title: Comparison of G-, Q- and EM-banding patterns exhibited by the chromosome complement of the Indian muntjac, Muntiacus muntjak, with reference to nuclear DNA content and chromatin ultrastructure.
abstract: When the chromosomes of the Indian muntjac, Muntiacus muntjak, were compared following treatment with two presently used banding methods, trypsin-Giemsa (G) and quinacrine-hydrochloride (Q) with structural bands as seen in the electron microscope, definite correlations were observed with respect to the numbers and positions of individual bands. - Weights obtained for the individual chromosomes were: No. 1, 9.98 pg; No. 2, 4.10 pg; No. 3, 4.43 pg; No. 3-X, 5.05 pg; and Y, 0.55 pg. Average diameters and weights for individual fibers were 193 A and 8.74 times 10-16 g/micron, respectively, for stimulated metaphase chromosomes and 185 A and 8.73 times 10-16 g/micron, respectively, for unstimulated chromosomes. Fibers of interphase nuclei exhibited an average diameter of 191 A and a weight of 5.87 times 10-16 g/micron. - The total amound of nuclear DNA present in interphase nuclei was 3.88 pg.
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000032:analysis; D002843:Chromatin / Q000032:analysis*; D002875:Chromosomes / Q000032:analysis* / Q000648:ultrastructure; D004247:DNA / Q000032:analysis*; D003670:Deer; D003720:Densitometry; D007621:Karyotyping; D008856:Microscopy, Fluorescence; D010783:Photometry; D011796:Quinacrine; D013194:Staining and Labeling; D014357:Trypsin
chemical_list: D002843:Chromatin; D004247:DNA; D014357:Trypsin; D011796:Quinacrine