Computer assisted image analysis of Feulgen-stained cell nuclei from transitional cell carcinoma of the human urinary bladder.
Computer assisted image analysis was performed in Feulgen-stained cell nuclei from 117 transitional cell carcinomas of the human urinary bladder. The results were compared to corresponding findings from a control group consisting of 27 specimens from non-malignant urothelium. Generally, the mean nuclear area in carcinoma specimens was found to be increased as compared to the control group, both in diploid and non-diploid tumours. The concentration and the distribution of nuclear chromatin, and the relative area and content of non-condensed chromatin were dependent on nuclear DNA-content and nuclear area. These nuclear parameters did not give supplementary information which could be used in discriminating benign urothelial cell nuclei from malignant ones. A multiple regression analysis, performed for diploid and non-diploid tumours separately, did not show any significant correlation between the considered nuclear parameters and clinical stage of the tumours or the patient's survival.
D000328:Adult; D000368:Aged; D002295:Carcinoma, Transitional Cell / Q000648:ultrastructure*; D002467:Cell Nucleus / Q000648:ultrastructure*; D002843:Chromatin / Q000032:analysis; D003201:Computers; D003584:Cytological Techniques; D004273:DNA, Neoplasm / Q000032:analysis; D004171:Diploidy; D006801:Humans; D008875:Middle Aged; D011379:Prognosis; D012044:Regression Analysis; D013194:Staining and Labeling; D001749:Urinary Bladder Neoplasms / Q000648:ultrastructure*
D002843:Chromatin; D004273:DNA, Neoplasm