title: Biosynthesis of glycosaminoglycans by mammalian corneal epithelium and fibroblasts in vitro. I. Isolation and fractionation-differences of GAG from the two cell types.
abstract: Labeled glycosaminoglycans (GAG) were isolated and fractionated from cultured rabbit corneal epithelium, rabbit or bovine corneal fibroblasts and rabbit bone fibroblasts that had been previously incubated with either Na235SO4 or D-glucosamine-6-3H precursors. Thus it was demonstrated that the epithelium produces GAG. The fractionations indicated that the epithelial cells predominantly synthesize GAG with a lower degree of sulfation than those produced by corneal fibroblasts. The GAG-fractionation-patterns of the corneal epithelium and bone fibroblasts were similar.
mesh_terms: D000818:Animals; D001842:Bone and Bones / Q000378:metabolism; D002417:Cattle; D005591:Chemical Fractionation; D002807:Chondroitin / Q000096:biosynthesis; D003315:Cornea / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism; D005944:Glucosamine / Q000378:metabolism; D006025:Glycosaminoglycans / Q000096:biosynthesis* / Q000302:isolation & purification; D006820:Hyaluronic Acid / Q000096:biosynthesis; D007632:Keratan Sulfate / Q000096:biosynthesis; D011817:Rabbits; D013431:Sulfates / Q000378:metabolism; D013997:Time Factors
chemical_list: D006025:Glycosaminoglycans; D013431:Sulfates; D006820:Hyaluronic Acid; D002807:Chondroitin; D007632:Keratan Sulfate; D005944:Glucosamine