Effect of delayed addition of 2-mercaptoethanol on the generation of mouse cytotoxic T lymphocytes in mixed leukocyte cultures.
The generation of mouse cytolytic T lymphocytes (CTL) in primary or secondary mixed leukocyte cultures (MLC) is greatly enhanced by the addition of 2-mercaptoethanol (2-ME) to the culture medium. This enhancement can be equally demonstrated with either reduced or oxidized 2-ME. Addition of 2-ME to MLC as late as 3 days after the initiation of the culture results in a peak CTL response which is nearly as high as the one in cultures containing 2-ME throughout the 4 day-incubation period. Quantitative analysis of the CTL response in MLC supplemented with 2-ME on day 3 shows a 50-fold increase in lytic activity within 24 h, suggesting that cell differentiation, in addition to proliferation may be affected by this agent.
D000818:Animals; D003601:Cytotoxicity Tests, Immunologic; D005260:Female; D007111:Immunity, Cellular / Q000187:drug effects*; D007959:Lymphocyte Culture Test, Mixed; D008623:Mercaptoethanol / Q000494:pharmacology*; D051379:Mice; D008815:Mice, Inbred Strains; D010084:Oxidation-Reduction; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D013997:Time Factors
D008623:Mercaptoethanol