Characterization of a bacteriophage related to R-type pyocins.
A bacteriophage with a contractile tail which shows very similar features to R-type pyocins was isolated and characterized. This phage, named PS17,was purified by DEAE-cellulose chromatography and CsCl density gradient centrifugation. It was a DNA-containing phage, and the density of the purified particles in CsCl was found to be 1.468. DNA from this phage had a density of 1.720 in CsCl, indicating its guanine plus cytosine content to be 61.2%. The head was polyhedral, 69 nm in diameter, and the tail was 150 nm in length. This phage was neutralized by antiserum preparations against five R-type pyocins, and the antiserum against this phage was active in neutralizing R-type pyocins. The properties of this phage, PS17, were compared with another similar phage, PS3, which was previously reported.
D000956:Antigens, Viral / Q000032:analysis; D001430:Bacteriocins / Q000032:analysis*; D001435:Bacteriophages / Q000032:analysis* / Q000276:immunology / Q000502:physiology; D004279:DNA, Viral / Q000032:analysis; D009500:Neutralization Tests; D011550:Pseudomonas aeruginosa; D011709:Pyocins / Q000032:analysis* / Q000276:immunology; D011955:Receptors, Drug; D011991:Receptors, Virus
D000956:Antigens, Viral; D001430:Bacteriocins; D004279:DNA, Viral; D011709:Pyocins; D011955:Receptors, Drug; D011991:Receptors, Virus