title: [The role of distinct membrane components of the enzyme in the ouabain sensitivity of Na+/K+ ATPase].
abstract: Purified plasma membranes from a cell line derived from the murine plasmocytoma MOPC 173 exhibited a 50% inhibition of the Na+K+ ATPase by 120 muM ouabain. EDTA treatment of such membranes induced a drop in the ouabain concentration needed for 50% inhibition to 0.4 muM. Supernatants obtained after EDTA treatment were able to complement with Ca(++) + Mg++ the washed EDTA treated membranes which recover their original resistance.
mesh_terms: D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors; D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology* / Q000648:ultrastructure; D008563:Membrane Lipids / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D008861:Microsomes / Q000201:enzymology; D010042:Ouabain / Q000494:pharmacology*; D013329:Structure-Activity Relationship
chemical_list: D008563:Membrane Lipids; D008565:Membrane Proteins; D010042:Ouabain; D000251:Adenosine Triphosphatases
vernacular_title: Rôle de constituants membranaires distincts de l'enzyme dans la sensibilité de l'ATPase Na+/K+ a l'ouabaïne