[3H]serotonin release: an improved method to measure mast cell degranulation.
A method based on the release of tritium-labelled serotonin by activated mast cells in rodents is described. Mast cells incorporate labelled serotonin selectively and release the label after activation by non-specific stimulators (compound 48/80, polymyxin B sulphate, ATP, bovine chymotrypsin and L-alpha-lysophosphatidylcholine) or anaphylactic antibody and the corresponding antigen. These two types of activation were investigated in comparison with the toluidine blue microscopic rat mast cell degranulation test, and a methodological study of the release of [3H]serotonin is described. The measurement of labelled serotonin release provides a simple and quick assay of mast cell degranulation compared to the time required for the classical rat mast cell degranulation technique and achieves a greater sensitivity.
D000818:Animals; D000937:Antigen-Antibody Reactions; D001202:Ascitic Fluid / Q000276:immunology; D003374:Coumarins / Q000494:pharmacology; D004205:Cromolyn Sodium / Q000494:pharmacology; D003594:Cytoplasmic Granules / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D008407:Mast Cells / Q000276:immunology*; D051379:Mice; D008808:Mice, Inbred CBA; D010208:Papaverine / Q000494:pharmacology; D051381:Rats; D012701:Serotonin / Q000378:metabolism*; D014316:Tritium; D003189:p-Methoxy-N-methylphenethylamine / Q000494:pharmacology
D003374:Coumarins; D014316:Tritium; D012701:Serotonin; D003189:p-Methoxy-N-methylphenethylamine; D010208:Papaverine; D004205:Cromolyn Sodium