A new protein: macromolecular insoluble could globulin (MICG) derived from mouse thymocytes.
A novel protein synthesized and isolated from mouse thymocytes is described. The protein can be separated from radiolabelled cell lysates by taking advantage of its insolubility in the cold. It is a macromolecule with a molecular weight of 225,000 daltons, migrates as a beta-globulin on electrophoresis and has unique antigenic determinents as shown by the production of a monospecific antiserum.
D000818:Animals; D000937:Antigen-Antibody Reactions; D001609:Beta-Globulins / Q000302:isolation & purification; D011232:Chemical Precipitation; D004591:Electrophoresis, Polyacrylamide Gel; D005916:Globulins / Q000302:isolation & purification*; D007106:Immune Sera / Q000302:isolation & purification; D046911:Macromolecular Substances; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D008970:Molecular Weight; D011817:Rabbits; D012995:Solubility; D013601:T-Lymphocytes / Q000032:analysis*
D001609:Beta-Globulins; D005916:Globulins; D007106:Immune Sera; D046911:Macromolecular Substances