Protamine messenger RNA from rainbow trout testis contains the nucleotide sequence A-A-U-A-A-A in an untranslated region.
Full-length, complementary DNAs were prepared to rainbow trout protamine mRNA using reverse transcriptase and were labelled during synthesis by the replacement of dATP by [alpha32P]dATP or dTTP by [alpha32P]dTTP. The 32P-labelled protamine complementary DNAs were digested with T4 endonuclease IV. Fragments from the digests were separated in two dimensions, and those discrete fragments which could be identified from both the A-labelled and T-labelled complementary DNAs were subjected to sequence analysis. The sequences described here all arise from the non-coding region. One pentadecanucleotide contained the sequence A-A-U-A-A-A which has been reported by Proudfoot and Brownlee ((1976) Nature 263, 211-214) to occur in the noncoding regions of six other eukaryotic mRNAs.
D000818:Animals; D001483:Base Sequence; D003851:Deoxyribonucleases; D004720:Endonucleases; D008297:Male; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000032:analysis; D009843:Oligoribonucleotides / Q000032:analysis; D011479:Protamines / Q000096:biosynthesis*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase; D013737:Testis / Q000378:metabolism*; D014337:Trout
D009838:Oligodeoxyribonucleotides; D009843:Oligoribonucleotides; D011479:Protamines; D012333:RNA, Messenger; D012194:RNA-Directed DNA Polymerase; D003851:Deoxyribonucleases; D004720:Endonucleases