title: Surface coats on human lymphocytes: freeze-drying and staining with cations.
abstract: Surface coats can be demonstrated on human peripheral blood lymphocytes by staining with ruthenium red, alcian blue, Thorotrast, and cationized ferritin, which are similar in distribution to a 40- to 65-nm layer of amorphous extracellular material recently reported on fixed, freeze-dried lymphocytes. Several additional lines of evidence, including X-ray microanalysis, suggest that the latter is not a contaminant added by freeze-drying. Freeze-drying may provide the means for a morphological assessment of the lymphocyte surface, including the extracellular coat, which may give additional insight into the immune response.
mesh_terms: D000328:Adult; D002462:Cell Membrane / Q000648:ultrastructure*; D005612:Freeze Drying; D006801:Humans; D008214:Lymphocytes / Q000648:ultrastructure*; D013194:Staining and Labeling