In vitro colony growth of granulocytes, macrophages, T and B lymphocytes in agar capillaries.
Compared with the common agar of hematopoietic colonies in Petri dishes, the use of glass capillary tubes offers a couple of advantages which have been exploited. Among these advantages are less materials, less cells to be seeded, lower costs, easier and quicker colony counting by visual and semi-automated optical scanning, and lower risk of bacterial contamination. In tubes several parameters affecting the colony formation of mouse and human granulocytes and macrophages as well as of human peripheral T and B lymphocyte from mouse spleen were optimized. The applicability of the cultures to assay for inhibiting and stimulating humoral factors and cytostatics was demonstrated.
D000362:Agar; D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology*; D001853:Bone Marrow; D002455:Cell Division; D046508:Culture Techniques / Q000295:instrumentation / Q000379:methods*; D006098:Granulocytes / Q000166:cytology*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D008264:Macrophages / Q000166:cytology*; D051379:Mice; D013154:Spleen; D013601:T-Lymphocytes / Q000166:cytology*
D000362:Agar