Preparation and antigenic properties of 5alpha-dihydrotestosterone-11-(O-carboxymethyl) oxime-BSA conjugate.
The C-11 (O-carboxymethyl) oxime derivative of 5-alphadihydrotestosterone (5alphaDHT) has been prepared. Due to steric hindrance at C-11, a novel two step procedure was used to introduce the (O-carboxymethyl) oxime at this position. Condensation of this oxime to bovine serum albumin afforded a conjugate which produced anti-5alphaDHT sera inoculated rabbits. Apart from a 30% cross reaction with testosterone, the antisera was reasonably specific for 5alphaDHT.
D000818:Animals; D003429:Cross Reactions; D013196:Dihydrotestosterone / Q000031:analogs & derivatives* / Q000276:immunology; D000939:Epitopes; D007106:Immune Sera; D008297:Male; D011817:Rabbits; D012710:Serum Albumin, Bovine
D000939:Epitopes; D007106:Immune Sera; D013196:Dihydrotestosterone; D012710:Serum Albumin, Bovine