title: Identification of chorion protein precursors and the mRNAs that encode them in Drosophila melanogaster.
abstract: Specific antisera were generated for major size classes of Drosophila chorion proteins. These antisera were used in conjunction with cell-free translation of follicular RNA to identify precursors, containing signal peptides, for chorion components A1, A2, B1, and B2. An additional precursor cross-reacts with anti-B antisera, although its product appears to correspond to a protein distinct from authenic B proteins. The size classes of mRNA encoding A and B protein precursors have been identified by cell-free translations.
mesh_terms: D000818:Animals; D002474:Cell-Free System; D002823:Chorion / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000235:genetics*; D004527:Egg Proteins / Q000235:genetics*; D005260:Female; D007158:Immunologic Techniques; D007425:Intracellular Membranes / Q000378:metabolism; D008861:Microsomes / Q000378:metabolism; D008970:Molecular Weight; D009866:Oogenesis; D010053:Ovary / Q000378:metabolism; D011498:Protein Precursors / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D004527:Egg Proteins; D011498:Protein Precursors; D012333:RNA, Messenger