An interpretation of the apparent dual specificity of some murine myeloma immunoglobulins with inulinbinding activity.
Five murine myeloma immunoglobulins have been studied for their binding with fructose-containing homopolysaccharides. The results indicate that the anti-(2 leads to 1)-D-fructofuranans are capable of binding (2 leads to 6)-D-fructofuranans with reduced affinity. An anti-(2 leads to 6)-D-fructofuranan (U10) was incapable of binding a (2 leads to 1)-D-fructofuranan. By using molecular models of fructofuranans of both linkage types, and a hypothetical molecular model of the combining region of E109, an anti-(2 leads to 1)-D-fructofuranan, a rationalization of these date can be proposed.
D000818:Animals; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D005455:Fluorescent Antibody Technique; D005630:Fructans / Q000276:immunology; D005779:Immunodiffusion; D007140:Immunoglobulin Fab Fragments / Q000276:immunology*; D007444:Inulin / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009194:Myeloma Proteins / Q000276:immunology*
D005630:Fructans; D007140:Immunoglobulin Fab Fragments; D009194:Myeloma Proteins; D007444:Inulin