Blocking of HLA and B lymphocyte alloantigens with F (ab')2 fragments of rabbit antibodies.
F(ab')2 fragments of rabbit antibodies specific for human B lymphocytes will block human anti-B lymphocyte alloantibodies. F(ab')2 of anti-beta2m will block HLA alloantibodies. The F(ab')2 fragments can be used to improve the typing of B cell lines and certain leukemia cells which express both HLA and B lymphocyte alloantigens. Pretreatment of these cells with anti-beta2m F(ab')2 preparations allows the observation of B cell alloantigen reactions independently of HLA reactions. Similar use of rabbit anti-B cell F (ab')2 allows HLA reactions to be observed free from the "extra reactions" caused by B alloantibodies that are present in many HLA typing sera.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D001609:Beta-Globulins / Q000276:immunology*; D001667:Binding, Competitive; D006680:HLA Antigens / Q000032:analysis*; D006649:Histocompatibility Antigens / Q000032:analysis*; D007140:Immunoglobulin Fab Fragments; D011817:Rabbits; D001613:beta 2-Microglobulin / Q000276:immunology*
D001609:Beta-Globulins; D006680:HLA Antigens; D006649:Histocompatibility Antigens; D007140:Immunoglobulin Fab Fragments; D001613:beta 2-Microglobulin