Identification of gamma-like DNA polymerase from sea urchin embryos.
A gamma-like DNA polymerase devoid of DNA polymerase-alpha and -beta activities was prepared from the nuclear fraction of blastulae of the sea urchin, Hemicentrotus pulcherrimus. The enzyme sedimented at the position of an approximate sedimentation coefficient of 3.3 S under high salt conditions by sucrose gradient centrifugation. An isoelectric point was determined to be pH 5.8. The enzyme activity was sensitive to sulfhydryl blocking reagents. Poly(rA) . oligo(dT)12--18 followed by poly(dA) . oligo(dT)12--18 was effectively utilized as a template-primer. From the above results, this polymerase seems to resemble the vertebrate DNA polymerase-gamma.
D000818:Animals; D002412:Cations / Q000494:pharmacology; D002467:Cell Nucleus / Q000201:enzymology; D002499:Centrifugation, Density Gradient; D004259:DNA-Directed DNA Polymerase / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D007700:Kinetics; D010710:Phosphates / Q000494:pharmacology; D012617:Sea Urchins / Q000196:embryology / Q000201:enzymology*; D013379:Substrate Specificity; D013439:Sulfhydryl Reagents / Q000494:pharmacology
D002412:Cations; D010710:Phosphates; D013439:Sulfhydryl Reagents; D004259:DNA-Directed DNA Polymerase