[Two-step purification of estradiol calf uterine cytosol receptor].
The use of two affinity chromatography methods: heparin-Ultrogel and estradiol 7 alpha-(CH2)3-poly (alanine-lysine)-agarose, allows us to purify the calf uterine estradiol cytosol receptor with a 20% recovery. Electrophoresis under denaturing conditions allows us to estimate its molecular weight as 70 000 dalton.
D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity; D003600:Cytosol / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D006493:Heparin; D008970:Molecular Weight; D011960:Receptors, Estrogen / Q000302:isolation & purification* / Q000378:metabolism; D014599:Uterus / Q000378:metabolism*
D011960:Receptors, Estrogen; D004958:Estradiol; D006493:Heparin
Purification en deux étapes du récepteur cytosoluble de l'oestradiol d'utérus de veau.