Analysis of some histochemical colour reactions for cytospectrophotometry of basic and acidic proteins in nervous tissue cells.
Cytospectrophotometric analysis of model systems and of single cells in Carnoy-fixed paraffin sections of the nervous tissue has shown two histochemical colour reactions for basic proteins and two reactions for acidic proteins to obey Bouger-Lambert-Beer's law. Basic proteins can be stoichiometrically stained either with Fast Green FCF, pH 8.2, or with heparin-Alcian Blue; acidic proteins either with Fast Green, FCF, pH 2.6, or with Toluidine Blue o. basing on better reproducibility of cytochemical results and sufficiently good preservation of cell structure, heparine-Alcian Blue is recommended for cytospectrophotometric determinations of basic proteins. To stain acidic proteins both Fast Green, pH 2.6, and Toluidine Blue O are recommended, each of the dyes being characterized by its own advantages and disadvantages.
D000818:Animals; D001923:Brain Chemistry; D006651:Histocytochemistry; D009419:Nerve Tissue Proteins / Q000032:analysis*; D051381:Rats; D013053:Spectrophotometry; D013194:Staining and Labeling
D009419:Nerve Tissue Proteins