Analysis of herpes simplex virus isolated from patients with recurrent herpes keratitis exhibiting "treatment-resistance" to 5-iodo-2'-deoxyuridine.
Four strains of herpes simplex virus (HSV) were isolated from two patients with recurrent herpes keratitis who failed to respond to 5'-iodo-2'-deoxyuridine (IDU) treatment. Two of the four isolates were highly resistant to IDU in cell culture and the other two isolates were more susceptible to IDU than an HSV-1 laboratory strain. From each patient, an IDU-resistant and an IDU-susceptible virus was isolated. All 4 isolates possessed the ability to induce the thymidine kinase (TK) activity in cell lines lacking that activity. All the isolates were type 1 HSV, since the filamentous structures, recognized as a biological marker of type 2 HSV, were not observed in infected cells.
D000818:Animals; D002460:Cell Line; D004352:Drug Resistance, Microbial; D004790:Enzyme Induction; D006801:Humans; D007065:Idoxuridine / Q000494:pharmacology* / Q000627:therapeutic use; D007635:Keratitis, Dendritic / Q000188:drug therapy / Q000382:microbiology*; D051379:Mice; D012008:Recurrence; D018139:Simplexvirus / Q000187:drug effects* / Q000254:growth & development; D013937:Thymidine Kinase / Q000096:biosynthesis
D013937:Thymidine Kinase; D007065:Idoxuridine