Characterization of an RNA-directed DNA-polymerase from a cell line derived from a radiation-induced lymphoma in mice.
An RNA-directed DNA polymerase was purified from a cell line derived from a radiation-induced lymphoma in NIH Swiss mice which produced non-infectious type C virus particles. The enzyme was isolated from a high speed particulate fraction which bands at a density of 1.16--1.19 g/ml in a sucrose gradient, and purified by successive chromatography on DEAE-cellulose, phosphocellulose and hydroxyapatite. The purified DNA polymerase has a molecular weight of 68 000, a pH optimum of 7.5, a KCl optimum of 50 mM, and a Mn2+ optimum of 0.25 mM. It prefers (dT)15 . (A)n to (dT)15 . (dA)n as the primer template and transcribes the poly(C) strand of (dG)15 .(C)n and (dG)15 . (OMeC)n. It transcribes heteropolymeric regions of avian myeloblastosis virus 70 S RNA, and is inhibited by antiserum to Rauscher murine leukemia virus DNA polymerase. Comparison of the properties of DNA polymerase purified from radiation-induced lymphoma cells with the DNA polymerase purified from non-defective murine type C RNA tumor viruses shows that the mouse lymphoma enzyme is both biochemically and immunologically related to murine leukemia virus DNA polymerases.
D000818:Animals; D002460:Cell Line; D007118:Immunoassay; D007074:Immunoglobulin G; D007700:Kinetics; D008223:Lymphoma / Q000201:enzymology*; D008345:Manganese / Q000494:pharmacology; D051379:Mice; D008970:Molecular Weight; D009381:Neoplasms, Radiation-Induced / Q000201:enzymology*; D011189:Potassium Chloride / Q000494:pharmacology; D012194:RNA-Directed DNA Polymerase / Q000302:isolation & purification / Q000378:metabolism*; D013698:Templates, Genetic
D007074:Immunoglobulin G; D008345:Manganese; D011189:Potassium Chloride; D012194:RNA-Directed DNA Polymerase