[The complexing ability of gangliosides for Ca2, I. Influence of mono- and divalent cations and of acetylcholin (author's transl)].
The binding of Ca2 to single ganglioside species (GGtet1NeuAc, GGtet2aNeuAc, GGtet 3aNeuAc), to their free, reducing sialyl-oligosaccharides and to ganglioside mixtures from chicken brain was investigated by means of ion-sensitive electrodes (potentiometry). Unlike the sialyl-oligosaccharides and free N-acetylneuraminic acid, gangliosides were found to possess two different modes of binding for Ca2, depending on the total concentration of Ca2. This was mainly indicated by a release of up to 75% of previously bound Ca2 after raising the total Ca2-concentration above a critical level of about 5--9 X 10(-5)M. Addition of acetylcholin (9mM), Li, K, and Na, respectively, caused a release of Ca2 from ganglioside-Ca2-complexes (0.1mM Ca2, 0.1mM ganglioside-NeuAc) in the sequence: acetylcholin : Li : K : Na = 4 : 3 : 1 : 1 composed on molar basis (9mM).
D000109:Acetylcholine; D002118:Calcium; D002412:Cations; D055598:Chemical Phenomena; D002621:Chemistry; D004533:Egtazic Acid; D005732:Gangliosides; D007700:Kinetics; D013329:Structure-Activity Relationship
D002412:Cations; D005732:Gangliosides; D004533:Egtazic Acid; D000109:Acetylcholine; D002118:Calcium
Das Komplexationsvermögen von Gangliosiden für Ca2, I. Einfluss mono- und divalenter Kationen sowie von Acetylcholin.