title: Release of arylsulfatase A but not B from rat mast cells by noncytolytic secretory stimuli.
abstract: The net percentage of release of arylsulfatase activity from purified rat mast cells induced by rabbit anti-rat F(ab')2 was consistently only about 1/3 that of histamine. Isoelectric focusing of the released and residual arylsulfatase activities demonstrated specific release of the A type without B and a net percentage of immunologic release of arylsulfatase A equivalent to that of histamine. When the net percentage of histamine and arylsulfatase A release were nearly maximal (88 and 76%) in response to the calcium ionophore A23187, specific release of arylsulfatase B did not occur. Thus, arylsulfatase A and not B was associated with the secretory granule released from the rat mast cell by reversed anaphylaxis or the calcium ionophore. In contrast, subcellular fractionation of water-lysed mast cells yielded arylsulfatase B with the heparin- and chymase-containing granule fraction and arylsulfatase A in the aqueous fraction comprised of cell sap and granule water eluate. It may be that arylsulfatase B resides in a minor second granule, whereas arylsulfatase A is loosely associated with the predominant secretory granule of the rat mast cell.
mesh_terms: D000118:Acetylglucosaminidase; D000818:Animals; D001192:Arylsulfatases / Q000378:metabolism*; D000001:Calcimycin / Q000494:pharmacology; D003594:Cytoplasmic Granules / Q000378:metabolism; D005260:Female; D006493:Heparin; D006632:Histamine; D006636:Histamine Release; D007140:Immunoglobulin Fab Fragments; D007525:Isoelectric Focusing; D008297:Male; D008407:Mast Cells / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D013429:Sulfatases / Q000378:metabolism*
chemical_list: D007140:Immunoglobulin Fab Fragments; D000001:Calcimycin; D006632:Histamine; D006493:Heparin; D013429:Sulfatases; D001192:Arylsulfatases; D000118:Acetylglucosaminidase