Incorporation of double-labelled valine into delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine by Penicillium chrysogenum.
The incorporation of valine into the LLD-tripeptide, delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine, a precursor of penicillin, was studied by incubating mycelial mats of Penicillium chrysogenum Wis. 49-2105 with double labelled valines. L-valine was incorporated into the LLD-tripeptide without formation of an alpha, beta-didehydrovaline intermediate. Intact D-valine was not incorporated into the LLD-tripeptide.
D002250:Carbon Radioisotopes; D007553:Isotope Labeling; D009842:Oligopeptides / Q000096:biosynthesis*; D010407:Penicillium / Q000378:metabolism*; D010408:Penicillium chrysogenum / Q000378:metabolism*; D014316:Tritium; D014633:Valine / Q000378:metabolism*
D002250:Carbon Radioisotopes; D009842:Oligopeptides; D014316:Tritium; D014633:Valine