Immunohistochemical demonstration of carbonic anhydrase.
Rabbits were immunized using human erythroxyte carbonic anhydrase B (HCA B) purified by the modified methods of Armstrong et al. (1966) and Bernstein and Schraer (1972). The globulin fraction was isolated by ammonium sulphate precipitation. The anti-HCA B globulin was specific, when judged using the double diffusion technique of Ouchterlony and immunoelectrophoresis. No cross reaction with human erythrocyte carbonic anhydrase C was found, but cross reactions with erythrocyte carbonic anhydrase from rat, mouse and guinea pig were observed. Flurorescein isothiocyanate conjugated goat anti-rabbit globulin was used for the localization of HCA B in tissue sections and erythrocytes on slides.
D000818:Animals; D002256:Carbonic Anhydrases / Q000276:immunology* / Q000302:isolation & purification; D002850:Chromatography, Gel; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000201:enzymology; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D006454:Hemoglobins / Q000302:isolation & purification; D006801:Humans; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D051379:Mice; D011817:Rabbits; D051381:Rats
D006454:Hemoglobins; D002256:Carbonic Anhydrases