Cell-mediated immune response after Brucella abortus S19 vaccination.
Cell-mediated immunity to Brucella abortus S19 vaccine was measured in young heifers by the microassay for stimulation of protein synthesis (SPS) with [3H]leucine and the skin test for delayed hypersensitivity. Brucella melitensis protein allergen and a crude B abortus S19-soluble antigen were compared in the SPS test. The SPS test was negative in 5 unvaccinated heifers and strongly positive in 3 twice-vaccinated steers. However, the SPS test was positive only in 13 of 30 S19-vaccinated heifers and the delayed hypersensitivity in 9 of 29 S19-vaccinated heifers. The 2 tests gave good agreement. Vaccination-induced residual antibody titers were partly correlated with the outcome of the tests used to measure cell-mediated immunity.
D000818:Animals; D002003:Brucella abortus / Q000276:immunology*; D002417:Cattle / Q000276:immunology*; D005260:Female; D007111:Immunity, Cellular; D008213:Lymphocyte Activation; D012882:Skin Tests; D014611:Vaccination / Q000662:veterinary*