Dissociation kinetics of tetra-S-carbamidomethylated plasmin-modified human somatotropin.
The dissociation of tetra-S-carbamidomethylated, plasmin-modified human somatotropin at 25 +/- 2 degrees C has been found to be a slow, exergonic equilibrium reaction; an equilibrium constant of 1.77 mol/L and a standard free energy of dissociation of -340 cal/mol have been calculated. The major products of the dissociation have been shown to consist of a monomeric form of the 51-residue carboxyl-terminal fragment and a trimeric form of the 134-residue amino-terminal fragment. The carboxyl-terminal fragment appears to be a random coil, while the amino-terminal fragment retains a considerable degree of secondary and tertiary structures.
D000595:Amino Acid Sequence; D002942:Circular Dichroism; D005341:Fibrinolysin; D013006:Growth Hormone; D006801:Humans; D007460:Iodoacetamide; D007461:Iodoacetates; D011487:Protein Conformation
D007461:Iodoacetates; D013006:Growth Hormone; D005341:Fibrinolysin; D007460:Iodoacetamide