[Isolation and study of influenza A viruses in different cell cultures].
The usefulness of some cells cultures (BSC-I, VERO, MDSK) for isolation and study of reproduction of influenza A viruses was explored. Out of 50 clinical specimens examined, in 16 cases the virus was isolated both in chick embryos and in MDSK cell cultures. MDSK cultures were found to be highly sensitive to influenza A viruses, which produced cytopathic effect and hemagglutinin accumulation. The plaques formed by freshly isolated strains under an agar overlay containing trypsin were markedly polymorphous. MDSK cells may be recommended for use in virus isolation studies. Examinations of virion morphology revealed their diversity both in size and shape.
D000818:Animals; D002642:Chick Embryo; D004285:Dogs; D000882:Haplorhini; D006387:Hemagglutination, Viral; D006801:Humans; D009980:Influenza A virus / Q000302:isolation & purification*; D007251:Influenza, Human / Q000382:microbiology; D007668:Kidney; D008854:Microscopy, Electron; D010948:Viral Plaque Assay; D014776:Virus Cultivation
Vydelenie i izuchenie virusov grippa A na raznykh kletochnykh kul'turakh.