Characteristics of PR5, a lipid-containing plasmid-dependent phage.
An extensive characterization of plasmid-dependent phage PR5 isolated from sewage has been carried out. The phage has a head diameter of 65--68 nm, is isometric with a double-layered capsid, and a minority possess tails. It adsorbs to many but not all types of bacteria possessing P, N, or W plasmids. The phage contains 20% lipid, 15.1% DNA, and 64.9% protein by weight and has a buoyant density of 1.265 g/ml in CsCl. The DNA is double-stranded with a G + C content of 49% and a molecular weight of 7.4 +/- 0.6 x 10 (6) as shown by electron microscopy. Phospholipid content is 66% of lipid and consists of cardiolipin (13%), phosphatidylethanolamine (43%), and phosphatidylglycerol (44%) and differ quantitatively from that of host bacteria. Anti-PR5 serum inactivates other similar phages, PR3 and PR4. Phage adsorption is impaired in deep rough mutants of Salmonella minnesota.
D001435:Bacteriophages / Q000254:growth & development / Q000648:ultrastructure; D003227:Conjugation, Genetic; D004279:DNA, Viral / Q000032:analysis; D004926:Escherichia coli / Q000235:genetics; D008055:Lipids / Q000032:analysis; D008242:Lysogeny; D010957:Plasmids; D011550:Pseudomonas aeruginosa / Q000235:genetics; D012475:Salmonella / Q000235:genetics; D012722:Sewage; D014764:Viral Proteins / Q000032:analysis; D014871:Water Microbiology
D004279:DNA, Viral; D008055:Lipids; D012722:Sewage; D014764:Viral Proteins