Extensive sequence homology at the 3'-termini of the four RNAs of cucumber mosaic virus.
The sequences of 270 residues from the 3'-termini of the four RNAs of cucumber mosaic virus have been determined by copying the in vitro polyadenylated RNAs with reverse transcriptase using d(pT8G) as primer and the 2',3'-dideoxynucleoside 5'-triphosphates as specific chain terminators. The terminal sequences of RNAs 3 and 4 were identical; this was expected since hybridization data has shown that the sequence of RNA 4 was present at the 3'-end of RNA 3 (Gould and Symons (1978) Eur. J. Biochem. 91, 269-278). The first 138 residues of RNAs 1 and 2 were identical to those of RNAs 3 and 4 except for one residue in RNA 1 and three residues in RNA 2. From residue 139 to 270 from the 3'-terminus, RNAs 1 and 2 showed, relative to RNAs 3 and 4, a non-homologous region of 33 residues, a homologous region of 40 residues, a partially homologous region of 14 residues which probably extended to about residue 300. There were 11 residues different between RNAs 1 and 2.
D001482:Base Composition; D001483:Base Sequence; D009029:Mosaic Viruses / Q000032:analysis*; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D009843:Oligoribonucleotides / Q000032:analysis; D011061:Poly A; D012367:RNA, Viral / Q000302:isolation & purification; D012194:RNA-Directed DNA Polymerase
D009843:Oligoribonucleotides; D012367:RNA, Viral; D011061:Poly A; D012194:RNA-Directed DNA Polymerase