Reduction of the non-specific binding of DNA to gamma-globulin in Farr radioimmunoassay by addition of dextran sulfate and calcium chloride.
The effect of non-specific binding caused by the interaction between gamma-globulin and denatured DNA was markedly reduced by addition of dextran sulfate or CaCl2 at alkaline pH. This method was shown to be applicable in the detection of anti-DNA antibodies in sera from cases of human systemic lupus erythematosus.
D002122:Calcium Chloride; D004247:DNA / Q000378:metabolism*; D003911:Dextrans; D008722:Methods; D011485:Protein Binding; D011863:Radioimmunoassay; D005719:gamma-Globulins / Q000378:metabolism*
D003911:Dextrans; D005719:gamma-Globulins; D004247:DNA; D002122:Calcium Chloride