Some structural properties of thymus leukemia antigen (TL) solubilized with detergent.
Plasma membranes were isolated from the leukemia cell line ASL1w and extracted with detergent (DOC). DOC solubilized more TL activity than could be detected on isolated membranes. However, extraction of membranes with LDS or EDTA solubilized only 17% and 4%, respectively, of the activity. This indicated that TL was not loosely associated with the membrane but rather was integrated into the lipid bilayer. At low concentrations of DOC (0.05%), TL was found to be largely aggregated and was also prone to autolysis. Neither aggregation nor autolysis was observed at a higher DOC concentration (0.5%). The apparent molecular weight of TL in 0.5% DOC was determined by Sephadex G-200 chromatography to be about 65,000-70,000. Digestion of a 0.5% DOC extract of TL with either papain or trypsin produced a fragment of TL of about 35,000 molecular weight. These fragments were similar in size to a fragment produced by autolysis. These data suggested that a region of the TL molecule was very prone to proteolytic attack. The 35,000 molecular weight proteolytic fragments bound specifically to lentil lectin affinity columns, which indicated that they retained at least part of the carbohydrate present on the native molecule.
D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis; D002460:Cell Line; D002850:Chromatography, Gel; D003601:Cytotoxicity Tests, Immunologic; D003902:Detergents / Q000494:pharmacology; D000939:Epitopes; D037102:Lectins / Q000276:immunology; D007942:Leukemia, Experimental / Q000276:immunology*; D051379:Mice; D008970:Molecular Weight; D010206:Papain / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology*; D013950:Thymus Gland / Q000276:immunology; D014357:Trypsin / Q000494:pharmacology
D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D003902:Detergents; D000939:Epitopes; D037102:Lectins; D014357:Trypsin; D010206:Papain