The measurement of antibodies to extractable nuclear antigen.
By the use of a combination of Ouchterlony immunodiffusion and hemagglutination, it was found that 30 of 81 sera (37%) from patients who had systemic lupus erythematosus (SLE) had antibodies to the ribonucleoprotein component of extractable nuclear antigen, and 37 (46%) had antibody to the Sm antigen. Immunodiffusion was more sensitive for detection of anti-ribonucleoprotein, while hemagglutination detected more anti-Sm. Both technics are necessary to define qualitatively the types of antibodies present in SLE sera.
D000974:Antibodies, Antinuclear / Q000032:analysis*; D006386:Hemagglutination Tests; D006801:Humans; D005779:Immunodiffusion; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D009698:Nucleoproteins / Q000276:immunology*; D012261:Ribonucleoproteins / Q000276:immunology*
D000974:Antibodies, Antinuclear; D009698:Nucleoproteins; D012261:Ribonucleoproteins