[Ultrastructure of the particles reconstituted by complementation of extracts from chl-r mutants of Escherichia coli K 12].
By freeze-fracturing it is shown that the vesicles reconstituted by complementation of the chlA and chlB mutants of E. coli K 12 extracts are characterized by an asymmetric membrane bilayer. In a feature quite similar to the original intact plasma membranes, the membrane splits in two halves and the intramembranous particles are asymmetrically distributed on the two facture faces. It is proposed that the process of membrane reconstitution, which is also associated with the restoration of nitrate-reductase activity, relies on a sequence of increasing complexity of the molecular organisation.
D000251:Adenosine Triphosphatases; D002459:Cell Fusion; D002462:Cell Membrane / Q000201:enzymology / Q000648:ultrastructure*; D002704:Chlorates; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000378:metabolism*; D005614:Freeze Fracturing; D008563:Membrane Lipids; D008565:Membrane Proteins; D009154:Mutation; D009565:Nitrate Reductases; D011487:Protein Conformation; D013347:Subcellular Fractions / Q000648:ultrastructure
D002704:Chlorates; D008563:Membrane Lipids; D008565:Membrane Proteins; D009565:Nitrate Reductases; D000251:Adenosine Triphosphatases
Ultrastructure des particules reconstituées par complémentation d'extraits de mutants chl-r d'Escherichia coli K 12