title: Human antibodies to bovine alpha-globulin.
abstract: Antibodies to bovine gamma-globulin (anti-BGG antibodies) were detectable by a radio-immunoassay in 70% of healthy blood donors but, generally, the titres were low. Significantly increased concentrations of anti-BGG antibodies were found in patients lacking IgA but not in patients with allergic disorders. The anti-BGG antibodies were shown to give rise to falsely high IgE values in the radio-immunosorbent test for IgE determination (RIST) when a sheep anti-IgE antiserum was used. Furthermore, falsely positive results can sometimes be caused by such antibodies in the determination of cow-dander- or cow's-milk-specific IgE by the radio-allergosorbent test (RAST). When a rabbit anti-IgE antiserum was used instead of the sheep anti-IgE, normal IgE levels and negative RAST results were obtained. The difference is explained by the higher degree of cross-reactivity between the anti-BGG antibodies and sheep alpha-globulin than between anti-BGG antibodies and rabbit alpha-globulin.
mesh_terms: D000042:Absorption; D000485:Allergens; D000818:Animals; D000906:Antibodies / Q000032:analysis*; D002415:Cats; D002417:Cattle / Q000276:immunology; D002850:Chromatography, Gel; D003429:Cross Reactions; D004285:Dogs; D005189:False Positive Reactions; D006041:Goats / Q000276:immunology; D006168:Guinea Pigs; D006197:Hair; D006736:Horses / Q000276:immunology; D006801:Humans; D007106:Immune Sera; D007073:Immunoglobulin E / Q000032:analysis; D007141:Immunoglobulin Fc Fragments; D007457:Iodine Radioisotopes; D008892:Milk; D009194:Myeloma Proteins; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay; D012710:Serum Albumin, Bovine; D012756:Sheep / Q000276:immunology; D013552:Swine / Q000276:immunology; D005719:gamma-Globulins
chemical_list: D000485:Allergens; D000906:Antibodies; D007106:Immune Sera; D007141:Immunoglobulin Fc Fragments; D007457:Iodine Radioisotopes; D009194:Myeloma Proteins; D005719:gamma-Globulins; D012710:Serum Albumin, Bovine; D007073:Immunoglobulin E