T-cell populations specifically depleted of alloreactive potential cannot be induced to lyse H-2-different virus-infected target cells.
Mouse lymphocyte populations of one parental H-2 type (A) were specificially depleted of alloreactive potential by filtration through irradiated A X B F1 recipients, and thoracic duct cells were then stimulated with virus in an A X B F1 environment. Experiments using T cells that had previously been exposed to influenza virus in the context of A established that cross-priming for recognition of viral components expressed on H-2-different (B) target cells does not occur. Furthermore, immunologically naive T cells stimulated with vaccinia virus, subsequent to negative selection for reactivity to B, could not be shown to interact with virus-infected cells of type B. Either there is no significant T-cell repertoire for recognition of virus associated with an H-2 determinant not encountered during ontogeny, or such T cells are also alloreactive and are removed during filtration.
D000818:Animals; D000956:Antigens, Viral; D003429:Cross Reactions; D003602:Cytotoxicity, Immunologic; D000939:Epitopes; D005347:Fibroblasts / Q000276:immunology; D006183:H-2 Antigens; D007108:Immune Tolerance; D007156:Immunologic Memory; D009980:Influenza A virus / Q000276:immunology; D051379:Mice; D011828:Radiation Chimera; D013601:T-Lymphocytes / Q000276:immunology*; D014616:Vaccinia virus / Q000276:immunology
D000956:Antigens, Viral; D000939:Epitopes; D006183:H-2 Antigens