Assay of adenosine 3'-phosphate 5'-sulphatophosphate in hepatic tissues.
A fluorimetric assay, based on the ability of boiled hepatic extracts to support the sulphO-conjugation of harmol, was used to demonstrate and quantify PAdoPS (adenosine 3'-phosphate 5'-sulphatophosphate) present in liver. A stoicheiometric relationship was established between the sulphate conjugate formed and the 'active sulphate' utilized. Guinea-pig, rat, mouse and rabbit livers contain 3.3, 2.9, 0.8 and 0.5 mumol of PAdoPS/100 G wet wt. respectively.
D000249:Adenosine Monophosphate / Q000031:analogs & derivatives*; D000250:Adenosine Phosphosulfate / Q000032:analysis*; D000818:Animals; D004285:Dogs; D005260:Female; D006168:Guinea Pigs; D000882:Haplorhini; D008099:Liver / Q000032:analysis*; D008252:Macaca fascicularis; D008297:Male; D051379:Mice; D011817:Rabbits; D051381:Rats; D013045:Species Specificity; D013050:Spectrometry, Fluorescence
D000249:Adenosine Monophosphate; D000250:Adenosine Phosphosulfate