Radioimmunoassay for danazol in human and monkey plasma.
A sensitive method is described for the radioimmunoassay of danazol in monkey and human plasma. Antiserum was developed in rabbits, and a second antibody was used to separate bound from free danazol. The radioimmunoassay was specific for danazol, and the limit of detection ranged from 1.4 to 2.8 ng/ml. Exogeneous danazol could be quantitated accurately in both monkey and human plasma. The radioimmunoassay results agreed with values obtained by inverse isotope dilution after intravenous administration of 14C-danazol to monkeys. The assay was used successfully to measure danazol in plasma from human volunteers receiving 200 mg of danazol.
D000818:Animals; D003613:Danazol / Q000097:blood*; D005260:Female; D000882:Haplorhini; D006801:Humans; D008253:Macaca mulatta; D008722:Methods; D011245:Pregnadienes / Q000097:blood*; D011863:Radioimmunoassay; D011865:Radioisotope Dilution Technique
D011245:Pregnadienes; D003613:Danazol