[Azoindoxyl methods for the histochemical investigation of hydrolases. I. Lactase (lactase-beta-glucosidase complex) (author's transl)].
An azoindozyl method for the histochemical demonstration of lactase (lactase-beta-glucosidase complex) is described. The incubation medium consists of 5 mg 5-Br-4-Cl-3-indolyl-beta-D-fucoside (dissolved in 0.5 ml N,N-dimethylformamide) and 0.02 ml hexazotized prosaniline in 10 ml 0.1 M citric acid phosphate buffer, pH 6-6.5. By means of this method lactase can be exactly localized in the brush border of the enterozytes in the jejunum of suckling rats. Compared to the corresponding indigogenic method the azoindoxyl reaction proceeds faster and the reaction product is often precipitated more precisely.
D000818:Animals; D003979:Diazonium Compounds; D005696:Galactosidases / Q000032:analysis*; D005959:Glucosidases / Q000032:analysis; D006651:Histocytochemistry; D007211:Indoles; D007413:Intestinal Mucosa / Q000201:enzymology; D007583:Jejunum / Q000201:enzymology; D009097:Multienzyme Complexes / Q000032:analysis; D051381:Rats; D012394:Rosaniline Dyes; D013194:Staining and Labeling
D003979:Diazonium Compounds; D007211:Indoles; D009097:Multienzyme Complexes; D012394:Rosaniline Dyes; D005696:Galactosidases; D005959:Glucosidases
Azoindoxylverfahren zum histochemischen Hydrolasennachweis. I. Lactase (Lactase-beta-glucosidase-Komplex)