Alpha-fetoprotein and albumin produced by subclonal cell population of the ascites hepatoma AH-66 in vitro.
Subclonal cell populations were prepared from a clonal line of the rat ascites hepatoma AH-66 in vitro, using the agar plate culture method. alpha-Fetoprotein and albumin concentrations in culture media of these subclones were determined by 125I-radioimmunoassay and single radial immunodiffusion method, respectively. Results demonstrated that the AH-66 clone was a complex of cells with varying producibility of alpha-fetoprotein and albumin. All the subclones showed varied and distinct production of alpha-fetoprotein, but not all the subclones produced detectable levels of albumin.
D000418:Albumins / Q000096:biosynthesis*; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D002999:Clone Cells; D005326:Fetal Proteins / Q000096:biosynthesis*; D008113:Liver Neoplasms; D009374:Neoplasms, Experimental; D000509:alpha-Fetoproteins / Q000096:biosynthesis*
D000418:Albumins; D005326:Fetal Proteins; D000509:alpha-Fetoproteins