Increase of liver microsomal benzo(a)pyrene monooxygenase activity by subsequent glucuronidation.
Benzo(a)pyrene monooxygenase activity in rat and guinea pig liver microsomes is markedly stimulated when UDP-glucuronic acid is present in the assay. This effect is further enhanced by addition of UDP-N-acetylglucosamine, an allosteric activator of UDP-glucuronyltransferase. The results suggest a coupling between microsomal monooxygenase and UDP-glucuronyltransferase which may assist elimination of toxic compounds.
D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000378:metabolism*; D001579:Benzopyrene Hydroxylase / Q000378:metabolism*; D005965:Glucuronates / Q000378:metabolism*; D014453:Glucuronosyltransferase / Q000378:metabolism; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D051381:Rats
D005965:Glucuronates; D001579:Benzopyrene Hydroxylase; D001189:Aryl Hydrocarbon Hydroxylases; D014453:Glucuronosyltransferase