Chalone-like effect abrogated by dextran sulphate and heparin polyanion pretreatment of target cells.
Chalone prepared from primary BALB/c mouse embryo fibroblasts caused a 33% reduction in incorporation of tritiated thymidine in the cultures of the second in vitro passage of BALB/c embryo fibroblasts, whereas chalone prepared from thymus, skin and spleen was without effect. Pretreatment of BALB/c secondary fibroblasts with the polyanions dextran sulphate and heparin abrogated the chalone effect. The polycations DEAE-dextran and polybrene were without effect. The effect of incubation with the dextran sulphate polyanion was reversed when followed by incubation with DEAE-dextran polycation.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D003864:Depression, Chemical; D003911:Dextrans / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D006131:Growth Inhibitors / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D006493:Heparin / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D012867:Skin; D013154:Spleen; D013936:Thymidine / Q000378:metabolism; D013950:Thymus Gland
D003911:Dextrans; D006131:Growth Inhibitors; D006493:Heparin; D004247:DNA; D013936:Thymidine