A Pseudomonas pyocin typing method applicable to the clinical laboratory.
A new pyocin typing method for the identification of specific strains of Pseudomonas aeruginosa is described. The method involves a bilayer agar technique in which the pyocin produced by the organism diffuses from the top agar layer number 2 through a filter paper into the bottom agar layer number 1. The filter paper permits the removal of agar layer number 2 and exposes layer number 1 which is then streaked with the indicator strains. Pyocin typing of fifty clinical isolates using both the bi-layer method and the scrap-chloroform reference method of Gillies and Govan produced identical typing results. A total of 375 clinical isolates has been typed by this inexpensive and time-saving bi-layer agar method.
D000362:Agar; D001430:Bacteriocins / Q000145:classification*; D001431:Bacteriological Techniques; D011550:Pseudomonas aeruginosa / Q000145:classification* / Q000378:metabolism; D011709:Pyocins / Q000145:classification* / Q000378:metabolism
D001430:Bacteriocins; D011709:Pyocins; D000362:Agar