[Immunogenicity of isocomponents of the alpha-toxoid of Cl. oedematiens for mice of opposite reacting genotypes].
There was revealed molecular heterogeneity of the highly purified Cl. oedematiens alpha-toxoid. By the method of isoelectrical focussing alpha-toxoid was divided into isocomponents with the isoelectrical points of 5.33 +/- 0.02 and 4.91 +/- 0.08 with an equal specific activity and serological specificity. In studying the immunogenicity of alpha-toxoid and its isocomponents in experiments on mice of oppositely reacting genotypes it was shown that for mice of the low reacting strain (DBA/2) the the alpha-toxoid isocomponents possessed a much greater immunogenicity than the highly purified alpha-toxoid; under the same conditions the immunogenicity of the alpha-toxoid isocomponents for mice of the highly reacting strain (CBA) failed to differ from the immunogenicity of the highly purified alpha-toxoid.
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D003013:Clostridium / Q000276:immunology*; D003015:Clostridium Infections / Q000276:immunology / Q000517:prevention & control*; D000939:Epitopes; D007525:Isoelectric Focusing; D051379:Mice; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D013045:Species Specificity; D014121:Toxoids / Q000008:administration & dosage
D000907:Antibodies, Bacterial; D000939:Epitopes; D014121:Toxoids
Immunogennost' izokomponentov alpha-anatoksina Cl. oedematiens dlia mysheĭ oppozitno reagiruiushchikh genotipov.