Immunoperoxidase localization of Sporothrix schenckii and Cryptococcus neoformans. Staining of tissue sections fixed in 4% formaldehyde solution and embedded in paraffin.
An indirect immunoperoxidase staining method has been successfully applied to 4% formaldehyde solution tissue sections fixed in and embedded in paraffin for the localization of Sporothrix schenckil and Cryptococcus neoformans without prior trypsinization of tissue sections. A comparison of this method with an analogous immunofluorescence staining technique has been made.
D003454:Cryptococcus / Q000302:isolation & purification*; D003455:Cryptococcus neoformans / Q000302:isolation & purification*; D005455:Fluorescent Antibody Technique; D005557:Formaldehyde; D006801:Humans; D007124:Immunoenzyme Techniques; D010232:Paraffin; D012867:Skin / Q000276:immunology; D013175:Sporothrix / Q000302:isolation & purification*; D013194:Staining and Labeling / Q000379:methods*
D005557:Formaldehyde; D010232:Paraffin