Purification of histone F3 by covalent chromatography.
The arginine-rich histone F3 has been purified by covalent affinity chromatography. By the use of activated Thiol-Sepharose 4B for the purification of cysteine containing histone F3 a highly purified protein was obtained. The simple purification procedure offers the opportunity to get larger amounts of pure histone F3 within short time.
D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000302:isolation & purification*; D013438:Sulfhydryl Compounds; D013950:Thymus Gland / Q000032:analysis
D006657:Histones; D013438:Sulfhydryl Compounds