DNA-synthesis on giant nuclear RNA by AMV DNA polymerase.
The reverse transcription of pre-mRNA isolated from rat liver or mouse Ehrlich ascites carcinoma cells with the aid of hot phenol fractionation technique is described. Pre-mRNA isolated at 85 degrees C is a more active template than the 65 degree C fraction. The addition of oligo(dT) as a primer strongly stimulated the template activity of the 65 degree C fraction. The size of product corresponds to a sedimentation value of 7 S as measured in alkaline sucrose gradient and is essentially less than the size of template.
D000818:Animals; D001354:Avian Leukosis Virus / Q000201:enzymology*; D009189:Avian Myeloblastosis Virus / Q000201:enzymology*; D002286:Carcinoma, Ehrlich Tumor; D002467:Cell Nucleus / Q000378:metabolism*; D002499:Centrifugation, Density Gradient; D004261:DNA Replication; D008099:Liver; D012333:RNA, Messenger / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*; D051381:Rats; D013696:Temperature
D012333:RNA, Messenger; D012194:RNA-Directed DNA Polymerase