[Leucosis diagnosis in cattle using the Sudan black B staining method on granulocytes and monocytes].
In the peripheral blood of healthy cattle and cattle suffering from leucosis a positive reaction with Sudan black B was found in neutrophilic and eosinophilic granulocytes: in healthy cattle at an intensity from ++ to ++++, and in cattle suffering from leucosis it was somewhat slighter (++ to +++). This finding can, to a certain extent, help in the distinguishing of reactive lymphocytosis from the leucosis of cattle. Compared with granulocytes the reaction of monocytes is markedly weaker: in healthy cattle at an intensity from 0 to (++), and in diseased cattle from 0 to (+++). In the bone marrow there is a significantly weaker reaction to Sudan black B in the group of large cells (neutrophilic and eosinophilic promyelocytes and myelocytes); in the group of healthy and diseases cattle the reaction is weaker than in neutrophilic and eosinophilic granulocytes of the peripheral blood. The reaction obtained with Sudan black B for lipids can be used as an aid for the distinguishing of cells of the myeloid, monocytic, and lymphoid order of peripheral blood and bone marrow in cattle leucosis.
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000378:metabolism; D006098:Granulocytes / Q000378:metabolism; D006651:Histocytochemistry; D007938:Leukemia / Q000175:diagnosis / Q000378:metabolism / Q000662:veterinary*; D050356:Lipid Metabolism; D009000:Monocytes / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods*
Vyuzití barvicí metody sudanovou cerní B u granulocytû a monocytû skotu pro diagnostiku leukóz.