Slow reacting substance (SRS) from ionophore A23187-stimulated human leukemic basophils. I. Evidence for a precursor role of arachidonic acid and initial purification.
SRS was generated from human leukemic basophils upon stimulation with ionophore A23187. Radiolabel from [14C]-AA was incorporated into SRS with continued comigration of radioactivity and bioactivity through several chromatographic systems including DEAE-cellulose, silicic acid, and RP-HPLC. Human basophilic leukemia SRS displayed physiochemical properties similar to those of rat basophilic leukemia cell SRS.
D000328:Adult; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001095:Arachidonic Acids / Q000494:pharmacology*; D012898:Autacoids / Q000096:biosynthesis* / Q000302:isolation & purification; D001491:Basophils / Q000276:immunology*; D000001:Calcimycin / Q000494:pharmacology*; D002848:Chromatography, DEAE-Cellulose; D002853:Chromatography, Liquid; D004306:Dose-Response Relationship, Immunologic; D006801:Humans; D007951:Leukemia, Myeloid / Q000276:immunology*; D008297:Male; D051381:Rats; D013997:Time Factors
D000900:Anti-Bacterial Agents; D001095:Arachidonic Acids; D012898:Autacoids; D000001:Calcimycin