Biosynthesis of glycosaminoglycans by mammalian corneal epithelium and fibroblasts in vitro. I. Isolation and fractionation-differences of GAG from the two cell types.
Labeled glycosaminoglycans (GAG) were isolated and fractionated from cultured rabbit corneal epithelium, rabbit or bovine corneal fibroblasts and rabbit bone fibroblasts that had been previously incubated with either Na235SO4 or D-glucosamine-6-3H precursors. Thus it was demonstrated that the epithelium produces GAG. The fractionations indicated that the epithelial cells predominantly synthesize GAG with a lower degree of sulfation than those produced by corneal fibroblasts. The GAG-fractionation-patterns of the corneal epithelium and bone fibroblasts were similar.
D000818:Animals; D001842:Bone and Bones / Q000378:metabolism; D002417:Cattle; D005591:Chemical Fractionation; D002807:Chondroitin / Q000096:biosynthesis; D003315:Cornea / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D005944:Glucosamine / Q000378:metabolism; D006025:Glycosaminoglycans / Q000096:biosynthesis* / Q000302:isolation & purification; D006820:Hyaluronic Acid / Q000096:biosynthesis; D007632:Keratan Sulfate / Q000096:biosynthesis; D011817:Rabbits; D013431:Sulfates / Q000378:metabolism; D013997:Time Factors
D006025:Glycosaminoglycans; D013431:Sulfates; D006820:Hyaluronic Acid; D002807:Chondroitin; D007632:Keratan Sulfate; D005944:Glucosamine