title: Electron microscopic autoradiography of 35SO4-labelled material closely associated with collagen fibrils in mammalian synovium and ear cartilage.
abstract: Electron microscopic autoradiography of connective tissue obtained from mice and rabbits previously injected with 35SO4 indicated that sulphated proteoglycans are localized on collagen fibrils. Ruthenium Red-positive transverse belts surrounding fibrils near the a-bands were heavily labelled, but fine lateral filaments of Ruthenium Red-positive material were not. These filaments, which interconnect collagen fibrils in a variety of connective tissues may represent linear aggregations of hyaluronic acid, glycoproteins and non-sulphated or long-lived sulphated proteoglycans.
mesh_terms: D000818:Animals; D002356:Cartilage / Q000032:analysis* / Q000648:ultrastructure; D003094:Collagen / Q000032:analysis*; D004423:Ear; D005260:Female; D006023:Glycoproteins / Q000032:analysis; D006820:Hyaluronic Acid / Q000032:analysis; D051379:Mice; D008854:Microscopy, Electron; D011817:Rabbits; D013194:Staining and Labeling; D013464:Sulfuric Acids / Q000032:analysis* / Q000097:blood; D013583:Synovial Membrane / Q000032:analysis* / Q000648:ultrastructure
chemical_list: D006023:Glycoproteins; D013464:Sulfuric Acids; D006820:Hyaluronic Acid; D003094:Collagen