Quantitative aspects of the Naphthol Yellow S staining for proteins studied in a model system of polyacrylamide films and in isolated rat liver cells and nuclei.
After staining with Naphthol Yellow S (NYS) at optimal conditions of pH (2.8), the protein content of rat liver cells isolated by means of a collagenase perfusion technique was found to be cytophotometrically immeasurable, because of too high local dye absorbances. In order to lower the absorption values, techniques to flatten the cells, off-peak measurements and NYS staining at non-optimal pH levels were applied. With polyacrylamide model films incorporated with albumin, the reliability of off-peak measurements and the quantitative aspects of the modified protein staining procedures have been investigated. It was found that NYS is a quantitative protein stain not only at pH 2.8, but also at pH 2.0, 3.5 and 4.0 respectively. Off-peak measurements can also produce quantitative results. In cases of a combined Feulgen-NYS staining, the Feulgen-DNA values were not significantly influenced by any of the NYS staining procedures.
D000180:Acrylic Resins; D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure*; D055598:Chemical Phenomena; D002621:Chemistry; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000166:cytology*; D009282:Naphthalenesulfonates; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D013194:Staining and Labeling / Q000379:methods*; D000099166:Polyacrylamides
D000180:Acrylic Resins; D009282:Naphthalenesulfonates; D011506:Proteins; D000099166:Polyacrylamides; C009824:naphthol yellow