Immunofluorescent anti-tubulin staining of spindles during meiotic maturation of mouse oocytes in vitro.
Immunofluorescent anti-tubulin staining has been used to follow nuclear progression from dictyate to metaphase II during meiotic maturation of mouse oocytes in vitro. Antibody directed against tubulin isolated from sea-urchin eggs decorates the metaphase I and metaphase II spindles, as well as the cytoplasmic bridge, midbody, and polar body of the maturing mouse oocytes. Changes in the tubulin-specific staining pattern during meiotic maturation in vitro take place in a highly reproducible manner. Oocytes exposed continuously to cytochalasin B arrest at metaphase I and display a spindle which by immunofluorescent staining is virtually indistinguishable from the spindle of untreated oocytes.
D000818:Animals; D002478:Cells, Cultured; D003571:Cytochalasin B / Q000494:pharmacology; D005260:Female; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000276:immunology; D008540:Meiosis / Q000187:drug effects; D008677:Metaphase; D051379:Mice; D009865:Oocytes / Q000166:cytology* / Q000187:drug effects; D010063:Ovum / Q000166:cytology*; D013194:Staining and Labeling; D014404:Tubulin / Q000276:immunology
D006023:Glycoproteins; D014404:Tubulin; D003571:Cytochalasin B