Differential Giemsa staining of sister chromatids after extraction with acids.
Chromosomes of Chinese hamster strain cells were air-dried on slides after BrdU substitution for two or three rounds of replication. The preparations were treated with 20% PCA at 55 degrees C for 20-30 min, or 5N HCl at 55 degrees C for 15-20 min. After staining with Giemsa, unifilarly BrdU-substituted chromatids stained faintly and bifilarly substituted chromatids stained darkly. Such a pattern of sister chromatid differential staining was confirmed by the examination of metaphase cells grown with BrdU for three rounds of replication.
D001399:Azure Stains; D002460:Cell Line; D002842:Chromatids; D006851:Hydrochloric Acid; D010472:Perchlorates; D010640:Phenothiazines; D013194:Staining and Labeling / Q000379:methods*
D001399:Azure Stains; D010472:Perchlorates; D010640:Phenothiazines; D006851:Hydrochloric Acid