Single gene alteration of plasma and mitochondrial membrane function in Saccharomyces cerevisiae.
Some physiological properties of a multiple-drug-resistant mutant with a permeability barrier to chloramphenicol and its isogenic parental strain were compared. The ATPase specific activity of plasma and mitochondrial membranes isolated from the mutant strain was approximately 20% lower (P less than 0.001, Tables 1 and 2) than that of membranes isolated from the isogenic parental strain. Additional evidence of altered mitochondrial function was: (i) the enhanced growth of the parental strain was eliminted by the [rho-] state (Table 3); (ii) the mutant strain had a greater resistance to petite induction by ethidium bromide (Table 4); (iii) the mutant strain was unable to use a nonfermentable energy source for respiratory adaptation (Table 5). It is proposed that a single gene mutation has resulted in an alteration of some physiological properties of the plasma and mitochondrial membranes.
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D002462:Cell Membrane / Q000201:enzymology*; D002701:Chloramphenicol / Q000494:pharmacology; D004352:Drug Resistance, Microbial; D004996:Ethidium / Q000494:pharmacology; D005796:Genes; D008928:Mitochondria / Q000201:enzymology* / Q000648:ultrastructure; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000201:enzymology*; D012641:Selection, Genetic
D002701:Chloramphenicol; D000251:Adenosine Triphosphatases; D004996:Ethidium