ATPase activity in the breast: a comparison between three methods.
Adenosine triphosphatase enzymatic activity was investigated in human approximatively normal, dysplastic and neoplastic mammary tissue, by three different methods. Staining intensity varied within wide limits; myoepithelial cells and blood vessels showed similar enzymatic activity. Epithelial cells reacted only faintly, or not at all; carcinoma cells were never labelled. Stromal response was highly variable. The calcium-cobalt method of Padykula and Herman gave more intense reactions than the lead-nitrate procedure of Wachstein and Meisel, either in the original form or according to the modifications recommended by Russo and Wells. With the latter method the sharpness of stain deposits on the different structures was markedly enhanced. The functional significance of ATPase activity is discussed.
D000251:Adenosine Triphosphatases / Q000032:analysis*; D001940:Breast / Q000201:enzymology* / Q000648:ultrastructure; D000252:Calcium-Transporting ATPases / Q000032:analysis; D005260:Female; D006651:Histocytochemistry; D006801:Humans; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis; D013194:Staining and Labeling
D000251:Adenosine Triphosphatases; D000252:Calcium-Transporting ATPases; D000254:Sodium-Potassium-Exchanging ATPase