title: Studies on delayed hypersensitivity pleural exudates in guinea pigs. I. Demonstration of substances in the cell-free exudate which cause inhibition of mononuclear cell migration in vitro.
abstract: Leucocyte migration inhibitory (MI) activity was investigated in vitro in the delayed hypersensitivity reaction induced by intrapleural injection of PPD into FCA-sensitized guinea-pigs. During the initial reaction (6 h) two types of antigen-dependent MI activity were detected in serum and cell-free exudate. One was of high molecular weight and associated with immunoglobulin and the other was of low molecular weight and appeared to be related to so-called "antigen-dependent MIF". As the reaction progressed (i.e. 12-24 h), two types of antigen-dependent MI activity were revealed in exudate, but not in serum. One was high molecular weight and the other was low molecular weight and thought to be related to so-called "antigen-independent MIF". Similar experiments were performed on the reverse passive Arthus reaction in the pleural cavity of guinea-pigs. A high molecular weight MI activity was detected in 6-h cell-free exudate and was found to be antigen-independent. "So-called MIF" was not found in this reaction.
mesh_terms: D000818:Animals; D000941:Antigens; D001183:Arthus Reaction; D002464:Cell Migration Inhibition; D006168:Guinea Pigs; D006968:Hypersensitivity, Delayed; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G; D008263:Macrophage Migration-Inhibitory Factors / Q000032:analysis*; D008297:Male; D008970:Molecular Weight; D010996:Pleural Effusion / Q000276:immunology*; D014373:Tuberculin
chemical_list: D000941:Antigens; D007074:Immunoglobulin G; D008263:Macrophage Migration-Inhibitory Factors; D014373:Tuberculin