Mouse MSV transformed cells resistant to 8-azaguanine.
Mouse cells transformed by murine sarcoma virus were made resistant to 8-azaguanine. Resistant cells and cell clones isolated from them were deficient in hypoxanthine-guanine phosphoribosyl transferase (HGPRT) activity. They did not grow in HATG medium, did not incorporate labeled hypoxanthine, and had negligible HGPRT activity. The resistance was genetically stable. The resistant cells were hyperdiploid and contained telocentric chromosomes only. The resistant cells as well as the progenitor cells were slightly tumorigenic in mice, the plating efficiency in soft agar was very low. The parental cells and aza-G resistant cells produced C-type viral particles having RNA-dependent DNA polymerase activity. The resistance to aza-G did not influenced the expression of murine sarcoma virus genome in cells. The resistant cells are suitable for preparation of cell hybrids.
D000818:Animals; D001375:Azaguanine / Q000494:pharmacology*; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D004279:DNA, Viral; D004351:Drug Resistance; D017988:Gammaretrovirus; D007041:Hypoxanthine Phosphoribosyltransferase / Q000378:metabolism; D007042:Hypoxanthines / Q000378:metabolism; D009374:Neoplasms, Experimental / Q000209:etiology; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D012190:Retroviridae / Q000254:growth & development; D009053:Sarcoma Viruses, Murine / Q000254:growth & development; D014779:Virus Replication
D004279:DNA, Viral; D007042:Hypoxanthines; D007041:Hypoxanthine Phosphoribosyltransferase; D012194:RNA-Directed DNA Polymerase; D001375:Azaguanine