An inhibitor of DNA synthesis in erythrocyte-conditioned medium and its separation from haemoglobin.
Erythrocyte-conditioned medium and erythrocyte lysate have been prepared from rat red blood cells. Molecular fractions of the conditioned medium and the lysate are used to test for the presence of erythrocyte chalone in short-term tissue cultures of mouse foetal liver cells. An inhibitor of erythroblast DNA synthesis has been found in the erythrocyte conditioned medium. The inhibitor is not haemoglobin, and it is not found in erythrocyte lysate soluble components.
D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D004247:DNA / Q000096:biosynthesis*; D004912:Erythrocytes / Q000378:metabolism*; D005333:Fetus / Q000033:anatomy & histology; D006131:Growth Inhibitors / Q000097:blood* / Q000502:physiology; D006454:Hemoglobins / Q000032:analysis; D008099:Liver / Q000166:cytology; D051379:Mice; D051381:Rats
D003470:Culture Media; D006131:Growth Inhibitors; D006454:Hemoglobins; D004247:DNA