Involvement of host Fc receptors in antibody-mediated cutaneous basophil hypersensitivity reactions.
Cutaneous basophil hypersensitivity (CBH) reactions are heterogeneous delayed time course basophil-rich responses that can be mediated by either T cells, B cells, or serum antibodies. The current study examined the mechanism by which antibodies mediate CBH in guinea pigs. Fc competition experiments were constructed by passively transferring mixtures of anti-KLH serum and normal heterologous gamma-globulins. It was found that rabbit IgG and its isolated and purified Fc fragment [but not the (Fab')2 fragment] inhibited the ability of guinea pig immune serum to transfer CBH. Concurrent inhibition of transferred KLH-specific CBH and systemic passive cutaneous anaphylaxis (PCA) reactions by rabbit IgG or its Fc fragment, and not by sheep or bovine gamma-globulins, indicated that Fc receptors on cutaneous mast cells were probably involved in both CBH and PCA. It was also found that the basophil aspect of delayed cutaneous responses elicited by PHA was inhibited by Fc competition maneuvers. This could mean that some forms of apparently T cell-mediated CBH may be T cell dependent, but via secretion of molecules that bind to Fc receptors, as seems required in antibody-mediated CBH.
D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000918:Antibody Specificity; D001491:Basophils / Q000276:immunology*; D005260:Female; D006168:Guinea Pigs; D006967:Hypersensitivity / Q000276:immunology*; D007116:Immunization, Passive; D007128:Immunoglobulin Fragments; D007074:Immunoglobulin G; D011817:Rabbits; D011961:Receptors, Fc / Q000276:immunology*; D012871:Skin Diseases / Q000276:immunology*; D005719:gamma-Globulins / Q000494:pharmacology
D000906:Antibodies; D007128:Immunoglobulin Fragments; D007074:Immunoglobulin G; D011961:Receptors, Fc; D005719:gamma-Globulins