Cell binding and growth inhibition by hexachlorophene of decanoate and their reversibility.
More than 80% of the hexachlorophene added to a Bacillus subtilis culture binds to the cells. Complete growth inhibition requires 6 x 10(5) molecules bound per cell. In contrast, more than 99% decanoate remains in solution and 3.8 x 10(7) molecules bound per cell are needed to inhibit growth. Centrifugation and resuspension of cells in growth medium removes only decanoate, whereas the addition of 1% bovine serum albumin to the growth medium removes both inhibitors from their binding sites on the cells. The addition of untreated cells to a hexachlorophene-treated culture enables the hexachlorophene molecules to redistribute among all the cells with the result that the inhibited cells can resume growth.
D001412:Bacillus subtilis / Q000187:drug effects* / Q000254:growth & development; D001665:Binding Sites; D003651:Decanoates / Q000494:pharmacology*; D003652:Decanoic Acids / Q000494:pharmacology*; D006582:Hexachlorophene / Q000494:pharmacology*; D012710:Serum Albumin, Bovine / Q000494:pharmacology
D003651:Decanoates; D003652:Decanoic Acids; D012710:Serum Albumin, Bovine; D006582:Hexachlorophene