[Experimental investigations on the regeneration of aortic endothelial cells. Automatic and visual evaluation of autoradiograms (author's transl)].
Autoradiographic studies on plane preparations using 3H-thymidine and pulse cytophotometric investigations on scrape preparations of aortic endothelium from 34 rabbits have brought the following results: By means of multiple 3H-thymidine-injections the number of labelled endothelial cells is higher in young growing animals than in older rabbits; pulse cytophotometrically we did not find any indication of polyploidization processes; from that we conclude a decrease of the growth fraction with advancing age. In comparison to visual counting the automatic image analysis is a highly effective method for the evaluation of autoradiograms. After multiple 3H-thymidine applications the mean blacking intensity of the labelled cell nuclei is lower in 2 months old animals than in 12 months old rabbits; that may mean an increase in the S-phase duration of the proliferating endothelial cells of the older animals. In the area with branching sites there are more 3H-thymidine-labelled endothelial cells than in the neighbouring vascular segments. The significance of these findings for the preferential incidence of arteriosclerosis in this area is discussed.
D000367:Age Factors; D000818:Animals; D001011:Aorta / Q000502:physiology*; D001345:Autoradiography / Q000379:methods; D002452:Cell Count; D002455:Cell Division; D004727:Endothelium / Q000166:cytology; D011817:Rabbits; D012038:Regeneration; D013936:Thymidine; D014316:Tritium
D014316:Tritium; D013936:Thymidine
Experimentelle Untersuchungen über die Regeneration der Aortenendothelzellen. Automatische und visuelle Auswertung von Autoradiogrammen.