Improved utilization of tissue blocks for electron microscopy. Effect of various concentrations of formaldehyde and glutaraldehyde.
Liver tissue from miniature pig fetuses was immersion-fixed in fixative mixtures with various concentrations of formaldehyde and glutaraldehyde. The preservation quality of hepatocytes was evaluated ultrastructurally in a peripheral zone (30--130 micron below the surface) and a central zone (500 micron below the surface). In the peripheral zone the best preservation was obtained with a fixative mixture containing 2% formaldehyde and 2% glutaraldehyde and in the central zone with a fixative mixture containing 8% formaldehyde and 8% glutaraldehyde. It is concluded that a better utilization of fairly large tissue blocks for ultrastructural investigation can be obtained by division of the block and subsequent fixation in fixatives containing various concentrations of formaldehyde and glutaraldehyde.
D000447:Aldehydes; D000818:Animals; D005404:Fixatives; D005557:Formaldehyde; D005976:Glutaral; D008099:Liver / Q000166:cytology / Q000196:embryology / Q000648:ultrastructure*; D008854:Microscopy, Electron / Q000379:methods*; D009926:Organ Preservation; D013552:Swine; D014021:Tissue Preservation
D000447:Aldehydes; D005404:Fixatives; D005557:Formaldehyde; D005976:Glutaral