Standardization of the alkaline elution procedure using X-ray-damaged nuclear DNA.
Structural modification of DNA were induced by X-irradiation of crude hepatic nuclei at various dose ranges to standardize DNA damage evaluated by the alkaline elution technique. This quantitative assay can be used as reference for DNA damage induced by the in vivo administration of mutagens and/or carcinogens involved in the environment.
D000468:Alkalies; D000818:Animals; D002467:Cell Nucleus / Q000528:radiation effects; D004247:DNA / Q000528:radiation effects*; D004277:DNA, Single-Stranded / Q000302:isolation & purification*; D004307:Dose-Response Relationship, Radiation; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D008297:Male; D008722:Methods; D051379:Mice; D008807:Mice, Inbred BALB C; D012015:Reference Standards; D014965:X-Rays
D000468:Alkalies; D004277:DNA, Single-Stranded; D004247:DNA