Determination of some L-3,4-dihydroxyphenylalanine and dopamine metabolites in urine by means of mass fragmentography.
We describe a mass-fragmentographic method for determination in urine of the following metabolites of L-3,4-dihydroxyphenylalanine and dopamine: vanillactic acid, 3,4-dihydroxyphenylacetic acid, 3-methoxy-4-hydroxyphenylethanol, and 3,4-dihydroxyphenylethanol. Deuterated analogs were used as internal standards. The method is fast, reproducible, sensitive, and selective, and does not require the use of time-consuming clean-up procedures. Normal excretion values in terms of creatinine, expressed as a function of age, as well as values obtained for patients with neurogenic tumors, a patient during therapy with L-3,4-dihydroxyphenylalanine, and a patient receiving dopamine are presented and discussed.
D015102:3,4-Dihydroxyphenylacetic Acid / Q000652:urine; D015098:3-Methoxy-4-hydroxyphenylethanol / Q000652:urine; D000328:Adult; D000375:Aging; D002648:Child; D002675:Child, Preschool; D004295:Dihydroxyphenylalanine / Q000652:urine*; D004298:Dopamine / Q000627:therapeutic use / Q000652:urine*; D005421:Flavoring Agents / Q000031:analogs & derivatives / Q000652:urine; D008401:Gas Chromatography-Mass Spectrometry; D006719:Homovanillic Acid / Q000652:urine / Q000031:analogs & derivatives; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007773:Lactates / Q000652:urine; D007980:Levodopa / Q000627:therapeutic use; D008875:Middle Aged; D009447:Neuroblastoma / Q000652:urine; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives / Q000652:urine
D005421:Flavoring Agents; D007773:Lactates; D015102:3,4-Dihydroxyphenylacetic Acid; C005975:3,4-dihydroxyphenylethanol; D015098:3-Methoxy-4-hydroxyphenylethanol; C003753:vanillactic acid; D007980:Levodopa; D004295:Dihydroxyphenylalanine; D010626:Phenylethyl Alcohol; D004298:Dopamine; D006719:Homovanillic Acid