title: Tissue typing of cells in culture. III. HLA antigens of established human cell lines. Attempts at typing by the mixed hemadsorption technique.
abstract: Thirty established cell lines from solid human tumors were typed by the mixed hemadsorption technique with respect to the HLA-A and HLA-B and partly also the HLA-C series. All cultures exhibited unique antigenic patterns. The discriminatory potential was further increased very much taking into consideration one as yet undefined antigen series tentatively named Ek-1 to Ek-11. Sixteen cell lines not designated as HeLa cells but on various non-immunological indications suspected to be HeLa cell contaminants, all gave, with our present test, the same antigenic composition as HeLa cells or clones thereof. The mixed hemadsorption reaction is a highly sensitive and convenient method for immunological typing of cells in monolayer cultures. Some important implications of this are discussed.
mesh_terms: D000951:Antigens, Neoplasm; D002460:Cell Line; D000939:Epitopes; D006680:HLA Antigens; D006382:Hemadsorption; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D009369:Neoplasms / Q000276:immunology
chemical_list: D000951:Antigens, Neoplasm; D000939:Epitopes; D006680:HLA Antigens