Role of divalent ion complex formation in pyran--inhibition of nucleic acid biosynthesis.
The degree of inhibition of mammalian DNA-dependent RNA polymerases I and II and Moloney leukemia virus RNA-dependent DNA polymerase by pyran copolymer was dependent on the concentration of the divalent cation cofactor in the reaction mixture. Inhibition was completely blocked by an excess of divalent cations. It was concluded that pyran inhibited these enzymes by complexing with the essential divalent cation cofactor.
D000818:Animals; D002413:Cations, Divalent / Q000494:pharmacology; D002614:Chelating Agents; D055598:Chemical Phenomena; D002621:Chemistry; D012321:DNA-Directed RNA Polymerases / Q000037:antagonists & inhibitors*; D004492:Edetic Acid / Q000494:pharmacology; D066298:In Vitro Techniques; D051379:Mice; D008979:Moloney murine leukemia virus / Q000201:enzymology; D011108:Polymers / Q000494:pharmacology*; D011713:Pyran Copolymer / Q000494:pharmacology*; D012318:RNA Polymerase I / Q000037:antagonists & inhibitors*; D012319:RNA Polymerase II / Q000037:antagonists & inhibitors*; D018894:Reverse Transcriptase Inhibitors
D002413:Cations, Divalent; D002614:Chelating Agents; D011108:Polymers; D018894:Reverse Transcriptase Inhibitors; D011713:Pyran Copolymer; D004492:Edetic Acid; D012319:RNA Polymerase II; D012321:DNA-Directed RNA Polymerases; D012318:RNA Polymerase I