The effect of bleomycin on mitochondrial DNA.
Covalently closed circular mitochondrial DNA (mt-DNA) from mouse fibroblasts (L-cells) forms a separate band from nicked circular or linear DNA molecules in ethidium bromide-cesium chloride byoyant density gradient analysis. The conversion of covalently closed circular mt-DNA to nicked circular or linear DNA may serve as a sensitive method of detecting DNA strand breaks induced by bleomycin (BLM). At a very high concentration BLM induces the conversion of closed circular mt-DNA to nicked circular or linear DNA, however even after 24 h of exposure to BLM at a high concentration a considerable amount of closed circular mt-DNA molecules remains intact.
D000818:Animals; D001761:Bleomycin / Q000494:pharmacology*; D002499:Centrifugation, Density Gradient; D004270:DNA, Circular; D004272:DNA, Mitochondrial; D051379:Mice; D009690:Nucleic Acid Conformation / Q000187:drug effects
D004270:DNA, Circular; D004272:DNA, Mitochondrial; D001761:Bleomycin