Topographical studies on intestinal microvillous leucine beta-naphthylamidase on the outer membrane surface.
The location of leucine beta-naphthylamidase on the outer surface of the microvillous membrane of rabbit small intestine was examined by analyzing the interaction of antibodies against leucine beta-naphthylamidase or another microvillous enzyme, sucrase-isomaltase complex, with microvillous vesicles having different relative amounts of these enzymes, in respect to vesicle agglutination, inhibition of enzyme activity, and electron-microscopic morphology. The results obtained indicate that leucine beta-naphthylamidase, or at least its antigenic sites, protrude about 10 nm from the outer surface of the microvillous membrane.
D000818:Animals; D000939:Epitopes; D007158:Immunologic Techniques; D007413:Intestinal Mucosa / Q000201:enzymology / Q000648:ultrastructure*; D007931:Leucyl Aminopeptidase / Q000276:immunology / Q000378:metabolism*; D008854:Microscopy, Electron; D008871:Microvilli / Q000201:enzymology / Q000648:ultrastructure; D011817:Rabbits; D013394:Sucrase-Isomaltase Complex / Q000378:metabolism
D000939:Epitopes; D013394:Sucrase-Isomaltase Complex; D007931:Leucyl Aminopeptidase