A rotating bottle culture method with continuous replacement of the gas phase.
A 'rotator' culture method is described which provides a continuous flow of oxygenating gas to cultures in rotating bottles. The system maintains constant O2 and CO2 levels in the culture medium throughout the incubation period. It also provides a more stable pH than systems with sealed culture bottles.
D000818:Animals; D002245:Carbon Dioxide; D002478:Cells, Cultured; D046508:Culture Techniques / Q000295:instrumentation*; D004477:Ectogenesis; D006863:Hydrogen-Ion Concentration; D010100:Oxygen
D002245:Carbon Dioxide; D010100:Oxygen