Sensitivity and background staining of the unlabeled antibody enzyme (PAP) method. new approaches to the investigation of the effects of non-immune serum, primary antiserum concentration and pronase digestion using formalin fixed paraffin embedded material.
The effect of primary antibody concentration, non-immune serum, and pronase digestion on the stainability of immunoglobulin containing cells (plasma cells) and background stainability was investigated using the peroxidase-antiperoxidase (PAP) method and antigens consisting of formalin fixed paraffin embedded maxillary mucosa, colonic mucosa, inflammatory synovia and soft-tissue plasmocytoma. The effect of primary anti-serum concentration and non-immune serum on cell and background stainability was smaller, but pronase digestion clearly improved positive stainability and reduced background stainability.
D000818:Animals; D003106:Colon / Q000166:cytology; D004306:Dose-Response Relationship, Immunologic; D005557:Formaldehyde; D006801:Humans; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D007136:Immunoglobulins / Q000302:isolation & purification; D007413:Intestinal Mucosa / Q000166:cytology; D009061:Mouth Mucosa / Q000166:cytology; D010232:Paraffin; D010950:Plasma Cells / Q000276:immunology; D010954:Plasmacytoma / Q000276:immunology; D011402:Pronase; D013194:Staining and Labeling / Q000379:methods*; D013583:Synovial Membrane / Q000166:cytology
D007106:Immune Sera; D007136:Immunoglobulins; D005557:Formaldehyde; D010232:Paraffin; D011402:Pronase