Evaluation of the Microcult-GC kit as a screening method for the detection of Neisseria gonorrhoeae.
The Microcult-GC Kit was compared to the standard modified Thayer-Martin plate method for the isolation and presumptive identification of Neisseria gonorrhoeae. Two hundred and thirty specimens representing urethral, endocervical, rectal, and pharyngeal sites were inoculated to both media. If only genital and rectal cultures were compared, the overall agreement was 98 percent, with a false positive rate of 1/145 (0.7 percent) and a false negative rate of 2/145 (1.4 percent). However, in the pharyngeal cultures, 16 of 32 gave false positives, thus emphasizing the manufacturer's instructions to limit the kit's use to genital and rectal cultures. The kit system gives rapid presumptive results, has a long shelf life at room temperature, and needs little laboratory equipment for proper use.
D001431:Bacteriological Techniques; D002584:Cervix Uteri / Q000382:microbiology; D005069:Evaluation Studies as Topic; D005260:Female; D006069:Gonorrhea / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D008297:Male; D009344:Neisseria gonorrhoeae / Q000302:isolation & purification; D010614:Pharynx / Q000382:microbiology; D011933:Reagent Kits, Diagnostic / Q000592:standards*; D012007:Rectum / Q000382:microbiology; D014521:Urethra / Q000382:microbiology
D011933:Reagent Kits, Diagnostic