title: Chelating agents protect hydrogenase against oxygen inactivation.
abstract: The effect of chelation on rate or air inactivation of hydrogenase from Clostridium pasteurianum has been investigated. All chelating agents used, whether water-soluble or water-insoluble, afforded protection against oxygen inactivation. EDTA appeared to be the most effective. Thus, in the absence of EDTA, hydrogenase in aqueous solution was nearly totally inactivated after 1 hour incubation in air, whereas 0.5 M EDTA (which did not affect significantly catalytic activity) allowed 41% retention of the initial activity even after 3 days incubation.
mesh_terms: D002614:Chelating Agents / Q000494:pharmacology*; D003013:Clostridium / Q000201:enzymology*; D004492:Edetic Acid / Q000494:pharmacology; D005288:Ferredoxins; D006859:Hydrogen; D007700:Kinetics; D010088:Oxidoreductases / Q000378:metabolism*; D010100:Oxygen; D010618:Phenanthrolines / Q000494:pharmacology
chemical_list: D002614:Chelating Agents; D005288:Ferredoxins; D010618:Phenanthrolines; D006859:Hydrogen; D004492:Edetic Acid; D010088:Oxidoreductases; D010100:Oxygen