Studies on the transfer of protective immunity with lymphoid cells from mice immune to malaria sporozoites.
In an effort to understand the mechanisms involved in the protective immunity to malarial sporozoites, an A/J mouse/Plasmodium berghei model was studied. Protective immunity could consistently be adoptively transferred only by using sublethal irradiation of recipients (500 R); a spleen equivalent (100 X 10(6))of donor cells from immune syngeneic mice; and a small booster immunization (1 X 10(4)) of recipients with irradiation-attenuated sporozoites. Recipient animals treated in this manner were protected from lethal challenge with 1 X 10(4) nonattenuated sporozoites. Immune and nonimmune serum and spleen cells from nonimmune animals did not protect recipient mice. Fewer immune spleen cells (50 X 10(6)) protected some recipients. In vitro treatment of immune spleen cells with anti-theta sera and complement abolished their ability to transfer protection. This preliminary study suggests that protective sporozoite immunity can be transferred with cells, and that it is T cell dependent.
D000818:Animals; D000961:Antilymphocyte Serum / Q000494:pharmacology; D016782:Apicomplexa / Q000276:immunology; D005260:Female; D007116:Immunization, Passive; D008214:Lymphocytes / Q000276:immunology*; D008288:Malaria / Q000517:prevention & control*; D051379:Mice; D008805:Mice, Inbred A; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D000961:Antilymphocyte Serum