title: Characterization of Pseudomonas aeruginosa mutants deficient in the establishment of lysogeny.
abstract: Mutants of Pseudomonas aeruginosa with impaired ability to establish a lysogenic relationship with temperate bacteriophage (Les-) have been isolated. These les mutations map to two areas of the P. aeruginosa chromosomal map as determined by conjugational and transductional analyses. Two phenotypic classes of Les- mutants were identified. One class of mutations has pleiotropic effects on DNA metabolism. These mutants are unable to recombine genetic material acquired as a result of either conjugation or transduction (Rec-). In addition, the ability of these Les- Rec- mutants to repair UV-induced damage to bacteriophage is reduced (host-cell reactivation deficient, Hcr-). Mutants of the second class are Les-, Rec+, and Hcr+.
mesh_terms: D001435:Bacteriophages / Q000254:growth & development; D002874:Chromosome Mapping; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000378:metabolism; D008242:Lysogeny; D009154:Mutation; D010641:Phenotype; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000378:metabolism; D011995:Recombination, Genetic
chemical_list: D004269:DNA, Bacterial