The use of trypan blue for counterstaining in the Sepharose bead immunofluorescence test. The application of the test for the demonstration of primate retrovirus-specific antibodies and antigens.
The Sepharose bead immunoflurorescence test was performed by counterstaining the beads with trypan blue. This results in a red staining of negative beads which allows an easy distinction from positive green-fluorescent beads. Sepharose beads conjugated with viral proteins or antiviral antibodies were used to demonstrate Mason-Pfizer monkey virus (MPMW)- and simian sarcoma virus (SSV) - specific antigens or antibodies. The test shows a high sensitivity and specificity and needs a small amount of material.
D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D012190:Retroviridae / Q000276:immunology*; D012842:Sarcoma Virus, Woolly Monkey / Q000276:immunology; D012685:Sepharose; D013194:Staining and Labeling; D014343:Trypan Blue
D000914:Antibodies, Viral; D000956:Antigens, Viral; D012685:Sepharose; D014343:Trypan Blue