Labelling of human and rabbit platelets with 111Indium-oxine complex.
Platelets suspended in buffer were labelled with 111Indium-xoine. In the isolation procedure the platelets and a fraction of the erythrocytes are processed simultaneously, whereby the erythrocytes serve as a supporting cushion in the centrifugation steps. Various factors which influence the labelling effectiveness were examined, and the method's utility was estimated by in vivo studies in rabbits. A platelet concentration of more than 800 x 10(9)/1 and an oxine concentration of approximately 6 micrograms/ml in a plasma-poor system (transfer of platelets from plasma to buffer without further washing steps) will yield a high labelling efficiency. If the platelet isolation yield is to be large, a slight erythrocyte-leucocyte contamination must be accepted. Our experimental results indicated that this is permissible. In vivo investigations of 5 rabbits showed in vivo recoveries of platelets from 61--74% and mean survival times from 69 h to 110 h. This supports the assessment that the method of platelet isolation and labelling is gentle.
D000818:Animals; D001792:Blood Platelets; D001803:Blood Transfusion / Q000379:methods*; D002021:Buffers; D006801:Humans; D007204:Indium; D007553:Isotope Labeling / Q000379:methods*; D015125:Oxyquinoline; D010983:Plateletpheresis / Q000379:methods*; D011817:Rabbits; D011868:Radioisotopes
D002021:Buffers; D011868:Radioisotopes; D007204:Indium; D015125:Oxyquinoline