Enhancement by cytotoxic agents of artificial pulmonary metastasis.
The formation of lung colonies by i.v. injected Lewis lung-tumour cells in syngeneic recipients was greatly enhanced by prior treatment of the mice with cyclophosphamide. The lung-cloning efficiency was linearly related to cyclophosphamide dose and the optimum time of treatment was 2-4 days before the injection of tumour cells. The resulting lung colonies had a similar size distribution to colonies in untreated recipients. Bleomycin, local thoraric irradiation and whole-body irradiation were much less effective in enhancing the lung-cloning efficiency. Cyclophosphamide also enhanced the take probability of i.m. implanted tumour cells.
D000818:Animals; D001761:Bleomycin / Q000494:pharmacology; D002999:Clone Cells / Q000187:drug effects; D003520:Cyclophosphamide / Q000494:pharmacology*; D008175:Lung Neoplasms; D051379:Mice; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental; D011830:Radiation Effects; D013268:Stimulation, Chemical; D014184:Transplantation, Homologous
D001761:Bleomycin; D003520:Cyclophosphamide