Genetic control of the immune response to the terpolymer L-glutamic acid 60-L-alanine 30-L-tyrosine10 (GAT). III. Restricted heterogeneity of the anti-GAT response from BALB/c responder mice.
The heterogeneity of the anti-GAT [terpolymer poly(Glu60, Ala30, Tyr10)] response of GAT responder mice has been analyzed. Purified anti-GAT antibodies from BALB/c mice belong only to the gamma 1 kappa subclass. The isoelectric focusing pattern obtained indicates that the anti-GAT antibodies are particularly basic and restricted. These results have been confirmed by two-dimensional polyacrylamide gel electrophoresis; by this technique, we have shown that the gamma 1 chain and the corresponding kappa chain of anti-GAT antibodies are restricted. All the anti-GAT antibodies from 10 BALB/c mice bear the cross-reactive GAT idiotype previously defined (J. Thèze and G. Sommé, Eur. J. Immunol. 1979, 9: 924). The present results indicate that the anti-GAT repertoire expressed in BALB/c mice is very limited.
D000818:Animals; D001202:Ascitic Fluid / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005802:Genes, MHC Class II; D007130:Immunoglobulin Idiotypes; D007525:Isoelectric Focusing; D051379:Mice; D008807:Mice, Inbred BALB C; D010455:Peptides / Q000276:immunology*
D007130:Immunoglobulin Idiotypes; D010455:Peptides