Direct-acting mutagens in automobile exhaust.
Particulate matter in city air contains chemicals which are mutagenic in the Ames Salmonella typhimurium assay. In residential urban areas, the principal mutagens in air do not require liver enzymes to be activated. The source of these liver independent (direct-acting) mutagens may be automobile exhaust because (1) the mutagenic activities were correlated to the lead content or air, (2) the mutagens were found exhaust samples from automobiles and from an experimental CFR single-cylinder gasoline engine, and (3) these mutagens were not found in fuel or unused motor oil, but were found in used motor oil. The strain specificity and the fact that liver enzymes were not required for activation indicated that the exhaust and airborne mutagens were not unsubstituted polycyclic aromatic hydrocarbons (PAH), aromatic amines, alkylnitrosamines or aliphatic epoxides, peroxides and hydroepoxides. A number of nitro-substituted aromatic compounds are direct-acting mutagens in the Ames test, and it is possible that nitration of PAH in exhaust may form the compounds observed here. We synthesized 6-nitrobenzo [a] pyrene and found it to be a potent, direct-acting mutagen with activity comparable to that of benzo-[a] pyrene.
D000818:Animals; D001711:Biotransformation; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism; D009153:Mutagens; D009574:Nitro Compounds / Q000494:pharmacology; D011083:Polycyclic Compounds / Q000494:pharmacology; D051381:Rats; D012486:Salmonella typhimurium / Q000187:drug effects*; D001335:Vehicle Emissions / Q000633:toxicity*
D009153:Mutagens; D009574:Nitro Compounds; D011083:Polycyclic Compounds; D001335:Vehicle Emissions