Ultrastructural localization of acetylcholinesterase in cultured cells. II. Cycloheximide-treated embryo muscle.
The acetylcholinesterase activity (AChE) of cultured chick embryo muscle fibers that remains after the cells have been treated with the protein synthesis inhibitor cycloheximide was examined with cytochemical stains and the electron microscope. AChE activity that decreased rapidly after addition of the inhibitor was associated with enzyme within the cells, and AChE activity that was relatively insensitive to the inhibitor was associated with AChE outside of the cells. The results support the view that there are at least two fractions of AChE in developing muscle fibers, one intracellular and labile, the other extracelullar and stable.
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D002478:Cells, Cultured; D002642:Chick Embryo; D003513:Cycloheximide / Q000494:pharmacology*; D006651:Histocytochemistry; D007527:Isoenzymes / Q000378:metabolism; D008566:Membranes / Q000201:enzymology; D009132:Muscles / Q000201:enzymology* / Q000648:ultrastructure; D013194:Staining and Labeling
D007527:Isoenzymes; D003513:Cycloheximide; D000110:Acetylcholinesterase