Specificity of human lymphocytes primed against allogeneic cells in vitro. 1. Optimalization of culture conditions for detection of HLA--D specificity.
The specificity of lymphocytes primed against allogeneic cells in vitro has been examined in order to find the optimal conditions for detection in the restimulating response of HLA--D specificity, as defined by homozygous cell typing. It was found that reducing the number of stimulating cells to less than the number of responding cells (ratio R/S = 4 : 1) in the priming culture, and increasing the number of stimulating cells at restimulation gave better discrimination. Priming with HLA--D heterozygous stimulating cells, where the responding and stimulating cells shared the other HLA--D determinant, rendered the primed cells somewhat more discriminatory than priming with HLA--D homozygous cells. The optimal length of the secondary culture was found to be about 38 h.
D001402:B-Lymphocytes / Q000276:immunology; D000939:Epitopes; D006680:HLA Antigens; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000276:immunology*; D009000:Monocytes / Q000276:immunology
D000939:Epitopes; D006680:HLA Antigens