Antigen-specific T cell factors: a fine analysis of specificity.
The specificity of T cell factors produced in presence of synthetic polypeptide antigens was studied. Factors prepared with either one of the three antigens: poly(Tyr,Glu)-poly(DLALa)--poly(Lys), (T,G)-A--L, poly(Phe,Glu)-poly(DLALa)--poly(Lys), (Phe,G)-A--L, and poly(His,Glu)-poly(DLALa)--poly(Lys), (H,G)-A--L, successfully cooperated with B cells for antibody production to the homologous as well as to the other two immunogens. Furthermore, the activity of a (T,G)-A--L-specific factor was removed after passage through immunoadsorbents built of Sepharose coupled to: (T,G)A--L, (Phe-G)-A--L and poly(Glu)-poly(DLAa)--poly(Lys), (G)-A--L, but not to poly (DLALa)--poly(LLys),A--L. No cross-reactivity was observed between (T,G)-A--L and poly(Tyr,Glu)-poly(Pro)--poly(Lys), (T,G)-Pro--L, at the level of T cell factors, as shown using the above approaches. These results lead to the conclusion that specificity of T cell factors, although not identical, is similar to that of antibodies.
D000818:Animals; D000917:Antibody Formation; D000937:Antigen-Antibody Reactions; D001402:B-Lymphocytes / Q000276:immunology; D003429:Cross Reactions; D000939:Epitopes; D007163:Immunosorbent Techniques; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D010455:Peptides / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000939:Epitopes; D010455:Peptides