[Existence of several non-specific recognition systems in macrophages].
Rat peritoneal cells were made to bind five particle species: immunoglobulin-coated Sheep red cells, glutaraldehyde-treated Sheep red cells, latex beads, leishmania and tumor cells. The dependence of binding on various physico-chemical parameters was studied. The binding of latex beads or Leishmania was not inhibited by cold (4 degrees C), sodium azide, cytochalasin B and ethyleneglycol or dimethylsulphoxide. The binding of immunoglobulin-coated Sheep red cells was unaffected by cold and azide, but it was inhibited by cytochalasin B, ethyleneglycol and dimethylsulphoxide. The binding of glutaraldehyde-treated Sheep red cells was inhibited by cold, azide and ethyleneglycol, but it resisted cytochalasin B and dimethylsulphoxide. The binding of tumor cells was inhibited by azide, cytochalasin B, ethyleneglycol and dimethylsulphoxide. It is concluded that: (a) macrophages are endowed several sets of non-specific binding structures that are differently affected by physico-chemical parameters, which provides a simple way of characterizing them; (b) the expression of a given binding structure on the macrophage membrane is modulated by metabolic inhibitors; (c) some lymphocytes were able to bind tumor cells or Leishmania. Thus, lymphocytes and macrophages might share some non-specific adhesive structures.
D000372:Agglutination Tests; D000818:Animals; D000963:Antimetabolites / Q000494:pharmacology; D001202:Ascitic Fluid / Q000166:cytology; D006224:Cricetinae; D004912:Erythrocytes / Q000276:immunology; D005347:Fibroblasts / Q000276:immunology; D005354:Fibrosarcoma / Q000276:immunology; D007841:Latex Fixation Tests; D007891:Leishmania / Q000276:immunology; D008264:Macrophages / Q000276:immunology*; D009374:Neoplasms, Experimental / Q000276:immunology; D051381:Rats; D012756:Sheep
D000963:Antimetabolites
Existence de plusiers systèmes de reconnaissance non spécifique chez le macrophage.