Antigenic determinants of C3 and C4 complement components on washed erythrocytes from normal persons.
Well-washed erythrocytes from normal persons were agglutinated by antisera to C3, C3d, and C4, and this agglutination was specifically inhibited by the corresponding C3 or C4 protein. C3 and C4 antigenic determinants were present on the red blood cells of freshly shed blood promptly anticoagulated with EDTA, heparin, ACD, or CPD, and no significant changes in degree of agglutination were observed on storage of EDTA or CPD blood for two weeks at 4 C. Marked differences in degree of agglutination by anti-C3, anti-C3d, and anti-C4 were observed when erythrocytes of 16 normal persons were assayed, and significant correlations were obtained when the quantitative results with any two antisera were compared. Anti-C3c did not agglutinate erythrocytes from normal persons, suggesting that the C3 antigens detected on normal cells are carried by the C3d fragment. To avoid significant agglutination of the erythrocytes from some normal persons, very dilute preparations of anti-C3, -C3d, and -C4 had to be used for instrumented diagnostic direct antiglobulin tests. Stronger reagents could be used for indirect antiglobulin tests when the result of a suitable control could be subtracted.
D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology; D001793:Blood Preservation; D003176:Complement C3; D003181:Complement C4; D000939:Epitopes; D004912:Erythrocytes / Q000276:immunology*; D006385:Hemagglutination Inhibition Tests; D006386:Hemagglutination Tests; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D011817:Rabbits
D000925:Anticoagulants; D003176:Complement C3; D003181:Complement C4; D000939:Epitopes; D007106:Immune Sera