Methanol precipitation of transmissible gastroenteritis virus.
Methanol precipitation of transmissible gastroenteritis virus was tested at Ph 4.0, 5.0, 6.0, and 7.0 and at methanol concentrations of 15%, 25%, and 30%. Supernatant and precipitate fractions were tested for complement-fixing and agar-diffusion soluble antigens and plaque-forming units, and were examined by electron microscopy. Virus could be obtained free of detectable agar-diffusion antigens and most of the complement-fixing antigens. Most of the virions were without peplomers after methanol treatment but they retained infectivity.
D000818:Animals; D002460:Cell Line; D003332:Coronaviridae / Q000254:growth & development*; D003470:Culture Media; D046508:Culture Techniques; D006863:Hydrogen-Ion Concentration; D008297:Male; D000432:Methanol / Q000494:pharmacology*; D011233:Precipitin Tests; D013552:Swine; D013737:Testis; D005760:Transmissible gastroenteritis virus / Q000254:growth & development*; D014776:Virus Cultivation
D003470:Culture Media; D000432:Methanol