title: Human B cell line deficient in the expression of B cell-specific glycoproteins (GP 27,35).
abstract: A human B lymphoid cell line, P3HR-1, expresses only low levels of the 27 000 and 35 000 mol.wt. B cell-specific glycoproteins (GP 27,35). Indirect antibody-binding and quantitative absorption tests with a xenoantiserum against the antigens showed that P3HR-1 cells have on their surface about 1% of the amount found on other human B lymphoblastoid cell lines. The deficit of the glycoproteins on the surface of P3HR-1 cells could be accounted for by a reduced rate of synthesis in these cells. A simple relationship between the reduced expression of GP 27,35 on P3HR-1 cells and their inability to bind Epstein-Barr virus (EBV) or express complement receptors was excluded because other B lymphoid cells which expressed neither virus-binding sites nor complement receptor had normal amounts of GP 27,35 on their surface. However, antibodies against GP 27,35 could block the absorption of EBV by EBV receptor-positive B cells.
mesh_terms: D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D001665:Binding Sites; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D002471:Cell Transformation, Neoplastic; D003165:Complement System Proteins / Q000378:metabolism; D000939:Epitopes; D006023:Glycoproteins / Q000276:immunology* / Q000378:metabolism; D004854:Herpesvirus 4, Human / Q000302:isolation & purification; D006801:Humans; D008565:Membrane Proteins / Q000276:immunology / Q000378:metabolism; D008970:Molecular Weight
chemical_list: D000939:Epitopes; D006023:Glycoproteins; D008565:Membrane Proteins; D003165:Complement System Proteins