Inhibition of osteogenesis by bone mass in diffusion chamber cultures.
The effect of increased bone mass on osteogenesis was studied using the diffusion chamber culture of mouse distal radius as an experimental model. Two distal thirds of a mouse's radii were cultured in separate diffusion chambers in a recipient mouse; one chamber contained only a distal radius, but the other contained a distal radius plus two proximal radii and two ulnae. The DNA synthesis of osteogenetic cells was significantly inhibited in cultures with excess bone mass. The mean inhibition in labelling indices of epiphyseal plate chondrocytes was 27% and of periosteal cells 40%. The nature of this inhibition seems to be specific.
D000818:Animals; D001842:Bone and Bones / Q000166:cytology; D002478:Cells, Cultured; D003239:Connective Tissue Cells; D004247:DNA / Q000096:biosynthesis; D006131:Growth Inhibitors / Q000494:pharmacology*; D051379:Mice; D010012:Osteogenesis
D006131:Growth Inhibitors; D004247:DNA