A sensitive enzyme immunoassay for the quantitation of human IgM.
A highly sensitive and specific two-site solid-phase enzyme immunoassay for the quantitation of human immunoglobulin M (IgM) in biological fluids is presented. Detection limit is 20 micrograms/l (12 ng/tube). Routine working range is 30--1000 micrograms/l. Between-assay coefficient of variation is 10%. The assay allows quantitation of IgM in human cerebrospinal fluid.
D001826:Body Fluids / Q000032:analysis; D006801:Humans; D005779:Immunodiffusion; D007123:Immunoelectrophoresis, Two-Dimensional; D007124:Immunoenzyme Techniques; D007075:Immunoglobulin M / Q000032:analysis* / Q000134:cerebrospinal fluid; D009101:Multiple Myeloma / Q000276:immunology; D008258:Waldenstrom Macroglobulinemia / Q000276:immunology
D007075:Immunoglobulin M