Purification of histamine receptor, (III) Characterization of receptor rich membrane fraction of small intestinal smooth muscle of the cat.
Intracellular localization of histamine receptors in small intestinal smooth muscle of the cat was studied by investigating distribution of marker enzymes in the receptor rich fraction. Distribution of membrane markers coincided with that of the radiochemically labeled receptor fraction. Membrane fraction was further purified and it was concluded that the histamine receptor rich fraction is mainly composed of cell membrane. The chemical composition of the fraction lent support to this conclusion.
D000251:Adenosine Triphosphatases / Q000032:analysis; D000818:Animals; D002415:Cats; D002462:Cell Membrane / Q000032:analysis / Q000201:enzymology; D005591:Chemical Fractionation; D004950:Esterases / Q000032:analysis; D066298:In Vitro Techniques; D007421:Intestine, Small / Q000032:analysis; D009130:Muscle, Smooth / Q000032:analysis* / Q000201:enzymology; D011968:Receptors, Histamine / Q000302:isolation & purification*
D011968:Receptors, Histamine; D004950:Esterases; D000251:Adenosine Triphosphatases