Observations on specific giemsa staining of the Y and on selective oil destaining of the chromosomes.
The human Y chromosome can be differentially stained with Giemsa using simple procedures. This phenomenon is strikingly to that observed with quinacrine fluorescence. The specific Giemsa-Y stain may be selectively removed by the action of an oil. The same oil, under certain conditions, selectively removes Giemsa stain from all chromosomes, resulting in R- and T-banding patterns. These bands, which are obtained through subtraction of dye from Giemsa-stained chromosomes, allow slides to be further processed.
D006570:Heterochromatin / Q000032:analysis; D006801:Humans; D008297:Male; D009821:Oils; D011796:Quinacrine; D012730:Sex Chromosomes / Q000032:analysis*; D012995:Solubility; D013194:Staining and Labeling
D006570:Heterochromatin; D009821:Oils; D011796:Quinacrine