The occurrence of chondroitin-6-sulphate in adult human compact bone tissue.
Compact bone tissue chondroitin sulphate, previously considered to be pure chondroitin-4-sulphate, was isolated from three adult human femoral diaphyses and digested with chondroitinase ABC. Assaying these digests by means of high performance liquid chromatography we could demonstrate that 12 to 14% of the disaccharide residues were 6-sulphated. The 4/6-ratios were also studied in chondroitin sulphate fractions of different molecular size. Slightly increasing amounts of 6-sulphated disaccharides were found with increasing size of the chondroitin sulphates.
D000368:Aged; D001842:Bone and Bones / Q000032:analysis*; D002807:Chondroitin / Q000031:analogs & derivatives*; D002809:Chondroitin Sulfates / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004187:Disaccharides / Q000032:analysis; D006801:Humans; D008875:Middle Aged
D004187:Disaccharides; D002807:Chondroitin; D002809:Chondroitin Sulfates