Development of radioimmunoassay for pregnancy-specific beta1-globulin and its measurement in serum of patients with trophoblastic and non-trophoblastic tumours.
Levels of human pregnancy-specific or trophoblast-specific beta1-globulin (TBG) were measured by double-antibody radioimmunoassay (RIA) in 103 patients with trophoblastic tumours and in 114 patients with a variety of non-trophoblastic tumours. The sensitivity of RIA for TBG was about 1 ng per ml. Twenty healthy adult females and males had levels below 1 ng per ml. Using the RIA for measurement of TBG in sera which were selected from immunodiffusion-TBG-negative samples, we detected elevated TBG levels in 76.7% of cases with trophoblastic tumours and in 15% of different cases with non-trophoblastic malignancies. The diagnostic and prognostic significance of the measurement of TBG levels by RIA in patients with trophoblastic tumours is discussed.
D000328:Adult; D001609:Beta-Globulins / Q000032:analysis*; D002822:Choriocarcinoma / Q000097:blood; D005260:Female; D005326:Fetal Proteins / Q000032:analysis*; D006801:Humans; D008297:Male; D009363:Neoplasm Proteins / Q000032:analysis* / Q000097:blood; D009369:Neoplasms / Q000097:blood; D011247:Pregnancy; D011863:Radioimmunoassay; D014328:Trophoblastic Neoplasms / Q000097:blood* / Q000175:diagnosis; D014594:Uterine Neoplasms / Q000097:blood*
D001609:Beta-Globulins; D005326:Fetal Proteins; D009363:Neoplasm Proteins