Liposomes as model membrane systems for immune attack. I. Transfer of antigenic determinants to lymphocyte membranes after interactions with hapten-bearing liposomes.
The interaction of human peripheral blood lymphocytes with liposomes containing DNP-aminocaproyl-phosphatidylethanolamine together with either egg yolk or dipalmitoylphosphatidylcholine has been investigated. When lymphocytes were incubated with liposomes at 37 degrees C, the aqueous compartment (86Rb+) and the lipid portion (3H-lipid) of the liposomes became cell associated to an equivalent extent. At 0 degrees C, however, the incorporation of 3H-lipid exceeded that of 86Rb+. Lymphocyte-liposome interactions were accompanied by the transfer of DNP to the surface of the lymphoid cell as measured by susceptibility to complement in the presence of anti-DNP antibody. Hapten transfer was not limited to liposome interactions with lymphocytes, but occurred also with other cells (e.g., Chang cells). Hapten transfer could also be demonstrated by susceptibility to K cell-mediated lysis. These findings suggest that liposomes may prove to be a useful vehicle for the transfer of new antigenic determinants onto cell surfaces. The implications of these findings are discussed in the context of using liposomes as targets for cell-mediated cytotoxic attack.
D000818:Animals; D002462:Cell Membrane / Q000276:immunology; D002645:Chickens; D004136:Dinitrobenzenes / Q000378:metabolism; D000939:Epitopes; D006241:Haptens; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology; D008081:Liposomes / Q000378:metabolism; D008214:Lymphocytes / Q000276:immunology*; D008954:Models, Biological
D004136:Dinitrobenzenes; D000939:Epitopes; D006241:Haptens; D008081:Liposomes