Fluorescence analysis of cells using a laser light source.
A quantitative microfluorometric instrument is described that employs a helium cadmium laser (442 nm) as the illumination source. The instrument consists of a double grating monochromator in front of a gallium arsenide photomultiplier that is interfaced with a desktop computer. The versatility of the instrument in making quantitative nucleic acid measurements on acridine orange and Feulgen-Schiff stained cells is demonstrated. The ploidy levels of several populations are easily determined, and the Feulgen fluorescent emissions are considerably greater than those obtained with a standard mercury lamp.
D000818:Animals; D002460:Cell Line; D003201:Computers; D003584:Cytological Techniques / Q000295:instrumentation*; D004247:DNA / Q000032:analysis; D005456:Fluorescent Dyes; D007834:Lasers; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*
D005456:Fluorescent Dyes; D004247:DNA