[Reiterant contaminations by "pseudomonas aeruginosa" on culture cell lines in a hospital virology service (author's transl)].
The selection of antibiotic resistant Pseudomonas aeruginosa strains by an indiscriminate administration of antibiotics has been observed. The consequences induced by Ps. aeruginosa on the tissue culture cell lines (both primary and continuos) are harmful, velding a sharp pH drop from a slight initial increase, followed by the destruction of the cell layer. Sometimes the cells succeed in growing up for some passages since antibiotics are usually present in the growth medium. Tissue culture contamination can be avoided by carrying out drastic disinfection treatments. A profilatic measure would amply to keep the Virology laboratories separated from the rest of the hospital in which Ps. aeruginosa is often present.
D002353:Carrier State / Q000453:epidemiology; D002460:Cell Line; D046508:Culture Techniques; D006748:Hospital Departments; D006801:Humans; D007558:Italy; D010564:Personnel, Hospital; D011552:Pseudomonas Infections / Q000453:epidemiology; D011550:Pseudomonas aeruginosa; D014773:Virology
Inquinamenti reiteranti da "Pseudomonas aeruginosa" in linee cellulari coltivate in un servizio di virologia ospedaliero.