Dynamic redistribution of concanavalin A binding sites on isolated guinea pig keratinocytes.
Modifications in the distribution of the binding sites for concanavalin A (Con A) were studied on trypsin isolated living guinea pig keratinocytes. Fluorescein-labelled Con A was used and the in vitro procedure has included short-term cultures, experiments at 37 degrees C and 4 degrees C, the study of colchicine and vincaleucoblastine effects. It was possible to induce different patterns of staining corresponding to distinct redistribution of Con A binding sites; the distinct redistribution was correlated to the effects of Con A, colchicine and vincaleucoblastine. These findings demonstrated that the system used was appropriate to the study of some dynamic events on the keratinocytes membranes.
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects; D002469:Cell Separation; D003078:Colchicine / Q000494:pharmacology; D003208:Concanavalin A / Q000494:pharmacology; D004817:Epidermis / Q000032:analysis; D005453:Fluorescence; D006168:Guinea Pigs; D007633:Keratins / Q000096:biosynthesis*; D011952:Receptors, Concanavalin A / Q000032:analysis* / Q000187:drug effects; D011955:Receptors, Drug / Q000032:analysis*; D012867:Skin / Q000166:cytology*; D013194:Staining and Labeling; D013696:Temperature; D014357:Trypsin / Q000494:pharmacology; D014747:Vinblastine / Q000494:pharmacology
D011952:Receptors, Concanavalin A; D011955:Receptors, Drug; D003208:Concanavalin A; D014747:Vinblastine; D007633:Keratins; D014357:Trypsin; D003078:Colchicine