Noncytotoxic IgE-mediated release of histamine and serotonin from murine mastocytoma cells.
Cultured murine mastocytoma (AB-CBF1-MCT-1) cells were stimulated to release endogenous or incorporated histamine or serotonin by an IgE-mediated mechanisms without loss of viability. Stimulation was achieved by incubation of the cells with rat IgE-anti-IgE, rat IgE-anti-light chain, fluoresceinated rat IgE-anti-fluorescein, IgE-enriched mouse anti-ovalbumin-ovalbumin, or covalently linked dimers of rat IgE, at doses similar to those optimal for normal peritoneal mast cells. Active cell metabolism and Ca++ were required to obtain release. Despite the latter, no dose of the calcium ionophore, A23187, could be found which caused release without concomitant cytotoxicity. Phosphatidylserine did not enhance release.
D000818:Animals; D000906:Antibodies; D000888:Antibodies, Anti-Idiotypic; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D005452:Fluoresceins / Q000276:immunology; D006636:Histamine Release; D007073:Immunoglobulin E; D007147:Immunoglobulin Light Chains; D066298:In Vitro Techniques; D012515:Mast-Cell Sarcoma / Q000276:immunology*; D051379:Mice; D010047:Ovalbumin / Q000276:immunology; D051381:Rats; D012513:Sarcoma, Experimental / Q000276:immunology; D012701:Serotonin / Q000378:metabolism*
D000906:Antibodies; D000888:Antibodies, Anti-Idiotypic; D005452:Fluoresceins; D007147:Immunoglobulin Light Chains; D012701:Serotonin; D007073:Immunoglobulin E; D000001:Calcimycin; D010047:Ovalbumin; D002118:Calcium