Selective effects of anti-Ia serum and complement treatment upon polyclonal B lymphocyte responses to dextran sulfate and lipopolysaccharide.
Subpopulations of B lymphocytes have been shown to vary in their expression of Ia alloantigens and polyclonal responsiveness to thymic independent antigens. We have demonstrated that the polyclonal B cell antibody response to dextran sulfate is less sensitive to removal of Ia-positive cells than is the response to LPS. This is a consistent finding whether alloantibody and complement (C) pretreatment is directed toward cells bearing Ia antigens coded for by the entire I region or by the I-A or I-E subregions. Heterogeneity appears to exist within the dextran sulfate-sensitive population in that using high antibody; cell ratios during antibody and C-mediated cell selection results in an inhibition of the proliferative but not the antibody response. This result may indicate a differential expression of Ia antigens on dextran sulfate-sensitive B cells that respond by proliferation versus those cells that produce antibody. Alternatively, proliferative responses to dextran sulfate may be more dependent upon Ia-positive accessory cells than is the polyclonal antibody response.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D002999:Clone Cells / Q000276:immunology; D003165:Complement System Proteins / Q000276:immunology*; D003911:Dextrans / Q000494:pharmacology*; D000939:Epitopes; D005260:Female; D006462:Hemolytic Plaque Technique; D006649:Histocompatibility Antigens / Q000276:immunology*; D007106:Immune Sera / Q000494:pharmacology*; D007518:Isoantibodies / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D013997:Time Factors
D003911:Dextrans; D000939:Epitopes; D006649:Histocompatibility Antigens; D007106:Immune Sera; D007518:Isoantibodies; D008070:Lipopolysaccharides; D003165:Complement System Proteins