A combined assay for the rapid preliminary detection of structural retroviruses.
This report describes the use of equilibrium gradients, RNA dependent DNA polymerase assays and electron microscopy (EM) in a combined assay for the rapid preliminary detection of intact retroviruses in crude preparations. Positive combined assays of platelets from preleukemic patients corresponded with karyotypic abnormalities found in these patients. Reconstruction experiments with Rauscher Leukemia Virus added to buffer or disrupted mouse spleen demonstrated the ease of detecting 10(9) or greater particles/g crude tissue, and the effects of buffer or added protein.
D000818:Animals; D001792:Blood Platelets / Q000382:microbiology; D006801:Humans; D007181:Inclusion Bodies, Viral; D007942:Leukemia, Experimental / Q000382:microbiology; D051379:Mice; D008854:Microscopy, Electron; D011289:Preleukemia / Q000201:enzymology / Q000382:microbiology* / Q000648:ultrastructure; D012194:RNA-Directed DNA Polymerase / Q000032:analysis; D011925:Rauscher Virus / Q000302:isolation & purification; D012190:Retroviridae / Q000201:enzymology / Q000302:isolation & purification*; D013154:Spleen / Q000382:microbiology
D012194:RNA-Directed DNA Polymerase