[Preparative electrophoresis of peptidic glycosaminoglycans. Application to the fractionation of peptidic glycosaminoglycans of arterial wall (author's transl)].
A new procedure for the fractionation of glycosaminoglycans by electrophoresis on Pevikon has been described. Mixtures of glycosaminoglycans were fractionated by preparative electrophoresis on Pevikon in pyridine formate or glycine-HCl buffers. By this procedure, 200 mg of a mixture of hyaluronic acid, heparan sulphate, dermatan sulphate and chondroitin sulphate isolated from arterial wall could be successfully separated without loss of material. The purified fractions were analysed by enzymatic and chemical procedures. The molar ratios of uronic acid to hexosamine and of sulphate to hexosamine and the amino acid content of each glycosaminoglycan have been determined. The peptidic content (less than 1%) is represented by five amino acids, viz. serine, glycine, alanine, aspartic acid, and glutamic acid.
D000596:Amino Acids / Q000032:analysis; D001158:Arteries / Q000737:chemistry*; D002021:Buffers; D003871:Dermatan Sulfate / Q000032:analysis; D004586:Electrophoresis; D006025:Glycosaminoglycans / Q000032:analysis*; D006497:Heparan Sulfate / Q000032:analysis; D006595:Hexosamines / Q000032:analysis; D006820:Hyaluronic Acid / Q000032:analysis; D008722:Methods; D010455:Peptides / Q000032:analysis*
D000596:Amino Acids; D002021:Buffers; D003871:Dermatan Sulfate; D006025:Glycosaminoglycans; D006497:Heparan Sulfate; D006595:Hexosamines; D006820:Hyaluronic Acid; D010455:Peptides
Electrophorèse préparative des glycosaminoglycanes-peptides. Application au fractionnement des glycosaminoglycanes-peptides de la paroi artérielle