title: Regional preference of insertion of Tn501 and Tn802 into RP1 and its derivatives.
abstract: The sites of insertion of Tn501 into RP1 and into derivatives of this plasmid that either lack the Tn801 (TnA) element or contain it in a different location have been determined. Similarly, the sites of insertion of Tn802 into a derivative of RP1 that lacks the Tn801 element and into recombinants of this plasmid with Tn501 were determined. 'Hot spots' for insertion were observed with both transposons; but it is clear that a particular DNA sequence is not sufficient to define a 'hot spot', since a particular region does contain many insertions when present in one plasmid but does not do so when part of another.
mesh_terms: D001483:Base Sequence; D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial / Q000235:genetics*; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D011550:Pseudomonas aeruginosa / Q000235:genetics; D011815:R Factors; D011995:Recombination, Genetic
chemical_list: D004269:DNA, Bacterial