[Several structural characteristics of vital dyes].
Lymphocytes and macrophages of normal rats, cells of lymphosarcoma and ovarian carcinoma of rats (CC strain), cells of sarcoma 37 and lymphosarcoma Nk/Ly of mice were investigated for their capacity for vital staining by 86 days in concentrations within the range of 10(-5)--3-10(-2) M. It was found that alkylation of amino groups of the basic dyes increased their capacity for penetrating the living cells and depositing in their cytoplasm in cases of thiazine, oxazine, acridine, xanthene, diazine, and triphenylmethane compounds. The acid radicals decreased the capacity of the dye to vital staining of the cells considerably. The degree of basicity of the dye molecule and its amino groups played no decisive role in the process of vital staining.
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D008214:Lymphocytes / Q000166:cytology; D008264:Macrophages / Q000166:cytology; D051379:Mice; D009374:Neoplasms, Experimental / Q000473:pathology*; D051381:Rats; D013194:Staining and Labeling
Nekotorye strukturnye osobennosti vital'nykh krasiteleĭ