[Activation of plasminogen with immobilized trypsin].
Human plasminogen isolated from the placenta serum fraction by means of affinity chromatography was activated by trypsin being in covalent bond with sepharose. The activation is studied as dependent on pH, temperature and the proenzyme-activator ratio in the presence of 25% glycerol as a stabilizing agent and without it. Utilization of the immobilized trypsin as a plasminogen activator makes it possible to transform completely the proenzyme to plasmin varying the plasminogen-trypsin ratio and time of activation when it is conducted under optimal conditions: in the presence of 25% glycerol at pH 7.0-7.1 and the temperature of 30 degrees C.
D004789:Enzyme Activation; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005260:Female; D005312:Fetal Blood / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010920:Placenta; D010958:Plasminogen / Q000378:metabolism*; D011247:Pregnancy; D013696:Temperature; D014357:Trypsin / Q000378:metabolism*
D004800:Enzymes, Immobilized; D010958:Plasminogen; D014357:Trypsin
Aktivatsiia plazminogena immobilizovannym tripsinom.