Catabolism of adenosine 5'-monophosphate in promastigotes of Leishmania tropica.
The specific activities of enzymes participating in AMP-catabolizing reactions in promastigotes of Leishmania tropica were determined. The following sequence of reactions is suggested: AMP leads to adenosine leads to adenine leads to hypoxanthine. The initial enzyme of this sequence, AMP nucleotidase, is apparently membrane-bound. The significance of AMP catabolism with respect to adenylate energy charge and a signal transfering mechanism is discussed. Adenine deaminase has been partially purified and characterized. Several purine analogues, inter alia allopurinol, proved to be inhibitors of the adenine deaminase catalyzed reaction.
D000225:Adenine / Q000378:metabolism; D000241:Adenosine / Q000378:metabolism; D000243:Adenosine Deaminase / Q000378:metabolism; D058892:Adenosine Deaminase Inhibitors; D000249:Adenosine Monophosphate / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000818:Animals; D007042:Hypoxanthines / Q000378:metabolism; D007288:Inosine / Q000378:metabolism; D007891:Leishmania / Q000378:metabolism*; D009708:Nucleotidases / Q000378:metabolism; D011683:Purine-Nucleoside Phosphorylase / Q000378:metabolism
D058892:Adenosine Deaminase Inhibitors; D007042:Hypoxanthines; D000249:Adenosine Monophosphate; D007288:Inosine; D000255:Adenosine Triphosphate; D011683:Purine-Nucleoside Phosphorylase; D009708:Nucleotidases; D000243:Adenosine Deaminase; D000225:Adenine; D000241:Adenosine