Methylation of basic proteins in ribosomes from wild-type and thiostrepton-resistant strains of Bacillus megaterium and their electrophoretic analysis.
Ribosomes, radioactively labelled in vivo with both [1-14C]methionine and [methyl-3H]methionine, have been isolated from both wild-type and thiostrepton-resistant strains of Bacillus megaterium and their constituent proteins separated by two-dimensional gel electrophoresis. Ribosomes from the wild-type strain possess one basic protein that is extensively methylated. In contrast no such protein can be detected in ribosomes from the thiostrepton-resistant strain.
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001410:Bacillus megaterium / Q000032:analysis* / Q000187:drug effects; D002250:Carbon Radioisotopes; D004352:Drug Resistance, Microbial; D008715:Methionine / Q000378:metabolism; D008745:Methylation; D012269:Ribosomal Proteins / Q000032:analysis*; D012270:Ribosomes / Q000032:analysis*; D013045:Species Specificity; D013883:Thiostrepton / Q000494:pharmacology*; D014316:Tritium
D000900:Anti-Bacterial Agents; D002250:Carbon Radioisotopes; D012269:Ribosomal Proteins; D014316:Tritium; D008715:Methionine; D013883:Thiostrepton