Ultastructural localization of polysaccharides in the vacuolar system of an established cell line.
Polysaccharides in the vacuolar system of an established line of monkey kidney epithelial cells, were investigated by high resolution radioautography following incorporation of 3H-fucose and by the periodic acid-thiosemicarbazide-silver proteinate staining method. Significant amounts of glycogen and glycoproteins were found in different lysosomes and prelysosomes. The radioautographic labeling of these substances correlated with the cytochemical observations. Analysis of the 3H-fucose pattern of labeling suggests that glycoproteins are transported through the different components of the vacuolar system of the cell in a sequential fashion rather than via independent pathways. The possible functional significance of polysaccharides in lysosomes is discussed. It is suggested that glycogen is taken up by lysosomes through autophagic segregation and through infolding and vesiculation of the lysosomal surface, and that the majority of glycoprotein in the lysosomal membranes does not have acid hydrolase activity.
D000818:Animals; D002460:Cell Line; D004848:Epithelium / Q000648:ultrastructure*; D006003:Glycogen / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis; D000882:Haplorhini; D006651:Histocytochemistry; D007668:Kidney; D008247:Lysosomes / Q000032:analysis* / Q000378:metabolism; D011134:Polysaccharides / Q000032:analysis*; D014617:Vacuoles / Q000032:analysis
D006023:Glycoproteins; D011134:Polysaccharides; D006003:Glycogen