Human gene mapping using an X/autosome translocation.
Human fibroblasts containing a translocation between the X chromosome and chromosome 15 were fused with the 6-thioguanine-resistant mouse cell line, IR. Resulting hybrids, selected in HAT medium, retained the X/15 chromosome. Hybrids which were counterselected in 6-thioguanine lost this chromosome. The X-linked markers glucose-6-phosphate dehydrogenase (G6PD), phosphoglycerate kinase (PGK), and hypoxanthine phosphoribosyl transferase (HPRT), and the non-X-linked markers pyruvate kinase (PKM2) mannose phosphate isomerase (MPI), N-acetyl hexosaminidase A (HEXA) and beta2-microglubulin (beta2-m) all segregated in concordance with the X/15 translocation chromosome. The latter markers have been assigned to chromosome 15. Selection against the X/15 chromosome was done using antihuman beta2-m serum. Electrophoretic and immunochemical analyses of the N-acetyl hexosaminidases A and B in these hybrids were performed.
D000118:Acetylglucosaminidase / Q000096:biosynthesis*; D001609:Beta-Globulins / Q000096:biosynthesis*; D002238:Carbohydrate Epimerases / Q000096:biosynthesis*; D002874:Chromosome Mapping; D002901:Chromosomes, Human, 13-15; D005796:Genes; D008040:Genetic Linkage; D006596:Hexosaminidases / Q000096:biosynthesis*; D006801:Humans; D006822:Hybrid Cells; D007621:Karyotyping; D008358:Mannose; D011770:Pyruvate Kinase / Q000096:biosynthesis*; D012730:Sex Chromosomes; D013403:Sugar Phosphates; D014178:Translocation, Genetic; D001613:beta 2-Microglobulin / Q000096:biosynthesis*
D001609:Beta-Globulins; D013403:Sugar Phosphates; D001613:beta 2-Microglobulin; D011770:Pyruvate Kinase; D006596:Hexosaminidases; D000118:Acetylglucosaminidase; D002238:Carbohydrate Epimerases; D008358:Mannose