Centrifugal elutriation (counterstreaming centrifugation) of cells.
Lindahl first described the separation of cells by velocity sedimentation utilizing a special technique (counterstreaming centrifugation) that was later modified slightly and renamed centrifugal elutriation. Centrifugal elutriation has been applied, with variable degrees of success, to the separation of hemopoietic cells, mouse tumor cells, testicular cells, and a variety of other specialized cells as well as cells in particular phases of the cell cycle. The capacity of the elutriator to separate large numbers of cells is its chief advantage. The purities of the separated cells have not been compared with the purities of cells separated by other methods in most cases; such comparisons would permit more sophisticated comparison of elutriation with other techniques for velocity sedimentation.
D000818:Animals; D001921:Brain / Q000166:cytology; D002453:Cell Cycle; D002460:Cell Line; D002469:Cell Separation / Q000379:methods*; D002471:Cell Transformation, Neoplastic; D002498:Centrifugation / Q000379:methods*; D004912:Erythrocytes / Q000166:cytology; D056890:Eukaryota / Q000166:cytology; D006801:Humans; D007728:Kupffer Cells / Q000166:cytology; D007962:Leukocytes / Q000166:cytology; D008297:Male; D008407:Mast Cells / Q000166:cytology; D009369:Neoplasms / Q000473:pathology; D013737:Testis / Q000166:cytology