[Microbial metabolic activity and transmembrane transport phenomena by potentiometric analysis of lipoic acid oxidation-reduction, in a minimal culture medium].
A method of measuring and studying metabolic bacterial activity is proposed, by following the kinetic evolution of the ratio of the oxidized and reduced forms of an electron transporter as a consequence of decreasing oxidizing power--due to oxygen consumption in the culture,--and increasing. Reduction power of bacterial activity. Namely, with minimum composition using salts and glucose the oxido-reduction of lipoic acid is well indicated by a gold electrode without any major bio-or electrochemical interference. A kinetic diffusion reaction theory takes into account the passive or active transmembrane transport of lipoic acid in good agreement with the experimentally observed shapes of the electrical signal. The various types of antibiotic activities are well reflected by the modifications of the signal.
D001419:Bacteria / Q000378:metabolism*; D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism; D003470:Culture Media; D004579:Electron Transport; D007700:Kinetics; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D011199:Potentiometry; D013816:Thermodynamics; D008063:Thioctic Acid / Q000378:metabolism*
D003470:Culture Media; D008063:Thioctic Acid
Mesure de l'activité métabolique microbienne et des phénoménes de transports transmembranaires par analyse potentiométrique de l'oxydoréduction de l'acide lipoïque, dans un milieu de culture minimal.