Photoelectric properties and detection of the aromatic carcinogens benza[a]pyrene and dimethylbenzanthracene.
The absolute photoelectron quantum yield spectra for benzo[a]pyrene and dimethylbenzanthracene are presented in the wavelength range 180--230 nm. These polycyclic aromatic carcinogens have photoelectron quantum yields of approximately 2 x 10(-3) electrons per incident photon at 180 nm. The quantum yields fall off quickly and monotonically at wavelengths longer than 210 nm (5.9 eV). Threshold values for benzo[a]pyrene and dimethylbenzanthracene are 5.25 +/- 0.06 cV and 5.27 +/- 0.04 eV, respectively. The photoelectron quantum yields of benzo[a]pyrene and dimethylbenzanthracene are several orders of magnitude greater than typical components of biological membranes (amino acids, phospholipids, and polysaccharides). Preliminary micrographs of benzo[a]pyrene and dimethylbenzanthracene sublimed onto poly(L-lysine) and onto dimyristoyl phosphatidylcholine demonstrate the high contrast of small crystallites of carcinogens against a background of membrane components. These results and calculations involving relative contrast factors suggest that the distribution of these carcinogens in biological membranes can be determined by using photoelectron microscopy.
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000032:analysis*; D001551:Benz(a)Anthracenes / Q000032:analysis*; D001580:Benzopyrenes / Q000032:analysis*; D004560:Electricity; D008854:Microscopy, Electron / Q000379:methods; D010777:Photochemistry / Q000379:methods
D001551:Benz(a)Anthracenes; D001580:Benzopyrenes; D015127:9,10-Dimethyl-1,2-benzanthracene