[Method of rapid separation of free, sulfoconjugated and glucuronoconjugated fractions of plasma steroids].
A method for the separation of serum conjugated steroids, sulfates and glucuronides, is described. It is based on the fact that methylene blue forms complexes with ester-sulfates which can be quantitatively and specifically extracted by chloroform from aqueous phase. The quantitative determination of the conjugated fractions thus obtained can be done after solvolysis followed by hydrolysis by beta-glucuronidase from Helix pomatia.
D005591:Chemical Fractionation / Q000379:methods; D003687:Dehydroepiandrosterone / Q000097:blood; D004958:Estradiol / Q000097:blood; D004970:Estrone / Q000097:blood; D005260:Female; D005965:Glucuronates / Q000097:blood / Q000302:isolation & purification; D005966:Glucuronidase; D006801:Humans; D008297:Male; D008751:Methylene Blue; D013256:Steroids / Q000097:blood* / Q000302:isolation & purification; D013431:Sulfates / Q000097:blood / Q000302:isolation & purification; D014316:Tritium
D005965:Glucuronates; D013256:Steroids; D013431:Sulfates; D014316:Tritium; D004970:Estrone; D003687:Dehydroepiandrosterone; D004958:Estradiol; D005966:Glucuronidase; D008751:Methylene Blue
Méthode de séparation rapide des fractions libre, sulfoconjuguée et glucuroconjuguée des stéroïdes plasmatiques