title: Export of extracellular levansucrase by Bacillus subtilis: inhibition by cerulenin and quinacrine.
abstract: Bacillus subtilis B secretes an inducible, extracellular enzyme, levansucrase. Inhibition studies were undertaken to investigate the possible mechanism of release of this enzyme. The antibiotic cerulenin, at a concentration of 10 micrograms/ml, totally inhibited de novo lipid synthesis in B. subtilis B for at least 1 h, while only slightly reducing protein and RNA synthesis. At this concentration cerulenin, added concomitantly with the inducer sucrose, prevented the release of levansucrase for at least 150 min. This was not due to the prevention of inducer uptake by the cells. The release of the enzyme was also independent of cell division. In B. subtilis 1007 the induction of beta-galactosidase by 5 mM lactose was not prevented by cerulenin. Preliminary evidence indicated the association of a lipid moiety with the enzyme as it passes through the cytoplasmic membrane. Quinacrine (0.2 mM), which inhibits the penicillinase-releasing protease of Bacillus licheniformis, inhibited levansucrase release from B. subtilis B, but had no effect on lipid synthesis.
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis; D002569:Cerulenin / Q000494:pharmacology*; D003864:Depression, Chemical; D005630:Fructans; D006602:Hexosyltransferases / Q000378:metabolism*; D008055:Lipids / Q000096:biosynthesis; D011796:Quinacrine / Q000494:pharmacology*; D012329:RNA, Bacterial / Q000096:biosynthesis; D013395:Sucrose / Q000378:metabolism; D001616:beta-Galactosidase / Q000096:biosynthesis
chemical_list: D000935:Antifungal Agents; D001426:Bacterial Proteins; D005630:Fructans; D008055:Lipids; D012329:RNA, Bacterial; D002569:Cerulenin; D013395:Sucrose; D006602:Hexosyltransferases; C021513:levansucrase; D001616:beta-Galactosidase; D011796:Quinacrine