Characterization of stem cells and progenitors of hemopoiesis by cell sorting.
Cell sorting has been used as a method for characterizing hemopoietic stem cells and progenitors. Fluorescent antibody-surface labels and changes in fluorescence polarization induced by in vitro stimulation with potential hemopoietic regulators were used. As detected by significant enrichment of CFU-S (pluripotent stem cells) in fluorescence-activated cell sorting, some CFU-S bear 'unique antigens' recognized by rabbit anti-human brain sera, human anti-human sperm sera, and 129 anti-F9 serum, but not A . TH anti-A . TL (Ia) ascites. Significant changes in fluorescence polarization induced by in vitro stimulation of mouse bone marrow with potential hemopoietic regulators were also observed; further, progenitors of human T-lymphocyte colonies were observed to exhibit a significantly decreased mean polarization value after short-term stimulation with PHA-LCM (phytohemagglutinin-stimulated leukocyte conditioned medium).
D000818:Animals; D000954:Antigens, Surface; D001853:Bone Marrow / Q000276:immunology; D002469:Cell Separation / Q000379:methods; D002999:Clone Cells / Q000276:immunology; D003114:Colony-Forming Units Assay; D000939:Epitopes; D005260:Female; D005455:Fluorescent Antibody Technique; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D006801:Humans; D008297:Male; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology
D000954:Antigens, Surface; D000939:Epitopes