A rapid, simple, and reliable technique for preparation of antisera against idiotypes of homogeneous immunoglobulins.
One-step immunization of guinea pigs with homogeneous immunoglobulins partly purified by micro-agar electrophoresis represents a rapid and reliable microtechnique for the preparation of antisera against the idiotype of the homogeneous immunoglobulins. Testing of these antisera by immunodiffusion techniques requires agar plates containing 3% polyethyleneglycol 6000.
D000818:Animals; D004587:Electrophoresis, Agar Gel; D000939:Epitopes; D005838:Genotype; D006168:Guinea Pigs; D007106:Immune Sera / Q000302:isolation & purification*; D007114:Immunization; D007136:Immunoglobulins; D051379:Mice; D011092:Polyethylene Glycols; D011817:Rabbits; D013997:Time Factors
D000939:Epitopes; D007106:Immune Sera; D007136:Immunoglobulins; D011092:Polyethylene Glycols