Isolation and characterization of an antigen of the bovine C-type virus.
By means of gel filtration and isoelectric focusing, an antigen of the bovine C-type leukemia virus was isolated in a highly purified form from extracts of infected cells. The antigen has a molecular weight of approximately 25,000 daltons and an isoelectric point of 6.4 to 6.6. In immunodiffusion experiments, the antigen forms a line of identity with an antigen extracted from highly purified bovine C-type leukemia virus by treatment with ether or Triton X-100. As determined by immunodiffusion analyses, the bovine C-type leukemia virus antigen does not have antigenic determinants in common with the murine or feline leukemia viruses, the foamy-like bovine syncytia virus, or the Mason Pfizer monkey virus.
D000956:Antigens, Viral / Q000302:isolation & purification*; D002478:Cells, Cultured; D002850:Chromatography, Gel; D003429:Cross Reactions; D000939:Epitopes; D005779:Immunodiffusion; D007525:Isoelectric Focusing; D007553:Isotope Labeling; D001909:Leukemia Virus, Bovine / Q000276:immunology*; D005256:Leukemia Virus, Feline / Q000276:immunology; D008970:Molecular Weight; D009858:Oncogenic Viruses / Q000276:immunology; D012190:Retroviridae / Q000276:immunology*; D014764:Viral Proteins / Q000032:analysis
D000956:Antigens, Viral; D000939:Epitopes; D014764:Viral Proteins