Aminotripeptidase, a cytosol enzyme from rabbit intestinal mucosa.
Aminotripeptidase, a cytosol enzyme from rabbit intestinal mucosa, was purified to homogeneity. The pure enzyme is a glycoprotein containing a very small amount of sugar. It is composed of only one subunit of 50,000 mol. wt. and possesses 1 zinc atom per molecule. Its specificity is primarily directed towards tripeptides with an N-terminal proline residue. However, the enzyme is also able to hydrolyse other tripeptides, except those with either a charged amino acid in the N-terminal position or a proline residue in the second position. The purified aminotripeptidase accounts for almost all the tripeptidase activity of the soluble fraction from intestinal mucosa.
D000626:Aminopeptidases / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D003600:Cytosol / Q000201:enzymology; D004492:Edetic Acid / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D007413:Intestinal Mucosa / Q000201:enzymology*; D007700:Kinetics; D011817:Rabbits; D013379:Substrate Specificity; D015032:Zinc / Q000032:analysis
D002241:Carbohydrates; D004492:Edetic Acid; D000626:Aminopeptidases; D015032:Zinc