Lymphoblastoid transformation and kinetics of appearance of viral nuclear antigen (EBNA) in cord-blood lymphocytes infected by Epstein-Barr Virus (EBV).
Human cord-blood lymphocytes were infected with B95.8 Epstein-Barr virus (EBV) before and after separation into B- and T-cell populations. Lymphoblastoid cells exhibiting B-cell characteristics appeared after 2 to 3 days of culture in the total population and in the separated B-cell subpopulation but not in the T-cell subpopulation. EBV nuclear antigen (EBNA) was detected concurrently with the appearance of lymphoblastoid cells. The proportion of EBNA-positive cells corresponded to that of lymphoblastoid cells, and reached 50% after 4 days. EBNA was present only in cells with B-cell markers. These observations indicate that only B-cells are susceptible to EBV infection, that the transformation occurs within a few days and that EBNA is a valid early marker for susceptibility to EBV transformation.
D000956:Antigens, Viral; D001402:B-Lymphocytes / Q000276:immunology; D001769:Blood; D002462:Cell Membrane / Q000276:immunology; D002467:Cell Nucleus / Q000276:immunology*; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D003168:Complement Fixation Tests; D004247:DNA / Q000096:biosynthesis; D004261:DNA Replication; D000939:Epitopes; D004854:Herpesvirus 4, Human / Q000276:immunology*; D006801:Humans; D007104:Immune Adherence Reaction; D007136:Immunoglobulins; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D014470:Umbilical Cord; D014779:Virus Replication
D000956:Antigens, Viral; D000939:Epitopes; D007136:Immunoglobulins; D004247:DNA