Antigen-antibody interactions and the anomalous kinetics of arylsulfatase A.
Antibodies against homogeneous rabbit liver arylsulfatase A (aryl-sulfatase sulfohydrolase, EC 3.1.6.1) were produced in a goat and the effects of these antibodies on the kinetic parameters of the enzyme have been studied. The results indicate that the binding of antibody to the enzyme does not alter the enzyme active site, since Km and -ki values are unaffected. However, a small reduction in the enzyme activity was observed as the result of a reduction of V in the enzyme-antibody complex. The binding of antibodies led to a change in the pH-rate profile, giving one broad pH optimum shifted toward higher pH value. The enzyme-antibody complex still showed the characteristic arylsulfatase A anomalous kinetics at pH 5.5, but the inactivation was significantly slower than for the native enzyme. As calculated from quantitative immuno-precipitation data, the native enzyme bound 5--7 molecules of IgG. The number of IgG molecules which bound to the turnover-modified enzyme was reduced to 3--4. The loss of antigenic determinants from the turnover-modified enzyme indicates that significant conformational changes occur during the turnover-induced modification, or that a covalent modification of residues present at the antigenic sites has occurred, or both.
D000818:Animals; D000937:Antigen-Antibody Reactions; D001192:Arylsulfatases / Q000276:immunology; D002553:Cerebroside-Sulfatase / Q000378:metabolism*; D006041:Goats / Q000276:immunology; D006863:Hydrogen-Ion Concentration; D007106:Immune Sera; D005779:Immunodiffusion; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D011817:Rabbits; D013429:Sulfatases / Q000378:metabolism*
D007106:Immune Sera; D013429:Sulfatases; D001192:Arylsulfatases; D002553:Cerebroside-Sulfatase