Synthetic peptides comprising sequences of the human immunoglobulin E heavy chain capable of releasing histamine.
On the basis of previous studies on the structure-activity relationship of model polypeptide histamine liberators, a site within the Fc region of immunoglobulin E antibody molecules has been proposed as that responsible for the direct triggering of target mast cells after antigen challenge. Peptides comprising this region of the epsilon-chain have now been synthesized and shown to induce histamine release from normal rat peritoneal mast cells in a selective manner essentially similar to that mediated by anaphylactic antibody-antigen interaction.
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D004305:Dose-Response Relationship, Drug; D006636:Histamine Release / Q000187:drug effects*; D007073:Immunoglobulin E; D007143:Immunoglobulin Heavy Chains; D066298:In Vitro Techniques; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000378:metabolism; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology / Q000494:pharmacology*; D051381:Rats
D007143:Immunoglobulin Heavy Chains; D010455:Peptides; D007073:Immunoglobulin E