In vitro studies of the effect of metal ions, EDTA and their mixtures on kynurenine aminotransferase and kynurenine hydrolase.
In the present study use was made of the chelating ability of EDTA and the activating property of some metal ions Ca(II), Mg(II) or Mn(II) to counteract the inhibitory effect of Cu(II), Co(II), Pb(II) or Zn(II) ions on the B6-dependent kynurenine hydrolase and on kynurenine aminotransferase. These may be of help in studying the therapeutic trials in the treatment of metal poisoning. EDTA was able to counteract the inhibitory effect of Cu(II) or Co(II) on kynurenine aminotransferase and partially counteract the inhibitory effect of Cu(II), Co(II) on kynurenine aminotransferase and partially counteract the inhibitory effect of Cu(II), Co(II), Pb(II) or Zn(II) ions on kynurenine hydrolase. The difference in the response of the two B6-dependent enzymes to EDTA is attributed to the difference in the functional groups involved in the active site(s) of the two apoenzymes. Moreover, Mn(II), Ca(II) and Mg(II) ions have the ability to counteract some of the inhibitory effect of these metal ions.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D003300:Copper / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D006867:Hydrolases / Q000378:metabolism*; D066298:In Vitro Techniques; D007737:Kynurenine; D007854:Lead / Q000494:pharmacology; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D051379:Mice; D011773:Pyruvates; D014131:Trace Elements / Q000494:pharmacology*; D000637:Transaminases / Q000378:metabolism*; D015032:Zinc / Q000494:pharmacology
D011773:Pyruvates; D014131:Trace Elements; D007854:Lead; D007737:Kynurenine; D008345:Manganese; D003300:Copper; D004492:Edetic Acid; D000637:Transaminases; D006867:Hydrolases; D008274:Magnesium; D015032:Zinc; D002118:Calcium