title: T- and B-areas in immune reactions. Volume changes in T and B cell compartments of the rat spleen following intravenous administration of a thymus-dependent (SRBC) and a thymus-independent (paratyphoid vaccin-endotoxin) antigen. A histometric study.
abstract: In the white pulp of rat spleens cell numbers were studied in the different compartments following intravenous administration of comparable doses of paratyphoid vaccine (PTV, thymus-independent) and sheep red blood cells (SRBC, thymus-dependent). In the periarteriolar lymphatic sheaths (PALS) both cell concentration and volume were measured. For the follicles and the marginal zone only volume was recorded in the first 5 days following antigen administration. Additionally, histologic observations were made. In the thymus-dependent area PTV caused oedema 12-24 hours after administration. Following SRBC administration an increase in lymphocyte numbers occurred until the second day, probably representing an influx of T cells from the recirculating pool. Both antigens gave rise to a plasmacellular reaction in the peripheral PALS (2nd-4th day). In the bone marrow-dependent areas a massive shift of medium-sized lymphocytes from the marginal zone to the follicles took place in the first 24 hours following PTV administration. These cells subsequently transformed into blasts. After SRBC (6 and 12 hours) only a few marginal zone lymphocytes seemed to migrate into the follicles. It is aruged that the endotoxin present in PTV is responsible for the fact that, following administration of the antigen, all marginal zone (B) cells responded. Endotoxin stimulation might provide a model for the fate of marginal zone cells stimulated by other agents, such as antigen (stimulating antigen binding B cells) or antigen-antibody complexes (stimulating Fc-receptor B cells).
mesh_terms: D000818:Animals; D000937:Antigen-Antibody Reactions; D001402:B-Lymphocytes; D002454:Cell Differentiation; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000276:immunology*; D007275:Injections, Intravenous; D007958:Leukocyte Count; D008297:Male; D010587:Phagocytosis; D051381:Rats; D012756:Sheep; D013154:Spleen / Q000166:cytology*; D013601:T-Lymphocytes; D013997:Time Factors; D014436:Typhoid-Paratyphoid Vaccines / Q000008:administration & dosage*
chemical_list: D014436:Typhoid-Paratyphoid Vaccines