Autoagglutination dispersal utilizing sulphydryl compounds.
Autoagglutination caused by powerful IgM cold autoagglutinins can be dispersed by sulphydryl compounds to provide an unagglutinated red blood cell suspension suitable for immunohematologic testing. For this 0.1 M 2-mercaptoethanol is added to twice washed red blood cells. Following agitation for 10 minutes at 37 C an unagglutinated red blood cell suspension is obtained without adverse effect to red blood cell antigens and without removal of in vivo bound IgG or complement. Dithiothreital may be substituted for 2-mercaptoethanol if the incubation time is increased to 15 minutes.
D001323:Autoantibodies; D004229:Dithiothreitol / Q000494:pharmacology; D006384:Hemagglutination / Q000187:drug effects*; D006801:Humans; D007143:Immunoglobulin Heavy Chains; D007142:Immunoglobulin gamma-Chains; D008623:Mercaptoethanol / Q000494:pharmacology*; D008722:Methods
D001323:Autoantibodies; D007143:Immunoglobulin Heavy Chains; D007142:Immunoglobulin gamma-Chains; D008623:Mercaptoethanol; D004229:Dithiothreitol