Enzymatic excision of free hypoxanthine from polydeoxynucleotides and DNA containing deoxyinosine monophosphate residues.
A DNA glycosylase that releases free hypoxanthine by selective cleavage of dIMP residues in polydeoxynucleotides and DNA containing this nonconventional nucleotide is present in Escherichia coli cell extracts. The partly purified enzyme, termed hypoxanthine-DNA glycosylase, does not require divalent cations or phosphate for activity, and it acts more efficiently on double-stranded than on single-stranded substrates. The enzyme has properties different from either uracil-DNA glycosylase or 3-methyladenine-DNA glycosylase and is present at normal levels in E. coli mutants deficient in either of the latter two DNA glycosylases.
D001412:Bacillus subtilis; D004247:DNA; D004269:DNA, Bacterial; D003854:Deoxyribonucleotides; D004926:Escherichia coli / Q000201:enzymology; D006026:Glycoside Hydrolases / Q000302:isolation & purification / Q000378:metabolism*; D007042:Hypoxanthines; D007291:Inosine Monophosphate / Q000031:analogs & derivatives; D007292:Inosine Nucleotides / Q000031:analogs & derivatives; D011089:Polydeoxyribonucleotides; D013045:Species Specificity
D004269:DNA, Bacterial; D003854:Deoxyribonucleotides; D007042:Hypoxanthines; D007292:Inosine Nucleotides; D011089:Polydeoxyribonucleotides; D007291:Inosine Monophosphate; C017193:deoxyinosine monophosphate; D004247:DNA; D006026:Glycoside Hydrolases