Characterization of human beta2-microglobulin by isoelectric focusing.
b2-Microglobulin (B2M) isolated from the urine of normal subjects and patients with cadaveric renal transplantation, showed 2 homologues by isoelectric focusing, one with a pI 5.3, the other with a pI 5.7. These proteins show identical molecular weights by dextran gel filtration and sodium dodecyl sulfate acrylamide gel electrophoresis. The immunogenic reactivity demonstrates partial identity using antiserum to human B2M from 2 different sources.
D001609:Beta-Globulins / Q000032:analysis*; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007525:Isoelectric Focusing; D016030:Kidney Transplantation; D008722:Methods; D014184:Transplantation, Homologous; D001613:beta 2-Microglobulin / Q000032:analysis* / Q000652:urine
D001609:Beta-Globulins; D001613:beta 2-Microglobulin