Influence of indicating enzyme reaction on apparent creatine kinase activity creatine kinase in serum, VII.
Comparison of the indicative systems yeast glucose-6-phosphate dehydrogenase/NADP+, leuconostoc glucose-6-phosphate dehydrogenase/NADP+ and leuconostoc glucose-6-phosphate dehydrogenase/NAD+ showed excellent correlation and no differences in apparent creatine kinase activity with the two methods using NADP+. By using NAD+ with the leuconostoc enzyme enzyme relative recovery of apparent creatine kinase activity is lower due to interference of other serum constituents. The mean value of the relative differences versus methods using NADP+ was 5.8% in our experiments.
D003402:Creatine Kinase / Q000097:blood*; D005954:Glucosephosphate Dehydrogenase; D007202:Indicators and Reagents; D007934:Leuconostoc; D009249:NADP
D007202:Indicators and Reagents; D009249:NADP; D005954:Glucosephosphate Dehydrogenase; D003402:Creatine Kinase