Rescue of immunoglobulin secretion from human neoplastic lymphoid cells by somatic cell hybridization.
B leukemia cells from four different patients were hybridized with a mouse myeloma cell line with polyethylene glycol as a fusing agent. The original leukemia cells all expressed immunoglobulin on their surface, but failed to secrete it. Over 200 different human-mouse somatic cell hybrids were obtained; 57% of them secreted human immunoglobulin in large amounts. Human immunoglobulin secretion can be a stable property of these hybrid cells over months of continuous culture. In each case the human immunoglobulin secreted was restricted to the light chain type expressed by the parental B leukemia cell. In addition, these hybrid cells secreted the original mouse myeloma protein and a variety of mixed human-mouse immunoglobulin molecules.
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism*; D002454:Cell Differentiation; D002460:Cell Line; D006801:Humans; D006822:Hybrid Cells / Q000378:metabolism*; D007147:Immunoglobulin Light Chains / Q000378:metabolism; D007136:Immunoglobulins / Q000378:metabolism*; D007938:Leukemia / Q000378:metabolism*; D007945:Leukemia, Lymphoid / Q000378:metabolism*; D051379:Mice; D009194:Myeloma Proteins / Q000378:metabolism
D007147:Immunoglobulin Light Chains; D007136:Immunoglobulins; D009194:Myeloma Proteins