Ultrastructure of an excitatory synapse.
The ultrastructure of the afferent synapse in hair cells of the lateral line-canal organ was studied using different fixation and staining techniques. Glutaraldehyde-fixed tissue without post-osmication, contrasted by section staining with uranyl acetate and lead citrate, was compared with (a) osmium tetroxide-fixed tissue followed by the same staining procedure, and with (b) glutaraldehyde-fixed tissue, block-impregnated with phosphotungstic acid (PTA). The results reveal a pronounced heterogeneity in the composition of the synaptic body, reflecting regional differences it the "intracleft substance", the synaptic structure defined by the PTA staining technique, is actually due to the glutaraldehyde fixation procedure and is apparently the outer leaflet of the postsynaptic membrane. A special technique that allows alternate sections of a series to be differentially stained for electron microscopy is proposed.
D000818:Animals; D005404:Fixatives; D005976:Glutaral; D006198:Hair Cells, Auditory / Q000648:ultrastructure*; D009993:Osmium Tetroxide; D010772:Phosphotungstic Acid; D013569:Synapses / Q000648:ultrastructure*; D013572:Synaptic Vesicles / Q000648:ultrastructure; D014501:Uranium
D005404:Fixatives; D010772:Phosphotungstic Acid; D014501:Uranium; D009993:Osmium Tetroxide; D005976:Glutaral