[Disappearance of the gliofibrillar protein (GFA) during the cultivation of glioblastoma cells].
Absence of GFA in established cell lines from glioblastomas promped us to look for this protein from the first replication of malignant cells in vitro and in following subcultrues. Our results show that GFA disappeared during the first twelve replications, often before the fifth replication. No correlation was observed with a morphological transformation of the cells.
D000951:Antigens, Neoplasm / Q000032:analysis; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D005910:Glioma / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D009363:Neoplasm Proteins / Q000302:isolation & purification* / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000302:isolation & purification* / Q000378:metabolism; D009457:Neuroglia / Q000032:analysis
D000951:Antigens, Neoplasm; D009363:Neoplasm Proteins; D009419:Nerve Tissue Proteins
Disparition de la protéine gliofibrillaire (GFA) au cours de la culture de cellules de glioblastomes.