title: [Search for nucleic acid influencing, as well as membrane active, potential cancerostatic fungal metabolites using microbiological and cytological screening methods].
abstract: A prescreening program including microbiological and cytological assays was employed in search of potential cancerostatic antibiotics in crude extracts of mushrooms. The microbiological tests based on agar diffusion techniques consist of prophage induction test and BIP-test. All active compounds selected by these microbiological models are potential inhibitors of nucleic acid metabolism. Cytological assays on leukemia L 1210 cells have been carried out by microscopic examination and by evaluation using an electronic particle counter. Activity was expressed as decrease of the number of single cells caused by agglutination or lysis of cells, changes in cell surface area, dye exclusion, and increase of cell volume. A wide variety of mushrooms was demonstrated to exhibit interesting activities in some of these screening systems. The influence of primary metabolic products of mushrooms on microbiological models was studied additionally. In vivo assays have not yet been accomplished.
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000096:biosynthesis* / Q000494:pharmacology; D001203:Ascomycota / Q000378:metabolism*; D001412:Bacillus subtilis / Q000187:drug effects; D001487:Basidiomycota / Q000378:metabolism*; D002452:Cell Count / Q000187:drug effects; D002460:Cell Line; D003584:Cytological Techniques; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D008828:Microbiological Techniques; D009696:Nucleic Acids / Q000378:metabolism; D013045:Species Specificity
chemical_list: D000903:Antibiotics, Antineoplastic; D009696:Nucleic Acids
vernacular_title: Suche nach Nucleinsäurestoffwechsel-beeinflussenden sowie membranaktiven potentiell cancerostatisch wirksamen Pilzinhaltsstoffen durch mikrobiologische und cytologische Screeningmethoden.