title: Sugar hydrolases of the infant rat intestine and their arrangement of the brush border membrane.
abstract: Lactase and maltase, the predominant sugar hydrolases associated with the intestinal brush bordermembrane of the suckling rat, were purified essentially free of the other to near homogeneity (lactase at specific activity 23, maltase at specific activity 58), and their specific physiocochemical properties determined. Antisera prepared to each showed by immunodiffusion a single common precipitin line with pure enzyme and solubilized proteins of the brush border membrane. Brush border membranes were purified 26--35-fold from infant rat intestine. Membranes prepared from 10-day-old rats contained 32% protein, 43% lipid and 25% carbohydrate with lactase and maltase estimated to comprise in excess of 10% and 2%, respectively, of the membrane protein. Immunotitration curves of lactase and maltase showed equivalent antibody binding by the membrane-bound and free enzyme forms. Furthermore, antibody binding to one enzyme did not affect the immunotitration curve or the extractability (by papain or Triton X-100) of the other membrane-bound enzyme. It was concluded that the lactase and maltase molecules are attached singly on the external membrane surface in a spatially independent manner with their antigenic sites as freely available to antibody binding as exhibited by their papain-solubilized counterparts.
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002462:Cell Membrane / Q000201:enzymology*; D005696:Galactosidases / Q000032:analysis*; D005959:Glucosidases / Q000032:analysis*; D005779:Immunodiffusion; D007413:Intestinal Mucosa / Q000201:enzymology* / Q000648:ultrastructure; D008871:Microvilli / Q000201:enzymology* / Q000648:ultrastructure; D051381:Rats; D000520:alpha-Glucosidases / Q000032:analysis* / Q000302:isolation & purification; D001616:beta-Galactosidase / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D005696:Galactosidases; D005959:Glucosidases; D000520:alpha-Glucosidases; D001616:beta-Galactosidase