Rocket immunoelectrophoresis in the presence of denaturing agents.
Modifications of the Laurel rocket technique for assaying antigen antibody reactions are described. These procedures allow insoluble proteins to be dissolved in a variety of denaturing solvents (e.g., SDS and urea) and subjected to electroimmunoassay without loss of sensitivity or specificity. Methods are also presented for obtaining rockets using gel slices from polyacrylamide gel electrophoresis either in the presence of urea or SDS. Results obtained using keratins, the insoluble proteins of epidermis, hair and nail are summarized.
D000818:Animals; D000906:Antibodies; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007633:Keratins / Q000276:immunology; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000276:immunology; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology*; D014508:Urea / Q000494:pharmacology*
D000906:Antibodies; D012710:Serum Albumin, Bovine; D012967:Sodium Dodecyl Sulfate; D007633:Keratins; D014508:Urea