[Purification and characterization of epidermal G2- and G1-chalones].
Highly purified epidermal G1- and G2-chalones from rat skin inhibit the entering of epidermocytes to S and M phases of cell cycle respectively. Their biological activity is characterized by tissue-specificity and not by species-specificity. Both of them are tissue-specific glycoproteins as for their antigenic properties. Molecular weight of G1-chlone is 21 000, G2-chalone--34 000, isoelectric point (pH) 5.55 and 5.85 respectively. G2-chalone is the fastest as compared to G1-chalone in 5% acrylamide gel electrophoresis, pH 8.3. When injected in rabbits, G2-chalone produced monospecific antibodies which have no cross-reactivity with G1-chalone. The amino acid composition of both chalones and immunofluorescent localization of G2-chalone in epidermal tisues are given.
D000596:Amino Acids / Q000032:analysis; D002453:Cell Cycle / Q000187:drug effects; D055598:Chemical Phenomena; D002621:Chemistry; D006023:Glycoproteins / Q000302:isolation & purification; D006131:Growth Inhibitors / Q000276:immunology / Q000302:isolation & purification* / Q000494:pharmacology; D008970:Molecular Weight; D009928:Organ Specificity; D012867:Skin / Q000032:analysis*; D014018:Tissue Distribution
D000596:Amino Acids; D006023:Glycoproteins; D006131:Growth Inhibitors
Ochistka i kharakteristika epidermla'nykh G2-i G1-keĭlonov.