[A ruthenium red-osmium tetroxide section staining method (author's transl)].
We suggest a method for the section staining of polyanions by means of ruthenium red and osmium tetroxide. A 5 min incubation in diluted ruthenium red-OsO4 solution pH = 7.4 of ultrathin Durcupan-sections previously treated with sodium methoxide results in the opaque stining of mast cell granules, cartilage proteoglycans, and various epithelial mucosubstances.
D000818:Animals; D006025:Glycosaminoglycans / Q000032:analysis*; D006651:Histocytochemistry; D007422:Intestines / Q000032:analysis; D008407:Mast Cells / Q000032:analysis / Q000648:ultrastructure; D051379:Mice; D009992:Osmium; D009993:Osmium Tetroxide; D011509:Proteoglycans / Q000032:analysis; D011817:Rabbits; D051381:Rats; D012428:Ruthenium; D012430:Ruthenium Red; D013194:Staining and Labeling
D006025:Glycosaminoglycans; D011509:Proteoglycans; D012430:Ruthenium Red; D009992:Osmium; D012428:Ruthenium; D009993:Osmium Tetroxide
Rutheniumrot-OsO4-Kontrastierung am Ultradünnschnitt.