title: The role of hydroxylation of proline in the antigenicity of basement membrane collagen.
abstract: Rabbit antibodies to bovine basement membrane collagen were used to compare the antigenic determinants of rat parietal yolk sac basement membrane [14C]procollagen with [14C]protocollagen. Basement membrane [14C]protocollagen was found to be less antigenic than basement membrane [14C]procollagen. Hydroxylation of basement membrane [14C]protocollagen, either intracellularly or in vitro with protocollagen prolyl hydroxylase, resulted in restoration of antigenicity. The difference in antigenicity observed between basement membrane [14C]procollagen and basement membrane [14C]protocollagen appeared to depend primarily upon the presence of hydroxyproline in the collagen molecule. Glucosylgalactosylhydroxylysine was found to be unimportant for antigenicity.
mesh_terms: D000818:Animals; D001485:Basement Membrane / Q000276:immunology; D002417:Cattle; D003094:Collagen / Q000276:immunology*; D000939:Epitopes; D006900:Hydroxylation; D006901:Hydroxylysine / Q000494:pharmacology; D011347:Procollagen / Q000276:immunology; D011392:Proline / Q000378:metabolism*; D011817:Rabbits; D011863:Radioimmunoassay; D051381:Rats
chemical_list: D000939:Epitopes; D011347:Procollagen; D006901:Hydroxylysine; D003094:Collagen; D011392:Proline