Concanavalin A-peroxidase-diaminobenzidine-periodic acid-m-aminophenol-fast black salt K: a method for the dual staining of neutral complex carbohydrates.
A method has been developed for the dual staining of neutral complex carbohydrates in light microscopy. It combines a concanavalin A-peroxidase-diaminobenzidine (Con A-PO-DAB) method with a period acid-m-aminophenol-Fast Black salt K (PA-AP-FBK) sequence. With the combined method it is possible to stain alpha-D-glycosyl and alpha-D-mannosyl residues brown and 1,2-glycol groups of neutral complex carbohydrates blackish purple. The validity of the method has been confirmed with appropriate histochemical controls and enzyme digestions on test tissues.
D015100:3,3'-Diaminobenzidine; D000627:Aminophenols; D000818:Animals; D002241:Carbohydrates / Q000032:analysis*; D002417:Cattle; D004396:Coloring Agents; D003208:Concanavalin A; D004285:Dogs; D006651:Histocytochemistry; D006735:Horseradish Peroxidase; D006801:Humans; D051379:Mice; D009928:Organ Specificity; D010504:Periodic Acid; D011817:Rabbits; D051381:Rats; D013194:Staining and Labeling / Q000379:methods*
D000627:Aminophenols; D002241:Carbohydrates; D004396:Coloring Agents; D010504:Periodic Acid; D003208:Concanavalin A; D015100:3,3'-Diaminobenzidine; D006735:Horseradish Peroxidase