A macrophage chemotactic factor sharing common antigenicity with immunoglobulin G from DNP-ascaris extract-induced skin lesion in guinea-pig.
A macrophage chemotactic factor (factor a) was extracted from DNP-ascaris extract-induced skin lesions (showing a maximal macrophage reaction) in guinea-pigs and then purified by gel filtration on Sephadex G-100 and chromatography using DEAE-Sephadex and CM-Sephadex. This material was a thermolabile protein (free of nucleic acid) with a molecular weight of about 150,000. It shared commom antigenicity with serum IgG, and its chemotactic acitivity was completely absorbed by anti-IgG and anti-light chain antibodies, but not by anti-BSA antibody. In contrast to leucoegresin, this material seemed active for macrophages but not for neutrophils. Less active factors b and c were thermostable and not absorbed by the antibodies described above. The ratio of chemotactic activity of factors a, b and c in the skin extracts was roughly 60:25:15.
D000818:Animals; D001200:Ascaris / Q000276:immunology; D002633:Chemotaxis; D002634:Chemotaxis, Leukocyte; D004136:Dinitrobenzenes / Q000276:immunology; D006168:Guinea Pigs; D006358:Hot Temperature; D007074:Immunoglobulin G; D008264:Macrophages / Q000276:immunology*; D008297:Male; D008970:Molecular Weight; D009504:Neutrophils / Q000276:immunology; D012867:Skin / Q000276:immunology
D004136:Dinitrobenzenes; D007074:Immunoglobulin G