A combination of sister chromatid differential staining and giemsa banding.
We report a procedure for combining sister chromatid differential staining and G banding in the same metaphase plate. Mammalian cells in culture are grown in medium containing 5-bromodeoxyuridine for two cell cycles, and conventional air-dried preparations are made. The slides are treated with a trypsin or a urea solution the same way as for regular G banding. This method is simple and fast and provides additional information for cytogeneticists.
D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D002842:Chromatids / Q000648:ultrastructure*; D006224:Cricetinae; D006801:Humans; D006868:Hydrolysis; D051379:Mice; D008856:Microscopy, Fluorescence; D008938:Mitosis; D013194:Staining and Labeling; D014357:Trypsin
D014357:Trypsin; D001973:Bromodeoxyuridine