Erythrocyte glucose metabolism in Duchenne muscular dystrophy.
We compared glucose metabolism by erythrocytes from patients with Duchenne muscular dystrophy and by erythrocytes from control individuals. There was a significant decrease in the rate of lactate production at pH 7.2 in the dystrophic group. When the pH of the incubation medium was changed to 8.0, we found that the increase in the rate of lactate production for the dystrophic group was significantly larger. We measured the concentrations of key glycolytic metabolites and adenine nucleotides and determined the values of the energy charge during these incubations. We also determined the concentrations of polyol pathway intermediates, the activities of the oxidative portion of the pentose phosphate pathway and the activities and kinetics of phosphofructokinase from both cell groups. There were no significant differences between groups for any of these variables.
D000227:Adenine Nucleotides / Q000097:blood; D000293:Adolescent; D000328:Adult; D001786:Blood Glucose / Q000378:metabolism*; D002648:Child; D004912:Erythrocytes / Q000378:metabolism*; D006019:Glycolysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007773:Lactates / Q000097:blood; D008297:Male; D009136:Muscular Dystrophies / Q000097:blood* / Q000235:genetics; D010428:Pentosephosphates / Q000097:blood; D010732:Phosphofructokinase-1 / Q000097:blood
D000227:Adenine Nucleotides; D001786:Blood Glucose; D007773:Lactates; D010428:Pentosephosphates; D010732:Phosphofructokinase-1