Effects of low dietary protein concentration and energy deprivation on lymphocyte transformation in melanoma-bearing mice.
Temporal changes in lymphocyte blastogenesis were studied using spleen cells from syngeneic melanoma-bearing and control mice fed various levels of purified diets containing 6, 10 or 30% casein. T-cell blastogenesis was stimulated by the presence of the tumor and these responses changed with the duration of feeding. In addition, protein concentration did not affect T-cell transformation but the level of energy intake influenced concanavalin A induced DNA synthesis. In contrast, the growing melanoma did not influence B-cell transformation whereas a very low level of dietary protein, a low level of energy intake and duration of the dietary manipulation influenced these cells. Tumor weights were generally not affected by the diet except in mice receiving a very low level of energy intake. Thus, we have found that B-cell responses were affected more than those of T-cells and that moderate protein deficiency did not enhance cellular immune responses in syngeneic tumor-bearing and control mice.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D004044:Dietary Proteins / Q000008:administration & dosage*; D005260:Female; D008213:Lymphocyte Activation; D008545:Melanoma / Q000276:immunology*; D051379:Mice; D009368:Neoplasm Transplantation; D011502:Protein-Energy Malnutrition / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
D004044:Dietary Proteins