Radioimmunoassay of the insulin receptor: a new probe of receptor structure and function.
A sensitive and specific radioimmunoassay for the insulin receptor has been developed employing receptor autoantibodies from the serum of a patient with insulin-resistant diabetes. The assay detects insulin binding sites at concentrations as low as 0.1 nanomolar; distinguishes between receptors originating from human placental membranes, human lymphoblastoid cells, and mouse liver membranes; and measures the receptor independently of its binding function. Down-regulation, or loss of binding after exposure to insulin, is associated with loss of immunoreactive receptor.
D000818:Animals; D000937:Antigen-Antibody Reactions; D001665:Binding Sites; D001666:Binding Sites, Antibody; D000939:Epitopes; D005260:Female; D006801:Humans; D008099:Liver / Q000032:analysis; D008214:Lymphocytes / Q000032:analysis; D051379:Mice; D010920:Placenta / Q000032:analysis; D011247:Pregnancy; D011863:Radioimmunoassay / Q000379:methods; D011972:Receptor, Insulin / Q000032:analysis / Q000276:immunology*; D012995:Solubility
D000939:Epitopes; D011972:Receptor, Insulin