Infectivity suppressing and virus-binding activities of a membrane material isolated from tobacco leaves.
TMV binding substance (R) was isolated from a tobacco leaf membrane fraction and was purified by extraction with organic solvents and by column chromatography. Experimental results suggest that the binding of R with TMV results in inactivation of TMV. When tobacco leaves were inoculated with the R-TMV complex, it was found that the formation of polysome containing infecting viral RNA was inhibited. Model experiments showed that the mode of R-TMV adsorption to the membrane is different from that of TMV adsorption and that stripping of coat protein from TMV by SDS was inhibited by R. A possible explanation for the mechanism of this inhibition by R is that the R-TMV complex follows a pathway which does not lead to establishment of infection. Although less efficient, R was still active when it was applied after virus inoculation. Due to its affinity to coat protein, R might also interfere with a later process of viral multiplication.
D000327:Adsorption; D002462:Cell Membrane / Q000032:analysis / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D010947:Plants, Toxic; D011991:Receptors, Virus / Q000302:isolation & purification / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology; D014026:Nicotiana / Q000032:analysis / Q000382:microbiology*; D014027:Tobacco Mosaic Virus / Q000254:growth & development / Q000378:metabolism*; D005719:gamma-Globulins / Q000494:pharmacology
D011991:Receptors, Virus; D005719:gamma-Globulins; D012965:Sodium Chloride