Physicochemical analysis of the deoxyribonucleic acid product of murine intracisternal A particle RNA-directed DNA polymerase.
The nascent DNA transcript of intracisternal A particle RNA-directed DNA polymerase appeared to be covalently linked to an RNA primer. Fidelity of transcription is evident since the DNA transcript hybridized specifically back to 35-70 S RNA of intracisternal A particles but not with heterologous RNAs. This DNA transcript has an approximate molecular weight of 145 000, estimating 350 polynucleotides and base ratios with an excess of dGMP.
D001665:Binding Sites; D002460:Cell Line; D004273:DNA, Neoplasm / Q000096:biosynthesis; D046911:Macromolecular Substances; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*; D014158:Transcription, Genetic
D004273:DNA, Neoplasm; D046911:Macromolecular Substances; D012194:RNA-Directed DNA Polymerase