title: Production of antiserum to human properdin and demonstration of antigenic differences between the native and activated protein.
abstract: Antisera were raised in rabbits to human properdin in the precursor form (P) and in the activated state (P). On Ouchterlony analysis using the anti-P, reactions of complete identity were obtained between P, P, and properdin in twofold concentrated serum (NHS). However, when anti-P was used, a reaction of identity was obtained only between P and NHS, and a reaction of partial identity was formed between P and P and between properdin in NHS and P, suggestive of the fact that certain antigenic determinants in P may be lacking in P. The results indicate that activation of precursor properdin may involve proteolytic cleavage and/or conformational alterations of the molecule.
mesh_terms: D000818:Animals; D000906:Antibodies; D000939:Epitopes; D006801:Humans; D011414:Properdin / Q000276:immunology* / Q000502:physiology; D011487:Protein Conformation; D011817:Rabbits / Q000276:immunology; D013329:Structure-Activity Relationship
chemical_list: D000906:Antibodies; D000939:Epitopes; D011414:Properdin