title: Preferential induction of memory T cells for delayed-type hypersensitivity with reduced and alkylated human serum albumin in mice.
abstract: Reduction and alkylation of human serum albumin (HSA) resulted in molecular aggregation of the protein. The reduced and alkylated antigen (RA-HSA) was lacking in the ability to induce delayed-type hypersensitivity (DTH) response as well as antibody response to native HSA in mice, although native HSA induced both responses. On the other hand, RA-HSA could stimulate a priming function that accelerated and enhanced the DTH response to native HSA, which, however, failed to stimulate the function. Thus, DTH-related memory activity was dissociated from DTH-related effector activity. The DTH-related memory activity, manifested by the accelerated an enhanced response in RA-HSA-primed mice, could be transferred antigen-specifically by their spleen cells and T-cell enriched fraction, but not by their T-cell depleted spleen cells. The RA-HSA-primed T cells, however, failed to transfer the effector function for DTH response. These results suggested that DTH-related memory T cells belong to different subset(s) of T cells from effector T cells for a DTH response.
mesh_terms: D000818:Animals; D000906:Antibodies; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology; D000939:Epitopes; D005260:Female; D006801:Humans; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D007116:Immunization, Passive; D007156:Immunologic Memory; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D011489:Protein Denaturation; D012709:Serum Albumin / Q000276:immunology* / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000906:Antibodies; D000939:Epitopes; D012709:Serum Albumin