[Influence of the sterilization technic and culture media components on the growth and dissociation of Brucella abortus strain 19 in submerged cultures].
Submerged cultures of Brucella abortus strain 19 were studied in shaking flasks. The influence of the sterilization methods and the medium composition on the bacterial yield and cellular dissociation were studied. The selected medium was as follows (amounts in g/l): casein pancreatic hydrolizated 30; yeast extract 10; glucose, 30; sodium phosphate dibasic anhydrous 3,3; sodium monobasic monohydrate 9. Cell concentration of 8 . 10(10) viable cell/ml was obtained after 48 hours when the medium components were separated and sterilized at 121 degrees C for 20 min in autoclave.
D001431:Bacteriological Techniques; D002004:Brucella Vaccine / Q000592:standards*; D002003:Brucella abortus / Q000254:growth & development* / Q000302:isolation & purification; D002458:Cell Fractionation; D003470:Culture Media; D005947:Glucose; D005990:Glycerol; D009584:Nitrogen; D010461:Peptones; D013242:Sterilization / Q000379:methods
D002004:Brucella Vaccine; D003470:Culture Media; D010461:Peptones; D005947:Glucose; D009584:Nitrogen; D005990:Glycerol
Influencia de la técnica de esterilización y de los componentes del medio de cultivo en el desarrollo y disociación de Brucella abortus cepa 19 en proceso sumergido.