title: Observations on the orthograde and retrograde transport of horseradish peroxidase in the cat.
abstract: Horseradish peroxidase (HRP) was applied to the precruciate cortex of the cat. The peroxidase was either applied directly to the surface or injected by glass micropipettes in single or multiple injections. Retrograde as well orthograde transport was observed by light and electron microscopy in different areas: in the thalamus: the nucleus ventralis lateralis and the nucleus centrum medianum, in the red nucleus and in the spinal cord. The distribution of HRP within the nervous elements of the nuclei: axon terminals and/or perikarya and dendrites, was observed by light and electron microscopy. HRP was present in dendrites and perikarya, indicating retrograde transport, in experiments where damage to the injected nervous tissue could be suspected. However HRP appeared restricted to the nerve endings, indicating orthograde transport, when the damage seemed to be minimal. This effect of injury on retrograde transport of HRP was further confirmed by experiments using bilateral HRP injections and unilateral lesions of the spinal cord to label rubro-spinal cells. Thus retrograde transport of HRP could be induced by lesion in systems where it does not occur normally. The normal intra-axonal transport of HRP appears to be from the perikaryon to the nerve endings. This orthograde transport can be seen under the light microscope and can be confused with faint retrograde labeling.
mesh_terms: D000818:Animals; D001370:Axonal Transport; D001931:Brain Mapping; D002415:Cats; D003712:Dendrites / Q000378:metabolism / Q000648:ultrastructure; D006735:Horseradish Peroxidase / Q000378:metabolism*; D009411:Nerve Endings / Q000378:metabolism; D009434:Neural Pathways / Q000033:anatomy & histology / Q000378:metabolism; D009474:Neurons / Q000378:metabolism / Q000648:ultrastructure; D010544:Peroxidases / Q000378:metabolism*; D012012:Red Nucleus / Q000033:anatomy & histology / Q000378:metabolism; D013116:Spinal Cord / Q000033:anatomy & histology / Q000378:metabolism; D013787:Thalamic Nuclei / Q000033:anatomy & histology / Q000378:metabolism
chemical_list: D006735:Horseradish Peroxidase; D010544:Peroxidases