[The color rendition of microscopical stains by the dyes produced in reversal color films (author's transl)].
When comparing spectral and colorimetric data of colors of our natural environment with the data of microscopical stains, we observe considerable differences in spectral transmittance respectively reflectance. Furthermore, the spectral locus of these colors are situated at very different positions in the color space or in a chromaticity diagram. By this reason, we must anticipate that usual color reversal materials being optimized in the very first line for the reproduction of colors like foliage green, sky blue or earth, cannot render the hues of histological stains in a satisfying manner. To prove this assertion, the reproducible ranges of hues in the color space were calculated by means of the spectral densities of the dyes developed in the three layers of various color reversal films, and these results were then compared with the spectral data of some stains often applied for histology (H-E, Goldner, Azan etc.). By this method, we find that no color film examined can reproduce in a sufficient manner all these stains at the same time. The results can allow for color micrography the selection of a definite film for best possible color matching of a least one interesting range of color.
D003116:Color; D003124:Colorimetry; D004396:Coloring Agents; D004801:Eosine Yellowish-(YS); D010784:Photomicrography; D013053:Spectrophotometry; D013194:Staining and Labeling; D013843:Thiazines
D004396:Coloring Agents; D013843:Thiazines; D004801:Eosine Yellowish-(YS)
Die Wiedergabe mikroskopischer Färbungen durch die Farbstoffe der Umkehrfarbfilme.