Endogenous ecotropic mouse type C viruses deficient in replication and production of XC plaques.
Endogenous ecotropic type C viruses were induced by iodedeoxyuridine from nontransformed and chemically or spontaneously transformed clones of the C3H/10T1/2 cell line. Viruses produced by cells of certain transformed clones were N-tropic and formed large XC plaques. In contrast, viruses produced by nontransformed C3H/10T1/2 cells were not detectable in the XC plaque test. These XC- viruses infected mouse cells with high efficiency, as shown by the induction of murine leukemia virus group-specific antigens in infected cells, but virus production, as determined by DNA polymerase-containing particles, was extremely low. Upon growth in certain mouse cells these replication-deficient, XC(-) viruses converted to type C viruses that were similar in XC assays to N-tropic AKR virus (XC+).
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002642:Chick Embryo; D003673:Defective Viruses / Q000201:enzymology / Q000254:growth & development* / Q000276:immunology; D007065:Idoxuridine / Q000494:pharmacology; D009052:Leukemia Virus, Murine / Q000201:enzymology / Q000254:growth & development* / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D012194:RNA-Directed DNA Polymerase / Q000096:biosynthesis; D012190:Retroviridae / Q000201:enzymology / Q000254:growth & development* / Q000276:immunology; D014779:Virus Replication
D000956:Antigens, Viral; D012194:RNA-Directed DNA Polymerase; D007065:Idoxuridine