title: Specific restoration of delayed hypersensitivity by lymphoid tissue extracts.
abstract: Mice lose demonstrable delayed hypersensitivity (DH) to DNFB, picryl chloride, or sheep red blood cells. Reconstitution of immune responsiveness can be accomplished by administration of cell-free lysates of spleens from mice with active DH to structurally related, but not to unrelated antigens. Peritoneal exudate cell lysates from mice with active DNFB-DH also restore DH to this antigen. Sera from sensitized mice, and sera and lymphoid tissue extracts from unsensitized mice are without activity. The restorative property of splenic lysates from DNFB-sensitized mice is unstable at 56 degrees C, not sedimented at 90,000 X G and inactivated by trypsin or magnesium ions. The presence of unexpressed, restorable DH may provide a biologic basis for the so called "transfer factor" phenomenon.
mesh_terms: D000818:Animals; D004139:Dinitrofluorobenzene / Q000276:immunology; D004431:Ear, External / Q000276:immunology / Q000473:pathology; D000939:Epitopes; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D008221:Lymphoid Tissue / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010853:Picryl Chloride / Q000276:immunology; D012756:Sheep; D012882:Skin Tests; D013154:Spleen / Q000276:immunology; D014020:Tissue Extracts / Q000494:pharmacology*
chemical_list: D000939:Epitopes; D014020:Tissue Extracts; D004139:Dinitrofluorobenzene; D010853:Picryl Chloride