[Biochemical and cytospectrophotometric analysis of the extraction of RNA from spinal cord cell structures].
By means of two-wavelength spectrophotometry, according to Tsanev and Markov, a stability of RNA content has been demonstrated in rabbit spinal cord sections treated with cold perchloric acid: it was only after 18 and particularly 48 hr incubation of the section in a 16% perchloric acid solution that the total tissue RNA began to be extracted. Cytospectrophotometrical study of the motoneurons of spinal cord anterior horns and perineuronal glial cells in gallocyanin -- chrome alum stained sections has shown a rapid loss of RNA under effect of the cold perchloric acid: as early as after a 2 hr treatment, about 2/3 of the whole cellular RNA was extracted from the motoneurons, while about 1/2 from their glial satellite cells. Hydrolysis of the rest of RNA was found out in the neurons and in the neuroglia only after a 18 hr extraction with the perchloric acid. Similarities and differences in the features of neuronal and glial RNA are discussed.
D000818:Animals; D003593:Cytoplasm / Q000032:analysis; D005260:Female; D008297:Male; D009046:Motor Neurons / Q000032:analysis; D009457:Neuroglia / Q000032:analysis; D010472:Perchlorates; D012313:RNA / Q000302:isolation & purification*; D011817:Rabbits; D013053:Spectrophotometry; D013116:Spinal Cord / Q000032:analysis / Q000166:cytology*; D013194:Staining and Labeling
D010472:Perchlorates; D012313:RNA
Biokhimicheskiĭ i tsitospektrofotometricheskiĭ analiz izvlecheniia RNK iz kletochnykh struktur spinnogo mozga