Possible detection of HLA-DR alloantigenic specificities in man with unabsorbed rabbit antisera.
Thirty-six rabbits were immunized with precipitin lines formed between detergent solubilized membrane fractions from HLA-D-homozygous cells and a rabbit antiserum to human B cells (anti-p 23/30). Thirty-one of the rabbits produced B-cell specific cytotoxic antibodies and twelve of the antibodies were also precipitating in gel diffusion. In cytotoxicity tests six of the antisera showed clear preferential reactivity with the immunizing HLA-D (DR) antigen. Thus immunization of rabbits might prove valuable for the production of HLA-DR typing reagents.
D000818:Animals; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D011232:Chemical Precipitation; D003601:Cytotoxicity Tests, Immunologic; D003902:Detergents; D000939:Epitopes; D005260:Female; D006680:HLA Antigens / Q000032:analysis; D006801:Humans; D007106:Immune Sera; D007114:Immunization; D007519:Isoantigens; D011817:Rabbits / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
D003902:Detergents; D000939:Epitopes; D006680:HLA Antigens; D007106:Immune Sera; D007519:Isoantigens