Separation of dermatan sulfate from heparan sulfate in mucopolysaccharidosis urine by chromatography on Sephadex G-75.
Oligosaccharides of testicular hyaluronidase-degraded dermatan sulfate were separated from undegraded dermatan sulfate by chromatography on Sephadex G-75, but not by chromatography on Sephadex G-25. All but the smallest of these oligosaccharides were recovered in excellent yield following dialysis and precipitation with cetyl pyridinium chloride (CPC). G-75 chromatography of dialyzed, concentrated Hunter urine mucopolysaccharides precipitated with CPC resolved most of the large dermatan sulfate into a void volume related peak which was free of heparan sulfate. Decreasing amounts of dermatan sulfate oligosaccharides were eluted with sephadex-retarded polysaccharides, including small amounts which appeared with otherwise pure heparan sulfate.
D002648:Child; D002807:Chondroitin / Q000031:analogs & derivatives*; D002850:Chromatography, Gel / Q000379:methods; D003871:Dermatan Sulfate / Q000302:isolation & purification / Q000652:urine*; D005260:Female; D006025:Glycosaminoglycans / Q000652:urine*; D006497:Heparan Sulfate / Q000302:isolation & purification / Q000652:urine*; D006595:Hexosamines / Q000032:analysis; D006801:Humans; D006821:Hyaluronoglucosaminidase; D007202:Indicators and Reagents; D008297:Male; D009083:Mucopolysaccharidoses / Q000652:urine*; D013053:Spectrophotometry / Q000379:methods; D013464:Sulfuric Acids / Q000032:analysis; D013737:Testis / Q000201:enzymology
D002807:Chondroitin; D003871:Dermatan Sulfate; D006025:Glycosaminoglycans; D006497:Heparan Sulfate; D006595:Hexosamines; D006821:Hyaluronoglucosaminidase; D007202:Indicators and Reagents; D013464:Sulfuric Acids