title: [Ultrastructure of Mycoplasma sp. demonstrated by phosphotungstic acid-chromic acid pH 0,4].
abstract: Ultrastructural observation of Mycoplasma sp. revealed by the phosphotungstic acid-cromic acid, pH 0,4. Arq. Inst. Biol., São Paulo, 45(3) :169-176, 1978. Cells of IB-RS-2 (clone C-26-3) swine line were examined under electron microscopy, employing the differential coloration with PTA-ACr 5 %, pH 0,4, during 30 minutes, at 37 degrees C. Through this method we could observe the intracitoplasmatic forms, electronically dense, rather pleomorfic, with sizes varying between 60nm and 640nm and a unitary membrane of 90A similar to the extracellular forms described as Mycoplasma sp.
mesh_terms: D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D004396:Coloring Agents / Q000494:pharmacology; D007668:Kidney; D008854:Microscopy, Electron; D009174:Mycoplasma / Q000648:ultrastructure*; D013194:Staining and Labeling; D013552:Swine
chemical_list: D004396:Coloring Agents
vernacular_title: Ultraestrutura de Micoplasma sp. revelada pelo ácido fosfotúngstico-ácido crômico pH 0,4.