[Detection of neoplastic cells in the bloodstream].
A study was made of the efficacy of trypan blue, acridine orange, tetracycline and oxytetracycline for detection of tumour cells injected into the blood stream of rats. The cells were identified in the mesenteric microvessels by intravital microscopy. Fluorescence of fluorochromized cells was observed in the blue-violet (lambda max = 400 nm) and ultra-violet (lambda max = 365 nm) irradiation of the fluorescent lamp and in the laser irradiation (lambda = 337 nm). The cells stained with acridine orange had a higher fluorescence intensity and a more distinct structure than those labelled with tetracyclines. Identification of cells with trypan blue was more difficult. The fluorescent method of determination is rather simple and permits to indentify tumour cells directly in the blood stream.
D000166:Acridines; D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D008113:Liver Neoplasms; D008856:Microscopy, Fluorescence; D009374:Neoplasms, Experimental / Q000473:pathology; D009360:Neoplastic Cells, Circulating; D010118:Oxytetracycline; D051381:Rats; D013194:Staining and Labeling; D013752:Tetracycline; D014343:Trypan Blue
D000166:Acridines; D013752:Tetracycline; D014343:Trypan Blue; D010118:Oxytetracycline
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