Purification of the pregnancy zone protein by affinity chromatography.
A method for purification of the pregnancy zone protein (PZP) by affinity chromatography was developed. A monospecific immunoglobulin fraction, covalently coupled to Sepharose 4B, was used as binding agent and the elution conditions for PZP are described. The purified protein was shown to have identical properties compared to native PZP with regard to molecular weight, immunodiffusion precipitation and immunosuppressive activity.
D000510:Alpha-Globulins / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007166:Immunosuppressive Agents / Q000302:isolation & purification; D011247:Pregnancy; D011485:Protein Binding
D000510:Alpha-Globulins; D007166:Immunosuppressive Agents