Plasma membranes from experimental granulation tissue.
1. A procedure was developed for the preparation of plasma membranes from experimental granulation tissue of the rat without the addition of enzymes. The yield is better than 20% and the purification at least tenfold. 2. Values are given for the activities of 5'-nucleotidase, Na-+, k-+-activated Mg-2+dependent adenosine triphosphatase and leucine beta-naphthylamidase, for lipid composition, and for the gel-electrophoretic patterns of proteins and glycoporteins in the membrane preparations. 3. The plasma membranes from the mature granulation tissue contain proportionally more protein in the lipid phase, but the specific activities of 5'-nucleotidase and Na-+,K-+-activated Mg-2+-dependent adenosine triphosphatase are smaller than in the proliferating tissue. Certain differences were repeatedly observed in the gel-electrophoretic patterns of the developmental phases. 4. The plasma membranes from the granulation tissue were compared with those from rat peritoneal macrophages and from embryonic-chick tendon cells.
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000626:Aminopeptidases / Q000378:metabolism; D000818:Animals; D002455:Cell Division; D002458:Cell Fractionation / Q000379:methods; D002462:Cell Membrane / Q000032:analysis / Q000648:ultrastructure; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006023:Glycoproteins / Q000032:analysis; D006097:Granulation Tissue / Q000648:ultrastructure*; D007700:Kinetics; D008055:Lipids / Q000032:analysis; D008264:Macrophages; D008274:Magnesium; D008854:Microscopy, Electron; D009708:Nucleotidases / Q000378:metabolism; D011188:Potassium; D011506:Proteins / Q000032:analysis; D051381:Rats; D012964:Sodium
D006023:Glycoproteins; D008055:Lipids; D011506:Proteins; D012964:Sodium; D009708:Nucleotidases; D000626:Aminopeptidases; D000251:Adenosine Triphosphatases; D008274:Magnesium; D011188:Potassium