[Indirect hemagglutination test at the ultrastructural level].
A study on ultra-thin sections was made of the preparations of agglutinate produced during the reaction of the immunoglobulin erythrocytic diagnostic agent with dry corpuscular Rickettsia prowazeki antigen, fluoresceine isothiocyanate labeled, and also SRBC used for the preparation of the diagnostic agent after formalinization, tannin treatment, sensitization with hyperimmune horse serum immunoglobulins and lyophilization, respectively. Formalin and tannin treatment of erythrocytes failed to be reflected on the ultrastructure of their cellular membranes; the treatment with hemosensitin was accompanied by the appearance of spheroid protrusions of the erythrocyte cytoplasmic membrane with the preservation of its three-layer structure. Specific interaction of sensitized erythrocytes with the antigen corpuscles was expressed morphologically in their apposition or connection through a gap of 20--30 nm.
D000818:Animals; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000648:ultrastructure; D004912:Erythrocytes / Q000276:immunology*; D005557:Formaldehyde / Q000494:pharmacology; D005612:Freeze Drying; D006384:Hemagglutination; D006386:Hemagglutination Tests; D006736:Horses / Q000276:immunology; D008854:Microscopy, Electron; D012283:Rickettsia prowazekii / Q000276:immunology*; D013634:Tannins
D013634:Tannins; D005557:Formaldehyde
Issledovanie reaktsii nepriamoĭ gemaggliutinatsii na urovne ul'trastruktur.