Histochemical evidence for sulfomucins at the surface of Hela cells.
The nature of the negatively charged groups present at the surface of HeLa cells was further investigated. Therefore we applied a series of light microscopic staining techniques, widely used for the demonstration of epithelial mucosubstances on tissue sections, to HeLa cells from suspension cultures. Our histochemical findings confirmed the presence of carboxylated substances at the surface of these cells. Furthermore we obtained conclusive evidence for the presence of sulfated molecules. Both substances seem to be closely related to epithelial sialomucins and sulfomucins.
D000423:Alcian Blue; D000447:Aldehydes; D002462:Cell Membrane / Q000032:analysis*; D003959:Diamines; D006025:Glycosaminoglycans / Q000032:analysis*; D006367:HeLa Cells / Q000032:analysis; D006801:Humans; D007501:Iron; D010504:Periodic Acid; D012394:Rosaniline Dyes; D013194:Staining and Labeling
D000447:Aldehydes; D003959:Diamines; D006025:Glycosaminoglycans; D012394:Rosaniline Dyes; D010504:Periodic Acid; D007501:Iron; D000423:Alcian Blue