Stereotyped behavior and hyperthermia in dogs: correlation with the levels of amphetamine and p-hydroxyamphetamine in plasma and CSF.
A gas-chromatographic method for simultaneously measuring p-hydroxyamphetamine (pOA) against amphetamine (A) in plasma and CSF is presented. The time course of body temperature (Tb), stereotyped behavior (St), and A and pOA levels in plasma and CSF were studied after administration of 0.6 and 1.5 mg/kg p.o. of A to dogs. Stereotyped behavior reached maximal value 2.5 h after A, as did levels of A in CSF. The A levels in CSF decreased steadily in the following hours and simultaneously with the levels of A in plasma. St remained elevated and began to decrease after 6.5 h. The relationship between St and amounts of A was not linear but exponential. This suggest that both A and its metabolite contributed to this effect. In fact, a linear relationship was found between St and the amounts of pOA in CSF. Body temperature had a time course similar to A plasma levels, reaching peak value after 1.5 h and declining thereafter simultaneously with A. A linear relationship was found between Tb and the amounts of A in plasma. Thus Tb seems to be a peripheral A effect related to the presence of the drug in plasma.
D000661:Amphetamine / Q000097:blood / Q000134:cerebrospinal fluid / Q000378:metabolism / Q000494:pharmacology*; D000662:Amphetamines / Q000378:metabolism*; D000818:Animals; D001519:Behavior / Q000187:drug effects*; D001831:Body Temperature / Q000187:drug effects*; D004285:Dogs; D006801:Humans; D007700:Kinetics; D008297:Male; D013239:Stereotyped Behavior / Q000187:drug effects*; D010136:p-Hydroxyamphetamine / Q000097:blood / Q000134:cerebrospinal fluid / Q000378:metabolism*
D000662:Amphetamines; D000661:Amphetamine; D010136:p-Hydroxyamphetamine