[Analysis of the biological effect of city smog extract. III. Comparative investigations on the effect of city smog extracts on cell replication and DNA-synthesis of kidney cells in vitro from the primate Cercopithecus aethiops (author's transl)].
We analysed the cytotoxic effect of city smog extracts from a heavy industrialized area using kidney cell cultures from the primate Cercopithecus aethiops.--Using logarithmically growing cell cultures we determined cell replication and the rate of DNA-synthesis after incorporation of 3H-thymidine.--In presence of city smog extracts we found a dose dependent reduction of cell replication and of DNA-synthesis. In presence of high concentrations (BP-aquivalent 0.25-0.5 microgram/ml) of city smog extract we found no increase in cell number over a period of 72 h. Under the same conditions hardly any DNA-synthesis was detected.--In presence of middle and low concentrations of city smog extract a dose- and time-dependent increase in cell number and rate of DNA-synthesis was detected.
D000818:Animals; D001580:Benzopyrenes / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects*; D002521:Cercopithecus; D046508:Culture Techniques; D004247:DNA / Q000096:biosynthesis*; D005860:Germany, West; D000882:Haplorhini; D007668:Kidney; D012905:Smog
D001580:Benzopyrenes; D012905:Smog; D004247:DNA
Untersuchung der biologischen Wirkung von atmosphärischen Feinstaubextrakten. III. Vergleichende Untersuchungen über die Wirkung von Feinstaubextrakten auf die Zellvermehrung und DNS-synthese von Nierenzellen des Primaten Cercopithecus aethiops in vitro.