Study of calcium dipicolinate release during bacterial spore germination by using a new, sensitive assay for dipicolinate.
The release of calcium and dipicolinic acid from spores of Bacillus megaterium KM during L-alanine-induced triggering of germination has been studied using a new, simple, and rapid assay for dipicolinic acid capable of detecting a concentration of 0.5 micron. The release of both calcium and dipicolinate started within seconds of exposure of the spores to L-alanine, thus preceding other measurable changes associated with germination. From the earliest times, the two substances were released in equimolar quantities, although later in germination calcium predominated.
D001410:Bacillus megaterium / Q000254:growth & development / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D006358:Hot Temperature; D010848:Picolinic Acids / Q000032:analysis / Q000378:metabolism*; D013057:Spectrum Analysis / Q000379:methods; D013171:Spores, Bacterial / Q000254:growth & development / Q000378:metabolism
D010848:Picolinic Acids; D002118:Calcium