[Neoantigens on cells transformed by SV40. IV. - A quantitative study of antigenic sites in a cell line (TSV5C12) (author's transl)].
The antigenic sites at the membrane of SV40-transformed hamster fibroblasts (TSV5C12) were studied using isotope techniques. A specific fixation of antibodies directed against SV40 coded antigen had previously been shown using a cytotoxicity test in presence of fresh guinea pig complement. Using the "paired-label" technique, described by Boone et al., approximatively 7.2. 10(7) fixing site for the specific antibodies against SV40 coded antigen(s), were found. The fixation of antibodies onto the cells varied with the time of culture of cells. It was not possible to determine whether the number of antigenic sites, or their accessibility to the antibodies, or their susceptibility to complement, was dependent on the cell cycle.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000302:isolation & purification; D000937:Antigen-Antibody Reactions; D000951:Antigens, Neoplasm / Q000032:analysis*; D001666:Binding Sites, Antibody; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D006224:Cricetinae; D003601:Cytotoxicity Tests, Immunologic; D000939:Epitopes; D005347:Fibroblasts / Q000276:immunology; D011817:Rabbits; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D013539:Simian virus 40 / Q000276:immunology*; D014357:Trypsin / Q000378:metabolism
D000888:Antibodies, Anti-Idiotypic; D000951:Antigens, Neoplasm; D000939:Epitopes; D011947:Receptors, Antigen, B-Cell; D014357:Trypsin
Néo-antigenes des cellules transformées par le virus SV40. IV. - Etude quantitative des sites antigéniques d'une lignée cellulaire (TSV5C12)