Polymer-linked 6-azauridine 5'-monophosphate, a resin of high bioaffinity to orotidine-5'-phosphate decarboxylase.
Condensation of 6-azauridine with ethyl levulinate, followed by saponification or phosphorylation, leads to 2',3'-O-[1-(2-carboxyethyl)ethylidene]-6-azauridine and its 5'-monophosphate. The latter was coupled to 6-aminohexylagarose via its carboxylic group. Using the same synthetic route, agarose-linked uridine 5'-monophosphate has been prepared. Both polymers show specific binding toward orotidine-5'-monophosphate decarboxylase. The immobilized inhibitor (6-azauridine 5'-monophosphat) binds the enzyme more strongly than the immobilized uridine 5'-monophosphate. Both resins have been used to separate orotidine-5'-monophosphate decarboxylase from orotidine-5'-monophosphate pyrophosphorylase.
D001380:Azauridine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000494:pharmacology; D002262:Carboxy-Lyases / Q000037:antagonists & inhibitors*; D002846:Chromatography, Affinity; D009964:Orotidine-5'-Phosphate Decarboxylase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D012117:Resins, Synthetic / Q000138:chemical synthesis* / Q000494:pharmacology; D014500:Uracil Nucleotides / Q000138:chemical synthesis*; D014542:Uridine Monophosphate / Q000031:analogs & derivatives / Q000138:chemical synthesis* / Q000494:pharmacology
D012117:Resins, Synthetic; D014500:Uracil Nucleotides; D001380:Azauridine; D014542:Uridine Monophosphate; D002262:Carboxy-Lyases; D009964:Orotidine-5'-Phosphate Decarboxylase