Comparison of the effects of beeswax: trioctanoin and trioctanoin vehicles on 3-methylcholanthrene, benzo[a]pyrene, and 7,12-dimethylbenz[a]anthracene subcutaneous carcinogenesis in three strains of mice and one hybrid.
Subcutaneous tumor induction with three dose levels of 3-methylcholanthrene (MCA), benzo[a]pyrene (BP), and 7,12-dimethylbenz[a]anthracene (DMBA) in two vehicles was studied in C3H/Anf Cum, C57BL/6 Cum, DBA/2J, and (C57BLXC3H/Anf)F1 (BC3F1/Cum) mice. Median tumor dose levels were significantly lower when the three carcinogens were suspended in trioctanoin. When beeswax: trioctanoin (B:T) was used as a vehicle, the three carcinogens differed in their abilities to be absorbed or solubilized from the vehicle by the three strains of mice and the hybrid. In C3H/Anf mice, BP in B:T failed to produce tumors. In BC3F1 mice, no tumors were produced by MCA, BP, or DMBA in B:T. In C57BL/6 mice, no tumors were produced with DMBA or MCA in B:T. In DBA/2 mice, no tumors were produced by BP or MCA in B:T. These results indicated that the interpretation of tumor induction results obtained with B:T vehicle may be related to the conditions of bioassay rather than to the carcinogenic potential of a compound.
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000008:administration & dosage*; D000818:Animals; D001516:Bees; D001551:Benz(a)Anthracenes / Q000008:administration & dosage*; D001580:Benzopyrenes / Q000008:administration & dosage*; D002210:Caprylates / Q000008:administration & dosage*; D005260:Female; D007279:Injections, Subcutaneous; D008748:Methylcholanthrene / Q000008:administration & dosage*; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D009374:Neoplasms, Experimental / Q000139:chemically induced; D012983:Soft Tissue Neoplasms / Q000139:chemically induced*; D013045:Species Specificity; D014280:Triglycerides / Q000008:administration & dosage*; D014885:Waxes / Q000008:administration & dosage*
D001551:Benz(a)Anthracenes; D001580:Benzopyrenes; D002210:Caprylates; D014280:Triglycerides; D014885:Waxes; D008748:Methylcholanthrene; D015127:9,10-Dimethyl-1,2-benzanthracene; C003637:tricaprylin