Glycosaminoglycans and proteoglycans of human chondrosarcoma.
The glycosaminoglycans and proteoglycans of a human chondrosarcoma have been studied. Glycosaminoglycans were fractionated and identified by cetylpirdium chloride (CPC) cellulose chromatography, ECTEOLA cellulose ion-exchange chromatography and electrophoresis on cellulose acetate. Proteoglycans were extracted by low ionic strength solutions and by 4 M guanidinium chloride and fractioned by equilibrium density-gradient centrifugation and gel chromatography on Sepharose 2B. The tumour matrix contained both the 4- and 6-sulphate isomers of chondroitin sulphate and a high concentration (12% of hexosamine) of hyaluronic acid. Proteoglycans were poor in carbohydrate moieties and proportion were capable of aggregation. Amino acid analysis of the fractionated proteoglycans suggested the presence of a single protein core. A substance with the characteristic amino acid composition of glycoprotein link was recovered from the top of the dissociative density gradient.
D000596:Amino Acids / Q000032:analysis; D002813:Chondrosarcoma / Q000378:metabolism*; D005688:Galactosamine / Q000032:analysis; D005944:Glucosamine / Q000032:analysis; D006025:Glycosaminoglycans / Q000302:isolation & purification / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D010206:Papain; D011509:Proteoglycans / Q000302:isolation & purification / Q000378:metabolism; D013055:Spectrophotometry, Infrared; D014574:Uronic Acids / Q000032:analysis
D000596:Amino Acids; D006025:Glycosaminoglycans; D011509:Proteoglycans; D014574:Uronic Acids; D005688:Galactosamine; D010206:Papain; D005944:Glucosamine