Polyglutaraldehyde: a new reagent for coupling proteins to microspheres and for labeling cell-surface receptors.
Glutaraldehyde polymerized in basic aqueous solutions was found to react with low molecular weight amines, immunoglobulins and hemoglobin. The polyglutaraldehyde was covalently bound to hypdrophilic microspheres. The rate of addition of proteins to the polyglutaraldehyde-derivatized microspheres was investigated spectrophotometrically as a function of pH and temperature. The reaction of polyglutaraldehyde was found to be faster than that of the monomer. The findings led to successful labeling of human lymphocyte subpopulations.
D000447:Aldehydes; D001666:Binding Sites, Antibody; D005976:Glutaral; D006454:Hemoglobins; D007141:Immunoglobulin Fc Fragments; D007202:Indicators and Reagents; D008214:Lymphocytes / Q000276:immunology; D008863:Microspheres; D008970:Molecular Weight; D011108:Polymers; D011947:Receptors, Antigen, B-Cell / Q000378:metabolism*
D000447:Aldehydes; D006454:Hemoglobins; D007141:Immunoglobulin Fc Fragments; D007202:Indicators and Reagents; D011108:Polymers; D011947:Receptors, Antigen, B-Cell; D005976:Glutaral