Carrier-determined tolerance in vitro.
A primary immune response to normal BDF1 spleen cells was obtained in vitro to the T-independent antigen TNP-T4 coliphage. This anti-TNP response was suppressed by exposing the spleen cells for 6 hr to TNP bound either to isologous or heterologous gamma-globulin. The suppression was hapten specific. In contrast, TNP-albumin conjugates did not induce tolerance in vitro.
D000818:Animals; D002352:Carrier Proteins; D002417:Cattle / Q000276:immunology; D002478:Cells, Cultured; D003090:Coliphages / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology; D006241:Haptens; D006462:Hemolytic Plaque Technique; D006801:Humans; D007108:Immune Tolerance; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D009596:Nitrophenols / Q000276:immunology; D011817:Rabbits / Q000276:immunology; D012710:Serum Albumin, Bovine; D012756:Sheep / Q000276:immunology; D013154:Spleen / Q000166:cytology; D013950:Thymus Gland / Q000002:abnormalities; D005719:gamma-Globulins
D002352:Carrier Proteins; D006241:Haptens; D009596:Nitrophenols; D005719:gamma-Globulins; D012710:Serum Albumin, Bovine