Microbial degradation of [C14C]polystyrene and 1,3-diphenylbutane.
Microbial degradation of [beta-14C]polystyrene and 1,3-diphenylbutane, a compound structurally representing the smallest repeating unit of styrene (dimer), was investigated in soil and liquid enrichment cultures. Degradation rates in soil, as determined by 14CO2 evolution from applied [14C]polystyrene, varied from 1.5 to 3.0% for a 4-month period. Although relatively low, these percentages were 15 to 30 times greater than values previously reported. Enrichment cultures, containing 1,3-diphenylbutane as the only carbon souce, were used to determine the mechanisms of microbial oxidation of the polymer chain ends. Metabolism of 1,3-diphenylbutane appeared to involve the attack by a monooxygenease to form 2-phenyl-4-hydroxyphenylbutane followed by a further oxidation and subsequent fission of the benzene ring to yield 4-phenylvaleric acid and an unidentified 5-carbon fragment via the classic meta-fission pathway. Phenylacetic acid was probably formed from 4-phenylvaleric acid by subsequent beta-oxidation of the side chain, methyl-oxidation and decarboxylation. An initial examination of the population of microorganisms in the diphenylbutane enrichment cultures indicated that these oxidative reactions are carried out by  common soil microorganism of the genera Bacillus, Pseudomonas, Micrococcus, and Nocardia.
D001419:Bacteria / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002073:Butanes / Q000378:metabolism*; D004156:Catechol Oxidase / Q000378:metabolism; D010084:Oxidation-Reduction; D011137:Polystyrenes / Q000378:metabolism*; D012988:Soil Microbiology; D013045:Species Specificity
D002073:Butanes; D011137:Polystyrenes; D004156:Catechol Oxidase