RNA-reverse transcriptase complex from cultured human myeloma-leukemia cells.
A high molecular weight RNA-reverse transcriptase complex in the culture media of peripheral leukocytes obtained from two Japanese patients with myeloma-leukemia was detected by demonstration of a 3H-uridine peak and a peak of DNA polymerizing activity banding at a density of 1.15-1.19g/ml. The enzyme in the complex was able to utilize poly(rA)-d(pT)10 or poly (rC)-d(pG) 12-18, but not poly (dA)-d(pT) 10 or (dT) 12-18 as template-primers. The sucrose density sedimentation analysis revealed that RNA in the complex sedimented at a location of approximately 50s and 20-30s.
D000328:Adult; D002478:Cells, Cultured; D003470:Culture Media; D005260:Female; D006801:Humans; D007938:Leukemia / Q000201:enzymology*; D007962:Leukocytes / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D009101:Multiple Myeloma / Q000201:enzymology*; D012313:RNA / Q000378:metabolism*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*
D003470:Culture Media; D012313:RNA; D012194:RNA-Directed DNA Polymerase