Chicken egg yolk stabilizes the reverse transcriptase activity in type C particles produced by cultured MOPC-315 murine myeloma cells.
Type C particles released from cultured murine myeloma MOPC-315 cells were significantly protected when the purification steps were all conducted in the presence of 10% chicken egg yolk fluid. The yolk fluid also slowed down the inactivation of viral particles during incubation at 37 degrees C and enabled full recovery of viral particles through several cycles of freezing and thawing. The purification of viral particles in the presence of yolk fluid did not affect the capability of the viral DNA polymerase to reverse-transcribe the virion RNA in vitro, nor that of the viral RNA to act as a functional template.
D000818:Animals; D002460:Cell Line; D002500:Centrifugation, Isopycnic; D002642:Chick Embryo; D004527:Egg Proteins / Q000494:pharmacology*; D004530:Egg Yolk; D005260:Female; D005615:Freezing; D006358:Hot Temperature; D051379:Mice; D008807:Mice, Inbred BALB C; D010954:Plasmacytoma; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*; D012190:Retroviridae / Q000187:drug effects* / Q000201:enzymology
D004527:Egg Proteins; D012194:RNA-Directed DNA Polymerase