[Different primary structure of 2 variants of Friend virus p 30 polypeptide separated by isoelectric focusing].
Variants of p 30 (iso-p 30s), the 30,000 dalton major polypeptide of murine C-type retraviruses, have been characterized in all virus strains by isoelectric focusing. Several of these iso-p 30s have been found to coexist in a given virus strain. In the present study, two iso-p 30s, characteristic of the Friend-Rauscher subgroup, separated by preparative isoelectric focusing of p 30 in thin layers of polydextran gel, were subjected to tryptic peptide mapping and aminoacid analysis. The two iso-p 30s, with isoelectric points (pI) of 6.5 and 7.1, were found to possess a partially different primary structure, with about 50% homology. The results are discussed.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D002850:Chromatography, Gel; D005622:Friend murine leukemia virus / Q000032:analysis*; D007525:Isoelectric Focusing / Q000379:methods; D010455:Peptides / Q000032:analysis* / Q000302:isolation & purification; D051381:Rats; D014764:Viral Proteins / Q000032:analysis* / Q000302:isolation & purification
D000596:Amino Acids; D010455:Peptides; D014764:Viral Proteins
Structure primaire différente de deux variants du polypeptide p 30 du virus de Friend séparés par focalisation au point iso-électrique.