Immunochemical characterization of casein from rabbit mammary gland.
1. Excellent precipitating antibodies to rabbit recombined casein polypeptides were obtained in a sheep after 8 weeks of immunization with rabbit recombined polypeptides coupled to Sepharose-albumin. 2. The antiserum was assessed for specificity by several immunochemical techniques and was monospecific when tested against acid-precipitated casein, recombined casein and extracts of lactating rabbit mammary tissue. 3. A specific anti-casein immunoglobulin fraction was prepared by immunoadsorption of the antiserum by using Sepharose-recombined casein as immunoadsorbent. 4. The specific anti-casein immunoglobulin was used to prepare a Sepharose-anti-casein immunoadsorbent for the isolation of casein from extracts of rabbit mammary tissue.
D000818:Animals; D000918:Antibody Specificity; D002364:Caseins / Q000276:immunology* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007106:Immune Sera; D005779:Immunodiffusion; D007123:Immunoelectrophoresis, Two-Dimensional; D007164:Immunosorbents; D007774:Lactation; D008321:Mammary Glands, Animal / Q000032:analysis*; D011247:Pregnancy; D011817:Rabbits
D002364:Caseins; D007106:Immune Sera; D007164:Immunosorbents