[Simple technic for culture of locus coeruleus nucleus in the newborn mouse].
The dissecting procedure and tissue culture technique on collagen coated coverslips in Leighton tubes, of newborn Mouse nucleus locus coeruleus and the regular histofluorescence demonstration of catecholaminergic fibers in the outgrowth zone are described. This simple method allows 10 day--long experiments .
D000320:Adrenergic Fibers / Q000254:growth & development; D000818:Animals; D000831:Animals, Newborn; D002467:Cell Nucleus; D046508:Culture Techniques / Q000379:methods*; D008125:Locus Coeruleus / Q000166:cytology*; D051379:Mice; D008856:Microscopy, Fluorescence
Technique simple de culture de noyau do locus coerulus du souriceau nouveau-né.