Studies of human IgM anti-IgG cryoglobulins: II. Isoelectric focusing characteristics of the reactive antigen and residual serum IgG pools.
The co-isoelectric focusing characteristics of the antigen-IgG and residual IgG pools in the serum of patients with mixed IgM:IgG cryoglobulinemia have been determined. The antigen IgG purified from five IgM cryoglobulins and the residual IgG purified from serum depleted of cryoglobulin were radiolabelled and co-isoelectric focused in polyacrylamide gels in order to determine if the IgM anti-globulin reacted in order to determine if the IgM anti-globulin reacted with IgGs having unique physicochemical properties. Electrofocusing profiles of the IgG from four sera showed only minor differences when the antigen-IgG was compared to residual serum IgG. In one instance, the IEF profile of the antigen-IgG pool was markedly restricted and of a different charge dispersion than the residual serum IgG. This correlated with the limited antigenic specificity previously shown for the autologous IgM antiglobulin.
D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D003450:Cryoglobulins / Q000276:immunology*; D000939:Epitopes; D006801:Humans; D007074:Immunoglobulin G / Q000276:immunology*; D007075:Immunoglobulin M / Q000276:immunology*; D007525:Isoelectric Focusing; D010265:Paraproteinemias / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D003450:Cryoglobulins; D000939:Epitopes; D007074:Immunoglobulin G; D007075:Immunoglobulin M