A radioimmune assay for human cupro-zinc superoxide dismutase and its application to erythrocytes.
A competition radioimmunoassay for human superoxide dismutase (SOD-1) is described. Radioiodinated SOD-1 is incubated with unlabeled competitor and limiting antibody, then immune complexes are isolated using Staphylococcus aureus. The assay is sensitive (detecting 5 ng enzyme) and reproducible. Average values for SOD-1 in lysates of erythrocytes from normal individuals is 854 +/- 100 ng/mg hemoglobin and that of patients with trisomy-21 is 1313 +/- 110 ng/mg hemoglobin.
D000818:Animals; D000906:Antibodies; D000918:Antibody Specificity; D001667:Binding, Competitive; D004314:Down Syndrome / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D007124:Immunoenzyme Techniques; D011863:Radioimmunoassay; D013482:Superoxide Dismutase / Q000032:analysis*
D000906:Antibodies; D000939:Epitopes; D007106:Immune Sera; D013482:Superoxide Dismutase