Antigenic difference between serum amyloid protein SSA and its low molecular weight derivative SAAL revealed by an anti-amyloid A antiserum at different temperatures.
Serum amyloid protein SAA and its low molecular weight derivate SAAL have been compared in immunodiffusion with an antiserum to amyloid A at different temperatures. At room temperature or at 37 degree C SAAL and SAA did precipitate with anti-AA forming a line of identity. At 4 degrees C, however, only SAAL did react with anti AA. Experiments are described which show that the SAA-derivative SAAL has AA-related antigenic determinants exposed whereas these determinants are hidden in the parent SAA and are only exhibited as result of an unknown temperature-dependent process. Therefore, SAAL can also be distinguished from SAA by the availability of AA-related antigenic determinants for anti-AA antibodies at 4 degrees C.
D000682:Amyloid / Q000276:immunology*; D000941:Antigens; D000939:Epitopes; D006689:Hodgkin Disease / Q000097:blood; D006801:Humans; D007106:Immune Sera; D013696:Temperature
D000682:Amyloid; D000941:Antigens; D000939:Epitopes; D007106:Immune Sera