Micro-ELISA for detecting and assaying snake venom and venom-antibody.
Enzyme-linked immunosorbent assay (ELISA) can detect venom levels down to 1-5 ng/ml. The assay is specific; no cross-reaction of clinical importance was found in a range of 14 different types of venom. Both venom and venom-antibody levels were monitored in mice. Specific antibody was detected in human serum more than 2 years after an accidental bite by Echis carinatus. ELISA should clarify many basic problems of envenoming in man.
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D006801:Humans; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D008722:Methods; D051379:Mice; D011817:Rabbits; D012910:Snake Venoms / Q000276:immunology / Q000302:isolation & purification*; D013053:Spectrophotometry
D000906:Antibodies; D007106:Immune Sera; D012910:Snake Venoms