[Two-layer rocket-electrophoresis on cellulose-acetate membranes].
Two-floor rocket-electrophoresis on gelatinated acetate cellulose membrane "Cellogel" has been developed. The method is based on electroimmunodiffusion detection of the antigen on the acetate-cellulose membranes, containing monospecific antiserum of the test-system. The procedure is followed by the detection of precipitation bands ("rockets") by staining, if the reaction is conducted in the visible zone or by the further treatment of the acetate-cellulose strips, containing invisible precipitates with antiglobulin antibodies, the complement or their combination. An increase in the method sensitivity up to 30-60 ng/ml in the visible zone of the reaction is achieved by simultaneous reduction in the antibody concentration and the growth of the absolute quantity of the antigen, subjected to electrophoresis, up to 50-100 microliters. The method has been applied to human alpha-fetoprotein.
D004588:Electrophoresis, Cellulose Acetate; D006801:Humans; D007122:Immunoelectrophoresis / Q000379:methods*; D008832:Microchemistry; D000509:alpha-Fetoproteins / Q000032:analysis*
D000509:alpha-Fetoproteins
Dvukh"iarusnyĭ roket-elektroforez na atsetiltselliuloznykh plenkakh.