Leucocyte adherence inhibition test in arthritis.
Peripheral leucocytes from healthy blood-donors were preincubated in polyarthritic serum or normal serum and their adherence to glass tubes in the presence of synovial extract of control extract was measured. Serum from patients with inflammatory polyarthritis significantly reduced the proportion of leucocytes which adhered in the presence of synovial extract, but not control extract. Serum from patients with polyarthritis with visceral manifestations, patients with degenerative or metabolic polyarthritis or rheumatic fever, and controls did not have this effect. The leucocyte sensitising activity was eluted in the IgG fraction of serum, and could be absorbed by both synovial extract and IgG. It is thus probably IgG rheumatoid factor. In vivo, armed leucocytes in polyarthritic patients may bind to synovial tissue initiating or perpetuating the articular inflammatory process.
D000328:Adult; D000368:Aged; D001168:Arthritis / Q000175:diagnosis / Q000276:immunology*; D001327:Autoimmune Diseases / Q000175:diagnosis*; D001666:Binding Sites, Antibody; D005260:Female; D005898:Glass; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D007074:Immunoglobulin G; D007158:Immunologic Techniques; D007957:Leukocyte Adherence Inhibition Test; D008297:Male; D008875:Middle Aged; D012217:Rheumatoid Factor; D013583:Synovial Membrane / Q000276:immunology; D014020:Tissue Extracts
D007106:Immune Sera; D007074:Immunoglobulin G; D014020:Tissue Extracts; D012217:Rheumatoid Factor