title: Circulating antibodies to peripheral nerve in American trypanosomiasis (Chagas' disease).
abstract: An antibody reacting with Schwann sheaths of myelinated somatic and unmyelinated autonomic peripheral nerve was found in sixty-one out of seventy-one chronic, and nine out of ten acute, Chagas' disease sera. Indirect immunofluorescence (IFL) was carried out on rat, mouse and human somatic nerves and rat sympathetic nerves with initial serum dilutions of 1 : 10, and the staining reached a final titre of 1 : 320 in some cases. The antibodies fixed complement and were absorbed out by lyophilized epimastigotes of T. cruzi. Lipid extraction of the tissue sections enhanced the staining of myelinated nerve, whereas unfixed unmyelinated sympathetic nerve was strongly reactive. Central nervous tissue did not display any positive staining on neurons, glial cells or periaxonal sheaths. Furthermore, by using a double-labelled IFL technique, it was possible to show that a rabbit antiserum raised against guinea-pig spinal cord and the chagasic anti-nerve antibodies reacted with different structures in the rat sciatic nerve. These findings suggest that the reactive antigen(s) could be located on Schwann cells. The majority, but not all, of the chagasic individuals with anti-nerve antibodies also showed the sarcolemmal and endothelial staining (EVI) previously described in Chagas' disease. The possible recognition of Schwann cell antigens by circulating antibodies in Chagas' disease could be relevant, since an autonomic denervation has been postulated as a pathogenic mechanism of cardiomyopathy and megaviscera in this condition.
mesh_terms: D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000954:Antigens, Surface / Q000276:immunology; D001667:Binding, Competitive; D014355:Chagas Disease / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D051379:Mice; D010525:Peripheral Nerves / Q000276:immunology*; D051381:Rats; D012583:Schwann Cells / Q000276:immunology; D012584:Sciatic Nerve / Q000276:immunology; D013564:Sympathetic Nervous System / Q000276:immunology; D014349:Trypanosoma cruzi / Q000276:immunology
chemical_list: D000906:Antibodies; D000954:Antigens, Surface