Pectinolytic enzymes of large rumen treponemes.
Large spiral organisms isolated from the rumen of cattle produced and released into the external environment a complex of pectinolytic enzymes, consisting mainly of poly(1,4-alpha-D-galacturonide) lyase (EC 4.2.2.2, formerly EC 4.2.99.3), most active at pH 8.0 to 9.0, and another enzyme acting at pH below 7.0, probably a poly(1,4-alpha-D-galacturonide) glycanohydrolase (EC 3.2.1.15). The mixture of enzymes degraded polygalacturonate to saturated and unsaturated monogalacturonates as the end products. A pectin pectylhydrolase (pectinesterase) (EC 3.1.1.11) was also present in the clarified cultures.
D000818:Animals; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D002417:Cattle; D004492:Edetic Acid / Q000494:pharmacology; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D010368:Pectins / Q000378:metabolism; D011096:Polygalacturonase / Q000378:metabolism*; D011133:Polysaccharide-Lyases / Q000378:metabolism*; D012417:Rumen / Q000382:microbiology*; D013379:Substrate Specificity; D014208:Treponema / Q000201:enzymology*; D014574:Uronic Acids / Q000378:metabolism
D014574:Uronic Acids; D010368:Pectins; D004492:Edetic Acid; D002265:Carboxylic Ester Hydrolases; D006026:Glycoside Hydrolases; D011096:Polygalacturonase; D011133:Polysaccharide-Lyases