Isoelectric focusing of gamma globulins in cerebrospinal fluid from patients with multiple sclerosis.
The technique of isoelectric focusing has been adapted for rapid clinical analysis for globulins in cerebrospinal fluid with use of commercially prepared horizontal-slab acrylamide gels. The globulin fraction is concentrated by ammonium sulfate precipitation, which allows more of the relevant protein to be applied, use of a wider range of total protein concentrations, and higher resolution than is true for previously described methods. Critical variables include a constant concentration and volume of IgG, a constant low temperature of the acrylamide gel, and sensitive staining with Coomassie Brilliant Blue G-250. The apparatus used is adaptable for other electrophoretic procedures in the clinical laboratory, and the use of commercially prepared gel slabs is more convenient, more reproducible, and requires less time than other methods.
D006801:Humans; D007525:Isoelectric Focusing / Q000379:methods; D009103:Multiple Sclerosis / Q000134:cerebrospinal fluid*; D005719:gamma-Globulins / Q000134:cerebrospinal fluid*
D005719:gamma-Globulins