Tumor-specific and tumor-associated membrane antigens of Rous sarcoma virus transformed hamster fibroblasts.
Hamster fibroblasts transformed by an env- strain of Rous sarcoma virus (RSV) express at their surface tumor-associated antigens of unknown origin and a tumor-specific antigen (VCSA) which is not expressed by hamster fibroblasts transformed by unrelated DNA or RNA oncogenic viruses. This antigen was detectable by rabbit antibodies and a complement-dependent 51Cr-release cytotoxicity assay and is common to RSV-transformed cells of different animal species. By comparing the anti-VCSA serum which antisera directed against purified gp85, gs-proteins, reverse transcriptase or detergentlysed virus particles, it was shown that VCSA is not a known virion structural protein. Moreover, VCSA expression does not correlate with viral replication since it is not detectable in chick embryo fibroblasts productively infected with the transformation-defective virus RAV-1 which shares virus structural genes with RSV. Finally, in hamster cells transformed by an RSV mutant, temperature-sensitive for the ability to transform the host cell, VCSA expression at the cell surface correlates with the expression of the transforming gene.
D000818:Animals; D000914:Antibodies, Viral; D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D001358:Avian Sarcoma Viruses / Q000276:immunology*; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology*; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D002642:Chick Embryo; D003165:Complement System Proteins; D006224:Cricetinae; D003601:Cytotoxicity Tests, Immunologic; D004279:DNA, Viral; D000939:Epitopes; D005347:Fibroblasts / Q000276:immunology; D005814:Genes, Viral; D007106:Immune Sera; D012367:RNA, Viral; D011817:Rabbits; D051381:Rats; D014779:Virus Replication
D000914:Antibodies, Viral; D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D004279:DNA, Viral; D000939:Epitopes; D007106:Immune Sera; D012367:RNA, Viral; D003165:Complement System Proteins