[Changes in intercellular exchange intensity in an L cell culture].
Intercellular junctions permeable to fluorescein Na were studied with the aid of intracellular glass microelectrodes in the cultures of transformed mouse-embryo cells (L-strain). The degree of coupling was correlated with the cell culture density. In addition, the degree of coupling increased after short-time incubated of the cells with 1mM lanthanum. Such an incubation did not influence other features of cells: the value of the membrane potential, the character of growth or the inclusion of the vital dye neutral red. Some other factors stimulated cellular aggregation (concanavalin A, protamine, decreasing the pH) but did not influence the degree of coupling.
D000818:Animals; D002449:Cell Aggregation / Q000187:drug effects; D002450:Cell Communication / Q000187:drug effects; D002463:Cell Membrane Permeability / Q000187:drug effects; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007365:Intercellular Junctions / Q000378:metabolism; D007739:L Cells / Q000378:metabolism*; D007811:Lanthanum / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects; D051379:Mice; D008809:Mice, Inbred C3H; D009499:Neutral Red / Q000494:pharmacology; D011479:Protamines / Q000494:pharmacology; D013997:Time Factors
D011479:Protamines; D003208:Concanavalin A; D009499:Neutral Red; D007811:Lanthanum
Izmeneniia intensivnosti mezhkletochnogo obmena v kul'ture kletok L.