[Effects of serums and plasmas of various species on a protease of Staphylococcus aureus. Human alpha2-macroglobulin as an inhibitor (author's transl)].
Serums and plasmas from various animals and man inhibited 85-100% of the activity of a purified protease from Staphylococcus aureus, strain M 135 (table 1). The inhibitory effects of corresponding serums and plasmas were approximately equal. The inhibition in human serum was caused by alpha2-macroglobulin. On the other hand, alpha1-antitrypsin proved to be ineffective (table 2). The complex alpha2-macroglobulin-protease could be separated by vertical polyacrylamide discelectrophoresis. Immunelectrophoretic analysis revealed no changes by the staphylococcal protease neither on alpha2-macroglobulin, nor on other human serum proteins. Only fibrinogen was split into at least 2 components (fig. 1).
D000818:Animals; D001798:Blood Proteins / Q000494:pharmacology*; D005340:Fibrinogen / Q000378:metabolism; D006801:Humans; D007122:Immunoelectrophoresis; D010447:Peptide Hydrolases / Q000378:metabolism; D011480:Protease Inhibitors; D013045:Species Specificity; D013211:Staphylococcus aureus / Q000201:enzymology*; D000511:alpha-Macroglobulins / Q000494:pharmacology*
D001798:Blood Proteins; D011480:Protease Inhibitors; D000511:alpha-Macroglobulins; D005340:Fibrinogen; D010447:Peptide Hydrolases
Wirkungen von Seren und Plasmen verschiedener Spezies auf eine Protease von Staphylococcus aureus. Humanes alpha2-Makroglobulin als Inhibitor.