A millipore--blue dextran affinity binding system.
Blue dextran has been adsorbed to millipore filter discs in a linkage stable under a variety of conditions. These discs have been to bind lactic dehydrogenase, which may then be specifically eluted with NADH, one of its substrates. In contrast, another enzyme, alkaline phosphatase, not expected to bind to blue dextran, was indeed completely recovered in the filtrate.
D000469:Alkaline Phosphatase / Q000302:isolation & purification; D001665:Binding Sites; D003911:Dextrans; D007770:L-Lactate Dehydrogenase / Q000302:isolation & purification; D011506:Proteins / Q000302:isolation & purification*; D014462:Ultrafiltration / Q000295:instrumentation*
D003911:Dextrans; D011506:Proteins; C017080:blue dextran; D007770:L-Lactate Dehydrogenase; D000469:Alkaline Phosphatase