The L1210 leukemia cell bears a B lymphocyte specific, non-H-2 linked alloantigen.
The L1210 murine lymphoblast cell line possessed a B lymphocyte-specific alloantigen which was detected with C57BL/Ks anti-L1210 serum. The antigen was found on splenic B lymphocytes but not on thymocytes, T lymphocytes, or erythrocytes. It was present on only 7% of bone marrow cells. The reactivity of C57BL/Ks anti-DBA/2-spleen serum was indistinguishable from that of the anti L1210 serum, confirming that the antigen was a normal component of B lymphocytes of the CBA/2 mouse. The strain distribution pattern of the antigen detected by the C57BL/Ks anti-L1210 serum indicated that this alloantigen was not an allele of the H-2K, H-2D, Mls, or Ig loci. Genetic analysis indicated that the antigen was inherited as a single, Mendelian dominant trait. Segregation analysis of (B10.BR X DBA/2)F1 X AKR/J offspring indicated that this B cell marker was not linked to geneslambdacoding for H-2.31 or Ly 4.2. The C57BL/Ks anti-L1210 serum identified a new polymorphic genetic locus, the product of which was B lymphocyte specific.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D002874:Chromosome Mapping; D003601:Cytotoxicity Tests, Immunologic; D004306:Dose-Response Relationship, Immunologic; D000939:Epitopes; D004912:Erythrocytes / Q000276:immunology; D008040:Genetic Linkage; D006649:Histocompatibility Antigens / Q000032:analysis*; D007106:Immune Sera / Q000032:analysis; D007939:Leukemia L1210 / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D013045:Species Specificity; D013154:Spleen / Q000166:cytology
D000939:Epitopes; D006649:Histocompatibility Antigens; D007106:Immune Sera