Electron microscopic detection of phleomycin during different waves of replication in regenerating skeletal muscle of mice following staining with mercuric chloride.
Phleomycin has devastating effects on regeneration of skeletal muscle when applied during an early wave of replication in the pre-myotube (new fibre) period of the reaction. The effect cannot be explained from the contribution of dividing cells to myotubes. Phleomycin's effects on regeneration are much less severe when the challenge coincides with a later wave of pre-myotube proliferation, effects that can be explained from the contribution of such cells to new muscle. An attempt has been made, by means of electron microscopy, to explain how phleomycin distributes early versus late wave cells, using a mercury substitution stain to detect the antibiotic. Cells of the two periods showed conspicuous differences in the staining characteristics of their chromatin. Positive staining reactions outside the nucleus were confined mainly to ribosomes. Exceptions included materials in transit across nuclear and cytoplasmic membranes.
D000818:Animals; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D002455:Cell Division; D002843:Chromatin / Q000378:metabolism; D006651:Histocytochemistry; D008297:Male; D008628:Mercury; D051379:Mice; D009132:Muscles / Q000166:cytology / Q000378:metabolism* / Q000502:physiology; D010692:Phleomycins / Q000378:metabolism*; D012038:Regeneration; D013194:Staining and Labeling; D014945:Wound Healing
D000900:Anti-Bacterial Agents; D002843:Chromatin; D010692:Phleomycins; D008628:Mercury