Effect of sera from cystic fibrosis homozygotes and heterozygotes on glucose metabolism in Vero cells.
The effect on intracellular production of carbon dioxide from glucose of sera from cystic fibrosis (C.F.) homozygotes and heterozygotes was determined in an established cell line (Vero cell) by a double-blind assay. CO2 production was reduced when cells were incubated with an ammonium-sulphate-precipitated fraction of sera from C.F. homozygotes and heterozygotes but not by a similar fraction from sera of healthy donors.
D000818:Animals; D002245:Carbon Dioxide; D002460:Cell Line; D003550:Cystic Fibrosis / Q000097:blood* / Q000235:genetics; D005947:Glucose / Q000378:metabolism*; D000882:Haplorhini; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D007668:Kidney
D002245:Carbon Dioxide; D005947:Glucose