Human corneal endothelium in organ culture. The influence of temperature and medium of incubation.
Forty-four human corneas from patients between 21 and 86 years were incubated in Eagle's minimum essential medium with Earle's salts 10--46 h post mortem. The influence of incubation temperature and composition of the medium on endothelial survival was evaluated. Whole corneas were stained by alizarine red. Recent cell loss was indicated by morphological alterations in the endothelial pattern. After 20--28 h of incubation minimum cell loss was found at 31 degrees C when 8% Dextrane-250 and 20% serum or 8% Dextrane-500 and 10% serum was added to the medium.
D000328:Adult; D000367:Age Factors; D000368:Aged; D002470:Cell Survival / Q000187:drug effects; D003315:Cornea / Q000166:cytology* / Q000187:drug effects; D003470:Culture Media; D003911:Dextrans / Q000494:pharmacology; D004727:Endothelium / Q000166:cytology / Q000187:drug effects; D006801:Humans; D008875:Middle Aged; D009924:Organ Culture Techniques; D013194:Staining and Labeling; D013696:Temperature; D013997:Time Factors
D003470:Culture Media; D003911:Dextrans