title: [Affinity alkylation of E. coli RNA-dependent DNA polymerase with TTP gamma-amidate derivatives].
abstract: TTP gamma-benzylamidates are shown to act as competitive inhibitors of poly(dT) synthesis catalyzed by E. coli RNA-dependent DNA polymerase. The KM value for TTP as well as KI values for the gamma-analogues have been determined. TTP gamma-4-(N-2-chloroethyl-N-methyl-amino)benzylamidate is shown to be an effective affinity reagent for this enzyme.
mesh_terms: D000345:Affinity Labels; D000478:Alkylation; D004926:Escherichia coli / Q000201:enzymology*; D007700:Kinetics; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*; D013942:Thymine Nucleotides / Q000494:pharmacology*
chemical_list: D000345:Affinity Labels; D013942:Thymine Nucleotides; D012194:RNA-Directed DNA Polymerase
vernacular_title: Affinnoe alkilirovanie RNK-zavisimoĭ DNK-polimerazy E. coli proizvodnym gamma-amida TTR.