[Effect of SH-reagents on ATPase systems of rabbit skeletal muscle nuclei].
The influence of sulfhydryl reagents on ATPase systems of rabbit sceletal muscles nuclei was studied. It is found that p-ChMB at low concentration similarly inhibits both Mg2+- and Mg2+, Ca2+-ATPases. p-ChMB at higher concentrations inhibits completely Mg2+, Ca2+-ATPase, while Mg2+- ATPase--only by 60%. N-EM is lesser specific inhibitor of SH-groups, than p-ChMB. The degree of nuclear ATPases inhibition by N-EM is practically identical. Using inhibitory analysis, two hypes of skeletal muscles nuclei SH-groups are found: easily reacting with N-EM, and those reacting with N-EM at more high concentrations, which are essential for ATPase ATP-hydrolysing activity. ATP defends Mg2+, Ca2+-ATPase, but not the Mg2+-ATPase from N-EM inhibitory action. Cysteine completely eliminates the inhibitory effect of p-ChMB on Mg2+-ATPase but only 40% on MG2+, Ca2+-ATPase. Mg2+, Ca2+-ATPase of nuclei is more sensitive to the sulfhydryl venoms action than Mg2+-ATPase.
D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002467:Cell Nucleus / Q000201:enzymology; D002729:Chloromercuribenzoates / Q000494:pharmacology; D005033:Ethylmaleimide / Q000494:pharmacology; D008274:Magnesium / Q000494:pharmacology; D009132:Muscles / Q000201:enzymology*; D011817:Rabbits; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013439:Sulfhydryl Reagents / Q000494:pharmacology*
D002729:Chloromercuribenzoates; D013438:Sulfhydryl Compounds; D013439:Sulfhydryl Reagents; D000251:Adenosine Triphosphatases; D008274:Magnesium; D005033:Ethylmaleimide; D002118:Calcium
Bliianie sul'fgidril'nykh reagentov na ATPaznye sistemy iader skelitnykh myshts krolikov.