A cellulose acetate immunofixation technique.
A method of immunofixation using CAM as the only substrate is described. It consists of separating the proteins by conventional electrophoresis followed by immersion for a few minutes only in appropriate specific antiserum suitably diluted with barbitone buffer containing polyethylene glycol (PEG). This is followed by washing and staining by Ponceau S or Nigrosin. The advantages of the method used are speed, sensitivity and economy, no expensive equipment or reagents being required. The method has proved helpful in the identification and classification of minor paraprotein bands and in the analysis of multiclonal paraproteinaemias. The technique can be applied as well to other proteins of biological fluids, e.g. phenotyping.
D000085:Acetates; D002482:Cellulose; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G; D007158:Immunologic Techniques / Q000379:methods; D008566:Membranes; D010266:Paraproteins; D000509:alpha-Fetoproteins
D000085:Acetates; D007106:Immune Sera; D007074:Immunoglobulin G; D010266:Paraproteins; D000509:alpha-Fetoproteins; D002482:Cellulose