Glycolysis and glycogen metabolism after inhibition of hexose monophosphate pathway in C6-GLIAL cells.
1. 6-Aminonicotinamide (0.01 mg/ml) leads to a strong accumulation of 6-PG in C6 glial cells after 24h. 2. The accumulated 6-PG is dephosphorylated to gluconate which easily permeates the cell membrane. Extracellular gluconate is formed at a rate of 12% of the total glucose consumption. 3. 6-PG as competitive inhibitor of the PG1 caused a reduction of the glycolytic flux of about 40%. 4. The reduced glycolytic flux lowers the ATP concentration under anaerobic conditions to 75% of the controls. 5. The glycogen content after 6-AN is increased by 50%, probably by the activation of the glycogen synthetase due to the higher Glc 6-P concentration. 6. The fibroblast-like morphology of the C6 cell line has typically changed under 6-aminonicotinamide.
D015120:6-Aminonicotinamide / Q000494:pharmacology*; D000818:Animals; D001921:Brain / Q000378:metabolism; D002478:Cells, Cultured; D005942:Gluconates / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism*; D006019:Glycolysis / Q000187:drug effects*; D006600:Hexosephosphates / Q000378:metabolism*; D009457:Neuroglia / Q000187:drug effects* / Q000378:metabolism; D009536:Niacinamide / Q000031:analogs & derivatives*; D051381:Rats
D005942:Gluconates; D006600:Hexosephosphates; D009536:Niacinamide; D015120:6-Aminonicotinamide; D006003:Glycogen