title: Immunological characterization of the low-molecular-weight DNA binding protein of mouse mammary tumor virus.
abstract: p14, a low-molecular-weight MMTV protein previously identified as having DNA-binding properties and encoded by the gag region of the MMTV genome, was purified by affinity chromatography on DNA-sepharose. Immunological characterization of the purified protein showed that MMTV p14 shares no cross-reactivity with gp52, gp36 and p10, antigens associated with the MMTV envelope, nor with p27 antigen found in the virion core. Purified MMTV p14 did show cross-reactivity with purified intracytoplasmic A particles, supporting the concept that A particles are morphological precursors to MMTV cores. In addition, shared antigenic determinants between intracytoplasmic A particles and MMTV p27, p20 and p10 were demonstrated. MMTV p14 did not cross-react with the low-molecular-weight DNA-binding proteins of MuLV or of type-C or -D viruses of higher mammals.
mesh_terms: D000818:Animals; D000956:Antigens, Viral; D002460:Cell Line; D011232:Chemical Precipitation; D002846:Chromatography, Affinity; D003429:Cross Reactions; D003593:Cytoplasm / Q000276:immunology; D004279:DNA, Viral / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes; D005260:Female; D008324:Mammary Tumor Virus, Mouse / Q000276:immunology*; D051379:Mice; D008970:Molecular Weight; D011485:Protein Binding; D012328:RNA Viruses / Q000276:immunology; D011863:Radioimmunoassay; D014764:Viral Proteins / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D000956:Antigens, Viral; D004279:DNA, Viral; D000939:Epitopes; D014764:Viral Proteins