Differential sensitivity of memory cell subpopulations to anti-immunoglobulin and complement.
Evidence is presented for the unique sensitivity of memory cells bearing surface immunoglobulin G1 (IgG1) to functional elimination with anti-immunoglobulin (anti-Ig) sera and complement (C). Treatment of cells for adoptive transfer with C and anti-gamma1, anti-kappa, or anti-Ig significantly reduces the number of plaque-forming cells (PFC) of only the IgG1 isotype found in adoptive recipients. An increase in PFC of other isotypes accompanies the decrease in IgG1 PFC; there is no net change in the total PFC response. The depletion of IgG1 PFC requires treatment of transferred cells with both specific antisera and C; antisera directed against other isotypes show no significant effects. The maintenance of the magnitude of PFC response, compensation, is discussed.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D000917:Antibody Formation; D000937:Antigen-Antibody Reactions; D002999:Clone Cells / Q000276:immunology*; D003165:Complement System Proteins / Q000378:metabolism; D004306:Dose-Response Relationship, Immunologic; D007126:Immunoglobulin Allotypes; D007142:Immunoglobulin gamma-Chains; D007145:Immunoglobulin kappa-Chains; D007156:Immunologic Memory; D008297:Male; D051379:Mice; D013154:Spleen / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D007126:Immunoglobulin Allotypes; D007142:Immunoglobulin gamma-Chains; D007145:Immunoglobulin kappa-Chains; D003165:Complement System Proteins