Concanavalin A-horseradish peroxidase bridge staining of alpha-1 glycoproteins separated by isoelectric focusing on polyacrylamide gel.
The Coomassie Blue protein stain and the periodic acid-Schiff stain for glycoproteins are compared to a new method of staining glycoproteins resolved electrophoretically. The method utilizes a Concanavalin A-horseradish peroxidase sequence to visualize selectively glycoproteins with terminal or internal mannose or terminal N-acetylglucosamine. The method applied to characterization of M and Z allele products of alpha-l-antitrypsins separated by isoelectric focusing of polyacrylamide gels slabs have revealed differences in carbohydrate content of various components that were previously undetected.
D000117:Acetylglucosamine / Q000032:analysis; D001665:Binding Sites; D003208:Concanavalin A; D003720:Densitometry / Q000379:methods; D006023:Glycoproteins / Q000032:analysis*; D006735:Horseradish Peroxidase; D007202:Indicators and Reagents; D007525:Isoelectric Focusing / Q000379:methods; D008358:Mannose / Q000032:analysis; D010544:Peroxidases; D011485:Protein Binding; D013194:Staining and Labeling
D006023:Glycoproteins; D007202:Indicators and Reagents; D003208:Concanavalin A; D006735:Horseradish Peroxidase; D010544:Peroxidases; D008358:Mannose; D000117:Acetylglucosamine