Effects of DAPI on human leukocytes in vitro.
DAPI (4'-6-diamidino-2-phenylindole), a fluorochrome specific for AT-rich DNA, was supplied for 24 h at various concentrations to human leukocytes in culture. This treatment caused the appearance on the chromosomes of specific areas lacking spiralization. In particular, the centromeric regions of chromosomes 1,9, and 16, a short region on the long arm of chromosomes 1 and 2, and the distal heterochromatic part of the long arm of the Y chromosome were despiralized. The despiralization pattern of DAPI is compared with those previously obtained with Hoechst 33258 and Distamycin A.
D006690:Bisbenzimidazole / Q000494:pharmacology; D002478:Cells, Cultured; D002877:Chromosomes, Human / Q000187:drug effects*; D003470:Culture Media; D004247:DNA / Q000378:metabolism; D004214:Distamycins / Q000494:pharmacology; D006801:Humans; D007211:Indoles / Q000008:administration & dosage / Q000494:pharmacology*; D007962:Leukocytes / Q000187:drug effects / Q000648:ultrastructure*; D008297:Male; D008677:Metaphase / Q000187:drug effects
D003470:Culture Media; D004214:Distamycins; D007211:Indoles; D004247:DNA; D006690:Bisbenzimidazole