Size restriction on utilization of peptides by amino acid auxotrophs of Neurospora crassa.
Growth of an amino acid auxotroph of Neurospora crassa on oligopeptides is shown to occur by extracellular hydrolysis, with subsequent utilization of monomer amino acid residues, and by transport of peptides. Peptides with a hydrodynamic volume greater than that of trileucine are not transported, and this lack of transport is shown to be due to restriction by the oligopeptide transport system rather than the cell wall.
D000596:Amino Acids / Q000378:metabolism*; D001693:Biological Transport, Active; D002474:Cell-Free System; D005591:Chemical Fractionation; D010450:Endopeptidases / Q000378:metabolism; D006868:Hydrolysis; D007930:Leucine / Q000378:metabolism; D009154:Mutation; D009491:Neurospora / Q000378:metabolism*; D009492:Neurospora crassa / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D009842:Oligopeptides / Q000378:metabolism*
D000596:Amino Acids; D009842:Oligopeptides; D010450:Endopeptidases; D007930:Leucine