Transplantation in miniature swine. II. In vitro parameters of histocompatibility in MSLA homozygous minipigs.
A selective breeding program based on serological techniques was used to establish three herds of miniature swine, each homozygous for a different allele at the major histocompatibility complex, termed MSLA. By selective immunization of these swine, high-titered antisera detecting the products of individual MSLA alleles were raised and used to confirm the genotype of offspring in these herds. Techniques for performing one-way mixed leukocyte cultures in these pigs were developed and demonstrated the presence of a strong mixed leukocyte culture locus (or loci) closely linked to the serologically defined MSLA locus. Cell-mediated cytotoxicity assays based on 51Cr release were developed to monitor the course of cellular immunity following allotransplantation and exhibited specificity identical to that predicted by the serological typing.
D000818:Animals; D003601:Cytotoxicity Tests, Immunologic; D006649:Histocompatibility Antigens / Q000032:analysis; D006650:Histocompatibility Testing; D006720:Homozygote; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000276:immunology; D016038:Skin Transplantation; D013552:Swine / Q000276:immunology*; D014181:Transplantation Immunology; D014184:Transplantation, Homologous
D006649:Histocompatibility Antigens