title: Development of spontaneous motility in chick embryos. The spinal and supraspinal component of the inhibitory effect of glycine and GABA.
abstract: Development of the effect of glycine and gamma-aminobutyric acid [GABA] on spontaneous motility was studied in 11- to 19-day-old chick embryos under normal conditions and after acute and chronic decapitation. Chronic decapitation was performed on the 2nd day of incubation. Glycine (100 mg/kg egg weight) and GABA (103 mg/kg egg weight) (applied onto the shell membrane) demonstrably inhibited spontaneous motility only from the 15th day of incubation, the inhibitory effect increasing with the embryo's age. When administered together in half doses, glycine and GABA completely inhibited spontaneous motility for the first time in 19-day-old embryos. Neither amino acid influenced depression of motility immediately after decapitation, but 24 and 48 hours after, in 17- and 19-day-old embryos, they had a paradoxical effect, i.e. they transiently activated motor activity and even caused motor paroxysms. After chronic decapitation, both glycine and GABA again had a mild, protracted inhibitory effect. A comparison of spontaneous motility in normal and chronically decapitated embryos showed that the role of supraspinal factors in spinal motor output increases significantly with development of the chick embryo from the 15th day of incubation and that inhibition of these supraspinal factors plays the decisive role in the effect of glycine and GABA.
mesh_terms: D000613:Aminobutyrates / Q000494:pharmacology*; D000818:Animals; D001921:Brain / Q000196:embryology*; D002642:Chick Embryo / Q000502:physiology*; D005998:Glycine / Q000494:pharmacology*; D006257:Head / Q000601:surgery; D009068:Movement / Q000187:drug effects*; D013116:Spinal Cord / Q000196:embryology*; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology*
chemical_list: D000613:Aminobutyrates; D005680:gamma-Aminobutyric Acid; D005998:Glycine