[Mechanism of oxidation reaction of NADH models and phynylglyoxal with hydrogen peroxide. Hypothesis on separate transport of hydrogen and electron atom in certain enzymatic reactions with the participation of NADH and NADPH].
Kinetics of co-oxidation of 1-benzen-3-carbamido-1,4-dihydropyridine (BDN) and phenylglyoxal (PG) with hydrogen peroxide is studied. Dimeric product (di-e11-benzen-5-carbamido-1,2-dihydropyridyl-2]) is found to be formed at pH 9, and quaternal pyridinium salt (BNA)--at pH 7. Molecular oxigen is determined to participate in the reaction at pH 7. Copper (II) ions catalyze this process. Significant catalytic effect of p-dinitrobenzen (p-DNB) is found. The reaction mechanism is postulated to form hydroperoxide from PG and hydrogen peroxide which are capable to split the hydrogen attom from dihydropyridine, molecular oxigen or p-DNB being an acceptor of the electrone. Hypothesis on separate transfer of hydrogen atom and electrone in biological systems are proposed.
D000447:Aldehydes / Q000031:analogs & derivatives*; D002845:Chromatography / Q000379:methods; D004136:Dinitrobenzenes / Q000494:pharmacology; D006037:Glyoxal / Q000031:analogs & derivatives* / Q000378:metabolism; D006859:Hydrogen / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism*; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism* / Q000494:pharmacology; D009536:Niacinamide / Q000031:analogs & derivatives* / Q000378:metabolism; D010084:Oxidation-Reduction
D000447:Aldehydes; D004136:Dinitrobenzenes; D009536:Niacinamide; D006037:Glyoxal; D006859:Hydrogen; D006861:Hydrogen Peroxide; D009247:NADH, NADPH Oxidoreductases
Mekhanizm reaktsiĭ okisleniia modeleĭ NADH i fenilglioksalia perekis'iu vodoroda. Gipoteza o razdel'nom perenose atoma vodoroda i electrona v nekotorykh fermentativnykh reaktsiiakh s uchastiem NADH NADPH