Antigenic determinants common to established human B-cell lines, but not shared by human T-cell lines (molt and sommer).
Rabbits were immunized with two kinds of human B-cell lines (SOMMER-B, and RPMI number 1788 cells) to get anti-B-cell sera. Sera were absorbed with human red blood cells, human liver, human brain and human T cells (MOLT-4 and SOMMER-T cells). Cytotoxicity of absorbed sera was not only tested against B cells which were used for immunization but also tested against unrelated B cells, including B35M cells derived from Burkitt lymphoma specimens. Results indicated that established human B cells had antigenic determinants which were shared with allogeneic human B cells, but not shared with non-lymphoid cells or tissues, nor with extablished human T cells.
D000042:Absorption; D000818:Animals; D000936:Antigen-Antibody Complex; D000941:Antigens / Q000008:administration & dosage; D001402:B-Lymphocytes / Q000276:immunology*; D001921:Brain / Q000276:immunology; D002460:Cell Line; D003601:Cytotoxicity Tests, Immunologic; D000939:Epitopes; D004912:Erythrocytes / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D006801:Humans; D007106:Immune Sera / Q000302:isolation & purification; D007115:Immunization Schedule; D008110:Liver Extracts / Q000378:metabolism; D008297:Male; D011817:Rabbits; D013601:T-Lymphocytes / Q000276:immunology*; D014020:Tissue Extracts / Q000378:metabolism
D000936:Antigen-Antibody Complex; D000941:Antigens; D000939:Epitopes; D007106:Immune Sera; D008110:Liver Extracts; D014020:Tissue Extracts