The role of H-2 and Ia antigens in graft-versus-host reactions (GVHR). Presence of host alloantigens on donor cells after GVHR and suppression of GVHR with an anti-Ia antiserum against hose Ia antigens.
By using an indirect immunofluorescence technique, the presence of host cell derived H-2K, H-2D, and Ia alloantigens on donor cells recovered from recipient spleens after a graft-versus-host response (GVHR) was demonstrated. Mapping studies indicated that only host K, D, and I-A region gene products could be identified on the donor cells. Host I-E/C- and I-J-subregion products were not absorbed by donor cells. Treatment of activated donor cells with anti-Ly sera plus C' revealed that donor cells carrying host Ia antigens have a Ly-1+,2-,3- phenotype, whereas donor cells carrying H-2K and H-2D host antigens have a Ly-1-,2+,3+ phenotype. A GVHR that resulted from only an I-region incompatibility was suppressed by the injection of recipient mice with an anti-Ia antiserum directed against self Ia antigens. The degree of suppression was proportional to the amount of anti-Ia antiserum administered.
D000818:Animals; D000954:Antigens, Surface; D006087:Graft vs Host Reaction; D006183:H-2 Antigens / Q000235:genetics; D007165:Immunosuppression Therapy; D007518:Isoantibodies; D007519:Isoantigens / Q000235:genetics; D008285:Major Histocompatibility Complex; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology*
D000954:Antigens, Surface; D006183:H-2 Antigens; D007518:Isoantibodies; D007519:Isoantigens