Determination of sulphated disaccharides from chondroitin sulphates by high-performance liquid chromatography.
A sensitive method for the determination of chondroitin 4- and 6-sulphate is presented. After chondroitinase digestion of the chondroitin sulphate preparations, the obtained disaccharides are separated on a weak anion-exchange resin in a high-performance liquid chromatography system. The method was used to study 4-sulphate to 6-sulphate ratios in chondroitin sulphates prepared from bovine nasal cartilage and human nucleus pulposus. The results show clearly that these two preparations contain considerable amounts of both isomers.
D000818:Animals; D002356:Cartilage / Q000032:analysis; D002417:Cattle; D002807:Chondroitin / Q000031:analogs & derivatives*; D002809:Chondroitin Sulfates / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004187:Disaccharides / Q000032:analysis*; D006801:Humans; D007403:Intervertebral Disc / Q000032:analysis; D007536:Isomerism; D009994:Osmolar Concentration; D012754:Sharks; D014907:Whales
D004187:Disaccharides; D002807:Chondroitin; D002809:Chondroitin Sulfates