Dodecyl sulphate/polyacrylamide-gel electrophoresis at low pH values and low temperatures.
A simple method is described for dodecyl sulphate/polyacrylamide-gel electrophoresis of pH- and temperature-labile biological intermediates. The method is based on a catalyst system that works at temperatures of 2--4 degrees C and pH values of 2--4 and an appropriate buffer system containing Li+ or Tris [CH2OH--C(CH2OH)2--NH3+] instead of Na+. This system does not lead to the precipitation of 1% dodecyl sulphate.
D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008094:Lithium; D012967:Sodium Dodecyl Sulfate; D013696:Temperature; D014325:Tromethamine
D014325:Tromethamine; D012967:Sodium Dodecyl Sulfate; D008094:Lithium