Relevance of three-dimensional reconstructions of stain distributions for structural analysis of biomolecules.
The relevance of a three-dimensional model of a protein molecule is discussed in the context of artefacts which inevitably occur during the preparation and imaging process. It is emphasized that even under optimistic assumptions, there-construction of a structure complementary to a given spatial stain distribution cannot, at resolutions below 10-15 A, be regarded as being identical with the real protein structure.
D005976:Glutaral; D008433:Mathematics; D008854:Microscopy, Electron / Q000379:methods; D008958:Models, Molecular; D008961:Models, Structural; D011485:Protein Binding; D011487:Protein Conformation; D013194:Staining and Labeling; D014961:X-Ray Diffraction / Q000379:methods
D005976:Glutaral