Karyotypic analyses of parental and hybrid intraspecific mouse cells, A9/B82, by giemsa- and centromeric-banding.
Hybrids between A9 (HGPRT-) and B82 (TK-), mouse heteroploid fibroblast lines, were obtained through continuous cultivation and clonal selection; such hybrids showed marked segregation and by conventional stains displayed chromosome numbers and distribution similar to that of either parental type. Detailed analyses by Giemsa (G)- and centromeric-banding of these parental lines, and of 4 of the reduced hybrids, maintained in culture for up to 5 years, revealed the following points: (1) The distribution of the majority of individual chromosomal classes was similar for 3 of the hybrid cell lines. (2) Over two-thirds of the chromosomal arms in both the parental lines and hybrid lines were identical to normal mouse telocentric chromosomes. (3) For 2 of the hybrid lines, segregation was particularly marked with respect to those chromosomal arms whose G-banding patterns were identical to the wild-type genome; this indicated that segregation had occurred at the expense of redundant chromosomal material introduced by cell fusion. These banded studies demonstrated that segregation chiefly accounted for the sharp reduction in chromosome numbers while recombination accounted for the chromosome heterogeneity of the hybrid cells as compared to the parental genomes.
D000818:Animals; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000032:analysis; D005347:Fibroblasts / Q000648:ultrastructure; D006822:Hybrid Cells / Q000648:ultrastructure; D007621:Karyotyping; D051379:Mice; D013194:Staining and Labeling