Immobilization of microbial cells containing NAD-kinase.
Microbial cells having NAD-kinase activity, Brevibacterium ammoniagenes, were immobilized by the radiation-copolymerization method under low temperature with the activity recovery of more than 80%. Compared to the native microbial cells the immobilized cells were more stable against heat and pH change. The immobilized cells were subjected to the 5 hr reaction repeatedly 20 times without any activity loss.
D001951:Brevibacterium / Q000201:enzymology* / Q000528:radiation effects; D004800:Enzymes, Immobilized / Q000378:metabolism / Q000528:radiation effects; D005720:Gamma Rays; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009243:NAD / Q000378:metabolism; D010770:Phosphotransferases / Q000378:metabolism / Q000528:radiation effects; D011108:Polymers; D013696:Temperature
D004800:Enzymes, Immobilized; D011108:Polymers; D009243:NAD; D010770:Phosphotransferases