Improved preparation and structural elucidation of the tryptophan cleavage reagent 2-(2'-nitro-phenylsulfenyl)-3-methyl-3-bromoindolenine (BNPS-skatole).
Methods are described for a high yield preparation (overall yield greater than 90%) of analytically pure 2-(2'-nitrophenylsulfenyl)-3-methyl-3-bromoindolenine (BNPS-skatole). The chromatographic and spectroscopic data are given. The yield of tryptophan cleavage is considerably dependent on the degree of purity of the BNPS-skatole.
D055598:Chemical Phenomena; D002621:Chemistry; D006801:Humans; D007145:Immunoglobulin kappa-Chains; D007211:Indoles / Q000031:analogs & derivatives / Q000592:standards; D010446:Peptide Fragments; D011506:Proteins; D012862:Skatole / Q000031:analogs & derivatives / Q000592:standards; D013434:Sulfenic Acids / Q000592:standards; D014364:Tryptophan
D007145:Immunoglobulin kappa-Chains; D007211:Indoles; D010446:Peptide Fragments; D011506:Proteins; D013434:Sulfenic Acids; C005904:BNPS-skatole; D014364:Tryptophan; D012862:Skatole