[Formation of xanthomatous cells in vitro from the aortic media of the rat].
The foam cell is viewed as a specific component of the atherosclerotic plaque found in human or experimentally induced in the animal. A study using light microscopy (staining with Sudan III) and electron microscopy was performed on cell cultures derived from rat aortic media. Sudanophilic and electron transparent vacuoles were observed in vitro in 11 week cultures. The sudanophilic cells were either scattered or crowded in clusters; some of them were found in a mitotic phase. Different serums were applied to the cultures starting from the 6th week: either calf serum (continuing the previous treatment), or normocholesterolemic rat serum (NCRS) or hypercholesterolemic rat serum (HCRS). Sudanophilic cells were observed more frequently in the cultures on exposure to HCRS than to NCRS (p less than 0.05). Thus it was possible to induce the formation of foam cells in vitro in cultures of arterial tissue derived from the rat, which is known to be resistant to atherosclerosis.
D000818:Animals; D001011:Aorta / Q000473:pathology*; D001018:Aortic Diseases / Q000473:pathology*; D001161:Arteriosclerosis / Q000473:pathology; D002417:Cattle; D002478:Cells, Cultured; D002784:Cholesterol / Q000097:blood; D005487:Foam Cells / Q000473:pathology; D006937:Hypercholesterolemia / Q000097:blood; D066298:In Vitro Techniques; D051381:Rats; D013194:Staining and Labeling; D014617:Vacuoles / Q000648:ultrastructure; D014973:Xanthomatosis / Q000473:pathology*
D002784:Cholesterol
Formation de cellules xanthomateuses in vitro à partir de media aortique de rat