Frozen-thawed mononuclear cells as a source of B lymphocytes for antibody screening.
B lymphocytes were isolated from fresh blood and frozen-thawed lymphocyte preparations from the same donors. A panel of 55 human antisera, previously found to react with human peripheral blood B lymphocytes in cytotoxicity assays, was screened against these B-cell preparations. There was essentially no difference in the reactions of B-cell antisera with fresh B lymphocytes when compared with frozen-thawed cells. The percentage recovery of B cells from the fresh and frozen preparations was not significantly different.
D001402:B-Lymphocytes / Q000276:immunology*; D002469:Cell Separation; D000939:Epitopes; D005615:Freezing; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D008214:Lymphocytes; D009000:Monocytes; D014021:Tissue Preservation
D000939:Epitopes