Sulfhydryl-dependent thermal aggregation of human gamma globulin: augmentation by hyaluronic acid.
Hyaluronic acid (4 mg/ml) augmented elevenfold the copper-catalyzed (7 muM) thermal (63 degrees C, 2 hours) aggregation of human gamma globulin (2 mg/ml) in 0.075 M phosphate buffer, pH 7.4. Almost no augmentation of aggregation occurred with hyaluronidase-treated hyaluronate. Hyaluronate-augmented copper-catalyzed thermal aggregation was inhibited by L-histidine, gold thiomalate, N-ethylmaleimide, p-chloromercuribenzoic acid, and ethylenediaminetetraacetic acid. Together with previous reports of a decreased blood histidine concentration in rheumatoid arthritis, these studies provide a possible explanation for the affinity of this disease for joints.
D002384:Catalysis; D011232:Chemical Precipitation; D002729:Chloromercuribenzoates / Q000494:pharmacology; D003300:Copper / Q000494:pharmacology; D003864:Depression, Chemical; D004492:Edetic Acid / Q000494:pharmacology; D005033:Ethylmaleimide / Q000494:pharmacology; D006052:Gold Sodium Thiomalate / Q000494:pharmacology; D006358:Hot Temperature; D006801:Humans; D006820:Hyaluronic Acid / Q000494:pharmacology*; D066298:In Vitro Techniques; D013438:Sulfhydryl Compounds / Q000494:pharmacology*; D005719:gamma-Globulins
D002729:Chloromercuribenzoates; D013438:Sulfhydryl Compounds; D005719:gamma-Globulins; D006052:Gold Sodium Thiomalate; D003300:Copper; D006820:Hyaluronic Acid; D004492:Edetic Acid; D005033:Ethylmaleimide