Cellular basis of regulation of expression of idiotype. I. T-suppressor cells specific for MOPC 460 idiotype regulate the expression of cells secreting anti-TNP antibodies bearing 460 idiotype.
An idiotype of the dinitrophenyl-binding myeloma protein MOPC 460 was expressed on a small but significant proportion of anti-TNP antibodies which appeared after in vivo or in vitro immunization of BALB/c mice with three T-independent TNP antigens. In vitro experiments show that the depletion of T cells before culture increased significantly the number of plaques secreting anti-TNP antibodies bearing MOPC 460 idiotype (460Id). T cells from BALB/c mice, but not from C.B20 mice, exhibit this suppressor activity. Plate-binding experiments indicate that the suppressive action of the T-lymphocyte population depends on a cell which can bind to MOPC 460 myeloma protein. The possible role of these normally occurring, idiotype-specific T cells on expression of 460Id in the anti-TNP antibody response of BALB/c mice is discussed.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D000917:Antibody Formation; D000939:Epitopes; D006241:Haptens; D007111:Immunity, Cellular; D007130:Immunoglobulin Idiotypes; D007165:Immunosuppression Therapy; D051379:Mice; D008815:Mice, Inbred Strains; D009194:Myeloma Proteins / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014301:Trinitrobenzenes / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D000939:Epitopes; D006241:Haptens; D007130:Immunoglobulin Idiotypes; D009194:Myeloma Proteins; D014301:Trinitrobenzenes