Organ cultured donor material for penetrating corneal grafts. A preliminary report.
Fresh and undamaged cornea had unchanged endothelial cell density for four weeks in culture, while eyes removed at autopsy suffered a cell loss. Acceptable cell density was found in eyes removed within eight hours, and this was confirmed by the results of 55 penetrating grafts using cultured autopsy material. Storage in culture for more than one week seemed to influence the results unfavourably. Immune reactions were not seen.
D002470:Cell Survival; D003315:Cornea / Q000473:pathology; D016039:Corneal Transplantation; D003470:Culture Media; D004727:Endothelium / Q000166:cytology; D006801:Humans; D009924:Organ Culture Techniques / Q000379:methods*; D009926:Organ Preservation; D013194:Staining and Labeling; D013997:Time Factors; D014019:Tissue Donors; D014022:Tissue Survival; D014184:Transplantation, Homologous
D003470:Culture Media