A high resolution study of the DNA replication patterns of chinese hamster chromosomes using sister chromatid differential staining technique.
Chinese hamster cells were grown for 1+ and 2+ cell cycles in the presence of BrdU and then treated by the sister chromatid differential staining technique (SCD). Those regions of a chromosome which had replicated twice in the presence of BrdU were pale staining and by selecting appropriate metaphase cells an accurate reconstruction of the DNA synthetic patterns was possible. A direct correlation between the staining intensity of the G bands and the order in which they replicate was found. Dark staining G bands were always the last region of a chromosome to replicate while G negative bands were first. It is concluded that each G band may be a cluster of replicons capable of initiating DNA synthesis simultaneously.
D000818:Animals; D001973:Bromodeoxyuridine / Q000378:metabolism; D002842:Chromatids / Q000378:metabolism; D002875:Chromosomes / Q000378:metabolism*; D006224:Cricetinae; D004247:DNA / Q000096:biosynthesis; D004261:DNA Replication; D008938:Mitosis; D013194:Staining and Labeling; D013997:Time Factors
D004247:DNA; D001973:Bromodeoxyuridine