title: Crossed immunoelectrophoresis of bovine milk fat globule membrane protein solubilized with non-ionic detergent.
abstract: Detergent solubilized bovine milk fat globule membrane material studied by crossed immunoelectrophoresis combined with histochemical techniques revealed four major protein complexes. All four were found to bind to concanavalin A and three were identified as sialoglycoproteins. Xanthine oxidase activity was associated with the non-sialoglycoprotein precipitate. Immunoabsorption with intact milk fat globules showed an internal location of the xanthine oxidase, whereas the three other main proteins plus Mg2+-ATPase and 5'-nucleotidase were disposed on the outer membrane surface. The major proteins from milk fat globule membrane and membrane material isolated from skim milk showed immunochemical identity.
mesh_terms: D000251:Adenosine Triphosphatases / Q000032:analysis; D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000276:immunology; D003208:Concanavalin A / Q000378:metabolism; D003902:Detergents; D005260:Female; D007123:Immunoelectrophoresis, Two-Dimensional; D008565:Membrane Proteins / Q000276:immunology*; D008892:Milk / Q000276:immunology*; D008894:Milk Proteins / Q000276:immunology; D009708:Nucleotidases / Q000032:analysis; D011485:Protein Binding; D012795:Sialoglycoproteins / Q000032:analysis; D014969:Xanthine Oxidase / Q000032:analysis
chemical_list: D003902:Detergents; D008565:Membrane Proteins; D008894:Milk Proteins; D012795:Sialoglycoproteins; D003208:Concanavalin A; D014969:Xanthine Oxidase; D009708:Nucleotidases; D000251:Adenosine Triphosphatases