[Ability of FAB-fragments of normal rabbit and human gamma globulins to suppress human lymphocyte blast transformation induced by phytomitogens].
Monovalent and bivalent Fab-fragments of normal human or rabbit gamma-globulin suppressed blasttransformation of human lymphocytes induced by phytohemagglutinin and concanavalin A. Peptic F(ab)2-fragments from highly-purified rabbit anti-DNP antibody displayed suppressing activity similar to that of the fragments of normal gamma-globulin. Fab-fragments affected blasttransformation when added to lymphocytes either simultaneously with the PHA or 24 and 48h after the mitogen. The data obtained may indicate that the inhibiting of lymphocyte blasttransformation produced by the gamma-globulin fragments was not caused by their competing with mitogens for the receptors on the target-cell; the Fab-fragment activity was probably determined by the structures located outside the antibody active centre.
D000818:Animals; D000937:Antigen-Antibody Reactions; D006801:Humans; D007140:Immunoglobulin Fab Fragments; D007165:Immunosuppression Therapy; D037102:Lectins / Q000276:immunology*; D008213:Lymphocyte Activation / Q000187:drug effects*; D011817:Rabbits; D013997:Time Factors; D005719:gamma-Globulins / Q000276:immunology*
D007140:Immunoglobulin Fab Fragments; D037102:Lectins; D005719:gamma-Globulins
Sposobnost' FAB-fragmentov normal'nykh gamma-globulinov krolika i cheloveka podavliat' blasttransformatsiiu limfotsitov cheloveka, indutsirovannuiu fitomitogenami.