Immunogenic and immunosuppressive components in soluble preparations from Moloney murine leukemia virus-induced tumor cells.
Soluble extracts from Moloney virus-induced leukemia cells were shown to contain immunogenic and immunosuppressive components. Immunogenic components were divided into two classes based on their ability to :(1) combine with humoral antibody to inhibit cytotoxic activity; or (2) stimulate blastogenesis in normal syngeneic lymphocytes. Immunosuppressive components were demonstrated by the inhibition of DNA synthesis in normal syngeneic lymphocytes and suppression of a mixed lymphocyte reaction. Stimulating components in many soluble preparations were completely masked by immunosuppressive components. However, all three components could be resolved by Sephadex G200 chromatography.
D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis; D002850:Chromatography, Gel; D004273:DNA, Neoplasm / Q000096:biosynthesis; D000939:Epitopes; D007111:Immunity, Cellular; D007114:Immunization; D007165:Immunosuppression Therapy; D007942:Leukemia, Experimental / Q000276:immunology*; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D017710:Lymphocyte Transfusion; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008937:Mitomycins / Q000494:pharmacology; D008979:Moloney murine leukemia virus / Q000276:immunology*; D009368:Neoplasm Transplantation; D012995:Solubility; D013154:Spleen / Q000276:immunology
D000951:Antigens, Neoplasm; D004273:DNA, Neoplasm; D000939:Epitopes; D008937:Mitomycins