Studies on steroids. III. A new type of derivative for electron capture-gas chromatography of ketosteroids.
Pentafluorobenzyloxyamine is presented as a new derivatizing agent for gas chromatography of ketones using electron capture detection. The typical 17-ketosteroid was readily transformed into the O-pentafluorobenzyloxime which on usual trimethylsilylation led to a 3-trimethylsilyl ether derivative exhibiting good gas chromatographic properties. The derivatization procedure was applied to the determination of dehydroepiandrosterone in human plasma by electron capture-gas chromatography employing an internal standard method and in consequence satisfactory results were obtained.
D002849:Chromatography, Gas / Q000379:methods; D003687:Dehydroepiandrosterone / Q000097:blood; D006801:Humans; D007202:Indicators and Reagents; D007664:Ketosteroids / Q000032:analysis*; D010091:Oximes; D014297:Trimethylsilyl Compounds
D007202:Indicators and Reagents; D007664:Ketosteroids; D010091:Oximes; D014297:Trimethylsilyl Compounds; D003687:Dehydroepiandrosterone