A membrane permeability test for the detection of cell surface antigens.
A convenient microtest is described which utilizes antibody-mediated release of [14C]nicotinamide ([14C]NA) from target cells for the detection of cell surface antigens. This test is considerably more sensitive and faster than the widely used 51Cr release test because most of the [14C]NA is rapidly released from the target cells in the initial phase of membrane permeability changes induced by activated complement, as distinct from the colloid osmotic phase of complement-mediated cytolysis.
D000818:Animals; D000937:Antigen-Antibody Reactions; D000941:Antigens; D000951:Antigens, Neoplasm; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology*; D002463:Cell Membrane Permeability; D003165:Complement System Proteins; D003601:Cytotoxicity Tests, Immunologic; D005347:Fibroblasts / Q000276:immunology / Q000378:metabolism; D008722:Methods; D051379:Mice; D009536:Niacinamide / Q000276:immunology / Q000378:metabolism; D012513:Sarcoma, Experimental / Q000276:immunology
D000941:Antigens; D000951:Antigens, Neoplasm; D009536:Niacinamide; D003165:Complement System Proteins