Complete stabilization of water-soluble hydrogenase from Rhodospirillum rubrum under air atmosphere with a high concentration of chloride ions.
Hydrogenase was easily solubilized from light-grown cells of R. rubrum with 10 mM Na ethylenediaminetetraacetate. The enzyme thus obtained was so stable that loss of its activity was undetectable during storage at room temperature for 6 months under air atmosphere, provided that NaCl, KCl or CsCl was present at greater than or equal to 0.7 M.
D000332:Aerobiosis; D002712:Chlorides / Q000494:pharmacology*; D004355:Drug Stability; D006859:Hydrogen; D007700:Kinetics; D008027:Light; D009994:Osmolar Concentration; D010088:Oxidoreductases / Q000378:metabolism*; D012247:Rhodospirillum rubrum / Q000201:enzymology*; D012965:Sodium Chloride / Q000494:pharmacology; D012995:Solubility
D002712:Chlorides; D012965:Sodium Chloride; D006859:Hydrogen; D010088:Oxidoreductases