Sponge matrix allografts. A model for analysis of killer cells infiltrating mouse allografts.
A method for isolation of allograft-infiltrating cells in a functionally viable state is described in this article. The method is based on the use of a spongious tissue into which cells of strain A (e.g., fibroblasts or tumor cells) are grown. The resulting graft is then transplanted to a strain B animal, and the infiltrating cells are released from it by gentle compression. The graft-infiltrating cells are completely recovered, and they may be processed for further experimentation and analysis by employing exclusively physical methods of cell preparation. As an example of future applications, some preliminary results on density- and charge fractionation of the graft-infiltrating cells are also reported.
D000818:Animals; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D003601:Cytotoxicity Tests, Immunologic; D004586:Electrophoresis; D006084:Graft Rejection; D051379:Mice; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D008954:Models, Biological; D010529:Peritoneal Cavity / Q000166:cytology; D011093:Polyethylene Terephthalates; D011138:Polytetrafluoroethylene; D013523:Surgical Equipment; D014184:Transplantation, Homologous / Q000379:methods*
D011093:Polyethylene Terephthalates; D011138:Polytetrafluoroethylene