Comparsion of the properties of two antigen-induced guinea pig lymphokines.
The properties of macrophage migration inhibition factor (MIF) and mitogenic factor (MF) were compared using culture supernatants of antigen-stimulated lymph node cells from inbred guinea pigs. Gel filtration on Sephadex G-100 indicated molecular weights of about 60,000 and 25,000 for MIF and MF, respectively. The lymphokines also differed with respect to heat sensitivity, MIF being largely inactivated by 60 degrees C for 20 min, whereas MF was unaffected by this treatment. The time course of production, antigen specificity of induction, and susceptibility to neuraminidase were also examined.
D000818:Animals; D002417:Cattle / Q000276:immunology; D002464:Cell Migration Inhibition; D002478:Cells, Cultured; D002850:Chromatography, Gel; D003016:Clostridium perfringens / Q000201:enzymology; D004140:Dinitrophenols / Q000276:immunology; D006168:Guinea Pigs; D006801:Humans; D007114:Immunization; D008198:Lymph Nodes / Q000166:cytology; D008214:Lymphocytes / Q000276:immunology; D008222:Lymphokines / Q000302:isolation & purification*; D008263:Macrophage Migration-Inhibitory Factors / Q000302:isolation & purification*; D008938:Mitosis; D009439:Neuraminidase; D012709:Serum Albumin; D013696:Temperature; D005719:gamma-Globulins
D004140:Dinitrophenols; D008222:Lymphokines; D008263:Macrophage Migration-Inhibitory Factors; D012709:Serum Albumin; D005719:gamma-Globulins; D009439:Neuraminidase