[Bactericidal antibody micro-assay for detection of antibodies against meningococci (author's transl)].
The best way to detect protective antibodies against meningococci in human serum seems to prove its bactericidal activity. For this a bactericidal antibody assay was described by WHO. For this test, however, a high amount of baby rabbit-serum is required. Therefore a modification was introduced by reducing the test volumes, performing the test in microtiterplates and using TTC (triphenyl tetracolium chloride), as a growth indicator that changes from colourless to purple red in the presence of growing meningococci. The end point of serum titration is the last well containing colourless medium.
D000907:Antibodies, Bacterial / Q000032:analysis*; D007158:Immunologic Techniques; D008832:Microchemistry; D009345:Neisseria meningitidis / Q000276:immunology*
D000907:Antibodies, Bacterial
Bakterizidie-Test im Mikrosystem zum Nachweis von Antikörpern gegen Meningokokken.