Genetic control of immunologic unresponsiveness to adjuvant-free solutions of beta-D-galactosidase. I. Inheritance of the Ir-Z1 and ir-Z2 loci in mice.
Two genetic loci, Ir-Z1 and ir-Z2, controlling the immune response to adjuvant-free bacterial beta-D-galactosidase (Z) are present in inbred mouse strains SJL/J and CE/J, respectively. Each locus segregates as a single, autosomal gene: Ir-Z1 as dominant and ir-Z2 as recessive. The response is characterized by production of activating and precipitating IgG. Maximal levels of circulating IgG occur between 16 and 20 days after immunization with a single, 50-microgram dose of enzyme. Failure of proteins other than Z to elicit an immune response indicates that the Ir-Z control is specific for determinant(s) of this enzyme. The immunogenicity of beta-D-galactosidase preparations cannot be attributed to either the catalytic activity of the enzyme or adjuvant contamination. Non-responder mice acquire immunologic memory without detectable increase in circulating specific IgG under the same conditions that elicit antibody production in responder strains.
D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000375:Aging; D000818:Animals; D000917:Antibody Formation; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D004307:Dose-Response Relationship, Radiation; D005260:Female; D005696:Galactosidases / Q000235:genetics*; D005799:Genes, Dominant; D007156:Immunologic Memory; D007700:Kinetics; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009154:Mutation; D001616:beta-Galactosidase / Q000235:genetics*
D000276:Adjuvants, Immunologic; D005696:Galactosidases; D001616:beta-Galactosidase