Purified antigen radioimmunoassay in serological diagnosis of schistosomiasis mansoni.
A radioimmunoassay has been developed for detecting human schistosomiasis with a highly purified Schistosoma mansoni egg antigen labelled with iodine-125. The antigen, major serologic antigen 1 (M.S.A.1), has striking immunochemical and species specificity and appears to be the principal antigen responsible for the granulomatous response to S. mansoni eggs. This 125I-antigen was tested with 5 microliter of patient serum as a potential serodiagnostic test for schistosomiasis. 92 control sera from uninfected St. Vincentian patients were seronegative. In studies on 135 lightly infected St. Lucians the 5 microliter serum 125I-M.S.A.1 serodiagnostic test was more sensitive than any tests available at the Center for Disease Control, with 64% of infected children, 83% of adolescents, and 98% of adults being positive. 49 heavily infected Kenyans of all age-groups were uniformly (100%) seropositive. Finally, a semiquantitative version of the 125I-M.S.A.1 radioimmunoassay that uses 0-5 microliter of patient sera demonstrated quantitatively significant differences among age-watched and intensity-watched groups of patients with S. mansoni, S. haematobium, and S. japonicum infections.
D000293:Adolescent; D000328:Adult; D000906:Antibodies / Q000302:isolation & purification; D000941:Antigens / Q000302:isolation & purification; D001666:Binding Sites, Antibody; D002648:Child; D002675:Child, Preschool; D003168:Complement Fixation Tests; D005069:Evaluation Studies as Topic; D005260:Female; D005428:Flocculation Tests; D005455:Fluorescent Antibody Technique; D006801:Humans; D007457:Iodine Radioisotopes; D007553:Isotope Labeling; D007630:Kenya; D009820:Ohio; D010063:Ovum / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods*; D012547:Schistosoma; D012548:Schistosoma haematobium; D012550:Schistosoma mansoni / Q000276:immunology; D012552:Schistosomiasis / Q000175:diagnosis* / Q000469:parasitology; D013045:Species Specificity
D000906:Antibodies; D000941:Antigens; D007457:Iodine Radioisotopes