title: The nucleotide sequence of human tRNAGly (anticodon GCC).
abstract: The sequence of tRNAGCCGly from human placenta was determined by recently developed postlabeling techniques. The tRNA was digested completely with RNases T1 and A in the presence of alkaline phosphatase, the oligonucleotides were 3'-terminally (3H)-labeled, mapped on PEI-cellulose thin layers, isolated, and sequenced by methods based on base-specific cleavages. Overlaps were obtained by readout sequencing techniques on polyacrylamide gels and PEI-cellulose thin layers. The thin-layer readout technique was used also to locate and identify modified nucleotides. The primary structure was found to exhibit a large degree of homology (94.6%) with silkworm tRNAGCCGly but only 67.6% homology with human tRNACCCGly.
mesh_terms: D000926:Anticodon; D001483:Base Sequence; D005260:Female; D005998:Glycine; D006801:Humans; D009843:Oligoribonucleotides / Q000032:analysis; D010920:Placenta / Q000032:analysis*; D011247:Pregnancy; D012343:RNA, Transfer / Q000302:isolation & purification; D006163:Ribonuclease T1; D012260:Ribonucleases
chemical_list: D000926:Anticodon; D009843:Oligoribonucleotides; D012343:RNA, Transfer; D012260:Ribonucleases; D006163:Ribonuclease T1; D005998:Glycine