[In vitro study on the problem of optimal gentamicin therapy in leukopenic patients: short injections or parenteral infusions?].
Killing curves of Pseudomonas aeruginosa were performed in an in vitro system simulating in-vivo gentamicin kinetics, i.e. decay of the antibiotic concentration with a 2.1 h halflife time. Minimal inhibitory concentrations (MIC) of gentamicin killed 99.999% of the initial Pseudomonas inoculum whereas 99.99% were killed at a continuously falling gentamicin concentration (starting from the MIC level) in the same period of time. Regrowth of persistent germs occurred only after 6 hours in cultures exposed to falling gentamicin concentrations, and after 8 hours in cultures kept at the MIC. Thus, a postulated superiority of gentamicin infusions over intermittent gentamicin therapy could not be demonstrated in vitro. Intervals between bolus injections of gentamicin should probably not be longer than 6 hours.
D003114:Colony-Forming Units Assay; D003692:Delayed-Action Preparations; D005839:Gentamicins / Q000008:administration & dosage* / Q000494:pharmacology; D006207:Half-Life; D066298:In Vitro Techniques; D007263:Infusions, Parenteral; D011550:Pseudomonas aeruginosa / Q000187:drug effects*
D003692:Delayed-Action Preparations; D005839:Gentamicins
In-vitro-Studie zur Frage der optimalen Gentamicintherapie bei leukopenischen Patienten: Kuzinfusion oder Dauerinfusion?