Role of I-J-subregion gene products in the regulation of macrophage-T-Lymphocyte interaction.
Pretreatment of splenic macrophages with anti-Ia serum specific for the various known I subregions and complement deleted the macrophages required for an in vitro antibody response to burro erythrocytes. Antibodies directed at the gene products of the I-J subregion, however, were unique in that they did not require the presence of complement. When macrophages and macrophage depleted cells were obtained from strains B10.A(3R) and B10.A(5R) differing only at the I-J subregion, they could not be mixed in culture for an effective response. Differences at other I subregions using alternative strain combinations resulted in an enhanced response. These results suggest that the I-J subregion has a critical role in macrophage-T cell interaction.
D000818:Animals; D000917:Antibody Formation; D000937:Antigen-Antibody Reactions; D000954:Antigens, Surface / Q000235:genetics; D000939:Epitopes; D004912:Erythrocytes / Q000276:immunology; D005802:Genes, MHC Class II; D007518:Isoantibodies; D007519:Isoantigens / Q000235:genetics*; D008211:Lymphocyte Cooperation; D008264:Macrophages / Q000276:immunology*; D008285:Major Histocompatibility Complex; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology*
D000954:Antigens, Surface; D000939:Epitopes; D007518:Isoantibodies; D007519:Isoantigens