Cobalt staining of Actinia equina nematocysts.
Nematocysts of Actinia equina are stained black by incubation in 2% CoCl2 followed by an aqueous wash and H2S treatment. They are also stained positively by morin. Nematocysts isolated from the acrorhage were found to have a high concentration of calcium of which only 30% was "free." It is suggested that the high concentration of calcium in the nematocysts accounts for their staining by cobalt and morin. Cobalt staining offers a simple and effective technique for investigation of nemotocysts.
D000818:Animals; D003063:Cnidaria / Q000033:anatomy & histology*; D003035:Cobalt; D004492:Edetic Acid / Q000494:pharmacology; D006862:Hydrogen Sulfide; D012615:Sea Anemones / Q000033:anatomy & histology*; D013194:Staining and Labeling
D003035:Cobalt; D004492:Edetic Acid; D006862:Hydrogen Sulfide