Structure of antigenic determinants in the N-terminal region of dermatosparactic sheep procollagen type I.
About half of the rabbit antisera raised against type-I procollagen, p alpha 1(I) chain or nonreduced procollagen peptides reacted in a radioimmunoassay with the reduced form of peptide Col 1, which  comprises the whole non-collagenous region at the N-terminus of procollagen. Proteolytic fragments prepared from reduced peptide Col 1 were still effective inhibitors of the antibodies and allowed the localization of two antigenic determinants. The antigenically active regions have the sequences less than Glu-Glu-Glu-Gly-Gln-Gln-Glu and Gly-Asp-Thr-Gly-Pro-Arg, and are located at the N- and C-termini of the peptide respectively. Antibodies raised against reduced peptide Col 1 bind to a determinant localized in a different region of the peptide.
D000595:Amino Acid Sequence; D000818:Animals; D003095:Collagen Diseases / Q000276:immunology / Q000662:veterinary*; D000939:Epitopes; D010446:Peptide Fragments / Q000276:immunology; D011347:Procollagen / Q000276:immunology*; D011863:Radioimmunoassay; D012756:Sheep; D012757:Sheep Diseases / Q000276:immunology*
D000939:Epitopes; D010446:Peptide Fragments; D011347:Procollagen