Production of "rapid-harvest" Moloney murine leukemia virus by continuous cell culture on synthetic capillaries.
Moloney murine leukemia virus was harvested automatically within 60 min of release from chronically infected NIH/3T3 cells (clone 1) cultured on bundles of synthetic capillaries. Production of virus as measured by a determination of reverse transcriptase activity and by the XC syncytia assay demonstrated that highly infectious "rapid-harvest" virus was recovered from NIH/3T3 cells (clone 1) grown for periods of up to 10 days.
D002460:Cell Line; D002999:Clone Cells; D003584:Cytological Techniques; D008979:Moloney murine leukemia virus / Q000254:growth & development* / Q000472:pathogenicity; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D014776:Virus Cultivation / Q000295:instrumentation*
D012194:RNA-Directed DNA Polymerase