Improved nuclear staining with mordant blue 3 as a hematoxylin substitute.
For progressive staining 1 g mordant blue 3, 0.5 g iron a alum and 10 ml hydrochloric acid are combined to make 1 liter with distlled water. Paraffin sections are stained 5 minutes blued in 0.5% sodium acetate for 30 seconds and counterstained with eosin. For regressive staining, 1 g dye, 9 g iron alum and 50 ml acetic acid are combined to make 1 liter with distilled water. Staining time is 5 minutes followed by differentiation in 1% acid alcohol and blueing in 0.5% sodium acetate. Counterstain with eosin. In both cases results very closely results very resemble a good hematoxylin and eosin.
D001557:Benzenesulfonates; D002467:Cell Nucleus; D055598:Chemical Phenomena; D002621:Chemistry; D004396:Coloring Agents; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin; D006801:Humans; D013194:Staining and Labeling / Q000379:methods*; D014320:Triphenylmethyl Compounds
D001557:Benzenesulfonates; D004396:Coloring Agents; D014320:Triphenylmethyl Compounds; C010332:solochrome cyanine R; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin