Genetics of cell-mediated lympholysis in man.
Cell-mediated lympholysis (CML) was studied in a family containing two siblings in who genetic recombinaiton had occurred in the HLA comples. In one sibling, recombination occurred between the HLA-A locus and the HLA-B locus. In the second sibling recombination occurred between the HLA-B locus and the HLA-D locus. Strong CML activity was generated in mixed lymphocyte cultures (MLC) when stimulator and responder cells differed in HLA-A, B, and D antigens. MLC involving HLA-D differences alone did not generate CML. Weak, but definite CML activity was generated during MLC with cells differing at HLA-A and HLA-B but sharing HLA-D. HLA-B antigens were good targets for lysis in all combinations studied. HLA-A antigens were poor targets in some but not in all combinations. However, combinations where HLA-A antigens seemed to be good targets could have involved HLA-B differences due to polymorphism of HLA-B7 antigens each inherited from a different parent. HLA-D antigens did not serve as targets for lysis. In three cell experiments, excellent CML activity was generated when responder cells were stimulated by HLA-D antigens and by HLA-A and B antigens present on separate stimulator cells.
D000328:Adult; D002648:Child; D003601:Cytotoxicity Tests, Immunologic; D000939:Epitopes; D005260:Female; D005796:Genes; D005838:Genotype; D006680:HLA Antigens; D006649:Histocompatibility Antigens; D006801:Humans; D007111:Immunity, Cellular; D007519:Isoantigens; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D011995:Recombination, Genetic
D000939:Epitopes; D006680:HLA Antigens; D006649:Histocompatibility Antigens; D007519:Isoantigens