Inhibition of recovery from bleomycin-induced potentially lethal damage.
Mammalian cells are able to recover from bleomycin (Bleo)-induced potentially lethal damage. We examined the influences of inhibitors of DNA, RNA, or protein synthesis on that recovery. Cytosine arabinoside and cycloheximide did not inhibit recovery from Bleo-induced damage; however, actinomycin D (Act D) did inhibit it. At a dose that produced minimal cell killing and inhibited only RNA synthesis, inhibition of recovery by Act D was immediate and complete.
D000963:Antimetabolites / Q000494:pharmacology*; D001761:Bleomycin / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D003561:Cytarabine / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D003609:Dactinomycin / Q000494:pharmacology; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis
D000963:Antimetabolites; D003561:Cytarabine; D001761:Bleomycin; D003609:Dactinomycin; D012313:RNA; D004247:DNA; D003513:Cycloheximide