Isolation of drugs from autopsy material by XAD-2 adsorption-elution technique. A routine procedure.
The method of adsorption of drugs on Amberlite XAD-2 resin, followed by differential elution was under study during 1 1/2 year of routine use. The method allows a separation of acidic and basic drugs and--due to acid hydrolysis step before adsorption--assures better recovery of conjugated and protein-bound drugs. The amounts of various drugs, found in autopsy cases by the XAD-2 method were usually higher than those found by solvent extraction. The method applied requires 20 g of sample (biofluid or tissue) for general toxicological analysis.
D000327:Adsorption; D000928:Antidepressive Agents / Q000032:analysis; D001344:Autopsy; D002852:Chromatography, Ion Exchange; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D004364:Pharmaceutical Preparations / Q000032:analysis*; D010640:Phenothiazines / Q000032:analysis; D011044:Poland; D014116:Toxicology / Q000379:methods*
D000928:Antidepressive Agents; D004364:Pharmaceutical Preparations; D010640:Phenothiazines