title: [Method of determination of fat deposit in chickens].
abstract: The method described is based on the histochemical demonstration of fats with the use of Sudan stains and the clearing of tissues in an alkaline medium. It is employed to investigate whole birds, thick sections or individual organs to determine the morphotopographic localization of the fat depots. The procedure comprises the alcohol fixation of tissues, the staining of lipids with Sudan black, the clearing of tissues in a solution of potassium hydroxide, and the final mounting of the cleared materials in glycerine. This treatment results in that the sites of fat deposition assume a blue coloration, the bones are grey, and all remaining tissues and organs (if properly bled) are colorless and transparent. The method can also be successfully used with materials that have been preliminary fixed with formalin but their clearing has been considerably worse.
mesh_terms: D000818:Animals; D002645:Chickens; D005223:Fats / Q000032:analysis*; D008722:Methods; D013194:Staining and Labeling
chemical_list: D005223:Fats
vernacular_title: Metod za opredeliane na mastnite depa pri pileta