Antigenic analysis of sequential erythrocytic stages of Plasmodium knowlesi.
The antigenic composition of sequential erythrocytic stages of Plasmodium knowlesi has been compared by crossed immuno-electrophoresis using a pool of immune rhesus monkey antiserum. Eleven major parasite antigens have been identified; 9 are stage-independent, and 2 stage-dependent. Differences in the relative amount of the stage-independent antigens have been demonstrated and quantified. The distribution of antigens between parasites and schizont and infected red cell membranes has been examined. Only 6 of the 11 parasite antigens were exhibited by a schizont membrane preparation, all these antigens were also expressed by the intracellular parasite. Antigens exclusive to the schizont membrane were not demonstrated.
D000818:Animals; D000941:Antigens / Q000032:analysis* / Q000302:isolation & purification; D004912:Erythrocytes / Q000276:immunology / Q000469:parasitology*; D005260:Female; D000882:Haplorhini; D007123:Immunoelectrophoresis, Two-Dimensional; D008253:Macaca mulatta; D008288:Malaria / Q000469:parasitology; D008297:Male; D010961:Plasmodium / Q000254:growth & development / Q000276:immunology* / Q000302:isolation & purification
D000941:Antigens