Diffusion in gel-enzyme linked immunosorbent assay (DIG-ELISA): a simple method for quantitation of class-specific antibodies.
A new method for quantifying class-specific antibodies is presented. The method has been named Diffusion-In-Gel-Enzyme-Linked-ImmunoSorbentAssay (DIG-ELISA), and is briefly as follows. Antiserum ia allowed to diffuse from wells in a gel layered over an antigen-coated plastic surface. The gel is then removed and the preparation is incubated with enzyme-conjugated anti-immunoglobulin. The enzyme is then visualised in situ by a colour reaction produced by pouring a substrate-containing gel over the plastic surface. Bovine serum albumin and rabbit-anti-BSA were used as a model system, and horseradish peroxidase or alkaline phosphatase as enzymes for visualization.
D000469:Alkaline Phosphatase; D000562:Amebiasis / Q000175:diagnosis; D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology; D000918:Antibody Specificity; D002417:Cattle; D004306:Dose-Response Relationship, Immunologic; D004797:Enzyme-Linked Immunosorbent Assay; D006041:Goats; D006735:Horseradish Peroxidase; D006801:Humans; D005779:Immunodiffusion; D008070:Lipopolysaccharides / Q000276:immunology; D011817:Rabbits; D012217:Rheumatoid Factor; D012552:Schistosomiasis / Q000175:diagnosis; D013954:Thyroglobulin / Q000276:immunology; D013966:Thyroiditis / Q000175:diagnosis
D000888:Antibodies, Anti-Idiotypic; D008070:Lipopolysaccharides; D012217:Rheumatoid Factor; D013954:Thyroglobulin; D006735:Horseradish Peroxidase; D000469:Alkaline Phosphatase