Preparation of homogeneous human prostatic acid phosphatase using concanavalin A-sepharose 4-B.
A simple, rapid and efficient procedure is presented for the purification of human prostatic acid phosphatase (orthophosphoric-monoester phosphohydrolase (acid optimum), EC 3.1.3.2) to homogeneity. The method employs two steps suitable for use with large quantities of material, followed by chromatography on concanavalin A-Sepharose as its sole column step. The procedure also permits the recovery of purified enzyme in higher yields than earlier methods.
D000135:Acid Phosphatase / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D003208:Concanavalin A; D006801:Humans; D008297:Male; D011467:Prostate / Q000201:enzymology*; D011470:Prostatic Hyperplasia / Q000201:enzymology
D003208:Concanavalin A; D000135:Acid Phosphatase