[Use of a new radioenzymatic test in urticaria and drug allergy].
The Authors report preliminary data obtained with the histamine release test, evaluated with a radioenzymatic technique, in patients with a symptomatology highly indicative for penicillin allergy (21 cases) and in patients with urticarial forms (20 cases) or atopic dermatitis (5 cases). This technique, suitably modified, turned out very sensitive and able to detect histamine concentrations in the picogram range. The use of this test in type I immunoreactions, after Ig removal and fixation (IgE, IgG4) from basophils of atopic and normal subjects, pointed out its remarkable advantages (sensitivity, specificity, reliability) in comparison with other techniques. The results of our case-list, even if preliminary seem to be indicative for an extension of the trial to a wider number of patients both with allergies of pharmacological type and with urticarial forms or atopic dermatitis.
D001491:Basophils / Q000276:immunology; D003876:Dermatitis, Atopic / Q000175:diagnosis; D004342:Drug Hypersensitivity / Q000175:diagnosis*; D006636:Histamine Release; D006801:Humans; D007124:Immunoenzyme Techniques; D007073:Immunoglobulin E; D007074:Immunoglobulin G; D010406:Penicillins / Q000009:adverse effects; D014581:Urticaria / Q000175:diagnosis*
D007074:Immunoglobulin G; D010406:Penicillins; D007073:Immunoglobulin E
Impiego di un nuovo test radioenzimatico nelle forme orticariodi e nelle allergie da farmaci.