Experimentally induced autoantibody to heat-stable alkaline phosphatase in the baboon.
Experimental evidence has been collected which signifies that autoantibody has been induced against lung heat-stable alkaline phosphatase which represented 60% of the total alkaline phosphatase of that tissue. Immunization of a male baboon with highly purified human placental alkaline phosphatase (heat-stable and cross-reactive with the baboon heat-stable enzyme) resulted in production of a precipitating factor in the immune serum which reacted with the heat-stable enzyme of both the human and baboon but not the heat-labile form of alkaline phosphatase of either species. This precipitating factor is a baboon autoantibody because 1) it had a gamma mobility on immunoelectrophoresis and retarded the electrophoretic mobility of the heat-stable enzyme from both normal and immunized baboons; 2) its titer increased as more booster injections were administered; 3) it formed a well-defined precipitin rocket with the baboon heat-stable enzyme in the Lurell's antigen-antibody crossed electrophoresis; 4) in immunodiffusion it formed a discrete precipitin line with the baboon heat-stable enzyme, which fused partially with the precipitin line of human placental alkaline phosphatase (immunogen).
D000469:Alkaline Phosphatase / Q000276:immunology*; D000818:Animals; D001323:Autoantibodies; D004588:Electrophoresis, Cellulose Acetate; D000882:Haplorhini; D006358:Hot Temperature; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D005779:Immunodiffusion; D007123:Immunoelectrophoresis, Two-Dimensional; D007422:Intestines / Q000201:enzymology; D007668:Kidney / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008168:Lung / Q000201:enzymology; D008297:Male; D009206:Myocardium / Q000201:enzymology; D010215:Papio; D010920:Placenta / Q000201:enzymology; D011817:Rabbits
D001323:Autoantibodies; D007106:Immune Sera; D000469:Alkaline Phosphatase