A combined alkali extraction--ethidium bromide technique for the measurement of DNA in small pieces of tissue.
Alkaline solutions (0.1--0.5 N NaOH) at elevated temperatures can be used to extract DNA from small pieces of tissue. RNA is destroyed by the treatment. In tissues which have been previously exposed to tritiated thymidine, aliquots from the extracting solution can be used directly for the determination of DNA by ethidium bromide fluorescence and radioactivity in both DNA and the nucleotide pool.
D000818:Animals; D004247:DNA / Q000032:analysis* / Q000378:metabolism; D004996:Ethidium; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008722:Methods; D012313:RNA; D012867:Skin / Q000032:analysis / Q000378:metabolism; D013552:Swine; D013696:Temperature
D012313:RNA; D004247:DNA; D004996:Ethidium