A rapid micro scale method for the detection of lysopine and nopaline dehydrogenase activities.
A rapid and sensitive method has been developed to determine lysopine dehydrogenase (EC 1.5.1-) and nopaline dehydrogenase activities in crown gall tumour tissues. By this method, enzyme activities as low as 0.2  micrometerol octopine or nopaline per h per g fresh weight tumour tissue can still be detected. In non-infected young pea seedlings, no lysopine dehydrogenase activity was detected.
D001120:Arginine / Q000031:analogs & derivatives; D003605:D-Amino-Acid Oxidase / Q000032:analysis*; D005977:Glutarates; D008239:Lysine / Q000031:analogs & derivatives; D008832:Microchemistry / Q000379:methods; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000032:analysis*; D010941:Plant Tumors / Q000201:enzymology*; D012231:Rhizobium
D005977:Glutarates; C008666:nopaline; C015333:lysopine; D001120:Arginine; D003605:D-Amino-Acid Oxidase; D000587:Oxidoreductases Acting on CH-NH Group Donors; C021882:nopaline dehydrogenase; D008239:Lysine