Comparison of plasma membranes and endoplasmic reticulum fractions obtained from whole white adipose tissue and isolated adipocytes.
The influence of the mode of preparation upon some of the characteristics of white adipose tissue plasma membranes and microsomes has been reported. Plasma membrane fractions prepared from mitochondrial pellet were shown to have higher specific activities of (Mg2+ + Na+ + K+)-ATPase than plasma membranes originating in crude microsomes. Isolation of fat cells by collagenase treatment was found to result in a decrease in specific activity of the plasma membrane enzymes; in plasma membranes prepared from isolated fat cells, the specific activity values obtained for (Mg2+ + Na+ +k+)-ATPase and 5'-nucleotidase were only 42% and 6.3% respectively of those obtained in plasma membranes prepared from whole adipose tissue. Purification of whole adipose tissue crude microsomes by hypotonic treatment caused extensive solubilization of the endoplasmic reticulum marker enzymes, NADH oxidase and NADPH cytochrome c reductase. The lability of endoplasmic reticulum marker enzymes, however, was found to be greatly diminished in the preparations from isolated fat cells. The possibility that NADH oxidase and NADPH cytochrome c reductase activities found in the plasma membranes are microsomal enzymes adsorbed by the plasma membranes is discussed. The peptide patterns as well as the NADH oxidase and NADPH cytochrome c reductase activity patterns of plasma membranes and purified microsomes were compared by means of sodium dodecyl sulfate or Triton X-100 polyacrylamide gel electrophoresis.
D000251:Adenosine Triphosphatases / Q000032:analysis; D000273:Adipose Tissue / Q000166:cytology / Q000201:enzymology / Q000648:ultrastructure*; D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D002462:Cell Membrane / Q000201:enzymology / Q000648:ultrastructure*; D004721:Endoplasmic Reticulum / Q000201:enzymology / Q000648:ultrastructure*; D066298:In Vitro Techniques; D008297:Male; D008861:Microsomes / Q000032:analysis / Q000201:enzymology; D009247:NADH, NADPH Oxidoreductases / Q000032:analysis; D009251:NADPH-Ferrihemoprotein Reductase / Q000032:analysis; D009708:Nucleotidases / Q000032:analysis; D011506:Proteins / Q000032:analysis; D051381:Rats
D011506:Proteins; D009247:NADH, NADPH Oxidoreductases; D009251:NADPH-Ferrihemoprotein Reductase; D009708:Nucleotidases; D000251:Adenosine Triphosphatases