Rapid purification of peanut agglutinin by sialic acid-less fetuin-sepharose column.
A simple purification of peanut agglutinin (PNL) with a sialic acid-less fetuin-Sepharose column is described. Immunofluorescence studies show PNL to be a suitable surface marker of potential use in the study of subpopulations of thymocytes and T cells.
D000818:Animals; D010367:Arachis; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D006386:Hemagglutination Tests; D037102:Lectins / Q000302:isolation & purification*; D008198:Lymph Nodes / Q000276:immunology; D051379:Mice; D009439:Neuraminidase / Q000494:pharmacology; D037121:Plant Lectins; D011134:Polysaccharides / Q000494:pharmacology*; D011817:Rabbits; D012685:Sepharose / Q000494:pharmacology*; D012794:Sialic Acids; D013601:T-Lymphocytes / Q000276:immunology; D000509:alpha-Fetoproteins / Q000494:pharmacology*
D037102:Lectins; D037121:Plant Lectins; D011134:Polysaccharides; D012794:Sialic Acids; D000509:alpha-Fetoproteins; D012685:Sepharose; D009439:Neuraminidase