Biosynthesis of 6-keto PGF1alpha by microsomal acetone-pentane powder preparations from hog aorta, ram seminal vesicles, and bovine corpora lutea: properties of same.
Acetone-pentane powders of microsomal rich acetone precipitated fractions, have been prepared from hog aortas, ram seminal vesicles, and bovine corpora lutea. These preparations are all active in converting C14 labelled PGH2 to prostacyclin. The reaction was followed by quantitation of the spontaneous hydrolytic product, 6-keto PGF1alpha. The heat stability, pH optima, reactions with inhibitors, and other properties of these types are discussed. The comparative behavior of the respective enzyme preparations shows that while qualitatively they behave in a similar manner, quantitatively, there are significant differences between them, particularly with respect to heat treatment, and response to inhibitors.
D000818:Animals; D001011:Aorta / Q000201:enzymology; D001158:Arteries / Q000201:enzymology*; D001681:Biological Assay; D002417:Cattle; D003338:Corpus Luteum / Q000201:enzymology; D003864:Depression, Chemical; D004789:Enzyme Activation; D011464:Epoprostenol / Q000096:biosynthesis; D005260:Female; D007651:Keto Acids / Q000096:biosynthesis; D008297:Male; D008861:Microsomes / Q000201:enzymology*; D011449:Prostaglandin Endoperoxides / Q000378:metabolism*; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D011460:Prostaglandins F / Q000096:biosynthesis*; D011463:Prostaglandins H / Q000378:metabolism*; D012669:Seminal Vesicles / Q000201:enzymology; D012756:Sheep; D013552:Swine
D007651:Keto Acids; D011449:Prostaglandin Endoperoxides; D011460:Prostaglandins F; D011463:Prostaglandins H; D011464:Epoprostenol; D011451:Prostaglandin-Endoperoxide Synthases