Differential biosynthesis of polyunsaturated fatty acids by Tetrahymena supplemented with ergosterol.
Tetrahymena grown with foreign sterols such as ergosterol incorporate them into cellular membranes at the expense of the native compound, tetrahymanol. It is shown that cells grown with ergosterol have a lessened capacity to produce the polyunsaturated linoleic and gamma-linolenic acids from [14C]oleic acid. However, the same cells have normal capacities to introduce double bonds at C-6 into linoleate, alpha-linolenate, or cis-vaccenate. Thus, a presumed 12-desaturase is inhibited in the presence of ergosterol, while desaturation at C-6 is unaffected.
D004875:Ergosterol / Q000378:metabolism / Q000494:pharmacology; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis*; D008041:Linoleic Acids / Q000096:biosynthesis; D008042:Linolenic Acids / Q000096:biosynthesis; D013769:Tetrahymena pyriformis / Q000187:drug effects / Q000378:metabolism*
D005231:Fatty Acids, Unsaturated; D008041:Linoleic Acids; D008042:Linolenic Acids; D004875:Ergosterol