TRH-like immunoreactivity in rat pancreas and eye, bovine and sheep pineals, and human placenta: non-identify with synthetic Pyroglu-His-Pro-NH2 (TRH).
Extracts from albino rat eyes and pancreas, bovine and sheep pineals and human placenta containing TRH-like immunoreactivity were chromatographed on silica-gel plates. Comparison of elution profiles for TRH-like immunoreactivity with that of TRH revealed the presence of substances other than TRH in these samples. Chromatography of TRH-like immunoreactivity obtained from Rana pipiens skin eluted in two solvent systems produced elution profiles identical with that of synthetic Pyroglu-His-Pro-NH2 consistent with reports that frog skin contains large quantities of TRH. Implications of these findings are discussed.
D000818:Animals; D001001:Anura; D002417:Cattle; D002850:Chromatography, Gel; D002855:Chromatography, Thin Layer; D003429:Cross Reactions; D005123:Eye / Q000032:analysis*; D005260:Female; D006728:Hormones / Q000032:analysis / Q000276:immunology*; D006801:Humans; D010179:Pancreas / Q000032:analysis*; D010870:Pineal Gland / Q000032:analysis*; D010920:Placenta / Q000032:analysis*; D011247:Pregnancy; D011863:Radioimmunoassay / Q000379:methods; D011894:Rana pipiens; D051381:Rats; D012756:Sheep; D012867:Skin / Q000032:analysis; D013973:Thyrotropin-Releasing Hormone / Q000032:analysis* / Q000276:immunology
D006728:Hormones; D013973:Thyrotropin-Releasing Hormone