[Dependence of the cytogenetic effect on TEPA concentration in human lymphocyte culture].
The authors studied the cytogenetic action of TEPA (tris/2-methyl-1-azyridinyl) on the human lymphocyte culture. It was shown that the increase of the mutagen concentration from 0.125 to 16.0 microgram/ml the cytogenetic effect for the portion of the aberrant metaphases rose from 6.0 to 61.0%, and for the total number of ruptures - from 7.96 to 116.3. A method of finding the least effective concentration of the substance under study in comparison with control is suggested; for  TEPA it constitutes 0.120 microgram/ml. The percentage of chromatide ruptures remained constant in using different TEPA concentration and constitutes 51.72%. Cell distribution of chromosome ruptures is satisfactorily described by geometrical distribution.
D001389:Azirines / Q000494:pharmacology*; D002869:Chromosome Aberrations; D046508:Culture Techniques; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008214:Lymphocytes / Q000166:cytology*; D009153:Mutagens; D013721:Triethylenephosphoramide / Q000008:administration & dosage / Q000494:pharmacology*
D001389:Azirines; D009153:Mutagens; D013721:Triethylenephosphoramide
Zavisimost' tsitogeneticheskogo deĭstviia ot kontsentratsii TEPA v kul'ture limfotsitov cheloveka.