title: Efficacy of penicillin G, cefazolin, and gentamicin in M-K medium at 4 degrees C.
abstract: Corneal-scleral shells are routinely stored in McCarey-Kaufman (M-K) medium at 4 degrees C. Antibiotics are added to the medium to suppress bacterial growth. We examined the antibacterial activity of penicillin G potassium, 100 microgram/ml, cefazolin sodium, 100 microgram/ml, and gentamicin sulfate, 20 microgram/ml against test organisms in M-K medium at 4 degrees, 23 degrees, and 37 degrees C for periods up to 18 hours. Within the limits of the experimental conditions, none of the antibiotics used could be relied on to sterilize an inoculum of sensitive bacteria in M-K medium at 4 degrees C, the recommended storage temperature.
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D002437:Cefazolin / Q000494:pharmacology; D016039:Corneal Transplantation; D003470:Culture Media; D005839:Gentamicins / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D009926:Organ Preservation / Q000379:methods*; D010400:Penicillin G / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects; D013696:Temperature; D014021:Tissue Preservation / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D005839:Gentamicins; D002437:Cefazolin; D010400:Penicillin G