Application of fluorescent antibody methods in the analysis of plaque samples.
Direct FA staining for S mutans serotypes may be performed on smears made from plaque or strain isolates and on colonies attached to agar plates of black membrane filters. Staining with single conjugates directed to S mutans serotypes (a to e) as well as with polyvalent a-, b-, d-, f-fluorescein label and c-, e-rhodamine label conjugates indicate that serotype c is the most common. This is in agreement with many reports by other investigators. Cross-reactions with many S mutans conjugates occurred with organisms resembling Lancefield groups C and G streptococci.
D000818:Animals; D003773:Dental Plaque / Q000382:microbiology*; D005455:Fluorescent Antibody Technique / Q000379:methods*; D006801:Humans; D012235:Rhodamines; D013194:Staining and Labeling; D013291:Streptococcus / Q000302:isolation & purification*; D013295:Streptococcus mutans / Q000145:classification / Q000276:immunology / Q000302:isolation & purification*
D012235:Rhodamines