[Proteins of erythrocytic membrane. II. Solubilization of proteins in the membrane of human erythrocytes by chlorpromazine (author's transl)].
At the concentration above 2 mM, chlorpromazine induces partial solubilization of human erythrocytic membrane proteins. High molecular weight components such as spectrin are not extracted. Glycoproteins are preferentially released: at least five glycoprotein components are separated by acrylamide gel electrophoresis after specific staining. Other non identified proteins of the ghost external surface are also solubilized. At high concentration total solubilization of ghosts is obtained. Material extracted by chlorpromazine may be used for further protein purification.
D001798:Blood Proteins / Q000302:isolation & purification*; D002462:Cell Membrane / Q000032:analysis; D002746:Chlorpromazine; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000032:analysis*; D006023:Glycoproteins / Q000302:isolation & purification; D006801:Humans; D008970:Molecular Weight; D012967:Sodium Dodecyl Sulfate; D012995:Solubility
D001798:Blood Proteins; D006023:Glycoproteins; D012967:Sodium Dodecyl Sulfate; D002746:Chlorpromazine
Protéines de la membrane érythrocytaire ii. Solubilisation des protéines de la membrane d'érythrocytes humains par la chlorpromazine