title: DNA complementary to parathyroid mRNA directs synthesis of pre-proparathyroid hormone in a linked transcription-translation system.
abstract: DNA complementary in sequence to the messenger RNA for pre-proparathyroid hormone was synthesised using reverse transcriptase. In a linked transcription-translation system using RNA polymerase and cell-free extract from wheat germ, the DNA directed the synthesis of a protein identified as pre-proparathyroid hormone by N-terminal sequencing and by electrophoretic and immunologic criteria.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002474:Cell-Free System; D004247:DNA / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D000939:Epitopes; D005796:Genes; D008970:Molecular Weight; D010281:Parathyroid Hormone / Q000096:biosynthesis* / Q000276:immunology; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000096:biosynthesis* / Q000276:immunology; D012333:RNA, Messenger / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D000939:Epitopes; D010281:Parathyroid Hormone; D011498:Protein Precursors; D012333:RNA, Messenger; D004247:DNA; D012321:DNA-Directed RNA Polymerases