title: Effect of ultraviolet and gamma-radiation on Herpetomonas samuelpessoai.
abstract: A study was made about the influence of ultraviolet (UV) and gamma-radiations on Herpetomonas samuelpessoai grown either in a chemically defined or in a complex medium. Cells cultivated in defined medium were more sensitive to UV than those from complex medium, as estimated by inhibition of cellular growth. The effect of gamma-radiation, however, was independent of the media in which the cells were grown. Both radiations interfere with the plasma membrane as analysed by parameters such as excretion of cellular material and concanavalin-A-induced agglutination. Doses of UV which inhibit the cellular growth do not interfere with the plasma membrane. With gamma-radiation, however, doses which inhibit cellular growth also interfere with the plasma membrane. These results suggest that for certain applications UV radiation may be an advantage in vaccine production.
mesh_terms: D000371:Agglutination / Q000528:radiation effects; D000818:Animals; D001665:Binding Sites / Q000528:radiation effects; D002462:Cell Membrane / Q000378:metabolism; D003208:Concanavalin A / Q000378:metabolism; D003470:Culture Media; D056890:Eukaryota / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects*; D005720:Gamma Rays; D011839:Radiation, Ionizing; D014466:Ultraviolet Rays
chemical_list: D003470:Culture Media; D003208:Concanavalin A