The effect of thiols on the immunologic release of slow reacting substance of anaphylaxis. II. Other in vitro and in vivo models.
In the presence of L-cysteine, a selective and marked enhancement of the in vitro, immunologic release of slow reacting substance of anaphylaxis (SRS-A) from human peripheral leukocytes, sensitized monkey lung fragments, and sensitized guinea pig lung fragments was observed. In the rat, cysteine, but not sodium sulfide, enhanced the calcium ionophore (A23187)- induced release of SRS-A in vitro from mixed rat peritoneal cells and in vivo from the rat peritoneal cavity. Pretreatment of rats with cysteine also enhanced the IgGa-and anti-rat IgE-mediated release of SRS-A in vivo in the rat. These studies indicate a common biochemical mechanism involved in the formation and release of SRS-A from these different tissues and cells and further confirm the observation that the rat mast cell is not a major source of SRS-A in the rat.
D000818:Animals; D001202:Ascitic Fluid / Q000276:immunology; D000001:Calcimycin / Q000494:pharmacology; D003545:Cysteine / Q000494:pharmacology*; D006168:Guinea Pigs; D000882:Haplorhini; D006636:Histamine Release; D006801:Humans; D007073:Immunoglobulin E / Q000378:metabolism; D007962:Leukocytes / Q000378:metabolism; D008168:Lung / Q000276:immunology; D008252:Macaca fascicularis; D008297:Male; D008954:Models, Biological; D051381:Rats; D013189:SRS-A / Q000378:metabolism*
D013189:SRS-A; D007073:Immunoglobulin E; D000001:Calcimycin; D003545:Cysteine