title: Studies on dehydrogenases of the glucuronate-xylulose cycle in the livers of diabetic mice and rats.
abstract: In view of reports that accessory pathways of glucose oxidation are enhanced in the diabetic state, we have determined the levels of key enzymes of the glucuronate-xylulose cycle in the livers of diabetic mice and rats. Genetically diabetic mice (db/db) were found to have increased levels of two NADP-linked enzymes of this cycle [NADP-xylitol dehydrogenase and NADP-L-hexonate dehydrogenase (aldehyde reductase II)], whereas other NAD- and NADP-linked dehydrogenase activities of the pathway were not changed. On the other hand, the livers of streptozotocin-diabetic mice and rats showed normal levels of all these enzymes. In the course of this study, evidence was obtained for the presence in db/db mouse liver of low molecular weight material inhibitory for glucose 6-phosphate dehydrogenase. The use of these animal models in diabetes research is briefly discussed.
mesh_terms: D000375:Aging; D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D000818:Animals; D002237:Carbohydrate Dehydrogenases / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology* / Q000235:genetics; D005965:Glucuronates / Q000378:metabolism*; D008099:Liver / Q000201:enzymology* / Q000254:growth & development; D008297:Male; D051379:Mice; D009243:NAD; D009249:NADP; D010429:Pentoses / Q000378:metabolism*; D051381:Rats; D014996:Xylulose / Q000378:metabolism*
chemical_list: D005965:Glucuronates; D010429:Pentoses; D009243:NAD; D009249:NADP; D014996:Xylulose; D000429:Alcohol Oxidoreductases; D002237:Carbohydrate Dehydrogenases