title: Complement-mediated release of histamine from human basophils. III. Possible regulatory role of microtubules and microfilaments.
abstract: The release of histamine by normal human leukocytes (basophils) following in vitro challenge with activated complement (zymosan-treated serum) was previously reported. In this study, the effects of various pharmacologic agents on this release mechanism were compared with allergen-induced release of histamine. Colchicine and vinblastine antagonize the polymerization of tubulin to form microtubules, and both agents inhibited complement-and allergen-triggered release of histamine from basophils. Finally, treatment with cytochalasin B, a fungal product known to interfere with microfilament formatin, resulted in enhanced release of histamine from complement-treated basophils but no significant change in the percentage of histamine released from allergen-treated basophils. These findings suggest that microtubules and/or microfilaments are involved in complement-induced secretion of histamine by human basophils.
mesh_terms: D000485:Allergens; D001491:Basophils / Q000378:metabolism*; D003078:Colchicine / Q000494:pharmacology; D003182:Complement C5; D003571:Cytochalasin B / Q000494:pharmacology; D003599:Cytoskeleton / Q000502:physiology; D003903:Deuterium / Q000494:pharmacology; D006636:Histamine Release / Q000187:drug effects*; D006801:Humans; D008870:Microtubules / Q000502:physiology; D014747:Vinblastine / Q000494:pharmacology
chemical_list: D000485:Allergens; D003182:Complement C5; D003571:Cytochalasin B; D014747:Vinblastine; D003903:Deuterium; D003078:Colchicine