[Modification of sialic acid determination in erythrocytes].
Various chromophores impair the evaluation of N-acetyl-neuraminic acid (NANA) of red blood cells by means of the thiobarbituric acid method according to WARREN. Therefore, a time consuming isolation of erythrocyte membranes is essential in order to avoid these shortcomings. The procedure was simplified by fixation with glutaraldehyde of washed red blood cells.
D004910:Erythrocyte Membrane; D004912:Erythrocytes / Q000032:analysis*; D006651:Histocytochemistry / Q000379:methods*; D006801:Humans; D006868:Hydrolysis; D012794:Sialic Acids / Q000097:blood*; D013858:Thiobarbiturates
D012794:Sialic Acids; D013858:Thiobarbiturates
Eine Modifikation der Sialinsäure-Bestimmung am Erythrozyten.