title: In vitro growth of cytotoxic human lymphocytes. I. Growth of cells sensitized in vitro to alloantigens.
abstract: Human lymphocytes sensitized to allogeneic determinants in vitro were continuously cultured on medium prepared from phytohemagglutinin-(PHA-P) stimulated human mixed lymphocyte cultures. With such human-conditioned medium (HCM), human peripheral lymphoid cells could be grown in culture for over 90 days with a doulbing time of about 54 hr. Cytotoxic lymphocytes could be grown in culture for this time with no loss of cytotoxicity or change in cytotoxic specificity.
mesh_terms: D002478:Cells, Cultured; D003470:Culture Media; D003602:Cytotoxicity, Immunologic; D006801:Humans; D007519:Isoantigens; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000276:immunology*; D013997:Time Factors
chemical_list: D003470:Culture Media; D007519:Isoantigens