Activation of an endogenous C-type RNA virus in rat embryo cells after transformation by herpes simplex virus types 1 and 2.
Reverse transcriptase activity was detected in the supernatants of rat embryo fibroblast cell cultures transformed by HSV types 1 and 2 at either the sub-optimal temperature of 20 degrees C or the supra-optimal temperature of 42 degrees C. Rat cells clones which had been transformed at 20 degrees C contained higher levels of C-type virus DNA polymerase than did cell clones which had been transformed at 42 degrees C. Syncytia formation typical for C-type RNA viruses occurred at passages higher than 24. The activation of endogenous C-type RNA viruses was independent of the virus and transformation method used.
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002474:Cell-Free System; D002999:Clone Cells; D012328:RNA Viruses / Q000201:enzymology*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*; D051381:Rats; D012190:Retroviridae / Q000201:enzymology*; D018139:Simplexvirus / Q000254:growth & development*; D013696:Temperature; D014779:Virus Replication
D012194:RNA-Directed DNA Polymerase