Immunohistological staining of antigens on semithin sections of specimens embedded in plastic (GMA-Quetol 523).
Glycol methacrylate-Quetol 523, introduced by Kushida (1977) for combined light and electronmicroscopy studies at low magnification, also permits application of immunofluorescence methods to semithin sections. To recover the antigenicity of proteins fixed with formaldehyde, abrupt dehydration before embedding and subsequent treatment of the semithin sections with protease were essential. Post-staining with suitable histological stains allows exact correlation of antigen localization with tissue structure.
D000180:Acrylic Resins; D000818:Animals; D000941:Antigens; D000946:Antigens, Fungal; D001485:Basement Membrane / Q000276:immunology; D002176:Candida albicans / Q000276:immunology; D005293:Ferritins / Q000276:immunology; D005455:Fluorescent Antibody Technique / Q000379:methods*; D005557:Formaldehyde / Q000494:pharmacology; D005629:Frozen Sections; D005976:Glutaral / Q000494:pharmacology; D007136:Immunoglobulins / Q000276:immunology; D007678:Kidney Glomerulus / Q000276:immunology; D008867:Microtomy; D011102:Polyhydroxyethyl Methacrylate; D011817:Rabbits; D051381:Rats
D000180:Acrylic Resins; D000941:Antigens; D000946:Antigens, Fungal; D007136:Immunoglobulins; D005557:Formaldehyde; D011102:Polyhydroxyethyl Methacrylate; D005293:Ferritins; D005976:Glutaral