The influence of serum content in the medium on the survival of human bone marrow in suspension in vitro.
Samples of human bone marrow cells from patients with various diseases were cultivated in vitro by means of a simple stationary suspension method. Medium Eagle MEM with the addition of allogeneic serum and borosilicate glass were used. The cells survived significantly longer in samples with 50 per cent of serum than in samples with 30 per cent of serum only. Monocytes showed the longest survival (max. 95 days) in cultures with 50 per cent of serum. Myelocytes and segmented neutrophils as well as normoblasts survived till 45th day and plasmocytes till 66th day. Mitoses in monocytes were found till 50th day. Moreover so called "satellitosis" of plasmacytes around a macrophage was observed in cases with reactive plasmacytosis.
D001769:Blood; D001854:Bone Marrow Cells; D002470:Cell Survival; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods*; D006801:Humans; D008938:Mitosis; D012397:Rosette Formation
D003470:Culture Media