Manual solid phase sequence analysis of polypeptides using 4-N-N,-dimethylaminoazobenzene 4'-isothiocyanate.
A manual solid-phase method for sequence analysis of polypeptides is described. The immobilized polypeptide was subjected to stepwise degradation by Edman-type reagent, using the 4-N,N-dimethylaminoazobenzene 4'-isothiocyanate phenyllisothiocyanate double coupling method. The N-terminal amino acids were released (after conversion reaction) as 4-N,N-dimethylaminoazobenzene 4'-thiohydantoin (identified by thin layer chromatography) and phenylthiohydantoin derivatives. The method required 2--10 nmol polypeptide.
D000595:Amino Acid Sequence; D001322:Autoanalysis / Q000379:methods; D004926:Escherichia coli; D010455:Peptides / Q000032:analysis*; D012269:Ribosomal Proteins / Q000032:analysis; D012995:Solubility; D013861:Thiocyanates; D004124:p-Dimethylaminoazobenzene / Q000031:analogs & derivatives
D010455:Peptides; D012269:Ribosomal Proteins; D013861:Thiocyanates; D004124:p-Dimethylaminoazobenzene