Biosynthesis of enzyme-bound formylvaline and formylvalylglycine. A possible initiation complex for gramicidin A biosynthesis.
A partially purified fraction (Component I) which catalyzed the ATP-PPi exchange reaction dependent only on valine and glycine among the five constituent amino acids of gramicidin A was obtained from crude extracts of Bacillus brevis ATCC 8185, which produces both gramicidin A and tyrocidines. This fraction was found to bind valine and glycine as thioesters. The addition of a formylTHFA synthesizing system to Component I brought about the formation of Component I-bound formylvaline and formylvalylglycine.
D001407:Bacillus / Q000378:metabolism; D004151:Dipeptides / Q000096:biosynthesis; D005561:Formates / Q000378:metabolism; D005998:Glycine / Q000378:metabolism; D006096:Gramicidin / Q000096:biosynthesis*; D013763:Tetrahydrofolates / Q000096:biosynthesis; D014633:Valine / Q000031:analogs & derivatives / Q000096:biosynthesis
D004151:Dipeptides; D005561:Formates; D013763:Tetrahydrofolates; D006096:Gramicidin; C014564:formylvalylglycine; C014563:formylvaline; D014633:Valine; D005998:Glycine