[Purification of beta-glucanase on cellulose].
The paper describes a method for purifying beta-glucanse (lychenase) which includes enzyme absorption on the microcrystalline cellulose column or ground filter paper at pH 5.5, and elution with phosphate buffer pH 7.6. Specific activity of beta-glucanase eluted from the column may be increased by 8--23 times, depending on the purification conditions.
D001412:Bacillus subtilis / Q000201:enzymology; D002482:Cellulose; D005959:Glucosidases / Q000302:isolation & purification*; D007700:Kinetics; D008011:Lichens / Q000201:enzymology; D010944:Plants / Q000201:enzymology; D001617:beta-Glucosidase / Q000302:isolation & purification* / Q000378:metabolism
D002482:Cellulose; D005959:Glucosidases; D001617:beta-Glucosidase
Ochistka beta-gliukanazy na tselliuloze.