A core polyprotein of murine leukemia virus on the surface of mouse leukemia cells.
A polypeptide of molecular weight approximately 75,000 daltons, p(75), was identified on the surface of AKR spontaneous leukemia cells by lactoperoxidase-catalyzed radio-iodination. This protein was shown by immunoprecipitation to have antigenic determinants of MuLV p30, p15, and p10, but not gp70, suggesting that p(75) represents a polyprotein composed of virion core components. As evidenced by studies on incorporation of radioactive glucosamine, p(75) is probably glycosylated. No p(75) was found on 2 month old AKR thymocytes, and only a small amount of p(75) was detectable of thymocytes from 4 month old animals. However, substantial quantities of p(75) could be found on thymocytes from 6 month old, yet still preleukemic mice.
D000367:Age Factors; D000818:Animals; D000956:Antigens, Viral; D002462:Cell Membrane / Q000032:analysis; D000939:Epitopes; D005944:Glucosamine / Q000378:metabolism; D009052:Leukemia Virus, Murine / Q000276:immunology; D007942:Leukemia, Experimental / Q000382:microbiology*; D051379:Mice; D008806:Mice, Inbred AKR; D013601:T-Lymphocytes / Q000382:microbiology* / Q000648:ultrastructure; D014764:Viral Proteins / Q000032:analysis* / Q000276:immunology / Q000378:metabolism
D000956:Antigens, Viral; D000939:Epitopes; D014764:Viral Proteins; D005944:Glucosamine