Shared determinants between virus-infected and trinitrophenyl-conjugated H-2-identical target cells detected in cell-mediated lympholysis.
Infection of H-2-identical mice with either lymphocytic choriomeningitis (LCM) virus, vaccinia virus, or paramyxo (Sendai) virus resulted in the generation of specifically sensitized cytotoxic T lymphocytes (CTL). CTL generated in vitro against 2,4,6-trinitrophenyl (TNP)-conjugated syngeneic stimulator cells were specifically cytotoxic for TNP-conjugated H-2K (D) region identical targets. Both LCM and vaccinia-induced CTL, however, were found to be strongly cytotoxic towards TNP-conjugated, H-2K(D) region-identical target cells. In contrast, Sendai virus-induced CTL did not lyse TNP-conjugated, syngeneic target cells. Inhibition experiments using cold targets suggested that shared antigenic determinants can be detected on either LCM virus-infected and TNP-conjugated targets, which are not present on the cell surface of normal target cells.
D000818:Animals; D002478:Cells, Cultured; D003080:Cold Temperature; D003429:Cross Reactions; D003601:Cytotoxicity Tests, Immunologic; D000939:Epitopes; D006649:Histocompatibility Antigens; D008217:Lymphocytic choriomeningitis virus / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D009578:Nitrobenzenes / Q000494:pharmacology*; D010222:Parainfluenza Virus 1, Human / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D014301:Trinitrobenzenes / Q000494:pharmacology*; D014616:Vaccinia virus / Q000276:immunology*
D000939:Epitopes; D006649:Histocompatibility Antigens; D009578:Nitrobenzenes; D014301:Trinitrobenzenes