title: Mithramycin fluorescence for quantitative determination of deoxyribonucleic acid in single cells.
abstract: The use of the antibiotic drug mithramycin for cytoflurometric assessment of deoxyribonucleic acid in single cells has been studied in smears of a standard cell population of rat thymocytes. The optimal staining conditions have been determined including the influence of fixation (freeze-drying, formalin and ethanol). The drug equilibration time has been estimated in relation to the concentration of the mithramycin and to Mg++ was found to enhance the fluorescence intensity produced by the mithramycin-deoxyribonucleic acid interaction. The stability and the reproducibility of the fluorescence reaction are discussed.
mesh_terms: D000818:Animals; D001665:Binding Sites; D004247:DNA / Q000032:analysis*; D005260:Female; D008274:Magnesium / Q000494:pharmacology; D008856:Microscopy, Fluorescence; D008926:Plicamycin; D051381:Rats; D013950:Thymus Gland / Q000032:analysis* / Q000648:ultrastructure
chemical_list: D004247:DNA; D008274:Magnesium; D008926:Plicamycin