Staining of rickettsial antigens for microagglutination reaction.
Staining of antigens of R. prowazeki, R. canada and R. conori with hematoxylin-Harris for use in MAR was developed. Optimal results were obtained when rickettsial antigens were stained at 4 degrees C for 18 hours. Comparison of MAR with CFR has shown that MAR using stained rickettsial antigens is specific and as sensitive as CFR.
D000818:Animals; D000941:Antigens; D006168:Guinea Pigs / Q000276:immunology; D006386:Hemagglutination Tests; D006801:Humans; D051379:Mice / Q000276:immunology; D011817:Rabbits / Q000276:immunology; D012281:Rickettsia / Q000276:immunology*; D013045:Species Specificity; D013194:Staining and Labeling; D014438:Typhus, Epidemic Louse-Borne / Q000276:immunology / Q000382:microbiology
D000941:Antigens