Double-antibody enzyme immunoassay applied to human alpha 1-fetoprotein.
We report the development of a double-antibody system for enzyme immunoassay of human alpha 1-fetoprotein. Equilibrium and sequential saturation procedures were evaluated and compared with a similar double-antibody radioimmunoassay. With our method, 3 mug of alpha 1-fetoprotein per liter can be detected. The dose-response curve covers a 100-fold range of analyte concentrations. Within-run and between-run coefficients of variation are respectively 6.9% and 10.0%. Analytical recovery of added antigen is 99.5 +/- 10.6%. Results are routinely obtained in 24 h but can be obtained in less than 8 h. Alpha 1-fetoprotein was measured in a variety of clinical samples, including sera from 488 pregnant women. The advantages of the present method over radioimmunoassay and other enzyme immunoassay procedures are discussed.
D000469:Alkaline Phosphatase; D000653:Amniotic Fluid / Q000032:analysis; D000818:Animals; D002417:Cattle; D005260:Female; D005312:Fetal Blood / Q000032:analysis; D005326:Fetal Proteins / Q000032:analysis*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D007413:Intestinal Mucosa / Q000201:enzymology; D008107:Liver Diseases / Q000097:blood; D011247:Pregnancy; D011863:Radioimmunoassay; D013696:Temperature; D000509:alpha-Fetoproteins / Q000032:analysis*
D005326:Fetal Proteins; D000509:alpha-Fetoproteins; D000469:Alkaline Phosphatase