Lactoglobulins (goat) in the immunofluorescence staining of parainfluenza virus type 2 in L-132 cells.
Instillation of parainfluenza virus type 2 in the mammary gland of the goat resulted in the appearance of specific and high titered antibodies in the milk. The globulin fraction of the milk was separated and conjugated with fluorescein isothiocyanate. These conjugated antibodies could be successfully used in the specific staining of the homologous virus in infected L-132 cells. Therefore, milk from such immunized animals represents a rich and convenient source of specific antibodies for use in immunofluorescence and other serological tests.
D000818:Animals; D000914:Antibodies, Viral / Q000302:isolation & purification*; D005455:Fluorescent Antibody Technique / Q000379:methods*; D006041:Goats; D002479:Inclusion Bodies / Q000276:immunology; D007739:L Cells / Q000276:immunology*; D007782:Lactoglobulins / Q000276:immunology*; D008321:Mammary Glands, Animal; D008892:Milk / Q000382:microbiology; D010223:Parainfluenza Virus 2, Human / Q000302:isolation & purification*; D018184:Paramyxoviridae Infections / Q000662:veterinary*; D018108:Respirovirus / Q000302:isolation & purification*; D013194:Staining and Labeling
D000914:Antibodies, Viral; D007782:Lactoglobulins