A method using fibrin-fixed Blue Dextran for determining the plasmin and plasmin inhibitor activities in human plasma.
The fibrinolytic activity of plasmin was determined by incubating with fibrin-fixed Blue Dextran as a substrate, the Blue Dextran released being proportional to the plasmin activity. The applicability of this method for rapid and accurate evaluation of fibrinolytic activity was demonstrated by dose-response curves with purified plasmin, plasmin generated by urokinase in human plasma and euglobulin. The method can also be used to determined plasmin inhibitors in plasma.
D004396:Coloring Agents; D003224:Congo Red / Q000378:metabolism; D003911:Dextrans; D004305:Dose-Response Relationship, Drug; D005337:Fibrin / Q000378:metabolism*; D005341:Fibrinolysin / Q000032:analysis* / Q000037:antagonists & inhibitors; D006146:Guanidines; D006801:Humans; D008751:Methylene Blue / Q000378:metabolism; D012712:Serum Globulins / Q000378:metabolism; D014568:Urokinase-Type Plasminogen Activator / Q000378:metabolism
D004396:Coloring Agents; D003911:Dextrans; D006146:Guanidines; D012712:Serum Globulins; D003224:Congo Red; D005337:Fibrin; C017080:blue dextran; D005341:Fibrinolysin; D014568:Urokinase-Type Plasminogen Activator; D008751:Methylene Blue