Regulation of the induction of alkaline phosphatase in choriocarcinoma cells by sodium butyrate.
Alkaline phosphatase is induced in human choriocarcinoma cells by short-chain fatty acids, especially sodium butyrate. This fatty acid increases the phosphatase activity immediately and in a nearly linear fashion. Only phosphatase with an alkaline pH optimum is induced. Both the induced alkaline phosphatase and the basal enzyme are precipitated by antiserum against term-placental alkaline phosphatase, but the choriocarcinoma phosphatase is less stable to heating than is the term-placental enzyme. The induction of alkaline phosphatase activity requires cellular synthesis of protein, RNA and DNA. The regulation of induction probably occurs at the transcriptional level.
D000469:Alkaline Phosphatase / Q000096:biosynthesis*; D002087:Butyrates / Q000494:pharmacology; D002460:Cell Line; D002822:Choriocarcinoma / Q000201:enzymology*; D002999:Clone Cells; D004247:DNA / Q000096:biosynthesis; D004790:Enzyme Induction; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009363:Neoplasm Proteins / Q000096:biosynthesis; D011247:Pregnancy; D012313:RNA / Q000096:biosynthesis; D014158:Transcription, Genetic; D014594:Uterine Neoplasms / Q000201:enzymology*
D002087:Butyrates; D009363:Neoplasm Proteins; D012313:RNA; D004247:DNA; D000469:Alkaline Phosphatase