title: [Stabilization of Na+, K+-adenosine triphosphatase by dimethyl sulfoxide under inactivation by urea].
abstract: Hydrophobic agents, e.g. methanol, ethanol, isopropanol, acetone and dioxane were shown to induce irreversible inactivation of Na+, K+-adenosine triphosphatase beginning with their concentrations of 20 to 35%, whereas dimethyl sulphoxide exerted similar effect only at concentration of 50% and higher. Urea also irreversibly inactivated Na+, K+-adenosine triphosphatase, beginning with a concentration of about 20%. It was found that, dimethyl sulphoxide contrary to the other hydrophobic agents studied, protected Na+, K+-adenosine triphosphatase against the inactivating (denaturing) action of urea. The highest stabilizing effect of dimethyl sulphoxide was displayed at concentrations from 20 to 30%.
mesh_terms: D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004355:Drug Stability; D011188:Potassium / Q000378:metabolism; D011489:Protein Denaturation; D012964:Sodium / Q000378:metabolism; D014508:Urea / Q000494:pharmacology*
chemical_list: D014508:Urea; D012964:Sodium; D000251:Adenosine Triphosphatases; D011188:Potassium; D004121:Dimethyl Sulfoxide
vernacular_title: Stabilizatsiia Na+, K+-adenozintrifosfatazy dimetilsul'foksidom pri inaktivatsii mochevinoĭ.