title: EDTA effect on creatine kinase (CK) and on the SCE reagent.
abstract: The inclusion of EDTA in the creatine kinase reagent recommended by the Scandinavian Committee on Enzymes was shown to increase reagent stability from less than 24 h to 5 days. Part of this effect can be explained by the fact that EDTA delays the formation of inhibitory products formed when N-acetyl cysteine is oxidized. The addition of EDTA to the reagent also results in increased measured CK activity. This effect is more pronounced for CK-BB than for CK-MM. Calcium and ferric ions are shown to inhibit the enzyme and the chelation of these ions can partly explain the observed increase of CK acitivity.
mesh_terms: D000111:Acetylcysteine; D002118:Calcium / Q000494:pharmacology; D002614:Chelating Agents; D003402:Creatine Kinase / Q000097:blood / Q000592:standards*; D004355:Drug Stability; D004492:Edetic Acid / Q000494:pharmacology*; D005290:Ferric Compounds / Q000494:pharmacology; D066298:In Vitro Techniques; D007202:Indicators and Reagents / Q000592:standards*; D007527:Isoenzymes; D008623:Mercaptoethanol; D012537:Scandinavian and Nordic Countries; D013438:Sulfhydryl Compounds; D013997:Time Factors
chemical_list: D002614:Chelating Agents; D005290:Ferric Compounds; D007202:Indicators and Reagents; D007527:Isoenzymes; D013438:Sulfhydryl Compounds; D008623:Mercaptoethanol; D004492:Edetic Acid; D003402:Creatine Kinase; D002118:Calcium; D000111:Acetylcysteine