[Experiments for the serological establishment of the species of mycobacteria inducing an immune restructuring of the macroorganism].
Serological investigations are carried out on hyperimmune rabbit and guinea pig sera with two antigens each of Mycobacterium tuberculosis H37Rv, Mycobacterium bovinus, Mycobacterium avium and Mycobacterium Kanzassi. One of the antigens is obtained through electrohydraulic destruction of delipidated mycobacteria, while the other constitutes cell walls, purified with ribonuclease, of non-delipidated mycobacterial cells, destroyed by the same method. The antigens, prepared by the electrohydraulic method, possess a markedly expressed complement-fixing capacity, conversely to the antigen from cell walls, which is slightly active in complement-fixation tests. Again a trend is observed towards a possible serological indentification of the species of mycobacteria that have caused the immune transformation of the major organism; yet the questions still remain to be settled. The immunogenes used possess a high activity and induce high titre antisera, established also in other investigations, which make reasonable their study as vaccines.
D000042:Absorption; D000818:Animals; D000937:Antigen-Antibody Reactions; D000942:Antigens, Bacterial / Q000032:analysis; D002473:Cell Wall / Q000276:immunology; D003168:Complement Fixation Tests; D000939:Epitopes; D006168:Guinea Pigs; D007106:Immune Sera / Q000032:analysis; D009161:Mycobacterium / Q000276:immunology*; D009162:Mycobacterium avium / Q000276:immunology; D009163:Mycobacterium bovis / Q000276:immunology; D009169:Mycobacterium tuberculosis / Q000276:immunology; D011817:Rabbits; D012703:Serotyping; D013045:Species Specificity
D000942:Antigens, Bacterial; D000939:Epitopes; D007106:Immune Sera
Opiti za serologichno ustanoviavane na vida mikobakterii, predizvikali imunna prenaglasa na makroorganizma.