The 3'-Terminal nucleotide sequence of encephalomyocarditis virus RNA.
Poly(A)-containing encephalomyocarditis virus RNA functions as an excellent template for cDNA synthesis in vitro with an RNA-dependent DNA polymerase in the presence of an oligothymidylate primer. Under appropriate conditions, discrete transcripts of increasing chain length were obtained, suitable for sequence analysis. A limited cDNA fragment of 36 nucleotides, primer (dT)10 included, was synthesized when dGTP was omitted from the reaction mixture and its primary structure was elucidated using direct DNA-sequencing methods. The complement corresponds to the 3' end of encephalomyocarditis RNA. The hexanucleotide (5'-3')(A-A-U-A-A-A) found in this sequence is also present in all 3' non-coding regions of poly(A)-containing eukaryotic mRNAs studied until now, in nearly identical positions relative to the poly(A) tail. The possible biological significance of this structural homology is discussed.
D001483:Base Sequence; D004686:Encephalomyocarditis virus / Q000032:analysis*; D007700:Kinetics; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000032:analysis; D011061:Poly A / Q000032:analysis; D012367:RNA, Viral / Q000302:isolation & purification; D012194:RNA-Directed DNA Polymerase
D009838:Oligodeoxyribonucleotides; D012367:RNA, Viral; D011061:Poly A; D012194:RNA-Directed DNA Polymerase