PBS-soluble substances with blood group activity from human epidermis and dermis.
Phosphate-buffered saline (PBS) soluble extracts of human epidermis and dermis from secretor donors contain products with blood group antigenic activity. The predominant blood group in a sample was the same as the red cell phenotype of the donor. H substance activity was present in all PBS-soluble products (from A, B and O donors). By gel filtration chromatography on a Bio-Gel A-1.5 m column; the epidermal and dermal PBS-soluble blood group substances fractionate in the void volume.
D001789:Blood Group Antigens; D002021:Buffers; D002850:Chromatography, Gel; D004817:Epidermis / Q000032:analysis* / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D005779:Immunodiffusion; D010710:Phosphates; D012867:Skin / Q000032:analysis* / Q000276:immunology; D012965:Sodium Chloride; D012995:Solubility
D001789:Blood Group Antigens; D002021:Buffers; D010710:Phosphates; D012965:Sodium Chloride