Fragmentation of porcine beta-lipotropic hormone with plasmin.
Porcine beta-lipotropic hormone was digested by plasmin and the fragments released were isolated and identified. Based on the structure and the yield of the plasmin fragments and also on the time-course of the digestion, the relative sensitivity of the theoretically susceptible bonds towards the action of plasmin was estimated. The Lys79-Asn80 bond was the most susceptible to plasmin, the Arg51-Trp52 and Arg60-Tyr61 bonds were split to a lesser extent than the Lys79-Asn80 bond, but much faster than the Lys46-Met47, Lys69-Ser70 and Lys84-Asn85 bonds of the beta-lipotropic homone structure. Further lysyl and arginyl bonds of the molecule were not attacked under our experimental conditions.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D004590:Electrophoresis, Paper; D005341:Fibrinolysin; D006801:Humans; D010446:Peptide Fragments / Q000032:analysis; D013552:Swine; D014357:Trypsin; D008083:beta-Lipotropin
D000596:Amino Acids; D010446:Peptide Fragments; D008083:beta-Lipotropin; D014357:Trypsin; D005341:Fibrinolysin