[Comparative study of microbial methods for the detection of genetic toxicity].
It is important to have a rapid and accurate method to detect the toxic action of drugs and chemical compounds used by man. A comparative study with two microbial systems was carried out: one using Salmonella typhimurium and the other using Bacillus subtilis. The 1-8-dihydroxyantraquinone was the tested drug and the N-methyl-N'-nitro-N-nitrosoguanidine and the 2-aminofluorene were used as control substances. These compounds were used as such or after previous transformation by a microsomal system. The results obtained showed that both systems: the S. typhimurium and the B. subtilis work in a similar way, but the latter allows a more direct action of drug on the genetic material. The effect of the solvents: ethanol and dimethylsulfoxide was analysed, because they affected the transformation and reversion processes that were carried out.
D000880:Anthraquinones / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000235:genetics*; D001431:Bacteriological Techniques; D004269:DNA, Bacterial / Q000378:metabolism; D005449:Fluorenes / Q000494:pharmacology*; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology*; D009152:Mutagenicity Tests / Q000379:methods*; D012486:Salmonella typhimurium / Q000235:genetics*; D014170:Transformation, Genetic
D000880:Anthraquinones; D004269:DNA, Bacterial; D005449:Fluorenes; D008769:Methylnitronitrosoguanidine; C012177:2-aminofluorene
Estudio comparativo de métodos microbianos para la detección de tóxicos genéticos.