Transfer of RP4 and R68.45 factors to Rhizobium.
Two R factor were introduced by conjugation into Rhizobium trifolii and Rhizobium meliloti strains at a frequency of 10(-5) to 10(-6). Plasmids RP4 from Escherichia coli J53 and R68.45 from Pseudomonas aeruginosa PAO.25 were maintained stably in Rhizobium hosts and could be retransferred to other Rhizobium recipients. Some of the transconjugants were able to mobilize chromosome and transfer his or met genes in intra-, and interspecies matings.
D003227:Conjugation, Genetic; D046508:Culture Techniques; D004926:Escherichia coli / Q000235:genetics; D010957:Plasmids; D011550:Pseudomonas aeruginosa / Q000235:genetics; D011815:R Factors; D012231:Rhizobium / Q000235:genetics*