Influence of cultivation conditions on the production of staphylocoagulase by Staphylococcus aureus 104.
High yields of staphylocoagulase from Staphylococcus aureus 104 were obtained in a simple salts medium supplement with glycerol, casein hydrolysate and three vitamins. Conditions of oxygen-limitation (dissolved oxygen concentration less than 2%), a pH of 7.4, a temperature of 35 degrees C and a 1 in 10 inoculum of overnight culture were required for optimal yields of staphylocoagulase.
D002364:Caseins / Q000378:metabolism; D003030:Coagulase / Q000096:biosynthesis*; D003470:Culture Media; D005990:Glycerol / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D013211:Staphylococcus aureus / Q000201:enzymology*; D013696:Temperature; D014815:Vitamins / Q000378:metabolism
D002364:Caseins; D003030:Coagulase; D003470:Culture Media; D014815:Vitamins; D005990:Glycerol