Fractionation of antibodies to L-cell colony-stimulating factor by affinity chromatography.
Purified L-cell colony-stimulating factor (CSF) was coupled to cyanogen-bromide-activated Sepharose and used to selectively fractionate antibodies to this factor. With the use of a simplified two-step washing and elution technique, there was 50%--70% binding of the anti-CSF, with recovery of 60%--100% of the bound  material. Both the native antiserum and purified anti-CSF fractions were inhibitory to murine granulocyte-macrophage colony formation. The purified antibodies contained only IgG and were reduced in protein concentration to 0.1% of the serum IgG values. These fractions should prove useful tools for the study of granulocyte and macrophage differentiation.
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D005591:Chemical Fractionation; D002846:Chromatography, Affinity; D002847:Chromatography, Agarose; D003115:Colony-Stimulating Factors / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D006041:Goats; D006146:Guanidines / Q000494:pharmacology; D007106:Immune Sera / Q000494:pharmacology; D005779:Immunodiffusion; D007739:L Cells / Q000276:immunology*; D051379:Mice; D011817:Rabbits; D012756:Sheep
D000906:Antibodies; D003115:Colony-Stimulating Factors; D006146:Guanidines; D007106:Immune Sera