[Comparative antiviral and interferonogenic activity of natural and synthetic interferon inducers in vitro and in vivo].
Biological activities of the RNA replicative form of phage f2, a natural interferon inductor and poly-I -- poly-C, a synthetic polyribonucleotide complex were studied comparatively. Differences in the comparative interferonogenic and antiviral activity of the inductors were as dependent on the type of the cell system. It was shown that DEAE-dextran increased the interferon-inducing activity of RFf2 in the cell culture by 4 to 8 times. The dynamics of the interferonogenic and antiviral activity of RFf2 in the L-929 cell culture was studied. Interferon appeared in the culture fluid in 6--8 hours and reached its maximum titers (128 IU50/ml) by the 24th hour, the maximum protection of the cells being also developed by the 12th--24th hour, reaching on an average 51 g PFU/ml. It was shown in the experiments with green marmosets that administration of RFf2 in the form of aerosol in a dose of 2.3 mg/kg induced interferon production in the blood serum the titers of which amounted to 80--160 IU50/ml 24 hours after the administration.
D000818:Animals; D000998:Antiviral Agents; D001435:Bacteriophages; D002478:Cells, Cultured; D003637:DEAE-Dextran / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004666:Encephalitis Virus, Venezuelan Equine / Q000187:drug effects; D000882:Haplorhini; D066298:In Vitro Techniques; D007369:Interferon Inducers / Q000494:pharmacology*; D007372:Interferons / Q000032:analysis; D051379:Mice; D011070:Poly I-C / Q000494:pharmacology; D012367:RNA, Viral / Q000494:pharmacology; D012845:Sindbis Virus / Q000187:drug effects; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D007369:Interferon Inducers; D012367:RNA, Viral; D007372:Interferons; D003637:DEAE-Dextran; D011070:Poly I-C
Sravnitel'naia protivovirusnaia i interferonogennaia aktivnost' prirodnogo i sinteticheskogo induktorov interferona in vitro i in vivo.