[Studies on lytic enzyme activities of bacteria and streptomycetes against Candida guilliermondii after partial purification with ammonium sulfate from shaking- and fermentation cultures (author's transl)].
Two bacteria and two streptomycetes strains, capable to degrade cell walls of Candida guilliermondii, were isolated from soils. Growth and enzyme production of these strains were investigated on synthetic and complex media in shaking culture and under fermentation conditions. The isolated microorganisms possessed lytic enzyme activities against living and autoclaved yeast cells. The lytic enzyme complex of the culture medium of microorganisms was partially purified by ammonium sulfate precipitation. After this purification step the lytic enzyme activity of the bacteria was higher that of the streptomycetes towards the control. The lytic enzyme activity of the bacteria was thermostable (30 min, 80 degrees C), that of streptomycetes thermolable (inactive - greater than 40 degrees C). The pH-optimum of the bacteria enzymes was in the range of 5.0-7.5, that of the streptomycetes 5.0-8.5.
D001419:Bacteria / Q000201:enzymology*; D002175:Candida / Q000378:metabolism*; D002473:Cell Wall / Q000378:metabolism; D011232:Chemical Precipitation; D003470:Culture Media; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D006867:Hydrolases / Q000302:isolation & purification / Q000378:metabolism*; D012988:Soil Microbiology; D013306:Streptomycetaceae / Q000201:enzymology*
D003470:Culture Media; D006867:Hydrolases
Untersuchungen zur lytischen Aktivität von Bakterien- und Streptomyceten-Stämmen gegen Candida guilliermondii nach teilweiser Reinigung mit (NH4)2SO4 aus Schüttel- und Fermenterkultur.