A simple fluorescence staining technique for the differentiation of human tissue transplanted into nude mice.
Human and mouse nuclei can be distinguished by differences in the constitutive heterochromatin when stained with quinacrine dihydrochloride. With the staining method described, mouse heterochromatin during interphase appears as brilliant fluorescent chromocenters. By replacing the commonly used aqueous buffer mounting medium with a xylene-diluted synthetic resin, the haziness of the nuclear fluorescence is eliminated thus allowing identification of the heterochromatin pattern in histological preparations. A requirement for the definite identification of cells of human or murine origin in the nude mouse is the knowledge that the heterochromatin arrangements changes according to the stage of differentiation of the cell of the position of a particular nucleus within the cell cycle.
D000818:Animals; D005260:Female; D005456:Fluorescent Dyes; D006570:Heterochromatin / Q000032:analysis; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010053:Ovary / Q000637:transplantation*; D011797:Quinacrine Mustard; D013194:Staining and Labeling / Q000379:methods*; D013737:Testis / Q000637:transplantation*; D014183:Transplantation, Heterologous
D005456:Fluorescent Dyes; D006570:Heterochromatin; D011797:Quinacrine Mustard