Neutral proteases of human granulocytes. IV. Interaction between human granulocyte collagenase and plasma protease inhibitors.
Purified human granulocyte collagenase was inactivated by serum through the formation of complexes with alpha 1-antitrypsin and alpha 2-macroglobulin. A molar combining ratio of 1:1 was observed for each inhibitor. The affinity of alpha 2-macroglobulin was about 10 times that of alpha 1-antitrypsin for granulocyte collagenase. The molar concentration of alpha 1-antitrypsin in the blood exceeds that of alpha 2-macroglobulin by about 12 times, so that the inhibitors may be equally important for defence against granulocyte collagenase.
D006098:Granulocytes / Q000201:enzymology*; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D007962:Leukocytes / Q000201:enzymology*; D003012:Microbial Collagenase / Q000037:antagonists & inhibitors* / Q000097:blood; D010447:Peptide Hydrolases / Q000097:blood; D011480:Protease Inhibitors; D000515:alpha 1-Antitrypsin / Q000378:metabolism; D000511:alpha-Macroglobulins / Q000378:metabolism
D011480:Protease Inhibitors; D000515:alpha 1-Antitrypsin; D000511:alpha-Macroglobulins; D010447:Peptide Hydrolases; D003012:Microbial Collagenase