[The macrophage disappearance reaction in guinea pigs sensitized with bovine gamma globulin or human scrum albumin (author's transl)].
The macrophage disappearance reaction (MDR) is a suitable test for detection of cell mediated immunity against bovine gamma globulin (BGG) and human serum albumin (HSA) in guinea pigs. The MDR is a technical simple, good manipulable, and quantifiable test. The optimal test conditions for the antigens BGC and HSA are the following: Peritoneal exudat cells (PEC) were stimulated with paraffin oil. On the 5th day after receiving oil the animals were injected with 80 microgram BGG or 30 microgram HSA i.p. 5 hours later the PEC were harvested and counted. With the MDR it is possible to detect differences with respect to degree of cell-mediated immunity. Supernatants of sensitized lymphocytes produces the MDR too.
D000818:Animals; D000941:Antigens; D001202:Ascitic Fluid / Q000166:cytology; D002417:Cattle; D002478:Cells, Cultured; D005260:Female; D006168:Guinea Pigs; D006801:Humans; D007111:Immunity, Cellular; D007158:Immunologic Techniques; D008214:Lymphocytes / Q000276:immunology; D008264:Macrophages / Q000276:immunology*; D008297:Male; D012709:Serum Albumin / Q000276:immunology*; D005719:gamma-Globulins
D000941:Antigens; D012709:Serum Albumin; D005719:gamma-Globulins
Die Makrophagen-Schwund-Reaktion zum Nachweis zellvermittelter Immunität gegen Rindergammaglobulin und Humanserumalbumin bei Meerschweinchen.