[Fractionation of the Trichinella spiralis antigen and allergy diagnosis trails].
Experiments were carried out to fractionate an antigen of Trichinella spiralis with Sephadex G-50 and G-100. The specificty of the fractions has been followed up by means of the intradermal allergic reaction in six pigs, experimentally infected with Trichenalla spiralis, one with Trichocephalus suis and three, born by trichinellosis-affected sows. The second fraction has been tested also on four pigs, experimentally inoculated with Ascaris suum, nine--with Ascarsis suum and Oesophagostomum and 19 healthy pigs. The intradermal allergic tests were effected with thrichinellous fractions, diluted 1:1000. The results obtained from the investigations indicate that the best separation of the complete trichinellous antigen, obtained from lyophilized trichinellae, is achieved with Sephadex G-100. In the case of an intradermal allergic test, a well-expressed reaction is attained with the 2nd fraction and the 1st fraction, the reaction being maintained for a longer times with the 2nd fraction.
D000818:Animals; D000941:Antigens / Q000302:isolation & purification*; D001196:Ascariasis / Q000662:veterinary; D000939:Epitopes; D006967:Hypersensitivity / Q000662:veterinary*; D009814:Oesophagostomiasis / Q000662:veterinary; D012882:Skin Tests; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis*; D014234:Trichinella / Q000276:immunology*; D014235:Trichinellosis / Q000662:veterinary*
D000941:Antigens; D000939:Epitopes
Fraktsionirane na antigen ot Trichinella spiralis i opiti za alergichna diagnostika.