title: Transformation of Pseudomonas aeruginosa strain PAO with bacteriophage and plasmid DNA.
abstract: A procedure has been developed which allows transformation of P. aeruginosa strain PAO with plasmid and bacteriophage DNA at a frequency of 10(-6) per recipient cell. The method is similar in outline to that developed for Escherichia coli. It involves growing the recipient cells to 3-5 x 10(8) per ml in nutrient broth, washing the cells with 0.1 M MgCl2, resuspending in 0.175 M CaCl2 for 20 min, exposing to DNA for 1 h and then heat pulsing at 42 degrees C for 1 min. Some plasmid markers are expressed immediately, whereas others require time for phenotypic expression.
mesh_terms: D001435:Bacteriophages / Q000235:genetics*; D002453:Cell Cycle; D004279:DNA, Viral / Q000235:genetics; D008722:Methods; D010957:Plasmids; D011550:Pseudomonas aeruginosa / Q000235:genetics*; D014169:Transformation, Bacterial; D014170:Transformation, Genetic
chemical_list: D004279:DNA, Viral