Localization of the membrane perturber adamantylsulfate in sarcoplasmic reticular vesicles.
The properties of a new membrane perturbing agent, adamantyl sulfate (A-1-S) are described. Lobster sarcoplasmic reticular vesicles (SRV) were used as a test membrane system and the activity of the Ca2+--Mg2+-dependent ATPase in conjunction with spin label data was used to determine the location of A-1-S in the membrane. The results suggest that A-1-S perturbs only the polar region of the SRV and that this causes loss of ATPase function. Thus, ATPase activity requires integrity of the membrane polar region.
D000218:Adamantane / Q000494:pharmacology*; D000251:Adenosine Triphosphatases / Q000378:metabolism*; D001952:Bridged-Ring Compounds / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D003902:Detergents / Q000494:pharmacology; D010743:Phospholipids; D012519:Sarcoplasmic Reticulum / Q000187:drug effects* / Q000201:enzymology; D013113:Spin Labels
D001952:Bridged-Ring Compounds; D003902:Detergents; D010743:Phospholipids; D013113:Spin Labels; D000251:Adenosine Triphosphatases; D000218:Adamantane; D002118:Calcium