Radioimmunoassay of "pregnancy-specific"-beta 1-glycoprotein (SP1).
A radioimmunoassay for SP1 ("pregnancy-specific" beta 1-glycoprotein) is described in which a sensitivity of 0.1 microgram/L was achieved by use of an antiserum with Ka approximately equal to 1.7 x 10(11) L/mol and delayed addition of a 125I-SP1 tracer (specific acty, 25 Ci/g) that was stable for use in the assay for at least six to seven weeks. We also report an additional anti-SP1 serum, from an early bleeding, that displayed "apparent positive cooperativity" in the IgG but not the IgM fraction.
D001609:Beta-Globulins / Q000032:analysis*; D005260:Female; D006023:Glycoproteins / Q000097:blood*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D008832:Microchemistry; D011247:Pregnancy; D011863:Radioimmunoassay / Q000379:methods
D001609:Beta-Globulins; D006023:Glycoproteins; D007074:Immunoglobulin G; D007075:Immunoglobulin M