title: [Coordinate regulation of riboflavin permease and alpha-glucosidase synthesis in the yeast Pichia guilliermondii].
abstract: Riboflavin permease of the yeast Pichia guilliermondii appear to be inducible transport system. Its synthesis is induced by sucrose, maltose, alpha-methyl-D-glocoside, melizitose and raffinose, but not by D-glucose, trehalose or cellobiose. The synthesis of riboflavin permease in the presence of sucrose of maltose is depressed by cycloheximide, actinomycin D and 8-hydroxyquinoline. These results suggest that the synthesis of riboflavin permease is regulated on the transcription level. The inducers of riboflavin permease are also able to induce the synthesis of alpha-glucosidase. The mutants have been selected in which the synthesis of riboflavin permease occurs constitutively; the synthesis of alpha-glucosidase in the mutants is also constitutive. Growing of the yeast in a medium with high content of glucose results in a parallel decrease of the riboflavin permease and alpha -- glucosidase activities. These data are indicative of corrdinate regulation of riboflavin permease and alpha -- glucosidase in P. guilliermondii. Suboptimal or excessive content of vitamin B2 in the medium does not affect the level of riboflavin permease in this yeast species.
mesh_terms: D001203:Ascomycota / Q000201:enzymology*; D002241:Carbohydrates / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D005959:Glucosidases / Q000096:biosynthesis*; D007700:Kinetics; D026901:Membrane Transport Proteins / Q000096:biosynthesis*; D015125:Oxyquinoline / Q000494:pharmacology; D010843:Pichia / Q000187:drug effects / Q000201:enzymology*; D012256:Riboflavin; D000520:alpha-Glucosidases / Q000096:biosynthesis*
chemical_list: D002241:Carbohydrates; D026901:Membrane Transport Proteins; D003609:Dactinomycin; D015125:Oxyquinoline; D003513:Cycloheximide; D005959:Glucosidases; D000520:alpha-Glucosidases; D012256:Riboflavin
vernacular_title: Koordinirovannaia reguliatsiia sinteza piboflavinpermeazy i alf'a-gliukozidazy u drozhzhei Pichia guilliermondii.