Interaction of the mannosephilic lectins of Pseudomonas aeruginosa with luminous species of marine enterobacteria.
The marine bacteria Beneckea harveyi and Photobacterium leiognathi were shown to bear mannose-containing binding sites for the mannosephilic lectins of Pseudomonas aeruginosa and concanavalin A (Con A). The interaction between the lectins and the marine bacteria was demonstrated by the bacteriagglutination test, by adsorption of the lectins onto the bacteria and by mannose-specific peroxidase-binding to the lectin-coated bacteria. Treatment of the bacteria with formaldehyde, phenol, ethanol or boiling them for 15 min, did not alter their ability to adsorb the lectins. The growth rate of the marine bacteria was unaffected when either the Pseudomonas lectins or Con A was added to the culture medium.
D003208:Concanavalin A / Q000494:pharmacology; D004754:Enterobacter / Q000145:classification* / Q000187:drug effects / Q000302:isolation & purification; D004755:Enterobacteriaceae / Q000145:classification*; D006384:Hemagglutination; D037102:Lectins / Q000378:metabolism / Q000494:pharmacology*; D008163:Luminescent Measurements; D008358:Mannose / Q000378:metabolism; D010776:Photobacterium / Q000145:classification / Q000187:drug effects / Q000302:isolation & purification; D011550:Pseudomonas aeruginosa / Q000378:metabolism; D014733:Vibrio / Q000145:classification / Q000187:drug effects / Q000302:isolation & purification
D037102:Lectins; D003208:Concanavalin A; D008358:Mannose