title: Characterization of human papovavirus RFV: comparison with SV40 and BKV.
abstract: Human papovavirus, RFV, isolated from urine of a renal transplant patient was compared with two strains of SV40 and with the prototype human papovavirus, BKV. Neutralization tests showed that RFV and BKV are indistinguishable, while large-plaque (LP) and small-plaque(SP) isolates of SV40 gave a low but significant level of cross-reaction with rabbit or human antisera against RFV. DNA reassociation saturation tests using 125I-labelled RFV DNA show that BKV has 88% homology, and SP-SV40 has 29% homology to RFV. We conclude that RFV and BKV are nearly, if not totally, identical and are not SV40 variants.
mesh_terms: D000956:Antigens, Viral / Q000032:analysis; D001739:BK Virus / Q000032:analysis / Q000276:immunology; D004279:DNA, Viral / Q000032:analysis; D000939:Epitopes; D006801:Humans; D016030:Kidney Transplantation; D009690:Nucleic Acid Conformation; D027383:Papillomaviridae / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification; D027382:Polyomaviridae; D011120:Polyomavirus / Q000276:immunology; D013539:Simian virus 40 / Q000032:analysis / Q000276:immunology; D014556:Urine / Q000382:microbiology
chemical_list: D000956:Antigens, Viral; D004279:DNA, Viral; D000939:Epitopes