B cell antigens of the HLA system: a simple serotyping technique based on non-cytotoxic anti-beta2-microglobulin reagents.
A modification of the NIH cytotoxicity test for recognizing B cell (D-associated) antigens and antibodies, when sera also contain anti-HLA(ABC) activity is described. The method is based on the observation that anti-beta2-microglobulin reagents are able to block lympholysis only when due to HLA(ABC) antigens.
D001402:B-Lymphocytes / Q000276:immunology*; D001609:Beta-Globulins / Q000276:immunology*; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods; D000939:Epitopes; D006680:HLA Antigens; D006649:Histocompatibility Antigens; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D007106:Immune Sera; D007140:Immunoglobulin Fab Fragments; D007202:Indicators and Reagents; D013696:Temperature; D001613:beta 2-Microglobulin / Q000276:immunology*
D001609:Beta-Globulins; D000939:Epitopes; D006680:HLA Antigens; D006649:Histocompatibility Antigens; D007106:Immune Sera; D007140:Immunoglobulin Fab Fragments; D007202:Indicators and Reagents; D001613:beta 2-Microglobulin