Inhibition of 3H-thymidine incorporation into human lymphocytes by dialyzable material of streptokinase- streptodornase.
Using the 3H-thymidine incorporation test, the lymphocyte-stimulating activity of streptokinase-streptodornase (SK-SD, Varidase) and its fractions were evaluated. The dose-response curves of all the preparations except the third fraction had two peaks with the maximum around 5 and 40 mug. The dialyzable fraction of Sk-SD (D-SK-SD) occupying approximately half of the original sample had almost no lymphocyte-stimulating activity; and conversely, it had a potent inhibitory effect on the 3H-thymidine incorporation into lymphocytes that normally occurs with non-dialyzable SK-SD (ND-SK-SD), PPD and PHA-P. Therefore, it will be desirable to remove the dialyzable fraction from the commercial SK-SD preparation when it is used for the determination of the capacity of cellular immunity against tsk-sd.
D003956:Dialysis; D004305:Dose-Response Relationship, Drug; D000939:Epitopes; D006801:Humans; D037102:Lectins / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000378:metabolism*; D013299:Streptodornase and Streptokinase / Q000494:pharmacology*; D013936:Thymidine / Q000378:metabolism*; D014316:Tritium; D014373:Tuberculin
D000939:Epitopes; D037102:Lectins; D014373:Tuberculin; D014316:Tritium; D013299:Streptodornase and Streptokinase; D013936:Thymidine