[Dimethylaminochalcone, an indicator of the structural changes in the cellular envelop of E. coli under the action of Ca2+ cations and tris buffer].
An attempt was made to detect possible structural changes in E. coli cell envelope induced by Ca2+ treatment with the help of an uncharged fluorescent probe 4-dimethylaminochalcon (DMC). The effects of the treatment with tris buffer (0.01 M) at 0 degrees C and other agents (Mg2+ and EDTA) were also studied for the purpose of comparison. It is shown that Ca2+ treatment of E. coli cells results in structural changes in the cell envelope surface, whick differ from those induced by tris-buffer at 0 degrees C, Mg2+ and EDTA. DMC can be used successfully as a suitable probe for monitoring structural changes in biomembranes.
D002118:Calcium / Q000494:pharmacology*; D002413:Cations, Divalent; D002462:Cell Membrane / Q000187:drug effects; D002599:Chalcone / Q000031:analogs & derivatives; D047188:Chalcones; D004492:Edetic Acid / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects*; D005453:Fluorescence; D008274:Magnesium / Q000494:pharmacology; D011427:Propiophenones / Q000031:analogs & derivatives; D014325:Tromethamine / Q000494:pharmacology*
D002413:Cations, Divalent; D047188:Chalcones; D011427:Propiophenones; D014325:Tromethamine; C016396:dimethylaminochalcone; D002599:Chalcone; D004492:Edetic Acid; D008274:Magnesium; D002118:Calcium
Dimetilaminokhalkon--indikator strukturnykh izmeneniĭ v kletochnoĭ obolochke E. coli pod deĭstviem kationov Ca2+ i tris-bufera.