title: Energy requirements for biosynthesis of DNA in Escherichia coli. Role of membrane-bound energy-transducing ATPase (coupling factor).
abstract: A mutant of Escherichia coli missing energy-transducing ATPase and known to be defective in a variety of membrane functions from earlier studies (Yamamoto, T. H., Mével-Ninio, M. and Valentine, R. C. (1973) Biochim. Biophys. Acta 314, 267-275; Thipayathasana, P. and Valentine, R. C. (1974) Biochim. Biophys. Acta 347, 464-468; Mével-Ninio, M. and Yamamoto, T. (1974) Biochim. Biophys. Acta 357, 63-66) has been found to be blocked for anaerobic DNA synthesis. The rate of anaerobic DNA synthesis in the mutant, measured as radioactive adenine incorporation into the alkali-resistant fraction of whole cells, is about 1/6 the rate of DNA synthesis in the wild type culture under similar conditions. Addition of NO-3- or O-2 restores DNA biosynthesis in the mutant. The entry of radioactive adenine is not appreciably affected in the mutant by anaerobiosis. It is concluded that coupling factor plays a role in some step(s) of DNA biosynthesis.
mesh_terms: D000225:Adenine / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000332:Aerobiosis; D000693:Anaerobiosis; D001693:Biological Transport, Active; D002455:Cell Division; D002462:Cell Membrane / Q000378:metabolism*; D002465:Cell Movement; D002874:Chromosome Mapping; D004269:DNA, Bacterial / Q000096:biosynthesis*; D004926:Escherichia coli / Q000378:metabolism*; D009154:Mutation; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D013997:Time Factors; D014161:Transduction, Genetic
chemical_list: D004269:DNA, Bacterial; D009247:NADH, NADPH Oxidoreductases; D000251:Adenosine Triphosphatases; D000225:Adenine