Convenient method for studying enzyme kinetics.
A convenient method for enzyme kinetic studies is introduced. The method includes identification of reaction mechanism and estimation of the associated kinetic constants with a minimum number of experiments. The application of the method is illustrated by using literature data. Factors limiting the application of this method are also discussed.
D001667:Binding, Competitive; D002475:Cellobiose / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004798:Enzymes / Q000378:metabolism*; D005947:Glucose / Q000096:biosynthesis; D006868:Hydrolysis; D007700:Kinetics; D008956:Models, Chemical; D001617:beta-Glucosidase / Q000378:metabolism
D004791:Enzyme Inhibitors; D004798:Enzymes; D002475:Cellobiose; D001617:beta-Glucosidase; D005947:Glucose