Distinction between tumor-specific transplantation antigen and virion antigens in solubilized products from membranes of virus-induced leukemic cells.
A membrane antigen from RBL-5 leukemic cells that was solubilized and partially purified is further characterized in this study. This soluble antigen is capable of immunizating syngeneic hosts to reject neoplastic cells and thus resembles TSTA. It also induces cytotoxic antibody in syngeneic hosts capable of specifically lysing RBL-5 cells in vitro. RBL-5, however, releases infectious virus (RLV); it was necessary therefore to rule out virus or structural virion antigens as the effective immunogen. Infectious virus was not detectable in our initial crude membrane (CM) material, nor in the papain-solubilized CS or the G-150 Sephadex-chromatographed fraction. Virus-neutralizing antibody was not detected, under stringent assay conditions, in the syngeneic anti-CM sera. Antigen preparations CM, CS and the chromatographeal fractions F1, F2 and F3 were assayed in a complement-fixation test against brood-reacting antisera capable of detecting virus envelope antigen and gs antigen and against syngeneic antisera. Although our antigen preparations were positive for virion antigens, CS and F2 contained an antigen that reacted only with syngeneic antiserum. These same fractions were those reactive as immunogens. On the basis of these data, it is postulated that a cellular membrane component, other than viral, functions as TSTA.
D000818:Animals; D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D002462:Cell Membrane / Q000276:immunology; D003168:Complement Fixation Tests; D003601:Cytotoxicity Tests, Immunologic; D000939:Epitopes; D006649:Histocompatibility Antigens; D007106:Immune Sera; D009052:Leukemia Virus, Murine / Q000276:immunology; D007942:Leukemia, Experimental / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009500:Neutralization Tests; D012190:Retroviridae / Q000276:immunology
D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D000939:Epitopes; D006649:Histocompatibility Antigens; D007106:Immune Sera