[Monolayer culture of pancreatic beta cells of newborn rats: Insulin secretion in vitro and attempt at beta-cell transplantation in experimental diabetes].
Partially purified beta cell monolayer cultures were prepared from the pancrease of neonatal Wistar rats by dissociating the cells with a trypsin-collagenase solution. The cultures were grown in medium 199 containing a 10% fetal calf serum and 100 or 300 mg/100 ml glucose. Insulin release from the primary cultures during 12 days was 15 to 20 microunit/culture/day when the cells were grown in the medium containing 300 mg/100 ml glucose. When glucose concentration in the medium was decreased from 300 to 100 mg/ml insulin release fell to 2--5 microunit/culture/day. Theophylline stimulated insulin release in a short-time experiment. Transplantation of a 6--8-day culture in diabetic rats reduced the blood glucose concentration for 1 to 2 days.
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D003470:Culture Media; D003921:Diabetes Mellitus, Experimental / Q000473:pathology / Q000503:physiopathology*; D005947:Glucose / Q000494:pharmacology; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000166:cytology / Q000378:metabolism; D016381:Islets of Langerhans Transplantation; D051381:Rats; D013268:Stimulation, Chemical; D013311:Streptozocin; D013806:Theophylline / Q000494:pharmacology; D014184:Transplantation, Homologous
D003470:Culture Media; D007328:Insulin; D013311:Streptozocin; D013806:Theophylline; D005947:Glucose
Monosloĭnaia kul'tura pankreaticheskikh beta-kletok novorozhdennykh krys: Sekretsiia insulina in vitro i popytka transplantatsii zhivotnym s eksperimental'nym diabetom.