title: Role of vitamin B12 and enzymes related to methylmalonyl-CoA mutase in a methanol-utilizing bacterium, Protaminobacter ruber.
abstract: A methanol-utilizing bacterium, Protaminobacter ruber, required cobalt ion or vitamin B12 as its growth factor, which could be replaced by succinate among various additions to the cobalt-deficient medium. The presence of adenosylcobalamin (adenosyl-B12)-dependent methylmalonyl-coenzyme A (CoA) mutase was demonstrated in the cell-free extracts of P. ruber. The specific activity of this mutase was not only fairly high in comparison with that reported with other organisms but also detected at a similar level throughout the cultivation period. The cell-free extracts of P. ruber grown on non-C1 compounds as a sole carbon and energy source also had methylmalonyl-CoA mutase activity. Furthermore, the extracts of this microorganism catalyzed the reactions from propionyl-CoA to succinyl-CoA and from alpha-ketoglutarate to alpha-hydroxyglutarate.
mesh_terms: D002474:Cell-Free System; D003035:Cobalt / Q000494:pharmacology; D007535:Isomerases / Q000378:metabolism*; D008765:Methylmalonyl-CoA Mutase / Q000378:metabolism*; D008772:Methylococcaceae / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D014805:Vitamin B 12 / Q000494:pharmacology*
chemical_list: D003035:Cobalt; D007535:Isomerases; D008765:Methylmalonyl-CoA Mutase; D014805:Vitamin B 12