title: Native (Na-+ + K-+)-dependent adenosine triphosphatase has two trypsin-sensitive sites.
abstract: Sodium and potassium adenosine triphosphatase ((Na + K)-ATPase) consists of two polypeptides, a large molecular weight polypeptide (MW 84,000 to 102,000) and a sialoglycoprotein (MW 35,000 to 57,000). Trypsin treatment of this complex selectively cleaves the large polypeptide into two fragments with molecular weights of 62,000 and 43,000. Simultaneously with the appearance of these fragments, (Na + K)-APTase activity is destroyed. Trypsin treatment of phosphorylated enzyme shows that he 43,000 molecular weight fragment is phosphorylated. If (Na + K)-ATPase is digested with trypsin in the presence of ATP, a 90,000 molecular weight fragment is produced. Disappearance of the large polypeptide, and loss of ATPase activity parallel the production of this fragment. Addition of strophanthidin to this mixture significantly lowers the amount of the 90,000 molecular weight fragment produced. Experiments on (Na + K)-ATPase of the red cell membrane suggest that trypsin is cleaving (Na + K)-ATPase at the interior surface of the plasma membrane.
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002414:Cations, Monovalent / Q000494:pharmacology; D002462:Cell Membrane / Q000378:metabolism; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000201:enzymology; D006801:Humans; D007679:Kidney Medulla / Q000201:enzymology; D008274:Magnesium / Q000494:pharmacology; D008970:Molecular Weight; D010761:Phosphorus Radioisotopes; D011188:Potassium / Q000494:pharmacology; D011485:Protein Binding; D012964:Sodium / Q000494:pharmacology; D012967:Sodium Dodecyl Sulfate; D013327:Strophanthidin / Q000494:pharmacology; D013395:Sucrose / Q000494:pharmacology; D014357:Trypsin / Q000378:metabolism*
chemical_list: D002414:Cations, Monovalent; D010761:Phosphorus Radioisotopes; D012967:Sodium Dodecyl Sulfate; D013395:Sucrose; D013327:Strophanthidin; D000255:Adenosine Triphosphate; D012964:Sodium; D014357:Trypsin; D000251:Adenosine Triphosphatases; D008274:Magnesium; D011188:Potassium