[Steroid-transforming enzymes from microorganisms. XI. Reversibility of the dehydrogenation reaction of the steroid-1-dehydrogenase from Nocardia opaca].
Highly purified preparations of the 4-en-3-oxosteroid: (acceptor)-1-en-oxidoreductase from Nocardia opaca have been investigated in both types of reactions: 1.2-dehydrogenation of the 4-en-3-oxo-derivative and 1.2-hydrogenation of the 1.4-dien-3-oxo-derivative. It was not possible to separate the hydrogenating activity from the dehydrogenating activity by affinity chromatography, disc electrophoresis, SDS-electrophoresis, and isoelectric focusing techniques. The pure enzyme preparation is discussed as only one FAD depending protein acting in response to the system as a dehydrogenase as well as a reductase.
D000730:Androstadienes / Q000378:metabolism*; D000735:Androstenedione / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D009615:Nocardia / Q000201:enzymology*; D010088:Oxidoreductases / Q000378:metabolism*
D000730:Androstadienes; D009243:NAD; D000735:Androstenedione; D009249:NADP; D010088:Oxidoreductases; C022656:3-oxosteroid delta(1) dehydrogenase
Steroidumwandelnde Enzyme aus Mikroorganismen. XI. Reversibilität der Dehydrierungsreaktion der Steroid-1-Dehydrogenase aus Nocardia opaca.