Enzyme-linked immunosorbent choriomammotropin assay.
We have devloped an enzyme-linked immunosorbent assay for determining choriomammotropin (human placental lactogen) in serum. Unlabeled hormone competes with choriomammotropin-beta-galactosidase conjugate for antibody bound to polystyrene tubes. The entire assay can be performed in 2.5 h with good precision. The coefficient of variation for one sample with a mean concentration of 5.6 mg/L, assayed 10 times on the same day, was 5.7%. The coefficient of variation for nine samples (3.5 to 9.0 mg/L) assayed on five different days was 7.9%. Forty-eight clinical samples were assayed (y) and compared with results obtained by radial immunodiffusion (x). The resulting regression equation was: y = 1.05x + 0.78; r = 0.91.
D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007202:Indicators and Reagents; D008301:Maleimides; D010928:Placental Lactogen / Q000097:blood*; D013388:Succinimides; D001616:beta-Galactosidase
D007202:Indicators and Reagents; D008301:Maleimides; D013388:Succinimides; D010928:Placental Lactogen; D001616:beta-Galactosidase