Use of 2-aminoacetophenone production in identification of Pseudomonas aeruginosa.
A grapelike odor is often of diagnostic importance in detecting the growth of Pseudomonas aeruginosa in culture and in burn wounds. The compound responsible for the odor has been identified as 2-aminoacetophenone by mass spectroscopy. Although the grape odor is sometimes difficult to detect in culture media, gas chromatographic, fluorometric, and colorimetric methods can be utilized to assay 2-aminoacetophenone production in a variety of media. Its synthesis occurs relatively early in the growth cycle. It has proved easy and convenient to detect 2-aminoacetophenone excretion by P. aeruginosa after 24 h of incubation on blood agar plates employing a fluorometric assay of ether extracts of the agar medium.
D000098:Acetophenones / Q000378:metabolism*; D002056:Burns / Q000382:microbiology*; D003470:Culture Media; D005470:Fluorometry; D006801:Humans; D009812:Odorants; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification* / Q000378:metabolism; D011799:Quinazolines / Q000378:metabolism; D014364:Tryptophan / Q000378:metabolism
D000098:Acetophenones; D003470:Culture Media; D011799:Quinazolines; D014364:Tryptophan