Properties of H-2L locus products in allogeneic and H-2 restricted, trinitrophenyl-specific cytotoxic responses.
The role of the recently defined L antigen (a second D region product) in allogeneic and TNP-specific syngeneic primary CML responses has been investigated. The lysis by anti-L specific cytotoxic effector cells was not inhibited when the target cells were pretreated with an antiserum directed against K and D, whereas an antiserum against L completely abrogated this response. Therefore, H-2L products are recognized on the target cell independently of H-2K and H-2D locus products. Both A.SW cells as well as B10 cells were found to respond to Ld alloantigens, in addition to Dd alloantigens when stimulated by cells differing only in the D region. The results of cold target blocking and antiserum inhibition experiments failed to detect cytotoxic cells with specificity of L antigens in association with TNP, under conditions in which TNP-specific effectors to K and D antigens were demonstrable. These findings suggest that there is a more limited involvement of H-2L locus products than the H-2K or H-2D  locus products in the induction and specificity of these responses.
D000818:Animals; D001667:Binding, Competitive; D002874:Chromosome Mapping; D003602:Cytotoxicity, Immunologic; D000939:Epitopes; D006649:Histocompatibility Antigens / Q000235:genetics*; D007106:Immune Sera / Q000494:pharmacology; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D009578:Nitrobenzenes / Q000276:immunology*; D014176:Protein Biosynthesis; D013154:Spleen / Q000276:immunology; D014301:Trinitrobenzenes / Q000276:immunology*
D000939:Epitopes; D006649:Histocompatibility Antigens; D007106:Immune Sera; D009578:Nitrobenzenes; D014301:Trinitrobenzenes