Inhibition of bacterial transport by uncouplers of oxidative phosphorylation. Effects of pentachlorophenol and analogues in Bacillus subtilis.
Analogues of the potent uncoupler of oxidative phosphorylation pentachlorophenol were tested as inhibitors of proline and glycine transport by Bacillus subtilis. These analogues included less highly substituted chlorophenols and pentachlorothiophenol. Like pentachlorophenol, they are non-competitive inhibitors of proline transport and uncompetitive inhibitors of glycine transport. However, the less highly substituted chlorophenols are weaker acids than pentachlorophenol and also weaker inhibitors. Analysis indicated that the anionic form of the uncouplers is the inhibiting species. Pentachlorothiophenol, a water-insoluble anion, is also a potent inhibitor. These results support previous studies that concluded that uncouplers of oxidative phosphorylation inhibit amino acid transport by binding at specific sites on proteins, the free energy of interaction stabilizing 'unproductive' conformations. Such specific interactions of uncoupler with protein are probably commonplace.
D001412:Bacillus subtilis / Q000187:drug effects* / Q000378:metabolism; D001692:Biological Transport / Q000187:drug effects; D002462:Cell Membrane / Q000378:metabolism; D002733:Chlorophenols / Q000494:pharmacology*; D005998:Glycine / Q000378:metabolism; D007700:Kinetics; D010416:Pentachlorophenol / Q000031:analogs & derivatives / Q000494:pharmacology*; D011392:Proline / Q000378:metabolism; D014475:Uncoupling Agents / Q000494:pharmacology*
D002733:Chlorophenols; D014475:Uncoupling Agents; D011392:Proline; D010416:Pentachlorophenol; D005998:Glycine