Effect of stabilizers on diaminobenzidine reactions of mitochondria.
The effect of various stabilizers--0.9% NaCl, 7.5% sucrose and 7.5% polyvinyl pyrrolidone (PVP) on reactions of mitochondria with fresh and photooxidized diaminobenzidine (DAB) was investigated. NaCl and PVP abolished the DAB staining of mitochondria incubated without exogenous cytochrome c; NaCl, however, was ineffective when exogenous cytochrome c or oxidized DAB were present in the incubation medium. PVP considerably decreased the intensity of all reactions performed in the presence of exogenous cytochrome c or oxidized DAB. The mechanism of the observed effect is not likely to involve osmotic protection of mitochondria; extraction of endogenous cytochrome c from fresh frozen tissue by NaCl and greatly increased viscosity of PVP-containing media seem to be most probable explanations.
D015100:3,3'-Diaminobenzidine; D000611:Aminobiphenyl Compounds; D000818:Animals; D003574:Cytochrome c Group / Q000032:analysis; D004355:Drug Stability; D066298:In Vitro Techniques; D051379:Mice; D008928:Mitochondria / Q000187:drug effects* / Q000201:enzymology; D009996:Osmotic Fragility; D010084:Oxidation-Reduction; D011205:Povidone / Q000494:pharmacology; D011817:Rabbits; D051381:Rats; D012965:Sodium Chloride / Q000494:pharmacology; D013194:Staining and Labeling; D013395:Sucrose / Q000494:pharmacology
D000611:Aminobiphenyl Compounds; D003574:Cytochrome c Group; D015100:3,3'-Diaminobenzidine; D012965:Sodium Chloride; D013395:Sucrose; D011205:Povidone