Antigenic analysis of human heart tissue--characterization of cardiac antigens.
The human heart antigens demonstrated in citric acid extracts by immunodiffusion were submitted to further analysis. Heart antigens were precipitated at 30% to 70% saturated ammonium sulphate. These fractions contained antigens reactive with rabbit antisera to human heart. The third fraction out of four proteins which were separated by Sephadex G-150 gel filtration, was reactive against rabbit antisera to human heart. The intensely stained proteins of both heart antigens were located at the nineth protein band out of proteins separated by polyacrylamide gel electrophoresis. This protein exhibited precipitin line against rabbit antisera to human heart by two-dimensional electrophoresis, and had mobility between that of serum alpha2 and beta1 globulin in immunoelectrophoresis.
D000941:Antigens / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes; D006801:Humans; D005779:Immunodiffusion; D009206:Myocardium / Q000276:immunology*
D000941:Antigens; D000939:Epitopes