title: Occurrence of squalene and sterols in Cellulomonas dehydrogenans (Arnaudi 1942) comb. nov. Hester 1971.
abstract: The neutral lipid fraction of the photochromogenic, coryneform bacterium Cellulomonas dehydrogenans (Arnaudi 1942) comb. nov. contains the sterol precursor squalene and at least two sterols, cholesterol and beta-sitosterol. The compounds were characterized by mass spectrometry and combination gas-liquid chromatography--mass spectrometry. De novo sterol biosynthetic ability was shown from incorporation of 14C from D-[U-14C]glucose into squalene and the sterol fraction. The squalene concentration approximated 0.002 to 0.005% of the total dry cell weight, and the sterols approximated 0.03 to 0.05%.
mesh_terms: D000192:Actinomycetales / Q000032:analysis* / Q000378:metabolism; D002784:Cholesterol / Q000032:analysis* / Q000096:biosynthesis; D002849:Chromatography, Gas; D013058:Mass Spectrometry; D012855:Sitosterols / Q000032:analysis* / Q000096:biosynthesis; D013185:Squalene / Q000032:analysis*
chemical_list: D012855:Sitosterols; D013185:Squalene; D002784:Cholesterol