title: Lysozyme: primary bactericidin in human plasma serum active against Bacillus subtilis.
abstract: The in vitro bactericidal reaction of human plasma serum against Bacillus subtilis was investigated. Human lysozyme was purified to homogeneity, and antiserum was prepared against the enzyme. The anti-lysozyme immunoglobulin G was used as a specific inhibitor in bactericidal and bacteriolytic reactions. It was found that at low serum concentrations lysozyme was the primary bactericide active against B. subtilis. At appreciably higher serum concentrations, a lysozyme-independent bactericidal activity was also demonstrated.
mesh_terms: D000906:Antibodies; D001412:Bacillus subtilis / Q000276:immunology*; D001433:Bacteriolysis; D001770:Blood Bactericidal Activity; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation; D006358:Hot Temperature; D006801:Humans; D007938:Leukemia / Q000652:urine; D009113:Muramidase / Q000097:blood* / Q000276:immunology / Q000302:isolation & purification
chemical_list: D000906:Antibodies; D009113:Muramidase