[Differentiation in vitro of chick-embryo skin in presence of histones. Analysis of protein synthesis in toto and of keratin in particular, with the use of labeled precursors].
Six-day limb skin from a chick embryo was cultured in vitro for seven days in a complete medium either supplemented or not with histones. At the end of the incubation period, the chick embryo skin cultured in the absence of histones was found to undergo keratinization, the converse being true for the limbs cultured in the presence of histones. In the latter, when H3-leucine and C14-cystine were added to the medium, a sharp decrease in the labeled amino acid incorporation was found.
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002642:Chick Embryo; D003553:Cystine / Q000378:metabolism; D006657:Histones / Q000494:pharmacology*; D007633:Keratins / Q000096:biosynthesis*; D007930:Leucine / Q000378:metabolism; D014176:Protein Biosynthesis; D012867:Skin / Q000166:cytology / Q000196:embryology* / Q000378:metabolism
D006657:Histones; D003553:Cystine; D007633:Keratins; D007930:Leucine
Studio del differenziamento in vitro della pelle di embrione di pollo in presenza di istoni. Analisi della sintesi proteica in toto e della cheratina in par ticolare con l'uso di precursori marcati.