A simple two-step isolation procedure for human and bovine antithrombin II/III (heparin cofactor): a comparison of two methods.
Human and bovine antithrombin II/III have been isolated by a simple procedure essentially using only affinity chromatography on heparin-agarose and polyethylene glycol precipitation. An additional ammonium sulphate step removes polyethylene glycol and trace contaminants. The final preparation is homogeneous by SDS-, disc- and agar slide electrophoresis. End group analysis of human antithrombin II/III shows histidine as the N-terminal amino acid. The pure preparation showed both progressive antithrombin activity and heparin cofactor activity.
D000510:Alpha-Globulins / Q000302:isolation & purification*; D000818:Animals; D000990:Antithrombin III / Q000032:analysis / Q000302:isolation & purification*; D002417:Cattle; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004355:Drug Stability; D005026:Ethylene Glycols; D005782:Gels; D006493:Heparin / Q000032:analysis / Q000302:isolation & purification; D006639:Histidine / Q000032:analysis; D006801:Humans; D012685:Sepharose
D000510:Alpha-Globulins; D005026:Ethylene Glycols; D005782:Gels; D006639:Histidine; D000990:Antithrombin III; D006493:Heparin; D012685:Sepharose