Protease inhibitors reduce mitogen induced lymphocyte stimulation.
Proteolytic events might play a role during lymphocyte activation. Mouse spleen cells were therefore stimulated in serum-free cultures by PHA, ConA, LPS and dextran sulphate and the effect of various added protease inhibitors on [3H]-thymidine incorporation investigated. Both soybean inhibitor and Trasylol inhibited the response of the cells to all mitogens. The other inhibitors (antipain, leupeptin, ovomucoid, alpha-1 trypsin, alpha-2 macroglobulin) had little or no effect. The marked inhibitory effect of tosyl-lysine chloromethylketone could be neutralized by reduced glutathione, indicating an effect on intracellular glutathione rather than on proteases.
D000818:Animals; D003208:Concanavalin A / Q000494:pharmacology; D003911:Dextrans / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008934:Mitogens / Q000494:pharmacology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D011480:Protease Inhibitors / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology; D000511:alpha-Macroglobulins / Q000494:pharmacology
D003911:Dextrans; D008070:Lipopolysaccharides; D008934:Mitogens; D010835:Phytohemagglutinins; D011480:Protease Inhibitors; D000511:alpha-Macroglobulins; D003208:Concanavalin A