[Effect of EcoRI restrictase on the transforming DNA of different bacilli].
The transforming activity of DNA from Bacillus subtilis 168, Bac. subtilis W23, Bac. subtilis NRS and Bac. aterrimus after the EcoRI restrictase treatment was studied. The auxotrophic strains of Bac. subtilis 168 were used as recipients in bacterial transformation. The transforming activity for different markers decreased up to 0.001--6 per cent from the initial level for different Bacilli DNAs. In some cases the sensitivity of the same marker from different Bacilli differed in more than 50 times. Bac. subtilis and Bac. aterrimus have demonstrated the maximal differences. Such differences can be used for the identification of close related Bacilli.
D001412:Bacillus subtilis / Q000235:genetics*; D002384:Catalysis; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial; D013045:Species Specificity; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes
Deĭstvie restriktazy EcoRI na transformiruiushchuiu DNK razlichnykh batsill.