New staining method for the detection of sister-chromatid exchanges in BrdU-labelled chromosomes.
A direct Giemsa technique is described for the selective staining of Brd-U-substituted chromatin. It can advantageously be used for sister-chromatid exchange analysis and for the detailed cytological study of the mouse kinetochores. Presumptive inversions within the centromeric region of chromosomes 4 and 18 in the C57Black/6J mouse are described.
D000818:Animals; D001973:Bromodeoxyuridine; D002503:Centromere / Q000648:ultrastructure; D002842:Chromatids / Q000648:ultrastructure*; D002843:Chromatin / Q000648:ultrastructure*; D002999:Clone Cells; D008677:Metaphase; D051379:Mice; D008810:Mice, Inbred C57BL; D013194:Staining and Labeling / Q000379:methods
D002843:Chromatin; D001973:Bromodeoxyuridine