Demonstration of rheumatoid factor idiotypic antigens on peripheral blood B and T lymphocytes from patients with rheumatoid arthritis.
Antisera were raised against three polyclonal IgM rheumatoid factors (RF). After adequate absorptions, the antisera were rendered idiotype-specific, as assayed by haemagglutination technique. By using the anti-idiotype antisera in indirect immunofluorescence on peripheral blood lymphocytes from the patients used as donors for the immunizing RF, it was demonstrated that 3-14% of the lymphocytes were stained, and thus had membrane-bound structures with idiotypic antigens similar to those of the circulating IgM RF of the same patients. While most of these idiotype-positive lymphocyte were B lymphocytes, it was demonstrated in one patient that about 7% of the T lymphocytes also had the same idiotypic antigens.
D001172:Arthritis, Rheumatoid / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D000939:Epitopes; D005455:Fluorescent Antibody Technique; D006386:Hemagglutination Tests; D006801:Humans; D007106:Immune Sera; D007075:Immunoglobulin M / Q000032:analysis; D012217:Rheumatoid Factor; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000276:immunology*; D014357:Trypsin / Q000494:pharmacology
D000939:Epitopes; D007106:Immune Sera; D007075:Immunoglobulin M; D012217:Rheumatoid Factor; D014357:Trypsin