Determination of free thyroid hormone fractions using a simple T3-uptake test.
Results from a simple, fast and inexpensive Sephadex T3-uptake test (T3U) have been compared to free fractions of serum thyroxine (T4) and triiodothyronine (T3) determined by a dialysis procedure in forty-six sera from hypothyroids, 154 sera from euthyroids and ninety-one sera from hyperthyroids. Sera with high TBG levels were included in these groups. A second order correlation (r = 0.90) was found between T3U and free T4 and T3 fractions. The T3U had a low diagnostic value for all three groups. Free hormone indices calculated as the product between the T4 or T3 and T3U were linearly correlated to total free T4 (r = 0.94) and total free T3 (r = 0.96) if these are less than 2.5 times the upper nu described.
D003956:Dialysis; D006801:Humans; D006980:Hyperthyroidism / Q000175:diagnosis; D007037:Hypothyroidism / Q000175:diagnosis; D008722:Methods; D013974:Thyroxine / Q000097:blood*; D013975:Thyroxine-Binding Proteins / Q000032:analysis; D014284:Triiodothyronine / Q000097:blood
D013975:Thyroxine-Binding Proteins; D014284:Triiodothyronine; D013974:Thyroxine