Enhancement of the DNA synthetic response of antigen-primed lymph node cells by splenic promoter cells: characterization of the splenic promoter cells.
Rabbits were immunized in the footpad with diphtheria toxoid in complete Freund's adjuvant. At various times after immunization, cells harvested from the spleen, the draining (immune) and the opposing (control) lymph nodes (LN) were assayed for their proliferative response to incorporate tritiated thymidine upon exposure to the priming antigen. Although the immune LN cells responded by a substantial incorporation of thymidine, cells from the control LN and the spleen either did not respond or responded feebly. An enhancement in the response of the immune LN cells was observed when they were cultured in the presence of nonresponsive spleen cells. Pretreatment of spleen cells with mitomycin C did not abolish the enhancement. This suggests that the target cells which respond to the antigen are derived from the immune LN whereas the promoter cells which enhance the response are present in the spleen. The removal of adhering cells by glass wool columns and of Ig-bearing cells by anti-Ig immunoabsorbent columns from the spleen did not reduce the enhancing capacity of the nonadhering cells. Conversely, the killing of splenic T cells by specific heterologous antiserum directed against rabbit thymus lymphocyte antigen abolished the enhancement. Thus, the promoter cell which facilitates the enhancement has been characterized as a nonadherent, splenic T cell.
D000818:Animals; D000941:Antigens; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis*; D004168:Diphtheria Toxoid / Q000494:pharmacology; D004306:Dose-Response Relationship, Immunologic; D000939:Epitopes; D005260:Female; D007700:Kinetics; D008198:Lymph Nodes / Q000276:immunology*; D008213:Lymphocyte Activation; D011817:Rabbits; D013154:Spleen / Q000276:immunology*; D013950:Thymus Gland / Q000276:immunology
D000941:Antigens; D004168:Diphtheria Toxoid; D000939:Epitopes; D004247:DNA