Effect of intraventricular administration of anti-somatostatin gamma-globulin on the lethal dose-50 of strychnine and pentobarbital in rats.
Effects of intraventricular injection of sheep anti-somatostatin gamma-globulin (anti-SSG) on strychnine-induced seizures, strychnine LD50, and pentobarbital LD50 were examined in male rats under light ether anesthesia. Ten microliters of anti-SSG given 2 h earlier significantly decreased the duration of strychnine-induced seizures as compared with that in the control rats pretreated with normal sheep gamma-globulin (NSG). This effect of anti-SSG seemed to be specific, as there was no difference in seizure duration between sheep anti-LHRH gamma-globulin (anti-LHRHG)- and NSG-pretreated rats. Survival rates in anti-SSG-pretreated rats after injection of strychnine and pentobarbital were significantly larger (P less than 0.01 and P less than 0.05, respectively) than those in the control rats receiving NSG. The administration of anti-SSG resulted in 26.7% and 22.9% increases in the LD50 of strychnine and pentobarbital, respectively. These results indicate that endogenous somatostatin in the cerebrospinal fluids and/or the periventricular tissue nodulates the response of the central nervous system to strychnine and pentobarbital in rats.
D000818:Animals; D007276:Injections, Intraventricular; D007928:Lethal Dose 50; D008297:Male; D010424:Pentobarbital / Q000633:toxicity*; D051381:Rats; D012640:Seizures / Q000139:chemically induced; D013004:Somatostatin / Q000276:immunology*; D013331:Strychnine / Q000633:toxicity*; D005719:gamma-Globulins / Q000494:pharmacology*
D005719:gamma-Globulins; D013004:Somatostatin; D013331:Strychnine; D010424:Pentobarbital