Antibody-dependent cell-mediated cytotoxicity in humans. I. Characterization of the effector cell.
Mononuclear cells from human peripheral blood are able to destroy alloantibody-coated target cells of a human lymphoblastoid line. The effector cell is a nonadherent, non-phagocytic, medium sized lymphoid cell. The cell is not detectable in early human fetuses. Its activity is destroyed by x-ray treatment in vitro but is not inhibited by anti-immunoglobulin sera. Its efficiency in antibody-dependent cell-mediated cytotoxicity is rapidly lost in culture and this loss is prevented by stimulation with allogeneic cells.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000302:isolation & purification; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D000941:Antigens; D001803:Blood Transfusion; D003571:Cytochalasin B / Q000494:pharmacology; D003601:Cytotoxicity Tests, Immunologic; D005333:Fetus / Q000276:immunology; D006680:HLA Antigens / Q000008:administration & dosage; D006801:Humans; D007106:Immune Sera / Q000302:isolation & purification; D007111:Immunity, Cellular; D007128:Immunoglobulin Fragments / Q000032:analysis; D007518:Isoantibodies / Q000032:analysis; D007840:Latex / Q000032:analysis; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000276:immunology* / Q000528:radiation effects; D008863:Microspheres; D008870:Microtubules / Q000187:drug effects; D011817:Rabbits / Q000276:immunology; D011830:Radiation Effects; D014965:X-Rays
D000888:Antibodies, Anti-Idiotypic; D000941:Antigens; D006680:HLA Antigens; D007106:Immune Sera; D007128:Immunoglobulin Fragments; D007518:Isoantibodies; D007840:Latex; D003571:Cytochalasin B