Specific adsorption of IgM antibody onto H-2-activated mouse T lymphocytes.
Evidence is presented to support the contention that IgM demonstrable by surface immunofluorescent staining on H-2-activated T cells represents specifically adsorbed B-cell-derived alloantibody. T cells activated to H-2 determinants expressed surface IgM only when the progenitor cell populations contained B lymphocytes. IgM was not detected on T cells activated to determinants which fail to stimulate alloantibody production (e.g., M-locus determinants). In addition, IgM-negative H-2 activated T cells (derived from B-cell-depleted lymphoid cells), unlike M-locus-activated T cells, adsorbed IgM in a specific manner when incubated in vitro with "early bleed" antisera raised against the activating H-2 determinants.
D000327:Adsorption; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D001666:Binding Sites, Antibody; D000939:Epitopes; D006680:HLA Antigens; D006649:Histocompatibility Antigens; D007075:Immunoglobulin M / Q000378:metabolism*; D007518:Isoantibodies / Q000378:metabolism; D008196:Lymph / Q000276:immunology; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology* / Q000637:transplantation; D014184:Transplantation, Homologous
D000939:Epitopes; D006680:HLA Antigens; D006649:Histocompatibility Antigens; D007075:Immunoglobulin M; D007518:Isoantibodies