Optimal conditions for Fc receptor-ligand interaction.
The temperature and time dependence for Fc receptor-ligand interaction have been investigated in a murine lymphoblastoid cell line. The binding of antibody-coated erythrocytes or radioiodinated heat-aggregated IgG was evaluated at varying time points during incubation at 4, 21 or 37 degrees C. Maximum rosette formation was observed by 10 min incubation at 21 degrees C if cell suspensions were first centrifuged at 200 g for 2 min. Longer incubation periods were required in the absence of centrifugation. The kinetics of aggregate IgG binding were similar to rosette formation without centrifugation. The results demonstrate that receptor-ligand interaction in this system is both temperature and time-dependent.
D000906:Antibodies; D004347:Drug Interactions; D004912:Erythrocytes; D007141:Immunoglobulin Fc Fragments / Q000378:metabolism*; D007142:Immunoglobulin gamma-Chains; D008024:Ligands; D011955:Receptors, Drug; D012397:Rosette Formation; D013696:Temperature; D013997:Time Factors
D000906:Antibodies; D007141:Immunoglobulin Fc Fragments; D007142:Immunoglobulin gamma-Chains; D008024:Ligands; D011955:Receptors, Drug