Effects of calcium-EGTA buffers on active calcium transport in inside-out red cell membrane vesicles.
In inside-out red cell membrane vesicles, the free calcium concentration half-maximally stimulating active calcium uptake is about 2 orders of magnitude smaller in a calcium-EGTA buffer than in media containing unbuffered calcium. In calcium-EGTA buffer, the maximum rate of calcium uptake is determined by the total calcium concentration present. A possible model for explaining these findings is presented.
D001693:Biological Transport, Active / Q000187:drug effects; D002118:Calcium / Q000097:blood* / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology*; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D005026:Ethylene Glycols / Q000494:pharmacology*; D006801:Humans; D007700:Kinetics
D005026:Ethylene Glycols; D004533:Egtazic Acid; D002118:Calcium