title: Reconstitution of Biological Molecular generators of electric current. Bacteriorhodopsin.
abstract: 1. Photoinduced generation of electric current by bacteriorhodopsin, incorporated into the planar phospholipid membrane, has been directly measured with conventional electrometer techniques. 2. Two methods for bacteriorhodopsin incorporation have been developed: (a) formation of planar membrane from a mixture of decane solution of phospholipids and of the fraction of violet fragments of the Halobacterium halobium membrane (bacteriorhodopsin sheets), and (b) adhesion of bacteriorhodopsin-containing reconstituted spherical membranes (proteoliposomes) to the planar membrane in the presence of Ca2+ or some other cations. In both cases, illumination was found to induce electric current generation directed across the planar membrane, an effect which was measured by macroelectrodes immersed into electrolyte solutions on both sides of the membrane. 3. The maximal values of the transmembrane electric potential were of about 150 mV at a current of about 10(-11) A. The electromotive force measured by means of counterbalancing the photoeffect by an external battery, was found to reach the value of 300 mV. 4. The action spectrum of the photoeffect coincides with the bacteriorhodopsin absorption spectrum (maximum about 570 nm). 5. Both components of the electrochemical potential of H+ ions (electric potential and delta pH) across the planar membrane affect the bacteriorhodopsin photoelectric response in a fashion which could be expected if bacteriorhodopsin were a light-dependent electrogenic proton pump. 6. La3+ ions were shown to inhibit operation of those bacteriorhodopsin which pump out H+ ions from the La3+-containing compartment. 7. The photoeffect, mediated by proteoliposomes associated with thick planar membrane, is decreased by gramicidin A at concentrations which do not influence the planar membrane resistance in the light. On the contrary, a protonophorous uncoupler, trichlorocarbonylcyanidephenylhydrazone, decreases the photoeffect only if it is added at a concentration lowering the light resistance. The dark resistance is shown to be higher than the light one, and decreases to the light level by gramicidin. 8. A simple equivalent electric scheme consistent with the above results has been proposed.
mesh_terms: D001436:Bacteriorhodopsins / Q000528:radiation effects; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone; D002338:Carotenoids / Q000528:radiation effects; D004492:Edetic Acid; D004553:Electric Conductivity; D004558:Electric Stimulation; D006096:Gramicidin; D006217:Halobacterium; D006863:Hydrogen-Ion Concentration; D007811:Lanthanum; D037102:Lectins; D008027:Light; D008081:Liposomes; D008567:Membranes, Artificial; D010777:Photochemistry; D011510:Proteolipids
chemical_list: D037102:Lectins; D008081:Liposomes; D008567:Membranes, Artificial; D011510:Proteolipids; D006096:Gramicidin; D002338:Carotenoids; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone; D001436:Bacteriorhodopsins; D007811:Lanthanum; D004492:Edetic Acid