Characterization and quantitation of murine-delayed hypersensitivity responses elicted with particle-associated human gamma-globulin.
Parameters delineating the utilization of particle-associated human gamma-globulin to quantitate murine-delayed hypersensitivity (DHS) responses include: (1) the conditions used to prepare HGG-coated polystyrene latex particles; (2) the marked increase in sensitivity of detection of delayed immune responsiveness using particle-bound HGG; (3) a histologic analysis of the lesions induced by HGG-latex in DHS mice, and (4) the increased retention of particle-associated as opposed to soluble HGG at the site of challenge. The potential utilization of particle-bound antigens as a sensitive method of assessing the role of delayed-type responsiveness in tumor and transplantation immunity is discussed.
D000818:Animals; D000917:Antibody Formation; D002417:Cattle; D003520:Cyclophosphamide / Q000494:pharmacology; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D005528:Foot / Q000276:immunology; D006801:Humans; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D007840:Latex / Q000494:pharmacology; D008297:Male; D051379:Mice; D008863:Microspheres; D012710:Serum Albumin, Bovine / Q000276:immunology; D012995:Solubility; D005719:gamma-Globulins / Q000494:pharmacology*
D007840:Latex; D005719:gamma-Globulins; D012710:Serum Albumin, Bovine; D003520:Cyclophosphamide