Mutants of Bacillus subtilis affected in spore outgrowth.
Six mutants of Bacillus subtilis 168 that are temperature-sensitive in spore outgrowth were isolated. The outgrowth process proceeds normally at 35 degrees C, but at the non-permissive temperature (47 degrees C) it is arrested at a specific stage characteristic for each mutant strain. The mutants are not altered in vegetative growth whether at 35 degrees C or at 47 degrees C. They were characterized for their ability to synthesize RNA, proteins and DNA during outgrowth. A mutant defective in spore germination was also isolated; less than 5% of its spores can germinate at any of the temperatures tested. The mutations were mapped by means of transduction and transformation. The isolation of a number of outgrowth mutants which map at different loci and which affect outgrowth at different times is discussed in relation to the regulation of this process.
D001412:Bacillus subtilis / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D001426:Bacterial Proteins / Q000096:biosynthesis; D002874:Chromosome Mapping; D004269:DNA, Bacterial / Q000096:biosynthesis; D046911:Macromolecular Substances; D009024:Morphogenesis; D009154:Mutation; D012329:RNA, Bacterial / Q000096:biosynthesis; D013171:Spores, Bacterial; D013696:Temperature
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D046911:Macromolecular Substances; D012329:RNA, Bacterial