Serum thyrotropin releasing hormone (TRH)-degrading activity: a sensitive and rapid radiochemical assay procedure.
A rapid and sensitive method has been developed to assay thyrotropin releasing hormone (TRH)-degrading activity in serum. In this assay, the formation of proline, a major serum degradation product of TRH (pGlu-His-Pro-NH2), is measured. The procedure is based on the finding that proline can be readily separated from TRH with Dowex 50 by a batchwise procedure in a test tube.
D000818:Animals; D000831:Animals, Newborn; D002852:Chromatography, Ion Exchange / Q000379:methods; D006801:Humans; D007700:Kinetics; D011392:Proline / Q000097:blood; D051381:Rats; D013973:Thyrotropin-Releasing Hormone / Q000097:blood*
D013973:Thyrotropin-Releasing Hormone; D011392:Proline