Immunological studies on CS-1170, a new semisynthetic cephamycin antibiotic.
The antigencity of CS-1170, a newly developed semi-synthetic cephamycin, and it's cross-reactivity with beta-lactam antibiotics were studied. The antigencity was confirmed by the following tests: 1)the passive hemagglutination test with anti-CS-1170 antisera of guinea pigs immunized with CS-1170 plus Freund complete adjuvant, 2)lymphocyte proliferation response in vitro stimulated with this drug in the guinea pigs, 3) the PCA test using mice immunized with CS-1170-protein conjugates. The antibody to CS-1170 appears to be directed to the acyl side chain because cyanomethylthioacetylglycine, an univalent acyl side chain of the CS-1170, can significantly cross-react with the antibody. A methoxy group on the C-7 alpha-position of the antibiotic plays no important part for the antigenic specificity of the molecule. The cross-reactivity of CS-1170 with cefazolin, cephalothin, benzylpenicillin and ampicillin was only to a minimal extent in the passive hemagglutination test. The cross-reactivity of CS-1170 and the related antibiotics was not observed among them in the PCA system with IgE anti-CS-1170. These findings support a conclusion that CS-1170 is one of beta-lactam antibiotic with an immunologically minimal cross-reactivity to the related antibiotics.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D002511:Cephalosporins / Q000276:immunology*; D002513:Cephamycins / Q000276:immunology*; D003429:Cross Reactions; D000939:Epitopes; D006168:Guinea Pigs; D006241:Haptens / Q000276:immunology; D006384:Hemagglutination; D007073:Immunoglobulin E / Q000032:analysis; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D010406:Penicillins / Q000276:immunology
D000906:Antibodies; D002511:Cephalosporins; D002513:Cephamycins; D000939:Epitopes; D006241:Haptens; D010406:Penicillins; D007073:Immunoglobulin E