The use of iodinated antibody in immunodiffusion analysis to detect yeast nonsense termination fragments.
A sensitive procedure based  on the use of iodinated antibody allows the detection of antigenic material by double-diffusion analysis. An autoradiographed immunodiffusion pattern reveals distinct radio-labeled precipitation lines for an extract of yeast with wild type HIS4 protein and for extracts of mutants containing HIS4 nonsense fragments.
D000906:Antibodies; D000941:Antigens / Q000235:genetics; D005779:Immunodiffusion; D007457:Iodine Radioisotopes; D009154:Mutation; D015003:Yeasts / Q000276:immunology*
D000906:Antibodies; D000941:Antigens; D007457:Iodine Radioisotopes