title: Immunoreactivity and receptor binding of mixed recombinants of human growth hormone and chorionic somatomammotropin.
abstract: The behavior in six radioligand assays of the recombinant obtained by the noncovalent complementation of the reduced and carbamoylmethylated 134-residue amino-terminal fragment of human growth hormore with the reduced and carbamoylmethylated 51-residue carboxyl-terminal fragment of human chorionic somatomammotropin was compared to that of the analogous recombinant of the 133-residue amino-terminal fragment of human chorionic somatomammotropin and the 51-residue carboxyl-terminal fragment of human growth hormone. The determinants for the hepatic growth hormone receptor binding and for the lactogenic receptor binding of human growth hormone are on the amino-terminal fragments. The antigenic determinants for both a monospecific antiserum to human growth hormone and a monospecific antiserum to human chorionic somatomammotropin also are on the amino-terminal fragments of their respective antigens. The mixed recombinant of the amino-terminal fragment of human growth hormone with the carboxyl-terminal fragment of human chorionic somatomammotropin retains full radioimmuno- and radioreceptor activity after lyophilization.
mesh_terms: D000939:Epitopes; D005341:Fibrinolysin; D013006:Growth Hormone; D006801:Humans; D007700:Kinetics; D010446:Peptide Fragments; D010928:Placental Lactogen; D011485:Protein Binding; D011863:Radioimmunoassay; D011869:Radioligand Assay
chemical_list: D000939:Epitopes; D010446:Peptide Fragments; D013006:Growth Hormone; D010928:Placental Lactogen; D005341:Fibrinolysin