Prolongation of islet allograft survival following in vitro culture (24 degrees C) and a single injection of ALS.
Isolated rat islets remain morphologically and functionally intact during a 7-day period of in vitro culture at 24 degrees C. In vitro culture of islets at 24 degrees C for 7 days prior to transplantation, in conjunction with a single injection of antiserum to lymphocytes into the diabetic recipient, results in islet allograft survival of 100 days when the islets are transplanted across a major histocompatibility barrier.
D000818:Animals; D000961:Antilymphocyte Serum; D046508:Culture Techniques; D003921:Diabetes Mellitus, Experimental / Q000628:therapy; D006085:Graft Survival; D006649:Histocompatibility Antigens; D016381:Islets of Langerhans Transplantation; D008297:Male; D051381:Rats; D013997:Time Factors; D014184:Transplantation, Homologous
D000961:Antilymphocyte Serum; D006649:Histocompatibility Antigens