[Kinetic determination of alpha-amylase in serum and urine with an oligosaccharide as substrate--modification for a fully mechanized enzyme measuring device (author's transl)].
The author reports a modification of the UV method UltraZyme Plus alpha-Amyl Harleco and the adaptation to the Eppendorf Enzymautomat 5010. alpha-amylase acts on an oligosaccharide mixture yielding maltose, which is hydrolysed by alpha-glucosidase. The liberated glucose is determined specifically by the hexokinase/glucose-6-phosphate dehydrogenase (NAD+-dependent) method+ by addition of pyruvate, lactate dehydrogenase and ATP. Thereafter the lactate dehydrogenase reaction is stopped by addition of oxamate and the alpha-amylase activity is measured.
D000681:Amylases / Q000378:metabolism*; D005947:Glucose / Q000032:analysis; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005959:Glucosidases / Q000378:metabolism; D006593:Hexokinase / Q000378:metabolism; D006801:Humans; D008320:Maltose / Q000096:biosynthesis; D008722:Methods; D009243:NAD / Q000096:biosynthesis / Q000378:metabolism; D009844:Oligosaccharides / Q000378:metabolism*; D010072:Oxamic Acid; D000516:alpha-Amylases / Q000097:blood / Q000378:metabolism* / Q000652:urine
D009844:Oligosaccharides; D009243:NAD; D008320:Maltose; D005954:Glucosephosphate Dehydrogenase; D006593:Hexokinase; D000681:Amylases; D005959:Glucosidases; D000516:alpha-Amylases; D005947:Glucose; D010072:Oxamic Acid
Die kinetische Bestimmung der alpha-Amylase im Serum un Urin mit einem Oligosaccharid als Substrate Modifikation für einen vollmechaniserten Enzymmessplatz.