Extrusion of sodium ions energized by respiration and glycolysis in Escherichia coli.
Extrusion of sodium ion from cells of Escherichia coli was measured using an Na+ electrode. When oxygen was supplied to an anaerobic cell suspension, extrusion of Na+ was observed. The addition of glucose under anaerobic conditions also caused Na+ efflux. The extrusion of Na+ energized by respiration and glycolysis was completely inhibited by a proton conductor, carbonyl cyanide m-chlorophenylhydrazone. These observations are consistent with the view that Na+ transport occurs secondarily to H+ circulation. Interestgly, induction of the melibiose transport system, which is coupled to Na+, greatly enhanced Na+ transport activity.
D000693:Anaerobiosis; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*; D006019:Glycolysis / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010101:Oxygen Consumption / Q000187:drug effects; D012964:Sodium / Q000378:metabolism*
D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D012964:Sodium