Isolation and partial characterization of I antigen from I adult erythrocyte membranes.
The i-active substance was isolated from i adult and I int erythrocyte membranes by butanol-water extraction and was purified by SDS-gel filtration and thin layer chromatography of peracetylated samples. The i-active substances from both types of erythrocytes showed also IF activity. In antigen isolated from i adult red cells the IF activity was lower than i activity. On the other hand, the antigen of I int red cells showed higher IF than i activity. The chemical characterization of isolated antigens indicated their glycosphingolipid character.
D001789:Blood Group Antigens; D002241:Carbohydrates / Q000097:blood / Q000276:immunology; D000939:Epitopes; D004910:Erythrocyte Membrane / Q000276:immunology*; D004912:Erythrocytes / Q000276:immunology*; D006028:Glycosphingolipids / Q000097:blood* / Q000276:immunology; D006801:Humans; D007048:I Blood-Group System; D007519:Isoantigens / Q000302:isolation & purification*; D008970:Molecular Weight
D001789:Blood Group Antigens; D002241:Carbohydrates; D000939:Epitopes; D006028:Glycosphingolipids; D007048:I Blood-Group System; D007519:Isoantigens