The direct demonstration of histamine release in allergic reactions in the skin using a skin chamber technique.
We have adapted a skin chamber technique to permit sampling of fluid at the skin window sites of pollen antigen-induced allergic reactions. Low background levels of histamines are formed in control chambers, whereas significantly increased (p less than 0.01) amounts are found within 30 min following ragweed application in sensitized subjects.
D000941:Antigens / Q000008:administration & dosage; D006636:Histamine Release; D006801:Humans; D008722:Methods; D011058:Pollen; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology*; D012867:Skin / Q000276:immunology*
D000941:Antigens