Superoxide dismutase from Mycobacterium species, strain Takeo.
Superoxide dismutase from Mycobacterium species, strain Takeo, has been purified to homogeneity as judged by disc gel electrophoresis and ultracentrifugation. The enzyme was found to have a molecular weight of approximately 61 500 by sedimentation equilibrium and to contain manganese by atomic absorption and electron spin resonance spectra. The amino acid composition was also determined. The enzyme was considerably stable to the treatment with sodium dodecyl sulfate; unless incubating at 80 degrees C for 2 min, it was not completely dissociated into the subunits. The molecular weight of the subunit was found to be approximately 21 000. Antibodies against the superoxide dismutase were produced by immunization of rabbits with the enzyme, and the gamma-globulin fraction was purified. Superoxide dismutase preparations obtained from various species of mycobacteria and nocardia cross-reacted to different degrees with these antibodies on the Ouchterlony double diffusion plates. Comparative immunological studies indicated that strain Takeo might be most closely related to Myobacterium smegmatis among species of mycobacteria and nocardia tested. The antibodies against superoxide dismutase may be used as a valuable tool for the classification of mycobacteria.
D000596:Amino Acids / Q000032:analysis; D003429:Cross Reactions; D004355:Drug Stability; D000939:Epitopes; D008345:Manganese / Q000032:analysis; D008970:Molecular Weight; D009161:Mycobacterium / Q000145:classification / Q000201:enzymology*; D012967:Sodium Dodecyl Sulfate; D013057:Spectrum Analysis; D013482:Superoxide Dismutase / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification
D000596:Amino Acids; D000939:Epitopes; D012967:Sodium Dodecyl Sulfate; D008345:Manganese; D013482:Superoxide Dismutase