title: Purification and partial characterization of three extracellular cellulases from Cellulomonas sp.
abstract: Three extracellular cellulases have been purified from cultures of Cellulomonas. One was found in solution in the cell-free supernatant and two others were found to be bound to the cellulose added as a carbon source. The free enzyme and one of the cellulose-bound enzymes bind to Sephadex. The two cellulose-bound enzymes are glycosylated. The three enzymes behave as endocellulases towards soluble carboxymethyl-cellulose and have little activity on cellulose powder.
mesh_terms: D000192:Actinomycetales / Q000201:enzymology*; D002480:Cellulase / Q000302:isolation & purification* / Q000378:metabolism; D002482:Cellulose; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006868:Hydrolysis; D007527:Isoenzymes / Q000302:isolation & purification* / Q000378:metabolism; D007700:Kinetics
chemical_list: D007527:Isoenzymes; D002482:Cellulose; D002480:Cellulase