[Isolation and properties of highly purified C1. botulinum toxin type E].
A new method of isolation of highly purified Cl. botulinum toxin of E type from the cultural fluid of strain 188 centrifugates was developed. The method allows to isolate the toxin both in a precursor and in activated forms with a yield of 10--15%. The method includes fractionation by ammonium sulfate, ultrafiltration and subsequent column chromatography on DEAE-cellulose, Sephadex G-200 and DEAE-Sephadex A-50. The preparations were found homogeneous during polyacrylamide gel electrophoresis and immunoprecipitation in agar with antitoxic horse serum. The potential specific toxicity of the preparations is 1--1,2.10(7) DLM/mg of protein. The molecular weight of the toxin is about 160 000; the molar extinction coefficient is equal to 278 nm. The isoelectric point lies around pH 6.0. The highly purified Cl. botulinum toxin of E type was found stable upon storage.
D000818:Animals; D001905:Botulinum Toxins / Q000302:isolation & purification*; D003014:Clostridium botulinum / Q000032:analysis*; D004355:Drug Stability; D005779:Immunodiffusion; D051379:Mice; D008970:Molecular Weight
D001905:Botulinum Toxins
Vydelenie i nekotorye svoĭstva vysokoochishchennogo botulinicheskogo toksina tipa E.