Mouse-human heterokaryon analysis with a 33258 Hoechst-Giemsa technique.
The bibenzimidazol derivative 33258 Hoechst can be used to distinguish microfluorometrically between mouse and human nuclei in heterokaryons.  This affords a quick and accurate alternative to autoradiography in the analysis of such heterokaryons.  The 33258 Hoechst fluorescence patterns can be converted after irradiation to a Giemsa rendition of the differential staining.
D000818:Animals; D001562:Benzimidazoles; D002460:Cell Line; D005456:Fluorescent Dyes; D005470:Fluorometry; D006367:HeLa Cells; D006801:Humans; D006822:Hybrid Cells / Q000032:analysis*; D007621:Karyotyping / Q000379:methods*; D007739:L Cells; D051379:Mice; D008809:Mice, Inbred C3H; D013194:Staining and Labeling
D001562:Benzimidazoles; D005456:Fluorescent Dyes