Use of UV scanning techniques in the identification of serum constituents separated by high-performance liquid chromatography.
Serum constituents separated by the reversed-phase partition mode of high-performance liquid chromatography were identified by using a stopped-flow UV scanning technique. This method of peak identification eliminates post-chromatographic sample handling. Results from UV scanning were correlated with those obtained from absorbance ratios of reference compounds and the enzymatic peak-shift technique.
D002851:Chromatography, High Pressure Liquid; D003863:Depression / Q000097:blood; D006801:Humans; D007700:Kinetics; D011683:Purine-Nucleoside Phosphorylase / Q000097:blood; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D014503:Urate Oxidase / Q000097:blood; D014969:Xanthine Oxidase / Q000097:blood
D014969:Xanthine Oxidase; D014503:Urate Oxidase; D011683:Purine-Nucleoside Phosphorylase