title: Mitogenic activity of Actinomyces viscosus. I. Effects on murine B and T lymphocytes, and partial characterization.
abstract: Actinomyces viscosus homogenate (AVIS) contins substance(s) which cause spleen cells from conventional and germfree mice to undergo increased DNA synthesis. This mitogenic effect is primarily on B cells since spleen cells from nude mice or T-depleted spleen cells from conventional mice respond as strongly as conventional (T + B) spleen cells. Mouse thymocytes do not respond mitogenically to AVIS. It is unlikely that the mitogenic acitivity is due to the presence of LPS, since A. viscosus is Gram-positive and is not known to have an LPS cell wall component. Also, AVIS is not inactivated by polymyxin B, as are some preparations of LPS, and C3H/HeJ mouse splenocytes respond strongly to AVIS but not to LPS. The activity is heat stable, is not lost upon dialysis, and is not affected by lysozyme. Mitogenic activity is partially lost when AVIS is digested with nonspecific bacterial protease or treated with metaperiodate. Sodium hydroxide treatment completely abolishes mitogenic activity. Actinomycotic lesions are characterized by a long-tern inflammatory response involving a dense plasma cell infiltrate. We suggest that B cell mitogens form Actinomyces may play a role in the elicitation of the plasma cell component of these lesions.
mesh_terms: D000190:Actinomyces / Q000276:immunology*; D000468:Alkalies / Q000494:pharmacology; D000818:Animals; D000942:Antigens, Bacterial / Q000008:administration & dosage; D001402:B-Lymphocytes / Q000276:immunology*; D002454:Cell Differentiation; D000939:Epitopes; D005260:Female; D005856:Germ-Free Life; D006358:Hot Temperature; D066298:In Vitro Techniques; D008070:Lipopolysaccharides / Q000276:immunology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000378:metabolism; D008222:Lymphokines / Q000096:biosynthesis; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D008934:Mitogens; D009113:Muramidase / Q000494:pharmacology; D010447:Peptide Hydrolases / Q000494:pharmacology; D010504:Periodic Acid / Q000494:pharmacology; D010950:Plasma Cells / Q000276:immunology; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000468:Alkalies; D000942:Antigens, Bacterial; D000939:Epitopes; D008070:Lipopolysaccharides; D008222:Lymphokines; D008934:Mitogens; D010504:Periodic Acid; D009113:Muramidase; D010447:Peptide Hydrolases