Dependence of efficiency of DNA transfer and recombinat formation on cell cycle of Hfr in Escherichia coli K-12.
The efficiency of DNA transfer during the Hfr cell cycle, studied with the use of 3H-thymidine, is the highest in the first half of the cycle. The efficiency of recombinant formation in the Hfr cell cycle demonstrates a similar periodicity only when the ratio of 1 Hfr cell to 8 or more F- cells in mating mixture is maintained. The absence of such changes in the number of recombinants during the cell cycle of a donor with relative excess of Hfr cells seems to be caused by limitation of the number of recombinants by the competent recipent cell fraction.
D002455:Cell Division; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000378:metabolism*; D004926:Escherichia coli / Q000502:physiology*; D005144:F Factor; D007700:Kinetics; D011995:Recombination, Genetic
D004269:DNA, Bacterial