Patterns of reversion to bobbed condition of magnified bobbed loci in D. melanogaster.
The number of genes coding for the ribosomal RNA (rDNA) can increase in D. melanogaster by means of a process called magnification. In this way, a partial deletion in this locus, termed bobbed, can reach a wild type condition. A newly magnified locus, in turn, reverts to a deficient bobbed condition if it is kept in a phenotypically wild type genotype for several generations. We studied bobbed loci at different magnification steps, analysing their behaviour through the reversion process and the way they carry out a second round of magnification. Results based on the analysis of the reversion process led to the conclusion that magnification consists of a progressive integration into the bobbed locus of free rDNA copies. Moreover, evidence is supplied that the extent of this integration affects the way a reverted locus goes through a second magnification cycle. The extensive characterization of reverted bobbed loci lends substantial support to the extra copies model of rDNA magnification.
D000818:Animals; D004331:Drosophila melanogaster / Q000235:genetics*; D005784:Gene Amplification; D005796:Genes; D009154:Mutation; D010641:Phenotype; D012335:RNA, Ribosomal / Q000235:genetics*
D012335:RNA, Ribosomal