Diurnal variations in endogenous RNA polymerase activity and amounts of nuclear non-histone protein, DNA and cytoplasmic protein in rat liver.
From rats fed ad libitum and kept under a 12 + 12 h light/dark regimen, the DNA dependent RNA polymerase activity of liver cell nuclei was determined avery four hours. From identical rats, nuclear non-histone protein and DNA, and cytoplasmic protein was determined by Feulgen-Naphthol Yellow S cytophotometry of isolated liver cells. The minimum: maximum ratio of the RNA polymerase activity is 0.77; the min:max ratio of nuclear non-histone protein is 0.84. These two parameters have identical time courses with a gradual decline during the light period and a sharp rise after the onset of the dark period. The variations in nuclear DNA content, estimated as the amount of Feulgen stain bound, closely parallel those of the RNA polymerase activity and nuclear non-histone protein content (min:max = 0.96). The amount of cytoplasmic protein per cell also varies throughout the day, but its time curve lags behind those of nuclear non-histone content and RNA polymerase activity. These results are consistent with the concept of nuclear non-histone proteins as de-repressors of the DNA template in differentiated, non-proliferating cells, and support the validity of using Feulgen-Naphthol Yellow S cytophotometry of nuclear non-histone proteins as an estimate of gene expression in such cells.
D000818:Animals; D001391:Azo Compounds; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis*; D002940:Circadian Rhythm; D004247:DNA / Q000032:analysis*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D005260:Female; D005557:Formaldehyde; D006651:Histocytochemistry; D006868:Hydrolysis; D008099:Liver / Q000032:analysis* / Q000201:enzymology; D051381:Rats; D013053:Spectrophotometry; D013194:Staining and Labeling
D001391:Azo Compounds; D002868:Chromosomal Proteins, Non-Histone; D005557:Formaldehyde; D004247:DNA; D012321:DNA-Directed RNA Polymerases