[Effect of temperature on RNA synthesis in Escherichia coli CRT 266 (dna Bts) following infection with bacteriophage T3].
Infection of the temperature-sensitive E. coli CRT 266 (dnaBts) with T3-phages at the temperature of 30 degrees C and 35 degrees C, respectively, induced T3-specific RNA synthesis with a maximum rate at 7 min (30 degrees C) and 4.5 min (35 degrees C) after infection. At temperatures above 40 degrees C no T3-induced RNA synthesis could be observed. Infection of E. coli CR 34--45 (dnaB+) with T3 phages at 30 degrees C, 35 degrees C and at temperatures above 40 degrees C, however, produced T3-specific RNA synthesis. The maximum of T3-induced RNA synthesis could be observed between 7 min and 3 min depending on the temperature during infection. The inability to form T3-specific RNA after infection of E. coli CRT 266 at nonpermissive temperatures may be a cause for the absence of the formation of T3 phages and lysis of the host cells.
D002701:Chloramphenicol / Q000494:pharmacology; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D012329:RNA, Bacterial / Q000096:biosynthesis*; D012367:RNA, Viral / Q000096:biosynthesis; D012293:Rifampin / Q000494:pharmacology; D013604:T-Phages / Q000254:growth & development* / Q000378:metabolism; D013696:Temperature
D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012367:RNA, Viral; D002701:Chloramphenicol; D012293:Rifampin
Einfluss der Temperatur auf die RNA-Synthese von Escherichia coli CRT 266 (dnaBts) nach Infektion mit dem Bakteriophagen T3.