Physical properties of human serum hexosaminidases A and B: studies in normal and cancer patients.
The major forms of human serum hexosaminidases A and B (Hex A and Hex B) were isolated from normal subjects and cancer patients using DEAE-cellulose. In normal serum, Hex A was heat-labile and had an apparent KM of 1.13 mM and Vmax of 0.51 mumol/ml/h; Hex B was heat-stable and had an apparent KM of 0.85 mM and Vmax of 0.22 mumol/ml/h. Both forms had the same pH optimum, at 4.3. Hex A and Hex B from the sera of cancer patients resembled their normal counterparts in heat stability, pH optimum, and apparent KM (1.07 mM for Hex A, 0.88 mM for Hex B). In contrast, the Vmax values for the cancer sera isozymes were greater than those of normal sera (0.70 and 0.40 mumol/ml/h for Hex A and Hex B, respectively).
D006596:Hexosaminidases / Q000097:blood / Q000302:isolation & purification*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008722:Methods; D009369:Neoplasms / Q000097:blood / Q000201:enzymology*; D009596:Nitrophenols; D013696:Temperature; D013997:Time Factors
D009596:Nitrophenols; D006596:Hexosaminidases