The covalent coupling of proteins to periodate-oxidized sephadex: a new approach to immunoadsorbent preparation.
Proteins were covalently coupled in good yield to periodate-oxidized Sephadex. The reaction was studied in detail with respect to Sephadex type, sodium m-periodate concentration, protein (albumin or immunoglobulin) concentration, time course, temperature dependence, and pH optimum. The complexes were stable and were used successfully for the immunoadsorption of sheep anti-rabbit immunoglobulin antibodies.
D000818:Animals; D003911:Dextrans; D007074:Immunoglobulin G; D007163:Immunosorbent Techniques; D010503:Periodic Acid-Schiff Reaction; D011485:Protein Binding; D011817:Rabbits; D012710:Serum Albumin, Bovine; D013194:Staining and Labeling
D003911:Dextrans; D007074:Immunoglobulin G; D012710:Serum Albumin, Bovine