Generation of cytotoxic lymphocytes in vitro: response of immune rat spleen cells to a syngeneic gross virus-induced lymphoma in mixed lymphocyte-tumor culture.
Spleen cells from W/Fu rats 4 to 6 weeks after immunization with syngeneic Gross virus-induced lymphoma (C58NT)D cells usually lack detectable activity in a short-term 51Cr release assay. The results presented here demonstrate that these spleen cells retain the capacity to generate significant proliferative and cytotoxic activity upon re-exposure to mitomycin C-treated (C58NT)D cells in vitro. Optimal conditions were defined in W/Fu rats for this secondary immune response in vitro to the (C58NT)D cells. The cytotoxic response was observed to be quantitative, reproducible, and specific. Optimal generation occurred 5 days after initiation of cultures with a 30:1 responding cell:stimulating cell ratio. In vitro generated cytotoxic cells inhibit tumor growth in vivo when administered as a mixture with tumor cells.
D000406:AKR murine leukemia virus; D000818:Animals; D002471:Cell Transformation, Neoplastic; D003601:Cytotoxicity Tests, Immunologic; D000939:Epitopes; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008223:Lymphoma / Q000209:etiology* / Q000276:immunology; D008937:Mitomycins / Q000494:pharmacology; D051381:Rats; D011914:Rats, Inbred BN; D011920:Rats, Inbred WF; D013154:Spleen / Q000276:immunology*; D013997:Time Factors; D014412:Tumor Virus Infections
D000939:Epitopes; D008937:Mitomycins