Differential silver carbonate staining of sister chromatids in BrdU-substituted chromsomes.
Differential staining of sister chromatids in BrdU-substituted human chromosomes is demonstrated by an ammoniacal silver carbonate procedure. With this method the chromosomes exhibit a subchromatid structure. Because proteolytic treatment indicated that the silver carbonate binds the chromosome proteins, changes of these components may be inferred in the BrdU-substituted chromosomes. Sister chromatid exchanges could be identified.
D001973:Bromodeoxyuridine; D002842:Chromatids; D002877:Chromosomes, Human; D006801:Humans; D008214:Lymphocytes; D011485:Protein Binding; D012834:Silver; D013194:Staining and Labeling
D012834:Silver; D001973:Bromodeoxyuridine