The effect of fibrin deposition on the sensitivity of the continuous monitoring pH electrode and on the recorded pH value: an in vitro study.
One of the problems concerning the continuous pH-monitoring technique is whether the relationship between pH measured by the electrode and pH in central and/or capillary fetal blood is constant. To test to what extent a fibrin clot deposited on the pH-electrode influenced the recorded value and the sensitivity of the electrode, the following in vitro study was performed. Fibrin was deposited on the pH-electrode by means of thromboplastin and fibrinogen, or by thromboplastin and whole blood. The deposition of a clot was verified by inspection of the electrode in a microscope. The time for stabilization of the recorded pH-value and the recorded pH-value was measured in standard calibration solutions before and after deposition of the fibrin clot and after decomposition of the fibrin clot by plasmin. FDP was measured in the decomposition solution. From the study it was obvious that the stabilization time of the electrode was considerably influenced by deposition of an "unphysiological" fibrin clot, less so if the clot was deposited by means of whole blood. The recorded pH-value was not influenced.
D004566:Electrodes; D005069:Evaluation Studies as Topic; D005337:Fibrin; D006863:Hydrogen-Ion Concentration; D013997:Time Factors
D005337:Fibrin