[Mechanism of metabolic and ionic germination of "Bacillus licheniformis" spores treated with hydrogen peroxide (author's transl)].
Spores of Bacillus licheniformis 109-2A0 lost their refractility and absorbancy at 640 nm in the presence of metabolizable molecules (L-alanine). The same occurred with spores treated with 4.4 mol/1 hydrogen peroxide, pH 2.0, at 65 degrees C, even after 5 min of treatment. In addition, these transformations could be promoted after 2 min of treatment by inorganic ions (KI). This possibility occurs following a kinetics of activation. Thermodynamic parameters showed this activation to be combined with a molecular re-organization. Loss of refractility or absorbancy, induced by L-ala or KI, was inhibited by inhibitors of membrane functions or of L-alanine dehydrogenase, enzyme of which a noticeable activity was demonstrated in treated spores. Only 10% of spore calcium leaked during the treatment. Therefore loss of refractility or absorbancy caused by molecules metabolizable or not seemed to correspond to a physiological germination. The first even of the metabolic, as well as or the ionic germination could well be a modification of the spore membrane proton-motive force.
D000409:Alanine / Q000494:pharmacology; D000594:Amino Acid Oxidoreductases / Q000378:metabolism; D001407:Bacillus / Q000187:drug effects / Q000502:physiology*; D002118:Calcium / Q000494:pharmacology; D001728:Dicumarol / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D008773:Methylphenazonium Methosulfate / Q000494:pharmacology; D010416:Pentachlorophenol / Q000494:pharmacology; D010710:Phosphates / Q000494:pharmacology; D011193:Potassium Iodide / Q000494:pharmacology; D013171:Spores, Bacterial / Q000187:drug effects / Q000201:enzymology; D013696:Temperature; D013816:Thermodynamics; D062367:ortho-Aminobenzoates / Q000494:pharmacology
D010710:Phosphates; D062367:ortho-Aminobenzoates; D011193:Potassium Iodide; D008773:Methylphenazonium Methosulfate; D001728:Dicumarol; D006861:Hydrogen Peroxide; D010416:Pentachlorophenol; D000594:Amino Acid Oxidoreductases; D000409:Alanine; D002118:Calcium
Mécanisme de la germination métabolique et de la germination ionique des spores de Bacillus licheniformis traitées par le peroxyde d'hydrogène.