[Identification of proteins by immunochemical methods after electrophoretic runs on different supports (author's transl)].
The determination of the location of serum proteins after electrophoresis on various supports and after immunoelectrophoresis has been achieved by immunochemical methods with specific antisera. The present study describes the technique, gives details of various identified fractions and presents some figures. The method allows us to study the constitution of the "beta-gamma block" observed on paper and acetate supports after electrophoresis of cirrhotic sera and to superimpose the patterns of apparently distinct fractions on polyacrylamide gel.
D001609:Beta-Globulins / Q000032:analysis; D001797:Blood Protein Electrophoresis / Q000379:methods*; D001798:Blood Proteins / Q000032:analysis*; D004587:Electrophoresis, Agar Gel / Q000379:methods; D004588:Electrophoresis, Cellulose Acetate / Q000379:methods; D004590:Electrophoresis, Paper / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006801:Humans; D007122:Immunoelectrophoresis / Q000379:methods*; D005719:gamma-Globulins / Q000032:analysis
D001609:Beta-Globulins; D001798:Blood Proteins; D005719:gamma-Globulins
Méthodes immunochimiques d'identification de protéines après migration électrophorétique sur différents supports