Anti B and T cell activity of antiserum to spleen cells of athymic (nu/nu) mice: evidence for surface antigenic differences between functionally distinct subclasses of T and B lymphocytes.
A rabbit antiserum obtained by immunization with spleen cells of nu/nu mice, known to be deficient in thymus and T cells but not in T cell precursor, possesses two different specificities. In addition to activity against bone marrow-derived lymphocytes (B cells), as revealed by inhibition of antibody forming cells and myeloma cells, the antiserum (Ra-nu/nu) has strong activity against cytotoxic T cells but not against helper T cells. Differences in sensitivity to antiserum treatment between anatomically and functionally distinct B cells point to surface antigenic differences among subclasses of B cells.
D000042:Absorption; D000818:Animals; D000918:Antibody Specificity; D000961:Antilymphocyte Serum / Q000494:pharmacology*; D001202:Ascitic Fluid / Q000166:cytology; D001402:B-Lymphocytes / Q000276:immunology*; D002462:Cell Membrane / Q000276:immunology; D002469:Cell Separation; D003601:Cytotoxicity Tests, Immunologic; D000939:Epitopes; D005260:Female; D006462:Hemolytic Plaque Technique; D007111:Immunity, Cellular; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D008297:Male; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D013154:Spleen / Q000166:cytology*; D013601:T-Lymphocytes / Q000276:immunology*
D000961:Antilymphocyte Serum; D000939:Epitopes; D007074:Immunoglobulin G; D007075:Immunoglobulin M