Surface immunoglobulin on rabbit lymphoid cells. V. Ultrastructural distribution of b4 allotypic determinants of thymus, lymph node and bone marrow lymphocytes.
An indirect immunoferritin-labeling technique is used to localize cell surface immunoglobulin (Ig) allotypic determinants on rabbit lymph node, bone marrow and thymus lymphocytes. 47% of the lymph node cells, 62% of bone marrow small lymphocytes and less than 1% of thymic lymphocytes, are positive for surface Ig. Two Ig-positive lymph node lymphocyte populations which differ in fine structure are identified but both cell types express similar amounts of surface Ig (7,000-28,000 Ig molecules per cell). Bone marrow small lymphocytes make up only 9% of the total cell population and express less surface Ig (ca. 3,000-12,000 molecules Ig per cell) than most Ig-bearre described and both are essentially negative for surface Ig using this labeling technique.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D001853:Bone Marrow / Q000276:immunology / Q000648:ultrastructure; D001854:Bone Marrow Cells; D002462:Cell Membrane / Q000276:immunology / Q000648:ultrastructure; D002467:Cell Nucleus / Q000648:ultrastructure; D000939:Epitopes; D005293:Ferritins / Q000276:immunology; D007126:Immunoglobulin Allotypes; D007141:Immunoglobulin Fc Fragments; D007145:Immunoglobulin kappa-Chains; D008198:Lymph Nodes / Q000276:immunology / Q000648:ultrastructure; D008214:Lymphocytes / Q000276:immunology* / Q000648:ultrastructure; D008928:Mitochondria / Q000648:ultrastructure; D011817:Rabbits; D011947:Receptors, Antigen, B-Cell / Q000032:analysis*; D012270:Ribosomes / Q000648:ultrastructure; D013950:Thymus Gland / Q000276:immunology / Q000648:ultrastructure
D000888:Antibodies, Anti-Idiotypic; D000939:Epitopes; D007126:Immunoglobulin Allotypes; D007141:Immunoglobulin Fc Fragments; D007145:Immunoglobulin kappa-Chains; D011947:Receptors, Antigen, B-Cell; C496591:kappa allotype b4, rabbits; D005293:Ferritins