title: Determination of the number of active saprophytic aquatic bacteria by semi-continuous culture on membrane filters.
abstract: The number of bacteria in the hypolimnion waters of Lake Mikpłajski was examined by direct count on membrane filters, by the agar plate method and by semi-continuous culture on membrane filters, as previously proposed by the author. The agar plate method recovered only 20--25% of the bacteria found by direct method. On the other hand, semi-continuous culture on membrane filters allowed the recovery of about 90% of the total count. At the same time it differentiated between active, microcolony forming cells (about 70%) and inactive single cells (about 20% of the total count).
mesh_terms: D001419:Bacteria / Q000254:growth & development*; D001431:Bacteriological Techniques; D003470:Culture Media; D008851:Micropore Filters; D014871:Water Microbiology
chemical_list: D003470:Culture Media