Generation of free radicals from phenazine methosulfate in Trypanosoma cruzi epimastigotes.
A significant growth inhibition of Trypanosoma cruzi epimastigotes by phenazine methosulfate (PMS) was observed in Warren's medium. This toxic activity could be related to the following parameters: a) formation of phenazinium free radical, b) generation of superoxide anion in intact cells incubated with PMS, and c) PMS also increased significantly the rate of O2- generation in epimastigotes mitochondrial and microsomal fractions using NADH as electron donor.
D004578:Electron Spin Resonance Spectroscopy; D008773:Methylphenazonium Methosulfate / Q000494:pharmacology*; D008861:Microsomes / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism*; D010619:Phenazines / Q000494:pharmacology*; D013057:Spectrum Analysis; D013481:Superoxides / Q000378:metabolism*; D014349:Trypanosoma cruzi / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure
D010619:Phenazines; D013481:Superoxides; D008773:Methylphenazonium Methosulfate; D010100:Oxygen