title: Immobilization of neuraminidase and its potential application in the modification of cell surfaces.
abstract: Vibrio cholerae neuraminidase was immobilized on the inside of 1.0 mm inner diameter nylon tubing with retention of enzyme activity, when assayed at 37 degrees C and pH 5.5 with mucin as substrate. The stabilities of the immobilized and soluble enzymes were similar for up to 3 hr at 37 degrees C. Preliminary data indicated that immobilized neuraminidase will release sialic acid from the surface of leukemic AKR mouse thymus and spleen lymphocytes; however, the level of immobilized enzyme activity needs to be increased for practical applications. With this improvement immobilized neuraminidase could become a novel preparation for carrying out cell surface modifications with minimal enzyme contamination of the cell.
mesh_terms: D000818:Animals; D004355:Drug Stability; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005260:Female; D006863:Hydrogen-Ion Concentration; D008214:Lymphocytes / Q000378:metabolism; D051379:Mice; D009077:Mucins / Q000378:metabolism; D009439:Neuraminidase / Q000378:metabolism*; D012794:Sialic Acids / Q000302:isolation & purification; D013696:Temperature; D014734:Vibrio cholerae / Q000201:enzymology*
chemical_list: D004800:Enzymes, Immobilized; D009077:Mucins; D012794:Sialic Acids; D009439:Neuraminidase