title: Immunochemical study of beta-glucuronidase inhibitor from porcine sublingual gland. Relationship between the antigenic determinant and the active site of the inhibitor.
abstract: Following removal of sialic acid by neuraminidase treatment the activity of the beta-glucuronidase inhibitor was remarkably decreased, but the antigenic determinant was not affected. A partial common antigen to the inhibitor was isolated from porcine small intestine, by successive fractionation of trypsin extraction of the latter on Sephadex G-150, DEAE-cellulose and Sepharose 4B column chromatography. The immunologic and characteristic properties of the common antigen were compared with those of the beta-glucuronidase inhibitor is not identical with its antigenic determinant.
mesh_terms: D000818:Animals; D000941:Antigens / Q000302:isolation & purification; D001665:Binding Sites; D000939:Epitopes; D005966:Glucuronidase / Q000037:antagonists & inhibitors*; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007421:Intestine, Small / Q000276:immunology; D009439:Neuraminidase; D011506:Proteins / Q000276:immunology / Q000502:physiology; D012794:Sialic Acids / Q000032:analysis; D013361:Sublingual Gland / Q000032:analysis*; D013552:Swine
chemical_list: D000941:Antigens; D000939:Epitopes; D011506:Proteins; D012794:Sialic Acids; D009439:Neuraminidase; D005966:Glucuronidase