[Methods to prepare histological microautoradiographs for evaluation by automatic image analysis (author's transl)].
A prerequisite for automatic image analysis of histoautoradiographs is the arrangement of the silver grains within the same plane of focus. This can be obtained by semi thin sections of 1 micrometer in thickness. A sufficient contrast for the determination of the nuclear areas and the overlying silver grains at the same time can be reached by staining the preparations with Kernechtrot or Feulgen nucleal reaction. If a higher image contrast is desired sections measuring 2 micrometer or more in thickness may be subjected to the autoradiographic procedure without removing the resin.
D000818:Animals; D001345:Autoradiography; D002467:Cell Nucleus / Q000648:ultrastructure*; D003201:Computers; D003584:Cytological Techniques; D005552:Forelimb; D012038:Regeneration; D013194:Staining and Labeling; D014319:Triturus
Methoden zur Herstellung von histologischen Mikroautoradiogrammen für die automatische Bildanalyse.