A silver impregnation method for nervous tissue suitable for routine use with mounted sections.
A simple, reliable silver impregnation method for nervous tissue is described for tissues fixed in various fixatives including formalin, Bouin, and Susa. Sections are impregnated in a solution containing 1 g Protargol, 2 ml of a 1% Cu(NO3)2 solution, 2 ml of a 1% AgNO3 solution, and 2-4 drops 30% H2O2 in 100 ml distilled water. Sections are impregnated 2-5 days at 37 C and thereafter reduced in a hydroquinone-formalin solution. This is followed by gold toning and subsequent reduction, dehydration and mounting. This method has been found to be very reliable and selective.
D000818:Animals; D001369:Axons / Q000166:cytology; D002415:Cats; D002645:Chickens; D003094:Collagen; D005404:Fixatives; D006801:Humans; D009417:Nerve Tissue / Q000166:cytology*; D009454:Neurofibrils / Q000166:cytology; D011817:Rabbits; D051381:Rats; D012834:Silver; D013194:Staining and Labeling
D005404:Fixatives; D012834:Silver; D003094:Collagen