Localization chromatid breaks in Fanconi's anemia, using three consecutive stains.
The location of 339 break points was analyzed in three patients with Fanconi's anemia, using three consecutive stains: ordinary Giemsa, Q-banding, and R-banding. Almost all the breaks seem to take place in the Q bands, using R-banding, and in the R bands, using Q-banding. A very important artifact, varying according to the method used, is thus demonstrated. In fact, the breaks take place in the interbands, between R and Q bands. The breaks were also localized in relation to sister chromatid exchanges (SCEs), seen after BUDR treatment. There is a clear excess of breaks at place of SCE (29%). This may indicate a possible correlation between breaks and SCEs.
D000741:Anemia, Aplastic / Q000235:genetics*; D002842:Chromatids; D002869:Chromosome Aberrations; D005199:Fanconi Anemia / Q000235:genetics*; D006801:Humans; D007621:Karyotyping; D008297:Male; D013194:Staining and Labeling