Comparative bactericidal activities of beta-lactam antibiotics determined in agar and broth media.
Comparative bactericidal activities were determined utilizing a relatively large number of test strains, in both agar and broth media, with special reference to the time of exposure of the bacteria to certain beta-lactam antibiotics. It was apparent that the activities increase with time. The concentrations producing a 99.9% kill with cephalothin for Escherichia coli, Klebsiella sp., and carbenicillin for Pseudomonas aeruginosa were higher in broth than in agar. In contrast, those of benzylpenicillin for alpha-streptococcus (non-enterococcal) were higher in agar than in broth. If the bactericidal concentrations with 3-hour or 6-hour exposure to antibiotics were used as the criterion, these concentrations of carbenicillin for P. aeruginosa, and benzylpenicillin for alpha-streptococcus were, in particular, unusually high compared with the conventionally determined bacteriostatic concentrations (MICs).
D000362:Agar; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D003470:Culture Media; D007709:Klebsiella / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D001618:beta-Lactamases / Q000378:metabolism; D047090:beta-Lactams / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D003470:Culture Media; D047090:beta-Lactams; D000362:Agar; D001618:beta-Lactamases