Extracellular antigens of Micropolyspora faeni grown in synthetic medium.
Extracellular antigens were prepared by growing Micropolyspora faeni in a new synthetic medium (SMSL), and this antigenic preparation was compared with antigens obtained by the double-dialysis method. These two preparations were tested against pooled sera from farmer's lung patients and rabbits immunized with these antigens by the agar-gel double-diffusion, immunoelectrophoresis, and two-dimensional immunoelectrophoresis methods. The immunological cross-reactivity between M. faeni strains of diverse origin and between other species of thermophilic actinomycetes associated with hypersensitivity pneumonitis was also investigated. The usefulness of two-dimensional immunoelectrophoresis in the comparison and standardization of antigens is discussed.
D000942:Antigens, Bacterial; D003429:Cross Reactions; D003470:Culture Media; D000939:Epitopes; D005203:Farmer's Lung / Q000276:immunology; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D008848:Micromonosporaceae / Q000254:growth & development / Q000276:immunology*
D000942:Antigens, Bacterial; D003470:Culture Media; D000939:Epitopes