Serodiagnostic application of immunohistoperoxidase reactions on antigen-coupled agarose beads.
Agarose beads to which antigens were covalently bound were subjected to indirect immunohistoperoxidase procedures. For detection and titration of serum antibodies against bovine gamma globulin (BGG) this method appeared to be specific and sensitive. One advantage is that no special instruments are needed. As an example of diagnostic applicability the system was successfully used for demonstration of antibodies in human serum containing antibodies against the trematode Schistosoma mansoni. At the microscopical level the technique is suitable for study of basic problems. Microspectrophotometric absorbance scanning of the beads revealed that the method can provide quantitative information, and is probably capable of quantifying stoichiometric relations in immunological reactions.
D000613:Aminobutyrates; D000818:Animals; D000906:Antibodies / Q000032:analysis; D000941:Antigens; D002417:Cattle / Q000276:immunology; D003488:Cyanogen Bromide; D006041:Goats / Q000276:immunology; D006386:Hemagglutination Tests; D006801:Humans; D007106:Immune Sera; D010544:Peroxidases; D011817:Rabbits; D012550:Schistosoma mansoni / Q000276:immunology; D012685:Sepharose; D012698:Serologic Tests / Q000379:methods*; D013053:Spectrophotometry; D013194:Staining and Labeling; D013552:Swine / Q000276:immunology; D005719:gamma-Globulins
D000613:Aminobutyrates; D000906:Antibodies; D000941:Antigens; D007106:Immune Sera; D005719:gamma-Globulins; D012685:Sepharose; D010544:Peroxidases; D003488:Cyanogen Bromide