title: Ribonucleic acid polymerase mutation affecting glutamate synthase activity in and sporulation of Bacillus subtilis.
abstract: Spore formation of 15 rifampin-resistant (Rifr) mutants of Bacillus subtilis strain 168 was examined. As a pleiotropic effect of a Rifr mutation, glutamate synthase activity was lost in these mutants. Twelve of the 15 mutants examined formed as many spores as the parent, but the remaining 3 formed significantly fewer (1%) spores. One of the latter mutants characterized further (RF301) was blocked in its sporulation process at stage 0. Thus, it was concluded that a certain modification of ribonucleic acid polymerase may affect specifically the gene expression of glutamate synthase and also the sporulation process at the initial stage.
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics / Q000502:physiology*; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D005796:Genes; D005970:Glutamate Synthase / Q000378:metabolism*; D009154:Mutation; D012293:Rifampin / Q000494:pharmacology; D013171:Spores, Bacterial / Q000502:physiology; D000637:Transaminases / Q000378:metabolism*
chemical_list: D005970:Glutamate Synthase; D000637:Transaminases; D012321:DNA-Directed RNA Polymerases; D012293:Rifampin