Isolation and characterization of an active variable domain from a homogeneous rabbit antibody light chain.
The variable domain (VL) of allotype b4 light chains of rabbit IgG was isolated from both nonimmune heterogeneous IgG and a homogeneous antibody directed against type III pneumococcal polysaccharide. Light chains were first isolated and then cleaved under mild acidic conditions between residues 109 and 110. Reduction with dithiothreitol in guanidine hydrochloride cleaved both intradomain disulfide bridges as well as the interdomain disulfide bridge joining the variable and constant domain. The sulfhydryl groups were protected after reduction by p-chloromercuribenzoate. VL was isolated from this mixture of variable and constant domains by affinity chromatography, utilizing sheep antibodies directed against a peptide including residues 110--211 from nonimmune IgG light chain. The isolated VL domain was identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and automated Edman degradation. VL from a homogeneous antibody was treated with dithiothreitol to remove p-chloromercuribenzoate, reoxidized, and recombined with homologous heavy chain. The binding of this recombinant to type III pneumococcal polysaccharide was identical with that of the light-chain--heavy-chain recombinant.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001666:Binding Sites, Antibody / Q000302:isolation & purification; D005779:Immunodiffusion; D007074:Immunoglobulin G / Q000302:isolation & purification; D007135:Immunoglobulin Variable Region / Q000302:isolation & purification; D008070:Lipopolysaccharides; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay; D012756:Sheep / Q000276:immunology
D000596:Amino Acids; D007074:Immunoglobulin G; D007135:Immunoglobulin Variable Region; D008070:Lipopolysaccharides