title: Cell-mediated and humoral immune responses of cattle to Brucella abortus, Mycobacterium bovis, and tetanus toxoid: evaluation of immunization and assay techniques.
abstract: A concentration of 2.5 X 10(-5) M 2-mercaptoethanol (2-ME) added to the medium in lymphocyte blastogenesis assays increased both the uptake of [3H]thymidine in unstimulated lymphocyte cultures and the probability of detecting antigen-sensitized cattle. The use of 2-ME did not cause lymphocytes from unsensitized cattle to react positively in blastogenesis assays. A crude brucella lysate prepared from Brucella abortus strain 19 was compared with a well-characterized brucella protein allergen prepared from B melitensis and was found to be equally suitable for use in blastogenesis assays. Cell-mediated immunity was produced most effectively in 4-month-old calves by tetanus toxoid, then by Mycobacterium bovis, and least effectively by B abortus.
mesh_terms: D000818:Animals; D000917:Antibody Formation; D002003:Brucella abortus / Q000276:immunology*; D002417:Cattle / Q000276:immunology*; D007111:Immunity, Cellular; D007114:Immunization / Q000662:veterinary*; D008213:Lymphocyte Activation; D008623:Mercaptoethanol / Q000494:pharmacology; D009163:Mycobacterium bovis / Q000276:immunology*; D012882:Skin Tests; D013745:Tetanus Toxoid / Q000276:immunology*
chemical_list: D013745:Tetanus Toxoid; D008623:Mercaptoethanol