The distribution of disulfide-groups in Descemet's membrane, lens capsule, and zonular fibers.
Zonular fibers contain large quantities of disulfide-groups which histochemically can be demonstrated after thiosulfation or reduction with sodium thioglycollate. Zonular fibers share these staining behaviours with oxytalan fibers and elastic fiber microfibrils. Smaller amounts of disulfide-groups can be demonstrated when visualized by means of the thioglycollate/ferric-ferricyanide method. Diffusion of acidic staining solutions seems to be limited in Descemet's membrane and in the lens capsule: only surfaces of these membranes become reactive after thiosulfation. It is assumed that the distribution of disulfide-groups in Descemet's membrane and in the lens capsule shows the distribution of type IV collagen.
D000818:Animals; D002415:Cats; D003094:Collagen / Q000032:analysis*; D003886:Descemet Membrane / Q000032:analysis*; D004220:Disulfides / Q000032:analysis*; D006651:Histocytochemistry; D007908:Lens, Crystalline / Q000032:analysis*; D014822:Vitreous Body / Q000032:analysis*
D004220:Disulfides; D003094:Collagen