[Effect of heat shock on the amino acid makeup of the whole cells and walls of Candida utilis yeasts in chemostat cultivation].
The amino acid composition of intact cells and cell walls was determined in Candida utilis AUCMY-1,668 growing in the regime of chemostat with limitation by glycerol or ethanol deficiency at a temperature of 30 degrees C (control) or with inhibition by an elevated temperature of 40 degrees C (experiment). In the control, intact cells contained 43-44% of amino acids, and cell walls, about 10% (per the weight of dry cell walls); the following amino acids prevailed in the cell walls: threonine, glutamic acid, serine and leucine. The content of amino acids decreased in both the intact cells and cell walls at the elevated temperature (40 degrees C). The content of leucine, methionine, tyrosine and cystine decreased in the cell walls more than in the intact cells (with regard to the total amino acid content of the cell walls and intact cells, respectively). Under the action of the elevated temperature, the cells became larger and did not separate: the scar formed at the end of budding stretched between the mother and daughter cells holding them together.
D000596:Amino Acids / Q000032:analysis*; D002175:Candida / Q000032:analysis*; D002473:Cell Wall / Q000032:analysis; D003470:Culture Media; D000431:Ethanol / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D013696:Temperature
D000596:Amino Acids; D003470:Culture Media; D000431:Ethanol; D005990:Glycerol
Vliianie teplovogo shoka na aminokislotnyĭ sostav tselykh kletok i stenok drozhzheĭ Candida utilis pri khemostatnom kul'tivirovanii.