Affinity of human leukocyte interferon for polyribonucleotides.
Human leukocyte interferon (HL-IF)binds to AGPOLY(A)TM, AGPOLY(U)TM and AGPOLY(I)TM. The bound interferon could be displaced from all three polyribonucleotides by including sodium chloride in the eluant. The nature of interaction of HL-IF with polyribonucleotides is electrostatic and not hydrophobic since its binding was not prevented in the presence of 50% ethylene glycol. The binding of HL-IF on AGPOLY(I)TM is stronger at lower pH since an increase in ionic strength is required to displace it.
D001665:Binding Sites; D002846:Chromatography, Affinity; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007372:Interferons / Q000097:blood* / Q000302:isolation & purification; D007962:Leukocytes / Q000276:immunology*; D009522:Newcastle disease virus; D011061:Poly A; D011069:Poly I; D011072:Poly U; D011131:Polyribonucleotides; D012685:Sepharose
D011131:Polyribonucleotides; D011061:Poly A; D011069:Poly I; D011072:Poly U; D007372:Interferons; D012685:Sepharose