Cell fusion between temperature-sensitive mutants of a Drosophila melanogaster cell line.
Temperature-sensitive (ts) mutants were isolated in a cell line of Drosophila melanogaster, GM1, by ethyl methanesulfate treatment. Two of them, ts15 and ts58, formed colonies at 23 degrees C but not at 30 degrees when inoculated at densities of/or less than 10(5) cells per 60 X 15-mm dish. By using these ts mutants, cell fusion was attempted with polyethylene glycol (PEG) 6000. Several colonies per dish developed at 30 degrees C when different ts mutants were mixed, treated with PEG, and inoculated at a density of 10(4) cells per dish. Cells in some of the colonies thus developed were propagated and their temperature-sensitive character and karyotypes were studied. The results indicated that cell fusion could be induced with PEG and that the cells which formed colonies at 30 degrees C after PEG treatment were the hybrids in which the temperature-sensitive lesions in the mutants were complemented.
D002455:Cell Division; D002459:Cell Fusion; D002460:Cell Line; D004331:Drosophila melanogaster; D005816:Genetic Complementation Test; D006822:Hybrid Cells; D007621:Karyotyping; D009154:Mutation; D011092:Polyethylene Glycols / Q000494:pharmacology; D013696:Temperature
D011092:Polyethylene Glycols