Effects of adriamycin on the reverse transcriptase and the production of murine leukemia virus.
Adriamycin inhibited the endogenous RNA-, poly (A)-d(T)12-, and calf thymus DNA-catalyzed reaction of reverse transcriptase from AKR mouse murine leukemia virus (AKR-MLV). This inhibition was found at the reaction levels of endogenous RNA-directed and subsequent DNA-directed DNA synthesis. Although adriamycin and actinomycin D significantly reduced the growth of AKR mouse cells (K3b), the treatment with adriamycin could bot inhibit the AKR-MLV production in these cells. Actinomycin D inhibited AKR-MLV production completely in the same experimental condition. In adriamycin-resistant K3b/Am cells, which were isolated by intermittent treatment of K3b cells with adriamycin, persistence of AKR-MLV was demonstrated. K3b/Am cells showed some altered characteristics such as reduced growth rate and tumorigenicity.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003609:Dactinomycin / Q000494:pharmacology; D004317:Doxorubicin / Q000494:pharmacology*; D004351:Drug Resistance; D009052:Leukemia Virus, Murine / Q000187:drug effects* / Q000201:enzymology; D051379:Mice; D009374:Neoplasms, Experimental / Q000209:etiology; D018894:Reverse Transcriptase Inhibitors; D014779:Virus Replication / Q000187:drug effects
D018894:Reverse Transcriptase Inhibitors; D003609:Dactinomycin; D004317:Doxorubicin