Bovine leukemia virus: an exogenous RNA oncogenic virus?
Short term cultures of bovine leukemic lymphocytes release virus particles with biochemical properties of RNA oncogenic viruses. These particles, tentatively called Bovine Leukemia Virus (BLV) have a high molecular weight-reverse transcriptase complex and a density averaging 1.155 g/ml in sucrose solutions. Molecular hybridizations between BLV-3H cDNA and several viral RNAs show that BLV is not related to Mason-Pfizer Monkey Virus (MPMV) Simian Sarcoma Associated Virus (SSV-1) Feline Leukemia Virus (FeLV) or Avian Myeloblastosis Virus (AMV). Rauscher Leukemia Virus (RLV) exhibits a slight but reproducible relatednesse to BLV. The high preference of BLV reverse transcriptase for Mg++ as the divalent cation suggests that BLV might be an atypical mammalian leukemogenic type C virus. Hybridization studies using BLV 3H cDNA as a probe suggest that the DNA of bovine leukemic cells contains viral sequences that cannot be detected in normal bovine DNA.
D000818:Animals; D001483:Base Sequence; D002413:Cations, Divalent; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology; D002500:Centrifugation, Isopycnic; D004273:DNA, Neoplasm / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis; D003902:Detergents; D001909:Leukemia Virus, Bovine / Q000032:analysis / Q000201:enzymology; D008228:Lymphoma, Non-Hodgkin / Q000662:veterinary; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000032:analysis*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*; D012190:Retroviridae / Q000032:analysis; D013045:Species Specificity
D002413:Cations, Divalent; D004273:DNA, Neoplasm; D004279:DNA, Viral; D003902:Detergents; D012367:RNA, Viral; D012194:RNA-Directed DNA Polymerase