Prostaglandins and asthma: The use of blood components for metabolic studies.
Experiments were carried out to study a possible role of prostaglandins (PGs) in the pathogenesis of human bronchial asthma. Leukocyte, plasma, and serum of ambulatory asthmatic as well as nonasthmatic individuals were used. PGF was measured by competitive radioimmunoassay with anti-PGF2alpha antibody. It was found that PGF was spontaneously released from the leukocytes of both asthmatic and nonasthmatic individuals. The PGF release as studied with the cells of asthmatic individuals was enhanced by the additions of arachidonic acid and/or dibutyryl cyclic AMP. Antigenic challenge of sensitive cells failed to show any general effect on the amount of PGF released. The amount of spontaneously released PGF had no correlation with the amount of maximal antigenic histamine release. Preincubation of the cells with arachidonic acid had no effect on subsequent antigenic histamine release. Plasma PGF levels in nonasthmatic and in asthmatic individuals were not significantly different and both were considerably lower than the serum levels. The mean serum PGF level in the asthmatic group was higher than in the nonasthmatic, indicating probably a greater release of PGF from blood cells.
D000937:Antigen-Antibody Reactions; D001095:Arachidonic Acids / Q000494:pharmacology; D001249:Asthma / Q000276:immunology* / Q000378:metabolism; D003994:Bucladesine / Q000494:pharmacology; D005260:Female; D006636:Histamine Release / Q000187:drug effects; D006801:Humans; D007962:Leukocytes / Q000378:metabolism; D008297:Male; D011460:Prostaglandins F / Q000097:blood* / Q000378:metabolism; D013997:Time Factors
D001095:Arachidonic Acids; D011460:Prostaglandins F; D003994:Bucladesine