Influence of mounting media on the fading of basic aniline dyes in epoxy embedded tissues.
A simple cytophotometric technique is used to quantitate stain fading of basic aniline dye-stained epoxy-embedded tissues mounted in six different commonly used mountants. Significant fading was detected with all six mountants, although rates varied. The lowest rate of fading was observed with immersion oil and the highest rate of fading with Canada balsam. No significant differences in fading rates of four synthetic mounting preparations were observed.
D000818:Animals; D003106:Colon / Q000033:anatomy & histology; D003720:Densitometry; D004853:Epoxy Resins; D005069:Evaluation Studies as Topic; D006652:Histological Techniques; D006801:Humans; D007413:Intestinal Mucosa / Q000033:anatomy & histology; D008722:Methods; D051381:Rats; D012394:Rosaniline Dyes; D012867:Skin / Q000033:anatomy & histology; D013194:Staining and Labeling
D004853:Epoxy Resins; D012394:Rosaniline Dyes