Solid-phase radioimmunoassay for hepatitis Be antigen (HBeAg).
A solid-phase radioimmunoassay was developed for the detection of HBeAg and anti-HBe in sera or serum fractions. HBe/sAg positive sera, partially purified HBeAg, partially purified HBsAg, and HBe/sAg negative sera were polymerized in polyacrylamide and compared for their ability to bind 125I-IgG (anti-HBe). Only gels containing HBeAg reacted specifically with the iodinated antibody. The specificity of the binding was confirmed by blocking and inhibition tests using anti-HBe, HBeAg, HBsAg, and negative control sera. The radioimmunoassay allows the specific and quantitative detection of HBeAg and anti-HBe even in the presence of detergents and high salt concentrations.
D001667:Binding, Competitive; D000939:Epitopes; D006510:Hepatitis B Antibodies / Q000032:analysis; D006511:Hepatitis B Antigens / Q000032:analysis*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007457:Iodine Radioisotopes; D011863:Radioimmunoassay / Q000379:methods*
D000939:Epitopes; D006510:Hepatitis B Antibodies; D006511:Hepatitis B Antigens; D007074:Immunoglobulin G; D007457:Iodine Radioisotopes