Determination of the antigen binding capacity of antisera to collagen by gel filtration.
Significant differences in the antigen binding capacity of different antisera to collagen of identical haemagglutination titres were demonstrated by using gel filtration for the isolation of collagen-anticollagen immune-complexes. A partially degraded collagen was used as the antigen. The influence of antigen concentration on antigen binding capacity was investigated with 2 antisera of different avidity. The distribution of antibody-bound antigen among IgM- and IgG- peaks indicates the participation of both immunoglobulin classes in the formation of collagen-anticollagen immune-complexes.
D000818:Animals; D000937:Antigen-Antibody Reactions; D001665:Binding Sites; D002850:Chromatography, Gel; D003094:Collagen; D003488:Cyanogen Bromide; D007106:Immune Sera; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011817:Rabbits / Q000276:immunology; D012995:Solubility
D007106:Immune Sera; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D003094:Collagen; D003488:Cyanogen Bromide