[Experiments on the biosynthesis of UDP-Glucuronyltransferase and UDP-Glucosyltransferase under the influence of eucalyptol (author's transl)].
To elucidate the reaction mechanism of the increased elimination rate of bilirubin in the blood of newborns under the influence of Eucalyptol, we performed a study on young mice. The following results were obtained: UDPGA-T activity is increased 33%, if measured with 4-MU as substrate after a single injection (i.p.) of Eucalyptol (0.3 mg/g body weight), with bilirubin as substrate the increase is 112%. UDPG-T activity increases 29%. The enhancement of enzyme activities can be inhibited by Actinomycin D (1 MUg/g body weight) 30% (bilirubin), 48% (4-MU) respectively in the case of UDPGA-T. Under cycloheximide (2 mug/g body weight) the increase of the activity of UDPGA-T is suppressed by 59% (bilirubin) or 99% (4-MU) as compared to the corresponding controls. No significant difference in the activity of UDPG-T by actinomycin or cycloheximide can be observed between the controls and the Eucalyptol-treated group. We conclude that the increase of enzyme activity of UDPGA-T is mainly due to enzyme induction, while in the case of UDPG-T activation of enzyme molecules might be the underlying mechanism. The effect of Eucalyptol on the bilirubin level in the blood of newborns is comparable to the effect of phenobarbital.
D000818:Animals; D001663:Bilirubin / Q000378:metabolism*; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D004789:Enzyme Activation; D004790:Enzyme Induction; D005052:Eucalyptus; D005964:Glucosyltransferases / Q000096:biosynthesis*; D006923:Hymecromone / Q000378:metabolism; D051379:Mice; D010430:Pentosyltransferases / Q000096:biosynthesis*; D010634:Phenobarbital / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal
D010936:Plant Extracts; D003609:Dactinomycin; D006923:Hymecromone; D003513:Cycloheximide; D005964:Glucosyltransferases; D010430:Pentosyltransferases; D001663:Bilirubin; D010634:Phenobarbital
Untersuchungen zum Wirkungsmechanismus von Eucalyptol auf die Biosynthese der UDP-Glucuronyltransferase und UDP-Glucosyltransferase