[Radioimmunoassay without extraction of L-triiodothyronine in human serum].
A radio-immunoassay of L-triiodothyronin is described. Blinding of T3 to TBG is prevented by salicylate (1%--w/v). The rabbit antiserum obtained by injection of a complexe T3-bovine serum albumin is diluted between 1/10000 and 1/20000. Bound and free hormone are separated by polyethylene glycol (17%--w/v). Serum T3-concentration in normal subjects averaged 132 +/- 29 ng/dl (SD). In hyperthyroid and hypothyroid patients, these values were respectively 351 +/- 118 ng/dl and 68 +/- 21 ng/dl.
D006801:Humans; D006980:Hyperthyroidism / Q000097:blood; D007037:Hypothyroidism / Q000097:blood; D011863:Radioimmunoassay / Q000379:methods; D014284:Triiodothyronine / Q000097:blood*
D014284:Triiodothyronine
Dosage radioimmunologique sans extraction de la L-triidothyronine dans le sérum humain