Separation of oligonucleotides on ion-exchange derivatives of Spheron.
Diphenylamine-formic acid hydrolyzate of Bacillus subtilis DNA (a mixture of pyrimidine oligodeoxyribonucleotides) was chromatographed using an ionic strength gradient on seven ion exchangers: five DEAE-Spherons 300 of various capacities, two types of BD-Spheron 300 and DEAE-Sephadex A-25. The suitability of ion-exchange derivatives of Spheron for the chromatography of oligonucleotides is discussed. The separation of mono- to undeca-deoxyribonucleotides was achieved.
D001412:Bacillus subtilis / Q000032:analysis*; D002852:Chromatography, Ion Exchange / Q000379:methods; D004269:DNA, Bacterial; D009841:Oligonucleotides / Q000302:isolation & purification*
D004269:DNA, Bacterial; D009841:Oligonucleotides