Studies on regulatory functions of malic enzymes. V. Comparative studies of malic enzymes in bacteria.
Screening of four malic enzymes--NAD-linked enzyme [EC 1.1.1.38], NAD, NADP-linked enzyme [EC 1.1.1.39], NADP-linked enzyme [EC 1.1.1.40], and D-malic enzyme--was carried out with cell-free extracts of the following 16 strains of bacteria by the aid of Sepharose 6B column chromatography: 9 strains of enteric bacteria, 3 strains of Pseudomonas, Alcaligenes faecalis, Agrobacterium tumefaciens, Rhodospirillum rubrum, and Clostridium tetanomorphum. All the strains tested contained at least one malic enzyme. The NADP-linked enzyme activity was found in all the strains except C. tetanomorphum, the NAD-linked enzyme activity in 12 strains--8 strains of enteric bacteria, 2 strains of Pseudomonas, Ag. tumefaciens, and C. tetanomorphum--and D-malic enzyme activity in 4 strains--A, aerogenes (IFO 3319 and 12059), Ps. fluorescens, and R. rubrum. The NADP-linked and NAD-linked enzyme activities of two strains of Pseudomonas were not separated by the chromatography. The available evidence suggested that the NAD, NADP-linked enzyme was not present in these 16 strains. The comparative studies of molecular, enzymatic, and serological properties of the malic enzymes in these 16 strains revealed a close similarity of the same types of malic enzymes among enteric bacteria.
D000421:Alcaligenes / Q000201:enzymology; D001419:Bacteria / Q000201:enzymology*; D003013:Clostridium / Q000201:enzymology; D004926:Escherichia coli / Q000201:enzymology; D008291:Malate Dehydrogenase / Q000276:immunology / Q000302:isolation & purification / Q000502:physiology*; D008970:Molecular Weight; D011549:Pseudomonas / Q000201:enzymology; D012231:Rhizobium / Q000201:enzymology; D012247:Rhodospirillum rubrum / Q000201:enzymology; D013045:Species Specificity
D008291:Malate Dehydrogenase