A mucoprotein with colon-specific determinants.
The tissue specificity of colonic mucoprotein antigen (CMA) was examined to determine whether the antigen might have a role as a tissue marker. The immunofluorescent technique, using a rabbit anti-CMA antiserum, was employed to examine a wide range of tissues. Gastrointestinal mucosae as well as non-gastrointestinal mucin producing tissues were positive; however, non-gastrointestinal reactivity could be eliminated by the appropriate absorptions. Mucoproteins were purified separately from each gastrointestinal anatomic region and used to absorb the antiserum. This analysis demonstrated the existence of a family of mucoproteins with a common gastrointestinal specific determinant(s). Mucoproteins from neighboring regions, as seen by this antiserum, showed a greater structural similarity than did mucoproteins from distant regions. Absorption of the antiserum with ileal or cecal mucoprotein allowed the detection of a colon specific determinant present on CMA.
D003106:Colon / Q000276:immunology*; D000939:Epitopes; D005455:Fluorescent Antibody Technique; D006801:Humans; D007106:Immune Sera; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007413:Intestinal Mucosa / Q000276:immunology; D009088:Mucoproteins / Q000276:immunology*
D000939:Epitopes; D007106:Immune Sera; D009088:Mucoproteins