Type C RNA tumor virus isolated from cultured human acute myelogenous leukemia cells.
Previously, type C RNA tumor virus-related components have been described in blood leukocytes from patients with acute myelogenous leukemia. These components, for example, reverse transcriptase, have been shown to be most closely related to those from two oncogenic subhuman primate type C viruses (woolly monkey sarcoma virus and gibbon ape leukemia virus). Now, we report the continuous production of budding type C viruses with the same characteristic reverse transcriptase by three separate culturings of leukocytes from a single bleeding from a patient with acute myelogenous leukemia. These isolations were made possible by the discovery of a source of conditioned media which sustains exponential growth of human myelogenous leukemia cells in liquid suspension culture.
D002454:Cell Differentiation; D002455:Cell Division; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D003470:Culture Media; D000939:Epitopes; D005260:Female; D006801:Humans; D007118:Immunoassay; D007074:Immunoglobulin G; D015470:Leukemia, Myeloid, Acute / Q000382:microbiology*; D007962:Leukocytes / Q000382:microbiology; D008854:Microscopy, Electron; D008875:Middle Aged; D012194:RNA-Directed DNA Polymerase / Q000032:analysis; D012190:Retroviridae / Q000201:enzymology / Q000254:growth & development / Q000302:isolation & purification*
D003470:Culture Media; D000939:Epitopes; D007074:Immunoglobulin G; D012194:RNA-Directed DNA Polymerase