Light microscope analysis of meiotic prophase chromosomes by silver staining.
A method is described for the silver staining of the synaptonemal complex in surface-spread mammalian spermatocytes for light microscope examination. The method is quick, reliable, of broad applicability, and provides a means of making karyotype analysis at meiotic prophase. Many hundreds of suitable cells can be examined in an average preparation in a relatively short space of time. It has so far been applied only to mammalian spermatocytes, but could be used for karyotype analysis in oocytes of mammals and also applied to gonocytes of non-mammalian species.
D000818:Animals; D002875:Chromosomes / Q000648:ultrastructure*; D003584:Cytological Techniques; D006801:Humans; D008297:Male; D008540:Meiosis; D051379:Mice; D011418:Prophase; D012834:Silver; D013090:Spermatocytes / Q000166:cytology; D013194:Staining and Labeling
D012834:Silver