Induction by RNA of inositol independence in Neurospora crassa.
The effect of purified wild-type RNA (allo-RNA) on genetic reversion of inositol-requiring mutant 89601 of Neurospora crassa is described. The mutant (inos minus) strain, on treatment with the wild-type RNA preparation, was found to revert to wild type (inos+) in significant numbers. RNA from the mutant (iso-RNA) and allo-RNA digested by RNase were ineffective in causing genetic reversion at the inositol locus. The allo-RNA-induced revertants were stable and showed a Mendelian transmission of the inos+ character.
D007294:Inositol / Q000378:metabolism*; D009154:Mutation; D009491:Neurospora / Q000378:metabolism*; D009492:Neurospora crassa / Q000378:metabolism*; D010641:Phenotype; D012313:RNA / Q000378:metabolism*; D012260:Ribonucleases / Q000378:metabolism; D014170:Transformation, Genetic
D007294:Inositol; D012313:RNA; D012260:Ribonucleases