Mechanism of T-cell suppression in tolerance to HGG: arguments in favor of a T-cell-T-cell interaction.
The specific soluble factor from suppressor T cells of CBA mice rendered tolerant to human gammaglobulin was further investigated. Immunochemical analysis proved the low zone tolerance (LZT) factor to be similar as the one described in high zone (HZT), e. g. absorbed by anti-Iak or IGG but not anti-HGG or anti-IgG. Molecular weight was ascribed as 45--55,000 by Sephadex chromatography and "Amicon" ultrafiltration. The HZT factor acts only if administered early after challenge, and on T cell as judged by double transfer experiments, suggesting impairment of early T-cell function (helper?) by suppressor cells.
D000818:Animals; D000937:Antigen-Antibody Reactions; D006801:Humans; D007108:Immune Tolerance / Q000528:radiation effects; D007163:Immunosorbent Techniques; D007700:Kinetics; D051379:Mice; D008808:Mice, Inbred CBA; D008970:Molecular Weight; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism / Q000528:radiation effects; D014965:X-Rays; D005719:gamma-Globulins / Q000276:immunology*
D005719:gamma-Globulins