[Vital staining of the endothelium of preserved corneae with Trypan Blue (author's transl)].
As a development of Capella's and Kaufman's procedure we examined on four series each with 20 pig corneae the four antifreezing solutions described by the authors singly with regard to their protective effect against freezing. After thawing at +60 degrees within 50 seconds and rehydration with 25% Serum albumin (in the fourth series 10 corneae were rehydrated with polyvinylpyrrolidone) the endothelium was stained with Trypan blue. Microphotographs were made of the endothelial surface, stained nuclei counted and the results were analysed statistically and compared one with other. Hence we could show that with increasing concentration of the antifreeze solution the vitality of the cornea increases.
D000818:Animals; D003315:Cornea / Q000166:cytology / Q000187:drug effects; D003451:Cryoprotective Agents / Q000494:pharmacology; D005615:Freezing; D000882:Haplorhini; D006652:Histological Techniques; D011817:Rabbits; D013552:Swine; D014021:Tissue Preservation; D014022:Tissue Survival; D014343:Trypan Blue
D003451:Cryoprotective Agents; D014343:Trypan Blue
Die Vitalfärbung des Endothels konservierter Hornhäute mit Trypanblau.