The radioimmunoassay of steroid glucuronides. The oestrogen C-3 glucuronides as haptens.
Antisera were prepared against three related oestrogen ring-A glucuronides, oestrone 3-glucuronide, oestradiol 3-glucuronide and oestriol 3-glucuronide. The corresponding 6,7-3H-labelled conjugates were synthesized as radioligands and the cross-reactions of the antisera against ring-A oestrogen glucuronides and other steroid conjugates were examined. The specificity of the antiserum against oestriol 3-glucuronide was compared with that raised against oestriol 16alpha-glucuronide, and the measurement of the former conjugate in late-pregnancy urine is discussed.
D000918:Antibody Specificity; D003429:Cross Reactions; D000939:Epitopes; D004958:Estradiol / Q000276:immunology; D004967:Estrogens / Q000276:immunology*; D004970:Estrone / Q000276:immunology; D005965:Glucuronates / Q000276:immunology*; D006241:Haptens; D007106:Immune Sera; D011863:Radioimmunoassay; D012710:Serum Albumin, Bovine; D013997:Time Factors
D000939:Epitopes; D004967:Estrogens; D005965:Glucuronates; D006241:Haptens; D007106:Immune Sera; D012710:Serum Albumin, Bovine; D004970:Estrone; D004958:Estradiol