Aromatizing activity of placental microsomal fractions from ewes in late gestation.
Placental microsomes from eight domestic sheep at 136-146 days of gestation were incubated with radioactive androstenedione, testosterone and dehydroepiandrosterone. Aromatizing activity was examined in the presence and absence of cortisol and the rates of both oestrone and oestradiol synthesis were measured. Oestrone predominated in preference to oestradiol in most of the incubations, a result opposite to that found with human placentae. The sharp increase in the rate of oestradiol production found in the 144- to 146-day-old placentae incubated with testosterone may indicate a more rapid increase of aromatizing than of 17beta-hydroxysteriod dehydrogenase activity. The presence of cortisol in the mixtures did not significantly affect the placental aromatizing activity, indicating that there is no direct effect of cortisol on the enzyme system as measured in vitro. The dramatic rise of overall mean aromatizing activity from 4.86 plus or minus 0.22 (S.E.M.) at 138-141 days of gestation to 12.96 plus or minus 0.38 pmol/mg protein/min at 144-146 days (with a greater relative increase in the rate of oestradiol formation), suggests that changes in placental aromatizing activity may play an important role in maternal and foetal plasma oestrogen surges before ovine parturition.
D000735:Androstenedione / Q000378:metabolism; D000818:Animals; D002850:Chromatography, Gel; D003687:Dehydroepiandrosterone / Q000378:metabolism; D004958:Estradiol / Q000096:biosynthesis*; D004970:Estrone / Q000096:biosynthesis*; D005260:Female; D006854:Hydrocortisone / Q000494:pharmacology*; D066298:In Vitro Techniques; D008861:Microsomes / Q000378:metabolism*; D010920:Placenta / Q000378:metabolism* / Q000648:ultrastructure; D011247:Pregnancy; D011270:Pregnancy, Animal; D012756:Sheep / Q000502:physiology*; D013347:Subcellular Fractions / Q000378:metabolism; D013739:Testosterone / Q000378:metabolism; D013997:Time Factors
D004970:Estrone; D013739:Testosterone; D000735:Androstenedione; D003687:Dehydroepiandrosterone; D004958:Estradiol; D006854:Hydrocortisone