Fluorometric assay of the arylsulphatases in human urine.
A method is described which differentiates arylsulphatase A and arylsulphatase B in human urine. 4-Methylumbelliferyl sulphate serves as the substrate, and silver ions are used to inhibit arylsulphatase A activity. Using fresh urine it is possible to obtain separate values for arylsulphatase A and B when both are present, thus providing a diagnostic test for metachromatic leucodystrophy.
D002553:Cerebroside-Sulfatase / Q000652:urine*; D002803:Chondro-4-Sulfatase / Q000652:urine*; D002852:Chromatography, Ion Exchange; D004796:Clinical Enzyme Tests; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007966:Leukodystrophy, Metachromatic / Q000175:diagnosis*; D013050:Spectrometry, Fluorescence / Q000379:methods; D013429:Sulfatases / Q000652:urine*
D013429:Sulfatases; D002553:Cerebroside-Sulfatase; D002803:Chondro-4-Sulfatase