[Characterisation of RNA from synchronously growing yeast cells (Saccharomyces cerevisiae) as separated by columns of methylated albumin on Kieselgur (MAK) (author's transl)].
Total RNA, extracted from synchronously growing yeast cells by the method of Georgiev-Mantieva, was separated by columns of methylated albumin on Kieselgur (MAK). By comparison with definited RNA's in discelectrophorese and after pulse labelling with [3H]methionine the following fractions are identified: t-RNA, 5.8S RNA, m-RNA, 18S and 25S R-RNA and their precursors 20S, 27S and 35S. High molecular fractions of RNA between 40S and 70S were also found.
D002845:Chromatography; D004589:Electrophoresis, Disc; D008715:Methionine; D012313:RNA / Q000032:analysis*; D012333:RNA, Messenger / Q000032:analysis; D012335:RNA, Ribosomal / Q000032:analysis; D012343:RNA, Transfer / Q000032:analysis; D012441:Saccharomyces cerevisiae / Q000032:analysis*
D012333:RNA, Messenger; D012335:RNA, Ribosomal; D012313:RNA; D012343:RNA, Transfer; D008715:Methionine
Charakterisierung von RNA aus synchron wachsender Bäcker-Hefe (Saccharomyces cerevisiae) bei Trennung mit der Methylalbumin-Kieselgur (MAK)-Säule