Quantitative determination of cardiac myosin subunits stained with fast green in SDS-electrophoresis gels.
In the present study a procedure is described which permits simultaneous relative quantification of all myosin subunits in the same fast green stained electrophoresis gel. The relative protein quantification can be performed successively in the same gel using densitometry and the elution method, respectively. Thus, the results obtained with one method can be checked with the other. This procedure provided reliable estimates of the stoichiometry of cardiac myosin subunits within a range of about 20--110 micrograms of total myosin protein per elctrophoresis gel.
D000205:Actomyosin / Q000378:metabolism; D000818:Animals; D003720:Densitometry; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D009206:Myocardium / Q000378:metabolism*; D009218:Myosins / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D013194:Staining and Labeling
D000205:Actomyosin; D009218:Myosins