[Synthesis of virus-specific products following introduction of tobacco mosaic virus RNA pereparations and the native virus into acetabularia].
The possibility to synthesize the viral-specific products after microinjection of Tobacco mosaic virus (TMV) preparations and the TMV RNA into the single-celled seaweed Acetabularia was studied. The accumulation of the newly synthesized protein and double-stranded RNA 24 hours after injection of TMV RNA and native virus preparations was demonstrated by immunological and immunofluorescent methods. The virus titer sharply dropped 3--4 hours after introduction into Acetabularia and in 48 hours it reached a maximum level. The presented data showed the possibility of TMW RNA replication and translation involving formation of viral-specific proteins and the production of a virus of full value in the Acetabularia cell.
D000076:Acetabularia / Q000378:metabolism*; D001345:Autoradiography; D000460:Chlorophyta / Q000378:metabolism*; D002850:Chromatography, Gel; D005455:Fluorescent Antibody Technique; D005779:Immunodiffusion; D012367:RNA, Viral / Q000096:biosynthesis / Q000378:metabolism*; D014027:Tobacco Mosaic Virus; D014764:Viral Proteins / Q000096:biosynthesis; D014779:Virus Replication
D012367:RNA, Viral; D014764:Viral Proteins
Izuchenie sinteza virusspetsificheskikh produktov posle vvedeniia v atsetabuliariiu preparatov RNK virusa tabachnoĭ mozaiki i nativnogo virusa.