[Reverse transcription of the RNA of eukaryotes and viruses. Conditions for obtaining a long product].
The receiving of full-size DNA-copies of RNAs is necessary for molecular hybridisation experiments as well as for synthesis of recombinant bacterial plasmids with eucariotic DNA sequences. Some authors received such cDNAs for different RNAs with a help of variations in reaction conditions. In this article it is shown that such empirically chosen conditions mainly had an influence on a secondary structure of RNA-templates and that only in such a case the synthesis of the DNA-product with sizes corresponding to the RNA-template is possible.
D009189:Avian Myeloblastosis Virus / Q000201:enzymology; D004247:DNA / Q000096:biosynthesis; D004277:DNA, Single-Stranded / Q000096:biosynthesis; D003902:Detergents; D008274:Magnesium; D008968:Molecular Conformation; D008970:Molecular Weight; D009994:Osmolar Concentration; D012333:RNA, Messenger; D012194:RNA-Directed DNA Polymerase; D013696:Temperature; D013816:Thermodynamics; D014157:Transcription Factors; D014158:Transcription, Genetic
D004277:DNA, Single-Stranded; D003902:Detergents; D012333:RNA, Messenger; D014157:Transcription Factors; D004247:DNA; D012194:RNA-Directed DNA Polymerase; D008274:Magnesium
Obratnaia transkriptsiia RNK éukariot i virusov. Usloviia polucheniia dlinnogo produkta.