Sedimentation velocity separation: a preparation method for cervical samples.
A preparation procedure, aiming at monolayer deposition of cervical exfoliative material on glass slides for high resolution prescreening has been developed. The main features of this procedure are centrifugal deposition after suspension and sedimentation of samples over isopycnic medium of 1.026 density. Fractioning of the separation column after centrifugation at 50 X g yields two preparations with leukocytes, bacteria and cellular debris predominantly located on the first slide and epithelial cells on the second one. The degree of spatial cellular isolation as well as the amount of diagnostically relevant cells per slide seem to fit the requirements of automated high resolution analysis.
D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient / Q000295:instrumentation; D004847:Epithelial Cells; D005260:Female; D006801:Humans; D013194:Staining and Labeling; D014626:Vaginal Smears