Quantitative analysis of methylguanidine and guanidine in physiologic fluids by high-performance liquid chromatography-fluorescence detection method.
A rapid and sensitive high-performance liquid chromatographic method has been developed for the quantitative analysis of methylguanidine and guanidine in physiological fluids. These quanidino compounds are separated on a 6 x 0.23 cm cation-exchange column with 0.5 M sodium hydroxide solution. The guanidino compounds are detected with a fluorometer, which monitors the fluorescent guanidine derivatives produced by the reaction of the eluted constituents with 9,10-phenanthrenequinone. Sensitivity to sub-nanomole levels of methylguanidine and guanidine is demonstrated. The method was successfully applied to physiological fluids such as serum and cerebrospinal fluid from uremic patients.
D002851:Chromatography, High Pressure Liquid; D006146:Guanidines / Q000032:analysis*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008722:Methods; D008760:Methylguanidine / Q000032:analysis*; D010616:Phenanthrenes; D011809:Quinones; D013050:Spectrometry, Fluorescence
D006146:Guanidines; D010616:Phenanthrenes; D011809:Quinones; D008760:Methylguanidine