Cytological analyses of 14p+ variant by means of N-banding and combinations of silver staining and chromosome bandings.
An inherited human karyological variant (14p+) has been studied with a number of cytochemical techniques. The short arm of this variant chromosome 14 is nearly as long as the long arm, giving the chromosome a submetacentric to metacentric appearance. In conventionally Giemsa-stained preparations, maximum of three secondary constrictions can be observed the marker arm. The secondary constrictions are silver-positive in Ag-NOR preparations. However, the entire arm stains deeply in N-banded preparations. The 14p+ arm is also Q-negative, C-negative, G-negative, and R-positive with an almost homogeneous texture. The difference between N-banding and silver staining is interpreted as the result of gene activities of the ribosomal cistrons.
D001399:Azure Stains; D002466:Cell Nucleolus; D002871:Chromosome Banding; D002901:Chromosomes, Human, 13-15 / Q000648:ultrastructure*; D005260:Female; D014644:Genetic Variation; D006801:Humans; D012834:Silver; D013194:Staining and Labeling
D001399:Azure Stains; D012834:Silver