title: [Rule of antibody structure. Primary structure of a human monoclonal IgAl-immunoglobulin (myeloma protein Tro). VI. Amino acid sequence of the L-chain, lambda-type, subgroup II].
abstract: The primary structure of the L-chain of an IgA1-immunoglobulin (Myeloma protein Tro) has been determined by means of cleavage with trypsin and, if necessary, with alpha-chymotrypsin. The tryptic peptides of the variable part were characterized by amino acid analysis, Dansyl-Edman degradation and cleavage with carboxypeptidase; the peptides of the constant part were identified by amino acid analyses and determination of its N- and C-terminal residues. The sequence of the remaining amino acids and the arrangement of the peptides were established in homology to known structures. The protein comprises 216 amino acids. The homology of the variable part clearly characterizes it as belonging to subgroup II of lambda-chains. In positions 27a, b and c, there are the subgroup-specific additional residues and in position 96 is the characteristic deletion. The constant part of the chain is Kern- and Oz- which indicates that it has serine in position 154 and arginine in position 191.
mesh_terms: D000595:Amino Acid Sequence; D002268:Carboxypeptidases; D006801:Humans; D007070:Immunoglobulin A; D007128:Immunoglobulin Fragments; D007147:Immunoglobulin Light Chains; D007146:Immunoglobulin lambda-Chains; D009194:Myeloma Proteins; D010446:Peptide Fragments / Q000032:analysis
chemical_list: D007070:Immunoglobulin A; D007128:Immunoglobulin Fragments; D007147:Immunoglobulin Light Chains; D007146:Immunoglobulin lambda-Chains; D009194:Myeloma Proteins; D010446:Peptide Fragments; D002268:Carboxypeptidases
vernacular_title: Zur Strukturregel der Antikörper. Die Primärstruktur eines monoklonalen IgA1-immunglobulins (Myelomprotein Tro). VI. Die Aminosäuresequenz der L-Kette, lambda-Typ, Subgruppe II.