The sodium pump of erythrocytes from patients with Duchenne muscular dystrophy: effect of ouabain on the active sodium flux and on (Na+, K+)ATPase.
Active ion exchanges across erythrocyte membrane from patients with Duchenne muscular dystrophy were studied by two methods: --erythrocyte ghosts (Na+,K+)ATPase activity and its sensibility to ouabain, using an enzymatic method; --active sodium outflux and its sensibility to ouabain using a radioisotopic method. (Na+,K+)ATPase activity and active sodium outflux were decreased in dystrophic patients compared to normal sex- and age-matched controls. Enzymatic activity and active sodium outflux were inhibited by ouabain 10(-4) M in patients and in controls. Inhibition of the active sodium outflux was lower in patients than in controls. The results are discussed in light of the literature.
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000293:Adolescent; D001693:Biological Transport, Active / Q000187:drug effects; D002648:Child; D004910:Erythrocyte Membrane / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D009136:Muscular Dystrophies / Q000097:blood*; D010042:Ouabain / Q000494:pharmacology; D012964:Sodium / Q000378:metabolism*
D010042:Ouabain; D012964:Sodium; D000251:Adenosine Triphosphatases