Reverse transcriptase from avian myeloblastosis virus.
From lots of 20 to 30 g of avian myeloblastosis virus RNA-dependent DNA polymerase was obtained in preparations of purity greater than 95% by using a two-step column chromatographic procedure employing DEAE (DE 52) and carboxymethylcellulose (CM 52.). Yields of RNA-dependent DNA polymerase varied from approximately 20,000 to 35,000 U/g of virus. Specific activity of the enzyme was about 35,000 to 60,000 U/mg of protein. Free of detectable RNase activity, the product exhibited a molecular weight of about 160,000, an isoelectric point of 6.5, and approximately 2 mol of fatty acid per mol of enzyme.
D000818:Animals; D001353:Avian Leukosis / Q000382:microbiology*; D001354:Avian Leukosis Virus / Q000201:enzymology*; D009189:Avian Myeloblastosis Virus / Q000201:enzymology*; D001769:Blood / Q000382:microbiology; D002645:Chickens; D005227:Fatty Acids / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis; D007526:Isoelectric Point; D008970:Molecular Weight; D012194:RNA-Directed DNA Polymerase / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism; D014764:Viral Proteins / Q000032:analysis
D005227:Fatty Acids; D006023:Glycoproteins; D014764:Viral Proteins; D012194:RNA-Directed DNA Polymerase