Rapid removal to the liver of intravenously injected lipoprotein lipase.
Lipoprotein lipase was purified from bovine milk and labeled with 125I. After intravenous injection to rats the labeled lipase rapidly disappeared from the blood. The initial half-life was about 1 min and more than 70% of the radioactivity was found in the liver at 10 min. 30 min after the injection about 10% of the injected radioactivity was present in acid-soluble form in blood, indicating that the enzyme had been rapidly degraded. Injection of asialofetuin, ribonuclease B or mannan in amounts known to block the hepatic receptors for glycoproteins with exposed galactose, N-acetylglucosamine or mannose residues did not retard the removal of the lipoprotein lipase. Thus, some other, as yet undefined, receptor is implicated. Lipoprotein lipase is known to bind to heparin and some related polysacchrides. Heparin injected before the enzyme delayed its removal and heparin injected after the enzyme caused an immediate increase in blood radioactivity, signifying return from tissues to blood of labeled enzyme. Lipoprotein lipase is present at the endothelium in several extrahepatic tissues and is rapidly turned over. Its presence in blood in appreciable amounts would cause a derangement of lipid transport. The efficient hepatic removal of the enzyme may thus serve an important physiological purpose in keeping the blood levels of this enzyme low.
D000818:Animals; D001667:Binding, Competitive; D006493:Heparin / Q000494:pharmacology; D007457:Iodine Radioisotopes; D008071:Lipoprotein Lipase / Q000097:blood / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D008351:Mannans / Q000494:pharmacology; D011479:Protamines / Q000494:pharmacology; D011551:Pseudomonas fluorescens / Q000201:enzymology; D051381:Rats; D012260:Ribonucleases / Q000494:pharmacology; D000509:alpha-Fetoproteins / Q000494:pharmacology
D007457:Iodine Radioisotopes; D008351:Mannans; D011479:Protamines; D000509:alpha-Fetoproteins; D006493:Heparin; D012260:Ribonucleases; D008071:Lipoprotein Lipase