Osmium dependent argentaffin reaction in the pancreatic islet.
By applying at the ultrastructural level the silver methenamine impregnation method to glutaraldehyde-osmium fixed pancreatic tissue of Snell-Bagg mice it was found that two islet cell types show deposition of reduced silver on their secretory granules: B cells and another cell type. Histochemical analyses indicate that very likely the osmium dependent argentaffinity is due to S-S groups. After alkaline treatment the reactivity of B cells is lost, while that of the other cells remains unaffected. It is suggested that the third cell type stores the pancreatic somatostatin, an S-S containing polypeptide hormone.
D000818:Animals; D003553:Cystine / Q000032:analysis; D005976:Glutaral; D007515:Islets of Langerhans / Q000032:analysis / Q000187:drug effects / Q000648:ultrastructure*; D008709:Methenamine; D051379:Mice; D009992:Osmium; D010455:Peptides / Q000032:analysis; D012834:Silver; D013194:Staining and Labeling
D010455:Peptides; D009992:Osmium; D012834:Silver; D003553:Cystine; D008709:Methenamine; D005976:Glutaral