[Dynamic characteristics of the fluorescence of human peripheral blood neutrophilic polymorphonuclear leukocytes and lymphocytes intravitally fluorochrome-stained with acridine orange].
Neutrophil segmentonuclear leukocytes and lymphocytes of the human peripheral blood vitally stained with Acridine Orange (AO) in concentrations of 250 and 330 mcg/ml show different fluorescence dynamics. The number of neutrophil segmentonuclear granulocytes with green fluorescence of nuclei decreases, whereas the number of cells with red fluorescence of nuclei increases. As a criterion of this process, time T1/2 is taken during which the number of green-fluorescent cells decreases twofold. With AO concentrations of 250 and 330 mcg/ml, T1/2 is equal to 40 or 5 minutes, resp. The nuclei of lymphocytes within a 60 minutes observation show green fluorescence. This effect is likely to be due to structural-functional peculiarities of neutrophil segmentonuclear granulocytes and lymphocytes.
D000165:Acridine Orange / Q000008:administration & dosage*; D002467:Cell Nucleus / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005453:Fluorescence; D006801:Humans; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000187:drug effects*; D009504:Neutrophils / Q000187:drug effects*; D013194:Staining and Labeling / Q000379:methods; D013997:Time Factors
D000165:Acridine Orange
Osobennosti dinamiki fluorestsentsii neĭtrofil'nykh segmentoiadernykh leĭkotsitov i limfotsitov perifericheskoĭ krovi cheloveka, prizhiznenno fluorokhromirovannykh akridinovym oranzhevym.