A simple staining technique to demonstrate chromosomal DNA replication.
With the BrdU technique here described, Giemsa stained metaphases with either early or late replicational patterns can be obtained within 1 day after cell harvesting, showing a better resolution than 3H-thymidine autoradiography.
D001345:Autoradiography; D001399:Azure Stains; D001973:Bromodeoxyuridine / Q000378:metabolism; D002460:Cell Line; D004261:DNA Replication; D006801:Humans; D008938:Mitosis; D012730:Sex Chromosomes / Q000378:metabolism*; D013194:Staining and Labeling; D013936:Thymidine / Q000378:metabolism
D001399:Azure Stains; D001973:Bromodeoxyuridine; D013936:Thymidine