Effect of aluminium chloride on binding of 4-hydroxyamino-quinoline 1-oxide to nucleotides.
Effect of aluminium chloride on the binding of carcinogenic 4-hydroxyaminoquinoline 1-oxide (4-HAQO) with mouse lung DNA, RNA, and various homopolyribonucleotides was examined in vitro, in the presence of seryl-AMP. Mouse lung DNA, RNA, or homopolyribonucleotide [poly(A), poly(G), poly(I), poly(X), poly(C), or poly(U)] was pretreated with aluminium chloride in an ice bath and the binding with 4-HAQO was examined. Binding with DNA, RNA, poly(A), and poly(G) was markedly inhibited, and their binding rates were 46%, 56%, 53%, and 18% of that of the control, respectively. Binding with poly(C) and poly(U) was hardly different from that of the control. Consequently, effect of aluminium chloride in inhibiting the binding of 4-HAQO with mouse lung DNA and RNA is assumed to be due to the inhibition of its binding with guanine. Effect of various metals (Mg2+, Mn2+, Co2+, Ni2+, Cu2+, Zn2+, and Fe3+) on the binding of 4-HAQO with mouse lung DNA was examined and it was found that aluminium chloride had the strongest inhibitory effect, followed by copper and zinc. Trivalent iron showed hardly any inhibition.
D015108:4-Hydroxyaminoquinoline-1-oxide / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000535:Aluminum / Q000494:pharmacology*; D000634:Aminoquinolines / Q000378:metabolism*; D000818:Animals; D002413:Cations, Divalent / Q000494:pharmacology; D002712:Chlorides / Q000494:pharmacology; D004247:DNA / Q000378:metabolism; D005290:Ferric Compounds / Q000494:pharmacology; D006851:Hydrochloric Acid; D006868:Hydrolysis; D066298:In Vitro Techniques; D008168:Lung / Q000378:metabolism; D008297:Male; D051379:Mice; D009711:Nucleotides / Q000378:metabolism*; D012313:RNA / Q000378:metabolism
D000634:Aminoquinolines; D002413:Cations, Divalent; D002712:Chlorides; D005290:Ferric Compounds; D009711:Nucleotides; D015108:4-Hydroxyaminoquinoline-1-oxide; D012313:RNA; D004247:DNA; D000535:Aluminum; D006851:Hydrochloric Acid