Isolation of the catalytic subunits of cyclic AMP-dependent protein kinases from different mammalian tissues on the basis of charge differences of their subunits.
Both, the experimental data and a literature survey presented reveal common charge differences in the subunit composition of cAMP-dependent protein kinases from a variety of mammalian tissues. In general, the holoenzymes (type I and II) focus at pH 4.5-5.5, the cAMP-binding regulatory subunit below pH 4, whereas catalytic subunits are found at pH 6.7-9.1. For the purification of the catalytic subunits, the anionic holoenzymes of a variety of rat and rabbit organs as well as mouse and human-derived tissue culture cells were adsorbed to DEAE-cellulose. The catalytic subunit from the particular isoenzyme were selectively eluted at an appropriate ionic strength depending on the isoenzyme elution pattern by small amounts of cAMP. Extraction of tissues with Triton X-100 increased enzyme yield. Improved elution of the enzyme was accomplished by 8-(4-aminobutyl)amino-cAMP instead of cyclic AMP. Carboxymethyl-cellulose chromatography leads to both, concentration and, if necessary, to further purification of the enzyme.
D000818:Animals; D001921:Brain / Q000201:enzymology; D002417:Cattle; D000242:Cyclic AMP / Q000494:pharmacology; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D046911:Macromolecular Substances; D009132:Muscles / Q000201:enzymology; D009928:Organ Specificity; D011494:Protein Kinases / Q000302:isolation & purification* / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D013045:Species Specificity
D007527:Isoenzymes; D046911:Macromolecular Substances; D000242:Cyclic AMP; D011494:Protein Kinases