title: [The methylene-blue-reduction-test (MR-test) and the micro-TTC-test for the determination of substances as sole source of carbon in the taxonomy of Pseudomonas species (author's transl)].
abstract: Two methods are described for the determination of substances as sole source of carbon for taxonomic tools: the Methylene-Blue-Reduction-Test and the Micro-TTC-Test. Both methods are giving results superior to the technique introduced by STANIER and co-workers and used hitherto in the taxonomic classification of Pseudomonas species. The evaluation of the comparative test results is based on the criterion that a substance is useful for taxonomic purpose provided that at least 90 per cent of the strains of a species are giving homogeneous results. - The MR-Test is superior to the Micro-TTC-Test concerning the reproducibility of the results. The Micro-TTC-Test is useful as a screening technique in the routine work and the primary investigation of numerous strains.
mesh_terms: D000081:Acetamides / Q000378:metabolism; D000333:Aeromonas / Q000378:metabolism; D001089:Arabinose / Q000378:metabolism; D001622:Betaine / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D005069:Evaluation Studies as Topic; D005947:Glucose / Q000378:metabolism; D062385:Hydroxybenzoates / Q000378:metabolism; D007202:Indicators and Reagents; D008751:Methylene Blue; D011549:Pseudomonas / Q000145:classification* / Q000378:metabolism; D013045:Species Specificity; D013194:Staining and Labeling; D013237:Stereoisomerism; D013739:Testosterone / Q000378:metabolism; D013778:Tetrazolium Salts; D014363:Tryptamines / Q000378:metabolism; D014871:Water Microbiology
chemical_list: D000081:Acetamides; D062385:Hydroxybenzoates; D007202:Indicators and Reagents; D013778:Tetrazolium Salts; D014363:Tryptamines; D001622:Betaine; D013739:Testosterone; D001089:Arabinose; D005947:Glucose; D008751:Methylene Blue
vernacular_title: Der Methylenblau-Reduktions-Test (MR-Test) und der Mikro-TTC-Test zum Nachweis der Verwertung von Substanzen für die Taxonomie der Pseudomonas-Arten im Oberflächen- und Grundwasser