Molecular hybridization probes for research in liver disease: studies with albumin cDNA.
A major avenue of our research has been to develop molecular probes (cDNAs) for studying the pathogenesis of liver disease (i.e., applied molecular pathophysiology). During the last 2-3 yr, we have developed and used molecular hybridization to study regulation of albumin synthesis in normal, protein-calorie deprived, uremic, and cirrhotic rat liver. Our current work is directed toward cloning the albumin gene to permit further analysis of albumin transcriptional and posttranscriptional control. Molecular hybridization, DNA cloning, related techniques can be utilized to study the function of virtually any gene. Incorporation of such advances in basic research into meaningful studies of liver disease is an exciting challenge to modern academic hepatology.
D000818:Animals; D001483:Base Sequence; D002474:Cell-Free System; D003001:Cloning, Molecular; D004247:DNA / Q000096:biosynthesis; D007676:Kidney Failure, Chronic / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008103:Liver Cirrhosis / Q000378:metabolism; D008107:Liver Diseases / Q000378:metabolism*; D008297:Male; D008722:Methods; D009693:Nucleic Acid Hybridization; D011132:Polyribosomes / Q000032:analysis; D011228:Prealbumin / Q000096:biosynthesis; D014176:Protein Biosynthesis; D012330:RNA, Double-Stranded / Q000096:biosynthesis; D012333:RNA, Messenger / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D051381:Rats; D012156:Reticulocytes / Q000378:metabolism; D012261:Ribonucleoproteins / Q000032:analysis; D012709:Serum Albumin / Q000096:biosynthesis*; D013217:Starvation / Q000378:metabolism; D013347:Subcellular Fractions; D014158:Transcription, Genetic
D011228:Prealbumin; D012330:RNA, Double-Stranded; D012333:RNA, Messenger; D012261:Ribonucleoproteins; D012709:Serum Albumin; D004247:DNA; D012194:RNA-Directed DNA Polymerase