Synthesis of procoagulant antihaemophilic factor in vitro.
The cells which produce the procoagulant part of antihaemophilic factor (factor VIII-C) in normal people have remained unknown. Normal blood leucocytes cultured in vitro with phytohaemagglutinin synthesised a procoagulant with the properties of factor VIII-C. The procoagulant was inhibited by human VIII-C inhibitor and itself absorbed human VIII-C inhibitor--properties which are regarded as specific for factor VIII-C. Unexpectedly, cultured leucocytes from haemophiliacs also synthesised the procoagulant in similar amounts. Possible explanations and implications of these findings are briefly considered.
D000925:Anticoagulants; D001780:Blood Coagulation Tests; D002478:Cells, Cultured; D005169:Factor VIII / Q000032:analysis; D005172:Factor XI / Q000096:biosynthesis* / Q000302:isolation & purification; D005173:Factor XI Deficiency / Q000097:blood; D006467:Hemophilia A / Q000097:blood; D006801:Humans; D066298:In Vitro Techniques; D007962:Leukocytes / Q000378:metabolism*; D008297:Male
D000925:Anticoagulants; D005169:Factor VIII; D005172:Factor XI