Continuous production of NADP by immobilized Brevibacterium ammoniagenes cells.
Whole cells of Brevibacterium ammoniagenes IAM 1645 having the polyphosphate NAD-kinase were successfully immobilized in a polyacrylamide gel lattice. The immobilized cells were activated by treatment with organic solvents or detergents. The pH optimum of the immobilized cells for the production of NADP was 7.0, and divalent metal ions were required to maintain the elevated activity of polyphosphate NAD-kinase. Highly pure NADP was continuously produced in high yield by the immobilized cell column. The half-life of this column was about eight days.
D001951:Brevibacterium / Q000166:cytology / Q000201:enzymology*; D004800:Enzymes, Immobilized / Q000378:metabolism; D008274:Magnesium / Q000494:pharmacology; D009243:NAD; D009249:NADP / Q000096:biosynthesis*; D010770:Phosphotransferases / Q000378:metabolism*; D011122:Polyphosphates; D013696:Temperature
D004800:Enzymes, Immobilized; D011122:Polyphosphates; D009243:NAD; D009249:NADP; D010770:Phosphotransferases; C019534:polyphosphate NAD-kinase; D008274:Magnesium