[Purification and characterization of the free secretory piece of human colostrum (author's transl)].
The free secretory piece is isolated from human colostrum by gel filtration and ion-exchange chromatography in high yield (200 mg/l colostrum). DEAE-Cellulose chromatography separates the free secretory piece in two fractions which are electrophoretically distinct, but otherwise have the same characteristics, like molecular weight, antigenic determinants, N-terminal sequence, peptide map and amino acid composition. It was therefore concluded that the protein part of the secretory piece is homogenous.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D003126:Colostrum / Q000032:analysis*; D000939:Epitopes; D005260:Female; D006801:Humans; D007128:Immunoglobulin Fragments / Q000302:isolation & purification; D008894:Milk Proteins / Q000302:isolation & purification; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis; D011247:Pregnancy; D012635:Secretory Component / Q000302:isolation & purification
D000596:Amino Acids; D000939:Epitopes; D007128:Immunoglobulin Fragments; D008894:Milk Proteins; D010446:Peptide Fragments; D012635:Secretory Component
Reinigung und Charakterisierung der freien sekretorischen Komponente aus Human-Kolostrum.