Induction of type C RNA virus from cultured rabbit lymphosarcoma cells.
Type C RNA virus(es) was readily released from primary lymphosarcoma cell cultures of WH/J rabbits after induction with halogenated pyrimidines. The virus contained an RNA-directed DNA polymerase and the p30 structural protein. The rabbit virus RNA-directed DNA polymerase and p30 protein shared antigenic homologies with other mammalian type C oncornaviruses but appear to also possess unique antigenic determinants. Normal rabbit liver contained DNA homologous to a 3H-labeled complementary DNA transcript of the rabbit viral genome, indicating that type C viral genetic information is present in at least the WH/J strain of rabbits.
D000818:Animals; D002460:Cell Line; D000939:Epitopes; D007065:Idoxuridine / Q000494:pharmacology*; D008228:Lymphoma, Non-Hodgkin; D012328:RNA Viruses; D012194:RNA-Directed DNA Polymerase / Q000276:immunology / Q000378:metabolism; D011817:Rabbits; D012190:Retroviridae / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development*; D014779:Virus Replication / Q000187:drug effects*
D000939:Epitopes; D012194:RNA-Directed DNA Polymerase; D007065:Idoxuridine