The mechanism of NADPH-dependent lipid peroxidation. The propagation of lipid peroxidation.
NADPH-dependent lipid peroxidation occurs in two distinct sequential radical steps. The first step, initiation, is the ADP-perferryl ion-catalyzed formation of low levels of lipid hydroperoxides. The second step, propagation, is the iron-catalyzed breakdown of lipid hydroperoxides formed during initiation generating reactive intermediates and products characteristic of lipid peroxidation. Propagation results in the rapid formation of thiobarbituric acid-reactive material and lipid hydroperoxides. Propagation can be catalyzed by ethylenediamine tetraacetate-chelated ferrous ion, diethylenetriamine pentaacetic acid-chelated ferrous ion, or by ferric cytochrome P-450. However, cytochrome P-450 is destroyed during propagation.
D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004492:Edetic Acid / Q000494:pharmacology; D007501:Iron / Q000494:pharmacology; D050356:Lipid Metabolism; D008081:Liposomes; D008084:Lipoxygenase; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D009249:NADP; D010544:Peroxidases / Q000378:metabolism; D010545:Peroxides / Q000378:metabolism*; D051381:Rats; D013482:Superoxide Dismutase
D008081:Liposomes; D010545:Peroxides; D009249:NADP; D003577:Cytochrome P-450 Enzyme System; D004492:Edetic Acid; D007501:Iron; D010544:Peroxidases; D008084:Lipoxygenase; D013482:Superoxide Dismutase