title: Human beta-globin messenger RNA. III. Nucleotide sequences derived from complementary DNA.
abstract: Sequences of human beta-globin mRNA were determined by analysis of complementary DNA. beta-mRNA was transcribed into double-stranded cDNA by RNA-dependent DNA polymerase. cDNA was cut by restriction endonucleases and the fragments were terminally labeled by means of polynucleotide kinase and [gamma-32P]ATP. After purification, fragments were degraded by snake venom phosphodiesterase. Alternatively single-stranded [32P]cDNA was prepared by transcription in the presence of [alpha-32P]dCTP and actinomycin D; the product was digested by endonuclease IV and degraded by snake venom phosphodiesterase. cDNA tracts obtained by both labeling methods enabled us to construct a sequence for the translated and 3'-terminal untranslated regions of human beta-mRNA.
mesh_terms: D000755:Anemia, Sickle Cell; D001483:Base Sequence; D004247:DNA; D004262:DNA Restriction Enzymes; D003609:Dactinomycin; D003851:Deoxyribonucleases; D004720:Endonucleases; D005914:Globins / Q000096:biosynthesis*; D006801:Humans; D009838:Oligodeoxyribonucleotides; D010727:Phosphoric Diester Hydrolases; D011116:Polynucleotide 5'-Hydroxyl-Kinase; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase; D013789:Thalassemia; D014158:Transcription, Genetic
chemical_list: D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D003609:Dactinomycin; D005914:Globins; D004247:DNA; D011116:Polynucleotide 5'-Hydroxyl-Kinase; D012194:RNA-Directed DNA Polymerase; D003851:Deoxyribonucleases; D004720:Endonucleases; D004262:DNA Restriction Enzymes; D010727:Phosphoric Diester Hydrolases