Variable opacity of glycogen in routine electron micrographs.
Glycogen in nerve terminals from the reticular zone of frog muscle was identified by transmission electron microscopy of both periodic acid-thiosemicarbazide-silverproteinate treated and UAc-PbCi-stained serial sections. A variable appearance of glycogen in the uranylacetate-lead citrate-stained nerve terminals was seen and is related to the preparative procedure. The study indicates the necessity of cytochemical identification for the assessment of glycogen organization in cells.
D000818:Animals; D001001:Anura; D006003:Glycogen / Q000378:metabolism; D008854:Microscopy, Electron; D009411:Nerve Endings / Q000378:metabolism / Q000648:ultrastructure*; D009469:Neuromuscular Junction / Q000648:ultrastructure; D011894:Rana pipiens; D013194:Staining and Labeling
D006003:Glycogen