Analysis of urinary mucopolysaccharides using small ion exchange columns.
Methods are presented for the small scale column chromatography of urinary mucopolysaccharides. After isolation by cety pyridinium chloride mucopolysaccharides are applied to a small column of ion exchange resin and eluted by 2 M-sodium chloride. Colorimetric assays on this elutate allow clinically significant disease states to be detected.
D011232:Chemical Precipitation; D002852:Chromatography, Ion Exchange; D005688:Galactosamine / Q000032:analysis; D005944:Glucosamine / Q000032:analysis; D006025:Glycosaminoglycans / Q000652:urine*; D006801:Humans; D006821:Hyaluronoglucosaminidase; D008722:Methods; D009083:Mucopolysaccharidoses / Q000652:urine; D012965:Sodium Chloride; D014574:Uronic Acids / Q000032:analysis
D006025:Glycosaminoglycans; D014574:Uronic Acids; D012965:Sodium Chloride; D005688:Galactosamine; D006821:Hyaluronoglucosaminidase; D005944:Glucosamine