Isolation and characterization of cDNA complementary to transient maternal poly(A)+ RNA from the Drosophila oocyte.
cDNA complementary to total oocyte poly(A)+ RNA from Drosophila melanogaster was enriched for sequences complementary to transient maternal sequences; that is, those sequences which disappear from the oocyte during subsequent. A seven- to ten-fold enrichment factor was obtained, from 5.3% to about 50% of the total cDNA. Kinetic analysis of this enriched fraction indicates that the transient maternal sequences include 44 +/- 14 different sequences.
D000818:Animals; D001483:Base Sequence; D004247:DNA / Q000302:isolation & purification*; D004331:Drosophila melanogaster / Q000196:embryology; D005260:Female; D009693:Nucleic Acid Hybridization; D009865:Oocytes / Q000032:analysis*; D010063:Ovum / Q000032:analysis*; D011061:Poly A / Q000032:analysis* / Q000302:isolation & purification; D012313:RNA / Q000032:analysis* / Q000302:isolation & purification
D011061:Poly A; D012313:RNA; D004247:DNA