Photoelectron emission microscopy of biological tissue; the influence of glutaraldehyde and heavy or biligrafin on the emission image of chicken liver in methacrylate.
The effect of various preparations on the emission image was studied taking into account the influence of UV wave length, section thickness and specimen support. By suitable selection of these parameters, images with sufficient contrast can be obtained of unstained specimens which are only fixed with glutaraldehyde. With glutaraldehyde fixation intravitally injected Biligrafin used as a stain in cholecystography is recognized in the emission image of liver tissue labeling the intravital transport path from the blood capillaries to the bile ducts. Hypotheses for the image forming process are discussed.
D000447:Aldehydes; D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D005404:Fixatives; D005976:Glutaral; D007089:Image Enhancement; D007458:Iodipamide; D008099:Liver / Q000196:embryology / Q000648:ultrastructure*; D008660:Metabolism; D008689:Methacrylates; D008854:Microscopy, Electron / Q000379:methods*; D014466:Ultraviolet Rays
D000447:Aldehydes; D005404:Fixatives; D008689:Methacrylates; D005976:Glutaral; D007458:Iodipamide