Analysis of cysteinyldopas, dopa, dopamine, noradrenaline and adrenaline in serum and urine using high-performance liquid chromatography and electrochemical detection.
The catecholic amino acids, dopa, 2-S- and 5-S-cysteinyldopa, and 2,5-S,S-dicysteinyldopa were determined qualitatively in serum from patients with malignant melanoma by reversed-phase high-performance liquid chromatography, using electrochemical detection. In urine the catecholamines dopamine, noradrenaline and adrenaline were also determined qualitatively, as well as the above-mentioned compounds, in a single chromatographic run. The conditions were optimized by changing the pH of the mobile phase and by the addition of methanesulphonic acid. A comparison was made between the performance of four commercial reversed-phase packing materials containing chemically bonded octadecyl groups, using a standard mixture of catecholic amino acids. The influence of ionic strength, pH and amount of methanesulphonic acid on retention was investigated.
D000327:Adsorption; D000537:Aluminum Oxide; D002395:Catecholamines / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D003548:Cysteinyldopa / Q000032:analysis; D004295:Dihydroxyphenylalanine / Q000032:analysis; D004298:Dopamine / Q000032:analysis; D004563:Electrochemistry; D004837:Epinephrine / Q000032:analysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008722:Methods; D009638:Norepinephrine / Q000032:analysis; D009994:Osmolar Concentration
D002395:Catecholamines; D007202:Indicators and Reagents; D003548:Cysteinyldopa; D004295:Dihydroxyphenylalanine; D000537:Aluminum Oxide; D004298:Dopamine; D009638:Norepinephrine; D004837:Epinephrine