Ability of tryptophan tRNA to hybridize with 35S RNA of avian myeloblastosis virus and to prime reverse transcription in vitro.
Selected species of 4S RNA of chick embryo cells will hybridize in vitro with 35S RNA of avian myeloblastosis virus. A major tRNA component of the hybridizable 4S RNA is tryptophan tRNA. A hybrid prepared from purified tryptophan tRAN and 35S RNA of avian myeloblastosis virus in vitro is an efficient templateprimer for DNA synthesis catalyzed by reverse transcriptase (RNA-dependent DNA polymerase).
D000818:Animals; D001354:Avian Leukosis Virus; D009189:Avian Myeloblastosis Virus / Q000201:enzymology; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D002642:Chick Embryo; D002852:Chromatography, Ion Exchange; D004789:Enzyme Activation; D007700:Kinetics; D009693:Nucleic Acid Hybridization; D012343:RNA, Transfer / Q000494:pharmacology; D012367:RNA, Viral / Q000494:pharmacology; D012194:RNA-Directed DNA Polymerase; D014364:Tryptophan
D012367:RNA, Viral; D014364:Tryptophan; D012343:RNA, Transfer; D012194:RNA-Directed DNA Polymerase