title: An oviducal enzyme isolated by affinity chromatography which acts upon the vitelline envelope of Bufo arenarum coelomic oocytes.
abstract: A trypsin-like oviducal proteinase acting upon the vitelline envelope of Bufo arenarum coelomic oocytes has been purified to apparent homogeneity by gel filtration on Sephadex G-200 and by affinity chromatography on a column of Sepharose 4-B containing covalently bound concanavlin A (Con A). The biologically active molecule migrated as a single band of protein upon SDS polyacrylamide gel electrophoresis.
mesh_terms: D000818:Animals; D002022:Bufo arenarum; D002846:Chromatography, Affinity / Q000379:methods; D003208:Concanavalin A; D010450:Endopeptidases / Q000302:isolation & purification* / Q000378:metabolism; D005260:Female; D009865:Oocytes / Q000378:metabolism*; D010057:Oviducts / Q000201:enzymology*; D010063:Ovum / Q000378:metabolism*; D014357:Trypsin; D014817:Vitelline Membrane / Q000378:metabolism
chemical_list: D003208:Concanavalin A; D010450:Endopeptidases; D014357:Trypsin