[A new method for the determination of tissue sensitivity to dental materials in cell cultures].
This paper discribes a method that enables a qualitative analysis of the biological effects of stomatological materials upon living cells cultivated in vitro to be made. This method allows to make photographic records, at regular intervals of time, of those cells which are originally in a state of suspension, then start to adhere to the bottom of the vessel used for purposes of cultivation, when they become flat and wider. This method takes of the capability of cytoplasmic membranes to respond to any kind of chemical or physical noxa. Quick-motion pictures taken at regular intervals of time using ciné film provide for a quantitative differentiation and determination of the ratio of dilated to nondilated cells, which can be analyzed statistically.
D001681:Biological Assay / Q000379:methods*; D002460:Cell Line; D003764:Dental Materials / Q000494:pharmacology*
D003764:Dental Materials
Neue Methode zur Bestimmung der Gewebsverträglichkeit stomatologischer Materialien an Zellkulturen.