Determination of the bleomycin-inactivating enzyme in biopsies.
A method for the determination of the Bleomycin-inactivating enzyme is described. The amount of conversion of active Bleomycin to inactive Bleomycin is used as a measure for the determination of the Bleomycin-inactivating enzyme activity. The active Bleomycin is determined in a DNA-dependent DNA polymerase assay; in a certain Bleomycin concentration range the resulting reduction of the activity of the DNA-dependent DNA polymerase is correlated linearly when plotted semilogarithmically. By application of this method it has been found that the activity of the Bleomycin-inactivating enzyme is highest in liver and present in lower concentrations in testis, spleen, lung, brain, and skin.
D001761:Bleomycin / Q000032:analysis / Q000037:antagonists & inhibitors / Q000378:metabolism*; D001921:Brain / Q000201:enzymology; D004259:DNA-Directed DNA Polymerase / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D007931:Leucyl Aminopeptidase / Q000032:analysis; D008099:Liver / Q000201:enzymology; D008168:Lung / Q000201:enzymology; D008297:Male; D012867:Skin / Q000201:enzymology; D013154:Spleen / Q000201:enzymology; D013737:Testis / Q000201:enzymology
D001761:Bleomycin; D004259:DNA-Directed DNA Polymerase; D007931:Leucyl Aminopeptidase