Modification of erythrocyte physicochemical properties by millimolar concentrations of glutaraldehyde.
The effects of low levels of glutaraldehyde uptake (less than 120 mumol/10(10) cells) on the physicochemical properties of human red blood cells (RBC) were investigated. Salient effects include: by different measures of cell deformability, the extent of glutaraldehyde uptake required to decrease cellular deformability was shown to range from approximately 8 to 30 mumol/10(10) cells; osmotically stressed red cells exhibit complete hemolysis when the level of glutaraldehyde uptake is less than 28 mumol/10(10) cells and no hemolysis when uptake is less than 70 mumol/10(10) cells with the extent of hemolysis decreasing in an approximately linear manner with glutaraldehyde uptake between these limits; glutaraldehyde uptake of up to 58 mumol/10(10) cells does not change the cells' density, mean cell volume or ability to retain potassium.
D000447:Aldehydes / Q000494:pharmacology*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003432:Cross-Linking Reagents / Q000494:pharmacology; D004906:Erythrocyte Count / Q000187:drug effects; D004907:Erythrocyte Deformability / Q000187:drug effects; D004909:Erythrocyte Indices / Q000187:drug effects; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D005976:Glutaral / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D009996:Osmotic Fragility / Q000187:drug effects; D011188:Potassium / Q000097:blood
D000447:Aldehydes; D003432:Cross-Linking Reagents; D011188:Potassium; D005976:Glutaral