Effects of tetracyclines on aldosterone- and insulin-mediated Na+ transport in the toad urinary bladder.
The effect of oxytetracycline and demethylchlortetracycline on aldosterone- and insulin-mediated Na+ transport (short-circuit current) were examined in toad urinary bladders mounted in modified Ussing chambers. Oxytetracycline had little or no effect on either basal or aldosterone-mediated Na+ transport. In contrast, demethylchlortetracycline markedly inhibited both basal and aldosterone-mediated Na+ transport. Furthermore, demethylchlortetracycline inhibited the aldosterone response significantly out of proportion to its effects on basal Na+ transport. Neither of the drugs had an effect on insulin-mediated Na+ transport. Consequently, the natriuresis observed in certain patients treated with demethylchlortetracyline may be related to drug-induced renal resistance to the effects of aldosterone.
D000450:Aldosterone / Q000494:pharmacology*; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002024:Bufo marinus; D003707:Demeclocycline / Q000494:pharmacology*; D005260:Female; D007328:Insulin / Q000494:pharmacology*; D010118:Oxytetracycline / Q000494:pharmacology*; D012964:Sodium / Q000378:metabolism*; D001743:Urinary Bladder / Q000187:drug effects / Q000378:metabolism*
D007328:Insulin; D000450:Aldosterone; D003707:Demeclocycline; D012964:Sodium; D010118:Oxytetracycline