Reverse transcriptase of foamy virus. Purification of the enzymes and immunological identification.
Reverse transcriptase from foamy virus, strain H4188 was estimated and purified. The enzyme has the following characteristics: 1. The reaction utilized preferentially oligo (dT) poly (rA) as a primer-template; however, the synthetic primer-template oligo (dT) poly (dA) could also be used to some extent. 2. The reaction utilized oligo (dG) poly (rC) as a primer-template with very low efficiency. 3. The crude virus preparation had a detectable endogenous reaction using the four deoxyribonucleotides for DNA polymerization. 4. The cation requirement for the enzyme reaction was much more biased for Mn++ than for Mg++ ions. 5. The molecular weight of the partially-purified enzyme was estimated to be about 80,000. Aggregates of 240,000 daltons were also seen. The activity of this enzyme was not inhibited by antisera against the reverse transcriptases of various type C RNA viruses, namely, feline endogenous leukemia virus, RD 114, Woolly simian sarcoma virus (SSV-1) and avian myeloblastosis virus (AMV). Antiserum against Rauscher leukemia virus (RLV) enzyme was marginally active against foamy virus enzyme, perhaps indicating a slight cross-reaction. The biochemical characteristics of foamy virus reverse transcriptase seemed to be very close to those of the type C RNA viruses, but the immunological reaction proved that the foamy virus reverse transcriptase was distinct from the others.
D000939:Epitopes; D008274:Magnesium / Q000378:metabolism; D008345:Manganese / Q000378:metabolism; D008970:Molecular Weight; D011067:Poly dA-dT / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D012190:Retroviridae / Q000201:enzymology*; D016092:Spumavirus / Q000201:enzymology* / Q000276:immunology; D013698:Templates, Genetic
D000939:Epitopes; D011067:Poly dA-dT; D008345:Manganese; D012194:RNA-Directed DNA Polymerase; D008274:Magnesium