Biochemical investigations of separated epithelium and stroma from benign hyperplastic prostatic tissue.
Using arginase and hydroxyproline as biochemical markers, the yields and homogeneity of separated epithelial and stromal tissues from surgically removed benign hyperplastic prostate glands have been assessed. On the basis of these markers, about 30% of epithelial and 95% of stromal tissues were recovered. Dehydroepiandrosterone sulphate sulphatase activity was found predominantly in the epithelium, whereas testosterone 5alpha-reductase activity was predominantly in the stroma.
D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase / Q000378:metabolism; D000368:Aged; D001119:Arginase / Q000378:metabolism; D002469:Cell Separation; D003687:Dehydroepiandrosterone; D004847:Epithelial Cells; D004848:Epithelium / Q000201:enzymology; D006801:Humans; D006909:Hydroxyproline / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D011470:Prostatic Hyperplasia / Q000201:enzymology*; D013429:Sulfatases / Q000378:metabolism
D003687:Dehydroepiandrosterone; D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase; D013429:Sulfatases; D001119:Arginase; D006909:Hydroxyproline