The induction of cytolytic T lymphocytes with specificity for p-azophenylarsonate coupled syngeneic cells.
Conditions are described for diazonium linkage of the hapten p-azophenylarsonate (Ar) to mouse lymphocytes for the purpose of induction of syngeneic cytolytic T lymphocytes. These cytolytic effector cells do not lyse target cells that are coupled with an unrelated hapten, trinitrophenyl. Cell lysis can be blocked by the addition of anti-Ar antiserum. Only those target cells that share H-2 K or D end antigens with the stimulating cell population are susceptible to lysis. Several methods were used in an attempt to demonstrate the presence of the Ar cross-reactive idiotype on CTL induced in A/J mice.
D000818:Animals; D001391:Azo Compounds / Q000276:immunology*; D003429:Cross Reactions; D003602:Cytotoxicity, Immunologic; D000939:Epitopes; D006649:Histocompatibility Antigens; D007130:Immunoglobulin Idiotypes; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes / Q000276:immunology*; D014301:Trinitrobenzenes / Q000276:immunology; D010132:p-Azobenzenearsonate / Q000276:immunology*
D001391:Azo Compounds; D000939:Epitopes; D006649:Histocompatibility Antigens; D007130:Immunoglobulin Idiotypes; D014301:Trinitrobenzenes; D010132:p-Azobenzenearsonate