Nuclease enhancement of specific cell agglutination in a serodiagnostic test for Neisseria gonorrhoeae.
Antiserum to a purified type R lipopolysaccharide antigen isolated from Neisseria gonorrhoeae was used in a slide agglutination test and compared with conventional carbohydrate utilization and fluorescent antibody tests to confirm the identity of laboratory cultures classified as typical or "atypical" N. gonorrhoeae. Cultures of Corynebacterium vaginalis, N. meningitidis, N. catarrhalis, N. sicca, and N. lactamicus were also tested in the slide agglutination procedure. The addition of both deoxyribonuclease and ribonuclease (1 mg/ml) to the cell suspension medium of phosphate-buffered saline improved the sensitivity and specificity of the agglutination reaction for N. gonorrhoeae. Problems relating to the agglutination test as an aid in identification of N. gonorrhoeae are discussed.
D000372:Agglutination Tests / Q000379:methods*; D000818:Animals; D050260:Carbohydrate Metabolism; D002645:Chickens / Q000276:immunology; D003851:Deoxyribonucleases / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D008070:Lipopolysaccharides / Q000276:immunology; D009344:Neisseria gonorrhoeae / Q000145:classification* / Q000276:immunology / Q000378:metabolism; D012260:Ribonucleases / Q000494:pharmacology
D007106:Immune Sera; D008070:Lipopolysaccharides; D003851:Deoxyribonucleases; D012260:Ribonucleases