Radioisotopes; D008239:Lysine / Q000378:metabolism; D012685:Sepharose; D012709:Serum Albumin; D014316:Tritium; D014461:Ultracentrifugation; D005719:gamma-Globulins
D004140:Dinitrophenols; D007074:Immunoglobulin G; D007457:Iodine Radioisotopes; D012709:Serum Albumin; D005719:gamma-Globulins; D014316:Tritium; D012685:Sepharose; D008239:Lysine