[Development and evaluation of a method of high pressure liquid chromatography for the determination of the level of degradation of thyroliberin (TRH) in blood].
The degradation of the TRH by plasma was studied using a sensitive method for the separation of TRH and products formed by high-pressure liquid chromatographic analysis (HPLC). The detected products are TRH-3H or synthetic TRH. The HPLC analysis is performed on a microparticulate (10 mu) silica gel chromatographic column with CH3CN/0,01 M NH4OAc, at pH 6 (30/70) (V/V). This technique provides a good separation of TRH and the other products of degradation. In our experimental conditions, the human plasma degrades 62.5 +/- 5% of of TRH in 60 mn; very similar results are obtained with thin layer chromatography.
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D007700:Kinetics; D013973:Thyrotropin-Releasing Hormone / Q000097:blood* / Q000378:metabolism
D013973:Thyrotropin-Releasing Hormone
Développement et évaluation d'une méthode par chromatographie liquid à haute pression pour la détermination du taux de dégradation de la thyréolibérine (TRH) par le plasma.