title: Does phospholipase C stimulate thymide kinase activity of rat liver extracts prepared after partial hepatectomy.
abstract: Our purpose was to determine whether phospholipase C stimulated thymidine kinase activity of regenerating rat liver. We determined effects of phospholipase C upon TMP formation by rat liver extracts prepared at 0, 12, 24, 36 and 48 hr following partial hepatectomy. Data were obtained which supported these conclusions: (a) Commercial preparations of phospholipase C contained nucleoside phosphotransferase activity; (b) phospholipase C exerted no appreciable stimulatory influence upon thymidine kinase activity of regenerating rat liver; and (c), apparent stimulation of thymidine kinase was associated with linked activities of two enzymes, viz., liver extract-ATPase activity and nucleoside phosphotransferase activity.
mesh_terms: D000249:Adenosine Monophosphate / Q000494:pharmacology; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D005260:Female; D005459:Fluorides / Q000494:pharmacology; D006498:Hepatectomy; D008099:Liver / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D008110:Liver Extracts; D008115:Liver Regeneration / Q000187:drug effects*; D010740:Phospholipases / Q000494:pharmacology*; D010770:Phosphotransferases / Q000378:metabolism; D051381:Rats; D013268:Stimulation, Chemical; D013937:Thymidine Kinase / Q000096:biosynthesis / Q000378:metabolism*
chemical_list: D008110:Liver Extracts; D000249:Adenosine Monophosphate; D000255:Adenosine Triphosphate; D010770:Phosphotransferases; D013937:Thymidine Kinase; D010740:Phospholipases; D000251:Adenosine Triphosphatases; D005459:Fluorides