[Effect of substances that alter the level of intracellular cAMP on the reaction of human lymphocytes blast transformation].
Effects of cholera toxin, cholera anatoxin, theophylline and dibutyryl cyclic AMP (db-cAMP) on the PHA-induced DNA synthesis have been studied. It was shown that toxin, db-cAMP and theophylline suppressed mitogen-induced 3H-TdR incorporation and blast-transformation in a dose-related manner. When both toxin and theophylline were given together to PHA-stimulated lymphocytes, the inhibitory effects of these two agents were additive. The inhibitory effects of db-cAMP and theophylline were additive too. This inhibitory effects could be due to the elevation of intracellular cAMP. Cholera anatoxin exerted an inhibitory action on the PHA-stimulated lymphocytes. The inhibitory effects on lymphocyte DNA synthesis by anatoxin and theophylline were not additive. These results suggest that the effects of cholera toxin are mediated by cyclic AMP, but effects of cholera anatoxin may be associated with some other mechanism.
D000942:Antigens, Bacterial / Q000032:analysis; D003994:Bucladesine / Q000276:immunology*; D002478:Cells, Cultured; D002772:Cholera Toxin / Q000276:immunology*; D000242:Cyclic AMP / Q000502:physiology*; D004247:DNA / Q000096:biosynthesis; D003864:Depression, Chemical; D004306:Dose-Response Relationship, Immunologic; D004357:Drug Synergism; D000939:Epitopes; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects*; D013806:Theophylline / Q000276:immunology*; D014121:Toxoids / Q000276:immunology*
D000942:Antigens, Bacterial; D000939:Epitopes; D014121:Toxoids; D003994:Bucladesine; D004247:DNA; D002772:Cholera Toxin; D013806:Theophylline; D000242:Cyclic AMP
Vliianie veshchestv, izmeniaiushchikh uroven' vnutrikletochnogo tsAMF, na reaktsiiu blasttransformatsii limfotsitov cheloveka.