title: The demonstration of cell-associated immunity to viruses. In vitro lymphocyte responsiveness to Varicella-zoster antigen.
abstract: The demonstration of in vitro lymphocyte responsiveness to common pediatric viruses has previously been fraught with many technical and conceptual problems. Based upon our prior experience in demonstrating cell-associated immunity to mumps, rubella and measles viruses we illustrate our methodology and conceptual framework by documenting in vitro lymphocyte responsiveness to the Varicella-zoster virus, another ubiquitous virus of childhood. We discuss our approach to the problems of reactivity, specificity and reliability in the use of membrane-associated viral antigens.
mesh_terms: D000956:Antigens, Viral; D004306:Dose-Response Relationship, Immunologic; D000939:Epitopes; D014645:Herpesvirus 3, Human / Q000276:immunology*; D006689:Hodgkin Disease / Q000276:immunology; D006801:Humans; D007111:Immunity, Cellular; D008213:Lymphocyte Activation
chemical_list: D000956:Antigens, Viral; D000939:Epitopes