Histochemical detection of carbonic anhydrase with diemthylaminonaphthalene-5-sulfonamide.
A new specific method for the detection of carbonic anhydrase, EC 4.2.1.1, in tissues is described. The reaction of carbonic anhydrase with dimethylaminonaphthalene-5-sulfonamide (DNSA) forms a highly fluorescent complex. The specificity of the method is proved by the quenching of this fluorescence with ethoxzolamide (6-ethoxybenzothiazole-5-sulfonamide). The difference in the wavelength makes it possible to absorb the fluorescence of the unbound dimethylaminonaphthalene-5-sulfonamide by filters. Kidney, proventriculus, and bone from chicken have been examined. Carbonic anhydrase has been detected in the cytoplasm of the columnar lining cells, proximal tubule cells, and osteoclasts.
D000818:Animals; D001842:Bone and Bones / Q000201:enzymology / Q000648:ultrastructure; D002256:Carbonic Anhydrases / Q000032:analysis*; D002645:Chickens; D003619:Dansyl Compounds; D006651:Histocytochemistry; D007668:Kidney / Q000201:enzymology / Q000648:ultrastructure; D008856:Microscopy, Fluorescence / Q000379:methods; D013050:Spectrometry, Fluorescence / Q000379:methods
D003619:Dansyl Compounds; D002256:Carbonic Anhydrases