Histochemical and chronobiological studies on uptake of L-DOPA by granulocytes--its promotion and suppression.
The uptake capacity of granulocytes for L-DOPA was investigated from the aspect of histochemistry and chronobiology. For a histochemical research, modified FALCK-HILLARP method for catecholamine, CORRODI's test for identification of L-DOPA and an electron microscopical survey of glycogen particles were carried out. For a chronobiological research, daily and seasonal changes in the uptake of L-DOPA by granulocytes were examined with blood smear method of the authors. From this experiment, following points became clear; 1. The uptake capacity of granulocytes for L-DOPA varies with a clock-time and a season judging from fluorescent intensity and tincture of granulocytes. 2. The uptake capacity of granulocytes for L-DOPA was suppressed by major tranquilizers especially haloperidol, and by lithium carbonate, while the uptake was enhanced by prednisolone, dexamethasone, MK-486, and dibutyryl cyclic AMP. 3. The electron opacity of glycogen particles in granulocytes weakened significantly after the administration of L-DOPA. Isoproterenol had also a similar effect, but the effect of the former was superior to that of the latter under the authors' conditions. Haloperidol inhibited strongly the decrease of glycogen caused by L-DOPA. 4. DOPAmine was hardly uptaken by granulocytes in general. Glycogen in granulocytes did not show a marked decrease after the administration of DOPAmine. 5. From the evidence mentioned above, glycogen content in granulocytes was assumed to be closely associated with the uptake of monoamine by granulocytes.
D000818:Animals; D004298:Dopamine / Q000097:blood; D006003:Glycogen / Q000097:blood; D006098:Granulocytes / Q000187:drug effects / Q000378:metabolism*; D006220:Haloperidol / Q000494:pharmacology; D006728:Hormones / Q000494:pharmacology; D007980:Levodopa / Q000097:blood*; D008297:Male; D051381:Rats; D012621:Seasons; D013256:Steroids / Q000494:pharmacology; D014149:Tranquilizing Agents / Q000494:pharmacology
D006728:Hormones; D013256:Steroids; D014149:Tranquilizing Agents; D007980:Levodopa; D006003:Glycogen; D006220:Haloperidol; D004298:Dopamine