Biochemical and morphologic studies on diabetic rats: effects of sucrose-enriched diet in rats with pancreatic islet transplants.
Isolated pancreatic islets were administered to Lewis rats with streptozotocin-induced diabetes. Then the rats were fed either a semisynthetic diet containing 60% (wt/wt) sucrose for 3 weeks or were continued on chow. Transplantation resulted in a decrease in serum glucose, an increase in serum insulin, and a marked decrease in serum triacylglycerol, particularly in the sucrose-fed diabetic rats. In these rats, in demarcated areas of hepatocytes surrounding portal vein termini, lipid was deposited in the cytosol and large lipoprotein particles engorged the Golgi apparatus, Golgi-derived secretory vacuoles, and GERL. This model permits observation of the effects of pancreatic islet hormones on lipogenesis by hepatocytes in situ.
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002784:Cholesterol / Q000097:blood; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D004040:Dietary Carbohydrates / Q000009:adverse effects / Q000378:metabolism*; D005234:Fatty Liver / Q000209:etiology*; D007328:Insulin / Q000097:blood; D016381:Islets of Langerhans Transplantation; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D051381:Rats; D014185:Transplantation, Isogeneic; D014280:Triglycerides / Q000097:blood*
D001786:Blood Glucose; D004040:Dietary Carbohydrates; D007328:Insulin; D014280:Triglycerides; D002784:Cholesterol