Spatial orientation and distribution of antigens within human glomerular basement membrane.
Antibodies to GBM have a linear staining pattern along the GBM by immunofluorescence. In this study, we have examined the distribution of human GBM antigens using Goodpasture antibody and heterologous rabbit anti-human GBM antibody. Goodpasture sera and Goodpasture antibody eluted from diseased kidneys reacted as a single linear component within the GBM. Rabbit anti-human GBM was shown to react along distinctly separate zones of the inner and outer aspects of the GBM; the central portion of the GBM seemed to be nonreactive with this antibody. In comparison, Goodpasture antibodies reacted along the inner zone of the GBM but at a locus external to that reacting with heterologous rabbit anti-GBM antibody. These observations of linear binding of antibody to different sites along the GBM suggest a spatial organization of antigen not previously observed.
D019867:Anti-Glomerular Basement Membrane Disease / Q000276:immunology* / Q000473:pathology; D000906:Antibodies / Q000008:administration & dosage; D000941:Antigens; D001485:Basement Membrane / Q000276:immunology* / Q000648:ultrastructure; D001665:Binding Sites; D000939:Epitopes; D005455:Fluorescent Antibody Technique; D006801:Humans; D007678:Kidney Glomerulus / Q000276:immunology* / Q000648:ultrastructure; D010477:Perfusion
D000906:Antibodies; D000941:Antigens; D000939:Epitopes