title: [Effect of dihydroxyacetone on metabolic parameters and survival of resuspended erythrocytes after storage at 25 degrees and 4 degrees].
abstract: Erythrocyte concentrates from CPD blood were resuspended after separating the leukocyte-thrombocyte layer and stored at 4 degrees C and 25 degrees C. The solutions for resuspension differed in the content of substrate, pH, condition of sterilization and the resuspension volume used, 1 or 4 volumes of cell concentrate to 1 volume of resuspension solution. At 4 degrees C, there was no effect of DHA on the the 2.3 bisphosphoglycerate content (P2G content). The decreased activity of triokinase at low temperature, phosphate deficiency and a partial disintegration of DHA during the autoclave as well as the difference of the commercial DHA preparations are discussed as underlying causes. At 25 degrees C DHA delayed the P2G decrease during storage. The enhanced triokinase activity above 15 degrees C is principally considered to be the cause for it. High rates of haemolysis, rapid ATP decrease, survival rates of 56% after a storage of three weeks for erythrocyte resuspensions with DHA in used compositions render a clinical application impossible at present. It could be well suitable as a substrate for resynthesizing P2G. An addition of ascorbate + nicotinamid + adenin at a storage temperature of 4 degrees C had no influence on the P2G content. A resuspension solution on the basis of xylitol with additions of sorbitol, bicarbonate, inorganic phosphate, pyruvate, adenine and guanosine showed the best properties in the content of P2G and ATP, lactate formation and survival rate during a storage at 4 degrees C.
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D001793:Blood Preservation; D002470:Cell Survival; D004098:Dihydroxyacetone / Q000494:pharmacology*; D004163:Diphosphoglyceric Acids / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects*; D005947:Glucose / Q000378:metabolism; D006461:Hemolysis; D006801:Humans; D007773:Lactates / Q000378:metabolism; D013696:Temperature; D014306:Trioses / Q000494:pharmacology*
chemical_list: D004163:Diphosphoglyceric Acids; D007773:Lactates; D014306:Trioses; D000255:Adenosine Triphosphate; D005947:Glucose; D004098:Dihydroxyacetone
vernacular_title: Einfluss von Dihydroxyazeton auf Stoffwechselparameter und Lebensfähigkeit resuspendierter Erythrozyten bei 25 degrees C- und 4 degrees C-Lagerung.