Induction of foci of altered hepatocytes by a single injection of azaserine to rats.
The induction of foci of altered, gamma-glutamyltranspeptidase (GGT)-positive hepatocytes by azaserine was investigated. After injection of a single dose of azaserine, many foci developed in male Wistar rats fed a choline-devoid (CD) diet containing acetylaminofluorene (AAF), but only a few in rats fed a choline-supplemented (CS) diet containing AAF. Similar results were obtained in rats fed a plain CD diet or a plain CS diet and injected with a single dose of azaserine after a partial hepatectomy. These findings indicate that azaserine is an effective initiator of liver carcinogenesis in rats, and that a CD diet acts as a strong promoter of the evolution initiated liver cells to foci of altered, GGT-positive hepatocytes.
D015073:2-Acetylaminofluorene / Q000633:toxicity; D000818:Animals; D001377:Azaserine / Q000633:toxicity*; D002794:Choline / Q000494:pharmacology; D003043:Cocarcinogenesis; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced; D008297:Male; D051381:Rats; D013997:Time Factors; D005723:gamma-Glutamyltransferase / Q000378:metabolism
D001377:Azaserine; D015073:2-Acetylaminofluorene; D005723:gamma-Glutamyltransferase; D002794:Choline