Relationship between E receptors and a T-specific surface antigen on human T cells.
The aim of this study was the delineation of different antigenic surface determinants on the surface of adult peripheral T cells by means of a specific horse anti-human T-cell serum (ATS). It was shown that this serum reacts both with E receptors and (an) additional T antigen(s). While E receptors showed the already known susceptibility to trypsin, T antigens (as demonstrated in cytotoxicity tests) were resistant to trypsinization even at high concentration. Incubation of the trypsinized peripheral blood lymphocytes (PBL) in 5 per cent CO2 allowed the resynthesis of E-receptors. High concentrations of ATS (without complement) significantly inhibited rosette-formation. This suggests a close steric relationship between E receptors and T antigen(s). However, absorptions of ATS with trypsinized PBL left the E-rosette inhibitory capacity unaltered. Treatment of PBL with ATS in appropriate dilutions and indirect immunofluorescence tests under capping conditions followed by conventional rosette procedures showed that the E receptor and T antigens are separately mobile within the T-cell membrane.
D000941:Antigens; D000961:Antilymphocyte Serum / Q000494:pharmacology; D001666:Binding Sites, Antibody; D002462:Cell Membrane / Q000276:immunology*; D000939:Epitopes; D004912:Erythrocytes / Q000276:immunology; D007104:Immune Adherence Reaction; D013601:T-Lymphocytes / Q000276:immunology*; D014357:Trypsin / Q000494:pharmacology
D000941:Antigens; D000961:Antilymphocyte Serum; D000939:Epitopes; D014357:Trypsin