Methods for removing uranyl acetate precipitate from ultrathin sections.
Precipitate resulting from en bloc staining with uranyl acetate was removed by treating sections with 15% oxalic acid in 50% methanol for 30 minutes at 40 C. Precipitate resulting from poststaining sections with hot uranyl acetate was removed by rinsing sections in 0.25-0.50% aqueous oxalic acid for 10-15 seconds at room temperature. Rinsing sections for longer than 30 seconds removed uranyl precipitate and also destained the sections. These procedures did not damage the embedding medium or cellular detail.
D000330:Aedes / Q000648:ultrastructure; D000818:Animals; D011232:Chemical Precipitation; D008854:Microscopy, Electron; D010070:Oxalates; D013194:Staining and Labeling / Q000379:methods*; D014501:Uranium
D010070:Oxalates; D014501:Uranium