title: Identification of mouse bone marrow colony growth in agar-cultures by the nitroblue tetrazolium dye.
abstract: Identification of mouse bone marrow colony growth in soft agar cultures has been realized by layering of the plates with nitroblue tetrazolium at the concentrations of 1 mg/ml. Granulocyte-macrophage colonies appear dark-blue coloured on the yellow surrounding medium and can be fixed, stored at 4 degrees C and counted few days later. This method allows the cultures to be assessed macroscopically and makes a better enumeration of small clusters even if spread and consisting of only 30-40 cells.
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D006098:Granulocytes; D008264:Macrophages; D051379:Mice; D009580:Nitroblue Tetrazolium; D013194:Staining and Labeling / Q000379:methods*; D013696:Temperature; D013778:Tetrazolium Salts
chemical_list: D013778:Tetrazolium Salts; D009580:Nitroblue Tetrazolium