Effect of renal physicochemical milieu on stimulation of human lymphocytes by phytohemagglutinin.
The effect of changes in certain physicochemical parameters, analogous to those occurring in the kidney, on stimulation of normal human lymphocytes by phytohemagglutinin was studied. Increase in osmolality by sucrose or urea, and increases in concentration of sodium, potassium and calcium all significantly inhibited stimulation while magnesium did not. Lowering pH to 6.8 and 6.5 inhibited stimulation. Raising the pH to 8.0 had no effect bu at pH 8.4 stimulation was decreased. The effect was largely but not entirely due to decrease in viability.
D002118:Calcium / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007668:Kidney; D008213:Lymphocyte Activation; D008274:Magnesium / Q000378:metabolism; D009994:Osmolar Concentration; D010835:Phytohemagglutinins; D011188:Potassium / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D014508:Urea / Q000378:metabolism
D010835:Phytohemagglutinins; D013395:Sucrose; D014508:Urea; D012964:Sodium; D008274:Magnesium; D011188:Potassium; D002118:Calcium