NAD-linked glutamate dehydrogenase in Trypanosoma cruzi.
1. Epimastigotes of Trypanosoma cruzi, Tulahuén strain, contained a NAD-linked glutamate dehydrogenase (EC 1.4.1.3), in addition to the already known NADP-linked enzyme enzyme (EC 1.4.1.4). 2. The partially purified NAD-linked enzyme had a higher molecular weight and was much more labile than the NADP-linked enzyme, and was inhibited by purine nucleotides. 3. These results further emphasize the difference in glutamate metabolism between the parasite and its mammalian host.
D000818:Animals; D002474:Cell-Free System; D002850:Chromatography, Gel; D005969:Glutamate Dehydrogenase / Q000302:isolation & purification / Q000378:metabolism*; D009243:NAD / Q000378:metabolism*; D009249:NADP / Q000378:metabolism; D014349:Trypanosoma cruzi / Q000201:enzymology*
D009243:NAD; D009249:NADP; D005969:Glutamate Dehydrogenase