[Interaction of aliphatid alcohols with cytochrome P-450 from rat liver microsomes].
The interaction of alyphatic alcohols and cyclohexanol with cytochrome P-450 in microsomes has been investigated. All alchohols induced the modified 11 type spectral changes by mixing with microsomes. These changes are characterized by lambdamax = 412 and lambdamin = 380-382 nm in difference spectra. The dissociation constants of the alcohol cytochrome P-450 complexes are determined. On this dissociation constants influence pH and Triton X-100 presence. The interaction of the alcohols with cytochrome P-450 in phosphate buffer pH = 6,0 in the detergents absence is characterized by one dissociation constant for MeOH, EtOH, n-BuOH and cyclohexanol and by two dissociation constants for i-PrOH, i-BuOH and tert.-BuOH. The interaction of the alcohols with cytochrome P-450 in Tris-HCL-buffer (pH 7.5) in the Triton X-100 presence is characterized for all above alcohols by the dissociations constants, which are described by Taft equation with coefficient rho =-1.55. This fact confirms the interaction of alcohols HO-groups with heme iron of cytochrome P-450. The scheme of interaction of alcohols with cytochrome P-450 is discussed.
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D003511:Cyclohexanols; D003577:Cytochrome P-450 Enzyme System; D005233:Fatty Alcohols; D006863:Hydrogen-Ion Concentration; D008862:Microsomes, Liver / Q000378:metabolism*; D011092:Polyethylene Glycols; D051381:Rats
D003511:Cyclohexanols; D005233:Fatty Alcohols; D011092:Polyethylene Glycols; D003577:Cytochrome P-450 Enzyme System
Vzaimodeĭstvie alifaticheskikh spirtov s tsitokhromom P-450 mikrosom pecheni krysy.