title: Cross-linking studies on the conformation and dimerization of myelin basic protein in solution.
abstract: Myelin basic protein was isolated from both cat and bovine central nervous system. Cat and bovine myelin basic protein, which are shown to be similar by tryptic mapping, exhibit identical behavior when cross-linked with the bifunctional reagent difluorodinitrobenzene. Myelin basic protein is cross-linked into only a dimer under certain conditions in the presence of sodium dodecyl sulfate. In contrast, many oligomers are formed when myelin basic protein is cross-linked in the absence of detergent. The formation of cross-linked dimers in the absence of other oligomer formation suggests that the protein is at least partly dimeric in the presence of sodium dodecyl sulfate. The conformation of them myelin basic protein monomer in sodium dodecyl sulfate was also studied. N-Bromosuccinimide and cyanogen bromide cleavage reactions were used to demonstrate that difluorodinitrobenzene had introduced intramolecular cross-links between the two peptides resulting from each of the cleavage ractions. However, these types of intramolecular cross-links cannot be detected under conditions in which only dimers have formed. Some of the lysine residues which are modified by difluorodinitrobenzene were identified by tryptic mapping. In several respects, the conformation of myelin basic protein in a sodium dodecyl sulfate solution appears to be similar to the conformation of the protein in the membrane.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002415:Cats; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D004139:Dinitrofluorobenzene / Q000031:analogs & derivatives; D046911:Macromolecular Substances; D004676:Myelin Basic Protein; D009186:Myelin Sheath / Q000648:ultrastructure*; D010446:Peptide Fragments / Q000032:analysis; D011487:Protein Conformation; D013116:Spinal Cord
chemical_list: D000596:Amino Acids; D046911:Macromolecular Substances; D004676:Myelin Basic Protein; D010446:Peptide Fragments; D004139:Dinitrofluorobenzene