3'-terminal nucleotide sequence of encephalomyocarditis virus RNA determined by reverse transcriptase and chain-terminating inhibitors.
We have adapted the chain-termination method for determining the nucleotide sequence of DNA of Sanger, Nicklen, and Coulson [(1977) Proc. Natl. Acad. Sci. USA 74, 5463--5467] for use with reverse transcriptase (RNA-directed DNA nucleotidyltransferase) on RNA templates. With this method and using a primer (the octanucleotide pdT7rC) directed at the 3'-terminal poly(A), we have determined a sequence of 166 residues in the genomic RNA of the picornavirus encephalomyocarditis virus.
D001483:Base Sequence; D003854:Deoxyribonucleotides / Q000494:pharmacology; D004686:Encephalomyocarditis virus / Q000235:genetics*; D008722:Methods; D012367:RNA, Viral / Q000235:genetics*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D018894:Reverse Transcriptase Inhibitors; D013698:Templates, Genetic
D003854:Deoxyribonucleotides; D012367:RNA, Viral; D018894:Reverse Transcriptase Inhibitors; D012194:RNA-Directed DNA Polymerase