[Monolayer culture of adult mouse hepatic cells secreting albumin and alpha fetoprotein].
Adult mouse liver cells obtained by enzymatic dispersion were maintained in primary cultures for up to 4 weeks. They retained some of the typical morphological and ultrastructural characteristics of hepatocytes. After 2 days of culture, structures similar to bile canaliculi were found and after 6 days clusters of small proliferating cells were noticed in the gaps of the monolayer formed by large well-spread hepatocytes. Almost all 3-day cultures began to secrete alphafetoprotein for about 2 weeks, whereas albumin was secreted throughout the period of culture.
D000418:Albumins / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D008099:Liver / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D008722:Methods; D051379:Mice; D008815:Mice, Inbred Strains; D000509:alpha-Fetoproteins / Q000378:metabolism*
D000418:Albumins; D000509:alpha-Fetoproteins
Culture en monocouches de cellules hépatiques de Souris adulte secrétant l'albumine et l'alphafoetoprotéine.