Studies on secretory glycoproteins in the rat exocrine pancreas. II. Identification of a fetuin: fucosyl transferase in the smooth microsomal fraction.
A fetuin, fucosyl transferase has been identified in the smooth microsomal fraction from the rat exocrine pancreas. This enzyme is involved in the glycosylation of secretory proteins and is bound to membranes, predominantly of the Golgi complex. Optimal in vitro conditions for the assay of the enzyme activity were established: a pH of 5.5-6.0, a temperature of 21 degrees C and concentrations of Mg++ at 5.0 mM and ATP at 2.0 mM.
D000255:Adenosine Triphosphate; D000818:Animals; D006023:Glycoproteins / Q000032:analysis*; D006056:Golgi Apparatus / Q000201:enzymology; D006154:Guanosine Diphosphate Fucose; D006602:Hexosyltransferases / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D008274:Magnesium; D008297:Male; D008861:Microsomes / Q000201:enzymology*; D010179:Pancreas / Q000201:enzymology*; D051381:Rats; D013696:Temperature; D000509:alpha-Fetoproteins
D006023:Glycoproteins; D000509:alpha-Fetoproteins; D006154:Guanosine Diphosphate Fucose; D000255:Adenosine Triphosphate; D006602:Hexosyltransferases; D008274:Magnesium