Relationship of pregnancy-associated alpha2-glycoprotein (alpha2-PAG) to peripheral blood leucocytes.
The high molecular weight plasma glycoprotein pregnancy-associated alpha2-glycoprotein (alpha2-PAG) can be demonstrated on the surface of blood leucocytes and is synthesized by oestrogen-stimulated cells in culture. By the use of an indirect immunofluorescence technique, pretreatment of leucocytes with anti-alpha2-PAG IgG revealed alpha2-PAG on C3 rosette-forming cells and monocytes but not on E rosettes. Lymphocytes treated with anti-alpha2-PAG showed markedly diminished ability to form Fc rosettes. A percentage ranging from 15% to 20% of leucocytes from normal adults of both sexes, pregnant and oestrogen-treated females were positive for alpha2-PAG but no obvious correlatin was observed between the incidence of alpha2-PAG bearing cells and plasma levels of the protein.
D002478:Cells, Cultured; D004967:Estrogens / Q000494:pharmacology; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007122:Immunoelectrophoresis; D007141:Immunoglobulin Fc Fragments; D007962:Leukocytes / Q000276:immunology / Q000378:metabolism*; D008297:Male; D009000:Monocytes / Q000276:immunology; D011247:Pregnancy; D012397:Rosette Formation; D013194:Staining and Labeling; D000511:alpha-Macroglobulins / Q000032:analysis / Q000494:pharmacology
D004967:Estrogens; D007141:Immunoglobulin Fc Fragments; D000511:alpha-Macroglobulins