[Advantages in immunofluorescence of dichlortriazinylaminofluorescein conjugates isolated by single-step gel filtration].
The author describes a simple method obtaining dichlortriazinylaminofluoresceine (DCTAF) conjugates to human immunoglobulins and separation of their optimally labeled fractions. The single-stage method of the conjugate release from the nonbound flurorchrome and of a simultaneous separation of the optimally labeled fractions was carried out on Sephadex G-25 at pH 5.6--6.6 and at low ionic power of the eluate. Proteins overloaded with the dye are sorbed on gel according to their isoelectrical points.
D002850:Chromatography, Gel; D000939:Epitopes; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D006801:Humans; D007106:Immune Sera; D007136:Immunoglobulins / Q000378:metabolism; D008214:Lymphocytes / Q000276:immunology*; D008722:Methods; D014227:Triazines
D000939:Epitopes; D005452:Fluoresceins; D007106:Immune Sera; D007136:Immunoglobulins; D014227:Triazines
Preimushchestva v immunofliuorestsentsii dikhlortriazinilaminofliuorestseinovykh kon"iugatov, vydeliaemykh odnostupenchatoĭ gel'-filtratsieĭ