title: Giant cell tumor of bone. Fine structural localization of alkaline phosphatase.
abstract: The fine structural localization of nonspecific alkaline phosphatase was elucidated in two giant cell tumors of bone using lead as capturing ion and beta-glycerophosphate as substrate in the incubation solution. Lead phosphate precipitate--indicating presence of alkaline phosphatase--was demonstrated on the plasma membranes, and the membranes bordering vesicles and vacuoles of presumed endocytotic nature, in giant cells and type 1 stromal cells (fibroblast-like cells). The findings support the view that stromal cells type I and giant cells are histogenetically related.
mesh_terms: D000328:Adult; D000469:Alkaline Phosphatase / Q000032:analysis*; D001859:Bone Neoplasms / Q000201:enzymology* / Q000648:ultrastructure; D002462:Cell Membrane / Q000201:enzymology; D004705:Endocytosis; D005260:Female; D005870:Giant Cell Tumors / Q000201:enzymology* / Q000648:ultrastructure; D006801:Humans; D008297:Male; D008854:Microscopy, Electron; D014617:Vacuoles / Q000201:enzymology
chemical_list: D000469:Alkaline Phosphatase