Effect of bleomycin on deoxyribonucleic acid synthesis in toluene-treated Escherichia coli cells.
The antibiotic bleomycin stimulates deoxyribonucleic acid (DNA) synthesis in toluene-treated Escherichia coli cells. The increase in synthesis is linear with bleomycin concentration. Bleomycin-stimulated DNA synthesis is independent of replication and dependent on DNA polymerase I. Replication is spared as the DNA polymerase I-dependent DNA synthesis increases. Bleomycin does not appear to have any effect on purified E. coli DNA polymerases I or II. Our results suggest that bleomycin causes nicking of the bacterial chromosome with subsequent DNA synthesis catalyzed by DNA polymerase I.
D001761:Bleomycin / Q000494:pharmacology*; D004254:DNA Nucleotidyltransferases / Q000378:metabolism; D004260:DNA Repair / Q000187:drug effects; D004269:DNA, Bacterial / Q000096:biosynthesis*; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D013997:Time Factors; D014050:Toluene / Q000494:pharmacology*
D004269:DNA, Bacterial; D001761:Bleomycin; D014050:Toluene; D004254:DNA Nucleotidyltransferases