On the nature of the external surface of cultured human embryo fibroblasts: an ultrastructural and cytochemical analysis utilising stain and lectin probes.
Surfaces of human embryo fibroblasts in vitro were reacted with stain and lectin probes for carbohydrate moeities either after or in the absence of treatment with low concentrations of surface-active enzymes and EDTA. Only testicular hyaluronidase significantly suppressed affinity-binding of all three agents, suggesting that acidic glycosaminoglycans are principle components of the cell's exterior.
D000818:Animals; D001665:Binding Sites; D002241:Carbohydrates / Q000302:isolation & purification; D002417:Cattle; D002462:Cell Membrane / Q000032:analysis* / Q000201:enzymology / Q000648:ultrastructure; D003208:Concanavalin A / Q000302:isolation & purification; D004622:Embryo, Mammalian / Q000166:cytology; D004798:Enzymes / Q000378:metabolism; D005347:Fibroblasts / Q000032:analysis* / Q000648:ultrastructure; D006025:Glycosaminoglycans / Q000302:isolation & purification; D006801:Humans; D037102:Lectins / Q000032:analysis; D013194:Staining and Labeling; D013499:Surface Properties; D013501:Surface-Active Agents
D002241:Carbohydrates; D004798:Enzymes; D006025:Glycosaminoglycans; D037102:Lectins; D013501:Surface-Active Agents; D003208:Concanavalin A