Conversion of 2,4,6-trinitrophenol to a mutagen by Pseudomonas aeruginosa.
A strain of Pseudomonas aeruginosa reduced 2,4,6-trinitrophenol (picric acid) to 2-amino-4,6-dinitrophenol (picramic acid) under anaerobic conditions. Mutagenic assays of picric acid and picramic acid were carried out with histidine-requiring strains of Salmonella typhimurium. Picric acid (10 micrograms per plate) demonstrated mutagenicity (both frame shift and base substitution-gype mutations) only after activation with a rat liver homogenate preparation. Picramic acid (1 microgram per plate) induced both base pair substitution and frame shift-tupe mutations without activation by the rat liver preparation.
D000693:Anaerobiosis; D001673:Biodegradation, Environmental; D004140:Dinitrophenols / Q000378:metabolism / Q000494:pharmacology; D009153:Mutagens; D010851:Picrates / Q000378:metabolism* / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D012486:Salmonella typhimurium / Q000187:drug effects; D012722:Sewage; D012988:Soil Microbiology; D014871:Water Microbiology
D004140:Dinitrophenols; D009153:Mutagens; D010851:Picrates; D012722:Sewage