Effect of 3-methylcholanthrene on the biliary excretion of bromsulphthalein and eosine in newborn rats.
The biliary excretion rates of bromsulphthalein (BSP), bromsulphthalein-glutathione conjugate (BSP-GSH) and eosine have been studied in 3-methylcholanthrene (3-MC)-pretreated (100 mg/kg i.p.) and control rats aged 10 days. Liver weight was invariably increased after 3-MC treatment, associated with enhanced biliary excretion of total BSP. The increase in the biliary excretion of total BSP was due solely to the increased excretion of BSP-GSH. Following 3-MC pretreatment, BSP-GSH and eosine appeared in the bile in the same amount as in the control rats after i.v. administration of BSP-GSH and eosine. Pretreatment with 3-MC increased the ratio of BSP-GSH to BSP in the liver and bile. Our results suggest that the increased biliary excretion of total BSP following 3-MC treatment was due to an enhanced conjugation of BSP with GSH.
D000818:Animals; D000831:Animals, Newborn; D001646:Bile / Q000032:analysis; D001659:Biliary Tract / Q000187:drug effects* / Q000378:metabolism; D004801:Eosine Yellowish-(YS) / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D007421:Intestine, Small; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008748:Methylcholanthrene / Q000494:pharmacology*; D051381:Rats; D013448:Sulfobromophthalein / Q000378:metabolism*
D013448:Sulfobromophthalein; D008748:Methylcholanthrene; D005978:Glutathione; D004801:Eosine Yellowish-(YS)