Abscisic acid studied by fluorescent techniques.
Crude analytical abscisic acid (ABA) exhibited fluorescence (lambdaex 340, lambdaem 425 nm). The results demonstrated a relationship between fluorescence response and ABA concentration, in the range of 0.04-0.38 microgram/ml. Chromatographic purification increased the emission signal, and oxidation induced by adding a sulphuric acid: ethanol (1:1) mixture also increased and displaced it (lambdaex 450, lambdaem 495 nm). Using these properties, a fluorimetric method for identification and evaluation of ABA, had been developed, and used for inhibitor recognition in P. pinea seed coats.
D000040:Abscisic Acid / Q000032:analysis* / Q000302:isolation & purification / Q000494:pharmacology; D001681:Biological Assay; D055598:Chemical Phenomena; D002621:Chemistry; D003509:Cyclohexanecarboxylic Acids / Q000032:analysis*; D012639:Seeds / Q000032:analysis* / Q000187:drug effects; D013050:Spectrometry, Fluorescence
D003509:Cyclohexanecarboxylic Acids; D000040:Abscisic Acid