Fuorescent retrograde neuronal labeling in rat by means of substances binding specifically to adenine-thymine rich DNA.
Six fluorescent substances, binding specifically to adenine-thymine rich DNA, were injected in rat caudate-putamen. This resulted in retrograde axonal transport and fluorescent retrograde labeling of neurons in center-medium parafascicular nucleus, substantia nigra and dorsal raphe. Two of these substances, i.e. "True Blue' and 'Granular Blue', give a very striking blue fluorescent retrograde neuronal labeling. Mid-thoracic spinal injections of these two substances in rat also resulted in a pronounced retrograde fluorescent labeling of neurons of the descending brainstem pathways and of neurons in the sensori-motor cortex.
D000225:Adenine; D000818:Animals; D001923:Brain Chemistry; D004247:DNA / Q000032:analysis*; D005456:Fluorescent Dyes; D006651:Histocytochemistry; D008856:Microscopy, Fluorescence; D009474:Neurons / Q000032:analysis*; D051381:Rats; D013194:Staining and Labeling; D013941:Thymine
D005456:Fluorescent Dyes; D004247:DNA; D000225:Adenine; D013941:Thymine