Spontaneous development of cytotoxic activity in cultured lymphnode cells from tumour-bearing rats.
Incubation in vitro of lymphnode cells (LNC) from rats bearing a transplanted syngeneic methylcholanthrene-induced sarcoma (Mc7) resulted in the generation of a potent cytotoxic activity. Four to seven days' culture was required for development of cytotoxic activity, which was shown to be mediated by a heat-stable soluble factor. The cytotoxicity was not detectable in a 3 h or 15 h 51Cr-release assay, but was demonstrated in a 48 h microcytotoxicity assay, where post-labeling with isotopically labelled cell precursors was used to quantitate cell survival. The cytotoxicity of the cultured tumour-bearer LNC and their supernatant factor was shown to be cross-reactive for tumour cell lines other than sarcoma Mc7, and was also expressed against adult or embryonic fibroblasts.
D000818:Animals; D002478:Cells, Cultured / Q000378:metabolism; D003602:Cytotoxicity, Immunologic; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000276:immunology*; D008198:Lymph Nodes / Q000502:physiology*; D008748:Methylcholanthrene; D051381:Rats; D012513:Sarcoma, Experimental / Q000139:chemically induced / Q000276:immunology*; D004124:p-Dimethylaminoazobenzene
D008748:Methylcholanthrene; D004124:p-Dimethylaminoazobenzene