Demonstration of thymus-leukemia (TL) antigens on mitochondria of lymphoid cells by immunoelectron microscopy.
The presence of thymus-leukemia (TL) antigens on the surfaces of mitochondria isolated from TL+ cells, e.g., RADA 1 leukemia cells, and TL+ normal thymocytes, has been directly demonstrated by immunoelectron microscopy. Reagents used were TL alloantiserum and a hemocyanin conjugate of rabbit antibody to mouse IgG. No hemocyanin labeling was observed on mitochondria obtained from TL- cells, e.g., thymocytes of TL- strains, splenocytes of either TL+ or TL- strains, and TL- leukemia cells. The concurrence of TL antigens on the plasma and mitochondrial membranes was shown by the ability of intact cells to absorb all reactivity toward mitochondria.
D000818:Animals; D000941:Antigens / Q000032:analysis*; D000951:Antigens, Neoplasm / Q000032:analysis*; D002458:Cell Fractionation; D006433:Hemocyanins; D007158:Immunologic Techniques; D007942:Leukemia, Experimental / Q000276:immunology*; D008722:Methods; D051379:Mice; D008815:Mice, Inbred Strains; D008854:Microscopy, Electron; D008928:Mitochondria / Q000276:immunology*; D013194:Staining and Labeling; D013950:Thymus Gland / Q000276:immunology*
D000941:Antigens; D000951:Antigens, Neoplasm; D006433:Hemocyanins