Rapid removal of acid from glycol methacrylate for improved histological embedding.
A method for the rapid and complete removal of methacrylic acid from commercial samples of glycol methacrylate is presented. It entails conversion of the acid to an insoluble N-acylurea by treatment with an equivalent amount of N,N'-dicyclohexylcarbodiimide. Sections of tissue embedded in polymer prepared from the purified monomer can be stained with basic dyes without simultaneously staining the polymer.
D000179:Acrylates; D000818:Animals; D004024:Dicyclohexylcarbodiimide / Q000494:pharmacology; D006018:Glycols; D006652:Histological Techniques; D008689:Methacrylates; D051381:Rats; D012867:Skin / Q000166:cytology; D013194:Staining and Labeling
D000179:Acrylates; D006018:Glycols; D008689:Methacrylates; D004024:Dicyclohexylcarbodiimide