title: Phagocytosis in human platelets: localization of acid phosphatase-positive phagosomes following latex uptake.
abstract: Incubation of human platelets in plasma containing a suspension of latex particles for 1-90 min resulted in progressive accumulation of particles in the open-channel system, followed by localization of latex in electron-opaque vacuoles. After 60 min, acid phosphatase was localized within latex-containing vacuoles. The periodate-alkaline-bismuth reaction intensely stained external membranes and membranes of the open-channel system. Membranes of latex-containing organelles were not stained. Latex phagocytosis was independent of both anticoagulant choice and aspirin effects. Our results indicate that the platelet can act as a true phagocyte, and we suggest that the phagocytic process is chronologically similar to that reported for polymorphonuclear leukocytes.
mesh_terms: D000135:Acid Phosphatase / Q000097:blood*; D001729:Bismuth; D001792:Blood Platelets / Q000502:physiology* / Q000648:ultrastructure; D002462:Cell Membrane / Q000648:ultrastructure; D006801:Humans; D066298:In Vitro Techniques; D007840:Latex; D008863:Microspheres; D010504:Periodic Acid; D010587:Phagocytosis; D013194:Staining and Labeling; D014617:Vacuoles / Q000201:enzymology
chemical_list: D007840:Latex; D010504:Periodic Acid; D000135:Acid Phosphatase; D001729:Bismuth