Role of serum factors and adherent cells in cloning of human T lymphocytes in agar culture.
In the presence of serum, 2-mercaptoethanol-treated bovine serum albumin enhances T cell colony formation as does 2-mercaptoethanol. The factor only partially substitutes for FCS, but neither for the mitogen phytohemagglutinin nor for conditioned medium derived from cultures of adherent cells.
D000362:Agar; D002448:Cell Adhesion; D002455:Cell Division; D002999:Clone Cells; D046508:Culture Techniques; D006801:Humans; D008623:Mercaptoethanol / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology
D012710:Serum Albumin, Bovine; D008623:Mercaptoethanol; D000362:Agar