title: Late transcription and simultaneous replication of simian adenovirus 7 DNA as revealed by spreading lytically infected cell cultures.
abstract: Miller's technique of spreading DNA was applied to monkey cells productively infected with simian adenovirus 7. This permitted the visualization of cellular DNA transcription, both nucleolar and non-nucleolar, and of late transcription and replication of virus. Virus double-stranded DNA, thin fibres with very few nucleosome-like particles, were observed carrying either transcription or replication complexes. In addition, both RNP transcripts and replication forks were found on some virus duplex DNA. Virus single-stranded DNA replicative intermediates were identified on the basis of their increased thickness and contrast which results from the presence of a DNA binding protein.
mesh_terms: D000256:Adenoviridae / Q000378:metabolism*; D000261:Adenoviruses, Simian / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D002521:Cercopithecus; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis*; D000882:Haplorhini; D007668:Kidney; D008854:Microscopy, Electron; D014158:Transcription, Genetic
chemical_list: D004279:DNA, Viral