[Isolation of antilymphocytic antibodies with the aid of cellular immunosorbents].
The authors suggest a method of obtaining purified antilymphocytic antibodies by mean of a specific immunosorbent of human or mouse lymphocytes fixated with glutharic aldehyde Such immunosorbents subjected to special treatment could be used repeatedly; their sorptive capacity was retained in such case. Only from 5 to 12% of the activity could be obtained from immunosorbents sorbed from the serum with 77--93% activity. In comparison with the initial serum, purification was from 6- to 15-fold. Thus, the suggested method provided considerable purification of the antilymphocytic preparations and permitted to obtain highly active antilymphocytic antibodies.
D000447:Aldehydes / Q000494:pharmacology*; D000818:Animals; D000936:Antigen-Antibody Complex / Q000494:pharmacology*; D001323:Autoantibodies / Q000302:isolation & purification*; D003601:Cytotoxicity Tests, Immunologic; D005976:Glutaral / Q000494:pharmacology*; D006801:Humans; D007163:Immunosorbent Techniques; D007164:Immunosorbents / Q000494:pharmacology*; D008214:Lymphocytes / Q000276:immunology*; D008233:Lymphotoxin-alpha / Q000302:isolation & purification*; D051379:Mice; D014066:Palatine Tonsil / Q000166:cytology; D013950:Thymus Gland / Q000166:cytology
D000447:Aldehydes; D000936:Antigen-Antibody Complex; D001323:Autoantibodies; D007164:Immunosorbents; D008233:Lymphotoxin-alpha; D005976:Glutaral
Vydelenia antilimfotsitarnykh antitel pri pomoshchi kletochnykh immunosorbentov.