[The use of mycobacteria in the estimation of the concentration of antibiotics, antimycotics and antituberculotics (author's transl)].
The possibility of using the rapid resistance test to determine the levels of antibiotic, antimycotic and antituberculotic drugs is systematically investigated. As a rule the incubation time reduced to seven days for tubercel bacilli results in a lower inhibitor concentration (MIC) than with other microbiological methods used in tuberculosis diagnostics due to a lower inactivation of the drugs. The MIC of antibiotics and antituberculotics is found between 0.02 and 15 microgram/ml; for penicillin G and nystatin between 100 and 400 microgram/ml. Urine added to the medium influences the MIC. A dilution of urine 1 : 100 corresponds to the MIC-value found in a control sample with aqua dest. For examining the level of antibiotic drugs in blood a dilution of the blood of at least 1 : 60 is necessary. In this way the levels of rifampicin and isoniazid may be examined. Only 0.1--0.2 ml of capillary blood are needed. If probenecid is administered simultaneously to the patient the rifampicin level in blood is increased for 6 hours. But this effect is small and does not justify a change in the usual dosage of rifampicin.
D000900:Anti-Bacterial Agents / Q000097:blood / Q000494:pharmacology*; D000935:Antifungal Agents / Q000097:blood / Q000494:pharmacology*; D000995:Antitubercular Agents / Q000097:blood / Q000494:pharmacology*; D001414:Bacitracin / Q000494:pharmacology; D004352:Drug Resistance, Microbial / Q000379:methods*; D004977:Ethambutol / Q000494:pharmacology; D005000:Ethionamide / Q000494:pharmacology; D005839:Gentamicins / Q000494:pharmacology; D006801:Humans; D007538:Isoniazid / Q000494:pharmacology; D007612:Kanamycin / Q000494:pharmacology; D009169:Mycobacterium tuberculosis / Q000187:drug effects*; D009355:Neomycin / Q000494:pharmacology; D012293:Rifampin / Q000494:pharmacology; D013307:Streptomycin / Q000494:pharmacology; D013997:Time Factors
D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D000995:Antitubercular Agents; D005839:Gentamicins; D001414:Bacitracin; D007612:Kanamycin; D004977:Ethambutol; D009355:Neomycin; D005000:Ethionamide; D007538:Isoniazid; D012293:Rifampin; D013307:Streptomycin
Zum Einsatz von Mykobakterien bei der Bestimmung der Antibiotika-, Antimykotika- bzw. Antituberkulotika-konzentration.