Differential activation of cytotoxic and suppressor T cells against syngeneic tumors in the mouse.
The cytotoxic T cell against a methylcholanthrene-induced sarcoma, S1509a, was induced in syngeneic mice by deliberate immunization with mitomycin C (MMC)-treated live tumor cells. The soluble tumor antigen (STA) extracted from the same tumor by 3 M KCl was, however, unable to induce the cytotoxic T cell upon immunization, although it was able to activate predominantly the suppressor T cell that then specifically suppressed the effect of the cytotoxic T cell against the homologous tumor. The suppressor T cell generated by STA had the same characteristics as those found in tumor-bearing animals: 1) The suppressor T cell has a very strict specificity against individual tumors; 2) The cell expresses cell surface determinants controlled by genes in the I-J subregion of the mouse H-2 complex. The activity of the cytotoxic T cell was completely inhibited by live tumor cells but not by STA, whereas that of the suppressor T cell was neutralized by STA. The results that cytotoxic and suppressor T cells are activated under different conditions, and that the antigenic determinants recognizable by these two cell types are not the same. The soluble extract contains only the determinants recognizable by the suppressor T cell, and the cytotoxic T cell can be activated only by the determinants associated with self antigen present on the surface of live tumor cells.
D000818:Animals; D002457:Cell Extracts / Q000276:immunology; D003080:Cold Temperature; D003429:Cross Reactions; D003602:Cytotoxicity, Immunologic; D000939:Epitopes; D007109:Immunity; D008213:Lymphocyte Activation; D051379:Mice; D008805:Mice, Inbred A; D008937:Mitomycins / Q000494:pharmacology; D012513:Sarcoma, Experimental / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D002457:Cell Extracts; D000939:Epitopes; D008937:Mitomycins