Magnetically responsive polyacrylamide agarose beads for the preparation of immunoabsorbents.
Glutaraldehyde-activated magnetically responsive polyacrylamide agarose beads have been employed to bind bovine serum albumin and human, sheep and rabbit IgG. These were tested for their effectiveness as immunoabsorbents and were found to allow isolation of pure antibodies in high yields. The use of magnetically responsive beads as the solid support in immunoabsorption procedures renders isolation of antibodies easy and rapid.
D000818:Animals; D000936:Antigen-Antibody Complex / Q000302:isolation & purification*; D000937:Antigen-Antibody Reactions; D005976:Glutaral / Q000494:pharmacology; D006801:Humans; D007164:Immunosorbents / Q000302:isolation & purification*; D008280:Magnetics; D011134:Polysaccharides; D011485:Protein Binding; D011817:Rabbits; D012685:Sepharose; D012756:Sheep
D000936:Antigen-Antibody Complex; D007164:Immunosorbents; D011134:Polysaccharides; D012685:Sepharose; D005976:Glutaral