RNA-dependent DNA polymerase associated with equine infectious anemia virus.
Equine infectious anemia (EIAV) is shown to have an associated RNA-instructed DNA polymerase similar in its cofactor requirements and reaction conditions to the RNA tumor virus DNA polymerases. Demonstrating this DNA polymerase activity requires a critical concentration of a nonionic detergent, all four deoxyribonucleoside triphosphates, and a divalent metal ion. The reaction is sensitive to RNase, and a substantial fraction of the FNA synthesized is complementary to viral RNA. The detection of a complex of tritium-labeled polymerase product DNA-template RNA, which sedimented at 60S to 70S, provided evidence that EIAV contains high-molecular-weight RNA. These results, obtained with both virus propagated in cell culture and virus from the serum of an experimentally infected horse, indicate that EIAV may properly be considered a member of the family Retroviridae. They may also be pertinent to the mechanism(s) of viral persistence and periodic recrudescence of disease in chronically infected horses.
D004279:DNA, Viral / Q000096:biosynthesis; D003609:Dactinomycin / Q000494:pharmacology; D004860:Infectious Anemia Virus, Equine / Q000032:analysis / Q000201:enzymology* / Q000378:metabolism; D008274:Magnesium / Q000378:metabolism; D008345:Manganese / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D009711:Nucleotides / Q000378:metabolism; D012367:RNA, Viral / Q000032:analysis / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D012260:Ribonucleases / Q000378:metabolism; D013501:Surface-Active Agents / Q000494:pharmacology; D013698:Templates, Genetic
D004279:DNA, Viral; D009711:Nucleotides; D012367:RNA, Viral; D013501:Surface-Active Agents; D003609:Dactinomycin; D008345:Manganese; D012194:RNA-Directed DNA Polymerase; D012260:Ribonucleases; D008274:Magnesium