title: Isolation of putrescine-requiring mutants of Neurospora crassa.
abstract: Two auxotrophs of Neurospora crassa have been isolated that give a positive growth response to putrescine, spermidine or spermine. One of the mutants is deficient in ornithine decarboxylase activity and has been designated put-I. Both mutants map on linkage group VR, fail to complement and are infertile when crossed to one another, indicating that they are probably alleles. A putrescine auxotroph is incapable of suppressing a pro-4 mutant. The isolation of the mutants confirms that putrescine is an essential factor the normal growth of the organism, and is synthesized via a single pathway in Neurospora.
mesh_terms: D003433:Crosses, Genetic; D005816:Genetic Complementation Test; D008040:Genetic Linkage; D009154:Mutation; D009491:Neurospora / Q000378:metabolism*; D009492:Neurospora crassa / Q000235:genetics / Q000378:metabolism*; D009955:Ornithine Decarboxylase / Q000378:metabolism; D011700:Putrescine / Q000378:metabolism*; D013095:Spermidine / Q000378:metabolism; D013096:Spermine / Q000378:metabolism
chemical_list: D013096:Spermine; D009955:Ornithine Decarboxylase; D013095:Spermidine; D011700:Putrescine