Immune response to phosphorylcholine. I. Characterization of the epitope-specific antibody.
The antibodies to phosphorylcholine induced in BALB/c mice were isolated and studied with a variety of biochemical and immunochemical methods. Analysis of the idiotype and the hapten-binding specificities showed no differences to the phosphorylcholine-binding myeloma protein TEPC 15, indicating a high degree of homogeneity. However, disc electrophoresis, isoelectric focusing and amino acid composition data indicated large differences in the structure of anti-phosphorylcholine antibody and TEPC 15. By these criteria the anti-phosphorylcholine antibodies from BALB/c mice are of oligoclonal origin.
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D002794:Choline / Q000031:analogs & derivatives*; D002999:Clone Cells / Q000276:immunology*; D003429:Cross Reactions; D000939:Epitopes; D005260:Female; D006241:Haptens; D007147:Immunoglobulin Light Chains / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis*; D007148:Immunoglobulin mu-Chains / Q000032:analysis; D007525:Isoelectric Focusing; D051379:Mice; D008807:Mice, Inbred BALB C; D009194:Myeloma Proteins / Q000032:analysis / Q000276:immunology; D010767:Phosphorylcholine / Q000276:immunology*
D000906:Antibodies; D000939:Epitopes; D006241:Haptens; D007147:Immunoglobulin Light Chains; D007075:Immunoglobulin M; D007148:Immunoglobulin mu-Chains; D009194:Myeloma Proteins; D010767:Phosphorylcholine; D002794:Choline