Rapid micro-carbohydrate test for confirmation of Neisseria gonorrhoeae.
A rapid carbohydrate utilization procedure for the confirmation of Neisseria gonorrhoeae and identification of other Neisseria species has been developed. This method utilizes both preformed enzymes, introduced in a heavy inoculum, and enzymes formed by the microorganisms as a result of growth in a small volume of super-enriched medium. Expected carbohydrate reactions were produced by 383 clinical isolates of neisseriae and were clearly visible within 4 h of incubation. The combined use of disposable glass tubes (6 by 50 mm) and microamounts of media (0.05 ml) make this method not only rapid, but also low in cost.
D001431:Bacteriological Techniques; D050260:Carbohydrate Metabolism; D006801:Humans; D009343:Neisseria / Q000378:metabolism; D009344:Neisseria gonorrhoeae / Q000302:isolation & purification* / Q000378:metabolism; D013045:Species Specificity