Specific binding of T lymphocytes to macrophages. V. The role of Ia antigens on Mphi in the binding.
The effect of anti-Ia alloantiserum on the capacity of selected peritoneal exudate lymphocytes (selected PEL) to bind to antigen-pulsed F1 (responder x nonresponder) macrophages was investigated. With the use of selected PEL for antigens under Ir gene control, it was shown that anti-Ia serum to the responder haplotype blocked adherence of selected PEL to antigen-pulsed macrophages whereas anti-Ia serum to the nonresponder haplotype did not. The target cell of the anti-Ia alloantiserum appeared to be the macrophage because anti-13 Ia in contrast to anti-2 Ia did not inhibit binding of F1 (2 x 13) DNP-GL selected PEL to DNP-GL pulsed strain-2 Mphi (responder strain). Taken together with previous experiments that indicate that an antibody to the native protein antigen employed is unable to block specific binding, the present results suggest that T cells may recognize fragments of exogenous antigen in association with Ia molecules.
D000818:Animals; D001665:Binding Sites; D004136:Dinitrobenzenes / Q000276:immunology; D000939:Epitopes; D005260:Female; D005971:Glutamates / Q000276:immunology; D006168:Guinea Pigs; D006238:Haploidy; D006649:Histocompatibility Antigens / Q000276:immunology*; D007106:Immune Sera / Q000494:pharmacology; D007518:Isoantibodies; D008214:Lymphocytes / Q000276:immunology; D008264:Macrophages / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D004136:Dinitrobenzenes; D000939:Epitopes; D005971:Glutamates; D006649:Histocompatibility Antigens; D007106:Immune Sera; D007518:Isoantibodies