Physicochemical properties of pyocin F1.
The physiochemical properties of pyocin F1 were studied. Pyocin F1 consists of flexuous rod-like particles homogenous in size. Each particle was composed of rod and fiber parts. The rod part was 105.5 +/- 9.5 nm long and 10.0 +/- 1.4 nm wide, and showed regular striations amounting to 23 layers. The fiber part was composed of several filaments; the length of the longest filament was 43.0 +/- 12.0 nm. The amino acid composition, the partial specific volume (0.720 ml/g), the sedimentation coefficient (S020,W = 35.1S), and the translational diffusion constant (0.94 +/- 0.01 x 10(-7) cm2/s) were determined. The particle weight was calculated to be 3.23 x 10(6) daltons.
D000596:Amino Acids / Q000032:analysis; D001430:Bacteriocins / Q000302:isolation & purification; D008854:Microscopy, Electron; D008970:Molecular Weight; D011487:Protein Conformation; D011550:Pseudomonas aeruginosa / Q000032:analysis*; D011709:Pyocins / Q000302:isolation & purification
D000596:Amino Acids; D001430:Bacteriocins; D011709:Pyocins