[Determination of carboanhydrase activity in blood erythrocytes].
A simple method is suggested for accelerated determination of carboanhydrase activity (in 0.01 ml of blood or other biological liquid for 3.0--4.0 min). The method is based on the ability of erythrocytes carboanhydrase to induce specifically carbonic acid gas hydration accompanied by formation of carbonic acid. The latter changes the medium reaction from alkaline (pH9,0) to slightly acid (pH 6.0). The reaction end is fixed by an acid-alkaline indicator (or automatically) according to the time of the solution color changes. A simple design is developed for the device to obtain the substrate which removes the factors affecting negatively the reaction course; the optimal concentration of reagents are selected and a new indicator-buffer mixture having no inhibitatory effect on the carboanhydrase activity is used.
D002256:Carbonic Anhydrases / Q000097:blood*; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008722:Methods
D007202:Indicators and Reagents; D002256:Carbonic Anhydrases
Opredelenie karboangidraznoĭ aktivnosti v eritrotsitakh krovi.