Histone-produced magnesium extrusion from mitochondria and magnesium binding to histone.
Histone (60 microgram/mg mit. protein) extrudes Mg2+ from mitochondria by 30% with the utilization of endogenous substrates; in the presence of rotenone extrusion drops to about 18%. Dinitrophenol and ADP prevent this effect of histone. Mg2+ extrusion produced by histone depends on histone concentration being at a maximum (100% extrusion) at 107 microgram histone/mit. protein. It was found also that histone alone binds Mg2+ (1.6 nmol Mg2+/microgram histone).
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D004140:Dinitrophenols / Q000494:pharmacology; D006657:Histones / Q000378:metabolism / Q000494:pharmacology*; D066298:In Vitro Techniques; D008274:Magnesium / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D011485:Protein Binding; D051381:Rats
D004140:Dinitrophenols; D006657:Histones; D000244:Adenosine Diphosphate; D008274:Magnesium