Effect of anti-HLA antisera on macrophage-T-cell interactions.
Human T lymphocytes were shown to proliferate in response to tetanus toxoid antigen only in the presence of macrophages. This response was inhibited by anti-DRw but not by anti-HLA (A and B loci) antisera added to the cultures and by pretreatment of macrophages but not of T cells with anti-DRw antisera and complement. Macrophages pulsed for 18 hr with antigen and then washed were capable of triggering T-cell proliferation. Addition of anti-DRw but not anti-HLA (A and B loci) antisera during the pulse period inhibited the macrophages' ability to trigger T-cell proliferation. The data obtained indicate that human T cells recognize and proliferate in response to antigen presented by the macrophages in association with Ia-like antigens.
D000917:Antibody Formation; D000937:Antigen-Antibody Reactions; D000954:Antigens, Surface; D005802:Genes, MHC Class II; D006680:HLA Antigens; D006801:Humans; D007518:Isoantibodies; D007519:Isoantigens; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D000954:Antigens, Surface; D006680:HLA Antigens; D007518:Isoantibodies; D007519:Isoantigens