Preparation of anti-etiocholenic acid antiserum and anti-cholesterol succinate antiserum and their cross reactivities.
Anti-3-hydroxy 5-androstene carbonic acid (etiocholenic acid) antiserum was obtained by immunizing rabbits with anetiocholenic acid-polylysine complex. Anticholesterol succinate antiserum was also prepared by immunizing rabbits with a cholesterol succinate-polylysine complex. The cross reactivities of these antisera were examined by means of radioimmunoassay system, immunolysis of liposome and passive hemagglutination reaction. Anti-etiocholenic acid antiserum was specific against the structure of ring A of cholesterol and the nature of OH group at C3 position and anti-cholesterol succinate antiserum was specific against the side chain similar to that of cholesterol.
D000737:Androstenols / Q000276:immunology*; D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D002352:Carrier Proteins / Q000276:immunology; D002784:Cholesterol / Q000031:analogs & derivatives*; D002788:Cholesterol Esters / Q000276:immunology*; D003429:Cross Reactions; D000939:Epitopes; D006385:Hemagglutination Inhibition Tests; D008081:Liposomes / Q000276:immunology; D011817:Rabbits; D011863:Radioimmunoassay; D013386:Succinates / Q000276:immunology
D000737:Androstenols; D002352:Carrier Proteins; D002788:Cholesterol Esters; D000939:Epitopes; D008081:Liposomes; D013386:Succinates; D002784:Cholesterol