Localization of the rat epidermal SH-protease inhibitor in proventricular and vaginal squamous epithelium. An immunofluorescence study.
The rat epidermal SH-protease inhibitor was localized with the indirect immunofluorescence method in the rat proventricle and vagina fixed in cold phosphate-buffered formalin. Bright fluorescence was seen in the cytoplasms of the superficial layers of these squamous epithelia only when a highly specific antiinhibitor serum was used. The fluorescence diminished when normal rabbit serum or a rabbit antiserum againts bovine serum albumin was used instead of the anti-inhibitor serum. When the anti-inhibitor serum was neutralized with the purified epidermal inhibitor, the fluorescence was again markedly weakened. The results confirm our previous observations showing that the epidermal SH-protease inhibitor also occurs in other squamous epithelia, especially in the cytoplasms of the cells in their upper layers.
D000818:Animals; D004847:Epithelial Cells; D004848:Epithelium / Q000032:analysis; D005260:Female; D005455:Fluorescent Antibody Technique; D005753:Gastric Mucosa / Q000032:analysis / Q000166:cytology*; D011480:Protease Inhibitors / Q000032:analysis*; D051381:Rats; D013438:Sulfhydryl Compounds; D014621:Vagina / Q000032:analysis / Q000166:cytology*
D011480:Protease Inhibitors; D013438:Sulfhydryl Compounds