The effects of thymidine and its analogues on the differentiation of antibody-forming cells.
Thymidine (Tdr) and its analogues 5-fluorodeoxyuridine (FUdr) and 5-bromodeoxyuridine (BUdr) inhibited the development of antibody-producing cells (PFC) in cultures of explants of rabbit spleen stimulated with sheep red cells in vitro. They were effective in this respect, even at concentrations too low to inhibit cell division. The results indicated that Tdr had produced its effects by a selective action on DNA and RNA synthesis. In view of what is known about the mechanisms of action of Tdr and its analogues to interfere with DNA-dependent events in cells, these results supported the idea that DNA synthesis, unrelated to the needs for cell division, was required for the development of antibody-producing capacity by PFC in these cultures.
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D000921:Antibody-Producing Cells / Q000187:drug effects*; D001973:Bromodeoxyuridine / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D003609:Dactinomycin / Q000494:pharmacology; D005260:Female; D005467:Floxuridine / Q000494:pharmacology*; D007930:Leucine / Q000378:metabolism; D008297:Male; D011691:Puromycin / Q000494:pharmacology; D012313:RNA / Q000096:biosynthesis; D011817:Rabbits; D012756:Sheep; D013154:Spleen / Q000166:cytology; D013936:Thymidine / Q000494:pharmacology*; D014316:Tritium; D014529:Uridine / Q000378:metabolism
D005467:Floxuridine; D014316:Tritium; D003609:Dactinomycin; D011691:Puromycin; D012313:RNA; D004247:DNA; D001973:Bromodeoxyuridine; D007930:Leucine; D013936:Thymidine; D014529:Uridine