Radioimmunoassay procedure for quantitating bacterial antibody in human sera.
A RIA system was developed to detect antibodies in human sera against bacteria. Sonicates of Escherichia coli and Fusobacterium polymorphum were used as antigens to sensitize plastic-coated beads; antibodies to these antigens were detected with 125I-labeled antihuman globulin. Serum antibody titers against E. coli were determined by the serial dilution method; from the results the standard curve principle was applied in determining the relative amounts of antibodies against E. coli in serum samples tested at a single dilution. The coefficient of variation of the RIA procedure was less than 10%. Serum titers obtained by the RIA and indirect immunofluorescence test were compared; RIA was more sensitive, quantitative and objective. Absorption studies, using E. coli and F. polymorphum absorbentes against E. coli and F. polymorphum. This RIA procedure offers a combination of desirable advantages; it is sensitive, specific, objective, quantitative, and easy to perform.
D000907:Antibodies, Bacterial / Q000032:analysis*; D000942:Antigens, Bacterial / Q000032:analysis; D000939:Epitopes; D004926:Escherichia coli / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D005673:Fusobacterium / Q000276:immunology*; D006801:Humans; D011863:Radioimmunoassay / Q000379:methods
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D000939:Epitopes