Quantitation of mast cell heparin by flow cytofluorometry.
Mast cells can be automatically identified in a mixed cell population by flow cytofluorometry after Berberine sulphate staining. Volume specific counts of the total number of cells and number of mast cells, as well as frequency distributions of fluorescence intensities of mast cells, based on a large number of cells, can be rapidly obtained. Results obtained by microscope fluorometry of cells identified by phase contrast microscopy showviously published results it may be inferred that the fluorescence intensity of individual mast cells is proportional to mast cell heparin content. The automated cell counts correlated very well with manual hemocytometer counts. Both cell counts and the determination of mean mast cell fluorescence showed excellent reproducibility.
D000818:Animals; D001599:Berberine; D002448:Cell Adhesion; D002449:Cell Aggregation; D002452:Cell Count; D002469:Cell Separation; D006493:Heparin / Q000032:analysis*; D008297:Male; D008407:Mast Cells / Q000032:analysis*; D008722:Methods; D008856:Microscopy, Fluorescence; D051381:Rats; D013050:Spectrometry, Fluorescence; D013194:Staining and Labeling
D001599:Berberine; D006493:Heparin