[Cytospectrophotometric analysis of various color reactions for acid and basis proteins in nerve tissue cells].
Polyvinyl formal films soaked with various concentrations of proteins as well as Carnoy-fixed paraffin sections of rat brain were stained with various colour reactions for acidic and basic proteins. Sufficient specificity, reproductivity, sensitivity and applicability for cytospectrophotometric determinations have been shown for the staining of acidic proteins with Toluidine blue 0 (pH 9.0) or with Fast green FCF (pH 2.6), and for the staining of basic proteins with Alcian blue (pH 10.0), or with Fast green FCF (pH 8.2). Importance of cytophotometric analysis of individual protein fractions is outlined for the functional cytochemistry of the nervous system.
D000418:Albumins / Q000032:analysis*; D000423:Alcian Blue; D000818:Animals; D001921:Brain / Q000166:cytology*; D002467:Cell Nucleus / Q000032:analysis; D006651:Histocytochemistry; D006657:Histones / Q000032:analysis*; D009419:Nerve Tissue Proteins / Q000032:analysis*; D009474:Neurons / Q000032:analysis; D051381:Rats; D013053:Spectrophotometry / Q000379:methods; D013194:Staining and Labeling; D014048:Tolonium Chloride
D000418:Albumins; D006657:Histones; D009419:Nerve Tissue Proteins; D014048:Tolonium Chloride; D000423:Alcian Blue
Tsitospektrofotometricheskiĭ analiz nekotorykh tsvetnykh reaktsiĭ na kislye i osnovnye belki v kletkakh nervnoĭ tkani