Estimation of peptidoglycan antibodies by an immunoperoxidase technique.
An immunoperoxidase technique developed for the detection and measurement of anti-peptidoglycan antibodies is described. The technique is based on the Mancini single radial immunodiffusion test with the addition of a reaction step with peroxidase-labelled anti-immunoglobulins. The dark-brown colour produced by the enzyme and a H2O2-diaminobenzidine solution increased the sensitivity considerably. The test was found to be highly specific and valuable both for the screening of total amounts of anti-peptidoglycan antibodies in sera as well as in studies of antibody specificity.
D015100:3,3'-Diaminobenzidine; D000906:Antibodies / Q000032:analysis*; D000918:Antibody Specificity; D006735:Horseradish Peroxidase; D005779:Immunodiffusion; D007074:Immunoglobulin G; D008722:Methods; D010457:Peptidoglycan / Q000276:immunology*
D000906:Antibodies; D007074:Immunoglobulin G; D010457:Peptidoglycan; D015100:3,3'-Diaminobenzidine; D006735:Horseradish Peroxidase