Antigen in immune complex nephritis. V. Recovery and identification by gel precipitation.
A procedure is described for detection of antibodies and antigens in 'immune complex' nephritis. Kidney tissue is minced, washed and sealed in a well of an agarose or agar plate. Separation of immune complexes is achieved by electrophoresis at 56 degrees. Afterwards, antibody is readily detected in reaction with a proper anti-IgG serum. Antigen is identified by its corresponding antibody, the reaction with which is, in some instances, strengthened by counterimmunoelectrophoresis.
D000328:Adult; D000818:Animals; D000906:Antibodies / Q000032:analysis; D000941:Antigens / Q000032:analysis*; D003165:Complement System Proteins / Q000032:analysis; D004247:DNA / Q000276:immunology; D005260:Female; D006801:Humans; D007105:Immune Complex Diseases; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000032:analysis; D007668:Kidney / Q000276:immunology; D008722:Methods; D009393:Nephritis / Q000276:immunology*; D011817:Rabbits
D000906:Antibodies; D000941:Antigens; D007074:Immunoglobulin G; D003165:Complement System Proteins; D004247:DNA