Idiotype-specific neonatal suppression of phosphorylcholine-responsive B cells.
The effect on neonatal anti-idiotypic suppression on the expression of B cells of the T15 clonotype has been investigated at the level of individual clonal precursor cells. The results indicate that B cells of the T15 clonotype are almost completely eliminated from the repertoire for four months after neonatal injection of allogeneic anti-idiotypic serum. The degree of this suppression is dependent on the amount of anti-idiotypic antibody administered and is less profound if anti-idiotypic antibody is given after the first week of life. No suppression was observed when anti-idiotypic antisera were administered to mice 30 days of age or older, which may indicate that immature B cells are the population most susceptible to suppression. However, since suppression could be reversed by administration of T15 myeloma protein several days after injection of anti-idiotype, the inability to suppress adult BALB/c mice may have been due to the high level of T15 idiotype normally present in their serum. Finally, phosphorylcholine-responsive B cells of identifiable clonotypes other than T15, even a clonotype sharing antigen-combining site determinants with T15, appear unaffected by anti-T15 suppression.
D000375:Aging; D000818:Animals; D000831:Animals, Newborn / Q000276:immunology*; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology*; D002794:Choline / Q000031:analogs & derivatives*; D002999:Clone Cells / Q000276:immunology; D000939:Epitopes; D007135:Immunoglobulin Variable Region; D007165:Immunosuppression Therapy; D051379:Mice; D008807:Mice, Inbred BALB C; D009194:Myeloma Proteins / Q000276:immunology*; D010767:Phosphorylcholine / Q000276:immunology*
D000939:Epitopes; D007135:Immunoglobulin Variable Region; D009194:Myeloma Proteins; D010767:Phosphorylcholine; D002794:Choline