In vivo sister-chromatid exchange: a sensitive measure of DNA damage.
A variety of chemical agents and X-irradiation were examined for their abilities to induce sister-chromatid exchanges (SCE) in vivo. In addition to demonstrating that several known mutagens and carcinogens are capable of inducing SCE in vivo, our studies indicate that the suspected carcinogen, tris-bromophosphate, can significantly elevate SCE levels. Comparison of the effects of these agents on SCE levels, chromosomal-aberration frequencies and cell-replication kinetics reveals that no consistent relationship exists between SCE levels and other indicators of cellular DNA damage. It is proposed that analysis of SCE induction in vivo may provide a useful technique for the screening of mutagenic and carcinogenic compounds.
D015112:4-Nitroquinoline-1-oxide / Q000494:pharmacology; D000818:Animals; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002110:Caffeine / Q000494:pharmacology; D002875:Chromosomes / Q000187:drug effects* / Q000528:radiation effects*; D003434:Crossing Over, Genetic; D003561:Cytarabine / Q000494:pharmacology; D004247:DNA / Q000235:genetics*; D003630:Daunorubicin / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D005821:Genetic Techniques; D008297:Male; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL / Q000235:genetics; D008937:Mitomycins / Q000494:pharmacology; D009153:Mutagens; D014965:X-Rays
D008937:Mitomycins; D009153:Mutagens; D003561:Cytarabine; D008769:Methylnitronitrosoguanidine; D002110:Caffeine; D015112:4-Nitroquinoline-1-oxide; D004247:DNA; D005020:Ethyl Methanesulfonate; D001973:Bromodeoxyuridine; D003630:Daunorubicin