Establishment of a cell line and its clonal sublines from a patient with hepatoblastoma.
By cloning from cultivation in vitro of a human hepatoblastoma 3 epithelial cell lines were established, all of which produced alpha-fetoprotein and one of which did albumin in culture. Observation with electron microscopy demonstrated desmosomes and glycogen granules in the cytoplasm of these cells. The morphology, growth characteristics, alpha-fetoprotein production, heterotransplantability, and karyology of the cloned cells suggested that these clonal lines were derived from the tumor cells and retained their malignant characteristics.
D000418:Albumins / Q000032:analysis; D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000032:analysis / Q000473:pathology*; D002460:Cell Line; D002999:Clone Cells / Q000648:ultrastructure; D006224:Cricetinae; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D006801:Humans; D007223:Infant; D007621:Karyotyping; D008113:Liver Neoplasms / Q000032:analysis / Q000473:pathology*; D008297:Male; D009368:Neoplasm Transplantation; D014183:Transplantation, Heterologous; D000509:alpha-Fetoproteins / Q000032:analysis
D000418:Albumins; D000509:alpha-Fetoproteins