[Separation of the biosynthesis products of a mutant strain of Actinomyces chrysomallus var. carotenoides and the identification of actinomycin antibiotics].
An orange antibiotically active substance isolated from the mycelium of a mutant strain of Actinomyces chrysomallus var. carotenoides was identified as a mixture of actinomycins according to its light absorption spectra, circular dichroism spectra, IR spectra and chromatographic comparison with the standard samples. A scheme for successive extraction of the biologically active substances from the mycelium resulting in isolation of a fraction enriched with antibiotic substances and a fraction enriched with pigments is presented. A method for separation and purification of 3 groups of biologically active substances from the mycelium enriched extract was developed.
D000903:Antibiotics, Antineoplastic / Q000302:isolation & purification*; D002942:Circular Dichroism; D003470:Culture Media; D003609:Dactinomycin / Q000032:analysis* / Q000096:biosynthesis; D005419:Flavonoids / Q000096:biosynthesis; D009154:Mutation; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet; D013302:Streptomyces / Q000378:metabolism*
D000903:Antibiotics, Antineoplastic; D003470:Culture Media; D005419:Flavonoids; D003609:Dactinomycin
Razdelenie produktov biosinteza mutantnogo shtamma Actinomyces chrysomallus var. carotenoides i identifikatsiia antibiotikov aktinomitsinov.