In vitro tolerance induction of neonatal murine B cells as a probe for the study of B-cell diversification.
The susceptibility to in vitro tolerance induction has been implicated as a characteristic of B cells early in their development, since DNP-reactive B cells are tolerizable only during the first days after birth, and 25% of adult bone marrow cells are tolerizable. In the present study, a modification of the in vitro splenic focus technique was utilized to determine if PC-specific B cells, by virtue of their late expression (approximately 1 wk post-parturition), also display susceptibility to tolerance induction. The results demonstrate that at 7-10 days after birth, when over 90% of the DNP-specific splenic B cells are resistant to tolerance induction, the majority of PC-specific B cells are tolerizable. These results re-emphasize tolerance susceptibility as a characteristic of developing clones, confirm the late acquisition of PC-specific B cells, and support the contention that the acquisition of the specificity repertoire is a highly ordered, specifically predetermined process which is independent of antigen-driven events.
D000818:Animals; D000831:Animals, Newborn / Q000276:immunology*; D000918:Antibody Specificity; D000941:Antigens; D001402:B-Lymphocytes / Q000276:immunology*; D002454:Cell Differentiation; D004136:Dinitrobenzenes / Q000276:immunology; D006433:Hemocyanins / Q000276:immunology; D007108:Immune Tolerance; D007116:Immunization, Passive; D051379:Mice; D008807:Mice, Inbred BALB C; D010767:Phosphorylcholine / Q000276:immunology; D011863:Radioimmunoassay; D005719:gamma-Globulins
D000941:Antigens; D004136:Dinitrobenzenes; D005719:gamma-Globulins; D010767:Phosphorylcholine; D006433:Hemocyanins