Early-blocked asporogenous mutants of Bacillus subtilis are lysogenized at reduced frequency by temperate bacteriophages.
The establishment of lysogeny in early-blocked asporogenous (Spo-) mutants of Bacillus subtilis 168, which were also defective in the production of antibiotics (Abs-), by temperate phage phi105 or SPO2 was studied. It was found that the frequency of lysogenization of Spo-Abs-mutants was 10 to 20% that of the wild-type bacteria. There was no difference in the efficiency of plating and the burst size of phi105 between wild-type and mutant strains. Phi105 lysogens of mutant strains were as stable as those of the wild type. Several rifampin-resistant mutants defective in the production of antibiotics were isolated. They were also defective in spore formation and lysogenized by phi105 at reduced frequency.
D000900:Anti-Bacterial Agents / Q000096:biosynthesis; D001412:Bacillus subtilis / Q000235:genetics / Q000502:physiology*; D001435:Bacteriophages / Q000254:growth & development*; D002874:Chromosome Mapping; D005796:Genes; D008242:Lysogeny; D009154:Mutation; D012293:Rifampin / Q000494:pharmacology; D013171:Spores, Bacterial
D000900:Anti-Bacterial Agents; D012293:Rifampin