Simple method to detect virus-specific IgM antibodies in patients' serum samples after immunosorption of immunoglobulins G and A.
By absorption with protein A and specific immunosorption using insolubilized adsorbentia (CPG), a method was developed for rapid elimination of IgG and IgA from patients' serum samples. This test technique was examined in the diagnosis of rubella infections, i.e., rubella-specific IgM antibodies. The procedure has proven to be as reliable as determinations carried out with IgM fractions separated on a sucroe density gradient.
D000914:Antibodies, Viral / Q000032:analysis*; D003937:Diagnosis, Differential; D006386:Hemagglutination Tests; D006801:Humans; D005779:Immunodiffusion; D007070:Immunoglobulin A / Q000302:isolation & purification; D007075:Immunoglobulin M / Q000032:analysis* / Q000302:isolation & purification; D007163:Immunosorbent Techniques; D012409:Rubella / Q000175:diagnosis*; D012412:Rubella virus / Q000276:immunology*
D000914:Antibodies, Viral; D007070:Immunoglobulin A; D007075:Immunoglobulin M