In vitro binding of various biological substances by two hypocholesterolaemic resins. Cholestyramine and colestipol.
The ability of cholestyramine and colestipol, two hypocholesterolaemic resins, to bind in vitro several compounds such as vitamin B12, vitamin B12-intrinsic factor complex, folic acid, iron citrate and calcium chloride was investigated. Both resins bound to a high extent vitamin B12-intrinsic factor complex, folic acid and iron citrate; in addition, cholestyramine also caused appreciable binding of calcium. Throughout a large range of pH, there was no change in the binding capacity; however, at pH 2, cholestyramine exhibited a marked drop in the binding of tested substances (with exception of folic acid). By increasing the molarity of the solutions, the binding to the resins of vitamin B12-intrinsic factor complex and of calcium chloride was completely inhibited. In human gastric and duodenal juices, the uptake by the resins of the studied compounds depends on the molarity of the physiological medium tested and partly confirms the results obtained with aqueous solutions. These data obtained in vitro emphasize the necessity of regular monitoring these biochemical parameters during chronic treatment of hypercholesterolaemia conducted with these two resins.
D001826:Body Fluids / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D055598:Chemical Phenomena; D002621:Chemistry; D002792:Cholestyramine Resin / Q000378:metabolism*; D003084:Colestipol / Q000378:metabolism*; D004386:Duodenum / Q000378:metabolism; D005492:Folic Acid / Q000378:metabolism; D005750:Gastric Juice / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007501:Iron / Q000378:metabolism; D011073:Polyamines / Q000378:metabolism*; D014805:Vitamin B 12 / Q000378:metabolism
D011073:Polyamines; D002792:Cholestyramine Resin; D005492:Folic Acid; D007501:Iron; D003084:Colestipol; D014805:Vitamin B 12; D002118:Calcium