title: Tumor-associated antigens of chemically-induced murine tumors; the emergence of MuLV and fetal antigens after serial passage in culture.
abstract: Using radioiodinated Staphylococcus aureus protein A [125I]SPA to measure syngeneic, allogeneic and heterogeneic IgG bound to murine tumor cells, we performed a serological analysis of surface antigens of 8 solid tumors and 2 leukemias of BALB/c mice (3 chemically-induced colon carcinomas, 3 chemically-induced sarcomas, 1 murine leukemia virus (MuLV) induced leukemia, 1 irradiation induced leukemia, 1 spontaneous melanoma and 1 spontaneous sarcoma). We were able to detect and distinguish between at least five separate antigenic specificities on these tumors. Unique tumor-associated antigens were found on 3 of the tumors, MuLV related antigens on 8 tumors, fetal antigens on 7 tumors and two distinct common antigens on 7 tumors (common antigen 1 (CA-1) on 5 tumors and common antigen 2 (CA-2) on 2 tumors). Neither of the common antigens was found to be sarcoma, carcinoma or tissue-tupe specific. A number of tumors which did not originally express either MuLV or fetal antigens in primary cultures expressed these antigens after several serial passages in vitro.
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D000956:Antigens, Viral; D002272:Carcinoembryonic Antigen; D002277:Carcinoma / Q000276:immunology; D003429:Cross Reactions; D046508:Culture Techniques; D000939:Epitopes; D005260:Female; D007114:Immunization; D007074:Immunoglobulin G; D009052:Leukemia Virus, Murine / Q000276:immunology*; D007942:Leukemia, Experimental / Q000276:immunology; D008545:Melanoma / Q000276:immunology; D051379:Mice; D009374:Neoplasms, Experimental / Q000139:chemically induced / Q000276:immunology*; D012513:Sarcoma, Experimental / Q000276:immunology; D013205:Staphylococcal Protein A / Q000276:immunology
chemical_list: D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D000956:Antigens, Viral; D002272:Carcinoembryonic Antigen; D000939:Epitopes; D007074:Immunoglobulin G; D013205:Staphylococcal Protein A