Overlapping pathways for repair of damage from ultraviolet light and chemical carcinogens in human fibroblasts.
DNA excision repair was measured in cultured human fibroblasts after single or dual treatments with ultraviolet radiation, 4-nitroquinoline 1-oxide, or N-acetoxy-2-acetylaminofluorene. Three approaches were used to monitor repair: unscheduled DNA synthesis, measured by autoradiography; repair replication, measured by the incorporation of a density-labeled DNA precursor into repaired regions; and excision of ultraviolet endonuclease-sensitive sites. When a single repair- saturating dose of one of the three carcinogens was administered, little stimulation of unscheduled DNA synthesis or repair replication could be observed by additional treatment with one of the other carcinogens. In no instance was total additivity of repair observed. These observations were confirmed by showing that the excision of endonuclease-sensitive sites produced by ultraviolet damage (i.e., pyrimidine dimers) was inhibited by exposure to 4-nitroquinoline 1-oxide and N-acetoxy-2-acetylaminofluorene. The data indicate that the repair of lesions induced by these substances may have common rate-limiting steps, a conclusion previously indicated by the repair deficiency in xeroderma pigmentosum cells in which a single mutation eliminates the repair of damage caused by each of these agents.
D015112:4-Nitroquinoline-1-oxide / Q000494:pharmacology; D000099:Acetoxyacetylaminofluorene / Q000494:pharmacology; D002273:Carcinogens / Q000494:pharmacology*; D002478:Cells, Cultured; D003043:Cocarcinogenesis; D004260:DNA Repair; D005347:Fibroblasts / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D014466:Ultraviolet Rays
D002273:Carcinogens; D015112:4-Nitroquinoline-1-oxide; D000099:Acetoxyacetylaminofluorene