Immunocytochemical localization of plasminogen activator on porcine kidney cell strain: LLC-PK1 (LP100).
An antibody to plasminogen activator (PA) produced by the cultured cells of the pig kidney cell strain LLC-PK1 (LP100) was used to localize PA on the cell's free (unattached) surface. Localization was accomplished by the unlabeled antibody enzyme method (PAP) at the light microscopic level and at the electron microsopic level. Localization was commonly more intense at cell to cell junctions and was associated with blebs and vesiculation in this area. We are proposing that membrane shedding by blebs and vesiculation may be the mechanism of PA release in the LLC-PK1 (LP100) cell strain.
D000818:Animals; D002460:Cell Line; D005976:Glutaral; D007668:Kidney / Q000032:analysis*; D008854:Microscopy, Electron; D010960:Plasminogen Activators / Q000032:analysis* / Q000276:immunology; D013194:Staining and Labeling; D013552:Swine
D010960:Plasminogen Activators; D005976:Glutaral