[Staining the Nissl bodies in 4--10 microns thick Araldite sections with an area of about 2x2 cm (author's transl)].
For light microscopy 4--10 microns thick sections with an area of 2x2 cm o Araldite-embedded tissue (human autopsy brain) are stained in 1% aqueous methylene blue at 65 degrees C for 4--12 hours and are differentiated in 98% ethanol for some seconds or very few minutes. The Nissl bodies, nuclei and nucleoli are stained dark blue, the neuropil is nearly colourless.
D002540:Cerebral Cortex / Q000648:ultrastructure*; D005404:Fixatives; D006652:Histological Techniques; D006801:Humans; D008751:Methylene Blue; D009562:Nissl Bodies / Q000648:ultrastructure*; D012117:Resins, Synthetic; D013194:Staining and Labeling
D005404:Fixatives; D012117:Resins, Synthetic; D008751:Methylene Blue
Färbung der Nissl-Substanz in 4--10 micron dicken und 2x2 cm grossen Aralditschnitten