title: Detection of herpesvirus type-specific antibody by a micro solid phase radioimmunometric assay.
abstract: A micro solid phase radioimmunometric assay has been developed which measures type-specific antibody in human sera to herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2). The test is performed by coating wells of microtiter plates with purified HSV-specified glycoproteins, VP123 and VP119, derived from HSV-1- and HSV-2-infected cells, respectively. The coated wells are then incubated with diluted unadsorbed sera and subsequently with radioiodinated goat anti-human IgG reagent. Because these major viral envelope glycoproteins contain type-specific antigenic determinants, type-specific antibodies are preferentially detected in our assay.
mesh_terms: D000914:Antibodies, Viral / Q000032:analysis*; D000918:Antibody Specificity; D003937:Diagnosis, Differential; D000939:Epitopes; D006023:Glycoproteins / Q000276:immunology; D006561:Herpes Simplex / Q000175:diagnosis; D006801:Humans; D009500:Neutralization Tests; D011863:Radioimmunoassay / Q000379:methods*; D018139:Simplexvirus / Q000276:immunology*; D014764:Viral Proteins / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D000939:Epitopes; D006023:Glycoproteins; D014764:Viral Proteins