Immunosuppressive factor(s) specific for L-glutamic acid50-L-tyosine50. IV. In vitro activity and immunochemical properties.
The responsiveness of BALB/c spleen cells to the terpolymer L-glutamic acid60-L-alanine30-L-tyrosine10 (GAT), the copolymer of L-glutamic acid50-L-tyrosine50 (GT) and the corresponding complexes with methylated bovine serum albumin (MBSA) has been studied in vitro in a modified Mishell-Dutton culture system. Cultures of BALB/c spleen cells respond to GAT, GAT-MBSA and GT-MBSA over a wide dose range. Contrary to in vivo findings BALB/c spleen cell cultures respond to GT in vitro although in a much narrower dose range. Lymphoid extracts from GT-primed BALB/c or B10.BR mice (GT-TSF) specifically suppress the GT-MBSA response in vitro. This culture system has allowed us to further investigate immunochemical properties of both BALB/c and B10.BR GT-TSF-GT-TSF from both strains display affinity for GT-Sepharose and bear determinants encoded by the I region of the H-2 complex. Moreover, we demonstrate that the suppressive activity from B10.BR mice is a molecule or a molecular complex which displays affinity for GT and bears determinants of the I-J subregion of the H-2k haplotype.
D000818:Animals; D002457:Cell Extracts / Q000494:pharmacology; D000939:Epitopes; D005971:Glutamates / Q000276:immunology*; D006462:Hemolytic Plaque Technique; D006649:Histocompatibility Antigens; D007164:Immunosorbents / Q000494:pharmacology; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D008745:Methylation; D051379:Mice; D008807:Mice, Inbred BALB C; D012685:Sepharose / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000276:immunology; D012995:Solubility; D013154:Spleen; D013601:T-Lymphocytes / Q000276:immunology; D014443:Tyrosine / Q000276:immunology*
D002457:Cell Extracts; D000939:Epitopes; D005971:Glutamates; D006649:Histocompatibility Antigens; D007164:Immunosorbents; D007166:Immunosuppressive Agents; D012710:Serum Albumin, Bovine; D014443:Tyrosine; D012685:Sepharose