title: Characterization of antigens from Aspergillus fumigatus. I. Preparation of antigens from organisms grown in completely synthetic medium.
abstract: Aspergillus fumigatus was found to grow well in completely synthetic asparagine glycerol medium. Four-day-old mycelia were totally disrupted by vigorous mechanical shaking with 0.45-micrometer glass beads. Such mechanically produced extracts contained at least 52 precipitating antigens. The number of protein and carbohydrate components was maximal in 4-day-old organisms and decreased in older cultures. Conversely, the number of components in culture filtrates increased with longer incubation periods, but was only approximately one-half that produced by the 4-day-old mycelial extracts.
mesh_terms: D000818:Animals; D000946:Antigens, Fungal / Q000032:analysis / Q000302:isolation & purification*; D001232:Aspergillus fumigatus / Q000254:growth & development / Q000276:immunology*; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D007114:Immunization; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D008722:Methods; D011817:Rabbits; D013314:Stress, Mechanical
chemical_list: D000946:Antigens, Fungal; D003470:Culture Media