Hormonal control of gene expression: differential activation of rat bone marrow RNA polymerases by erythropoietin and testosterone.
Hormones play a role in the regulation of gene expression by inducing changes in enzyme patterns in target cells mediated by the synthesis of specific RNA molecules. Erythropoiesis has been used as a system for studying the molecular mechanism of regulation of gene action by means of two hormones: erythropoietin and testosterone. Experiments designed to correlate the biochemical action of both hormones on rat marrow cells are herein reported. Both factors seems to act at different biochemical and citological levels. Erythropoietin triggers the erythropoietic process acting on the erythropoietin sensitive cells (ESC), in which the hormone induces the synthesis of a high molecular weight RNA, which is the precursor of a functional 9 S messenger RNA. Testosterone seems to act on polychromatophilic erythroblasts, in which the synthesis of ribosomal RNA or its precursor is stimulated. The steroid enhances the nuclear ribonuclease activity, which could represent a control mechanism for the processing (maturation) of high molecular weight RNAs. The incorporation of 3H-GTP and 3H-UTP into RNA by isolated rat bone marrow nuclei is stimulated by erythropoietin and testosterone. Using alpha-amanitine and different ionic strength conditions it was found that erythropoietin enhances preferentially RNA polymerase II activity while testosterone increases RNA polymerase I activity. It is postulated that erythropoietin and testosterone act synergically to create the biochemical machinery for hemoglobin synthesis, the macromolecule that characterizes the erythropoietic process.
D000546:Amanitins / Q000494:pharmacology; D000818:Animals; D001853:Bone Marrow / Q000201:enzymology*; D002454:Cell Differentiation / Q000187:drug effects; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004357:Drug Synergism; D004921:Erythropoietin / Q000502:physiology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D006160:Guanosine Triphosphate / Q000378:metabolism; D008954:Models, Biological; D012313:RNA / Q000378:metabolism; D012318:RNA Polymerase I / Q000378:metabolism; D012319:RNA Polymerase II / Q000037:antagonists & inhibitors / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D012335:RNA, Ribosomal / Q000096:biosynthesis; D051381:Rats; D013739:Testosterone / Q000502:physiology*; D014158:Transcription, Genetic; D014544:Uridine Triphosphate / Q000378:metabolism
D000546:Amanitins; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D004921:Erythropoietin; D013739:Testosterone; D012313:RNA; D006160:Guanosine Triphosphate; D012319:RNA Polymerase II; D012321:DNA-Directed RNA Polymerases; D012318:RNA Polymerase I; D014544:Uridine Triphosphate