title: Further characterization of the Friend murine leukemia virus reverse transcriptase-RNase H complex.
abstract: The purified reverse transcriptase-RNase H complex from Friend murine leukemia virus consists of a single polypeptide of 84,000 molecular weight, which after mild protease treatment in vitro or after intentional degradation during the purification procedure allows the generation of several additional polypeptides. Degradation destroys the RNA-dependent DNA polymerase activity with native RNA templates and reduces RNase H but does not affect response to synthetic template primers such as poly (rA)-Oligo (dT). The properties of the intact murine enzyme consisting of a single polypeptide of 84,000 molecular weight are compared to those of the avian alpha subunit and the avian alpha beta enzyme complex. The intact murine enzyme resembles the avian beta-containing enzyme complex and is different from alpha in the following respects: (i) it binds to native RNA templates; (ii) it transcribes native RNA templates into DNA, a reaction which can be inhibited by actinomycin D; (iii) RNase H activity behaves like a processive exonuclease; and (iv) analysis of the RNase H digestion products reveals oligonucleotides approximately four bases in length.
mesh_terms: D004279:DNA, Viral / Q000096:biosynthesis; D003609:Dactinomycin / Q000494:pharmacology; D005622:Friend murine leukemia virus / Q000201:enzymology*; D008970:Molecular Weight; D009097:Multienzyme Complexes / Q000032:analysis / Q000378:metabolism; D009841:Oligonucleotides / Q000378:metabolism; D010455:Peptides / Q000032:analysis; D012367:RNA, Viral / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase / Q000032:analysis / Q000378:metabolism; D012260:Ribonucleases / Q000032:analysis / Q000378:metabolism; D013698:Templates, Genetic; D014158:Transcription, Genetic
chemical_list: D004279:DNA, Viral; D009097:Multienzyme Complexes; D009841:Oligonucleotides; D010455:Peptides; D012367:RNA, Viral; D003609:Dactinomycin; D012194:RNA-Directed DNA Polymerase; D012260:Ribonucleases