Transmitter histochemistry of the rat olfactory bulb III. Autoradiographic localization of [3H]GABA.
The distribution of [3H]gamma-aminobutyric acid (GABA) labeled elements in rat olfactory bulb was studied by light and electron microscopic autoradiography. [3H]GABA was strongly taken up into glial cells and pericytes in all layers of the bulb. The neuronal uptake of [3H]GABA was mainly seen in certain types of nerve terminals. About one-third of the granule dendritic terminals, some nerve endings of short axon cells, and certain nerve endings of extrabulbar origin showed a strong labeling. Labeling was seen in a small population of the periglomerular, short axon and granule cell bodies. Most cell bodies of these 3 types as well as the mitral cells did not, however, accumulate any appreciable amo9nt of [3H]GABA. The labeling pattern seen after injection of [3H]glycine and [3H]leucine was clearly different from the pattern seen after [3H]GABA injection. The labeling was more uniformly distributed over the components of the neuropil with a considerably higher activity over certain cell somata such as the mitral cells. The present results demonstrate that neuronal uptake and accumulation of [3H]GABA occur into populations of olfactory bulb cells and processes, which from neurophysiological and/or immunohistochemical studies are supposed to use GABA as a neurotransmitter.
D000625:Aminooxyacetic Acid / Q000008:administration & dosage; D000818:Animals; D001345:Autoradiography; D001370:Axonal Transport; D001369:Axons / Q000648:ultrastructure; D001931:Brain Mapping; D003712:Dendrites / Q000648:ultrastructure; D007124:Immunoenzyme Techniques; D008297:Male; D009412:Nerve Fibers / Q000378:metabolism; D009457:Neuroglia / Q000378:metabolism; D009830:Olfactory Bulb / Q000033:anatomy & histology / Q000378:metabolism*; D009832:Olfactory Nerve / Q000378:metabolism; D011292:Premedication; D051381:Rats; D013569:Synapses / Q000378:metabolism / Q000648:ultrastructure; D005680:gamma-Aminobutyric Acid / Q000008:administration & dosage / Q000378:metabolism*
D000625:Aminooxyacetic Acid; D005680:gamma-Aminobutyric Acid