Separation of subfragment-1 of H-meromyosin into two equimolar fractions with and without formation of the reactive enzyme-phosphate-ADP complex.
H-Meromyosin (HMM) was digested with insoluble papain [EC 3.4.22.2].  Neither the size of the initial burst of Pi liberation (0.5 mole/mole of myosin head) nor the Mg2+-ATPase [EC 3.6.1.3] activity of HMM in the steady state was affected by this treatment.  Acto-S-1 was obtained by mixing F-actin with HMM digested with insoluble papain (HMM-S-1).  The size of the initial burst of Pi liberation of acto-S-1 was 0.35 mole/mole of S-l at an ATP concentration of 0.5 mole/mole of S-1, and 0.5 mole/moleof S-1 at ATP concentrations above 1 mole/mole of S-1...
D000199:Actins / Q000378:metabolism; D000205:Actomyosin / Q000378:metabolism; D000244:Adenosine Diphosphate; D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D015879:Myosin Subfragments; D009218:Myosins / Q000378:metabolism; D010206:Papain; D010710:Phosphates; D011485:Protein Binding; D011770:Pyruvate Kinase / Q000378:metabolism; D011817:Rabbits
D000199:Actins; D015879:Myosin Subfragments; D010710:Phosphates; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D000205:Actomyosin; D011770:Pyruvate Kinase; D010206:Papain; D000251:Adenosine Triphosphatases; D009218:Myosins