Comparative studies of the incorporation inhibition of radioactive nucleotides and amino acids in vitro under the influence of adriamycin, bleomycin, and sodium arsenate.
The incorporation of 14C-marked nucleotides and amino acids into the nucleic acids and into the cellular protein of PHA-stimulated human lymphocytes was inhibited variably by increasing doses of Adriamycin, Bleomycin and Na2HAsO4. The findings, which were obtained with the aid of the liquid scintillation counter and partly by means of 14C-thymidine-marked autoradiographies shows clearly that Adriamycin causes the strongest incorporation inhibition. In contrast to this, the inhibition caused by Bleomycin and arsenate is considerably less destructive. It was shown by means of cytophoto metrical examinations that Adriamycin is capable of fixing cells in the G2 phase and in the S phase. Cells react similarly to Bleomycin and arsenate. The differential inhibition of nucleic acid and protein metabolism induced by Adriamycin, Bleomycin and arsenate is correlated with a varying responsiveness of lymphocytes to the cytostatically effective substances.
D001149:Arsenates / Q000494:pharmacology*; D001151:Arsenic / Q000494:pharmacology*; D001761:Bleomycin / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis; D004317:Doxorubicin / Q000494:pharmacology*; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism; D008938:Mitosis / Q000187:drug effects; D009696:Nucleic Acids / Q000096:biosynthesis*; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis
D001149:Arsenates; D009696:Nucleic Acids; D001761:Bleomycin; D012313:RNA; D004317:Doxorubicin; D004247:DNA; D001151:Arsenic