Biochemical and immunological characterization of the major envelope glycoprotein of bovine leukemia virus.
The major envelope glycoprotein of bovine leukemia virus was isolated by lectin-bound Sepharose and DEAE-cellulose column chromatography. This protein was shown to have a molecular weight of about 41,000 and to lack detectable immunological cross-reactivity with glycoproteins of other oncornaviruses. Sera obtained from 100% of cattle examined with clinically diagnosed lymphosarcoma contained high-titered antibody to 125I-labeled bovine leukemia virus glycoprotein, whereas sera from animals in a disease-free herd were antibody negative.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology; D000939:Epitopes; D006023:Glycoproteins / Q000032:analysis* / Q000276:immunology; D001909:Leukemia Virus, Bovine / Q000032:analysis* / Q000276:immunology; D008228:Lymphoma, Non-Hodgkin / Q000276:immunology / Q000662:veterinary; D008970:Molecular Weight; D012190:Retroviridae / Q000032:analysis*; D014764:Viral Proteins / Q000032:analysis* / Q000276:immunology
D000914:Antibodies, Viral; D000939:Epitopes; D006023:Glycoproteins; D014764:Viral Proteins