Analysis of RNA secondary structure by photochemical reversal of psoralen crosslinks.
Aminomethyltrioxsalen (AMT), a psoralen, is known to cause interstrand crosslinks in double stranded nucleic acids. We have demonstrated the photochemical reversal of this reaction, and have used this result to develop a method for identification of specific sequences which are adjacent because of RNA secondary structure formation. E. coli 5S rRNA is used as a model system. We isolated and characterized a product that is derived from the stem region of 5S RNA.
D001482:Base Composition; D001483:Base Sequence; D004926:Escherichia coli / Q000032:analysis; D011564:Furocoumarins / Q000528:radiation effects; D009690:Nucleic Acid Conformation; D012329:RNA, Bacterial; D006163:Ribonuclease T1; D014307:Trioxsalen / Q000031:analogs & derivatives / Q000528:radiation effects; D014466:Ultraviolet Rays
D011564:Furocoumarins; D012329:RNA, Bacterial; C018822:aminomethyltrioxsalen; D006163:Ribonuclease T1; D014307:Trioxsalen