title: Utilization of mismatched initiator termini by avian myeloblastosis virus DNA polymerase.
abstract: DNA synthesis by avian myeloblastosis virus was studied using poly(C) as template and modified oligo(dG) as primer. The addition of one noncomplementary base to the 3'-end of the primer has no important effect on synthesis. The mispaired base is incorporated into the product and the apparent Km (for primer) and the V of the reaction remain unchanged. This confirms the absence of a 3' leads to 5'-exodeoxynuclease activity using a template that is transcribed faithfully rather than one that can undergo a slippage reaction.
mesh_terms: D001354:Avian Leukosis Virus / Q000201:enzymology*; D009189:Avian Myeloblastosis Virus / Q000201:enzymology* / Q000235:genetics; D004279:DNA, Viral / Q000096:biosynthesis*; D009838:Oligodeoxyribonucleotides / Q000378:metabolism; D011066:Poly C / Q000378:metabolism; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D004279:DNA, Viral; D009838:Oligodeoxyribonucleotides; D011066:Poly C; D012194:RNA-Directed DNA Polymerase