[Use of 5-bromodeoxyuridine for determining the duration of cell cycle phases].
The method of differential staining of sister chromatids by means of 5-bromdesoxyuridine (BDU) was used to determine duration of the cellular cycle and its phases in a cell culture of Chinese hamster. The mitotic cycle lasted for 16 hrs, G1 for 5 hrs, S for 8 hrs and G2 for 3 hrs. These values do not differ from those obtained by the autoradiography. The given method permits quicker determination of the cellular cycle phases duration as compared with the autoradiography method.
D000818:Animals; D001973:Bromodeoxyuridine / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects*; D002478:Cells, Cultured; D006224:Cricetinae; D003412:Cricetulus; D004261:DNA Replication / Q000187:drug effects; D013194:Staining and Labeling / Q000379:methods; D013997:Time Factors
D001973:Bromodeoxyuridine
Ispol'zovanie 5-bromdezoksiuridina dlia opredeleniia prodolzhitel'nosti faz kletochnogo tsikla.