Identification and characterization of a 19-S complex containing a 27 000-Mr protein in Artemia salina.
The cytoplasm of the cryptobiotic Artemia salina gastrula contains a large quantity of a unique 19-S complex. This particle is a specific aggregated form of a 27 000-Mr protein, having a molecular weight of 10(6) and an apparent buoyant density of 1.25 -- 1.26 g/cm3 in sucrose and 1.31 g/cm3 in CsCl. The relative quantity of this 19-S complex decreases significantly with respect to 80-S monoribosomes during the postgastrula development. Biochemical and immunological studies indicate that the 27 000-Mr protein is one of the RNA-binding proteins [Ovchinnikov et al., FEBS Lett. 88, 21 -- 26 (1978)] but is absent in the protein components associated with poly(A)-containing ribonucleoprotein complexes. The possibility is also suggested that the 27 000-Mr protein and Artemia elongation factor eEF-Ts might be related to each other on the basis of amino acid composition and immunological cross-reactivity.
D000478:Alkylation; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001156:Artemia / Q000032:analysis*; D002500:Centrifugation, Isopycnic; D003593:Cytoplasm / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005779:Immunodiffusion; D011506:Proteins / Q000032:analysis* / Q000302:isolation & purification; D012313:RNA / Q000378:metabolism; D012261:Ribonucleoproteins / Q000378:metabolism; D014461:Ultracentrifugation
D000596:Amino Acids; D011506:Proteins; D012261:Ribonucleoproteins; D012313:RNA