[Studies on the mechanism of activation of lymphocyte membrane ATP-ases by concanavalin A (author's transl)].
The ouabain-sensitive and ouabain-intensitive ATP-ases of intact lymphocytes, and of the lymphocyte microsomal fraction and a purified plasma membrane fraction, are all activated by concanavalin A. The Na+, K+, and Mg+-dependence of the plasma membrane ATP-ases were defined at different substrate concentrations. Enzyme activation was rather greater at lower K+ concentrations. From these studies it appears improbable, but not impossible, that concanavalin A exerts an allosteric effect on the ATP-ases.
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000494:Allosteric Regulation; D002462:Cell Membrane / Q000201:enzymology; D003208:Concanavalin A / Q000494:pharmacology*; D004789:Enzyme Activation; D006801:Humans; D008214:Lymphocytes / Q000201:enzymology*; D008861:Microsomes / Q000201:enzymology; D010042:Ouabain / Q000494:pharmacology
D003208:Concanavalin A; D010042:Ouabain; D000251:Adenosine Triphosphatases
Untersuchungen über den Mechanismus der Aktivierung der Lymphocytenmembran-ATPasen durch Concanavalin A