Antigen-induced release of histamine from passively sensitized guinea pig lung slices. I. Optimum conditions for in vitro passive sensitization and challenge with antigen.
The effect of several parameters (pH, Ca++ concentration, time, temperature, lung slice size) on in vitro antigen-induced histamine release from passively sensitized guinea pig lung slices was investigated. With respect to pH, Ca++ concentration and time, the optimal conditions for the passive sensitization step (pH 7.8, Ca++ 1.5 mM, 2 h) were distinctly different from those for the antigen challenge step (pH 8.2, Ca++ 10mM, 15 min). Maximum antigen-induced release was obtained with 3 X 0.25 X 0.25 mm lung slices at 37 degrees C using air as gas phase.
D000388:Air; D000818:Animals; D000941:Antigens / Q000008:administration & dosage*; D002118:Calcium / Q000378:metabolism; D002245:Carbon Dioxide; D005260:Female; D006168:Guinea Pigs; D006636:Histamine Release; D006863:Hydrogen-Ion Concentration; D007112:Immunity, Maternally-Acquired; D007114:Immunization; D008168:Lung / Q000276:immunology*; D010100:Oxygen; D013696:Temperature; D013997:Time Factors
D000941:Antigens; D002245:Carbon Dioxide; D010100:Oxygen; D002118:Calcium