Increasing the resolution of polyacrylamide gel electrophoresis by varying the degree of gel crosslinking.
Resolution of polyacrylamide gel electrophoresis may be substantially improved by taking advantage of the gel sieving effects of varying concentrations of bisacrylamide crosslinker. A dilution procedure is described which permits simultaneous variation of both total acrylamide concentration and percent crosslinking within a single linear regression alaysis.
D000178:Acrylamides / Q000032:analysis*; D000429:Alcohol Oxidoreductases / Q000032:analysis; D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004331:Drosophila melanogaster / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005293:Ferritins / Q000032:analysis; D006454:Hemoglobins / Q000032:analysis; D006736:Horses / Q000033:anatomy & histology; D013154:Spleen / Q000032:analysis; D014968:Xanthine Dehydrogenase / Q000032:analysis
D000178:Acrylamides; D006454:Hemoglobins; D005293:Ferritins; D000429:Alcohol Oxidoreductases; D014968:Xanthine Dehydrogenase