Synthesis of Sindbis virus complementary DNA by avian myeloblastosis virus RNA-directed DNA polymerase.
Sindbis virus 42 S RNA was efficiently transcribed into complementary DNA (CDNA) by avian myeloblastosis virus alphabeta DNA polymerase using oligo- (dT) or single-stranded calf thymus DNA as primers. Both of the Sindbis virus cDNA products were able to protect 60% of 125I-labeled Sindbis virus RNA, at near equal weight ratios, from RNAase A and T1 digestion. Using hybridization kinetics, the Crt 1/2 value for hybridization of the calf thymus-primed cDNA product with excess Sindbis RNA was determined to be 1.8 9 10-2 mol . s . 1-1. Thes data demonstrate that the Sindbis virus cDNA products are relatively uniform representations of Sindbis virus RNA sequences.
D009189:Avian Myeloblastosis Virus / Q000201:enzymology; D001483:Base Sequence; D004247:DNA / Q000378:metabolism; D004279:DNA, Viral / Q000096:biosynthesis*; D008722:Methods; D008970:Molecular Weight; D009695:Nucleic Acid Renaturation; D009838:Oligodeoxyribonucleotides / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*; D012845:Sindbis Virus / Q000235:genetics
D004279:DNA, Viral; D009838:Oligodeoxyribonucleotides; D004247:DNA; D012194:RNA-Directed DNA Polymerase