Stimulating capacity of human lymphoid cell subpopulations in mixed lymphocyte cultures.
The content and yield or B cells, T cells and monocytes were investigated in cell fractions separated from human peripheral blood by various techniques. These cell fractions, which were highly enriched for either B cells, T cells or monocytes, were tested for their capacity to stimulate allogenic peripheral blood mononuclear cells in mixed lymphocyte cultures (MLC). B cells were found to be strongly stimulatory. Monocytes stimulated strongly at low stimulator/responder cell ratios, while less stimulation was observed using larger amounts of cells. T cells were demonstrated to stimulate weakly, if at all.
D001402:B-Lymphocytes / Q000276:immunology; D002458:Cell Fractionation; D002469:Cell Separation; D006801:Humans; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000276:immunology*; D009000:Monocytes / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology