Immunochemical studies of lipids. IV. Chemical modification of Forssman globoside and immunological activities.
N-deacylated and N-deacetylated Forssman globoside obtained from Forssman globoside by partial alkaline hydrolysis had no Forssman activity. After re-N-acetylation of the N-deacylated and N-deacetylated Forssman globoside, the N-acetylsphingosyl Forssman oligosaccharide thus obtained recoverd its activity. Moreover, after ozonolysis and reduction of the N-acetylsphingosyl Forssman oligosaccharide, the 2-N-acetoamido-1,3,4-trihydroxybutanoyl Forssman oligosaccharide thus obtained still had a haptenic activity, but couldn't show a precipitin reaction with Forssman antibody. On the other hand, the Forssman globoside and the N-acetylsphingosyl Forssman oligosaccharide gave rise to precipitin line on agar gel double diffusion, it was thus concluded that the aqueous solution of these substances formed spherical micelles to behave like a fairly macromolecular multivalent antigen.
D000107:Acetylation; D000215:Acylation; D002554:Cerebrosides; D055598:Chemical Phenomena; D002621:Chemistry; D002627:Chemistry, Physical; D000939:Epitopes; D005577:Forssman Antigen; D006241:Haptens; D006461:Hemolysis; D008956:Models, Chemical
D002554:Cerebrosides; D000939:Epitopes; D006241:Haptens; D005577:Forssman Antigen