[Adaptation of the deoxyglucose method to the cellular level: histologic preparation of the central nervous system for high resolution radioautography].
Vascular perfusion of all products required for primary fixation, postfixation, dehydration and embedding of nervous tissue in Epon permits radio-autographic detection of radioactivity accumulated in the central nervous system after intravenous injection of [3H]deoxyglucose. This histological technique should allow application of the deoxyglucose method at cellular if not subcellular level, since a high proportion of the tracer appears to be retained in situ in specimens adequately preserved for light and electron microscope radio-autography.
D000818:Animals; D001345:Autoradiography; D002250:Carbon Radioisotopes; D002490:Central Nervous System / Q000378:metabolism*; D003837:Deoxy Sugars / Q000378:metabolism*; D003847:Deoxyglucose / Q000378:metabolism*; D006652:Histological Techniques; D008297:Male; D051381:Rats; D014316:Tritium
D002250:Carbon Radioisotopes; D003837:Deoxy Sugars; D014316:Tritium; D003847:Deoxyglucose
Adaptation de la méthode au désoxyglucose à l'échelle cellulaire: préparation histologique du système nerveux central en vue de la radio-autographie à haute résolution.