Lipopolysaccharide interferes with the staining of lipoprotein on polyacrylamide gels.
After electrophoresis of total membrane preparations of Escherichia coli B on sodium dodecyl sulfate polyacrylamide gels, and subsequent staining with Coomassie Brilliant blue, a band corresponding to the Braun lipoprotein fails to appear. This is in contrast to similar preparations of E. coli K-12 which do display the lipoprotein upon staining. Experiments described below indicate that failure to observe this protein in E. coli B is due to interference in the staining reaction by the lipopolysaccharide present in the membrane preparations.
D001426:Bacterial Proteins / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004926:Escherichia coli / Q000032:analysis; D008070:Lipopolysaccharides; D008074:Lipoproteins / Q000032:analysis*; D008565:Membrane Proteins / Q000032:analysis; D013194:Staining and Labeling / Q000379:methods*
D001426:Bacterial Proteins; D008070:Lipopolysaccharides; D008074:Lipoproteins; D008565:Membrane Proteins