[Isolation of a new keto-acid, produced from epsilon-N-trimethyllysine by Neurospora crassa].
A new epsilon-N-trimethyllysine metabolite has been isolated from the mycelium of Neurospora crassa. The labelled compound produced from incubations in vivo and in vitro from epsilon-N-trimethyl ([14-C]H3)L-lysine has been identified as 2-keto-epsilon-N-trimethyl-hexanoic acid by reducing its 2,4-dinitrophenyl hydrazone back to epsilon-N-trimethyllysine by hydrogenolysis in a Parr bomb. Analyses on TLC and in four different ion exchange chromatographic systems show the appearance of a ninhydrin positive product having the same Rf and the same retention time as epsilon-N-trimethyllysine; it contains more than 85% of the radioactivity of the 2,4-dinitrophenylhydrazone of the keto acid.
D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D007651:Keto Acids / Q000096:biosynthesis*; D008239:Lysine / Q000032:analysis* / Q000378:metabolism; D009491:Neurospora / Q000378:metabolism*; D009492:Neurospora crassa / Q000378:metabolism*
D007651:Keto Acids; D008239:Lysine
Isolement d'un nouveau ceto-acide, produit a partir de epsilon-N-trimethyllsine par Neurospora crassa