title: Comparison of four techniques for the routine diagnosis of Trichomonas vaginalis infection.
abstract: Specimens from 495 patients attending Johannesburg hospitals and family planning clinics were examined for Trichomonas vaginalis by microscopy of Giemsa (GS), Papanicolaou (PAP), and acridine-orange (AO) stained smears, and by culture in Feinberg-Whittington medium. Culture, Pap and GS stained smears from vaginal swabs yielded fewer positives than AO stained smears. Although Pap-stained cytological smears gave the highest number of positives, in 30% of these cases the presence of T.vaginalis could not be confirmed by examination of vaginal swabs. Of the positive AO-stained smears, 93% were also positive by at least one other technique.
mesh_terms: D000166:Acridines; D001399:Azure Stains; D002582:Cervix Mucus / Q000382:microbiology; D003470:Culture Media; D005260:Female; D006801:Humans; D008722:Methods; D065006:Papanicolaou Test; D013194:Staining and Labeling; D014247:Trichomonas Vaginitis / Q000175:diagnosis* / Q000382:microbiology; D014246:Trichomonas vaginalis / Q000302:isolation & purification*; D014626:Vaginal Smears / Q000379:methods
chemical_list: D000166:Acridines; D001399:Azure Stains; D003470:Culture Media