title: Absence of binding of pancreatic and urinary kallikreins to alpha 2-macroglobulin.
abstract: Pancreatic and urinary kallikreins failed to form the typical serine proteinase complex with alpha2M (alpha2-macroglobulin). Studies were performed to compare this with the binding of trypsin to alpha2M at various molar binding ratios, with the use of Sephadex G-200 gel filtration to separate free and alpha2M-bound enzyme fractions. The subunit conversion was totally absent with pancreatic kallikrein from lhich traces of a binding proteinase had been removed. The lack of binding is believed to be the result of the restricted specificity of the kallikreins.
mesh_terms: D055598:Chemical Phenomena; D002621:Chemistry; D002850:Chromatography, Gel; D006801:Humans; D007610:Kallikreins / Q000652:urine; D010179:Pancreas; D011485:Protein Binding; D000511:alpha-Macroglobulins
chemical_list: D000511:alpha-Macroglobulins; D007610:Kallikreins