Characterization of proteins transported at different rates by axoplasmic flow in the dorsal root afferents of rats.
Proteins synthesized by soma located in L4 dorsal root ganglia and supplied to the axonal branches extending centrally in the dorsal root and peripherally towards the sciatic nerve were analyzed for radioactivity following injections of [3H] leucine into the L4 dorsal root ganglia. All proteins located in the dorsal root and sciatic nerve were analyzed by SDS acrylamide gel electrophoresis at various times post injection. The differences in radioactivity between the dorsal root and sciatic nerve proteins were mainly quantitative and not qualitative, with many proteins of various molecular weight ranges being transported into both segments. Generally, it appears that in both axonal branches the high molecular weight proteins are transported at the highest rate, medium weights slower and low molecular weight proteins slowest. More proteins of high and low molecular weights are transported into the dorsal root whereas more of those of medium molecular weight are transported towards the sciatic nerve.
D000818:Animals; D001370:Axonal Transport; D005727:Ganglia, Spinal / Q000032:analysis / Q000378:metabolism*; D008297:Male; D008970:Molecular Weight; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D013126:Spinal Nerve Roots / Q000032:analysis; D013127:Spinal Nerves / Q000032:analysis; D013997:Time Factors
D009419:Nerve Tissue Proteins