Direct fluorescence polarization immunoassay.
Direct fluorescence polarization immunoassay of serum cortisol was established. Non-specific binding of fluorescent-labelled cortisol to serum proteins was successively eliminated by sodium dodecyl sulfate. The minimal amount of cortisol detected was 0.1 ng/tube and serum concentration of 1.0 microgram/dl to 100 microgram/dl of cortisol could be measured. This fluorescence polarization immunoassay satisfied the standard criteria of accuracy and precision. The values correlated well with those obtained by radioimmunoassay.
D005455:Fluorescent Antibody Technique; D006801:Humans; D006854:Hydrocortisone / Q000097:blood*; D010434:Pepsin A; D011863:Radioimmunoassay / Q000379:methods
D010434:Pepsin A; D006854:Hydrocortisone