Anti-immunoglobulin stimulation of murine lymphocytes. I. Age dependency of the proliferative response.
The in vitro proliferative response of normal mouse spleen cells to anti-immunoglobulin (Anti-Ig) reagents was found to be an age-associated phenomenon. The response usually appears in mice when they reach 5 to 7 months of age and is rarely seen in younger animals. Anti-Ig induced proliferation was observed by using two different antisera--one polyvalent, prepared against mouse antibody-antigen complexes and one prepared against mouse IgM myeloma. Both antisera were shown to be specific for B cells by cytotoxicity and immunofluorescent staining. Glassbead separation of spleen cells showed that the anti-Ig-induced proliferation was a B cell response.
D000375:Aging; D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000276:immunology; D007106:Immune Sera / Q000494:pharmacology; D007075:Immunoglobulin M; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D013154:Spleen / Q000276:immunology; D005719:gamma-Globulins
D000888:Antibodies, Anti-Idiotypic; D007106:Immune Sera; D007075:Immunoglobulin M; D005719:gamma-Globulins