Interaction of Mycoplasma pneumoniae with human lung fibroblasts: characterization of the in vitro model.
The interaction of pathogenic Mycoplasma pneumoniae and host cells was studied in cell cultures of MRC-5 human lung fibroblasts. A comparison of results obtained with fibroblasts in a monolayer format and with hamster tracheal explant cultures indicated that the former can bind significantly larger numbers of mycoplasmas. In addition, the attachment was 96% specific, that is, mediated through a neuraminidase-sensitive receptor on the host cell. Uptake of mycoplasmas was directly related to the number of mycoplasma cells present in the inoculum, and attachment was virtually complete within a 30-min period at 37 degrees C. High doses of M. pneumoniae induced a marked cytopathic effect, whereas doses of less than or equal to 10(6) colony-forming units per ml produced grossly observable cell damage that was moderate and variable. Transmission electron microscopy studies indicated that attachment of M. pneumoniae to the surface of lung fibroblasts occurred with the specialized terminal structure or binding site oriented closest to the epithelial cell surface. The filamentous mycoplasma cells were spatially arranged in several configurations and were not limited to a vertical orientation. The advantages and disadvantages of human lung fibroblast monolayer cultures, in reference to other in vitro models are discussed. A new mycoplasma agar medium (G-200 agar) with a defined tissue culture base and 10% horse serum is also described.
D001665:Binding Sites; D002460:Cell Line; D002462:Cell Membrane / Q000382:microbiology; D002923:Cilia / Q000648:ultrastructure; D003470:Culture Media; D005347:Fibroblasts / Q000382:microbiology*; D006801:Humans; D007700:Kinetics; D008168:Lung; D008871:Microvilli / Q000648:ultrastructure; D009177:Mycoplasma pneumoniae / Q000378:metabolism*; D014132:Trachea
D003470:Culture Media