Restoration of detergent-inactivated adenosine triphosphatase activity of human prostatic fluid with concanavalin A.
A stimulation by concanavalian A OF Mg2+ -and Ca2+-dependent ATPase (ATP phosphohydrolase, EC 3.6.1.3) of human prostatic fluid has been observed after the enzyme system had been inactivated by a detergent such as 0.05% deoxycholate. The concanavalin A effect was specific since the positive effect was abolished in the presence o alpha-methyl-D-mannoside. Furthermore, the positive effect of concanavalin A was obtained with a low lectin concentration, equal to the concentration reported for optimal stimulation of other membrane enzymes.
D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002118:Calcium / Q000494:pharmacology; D003208:Concanavalin A / Q000494:pharmacology*; D003840:Deoxycholic Acid / Q000494:pharmacology*; D004789:Enzyme Activation; D006801:Humans; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D008766:Methylmannosides / Q000494:pharmacology; D011467:Prostate / Q000201:enzymology*
D008766:Methylmannosides; D003840:Deoxycholic Acid; D003208:Concanavalin A; D000251:Adenosine Triphosphatases; D008274:Magnesium; D002118:Calcium