[Microvilli membrane proteins from dog enterocytes].
Mcrovilli membranes have been isolated from dog jejunal and ileal enterocytes. Proteins were analysed by polyacrylamide gel electrophoresis after solubilization with sodium dodecylsulphate. The recovery of the membrane fraction with this purification method was found to be 22% and the specific activity of sucrase increases 19 folds in the membrane fraction. Microvilli membrane proteins after polyacrylamide gel electrophoresis were seprated in 21 bands, most of them with a molecular weight higher than 70 000. Seven bands with molecular weight from 150 000 to more than 340 000, were found to be glycoproteins.
D000818:Animals; D004285:Dogs; D006023:Glycoproteins / Q000032:analysis; D007082:Ileum / Q000032:analysis*; D007413:Intestinal Mucosa / Q000032:analysis*; D007583:Jejunum / Q000032:analysis*; D008565:Membrane Proteins / Q000032:analysis* / Q000302:isolation & purification; D008970:Molecular Weight
D006023:Glycoproteins; D008565:Membrane Proteins
Etude des protéines de la bordure en brosse des entérocytes du chien.