Immunochemical purification and characterization of ovine alpha-fetoprotein.
Ovine alpha-fetoprotein was successfully isolated from fetal sheep serum by using rabbit anti-ovine alpha-fetoprotein linked to an agarose immunoadsorbent column. Antibody used in this affinity chromatography column was produced by immunizing a rabbit with highly purified alpha-fetoprotein-antibody complex to yield a monospecific antiserum to ovine alpha-fetoprotein. Following affinity chromatography, alpha-fetoprotein was further purified by preparative polyacrylamide disc gel electrophoresis ultimately yielding a 105-fold purification. The purified alpha-fetoprotein was homogeneous on analytical polyacrylamide disc gel electrophoresis. Ovine alpha-fetoprotein was found to be immunochemically related to human alpha-fetoprotein and to exhibit a molecular weight and amino acid composition similar to other mammalian alpha-fetoproteins.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002645:Chickens / Q000276:immunology; D002846:Chromatography, Affinity; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D008297:Male; D008970:Molecular Weight; D011817:Rabbits / Q000276:immunology; D012756:Sheep; D013045:Species Specificity; D000509:alpha-Fetoproteins / Q000302:isolation & purification; D005719:gamma-Globulins
D000596:Amino Acids; D000509:alpha-Fetoproteins; D005719:gamma-Globulins