Determination of a new rapid plasmin inhibitor in human blood by means of a plasmin specific tripeptide substrate.
A method for determination of antiplasmin activity is presented. Plasmin and plasma are incubated, and the remaining plasmin activity is measured spectrophotometrically by means of the plasmin specific tripeptide substrate H-d-Val-l-Leu-l-Lys-p-nitroanilide. The method is simple, rapid and easily automatized. By the immunoadsorption technique, and with the aid of purified substances it is shown that the measured activity is mainly due to a new antiplasmin [2,4] and possibly to some extent to alpha1-antitrypsin and C1-esterase inhibitor have no antiplasmin activity in the method. Heparin and epsilonaminocaproic acid interfered with the assay.
D015119:Aminocaproic Acid / Q000494:pharmacology; D000991:Antithrombins / Q000378:metabolism / Q000494:pharmacology; D004797:Enzyme-Linked Immunosorbent Assay; D005341:Fibrinolysin / Q000037:antagonists & inhibitors* / Q000378:metabolism; D005342:Fibrinolysis; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D013379:Substrate Specificity; D000515:alpha 1-Antitrypsin / Q000378:metabolism; D000511:alpha-Macroglobulins / Q000378:metabolism
D000991:Antithrombins; D000515:alpha 1-Antitrypsin; D000511:alpha-Macroglobulins; D006493:Heparin; D005341:Fibrinolysin; D015119:Aminocaproic Acid