Elimination of precipitates in oil red O fat stain by adding dextrin.
Addition of dextrin in the final 60% isopropanol of the Lillie-Ashburn super-saturated oil red O isopropanol technic moderately intensified the stain and decreased our required staining interval. Precipitates were decreased and the diluted solution remained usable into the second week. A saturated 60% isopropanol oil red O solution contained 33 mg/100 ml. Without dextrin the fresh supersaturated solution contains 40 mg, after 3 days 25 mg. With dextrin the fresh solution contained 130 mg dye, the 10-day-old one 100 mg/100 ml.
D000433:1-Propanol; D001391:Azo Compounds; D003912:Dextrins / Q000494:pharmacology*; D008055:Lipids / Q000032:analysis*; D009284:Naphthols; D011134:Polysaccharides / Q000494:pharmacology*; D013194:Staining and Labeling
D001391:Azo Compounds; D003912:Dextrins; D008055:Lipids; D009284:Naphthols; D011134:Polysaccharides; D000433:1-Propanol