The errors in assembly of MuLV in interferon treated cells.
Interferon treatment of JLSV-6 cells chronically infected with Rauscher MuLV leads to the formation of noninfectious particles (interferon virions) containing the structural proteins of env and gag genes as well as additional viral polypeptides. In the control virions the major glycoprotein detected is gp71, interferon virions contain in addition to gp71 and 85k dalton (gp85) glucosamine-containing, fucose-deficient glycoprotein which is recognized by antiserum to MuLV but not by the gp71 antiserum. The surface iodination of the intact virions indicates that both gp71 and gp85 are the major components of the external virions envelope. However, unlike the control virions in which gp71 associates with p15E (gp90), the gp71-p15E complex was not detected in interferon virions. The analysis of the iodinated proteins of the disrupted interferon virions revealed the presence of 85k and 65k dalton polypeptides preciptable with antiserum against MuLV, which are not present in the control virions. The difference in the polypeptide pattern of virions produced in the presence of interferon does not seem to be a consequence of the slowdown in the synthesis of viral proteins or their processing in the interferon-treated cells. Both the structural proteins of env and gag genes seem to be synthesized and processed at a comparable rate in the interferon-treated and -untreated cells. These results indicate an alteration of virus assembly in the presence of interferon.
D000818:Animals; D001842:Bone and Bones; D002460:Cell Line; D000939:Epitopes; D006023:Glycoproteins / Q000096:biosynthesis; D007372:Interferons / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D011925:Rauscher Virus / Q000187:drug effects / Q000378:metabolism*; D014764:Viral Proteins / Q000096:biosynthesis* / Q000276:immunology; D014779:Virus Replication / Q000187:drug effects
D000939:Epitopes; D006023:Glycoproteins; D014764:Viral Proteins; D007372:Interferons