Suppressor cell induction in vitro. I. Kinetics of induction of antigen-specific suppressor cells.
The induction of antigen-specific suppressor cells in vitro, using high concentrations (100 mug/ml) of keyhole limpet hemocyanin (KLH) in Marbrook flasks is described. Spleen and cortisone-resistant thymocytes were the richest source of suppressor cell precursors, compared to lymph node cells, peripheral blood lymphocytes or thoracic duct lymphocytes. Suppressor cells induced with KLH only suppressed KLH-reactive helper cells, and not B cells or helper cells of other specificity. The suppressor cells were T cells, as judged by their sensitivity to anti-Thy-1.2, heterologous anti-T, but not anti-B antisera.
D000818:Animals; D000917:Antibody Formation; D000941:Antigens; D002478:Cells, Cultured; D003348:Cortisone / Q000494:pharmacology; D004351:Drug Resistance; D000939:Epitopes; D006433:Hemocyanins / Q000276:immunology; D007165:Immunosuppression Therapy; D007700:Kinetics; D008198:Lymph Nodes / Q000276:immunology; D051379:Mice; D008808:Mice, Inbred CBA; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000941:Antigens; D000939:Epitopes; D006433:Hemocyanins; D003348:Cortisone