[Polychromatic method of simultaneously demonstrating all the structural components of a peripheral nerve].
To study neuro-vessel relations in the nerve conductive system, a combined method revealing myelinated and non-myelinated nerve fibers and blood vessels was suggested. The method includes the following stages of material treatment: injection of the nerve blood vessels with chloroform emulsion of Paris blue (5--10 g of the dye in 100 g of the solvant); the straightened nerves about 5 mm long are fixed in 12% neutral formalin; etching in the solution of chromium solts; embedding in paraffin and preparation of thin sections (3--5 mcm); staining after Masson's method applied for collagen fiber staining) in the author's modification and mounting in balsam. In the preparations, the axons of the nerve fibers are seen blue--purple, myelin sheaths--light red, connective tissue elements--violet and blood vessels are seen as dark blue rings.
D000818:Animals; D004285:Dogs; D009413:Nerve Fibers, Myelinated / Q000166:cytology; D010525:Peripheral Nerves / Q000033:anatomy & histology* / Q000098:blood supply; D013194:Staining and Labeling / Q000379:methods; D014650:Vasa Nervorum / Q000033:anatomy & histology
Polikhromnyĭ sposob edinovremennogo vyiavleniia vsekh strukturnykh komponentov perifericheskogo nerva.