Effect of various culturing conditions of Acholeplasma laidlawii on the yield and properties of the cells and cell membrane.
Acholeplasma laidlawii strain B cell cultured in complex medium have an optimal temperature of growth at 31 degrees. Maximal growth at this temperature is 80% higher than at 37 degrees. By stirring of the culture, the yield of the cell mass of 4 liter-cultures increases by 200%. With these modifications the yield of cell membranes increases 3--4 times with respect to that of static culturing at 37 degrees. Membranes obtained from better aerated cultures have a lower flavin and carotenoid content. Membranes obtained from agitated cultures show a higher content of the more insoluble membrane-core proteins. It is suggested that these proteins play a role in membrane and cell adhesiveness.
D000128:Acholeplasma laidlawii / Q000032:analysis / Q000254:growth & development*; D000332:Aerobiosis; D001426:Bacterial Proteins / Q000032:analysis; D002338:Carotenoids / Q000032:analysis; D002462:Cell Membrane / Q000032:analysis; D003470:Culture Media; D005415:Flavins / Q000032:analysis; D013696:Temperature
D001426:Bacterial Proteins; D003470:Culture Media; D005415:Flavins; D002338:Carotenoids