Hemolytic plaque formation to allogeneic human type A and B red cells.
Plaque-forming cells can be generated from peripheral blood lymphocytes provided that the lymphocytes are prestimulated by in vitro sensitization in the presence of pokeweed mitogen. Of 91 blood type O persons whose lymphocytes were stimulated with red cell A antigen, 52% responded with plaque formation, whereas of 40 type O persons whose lymphocytes were stimulated with red cell B antigen, only 20% produced plaques. Similarly, reciprocal stimulation of type A and B persons showed higher plaque formation (65%) when 23 type B persons were stimulated with A than when 48 type A persons were stimulated with B red cells (19%). An optimal dilution of sensitizing cells is necessary and maximum time of sensitization was approximately 7 days.
D000939:Epitopes; D004912:Erythrocytes / Q000276:immunology*; D006462:Hemolytic Plaque Technique; D006801:Humans; D007518:Isoantibodies / Q000096:biosynthesis; D007519:Isoantigens; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D011043:Pokeweed Mitogens / Q000494:pharmacology
D000939:Epitopes; D007518:Isoantibodies; D007519:Isoantigens; D011043:Pokeweed Mitogens