Blood group activity of human sucrase from intestinal metaplasia.
Sucrases were purified from human small intestine and from areas of intestinal metaplasia of the stomach mucosa surrounding stomach cancers. The kinetic constants and pH activity profiles of enzyme preparations from the two sources were similar. No blood group activity of sucrase was detectable in preparations from three cases of intestinal metaplasia, but preparations from two other cases showed activity like that of the small intestine. These results indicate that sucrase from areas of intestinal metaplasia has similar enzymatic properties to those of enzyme from the small intestine, but that the antigenic sugar moiety of the enzyme associated with blood group activity varies.
D000017:ABO Blood-Group System; D000368:Aged; D000939:Epitopes; D005260:Female; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007421:Intestine, Small / Q000201:enzymology*; D007700:Kinetics; D008297:Male; D008679:Metaplasia / Q000201:enzymology; D008875:Middle Aged; D013274:Stomach Neoplasms / Q000201:enzymology*; D013393:Sucrase / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*
D000017:ABO Blood-Group System; D000939:Epitopes; D013393:Sucrase