Serological identification of neoantigens on mouse fibroblasts which have undergone "spontaneous" malignant alteration in vitro.
ST-L1 is a cell line established from lung explants from a normal ST/a mouse. The ST-L1 cells have undergone spontaneous malignant alteration in vitro. The cells were rejected after inoculation into syngeneic immunocompetent hosts, and a syngeneic humoral immuneresponse against the ST-L1 cells has been detected. The specificity of this humoral response was investigated. The syngeneic response to ST-L1 was characterized by indirect immunofluorescence tests and by immunoprecipitation of radiolabelled cells and of a C-type virus produced by the cell lines. The specific anti-ST-L1 reactivities were found to be directed against the envelope glycoprotein of an endogenous C-type virus expressed by the antigenic cell line.
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D011232:Chemical Precipitation; D003429:Cross Reactions; D000939:Epitopes; D005347:Fibroblasts / Q000032:analysis / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D051379:Mice; D011863:Radioimmunoassay; D012190:Retroviridae / Q000276:immunology / Q000302:isolation & purification; D014764:Viral Proteins / Q000032:analysis
D000939:Epitopes; D007106:Immune Sera; D014764:Viral Proteins