Combined autoradiography and histochemistry: the simultaneous detection of 6-3H thymidine and acid phosphatase activity in cryostate sections.
A method is described for the simultaneous demonstration of 6-3H thymidine incorporation and acid phosphatase activity in cryostae sections of mouse thymus. The method enables a comparison of mitosis and acid hydrolase activity to be made in the same tissue section. In 8-week-old mice acid phosphatase positive cells reprsent 1.23 +/- 0.06% of the total population and 8.4 +/- 0.27% of the cells incorporate tritiated thymidine. Acid phosphatase activity can be used to estimate cell autolysis and death. The implication of the method in relation to tissue dynamics is discussed.
D000135:Acid Phosphatase / Q000502:physiology*; D000818:Animals; D001345:Autoradiography; D002470:Cell Survival; D005615:Freezing; D006651:Histocytochemistry; D051379:Mice; D008853:Microscopy; D008938:Mitosis; D013194:Staining and Labeling / Q000379:methods; D013936:Thymidine / Q000502:physiology*; D013950:Thymus Gland / Q000166:cytology*; D014316:Tritium
D014316:Tritium; D000135:Acid Phosphatase; D013936:Thymidine