Formation and characterization of antibody against 2'-(5"-phosphoribosyl)-5' AMP, the monomer form of poly(adenosine diphosphate ribose).
Specific antibody against 2'-(5"-phosphoribosyl)-5'AMP (PR-AMP), a monomer of poly(adenosine diphosphate ribose) (poly(ADP-Rib)), was produced by immunizing a rabbit with PR-AMP coupled to bovine serum albumin (BSA). Antibody against PR-AMP was purified 53-fold from serum by (NH4) 2SO4 precipitation, and BSA-Sepharose 4B, DEAE-cellulose and (PR-AMP)-BSA-Sepharose 4B column chromatographies. Inhibition experiments show that the adenine ring, 5'-phosphate residue and ribose-ribose bond of PR-AMP were essential for the antigenic determinant of PR-AMP. Anti PR-AMP antibody bound, not only with PR-AMP, but also with poly(ADP-Rib) of various chain lengths, while anti poly(ADP-Rib) antibody bound with poly(ADP-Rib) but not with PR-AMP.
D000249:Adenosine Monophosphate / Q000031:analogs & derivatives* / Q000276:immunology; D000818:Animals; D000917:Antibody Formation; D003429:Cross Reactions; D007106:Immune Sera; D007118:Immunoassay; D005779:Immunodiffusion; D009702:Nucleoside Diphosphate Sugars; D011064:Poly Adenosine Diphosphate Ribose; D011817:Rabbits / Q000276:immunology; D012267:Ribosemonophosphates / Q000276:immunology
D007106:Immune Sera; D009702:Nucleoside Diphosphate Sugars; D012267:Ribosemonophosphates; D011064:Poly Adenosine Diphosphate Ribose; D000249:Adenosine Monophosphate