A technique for the simultaneous staining of both nucleolar organizer regions and kinetochores of human chromosomes with silver.
Pretreatment of human metaphase chromosomes with NaOH at a pH of 8.5, followed by staining with silver nitrate, differentially stains both the nucleolar organizer regions on the 10 acrocentric chromosomes as well as the kinetochore centers on all 46 chromosomes.
D002466:Cell Nucleolus / Q000648:ultrastructure; D002877:Chromosomes, Human / Q000648:ultrastructure*; D006801:Humans; D008677:Metaphase; D008938:Mitosis; D012835:Silver Nitrate; D012972:Sodium Hydroxide; D013194:Staining and Labeling / Q000379:methods*
D012972:Sodium Hydroxide; D012835:Silver Nitrate