[Rule of antibody structure: the primary structure of a human monoclonal IgA1-immunoglobulin (myeloma protein Tro), III. Isolation and characterization of the tryptic peptides of the H-chain (author's transl)].
The reduced and either aminothylated or carboxymethylated H-chain of the monoclonal IgA1 immunoglobulin Tro was digested with trypsin. The tryptic peptides were isolated by gel and ion-exchange chromatography. Because of different methods of alkylation, the cysteine-containing peptides could be obtained in two forms and showed additional overlaps. Sequence studies performed with these fragments elucidated the primary structure of the protein.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D006801:Humans; D007070:Immunoglobulin A; D007143:Immunoglobulin Heavy Chains; D009101:Multiple Myeloma / Q000276:immunology; D009194:Myeloma Proteins; D010446:Peptide Fragments / Q000032:analysis; D014357:Trypsin
D000596:Amino Acids; D007070:Immunoglobulin A; D007143:Immunoglobulin Heavy Chains; D009194:Myeloma Proteins; D010446:Peptide Fragments; D014357:Trypsin
Zur Strukturregel der Antikörper. Die Primärstruktur eines monoklonalen IgA1-Immunglobulins (Myelomprotein Tro), III. Isolierung und Charakterisierung der tryptischen Peptide der H-Kette.