[Perfection of an experimental schedule for the quantitative evaluation of trans-abscissic acid by biological tests].
Using gas-liquid chromatography, we have studied the kinetics of the isomerization from trans-ABA to cis-ABA under the effect of short ultraviolet-light (254 nm). At first, for a given volume, the conditions required to reach 50% isomerization were determined. Then the method was adapted to the use of extracts. It is thus possible to estimate the amount of trans-ABA in extracts with a suitable biological test.
D000040:Abscisic Acid / Q000032:analysis*; D001681:Biological Assay; D002849:Chromatography, Gas; D002853:Chromatography, Liquid; D003509:Cyclohexanecarboxylic Acids / Q000032:analysis*; D007536:Isomerism; D007700:Kinetics; D000432:Methanol; D010936:Plant Extracts / Q000032:analysis; D012997:Solvents; D014466:Ultraviolet Rays
D003509:Cyclohexanecarboxylic Acids; D010936:Plant Extracts; D012997:Solvents; D000040:Abscisic Acid; D000432:Methanol
Mise au point d'un protocole expérimental permettant d'évaluer quantitativement l'acide trans-abscissique à l'aide de tests biologiques