Immunofluorescent detection of differentiation alloantigens (CA1) in the chicken.
Lymphoid cell surface alloantigens were detected by a triple-layer immunofluorescence technique. An antiserum raised against B locus-incompatible lymphoid cells also reacted with previously undefined differentiation antigens (CA1) which segregated between B locus homozygous (B14/B14) chickens of the same strain as the antiserum donor. CA1-positive chickens reacted with the antiserum by agglutination of red cells and staining of all peripheral lymphocytes and thymocytes but not bursal cells. Cross-absorption experiments have demonstrated that antigens expressed by red cells, T cells (both thymic and peripheral) and B cells (peripheral only) respectively, were of distinct specificity.
D000818:Animals; D000961:Antilymphocyte Serum / Q000494:pharmacology; D001402:B-Lymphocytes / Q000276:immunology; D002454:Cell Differentiation; D002462:Cell Membrane / Q000276:immunology; D002645:Chickens; D000939:Epitopes; D004912:Erythrocytes / Q000276:immunology; D005455:Fluorescent Antibody Technique; D005796:Genes; D006386:Hemagglutination Tests; D006649:Histocompatibility Antigens; D007519:Isoantigens; D008214:Lymphocytes / Q000276:immunology*; D010641:Phenotype; D013045:Species Specificity; D013601:T-Lymphocytes / Q000276:immunology
D000961:Antilymphocyte Serum; D000939:Epitopes; D006649:Histocompatibility Antigens; D007519:Isoantigens