Qualitative and quantitative evaluation of indirect immunofluorescent H-2 stain on tissue sections.
We have utilized H-2 specific antisera from congenic resistant donor recipient combinations to localize H-2 antigens in tissue sections using the indirect immunofluorescent technique. H-2 specific immunofluorescence is observed in spleen, kidney, and liver sections: however, especially with liver sections, there are cases in which the expected specific stain is weak or absent. In addition to microscopic evaluation by eye, we have employed a microscope attached photometer; recordings from this instrument differentiate positive from control stained spleen sections.
D000818:Animals; D000939:Epitopes; D005455:Fluorescent Antibody Technique; D006183:H-2 Antigens / Q000032:analysis*; D007106:Immune Sera; D007668:Kidney / Q000276:immunology*; D008099:Liver / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D013154:Spleen / Q000276:immunology*
D000939:Epitopes; D006183:H-2 Antigens; D007106:Immune Sera