Elution of chicken Ig from fixed target cells.
A method is described for preparing fixed chicken peripheral blood cells (PBC) for absorption of allo-antibodies and their subsequent elution. Glutaraldehyde fixation was satisfactory, and with 8 M urea elution haemagglutinating activity was preserved.
D000327:Adsorption; D000818:Animals; D000918:Antibody Specificity; D001773:Blood Cells; D002645:Chickens; D005557:Formaldehyde; D005976:Glutaral; D006386:Hemagglutination Tests; D007074:Immunoglobulin G; D007158:Immunologic Techniques / Q000379:methods*; D007518:Isoantibodies; D013861:Thiocyanates / Q000494:pharmacology
D007074:Immunoglobulin G; D007518:Isoantibodies; D013861:Thiocyanates; D005557:Formaldehyde; D005976:Glutaral