title: Biosynthesis of [7-3H]16 alpha-hydroxy-dehydroepiandrosterone having high specific activity.
abstract: Biosynthesis of [7-3H]16alpha-hydroxy-dehydroepiandrosterone in high specific activity has been studied. [7-3H] dehydroepiandrosterone (13.9 C/mM) in trace quantity was oxidized by Streptomyces roseochromogenes (NRRLB-1233) for 5 min at 27 degrees C. The radioactive products were chromatographically separated, identified and their radiochemical purity established by isotopic dilution analysis. [7-3H]16alpha-hydroxy-dehydroepiandrosterone (2.5 x 10(7) dpm) was obtained by microbial hydroxylation of substrate (1.9 X 10(9) dpm). In some cases [7-3H])5-androstene-3beta, 16alpha, 17beta-triol in a small amount of radioactivity could be found at the prolonged reaction for 30 hr.
mesh_terms: D003687:Dehydroepiandrosterone / Q000031:analogs & derivatives* / Q000378:metabolism; D006900:Hydroxylation; D013302:Streptomyces / Q000378:metabolism*; D014316:Tritium
chemical_list: D014316:Tritium; C011277:16-hydroxydehydroepiandrosterone; D003687:Dehydroepiandrosterone