The metabolism of N-hydroxyphentermine by rat liver microsomes.
N-Hydroxyphentermine is oxidized to 2-methyl-2-nitro-1-phenylpropane by rat liver microsome preparations. This oxidation accounts for differences noted in levels of N-hydroxyphentermine formed from phentermine in washed and unwashed microsome preparations. The reaction is inhibited by hemoglobin and catalase, whose presence in unwashed microsomes could terminate the N-oxidation of phentermine at the hydroxylamine level. The hydroxylamine-nitro-oxidation appears to be dependent on cytochrome P-450, as the reaction was induced by phenobarbital pretreatment and inhibited by carbon monoxide and 2,4-dichloro-6-phenylphenoxyethylamine.
D000818:Animals; D006454:Hemoglobins / Q000494:pharmacology; D066298:In Vitro Techniques; D008099:Liver / Q000201:enzymology; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D006899:Mixed Function Oxygenases / Q000378:metabolism; D010634:Phenobarbital / Q000494:pharmacology; D010645:Phentermine / Q000031:analogs & derivatives* / Q000378:metabolism; D051381:Rats; D013347:Subcellular Fractions / Q000201:enzymology
D006454:Hemoglobins; D010645:Phentermine; D006899:Mixed Function Oxygenases; D010634:Phenobarbital