Reversible inhibition of (Na+ + K+)-ATPase with a cardiac glycoside.
The effect of a semisynthetic cardiac glycoside, Actinogen (Ay22241), on Na+ + K+ - ATPase was studied. Ay22241 was found to be as an effective inhibitor of the enzyme as ouabain, Ay22241 inhibition was a time dependent process and was completely reversible. While ouabain inhibition was also time dependent, it was only partially reversible. This reversibility with Ay22241 should make it a useful tool in studying the mode of action of cardiac glycosides.
D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors*; D002297:Cardanolides / Q000494:pharmacology; D002298:Cardenolides; D002301:Cardiac Glycosides / Q000494:pharmacology*; D008274:Magnesium; D010042:Ouabain / Q000494:pharmacology; D011188:Potassium; D012964:Sodium
D002297:Cardanolides; D002298:Cardenolides; D002301:Cardiac Glycosides; D010042:Ouabain; C006277:actodigin; D012964:Sodium; D000251:Adenosine Triphosphatases; D008274:Magnesium; D011188:Potassium