Purification of rabbit T-derived (Ig-) lymphocytes.
Rabbit peripheral blood cells were rosetted by means of mixed agglutination technique described by Coombs et al. and the unrosetted Ig-, T-derived lymphocytes were separated in Ficoll-Triosil gradient. The cells which did not rosette in mixed agglutination reaction represented highly purified T-derived (Ig-) lymphocytes, as evidence by their response to T and B rabbit cell mitogens, their cytotoxic reactivity versus rabbit thymus lymphocyte antiserum (RTLA), and by failure to be stained with labelled anti-rabbit immunoglobulins antibodies and antibodies against allotypic specificities of the a and b series.
D000818:Animals; D000961:Antilymphocyte Serum / Q000494:pharmacology; D002469:Cell Separation; D003601:Cytotoxicity Tests, Immunologic; D007140:Immunoglobulin Fab Fragments / Q000032:analysis; D007136:Immunoglobulins / Q000032:analysis*; D008213:Lymphocyte Activation; D010544:Peroxidases / Q000378:metabolism; D011817:Rabbits; D013194:Staining and Labeling; D013601:T-Lymphocytes / Q000276:immunology*
D000961:Antilymphocyte Serum; D007140:Immunoglobulin Fab Fragments; D007136:Immunoglobulins; D010544:Peroxidases