[Compact form of DNA in solution. XII. Double-stranded polyribonucleotide compacting in the presence of polyethylene glycol].
Double-stranded polyribonucleotides (a replicative form of phage f2 RNA--dsRNA and poly(A) poly(U), can adopt a compact from in solutions, containing NaCl and poly(ethylene glycol) (PEG). According to electron-microscopic observations dsRNA compact particles have the form of disks or doughnuts 200--400 A in diameter. X-ray diffraction patterns from dense slurries of dsRNA compact particles show a reflection at a spacing of 35 A, which is indicative of the existance of ordered regions in compact particles. The intense positive CD band, which is characteristic of dsRNA and poly(A) poly(U) compact particles, presumably results from the ordered regions in the particles. Heating of the solution leads to the disappearance of the intense positive CD band, probably as a result of the destruction of the ordered structure of compact particles. Heat or acid denatured dsRNA molecules as well as single-stranded molecules of ribosomal RNA also form large particles in PEG-containing solutions. However, X-ray diffraction patterns from these particles do not show the 35 A reflection and the specific positive band is not present in their CD spectra, which indicates that such particles lack ordered internal structure. It is suggested that similar mechanism of compactization of double-stranded polynucleotides (DNA and RNA) exist, and compact particles may be divided into two families (psi+ and psi-), differing by the secondary structure of double-stranded polynucleotides, which form the particles.
D001435:Bacteriophages / Q000032:analysis; D002942:Circular Dichroism; D006863:Hydrogen-Ion Concentration; D008854:Microscopy, Electron; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D011063:Poly A-U; D011092:Polyethylene Glycols; D012313:RNA; D012335:RNA, Ribosomal; D013057:Spectrum Analysis; D013696:Temperature; D014961:X-Ray Diffraction
D012335:RNA, Ribosomal; D011063:Poly A-U; D011092:Polyethylene Glycols; D012313:RNA
Kompaktnaia forma DNK v rastvore. XII. Kompaktizatsiia dvukhtsepochechnykh poliribonukleotidov v prisutstvii poliétilenglikolia.