Hybridization of pigeon globin messenger RNA with complementary DNA synthesized in vitro by reverse transcription: influence of the homopolymeric regions.
The kinetics of hybridization of pigeon globin messenger RNA with complementary cDNA synthesized by means of AMV reverse transcriptase is complex. Addition of poly A or poly U in excess to the reaction mixture normalized the kinetics. It is concluded that association of the complementary homopolymeric regions of mRNA and cDNA accelerates the complex formation between heteropolymeric sequences in a fraction of the molecules.
D000227:Adenine Nucleotides; D000818:Animals; D009189:Avian Myeloblastosis Virus / Q000201:enzymology; D010856:Columbidae; D004247:DNA / Q000096:biosynthesis*; D003851:Deoxyribonucleases; D005914:Globins; D007700:Kinetics; D009693:Nucleic Acid Hybridization; D011072:Poly U; D011119:Polynucleotides; D012333:RNA, Messenger / Q000097:blood; D012194:RNA-Directed DNA Polymerase; D012156:Reticulocytes
D000227:Adenine Nucleotides; D011119:Polynucleotides; D012333:RNA, Messenger; D011072:Poly U; D005914:Globins; D004247:DNA; D012194:RNA-Directed DNA Polymerase; D003851:Deoxyribonucleases