On the structure of heparitin sulfates. Analyses of the products formed from heparitin sulfates by two heparitinases and a heparinase from Flavobacterium heparinum.
The analyses of the products formed from heparitin sulfates by the action of two heparitinases and a heparinase from Flavorbacterium heparinum is reported. Heparitin sulfates A and B are degraded by heparitinase I yielding two disaccharides, one of them composed of N-acetylucosamine and an unsaturated uronic, joined by alpha(1 lead to 4) linkage, and the other, with the same composition but with an O-sulfate at the hexosamine moiety. A third disaccharide is also formed from heparitin sulfate B, by the action of the same enzyme, composed of glucosamine N-sulfate and an unsaturated uronic acid joined probably by alpha(1 lead to 4) linkage. Besides these three disaccharides, heparitin sulfate B yields, by the action of heparitinase I, an oligosaccharide (with an average molecular weight of 6000) which is completely degraded by the heparitinase II yielding a disaccharide composed of glucosamine 2,6-disulfate and unsaturated uronic acid. All the disaccharides are further degraded by alpha-glycuronidase from Flavobacterium heparinum yielding the respective monosaccharides. Based on these and other analyses the possible structures of the heparitin sulfates are proposed.
D000117:Acetylglucosamine / Q000032:analysis; D001780:Blood Coagulation Tests; D002413:Cations, Divalent; D002414:Cations, Monovalent; D004187:Disaccharides / Q000032:analysis; D005417:Flavobacterium / Q000201:enzymology*; D006025:Glycosaminoglycans; D006497:Heparan Sulfate; D006595:Hexosamines / Q000032:analysis; D007700:Kinetics; D008970:Molecular Weight; D009904:Optical Rotation; D011133:Polysaccharide-Lyases / Q000378:metabolism*; D013464:Sulfuric Acids / Q000032:analysis; D014574:Uronic Acids / Q000032:analysis
D000117:Acetylglucosamine; D002413:Cations, Divalent; D002414:Cations, Monovalent; D004187:Disaccharides; D006025:Glycosaminoglycans; D006497:Heparan Sulfate; D006595:Hexosamines; D011133:Polysaccharide-Lyases; D013464:Sulfuric Acids; D014574:Uronic Acids