Measurement of antibody and antigen concentrations with a sodium sulphate glutaraldehyde technique.
Gamma-globulins can be crosslinked at low concentrations by simultaneous treatment with sodium sulphate and glutaraldehyde (SSG-method). This precipitate can be used for the assay of antibody or antigens. Examples are given for the measurement of (1) anti-dinitrophenyl antibody by reaction of the precipitate with 125I-dinitrophenylovalbumin, (2) IgA by inhibition of an anti-IgA precipitate with an 125I-labelled IgA myeloma protein, (3) idiotypic antibody by inhibition of the binding of a 125I-labelled myeloma protein by an anti-idiotype precipitate.
D000447:Aldehydes / Q000494:pharmacology*; D000818:Animals; D000906:Antibodies; D000941:Antigens; D001666:Binding Sites, Antibody; D002417:Cattle; D011232:Chemical Precipitation; D004136:Dinitrobenzenes / Q000276:immunology; D005976:Glutaral / Q000494:pharmacology*; D007118:Immunoassay / Q000379:methods; D007126:Immunoglobulin Allotypes; D051379:Mice; D010047:Ovalbumin / Q000276:immunology; D010954:Plasmacytoma / Q000276:immunology; D011817:Rabbits; D013431:Sulfates / Q000494:pharmacology*; D005719:gamma-Globulins
D000447:Aldehydes; D000906:Antibodies; D000941:Antigens; D004136:Dinitrobenzenes; D007126:Immunoglobulin Allotypes; D013431:Sulfates; D005719:gamma-Globulins; D010047:Ovalbumin; D005976:Glutaral