[Cytotoxic effects of a leukocidin from Staphylococcus aureus (author's transl)].
In an attempt to improve the assay of leukocidin from Staphylococcus aureus a combined vital fluorochrome staining procedure with acridine organe and ethidium bromide was developed. It proved to be more suitable for the demonstration of leukocidic effects on leukocytes than phasecontrastmicroscopy. Damaged leukocytes fluoresced distinctly red and undamaged leukocytes green. With the fluorochrome-method leukocidic changes became also evident in some lymphocytes. These were not clearly demonstrated by phasecontrastmicroscopy.
D000166:Acridines; D000818:Animals; D001681:Biological Assay; D002417:Cattle; D004996:Ethidium; D005456:Fluorescent Dyes; D006098:Granulocytes / Q000187:drug effects; D007956:Leukocidins / Q000032:analysis / Q000494:pharmacology*; D007962:Leukocytes / Q000187:drug effects*; D008214:Lymphocytes / Q000187:drug effects; D008856:Microscopy, Fluorescence; D008858:Microscopy, Phase-Contrast; D012756:Sheep; D013194:Staining and Labeling; D013211:Staphylococcus aureus
D000166:Acridines; D005456:Fluorescent Dyes; D007956:Leukocidins; D004996:Ethidium
Cytotoxische Wirkungen eines Leukozidins von Staphylococcus aureus