title: A mathematical model for the influence of anionic effectors on the phosphofructokinase from rat erythrocytes.
abstract: The influence of the positive effectors AMP, sulphate, glucose 1,6-bisphosphate and the negative effector 2,3-bisphosphoglycerate on rat erythrocyte phosphofructokinase has been investigated. The kinetic data have been fitted to the Monod-Wyman-Changeux model as well as to a model based on a closed association-dissociation equilibrium. The application of the fitting procedure yeilds for both models a good corresponding between theoretical and experimental data and equal results with respect to the action of the effectors on the enzyme. The corresponding dissociation constants for the binding of the positive effectors to the active state are: AMP 35 micronM, sulphate 0.43 mM and glucose 1,6-bisphosphate 15 micronM. 2,3-Bisphosphoglycerate as in inhibitor stabilizes the inactive state (dissociation constant: 1.4 mM). A preliminary discrimination between the Monod-Wyman-Changeux model and the association-dissociation model has been attempted.
mesh_terms: D000249:Adenosine Monophosphate / Q000494:pharmacology; D000818:Animals; D004163:Diphosphoglyceric Acids / Q000494:pharmacology; D004912:Erythrocytes / Q000201:enzymology*; D005958:Glucosephosphates / Q000494:pharmacology; D006598:Hexosediphosphates / Q000494:pharmacology; D007700:Kinetics; D008433:Mathematics; D008954:Models, Biological; D010732:Phosphofructokinase-1 / Q000097:blood*; D051381:Rats; D013431:Sulfates / Q000494:pharmacology
chemical_list: D004163:Diphosphoglyceric Acids; D005958:Glucosephosphates; D006598:Hexosediphosphates; D013431:Sulfates; D000249:Adenosine Monophosphate; D010732:Phosphofructokinase-1