Association of the H-Y male antigen with beta2-microglobulin on human lymphoid and differentiated mouse teratocarcinoma cell lines.
The expression of the H-Y antigen has been tested on several human lymphoid lines and mouse teratocarcinoma cell lines during differentiation. The human male lymphoid cell line Raji is a very useful target for studies of the H-Y antigen by lymphocytotoxicity test with rat anti-H-Y sera. With a few exceptions, all cells carrying the Y chromosome were H-Y positive. One of the exceptions is the human Daudi cell line which, besides lacking H-Y antigen, also lacks beta2-microglobulin. We have studied a possible association between the H-Y antigen, beta2-microglobulin, and HLA antigen with redistribution experiments. The results strongly suggest that H-Y antigen is not associated with HLA antigens but with beta2-microglobulin.
D000818:Animals; D001609:Beta-Globulins / Q000276:immunology*; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D002478:Cells, Cultured; D003601:Cytotoxicity Tests, Immunologic; D000939:Epitopes; D005260:Female; D006680:HLA Antigens; D006649:Histocompatibility Antigens; D006801:Humans; D007106:Immune Sera; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D051381:Rats; D012730:Sex Chromosomes; D013724:Teratoma / Q000276:immunology*; D014998:Y Chromosome; D001613:beta 2-Microglobulin / Q000276:immunology*
D001609:Beta-Globulins; D000939:Epitopes; D006680:HLA Antigens; D006649:Histocompatibility Antigens; D007106:Immune Sera; D001613:beta 2-Microglobulin