title: In vitro effect of dopamine and pimozide on human chorionic somatomammotropin (hCS) secretion.
abstract: In human placental explants cultured in vitro, dopamine inhibited human chorionic somatomammotropin (hCS) secretion into the culture media. In the control flasks, the level of hCS secretion was 130.5 +/- 7.8 micrograms/g tissue (n = 6). When 1 mM dopamine was added, hCS levels decreased to 80.2 +/- 11.5 micrograms/g tissue (P less than 0.01). Dopamine (5 and 10 mM) further lowered hCS levels. In contrast, 1 mM pimozide enhanced hCS secretion by 2-fold as compared to control levels (248.2 +/- 44.8 vs. 130.5 +/- 7.8, P less than 0.02). The simultaneous addition of dopamine did not alter the stimulatory effect of pimozide on hCS secretion. In separate experiments, arginine (1 and 5 mM) and somatostatin (1 microgram/ml culture media) did not alter hCS secretion from placental explants. These results suggest that hCS secretion is modulated by dopaminergic receptors.
mesh_terms: D001120:Arginine / Q000494:pharmacology; D004298:Dopamine / Q000494:pharmacology*; D005260:Female; D005947:Glucose / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D009924:Organ Culture Techniques; D010868:Pimozide / Q000494:pharmacology*; D010920:Placenta / Q000187:drug effects / Q000378:metabolism*; D010928:Placental Lactogen / Q000378:metabolism*; D011247:Pregnancy; D013004:Somatostatin / Q000494:pharmacology
chemical_list: D010868:Pimozide; D013004:Somatostatin; D010928:Placental Lactogen; D001120:Arginine; D005947:Glucose; D004298:Dopamine