title: Possible roles for microtubules and microfilaments in ADH action on toad urinary bladder.
abstract: Intramembranous particle aggregates in the luminal membrane of toad bladder granular cells after vasopressin stimulation have been found to correlate closely and specifically with induced alterations of water permeability. Roles for microtubules and microfilaments in mediating the latter response have been proposed on the basis of studies involving colchicine and cytochalasin B, respectively. In the present investigation the effects of these agents on both initiating and sustaining vasopressin-induced osmotic water flow and the particle aggregation phenomenon were studied. The results indicate that during initiation the aggregation and water flow responses to vasopressin are each colchicine- and cytochalasin B-sensitive and that these sensitivities can be wholly additive. However, after full vasopressin stimulation is established, the same responses demonstrate sensitivity only to cytochalasin B, not to colchicine. The findings, therefore, suggest that microtubules and microfilaments may be independently necessary for the initiation of the aggregation and water flow responses to vasopressin, and that microfilaments, but not microtubules, are required for their maintenance.
mesh_terms: D000818:Animals; D002024:Bufo marinus; D003078:Colchicine / Q000494:pharmacology; D003571:Cytochalasin B / Q000494:pharmacology; D003593:Cytoplasm / Q000502:physiology*; D003599:Cytoskeleton / Q000502:physiology*; D008870:Microtubules / Q000502:physiology*; D009994:Osmolar Concentration; D001743:Urinary Bladder / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D014667:Vasopressins / Q000494:pharmacology*; D014867:Water / Q000378:metabolism
chemical_list: D014867:Water; D014667:Vasopressins; D003571:Cytochalasin B; D003078:Colchicine