Demonstration of a tumor-associated surface antigen in Marek's disease.
Surface antigenic markers were detected on three classes of Marek's disease (MD) tumor cells, i.e., MD lymphoma cells, cultured cells of the MSB-1 lymphoblastoid cell line, and JMV lymphoblastic leukemia cells, by indirect membrane immunofluorescent staining with serum from chickens immunized with JMV cells or from rabbits immunized with MSB-1 cells. This surface antigen was not detected on normal chicken lymphocytes, RPL-16 tumor cells (tranedormed by an avian RNA virus, or MD virus-infected fibroblasts that were positive for viral membrane antigen (MA). Furthermore, the surface antigen appeared unrelated to embryonic or histocompatibility antigens. This antigen is provisionally designated as a Marek's disease tumor-associated surface antigen (MATSA). The MATSA's on JMV, MSB-1 and MD lymphoma cells were related but not identical as demonstrated by antiserum titration, absorption and blocking tests with homologous and heterologous systems.
D000704:Analysis of Variance; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D000937:Antigen-Antibody Reactions; D000951:Antigens, Neoplasm / Q000032:analysis*; D002462:Cell Membrane / Q000276:immunology*; D002645:Chickens; D003289:Convalescence; D000939:Epitopes; D005455:Fluorescent Antibody Technique; D007106:Immune Sera / Q000302:isolation & purification; D066298:In Vitro Techniques; D008221:Lymphoid Tissue / Q000166:cytology; D008380:Marek Disease / Q000276:immunology*; D009374:Neoplasms, Experimental / Q000276:immunology; D009500:Neutralization Tests; D011817:Rabbits / Q000276:immunology; D013154:Spleen / Q000276:immunology; D013160:Splenic Neoplasms / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology
D000914:Antibodies, Viral; D000951:Antigens, Neoplasm; D000939:Epitopes; D007106:Immune Sera