title: Axonal transport of nucleosides, nucleotides and 4S RNA in the neonatal rat visual system.
abstract: The axonal migration of RNA, the nucleoside uridine and its nucleotide derivates (NS/NT) were compared in neonatal and young adult rat optic axons. Tritiated uridine was injected into right eyes of developing (1- or 4-day-old) and young adult (40-day-old) rats which were sacrificed at times after injection ranging from 6 h to 20 days. Right and left lateral geniculates were removed and assayed for trichloroacetic acid soluble (NS/NT) and RNA radioactivity. Left minus right geniculate (L-RLG) radioactivity was used as an index of axonally migrating radioactivity. Results showed that uridine and its phosphorylated derivatives were transported along both neonatal and young adult rat optic axons. Greater than 90% of right geniculate (blood-borne) TCA soluble radioactivity was metabolized to volatile substances (probably 3H2O) by three days after injection, leaving approximately 3% of the neonatal and approximately 10% of the adult activity as [3H]NS/NT. In left geniculate fractions (containing transported material) approximately 15% and 40% of total TCA soluble radioactivity was present as [3H]NS/NT in neonates and adults, respectively. Thus, axonal NS/NT appears to be relatively protected from degradation when compared with blood-borne NS/NT. The amount of L-RLG [3H]RNA in the neonates was 10 times higher than in young adults. Peaks of neonatal [3H]RNA occurred at 5 and 10 days after birth, whether injections were made at 1 or 4 days of age indicating that this [3H]RNA may be linked to developmental events. Gel electrophoretic analysis of neonatal geniculate RNA indicated that a small portion of the [3H]RNA in the first peak represented axonally transported 4S RNA. The remainder of the L-RLG [3H]RNA in the neonates was probably due to a rapid and efficient incorporation of axonally transported [3H]NS/NT into extraaxonal geniculate RNA. In contrast, little or no axonal RNA transport could be demonstrated in the young adults.
mesh_terms: D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D001370:Axonal Transport; D004292:Dominance, Cerebral / Q000502:physiology; D004591:Electrophoresis, Polyacrylamide Gel; D005829:Geniculate Bodies / Q000378:metabolism; D008297:Male; D009705:Nucleosides / Q000378:metabolism*; D009711:Nucleotides / Q000378:metabolism*; D009900:Optic Nerve / Q000378:metabolism*; D012313:RNA / Q000378:metabolism*; D051381:Rats; D014529:Uridine / Q000378:metabolism; D014530:Uridine Diphosphate / Q000378:metabolism; D014542:Uridine Monophosphate / Q000378:metabolism; D014544:Uridine Triphosphate / Q000378:metabolism; D014795:Visual Pathways / Q000378:metabolism
chemical_list: D009705:Nucleosides; D009711:Nucleotides; D014530:Uridine Diphosphate; D012313:RNA; D014542:Uridine Monophosphate; D014544:Uridine Triphosphate; D014529:Uridine