A detergent-citric acid technique for isolating nuclear and cytoplasmic fractions containing undegraded RNA from cells of Xenopus laevis.
D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D002460:Cell Line; D002467:Cell Nucleus / Q000032:analysis*; D002951:Citrates; D003593:Cytoplasm / Q000032:analysis*; D011092:Polyethylene Glycols; D012313:RNA / Q000302:isolation & purification*; D012260:Ribonucleases / Q000037:antagonists & inhibitors; D014981:Xenopus
D002951:Citrates; D011092:Polyethylene Glycols; D012313:RNA; D012260:Ribonucleases