Cross-reactions of HLA antibodies: VII. Rate of sensitization and serological specificity.
Varying the sensitization time of the complement-dependent antibody mediated cytotoxicity test, while carefully controlling other test conditions, has enabled us to characterize the subtle reactivities of several broadly reactive sera. In particular, we can discriminate between classes of cells, e.g., HLA-B7 from B27; subclasses cells, e.g., Bw51 from Bw52; and cells carrying new antigens, e.g., Ao79. This manipulation of the cytotoxicity test is discussed in view of its usefulness in separating primary from cross-reactive specificities and in detecting new specificities.
D000328:Adult; D000920:Antibody-Dependent Cell Cytotoxicity; D003429:Cross Reactions; D003601:Cytotoxicity Tests, Immunologic; D000939:Epitopes; D006680:HLA Antigens / Q000276:immunology*; D006801:Humans; D007106:Immune Sera; D007114:Immunization; D007518:Isoantibodies; D008214:Lymphocytes / Q000276:immunology
D000939:Epitopes; D006680:HLA Antigens; D007106:Immune Sera; D007518:Isoantibodies