Hydrodynamic orientation of sperm heads for flow cytometry.
Two specially shaped sample injection tubes orient flat-shaped mature sperm heads in a flow cytometer. Orientation allows control of a photometric difficulty experienced with some flow cytometers in the measurement of fluorescent and scattered light from sperm and other flat cells. Both relative DNA-stain content and a measure of cell flatness can now be determined simultaneously for each cell at the high rates possible with flow instruments.
D000818:Animals; D002417:Cattle; D002452:Cell Count / Q000295:instrumentation*; D006224:Cricetinae; D003584:Cytological Techniques; D004247:DNA / Q000032:analysis; D008297:Male; D051381:Rats; D012212:Rheology; D012756:Sheep; D013094:Spermatozoa / Q000166:cytology*; D013194:Staining and Labeling
D004247:DNA