Purification and biochemical characterization of a virus-specific reverse transcriptase from human osteosarcoma tissue.
A RNA-dependent DNA polymerase (RTase) was purified from human osteosarcoma tissue by successive column chromatography of the microsomal fraction on DEAE-cellulose (DE-23 and DE-52) and phosphocellulose. The purified enzyme has a molecular weight of about 68,000, a pH optimum of 8.1, a Mg2+ optimum of 0.8 mM, Mn2+ optimum of 1.0 mM and a KCl optimum of 60 mM. The enzyme transcribes (rA)n . (dT)12, (rC)n . (dG)12-18 and (2-O-methyl C)n . (dG)18, but is unable to transcribe (dA)n . (dT)10. The enzyme has no catalytic activity in the presence of oligodeoxynucleotide initiators alone, indicating the absence of terminal deoxynucleotidyl transferase. The purified enzyme is able to transcribe the heteropolymeric regions of a 70S RNA from R(Mu)LV. The presented data support the presence of a RNA-dependent DNA polymerase in human osteosarcoma tissue with biochemical properties, resembling those of C-type RNA tumor viruses.
D000293:Adolescent; D005260:Female; D006801:Humans; D008970:Molecular Weight; D012516:Osteosarcoma / Q000201:enzymology* / Q000382:microbiology; D011119:Polynucleotides; D012194:RNA-Directed DNA Polymerase / Q000302:isolation & purification* / Q000378:metabolism; D012190:Retroviridae / Q000201:enzymology*; D013379:Substrate Specificity; D014158:Transcription, Genetic
D011119:Polynucleotides; D012194:RNA-Directed DNA Polymerase