Genetic mechanism accounting for precise immunoglobulin domain deletion in a variant of MPC 11 myeloma cells.
A variant of the MPC 11 cell line, M 311, produces a short immunoglobulin heavy chain. When compared with the parental gamma 2b heavy chain, M 311 was found to have a carboxyl terminal deletion comprising the CH3 domain. The COOH-terminal cyanogen bromide (CNBr) cleavage fragment of M 311 is identical to a corresponding segment ofa parental heavy chain CNBr fragment, with the exception of a substitution of asparagine for lysine at the COOH-terminal residue. This observation enabled prediction of both the parental DNA sequence in this region and the genetic mechanism which generated the variant, a frameshift followed by premature termination. This hypothesis is supported by studies of the DNA sequence of the MPC 11 gamma 2b constant region gene.
D000818:Animals; D002460:Cell Line; D002872:Chromosome Deletion; D005796:Genes; D007074:Immunoglobulin G / Q000235:genetics*; D007142:Immunoglobulin gamma-Chains / Q000235:genetics; D046911:Macromolecular Substances; D008558:Melphalan / Q000494:pharmacology; D051379:Mice; D009154:Mutation; D009194:Myeloma Proteins / Q000235:genetics*; D009374:Neoplasms, Experimental / Q000235:genetics; D010443:Peptide Chain Termination, Translational; D010954:Plasmacytoma / Q000235:genetics
D007074:Immunoglobulin G; D007142:Immunoglobulin gamma-Chains; D046911:Macromolecular Substances; D009194:Myeloma Proteins; D008558:Melphalan