[Comparative sensitivity of 4 different cell cultures in the isolation and identification of mumps virus].
Three different continuous cell lines (Am 57, Hela, Vero) and primary cell culture of human embryo kidney are compared with regard to their susceptibility for isolation of mumps virus from saliva and cerebrospinal fluid. For this purpose the Vero cell line appeared to be more suitable among the studied cell systems regarding the following reasons: it allows the highest percentage of mumps virus isolation. The cytopathic effect caused by mumps virus occurs comparatively rapidly in this cell culture. This cytopathic effect is typical enough to allow for a preliminary diagnosis of mumps virus.
D000818:Animals; D002460:Cell Line; D002555:Cerebrospinal Fluid / Q000382:microbiology; D004622:Embryo, Mammalian; D000882:Haplorhini; D006367:HeLa Cells; D007668:Kidney; D009109:Mumps virus / Q000302:isolation & purification*; D012463:Saliva / Q000382:microbiology; D013997:Time Factors
Sensibilité comparée de quatre cultures cellulaires différentes pour l'isolement et l'identification de virus des oreillons.