Purification and some characteristics of liver cytosol cornin, an antimitotic substance from rat liver cytosol.
Further purification and characterization are reported on rat cytosol cornin (RLCC), an antimitotic substance. Fraction I (purified RLCC) was purified more than 10-fold from crude RLCC with Sephadex G-50 column chromatography and showed a remarkable inhibitory effect on division of inseminated sea urchin eggs and mouse fibroblast cells. Fraction I was observed as one spot, and the molecular weight was estimated to be about 25,000 by thin layer gel filtration. Fraction I contained protein (92%) and RNA (8%), but the antimitotic activity was scarcely affected by treatment by pancreatic RNase. The protein of Fraction I was separated into two bands by SDS-polyacrylamide gel electrophoresis, and the molecular weight was estimated as 10,000 and 15,000, respectively. The 50% inhibition dose of Fraction I on the first division of inseminated sea urchin eggs and on proliferation of mouse L cells was about 2.5 X 10(-5) g/ml and 5 X 10(-4) g/ml, respectively. The yield of fraction I was about 35 mg from 100 g rat liver.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D003600:Cytosol / Q000032:analysis*; D005260:Female; D006133:Growth Substances / Q000032:analysis*; D008099:Liver / Q000032:analysis*; D008297:Male; D008938:Mitosis / Q000187:drug effects*; D009711:Nucleotides / Q000032:analysis; D010063:Ovum / Q000166:cytology; D010455:Peptides / Q000032:analysis; D051381:Rats; D012617:Sea Urchins
D000596:Amino Acids; D006133:Growth Substances; D009711:Nucleotides; D010455:Peptides