Histochemical staining in lymphocyte suspensions complexed with beta-galactosidase as an antigen. Unspecific staining of spleen cells by indigo.
Spleen cells from mice immunized with E. coli beta-galactosidase were incubated with the enzyme and with 5-bromo-4-chloroindolyl-beta-galactosidase as a histochemical substrate. Some cells are stainable in this reaction, however, not all of them carry specific receptors for beta-galactosidases. Some spleen cells can take up dye and thereby become unspecifically stained. This system is therefore not suitable for the detection of antigen binding to cells.
D000818:Animals; D001666:Binding Sites, Antibody; D005696:Galactosidases / Q000276:immunology*; D008214:Lymphocytes / Q000032:analysis* / Q000276:immunology; D051379:Mice; D013154:Spleen / Q000166:cytology*; D013194:Staining and Labeling
D005696:Galactosidases