title: [Control of glycolysis in magnesium deficiency: studies on intact red cells and hemolysates].
abstract: The behaviour of glycolytic flux and glycolytic metabolic concentrations was studied under conditions of magnesium deficiency. The Mg-deficiency was produced in whole animals (rats) by feeding a diet almost completely free of Mg and in hemolysates of men by the addition of a chelating agent. The results show that the decrease of the free Mg-level is diminished by partial destruction of ATP and 2,3-DPG. The analysis of the control strength of the overall flux leads to the conclusion that the decrease of the glycolytic rate is caused by an inhibition of the hexokinase-phosphofructokinase-control system. The decrease of the MgATP-Complex and free Mg++-level explains the diminished phosphorylation of glucose by the hexokinase. The ATP-inhibition of the phosphofructokinase is amplified by a small increase of free ATP-concentration and a simultaneous decrease of the Fru-6P-level. The increase of the PEP-level is caused by the diminished free Mg++ and MgATP-complex and does not demonstrate a larger control strength of the pyruvate kinase.
mesh_terms: D000244:Adenosine Diphosphate / Q000097:blood; D000255:Adenosine Triphosphate / Q000097:blood; D000818:Animals; D004912:Erythrocytes / Q000378:metabolism*; D006019:Glycolysis; D006593:Hexokinase / Q000097:blood; D066298:In Vitro Techniques; D008275:Magnesium Deficiency / Q000097:blood* / Q000201:enzymology; D010732:Phosphofructokinase-1 / Q000097:blood; D011770:Pyruvate Kinase / Q000097:blood; D051381:Rats
chemical_list: D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D006593:Hexokinase; D010732:Phosphofructokinase-1; D011770:Pyruvate Kinase
vernacular_title: Kontrolle der Glykolyse bei magnesiummangel: Untersuchungen an intakten roten Blutzellen und Hämolysaten.