Preparation and antigenic properties of androsterone-7-BSA conjugate.
The 7-carboxymethoximino derivative of androsterone (1) has been prepared from dehydroisoandrosterone-17-ethyleneketal by a sequence involving inversion at C-3, introduction of a carbonyl at C-7, and reduction of the double bond at C-5. The substance was condensed with BSA by the carbodiimide procedure to afford a conjugate which produced anti-androsterone antiserum in innoculated rabbits. The antiserum is sufficiently active to be useful in radioimmunoassay procedures.
D000738:Androsterone / Q000031:analogs & derivatives / Q000276:immunology*; D000818:Animals; D000941:Antigens; D001681:Biological Assay; D002417:Cattle; D002849:Chromatography, Gas; D003687:Dehydroepiandrosterone; D004305:Dose-Response Relationship, Drug; D008722:Methods; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay; D012710:Serum Albumin, Bovine
D000941:Antigens; D012710:Serum Albumin, Bovine; D003687:Dehydroepiandrosterone; D000738:Androsterone