A rapid assay for neuraminidase. The detection of two differences in activity associated with virus transformation.
Neuraminidase (acylneuraminyl hydrolase, EC 3.2.1.18) activity in fibroblast homogenates was measured by a rapid and simple assay with a synthetic substrate. The activity of neuraminidase in virus transformed hamster fibroblasts was increased over the normal counterpart. In addition, the differential activity seen using the synthetic substrate and fetuin made it possible to detect an enzyme activity hitherto not described. The advantages of this assay for metabolic screening are discussed.
D002460:Cell Line; D002472:Cell Transformation, Viral; D007700:Kinetics; D009439:Neuraminidase / Q000378:metabolism*; D009975:Orthomyxoviridae / Q000201:enzymology; D013379:Substrate Specificity; D014734:Vibrio cholerae / Q000201:enzymology; D000509:alpha-Fetoproteins
D000509:alpha-Fetoproteins; D009439:Neuraminidase