Purification of uricase from mammalian tissue.
A simple, rapid procedure for the purification of uricase from mammalian tissue is reported. The procedure is based on the precipitation of mammalian uricase under certain dialysis conditions, and on its low solubility near neutral pH. Exceptionally high yields of homogeneous enzyme are obtained.
D000818:Animals; D011232:Chemical Precipitation; D003956:Dialysis; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000201:enzymology*; D013552:Swine; D014503:Urate Oxidase / Q000302:isolation & purification*
D014503:Urate Oxidase