title: Iso-branched 2- and 3-hydroxy fatty acids as characteristic lipid constituents of some gliding bacteria.
abstract: The fatty acids present in the total hydrolysates of several gliding bacteria (Myxococcus fulvus, Stigmatella aurantiaca, Cytophaga johnsonae, Cytophaga sp. strain samoa and Flexibacter elegans) were analyzed by combined gas-liquid chromatography and mass spectrometry. In addition to 13-methyl-tetradecanoic acid, 15-methyl-hexadecanoic acid, hexadecanoic acid, and hexadecenoic acid, 2- and 3-hydroxy fatty acids comprised up to 50% of the total fatty acids. The majority was odd-numbered and iso-branched. Small amounts of even-numbered and unbranched fatty acids were also present. Whereas 2-hydroxy-15-methyl hexadecanoic acid was characteristic for myxobacteria, 2-hydroxy-13-methyl-tetradecanoic acid, 3-hydroxy-13-methyl-tetradecanoic acid, and 3-hydroxy-15-methyl-hexadecanoic acid were dominant in the Cytophaga-Flexibacter group.
mesh_terms: D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D003589:Cytophaga / Q000032:analysis*; D041964:Cytophagaceae / Q000032:analysis*; D003652:Decanoic Acids / Q000032:analysis*; D008055:Lipids / Q000032:analysis*; D009231:Myxococcales / Q000032:analysis*; D013045:Species Specificity
chemical_list: D003652:Decanoic Acids; D008055:Lipids