A protein that preferentially binds Drosophila satellite DNA.
Using a nitrocellulose filter binding assay, we have detected and partially purified a protein from embryos of Drosophila melanogaster that preferentially binds to a highly repeated satellite DNA of the same species. Formation of the satellite DNA-protein complex requires physiological conditions of salt and temperature, but once formed, the complex is stable in high salt (1 M NaCl) or at low temperature. Optimal formation of the specific complex also requires the satellite DNA to be in a supertwisted conformation. The protein interacts with a limited region within the 359-base-pair repeated sequence of the satellite DNA.
D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000302:isolation & purification*; D004247:DNA / Q000378:metabolism*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004274:DNA, Recombinant; D004276:DNA, Satellite / Q000378:metabolism*; D004278:DNA, Superhelical / Q000378:metabolism; D004331:Drosophila melanogaster / Q000196:embryology / Q000235:genetics*; D009994:Osmolar Concentration; D010957:Plasmids; D013696:Temperature
D002352:Carrier Proteins; D004274:DNA, Recombinant; D004276:DNA, Satellite; D004278:DNA, Superhelical; D004247:DNA; D004262:DNA Restriction Enzymes