[A method for testing susceptibility of Haemophilus influenzae to co-trimoxazole (author's transl)].
Various methods for determining the activity of co-trimoxazole against haemophilus influenzae have been compared. The results were cortically influenced by antagonistic substances in the medium. The content of p-aminobenzoeacid and thymidine in the medium should be as low as possible. The antagonistic influences are compensated by lysed horseblood, which contains thymidine-phosphorylase. Haeminchloride and nicotinamide adenine dinucleotide (NAD) are better growth-factors than other poorly defined preparations (Supplement C, Fil-des-Enrichment, Iso-VitaleX). For the determination of the minimal inhibition concentration (MIC) we propose the microtiter technique. Likewise a modified agar diffusion test can be recommended for screening purposes. Of the 143 haemophilus influenzae strains tested against co-trimoxazole 134 (94%) were sensitive.
D010129:4-Aminobenzoic Acid / Q000032:analysis; D003470:Culture Media / Q000032:analysis; D004352:Drug Resistance, Microbial; D006193:Haemophilus influenzae / Q000187:drug effects*; D005779:Immunodiffusion; D008826:Microbial Sensitivity Tests; D013420:Sulfamethoxazole / Q000494:pharmacology*; D013936:Thymidine / Q000032:analysis; D014295:Trimethoprim / Q000494:pharmacology*
D003470:Culture Media; D014295:Trimethoprim; D013420:Sulfamethoxazole; D010129:4-Aminobenzoic Acid; D013936:Thymidine
Resistenzbestimmung von Haemophilus influenzae gegen Co-Trimoxazol.