Isolation and purification of rat mammary tumor peroxidase.
7,12-Dimethylbenz(a)anthracene-induced rat mammary tumors often contain high levels of the enzyme perioxidase, a putative marker of estrogen dependence. This enzyme can be effectively extracted with 0.5 M CaCl2, giving rise to a soluble peroxidase with a molecular weight of about 50,000 as determined by gel filtration. This is the same size as the estrogen-induced peroxidase of rat uterus but smaller than other mammalian peroxidases. Further purification of the rat mammary tumor peroxidase by concanavalin A-Sepharose chromatography and hydrophobic interaction chromatography on phenyl Sepharose provides a 640-fold purification of the enzyme.
D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D002122:Calcium Chloride; D002846:Chromatography, Affinity; D005260:Female; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced / Q000201:enzymology* / Q000378:metabolism; D009376:Neoplasms, Hormone-Dependent / Q000139:chemically induced / Q000201:enzymology* / Q000378:metabolism; D010544:Peroxidases / Q000302:isolation & purification*; D051381:Rats; D011960:Receptors, Estrogen; D012995:Solubility
D011960:Receptors, Estrogen; D015127:9,10-Dimethyl-1,2-benzanthracene; D010544:Peroxidases; D002122:Calcium Chloride