Immunogenicity of gonococcal Gc2 polysaccharide: comparative studies with pneumococcal type III polysaccharide and Salmonella typhosa Vi antigen.
A plaque assay technique was used to assess the immunogenicity of a gonococcal cell wall polysaccharide (Gc2 antigen) in BALB/c mice. The Gc2 antigen was shown to be immunogenic, and the kinetics of the response differed from that of a pneumococcal polysaccharide (SSS-III) and a polysaccharide antigen of Salmonella typhosa (Vi antigen). In addition, using antithymocyte sera, the T-lymphocyte dependency of these antigens was investigated. The immune response to the Gc2 antigen was demonstrated to be dependent on a population of helper T cells, whereas the response to SSS-III appears to be regulated by suppressor T cells. There appears to be marked differences in the immune response of mice to different bacterial polysaccharides.
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D000942:Antigens, Bacterial / Q000276:immunology; D000961:Antilymphocyte Serum / Q000494:pharmacology; D006462:Hemolytic Plaque Technique; D051379:Mice; D008807:Mice, Inbred BALB C; D009344:Neisseria gonorrhoeae / Q000276:immunology*; D010084:Oxidation-Reduction; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D012485:Salmonella typhi / Q000276:immunology*; D013296:Streptococcus pneumoniae / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D000961:Antilymphocyte Serum; D011135:Polysaccharides, Bacterial