title: Genetic analysis of vitellogenesis in Drosophila melanogaster: the identification of a temperature-sensitive mutation affecting one of the yolk proteins.
abstract: A number of female sterile mutations on the first and third chromosomes of Drosophila melanogaster have been screened for defects in the yolk proteins using polyacrylamide gel electrophoresis. Two new mutants were identified. 6m45 accumulates all three yolk proteins (YP1, YP2 and YP3) in the haemolymph but they are all absent from the ovaries suggesting it is a yolk-protein-uptake mutant. In contrast, 1163 is a temperature-sensitive mutation with a large reduction in the quantity of YP1 in the haemolymph and ovaries at 29 degrees C. Both mutants are autonomous in ovary transplant experiments.
mesh_terms: D000818:Animals; D004331:Drosophila melanogaster / Q000235:genetics*; D004530:Egg Yolk; D005260:Female; D006458:Hemolymph / Q000378:metabolism; D007247:Infertility, Female; D009154:Mutation; D010053:Ovary / Q000378:metabolism; D014176:Protein Biosynthesis; D013696:Temperature