Phospholipid composition of methane-utilizing bacteria.
The phospholipids of Methylococcus capsulatus, Methylosinus trichosporium, La Paz, and OBT were examined in relation to their qualitative and quantitative composition. M. capsulatus exhibited a phospholipid composition consisting of phosphatidylethanolamine, phosphatidylglycerol, cardiolipin, and phosphatidyl-choline. The esterified fatty acids were predominantly C16:0 and C16:1. M. trichosporium, La Paz, and OBT exhibited an essentially identical phospholipid composition consisting of phosphatidylmonomethylethanolamine, phosphatidyl-dimethylethanolamine, phosphatidylcholine, and phosphatidylglycerol. Only trace amounts (less than 1%) of cardiolipin were found in these organisms. The major esterified fatty acid in these organisms was C18:1 (87 to 90%). The monounsaturated fatty acids from all four organisms consisted of both cis and trans isomers, each of which contained delta8, delta9, delta10, and delta11 double-bond positional isomers.
D002308:Cardiolipins / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D008055:Lipids / Q000032:analysis; D008697:Methane / Q000378:metabolism; D008772:Methylococcaceae / Q000032:analysis* / Q000378:metabolism; D010743:Phospholipids / Q000032:analysis*; D013045:Species Specificity
D002308:Cardiolipins; D005227:Fatty Acids; D008055:Lipids; D010743:Phospholipids; D008697:Methane