Protein synthesis in dextran sulfate-treated ascites tumor cells.
Treatment of ascites tumor cells with dextran sulfate resulted in a marked inhibition of the incorporation of [14C]valine into protein in the presence of a high Na+ medium. Amino acid incorporation was restored after i.p. injection of these cells into mice or by exposure of the cells to ascites fluid in vitro. In a medium high in K+ and low in Na+, [14C]valine incorporation into protein took place in dextran treated cells. Rotenone inhibited the reaction, which could be restored by addition of both inorganic phosphate and either glucose or glucose 6-phosphate. Quercetin, an inhibitor of the Na+-K+-ATPase, markedly depressed the incorporation of [14C]valine into protein in intact sdviyrd tumor cells in a high Na+ medium. There was little or no inhibition of protein synthesis in dextran sulfate treated cells when tested in a high K+-low Na+ medium. These experiments suggest a relationship between protein synthesis and the operation of the membranous Na+-K+-ATPase.
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000188:drug therapy*; D002462:Cell Membrane / Q000201:enzymology / Q000378:metabolism; D002463:Cell Membrane Permeability / Q000187:drug effects; D003911:Dextrans / Q000494:pharmacology*; D005947:Glucose / Q000494:pharmacology; D006019:Glycolysis / Q000187:drug effects; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D010101:Oxygen Consumption / Q000187:drug effects; D011188:Potassium / Q000378:metabolism; D011794:Quercetin / Q000494:pharmacology; D012402:Rotenone / Q000494:pharmacology; D012964:Sodium / Q000378:metabolism; D014633:Valine / Q000378:metabolism
D003911:Dextrans; D009363:Neoplasm Proteins; D012402:Rotenone; D011794:Quercetin; D012964:Sodium; D000251:Adenosine Triphosphatases; D014633:Valine; D005947:Glucose; D011188:Potassium