title: TOL is a broad-host-range plasmid.
abstract: We readily isolated insertions of the carbenicillin resistance element Tn401 into the TOL plasmid in Pseudomonas putida. Hybrid TOL::Tn401 plasmids stably express the Cbr phenotype in Pseudomonas aeruginosa and Escherichia coli. Whereas the replicative and conjugative functions are expressed in both hosts, the ability to grow on m-toluate is only expressed in the Pseudomonas species.
mesh_terms: D003227:Conjugation, Genetic; D004926:Escherichia coli / Q000235:genetics; D010403:Penicillin Resistance; D010957:Plasmids; D011549:Pseudomonas / Q000235:genetics* / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000235:genetics; D011815:R Factors; D011995:Recombination, Genetic; D014050:Toluene / Q000378:metabolism*; D014992:Xylenes / Q000378:metabolism*
chemical_list: D014992:Xylenes; D014050:Toluene