Ia-bearing bone marrow-cultured macrophages induce antigen-specific helper T cells for antibody synthesis.
In bone marrow cell (BMC) cultures supplemented with colony-stimulating factor (CSF), accessory cells develop that are capable of inducing specific helper T cells. These accessory cells become effective after 4 days in culture and can be found not only in the adherent but also in the nonadherent cell population. On the other hand, very few accessory cells with helper cell-inducing capacity are obtained in BMC cultures without CSF. The active BMC-derived cell type has been shown to carry Ia surface antigen, since pretreatment with anti-Ia serum and complement abolished the capacity of these cells to function like macrophages in helper T cell induction. Moreover, the appearance of functional accessory cells in these cultures coincided with the presence of Ia-bearing cells.
D000818:Animals; D000917:Antibody Formation; D001853:Bone Marrow / Q000276:immunology*; D002448:Cell Adhesion; D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000494:pharmacology; D000939:Epitopes; D006649:Histocompatibility Antigens / Q000276:immunology; D008264:Macrophages / Q000276:immunology*; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D009504:Neutrophils / Q000276:immunology; D010586:Phagocytes / Q000201:enzymology; D013601:T-Lymphocytes / Q000276:immunology*; D013997:Time Factors
D003115:Colony-Stimulating Factors; D000939:Epitopes; D006649:Histocompatibility Antigens