Cytochemistry of oxidoreductases in spermatozoa: the technique revisited.
A revised cytochemical procedure for demonstrating sperm oxidoreductases is presented in an attempt to standardize its use in comparative and experimental studies. Its detailed description includes improved incubation techniques, preparation of media, preservation of cytochemical activity on smears and evaluation of cytochemical preparations. Special attention is paid to the competing activities which may affect the specificity of the results.
D000818:Animals; D005188:False Negative Reactions; D005562:Formazans / Q000378:metabolism; D006651:Histocytochemistry / Q000379:methods; D006801:Humans; D008297:Male; D008928:Mitochondria / Q000201:enzymology; D009246:NADH Tetrazolium Reductase / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D009580:Nitroblue Tetrazolium / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism*; D012662:Semen Preservation; D013045:Species Specificity; D013094:Spermatozoa / Q000201:enzymology*
D005562:Formazans; D009580:Nitroblue Tetrazolium; D009249:NADP; D010088:Oxidoreductases; D009246:NADH Tetrazolium Reductase; D009247:NADH, NADPH Oxidoreductases