The binding of 8-methoxypsoralen to nuclear DNA of UVA irradiated human fibroblasts in vitro.
Human fibroblasts were exposed to tritium-labelled 8-methoxypsoralen (8-MOP) and longwave ultraviolet (UVA) light in vitro. The proportions of photochemically bound 8-MOP in the nucleus and cytoplasm were studied by autoradiography. The results show that UVA-irradiation of the cells in the presence of 8-MOP gives rise to a significant dose-dependent binding of 8-MOP in the cell nucleus.
D000818:Animals; D001345:Autoradiography; D001665:Binding Sites; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004247:DNA / Q000528:radiation effects*; D005347:Fibroblasts / Q000378:metabolism / Q000528:radiation effects; D006168:Guinea Pigs; D006801:Humans; D008730:Methoxsalen / Q000378:metabolism* / Q000494:pharmacology; D010778:Photochemotherapy; D011565:Psoriasis / Q000188:drug therapy; D014466:Ultraviolet Rays
D004247:DNA; D008730:Methoxsalen