Application of quantitative high-performance thin-layer chromatography in the antibiotic industry.
For the in-process control of antibiotic fermentations and for routine assays of samples for scaling up and for pure products, quantitative high-performance thin-layer chromatography (HPTLC) can be used with advantage. Rapid chromatography on high-performance layers, combined with an automatic spraying device for exact derivatization on the plate and precise computation of the calibration line within an automatic measurement and evaluation, represents a new, inexpensive analysis system. There are only 2 min of labour time (one fifth of that required in thin-layer chromatography) required for one sample and the total analysis time varies from 3 to 9 min (one third to one quarter of that required in thin-layer chromatography) based on one plate with 12 samples. The 95% confidence limits (N = 10) range between 0.5 and 3.0%.
D000900:Anti-Bacterial Agents / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004345:Drug Industry; D005285:Fermentation; D006096:Gramicidin; D008722:Methods
D000900:Anti-Bacterial Agents; D006096:Gramicidin