Prostaglandin E-2-9-ketoreductase in ovarian tissues.
The existence of the enzyme prostaglandin E-2-9-ketoreductase which can convert prostaglandin E-2 to prostaglandin F-2 alpha was indicated in experiments with pig and human ovarian tissues in vitro, using radioimmunoassay methods and a superfusion technique. Further studies involving radiotracer techniques demonstrated that the enzyme was localized in the high-speed (105 000 g) supernatant fraction of human, pig and rat luteal tissue and human stromal tissue. The enzyme was shown to be NADPH-dependent and its activity in luteal tissue increased in the order : pig less than human less than rat.
D000818:Animals; D005260:Female; D006801:Humans; D006911:Hydroxyprostaglandin Dehydrogenases / Q000032:analysis*; D066298:In Vitro Techniques; D009249:NADP / Q000378:metabolism; D010053:Ovary / Q000201:enzymology* / Q000378:metabolism; D011458:Prostaglandins E / Q000378:metabolism; D011460:Prostaglandins F / Q000378:metabolism; D051381:Rats; D013552:Swine
D011458:Prostaglandins E; D011460:Prostaglandins F; D009249:NADP; D006911:Hydroxyprostaglandin Dehydrogenases