Evaluation of several enrichment procedures for the isolation of recombinant plasmid DNA.
A number of methods for the selective enrichment of recombinant plasmids were examined; these include alkaline phosphatase treatment of the restricted pBR322 vector, as well as a combination of this and S1 nuclease treatment of the ligated mixture of pBR322 and pCR1 plasmids or S. griseus DNA followed by D-cycloserine treatment to enrich for cells carrying recombinant molecules. The relative efficiencies of these methods were compared.
D000469:Alkaline Phosphatase / Q000494:pharmacology; D003523:Cycloserine / Q000494:pharmacology; D004274:DNA, Recombinant / Q000302:isolation & purification*; D004926:Escherichia coli / Q000187:drug effects; D008722:Methods; D010957:Plasmids; D013305:Streptomyces griseus; D013752:Tetracycline / Q000494:pharmacology
D004274:DNA, Recombinant; D003523:Cycloserine; D000469:Alkaline Phosphatase; D013752:Tetracycline