title: [In vitro effects of aminoglycosides and cephalosporins on rat kidney lysosomes].
abstract: The study of aminoglycoside and cephalosporin antibiotics was performed using a subcellular system in vitro. Lysosomes were purified from rat kidney tissue homogenates by a linear sucrose gradient centrifugation technique. The lysosomal membrane integrity was estimated by measuring the free N-acetyl-beta-D-glycosiminidase activity in the incubation medium. A scale of toxicity was established for eleven aminoglycosides and four cephalosporins.
mesh_terms: D000118:Acetylglucosaminidase / Q000032:analysis; D000617:Aminoglycosides / Q000633:toxicity*; D000818:Animals; D002511:Cephalosporins / Q000633:toxicity*; D066298:In Vitro Techniques; D007668:Kidney / Q000187:drug effects / Q000648:ultrastructure*; D008247:Lysosomes / Q000187:drug effects* / Q000201:enzymology; D008566:Membranes / Q000032:analysis / Q000201:enzymology; D051381:Rats
chemical_list: D000617:Aminoglycosides; D002511:Cephalosporins; D000118:Acetylglucosaminidase
vernacular_title: Effets des aminoglycosides et des céphalosporines sur les lysosomes de rein de rat in vitro.