title: Detection of protein A-like substances on haemolytic streptococci prior to use in mixed reverse passive antiglobulin haemagglutination (MRPAH).
abstract: Before mixed reverse passive antiglobulin haemagglutination tests (MRPAH) can be used to measure the class of bacterial antibodies, the bacteria have to be shown to be free of Protein A or Protein A-like substances on their surfaces. Two basic procedures have been examined: haemagglutination of red cells coated with immunoglobulin by the bacteria, and the MRPAH reaction itself to reveal absorption of purified gamma Fc by the bacterial suspension. The use of a purified gamma Fc component has proved successful in providing a sensitive test for the detection of Protein A-like substances on the surface of bacterial. In addition to both the Cowan and Wood strains of Staph, aureus, strains of haemolytic streptococci of groups A, C and G had Protein A-like substances on their surfaces. In contrast, strains of group B and group D, as well as Strep. milleri, had no detectable Protein A-like activity.
mesh_terms: D000042:Absorption; D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D004912:Erythrocytes / Q000276:immunology; D006386:Hemagglutination Tests; D006461:Hemolysis; D006801:Humans; D007074:Immunoglobulin G; D011817:Rabbits; D011961:Receptors, Fc / Q000276:immunology; D012204:Rh-Hr Blood-Group System; D012756:Sheep; D013205:Staphylococcal Protein A; D013291:Streptococcus / Q000276:immunology*; D013552:Swine; D014357:Trypsin / Q000494:pharmacology
chemical_list: D000888:Antibodies, Anti-Idiotypic; D007074:Immunoglobulin G; D011961:Receptors, Fc; D012204:Rh-Hr Blood-Group System; D013205:Staphylococcal Protein A; D014357:Trypsin