[Source of valine for protein V biosynthesis in a producer of actinomycin C].
The specific activity of 14C-valine in valyl-tRNA formed during incubation of the actinomycin C-producing organism with 14C-valine was constant and lower than that of the whole cell pool. The constancy of the valyl-tRNA was indicative of the presence of a separate compartment for the valine pool used for protein biosynthesis. A lower specific activity of valine in valyl-tRNA as compared to that of the whole cell pool may be indicative of a low rate of valine metabolism in such separate compartment with exogenic 14-valine or a higher concentration of free valine in it as compared to the specific activity of this amino acid at average per cell.
D000190:Actinomyces / Q000378:metabolism*; D000596:Amino Acids / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis*; D003609:Dactinomycin / Q000096:biosynthesis*; D005609:Free Radicals; D006868:Hydrolysis; D012343:RNA, Transfer / Q000378:metabolism; D014633:Valine / Q000378:metabolism*
D000596:Amino Acids; D001426:Bacterial Proteins; D005609:Free Radicals; D003609:Dactinomycin; D012343:RNA, Transfer; D014633:Valine
Istochnik valina dlia biosinteza belka v produtsente aktinomitsina C.