BUdR as a tracer of the possible mutagenic activity of Pb++ in human lymphocyte cultures.
The method of SCE was used as an indicator of the possible mutagenic activity of lead acetate in in vitro cultures of human lymphocytes. After determining the optimal concentration of BUdR, the cultures were treated with increasing doses of the metal. The negative results obtained with this method led us to examine other parameters, in particular the classical cytogenetic aberrations.
D001973:Bromodeoxyuridine / Q000378:metabolism; D002453:Cell Cycle; D002478:Cells, Cultured; D002842:Chromatids / Q000187:drug effects*; D002869:Chromosome Aberrations; D004247:DNA / Q000096:biosynthesis; D006801:Humans; D007854:Lead / Q000633:toxicity*; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D009153:Mutagens; D013194:Staining and Labeling
D009153:Mutagens; D007854:Lead; D004247:DNA; D001973:Bromodeoxyuridine