title: The specificity of the fluorescent antibody test using the sera of rabbits inoculated with strains of myocobacteria.
abstract: Sera from experimentally infected rabbits were used to test the specificity of the fluorescent antibody test. It was possible using mono-specific sera to differentiate antigenically Mycobacterium phlei, M fortuitum, M smegmatis, M avium, M intracellulare, M bovis (BCG) and M johnei. The cross-reactivity within the M avium and M intracellulare group was such that one antigen from these groups would detect infection within that group and exclude M johnei infection. The M phlei growth factor independent strain M johnei 316F was shown to be antigenically distinct from a M phlei dependent strain 9N96. There was loss of specificity when M avium infection was superimposed on a previous M johnei infection and when M johnei infection was superimposed on M avium infection.
mesh_terms: D000818:Animals; D003429:Cross Reactions; D000939:Epitopes; D005455:Fluorescent Antibody Technique; D009161:Mycobacterium / Q000276:immunology*; D009164:Mycobacterium Infections / Q000276:immunology / Q000662:veterinary*; D009168:Mycobacterium phlei / Q000276:immunology; D010283:Paratuberculosis / Q000276:immunology; D011817:Rabbits; D014379:Tuberculosis, Avian / Q000276:immunology
chemical_list: D000939:Epitopes