Acridine orange particles in cultured fibroblasts. A comparative study of macular corneal dystrophy, systemic mucopolysaccharidoses types I-H and II, and normal controls.
Vital staining with the fluorescent dye, acridine orange, was evaluated as a means of detecting abnormalities of lysosomes in cultivated fibroblasts of patients with macular corneal dystrophy and mucopolysaccharidoses types I-H (Hurler's syndrome) and type II (Hunter's syndrome). Multiple cultures were compared with normal fibroblasts using a "double-masked" design to exclude observer bias. Cells of patients with the mucopolysaccharidoses were easily and accurately separated from other fibroblasts. Contrary to a recent report, corneal fibroblasts of patients with macular corneal dystrophy were indistinguishable from control cells.
D000165:Acridine Orange; D000328:Adult; D003317:Corneal Dystrophies, Hereditary / Q000473:pathology*; D005069:Evaluation Studies as Topic; D005260:Female; D005347:Fibroblasts / Q000473:pathology*; D006801:Humans; D008247:Lysosomes / Q000473:pathology; D008875:Middle Aged; D008059:Mucopolysaccharidosis I / Q000473:pathology*; D016532:Mucopolysaccharidosis II / Q000473:pathology*; D013194:Staining and Labeling
D000165:Acridine Orange