Combination of metallic impregnation and autoradiography of brain sections. A method for differentiation of proliferating glial cells in the brain of adult rats and mice.
After labeling with 14C-thymidine, frozen sections or paraffin sections of the brain of adult mice or rats were first stained by metallic impregnation and then coated with chrome alum gelatine and with an emulsion layer of about 10 micron. On the autoradiographs 14C-tracks are readily recognized above labelled astrocytes or oligodendrocytes, and these can be well discriminated, if the sections are processed by the silver carbonate method of Rio-Hortega. In contrast, no labelling is obtained, if the gold chloride sublimate method of Cajal is applied.
D000818:Animals; D001253:Astrocytes / Q000166:cytology; D001345:Autoradiography; D001921:Brain / Q000166:cytology*; D002455:Cell Division; D051379:Mice; D009457:Neuroglia / Q000166:cytology*; D009836:Oligodendroglia / Q000166:cytology; D051381:Rats; D013194:Staining and Labeling / Q000379:methods*