[Use in histological technic of naphtol AS-D chloroacetate for demonstration of granulocyte series elements].
The authors report their experience of a histo-enzymatic method, using Naphtol AS-D Chloroacetate as a substrate, for the specific demonstration of elements of the granulocyte series, in histological sections, after formol fixation and mounting in paraffin wax. This simple and reliable staining technique offers the possibility of very rapid diagnostic orientation in haematological conditions.
D001854:Bone Marrow Cells; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D003937:Diagnosis, Differential; D006098:Granulocytes / Q000201:enzymology*; D006652:Histological Techniques; D006801:Humans; D007951:Leukemia, Myeloid / Q000175:diagnosis*; D008099:Liver / Q000166:cytology; D008198:Lymph Nodes / Q000166:cytology; D009283:Naphthol AS D Esterase / Q000378:metabolism*; D009284:Naphthols; D013154:Spleen / Q000166:cytology; D013194:Staining and Labeling / Q000379:methods*
D009284:Naphthols; D009283:Naphthol AS D Esterase; D002265:Carboxylic Ester Hydrolases
Utilisation en technique histologique du Naphtol AS-D chloroacétate pour la mise en évidence des éléments de la lignée granulocytaire.