Binding of antigen-enzyme complex by antigen-binding cells as an approach for immunogdiagnosis.
An immunoenzyme method capable of detecting specific antigen-binding cells is developed. The method is based on the chemical coupling of the antigen to beta-galactosidase and binding of the conjugate to the corresponding antigen-binding cells. Human hydatid fluid was used as an antigen model, and cells derived from rabbits immunized with human hydatid fluid showed specific binding to human hydatid fluid-beta-galactosidase conjugate. The method is specific and reproducible and could be used as an immunodiagnostic test for various diseases in which little or no antibody is produced.
D000818:Animals; D000941:Antigens; D001665:Binding Sites; D005260:Female; D007124:Immunoenzyme Techniques; D007962:Leukocytes / Q000276:immunology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D013154:Spleen / Q000166:cytology / Q000276:immunology; D001616:beta-Galactosidase / Q000276:immunology
D000941:Antigens; D001616:beta-Galactosidase