Non-specific interference of certain components of tissue culture media with the radioimmunoassay of rat alpha-foetoprotein.
Interferences of 'Williams' tissue culture medium, used for cultivating rat hepatocytes, upon rat alpha-foetoprotein (AFP) radioimmunoassay have been investigated; they are not due to foetal calf serum proteins which are added as growth factor and can be abolished by dialysis which appears to be necessary for the distinction between AFP non-producer and low-producer cell lines. Of the three major groups of non-mineral components examined, amino acid solution played a major role; when individual amino acids were examined using the double antibody technique, arginine was found to interfere predominantly; its dose-response curve was parallel to that of rat AFP, which confirmed that an immunological identity between two substances cannot be established on the basis of parallelism as the only criterion.
D000818:Animals; D001120:Arginine / Q000494:pharmacology; D003470:Culture Media; D046508:Culture Techniques; D005326:Fetal Proteins / Q000032:analysis*; D007457:Iodine Radioisotopes; D011863:Radioimmunoassay; D051381:Rats; D000509:alpha-Fetoproteins / Q000032:analysis*
D003470:Culture Media; D005326:Fetal Proteins; D007457:Iodine Radioisotopes; D000509:alpha-Fetoproteins; D001120:Arginine