Demonstration of membrane-bound immunoglobulin determinants and the significance of a quantitative characterization of the antisera used.
Lymphocytes from 22 healthy blood donors were tested for the presence of immunoglobulin determinants on their surfaces by indirect membrane immunofluorescence. The proportion of MemIF+ cells was, on the average, 8.7% (3-18) for IgM and 15.8% (8-28) for IgG. The small differences in the proportion of MemIF+ lymphocytes upon use of various anti-IgM sera could be attributed mainly to the different antibody concentrations. The importance of a quantitative characterization of the antisera used was pointed out.
D000328:Adult; D000888:Antibodies, Anti-Idiotypic; D000939:Epitopes; D005455:Fluorescent Antibody Technique / Q000379:methods; D006801:Humans; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008214:Lymphocytes / Q000276:immunology*; D008875:Middle Aged; D011947:Receptors, Antigen, B-Cell / Q000032:analysis*
D000888:Antibodies, Anti-Idiotypic; D000939:Epitopes; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011947:Receptors, Antigen, B-Cell