Biochemical and ultrastructural correlates of substrate stimulation of renal organic anion transport.
Penicillin pretreatment enhanced the rate of PAH uptake into separated proximal tubules (collagenase digestion) from 2-week New Zealand white rabbits. A double reciprocal plot of these data suggests that penicillin increases the maximal velocity of PAH uptake. Na, K-ATPase was less in adult tissue but was unaffected by penicillin. No ultrastructural changes could be attributed to the treatment. Thus substrate stimulation of PAH transport does not involve Na, K-ATPase and probably involves soluble, rather than structural proteins.
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000367:Age Factors; D000618:Aminohippuric Acids / Q000378:metabolism*; D000818:Animals; D000831:Animals, Newborn; D000838:Anions; D001693:Biological Transport, Active / Q000187:drug effects; D066298:In Vitro Techniques; D007687:Kidney Tubules, Proximal / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure; D007700:Kinetics; D010402:Penicillin G Procaine / Q000494:pharmacology*; D011817:Rabbits
D000618:Aminohippuric Acids; D000838:Anions; D010402:Penicillin G Procaine; D000251:Adenosine Triphosphatases