Hormone-sensitive lipase of adipose tissue.
Some physiologic aspects of the mobilization and fate of free fatty acids are reviewed. The molecular mechanism of the activation of hormone-sensitive lipase in adipose tissue is then discussed. Recent evidence established that hormone-sensitive lipase, concerned with fat mobilization, is both functionally and immunochemically distinct from lipoprotein lipase, concerned with uptake of plasma triglycerides. Lipoprotein lipase activity is not altered by cyclic AMP-dependent protein kinase. The latter enzyme enhances not only triglyceride hydrolase but also monoglyceride, diglyceride and cholesterol ester hydrolase activities in chicken adipose tissue. Finally, it is shown that the activation of all four acyl hydrolases is reversible, the deactivation being magnesium-dependent. Protein phosphatase fractions from heart and liver active against phosphorylase a can reversibly deactivate adipose tissue hormone-sensitive lipase, implying a low degree of substrate specificity for lipase phosphatase.
D000273:Adipose Tissue / Q000201:enzymology* / Q000378:metabolism; D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism; D004789:Enzyme Activation; D000939:Epitopes; D005230:Fatty Acids, Nonesterified / Q000097:blood / Q000378:metabolism; D006728:Hormones / Q000494:pharmacology*; D006801:Humans; D007328:Insulin / Q000494:pharmacology; D008071:Lipoprotein Lipase / Q000276:immunology* / Q000378:metabolism; D008079:Lipoproteins, VLDL / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008994:Monoacylglycerol Lipases / Q000378:metabolism; D010750:Phosphoproteins; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism; D002787:Sterol Esterase / Q000276:immunology / Q000378:metabolism*
D000939:Epitopes; D005230:Fatty Acids, Nonesterified; D006728:Hormones; D007328:Insulin; D008079:Lipoproteins, VLDL; D010750:Phosphoproteins; D011956:Receptors, Cell Surface; D000242:Cyclic AMP; D011494:Protein Kinases; D002787:Sterol Esterase; D008994:Monoacylglycerol Lipases; D008071:Lipoprotein Lipase; D010744:Phosphoric Monoester Hydrolases