[Expression of a bacterial gene, cloned in the yeast, Saccharomyces cerevisiae].
Vectors allowing cloning of foreign D.N.A. in the yeast Saccharomyces cerevisiae have been recently described. We have introduced in this yeast the lac Z gene of the bacteria Escherichia coli. An active beta-galactosidase, which is absent in the recipient strain, has been detected in transformed yeast. We thus conclude that the bacterial lac Z gene is expressed in yeast. We further showed that the enzyme found in the transformed yeast is identical to the bacterial enzyme with respect to size and immunological criteria.
D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics*; D005696:Galactosidases / Q000235:genetics*; D007763:Lac Operon; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics*
D005696:Galactosidases; D001616:beta-Galactosidase
Sur l'expression d'un gène bactérien cloné dans la levure Saccharomyces cerevisiae.