Structure of the oligosaccharide of human J chain.
The complete structure of the oligosaccharide moiety of J chain isolated from a Waldenströms macroglobulin Wa has been established. The oligosaccharide is present in three forms differing in the amount of sialic acid and designated J-A, J-B, and J-C. The structure and proportion of each of these is: formula : (see text) : Removal of the oligosaccharide moiety with glycosidases results in an increased mobility of J chain in sodium dodecyl sulfate polyacrylamide gel electrophoresis corresponding to a shift in apparent molecular weight from 23,500 to 19,500. The preparation and utilization of iodinated glycopeptides for sequence analysis is presented.
D000596:Amino Acids / Q000032:analysis; D002241:Carbohydrates / Q000032:analysis; D006801:Humans; D007144:Immunoglobulin J-Chains; D007075:Immunoglobulin M; D008968:Molecular Conformation; D009194:Myeloma Proteins; D009844:Oligosaccharides / Q000032:analysis
D000596:Amino Acids; D002241:Carbohydrates; D007144:Immunoglobulin J-Chains; D007075:Immunoglobulin M; D009194:Myeloma Proteins; D009844:Oligosaccharides