[Conduct of Bacillus subtilis transformation in mice under conditions of immunologic suppression of exogenous DNAase 1 activity].
Bacillus subtilis transformation was conducted in the abdominal cavity of mice. The frequency of transformation was considerable decreased when bovine DNA-ase 1 (3-- 5microgram) was injected intraperitoneally to these animals. Immune rabbit gamma-globulins containing antibodies to bovine DNA-ase 1 inhibited in vivo the activity of DNA-ase 1, protected transforming DNA from the hydrolyzing effect of this enzyme. This model can be utilized in search for ways to preserve DNA injected into the animal organism for the purpose of genetical engineering.
D000818:Animals; D001412:Bacillus subtilis / Q000502:physiology*; D003851:Deoxyribonucleases / Q000494:pharmacology*; D005818:Genetic Engineering; D008722:Methods; D051379:Mice; D008954:Models, Biological; D010529:Peritoneal Cavity / Q000382:microbiology; D014170:Transformation, Genetic; D005719:gamma-Globulins / Q000494:pharmacology*
D005719:gamma-Globulins; D003851:Deoxyribonucleases
Provedenie transformatsii Bacillus subtilis v organizme mysheĭ v usloviiakh immunologicheskogo podavleniia aktivnosti ékzogennoĭ DNK-azy 1.