T-lymphocyte response to H-2 mutants. I. Proliferation is dependent on Ly 1+2+ cells.
We have determined the Ly phenotype of the T lymphocytes which proliferate in response to mutant H-2K and H-2D alloantigens in primary mixed lymphocyte culture. Responder T cells proliferating in reciprocal cultures of H-2d(KdDd) and H-2da(KdDda) lymphocytes were typed Ly 2+ through selective depletion with specific alloantiserum plus complement. Further, B6-Ly 1a lymphocytes proliferating in response to B6-H-2ba and B6-H-2bf stimulators were typed as Ly 1+2+ through similar analysis. These results are discussed with regard to their impact on views of lymphocyte differentiation and factors determining the identity of alloreactive lymphocytes.
D000818:Animals; D000941:Antigens; D003601:Cytotoxicity Tests, Immunologic; D006183:H-2 Antigens; D007106:Immune Sera; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D009154:Mutation; D010641:Phenotype; D013601:T-Lymphocytes / Q000276:immunology*
D000941:Antigens; D006183:H-2 Antigens; D007106:Immune Sera