Reversion of nonsense mutants induced by 4-nitroquinoline-1-oxide in Schizosaccharomyces pombe.
We have studied the reversion of 8 nonsense alleles located in 7 different genes of Schizosaccharomyces pombe using 4-nitroquinoline-1-oxide (NQO) as a mutagenic agent. The nonsense mutants of S. pombe have been classified according to their suppressibility by defined opal and ochre suppressors into a class of efficiently suppressed opal and a class of inefficiency suppressed ochre mutants. The UGA alleles tested all revert consistently with NQO, in agreement with the high specificity of this mutagen for G-residues reported for bacteria and yeast. The UAA alleles show a lack or a low level of reversion with NQO. This low level of reversion is due to the low level of non-G-specific transversions at A sites of the UAA triplet. Within each class of nonsense mutants the extent of induction is site-dependent. We conclude that NQO acts predominantly on G-residues in S. pombe.
D015112:4-Nitroquinoline-1-oxide / Q000494:pharmacology*; D000483:Alleles; D001203:Ascomycota / Q000235:genetics*; D004271:DNA, Fungal / Q000378:metabolism; D006147:Guanine / Q000378:metabolism; D009153:Mutagens; D009154:Mutation / Q000187:drug effects*; D009600:Nitroquinolines / Q000494:pharmacology*; D012568:Schizosaccharomyces / Q000145:classification / Q000235:genetics*
D004271:DNA, Fungal; D009153:Mutagens; D009600:Nitroquinolines; D015112:4-Nitroquinoline-1-oxide; D006147:Guanine