Purification of placental alpha-1-foetoprotein.
Quantitation of placental alpha-1-foetoprotein was done by the radioimmunoassay technique and gave a mean value of 6060 +/- 22.2 ng/g fresh tissue. The purification process included three methods. (1) Protein precipitation was performed using ammonium sulphate at 50 and 70% saturation. Elution on a Concanavalin A-sepharose column was used to diminish the interference of albumin with alpha-foetoprotein. (2) A coupling immunoadsorption technique using CNBr-activated Sepharose 4-B, antialbumin and antitransferrin, was found to be more reproducible. (3) Counter-immunoelectrophoresis and discontinuous gel electrophoresis gave a 60% yield with a 400-fold purification.
D002846:Chromatography, Affinity; D003378:Counterimmunoelectrophoresis; D004589:Electrophoresis, Disc; D005260:Female; D006801:Humans; D007163:Immunosorbent Techniques; D010920:Placenta / Q000032:analysis*; D011247:Pregnancy; D011863:Radioimmunoassay / Q000379:methods; D000509:alpha-Fetoproteins / Q000302:isolation & purification*
D000509:alpha-Fetoproteins