Effects of Ca, Mg, and EDTA on creatine kinase activity in cerebrospinal fluid.
For one to obtain a precise estimate of creatine kinase (CK) activity in cerebrospinal fluid, the sample fraction is increased by about 10-fold over that used for serum. This increases the concentration of interfering substances, Ca being especially important. Therefore, the relationship between Ca, Mg, and EDTA was examined. Enzyme activity was maximal with 15 mmol of Mg per liter in the presence of 3 mmol of EDTA per liter, otherwise according to the (Scandinavian) recommended conditions for determination of CK activity in serum. These modifications increased the activity of CK by 35% for CK-MM and by 60% for CK-BB. Counteraction of Ca-induced inhibition was the main reason to this increase. We describe a practical and sensitive method for determining CK in cerebrospinal fluid.
D002021:Buffers; D002118:Calcium / Q000494:pharmacology*; D003402:Creatine Kinase / Q000134:cerebrospinal fluid*; D004492:Edetic Acid / Q000494:pharmacology*; D004789:Enzyme Activation; D006801:Humans; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology*
D002021:Buffers; D004492:Edetic Acid; D003402:Creatine Kinase; D008274:Magnesium; D002118:Calcium