Combined stain for fish pituitary.
A new combined stain is described for the study of cell types in the fish pituitary. Tissues are prepared by fixing in formol-sublimate and then embedded in paraffin wax. Tissue is sectioned at 5 micron and than stained sequentially with performic acid-alcian blue, periodic acid-Schiff, orange G, and acid fuchsin. As a result of this procedure acidophils stain as follows: lactotropes, red; corticotropes, light pink; melanotropes, bright pink; and somatotropes, orange. Cyanophils stain either magenta red (gonadotropes) or blue (thyrotropes). Neurosecretory material and the fibers of the pars nervosa which penetrate the pars intermedia stain light blue.
D000423:Alcian Blue; D000818:Animals; D001391:Azo Compounds; D005399:Fishes / Q000033:anatomy & histology*; D005561:Formates; D009282:Naphthalenesulfonates; D010503:Periodic Acid-Schiff Reaction; D010545:Peroxides; D010902:Pituitary Gland / Q000166:cytology*; D012394:Rosaniline Dyes; D013194:Staining and Labeling / Q000379:methods*
D001391:Azo Compounds; D005561:Formates; D009282:Naphthalenesulfonates; D010545:Peroxides; D012394:Rosaniline Dyes; C008817:peroxyformic acid; C008710:Orange G; D000423:Alcian Blue