Amino acid sequence of beta-galactosidase. VII. Isolation of the 24 cyanogen bromide peptides.
All of the 24 cyanogen bromide peptides of beta-galactosidase have been isolated in pure form. Of these 8 ranged in size from 2 to 5 residues and were purified by paper electrophoresis. The 16 large peptides, from 23 to 119 residues, were chromatographed at pH 5.0 on a carboxymethyl-cellulose column in 0.02 M ammonium acetate buffer containing 8 M urea. A number of peptides were obtained in pure from following Sephadex G-50 or G-75 gel filtration. Others were separated on sulfopropyl-Sephadex or diethyl-(2-hydroxylpropylaminoethyl)-Sephadex. There large peptides were obtained in over 50% yield and several others were obtained in more than 25% yield.
D000595:Amino Acid Sequence; D003488:Cyanogen Bromide; D004926:Escherichia coli / Q000201:enzymology*; D005696:Galactosidases; D010446:Peptide Fragments / Q000032:analysis; D001616:beta-Galactosidase
D010446:Peptide Fragments; D005696:Galactosidases; D001616:beta-Galactosidase; D003488:Cyanogen Bromide