[Molecular forms of human growth hormone (HGH) secreted by cultured fetal pituitary cells].
Cultures of foetal human pituitary cells excrete and synthetize different molecular forms of proteins with HGH immunological activity. These cells incorporate tritiated-leucine in these proteins. Gel chromatography on sephadex using different length of column allow us to separate: One form excluded in front of the dead volume and which has a high molecular weight. This form is not dissociated by treatment with urea 8 M, guanidine 6 M and dithiothreitol. Nor this form is modified by treatment by ribonuclease. One form excluded within the dead volume and which is probably a dimere. This form is no more modified by the different treatments. One form which is excluded like a monomere--it is the more important form--. This form is dissociated in fragments of lower molecular weight by urea 8 M. This dissociation is partially reversible by dialysis.
D002478:Cells, Cultured; D002850:Chromatography, Gel; D013006:Growth Hormone / Q000032:analysis; D006801:Humans; D008968:Molecular Conformation; D008970:Molecular Weight; D010902:Pituitary Gland / Q000378:metabolism*; D010903:Pituitary Gland, Anterior / Q000196:embryology / Q000378:metabolism*
D013006:Growth Hormone
Formes moléculaires de l'hormone de croissance humaine (HGH) sécrétées par des cellules hypophysaires foetales en culture