[Cytochemical observations of hemocytes in decapod brachyuran crustacea (author's transl)].
Hemocytes from two species of crabs, Eriocheir sinensis and Carcinus maenas, have been submitted to different cytochemical reactions in order to bring out the nature of granules' content, either by specific coloration or be enzymatic digestion. Light as well as electron microscopy has been used. The granules appeared to be made essentially of basic proteins, rich in arginin and disulfide bridges but poor in tyrosin. These proteins are linked with nonglycogenic carbohydrates. Glycogen deposits of varied size are restricted to the cytoplasm in all the different cell-types but have never been detected inside the granules themselves. Lipids are poorly represented. The bulk of the granule material is made of neutral mucopolysaccharides although minute amounts of acid mucosubstances could be found in some instances.
D000818:Animals; D001120:Arginine / Q000032:analysis; D003386:Brachyura; D002241:Carbohydrates / Q000032:analysis; D003594:Cytoplasmic Granules / Q000032:analysis; D004220:Disulfides / Q000032:analysis; D006003:Glycogen / Q000032:analysis; D006025:Glycosaminoglycans / Q000032:analysis; D006458:Hemolymph / Q000032:analysis / Q000166:cytology*; D008055:Lipids / Q000032:analysis; D011506:Proteins / Q000032:analysis; D014443:Tyrosine / Q000032:analysis
D002241:Carbohydrates; D004220:Disulfides; D006025:Glycosaminoglycans; D008055:Lipids; D011506:Proteins; D014443:Tyrosine; D006003:Glycogen; D001120:Arginine
Etude cytochimique des hémocytes des crustacés décapodes brachyoures