Lipase productivity of a lipolytic strain of Thermoactinomyces vulgaris.
An attempt was made to evaluate the lipase productivity by Thermoactinomyces vulgaris with various nutritional and environmental factors. Results suggest optimum conditions for production, viz. age of inoculum 18 hours, 55 degrees C, pH 6.8, 0.2% yeast extract, specific substrates, and shaken cultures incubated for 24--36 hours. Lipases are induced by corn oil in presence of yeast extract or of certain substitutes; starch could replace these at 55 degrees C. A suggestion is given of a regulatory system of lipase synthesis, involving the supply of starch to initiate growth, allowing induction of lipase by the inductive substrate.
D003470:Culture Media; D004534:Egypt; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000096:biosynthesis*; D008848:Micromonosporaceae / Q000201:enzymology*; D012988:Soil Microbiology; D013379:Substrate Specificity; D013696:Temperature
D003470:Culture Media; D008049:Lipase