Utilization of exogenous purine compounds in Bacillus cereus. Translocation of the ribose moiety of inosine.
Intact cells of Bacillus cereus catalyze the breakdown of exogenous AMP to hypoxanthine and ribose 1-phosphate through the successive action of 5'-nucleotidase, adenosine deaminase, and inosine phosphorylase. Inosine hydrolase was not detectable, even in crude extracts. Inosine phosphorylase causes a "translocation" of the ribose moiety (as ribose 1-phosphate) inside the cell, while hypoxanthine remains external. Even though the equilibrium of the phosphorolytic reaction favors nucleoside synthesis, exogenous inosine (as well as adenosine and AMP) is almost quantitatively transformed into external hypoxanthine, since ribose 1-phosphate is readily metabolized inside the cell. Most likely, the translocated ribose 1-phosphate enters the sugar phosphate shunt, via its prior conversion into ribose 5-phosphate, thus supplying the energy required for the subsequent uptake of hypoxanthine in B. cereus.
D000249:Adenosine Monophosphate / Q000378:metabolism; D001409:Bacillus cereus / Q000378:metabolism*; D007042:Hypoxanthines / Q000378:metabolism; D007288:Inosine / Q000378:metabolism*; D007700:Kinetics; D011687:Purines / Q000378:metabolism*; D012266:Ribose / Q000378:metabolism*; D012267:Ribosemonophosphates / Q000378:metabolism
D007042:Hypoxanthines; D011687:Purines; D012267:Ribosemonophosphates; D000249:Adenosine Monophosphate; D007288:Inosine; D012266:Ribose