The membrane-bound hydrogenase of Alcaligenes eutrophus. I. Solubilization, purification, and biochemical properties.
The membrane-bound hydrogenase of Alcaligenes eutrophus was solubilized from washed membranes of autotrophically grown cells. The enzyme consists of two types of subunits and is an iron-sulfur protein. A flavin compound was not detected. The enzyme reacts only with few artificial electron acceptors.
D000421:Alcaligenes / Q000201:enzymology*; D002462:Cell Membrane / Q000201:enzymology*; D003840:Deoxycholic Acid; D006859:Hydrogen; D006863:Hydrogen-Ion Concentration; D007506:Iron-Sulfur Proteins; D008751:Methylene Blue / Q000378:metabolism; D008970:Molecular Weight; D010088:Oxidoreductases / Q000302:isolation & purification / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000494:pharmacology; D011092:Polyethylene Glycols; D012995:Solubility; D013379:Substrate Specificity
D007506:Iron-Sulfur Proteins; D010713:Phosphatidylcholines; D003840:Deoxycholic Acid; D011092:Polyethylene Glycols; D006859:Hydrogen; D010088:Oxidoreductases; D008751:Methylene Blue