Preparation of an alloantiserum to murine C3 and demonstration of multiple alleles.
An antiserum was produced in C3H/He mice that can recognize the antigenic variations of murine C3. The alloantiserum is directed to the gene product of one of the alleles (C3-1a allele) of the C3-1 locus that controls genetic variations of murine C3 and is linked to H-2. The antiserum formed a single precipitin line in the Ouchterlony test with sera obtained from mice that carry the C3-1a/a or C3-1a/b genotype, but it did not react with sera from mice that do not have C3 coded for by the C3-1a allele. By the use of the alloantiserum, we detected a new C3 allotype (C3-1 CC) in SWR/J mice. The C3-1 CC is distinct from the other two allotypes on the basis of antigenicity and isoelectric point (pI). Therefore, at least three alleles have been identified for the C3-1 locus.
D000483:Alleles; D000818:Animals; D003176:Complement C3 / Q000235:genetics; D003433:Crosses, Genetic; D007106:Immune Sera; D005779:Immunodiffusion; D007518:Isoantibodies; D007525:Isoelectric Focusing; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D010641:Phenotype; D011110:Polymorphism, Genetic
D003176:Complement C3; D007106:Immune Sera; D007518:Isoantibodies