Antigenic make-up of Trypanosoma cruzi culture forms: identification of a specific component.
A comparison of Trypanosoma cruzi water soluble antigens with those of stercorarian and salivarian trypanosomes, and Leishmania using immunoprecipitation in gels and immunoelectrophoresis, with the aid of hyperimmune rabbit serum and heterologous adsorptions showed the following. 1) There is a high complexity of soluble antigens of T. cruzi and T. rangeli. 2) At the intraspecific level our results demonstrated the antigenic stability of T. cruzi when maintained in vitro, and that there was quantitative antigenic consistency of the culture forms of different strains of T. cruzi from diverse geographic and parasite sources. At the interspecific level, the antigenic relationships between T. cruzi and the other Trypanosomatidae were established, as follows: 6/10ths of the antigens are shared by stercorarian species (T. dionisii, T. rangeli); 4/10ths by a salivarian trypanosome (T. brucei); and 3/10ths by Leishmania (L. donovani, L. mexicana). 3) Among the 4/10ths of antigenic components specific to T. cruzi, one component was characterized by its antigenicity and immunogenicity in natural and experimental infections, and in immunization experiments; this component was specific to T. cruzi when compared to the other Trypanosomatidae antigens.
D000818:Animals; D000941:Antigens / Q000032:analysis*; D000939:Epitopes; D006801:Humans; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007891:Leishmania / Q000276:immunology; D012995:Solubility; D014345:Trypanosoma / Q000276:immunology; D014349:Trypanosoma cruzi / Q000276:immunology*; D014867:Water
D000941:Antigens; D000939:Epitopes; D014867:Water