[Preparation of hybrid antibodies with mouse IgG and ferritin specifities for the immune electron microscopic detection of cell membrane antigens].
Use of hybrid antibodies with one specifity for mouse-IgG and the other one for ferritin is particularly suitable for immune electron microscopical detection of cell surface antigens. Preparation of antibodies of such kind is described, whereby the method introduced by HMMERLING et al. is varied within some steps of preparation. These variations are discussed here. Activity of produced hybrid antibodies is demonstrated by labeling the THY 1.1. antigen on the cell surface of the thymocytes of the mouse. The advantages of utilizing the hybrid antibodies in comparison with known immune electron microscopical techniques are an excellent location of the antigens, the possibility of using distinct particles for labeling, the application of a multiple labeling, and the fact that investigations by both the transmission and the scanning electron microscope can be carried out by means of the same preparation of hybrid antibodies.
D000818:Animals; D000906:Antibodies; D000954:Antigens, Surface / Q000032:analysis*; D002462:Cell Membrane / Q000648:ultrastructure*; D005293:Ferritins; D007118:Immunoassay; D005779:Immunodiffusion; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D051379:Mice; D008854:Microscopy, Electron; D013950:Thymus Gland / Q000032:analysis / Q000648:ultrastructure
D000906:Antibodies; D000954:Antigens, Surface; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D005293:Ferritins
Herstellung von Hybridantikörpern gegen Maus-IgG und gegen Ferritin zur immunelektronenmikroskopischen Darstellung von Zellmembranantigenen.