[Isolation and immunochemical study of the toxic complex of Cl. botulinum type F].
The toxic comples of Cl. botulinum, type F, was separated into the toxic and nontoxic protein fractions by the methods of ion exchange chromatography and gel filtration in accordance with a specially devised purification scheme. Highly purified, electrophoretically and serologically homogeneous toxin with a molecular weight of 150,000 and potency equal to 10 X 10(6) DLM per 1 mg of protein was isolated from the toxic fraction. The nontoxic protein component had faintly pronounced hemagglutinating properties and was essentially different from type A and B hemagglutinins. The toxic complex of Cl. botulinum, type F, was shown to contain a proteolytically active fraction.
D000942:Antigens, Bacterial / Q000032:analysis; D001426:Bacterial Proteins / Q000032:analysis; D001905:Botulinum Toxins / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D003014:Clostridium botulinum / Q000276:immunology*; D004912:Erythrocytes / Q000276:immunology; D006386:Hemagglutination Tests; D006801:Humans; D005779:Immunodiffusion; D066298:In Vitro Techniques
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001905:Botulinum Toxins
Poluchenie i immunokhimicheskoe issledovanie toksicheskogo kompleksa Cl. botulinum tipa F.