Effects of cell culture and laboratory conditions on type 2 dengue virus infectivity.
The stability of type 2 dengue virus to exposure to a variety of laboratory conditions was determined. Suckling mouse brain passage virus was adapted for growth in BHK-21 cells, and plaque assays were performed using a tragacanth gum overlay. A three- to fourfold increase in plaque size could be obtained if monolayers were subconfluent at time of inoculation. Incubation of virus for 24 h at 37 degrees C, pH 6.5, or in buffer containing 1 mM ethylenediaminetetraacetate considerably reduced virus infectivity as compared with virus incubated for the same period at 4 degrees C, pH 8.0, or in buffer with or without 1 mM CaCl2 and 1 mM MgCl2. Multiple freezing and thawing of virus tissue culture medium containing 10% fetal calf serum did not reduce virus infectivity.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002460:Cell Line; D002478:Cells, Cultured; D006224:Cricetinae; D003716:Dengue Virus / Q000254:growth & development* / Q000472:pathogenicity*; D005615:Freezing; D006863:Hydrogen-Ion Concentration; D007668:Kidney; D008274:Magnesium / Q000494:pharmacology; D013696:Temperature; D010948:Viral Plaque Assay; D014779:Virus Replication / Q000187:drug effects
D008274:Magnesium; D002118:Calcium