The effects of concanavalin A, cerulenin, hydroxyurea and tunicamycin on the incorporation of amino acids and glucosamine in Tetrahymena pyriformis.
1. Pulse labelling of conjugating Tetrahymena pyriformis with glycine and glucosamine gave results consistent with a requirement for de novo synthesis of a lipid-like component which is involved in the addition of sugar to cell glycoprotein. 2. Conjugation of complementary strains of Tetrahymena can be inhibited without gross morphologic and metabolic changes in the cells. The observed inhibition of conjugation is reversible. 3. Concanavalin A stopped conjugation and stimulated both glycine and glucosamine incorporation. 4. Tunicamycin inhibited conjugation but had little influence on total glucosamine incorporation. Amino acid incorporation was slightly enhanced. 5. Hydroxyurea blocked conjugation, inhibited the incorporation of glucosamine and seemed to induce a transient increase in glycine incorporation. 6. Cerulenin inhibited conjugation but had little effect on the incorporation of glycine and glucosamine into the cell.
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology*; D002569:Cerulenin / Q000494:pharmacology*; D003208:Concanavalin A / Q000494:pharmacology*; D005944:Glucosamine / Q000378:metabolism*; D006918:Hydroxyurea / Q000494:pharmacology*; D013769:Tetrahymena pyriformis / Q000378:metabolism*; D013997:Time Factors; D014415:Tunicamycin / Q000494:pharmacology*
D000596:Amino Acids; D000935:Antifungal Agents; D003208:Concanavalin A; D014415:Tunicamycin; D002569:Cerulenin; D005944:Glucosamine; D006918:Hydroxyurea