Immunofluorescence evidence for the absence of histone H1 in a mitotically dividing, genetically inactive nucleus.
Antibodies directed against whole histone and purified lysine-rich histone H1 extracted from isolated macronuclei of the ciliate Tetrahymena were obtained and conjugated to fluorescein isothiocyanate. The fluorescein-antibody conjugates were used to directly label Tetrahymena cells. Both macro- and micronuclei were visibly fluorescent in cells stained with anti-whole histone conjugate. However, the anti-H1 conjugate only labeled macronuclei. This in situ demonstration of the lack of positive immunofluorescent staining of micronuclei with anti-H1 conjugate provide further evidence for the absence of H1 in the genetically inactive, mitotically dividing Tetrahymena micronucleus.
D000166:Acridines; D000818:Animals; D002467:Cell Nucleus / Q000032:analysis; D005455:Fluorescent Antibody Technique; D006657:Histones / Q000032:analysis*; D008938:Mitosis; D013194:Staining and Labeling; D013769:Tetrahymena pyriformis / Q000032:analysis*
D000166:Acridines; D006657:Histones