Antibody binding by native and denatured myosin and paramyosin.
By the techniques of immunodiffusion and fluorescent immunohistochemistry we show that antibodies to both the native and the SDS-denatured forms of the proteins, paramyosin and myosin, react with the native, SDS-denatured and glutaraldehyde-fixed forms of their respective antigens. Anti-denatured myosin also binds to both native and denatured forms of the proteolytic subfragments of myosin: globular subfragment-1 and alpha-helical LMM. Anti-native myosin, on the other hand, while able to bind to both native and denatured LMM or rod and to native and glutaraldehyde-fixed S-1, does not bind to SDS-denatured S-1.
D000818:Animals; D000906:Antibodies; D005455:Fluorescent Antibody Technique; D005779:Immunodiffusion; D009132:Muscles / Q000032:analysis; D009218:Myosins / Q000032:analysis*; D011489:Protein Denaturation; D011817:Rabbits; D014335:Tropomyosin / Q000032:analysis*
D000906:Antibodies; D014335:Tropomyosin; D009218:Myosins