Maltotetraose as a substrate for enzyme-coupled assay of amylase activity in serum and urine.
The use of maltotetraose ss a new substrate for the enzyme-coupled determination of amylase activity in biological fluids was developed by Beckman Microbics. We evaluated a manual and a centrifugal analyzer version of the method in comparison with two commonly used manual starch-dye amylase techniques: Roche Amylochrome and Pharmacia Phadebas. Both maltotetraose amylase procedures proved to be rapid and precise, and results correlated satisfactorily with the starch-dye methods for serum and urine samples.
D000681:Amylases / Q000097:blood* / Q000652:urine; D000900:Anti-Bacterial Agents; D001322:Autoanalysis; D006801:Humans; D007700:Kinetics; D008320:Maltose / Q000031:analogs & derivatives; D009844:Oligosaccharides; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
D000900:Anti-Bacterial Agents; D009844:Oligosaccharides; D008320:Maltose; C009819:maltotetraose; D000681:Amylases