Increased specific binding of [3H]diazepam in rat brain following chronic diazepam administration.
Male rats (175 g) were given 30 mg of diazepam in their food daily for 35 days. The animals became drowsy and ataxic from this high dose of drug. After the 35-day dosing, the rats were killed daily, and specific binding of [3H]diazepam and [3H]flunitrazepam was determined in synaptosomal preparations from these and corresponding control rats. On days 3, 4, 6, and 7 after the treatment period the specific binding and specific binding of [3H]diazepam was double that of the control binding and specific binding of [3H]flunitrazepam was 1.67 times that of control. The data indicate that very high doses of diazepam, given for long periods, cause increased specific binding of radiolabeled ligand to brain subfractions. The possible mechanisms and implications are discussed. When lower doses or shorter dosage regimens are used, increased binding is not observed.
D000818:Animals; D014151:Anti-Anxiety Agents / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D003975:Diazepam / Q000008:administration & dosage / Q000378:metabolism*; D004032:Diet; D005445:Flunitrazepam / Q000008:administration & dosage / Q000378:metabolism*; D008297:Male; D051381:Rats
D014151:Anti-Anxiety Agents; D005445:Flunitrazepam; D003975:Diazepam