[Preparative isolation of alpha 2-macroglobulin, transferrin, albumin and study of their nonspecific gamma-inhibitory activity].
Profiles of distribution of non-specific gamma-inhibitors of influenza A2/Victoria/35/72 in donkey and horse sera were established by gel chromatography in Sephadex G-200. High and low molecular inhibitors were found in 19S and 4S serum fractions. Highly purified preparations of a2-macroglobulin, transferrine and albumin were isolated by a combination of methods of salt precipitation, gel chromatography on Sephadex G-100, G-200 and ion exchange on DEAE-Sephadex A-50. Heating sera resulted in a considerable increase of the antiviral activity of a2-macroglobulin and transferrine and a reduction of albumin activity.
D000818:Animals; D000998:Antiviral Agents; D006736:Horses / Q000276:immunology; D006358:Hot Temperature; D007106:Immune Sera / Q000032:analysis; D009980:Influenza A virus / Q000276:immunology*; D008722:Methods; D010527:Perissodactyla / Q000276:immunology; D012709:Serum Albumin / Q000302:isolation & purification; D014168:Transferrin / Q000302:isolation & purification; D000511:alpha-Macroglobulins / Q000302:isolation & purification
D000998:Antiviral Agents; D007106:Immune Sera; D012709:Serum Albumin; D014168:Transferrin; D000511:alpha-Macroglobulins
Preparativnoe vydelenie alpha 2-makroglobulina, transferrina, al'bumina i izuchenie ikh nespetsificheskoĭ gamma-ingibitornoĭ aktinosti