Growth and sporulation of auxotrophs of Bacillus subtilis in a medium with limited nutrients.
Growth and sporulation were examined for 30 auxotrophs of Bacillus subtilis in a chemically defined medium with suboptimal amounts of nutrients. All strains except for some adenine-requiring mutants could not overtake sporulation stage II when amino acids, vitamins, or bases were limited, whereas they sporulated fairly well without limitation. Abnormal structures, a cell with thickened cell wall and a cell with several refractile bodies, were found in some strains after the vegetative growth stopped.
D000225:Adenine / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D001412:Bacillus subtilis / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D003470:Culture Media; D009154:Mutation; D013171:Spores, Bacterial; D014815:Vitamins / Q000378:metabolism
D000596:Amino Acids; D003470:Culture Media; D014815:Vitamins; D000225:Adenine