Localization of 99mTc-labeled immune splenocytes at tumor site and detection by gamma camera imaging.
Better diagnostic techniques are needed to delineate the size and location of malignant primary tumors and metastatic deposits. We are developing an in vivo tumor visualization technique utilizing 99mTc-labeled immune lymphoid cells. We have investigated the in vivo organ and tissue distribution and tumor localization of 99m sodium pertechnetate, 99mTc, reduced by SnCl2 - 2H2O and 99mTc-labeled viable immune and nonimmune lymphocytes 32 hrs after injection into tumor-bearing mice. The distribution and tumor localization of the 99mTc-labeled compounds and splenocytes within tumor-bearing mice after 32 hrs was determined by imaging with a gamma camera and was quantitated by counting the various dissected organs and tissues in a gamma counter. The gamma camera images and postmortem dissection and counting showed localization of radioactivity at several sites, including the tumor in mice injected with radiolabeled immune splenocytes whereas studies utilizing other 99mTc-labeled substances or nonimmune cells showed little or no localization of radioactivity in the tumor when compared to normal leg tissue.
D000818:Animals; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D012515:Mast-Cell Sarcoma / Q000000981:diagnostic imaging* / Q000276:immunology; D051379:Mice; D008811:Mice, Inbred DBA; D009368:Neoplasm Transplantation; D011877:Radionuclide Imaging; D012513:Sarcoma, Experimental / Q000000981:diagnostic imaging / Q000276:immunology; D013154:Spleen / Q000276:immunology*; D013667:Technetium
D013667:Technetium