Presence of a high-molecular-weight RNA and RNA-directed DNA polymerase in rabbit hereditary lymphosarcoma.
Rabbit lymphosarcoma tissues contain 70 S RNA and RNA-directed DNA polymerase encapsulated in particulate components that band in the density region of type C RNA viruses. RNA-directed DNA polymerase associated with the particles could be distinguished from cellular DNA polymerases by salt elution from phosphocellulose. The enzyme preferred the template primers poly(rA)-(dT)12-18 and poly(rC)-(dG)12-18 over other synthetic template primers and also utilized viral 70 S RNA as template; these properties are not observed with the known cellular DNA polymerases.
D000818:Animals; D002478:Cells, Cultured; D005260:Female; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics* / Q000378:metabolism* / Q000382:microbiology; D008297:Male; D008970:Molecular Weight; D011119:Polynucleotides / Q000378:metabolism; D012334:RNA, Neoplasm / Q000302:isolation & purification*; D012367:RNA, Viral / Q000302:isolation & purification*; D012194:RNA-Directed DNA Polymerase / Q000302:isolation & purification*; D011817:Rabbits; D012190:Retroviridae / Q000201:enzymology; D012513:Sarcoma, Experimental / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology
D011119:Polynucleotides; D012334:RNA, Neoplasm; D012367:RNA, Viral; D012194:RNA-Directed DNA Polymerase