Histochemical and morphological evaluation of cerebral cortex of newborn rat in the course of joint ethanol and pyrazole administration.
The object of the study was the cerebral cortex of newborn rat. Tissue material was collected from the 2-nd to the 8-th hour after birth both from control animals and newborns whose mothers had been given both ethanol and pyrazole throughout gestation period, and also either ethanol alone or pyrazole. The drug were administered by gastric tube, in doses: ethanol 8.0 g/kg body weight, pyrazole 36 mg/kg body weight. Histochemical studies revealed variation in the intensity of reaction of the respiratory enzymes during examination of the cortex of newborns whose mothers had been fed with ethanol alone, or pyrazole alone. A strong inhibition of enzymatic reaction was observed in the examined cerebral cortex of newborns after a joint ethanol and pyrazole administration. Morphological studies showed an inhibition of maturing process of the cerebral cortex cells of animals treated with ethanol alone, whereas the cerebral cortex of animals whose mothers had been given both ethanol and pyrazole, presented symptoms allowing to diagnose encephalitis congenita symptomatica.
D000818:Animals; D002540:Cerebral Cortex / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D004950:Esterases / Q000032:analysis; D000431:Ethanol / Q000494:pharmacology*; D005260:Female; D005993:Glycerolphosphate Dehydrogenase / Q000032:analysis; D006651:Histocytochemistry; D007521:Isocitrate Dehydrogenase; D011247:Pregnancy; D011720:Pyrazoles / Q000494:pharmacology*; D051381:Rats; D013194:Staining and Labeling; D013385:Succinate Dehydrogenase / Q000032:analysis
D011720:Pyrazoles; D000431:Ethanol; D005993:Glycerolphosphate Dehydrogenase; D007521:Isocitrate Dehydrogenase; D013385:Succinate Dehydrogenase; D004950:Esterases