[Chromosomal structure of Pseudomonas testosteroni. II. Activity of the endogenous RNA-polymerase (author's transl)].
After careful lysis the nucleoid of Pseudomonas testosteroni can be isolated in three different forms with compact and unfolded DNA structures. The released nucleoids contain endogenous DNA-dependent RNA-polymerase activity using the chromosomal DNA as a template. RNA synthesis is proportional to duration of RNA-polymerase reaction and amount of DNA-protein-complexes. The sensitivity towards ionic strength and rifampicin indicates that a part of RNA-polymerase activity is tightly bound to the chromosomal DNA.
D002875:Chromosomes / Q000378:metabolism*; D004269:DNA, Bacterial / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D009994:Osmolar Concentration; D011549:Pseudomonas / Q000201:enzymology*; D012329:RNA, Bacterial / Q000096:biosynthesis; D012293:Rifampin / Q000494:pharmacology; D013696:Temperature
D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012321:DNA-Directed RNA Polymerases; D012293:Rifampin
Chromosomale Strukturen von Pseudomonas testosteroni. II. Aktivität der endogenen RNA-Polymerase