Preparation of the major plasma fractions by solid-phase polyelectrolytes.
The solid-phase ethylene/maleic anhydride PE's E-5 and E-100 were used to developed a flexible integrated system of batch methods for the fractionation of albumin and gamma globulin as well as the clinically important coagulation factors. Each method for the isolation and purification of the fractions or a modification of it could also be used independently or could be added onto another method or methods. Each fraction was prepared in three steps: adsorption on solid-phase PE's elution from the PE's, and concentration by ultrafiltration or by use of PEG or both. Since the methods required no organic solvent but relied primarily on electrostatic bonding, they were essentially nondenaturing. Solid-liquid phase separation was easily accomplished by centrifugation or filtration. The yield and purity of each fraction was as follows: (1) coagulation factors II, VII, IX, and X concentrates 63% and 200-fold purified; (2) AHF 43% and 52-fold; (3) vWF 43% and 71-fold; (4) PPF, as albumin, 93% yield and 92% pure; (5) albumin 92% yield and 98.5% pure; and (6) gamma globulin 92% yield and 97.5 pure. During the preparation of gamma globulin the E-100 also tended to adsorb and remove the HBSAg and infectivity, when present.
D001779:Blood Coagulation Factors / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005030:Ethylenes; D005590:Fractional Precipitation; D005663:Furans; D006801:Humans; D007122:Immunoelectrophoresis; D008299:Maleic Anhydrides; D012709:Serum Albumin / Q000302:isolation & purification*; D005719:gamma-Globulins / Q000302:isolation & purification*
D001779:Blood Coagulation Factors; D005030:Ethylenes; D005663:Furans; D008299:Maleic Anhydrides; D012709:Serum Albumin; D005719:gamma-Globulins