title: Ability of tryptophan tRNA to hybridize with 35S RNA of avian myeloblastosis virus and to prime reverse transcription in vitro.
abstract: Selected species of 4S RNA of chick embryo cells will hybridize in vitro with 35S RNA of avian myeloblastosis virus. A major tRNA component of the hybridizable 4S RNA is tryptophan tRNA. A hybrid prepared from purified tryptophan tRAN and 35S RNA of avian myeloblastosis virus in vitro is an efficient templateprimer for DNA synthesis catalyzed by reverse transcriptase (RNA-dependent DNA polymerase).
mesh_terms: D000818:Animals; D001354:Avian Leukosis Virus; D009189:Avian Myeloblastosis Virus / Q000201:enzymology; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D002642:Chick Embryo; D002852:Chromatography, Ion Exchange; D004789:Enzyme Activation; D007700:Kinetics; D009693:Nucleic Acid Hybridization; D012343:RNA, Transfer / Q000494:pharmacology; D012367:RNA, Viral / Q000494:pharmacology; D012194:RNA-Directed DNA Polymerase; D014364:Tryptophan
chemical_list: D012367:RNA, Viral; D014364:Tryptophan; D012343:RNA, Transfer; D012194:RNA-Directed DNA Polymerase