Cholesterol in high-density lipoproteins: a comparison between dextran sulfate-magnesium chloride precipitation and preparative ultracentrifugation.
The cholesterol in high density lipoproteins (HDL) has been determined in 140 serum samples after dextran sulfate-magnesium chloride precipitation of the apolipoprotein B-containing lipoproteins. The values correlated well with simultaneously measured values from preparative ultracentrifugation (slope 0.98, r = 0.93). Furthermore, there was a significant correlation in 60 specimen between the dextran sulfate-MgCl2 and heparin-MnCl2-precipitation method.
D011232:Chemical Precipitation; D002712:Chlorides; D002784:Cholesterol / Q000097:blood*; D016264:Dextran Sulfate; D003911:Dextrans; D006493:Heparin; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood; D008274:Magnesium; D015636:Magnesium Chloride; D008345:Manganese; D017895:Manganese Compounds; D008722:Methods; D008875:Middle Aged; D014461:Ultracentrifugation / Q000379:methods
D002712:Chlorides; D003911:Dextrans; D008075:Lipoproteins, HDL; D017895:Manganese Compounds; D015636:Magnesium Chloride; D008345:Manganese; D006493:Heparin; D016264:Dextran Sulfate; D002784:Cholesterol; D008274:Magnesium; C025340:manganese chloride