[Production and study of Bacillus subtilis mutants for genes involved in nucleoside catabolism].
By means of selection for a low thymine requirement the mutants fo thymine auxotrophs for deoxyriboaldolase (dra) and phosphodeoxyribomutase (drm) genes were obtained. Besides the mutants for pyrimidinenucleoside phosphorylase gene (pdp) were olso isolated using selection on the fluorodeoxyuridine resistance. The latter enzyme provides for pyrimidine nucleosides catabolism (thymidine, uridine) in Bacilli, as well as the conversion of exogenous thymine to thymidine in thymine auxotrophs. The data obtained when studying the deo-enzymes activities in various types of the mutants and also under the condition of induction by thymidine and acetoaldehyde are in accordance with the assumption that deoxyriboso-5-phosphate is an inductor of the deo-enzymes in Bacillus subtilis. The genes dra and pdp were tightly linked as it had been shown by the transformation experiments; in contrast, no linkage was revealed between dra and drm or pdp and drm. A secondary mutation (adn), not linked with dra and blocking the ability of bacteria to catabolise adenosine (purine nucleoside phosphorylase activity remains constant) was found in some dra-mutants.
D000079:Acetaldehyde / Q000494:pharmacology; D000446:Aldehyde-Lyases / Q000235:genetics*; D001412:Bacillus subtilis / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D004790:Enzyme Induction; D009154:Mutation; D010430:Pentosyltransferases / Q000235:genetics*; D010770:Phosphotransferases / Q000235:genetics*; D012263:Ribonucleosides / Q000378:metabolism*; D012641:Selection, Genetic; D013941:Thymine / Q000378:metabolism / Q000494:pharmacology
D012263:Ribonucleosides; D010430:Pentosyltransferases; D010770:Phosphotransferases; D000446:Aldehyde-Lyases; D000079:Acetaldehyde; D013941:Thymine
Poluchenie i issledovanie mutantov po genam katabolizma nukleozidov u Bacillus subtilis.