[The immunobiology of M-proteins of Streptococcus pyogenes. I. Isolation and purification of M-proteins from group A streptococci (Streptococcus pyogenes) type 1].
Streptococci of group A, type 1, are lysed by use of phage associated lysin, which is characterized as a N-Acetyl-D-glucosaminidase. This enzyme has no proteolytic activity. Type specific protein antigens are isolated and purified by ion exchange chromatography on CM-cellulose and DEAE-cellulose of the crude extract, concentrated by precipitation with ammonium sulfate. The immunization tests are carried out on monkeys and rabbits.
D000818:Animals; D000942:Antigens, Bacterial / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000302:isolation & purification*; D000882:Haplorhini; D007114:Immunization; D011817:Rabbits; D013297:Streptococcus pyogenes / Q000276:immunology
D000942:Antigens, Bacterial; D001426:Bacterial Proteins
Untersuchungen zur Immunbiologie von M-Proteinen des Streptococcus pyogenes