Allosteric activation and competitive inhibition of yeast phosphofructokinase by d-fructose.
Purified phosphofructokinase from bakers yeast is activated by D-fructose in low concentrations (up to 1 mM) and inhibited by high concentrations. The stimulatory effect of D-fructose is similar, but smaller than that of AMP. In the presence of AMP (0.4 mM or higher) D-fructose does no longer stimulate, but its inhibitory effect persists (KI = 8 mM). Its dualistic action on phosphofructokinase activity indicates that D-fructose might induce low frequency in glycolytic oscillations by direct interaction with the enzyme.
D000249:Adenosine Monophosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000494:Allosteric Regulation; D001667:Binding, Competitive; D004789:Enzyme Activation; D005632:Fructose / Q000378:metabolism*; D005636:Fructosephosphates / Q000378:metabolism; D006019:Glycolysis; D010732:Phosphofructokinase-1 / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
D005636:Fructosephosphates; D005632:Fructose; D000249:Adenosine Monophosphate; D000255:Adenosine Triphosphate; D010732:Phosphofructokinase-1