title: Induction of sex-linked recessive lethals and autosomal translocations by beta-propiolactone in Drosophila: influence of the route of administration on mutagenic activity.
abstract: Beta-propiolactone (BPL) was tested for the induction of sex-linked recessive lethals and autosomal translocations in Drosophila melanogaster. The compound was administered to adult males either by oral application or by abdominal injection. When injected, BPL was a potent inducer of sex-linked recessive lethals. When BPL was given by feeding, its mutagenic activity was detectable only when the flies were starved and when the BPL-containing solutions were renewed several times. Nevertheless, the recessive-lethal frequency was one order of magnitude higher with injection. This difference in effects is attributed to (1) rapid decomposition of the compound in aqueous feeding solutions, and to (2) rapid degradation in vivo which restricts the activity of BPL mainly to the site of application. These data are compared with other studies in which both routes of application were applied. BPL induced translocations in stored spermatozoa when injected, but not when fed. This finding seems a logical consequence of (1) the difference in effectiveness of the two routes of application for BPL, and (2) the existence of different LECs for mutation induction (recessive lethals) and for chromosome breakage (translocations). In Drosophila, the breakage capacity of BPL was one order of magnitude lower than that of MMS, when a comparison was made on the basis of equal recessive-lethal frequencies.
mesh_terms: D000284:Administration, Oral; D000818:Animals; D004331:Drosophila melanogaster / Q000235:genetics; D004353:Drug Evaluation, Preclinical; D005804:Genes, Lethal; D005808:Genes, Recessive; D008040:Genetic Linkage; D007274:Injections, Intraperitoneal; D007783:Lactones / Q000494:pharmacology*; D008297:Male; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D009153:Mutagens; D011420:Propiolactone / Q000008:administration & dosage / Q000494:pharmacology*; D012730:Sex Chromosomes / Q000187:drug effects*; D014178:Translocation, Genetic
chemical_list: D007783:Lactones; D009153:Mutagens; D011420:Propiolactone; D008741:Methyl Methanesulfonate