title: IgA in secondary anti-hapten responses in vitro.
abstract: Production of IgA anti-hapten antibodies in vitro was studied in suspension and fragment cultures of spleens from mice immunized with NIP-CG (4-hydroxy-5-iodo-3-nitrophenylacetyl coupled to chicken globulin) a few weeks earlier. IgA anti-hapten production tended to be of much shorter duration than IgG anti-hapten production. IgA produced in fragment cultures had sedimentation coefficients of 9S, 11S, and 13S. 9S IgA anti-hapten was more prominent later during the in vitro response than earlier. In suspension cultures IgM anti-hapten production preceded IgA and IgG anti-hapten production. Very little IgM anti-hapten was seen in fragment cultures, although the responses on a cell-to-cell basis tended to be much higher in fragment than in suspension cultures.
mesh_terms: D000818:Animals; D000917:Antibody Formation; D000921:Antibody-Producing Cells; D000941:Antigens; D002478:Cells, Cultured; D006241:Haptens / Q000032:analysis; D007114:Immunization; D007070:Immunoglobulin A / Q000096:biosynthesis*; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009592:Nitrohydroxyiodophenylacetate / Q000276:immunology; D013154:Spleen / Q000276:immunology; D005719:gamma-Globulins / Q000276:immunology
chemical_list: D000941:Antigens; D006241:Haptens; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D005719:gamma-Globulins; D009592:Nitrohydroxyiodophenylacetate