Tolerance induction in maturing B cells.
A two-stage tissue culture system was used to test the concept of clonal abortion as a mechanism for tolerance induction in B cells. In the first stage, neonatal spleen cells or bone marrow cells were cultured for 72 h under conditions in which B-cell neogenesis occurred. Haptens coupled to various carriers, were introduced during this stage. Following this culture phase, the cells were washed and their competence to respond to hapten-POL was measured in microcultures where feedback effects were minimized. The results indicated that immature B cells were specially susceptible to tolerance but that the conditions under which hapten was presented were also important in determining the outcome of the cell-antigen encounter.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D001853:Bone Marrow / Q000254:growth & development; D001854:Bone Marrow Cells; D002352:Carrier Proteins; D002478:Cells, Cultured; D004306:Dose-Response Relationship, Immunologic; D000939:Epitopes; D006241:Haptens; D006462:Hemolytic Plaque Technique; D007108:Immune Tolerance; D051379:Mice
D002352:Carrier Proteins; D000939:Epitopes; D006241:Haptens