title: A system for the examination of tubercle bacilli and other mycobacteria.
abstract: Methods are described for the examination of mycobacteria cultured from clinical specimens. In the "screening" procedure used for new isolates tubercle bacilli are non-pigmented, do not grow at 25 degrees C and are sensitive to p-nitrobenzoic acid as well as normally to anti-tuberculosis drugs. Classification is extended when necessary by the use of four tests--temperature requirements, pigmentation, oxygen preference and Tween hydrolysis. These define 15 species or groups meeting the needs of clinical bacteriology. Drug-sensitivity tests are described which relate the end-points of titrations to the modal response of normal wild strains of M. tuberculosis. They are used not only as a guide to chemotherapy but also to support and amplify classification.
mesh_terms: D010131:Aminosalicylic Acid / Q000494:pharmacology; D003470:Culture Media; D007538:Isoniazid / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D009161:Mycobacterium / Q000145:classification* / Q000187:drug effects; D009163:Mycobacterium bovis / Q000145:classification; D009169:Mycobacterium tuberculosis / Q000145:classification*; D009579:Nitrobenzoates / Q000494:pharmacology; D010858:Pigmentation; D013696:Temperature; D013828:Thioacetazone / Q000494:pharmacology
chemical_list: D003470:Culture Media; D009579:Nitrobenzoates; D010131:Aminosalicylic Acid; D013828:Thioacetazone; D007538:Isoniazid