Stearolyl-CoA desaturase: a control enzyme in hepatic lipogenesis.
1. Hepatocytes were isolated by perfusion of the liver with collagenase/salt solutions and incubated in culture after attachment to plastic culture dishes for periods up to 48 h. 2. The cells, when incubated in serum-free culture medium in the presence of insulin, showed enhanced stearolyl-CoA desaturase activity which was not observed when 50 muM cycloheximide was included. When insulin was omitted from the medium, the cells lost 80% of their original desaturase activity. 3. Cells isolated from animals fed 20% (w/w) sucrose for two weeks prior to sacrifice, showed high levels of fatty acid synthesis, stearolyl-CoA desaturase activity and triacylglycerol synthesis when compared with cells isolated from animals fed a corn oil supplemental diet. 4. The observations are discussed in terms of the influence of stearoyl-CoA desaturase activity on hepatic lipogenesis.
D000085:Acetates / Q000378:metabolism; D000818:Animals; D004040:Dietary Carbohydrates; D004041:Dietary Fats; D004790:Enzyme Induction; D044943:Fatty Acid Desaturases / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D066298:In Vitro Techniques; D007700:Kinetics; D008041:Linoleic Acids / Q000494:pharmacology; D008055:Lipids / Q000096:biosynthesis*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009821:Oils; D051381:Rats; D013229:Stearic Acids / Q000378:metabolism; D013230:Stearoyl-CoA Desaturase / Q000378:metabolism*; D013395:Sucrose / Q000494:pharmacology; D014280:Triglycerides / Q000378:metabolism
D000085:Acetates; D004040:Dietary Carbohydrates; D004041:Dietary Fats; D005227:Fatty Acids; D008041:Linoleic Acids; D008055:Lipids; D009821:Oils; D013229:Stearic Acids; D014280:Triglycerides; D013395:Sucrose; D044943:Fatty Acid Desaturases; D013230:Stearoyl-CoA Desaturase