New approach to evaluation of proteinuric states.
We evaluated the use of immunonephelometric methods for measuring specific urinary proteins. Using a nephelometer to detect light scattering (angle, 31 degrees), we measured some proteins immunonephelometrically in serum and aliquots of 24-h urines from 50 apparently healthy children, ages 2-17 years. The mean urinary excretion rate (mg/24h) and the range of values was: for albumin 5.5 (range, 0-13.3), for transferrin 0.5 (0-1.9, for IgG 3.3 0-12), and for alpha 2-macroglobulin 0.6 (0-2.3). Direct comparison of the values for pathological urines with those for a reference population may offer more meaningful information concerning the integrity of the glomerular basement membrane than is provided by protein selectivity indices, and measuring a plasma protein such as albumin in urine may better define pathological proteinuria.
D000293:Adolescent; D000419:Albuminuria / Q000175:diagnosis; D002648:Child; D002675:Child, Preschool; D005069:Evaluation Studies as Topic; D006801:Humans; D007158:Immunologic Techniques; D009404:Nephrotic Syndrome / Q000652:urine; D011507:Proteinuria / Q000601:surgery*; D012542:Scattering, Radiation; D014168:Transferrin / Q000652:urine; D000511:alpha-Macroglobulins / Q000652:urine
D014168:Transferrin; D000511:alpha-Macroglobulins