Laser differential light scattering bioassays for selected antitumor agents.
Bioassay methods for actinomycin D, 6-thioguanine, and adriamycin which utilize laser light scattering from suspensions of drug-sensitive bacteria have been developed. In 3 hours, serum and urine levels of the 3 drugs can be reproducibily measured. A sample volume of 0.1 ml may be assayed with a precision of +/- 10%. The linear response range for actinomycin D was 0.03 to 3.0 microgram/ml. For 6-thioguanine and adriamycin the linear responses range was 0.03 to 10.0 and 0.25 to 10.0 microgram/ml, respectively. Thetively. The present studies further confirm the general utility of differential light scattering as a rapid and inexpensive assay procedure of broad application.
D000970:Antineoplastic Agents / Q000032:analysis* / Q000494:pharmacology; D001681:Biological Assay / Q000295:instrumentation; D003609:Dactinomycin / Q000032:analysis; D004058:Diffusion; D004317:Doxorubicin / Q000032:analysis; D013293:Enterococcus faecalis / Q000187:drug effects; D007834:Lasers; D009391:Nephelometry and Turbidimetry; D013866:Thioguanine / Q000032:analysis
D000970:Antineoplastic Agents; D003609:Dactinomycin; D004317:Doxorubicin; D013866:Thioguanine