Effect of triethylenetetramine dihydrochloride on the antibiotic susceptibility of Pseudomonas aeruginosa.
A chelating agent, triethylenetetramine dihydrochloride, interacted synergistically in vitro with both gentamicin and carbenicillin against a clinical isolate of Pseudomonas aeruginosa designated Ps 15. The minimal inhibitory concentrations of carbenicillin and gentamicin for Ps 15 in a 50% serum-Trypticase soy broth were 250 and 72.9 mug/ml, respectively. However, addition of triethylenetetramine dihydrochloride to the 50% serum-Trypticase soy broth reduced the minimal inhibitory concentration of both antibiotics approximately 10-fold. A comparison of the growth of Ps 15 in 50% serum-Trypticase soy broth containing either of the antibiotics showed that a rapid decrease in the percentage of survivors only occurred when the chelating agent was present.
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001790:Blood Physiological Phenomena; D002118:Calcium / Q000494:pharmacology; D002228:Carbenicillin / Q000494:pharmacology; D004357:Drug Synergism; D005029:Ethylenediamines / Q000494:pharmacology*; D005839:Gentamicins / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D014266:Trientine / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D005029:Ethylenediamines; D005839:Gentamicins; D002228:Carbenicillin; D014266:Trientine; D002118:Calcium