Transport of Neisseria meningitidis cultures: growth-supporting media and freezing as an alternative.
The abilities of Transgrow (TG), Thayer-Martin (TM), and New York City (NYC) solid media to maintain the viability of 12 strains of Neisseria meningitidis under various controlled conditions were assessed. The effects of charcoal impregnation of swabs, temperature, and an enriched CO2 atmosphere were examined with holding for up to 21 days. Recovery from samples held at 35 degrees C was, in almost all instances, greater than at 22 or 4 degrees C. A strong requirement for added CO2 was demonstrated, especially at lower temperatures. No positive effect could be attributed to the use of charcoal-impregnated swabs. NYC and TM media were the best overall, with the former permitting recovery from more than 75% of all samples held on slants for 20 days at 4 degrees C in an atmosphere of 5% CO2 in air. Freezing, with holding on dry ice, was a useful alternative to the use of growth-supporting media. This latter method eliminated the requirement for an enriched CO2 atmosphere.
D003470:Culture Media; D005615:Freezing; D009344:Neisseria gonorrhoeae / Q000302:isolation & purification / Q000502:physiology*; D009345:Neisseria meningitidis / Q000302:isolation & purification / Q000502:physiology*; D011309:Preservation, Biological
D003470:Culture Media