Original method of showing some constitutive elements of the capillary wall.
The ascorbic acid and the 1-epi-3', 4', 5', 5, 7-pentahydroxy-flavan-3-ol have been located in the blood capillary wall by an histochemical method for electron microscopy. The method consists in an intra-arterial administration of vegetal oxydases specific of the compound to put in evidence. Those oxydases fix themselves on their effectors, and the addition of osmium and lead salts gives co-precipitates opaque to electrons.
D000818:Animals; D001204:Ascorbate Oxidase; D001205:Ascorbic Acid / Q000032:analysis; D002196:Capillaries / Q000032:analysis*; D002200:Capillary Resistance; D004156:Catechol Oxidase; D005419:Flavonoids; D006168:Guinea Pigs; D006651:Histocytochemistry; D006735:Horseradish Peroxidase; D010944:Plants / Q000201:enzymology
D005419:Flavonoids; D004156:Catechol Oxidase; D001204:Ascorbate Oxidase; D006735:Horseradish Peroxidase; D001205:Ascorbic Acid