[Histochemical demonstration of glycogen phosphorylase (E.C. 2.4.11) through the use of semipermeable membranes (author's transl)].
A method is described for the histochemical demonstration of phosphorylase in unfixed cryostat sections of rat liver using semipermeable membranes and a gel medium. In comparison to the conventional methods this procedure has the following advantages: 1. Staining of sections through a semipermeable membrane prevents diffusion of cellular glycogen and guarantees optimal localisation of phosphorylase activity. 2. Since diffusion is effectively prevented by the membrane the total activity of this highly soluble enzyme can be demonstrated. 3. Tissue and cell structures are well preserved.
D000818:Animals; D006651:Histocytochemistry / Q000379:methods*; D008099:Liver / Q000032:analysis / Q000201:enzymology*; D008112:Liver Glycogen / Q000032:analysis; D008297:Male; D008567:Membranes, Artificial; D006005:Phosphorylases / Q000032:analysis*; D051381:Rats; D013194:Staining and Labeling
D008112:Liver Glycogen; D008567:Membranes, Artificial; D006005:Phosphorylases
Histochemische Darstellung der Glykogenphosphorylase (E.C. 2.4.11) mit Hilfe semipermeabler Membranen.