[Rule of antibody structure: the primary structure of a human monoclonal IgA1-immunoglobulin (myeloma protein Tro), II. Cleavage of the monomer IgA-molecule and the reduced and alkylated H- and L-chains by cyanogen bromide (author's transl)].
The monomer of myeloma protein Tro as well as the reduced and alkylated H- and L-chains were cleaved by cyanogen bromide. All cyanogen-bromide fragments were isolated and characterized by amino acid analyses, end-group and molecular weight determinations. The 4 smaller fragments of the 5 H-chain fragments were split with trypsin. The peptides were isolated and their primary structure was determined.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D003488:Cyanogen Bromide; D006801:Humans; D007070:Immunoglobulin A; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D046911:Macromolecular Substances; D009101:Multiple Myeloma / Q000276:immunology; D009194:Myeloma Proteins; D010446:Peptide Fragments / Q000032:analysis
D000596:Amino Acids; D007070:Immunoglobulin A; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D046911:Macromolecular Substances; D009194:Myeloma Proteins; D010446:Peptide Fragments; D003488:Cyanogen Bromide
Zur Strukturregel der Antikörper. Die Primärstruktur eines monoklonalen IgA1-Immunglobulins (Myelomprotein Tro), II. Spaltung des monomeren IgA sowie seiner H- und L-Kette mit Bromcyan.