[Detection of specific antigen in the white blood cells of cattle with leukemia by a direct fluorescent antibody method (author's transl)].
The direct fluorescent antibody technique was used for detection of "specific" antigen in leukocytes of cattle with different forms of leukomia. Using fluorescein isothicoyanate-labeled immunoglobulins from colosterum of cows hyperimmunized with leukocyte mass from cattle with acute leukemia (hemocytobastosis), the "specific" antigen was detected in the cytoplasm of lymphoid and myeloid cells of animals with this and other forms of leukemia. This antigen was not detected in nuclear cells of the peripheral blood wither in normal animals or in animals with non-leukemic diseases (brucellosis, tuberculosis, various helminthic diseases) accompained by qualitative and quantitative changes in the blood similar to those leukemia.
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D000951:Antigens, Neoplasm / Q000032:analysis*; D002007:Brucellosis, Bovine / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology*; D003126:Colostrum / Q000276:immunology; D000939:Epitopes; D005260:Female; D005455:Fluorescent Antibody Technique / Q000379:methods*; D006373:Helminthiasis / Q000276:immunology; D007114:Immunization; D007136:Immunoglobulins / Q000032:analysis; D007938:Leukemia / Q000276:immunology*; D007945:Leukemia, Lymphoid / Q000276:immunology; D015470:Leukemia, Myeloid, Acute / Q000276:immunology; D007962:Leukocytes / Q000276:immunology*; D014380:Tuberculosis, Bovine / Q000276:immunology
D000906:Antibodies; D000951:Antigens, Neoplasm; D000939:Epitopes; D007136:Immunoglobulins
Obnaruzhenie spetsificheskogo antigena v kletkakh beloĭ krovi bol'nogo leĭkozom krupnogo rogatogo skota priamym metodom fliuorestsiruiushchikh antitel