title: The relative thickness of intracellular membranes in epithelial cells of the ventral lobe of the rat prostate.
abstract: The relative thickness of intracellular membranes of epithelial cells in the ventral lobe of the rat prostrate was measured by a densitometric method. Glutaraldehyde perfusion followed by ruthenium tetroxide immersion fixation appeared to be the most suitable method for membrane thickness measurements. By thickness, the membranes could be roughly subdivided into three groups. The inner and outer membranes of the mitochondrion made up the thinnest membranes of the cell. The second group of membranes consisted of the membranes of the rough-surfaced endoplasmic reticulum and the Golgi apparatus, the different faces of the latter organelle, and the Golgi vesicles. The thickest group of membranes included those of the cell membrane, secretory granules, condensing vacuoles, lysosomes, autophagic vacuoles and multivesicular bodies. The differences in thickness of the membranes are probably due to the varying protein/lipid ratio, and the qualities and proportions of the different lipids in the membranes.
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000648:ultrastructure; D003720:Densitometry; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D004848:Epithelium / Q000648:ultrastructure; D005976:Glutaral; D006056:Golgi Apparatus / Q000648:ultrastructure; D007425:Intracellular Membranes / Q000648:ultrastructure*; D008247:Lysosomes / Q000648:ultrastructure; D008297:Male; D011467:Prostate / Q000648:ultrastructure*; D051381:Rats; D012428:Ruthenium; D014617:Vacuoles / Q000648:ultrastructure
chemical_list: D012428:Ruthenium; D005976:Glutaral