Improved B cell typing for HLA-DR using nylon wool column enriched B lymphocyte preparations.
A rapid, simple procedure for preparing B lymphocytes using nylon wool has been devised. When mixtures of T lymphocytes, B lymphocytes, and monocytes are applied to nylon wool columns, B lymphocytes can then be removed, virtually free of monocytes. Such preparations (Ad) are 85 +/- 6% SIg+ and have only 3 +/- 1% monocyte contamination. The yield from peripheral blood averages 5% of all mononuclear cells. In contrast, the E rosette depletion method (E-) yields cells which are only 64 +/- 5% SIg+ and have 25 +/- 7% monocyte contamination. The superiority of the nylon method for HLA-DR typing was demonstrated in a comparative study of Ad and E- with eight normal individuals. Cytotoxic scores were higher and a large number of reactions, representing DRw groups and additional cross-reactions, was detected.
D001402:B-Lymphocytes / Q000276:immunology*; D002469:Cell Separation / Q000379:methods*; D000939:Epitopes; D006680:HLA Antigens; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D009000:Monocytes / Q000276:immunology; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000276:immunology
D000939:Epitopes; D006680:HLA Antigens