title: A coated-tube radioimmunoassay (FRC-RIA) for hepatitis B surface antigen.
abstract: A sandwich-type solid phase radioimmunoassay, FRC-RIA (Finnish Red Cross-RIA) for hepatitis B surface antigen (HBsAg) is described. Polystyrene test tubes coated with sheep anti-HBs gamma-globulin serve as the solid phase. The technique is specific and has a sensitivity of about 1 ng/ml for the subtypes ad and ay of HBsAg when corresponding subtype-specific antibodies are used as reagents. The total incubation time can be shortened from over 20 h to 3 h, with almost equal sensitivity, when the incubation temperature is raised from room temperature to 45 degrees C. The test is economical in reagents and is applicable to routine use.
mesh_terms: D000914:Antibodies, Viral; D000918:Antibody Specificity; D004306:Dose-Response Relationship, Immunologic; D006514:Hepatitis B Surface Antigens; D006801:Humans; D011309:Preservation, Biological; D011863:Radioimmunoassay / Q000379:methods; D013696:Temperature; D013997:Time Factors; D005719:gamma-Globulins
chemical_list: D000914:Antibodies, Viral; D006514:Hepatitis B Surface Antigens; D005719:gamma-Globulins