Induction of human antigen-specific and non-specific helper factors in vitro.
Human peripheral blood leucocytes, cultured for 4--6 days with the appropriate concentration of protein or synthetic polypeptide antigen, yield helper cells. These cells, after a further 24 hr incubation with the appropriate antigen release helper factors, which are of two types, antigen specific and non-specific; a similar situation to that described in the mouse. These factors are assayed by their effects on the plaque-forming cell response of mouse spleen cells cultures in vitro. The potential applications of this new assay for human T cell function are discussed.
D000328:Adult; D000418:Albumins / Q000276:immunology; D000818:Animals; D000941:Antigens / Q000276:immunology*; D002478:Cells, Cultured; D000939:Epitopes; D006241:Haptens; D006433:Hemocyanins / Q000276:immunology; D006462:Hemolytic Plaque Technique; D006801:Humans; D008211:Lymphocyte Cooperation; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008875:Middle Aged; D010455:Peptides / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000418:Albumins; D000941:Antigens; D000939:Epitopes; D006241:Haptens; D010455:Peptides; D006433:Hemocyanins