Biosynthesis of exopolysaccharide by Pseudomonas aeruginosa.
In batch cultures of Pseudomonas aeruginosa, the maximum rate of exopolysaccharide synthesis occurred during exponential growth. In nitrogen-limited continuous culture, the specific rate of exopolysaccharide synthesis increased from 0.27 g g of cell-1 h-1 at a dilution rate (D) of 0.05 h-1 to 0.44 g g of cells h-1 at D=0.1 H-1. The yield of exopolysaccharide on the basis of glucose used was in the range of 56 to 64%. Exopolysaccharide was also synthesized in carbon-limited cultures at 0.19 g g of cell-1 h-1 at D=0.05 h-1 in a 33% yield. Nonmucoid variants appeared after seven generations in continuous culture and rapidly increased in proportion to the total number of organisms present.
D005947:Glucose / Q000378:metabolism; D007700:Kinetics; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D011550:Pseudomonas aeruginosa / Q000254:growth & development / Q000378:metabolism*
D011135:Polysaccharides, Bacterial; D005947:Glucose