Control of normal differentiation of myeloid leukemic cells. VI. Inhibition of cell multiplication and the formation of macrophages.
D+ but not D- myeloid leukemic cells can be induced by the appropriate conditioned medium or by serum from endotoxin treated mice, to undergo cell migration in agar, cell attachment to the surface of a Petri dish and differentiation to mature macrophages and granulocytes. Inhibition of cell multiplication by cytosine arabinoside, hydroxyurea, mitomycin C, thymidine, 5-bromodeoxyuridine, 5-iododeoxyuridine, 5-fluorodeoxyuridine or actinomycin D, but not by vinblastine or cycloheximide, induced cell migration, cell attachment to the Petri dish and the formation of macrophages in D+ cells. There was no induction of cell migration or formation of macrophages and a much lower induction of cell attachment in D- cells. The induction of these changes in D+ cells required protein synthesis and the inhibitors showed the same toxicity for D+ and D- cells. The results indicate, that the inhibitors induced specific surface membrane changes in D+ but not in D- cells.
D000818:Animals; D001769:Blood; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002448:Cell Adhesion; D002454:Cell Differentiation; D002455:Cell Division / Q000187:drug effects*; D002465:Cell Movement; D002999:Clone Cells; D003513:Cycloheximide / Q000494:pharmacology; D003561:Cytarabine / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D004731:Endotoxins; D005467:Floxuridine / Q000494:pharmacology; D006918:Hydroxyurea / Q000494:pharmacology; D007065:Idoxuridine / Q000494:pharmacology; D007951:Leukemia, Myeloid / Q000473:pathology*; D008264:Macrophages; D051379:Mice; D008937:Mitomycins / Q000494:pharmacology; D013936:Thymidine / Q000494:pharmacology; D014747:Vinblastine / Q000494:pharmacology
D004731:Endotoxins; D008937:Mitomycins; D005467:Floxuridine; D003561:Cytarabine; D003609:Dactinomycin; D014747:Vinblastine; D003513:Cycloheximide; D001973:Bromodeoxyuridine; D007065:Idoxuridine; D013936:Thymidine; D006918:Hydroxyurea