Isolation and antigenic characterization of the product of a third polymorphic H-2 locus, H-2L.
The serology, immunochemistry, and genetics of the product(s) of a third H-2 locus, H-2L (previously designated D') have been studied by using an antiserum raised in BALB/c H-2db mutant mice against tissues from the wild type strain, BALB/c. Genetic mapping studies and sequential immunoprecipitation experiments both indicate that this antiserum reacts specifically with L molecules. These results imply that an H-2L product is antigenically undetectable in BALB/c-H-2db mice and that the lesion in this mutant is confined to the H-2L and not the H-2D locus. Two new specificities, H-2.64 and H-2.65, are defined by the reactivity of anti-L serum on allogeneic cells, and the strain distribution of these specificities suggests the existence of at least three H-2L alleles. This third H-2 locls is therefore polymorphic and in view of this and other similarities to the H-2K and H-2D loci, it must be considered in any evolutionary models dealing with the origin of multiple subloci of the major histocompatibility complex.
D000818:Animals; D002874:Chromosome Mapping; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes; D006023:Glycoproteins; D006649:Histocompatibility Antigens / Q000302:isolation & purification*; D007106:Immune Sera / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D011110:Polymorphism, Genetic; D014176:Protein Biosynthesis
D000939:Epitopes; D006023:Glycoproteins; D006649:Histocompatibility Antigens; D007106:Immune Sera