Similar polypeptide composition of fast-transported proteins in rat motor and sensory axons.
SDS-polyacrylamide gel electrophoresis was used to characterize labeled proteins transported in rat motor and sensory axons after application of 3H-leucine to the neuron cell bodies. Two types of experiments were performed: first, transported protein accumulating proximal to a ligature placed on the sciatic nerve was analyzed; second, the segment of sciatic nerve nearest to the "wavecrest" of transported protein travelling down the nerve was analyzed. In both cases, no significant differences in peak position or amplitude were found in gels containing labeled proteins from motor or sensory axons. This may mean that the majority of fast-transported protein is involved in an axonal function common to the two types of neuron.
D000818:Animals; D001370:Axonal Transport; D004591:Electrophoresis, Polyacrylamide Gel; D008297:Male; D009046:Motor Neurons / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000378:metabolism*; D009475:Neurons, Afferent / Q000378:metabolism; D051381:Rats; D013127:Spinal Nerves / Q000032:analysis / Q000378:metabolism*
D009419:Nerve Tissue Proteins