Kinetics of microcolonies of cultured mammalian cells after bleomycin treatment.
Cultured ascites hepatoma cells of the rat were treated with 400 to 900 R of gamma rays or 25 to 100 mug/ml of bleomycin for 30 min. The cells were followed up for 133 hr and the pedigrees were analyzed. Gamma rays and 2 low doses of bleomycin gave rise to typical reproductive death in generations 1 and 2 but 100 mug of the drug produced frequent interphase death. Natural death and induced death did not follow statistical randomness suggesting an existence of weak and sensitive clones in a population.
D001761:Bleomycin / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects* / Q000528:radiation effects; D002478:Cells, Cultured; D005720:Gamma Rays; D007700:Kinetics
D001761:Bleomycin