title: Heterogeneity of Epstein-Barr virus. IV. Induction of a specific antigen by EBV from two transformed marmoset cell lines in Ramos cells.
abstract: Infection of cells of the EBV-genome-negative human B-lymphoma Ramos line with viral isolates obtained from two EBV-transformed marmoset cell lines (B95/8; Nyevu) resulted in the induction of a nuclear antigen (RAM-ag) apparently different from other EBV-associated antigen complexes. This antigen is revealed by indirect immunofluorescence and shows no detectable cross-antigenicity with EBNA or any other known EBV-associated antigen. EBV-isolates from P3HR-1 cells fail to induce a similar antigen in Ramos cells although they induce EBNA. No RAM-ag was expressed, either after infection of cells of another EBV-genome-negative human B-lymphoma line BJAB with B95-8 EBV or in a series of EBV-harbouring cell lines. Thus the antigen appears to be cell-line-specific for Ramos cells. It is also induced upon infection of either B95-8 or P3HR-1 converted Ramos sublines with EBV from B95-8 cells. All human sera with RAM-ag-reactivity revealed antibodies against VCA. However, sera from patients with acute infectious mononucleosis containing high anti-VCA-antibodies did not react with RAM-ag. Seroconversion for this antigen apparently more closely coincides with the appearance of EBNA-directed antibodies.
mesh_terms: D000818:Animals; D000914:Antibodies, Viral; D000956:Antigens, Viral; D002143:Callitrichinae; D002213:Capsid / Q000276:immunology; D002460:Cell Line; D002467:Cell Nucleus / Q000276:immunology; D002471:Cell Transformation, Neoplastic; D000939:Epitopes; D000882:Haplorhini; D004854:Herpesvirus 4, Human / Q000276:immunology*; D006801:Humans; D007163:Immunosorbent Techniques; D007181:Inclusion Bodies, Viral / Q000648:ultrastructure; D007244:Infectious Mononucleosis / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes