title: Macrophage-T cell interactions involving Listeria monocytogenes--role of the H-2 gene complex.
abstract: This study reports on the in vitro interactions between T cells from Listeria-immunized mice, macrophages from normal mice, and heat-killed Listeria organisms. This interaction was assayed either by determing the amount of thymocyte mitogen in culture fluids after 24 hr, or by estimating the degree of T cell proliferation after 96 hr. Each assay depended on critical concentrations of macrophages, T cells, and heat-killed Listeria, points that were evaluated in a number of experiments. Both assays required specific Listeria-immune T cells. For an effective interaction, the T cells and the macrophages had to share the I-A region of the H-2 gene complex. Macrophages bearing Ia, which represented a minor population of macrophages, were essential for the proliferative response to macrophage-associated Listeria. Also, Ia-bearing macrophages were an important component in the interactions leading to increased secretion of mitogen. The immunogenic moiety associated with Listeria was short-lived, disappearing 24 hr after uptake of Listeria by macrophages. The interactions were not blocked by anti-Listeria antibodies but were partially sensitive to trypsinization.
mesh_terms: D000818:Animals; D000941:Antigens; D002417:Cattle; D002450:Cell Communication; D002478:Cells, Cultured; D003470:Culture Media; D005333:Fetus; D006183:H-2 Antigens / Q000235:genetics*; D008089:Listeria monocytogenes / Q000276:immunology*; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008805:Mice, Inbred A; D008810:Mice, Inbred C57BL; D008934:Mitogens / Q000378:metabolism; D011817:Rabbits; D013601:T-Lymphocytes / Q000276:immunology*; D013997:Time Factors
chemical_list: D000941:Antigens; D003470:Culture Media; D006183:H-2 Antigens; D008934:Mitogens