[The protective effect of hydroxyethylstarch (HES) in the cryoconservation of bone marrow of the mouse and of man (author's transl)].
The cryoprotective effect of hydroxyethylstarch has been tested in mice and in man using the spleen colony technique (CFU-S test) or the agar colony technique (CFU-C test) as viability assays for the haemopoietic stem cell. In mice we found a mean CFU-S recovery of 88.7%+/-3.9% when a rapid, and 77.8%+/-3.2% when a slow, stepwise dilution method was applied to remove the protective medium (17.5-20% hydroxyethylstarch+20% calf serum) after thawing. In man the rapid dilution after thawing gave a mean CFU-C recovery of 80% but a wide scatter.
D000362:Agar; D000818:Animals; D001681:Biological Assay; D001853:Bone Marrow / Q000187:drug effects; D001854:Bone Marrow Cells; D002455:Cell Division; D002470:Cell Survival / Q000187:drug effects; D002999:Clone Cells; D003451:Cryoprotective Agents; D006801:Humans; D006895:Hydroxyethyl Starch Derivatives / Q000494:pharmacology*; D066298:In Vitro Techniques; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D011830:Radiation Effects; D013154:Spleen / Q000528:radiation effects; D013213:Starch / Q000031:analogs & derivatives*; D014021:Tissue Preservation
D003451:Cryoprotective Agents; D006895:Hydroxyethyl Starch Derivatives; D000362:Agar; D013213:Starch
Die protektive Wirkung von Hydroxyäthylstärke bei der Kryokonservierung von Knochenmark der Maus und des Menschen