title: Analysis of HGPRT- CRM+ human lymphoblast mutants.
abstract: Three 6-thioguanine-resistant mutants of the human diploid lymphoblast line MGL-8 were studied. The inactivation by heat of both HGPRT activity and antigenicity of the HGPRT immunologically cross-reacting material of the A30 mutant cells were not protected by PRPP, indicating that the HGPRT in A30 cells has an altered PRPP binding site, leading to lack of stabilization and rapid degradation of the enzyme. Two dimensional separations of the immunoprecipitates from extracts of the parental and mutant cell lines showed that the A35 mutant CRM has a more acidic isoelectric pH, while the A30 CRM has a more basic isoelectric pH and that the A30 protein has a faster rate of degradation than the wild-type HGPRT. The A30 CRM also has a smaller molecular size than the wild-type enzyme.
mesh_terms: D002460:Cell Line; D003429:Cross Reactions; D000939:Epitopes; D006358:Hot Temperature; D006801:Humans; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics* / Q000276:immunology; D008214:Lymphocytes; D009154:Mutation; D010443:Peptide Chain Termination, Translational; D011110:Polymorphism, Genetic
chemical_list: D000939:Epitopes; D007041:Hypoxanthine Phosphoribosyltransferase