[Comparative analysis of gamma globulin preparations isolated by means of DEAE-Sephadex and DEAE-cellulose].
Preparations of rabbit gamma-globulin obtained with the aid of ion-exchange chromatography on DEAE-Sephadex contained an admixture of other serum proteins revealed by disc-electrophoresis in acrylamide gel. This impurity can be eliminated by rechromatography of gamma-globulin preparations of DEAE-cellulose in the same buffer solutions which were used for purification of gamma-globulin on DEAE-Sephadex. Better purification of gamma-globulin on DEAE-cellulose can supposedly be attributed to the effective absorption on cellulose basis of euglobulin aggregates which form in the solutions with a low ionic power used for chromatographic isolation of gamma-globulin on ion-exchangers.
D000818:Animals; D002848:Chromatography, DEAE-Cellulose; D002852:Chromatography, Ion Exchange; D011817:Rabbits; D005719:gamma-Globulins / Q000302:isolation & purification*
D005719:gamma-Globulins
Sravnitel'nyĭ analiz preparatov gamma-globulina, vydelennykh s pomoshch'iu DEAE-sefadeksa i DEAE-tselliulozy