Identification and turnover of glycosaminoglycans in rat kidneys.
The turnover of sulfate label in crude glycosaminoglycan fractions from rat kidney cortex, medulla, and papilla has been determined. Heparan sulfate, chondroitin sulfate, dermatan sulfate, and hyaluronate have been separated electrophoretically and their specific activities determined after injection of labeled sulfate or glucose. The half-lives of the sulfated glycosaminoglycans are within the ranges found for other organs and tissues, but hyaluornate has a somewhat faster turnover in the kidney than elsewhere.
D000818:Animals; D002250:Carbon Radioisotopes; D002807:Chondroitin / Q000378:metabolism; D003871:Dermatan Sulfate / Q000378:metabolism; D004586:Electrophoresis; D004588:Electrophoresis, Cellulose Acetate; D005947:Glucose / Q000378:metabolism; D006025:Glycosaminoglycans / Q000378:metabolism*; D006497:Heparan Sulfate / Q000378:metabolism; D006820:Hyaluronic Acid / Q000378:metabolism; D007668:Kidney / Q000378:metabolism*; D007672:Kidney Cortex / Q000378:metabolism; D007679:Kidney Medulla / Q000378:metabolism; D051381:Rats; D013455:Sulfur / Q000378:metabolism; D013462:Sulfur Radioisotopes; D013997:Time Factors
D002250:Carbon Radioisotopes; D002807:Chondroitin; D003871:Dermatan Sulfate; D005947:Glucose; D006025:Glycosaminoglycans; D006497:Heparan Sulfate; D006820:Hyaluronic Acid; D013455:Sulfur; D013462:Sulfur Radioisotopes