title: Effect of levulinic acid on pigment biosynthesis in Agmenellum quadruplicatum.
abstract: When levulinic acid was added to a growing culture of the cyanobacterium (blue-green alga) Agmenellum quadruplicatum PR-6, delta-aminoelevulinic acid accumulated in the medium and chlorophyll a synthesis and cell growth were inhibited, but there was a small amount of c-phycocyanin synthesis. The amount of delta-aminolevulinic acid produced in the treated culture did not fully account for the amount of pigment synthesized in the untreated control. Levulinic acid and either sodium nitrate or ammonium chloride were added to nitrogen-starved cultures of PR-6, and delta-aminolevulinic acid production and chlorophyll a and c-phycocyanin content were monitored. When ammonium chloride was added as a nitrogen source after nitrogen starvation, the cells recovered more rapidly than when sodium nitrate was added as a nitrogen source. In cultures recovering from nitrogen starvation, synthesis of c-phycocyanin occurred before synthesis of chlorophyll a.
mesh_terms: D000622:Aminolevulinic Acid / Q000378:metabolism; D000643:Ammonium Chloride / Q000494:pharmacology; D002734:Chlorophyll / Q000096:biosynthesis; D000458:Cyanobacteria / Q000187:drug effects* / Q000378:metabolism; D007982:Levulinic Acids / Q000494:pharmacology*; D009566:Nitrates / Q000494:pharmacology; D010798:Phycocyanin / Q000096:biosynthesis; D010860:Pigments, Biological / Q000096:biosynthesis*
chemical_list: D007982:Levulinic Acids; D009566:Nitrates; D010860:Pigments, Biological; D000643:Ammonium Chloride; D010798:Phycocyanin; D002734:Chlorophyll; D000622:Aminolevulinic Acid