Effects of various drugs on the osmotic lysis of rat mast cells.
1. Rat mast cells were exposed to low osmotic pressures to produce a 'non-specific' disruption of the mast cell, with release of histamine along with other intracellular contents. 2. The effect of non-steroidal anti-inflammaotry drugs and various other drugs upon osmotically induced histamine release was examined. 3. Representative non-steroidal anti-inflammatory drugs indomethacin, phenylbutazone and flufenamic acid, the acidic compounds ethacrynic acid, iopanoic acid and probenecid, and the local anaesthetic lignocaine, all caused a dose-dependent facilitation of osmotically induced histamine release compared to controls. All drugs were active at 0.1 mmol/l. 4. The previously observed inhibition of compound 48/80 and the antigen-induced histamine release from rat mast cells by similar concentrations of the drugs used in the present study are unlikely to be due to mast cell plasma membrane stabilization.
D000818:Animals; D004976:Ethacrynic Acid / Q000494:pharmacology; D006636:Histamine Release / Q000187:drug effects*; D007213:Indomethacin / Q000494:pharmacology; D007480:Iopanoic Acid / Q000494:pharmacology; D008297:Male; D008407:Mast Cells / Q000276:immunology*; D009997:Osmotic Pressure; D010653:Phenylbutazone / Q000494:pharmacology; D011339:Probenecid / Q000494:pharmacology; D051381:Rats
D007480:Iopanoic Acid; D010653:Phenylbutazone; D004976:Ethacrynic Acid; D011339:Probenecid; D007213:Indomethacin