Comparison of cell culture with epimastigote antigens of Trypanosoma cruzi.
Various epimastigote antigens of Trypanosoma cruzi were compared with an amastigote-trypomastigote antigen from infected cell cultures by complement fixation (CF) and gel diffusion (Ouchterlony). CF results with human Chagasic sera showed that the amastigote-trypomastigote antigen was usually more sensitive than epimastigote antigens tested. In addition, cross-reactions with normal and other sera were no greater and perhaps less frequent than with crude epimastigote antigens. Specificity of the amastigote-trypomastigote antigen, however, was less than with a protein extract of epimastigotes. Gel diffusion results with human Chagasic and hyper-immunized rabbit sera indicated differences between epimastigote and amastigote-trypomastigote antigens whereas differences by CF with the same sera were equivocal.
D000818:Animals; D000941:Antigens; D002478:Cells, Cultured; D014355:Chagas Disease / Q000276:immunology; D003168:Complement Fixation Tests; D003429:Cross Reactions; D003470:Culture Media; D000939:Epitopes; D006801:Humans; D007896:Leishmaniasis / Q000276:immunology; D011817:Rabbits; D013587:Syphilis / Q000276:immunology; D014349:Trypanosoma cruzi / Q000166:cytology / Q000276:immunology*
D000941:Antigens; D003470:Culture Media; D000939:Epitopes