Fluorescent demonstration of basement membranes with hydroxy-naphthoic acid hydrazide.
The hydroxy-naphthoic acid hydrazide (HNAH) procedure can be employed not only for selective fluorochroming of side chain carboxyl groups of proteins, but also for demonstration of certain exoplasmic structures---particularly basement membranes. The demonstration of basement membranes by the HNAH procedure is likely related to the relatively high levels of available side chain carboxyl groups in the noncollagen glycoproteins of basement membranes.
D000818:Animals; D001485:Basement Membrane / Q000032:analysis*; D002264:Carboxylic Acids; D005456:Fluorescent Dyes; D006651:Histocytochemistry; D007668:Kidney / Q000166:cytology; D009284:Naphthols; D011894:Rana pipiens; D013194:Staining and Labeling
D002264:Carboxylic Acids; D005456:Fluorescent Dyes; D009284:Naphthols