H-2 private specificity of in vivo-sensitized lymphocytes studied by adsorption on fibroblast monolayers.
Spleen cells or thymocytes immune to one H-2 genotype are cytotoxic to allogeneic target cells of a third-party genotype, provided the target shares private specificities determined by either the H-2K or the H-2D locus of the H-2 complex with the H-2 genotype used for sensitization in vivo. The present results on elimination of cytotoxicity to specificities determined by one of the H-2 loci after adsorption of the immune lymphoid cells onto fibroblasts bearing this specificity suggest a state of diversity among effector lymphocytes reactive to the antigens determined by either the H-2K or the H-2D locus of the H-2 complex. Thymus cells activated in vivo were less specifically adsorbable than spleen cells of tumor-allografted mice.
D000327:Adsorption; D000818:Animals; D000937:Antigen-Antibody Reactions; D003601:Cytotoxicity Tests, Immunologic; D000939:Epitopes; D005347:Fibroblasts / Q000276:immunology*; D005838:Genotype; D006649:Histocompatibility Antigens; D007114:Immunization; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D008325:Mammary Neoplasms, Experimental / Q000276:immunology; D051379:Mice; D008805:Mice, Inbred A; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D009368:Neoplasm Transplantation; D012513:Sarcoma, Experimental / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D014184:Transplantation, Homologous
D000939:Epitopes; D006649:Histocompatibility Antigens