Binding characteristics of 3H-prazosin to rat brain alpha-adrenergic receptors.
3H-Prazosin binding to membranes from rat brain is saturable, Bmax, 77 fmol/mg protein, of high affinity, KD, 0.28 nM and with a drug specificity indicating that it labels alpha-adrenergic receptors. The ranking of alpha-antagonists for these binding sites suggests that 3H-prazosin may be useful in identifying a subpopulation of alpha-receptors (alpha1) in the central nervous system.
D000316:Adrenergic alpha-Agonists / Q000378:metabolism; D000317:Adrenergic alpha-Antagonists / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D008566:Membranes / Q000378:metabolism; D011224:Prazosin / Q000378:metabolism*; D011485:Protein Binding; D011799:Quinazolines / Q000378:metabolism*; D051381:Rats; D011941:Receptors, Adrenergic / Q000378:metabolism*; D011942:Receptors, Adrenergic, alpha / Q000378:metabolism*
D000316:Adrenergic alpha-Agonists; D000317:Adrenergic alpha-Antagonists; D011799:Quinazolines; D011941:Receptors, Adrenergic; D011942:Receptors, Adrenergic, alpha; D011224:Prazosin