title: Transfer of hydrophilic molecules by placenta and yolk sac of the guinea pig.
abstract: Prenatal transfer of hydrophilic materials was studied in chronic guinea pig preparations of ca. 30-65 days gestation. In most preparations the vitelline (yolk sac placenta) circulation of one of the fetuses was ligated between 1 and 20 days before the experiment. Fetal and maternal serum protein concentrations were recorded as a function of the number of days after ligation. Prenatal transfer of hydrophilic materials of 60-5,500 daltons molecular wt was consistent with diffusion, with some evidence of steric restriction of the larger molecules. There was no difference between operated and sham-operated or control fetuses. However, the transfer of radiolabeled homologous gamma globulin ceased after ligation of the yolk sac vessels. The transfer in control fetuses accounted for most of the prenatal requirements of this protein, as calculated from the increases in fetal weight and serum gamma globulin concentrations as a function of gestational age. There was evidence of a nonspecific suppression of yolk sac function by surgery but no evidence of fetal catabolism of gamma globulin.
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000378:metabolism; D002463:Cell Membrane Permeability; D005026:Ethylene Glycols / Q000378:metabolism; D005260:Female; D006168:Guinea Pigs; D007122:Immunoelectrophoresis; D008026:Ligation; D008353:Mannitol / Q000378:metabolism; D008431:Maternal-Fetal Exchange; D008433:Mathematics; D008970:Molecular Weight; D010920:Placenta / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000378:metabolism; D011247:Pregnancy; D014508:Urea / Q000378:metabolism; D015017:Yolk Sac / Q000098:blood supply / Q000378:metabolism*; D005719:gamma-Globulins / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D001798:Blood Proteins; D005026:Ethylene Glycols; D005719:gamma-Globulins; D008353:Mannitol; D011092:Polyethylene Glycols; D014508:Urea