title: Effect of various reagents on the susceptibility of target cells to the cytotoxicity of guinea pig lymphotoxin (GLT).
abstract: The cytotoxic activity of guinea pig lymphotoxin (GLT) was increased when the target cells were treated with the following metabolic inhibitors: puromycin, actinomycin D, NaN3, NaF, and 2,4-dinitrophenol, whereas calf serum, N-acetyl-D-galactosamine, colchicine, and vinblastin inhibited GLT-cytotoxicity. Puromycin, an inhibitor of protein synthesis, exhibited the most potent enhancing effect on GLT-cytotoxicity. It seems likely that there are certain cellular metabolic processes depending on cellular protein synthesis which antagonize GLT-cytotoxicity. Taking advantage of this effect of puromycin, a time-saving assay method for GLT-cytotoxicity was developed.
mesh_terms: D000818:Animals; D001427:Bacterial Toxins / Q000494:pharmacology; D003078:Colchicine / Q000494:pharmacology; D003601:Cytotoxicity Tests, Immunologic; D003609:Dactinomycin / Q000494:pharmacology; D004136:Dinitrobenzenes / Q000494:pharmacology; D004306:Dose-Response Relationship, Immunologic; D006168:Guinea Pigs; D008214:Lymphocytes / Q000187:drug effects; D008233:Lymphotoxin-alpha / Q000032:analysis*; D010947:Plants, Toxic; D011691:Puromycin / Q000494:pharmacology; D012278:Ricinus; D012969:Sodium Fluoride / Q000494:pharmacology; D014747:Vinblastine / Q000494:pharmacology
chemical_list: D001427:Bacterial Toxins; D004136:Dinitrobenzenes; D008233:Lymphotoxin-alpha; D003609:Dactinomycin; D011691:Puromycin; D014747:Vinblastine; D012969:Sodium Fluoride; D003078:Colchicine