Antigenic determinants of adenovirus capsids. I. Measurement of antibody cross-reactivity.
Evidence is presented that the type-specific antibody to the adenovirus hexon is not simply the antibody with the highest activity for cross-reactive determinants, but is a distinct, minority population that recognizes seperate determinants. To quantify it, we have developed an inhibition method with radio-immunoprecipitation (RIP) as a sensitive assay for the type-specific antibody that remains after all the excess of cross-reactive antibody has been blocked by heterologous antigen. During the primary response, 0.1 to 1% of antibody to types 2 or 5 hexon is type-specific, but after boosting, this population may reach 10 to 20%. Antibody to fiber is more than 70% type-specific during primary and secondary responses. The cross-reacting antibody can be removed on immunoabsorbent columns without affecting the virus neutralization titer of the serum.
D000256:Adenoviridae / Q000276:immunology*; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000918:Antibody Specificity; D001667:Binding, Competitive; D002213:Capsid / Q000276:immunology*; D003429:Cross Reactions; D000939:Epitopes; D006168:Guinea Pigs; D051379:Mice; D011863:Radioimmunoassay; D014764:Viral Proteins / Q000276:immunology*
D000914:Antibodies, Viral; D000939:Epitopes; D014764:Viral Proteins