Effects of thrombin, chymotrypsin and aggregated gamma-globulins on the proteins of the human platelet membrane.
Analysis of platelet membrane proteins and glycoproteins by SDS polyacrylamide gel electrophoresis was carried out before and after treatment with thrombin. Extended incubation with thrombin  (in the presence of EDTA or adenosine, which inhibit aggregation) produced extensive changes in the bands observed. With incubation times of a few minutes however, the changes were restricted to a glycopeptide, GP IV (approx. 90,000 Daltons) and one or two polypeptides of low molecular weight, in particular polypeptide 16 (approx. 23,000 Daltons). At 0--3 degrees C only polypeptide 16 was still hydrolyzed. Chymotrypsin, which does not activate platelets, attacked glycopeptides I, II, III but no changes were apparent in GP IV and polypeptide 16. When chymotrypsin-treated platelets were further incubated with thrombin, only GP IV and one to two low molecular weight polypeptides, especially polypeptide 16, were affected. As polypeptide 16 appears to be an integral membrane component it is possible that it, either by itself or in combination with GP IV, represents the primary thrombin substrate involved in platelet activation. Aggregated IgG, which also activates platelets, does not modify the membrane glycoproteins but does change the low molecular weight region in particular band 16.
D000241:Adenosine; D001792:Blood Platelets / Q000187:drug effects*; D002918:Chymotrypsin / Q000494:pharmacology*; D004492:Edetic Acid; D006020:Glycopeptides / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis; D006801:Humans; D007074:Immunoglobulin G / Q000494:pharmacology; D008564:Membrane Potentials; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis; D013917:Thrombin / Q000494:pharmacology*; D013997:Time Factors; D005719:gamma-Globulins / Q000494:pharmacology*
D006020:Glycopeptides; D006023:Glycoproteins; D007074:Immunoglobulin G; D010455:Peptides; D005719:gamma-Globulins; D004492:Edetic Acid; D002918:Chymotrypsin; D013917:Thrombin; D000241:Adenosine