Immunoaffinity chromatography of human beta-hexosaminidase A.
A highly specific method for the purification of human beta-hexosaminidase A employing immunoaffinity chromatography is described. Using an antiserum against the unique antigenic determinant, alpha, of beta-hexosaminidase A, and elution with 8.0M urea, a 283-or 417-fold purification of the enzyme was obtained in a single step from crude human placental homogenate.
D000818:Animals; D002417:Cattle / Q000276:immunology; D002846:Chromatography, Affinity; D004588:Electrophoresis, Cellulose Acetate; D000939:Epitopes; D005260:Female; D005470:Fluorometry; D006596:Hexosaminidases / Q000302:isolation & purification*; D006801:Humans; D007106:Immune Sera; D005779:Immunodiffusion; D008722:Methods; D010920:Placenta / Q000201:enzymology*
D000939:Epitopes; D007106:Immune Sera; D006596:Hexosaminidases