Background staining and sensitivity of the unlabelled antibody-enzyme (PAP) method. Comparison with the peroxidase labelled antibody sandwich method using formalin fixed paraffin embedded material.
The PAP procedure was compared with the peroxidase labelled antibody sandwich method and was found to be at least 20 times more sensitive. Background staining was reduced by the addition of normal swine serum to all the immune sera or by pretreating sections with it at the beginning of either method. The PAP procedure could be effectively reduced to a period of 1 hour or less.
D015100:3,3'-Diaminobenzidine; D000818:Animals; D000936:Antigen-Antibody Complex; D005455:Fluorescent Antibody Technique / Q000379:methods*; D005557:Formaldehyde; D006652:Histological Techniques; D006801:Humans; D007106:Immune Sera; D010232:Paraffin; D010544:Peroxidases / Q000276:immunology*; D013194:Staining and Labeling / Q000379:methods
D000936:Antigen-Antibody Complex; D007106:Immune Sera; D005557:Formaldehyde; D015100:3,3'-Diaminobenzidine; D010232:Paraffin; D010544:Peroxidases