The determination of serum immunoglobulin concentrations on the basis of their light-chain antigenic properties.
A method for the measurement of light-chain determined immunoglobulin concentrations (Ig-type  kappa and Ig-type lambda) in serum is described. The method is based on the principle of radial immunodiffusion. The results obtained after calibration with standarized human serum and with isolated Bence-Jones protein are closely correlated. The values for Ig-type kappa, Ig-type lambda and the kappa/lambda ratio from sera with normal concentrations of IgG, IgA and IgM are presented and some problems of calibration discussed.
D000293:Adolescent; D000328:Adult; D001536:Bence Jones Protein / Q000032:analysis; D000939:Epitopes; D006801:Humans; D005779:Immunodiffusion; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007147:Immunoglobulin Light Chains / Q000032:analysis*; D007075:Immunoglobulin M / Q000032:analysis; D007145:Immunoglobulin kappa-Chains / Q000032:analysis*; D007146:Immunoglobulin lambda-Chains / Q000032:analysis*; D007136:Immunoglobulins / Q000032:analysis*; D008875:Middle Aged
D000939:Epitopes; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007147:Immunoglobulin Light Chains; D007075:Immunoglobulin M; D007145:Immunoglobulin kappa-Chains; D007146:Immunoglobulin lambda-Chains; D007136:Immunoglobulins; D001536:Bence Jones Protein