A comparison of the ability of beta-galactosidase and horseradish peroxidase enzyme-antibody conjugates to detect specific antibodies.
The ability of two different enzyme-antibody conjugates to detect specific antibodies has been compared. beta-Galactosidase was conjugated to antibodies raised against rabbit Fc fragments using m-maleimidobenzoyl-N-hydroxysuccinimide ester (MBS). Horseradish peroxidase (HRP) was conjugated to part of the same batch of antibodies using the periodate method. The beta-galactosidase and HRP labels enabled detection of approximately 8 fmoles and 4 fmoles respectively of human growth hormone (HGH) antibodies, when their enzyme activities were measured spectrophotometrically. The detection limit of the beta-galactosidase label was increased 4-fold when a fluorimetric detection system was employed.
D000818:Animals; D000906:Antibodies; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D005696:Galactosidases / Q000276:immunology*; D013006:Growth Hormone / Q000276:immunology; D006735:Horseradish Peroxidase / Q000276:immunology*; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D010527:Perissodactyla; D010544:Peroxidases / Q000276:immunology*; D011817:Rabbits; D001616:beta-Galactosidase / Q000276:immunology*
D000906:Antibodies; D007106:Immune Sera; D013006:Growth Hormone; D006735:Horseradish Peroxidase; D010544:Peroxidases; D005696:Galactosidases; D001616:beta-Galactosidase