[Isolation and characterization of beef thyroid giant RNA].
Total HMW-RNAs were prepared by three different methods (method with phenol, method with NaClO4, method without phenol using Ultrogel AcA 22 filtration). Giant RNAs were obtained in the void volume by filtration on Sepharose 2B. The giant RNAs/total HMW-RNA ratio is higher (6.77%) with the gel filtration method than with phenol or NaClO4 methods (1.41% and 1.00% respectively). The nucleotide composition of these RNAs is DNA-like and the sedimentation constants are approximately 70-100 S.
D000818:Animals; D002417:Cattle; D008970:Molecular Weight; D012313:RNA / Q000302:isolation & purification; D012265:Ribonucleotides / Q000032:analysis; D013961:Thyroid Gland / Q000032:analysis*
D012265:Ribonucleotides; D012313:RNA
Isolement et caractérisation des RNA géants de la glande thyroide de boeuf.