Plasmacytoma spleen colonization: a sensitive, quantitative in vivo assay for idiotype-specific immune suppression of MOPC-315.
A spleen colony-forming assay for the measurement of idiotype-specific transplantation resistance to MOPC-315 is described. The assay is highly quantitative, sensitive, reproducible, less time consuming and distinctly superior to conventional in vivo assays which measure tumor incidence, tumor size, and/or host survival time after tumor challenge. The assay directly measures those clonogenic cells of the MOPC-315 myeloma which have a sufficient proliferative capacity to form macroscopic splenic foci within 14 days after intravenous challenge.
D000818:Animals; D002478:Cells, Cultured; D004306:Dose-Response Relationship, Immunologic; D000939:Epitopes; D007165:Immunosuppression Therapy; D051379:Mice; D008807:Mice, Inbred BALB C; D009194:Myeloma Proteins / Q000276:immunology; D009368:Neoplasm Transplantation; D010954:Plasmacytoma / Q000276:immunology*; D013154:Spleen / Q000276:immunology*
D000939:Epitopes; D009194:Myeloma Proteins