[Stability of Streptomyces griseus carboxypeptidase].
Carboxypeptidase of Str. griseus strain 32-13 is stable during storage at a temperature of 10-15 degrees C. Carbohydrates and glycerol in a concentration of 40-80% as well as 0.01 M of L-amino acid prevent the enzyme from thermoinactivation. Anions, such as phosphate, borate, carbonate and acetate, have their inhibitory effect on carboxypeptidase. After the effect of chelate reagents the enzyme activity restores completely only in the presence of Co2+ and partially in the presence of Ni2+ greater than Ca2+ greater than Mn2+ greater than Zn2+.
D000596:Amino Acids; D000838:Anions; D002268:Carboxypeptidases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002413:Cations, Divalent; D003035:Cobalt; D003080:Cold Temperature; D004355:Drug Stability; D004492:Edetic Acid; D005990:Glycerol; D010618:Phenanthrolines; D013305:Streptomyces griseus / Q000201:enzymology*
D000596:Amino Acids; D000838:Anions; D002413:Cations, Divalent; D010618:Phenanthrolines; D003035:Cobalt; D004492:Edetic Acid; D002268:Carboxypeptidases; D005990:Glycerol
O stabil'nosti karboksipeptidazy Streptomyces griseus.