The effect of anti-alpha2-macroglobulin on K-cell cytolysis and T- and B-cell rosette formation.
The effect of antisera to human alpha2-macroglobulin (alpha2M) on the ability of human peripheral blood lymphoid cells to lyse antibody coated target cells (exert a K-cell effect) and form T(E) and B(EAC) rosettes has been investigated. Pretreatment of lymphoid cells with these antisera inhibited their K-cell activity but had no effect on their capacity to form T or B rosettes. The inhibitory activity of the anti-alpha2M sera was always reduced following absorption on a CnBr column to which human alpha2M had been coupled. The absorbed antibody, recovered by acid elution reacted strongly with human alpha2M in gel diffusion precipitin analysis and caused a highly significant inhibition of K-cell cytotoxicity. It was further shown that the inhibitory activity of the anti-alpha2M was localized exclusively in the IgG fraction and was greatly reduced following pepsin digestion.
D000906:Antibodies; D001402:B-Lymphocytes / Q000276:immunology*; D002852:Chromatography, Ion Exchange; D003601:Cytotoxicity Tests, Immunologic; D006461:Hemolysis; D006801:Humans; D007104:Immune Adherence Reaction; D005779:Immunodiffusion; D007074:Immunoglobulin G; D066298:In Vitro Techniques; D013601:T-Lymphocytes / Q000276:immunology*; D000511:alpha-Macroglobulins / Q000276:immunology*
D000906:Antibodies; D007074:Immunoglobulin G; D000511:alpha-Macroglobulins