Characterization of a mouse DNA clone containing an immunoglobulin variable region gene.
A 4.8 kilobase mouse embryo DNA fragment was inserted into a phage lambda genome and was subsequently characterized by electron microscopy, restriction enzyme mapping and partial DNA sequencing. This fragment contains a 400 base sequence which is homologous to that of an immunoglobulin light lambda chain mRNA which spans 3.3 to 3.7 kilobases from one end of the fragment. Restriction enzyme mapping as well as partial nucleotide sequencing of the 3' terminal of the homology region confirm the previous conclusion [Tonegawa, Brack, Hozumi and Schuller, Proc. Natl. Acad. Sci. USA. 74, 3518-3522 (1977)] that the cloned DNA fragment contains a Vlambda gene sequence which is separate from any Clambda sequence.
D000818:Animals; D000917:Antibody Formation; D001483:Base Sequence; D002460:Cell Line; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000096:biosynthesis; D004622:Embryo, Mammalian; D007143:Immunoglobulin Heavy Chains / Q000096:biosynthesis*; D007142:Immunoglobulin gamma-Chains / Q000096:biosynthesis*; D051379:Mice; D008854:Microscopy, Electron; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000096:biosynthesis
D004274:DNA, Recombinant; D007143:Immunoglobulin Heavy Chains; D007142:Immunoglobulin gamma-Chains; D012333:RNA, Messenger; D004262:DNA Restriction Enzymes