Pig kidney diamine oxidase. A new method of purification.
Diamine oxidase has been purified from pig kidney by a new method to rapidly obtain larger amounts of pure enzyme with a good yield. The enzyme obtained gives only one band in SDS gel electrophoresis. The specific activity and the absorption spectra were identical to those of already preparations homogeneous reported by different methods of purification.
D006631:Amine Oxidase (Copper-Containing) / Q000302:isolation & purification*; D000645:Ammonium Sulfate; D000818:Animals; D011232:Chemical Precipitation; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D006358:Hot Temperature; D007668:Kidney / Q000201:enzymology*; D013053:Spectrophotometry; D013552:Swine
D006631:Amine Oxidase (Copper-Containing); D000645:Ammonium Sulfate