title: Superior sensitivity of conjugates of horseradish peroxidase with wheat germ agglutinin for studies of retrograde axonal transport.
abstract: We have compared the retrograde axonal transport of horseradish peroxidase (HRP), to the retrograde transport of HRP conjugated with wheat germ agglutinin (WGA). Morphometric studies have shown that WGA-HRP conjugates were 40 times more sensitive than free HRP, in the tracing of retrograde connections from the rat submandibular gland to the superior cervical ganglion. Also, WGA-HRP was more sensitive than free HRP in the tracing of retrograde connections from the rat tongue to the hypoglossal nucleus. Our findings with WGA-HRP are consistent with the observations by Schwab et al. who reported (-125I) WGA is a highly sensitive retrograde tracer (Brain Research 152:145, 1978 (22)).
mesh_terms: D000818:Animals; D001370:Axonal Transport; D005727:Ganglia, Spinal / Q000378:metabolism; D006735:Horseradish Peroxidase / Q000378:metabolism; D007002:Hypoglossal Nerve / Q000378:metabolism; D037102:Lectins / Q000378:metabolism; D008297:Male; D010544:Peroxidases; D037121:Plant Lectins; D051381:Rats; D013363:Submandibular Gland / Q000378:metabolism; D014059:Tongue / Q000378:metabolism; D014908:Triticum
chemical_list: D037102:Lectins; D037121:Plant Lectins; D006735:Horseradish Peroxidase; D010544:Peroxidases