Stereospecific opiate binding in human erythrocyte membranes and changes in heroin addicts.
Stereospecific opiate binding has been demonstrated in human erythrocyte membranes, having a Kd of 9-10(-9) M. In most respects the binding characteristics resemble those of synaptic membranes. These included the correlation of binding affinity and pharmacological potency of opiates; competition by naloxone; inhibition by Ca2+ and Na+; and sensitivity to phospholipases and trypsin. A comparison of stereospecific opiate binding in control human subjects and heroin addicts revealed a 43% increase in the addict group.
D000328:Adult; D001921:Brain; D002122:Calcium Chloride / Q000494:pharmacology; D003917:Dextrorphan / Q000494:pharmacology; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D006556:Heroin Dependence / Q000097:blood*; D006801:Humans; D004091:Hydromorphone / Q000378:metabolism*; D007700:Kinetics; D007981:Levorphanol / Q000494:pharmacology; D008297:Male; D009019:Morphinans / Q000494:pharmacology; D010740:Phospholipases / Q000494:pharmacology; D011957:Receptors, Opioid / Q000378:metabolism*; D012694:Serine / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D013237:Stereoisomerism; D013329:Structure-Activity Relationship; D013570:Synaptic Membranes / Q000187:drug effects / Q000378:metabolism; D014357:Trypsin / Q000494:pharmacology
D009019:Morphinans; D011957:Receptors, Opioid; D003917:Dextrorphan; D007981:Levorphanol; D012965:Sodium Chloride; D012694:Serine; D010740:Phospholipases; D014357:Trypsin; D002122:Calcium Chloride; D004091:Hydromorphone