Is aplastic anaemia due to abnormality of D.N.A.?
Lymphocytes from eleven patients with aplastic anaemia were cultured with various agents which interact with D.N.A., and the proliferative responses to phytohaemagglutinin were measured. Lymphocytes from seven of the eleven patients were unduly sensitive to bleomycin, an agent causing strand breaks in D.N.A. The findings suggest that D.N.A. in these seven patients was abnormal, possibly as a result of an abnormality of D.N.A. repair. It is suggested that in aplastic anaemia D.N.A. damage in stem cells may lead to a failure of proliferation.
D000293:Adolescent; D000328:Adult; D000368:Aged; D000741:Anemia, Aplastic / Q000097:blood / Q000209:etiology* / Q000235:genetics; D000818:Animals; D001761:Bleomycin / Q000633:toxicity; D002478:Cells, Cultured; D002648:Child; D004260:DNA Repair / Q000187:drug effects; D004277:DNA, Single-Stranded; D004317:Doxorubicin / Q000633:toxicity; D005260:Female; D006412:Hematopoietic Stem Cells / Q000473:pathology; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000473:pathology; D008297:Male; D051379:Mice; D008875:Middle Aged; D008937:Mitomycins / Q000633:toxicity
D004277:DNA, Single-Stranded; D008937:Mitomycins; D001761:Bleomycin; D004317:Doxorubicin