A radioimmunoassay for bleomycin.
A radioimmunoassay for bleomycin, suitable for monitoring serum and urine drug levels, has been developed. Antisera were obtained from rabbits immunized with a conjugate of bleomycin and human serum albumin.Bleomycin labeled with 57Co was used as the tracer. Antibody-bound and free bleomycin were separated by precipitation with polyethylene glycol. The assay could determine bleomycin levels down to 0.025 mug/ml. The assay was precise, with a coefficient of variation of 2.8% Other drugs likely to be administered in combination with bleomycin did not interfere with the radioimmunoassay.
D000818:Animals; D000906:Antibodies; D001761:Bleomycin / Q000097:blood / Q000378:metabolism* / Q000652:urine; D003037:Cobalt Radioisotopes; D006801:Humans; D007457:Iodine Radioisotopes; D011092:Polyethylene Glycols; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods*
D000906:Antibodies; D003037:Cobalt Radioisotopes; D007457:Iodine Radioisotopes; D001761:Bleomycin; D011092:Polyethylene Glycols