An immunological determination of specific activities of enzymes: its use in quantification of cross reactivity between enzymes of different origins.
An immunological method is presented which enables the determination of the specific activity of a pure enzyme without its extensive purification. The method consists essentially in the specific fixation of immunologically related enzymes to an immunoadsorbent containing specific antibodies raised against the wild-type form of the enzyme. We applied this method to determine the specific activity of plasmid-coded beta-galactosidases and to quantify the extent of cross-reaction between these enzymes and Escherichia coli beta-galactosidase.
D000818:Animals; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000201:enzymology*; D005696:Galactosidases / Q000276:immunology*; D010957:Plasmids; D011817:Rabbits; D001616:beta-Galactosidase / Q000276:immunology*
D005696:Galactosidases; D001616:beta-Galactosidase