The bacillus cereus toxin: isolation of permeability factor.
The Bacillus cereus protein has been obtained from culture fluid in homogenic form as indicated by SDS-disc electrophoresis and immunodiffusion not described before. The protein has a molecular weight of 100000 daltons. Purification was accomplished by the following steps: (1) removal of ballast nitrous components with DE-32 cellulose at pH 7.2; (2) removal of the proteins from the culture filtrate (deluted four times by water) with DE-32 cellulose at pH 8.6; (3) elution by 0.005 M tris-HCl buffer at pH 7.0 containing 0.5 M NaCl; (4) column rechromatography on DE-32 cellulose at pH 8.6. The isolated protein was identified as a vascular permeability factor acording to the bluing zone in rabbit skin tests or to the bluing lung tissue in mice.
D000818:Animals; D001409:Bacillus cereus / Q000276:immunology*; D001426:Bacterial Proteins / Q000302:isolation & purification*; D002199:Capillary Permeability; D008168:Lung / Q000187:drug effects; D051379:Mice; D009336:Necrosis; D012867:Skin / Q000187:drug effects; D014118:Toxins, Biological / Q000032:analysis* / Q000494:pharmacology
D001426:Bacterial Proteins; D014118:Toxins, Biological