Microimmunodiffusion test for diagnosis of ovine progressive pneumonia.
Two microimmunodiffusion tests (MIDT) for detection and measurement of ovine progressive pneumonia antibody are described. Substrates of various salt concentrations and pH were used to determine the optimal conditions for the tests. In comparisons between two MIDT and one macroimmunodiffusion test, sera from cull ewes were used. The MIDT require less reagents and were more responsive than the macroimmunodiffusion test. After extended incubation of the test materials, results in all three tests were comparable.
D000818:Animals; D000937:Antigen-Antibody Reactions; D005779:Immunodiffusion / Q000379:methods*; D011021:Pneumonia, Progressive Interstitial, of Sheep / Q000276:immunology*; D011233:Precipitin Tests; D012756:Sheep