[Comparison of three anti-nucleus antibody detection methods in different pathologies (author's transl)].
Anti-nucleus antibodies were detected in the blood sera of 232 patients of different pathologies by three diverse methods: a morphological method, and indirect immunofluorescent method and a radioimmunologic method. The results demonstrated the importance of the radioimmunologic method since this method displays anti-DNA antibodies which are specific in the active stage of lupus erythematosus (LE). Forthermore, the immunofluorescent method proved to be highly sensitive; and the morphological method can reveal different aspects of the LE phenomenon (such as nucleus phagocytosis) which can be indicative of an auto-aggressive process. Our survey indicates that simultaneous use of the three methods is advisable.
D000974:Antibodies, Antinuclear / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007244:Infectious Mononucleosis / Q000276:immunology; D008107:Liver Diseases / Q000276:immunology; D008171:Lung Diseases / Q000276:immunology; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D009369:Neoplasms / Q000276:immunology; D009504:Neutrophils; D011863:Radioimmunoassay; D013587:Syphilis / Q000276:immunology
D000974:Antibodies, Antinuclear
Confronto di tre metodi nella ricerca di anticorpi anti-nucleo in diverse forme morbose.