[Rapid detection of viruses by electron microscopy. Interest of negative staining for the diagnosis of some skin lesions of viral origin (author's transl)].
Viral particles can be visualized by electron microscopy using negative staining. Such an approach, widely used in research, provides a method for detecting virions which are present in clinical specimens. It is considered to be the method of choice in cases with suspicion of smallpox. Direct visualization has been systematically applied for the diagnosis of viral diseases in dermatology. Negative staining by pseudoreplication appears to be simple, rapid and efficient. It was thus possible to detect viruses in vesicle fluids, in scrapings and in crusts. During the course of this study 66 cases have been analyzed and viruses have been demonstrated in 49. If a skin lesion seems to be of viral origin, direct examination may confirm the clinical impression.
D006801:Humans; D008854:Microscopy, Electron; D012874:Skin Diseases, Infectious / Q000473:pathology*; D013194:Staining and Labeling; D014771:Virion / Q000648:ultrastructure; D014777:Virus Diseases / Q000473:pathology*; D014780:Viruses / Q000648:ultrastructure
Détection rapide des virus au microscope électronique. Intérêt de la méthode de coloration négative pour le diagnostic de certaines dermatoses d'origine virale.