Purification from brain of an intrinsic membrane protein fraction enriched in (Na+ + K+)-ATPase.
A microsomal fraction from canine brain gray matter has been extracted with the detergent sodium dodecyl sulfate to partially purify the membrane-bound (Na+ + K+)-stimulated adenosine triphosphatase. Phospholipid, glycolipid, and a family of other glycoproteins are also enriched by the procedure; it is proposed that the product is an intrinsic membrane protein fraction. 6--8-fold purification of (Na+ + K+)-ATPase is obtained without solubilizing the enzyme and without irreversibly altering its turnover number. Final specific activities are 350--400 mumol of ATP hydrolyzed/h per mg protein. The stimulation and reversible inactivation of the (Na+ + K+)-ATPase by dodecyl sulfate were examined for information relevant to the mechanism of action of the detergent.
D000251:Adenosine Triphosphatases / Q000302:isolation & purification* / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000201:enzymology*; D001923:Brain Chemistry; D004285:Dogs; D004789:Enzyme Activation; D006017:Glycolipids / Q000302:isolation & purification; D006023:Glycoproteins / Q000302:isolation & purification; D008565:Membrane Proteins / Q000302:isolation & purification*; D008861:Microsomes / Q000201:enzymology; D010743:Phospholipids / Q000302:isolation & purification; D011188:Potassium / Q000378:metabolism; D012964:Sodium / Q000378:metabolism; D012967:Sodium Dodecyl Sulfate
D006017:Glycolipids; D006023:Glycoproteins; D008565:Membrane Proteins; D010743:Phospholipids; D012967:Sodium Dodecyl Sulfate; D012964:Sodium; D000251:Adenosine Triphosphatases; D011188:Potassium