Induction of guinea pig antibody responses in vitro.
Guinea pig spleen cells cultured together with peritoneal exudate lymphocytes (PEL) were found to generate large numbers of antibody-forming cells (AFC) in vitro in response to hapten-protein antigens. Neither cell type cultured alone yielded appreciable responses. Strain 13 or F1 (Strain 2 X Strain 13) lymphocytes, but not those from strain 2 animals, are able to respond to the genetically controlled antigen, DNP-guinea pig albumin (DNP-GPA). Antisera directed against responder (strain 13) parent Ia antigens selectively blocked the generation of AFC by F1 (strain 2 X strain 13) spleen-PEL mixtures in response to DNP-GPA. Both allogeneic (strain 2) and syngeneic macrophages functioned equally well in presentation of DNP-GPA to strain 13 lymphocytes.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D000917:Antibody Formation; D000921:Antibody-Producing Cells / Q000276:immunology; D000941:Antigens; D001202:Ascitic Fluid / Q000276:immunology; D004136:Dinitrobenzenes / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D000939:Epitopes; D006168:Guinea Pigs; D006649:Histocompatibility Antigens; D008214:Lymphocytes / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D012709:Serum Albumin / Q000276:immunology
D000906:Antibodies; D000941:Antigens; D004136:Dinitrobenzenes; D000939:Epitopes; D006649:Histocompatibility Antigens; D012709:Serum Albumin