[The spleen in "nude" mice: an immunological and immunocytochemical study (author's transl)].
An immunological and immunocytochemical study to compare spleen cells of Nude (C57B1) and Swiss mice is reported. The percentage of surface immunoglobulins bearing lymphocytes is identical in both strains of animal (40%). An activated C3 receptor is present in the majority of these cells and has been demonstrated within the germinal centers in both cell suspensions and frozen tissue sections. The population of Fc receptor bearing cells is more heterogenous, but the percentage of these is nearly identical in both Swiss and Nude mice. T-cell specificity is identified with an anti-thymocyte and an antibrain antiserum using an indirect immunoperoxidase method. The percentage of cells detected with the anti-thymocyte antiserum is very low in the spleen of Nude mice (5%) compared to that in the Swiss mice (42%). In the Nude mice, the anti-brain antiserum detects up to 30% of the spleen cells which may be considered as precursors and, so far, these 30% of cells in the Nude mice are considered as "null" cells, compared to only 20% in normal mice. The localization of the various immunocompetent cells within the spleen tissue is determined by immunofluorescence, immunoenzymology and the EAC rosette test. This study leads to the conclusion that in the periarteriolar areas of the spleen of the Nude mice, immune cells are sparse but that thymo-independent cells are located in the same region in both species of mice.
D015100:3,3'-Diaminobenzidine; D000818:Animals; D003176:Complement C3 / Q000378:metabolism; D005260:Female; D005455:Fluorescent Antibody Technique; D007104:Immune Adherence Reaction; D007106:Immune Sera / Q000494:pharmacology; D007124:Immunoenzyme Techniques; D007141:Immunoglobulin Fc Fragments / Q000032:analysis; D008221:Lymphoid Tissue / Q000166:cytology; D008297:Male; D051379:Mice; D008819:Mice, Nude; D010586:Phagocytes / Q000276:immunology; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D011955:Receptors, Drug; D013154:Spleen / Q000276:immunology*; D013194:Staining and Labeling; D013601:T-Lymphocytes / Q000276:immunology
D003176:Complement C3; D007106:Immune Sera; D007141:Immunoglobulin Fc Fragments; D011947:Receptors, Antigen, B-Cell; D011955:Receptors, Drug; D015100:3,3'-Diaminobenzidine
La rate de souris "nude": étude immunologique et immunocytochimique