title: Lens antigen development in chick embryos studied in vivo and in vitro by immunofluorescence.
abstract: Lens antigens, detected by immunofluorescence using rabbit antiserum against adult chick lens, appear in the chick embryo at stage 16. When eye rudiments are cultured in vitro, antigens developed; but they did not when optic cups were cultured but for a few cases. Isolated presumptive lens ectoderm from stage 4 did not develop antigens when cultured, but such ectoderm from stages 7--9 developed lens antigens and also showed lens structures. Stage 4 ectoderm could be induced to lens antigen development by alcohol-killed cups from stages 9--13. The experimental system can be used for in vitro studies on lens induction.
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D004475:Ectoderm / Q000166:cytology / Q000276:immunology; D000939:Epitopes; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D007908:Lens, Crystalline / Q000166:cytology / Q000196:embryology* / Q000276:immunology
chemical_list: D000939:Epitopes; D007106:Immune Sera