Influence of Ca2+ ions and cation chelators on activity measurement of creatine kinase isoenzymes.
EDTA and EGTA, added to the reaction mixture for the activity determination of creatine kinase, stimulate the activity of creatine kinase to various extents by suppressing the inhibitory effect of Ca2+ ions. The activation effect is highest for isoenzyme BB, less for isoenzyme MB, and lowest for isoenzyme MM.
D002118:Calcium / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D003402:Creatine Kinase / Q000378:metabolism*; D004492:Edetic Acid / Q000494:pharmacology*; D004533:Egtazic Acid / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D005026:Ethylene Glycols / Q000494:pharmacology*; D006801:Humans; D007527:Isoenzymes
D005026:Ethylene Glycols; D007527:Isoenzymes; D004533:Egtazic Acid; D004492:Edetic Acid; D003402:Creatine Kinase; D002118:Calcium