[Isolation and properties of the cell envelope of the green alga Chlorella fusca (author's transl)].
Chlorella cell envelopes were isolated by a mechanical procedure. The obtained morphological structure shown in an EM-picture has a mean density of rho=1.42. A characteristic chemical property of the envelope is the percentage of mono- and di-unsaturated C18-fatty acids which is considerably higher than that of intact cells. With 14C labelled sugars a selectivity of the hexose uptake could be shown, galactose uptake amounting only to about one fifth that of glucose and mannose. The uptake shows a temperature maximum. Part of the 14C registered after a 30 min uptake period is released by high concentration of unlabelled glucose, 20% are irreversibly fixed.
D002458:Cell Fractionation; D002473:Cell Wall / Q000032:analysis / Q000378:metabolism* / Q000648:ultrastructure; D002708:Chlorella / Q000378:metabolism*; D005227:Fatty Acids / Q000032:analysis; D005690:Galactose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006601:Hexoses / Q000378:metabolism*; D008358:Mannose / Q000378:metabolism; D013696:Temperature
D005227:Fatty Acids; D006601:Hexoses; D005947:Glucose; D008358:Mannose; D005690:Galactose
Isolierung und Eigenschaften der Zellhülle der Grünalge Chlorella fusca