title: In vitro inhibition of digestive enzymes by heavy metals and their reversal by chelating agent: Part II. Lead nitrate intoxication.
abstract: The effect of lead on alkaline phosphatase, lipase, aminotripeptidase and glycylglycine dipeptidase in the liver and digestive tract of Channa punctatus is investigated in vitro. Mercury inhibits the activities of all these enzymes and the degree of inhibition increased with the increase in the concentration of the metal. Addition of EDTA, a chelating agent, restored the mercury inhibited enzyme activity and the degree of restoration was related to the concentration of the chelating agent.
mesh_terms: D000818:Animals; D002432:Cecum / Q000201:enzymology; D004064:Digestive System / Q000201:enzymology*; D004492:Edetic Acid / Q000494:pharmacology*; D004791:Enzyme Inhibitors; D005399:Fishes / Q000378:metabolism*; D066298:In Vitro Techniques; D007422:Intestines / Q000201:enzymology; D007854:Lead / Q000037:antagonists & inhibitors; D007855:Lead Poisoning / Q000201:enzymology*; D008099:Liver / Q000201:enzymology; D013270:Stomach / Q000201:enzymology
chemical_list: D004791:Enzyme Inhibitors; D007854:Lead; D004492:Edetic Acid