Identification of a novel RNA molecule in a new RNA processing mutant of Escherichia coli which contains 5 S rRNA sequences.
A temperature-sensitive mutant of Escherichia coli at the nonpermissive temperature fails to produce normal levels of 5 S rRNA. Instead, a number of larger RNA molecules are accumulated. One of these molecules, a 9 S RNA, contains 5 S rRNA sequences. When the strain is shifted from a nonpermissive to a permissive temperature, radioactive label is lost from the 9 S RNA and appears in 5 S rRNA. The identification of this 5 S rRNA-containing molecule indicates the participation of a new processing ribonuclease (RNase E) in the maturation of rRNA in E. coli. The 9 S RNA was not detected in a wild type strain, indicating that the processing step(s) involved in the formation of 5 S rRNA might be performed before the growing rRNA transcript is terminated.
D001483:Base Sequence; D055598:Chemical Phenomena; D002621:Chemistry; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D009841:Oligonucleotides / Q000032:analysis; D012329:RNA, Bacterial / Q000096:biosynthesis* / Q000235:genetics; D012335:RNA, Ribosomal / Q000096:biosynthesis* / Q000235:genetics; D006163:Ribonuclease T1; D013696:Temperature; D014158:Transcription, Genetic
D009841:Oligonucleotides; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D006163:Ribonuclease T1