Effects of the epoxide hydrase inhibitor, 1,1,1-trichloropropane-2,3-oxide on the genetic activity of aflatoxin B1 metabolites in in vitro activation test systems.
The epoxide hydrase inhibitor 1,1,1-trichloroprophane-2,3-oxide (TCPO) was genetically active to cells of S. cerevisiae and conidia of N. crassa. This genetic activity could be eliminated or reduced to near spontaneous levels in the presence of the S-9 fraction of hamster liver homogenate. The addition of TCPO to an in vitro activation system containing aflatoxin B1 resulted in an increase in the genetic activity of aflatoxin B1, and this increase was dependent on the dose of TCPO. These results are discussed in relation to the possible metabolism of the promutagen aflatoxin B1.
D000348:Aflatoxins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D000818:Animals; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D004851:Epoxide Hydrolases / Q000037:antagonists & inhibitors*; D004852:Epoxy Compounds / Q000494:pharmacology*; D004988:Ethers, Cyclic / Q000494:pharmacology*; D005821:Genetic Techniques; D006843:Hydrocarbons, Chlorinated / Q000494:pharmacology*; D009153:Mutagens / Q000378:metabolism*; D009492:Neurospora crassa; D012441:Saccharomyces cerevisiae
D000348:Aflatoxins; D004852:Epoxy Compounds; D004988:Ethers, Cyclic; D006843:Hydrocarbons, Chlorinated; D009153:Mutagens; D004851:Epoxide Hydrolases