Effects of donor age on neoplastic transformation of adult mouse bladder epithelium in vitro.
Neoplastic transformation of C57BL/lcrf-a' mouse bladder epithelium was induced in long-term primary cultures by a single 24-hour treatment with 7,12-dimethylbenz[a]anthracene on day 2 of culture. Transformed foci appeared earlier (40--60 days) and at a higher frequency (28%) in cultures from old donors (28--30 mo) compared with 100 days and 0.9% in cultures from young adult donors (5--7 mo). After transplantation into syngeneic mice, transformed cells produced carcinomas. Spontaneous epithelial transformation occurred in dimethyl sulfoxide-treated old cultures after the same interval (40--60 days) as in the carcinogen-treated cultures but at a lower frequency (5.3%). Spontaneous epithelial transformation did not occur in cultures from young donors.
D015127:9,10-Dimethyl-1,2-benzanthracene; D000367:Age Factors; D000375:Aging; D000818:Animals; D002471:Cell Transformation, Neoplastic; D046508:Culture Techniques; D004848:Epithelium / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000209:etiology; D014185:Transplantation, Isogeneic; D001749:Urinary Bladder Neoplasms / Q000209:etiology* / Q000473:pathology
D015127:9,10-Dimethyl-1,2-benzanthracene