Anti-receptor antibody. I. Isolation and characterization of the immunoglobulin receptor for phosphorylcholine.
We have demonstrated a phosphorylcholine-binding protein in lysates from radioiodinated splenocytes of immunized mice. Immunoprecipitation with anti-receptor antibody of lysates from splenocytes obtained from animals undergoing a primary response or from mice immunized 4 to 6 months earlier with antigen demonstrated that this protein contained H and L chains. Therefore, we have isolated an antigen-specific receptor from the surface of spleen cells from both immunized and "memory" animals. This receptor Ig comprises approximately 10% of total cell surface Ig.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D001666:Binding Sites, Antibody; D002462:Cell Membrane / Q000276:immunology; D002794:Choline / Q000031:analogs & derivatives / Q000276:immunology; D000939:Epitopes; D005260:Female; D006241:Haptens; D007117:Immunization, Secondary; D007140:Immunoglobulin Fab Fragments / Q000302:isolation & purification; D007143:Immunoglobulin Heavy Chains / Q000302:isolation & purification; D007136:Immunoglobulins / Q000302:isolation & purification*; D007156:Immunologic Memory; D007457:Iodine Radioisotopes; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009194:Myeloma Proteins; D009943:Organophosphorus Compounds / Q000276:immunology; D013154:Spleen / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D000939:Epitopes; D006241:Haptens; D007140:Immunoglobulin Fab Fragments; D007143:Immunoglobulin Heavy Chains; D007136:Immunoglobulins; D007457:Iodine Radioisotopes; D009194:Myeloma Proteins; D009943:Organophosphorus Compounds; D002794:Choline