title: The direct radioimmunoassay technique for the determination of human alpha1-fetoprotein.
abstract: The direct radioimmunoassay procedure for the detection of human alpha-fetoprotein is described. The technique requires 20 microliter of sample for a single estimation and includes ammonium sulfate precipitation. The whole assay can be completed in 18 hours. AFP concentrations ranging from 2 ng/m1 to 500 ng/m1 can be quantified with reproducibility sufficient for clinical as well as experimental purposes.
mesh_terms: D006801:Humans; D011863:Radioimmunoassay / Q000379:methods*; D013997:Time Factors; D000509:alpha-Fetoproteins / Q000032:analysis*
chemical_list: D000509:alpha-Fetoproteins