title: [Guanylate cyclase in Escherichia coli. I. Purification of the enzyme and evidence for an inhibitor].
abstract: Guanylate cyclase activity is present in crude E. coli extract. Guanylate cyclase has been purified 3500 fold from this extract, through ammonium sulfate fractionation, DEAE-cellulose chromatography. Sephadex G-75 gel-filtration and polyacrylamide gel preparative microelectrophoresis. During the purification a guanylate cyclase inhibitor has been separated.
mesh_terms: D001426:Bacterial Proteins / Q000302:isolation & purification*; D004791:Enzyme Inhibitors / Q000302:isolation & purification; D004926:Escherichia coli / Q000201:enzymology*; D006162:Guanylate Cyclase / Q000037:antagonists & inhibitors / Q000302:isolation & purification*
chemical_list: D001426:Bacterial Proteins; D004791:Enzyme Inhibitors; D006162:Guanylate Cyclase
vernacular_title: Guanylate cyclase chez Escherichia coli. I. Purification de l'enzyme et mise en évidence d'un inhibiteur.