[Arguments in favor of the extramitochondrial origin of the acetyl radical of acetylcholine synthetized in rat striatum synaptosomes].
Synaptosomes prepared from Rat striatum were loaded with (2-14C) pyruvate at 0 degree C, in the course of a 1 hr. preincubation. When temperature was raised to 37 degrees C, the intra-synaptosomal (2-14C) pyruvate gave rise to 14CO2 but not to (14C) ACh. These results exclude a mitochondrial origin of the acetyl moiety of ACh.
D000085:Acetates / Q000378:metabolism*; D000109:Acetylcholine / Q000096:biosynthesis*; D000818:Animals; D003342:Corpus Striatum / Q000378:metabolism*; D007700:Kinetics; D008928:Mitochondria / Q000378:metabolism; D011773:Pyruvates / Q000378:metabolism; D051381:Rats; D013347:Subcellular Fractions / Q000378:metabolism; D013574:Synaptosomes / Q000378:metabolism*
D000085:Acetates; D011773:Pyruvates; D000109:Acetylcholine
Arguments en faveur de l'origine extramitochondriale du radical acétyle de l'acétylcholine synthétisée dans des synaptosomes du striatum de rat.