T-T interactions in the induction of antigen-specific human suppressor T lymphocytes in vitro.
We intended to investigate whether the suppression of antigen-induced antibody responses in vitro in man by T suppressor cells required contact of T suppressor cells with target cells or whether this effect was mediated by factors released by T suppressor cells. To this end supernatants of antigen-induced T suppressor cells were tested (by a plaque forming cell assay) for their capacity to suppress antibody responses of autologous and allogeneic human peripheral blood lymphocytes. We have shown that supernatants of antigen-specific T suppressor cells, designated as TsF24: a) can suppress an antibody response of autologous but not allogeneic lymphocytes to the inducing antigen; b) are antigen-specific in their effect; and 3) are produced by radiosensitive T cells. Furthermore, the target of the factor is a radiosensitive T cell. These findings taken together indicate that, in the generation of T-effector suppressor cells in man, T-T interactions occur, and in addition, that cellfree factors may be involved in these interactions.
D000818:Animals; D002450:Cell Communication; D003513:Cycloheximide / Q000494:pharmacology; D004306:Dose-Response Relationship, Immunologic; D000939:Epitopes; D004912:Erythrocytes / Q000276:immunology; D005720:Gamma Rays; D006462:Hemolytic Plaque Technique; D006358:Hot Temperature; D006801:Humans; D008285:Major Histocompatibility Complex; D012756:Sheep; D013601:T-Lymphocytes / Q000276:immunology; D014357:Trypsin / Q000494:pharmacology
D000939:Epitopes; D003513:Cycloheximide; D014357:Trypsin