title: Further evidence for the involvement of base and base sequence in the antigenic determination of thermally denatured DNA.
abstract: The antigenic determinant of thermally denatured salmon sperm (ss) DNA was extensively investigated with a radioimmunoassay technique using the ssDNA immune system. It was demonstrated that mixed sequences of purines and pyrimidines play a key role in the antigenic determination of DNA. On the other hand, the importance of the bases in the manifestation of DNA antigenicity was demonstrated by using brominated, methylated and/or ultraviolet irradiated ssDNA preparations.
mesh_terms: D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000276:immunology*; D000939:Epitopes; D008297:Male; D009691:Nucleic Acid Denaturation; D009841:Oligonucleotides / Q000494:pharmacology; D011687:Purines; D011743:Pyrimidines; D011863:Radioimmunoassay; D012474:Salmon / Q000276:immunology; D013094:Spermatozoa / Q000276:immunology
chemical_list: D000939:Epitopes; D009841:Oligonucleotides; D011687:Purines; D011743:Pyrimidines; D004247:DNA