[Extraction of plasma lipids preserving antigenic properties of proteins and allowing quantitation of gangliosides by neuraminic acid determination (author's transl)].
A technique described by Cham and Knowles allows the extraction of plasma phospholipides, triglycerides, cholesterol and free fatty acids without denaturing the proteins. The method has been slightly modified by us. Under our conditions, the yield of lipid extraction is comparable to the method of Folch. It has the advantage of not extracting mineral phosphorous and blood glucose. Thus gangliosides can be also easily determined. We confirmed by immunoelectrophoresis that the antigenic properties of plasmatic proteins remain after lipid extraction.
D001798:Blood Proteins / Q000276:immunology*; D002784:Cholesterol / Q000097:blood; D000939:Epitopes; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005732:Gangliosides / Q000097:blood; D006801:Humans; D007122:Immunoelectrophoresis; D008055:Lipids / Q000097:blood*; D008722:Methods; D009438:Neuraminic Acids / Q000097:blood; D010743:Phospholipids / Q000097:blood; D014280:Triglycerides / Q000097:blood
D001798:Blood Proteins; D000939:Epitopes; D005230:Fatty Acids, Nonesterified; D005732:Gangliosides; D008055:Lipids; D009438:Neuraminic Acids; D010743:Phospholipids; D014280:Triglycerides; D002784:Cholesterol
Méthode d'extraction des lipides plasmatiques conservant les propriétés antigéniques de protéines et permettant le dosage de l'acide sialique des gangliosides.