The application of photobleaching to sequential staining of antigen-antibody precipitates in gels for prostatic acid phosphatase and protein.
A modified procedure is described for the sequential staining of agar precipitates of seminal plasma and antiseminal plasma serum for acid phosphatase and total protein. The procedure employs Gomori's glycerophosphate-lead sulphide method for acid phosphatase, ultraviolet light for photobleaching and amidoschwartz for total protein staining. In a single agar diffusion plate, a minimum of 7 protein bands was observed, 3 of which contained acid phosphatase activity.
D000135:Acid Phosphatase / Q000032:analysis* / Q000528:radiation effects; D006801:Humans; D005779:Immunodiffusion; D008297:Male; D008722:Methods; D011467:Prostate / Q000032:analysis* / Q000201:enzymology; D011506:Proteins / Q000032:analysis* / Q000528:radiation effects; D012661:Semen / Q000032:analysis / Q000201:enzymology; D013194:Staining and Labeling; D014466:Ultraviolet Rays
D011506:Proteins; D000135:Acid Phosphatase