[Effect of sources of carbon, nitrogen, and phosphorus on the synthesis of proteases from Bacillus subtilis cultures].
The paper gives data on the influence of different sources of carbon, nitrogen and phosphorus on the accumulation of biomass and synthesis of neutral protease by Bacillus subtilis str. 103. The highest proteolytic activity was obtained on the medium containing maltose or hydrolyzed starch as a carbon source, monopotassium phosphate as a phosphorus source, and ammonium sulphate as a nitrogen source. The enzyme activity increased upon an addition of albumin, peptone or casein. A study of the amino acid effect showed that methionine, isoleucine and valine stimulated the protease synthesis to the greatest extent.
D000596:Amino Acids / Q000494:pharmacology; D001412:Bacillus subtilis / Q000201:enzymology* / Q000254:growth & development; D002244:Carbon; D003470:Culture Media; D010450:Endopeptidases / Q000096:biosynthesis* / Q000378:metabolism; D006868:Hydrolysis; D009584:Nitrogen; D010758:Phosphorus
D000596:Amino Acids; D003470:Culture Media; D010758:Phosphorus; D002244:Carbon; D010450:Endopeptidases; D009584:Nitrogen
Vliianie istochnikov ugleroda, azota, fosfora na sintez proteazy kul'turoĭ Bacillus subtilis.