C- and G-bands of the opossum chromosomes: terminal sequences of DNA replication.
The sex chromosomes of the opossum, Didelphys virginiana, are the only elements that exhibit C-banding. In contrast, the sex chromosomes as well as the autosomes bear specific G-Bands. However, unlike other mammalian species different types of G-banding are observed if the chromosomes are pretreated with trypsin and SSC solution The SSC-pretreated chromosomes show discrete bands only when stained with Giemsa at certain pH values. An asynchronous pattern of terminal DNA replication is observed among the three C-banding regions of the X-chromosome. The inter- and intrapositive G-banding areas of the chromosomes are not always late in DNA replication in comparison to those negatively stained G-banding areas.
D000818:Animals; D001399:Azure Stains; D002875:Chromosomes / Q000378:metabolism; D004261:DNA Replication; D006863:Hydrogen-Ion Concentration; D007621:Karyotyping; D008297:Male; D008938:Mitosis; D009893:Opossums / Q000033:anatomy & histology*; D012730:Sex Chromosomes; D013194:Staining and Labeling
D001399:Azure Stains