Effect of polyamines on yeast cell-free protein synthesizing system. II. Increase stability of cell-free system in the presence of spermine.
The addition of spermine, at concentration which stimulates protein synthesis, to the yeast cell-free system significantly increases the thermal stability of the latter. Similar stabilizing effect of polyamine is observed for ribosome-poly U-ac-phe-tRNA complexes. These results suggest that the stimulatory effect of polyamines on the in vitro protein synthesis might be partly due to the increased stability of ribosomes and ribosome-peptydyl-tRNA complexes.
D002474:Cell-Free System; D004355:Drug Stability; D005656:Fungal Proteins / Q000096:biosynthesis*; D008274:Magnesium / Q000494:pharmacology; D010649:Phenylalanine / Q000031:analogs & derivatives / Q000378:metabolism; D012343:RNA, Transfer / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013096:Spermine / Q000494:pharmacology*; D013696:Temperature
D005656:Fungal Proteins; D013096:Spermine; D010649:Phenylalanine; D012343:RNA, Transfer; D008274:Magnesium