[Perfusion of isolated rat liver. Utilization of a perfusion without heparin. Validity of the preparation].
The blood and liver giver animals are given "Tromexan" for a period of three days. The blood defibrination is finished off by shaking. The perfusion is made of defibrinated blood to which is added a compounding of mineral salts, albumin and glucose in adequate proportions. The fluid is used during 150 minutes with a biliary flow of 4 ul/minute (instead of 8 in perfusions with heparine). The validity of the preparation is tested by biliary acids, cholesterol and Brom Sulfon Phtalein biliary elimination research. This fluid can be used when heparin is avoided: for example in a case of investigation of fat emulsion scrubbing.
D000818:Animals; D001647:Bile Acids and Salts / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism; D006493:Heparin; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism*; D010477:Perfusion / Q000379:methods; D051381:Rats
D001647:Bile Acids and Salts; D006493:Heparin; D002784:Cholesterol
Perfusion du foie de rat isolé. Utilisation d'un perfusat sans héparine. Validité de la préparation.