A new preparation method for several histopathological examinations on a single block.
A new method of specimen preparation is described permitting several studies such as routine staining, histochemistry, enzyme histochemistry, immunohistochemistry, and electron microscopy on a single block of biopsy specimens. Tissues are immersed in the fixative, which primarily stabilizes carbohydrate moieties, and embedded in the mixture of JB-4, methylmethacrylate and divinylbenzene. The resin is polymerized at 4 C. Thin sections (1-2 microns) are obtained with a sliding microtome, and ultrathin sections (60-90 millimicrons) with a ultramicrotome. The sections are stained directly with various conventional procedures without removing the embedding resin. This preparation method offers a potentially useful tool for histopathological studies on biopsy specimens.
D000818:Animals; D001706:Biopsy; D005455:Fluorescent Antibody Technique; D006651:Histocytochemistry; D006652:Histological Techniques; D006801:Humans; D008854:Microscopy, Electron; D051381:Rats; D013048:Specimen Handling / Q000379:methods; D013194:Staining and Labeling