title: Deoxyribonucleoside-requiring mutants of Bacillus subtilis.
abstract: A number of deoxyribonucleoside-requiring mutants (dns) of Bacillus subtilis were isolated and their growth characteristics and ribonucleotide reductase activities were compared with those of the wild type and of a dna mutant (tsA13). Both tsA13 and dns mutants required the presence of a mixture of deoxyribonucleosides for growth at 45 degrees C but not at 25 degrees C. All the mutant strains tested contained ribonucleotide reductase activity which showed heat sensitivity similar to that of the enzyme from a wild-type strain. The reductase in B. subtilis seemed to reduce ribonucleoside triphosphates in a similar manner to the enzyme in Lactobacillus leichmannii.
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D004269:DNA, Bacterial / Q000096:biosynthesis; D003853:Deoxyribonucleosides / Q000378:metabolism*; D006918:Hydroxyurea / Q000494:pharmacology; D009154:Mutation; D010641:Phenotype; D012264:Ribonucleotide Reductases / Q000378:metabolism; D013696:Temperature
chemical_list: D004269:DNA, Bacterial; D003853:Deoxyribonucleosides; D012264:Ribonucleotide Reductases; D006918:Hydroxyurea