Preparing and staining of Coxiella burnetii natural phase II antigen for the microagglutination reaction.
An improved method of preparing Coxiella burnetii natural phase II antigen of high purity enabled its staining for use in the microagglutination reaction (MAR). The antigen was as specific and sensitive for detection of phase II antibodies as the artificial phase II C. burnetii antigen prepared by periodate treatment from purified phase I cells of C. burnetii.
D000372:Agglutination Tests; D000907:Antibodies, Bacterial / Q000032:analysis; D000942:Antigens, Bacterial / Q000302:isolation & purification; D003381:Coxiella / Q000166:cytology / Q000276:immunology*; D006801:Humans; D011778:Q Fever / Q000175:diagnosis; D013194:Staining and Labeling; D014357:Trypsin
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D014357:Trypsin