Immunochemical analysis of soluble antigens of Mycobacterium lepraemurium.
Soluble antigens obtained from purified M. lepraemurium were identified by SDS polyacrylamide gel electrophoresis as three different proteins, M-W 1 X 10(4), 1 X 10(5), and 4 X 10(6), and two polysaccharide-polypeptides. Three antigens were found to react with anti-M. leprae antiserum; a single antigen proved to be immunologically related to M. tuberculosis, M. similae, M. balnei and a few other mycobacteria, but not to Eubacteriales and fungi, as examined by immunoelectrophoresis. In the dermal hypersensitivity assay, the guinea pigs sensitized with soluble antigens of M. lepraemurium only responded to the antigens of M. lepraemurium, M. leprae and partly to M. scrofulaceum, but not to other antigens, including lepromin and leprous serum globulins.
D000192:Actinomycetales / Q000276:immunology; D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis*; D001419:Bacteria / Q000276:immunology; D003429:Cross Reactions; D000939:Epitopes; D005658:Fungi / Q000276:immunology; D006168:Guinea Pigs; D006968:Hypersensitivity, Delayed; D009167:Mycobacterium lepraemurium / Q000276:immunology*; D010455:Peptides / Q000032:analysis; D011135:Polysaccharides, Bacterial / Q000032:analysis; D012995:Solubility
D000942:Antigens, Bacterial; D000939:Epitopes; D010455:Peptides; D011135:Polysaccharides, Bacterial