[A method of separation of antilipoprotein immunoglobulins by chromatography on glass beads (author's transl)].
In this method, under certain experimental conditions, the use of calibrated glass beads permitted the separation of lg with antilipoprotein antibody activity (HLAA). The sera are first precipitated on ammorium sulphate at 25 per cent, 30 per cent and 40 per cent saturation. The precipitates are then submitted to short ultracentrifugation and the product of ultra-entrifugation is fractionnated on a glass bead column, balanced then eluted with an acetate veronal buffer at pH 9. The eluted fraction in peak 1 contains antibodies (IgG or IgA) and albumin. Passive hemagglutination with red cells covered with beta and alpha-lipoproteins, through chromium chloride, together with inhibition of the reaction show that the activity of these Ig antibodies is directed against lipoprotein antigens.
D002846:Chromatography, Affinity / Q000379:methods; D005898:Glass; D006386:Hemagglutination Tests; D006801:Humans; D005779:Immunodiffusion; D007070:Immunoglobulin A / Q000302:isolation & purification; D007074:Immunoglobulin G / Q000302:isolation & purification; D007136:Immunoglobulins / Q000302:isolation & purification*; D008074:Lipoproteins / Q000276:immunology*; D009194:Myeloma Proteins / Q000302:isolation & purification
D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007136:Immunoglobulins; D008074:Lipoproteins; D009194:Myeloma Proteins
Une méthode de séparation des immunoglobulines antilipoprotéines per chromatographie sur billes de verre.