Differentiation of mycobacteria by "in vivo" and "in vitro" delayed hypersensitivity reactions.
Guinea pigs were sensitized with heat killed M. tuberculosis, M. kansasii, M. scrofulaceum, and M. avium-intracellulare. Purified Protein Derivatives (PPDs) prepared from each of these organisms were tested for delayed type hypersensitivity by lymphocyte transformation, macrophage migration, and mantoux type skin sensitivity tests. The homologous reactions were consistently more intense than heterologous reactions in all three procedures. The skin test procedure was more sensitive than either in vitro method. Positive  skin test reactions were elicited with 2 times and 50 times less antigen than, respectively, the lymphocyte transformation and the macrophage migration tests.
D000818:Animals; D000942:Antigens, Bacterial; D001426:Bacterial Proteins / Q000276:immunology; D002464:Cell Migration Inhibition; D006168:Guinea Pigs; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D008263:Macrophage Migration-Inhibitory Factors; D009161:Mycobacterium / Q000276:immunology*; D009162:Mycobacterium avium / Q000276:immunology; D009169:Mycobacterium tuberculosis / Q000276:immunology; D012882:Skin Tests
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D008263:Macrophage Migration-Inhibitory Factors