B cell tolerance. I. Analysis of hapten-specific unresponsiveness induced in vitro in adult and neonatal murine spleen cell populations.
The cellular mechanisms and tolerogen dose requirements of hapten-specific unresponsiveness induced in vitro by using 2,4,6-trinitrophenyl human gamma-globulin (TNP17HgG) were analyzed in adult and neonatal murine splenocytes. Tolerance induction in both cell populations was found to be independent of non-B cell effects including BAtheta-positive cells, Ly 2.2-positive cells, adding or reducing the number of macrophages, and large excesses of HgG. The tolerance induced was specific and not "infectious", further excluding a role for suppressor T cells. Neonatal splenic B cells were rendered tolerant by doses of TNP17HgG 1000-fold less than those required to produce similar tolerance in splenic B cells from adults. These findings support the concept of functional clonal abortion as a mechanism for producing tolerance to self antigens.
D000375:Aging; D000818:Animals; D000831:Animals, Newborn; D000961:Antilymphocyte Serum / Q000494:pharmacology; D001402:B-Lymphocytes / Q000276:immunology*; D003165:Complement System Proteins; D004306:Dose-Response Relationship, Immunologic; D000939:Epitopes; D006241:Haptens; D006462:Hemolytic Plaque Technique; D006801:Humans; D007108:Immune Tolerance; D007111:Immunity, Cellular; D007165:Immunosuppression Therapy; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D013154:Spleen / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D014301:Trinitrobenzenes / Q000276:immunology; D005719:gamma-Globulins / Q000276:immunology
D000961:Antilymphocyte Serum; D000939:Epitopes; D006241:Haptens; D014301:Trinitrobenzenes; D005719:gamma-Globulins; D003165:Complement System Proteins