N-demethylation of lergotrile by Streptomyces platensis.
Thirty-eight microorganisms were screened for their ability to produce metabolites of the semisynthetic alkaloid, lergotrile. A total of five microorganisms were found to biotransform lergotrile, and N-desmethyl lergotrile was detected as the principal metabolite with most organisms. Streptomyces platensis (NRRL 2364) appeared to form the metabolite in highest yield, and a preparative-scale conversion was accomplished with a recovered yield of 50%. Structure proof was accomplished with comparative thin-layer chromatography, mixed melting point, mass spectrometry, and remethylation to lergotrile.
D001711:Biotransformation; D055598:Chemical Phenomena; D002621:Chemistry; D004873:Ergolines / Q000031:analogs & derivatives / Q000096:biosynthesis / Q000378:metabolism*; D005285:Fermentation; D005658:Fungi / Q000378:metabolism; D013045:Species Specificity; D013302:Streptomyces / Q000378:metabolism*
D004873:Ergolines; C024271:N-desmethyl lergotrile