title: Increasing the resolution of polyacrylamide gel electrophoresis by varying the degree of gel crosslinking.
abstract: Resolution of polyacrylamide gel electrophoresis may be substantially improved by taking advantage of the gel sieving effects of varying concentrations of bisacrylamide crosslinker. A dilution procedure is described which permits simultaneous variation of both total acrylamide concentration and percent crosslinking within a single linear regression alaysis.
mesh_terms: D000178:Acrylamides / Q000032:analysis*; D000429:Alcohol Oxidoreductases / Q000032:analysis; D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004331:Drosophila melanogaster / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005293:Ferritins / Q000032:analysis; D006454:Hemoglobins / Q000032:analysis; D006736:Horses / Q000033:anatomy & histology; D013154:Spleen / Q000032:analysis; D014968:Xanthine Dehydrogenase / Q000032:analysis
chemical_list: D000178:Acrylamides; D006454:Hemoglobins; D005293:Ferritins; D000429:Alcohol Oxidoreductases; D014968:Xanthine Dehydrogenase