Properties of an antiserum against Streptomyces hydrogenans 20beta-hydroxysteroid dehydrogenase.
Antiserum against crystallized 20beta-hydroxysteroid dehydrogenase from Streptomyces hydrogenans was used for different immunodiffusion and immunoprecipitation tests to quantify the bacterial enzyme in cell-free supernatants of the microorganism. After immunoprecipitation and gel electrophoresis the molecular weight of the subunits of 20beta-hydroxysteroid dehydrogenase was calculated to be 27 300 +/- 700.
D000936:Antigen-Antibody Complex; D004591:Electrophoresis, Polyacrylamide Gel; D006913:Hydroxysteroid Dehydrogenases / Q000096:biosynthesis / Q000276:immunology*; D007106:Immune Sera; D005779:Immunodiffusion; D008970:Molecular Weight; D012967:Sodium Dodecyl Sulfate; D013302:Streptomyces / Q000201:enzymology*
D000936:Antigen-Antibody Complex; D007106:Immune Sera; D012967:Sodium Dodecyl Sulfate; D006913:Hydroxysteroid Dehydrogenases