Uterine protein synthesis during the early stages of pregnancy in the rat.
The synthesis of uterine-soluble proteins during early pregnancy in the rat has been examined by means of dual-isotope labelling techniques and subsequent electrophoretic analysis. A protein of similar electrophoretic mobility to the uterine oestrogen-induced protein was observed, and synthesis of this 'presumptive induced protein' was maximal on Day 4 and Day 6 of pregnancy but low on day 5. Pregnancy associated protein synthesis was observed in many regions on polyacrylamide gels, including the beta-lipoprotein, alpha2-macroglobulin, post-transferrin and albumin regions. Synthesis of the post-transferrin species rapidly increased from Day 4 to reach a maximum on Day 6 in the implantation tissue. The temporal pattern of synthesis of post-transferrin protein and and of 'presumptive induced region' suggests involvement in the processes of cell proliferation and decidualization.
D000418:Albumins / Q000096:biosynthesis; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D047108:Embryonic Development; D005260:Female; D007930:Leucine / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000096:biosynthesis; D011228:Prealbumin / Q000096:biosynthesis; D011247:Pregnancy; D011270:Pregnancy, Animal; D014176:Protein Biosynthesis; D051381:Rats; D014168:Transferrin / Q000096:biosynthesis; D014599:Uterus / Q000378:metabolism*; D000511:alpha-Macroglobulins / Q000096:biosynthesis
D000418:Albumins; D008077:Lipoproteins, LDL; D011228:Prealbumin; D014168:Transferrin; D000511:alpha-Macroglobulins; D007930:Leucine