DNA double-strand breaks and alkali-labile bonds produced by bleomycin.
Both in linear T2 DNA, analyyzed by velocity sedimentation, and in supercoiled Col EL DNA, analyzed by gel electrophoresis, the number of double-strand breaks produced by bleomycin was directly propotional to the number of single-strand breaks and was far greater than the number expected from random coincidence of single-strand breaks, suggesting that the bleomycin-induced double-strand breaks occur as an independent event. In Col EL DNA, at least twice as many single-strand breaks were found under alkaline assay conditions as were found under neutral conditions, showing the production of alkaline-labile bonds by bleomycin.
D001761:Bleomycin; D002499:Centrifugation, Density Gradient; D055598:Chemical Phenomena; D002621:Chemistry; D004247:DNA; D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D006863:Hydrogen-Ion Concentration; D007700:Kinetics
D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D001761:Bleomycin; D004247:DNA