Biochemical evidence for viral-like characteristics in cerebrospinal fluids of brain tumor patients.
Previous work done in this laboratory has shown that human Central Nervous System tumors possess attributes in common with those found in RNA tumor viruses. Because of the intimate physical relationship between the circulating cerebro-spinal fluid and the Central Nervous System, particles derived from tumors in this system may very well be shedded into the surrounding fluid system thus displaying similar biochemical attributes. Twenty samples of cerebro-spinal fluids were examined. Fifteen were from patients with various types of tumors in various location of the Central Nervous System. Five were from patients with unrelated diseases. Eleven or 74% of those from tumor patients were found to be positive when simultaneous detection assays were done on their core structures. None of the control was positive. The [3H]DNA synthesized from core structures hybridized readily to polysomal RNAs from a solid tumor. Particles from them were found to have a density of 1.867 g/ml sucrose gradient.
D001932:Brain Neoplasms / Q000134:cerebrospinal fluid / Q000209:etiology / Q000382:microbiology*; D002499:Centrifugation, Density Gradient; D002555:Cerebrospinal Fluid / Q000382:microbiology*; D004279:DNA, Viral / Q000096:biosynthesis; D005260:Female; D006801:Humans; D008297:Male; D009693:Nucleic Acid Hybridization; D009858:Oncogenic Viruses / Q000302:isolation & purification*; D012328:RNA Viruses / Q000302:isolation & purification*; D012335:RNA, Ribosomal / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism
D004279:DNA, Viral; D012335:RNA, Ribosomal; D012367:RNA, Viral; D012194:RNA-Directed DNA Polymerase