[Detection of immunoglobulins in pharyngeal tonsillar tissue by direct immunofluorescence technique (author's transl)].
Using a direct immunofluorescent staining method a qualitative determination of different immunoglobulin fractions in the tissue of hypertropic pharyngeal tonsils of children and youth suffering from repeated infections was performed. It was found that the immunofluorescent staining of IgG was stronger than of IgA. A difference with regard to the frequency of appearance between IgG and IgA could not be seen. On the other hand IgM staining was less frequent and strong. Extracellular IgG was present in germinal centres of the secondary lymphoid follicles, intracellular one was found in the immunocompetent cells. Extra- and intracellular IgG was more often demonstrable in the spaces of the lymphoid tissue, between the surface epithelial cells or around the glands and the blood cappilaries. The results seen here are in correlation with the values of the earlier performed quantitative estimations of the different immunoglobulin classes.
D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D005110:Extracellular Space / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007136:Immunoglobulins / Q000032:analysis*; D014066:Palatine Tonsil / Q000276:immunology*
D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins
Nachweis der Immunglobuline im Gewebe pharyngealer Tonsillen mittels der direkten Immunfluoreszenzmethode.