Lysolecithin analogs as adjuvants in delayed-type hypersensitivity in mice. I. Characterization of the adjuvant effect.
The adjuvant activity of 5 different lysolecithin analogs (LLA) has been studied in delayed-type hypersensitivity (DTH) using fowl gamma globulin, bovine serum albumin and the terpolymer L-glutamic acid 60-L-alanine30-L-tyrosine10(GAT), as antigens. Increased DTH responses, by factors of 1.8--2.0 in CBA and BALB/c mice, showed that LLA are immunopotentiators if given intraperitoneally (i.p.) or subcutaneously (s.c.) together with the antigen. The concentration range, within which LLA are active, is limited to 10--20 micrograms/mouse s.c. and 100--300 micrograms/mouse i.p. Adjuvanticity was tested as a function of the LLA structure. The most pronounced immunopotentiation was obtained with racemic 1-octadecyl-2-methylglycero-3-phosphorylcholine (ET18-O-CH3). The LLA became less active with decreasing numbers of C atoms in the alkyl chain.
D000276:Adjuvants, Immunologic; D000818:Animals; D000941:Antigens / Q000008:administration & dosage; D005260:Female; D006968:Hypersensitivity, Delayed; D008244:Lysophosphatidylcholines / Q000494:pharmacology*; D051379:Mice; D008815:Mice, Inbred Strains; D010455:Peptides / Q000008:administration & dosage / Q000276:immunology; D011108:Polymers; D012710:Serum Albumin, Bovine / Q000008:administration & dosage; D013329:Structure-Activity Relationship; D005719:gamma-Globulins / Q000008:administration & dosage
D000276:Adjuvants, Immunologic; D000941:Antigens; D008244:Lysophosphatidylcholines; D010455:Peptides; D011108:Polymers; D005719:gamma-Globulins; D012710:Serum Albumin, Bovine; C020749:GAT