[Isolation of the membranes of Actinomyces sp. 26-115, a producer of actinomycin C].
A summation fraction of the membranes of Actinomyces sp. 26-115 was obtained as a result of lysis of its protoplasts in a hypotonic medium. The qualitative content of protein, lipids, phospholipids, nucleic acids, glucosamine and muramic acid was determined in the membranes at various stages of the organism development. Phosphatidylcholine is the main component of phospholipids in this organism. Intracellular actinomycin was found inside the protoplasts. Electrophoregrams of the microprotoplasts and membranes are presented. Actinomycin was also detected in the membranes. Still, it is not clear whether it is a component of the membrane or it is adsorbed on the membrane during the process of its isolation. The final conclusion on the relationship between the membrane and localization of actinomycin in the cell requires further investigation.
D000190:Actinomyces / Q000302:isolation & purification*; D002462:Cell Membrane / Q000032:analysis / Q000648:ultrastructure; D003609:Dactinomycin / Q000096:biosynthesis*; D008854:Microscopy, Electron; D011523:Protoplasts / Q000032:analysis / Q000648:ultrastructure; D013997:Time Factors
D003609:Dactinomycin
Poluchenie membran Actinomyces sp. 26-115, produtsenta.