title: Calcium-specific precipitation of dentin phosphoprotein: a new method of purification and the significance for the mechanism of calcification.
abstract: Calcium, but not magnesium or strontium ions, specifically induce the precipitation of dentin phosphoprotein, which is reversibly solubilized by EDTA. This finding is very useful in pruifying dentin phosphoprotein of either a free or matrix-bound type. Bovine dentin phosphoproteins, thus isolated, contain a minimal amount of contaminant protein, unlike previous preparations.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001665:Binding Sites; D002118:Calcium; D002417:Cattle; D011232:Chemical Precipitation; D002848:Chromatography, DEAE-Cellulose; D003804:Dentin / Q000032:analysis*; D004492:Edetic Acid; D010750:Phosphoproteins / Q000302:isolation & purification*
chemical_list: D000596:Amino Acids; D010750:Phosphoproteins; D004492:Edetic Acid; D002118:Calcium