title: [Culture of cells isolated from the smooth muscle of the rat duodenum].
abstract: A culture obtained from rat duodenal smooth muscle layer is described. The cells are isolated by trypsinization (0.2 %) and the medium used for culture is either MEM with glutamine and non essentiel AA, or RPMI, both containing 10 % foetal calf serum. The cell culture contains both smooth muscle cells and fibroblasts in proportions varying with the age of the culture. At day 6, cell differenciation is important. At day 12, when the cells and confluent, the majority of the cells are fibroblasts. Although it is difficult, the transfer of cells is possible at least twice.
mesh_terms: D000818:Animals; D002478:Cells, Cultured / Q000166:cytology; D003470:Culture Media; D004386:Duodenum / Q000166:cytology*; D006652:Histological Techniques; D009130:Muscle, Smooth / Q000166:cytology*; D051381:Rats; D014357:Trypsin
chemical_list: D003470:Culture Media; D014357:Trypsin
vernacular_title: Culture de cellules isolées du muscle lisse de duodénum de rat.