[Enzyme-immunoassay of grass-pollen and mites antibodies in rabbit antisera on magnetic polyacrylamide agarose beads (author's transl)].
Complex antigens were coupled to polyacrylamide agarose beads which had been made magnetic with iron oxide. Those beads were then used in an enzyme-immunoassay for quantitation of specific antibodies to grass-pollen and mites in rabbit antisera. The results obtained by a direct or an inhibition technique proved the specificity of this assay towards homologous antigens. Moreover, cross-reactions between the various species of grass-pollen could be shown. One advantage of this simple method is that it may avoid use of radioactive compounds.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D003429:Cross Reactions; D000939:Epitopes; D007106:Immune Sera / Q000032:analysis*; D007124:Immunoenzyme Techniques; D008280:Magnetics; D008925:Mites / Q000276:immunology*; D006109:Poaceae / Q000276:immunology*; D011058:Pollen; D011134:Polysaccharides; D011817:Rabbits / Q000276:immunology; D012685:Sepharose
D000906:Antibodies; D000939:Epitopes; D007106:Immune Sera; D011134:Polysaccharides; D012685:Sepharose
Dosage enzymo-immunologique d'anticorps sériques anti-pollen de graminées et anti-acarien dans des immunsérums de lapin sur des perles de polyacrylamide agarose magnétiques.