Biological and biochemical properties of Nonidet P40-solubilized and partially purified tumor-specific antigens of the transplantation type from plasma membranes of a methylcholanthrene-induced sarcoma.
Tumor-specific transplantation antigen (TSTA) was solubilized from cell membranes of sarcoma Meth-A with non-ionic detergent Nonidet P40. Soluble TSTA was partially characterized by chromatographic separation and electrophoresis. The antigen responsible for tumor rejection activity had a molecular weight of approximately 70,000 daltons in the presence of detergent and an electrophoretic mobility of alpha-globulin. TSTA was well separated from mouse histocompatibility antigen H-2 by a sequence of procedures, including gel filtration, lectin affinity chromatography, column electrophoresis, and rechromatography on agarose, showed only three major bands on polyacrylamide gel electrophoresis. TSTA was specific for sarcoma Meth-A.
D000818:Animals; D000951:Antigens, Neoplasm / Q000008:administration & dosage / Q000302:isolation & purification; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D003902:Detergents; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes; D005260:Female; D006084:Graft Rejection; D006649:Histocompatibility Antigens / Q000302:isolation & purification; D008748:Methylcholanthrene; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D009368:Neoplasm Transplantation; D011863:Radioimmunoassay; D012513:Sarcoma, Experimental / Q000139:chemically induced / Q000276:immunology*; D012995:Solubility; D014185:Transplantation, Isogeneic; D001613:beta 2-Microglobulin / Q000032:analysis
D000951:Antigens, Neoplasm; D003902:Detergents; D000939:Epitopes; D006649:Histocompatibility Antigens; D001613:beta 2-Microglobulin; D008748:Methylcholanthrene