Heterogeneity in cell-associated transformation-sensitive proteins antigenically related to fibronectin.
Antiserum to purified fibronectin has been used to investigate transformation-associated heterogeneity in surface components antigenically related to fibronectin. Examination of extracts from surface-labeled rat cells by gradient gel electrophoresis revealed in "normal" cells the presence of two species with approximate molecular weights of 250,000 and 230,000, which were decreased in wild-type transformed cells. Reaction with antifibronectin serum revealed the selective precipitation of the two transformation-sensitive surface components. A similar experiment with ts-NT3-KR that expresses a normal phenotype at 37 degrees and a transformed morphology at 33 degrees did not reveal a markedly altered surface labeling pattern at both temperatures. However, reaction with antifibronectin serum did show a weak but detectable recognition of a 230,000-dalton doublet in the cells grown at 37 degrees, and immune precipitation of components in the 100,000- and 60,000-dalton region in cells grown at 33 degrees. Experiments with normal mouse cells revealed a different ratio of two fibronectin-related external proteins with similar molecular weights to those seen in normal rat cells.
D000818:Animals; D000954:Antigens, Surface; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D003429:Cross Reactions; D000939:Epitopes; D005347:Fibroblasts / Q000276:immunology*; D006023:Glycoproteins / Q000276:immunology*; D051379:Mice; D008970:Molecular Weight; D051381:Rats
D000954:Antigens, Surface; D000939:Epitopes; D006023:Glycoproteins