[Conditions and limits of serum LH radioimmunoassay in normal, hypophysectomized or castred rats].
Serum LH was measured by radioimmunoassay (NIAMD Kits) free and linked hormones were separated by double antibodies method. Influence of concentration on antibody-hormone complex is studied. Hypophysectomised rats serum does not modify results. The standard (rat LH-RP1) has the same action as serum LH. Rat serum LH contents are measured in normal or castred rats.
D000818:Animals; D000937:Antigen-Antibody Reactions; D002369:Castration; D011232:Chemical Precipitation; D002852:Chromatography, Ion Exchange; D005260:Female; D007016:Hypophysectomy; D007457:Iodine Radioisotopes; D007986:Luteinizing Hormone / Q000097:blood*; D011863:Radioimmunoassay; D051381:Rats
D007457:Iodine Radioisotopes; D007986:Luteinizing Hormone
Conditions et limites du dosage radioimmunologique de la lh sérique de rats normaux, hypophysectomisés ou castrés