title: Orseillin BB and crystal violet, a differential staining combination for paraffin sections and water mounts of fungi.
abstract: A saturated solution of orseillin BB in 3% acetic acid followed by a 1% aqueous solution of crystal violet provides an excellent differential staining for sections of ascomycetous fructifications. The technique stains a wide variety of fungus and host cells, revealing considerable morphological and cytological detail. It is appropriate for microscope slides both of unfixed material mounted in water and of picric acid-fixed paraffin sections.
mesh_terms: D000085:Acetates; D004396:Coloring Agents; D005658:Fungi / Q000166:cytology*; D005840:Gentian Violet; D010232:Paraffin; D013194:Staining and Labeling; D014867:Water
chemical_list: D000085:Acetates; D004396:Coloring Agents; D014867:Water; D010232:Paraffin; D005840:Gentian Violet