title: Isolation of stages of the human parasite Plasmodium falciparum from culture and from animal blood.
abstract: Procedures for isolation of various forms of the asexual erythrocytic stages of the human parasite Plasmodium falciparum are outlined. The procedures employ the plasma expander Physiogel, which is composed of a chemically modified, partially hydrolysed gelatin dissolved in Ringer's lactate. Based on the observation that parasitized cells which are easily separable by this technique differ appreciably at the ultrastructural level, a mechanism by which separation occurs is proposed.
mesh_terms: D000818:Animals; D001029:Aotus trivirgatus / Q000469:parasitology*; D000882:Haplorhini / Q000469:parasitology*; D006801:Humans; D010963:Plasmodium falciparum / Q000254:growth & development / Q000302:isolation & purification*; D012100:Reproduction, Asexual