[Method of combined detection of nerve fibers and blood vessels in nerve trunks].
A new method for investigation of neuro-vasal relationships in nerve conductors is proposed based on a combination of the fine injecting of their blood vessels and staining the nerve fibres. The vessels are injected with the chloroform emulsion of finely ground Paris blue (5-10 g per 100 g of solvent). Pieces of nerves 0,5 cm thick are fixed in 12% neutral formalin for 3 days, kept in a dark vessel in Weigert's mortant for 5 days, dehydrated and imbedded in paraffin. Thin slices 4-5 micronkm thick are stained in hematoxylin after N. N. Kulchitski, differentiated for 2-12 h in the mixture containing 1% solution of potassium ferricyanide and saturated solution of lithium carbonate (1:10) and after passing through alcohols of increasing concentration and xylene imbedded in balsam. In the preparations the fibres of different caliber are stained grey and the vessels are stained blue.
D000818:Animals; D001369:Axons; D001808:Blood Vessels / Q000033:anatomy & histology*; D008722:Methods; D010525:Peripheral Nerves / Q000033:anatomy & histology* / Q000098:blood supply; D011817:Rabbits; D051381:Rats; D013194:Staining and Labeling; D014650:Vasa Nervorum / Q000033:anatomy & histology*
Metod sochetannogo vyiavleniia nervnykh volokon i krovenosnykh sosudov v nervnykh provodnikakh.