Typing for HLA-D determinants. comparison of typing results using homozygous stimulating cells and primed cultures.
Typing for HLA-D determinants, using primed lymphocyte typing (PLT), has been compared to the results obtained using conventional homozygous stimulating cell primary MLC tests. The HLA-Dwl, -Dw2 and -Dw3 specificities were studied. Preliminary results indicate that these two methods essentially type for the HLA-D determinants and that reproducible results can be obtained employing the PLT technique after 24 hours of incubation in the secondary cultures.
D000939:Epitopes; D006649:Histocompatibility Antigens; D006650:Histocompatibility Testing; D006720:Homozygote; D006801:Humans; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology*; D008937:Mitomycins / Q000494:pharmacology
D000939:Epitopes; D006649:Histocompatibility Antigens; D008937:Mitomycins