[Study of the reproduction conditions of the avian myeloblastosis virus for separation of the "reverse transcriptase" enzyme].
Sensitivity of eight chick lines to the avian myeloblastosis virus as the main source for RNA-dependent DNA-polymerase recovery was studied in the course of the "revertase" project. The virus (0.1 ml) was inoculated intracardially or intraperitoneally to one-day chicks, and then the virus titer was determined according to the ATP-activity. C and D-lines of the Enya-cross were shown to be most sensitive (sensitivity 75%) among the lines studied.
D000818:Animals; D001354:Avian Leukosis Virus / Q000201:enzymology*; D009189:Avian Myeloblastosis Virus / Q000201:enzymology* / Q000254:growth & development; D002645:Chickens; D012194:RNA-Directed DNA Polymerase / Q000302:isolation & purification*; D014776:Virus Cultivation
D012194:RNA-Directed DNA Polymerase
Izuchenie uslovii razmnozheniia virusa ptich'ego mieloblastoza dlia vydeleniia fermenta "obratnaia transkriptaza"