[Effect of gramicidin C on the formation and germination of Bacillus brevis var. GB (P+-variant) spores].
The effect of gramicidin C added to the medium at various periods of cultivation in concentrations of 20, 40 and 100 gamma/ml on sporulation of P+-variant of Bac. brevis var. GB was studied. The most effective increase in the sporulation rate and percentage of the cells germinating into the spores was observed on addition of the antibiotic to the medium in amounts of 20 and 40 gamma/ml in 13 hours of the culture development. The amount of gramicidin C during sporulation decreased and partially passed into the spores which did not differ after germination from those of P+-variant grown on the synthetic medium with glucose and without preliminary addition of the antibiotic. Addition of gramicidin C in an amount of 100 gamma/ml at the end of the lag phase, i.e. 4 hours after the culture inoculation suppressed sporulation and had no effect on growth of the cells of its own producing organism.
D000409:Alanine / Q000378:metabolism; D001407:Bacillus / Q000187:drug effects* / Q000254:growth & development; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D014644:Genetic Variation / Q000187:drug effects*; D006096:Gramicidin / Q000494:pharmacology*; D013171:Spores, Bacterial / Q000187:drug effects* / Q000254:growth & development; D013997:Time Factors
D003470:Culture Media; D006096:Gramicidin; D000409:Alanine
Vliianie gramitsidina C na obrazovanie i prorastanie spor Bacillus brevis var. GB (P+-variant)