title: Inhibition of Ca2+ uptake into fragmented sarcoplasmic reticulum by antibodies against purified Ca2+, Mg2+-dependent ATPase.
abstract: Rabbit antiserum was prepared against a partially purified Ca2+, Mg2+-dependent ATPase [EC 3.6.1.3] of the SR isolated from chicken skeletal muscle. The gamma-globulin fraction of antiserum contained antibodies which combined with the purified ATPase and the SR vesicles. Binding of the antibodies strongly inhibited active transport of Ca2+ ions into the SR, but not passive leakage of Ca2+ ions from the SR. The antibodies scarcely affected the ATPase activity.
mesh_terms: D000251:Adenosine Triphosphatases / Q000276:immunology*; D000818:Animals; D000937:Antigen-Antibody Reactions; D001693:Biological Transport, Active; D002118:Calcium / Q000378:metabolism* / Q000494:pharmacology; D002645:Chickens; D006358:Hot Temperature; D008274:Magnesium / Q000494:pharmacology; D011485:Protein Binding; D011817:Rabbits / Q000276:immunology; D012519:Sarcoplasmic Reticulum / Q000201:enzymology* / Q000276:immunology; D005719:gamma-Globulins / Q000378:metabolism
chemical_list: D005719:gamma-Globulins; D000251:Adenosine Triphosphatases; D008274:Magnesium; D002118:Calcium