A competitive-inhibition radioimmunoassay for influenza virus envelope antigens.
A double-antibody competitive-inhibition radioimmunoassay for influenza virus envelope antigens is described. A viral antigen preparation from influenza A virus recombinant MRC11 [antigenically identical to A/Port Chalmers/1/73 (H3N2)] consisting of haemagglutinin and neuraminidase was labelled with radioiodine. Rabbit antisera were allowed to react with the labelled antigen and the resultant antigen-antibody complexes were precipitated with the appropriate antiglobulin. The competitive-inhibition radioimmunoassay was very sensitive in elucidation of differences even among closely related influenza virus strains. Attempts have been made to eliminate neuraminidase from radioimmunoprecipitation in order to obtain a competitive-inhibition radioimmunoassay system for haemagglutinin alone.
D000956:Antigens, Viral / Q000032:analysis*; D000939:Epitopes; D006389:Hemagglutinins, Viral / Q000032:analysis*; D009980:Influenza A virus / Q000276:immunology*; D009439:Neuraminidase / Q000032:analysis / Q000276:immunology*; D011863:Radioimmunoassay / Q000379:methods*
D000956:Antigens, Viral; D000939:Epitopes; D006389:Hemagglutinins, Viral; D009439:Neuraminidase