Detection of B-cell-specific alloantibodies in pregnancy sera in the lymphocytotoxicity and the indirect immunofluorescence techniques.
Two hundred and eight pregnancy sera were tested for the presence of antibodies specific for lymphocyte sub-populations by using the isolated B and T lymphocytes from the women's mating partners. This was done by the microlymphocytotoxicity and the indirect immunofluorescence techniques. Five sera (2.5%) reacted exclusively with B lymphocytes and sixty-three sera (30.2%) reacted with both B and T lymphocytes; none of the sera was specific for T cells. Several sera, reacting with both B and T lymphocytes, were absorbed with platelets and this procedure revealed nine additional antiseraa specific for B lymphocyte antigens. Specificity studies on a panel of forty-eight HLA-ABCD typed individuals indicated that most antisera possibly defined new B-cell antigens. Family studies established that the antigens defined by these antiser were coded for by genes in the Major Histocompatibility Complex.
D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000276:immunology*; D002469:Cell Separation; D002874:Chromosome Mapping; D003601:Cytotoxicity Tests, Immunologic; D000939:Epitopes; D005260:Female; D005455:Fluorescent Antibody Technique; D006680:HLA Antigens; D006801:Humans; D007106:Immune Sera; D007231:Infant, Newborn; D007518:Isoantibodies / Q000032:analysis*; D008297:Male; D011247:Pregnancy; D011995:Recombination, Genetic; D013601:T-Lymphocytes / Q000276:immunology
D000939:Epitopes; D006680:HLA Antigens; D007106:Immune Sera; D007518:Isoantibodies