The preparation of 59Fe-labelled transferrin of high radioactivity for ferrokinetic studies on small laboratory animals.
A modified method of Cavill for the separation of the complex of isotopically labelled iron and transferrin from unbound iron residues is described. The method employing an ion exchanger permits to obtain preparations with a high radioactivity per volume unit of solution, which is necessary for carrying out ferrokinetic studies on small laboratory animals.
D000818:Animals; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange / Q000379:methods*; D007501:Iron / Q000378:metabolism*; D007504:Iron Radioisotopes; D051379:Mice; D008810:Mice, Inbred C57BL; D014168:Transferrin / Q000302:isolation & purification*
D007504:Iron Radioisotopes; D014168:Transferrin; D007501:Iron