Coupling of gamma-globulin to microcrytstalline cellulose by periodate oxidation.
Anti-rabbit IgG sheep gamma-globulins were covalently coupled to periodate oxidized microcrystalline cellulose using the Schiff reaction. Optimal conditions (sodium m-periodate concentration and gamma-globulin amount) were studied measuring the ability of this solid-phase antibody to bind glucose oxidase-labeled rabbit IgG.
D000818:Animals; D000941:Antigens; D001666:Binding Sites, Antibody; D002482:Cellulose; D004305:Dose-Response Relationship, Drug; D010084:Oxidation-Reduction; D010504:Periodic Acid / Q000494:pharmacology*; D011817:Rabbits; D012756:Sheep; D005719:gamma-Globulins / Q000276:immunology*
D000941:Antigens; D005719:gamma-Globulins; D010504:Periodic Acid; D002482:Cellulose