[Comparison of the values of selected reagents for the diagnosis of swine brucellosis. I. Preparation and standardization of antiglobulin serum].
The purpose of these studies was to make and to evaluate serums (SAG) helpful to the carrying out of OAG in diagnosis of pig brucellosis. Immunological serums directed against globulin fraction of pig serum were produced on rabbits immunizing them with "Suiglobin" preparation or with own preparation--"purified globulins" obtained from pig serum. Królak immunization method was used and in parallel that of Ressang's for the sake of comparison. Obtained SAG was titrated in the quick and slow OAG according to Hajdu. It was shown that rabbit immunization according to Ressang gives better results than that of Królak's. With the help of the immunoelectrophoresis reaction, in all SAG, the presence of the antibodies precipitating subfractions IgG and IgM of pig serum was found. Moreover, it was shown that to determine the final, working dilution of SAG, its titration was necessary in the slow, sample variant of OAG according to Hajdu.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000096:biosynthesis; D002003:Brucella abortus / Q000276:immunology*; D002006:Brucellosis / Q000175:diagnosis / Q000662:veterinary*; D003298:Coombs Test / Q000662:veterinary*; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D007202:Indicators and Reagents / Q000592:standards; D011817:Rabbits; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis*
D000888:Antibodies, Anti-Idiotypic; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007202:Indicators and Reagents
Porówanie wartości wybranych odczynów w rozpoznawaniu brucelozy świń. I. sporzadzenie surowicy antyglobulinowej oraz jej standaryzacja.