title: Electroncytochemical and biochemical demonstration of guanylate cyclase activity in the pancreatic islet.
abstract: With a cytochemical method using guanylyl imidodiphosphate as a substrate, the guanylate cyclase activity was localized on the plasma membrane of A, B and D cells of islets of Langerhans isolated from the rat. Adequate control experiments were performed by a double-blind method. Parallel biochemical assay showed that guanylate cyclase activity was not completely lost after fixation with 1% glutaraldehyde and incubation with 4 mM lead nitrate. Furthermore, the depressed activity was still stimulatable with acetylcholine.
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D006162:Guanylate Cyclase / Q000032:analysis* / Q000378:metabolism; D006165:Guanylyl Imidodiphosphate / Q000378:metabolism; D006651:Histocytochemistry; D007515:Islets of Langerhans / Q000201:enzymology*; D008297:Male; D051381:Rats
chemical_list: D006165:Guanylyl Imidodiphosphate; D006162:Guanylate Cyclase; D000109:Acetylcholine