In vivo activation of murine peritoneal macrophages by Nocardia water soluble mitogen (NWSM).
In vitro NWSM has been shown to be a B cell mitogen and a polyclonal activator. Although NWSM has been also shown to be adjuvant active in vivo, motogenicity and polyclonal activation are not observed when this fraction has been administered to mice. In contrast, the experiments reported here demonstrate that after in vivo administration several effects of NWSM on mouse peritoneal macrophages can be observed: NWSM was able to induce an important increase in the ability of peritoneal macrophages to inhibit in vitro growth of tumor cells, to increase their phagocytic activity and to enhance their ability to induce an immune response, following the incubation with an antigen NWSM was able to stimulate phagocytic activity of macrophages of C3H/He Orl. mice (LPS-resistant strain). Those findings suggest that the adjuvant activity of NWSM in vivo can be related to its capacity to activate macrophages.
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D000941:Antigens; D002455:Cell Division; D002471:Cell Transformation, Neoplastic; D002645:Chickens; D004912:Erythrocytes / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology*; D012515:Mast-Cell Sarcoma / Q000276:immunology; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008934:Mitogens / Q000494:pharmacology*; D009161:Mycobacterium / Q000276:immunology; D009615:Nocardia / Q000276:immunology*; D010587:Phagocytosis; D012756:Sheep
D000276:Adjuvants, Immunologic; D000941:Antigens; D008070:Lipopolysaccharides; D008934:Mitogens