Nuclear DNA-content of parathyroid cells in adenomas, hyperplastic and normal glands.
Nuclear DNA-content in parathyroid cell imprints from adenomas, hyperplastic and normal glands was determined. Two principally different staining procedures were applied: paratoseaniline-Feulgen and ethidium-bromide staining. All nuclei, particularly with the ethidium-bromide method were registered as containing even multiples of the normal human diploid DNA-content. Normal and hyperplastic glands showed greater than or equal to 98 per cent diploid nuclei, the remaining fraction was tetraploid. In adenomas the frequency of diploid nuclei varied from 50-98 per cent; the remaining were predominantly tetraploid, but octaploid and higher even ploidity also occurred. A good correlation was found between nuclear DNA-content and nuclear diameter. The findings indicate that determination of nuclear DNA-content of parathyroid cells may be a valuable contribution in the differentiation between hyperplasia and adenoma.
D000236:Adenoma / Q000032:analysis*; D002467:Cell Nucleus / Q000032:analysis; D004247:DNA / Q000032:analysis*; D004273:DNA, Neoplasm / Q000032:analysis*; D006801:Humans; D006965:Hyperplasia; D010280:Parathyroid Glands / Q000166:cytology / Q000473:pathology*; D010282:Parathyroid Neoplasms / Q000473:pathology*; D011123:Polyploidy; D013194:Staining and Labeling
D004273:DNA, Neoplasm; D004247:DNA