Identity and lipase productivity of a mesophilic actinomycete isolated from Egyptian soil.
1. A mesophilic lipolytic actinomycete was isolated from Egyptian soil and was identified as a strain of Streptomyces flavogriseus. 2. Lipase(s) produced by S. flavogriseus is (at least partly) constitutive in its (their) nature and can be produced in the absence of lipids, however, its production is stimulated in their presence. 3. S. flavogriseus was unable to grow at 40 degrees C or higher temperatures. However, lipase(s) produced at lower temperatures (e.g. 20, 25, 30 and 35 degrees C) were more active at 45 and 55 degrees C. This is probably due to the presence of a heat sensitive lipase inhibitor in the culture filtrate. 4. Optimum conditions for lipase(s) production by S. flavoriseus are pH 6.8, incubation for 48-72 hours at 35 degrees C with 0.8% castor oil as the carbon source in Dox liquid medium supplemented with 0.3% yeast extract. 5. Factors supporting good growth were not always the same as those stimulating lipase(s) production.
D003470:Culture Media; D004534:Egypt; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000096:biosynthesis*; D012988:Soil Microbiology; D013302:Streptomyces / Q000145:classification* / Q000201:enzymology / Q000502:physiology; D013696:Temperature
D003470:Culture Media; D008049:Lipase