[Lack of participation of cytoplasmic choline in the synthesis of acetylcholine in the striatal synaptosomes of the rat].
Synaptosomes prepared from Rat striatum were loaded with (14C) choline at 0 degrees C in the course of a 60 min. preincubation. After the removal, by washing, of the extracellular molecules, synaptosomes were incubated 5 min. at 37 degrees C in the presence of (3H) choline. The measurement of the amounts of (3H) ACh and of (14C) ACh formed allowed us to conclude that the acetylation of the molecules of choline was directly coupled to their passage through the synaptosomal membrane.
D000109:Acetylcholine / Q000096:biosynthesis*; D000818:Animals; D002250:Carbon Radioisotopes; D002794:Choline / Q000378:metabolism*; D003342:Corpus Striatum / Q000378:metabolism*; D051381:Rats; D013574:Synaptosomes / Q000378:metabolism*; D014316:Tritium
D002250:Carbon Radioisotopes; D014316:Tritium; D002794:Choline; D000109:Acetylcholine
Absence de participation de la choline cytoplasmique à la synthèse de l'acétylcholine dans des synaptosomes du striatum de rat.