Specific and sensitive high-performance liquid chromatographic determination of glyburide.
A specific and sensitive high-performance liquid chromatographic method has been developed for the rapid determination of intact glyburide in dog serum. With butylparaben as an internal standard, 1 ml of acid-buffered serum was extracted with toluene and an aliquot of the toluene was evaporated to dryness. The redissolved residue was chromatographed on a microparticulate reversed-phase column, and quantitation was achieved by monitoring the UV absorbance of the eluate at 228 nm. The response was linear, and the lower detection limit was approximately 20 ng/ml. Assay precision, as estimated by analyzing replicate samples of a laboratory standard, was better than 6% (CV). The utility of the analytical methodology for the determination of this highly potent sulfonylurea in pharmacokinetic studies in the dog was demonstrated.
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004285:Dogs; D005905:Glyburide / Q000032:analysis* / Q000378:metabolism; D006207:Half-Life; D007408:Intestinal Absorption; D008722:Methods; D013997:Time Factors
D005905:Glyburide