title: Mutations affecting immunoglobulin light chain secretion by myeloma cells. I. Functional analysis by cell fusion.
abstract: Two clones of MOPC 315 cells have been selected which synthesize but do not secrete Ig lambda light chains. These clones were analyzed by fusion with a cell line synthesizing and secreting kappa chains. Conditions were established for recovery at high frequency (approximately 10(-3)) of spontaneously fused, viable hybrid cells. The resulting hybrid cell lines synthesized both kappa and lambda chains but secreted only kappa chains. Hybrid cells produced by fusion of a lambda-secreting clone of MOPC 315 with the kappa-secreting cell line were also isolated and shown to synthesize and secrete both kappa and lambda chains. These results suggest that the nonsecretion of lambda chains was not due to a defect the secretion mechanism of the variant cells. A more likely alternative is that the lambda chains in the variant cell lines were structurally altered to a form which could not be secreted.
mesh_terms: D000818:Animals; D002459:Cell Fusion; D002999:Clone Cells / Q000276:immunology; D006822:Hybrid Cells; D007106:Immune Sera / Q000494:pharmacology; D007147:Immunoglobulin Light Chains / Q000096:biosynthesis* / Q000235:genetics; D007146:Immunoglobulin lambda-Chains / Q000276:immunology; D009101:Multiple Myeloma / Q000378:metabolism*; D009154:Mutation; D010641:Phenotype; D011817:Rabbits
chemical_list: D007106:Immune Sera; D007147:Immunoglobulin Light Chains; D007146:Immunoglobulin lambda-Chains