Purification of canine plasma renin by affinity chromatography.
An affinity column for the purfication of canine plasma renin was prepared using goat anti-renin (dog kidney) gammaG gloublins. The antiserum was prepared against a purified kidney renin preparation. The anti-renin globulins were coupled to cyanogen bromide activated Sepharose. Using the anti-renin globulin-coupled Sepharose as an immuno-adsorbant, a method was devised allowing purification of plasma renin to a 1,000-fold purity.
D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods; D004285:Dogs; D006041:Goats / Q000276:immunology; D005779:Immunodiffusion; D007668:Kidney / Q000276:immunology; D008970:Molecular Weight; D012083:Renin / Q000097:blood* / Q000302:isolation & purification; D005719:gamma-Globulins
D005719:gamma-Globulins; D012083:Renin