Cytoplasmic RNA from hen reticulocytes, mouse sarcoma 180 ascites cells, rat liver and barley embryos. Their preparation and purification by a standard procedure and characterization by polyacrylamide gel electrophoresis.
1. This paper describes a standard procedure for the preparation and purification of RNA from the post-mitochondrial supernatants of a number of eukaryotes. 2. Cytoplasmic RNA was fractionated by NaCl precipitation. The 28S (26S), 18S and 5.8S rRNA, and 9S RNA, in the NaCl insoluble fraction were separated by a two-step sucrose gradient fractionation procedure. Poly(A)-containing mRNA in hen 9S RNA was purified by affinity chromatography. The 5S rRNA and tRNA in the NaCl-soluble fraction were fractionated by gel filtration. 3. Polyacrylamide gel electrophoresis showed that the above RNA species were remarkably stable and homogeneous. Differences were found in the 26-28S rRNA, 5.8S rRNA, and 9S RNA of different eukaryotes, but other cytoplasmic RNA species were identical.
D000818:Animals; D002645:Chickens; D003593:Cytoplasm / Q000032:analysis; D002523:Edible Grain / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D001467:Hordeum / Q000235:genetics*; D008099:Liver / Q000032:analysis; D008297:Male; D051379:Mice; D012313:RNA / Q000302:isolation & purification*; D051381:Rats; D011919:Rats, Inbred Strains; D012156:Reticulocytes / Q000032:analysis*; D012510:Sarcoma 180 / Q000235:genetics*
D012313:RNA