The transfer of J blood-group activity from J-containing bovine serum to J-negative erythrocytes.
The J blood-group activity of bovine serum is contained both in a lipid and in a nonlipid fraction. This is also true for calf serum. It demonstrated that the J determinant is transferred from a serum protein onto the erythrocyte membrane by incubation in vitro. Even though the donor of J activity is a lipid-free serum protein (probably a glycoprotein), the transferred J activity is detectable only in the lipid fraction of erythrocytes. Thus, the J determinant (probably a carbohydrate unit) must have been detached from a serum glycoprotein and transferred to a lipidic receptor (probably a glycosphingolipid) at the erythrocyte membrane. It is suggested than an enzyme system located in or at the erythrocyte membrane is responsible for the transfer of J substance. The transfer of J substance is inhibited by a polar lipid present in bovine serum.
D000375:Aging; D000818:Animals; D001789:Blood Group Antigens; D002417:Cattle; D000939:Epitopes; D004912:Erythrocytes / Q000378:metabolism*; D008055:Lipids / Q000097:blood; D013696:Temperature
D001789:Blood Group Antigens; D000939:Epitopes; D008055:Lipids