title: Brucella abortus lipopolysaccharide is mitogenic for spleen cells of endotoxin-resistant C3H/HeJ mice.
abstract: Preparations of lipopolysaccharide (LPS) from rough and smooth strains of Brucella abortus were mitogenic for spleen cells of athymic nude mice, C3H/HeAU mice, and the endotoxin-resistant C3H/Hej mice. The mitogenic response induced by crude smooth-LPS (f5) was greater than that produced by purified smooth-LPS (f5p); however, the dose-response curves were similar for both preparations. The mitogenic activity of mouse spleen cells to both f5 and f5p was higher than that produced by stimulation with purified rough-LPS. The dose-response curves with rough-LPS were also qualitatively different from those produced with the preparations of smooth-LPS.
mesh_terms: D000818:Animals; D002003:Brucella abortus / Q000276:immunology*; D002417:Cattle; D004306:Dose-Response Relationship, Immunologic; D004731:Endotoxins / Q000494:pharmacology*; D004926:Escherichia coli / Q000276:immunology; D005260:Female; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008819:Mice, Nude; D008934:Mitogens / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000276:immunology; D013154:Spleen / Q000276:immunology*
chemical_list: D004731:Endotoxins; D008070:Lipopolysaccharides; D008934:Mitogens; D012710:Serum Albumin, Bovine