[Cooperative sorbtion  of trypaflavin by glycerinized muscle fibers. I. Adsorption isotherm and changes in the fluorescence polarization of the sorbed dye].
The equilibrium binding of a fluorescent cationic dye Tripaflavine (TPF) by frog's glicerinated mucle fibres is described by a S-shaped isotherm: the Langmuir sorption at low dye concentrations in the medium (0.84-10-5--3.38-10-5 M) is followed by the cooperative binding with the increase of concentration (8.45-10-5--338.0-10-5 M). The appearance of new TPF binding centres, alteration in fluorescence anisotropy of sorbed dye as well as inactivation of myosine ATPase of model fibres at high concentrations indicate that the cooperative phase of sorption is characterized by changes in the structure of contractile proteins.
D000167:Acriflavine / Q000378:metabolism; D000818:Animals; D009132:Muscles / Q000378:metabolism*; D013194:Staining and Labeling
D000167:Acriflavine
Kooperativnaia sorbtsiia tripaflavina glitserinizirovannymi myshechnymi voloknami. I. Izoterma adsorbtsii i izmenenie poliarizatsii fluorestsentsii sorbirovannogo krasitelia