Immunogenicity of human B cell antigens solubilized from cultured human lymphoid cells.
Antigens solubilized from culured human lymphoid cells WI-L2 and RPMI 1788 were partially purified by ultracentrifugation on a KBr gradient. These antigens injected into rabbits produced xenoantibodies which after absorption with melanoma cells became specific to B cell antigens. Three such xenoantisera were submitted to the Second Histocompatibility Workshop of the Americas and reacted much like alloantisera to B cell antigens against a large panel of B peripheral lymphocytes and cells from patients with chronic lymphocytic leukemia. Xenoantisera to B cell antigens inhibited the mixed lymphocyte reaction, but did not affect the mitogenic activity of phytohemagglutinin or the functional properties of C3 receptors, monkey red blood cell receptors, or T cell receptors.
D000906:Antibodies; D000917:Antibody Formation; D000941:Antigens; D001402:B-Lymphocytes / Q000276:immunology*; D002460:Cell Line; D003601:Cytotoxicity Tests, Immunologic; D006680:HLA Antigens; D006801:Humans; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D014461:Ultracentrifugation
D000906:Antibodies; D000941:Antigens; D006680:HLA Antigens