An improved quantitative plaque assay for lymphocytes bearing antigen-specific cell receptors.
An improved method for the determination of the number of lymphoid cells bearing antigen-specific receptors is described. The method is based on the use of hapten-coupled bacteriophage (dinitrophenyl-T4) and detection of lytic plaques formed by the action of DNP-T4 on a target E. coli strain. The method is highly specific (up to 90% specific binding) and can be adapted for use with other antigenic determinants chemically attached to an active bacteriophage.
D000818:Animals; D001666:Binding Sites, Antibody; D002462:Cell Membrane / Q000276:immunology; D003090:Coliphages / Q000276:immunology; D000939:Epitopes; D006462:Hemolytic Plaque Technique; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009578:Nitrobenzenes / Q000276:immunology
D000939:Epitopes; D009578:Nitrobenzenes