title: [Isolation of active preparation of Na+, K+-ATPase from cattle brain and study of the role of carboxyl, sulfhydryl and hydroxyl groups].
abstract: A method is presented for obtaining the preparation of N+, K+-ATPase from the cattle brain. The specific activity of the preparation is 5 units (mu mole Pi per 1 min) per 1 mg of protein. A water-soluble derivate of carbodiimide is shown to inhibit reversibly both Na+, K+-ATPase and K+-phosphatase. ATP, Na+ and K+ manifest a protective effect against inhibition, and Na+ and K+ revealed a competition with the inhibitor for the enzyme. p-Chloromercuribenzoate inhibits irreversibly Na+, K+-ATPase and K+-phosphatase activities. The substrates ATP and p-nitrophenylphosphate protected these activities against inhibition. The phosphororganic compound O-n-butyl-S-(beta-ethyl-mercaptoethyl)-methyl thiophosphate has no significant effect on the Na+, K+-ATPase and K+-phosphatase activities.
mesh_terms: D000251:Adenosine Triphosphatases / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000201:enzymology*; D002234:Carbodiimides / Q000494:pharmacology; D002417:Cattle; D002729:Chloromercuribenzoates / Q000494:pharmacology; D004789:Enzyme Activation; D009596:Nitrophenols / Q000494:pharmacology; D009943:Organophosphorus Compounds / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D011485:Protein Binding; D012964:Sodium / Q000494:pharmacology
chemical_list: D002234:Carbodiimides; D002729:Chloromercuribenzoates; D009596:Nitrophenols; D009943:Organophosphorus Compounds; D012964:Sodium; D000251:Adenosine Triphosphatases; D011188:Potassium