Cytochemical identification of T and B cells in situ in mouse lymphoid tissue and lymph nodes from the rat, gerbil and cat.
The use of enzymes as markers of T or B cells in tissue sections has been studied in mouse lymphoid tissue and lymph nodes from the gerbil, rat and cat. Lymphocytes in the T-cell areas of murine lymph nodes and spleen contained discrete dots of non-specific esterase and N-acetyl-beta-D-glucosaminidase (beta-glucosaminidase) activity, with weak acid phosphatase activity. Lymphocytes in the B-cell areas lacked this discrete staining. Cortical thymocytes contained slight esterase activity while medullary thymocytes were strongly positive for both esterase and beta-glucosaminidase. Lymphocytes with a T-cell staining pattern were only occasionally seen in lymph nodes from Nude (nu/nu) mice. ATPase staining was restricted to lymphocytes in the B-cell areas; weak 5'-nucleotidase staining was only present in a frew lymphocytes in both T- and B-cell areas. Blast cells stimulated by in vivo injection of ConA or PHA in the mouse showed strong discrete enzyme activity for non-specific esterase and beta-glucosaminidase. Lipopolysaccharide-stimulated blast cells and cells within germinal centres lacked this discrete staining. Comparison of lymph nodes from the gerbil, rat and cat suggested at least on enzymes as a T-cell marker in each species although considerable variation in staining profiles was seen in the different species.
D000135:Acid Phosphatase / Q000032:analysis; D000818:Animals; D001402:B-Lymphocytes / Q000201:enzymology*; D002415:Cats; D004950:Esterases / Q000032:analysis; D005849:Gerbillinae; D005966:Glucuronidase / Q000032:analysis; D006596:Hexosaminidases / Q000032:analysis; D008198:Lymph Nodes / Q000166:cytology / Q000201:enzymology; D008221:Lymphoid Tissue / Q000166:cytology / Q000201:enzymology; D008247:Lysosomes / Q000201:enzymology; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D051381:Rats; D013045:Species Specificity; D013194:Staining and Labeling; D013601:T-Lymphocytes / Q000201:enzymology*
D004950:Esterases; D000135:Acid Phosphatase; D006596:Hexosaminidases; D005966:Glucuronidase