Amino acid replacement in the protein S5 from a spectinomycin resistant mutant of Bacillus subtilis.
Ribosomal protein S5 was isolated from wild type Bacillus subtilis ATCC 6633 and from a spectinomycin resistant mutant (BSPC 111) derived from spectinomycin sensitive to resistance is accomtrypsin and all the tryptic peptides were isolated by column- and paper-chromatography. By comparative amino acid analyses of the peptides, it was demonstrated that the S5 from the mutant differs from the wild type S5 by a replacement of one amino acid, namely lysine by isoleucine in the peptide T9. The results are compared with E. coli spectinomycin resistant mutants.
D000595:Amino Acid Sequence; D001412:Bacillus subtilis / Q000187:drug effects / Q000378:metabolism*; D001426:Bacterial Proteins / Q000032:analysis*; D002854:Chromatography, Paper; D004352:Drug Resistance, Microbial; D009154:Mutation; D012269:Ribosomal Proteins / Q000032:analysis*; D000198:Spectinomycin / Q000494:pharmacology; D014357:Trypsin / Q000494:pharmacology
D001426:Bacterial Proteins; D012269:Ribosomal Proteins; D000198:Spectinomycin; D014357:Trypsin