Sialic acid metabolism in rats undergoing chemically-induced mammary gland carcinogenesis in specific dietary states.
The enzymes responsible for the activation, transfer and hydrolysis of sialic acids were investigated in female rats with mammary adenocarcinomas induced by administration of a single oral dose (10 mg) of 7,12-dimethylbenz[alpha]anthracene. The carcinogenic process was modulated by the levels and degree of unsaturation of the dietary lipids. Tumor incidence was highest in rats fed a diet containing 20% corn oil, intermediate with 18% coconut oil plus 2% linoleic acid, and lowest in the group receiving a diet with 2% linoleic acid. Sialyltransferase and CMP-N-acetylneuraminic acid synthetase activities were higher in tumors than in control mammary glands. Neuraminidase activity, on the other hand, was higher in control tissue than in tumors. In addition to these tumor-related effects, comparison of the enzyme levels in mammary tissues from control animals of the 3 dietary groups revealed the presence of diet-related effects on sialic acid metabolism. In the livers of tumor-bearing rats, only minor changes of enzyme activities were detected.
D015127:9,10-Dimethyl-1,2-benzanthracene; D000230:Adenocarcinoma / Q000209:etiology / Q000378:metabolism; D000818:Animals; D001551:Benz(a)Anthracenes; D004041:Dietary Fats / Q000009:adverse effects*; D005260:Female; D008099:Liver / Q000378:metabolism; D008325:Mammary Neoplasms, Experimental / Q000209:etiology / Q000378:metabolism*; D000216:N-Acylneuraminate Cytidylyltransferase / Q000378:metabolism; D009439:Neuraminidase / Q000378:metabolism; D051381:Rats; D012794:Sialic Acids / Q000378:metabolism*; D012799:Sialyltransferases / Q000378:metabolism
D001551:Benz(a)Anthracenes; D004041:Dietary Fats; D012794:Sialic Acids; D015127:9,10-Dimethyl-1,2-benzanthracene; D012799:Sialyltransferases; D000216:N-Acylneuraminate Cytidylyltransferase; D009439:Neuraminidase