A radioimmunoassay for tallysomycin.
Antibodies were raised against a new antineoplastic agent, tallysomycin, for use in a radioimmunoassay. The assay was sensitive to 2.5 ng of tallysomycin, had a coefficient of variation of 10.5% at 50 ng/ml, and was highly specific for tallysomycin A. The antibody exhibited reduced cross-reactivity with closely related tallysomycin analogs and little or no immunoreactivity with other antitumor antibiotics including bleomycin and phleomycin. Increased immunoreactivity occurred following copper chelation of tallysomycin B as compared with the non-chelated forms, thus indicating possible conformational alterations upon metal binding.
D000903:Antibiotics, Antineoplastic / Q000276:immunology; D000918:Antibody Specificity; D001761:Bleomycin / Q000031:analogs & derivatives* / Q000032:analysis* / Q000276:immunology; D002614:Chelating Agents; D003300:Copper; D003429:Cross Reactions; D008968:Molecular Conformation; D011863:Radioimmunoassay / Q000379:methods*
D000903:Antibiotics, Antineoplastic; D002614:Chelating Agents; D001761:Bleomycin; D003300:Copper; C016126:talisomycin