Differential fluorescence of sister chromatids with 4'-6-diamidino-2-phenylindole.
Differential fluorescence of sister chromatids and sister chromatid exchanges (SCE) in chromosomes from human lymphocytes grown two replication cycles in medium containing 5-bromodeoxyuridine can be detected by fluorescence microscopy after staining with 4'-6-diamidino-2-phenylindole (DAPI). The DAPI fluorescence appears to be more stable than that of the dye 33258 Hoechst and may provide a more sensitive method for the detection of SCE.
D002842:Chromatids / Q000502:physiology; D003434:Crossing Over, Genetic; D005453:Fluorescence; D005456:Fluorescent Dyes; D006801:Humans; D007962:Leukocytes / Q000648:ultrastructure*; D013194:Staining and Labeling
D005456:Fluorescent Dyes