Epidermal surface saccharides reactive with phytohemagglutinins and pemphigus antigen.
Inorder to study the relationship between epidermal surface saccharides and pemphigus antigen(s), fluorescein-labelled Concanavalin A (Con A) and Phytohemagglutinin-P (PHA-P) were used. These phytohemagglutinins were found to bind with the intercellular areas of human epidermis. Alpha-methyl-D-mannoside and alpha-methyl-D-glucoside inhibited the epidermal intercellular staining pattern produced by ConA-FITC, while N-acetyl-D-galactosamine blocked the same staining pattern produced by PHA-P-FITC. Normal human skin reacted with pemphigus antibody and pemphigus skin with the deposition of IgG both gave a positive intercellular staining pattern with fluorescein labelled phytohemagglutinins. Our data indicated the non-identity of the binding of Con A and PHA-P, and pemphigus antigen(s).
D000368:Aged; D000906:Antibodies; D000941:Antigens; D001666:Binding Sites, Antibody; D001706:Biopsy; D003208:Concanavalin A / Q000494:pharmacology*; D005260:Female; D005456:Fluorescent Dyes; D005688:Galactosamine / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D006801:Humans; D007074:Immunoglobulin G; D037102:Lectins / Q000494:pharmacology*; D008358:Mannose / Q000494:pharmacology; D009005:Monosaccharides / Q000276:immunology*; D010392:Pemphigus / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D012867:Skin / Q000276:immunology* / Q000473:pathology; D013194:Staining and Labeling
D000906:Antibodies; D000941:Antigens; D005456:Fluorescent Dyes; D007074:Immunoglobulin G; D037102:Lectins; D009005:Monosaccharides; D003208:Concanavalin A; D005688:Galactosamine; D005947:Glucose; D008358:Mannose