A solid-phase radioimmunoassay for bacterial lipopolysaccharide.
A radioimmunoassay for E. coli 055:B5 lipopolysaccharide (LPS) is described. The LPS was derivatised by two new methods and subsequently radiolabeled with 125I to a specific activity of 2-4 mCi/mg without apparent loss in its biophysical, immunological or biological activities. Using antibody-coated polystyrene tubes, a solid-phase radioimmunoassay was developed with a sensitivity of 10-500 ng/ml of LPS.
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis; D002850:Chromatography, Gel; D004926:Escherichia coli / Q000276:immunology; D005779:Immunodiffusion; D007928:Lethal Dose 50; D008070:Lipopolysaccharides / Q000276:immunology*; D008213:Lymphocyte Activation; D051379:Mice; D010648:Phenylacetates; D011817:Rabbits; D011863:Radioimmunoassay; D014439:Tyramine
D000907:Antibodies, Bacterial; D008070:Lipopolysaccharides; D010648:Phenylacetates; D014439:Tyramine