[A quick and inexpensive staining method for vaginal smears].
Vaginal cytology is a commonly used method for diagnosis of sexual cycle phases in the rat, but all staining techniques are time consuming. A simple, easy and fast technique is reported here, that is based upon the use of a Lugol solution that allows precise identification of the sexual cycle day of the rat. Following this technique, samples are taken at 8:00 a.m. and a smear is made on the glass slide adding one drop of Lugol solution. This preparation is covered and seen directly under the optic microscope.
D000818:Animals; D004972:Estrus Detection / Q000379:methods; D005260:Female; D051381:Rats; D013194:Staining and Labeling / Q000379:methods; D014626:Vaginal Smears / Q000379:methods*
Una tinción rápida y barata para citología vaginal.