Action of cytochalasin A, a sulfhydryl-reactive agent, on sugar metabolism and membrane-bound adenosine triphosphatase of yeast.
Cytochalasin A at 10-20 mug/ml inhibits growth and sugar uptake by Saccharomyces strain 1016. The effects of cytochalasin A in intact cells were completely prevented when 1 mM cysteine or dithiothreitol was added along with cytochalasin A, but were not eliminated by thiols added after inhibition had occurred. Purified yeast hexokinase, glucose-6-P dehydrogenase, phosphofructokinase and aldolase were not sensitive to cytochalasin A (20 mug/ml). Glyceraldehyde-3-P dehydrogenase was strongly inhibited by cytochalasin A (5 mug/ml); activity was promptly restored by thiols. Anaerobic glycolysis was inhibited by cytochalasin A or by iodoacetate; unlike iodoacetate, cytochalasin A did not cause accumulation of sugar phosphates. In contrast, cytochalasin A, but not iodoacetate, inhibited isolated membrane-bound ATPases. Cytochalasin A is a sulfhydryl-reactive agent and has membrane-related effects (adenosine triphosphatase) which may well be the basis of its interference with energy-dependent uptake of solutes.
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D003545:Cysteine / Q000494:pharmacology; D003572:Cytochalasins / Q000494:pharmacology*; D004024:Dicyclohexylcarbodiimide / Q000494:pharmacology; D004229:Dithiothreitol / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism*; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D006019:Glycolysis / Q000187:drug effects; D007461:Iodoacetates / Q000494:pharmacology; D009840:Oligomycins / Q000494:pharmacology; D012440:Saccharomyces / Q000254:growth & development / Q000378:metabolism*; D013403:Sugar Phosphates / Q000378:metabolism
D003572:Cytochalasins; D007461:Iodoacetates; D009840:Oligomycins; D013403:Sugar Phosphates; D004024:Dicyclohexylcarbodiimide; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D000251:Adenosine Triphosphatases; D005947:Glucose; D003545:Cysteine; D004229:Dithiothreitol