Nature of the antigenic complex recognized by T lymphocytes. VI. The effect of anti-TNP antibody on T cell responses to TNP-conjugated macrophages.
In this paper we examined the effect of anti-TNP antibody on guinea pig T cell proliferation in response to TNP-modified macrophages in vitro. The addition of anti-TNP to TNP-modified macrophages immediately after conjugation inhibited their ability to stimulate TNP-specific T cell proliferation. This inhibition appeared to be specific for the TNP response since anti-TNP had no effect on the ability of TNP-modified macrophages pulsed with either PPD or TNP-Ova to stimulate efficient PPD or Ova T cell responses. On the other hand, anti-TNP had no effect on the TNP-specific response to TNP-modified macrophages that had been cultured overnight before addition to primed T cells or to macrophages which had been pulsed with TNP-Ova. We also demonstrated that the same TNP-specific T cell subpopulation responds to both freshly TNP-modified macrophages and overnight cultured TNP-modified macrophages. These results suggest that the relevant TNP-determinants recognized by T cells are not exposed on the macrophage surface and raise the possibility that macrophages must process membrane-conjugated TNP to create the immunogen recognized by T cells.
D000818:Animals; D000906:Antibodies; D000961:Antilymphocyte Serum / Q000494:pharmacology; D001667:Binding, Competitive; D002478:Cells, Cultured; D000939:Epitopes; D006168:Guinea Pigs; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology*; D009578:Nitrobenzenes / Q000276:immunology*; D010047:Ovalbumin / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D013997:Time Factors; D014301:Trinitrobenzenes / Q000276:immunology*; D014373:Tuberculin
D000906:Antibodies; D000961:Antilymphocyte Serum; D000939:Epitopes; D009578:Nitrobenzenes; D014301:Trinitrobenzenes; D014373:Tuberculin; D010047:Ovalbumin