Polystyrene tube immunoradiometric assay for human alpha1-fetoprotein, and its use for mass screening.
We describe a two-site immunoradiometric assay for human alpha1-fetoprotein, with use of antibody-coated polystyrene tubes as solid phase. The sensitivity, precision, and simplicity of this system make it eminently suitable for mass-screening purposes. We currently use it for neonatal detection of hereditary tyrosinemia in the Province of Quebec; measurements are made on blood spotted and dried on paper. This system could be well suited for other mass surveys, such as prenatal screenings for fetal abnormalities.
D000592:Amino Acid Metabolism, Inborn Errors / Q000097:blood; D005260:Female; D005312:Fetal Blood / Q000032:analysis; D006801:Humans; D005779:Immunodiffusion / Q000379:methods; D007223:Infant; D007231:Infant, Newborn; D008403:Mass Screening; D011247:Pregnancy; D011863:Radioimmunoassay / Q000379:methods; D014443:Tyrosine / Q000378:metabolism; D000509:alpha-Fetoproteins / Q000032:analysis*
D000509:alpha-Fetoproteins; D014443:Tyrosine