Studies on IgE in human intestinal fluids.
A fragment of IgE with molecular weight of about 40,000 was identified by radioimmunoassay in human small intestinal fluid after fractionation by gel filtration chromatography. Digestion of E myeloma protein PS by pooled intestinal fluid, trypsin or chymotrypsin yielded a degradation product of similar molecular weight that probably consisted principally of epsilon 1 determinant-containing fragments. These findings suggest that IgE is secreted into the intestinal lumen and degraded there by pancreatic proteolytic enzymes, producing an enzyme-resistant portion of the amino-terminal part of the Fc region.
D000906:Antibodies; D001667:Binding, Competitive; D002850:Chromatography, Gel; D002918:Chymotrypsin / Q000378:metabolism; D000939:Epitopes; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis*; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000276:immunology*; D009194:Myeloma Proteins / Q000276:immunology / Q000378:metabolism; D011863:Radioimmunoassay; D014357:Trypsin / Q000378:metabolism
D000906:Antibodies; D000939:Epitopes; D009194:Myeloma Proteins; D007073:Immunoglobulin E; D002918:Chymotrypsin; D014357:Trypsin