[Application of hydrophobic chromatography to the fractionation of wheat prolamins].
Hydrophobic chromatography is applied to the fractionation of wheat prolamins. Proteins are separated on "Phenyl Sepharose CL 4B" column. They are eluted by variations of pH and polarity of solvent. Components with the same electrophoretic mobility appear in several chromatographic fractions and gliadin groups, as indicated by Woychick classification, are heterogeneous. This method is excepted to give new information about interaction properties of gluten proteins.
D000596:Amino Acids / Q000032:analysis; D005591:Chemical Fractionation / Q000379:methods; D002846:Chromatography, Affinity / Q000379:methods; D005903:Gliadin; D010940:Plant Proteins; D014908:Triticum / Q000032:analysis; D014783:Viscosity
D000596:Amino Acids; D010940:Plant Proteins; D005903:Gliadin
Application de la chromatographie d'interaction hydrophobe au fractionnement des prolamines du blé tendre.