Horseradish peroxidase as a retrogradely-transported, detailed dendritic marker.
A method is described that produces detailed 'Golgi-like' staining in neurons following retrograde transport of horseradish peroxidase (HRP). Of 4 commercially available HRP preparations, which were compared for their ability to produce solid neuronal staining in certain regions of the rabbit thalamus 48 h after striatal injection, two preparations (Miles Laboratories and Serva Biochemical) were found to be most efficient. When dimethylsulfoxide (DMSO) was used as the solvent for the injected HRP, a significant increase in the number of solidly labeled neurons in the thalamus was realized. Approximately 2% DMSO produced the greatest increase when compared with water as the solvent. Dendritic detail visualized by this method is shown to compare favorably with that produced by Golgi staining.
D000818:Animals; D001370:Axonal Transport; D002540:Cerebral Cortex / Q000166:cytology; D003342:Corpus Striatum / Q000166:cytology; D003712:Dendrites / Q000166:cytology* / Q000187:drug effects; D005260:Female; D006652:Histological Techniques; D006735:Horseradish Peroxidase / Q000378:metabolism; D010544:Peroxidases; D011817:Rabbits; D013378:Substantia Nigra / Q000166:cytology; D013788:Thalamus / Q000166:cytology
D006735:Horseradish Peroxidase; D010544:Peroxidases