[Differential interaction of 2,4,6-trinitrobenzenesulfonic (TNBS) and 2,4-dinitrobenzenesulfonic (DNBS) acids on mouse lymphocytes].
Modifications of lymphoid cells with dinitro-2,4 phenylsulfonic acid (DNBS) or trinitro-2,4,6 phenylsulfonic acid (TNBS) have been studied. TNBS action always produces an electrophoretic mobility increase in relation with the amount of amino-groups, according to the cell type. DNBS action produces an electrophoretic mobility increase for B cells of spleen and a decrease for T cells of spleen and thymic cells. The hydrophobic fluorescent probe 1-anilino-8-naphthalene sulphonate used to study conformational changes of cells revealed a slight fluorescence decrease for TNP-modified cells and an important fluorescence increase for DNP-modified cells.
D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects; D001557:Benzenesulfonates / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects; D004136:Dinitrobenzenes / Q000494:pharmacology; D004586:Electrophoresis; D005453:Fluorescence; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000187:drug effects*; D051379:Mice; D008809:Mice, Inbred C3H; D009578:Nitrobenzenes / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000187:drug effects; D014302:Trinitrobenzenesulfonic Acid / Q000494:pharmacology*
D001557:Benzenesulfonates; D004136:Dinitrobenzenes; D009578:Nitrobenzenes; D014302:Trinitrobenzenesulfonic Acid
Interaction différentielle des acides trinitro-2,4,6 benzène sulfonique (TNBS) et dinitro-2,4 benzène sulfonique (DNBS) sur des lymphocytes de Souris.