Isolation of amyloid P component (protein AP) from normal serum as a calcium-dependent binding protein.
The P component of amyloid (protein AP, pentagonal unit) has been isolated from normal serum by using its hitherto undescribed calcium-dependent affinity for agarose. The presence of P component in all forms of amyloid may be due to this calcium-dependent binding to certain polyanions.
D000682:Amyloid / Q000032:analysis* / Q000276:immunology; D001798:Blood Proteins / Q000302:isolation & purification*; D002118:Calcium; D002352:Carrier Proteins / Q000302:isolation & purification*; D003172:Complement C1 / Q000302:isolation & purification*; D003165:Complement System Proteins / Q000302:isolation & purification*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D008854:Microscopy, Electron; D011485:Protein Binding
D000682:Amyloid; D001798:Blood Proteins; D002352:Carrier Proteins; D003172:Complement C1; D003165:Complement System Proteins; D002118:Calcium