[Cyto-immunologic location of alpha-MSH and ACTH in the lead-hematoxylin (HPb)-stainable pituitary cells, in the eel].
The lead-haematoxylin positive cells of the pars intermedia react with anti-alpha-MSH and anti-1-24ACTH or anti-17-39ATCH; those of the rostal pars distalis are only revealed with antisera anti-1-24ACTH and anti-17-39ACTH. Intensity of cytoimmunological staining, which is modified after various experimental treatments (reserpine, metyrapon, pimozid, cortisol...) and during black or white background adaptaiton, corresponds essentially to that of the PbH staining.
D000324:Adrenocorticotropic Hormone / Q000032:analysis*; D000811:Anguilla / Q000502:physiology*; D000818:Animals; D003594:Cytoplasmic Granules / Q000187:drug effects; D000939:Epitopes; D005455:Fluorescent Antibody Technique; D006416:Hematoxylin; D006854:Hydrocortisone / Q000494:pharmacology; D007124:Immunoenzyme Techniques; D008027:Light; D008297:Male; D009074:Melanocyte-Stimulating Hormones / Q000032:analysis*; D008797:Metyrapone / Q000494:pharmacology; D010868:Pimozide / Q000494:pharmacology; D010902:Pituitary Gland / Q000166:cytology* / Q000187:drug effects; D012110:Reserpine / Q000494:pharmacology; D012623:Seawater; D013194:Staining and Labeling
D000939:Epitopes; D010868:Pimozide; D012110:Reserpine; D000324:Adrenocorticotropic Hormone; D009074:Melanocyte-Stimulating Hormones; D006854:Hydrocortisone; D006416:Hematoxylin; D008797:Metyrapone
Localisation cyto-immunologique de alpha-MSH et d'ACTH dans les cellules hypophysaires colorables avec l'hématoxyline au plomb (HPb) chez l' anguille