Quantitation of specific antibodies in the aqueous humor.
Six normal rabbits were injected intravitreally with bovine gamma-globulin (BGG). Quantitation of immunoglobulins A, M and G, in the aqueous and in the serum, was performed by the single radial immunodiffusion following the method described by MANCINI et. al. Concentration of anti-BGG antibodies was determined by the reversed Mancini method. The antibody quotient was calculated; it reached to 8 and 5.96 in the aqueous of the injected eyes 10 days after intravitreal injection, 22.69 and 24 days, 22.52 after 28 days and 14.93 after 31 days.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D001082:Aqueous Humor / Q000276:immunology*; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D011817:Rabbits; D014605:Uveitis / Q000276:immunology; D005719:gamma-Globulins
D000906:Antibodies; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D005719:gamma-Globulins