Translation of mRNA for glutamate dehydrogenase and spectrophotometric procedure to follow the enzyme biosynthesis.
Heterogeneous poly (A)-mRNA fraction was isolated from rat liver microsomes using phenol-chloroform extraction, millipore filtration and poly (U)-agarose affinity chromatography. Obtained fractions were characterized with respect to their secondary structure and poly (A) content. Isolated poly (A)-mRNA fraction contained high template activity for glutamate dehydrogenase in cell-free systems with microsomes or polysomes. A spectrophotometric procedure to follow enzyme biosynthesis was also developed.
D000818:Animals; D005969:Glutamate Dehydrogenase / Q000096:biosynthesis*; D008099:Liver / Q000378:metabolism; D008862:Microsomes, Liver / Q000378:metabolism*; D009690:Nucleic Acid Conformation; D011061:Poly A / Q000378:metabolism; D011132:Polyribosomes / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D012333:RNA, Messenger; D011061:Poly A; D005969:Glutamate Dehydrogenase