Preparative study on the isolation of single sarcomeres from rabbit skeletal muscle.
Single sarcomeres were prepared from fresh rabbit myofibrils by digestion with a calcium-activated factor (CAF). The rabbit single sarcomere has functional properties quite similar to those of single sarcomeres obtained from chicken muscle by a usual method. Thus it was found that the single sarcomeres obtained by CAF digestion were useful as a muscle model, though they were not completely intact.
D000185:Actinin / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D008854:Microscopy, Electron; D008970:Molecular Weight; D009132:Muscles / Q000201:enzymology / Q000648:ultrastructure*; D010447:Peptide Hydrolases / Q000378:metabolism; D011817:Rabbits
D000185:Actinin; D010447:Peptide Hydrolases; D000251:Adenosine Triphosphatases