Characterization of a monoclonal anti-beta 2-microglobulin antibody and its use in the genetic and biochemical analysis of major histocompatibility antigens.
A monoclonal anti-beta 2-microglobulin (BBM.1 antibody) was produced by cell fusion between the mouse myeloma, P3-X63-Ag8, and spleen cells from a BALB/c mouse immunized with Molt 4, a human T cell line. BBM.1 antibody was fully inhibited by soluble beta 2-microglobulin and purified HLA-A, B antigens and reacted with human-mouse somatic cell hybrids only if they had chromosome 15 and expressed human beta 2-microglobulin. It was cytotoxic in complement-dependent lysis and of the IgG class. BBM.1 and a monoclonal anti-HLA-A, B, C glycoprotein antibody, W6/32 (Barnstable, C. J. et al., Cell 1978. 14:9.), were used to quantitate relative amounts of beta 2-microglobulin and HLA-A, B, C glycoproteins on different human cell types. Thymocytes and the Molt 4 cell line showed a considerable excess of beta 2-microglobulin over HLA-A, B, C glycoproteins, as measured by W6/32 reactivity. B cell lines, peripheral blood lymphocytes, fibroblasts, a HeLa cell derivative, and HSB2, another T cell line, had equal amounts. Immunological cross-reactions between HLA-A, B, C antigens and beta 2-microglobulin and their homologues in other species were detected with the BBM.1 and W6/32 antibodies. The W6/32 antigenic determinant appears to be more highly conserved than that recognized by the BBM.1 antibody.
D000818:Animals; D000906:Antibodies / Q000145:classification; D000921:Antibody-Producing Cells / Q000378:metabolism; D001609:Beta-Globulins / Q000276:immunology*; D055598:Chemical Phenomena; D002621:Chemistry; D002999:Clone Cells / Q000276:immunology; D000939:Epitopes; D006023:Glycoproteins / Q000276:immunology; D006680:HLA Antigens / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D011817:Rabbits; D013045:Species Specificity; D001613:beta 2-Microglobulin / Q000276:immunology*
D000906:Antibodies; D001609:Beta-Globulins; D000939:Epitopes; D006023:Glycoproteins; D006680:HLA Antigens; D001613:beta 2-Microglobulin