Use of EDTA blood samples for mannosidosis testing.
Preincubation of EDTA plasma with 4mM ZnCl2 restores acidic alpha mannosidase activity, previously inhibited as a result of chelation of Zn++ by EDTA. This allows estimation of acidic alpha mannosidase activity in EDTA plasma samples, followed by granulocyte tests on those samples with low activities.
D000818:Animals; D001793:Blood Preservation / Q000662:veterinary; D002239:Carbohydrate Metabolism, Inborn Errors / Q000175:diagnosis / Q000662:veterinary*; D002417:Cattle / Q000097:blood*; D002418:Cattle Diseases / Q000175:diagnosis*; D004796:Clinical Enzyme Tests / Q000662:veterinary; D004492:Edetic Acid; D008361:Mannosidases / Q000097:blood*; D015032:Zinc / Q000494:pharmacology
D004492:Edetic Acid; D008361:Mannosidases; D015032:Zinc