Uridine kinase activities in normal and neoplastic lymphoid cells.
Both adult (I) and embryonic (II) forms of uridine kinase have been identified in the transplantable EL-4 leukemia of C57BL/6 mice and in the P815Y mastocytoma of DBA/2 mice. Only Species I is found in primary tumor cells of lymphoid orgin (virus-induced feline lymphosarcoma, human acute and chronic lymphocytic leukemia) and in normal calf thymocytes and porcine peripheral blood lymphocytes; Species I was induced 4-fold upon stimulation of the normal blood lymphocytes with phytohemagglutinin. The level of uridine kinase activity in the feline lymphosarcoma of thymus-dependent lymphocyte orgin and childhood lymphocytic leukemia of possible thymus-dependent lymphocyte or null-cell origin was similar to the induced level in phytohemagglutinin-stimulated normal lymphocytes, i.e., thymus-dependent lymphocytes. In contrast lymphocytes of a patient with chronic lymphocytic leukemia of thymus-independent lymphocyte origin had a level of uridine kinase activity comparable to that of the unstimulated normal lymphocytes or thymocytes. The uridine kinase activity in the EL-4 tumor cells was repressed by acute treatment of the mice with 5-azacytidine.
D000818:Animals; D001374:Azacitidine / Q000494:pharmacology; D001402:B-Lymphocytes / Q000201:enzymology; D006528:Carcinoma, Hepatocellular / Q000201:enzymology; D002415:Cats; D004790:Enzyme Induction / Q000187:drug effects; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism*; D007942:Leukemia, Experimental / Q000201:enzymology; D007945:Leukemia, Lymphoid / Q000201:enzymology; D008113:Liver Neoplasms / Q000201:enzymology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000201:enzymology; D008228:Lymphoma, Non-Hodgkin / Q000201:enzymology; D008297:Male; D012515:Mast-Cell Sarcoma / Q000201:enzymology; D051379:Mice; D008875:Middle Aged; D009369:Neoplasms / Q000201:enzymology*; D009374:Neoplasms, Experimental / Q000201:enzymology; D010770:Phosphotransferases / Q000378:metabolism*; D051381:Rats; D013601:T-Lymphocytes / Q000201:enzymology; D014541:Uridine Kinase / Q000096:biosynthesis / Q000378:metabolism*
D007527:Isoenzymes; D010770:Phosphotransferases; D014541:Uridine Kinase; D001374:Azacitidine