Measurement of immunoperoxidase activity. A rapid and reproducible immunoassay for quantitation of cellular antigens.
A procedure is described making it possible to obtain quantitative data with the immunohistochemical peroxidase anti--peroxidase (PAP) technique. After applying PAP on fixed fibroblast preparations, peroxidase enzymic activity is measured on solubilized immune-complexes. The results show this technique to be sensitive and reproducible. Differences in the amounts of the same antigen (alpha2-macroglobulin) are detected in differently treated fibroblast preparations. The value of the technique for the demonstration of cellular antigens is discussed.
D005347:Fibroblasts / Q000032:analysis; D007124:Immunoenzyme Techniques; D014404:Tubulin / Q000032:analysis; D000511:alpha-Macroglobulins / Q000032:analysis
D014404:Tubulin; D000511:alpha-Macroglobulins