title: Outer membrane protein antigens in an enzyme-linked immunosorbent assay for Salmonella enteric fever and meningococcal meningitis.
abstract: Outer membrane protein preparations were obtained from strains of Salmonella and Neisseria meningitidis. Solubilized cell envelope (CE) fractions from S. typhi and Salmonella groups A, B, C, and E had very similar electrophoretic mobilities on polyacrylamide gel, and common antigens were demonstrated by immunodiffusion. CE appeared to be a more satisfactory antigen than the more purified preparation (T/TEI) in the enzyme-linked immunosorbent assay (ELISA) with sera from typhoid and paratyphoid patients. With either antigen, however, the presence of antibodies was demonstrated in acute- and vonvalescent-phase sera. In the case of N. meningitidis infections, the crude (STA) and the more purified antigens (T/TEI) were equally satisfactory, and a rise in antibody titer could easily be demonstrated with paired acute- and convalescent-phase sera. The ELISA appears to be a simple but highly sensitive test for the detection of antibodies by using outer membrane protein antigens.
mesh_terms: D000942:Antigens, Bacterial / Q000032:analysis; D001426:Bacterial Proteins / Q000276:immunology*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D005779:Immunodiffusion; D007124:Immunoenzyme Techniques / Q000379:methods*; D008565:Membrane Proteins / Q000276:immunology*; D008585:Meningitis, Meningococcal / Q000175:diagnosis*; D010284:Paratyphoid Fever / Q000175:diagnosis; D014390:Tuberculosis, Meningeal / Q000175:diagnosis; D014435:Typhoid Fever / Q000175:diagnosis*
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D008565:Membrane Proteins