Synaptonemal complexes of mouse and human pachytene chromosomes visualized by silver staining in air-dried preparations.
A new method is described which allows light microscopy visualization of the synaptonemal complexes in primary spermatocytes. The conventional acetic acid-alcohol fixed and air-dried preparations can be used for the modified silver staining technique. Several hundred pachytene spermatocytes from a single mouse testis or human testicular biopsy can be examined. The prospective utilization of the present method is briefly discussed.
D000818:Animals; D002877:Chromosomes, Human / Q000648:ultrastructure*; D006651:Histocytochemistry; D006801:Humans; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D013090:Spermatocytes / Q000648:ultrastructure*; D013094:Spermatozoa / Q000648:ultrastructure*; D013194:Staining and Labeling