title: Effects of streptozotocin added in vitro on insulin biosynthesis and release in isolated rat islets.
abstract: The effect of different concentrations of streptozotocin was tested in vitro on isolated rat islets. The following parameters of islet-cell activity were measured: 1) incorporation of 3H-leucine into proinsulin and into total islet protein; 2) IRI release into the medium, and 3) islet proinsulin content. It appears from the preliminary results shown here that both insulin release and biosynthesis were decreased by streptozotocin in a way which was dose- and time-dependent.
mesh_terms: D000818:Animals; D066298:In Vitro Techniques; D007328:Insulin / Q000096:biosynthesis / Q000378:metabolism*; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D007930:Leucine / Q000378:metabolism; D008297:Male; D011384:Proinsulin / Q000096:biosynthesis; D051381:Rats; D013311:Streptozocin / Q000494:pharmacology*
chemical_list: D007328:Insulin; D013311:Streptozocin; D011384:Proinsulin; D007930:Leucine