Alcohol dehydrogenase (ADH) in yeasts. II. NAD+-and NADP+-dependent alcohol dehydrogenases in Saccharomycopsis lipolytica.
In Sm. lipolytica one NAD+-dependent and three NADP+-dependent alcohol dehydrogenases are detectable by polyacrylamide gelelectrophoresis. The NAD+-dependent ADH (ADH I), with a molecular weight of 240,000 daltons, reacts more intensively with long-chain alcohols (octanol) than with short-chain alcohols (methanol, ethanol). The ADH I is not or only minimally subject to glucose repression. Besides the ADH I band no additional inducible NAD+-dependent ADH band is gel-electrophoretically detectable during growth of yeast cells in medium containing ethanol or paraffin. The ADH I band is very probably formed by two ADH enzymes with the same electrophoretic mobility. The NADP+-dependent alcohol dehydrogenases (ADH II--IV) react with methanol, ethanol and octanol with different intensity. In polyacrylamide gradients two bands of NADP+-dependent ADH are detectable: one with a molecular weight of 70,000 daltons and the other with 120,000 daltons. The occurrence of the three NADP+-dependent alcohol dehydrogenases is regulated by the carbon source of the medium. Sm. lipolytica shows a high tolerance against allylalcohol. Resistant mutants can be isolated only at concentrations of 1 M allylalcohol in the medium. All isolates of allylalcohol-resistant mutants show identical growth in medium containing ethanol as the wild type strain.
D000085:Acetates / Q000378:metabolism; D000429:Alcohol Oxidoreductases / Q000032:analysis / Q000378:metabolism*; D001203:Ascomycota / Q000201:enzymology*; D004794:Enzyme Repression; D000431:Ethanol / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D007527:Isoenzymes / Q000032:analysis / Q000378:metabolism*; D008970:Molecular Weight; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D012442:Saccharomycopsis / Q000201:enzymology* / Q000378:metabolism
D000085:Acetates; D007527:Isoenzymes; D009243:NAD; D000431:Ethanol; D009249:NADP; D000429:Alcohol Oxidoreductases; D005947:Glucose