Antigenic and enzymatic changes in infected and transformed human diploid cells.
Human diploid fibroblasts, WI-38, were infected with various agents and the levels of lysosomal enzymes determined by immunochemical quantitation. Esterase levels were raised by mumps virus and Toxoplasma gondii infection. The concentration of beta-D-glucuronidase was reduced by these same agents. Beta-D-N-acetyl glucosaminidase was greatly increased in cultures infected with T. gondii and decreased by mycoplasma infection. Two cultures transformed by SV40 showed reduced levels of esterase compared with WI-38, as did one of two transformed amnion cultures. A second amnion culture and a culture of transformed Detroit 551 fibroblasts were unchanged. The levels of acid phosphatase were sharply reduced in three of the four SV40 transformed cultures tested.
D000135:Acid Phosphatase; D000650:Amnion; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D046508:Culture Techniques; D004622:Embryo, Mammalian; D004625:Embryo, Nonmammalian; D004950:Esterases; D005260:Female; D005347:Fibroblasts / Q000201:enzymology* / Q000276:immunology; D005966:Glucuronidase; D006596:Hexosaminidases; D006801:Humans; D007106:Immune Sera; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007527:Isoenzymes; D008168:Lung; D009107:Mumps / Q000276:immunology; D009109:Mumps virus / Q000276:immunology; D009174:Mycoplasma / Q000276:immunology; D013539:Simian virus 40; D012867:Skin; D013194:Staining and Labeling; D014122:Toxoplasma / Q000276:immunology
D007106:Immune Sera; D007527:Isoenzymes; D004950:Esterases; D000135:Acid Phosphatase; D006596:Hexosaminidases; D005966:Glucuronidase