[The functional coupling between MM isozyme of creatine phosphokinase (EC 2.7.3.2.) and MgATPase of myofibrils and (Na, K)ATPase of plasma membrane in heart cells].
The functional role of particulate MM isozyme of creatine phosphokinase (CPK) bound to heart myofibrils has been studied. It has been shown that in the presence of heart myofibrils and MgATP creatine phosphate can be used to rephosphorylate ADP formed in the MgATPase reaction. The rate of creatine phosphate splitting is determined by the kinetic properties of myofibrillar MgATPase and by the kinetic parameters of myofibrillar CPK. It has been found that a purified heart plasma membrane preparation contains high CPK activity. CPK isozyme bound to plasma membrane of heart cells is identical to MM isozyme of CPK and is able to rephosphorylate effectively ADP, formed in the (Na K)ATPase reaction. The rate of creatine phosphate splitting in these coupled reactions is sensitive to ouabain and is determined by the kinetic parameters both of the (Na, K)ATPase and plasma membrane CPK. The results obtained indicate the important role of myofibrillar and plasma membrane CPK in the intracellular energy transport processes.
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D003402:Creatine Kinase / Q000502:physiology*; D004735:Energy Transfer; D007527:Isoenzymes / Q000502:physiology*; D007700:Kinetics; D008274:Magnesium; D009206:Myocardium / Q000166:cytology / Q000201:enzymology*; D010085:Oxidative Phosphorylation; D011188:Potassium; D011485:Protein Binding; D051381:Rats; D012964:Sodium
D007527:Isoenzymes; D012964:Sodium; D003402:Creatine Kinase; D000251:Adenosine Triphosphatases; D008274:Magnesium; D011188:Potassium
Funktsional'noe soprazhenie MM-izofermenta kreatinfosfokinazy s Mg-ATPazoĭ miofibrill i Na, K-ATPazoĭ plazmaticheskoi membrany sepdechnykh kletok