title: The vaccinia virus haemagglutinin: enzyme sensitivity and some physiocochemical properties.
abstract: Purified vaccina virus haemagglutinin (VHA) was found to be sensitive to trypsin both with regard to haemagglutinating capacity and antigenic property. Phospholipase C and lipase had no effect on the haemagglutination (HA). Both the HA and the antigenic property thus depend on the integrity of the protein part. The loss of HA after treatment with trypsin may be due to breakdown of an essential protein or to secondary rearrangement of the whole structure, including the lipid part. Cells infected with an HA negative mutant of vaccinia virus also lacked the antigenic part of VHA. The sedimentation constant of VHA from HeLa cells was about 50.
mesh_terms: D000939:Epitopes; D006389:Hemagglutinins, Viral / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008049:Lipase; D010504:Periodic Acid; D010740:Phospholipases; D013696:Temperature; D014357:Trypsin; D014461:Ultracentrifugation; D014616:Vaccinia virus / Q000276:immunology*
chemical_list: D000939:Epitopes; D006389:Hemagglutinins, Viral; D010504:Periodic Acid; D010740:Phospholipases; D008049:Lipase; D014357:Trypsin