Localization of the immunoglobulins G, A and M, beta-trace protein and gamma-trace protein on isoelectric focusing of serum and cerebrospinal fluid by immunofixation.
The mobility of the immunoglobulins G, A and M, beta-trace protein and gramma-trace protein on isoleectric focusing of serum and CSF was determined by immunofixation using specific antisera. Polyclonal IgG migrated as multiple bands between pH 4.7--8.6, polyclonal IgA as multiple bands between pH 4.9--6.1 in CSF and serum. IgM could not be identified in normal CSF or serum. beta-trace protein gave three bands at pH 8.0, 8.4 and 7.4--7.5, respectively, while gamma-trace protein gave one single band at pH 9.5--greater than 9.5. Oligoclonal IgG in CSF in multiple sclerosis and neurosyphilis migrated between pH 8.6--greater than 9.5 and was easily discriminated from other proteins.
D002556:Cerebrospinal Fluid Proteins / Q000032:analysis*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007070:Immunoglobulin A / Q000032:analysis / Q000134:cerebrospinal fluid; D007074:Immunoglobulin G / Q000032:analysis / Q000134:cerebrospinal fluid; D007075:Immunoglobulin M / Q000032:analysis / Q000134:cerebrospinal fluid; D007136:Immunoglobulins / Q000032:analysis*; D007158:Immunologic Techniques; D007525:Isoelectric Focusing; D009103:Multiple Sclerosis / Q000134:cerebrospinal fluid / Q000276:immunology; D009494:Neurosyphilis / Q000134:cerebrospinal fluid / Q000276:immunology
D002556:Cerebrospinal Fluid Proteins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins