Immune response to phosphorylcholine. I.V. Comparison of homologous and isologous anti-idiotypic antibody.
Homologous and isologous anti-idiotypic antibodies against the PC-binding BALB/c myeloma protein HOPC-8  were raised in A/He and BALB/c mice, respectively. Isologous and homologous anti-HOPC-8 serum suppressed the response to PC in vitro specifically. The antibodies were purified by using HOPC-8 immunoabsorbent. Purified isologous and homologous anti-idiotypic antibody were labeled with 125I and compared for Ig class composition and idiotype-binding specificity. Both kinds of anti-idiotypic antibodies were predominantly IgG1, were highly specific for the HOPC-8 idiotype, and had a smiliar affinities for the PC-binding site. These findings demonstrate that anti-HOPC-8 antibodies raised in A/He and BALB/c mice are very similar in their biologic and immunochemical poperties.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D000918:Antibody Specificity; D002794:Choline / Q000031:analogs & derivatives*; D000939:Epitopes; D005260:Female; D007074:Immunoglobulin G; D051379:Mice; D008815:Mice, Inbred Strains; D010767:Phosphorylcholine / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D000939:Epitopes; D007074:Immunoglobulin G; D010767:Phosphorylcholine; D002794:Choline