title: On the nature of the external surface of cultured human embryo fibroblasts: an ultrastructural and cytochemical analysis utilising stain and lectin probes.
abstract: Surfaces of human embryo fibroblasts in vitro were reacted with stain and lectin probes for carbohydrate moeities either after or in the absence of treatment with low concentrations of surface-active enzymes and EDTA. Only testicular hyaluronidase significantly suppressed affinity-binding of all three agents, suggesting that acidic glycosaminoglycans are principle components of the cell's exterior.
mesh_terms: D000818:Animals; D001665:Binding Sites; D002241:Carbohydrates / Q000302:isolation & purification; D002417:Cattle; D002462:Cell Membrane / Q000032:analysis* / Q000201:enzymology / Q000648:ultrastructure; D003208:Concanavalin A / Q000302:isolation & purification; D004622:Embryo, Mammalian / Q000166:cytology; D004798:Enzymes / Q000378:metabolism; D005347:Fibroblasts / Q000032:analysis* / Q000648:ultrastructure; D006025:Glycosaminoglycans / Q000302:isolation & purification; D006801:Humans; D037102:Lectins / Q000032:analysis; D013194:Staining and Labeling; D013499:Surface Properties; D013501:Surface-Active Agents
chemical_list: D002241:Carbohydrates; D004798:Enzymes; D006025:Glycosaminoglycans; D037102:Lectins; D013501:Surface-Active Agents; D003208:Concanavalin A