13C NMR analysis of methionine sulfoxide in protein.
The 13C epsilon NMR signal of methionine sulfoxide is 22.6 ppm downfield from that of methionine. This affords a method by which the extent of methionine oxidation can be determined in intact protein. We demonstrate the utility of this approach with beta-galactosidase enriched with 13C in its methionine methyls.
D002700:Chloramines; D004926:Escherichia coli / Q000201:enzymology; D009682:Magnetic Resonance Spectroscopy; D008715:Methionine / Q000031:analogs & derivatives* / Q000032:analysis; D010084:Oxidation-Reduction; D011506:Proteins; D013454:Sulfoxides / Q000032:analysis; D014105:Tosyl Compounds; D001616:beta-Galactosidase
D002700:Chloramines; D011506:Proteins; D013454:Sulfoxides; D014105:Tosyl Compounds; C016300:chloramine-T; D008715:Methionine; D001616:beta-Galactosidase; C013111:methionine sulfoxide