Further studies on gel filtration of urinary substances.
Urine specimens from healthy children were concentrated and filtered through Sephadex G-100 and G-25. The eluate was recovered in fractions of 5 ml each. Total hexoses, galactose, total proteins and total uronic acids were determined on each fraction. Total hexoses and galactose were eluted in 6 distinct peaks. The uronic acid containing GAG were eluted in 4 small peaks on G-25 and 1 peak on G-100. This peak is eluted always with high amounts of galactose containing glycoproteins.
D002675:Child, Preschool; D002850:Chromatography, Gel; D005690:Galactose / Q000652:urine; D006020:Glycopeptides / Q000652:urine*; D006025:Glycosaminoglycans / Q000652:urine*; D006601:Hexoses / Q000652:urine*; D006801:Humans; D008297:Male; D008970:Molecular Weight; D011507:Proteinuria; D014574:Uronic Acids / Q000652:urine
D006020:Glycopeptides; D006025:Glycosaminoglycans; D006601:Hexoses; D014574:Uronic Acids; D005690:Galactose