Preferential synthesis of IgE reaginic antibodies in rats immunized with alum-adsorbed antigens.
The reaginic antibody response to alum-precipitated ovalbumin (OA) and the dialyzed water-soluble extracts of ragweed (DWSR) and Alternaria tenuis (DWST) in several strains of rats appeared to be wholly an IgE response. There was no evidence of a heat-stable (IgGa) antibody to OA, DWSR and DWST in the sera of the rats immunized with these antigens suspended in alum. Wistar-Furth and Lew inbred and hooded outbred rats produced comparable amounts of reaginic antibody after immunization with DWST, but BN inbred rats failed to generate a reaginic response to this antigen. The amount of antigen-induced histamine release from rat peritoneal mast cells did not always correlate with the level of circulating IgE-specific antibody.
D000327:Adsorption; D000528:Alternaria / Q000276:immunology; D000534:Alum Compounds / Q000494:pharmacology*; D000535:Aluminum / Q000494:pharmacology*; D000818:Animals; D000917:Antibody Formation; D000941:Antigens / Q000276:immunology*; D005260:Female; D006636:Histamine Release; D007106:Immune Sera / Q000494:pharmacology; D007073:Immunoglobulin E / Q000096:biosynthesis*; D008297:Male; D010323:Passive Cutaneous Anaphylaxis; D010936:Plant Extracts / Q000276:immunology; D051381:Rats; D011914:Rats, Inbred BN; D011917:Rats, Inbred Lew; D011920:Rats, Inbred WF; D011935:Reagins / Q000096:biosynthesis*
D000534:Alum Compounds; D000941:Antigens; D007106:Immune Sera; D010936:Plant Extracts; D011935:Reagins; D007073:Immunoglobulin E; D000535:Aluminum