Sampling the fetoplacental circulation. II. Combined laparoscopy-fetoscopy in the pregnant ovine.
Induction of pneumoperitoneum, laparoscopy, and intrauterine endoscopy (fetoscopy) were successfully accompished in 23 pregnant sheep at 45 to 60 days' gestational age. The fetoscope, a conventional 2.7 mm. Hopkins straightforward wide-angle telescope (Stortz 27018A) encased in a prototype 3.1 mm. eccentric cannula, incorporates a 25 gauge needle channel. Multiple pregnancy was encountered in 11 animals. Forty-two separate fetoscopic trials were accomplished; a fetoplacental blood sample was obtained in 52 per cent of the trials. A mean interval of 19 +/- 9 days (+/- S.D., range 6 to 37 days) was allowed between fetoscopy trials. Fetal deaths occurred in nine animals (39 per cent). We believe that laparoscopy permits preselection of the proposed fetoscopy puncture site and will permit the operator to thereby avoid the placenta and/or uterine vessels.
D000818:Animals; D001800:Blood Specimen Collection; D005260:Female; D005312:Fetal Blood; D005332:Fetoscopy / Q000379:methods*; D010535:Laparoscopy / Q000379:methods*; D008954:Models, Biological; D011028:Pneumoperitoneum, Artificial; D011247:Pregnancy; D011270:Pregnancy, Animal; D011296:Prenatal Diagnosis / Q000379:methods*; D012756:Sheep