DNA repair synthesis in rat retinal ganglion cells treated with chemical carcinogens or ultraviolet light in vitro, with special reference to aging and repair level.
A system in which the retinal tissues of noninbred Wistar rats were used in combination with autoradiography was developed for measurement of DNA repair synthesis in ganglion cells of the central nervous system. Retinal tissues in short-term organ culture were treated with various carcinogens plus tritiated thymidine ([methyl-3H]dThd) or were irradiated with UV light and then treated with [methyl-3H]dThd. Preliminary study with retinal tissues from rats at various ages revealed no age-associated changes in the levels of unscheduled DNA synthesis in ganglion cells.
D015112:4-Nitroquinoline-1-oxide / Q000494:pharmacology; D000367:Age Factors; D000818:Animals; D002273:Carcinogens / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis*; D004260:DNA Repair / Q000187:drug effects* / Q000528:radiation effects; D005038:Ethylnitrosourea / Q000494:pharmacology; D005724:Ganglia / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D008297:Male; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D051381:Rats; D012160:Retina / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D014466:Ultraviolet Rays
D002273:Carcinogens; D015112:4-Nitroquinoline-1-oxide; D004247:DNA; D008741:Methyl Methanesulfonate; D005038:Ethylnitrosourea