Immunohistochemical localization of motilin in endocrine non-enterochromaffin cells of the small intestine of humans and monkey.
Motilin-immunoreactive cells in the human and monkey duodenum and upper jejunum were investigated by immunofluorescence (IF) and peroxidase-antiperoxidase (PAP) techniques using antibodies against synthetic 13-norleucine motilin and synthetic porcine motilin. Contrary to previous reports, we have demonstrated that motilin-immunoreactive cells are a distinct cell population that does not correspond to 5-hydroxytryptamine-containing enterochromaffin (EC-) cells. EC-cells, indentified by formaldehyde-induced fluorescence (FIF) or by argentaffinity (AA), do not react with either antisera. EC-cells of the monkey, utilizing one method (PAP), reacted to one antiserum very weakly. This reaction was also suppressed by absorption with 13-norleucine motilin. It is suggested that the EC-cells of the monkey contain either a very small amount of motilin or another peptide that exhibits a part of the amino acid sequence of motilin. The discrepancy between these results and those of other authors can be explained by the different reactivity of the antisera used.
D000818:Animals; D002838:Chromaffin System / Q000378:metabolism*; D004386:Duodenum / Q000378:metabolism; D004759:Enterochromaffin Cells / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D005768:Gastrointestinal Hormones / Q000378:metabolism*; D000882:Haplorhini; D006801:Humans; D007082:Ileum / Q000378:metabolism; D007124:Immunoenzyme Techniques; D007421:Intestine, Small / Q000166:cytology / Q000378:metabolism*; D007583:Jejunum / Q000378:metabolism; D009037:Motilin / Q000378:metabolism*; D012701:Serotonin / Q000378:metabolism
D005768:Gastrointestinal Hormones; D012701:Serotonin; D009037:Motilin