Comparative biological and antitoxoplasmic effects of particulate and water-soluble polysaccharides, in vitro.
Mouse peritoneal macrophages, but not HeLa cells, presented many characteristics of activated cells following treatment in culture with LTN or PSK. Indeed, they demonstrated increase in size, spread, endocytic and antiparasitic activities. These data lead favorably to consider that among the five polysaccharides tested under the present experimental model LTN and PSK are the most potent activators of resident mouse peritoneal macrophages in vitro. The mechanism of macrophage activation by LTN and PSK should be different from that of Di Luzio glucans and SPG since activated macrophages can be induced by the latter two compounds in experimental animals but not in vitro in cell cultures.
D000818:Animals; D002448:Cell Adhesion; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D004705:Endocytosis / Q000187:drug effects; D005260:Female; D005936:Glucans / Q000494:pharmacology; D006367:HeLa Cells; D007912:Lentinan / Q000494:pharmacology; D008264:Macrophages / Q000502:physiology; D051379:Mice; D009068:Movement / Q000187:drug effects; D010587:Phagocytosis / Q000187:drug effects; D010873:Pinocytosis / Q000187:drug effects; D011135:Polysaccharides, Bacterial / Q000494:pharmacology*; D012566:Sizofiran / Q000494:pharmacology; D012995:Solubility; D014122:Toxoplasma / Q000187:drug effects*
D005936:Glucans; D011135:Polysaccharides, Bacterial; D007912:Lentinan; D012566:Sizofiran