title: Differential and staining of cobalt-and nickel-filled neurones using rubeanic acid.
abstract: A staining procedure is described with differentiates with distinct colours, cobalt and nickel ions introduced into neurones. Densely coloured precipitates are produced with these ions when rubeanic acid, a spot-test reagent, is added. Formation of the coloured complexes takes place within a few minutes, and may be followed under the dissecting microscope. This technique permits observation of the relative positions, within ganglia, of somata related to two emergent nerves following multiple axonal-backfilling. It also appears to be of value in tracing neurone branching after the intracellular injection of ions.
mesh_terms: D000818:Animals; D001369:Axons / Q000648:ultrastructure; D003035:Cobalt; D003116:Color; D007202:Indicators and Reagents; D009474:Neurons / Q000648:ultrastructure*; D009532:Nickel; D012908:Snails; D013194:Staining and Labeling / Q000379:methods*; D013854:Thioamides
chemical_list: D007202:Indicators and Reagents; D013854:Thioamides; D003035:Cobalt; D009532:Nickel; C005728:rubeanic acid