Specific labelling of replicating SPP1 DNA: analysis of viral DNA synthesis and identification of phage DNA-genes.
Specific labelling of replicating bacteriophage SPP1 DNA can be achieved by infection at nonpermissive temperature of a B. subtilis strain carrying the initation mutation dnaB ts134. Under these conditions host DNA synthesis is reduced by 90 to 95%. This technique was used to identify cistrons of SPP1 involved in phage DNA synthesis and to define intermediates in SPP1 replication.
D001412:Bacillus subtilis; D001435:Bacteriophages / Q000235:genetics*; D004261:DNA Replication; D004279:DNA, Viral / Q000235:genetics*; D005814:Genes, Viral; D005821:Genetic Techniques
D004279:DNA, Viral