False negative HLA-DR assignment necrokidney donors. A glucocorticoid effect?
Thirteen potential necrokidney donors were HLA-DR typed using peripheral blood B lymphocytes (PBBL). Antigen assignment was impossible in 10 cases because of non-reactivity of the PBBL with the HLA-DR testserum set, in some of the cases in spite of positive reactions with a positive control xenogeneic anti-human B cell antiserum. In five of these 10 donors, HLA-DR typing was also performed on B lymphocytes isolated from the spleens, and in all five cases strong specific reactions were obtained, allowing reliable antigen assignment. Evidence is provided that the negative serum reactions with the five donors' PBBL were "falsely negative". It is suggested that glucocorticoid treatment prior to blood sampling for HLA-DR typing may be responsible for the phenomenon.
D000293:Adolescent; D000328:Adult; D001402:B-Lymphocytes / Q000276:immunology; D002102:Cadaver; D000939:Epitopes; D005188:False Negative Reactions; D005260:Female; D005938:Glucocorticoids / Q000494:pharmacology*; D006680:HLA Antigens; D006650:Histocompatibility Testing; D006801:Humans; D007668:Kidney / Q000276:immunology; D016030:Kidney Transplantation; D008297:Male; D008875:Middle Aged; D013154:Spleen / Q000166:cytology; D014019:Tissue Donors; D014181:Transplantation Immunology; D014184:Transplantation, Homologous
D000939:Epitopes; D005938:Glucocorticoids; D006680:HLA Antigens