[Transfer of a bacterial gene using phage lambda transfecting DNA].
A new amber mutation of phage with the gene coding synthesis of beta-galactosidase was received by recombination. With the help of transfection DNA isolated from this phage the transfer of the gene coding the beta-galactosidase synthesis to the recipient phage-resistant E. coli cell was realized. The suggested model can be used for the gene transfer to the recipient phage-resistant cells or other species of bacteria with transfection DNA.
D003090:Coliphages / Q000235:genetics*; D003433:Crosses, Genetic; D004279:DNA, Viral / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D009154:Mutation; D011995:Recombination, Genetic; D014162:Transfection; D001616:beta-Galactosidase / Q000235:genetics
D004279:DNA, Viral; D001616:beta-Galactosidase
Perenos bakterial'nogo gena s pomoshch'iu transfitsiruiushcheĭ DNK faga lambda.