Reaction of tRNAPhe from yeast with 1-fluoro-2,4-dinitrobenzene. Attachment sites of the potential antigenic-determining 2,4-dinitrophenyl residues.
The reaction of 1-fluoro-2,4-dinitrobenzene with tRNAPhe from yeast, for the introduction of antigenic-determining 2,4-dinitrophenyl residues into tRNA, took place only at adenosine residues in tRNAPhe. After reaction at pH 8.0 and 50 degrees C two kinds of products were detected: one was ribose-modified adenosine which was derived from the 3' terminus of tRNA, and the other was base-modified adenosine. The sites and extent of the modification of each particular adenosine residue of tRNAPhe were determined as follows: 5 (6% modified), 31 (2%), 35 (36%), 67 (5%), and 76 (51%). Thus mainly the terminal adenosine and one adenosine in the anticodon loop bear the 2,4-dinitrophenyl residue.
D001483:Base Sequence; D055598:Chemical Phenomena; D002621:Chemistry; D004139:Dinitrofluorobenzene; D004140:Dinitrophenols; D000939:Epitopes; D007700:Kinetics; D009578:Nitrobenzenes; D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides / Q000032:analysis; D010649:Phenylalanine; D012343:RNA, Transfer; D012265:Ribonucleotides / Q000032:analysis; D012441:Saccharomyces cerevisiae; D013056:Spectrophotometry, Ultraviolet
D004140:Dinitrophenols; D000939:Epitopes; D009578:Nitrobenzenes; D009843:Oligoribonucleotides; D012265:Ribonucleotides; D010649:Phenylalanine; D012343:RNA, Transfer; D004139:Dinitrofluorobenzene