ELISA methodology for polysaccharide antigens: protein coupling of polysaccharides for adsorption to plastic tubes.
A method is described which permits the adaption of ELISA techniques for measurement of antibody against bacterial polysaccharides. First, the polysaccharides antigen is covalently bound to poly-L-lysine, using cyanuric chloride as the coupling agent. The poly-L-lysine then adsorbs to the walls of plastic tubes, thus immobilizing the polysaccharide coupled to the poly-L-lysine. The method is simple, rapid, and utilizes small amounts of polysaccharide antigen.
D000327:Adsorption; D000941:Antigens / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D007124:Immunoenzyme Techniques; D008722:Methods; D010969:Plastics / Q000378:metabolism; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D011506:Proteins; D013292:Streptococcus agalactiae; D013296:Streptococcus pneumoniae
D000941:Antigens; D010969:Plastics; D011135:Polysaccharides, Bacterial; D011506:Proteins