Renin-activation by kallikrein: evidence for alkaline-activation of plasma renin.
Porcine pancreatic kallikrein leads to a significant activation of human plasma renin when incubated at pH 8.2. 'Endogenous' activation of plasma renin can be seen by incubation of plasmas at the same pH. Both 'endogenous' alkaline- and kallikrein-activation of plasma renin can be inhibited by aprotinin. Both, kallikrein- and 'endogenous' alkaline activation of plasma renin are significantly correlated to cryo- and acid-activation. We suggest that kallikrein-and 'endogenous' alkaline-activiation come close to the in vivo activation process.
D000818:Animals; D007611:Aprotinin / Q000494:pharmacology; D003080:Cold Temperature; D004789:Enzyme Activation; D006801:Humans; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007610:Kallikreins / Q000494:pharmacology*; D010179:Pancreas / Q000201:enzymology; D012083:Renin / Q000097:blood*; D013552:Swine
D007611:Aprotinin; D007610:Kallikreins; D012083:Renin