title: Synchronizing temperatures and rRNA metabolism in Tetrahymena pyriformis.
abstract: 1. In Tetrahymena pyriformis pre-rRNA is synthesized and efficiently processed and translocated into the cytoplasm at both a supraoptimal (34 degrees C) and a suboptimal (8 degrees C) synchronizing temperature. 2. At the heat shock temperature (34 degrees C) no substantial differences in the kinetics of the main intracellular events of rRNA-metabolism compared to the optimal growth temperature (28 degrees C) were found. 3. The high temperature, however, induces a strong retardation of uptake of [3H]adenosine into the cells and a reduction of the cellular ATP pool size. 4. At the cold shock temperature (8 degrees C) the rates of transcription and nucleocytoplasmic transport of rRNA as well as of nucleotide pool equilibration are reduced to a similar extent (25-30% of the optimal rates at 28 degrees C). 5. The results are discussed and compared with the effects of sub- and supraoptimal temperatures on rRNA synthesis and processing in mammalian cells.
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D008861:Microsomes / Q000032:analysis; D012335:RNA, Ribosomal / Q000032:analysis / Q000378:metabolism*; D013696:Temperature; D013769:Tetrahymena pyriformis / Q000378:metabolism*; D013997:Time Factors; D014529:Uridine / Q000378:metabolism; D014544:Uridine Triphosphate / Q000096:biosynthesis
chemical_list: D012335:RNA, Ribosomal; D000255:Adenosine Triphosphate; D014544:Uridine Triphosphate; D014529:Uridine