Spontaneous histamine secretion from leukocytes in the presence of strontium.
Basophil leukocytes from human blood secrete histamine in the absence of a membrane stimulus when incubated in a medium containing Sr++, 1 to 10 mM. Spontaneous histamine secretion in the presence of Sr++ is inhibited by La+++, 1 to 1000 nM and 2 deoxy-D-glucose, 30 to 300 microM. Spontaneous secretion in the presence of Sr++ increases with increasing pH in the range 6.5 to 8.5. Agents which cause a rise of intracellular cyclic AMP level increase the spontaneous secretion in 194th presence of Sr++. The results suggest that spontaneous histamine release is a secretory response of the cells, and evidence is provided for a dual role of cyclic AMP in the control of histamine secretion.
D001491:Basophils / Q000187:drug effects / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism / Q000494:pharmacology; D002462:Cell Membrane / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism; D003847:Deoxyglucose / Q000494:pharmacology; D004347:Drug Interactions; D006636:Histamine Release / Q000187:drug effects*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007811:Lanthanum / Q000494:pharmacology; D009994:Osmolar Concentration; D013324:Strontium / Q000494:pharmacology*; D013997:Time Factors
D007811:Lanthanum; D003847:Deoxyglucose; D000242:Cyclic AMP; D002118:Calcium; D013324:Strontium