[Studies on the complete enzymatic hydrolysis of steroid conjugates in urine (author's transl)].
The incubation of urine with beta-glucuronidase/arylsuphatase from Helix pomatia results in the incomplete hydrolysis of urinary steroid conjugates, because inhibitors of these enzymes are present in urine; The inhibitors can be separated from the steroid conjugates by chromatography of the urine on a column of Amberlite XAD-2. By this method a complete enzymatic hydrolysis of urinary steroid conjugates is possible in 24 hours at 37 degrees C or in 2 hours at 55 degrees C.
D000738:Androsterone / Q000652:urine; D001192:Arylsulfatases / Q000037:antagonists & inhibitors; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D003687:Dehydroepiandrosterone / Q000652:urine; D004791:Enzyme Inhibitors / Q000652:urine; D004964:Estriol / Q000652:urine; D005043:Etiocholanolone / Q000652:urine; D005260:Female; D005966:Glucuronidase / Q000037:antagonists & inhibitors; D006372:Helix, Snails; D006801:Humans; D006868:Hydrolysis; D011247:Pregnancy; D011276:Pregnanediol / Q000652:urine; D011279:Pregnanetriol / Q000652:urine; D013256:Steroids / Q000652:urine*
D004791:Enzyme Inhibitors; D013256:Steroids; D011279:Pregnanetriol; D003687:Dehydroepiandrosterone; D005043:Etiocholanolone; D000738:Androsterone; D001192:Arylsulfatases; D005966:Glucuronidase; D004964:Estriol; D011276:Pregnanediol
Untersuchungen zur vollständigen enzymatischen Hydrolyse von Steroidkonjugaten im Harn