[Combinations of enzyme-histochemical methods for differentiating of fibers types and evaluating the skeletal musculature (author's transl)].
This paper reports 2 combinations of enzyme-histochemical reactions (NADH tetrazolium oxidoreductase and myosin ATPase after alkaline preincubation, menadione-dependent glycerol-3-phosphate oxidoreductase and myosin ATPase after acid preincubation). One type of skeletal muscle fiber is stained golden-brown and the other blue. The differentiation of types of fibers is greatly improved by reliable classification. In addition, the same section can be used to determine the oxidative and glycolytic metabolic capacity, respectively. Finer differences in the structure of fibers may be recognized more easily and allowed of arrangement in a larger number of classes without dispensing with the old-established method of differentiating. It is possible for myopathological alterations to be made clearly visible. New and other combinations also offer promise of an advantageous extension of the method to other applications.
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism*; D006651:Histocytochemistry; D006801:Humans; D009132:Muscles / Q000166:cytology / Q000201:enzymology* / Q000473:pathology; D009218:Myosins / Q000378:metabolism*; D009246:NADH Tetrazolium Reductase / Q000378:metabolism*; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D013194:Staining and Labeling
D005993:Glycerolphosphate Dehydrogenase; D009246:NADH Tetrazolium Reductase; D009247:NADH, NADPH Oxidoreductases; D000251:Adenosine Triphosphatases; D009218:Myosins
Kombinationen enzymhistochemischer Methoden zur Fasertypendifferenzierung und Beurteilung der Skeletmuskulatur.