title: Methylene blue and other agents as inhibitors of calcium oxalithiasis in vivo.
abstract: Growth of calcium oxalate on an established calculus upon a zinc nucleus in the bladder of rats was studied. Animals were fed either a regular solid chow or chow containing a potential crystal inhibitor ad libitum, along with drinking water containing 0.75 per cent ethylene glycol. Chow containing 0.2 per cent methylene blue and 0.5 per cent vitamin C not only decreased the growth rate, but calculi were much softer than those in controls. Safranin O was the only other significant growth inhibitor identified. Ethylenediamonotetraacetic acid and ethylenebis (oxyethylenenitrilo)-tetraacetic acid transformed the additional growth from the mono- to the dihydrate form of calcium oxalate.
mesh_terms: D000166:Acridines / Q000627:therapeutic use*; D000167:Acriflavine / Q000008:administration & dosage / Q000627:therapeutic use*; D000818:Animals; D001205:Ascorbic Acid / Q000008:administration & dosage / Q000627:therapeutic use*; D001399:Azure Stains / Q000008:administration & dosage / Q000627:therapeutic use*; D002129:Calcium Oxalate; D004492:Edetic Acid / Q000008:administration & dosage / Q000627:therapeutic use*; D004533:Egtazic Acid / Q000008:administration & dosage / Q000627:therapeutic use; D005026:Ethylene Glycols / Q000008:administration & dosage / Q000627:therapeutic use*; D008297:Male; D008751:Methylene Blue / Q000008:administration & dosage / Q000627:therapeutic use*; D010640:Phenothiazines / Q000627:therapeutic use*; D051381:Rats; D001744:Urinary Bladder Calculi / Q000139:chemically induced / Q000517:prevention & control*
chemical_list: D000166:Acridines; D001399:Azure Stains; D005026:Ethylene Glycols; D010640:Phenothiazines; D000167:Acriflavine; D002129:Calcium Oxalate; D004533:Egtazic Acid; D004492:Edetic Acid; D001205:Ascorbic Acid; D008751:Methylene Blue