title: 5-(alpha-Bromoacetyl)-2'-deoxyuridine 5'-phosphate: an affinity label for thymidylate synthetase.
abstract: 5-(alpha-Bromoacetyl)-2'-deoxyuridine 5'-phosphate (1) is an active-site-directed irreversible inhibitor of thymidylate synthetase from Lactobacillus casei. Analysis of the rate of inactivation of the enzyme in the presence of substrate confirmed the intermediate formation of a reversible enzyme-inhibitor complex.
mesh_terms: D000345:Affinity Labels / Q000138:chemical synthesis*; D001665:Binding Sites; D003856:Deoxyuracil Nucleotides / Q000138:chemical synthesis* / Q000494:pharmacology; D007700:Kinetics; D007780:Lacticaseibacillus casei / Q000201:enzymology; D008780:Methyltransferases / Q000037:antagonists & inhibitors*; D008954:Models, Biological; D013940:Thymidylate Synthase / Q000037:antagonists & inhibitors*
chemical_list: D000345:Affinity Labels; D003856:Deoxyuracil Nucleotides; D008780:Methyltransferases; D013940:Thymidylate Synthase