[Histofluorescence of catecholamines and visualization of retrograde transported peroxidase in the same tissue section and in the same neuron].
The demonstration of fluorescent catecholamines and horseradish peroxidase (HRP) in the same neuron has been achieved in the Rat in two ways: by submitting vibratome sections to a modified glyoxylic acid fluorescence method followed by the usual procedure to reveal HRP; or by combining the last procedure with the cryostat technique of Chiba et coll. After HRP injection into the striatum or the nucleus accumbens of the Rat, non-fluorescent HRP labelled neurons were observed in the substantia nigra and the ventral tegmental area respectively, in addition to the HRP labelled fluorescent dopaminergic neurons.
D000818:Animals; D001370:Axonal Transport; D002395:Catecholamines / Q000378:metabolism*; D006735:Horseradish Peroxidase / Q000378:metabolism*; D008297:Male; D008856:Microscopy, Fluorescence; D009474:Neurons / Q000378:metabolism*; D010544:Peroxidases / Q000378:metabolism*; D051381:Rats; D013378:Substantia Nigra / Q000378:metabolism
D002395:Catecholamines; D006735:Horseradish Peroxidase; D010544:Peroxidases
Histofluorescence des catécholamines et révélation de la peroxydase transportée par voie rétrograde sur une même coupe de tissu et dans le même neurone.