1H NMR study of the conversion of 13(S)-hydroperoxy linoleic acid by soya bean lipoxygenase I.
The conversion of 13(S)-hydroperoxy linoleic acid by lipoxygenase I at 298 K was monitored by 1H NMR and ultraviolet absorption spectroscopy. The rate constant for the conversion of the hydroperoxide, k = 45.8 +/- 7.5 M-1 . s-1, depends on the concentrations of both enzyme and hydroperoxide. This constant is not affected by O2, nor by solvent isotope effects.
D008041:Linoleic Acids; D008084:Lipoxygenase / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D010545:Peroxides; D010944:Plants / Q000201:enzymology*; D011048:Polarography; D013025:Glycine max
D008041:Linoleic Acids; D010545:Peroxides; D008084:Lipoxygenase