Immunosuppressive factor(s) specific for L-glutamic acid50-L-tyrosine50 (GT). I. Production, characterization, and lack of H-2 restriction for activity in recipient strain.
The random synthetic copolymer of L-glutamic acid50-L-tyrosine50 (GT) fails to elicit a GT-specific antibody response in all inbred strains of mice tested. Preimmunization with GT specifically inhibits a GT-MBSA response in certain H2d,k,s, but not other, H-2a,b,q, nonresponder mice. This unresponsiveness is mediated by GT-specific suppressor T cells. Extracts prepared from lymphoid cells of GT-primed suppressor haplotype mice inhibit the development of primary GT-specific antibody responses to GT-MBSA in normal syngeneic mice. Nonsuppressor haplotype mice do not produce GT-specific suppressor factor. The GT-suppressive extract has affinity for antigen and a m.w. of less than 50,000 daltons, thus, resembling antigen-specific immunosuppressive factors already described. However, the GT-suppressive extract does not appear to have H-2 restrictions since it works across allogeneic barriers. Evidence is presented that two genes are required for factor-mediated suppression.
D000818:Animals; D000917:Antibody Formation; D002457:Cell Extracts / Q000276:immunology / Q000494:pharmacology; D000939:Epitopes; D005971:Glutamates / Q000276:immunology*; D006462:Hemolytic Plaque Technique; D006648:Histocompatibility; D007166:Immunosuppressive Agents; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D012710:Serum Albumin, Bovine / Q000276:immunology; D014443:Tyrosine / Q000276:immunology*
D002457:Cell Extracts; D000939:Epitopes; D005971:Glutamates; D007166:Immunosuppressive Agents; D012710:Serum Albumin, Bovine; D014443:Tyrosine