[Heterogeneity of the cellular distribution of erythrocytic A antigens. Ultramicroscopic study].
A and A1 antigens have been detected on cells of the human erythrocyte series by immunoelectron microscopy. These antigens have been revealed by an indirect method involving various anti-A and anti-A1 antibodies (allo, auto, hetero-antibodies) and peroxidase-conjugated anti-immunoglobulin antibodies. Immunologic labelling has been carried out with erythrocyte or bone marrow cell suspensions which were fixed prior to incubation with reagents. Cells from various A phenotypes were examined. A and A1 antigens were visualized on maturing normoblasts, at every developmental stage. In addition cell to cell variations of the surface labelling of erythrocytes was found in normal phenotypes, suggesting the existence of several populations of cells according to antigenic load.
D000017:ABO Blood-Group System; D002462:Cell Membrane / Q000276:immunology / Q000648:ultrastructure; D004900:Erythroblasts / Q000276:immunology / Q000648:ultrastructure; D014644:Genetic Variation; D006801:Humans; D007519:Isoantigens / Q000032:analysis*; D008854:Microscopy, Electron; D010641:Phenotype; D013194:Staining and Labeling
D000017:ABO Blood-Group System; D007519:Isoantigens
L'hétérogénéité de la distribution cellulaire des antigènes A érythrocytaires. Etude au microscope électronique