Cytogenetic effect of bleomycin on human leukocytes in vitro.
Human leukocytes treated with bleomycin (BLM) for clinical use, at concentrations of 0.1, 0.5, 1.0, 10 and 50 mug/ml were studied. Both chromosome- and chromatid-type aberrations were observed. The groups of larger chromosomes were more affected at every concentration. At dosages from 0.1 to 10 mug/ml no significant difference of effects on chromosomes was observed. However, a dose-difference of about 500 times showed significant differences in effect both on the degree of chromosomal aberration and on mitotic indices.
D000328:Adult; D001761:Bleomycin / Q000494:pharmacology*; D002478:Cells, Cultured; D002842:Chromatids / Q000187:drug effects; D002869:Chromosome Aberrations; D004305:Dose-Response Relationship, Drug; D006801:Humans; D066298:In Vitro Techniques; D007962:Leukocytes / Q000187:drug effects* / Q000648:ultrastructure; D008297:Male; D008938:Mitosis / Q000187:drug effects
D001761:Bleomycin