Purification and characterisation of two proteins from Ascaris suum extract, antigenically different but bearing common allergenic epitopes.
Two proteins, FI and FVII, have been purified from a whole worm extract of adult Ascaris suum, using ammonium sulfate precipitation, Sephadex gel filtration and DEAE cellulose chromatography. Both of them provoke reagin formation in mice as tested in presensitized animals with an original immediate hypersensitivity reaction test. They are two chemically and physically different protein molecules showing no cross reaction with a rabbit serum anti-crude extract but bearing common allergenic epitopes as proved when tested in mice.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000941:Antigens / Q000032:analysis / Q000302:isolation & purification*; D001200:Ascaris / Q000276:immunology*; D002241:Carbohydrates / Q000032:analysis; D011232:Chemical Precipitation; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D003429:Cross Reactions; D000939:Epitopes; D006969:Hypersensitivity, Immediate; D051379:Mice; D008970:Molecular Weight; D011817:Rabbits
D000596:Amino Acids; D000941:Antigens; D002241:Carbohydrates; D000939:Epitopes