[Use of the indirect rat mast cell degranulation reaction in vitro for characterization of the specific activity of non-infectious allergens].
The test of indirect degranulation of rat mast cells was applied to the study of the specific activity of 16 series of noninfectious allergens from domestic dust (3 series) from hotel dust (7 series) and from the pollen of fescue (6 series). The concentration of protein nitrogen in the allergens from the domestic and hotel dust constituted to 10 000 PNU, and in the allergen from the pollen of fescue--125 000, 50 000, 15 000 and 7 000 PNU. In studying the specific activity of domestic allergens use was made of the sera of patients suffering from bronchial asthma with a marked allergy to domestic dust sera of patients with pollinoses with a marked allergy to fescue pollen was applied to the study of the specific activity of pollen allergen. For the assessment of the indirect degranulation test in mast cells of rats an index of mast cell degranulation of rats (IMCDR) was employed. It was shown that the IMCDR values were equal to 0.46, 0.58, 0.62 in using the domestic dust and in the case of the hotel dust - from 0.17 to 0.28. The mean values for the group of preparations from the domestic dust were 2.5 times greater than for the group of preparations for the hotel dust and were 0.55 and 0.22, respectively. For the allergen from the fescue pollen the IMCDR values varied from 1.23 to 0.16, and increased with the rise of the protein nitrogen concentration (PNU). Additional studies demonstrated that the specific activity of the domestic dust allergen with the crude sera and with those preserved in frozen condition for 3 to 6 months was almost the same. The results obtained permit to draw a conclusion that the method of indirect degranulation of rat mast cells with trizing of commercial batches of infectious allergens.
D000485:Allergens / Q000592:standards*; D000818:Animals; D001249:Asthma / Q000276:immunology; D003594:Cytoplasmic Granules / Q000276:immunology; D004391:Dust; D000939:Epitopes; D006967:Hypersensitivity / Q000175:diagnosis*; D008407:Mast Cells / Q000276:immunology* / Q000648:ultrastructure; D011058:Pollen; D051381:Rats; D006255:Rhinitis, Allergic, Seasonal / Q000175:diagnosis; D012698:Serologic Tests
D000485:Allergens; D004391:Dust; D000939:Epitopes
Primenenie reaktsii nepriamoĭ degranuliatsii tuchnykh kletok krys in vitro dlia kharakteristiki spetsificheskoĭ aktivnosti neinfektsionnykh allergenov