Alkaline lead citrate: a selective stain for glutaraldehyde precipitates of indolamines and primary catecholamines in electron microscopy.
Test-tube experiments proved that alkaline lead citrate (Reynolds, 1963), which is generally used as an electron-opaque stain, specifically reacts with precipitates formed by glutaraldehyde and biogenic amines (indolamines, primary catecholamines). Glutaraldehyde/osmium tetroxide fixation and staining of thin sections with alkaline lead citrate is recommended as an optimal preparation method of studying the above-mentioned amines at a fine structural level.
D000313:Adrenal Medulla / Q000032:analysis / Q000648:ultrastructure; D000818:Animals; D001679:Biogenic Amines / Q000032:analysis*; D002395:Catecholamines / Q000032:analysis*; D002951:Citrates; D005976:Glutaral; D007854:Lead; D008854:Microscopy, Electron; D012701:Serotonin / Q000032:analysis; D013194:Staining and Labeling
D001679:Biogenic Amines; D002395:Catecholamines; D002951:Citrates; D007854:Lead; D012701:Serotonin; D005976:Glutaral