title: Serum antibodies to herpesvirus early antigens in patients with cervical carcinoma determined by anticomplement immunofluorescence technique.
abstract: Sera from patients with carcinoma of the uterine cervix were used with the anticomplement immunofluorescence (ACIF) technique to develop a simple procedure for detection of antibodies to herpes simplex virus type 2 (HSV-2) early antigens. Test cells used in the ACIF assay were HEp-2 cells infected with HSV-2 sequentially treated with inhibitors of protein and of DNA SYNTHESIS. The cells were first treated for 7 h with cycloheximide (100 mug/ml) and then for 3 h with hydroxyurea (150 mug/ml). In this assay serum titers giving more than 21.5% ACIF-positive cells correlated to invasive carcinoma of the cervix. Using these criteria 18 out of 43 patients with invasive carcinoma, none of 8 patients with carcinoma in situ, and only 1 of 43 controls, were diagnosed as positive. Thus an elevated titer of antibodies against HSV-2 antigens correlated to cervical carcinoma.
mesh_terms: D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000032:analysis; D002478:Cells, Cultured; D003168:Complement Fixation Tests; D003470:Culture Media; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D000939:Epitopes; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D006918:Hydroxyurea / Q000494:pharmacology; D018139:Simplexvirus / Q000276:immunology*; D002583:Uterine Cervical Neoplasms / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D003470:Culture Media; D000939:Epitopes; D003609:Dactinomycin; D003513:Cycloheximide; D006918:Hydroxyurea