[Size comparison of poly (A)-RNA from polysomes and from nuclei of the yeast Saccharomyces cerevisiae (author's transl)].
The size of poly (A)-RNA from polysomes and cell nuclei of the yeast Saccharomyces cerevisiae was investigated. Pulslabelled cells ([14C] adenin) were cracked by the French press; polysomes and nuclei were separately isolated and the RNA was finally extracted with phenol. The separation of poly (A)-containing and poly (A)-lacking fractions was achieved by oligo (dT) cellulose. These fractions were characterized by sedimentation analysis. The main portion of polysomal poly (A)-RNA sedimented with a rate of 8 to 14S, whereas the poly (A)-RNA of nuclei exhibited a sedimentation rate of 12 to 17S. Thus nuclear poly (A)-RNA is about 20-30% larger than polysomal poly (A)-RNA.
D002467:Cell Nucleus / Q000032:analysis*; D008970:Molecular Weight; D011061:Poly A / Q000032:analysis*; D011132:Polyribosomes / Q000032:analysis*; D012313:RNA / Q000032:analysis*; D012335:RNA, Ribosomal / Q000032:analysis; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
D012335:RNA, Ribosomal; D011061:Poly A; D012313:RNA
Grössenvergleich der Poly (A)-RNA aud Polysomen und aus Kernen bei der Hefe Saccharomyces cerevisiae