Albumin-coated Amberlite XAD-7 resin for hemoperfusion in acute liver failure. Part I: adsorption studies.
Platelets adhere to most foreign surfaces. As a result, polymers and albumin have been suggested as possible coatings to improve the blood compatibility of such surfaces. Amberlite XAD-7 has a high affinity for human serum albumin (HSA) and several protein-bound toxic metabolites. In the present study it is shown that HSA can be tightly bound to Amberlite XAD-7 without the use of chemical coupling agents. Optimal binding was achieved at pH 5.0 and the adsorption conforms to Langmuir's isotherm. Theoretical analysis of data and absence of a residual surface coating visible with scanning electron microscopy suggest a monolayer of albumin. The amount of HSA eluted from the resin under severe flow conditions was negligible (approximately 1%). Furthermore, in stirred batch studies with human plasma, the HSA coating did not decrease the adsorptive capacity of XAD-7 for bile acids and bilirubin.
D000180:Acrylic Resins; D000208:Acute Disease; D000327:Adsorption; D001665:Binding Sites; D002234:Carbodiimides / Q000494:pharmacology; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D005976:Glutaral / Q000494:pharmacology; D006464:Hemoperfusion; D006801:Humans; D008107:Liver Diseases / Q000628:therapy*; D011137:Polystyrenes; D012709:Serum Albumin; D013499:Surface Properties
D000180:Acrylic Resins; D002234:Carbodiimides; D011137:Polystyrenes; D012709:Serum Albumin; C015661:amberlite XAD 7; D005976:Glutaral