Propagation of antigen-specific T cell helper function in vitro.
Antigen-induced proliferation of primed lymph node cells was abrogated by treatment with anti-Ly 1 serum and complement (C) but not with anti-Ly 2 serum and C. Lymph node cells from animals primed to ovalbumin were activated with antigen in vitro, followed by propagation in an antigen-free supernatant fluid obtained from lectin-induced normal spleen cells. T cells processed in this manner displayed a stepwise enrichment of helper activity for antibody production as measured in a secondary hapten-carrier response. The sequential increase in antigen-specific help was paralleled by a rise in the antigen-induced proliferative response, a phenomenon whose expression was dependent on the presence of syngeneic or semi-syngeneic irradiated filler cells.
D000818:Animals; D000961:Antilymphocyte Serum / Q000494:pharmacology; D003165:Complement System Proteins / Q000276:immunology; D003208:Concanavalin A / Q000494:pharmacology; D003470:Culture Media; D004136:Dinitrobenzenes / Q000276:immunology; D000939:Epitopes; D006433:Hemocyanins / Q000276:immunology; D006462:Hemolytic Plaque Technique; D006801:Humans; D008198:Lymph Nodes / Q000276:immunology; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008285:Major Histocompatibility Complex; D051379:Mice; D008805:Mice, Inbred A; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D010047:Ovalbumin / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D005719:gamma-Globulins / Q000276:immunology
D000961:Antilymphocyte Serum; D003470:Culture Media; D004136:Dinitrobenzenes; D000939:Epitopes; D005719:gamma-Globulins; D003208:Concanavalin A; D010047:Ovalbumin; D003165:Complement System Proteins; D006433:Hemocyanins