Isolation and analysis of H-2 and Ia alloantigens from wild mouse strains.
Immunoprecipitation and SDS-PAGE analysis were used to examine H-2 and Ia antigens from mouse strains with wild-derived MHC haplotypes. Antisera raised against the wild-derived strains contained anti-H-2 and anti-Ia antibodies which precipitated antigen molecules readily distinguishable by a single assay procedure. The antibodies to wild strains were cross-reactive with standard laboratory haplotypes. Evidence supporting the similar genetic organization of wild and standard haplotypes was found, including isolation of separate H-2K and H-2D molecules from a wild-derived strain, and isolation of two separate Ia molecules from a wild-derived strain.
D000818:Animals; D000835:Animals, Wild; D000937:Antigen-Antibody Reactions; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D005802:Genes, MHC Class II; D006183:H-2 Antigens / Q000235:genetics*; D007519:Isoantigens / Q000235:genetics*; D051379:Mice / Q000235:genetics*; D008815:Mice, Inbred Strains; D010641:Phenotype; D013045:Species Specificity
D006183:H-2 Antigens; D007519:Isoantigens