Cytochemical studies of rosette-forming cells.
Two types of rosette-forming cells were identified: rosette-forming cells with membrane-bound receptors for sheep erythrocytes (E-RFC) and rosette-forming cells with Fc receptors (EA-RFC). In both types of lymphocytes the activity of acid phosphatase, beta-glucuronidase, non-specific esterase and the content of glycogen were studied. A significant difference was established between E-RFC and EA-RFC lymphocytes as concerns acid phosphatase activity; corresponding mean values being 72.4 +/- 7.5 respectively 44.4 +/- 4.5 (p less than 0.01). As oppose to the acid phosphatase activity the glycogen was significantly higher in EA-RFC lymphocytes (mean 67.2 +/- 6.9), as compared with E-RFC (mean 41.6 +/- 9.2). It seems to us that estimations of acid phosphatase activity may be of value in differentiating 2 populations of lymphocytes.
D000135:Acid Phosphatase / Q000097:blood; D000328:Adult; D000818:Animals; D001665:Binding Sites; D004912:Erythrocytes / Q000276:immunology; D004950:Esterases / Q000097:blood; D005260:Female; D005966:Glucuronidase / Q000097:blood; D006003:Glycogen / Q000097:blood; D006801:Humans; D007104:Immune Adherence Reaction; D007141:Immunoglobulin Fc Fragments; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D011947:Receptors, Antigen, B-Cell; D012756:Sheep / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D007141:Immunoglobulin Fc Fragments; D011947:Receptors, Antigen, B-Cell; D006003:Glycogen; D004950:Esterases; D000135:Acid Phosphatase; D005966:Glucuronidase