Genetic identification of a locus linked to the rat MHC that codes for a membrane antigen detectable with cytotoxic lymphocytes.
Cell-mediated cytolytic (CMC) responses resulted from immunizations between rat strains purported to be identical at Ag-B, the major histocompatibility complex (MHC), but differing at other loci not linked to Ag-B. In vivo-priming followed by secondary in vitro stimulation was required to generate a measurable CMC response as determined by a 51Cr-release assay. Neither in vivo nor in vitro stimulation alone was adequate. The CMC responses generated in a strain combination considered Ag-B identical (LEW . B3:BN) were specific for a determinant controlled by a gene linked to Ag-B, which has been designated Ag-L. The CMC response appears not to be restricted to sygeneity at Ag-B. In addition, the data presented demonstrate a recombinant between Ag-B and Ag-L, and suggest that the gene has failed to transfer with the MHC during the isolation of the LEW . B3 and F-344 . B3 congenic strains.
D000818:Animals; D000954:Antigens, Surface / Q000235:genetics*; D001667:Binding, Competitive; D002874:Chromosome Mapping; D003602:Cytotoxicity, Immunologic; D006649:Histocompatibility Antigens / Q000235:genetics; D007106:Immune Sera / Q000494:pharmacology; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000276:immunology*; D008285:Major Histocompatibility Complex; D051381:Rats; D011912:Rats, Inbred ACI; D011914:Rats, Inbred BN; D011915:Rats, Inbred BUF; D011916:Rats, Inbred F344; D011917:Rats, Inbred Lew; D011995:Recombination, Genetic; D016038:Skin Transplantation
D000954:Antigens, Surface; D006649:Histocompatibility Antigens; D007106:Immune Sera