Studies on the glycosidases of semen: purification and properties of alpha-D-mannopyranosidase from goat seminal plasma.
Alpha D-mannosidase activity in goat semen was observed to be distributed in sperm and seminal plasma. In sperm the enzyme, present in soluble and bound forms, was located within the acrosome. The bound enzyme was associated with the denuded sperm. Seminal plasma alpha-mannosidase was purified 100-fold and the final preparation was shown to be homogeneous by polyacrylamide and SDS gel electrophoresis and on isoelectric focusing. The molecular weight of the enzyme, determined by gel filtration and disc electrophoresis in the presence of SDS, was 220,000. The isoelectric pH was 7.42 and the amino acid composition is reported. alpha-Mannosidase catalyzed the hydrolysis of both synthetic and natural substrates. The Km of p-nitrophenyl alpha-D-mannoside and alpha-methyl D-mannoside were 0.695 mM and 71.9 mM at pH 4.0, the optimum pH. The natural substrates were hydrolysed to varying degrees. Zn2+ was not essential though it activated the enzyme activity over longer incubations. The enzyme was observed to be more stable at wider pH range in the presence of Zn2+ than in its absence. EDTA which did not affect the enzyme activity has effect on enzyme stability similar to Zn.2+ Seminal alpha-mannosidase is not a zinc metalloenzyme but is activated by Zn2+.
D000177:Acrosome / Q000201:enzymology; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002458:Cell Fractionation; D055598:Chemical Phenomena; D002621:Chemistry; D004355:Drug Stability; D006041:Goats / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008297:Male; D008361:Mannosidases / Q000302:isolation & purification* / Q000378:metabolism; D012661:Semen / Q000201:enzymology*; D013094:Spermatozoa / Q000201:enzymology*
D000596:Amino Acids; D008361:Mannosidases