Secretion rate independent evaluation of IgM antibody avidity at the level of single immunocytes.
The hemolytic plaque inhibition assay has been performed on spleen cells from mice immunized with TNP-HRBC to evaluate avidity of anti-TNP IgM antibodies. At different times after immunization direct plaques were inhibited by soluble TNP-EACA, TNP61-BGG, or anti-mu antiserum. Analysis of the inhibition data provided independent estimates of antibody avidity and secretion rate. Avidity was found to increase with time, to reach a maximum when the antibody response attained the peak value, and then to decline as the response was waning. There was a decrease followed by increase of the secretion rate concomitant with the rise and fall of the antibody response and avidity.
D015119:Aminocaproic Acid / Q000276:immunology; D000818:Animals; D000915:Antibody Affinity; D002417:Cattle; D005260:Female; D006462:Hemolytic Plaque Technique; D007075:Immunoglobulin M; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D011817:Rabbits; D013154:Spleen / Q000276:immunology*; D014301:Trinitrobenzenes / Q000276:immunology; D005719:gamma-Globulins / Q000276:immunology
D007075:Immunoglobulin M; D014301:Trinitrobenzenes; D005719:gamma-Globulins; D015119:Aminocaproic Acid