Establishment and characterization of a new human urinary bladder carcinoma cell line (PS-1).
An epithelioid cell line (PS-1) has been established from a transitional cell cancer derived from human urinary bladder. Subcutaneous injection of the epithelioid cells into weanling athymic nude mice induced solid tumors histologically similar to the original tumor. A cell line was also established from a tumor induced in the athymic nude mouse (PS-1, T-1). Both cell lines exhibited essentially identical growth characteristics and formed a monolayer growth of epithelioid cells in culture. Electron microscopic studies confirmed epithelioid morphology. No fibroblastoid elements were observed. Chromosomal analysis revealed heteroploidy with persistent marker chromosomes; all cells contained a Y chromosome. The presence of tumor-specific antigen(s) in PS-1 cells was suggested by microcytotoxicity assays with peripheral allogeneic lymphocytes from other transitional cancer cell patients. Sera of urinary bladder cancer patients reacted with nuclear antigens of the established cells.
D000818:Animals; D002295:Carcinoma, Transitional Cell / Q000235:genetics / Q000276:immunology / Q000648:ultrastructure; D002460:Cell Line; D002871:Chromosome Banding; D002877:Chromosomes, Human; D003601:Cytotoxicity Tests, Immunologic; D005455:Fluorescent Antibody Technique; D006801:Humans; D007065:Idoxuridine / Q000494:pharmacology; D007621:Karyotyping; D008722:Methods; D051379:Mice; D008819:Mice, Nude; D008854:Microscopy, Electron; D013048:Specimen Handling; D013194:Staining and Labeling; D001749:Urinary Bladder Neoplasms / Q000235:genetics / Q000276:immunology / Q000648:ultrastructure
D007065:Idoxuridine