Bovine leukemia virus: an exogenous RNA oncogenic virus.
Short-term cultures of bovine leukemic lymphocytes release virus particles with biochemical properties of RNA oncogenic viruses. These particles, tentatively called bovine leukemia virus (BLV), have a high molecular weight RNA-reverse transcriptase complex and a density of 1.155 g/ml in sucrose solutions. Molecular hybridizations between BLV/[3H]cDNA and several viral RNAs show that BLV is not related to Mason-Pfizer monkey virus, simian sarcoma associated virus, feline leukemia virus, or avian myeloblastosis virus. These results were confirmed by hybridization between BLV 70S RNA and [3H]cDNA synthesized in the various viruses tested. The high preference of BLV reverse transciptase for Mg++ as the divalent cation suggests that BLV might be an atypical mammalian leukemogenic "type C" virus. DNA-DNA hybridization studies using BLV [3H]cDNA as a probe strongly suggest that the DNA of bovine leukemic cells contains viral sequences that cannot be detected in normal bovine DNA.
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology; D002500:Centrifugation, Isopycnic; D001909:Leukemia Virus, Bovine / Q000145:classification* / Q000302:isolation & purification; D008228:Lymphoma, Non-Hodgkin / Q000382:microbiology / Q000662:veterinary; D009693:Nucleic Acid Hybridization; D012328:RNA Viruses / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*; D012190:Retroviridae / Q000032:analysis / Q000145:classification*
D012367:RNA, Viral; D012194:RNA-Directed DNA Polymerase