Molecular cloning of the gene region coding for the chloroplast rRNA of Euglena gracilis.
E. gracilis chloroplast DNA Bam fragments E and D, coding for rRNA were cloned separately using the plasmid pBR 322 as vector and E. coli as host. The newly constructed recombinant plasmids EgcKS 8 and EgcKS 11 (containing the Bam HI fragments E and D respectively) were analysed and characterized by gel electrophoresis, electronmicroscopy and analytical ultracentrifugation.
D000818:Animals; D002736:Chloroplasts; D004269:DNA, Bacterial / Q000235:genetics; D004274:DNA, Recombinant / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D005056:Euglena gracilis / Q000235:genetics*; D005796:Genes; D010957:Plasmids; D012335:RNA, Ribosomal / Q000235:genetics*
D004269:DNA, Bacterial; D004274:DNA, Recombinant; D012335:RNA, Ribosomal