Amino-acid sequence of the cyanogen-bromide fragment from human plasminogen that forms the linkage between the plasmin chains.
The complete amino acid sequence of a cyanogen bromide fragment (122 residues) obtained from plasminogen is described. This fragment forms the overlap between heavy (A) and light (B) chains of human plasmin. The particular arginyl-valyl bond cleaved in the second step of the activation process is shown to be Arg98-Val99 in this fragment. This site is not very similar to the one in the NH2-terminal part of the molecule (Arg68-Met69). Remarkable homologies with the 'triple loops' ('kringle structures') found in the non-thrombin part of prothrombin are demonstrated. Homologies occurred during evolution of this chain.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D003488:Cyanogen Bromide; D005341:Fibrinolysin / Q000032:analysis; D006801:Humans; D008722:Methods; D010446:Peptide Fragments / Q000032:analysis; D010958:Plasminogen / Q000032:analysis*
D000596:Amino Acids; D010446:Peptide Fragments; D010958:Plasminogen; D005341:Fibrinolysin; D003488:Cyanogen Bromide