Peripheral blood T and B cell characteristics in a patient with severe combined immune deficiency (SCID) maintained in a gnotobiotic environment.
Peripheral blood lymphocytes obtained at 24-30 months after birth from a male with X-linked severe combined immune deficiency maintained in a gnotobiotic environment were characterized by T and B cell surface markers. A high proportion (55-80 percent) of circulating lymphocytes bore surface IgM as detected by direct immunofluorescence. A receptor for the activated C3 complement component was detected on 27-47 percent of his lymphocytes. Only 4-12 percent of the peripheral blood lymphocytes formed spontaneous rosettes with sheep erythrocytes (E-R). In general, no blastogenesis was detected in lymphocyte cultures stimulated with pokeweed mitogen or phytohemagglutinin although transient slightly positive responses to both mitogens were occasionally observed. Incubation of lymphocytes with bovine thymosin Fraction V did not increase the percentage of E-R nor induce lymphocyte blastogenesis in the presence of phytohemagglutinin.
D001402:B-Lymphocytes / Q000276:immunology*; D002462:Cell Membrane / Q000276:immunology; D002675:Child, Preschool; D000939:Epitopes; D005856:Germ-Free Life; D006801:Humans; D007104:Immune Adherence Reaction; D007075:Immunoglobulin M; D007153:Immunologic Deficiency Syndromes / Q000235:genetics / Q000276:immunology*; D037102:Lectins / Q000494:pharmacology; D008213:Lymphocyte Activation; D008297:Male; D012730:Sex Chromosomes; D013601:T-Lymphocytes / Q000276:immunology*; D013947:Thymosin / Q000494:pharmacology
D000939:Epitopes; D007075:Immunoglobulin M; D037102:Lectins; D013947:Thymosin