[Biosynthesis of macromolecules of the intercellular matrix in aorta organ culture. Influence of the age of the animal].
Rabbit aorta organ cultures were used as a model for the study of the modifications of the rate of biosynthesis of intercellular matrix macromolecules with age. Aortas of new born (100 g), young (400 g) and adult (2 kg) rabbits were maintained in culture for several weeks. 14C-lysine is incorporated in all the macromolecular fractions of the aortas, even in polymeric elastin. The change with age of the distribution of proteins of the aorta-explants in successive extracts obtained by a "chemical dissection" procedure and the decrease of their specific radioactivity wtih age enabled us to characterise biochemically the "aging program" of the arterial wall.
D000367:Age Factors; D000818:Animals; D001011:Aorta / Q000254:growth & development / Q000378:metabolism*; D003094:Collagen / Q000096:biosynthesis*; D003238:Connective Tissue / Q000378:metabolism; D004549:Elastin / Q000096:biosynthesis*; D006023:Glycoproteins / Q000096:biosynthesis*; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D008239:Lysine / Q000378:metabolism; D009924:Organ Culture Techniques; D011509:Proteoglycans / Q000096:biosynthesis*; D011817:Rabbits
D006023:Glycoproteins; D006025:Glycosaminoglycans; D011509:Proteoglycans; D003094:Collagen; D004549:Elastin; D008239:Lysine
Biosynthèse des macromolécules de la matrice intercellulaire en culture d'organe d'aorta. Influence de l'âge de l'animal