title: Use of polyethylene glycol-treated calf serum for cell cultures in virus and interferon studies.
abstract: Immunoglobulins and lipoproteins can be efficiently removed from calf serum by precipitation with polyethylene glycol (PEG) 6000 under sterile conditions. The PEG-treated serum is suitable for cell cultures used for virus growth and assays. Moreover, PEG was found to slow down the growth of L cells and to enhance the production and activity of mouse interferon.
mesh_terms: D000818:Animals; D001769:Blood; D002417:Cattle; D002460:Cell Line; D002478:Cells, Cultured; D011232:Chemical Precipitation; D002642:Chick Embryo; D003470:Culture Media; D004622:Embryo, Mammalian; D004686:Encephalomyocarditis virus / Q000254:growth & development; D007136:Immunoglobulins / Q000302:isolation & purification; D007369:Interferon Inducers; D007739:L Cells; D008074:Lipoproteins / Q000302:isolation & purification; D051379:Mice; D011092:Polyethylene Glycols; D051381:Rats; D018139:Simplexvirus / Q000254:growth & development; D012845:Sindbis Virus / Q000254:growth & development; D014721:Vesicular stomatitis Indiana virus / Q000254:growth & development; D014776:Virus Cultivation; D005719:gamma-Globulins / Q000302:isolation & purification
chemical_list: D003470:Culture Media; D007136:Immunoglobulins; D007369:Interferon Inducers; D008074:Lipoproteins; D005719:gamma-Globulins; D011092:Polyethylene Glycols