Ion-pair high-performance liquid chromatography: the use of low concentrations of long-chain alkylbenzyldimethylammonium chlorides for resolving anionic solutes.
The qualitative and quantitative determination of large anionic solutes, including the drug sodium cromoglycate, by an ion-pair high-performance liquid chromatographic technique using alkylbenzyldimethylammonium chlorides as pairing ions is described. The method provides a sensitive and selective assay for the solutes examined in both simple aqueous solutions and in complex biological fluids, and requires no pre-extraction step. Retention mechanisms are discussed.
D000838:Anions / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004205:Cromolyn Sodium / Q000652:urine; D006801:Humans; D000644:Quaternary Ammonium Compounds; D013501:Surface-Active Agents
D000838:Anions; D000644:Quaternary Ammonium Compounds; D013501:Surface-Active Agents; D004205:Cromolyn Sodium