Separation of algal mixtures and bacterial mixtures with flow-microfluorometer using chlorophyll and ethidium bromide fluorescence.
The applicability of flow-microfluorometer to separate microbial cells was demonstrated with algal and bacterial cells. Algal mixtures were sorted according to the natural chlorophyll fluorescence and the bacterial mixtures were sorted according to the fluorescence of ethidium bromide-stained nucleic acid.
D001412:Bacillus subtilis / Q000032:analysis / Q000302:isolation & purification; D001419:Bacteria / Q000302:isolation & purification*; D002734:Chlorophyll / Q000032:analysis; D004996:Ethidium; D056890:Eukaryota / Q000032:analysis / Q000302:isolation & purification*; D005453:Fluorescence; D005470:Fluorometry / Q000379:methods*; D009696:Nucleic Acids / Q000032:analysis; D012231:Rhizobium / Q000032:analysis / Q000302:isolation & purification
D009696:Nucleic Acids; D002734:Chlorophyll; D004996:Ethidium