[Rickettsia conorii and prowazekii plaque study in a chick fibroblast cell culture].
The results of the study of plaques formed by R. conorii (strain M 1) and R. prowazeki (strain E and erythromycin-resistant strain E) in chick fibroblast cell culture are presented. In this study the tissue monolayer was inoculated with rickettsiae suspended in various media, and media of different composition were used in the nutrient cover and for cell cultivation. The maximum plaque formation was observed under the following conditions: the monolayer of chick fibroblasts (seeding density was not less than 375,000 cells per 1 sq. cm) was grown in medium 199 with 5-10% of fresh fetal or calf serum and inoculated with rickettsiae suspended in heart-brain infusion; the nutrient cover was prepared on the basis of Seakem agarose (USA) and contained medium 199 (without antibiotics) and 10% of fresh fetal or calf serum. In these conditions R. conorii formed plaques 2 mm in diameter, the first plaques being observed on day 6, and most of them on days 7-9; the both strains of R. prowazeki formed plaques 1 mm in diameter, the first plaques being observed on days 8-9, and most of them on days 10-13.
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D003470:Culture Media; D005347:Fibroblasts; D006462:Hemolytic Plaque Technique; D012281:Rickettsia / Q000276:immunology*; D012283:Rickettsia prowazekii / Q000276:immunology*; D013997:Time Factors
D003470:Culture Media
Izuchenie bliashek rikketsiĭ Konori i Provacheka v kul'ture kletok kurinykh fibroblastov.