[Preparation and properties of trypsin modified by polysaccharides].
Trypsin modified by dextran preactivated with 2-amino-4,6-dichloro-1,3,5-triazine or ethyl chloroformate has been prepared. Properties of the resulting conjugates with respect to hydrolysis of synthetic substrates--methyl ester benzoyl L-arginine and n-nitrophenyl ester of n'-guanidine benzoic acid as well as high molecular weight substrates--casein and fibrin--have been studied. Stability of conjugates during autolysis and irreversible heat denaturation has been investigated.
D004789:Enzyme Activation; D005563:Formic Acid Esters / Q000737:chemistry; D006358:Hot Temperature; D006868:Hydrolysis; D011134:Polysaccharides / Q000737:chemistry*; D011489:Protein Denaturation; D013379:Substrate Specificity; D014227:Triazines / Q000737:chemistry; D014357:Trypsin / Q000138:chemical synthesis* / Q000378:metabolism
D005563:Formic Acid Esters; D011134:Polysaccharides; D014227:Triazines; C007658:ethyl chloroformate; D014357:Trypsin
Poluchenie i svoĭstva tripsina, modifitsirovannogo polisakharidami.