Effect of 6-(p-hydroxyphenylazo)-uracil on the homologous and heterologous transduction processes in Bacillus subtilis.
We have studied the effect of 6-(p-hydroxyphenylazo)-uracil on the recombination processes that operate in the homologous and heterologous transduction mediated by PBS1 and SP10 phages of Bacillus subtilis. The results obtained demonstrate that the process of heterologous genetic exchange is sensitive to this compound, whereas the homologous process is not. The present data, along with those of our previous work (U. Canosi, A. G. Siccardi, A. Falaschi, and G. Mazza, J. Bacteriol. 126:108--121, 1976), suggest that the DNA polymerase III is involved in the recombination process that operates in transformation and heterologous transduction, whereas homologous transduction follows a partially independent pathway not involving this protein.
D001412:Bacillus subtilis / Q000187:drug effects / Q000235:genetics*; D001435:Bacteriophages / Q000235:genetics; D004258:DNA Polymerase III / Q000502:physiology; D006906:Hydroxyphenylazouracil / Q000494:pharmacology*; D014161:Transduction, Genetic / Q000187:drug effects*; D014498:Uracil / Q000031:analogs & derivatives*
D006906:Hydroxyphenylazouracil; D014498:Uracil; D004258:DNA Polymerase III