[H-2 specificity and auto-reactivity of autologous rosette-forming cells from adult thymectomized mice spleen cells].
We have previously shown that the number of autologous rosette-forming cells (A-RFC) found in adult thymectomized mice (A-Tx) spleens was 15 times higher than that found in spleens of intact controls. We have therefore investigated both the specificity of A-RFC and their relationship with autoreactivity. Immunoadsorption experiments showed that while the incubation on allogeneic monolayers did not bring any modification in A-RFC levels. This result suggests that the formation of autologous rosettes is the manifestation of a true recognition event associated with the H-2 complex. A-Tx mice spleen cells injected into syngeneic recipients induced an enlargement of the afferent popliteal lymph node (LN). Ficoll-Triosil A-RFC depleted A-Tx spleen cells lost their capacity to induce this popliteal proliferation. Conversely, A-RFC recovered spleen cell suspensions obtained from the pellet of the Ficoll Triosil gradient kept their capacity of inducing auto-reactivity. Thus, it seems that A-RFC and lymphocytes stimulating the afferent LN belong to the same sub-population.
D000375:Aging; D000818:Animals; D000939:Epitopes; D006649:Histocompatibility Antigens; D007158:Immunologic Techniques; D051379:Mice; D013154:Spleen / Q000276:immunology*; D013934:Thymectomy
D000939:Epitopes; D006649:Histocompatibility Antigens
Spécificité H-2 et autoréactivité des rosettes autologues de la rate d'animaux thymectomisés a l'age adulte