Fluorometric assay for measuring biological half-life of coralyne sulfoacetate in dogs and monkeys.
A spectrophotofluorometric assay for coralyne sulfoacetate was developed. Caralyne was extracted from serum samples with n-butyl alcohol, and the drug concentrations were determined fluorometrically at 475 nm when the extract was excited at 325 nm. A biphasic serum decay curve for coralyne was observed for both dogs and monkeys. The biological half-lives for the two phases were 20 and 196 min in dogs and 15 and 142 min in monkeys.
D000818:Animals; D000970:Antineoplastic Agents / Q000097:blood*; D001600:Berberine Alkaloids / Q000097:blood*; D002855:Chromatography, Thin Layer; D004285:Dogs; D005260:Female; D006207:Half-Life; D000882:Haplorhini; D008253:Macaca mulatta; D008722:Methods; D013050:Spectrometry, Fluorescence
D000970:Antineoplastic Agents; D001600:Berberine Alkaloids; C000666:coralyne