Orcein staining of hepatitis B antigen (HBAg) in conventional paraffin sections of liver biopsies.
In an unselected group of 213 liver biopsies, paraffin sections were treated with an orcein staining method with the view to demonstrate HBAg. In 8 patients with positive HBAg serology, only 3 biopsies yielded positive orcein material in hepatocytes. A good correlation appears to exist between orcein positive material and the "ground glass" cytoplasm of liver cells.
D001706:Biopsy; D006509:Hepatitis B / Q000473:pathology; D006514:Hepatitis B Surface Antigens / Q000032:analysis*; D006801:Humans; D008099:Liver / Q000382:microbiology* / Q000473:pathology; D010078:Oxazines; D013194:Staining and Labeling
D006514:Hepatitis B Surface Antigens; D010078:Oxazines