Detection of SV40 T antigen with labelled antibodies: radioimmunoassay and autoradiography.
SV40 T antigen, partially purified from SV80, a human cell line, has been used to develop a sensitive solid phase radioimmunoassay. In this assay SV40 T antigen was immobilized on microtiter plates, reacted with hamster anti-SV40 T antibody and subsequently with 14C-labelled anti-hamster IgG. The test detected approximately 20 ng of partially purified SV40 T antigen and is rapid and more convenient than the complement fixing reaction. An immunoautoradiographic method which permits the determination of the cellular localization of SV40 T antigen and which is possibly more sensitive than the indirect immunofluorescence method is also described.
D000888:Antibodies, Anti-Idiotypic / Q000032:analysis; D000914:Antibodies, Viral; D000956:Antigens, Viral / Q000032:analysis* / Q000302:isolation & purification; D001345:Autoradiography; D002250:Carbon Radioisotopes; D002460:Cell Line; D003168:Complement Fixation Tests; D004279:DNA, Viral / Q000032:analysis; D000939:Epitopes; D005455:Fluorescent Antibody Technique; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007553:Isotope Labeling; D011863:Radioimmunoassay; D013539:Simian virus 40 / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D000914:Antibodies, Viral; D000956:Antigens, Viral; D002250:Carbon Radioisotopes; D004279:DNA, Viral; D000939:Epitopes; D007074:Immunoglobulin G