Corneal cryo-preservation and its clinical application.
Shortage of fresh corneal tissue for keratoplasty and expensive equipment needed for cryo-preservation has confined the field of corneal transplantation. The development of a simple medium for storage of corneal tissue which is inexpensive, which requires no technical assistance, and which provides the surgeon with excellent tissue would be very helpful. Forty-five of 55 human corneas stored for up to seven days in a simple tissue culture medium remained clear and thin after keratoplasty. Corneal deturgescence rates of tissue stored in this medium compared favorably with those rates for fresh and cryopreserved tissue.
D000818:Animals; D003315:Cornea / Q000166:cytology; D016039:Corneal Transplantation; D003470:Culture Media; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004727:Endothelium / Q000166:cytology; D005615:Freezing; D006084:Graft Rejection; D006801:Humans; D009926:Organ Preservation; D011817:Rabbits; D012709:Serum Albumin / Q000494:pharmacology; D013395:Sucrose / Q000494:pharmacology; D013696:Temperature; D013997:Time Factors; D014019:Tissue Donors; D014021:Tissue Preservation / Q000379:methods; D014184:Transplantation, Homologous
D003470:Culture Media; D012709:Serum Albumin; D013395:Sucrose; D004121:Dimethyl Sulfoxide