Detection of oncornavirus-like particles in normal and tumorigenic rat liver cell cultures.
Six epithelial cell cultures established from adult Wistar rat liver were examined by electron microscopy for oncornavirus-like particles. Two heteroploid and tumorigenic cultures demonstrated many cells with virus-like particles of type-A and type-C morphology. The remaining four, composed of diploid nontumorigenic cells, demonstrated similar virus-like particles but in few cells and only after bromodeoxyuridine treatment. The virus-like particles in the tumorigenic cultures were identified as a rat type-C virus by serological assays.
D000956:Antigens, Viral / Q000032:analysis; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002478:Cells, Cultured; D007181:Inclusion Bodies, Viral / Q000648:ultrastructure; D008099:Liver / Q000382:microbiology*; D008854:Microscopy, Electron; D009374:Neoplasms, Experimental / Q000382:microbiology*; D009858:Oncogenic Viruses / Q000302:isolation & purification*; D012328:RNA Viruses / Q000302:isolation & purification*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D012190:Retroviridae / Q000302:isolation & purification
D000956:Antigens, Viral; D012194:RNA-Directed DNA Polymerase; D001973:Bromodeoxyuridine