[Detection of cell-mediated immunity against protein antigen by the macrophage adherence inhibition test (author's transl)].
The macrophage adherence inhibition test is a suitable assay for detection of cell-mediated immunity against bovine gamma globulin (BGG) in guinea pigs. The test was performed with peritoneal cells (PC) in hemocytometer chambers. 10 mg/ml BGG were added as antigen. The washing step was performed with a burette. An average standard deviation of 5% was obtained with this washing method. Day-to -day-investigations in individual animals show differences with respect to the degree of cell-mediated immunity against BGG. Supernatants of sensitized spleen cells or lymph node cells produce adherence inhibition in PC of nonimmunized guinea pigs too.
D000818:Animals; D002448:Cell Adhesion; D005260:Female; D006168:Guinea Pigs; D007111:Immunity, Cellular; D008264:Macrophages / Q000276:immunology*; D008297:Male; D005719:gamma-Globulins
D005719:gamma-Globulins
Nachweis zellvermittelter Immunität gegen Proteinantigen mit dem Makrophagenadhärenzhemmtest.