In vitro synthesis of infectious DNA of murine leukaemia virus.
DNA synthesised in vitro by purified virions of murine leukaemia virus is infectious. Neither RNA nor protein is required for infectivity. Transfection with reverse trancriptase product shows a single-hit dose response and results in the production of complete, infectious virus.
D002460:Cell Line; D002474:Cell-Free System; D004277:DNA, Single-Stranded / Q000378:metabolism; D004279:DNA, Viral / Q000096:biosynthesis*; D003609:Dactinomycin / Q000494:pharmacology; D008970:Molecular Weight; D008979:Moloney murine leukemia virus / Q000235:genetics*; D012367:RNA, Viral / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D013329:Structure-Activity Relationship; D014162:Transfection; D014779:Virus Replication
D004277:DNA, Single-Stranded; D004279:DNA, Viral; D012367:RNA, Viral; D003609:Dactinomycin; D012194:RNA-Directed DNA Polymerase