Structural, functional, and idiotypic characteristics of a phosphorylcholine-binding IgA myeloma protein of C57BL/ka allotype.
An IgA phosphorylcholine (PC)-binding myeloma protein with IgCH allotypic determinants different from those of BALB/c mice is characterized. The myeloma, CBPC 2, was induced in the CB-20 strain of mice which is congenic to BALB/c but differs from it by carrying the A15 allotypic determinant of C57BL/ka mice. Sequence analysis of the CBPC 2 light chain through the first hypervariable region, as well as isoelectric point analysis, show that this chain is indistinguishable from that of T15, a PC-binding myeloma protein of BALB/c origin. The heavy chains of CBPC 2 and T15 differ by only two amino acids (positions 14 and 16) through the first hypervariable region. As measured by inhibition of precipitation, both CBPC 2 and T15 have the same specificity for PC, glycerophosphorylcholine, acetylcholine, and choline. In addition, CBPC 2 possesses the binding site-associated idiotypic determinant which is present on T15. However, like normal or induced C57BL/6 anti-PC antibody, it does not possess the nonbinding site idiotypic determinant.
D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity; D000951:Antigens, Neoplasm; D001666:Binding Sites, Antibody; D002794:Choline / Q000031:analogs & derivatives* / Q000378:metabolism; D002846:Chromatography, Affinity; D000939:Epitopes; D005997:Glycerylphosphorylcholine / Q000378:metabolism; D007070:Immunoglobulin A; D007128:Immunoglobulin Fragments; D007143:Immunoglobulin Heavy Chains; D007519:Isoantigens; D007525:Isoelectric Focusing; D007779:Lactobacillus acidophilus / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009101:Multiple Myeloma / Q000276:immunology; D009194:Myeloma Proteins / Q000302:isolation & purification; D009374:Neoplasms, Experimental / Q000276:immunology; D009943:Organophosphorus Compounds / Q000378:metabolism*; D010954:Plasmacytoma / Q000276:immunology; D011135:Polysaccharides, Bacterial; D011817:Rabbits / Q000276:immunology
D000951:Antigens, Neoplasm; D000939:Epitopes; D007070:Immunoglobulin A; D007128:Immunoglobulin Fragments; D007143:Immunoglobulin Heavy Chains; D007519:Isoantigens; D009194:Myeloma Proteins; D009943:Organophosphorus Compounds; D011135:Polysaccharides, Bacterial; D005997:Glycerylphosphorylcholine; D002794:Choline