title: A histochemical study about the influence of lytic enzymes on plasma membrane enzyme activities in rat liver and kidney.
abstract: 1. The effect of lipolytic, glycolytic and proteolytic enzymes on the activities of plasma membrane enzyme activities in rat liver and kidney has been investigated by a pretreatment of tissue sections with the lytic enzymes. 2. The action of the proteolytic enzymes causes a very strong decrease of leucyl-beta-naphthylamidase activity, whereas the activities of ATP-ase, 5'-nucleotidase and alkaline phosphatase show a lesser decrease. This indicates a different membrane anchorage of leucyl-beta-naphthylamidase as compared to that of the phosphatases. 3. Treatment with glycolytic enzymes results in a decrease of 5'-nucleotidase and ATP-ase activity, whereas liver alkaline phosphatase and leucyl-beta-naphthylamidase show an increase in activity. 4. Treatment with phospholipase C gives about the same results. The very strong decrease of 5'-nucleotidase activity indicates a great dependence on phospholipids.
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D007668:Kidney / Q000201:enzymology*; D007931:Leucyl Aminopeptidase / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D008297:Male; D009439:Neuraminidase / Q000494:pharmacology; D009708:Nucleotidases / Q000378:metabolism; D010447:Peptide Hydrolases / Q000494:pharmacology; D010740:Phospholipases / Q000494:pharmacology; D051381:Rats; D001616:beta-Galactosidase / Q000494:pharmacology
chemical_list: D010740:Phospholipases; D009708:Nucleotidases; D000469:Alkaline Phosphatase; D009439:Neuraminidase; D001616:beta-Galactosidase; D010447:Peptide Hydrolases; D007931:Leucyl Aminopeptidase