Distribution of the thy-1 antigen in cellular and subcellular fractions of adult mouse brain.
By using a cytotoxicity inhibition assay employing AKR anti-C3H thymocyte antiserum, we have determined the degree of expression of the thy-1 antigen in fractions of adult mouse brain. As expressed as cytotoxicity inhibitory capacity per mg protein with C3H whole brain arbitrarily assigned a value of 1.0, the following values were found: C3H cerebral cortex, 5.8; C3H cerebral cortex synaptosomes, 2.5: C3H whole brain myelin, 0.65; C3H cerebral cortex neurons, 0.16; and C3H cerebral cortex mitochondria 0.10. Neither C1300 neuroblastoma cells nor any AKR neural fraction had detectable levels of thy-1. The findings indicate that the thy-1 antigen is found mainly in mouse cerebral cortex and in synaptosomal fractions, whereas myelin fractions contain lower but perhaps significant amounts of thy-1. Cerebral cortex neurons, isolated by a method requiring a 90-min mild trypsinization at 37 degrees C, did not display significant amounts of the thy-1 antigen. These results lend themselves to further study in the area of differentiation and development of central nervous system components and in the area of central nervous system immunopathology.
D000818:Animals; D000918:Antibody Specificity; D001921:Brain / Q000276:immunology*; D001932:Brain Neoplasms / Q000276:immunology; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000276:immunology; D003601:Cytotoxicity Tests, Immunologic; D000939:Epitopes; D005260:Female; D007518:Isoantibodies; D007519:Isoantigens; D008297:Male; D051379:Mice; D008806:Mice, Inbred AKR; D008809:Mice, Inbred C3H; D008928:Mitochondria / Q000276:immunology; D009186:Myelin Sheath / Q000276:immunology; D009374:Neoplasms, Experimental / Q000276:immunology; D009447:Neuroblastoma / Q000276:immunology; D009474:Neurons / Q000276:immunology; D013574:Synaptosomes / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000939:Epitopes; D007518:Isoantibodies; D007519:Isoantigens