Trypsin-orcein banding in plant chromosomes.
A convenient and quick method using trypsin-orcein for banding plant chromosomes (O-banding) is suggested. The technique is directly applicable to meristematic tissues (e.g. root tips) and involves the treatment of root tips with 1-2% solution of trypsin either in buffer or in 0.5 N HCl for 5-10 minutes at 37 C or for 30-60 minutes near 0 C followed by staining with 1.5% acetic orcein: 1 N HCl (19:1). Dark staining bands are reproducible and species specific. These bands possibly represent specific DNA-protein-dye interaction.
D002871:Chromosome Banding / Q000379:methods*; D010944:Plants / Q000648:ultrastructure*; D013194:Staining and Labeling; D014357:Trypsin / Q000494:pharmacology
D014357:Trypsin