Apparent sulfation of glycosaminoglycans by ascorbic acid 2-[3 5-S] sulfate: an explanation.
The sulfation of glycosaminoglycans by ascorbic acid 2-[35S]sulfate was studied in costal cartilage and chondrocytes in vitro. Negligable (if any) sulfation of glycosaminoglycans was detected with immediately isolated ascorbic acid 2-[35S]sulfate. However, formation of [35S]glycosaminoglycans was readily detected with ascorbic acid 2-[35S]sulfate which had been stored at minus 20 degrees C for several days. The [35S]glycosaminoglycans did not result from the direct transfer of 35S from ascorbic acid 2-sulfate but rather from a decomposition product of ascorbic acid 2-[35S]sulfate. Evidence is presented to show that the sulfation pathway with the decomposition product involves exchange with inorganic sulfate, and strongly suggests that sulfation proceeds via 3'-phosphoadenosine 5'-phosphosulfate. The decomposition product appears similar to inorganic sulfate in several test systems. In view of these observations, it is suggested that previous conclusions implicating as acid 2-sulfate as a biological sulfate donor, based on the use of ascorbic acid 2-[35S]sulfate be re-evaluated.
D000227:Adenine Nucleotides / Q000378:metabolism; D000818:Animals; D001205:Ascorbic Acid / Q000378:metabolism*; D002356:Cartilage / Q000187:drug effects / Q000378:metabolism*; D004355:Drug Stability; D005069:Evaluation Studies as Topic; D005233:Fatty Alcohols / Q000378:metabolism; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D008297:Male; D051381:Rats; D012272:Ribs; D013431:Sulfates / Q000378:metabolism* / Q000494:pharmacology; D013462:Sulfur Radioisotopes
D000227:Adenine Nucleotides; D005233:Fatty Alcohols; D006025:Glycosaminoglycans; D013431:Sulfates; D013462:Sulfur Radioisotopes; D001205:Ascorbic Acid