title: [Light and dark Purkinje cells (light microscopic and microautoradiographic studies].
abstract: The authors deal with the phenomenon of dualism of pale and dark ganglion cells in general, the phenomenon which, since FLEMMING (1882), still remains an actual problem of neurohistology. They deal with Purkinje cells from a special aspect with the aim to demonstrate the dualism through various staining methods. They directed their attention also to the question of the influence of perfusion and immersion fixation and length of staining with luxol-fast-blue on the production of luxol-positive (chromophilic) Purkinje cells. The authors have found that these methodologic circumstances do not influence the production of luxol-positive Purkinje cells, so it can be hardly spoken about an artifact. Examinations with the labelled leucine have shown that the increased metabolic activity can be observed in those Purkinje cells which in HE-picture are seen as pale ones. In the dark Purkinje cells leucine granulations are located on the surface of plasmatic membrane and they follow to some distance the main dendrite of those cells. The microautoradiographic method evidences for the increased leucine metabolism of the pale Purkinje cells as well.
mesh_terms: D000818:Animals; D001345:Autoradiography; D002531:Cerebellum / Q000166:cytology*; D005404:Fixatives; D007930:Leucine / Q000378:metabolism; D008297:Male; D011689:Purkinje Cells / Q000166:cytology* / Q000378:metabolism; D051381:Rats; D013194:Staining and Labeling
chemical_list: D005404:Fixatives; D007930:Leucine
vernacular_title: Uber helle und dunkie Purkinje-Zellen (Lichtmikroskopische und mikroautoradiographische Untersuchungen.