Oxygen binding of fetal and adult bovine hemoglobin in the presence of organic phosphates and uric acid riboside.
The P50 values of "stripped" fetal and adult bovine hemoglobin were 18.4 and 28.9 respectively. Neither the oxygen-hemoglobin dissociation curve nor the Hill coefficient, n, of fetal or adult bovine hemoglobin was affected by uric acid riboside (UAR), 2,3-diphosphoglyceric acid (2,3-DPG), adenosine triphosphate (ATP), or inositol pentaphosphate (IPP). Combinations of UAR and ATP with adult bovine hemoglobin or 2,3-DPG and ATP with fetal hemoglobin also had no effect. It was concluded that neither adult nor fetal bovine red cells contained an identifiable compound which affects the binding of oxygen to hemoglobin.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D004163:Diphosphoglyceric Acids / Q000494:pharmacology; D005260:Female; D005319:Fetal Hemoglobin / Q000378:metabolism*; D006441:Hemoglobin A / Q000378:metabolism; D006454:Hemoglobins / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007295:Inositol Phosphates / Q000494:pharmacology; D009943:Organophosphorus Compounds / Q000494:pharmacology*; D010101:Oxygen Consumption; D011247:Pregnancy; D012263:Ribonucleosides / Q000494:pharmacology*; D014527:Uric Acid / Q000031:analogs & derivatives* / Q000494:pharmacology
D004163:Diphosphoglyceric Acids; D006454:Hemoglobins; D007295:Inositol Phosphates; D009943:Organophosphorus Compounds; D012263:Ribonucleosides; C012090:uric acid riboside; D014527:Uric Acid; D000255:Adenosine Triphosphate; D006441:Hemoglobin A; D005319:Fetal Hemoglobin