Activity staining for glutamate and alanine dehydrogenases in polyacrylamide gels of varying porosity. A study of bovine placental enzyme forms.
A procedure has been developed for characterizing the various molecular forms of placental and liver glutamate dehydrogenases through a combination of activity staining and varying gel pore size in electrophoresis. At a concentration of 2 mg/ml, the bovine liver GDH remained associated in a very high molecular weight form, while the placental enzyme was substantially dissociated to a molecular species of near 240,000 molecular weight and several charge isomeric species of near 160,000 molecular weight. The general approach outlined here provides a means of definitely correlating the electrophoretic behavior of various dehydrogenase isozymes with both glutamate and alanine dehydrogenase activities and molecular size and should be applicable, even in crude extracts to other dehydrogenase enzymes which exhibit multiple forms or states of association.
D000409:Alanine; D000594:Amino Acid Oxidoreductases / Q000032:analysis*; D000818:Animals; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005260:Female; D005969:Glutamate Dehydrogenase / Q000032:analysis*; D008099:Liver / Q000201:enzymology; D008970:Molecular Weight; D010920:Placenta / Q000201:enzymology; D011247:Pregnancy; D013194:Staining and Labeling / Q000379:methods
D000594:Amino Acid Oxidoreductases; D005969:Glutamate Dehydrogenase; D000409:Alanine