Induction of T lymphocyte responses to a small molecular weight antigen. I. Failure to induce tolerance in azobenzenearsonate (ABA)-specific T cells in guinea pigs with an ABA conjugate of A copolymer of D-glutamic acid and D-lysine (D-GL).
2,4-Dinitrophenyl (DNP) coupled to the copolymer D-glutamic acid and D-lysine (D-GL) induces B cell tolerance but not T cell tolerance. This implies either a lack of DNP determinant recognition by T cells or a substantial difference in tolerance mechanisms for the two cell types. In the present study D-GL was conjugated with the well-defined determinant azobenzenearsonate (ABA) coupled to single amino acids shown here and previously by others to trigger effectively T lymphocytes. The experiments presented here demonstrate that these ABA conjugates of D-GL, although capable of diminishing anti-ABA antibody production, completely fail to render ABA-specific T lymphocytes tolerant thus drawing us to conclude that there are significant operational differences in the mechanisms of tolerance induction in T and B lymphocytes, respectively.
D000818:Animals; D000941:Antigens / Q000008:administration & dosage; D001391:Azo Compounds / Q000276:immunology*; D000939:Epitopes; D005971:Glutamates / Q000276:immunology*; D006168:Guinea Pigs; D006968:Hypersensitivity, Delayed / Q000276:immunology; D007108:Immune Tolerance / Q000187:drug effects*; D007074:Immunoglobulin G / Q000378:metabolism; D007271:Injections, Intradermal; D007328:Insulin / Q000378:metabolism; D008239:Lysine / Q000276:immunology*; D008297:Male; D008970:Molecular Weight; D011487:Protein Conformation; D012709:Serum Albumin / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000378:metabolism; D012882:Skin Tests; D013601:T-Lymphocytes / Q000276:immunology*; D014439:Tyramine / Q000378:metabolism; D014443:Tyrosine / Q000378:metabolism
D000941:Antigens; D001391:Azo Compounds; D000939:Epitopes; D005971:Glutamates; D007074:Immunoglobulin G; D007328:Insulin; D012709:Serum Albumin; D012710:Serum Albumin, Bovine; D014443:Tyrosine; D008239:Lysine; D014439:Tyramine