Macrophage-T cell interactions in the Con A induction of human suppressive T cells.
Macrophage-T interactions are required for the Con A-induced generation of human Ts capable of inhibiting PHA-induced blastogenesis among autologous PBMC. Con A treatment of adherent cell-depleted PBMC, or PBMC recovered after a 7-day incubation in FCS, failed to generate Ts. Addition of adherent cells to either of these populations restored Con A inducible Ts. Discontinuous density gradient fractionation of adherent cells demonstrated that the required accessory cell was a low density macrophage bearing the human equivalent of murine Ia.
D000818:Animals; D002448:Cell Adhesion; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D003208:Concanavalin A / Q000494:pharmacology*; D006649:Histocompatibility Antigens; D006801:Humans; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology*
D006649:Histocompatibility Antigens; D003208:Concanavalin A