The cytolytic T lymphocyte response to trinitrophenyl-modified syngeneic cells. I. Evidence for antigen-specific helper T cells.
Mice were primed subcutaneously with trinitrophenyl (TNP)-modified syngeneic spleen cells. Seven days later, spleen cells from these in vivo primed mice, or spleen cells from naive mice, were co-cultured with TNP-modified syngeneic cells. Spleen cells from the in vivo primed mice demonstrated augmented cytolytic T lymphocyte (CTL) activity. The spleens of these in vivo primed mice contained a population of radioresistant, antigen-specific, helper T cells. Specifically, spleen cells from these mice, after x-irradiation, were able to augment the in vitro CTL response of normal spleen cells to TNP-modified syngeneic cells.
D000818:Animals; D000961:Antilymphocyte Serum / Q000494:pharmacology; D003429:Cross Reactions; D003602:Cytotoxicity, Immunologic; D000939:Epitopes; D005260:Female; D006183:H-2 Antigens / Q000276:immunology; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D009578:Nitrobenzenes / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D014301:Trinitrobenzenes / Q000276:immunology*; D014965:X-Rays
D000961:Antilymphocyte Serum; D000939:Epitopes; D006183:H-2 Antigens; D009578:Nitrobenzenes; D014301:Trinitrobenzenes