Catalytic tritiation of drugs and analysis of the tritium distribution by 3H n.m.r. spectroscopy.
Propranolol, phenobartitone, diphenylhydantoin, amphetamine, imipramine and propoxyphene have been tritiated using tritiated water and platinum catalyst (from the dioxide and sodium borohydride), and the pattern of labelling has been ascertained by 3H n.m.r. spectroscopy. The results show that this exchange procedure can lead to satisfactory incorporation of tritium at 'stable' aromatic positions. For imipramine where incoroporation of tritium was low, an alternative acid-catalysed procedure was satisfactory.
D000661:Amphetamine / Q000032:analysis; D002384:Catalysis; D011431:Dextropropoxyphene / Q000032:analysis; D007099:Imipramine / Q000032:analysis; D007553:Isotope Labeling; D009682:Magnetic Resonance Spectroscopy; D010634:Phenobarbital / Q000032:analysis; D010672:Phenytoin / Q000032:analysis; D011433:Propranolol / Q000032:analysis; D014316:Tritium / Q000032:analysis*
D014316:Tritium; D010672:Phenytoin; D011433:Propranolol; D000661:Amphetamine; D007099:Imipramine; D011431:Dextropropoxyphene; D010634:Phenobarbital