[Method of intravital injection of the internal blood vessels of organs for experimental-morphological research].
Ten minutes before the injection the rat is given heparin (500 units per 1 kg weight) intradermally. To the narcotized animal, the needle with the 1 ml syringe is inserted and 3--5 ml of blood are aspirated (about 2% of the body mass). Then with the same needle another syringe of 10 ml volume containing filtrated and heated up to 40 degrees C undiluted Indian ink is injected. The amount of the Indian ink injected is 8--10 ml per 100 g of the body mass. We can consider the injection oa success if the mucose of the tongue, the skin of the concha auriculae, the sclera of the eye are promptly stained during the injection and the tail vessels are well filled.
D000818:Animals; D001808:Blood Vessels / Q000033:anatomy & histology*; D004396:Coloring Agents / Q000008:administration & dosage; D006321:Heart; D007267:Injections; D051381:Rats; D013194:Staining and Labeling / Q000379:methods
D004396:Coloring Agents
Metodika prizhiznenno ĭ in'ektsii vnutriorgannykh krovenosnykh sosudov pri éksperimental'no-morfologicheskikh issledovaniiakh.