Growth of diseased human muscle in combined cultures with normal mouse embryonic spinal cord.
Normal and diseased human muscle cells have been grown in combined cultures with 12-14 day embryonic mouse spinal cord explants. Nerve endings on myotubes were found by light and scanning electron microscopy, and motor end-plates were identified by a histochemical reaction for acetylcholinesterase. Contracting myotubes, never observed in cultures of human muscle alone, were found in a culture from normal muscle. Histochemical studies demonstrated the presence of strongly and weakly reacting myotubes for both ATPase pH 9.4 and NADH-TR, but could not be related to the development of fibre types. No differences in morphology or histochemical reactions were found between normal and diseased muscle cells in these combined cultures.
D000110:Acetylcholinesterase / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D000818:Animals; D002648:Child; D002675:Child, Preschool; D003470:Culture Media; D046508:Culture Techniques; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D051379:Mice; D008870:Microtubules / Q000648:ultrastructure; D009132:Muscles / Q000201:enzymology / Q000503:physiopathology* / Q000648:ultrastructure; D009135:Muscular Diseases / Q000473:pathology / Q000503:physiopathology*; D009136:Muscular Dystrophies / Q000503:physiopathology; D009246:NADH Tetrazolium Reductase / Q000378:metabolism; D013116:Spinal Cord / Q000196:embryology*
D003470:Culture Media; D009246:NADH Tetrazolium Reductase; D000110:Acetylcholinesterase; D000251:Adenosine Triphosphatases