Studies on liberation of beta-thromboglobulin from human platelets in vitro.
A platelet specific protein, beta-thromboglobulin, is liberated during the preparation of platelet poor plasma. Using combinations of different anticoagulant and anti-platelet compounds, this release can be significantly reduced. The best results were obtained when native blood was collected as soon as possible into a mixture of EDTA, prostaglandin E1 and theophylline and maintained and processed at a temperature between 0 and 4 degrees C. These technical innovations have permitted the use of a radioimmunoassay for beta-thromboglobulin on plasma samples in clinical practice.
D000925:Anticoagulants / Q000494:pharmacology; D001609:Beta-Globulins / Q000378:metabolism*; D001792:Blood Platelets; D001793:Blood Preservation; D002498:Centrifugation; D004492:Edetic Acid / Q000494:pharmacology; D006801:Humans; D011458:Prostaglandins E / Q000494:pharmacology; D013696:Temperature; D013806:Theophylline / Q000494:pharmacology
D000925:Anticoagulants; D001609:Beta-Globulins; D011458:Prostaglandins E; D004492:Edetic Acid; D013806:Theophylline