Cloned seventeen-nucleotide-long synthetic lactose operator is biologically active.
A 17-nucleotide-long synthetic DNA molecule constituting the minimal recognition sequence of the lactose operator has been cloned in E. coli using the vehicle pBR313 and a synthetic HindIII adaptor. The clones containing the lac-pBR313 hybrid DNA constitutively produced beta-galactosidase. The level of beta-galactosidase was high and comparable to that obtained in cells carrying a 21-nucleotide-long synthetic lac operator on pMB9 plasmid or cells carrying a natural lac operator on pOP203-1 plasmid.
D004247:DNA / Q000235:genetics*; D004269:DNA, Bacterial / Q000138:chemical synthesis; D004274:DNA, Recombinant / Q000235:genetics*; D004926:Escherichia coli; D005696:Galactosidases / Q000235:genetics*; D005796:Genes; D005815:Genetic Code; D007785:Lactose / Q000235:genetics; D009876:Operon; D001616:beta-Galactosidase / Q000235:genetics* / Q000378:metabolism
D004269:DNA, Bacterial; D004274:DNA, Recombinant; D004247:DNA; D005696:Galactosidases; D001616:beta-Galactosidase; D007785:Lactose