title: Qualitative patterns of protein synthesis in the mouse oocyte.
abstract: Mouse oocytes were found to synthesize proteins actively at the germinal vesicle, metaphase I, metaphase II, and pronuclear (6 hours post-fertilization) stages. The qualitative pattern components being synthesized in vitro, as demonstrated using polyacrylamide gel electrophoresis, changed throughout maturation and fertilization. Oocytes were arrested at metaphase I by greater than 0.1 mug/ml cycloheximide or actinomycin D. The protein pattern in oocytes cultured in the presence of actinomycin D progresses to a metaphase II pattern in spite of the nuclear maturation arrest, indicating a dissociation between meiotic maturation and the changes in the pattern of proteins synthesized at different stages of maturation.
mesh_terms: D000818:Animals; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D005260:Female; D005306:Fertilization; D008297:Male; D008540:Meiosis; D051379:Mice; D009865:Oocytes / Q000166:cytology / Q000378:metabolism*; D010063:Ovum / Q000378:metabolism*; D014176:Protein Biosynthesis
chemical_list: D003609:Dactinomycin; D003513:Cycloheximide