Flow cytometry: long-term storage of propidium iodide/citrate-stained material.
Single cell suspensions stained by the propidium iodide/hypotonic citrate method for DNA content analysis by flow cytometry can be mixed with an equal amount of 70% alcohol for long-term storage and shipping without introduction of artifacts or loss of fluorescence.
D002478:Cells, Cultured; D002951:Citrates; D004247:DNA / Q000032:analysis*; D004273:DNA, Neoplasm / Q000032:analysis; D004996:Ethidium / Q000031:analogs & derivatives; D006801:Humans; D007945:Leukemia, Lymphoid / Q000032:analysis; D008214:Lymphocytes / Q000032:analysis; D008722:Methods; D013194:Staining and Labeling / Q000379:methods*; D013997:Time Factors; D014021:Tissue Preservation / Q000379:methods*
D002951:Citrates; D004273:DNA, Neoplasm; D004247:DNA; D004996:Ethidium