title: Bovine periodontal ligament. An invesitation of the collagen, glycosaminoglycan and insoluble glycoprotein components at different stages of tissue development.
abstract: Periodontal ligaments from unerupted, partially erupted and mature teeth were extracted with 0.15 M NaCl. The major reducible collagen cross-link in each insoluble fraction was dehydrodihydroxylysinonorleucine; the dehydroydroxylysinonorleucine contents were smaller. There was no significant difference in the quantities of these cross-links relative to collagen contents in the three speciments, but one of the precursors, hydroxyallysine, markedly decreased in the older tissue. The amino acid compositions of the trypsin-resistant insoluble fractions were generally characteristic of collagen. Analyses of separated glycopeptides revealed the presence of insoluble non-collagenous glycoproteins and collagen hexoses. The latter were lower in the mature ligament. Hyaluronic acid progressively decreased relative to chondroitin sulphate on eruption and maturation. A hyaluronidase-resistant glycosaminoglycan, probably dermatan sulphate, occurred in the NaCl-insoluble fraction of the mature ligament and in appreciable amounts in all NaCl extracts.
mesh_terms: D000818:Animals; D002417:Cattle; D002809:Chondroitin Sulfates / Q000032:analysis; D003094:Collagen / Q000032:analysis*; D003871:Dermatan Sulfate / Q000032:analysis; D005688:Galactosamine / Q000032:analysis; D005690:Galactose / Q000032:analysis; D005944:Glucosamine / Q000032:analysis; D005947:Glucose / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis*; D006025:Glycosaminoglycans / Q000032:analysis*; D006820:Hyaluronic Acid / Q000032:analysis; D006901:Hydroxylysine / Q000032:analysis; D008358:Mannose / Q000032:analysis; D010513:Periodontal Ligament / Q000032:analysis* / Q000033:anatomy & histology / Q000254:growth & development; D014574:Uronic Acids / Q000032:analysis
chemical_list: D006023:Glycoproteins; D006025:Glycosaminoglycans; D014574:Uronic Acids; D003871:Dermatan Sulfate; D006901:Hydroxylysine; D005688:Galactosamine; D006820:Hyaluronic Acid; D002809:Chondroitin Sulfates; D003094:Collagen; D005947:Glucose; D005944:Glucosamine; D008358:Mannose; D005690:Galactose