A sensitive radioimmunoassay for 6-keto-prostaglandin F1alpha: preliminary observations on circulating concentrations.
A radioimmunoassay for 6-keto-prostaglandin F1alpha (6-keto-PGF1alpha) is reported. The mean least detectable mass of 6-keto-PGF1alpha is 1.45 pg and the mean mass of added 6-keto-PGF1alpha required to displace zero-point binding by 50% is 94.2 pg. Cross-reactivities of the antiserum with all the prostaglandins and related compounds tested are less than 0.1% except for PGE2 and PGF2alpha which are respectively 0.16% and 0.80%. The assay is both accurate and reproducible and measured levels are in good agreement following extraction of 0.25--4.0 ml of plasma. Mean circulating concentrations of 6-keto-PGF1alpha in five non-pregnant humans, rhesus monkeys and sheep were 115, 65 and 25 pg/ml respectively.
D000818:Animals; D002850:Chromatography, Gel; D003429:Cross Reactions; D011464:Epoprostenol / Q000097:blood*; D005260:Female; D000882:Haplorhini; D006801:Humans; D008253:Macaca mulatta; D008297:Male; D008722:Methods; D011453:Prostaglandins / Q000097:blood*; D011460:Prostaglandins F / Q000097:blood*; D011817:Rabbits; D011863:Radioimmunoassay; D012756:Sheep
D011453:Prostaglandins; D011460:Prostaglandins F; D011464:Epoprostenol