[Respiration-stimulated activation of DNAase I associated with mitochondria].
The influence of respiration and Ca++ transport in the liver mitochondria on the activation of DNAase I, associated with these organelles, was studied. It was shown that 96% of the total activity of this enzyme in mitochondria is in the latent state. Aeration of the mitochondrial suspension leads to a sharp increase in the enzyme activity. The activation of DNAase I is inhibited by EGTA addition (optimal pH 8.0), and stimulated in mitochondria, releasing Ca++. It is concluded that the activation of DNAase I is dependent on the state of cellular energetics. Participation of mitochondrial phospholypase A, activated by the Ca++ release from mitochondria during DNAase I activation is suggested.
D000332:Aerobiosis; D000818:Animals; D001693:Biological Transport, Active; D002118:Calcium / Q000378:metabolism*; D003851:Deoxyribonucleases / Q000378:metabolism*; D004533:Egtazic Acid / Q000494:pharmacology; D007700:Kinetics; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D010101:Oxygen Consumption / Q000187:drug effects; D051381:Rats
D004533:Egtazic Acid; D003851:Deoxyribonucleases; D002118:Calcium
Stimulireumaia bykhaniem aktivatsiia assotsiirovannoi s mitokhondriiami DNK-Azy I.