Cytofluorometry of electromagnetically controlled cell dedifferentiation.
Cellular morphology changes, which appear related to dedifferentiation (despecialization), have been produced in vitro in the nucleated red blood cell of the frog. This has been achieved by controlled alterations in the electrochemical environment of these living cells, both by a selective modification of the ionic concentrations of an isotonic amphibian Ringer solution, and by the electromagnetic induction of pulsating current having specific waveform parameters. Laser flow microfluorometry shows that the modified Ringer solution is able, per se, to partially trigger the process in the same time interval that certain induced current waveforms can significantly affect the number of cells in the so-called dedifferentiated state. It has also been found that, for a given waveform, the repetition rate appears to have a significant effect on the rate of cell change. Preliminary automated image analysis of cell smears suggests that dedifferentiated and normal cells have the same integrated optical density but different nuclear areas. In conclusion, it appears that, after the initial electrochemical trigger, the early stage of the process, when the cells move from a state of specialized function to one of less specific activity, is the unfolding of their chromatin supercoil, not involving DNA synthesis. Then cytofluorometry allowed us to identify, for the first time, fundamental modifications which occur in the cell nucleus under electromagnetic exposure.
D000165:Acridine Orange; D000818:Animals; D001001:Anura; D002454:Cell Differentiation; D002467:Cell Nucleus / Q000032:analysis; D003584:Cytological Techniques; D004247:DNA / Q000032:analysis; D004574:Electromagnetic Fields; D004912:Erythrocytes / Q000166:cytology*; D005260:Female; D005470:Fluorometry; D011894:Rana pipiens; D013194:Staining and Labeling
D004247:DNA; D000165:Acridine Orange