Chemical characteristics of chalones isolated from bovine spleen.
Isolation of spleen chalones from the postmicrosomal supernatant is described. Two glycoprotein fractions homogeneous on polyacrylamide-gel electrophoresis were obtained. In the biological test on mice, the preparation of 38 000 mol. wt. inhibited mitosis in spleen cells, and the low-molecular fraction (2100 mol. wt.) in thymus cells, showing respectively the properties of chalones B and T.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D006023:Glycoproteins / Q000302:isolation & purification / Q000494:pharmacology; D006131:Growth Inhibitors / Q000302:isolation & purification / Q000494:pharmacology; D008198:Lymph Nodes / Q000187:drug effects; D051379:Mice; D008938:Mitosis / Q000187:drug effects; D008970:Molecular Weight; D011506:Proteins / Q000032:analysis; D013154:Spleen / Q000032:analysis*; D013950:Thymus Gland / Q000187:drug effects
D000596:Amino Acids; D002241:Carbohydrates; D006023:Glycoproteins; D006131:Growth Inhibitors; D011506:Proteins