title: [Immuno-electro-diffusion on cellulose acetate membrane for serological testing in human malaria (author's transl)].
abstract: An immuno-electro-diffusion assay for human malaria, using cellulose acetate gel, is described. More than 3500 sera have been tested by this method, which proved very useful for epidemiology since 400 sera a day can be tested by one person, with simple equipment. Its sensitivity compared well to the IFA test, although the antibodies revealed are quite different. A constnat precipitin line appeared, against 56 degrees C labile antigens, in sera of subjects infected by P. falciparum, and up to 8 other precipitins per serum tested. The test may be useful in analyzing these multiple antibodies occurring in patients with malaria.
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000941:Antigens / Q000302:isolation & purification; D002648:Child; D002675:Child, Preschool; D004812:Epidemiologic Methods; D006801:Humans; D005779:Immunodiffusion / Q000379:methods; D007122:Immunoelectrophoresis / Q000379:methods; D008288:Malaria / Q000175:diagnosis* / Q000276:immunology; D008875:Middle Aged; D010963:Plasmodium falciparum / Q000276:immunology
chemical_list: D000941:Antigens
vernacular_title: L'immuno-électro-diffusion sur membrane d'acétate de cellulose pour le diagnostic sérologique du paludisme humain.