(1-14C) acetate assimilation by obligate methylotrophs, Pseudomonas methanica and Methylosinus trichosporium.
The oxidation of one carbon compounds (methane, methanol, formaldehyde, formate) and primary alcohols (ethanol, propanol, butanol) supported the assimilation of [1-14C]acetate by cell suspensions of type I obligate methylotroph, Pseudomonas methanica, Texas strain, and type II obligate methylotroph, Methylosinus trichosporium, strain PG. The amount of oxygen consumed and substrate oxidized correlated with the amount of [1-14C]acetate assimilated during oxidation of C-1 compounds and primary alcohols. Oxidation of methanol, formaldehyde, and primary alcohols in extracts of Pseudomonas methanica, Texas strain, and Methylosinus trichosporium, strain PG, was catalyzed by a phenazine methosulfate linked, ammonium ion dependent methanol dehydrogenase. The oxidation of aldehydes was catalyzed by a phenazine methosulfate linked, ammonium ion independent aldehyde dehydrogenase. Formate was oxidized by a NAD+ linked formate dehydrogenase.
D000085:Acetates / Q000378:metabolism*; D000438:Alcohols / Q000378:metabolism; D000447:Aldehydes / Q000378:metabolism; D005557:Formaldehyde / Q000378:metabolism; D005561:Formates / Q000378:metabolism; D000432:Methanol / Q000378:metabolism; D008772:Methylococcaceae / Q000378:metabolism*; D011549:Pseudomonas / Q000378:metabolism*
D000085:Acetates; D000438:Alcohols; D000447:Aldehydes; D005561:Formates; D005557:Formaldehyde; D000432:Methanol