[Ultrastructural identification of the nucleolus organizer by the silver staining technic].
The nucleolus organizer regions can be selectively stained in metaphase chromosome preparations by the Goodpasture and Bloom's technique which was adapted to electron microscopy analysis of cells during interphase. Using this technique, a selective accumulation of silver grains was observed over nucleolus light areas. This selective accumulation allows the identification of the interphase fibrillar centers as the nucleolus organizer regions. Ultrastructural relationships between fibrillar centers and dense fibrillar component are discussed.
D002466:Cell Nucleolus / Q000648:ultrastructure*; D002875:Chromosomes / Q000648:ultrastructure; D006801:Humans; D066298:In Vitro Techniques; D008677:Metaphase; D008854:Microscopy, Electron; D012834:Silver; D013194:Staining and Labeling
D012834:Silver
Identification ultrastructurale de l'organisateur nucléolaire par la technique à l'argent.