Uptake of the tumor promoting agent 12-0-tetradecanoyl-phorbol-13-acetate by HeLa cells.
The uptake of 12-0-tetradecanoyl-phorbal-13-acetate (TPA) by HeLa cells, cells of a line responsive to the action of this tumor promoting agent, was studied. Our results indicate that most of the uptake is due to a physical nonsaturable process which is not grossly affected by inhibitors of energy metabolism or macromolecular synthesis. Uptake occurs mainly in membranous fractions of the cell, is largely reversible, and is temperature dependent. These results suggest that 3H-TPA uptake is largely due to partitioning of this lipophilic substance into the lipid phase of the cell membranes.
D000418:Albumins / Q000378:metabolism; D004492:Edetic Acid / Q000494:pharmacology; D006367:HeLa Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D010704:Phorbols / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism; D013696:Temperature; D013755:Tetradecanoylphorbol Acetate / Q000302:isolation & purification / Q000378:metabolism*; D014357:Trypsin / Q000494:pharmacology
D000418:Albumins; D010704:Phorbols; D004492:Edetic Acid; D014357:Trypsin; D013755:Tetradecanoylphorbol Acetate