Effect of storage conditions of serum lipoproteins on electrophoretic mobility in agarose gel and on lipid composition.
The effect of conditions of storage of sera and lipoprotein fractions on electrophoretic mobility in argarose gel and on lipid composition has been assessed. It was found that sera or lipoprotein fractions may be stored at 4 degrees C in the presence of ethylene diamine tetra-acetate for up to 5 days before electrophoresis or up to 8 days before estimation of cholesterol and triglycerides. Addition of mercurothiolate of p-chloromercuriphenyl sulphonate offerred no further advantage.
D001793:Blood Preservation; D003080:Cold Temperature; D004492:Edetic Acid; D004587:Electrophoresis, Agar Gel; D006801:Humans; D008055:Lipids / Q000032:analysis; D008074:Lipoproteins / Q000097:blood* / Q000302:isolation & purification; D013997:Time Factors
D008055:Lipids; D008074:Lipoproteins; D004492:Edetic Acid