Multiple sclerosis and parainfluenza 1 virus. History of the isolation of the virus and expression of phenotypic differences between the isolated virus and Sendai virus.
54 cultures were established from brain tissue obtained 2-3 hrs after death from 1 case of multiple sclerosis and 30 cultures from another case. Following fusion with indicator cells in the presence of lysolecithin, a parainfluenza type 1 virus (6/94 virus) was isolated from cultures representing one plaque area in the first case and one plaque area in the second case. A cell line chronically infected with the 6/94 virus has been maintained for more than 100 passages in vitro. A close relationship to the Sendai Hemagglutinating Virus of Japan (HVJ) is indicated from RNA-RNA hybridization and the patterns of electrophoretic mobilities of viral polypeptides. Conversely, differences in optima for growth-requirement temperatures, hemolytic activity and the capability to fuse mammalian cells, distinguishes 6/94 virus and HVJ as distinct phenotypic entities of a closely related genotype.
D000328:Adult; D002459:Cell Fusion; D002478:Cells, Cultured; D003429:Cross Reactions; D005260:Female; D006383:Hemadsorption Inhibition Tests; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D008244:Lysophosphatidylcholines; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D010222:Parainfluenza Virus 1, Human / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D010455:Peptides / Q000032:analysis; D014764:Viral Proteins / Q000032:analysis; D014776:Virus Cultivation
D008244:Lysophosphatidylcholines; D010455:Peptides; D014764:Viral Proteins