title: The effect of nalidixic acid, rifampicin and chloramphenicol on the synthesis of phospholipase C in Bacillus cereus.
abstract: The effect of nalidixic acid, rifampicin and chloramphenicol on the synthesis of phospholipase C (EC 3.1.4.3) has been studied in washed Bacillus cereus cells resuspended in nutrient broth. In the absence of inhibitors, the synthesis showed a biphasic pattern. No synthesis of release of enzyme was found in the presence of chloramphenicol. When rifampicin was added, phospholipase C synthesis continued for 10-15 min. Nalidixic acid, at concentrations which inhibited DNA synthesis completely, permitted the synthesis of phospholipase C at the same rate and for a similar length of time as rifampicin.
mesh_terms: D001409:Bacillus cereus / Q000187:drug effects / Q000201:enzymology*; D002701:Chloramphenicol / Q000494:pharmacology*; D009268:Nalidixic Acid / Q000494:pharmacology*; D010740:Phospholipases / Q000096:biosynthesis*; D012293:Rifampin / Q000494:pharmacology*
chemical_list: D009268:Nalidixic Acid; D002701:Chloramphenicol; D010740:Phospholipases; D012293:Rifampin