Preparative electrophoresis of human lymphocytes. II. Use of non-immunoglobulin bearing lymphocytes obtained by electrophoretic levitation for the elicitation of a rabbit anti-human "T-cell" serum.
By intradermal injection (in Freund's complete adjuvant) of rabbits with only a few million human T-lymphocytes, obtained by preparative electrophoresis, an anti-human "T-cell" serum can be obtained. Although the antiserum is best used in a fairly concentrated form, its anti-human "T-cell" specificity (by immunofluorescence and E-rosetting) appears excellent, without the need to resort to absorption with various unwanted antigens. There may however, also be other specificities in the antiserum, as it also reacts with human granulocytes.
D000818:Animals; D000961:Antilymphocyte Serum / Q000302:isolation & purification*; D002469:Cell Separation; D004592:Electrophoresis, Starch Gel; D000939:Epitopes; D004912:Erythrocytes / Q000276:immunology; D006098:Granulocytes / Q000276:immunology; D006801:Humans; D008214:Lymphocytes / Q000276:immunology; D011817:Rabbits; D011947:Receptors, Antigen, B-Cell; D013601:T-Lymphocytes / Q000276:immunology*
D000961:Antilymphocyte Serum; D000939:Epitopes; D011947:Receptors, Antigen, B-Cell