title: Human glomerular basement membrane. Heterogeneity of antigenic determinants.
abstract: Human glomerular basement membrane was solubilized by digestion with proteolytic enzymes and immunoreactive components were quantitated and characterized by using rabbit antibodies raised against the particulate membrane. A number of antigens were demonstrated but they did not separate on gel filtration. However, two antigenic components in a collagenase digest of the membrane could be separated and isolated by Sepharose 6B chromatography. Chemical characterization suggests that both fragments are noncollagenous glycopeptides (molecular weights approx. 1,000,000 and 60,000--200,000, respectively).
mesh_terms: D000596:Amino Acids / Q000032:analysis; D001485:Basement Membrane / Q000032:analysis / Q000276:immunology*; D002241:Carbohydrates / Q000032:analysis; D000939:Epitopes; D006801:Humans; D007678:Kidney Glomerulus / Q000276:immunology*; D003012:Microbial Collagenase / Q000378:metabolism; D010446:Peptide Fragments / Q000032:analysis / Q000276:immunology; D010447:Peptide Hydrolases / Q000378:metabolism
chemical_list: D000596:Amino Acids; D002241:Carbohydrates; D000939:Epitopes; D010446:Peptide Fragments; D010447:Peptide Hydrolases; D003012:Microbial Collagenase