title: Recognition sequence of restriction endonuclease KpnI from Klebsiella pneumoniae.
abstract: We have determined the recognition sequence of the restriction endonuclease KpnI, previously isolated from Klebsiella pneumoniae. The enzyme cleaves the twofold rotationally symmetric sequence (see book for formula) at the positions indicated by the arrows, producing 3' protruding cohesive ends, four nucleotides in length. The specific cleavage site was unambiguously deduced using both 3' and 5' end analyses of KpnI generated restriction fragments of simian-virus 40 (SV40) DNA (1 site), adenovirus-2 (Ad-2) DNA (8 sites), and a plasmid (pCRI) DNA (2 sites).
mesh_terms: D009189:Avian Myeloblastosis Virus / Q000201:enzymology; D001483:Base Sequence; D004247:DNA; D004262:DNA Restriction Enzymes; D003851:Deoxyribonucleases; D003854:Deoxyribonucleotides / Q000032:analysis; D007711:Klebsiella pneumoniae / Q000201:enzymology*; D010727:Phosphoric Diester Hydrolases; D012194:RNA-Directed DNA Polymerase
chemical_list: D003854:Deoxyribonucleotides; D004247:DNA; D012194:RNA-Directed DNA Polymerase; D003851:Deoxyribonucleases; D004262:DNA Restriction Enzymes; D010727:Phosphoric Diester Hydrolases