Ia-bearing cells promote the concanavalin A mitogenic response of Ia-negative T cells.
Nylon wool column-purified T cell populations depleted of Ia-bearing cells do not respond to the T cell mitogen concanavalin A (Con A). The response of this population can be restored by admixture of untreated nylon wool-nonadherent T cells. These promotor cells express Thy-1, are not sensitive to mitomycin C treatment and cannot be replaced with adherent spleen cells. Experiments using the T6 chromosome marker demonstrate that Ia-depleted populations respond to ConA only in the presence of the Ia-positive population.
D000818:Animals; D002469:Cell Separation; D003208:Concanavalin A / Q000494:pharmacology*; D000939:Epitopes; D007111:Immunity, Cellular; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008808:Mice, Inbred CBA; D008937:Mitomycins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology*
D000939:Epitopes; D008937:Mitomycins; D003208:Concanavalin A