Incomplete inhibition of the immunoglobulin G-binding behavior of nitrated protein A.
Nitration of protein A with tetranitromethane incompletely blocks its Fc-binding ability for immunoglobulin G when measured by more sensitive methods of immunoprecipitation and when tested in the enzyme-linked immunosorbent assay. In addition, binding of protein A and blockage by nitration is heterogeneous and depends on the species origin of the immunoglobulin G.
D000818:Animals; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D006041:Goats; D006168:Guinea Pigs; D005779:Immunodiffusion; D007074:Immunoglobulin G; D011817:Rabbits; D013205:Staphylococcal Protein A / Q000276:immunology*; D013552:Swine
D007074:Immunoglobulin G; D013205:Staphylococcal Protein A