[Technique for isolation and purification of metaphase chromosomes of KB cells].
Metaphase chromosomes were isolated from KB cells according to Salzman and Mendelsohn's method. Whole-mount electronic microscopic preparations of chromosomes in situ, after isolation and after purification showed that the morphological patterns of this material are preserved in spite of physical and chemical treatments (pH 3).
D002458:Cell Fractionation / Q000379:methods*; D002460:Cell Line; D002875:Chromosomes / Q000648:ultrastructure; D008854:Microscopy, Electron; D008938:Mitosis
Technique d'isolement et de purification de chromosomes métaphasiques de cellules KB