Effect of pepsin pretreatment on pulse-cytophotometric DNA histograms.
Samples of mitotic L-cells were investigated after different preparation and staining procedures using the technique of pulse-cytophotometry. It is shown that most mitotic cells which should appear in the second peak of the DNA histogram are disintegrated or separated into halves by pepsin pretreatment. Hence, the designation 'G2 + M' for the second peak is not correct for this preparative method. This should be taken into account in cell kinetic investigations performed after pepsin pretreatment.
D003584:Cytological Techniques; D004247:DNA / Q000096:biosynthesis*; D004996:Ethidium; D005069:Evaluation Studies as Topic; D007739:L Cells / Q000378:metabolism; D008938:Mitosis / Q000187:drug effects*; D010434:Pepsin A / Q000494:pharmacology*; D010783:Photometry; D008926:Plicamycin; D012260:Ribonucleases / Q000494:pharmacology; D013194:Staining and Labeling
D004247:DNA; D012260:Ribonucleases; D010434:Pepsin A; D004996:Ethidium; D008926:Plicamycin