Studies of long-range density effects on the proliferation of 3T3 and RLCW cells in recirculated medium.
Swiss mouse 3T3 cells and rat liver-derived RLCW cells were grown in monolayers and perfused with culture medium. A flow-rate dependent increase in the growth rate was observed both by 3H-thymidine uptake and by a rise in cell numbers. The characteristics of the response were dependent on the recirculating volume and on whether serum was present in the culture medium. In RLC cultures perfused with serum-supplemented medium the growth promoting effect decreased with increasing density of the cells. In the absence of serum, recirculation of NCTC medium had no effect on RLCs but increased growth was observed in recirculated MEM. In 3T3 cultures, a linear response was observed over a limited density range in the presence of 10% serum-supplemented medium indicating that substances present in the serum substantially modify the behaviour of the monolayer to perfusion. In serum-free medium the effect of perfusion on 3T3 cultures was confined to a small density range and was consistent with the more rapid removal of a diffusible inhibitor from the pericellular environment by recirculating the medium. Treatment of the perfusing medium with immobilised proteinases (trypsin, chymotrypsin, protease) did not alter the response except in the presence of putrescine.
D002452:Cell Count; D002478:Cells, Cultured / Q000166:cytology*; D002918:Chymotrypsin; D003470:Culture Media; D003584:Cytological Techniques; D004800:Enzymes, Immobilized; D010447:Peptide Hydrolases; D013305:Streptomyces griseus / Q000201:enzymology; D014357:Trypsin
D003470:Culture Media; D004800:Enzymes, Immobilized; D010447:Peptide Hydrolases; D002918:Chymotrypsin; D014357:Trypsin