[Simultaneous estimation of flow rates, electrolyte and proteinconcentration and discelectrophoretic separation of fractionated human parotid saliva(author's transl)].
Fractionated human parotid saliva from normal persons was separated by column-acryamid-gel discelectrophoresis. Flow rates, electrolyte-concentrations and total protein content of the stimulated and unstimulated parotid-secretion were determined simultaneously. Significant patterns of proteincomposition were found for stimulated and unstimulated parotid saliva. Most typical changes of protein bands were found in the anodal near gel region, within a group of four bands, called by us f, g, h and i. In the cathodal near gel region as well significant changes in the bands a, b, c and d were found; by glycoprotein staining these bands showed good staining.
D000293:Adolescent; D000328:Adult; D002118:Calcium / Q000032:analysis; D003720:Densitometry; D004573:Electrolytes / Q000032:analysis; D004589:Electrophoresis, Disc; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D008297:Male; D010306:Parotid Gland / Q000378:metabolism*; D011188:Potassium / Q000032:analysis; D011506:Proteins / Q000032:analysis; D012463:Saliva / Q000032:analysis*; D012636:Secretory Rate; D012964:Sodium / Q000032:analysis
D004573:Electrolytes; D011506:Proteins; D012964:Sodium; D011188:Potassium; D002118:Calcium
Vergleichende Untersuchungen von Flussraten, elektrolyt- und Gesamteiweisskonzentrationen und diskelektrophoretische Auftrennung des Sekretes an fraktioniert gewonnenem menschli