title: Ultrastructural study on the meiotic prophase nucleus of rat oocytes.
abstract: Rat oocytes in the meiotic prophase are studied by means of classical techniques of electron microscopy, preferential staining methods for DNA and RNA and specific enzymatic hydrolysis. The axial cores in leptotene and the lateral arms in the pachytene synaptonemal complex are composed by fibrils that keep a positive contrast after the application of the ethylenediaminetetraacetic acid staining method. They disappear with RNAse treatment, which reveals the presence of chromatin fibrils in the zone occupied by the cores. Preferential staining for DNA corroborates this evidence. Medial arm and lateral-medial fibrils are formed by ribonucleoproteic filaments that form bridges between pairing homologues in the zygotene. In the advanced pachytene stage, the RNA becomes scarce in these structures. No DNA can be detected either in the lateral-medial fibrils or in the medial arm. During diplotene the synaptonemal complex loses its individually and the synaptic space becomes wider and irregular. At the same time, loss of chromatin and a large increase of RNA-containing particles occur. These processes lead to the typical interphasic arrangement of nuclear components seen in the dictyate stage.
mesh_terms: D000818:Animals; D000831:Animals, Newborn / Q000033:anatomy & histology; D002462:Cell Membrane / Q000648:ultrastructure; D002467:Cell Nucleus / Q000648:ultrastructure; D002843:Chromatin / Q000648:ultrastructure; D004247:DNA / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism; D005260:Female; D008540:Meiosis; D009865:Oocytes / Q000378:metabolism / Q000648:ultrastructure*; D010063:Ovum / Q000648:ultrastructure*; D051381:Rats; D012260:Ribonucleases / Q000378:metabolism; D013194:Staining and Labeling; D013574:Synaptosomes / Q000648:ultrastructure; D015053:Zygote / Q000648:ultrastructure
chemical_list: D002843:Chromatin; D004247:DNA; D003851:Deoxyribonucleases; D012260:Ribonucleases