Purification of antibodies to influenza A virus structural proteins by affinity chromatography, and their participation in haemagglutination-inhibition, neutralization and enzyme-linked immunosorbent assay.
Affinity chromatography was used to purify rabbit antibodies to common and strain-specific antigeneic determinants of haemagglutinin, to neuraminidase, and to a combination of the internal proteins of influenza A viruses. The purity of the antibodies was assessed by haemagglutination-inhibition, enzyme-linked immunosorbent assay (ELISA) and competition ELISA. The antibodies were examined for their participation in neutralization and haemagglutination-inhibition assays, and in ELISA. ELISA was found to be the most sensitive technique for detecting antibodies. Antibodies to the common and strain-specific determinants of haemagglutinin were shown to participate in neutralization assay and ELISA, but only those antibodies to the common determinants were detected by haemagglutination-inhibition.
D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D006385:Hemagglutination Inhibition Tests; D009980:Influenza A virus / Q000276:immunology*; D009500:Neutralization Tests; D011817:Rabbits; D014764:Viral Proteins / Q000276:immunology*
D000914:Antibodies, Viral; D000939:Epitopes; D014764:Viral Proteins