A new preparative method for microspectrophotometric DNA-measurement in megakaryocytes, revealing a significant group of cells with a DNA content of 64 N.
Microspectrophotometric investigations of Feulgen-stained rat bone marrow megakaryocytes, comparing the traditional smear method and a new 1 G sedimentation and 40 degrees C evaporation (S. E.) method, have shown that the latter reveals a significant group of megakaryocytes (ca. 30 percent) with a DNA content of 64 N. This group is totally absent when the smear method is used.
D000818:Animals; D001854:Bone Marrow Cells; D004247:DNA / Q000032:analysis*; D005260:Female; D005269:Femur; D008533:Megakaryocytes / Q000032:analysis*; D051381:Rats; D013053:Spectrophotometry / Q000379:methods*; D013194:Staining and Labeling
D004247:DNA