[Leukocyte migration inhibition test in skin chamber--an in-vivo method].
For the demonstration of a cell-mediated sensitisation, tuberculin allergics, chromate allergics, penicillin allergics, and controls were investigated. The method used was the measurement of leukocyte migration inhibition in a Teflon skin chamber with and without addition of antigen, which approaches to testing under in vivo conditions. In 10 of 11 tuberculin allergics, 6 of 7 chromate allergics, and 7 penicillin allergics, a clear inhibition of leucocyte migration after addition of antigen could be observed (maximum 16 h after addition of antigen). In 16 unsensitised controls no significant migration inhibition appeared. Possible advantages of this method over in vitro methods and intracutaneous tests are discussed.
D002464:Cell Migration Inhibition; D002840:Chromates / Q000009:adverse effects; D004342:Drug Hypersensitivity / Q000175:diagnosis*; D006801:Humans; D007962:Leukocytes; D010406:Penicillins / Q000009:adverse effects; D012882:Skin Tests; D012884:Skin Window Technique; D014373:Tuberculin / Q000009:adverse effects
D002840:Chromates; D010406:Penicillins; D014373:Tuberculin
Leukozyten-Migrations-Hemmtest in der Hautkammer--eine In-vivo-Methode