A simple and rapid method for isolation of reduced carbohydrate fragments from the linkage region of cartilage keratan sulphate.
The method described is a simple and rapid procedure for isolation in high yield of carbohydrate fragments containing terminal galactosaminitol derived from the polysaccharide-protein linkage region of cartilage keratan sulphate. It is based on the observation that reducing sugars bind tightly to Dowex-1 resin (hydroxide form), whereas reduced analogues (sugar alcohols) do not [H. Yamaguchi, S. Inamura & K. Makino (1976) J. Biochem. (Tokyo) 79, 299-303].
D000818:Animals; D002241:Carbohydrates / Q000302:isolation & purification*; D002356:Cartilage / Q000032:analysis*; D002417:Cattle; D006025:Glycosaminoglycans / Q000032:analysis*; D007475:Ion Exchange Resins; D007632:Keratan Sulfate / Q000032:analysis*; D008722:Methods
D002241:Carbohydrates; D006025:Glycosaminoglycans; D007475:Ion Exchange Resins; D007632:Keratan Sulfate