The effect of chloramphenicol and cycloheximide on the activity of enzyme "markers" of mitochondrial substructures of the rat liver.
Chloramphenicol and cycloheximide exert in vivo inhibitory effects on the synthesis of mitochondrial enzymes. Choloramphenicol action results in a significant decrease of the activity of enzymes being the "markers" of all submitochondrial structures including that of the outer membrane. This suggests that chloramphenicol affects biosynthesis of of "assembly protein" on mitochondrial ribosomes and thus affects incorporation of proteins, wherever they are synthesized, into the structure of the mitochondrion as a functional entity. The effect of cycloheximide depends markedly on the concentration of the inhibitor and on the time of its administration. The differences in sensitivity of examined enzymes to the drug are probably related to their differential turnover.
D000818:Animals; D002701:Chloramphenicol / Q000494:pharmacology*; D003513:Cycloheximide / Q000494:pharmacology*; D004791:Enzyme Inhibitors; D005260:Female; D005649:Fumarate Hydratase / Q000378:metabolism; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000201:enzymology; D008995:Monoamine Oxidase / Q000378:metabolism; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D051381:Rats; D013385:Succinate Dehydrogenase / Q000378:metabolism
D004791:Enzyme Inhibitors; D002701:Chloramphenicol; D003513:Cycloheximide; D013385:Succinate Dehydrogenase; D008995:Monoamine Oxidase; D009251:NADPH-Ferrihemoprotein Reductase; D005649:Fumarate Hydratase