[Ultrastructural localization of 3-beta-hydroxysteroid dehydrogenase activity in the glandular tissue cells of the testis of Pleurodeles waltlii Michach. (Amphibia, Urodela)].
With the potassium ferricyanide method, the localization of the sites of 3 beta-hydroxysteroid dehydrogenase activity has been investigated in the glandular tissue cells, previously fixed, of the Pleurodeles testis. An abundant copper ferrocyanide precipitate, the final reaction product, is observed in the vicinity of the external faces of the membranes of the smooth endoplasmic reticulum, or in contact with them. A very weak reaction occurs in mitochondrial cristae.
D015096:3-Hydroxysteroid Dehydrogenases / Q000032:analysis*; D000663:Amphibians / Q000378:metabolism*; D000818:Animals; D004721:Endoplasmic Reticulum / Q000201:enzymology; D007425:Intracellular Membranes / Q000201:enzymology; D008297:Male; D011540:Prussian Blue Reaction; D013737:Testis / Q000201:enzymology* / Q000648:ultrastructure
D015096:3-Hydroxysteroid Dehydrogenases
Localisation ultrastructurale de l'activité 3 beta-hydroxystéroïde déshydrogénasique dans les cellules du tissu glandulaire du testicule de Pleurodeles waltlii Michah. (Amphibien, Urodèle).