Purification of human liver microsomal epoxide hydrase. Differences in the properties of the human and rat enzymes.
Human liver microsomal epoxide hydrase has been highly purified to a specific activity (570 to 620 nmol/min/mg of protein) comparable to that of the rat enzyme using styrene oxide as substrate. Like the purified rat liver microsomal epoxide hydrase, the human enzyme has a minimum molecular weight of 49,000 as determined by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate and exhibits broad substrate specificity toward a variety of alkene and arene oxides. Despite these similarities, the human and rat enzymes are different proteins as judged by their immunochemical properties as well as their relative catalytic activities toward certain substrates.
D000818:Animals; D004789:Enzyme Activation; D004851:Epoxide Hydrolases / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D005779:Immunodiffusion; D007527:Isoenzymes / Q000378:metabolism; D007700:Kinetics; D008862:Microsomes, Liver / Q000201:enzymology*; D051381:Rats; D013045:Species Specificity; D013379:Substrate Specificity
D007527:Isoenzymes; D004851:Epoxide Hydrolases