The histochemical demonstration of guanase: observations in the human central nervous system.
1. A method is described for the histochemical demonstration of the purine catabolizing enzyme guanase, employing glutaraldehyde fixation and Nitro blue tetrazolium (NBT). Parallel biochemical studies confirm that enzyme activity is not significantly inhibited by exposure to glutaraldehyde. 2. By this procedure guanase activity has been visualized in neurons and glial elements of the human central nervous system (CNS). 3. Controls consisted of direct incubation of cryostat sections with a specific inhibitor of guanase (5-amino-4-imidazole carboxamide) and omission successively of the substrate guanine, of xanthine oxidase and of NBT. Enzyme activity was completely inhibited by the above procedures, and by boiling of tissues for 10 min prior to fixation. 4. Levels of enzyme activity in spinal cord and brain were assessed by a subjective scoring method, and showed close comparability with biochemical assay data in brainstem and cerebral hemispheres; whereas a low correlation for enzyme activity was observed in spinal cord and cerebellum. Differences between biochemical and histochemical assessments of CNS guanase activity are discussed.
D000619:Aminohydrolases / Q000032:analysis*; D001921:Brain / Q000201:enzymology* / Q000648:ultrastructure; D006148:Guanine Deaminase / Q000032:analysis*; D006651:Histocytochemistry; D006801:Humans; D009457:Neuroglia / Q000201:enzymology; D009474:Neurons / Q000201:enzymology; D013116:Spinal Cord / Q000201:enzymology* / Q000648:ultrastructure; D014018:Tissue Distribution
D000619:Aminohydrolases; D006148:Guanine Deaminase