Differentiation of antibodies against Brucella abortus and Yersinia enterocolitica by enzyme-linked immunosorbent assay.
The enzyme-linked immunosorbent assay (ELISA) was found to be from ten to hundred-fold more sensitive than commonly used tube agglutination text. The ELISA using both direct and indirect inhibition assays revealed antigenic differences between Brucella abortus and Yersinia enterocolitica O-group V (serotype 9), which are undetected by commonly used serological routine tests. The results point to the possibility that the ELISA assay can be used as a serological method for differentiation between antibodies against different Brucella species and Y. enterocolitica O-group V.
D000372:Agglutination Tests; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D000918:Antibody Specificity; D002003:Brucella abortus / Q000276:immunology*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D007124:Immunoenzyme Techniques; D008070:Lipopolysaccharides / Q000276:immunology; D011817:Rabbits; D015007:Yersinia / Q000276:immunology*
D000907:Antibodies, Bacterial; D000939:Epitopes; D008070:Lipopolysaccharides