The use of hapten-polysaccharide conjugates for the induction of B-cell tolerance involving IgE responses. III. Specific tolerance induced by sulphonamide-substituted levan in the guinea pig.
Inbred strain 2 and (strain 2 x strain 13)F1 guinea pigs were made allergic by intraperitoneal injections of sulphonamide-chicken gamma-globulin (CGG) conjugates. Two sulphonamides were used: 4-sulphanilamide benzoic acid (4-SABA) and sulphamethoxazole (SMX). 4-SABA was coupled to CGG through its carboxylic group and SMX was coupled following diazotization of its sulphanilamide group. The anti-4-SABA and anti-SMX reaginic antibodies formed did not show any cross-reactivity with each other. Injection of 4-SABA coupled to native levan effectively suppressed the allergic responses of these guinea pigs when given either prior to or after immunization with 4-SABA-CGG. This treatment is specific as it did not affect anti-SMX or anti-CGG reaginic responses. Guinea pigs seem more sensitive to the regime used for tolerance induction than correspondingly sensitized mice in that less tolerogen is required on a body weight basis.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D002645:Chickens; D003429:Cross Reactions; D005630:Fructans / Q000276:immunology; D006168:Guinea Pigs; D006241:Haptens / Q000276:immunology*; D007108:Immune Tolerance; D007073:Immunoglobulin E / Q000096:biosynthesis*; D011134:Polysaccharides / Q000276:immunology*; D013449:Sulfonamides / Q000276:immunology; D005719:gamma-Globulins / Q000276:immunology
D005630:Fructans; D006241:Haptens; D011134:Polysaccharides; D013449:Sulfonamides; D005719:gamma-Globulins; D007073:Immunoglobulin E