Correlation between in vivo and in vitro functional tests for activated macrophages.
Mice undergoing graft-versus-host reaction, skin grafting, and inoculation with tumor cells were tested for nonspecific resistance by intravenous challenge with Listeria monocytogenes. Peritoneal exudate macrophages from mice treated in a similar manner were tested in vitro for increased degradation of [1-14C]glucose, ability to degrade antigen/antibody complexes, ability to inhibit intracellular growth of listeria, and staining for beta-galactosidase. There was good correlation between in vivo resistance towards L. monocytogenes and in vitro inhibition of intracellular growth. There was also good correlation between increase in beta-galactosidase and in vivo resistance in mice undergoing a graft-versus-host-reaction.
D000818:Animals; D000936:Antigen-Antibody Complex; D005260:Female; D005947:Glucose / Q000378:metabolism*; D006087:Graft vs Host Reaction; D008089:Listeria monocytogenes / Q000254:growth & development*; D008264:Macrophages / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D009368:Neoplasm Transplantation; D013154:Spleen / Q000382:microbiology; D014184:Transplantation, Homologous; D001616:beta-Galactosidase / Q000378:metabolism
D000936:Antigen-Antibody Complex; D001616:beta-Galactosidase; D005947:Glucose