Specific high-performance liquid chromatographic assay for nitroglycerin in dosage forms.
A specific assay for nitroglycerin dosage forms using high-performance liquid chromatography was developed. Sublingual nitroglycerin tablets are dissolved in 25 ml of water and injected directly into the chromatograph. Chromatographic conditions are: mobile phase, 60% methanol in water; flow rate, 2 ml/min; column, microparticulate reversed phase; and detection, 200 nm. The glyceryl mononitrate and dinitrate degradation products of nitroglycerin are separated from nitroglycerin and can be identified by altering the mobile phase composition of methanol.
D002851:Chromatography, High Pressure Liquid; D008722:Methods; D005996:Nitroglycerin / Q000032:analysis*; D012996:Solutions / Q000032:analysis; D013607:Tablets / Q000032:analysis
D012996:Solutions; D013607:Tablets; D005996:Nitroglycerin