Studies on the mobilization of iron from ferritin by isolated rat liver mitochondria.
Rat liver mitochondria and rat liver mitoplasts mobilize iron from ferritin by a mechanism which depends on a respiratory substrate (preferentially succinate), a small molecular weight electron mediator (FMN, phenazine methosulphate or methylene blue) and (near) anaerobic conditions. The release process under optimized conditions (approx. 50 mumol/1 FMN, 1 mmol/l succinate, 0.35 mmol/1 Fe(III) (as ferritin iron), 37 degrees C and pH 7.40) amounts to 0.9--1.2 nmol iron/mg protein per min. The results suggest that ferritin might function as an intermediate in the cytosolic transport of iron to the mitochondria.
D000818:Animals; D000968:Antimycin A / Q000494:pharmacology; D002264:Carboxylic Acids / Q000494:pharmacology; D004396:Coloring Agents; D003486:Cyanides / Q000494:pharmacology; D005293:Ferritins / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000378:metabolism*; D009243:NAD / Q000494:pharmacology; D010084:Oxidation-Reduction; D010100:Oxygen / Q000494:pharmacology; D010618:Phenanthrolines / Q000494:pharmacology; D051381:Rats; D012402:Rotenone / Q000494:pharmacology; D013347:Subcellular Fractions / Q000378:metabolism; D013696:Temperature
D002264:Carboxylic Acids; D004396:Coloring Agents; D003486:Cyanides; D010618:Phenanthrolines; D012402:Rotenone; D009243:NAD; D000968:Antimycin A; D005293:Ferritins; D007501:Iron; D010100:Oxygen