The use of glutaraldehyde-conjugated horseradish peroxidase-bovine serum albumin in the visualization of concanavalin A binding to tissue sections of human colonic tumor.
A method is reported for the preparation of glutaraldehyde cross-linked horseradish peroxidase conjugates for the visualization of concanavalin A binding to frozen sections. The binding of concanavalin A to tissue sections of human colonic tumor is used to compare staining by horseradish peroxidase conjugates and monomers. Without decreasing specificity, the use of progressively larger molecular weight conjugates provides progressively greater intensity of staining of the epithelial components of colonic carcinomas and normal colons.
D000447:Aldehydes; D003110:Colonic Neoplasms / Q000378:metabolism; D003208:Concanavalin A / Q000378:metabolism; D005976:Glutaral; D006735:Horseradish Peroxidase; D006801:Humans; D066298:In Vitro Techniques; D008970:Molecular Weight; D011485:Protein Binding; D012710:Serum Albumin, Bovine; D013194:Staining and Labeling
D000447:Aldehydes; D003208:Concanavalin A; D012710:Serum Albumin, Bovine; D006735:Horseradish Peroxidase; D005976:Glutaral