[Radioimmunologic determination of the thyrotropin releasing hormone].
We describe the preliminary steps for a radio-immunoassay of Thyrotropin Releasing Hormone (TRH). Rabbit antiserum at dilution 1 : 10 000 is used with radioiodinated TRH (125I). We are able to assay from 5 to 1 000 pg unlabeled TRH with an intraassay reporducibility varying from 7 to 4 % and the lowest detectable amount in this system is 10 pg TRH. TRH mean and standard deviation in normal subjects are 136,9 and 25,3 pg/ml.
D005260:Female; D006801:Humans; D008297:Male; D011863:Radioimmunoassay; D013973:Thyrotropin-Releasing Hormone / Q000097:blood*
D013973:Thyrotropin-Releasing Hormone
Dosage radioimmunologique de la thyrotropin releasing hormone.