[Immunofluorescent diagnosis of Entamoeba histolytica trophocoites in preserved stool specimens of patients (author's transl)].
A fixative and examination technique is described for identification of Entamoeba histolytica trophocoites in faeces with the aid of the indirect immunofluorescence technique. Magnaforms in bloody-dysenteric specimens and Minutaforms in spontaneous or saline purged specimens give equally good fluorescence. The specifity of the rabbit antiserum against axenically grown E. hist. is good: There is strong positive reaction only with trophocoites of E. hist. Weak crossreactions are encountered with trophocoites of E. coli, E. hartmanni and E. polecki. Negative reactions are encountered with trophocoites of Endolimax nana, Jodamoeba bāutschlii, and Dientamoeba fragilis. Amebic cysts, flagellates, leucocytes, epithelial cells and Blastocytis give negative reactions, too.
D000818:Animals; D003937:Diagnosis, Differential; D004404:Dysentery, Amebic / Q000175:diagnosis*; D004748:Entamoeba histolytica / Q000302:isolation & purification*; D005243:Feces / Q000469:parasitology*; D005404:Fixatives; D005455:Fluorescent Antibody Technique; D006801:Humans; D013045:Species Specificity; D013194:Staining and Labeling
D005404:Fixatives
Immunofluoreszenzoptischer Erregernachweis in fixierten Stuhlproben von Patienten mit intestinaler Amoebiasis.