Development of an intravenous gamma-globulin with Fc activities. I. Preparation and characterization of S-sulfonated human gamma-globulin.
S-sulfonated gamma-globulin (S-GG) was prepared by treating gamma-globulin with sulfite and tetrathionate ions. About four interchain disulfide bonds were selectively cleaved to give S-sulfonate groups. Although the above treatment strongly suppresses anticomplementary activity and nonspecific skin reactivity, the resulting S-GG retains high antibody activity. Furthermore, S-GG was found to maintain satisfactory levels for prolonged periods in vivo. The physicochemical and antigenic analyses of S-GG suggest that the S-sulfonation induces structural modification only at restricted sites.
D003168:Complement Fixation Tests; D004220:Disulfides; D006801:Humans; D007141:Immunoglobulin Fc Fragments; D007275:Injections, Intravenous; D008968:Molecular Conformation; D013447:Sulfites; D013776:Tetrathionic Acid; D005719:gamma-Globulins / Q000008:administration & dosage / Q000302:isolation & purification*
D004220:Disulfides; D007141:Immunoglobulin Fc Fragments; D013447:Sulfites; D005719:gamma-Globulins; D013776:Tetrathionic Acid