Differential staining and segregation of parental chromosomes in mouse-rabbit hybridomas.
Hoechst 33258 fluorescent staining can be coupled with G-banding to identify the chromosomal contribution of each parent in mouse-rabbit hybridomas. A fast and essentially complete segregation of rabbit chromosomes is obtained in these cells. The rabbit X chromosome is preferentially maintained in media imposing HGPRT activity for cell growth. Mouse-rabbit hybridomas, some of which secrete rabbit immunoglobulin chains, should be a convenient material for the identification of chromosomes governing rabbit Ig chain synthesis.
D000818:Animals; D001562:Benzimidazoles; D006690:Bisbenzimidazole; D002871:Chromosome Banding; D002874:Chromosome Mapping; D002875:Chromosomes / Q000032:analysis*; D005260:Female; D006822:Hybrid Cells / Q000648:ultrastructure*; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics; D007136:Immunoglobulins / Q000096:biosynthesis*; D051379:Mice; D009101:Multiple Myeloma; D011817:Rabbits; D014960:X Chromosome
D001562:Benzimidazoles; D007136:Immunoglobulins; D007041:Hypoxanthine Phosphoribosyltransferase; D006690:Bisbenzimidazole