Modification of a DNA cloning vehicle to give a high strand-separation melting temperature.
We have constructed a recombinant DNA cloning vector with a high strand-separation melting temperature. This feature is the result of an insertion of a short sequence of dG:dC base pairs. The new vector is useful for cloning DNA segments in anticipation of forming R-loops for the purpose of either transcriptional mapping or gene isolation.
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D004274:DNA, Recombinant; D004331:Drosophila melanogaster / Q000235:genetics; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000235:genetics; D013696:Temperature; D014158:Transcription, Genetic
D004274:DNA, Recombinant; D012313:RNA