Purification and characterization of an anti-inflammatory factor (AIF) from bovine serum.
An anti-inflammatory factor (AIF) was highly purified from normal bovine serum. The purified AIF was a polysaccharide which was formed from a low molecular substance Pro-AIF by macromolecularization. AIF showed potent inhibitory activities against carrageenin induced edema in rats and PMN-leucocyte chemotaxis. It showed no inhibition in cutaneous reaction with serotonin, bradykinin or a mediator releaser compound 48/80. No inhibition was also observed in thermally induced pain, reversed passive Arthus reaction and adjuvant polyarthritis in rats. With these results, the role of AIF in inflammation was discussed.
D000700:Analgesics; D000818:Animals; D001169:Arthritis, Experimental / Q000503:physiopathology; D001183:Arthus Reaction / Q000503:physiopathology; D002199:Capillary Permeability / Q000187:drug effects; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D005260:Female; D066298:In Vitro Techniques; D007249:Inflammation / Q000503:physiopathology; D008297:Male; D009504:Neutrophils / Q000187:drug effects; D011134:Polysaccharides / Q000097:blood*; D051381:Rats; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
D000700:Analgesics; D011134:Polysaccharides