[Modified method of differential staining of sister chromatids].
A modified method of obtaining differential staining of sister chromatids is described. It is simple, rapid, and effective, and at the same time inexpensive and accessible, since it allows one to use available reagents. When 5-bromdeoxyuridine was administered 24 hours before fixation into the Chinese hamster cell culture the percentage of metaphases with a differential chromatid staining constituted 95--98, and when this substance was administered 28 hours before fixation into the human lymphocyte culture this percentage varied from 75 to 92, depending on the individual. The mean number of sister chromatid exchanges in human lymphocytes failed to depend on the time of fixation.
D000818:Animals; D001973:Bromodeoxyuridine; D002478:Cells, Cultured; D002842:Chromatids / Q000648:ultrastructure*; D002999:Clone Cells; D006224:Cricetinae; D006801:Humans; D008214:Lymphocytes / Q000648:ultrastructure*; D013194:Staining and Labeling / Q000379:methods
D001973:Bromodeoxyuridine
Modifitsirovannyĭ metod differentsial'noĭ okraski sestrinskikh khromatid.