The expression of Ia antigenic determinants on macrophages required for the in vitro antibody response.
A subpopulation of antigen-presenting macrophages required for an in vitro antibody response to burro erythrocytes was deleted by pretreating the splenic macrophages with anti-Ia serum and complement (C). The in vitro response of the macrophage depleted T-B cell population could not be restored by the addition of macrophages resistant to anti-Ia antibodies and C (Ia-). The response of Ia- macrophages and the macrophage-depleted T-B cells was only reconstituted by the addition of Ia+ macrophages. Macrophages pretreated with anti-Ia antibodies restricted to react with determinants of one I subregion could not support the in vitro antibody response when added to cultures whose macrophages were pretreated with anti-Ia serum and C specific for the I-J subregion. These results confirmed that Ia determinants of the I-A, the I-E, and the I-C subregions were all expressed on the I-J+ macrophage required for an in vitro antibody response.
D000818:Animals; D000917:Antibody Formation; D002448:Cell Adhesion; D002469:Cell Separation; D004306:Dose-Response Relationship, Immunologic; D000939:Epitopes; D006462:Hemolytic Plaque Technique; D007106:Immune Sera / Q000494:pharmacology; D008264:Macrophages / Q000276:immunology*; D008285:Major Histocompatibility Complex; D051379:Mice; D008805:Mice, Inbred A; D008806:Mice, Inbred AKR; D010527:Perissodactyla; D014176:Protein Biosynthesis; D011817:Rabbits
D000939:Epitopes; D007106:Immune Sera