[Some physico-chemical properties of the compact chromosome of Ananbaena variabilis].
It was shown that total DNA of the blue-green algae A. variabilis can be isolated as a compact particle with the sedimentation constant of 2700--2900S by lysis of the cells with the TritonX-100--sodium deoxycholate mixture in 1.0 M NaCl. The structure isolated is sensitive to ribonuclease and the hydrodynamic shift. It was found that within a compact chromosome A. variabilis DNA is circular and negatively superhelicized. The density of superturns of the DNA in 0,2 M NaCl at 20 degrees C is 0,061, which corresponds to one negative turn per 165 pairs of DNA bases.
D002875:Chromosomes / Q000032:analysis*; D000458:Cyanobacteria / Q000032:analysis* / Q000166:cytology; D004247:DNA / Q000032:analysis*; D004270:DNA, Circular / Q000032:analysis; D008970:Molecular Weight; D009690:Nucleic Acid Conformation
D004270:DNA, Circular; D004247:DNA
Nekotorye fiziko-khimicheskie kharakteristiki kompaktnoĭ khromosomy Anabaena variabilis.