Relationship between beta2-microglobulin and cell-surface alloantigens of the mouse.
Molecular relationships between beta2m and other cell surface antigens (H-2, Tla, Ia, and Thy-1) were studied with the double immunofluorescence method. Cells were incubated with an antiserum against one antigen capped, and then tested with an antiserum against a second antigen. Capping of beta2m on thymocytes led to simultaneous capping of H-2 and Tla but not Thy-1 antigens; capping of H-2 and TIa (but not Thy-1) antigens resulted in capping of all beta2m detectable by the immunofluorescence method. Similarly, capping of beta2m on B or T lymphocytes resulted in capping of H-2 and vice versa. Ia antigens on B lymphocytes were not capped after the redistribution of beta2m. We conclude from these data that, in the cell membrane of thymocytes, virtually all the beta2m molecules are associated with H-2 and Tla, but not with Thy-1, and that on the cell surface of T or B lymphocytes, virtually all beta2m  is associated with H-2 but not with Ia. We found no evidence of any significant free beta2m on either thymocytes or splenocytes.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D001609:Beta-Globulins / Q000276:immunology*; D002462:Cell Membrane / Q000276:immunology*; D003429:Cross Reactions; D005260:Female; D005455:Fluorescent Antibody Technique; D006649:Histocompatibility Antigens; D007519:Isoantigens; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013601:T-Lymphocytes / Q000276:immunology; D001613:beta 2-Microglobulin / Q000276:immunology*
D001609:Beta-Globulins; D006649:Histocompatibility Antigens; D007519:Isoantigens; D001613:beta 2-Microglobulin