Simplification of B-cell antisera of the Merrit system with platelets and lymphoblastoid cell lines.
Human sera with anti-HLA and anti-Merrit B-cell reactivities were absorbed with different volumes of a pool of packed outdated human platelets obtained from 237 donors. After absorption, each serum was tested against a panel of CLL cells and normal unfractionated peripheral blood lymphocytes that had given positive reactions with the same serum prior to absorption. The optimal platelet volume for complete absorption of anti-HLA and full preservation of anti-Merrit B-cell activities in these sera was found to be 1/10 to 1/5 of the volume of serum. Higher volumes of packed platelets, especially over 1/1, in most instances reduced anti-B-cell activity "nonspecifically." Among the platelet absorbed sera, those showing no HLA and strong anti-Merrit B-cell cytotoxicity were used for cross-absorption studies with cultured human lymphoid cell lines. Multi-specific anti-Merrit sera could be simplified by this method. Cultured lymphoblastoid cell lines represent a valuable resource, since large volumes of cells may be needed for absorption and simplification of human sera containing allogeneic B-lymphocyte antibodies.
D001402:B-Lymphocytes / Q000276:immunology*; D001792:Blood Platelets / Q000276:immunology*; D002460:Cell Line; D003601:Cytotoxicity Tests, Immunologic; D000939:Epitopes; D006680:HLA Antigens / Q000032:analysis; D006801:Humans; D007106:Immune Sera / Q000032:analysis*; D007164:Immunosorbents; D007945:Leukemia, Lymphoid / Q000276:immunology
D000939:Epitopes; D006680:HLA Antigens; D007106:Immune Sera; D007164:Immunosorbents