Separation of beta2-microglobulin from transfer factor in dialyzable leukocyte extracts.
Dialyzable leukocyte extracts prepared according to the original method of LAWRENCE contain 4 X 10(4) molecules of beta2-microglobulin per lymphocyte equivalent. The negative skin test converting biological activity (transfer factor) is separable by means of Sephadex G-25 gel chromatography from the beta2-microglobulin component of the extracts. This finding does not support the hypothesis of SHIFRINE and SCIBIENSKI on beta2-microglobulin being the nonspecific anchor of specific transfer factor to nonsensitized lymphocytes.
D001609:Beta-Globulins / Q000302:isolation & purification*; D006801:Humans; D007962:Leukocytes / Q000276:immunology; D014020:Tissue Extracts; D014165:Transfer Factor / Q000032:analysis*; D001613:beta 2-Microglobulin / Q000302:isolation & purification*
D001609:Beta-Globulins; D014020:Tissue Extracts; D014165:Transfer Factor; D001613:beta 2-Microglobulin