Effects of the hepatic S9 fraction from aroclor-1254-treated rats on the mutagenicity of benzo[alpha]pyrene and 2-aminoanthracene in the Salmonella/microsome assay.
The mutagenicity of benzo[alpha]pyrene and 2-aminoanthracene for Salmonella typhimurium TA98 in the plate-incorporation test was studied using liver S9 from untreated and aroclor-1254-treated rats. The induction of liver S9 protein, arylhydrocarbon hydroxylase (AHH), and cytochrome P448/450 was followed with time. There was no change in protein concentrations with induction; AHH and cytochrome levels were increased at 1, 3, 5 and 7 days post Aroclor treatment. Benzo[alpha]pyrene mutagenicity was enhanced with Aroclor treatment while 2-aminoanthracene mutagenicity was depressed. The benzo[alpha]pyrene mutagenicity showed a positive correlation with the levels of AHH and cytochrome on the plate; 2-aminoanthracene showed a negative correlation with activity in induced samples.
D000818:Animals; D000873:Anthracenes / Q000494:pharmacology*; D001140:Aroclors / Q000494:pharmacology*; D001580:Benzopyrenes / Q000494:pharmacology*; D005821:Genetic Techniques; D008099:Liver / Q000378:metabolism*; D009153:Mutagens / Q000378:metabolism*; D011078:Polychlorinated Biphenyls / Q000494:pharmacology*; D051381:Rats; D012486:Salmonella typhimurium / Q000235:genetics
D000873:Anthracenes; D001140:Aroclors; D001580:Benzopyrenes; D009153:Mutagens; D011078:Polychlorinated Biphenyls