A new staining technique for microfilariae.
A rapid whole mount staining method is described to identify and differentiate microfilariae without elaborate processing. A single solution combining Hoyer's mounting medium and hematoxylin stain facilitates light microscopic examination of nuclei and sheaths of microfilariae. The new technique stains microfilariae adequately in three to seven minutes at 60--64 C making the method preferable to conventional methods that may take as long as 45 to 60 minutes. Lantern heat may be used to heat slides in rural areas with good results.
D000818:Animals; D002467:Cell Nucleus / Q000032:analysis; D003593:Cytoplasm / Q000032:analysis; D005370:Filarioidea / Q000033:anatomy & histology*; D006416:Hematoxylin; D008842:Microfilariae / Q000033:anatomy & histology*; D013194:Staining and Labeling / Q000379:methods*
D006416:Hematoxylin