The expression of histocompatibility-2 antigens on hemopoietic stem cells.
Antisera directed against histocompatibility-2 antigens of the mouse suppress the formation of spleen colonies by pluripotent hemopoietic stem cells. Of antisera which are specific for subregions of the H-2 complex, only those which contain anti-H-2K or anti-H-2D activity are effective. Specific anti-Ia sera do not react with the stem cell. If the titer of the CFU-s suppressive effect of the antisera is compared to their toxicity to spleen lymphocytes, it can be concluded that CFU-s express antigens in the same density as spleen lymphocytes. The amount of H-2 antigens expressed on the surface of CFU-s is independent of its rate of proliferation.
D000818:Animals; D002455:Cell Division; D002470:Cell Survival; D002999:Clone Cells; D003114:Colony-Forming Units Assay; D000939:Epitopes; D006183:H-2 Antigens; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D007106:Immune Sera; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D010206:Papain / Q000494:pharmacology; D013154:Spleen / Q000166:cytology
D000939:Epitopes; D006183:H-2 Antigens; D007106:Immune Sera; D010206:Papain