[Absorption of a proteolytic enzyme originating from plants out of the gastro-intestinal tract into blood and lymph of rats (author's transl)].
The absorption of the proteolytic enzyme bromelain was investigated in adult rats. Bromelain was labelled radioactively with 125-Iodine and intraduodenally applied. During the observation time of 6 hours blood- and lymphsamples were collected and their radioactivity measured. By radiochromatography high molecular protein could be separated from low molecular protein fragments. By means of a rabbit-anti-bromelainserum bromelain could be identified in the agar-double diffusion technique. Results show that adult rats can absorb bromelain up to 40% in a high molecular form. This observation explains the increased proteolytic activity in serum after oral application as well as the clinical effect concerning the treatment of edema or hematoma.
D000818:Animals; D001963:Bromelains / Q000008:administration & dosage / Q000097:blood / Q000378:metabolism*; D005779:Immunodiffusion; D007408:Intestinal Absorption; D007421:Intestine, Small / Q000378:metabolism; D008196:Lymph / Q000201:enzymology; D051381:Rats; D013997:Time Factors
D001963:Bromelains
Die Resorption eines proteolytischen Enzyms pflanzlichen Ursprunges aus dem Magen-Darm-Trakt in das Blut und in die Lymphe von erwachsenen Ratten.