title: [Biochemical mutants of Actinomyces griseus, a producer of the antibiotic grisin. The isolation of the mutants and a study of the level of grisin antibiotic synthesis].
abstract: Biochemical mutants of Actinomyces griseus producing grisin were obtained under the action of chemical mutagens. The mutants were divided into 2 groups. The mutants with impaired synthesis of amino acids of the aspartic acid family, i.e. lysine, homoserine and methionine were included into the 1st group. The 2nd group included the mutants with impaired synthesis of the other amino acids, i.e. histidine, arginine, tyrosine, phenylalanine and valine. The antibiotic production level in the biochemical mutants was studied. It was found that the level of the antibiotic production was decreased in most of the biochemical mutants. A necessity for increased fonds of lysine and arginine for biosynthesis of grisin was shown.
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D009154:Mutation / Q000187:drug effects; D012641:Selection, Genetic; D013171:Spores, Bacterial / Q000187:drug effects; D013305:Streptomyces griseus / Q000302:isolation & purification* / Q000378:metabolism; D013309:Streptothricins / Q000096:biosynthesis*; D013997:Time Factors
chemical_list: D000596:Amino Acids; D000900:Anti-Bacterial Agents; D003470:Culture Media; D013309:Streptothricins
vernacular_title: Biokhimicheskie mutanty Actinomyces griseus--produtsenta antibiotika grizina. Vydelenie mutantov i izuchenie urovnia sinteza antibiotika grizina.