Purification, biochemical characterization and serological analysis of cellular deoxyribonucleic acid polymerases and a reverse transcriptase from spleen of a patient with myelofibrotic syndrome.
The present study describes the separation and purification of a reverse transcriptase and cellular DNA polymerases from the human spleen of a patient with myelofibrotic syndrome. The specific requirements with respect to bivalent cations and template-primers for DNA polymerase-alpha, DNA polymerase-beta and DNA polymerase-gamma, as well as for the reverse transcriptase, are reported. Sedimentation-velocity measurements of the purified enzymes gave values of 150000, 40000, 100000 and 70000 daltons for DNA polymerase-alpha DNA polymerase-beta, DNA polymerase-gamma and the reverse transcriptase respectively. Serological studies have shown that the reverse transcriptase from human spleen is not antigenically related to cellular DNA polymerase-alpha, -beta or -gamma, but is antigenically related to reverse transcriptase from simian sarcoma virus and gibbon-ape leukaemia virus.
D002675:Child, Preschool; D002845:Chromatography; D004259:DNA-Directed DNA Polymerase / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D005260:Female; D006801:Humans; D008970:Molecular Weight; D055728:Primary Myelofibrosis / Q000201:enzymology* / Q000276:immunology; D012194:RNA-Directed DNA Polymerase / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D013154:Spleen / Q000201:enzymology* / Q000276:immunology; D013577:Syndrome; D013698:Templates, Genetic
D012194:RNA-Directed DNA Polymerase; D004259:DNA-Directed DNA Polymerase