[Measurement of fluorescence decay of substances absorbed by an isolated living cell: an experimental device and early results].
A microfluorometric system allowing the mesurements of fluorescence decay (i.e. fluorescence lifetime) has been built. It will complete the microspectrofluorometric studies of absorbed compounds-polycyclic aromatic hydrocarbons, metabolites of benzo (a) pyrene-by single living cells and allows a better understanding of the intake and metabolisation of these compounds. The preliminary results with benzo (a) pyrene, dibenzocarbazole, pyrene are shown.
D001580:Benzopyrenes / Q000378:metabolism*; D002468:Cell Physiological Phenomena; D003584:Cytological Techniques; D005453:Fluorescence; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013997:Time Factors
D001580:Benzopyrenes
Mesure de la durée de vie de la fluorescence de substances absorbées par une cellule vivante isolée : dispositif expérimental et premiers résultats.