title: 16Beta-hydroxylation of dehydroepiandrosterone sulphate by homogenates of human foetal liver.
abstract: The chemical syntheses of the 3-sulphates of 16 alpha-acetoxy-, 16 alpha-hydroxy- and 16 beta-hydroxy-dehydroepiandrosterone as their triethylammonium salts are described preparatory to studies of the metabolism of [7 alpha-3 H]dehydroepiandrosterone sulphate by homogenates of human foetal liver. Five main radioactive products of the incubation were separated by partition chromatography on a Celite column. Two were identified  as 16 alpha-and 16 beta-hydroxydehydroepiandrosterone 3-sulphates by crystallization to constant specific radioactivity before and after solvolysis. The yields of the conversion to the two epimers were 24.4% (16 alpha) and 1.8% (16 beta).
mesh_terms: D015114:Androstenediol; D002855:Chromatography, Thin Layer; D003687:Dehydroepiandrosterone / Q000031:analogs & derivatives / Q000138:chemical synthesis / Q000378:metabolism*; D005260:Female; D006801:Humans; D006900:Hydroxylation; D008099:Liver / Q000196:embryology* / Q000378:metabolism; D008297:Male
chemical_list: D003687:Dehydroepiandrosterone; D015114:Androstenediol