title: The isolated heavy chain of an Acanthamoeba myosin contains full enzymatic activity.
abstract: Acanthamoeba myosin IB is a single-headed enzyme containing one heavy chain of 125,000 daltons, one light chain of 27,000 daltons, and one light chain of 14,000 daltons. The 125,000- and 27,000-dalton polypeptides are consistently found in a molar ratio of 1:1. The content of the 14,000-dalton peptide is usually only 0.1 to 0.2, and always less than 0.5, relative to the other two chains and might be a contaminant or a degradation product of one of the other chains. The specific activities of the Ca2+-ATPase, (K+, EDTA)-ATPase, and (after phosphorylation of its heavy chain by a specific kinase) actin-activated Mg2+-ATPase of Acanthamoeba myosin IB are similar to those of rabbit skeletal muscle myosin. After treatment of the enzyme with 2 M LiCl, the 125,000-dalton heavy chain of Acanthamoeba myosin Ib can be obtained, by chromatography on Sephadex G-200, essentially free of the 14,000-dalton peptide and more than 90% free of the 27,000-dalton peptide. This isolated heavy chain has the same specific ATPase activities as the original enzyme. Therefore, the heavy chain of Acanthamoeba myosin IB contains the ATPase catalytic site, the actin-binding site, and the phosphorylation site and is fully active enzymatically in the absence of light chains.
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000656:Amoeba / Q000201:enzymology*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D004789:Enzyme Activation; D008274:Magnesium / Q000378:metabolism; D008970:Molecular Weight; D009218:Myosins / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism
chemical_list: D000251:Adenosine Triphosphatases; D009218:Myosins; D008274:Magnesium; D011188:Potassium; D002118:Calcium