title: Characterization of a retravirus isolated from squirrel monkeys.
abstract: A new retravirus (SMRV) isolated from a squirrel monkey, Saimiri sciureus, has an Mg2+-dependen reverse transcriptase and a buoyant density of 1.17 g/cm3 in sucrose and 1.21 g/cm3 in cesium chloride, similar to the mouse mammary tumor virus and the Mason-Pfizer monkey virus. The polypeptide patter of SMRV as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis was distinct from the reported polypeptide patterns of known retraviruses. Four major polypeptides of molecular weights 40,000, 20,000, 14,000 and 8,000 were resolved in virus propagated in human, mink, and canine cells. In A204 human rhabdomyosarcoma cells, a protein of 73,000 daltons (gp73) represented the major viral glycoprotein as determined by [3H]glucosamine labeling. Additional proteins were also observed, but their presence depended on the cell type in which the virus was propagated. In both species-and interspecies-specific assays, no antigenic relatedness was observed between SMRV and Mason-Pfizer monkey virus, mouse mammary tumor virus, baboon endogenous virus (BaLV), woolly monkey virus (SSV-1), murine leukemia virus, endogenous feline type C virus (RD-114), bovine leukemia virus, and equine infectious anemia virus. These findings indicate that SMRV represents a new retravirus and the first isolate from a New World monkey.
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D004285:Dogs; D000939:Epitopes; D000882:Haplorhini; D006801:Humans; D008274:Magnesium / Q000494:pharmacology; D008970:Molecular Weight; D009858:Oncogenic Viruses / Q000032:analysis / Q000201:enzymology / Q000254:growth & development; D010455:Peptides / Q000032:analysis; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D012453:Saimiri; D014764:Viral Proteins / Q000032:analysis / Q000276:immunology; D014776:Virus Cultivation
chemical_list: D000956:Antigens, Viral; D000939:Epitopes; D010455:Peptides; D014764:Viral Proteins; D012194:RNA-Directed DNA Polymerase; D008274:Magnesium