Purification and characterization of gibbon ape leukemia virus DNA polymerase.
An RNA directed DNA polymerase was purified over 2500 fold from gibbon ape leukemia virus by successive column chromatography on Sephadex G100, DEAE cellulose, phosphocellulose and hydroxyapatite. The purified DNA polymerase has a molecular weight of 68 000, a pH optimum of 7.5, a Mn2+ optimum of 0.8 mM, and KCl optimum of 80 mM. The purified enzyme transcribes heteropolymeric regions of viral 60-70 S RNA isolated from avian myeloblastosis virus, Rauscher murine leukemia virus and simian sarcoma virus and it is inhibited by antiserum prepared against either gibbon ape leukemia virus or simian sarcoma virus DNA polymerases.
D000818:Animals; D003429:Cross Reactions; D003854:Deoxyribonucleotides / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006922:Hylobates; D008345:Manganese / Q000494:pharmacology; D008970:Molecular Weight; D011189:Potassium Chloride / Q000494:pharmacology; D012367:RNA, Viral / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D012190:Retroviridae / Q000201:enzymology*; D014158:Transcription, Genetic
D003854:Deoxyribonucleotides; D012367:RNA, Viral; D008345:Manganese; D011189:Potassium Chloride; D012194:RNA-Directed DNA Polymerase