[The reactive behaviour of the cell nucleus after a pretreatment with a bromide solution and the application of the calcium-cobalt-method for the ATPase evidence (author's transl)].
A selective staining of the nucleus of both the cells in an islet (of Langerhans) and the acinar cells could be observed after treatment with bromide-water in an ATPase-medium (calcium-cobalt-method) on a cryostatsection of rat pancreas. Furthermore the behaviour of the other metal ion forming a complex has been tested in place of cobalt salt. As conditioned for the staining reaction the hydrolysis of nucleic acid with oxidation of its base by bromide-water and also the complex formation of Co++ with compounds similar to alloxan, resulted from oxidation were discussed.
D000251:Adenosine Triphosphatases / Q000032:analysis*; D000818:Animals; D001965:Bromides; D002118:Calcium; D002467:Cell Nucleus / Q000201:enzymology*; D003035:Cobalt; D006651:Histocytochemistry; D007515:Islets of Langerhans / Q000201:enzymology / Q000648:ultrastructure; D010179:Pancreas / Q000201:enzymology* / Q000648:ultrastructure; D051381:Rats; D013194:Staining and Labeling
D001965:Bromides; D003035:Cobalt; D000251:Adenosine Triphosphatases; D002118:Calcium
Das färberische Verhalten der Zellkerne nach Bromwasservorbehandlung und Anwendung der Calcium-Cobalt-Methode zum ATPaseNachweis.