title: A simple method for the preparation of antisera specific for murine immunoglobulin heavy chains.
abstract: Rabbit antisera were prepared against mouse immunoglobulin mu and gamma chains by immunizing rabbits with their own erythrocytes coated with murine anti-rabbit erythrocyte immunoglobulin. In the preparation of these antisera, we exploited the observation that the primary immune response in C57BL/10ScSn (B10) mice is almost exclusively IgM, while hyperimmune antiserum of A/WySn (A) is mostly IgG. After appropriate absorption with thymocytes and rabbit erythrocytes (Rrbc) coated with B10 or A antiRrbc Ig, the sera were shown to be specific by immunoelectrophoresis, indirect immunofluorescent staining, and complement-mediated cytotoxicity. This technique is simple, requires almost no equipment, and a large quantity of specific antiserum can be prepared quite economically.
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D007122:Immunoelectrophoresis; D007143:Immunoglobulin Heavy Chains / Q000276:immunology*; D008722:Methods; D051379:Mice; D011817:Rabbits / Q000276:immunology
chemical_list: D007106:Immune Sera; D007143:Immunoglobulin Heavy Chains