[Problem in the partial separation of X- and Y-carrying human spermatozoa].
The introduction of a fluorescent staining technique for identification of Y chromosome made possible the distinction of X- and Y-bearing human spermatozoa. Using this fluorescence analysis the isolation of Y- and X-bearing human spermatozoa were investigated employing two different methods for separation of spermatozoa. 1. The migration of human spermatozoa in cervical mucus obtained from women shortly before mid-cycle was studied, using an in-vitro method for horizontal sperm penetration. Basing on the progressive sperm motility in cervical mucus, a fraction rich in Y-bearing spermatozoa in the frontal zone of sperm migration was found. 2. Gradient centrifugation at different rotor speeds using a discontinous sucrose gradient has been employed for separation of human spermatozoa. A significant trend for separation of Y- and X-bearing spermatozoa was demonstrated. The results were shortly discussed.
D002499:Centrifugation, Density Gradient / Q000379:methods; D002582:Cervix Mucus; D066298:In Vitro Techniques; D008297:Male; D008722:Methods; D012730:Sex Chromosomes / Q000032:analysis*; D013081:Sperm Motility; D013083:Sperm Transport; D013094:Spermatozoa / Q000032:analysis*; D013194:Staining and Labeling
Zur Frage der partiellen Trennung von X- und Y-tragenden Spermien beim Menschen