title: Affinity chromatographic preparation of arterial heavy meromyosin subfragment-1.
abstract: Heavy meromyosin subfragment-1 (HMM S-1) was prepared by papain digestion of arterial myosin or actomyosin and was purified by agarose-ATP affinity chromatography. Proteolysis of crude arterial myosin suspensions was preceded by solubilization. HMM-S-1 thus obtained consisted mainly of a 90,000 dalton polypeptide and fully retained the K+- and Ca2+-ATPase of the parent myosin. Its affinity to agarose-ATP was comparable to that of skeletal muscle HMM S-1.
mesh_terms: D000251:Adenosine Triphosphatases / Q000302:isolation & purification; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002339:Carotid Arteries / Q000032:analysis*; D002846:Chromatography, Affinity; D008970:Molecular Weight; D015879:Myosin Subfragments / Q000302:isolation & purification*; D010206:Papain; D010446:Peptide Fragments / Q000302:isolation & purification; D011188:Potassium / Q000494:pharmacology
chemical_list: D015879:Myosin Subfragments; D010446:Peptide Fragments; D010206:Papain; D000251:Adenosine Triphosphatases; D011188:Potassium; D002118:Calcium