The cytolytic effect of PUVA treatment on PHA-stimulated human peripheral lymphocytes.
The morphology of PUVA-treated and PHA-stimulated human peripheral lymphocytes in vitro was studied by means of the light and electron microscopes. Most cells showed signs of cytolysis, such as pyknotic nuclei and a swollen and decomposed cytoplasm; a few cells, however, appeared as normal lymphoblasts. In the latter, repair-enzyme mechanisms may successfully have rectified alterations in the DNS prior to the inception of S-phase. These findings are discussed with reference to the in vivo effect of PUVA treatment.
D002467:Cell Nucleus / Q000648:ultrastructure; D003374:Coumarins / Q000494:pharmacology*; D003593:Cytoplasm / Q000648:ultrastructure; D005363:Ficusin / Q000494:pharmacology*; D006801:Humans; D037102:Lectins / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000187:drug effects* / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D010778:Photochemotherapy; D014466:Ultraviolet Rays
D003374:Coumarins; D037102:Lectins; D005363:Ficusin