Soluble high-molecular-weight E fragments in the plasmin-induced degradation products of cross-linked human fibrin.
1. The factor XIII-mediated cross-linked alpha chains in fibrin have no effect on the nature of the fragments released during the solubilization of fibrin by plasmin. 2. Besides the known D dimer and E fragments solubilized during the lysis of cross-linked fibrin, other fragments have been observed on sodium dodecyl sulphate-polyacrylamide gel electrophoresis which have a molecular weight of about 135 000. After prolonged plasmin digestion, these fragments (U fragments) were no longer evident on the gels and the high-molecular-weight E antigen was absent. It is assumed that the E antigen was associated with the U fragments. These fragments also cross-reacted with an anti-D serum. 3. The U fragments have been tentatively presumed to be a factor XIII-mediated cross-linked D-E complex since they degrade only after prolonged degradation with plasmin. Whereas it is known that the fibrin D dimer fragment contains the cross-linked gamma chain residues of the originating fibrin, the presumed covalent cross-linking of the D-E fragments has not been proved. 4. The presence of these high-molecular-weight fragments, containing the E antigen, in cross-linked human fibrin digests should be taken into account in the development of D dimer assays to monitor fibrin lysis in vivo.
D000818:Animals; D001665:Binding Sites; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D005337:Fibrin; D005341:Fibrinolysin; D006801:Humans; D007122:Immunoelectrophoresis; D046911:Macromolecular Substances; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis; D010958:Plasminogen; D011485:Protein Binding; D011817:Rabbits / Q000276:immunology; D013300:Streptokinase
D046911:Macromolecular Substances; D010446:Peptide Fragments; D005337:Fibrin; D010958:Plasminogen; D013300:Streptokinase; D005341:Fibrinolysin