Structural studies on induced antibodies with defined idiotypic specificities. V. The complete amino acid sequence of the light chain variable regions of anti-p-azophenylarsonate antibodies from A/J mice bearing a cross-reactive idiotype.
The complete amino acid sequence of the variable regions of light chains derived from anti-p-azophenylarsonate antibodies from A/J mice bearing a cross-reactive idiotype is reported. At least two and probably more than three distinct light chains are associated with this idiotypically characterized antibody. The antibodies have several differences in their "framework" structures but evidence is presented indicating that all three light chain hypervariable regions have a homogeneous sequence. The data are discussed in relation to the various theories of antibody diversity. In addition, the findings support the view that hypervariable regions, idiotypic determinants, and the antibody-combining site involve, to a large extent, the same molecular structures.
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000032:analysis*; D001391:Azo Compounds / Q000276:immunology*; D001666:Binding Sites, Antibody; D002850:Chromatography, Gel; D002949:Citraconic Anhydrides / Q000494:pharmacology; D003429:Cross Reactions; D000939:Epitopes; D005838:Genotype; D007147:Immunoglobulin Light Chains / Q000032:analysis*; D007135:Immunoglobulin Variable Region; D051379:Mice; D008805:Mice, Inbred A; D010455:Peptides / Q000032:analysis; D014357:Trypsin / Q000494:pharmacology; D010132:p-Azobenzenearsonate / Q000276:immunology*
D000906:Antibodies; D001391:Azo Compounds; D002949:Citraconic Anhydrides; D000939:Epitopes; D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region; D010455:Peptides; D010132:p-Azobenzenearsonate; D014357:Trypsin