In vivo transfer of the ti-plasmid of Agrobacterium tumefaciens to Escherichia coli.
The Ti-plasmids are naturally self-transmissible from their normal host Agrobacterium to E. coli. They are however unable to stably establish themselves as a replicon in E. coli. It is nevertheless possible to study the Ti-plasmids in E. coli with the help of Ti::RP4 cointegrate plasmids that transfer and maintain themselves very efficiently in E. coli. An E. coli harbouring such a Ti::RP4 plasmid is unable to catabolize octopine and unable to induce crown-gall tumours on plants.
D003227:Conjugation, Genetic; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000235:genetics*; D010641:Phenotype; D010957:Plasmids; D012231:Rhizobium / Q000235:genetics; D012293:Rifampin / Q000494:pharmacology; D000198:Spectinomycin / Q000494:pharmacology; D013307:Streptomycin / Q000494:pharmacology; D014169:Transformation, Bacterial
D000198:Spectinomycin; D012293:Rifampin; D013307:Streptomycin