Biosynthesis of myelin and neurotoxic factors in the serum of multiple sclerosis patients.
The in vitro synthesis of myelin proteins has been studied by measuring the incorporation of [3H] lysine in developing rat brain slices. This incorporation system has been used to assay potentially gliotoxic and myelinolytic agents. A reduced incorporation of the labelled amino acid into myelin proteins occurs in the presence of anti-myelin anti-serum and anti-basic protein anti-serum. Diphtheria toxin has been found to inhibit the synthesis of myelin basic and proteolipid protein in the white matter slices of developing rats. Recent experiments with serum samples from multiple sclerosis patients in exacerbation suggest the presence of a factor which interferes with the synthesis of myelin in white matter slices.
D000818:Animals; D001921:Brain / Q000254:growth & development / Q000378:metabolism; D004167:Diphtheria Toxin / Q000494:pharmacology; D006801:Humans; D007518:Isoantibodies; D009103:Multiple Sclerosis / Q000378:metabolism*; D004676:Myelin Basic Protein / Q000276:immunology; D009185:Myelin Proteins / Q000096:biosynthesis*; D009186:Myelin Sheath / Q000254:growth & development* / Q000276:immunology; D009457:Neuroglia / Q000254:growth & development*; D009474:Neurons / Q000502:physiology; D009836:Oligodendroglia / Q000254:growth & development*; D051381:Rats
D004167:Diphtheria Toxin; D007518:Isoantibodies; D004676:Myelin Basic Protein; D009185:Myelin Proteins