Cytotoxicity of ionophore A23187 for basophils and other human blood cells.
The calcium ionophore A23187(A23) at concentrations exceeding 1 microgram/ml has been shown to be progressively cytotoxic for human blood basophils, neutrophils, lymphocytes, and erythrocytes. Toxicity to basophils was considered to be manifested by the increasing inability of 2-deoxyglucose (2DG) to inhibit histamine release (HR) at increasing concentrations of A23. The toxicity to neutrophils and lymphocytes was demonstrated by decreased lactate production (LP) after incubation with A23 of Ficoll-Hypaque fractions greatly enriched in each respective cell type. Red cells present in dextran-sedimented leukocytes were increasingly susceptible to lysis during washing subsequent to exposure to increasing concentrations of A23. A concentration of 4 microgram A23/ml, which is cytotoxic at 37 degrees C, produced optimal and noncytotoxic HR at 22 degrees C. It was possible to reduce A23 concentrations required for optimal HR by increasing Ca++ from 0.6 to 3 mM.
D000900:Anti-Bacterial Agents / Q000633:toxicity*; D001491:Basophils / Q000187:drug effects*; D000001:Calcimycin / Q000633:toxicity*; D002118:Calcium / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006098:Granulocytes / Q000187:drug effects; D006636:Histamine Release / Q000187:drug effects; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects; D013696:Temperature
D000900:Anti-Bacterial Agents; D000001:Calcimycin; D002118:Calcium