title: Evidence supporting the identity of beef heart mitochondrial chloroform-released adenosine triphosphatase (ATPase) with coupling factor I.
abstract: Highly purified mitochondrial chloroform-released beef heart ATPase had molecular weight 330 000, five bands (alpha, beta, gamma, delta, epsilon) in sodium dodecyl sulfate gel electrophoresis and could restore the oxidative-phosphorylation function of A particles. Maximal inhibition (90%) of the enzyme by N,N'-dicyclohexylcarbodiimide was achieved at a molar ratio of inhibitor to protein of 30 : 1. Chloroform introduced into an aqueous solution of beef heart coupling factor I protected it from cold inactivation.
mesh_terms: D000251:Adenosine Triphosphatases / Q000302:isolation & purification / Q000378:metabolism; D000818:Animals; D002417:Cattle; D002725:Chloroform; D004579:Electron Transport; D008931:Mitochondria, Muscle / Q000201:enzymology* / Q000378:metabolism; D009124:Muscle Proteins / Q000502:physiology; D009206:Myocardium; D010085:Oxidative Phosphorylation; D010086:Oxidative Phosphorylation Coupling Factors; D011485:Protein Binding
chemical_list: D009124:Muscle Proteins; D010086:Oxidative Phosphorylation Coupling Factors; D002725:Chloroform; D000251:Adenosine Triphosphatases