Mean velocity of optically detected intraaxonal particles measured by a cross-correlation method.
A method which uses by the cross correlation of optical signals is described for the determination of the mean velocity of somatopetally moving particles within nerve fibers. The method was validated by simulation experiments and by comparing the results with those obtained by averaging collections of velocities of individual particles. The significant contribution of the method is that it allows objective and rapid serial evaluation of mean particle velocity within individual nerve fibers with good accuracy and precision. A series of results from normal myelinated nerve fibers from Xenopus laevis is presented. Considerable variation (up to 50%) in mean velocity was found between individual nerve fibers. The mean of all determinations indicates that the mean velocity of somatopetally moving particles in axons with diameters greater than 10mum is in the region of 1.14 mum/s at a temperature of 22-24 degrees C. The findings are compared with small collection of such determinations which have been reported in the literature.
D000818:Animals; D001001:Anura; D001370:Axonal Transport; D008722:Methods; D008959:Models, Neurological; D009413:Nerve Fibers, Myelinated / Q000502:physiology; D013997:Time Factors; D014981:Xenopus