title: [Rule of antibody structure: the primary structure of a human monoclonal IgA1-immunoglobulin (myeloma protein Tro), I. Purification and characterization of the protein and its L- and H-chains (author's transl)].
abstract: Myeloma protein Tro was prepared from the serum of a myeloma patient by ammonium sulfate precipitation. It was purified by gel filtration and ion exchange chromatography or by Pevikon block electrophoresis. The purity of the preparation was tested by several electrophoretic or immunoelectrophoretic methods. L- and H-chains of the purified protein after reduction and alkylation were separated by gel chromatography. The protein and its L- and H-chains were characterized by amino acid analyses and end-group determinations.
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D006801:Humans; D007122:Immunoelectrophoresis; D007070:Immunoglobulin A; D007143:Immunoglobulin Heavy Chains / Q000302:isolation & purification; D007147:Immunoglobulin Light Chains / Q000302:isolation & purification; D008970:Molecular Weight; D009101:Multiple Myeloma / Q000276:immunology; D009194:Myeloma Proteins
chemical_list: D000596:Amino Acids; D007070:Immunoglobulin A; D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D009194:Myeloma Proteins
vernacular_title: Zur Strukturregel der Antikörper. Die Primärstruktur eines monoklonalen IgA1-Immunglobulins (Myelomprotein Tro), I. Reinigung und Charakterisierung des Proteins der L- und H-Ketten.