Effects of anti-guinea-pig beta 2-microgloblin antibodies on lymphocyte transformation induced by specific antigens or mitogens.
The effects of a goat anti-guinea-pig beta 2-microglobulin antiserum (a beta 2m) on lymphocyte transformation, induced by specific antigens or mitogens, were studied. a beta 2m was found to exert inhibitory effects on antigen stimulation, with three different antigens (purified protein devivative, hen egg-white lysozyme, and ovalbumin), and on stimulation induced by the 'T-cell mitogens', concanavalin A and phytohaemagglutinin, and the 'mixed mitogen', pokeweed mitogen. Stimulation induced by the 'B-cell mitogens', dextran sulphate and bacterial lipopolysaccharide, did not seem to be inhibited to the same extent by a beta 2m. The inhibitory effects seemed specific, as they were not seen with a beta 2m that had been absorbed on a column with insolubilized purified guinea-pig beta 2m nor with normal goat serum. Time studies indicated that the inhibitions started already during the first day of culture.
D000818:Animals; D000941:Antigens; D001402:B-Lymphocytes / Q000276:immunology; D001609:Beta-Globulins / Q000276:immunology*; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D006041:Goats / Q000276:immunology; D006168:Guinea Pigs; D007106:Immune Sera / Q000494:pharmacology*; D008198:Lymph Nodes / Q000166:cytology; D008213:Lymphocyte Activation / Q000187:drug effects*; D010835:Phytohemagglutinins / Q000494:pharmacology; D011043:Pokeweed Mitogens / Q000494:pharmacology; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology; D001613:beta 2-Microglobulin / Q000276:immunology*
D000941:Antigens; D001609:Beta-Globulins; D007106:Immune Sera; D010835:Phytohemagglutinins; D011043:Pokeweed Mitogens; D001613:beta 2-Microglobulin; D003208:Concanavalin A; D004247:DNA