The efficiency of aldehyde fixation for electron microscopy: stabilization of rat brain tissue to withstand osmotic stress.
Rat brains were fixed either with glutaraldehyde (GA) or formaldehyde (FA). After 20 min or 24 h fixation the osmotic sensitivity of the tissue was tested by immersion in (a) distilled water (b) 0.15 M or (c) 0.3 M cacodylate buffer. GA-fixed material retained some sensitivity to osmotic stress after 20 min fixation but was entirely resistant after 24 h fixation. Ultrastructural preservation was good after only 20 min GA-fixation, provided that the subsequent treatment was with isotonic solutions. The fixation with FA was less efficient and slower. Dark neurons and other artifacts were commonly seen after the 20 min fixation with FA. Prolongation of the FA-fixation overnight gave markedly better preservation, but however, never equivalent to that with GA.
D000447:Aldehydes; D000818:Animals; D001921:Brain / Q000648:ultrastructure*; D004396:Coloring Agents; D005557:Formaldehyde; D005976:Glutaral; D006651:Histocytochemistry; D008297:Male; D008854:Microscopy, Electron; D051381:Rats
D000447:Aldehydes; D004396:Coloring Agents; D005557:Formaldehyde; D005976:Glutaral