A separation of staphylococci and micrococci based on serological reactivity with antiserum specific for polyglycerophosphate.
A serological reaction with the antiserum against heterophile polyglycerophosphate (PGP) was evaluated for genus level differentiation among strains of Staphylococcus and Micrococcus-Sarcina spp.. Hot saline extracts from whole cells of Staphylococcus spp. strongly reacted with the PGP antiserum, whereas those of Micrococcus-Sarcina spp. did not. Likewise, phenol-water extracts from whole cells of Micrococcus-Sarcina spp. were not reactive with the PGP antiserum, although the extracts of staphylococcal cells again gave a strong reaction with the antiserum. This study indicates that extracts from Micrococcus-Sarcina spp. have no antigen reactive with the PGP antiserum and can thus be differentiated from extracts of Staphylococcus spp. which react strongly with the PGP antiserum.
D000907:Antibodies, Bacterial / Q000032:analysis; D005994:Glycerophosphates / Q000276:immunology*; D005779:Immunodiffusion; D008837:Micrococcus / Q000145:classification* / Q000276:immunology; D012505:Sarcina / Q000145:classification / Q000276:immunology; D013045:Species Specificity; D013210:Staphylococcus / Q000145:classification* / Q000276:immunology
D000907:Antibodies, Bacterial; D005994:Glycerophosphates