Localization of the interference of ascorbic acid deficiency with bile acid biogenesis.
The catabolism of 26-14C-cholesterol and of 26-14C-7alpha-hydroxycholesterol, the first stage in the transformation of cholesterol to bile acids, was studied in guinea-pigs with chronic latent vitamin C deficiency. Vitamin C deficiency markedly inhibited the oxidation of 26-14C-cholesterol to 14CO2, but did not significantly affect the catabolism of 26-14C-7alpha-hydroxycholesterol. The distribution of 14C in the tissues and body fluids of control and vitamin-deficient guinea pigs injected with labelled 7alpha-hydroxycholesterol was likewise the same. Ascorbic acids is probably needed only for 7alpha-hydroxylation of cholesterol, while the other stages of bile acid biogenesis are independent of vitamin C.
D000818:Animals; D001206:Ascorbic Acid Deficiency / Q000378:metabolism*; D001647:Bile Acids and Salts / Q000096:biosynthesis*; D001711:Biotransformation; D002784:Cholesterol / Q000378:metabolism; D002855:Chromatography, Thin Layer; D006168:Guinea Pigs; D006888:Hydroxycholesterols / Q000378:metabolism; D006900:Hydroxylation; D008297:Male
D001647:Bile Acids and Salts; D006888:Hydroxycholesterols; D002784:Cholesterol