The effect of anions on Mg-ATPase in red blood cells.
Anions exert an influence on the passive permeability of Na+ and K+ in erythrocytes. THE EFFECT ON Mg-ATPase activity has been studied in human erythrocytes. 40 mM bicarbonate increased the activity as compared to the effect of 40 mM chloride; 20 mM sulphate inhibited it. Salicylate acted first as an activator then as an inhibitor of Mg-ATPase; maximum activity was reached at 60 mM CONCENTRATION. Thiocyanate inhibited saponin-stimulated Mg-ATPase, Ki = 1.85 X 10(-2)M. The probable mechanisms of action of the above anions on Mg-ATPase and possible relation to passive permeability of Na+ and K+ ions are discussed.
D000251:Adenosine Triphosphatases / Q000097:blood*; D001639:Bicarbonates / Q000494:pharmacology; D002712:Chlorides / Q000494:pharmacology*; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D008274:Magnesium / Q000097:blood; D011188:Potassium / Q000097:blood; D012459:Salicylates / Q000494:pharmacology*; D012503:Saponins / Q000494:pharmacology; D012964:Sodium / Q000097:blood; D013431:Sulfates / Q000494:pharmacology*; D013861:Thiocyanates / Q000494:pharmacology*
D001639:Bicarbonates; D002712:Chlorides; D012459:Salicylates; D012503:Saponins; D013431:Sulfates; D013861:Thiocyanates; D012964:Sodium; D000251:Adenosine Triphosphatases; D008274:Magnesium; D011188:Potassium