[Demonstration of Q bands on mitotic chromosomes of the amphibian urodele Pleurodeles poireti Gervais, after staining with quinacrine mustard. Comparison with Pleurodeles waltii].
Preparations of metaphase chromosomes of the amphibian urodele Pleurodeles poireti were obtained by squashing cells from tailbuds of ten days old embryos which had been in 50% acetic acid. The Q-bands karyotype is described. Frequently the induced secondary constrictions exhibit a bright fluorescence, as do the centromeres, except for that of chromosome XII. In contrast, satellites on chromosomes III, IV, and XI exhibit little fluorescence. This pattern is compared with that in Pleurodeles waltlii. Differences are observable in centromeres of chromosomes III and XII and the proximal part of the long arm of chromosome VII, differences which can be used as chromosome marker in case of hybridization.
D000663:Amphibians / Q000235:genetics*; D000818:Animals; D007621:Karyotyping / Q000379:methods; D008938:Mitosis; D011797:Quinacrine Mustard; D013194:Staining and Labeling
D011797:Quinacrine Mustard
Mise en évidence de zones Q sur les chromosomes mitotiques de l'amphibien urodèle Pleurodeles poireti Gervais, après coloration par la moutarde de quinacrine. Comparaison avec Pleurodeles waltlii.