Preparation of protoplasts and whole cell ghosts from Mycobacterium smegmatis.
Cell wall-deficient forms of Mycobacterium smegmatis were produced in growth medium containing D-cycloserine and horse serum. These cells were transformed into protoplasts with EDTA and lysozyme. Subsequent lysis by nucleases followed by osmotic shock produced membrane vesicles (whole cell ghosts).
D000818:Animals; D001431:Bacteriological Techniques; D001769:Blood; D002462:Cell Membrane; D003523:Cycloserine / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D006736:Horses; D009113:Muramidase / Q000494:pharmacology; D009161:Mycobacterium / Q000187:drug effects / Q000648:ultrastructure*; D010406:Penicillins / Q000494:pharmacology; D011523:Protoplasts
D010406:Penicillins; D003523:Cycloserine; D004492:Edetic Acid; D009113:Muramidase