The isolation and characterisation of a platelet-specific beta-globulin (beta-thromboglobulin) and the detection of antiurokinase and antiplasmin released from thrombin-aggregated washed human platelets.
A protein fraction was isolated from the supernatant of thrombin-aggregated washed human platelets and was shown, by immunodiffusion techniques, to contain a platelet-specific beta-globulin (beta-thromboglobulin) as the major component. A molecular weight of 35 800 was determined for beta-thromboglobin from the measured sedimentation coefficient of3.0 S and Stokes radius of 2.85 nm. Beta-Thromboglobin was detected in the serum from whole blood and the supernatant of 48-h-old platelet-rich plasma and 28-day-old citrated whole blood, but not in platelet-poor plasma. The fraction containing beta-thromboglobulin was shown to possess an antiurokinase activity but was devoid of antiplasmin activity. A further fraction of approximate molecular weight 70 000 was also isolated which contained an antiplasmin but was devoid of antiurokinase activity.
D000818:Animals; D000933:Antifibrinolytic Agents; D001609:Beta-Globulins / Q000302:isolation & purification; D001792:Blood Platelets / Q000032:analysis* / Q000276:immunology; D002499:Centrifugation, Density Gradient; D002850:Chromatography, Gel; D010450:Endopeptidases; D005341:Fibrinolysin; D006801:Humans; D007106:Immune Sera; D005779:Immunodiffusion; D008970:Molecular Weight; D010974:Platelet Aggregation; D011817:Rabbits / Q000276:immunology; D013997:Time Factors; D014462:Ultrafiltration; D014568:Urokinase-Type Plasminogen Activator
D000933:Antifibrinolytic Agents; D001609:Beta-Globulins; D007106:Immune Sera; D010450:Endopeptidases; D005341:Fibrinolysin; D014568:Urokinase-Type Plasminogen Activator