Studies on human hyperplastic prostates maintained in organ culture.
Prostates from patients with prostatic hyperplasia were maintained in organ culture for periods up to 8 days. Explants were disaggregated and studied after 2, 4, and 8 days of culture, as well as before culture. Although the data varied from patient to patient, short periods of culture (2 to 4 days) resulted in increased numbers of cells with histochemically demonstrable acid phosphatase; however, by 8 days of culture, cells with histochemically demonstrable acid phosphatase decreased in number. The ratio of acid phosphatase to beta-glucuronidase expressed per cell or per milligram of protein decrease; with time in culture.
D000135:Acid Phosphatase / Q000378:metabolism; D004847:Epithelial Cells; D004848:Epithelium / Q000201:enzymology; D005966:Glucuronidase / Q000378:metabolism; D006801:Humans; D008297:Male; D009924:Organ Culture Techniques; D011467:Prostate / Q000201:enzymology*; D011470:Prostatic Hyperplasia / Q000201:enzymology; D013997:Time Factors
D000135:Acid Phosphatase; D005966:Glucuronidase