The synthesis of a variant-specific antigen by Trypanosoma brucei in vitro.
A variant-specific surface antigen from a cloned population of Trypanosoma brucei S42 has been isolated and partially characterized. [35S]L-methionine was found to be incorporated into this material by cells incubated in vitro in a chemically defined medium. Incorporation of [35S]L-methionine was inhibited by cycloheximide and puromycin at concentrations which are known to specifically inhibit protein synthesis in other systems. The rate of synthesis of the variant-specific antigen in vitro has been estimated to be about 8% of the rate in vivo. Newly synthesized [35S]L-methionine-labelled variant-specific antigen was incorporated into the surface coat.
D000818:Animals; D000941:Antigens / Q000302:isolation & purification; D003470:Culture Media; D000939:Epitopes; D006023:Glycoproteins / Q000096:biosynthesis / Q000302:isolation & purification; D008715:Methionine / Q000378:metabolism; D014346:Trypanosoma brucei brucei / Q000276:immunology* / Q000378:metabolism
D000941:Antigens; D003470:Culture Media; D000939:Epitopes; D006023:Glycoproteins; D008715:Methionine