[Kinetics of decrease and apparent transformation of fluorescence spectra of benzo(a)pyrene absorbed by living cells: case of lymphocytes and macrophages].
A home made microspectrofluorimeter is used in order to follow the decrease of fluorescence intensity of Benzo(a)Pyrene after its absorption by single living cells. The kinetics look to be a first order one; fluorescent metabolite can be detected when peritoneal macrophages of Mice are used but not with human periferic lymphocytes pretreated with mitogens.
D000042:Absorption; D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D001580:Benzopyrenes / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D008214:Lymphocytes / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods; D013050:Spectrometry, Fluorescence / Q000379:methods
D001580:Benzopyrenes
Cinétiques de décroissance et de transformation apparente du spectre de fluorescence du Benzo(a)Pyrène absorbé par des cellules vivantes: cas de lymphocytes et de macrophages.