[Administration of immobilized firefly luciferase for quantitative estimation of ATP and enzymes synthesizing and destroying ATP].
A possibility of using immobilized Luciola mingrelica luciferase for quantitative test of ATP and ATP synthetizing and degrading enzymes activities is demonstrated. A kinetic scheme is given, and experimental conditions for using L. mingrelica luciferin-luciferase system for kinetic enzyme analysis are determined.
D000251:Adenosine Triphosphatases / Q000032:analysis; D000255:Adenosine Triphosphate / Q000032:analysis*; D000818:Animals; D001517:Coleoptera; D004800:Enzymes, Immobilized / Q000378:metabolism; D007700:Kinetics; D008156:Luciferases / Q000378:metabolism; D008433:Mathematics
D004800:Enzymes, Immobilized; D000255:Adenosine Triphosphate; D008156:Luciferases; D000251:Adenosine Triphosphatases
Primenenie immobilizovannoĭ liutsiferazy svetliakov dlia kolichestvennogo opredeleniia ATP i fermentov, sinteziruiushchikh i razlagaiushchikh ATP.