Antigen recognition: the specificity of an isolated T lymphocyte population.
Guinea pig lymph node lymphocytes were separated into T and B cell fractions on immunoabsorbent columns. Separated cells were functionally distinct: T cells proliferated in response to ConA, PHA, soluble and alloantigen, whereas anti-Ig reagents only stimulated B cells. The in vitro proliferative response of guinea pig lymph node T lymphocytes was then shown to be highly discriminating when elicited by a series of structurally similar synthetic DNP-oligolysine antigens. Proliferation was always most extensive in response to the homologous, immunizing antigen, and less intense to cross-reacting DNP-oligolysines. Specificity of proliferation was maintained in the absence of both B lymphocytes and antibody secreting cells, suggesting that T cell recognition is not "acquired" from B cells or secreted antibody, but is a property inherent to the T cell.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis; D001402:B-Lymphocytes / Q000276:immunology; D002469:Cell Separation; D003208:Concanavalin A / Q000494:pharmacology; D004136:Dinitrobenzenes / Q000276:immunology; D000939:Epitopes; D006168:Guinea Pigs; D006462:Hemolytic Plaque Technique; D007519:Isoantigens; D037102:Lectins / Q000494:pharmacology; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008239:Lysine / Q000276:immunology; D010455:Peptides / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D004136:Dinitrobenzenes; D000939:Epitopes; D007519:Isoantigens; D037102:Lectins; D010455:Peptides; D003208:Concanavalin A; D008239:Lysine