A spontaneously occurring complex of beta2-microglobulin and a fragment of gamma-chain of IgG: isolation from the urine of a patient with plasma cell leukemia and characterization.
A complex of a fragment of gamma-chain of IgG and beta2-microglobulin (beta2M) was isolated from the urine of a patient with plasma cell leukemia. The approximate m.w. of the gamma-fragment was 19,000 and this gamma-fragment was found to be associated with beta2M noncovalently. The complex could be dissociated in 7.5% (v/v) n-propanol which suggests an important role of hydrophobic bonds in the association of the gamma-fragment and beta2M. The beta2M did not bind monoclonal IgG from this patient. Several anti-beta2M antisera tested which had been prepared with free beta2M did not react with the beta2M associated with the gamma-fragment, but reacted with the free beta2M obtained by the dissociation of the complex.
D001609:Beta-Globulins / Q000652:urine*; D006801:Humans; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains / Q000652:urine*; D007142:Immunoglobulin gamma-Chains / Q000032:analysis / Q000302:isolation & purification / Q000652:urine*; D007952:Leukemia, Plasma Cell / Q000652:urine*; D013997:Time Factors; D001613:beta 2-Microglobulin / Q000032:analysis / Q000302:isolation & purification / Q000652:urine*
D001609:Beta-Globulins; D007074:Immunoglobulin G; D007143:Immunoglobulin Heavy Chains; D007142:Immunoglobulin gamma-Chains; D001613:beta 2-Microglobulin