[Discontinued treatment with BudR and staining with acridine orange: observation of R- or Q- or intermediary banding (author's transl)].
Discontinued treatments with BudR at different periods of the cellular cycle produce various chromosome banding after staining with acridine orange. In particular, it is possible to observe R- or Q- or an intermediary banding, simply by varying the time of incorporation of BudR. This implies that the amount of AT or GC bases present locally in DNA is not directly responsible for the banding observed. Furthermore it appears that a precise correlation exists between replication and R- or Q-banding: the DNA located at each group of bands replicates either early (R-bands) or late (Q-bands). But these timings overlap towards the middle of phase S: if the treatment is given at that time, it is possible to observe aspects intermediary between Q and R.
D000166:Acridines; D001973:Bromodeoxyuridine; D002455:Cell Division; D002875:Chromosomes; D004261:DNA Replication; D006801:Humans; D007621:Karyotyping; D008214:Lymphocytes; D013194:Staining and Labeling; D013997:Time Factors
D000166:Acridines; D001973:Bromodeoxyuridine
Traitements discontinus par le BrdU et coloration par l'acridine orange: obtention de marquages R, Q et intermédiaires