[Effect of multivalent cations on the enzymatic determination of glucose with glucose oxidase].
Iron and copper ions, in a concentration greater than 10(-4) M, inhibit the indicator reaction of a glucose oxidase-peroxidase reagent for the enzymatic determination of glucose, when weakly complexing buffers or buffer-free reaction media are used. The addition of EDTA and other complexing agents or, time-dependently, the buffer ions themselves reverse the inhibition to a great extent. The discussed mechanism of inhibition is based on the assumption that the metal ions share in the re-oxidation of the co-enzyme of glucose oxidase.
D002413:Cations, Divalent; D004492:Edetic Acid / Q000494:pharmacology; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000378:metabolism; D007501:Iron / Q000494:pharmacology; D007700:Kinetics; D008722:Methods
D002413:Cations, Divalent; D004492:Edetic Acid; D007501:Iron; D005949:Glucose Oxidase; D005947:Glucose
Zum Einbluss mehrwertiger Kationen auf die enzymatische Bestimmung von Glucose mit Glucoseoxydase.