Genetic control of responses to bacterial lipopolysaccharides in mice. II. A gene that influences a membrane component involved in the activation of bone marrow-derived lymphocytes by lipipolysaccharides.
C3H/HeJ mice contain a defect in a single autosomal locus which is not linked to the H-2 histocompatibility or the heavy chain allotype loci that restrict immune, mitogenic, and polyclonal responses to bacterial lipopolysaccharides (LPS). Adult thymectomized C3H/HeJ mice that have been irradiated and reconstituted with C3HeB/FeJ bone marrow cells respond well to LPS. Cell-mixing experiments using C3H/HEJ-C3HeB/FeJ spleen cultures show that the failure of C3H/HeJ spleen cells to support responses to LPS is not due to nonspecific or LPS-induced suppressive events, or the lack of accessory cell types. C3H/HeJ and C3HeB/FeJ spleen cells bind LPS and respond to other B cell mitogens equally well. We suggest that the B lymphocytes of C3H/HeJ mice have a defect in a membrane component that is activated via interaction with LPS, and initiates the intracellular events that lead to cell proliferation.
D000818:Animals; D001666:Binding Sites, Antibody; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D001894:Borohydrides / Q000378:metabolism; D002462:Cell Membrane / Q000276:immunology*; D003911:Dextrans; D004926:Escherichia coli / Q000276:immunology; D006462:Hemolytic Plaque Technique; D006649:Histocompatibility Antigens; D007125:Immunogenetics; D008070:Lipopolysaccharides / Q000276:immunology*; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D017710:Lymphocyte Transfusion; D051379:Mice; D008809:Mice, Inbred C3H; D011070:Poly I-C; D011135:Polysaccharides, Bacterial; D011828:Radiation Chimera; D013934:Thymectomy; D013936:Thymidine / Q000378:metabolism; D013950:Thymus Gland / Q000166:cytology; D014184:Transplantation, Homologous; D014316:Tritium; D014373:Tuberculin
D001894:Borohydrides; D003911:Dextrans; D006649:Histocompatibility Antigens; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D014373:Tuberculin; D014316:Tritium; D011070:Poly I-C; D013936:Thymidine