title: Accumulation of platelets at sites of antigen-antibody-mediated injury: a possible role for IgE antibody and mast cells.
abstract: Circulating 51Cr-labeled platelets accumulate at skin sites in which a reversed passive Arthus reaction has been induced. The accumulation is biphasic in time and is accompanied by an increased vascular permeability. Increased permeability itself, however, will not produce localization of platelets. A similar platelet accumulation was observed upon injection of compound 48/80 or anti-IgE antibody into the skin and this was not altered in rabbits depleted of complement or neutrophils. Activation of skin mast cells and release of a platelet-activating factor (PAF) is suggested as a mechanism for the effect produced by anti-IgE and compound 48/80. The first phase of platelet accumulation in the Arthus reaction was also unaffected in rabbits depleted of neutrophils or complement, which may suggest a role for IgE antibody and mast cells. The second phase of accumulation was diminished in complement-depleted animals and abrogated in rabbits without neutrophils, suggesting a complement and neutrophil-mediated process but which still might be mediated through mast cell activation by neutrophil cationic protein.
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D000936:Antigen-Antibody Complex; D001183:Arthus Reaction / Q000276:immunology*; D001792:Blood Platelets / Q000276:immunology*; D002465:Cell Movement; D003165:Complement System Proteins; D007073:Immunoglobulin E; D007074:Immunoglobulin G; D008407:Mast Cells / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology; D011817:Rabbits; D012709:Serum Albumin / Q000276:immunology
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000936:Antigen-Antibody Complex; D007074:Immunoglobulin G; D012709:Serum Albumin; D007073:Immunoglobulin E; D003165:Complement System Proteins