[Studies on human proinsulin C-peptide radioimmunoassay method--preparation of antiserum and its specificity-- (author's transl)].
The antisera using at final dilution of 1 : 10,000 have been prepared by immunizing synthetic human proinsulin connecting peptide to rabbits for human proinsulin C-peptide radioimmunoassay. The cross reactivities of human proinsulin C-peptide derivatives with the prepared antisera were reduced by leaving amino acid residues from N terminal, although this phenomenon was a little different among antisera. Those results suggested that main antigen determinant in N terminal 31-38 of human proinsulin connecting peptide. The cross reactivities of other animal proinsulin C-peptide and other peptide hormones with the prepared antissera were not recognized at 10(3) p mole/ml.
D002096:C-Peptide / Q000032:analysis*; D003429:Cross Reactions; D000939:Epitopes; D006801:Humans; D007106:Immune Sera; D007158:Immunologic Techniques; D010455:Peptides / Q000032:analysis*; D011863:Radioimmunoassay / Q000379:methods*
D002096:C-Peptide; D000939:Epitopes; D007106:Immune Sera; D010455:Peptides