[Preparation of iduronate sulfatase from the human placenta].
The preparation of the enzyme iduronate sulfatase from human placenta has been undertaken. The substrate O-(alpha-L-idopyranosyluronic acid 2-sulfate) (1 leads to 4)-2,5-anhydro-D-[3H]mannitol 6-sulfate was used to measure the enzymatic activity. The enzyme shows a pH optimum of 4.0 in 0.1 M sodium formiate or acetate buffer. Chromatography on DE-52 gives a 5.4 fold purification. The enzyme is inhibited by NaCl or KCl: in 20 mM salt the reaction rate was only 63% and 34% respectively. Inhibition by salt can be removed by extensive dialysis after the chromatographic step.
D006801:Humans; D006863:Hydrogen-Ion Concentration; D007066:Iduronate Sulfatase / Q000032:analysis / Q000302:isolation & purification*; D010920:Placenta / Q000201:enzymology*; D011189:Potassium Chloride / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D013429:Sulfatases / Q000302:isolation & purification*
D012965:Sodium Chloride; D011189:Potassium Chloride; D013429:Sulfatases; D007066:Iduronate Sulfatase
Preparazione di iduronato solfatasi da placenta umana.