Evidence for a gonad-specific receptor for H-Y antigen: binding of exogenous H-Y antigen to gonadal cells is independent of beta 2-microglobulin.
This report addresses the question whether two different types of binding exist for the reaction of H-Y antigen with the cell surface. Anti-H-Y antiserum in the presence of complement was cytotoxic only for gonadal cells expressing their own H-Y antigen, but not to ovarian cells loaded with H-Y antigen. H-Y antigen was co-redistributed with beta 2--microglobulin on newborn testicular cells, but some residual H-Y activity was found on similarly treated testis cells from 15 day old rats. After beta 2--microglobulin redistribution, testis cells maintained their binding capacity for exogenous H-Y antigen prepared from epididymal fluid or Daudi cell culture supernatants. This result suggests that exogenous H-Y antigen is bound via a gonad-specific receptor which is independent of beta 2--microglobulin and that this type of binding for H-Y antigen is different from the beta 2--m-associated expression of H-Y antigen on the cell surface.
D000818:Animals; D001609:Beta-Globulins / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D003601:Cytotoxicity Tests, Immunologic; D005260:Female; D006182:H-Y Antigen; D007152:Immunologic Capping; D008297:Male; D008560:Membrane Fluidity; D008565:Membrane Proteins / Q000378:metabolism; D010053:Ovary / Q000378:metabolism*; D051381:Rats; D011955:Receptors, Drug / Q000378:metabolism*; D013737:Testis / Q000378:metabolism* / Q000648:ultrastructure; D001613:beta 2-Microglobulin / Q000378:metabolism*
D001609:Beta-Globulins; D006182:H-Y Antigen; D008565:Membrane Proteins; D011955:Receptors, Drug; D001613:beta 2-Microglobulin