title: [Cytochemical studies in megakaryocytes in hematologic diseases].
abstract: Bone marrow smears of 48 patients consisting of 12 normal cases, 36 patients with different haematological diseases-among them 9 cases of idiopathic thrombopenia, 4 cases of polycythaemia, and 9 cases of Hodgkin's disease - were examined cytochemically. Acid phosphatase, unspecific esterases, naphthol-AS-D-chloroacetate esterase, peroxydase, and leucin-aminopeptidase were represented; in addition the PAS reaction, fastgreen staining at pH 1.1, methyl-green pyronin staining and the lipid representation with Sudan black B were carried out. Besides those responses known from literature the different behaviour of acid megacaryocyte phosphatase in different haematological diseases must be particularly emphasized from all reactions.
mesh_terms: D000135:Acid Phosphatase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000626:Aminopeptidases / Q000097:blood; D001798:Blood Proteins / Q000032:analysis; D004950:Esterases / Q000097:blood; D006402:Hematologic Diseases / Q000473:pathology*; D006689:Hodgkin Disease / Q000378:metabolism; D006801:Humans; D008533:Megakaryocytes / Q000201:enzymology*; D010544:Peroxidases / Q000097:blood; D011087:Polycythemia Vera / Q000378:metabolism; D011134:Polysaccharides / Q000032:analysis; D013921:Thrombocytopenia / Q000378:metabolism
chemical_list: D001798:Blood Proteins; D011134:Polysaccharides; D010544:Peroxidases; D004950:Esterases; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D000626:Aminopeptidases
vernacular_title: Zytochemische Untersuchungen der Megakaryozyten bei hämatologischen Erkrankungen