Purification of alpha-fetoprotein from mouse amniotic fluid by gel-entrapped antibody filtration.
A one-step immunospecific affinity chromatographic method for purification of mouse alpha-fetoprotein (AFP) directly from amniotic fluid is described. The procedure employed a rabbit anti-AFP immunoglobulin-G entrapped in a polyacrylamide gel matrix prepared as a gel slurry. The presence of impurities could not be demonstrated in the eluted fraction and the recovery of AFP was approximately 26%. Sufficient quantities of AFP were readily purified for raising antibodies and for purity studies.
D000418:Albumins; D000653:Amniotic Fluid / Q000276:immunology*; D000818:Animals; D000906:Antibodies; D001665:Binding Sites; D002850:Chromatography, Gel; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006168:Guinea Pigs; D007106:Immune Sera / Q000494:pharmacology; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D051379:Mice; D011247:Pregnancy; D011817:Rabbits; D014168:Transferrin; D000509:alpha-Fetoproteins / Q000302:isolation & purification*
D000418:Albumins; D000906:Antibodies; D007106:Immune Sera; D014168:Transferrin; D000509:alpha-Fetoproteins