Inhibition of human seminal fluid DNA polymerase by an IgG fraction of seminal plasma from vasectomized men.
Immunoglobulin G (IgG) was isolated from ejaculates of intact and vasectomized men by precipitation with ammonium sulphate and DEAE-cellulose ionexchange chromatography. Velocity centrifugation revealed that all of the IgG from intact males was 7S protein while less than 40% of the seminal IgG of vasectomized men cosedimented with the 7S marker; the remaining, immunologically unidentifiable, protein was considerably smaller and heterogeneous in size. Only the 7S IgG from the post-vasectomy ejaculates inhibited the activity of a DNA polymerase from the seminal fluid of an intact male. These results suggest that formation of antibody reactive with the seminal fluid DNA polymerase is one manifestation of a vasectomy-associated autoimmune response in man.
D001323:Autoantibodies; D006801:Humans; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000302:isolation & purification; D008297:Male; D019384:Nucleic Acid Synthesis Inhibitors; D012661:Semen / Q000201:enzymology / Q000276:immunology*; D014659:Vasectomy
D001323:Autoantibodies; D007074:Immunoglobulin G; D019384:Nucleic Acid Synthesis Inhibitors