[Interaction of epidermal growth factor with human fibroblasts in culture: an autoradiographic study at the ultrastructural level].
The potent mitogen epidermal growth factor (EGF) binds to specific receptors on human fibroblasts. In the present study we have used a quantitative EM autoradiographic approach to visualize the events involved in the binding process. When 125I-EGF is incubated at 4 degrees C for 120 min, labeled EGF primarily localizes to the plasma membrane of the fibroblast but when incubated at 37 degrees C for 120 min., over  2/3 of the labeled material is internalized by the cell. The internalized radioacitivity is primarily localized to lysomes. These studies demonstrate a temperature-dependent internalization of EGF following initial binding to specific plasma membrane receptors.
D002462:Cell Membrane / Q000378:metabolism*; D002478:Cells, Cultured; D004815:Epidermal Growth Factor / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D008247:Lysosomes / Q000378:metabolism; D008566:Membranes / Q000378:metabolism; D010455:Peptides / Q000378:metabolism*; D011955:Receptors, Drug / Q000378:metabolism*; D013696:Temperature
D010455:Peptides; D011955:Receptors, Drug; D004815:Epidermal Growth Factor
Interaction du facteur de croissance épidermique avec des fibroblastes humains en culture : une étude autoradiographique à l'échelon ultrastructural.