[Electron microscopic study of Bacillus subtilis mutants differing in the serine proteinase activity and spectrum].
Vegetative cells and spores of the colonial morphological mutants of Bacillus subtilis A-50 were studied by electron microscopy. The ultrastructure of vegetative cells from both asporogenic colonial-morphological mutants and those which were capable of forming spores in the presence of high concentrations of nitrogen and carbon sources with a decreased activity and a modified spectrum of serine proteases differed from the parent strain by the presence of a microcapsule, the uneven thickness of a cell wall, and the absence of a distinct periplasmic space. Crystalline inclusions of a regular shape were detected in the sporeforming mutant in those cells which were devoid of spores. Spores of the mutant had additional layers.
D001412:Bacillus subtilis / Q000201:enzymology / Q000648:ultrastructure*; D004789:Enzyme Activation; D008854:Microscopy, Electron; D009154:Mutation; D010447:Peptide Hydrolases / Q000378:metabolism*; D012694:Serine / Q000378:metabolism*; D013171:Spores, Bacterial / Q000648:ultrastructure*
D012694:Serine; D010447:Peptide Hydrolases
Elektronno-mikroskopicheskoe issledovanie mutantov Bacillus subtilis, otlichaiushchikhsia po aktivnosti i spektru serinovykh proteinaz.