Studies on the action of myelin basic protein (MBP) in rat brain.
The specificity of I125-MBP uptake and subcellular distribution on a discontinuous sucrose density gradient, compared to those of histone H 4 and cytochrome c, showed a high affinity of MBP for mitochondria and heavy synaptosomes, and of histone H 4 for lighter synaptosomes. One heavier synaptosomal subpopulation was almost equally labelled by both proteins. Cytochrome c showed only a low uptake into particular material. Receptor interaction studies of MBP with H3-labelled 5-hydroxytryptamine and naloxone gave negative results.
D000818:Animals; D001667:Binding, Competitive; D001692:Biological Transport; D002540:Cerebral Cortex / Q000378:metabolism*; D003574:Cytochrome c Group / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D008297:Male; D008928:Mitochondria / Q000378:metabolism; D008970:Molecular Weight; D004676:Myelin Basic Protein / Q000378:metabolism*; D009270:Naloxone / Q000378:metabolism; D051381:Rats; D011985:Receptors, Serotonin / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology; D013574:Synaptosomes / Q000378:metabolism
D003574:Cytochrome c Group; D006657:Histones; D004676:Myelin Basic Protein; D011985:Receptors, Serotonin; D009270:Naloxone; D012965:Sodium Chloride