Cellular lysis of Streptococcus faecalis induced with triton X-100.
Lysis of exponential-phase cultures of Streptococcus faecalis ATCC 9790 was induced by exposure to both anionic (sodium dodecyl sulfate) and nonionic (Triton X-100) surfactants. Lysis in response to sodium dodecyl sulfate was effective only over a limited range of concentrations, whereas Triton X-100-induced lysis occurred over a broad range of surfactant concentrations. The data presented indicate that the bacteriolytic response of growing cells to Triton X-100: (i) was related to the ratio of surfactant to cells and not the surfactant concentration per se; (ii) required the expression of the cellular autolytic enzyme system; and (iii) was most likely due to an effect of the surfactant on components of the autolytic system that are associated with the cytoplasmic membrane. The possibility that Triton X-100 may induce cellular lysis by releasing a lipid inhibitor of the cellular autolytic enzyme is discussed.
D001426:Bacterial Proteins / Q000096:biosynthesis; D001433:Bacteriolysis; D002462:Cell Membrane / Q000187:drug effects; D002701:Chloramphenicol / Q000494:pharmacology; D013293:Enterococcus faecalis / Q000187:drug effects* / Q000201:enzymology; D006867:Hydrolases / Q000378:metabolism; D009154:Mutation; D010447:Peptide Hydrolases / Q000378:metabolism; D011092:Polyethylene Glycols / Q000494:pharmacology*; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology*; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology
D001426:Bacterial Proteins; D000644:Quaternary Ammonium Compounds; D012967:Sodium Dodecyl Sulfate; D011092:Polyethylene Glycols; D002701:Chloramphenicol; D006867:Hydrolases; D010447:Peptide Hydrolases