title: [Concentration of free 17-beta estradiol in the plasm of ewes following treatment with chlormadione acetate (CAP) in 2 synchronized cycles].
abstract: Six ewes of the Merino breed were subjected to radioimmunological assay (RIA) for the study of free 17-beta estradiol during the instillation of vaginal tampons soaked with 30 mg chlormadinone acetate (CAP), and 17 and 34 days after removal of the tampons. On the day before the administration of the preparation, the concentrations of free 17-beta estradiol were comparatively low, ranging within the limits of 38.6 +/- 5.6 pg ml-1 of plasma. These values showed no significant changes during the instillation of the tampons. When the tampons were removed, three periods of increased 17-beta estradiol levels were recorded: 1. on the 1st to 3rd day after removal of the tampons with a peak of 93.2 +/- 26.7 pg ml-1, 2. on the 13th to 16th day with the highest value of 59.7 +/- 23.6 pg ml-1, 3. on the 30th to 33rd day after removal of the tampons 76.2 +/- 23.5 pg ml-1. The results showed that the endocrine activity of the ovaries, expressed by a change in the concentration of plasma 17-beta estradiol after removal of the tampons (CAP), repeated in similar time intervals as under natural conditions and was probably associated with repeated growth and ripening of the follicles of ovaries and their ovulation.
mesh_terms: D000818:Animals; D002715:Chlormadinone Acetate / Q000008:administration & dosage*; D004958:Estradiol / Q000097:blood*; D004973:Estrus Synchronization / Q000187:drug effects*; D005260:Female; D011247:Pregnancy; D011863:Radioimmunoassay; D012756:Sheep / Q000097:blood
chemical_list: D002715:Chlormadinone Acetate; D004958:Estradiol
vernacular_title: Koncentrácia vol'ného 17-beta estradiolu v krvnej plazme oviec po osetrenĭ chlormadinonacetátom (CAP) v dvoch synchronizovaných cykloch.