A cytofluorometric method of counting trypanosomes.
A rapid automated method of counting trypanosomes was developed using cytofluorometric methods. Organisms were vitally stained with acridine orange, and DEAE cellulose separated T. congolese were accurately counted at all concentrations. T. congolense in heparinized whole bovine blood were also counted accurately at levels greater than 1,000 per microliter. At lower levels, small fluorescent particles in whole blood interfered with the counts.
D000818:Animals; D001769:Blood / Q000469:parasitology; D002417:Cattle; D003636:DEAE-Cellulose; D005470:Fluorometry / Q000379:methods; D013194:Staining and Labeling; D014345:Trypanosoma / Q000302:isolation & purification*
D003636:DEAE-Cellulose