title: Accumulation of N,N-dimethyltryptamine in rat brain cortical slices.
abstract: 14C-DMT accumulates in rat brain cortical slices incubated at 37 C. This process has many of the properties of an active uptake mechanism. It is temperature-sensitive, sodium-dependent, saturable, and is inhibited by metabolic inhibitors. Tryptamine derivatives were more effective than the catecholamines in competing for 14C-DMT accumulation. A number of psychotropic drugs were inhibitors of 14C-DMT accumulation. In general, irrespective of pharmacologic class, the tertiary amines were more potent than the secondary or primary amines, although there were some exceptions. Most of the accumulated 14C-DMT was associated with the cytoplasmic fraction. Of the portion associated with the crude mitochondrial fraction, 54.4% was associated with nerve-ending fraction.
mesh_terms: D000818:Animals; D014150:Antipsychotic Agents / Q000494:pharmacology; D002395:Catecholamines / Q000494:pharmacology; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D046508:Culture Techniques; D008297:Male; D004130:N,N-Dimethyltryptamine / Q000037:antagonists & inhibitors / Q000378:metabolism*; D009994:Osmolar Concentration; D051381:Rats; D013347:Subcellular Fractions / Q000378:metabolism; D013696:Temperature; D014363:Tryptamines / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D014150:Antipsychotic Agents; D002395:Catecholamines; D014363:Tryptamines; D004130:N,N-Dimethyltryptamine