Functional role of soluble mitochondrial ATPase subunits.
A preparation of soluble mitochondrial ATPase (coupling factor F1) containing no gamma and delta minor subunits has been isolated. The minor-subunits-deficient F1 was found to be competent in ATP hydrolysis. However, it did not demonstrate a "coupling" effect in EDTA-submitochondrial particles. A portion of the ATPase activity of EDTA particles, stimulated by the minor-subunits-deficient F1, was insensitive to oligomycin. ATPase activity of Na+-particles was changed only slightly by this F1. It is suggested that gamma and delta subunits are necessary to form specific contacts between the F1 molecule and components of the mitochrondrial membrane.
D000251:Adenosine Triphosphatases / Q000378:metabolism; D008566:Membranes / Q000201:enzymology; D008931:Mitochondria, Muscle / Q000201:enzymology*; D009206:Myocardium / Q000648:ultrastructure; D009840:Oligomycins / Q000494:pharmacology; D010085:Oxidative Phosphorylation; D013329:Structure-Activity Relationship
D009840:Oligomycins; D000251:Adenosine Triphosphatases