The main neutral protease of rat skin is a mast cell enzyme. Immunohistochemical localization of the enzyme in rat skin with the peroxidase-antiperoxidase (PAP) complex method.
The highly sensitive PAP immunoperoxidase method was used to localize the main neutral protease of rat skin. The use of the neutral detergent, Triton X-100, in the reagent and washing solutions was observed to effectively decrease the nonspecific staining. The specific staining was localized to the mast cell granules.
D000818:Animals; D005260:Female; D006735:Horseradish Peroxidase; D007124:Immunoenzyme Techniques; D008297:Male; D008407:Mast Cells / Q000201:enzymology* / Q000648:ultrastructure; D010447:Peptide Hydrolases / Q000032:analysis*; D051381:Rats; D012867:Skin / Q000201:enzymology* / Q000648:ultrastructure
D006735:Horseradish Peroxidase; D010447:Peptide Hydrolases