Inhibition of the cytochrome P-450 multifunctional oxidase by N-propargyl analogs of SKF-525 A and acetylmethadol.
New N-propargyl analogs of SKF-525A and acetyl methadol were tested for their inhibitory potency toward P-450 multifunctional oxidase. Metabolism of these analogs appears to be different from the metabolism of SKF-525 A itself; no N-dealkylated species were detected, --in vitro N-demethylation of aminopyrine and spectrofluorometric measurements support the hypothesis of an interaction between the hepatic flavo-protein and the acetylenic function of the inhibitors.
D000633:Aminopyrine N-Demethylase / Q000032:analysis; D000818:Animals; D065607:Cytochrome P-450 Enzyme Inhibitors; D005260:Female; D005415:Flavins / Q000494:pharmacology; D005420:Flavoproteins; D005470:Fluorometry; D007700:Kinetics; D008297:Male; D008691:Methadone / Q000031:analogs & derivatives*; D008692:Methadyl Acetate / Q000031:analogs & derivatives* / Q000494:pharmacology*; D006899:Mixed Function Oxygenases / Q000037:antagonists & inhibitors*; D010088:Oxidoreductases / Q000037:antagonists & inhibitors*; D011335:Proadifen / Q000031:analogs & derivatives* / Q000494:pharmacology*; D011817:Rabbits; D051381:Rats; D013997:Time Factors
D065607:Cytochrome P-450 Enzyme Inhibitors; D005415:Flavins; D005420:Flavoproteins; D011335:Proadifen; D006899:Mixed Function Oxygenases; D010088:Oxidoreductases; D000633:Aminopyrine N-Demethylase; D008692:Methadyl Acetate; D008691:Methadone