Generation of electric current by chromatophores of Rhodospirillum rubrum and reconstitution of electrogenic function in subchromatophore pigment-protein complexes.
Lipoprotein complexes, containing (1) bacteriochlorophyll reaction centers, (2) bacteriochlorophyll light-harvesting antenna or (3) both reaction centers and antenna, have been isolated from chromatophores of non-sulphur purple bacteria Rhodospirillum rubrum by detergent treatments. The method of reconstituting the proteoliposomes containing these complexes is described. Being associtated with planas azolectin membrane, ptoteoliposomes as well as intact chromatophores were found to generate a light-dependent transmembrane electric potential difference measured by Ag/AgC1 electrodes and voltmeter. The direction of the electric field inproteoliposomes can be regulated by the addition of antenna complexes to the reconstitution mixture. The reaction center complex proteoliposomes generate an electric field of a direction opposite to that in chromatophores, whereas proteoliposomes containing reaction center complexes and a sufficient amount of antenna complexes produce a potential difference as in chromatophores. ATP and inorganic pyrophosphate, besides light, were shown to be usable as energy sources for electric generation in chromatophores associated with planar membrane.
D020130:Bacterial Chromatophores / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D001426:Bacterial Proteins / Q000378:metabolism*; D001665:Binding Sites; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D004553:Electric Conductivity; D006096:Gramicidin / Q000494:pharmacology; D008027:Light; D008074:Lipoproteins / Q000378:metabolism; D008081:Liposomes; D008854:Microscopy, Electron; D010860:Pigments, Biological / Q000378:metabolism*; D011485:Protein Binding; D012247:Rhodospirillum rubrum / Q000187:drug effects / Q000378:metabolism*; D013053:Spectrophotometry; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
D001426:Bacterial Proteins; D008074:Lipoproteins; D008081:Liposomes; D010860:Pigments, Biological; D006096:Gramicidin; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone