Staining calcified tissues in plastic embedding medium.
Two procedures for staining sections of calcified teeth and/or bone embedded in plastic without dissolving the embedding medium were developed to study early bone regeneration in proximity to carbon implants in monkey alveolar bone and tissue degeneration in pathological human bone. These procedures allow differential staining of delicate bone tissues and prevent breakage of sections because the supporting plastic does not have to be removed.
D000818:Animals; D001862:Bone Resorption; D001842:Bone and Bones / Q000033:anatomy & histology*; D004396:Coloring Agents; D004801:Eosine Yellowish-(YS); D000882:Haplorhini; D006416:Hematoxylin; D006801:Humans; D008253:Macaca mulatta; D010012:Osteogenesis; D010024:Osteoporosis / Q000473:pathology; D010969:Plastics; D011309:Preservation, Biological; D013194:Staining and Labeling / Q000379:methods*; D014070:Tooth / Q000033:anatomy & histology*; D014992:Xylenes
D004396:Coloring Agents; D010969:Plastics; D014992:Xylenes; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin