Standardization of Romanowsky stains. The relationship between stain composition and performance.
A panel of 17 eminent haematologists has assessed the performance of 5 Romanowsky stains prepared from pure component dyes, comparing the suitability and acceptability of these stains for the preparation of routine blood and bone-marrow films. It was found that the results obtained using the stain described by Marshall et al (1975) were comparable to those obtained using a modification of the stain described by Wittekind et al (1976). The performance of the 3 other stains was less acceptable. Variations in stain formulation have been correlated with stain performance.
D001773:Blood Cells; D001854:Bone Marrow Cells; D006801:Humans; D013194:Staining and Labeling / Q000592:standards*; D013329:Structure-Activity Relationship