title: [Immunochemical approach to explaining the molecular organization and mechanism of action of Na+,K+-ATPase].
abstract: Application of immunochemical methods for elucidating the Na+, K+-ATPase molecular organization and mechanism of action is discussed. The data of some authors who have studied the purified preparations of Na+, K+-ATPase from different sources show that both the catalytic subunit of the enzymic complex and its sialoglycoproteid have antigenic determinants. Antibodies, binding with these determinants, may either change or produce no effect on the enzymic process, link with the active transport of Na+ and K+. Antibodies used as conformation-sensitive explorers may yield the interesting information on organization of the Na+, K+-ATPase complex in the membrane and its conformation in different functional states which simultaneously with the data of the kinetic analysis and reconstruction of this enzymic complex would contribute to deciphering the mechanism of the Na+ and K+ active transport through biomembranes.
mesh_terms: D000818:Animals; D001921:Brain / Q000201:enzymology; D055598:Chemical Phenomena; D002621:Chemistry; D004285:Dogs; D000939:Epitopes; D004910:Erythrocyte Membrane / Q000201:enzymology; D006801:Humans; D007668:Kidney / Q000201:enzymology; D008968:Molecular Conformation; D010042:Ouabain; D011817:Rabbits / Q000276:immunology; D051381:Rats; D012756:Sheep / Q000276:immunology; D000254:Sodium-Potassium-Exchanging ATPase / Q000276:immunology; D013045:Species Specificity
chemical_list: D000939:Epitopes; D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase
vernacular_title: Immunokhimicheskiĭ podkhod k vyiasneniiu molekuliarnoĭ organizatsii i mekhanizma deĭstviia Na+,K+-ATP-azy.