HLA-B specificities and w4, w6 specificities are on the same polypeptide.
The human alloantigenic specificities w4 and w6, which are products of a diallelic system genetically associated with the HLA-B locus, have been solubilized by papain digestion of membranes from the lymphoblastoid cell line, RPMI 4265. The w4 and w6 specificities copurified with the HLA-B locus products, HLA-B7 and HLA-B12. Sequential immunoprecipitation experiments were performed using alloantisera to HLA-B7, HLA-B12, w4 and w6, and a purified HLA-B7, B12, w4, w6 antigen pool labeled with 125I-Bolton-Hunter reagent. These experiments demonstrated directly that HLA-B7 and w6, which are genetically associated with each other, are different antigenic determinants on the same molecule, while HLA-B12 and w4, also genetically associated, are distinct antigenic determinants on a second molecule. Arguments are presented which suggest that the HLA-B determinants and w4, w6 determinants are in fact on the same polypeptide, and the genetic implications of the findings are discussed.
D000483:Alleles; D002460:Cell Line; D000939:Epitopes; D005796:Genes; D006680:HLA Antigens / Q000302:isolation & purification; D006649:Histocompatibility Antigens / Q000302:isolation & purification; D006801:Humans; D007519:Isoantigens / Q000302:isolation & purification; D010455:Peptides / Q000276:immunology*
D000939:Epitopes; D006680:HLA Antigens; D006649:Histocompatibility Antigens; D007519:Isoantigens; D010455:Peptides