Expression of an early, nonstructural antigen of herpes simplex virus in cell transformed in vitro by herpes simplex virus.
Hyperimmune rabbit antiserum to an early, nonstructural herpes simplex virus type 2 (HSV-2)-induced polypeptide (VP143) reacted in immunofluorescence tests with a variety of cell lines transformed by HSV-2. Cytoplasmic fluorescence was observed in 10 to 50% of HSV-2-transformed cells, whereas no fluorescence was observed in cells transformed by other oncogenic DNA viruses or by a chemical carcinogen. VP143-specific reactivity could be absorbed from anti-VP143 serum with HSV-2-transformed cells but not with cells transformed by other agents. When HSV-2-transformed cells were synchronized in mitosis and examined at various times postmitosis for VP143-specific fluorescence, the expression of VP143 was shown to be cell cycle dependent.
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000276:immunology; D000260:Adenoviruses, Human / Q000276:immunology; D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D003593:Cytoplasm / Q000276:immunology; D004273:DNA, Neoplasm / Q000096:biosynthesis; D000939:Epitopes; D005455:Fluorescent Antibody Technique; D008938:Mitosis; D010455:Peptides / Q000032:analysis / Q000276:immunology*; D013539:Simian virus 40 / Q000276:immunology; D018139:Simplexvirus / Q000276:immunology*
D000956:Antigens, Viral; D004273:DNA, Neoplasm; D000939:Epitopes; D010455:Peptides; D015127:9,10-Dimethyl-1,2-benzanthracene