title: Modified tannin-osmium conductive staining method for non-coated scanning electron microscope specimens. Its application to microdissection scanning electron microscopy of the spleen.
abstract: Supplement of guanidine hydrochloride to the original tannic acid solution of MURAKAMI (1973) eliminated, without debilitating the conductivity effect, the vexatious treatment with different amino-acids and sucrose in the previous method (MURAKAMI, 1974). The procedures and advantages of this simplified modification are described using human spleen samples; attempt was made to elucidate the structure of arterial terminals by dissecting the specimens under the scanning electron microscope.
mesh_terms: D004210:Dissection / Q000379:methods; D006801:Humans; D047348:Hydrolyzable Tannins; D008855:Microscopy, Electron, Scanning; D013154:Spleen / Q000648:ultrastructure*; D013194:Staining and Labeling
chemical_list: D047348:Hydrolyzable Tannins