Transfomration of arachidonic acid into 12-hydroxy-5,8,10,14-eicosatetraenoic acid by mouse peritoneal macrophages.
Mouse peritoneal macrophages were incubated at 37 degrees C for 30 min with arachidonic acid (all-cis-5,8,11,14-eicosatetraenoic acid). Oxygenation of arachidonic acid in mouse peritoneal macrophages occurs by two major pathways: fatty acid cyclooxygenase and lipoxygenase. The major metabolite of the latter is 12-hydroxy-5,8,10,14-eicosatetraenoic acid which was identified by gas liquid chromatography on high resolution glass capillary column and mass spectrometry.
D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism*; D001202:Ascitic Fluid / Q000166:cytology; D002849:Chromatography, Gas; D008084:Lipoxygenase / Q000378:metabolism*; D008264:Macrophages / Q000378:metabolism*; D013058:Mass Spectrometry; D051379:Mice
D001095:Arachidonic Acids; D008084:Lipoxygenase