Cultivation of fresh and frozen mouse bone marrow. - Application of methyl cellulose.
The culture of cells of both fresh and frozen mouse bone marrow on methyl cellulose (MC) was approached. We used 1.5%-concentration of MC and proved the stem cells of fresh and frozen mouse bone marrow to proliferate and form haemopoietic colonies on MC. We established that the freezing process did not significantly decrease the proliferative capacity of CFU-C stem cells.
D000818:Animals; D001853:Bone Marrow; D002470:Cell Survival; D003114:Colony-Forming Units Assay; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods*; D005260:Female; D005615:Freezing; D008747:Methylcellulose; D051379:Mice
D003470:Culture Media; D008747:Methylcellulose