Mycolic acids. A reinvestigation.
Mycolic acids derived from the cell walls of Mycobacterium bovis BCG, Mycobacterium bovis Bovinus I, Mycobacterium smegmatis, and Mycobacterium tuberculosis H37Rv have been fractionated as their p-bromophenacyl esters by a two-step high performance liquid chromatographic procedure: 1) adsorption chromatography on 10-micrometer particle size silica gel, and 2) reverse phase partition chromatography on a 10-micrometer particle size support containing a C18 bonded phase. This procedure has resulted in the isolation of approximately 24 mycolic acids from each bacterium (very likely homologs of various mycolate types) instead of the two to four that have previously been described. The implication of these results on the previously determined structures of these fatty acids is discussed.
D008970:Molecular Weight; D009161:Mycobacterium / Q000032:analysis*; D009163:Mycobacterium bovis / Q000032:analysis; D009169:Mycobacterium tuberculosis / Q000032:analysis; D009171:Mycolic Acids / Q000302:isolation & purification; D013045:Species Specificity
D009171:Mycolic Acids