Simultaneous competitive enzyme immunoassay for human chorionic gonadotropin.
A simultaneous competitive enzyme immunoassay (SICEIA) for hCG was developed using beta-D-galactosidase (beta-Gal) as a labelled enzyme and anti-hCG antibody coated sheep red blood cells (SRBC) as a solid phase. In this report, a new coupling agent, MCAE, was used to couple beta-Gal with hCG. The sensitivity was improved to the degree of 2.5 mIU/ml, equal to that of RIA. The present procedure was safer and rapider than RIA. The value of hCG in urine by our procedure had good correlation with that by RIA.
D001667:Binding, Competitive; D006063:Chorionic Gonadotropin / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D001616:beta-Galactosidase
D006063:Chorionic Gonadotropin; D001616:beta-Galactosidase