A monoclonal IgG4 (lambda) with factor V inhibitory activity.
A monoclonal IgG4 (lambda) with inhibitory activity to human coagulation factor V was isolated from the serum of a patient with a fatal hemorrhagic diathesis by using a combination of DE-52 ion exchange chromatography and isoelectric focusing techniques. Using the criteria for defining a monoclonal immunoglobulin of restricted mobility on protein electrophoresis, immunoelectrophoresis, and isoelectric focusing, as well as neutralization with class, subclass, and light chain type antisera, we are the first to demonstrate a factor V inhibitor as a monoclonal IgG4 (lambda) detectable in serum or plasma.
D002852:Chromatography, Ion Exchange; D002999:Clone Cells / Q000276:immunology; D005165:Factor V / Q000037:antagonists & inhibitors*; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D007074:Immunoglobulin G / Q000276:immunology / Q000302:isolation & purification*; D007146:Immunoglobulin lambda-Chains / Q000302:isolation & purification; D007525:Isoelectric Focusing; D009500:Neutralization Tests
D007106:Immune Sera; D007074:Immunoglobulin G; D007146:Immunoglobulin lambda-Chains; D005165:Factor V