Sequential Q- and Acridine orange-marker technique.
A standardized Q- and acridine orange (AO)-fluorescence dual marker technique was described. It involved preservation of unstained chromosome slides in a vacuum desiccator up to 18 months, Q-staining, destaining, and treatment in Hanks' solution, pH 5.1, at 85 degrees C for 13 min, and acridine orange staining. Q-markers were found at the paracentromeric regions of chromosomes 3 and 4, the short arms and the satellites of the acrocentric chromosomes, while AO-marker spots were on the satellite-stalks of the acrocentrics. The advantage of the dual marker technique was illustrated by the determination of the origin of trisomy 22 in a spontaneous abortus.
D000022:Abortion, Spontaneous / Q000235:genetics; D000166:Acridines; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000032:analysis*; D002901:Chromosomes, Human, 13-15; D002904:Chromosomes, Human, 21-22 and Y; D005260:Female; D006801:Humans; D008297:Male; D011247:Pregnancy; D011797:Quinacrine Mustard; D013194:Staining and Labeling; D014314:Trisomy
D000166:Acridines; D011797:Quinacrine Mustard