Macrophage metabolism during phagocytosis and digestion of normal and IgG antibody-coated sheep erythrocytes.
The respiration rate, aerobic glycolysis and glycolytic enzyme activities of purified mouse peritoneal macrophages increase during phagocytosis of either free or antibody-coated SRBC. These changes, however, seem to be roughly parallel to the amount of phagocytized antigen. In the digestive phase, Ag-Ab complexes induce more marked changes in macrophage metabolism than a comparable amount of free antigen. Lactate production, activity of several glycolytic enzymes and alanine-aminotransferase as well as glycogen content are greatly affected. Since in cell transfer experiments macrophage-associated SRBC can prime normal recipients for antibody response, whereas cell-associated SRBC-antibody complexes cannot, it is concluded that the changed macrophage metabolism drives antibody-coated antigen into metabolic channels allowing its rapid and total degradation.
D000332:Aerobiosis; D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D001666:Binding Sites, Antibody; D004912:Erythrocytes / Q000276:immunology; D006019:Glycolysis; D006593:Hexokinase / Q000378:metabolism; D007074:Immunoglobulin G; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D010101:Oxygen Consumption; D010587:Phagocytosis; D010732:Phosphofructokinase-1 / Q000378:metabolism; D011770:Pyruvate Kinase / Q000378:metabolism; D012756:Sheep / Q000276:immunology; D013997:Time Factors
D007074:Immunoglobulin G; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D006593:Hexokinase; D010732:Phosphofructokinase-1; D011770:Pyruvate Kinase