On the presence of reverse transcriptase in myelo- and lymphoproliferative disorders.
Buffy coats from 31 patients with a diagnosis of leukemia and 16 normal donors were tested for the presence of a viral-like reverse transcriptase. Eighty-five percent of fresh leukemic buffy coats were positive. Also tested were spleens from 16 patients with hematological disorders and 5 spleens from patients without history of hematological malignancy. The 5 normal spleens were negative. Also negative were 4 spleens from patients with Hairy cell leukemia. From the remaining 12 spleens 7 were positive. Reverse transcriptase measurements can be used to distinguish leukemic from normal buffy coats.
D003848:Deoxyguanine Nucleotides / Q000032:analysis; D006801:Humans; D007938:Leukemia / Q000201:enzymology*; D007943:Leukemia, Hairy Cell / Q000032:analysis / Q000201:enzymology; D009196:Myeloproliferative Disorders / Q000201:enzymology*; D012194:RNA-Directed DNA Polymerase / Q000032:analysis*; D013154:Spleen / Q000032:analysis
D003848:Deoxyguanine Nucleotides; D012194:RNA-Directed DNA Polymerase