title: Intravascular effects of IgE antibody upon basophils,, neutrophils, platelets and blood coagulation in the rabbit.
abstract: Rabbits synthesizing only IgE antibody to BSA were challenged intravenously with 50 mg BSA and the associated blood cell and coagulation alterations were examined. Basophils decreased by 90% within 1 min of challenge. This was followed by a 70% decrease in neutrophils and a 50% decrease in platelets by 15 min. These changes were found to be highly significant when compared to control unimmunized rabbits similarly challenged. By 60 min, the neutrophils and platelets in the experimental rabbits had returned to 50 and 80% of their prechallenge levels respectively, but no basophils were demonstrable by toluidine blue staining. Lymphocyte counts in the experimental rabbits did not differ from controls at 0, 1, 15, or 60 min after challenge. Blood coagulation alterations also occurred after BSA challenge in the rabbits synthesizing IgE. A significant shortening of the whole blood clotting time in plastic tubes at 25 degrees C occurred in blood samples taken at 30 sec after antigen challenge. Similarly, in vitro addition of BSA to blood samples taken prior to challenge significantly shortened the whole blood clotting time. In addition, significant prolongation of whole blood clotting times was observed in blood obtained 60 min after challenge, but only among the rabbits positive for systemic anaphylaxis. This in vivo study corroborated the known in vitro effects of IgE upon the rabbit blood basophil and platelet. In addition, the results indicate that the intravascular interaction of antigen with specific IgE antibody induced neutrophil and blood coagulation alterations.
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D000917:Antibody Formation; D000937:Antigen-Antibody Reactions; D001491:Basophils / Q000276:immunology*; D001777:Blood Coagulation; D001780:Blood Coagulation Tests; D001792:Blood Platelets / Q000276:immunology*; D005260:Female; D006636:Histamine Release; D007114:Immunization; D007117:Immunization, Secondary; D007073:Immunoglobulin E / Q000502:physiology*; D007958:Leukocyte Count; D008297:Male; D009504:Neutrophils / Q000276:immunology*; D010323:Passive Cutaneous Anaphylaxis; D011817:Rabbits; D012710:Serum Albumin, Bovine
chemical_list: D012710:Serum Albumin, Bovine; D007073:Immunoglobulin E