Labeling of human platelets with [111In) 8-hydroxyquinoline.
We have evaluated the factors influencing the labeling of human platelets in the presence of autologous plasma. The labeling efficiency was found to be dependent on a) the time and temperature of incubation, b) the platelet concentration, c) the concentration of citrate ions (in ACD anticoagulant), and d) the concentration of 8-hydroxyquinoline in the suspending medium. Contrary to what was expected, unsaturated transferrin was found not to interfere with the transfer of In-111 from the [111In] 8-hydroxyquinoline complex to the platelets. Based on the findings of this study, a protocol was established by which human platelets can be labeled with In-111 in plasma with a labeling efficiency of 55.5 +/- 9.3 (mean +/- 1 s.d.) percent.
D000925:Anticoagulants / Q000378:metabolism; D001792:Blood Platelets / Q000378:metabolism*; D006358:Hot Temperature; D006801:Humans; D006912:Hydroxyquinolines; D007204:Indium; D007501:Iron / Q000097:blood; D007553:Isotope Labeling / Q000379:methods*; D015125:Oxyquinoline / Q000378:metabolism; D010949:Plasma / Q000378:metabolism; D011868:Radioisotopes; D013997:Time Factors; D014168:Transferrin / Q000378:metabolism
D000925:Anticoagulants; D006912:Hydroxyquinolines; D011868:Radioisotopes; D014168:Transferrin; D007204:Indium; D015125:Oxyquinoline; D007501:Iron