title: Growth of infective forms of Trypanosoma (T.) brucei on buffalo lung and Chinese hamster lung tissue culture cells.
abstract: Infective cultures of Trypanosoma (T.) brucei (strain 427) have been initiated and maintained on Chinese hamster lung tissue culture cells and buffalo lung tissue culture cells. By changing daily one-third of the RPMI-1640 plus 20% fetal bovine serum medium, the cell numbers can be maintained at 2--4 X 10(6) cells/ml. The cultured trypanosomes on these two tissue culture cell types were infective to mice and morphologically similar to bloodstream slender trypomastigotes in having a subterminal kinetoplast and a surface coat. In addition, they possessed the L-alpha-glycerophosphate oxidase, the predominant steady state terminal oxidase identified in bloodstream trypomastigotes.
mesh_terms: D000818:Animals; D002020:Buffaloes; D006224:Cricetinae; D003412:Cricetulus; D046508:Culture Techniques; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D008168:Lung; D014346:Trypanosoma brucei brucei / Q000201:enzymology / Q000254:growth & development* / Q000472:pathogenicity
chemical_list: D005993:Glycerolphosphate Dehydrogenase