[Determination of free intracellular proline pool in fibroblast cultures].
By using a direct, rapid, sensitive colorimetric method, free intracellular proline pool from human adult skin fibroblasts in monolayer was determined. Thus under identical experimental conditions, free intracellular proline pool was about 20 pmol proline/micrograms cellular proteins, whatever the cell lines from human adult skin fibroblasts, the passage and the time of subculture. Besides, there was a poor correlation between collagen biosynthesis in fibroblast cultures and free intracellular proline pool.
D000328:Adult; D002478:Cells, Cultured; D005347:Fibroblasts / Q000032:analysis; D006801:Humans; D011392:Proline / Q000032:analysis* / Q000302:isolation & purification; D011865:Radioisotope Dilution Technique; D012867:Skin / Q000032:analysis*; D014316:Tritium
D014316:Tritium; D011392:Proline
Détermination du fond commun de la proline libre intracellulaire dans des fibroblastes en culture.