[Preservation of hematopoietic stem cells by slow freezing and elimination of the heat of fusion].
Previous experiments, in dogs with fresh bone marrow or bone marrow frozen with a modified freezing system have demonstrated a 100% recovery of frozen stem cells, stored for periods up to 5 months. Five patients, three with drug resistant acute leukemia  and two with metastic carcinomas, have been treated with a high dose combination chemotherapy regimen (TACC) followed by reinfusion of marrow cryopreserved with the same modified freezing system. Following the reinfusion of marrow, autologous engraftment was demonstrated on bone marrow aspiration between days 5 and 10.
D000328:Adult; D000818:Animals; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D004285:Dogs; D004359:Drug Therapy, Combination; D005260:Female; D005615:Freezing; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D007938:Leukemia / Q000628:therapy; D008297:Male; D008875:Middle Aged; D009362:Neoplasm Metastasis; D013997:Time Factors; D014021:Tissue Preservation
Conservation de cellules souches hématopoïétiques par congélation lente et élimination de la chaleur de fusion.