Nucleoli visualized by silver staining combined with a new RNA-specific fluorochrome.
A technique for distinguishing nucleolar components by light microscopy is described. Silver staining is used for localizing nuceolar-organizing regions. A new RNA-specific fluorochrome, 4''-6-bis(2'-imidazolinyl-4'-5'H)-2-phenyl-4'-phenoxyindole, visualizes the RNA-containing "partes fibrillares et granulares" of the nucleolus. Both staining procedures can be combined to analyze nucleolar structure and function by light microscopy.
D002466:Cell Nucleolus / Q000032:analysis / Q000648:ultrastructure*; D005456:Fluorescent Dyes; D006801:Humans; D008214:Lymphocytes / Q000648:ultrastructure; D009697:Nucleolus Organizer Region / Q000648:ultrastructure; D012313:RNA / Q000032:analysis*; D012834:Silver; D013194:Staining and Labeling / Q000379:methods*
D005456:Fluorescent Dyes; D012834:Silver; D012313:RNA