Raising of antisera to protein hormones without using Freund's adjuvant.
A new method has been developed to produce antiserum to protein hormones without the use of Freund's complete adjuvant. Antiserum to human chorionic gonadotropin (hCG) was elicited by injecting rabbits with 6 subcutaneous injections of sheep red blood cells (SRBC) coated with hCG. The antiserum produced was observed to be both serologically and biologically active. A small quantity of the hormone (15--20 IU/injection) sufficed to produce hyperimmune sera. This could ensure an overall economy if large amounts of the antisera are required for clinical and diagnostic purposes.
D000917:Antibody Formation; D000941:Antigens / Q000008:administration & dosage; D006063:Chorionic Gonadotropin / Q000276:immunology*; D006385:Hemagglutination Inhibition Tests; D006386:Hemagglutination Tests; D007106:Immune Sera; D005779:Immunodiffusion; D008722:Methods; D011233:Precipitin Tests; D011863:Radioimmunoassay
D000941:Antigens; D006063:Chorionic Gonadotropin; D007106:Immune Sera