The use of viable frozen lymphocytes for studies in human tumor immunology.
Viable frozen lymphocytes displayed activity in blastogenesis assays that was indistinguishable from freshly prepared lymphoid cells. Similarly, cytotoxic activity of lymphocytes against melanoma target cells from melanoma patients was only slightly affected by the freezing procedure. Frozen  lymphocytes provided a highly reproducible source of cells in these assays. The use of viable frozen peripheral blood lymphoid cells for the retrospective analysis of a cancer patient's immune response is described.
D000328:Adult; D001793:Blood Preservation; D002469:Cell Separation; D002470:Cell Survival; D002478:Cells, Cultured; D003208:Concanavalin A; D003601:Cytotoxicity Tests, Immunologic; D005615:Freezing; D006649:Histocompatibility Antigens; D006801:Humans; D037102:Lectins; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D008545:Melanoma / Q000097:blood / Q000276:immunology*; D008937:Mitomycins; D012189:Retrospective Studies; D013299:Streptodornase and Streptokinase; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014373:Tuberculin
D006649:Histocompatibility Antigens; D037102:Lectins; D008937:Mitomycins; D014373:Tuberculin; D014316:Tritium; D003208:Concanavalin A; D013299:Streptodornase and Streptokinase; D013936:Thymidine