[In vitro study of glucuronoconjugation of the toxin from Penicillium roqueforti (P.R.T.) and its metabolites].
Rat liver microsome UDP-glucuronyltransferase and labelled UDP-glucuronic acid were incubated either with P.R.T. or the compounds obtained by the in vitro metabolism of the toxin. Under the same conditions, labelled P.R.T. or its labelled metabolites were incubated with UDP glucuronyltransferase. Radioactive metabolites were produced with Eremofortin C and Eremofortin C alcohol and in each case, were identified as the corresponding beta-glucuronide conjugate. No measurable glucuronidation of P.R.T. or P.R.T. alcohol was observed. The results outlined in this paper show a good correlation between the biological effects and the ability of forming a glucuronide conjugate.
D000818:Animals; D005965:Glucuronates / Q000378:metabolism*; D014453:Glucuronosyltransferase / Q000378:metabolism; D066298:In Vitro Techniques; D008862:Microsomes, Liver / Q000201:enzymology; D009183:Mycotoxins / Q000378:metabolism*; D010407:Penicillium / Q000378:metabolism*; D051381:Rats
D005965:Glucuronates; D009183:Mycotoxins; D014453:Glucuronosyltransferase
Etude in vitro de la glucuronoconjugaison de la toxine de Penicillium roqueforti (P.R.T.) et de ses métabolites associés.