[Permanent cell culture of a pancreatic carcinoma induced by immunological effect. Comparison of the evolution of secretory specificity and oncogenic power in two homologous strains: in vitro (147-8) and in vivo (7-4)].
A permanent epithelial cell strain, named 147-8, was established in vitro from a pancreatic carcinoma immunologically induced in a mouse. The cells remain isolated and grow actively in suspension: after more than 3 years of life, the doubling time is 14 hrs. Some cells synthetized insulin during the first two months. Later on, the cells contain low but significant levels of amylase and lipase, even during the second year, thus showing some pancreatic specificity. The oncogenic property of this strain is high during the first two years, and later decreases while their multiplication rate remains high. The evolution of 147-8 strain is compared to that of its in vivo homologous strain 7-4.
D000681:Amylases / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation; D046508:Culture Techniques; D007106:Immune Sera; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D008049:Lipase / Q000378:metabolism*; D051379:Mice; D009374:Neoplasms, Experimental / Q000276:immunology / Q000378:metabolism; D010190:Pancreatic Neoplasms / Q000276:immunology / Q000378:metabolism*
D007106:Immune Sera; D007328:Insulin; D008049:Lipase; D000681:Amylases
Culture cellulaire permanente de carcinome du pancréas induit par effet immunologique. Comparaison de l'évolution de la spécificité sécrétoire et du pouvoir oncogène dans deux souches homologues: in vitro (147-8) et in vivo (7-4).