Isolation of glucosyltransferase from Streptomyces aureofaciens.
Streptomyces aureofaciens B 96 grown on a synthetic medium glucosylated exogenous 1,2-dihydroxy-9,10-anthraquinone (alizarin). The glucosylation was inhibited by 2,4-dinitrophenol added to the cultivation medium. A cell-free preparation was obtained from the mycelium isolated after 16 h of growth and was found to catalyze the transfer of glucose from glucosyluridyl diphosphate to 1,2-dihydroxy-9,10-anthraquinone, giving rise to 1-hydroxy-2-(beta-D-glucopyranosyloxy)-9,10-anthraquinone.
D000880:Anthraquinones / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis; D002474:Cell-Free System; D004140:Dinitrophenols / Q000494:pharmacology; D005960:Glucosides / Q000096:biosynthesis; D005964:Glucosyltransferases / Q000302:isolation & purification* / Q000378:metabolism; D013304:Streptomyces aureofaciens / Q000201:enzymology*; D013307:Streptomycin / Q000494:pharmacology; D013395:Sucrose / Q000378:metabolism
D000880:Anthraquinones; D001426:Bacterial Proteins; D004140:Dinitrophenols; D005960:Glucosides; D013395:Sucrose; D005964:Glucosyltransferases; D013307:Streptomycin