Effect of growth-promoting alpha-globulin on the incorporation of exogenous DNA into L cells. II. Fate of exogenous 3H-DNA after incorporation into recipient cells.
GPAG stimulates the uptake of exogenous 3H-DNA by L cells and facilitates its retention in host cell nuclei during 48-h postcultivation; the nuclei contain 1.5 times more radioactivity than GPAG-free controls. Owing to the well-known properties of GPAG, the formation of the 3H-DNA-GPAG complex may affect the incorporation of exogenous 3-H-DNA and its intracellular transport and retention in the host cell nuclei.
D000510:Alpha-Globulins / Q000494:pharmacology*; D000818:Animals; D004247:DNA / Q000378:metabolism*; D003851:Deoxyribonucleases / Q000494:pharmacology; D006133:Growth Substances / Q000494:pharmacology*; D007739:L Cells / Q000378:metabolism*; D051379:Mice
D000510:Alpha-Globulins; D006133:Growth Substances; D004247:DNA; D003851:Deoxyribonucleases