Demonstration of neurosecretory substance in previously scanned specimens: adaptation of the Gomori aldehyde-fuchsin method for correlative SEM/TEM/LM histochemistry.
The Gomori aldehyde-fuchsin (AF) method for selective staining of neurosecretory substance (NSS) has been adapted to tissue previously prepared for both scanning and transmission electron microscopy (SEM/TEM). The procedure results in precise correlation of light microscopic (LM) histochemistry with SEM/TEM of the same tissue.
D000818:Animals; D000882:Haplorhini; D006651:Histocytochemistry; D008251:Macaca; D008854:Microscopy, Electron / Q000379:methods*; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D009489:Neurosecretion; D010902:Pituitary Gland / Q000378:metabolism* / Q000648:ultrastructure; D013194:Staining and Labeling