title: 33258 Hoechst enhancement of the photosensitivity of bromodeoxyuridine-substituted cells.
abstract: Cells that have incorporated 5-bromodeoxyuridine (BrdU) into their DNA become extremely sensitive to photoinduced killing after treatment with the dye, 33258 Hoechst. Under appropriate conditions the dye increases the efficiency of killing by a factor of approximately 10(4). This enhanced photosensitivity suggests that the combination of BrdU, dye, and light can be used to select conditional lethal mutants.
mesh_terms: D001562:Benzimidazoles / Q000494:pharmacology*; D006690:Bisbenzimidazole / Q000494:pharmacology*; D001973:Bromodeoxyuridine / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival; D004359:Drug Therapy, Combination; D008027:Light; D009154:Mutation; D010777:Photochemistry
chemical_list: D001562:Benzimidazoles; D001973:Bromodeoxyuridine; D006690:Bisbenzimidazole