Kinetics of elementary steps in the cytochrome P-450 reaction sequence. IV. Mechanism of the NADPH reduction reaction of cytochrome P-450LM.
The aerobic NADPH reduction of the Triton N-101 disintegrated cytochrome P-450LM system has been studied. At this organization level--the components being dispersed in solution--a first-order monophasic reaction is exhibited. Neither the complex formation of cytochrome and reductase, respectively, nor preceding diffusion is rate limiting. The initial rate follows the ratio reductase/P-450 (mole/mole) thus indicating a Michaelis-Menten type enzyme mechanism. A model treatment of the reaction fits the systems behaviour as a whole. A multiparameter equilibrium state and different specified time function equations were developed for the determination of individual step rate constants and other system parameters as well.
D000332:Aerobiosis; D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D007700:Kinetics; D008433:Mathematics; D008862:Microsomes, Liver / Q000378:metabolism*; D009249:NADP; D010084:Oxidation-Reduction; D011092:Polyethylene Glycols / Q000494:pharmacology; D011817:Rabbits
D011092:Polyethylene Glycols; D009249:NADP; D003577:Cytochrome P-450 Enzyme System