title: Terminal riboadenylate transferase from Escherichia coli. Characterization and application.
abstract: Catalytic properties of terminal riboadenylate transferase from Escherichia coli and the products of the enzymic reaction were investigated. The kinetic analysis revealed that the reaction obeys the sequential ordered bi-bi mechanism. The application of conditions elaborated in this study resulted in the synthesis of products of defined size and efficient primer utilization. The tRNA(rA)n obtained was a good template for the synthesis of complementary DNA with reverse transcriptase.
mesh_terms: D000249:Adenosine Monophosphate / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology; D007700:Kinetics; D009713:Nucleotidyltransferases / Q000378:metabolism*; D010441:Peptide Chain Elongation, Translational; D010442:Peptide Chain Initiation, Translational; D011062:Polynucleotide Adenylyltransferase / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism; D013696:Temperature; D013698:Templates, Genetic
chemical_list: D000249:Adenosine Monophosphate; D012343:RNA, Transfer; D009713:Nucleotidyltransferases; D011062:Polynucleotide Adenylyltransferase; D012194:RNA-Directed DNA Polymerase