Search for Epstein-Barr and type C oncornaviruses in systemic lupus erythematosus.
Lymphoblastoid cell lines were derived from patients with active systemic lupus erythematosus by allowing spontaneous transformation of peripheral B lymphocytes (B cells) harboring endogenous Epstein-Barr virus or by superinfecting peripheral lymphocytes with exogeneous Epstein-Barr virus. Results of extensive studies aimed at identifying type C oncornaviruses in these lymphoblastoid cells were entirely negative by electron microscopy, DNA-DNA hybridization, reverse transcriptase assays, and cocultivation experiments. These results do not support the postulated association of oncornavirus infection in human systemic erythematosus.
D001402:B-Lymphocytes; D001973:Bromodeoxyuridine; D002460:Cell Line; D004854:Herpesvirus 4, Human / Q000302:isolation & purification*; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000382:microbiology*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000648:ultrastructure; D009693:Nucleic Acid Hybridization; D012194:RNA-Directed DNA Polymerase; D012190:Retroviridae / Q000302:isolation & purification*
D012194:RNA-Directed DNA Polymerase; D001973:Bromodeoxyuridine