Chromosomal characteristics of six cultured lymphoblastoid cell lines originating from Marek's disease lymphomas.
Cytogenetic observations were made on 6 cell lines (MOB-1, MOB-2, MOB-3, MSB-1, HPRS Line 1, HPRS Line2) originating from Marek's disease lymphomas and 2 clones (1104-B, 1104-X-5) of a cell line established from an avian lymphoid leukosis tumor. The modal chromosome number was within the diploid range in all the lines except HPRS Line 1 and HPRS Line 2, both of which had a mode at about 60. Karyotypes were grossly abnormal in 4 cell lines: trisomy for No. 1 in MOB-2; the heteromorphic No. 1 pair in MSB-1, and marker chromosomes derived from rearrangements involving No. 3 or No. 5 and unidentified elements in HPRS Lines 1 and 2. The MOB-1 line which had been characterized by cells with an apparently normal karyotype was completely taken over by cells with a heteromorphic No. 1 pair morphologically similar to the one found in MSB-1 by the 95th day of continuous growth in vitro. BUdR-acridine orange differential staining technique revealed, however, different banding patterns in these abnormal chromosomes.
D000166:Acridines; D000818:Animals; D001353:Avian Leukosis / Q000235:genetics; D001973:Bromodeoxyuridine; D002460:Cell Line; D002645:Chickens; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000648:ultrastructure; D002999:Clone Cells; D007621:Karyotyping; D008380:Marek Disease / Q000235:genetics*; D008938:Mitosis; D013194:Staining and Labeling
D000166:Acridines; D001973:Bromodeoxyuridine