Abnormalities in clonable B lymphocytes and myeloid progenitors in autoimmune NZB mice.
Cloning procedures were used to study B lymphocytes and progenitors of granulocytes and macrophages in NZB mice. Numbers of B cells that were detected in sheep erythrocyte-containing semisolid cultures were only slightly elevated in NZB tissues, and these were normally sensitive to inhibition by anti-mu or anti-delta antibodies or prostaglandin E. However, NZB mice rapidly developed large numbers of B cells that could be cloned in the presence of lipopolysaccharide, and these included unusual anti-mu resistant cells. Numbers of myeloid precursors in NZB bone marrow that were responsive to colony-stimulating activity in L-cell conditioned medium or endotoxin serum were at least normal, but at all ages granulocyte-macrophage precursors were poor responders in cultures stimulated by WEHI-3 cell conditioned medium. Almost no colonies were elicited in NZB cultures with a colony-stimulating activity moiety from WEHI-3 cells. Prostaglandin sensitivity of myeloid precursors from NZB and CBA mice was also different. Codominant genetic control of these abnormalities was suggested by their partial expression in F1 hybrid NZB X CBA and NZB X NZW mice. NZB mice expressed an unexpected IgD allotype allele.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000008:administration & dosage; D001323:Autoantibodies / Q000235:genetics; D001402:B-Lymphocytes / Q000276:immunology*; D002999:Clone Cells / Q000276:immunology; D005260:Female; D005802:Genes, MHC Class II; D006098:Granulocytes / Q000276:immunology; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D007072:Immunoglobulin D; D007148:Immunoglobulin mu-Chains / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D008297:Male; D051379:Mice; D008814:Mice, Inbred NZB; D011459:Prostaglandins E, Synthetic / Q000494:pharmacology
D000888:Antibodies, Anti-Idiotypic; D001323:Autoantibodies; D007072:Immunoglobulin D; D007148:Immunoglobulin mu-Chains; D011459:Prostaglandins E, Synthetic