Immunological purification of sea urchin egg tropomyosin.
The antiserum against lantern muscle tropomyosin of the sea urchin was prepared, and the presence of tropomyosin in the sea urchin egg was shown by immunodiffusion test between the antiserum and the egg tropomyosin fraction which was prepared according to the purification method for muscle tropomyosin. The sea urchin egg tropomyosin was isolated from the immuno-precipitate formed between the antiserum and the egg tropomyosin fraction. The subunit molecular weight of the egg tropomyosin was calculated to be 29,000.
D000818:Animals; D005260:Female; D007106:Immune Sera; D005779:Immunodiffusion; D008970:Molecular Weight; D010063:Ovum / Q000032:analysis*; D012617:Sea Urchins; D014335:Tropomyosin / Q000276:immunology / Q000302:isolation & purification*
D007106:Immune Sera; D014335:Tropomyosin