Positive control of ilvC expression in Escherichia coli K-12; identification and mapping of regulatory gene ilvY.
The construction of a plasmid carrying the ilvC::lacZ fusion is described. This plasmid provides a convenient source of template deoxyribonucleic acid for use in an in vitro protein-synthesizing system. We screened strains deleted in regions of the ilv cluster for their ability to support ilvC-dependent beta-galactosidase synthesis. The fact that two deletions prevented beta-galactosidase production indicated that ilv-C expression is under positive control. By use of plasmids carrying the positive-control factor structural gene ilvY, we were able to restore protein-synthesizing ability to these strains. These plasmids also enabled us to map ilvY between ilvA and ilvC.
D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D005809:Genes, Regulator; D005838:Genotype; D010957:Plasmids; D014176:Protein Biosynthesis; D014161:Transduction, Genetic; D001616:beta-Galactosidase / Q000096:biosynthesis
D004274:DNA, Recombinant; D004262:DNA Restriction Enzymes; D001616:beta-Galactosidase