The effects of glycosaminoglycans on surface-mediated interactions of lymphoid cells.
The effect of chondroitin sulphate (CS) was studied in two cell surface-mediated interactions, the complement-independent contact reaction and antibody-induced capping of H-2 antigens. CS inhibited contact cytotoxicity when present in the reaction medium, but preincubation in CS of either killer cells or target cells had no effect. The capacity of CS-pretreated target cells to absorb H-2 antibodies was not reduced. However, a similar pretreatment resulted in an increased readiness to capping as induced by H-2 antibody followed by an anti-mouse immunoglobulin; in control cells time taken for the same percentage of caps to be formed was considerably longer than in CS-pretreated cells. A possible mechanism of this CS effect and the general biological role of glycosaminoglycans (particularly in immune interactions) is discussed.
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D002809:Chondroitin Sulfates / Q000494:pharmacology; D003165:Complement System Proteins; D003601:Cytotoxicity Tests, Immunologic; D006025:Glycosaminoglycans / Q000494:pharmacology*; D006649:Histocompatibility Antigens; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL / Q000276:immunology
D006025:Glycosaminoglycans; D006649:Histocompatibility Antigens; D002809:Chondroitin Sulfates; D003165:Complement System Proteins