title: Energetics of the Na+-dependent transport of D-glucose in renal brush border membrane vesicles.
abstract: The energetics of the Na+-dependent transport of D-glucose into osmotically active membrane vesicles, derived from the brush borders of the rabbit renal proximal tubule, was studied by determining how alterations in the electrochemical potential of the membrane induced by anions, ionophores, and a proton conductor affect the uptake of the sugar. The imposition of a large NaCl gradient (medium is greater than vesicle) resulted in the transient uptake of D-glucose into brush border membranes against its concentration gradient. In the presence of Na+ salts of isethionate or sulfate, both relatively impermeable anions, there was no accumulation of D-glucose above the equilibrium value. With Na+ salts of two highly permeable lipophilic anions, NO3- and SCN-, the transient overshoot was enhanced relative to that with Cl-. With Na+ salts whose mode of membrane translocation is electroneutral, i.e. acetate, bicarbonate, and phosphate, no overshoot was found. These findings suggest that only anions which penetrate the brush border membrane and generate an electrochemical potential, negative on the inside, permit the uphill Na+-dependent transport of D-glucose.
mesh_terms: D000818:Animals; D000838:Anions; D001693:Biological Transport, Active; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D006096:Gramicidin / Q000494:pharmacology; D007687:Kidney Tubules, Proximal / Q000187:drug effects / Q000378:metabolism*; D008353:Mannitol / Q000378:metabolism; D008954:Models, Biological; D009550:Nigericin / Q000494:pharmacology; D009994:Osmolar Concentration; D011817:Rabbits; D012964:Sodium / Q000494:pharmacology*; D014634:Valinomycin / Q000494:pharmacology
chemical_list: D000838:Anions; D006096:Gramicidin; D014634:Valinomycin; D008353:Mannitol; D012964:Sodium; D005947:Glucose; D009550:Nigericin