title: Histochemical demonstration of enzyme activities in plastic and paraffin embedded tissue sections.
abstract: Histochemical staining for enzymes is usually performed on frozen sections. This report lists the longer incubation times required to demonstrate esterase, acid phosphatase, beta-galactosidase, and cytochrome oxidase in plastic embedded and ruotine paraffin embedded tissues. The sections embedded in plastic, i.e. water soluble methacrylate (Polyscience's JB-4) and cut at 2 micrometers, were far superior to frozen sections and paraffin embedded sections both in tissue detail and in the localization of the histochemical reaction product.
mesh_terms: D000135:Acid Phosphatase / Q000032:analysis*; D000818:Animals; D003576:Electron Transport Complex IV / Q000032:analysis*; D004950:Esterases / Q000032:analysis*; D005696:Galactosidases / Q000032:analysis*; D006651:Histocytochemistry; D008264:Macrophages / Q000201:enzymology*; D009163:Mycobacterium bovis; D010232:Paraffin; D010969:Plastics; D011817:Rabbits; D013194:Staining and Labeling; D001616:beta-Galactosidase / Q000032:analysis*
chemical_list: D010969:Plastics; D010232:Paraffin; D003576:Electron Transport Complex IV; D004950:Esterases; D000135:Acid Phosphatase; D005696:Galactosidases; D001616:beta-Galactosidase