Determination of the number of active saprophytic aquatic bacteria by semi-continuous culture on membrane filters.
The number of bacteria in the hypolimnion waters of Lake Mikpłajski was examined by direct count on membrane filters, by the agar plate method and by semi-continuous culture on membrane filters, as previously proposed by the author. The agar plate method recovered only 20--25% of the bacteria found by direct method. On the other hand, semi-continuous culture on membrane filters allowed the recovery of about 90% of the total count. At the same time it differentiated between active, microcolony forming cells (about 70%) and inactive single cells (about 20% of the total count).
D001419:Bacteria / Q000254:growth & development*; D001431:Bacteriological Techniques; D003470:Culture Media; D008851:Micropore Filters; D014871:Water Microbiology
D003470:Culture Media