Isolation of chicken hemoglobin mRNA and synthesis of complementary DNA.
Chicken globin mRNA has been purified and partially characterized. Globin-specific sequences are found primarily as 9 S RNA, but also are found with ribosomal RNA, preferentially the 28 S moiety. The chicken globin mRNA preparation has been translated in the wheat germ and Krebs ascites cell-free systems. The products have been identified by sodium dodecyl sulfate-gel electrophoresis as the alpha- and beta-globin polypeptides. The globin mRNA is resolved into two asymmetric peaks by polyacrylamide gel electrophoresis in 98% formamide. The minor rapidly migrating peak consists primarily of alpha message while the major slowly migrating peak contains a mixture of alpha and beta messages. The synthesis of cDNA has been optimized and the products analyzed by polyacrylamide gel electrophoresis in 98% formamide. The products consist primarily of full copy transcripts that can be resolved into three discrete species.
D000818:Animals; D001483:Base Sequence; D002287:Carcinoma, Krebs 2 / Q000378:metabolism; D002645:Chickens; D002846:Chromatography, Affinity; D004247:DNA / Q000097:blood*; D004261:DNA Replication; D005260:Female; D005914:Globins / Q000096:biosynthesis; D006454:Hemoglobins / Q000096:biosynthesis*; D007700:Kinetics; D051379:Mice; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000097:blood / Q000302:isolation & purification; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism
D006454:Hemoglobins; D012333:RNA, Messenger; D005914:Globins; D004247:DNA; D012194:RNA-Directed DNA Polymerase