Isolation and characterization of myosin from cloned rat glioma and mouse neuroblastoma cells.
Myosin has been isolated from the clonal lines of murine neuroblastoma and rat glioma cells. Partial characterization of the two cellular myosins indicates that both possess the following properties: (1) the same elution position as rabbit skeletal muscle myosin by Sepharose 4B chromatography; (2) the presence of heavy (molecular weight about 200,000) and light subunit polypeptides by sodium dodecyl sulfate polyacrylamide gel electrophoresis; (3) EDTA and Ca2+ activated but Mg2+-inhibited ATPase activity in 0.6 M KCl; and (4) binding to rabbit skeletal muscle F-actin which is inhibited by Mg2+-ATP. For both mouse neuroblastoma and rat glioma cells, approximately 0.5-1.5% of the total cell protein is present as myosin. Cellular myosin appears to be indistinguishable in quantity and biochemical properties regardless of whether it is isolated from monolayer or suspension neuroblastoma cells.
D000199:Actins / Q000302:isolation & purification; D000251:Adenosine Triphosphatases; D000818:Animals; D002118:Calcium; D002384:Catalysis; D055598:Chemical Phenomena; D002621:Chemistry; D002999:Clone Cells; D004492:Edetic Acid; D004591:Electrophoresis, Polyacrylamide Gel; D005910:Glioma / Q000032:analysis*; D008274:Magnesium; D051379:Mice; D009132:Muscles / Q000032:analysis; D009218:Myosins / Q000302:isolation & purification*; D009447:Neuroblastoma / Q000032:analysis*; D011817:Rabbits; D051381:Rats; D012967:Sodium Dodecyl Sulfate
D000199:Actins; D012967:Sodium Dodecyl Sulfate; D004492:Edetic Acid; D000251:Adenosine Triphosphatases; D009218:Myosins; D008274:Magnesium; D002118:Calcium