Quantitation of human granulocyte protease inhibitors in non-purulent bronchial lavage fluids.
The predominant inhibitors of granulocyte proteases in plasma (alpha1-antitrypsin, alpha1-antichymotrypsin, and alpha2-macroglobulin) were quantitated in unconcentrated bronchial lavage fluids obtained from non-infected individuals, together with the acid-stable low molecular weight inhibitor with activity against granulocyte elastolytic and chymotrypsin-like enzymes. This latter inhibitor accounted for about 90% of the total molar concentration of granulocyte protease inhibitors in the bronchial lavage fluids. The remaining 10% consisted mostly of alpha1-antitrypsin and alpha1-antichymotrypsin. About 85% of the bronchial inhibitor was in a free form with preserved enzyme reactivity. The remaining 15% of the immunoreactive bronchial inhibitor exhibited a molecular size indicating complexation with enzymes. The major portion of alpha1-antitrypsin and alpha1-antichymotrypsin showed electrophoretic mobilities and molecular sizes similar to the native proteins but had no enzyme reactivity.
D000328:Adult; D000368:Aged; D001826:Body Fluids / Q000201:enzymology*; D001980:Bronchi / Q000201:enzymology / Q000378:metabolism*; D002850:Chromatography, Gel; D002918:Chymotrypsin / Q000032:analysis; D005260:Female; D006098:Granulocytes / Q000201:enzymology*; D006801:Humans; D007106:Immune Sera; D007118:Immunoassay; D005779:Immunodiffusion; D007123:Immunoelectrophoresis, Two-Dimensional; D007962:Leukocytes / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D011480:Protease Inhibitors; D000515:alpha 1-Antitrypsin / Q000032:analysis; D000511:alpha-Macroglobulins / Q000032:analysis
D007106:Immune Sera; D011480:Protease Inhibitors; D000515:alpha 1-Antitrypsin; D000511:alpha-Macroglobulins; D002918:Chymotrypsin