title: Preliminary characterization of isoimmunogenic placental antigens in the rabbit.
abstract: By isoimmunization of adult virgin female rabbits with rabbit placental extracts we have demonstrated the production of humoral antibody against two antigens, Ag-1 and Ag-2. Neither antigen is a normal serum protein, nor are they allotype proteins. Ag-1 has an alpha-2 mobility and a molecular weights of about 400,000. Ag-2 exhibits a gamma mobility but was not characterized. The antibody activity directed to Ag-1 in immune sera was the complement-fixing IgG fraction. Direct fluorescence-labeled antibody technique revealed that Ag-1 is a ubiquitous intracellular antigen primarily localized in the nucleus. The fast-growing, SV-40 virus-transformed rabbit kidney cell line (TRK-1) expressed much higher levels of Ag-1 than the slow-growing, non-transformed normal rabbit kidney cell line (LLC-RK1). The possibility that elevation of Ag-1 production is associated with rapid cell proliferation is discussed.
mesh_terms: D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D002460:Cell Line; D002478:Cells, Cultured; D000939:Epitopes; D005260:Female; D005455:Fluorescent Antibody Technique; D007114:Immunization; D005779:Immunodiffusion; D007074:Immunoglobulin G; D007519:Isoantigens / Q000302:isolation & purification; D010920:Placenta / Q000276:immunology*; D011247:Pregnancy; D011817:Rabbits
chemical_list: D000939:Epitopes; D007074:Immunoglobulin G; D007519:Isoantigens