Receptors for activated C3 on thymus-dependent (T) lymphocytes of normal guinea-pigs.
In a survey of lymphocyte subpopulations in normal guinea-pig blood, lymph node, spleen, thymus and peritoneal cavity, a considerable overlap was observed between the percentages of C3-receptor bearing lymphocytes (CRL) and of thymus-dependent (T) cells in lymph nodes. Simultaneous rosette-formation reactions with sheep erythrocytes carrying rabbit complement (EAC) and papain-treated rabbit erythrocytes (a T-cell marker) revealed that 20--50% of the lymph node CRL were T lymphocytes. These experiments and others on cell suspensions depleted of Ig-bearing (B) lymphocytes showed that between 8 and 36% of lymph node T cells have complement receptors. The frequency of T-CRL in other lymphoid tissues was lower, representing between 0 and 8% of the T-cell population. The reaction of T-CRL and EAC was not inhibited by EDTA which is known to inhibit the C3 receptor activity on macrophages.
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects / Q000276:immunology; D003176:Complement C3; D004492:Edetic Acid / Q000494:pharmacology; D005260:Female; D006168:Guinea Pigs; D007141:Immunoglobulin Fc Fragments; D007958:Leukocyte Count; D008297:Male; D011947:Receptors, Antigen, B-Cell; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D013696:Temperature
D003176:Complement C3; D007141:Immunoglobulin Fc Fragments; D011947:Receptors, Antigen, B-Cell; D004492:Edetic Acid