Structural and antigenic studies of an idiotype-bearing murine antibody to the arsonate hapten.
Mice of strain A/J responded to repeated intraperitoneal injection of Limulus hemocyanin derivatized with arsanilic acid by producing large quantities (approximately 5 mg/mL of ascites fluid) of IgG antibodies specific for this hapten. The antibodies possessed a characteristic idiotypic determinant and exhibited restricted heterogeneity as demonstrated by isoelectric focusing and primary N-terminal amino acid sequence analysis of isolated light and heavy polypeptide chains. Both light- and heavy-chain sequences were comparable to those of myeloma proteins in lack of heterogeneity. The N terminus of the light chain exhibited V KI sequence and only one position in the first 30 residues showed more than one amino acid. No variability was observed in the first 10 N-terminal residues of the heavy chain. Rabbit antiserum to the idiotype blocked binding of hapten by the purified antibody. The presence of both light- and heavy-chain antigenic determinants was required for optimal formation of the idiotypic determinant.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D000906:Antibodies; D000951:Antigens, Neoplasm; D001147:Arsanilic Acid / Q000276:immunology*; D001152:Arsenicals / Q000276:immunology*; D006241:Haptens; D007143:Immunoglobulin Heavy Chains; D007130:Immunoglobulin Idiotypes; D007147:Immunoglobulin Light Chains; D007145:Immunoglobulin kappa-Chains; D051379:Mice; D008815:Mice, Inbred Strains; D011863:Radioimmunoassay
D000596:Amino Acids; D000906:Antibodies; D000951:Antigens, Neoplasm; D001152:Arsenicals; D006241:Haptens; D007143:Immunoglobulin Heavy Chains; D007130:Immunoglobulin Idiotypes; D007147:Immunoglobulin Light Chains; D007145:Immunoglobulin kappa-Chains; D001147:Arsanilic Acid