Immunoglobulin-like messenger RNA in a mouse T cell lymphoma.
RNA-driven complementary DNA (cDNA) hybridization experiments have been carried out in order to detect complementary sequences in RNA prepared from a mouse T cell lymphoma line (EL4). In conditions where efficient hybridization of L-chain cDNA with homologous P3 myeloma mRNA was observed, poor hybridization was observed with EL4 mRNA unless a low criterion of hybrid formation was employed (i.e. hydroxyapatite fractionation). The hybrid formed between EL4 mRNA and L-chain cDNA was found to melt about 5 degrees C below the homologous hybrid indicating that the sequence detected in EL4 mRNA is similar but not identical with the P3 mRNA sequence. However, a similar sequence was detectable in a Clambda-producing myeloma cell line but in this line the concentration of the sequence was found to be an order of magnitude lower than in EL4 cells.
D001483:Base Sequence; D004247:DNA; D006358:Hot Temperature; D007147:Immunoglobulin Light Chains; D007145:Immunoglobulin kappa-Chains; D008223:Lymphoma / Q000276:immunology* / Q000378:metabolism; D009374:Neoplasms, Experimental / Q000276:immunology / Q000378:metabolism; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides; D012333:RNA, Messenger / Q000276:immunology*; D012194:RNA-Directed DNA Polymerase; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D014158:Transcription, Genetic
D007147:Immunoglobulin Light Chains; D007145:Immunoglobulin kappa-Chains; D009841:Oligonucleotides; D012333:RNA, Messenger; D004247:DNA; D012194:RNA-Directed DNA Polymerase