Isolabeling of the long arm of the human Y chromosome demonstrated by the FPG technique.
Isolabeling segments were found in the distal region of the long arm of Y chromosomes derived from human leukocytes grown through two replication cycles in medium containing BrdU and stained by the FPG technique. Three main types of Y chromosome staining patterns were demonstrated: I-Y chromosome with typical SCD, II-Y chromosome with weakly stained distal regions of long arms (isolabeling segments), III-Y chromosome with both terminal regions displaying SCD interrupted by one isolabeled segment. The existence of different types of Y chromosome staining patterns was explained on the basis of the previously described hypothesis of unequal distribution of thymine residues between two DNA polynucleotide chains in the distal part of the long arms of human Y chromosomes.
D006690:Bisbenzimidazole; D006801:Humans; D007962:Leukocytes / Q000648:ultrastructure; D008297:Male; D012730:Sex Chromosomes / Q000648:ultrastructure*; D013194:Staining and Labeling / Q000379:methods*; D014466:Ultraviolet Rays; D014998:Y Chromosome / Q000528:radiation effects / Q000648:ultrastructure*
D006690:Bisbenzimidazole