Rapid purification of an RNA tumor virus and proteins by high-performance steric exclusion chromatography on porous glass bead columns.
High-performance steric exclusion chromatography on a 1250-A pore size polyethylene glycol-treated glass bead column was used to purify avian myeloblastosis virus and hamster melanoma virus from plasma protein and tissue culture media. The purified hamster melanoma virus was still infectious and the avian myeloblastosis virus-associated RNA-directed DNA polymerase showed a 1100-fold purification of the virus from one column treatment. Electron microscopy of the purified virus showed intact particles, with surface projections evident. The time required for column purification of the virus was 5 min.
D000469:Alkaline Phosphatase / Q000302:isolation & purification; D009189:Avian Myeloblastosis Virus / Q000201:enzymology / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D004926:Escherichia coli / Q000201:enzymology; D008722:Methods; D009858:Oncogenic Viruses / Q000201:enzymology / Q000302:isolation & purification*; D012194:RNA-Directed DNA Polymerase / Q000302:isolation & purification*
D012194:RNA-Directed DNA Polymerase; D000469:Alkaline Phosphatase