Antibody to B. subtilis DNA polymerase III: use in enzyme purification and examination of homology among replication-specific DNA polymerases.
Bacillus subtilis DNA polymerase III (pol III), an arylhydrazinopyrimidine-sensitive, replication-specific enzyme, was used to generate a non-precipitating rabbit antibody which specifically inhibited pol III activity in vitro. The antibody was used to examine structural relationships among several DNA polymerases, and it was linked covalently to agarose; the antibody:agarose was employed to develop a rapid, selective method of purification of catalytically active B. subtilis pol III.
D000818:Animals; D000906:Antibodies; D000937:Antigen-Antibody Reactions; D001412:Bacillus subtilis / Q000201:enzymology*; D004258:DNA Polymerase III / Q000276:immunology / Q000302:isolation & purification*; D004261:DNA Replication; D004259:DNA-Directed DNA Polymerase / Q000302:isolation & purification*; D007700:Kinetics; D008970:Molecular Weight; D011817:Rabbits / Q000276:immunology; D013045:Species Specificity
D000906:Antibodies; D004258:DNA Polymerase III; D004259:DNA-Directed DNA Polymerase