Specificity of human lymphocytes primed against allogeneic cells in vitro. II. Discrimination and cross-reactivity in repeated priming.
When studying the specificity of human lymphocytes primed in vitro against HLA-D determinants on allogeneic cells, it was found that specific restimulation of the primed cells 10 days after the first priming did not influence their discriminatory power compared to cells primed only once. Likewise, priming to one HLA-D determinant and repeated priming against another HLA-D determinant did not change the discrimination for the first priming antigen. Neither was there any increased relative reactivity to the second priming determinant. On simultaneous priming against two HLA-D determinants carried by two different stimulating cells, a good discrimination for both antigens was obtained compared to third party cells, but if the stimulations were separated by 24 h or more, the second priming was mainly without effect. Antigeneic competition is thought to be the mechanism involved in this early restriction of specificity.
D002478:Cells, Cultured; D003429:Cross Reactions; D000939:Epitopes; D006680:HLA Antigens; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*
D000939:Epitopes; D006680:HLA Antigens