title: Isolation of mutants deregulated in phosphate control of candicidin biosynthesis.
abstract: Mutants have been isolated in which phosphate does not inhibit the biosynthesis of candicidin. At high phosphate concentrations, candicidin production by phosphate-deregulated mutants is still inhibited, but to a lesser extent than in the wild type. Some of these mutants are higher candicidin producers than the wild type, not only in phosphate-supplemented medium but also in non-supplemented production medium. The high candicidin production by these mutants is due to (1) a high specific rate of candicidin biosynthesis and (2) an extended production phase. None of the phosphate-deregulated mutants in which uptake of [32P]phosphate was measured was a phosphate-permeability mutant.
mesh_terms: D000935:Antifungal Agents / Q000096:biosynthesis*; D002174:Candicidin / Q000096:biosynthesis*; D007700:Kinetics; D009154:Mutation; D010710:Phosphates / Q000378:metabolism / Q000494:pharmacology*; D013305:Streptomyces griseus / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism
chemical_list: D000935:Antifungal Agents; D010710:Phosphates; D002174:Candicidin