title: [Nature of mutations disrupting the formation of 6-phosphogluconate dehydrogenase in Drosophila melanogaster, and their suppression].
abstract: Molecular nature of lethal and semilethal mutations in the Pgd locus of D. melanogaster coding for 6-phosphogluconate dehydrogenase (6tpgd) was studied. All these mutations affect the structural gene of the Pgd locus: 3 semilethal mutations resulted in altered 6PGD molecules with the decreased catalytic activity; the remaining 8 lethals were "zero" alleles possessing mutant polypeptides inactive but capable to react with antisera against highly purified 6PGD. "Zero" or low activity alleles for glucose-6-phosphate dehydrogenase induced by ethyl methansulfonate were shown to be supressors for the lethal mutations in the Pgd locus. A monocistronic type of organization of the Pgd locus is suggested taking into account the biochemical mechanism of supression of the Pgd-lethals and their location in the structural gene coding for 6GPD.
mesh_terms: D000483:Alleles; D000818:Animals; D002384:Catalysis; D004331:Drosophila melanogaster / Q000235:genetics; D000939:Epitopes; D005020:Ethyl Methanesulfonate / Q000506:poisoning; D005260:Female; D005796:Genes; D005804:Genes, Lethal; D005779:Immunodiffusion; D007527:Isoenzymes / Q000235:genetics* / Q000276:immunology; D008297:Male; D009154:Mutation; D010734:Phosphogluconate Dehydrogenase / Q000235:genetics* / Q000276:immunology; D013489:Suppression, Genetic
chemical_list: D000939:Epitopes; D007527:Isoenzymes; D005020:Ethyl Methanesulfonate; D010734:Phosphogluconate Dehydrogenase
vernacular_title: Priroda mutatsiĭ, narushaiushchikh obrazovanie 6-fosfogliukonatdegidrogenazy u Drosophila melanogaster, i ikh supressiia.