Effect of Hoechst 33258 on Chinese hamster chromosomes.
Cells of the Chinese hamster strain C-125 were treated for different time intervals with H 33258, a bibenzimidazole derivative. The same compound was used to stain fixed cells of the same strain. H 33258 induced in cells in culture specific areas of reduced spiralization on the metaphase chromosomes of some cells. These probably correspond to DNA segments rich in A-T bases interspersed along the chromosomes. Probably H 33258 acts during S period of cell cycle. The banding obtained by staining with H 33258 is similar to that induced by quinacrine dihydrochloride but shows a better resolution.
D000227:Adenine Nucleotides / Q000032:analysis; D000818:Animals; D001562:Benzimidazoles; D006690:Bisbenzimidazole; D002875:Chromosomes / Q000032:analysis*; D006224:Cricetinae; D005456:Fluorescent Dyes; D008938:Mitosis; D011796:Quinacrine; D013194:Staining and Labeling; D013942:Thymine Nucleotides / Q000032:analysis
D000227:Adenine Nucleotides; D001562:Benzimidazoles; D005456:Fluorescent Dyes; D013942:Thymine Nucleotides; D011796:Quinacrine; D006690:Bisbenzimidazole