[Radioenzymatic micromethod for the determination of UDP-glucuronic acid (author's transl)].
A new senitive and specific micromethod for determination of UDP-glucuronic acid is described Extracts from 2.5 mg of liver are incubated with twice washed guinea pig microsomes (as a source of UDP-glucuronyl transferase) and [14C]p-nitrophenol. The content of UDP-glucuronic acid is calculated from the [14C]p-nitrophenyl glucuronide/[14C]p-nitrophenol radioactivity ratio and the known amount of introduced [14C]p-nitrophenol. These values are corrected for losses of UDP-glucuronic acid by a calibration experiment, containing in addition to the aforementioned constituents, a known amount of UDP-glucuronic acid. The mean concentration of UDP-glucuronic acid in mice liver was found to be 171 mumol/kg wet weight.
D000818:Animals; D002250:Carbon Radioisotopes; D014453:Glucuronosyltransferase / Q000378:metabolism; D006168:Guinea Pigs; D007553:Isotope Labeling / Q000379:methods; D007700:Kinetics; D008099:Liver / Q000032:analysis; D051379:Mice; D008861:Microsomes / Q000201:enzymology; D009596:Nitrophenols; D014535:Uridine Diphosphate Glucuronic Acid / Q000032:analysis*; D014539:Uridine Diphosphate Sugars / Q000032:analysis*
D002250:Carbon Radioisotopes; D009596:Nitrophenols; D014539:Uridine Diphosphate Sugars; D014535:Uridine Diphosphate Glucuronic Acid; D014453:Glucuronosyltransferase
Eine radioenzymatische Mikromethode zur Bestimmung der UDP-Glucuronsäure.