title: Regulation of acetohydroxy acid synthase in streptomycin-dependent Escherichia coli.
abstract: Growth of streptomycin-dependent mutants of Escherichia coli K-12 was insensitive to valine when dihydrostreptomycin was present in a nonlimiting concentration in glucose-salts medium. Acetohydroxy acid synthase was derepressed under these conditions, owing to relaxation of catabolite repression. Valine sensitivity and catabolite repression were restored when streptomycin-dependent E. coli K-12 mutants were grown with limiting dihydrostreptomycin. End product repression of acetohydroxy acid synthase under conditions of relaxed catabolite repression was effected by any two (or more) end products except the combination valine plus isoleucine, which caused derepression. Single end products had no detectable effect on acetohydroxy acid synthase formation.
mesh_terms: D000154:Aconitate Hydratase / Q000378:metabolism; D003470:Culture Media; D004096:Dihydrostreptomycin Sulfate / Q000378:metabolism; D004794:Enzyme Repression; D004926:Escherichia coli / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D005246:Feedback; D005649:Fumarate Hydratase / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D007532:Isoleucine / Q000494:pharmacology; D009154:Mutation; D007652:Oxo-Acid-Lyases / Q000378:metabolism*; D013307:Streptomycin / Q000378:metabolism*; D014633:Valine / Q000494:pharmacology
chemical_list: D003470:Culture Media; D007532:Isoleucine; D005954:Glucosephosphate Dehydrogenase; D007652:Oxo-Acid-Lyases; D005649:Fumarate Hydratase; D000154:Aconitate Hydratase; D014633:Valine; D005947:Glucose; D004096:Dihydrostreptomycin Sulfate; D013307:Streptomycin