The nucleotide sequence of human tRNAGly (anticodon GCC).
The sequence of tRNAGCCGly from human placenta was determined by recently developed postlabeling techniques. The tRNA was digested completely with RNases T1 and A in the presence of alkaline phosphatase, the oligonucleotides were 3'-terminally (3H)-labeled, mapped on PEI-cellulose thin layers, isolated, and sequenced by methods based on base-specific cleavages. Overlaps were obtained by readout sequencing techniques on polyacrylamide gels and PEI-cellulose thin layers. The thin-layer readout technique was used also to locate and identify modified nucleotides. The primary structure was found to exhibit a large degree of homology (94.6%) with silkworm tRNAGCCGly but only 67.6% homology with human tRNACCCGly.
D000926:Anticodon; D001483:Base Sequence; D005260:Female; D005998:Glycine; D006801:Humans; D009843:Oligoribonucleotides / Q000032:analysis; D010920:Placenta / Q000032:analysis*; D011247:Pregnancy; D012343:RNA, Transfer / Q000302:isolation & purification; D006163:Ribonuclease T1; D012260:Ribonucleases
D000926:Anticodon; D009843:Oligoribonucleotides; D012343:RNA, Transfer; D012260:Ribonucleases; D006163:Ribonuclease T1; D005998:Glycine