X-ray microanalysis of zinc and calcium in ultrathin sections of human sperm cells using the pyroantimonate technique.
X-ray microanalysis of pyroantimonate-fixed sperm cells indicates the retention of calcium and zinc subcellularly in similar proportions to air dried cells. The ultrastructure is well preserved and is corelated with the analysis. Sodium, potassium and chlorine are all removed during the fixation. CAlcium and zinc are found present intracellularly both in association with and independent of antimonate precipitation. There thus appears to be a varying degree of binding of those elements subcellularly, precipitation occurring where binding is reduced.
D002118:Calcium / Q000032:analysis*; D004577:Electron Probe Microanalysis; D006651:Histocytochemistry; D006801:Humans; D008297:Male; D009928:Organ Specificity; D013094:Spermatozoa / Q000032:analysis* / Q000648:ultrastructure; D013194:Staining and Labeling; D015032:Zinc / Q000032:analysis*
D015032:Zinc; D002118:Calcium