Human macromolecular insoluble cold globulin (MICG). I. T-cell origin of T-MICG and null cell origin of N-MICG.
Although surface immunoglobulin characterizes B cells in man, there are few surface markers that distinguish T cells. We have described a new protein synthesized in human T cells, termed T-MICG. This protein is a macromolecule of 225,000 daltons, is insoluble in the cold, and migrates as a beta-globulin on electrophoresis. Separation of human peripheral blood lymphocytes into T and B-cell populations by rosette sedimentation and anti-human-Fab columns clearly demonstrated the T-cell origin of the 225,000 dalton component. Furthermore, null cells were shown to synthesize a protein of 185,000 daltons, termed N-MICG, with physical properties similar to T-MICG, T-MICG and N-MICG were shown to be antigenically dissimilar, employing antiserum to each of these proteins. The present studies demonstrate two novel cell surface markers, T-MICG and N-MICG, which characterize T cells and null cells, respectively.
D000954:Antigens, Surface / Q000032:analysis; D001402:B-Lymphocytes / Q000032:analysis; D002462:Cell Membrane / Q000276:immunology; D003450:Cryoglobulins / Q000276:immunology / Q000302:isolation & purification*; D003602:Cytotoxicity, Immunologic; D000939:Epitopes; D006801:Humans; D008214:Lymphocytes / Q000032:analysis*; D008259:Macroglobulins / Q000276:immunology / Q000302:isolation & purification; D008970:Molecular Weight; D013601:T-Lymphocytes / Q000032:analysis*
D000954:Antigens, Surface; D003450:Cryoglobulins; D000939:Epitopes; D008259:Macroglobulins; C018090:macromolecular insoluble cold globulin