The use of fluorescein conjugated antisera to identify proteins on stained and dried electrophoresis strips.
Paraprotein bands discovered on routine electrophoresis may be identified immunologically without repeating the electrophoretic separation. It is only necessary to heat the stained and dried strip in a hot air oven to reactivate the antigenic sites and insolubilise the protein. The strip may then be stained using specific antisera conjugated with fluorescein. The absence of prozone phenomena greatly helps in typing of free light chains.
D004586:Electrophoresis; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D006801:Humans; D007072:Immunoglobulin D; D007073:Immunoglobulin E; D008722:Methods; D010266:Paraproteins / Q000032:analysis* / Q000276:immunology; D013194:Staining and Labeling
D005452:Fluoresceins; D007072:Immunoglobulin D; D010266:Paraproteins; D007073:Immunoglobulin E