Synthesis of a fully active snake venom cardiotoxin by fragment condensation on solid polymer.
A polypeptide cardiotoxin containing 60 amino acids with 4 disulfide bonds has been synthesized by the "fragment solid-phase" method. The identity of the synthetic product with native cardiotoxin was established by chromatography on Sephadex G-50, carboxymethyl-cellulose column chromatography, thin layer chromatography, disc gel electrophoresis, amino acid analysis, end group analysis, peptide mapping, circular dichroism spectra, and four biological tests.
D000595:Amino Acid Sequence; D001681:Biological Assay; D004546:Elapid Venoms / Q000138:chemical synthesis* / Q000276:immunology / Q000494:pharmacology; D000939:Epitopes; D011108:Polymers; D012995:Solubility; D013329:Structure-Activity Relationship
D004546:Elapid Venoms; D000939:Epitopes; D011108:Polymers