title: [Riboflavin biosynthesis operon of Bacillus subtilis. XIV. Operator-constitutive mutants].
abstract: Numerous operator-constitutive mutants of riboflavin biosynthesis were selected. All of them map in a short region of the Bacillus subtilis chromosome. The yield of riboflavin synthetase from this mutant is different, but in most cases much lower than the maximal yield from a repressor minus strain. Our tentative explanation is a partial overlap of the sites for the adsorption of repressor and RNA-polymerase. Therefore the affinity to the transcribing enzyme is diminished in the operator constitutive strains. The affinity of the repressor-effector complex to the operator depends on the effector structure.
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics*; D005809:Genes, Regulator; D009154:Mutation; D009876:Operon; D012256:Riboflavin / Q000235:genetics*; D012258:Riboflavin Synthase / Q000235:genetics; D014169:Transformation, Bacterial
chemical_list: D012258:Riboflavin Synthase; D012256:Riboflavin
vernacular_title: Issledovanie operona biosinteza riboflavina u Bacillus subtilis. Soobshchenie XIV. Izuchenie operator-konstitutivnykh mutantov.