Gas-liquid chromatographic determination of kepone in field-collected avian tissues and eggs.
A procedure is described for determining Kepone (decachlorooctahydro-1,3,4-metheno-2H-cyclobuta [cd] pentalene-2-one) residues in avian egg, liver, and tissue. Samples were extracted with benzene-isopropanol, and the extract was cleaned up with fuming H2SO4-concentrated H2SO4. Kepone was separated from organochlorine pesticides and polychlorinated biphenyls on a Florisil column and analyzed by electron capture gas-liquid chromatography (GLC). The average recovery from spiked tissues was 86%. The analyses performed on 14 bald eagle carcasses and livers, 3 bald eagle eggs, and 14 osprey eggs show measurable levels which indicate that Kepone accumulates in the tissues of fish-eating birds. Residues were confirmed by GLC-mass spectrometry.
D000818:Animals; D001717:Birds / Q000378:metabolism*; D007631:Chlordecone / Q000032:analysis*; D002849:Chromatography, Gas / Q000379:methods; D004531:Eggs / Q000032:analysis*; D007306:Insecticides / Q000032:analysis*; D010573:Pesticide Residues / Q000032:analysis
D007306:Insecticides; D010573:Pesticide Residues; D007631:Chlordecone