Isolation on discontinuous gradients of bovine albumin of a subpopulation of human lymphocytes exhibiting precursor characteristics.
Purified subpopulations of lymphocytes were obtained from discontinuous bovine serum albumin (BSA) gradients and by rosetting sheep erythrocytes (E) or EAC1-3. Cells from layers 1 to 3 of the BSA gradient which failed to rosette with E were highly reactive with EAC1-3 and with anti-human IgM. Demonstrating a high spontaneous rate of DNA synthesis and primitive morphology, these cells responded to pokeweed mitogen, concanavalin A and alloantigens, but not to phytohemagglutinin or soluble antigens. Constituting from 0.5% to 3.0% of all lymphocytes from each of the tissues studied, these lymphocytes appear to represent an earlier stage in human lymphocyte development distinct from both T and B cells.
D000941:Antigens; D002448:Cell Adhesion; D002454:Cell Differentiation; D002469:Cell Separation; D003165:Complement System Proteins / Q000378:metabolism; D003601:Cytotoxicity Tests, Immunologic; D004912:Erythrocytes / Q000276:immunology; D006801:Humans; D007104:Immune Adherence Reaction; D007111:Immunity, Cellular; D037102:Lectins; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000648:ultrastructure; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D013499:Surface Properties
D000941:Antigens; D037102:Lectins; D011947:Receptors, Antigen, B-Cell; D003165:Complement System Proteins