Immunoperoxidase study in alpha-chain disease.
Peroxidase-antiperoxidase technique was applied to search for the presence of alpha-chain protein in cells of the infiltrate from six cases of alpha-chain disease (one immunoblastic sarcoma and five plasmacytosis cases). Cases of poorly differentiated lymphocytic and Burkitt's type lymphomas, IgA myeloma, and tuberculous enteritis served as controls. Infiltrate cells from alpha-chain disease showed heavy and diffuse staining with anti-alpha-chain and not light-chain antisera. The myeloma reacted with both anti-alpha and anti-kappa antisera. Plasma cells from tuberculous enteritis showed variable staining with anti-heavy- and anti-light-chain antisera, while control lymphomas did not stain at all. We suggest that immunoenzyme histochemistry is a useful tool in demonstrating intracellular alpha-chain protein.
D000888:Antibodies, Anti-Idiotypic; D003937:Diagnosis, Differential; D005260:Female; D006362:Heavy Chain Disease / Q000175:diagnosis*; D006801:Humans; D007124:Immunoenzyme Techniques; D007070:Immunoglobulin A / Q000032:analysis; D007143:Immunoglobulin Heavy Chains / Q000032:analysis*; D007137:Immunoglobulin alpha-Chains / Q000032:analysis*; D008297:Male
D000888:Antibodies, Anti-Idiotypic; D007070:Immunoglobulin A; D007143:Immunoglobulin Heavy Chains; D007137:Immunoglobulin alpha-Chains