Feulgen type staining of mammalian tissues with Dahlia-SO2.
The use of the basic dye, Dahlia, which belongs to triphenylmethane group but without a primary amino group in its molecule has been described as useful in the staining of aldehyde groups of acid hydrolysed DNA in tissue sections following the conventional Feulgen procedure. Dahlia-SO2 prepared with sodium hydrosulphite is highly suitable when used at pH 4-0 to 5-0. The absorption characteristics of the stained nuclei indicate on the peak of maximum absorption at 560 nm, whereas, that of the aqueous dye solution is at 590 nm.
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D004396:Coloring Agents; D004247:DNA / Q000032:analysis*; D005069:Evaluation Studies as Topic; D007668:Kidney / Q000648:ultrastructure; D008099:Liver / Q000648:ultrastructure; D051381:Rats; D013194:Staining and Labeling / Q000379:methods; D014320:Triphenylmethyl Compounds
D004396:Coloring Agents; D014320:Triphenylmethyl Compounds; D004247:DNA