Leukocyte migration inhibition induced by the combination of drug and a liver constituent in patients with drug-induced hepatitis.
The leukocyte migration inhibition test in agarose medium was performed in 23 cases of clinically diagnosed drug-induced hepatitis. When the test antigen was the combination of soluble phase of a liver homogenate fractionated by Sephadex G-100 which should have contained liver specific antigen and the offending drug the leukocyte migration was inhibited in 86% of cases. Whereas none of 12 cases of drug allergy without hepatic injury showed a positive result with the same combination of antigens. Other organ homogenate-muscle and kidney-never gave positive results when mixed with the offending drugs in cases of drug-induced hepatitis. It was concluded that in hypersensitivity type drug-induced hepatitis cell-mediated immunity might be established to the complex of liver specific antigen and the drug.
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D000941:Antigens / Q000032:analysis; D002464:Cell Migration Inhibition; D056486:Chemical and Drug Induced Liver Injury / Q000175:diagnosis / Q000276:immunology*; D004342:Drug Hypersensitivity / Q000276:immunology; D000939:Epitopes; D005260:Female; D006801:Humans; D007668:Kidney / Q000276:immunology; D007962:Leukocytes / Q000276:immunology*; D008099:Liver / Q000166:cytology / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D051381:Rats
D000941:Antigens; D000939:Epitopes