[Histone-nonhistone protein complex reconstruction].
A complex of chromatin proteins composed of five histon fractions and nine nonhiston fractions and stable in 2M NaCl solution was destructed by treating with urea, changing pH and decreasing the NaCl concentration, No reconstruction of the complex was observed after the said factors has been removed and the initial solvent composition restored. Nevertheless the reconstruction was detected after dissociation of the complex by desalting in the presence of DNA. The reconstruction was successful when stimulated both dimers and tetramers, the chromatin protein complex under study cannot be reconstructed by "self-organization", the only way being through the stage of nucleoproteid formation, and is similar in this respect to some other complex protein structures of the cell.
D000818:Animals; D002417:Cattle; D002843:Chromatin / Q000648:ultrastructure*; D002868:Chromosomal Proteins, Non-Histone; D006657:Histones; D006863:Hydrogen-Ion Concentration; D046911:Macromolecular Substances; D009994:Osmolar Concentration; D011485:Protein Binding; D013950:Thymus Gland / Q000648:ultrastructure; D014508:Urea
D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D006657:Histones; D046911:Macromolecular Substances; D014508:Urea
Issledovanie rekonstruktsii kompleksa gistony--negistonovye belki.