Distribution of immunoglobulin determinants on the surface of Xenopus laevis splenic lymphocytes.
The distribution of surface immunoglobulin (Ig) determinants on Xenopus laevis splenic lymphocytes after combination with divalent rabbit anti-Ig coupled to ferritin was studied. The electron micrographs showed the presence of immune complexes in 67% of lymphocytes treated at 0 degrees C-4 degrees C. The complexes were located all around the membrane and uniformly distributed in a random fashion. The variation of ferritin grain counts on cell sections is such, that the existence of two major subclasses of Ig-positive cells may be suggested. Raising the temperature produced a rapid interiorization of the complexes in vesicles without any previous aggregation to form a "cap" having occurred.
D000818:Animals; D000936:Antigen-Antibody Complex; D000939:Epitopes; D005455:Fluorescent Antibody Technique; D007074:Immunoglobulin G / Q000032:analysis*; D008214:Lymphocytes / Q000276:immunology*; D013154:Spleen / Q000276:immunology*; D014981:Xenopus / Q000276:immunology*
D000936:Antigen-Antibody Complex; D000939:Epitopes; D007074:Immunoglobulin G