Acid mucopolysaccharides in hair papillae of the stump-tailed macaque (Macaca speciosa).
Acid mucopolysaccharides in dermal papillae of hair follicles from both bald and on-bald regions of the scalp of stump-tailed macaques were studies histochemically. Alcian Blue, Azure A and Periodic acid Schiff methods were used for staining mucopolysaccharides, and Bromphenol Blue for staining basic proteins. In an attempt to identify various polyanions, staining was carried out with Alcian Blue containing different concentrations of electrolytes. Methylation, saponification, mild acid hydrolysis and digestion with streptomyces or testicular hyaluronidase, chondroitinase ABC, or sialidase, were also used. The results indicate that chondroitin sulphate B is present in the papillae of terminal hair follicles in early and intermediate anagen, and degraded chondroitin sulphates are present in the papillae of vellus and terminal hair follicles in late anagen.
D000818:Animals; D006025:Glycosaminoglycans / Q000032:analysis*; D006197:Hair / Q000032:analysis* / Q000166:cytology; D000882:Haplorhini; D006651:Histocytochemistry; D006821:Hyaluronoglucosaminidase; D008251:Macaca; D008722:Methods; D012867:Skin / Q000032:analysis* / Q000166:cytology; D013194:Staining and Labeling
D006025:Glycosaminoglycans; D006821:Hyaluronoglucosaminidase