M467: a murine IgA myeloma protein that binds a bacterial protein. I. Recognition of common antigenic determinants on Salmonella flagellins.
We have studied the binding of M467, an IgA murine myeloma protein, to flagellin from seven species of Salmonella. It was found that M467 was reacting with antigenic determinants that were common to all the flagellins studied. These determinants were not related to serotypic antigens. Electronmicrographs of unreduced M467 showed a variety of polymeric species bound to flagella in a manner that could produce immobilization as well as agglutination and precipitation through cross-linking of antigenic determinants. Immunodiffusion in agar gel revealed that M467 was recognizing more than one group of peptide determinants on the flagellins studied. Passive hemagglutination inhibition and a solid phase radioimmunoassay provided evidence that there were differences in binding avidities between M467 and the various Salmonella flagellins studied. It was concluded that M467 is binding more than one specific group of antigenic peptide determinants on flagellin molecules. Flagellin from four of the seven species of Salmonella studied were deficient in one or more of these determinants.
D000818:Animals; D001426:Bacterial Proteins / Q000276:immunology* / Q000378:metabolism*; D000939:Epitopes; D005408:Flagellin / Q000276:immunology*; D006385:Hemagglutination Inhibition Tests; D005779:Immunodiffusion; D007070:Immunoglobulin A / Q000378:metabolism*; D051379:Mice; D009194:Myeloma Proteins / Q000378:metabolism*; D011485:Protein Binding; D012475:Salmonella / Q000276:immunology* / Q000648:ultrastructure
D001426:Bacterial Proteins; D000939:Epitopes; D007070:Immunoglobulin A; D009194:Myeloma Proteins; C020957:myeloma protein M 467; D005408:Flagellin