Redistribution of intrarenal blood flow following ADH administration: lack of inhibition by blockade of prostaglandin in cyclooxygenase.
The effect of prostaglandin synthesis inhibition on the redistribution of renal cortical blood flow in response to antidiuretic hormone (ADH) was examined using radioactive microspheres in water loaded, thiopental-anesthetized dogs. Microsphere injections were made during a control and an ADH infusion period (0.35 mU/kg/min following a 20 mU/kg bolus)  both before and after indomethacin pretreatment (8 mg/kg intravenously). Urinary prostaglandin E2 (PGE2) excretion in each period was measured by gas chromatography-mass spectrometry. ADH caused a marked redistribution of flow toward inner cortical zones from 19 +/- 1 to 25 +/- 2 ml/min (mean +/- SE, p less than 0.01). Fractional flow to inner zones was also significantly increased. Indomethacin pretreatment had no effect on the ADH-induced redistribution (17 +/- 2 vs. 24 +/- 2 ml/min, p less than 0.01), although urinary PGE2 excretion was suppressed by indomethacin by 60%. It is concluded that prostaglandins do not mediate the redistribution of intrarenal blood flow accompanying ADH administration.
D000818:Animals; D016861:Cyclooxygenase Inhibitors; D004285:Dogs; D007213:Indomethacin / Q000494:pharmacology; D007668:Kidney / Q000098:blood supply* / Q000187:drug effects; D007672:Kidney Cortex / Q000098:blood supply / Q000187:drug effects; D008863:Microspheres; D011458:Prostaglandins E / Q000652:urine; D012039:Regional Blood Flow / Q000187:drug effects; D014667:Vasopressins / Q000037:antagonists & inhibitors / Q000494:pharmacology*
D016861:Cyclooxygenase Inhibitors; D011458:Prostaglandins E; D014667:Vasopressins; D007213:Indomethacin