Effect of vitamin A acid on the growth of keratinocytes in culture.
The effect of vitamin A acid on the culture of guinea pig ear keratinocytes was investigated. Use of this agent and treatment of the plastic dishes with collagen solution facilitated attachment and growth. Vitamin A acid elicited a wave of DNA synthesis which was studied by incorporation of tritiated thymidine and quantitation of cellular DNA, particularly during the period 24--45 h after plating. During this period, thymidine incorporation into DNA was increased ten-fold by vitamin A acid and showed a complex pattern of peaks and shoulders which may represent the S phase of synchronous keratinocytes.
D000818:Animals; D002478:Cells, Cultured; D003094:Collagen / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D000078404:Epidermal Cells; D004817:Epidermis / Q000187:drug effects* / Q000254:growth & development; D006168:Guinea Pigs; D007633:Keratins / Q000096:biosynthesis*; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors; D014212:Tretinoin / Q000494:pharmacology*
D014212:Tretinoin; D007633:Keratins; D003094:Collagen; D004247:DNA; D013936:Thymidine