Effect of cholesterol oxidation on (Na+, K+) ATPase activity of erythrocyte membranes.
By incubation of human erythrocyte ghosts with cholesterol oxidase (EC 1.1.3.6) part of the cholesterol of the membrane is replaced by 4-cholesten-3-one. This alteration in the sterol composition is accompanied by an inhibition of the (Na+, K+) ATPase of the erythrocyte membrane.
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology; D002784:Cholesterol / Q000378:metabolism*; D004912:Erythrocytes / Q000187:drug effects / Q000201:enzymology*; D006801:Humans; D006913:Hydroxysteroid Dehydrogenases / Q000494:pharmacology; D008274:Magnesium / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D012964:Sodium / Q000494:pharmacology
D002784:Cholesterol; D012964:Sodium; D006913:Hydroxysteroid Dehydrogenases; D000251:Adenosine Triphosphatases; D008274:Magnesium; D011188:Potassium