title: Induction of the catalytic protein of (Na+ plus K+)-ATPase in the salt gland of the duck.
abstract: The (Na+ plus K+)-ATPase activities in salt gland homogenates increased 3- to 4-fold after saline treatment of ducks for 3 weeks. The ATPase was purified to a specific activity of 460 and 1015 mumol Pi/mg protein per h, respectively, in control and saline-treated ducks. The catalytic protein was identified on polyacrylamide electrophoresis gels by phosphorylating the enzyme with (32P)ATP. The molecular weight of the protein was estimated to be 98 000. The amount of catalytic unit increased commensurately with the enzyme activity after saline treatment. It is therefore concluded that the increased enzyme activity is due to a de novo enzyme synthesis and is not an activation effect. Phospholipid concentration in the salt gland tissue increased 1.7-fold after the saline treatment. Significant increases occurred in the percentage of the total phospholipids as phosphatidylserine and sphingomyelin. In the partially purified (Na+ plus K+)-ATPase preparation, the percentage composition of phosphatidylserine and phosphatidylethanolamine increased after saline treatment.
mesh_terms: D000251:Adenosine Triphosphatases / Q000096:biosynthesis* / Q000302:isolation & purification; D000818:Animals; D004372:Ducks; D004789:Enzyme Activation / Q000187:drug effects; D004790:Enzyme Induction / Q000187:drug effects; D008297:Male; D008970:Molecular Weight; D009929:Organ Size; D010743:Phospholipids / Q000378:metabolism; D011188:Potassium / Q000494:pharmacology; D014176:Protein Biosynthesis; D012491:Salt Gland / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D012964:Sodium / Q000378:metabolism / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology
chemical_list: D010743:Phospholipids; D012965:Sodium Chloride; D012964:Sodium; D000251:Adenosine Triphosphatases; D011188:Potassium