The effect of disodium cromoglycate on in vitro mast cell degranulation in human jejunal mucosa.
The in vitro effect of disodium cromoglycate (DSCG) on IgE antigen-induced mast cell degranulation is described. Serum from an egg white allergic patient was used to sensitize jejunal mucosa from nine individuals. Egg white was used to challenge the IgE sensitized mast cells. DSCG in concentrations 3 x 10(-7) M to 3 x 10(-4) M was added to the mucosal specimens before antigen challenge. Mast cell degranulation in the sensitized specimens challenged with egg white was 38%. Mast cell degranulation in sensitized specimens treated with DSCG before and during antigen challenge was reduced to 2% at a concentration of 3 x 10(-5) M of DSCG (P=0.006) and to 28% at a concentration of 3 x 10(-6) M (P=0.027). No significant reduction of mast cell degranulation was seen at concentrations of 3 x 10(-7) M and 3 x 10(-4) M. The results support a role for DSCG in the treatment of gastrointestinal allergy.
D000941:Antigens; D004205:Cromolyn Sodium / Q000276:immunology / Q000494:pharmacology*; D006801:Humans; D006967:Hypersensitivity; D007114:Immunization; D007073:Immunoglobulin E; D007413:Intestinal Mucosa / Q000187:drug effects / Q000276:immunology; D007583:Jejunum / Q000276:immunology; D008407:Mast Cells / Q000187:drug effects* / Q000276:immunology
D000941:Antigens; D007073:Immunoglobulin E; D004205:Cromolyn Sodium