[Isotachophoretic isolation of IgD and IgE and production of monospecific antisera against them].
The method of preparative isotachophoresis in acrylamide gel ensuring a high yield of IgD and IgE with insignificant admixtures of IgG, etc. was used for the isolation of IgD and IgE from the blood sera of myeloma patients. As a result of immunization with these antigens, monospecific IgD and IgE antisera were obtained. These antisera, alongside with specific antibodies, contained antibodies to admixtures; the latter were eliminated by the method of immune absorbtion carried out with the use of a sorbent based on Sepharose activated with bromo-cyanogen and conjugated with normal human blood serum. Ig D antisera were also shown to contain antibodies to idiotypical IgD determinants located in the Fab fragment of this immunoglobulin.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000096:biosynthesis* / Q000302:isolation & purification; D002682:Chinchilla / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006801:Humans; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007072:Immunoglobulin D / Q000276:immunology / Q000302:isolation & purification*; D007073:Immunoglobulin E / Q000276:immunology / Q000302:isolation & purification*; D007140:Immunoglobulin Fab Fragments / Q000276:immunology; D007163:Immunosorbent Techniques; D009101:Multiple Myeloma / Q000378:metabolism
D000888:Antibodies, Anti-Idiotypic; D007072:Immunoglobulin D; D007140:Immunoglobulin Fab Fragments; D007073:Immunoglobulin E
Vydelenie metodom izotakhoforeza IgD i IgE i poluchenie k nim monospetsificheskikh antisyvorotok.