Affinity chromatographic purification of the pregnancy zone protein.
An immunospecific affinity chromatographic method for purification of human pregnancy zone protein (PZP) directly from serum is described. Highly purified goat-anti-human PZP-immunoglobulin was applied as a ligand. Recovery of PZP varied from 56--75%. The impurities constituted maximally 5--10% of the total protein in the eluate. The purification factor was approximately 100.
D001798:Blood Proteins / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D005260:Female; D006801:Humans; D011247:Pregnancy; D011263:Pregnancy Trimester, Third; D000511:alpha-Macroglobulins / Q000032:analysis*
D001798:Blood Proteins; D000511:alpha-Macroglobulins