Physico- and immunochemical properties of staphylococcal protein A extracellularly produced by a set of mutants from Staphylococcus aureus Cowan I.
Mutant strains of Staphylococcus aureus have been isloated using a simple cosedimentation technique. While the parental strain contains predominantly a cell-bound protein A, the mutant strains exclusively produce extracellular protein A. The mutant forms of protein A all have lower molecular weights than that of protein A from the parental strain. They showed the same antigenicity as the parental protein A and gave similar reactivity with immunoglobulin to the parental one except for one mutant. A conspicuous spur was observed between the parental protein A and that produced by the mutant against normal dog serum in the micro-Ouchterlony immunodiffusion test.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D002645:Chickens; D006801:Humans; D005779:Immunodiffusion; D008970:Molecular Weight; D009154:Mutation; D013205:Staphylococcal Protein A / Q000096:biosynthesis / Q000276:immunology / Q000302:isolation & purification; D013211:Staphylococcus aureus / Q000302:isolation & purification
D000596:Amino Acids; D013205:Staphylococcal Protein A