Thy-L antigen expression on rat brain cell lines.
Cell lines derived from the central nervous system of rats were screened serologically for the presence of Thy-1 (theta), a cell surface differentiation antigen shared by brain and thymus of rats and mice. Both cytotoxicity absorption and indirect immunofluorescence assays were performed using a rabbit anti-rat thymocyte serum (ATS) with Thy-1 specificity. The complement-dependent cytotoxicity of ATS detected a mouse-rat cross-reacting determinant of the molecule bearing the Thy-1 antigen. Of 20 lines tested, 1 of 6 neuronal and 7 of 14 non-neuronal lines expressed Thy-1, as judged by their capacity to absorb ATS cytotoxicity for a Thy-1 positive thymoma line. Similar results were obtained in quantitative absorption assays of these lines employing a mouse anti-Thy 1.1 alloantiserum, but the xenoantiserum (ATS) was more sensitive for detecting the rat molecule bearing Thy-1. Indirect immunofluorescence, which was performed on several of the lines, yielded results in complete agreement with the cytotoxicity absorption assays, and revealed a generalized distribution of antigen in a speckled or patchy pattern over the membrane of cell bodies and processes.
D000818:Animals; D001921:Brain / Q000276:immunology*; D002460:Cell Line; D003429:Cross Reactions; D003602:Cytotoxicity, Immunologic; D000939:Epitopes; D005455:Fluorescent Antibody Technique; D007519:Isoantigens / Q000032:analysis*; D051379:Mice; D008806:Mice, Inbred AKR; D009457:Neuroglia / Q000276:immunology; D009474:Neurons / Q000276:immunology; D011817:Rabbits; D051381:Rats; D011917:Rats, Inbred Lew; D013950:Thymus Gland / Q000276:immunology
D000939:Epitopes; D007519:Isoantigens