A myeloma hybrid producing antibody specific for an allotypic determinant on "IgD-like" molecules of the mouse.
A hybrid cell line was produced by fusing a mouse myeloma line with spleen cells from BALB/c mice immunized with B10 cells. The hybrid line grew in tissue culture and in syngeneic mice and produced IgM antibody specific for "IgD-like" molecules of mice with the Igb haplotype. The concentration of monoclonal antibody in the serum of tumor-bearing animals reached about 2 mg/ml and gave cytotoxic titers of up to 1:800 000. The derivation of the line, some properties of the antibody secreted and the nature of its antigenic target are described.
D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D002459:Cell Fusion; D002999:Clone Cells / Q000276:immunology; D000939:Epitopes; D005455:Fluorescent Antibody Technique; D014644:Genetic Variation; D006824:Hybridization, Genetic; D007072:Immunoglobulin D; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D009101:Multiple Myeloma / Q000276:immunology*; D011947:Receptors, Antigen, B-Cell
D000939:Epitopes; D007072:Immunoglobulin D; D011947:Receptors, Antigen, B-Cell