title: The primary structure of Lactobacillus casei thymidylate synthetase. II. The complete amino acid sequence of the active site peptide, CNBr 4.
abstract: The 102 amino acid residues of CNBr 4, the largest of 5 cyanogen bromide peptides from the Lactobacillus casei thymidylate synthetase were completely sequenced by means of limited tryptic, tryptic, chymotryptic, and staphylococcal protease peptides. CNBr 4 contains both of the cysteines in an enzyme subunit, with the 5-fluorodeoxyuridylate-reactive cysteine at residue 198 and the other at residue 244.
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D001665:Binding Sites; D003488:Cyanogen Bromide; D007780:Lacticaseibacillus casei / Q000201:enzymology*; D008780:Methyltransferases; D010446:Peptide Fragments / Q000032:analysis; D013940:Thymidylate Synthase
chemical_list: D000596:Amino Acids; D010446:Peptide Fragments; D008780:Methyltransferases; D013940:Thymidylate Synthase; D003488:Cyanogen Bromide