Purification of measles virus with preservation of infectivity and antigenicity.
Measles virus Edmonston strain was purified by ultrafiltration followed by two successive sedimentations through sucrose. Purified virus retained infectivity and, when used as an immunogen, elicited high titred antibody to measles antigens by conventional serology. The measles preparations were examined by SDS-PAGE followed by staining. In addition, following PAGE, the purity of these preparations was assessed immunochemically using antisera directed to measles and host cell antigens. The results of these studies demonstrate the utility of the purification method for the preparation of milligram quantities of relatively pure measles virus.
D000939:Epitopes; D008459:Measles virus / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D010455:Peptides / Q000032:analysis / Q000276:immunology; D014462:Ultrafiltration; D014764:Viral Proteins / Q000032:analysis / Q000276:immunology
D000939:Epitopes; D010455:Peptides; D014764:Viral Proteins