Radioimmunoassay of alpha-fetoprotein, with special reference to iodination and purification techniques.
We report a relatively simple and convenient method for iodinating human alpha-fetoprotein and for purifying the 125I-labeled material. The label is incorporated into human alpha-fetoprotein enzymatically by use of lactoperoxidase. Before each assay the labeled material is purified over two successive short columns: Sepharcryl S-200 Superfine and cellulose. This procedure removes both free iodine and "damaged" fetoprotein. With the purified material we developed a sensitive and reliable radioimmunoassay for alpha-fetoprotein in serum and amniotic fluid.
D000653:Amniotic Fluid / Q000032:analysis*; D002850:Chromatography, Gel; D005260:Female; D006801:Humans; D007457:Iodine Radioisotopes; D007784:Lactoperoxidase; D008431:Maternal-Fetal Exchange; D011247:Pregnancy; D011863:Radioimmunoassay / Q000379:methods; D000509:alpha-Fetoproteins / Q000032:analysis* / Q000097:blood / Q000302:isolation & purification
D007457:Iodine Radioisotopes; D000509:alpha-Fetoproteins; D007784:Lactoperoxidase