[Determinaton of intracellular immunoglobulins by means of immunofluorescence. 1. The standardization of monospecific luminescent antisera against human G-, A- and M-class immunoglobulins in bone marrow preparations from patients with paraproteinemic hemoblastosis].
To assess the specificity and the activity of fluorescent antisera against human G-, A-, M-classes the authors used in the capacity of a substrate, containing specific antigen preparations, bone marrow cells of patients with paraproteinemic hemoblastosis. The method of making the preparations used permitted sufficiently objective assessment of the activity and specificity of the mentioned monospecific fluorescent conjugates. As revealed, the bone marrow preparations could be stored at -70 degrees C for over one year.
D000888:Antibodies, Anti-Idiotypic / Q000032:analysis; D000918:Antibody Specificity; D001853:Bone Marrow / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D007106:Immune Sera / Q000592:standards*; D007111:Immunity, Cellular; D007070:Immunoglobulin A / Q000032:analysis*; D007074:Immunoglobulin G / Q000032:analysis*; D007075:Immunoglobulin M / Q000032:analysis*; D009101:Multiple Myeloma / Q000276:immunology; D010265:Paraproteinemias / Q000276:immunology*; D008258:Waldenstrom Macroglobulinemia / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D007106:Immune Sera; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M
Opredelenie vnutrikletochnykh immunoglobulinov metodom immunofliuorestsentsii. Soobshchenie 1. Standartizatsiia monospetsificheskikh liuminestsiruiushchikh antisyvorotok protiv immunoglobulinov cheloveka G-, A-, i M-klassov na preparatakh kostnogo mozga bol'nykh paraproteinemicheskimi gemoblastozami.