[Enzyme-histochemical behaviour of the tanycyte ependyma in the III. ventricle of the rat with thiamin deficiency (author's transl)].
The influence of experimental thiamine deficiency in rats on enzyme-histochemical parameters was carried out in the diencephalon. The degree of thiamine deficiency was measured by the activity coefficient of transketolase in the red blood cells. The alpha-ETK of thiamine deficient animals was 1,45 +/- 0,36, of equal-fed animals 1,04 +/- 0.04 and of control animals 1,08 +/- 0,04. We got the following results: Lactatdehydrogenase, acid phosphatases and thiamine pyrophosphatase did not indicate any difference in enzyme activity between the groups of control, equal-fed and thiamine deficient rats. However, the enzyme activity of nonspecific lysosome-bound esterases in the tanycyte ependyma and in the investigated nuclear regions (nucleus supraopticus, ncl. paraventricularis, ncl. ventromedialis) of the thiamine deficient animals was significantly decreased in comparison to control and equal-fed animals. The possibility of a restriction of the hypophyseal-adrenal-cortex-system is discussed, since the results are similar to those obtained with bilaterally adrenalectomized rats.
D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D002552:Cerebral Ventricles / Q000201:enzymology*; D004027:Diencephalon / Q000201:enzymology*; D004805:Ependyma / Q000201:enzymology*; D004950:Esterases / Q000378:metabolism; D006651:Histocytochemistry; D006867:Hydrolases / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D010088:Oxidoreductases / Q000378:metabolism*; D051381:Rats; D013832:Thiamine Deficiency / Q000201:enzymology*; D013834:Thiamine Pyrophosphatase / Q000378:metabolism
D010088:Oxidoreductases; D007770:L-Lactate Dehydrogenase; D006867:Hydrolases; D004950:Esterases; D000135:Acid Phosphatase; D013834:Thiamine Pyrophosphatase
Das enzymhistochemische Verhalten des Tanycytenependyms im III. Ventrikel der Ratte bei Thiaminmangelernährung.