Cytotoxic effect of a reverse transcriptase inhibitor, AF/ABDP cis, on ovaries of young Xenopus laevis--ultrastructural and autoradiographic study.
Observations with the light and electron microscopes showed that a reverse transcriptase inhibitor, AF/ABDP cis which is a rifampicin derivative, had a toxic effect on ovaries of young Xenopus laevis. It induced mitochondrial damage in all observed cells and marked alteration of the ultrastructure of nucleus of the pachytene oocyte. Light autoradiography showed that AF/ABDP cis caused wide variation in nuclear labelling with [3H] thymidine from one pachytene oocyte to another. Somatic cells were more uniformly labelled. These results are discussed in relation to the interpretation experiments upon eucaryote cells in which this drug is used as a reverse transcriptase inhibitor.
D000818:Animals; D001345:Autoradiography; D005260:Female; D066298:In Vitro Techniques; D008854:Microscopy, Electron; D009865:Oocytes / Q000187:drug effects / Q000648:ultrastructure; D010053:Ovary / Q000187:drug effects* / Q000648:ultrastructure; D018894:Reverse Transcriptase Inhibitors; D012293:Rifampin / Q000031:analogs & derivatives* / Q000494:pharmacology; D014981:Xenopus
D018894:Reverse Transcriptase Inhibitors; D012293:Rifampin