Problems with particle-associated DNA polymerase assays in the diagnosis of plasma-suspended viruses.
The in vitro reaction results of virus-associated DNA polymerases for the demonstration of plasma-suspended particles of avian leukemia virus (AMV) and of hepatitis type B virus (HBV) were compared. AMV particles could be identified by the transcription of the templates poly mC(dG)12-18, poly rAT10, and poly d(AT) using standardized reaction mixtures. With comparable test conditions, no DNA polymerase activity was found in human plasma containing HBV. These findings and the results of a systematic study of factors influencing the polymerization assays are discussed.
D000818:Animals; D001354:Avian Leukosis Virus / Q000201:enzymology / Q000302:isolation & purification*; D002474:Cell-Free System; D002645:Chickens; D004256:DNA Polymerase I / Q000378:metabolism*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D006515:Hepatitis B virus / Q000201:enzymology / Q000302:isolation & purification*; D006801:Humans; D010949:Plasma / Q000382:microbiology*; D011119:Polynucleotides / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*; D013698:Templates, Genetic; D014158:Transcription, Genetic
D011119:Polynucleotides; D012194:RNA-Directed DNA Polymerase; D004256:DNA Polymerase I; D004259:DNA-Directed DNA Polymerase