Transient change in the ATP pool of Anabaena cylindrica associated with ammonia assimilation.
When N2-grown cells of Anabaena cylindrica were exposed to ammonia (50 micron to 5 mM) in the dark, the size of the ATP pool was reduced by 40% within 1 min, but restored after 5 or 6 min. The decrease in ATP was accompanied by increases in ADP and AMP, while the total adenylate content remained unaltered. The ammonia-induced change in the ATP pool was completely eliminated when algal cells were treated with L-methionine-DL-sulfoximine, an inhibitor of glutamine synthesis. These results suggest that ammonia is rapidly assimilated through the pathway mediated by glutamine synthetase accompanied by reduction of the ATP pool.
D000244:Adenosine Diphosphate / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000641:Ammonia / Q000378:metabolism*; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D000458:Cyanobacteria / Q000378:metabolism*; D003624:Darkness; D008027:Light; D008717:Methionine Sulfoximine / Q000494:pharmacology
D008717:Methionine Sulfoximine; D000249:Adenosine Monophosphate; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D000244:Adenosine Diphosphate; D000641:Ammonia; D000255:Adenosine Triphosphate