Chromatography of xanthines on ion-exchange resins.
Short columns of a 4% crosslinked cation-exchange resin gave good chromatography of xanthines, including caffeine, theophylline and hypoxanthine, and related polar aromatic compounds. Elution volumes and sequences can be modified by changing pH, solvent composition and resin counter-ion. A macroporous cation-exchange resin showed exaggerated counter-ion effects. A method is described for determining caffeine and theophylline in blood serum, using the 4% crosslinked resin with aqueous sodium phosphate eluent of pH 7.5; the temperature was 65 degrees. Detection limits are 10 ng and less.
D002110:Caffeine / Q000032:analysis / Q000097:blood; D002411:Cation Exchange Resins; D002852:Chromatography, Ion Exchange; D003069:Coffee; D006801:Humans; D006863:Hydrogen-Ion Concentration; D013056:Spectrophotometry, Ultraviolet; D013806:Theophylline / Q000097:blood; D014970:Xanthines / Q000097:blood*
D002411:Cation Exchange Resins; D003069:Coffee; D014970:Xanthines; D002110:Caffeine; D013806:Theophylline