Restriction in the immune response to flagellar proteins in inbred mice.
Anti-idiotypic sera prepared in both AL/N mice and rabbits identify specificities common to the IgA MOPC 467 myeloma protein and day-7 affinity-absorbed antibodies to Salmonella milwaukee polymerized flagellin (S. mil-POL) raised in BALB/c and C57BL/Ka mice. Isoelectric focusing (IEF) of reduced and alkylated BALB/c anti-S. mil-POL gave a banding pattern of L and H-chains identical to MOPC 467. C57BL/Ka anti-S. mil-POL had a similar L-chain pattern but a different H-chain pattern. Both BALB/c and C57BL/Ka contain predominantly IgA. The IEF patterns in the two strains are consistent with a monoclonal response at day 7.
D000818:Animals; D000907:Antibodies, Bacterial; D001426:Bacterial Proteins / Q000276:immunology*; D000939:Epitopes; D005260:Female; D005408:Flagellin / Q000276:immunology*; D005838:Genotype; D007143:Immunoglobulin Heavy Chains / Q000032:analysis; D007147:Immunoglobulin Light Chains; D007525:Isoelectric Focusing; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009194:Myeloma Proteins / Q000276:immunology; D011817:Rabbits; D012475:Salmonella / Q000276:immunology*
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D000939:Epitopes; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D009194:Myeloma Proteins; D005408:Flagellin