A lymphocyte chalone assay using lymphocyte colony growth in agar capillaries.
Using a recently developed method of culturing T-lymphocyte colonies in agar-containing capillary tubes, the capacity of three different lymphoid extracts with lymphocyte chalone (LC) activity to inhibit colony growth was demonstrated. Sephadex fractions from a calf spleen extract were tested on the colony growth of granulocytic cells and PHA-stimulated T-lymphocytes as well as on the in vitro uptake of 3H-thymidine by bone marrow and ConA- and LPS-stimulated mouse spleen cells. The data strongly suggest that it is only the combination of several different assay systems applied to the same fractions that permits a clear-cut determination of a specific lymphocyte proliferation inhibitor like LC.
D000362:Agar; D000818:Animals; D001681:Biological Assay / Q000379:methods*; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D003208:Concanavalin A / Q000494:pharmacology; D046508:Culture Techniques; D006098:Granulocytes / Q000187:drug effects; D006131:Growth Inhibitors / Q000494:pharmacology*; D037102:Lectins / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008198:Lymph Nodes; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D013154:Spleen; D013601:T-Lymphocytes / Q000187:drug effects*; D013936:Thymidine / Q000378:metabolism; D014020:Tissue Extracts / Q000494:pharmacology*
D006131:Growth Inhibitors; D037102:Lectins; D008070:Lipopolysaccharides; D014020:Tissue Extracts; D003208:Concanavalin A; D000362:Agar; D013936:Thymidine