Ontogeny of macrophage function. I. Phagocytic activity and A-cell activity of newborn and adult mouse peritoneal macrophages.
Phagocytic activity and immune-participating A-cell activity of newborn and adult mouse macrophages were investigated under in vitro conditions. Thioglycollate medium-stimulated newborn (SNB) or adult (SA), and nonstimulated adult (NA) peritoneal macrophages were used. Immune complexes of sheep erythrocytes (SRBC), aldehyde-fixed SRBC, and latex beads were employed in phagocytosis tests. A-cell activity was estimated as the capacity to support primary or secondary responses of macrophage-deprived adult spleen cells to SRBC. Results obtained were: 1) phagocytic activity of NA macrophages was highest and that of SNB macrophages was higher than SA macrophages, 2) no difference was observed in A-cell activity for secondary response among SNB, SA, and NA macrophages, and 3) SNB macrophages were lacking in A-cell activity for primary response. These results suggest that the substantial A-cell function should be evaluated in primary responses, and that the A-cell and pahgocytic functions do not necessarily accompany each other.
D000375:Aging; D000818:Animals; D000831:Animals, Newborn; D004910:Erythrocyte Membrane / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D006801:Humans; D007700:Kinetics; D008264:Macrophages / Q000276:immunology*; D008297:Male; D051379:Mice; D008806:Mice, Inbred AKR; D008809:Mice, Inbred C3H; D010587:Phagocytosis; D013864:Thioglycolates / Q000494:pharmacology; D013997:Time Factors; D014357:Trypsin / Q000494:pharmacology; D005719:gamma-Globulins / Q000276:immunology
D013864:Thioglycolates; D005719:gamma-Globulins; D014357:Trypsin