[Porton uptake by isolated chloroplasts during cyclic and non-cyclic electron transport catalyzed by photosystem I in the presence of ferredoxin].
Proton uptake by isolated chloroplasts during cyclic electron transport in the presence of ferredoxin and under NADP+ reduction from the ascorbate--TMPD donor pair under anaerobic conditions was studied. It was found that during cyclic transport the proton uptake is less intensive than under NADP+ reduction. In the presence of ATP the proton uptake is increased in the first case and is decreased in the second one. During cyclic transport in the presence of gramicidin D the proton uptake is completely suppressed and under NADP+ reduction is decreased down to 0,08--0,09 mk equiv H+ per mg of chlorophyll, irrespective of ferredoxin or NADP+ concentrations. The role of ferredoxin NADP+ reductase in a proton uptake by thylakoids is discussed.
D000255:Adenosine Triphosphate / Q000378:metabolism; D001693:Biological Transport, Active / Q000187:drug effects; D002736:Chloroplasts / Q000378:metabolism*; D004579:Electron Transport; D005288:Ferredoxins / Q000378:metabolism*; D006096:Gramicidin / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D009249:NADP / Q000378:metabolism; D010788:Photosynthesis / Q000187:drug effects
D005288:Ferredoxins; D006096:Gramicidin; D009249:NADP; D000255:Adenosine Triphosphate
Pogloshchenie protonov izolirovannymi khloroplastami pri tsiklicheskom i netsiklicheskom transportakh elektronov, kataliziruemykh fotosistemoĭ I v prisutstvii ferredoksina.