Crossreactive mixed lymphocyte reaction determinants recognized by cloned alloreactive T cells.
We have isolated clones of alloreactive T cells by soft agar cloning of primed responder cells contained in serially restimulated, mixed lymphocyte cultures. These clones recognize unique mixed lymphocyte reaction (MLR) stimulating determinants present on (C57B/6 X A/J)F1 (B6A) spleen cells. By assaying the reactivity patterns of these clones on a panel of stimulator cells, we have identified at least three separate "specificities" of alloreactive T cell clones. Several clones exhibiting one of these three reactivity patterns have been isolated on many occasions from different experiments. Using such clones of alloreactive T cells, we have identified and are attempting to define crossreactive MLR stimulating determinants exhibited by a panel of stimulator cells. Recognition by certain clones of a shared MLR determinant(s) on B6A and DBA/2 and of the lack of this determinant(s) on B6 stimulator cells questions current concepts concerning the nature of MLR stimulating determinants and T cell recognition of alloantigens.
D000818:Animals; D002999:Clone Cells / Q000276:immunology; D003429:Cross Reactions; D000939:Epitopes; D006183:H-2 Antigens / Q000032:analysis; D007158:Immunologic Techniques; D007519:Isoantigens / Q000032:analysis; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D013601:T-Lymphocytes / Q000276:immunology*
D000939:Epitopes; D006183:H-2 Antigens; D007519:Isoantigens