Localization of tropomyosin in mouse embryo fibroblasts.
Antiserum to chick skeletal muscle tropomyosin was used to localize tropomyosin in mouse embryo fibroblasts by the indirect fluorescein labeled antibody technique. Specific staining was observed cytoplasmic fibers, which extended out into the cell processes. The staining pattern in these cells is similar to that previously described by others for actin. This observation suggests that in fibroblasts tropomyosin, like actin, is localized in fibers in the cytoplasm.
D000199:Actins / Q000378:metabolism; D000818:Animals; D002645:Chickens; D003593:Cytoplasm / Q000648:ultrastructure; D004591:Electrophoresis, Polyacrylamide Gel; D004622:Embryo, Mammalian; D004625:Embryo, Nonmammalian; D005347:Fibroblasts / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D051379:Mice; D009132:Muscles / Q000502:physiology; D011817:Rabbits; D013194:Staining and Labeling; D014335:Tropomyosin / Q000032:analysis*
D000199:Actins; D007106:Immune Sera; D014335:Tropomyosin