beta2-Microglobulin from normal and leukaemic guinea-pig lymphocytes.
beta2-Microglobulin has been isolated in useful quantities from the urine of strain-2 guinea-pigs after either treatment with sodium chromate or induction of the L2C leukaemia. Antibodies raised against the beta2-microglobulin were used to set up a radioimmunoassay which measured its export into culture fluid by normal and leukaemic lymphocytes. Material containing beta2-microglobulin was also obtained by digestion of the lymphocytic surfaces with papain; fractionation demonstrated both free and combined forms, with no qualitative difference between those from normal and those from leukaemic cells.
D000818:Animals; D000941:Antigens; D001609:Beta-Globulins / Q000302:isolation & purification*; D002478:Cells, Cultured; D002850:Chromatography, Gel; D006168:Guinea Pigs; D007942:Leukemia, Experimental / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology*; D010206:Papain / Q000494:pharmacology; D001613:beta 2-Microglobulin / Q000096:biosynthesis / Q000276:immunology / Q000302:isolation & purification*
D000941:Antigens; D001609:Beta-Globulins; D001613:beta 2-Microglobulin; D010206:Papain