Transmission of stability (telestability) in deoxyribonucleic acid. Physical and enzymatic studies on the duplex block polymer d(C15A15) - d(T15G15).
The properties of the duplex block polymer d(C15A15) - d(T15G15) were examined by thermal denaturation and nuclease susceptibility studies in the absence and presence of drugs (actinomycin and netropsin) which bind specifically to only one end of the block polymer. The nucleotide composition of one region of this synthetic double-helical DNA affected the properties of a contiguous but remote region. Furthermore, the binding of actinomycin influenced the properties of both the binding and nonbinding regions. These findings suggest a mechanism for gene regulation at a distance.
D000249:Adenosine Monophosphate / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002250:Carbon Radioisotopes; D055598:Chemical Phenomena; D002621:Chemistry; D004247:DNA / Q000138:chemical synthesis; D003609:Dactinomycin / Q000494:pharmacology; D004926:Escherichia coli / Q000201:enzymology; D005092:Exonucleases / Q000378:metabolism; D006146:Guanidines / Q000494:pharmacology; D006358:Hot Temperature; D009691:Nucleic Acid Denaturation; D009842:Oligopeptides / Q000494:pharmacology; D011108:Polymers; D011758:Pyrroles / Q000494:pharmacology; D014316:Tritium
D000900:Anti-Bacterial Agents; D002250:Carbon Radioisotopes; D006146:Guanidines; D009842:Oligopeptides; D011108:Polymers; D011758:Pyrroles; D014316:Tritium; D003609:Dactinomycin; D000249:Adenosine Monophosphate; D004247:DNA; D005092:Exonucleases