Detection and characterization of RNA tumor virus-specific DNA in cells.
RNA tumor virus-specific DNA in cells can be detected by its capacity to 1) alter the reassociation kinetics of labeled double-stranded product of viral RNA-directed DNA polymerase; 2) anneal single-stranded DNA (cDNA) synthesized by viral polymerase; or 3) hybridize labeled viral 70S (genomic) RNA. Duplexes formed with these procedures can be analyzed for fidelity of base pairing, and the integration of viral DNA into the host genome can be established with a simple but stringent technique. We illustrate this methodology as applied to detection of Rous sarcoma virus (RSV)-specific DNA in XC cells and of mouse mammary tumor virus (MMTV)-specific DNA in murine and human tissues.
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000032:analysis; D002460:Cell Line; D004277:DNA, Single-Stranded / Q000096:biosynthesis; D004279:DNA, Viral / Q000032:analysis*; D006801:Humans; D008324:Mammary Tumor Virus, Mouse / Q000032:analysis; D051379:Mice; D009693:Nucleic Acid Hybridization; D012328:RNA Viruses / Q000032:analysis*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D012513:Sarcoma, Experimental / Q000382:microbiology
D004277:DNA, Single-Stranded; D004279:DNA, Viral; D012194:RNA-Directed DNA Polymerase