Examination of stomach wall Mg2+-Na+-K+-dependent ATPase, ATP, and ADP in pylorus-ligated rats.
Stomach wall Mg2+-Na+-K+-dependent ATPase, ATP, and ADP were studied in rats, at 4, 7, 17, and 24 hr after pylorus ligation, in order to relate H+ secretion and ulcer development. It was observed, in both parts of forestomach and corpus + antrum, that the stomach wall Mg2+-Na+-K+-dependent ATPase significantly increased at 4 and 7 hr, while it significantly decreased at 24 hr, after pylorus ligation. Contrary to this, stomach-wall ATP decreased at 4 hr, relatively increased at 7 hr, thereafter significantly decreased in forestomach and corpus + antrum. Ouabain inhibited the H+ secretion, ulcer development, and stomach wall Mg2+-Na+-K+-dependent ATPase activity. The possible causal interrelationships between the examined parameters are studied.
D000244:Adenosine Diphosphate / Q000378:metabolism*; D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D003864:Depression, Chemical; D004195:Disease Models, Animal; D005260:Female; D005745:Gastric Acidity Determination; D005750:Gastric Juice / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism*; D008026:Ligation; D008274:Magnesium; D008297:Male; D010042:Ouabain / Q000494:pharmacology; D011188:Potassium; D011708:Pylorus / Q000601:surgery; D051381:Rats; D012964:Sodium; D013270:Stomach / Q000201:enzymology; D013276:Stomach Ulcer / Q000209:etiology
D010042:Ouabain; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D012964:Sodium; D000251:Adenosine Triphosphatases; D008274:Magnesium; D011188:Potassium