Immunologic tolerance to heterologous immunoglobulin: its relation to in vitro filtration by macrophages.
The cellular and molecular basis for the difference in ability of BCG to induce tolerance in BALB/c and DBA/2 mice has been examined by in vitro biofiltration. It was found that incubation with the adherent cells from BALB/c but not DBA/2 spleens could remove the material from BGG which inhibited tolerance induction in BALB/c mice. This material was shown to represent only a trace component in BGG, was present in only certain commercial batches of BGG, and was apparently unrelated to the presence of aggregates or endotoxin.
D000818:Animals; D002417:Cattle; D002448:Cell Adhesion; D002469:Cell Separation; D007108:Immune Tolerance; D007122:Immunoelectrophoresis; D008070:Lipopolysaccharides / Q000032:analysis; D008264:Macrophages / Q000502:physiology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D013154:Spleen / Q000276:immunology; D005719:gamma-Globulins / Q000032:analysis / Q000494:pharmacology*
D008070:Lipopolysaccharides; D005719:gamma-Globulins