A modified technique for combination of cytochemistry and radioautography of haemic cells.
Cultured mouse bone marrow and human blood cells were labelled with 3H-thymidine, smeared, and stained, using peroxydase reagents or the May-Grünwald Giemsa procedure. The slides were dipped in a approximately 12% solution of polyvinylchloride, bleached by a photographic fixer and processed for liquid emulsion radioautography. This method yielded technically very satisfactory radioautographs of pre-stained smears.
D000818:Animals; D001345:Autoradiography; D001399:Azure Stains; D001773:Blood Cells / Q000166:cytology*; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D004000:Dichloroethylenes; D004058:Diffusion; D006651:Histocytochemistry / Q000379:methods*; D006801:Humans; D008297:Male; D008567:Membranes, Artificial; D051379:Mice; D008815:Mice, Inbred Strains; D009000:Monocytes / Q000166:cytology; D010544:Peroxidases; D013194:Staining and Labeling; D014752:Vinyl Chloride
D001399:Azure Stains; D004000:Dichloroethylenes; D008567:Membranes, Artificial; D010544:Peroxidases; D014752:Vinyl Chloride