RNA synthesis in starved deciliated Tetrahymena pyriformis.
Tetrahymena pyriformis which has been starved for 20 h by incubation in buffer, and then deciliated, can regenerate its cilia in about 90 min while still in suspension in non-nutrient medium. The process of reciliation is accompanied by protein synthesis which begins a few minutes after deciliation and by synthesis of ribosomal and messenger RNAs during a period extending from about 1 h to about 3 h after deciliation. Although net synthesis of RNA remains at a very low level until 1 h after deciliation, a qualitative change in the translatable poly(A)-containing messenger RNA content of deciliated cells, and in particular, formation of beta-tubulin mRNA can be detected almost immediately after deciliation.
D000818:Animals; D002923:Cilia / Q000502:physiology*; D011132:Polyribosomes / Q000378:metabolism; D012313:RNA / Q000096:biosynthesis*; D013769:Tetrahymena pyriformis / Q000378:metabolism* / Q000502:physiology; D014158:Transcription, Genetic
D012313:RNA