Transitory increase in chromosomal DNA (Fleulgen) during floral differentiation in Rhoeo discolor.
Scanning cytophotometric measurements on 3200 telophase and 1700 interphase nuclei (Feulgen-stained) in vegetative and reproductive buds of Rhoeo discolor revealed a transitory increase in staining intensity in more than half of the cells in early differentiating floral buds. The differences between the vegetative and floral nuclei are significant at the 0.001 level of probability and highly reproducible, independent of the type of hydrolysis used. We suggest that the different Feulgen extinction values reflect different nuclear DNA amounts, because methodical errors can fairly be excluded. The occurrence of an extra DNA (control DNA) of the kind of the floral DNA' detected by Wardell and Skoog (1973) and Wardell (1976) in tobacco is discussed.
D002875:Chromosomes / Q000032:analysis*; D004247:DNA / Q000032:analysis*; D010783:Photometry; D010944:Plants / Q000648:ultrastructure*; D013194:Staining and Labeling
D004247:DNA