Feulgen type staining with Hoffmann's violet-SO2 under exposure to UV rays.
The paper contains an account of the use of Hoffmann's violet-SO2 under exposure to UV rays during staining acid-hydrolysed DNA of mammalian tissue nuclei. Preparations stained with Hoffmann's violet-SO2 without exposure to UV rays reveal extremely pale violet nuclei but when stained under the influence of UV rays show a considerably faster reaction resulting in a very much deeper staining of the nuclei. Sections after staining with this dye-reagent require n-butanol as differentiating reagent. Possible interpretation for the increase in staining ability of this dye-reagent under exposure to UV rays has been elucidated and the reason for considering the reaction as Feulgen type has been discussed.
D000311:Adrenal Glands / Q000648:ultrastructure; D000818:Animals; D002467:Cell Nucleus / Q000032:analysis; D004247:DNA / Q000032:analysis*; D008099:Liver / Q000648:ultrastructure; D008297:Male; D051381:Rats; D013194:Staining and Labeling / Q000379:methods*; D013737:Testis / Q000648:ultrastructure; D014466:Ultraviolet Rays
D004247:DNA