A rapid alpha-fetoprotein radioimmunoassay.
A simple, reliable and inexpensive alpha-fetoprotein radioimmunoassay is described, in which polyethylene-glycol (PEG) is used as a precipitating agent of antibody-bound AFP. The concentrations of AFP found in amniotic fluid, newborn and maternal sera are in agreement with those reported by others. This assay fulfills the requirements of a screening test for fetal abnormalities and other clinical applications.
D000653:Amniotic Fluid / Q000276:immunology; D000818:Animals; D000917:Antibody Formation; D011232:Chemical Precipitation; D005260:Female; D005315:Fetal Diseases / Q000276:immunology; D006801:Humans; D007106:Immune Sera; D011092:Polyethylene Glycols; D011247:Pregnancy; D011296:Prenatal Diagnosis; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods*; D000509:alpha-Fetoproteins / Q000302:isolation & purification*
D007106:Immune Sera; D000509:alpha-Fetoproteins; D011092:Polyethylene Glycols