title: Effect of substituted pyridazinone herbicides and of difunone (EMD-IT 5914) on carotenoid biosynthesis in green algae.
abstract: The carotenoid biosynthesis of the green alga Ankistrodesmus braunii is blocked if these cells are cultured in presence of sublethal doses of pyridazinone herbicides (San 9789, San 6706, BASF 44521) or of the herbicide difunone (EMD-IT 5914). The amount of colored carotenoids normally found in these algae is reduced drastically and the precursors phytoene and phytofluene are accumulated. Furthermore a decrease in the chlorophyll level occurs in the treated cells, but there is a stronger loss of chlorophyll a, resulting in a lowering of the chlorophyll a/b ratio with time. Concerning the activity of substituted pyridazinones leading to inhibition of carotenoid biosynthesis this effect can be related to the chemical structure of these compounds: a trifluormethyl substitution of the phenyl ring and a mono- or dimethyl substitution of the amine (San 9789, San 6706) or a methoxy group instead of the substituted amine (BASF 44521) are required both for this effect. Other pyridazinone derivatives with either a trifluoromethyl substitition of the phenyl ring (San 9774) or a dimethyl subsittution of the amine (San 9785) or a methoxy group (BASF 13761) are without any effect on the pigment pattern of these algae.
mesh_terms: D002338:Carotenoids / Q000096:biosynthesis*; D000460:Chlorophyta / Q000187:drug effects / Q000378:metabolism*; D005663:Furans / Q000494:pharmacology*; D006540:Herbicides / Q000494:pharmacology*; D011724:Pyridazines / Q000494:pharmacology*; D013053:Spectrophotometry; D013056:Spectrophotometry, Ultraviolet; D013329:Structure-Activity Relationship
chemical_list: D005663:Furans; D006540:Herbicides; D011724:Pyridazines; D002338:Carotenoids