Contribution to the biochemical characterization of cardiac glycosides concerning their influence on ATP-ases.
1. A comparison of the complex binding constants of different glycosides show no significant differences between these different glycosides at least not in the model containing ATP. 2. There are greater differences regarding the ATP-ase activity of the different glycoside complexes measured by means of the Rb-transport method. 3. The absorption of different proscillaridin and methylproscillaridin preparations was investigated with this method. 4. Using the rat as experimental animal the effect of different glycosides on the Na-K and Ca ATP-ase of the heart muscle was demonstrated showing a remarkable activation of the Ca-activated ATP-ase caused by proscillaridin. In order to find an explanation for the therapeutic and toxic effects these investigations have to be repeated in the pig, which shows more similarities in the ATP-ase distribution to man than the rat.
D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002301:Cardiac Glycosides / Q000494:pharmacology*; D066298:In Vitro Techniques; D007700:Kinetics; D009206:Myocardium / Q000201:enzymology; D011188:Potassium / Q000378:metabolism; D051381:Rats; D012964:Sodium / Q000378:metabolism; D013328:Strophanthins / Q000494:pharmacology
D002301:Cardiac Glycosides; D013328:Strophanthins; D012964:Sodium; D000251:Adenosine Triphosphatases; D011188:Potassium; D002118:Calcium