Transfer RNA in aging Drosophila: II. Isoacceptor patterns.
Transfer RNA and aminoacyl-tRNA synthetases have been isolated from 5 and 35 day old Drosophila melanogaster males. The isoacceptor pattern of the following tRNAs is not changed irrespective of the source of the tRNA and amino-acylating enzymes: tRNAAla, tRNALeu, tRNAMet, and tRNASer. However, the Q base containing tRNAAsp, tRNAAsN, tRNAHis, and tRNATyr show dramatic changes in their isoacceptor patterns during the aging process. This probably reflects a change in the activity of a tRNA modifying enzyme. These findings are discussed in relation to Strehler's theory of aging and development.
D000375:Aging; D000596:Amino Acids / Q000378:metabolism; D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism*; D000818:Animals; D001216:Asparagine / Q000378:metabolism; D004331:Drosophila melanogaster; D006639:Histidine / Q000378:metabolism; D007930:Leucine / Q000378:metabolism; D008297:Male; D012343:RNA, Transfer / Q000378:metabolism*; D014443:Tyrosine / Q000378:metabolism
D000596:Amino Acids; D014443:Tyrosine; D006639:Histidine; D001216:Asparagine; D012343:RNA, Transfer; D000604:Amino Acyl-tRNA Synthetases; D007930:Leucine