Human glomerular basement membrane. Heterogeneity of antigenic determinants.
Human glomerular basement membrane was solubilized by digestion with proteolytic enzymes and immunoreactive components were quantitated and characterized by using rabbit antibodies raised against the particulate membrane. A number of antigens were demonstrated but they did not separate on gel filtration. However, two antigenic components in a collagenase digest of the membrane could be separated and isolated by Sepharose 6B chromatography. Chemical characterization suggests that both fragments are noncollagenous glycopeptides (molecular weights approx. 1,000,000 and 60,000--200,000, respectively).
D000596:Amino Acids / Q000032:analysis; D001485:Basement Membrane / Q000032:analysis / Q000276:immunology*; D002241:Carbohydrates / Q000032:analysis; D000939:Epitopes; D006801:Humans; D007678:Kidney Glomerulus / Q000276:immunology*; D003012:Microbial Collagenase / Q000378:metabolism; D010446:Peptide Fragments / Q000032:analysis / Q000276:immunology; D010447:Peptide Hydrolases / Q000378:metabolism
D000596:Amino Acids; D002241:Carbohydrates; D000939:Epitopes; D010446:Peptide Fragments; D010447:Peptide Hydrolases; D003012:Microbial Collagenase