Immunochemical studies of myelin basic protein in shiverer mouse devoid of major dense line of myelin.
The myelin-deficient mutant Shiverer (Shi/Shi) lacks basic protein (MBP) in the myelin of its central nervous system (CNS). Less than 3% of the normal content in MBP is present in a brain extract of Shi/Shi as determined by radioimmunoassay. Indirect immunofluorescence is negative when using specific anti-MBP serum. The importance of Shi/Shi (as compared to other hypomelinating mutants) stems from the specificity of this genetic lesion, i.e. the lack of basic protein.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D001923:Brain Chemistry; D005455:Fluorescent Antibody Technique; D051379:Mice; D008818:Mice, Neurologic Mutants / Q000378:metabolism*; D004676:Myelin Basic Protein / Q000032:analysis / Q000378:metabolism*; D011863:Radioimmunoassay
D004676:Myelin Basic Protein