[Immuno-enzymatic estimation of steroids (author's transl)].
The authors present a reliable and sensitive estimation of progesterone using a conjugate progesterone IIalpha-hémimaleate-beta galactosidase to identify the immunological reaction, the antibodies being obtained from the rabbit by means of a progesterone 11alpha-hemisuccinate bovine serum albumin. The free and bound fractions are separated by addition of rabbit antigamma globulin antibodies bound on glass beads by glutaraldehyde. This method is applied to estimations of cortisol and oestrone.
D000888:Antibodies, Anti-Idiotypic; D004970:Estrone / Q000032:analysis; D006854:Hydrocortisone / Q000032:analysis; D007124:Immunoenzyme Techniques; D011374:Progesterone / Q000032:analysis*; D013256:Steroids / Q000032:analysis*; D001616:beta-Galactosidase; D005719:gamma-Globulins
D000888:Antibodies, Anti-Idiotypic; D013256:Steroids; D005719:gamma-Globulins; D004970:Estrone; D011374:Progesterone; D001616:beta-Galactosidase; D006854:Hydrocortisone
Dosage immuno-enzymatique des stéroïdes.