Demonstration of two disease specific antigens in circulating immune complexes.
A method is described to assess antigenic cross-reactivity between soluble immune complexes precipitated from sera with polyethylene glycol. The precipitated complex from one serum was dissociated in acid and used to coat a plastic cup. Radioiodinated complexes from another serum were dissociated in the cup, neutralized and allowed to reassociate overnight. The binding of the labelled complex was used to measure the cross-reactivity between the complexes. Using this technique, complexes from a group of patients with haematuria and hypertension have been found to share an antigen, and a different antigen was found in patients with bullous pemphigoid. The participation of rheumatoid factors in the cross-reactions is unlikely, and no cross-reactivity of either group was found with sera from patients with rheumatoid arthritis.
D000328:Adult; D000368:Aged; D000936:Antigen-Antibody Complex; D001172:Arthritis, Rheumatoid / Q000276:immunology; D003429:Cross Reactions; D000939:Epitopes; D006801:Humans; D006973:Hypertension / Q000276:immunology; D008722:Methods; D008875:Middle Aged; D011092:Polyethylene Glycols; D012710:Serum Albumin, Bovine / Q000276:immunology; D012872:Skin Diseases, Vesiculobullous / Q000276:immunology
D000936:Antigen-Antibody Complex; D000939:Epitopes; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols