[Volumetric and cytologic investigations of cytolysis of ascites tumor cells provoked by triton X-100 (author's transl)].
Concentration-effectivity curves of Triton X-100 activity on ascitic cells of mouse leukemia 1210 and Zajdela-Hepatoma in rats have been plotted by electronic determination of cell numbers, indicating only one part of cytolysis which follows a sudden decrease of cell volumes. A better characterization of the whole cytolysis can be realised by volumetricall particle counter analysis. It first displays an increase in cell volumes for several concentrations in connection with microscopically observed increase in cells staining relatively weak by eosin. Staining and swelling nearly simultaneosly reach their maximum. Furthermore a sudden diminution of the cells follows with a shift of cell volumes distribution curves to the left, determined by particle counter depending on temperature, e. g. a strong delay at 0 degrees C.
D000818:Animals; D002452:Cell Count; D002478:Cells, Cultured; D007942:Leukemia, Experimental / Q000473:pathology*; D051379:Mice; D009368:Neoplasm Transplantation; D011092:Polyethylene Glycols / Q000494:pharmacology*; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology*; D013194:Staining and Labeling; D014184:Transplantation, Homologous
D000644:Quaternary Ammonium Compounds; D011092:Polyethylene Glycols
Verlauf der Zytolyse von Aszites-Tumorzellen durch Triton X-100 (Volumetrische und zytologische Untersuchungen)