title: Separation of leukemic cells into proliferative and quiescent subpopulations by centrifugal elutriation.
abstract: Centrifugal elutriation was used to separate human acute leukemia cells into proliferative and quiescent subpopulations. Ten bone marrow specimens and 5 peripheral blood specimens were subjected to centrifugal elutriations. From each patient, leukemic cell subpopulations were obtained for which the [3H]thymidine labeling index differed by 10- to 30-fold. In 6 of the marrow specimens and in 2 of the peripheral blood specimens, cell subpopulations were obtained for which the labeling index exceeded 20%. In 5 marrow specimens, subpopulations were obtained for which the labeling index exceeded 40%. Preliminary studies of the uptake of 1-beta-D-arabinofuranosylcytosine and 5-azacytidine failed to show any correlation between drug uptake and the proliferative characteristics of the leukemic subpopulations.
mesh_terms: D001374:Azacitidine / Q000378:metabolism; D001853:Bone Marrow / Q000378:metabolism / Q000473:pathology; D002455:Cell Division; D002469:Cell Separation / Q000379:methods*; D002498:Centrifugation; D003561:Cytarabine / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007945:Leukemia, Lymphoid / Q000378:metabolism / Q000473:pathology*; D007951:Leukemia, Myeloid / Q000378:metabolism; D015470:Leukemia, Myeloid, Acute / Q000473:pathology*; D013936:Thymidine / Q000378:metabolism
chemical_list: D003561:Cytarabine; D001374:Azacitidine; D013936:Thymidine