A comparative study on the uptake and subsequent decarboxylation of monoamine precursors in cerebral microvessels.
The endothelial cells and pericytes of brain microvessels (capillaries and small veins) are equipped with an enzymatic barrier, impeding the passage of circulating amino acids, such as amine precursors, into the brain. The properties of this mechanism was studied in brain slices and isolated microvessels from various species including man and also fetal material, following incubation in dihydroxyphenylalanine (DOPA), 5-hydroxytryptophan (5-HTP) and dihydroxyphenylserine (DOPS). A stereospecific, energy-dependent uptake leading to accumulation in the brain microvessel walls was found in all species studied; this process was found to exist already prenatally. The capacity of decarboxylation, the second step in the trapping mechanism at the blood-brain interphase, showed considerable species variation. The enzyme was present also in fetal brain microvessels. Inhibition experiments provided support for the presence of monoamine oxidase, but absence of catechol-O-methyl transferase, in the microvessel walls.
D006916:5-Hydroxytryptophan / Q000378:metabolism*; D000818:Animals; D001142:Aromatic-L-Amino-Acid Decarboxylases / Q000378:metabolism; D001545:Benserazide / Q000494:pharmacology; D001812:Blood-Brain Barrier; D001921:Brain / Q000098:blood supply* / Q000196:embryology / Q000378:metabolism*; D002415:Cats; D002417:Cattle; D006224:Cricetinae; D003653:Decarboxylation; D004285:Dogs; D015103:Droxidopa / Q000378:metabolism*; D005260:Female; D006168:Guinea Pigs; D000882:Haplorhini; D006801:Humans; D007980:Levodopa / Q000378:metabolism*; D008297:Male; D051379:Mice; D008833:Microcirculation / Q000378:metabolism; D008856:Microscopy, Fluorescence; D011817:Rabbits; D051381:Rats; D012694:Serine / Q000031:analogs & derivatives*; D013045:Species Specificity; D013552:Swine
D012694:Serine; D007980:Levodopa; D001545:Benserazide; D006916:5-Hydroxytryptophan; D001142:Aromatic-L-Amino-Acid Decarboxylases; D015103:Droxidopa