Arylsulphatases A and B in human diploid fibroblasts: differential assay with 4-methylumbelliferylsulphate and AgNO3.
A new technique is introduced for the differential assay of arylsulphatases A and B in centrifuged homogenates of cultured human skin fibroblasts, using 4-methylumbelliferyl-sulphate as a substrate and AgNO3 as a selective inhibitor of arylsulphatase A. The method can be applied in the diagnosis of metachromatic leucodystrophy, mucopolysaccharidosis type VI and mucosulphatidosis. Normal arylsulphatase activities were found in fibroblasts derived from patients with mucopolysaccharidoses types II, III-A and IV, known to be caused by deficiencies of various other sulphatases.
D002553:Cerebroside-Sulfatase / Q000032:analysis*; D002803:Chondro-4-Sulfatase / Q000032:analysis*; D003937:Diagnosis, Differential; D004171:Diploidy; D005347:Fibroblasts / Q000201:enzymology; D006801:Humans; D006923:Hymecromone / Q000031:analogs & derivatives; D007966:Leukodystrophy, Metachromatic / Q000175:diagnosis / Q000201:enzymology; D009083:Mucopolysaccharidoses / Q000175:diagnosis / Q000201:enzymology; D009087:Mucopolysaccharidosis VI / Q000175:diagnosis / Q000201:enzymology; D012835:Silver Nitrate; D012867:Skin / Q000201:enzymology; D013429:Sulfatases / Q000032:analysis*
D006923:Hymecromone; D012835:Silver Nitrate; D013429:Sulfatases; D002553:Cerebroside-Sulfatase; D002803:Chondro-4-Sulfatase