[An experimental study on Pseudomonas osteomyelitis with special reference to the production of experimental osteomyelitis in mice (author's transl)].
I) The author has successfully produced a model of experimental osteomyelitis caused by pseudomonas aeruginosa using the following procedure though such a demonstration has been said to be very difficult. After impregnation in a solution containing about 10(5) pseudomonas aeruginosa, 3 mm silk thread of No. 5 was dried under low-pressure atmosphere and then inserted into the metaphysis of right tibia of a mouse. This method can be produced experimental osteomyelitis in 100% of the animals. In the experimental osteomyelitis generated pathologically by this method, inoculated organisms do not transmigrate into blood, the kidney and the contralateral tibia. This may therefore be regarded as a local infection causing no death, making a long period of observation possible. In view of the X-ray and patho-histological findings, it is similar to human osteomyelitis. Furthermore, its host is a pure-bred mouse with constant elements making a league-scale experiment possible. II) This is an experimental model of osteomyelitis proved quite useful for the quantitative analysis of the effects of antibiotics, and would be a good method for evaluation of antibiotics to be developed in the future.
D000818:Animals; D001431:Bacteriological Techniques; D003982:Dibekacin / Q000627:therapeutic use; D005839:Gentamicins / Q000627:therapeutic use; D051379:Mice; D010019:Osteomyelitis / Q000209:etiology* / Q000382:microbiology / Q000473:pathology; D011552:Pseudomonas Infections / Q000209:etiology* / Q000382:microbiology / Q000473:pathology; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification; D012853:Sisomicin / Q000627:therapeutic use; D013977:Tibia / Q000473:pathology
D005839:Gentamicins; D003982:Dibekacin; D012853:Sisomicin