Increased synthesis and expression of H-2 antigens on thymocytes as a result of radiation leukemia virus infection: a possible mechanism for H-2 linked control of virus-induced neoplasia.
Previous studies from this laboratory have mapped resistance and/or susceptibility to radiation-induced leukemia virus (RadLV)-induced neoplasia to the H-2D region. H-2 linked effects on virus replication can be detected subsequent to the initial virus infection, and clear-cut differences in numbers of virus infected thymus cells can be detected as early as 5 wk after RadLV inoculation. Rapid increases in cellular synthesis and cell surface expression of H-2 antigens are detectable immediately after virus inoculation. These changes have been studied by immunofluorescence, absorption, cell surface iodination followed by sodium dodecyl-sulfate-polyacrylamide gel electrophoresis, and two dimensional gel electrophoretic analysis of internally labeled lymphocyte proteins. Expression of H-2K molecules is significantly increased in cells of susceptible and resistant animals. However, significant increases in expression of H-2D antigens occurs only on thymus cells from resistant strains (H-2Dd). Transformed cells of resistant and susceptible H-2 haplotypes adapted to tissue culture lack detectable H-2 antigens as determined by serological absorption studies. It is argued that altered expression of H-2 antigens plays a very significant role in the mechanism of host defense to virus infection.
D000818:Animals; D002455:Cell Division; D000939:Epitopes; D008040:Genetic Linkage; D006183:H-2 Antigens / Q000235:genetics; D009052:Leukemia Virus, Murine; D007942:Leukemia, Experimental / Q000235:genetics / Q000276:immunology*; D051379:Mice; D013945:Thymoma / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology*; D013953:Thymus Neoplasms / Q000276:immunology; D014779:Virus Replication
D000939:Epitopes; D006183:H-2 Antigens