Exclusion of VHa and VHy loci expression on individual B cells from normal and VH allotype-suppressed rabbits.
The distribution of two heavy chain subgroups, VHa and VHy, on rabbit peripheral blood lymphocytes was examined by double membrane immunofluorescence. Fluroescent anti-a1 and anti-y33 were found to react with separate B cell populations; no doubly stained cells were observed. Further evidence for the independent expression of genes controlling the VHa and VHy subgroups were obtained by neonatal suppression of a 2 or y33 in a2y33/a3y- heterozygous rabbits. Suppression of VHa did not affect the expression of VHy, nor did the suppression of VHy affect the expression of VHa. The expression of a single VH gene per B cell is in marked contrast to the simultaneous expression of multiple CH genes.
D000375:Aging; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D002874:Chromosome Mapping; D005260:Female; D005455:Fluorescent Antibody Technique; D007126:Immunoglobulin Allotypes; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007135:Immunoglobulin Variable Region; D007165:Immunosuppression Therapy; D008214:Lymphocytes / Q000276:immunology; D011817:Rabbits
D007126:Immunoglobulin Allotypes; D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region