Surface expression and synthesis of I-A and I-E/C encoded molecules by B lymphocytes and Ig-secreting cells.
Splenocytes from recombinant mice were radiolabeled before or after deletion of subpopulations by cytotoxic anterisera (+C) directed against I-A, I-E/C, IgM, or Ig. Examination of the lysates of the surviving cells by immunoprecipitation demonstrated that 1) virtually all I-A and I-E/C molecules are co-expressed and synthesized by Ig+, IgM+ lymphocytes, 2) I-A, I-E/C, and IgM molecules are present on many of the cells secreting IgM and IgG, and 3) populations of Ig-bearing or Ig-secreting cells that lack detectable I-A and I-E/C antigens can be identified in spleen cell populations. The co-expression of I-A and I-E/C on most cells of the B cell lineage is discussed in terms of our present concepts of Ir gene control of immune responses.
D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology*; D000954:Antigens, Surface / Q000235:genetics* / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D003165:Complement System Proteins / Q000276:immunology; D005260:Female; D007106:Immune Sera / Q000494:pharmacology; D007072:Immunoglobulin D / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D007148:Immunoglobulin mu-Chains; D008297:Male; D051379:Mice; D011947:Receptors, Antigen, B-Cell / Q000276:immunology*; D013211:Staphylococcus aureus / Q000276:immunology
D000954:Antigens, Surface; D007106:Immune Sera; D007072:Immunoglobulin D; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007148:Immunoglobulin mu-Chains; D011947:Receptors, Antigen, B-Cell; D003165:Complement System Proteins