[Purification of solubilized fucosyltransferase from lamb brain using ethylagarose gel].
The glycoprotein: fucosyl-transferase of the cerebral hemispheres, assayed with desialylated fetuin as exogenous acceptor, was the most active of the protein: glycosyltransferases tested in the brain. The addition of Titron X-100 to the membrane suspension, followed by high speed centrifugation, led to a solubilization of the enzyme. The use of hydrophobic chromatography on ethylagarose gave a good purification of this solubilized fucosyl-transferase, whose homogeneity has been shown by Ultrogel AcA 22, DEAE-cellulose chromatography and disc electrophoresis.
D000818:Animals; D001921:Brain / Q000201:enzymology*; D002847:Chromatography, Agarose / Q000379:methods; D006023:Glycoproteins; D006154:Guanosine Diphosphate Fucose; D006602:Hexosyltransferases / Q000302:isolation & purification*; D012995:Solubility; D000509:alpha-Fetoproteins
D006023:Glycoproteins; D000509:alpha-Fetoproteins; D006154:Guanosine Diphosphate Fucose; D006602:Hexosyltransferases
Purification de la fucosyl-transférase solubilisée du cerveau d'Agneau : utilisation d'un gel d'éthylagarose.