An autoradiographic study of human blood basophils.
The aim of this paper is the selective visualization of human blood basophils in autoradiographs. [(3)H]Thymidine-labelled basophils in buffy coat smears were fixed in methanol-formaldehyde follwed by 5-aminoacridine hydrochloride and stained with basic Aldehyde Fuchsin prior to autoradiographic processing. The technique described represents a simple method for quantitative autoradiographic studies on basophil kinetics and the interaction of these cells with IgE-mediated atopias.
D000166:Acridines; D001345:Autoradiography; D001491:Basophils / Q000378:metabolism / Q000648:ultrastructure*; D006651:Histocytochemistry; D006801:Humans; D007073:Immunoglobulin E; D008722:Methods; D013194:Staining and Labeling; D013936:Thymidine / Q000378:metabolism
D000166:Acridines; D007073:Immunoglobulin E; D013936:Thymidine