Inactivation of the plasminogen activator from HeLa cells by peptides of arginine chloromethyl ketone.
The binding specificities of human urinary urokinase (EC 3.4.99.26) and HeLa cell plasminogen activator were studied using peptidyl chloromethyl ketone inhibitors. A 125I-labeled fibrin assay has been developed to yield kinetic information. Reagents of the sequence X-Gly-ArgCH2Cl were the most effective. The susceptibility of the HeLa cell plasminogen activator differed from that of urokinase in several respects indicating the utility of this type of inhibitor in distinguishing between proteases of this specificity.
D000590:Amino Acid Chloromethyl Ketones / Q000494:pharmacology*; D001120:Arginine / Q000031:analogs & derivatives; D001665:Binding Sites; D006367:HeLa Cells / Q000201:enzymology*; D006801:Humans; D007700:Kinetics; D009994:Osmolar Concentration; D010447:Peptide Hydrolases / Q000494:pharmacology; D010960:Plasminogen Activators / Q000037:antagonists & inhibitors*; D015849:Plasminogen Inactivators; D013379:Substrate Specificity; D014568:Urokinase-Type Plasminogen Activator / Q000037:antagonists & inhibitors
D000590:Amino Acid Chloromethyl Ketones; D015849:Plasminogen Inactivators; C020705:arginine chloromethyl ketone; D001120:Arginine; D010447:Peptide Hydrolases; D010960:Plasminogen Activators; D014568:Urokinase-Type Plasminogen Activator