Studies with B-cell allo- and hetero-antisera: parallel reactivity and special properties.
The expression of B-cell antigens on various cell populations was studied through the use of human alloantisera and with heteroantisera raised to preparations of the alloantigen bearing molecules isolated from B-cell lines. The allo-and hetero-antisera competed with each other in blocking experiments and gave closely parallel results, reacting with normal and leukemic B lymphocytes, monocytes, E-rosette-negative acute lymphatic leukemias, all acute and certain chronic myelogenous leukemias, and a minor population of cells in fetal spleen and liver. These highly immunogenic surface components appeared to comprise the dominant B- cell specific plasma membrane determinants. Neither type of antiserum reacted with any but a minor population of normal or pokeweed-mitogen-transformed T cells, fetal thymic lymphocytes, E-rosette-positive acute lymphatic leukemias, or Sezary-cell leukemia. Through the use of these antisera evidence was obtained that Fc-receptor-bearing Ig-negative lymphocytes were divisible into two groups according to the presence or absence of the B-cell antigens. Both hetero- and allo-antisera blocked binding of immune complexes or antibody-coated ox erythrocytes to Fc receptors on B cells. F(ab')2 fragments of the heteroantibodies strongly inhibited antibody-dependent cell-mediated killing.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D015478:Antigens, Heterophile / Q000032:analysis*; D001402:B-Lymphocytes / Q000276:immunology*; D001667:Binding, Competitive; D002458:Cell Fractionation; D002469:Cell Separation; D003601:Cytotoxicity Tests, Immunologic; D000939:Epitopes; D005333:Fetus; D005455:Fluorescent Antibody Technique; D006801:Humans; D007106:Immune Sera; D007111:Immunity, Cellular; D007114:Immunization; D007140:Immunoglobulin Fab Fragments / Q000032:analysis; D007141:Immunoglobulin Fc Fragments / Q000032:analysis; D007519:Isoantigens / Q000032:analysis*; D007938:Leukemia / Q000276:immunology; D008213:Lymphocyte Activation; D008934:Mitogens / Q000494:pharmacology; D009000:Monocytes / Q000276:immunology; D011817:Rabbits
D000906:Antibodies; D015478:Antigens, Heterophile; D000939:Epitopes; D007106:Immune Sera; D007140:Immunoglobulin Fab Fragments; D007141:Immunoglobulin Fc Fragments; D007519:Isoantigens; D008934:Mitogens