[Effect of the contrast conditions using solutions of phosphotungstic acid on the electron microscopic image of the foot-and-mouth disease virus].
A method of negative staining of foot-and-mouth disease virus preparations permits to obtain separately positive (2% phosphotungstic acid solution, pH 3.0) and negative (2% PTA solution, pH 6.8 +/- 8.0) stainings. When a 3--4% PTA solution, pH 6.8 +/- 8.0 is used, simultaneous positive and negative staining of each virion is possible which characterizes the functional heterogeneity of the virion protein membrane in interaction with PTA anions.
D005537:Aphthovirus / Q000187:drug effects* / Q000648:ultrastructure; D008854:Microscopy, Electron / Q000379:methods*; D010772:Phosphotungstic Acid / Q000494:pharmacology*; D012996:Solutions; D013194:Staining and Labeling / Q000379:methods
D012996:Solutions; D010772:Phosphotungstic Acid
Vliianie uslovii kontrastirovaniia rastvorami fosfornovol'framovoi kisloty na élektronno-mikroskopicheskoe izobrazhenie virusa iashchura.