VH and L-chain allotype determinants of rabbit IgG and IgA estimated by a semi-automatic, modified Farr-type radioimmunoassay;.
A sensitive radioimmunoassay for rabbit immunoglobulin allotypes has been developed. Separation of antibody-bound and free antigen was accomplished by salt precipitation with ammonium sulfate, followed by collection and wash on glass fiber filters by means of a semi-automatic cell harvester. The efficiency of the collection was increased by the addition of nonionic detergent which was salted out together with the precipitated reagents. The presentation of the a 1 allotype, located in the VH region, was found to be different on IgG and IgA molecules.
D000818:Animals; D001667:Binding, Competitive; D011232:Chemical Precipitation; D000939:Epitopes; D007106:Immune Sera / Q000032:analysis; D007125:Immunogenetics; D007070:Immunoglobulin A / Q000276:immunology*; D007126:Immunoglobulin Allotypes; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G / Q000276:immunology*; D011817:Rabbits; D011863:Radioimmunoassay
D000939:Epitopes; D007106:Immune Sera; D007070:Immunoglobulin A; D007126:Immunoglobulin Allotypes; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G