title: Pretreatment of hamster cells with phenethyl alcohol alters cell surface glycoproteins and inhibits vesicular stomatitis virus growth.
abstract: Chinese hamster ovary cells cultured in the presence of phenethyl alcohol exhibit obvious changes in cell surface galactose and galactosamine glycoproteins as determined by the galactose-oxidase[3H]borohydride technique and SDS gel electrophoresis. Cells pretreated with phenethyl alcohol (drug was removed before infection) were not as effective as hosts for vesicular stomatitis virus as untreated cultures. A minimum pretreatment time with 0.1% phenethyl alcohol of about 8 h was required before a reduction in virus growth was observed. It is proposed that phenethyl alcohol pretreatment as outlined in this report leads to a modification of the host cellular membrane resulting in the inhibition of virus replication.
mesh_terms: D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects*; D002470:Cell Survival; D000431:Ethanol / Q000031:analogs & derivatives*; D006023:Glycoproteins; D008565:Membrane Proteins; D010626:Phenylethyl Alcohol / Q000494:pharmacology*; D011132:Polyribosomes / Q000187:drug effects; D014721:Vesicular stomatitis Indiana virus / Q000254:growth & development*
chemical_list: D006023:Glycoproteins; D008565:Membrane Proteins; D000431:Ethanol; D010626:Phenylethyl Alcohol