Nonspecific inhibition of tumor growth in vivo by admixed allogeneic tumor-sensitized lymphoid cells and identical inactivated allogeneic tumor cells.
With the in vivo tumor neutralization test (Winn test), growth of a transplanted (KMT-17) from Wistar-King-Aptekman rats was inhibited by allogeneic tumor (AH-66 from Donryu rats)-sensitized syngeneic lymphoid cells admixed with mitomycin C (MMC)-treated AH-66 cells. The observed tumor inhibition may be immunologically nonspecific, since no cross-antigens were detected by membrane immunofluorescence on the surfaces of KMT-17 and AH-66 cells. Close contact among KMT-17, AH-66-sensitized lymphoid cells and MMC-treated AH-66 cells was required for the inhibition of KMT-17 growth. AH-66 cells pretreated with formalin or ultrasonication lost tumor inhibitory activity when they were admixed with AH-66-sensitized lymphoid cells, and only MMC-treatment effectively preserved the tumor inhibitory activity of AH-66 cells. The sensitized spleen cells, draining lymph node, or peripheral blood cells inhibited tumor growth when they were admixed with MMC-treated AH-66 cells, whereas nucleated cells from bone marrow, thymus, or distal lymph node did not. Growths of KMT-17 were inhibited by admixed sensitized spleen cells and MMC-treated AH-66 even when pre-irradiated rats were used as recipients.
D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis; D002448:Cell Adhesion; D002471:Cell Transformation, Neoplastic; D003429:Cross Reactions; D004306:Dose-Response Relationship, Immunologic; D000939:Epitopes; D005455:Fluorescent Antibody Technique; D007942:Leukemia, Experimental / Q000276:immunology*; D008113:Liver Neoplasms / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology*; D008937:Mitomycins / Q000494:pharmacology; D009368:Neoplasm Transplantation; D009500:Neutralization Tests; D009928:Organ Specificity; D011828:Radiation Chimera; D051381:Rats; D012513:Sarcoma, Experimental / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D013997:Time Factors
D000951:Antigens, Neoplasm; D000939:Epitopes; D008937:Mitomycins