Antigen-specific regulation of myeloma cell differentiation in vivo by carrier-specific T cell factors and macrophages.
Previous studies demonstrated that: i) the TNP-binding myeloma MOPC-315 differentiated during in vivo growth in diffusion chambers (DC) implanted i.p. into normal BALB/c mice, and ii) the myeloma cell differentiation was regulatable by carrier-specific presentation of TNP to MOPC-315 cells in carrier-primed mice. In those studies, promotion and suppression of MOPC-315 differentiation occurred in the presence of carrier-specific helper and suppressor activities, respectively. In the present studies, we demonstrate that carrier-specific regulation of MOPC-315 differentiation can be adoptively transferred to normal mice with carrier-primed T lymphocytes. In addition, the induced regulation of MOPC-315 differentiation is abrogated when macrophages are not present with MOPC-315 cells in the DC. These studies establish the immunologic basis of myeloma cell regulation and suggest that soluble, carrier-specific helper and suppressor factors of T cell origin regulate MOPC-315 differentiation directly or in collaboration with macrophages.
D000818:Animals; D002352:Carrier Proteins / Q000276:immunology*; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D000939:Epitopes; D007116:Immunization, Passive; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010954:Plasmacytoma / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D002352:Carrier Proteins; D000939:Epitopes