title: A dual, concentration-dependent absorption mechanism of linoleic acid by rat jejunum in vitro.
abstract: Linoleic acid absorption was studied using everted rat jejunal sacs. At low concentrations (42-1260 microM), the relationship between linoleic acid concentration and its absorption rate fitted best to a rectangular hyperbola. At high concentrations (2.5-4.2 mM) the relationship between the two parameters was linear. The separate additions of 2,4-dinitrophenol, cyanide, or azide, or decrease in the incubation temperature from 37 to 20 degrees C did not change the absorption rate of linoleic acid. Absorption rate of linoleic acid at low concentrations increased as the hydrogen ion and taurocholate concentrations were increased or as the unstirred water layer thickness was decreased. Linoleic acid absorption rate was decreased after the additions of lecithin, oleic, linolenic, and arachidonic acids or the substitution of taurocholate with the nonionic surfactant Pluronic F 68. These observations indicate that a concentration-dependent, dual mechanism of transport is operative in linoleic acid absorption. Facilitated diffusion is the predominant mechanism of absorption at low concentrations, while at high concentrations, simple diffusion is predominant. At low concentrations, the absorption rate of linoleic acid is influenced by the pH, surfactant type and concentration, the simultaneous presence of other polyunsaturated fatty acids, and the thickness of the unstirred water layer.
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007408:Intestinal Absorption / Q000187:drug effects; D007583:Jejunum / Q000378:metabolism*; D008041:Linoleic Acids / Q000378:metabolism*; D008297:Male; D010713:Phosphatidylcholines / Q000494:pharmacology; D051381:Rats; D013329:Structure-Activity Relationship; D013499:Surface Properties; D013501:Surface-Active Agents / Q000494:pharmacology; D013656:Taurocholic Acid / Q000494:pharmacology; D014475:Uncoupling Agents / Q000494:pharmacology
chemical_list: D005231:Fatty Acids, Unsaturated; D008041:Linoleic Acids; D010713:Phosphatidylcholines; D013501:Surface-Active Agents; D014475:Uncoupling Agents; D013656:Taurocholic Acid