A review on methods of phospholipase A determination.
A short review is presented on methods to determine phospholipase A activities. On purpose, only those methods are covered which can, at least potentially, be used for assaying the low activities generally associated with intracellular phospholipases A. A selection was made of procedures which apply non-radioactive or radioactive substrates or chemically modified substrate analogs which can be used for spectrophotometric assays.
D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D003037:Cobalt Radioisotopes; D003300:Copper / Q000378:metabolism; D005230:Fatty Acids, Nonesterified; D005994:Glycerophosphates / Q000096:biosynthesis; D006863:Hydrogen-Ion Concentration; D008722:Methods; D010740:Phospholipases / Q000378:metabolism*; D010741:Phospholipases A / Q000378:metabolism*; D013053:Spectrophotometry; D013997:Time Factors
D003037:Cobalt Radioisotopes; D005230:Fatty Acids, Nonesterified; D005994:Glycerophosphates; D003300:Copper; D010740:Phospholipases; D010741:Phospholipases A