Oxidative and demethylating activity of multiple forms of laccase from Pholiota mutabilis.
1. The inducible and constitutive forms of laccase from the fungus Pholiota mutabilis show both the oxidative and demethylating activity, which proves the bifunctional character of the enzyme. 2. The oxidative/demethylating activity ratio of the forms induced either with ferulic or syringic acid is different from that shown by the constitutive form. 3. Splitting of one methoxyl group from the methoxyphenol substrate is associated with the release of one molecule of methanol.
D000363:Agaricales / Q000201:enzymology*; D004156:Catechol Oxidase / Q000302:isolation & purification / Q000378:metabolism*; D003373:Coumaric Acids / Q000494:pharmacology; D004790:Enzyme Induction; D005707:Gallic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D007202:Indicators and Reagents / Q000494:pharmacology; D010084:Oxidation-Reduction
D003373:Coumaric Acids; D007202:Indicators and Reagents; D005707:Gallic Acid; C004999:ferulic acid; C001945:syringic acid; D004156:Catechol Oxidase