Determination of growth of aquatic bacteria by measurements of incorporation of tritiated thymidine.
Evaluation was made of a novel technique, combining semi-continuous culture on membrane filters and assessment of the incorporation of titrated thymidine. The optimal temperature of incubation is 20--25 degrees, the period of incubation--3 hours; the initial activity of tritiated thymidine--0.5 muC/ml. There is a linear relation between the initial number of bacteria on a filter and the level of 3H-thymidine incorporation. The incorporation is dependent on nutrient content in the examined water.
D000085:Acetates / Q000378:metabolism; D004261:DNA Replication; D004269:DNA, Bacterial / Q000096:biosynthesis; D005618:Fresh Water; D008851:Micropore Filters; D011549:Pseudomonas / Q000254:growth & development* / Q000378:metabolism; D013696:Temperature; D013936:Thymidine / Q000378:metabolism*; D014316:Tritium; D014871:Water Microbiology
D000085:Acetates; D004269:DNA, Bacterial; D014316:Tritium; D013936:Thymidine