Human tumor and rodent-human hybrid cells with an increased number of active human NORs.
A human fibrosarcoma line, HT1080-6TG, with a near diploid number of chromosomes, has an average of 7.3 chromosomes with an Ag-stained nucleolus organizer region (NOR). Cells of this line with an increased number of chromosomes have an increased number of Ag-stained NORs. This cell line has been used as the human parent in constructing mouse-human and rat-human hybrids that segregate rodent chromosomes. The hybrid ccell lines, which have 100 or more chromosomes per cell, show a proportionate increase in the number of Ag-stained NORs (means, 11.4--16.8). The frequency of association of acrocentric chromosomes increases in a similar fashion. There is no evidence of inactivation of human NORs in these cells.
D000818:Animals; D002452:Cell Count; D002460:Cell Line; D002466:Cell Nucleolus / Q000648:ultrastructure*; D005354:Fibrosarcoma / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells / Q000648:ultrastructure*; D051379:Mice; D051381:Rats; D012834:Silver; D013194:Staining and Labeling
D012834:Silver