Evidence for splicing of interrupted immunoglobulin variable and constant region sequences in nuclear RNA.
Evidence is presented that the mouse light-chain coding sequence is interrupted in a 27S nuclear RNA species, whereas the sequence is continuous in both a 13S nuclear RNA and in cytoplasmic mRNA. The discontinuity of coding regions in the 27S nuclear RNA parallels the situation found in myeloma DNA and indicates, therefore, that the removal of interruptions in the V and C regions occurs at the level of nuclear RNA.
D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology; D001666:Binding Sites, Antibody / Q000235:genetics*; D002460:Cell Line; D005796:Genes; D007127:Immunoglobulin Constant Regions / Q000235:genetics*; D007143:Immunoglobulin Heavy Chains / Q000235:genetics; D007147:Immunoglobulin Light Chains / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D007136:Immunoglobulins / Q000235:genetics*; D051379:Mice; D008970:Molecular Weight; D012332:RNA, Heterogeneous Nuclear / Q000235:genetics; D014158:Transcription, Genetic
D007127:Immunoglobulin Constant Regions; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D007135:Immunoglobulin Variable Region; D007136:Immunoglobulins; D012332:RNA, Heterogeneous Nuclear