Reduction of nicotinamide adenine dinucleotides by sodium cyanoborohydride.
The relatively slow reduction of NAD+ and NADP+ by sodium cyanoborohydride leads to formation of the enzymically active form of NADH and NADPH. This reaction could be useful as a simple procedure to enzymically introduce a specific label into substrates when tritiated or deuterated cynanoborohydride is used for obtaining the reduced nicotinamide adenine dinucleotide.
D001894:Borohydrides; D003486:Cyanides; D007202:Indicators and Reagents; D007553:Isotope Labeling; D009243:NAD / Q000138:chemical synthesis*; D009249:NADP / Q000138:chemical synthesis*; D010084:Oxidation-Reduction
D001894:Borohydrides; D003486:Cyanides; D007202:Indicators and Reagents; D009243:NAD; D009249:NADP