Incompatibility in vitro and in vivo demonstrated only with saline-suspended red cells.
An unusual IgG complement-binding antibody was observed in a 64-year-old man prior to surgery. This antibody was detectable by the indirect antiglobulin test when the red cells were suspended in saline, but not when they were suspended in acid citrate dextrose or albumin solutions. Positive reactions were obtained with the patient's own red cells and with the cells of all donors tested. In vivo chromium survival studies showed that donor cells and patient cells, when suspended in saline, had 1-hour survivals of 32 and 46%, respectively. In contrast, donor and patient cells suspended in ACD solution had 1-hour survivals of 77 and 93%, respectively. We concluded that this phenomenon may casue accelerated destruction of saline-suspended cells and should be suspected whenever in vitro incompatibility is noted with red cells suspended in saline.
D000017:ABO Blood-Group System; D000327:Adsorption; D000906:Antibodies / Q000276:immunology; D001787:Blood Group Incompatibility / Q000276:immunology*; D003176:Complement C3 / Q000276:immunology; D003298:Coombs Test; D004905:Erythrocyte Aging; D004912:Erythrocytes / Q000276:immunology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000276:immunology; D008297:Male; D008875:Middle Aged; D012965:Sodium Chloride
D000017:ABO Blood-Group System; D000906:Antibodies; D003176:Complement C3; D007074:Immunoglobulin G; D012965:Sodium Chloride