Subpopulations of human peripheral blood cells: analysis of granulocytic progenitor cells by flow cytometry and immunologic surface markers.
Normal human peripheral blood cells were separated into different populations based upon isopycnic sedimentation, E rosetting, and EAC rosetting. Each population was characterized according to morphology, surface markers, granulocytic colony formation in semi-solid media, and stainable RNA content by acridine orange (AO) flow cytometry. These techniques enrich for a population of cells that is characterized by a lymphoid morphology, a high granulocytic-macrophage progenitor cell cloning efficiency, a lack of surface markers, and a high stainable RNA content not found in the other two populations of peripheral blood lymphocytes (T cells and B cells). The stainable RNA content serves as a new metabolic marker for the population of cells in which the preponderance of granulocytic progenitor cells reside.
D001402:B-Lymphocytes / Q000166:cytology; D001773:Blood Cells / Q000032:analysis / Q000166:cytology; D002469:Cell Separation; D002999:Clone Cells / Q000032:analysis; D003584:Cytological Techniques / Q000379:methods*; D005453:Fluorescence; D006098:Granulocytes / Q000166:cytology*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D008214:Lymphocytes / Q000032:analysis; D012313:RNA / Q000032:analysis; D011947:Receptors, Antigen, B-Cell / Q000276:immunology*; D013194:Staining and Labeling; D013601:T-Lymphocytes / Q000166:cytology
D011947:Receptors, Antigen, B-Cell; D012313:RNA