[Comparative evaluation of methods for isolating total protein from the biomass of Spirulina platensis].
The methods of extracting total protein from the biomass of Spirulina platensis are discussed. The results of studying different procedures of cell wall disruption, soluble protein extraction and precipitation are presented. The best results can be obtained using mechanical disintegration of Spirulina cells for 20 min (at a temperature not higher than 25 degrees C), soluble protein extraction with 0.4% NaOH, and its subsequent precipitation at the isoelectric point with 5% HCl.
D001419:Bacteria / Q000032:analysis*; D001426:Bacterial Proteins / Q000302:isolation & purification*; D005069:Evaluation Studies as Topic; D006863:Hydrogen-Ion Concentration; D008722:Methods; D013696:Temperature
D001426:Bacterial Proteins
Sravnitel'naia otsenka metodov vydeleniia summarnogo belka iz biomassy Spirulina Plastensis.