title: [Experiments to establish the most suitable method for the production and control of conjugated sera].
abstract: Studies aiming to establish the most suitable method for conjugated serum production and control were carried out. Results obtained indicate that purest globulin fractions are obtained in case the serum is precipitated by 50% saturation with (NH4)2.SO4, then the sediment is washed with 50% (NH4)2SO4, dissolved in water and precipitated again by 50% saturation with (NH4)2SO4. Conjugated titer is highest when conjugation is performed at a 4:100 FITC (fluorescin-iso-thio-cyanate):protein ratio. The produced conjugated serums are specific and are release with a working titer not lower that 1:4.
mesh_terms: D000818:Animals; D002003:Brucella abortus / Q000276:immunology; D002168:Campylobacter fetus / Q000276:immunology; D011232:Chemical Precipitation; D007106:Immune Sera / Q000302:isolation & purification*; D007114:Immunization; D007158:Immunologic Techniques; D010527:Perissodactyla / Q000276:immunology; D011817:Rabbits; D012712:Serum Globulins / Q000302:isolation & purification
chemical_list: D007106:Immune Sera; D012712:Serum Globulins
vernacular_title: Opiti za ustanoviavane na naĭ-podkhodiasht metod za proizvodstvo i kontrol na koniugirani serumi.