title: The anticlastogenic effect of various combinations of cysteamine, AET, HCT, and amino acids on chromosome damage by trenimon and bleomycin in human lymphocytes in vitro.
abstract: The protective activity of the combined application of anticlastogens against the chromosome-damaging action of Trenimon and bleomycin was studied by analyzing more than 32000 metaphases from cultures of human peripheral lymphocytes. Screening tests with the combinations cysteine/cysteamine/AET, cysteine/methionine/asparagine, cysteine/serine/HCT, and AET/HCT, with Trenimon as clastogen, in no case revealed a hyperadditive (synergistic) effect. From the results of detailed analyses of the action of the combination AET/HCT it was concluded that the (nonsynergistic) anticlastogenic effects observed were induced due to intracellular biologic mechanism, and not due to a reaction in the culture fluid between clastogens and anticlastogens. Although the observations gained with Trenimon or bleomycin differed in some respects, the anticlastogens apparently act via a mechanism at least common to AET and HCT, i.e., they manifest their effect in lymphocyte cultures by a limited interaction with the process of aberration formation, rather than by influencing repair processes, which are blocked by caffeine.
mesh_terms: D000477:Alkylating Agents / Q000037:antagonists & inhibitors; D000603:Amino Acids, Sulfur / Q000494:pharmacology*; D001224:Aspartic Acid / Q000494:pharmacology; D001761:Bleomycin / Q000037:antagonists & inhibitors; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000187:drug effects*; D003543:Cysteamine / Q000494:pharmacology*; D003545:Cysteine / Q000494:pharmacology; D007783:Lactones / Q000494:pharmacology; D008715:Methionine / Q000494:pharmacology; D009153:Mutagens / Q000037:antagonists & inhibitors*; D012694:Serine / Q000494:pharmacology; D014228:Triaziquone / Q000494:pharmacology*; D000338:beta-Aminoethyl Isothiourea / Q000494:pharmacology*
chemical_list: D000477:Alkylating Agents; D000603:Amino Acids, Sulfur; D007783:Lactones; D009153:Mutagens; D001761:Bleomycin; D000338:beta-Aminoethyl Isothiourea; D001224:Aspartic Acid; D012694:Serine; D003543:Cysteamine; D008715:Methionine; D014228:Triaziquone; D003545:Cysteine