Preparation of anti-alpha 2-macroglobulin using Canavalia lineata DC lectin for differentiating species-specificity of blood stains.
To differentiate species-specificity of blood stains, anti-alpha 2-macroglobulin was raised in rabbits against Canavalia lineata DC lectin-serum complex (LSC). Adsorption of anti-LSC with human lipoprotein resulted in antiserum specific for alpha 2-macroglobulin. It was confirmed by Ouchterlony test that the antiserum adsorbed successively with monkey serum or anti-LSC adsorbed directly with monkey serum reacted with only human serum but not with mammalian ones. Immunoelectrosyneresis and anti-LSC consumption test could identify species-specificity of blood stains kept for up to two years and for up to several years, respectively. It is indicated that anti-LSC is quite effective for differentiating species-specificity of blood stains.
D000327:Adsorption; D000818:Animals; D001801:Blood Stains; D011232:Chemical Precipitation; D005554:Forensic Medicine; D000882:Haplorhini; D006801:Humans; D007106:Immune Sera; D007122:Immunoelectrophoresis; D037102:Lectins / Q000276:immunology*; D008074:Lipoproteins / Q000276:immunology; D008722:Methods; D037121:Plant Lectins; D010944:Plants / Q000276:immunology; D011817:Rabbits; D013045:Species Specificity; D000511:alpha-Macroglobulins / Q000276:immunology*
D007106:Immune Sera; D037102:Lectins; D008074:Lipoproteins; D037121:Plant Lectins; D000511:alpha-Macroglobulins