[Electrophoretic and immunofluorescent study of lactate dehydrogenase isoenzymes during eye regeneration in adult tritons].
The spectrum of LDH isozymes was studied at the successive stages of retinal regeneration from the pigment epithelium and lens cells from the iris margin in the adults Pleurodeles waltlii. The combination of two methods, electrophoresis and immunofluorescence, has revealed the slow and rapid LDH isozymes with different intensity of histochemical staining in cells of the tissues under study (pigment epithelium, retina, iris and lens). During the regeneration the spectra of LDH isozymes peculiar to the pigment epithelium and iris and characterized by the predominance of slow forms were substituted by those peculiar to the retina and iris and characterized by the predominance of rapid forms. The rearrangement is realized in the proliferative phase during the transformation of one cell type into another.
D000818:Animals; D004586:Electrophoresis; D004790:Enzyme Induction; D005455:Fluorescent Antibody Technique; D005779:Immunodiffusion; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000032:analysis / Q000096:biosynthesis*; D009799:Ocular Physiological Phenomena; D012038:Regeneration; D012455:Salamandridae / Q000502:physiology*; D013997:Time Factors; D014562:Caudata / Q000502:physiology*
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase
Elektroforeticheskoe i immunofluorestsentnoe issledovanie izofermentov laktatdegidrogenazy pri regeneratsii glaza u vzroslykh tritonov.