title: Nucleotide sequence of the 3' terminal third of rabbit alpha-globin messenger RNA: comparison with human alpha-globin messenger RNA.
abstract: Rabbit and human alpha-globin complementary DNA was synthesized using the primer (dT10)dGdCdC hybridized to globin mRNA with reverse transcriptase, and sequenced using the plus and minus gel sequencing procedure (Sanger and Coulson, 1975; Brownlee and Cartwright, 1977). The complete 3' noncoding region, 89 nucleotides in length, and one third of the coding region of rabbit alpha-globin mRNA have thus been sequenced. These data are compared with the near complete 3' noncoding region sequence of human alpha-globin mRNA obtained in these and previous studies. The two mRNAs are approximately 80% homologous in their 3' noncoding regions, except that the human sequence has an insert of 24 nucleotides. A very similar insert in sequence has been described in human beta-globin mRNA (Proudfoot, 1977).
mesh_terms: D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000032:analysis / Q000096:biosynthesis; D003851:Deoxyribonucleases / Q000378:metabolism; D005815:Genetic Code; D005914:Globins; D006801:Humans; D009690:Nucleic Acid Conformation; D009711:Nucleotides / Q000032:analysis*; D009841:Oligonucleotides / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D011817:Rabbits
chemical_list: D009711:Nucleotides; D009841:Oligonucleotides; D012333:RNA, Messenger; D005914:Globins; D004247:DNA; D012194:RNA-Directed DNA Polymerase; D003851:Deoxyribonucleases