Effect of certain sulphydryl compounds and dimethyl sulphoxide on potassium release by certain amines and proteins from Pseudomonas aeruginosa.
Dithiothreitol, cysteine, and mercapto-ethanol inhibit K+ release from cells of Pseudomonas aeruginosa by amines and NH4Cl incubated at pH 8.5. The inhibitions are competitive for the monamines and NH4Cl but non-competitive for the diamines and spermidine. The percent inhibition is largest for tryptamine, least for spermidine and ethylene diamine. DMSO inhibits K+ release by monamines and certain proteins but not significantly by diamines and histamine. The inhibitions are competitive.
D000588:Amines / Q000494:pharmacology*; D000643:Ammonium Chloride / Q000494:pharmacology; D003545:Cysteine / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004229:Dithiothreitol / Q000494:pharmacology; D008623:Mercaptoethanol / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism*; D011506:Proteins / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000494:pharmacology*
D000588:Amines; D011506:Proteins; D013438:Sulfhydryl Compounds; D000643:Ammonium Chloride; D008623:Mercaptoethanol; D003545:Cysteine; D011188:Potassium; D004229:Dithiothreitol; D004121:Dimethyl Sulfoxide