Specificity of antisera from Lewis rats immunized with encephalitogenic fragment 43-88 of guinea pig myelin basic protein.
Inbred Lewis rats were immunized with encephalitogenic fragment 43-88 of guinea pig myelin basic protein emulsified in complete Freund's adjuvant. After recovery from experimental allergic encephalomyelitis (EAE) the animals were given a booster immunization, bled, and the specificity of the individual anti-fragment antisera was examined by direct binding assays by using radioiodinated fragment 43-88 and peptides 43-67, 68-88, and 79-88. Competitive-binding experiments with these peptides in competition with labeled fragment 43-88 were also done. The results of these experiments indicated that the immunodominant region of fragments 43-88 was the carboxy-terminal half of the molecule. Individual antisera recognized different antigenic determinants within this region.
D000818:Animals; D000918:Antibody Specificity; D001665:Binding Sites; D001667:Binding, Competitive; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology; D000939:Epitopes; D006168:Guinea Pigs; D004676:Myelin Basic Protein / Q000276:immunology*; D010455:Peptides / Q000276:immunology; D051381:Rats; D011917:Rats, Inbred Lew
D000939:Epitopes; D004676:Myelin Basic Protein; D010455:Peptides