The identity of the acid and alkaline phosphatases of human seminal plasma.
Examination of human seminal plasma showed that there was no p-nitrophenylphosphatase activity maximum at alkaline pH values. A constant ratio of enzyme activities in 8 split ejaculates, identical temperature dependence and copurification through a three-step purification procedure led us to conclude that the alkaline phosphatase in human semen is identical to acid phosphatase.
D000135:Acid Phosphatase / Q000302:isolation & purification / Q000378:metabolism*; D000469:Alkaline Phosphatase / Q000302:isolation & purification / Q000378:metabolism*; D002852:Chromatography, Ion Exchange; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D012661:Semen / Q000201:enzymology*; D013696:Temperature
D000469:Alkaline Phosphatase; D000135:Acid Phosphatase