Light and electron histochemistry of phosphoamidase with p-chloranilidophosphonic acid and with cyclophosphamide (endoxan).
The original metal-salt technique of Gomori (1948a) employing p-chloranilidophosphonic acid as a substrate for the demonstration of the activity of phosphoamidase has been used with varying success by a number of investigators for light microscopy. Cyclophosphamide (endoxan) which is a cytotoxic drug known to activate phosphoamidase and other lysosomal enzymes in neoplasm (Grillo, 1971) is proposed as another substrate for the enzyme for both light and electron microscopy.
D000813:Anilides; D002051:Burkitt Lymphoma / Q000201:enzymology*; D004396:Coloring Agents; D003520:Cyclophosphamide / Q000494:pharmacology; D004789:Enzyme Activation; D006801:Humans; D006867:Hydrolases / Q000032:analysis* / Q000378:metabolism; D009943:Organophosphorus Compounds; D013194:Staining and Labeling / Q000379:methods*
D000813:Anilides; D004396:Coloring Agents; D009943:Organophosphorus Compounds; D003520:Cyclophosphamide; D006867:Hydrolases