Heterogeneity, immunological comparison and concentration profiles of alpha-fetoproteins derived from late-gestational and early postnatal mouse tissue.
Four distinct alpha-fetoprotein (AFP) species were separated by ion-exchange chromatography of the approximately 70 000 dalton fraction of an extract of late-gestation fetal mice. Although chromatographicaly heterogeneous, antigenic differences among the AFP species resolved were not detected using classical double-diffusion methods. Analysis of the AFP contribution to total body saline-extractable protein of fetal and neonatal mice revealed an abrupt decrease in the fetal AFP contribution to total body saline-extractable protein commencing after the 18th day of gestation.
D000042:Absorption; D000818:Animals; D000831:Animals, Newborn; D000936:Antigen-Antibody Complex; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D000939:Epitopes; D005260:Female; D007106:Immune Sera / Q000494:pharmacology; D005779:Immunodiffusion; D051379:Mice; D008970:Molecular Weight; D011247:Pregnancy; D011506:Proteins; D000509:alpha-Fetoproteins / Q000276:immunology
D000936:Antigen-Antibody Complex; D000939:Epitopes; D007106:Immune Sera; D011506:Proteins; D000509:alpha-Fetoproteins