Isolation and characterization of the nuclear matrix from the male Xenopus laevis following estrogen administration: kinetics of [3H] uridine incorporation.
At various times following estrogen administration, the nuclear matrix was isolated from the liver of male Xenopus laevis by sucrose gradient centrifugation of nuclei treated with a high-salt buffer and DNase I in the presence of a proteolytic inhibitor (PMSC--phenylmethyl sulfonyl chloride). Electron micrographs of the nuclear matrix demonstrate a sponge-like network attached to a well-defined inner envelope with a ribosome-free outer envelope. Chemical analyses show that the HSB-DNase-treated nuclei consist of 16% DNA, 2% RNA, and 82% protein, a composition that is consistent with that of nuclear matrices isolated from other species. The specific activity of the matrix-associated RNA following estrogen treatment appears to be maximally enhanced after 5 h and decreases until approximately 12 h, when the activity begins to increase again.
D000818:Animals; D002467:Cell Nucleus / Q000032:analysis*; D004958:Estradiol / Q000494:pharmacology*; D008099:Liver / Q000032:analysis / Q000648:ultrastructure*; D008297:Male; D008854:Microscopy, Electron; D009698:Nucleoproteins / Q000302:isolation & purification*; D012313:RNA / Q000378:metabolism; D012261:Ribonucleoproteins / Q000032:analysis / Q000302:isolation & purification*; D014529:Uridine / Q000378:metabolism; D014981:Xenopus
D009698:Nucleoproteins; D012261:Ribonucleoproteins; D004958:Estradiol; D012313:RNA; D014529:Uridine