[On the possibility to demonstrate the presence of catecholamines in enterochromaffin cells: critical review (author's transl)].
Various data contributing to support, after Lillie et al., the presence of catecholamines instead of (or in addition to) 5-hydroxytryptamine in the enterochromaffin cells, are submitted to analytical criticism by the Author. Topics of the discussion are mainly the behaviour of the diazo-reaction (in acid medium), the fluorescence induced by formaldehyde, and various reactions carried out either directly or following a chemical block. The Author demonstrates that 5-HT is present in the enterochromaffin cells and that it is impossible, at present time, to prove the presence of catecholamines in these cells on the basis of histochemical data. The Author debates also how biochemical or pharmacological tests may supply the lacking of histochemical proofs in demonstrating the presence of catecholamines; it is possible by applying these procedures to study situations like those postulated by Lillie et al., extending a comparative research also to other elements of the enterochromaffin cell system after ERSPAMER; the examples here reported however do not agree perfectly in some of the cases with these views.
D000818:Animals; D001391:Azo Compounds; D002395:Catecholamines / Q000032:analysis*; D002838:Chromaffin System / Q000166:cytology*; D004759:Enterochromaffin Cells / Q000166:cytology*; D005453:Fluorescence; D005557:Formaldehyde; D005976:Glutaral; D006651:Histocytochemistry; D007211:Indoles; D008407:Mast Cells / Q000166:cytology; D051381:Rats; D012701:Serotonin / Q000032:analysis*; D013194:Staining and Labeling
D001391:Azo Compounds; D002395:Catecholamines; D007211:Indoles; D005557:Formaldehyde; D012701:Serotonin; D005976:Glutaral
Considerazioni critiche sulla diagnosticabilità della presenza di catecolamine nelle cellule enterocromaffini