Cytotoxic T cell responses to a syngeneic tumour: conditions for primary activation in vitro.
Primary cytotoxic responses of DBA/2 lymph node cells to a syngeneic tumour (the mastocytoma P815) have been generated in vitro. The development of these responses is dependent on the addition of a soluble factor (CSCS) which is produced by concanavalin A-activated spleen cells. The response is mediated by T lymphocytes, can be detected at low effector to target cell ratios and is directed against P815 tumour-associated antigens.
D000818:Animals; D003208:Concanavalin A / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic; D066298:In Vitro Techniques; D008198:Lymph Nodes / Q000166:cytology; D012515:Mast-Cell Sarcoma / Q000276:immunology*; D051379:Mice; D008811:Mice, Inbred DBA / Q000276:immunology; D012513:Sarcoma, Experimental / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D013601:T-Lymphocytes / Q000276:immunology*
D003208:Concanavalin A