Purification of alpha 2-macroglobulin with trypsin-like activity from pleural fluids.
An alpha 2-macroglobulin with trypsin-like activity has been purified from pleural fluids of patients suffering from chronic pancreatitis. The isolation procedure includes ammonium sulphate precipitation, gel-filtration on Sephadex G-200 and DEAE-cellulose chromatography. It gives 46-fold purification of alpha 2-macroglobulin with a 13% recovery. Based on titration experiments with pancreatic inhibitor, the protein from three different patients contained 0.28, 0.46 and 0.80 mol of trypsin-like protease per mol of alpha 2-macroglobulin.
D002850:Chromatography, Gel; D002908:Chronic Disease; D006801:Humans; D009097:Multienzyme Complexes / Q000302:isolation & purification*; D010195:Pancreatitis / Q000201:enzymology; D010996:Pleural Effusion / Q000201:enzymology*; D014357:Trypsin / Q000378:metabolism*; D000511:alpha-Macroglobulins / Q000302:isolation & purification* / Q000378:metabolism
D009097:Multienzyme Complexes; D000511:alpha-Macroglobulins; D014357:Trypsin