The relationship between DNA replication and chromosome structure.
The results obtained by acridine orange staining of chromosomes, after BrdU treatment, during one or two cell cycles, are described. The alterations of chromosome structure do not depend only on BrdU incorporation into DNA. Some other mechanisms are necessarily involved, and it is postulated that they are disturbances of protein-DNA association, occurring in G1 and in S- or  G2-phase.  The aspect of metaphase chromosomes then appears as the result of several metabolic steps, all occurring during interphase.
D000166:Acridines; D001973:Bromodeoxyuridine / Q000494:pharmacology*; D002842:Chromatids / Q000187:drug effects; D002875:Chromosomes / Q000187:drug effects*; D004261:DNA Replication; D006801:Humans; D008938:Mitosis; D013194:Staining and Labeling
D000166:Acridines; D001973:Bromodeoxyuridine