title: The staining of Brunner's gland and other neutral mucins by carmine, hematoxylin and orcein in alkaline solutions.
abstract: Brunner's glands and other neutral mucins may be stained red, brownish red, and violet, respectively, by carmine, hematoxylin, and orcein from appropriate alkaline solutions. Carmine and hematoxylin in concentrations of 0.2-1% are dissolved in 60-70% alcohol containing 1% potassium carbonate; orcein is used in a 0.2% alcoholic solution of sodium hydroxide. Staining times are 15 to 30 minutes. The stained sections are rinsed in 95% or absolute alcohol prior to xylene and mounting. The staining of these mucins is blocked by mild bromine oxidation. By using alcian blue 0.1% in 3% acetic acid for 5 minutes prior to the above stains, mucins may be characterized in the same preparation as acid, neutral or mixed.
mesh_terms: D000468:Alkalies; D002011:Brunner Glands / Q000032:analysis*; D002329:Carmine; D004386:Duodenum / Q000032:analysis*; D006416:Hematoxylin; D006651:Histocytochemistry / Q000379:methods*; D006801:Humans; D009077:Mucins / Q000032:analysis*; D012996:Solutions; D013194:Staining and Labeling
chemical_list: D000468:Alkalies; D009077:Mucins; D012996:Solutions; D002329:Carmine; D006416:Hematoxylin