Purification and characterization of a kininase from human urine.
A kininase has been purified from male human urine which splits the C-terminal arginyl residue from bradykinin, but does not split hippuryl-L-arginine or converts Angiotensin I into Angiotensin II. It contains cadmium ion in its active center, and has a molecular weight of 210,000 by ultracentrifugation.
D002268:Carboxypeptidases / Q000652:urine*; D004492:Edetic Acid / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D001121:Lysine Carboxypeptidase / Q000302:isolation & purification / Q000652:urine*; D008297:Male; D008970:Molecular Weight; D013379:Substrate Specificity
D004492:Edetic Acid; D002268:Carboxypeptidases; D001121:Lysine Carboxypeptidase