[The isolation and purification of insulins from the pink salmon Oncorhynchus gorbuscha and the walleye pollock Theragra chalcogramma].
Crystalline insulins have been obtained from the insular tissue of the teleosts O. gorbuscha and Th. chalcogramma which is located separately from the exocrine tissue. Some tests to the homogeneity and purity of the preparations obtained were made. Biological activity of insulin as revealed by seizure tests in mice is equal to 20.1 +/- 1.19 IU/mg in O. gorbuscha and 16.9 +/- 1.99 IU/mg in Th. chalcogramma, amounting correspondingly to 83 and 70% of the activity of mammalian standard insulin. The yield of preparations calculated per wet weight of the insular tissue is equal to 2 mg/g in O. gorbuscha and 0.7 mg/g in Th. chalcogramma. It is significantly higher than the yield of mammalian insulins calculated per the weight of the pancreas. This finding may be of some interest with respect to the use of fish as a source of hormonal preparations.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D003460:Crystallization; D004591:Electrophoresis, Polyacrylamide Gel; D005399:Fishes / Q000378:metabolism*; D007328:Insulin / Q000032:analysis / Q000302:isolation & purification*; D012474:Salmon / Q000378:metabolism*
D000596:Amino Acids; D007328:Insulin
Vydelenie i ochistka insulinov gorbushi Oncorhynchus gorbuscha i mintaia Theragra chalcogramma.