Estimation of the sizes of polymorphic C-bands in man by measurement of DNA content of whole chromosomes.
A method is proposed for estimating the sizes of polymorphic C-bands of human chromosomes by microdensitometric measurement of the DNA content of whole chromosomes. The method requires measurements of: a chromosome known not to by polymorphic, as a standard against which to normalize all measurements; the polymorphic chromosomes of interest; and a homologous polymorphic chromosome lacking the C-band. In an example studies here, the C-band of chromosome 9 was estimated to contain about 0.0296 pg of DNA.
D002877:Chromosomes, Human / Q000032:analysis*; D002906:Chromosomes, Human, 6-12 and X / Q000032:analysis; D004247:DNA / Q000032:analysis*; D005821:Genetic Techniques; D006801:Humans; D011110:Polymorphism, Genetic; D013194:Staining and Labeling
D004247:DNA