In vitro culture of human prostatic tissue.
A procedure is described which yields a significant percentage of long-term mixed cell cultures of human prostatic tissue. Attempts were made to suppress the proliferation of stromal fibroblasts and to characterize the cultured cells as those of prostatic origin. The problems associated with establishing epithelial cell lines are discussed.
D000135:Acid Phosphatase / Q000032:analysis; D002478:Cells, Cultured; D004847:Epithelial Cells; D004848:Epithelium / Q000201:enzymology; D005347:Fibroblasts; D006801:Humans; D008297:Male; D008722:Methods; D003012:Microbial Collagenase / Q000494:pharmacology; D011470:Prostatic Hyperplasia / Q000201:enzymology / Q000473:pathology*; D011471:Prostatic Neoplasms / Q000201:enzymology / Q000473:pathology*
D000135:Acid Phosphatase; D003012:Microbial Collagenase