title: Mice whose B cells cannot produce the T15 idiotype also lack an antigen-specific helper T cell required for T15 expression.
abstract: The X-linked CBA/N defect in B cell function precludes an antibody response to phosphorylcholine (PC). Accordingly, (CBA/N X BALB/c)F1 male mice are unresponsive to PC and lack circulating immunoglobulin bearing the T15 idiotype characteristic of BALB/C anti-PC antibody. In contrast, (CBA/N X BALB/c)F1 female mice respond to PC and greater than 80% of the anti-PC antibody is T15+. No T-cell abnormalities are known to be associated with the CBA/N mutation. These experiments compared the ability of helper T cells from either (CBA/N X BALB/c)F1 male (T15-) or F1 female (T15+) mice to help F1 female B cells respond to PC and to influence the level of T15 expression. The results indicate that although F1 male T cells collaborated with F1 female B cells just as efficiently as F1 female T cells for the total anti-PC response, the percentage of T15 expression induced by F1 male T cells fell dramatically. The (CBA/N X BALB/c)F1 male thus appear to lack a helper T-cell subset required for dominant idiotype production. This helper T cell defect could be repaired by adding F1 female T cells primed to a second carrier to F1 male T cells and restimulating the cell mixture with PC coupled to the antigen used to prime the F1 male cells plus free second carrier. This result implies that conventional helper T cells derived from the F1 male donor can collaborate with a distinct helper T-cell subset from the F1 female donor which recognizes both carrier and idiotype to induce an anti-PC antibody response dominated by the T15 clonotype.
mesh_terms: D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000276:immunology*; D002352:Carrier Proteins / Q000276:immunology; D000939:Epitopes; D005260:Female; D008040:Genetic Linkage; D007130:Immunoglobulin Idiotypes; D008211:Lymphocyte Cooperation; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010767:Phosphorylcholine / Q000276:immunology; D011817:Rabbits; D013601:T-Lymphocytes / Q000276:immunology*; D014960:X Chromosome
chemical_list: D002352:Carrier Proteins; D000939:Epitopes; D007130:Immunoglobulin Idiotypes; D010767:Phosphorylcholine