Inhibition of key glycolytic enzymes from rabbit kidney medulla by free fatty acids in vitro.
In vitro incubation of key glycolytic enzymes in supernatant fluids from rabbit kidney medulla with increasing concentrations of sodium laurate resulted in progressive inhibition of hexokinase, phosphofructokinase and pyruvate kinase. A corresponding reduction in the production of lactate from glucose was also observed. The possible effects of these enzyme inhibitions on the naturesis observed during fasting are discussed.
D000818:Animals; D006019:Glycolysis / Q000187:drug effects*; D006593:Hexokinase / Q000378:metabolism; D007668:Kidney / Q000201:enzymology*; D007679:Kidney Medulla / Q000187:drug effects / Q000201:enzymology*; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007850:Lauric Acids / Q000494:pharmacology*; D010732:Phosphofructokinase-1 / Q000378:metabolism; D011770:Pyruvate Kinase / Q000378:metabolism; D011817:Rabbits
D007850:Lauric Acids; D007770:L-Lactate Dehydrogenase; D006593:Hexokinase; D010732:Phosphofructokinase-1; D011770:Pyruvate Kinase