Induction of splenomegaly with stimulated leukocyte culture supernatants.
Cell-free medium from stimulated murine leukocyte cultures induced spleen enlargement in vitro. Splenomegaly was measured by [3H]leucine incorporation of spleen fragments in tissue culture. Stimulatory cell-free medium were induced in leukocyte cultures with allogeneic or phytohemagglutinin stimulation. Supernatant activity has lymphokine properties, i.e., cell-free soluble factor(s), generated during specific lymphocyte activation, but which are expressed without reference to H-2 specificity.
D000818:Animals; D002474:Cell-Free System; D005374:Filtration; D007111:Immunity, Cellular; D037102:Lectins / Q000494:pharmacology; D007930:Leucine / Q000378:metabolism; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008970:Molecular Weight; D013154:Spleen / Q000276:immunology / Q000378:metabolism; D013163:Splenomegaly / Q000276:immunology*
D037102:Lectins; D007930:Leucine