[Further study of the nature and characteristics of the rosette formation phenomenon by a modified Jerne's method].
Experiments were conducted on 105 mongrel female albino mice weighing 18--22 g (intact, irradiated in a dose of 700 r with gamma-rays 60Co or immunized with SRBC) and on intact hybrids F1, CBAxC57BL. Phase contract microscopy showed autoimmune hemolysis plaques to be large or irregular and to posses a girdle made up of deformed and lysed erythrocytes and to contain stroma "ghosts" of the latter around the central cell. Plaques obtained in hypertensive medium were always round and the bordering girdle consisted of intact erythrocytes, whereas the plaque filed was homogeneous or contained granules. The concentration of sodium, potassium, calcium, and magnesium atoms in the suspension fluid of the preparations failed to exceed their content in the cells (as demonstrated by Jerne's method modified by the authors on a mixture of spleen cells with autologous blood). Addition of antiglobulin serum into the preparation on a slide in a 1:5 dilution suppressed the formation of autoimmune plaques, and in a dilution of 1:50--increased it considerably.
D000818:Animals; D001323:Autoantibodies / Q000032:analysis; D005260:Female; D005720:Gamma Rays; D006462:Hemolytic Plaque Technique; D007114:Immunization; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008858:Microscopy, Phase-Contrast / Q000379:methods; D010284:Paratyphoid Fever / Q000276:immunology; D012397:Rosette Formation / Q000379:methods*; D013053:Spectrophotometry / Q000379:methods; D013054:Spectrophotometry, Atomic / Q000379:methods
D001323:Autoantibodies
Dal'neĭshee izuchenie prirody i osobennosteĭ iavleniia bliashkoobrazovaniia pri modifitsirovannom metode Erne.