Low molecular weight substitutes for beef extract as eluents for poliovirus adsorbed to membrane filters.
Basic solutions of beef extract and casein were able to elute poliovirus adsorbed to four membrane filters with different chemical compositions. Hydrolyzed protein and individual amino acids were able to elute virus adsorbed to certain filters but were unable to elute virus adsorbed to other filters efficiently. A solution of 4 M urea buffered at pH 9 with 0.05 M lysine was able to elute greater than 60% of the virus adsorbed to each of the filters tested. Certain solutions of amino acids were capable of eluting virus adsorbed to one filter but permitted adsorption of virus to another filter with a different chemical composition. Acidic amino acids could interfere with elution of virus from membrane filters. Aromatic compounds with an amino group attached to the ring were good eluents for virus adsorbed to epoxy-fiberglass membrane filters. In contrast, aromatic compounds with other substituents were generally poor eluents.
D000327:Adsorption; D000596:Amino Acids; D001565:Benzoates; D004492:Edetic Acid; D029822:Enterovirus B, Human / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D008239:Lysine; D008851:Micropore Filters; D008970:Molecular Weight; D017955:Poliovirus / Q000302:isolation & purification*; D011506:Proteins; D014508:Urea
D000596:Amino Acids; D001565:Benzoates; D011506:Proteins; D014508:Urea; D004492:Edetic Acid; D008239:Lysine