The allergenic structure of allergen M from cod. I. Tryptic peptides of fragment TM 1.
In attempts to locate the allergenic active sites of fragment TM 1 of allergen M from cod, the allergenicity of the tryptic hydrolysis peptides was examined both in vivo and in vitro. A duodecapeptide (residues No. 33-44) was tentatively suggested to incorporate at least one allergenic determinant. All the tryptic peptides were less active than allergen M as shown by the in vivo titration experiments. The activity of the peptides was not due to contamination of intact allergens.
D000485:Allergens; D000595:Amino Acid Sequence; D000818:Animals; D000939:Epitopes; D005399:Fishes; D010446:Peptide Fragments / Q000276:immunology; D010455:Peptides / Q000302:isolation & purification; D014357:Trypsin
D000485:Allergens; D000939:Epitopes; D010446:Peptide Fragments; D010455:Peptides; D014357:Trypsin