Detection of structural differences between nuclear and mitochondrial glutamate dehydrogenases by the use of immunoadsorbents.
Structural differences between crystalline mitochondrial and nuclear glutamate dehydrogenases from ox liver have been detected by immunological techniques. Antisera prepared against each enzyme precipitate both glutamate dehydrogenases; upon immunodiffusion, the antiserum against the nuclear enzyme gives a line of incomplete identity with the two antigens, whereas the antiserum against the mitochondrial enzyme gives a line of complete identity. Fractionation of the antibodies contained in each antiserum by means of an immunoadsorbent, to which the nuclear or the mitochondrial enzyme has been covalently linked, shows that nuclear glutamate dehydrogenase (GDH) contains specific antigenic determinants as well as determinants common to the mitochondrial enzyme, whereas the latter appears to have no antigenic portions which are not present in the nuclear antigen, in accord with the results of immunodiffusion. The antibodies against determinants common to both enzymes precipitate and inhibit them, whereas the specific anti-nuclear GDH antibodies precipitate but do not inhibit the nuclear antigen.
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D002417:Cattle; D002467:Cell Nucleus / Q000201:enzymology*; D055598:Chemical Phenomena; D002621:Chemistry; D000939:Epitopes; D005969:Glutamate Dehydrogenase / Q000037:antagonists & inhibitors / Q000276:immunology; D008099:Liver / Q000201:enzymology*; D008930:Mitochondria, Liver / Q000201:enzymology*; D011233:Precipitin Tests; D011487:Protein Conformation
D000906:Antibodies; D000939:Epitopes; D005969:Glutamate Dehydrogenase