Pulmonary fibrinolytic activity: development of a sensitive radiochromatographic assay.
Pulmonary fibrinolytic activity was determined by a column assay which uses antibovine fibrinogen antibody covalently bound to cyanogen bromide-activated Sepharose 4B and 125I-labeled fibrinogen immunologically bound to the antibody. Fibrinogen-degrading enzyme (plasmin) was passed through the immobilized substrate; the released 1252I-labeled fragments were counted in a gamma-scintillation counter. A positive correlation was found between enzyme concentration and release of fibrinogen degradation products. The assay was sensitive, reproducible and particularly useful for measuring low enzyme activity in dilute bronchopulmonary lavage fluids.
D000818:Animals; D002847:Chromatography, Agarose / Q000379:methods; D006224:Cricetinae; D004800:Enzymes, Immobilized; D005338:Fibrin Fibrinogen Degradation Products / Q000032:analysis; D005340:Fibrinogen / Q000276:immunology; D005341:Fibrinolysin / Q000032:analysis*; D007457:Iodine Radioisotopes; D008168:Lung / Q000201:enzymology*; D007507:Therapeutic Irrigation
D004800:Enzymes, Immobilized; D005338:Fibrin Fibrinogen Degradation Products; D007457:Iodine Radioisotopes; D005340:Fibrinogen; D005341:Fibrinolysin