The specificity of antisera raised by oestradiol-17beta-3-hemisuccinyl-bovine serum albumin.
The preparation of pure oestradiol-17beta-3-hemisuccinyl-bovine serum albumin conjugate is described. Contrary to previous findings this antigen raised reasonably specific antisera in rabbits which possessed a cross reaction of only 2.0% with oestrone, and 0.8% with oestriol. The production of this specific antisera is considered to be due to the high purity of the antigen. The role of the C-3 phenolic hemisuccinyl linkage of the antigen in raising this specific antisera is discussed.
D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D055598:Chemical Phenomena; D002621:Chemistry; D003429:Cross Reactions; D000939:Epitopes; D004958:Estradiol / Q000031:analogs & derivatives* / Q000276:immunology; D007106:Immune Sera; D012710:Serum Albumin, Bovine
D000939:Epitopes; D007106:Immune Sera; D012710:Serum Albumin, Bovine; D004958:Estradiol