title: Biochemical and immunological studies of three genetic variants of 3-phosphoglycerate kinase 2 from the mouse.
abstract: Three electrophoretic variants of 3-phosphoglycerate kinase 2 (PGK-2A,PGK-2B, and PGK-2C) were purified from DBA/2J, C3H/HeJ, and C57L/J mice, respectively. PGK-2C exhibits only 2% of the specific activity of PGK-2A and PGK-2B in the reaction leading to the formation of 1,3-diphosphoglycerate. Compared to PGK-2A and PGK-2B, PGK-2C exhibits broader coenzyme specificity and lower Kms for substrate and coenzymes.  Incubation at 45C revealed immunionactivation and double immunodiffusion studies showed that mice carrying any one of these three PGK-2 alleles have similar amounts of proteins for PGK-1 and PGK-2 in testes.  The results of these studies suggest that low PGK-2C activity in C57L/J mice is a result of a structural rather than a regulatory gene mutation.
mesh_terms: D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D000939:Epitopes; D005796:Genes; D014644:Genetic Variation; D007525:Isoelectric Focusing; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H / Q000235:genetics; D008810:Mice, Inbred C57BL / Q000235:genetics; D008811:Mice, Inbred DBA / Q000235:genetics; D010735:Phosphoglycerate Kinase / Q000235:genetics* / Q000276:immunology; D013737:Testis / Q000201:enzymology
chemical_list: D000939:Epitopes; D010735:Phosphoglycerate Kinase