Rapid, sensitive and specific electron capture-gas chromatographic method for the quantitation of 6-chloro-2-(1-piperazinyl) pyrazine in biological fluids.
A highly specific and sensitive gas chromatographic method for the determination of 6-chloro-2-(1-piperazinyl)pyrazine (MK-212), a central serotonin-like agent, in biological fluids is described. MK-212 and a related internal standard are extracted into benzene from an alkaline solution, back-extracted into acid and then re-extracted into benzene at an alkaline pH. The amines are converted to the trifluoroacetyl derivatives (characterized by gas-liquid chromatography-mass spectrometry), chromatographed and detected with a 63Ni electron capture detector. The sensitivity of the method is such that 10 ng of drug can be measured per aliquot of biological fluid. The precision and accuracy of the method are well within acceptable limits. Specificity of analysis was established by gas-liquid chromatography-mass spectrometry techniques.
D000818:Animals; D002849:Chromatography, Gas; D004285:Dogs; D000882:Haplorhini; D008253:Macaca mulatta; D008297:Male; D013058:Mass Spectrometry; D008722:Methods; D010879:Piperazines / Q000032:analysis*; D011719:Pyrazines / Q000032:analysis*; D051381:Rats; D011985:Receptors, Serotonin / Q000187:drug effects*
D010879:Piperazines; D011719:Pyrazines; D011985:Receptors, Serotonin; C014896:6-chloro-2-(1-piperazinyl)pyrazine