Rapid test for screening of narcotic analgesics in mice.
50 microliter of a solution of 0.35 N NH4OH containing 2.325% EDTA and 0.837% NaCl injected intradermally in mice elicited reproducible short term behavior typical for nociceptive stimulus. This behavior was inhibited specifically by narcotic analgesics. This procedure may be used as a specific test for screening and for dose--effect studies of narcotic analgetic drugs in mice.
D000701:Analgesics, Opioid / Q000494:pharmacology*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D004492:Edetic Acid / Q000037:antagonists & inhibitors; D005260:Female; D051379:Mice
D000701:Analgesics, Opioid; D004492:Edetic Acid