Absorption and elution of anti-DLA-A and B antibodies with acetone-dried dog spleen powder.
Acetone-dried powders were prepared from the spleens of DLA-serotyped dogs and assayed for their specific absorptive properties for anti-DLA-A and B antisera. The specific activity of two different antisera (anti-DLA-A9 and anti-DLA-B13) was readily absorbed with acetone-dried powders prepared from the spleens of dogs of the corresponding DLA types. This specific activity was recovered by the elution of the powder used for the serum absorption. The same method was used to narrow the specificity of a highly polyspecific antiserum. The comparison of the absorption properties of acetone-dried spleen powder versus fresh spleen cells shows that the treatment with acetone does not modify qualitatively and quantitatively the DLA specificities.
D000096:Acetone; D000818:Animals; D000918:Antibody Specificity; D003601:Cytotoxicity Tests, Immunologic; D003890:Desiccation / Q000379:methods*; D004285:Dogs; D000939:Epitopes; D005260:Female; D006649:Histocompatibility Antigens / Q000032:analysis*; D007163:Immunosorbent Techniques; D007518:Isoantibodies / Q000302:isolation & purification*; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D010641:Phenotype; D013154:Spleen / Q000276:immunology*
D000939:Epitopes; D006649:Histocompatibility Antigens; D007518:Isoantibodies; D000096:Acetone