[Isolation, from anti-dinitrophenyl sera taken at the beginning of immunization, of a protein combining with the hapten and which is not related to immunoglobulins].
A protein, not antigenically related to the immunoglobulins, has been isolated by affinity chromatography from the serum of rabbits immunized with dinitrophenylated human gamma-globulin. Its concentration in the serum reaches maximum during the first three days which follow the injection. On "Sephadex G-200", it is eluted after the ribonuclease. Its electrophoretic mobility is slower than that of the IgG.
D000818:Animals; D002846:Chromatography, Affinity; D004136:Dinitrobenzenes / Q000276:immunology*; D000939:Epitopes; D006241:Haptens; D007106:Immune Sera / Q000032:analysis*; D007114:Immunization; D007136:Immunoglobulins; D009578:Nitrobenzenes / Q000276:immunology*; D011485:Protein Binding; D011506:Proteins / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D011817:Rabbits; D013997:Time Factors
D004136:Dinitrobenzenes; D000939:Epitopes; D006241:Haptens; D007106:Immune Sera; D007136:Immunoglobulins; D009578:Nitrobenzenes; D011506:Proteins
Isolement, à partir de sérums anti-dinitrophényl prélevés en début d'immunisation, d'une protéine se combinant au haptène et qui n'est pas apparentée aux immunoglobulines