title: Adenosine diphosphate binding to sodium-plus-potassium ion-dependent adenosine triphosphatase. The role of lipid in the nucleotide-potassium ion interplay.
abstract: Delipidated dogfish rectal-gland Na++K+-ATPase (Na++K+-dependent adenosine triphosphatase), almost devoid of hydrolytic activity, is able to bind about 2nmol of ADP/mg of protein. The "affinity" of delipidated enzyme for ADP is not affected by K+ in concentrations that greatly decrease the "affinity" of native Na++K+-ATPase. The K+-sensitivity of the ADP binding is in part restored by relipidation with dioleoyl phosphatidylcholine.
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism*; D000251:Adenosine Triphosphatases / Q000378:metabolism*; D050356:Lipid Metabolism; D010713:Phosphatidylcholines / Q000378:metabolism; D011188:Potassium; D011485:Protein Binding; D012964:Sodium
chemical_list: D010713:Phosphatidylcholines; D000244:Adenosine Diphosphate; D012964:Sodium; D000251:Adenosine Triphosphatases; D011188:Potassium