Continuous monitoring of free fatty acid release from adipocytes by pH-stat titration.
A methods for direct and continuous monitoring of free fatty acid release in adipocyte suspensions is described. Using a pH-stat apparatus the protons from the released free fatty acids are continuously titrated and the accumulated amount of OH- added is monitored on a recorder against time, the slope thus indicating the rate of free fatty acid release. Sinc pH is kept constant, an incubation medium with a low buffering capacity can be used, which gives the method a high sensitivity. Under the conditions described, free fatty acid release from 5% of maximal norepinephrine stumulation of rat adipocytes can be accurately measured and the kinetics can be followed over extended periods of time.
D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D005230:Fatty Acids, Nonesterified / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007700:Kinetics; D008053:Lipid Mobilization; D008722:Methods; D003012:Microbial Collagenase; D051381:Rats
D005230:Fatty Acids, Nonesterified; D003012:Microbial Collagenase