Disparity of mixed lymphocyte reactivity to cultured cells of human T and B lymphoid lines.
Repeated attempts with difference assays and experimental conditions failed to detect significant peripheral blood lymphocyte reactivity in one-way mixed lymphocyte reactions to irradiated or mitomycin C-treated cells of the "leukemic" T lymphoid line RPMI 8402. In contrast, consistently high levels of peripheral blood lymphocyte reactivity were obtained with cells of six B lymphoid lines established from the same blood sample used to initiate this T lymphoid line. Although attempts to define the reason why these cultured T cells did not initiate a mixed lymphocyte reaction were not successful, evidence indicates that this inability may be an intrinsic characteristic common to three other T lymphoid lines (MOLT-4, CCRF-CEM, and CCRF-HSB-2), also established from patients with relapsed acute lymphoblastic leukemia.
D001402:B-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D006801:Humans; D007104:Immune Adherence Reaction; D007945:Leukemia, Lymphoid / Q000276:immunology*; D007959:Lymphocyte Culture Test, Mixed; D009439:Neuraminidase / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology*
D009439:Neuraminidase