Electrophoretic evaluation of different hormonal receptor sites in the prostate during anti-hormonal treatment and in response to hormones.
Physical separations of different receptor entities by gradient ultracentrifugation or by separations based on ionic strength are interfered with, either by contamination with non-specific binding sites or they are irrelevant because of the low yield of sites after processing. Electrophoretic separation may give a better resolution because of the different nett charges on SHBG and the specific receptors. Isoelectric microfocussing allows the additional differentiation of sites based on the individual isoelectric points of each entity. Using this method, differentiation of at least three different sites in prostate tissue has been achieved.
D006801:Humans; D066298:In Vitro Techniques; D007525:Isoelectric Focusing; D008297:Male; D011470:Prostatic Hyperplasia / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000032:analysis*; D011956:Receptors, Cell Surface / Q000302:isolation & purification*; D012738:Sex Hormone-Binding Globulin / Q000302:isolation & purification
D011956:Receptors, Cell Surface; D012738:Sex Hormone-Binding Globulin