Enhancement of the specific cytotoxic reactivity of HLA antigens on lymphocytes stored at +4 degrees C for 24 hours.
The lymphocytes of some patients (especially with malignancies) as well of healthy subjects, which had been isolated on the day of blood withdrawal from the heparinized blood, show negative or slightly positive reaction with some corresponding HLA sera; after 24 h of storing in the refrigerator at +4 degrees C the lymphocytes tend to restore the cytotoxic reactivity of HLA antigens, and thus, a strongly positive reaction can be recorded. The cause of this phenomenon may be ascribed to the anticomplementary effect of the lymphocyte suspension in barbital buffer.
D001793:Blood Preservation; D003165:Complement System Proteins; D003602:Cytotoxicity, Immunologic; D006680:HLA Antigens; D006801:Humans; D008214:Lymphocytes / Q000276:immunology*; D013696:Temperature
D006680:HLA Antigens; D003165:Complement System Proteins