Beta-lactamases: determination of their isoelectric points.
Important discrepancies in isoelectric points (pI) of beta-lactamases were observed depending on the experimental procedure used for their determination: isoelectric focusing (IEF) in sucrose density gradients or analytical IEF in thin-layer polyacrylamide gels (PA). The variations, negligable in the case of TEM-like beta-lactamases, appeared to be important in the case of cephalosporinases and are related to an artifact which appears in PA-IEF. This has been clearly shown with beta-lactamase preparations from the following bacterial strains: Pseudomonas aeruginosa NCTC 8203 (pI = 8.7), P. aeruginosa NCTC 10701 (pI = 9.4), and Proteus morganii NCTC 235 (pI = 8.3). The data previously obtained by PA-IEF were much lower.
D000581:Amidohydrolases / Q000032:analysis*; D002510:Cephalosporinase / Q000032:analysis*; D004926:Escherichia coli / Q000201:enzymology; D007525:Isoelectric Focusing / Q000379:methods; D007526:Isoelectric Point; D008970:Molecular Weight; D011550:Pseudomonas aeruginosa / Q000201:enzymology
D000581:Amidohydrolases; D002510:Cephalosporinase