[Immunochemical analysis of toxic protein fractions of burned skin].
As a result of hyperimmunization of rabbits with a preparation of toxic protein fractions from burned rat skin an antiserum, containing specific "antiburn" antibodies, was obtained. The antiserum cross-reacted in the precipitation reaction with proteins from normal skin and with macroglobulin fraction of normal blood serum. Purification and estimation of the antibodies specificity were carried out by means of immunoabsorbents, synthesized on the basis of CNBr-Sepharose 4B. The toxin from burned skin was shown to possess both the specific antigenic determinants and those common for the normal properties of skin and serum.
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D000918:Antibody Specificity; D002056:Burns / Q000276:immunology*; D003429:Cross Reactions; D000939:Epitopes; D005428:Flocculation Tests; D007106:Immune Sera; D007122:Immunoelectrophoresis; D007163:Immunosorbent Techniques; D008259:Macroglobulins / Q000032:analysis*; D011817:Rabbits; D051381:Rats; D012867:Skin / Q000276:immunology*; D014118:Toxins, Biological / Q000032:analysis*
D000906:Antibodies; D000939:Epitopes; D007106:Immune Sera; D008259:Macroglobulins; D014118:Toxins, Biological
Immunokhimicheskiĭ analiz toksicheskikh belkovykh fraktsiĭ obozhzhennoĭ kozhi