Analysis of anti-sperm sera for T/t locus-specific antibody.
An objective antiglobulin radioimmunoassay for the analysis of anti-spermatozoa antisera has been used to characterize more than 30 alloantisera. Each of these antisera was raised against spermatozoa from mice carrying T/t regin mutations. Whilst all of the antisera had considerable activity for mouse spermatozoa, none of them was found to contain antibodies specific for T/t region gene products. A detailed analysis of a single serum revealed that its activity is for antigens which are non-polymorphic, sperm-specific and species-specific. The failure in this study to detect antibodies specific for T/t region gene products is discussed.
D000042:Absorption; D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D000918:Antibody Specificity; D001665:Binding Sites; D002874:Chromosome Mapping; D000939:Epitopes; D007106:Immune Sera; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D009154:Mutation; D011817:Rabbits; D011863:Radioimmunoassay; D051381:Rats; D013094:Spermatozoa / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D000939:Epitopes; D007106:Immune Sera