Induction and properties of DNP-specific T cells in mice sensitized to DNP-coupled mycobacterium.
Splenic T lymphocytes from mice sensitized to 100 microgram of DNP-coupled mycobacterium (DNP-Tbc) showed in vitro proliferative response against DNP- or TNP-conjugated protein antigens. The increased uptake of 3H-thymidine induced by DNP-HSA was partially inhibited by the addition of 10(-4)M DNP-EACA. DNP-AECM-Ficoll did not induce any significant proliferative responses in DNP-Tbc-primed T cell population. However, priming with DNP-Tbc augmented anti-DNP IgG antibody response induced with DNP-Ficoll. The augmentation of IgG response was not due to the presence of DNP-primed B cells or anti-DNP antibody. The results showed that the priming with DNP-Tbc induced DNP-reactive T helper cells which could be triggered with DNP-Ficoll. The possible role of mycobacterium in the induction of hapten-specific T helper cells is discussed.
D015119:Aminocaproic Acid; D000818:Animals; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology; D004140:Dinitrophenols / Q000276:immunology*; D000939:Epitopes; D006241:Haptens; D007074:Immunoglobulin G / Q000096:biosynthesis; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D009161:Mycobacterium; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism
D004140:Dinitrophenols; D000939:Epitopes; D006241:Haptens; D007074:Immunoglobulin G; D015119:Aminocaproic Acid; D013936:Thymidine