Detection of intermediate complexes by evaluation of the difference between gamma-globulin and IgG concentrations.
A simple analysis based on techniques readily available in the routine clinical laboratory has been presented for identification of patients with moderate and high levels of intermediate complexes. If the gamma-globulin concentration determined by serum protein electrophoresis minus the IgG concentration determined by radial immunodiffusion exceeds 1.0 gm/dl, intermediate complexes of IgG should be present in concentrations greater than 0.5 gm/dl. In principle, any disease association with sufficient polymerization of IgG might cause an abnormal result with this analysis. Furthermore, sera from patients with liver disease and marked beta-gamma-bridging on electrophoresis may also give abnormal values.
D001172:Arthritis, Rheumatoid / Q000097:blood; D001782:Blood Donors; D001797:Blood Protein Electrophoresis; D004588:Electrophoresis, Cellulose Acetate; D005069:Evaluation Studies as Topic; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis*; D008722:Methods; D011693:Purpura / Q000097:blood; D012217:Rheumatoid Factor / Q000032:analysis*; D005719:gamma-Globulins / Q000032:analysis*
D007074:Immunoglobulin G; D005719:gamma-Globulins; D012217:Rheumatoid Factor