Simulation and prevention of retrovirus--specific reactions by mycoplasmas.
From human mycosis fungoides tumor-derived cell lines, Mycoplasma hyorhinis was isolated. This mycoplasma shared the following characteristics with retroviruses: uptake of 3H-uridine, but not of 3H-thymidine in cell culture; banding at 1.16 g/ml sucrose density and partial shift to retrovirus core density position (approximately equal to 1.24 g/ml) after detergent treatment; incorporation of 3H-TMP into high molecular weight material in standard reverse transcriptase assays with the template-primer poly (A) . (dT)12. On the other hand, the specific reverse transcriptase reaction of retroviruses with poly(A) . (dT)12 and poly(C) . (dG) approximately 16 was almost completely abolished in the presence of the mycoplasma. Thus, M. hyorhinis may interfere with identification and isolation procedures for retroviruses.
D002460:Cell Line; D002499:Centrifugation, Density Gradient; D006801:Humans; D009174:Mycoplasma / Q000302:isolation & purification* / Q000378:metabolism; D009182:Mycosis Fungoides / Q000382:microbiology; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D012190:Retroviridae / Q000302:isolation & purification* / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D014529:Uridine / Q000378:metabolism
D012194:RNA-Directed DNA Polymerase; D013936:Thymidine; D014529:Uridine