[Stimulation of proliferation of rat spleen cells, in vitro, by a nonspecific acute inflammatory exudate. Modulation of cell response to phytohemagglutinin (PHA)].
The effect of an acute non-specific inflammatory exudate with mitogenic activity on macrophages in culture has been tested on the spontaneous and PHA-induced DNA synthesis of spleen cells in vitro. Stimulatory effect of this exudate was observed on spontaneous DNA synthesis which was detectable over a range of 1 : 4 to 1 : 4,000 concentrations. After optimal PHA stimulation, an inhibition of mitogen-induced DNA synthesis was observed when the cells were exposed to the highest concentrations (up to 1 : 128) of the exudate. Thereafter, the phenomenon could be reversed and the stimulation was maximal at a concentration of 1 : 2,000. When a sub-optimal dose of PHA was used, the simulatory effect was more pronounced and detected from 1 : 8 up to 1 : 4,000 concentrations.
D000208:Acute Disease; D000818:Animals; D002131:Calcium Pyrophosphate; D004247:DNA / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D005122:Exudates and Transudates; D005856:Germ-Free Life; D007249:Inflammation / Q000139:chemically induced; D008934:Mitogens; D010835:Phytohemagglutinins / Q000494:pharmacology*; D051381:Rats; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D013936:Thymidine / Q000378:metabolism
D008934:Mitogens; D010835:Phytohemagglutinins; D004247:DNA; D013936:Thymidine; D002131:Calcium Pyrophosphate
Stimulation de la prolifération des cellules spléniques de Rat in vitro par un exsudat inflammatoire aigu aspécifique. Modulation de la réponse de ces cellules à la phytohémagglutinine (PHA).