Determination of small differences in buoyant density of macromolecules by analytical density gradient equilibrium centrifugation.
A sensitive method is proposed for the determination of small differences between the buoyant densities of different species of monodisperse macromolecules by analytical density gradient equilibrium centrifugation. The procedure involves the measurement at sedimentation equilibrium of the bandwidths of the concentration distribution of the separate macromolecules and of a mixture of the different species. The difference in buoyant densities can then be estimated from the difference between the bandwidths.
D000818:Animals; D001156:Artemia; D001704:Biopolymers; D002499:Centrifugation, Density Gradient / Q000379:methods; D046911:Macromolecular Substances; D008433:Mathematics; D009698:Nucleoproteins / Q000032:analysis; D012313:RNA / Q000032:analysis; D012261:Ribonucleoproteins / Q000032:analysis; D012270:Ribosomes / Q000032:analysis
D001704:Biopolymers; D046911:Macromolecular Substances; D009698:Nucleoproteins; D012261:Ribonucleoproteins; D012313:RNA