[Heterogeneity of ovine prolactin after labeling with iodine 125: value of labeling with lactoperoxidase].
The authors have shown an heterogeneity of the ovine prolactine molecule after labelling with iodine 125. As well with chloramine T as with lactoperoxydase, it appears three molecular species which react with the immune serum antiprolactine (I.S.). The first species is of high molecular weight and is probably constituted of aggregates. Their combination with the I.S. is non specific and give blanks of high value. The second species is a dimere and the third one is the monomere. The two last species react with the I.S. and can give competition curves when they are choosen as tracer. However, if one uses as tracer a product obtained by labelling with chloramine T, the competition appears for high concentrations of native hormone. As if the I.S. recognizes much more the labelled protein than the native one. But if one uses the same species but labelled with lactoperoxydase, the competition appears for concentrations lower than five nanogrammes. In the same time one can see that the specificity toward different I.S. is modified. The authors think that the labelling with lactoperoxydase better preserves the tertiary structure of the native protein than do the labelling with chloramine T.
D000818:Animals; D002700:Chloramines; D007118:Immunoassay / Q000379:methods*; D007457:Iodine Radioisotopes; D007553:Isotope Labeling; D007784:Lactoperoxidase; D008968:Molecular Conformation; D010544:Peroxidases; D011388:Prolactin / Q000032:analysis*; D011863:Radioimmunoassay; D012756:Sheep
D002700:Chloramines; D007457:Iodine Radioisotopes; D011388:Prolactin; D007784:Lactoperoxidase; D010544:Peroxidases
Hétérogénéité de la prolactine ovine après marquage à l'iode 125: intérêt du marguage à la lactoperoxydase