Isolation of reovirus-like agents from patients with Crohn's disease.
220 nm filtrates of intestinal resections from 6 of 10 patients with Crohn's disease produced a cytopathic effect in WI-38 tissue-culture monolayers, whereas controls had no effect. Characterisation of the virus responsible has been completed in 3 of the 6 positive isolates and indicates that it is an R.N.A. virus, 55-60 nm in diameter, heat, ether, and acid stable, and antigenically related to Nebraska calf-diarrhoea virus. It therefore belongs to the Reoviridae family.
D000956:Antigens, Viral; D003424:Crohn Disease / Q000382:microbiology*; D046508:Culture Techniques; D006801:Humans; D008854:Microscopy, Electron / Q000379:methods; D009500:Neutralization Tests; D012087:Reoviridae / Q000276:immunology / Q000302:isolation & purification* / Q000648:ultrastructure
D000956:Antigens, Viral