HL-A LD (lymphocyte defined) typing: a rapid assay with primed lymphocytes.
When human lymphocytes are cultured for 9 to 14 days with stimulating cells of a family member differing by a single HL-A haplotype they become "primed" to recognize specific HL-A LD (mixed lymphocyte culture) antigens. These primed lymphocytes respond specifically and rapidly when "restimulated" with cells of a person that contain the same LD antigens as those of the priming haplotype. Specific HL-A LD antigens can be detected within 24 hours by this primed LD typing.
D000483:Alleles; D002455:Cell Division; D005260:Female; D006084:Graft Rejection; D006680:HLA Antigens; D006649:Histocompatibility Antigens; D006801:Humans; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed / Q000379:methods*; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D006680:HLA Antigens; D006649:Histocompatibility Antigens; D014316:Tritium; D013936:Thymidine