Light microscopic identification and photography of Golgi impregnated central nervous system neurons during sectioning for electron microscopy.
A method is described for a rapid and systematic light microscopic documentation of Golgi impregnated neurons while they are being sectioned for electron microscopy. A drawing under the light microscope of a Golgi impregnated neuron is made first; subsequently thin sections of the tissue containing this neuron are cut in the same plane as for light microscopy. During thin sectioning the chuck containing the block is taken out of the ultramicrotome at regular intervals and placed in a special device under a light microscope. The neuron is photographed to record the stage of sectioning. Comparison of the micrographs indicates which part of the neuron and its dendritic tree are contained in the thin sections. No semithin sections are used and therefore no material is lost for reconstruction.
D000818:Animals; D002540:Cerebral Cortex / Q000648:ultrastructure*; D003584:Cytological Techniques; D008116:Lizards; D008854:Microscopy, Electron; D008867:Microtomy; D009474:Neurons / Q000648:ultrastructure*; D010784:Photomicrography; D013194:Staining and Labeling