[Increase of the concentration of effector T-lymphocytes specific to antigens H2. 1. Elution of T-lymphocytes from the allogeneic target cells].
The technique of elution by pronase of murine lymph node cells adherent to relevant allogeneic target cells has been developed for enrichment of effectory T cell population. A fraction was obtained of killer cells possessing cytotoxic activity which exceeds that of the initial immune lymphocyte population by a factor of 6--8 as judged by the number of lymphocytes required for a 50% cytotoxic effect. The gain in CE is fairly reproducible in different H-2 systems and under various lymphocyte-target incubation conditions.
D000818:Animals; D000951:Antigens, Neoplasm; D001666:Binding Sites, Antibody; D003601:Cytotoxicity Tests, Immunologic; D000939:Epitopes; D008198:Lymph Nodes / Q000276:immunology; D051379:Mice; D009368:Neoplasm Transplantation; D011402:Pronase / Q000494:pharmacology; D012513:Sarcoma, Experimental / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014184:Transplantation, Homologous
D000951:Antigens, Neoplasm; D000939:Epitopes; D011402:Pronase
Uvelichenie kontsentratsii effektornykh T-limfotsitov, spetsifichnykh k antigenam N-2. I. Eliutsiia T-limfotsitov s allogennykh kletok-misheneĭ.