Chondrocyte metabolism as affected by vitamin A.
Chondrocytes from 13-day-old embryonic chick sterna were cultured for 6 days in the presence of vitamin A (10 I.U./ml). Vitamin A treated chondrocytes became flattened and stellate within the first 24 hours of culture. After 6 days of culture, the treated cells contained 59% less DNA than the untreated controls. Sulfated glycosaminoglycan synthesis was inhibited 84%, and a greater percentage of GAG was secreted into the medium (90%) than in control cultures (78%). Vitamin A inhibited cell proliferation, and to varying degrees, RNA and protein synthesis, and these effects are dose dependent.
D000818:Animals; D002356:Cartilage / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D002809:Chondroitin Sulfates / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis; D003864:Depression, Chemical; D006025:Glycosaminoglycans / Q000096:biosynthesis / Q000378:metabolism*; D006497:Heparan Sulfate / Q000378:metabolism; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis; D014801:Vitamin A / Q000494:pharmacology*
D002809:Chondroitin Sulfates; D004247:DNA; D006025:Glycosaminoglycans; D006497:Heparan Sulfate; D012313:RNA; D014801:Vitamin A