B-cell mitogenic effects on human lymphocytes of rabbit anti-human beta 2-microglobulin.
Rabbit Ig against human beta2-microglobulin was found to be mitogenic for human peripheral lymphocytes, tonsil lymphocytes, and appendix and spleen cells. Anti-beta2-m gave increased DNA synthesis, with peak responses on day 3 or 4 and showed a dose-response effect when diluted. The effect was seen in both serum-free and serum-containing culture medium. Anti-beta2-m, as well as lipopolysaccharide, induced polyclonal antibody production and intracellular immunoglobulin synthesis in blast cells, which is taken as evidence that these substances are human B-cell mitogens. Anti-beta2-m, but not lipopolysaccharide, could, in these experiments, activate peripheral blood lymphocytes, in addition to lymphoid cells from other sources. Thus, anti-beta2-m can serve as a functional marker for peripheral human B lymphocytes.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D000917:Antibody Formation / Q000187:drug effects; D001065:Appendix / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D001609:Beta-Globulins / Q000494:pharmacology*; D002172:Canavanine / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D004926:Escherichia coli; D006801:Humans; D007106:Immune Sera; D037102:Lectins / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008938:Mitosis / Q000187:drug effects*; D014066:Palatine Tonsil / Q000276:immunology; D011135:Polysaccharides, Bacterial / Q000494:pharmacology; D011817:Rabbits; D012756:Sheep; D013154:Spleen / Q000276:immunology; D001613:beta 2-Microglobulin / Q000494:pharmacology*
D000888:Antibodies, Anti-Idiotypic; D001609:Beta-Globulins; D007106:Immune Sera; D037102:Lectins; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D001613:beta 2-Microglobulin; D002172:Canavanine; D004247:DNA