Elimination of inter-species reactive anti-IgG antibodies by affinity chromatography.
Inter-species reactive anti-IgG antibodies are disturbing in blocking and interference experiments with the sensitive mixed hemadsorption (MH) technique. By affinity chromatography this disturbance could be efficiently eliminated. Anti-IgG sera were passed through columns containing heterologous IgG. The absorption technique is rapid and simple. After one absorption the inter-species reactivity in anti-IgG sera had been decreased thousand-fold, while any loss of species-specific reactivity could not be detected. Such absorbed anti-IgG sera reacted only with homologous antibodies. The effect is exemplified with e.g. blocking of human HLA antibodies by anti-beta2-microglobulin from rabbits.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D000941:Antigens; D002846:Chromatography, Affinity / Q000379:methods*; D005260:Female; D000882:Haplorhini; D006801:Humans; D007074:Immunoglobulin G; D011817:Rabbits; D013045:Species Specificity
D000888:Antibodies, Anti-Idiotypic; D000941:Antigens; D007074:Immunoglobulin G