Periodic acid-Schiff-light green stain to detect glomerular protein deposits by routine light microscopy.
A simple, easily reproducible periodic acid-Schiff-light green stain (PAS-LG) for the detection of glomerular protein deposits by routine light microscopy is described. The deposits are selectively stained a deep blue and contrast sharply with the staining of adjacent glomerular structures. Correlation with immunofluorescent and electron microscopy has shown that it is possible with this stain to categorize accurately a large variety of glomerular lesions by light microscopy alone.
D000936:Antigen-Antibody Complex; D001485:Basement Membrane / Q000276:immunology / Q000473:pathology; D002466:Cell Nucleolus / Q000648:ultrastructure; D002467:Cell Nucleus / Q000648:ultrastructure; D003165:Complement System Proteins / Q000032:analysis; D003593:Cytoplasm / Q000648:ultrastructure; D004912:Erythrocytes / Q000473:pathology; D005337:Fibrin; D005455:Fluorescent Antibody Technique; D005921:Glomerulonephritis / Q000276:immunology / Q000473:pathology; D006801:Humans; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007202:Indicators and Reagents; D007674:Kidney Diseases / Q000276:immunology / Q000473:pathology; D007678:Kidney Glomerulus / Q000276:immunology / Q000473:pathology*; D007684:Kidney Tubules / Q000473:pathology; D008854:Microscopy, Electron; D010504:Periodic Acid; D011506:Proteins / Q000032:analysis*; D013194:Staining and Labeling / Q000379:methods*
D000936:Antigen-Antibody Complex; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007202:Indicators and Reagents; D011506:Proteins; D010504:Periodic Acid; D005337:Fibrin; D003165:Complement System Proteins