Effect of periodate oxidation on specific activities and carbohydrate components of human blood group N- and M-specific glycoproteins and glycopeptides.
Mild as well as strong periodate oxidation of isolated erythrocyte N and M glycoproteins and glycopeptides gave extensive to complete destruction of N-specificities as measured with Vicia graminea extracts and of N- as well as M- activities determined with all but 1 of 8 animal anti-N and 13 anti-M sera. Results with human antisera differed somewhat, while the specificity of mildly oxidized N-glycoprotein was completely destroyed as determined with all 8 human anti-N sera used, that of strongly oxidized N-active substance was completely inactivated towards one of the human antisera; the remainder showed 63--94% destruction, and 2 sera indicated no effect of oxidation. Similarly, while 10 of 14 human anti-M indicated complete inactivation of M-specific glycoproteins and glycopeptides after mild or strong oxidation, 2 showed partial inactivation and 2 human anti-M sera showed no inactivating effect of oxidation. The most relevant findings of quantitative carbohydrate analysis of periodate oxidized N- and M-specific substance were extensive transformation of N-acetylneuraminic acid (NAN) to its C8 and C1 analogues on mild oxidation and pronounced destruction of NAN and its analogues on strong oxidation; however, some intact NAN always remained. In all instances galactose (Gal) was destroyed to a much larger extent in N-derived than in M-derived glycoproteins and glycopeptides.
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002241:Carbohydrates; D002417:Cattle; D000939:Epitopes; D007887:Fabaceae / Q000276:immunology; D005643:Fucose / Q000378:metabolism; D005690:Galactose; D006020:Glycopeptides / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism*; D006736:Horses; D006801:Humans; D008951:MNSs Blood-Group System; D008358:Mannose / Q000378:metabolism; D010504:Periodic Acid / Q000494:pharmacology*; D010946:Plants, Medicinal; D011817:Rabbits; D012756:Sheep; D012794:Sialic Acids / Q000378:metabolism
D000596:Amino Acids; D002241:Carbohydrates; D000939:Epitopes; D006020:Glycopeptides; D006023:Glycoproteins; D008951:MNSs Blood-Group System; D012794:Sialic Acids; D010504:Periodic Acid; D005643:Fucose; D008358:Mannose; D005690:Galactose