The effects of temperature and inhibitors on HCO3-stimulated swelling and ion uptake of monkey cerebral cortex.
In the presence of high concentrations of K+, additions of HCO3- as low as 0.35 mM caused a 23% increase in swelling, and concomitant increases in the chloride content of incubating monkey cerebrocortical slices. The uptake of chloride was accompanied by increased uptake of sodium and was highly temperature dependent, showing a marked activation at approximately 30 degrees C. A similar temperature activation was also found for a Mg2+-dependent, HCO3-stimulated ATPase activity in monkey cerebral cortex, consistent with a possible role for this enzyme in the K+ and HCO3-dependent swelling process and its associated ion movements. K+-dependent, HCO3-stimulated cerebrocortical tissue swelling with uptake of Na+ and Cl- was inhibited by acetazolamide indicating that carbonic anhydrase was also involved. The addition of ouabain also inhibited swelling and K+ and Cl- uptake at low concentrations, but led to increased swelling at higher concentrations ( greater than 10 mum). A similar biphasic effect on swelling was also seen following addition of ethacrynic acid.
D000086:Acetazolamide / Q000494:pharmacology; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D001639:Bicarbonates / Q000494:pharmacology*; D002256:Carbonic Anhydrases / Q000378:metabolism; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D002712:Chlorides / Q000378:metabolism; D004976:Ethacrynic Acid / Q000494:pharmacology; D000882:Haplorhini; D006801:Humans; D008253:Macaca mulatta; D008274:Magnesium; D009457:Neuroglia / Q000201:enzymology / Q000378:metabolism; D010042:Ouabain / Q000494:pharmacology; D011188:Potassium; D012964:Sodium / Q000378:metabolism; D013696:Temperature
D001639:Bicarbonates; D002712:Chlorides; D010042:Ouabain; D012964:Sodium; D000251:Adenosine Triphosphatases; D002256:Carbonic Anhydrases; D008274:Magnesium; D004976:Ethacrynic Acid; D000086:Acetazolamide; D011188:Potassium