Application of microtiter solid-phase radioimmunoassay to the determination of hepatitis B e antigen.
Microtiter solid-phase radioimmunoassay (micro-SPRIA) was applied to the detection of hepatitis B e antigen (HBeAg) in the serum. Antibody against HBeAg to coat the microtiter plate and for radiolabeling was obtained from the serum of asymptomatic carriers of hepatitis B surface antigen by an affinity column of partially purified HBeAg. The micro-SPRIA was sensitive and could be performed without prior concentration of the test serum. HBeAg was detected in 21 (35%) out of 60 serum samples positive for hepatitis B surface antigen, all of which were negative by the conventional immunodiffusion method even after they had been concentrated threefold.
D001665:Binding Sites; D001667:Binding, Competitive; D006510:Hepatitis B Antibodies; D006511:Hepatitis B Antigens; D006801:Humans; D005779:Immunodiffusion; D011863:Radioimmunoassay
D006510:Hepatitis B Antibodies; D006511:Hepatitis B Antigens