Alteration in the surface morphology of synchronized B-16 melanoma cell during the cell cycle.
B-16 melanoma cells were synchronized for G1-S periods by mitotic shake-off and S-G2 periods by thymidine block. The surface morphology of the B-16 melanoma at low density partially confluent culures revealed that, as in other cell systems investigated, the surface morphology could be correlated with four periods to cell cycle: G1, S, G2 and M. G1 cells were rounded and possessed microvilli, blebs and ruffles (late G1). During S period, blebs and microvilli diminished and disappeared, but ruffles were more evident. Late G2 cells acquired microvilli, surface blebs, and extended filopodia and began to thicken and round themselves for mitosis.
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000648:ultrastructure*; D002471:Cell Transformation, Neoplastic; D004273:DNA, Neoplasm / Q000096:biosynthesis; D008545:Melanoma / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008938:Mitosis; D009374:Neoplasms, Experimental
D004273:DNA, Neoplasm