Preparation of monospecific antiserums against porcine immunoglobulins, using agarose-linked immunosorbents.
Immunoglobulins IgG, IgA, and IgM were isolated from porcine serum and milk, and antiserums against the 3 immunoglobulin classes were prepared. Monospecificity of the antiserums for the gamma-, alpha-, and mu-chains was obtained by absorbing them in agarose-linked immunosorbent columns. These immunosorbents were prepared by linking IgG or IgA-IgM to CNBr-activated agarose. Contaminating anti-alpha2-macroglobulin antibodies in the anti-IgA and anti-IgM serums were removed with agarose-linked fetal globulins.
D000042:Absorption; D000818:Animals; D003429:Cross Reactions; D007106:Immune Sera; D007122:Immunoelectrophoresis; D007070:Immunoglobulin A / Q000302:isolation & purification; D007074:Immunoglobulin G / Q000302:isolation & purification; D007075:Immunoglobulin M / Q000302:isolation & purification; D007137:Immunoglobulin alpha-Chains / Q000302:isolation & purification; D007148:Immunoglobulin mu-Chains / Q000302:isolation & purification; D007136:Immunoglobulins / Q000302:isolation & purification*; D008722:Methods; D008892:Milk / Q000276:immunology; D012685:Sepharose; D013552:Swine / Q000276:immunology*; D000511:alpha-Macroglobulins / Q000302:isolation & purification
D007106:Immune Sera; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007137:Immunoglobulin alpha-Chains; D007148:Immunoglobulin mu-Chains; D007136:Immunoglobulins; D000511:alpha-Macroglobulins; D012685:Sepharose