DNA synthesis in toluene-treated bacteriophage-infected minicells or Bacillus subtilis.
Bateriophage (phi29, SPP1, or SPO1)-infected, toluene-treated minicells of Bacillus subtilis are capable of limited amounts of non-replicative DNA synthesis as measured by incorporation of [3H]dTTP into a trichloroacetic acid-precipitable form. The [3H]dTTP is covalently incorporated into small DNA fragments which result from the degradation of a small percentage of the infecting phage genomes (molecular weights in the range of 2 . 10(5)). Short exposure of the DNA molecules containing the incorporated [3H]dTMP to Escherichia coli exonuclease III results in over 90% of the E13H]dTMP being converted to a trichloroacetic acid-soluble form. The synthesis is totally dependent on host-cell enzymes and is not inhibited by the addition of chloramphenicol, rifampicin, nalidixic acid and mitomycin C and only slightly (approx. 20%) inhibited by the addition of 6-(p-hydroxyphenylazo)-uracil.
D001412:Bacillus subtilis / Q000187:drug effects / Q000378:metabolism*; D001435:Bacteriophages / Q000378:metabolism*; D004269:DNA, Bacterial / Q000096:biosynthesis*; D003854:Deoxyribonucleotides / Q000378:metabolism; D013942:Thymine Nucleotides / Q000378:metabolism; D014050:Toluene / Q000494:pharmacology*
D004269:DNA, Bacterial; D003854:Deoxyribonucleotides; D013942:Thymine Nucleotides; D014050:Toluene