Histochemical localization of glycosidases in dog epididymis.
The histochemical localization of five glycosidases was studied in the epididymis of mature dogs. beta-Galactosidase showed a distinct to strong reaction in the epithelium of the ductuli efferentes and throughout the whole length of the ductus epididymidis. beta-N-Acetylglucosaminidase reactivity was weak in the initial segment, but increased significantly in the middle and terminal segment. The maximum beta-glucuronidase activity was found in the ductuli efferentes and in the initial segment. The alpha-mannosidase reaction was weak in all segments except the middle segment where a distinct activity was seen. With the method employed, no alpha-fucosidase activity could be detected. The physiological role of the glycosidases in the epididymis is discussed briefly.
D000118:Acetylglucosaminidase / Q000302:isolation & purification; D000818:Animals; D004285:Dogs; D004822:Epididymis / Q000201:enzymology*; D005966:Glucuronidase / Q000302:isolation & purification; D006026:Glycoside Hydrolases / Q000302:isolation & purification*; D006651:Histocytochemistry; D008297:Male; D008361:Mannosidases / Q000302:isolation & purification; D005644:alpha-L-Fucosidase / Q000302:isolation & purification; D001616:beta-Galactosidase / Q000302:isolation & purification
D006026:Glycoside Hydrolases; D008361:Mannosidases; D001616:beta-Galactosidase; D005966:Glucuronidase; D005644:alpha-L-Fucosidase; D000118:Acetylglucosaminidase