In vitro sensitization of human T lymphocytes to hapten (TNP)-conjugated and non-treated autologous cells is restricted by self-HLA-D.
By co-culturing human T lymphocytes with TNP-treated autologous B lymphocytes and macrophages for 10 days in vitro, sensitization to TNP-treated autologous cells could be detected in a proliferative assay. By restimulation with different types of allogeneic cells and with cells from donors compatible or incompatible for the HLA-ABC or -D determinants, results were obtained suggesting that the TNP-specific response was restricted by the HLA-D but not the -ABC antigens of the autologous priming cells. Furthermore, our experiments demonstrate that T lymphocytes can also be primed against non-treated autologous cells in vitro and suggest that the HLA-D determinants may be involved also in this auto-sensitization.
D001402:B-Lymphocytes / Q000276:immunology; D002478:Cells, Cultured; D000939:Epitopes; D006680:HLA Antigens; D006241:Haptens; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects; D008264:Macrophages / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014301:Trinitrobenzenes / Q000494:pharmacology
D000939:Epitopes; D006680:HLA Antigens; D006241:Haptens; D014301:Trinitrobenzenes