title: Studies of cultured human and simian fetal brain cells. I. Characterization of the cell types.
abstract: Explant cultures of the cerebral subventricular zone and cerebellar external germinal layer were established from fetal human, rhesus and cynomolgus monkey brains. Using comparable gestational ages, the morphogenesis of the cultures from these three sources was almost indistinguishable. Four cell types were distinguished by electron microscopy. Germinal cells or neuroblasts were confined largely to the primary explant and extended into a transitional outgrowth region. Astrocytes, which stained for glial fibrillary acidic protein, grew out of the explants and these could be distinguished from the large, mesenchymal epithelioid cells. A fourth cell type, not identified in previous studies, had the ultrastructural characteristics of an oligodendrocyte, but did not produce myelin in these culture conditions.
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology; D001921:Brain / Q000166:cytology / Q000196:embryology*; D002478:Cells, Cultured; D002531:Cerebellum / Q000196:embryology; D002540:Cerebral Cortex / Q000196:embryology; D000882:Haplorhini; D006801:Humans; D008252:Macaca fascicularis; D008253:Macaca mulatta; D008854:Microscopy, Electron; D009024:Morphogenesis; D009474:Neurons / Q000166:cytology; D009836:Oligodendroglia / Q000166:cytology; D013045:Species Specificity