In vitro aggregation of mouse peritoneal exudate cells.
(1) Immunological mechanisms which cause macrophage agglutination in vitro have been investigated. (2) Quantitative differences in the agglutinating properties of sero-positive rheumatoid sera and normal sera were found. (3) NZB X W mouse peritoneal macrophages differed from normal (NZCW) mouse peritoneal macrophages in susceptibility to agglutination. These quantitative differences could be explained by NZB X W macrophages carrying a surface immunoglobulin component which increased agglutination by rheumatoid sera. (4) Antimacrophage antibody was more effective in rendering macrophages susceptible to the agglutinating effect of antiglobulins than was heat-aggregated gamma globulin.
D000372:Agglutination Tests; D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology*; D002449:Cell Aggregation; D007106:Immune Sera; D007114:Immunization; D066298:In Vitro Techniques; D008264:Macrophages / Q000276:immunology*; D008297:Male; D051379:Mice; D008814:Mice, Inbred NZB; D012217:Rheumatoid Factor; D005719:gamma-Globulins
D007106:Immune Sera; D005719:gamma-Globulins; D012217:Rheumatoid Factor