Characterizations of acylagmatine amidohydrolase and carboxypeptidase from Fusarium anguioides.
Previously an enzyme, named acylagmatine amidohydrolase, hydrolyzing bleomycin B2 to bleomycinic acid and agmatine was found in the mycelia of Fusarium anguioides Sherbakoff.  In this work the enzyme was purified further, but not completely.  The crude enzyme preparation hydrolyzed various acylagmatines and also peptidyl arginine, but the latter activity could be separated from acylagmatine amidohydrolase activity by gel filtration on Sephadex G-100.  The enzyme was inhibited by PCMB and its molecular weight was estimated as 65,000 by gel filtration.  It showed substrate specificity with respect to the alkyl-chain length of the amine moiety.  The other hydrolase fraction with activity toward Bz-Gly-Arg was found to be of a sort of carboxypeptidase, which preferentially hydrolyzed peptides with arginine or lysine at the carboxyl terminus, including bradykinin, but liberated neutral amino acids as well from the terminus when the penultimate residue of the substrates was phenylalanine.  With Bz-Gly-Arg as substrate Fusarium carboxypeptidase was sensitive to chelating agents but not to diisopropyfluorophosphate, and its molecular weight was estimated to be 145,000.
D000376:Agmatine / Q000031:analogs & derivatives; D000581:Amidohydrolases / Q000037:antagonists & inhibitors / Q000302:isolation & purification* / Q000378:metabolism; D000596:Amino Acids / Q000032:analysis; D001761:Bleomycin / Q000378:metabolism; D001920:Bradykinin / Q000378:metabolism; D002268:Carboxypeptidases / Q000037:antagonists & inhibitors / Q000302:isolation & purification* / Q000378:metabolism; D002729:Chloromercuribenzoates; D002850:Chromatography, Gel; D005670:Fusarium / Q000201:enzymology*; D007531:Isoflurophate; D008970:Molecular Weight; D010649:Phenylalanine; D011506:Proteins / Q000378:metabolism
D000596:Amino Acids; D002729:Chloromercuribenzoates; D011506:Proteins; D001761:Bleomycin; D007531:Isoflurophate; D010649:Phenylalanine; D000376:Agmatine; D002268:Carboxypeptidases; D000581:Amidohydrolases; D001920:Bradykinin