Histochemical technique to detect choline-containing lipids.
An almost specific and highly sensitive technique to detect histochemically choline-containing lipids is proposed. This technique is based on the effect of Hg++ and phosphomolybdic ion on the choline, that yields insoluble complexes able to react with diphenylcarbazide producing a deep blue or violet stain. The specificity and the sensitivity of this technique was investigated on filter paper strips loaded with choline-containing lipids, choline-free lipids, steroids, vitamins, proteins or choline hydrochloride. The results show that the choline-containing substances react positively. The choline-free substances react negatively except calciferol and vitamin A that show weakly positive results. This technique used on tissue sections appears very suitable to histochemical purposes.
D000311:Adrenal Glands / Q000032:analysis; D000818:Animals; D001923:Brain Chemistry; D002415:Cats; D002794:Choline / Q000032:analysis*; D004285:Dogs; D006651:Histocytochemistry / Q000379:methods*; D008055:Lipids / Q000032:analysis*; D008099:Liver / Q000032:analysis; D011817:Rabbits; D051381:Rats; D011934:Reagent Strips; D012584:Sciatic Nerve / Q000032:analysis; D012867:Skin / Q000032:analysis
D008055:Lipids; D011934:Reagent Strips; D002794:Choline