Specificity of cytochemical procedures for localising peroxidase activity in the sarcoplasmic reticulum.
Cytochemical evidence is reported for substantiating the view that when lightly-fixed skeletal muscle is incubated in a diaminobenzidine-H2O2 medium at pH 5, the resulting enhanced electron opacity of the sarcoplasmic reticulum is more likely to be due to a peroxidatic activity therein rather than to a non-enzymic binding reaction. The reticulum staining is absent in incubated sections of overfixed or boiled tissue; or if hydrogen peroxide is omitted from the incubation medium; or if aminotriazole is included in the medium.
D000818:Animals; D006224:Cricetinae; D006651:Histocytochemistry; D009132:Muscles / Q000201:enzymology / Q000648:ultrastructure; D010544:Peroxidases / Q000032:analysis*; D012519:Sarcoplasmic Reticulum / Q000201:enzymology* / Q000648:ultrastructure; D013194:Staining and Labeling
D010544:Peroxidases