The role of glutathione in renal cortical tissue. Effects of diamide on Na+ and GSSG levels, amino acid transport and Na+-K+-ATPase activity.
The effects of diamide were studied in rat kidney cortical tissue. It was found that diamide increased oxidized glutathione levels and inhibited Na+-K+-ATPase activity. Consistent with this finding was the observation that diamide compromised the sodium gradients maintained in renal cortical slices. Amino acid transport studies with ouabain or a sodium-free buffer indicated that diamide interferes with both Na+-dependent and Na+-independent transport systems. These results indicate that diamide has a number of different effects on renal cortical tissue and emphasize the important role of glutathione in maintaining control of a number of key metabolic pathways.
D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors*; D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D001391:Azo Compounds / Q000494:pharmacology*; D002352:Carrier Proteins / Q000378:metabolism; D003958:Diamide / Q000494:pharmacology*; D005978:Glutathione / Q000031:analogs & derivatives / Q000378:metabolism*; D066298:In Vitro Techniques; D007672:Kidney Cortex / Q000187:drug effects / Q000378:metabolism*; D010084:Oxidation-Reduction; D011188:Potassium / Q000378:metabolism; D047428:Protein Kinase Inhibitors; D051381:Rats; D012964:Sodium / Q000378:metabolism*
D000596:Amino Acids; D001391:Azo Compounds; D002352:Carrier Proteins; D047428:Protein Kinase Inhibitors; D003958:Diamide; D012964:Sodium; D000251:Adenosine Triphosphatases; D005978:Glutathione; D011188:Potassium