An improved method for selecting T-cell hybridomas by the fluorescence-activated cell sorter.
A two-step sorting procedure with the fluorescence-activated cell sorter (FACS) is described to select T cell hybridomas from cell populations obtained from the fusion of activated T cells and T- lymphoma cells. Cells are first sorted on the basis of differences in cell-surface antigens. After a period of growth in culture, the cells are then stained with the DNA-labeling dye dis-benzimidazole 33258 Hoechst and sorted on the basis of their DNA content. From 15 to 25% of the resulting cell clones, growing out of limiting dilutions, were hybrid lines as judged by chromosome analysis.
D000818:Animals; D000954:Antigens, Surface; D006690:Bisbenzimidazole; D002460:Cell Line; D002469:Cell Separation / Q000379:methods*; D004247:DNA / Q000378:metabolism; D006651:Histocytochemistry; D006822:Hybrid Cells / Q000276:immunology* / Q000378:metabolism; D008223:Lymphoma / Q000276:immunology / Q000473:pathology; D051379:Mice; D008856:Microscopy, Fluorescence; D013194:Staining and Labeling; D013601:T-Lymphocytes / Q000276:immunology
D000954:Antigens, Surface; D004247:DNA; D006690:Bisbenzimidazole