The localization of tRNAAsp2 genes from Drosophila melanogaster by "in situ" hybridization.
Transfer RNAAsp2delta was isolated from Drosophila melanogaster by affinity chromatography on concanavalin A-Sepharose. The tRNA was iodinated "in vitro" with Na [125I] and hybridized "in situ" to salivary gland chromosomes from Drosophila. Subsequent autoradiography allowed the localization of the genes for tRNAAsp2 to the left arm of the second chromosome in the regions 29 D and E.
D000818:Animals; D001216:Asparagine / Q000378:metabolism*; D001345:Autoradiography; D002846:Chromatography, Affinity; D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics*; D005796:Genes; D009693:Nucleic Acid Hybridization; D012343:RNA, Transfer / Q000235:genetics*; D012469:Salivary Glands / Q000648:ultrastructure
D001216:Asparagine; D004247:DNA; D012343:RNA, Transfer