Effects of streptovaricins and their degradation products on RNA-directed DNA polymerase of Rauscher leukemia virus.
The activities of streptovaricin complexes, streptovaricins, streptovals, and streptovarinic degradation products were elevated against RNA-directed DNA polymerases of Rauscher leukemia virus, DNA-dependent DNA polymerase of bacterial and mammalian cells, and DNA-dependent RNA polymerases of mammalian origin. The activities of streptovaricins were also listed for comparison purposes. The effects of streptovaricin complexes on viral DNA polymerases varied significantly from lot to lot, and streptovaricin complex lot 7 was the most active. All the streptovals and streptovaricin degradation products except varicinal A showed a marked improvement (twofold to tenfold) in activity against the viral enzyme over the parent streptovaricins. None of these compounds, however, displayed any significant effect on either the DNA polymerase of L1210 leukemia cells and Escherichia coli or the RNA polymerase of isolated nuclei of mouse liver. As a result of tests in these systems, some specific inhibitors of RNA-directed DNA polymerases of Rauscher leukemia virus were selected.
D055598:Chemical Phenomena; D002621:Chemistry; D012321:DNA-Directed RNA Polymerases / Q000037:antagonists & inhibitors; D003609:Dactinomycin / Q000494:pharmacology; D066298:In Vitro Techniques; D009369:Neoplasms / Q000201:enzymology; D019384:Nucleic Acid Synthesis Inhibitors; D011925:Rauscher Virus / Q000201:enzymology*; D018894:Reverse Transcriptase Inhibitors; D013310:Streptovaricin / Q000378:metabolism / Q000494:pharmacology*; D013329:Structure-Activity Relationship
D019384:Nucleic Acid Synthesis Inhibitors; D018894:Reverse Transcriptase Inhibitors; D013310:Streptovaricin; D003609:Dactinomycin; D012321:DNA-Directed RNA Polymerases