Stimulation of fetal spleen cells with lipopolysaccharides.
LPS proved to be good B-cell mitogen for fetal pig spleen cells and induced proliferation as well as differentiation of virgin resting small lymphocytes within 48 hours of cultivation. The LPS-activated lymphocytes and blast-like cells were more intensely labeled with anti-Ig antibodies than control cells, most probably due to an increased density of surface Ig. A lower percentage of LPS-activated cells was found to be labeled with F(ab)2 fragment of anti-L than with anti-L antibodies having an intact Fc fragment. The latter finding suggests non-specific binding of antisera to Fc receptors which appear to be activated on LPS-stimulated lymphoid cells.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D001666:Binding Sites, Antibody; D005333:Fetus; D007140:Immunoglobulin Fab Fragments; D007141:Immunoglobulin Fc Fragments; D008070:Lipopolysaccharides / Q000276:immunology*; D008213:Lymphocyte Activation; D011947:Receptors, Antigen, B-Cell; D013154:Spleen / Q000166:cytology*; D013552:Swine
D007140:Immunoglobulin Fab Fragments; D007141:Immunoglobulin Fc Fragments; D008070:Lipopolysaccharides; D011947:Receptors, Antigen, B-Cell