[Changes in ATPase activity of dog kidney nuclear membrane under the effect of polyenic antibiotics].
Effect of amphotericin B and nistatin on ATPase activity of dog kidney nuclear membranes is studied in vivo and in vitro. Long-term intravenous injections of the antibiotics do not change the ATPase activity of kidney nuclear membranes. However, short-term injections of polyenic antibiotics have some effect on ATPase activity on nuclear membranes: amphotericin B, considerably activates the enzyme . In vitro incubation of isolated dog kidney nuclei with amphotericin and nistatin at concentrations of 1 and 10 mcg/ml does not affect the activity of nuclear membrane ATPase, while increased concentrations of polyenic antibiotics, (up to 200 mcg/ml) results in a slight inhibition of the enzyme activity. The role of the data obtained for solving molecular basis of the toxic effect of polyenic antibiotics.
D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000666:Amphotericin B / Q000494:pharmacology* / Q000633:toxicity; D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology*; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation; D007668:Kidney / Q000201:enzymology*; D008566:Membranes / Q000201:enzymology; D009761:Nystatin / Q000494:pharmacology* / Q000633:toxicity
D009761:Nystatin; D000666:Amphotericin B; D000251:Adenosine Triphosphatases
Izmenenie ATPaznoĭ aktivnosti iadernykh membran pochek sobak pod vliianiem polienovykh antibiotikov.