Cell types required for H-2-restricted cytotoxic responses generated by trinitrobenzene sulfonate-modified syngeneic cells or trinitrophenyl-conjugated proteins.
Murine spleen cells were fractionated over nylon wool or Sephadex G-10 columns, and the cell types involved in the generation of trinitrophenyl (TNP)-specific, H-2 restricted (TNP-self) cytotoxic effector cells were studied from cultures stimulated with trinitrobenzene sulfonate (TNBS)-modified syngeneic cells, TNP-conjugated soluble proteins such as bovine gamma-globulin (TNP-BGG), or bovine serum albumin (TNP-BSA). Unfractionated or nylon nonadherent responding cells generated such effectors, irrespective of whether the cultures were stimulated with TNBS-modified cells or TNP-conjugated proteins. TNP-modified T lymphocytes, B lymphocytes, and phagocyte-enriched spleen cells were all capable of stimulating TNP-self effectors. TNP-self effectors. TNP-self as well as allogeneic cytotoxic responses were dependent on the presence of a radioresistant non-T cell that was removed by Sephadex G-10 fractionation and was replaced by irradiated, Thy 1.2-negative, glass adherent spleen cells, enriched in phagocytic cells. Results obtained by using glass adherent cells that were allogeneic or semi-syngeneic to the responding cells indicated that H-2 homology was not required for efficient glass adherent cell function, and that the H-2 restriction of TNP-self effectors is not determined by these glass adherent cells.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D002417:Cattle; D002448:Cell Adhesion; D002469:Cell Separation; D002478:Cells, Cultured; D002850:Chromatography, Gel; D003602:Cytotoxicity, Immunologic; D006183:H-2 Antigens / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009578:Nitrobenzenes / Q000276:immunology*; D010586:Phagocytes / Q000276:immunology; D012710:Serum Albumin, Bovine / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D014301:Trinitrobenzenes / Q000276:immunology*; D005719:gamma-Globulins / Q000276:immunology*
D006183:H-2 Antigens; D009578:Nitrobenzenes; D014301:Trinitrobenzenes; D005719:gamma-Globulins; D012710:Serum Albumin, Bovine