Critical density for protein-protein monoconjugation on a polysaccharide matrix.
The galactose binding toxin (RCAII) from Ricinus communis was affinity-immobilised at varying densities on a polysaccharide matrix and reacted with glutaraldehyde. The critical density below which inter-molecular cross-links were not formed was determined. At this density RCAII was monoconjugated to lysozyme. This approach could serve as a prototype for enzyme-lectin and enzyme-antipolysaccharide antibody monoconjugation.
D003432:Cross-Linking Reagents; D003720:Densitometry; D005690:Galactose; D005976:Glutaral; D037102:Lectins; D009113:Muramidase; D011108:Polymers / Q000138:chemical synthesis; D011134:Polysaccharides; D011485:Protein Binding; D011506:Proteins; D014118:Toxins, Biological
D003432:Cross-Linking Reagents; D037102:Lectins; D011108:Polymers; D011134:Polysaccharides; D011506:Proteins; C024930:RCA II; D014118:Toxins, Biological; D009113:Muramidase; D005976:Glutaral; D005690:Galactose