Re-investigation of the effects of L-glutamine and L-asparagine on the Neurospora crassa NADP-specific glutamate dehydrogenase.
L-Glutamine, when purified free of traces of NH4+ present in solution, does not act as an alternative substrate to NH4+ for the NADP-specific glutamate dehydrogenase of Neurospora. L-Glutamine interferes with detection of small quantities of NH4+ by Nessler's reagent. L-Asparagine is not an alternative substrate to NH4+ for this enzyme.
D001216:Asparagine / Q000378:metabolism*; D002852:Chromatography, Ion Exchange; D005969:Glutamate Dehydrogenase / Q000378:metabolism*; D005973:Glutamine / Q000378:metabolism*; D009491:Neurospora / Q000201:enzymology*; D009492:Neurospora crassa / Q000201:enzymology*; D000644:Quaternary Ammonium Compounds / Q000378:metabolism
D000644:Quaternary Ammonium Compounds; D005973:Glutamine; D001216:Asparagine; D005969:Glutamate Dehydrogenase