title: Low-molecular-weight RNAs of Moloney murine leukemia virus: identification of the primer for RNA-directed DNA synthesis.
abstract: The small RNAs of Moloney murine leukemia virus (M-MuLV) were fractionated into at least 15 species by two-dimensional polyacrylamide gel electrophoresis. The pattern of small RNAs is significantly different from that of Rous sarcoma virus. A subset of the virion small RNAs is associated with the genome RNA in the 70S complex. One of the associated molecules, a cellular tRNA, is tightly bound to the genome RNA and serves as the major primer for M-MuLV RNA-directed DNA synthesis in vitro.
mesh_terms: D004279:DNA, Viral / Q000096:biosynthesis*; D008970:Molecular Weight; D008979:Moloney murine leukemia virus / Q000032:analysis* / Q000201:enzymology / Q000378:metabolism; D009841:Oligonucleotides / Q000032:analysis; D012343:RNA, Transfer / Q000032:analysis* / Q000378:metabolism; D012367:RNA, Viral / Q000032:analysis* / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism
chemical_list: D004279:DNA, Viral; D009841:Oligonucleotides; D012367:RNA, Viral; D012343:RNA, Transfer; D012194:RNA-Directed DNA Polymerase