title: Surface expression of murine leukemia virus structural polypeptides on host cells and the virion.
abstract: The expression of murine leukemia virus structural polypeptides on the surface of cells producing exogenous Friend leukemia virus, endogenous ecotropic AKR and xenotropic BALB/c virus was investigated. Antisera to Friend virus gp71, p30, p15E, p12 and p10 were employed in a complement-dependent chromium release assay and to immunoprecipitate lactoperoxidase iodinated surface polypeptides prior to analysis in polyacrylamide gel electrophoresis. With the latter technique gag-gene encoded proteins and their precursors were not discovered on the viral and cellular surface membranes. Only env-gene encoded polypeptides gp85, gp71, and p15E were detectable. p15E is embedded into the lipid membrane. gp85 is formed by disulfide linkage of p15E to surface-exposed gp71. The ratio of gp71 to gp85 is variable and apparently determined by the host cell. Antibodies of strong cytotoxicity are those against type- and group-specific epitopes of gp71 as well as type-specific epitopes of p12.
mesh_terms: D000914:Antibodies, Viral; D000954:Antigens, Surface; D000956:Antigens, Viral; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D003602:Cytotoxicity, Immunologic; D000939:Epitopes; D005622:Friend murine leukemia virus / Q000276:immunology; D009052:Leukemia Virus, Murine / Q000276:immunology* / Q000378:metabolism; D010452:Peptide Biosynthesis; D010455:Peptides / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D000954:Antigens, Surface; D000956:Antigens, Viral; D000939:Epitopes; D010455:Peptides