title: Displacement chromatography of simple protein mixtures, using carboxymethyldextrans.
abstract: Simple mixtures of proteins have been used as models to demonstrate that the fractionation of proteins by carboxymethyldextrans on an anion exchanger (Peterson, E.A. (1978) Anal. Biochem. 90, 767--784) is fundamentally a displacement process. Examples include the separation of two closely similar proteins, the A and B forms of beta-lactoglobulin. The use of carboxymethyldextran spacers was essential for good separation, since resolution based on protein--protein displacement was generally inadequate. The origins and effects of heterogeneity in the preparations used as spacers are discussed.
mesh_terms: D000837:Anion Exchange Resins; D002852:Chromatography, Ion Exchange / Q000379:methods; D003911:Dextrans; D007782:Lactoglobulins / Q000302:isolation & purification; D011506:Proteins / Q000302:isolation & purification*
chemical_list: D000837:Anion Exchange Resins; D003911:Dextrans; D007782:Lactoglobulins; D011506:Proteins; C014392:carboxymethyl dextran