[Kinetics of alpha phosphate turnover in the free nucleotides of isolated rabbit hearts during the incorporation of adenosine or uridine].
The isolated rabbit heart was perfused with tritium-labelled adenosine or uridine at constant concentration (respectively 10 and 2 micrometer) and specific activity. The transfer rates of the precursors into free nucleotides were obtained by calculating the uptake of isotope into the products. The rates of phosphorylation of the nucleotides were estimated by following the incorporation of 32PO4 3- into the alpha phosphate groups. The results obtained with the two isotopes show that the rate of labelling of the alpha phosphate groups is a good estimate of the turnover of nucleotides when the mechanism of synthesis involves phosphorylation of a riboside.
D000227:Adenine Nucleotides / Q000378:metabolism; D000241:Adenosine / Q000378:metabolism*; D000818:Animals; D009206:Myocardium / Q000378:metabolism*; D009711:Nucleotides / Q000096:biosynthesis*; D010710:Phosphates / Q000378:metabolism*; D011817:Rabbits; D014529:Uridine / Q000378:metabolism*
D000227:Adenine Nucleotides; D009711:Nucleotides; D010710:Phosphates; D000241:Adenosine; D014529:Uridine
Cinétique de renouvellement du phosphate alpha des nucléotides libres du coeur isolé de lapin, au cours d'une incorporation d'adénosine ou d'uridine.