Biological and artificial ion exchangers: electrical measurements with glass microelectrodes.
Biological (stratum corneum) and artificial (cation-exchange resin beads, Bio-Rad AG 50W-X2) ion exchangers were impaled by glass microelectrodes filled with KCl solution. The electrical potential difference recorded in these structures in reference to the external bathing medium was shown to be dependent on the KCl concentration of both the external and the microelectrode filling solutions. The potentials were interpreted on the grounds of the fixed charge theory of membrane potentials as a consequence of two phase boundary potentials (Donnan potentials), one at the matrix-external solution interface and the other at the matrix-microelectrode solution interface. The contribution of a diffusion component for the recorded potential was considered.
D000818:Animals; D002024:Bufo marinus; D005898:Glass; D066298:In Vitro Techniques; D008564:Membrane Potentials; D008839:Microelectrodes; D011189:Potassium Chloride; D012867:Skin / Q000378:metabolism
D011189:Potassium Chloride