A new method for degradation of the protein part of glycoproteins: isolation of the carbohydrate chains of asialofetuin.
A new method has been developed for degrading the protein part of several glycoproteins, whilst leaving the carbohydrate portion virtually intact apart from partial degradation at the reducing end. The method is based upon stabilization of the glycosidic linkages of the sugar residues by trifluoroacetyl groups and subsequent cleavage of the peptide bonds by transamidation. The two reactions are carried out in a mixture of trifluoroacetic anhydride and trifluoroacetic acid. After O- and N-detrifluoroacetylation, the carbohydrate portion can be isolated and re-N-acetylated. The applicability of the method is demonstrated by the isolation from asialofetuin of the carbohydrate chains that are attached by N- and O-glycosyl links.
D002241:Carbohydrates / Q000032:analysis; D006027:Glycosides / Q000032:analysis; D013058:Mass Spectrometry / Q000379:methods; D012794:Sialic Acids; D000509:alpha-Fetoproteins
D002241:Carbohydrates; D006027:Glycosides; D012794:Sialic Acids; D000509:alpha-Fetoproteins