Fixation of mammalian tissues in different fixatives and its influence on the staining with methyl green-pyronin.
This investigation is a study on the role of fixation of different mammalian tissues in different fixatives as well as in 10% neutral formalin containing different metal cations and its effect on the staining with the dye-mixture, methyl green-pyronin. The results indicate variation in the colour of the nuclei in tissues fixed in the different fixatives. The possible role of the fixatives on nuclear colouration has been discussed.
D000818:Animals; D002467:Cell Nucleus / Q000032:analysis; D005404:Fixatives; D008739:Methyl Green; D009928:Organ Specificity; D011754:Pyronine; D051381:Rats; D012394:Rosaniline Dyes; D013194:Staining and Labeling / Q000379:methods*; D014966:Xanthenes
D005404:Fixatives; D012394:Rosaniline Dyes; D014966:Xanthenes; D008739:Methyl Green; D011754:Pyronine