title: Immunologic and nonimmunologic generation of superoxide from mast cells and basophils.
abstract: Mediator release from rat peritoneal and human lung mast cells as well as human leukemic basophils was examined to determine whether super-oxide (O(-) (2)) was concomitantly generated. Immunologic or nonimmunologic stimulation of each preparation induced parallel release of histamine and O(-) (2) within 2 min. O(-) (2) production was quantitated by superoxide dismutase (SOD)-inhibitable chemiluminescence and cytochrome c reduction. SOD was detected in basophil and mast cell lysates and was also released by rat mast cells stimulated by anti-IgE. Secretory granules isolated from purified rat mast cells released histamine, O(-) (2), and SOD upon exposure to cations. Thus, both superoxide radicals and SOD may play a role in host defenses involved in immediate hypersensitivity reactions.
mesh_terms: D000818:Animals; D001491:Basophils / Q000378:metabolism*; D004734:Energy Metabolism; D006636:Histamine Release; D006801:Humans; D007109:Immunity; D007111:Immunity, Cellular; D066298:In Vitro Techniques; D008297:Male; D008407:Mast Cells / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D051381:Rats; D013482:Superoxide Dismutase / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism*
chemical_list: D013481:Superoxides; D013482:Superoxide Dismutase; D010100:Oxygen