Measurement of antibodies to tubulin by radioimmunoassay.
A solid-phase double antibody radioimmunoassay capable of measuring antibody to tubulin, the principal component of microtubules, is described. This assay is simple, combining sensitivity with specificity and also allowing determination of antibody subclasses.
D000818:Animals; D000906:Antibodies; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D002417:Cattle; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000276:immunology; D007142:Immunoglobulin gamma-Chains / Q000276:immunology; D007148:Immunoglobulin mu-Chains / Q000276:immunology; D011863:Radioimmunoassay; D012756:Sheep; D014404:Tubulin / Q000276:immunology* / Q000302:isolation & purification
D000906:Antibodies; D007141:Immunoglobulin Fc Fragments; D007142:Immunoglobulin gamma-Chains; D007148:Immunoglobulin mu-Chains; D014404:Tubulin