The presence of phosphate-binding protein in inner mitochondrial membrane.
Phosphate-binding protein(s) was found in the inner mitochondrial membrane of calf heart by Sephadex G-200 and G-25 gel filtration. The binding activity was inhibited by N-ethylmaleimide and competed by a large amount of cold phosphate. The amount of phosphate bound to the fraction was 29 nmoles per mg of protein. Affinity chromatography with phosphate-bound Sepharose 4B confirmed the presence of phosphate-binding protein(s) in the active fraction of mitochondrial membrane fractionated by gel filtration.
D002352:Carrier Proteins / Q000378:metabolism*; D002850:Chromatography, Gel; D008931:Mitochondria, Muscle / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism*
D002352:Carrier Proteins; D010710:Phosphates