Rapid axonal transport of tyrosine hydroxylase in rabbit sciatic nerves.
We compared the axonal transport of tyrosine hydroxylase with that of dopamine-beta-hydroxylase by means of an in vitro stop-flow technique. Proximal to a locally cooled nerve region, the activity of both enzymes increased linearly for 3 h. Internal standards of partially purified adrenal enzyme showed that the increased activity of tyrosine hydroxylase was not due to changes in activators or inhibitors. Furthermore, this increased activity represented increased V max, unaccompanied by changes in Km for tyrosine; probably, therefore, it reflected increased concentrations of the enzyme. From the rates of increase of enzyme activity, we calculated that, on the average, tyrosine hydroxylase was transported at 0.78 mm/h, and dopamine-beta-hydroxylase at 2.4 mm/h. Much less tyrosine hydroxylase than dopamine-beta-hydroxylase seemed free to move in the nerves, suggesting greater similarity between true transport velocities than between average velocities of these enzymes. When nerves were rewarmed after local cooling for 3 h, waves of accumulated enzyme activity moved distally. The wave of dopamine-beta-hydroxylase activity had a single peak moving at about 14 mm/h. The wave of tyrosine hydroxylase activity had a small component moving this fast, but more of it moved slower, at velocities down to 5 mm/h...
D000818:Animals; D001370:Axonal Transport; D003080:Cold Temperature; D004299:Dopamine beta-Hydroxylase / Q000378:metabolism; D007700:Kinetics; D009419:Nerve Tissue Proteins / Q000378:metabolism; D011817:Rabbits; D012584:Sciatic Nerve / Q000201:enzymology*; D013997:Time Factors; D014443:Tyrosine / Q000378:metabolism; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism*
D009419:Nerve Tissue Proteins; D014443:Tyrosine; D014446:Tyrosine 3-Monooxygenase; D004299:Dopamine beta-Hydroxylase