[Progress in isolation technic of islets of Langerhans for transplantation].
An improved technique is described which yields up to 6,000 isolated islets of Langerhans from the pancreas of rats. The quotients for insulin/amylase and insulin/protein were estimated in vitro. For in vivo testing isologous transplantations of islets were performed. Several parameters such as total body-weight, blood sugar levels, insulin i.s., glucose-tolerance and glucose-assimilation tests demonstrated that the islets still retained their normal function despite the isolation procedure.
D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D001835:Body Weight; D003584:Cytological Techniques; D003921:Diabetes Mellitus, Experimental / Q000628:therapy*; D005069:Evaluation Studies as Topic; D005951:Glucose Tolerance Test; D007328:Insulin / Q000032:analysis; D016381:Islets of Langerhans Transplantation; D008297:Male; D008722:Methods; D051381:Rats; D011917:Rats, Inbred Lew; D014184:Transplantation, Homologous
D001786:Blood Glucose; D007328:Insulin
Fortschritte in der Isoliertechnik Langerhansscher Inseln zur Transplantation.