In vitro response of mouse spleen cells to the solid phase immunogen DNP-O-Bio-Gel.
The in virto immunogenicity of the solid-phase hapten, dinitrophenyl-ornithine-Bio-Gel (DNP-O-Bio-Gel), was investigated in cultures of mouse spleen cells. Appropriate combinations of cells and immobilized hapten were determined. Large numbers of direct anti-hapten plaque-forming cells (PFC) were generated when 1 times 10-7 C57BL/6 or C57BL/10 spleen cells were cultured with 4 times 10-3 DNP-O-Bio-Gel beads. Specificity studies of the responses of cultured spleen cells to DNP-O-Bio-Gel yielded the following results: soluble DNP-ornithine or DNP-bovine gamma-globulin inhibited the induction of anti-hapten PFC by DNP-O-Bio-Gel; neither dinitrophenyl-Bio-gel (DNP-Bio-gel) nor ornithine-Bio-Gel (O-Bio-Gel) induced anti-hapten responsiveness; furthermore, neither DNP-Bio-Gel nor O-Bio-Gel inhibited the induction of PFC by DNP-O-Bio-Gel. It was concluded, from the results of these specificity experiments, that a spacer, ornithine, is required for immunogenicity of immobilized DNP; and that the Bio-Gel bead, itself, acts solely as a physical carrier for the hapten.
D000178:Acrylamides / Q000276:immunology*; D000818:Animals; D000939:Epitopes; D004912:Erythrocytes / Q000276:immunology; D005838:Genotype; D006241:Haptens; D006462:Hemolytic Plaque Technique; D007111:Immunity, Cellular; D008214:Lymphocytes / Q000276:immunology*; D009578:Nitrobenzenes / Q000276:immunology*; D009952:Ornithine / Q000276:immunology*; D012756:Sheep / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology*
D000178:Acrylamides; D000939:Epitopes; D006241:Haptens; D009578:Nitrobenzenes; D009952:Ornithine