Properties of a basement membrane-related glycoprotein synthesized in culture by a mouse embryonal carcinoma-derived cell line.
Two glycoproteins, GP-1 and GP-2, have been isolated from an extracellular membrane synthesized in cell culture by an embryonal carcinoma-derived cell line. The amino acid and carbohydrate compositions have been determined. Both proteins are rich in half-cystine residues and contain approximately 12-15% carbohydrate. Antibodies have been obtained against one of the glycoproteins, GP-2, in rabbits. The antibody reacts with basement membranes from adult mouse and human kidney glomeruli and tubules, and all basement membranes tested from mouse embryonic tissues. The molecular properties of GP-2 are superficially similar to LETS protein; however, immunological and other criteria show that they are distinct proteins. The presence of LETS protein and GP-2 in basement membranes suggests that there are subtle interactions which are important in adhesion of epithelial cells to basement membranes.
D000596:Amino Acids / Q000032:analysis; D000918:Antibody Specificity; D001485:Basement Membrane / Q000378:metabolism*; D002241:Carbohydrates / Q000032:analysis; D002460:Cell Line; D000939:Epitopes; D006023:Glycoproteins / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism; D009363:Neoplasm Proteins / Q000378:metabolism*; D013724:Teratoma / Q000378:metabolism*
D000596:Amino Acids; D002241:Carbohydrates; D000939:Epitopes; D006023:Glycoproteins; D008565:Membrane Proteins; D009363:Neoplasm Proteins