[Synthesis of ribosomal RNA in the lymphocytes with 21-chromosome trisomy].
Normal and 21-trisomic lymphocytes isolated from whole blood were cultivated with phytohemagglutinin (PHA) for 21 hours and labeled with 3H-uridine. Polysomes were then extracted, using magnesium precipitation. The samples of polysome rRNA were disc-electrophoresed, and the specific activity of the 28S-RNA was determined. In spite of the decreased RNA-synthetic response of the 21-trisomic lymphocytes (determined earlier) the specific activity of the rRNA in the aberrant cells failed to differ from the normal. A decrease in the number of the PHA-responsive cells in the 21-trisomic lymphoid population is supposed.
D002904:Chromosomes, Human, 21-22 and Y; D004314:Down Syndrome / Q000097:blood / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000032:analysis / Q000096:biosynthesis*; D012270:Ribosomes / Q000378:metabolism; D014314:Trisomy
D012335:RNA, Ribosomal
Sintez ribosomal'noĭ RNK v limfotsitakh s trisomieĭ po 21-ĭ khromosome.