Synthesis of highly diversified carrier ampholytes. Evaluation of the resolving power of isoelectric focusing in the Pi system (alpha-1-antitrypsin genetic polymorphism).
The use of condensing reagents such as epoxypropanol, diepoxyoctane, acrylamide and N,N'-methylenebisacrylamide in the synthesis of carrier ampholytes increased the diversity of amphoteric components. The quality of these synthetic carrier ampholytes has been tested in the separation of variants of alpha-1-anti-trypsin, a genetic polymorphism called the Pi system. A resolving power of the order of 0.005 pH unit was obtained.
D000665:Ampholyte Mixtures / Q000138:chemical synthesis*; D002021:Buffers / Q000138:chemical synthesis*; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D010641:Phenotype; D011110:Polymorphism, Genetic; D000515:alpha 1-Antitrypsin / Q000235:genetics / Q000302:isolation & purification*
D000665:Ampholyte Mixtures; D002021:Buffers; D000515:alpha 1-Antitrypsin