Characterization of a soluble glycolipid galactosyltransferase which occurs in bovine milk.
Bovine milk was found to contain, in soluble form, an enzyme which transfers galactose from UDPgalactose to glucosylceramide. This enzyme was partially purified by the same procedure used to isolate the galactosyltransferase of lactose synthetase. The partially purified enzyme required detergents for activity, had a pH optimum of 7.2--7.3 and required Mn2+. The apparent Km calculated for glucosylceramide was 1.33 . 10(-4) M. With glucosylceramide as acceptor the product of the reaction was identified as lactosylceramide by autoradiography on thin-layer chromatograms. Lactosylceramide was also an effective acceptor for the transferase reaction but neutral glycosphingolipids or gangliosides with terminal galactose of N-acetylgalactosamine residues were ineffective or poorly effective as acceptors. Addition of alpha-lactalbumin inhibited the transferase reaction.
D000818:Animals; D002417:Cattle; D003902:Detergents / Q000494:pharmacology; D005700:Galactosyltransferases / Q000302:isolation & purification / Q000378:metabolism*; D005963:Glucosylceramides / Q000378:metabolism; D006017:Glycolipids; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007768:Lactalbumin / Q000494:pharmacology; D007790:Lactosylceramides / Q000378:metabolism; D008345:Manganese / Q000494:pharmacology; D008892:Milk / Q000201:enzymology*; D014531:Uridine Diphosphate Galactose / Q000378:metabolism
D003902:Detergents; D005963:Glucosylceramides; D006017:Glycolipids; D007790:Lactosylceramides; D014531:Uridine Diphosphate Galactose; D008345:Manganese; D007768:Lactalbumin; D005700:Galactosyltransferases; C020820:glycolipid galactosyltransferase