Amplification of template capacity of mammalian DNA following extraction of low molecular weight peptides.
Experiments were carried out to evaluate the physical and functional modifications of DNA from several mammalian tissues following alkaline extraction (pH 9.5). The results indicate that such extraction provokes removal of firmly bound low molecular weight non-histone peptides involved in a general mechanism regulating the template capacity of DNA.
D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002847:Chromatography, Agarose; D002852:Chromatography, Ion Exchange; D004247:DNA / Q000032:analysis / Q000302:isolation & purification; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis / Q000302:isolation & purification*; D012313:RNA / Q000096:biosynthesis; D014018:Tissue Distribution
D010455:Peptides; D012313:RNA; D004247:DNA; D012321:DNA-Directed RNA Polymerases