The effect of human serum on 3H-thymidine incorporation in human prostate tumors in tissue culture.
Human prostatic tumors, 4 adenomas and 4 adenocarcinomas, were established in tissue culture. We used the incorporation of 3H-thymidine into deoxyribonucleic acid as a measure of cell activity to compare the effects of supplementing the culture media with human or fetal calf serum. 3H-thymidine incorporation was greater with human serum, with up to 120 per cent increase observed. The addition of dihydrotestosterone (10(-8)M.) did not enhance or inhibit 3H-thymidine incorporation.
D000818:Animals; D001769:Blood; D002417:Cattle; D046508:Culture Techniques; D004247:DNA / Q000096:biosynthesis*; D013196:Dihydrotestosterone / Q000097:blood / Q000494:pharmacology; D006801:Humans; D008297:Male; D011470:Prostatic Hyperplasia / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000378:metabolism*; D013739:Testosterone / Q000097:blood / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism*; D014316:Tritium
D013196:Dihydrotestosterone; D014316:Tritium; D013739:Testosterone; D004247:DNA; D013936:Thymidine