Mechanism of conjugation and recombination in bacteria. XX, Recombination DNA synthesis during mating in Escherichia coli K-12.
In crosses of dnaA recipients with HfrH at restrictive temperature a functional recombinant structure and viable recombinants were formed at a nearly normal rate, whereas recombination in crosses with HfrC was markedly inhibited. Recombinational DNA synthesis, as determined by 3H-thymine incorporation, was found in dnaA merozygotes from HfrH crosses. The recombinational synthesis was completed shortly after the end of mating. The replication of recombinant chromosomes in dnaA Lac+ recombinants started 120 minutes after interruption of mating, and at the restrictive temperature was sensitive to acridine orange, thus pointing to the presence of integrated F factor in the recombinant chromosome.
D000165:Acridine Orange / Q000494:pharmacology; D003227:Conjugation, Genetic; D004261:DNA Replication / Q000187:drug effects; D004269:DNA, Bacterial / Q000096:biosynthesis; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D005144:F Factor; D009154:Mutation; D011995:Recombination, Genetic; D013997:Time Factors
D004269:DNA, Bacterial; D004259:DNA-Directed DNA Polymerase; D000165:Acridine Orange