[Substrate specificity of lipase from Pseudomonas fluorescens].
Substrate specificity of lipase isolated from the culture liquid filtrate of Pseudomonas fluorescens BKM-B-1151 was investigated with respect to vegetable oils and animal fats (olive, sunflower, cotton, mustard and soybean oils; beef and hog fats and their glycerides and fatty acid esters). The preparation showed a high specificity to the quantitative composition of the reaction mixture (substrate: enzyme ratio), chemical structure of the substrate, and the emulgator type (gelatine, gum arabic and Triton X-100). The lipase preparation hydrolyzed oils and water-insoluble fatty acid esters. The latter indicated an involvement of lipase.
D007700:Kinetics; D008049:Lipase / Q000378:metabolism*; D011551:Pseudomonas fluorescens / Q000201:enzymology*; D013379:Substrate Specificity
D008049:Lipase
Substratnaia spetsifichnost' lipazy Pseudominas fluorescens.