Membrane-bound mitochondrial DNA: isolation, transcription and protein composition.
Mitochondrial membrane-bound DNA complex from bovine heart mitochondria lysed in the presence of Triton X-100 was isolated by differential centrifugation. The yield of "nucleoid" is about 30 microgram protein/mg mitochondrial protein. It contains about 3-5 microgram DNA/mg protein and varying amounts of RNA. The heart mitochondrial nucleoid actively synthesizes RNA. The nucleoid fraction contains about sixteen different proteins as evidenced by urea-SDS gel electrophoresis and about twenty-one proteins as evidenced by acid-urea gel electrophoresis. It appears that the nucleoid is attached to the inner membrane since it does contain cytochromes.
D000818:Animals; D002417:Cattle; D002458:Cell Fractionation; D003580:Cytochromes / Q000032:analysis; D004272:DNA, Mitochondrial / Q000302:isolation & purification / Q000378:metabolism*; D003852:Deoxyribonucleoproteins / Q000032:analysis*; D007425:Intracellular Membranes / Q000378:metabolism*; D008929:Mitochondria, Heart / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000378:metabolism*; D009698:Nucleoproteins / Q000032:analysis*; D051381:Rats; D013053:Spectrophotometry; D014158:Transcription, Genetic
D003580:Cytochromes; D004272:DNA, Mitochondrial; D003852:Deoxyribonucleoproteins; D009698:Nucleoproteins