A cross-reaction between beta2-microglobulin and kappa-light chains.
Certain antisera to immunoglobulins containing kappa-chains show the presence of antibodies that cross-react with beta2-microglobulin. This was most apparent with an antiserum made to highly purified F(ab) fragments of Fr II gamma-globulin. These cross-reactive antibodies caused positive fluorescence and cytotoxicity reactions with a variety of cell types including T cells. These reactions were completely removed by absorption with highly purified kappa-chains but not with lambda-chains or lambda immunoglobulins. beta2-microglobulin preparations also absorbed or inhibited the special cellular reactivities. Evidence was obtained that HLA-bound beta2-microglobulin was more efficient in this respect. The possibility is discussed that similar cross-reactive antibodies may have been involved in some previous studies of inhibition of T cell function by immunoglobulin antisera.
D000042:Absorption; D000906:Antibodies; D001402:B-Lymphocytes / Q000276:immunology; D001609:Beta-Globulins / Q000276:immunology*; D002460:Cell Line; D003429:Cross Reactions; D005455:Fluorescent Antibody Technique; D006801:Humans; D007147:Immunoglobulin Light Chains; D007145:Immunoglobulin kappa-Chains; D009000:Monocytes / Q000276:immunology; D009194:Myeloma Proteins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology; D001613:beta 2-Microglobulin / Q000276:immunology*
D000906:Antibodies; D001609:Beta-Globulins; D007147:Immunoglobulin Light Chains; D007145:Immunoglobulin kappa-Chains; D009194:Myeloma Proteins; D001613:beta 2-Microglobulin