A technique for demonstrating of dehydrogenase activities in Epon-embedded, semithin sections of the nervous tissue.
Experiments were performed aiming at establishing optimal conditions for demonstrating the activity of dehydrogenases in Epon-embedded, semithin sections. Small fragments of the nervous tissue were incubated 1, 4, 10 and 24 h 37, 20 and 4 degrees C in the presence of NBT or TC NBT. The incubated fragments were embedded in Epon and semithin sections prepared thereof. The activities of succinate dehydrogenase and of alpha-glycerol dehydrogenase was evaluated. The experiments have shown that semithin, Epon-embedded sections may be used for demonstration and cytological localization of respiratory enzymes. Various experimental conditions were tried. Optimal results obtained when unfixed tissue fragments were incubated for 24 h at 4 degrees C.
D000818:Animals; D002525:Cerebellar Cortex / Q000201:enzymology*; D006651:Histocytochemistry; D008526:Medulla Oblongata / Q000201:enzymology*; D010904:Pituitary Gland, Posterior / Q000201:enzymology*; D051381:Rats; D013116:Spinal Cord / Q000201:enzymology*; D013194:Staining and Labeling; D013385:Succinate Dehydrogenase / Q000032:analysis*
D013385:Succinate Dehydrogenase