Rauscher leukemia virus-induced tumor antigens: complete separation from gp70, p30 and H-2.
The possibility that some or all of the viral proteins, gp70, p30, and the histocompatibility antigen, H-2, function as the tumor-specific transplantation antigen (TSTA) of the R-MuLV-induced leukemia, RBL-5, and also in the secondary in vitro induction of cytotoxic T lymphocytes (CTL), was investigated. The antigen was obtained by isolating the plasma membranes of RBL-5 cells and solubilizing with sodium deoxycholate (DOC) followed by gel filtration chromatography. A fraction containing excellent tumor-rejection activity but low amounts of gp70, p30 and H-2 was chromatographed on goat anti-gp 70 goat anti-p 30 and sheep anti-H-2b immunoaffinity columns. The data obtained indicate that gp 70, p 30 or H-2 do not function as TSTA of RBL-5 leukemia, individually or as a complex. Similarly, the antigen responsible for the specific secondary induction of CTL in vitro is distinct from these three proteins.
D000818:Animals; D000951:Antigens, Neoplasm / Q000302:isolation & purification; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D003602:Cytotoxicity, Immunologic; D000939:Epitopes; D006183:H-2 Antigens; D006649:Histocompatibility Antigens; D007114:Immunization; D007136:Immunoglobulins / Q000032:analysis; D007942:Leukemia, Experimental / Q000276:immunology*; D051379:Mice; D011863:Radioimmunoassay; D011925:Rauscher Virus / Q000276:immunology*; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology; D014764:Viral Proteins / Q000032:analysis
D000951:Antigens, Neoplasm; D000939:Epitopes; D006183:H-2 Antigens; D006649:Histocompatibility Antigens; D007136:Immunoglobulins; D014764:Viral Proteins