Serological analysis of antigen-specific helper factors specific for poly-L(Tyr, Glu)-poly-DLAla--poly-LLys [(T, G)-A--L] and L Glu60-LAla30-LTyr10 (GAT).
In vitro prepared antigen-specific helper factors reactive to the synthetic polypeptide antigens poly-L(Tyr, Glu)-poly-DLAla--poly-LLys [(T, G)-A--L] or LGlu60-LAla30-LTyr10 (GAT) and bearing Ia determinants were analyzed serologically to determine the nature of the Ia determinants they expressed. I subregion-specific mouse anti-Ia antisera were used, and showed that (T, G)-A--L-specific helper factor (HF) contains I-A subregion-controlled determinants, whereas GAT-specific HF carries I-J subregion-controlled antigens. This unexptected finding was confirmed in both the H-2k and H-2 b haplotypes, using a variety of anti-I-J antisera. Rabbit anti-Ia antisera also reacted with both HF which raised the possibility that the Ia determinants on HF may be carbohydrate in nature. The fact that HF has a low molecular weight and yet contains Ia determinants, antigen-binding capacity and idiotypic markers is compatible with this interpretation.
D000327:Adsorption; D000818:Animals; D000939:Epitopes; D004912:Erythrocytes / Q000276:immunology; D005802:Genes, MHC Class II; D006649:Histocompatibility Antigens; D007106:Immune Sera / Q000494:pharmacology; D007164:Immunosorbents; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D010455:Peptides / Q000276:immunology*; D011108:Polymers; D011817:Rabbits; D012756:Sheep; D013601:T-Lymphocytes / Q000276:immunology
D000939:Epitopes; D006649:Histocompatibility Antigens; D007106:Immune Sera; D007164:Immunosorbents; D010455:Peptides; D011108:Polymers; C020749:GAT