A cloned Drosophila DNA fragment which codes for a 4 S RNA species.
A collection of random Drosophila melanogaster DNA fragments cloned individually in Escherichia coli was screened for the presence of sequences complementary to the 4 S, 5 S and 5.8 S RNA species produced in the D. melanogaster Kc tissue culture line. Four D. melanogaster DNA fragments were found which possessed sequences complementary to the 4 S RNA species but not complementary to the 5 S or 5.8 S RNA. One such cloned fragment (6.81 kilobase in length) was characterized further. It hybridizes in situ to region 22A-C of the left arm of chromosome 2 and does not contain repetitive sequences detectable by renaturation (cot) analysis. This same region was reported earlier by Steffensen and Wimber (Genetics (1971) 69, 163--178) to hybridize in situ to bulk tRNA extracted from D. melanogaster.
D000818:Animals; D002874:Chromosome Mapping; D004274:DNA, Recombinant; D004331:Drosophila melanogaster / Q000235:genetics*; D005260:Female; D005796:Genes; D007700:Kinetics; D009693:Nucleic Acid Hybridization; D009695:Nucleic Acid Renaturation; D010957:Plasmids; D012343:RNA, Transfer / Q000235:genetics*; D014960:X Chromosome / Q000648:ultrastructure
D004274:DNA, Recombinant; D012343:RNA, Transfer