Transfer of delayed hypersensitivity in mice to microbial antigens with dialyzable transfer factor.
Dialyzable Lawrence-type transfer factor was prepared from the spleen cells of CF1 mice inoculated with Coccidioides immitis- and Candida albicans-killed vaccines and with live Mycobacterium tuberculosis vaccine (BCG). These preparations were shown to transfer antigen-specific cell-mediated immunity to naive mice, as measured by the delayed skin test and footpad-swelling methods. Reactivity could be demonstrated when the test antigens were given 24 h after the transfer factor, but not when they were given simultaneously. Coccidioides-specific transfer factor was shown to be sensitive to Pronase and resistant to trypsin and ribonuclease. A preparation of BCG transfer factor was sensitive to snake venom phosphodiesterase.
D000818:Animals; D000942:Antigens, Bacterial; D000946:Antigens, Fungal; D001500:BCG Vaccine; D002175:Candida / Q000276:immunology; D003045:Coccidioides / Q000276:immunology; D000939:Epitopes; D005260:Female; D006968:Hypersensitivity, Delayed; D007116:Immunization, Passive; D051379:Mice; D009163:Mycobacterium bovis / Q000276:immunology; D013154:Spleen / Q000276:immunology; D014165:Transfer Factor
D000942:Antigens, Bacterial; D000946:Antigens, Fungal; D001500:BCG Vaccine; D000939:Epitopes; D014165:Transfer Factor