title: [Plasma estrogens in Pleurodeles waltlii. Comparison between diploid and triploid females].
abstract: Estrogens have been examined in the plasma of diploid and triploid newts Pleurodeles waltlii. Estradiol-17 beta (E2) and estrone (E1) were determined by radioimmunoassay, before and after enzymatic hydrolysis of the conjugates. Total (t) and unconjugated (u) E2 levels were positively correlated (E2u = 0.478 35 E2t + 0.579 98; r = 0.883), but no correlation was detected between E1 levels. No statistical difference was found for the estrogen levels between the different experimental lots of diploid newts (E2t = 7.5 +/- 0.37 ng/ml, E2u = 4.3 +/- 0.20 ng/ml, E1t = 2.19 +/- 0.08 ng/ml, E1t = 0.41 +/- 0.02 ng/ml) but every estrogen level was lower in the triploid group (E2t = 1.8 +/- 0.60, E2u = 1.0 +/- 0.18, E1t = 1.4 +/- 0.13, E1u = 0.3 +/- 0.04 ng/ml). This difference is discussed in relation to lower fertility of the triploid females.
mesh_terms: D000818:Animals; D004171:Diploidy; D004958:Estradiol / Q000097:blood; D004967:Estrogens / Q000097:blood*; D004970:Estrone / Q000097:blood; D005260:Female; D011123:Polyploidy; D012455:Salamandridae / Q000097:blood* / Q000235:genetics; D014562:Caudata / Q000097:blood*
chemical_list: D004967:Estrogens; D004970:Estrone; D004958:Estradiol
vernacular_title: Estrogènes plasmatiques chez Pleurodeles waltlii. Comparaison entre les femelles diploïdes et les femelles triploïdes.