Evidence for recombination between N- and B-tropic murine leukemia viruses.
After co-infection of Sc-1 cells with N- and B-tropic murine leukemia viruses that differ in their XC plaque morphology, Hopkins et al. (1976) obtained viruses, designated XLP-N, that appeared to be recombinants, since they possess the N-tropism of one parent and the XC plaque morphology of the other (the B-tropic virus) parent. Here we present evidence, based on antigenicity and electrophoretic mobility, that some clonal isolates of XLP-N have inherited gp70 gene of their B-tropic virus parent. In addition to providing evidence that XLP-N viruses are recombinants, the fact that an N-tropic virus may apparently possess a gp70 derived from a B-tropic virus provides evidence, which is in agreement with the findings of others (Huang et al., 1973; Krontiris et al., 1973) that the N- or B-tropism of murine leukemia virus does not reside in gp70.
D002460:Cell Line; D003601:Cytotoxicity Tests, Immunologic; D000939:Epitopes; D006023:Glycoproteins / Q000276:immunology; D009052:Leukemia Virus, Murine / Q000254:growth & development / Q000276:immunology; D011995:Recombination, Genetic; D014764:Viral Proteins / Q000276:immunology
D000939:Epitopes; D006023:Glycoproteins; D014764:Viral Proteins