[Formation of the neuromuscular junction in cultures of rat embryonic cells].
The morphological evidence of the primary nerve muscle contacts are described. They consist of areas of cholinesterase activity (detected histochemically) localized on the myotube membranes and of mutiple clusters of ACh receptors whose 125I-alpha-bungarotoxin binding sites are revealed by radio-autography. After the stage of the primary nerve muscle contacts, some of which seem transient, characteristic neuromuscular junctions appear. These neuromuscular junctions which possess subneural infoldings are similar to the end-plates of the Rat in vivo.
D000818:Animals; D002478:Cells, Cultured; D002802:Cholinesterases / Q000378:metabolism; D009024:Morphogenesis; D009469:Neuromuscular Junction / Q000196:embryology* / Q000502:physiology / Q000648:ultrastructure; D051381:Rats; D011950:Receptors, Cholinergic / Q000378:metabolism; D013570:Synaptic Membranes / Q000378:metabolism; D013997:Time Factors
D011950:Receptors, Cholinergic; D002802:Cholinesterases
Formation de jonctions neuromusculaires dans des cultures cellulaires embryonnaires de rat.