The temperature dependence of the surface tension of aqueous solutions of plasma proteins.
The surface behavior of aqueous solutions of fibrinogen, transferrin, gamma-globulin and albumin at the liquid-gas interface has been investigated by a modified Wilhelmy technique. The temperature dependence of the surface tension was studied over a temperature range of 20--80 degrees C and a pH range of 2--12. Most pronounced conformational changes of fibrinogen with this technique were found in physiological conditions: 35--45 degrees C and pH 7--8. A conformational change was found for gamma-globulin and transferrin solutions, but at a higher temperature and less pronounced than fibrinogen. Albumin did not undergo conformational transitions to a significant extent.
D005340:Fibrinogen; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D012710:Serum Albumin, Bovine; D013500:Surface Tension; D013696:Temperature; D014168:Transferrin; D005719:gamma-Globulins
D014168:Transferrin; D005719:gamma-Globulins; D012710:Serum Albumin, Bovine; D005340:Fibrinogen