Blocking antigen-antibody complexes on the T-lymphocye surface identified with defined protein antigens. II. Lymphocyte activation during the in vitro response.
Antibody-forming cells against DNP (DNP-AFC) appear in nonantigen-stimulated cultures of spleen cells from mice which were boosted with antigen DNP-CGG or TNP-KLH in saline several weeks before the vitro cultures were started. The similar kinetics of the appearance of DNP-AFC in antigen-stimulated and non-antigen-stimulated cultures suggests that new B cells were triggered during the in vitro culture. The data supporting the concept of antigen being retained in the body for long periods of time in the form of antigen-antibody complexes on the T-cell surface is discussed.
D000818:Animals; D000917:Antibody Formation; D000921:Antibody-Producing Cells; D000936:Antigen-Antibody Complex; D000941:Antigens / Q000008:administration & dosage; D001402:B-Lymphocytes / Q000276:immunology; D002473:Cell Wall / Q000276:immunology; D002478:Cells, Cultured; D004140:Dinitrophenols / Q000276:immunology; D007106:Immune Sera; D051379:Mice; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014357:Trypsin; D005719:gamma-Globulins
D000936:Antigen-Antibody Complex; D000941:Antigens; D004140:Dinitrophenols; D007106:Immune Sera; D005719:gamma-Globulins; D014357:Trypsin