Anti-actin antibodies of human and rabbit origin.
The production of actin antisera in rabbits was described and their reaction in various immunological tests were compared with the results obtained with human anti-actin sera (smooth muscle antibodies). Even if the sera may react with different antigenic determinants of actin as suggested by results in immunoprecipitation and blocking experiments, the rabbit and human sera can be used for studies of cells with equal or similar results. A prerequisite was that the rabbit anti-actin titres were high. The difficulty in obtaining strong anti-actin sera in rabbits was confirmed.
D000199:Actins / Q000276:immunology*; D000818:Animals; D000906:Antibodies; D003168:Complement Fixation Tests; D005347:Fibroblasts / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006386:Hemagglutination Tests; D006801:Humans; D005779:Immunodiffusion; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D009130:Muscle, Smooth / Q000276:immunology; D011817:Rabbits
D000199:Actins; D000906:Antibodies; D007074:Immunoglobulin G; D007075:Immunoglobulin M