Immunodiffusion method for identification of group A streptococci.
The development of a method suitable for identification of group A streptococci by microprecipitation in gels is described. The method is based on preparation of specific sera containing high antibody levels agains the antigenic determinant characteristic of group A streptococcal polysaccharide. In a comparative study with a counterimmunoelectrophoresis method, the proposed test proved to be specific, easily read, and less complicated. Results were obtained in 2 h.
D000907:Antibodies, Bacterial / Q000032:analysis; D000942:Antigens, Bacterial / Q000032:analysis*; D000939:Epitopes; D005779:Immunodiffusion / Q000379:methods; D008050:Lipid A / Q000032:analysis / Q000276:immunology; D013297:Streptococcus pyogenes / Q000145:classification* / Q000276:immunology
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D000939:Epitopes; D008050:Lipid A