Formation of spirodilactone of 4-(2'-carboxyphenyl)-4,4-dihydroxybutyrate from 2-succinylbenzoate in cell-free extracts of Micrococcus luteus.
The enzyme mediated ATP- and, to a lesser extent, CoASH-dependent synthesis of the spirodilactone of 4-(2'-carboxyphenyl)-4,4-dihydroxybutyrate from 2-succinylbenzoate has been demonstrated in membrane-free extracts of Micrococcus luteus and Escherichia coli. The suggestion is made that the spirodilactone is the product of an aberrant reaction involving a compound that is normally an intermediate in the conversion of 2-succinylbenzoate to 1,4-dihydroxy-2-naphthoate.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D002474:Cell-Free System; D003065:Coenzyme A / Q000494:pharmacology; D004926:Escherichia coli / Q000378:metabolism; D006885:Hydroxybutyrates / Q000096:biosynthesis*; D007783:Lactones / Q000096:biosynthesis; D008837:Micrococcus / Q000201:enzymology / Q000378:metabolism*; D008954:Models, Biological; D010654:Phenylbutyrates / Q000378:metabolism*; D011551:Pseudomonas fluorescens / Q000378:metabolism; D013141:Spiro Compounds / Q000096:biosynthesis*
D006885:Hydroxybutyrates; D007783:Lactones; D010654:Phenylbutyrates; D013141:Spiro Compounds; C013691:o-succinylbenzoic acid; D000255:Adenosine Triphosphate; D003065:Coenzyme A