Interactions of rat alpha-foetoprotein with bilirubin.
The binding of bilirubin to rat-alpha-foetoprotein has been measured by changes in the absorption spectrum and by the appearance of two intense circular-dichroism bands. Furthermore binding of this bile pigment has been demonstrated by a decrease of its enzymic oxidation rate and by competition experiments with bovine serum albumin-agarose. The binding parameters have been determined as follows: n = 1.0 mol bound/mol of protein and Ka = 2.9 X 10(6) M-1. Competition of oestradiol for the bilirubin-binding site has not been established.
D000818:Animals; D001663:Bilirubin; D001665:Binding Sites; D001667:Binding, Competitive; D002942:Circular Dichroism; D004958:Estradiol; D011485:Protein Binding; D051381:Rats; D012685:Sepharose; D012710:Serum Albumin, Bovine; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry; D000509:alpha-Fetoproteins
D000509:alpha-Fetoproteins; D012710:Serum Albumin, Bovine; D004958:Estradiol; D012685:Sepharose; D001663:Bilirubin