Solubilization and partial characterization of a tumor-associated transplantation antigen of the guinea pig L2C leukemia.
A tumor-associated transplantation antigen (TATA) from guinea pig L2C leukemia cells was solubilized by different methods. It was found that the 3 M KCl extraction yielded the most immunogenic TATA of L2C cells. Immunization of normal strain 2 guinea pigs with this extract in complete Freund's adjuvant gave complete protection against a subsequent challenge with tumor cells. Further fractionation of the KCl extract of L2C cells by Sephadex G-200 chromatography suggested that the immunogenic activity was present in the fraction containing materials with estimated m.w. of less than 20,000 daltons.
D000645:Ammonium Sulfate / Q000494:pharmacology; D000818:Animals; D000951:Antigens, Neoplasm; D002457:Cell Extracts; D002850:Chromatography, Gel; D000939:Epitopes; D006168:Guinea Pigs; D007942:Leukemia, Experimental / Q000276:immunology*; D009368:Neoplasm Transplantation; D010206:Papain / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000494:pharmacology; D011189:Potassium Chloride / Q000494:pharmacology; D012995:Solubility
D000951:Antigens, Neoplasm; D002457:Cell Extracts; D000939:Epitopes; D011092:Polyethylene Glycols; D011189:Potassium Chloride; D010206:Papain; D000645:Ammonium Sulfate