[False-negative antiglobulin-(Coombs-) test caused by anticomplement bovine albumin batches in the transfusion preparation].
Different brands of bovine albumins were tested as reagent in the second step of the so called 3-stage-screening test for irregular blood group antibodies. In 3 of 9 brands of bovine albumin there was anticomplementary activity which was the cause of diminished or negative reactions in the third stage of the screening test (antiglobulin stage). The disadvantageous brands of bovine albumin contained Na2-EDTA which revealed to be responsible for the anti-complementary activity.
D001803:Blood Transfusion; D003165:Complement System Proteins; D003298:Coombs Test / Q000592:standards*; D004492:Edetic Acid / Q000494:pharmacology*; D005188:False Negative Reactions; D006801:Humans; D012710:Serum Albumin, Bovine / Q000276:immunology*
D012710:Serum Albumin, Bovine; D003165:Complement System Proteins; D004492:Edetic Acid
Falsch-negativer Antiglobulin-(Coombs-) Test durch antikomplementär wirkende Rinderalbuminchargen bei der Transfusionsvorbereitung.