[Radioautographic identification of dopaminergic axons in the rat CNS. In vitro marking with tritiated dopamine or noradenaline].
A new radioautographic technique for the visualization of central dopaminergic fibers is described. This technique is based upon in vitro uptake of triatiated norepinephrine or tritiated dopamine in the presence of monoamine oxidase inhibitor and specific uptake inhibitor in noradrenergic fibers. After a short step in glutaraldehyde, the use of osmium vapours as a fixative, instead of osmic acid immersion, allows a satisfactory retention of triatiated amines within dopaminergic fibers.
D000818:Animals; D001345:Autoradiography; D001369:Axons / Q000032:analysis* / Q000187:drug effects / Q000378:metabolism; D001923:Brain Chemistry; D003891:Desipramine / Q000494:pharmacology; D004298:Dopamine / Q000032:analysis* / Q000378:metabolism; D009638:Norepinephrine / Q000378:metabolism; D051381:Rats; D014316:Tritium
D014316:Tritium; D003891:Desipramine; D004298:Dopamine; D009638:Norepinephrine
Identification radioautographique des axones dopaminergiques dans le SNC du rat. Marquage in vitro par la dopamine ou la noradrénaline tritiées.