Structural components of sphingophosphonolipids from the ciliated protozoan, Tetrahymena pyriformis WH-14.
1. Two sphingophosphonolipids were isolated from the lipids of the ciliated protozoan, Tetrahymena pyriformis WH-14. They were ceramide N-methyl-2-aminoethylphosphonate (CMAEP) and ceramide 2-aminoethylphosphonate (CAEP), in yields of 0.05 mg/g and 1.74 mg/g dry cells, respectively. 2. Two chromatographically distinguishable CAEP species were found, a slow-moving major component and a minor component which moved faster; the slow-moving one contained primarily hydroxy fatty acids, while in the other one nonhydroxy fatty acids were predominant. However, their long-chain base constituents were similar. 3. The major fatty acids of CAEP were 2-hydroxy acids with carbon numbers of 16 to 19, which were almost exclusively iso-types. The fatty acids of CMAEP consisted mainly of palmitic, iso-octadecanoic, and 2-hydroxy iso-heptadecanoic acids. 4. The long-chain bases were dominated by C16, C17, and C19 iso-4-sphingenine homologs.
D000818:Animals; D002518:Ceramides / Q000032:analysis; D002849:Chromatography, Gas; D005227:Fatty Acids / Q000032:analysis; D013058:Mass Spectrometry; D063065:Organophosphonates / Q000032:analysis; D013107:Sphingolipids / Q000032:analysis*; D013769:Tetrahymena pyriformis / Q000032:analysis*
D002518:Ceramides; D005227:Fatty Acids; D063065:Organophosphonates; D013107:Sphingolipids