An improved acriflavine-Feulgen method.
The acriflavine-Feulgen method for the histochemical demonstration of deoxyribonucleic acid was modified by staining hydrolyzed cells with 0.01% acriflavine dissolved in 90% ethanol. This method offered the following advantages: (a) it simplified the preparation of the acriflavine-Feulgen reagent; (b) it left the cytoplasm essentially unstained while staining the nuclei bright green in hydrolyzed cells and left the cytoplasm and nuclei essentially unstained in unhydrolyzed cells; (c) it eliminated poorly defined reagents from the staining solutions. Because of these staining properties, this technique may be especially useful in the quantitative determination of deoxyribonucleic acid by cytofluorometry.
D000166:Acridines; D000167:Acriflavine; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D003593:Cytoplasm / Q000378:metabolism; D004247:DNA / Q000032:analysis / Q000378:metabolism*; D000431:Ethanol; D006651:Histocytochemistry; D006868:Hydrolysis; D013194:Staining and Labeling
D000166:Acridines; D000167:Acriflavine; D000431:Ethanol; D004247:DNA