[Immunochemical study of the water-soluble lens proteins in the embryo of Xenopus laevis with the mutation of periodic albinism].
The crystallins of ap mutants of Xenopus laevis have been studied in comparison with those of normal embryos and adults using the complex of immunochemical methods (immunoelectrophoresis, immunodiffusion, immunoadsorption, immunofluorescence, isoelectrofocusing with immunoidentification). The analysis was carried out with antisera to electrophoretic fractions of the mutant lens. 11 organ-specific antigens were found in the lens of both the normal and mutant animals. These proteins are heterogenous by electrophoretic mobility, isoelectrical point, antigenic and species specificity. Each class of crystallins contains antigens which are specific: a) for amphibians only, b) for lower vertebrates, c) for vertebrates in general. No qualitative differences were found between crystallins of the normal and mutant animals. Immunofluorescence analysis has shown that crystalins appear in the normal and mutant embryos practically at the same time. No significant differences in the appearance of specific immunofluorescence between the normal and mutant embryos were found (with various antisera). gamma-crystallins and, perhaps, a part of the primary lens fibers. Alpha-crystallins appear later. gamma-crystallins are first identified the synthesis of which manifests itself at the advanced developmental stages. The quantitative predominance of some beta--gamma-crystallins in the mutant lens detected by us (electrophoresis, isoelectrofocusing) is not related to their earlier synthesis in the embryogenesis.
D000417:Albinism / Q000196:embryology*; D000818:Animals; D000941:Antigens / Q000032:analysis; D003459:Crystallins / Q000276:immunology*; D000939:Epitopes; D009154:Mutation; D010507:Periodicity; D012995:Solubility; D013045:Species Specificity; D014981:Xenopus / Q000196:embryology*
D000941:Antigens; D003459:Crystallins; D000939:Epitopes
Immunokhimicheskoe izuchenie vodorastvorimykh belkov khrustalika u zarodysha Xenopus laevis s mutatsieĭ periodicheskogo al'binizma.