Separation of fucosyltransferase and endogenous acceptors of fucose from human parotid saliva.
Using gel filtration on Sephadex G-100 and column chromatography on CM-cellulose, fractions containing either endogenous acceptor of L-fucose or fucosyltransferase were obtained from human parotid saliva. Hydrolysis with mild acid followed by Smith degradation increased the fucosyltransferase activity of the fractions that contained endogeneous acceptor.
D000328:Adult; D005643:Fucose / Q000378:metabolism*; D006023:Glycoproteins; D006154:Guanosine Diphosphate Fucose; D006602:Hexosyltransferases / Q000302:isolation & purification*; D006801:Humans; D010306:Parotid Gland; D012463:Saliva / Q000032:analysis* / Q000201:enzymology; D012471:Salivary Proteins and Peptides / Q000378:metabolism; D000509:alpha-Fetoproteins / Q000378:metabolism
D006023:Glycoproteins; D012471:Salivary Proteins and Peptides; D000509:alpha-Fetoproteins; D006154:Guanosine Diphosphate Fucose; D005643:Fucose; D006602:Hexosyltransferases