Studies on H-2 specificities on mouse tumour cells by a new microradioassay.
Seven mouse tumour cell lines of different aetiology and origin were tested for the expression of surface alloantigens using twenty-four well defined H-2 alloantisera and anti-Thy 1.2. We used a new radioassay that involves antibody-complement treatment of the tumour target cells followed by postlabeling the surviving tumour cells with 14C-thymidine. With a relatively high frequency the anti-H-2 sera were reacting differently with the tumour cells than with respective syngeneic lymphoid cells. Thirty-six anomalous reactions out of 129 investigated were detected. Absorbtion experiments performed with H-2 antigen positive or negative lymphoid cells revealed a striking similarity between these extra-specificities and H-2 specificities of foreign haplotypes. The results are discussed in relation to the biological importance of the extra-specificities, both with regard to origin (derepression) and function (transplantation antigen properties).
D000818:Animals; D000912:Antibodies, Neoplasm; D000918:Antibody Specificity; D000951:Antigens, Neoplasm; D002462:Cell Membrane / Q000276:immunology; D002478:Cells, Cultured; D000939:Epitopes; D006649:Histocompatibility Antigens; D007109:Immunity; D051379:Mice; D009374:Neoplasms, Experimental / Q000276:immunology* / Q000378:metabolism; D011863:Radioimmunoassay; D013936:Thymidine / Q000378:metabolism
D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D000939:Epitopes; D006649:Histocompatibility Antigens; D013936:Thymidine