Multiple in vitro mechanisms of tumor cytotoxicity demonstrated in the line-1 guinea pig hepatoma model.
The line-1 guinea pig hepatoma was used to study in vitro tumor cytotoxicity. Cytotoxicity was determined by measurement of the loss of tritiated thymidine-labeled target cells from culture vessels. With this technique, we demonstrated that significant tumor cytotoxicity was caused by lymphoid cells from tumor-immune guinea pigs, by cells from guinea pigs immunized against an antigen urelated to the tumor target, and by cell-free supernatants rich in lymphocyte mediators. Addition of normal peritoneal exudate cells enhanced the cytotoxic potential of a small number of highly purified immune lymphocytes, which suggested that recruitment of normal cells is an additional mechanism of tumor cell death in this system.
D000818:Animals; D000918:Antibody Specificity; D000951:Antigens, Neoplasm; D001202:Ascitic Fluid / Q000166:cytology; D001500:BCG Vaccine; D006528:Carcinoma, Hepatocellular / Q000276:immunology*; D002474:Cell-Free System; D003601:Cytotoxicity Tests, Immunologic; D006168:Guinea Pigs; D007111:Immunity, Cellular; D008113:Liver Neoplasms / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology; D009163:Mycobacterium bovis / Q000276:immunology; D009374:Neoplasms, Experimental / Q000276:immunology; D013154:Spleen / Q000276:immunology; D005719:gamma-Globulins
D000951:Antigens, Neoplasm; D001500:BCG Vaccine; D005719:gamma-Globulins