title: Reproducibility of vitamin assays with mutants of Neurospora crassa maintained by freeze-drying.
abstract: Three mutant strains (ATCC 9277, ATCC 9278, ATCC 9683) of Neurospora crassa requiring choline, inositol, and p-aminobenzoic acid respectively for normal growth were deposited at the ATCC by G.W. Beadle 30 years ago and were preserved at various time intervals by freeze-drying. Each preservation batch yielded cultures that, when used for biological assays, exhibited the same biochemical properties as they originally possessed. The freeze-drying technique is shown to be applicable to preserving biological properties of sporulating fungi for bioassay over prolonged periods of time.
mesh_terms: D001681:Biological Assay; D002794:Choline / Q000378:metabolism*; D005612:Freeze Drying; D007294:Inositol / Q000378:metabolism*; D009154:Mutation; D009491:Neurospora / Q000378:metabolism*; D009492:Neurospora crassa / Q000378:metabolism*
chemical_list: D007294:Inositol; D002794:Choline