The use of 60Co-EDTA as an extracellular marker in frog skin.
60Co-EDTA was tested as an extracellular marker in isolated epithelia of frog skin. It was found to be non-toxic for frog skin and 0.1 mM EDTA proved to be enough to saturate any adsorption sites. Comparing with [14C]inulin, 60Co-EDTA marks a slightly greater extracellular space and this volume is constant between 15 min and 2 h. Furthermore it is reproducible and its use simplifies the methodology of the determination of water and electrolyte contents in the cells.
D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D001001:Anura; D001834:Body Water / Q000378:metabolism; D003035:Cobalt / Q000378:metabolism*; D003037:Cobalt Radioisotopes; D004492:Edetic Acid / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism*; D007444:Inulin / Q000378:metabolism; D010042:Ouabain; D011188:Potassium / Q000378:metabolism; D011898:Ranidae; D012867:Skin / Q000187:drug effects / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism; D014667:Vasopressins / Q000494:pharmacology
D003037:Cobalt Radioisotopes; D014667:Vasopressins; D003035:Cobalt; D010042:Ouabain; D000584:Amiloride; D007444:Inulin; D004492:Edetic Acid; D012964:Sodium; D011188:Potassium