Determination of urinary dehydroepiandrosterone using 3beta-hydroxysteroid dehydrogenase.
A method for the determination of urinary dehydroepiandrosterone (DHA) using 3beta-hydroxysteroid dehydrogenase is described. The method includes extraction with ethylacetate, solvolysis, and color development using 3beta-hydroxysteroid dehydrogenase. A study of comparison with gas-liquid chromatography method and recovery test was made, and results were sufficient for routine tests.
D002849:Chromatography, Gas / Q000379:methods; D003124:Colorimetry / Q000379:methods; D003687:Dehydroepiandrosterone / Q000652:urine*; D006801:Humans; D006913:Hydroxysteroid Dehydrogenases; D013053:Spectrophotometry; D013056:Spectrophotometry, Ultraviolet
D003687:Dehydroepiandrosterone; D006913:Hydroxysteroid Dehydrogenases