Analysis of thy-1 variants of murine lymphoma cells.
Cells obtained from a radiation-induced T-cell lymphoma of BALB/c mice, RLmale-1, were adapted to long-term tissue culture. Clones of cells were obtained in soft agar and a clonally derived population studied for the frequency of mutation in the expression of the surface antigen Thy-1.2 (OC3H) by immunoselection. The mutation rate was 1.05-1.50 X 10(-6) per cell per generation. Antigenic and structural analysis of prototype positive and negative clones demonstrated clear differences between them.
D000818:Animals; D002999:Clone Cells; D000939:Epitopes; D005260:Female; D014644:Genetic Variation; D007519:Isoantigens; D008223:Lymphoma / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009374:Neoplasms, Experimental / Q000235:genetics / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000939:Epitopes; D007519:Isoantigens