title: Reaction mechanism of erythrocyte phosphofructokinase.
abstract: The reaction mechanism of erythrocyte phosphofructokinase (PFK) was investigated by the initial velocity and the product inhibition. Intersecting lines obtained with initial velocity studies are consistent with a sequential mechanism and the formation of ternary complex as an intermediate. The product inhibition studies support an ordered Bi Bi mechanism in which fructose 6 phosphate (F6P) is the first substrate binding and adenosine diphosphate (ADP) is dissociated from the enzyme before fructose-1,6-P2 (FDP).
mesh_terms: D000244:Adenosine Diphosphate / Q000494:pharmacology; D004912:Erythrocytes / Q000201:enzymology*; D005635:Fructosediphosphates / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D010732:Phosphofructokinase-1 / Q000097:blood*
chemical_list: D005635:Fructosediphosphates; D000244:Adenosine Diphosphate; D010732:Phosphofructokinase-1