Ir-associated murine alloantigens. Serological and chemical definition of Ia specificities associated with the H-2-b haplotype.
Strains bearing recombinant H-2 haplotypes with known positions of recombination have been used to demonstrate antibodies reacting with Ia antigens associated with the H-2-b haplotype in several H-2 antisera. Two new Ia specificities, Ia.8 and Ia.9, both determined by genes in the Ir-1A subregion, have thus been defined, and the strain distribution of these specificities has been determined. Immunoprecipitation of radio-labeled membrane antigens has been used to characterize Ia.9 and to distinguish it chemically from Ia.7 in a membrane preparation from a strain possessing both specificities.
D000818:Animals; D000937:Antigen-Antibody Reactions; D011232:Chemical Precipitation; D002860:Chromium Radioisotopes; D002874:Chromosome Mapping; D003434:Crossing Over, Genetic; D003601:Cytotoxicity Tests, Immunologic; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes; D006238:Haploidy; D007106:Immune Sera / Q000302:isolation & purification; D007118:Immunoassay; D007120:Immunochemistry; D007125:Immunogenetics; D007519:Isoantigens; D051379:Mice; D008815:Mice, Inbred Strains; D008970:Molecular Weight; D011995:Recombination, Genetic
D002860:Chromium Radioisotopes; D000939:Epitopes; D007106:Immune Sera; D007519:Isoantigens