[Kinetic study of membrane 5'-nucleotidase activity in cultured diploid cells].
5'-nucleotidase activity of cultured diploïd cells was measured using intact suspended cells. This depended on the substrate amount, but did not vary with Mg ++ concentration-Kinetic data were determined using Con. A,a specific inhibitor.
D000249:Adenosine Monophosphate / Q000494:pharmacology; D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000201:enzymology*; D002478:Cells, Cultured; D002610:Cheek / Q000166:cytology / Q000196:embryology; D003208:Concanavalin A / Q000494:pharmacology; D003470:Culture Media; D004171:Diploidy; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D009708:Nucleotidases / Q000037:antagonists & inhibitors / Q000378:metabolism*
D003470:Culture Media; D003208:Concanavalin A; D000249:Adenosine Monophosphate; D009708:Nucleotidases; D008274:Magnesium
Etude cinétique de l'activité 5'-nucléotidase membranaire de cellules diploïdes en culture.