title: The effect of temperature change on the kinetic behaviour of yeast phosphofructokinase.
abstract: Yeast phosphofructokinase does not exhibit any cold sensitivity. The kinetic properties of the enzyme have been investigated in the range between 10 degrees C and 30 degrees C in dependence on fructose 6-phosphate and ATP. Although a significant increase in the enzyme activity with rising temperature does not occur, the shape of the ATP velocity curves is not markedly altered. With increasing concentrations of fructose-6-phosphate the efficiency of temperature on the catalytic process increases, indicating a small temperature effect on the shape of the fructose-6-phosphate velocity curves. The results are interpreted in terms of an adequate kinetic model.
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002384:Catalysis; D002645:Chickens; D003080:Cold Temperature; D007700:Kinetics; D008954:Models, Biological; D010732:Phosphofructokinase-1 / Q000378:metabolism*; D011817:Rabbits; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D013696:Temperature
chemical_list: D000255:Adenosine Triphosphate; D010732:Phosphofructokinase-1