[The effect of inosine, inorganic phosphates and pyruvate on erythrocyte glycolysis metabolites under conditions of preservation].
Human erythrocytes were stored as resuspensions in solutions containing citrate (Z), inosine + citrate (I), inosine + phosphate (IP), and inosine + phosphate + pyruvate (IPP). The storage was made at + 4 degrees C for 6 weeks; the initial pH-value amounted to 7.4 at + 4 degrees C. The cellular concentrations of 2.3 DPG, ATP, G6P, FDP and DOAP + GAP were determined. The following results were obtained: 1. During the storage in stored Z-blood the 2.3 DPG concentration will fall below 10% of its initial value; it will remain nearly unchanged in stored I-blood and will increase to 170% in stored IP-blood, to 270% of its initial value in stored IPP-blood. 2. The ATP concentration of cells will fall to about 50% of its initial value at the beginning of the storage of all stored blood. After that it will only increase to about 80% of its initial value in stored IP- and IPP-blood. 3. During the storage the G6P concentration will increase to the highest degree in stored IPP-blood and if high pyruvate concentrations are not present, it will have a reciprocal behaviour towards the FDP and triosephosphate level. The results were discussed in view of the regulation of glycolysis under storage conditions.
D000255:Adenosine Triphosphate / Q000097:blood; D001793:Blood Preservation; D004099:Dihydroxyacetone Phosphate / Q000097:blood; D004163:Diphosphoglyceric Acids / Q000097:blood; D004912:Erythrocytes / Q000187:drug effects*; D005636:Fructosephosphates / Q000097:blood; D005958:Glucosephosphates / Q000097:blood; D005994:Glycerophosphates / Q000097:blood; D006019:Glycolysis / Q000187:drug effects; D006801:Humans; D007288:Inosine / Q000494:pharmacology*; D010710:Phosphates / Q000494:pharmacology*; D011773:Pyruvates / Q000494:pharmacology*
D004163:Diphosphoglyceric Acids; D005636:Fructosephosphates; D005958:Glucosephosphates; D005994:Glycerophosphates; D010710:Phosphates; D011773:Pyruvates; D004099:Dihydroxyacetone Phosphate; D007288:Inosine; D000255:Adenosine Triphosphate
Der Einfluss von Inosin, anorganischem Phosphat und Pyruvat auf die Glykolysematabolite in Erythrozyten unter Konservierungsbedingungen