Detection of herpes-simplex viral genome in brain tissue.
Herpes-simplex virus type (HSV-1) nucleic-acid sequences were detected by in-situ hybridisation in thin sections of brains from mice which had been inoculated 24 weeks previously with HSV-1. These mice were not ill, and infectious virus could not be recovered from them. HSV-1 sequences were also present in brain smears from 3 of 4 elderly patients who had died with chronic psychiatric illness and neuropathological changes but not in smears from 2 patients who had had acute psychotic episodes and minimum abnormal histology. Adenovirus type 7 nucleic-acid sequences were not detected in these human specimens.
D000260:Adenoviruses, Human / Q000235:genetics; D000368:Aged; D000818:Animals; D001345:Autoradiography; D001921:Brain / Q000382:microbiology*; D004279:DNA, Viral / Q000302:isolation & purification*; D004671:Encephalitis, Arbovirus / Q000382:microbiology; D005260:Female; D005455:Fluorescent Antibody Technique; D005814:Genes, Viral; D006801:Humans; D007553:Isotope Labeling; D008297:Male; D051379:Mice; D008854:Microscopy, Electron; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D018139:Simplexvirus / Q000235:genetics* / Q000302:isolation & purification; D014316:Tritium
D004279:DNA, Viral; D014316:Tritium