Cellular basis of tolerance to serum albumin in adult mice. I. characterization of T suppressor and T helper cells.
The surface markers and size of suppressor cells were determined in adult (BALB/c x C57BL/Ka)F1 mice which were tolerized with a single injection of deaggregated bovine serum albumin (BSA). Suppressor cells from the spleens of tolerazided donors were assayed in a cell transfer system in which graded numbers of cells were injected into irradiated syngeneic mice along with limiting numbers of T cells primed to BSA and an excess of B cells primed to DNP-BSA. Adoptive hosts were challenged with DNP-BSA in saline, and the anti-DNP response was measured. Suppressor cells were antigen specific as shown by the inhibitory activity of BSA-tolerant spleen cells on the response to DNP-BSA, but not to DNP-BGG. Suppressor cells were eliminated by in vitro treatment with anti-Thy 1.2, anti-Ly-2.2, anti-I-J subregion antisera and C, but not with anti-Ly-1 or anti-I-A subregion antisera. Neither unprimed nor primed helper T cells were detected in the spleen of tolerized donors after in vitro treatment with anti-Ly-2.2 antisera. Both helper and suppressor T cells from the spleens of primed or tolerized donors, respectively, showed a rapid sedimentation velocity (S greater than 3.7 mm/hr).
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D004140:Dinitrophenols; D005260:Female; D007108:Immune Tolerance; D008211:Lymphocyte Cooperation; D051379:Mice; D011828:Radiation Chimera; D012710:Serum Albumin, Bovine; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology* / Q000637:transplantation; D005719:gamma-Globulins
D004140:Dinitrophenols; D005719:gamma-Globulins; D012710:Serum Albumin, Bovine