title: The effect of the protein content of diluents on peak height in rocket immunoelectrophoresis.
abstract: We have investigated the effect of different diluents in the quantitation of proteins by rocket immunoelectrophoresis. alpha2-Macroglobulin produced taller peaks as the protein content of the diluent fell to zero, and the dilution was increased. A maximum increase in height of 25-30% was recorded when saline was used as the diluent compared to a sample diluted in sheep serum. Albumin and transferrin showed similar, but less significant effects. Samples and standards should therefore contain approximately equal concentrations of protein for maximum accuracy.
mesh_terms: D006801:Humans; D007122:Immunoelectrophoresis / Q000379:methods*; D007202:Indicators and Reagents; D011506:Proteins; D012709:Serum Albumin / Q000032:analysis; D014168:Transferrin / Q000032:analysis; D000511:alpha-Macroglobulins / Q000032:analysis
chemical_list: D007202:Indicators and Reagents; D011506:Proteins; D012709:Serum Albumin; D014168:Transferrin; D000511:alpha-Macroglobulins