Peritoneal macrophages as target cells for measuring virus-specific T cell mediated cytotoxicity in vitro.
In inbred or outbred mice lysis of virally modified cells by thymus-derived lymphocytes occurs only within a H-2 compatible system. Peritoneal macrophages provide a convenient source of syngeneic target cells. They are readily infected in vitro with LCM virus, and show high levels of specific 51Cr release when exposed to T cells from LCM immune mice.
D000818:Animals; D000956:Antigens, Viral / Q000008:administration & dosage*; D001202:Ascitic Fluid / Q000276:immunology; D003601:Cytotoxicity Tests, Immunologic; D000939:Epitopes; D007111:Immunity, Cellular; D007275:Injections, Intravenous; D008217:Lymphocytic choriomeningitis virus / Q000276:immunology; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D013601:T-Lymphocytes / Q000276:immunology*
D000956:Antigens, Viral; D000939:Epitopes