In search of alpha 1-microglobulin on the lymphocyte surface.
alpha 1-Microglobulin was found by immunofluorescence not to be associated with human lymphoid and nonlymphoid cell lines. No accumulation of alpha 1-microglobulin was detected in culture media of these cell lines. A weak membrane fluorescence with anti-alpha 1-microglobulin on peripheral lymphocytes could not be blocked by the purified protein. No release of alpha 1-microglobulin into the growth medium was seen by normal cultured leukocytes. Treatment of normal lymphocytes, erythrocytes, and various cell lines with solubilization techniques did not yield any alpha 1-microglobulin. alpha 1-Microglobulin and protein HC display immunologic and biochemical identity. However, anti-protein HC stained almost all of the tested cell lines and normal lymphocytes. Blocking experiments with the purified protein were not successful. Antibodies reacting with a minor impurity (50,000 d) in the alpha 1-microglobulin or protein HC preparations could be absorbed from anti-alpha 1-microglobulin with normal leukocytes and a lymphoid cell line.
D000042:Absorption; D000510:Alpha-Globulins / Q000276:immunology*; D000595:Amino Acid Sequence; D000818:Animals; D000941:Antigens / Q000276:immunology; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000276:immunology*; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D005779:Immunodiffusion; D008214:Lymphocytes / Q000276:immunology*; D008565:Membrane Proteins / Q000276:immunology*; D011817:Rabbits; D011863:Radioimmunoassay; D012995:Solubility
D000510:Alpha-Globulins; D000941:Antigens; D006023:Glycoproteins; D007106:Immune Sera; D008565:Membrane Proteins