title: Genetic activity of bleomycin: differential effects on mitotic recombination and mutations in yeast.
abstract: The genetic effects of the antitumor antibiotic, bleomycin were studied in different strains of Saccharomyces cerevisiae. It was observed that the drug induced a high frequency of mitotic recombination and gene conversion. In contrast, it produced only a few mutations from adenine independence to adenine dependence and histidine dependence (a missense mutant) to histidine independence. In the strains carrying ochre-suppressible auxotrophic markers, no prototrophs were induced by this drug. The results indicating the specific activity of bleomycin are discussed and, in this connection, the usefulness of yeast as a test organism in mutagenicity screening is emphasized.
mesh_terms: D000483:Alleles; D001761:Bleomycin / Q000494:pharmacology*; D008938:Mitosis / Q000187:drug effects*; D009153:Mutagens; D011995:Recombination, Genetic / Q000187:drug effects; D012441:Saccharomyces cerevisiae / Q000187:drug effects
chemical_list: D009153:Mutagens; D001761:Bleomycin