[Rapid method of combined staining of myelinated fibers and brain cells].
A modification of the method of combined staining of myelin fibers and nerve tissue cells (Klüver and Barrera) is offered. Deparaffinised sections are stained for 1--2 hours in 0.01% acid alcohol solution of luxol blue fast, differentiated in 0.1% sodium tetraborate solution, and poststained with cresyl violet fast according to Nissl. The proposed method considerably shortens the time of staining and simplifies the process of differentiation of stained myelin fibers. The prescription for staining of the brain tissue sections silver-impregnated by the method of Holmes with luxol blue fast and cresyl violet is presented.
D001921:Brain / Q000473:pathology*; D006801:Humans; D008722:Methods; D009413:Nerve Fibers, Myelinated / Q000473:pathology*; D013194:Staining and Labeling
Uskorennyĭ metod kombinirovannogo okrashivaniia mielinovykh volokon i kletok mozga.