[Preparation of human platelets without the use of anticoagulants and study of the effect of Catt on aggregation].
A method for the preparation of human blood platelets is presented which substitutes the use of anticoagulants by a gelfiltration for the removal of plasma calcium from native blood. In a second step the platelets are separated from the gel filtered blood by a centrifugation on a Ficoll density gradient. The anticoagulant-free platelets reveal an intact morphological feature and a normal aggregation behaviour in response to different aggregation inducers. It was found that in ADP-induced aggregation monophasic aggregation is shifted to a biphasic one by increasing concentrations of extracellular calcium.
D001792:Blood Platelets / Q000648:ultrastructure; D001798:Blood Proteins / Q000302:isolation & purification; D002118:Calcium / Q000502:physiology*; D002469:Cell Separation / Q000379:methods*; D002850:Chromatography, Gel; D006801:Humans; D008055:Lipids / Q000302:isolation & purification; D010974:Platelet Aggregation
D001798:Blood Proteins; D008055:Lipids; D002118:Calcium
Präparation menschlicher Blutplättchen ohne Verwendung von Antikoagulantien und Untersuchung des Ca++-Einflusses auf die Aggregation.