[Biosynthesis of cellulolytic enzymes and xylanase during submerged cultivation of the fungus Aspergillus terreus 17P].
The fungus Aspergillus terreus 17P--producer of cellulolytic enzymes--was cultivated in the Biotec 10 l fermenter on the medium containing minced and heated (at 200 degrees) wheat straw aerated with a different rate. At the mixing rate of 350 rpm and aeration rate of 0.7 r/rpm on the fourth day the culture liquid was obtained whose filtrate contained an active complex of cellulolytic enzymes and xylanase: CI--3.4; APB--1.1, Cx--35.7, cellobiase--0.23, xylanase--73.8 units/ml. The fractionation of the culture liquid filtrate with ammonium sulphate showed that the fraction precipitated at an interval of saturation of 0.3--0.7 contained the largest portion of cellulolytic enzymes and xylanase. The isolated enzymic preparations had a cellulolytic and xylanase activity and contained lipase, pectinase, laminarinase. They also contained low quantities of amylase, protease, beta-1,4- and beta-1,6-glucanase. Enzymic hylrolysis by the Asp. terreus 17P preparation of straw yielded glucose and xylose, of cotton, Na-KMC, cellobiose--glucose, Xylane hydrolyzate contained xylose and arabinose.
D000681:Amylases / Q000302:isolation & purification; D001230:Aspergillus / Q000201:enzymology*; D004693:Glucan Endo-1,3-beta-D-Glucosidase / Q000302:isolation & purification; D006026:Glycoside Hydrolases / Q000096:biosynthesis*; D008049:Lipase / Q000302:isolation & purification; D010447:Peptide Hydrolases / Q000302:isolation & purification; D011096:Polygalacturonase / Q000302:isolation & purification; D014990:Xylans; D001617:beta-Glucosidase / Q000096:biosynthesis
D014990:Xylans; D008049:Lipase; D000681:Amylases; D006026:Glycoside Hydrolases; D011096:Polygalacturonase; D001617:beta-Glucosidase; D004693:Glucan Endo-1,3-beta-D-Glucosidase; D010447:Peptide Hydrolases
Biosintez tselliuloliticheskikh fermentov i ksilanazy v usloviiakh glubinnogo Aspergillus terreus 17P.