Serological identification of five HLA-D associated (Ia-like) determinants.
Five antisera raised within HLA-A and -B compatible, HLA-D disparate combinations were reactive in a modified NIH microcytotoxicity test with B lymphocytes, but not with T lymphocytes from the immunizing donor, as well as with B lymphocytes from most or all donors sharing his immunizing HLA-D phenotype. Four antisera recognized structures closely associated with the HLA-D determinants Dw2, Dw3, Dw4 and LD 108. One serum had a broad reactivity pattern including Dw3, Dw6 and some unknown specificity(ies). In population and family studies, these B lymphocyte antigens behaved as if they were governed by one genetic locus in the B-D region of the HLA complex. We conclude that the antisera produced by this method recognize Ia-like antigens identical to or very closely associated with the HLA-D determinants.
D001402:B-Lymphocytes / Q000276:immunology; D003165:Complement System Proteins; D003601:Cytotoxicity Tests, Immunologic; D000939:Epitopes; D005787:Gene Frequency; D006680:HLA Antigens; D006649:Histocompatibility Antigens; D006650:Histocompatibility Testing; D006720:Homozygote; D006801:Humans; D007106:Immune Sera; D007114:Immunization; D008856:Microscopy, Fluorescence; D010641:Phenotype; D013601:T-Lymphocytes / Q000276:immunology
D000939:Epitopes; D006680:HLA Antigens; D006649:Histocompatibility Antigens; D007106:Immune Sera; D003165:Complement System Proteins