title: Reverse transcription of turnip yellow mosaic virus RNA primed with calf-thymus DNA hydrolysate: characterization of the purified cDNA product.
abstract: Complementary DNA was transcribed from turnip yellow mosaic virus RNA, using the method of Taylor et al. (1). The purified cDNA thus obtained sedimented between 2 and 4 S and was a mostly uniform transcript of template RNA. It hybridized with a sharp transition to homologous TYMV-RNA (Crt 1/2 = 2.7 x 10(-2)), but showed a low level of hybridization (less than 5%) to the RNAs of two other tymoviruses, namely Andean potato latent virus and eggplant mosaic virus.
mesh_terms: D009189:Avian Myeloblastosis Virus / Q000201:enzymology; D004247:DNA / Q000096:biosynthesis*; D004355:Drug Stability; D006358:Hot Temperature; D007700:Kinetics; D009029:Mosaic Viruses; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000378:metabolism*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*; D013950:Thymus Gland
chemical_list: D012367:RNA, Viral; D004247:DNA; D012194:RNA-Directed DNA Polymerase