Detection by antihapten antibodies of liver-bound compounds related to azocarcinogens or their metabolites.
The localization of known azocarcinogens and metabolites such as p-aminoazobenzene and N-methyl-p-aminoazobenzene bound to components of liver cells of rats fed single or multiple doses of 3'-methyl-p-dimethylaminoazobenzene has been determined with the use of antibodies raised against p'-azo-p-aminoazobenzene and p'-azo-N-monomethyl-p-aminoazobenzene in the indirect fluorescent antibody procedure. These 2 antisera reacted with liver cells of rats fed 3'-methyl-p-dimethylaminoazobenzene, p'-amino-p-aminoazobenzene, p'-amino-N-monomethyl-p-aminoazobenzene and N-methyl-p-aminoazobenzene. The results obtained in this study suggest that both major and minor metabolites of azocarcinogens have common antigenic determinants.
D000818:Animals; D000906:Antibodies; D001391:Azo Compounds / Q000276:immunology*; D000939:Epitopes; D006241:Haptens / Q000276:immunology; D008099:Liver / Q000378:metabolism*; D008297:Male; D008749:Methyldimethylaminoazobenzene / Q000031:analogs & derivatives* / Q000378:metabolism*; D051381:Rats; D011919:Rats, Inbred Strains; D010128:p-Aminoazobenzene / Q000031:analogs & derivatives / Q000276:immunology*; D004124:p-Dimethylaminoazobenzene / Q000031:analogs & derivatives*
D000906:Antibodies; D001391:Azo Compounds; D000939:Epitopes; D006241:Haptens; C021142:4'-azo-N-monomethyl-4-aminoazobenzene; D008749:Methyldimethylaminoazobenzene; D010128:p-Aminoazobenzene; D004124:p-Dimethylaminoazobenzene