A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines.
We have isolated a subclone of the mouse myeloma cell line P3-X63-Ag8 that does not express immunoglobulin heavy or light chains. This clone X63-Ag8.653 can be used for efficient fusion with antibody-forming cells to obtain hybrid cell lines producing pure monoclonal antibodies. Screening of hybrid cell lines for specificity and immunoglobulin classes was done with a modified enzyme-linked immunosorbent assay.
D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology*; D002459:Cell Fusion; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D004797:Enzyme-Linked Immunosorbent Assay; D006822:Hybrid Cells / Q000276:immunology*; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D051379:Mice; D008810:Mice, Inbred C57BL; D009101:Multiple Myeloma / Q000276:immunology*; D011817:Rabbits
D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains