[Obtaining purified preparations of individual Shigella newcastle K- and O-antigens by using an ultracentrifugation method].
A modified method of obtaining the purified K- and O-antigens of Sh. newcastle with the use of ultracentrifugation is described. Serologically individual K- and O-antigens were obtained and their chemical composition was studied. Polysaccharide of the K-antigen consisted of the galacturonic acid, galactose, glucose, rhamnose xylose and hexosamine; it contained no heptoses. The O-antigen contained galactose, glucose, mannose, rhamnose, heptose and hexosamine; in difference to K-antigen was equal to 150000--160000. Thus, the K- and the O-antigen differed not only in the electrophoretic and diffuse mobility and immunological specificity, but also in the chemical composition.
D000942:Antigens, Bacterial / Q000032:analysis / Q000302:isolation & purification*; D002845:Chromatography; D002850:Chromatography, Gel; D000939:Epitopes; D007122:Immunoelectrophoresis; D007158:Immunologic Techniques; D008970:Molecular Weight; D011135:Polysaccharides, Bacterial / Q000032:analysis; D012763:Shigella flexneri / Q000276:immunology*; D013056:Spectrophotometry, Ultraviolet; D014461:Ultracentrifugation / Q000379:methods*
D000942:Antigens, Bacterial; D000939:Epitopes; D011135:Polysaccharides, Bacterial
Poluchenie ochishchennykh preparatov individual'nykh K- i O-antigneov shigell N'iukasl s ispol'zovaniem metoda ul'tratsentrifugirovaniia