Immunological and serological data concerning human histocompatibility antigens (HLA) and alpha 1-microglobulin co-purified from urine.
Serologically active glycoproteins (HLA) purified from urine contained only about 4% HLA antigens co-purified with alpha 1-microglobulin. The immunization of rabbits with the serologically active product gave rise to an antiserum containing two kinds of antibodies: anti-HLA-A9 and anti-alpha 1-microglobulin. The anti-HLA-A9 antibodies were detected by the lymphocytotoxicity and indirect immunofluorescence techniques against peripheral blood lymphocytes and cultured lymphoid cell lines. Anti-alpha 1-microblobulin antibodies were detected by immunoprecipitation in gel. Pure alpha 1-microglobulin gave rise to an antiserum directed only against alpha 1-microglobulin. This antiserum did not react with peripheral blood lymphocytes and cultured lymphoid cell lines.
D000510:Alpha-Globulins / Q000276:immunology / Q000652:urine*; D000818:Animals; D000906:Antibodies / Q000032:analysis; D003429:Cross Reactions; D003601:Cytotoxicity Tests, Immunologic; D005455:Fluorescent Antibody Technique; D006680:HLA Antigens / Q000276:immunology / Q000652:urine*; D006801:Humans; D007106:Immune Sera / Q000032:analysis; D011233:Precipitin Tests; D011817:Rabbits
D000510:Alpha-Globulins; D000906:Antibodies; D006680:HLA Antigens; D007106:Immune Sera