title: Comparison of proteoglycans from bovine articular cartilage.
abstract: Four bovine articular cartilages have been compared with regard to the chemical composition of the whole cartilages, the amount of proteoglycan selectively extracted with 3 M MGCl2 or with 3 M guanidine-HCl, and the compositions and physical properties of the isolated proteoglycans. The whole cartilages differ but slightly in composition. Occipital condylar cartilage, a thin cartilage from the smallest joint, contains 4% more collagen and proportionately less proteoglycan than proximal humeral, the thickest cartilage from the largest joint. Each cartilage contains a pool of proteoglycan that resists extraction with 3 M MgCl2 but is extracted with 3 M guanidine-HCl. The proteoglycan extracted from each cartilage with 3 M guanidine-HCl contains a high molecular weight proteoglycan-collagen complex demonstrated by analytical ultracentrifugation and by the turbidity of its visible and ultra-violet spectra. The four cartilages appear to differ most remarkably in the fraction of total proteoglycan extracted from each as proteoglycan-collagen complex.
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000032:analysis*; D002417:Cattle; D003094:Collagen; D006025:Glycosaminoglycans / Q000032:analysis*; D006146:Guanidines; D006811:Humerus; D046911:Macromolecular Substances; D008274:Magnesium; D008970:Molecular Weight; D009777:Occipital Bone; D011485:Protein Binding; D011509:Proteoglycans / Q000032:analysis* / Q000302:isolation & purification; D014457:Ulna
chemical_list: D006025:Glycosaminoglycans; D006146:Guanidines; D046911:Macromolecular Substances; D011509:Proteoglycans; D003094:Collagen; D008274:Magnesium