Specificity of DNA base release by bleomycin.
DNA was treated with bleomycin in the presence of Fe2+ and 2-mercaptoethanol under conditions where only a few percent of the bases were released. Release of all four bases was a linear function of bleomycin concentration, but the amount of thymine released was twice that of cytosine, 7 times that of adenine, and twelve times that of guanine. Unidentified minor products of thymine, of cytosine and of a purine were also released. Bromouracil did not sensitize DNA to bleomycin-induced breakage, and was released at the same rate as thymine.
D001761:Bleomycin; D055598:Chemical Phenomena; D002621:Chemistry; D004269:DNA, Bacterial; D004926:Escherichia coli; D007501:Iron; D007700:Kinetics; D008623:Mercaptoethanol; D011687:Purines / Q000032:analysis; D011743:Pyrimidines / Q000032:analysis
D004269:DNA, Bacterial; D011687:Purines; D011743:Pyrimidines; D001761:Bleomycin; D008623:Mercaptoethanol; D007501:Iron