The phosphoenolpyruvate : methyl-alpha-d-glucoside phosphotransferase system in Bacillus subtilis Marburg : kinetic studies of enzyme ii and evidence for a phosphoryl enzyme ii intermediate.
The Enzyme II complex catalyzing the phosphoryl transfer from P-HPr to sugar in the inducible methyl-alpha-D-glucoside : phosphotransferase system in Bacillus subtilis acts according to a ping-pong mechanism, implying a phosphorylated Enzyme II intermediate. This result is supported by the demonstration of a specific transphosphorylation between [14C] alphaMG and glucose-6-phosphate in the presence of an induced Enzyme II preparation.
D001412:Bacillus subtilis / Q000201:enzymology*; D005958:Glucosephosphates / Q000378:metabolism; D007700:Kinetics; D008757:Methylglucosides / Q000378:metabolism; D010728:Phosphoenolpyruvate; D010770:Phosphotransferases / Q000378:metabolism*
D005958:Glucosephosphates; D008757:Methylglucosides; D010728:Phosphoenolpyruvate; D010770:Phosphotransferases