title: Transduction of Myxococcus virescens by coliphage P1CM: generation of plasmids containing both phage and Myxococcus genes.
abstract: Chloramphenicol-resistant Myxococcus virescens were obtained by infecting myxococci with Escherichia coli specialized transducing phage P1CM. The drug-resistant myxococci were phenotypically unstable. They contained more than one type of plasmid; these plasmids were not found in the parent strain. Chloramphenicol-resistant E. coli were obtained by transformation with either a fraction of myxococcal DNA containing the plasmids or with P1CM prophage DNA. These transformants contained plasmids. Escherichia coli transformed by DNA from the myxococci contained both P1CM and myxococcal genes. Individual transformant clones differed in the genetic make-up of their plasmids. Among the myxococcal genes expressed in these plasmid-harbouring E. coli strains were a capacity for self-transmissibility and a pattern of phage sensitivity characteristic of R factor incompatibility group W. Escherichia coli transformed with P1CM prophage contained incomplete P1CM genomes; none of the chloramphenicol-resistant transformants produced P1CM phage particles. The significance of these findings for an understanding of mechanisms for the generation of R factors is discussed.
mesh_terms: D002701:Chloramphenicol / Q000494:pharmacology; D003090:Coliphages / Q000235:genetics*; D004269:DNA, Bacterial; D004279:DNA, Viral; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D009231:Myxococcales / Q000187:drug effects / Q000235:genetics*; D010641:Phenotype; D010957:Plasmids; D014161:Transduction, Genetic
chemical_list: D004269:DNA, Bacterial; D004279:DNA, Viral; D002701:Chloramphenicol