title: Autoagglutination dispersal utilizing sulphydryl compounds.
abstract: Autoagglutination caused by powerful IgM cold autoagglutinins can be dispersed by sulphydryl compounds to provide an unagglutinated red blood cell suspension suitable for immunohematologic testing. For this 0.1 M 2-mercaptoethanol is added to twice washed red blood cells. Following agitation for 10 minutes at 37 C an unagglutinated red blood cell suspension is obtained without adverse effect to red blood cell antigens and without removal of in vivo bound IgG or complement. Dithiothreital may be substituted for 2-mercaptoethanol if the incubation time is increased to 15 minutes.
mesh_terms: D001323:Autoantibodies; D004229:Dithiothreitol / Q000494:pharmacology; D006384:Hemagglutination / Q000187:drug effects*; D006801:Humans; D007143:Immunoglobulin Heavy Chains; D007142:Immunoglobulin gamma-Chains; D008623:Mercaptoethanol / Q000494:pharmacology*; D008722:Methods
chemical_list: D001323:Autoantibodies; D007143:Immunoglobulin Heavy Chains; D007142:Immunoglobulin gamma-Chains; D008623:Mercaptoethanol; D004229:Dithiothreitol