title: Immunohistochemistry on Bouin-fixed fetal tissue, stored for thirty years in ethanol.
abstract: Human fetal tissue fixed with Bouin and kept for 30 years in ethanol is still useful in immunohistochemistry. The localization of beta-endorphin immunoreactive cells in the pituitary is given as example.
mesh_terms: D004723:Endorphins / Q000032:analysis*; D000431:Ethanol; D005260:Female; D005333:Fetus / Q000166:cytology*; D006651:Histocytochemistry; D006801:Humans; D007202:Indicators and Reagents; D010903:Pituitary Gland, Anterior / Q000032:analysis* / Q000166:cytology; D011247:Pregnancy; D013194:Staining and Labeling; D013997:Time Factors
chemical_list: D004723:Endorphins; D007202:Indicators and Reagents; D000431:Ethanol