Isolation of tubercle bacteria using Ogawa egg medium modified by addition of Tween 80.
A modified medium was developed by altering the composition of 3 per cent Ogawa egg medium and adding Tween 80. Isolation of tubercle bacilli using this medium, in general, showed better growth and colines became visible earlier than the original medium, but there was no improvement in the positive rate.
D003470:Culture Media; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000254:growth & development / Q000302:isolation & purification*; D011136:Polysorbates; D013183:Sputum / Q000382:microbiology; D014397:Tuberculosis, Pulmonary / Q000382:microbiology*
D003470:Culture Media; D011136:Polysorbates