Influence of some aldehyde blocking agents on staining of depurinized DNA with cationic dyes.
Rat liver, spleen and Walker carcinosarcoma imprints were subjected to depurinizing Feulgen hydrolysis and then treated with blocking agents of aldehyde groups. Such blockators as sodium bisulfite and hydroxylamine which multiplay additionally anionic groups in DNA and intensify the reactions with cationic dyes, ensuring anisotropic staining. Hydrazine lowers the binding of carionic dyes to DNA, instead phenylhydrazine, completely blocks both aldehyde and phosphate groups. When the imprints were treated with 2.4-dinitrophenylhydrazine, aldehyde and phosphate groups of apurinic acid were blocked, and DNA staining by cationic dyes occurred only on account of nitrogroups of the blocking agents which have been used. The staining reaction of cationic dyes after the use of anionogenic blocking agents of aldehyde groups is prospective not only for revealing DNA but also for several other compounds with natural or potential aldo- and ketogroups. However the reaction with phenylhydrazine can serve as a staining without removal of DNA prior to staining as an optional procedure.
D000447:Aldehydes / Q000037:antagonists & inhibitors; D000818:Animals; D001080:Apurinic Acid; D002279:Carcinoma 256, Walker / Q000648:ultrastructure; D002467:Cell Nucleus / Q000032:analysis; D004396:Coloring Agents; D004247:DNA; D006651:Histocytochemistry; D007526:Isoelectric Point; D008099:Liver / Q000648:ultrastructure; D011119:Polynucleotides; D051381:Rats; D013154:Spleen / Q000648:ultrastructure; D013194:Staining and Labeling
D000447:Aldehydes; D004396:Coloring Agents; D011119:Polynucleotides; D001080:Apurinic Acid; D004247:DNA