title: Initiation of adenovirus DNA replication does not occur via a hairpin mechanism.
abstract: Models have been proposed suggesting that initiation of adenovirus DNA replication might occur via a hairpin mechanism. A consequence of the models is a covalent linkage of progeny and parental DNA in newly completed molecules. Analysis of mature molecules from KB cells infected with adenovirus type 5 indicates that, if a hairpin mechanism is involved, the length of the hairpin must be shorter than 50 basepairs long. Recent nucleotide sequence analysis of the termini of adenovirus type 5 DNA (P.H. Steenbergh et al. (1977) Nucl. Acids Res. 4, 4371-4389) has shown that a hairpin of this size does not exist and that therefore a hairpin mechanism is unlikely.
mesh_terms: D000260:Adenoviruses, Human / Q000378:metabolism*; D001973:Bromodeoxyuridine / Q000378:metabolism; D004261:DNA Replication; D005467:Floxuridine / Q000378:metabolism; D008954:Models, Biological; D013936:Thymidine / Q000378:metabolism; D014779:Virus Replication
chemical_list: D005467:Floxuridine; D001973:Bromodeoxyuridine; D013936:Thymidine