Stimulation of lymphocytes from patients with coeliac disease by a subfraction of gluten.
Peripheral-blood lymphocytes from patients with coeliac disease showed a marked increase in D.N.A. synthesis after incubation in vitro with a subfraction of gluten. Lymphocytes from control patients showed no increased D.N.A. synthesis under the same conditions. The possible use of this specific lymphocyte-stimulation assay as a screening test for coeliac disease is discussed.
D000293:Adolescent; D000328:Adult; D000368:Aged; D001284:Atrophy; D002446:Celiac Disease / Q000097:blood / Q000209:etiology*; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D005260:Female; D005983:Glutens / Q000633:toxicity*; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000150:complications / Q000175:diagnosis*; D066298:In Vitro Techniques; D007583:Jejunum / Q000473:pathology; D008214:Lymphocytes / Q000276:immunology* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D013268:Stimulation, Chemical; D013997:Time Factors
D005983:Glutens; D004247:DNA