title: Argentophil neuronal perikarya and neurofibrils induced by postmortem trauma and hypertonic perfusates.
abstract: Argentophil neuronal perikarya and perikaryal neurofibrils similar to those illustrated in Ramón y Cajal's classical studies have in the present investigation been found to be manifestations of the chromophil neuron. Conclusive evidence of such association was obtained by silver impregnation with the Bodian technique of sections previously stained with cresyl violet. Regardless of the fixative used, silver-impregnated neurofibrils were evident when (1) normal tissues were fixed by immersion or unsuccessfully fixed by perfusion, (2) normal tissues were exposed and touched after death but before perfusion with the fixative, or (3) flow of perfusates was compromised by the effect of an experimental procedure, as well as when (4) a hypertonic saline solution was used in the first perfusate. These cytologic peculiarities were still discernible after 24 h of postmortem autolysis following a delay in removal of the brain or in immersion of the exposed brain in the fixative. After immersion fixation, argentophilia and chromophilia occurred ubiquitously in the brain of the newborn guinea pig; however, argentophil neurofibrils were noted in the absence of chromophil neurons in the brain stem of the newborn rat, rabbit and cat. After fixation by perfusion, perikaryal neurofibrils were not impregnated in either newborn or old animals or in animals with facial nerve transection. Affinity for Congo red or birefringency, exhibited by neurons with marked neurofilbrillary changes in human senile brain atrophy, were absent in the present material. On the basis of the current light-microscopic observations, it is concluded that argentophilia of neuronal perikarya and perikaryal neurofibrils is another manifestation of the chromophil neuron induced by postmortem trauma and of the ocellate neuron elicited by perfusion with hypertonic saline.
mesh_terms: D000367:Age Factors; D000818:Animals; D001921:Brain / Q000166:cytology / Q000473:pathology; D002415:Cats; D004285:Dogs; D005404:Fixatives; D006168:Guinea Pigs; D008853:Microscopy; D009454:Neurofibrils / Q000187:drug effects / Q000648:ultrastructure*; D009474:Neurons / Q000187:drug effects / Q000648:ultrastructure*; D010477:Perfusion; D011180:Postmortem Changes; D011689:Purkinje Cells / Q000473:pathology; D011817:Rabbits; D051381:Rats; D012462:Saline Solution, Hypertonic / Q000494:pharmacology*; D012834:Silver; D012965:Sodium Chloride / Q000494:pharmacology*; D013045:Species Specificity; D013116:Spinal Cord / Q000502:physiology; D013194:Staining and Labeling
chemical_list: D005404:Fixatives; D012462:Saline Solution, Hypertonic; D012834:Silver; D012965:Sodium Chloride