Measurement of acid phosphatase activity in various tissues and cultured cells.
Acid phosphatase activity has been measured in various tissues and in extracts of cultured cells. Cells were solubilized with NP40 at neutral pH, and thymolphthalein monophosphate was used as substrate. Reproducible assays were possible with a few as 10(6) cells.
D000135:Acid Phosphatase / Q000032:analysis*; D002460:Cell Line; D006801:Humans; D008297:Male; D009928:Organ Specificity; D011467:Prostate / Q000201:enzymology*; D011470:Prostatic Hyperplasia / Q000201:enzymology; D013644:Tartrates
D013644:Tartrates; D000135:Acid Phosphatase