Induction of laccase in Basidiomycetes: the laccase-coding messenger.
Formation of the mRNA specific for the inducible forms of laccase was evidenced in Coriolus versicolor, Pleurotus ostreatus and Pholiota mutabilis. The half-life time of these mRNAs in the fungi species studied were, respectively, 30, 37 and 24 min. Molecular weight of the newly synthesized mRNA in Pleurotus ostreatus was about 4.5X10(5), consistently with the size of the inducible laccase protein. The polysome obtained from the ferulic acid-treated mycelium, synthesized in vitro a polypeptide with the electrophoretic mobility similar to that of laccase.
D001487:Basidiomycota / Q000201:enzymology*; D004156:Catechol Oxidase / Q000096:biosynthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D004790:Enzyme Induction; D005656:Fungal Proteins / Q000096:biosynthesis; D006207:Half-Life; D008970:Molecular Weight; D012333:RNA, Messenger / Q000096:biosynthesis*
D005656:Fungal Proteins; D012333:RNA, Messenger; D004156:Catechol Oxidase