A rapid kinetic assay for glucose using glucose dehydrogenase.
An automated kinetic assay for the determination of glucose in blood is described. The method employs the enzyme glucose dehydrogenase in the presence of mutarotase, with nicotinamide adenine dinucleotide as hydrogen acceptor. The analytical parameters of the method are determined and the flexibility of the method in relation to sample volume and sensitivity is discussed. Finally, the method is compared with automated glucose oxidase and hexokinase procedures.
D001322:Autoanalysis; D001786:Blood Glucose / Q000032:analysis*; D002237:Carbohydrate Dehydrogenases; D004355:Drug Stability; D005948:Glucose Dehydrogenases; D005949:Glucose Oxidase; D006593:Hexokinase; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008722:Methods; D009243:NAD; D010949:Plasma / Q000032:analysis
D001786:Blood Glucose; D009243:NAD; D002237:Carbohydrate Dehydrogenases; D005948:Glucose Dehydrogenases; D005949:Glucose Oxidase; D006593:Hexokinase