Synthesis of alpha-fetoprotein by rat ascites hepatoma cells.
AH-66 rat ascites hepatoma cells incorporated [14C]leucine into the AFP fraction. In a cell-free system, hepatoma ribosomes were found to be active in AFP synthesis whereas the supernatant fraction from hepatoma had no specific effect on AFP production. The amount of [14C]leucine incorporated in AFP by membrane-bound polysomes was 20 to 90 times higher than that by free polysomes, suggesting that AFP is mainly synthesized on membrane-bound polysomes. DBcAMP inhibited the growth of hepatoma cells. However, the incorporation of [14C]leucine into the AFP fraction as well as into total proteins was stimulated by DBcAMP.
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D005326:Fetal Proteins / Q000096:biosynthesis*; D007700:Kinetics; D007930:Leucine / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008113:Liver Neoplasms; D008297:Male; D009374:Neoplasms, Experimental / Q000378:metabolism; D011132:Polyribosomes / Q000378:metabolism; D014176:Protein Biosynthesis / Q000187:drug effects; D051381:Rats; D012270:Ribosomes / Q000378:metabolism; D013806:Theophylline / Q000494:pharmacology; D000509:alpha-Fetoproteins / Q000096:biosynthesis*
D005326:Fetal Proteins; D000509:alpha-Fetoproteins; D003994:Bucladesine; D013806:Theophylline; D007930:Leucine