Functional characteristics of BALB/c T cell lines: suppression in the mixed lymphocyte response and cell-mediated lysis.
Previously, 10 BALB/c T cell lines (Thy 1.2+, Ig-) were shown to express different combinations of Ly 1 and Ly 2 antigens. The possible immunologic function(s) of these tumor cells was determined by investigating the effects of these cells on the responses to mitogens, the mixed lymphocyte response (MLR), and the generation of cell-mediated lysis (CML) by normal spleen cells. Five T cell lines, P1798 and BALENTL 3, 5, 8, and 9, continued to synthesize DNA after exposure to large doses of irradiation. Only BALENTL 4, 6, 7, and 14 (Ly 1-(2+)) and BALENTL 13 (Ly 1+(2-)) were radiosensitive and therefore amenable to study. BALENTL 4 and 14 gave significant suppression of the MLR between BALB/c and C57BL/6; BALENTL 14 also inhibited the generation of BALB/c effector cells against C57BL/6 spleen cells. None of these T cell lines had any effect on the proliferative response of BALB/c spleen cells induced by concanavalin A. However, there was approximately 50% suppression of the phytohemagglutinin response of BALB/c spleen cells by BALENTL 14.
D000818:Animals; D002460:Cell Line; D003208:Concanavalin A / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic; D005260:Female; D007111:Immunity, Cellular; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008223:Lymphoma / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010835:Phytohemagglutinins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology* / Q000528:radiation effects; D013936:Thymidine / Q000378:metabolism
D010835:Phytohemagglutinins; D003208:Concanavalin A; D013936:Thymidine