Genetic regulation of tissue-specific expression of amylase structural genes in Drosophila melanogaster.
Laboratory strains of Drosophila melanogaster were screened for spatial variations in adult midgut alpha-amylase (1,4-alpha-D-glucan glucanohydrolase, EC 3.2.1.1) expression. No strain-specific differences were found anteriorly, but three patterns of activity were discerned in the posterior midgut: A, activity throughout most of the region; B, activity in the anterior part of the region; and C, little or no activity. Alleles of a control gene, map, are responsible for this tissue-specific regulation of activity; e.g., mapA homozygotes produce the A pattern and mapC homozygotes the C pattern. The map locus was placed at 2--80 +/- on the genetic map of chromosome 2R, about two crossover units distal to the Amy structural gene region for alpha-amylase. Electrophoretic studies showed that mapA is trans acting in mapA/mapC flies, allowing expression of amylase isozymes coded for by genes on the opposite chromosome. The map gene behaves as a temporal gene that is clearly separable from the tightly linked, duplicated Amy structural genes.
D000681:Amylases / Q000235:genetics*; D000818:Animals; D002874:Chromosome Mapping; D004331:Drosophila melanogaster / Q000235:genetics*; D005796:Genes; D005809:Genes, Regulator; D008040:Genetic Linkage; D007422:Intestines / Q000201:enzymology; D007527:Isoenzymes / Q000235:genetics* / Q000378:metabolism; D011995:Recombination, Genetic; D014018:Tissue Distribution; D000516:alpha-Amylases / Q000235:genetics* / Q000378:metabolism
D007527:Isoenzymes; D000681:Amylases; D000516:alpha-Amylases