A rapid radioimmunoassay for human alpha-fetoprotein.
Our previous double antibody radioimmunoassay for human AFP was run at 4 degrees C and required 48 h for completion. A method is described which permits the assay to be performed within 5 h due to an increased incubation temperature and the use of polyethylene glycol to precipitate the antigen-antibody complex. The modified radioimmunoassay was standardized against an international reference preparation of human AFP. Characteristics of this assay are discussed.
D000818:Animals; D006041:Goats / Q000276:immunology; D006801:Humans; D008722:Methods; D011092:Polyethylene Glycols; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods; D013696:Temperature; D013997:Time Factors; D000509:alpha-Fetoproteins / Q000032:analysis*
D000509:alpha-Fetoproteins; D011092:Polyethylene Glycols