Association of melanoma tumor antigen activity with beta2-microglobulin.
The majority of melanoma tumor antigen activity present in melanoma extracts derived from fresh tumor tissue binds to a Sepharose-anti-beta2-microglobulin adsorbent. Removal of HLA antigens from the extracts of melanoma tissue by using a KBr flotation technique did not reduce either the tumor antigen activity of the extracts or the binding of melanoma tumor antigen (MTA) activity to the Sepharose-anti-beta2-microglobulin adsorbent. The complete blocking of MTA activity by pretreating the anti-beta2-microglobulin adsorbent with beta2-microglobulin and the lack of detectable MTA binding to a Sepharose anti-normal human serum adsorbent demonstrated the specificity of the binding of MTA to the anti-beta2-microglobulin adsorbent.
D000951:Antigens, Neoplasm / Q000008:administration & dosage / Q000302:isolation & purification*; D001609:Beta-Globulins / Q000276:immunology*; D006680:HLA Antigens; D006801:Humans; D007163:Immunosorbent Techniques; D007428:Intradermal Tests; D007957:Leukocyte Adherence Inhibition Test; D008545:Melanoma / Q000276:immunology*; D001613:beta 2-Microglobulin / Q000276:immunology*
D000951:Antigens, Neoplasm; D001609:Beta-Globulins; D006680:HLA Antigens; D001613:beta 2-Microglobulin