Multiple forms with glucose 6-phosphate dehydrogenase activity in Musca domestica L. as revealed by electrophoresis on cellulose acetate gel.
Single newly emerged males of Musca domestica, WHO strain, usually show five electrophoretic bands of glucose 6-phosphate dehydrogenase (G6PD) activity. Of these five molecular forms, designated with Roman numerals in order from the origin, we have considered the first three: these have been characterized with respect to their substrate and coenzyme specificity and to their sensitivity to some sulfhydryl inhibitors. The data show band III to be G6P specific, nicotinamide adenine dinucleotide phosphate dependent and to be a type I enzyme according to Kamada and Hori's classification. Bands I and II, on the other hand, show wide substrate specificity and low sensitivity to the sulfhydryl inhibitors assayed. In addition, in the absence of an exogenous substrate and in the presence of nicotinamide adenine dinucleotide as a coenzyme, fairly weak bands, which can be ascribed to the so called "nothing dehydrogenase" effect, are seen in the position I and II. Nevertheless, the data reported do not allow a clear definition of the enzymatic type corresponding to bands I and II of G6PD activity.
D000818:Animals; D002729:Chloromercuribenzoates / Q000494:pharmacology; D004588:Electrophoresis, Cellulose Acetate; D005033:Ethylmaleimide / Q000494:pharmacology; D005690:Galactose / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis / Q000378:metabolism*; D006600:Hexosephosphates / Q000378:metabolism; D006793:Houseflies / Q000201:enzymology*; D008297:Male; D009243:NAD / Q000494:pharmacology; D009249:NADP / Q000494:pharmacology; D012266:Ribose / Q000378:metabolism
D002729:Chloromercuribenzoates; D006600:Hexosephosphates; D009243:NAD; D009249:NADP; D012266:Ribose; D005954:Glucosephosphate Dehydrogenase; D005033:Ethylmaleimide; D005690:Galactose