Isolation and partial purification of the major abundant class rat seminal vesicle poly(A+)-messenger RNA.
Total poly(A(+))-RNA (poly(A(+))-RNA(tot)) was isolated from rat seminal vesicle and its size distribution determined by 70% formamide 5-25% sucrose density analysis. One major peak was resolved in the 10-13 S region and accounted for approximately 35% of the total poly(A(+))-RNA applied. Preparative 1% SDS, 5-20% linear sucrose density gradients also resolved a single major peak in the 11S region (poly(A(+))(11S). Analysis of poly(A(+))-RNA(tot) and poly(A(+))-RNA(11S) under denaturing conditions on 2% agarose gel electrophoresis demonstrated two major components in both poly(A(+))-RNA populations. Size estimations for these components are 620 and 540 NT respectively. (3)H-cDNA was made to both poly(A(+))-RNA(tot) and poly(A(+))-RNA(11S). Back-hybridization of poly(A(+))-RNA(tot) and poly(A(+))-RNA(11S) to their respective (3)H-cDNA revealed a highly abundant class representing 41% and 85% of the sequences in their respective (3)H-cDNA's. The highly abundant class corresponded to 3-5 sequences present in 30,000-50,000 copies/cell. Invitro translation of poly(A(+))-RNA(11S) resulted in two major polypeptides coded for by the 620 NT long and 540 NT long poly(A(+))-RNA respectively.Images
D000818:Animals; D001483:Base Sequence; D002645:Chickens; D005260:Female; D007700:Kinetics; D008297:Male; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D010057:Oviducts / Q000378:metabolism; D011061:Poly A / Q000302:isolation & purification*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000302:isolation & purification* / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase; D051381:Rats; D012669:Seminal Vesicles / Q000032:analysis* / Q000378:metabolism
D012333:RNA, Messenger; D011061:Poly A; D012194:RNA-Directed DNA Polymerase