A simple and rapid method for the preparation of adenosine triphosphatase from submitochondrial particles.
An almost pure form of the bovine heart mitochondrial adenosine triphosphatase (ATPase) is released from the membrane by shaking submitochondrial particles with chloroform. Analyses on polyacrylamide gels and by electron microscopy, and also sensitivity to inhibitors, show that the chloroform-released enzyme is similar to other ATPase preparations from bovine heart mitochondria.
D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors / Q000302:isolation & purification*; D000818:Animals; D001313:Aurovertins / Q000494:pharmacology; D002417:Cattle; D002725:Chloroform; D004024:Dicyclohexylcarbodiimide / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D066298:In Vitro Techniques; D008722:Methods; D008854:Microscopy, Electron; D008931:Mitochondria, Muscle / Q000201:enzymology*; D009206:Myocardium / Q000201:enzymology*; D013347:Subcellular Fractions / Q000032:analysis; D014461:Ultracentrifugation
D001313:Aurovertins; D004024:Dicyclohexylcarbodiimide; D002725:Chloroform; D000251:Adenosine Triphosphatases