Growth of embryonal carcinoma cells in serum-free medium.
Two mouse embryonal carcinoma cell lines, PCC.4 aza-1 and F9, have been grown in serum-free F-12 medium supplemented with Pedersen fetuin, insulin, transferrin, and 2-mercaptoethanol. This medium supports long-term growth of both cell lines. When these cells are transferred from medium containing serum to this serum-free medium, growth continues without any detectable lag. PCC.4 aza-1 grown in this medium for over 20 generations retains the capacity to differentiate in vivo. This medium appears to be a general serum-free medium for the growth of embryonal carcinoma cells.
D001769:Blood; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003470:Culture Media; D007328:Insulin / Q000494:pharmacology; D008623:Mercaptoethanol / Q000494:pharmacology; D013724:Teratoma / Q000473:pathology / Q000503:physiopathology*; D014168:Transferrin / Q000494:pharmacology; D000509:alpha-Fetoproteins / Q000494:pharmacology*
D003470:Culture Media; D007328:Insulin; D014168:Transferrin; D000509:alpha-Fetoproteins; D008623:Mercaptoethanol