Association of mouse major histocompatibility and Rauscher murine leukaemia virus envelope glycoprotein antigens on leukaemia cells and their recognition by syngeneic virus-immune-cytotoxic T-lymphocytes.
Physical association was measured between MLV gp70, the envelope glycoprotein of Rauscher murine leukaemia virus (R-MLV), and serologically defined H-2 antigens on the surface of R-MLV transformed C57BL/6 (H-2DbKb) mouse leukaemia cells (RBL-5A). Capping and patching with antisera against H-2Db caused specific co-capping and co-patching of R-MLV gp70 antigens as seen by fluorescence microscopy. Despite the physical proximity of R-MLV gp70 and H-2Db antigens, high titre alphaR-MLV gp70 sera had no effect in blocking syngeneic T-lymphocyte mediated cytolysis of RBL-2A cells whereas alphaH-2Db sera were effective.
D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity; D000956:Antigens, Viral; D002462:Cell Membrane / Q000276:immunology; D000939:Epitopes; D005260:Female; D006023:Glycoproteins / Q000276:immunology*; D006183:H-2 Antigens; D006389:Hemagglutinins, Viral / Q000276:immunology; D007106:Immune Sera; D007942:Leukemia, Experimental / Q000276:immunology*; D008285:Major Histocompatibility Complex; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D011925:Rauscher Virus / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology* / Q000637:transplantation; D014185:Transplantation, Isogeneic
D000956:Antigens, Viral; D000939:Epitopes; D006023:Glycoproteins; D006183:H-2 Antigens; D006389:Hemagglutinins, Viral; D007106:Immune Sera