title: Localization of a protein antigen in the chicken spleen. Effect of various manipulative procedures on the morphogenesis of the germinal centre.
abstract: The time course of the localization of a protein antigen human serum albumin (HSA) into the chicken spleen after intravenous injection is analysed. Localization within seconds to the region surrounding the Schweigger-Seidel sheaths is accomplished by HSA complexes with chicken anti-HSA or by heat aggregated HSA. The localization of soluble HSA has to await the synthesis of sufficient chicken anti-HSA to accomplish localization to the same white pulp sites in the spleen at 25-30 hours after injection. By the use of complexes of HSA-anti-HSA in ten times antigen excess, the time for localization of HSA withing germinal centres was accelerated as compared with soluble HSA, so that newly formed centres containing antigen-bearing dendritic ells were seen at 48 hours instead of 72 hours after use of soluble HSA. Neonatally bursectomized and irradiated (Bx+Irr.) birds fail to localize HSA into germinal centres or to dendritic cells within the white pulp. Heat-aggregated  human gamma-globulin (HGG) injected intravenously into Bx+Irr. birds rapidly localizes within seconds to the periphery of Schweigger-Seidel sheaths and at 24 hours can be seen attached to the surface of typical dendritic cells throughout the white pulp. Hence, heat-aggregated HGG can localize to dendritic cells in the absence of specific antibody. However, such localization to dendritic cells in Bx+ Irr. birds is not followed by segregation of the aggregated HGG-bearing dendritic cells within germinal centres--a further stage in the process which is presumed to require B cells and/or specific antibody. Localization of heat-aggregated HGG to white pulp dendritic ells was prevented by treatment with pepsin sufficient to destroy the ability of aggregated HGG to activate guinea-pig complement. Similary, in vivo decomplementation with a purified anticomplementary fraction (CoF) from the venom of Naja naja resulted in failure of intravenously injected HSA to localize to white pulp dendritic cells and failure of subsequent germinal centre formation. However, such decomplementation did not prevent the localization of aggregated HGG to white pulp dendritic cells. These facts are discussed in the light of hypotheses concerning germinal centre formation and the homeostasis of the antibody response in the bird.
mesh_terms: D000818:Animals; D000917:Antibody Formation; D000936:Antigen-Antibody Complex; D002060:Bursa of Fabricius / Q000276:immunology; D002645:Chickens; D002852:Chromatography, Ion Exchange; D003165:Complement System Proteins / Q000032:analysis; D003601:Cytotoxicity Tests, Immunologic; D003956:Dialysis; D004586:Electrophoresis; D006706:Homeostasis; D006358:Hot Temperature; D006801:Humans; D007109:Immunity / Q000528:radiation effects; D007165:Immunosuppression Therapy; D007275:Injections, Intravenous; D008297:Male; D008856:Microscopy, Fluorescence; D009024:Morphogenesis; D010434:Pepsin A / Q000494:pharmacology; D012709:Serum Albumin / Q000276:immunology*; D012910:Snake Venoms / Q000494:pharmacology; D013154:Spleen / Q000033:anatomy & histology / Q000166:cytology / Q000276:immunology*; D013997:Time Factors; D014461:Ultracentrifugation; D005719:gamma-Globulins
chemical_list: D000936:Antigen-Antibody Complex; D012709:Serum Albumin; D012910:Snake Venoms; D005719:gamma-Globulins; D003165:Complement System Proteins; D010434:Pepsin A