Electrophoretic cell separation by means of microspheres.
The electrophoretic mobility of fixed human erythrocytes immunologically labeled with poly(vinylpyridine) or poly(glutaraldehyde) microspheres was reduced by approximately 40%. This observation was utilized in preparative scale electrophoretic separations of fixed human and turkey erythrocytes, the mobilities of which under normal physiological conditions do not differ sufficiently to allow their separation by continuous flow electrophoresis. We suggest that resolution in the electrophoretic separation of cell subpopulations, currently limited by finite and often overlapping mobility distributions, may be significantly enhanced by immunospecific labeling of target populations using microspheres.
D000818:Animals; D002469:Cell Separation / Q000379:methods*; D004586:Electrophoresis; D004912:Erythrocytes / Q000032:analysis* / Q000276:immunology / Q000648:ultrastructure; D005976:Glutaral / Q000031:analogs & derivatives / Q000494:pharmacology; D006801:Humans; D008863:Microspheres; D011108:Polymers / Q000494:pharmacology; D011145:Polyvinyls / Q000494:pharmacology; D011725:Pyridines / Q000494:pharmacology; D014422:Turkeys
D011108:Polymers; D011145:Polyvinyls; D011725:Pyridines; C022841:poly(glutaraldehyde); D005976:Glutaral