Positive controls for fungal detection in tissue sections.
Lyophilized Histoplasma capsulatum, Coccidioides immitis, Blastomyces dermatitidis and Aspergillus fumigatus are rehydrated, evenly dispersed, and made into smears using a bacteriological loop and alcohol-cleaned slides. Smears are air dried for 15 minutes, fixed in concentrated formaldehyde fumes for 30 minutes, postfixed for one hour in ten per cent buffered formaldehyde, and stored at room temperature. These preparations are used as positive controls for the Grocott stain as recommended for confirmation of fungi detected in tissue sections.
D001232:Aspergillus fumigatus / Q000302:isolation & purification; D001758:Blastomyces / Q000302:isolation & purification; D003045:Coccidioides / Q000302:isolation & purification; D005658:Fungi / Q000302:isolation & purification*; D006652:Histological Techniques; D006658:Histoplasma / Q000302:isolation & purification; D013194:Staining and Labeling