Some biological properties of beta2-microglobulin and its antibody.
beta2-Microglobulin on the surface of lymphocytes exists in two molecular forms, first as part of the HL-A antigen complex, and second as a free molecule unbound to other membrane macromolecules. Quantitative determinations of the numbers of molecules of beta2-microglobulin per lymphocyte when compared to published values for the numbers of HL-A molecules per cell are consistent with an excess of beta2-microglobulin compared to HL-A antigens on the cell surface. Turnover studies of beta2-microglobulin from the lymphocyte surface indicated that both beta2-microglobulin and HL-A components are metabolized at similar rates. beta2-Microglobulin appears to be released in the free form. HL-A antigens, if released from the cell surface, appear to be released unbound to beta2-microglobulin. The effects of anti-beta2-microglobulin antibody on lymphocyte activation, namely on the mixed lymphocyte reaction and on antigen induced proliferative response, were studied. Anti-beta2-microglobulin completely inhibited the mixed lymphocyte reaction and the antigen induced proliferative response.
D000906:Antibodies; D000937:Antigen-Antibody Reactions; D001609:Beta-Globulins / Q000378:metabolism*; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D006680:HLA Antigens; D006649:Histocompatibility Antigens; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology* / Q000648:ultrastructure; D001613:beta 2-Microglobulin / Q000032:analysis / Q000276:immunology / Q000378:metabolism*
D000906:Antibodies; D001609:Beta-Globulins; D006680:HLA Antigens; D006649:Histocompatibility Antigens; D001613:beta 2-Microglobulin