Induction of immunological tolerance requires that the B cells can respond to the polyclonal B-cell-activating properties of the thymus-independent antigens.
Mice were rendered specifically tolerant to the fluorescein isothiocyanatedextran (FITC) epitope by injection of FITC-dextran B512. Their spleen cells were removed at various times and cultivated in vitro with different polyclonal B-cell activators, such as lipopolysaccharide (LPS), purified protein derivative of tuberculin, and native dextran. LPS caused the appearance of high affinity anti-FITC plaque-forming cells to an equal extent with cells from untreated and tolerant animals, whereas native dextran failed to activate cells from tolerant mice, although it was a potent activator of normal cells. It was concluded that tolerance induction only affects those B cells that could respond to the polyclonal B-cell-activating properties of the tolerogen, but not other B cells having an identical set of Ig receptors directed against the tolerogen.
D000818:Animals; D000921:Antibody-Producing Cells; D000941:Antigens; D001402:B-Lymphocytes / Q000276:immunology*; D001666:Binding Sites, Antibody; D003911:Dextrans / Q000276:immunology / Q000494:pharmacology; D000939:Epitopes; D005452:Fluoresceins / Q000276:immunology; D006462:Hemolytic Plaque Technique; D007108:Immune Tolerance / Q000187:drug effects*; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D013861:Thiocyanates / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology*; D014373:Tuberculin
D000941:Antigens; D003911:Dextrans; D000939:Epitopes; D005452:Fluoresceins; D008070:Lipopolysaccharides; D013861:Thiocyanates; D014373:Tuberculin