title: Binding properties of goat IgM anti-dinitrophenyl antibodies.
abstract: Goats immunized over 2 months with low doses of 1 mg/kg of dinitrophenylated Salmonella typhimurium responded with low levels of anti-DNP antibodies restricted to the IgM class. The purified antibodies show low association constants (Ka between 10(4) of 10(5) l/M), a high degree of homogeneity (heterogeneity indices alpha between 0.7 and 0.9) and ten combining sites when tested against dinitrophenyl-lysine as ligand by equilibrium dialysis. These binding properties remained unchanged during the whole immune response. When after 9 months the animals received the same immunogen and DNP-BGG, the anti-DNP antibody response included antibodies in the IgG class.
mesh_terms: D000818:Animals; D000915:Antibody Affinity; D001666:Binding Sites, Antibody; D004136:Dinitrobenzenes / Q000276:immunology; D006041:Goats; D007114:Immunization; D007074:Immunoglobulin G / Q000032:analysis / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000032:analysis / Q000096:biosynthesis*; D007525:Isoelectric Focusing; D012486:Salmonella typhimurium / Q000276:immunology; D005719:gamma-Globulins / Q000276:immunology
chemical_list: D004136:Dinitrobenzenes; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D005719:gamma-Globulins