Rabbit antiserum against a non-T, non-B leukemia cell line that carries the Ph1 chromosome (NALM-1): antibody specific to a non-T, non-B acute lymphoblastic leukemia antigen.
Rabbit immune sera against human cells of a non-T, non-B leukemia cell line bearing the Ph1 chromosome (NALM-1) were characterized. After proper and exhaustive absorption, the sera were tested by indirect membrane immunofluorescence on a panel of 19 human hematopoietic cell lines with T-, B-, or non-T, non-B cell surface characteristics. The absorbed sera reacted specifically with NALM-1 cells but not with cells of another Ph1-positive cell line, K-562. Four of the 6 leukemia T-cell lines, 2 of the 4 leukemia-lymphoma B-cell lines, all of 4 acute lymphoblastic leukemia (ALL) lines with non-T, non-B cell characteristics, and uncultured cells of all patients with non-T, non-B cell ALL were reactive with the antisera. A cross-absorption study of the antisera suggested that a single antigenic complex is involved in this antigen specificity. The antigen involved appears to be a common non-T, non-B ALL one that has been described previously.
D000818:Animals; D000912:Antibodies, Neoplasm; D000918:Antibody Specificity; D000951:Antigens, Neoplasm; D001402:B-Lymphocytes / Q000276:immunology; D002460:Cell Line; D002869:Chromosome Aberrations; D002904:Chromosomes, Human, 21-22 and Y; D000939:Epitopes; D005260:Female; D006801:Humans; D007942:Leukemia, Experimental / Q000235:genetics / Q000276:immunology*; D007945:Leukemia, Lymphoid / Q000235:genetics / Q000276:immunology*; D008297:Male; D011817:Rabbits; D013601:T-Lymphocytes / Q000276:immunology
D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D000939:Epitopes