[Immunologic study of the cellular components of bacillus pyocyaneus. III. Immunochemical analysis, toxicity and protective properties of water-soluble antigenic complexes].
Aqueous extracts of two Ps. aeruginosa strains killed with acetone were subjected to fractionation by preparative ultracentrifugation and gel-chromatography. Toxic activity of the extract was found to be connected with the high-molecular, possibly glycoprotein components reacting with the corresponding antiserum in the immunoprecipitation test, and protecting 30--40% of rats against the generalized infection with Pseudomonas aeruginosa. This protective activity is apparently connected with the protein components (molecular weight--20000--60000 dalton), nontoxic for mice, not reacting with the corresponding antiserum in the immunoprecipitation test, and protecting 60 to 80% of rats against Ps. aeruginosa infecsion. Thus, as a result of preparative ultracentrifugation and gel-chromatography it was postible to divide the toxic and the nontoxic protective components of Ps. aeruginosa.
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis*; D001426:Bacterial Proteins / Q000032:analysis; D001427:Bacterial Toxins / Q000032:analysis* / Q000633:toxicity; D002458:Cell Fractionation; D055598:Chemical Phenomena; D002621:Chemistry; D002850:Chromatography, Gel; D051379:Mice; D008970:Molecular Weight; D011234:Precipitins / Q000032:analysis; D011550:Pseudomonas aeruginosa / Q000032:analysis / Q000276:immunology*; D051381:Rats; D014461:Ultracentrifugation
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D011234:Precipitins
Immunologicheskoe izuchenie kletochnykh komponentov sinegnoĭnoĭ palochki. Soobshchenie III. Immunokhimicheskiĭ analiz, toksichnost' i protektivnye svoĭstva vodorastvorimykh antigennykh kompleksov.