Effects of porcine plasmin on the coagulation and fibrinolytic systems in humans.
Pig plasmin (Lysofibrin) was given to 11 patients with phlebographically verified venous thrombosis, 2 of whom were treated two and three times, respectively. The effect on coagulation and fibrinolytic parameters was studied. The platelet count, Owren's P&P (prothrombin plus factors VII and X), plasminogen, factor XIII, and antithrombin III did not change during the treatment. All patients developed a proteolytic activity demonstrable on both unheated and heated fibrin plates. The fibrinogen decreased successively to very low levels, and parallel to this an increase in fibrin/fibrinogen degradation products was found. The factor VIII and factor V activities decreased immediately after each Lysofibrin infusion but normalized rapidly again. The factor VIII molecule, however, retained its reactivity to rabbit antiserum against factor VIII. Immediately after the plasmin infusion a decrease of both alpha2-macroglobulin (alpha2-M) and the rapidly reacting alpha2-antiplasmin was observed. alpha2-M decreased successively and in several of the patients values were unmeasurable for a period of some days. A complex formation between pig plasmin and the alpha2-antiplasmin was demonstrated in crossed immunoelectrophoresis. The complexes were rapidly cleared from the circulation. No interaction between the pig plasmin and the inhibitor of the plasminogen activation, alpha1-antitrypsin or inter-alpha-inhibitor, was found.
D000328:Adult; D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects*; D001780:Blood Coagulation Tests; D005169:Factor VIII / Q000378:metabolism; D005341:Fibrinolysin / Q000276:immunology / Q000627:therapeutic use*; D005342:Fibrinolysis / Q000187:drug effects*; D006801:Humans; D007106:Immune Sera; D007123:Immunoelectrophoresis, Two-Dimensional; D008297:Male; D010447:Peptide Hydrolases / Q000378:metabolism; D013552:Swine; D013924:Thrombophlebitis / Q000188:drug therapy; D000511:alpha-Macroglobulins / Q000378:metabolism
D007106:Immune Sera; D000511:alpha-Macroglobulins; D005169:Factor VIII; D010447:Peptide Hydrolases; D005341:Fibrinolysin