Simultaneous determination of phosphatidylglycerol and the lecithin/sphingomyelin ratio in amniotic fluid.
We describe a single-dimension thin-layer chromatographic method by which one can simultaneously determine the lecithin/sphingomyelin ratio and the proportion of phosphatidylglycerol in amniotic fluid. The phospholipids are conveniently detected by an improved molybdenum-blue spray reagent, which immediately produces blue spots on a white background. The stained phospholipids are stable for at least 90 min after spraying, and densitometry results in symmetrical peaks that are easily quantitated.
D000653:Amniotic Fluid / Q000032:analysis*; D002855:Chromatography, Thin Layer / Q000379:methods; D003720:Densitometry; D005260:Female; D006801:Humans; D007202:Indicators and Reagents; D008982:Molybdenum; D010713:Phosphatidylcholines / Q000032:analysis*; D010715:Phosphatidylglycerols / Q000032:analysis*; D011247:Pregnancy; D013109:Sphingomyelins / Q000032:analysis*; D013194:Staining and Labeling
D007202:Indicators and Reagents; D010713:Phosphatidylcholines; D010715:Phosphatidylglycerols; D013109:Sphingomyelins; D008982:Molybdenum