K+--induced changes of oxygen uptake by neuronal enriched and glial enriched fractions from mouse brain cortex.
Neuronal and glial enriched fractions were incubated in a medium with 10mM pyruvate, 5mM fumarate and 0.9mM 5'-AMP and the effect of increased external K+ concentrations was studied upon oxygen uptake. A concentration of 65 mM K+ had a different effect on the oxygen consumption of glial and neuronal perikarya. The rate of oxygen uptake by glia was stimulated by 52.81% whilst an insignificant decrease of 15.79% occurred in the neurones. The highest rate of oxygen uptake by incubated cells was estimated in the presence of the substrate system containing pyruvate, fumarate and 5'-AMP. The significance of components in the substrate system for a high rate of oxygen uptake by cells was also tested with 6.2 mM K+ and 65 mM K+.
D000818:Animals; D002458:Cell Fractionation; D002540:Cerebral Cortex / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D005110:Extracellular Space; D051379:Mice; D008805:Mice, Inbred A; D009457:Neuroglia / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D010101:Oxygen Consumption / Q000187:drug effects*; D011188:Potassium / Q000494:pharmacology*
D011188:Potassium