A simple electrophoretic technique for the estimation of complement C3 conversion: specific application to the investigation of anaphylactoid response to I.V. agents.
A simple electrophoretic technique employing commercially available agarose films is described for the routine estimation of plasma complement C3 conversion. This technique has particular value in the investigation of anaphylactoid responses in patients following the administration of intravenous hypnotic drugs, plasma substitutes or radio-contrast media.
D000530:Alfaxalone Alfadolone Mixture / Q000008:administration & dosage; D000707:Anaphylaxis / Q000209:etiology*; D003176:Complement C3 / Q000276:immunology*; D003170:Complement Pathway, Alternative; D003171:Complement Pathway, Classical; D004492:Edetic Acid / Q000494:pharmacology; D004587:Electrophoresis, Agar Gel / Q000379:methods; D006801:Humans; D007275:Injections, Intravenous / Q000009:adverse effects; D010455:Peptides / Q000276:immunology; D015054:Zymosan / Q000494:pharmacology
D003176:Complement C3; D010455:Peptides; D000530:Alfaxalone Alfadolone Mixture; D015054:Zymosan; D004492:Edetic Acid