Selective participation of immunoglobulin V region and major histocompatibility complex products in antigen binding by T cells.
Antigen-binding inhibition studies using microscopic autoradiography were performed on T or B cell-enriched lymphocyte populations. Antibodies specific for the "framework" of immunoglobulin heavy or light chain variable domains (VH or VL), or anti-H-2 and anti-Ia antisera were used. T cell subclasses were separated with anti-Lyt antisera and complement. It was found that antigen-binding T cells of different subclasses can be inhibited selectively with only one of the two anti-V region antibodies. Antigen binding to Lyt-1+ cells was inhibited by anti-VH, while Lyt-2+,3+ cells were inhibited by anti-VL specifically. Anti-Ia antisera inhibited unprimed Lyt-1+ antigen-binding cells, whereas anti-H-2K or anti-H-2D anti-sera inhibited unprimed Lyt-2+,3+ antigen-binding cells, and both classes of immune T antigen-binding cells.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D001666:Binding Sites, Antibody; D006649:Histocompatibility Antigens / Q000276:immunology*; D007106:Immune Sera / Q000494:pharmacology; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region; D008285:Major Histocompatibility Complex; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D013601:T-Lymphocytes / Q000276:immunology*
D006649:Histocompatibility Antigens; D007106:Immune Sera; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region