Probes of beta-galactosidase structure with antibodies. Reaction of anti-peptide antibodies against native enzyme.
Antibodies were prepared against 18 tryptic and cyanogen bromide peptides from beta-galactosidase ranging in size from 15 to 96 amino acid residues representing more than 80% of the polypeptide chain. They were tested for binding capacity and affinity toward their homologous antigens and toward the whole native protein. Nine antisera bound to beta-galactosidase; these had been raised against certain peptides from the central and carboxyl-terminal regions of the poly-peptide chain. Based on these results a preliminary model of the three-dimensional structure of the folded protein is suggested.
D000906:Antibodies; D000915:Antibody Affinity; D000937:Antigen-Antibody Reactions; D001666:Binding Sites, Antibody; D003488:Cyanogen Bromide; D005696:Galactosidases / Q000276:immunology*; D010446:Peptide Fragments / Q000276:immunology; D011487:Protein Conformation; D014357:Trypsin; D001616:beta-Galactosidase / Q000276:immunology*
D000906:Antibodies; D010446:Peptide Fragments; D005696:Galactosidases; D001616:beta-Galactosidase; D014357:Trypsin; D003488:Cyanogen Bromide