A critical examination of possible fractionations of human DNA according to base composition.
Human DNA has been fractionated according to base composition by sedimentation equilibrium in an HgCl2/Cs2SO4 density gradient, followed by sedimentation equilibrium in an actinomycin/cesium formate density gradient. The fractions of different base composition resulting from this procedure were subsequently analyzed by sedimentation equilibrium in CsCl, DNA renaturation kinetics, and electron microscopy. All fractions contain similar kinetic classes of repeated DNA sequences as judged by renaturation studies. Short (300 nucleotides) interspersed repeated sequences are found in all fractions with no noticeable enrichment for these sequences in any fraction. Repeated sequences from fractions of different base composition are partially able to cross-hybridize, demonstrating that nearly identical repeated sequences occur in molecules of different base composition. These findings are critically compared to reports of successful density gradient fractionations of different human DNA sequence classes.
D002499:Centrifugation, Density Gradient; D002586:Cesium; D004247:DNA / Q000302:isolation & purification*; D004276:DNA, Satellite; D003609:Dactinomycin; D003854:Deoxyribonucleotides / Q000032:analysis; D006801:Humans; D009695:Nucleic Acid Renaturation
D004276:DNA, Satellite; D003854:Deoxyribonucleotides; D003609:Dactinomycin; D002586:Cesium; D004247:DNA