Immunofluorescence on resin-embedded material.
Some antigenic determinants can be preserved in tissues after plastic embedding. In the present study liver tissue was fixed with glutaraldehyde or paraformaldehyde, dehydrated with ethanol and toluene and embedded in araldite which was then polymerized at 37 degrees C. Immunofluorescence was performed on semi-thin sections etched with hydrogen peroxide. This procedure allows correlation with light microscopy (on the same stained semi-thin section) and with electron microscopy (on adjacent ultra-thin section). Good results were obtained with anti-nuclear, anti-mitochondrial, anti-microsomal and anti-alpha1-antitrypsin sera.
D000974:Antibodies, Antinuclear; D003584:Cytological Techniques; D000939:Epitopes; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D008099:Liver / Q000166:cytology; D008861:Microsomes / Q000276:immunology; D008930:Mitochondria, Liver / Q000276:immunology; D012117:Resins, Synthetic; D000515:alpha 1-Antitrypsin / Q000276:immunology
D000974:Antibodies, Antinuclear; D000939:Epitopes; D007106:Immune Sera; D012117:Resins, Synthetic; D000515:alpha 1-Antitrypsin