[Genetic control of the content and avidity of antigen-binding B-lymphocytes in the mouse spleen].
The content (per 10 nucleated cells) and avidity curves of IgM positive B-lymphocytes forming rosettes with trinitrophenyl-sheep erythrocytes (TNP-RFC) were compared in two mouse lines (BALB/c C57BL/6), F1 hybrids (BALB /c X C57BL/6) and backcross hybrids (F1 X BALB/c, F1 X C57BL/6). Trinitrophenyl-bovine serum albumin (TNP24BSA) and dinitrophenyl-bonvine serum albumin (DNP23BSA) and sulfanil-BSA (Sulf17-BSA) in different dilutions were used as inhibitors. BALB/c spleen contained by 60% more TNP-RFC than C576/6 spleen. F1 hybrids (F1 X BALB/c) contained on the average by 35% more TNP-RFC than (F1 X C57BL/6). Inhibition curves (avidity curves) were essentially different in the two mouse lines and F1 hybrids. A conclusion was drawn that the TNP-REC content and avidity were under a strong genetic control. Together with the assumption of random expression of V-genes (idiotype genes?) in the lymphocytes the above data suggest that the simplest mechanism of the genetic control could be a definitive ratio between the corresponding groups of V-genes.
D000818:Animals; D000941:Antigens; D001402:B-Lymphocytes / Q000276:immunology*; D001666:Binding Sites, Antibody; D000939:Epitopes; D006824:Hybridization, Genetic; D007125:Immunogenetics; D007075:Immunoglobulin M / Q000032:analysis; D007166:Immunosuppressive Agents / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D012397:Rosette Formation; D013154:Spleen / Q000166:cytology* / Q000276:immunology
D000941:Antigens; D000939:Epitopes; D007075:Immunoglobulin M; D007166:Immunosuppressive Agents
Geneticheskiĭ kontrol' soderzhaniia i avidnosti antigensviazyvaiushchikh B-limfotsitov v selezenke myshi.