In vitro cultivation of prostatic epithelium.
A tissue culture procedure for in vitro cultivation of human prostatic tissue that utilizes the enzyme collagenase to disrupt the prostate specimen is described. Collagenase effectively disperses the tissue stroma into single cells which are easily separated from the largely intact glandular structures. The isolated glandular fragments then are used to initiate monolayers of prostatic epithelium in vitro.
D002469:Cell Separation; D002478:Cells, Cultured; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods*; D004848:Epithelium / Q000254:growth & development; D006801:Humans; D008297:Male; D003012:Microbial Collagenase; D011467:Prostate / Q000254:growth & development*; D011470:Prostatic Hyperplasia / Q000473:pathology
D003470:Culture Media; D003012:Microbial Collagenase