[The effect of storage and short term cultivation the proliferation of mouse bone marrow stem cells].
Suspension of stored and short-term cultured murine bone marrow cells was i. v. administered to lethally irradiated mice. We demonstrated that the cells still after 72 h of storing and 24 h of culture in liquid medium were capable of proliferation and on day 9 following transplantation could produce colony forming units in the spleens of lethally irradiated mice. We established the relative participation of individual types of cells in the recovery of haemopoiesis in the spleens.
D000818:Animals; D016026:Bone Marrow Transplantation; D002455:Cell Division; D002470:Cell Survival; D002478:Cells, Cultured; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D051379:Mice; D011828:Radiation Chimera; D013154:Spleen / Q000166:cytology; D014021:Tissue Preservation
Der Einfluss von Lagerung und Kurzzeitkultivierung auf die Repopulationsfähigkeit von Stammzellen aus Mäuseknochenmark.