Influence of Q- and G-banding on the Feulgen-stainability of human metaphase chromosomes.
In this paper, model experiments on chicken red blood cell nuclei are described concerning the influence of methanol-acetic acid fixation and irradiation at different wavelengths, with and without prior Atebrin staining on subsequent Feulgen-stainability. In addition, data are reported on the influence on Feulgen-stainability of Giemsa-banding procedures, illumination of unstained chromosomes at 220 and 515 nm and exposure of unstained and Atebrin-stained chromosomes to illumination at 440 nm. The ASG and especially the trypsin-Giemsa technique appeared to reduce markedly Feulgen-stainability. The same holds true for Atebrin fluorescence of chromosomes. The data are discussed in relation to their implications for the assumed cause of the Q- and G-banding phenomena. Techniques are described that allow reliable Feulgen DNA measurements of individual chromosomes after application of either G- or Q-banding.
D000818:Animals; D001399:Azure Stains; D002645:Chickens; D002875:Chromosomes / Q000032:analysis* / Q000528:radiation effects; D004247:DNA / Q000032:analysis*; D005404:Fixatives; D006801:Humans; D008027:Light; D008938:Mitosis; D011797:Quinacrine Mustard; D013194:Staining and Labeling; D014466:Ultraviolet Rays
D001399:Azure Stains; D005404:Fixatives; D011797:Quinacrine Mustard; D004247:DNA