Simultaneous determination of human sperm morphology and viability: simple office technique.
Determinations of sperm morphology and viability are useful techniques for assessment of semen quality. In this report eosin-nigrosin is compared with hematoxylin-eosin for effectiveness in characterizing spermatozoa. The results indicate that eosin-nigrosin and hematoxylineosin give comparable results in differentiating spermatozoa. In addition, eosin-nigrosin allows the determination of the per cent of viable cells in each morphologic category. The eosin-nigrosin staining technique is a simple, rapid method which may give additional information in the assessment of male infertility.
D005069:Evaluation Studies as Topic; D005298:Fertility; D006652:Histological Techniques; D006801:Humans; D008297:Male; D012661:Semen / Q000166:cytology*; D013094:Spermatozoa / Q000166:cytology*; D013194:Staining and Labeling / Q000379:methods