Human complement activation by lipopolysaccharides from bacteroides oralis, fusobacterium nucleatum, and veillonella parvula.
The properties of different lipopolysaccharide (LPS) preparations to induce C3 conversion in human serum was studied by means of crossed immunoelectrophoresis. C3 conversion by the alternative pathway was evaluated after calcium depletion, and lipid A-dependent activation was measured by means of inhibition with polymyxin B sulfate. LPS from Bacteroides oralis converted Co mainly via the alternative pathway, whereas LPS from Fusobacterium nucleatum and Veillonella parvula const pronounced lipid A-dependent conversion. The results are discussed in relation to the chemical composition of the LPS preparations.
D000328:Adult; D000936:Antigen-Antibody Complex; D001439:Bacteroides / Q000276:immunology*; D003167:Complement Activation; D003176:Complement C3 / Q000276:immunology*; D004492:Edetic Acid / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D005673:Fusobacterium / Q000276:immunology*; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D008070:Lipopolysaccharides / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D011112:Polymyxin B / Q000494:pharmacology; D013045:Species Specificity; D014678:Veillonella / Q000276:immunology*; D015054:Zymosan / Q000276:immunology
D000936:Antigen-Antibody Complex; D003176:Complement C3; D008070:Lipopolysaccharides; D004533:Egtazic Acid; D015054:Zymosan; D004492:Edetic Acid; D011112:Polymyxin B