title: Immunological cross reactivity of four enzymes involved in the biosynthetic pathway of lysine, methionine and threonine in Escherichia coli K12.
abstract: In Escherichia coli K12 the biosynthetic pathway of lysine, methionine and threonine is characterized by three isofunctional aspartokinases and two homoserine dehydrogenases. A single polypeptide chain carries the threonine-sensitive aspartokinase and homoserine dehydrogenase (AK I-HDH I), and a different polypeptide chain carries the methionine-repressible aspartokinase and homoserine dehydrogenase (AK II-HDH II). Immuno-adsorbants prepared with rabbit antibodies against AK I-HDH I bind the lysine-sensitive aspartokinase (AK III), the AK II-HDH II, and the homoserine kinase (HSK), an enzyme of the threonine biosynthetic pathway. Saturation of the immunoadsorbant with AK I-HDH I results in a decreased binding capacity for the other enzymes. Displacement of bound AK III or HSK can be obtained with pure AK I-HDH I, showing that the affinity of the antibodies to homologous antigens is higher than to heterologous ones. Immunoadsorbants prepared with anti-HSK antibodies show the same type of recognition: binding of the three aspartkinases and a capacity to displace the heterologous antigens bound. Accordingly, the same antibodies, implicated in the binding of the homologous antigen, bind the other enzymes. None of the other enzymes of the pathway, or the other kinases tested are recognized by the two immunoadsorbants. It can be postulated that in E. coli K12, duplication of a common ancestor gene gave rise to the three aspartokinases and to the homoserine kinase; two of the genes coding for the aspartokinases fused with those coding for the homoserine dehydrogenases. Indicating that only few epitopes are shared by these enzymes, by conventional immuno-diffusion techniques no precipitation lines appeared with antibodies against AK I-HDH I and the other proteins.
mesh_terms: D000429:Alcohol Oxidoreductases / Q000276:immunology*; D001222:Aspartate Kinase / Q000276:immunology*; D001426:Bacterial Proteins; D005075:Biological Evolution; D003429:Cross Reactions; D000939:Epitopes; D004926:Escherichia coli / Q000201:enzymology* / Q000378:metabolism; D006715:Homoserine Dehydrogenase / Q000276:immunology*; D007527:Isoenzymes / Q000276:immunology*; D008239:Lysine / Q000096:biosynthesis; D008715:Methionine / Q000096:biosynthesis; D010770:Phosphotransferases / Q000276:immunology*; D013912:Threonine / Q000096:biosynthesis
chemical_list: D001426:Bacterial Proteins; D000939:Epitopes; D007527:Isoenzymes; D013912:Threonine; D008715:Methionine; D000429:Alcohol Oxidoreductases; D006715:Homoserine Dehydrogenase; D010770:Phosphotransferases; D001222:Aspartate Kinase; D008239:Lysine