Age-dependent variations of lysosomal enzymes in human liver.
The specific activities, the Km values, and the elution patterns on DEAE 52 and Sephadex G-150 columns of six lysosomal enzymes in human liver during development were studied. The levels of total beta-D-glucuronidase and N-acetyl-beta-D-glucosaminidase activities in childhood liver were higher than those in fetal liver. The Km values of beta-D-glucuronidase and beta-D-galactosidase in fetal liver were about ten times higher than those in childhood liver. The elution patterns on DEAE 52 of beta-D-glucuronidase and on Sephadex G-150 of alpha-D- and beta-D-galactosidases changed with aging. It is suggested that lysosomal enzymes related to degradation of gangliosides and glycosaminoglycans change during development.
D000118:Acetylglucosaminidase / Q000032:analysis; D000367:Age Factors; D002648:Child; D002675:Child, Preschool; D005333:Fetus / Q000201:enzymology; D005696:Galactosidases / Q000032:analysis; D005865:Gestational Age; D005959:Glucosidases / Q000032:analysis; D005966:Glucuronidase / Q000032:analysis; D006026:Glycoside Hydrolases / Q000032:analysis*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007223:Infant; D008099:Liver / Q000166:cytology / Q000201:enzymology*; D008247:Lysosomes / Q000201:enzymology*; D008361:Mannosidases / Q000032:analysis
D005696:Galactosidases; D005959:Glucosidases; D006026:Glycoside Hydrolases; D008361:Mannosidases; D005966:Glucuronidase; D000118:Acetylglucosaminidase