[Antibody response to somatic and flagellar antigens of Pseudomonas aeruginosa (author's transl)].
Using the indirect fluorescent antibody technique, the production of antiflagellar and antisomatic antibodies in rabbits immunized with different P. aeruginosa strains is measured. After 6 injections of formalinized cultures in graded doses over 3 weeks, the antiflagellar antibodies reach the peak in the 4th to 5th week, followed by a rapid fall. The antisomatic antibodies reacting with native and boiled cells show a plateau-shaped maximum from the 3rd to 5th/6th week and fell slightly up to the 11th week. A significant dissociation of antiflagellar and antisomatic antibodies, however, is not observed. The antisomatic antibodies are less absorbed by protein A (Staph. aureus) than are the antiflagellar antibodies.
D000042:Absorption; D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis* / Q000276:immunology; D000918:Antibody Specificity; D000942:Antigens, Bacterial; D005407:Flagella / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D007114:Immunization; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D011817:Rabbits; D013205:Staphylococcal Protein A / Q000276:immunology
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D013205:Staphylococcal Protein A
Antikörperbildung gegen somatische und flagelläre Antigene von Pseudomonas aeruginosa.