Rotor speed dependent sedimentation of circular and linear DNA.
The sedimentation rate of large, linear DNA molecules has been shown to be rotor speed dependent (Rubenstein and Leighton, Biophys. Chem. 1 (1974)). In this communication we report the first studies designed to measure the rotor speed effect with a homogenous, linear viral DNA larger than bacteriophage T2 DNA. We also report the first studies using a homogenous, circular episomal DNA of known molecular weight. For this circular DNA a small rotor speed effect, previously unsuspected, was discovered.
D001412:Bacillus subtilis; D001435:Bacteriophages; D004269:DNA, Bacterial / Q000302:isolation & purification; D004270:DNA, Circular / Q000302:isolation & purification; D004279:DNA, Viral / Q000302:isolation & purification; D004926:Escherichia coli; D008433:Mathematics; D008970:Molecular Weight; D014461:Ultracentrifugation
D004269:DNA, Bacterial; D004270:DNA, Circular; D004279:DNA, Viral