[Accelerated production of permanent preparations of whole plant embryo sacs and pollen complexes].
Original methods are described for producing constant preparations of plant integral embryo sacs and pollen complexes isolated by means of tissue maceration with Helix pomatia gastric juice enzymes. The Feulgen stained preparations are put into the Canada balsam. According to this procedure there is no need of paraffin treatment and work with a microtome; it makes use of other methods which highly accelerate the production of constant preparations. In connection with putting the object in Canada balsam the method of treatment and the succession of operations are changed as compared to the routine procedure of producing total preparations of embryo sacks.
D006652:Histological Techniques; D010944:Plants / Q000196:embryology*; D011058:Pollen; D013194:Staining and Labeling / Q000379:methods
Uskorennoe izgotovlenie postoiannykh preparatov tselykh zarodyshevykh meshkov i pyl'tsevykh kompleksov rasteniĭ.