Combination of a viability test and a quantification method for Schistosoma haematobium eggs (filtration--trypan blue-staining-technique).
The efficiency of the polycarbamate membrane filtration technique for detecting Schistosoma haematobium eggs in urine has been increased by using a pore size of 14 micrometers diameter and the suction of a water jet pump. The technique permits filtration of several 1000 ml of urine in one test. Eggs concentrations of 1 egg in more than 1000 ml of urine were detected. Viability was assess after filtration by staining with trypan blue. Preliminary data of 118 Schistosoma haematobium-positive urine samples of 32 patients suggest that the filtration trypan blue-staining-technique is of high validity in light infection with scanty egg-output.
D005374:Filtration / Q000379:methods; D006801:Humans; D007814:Larva / Q000254:growth & development; D010270:Parasite Egg Count / Q000379:methods*; D012548:Schistosoma haematobium / Q000254:growth & development; D012552:Schistosomiasis / Q000175:diagnosis* / Q000188:drug therapy / Q000652:urine; D013194:Staining and Labeling; D014236:Trichlorfon / Q000627:therapeutic use
D014236:Trichlorfon