[Ultrastructural changes in the cells of a nonsynchronous Pseudomonas aeruginosa culture occurring under the influence of chlorhexidine bigluconate].
Total preparations of P. aeruginosa (strain 65) were studied after contrastive treatment with 2% phosphato-tungstic acid and 2% uranyl acetate; ultrathin sections of bacteria treated with chlorhexidine bigluconate in various concentrations and fixied by the method of Hoffmann et al. were also studied with the use of an electron microscope IEM 100 v. Structural and morphological changes depending on the concentration and the time of action of the antiseptic were discovered; these changes were manifested by bacterial lysis and coagulation, the lossening of the cell wall with revealing its five-layer structure.
D002462:Cell Membrane / Q000187:drug effects / Q000648:ultrastructure; D002710:Chlorhexidine / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005942:Gluconates / Q000494:pharmacology; D008854:Microscopy, Electron; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000648:ultrastructure; D013997:Time Factors
D005942:Gluconates; D002710:Chlorhexidine
Ul'trastrukturnye izmeneniia kletok nesinkhronnoĭ kul'tury Pseudomonas aeruginosa, voznikaiushchie pod vliianiem khlorgeksidina bigliukonata.