Microhemagglutination test for detection of antibodies to nuclear Sm and ribonucleoprotein antigens in systemic lupus erythematosus and related diseases.
A reproducible hemagglutination procedure to detect antibodies to Sm and ribonucleoprotein nuclear antigens is described. The application and interpretation is discussed. The hemagglutination test was found to be more sensitive than the immunodiffusion test; however, the hemagglutination test may not detect the presence of low titers of anti-ribonucleoprotein in the presence of a high level of anti-Sm antibody. Anti-ribonucleoprotein antibodies in the presence of high titers of anti-Sm antibodies are best identified by gel precipitation methods where precipitin lines can be characterized by their interaction with precipitin lines produced by known prototype antisera.
D000818:Animals; D000974:Antibodies, Antinuclear / Q000032:analysis*; D001800:Blood Specimen Collection; D003584:Cytological Techniques; D004912:Erythrocytes / Q000276:immunology; D005069:Evaluation Studies as Topic; D005557:Formaldehyde; D006386:Hemagglutination Tests; D006801:Humans; D005779:Immunodiffusion; D007202:Indicators and Reagents; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D012261:Ribonucleoproteins / Q000276:immunology; D012756:Sheep; D012995:Solubility; D013949:Thymus Extracts
D000974:Antibodies, Antinuclear; D007202:Indicators and Reagents; D012261:Ribonucleoproteins; D013949:Thymus Extracts; D005557:Formaldehyde