title: Fluorescence microscopical visualization of rat mast cells by means of fluoresceine isothiocyanate labeled protamine sulfate.
abstract: Using fluoresceine isothiocyanate labeled protamine sulfate it is possible to stain rat mast cell fractions of isolated periotoneal cells in a selective way. The cells are not degranulated, though they have lost their histamine completely. The method shows the dissociation of the mediator release and the extrusion of the granules.
mesh_terms: D000818:Animals; D005260:Female; D005452:Fluoresceins; D006636:Histamine Release; D008407:Mast Cells / Q000378:metabolism / Q000648:ultrastructure*; D008856:Microscopy, Fluorescence; D011479:Protamines; D051381:Rats; D013861:Thiocyanates
chemical_list: D005452:Fluoresceins; D011479:Protamines; D013861:Thiocyanates