Immunochemical comparison of histidine-rich protein in keratohyalin granules and cornified cells.
(1) Combination of techniques for extraction and purification of histidine rich protein established by several investigators were employed for comparison of histidine-rich protein in granular cells and cornified cells of newborn rats. (2) Histidine-rich protein extracted from the same cell fraction by two different techniques either in 1 M potassium phosphate buffer (Ugel) or in 4 M urea (Dale) showed identical elution profiles on CM 52 cellulose ion exchange chromatography and the same SDS polyacrylamide gel electrophoretic patterns. (3) Histidine-rich protein from granular cells contained polypeptides of larger molecular sizes than those in histidine-rich protein from cornfield cells, although amino acid composition of the two histidine-rich protein was non-distinguishable (histidine residue was more than 7%). (4) Antibodies raised in rabbits by injection of histidine rich protein from granular cells and that from cornfield cells immunologically cross-reacted. Furthermore, the antisera were found to be reactive over both keratohyalin granules and cornified cells, but not epidermal cells of the lower strata.
D000818:Animals; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D006639:Histidine / Q000032:analysis* / Q000276:immunology; D006818:Hyalin / Q000032:analysis*; D007120:Immunochemistry; D007633:Keratins / Q000032:analysis*; D011506:Proteins / Q000032:analysis* / Q000276:immunology; D051381:Rats; D012867:Skin / Q000032:analysis*; D012995:Solubility
D011506:Proteins; D006639:Histidine; D007633:Keratins