A precursor of cytoplasmic actin in cultured Drosophila cells.
In continuous lines of cultured Drosophila cells two forms of cytoplasmic actin, designated II and III, are detected after a 25- to 120-min pulse label with [35S]methionine. However, only one of these, actin II, accumulates in the cell. With the use of a pulse-chase protocol and two-dimensional gel electrophoresis, it has been found that actin III is synthesized as a precursor of the more stable cytoplasmic actin II. The half-life of actin III was estimated to be approximately 50 min.
D000199:Actins / Q000096:biosynthesis*; D000818:Animals; D001345:Autoradiography; D002478:Cells, Cultured; D003593:Cytoplasm / Q000378:metabolism*; D004331:Drosophila melanogaster; D004586:Electrophoresis; D007700:Kinetics; D011498:Protein Precursors / Q000096:biosynthesis*
D000199:Actins; D011498:Protein Precursors