Isozyme characterization of cattle (Bos taurus) and American buffalo (Bison bison) cell cultures.
Four Bovidae cell lines (BEK-1, MDBK, Bu and EBTr) were characterized by means of enzymatic biochemical markers. Out of 15 enzymatic systems, 3--adenosine deaminase (Ada), phosphoglucomutase (Pgm) and nucleoside phosphorylase (Np)--were found to be polymorphic and quite suitable for biochemical identification of each cell line. The Bu cell line has shown a Np phenotypic pattern which could be distinctive of the Bison bison species.
D000243:Adenosine Deaminase / Q000235:genetics*; D000818:Animals; D002020:Buffaloes / Q000235:genetics*; D002417:Cattle / Q000235:genetics*; D002478:Cells, Cultured; D004912:Erythrocytes / Q000201:enzymology; D007527:Isoenzymes / Q000235:genetics*; D007668:Kidney / Q000201:enzymology; D007962:Leukocytes / Q000201:enzymology; D009700:Nucleoside Deaminases / Q000235:genetics*; D010430:Pentosyltransferases / Q000235:genetics*; D010641:Phenotype; D010733:Phosphoglucomutase / Q000235:genetics*; D011110:Polymorphism, Genetic; D011683:Purine-Nucleoside Phosphorylase / Q000235:genetics*
D007527:Isoenzymes; D010430:Pentosyltransferases; D011683:Purine-Nucleoside Phosphorylase; D009700:Nucleoside Deaminases; D000243:Adenosine Deaminase; D010733:Phosphoglucomutase