A novel method in enzyme immunoassay: maleimide derivative of hapten for enzyme coupling.
Meta-maleimidobenzoyl derivative of L-thyroxine methyl ester (MBTM) was synthesized and coupled to beta-galactosidase at molar ratio of over 5 to 1. More than 97% of the enzyme was found to be labeled with MBTM. A thyroxine enzyme immunoassay was carried out with sensitivity in 0-10 microgram/100 ml range.
D000818:Animals; D006041:Goats; D006241:Haptens; D007124:Immunoenzyme Techniques; D008299:Maleic Anhydrides; D011817:Rabbits; D013974:Thyroxine / Q000032:analysis; D001616:beta-Galactosidase
D006241:Haptens; D008299:Maleic Anhydrides; D001616:beta-Galactosidase; D013974:Thyroxine