A rapid method for the purification of antibody--enzyme conjugates.
A method is described for a rapid and efficient separation of enzyme-labelled antibodies from the free enzyme following the coupling reaction. A single passage of the reaction mixture on a protein A--Sepharose CL-4B column gave a sharp separation of the free enzyme from the conjugate.
D000906:Antibodies / Q000302:isolation & purification*; D005779:Immunodiffusion; D007124:Immunoenzyme Techniques; D013205:Staphylococcal Protein A
D000906:Antibodies; D013205:Staphylococcal Protein A