title: Terminal dilution purification of adenovirus prototypes, and the antigenic relationship between types 4, 16 and 14.
abstract: Two terminal dilution purifications in HEK cells were performed with adenovirus prototypes of types 1 to 7, 12, 16, 19 and 9-15, and one terminal dilution with a freshly isolated type 8 strain. The antisera prepared against viruses after terminal dilution neutralized the original and the purified virus to the same extent, indicating the antigenic purity of the respective prototypes. Cross-reactions in neutralization with other types within the subgroup were also identical. Only the prototype of type 16 (Ch 79) showed a cross-neutralization with type 4, while four type 16 wild strains showed cross-neutralization with type 14, but none with type 4. The prototype (CH 79) is a "prime strain" in relation to the type 16 wild strains.
mesh_terms: D000256:Adenoviridae / Q000145:classification / Q000276:immunology / Q000302:isolation & purification*; D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D003429:Cross Reactions; D000939:Epitopes; D009500:Neutralization Tests; D012703:Serotyping
chemical_list: D000956:Antigens, Viral; D000939:Epitopes