In vitro sensitization of thymocytes. Role of H-21 I region determinants and cell-free mixed leukocyte culture supernates in generation of cytotoxic responses.
Stimulation of thymocytes in vitro by spleen cells differing for the entire H-2 complex leads to a significant proliferative response without a significant cell-mediated lympholysis (CML) response. Addition of soluble cell-free supernates (SF), (taken from a 7-day mixed leukocyte culture) enables these cultures to develop CML response. For optimal CML response, the SF has to be added within 48 h of onset of cultures. Although with spleen cells as responding cells, SF could quantitatively replace I-region different stimulating cells for generation of CML responses, with thymocytes as responding cells, stimulation with I-region cells appeared obligatory for the generation of CML responses. The implications of these findings are discussed.
D000818:Animals; D003602:Cytotoxicity, Immunologic; D005260:Female; D005802:Genes, MHC Class II; D006183:H-2 Antigens / Q000235:genetics*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D008285:Major Histocompatibility Complex; D008297:Male; D051379:Mice; D013154:Spleen / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology
D006183:H-2 Antigens