title: In vitro synthesis of infectious DNA of murine leukaemia virus.
abstract: DNA synthesised in vitro by purified virions of murine leukaemia virus is infectious. Neither RNA nor protein is required for infectivity. Transfection with reverse trancriptase product shows a single-hit dose response and results in the production of complete, infectious virus.
mesh_terms: D002460:Cell Line; D002474:Cell-Free System; D004277:DNA, Single-Stranded / Q000378:metabolism; D004279:DNA, Viral / Q000096:biosynthesis*; D003609:Dactinomycin / Q000494:pharmacology; D008970:Molecular Weight; D008979:Moloney murine leukemia virus / Q000235:genetics*; D012367:RNA, Viral / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D013329:Structure-Activity Relationship; D014162:Transfection; D014779:Virus Replication
chemical_list: D004277:DNA, Single-Stranded; D004279:DNA, Viral; D012367:RNA, Viral; D003609:Dactinomycin; D012194:RNA-Directed DNA Polymerase