Effect of modification on physico-chemical and biological properties of haptoglobin. Reaction with N-bromusuccinimide and 2-hydroxy-5-nitrobenzyl bromide.
N-Bromosuccinimide and 2-hydroxy-5-nitrobenzyl bromide have been used for modification of tryptophan residues in human haptoglobin (Hp) type 2-1. Modification of three exposed tryptophan residues reduced considerably both the Hp-haemoglobin interaction and binding of the antibody against the native protein. Modification of the remaining 7-8 tryptophan residues resulted in a complete loss of those properties. Antisera directed against Hp with the modified tryptophan residues appeared to be highly specific in immunological reactions.
D015077:2-Hydroxy-5-nitrobenzyl Bromide; D001665:Binding Sites; D001974:Bromosuccinimide; D055598:Chemical Phenomena; D002621:Chemistry; D006242:Haptoglobins / Q000276:immunology / Q000378:metabolism*; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D009596:Nitrophenols; D011485:Protein Binding; D014364:Tryptophan
D006242:Haptoglobins; D006454:Hemoglobins; D009596:Nitrophenols; D015077:2-Hydroxy-5-nitrobenzyl Bromide; D014364:Tryptophan; D001974:Bromosuccinimide