Simple, differential staining technique for enumerating rickettsiae in yolk sac, tissue culture extracts, or purified suspensions.
A differential staining method employing acridine orange-stained rickettsiae and safranin-stained standardized suspensions of Shigella dysenteriae is described for enumerating rickettsiae harvested during various stages of the growth cycle.
D000165:Acridine Orange; D000818:Animals; D002642:Chick Embryo; D003381:Coxiella / Q000302:isolation & purification; D046508:Culture Techniques; D012281:Rickettsia / Q000302:isolation & purification*; D012283:Rickettsia prowazekii / Q000302:isolation & purification; D012762:Shigella dysenteriae; D013194:Staining and Labeling / Q000379:methods* / Q000592:standards; D013535:Suspensions; D015017:Yolk Sac / Q000382:microbiology
D013535:Suspensions; D000165:Acridine Orange