Non-specific cytotoxicity of wheat gliadin components towards cultured human cells.
Chromatographically separated fractions of a proteolytic digest of wheat gliadin were assayed for cytotoxic properties using cultured human embryonic intestinal, lung, kidney, adrenal, and HEp-2 cells. In all cell types noxious effects were observed microscopically over a 24 h period. The most active fraction was that previously shown to produce xylose malabsorption in subjects with coeliac disease, disruption of lysosomes, and inhibition of morphological recovery of cultured mucosa from a patient on a gluten-free diet.
D000311:Adrenal Glands; D002446:Celiac Disease / Q000473:pathology; D002460:Cell Line; D002850:Chromatography, Gel; D003208:Concanavalin A / Q000494:pharmacology; D004044:Dietary Proteins / Q000494:pharmacology; D004622:Embryo, Mammalian; D005903:Gliadin / Q000633:toxicity*; D006801:Humans; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000201:enzymology / Q000473:pathology; D007668:Kidney; D037102:Lectins / Q000494:pharmacology; D008168:Lung; D037121:Plant Lectins; D010940:Plant Proteins / Q000633:toxicity*; D014908:Triticum / Q000633:toxicity*; D014994:Xylose / Q000378:metabolism
D004044:Dietary Proteins; D037102:Lectins; D037121:Plant Lectins; D010940:Plant Proteins; D003208:Concanavalin A; D005903:Gliadin; D014994:Xylose