Establishment of a lymphoblastoid cell line and isolation of an Epstein-Barr-related virus of gorilla origin.
A B-lymphoid cell line was established from a normal gorilla. The cells contained Epstein-Barr virus-related antigens, and herpesvirus particles were demonstrated by electron microscopy. DNA-DNA reassociation kinétics revealed 30 to 40% hybridization to Epstein-Barr virus with 50 genomes per cell. Examination of the viral nuclear antigen with gorilla sera showed this to be a unique isolate termed Herpesvirus gorilla. H. gorilla transformed gibbon B-lymphocytes in vitro.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D001402:B-Lymphocytes / Q000382:microbiology; D001769:Blood / Q000382:microbiology; D002460:Cell Line; D004279:DNA, Viral / Q000032:analysis; D000939:Epitopes; D005260:Female; D006071:Gorilla gorilla / Q000382:microbiology*; D006564:Herpesviridae / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification*; D004854:Herpesvirus 4, Human / Q000032:analysis / Q000276:immunology; D006801:Humans; D008213:Lymphocyte Activation; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization
D000956:Antigens, Viral; D004279:DNA, Viral; D000939:Epitopes