Alteration of cartilage glycosaminoglycan protein acceptor by somatomedin and cortisol.
The effect of somatomedin and cortisol on embryonic chick cartilage in vitro indicates that somatomedin stimulates 35SO4 uptake while cortisol decreases it with no effect on glycosaminoglycan turnover. Xylosyltransferase activity is increased in crude fractions of somatomedin-treated cartilage but decreased in cortisol-treated cartilage. By using a Smith-degraded proteoglycan as an exogenous acceptor, xylosyltransferase activities from both treatments were equivalent, suggesting that the enzyme was not rate limiting. The results of xylosyltransferase assays conducted by mixing enzyme and endogenous acceptor from control, cortisol-treated and somatomedin-treated cartilage, suggest both effects to be at the level of the acceptor protein.
D000818:Animals; D002356:Cartilage / Q000187:drug effects* / Q000378:metabolism; D002642:Chick Embryo; D005700:Galactosyltransferases / Q000378:metabolism; D014453:Glucuronosyltransferase / Q000378:metabolism; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D006854:Hydrocortisone / Q000494:pharmacology*; D010430:Pentosyltransferases / Q000378:metabolism; D011509:Proteoglycans / Q000378:metabolism; D013002:Somatomedins / Q000494:pharmacology*; D014531:Uridine Diphosphate Galactose; D014535:Uridine Diphosphate Glucuronic Acid; D014540:Uridine Diphosphate Xylose; D000097608:UDP Xylose-Protein Xylosyltransferase
D005700:Galactosyltransferases; D014453:Glucuronosyltransferase; D006025:Glycosaminoglycans; D006854:Hydrocortisone; D010430:Pentosyltransferases; D011509:Proteoglycans; D013002:Somatomedins; D014531:Uridine Diphosphate Galactose; D014535:Uridine Diphosphate Glucuronic Acid; D014540:Uridine Diphosphate Xylose; D000097608:UDP Xylose-Protein Xylosyltransferase