Immunological analysis of A strain mice bearing the A-10 mammary adenocarcinoma.
The present investigation was undertaken to determine whether antitumor antibodies are produced by A strain mice during the growth of a transplantable mammary adenocarcinoma (A-10). The antibody response was monitored by a sensitive radioimmunoassay which can detect 1 ng of antibody. No evidence of a humoral antitumor response was observed in animals given i.p. or s.c. injections of A-10 ascites cells. Control experiments showed that a humoral response was detectable 1 week after the inoculation of an allogeneic tumor. Immunoglobulin binds nonspecifically to cells via an Fc portion of the immunoglobulin molecule, and this was seen with a tumor bearer serum pool and with immunoglobulin preparations eluted from A-10 ascites cells. No specific antitumor antibody was found in these sources. The A strain mice could not be immunized to reject a challenge of live A-10 cells with mitomycin C-treated A-10 cells, with neuraminidase-treated A-10 cells, or with A-10 membrane preparations. It was concluded that the A-10 tumor is not immunogenic in its host of origin.
D000230:Adenocarcinoma / Q000276:immunology*; D000818:Animals; D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm / Q000008:administration & dosage; D000939:Epitopes; D005260:Female; D006085:Graft Survival; D007136:Immunoglobulins; D008325:Mammary Neoplasms, Experimental / Q000276:immunology*; D051379:Mice; D008805:Mice, Inbred A / Q000276:immunology*; D009368:Neoplasm Transplantation; D014185:Transplantation, Isogeneic
D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D000939:Epitopes; D007136:Immunoglobulins