Pseudouridylation of yeast ribosomal precursor RNA.
The pseudouridylation of ribosomal RNA of Saccharomyces carlsbergensis was investigated with respect to its timing during the maturation of rRNA and its sequence specificity. Analysis of 37-S RNA, the common precursor to 17-S, 5.8-S and 26-S rRNA and most probably the primary ribosomal transcript, shows that this RNA molecule contains already most if not all of the 36-37 pseudouridine residues found in the mature rRNAs. Thus pseudouridylation is, like 2'-0-ribosemethylation, an early event in the maturation of rRNA, taking place immediately after, or even during, transcription. The data presented show that the non-conserved sequences of 37-S precursor rRNA contain very few pseudouridine residues if any. The pseudouridine residues within the rRNA sequences are apparently clustered to a certain degree as can inferred from the occurrence of a single oligonucleotide containing 3 pseudouridines, which was obtained by digestion of 26-S rRNA with ribonuclease T1.
D001483:Base Sequence; D008970:Molecular Weight; D009843:Oligoribonucleotides / Q000032:analysis; D011560:Pseudouridine / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000096:biosynthesis*; D006163:Ribonuclease T1; D012440:Saccharomyces / Q000378:metabolism*; D014158:Transcription, Genetic; D014529:Uridine / Q000031:analogs & derivatives*
D009843:Oligoribonucleotides; D012335:RNA, Ribosomal; D011560:Pseudouridine; D006163:Ribonuclease T1; D014529:Uridine