Prenatal protein depletion and delta 9, delta 6 and delta 5 desaturases in the rat.
Pregnant rats were kept throughout gestation on a control diet (i.e., 25% protein), on a low protein diet (i.e., 5% protein) or on a fat-free diet. At 20--21 days of gestation, the rate of 9-, 6-, and 5-desaturation was measured, using microsomes from maternal and fetal livers and placenta microsomes. The effect of protein malnutrition was more evident upon delta 6-desaturase activity from maternal liver, while a less severe reduction in the activities of delta 9- and delta 5-desaturases was observed. No measurable activities of delta 5- and delta 6-desaturases were observed in fetal liver and placenta, while a low activity of delta 9-desaturase was detected in both tissues from the three groups under study. We concluded that delta 6-desaturation is greatly affected by maternal protein deprivation, and this fact could affect the normal supply of polyunsaturated fatty acids for the normal fetus growth and tissue development.
D000818:Animals; D004041:Dietary Fats; D004044:Dietary Proteins; D044943:Fatty Acid Desaturases / Q000378:metabolism*; D005260:Female; D005333:Fetus; D008861:Microsomes / Q000201:enzymology*; D008862:Microsomes, Liver / Q000201:enzymology*; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D011502:Protein-Energy Malnutrition / Q000201:enzymology*; D051381:Rats; D013379:Substrate Specificity
D004041:Dietary Fats; D004044:Dietary Proteins; D044943:Fatty Acid Desaturases