[The activity and properties of fructose-1,6-diphosphatase in various tissues (Liver, Kidneys, skeletal muscle, small intestine mucosa) in swine].
The following parameters are recommended for activity determination of FDPase by release of inorganic phosphate at 37 degrees C of enzyme from liver and kidneys of swine: pH 7.5, 2 mMol concentration of fructose-1,6-diphosphate, 5 mMol concentration of Mg ions, and ten minutes of incubation. When the activity of FDPase was analysed with temperatures of 22 degrees C, and 45 degrees C, rises were recorded up to the highest temperature. ADTA was strongly activating along with 2 mMol of Mg ions in a test arrangement of 5 mMol concentration. Manganese, zinc, and cobalt ions are strong activators even in low concentrations (0.2 or 1.0 mMol), whereas copper, cadmium, and mercury ions are strong inhibitors of the enzyme. Average activities of FDPase were analysed by means of the optical test in the liver and kidneys of fetuses aged 98 days. They were 1.79 or 0.38 units in one gram of tissue. In pigs for slaughter they had been 2.06 or 3.58 units in one gram of tissue.
D000818:Animals; D004492:Edetic Acid / Q000494:pharmacology; D005333:Fetus / Q000378:metabolism; D006597:Fructose-Bisphosphatase / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007413:Intestinal Mucosa / Q000201:enzymology; D007421:Intestine, Small / Q000201:enzymology; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008274:Magnesium / Q000494:pharmacology; D009132:Muscles / Q000201:enzymology; D009928:Organ Specificity; D013552:Swine / Q000378:metabolism*; D013696:Temperature
D004492:Edetic Acid; D006597:Fructose-Bisphosphatase; D008274:Magnesium
Untersuchungen über die Aktivität und die Eigenschaften der Fruktose-1,6-diphosphatase in einigen Geweben (Leber, Niere, Skelettmuskel, Dünndarmschleimhaut) beim Schwein.