Resolution and reconstitution of ion-transport systems.
1. Oxidative phosphorylation was reconstituted with a mitochondrial proton pump (oligomycin-sensitive ATPase) and segments of the oxidation chain (cytochrome oxidase or DPNH-Q1 reductase). A proton pump of bacteriorhodopsin substituted for the respiratory chain components, giving rise to light-induced ATP formation. 2. Since oxidative phosphorylation has thus become a special case of the problem of ion translocation in general, we have investigated and reconsituted other pumps. The reconstituted Ca++ pump of sarcoplasmic reticulum consists of two factors, the Ca++-dependent ATPase and a heat-stable coupling factor. 3. Other information obtained from reconstitution experiments include the role of asymmetry in organized membranes and the specificity of protein-phospholipid interaction. 4. Purified preparations of Ca++-ATPase catalyze the formation of ATP from Pi and ADP in a stepwise reaction stoichiometric with the enzyme and dependent on Ca++.
D000244:Adenosine Diphosphate / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000096:biosynthesis; D001436:Bacteriorhodopsins / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D002338:Carotenoids / Q000378:metabolism*; D002474:Cell-Free System; D006217:Halobacterium; D006358:Hot Temperature; D007477:Ions; D008027:Light; D008928:Mitochondria / Q000378:metabolism; D008954:Models, Biological; D010085:Oxidative Phosphorylation; D010710:Phosphates / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D011188:Potassium / Q000378:metabolism; D011522:Protons; D012964:Sodium / Q000378:metabolism
D007477:Ions; D010710:Phosphates; D010743:Phospholipids; D011522:Protons; D002338:Carotenoids; D001436:Bacteriorhodopsins; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D012964:Sodium; D000251:Adenosine Triphosphatases; D011188:Potassium; D002118:Calcium