Use of immunoadsorbents for the study of antibody binding to sperm whale myoglobin and its synthetic antigenic sites.
Conditions for preparing immunoadsorbents of sperm-whale myoglobin and its five synthetic antigenic sites and for desorption of radiolabeled antibodies from the immunoadsorbents were studied. In immunoadsorbent titration studies, the sum of the amounts of antibodies bound in the plateau (maximum binding) by the adsorbents of the five sites accounted quantitatively for the entire (100%) antibody response to sperm-whale myoglobin.
D000818:Animals; D000906:Antibodies / Q000378:metabolism*; D000936:Antigen-Antibody Complex; D055598:Chemical Phenomena; D002621:Chemistry; D000939:Epitopes; D007164:Immunosorbents; D008297:Male; D009211:Myoglobin / Q000276:immunology*; D010455:Peptides / Q000302:isolation & purification; D014907:Whales
D000906:Antibodies; D000936:Antigen-Antibody Complex; D000939:Epitopes; D007164:Immunosorbents; D009211:Myoglobin; D010455:Peptides