Specificity and structural analysis of a guinea pig transfer factor-like activity.
A transfer factor-like activity was prepared by Sephadex G-25 chromatography of immune guinea pig leukocyte lysates. This isolated material leads to antigen-dependent migration inhibition and thymidine uptake by nonimmune lymphoid cells. Tests of the "transfer factor" from guinea pigs immunized to either ovalbumin or bovine gamma-globulin demonstrated the donor specificity of the in vitro activity. The activity is susceptible to heat (56 degrees C), alkali (0.5 M sodium hydroxide), pronase, and phosphodiesterase. The pronase susceptibility is blocked by traysylol, a protease inhibitor; the phosphodiesterase susceptibility is not bocked by traysylol. The guinea pig factor was purified further by alkaline phosphatase treatment. Sephadex G-25 chromatography, and DEAE-cellulose chromatography. The final product, active in vitro, represents about 0.03% of the cellular material absorbing 260 nm light, and contains polymerized amines and phosphate. Gel electrophoresis of the fluram-reactive components suggests a limited heterogeneity of the DEAE-cellulose-purified material. These data are consistent with the active "transfer factor" molecule including both peptide and phosphate-containing components.
D000468:Alkalies; D000818:Animals; D007611:Aprotinin / Q000494:pharmacology; D002417:Cattle; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D000939:Epitopes; D006168:Guinea Pigs; D006358:Hot Temperature; D010047:Ovalbumin / Q000378:metabolism; D010727:Phosphoric Diester Hydrolases / Q000494:pharmacology; D011402:Pronase / Q000494:pharmacology; D014165:Transfer Factor / Q000276:immunology* / Q000302:isolation & purification; D005719:gamma-Globulins / Q000378:metabolism
D000468:Alkalies; D000939:Epitopes; D014165:Transfer Factor; D005719:gamma-Globulins; D010047:Ovalbumin; D007611:Aprotinin; D010727:Phosphoric Diester Hydrolases; D011402:Pronase