Mitogen-stimulated glutaraldehyde-fixed spleen cells: ability to stimulate in the mixed lymphocyte reaction and generate effector cells in cell-mediated lympholysis.
Mouse spleen cells treated with glutaralde lose their stimulating ability in the MLR. If the spleen cells are first converted to a blastogenic state by lipopolysaccharide and subsequently fixed with glutaraldehyde, their stimulating capacity is maintained.
D000447:Aldehydes / Q000494:pharmacology*; D000818:Animals; D003601:Cytotoxicity Tests, Immunologic; D004926:Escherichia coli / Q000276:immunology; D005260:Female; D005976:Glutaral / Q000494:pharmacology*; D007111:Immunity, Cellular; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D011135:Polysaccharides, Bacterial / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology*
D000447:Aldehydes; D011135:Polysaccharides, Bacterial; D005976:Glutaral