[Rule of antibody structure: the primary structure of a human monoclonal IgA1-immunoglobulin (Myeloma protein Tro). IV. Isolation and characterization of the chymotryptic peptides of the H-chain (author's transl)].
The preceeding papers dealth with the purification and characterization of IgA immunoglobulin Tro and its H- and L-chains, the separation and characterization of the cyanogen bromide fragments and the isolation of the tryptic splitting products of the H-chain. In this paper the chymotryptic peptides of the H-chain are presented. All chymotryptic peptides were isolated and purified parallel to the isolation of tryptic peptides. On the basis of these overlapping splitting products the arrangement of the tryptic peptides in the protein could be determined. These and other splitting products listed in this paper contributed to the complete sequence determination of of the H-chain.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D002918:Chymotrypsin; D006801:Humans; D007070:Immunoglobulin A; D007143:Immunoglobulin Heavy Chains; D009101:Multiple Myeloma / Q000276:immunology; D009194:Myeloma Proteins; D010446:Peptide Fragments / Q000032:analysis
D000596:Amino Acids; D007070:Immunoglobulin A; D007143:Immunoglobulin Heavy Chains; D009194:Myeloma Proteins; D010446:Peptide Fragments; D002918:Chymotrypsin
Zur Strukturregel der Antikörper. Die Primärstruktur eines monoklonalen IgA1-Immunoglobulins (Myelomprotein Tro), IV. Isolierung und Charakterisierung der chymotryptischen Peptide der H-Kette.