Immunohistochemistry on Bouin-fixed fetal tissue, stored for thirty years in ethanol.
Human fetal tissue fixed with Bouin and kept for 30 years in ethanol is still useful in immunohistochemistry. The localization of beta-endorphin immunoreactive cells in the pituitary is given as example.
D004723:Endorphins / Q000032:analysis*; D000431:Ethanol; D005260:Female; D005333:Fetus / Q000166:cytology*; D006651:Histocytochemistry; D006801:Humans; D007202:Indicators and Reagents; D010903:Pituitary Gland, Anterior / Q000032:analysis* / Q000166:cytology; D011247:Pregnancy; D013194:Staining and Labeling; D013997:Time Factors
D004723:Endorphins; D007202:Indicators and Reagents; D000431:Ethanol