title: Microheterogeneity of chondroitin sulfates from various cartilages.
abstract: Chondroitin sulfate fractions were isolated from different animal cartilages, including whale, cattle, sheep, ray and shark, by Dowex 1 chromatography followed by ethanol fractionation. Although each preparation showed a single spot when electrophoresed on cellulose acetate, both 4- and 6-sulfated disaccharides were present in chondroitinase digests of each. In particular, the main fraction of bovine tracheal chondroitin sulfate (SO4/Ga1N = 1) gave both the disaccharides in nearly equal amounts, and its IR spectrum showed absorption bands at 820 and 850 cm-1. This fraction yielded three types of tetrasaccharides after digestion with testicular hyaluronidase. Structural studies on these tetrasaccharides, using P. vulgaris chondro-4-sulfatase followed by chondroitinase, showed that one of them is a hybrid consisting of the 4- and 6-sulfated residues. In the light of these facts, a nomenclature for chondroitin sulfates is discussed.
mesh_terms: D000818:Animals; D002356:Cartilage / Q000032:analysis*; D002417:Cattle; D002807:Chondroitin / Q000031:analogs & derivatives*; D002809:Chondroitin Sulfates / Q000032:analysis*; D004187:Disaccharides / Q000032:analysis; D005399:Fishes; D009666:Nose / Q000032:analysis; D012754:Sharks; D012756:Sheep; D012886:Skull / Q000032:analysis; D009626:Terminology as Topic; D014132:Trachea / Q000032:analysis; D014574:Uronic Acids / Q000032:analysis; D014907:Whales
chemical_list: D004187:Disaccharides; D014574:Uronic Acids; D002807:Chondroitin; D002809:Chondroitin Sulfates