Analysis of antigenic determinants of structural polypeptides of avian type C tumor viruses.
Radioimmunoassays were developed for the 19,000, 15,000, and 12,000 molecular weight polypeptides of avian myeloblastosis virus and for the 19,000 and 12,000 polypeptides of RAV-0, a subgroup E avian tumor virus. Each polypeptide was shown to possess both group- and type-specific antigenic determinants, in contrast to the 27,000 mol wt polypeptide, which contained only group-specific determinants. The corresponding low-molecular-weight polypeptides of subgroup A, B, and E viruses were shown to be immunologically indistinguishable. The findings that low-molecular-weight polypeptides of subgroup C and D viruses reacted very differently in immunoassays for the respective polypeptides of avian myeloblastosis virus or RAV-0 suggest that subgroups C and D may have evolved differently form subgroups A, B, and E.
D000818:Animals; D001354:Avian Leukosis Virus / Q000276:immunology*; D009189:Avian Myeloblastosis Virus / Q000276:immunology; D001358:Avian Sarcoma Viruses / Q000276:immunology; D002642:Chick Embryo; D002700:Chloramines; D002850:Chromatography, Gel; D046508:Culture Techniques; D000939:Epitopes; D005347:Fibroblasts; D007457:Iodine Radioisotopes; D008970:Molecular Weight; D010455:Peptides / Q000276:immunology*; D011863:Radioimmunoassay; D012190:Retroviridae / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology*
D002700:Chloramines; D000939:Epitopes; D007457:Iodine Radioisotopes; D010455:Peptides; D014764:Viral Proteins