Detection of a nuclear, EBNA-type antigen in apparently EBNA-negative Herpesvirus papio (HVP)-transformed lymphoid lines by the acid-fixed nuclear binding technique.
In agreement with the findings of previous authors, we could not detect a virally determined nuclear antigen in Herpesvirus papio (HVP)-transformed baboon lymphoid lines by anticomplementary staining in situ, as for EBNA. However, by means of our recently developed acid-fixed nuclear binding technique an EBNA-like antigen could be readily demonstrated, after extraction from both producer and non-producer lines. We propose to designate the antigen as HUPNA. It can be detected by a human anti-EBNA antibody, suggesting cross-reactivity, if not identity, between EBNA and HUPNA. HVP-DNA carrying non-producer lines, negative for in situ ACIF stainability but capable of yielding HUPNA by the nuclear binding technique, can be superinfected with EBV, with brilliant EBNA expression as the result, suggesting that the defective in situ staining is a property associated with the baboon HVP, rather than the baboon lymphoid cell per se.
D000085:Acetates; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D002460:Cell Line; D002472:Cell Transformation, Viral; D000939:Epitopes; D000882:Haplorhini; D006564:Herpesviridae; D004854:Herpesvirus 4, Human / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology; D008722:Methods; D010215:Papio; D013194:Staining and Labeling
D000085:Acetates; D000956:Antigens, Viral; D000939:Epitopes