title: Proteinase C (carboxypeptidase Y) mutant of yeast.
abstract: A mutant of yeast lacking proteinase C (carboxypeptidase Y) activity has been found by using a histochemical stain to screen mutagenized colonies. This defect segregates 2:2 in meiotic tetrads. Cell extracts lacked the esterolytic, amidase, and proteolytic activities associated with proteinase C. The absence of proteinase C does not affect mitotic growth and has no obvious effect on the formation of viable ascospores or meiotic segregation. The mutant grows on peptides known to be cleaved by proteinase C in vitro. This finding is consistent with the idea that other enzymes exist in vivo with overlapping substrate specificities.
mesh_terms: D000813:Anilides / Q000378:metabolism; D002268:Carboxypeptidases / Q000032:analysis / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002364:Caseins / Q000378:metabolism; D002729:Chloromercuribenzoates / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D008540:Meiosis; D009154:Mutation; D010664:Phenylmethylsulfonyl Fluoride / Q000494:pharmacology; D013172:Spores, Fungal; D013194:Staining and Labeling; D015003:Yeasts / Q000201:enzymology* / Q000254:growth & development
chemical_list: D000813:Anilides; D002364:Caseins; D002729:Chloromercuribenzoates; D004791:Enzyme Inhibitors; D010664:Phenylmethylsulfonyl Fluoride; D002268:Carboxypeptidases