Pluripotent teratocarcinoma-thymus somatic cell hybrids.
We have produced a series of somatic cell hybrids by fusing pluripotent PCC4aza1 embryonal carcinoma ("teretocarcinoma") cells with thymocytes from young adult mice. When these hybrids form tumors in nu/nu or syngeneic mice, all the tumors contain a range of differentiated tissues, as well as embryonal carcinoma-like tissues. Some of the tumors produce alpha-fetoprotein. These results show that pluripotency in embryonal carcinoma cells need not to be abolished by the introduction of a complete diploid genome from a differentiated cell.
D000818:Animals; D002459:Cell Fusion; D002460:Cell Line; D005956:Glucose-6-Phosphate Isomerase / Q000378:metabolism; D006822:Hybrid Cells / Q000201:enzymology; D007621:Karyotyping; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000209:etiology / Q000473:pathology; D013601:T-Lymphocytes; D013724:Teratoma / Q000378:metabolism / Q000473:pathology; D000509:alpha-Fetoproteins / Q000096:biosynthesis
D000509:alpha-Fetoproteins; D005956:Glucose-6-Phosphate Isomerase