Precipitation of radiolabeled antigen-antibody complexes with protein A-containing Staphylococcus aureus.
The feasibility of using protein A-containing Staphylococcus aureus to measure antibodies in sera from several mammalian species was studied. A variety of unrelated radiolabeled antigens were tested, including components of bovine serum, DNA, and bacterial and tumor-associated extracts. The use of S. aureus was found to be a reliable way to detect and measure the primary interactions between many of the antigens and antibodies tested. Results were equivalent under many circumstances to those obtained with the ammonium sulfate and heterologous anti-immunoglobulin methoods. However, some of the limitations noted were that certain antigens bound directly to S. aureus and that all classes of human immunoglobulins tested, in particular IgG3 and IgA1, were not precipitated by S. aureus. If these limitations are taken into consideration, the use of S. aureus can be of value in studying immunochemical reactions with other antigens.
D000645:Ammonium Sulfate / Q000494:pharmacology; D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D000936:Antigen-Antibody Complex; D000941:Antigens; D001500:BCG Vaccine; D001426:Bacterial Proteins / Q000494:pharmacology*; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D002417:Cattle; D004247:DNA / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D006168:Guinea Pigs; D006801:Humans; D007070:Immunoglobulin A / Q000378:metabolism; D007074:Immunoglobulin G / Q000378:metabolism; D007075:Immunoglobulin M / Q000378:metabolism; D009163:Mycobacterium bovis / Q000276:immunology; D009194:Myeloma Proteins / Q000276:immunology; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000276:immunology; D013211:Staphylococcus aureus / Q000276:immunology*; D005719:gamma-Globulins / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D000936:Antigen-Antibody Complex; D000941:Antigens; D001500:BCG Vaccine; D001426:Bacterial Proteins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D009194:Myeloma Proteins; D005719:gamma-Globulins; D012710:Serum Albumin, Bovine; D004247:DNA; D000645:Ammonium Sulfate