Cloning of an almost full-length chicken conalbumin double-stranded cDNA.
Chicken conalbumin double-stranded cDNA (con-dscDNA) was synthesized from a laying hen oviduct mRNA preparation enriched for conalbumin mRNA (con-mRNA). The dscDNA was inserted by blunt-end ligation into the Sal I site of plasmid pBR322 which had been repaired with DNA polymerase I to create Taq I sites on each side of the inserted fragment. After bacterial transformation, one hybrid recombinant, pBR322-con1, which contains the largest inserted dscDNA (about 2350 bp) was shown to hybridize specifically to the RNA which is translated into conalbumin. Electron microscopic examination of hybrid molecules between con-mRNA and pBR322-con1 DNA indicate that the inserted con-dscDNA is an almost full-length double-stranded transcript of conalbumin mRNA.
D000818:Animals; D001483:Base Sequence; D002645:Chickens; D003207:Conalbumin / Q000096:biosynthesis; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000096:biosynthesis*; D008854:Microscopy, Electron; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D010057:Oviducts / Q000378:metabolism*; D010957:Plasmids; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D012194:RNA-Directed DNA Polymerase
D004274:DNA, Recombinant; D012333:RNA, Messenger; D003207:Conalbumin; D012194:RNA-Directed DNA Polymerase; D004262:DNA Restriction Enzymes