[Influence of the oxygenation state of the culture media on the level of non esterified fatty acids and of cholesterol in heart cells of the newborn rat].
The uptake of non esterified fatty acids (NEFA), from a medium supplemented with 10% of calf serum, by rat myocardial cells cultured during 8 days, without renewal of the medium, was identical whatever the values of partial pressures of oxygen in the medium. Cellular cholesterol level was reduced when air or oxygen (+5% CO2) was blown into culture bottles; it was enhanced by gassing with nitrogen 24 h later. Cellular NEFA level was reduced by air, but not by oxygen blowing; it was enhanced by gassing with nitrogen 24 h later. The variations observed may originate from pertubations of NEFA beta-oxidation.
D000818:Animals; D000831:Animals, Newborn; D001692:Biological Transport; D002784:Cholesterol / Q000032:analysis / Q000378:metabolism*; D003470:Culture Media; D005230:Fatty Acids, Nonesterified / Q000032:analysis / Q000378:metabolism*; D006321:Heart / Q000187:drug effects; D066298:In Vitro Techniques; D009206:Myocardium / Q000378:metabolism*; D010100:Oxygen / Q000494:pharmacology*; D010313:Partial Pressure; D051381:Rats
D003470:Culture Media; D005230:Fatty Acids, Nonesterified; D002784:Cholesterol; D010100:Oxygen
Influence de l'état d'oxygénation des milieux de culture sur la teneur en acides gras non estérifiés et en cholestérol des cellules cardiaques de rat nouveau-né