Sodium pyrophosphate inhibition of RNA.DNA hybrid degradation by reverse transcriptase.
Sodium pyrophosphate inhibits synthesis of anticomplementary DNA during a reverse transcriptase (RNA-directed DNA nucleotidyltransferase, EC 2.7.7.7) catalyzed reaction. In the presence of pyrophosphate, the complementary DNA remains stably complexed to the RNA template. In the absence of pyrophosphate, the DNA. RNA hybrid template is degraded and anticomplementary DNA is synthesized. High concentrations of additives containing phosphodiester bonds appear to inhibit the ribonuclease H activity (hybrid nuclease, EC 3.1.4.34) of the reverse transcriptase, therby preventing formation of RNA primers necessary for the synthesis of anticomplementary DNA.
D011756:Diphosphates / Q000494:pharmacology*; D007700:Kinetics; D008836:Micrococcal Nuclease; D009693:Nucleic Acid Hybridization; D017955:Poliovirus; D012367:RNA, Viral; D018894:Reverse Transcriptase Inhibitors
D011756:Diphosphates; D012367:RNA, Viral; D018894:Reverse Transcriptase Inhibitors; D008836:Micrococcal Nuclease