Effect of vitamin D deficiency on sarcoplasmic reticulum function and troponin C concentration of rabbit skeletal muscle.
1. Weanling rabbits were made rachitic either by a vitamin D-deficient diet or by parenteral administration of ethane 1-hydroxy-1,1-diphosphonate (EHDP) in amounts sufficient in other species to block the formation of 1,25-dihydroxycholecalciferol [1,25-(OH)2D3]. 2. The uptake of calcium into the isolated sarcoplasmic reticulum from mixed striated quadriceps muscle, and the amount of troponin C (the calcium-binding component of the troponin complex) in relation to other proteins from the same muscle, were measured. 3. In muscle from animals made rachitic by a dietary deficiency of vitamin D, the rate of uptake of calcium by the sarcoplasmic reticulum and the troponin C concentration were both significantly less (P less than 0.02) than in control littermates. In EHDP-treated animals no significant differences from controls were found. 4. These results show that dietary deficiency of vitamin D in such animals can affect muscle physiology. Since no changes are found in animals made rachitic with EHDP, who presumably have a selective deficiency of 1,25-(OH)2D3, it is possible that the effect of vitamin D on muscle is mediated through metabolites other than 1,25-(OH)2D3 such as 25-hydroxycholecalciferol.
D000818:Animals; D001835:Body Weight; D001842:Bone and Bones / Q000378:metabolism*; D002118:Calcium / Q000097:blood; D012968:Etidronic Acid / Q000494:pharmacology; D008297:Male; D009124:Muscle Proteins / Q000378:metabolism*; D009132:Muscles / Q000378:metabolism*; D010758:Phosphorus / Q000097:blood; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000503:physiopathology*; D013977:Tibia / Q000473:pathology; D014336:Troponin / Q000378:metabolism*; D014807:Vitamin D / Q000627:therapeutic use; D014808:Vitamin D Deficiency / Q000139:chemically induced / Q000378:metabolism*
D009124:Muscle Proteins; D014336:Troponin; D014807:Vitamin D; D010758:Phosphorus; D012968:Etidronic Acid; D002118:Calcium