[Effect of lysozyme, ethylenediaminetetraacetate, magnesium and mannittol on spheroplast formation in Anacystis nidulans].
Three periods (the lag period and periods of intensive and decelerated spheroplast formation) can be detected in the action of lysozyme on the cells of Anacystis nidulans; this seems to be due to peculiarities in the cell wall structure of the cyanobacterium and heterogeneity of the culture. EDTA at concentrations of 0.175--0.7 mM has effect on the duration of the lag period but increases the rate of spheroplast formation during the subsequent periods. However, the action of EDTA in complex with lysozyme for 3 hours causes lysis of some of the spheroplasts being formed. The concentration of mannitol below 0.4 M decreases the yield of spheroplasts. The level of intact spheroplasts prepared from A. nidulans suggests that at least part of them is capable of reversing into whole cells.
D000458:Cyanobacteria / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004492:Edetic Acid / Q000494:pharmacology*; D008274:Magnesium / Q000494:pharmacology*; D008353:Mannitol / Q000494:pharmacology*; D009113:Muramidase / Q000494:pharmacology*; D013104:Spheroplasts / Q000187:drug effects* / Q000302:isolation & purification
D008353:Mannitol; D004492:Edetic Acid; D009113:Muramidase; D008274:Magnesium
Vliianie lizotsima, étilendiaminotetraatsetata, magniia i mannita na obrazovanie sferoplastov u Anacystis nidulans.