Ag-staining of nucleolus organizer regions of chromosomes after A-,C-, G-, or R-banding procedures.
Metaphase chromosome preparations were made from leukocyte cultures of normal individuals. The cells were fixed in methanol:acetic acid (3:1 v/v), then dropped on cold, wet slides which were air-dried before storage at 4 degrees C. The slides were stained to identify the chromosomes by one of the following procedures: (1) Quinacrine. Slides were stained for 10 min in quinacrine mustard solution, rinsed in running tap water for 2 min, and mounted in Tris-maleat buffer, pH 5.6.
D000818:Animals; D002466:Cell Nucleolus / Q000648:ultrastructure*; D002875:Chromosomes / Q000648:ultrastructure*; D006801:Humans; D008938:Mitosis; D012834:Silver; D013194:Staining and Labeling
D012834:Silver