Pre-treatment of serum samples for immunonephelometric analysis by precipitation with polyethylene glycol.
A pre-treatment schedule for plasma samples to be analysed with immunonephelometry has been worked out using polyethylene glycol 6000 for precipitating the lipoproteins. The pre-treatment conditions have been studied in detail. By decreasing the blanks in immunonephelometric analysis a greater sensitivity of the method was gained. this was shown by performing analyses of S-beta2-microglobulin to concentrations below 1 mg/l. Another advantage with such a pretreatment is that blank determinations can be omitted for S-transferrin analysis and probably other analyses since the blanks in different samples are reduced to almost the same level.
D001609:Beta-Globulins / Q000032:analysis*; D001774:Blood Chemical Analysis / Q000379:methods*; D002498:Centrifugation / Q000379:methods; D011232:Chemical Precipitation; D002784:Cholesterol / Q000097:blood; D005470:Fluorometry / Q000379:methods; D006801:Humans; D007158:Immunologic Techniques; D008027:Light; D011092:Polyethylene Glycols; D012542:Scattering, Radiation; D013997:Time Factors; D014168:Transferrin / Q000032:analysis; D014280:Triglycerides / Q000097:blood; D001613:beta 2-Microglobulin / Q000032:analysis*
D001609:Beta-Globulins; D014168:Transferrin; D014280:Triglycerides; D001613:beta 2-Microglobulin; D011092:Polyethylene Glycols; D002784:Cholesterol