The influence of fixation on staining of glycosaminoglycans in glial cells.
Cytoplasmic staining of glial cells by Alcian blue in 0.40 M magnesium chloride can be demonstrated in sections of brain tissue fixed in formol-acetic acid and in Bouin's solution. Phosphate-buffered aldehyde fixatives, whether at neutral or low pH, fail to preserve stainable material.
D000447:Aldehydes; D000818:Animals; D001923:Brain Chemistry; D004396:Coloring Agents; D005260:Female; D006025:Glycosaminoglycans / Q000032:analysis*; D006651:Histocytochemistry; D006652:Histological Techniques; D007211:Indoles; D008297:Male; D009457:Neuroglia / Q000032:analysis*; D051381:Rats; D013194:Staining and Labeling
D000447:Aldehydes; D004396:Coloring Agents; D006025:Glycosaminoglycans; D007211:Indoles