Improvements and simplifications of the ozonolysis-Schiff staining method for plasma lipoproteins on cellulose acetate.
Modifications of the ozonolysis and Schiff staining of plasma lipoproteins reduce 11 pre-spectrometric steps to 3. Picrate denaturation of the lipoproteins and absorption spectrometry directly on the wet opaque membrane increase the sensitivity; the volume of sub-specimen needed per length of application site is reduced from 0.7 to 0.1 mul/mm. Reasonably good proportionality between the total lipoprotein concentration and the spectrometric findings are obtained. Two specimens were obtained at an interval of 8-13 days from each of 10 patients; for the arbitrary substance fraction of alpha-, prebeta-, and beta-lipoproteins in plasma the between-series intra-individual standard deviations were 0.021, 0.023 and 0.032 respectively.
D004588:Electrophoresis, Cellulose Acetate / Q000379:methods; D005069:Evaluation Studies as Topic; D006801:Humans; D007202:Indicators and Reagents; D008074:Lipoproteins / Q000097:blood*; D008075:Lipoproteins, HDL / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood; D008079:Lipoproteins, VLDL / Q000097:blood; D008433:Mathematics; D013194:Staining and Labeling
D007202:Indicators and Reagents; D008074:Lipoproteins; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL