The immunogloblin nature of nephritic factor (NeF).
NeF was shown to be antigenically and structurally similar to IgG by the following experiments: (1) NeF activity in serum was absorbed by and, under acid conditions, could be eluted from (a) anti-myeloma IgG antibody coupled to Sepharose and (b) protein A-Sepharose. (2) Purified NeF could bind to anit-myeloma IgG-Sepharose and could be eluted with acid, and this binding was blocked by myeloma IgG. (3) An antibody to beta2, microglobulin, showing strong cross-reactivity with normal IgG, bound NeF activity before, but not after, absorption of the antiserum with IgG. (4) Sepharose-coupled antibodies to NeF could bind activity which was recovered in the acid eluate. This binding capacity was lost after absorption of the antibody with normal and myeloma IgG. (5) Structural similarity was demonstrated by pepsin and papain digestion, which resulted in NeF activity eluting with F(ab')2 and Fab fragments from protein A-Sepharose and Sephadex G-150. (6) Autoradiography of PAGE-SDS of 125I-labelled NeF eluted from EA43bBb cells showed that NeF had a larger H chain than normal IgG, suggesting that NeF might be an abnormal IgG molecule.
D055598:Chemical Phenomena; D002621:Chemistry; D002845:Chromatography; D003178:Complement C3 Nephritic Factor; D003169:Complement Inactivator Proteins; D004591:Electrophoresis, Polyacrylamide Gel; D005779:Immunodiffusion; D007074:Immunoglobulin G; D008970:Molecular Weight; D010206:Papain; D010434:Pepsin A; D011485:Protein Binding; D013205:Staphylococcal Protein A
D003178:Complement C3 Nephritic Factor; D003169:Complement Inactivator Proteins; D007074:Immunoglobulin G; D013205:Staphylococcal Protein A; D010206:Papain; D010434:Pepsin A