Kinetic behaviour under defined assay conditions for bovine lens aldose reductase.
Bovine lens aldose reductase, an enzyme important in sugar cataract formation, has been purified using a new procedure, and its optimum conditions of assay defined in both lens homogenates and purified preparations. Preliminary kinetic analysis shows that the degree of the rate equation is of a low order, and may be Michaelian under the standard conditions used.
D000449:Aldehyde Reductase / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D007700:Kinetics; D007908:Lens, Crystalline / Q000201:enzymology*; D013401:Sugar Alcohol Dehydrogenases / Q000378:metabolism*
D013401:Sugar Alcohol Dehydrogenases; D000449:Aldehyde Reductase