Induction of high frequencies of endoreduplication in mammalian cell cultures with 33258 Hoechst and rubidazone.
Cells of the mouse L strain and the Chinese hamster CHO line were treated with 33258 Hoechst, rubidazone, and a combination of these. Recovering cell populations following the drug removal exhibited a high frequency (20--50%) of metaphases with diplochromosomes (endoreduplication), especially in the combination treatment series (up to more than 70% in the L strain). Such a procedure should be useful in probing the mechanisms of the endoreduplication process.
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001562:Benzimidazoles / Q000494:pharmacology*; D006690:Bisbenzimidazole / Q000494:pharmacology*; D002460:Cell Line; D002478:Cells, Cultured; D006224:Cricetinae; D004261:DNA Replication / Q000187:drug effects*; D003630:Daunorubicin / Q000031:analogs & derivatives* / Q000494:pharmacology; D008677:Metaphase / Q000187:drug effects; D051379:Mice; D011123:Polyploidy
D000970:Antineoplastic Agents; D001562:Benzimidazoles; D006690:Bisbenzimidazole; D003630:Daunorubicin