[The problem of preparing avid cytotoxic anti-B-lymphocyte serums].
There were anti-B-lymphocyte toxins in 40 samples (= 7.58%) from 527 examined sera of pregnant women and polytransfused persons without HLA cytotoxins and 84 samples (= 33.2%) from 253 anti-HLA sera. Of 49 specific anti-B sera 27 were suitable for typing B-lymphocyte antigens; with the help of 13 of these sera 5 specific antigens of B-lymphocytes called 1-5 could be determined. These 27 sera produced a positive cytotoxic reaction, mainly with the strength of +++ or ++. Attempts of absorbing HLA antibodies from anti-B lymphocyte sera led to unsatisfactory results. Sometimes the content of anti-B-lymphocyte toxins could also be diminished by a platelet absorption, in other cases the absorption was insufficient and had to be repeated therefore.
D000042:Absorption; D000961:Antilymphocyte Serum; D001402:B-Lymphocytes / Q000276:immunology*; D001792:Blood Platelets / Q000276:immunology; D001803:Blood Transfusion; D002469:Cell Separation; D003602:Cytotoxicity, Immunologic; D005260:Female; D006680:HLA Antigens; D006801:Humans; D007518:Isoantibodies / Q000302:isolation & purification; D011247:Pregnancy; D013601:T-Lymphocytes
D000961:Antilymphocyte Serum; D006680:HLA Antigens; D007518:Isoantibodies
Zum Problem der Gewinnung von aviden zytotoxischen Anti-B-Lymphozyten-Seren.