Direct method for estimating the total gastrin cell number in the stomach of rats.
An original and simple method for counting the total G cell number in the stomach of small rodents is described. Staining the G cells with the peroxidase-antibody technique and counterstaining the nucleus make it possible to correct the nuclear overestimation. Counting directly the total number of G cells in sections from the entire stomach avoids errors due to tissue retraction and to unprecise delineation of the antro-fundic limit.
D000818:Animals; D002452:Cell Count / Q000379:methods*; D002467:Cell Nucleus / Q000648:ultrastructure; D005753:Gastric Mucosa / Q000166:cytology* / Q000378:metabolism; D005755:Gastrins / Q000378:metabolism; D006652:Histological Techniques; D008297:Male; D051381:Rats; D013194:Staining and Labeling
D005755:Gastrins