A fluorimetric assay of the phosphoribosylation of 6-mercaptopurine in human blood cells.
A new fluorimetric method to assay HGPRT (hypoxanthine-guanine phosphoribosyl transferase, EC 2.4.2.8) from human red cell lysates using 6MP (6-mercaptopurine) as substrate is described. This assay is compared with an existing spectrophotometric method using hypoxanthine as substrate. Precision, sensitivity limits and kinetic differences of these assays are reported.
D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007041:Hypoxanthine Phosphoribosyltransferase / Q000097:blood*; D007700:Kinetics; D015122:Mercaptopurine / Q000097:blood*; D013050:Spectrometry, Fluorescence / Q000379:methods
D015122:Mercaptopurine; D007041:Hypoxanthine Phosphoribosyltransferase