Analysis of hyaluronidase diffusion for histochemical purposes.
Observations on hyaluronidase difussion from natural and artificial objects to precipitated medium are described. The conclusions from this investigation are: (1) hyaluronidase diffusing from biological objects in a precipitated medium is an active enzyme. (2) hyaluronidase diffusion may be utilized for localization of the enzyme. (3) certain parameters of the reaction hyaluronidase--hyaluronic acid may be measured with the use of the notion "diffusion radius".
D000818:Animals; D004058:Diffusion; D006651:Histocytochemistry; D006821:Hyaluronoglucosaminidase / Q000032:analysis; D008297:Male; D051381:Rats; D012671:Seminiferous Tubules / Q000201:enzymology; D013737:Testis / Q000166:cytology / Q000201:enzymology
D006821:Hyaluronoglucosaminidase