The relationship between cholera toxin receptor and the Thy-1 antigen determinants.
The aim of the present study was to determine the relationship of the Thy-1 antigenic determinants to the cell surface ganglioside GM1. Exposure of either thymus or bone marrow cells to Thy-1 alloantiserum partially inhibited the binding of [125I]cholera toxin (CT) by the cells. Xenoantibodies to Thy-1 antigens failed to affect the binding of [125I]CT to mouse thymus cells. In the reverse experiments, treatment of either thymus cells or brain homogenate with CT did not alter their capacity to absorb Thy-1 allo- and xenoantibodies. It is concluded that GM1 ganglioside does not constitute a Thy-1 antigenic determinant.
D000818:Animals; D002772:Cholera Toxin / Q000276:immunology* / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D000939:Epitopes; D007106:Immune Sera; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D011817:Rabbits; D051381:Rats; D011955:Receptors, Drug / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D000939:Epitopes; D007106:Immune Sera; D011955:Receptors, Drug; D002772:Cholera Toxin