Co-oxidation of carotenes requires one soybean lipoxygenase isoenzyme.
The type II lipoxygenase (optimum pH 6.5) from soybeans was purified and separated into two fractions either by chromatography on DEAE-Sephadex or by isoelectric focusing. In the presence of linoleic acid and oxygen both fractions co-oxidise canthaxanthine or beta-carotene as effectively as a combination of these fractions. Oxygenation of linoleic acid and co-oxidation of canthaxanthine by type II lipoxygenase is stimulated by 13-hydroperoxy-cis-9,trans-11-octadecadienoic acid but not by 13-hydroxy-cis-9,trans-11-octadecadienoic acid or 9-hydroperoxy-trans-10,cis-12-octadecadienoic acid.
D002338:Carotenoids / Q000031:analogs & derivatives / Q000378:metabolism*; D002850:Chromatography, Gel; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000302:isolation & purification*; D008084:Lipoxygenase / Q000302:isolation & purification*; D010944:Plants / Q000201:enzymology*; D013025:Glycine max; D014970:Xanthines / Q000378:metabolism
D007527:Isoenzymes; D014970:Xanthines; D002338:Carotenoids; D008084:Lipoxygenase