Effects of (Na+ + K+)-ATPase-specific antibodies of enzymatic activity and monovalent cation transport.
Antibodies have been obtained that specifically interact with the transport enzyme (Na+ + K+)-activated ATPase. The antigen used was purified (Na+ + Ka+)-ATPase from canine renal medulla. Purified gamma globulin from immunized animals, but not from control animals or preimmune serum, inhibited (Na+ + Ka+)-ATPase from canine renal medullar with reduction of activity to 33 +/- 4 (SD)% in concentration-dependent manner. Maximum inhibition occurred in less than 5 minutes at 37 degrees C. The Mg++ -dependent, nonouabain inhibited component of activity (Mg++ -ATPase) was unaffected. Fab fragments obtained by papain cleavage of the gamma globulin fraction had similar inhibitory activity and specificity. These antibodies also produced varying degrees of concentration-related inhibition of canine myocardial, calf brain, and human red cell ghost (Na++ + Ka+)-ATPase, but not Mg++-ATPase activity.
D000251:Adenosine Triphosphatases / Q000276:immunology* / Q000378:metabolism; D000818:Animals; D000906:Antibodies; D001693:Biological Transport, Active; D001921:Brain / Q000201:enzymology; D002417:Cattle; D002462:Cell Membrane / Q000201:enzymology; D004285:Dogs; D000939:Epitopes; D004912:Erythrocytes / Q000201:enzymology; D006168:Guinea Pigs; D006461:Hemolysis; D006801:Humans; D007679:Kidney Medulla / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology; D012413:Rubidium / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*; D005719:gamma-Globulins / Q000494:pharmacology
D000906:Antibodies; D000939:Epitopes; D005719:gamma-Globulins; D012964:Sodium; D000251:Adenosine Triphosphatases; D012413:Rubidium