Double minute chromosomes and the homogeneously staining regions in chromosomes of a human neuroblastoma cell line.
Four human neuroblastoma cell lines were studied by chromosome banding techniques. All of the lines contained a marker chromosome with a long nonbanding homogeneously staining region (HSR). The HSR-containing chromosome differed in each line. One line contained two classes of cells: one with an HST marker chromosome and the other with double minute chromosomes. Each cell had one of these abnormalities; no cell had both. The presence of two additional chromosomal markers in all cells of this line indicates a common origin. These observations suggest that the double minute chromosomes are derived from the HSR.
D002460:Cell Line; D002869:Chromosome Aberrations; D002877:Chromosomes, Human / Q000648:ultrastructure*; D006801:Humans; D007621:Karyotyping; D009447:Neuroblastoma / Q000648:ultrastructure*; D013194:Staining and Labeling