Macrophage-dependent response of immune human T lymphocytes to PPD in vitro. Influence of HLA-D histocompatibility.
The proliferative response of immune human T lymphocytes to low concentrations of purified protein derivative (PPD) in vitro was found to require the presence of macrophages. By coculturing different combinations of immune T lymphocytes and allogeneic macrophages with PPD in low concentration, optimal stimulation (that is, as efficient as the autologous combinations) was shown to require HLA-D identity between the macrophages and responding T cells. Identity for HLA-A and -B appeared to be of less or no importance. It is concluded that membrane structures encoded by HLA-D or closely linked loci are involved in the human macrophage/T-lymphocyte interaction in the proliferative response to PPD, much in the same way as the Ia antigens of rodents.
D002455:Cell Division / Q000187:drug effects; D000939:Epitopes; D006680:HLA Antigens; D006649:Histocompatibility Antigens; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects*; D008264:Macrophages / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014373:Tuberculin / Q000494:pharmacology*
D000939:Epitopes; D006680:HLA Antigens; D006649:Histocompatibility Antigens; D014373:Tuberculin