Cell surface antigens detected on mature and leukemic granulocytic populations by cytotoxicity testing.
Using a microcytotoxicity assay, the serological reactivity of human granulocytes, namely neutrophils and eosinophils, and chronic myeloid leukemia (CML) cells and cultured CML cell lines (K562, NALM-1) were examined. Mature granulocyte forms and cord granulocytes are readily lysed by specific granulocyte cytotoxins that do not react with random T and B lymphocytes, monocytes, red blood cells, or platelets. Furthermore, certain antisera were preferentially cytotoxic for eosinophil-enriched populations. Granulocytotoxin detected antigens on one of three CML blast cell populations tested and K562, but failed to react with NALM-1. By cytotoxicity, mature granulocytes were poor targets for B2-microglobulin and the appropriate HLA antisera although both sera types are absorbed with granulocytes. Furthermore, granulocytes did not possess B-lymphocytes (Ia-like) or blood group A, B, and Rh (D) antigens. Except for K562, both HLA and heterologous B-lymphocyte antisera were cytotoxic for the CML blast cell populations tested.
D000017:ABO Blood-Group System; D000954:Antigens, Surface; D001402:B-Lymphocytes / Q000276:immunology; D002469:Cell Separation; D003601:Cytotoxicity Tests, Immunologic; D003603:Cytotoxins; D004804:Eosinophils / Q000276:immunology; D006098:Granulocytes / Q000276:immunology*; D006680:HLA Antigens; D006801:Humans; D007951:Leukemia, Myeloid / Q000276:immunology*; D012204:Rh-Hr Blood-Group System; D001613:beta 2-Microglobulin
D000017:ABO Blood-Group System; D000954:Antigens, Surface; D003603:Cytotoxins; D006680:HLA Antigens; D012204:Rh-Hr Blood-Group System; D001613:beta 2-Microglobulin