Surface antigens of rat liver epithelial cells grown in medium containing foetal bovine serum.
Cultured rat liver cells induced a strong antibody response in syngeneic rats, directed against foetal calf serum components which were incorporated into the liver cell surface from the cell-culture medium. This antibody could be removed by absorption with liver cells or glutaraldehyde-fixed foetal calf serum. It is possible that the antigenic cross-reactivity observed in earlier studies with cultured cells treated with carcinogens could be due to this foetal calf serum component.
D000818:Animals; D000917:Antibody Formation; D000954:Antigens, Surface / Q000032:analysis*; D002478:Cells, Cultured; D003470:Culture Media; D004848:Epithelium / Q000276:immunology; D005312:Fetal Blood; D008099:Liver / Q000276:immunology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D000509:alpha-Fetoproteins
D000954:Antigens, Surface; D003470:Culture Media; D000509:alpha-Fetoproteins