Isolation fo 15alpha-hydroxypregnenolone and 15alpha-hydroxydehydroisoandrosterone from human pregnancy urine.
15alpha-Hydroxydehydroisoandrosterone and 15alpha-hydroxypregnenolone were isolated from hydrolyzed extracts of human late pregnancy urine and identified by means of the isotope dilution technique. In two separate determinations the excretion rate of 15alpha-hydroxydehydroisoandrosterone was found to be 1.7 and 3.2 microgram per day while that of 15alpha-hydroxypregnenolone was 1.7 and 2.9 microgram per day. It is postulated that 15alpha-hydroxydehydroisoandrosterone might serve as a precursor of 15alpha-hydroxylated estrogens already isolated from late pregnancy urine. Similarly, 15alpha-hydroxypregnenolone might be an endogenous precursor of 15alpha-hydroxyprogesterone.
D006907:17-alpha-Hydroxypregnenolone / Q000652:urine*; D002250:Carbon Radioisotopes; D002854:Chromatography, Paper; D002855:Chromatography, Thin Layer; D003687:Dehydroepiandrosterone / Q000031:analogs & derivatives* / Q000652:urine; D005260:Female; D006801:Humans; D011247:Pregnancy; D011263:Pregnancy Trimester, Third; D011865:Radioisotope Dilution Technique; D014316:Tritium
D002250:Carbon Radioisotopes; D014316:Tritium; D006907:17-alpha-Hydroxypregnenolone; D003687:Dehydroepiandrosterone