[Preservation of rat liver using prolonged perfusion with a complex solution lacking hemoglobin].
The author's aim was to investigate conservation of rat liver by continuous perfusion of the isolated organ in conditions of normal temperature, as well as in hypothermia. A complex solution was used, lacking hemoglobin. Over a period of 5 hours of conservation the authors evaluated the perfusion rate, the concentration of lactate and pyruvate in the conservation medium, while on histological preparations was investigated the formation of fibrin deposits in the intra-hepatic vessels. The results confirmed the positive effects of hypothermia, that is not due exclusively to a limiting of the metabolic necessities of the organ but also to a reduction of the fibrinogenesis and a slowing down of the formation of intra-hepatic microaggregates.
D000818:Animals; D003911:Dextrans; D004573:Electrolytes; D005947:Glucose; D007035:Hypothermia; D008099:Liver; D016031:Liver Transplantation; D009926:Organ Preservation; D010477:Perfusion / Q000379:methods*; D051381:Rats; D012996:Solutions; D014021:Tissue Preservation; D014184:Transplantation, Homologous
D003911:Dextrans; D004573:Electrolytes; D012996:Solutions; D005947:Glucose
Conservarea ficatului de şobolan prin perfuzare prelungită cu o soluţie complexă lipsită de hemoglobină