A modified assay for studies of cultured granulocyte precursors: cryopreservation of stimulating mononuclear cells.
Mononuclear cells (MNC), isolated from peripheral blood of healthy donors, were cryoprotected by dimethyl sulfoxide and stored in liquid nitrogen. Colony-stimulating factor (CSF), produced by cryopreserved MNC, was compared with that of nonfrozen controls in a double-layer agar system with human bone marrow as target cells. Our results indicate that cryopreserved MNC retain their ability to stimulate myelopoiesis-committed stem cells after freezing. In addition, evidence was obtained that CSF of feeder layers changes, depending on the duration of preincubation and cell concentration. In a system where either stimulating or target cells are cryopreserved the dynamics of interactions between normal or abnormal cell lines can thus be studied.
D001793:Blood Preservation; D001854:Bone Marrow Cells; D002455:Cell Division; D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000096:biosynthesis; D003451:Cryoprotective Agents; D004121:Dimethyl Sulfoxide; D006098:Granulocytes / Q000166:cytology / Q000502:physiology*; D006801:Humans; D009584:Nitrogen
D003115:Colony-Stimulating Factors; D003451:Cryoprotective Agents; D009584:Nitrogen; D004121:Dimethyl Sulfoxide