Inhibition of ATP synthesis associated with 6-aminonicotinamide (6-AN) teratogenesis in rat embryos.
Pregnant rats were injected ip with 6 mg/kg 6-aminonicotinamide (6-AN) at day 12 of gestation. Embryos removed between 1 and 48 h later had reduced adenosine triphosphate (ATP) concentrations, of about 50% of control values. All fetuses examined near term were malformed. Nicotinamide (NAM, 100 mg/kg) given ip 1 h after 6-AN afforded protection: malformations occurred in only 15% of the survivors; and there was minimal ATP reduction, 15% below control values. NAM given 2 and 4 h after 6-AN produced intermediate ATP concentrations and malformation frequencies. Thus, there was a relation between the embryotoxic and ATP-depressant actions of 6-AN in day 12 rat embryos.
D015120:6-Aminonicotinamide; D000014:Abnormalities, Drug-Induced / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D000818:Animals; D004140:Dinitrophenols; D005260:Female; D005333:Fetus / Q000187:drug effects / Q000378:metabolism; D009536:Niacinamide / Q000031:analogs & derivatives*; D011247:Pregnancy; D051381:Rats; D013723:Teratogens
D004140:Dinitrophenols; D013723:Teratogens; D009536:Niacinamide; D015120:6-Aminonicotinamide; D000255:Adenosine Triphosphate