[Peroxidases of high molecular weight identified as the membrane peroxidases in lentils].
Peroxidases extracted from lentil roots are separated in two peaks by gel chromatography on Sephadex G-100 or on Bio Gel A-5 M. On both resins, the first peak of extremely large molecular weight is demonstrated to be an association of some peroxidases with microsomes. These enzymes can be detached from membranes by NaCl. Starch gel electrophoresis shows that isoperoxidases associated electrostatically to microsomes are basic peroxidases apparently not different from those of the soluble fraction.
D002462:Cell Membrane / Q000201:enzymology / Q000648:ultrastructure; D007527:Isoenzymes / Q000302:isolation & purification; D008854:Microscopy, Electron; D008861:Microsomes / Q000201:enzymology; D008970:Molecular Weight; D010544:Peroxidases / Q000302:isolation & purification; D010944:Plants / Q000201:enzymology*
D007527:Isoenzymes; D010544:Peroxidases
Peroxydases de haut poids molĕculaire identifiées à des peroxydases membranaires chez la lentille.