title: Membrane mutation affecting energy-linked functions in Escherichia coli K 12.
abstract: A small-colony forming variant of Escherichia coli with a mutation in the ncf gene was analysed. The alternation of the protein composition in the cytoplasmic membrane and the interaction with K and E group colicins indicated a membrane mutation. The effect of this mutation on some membrane-bound processes, the activity of Mg2+-activated ATPase, the growth on different carbon sources and the active transport of amino acids, is described. This mutation does not exert any effect on the electron transport system.
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D000332:Aerobiosis; D000596:Amino Acids / Q000378:metabolism; D000693:Anaerobiosis; D001414:Bacitracin / Q000494:pharmacology; D001426:Bacterial Proteins / Q000096:biosynthesis; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D003087:Colicins / Q000494:pharmacology; D003840:Deoxycholic Acid / Q000494:pharmacology; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D005796:Genes; D005947:Glucose / Q000378:metabolism; D007773:Lactates / Q000378:metabolism; D008565:Membrane Proteins / Q000096:biosynthesis; D009154:Mutation; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D014640:Vancomycin / Q000494:pharmacology
chemical_list: D000596:Amino Acids; D001426:Bacterial Proteins; D003087:Colicins; D007773:Lactates; D008565:Membrane Proteins; D003840:Deoxycholic Acid; D001414:Bacitracin; D012967:Sodium Dodecyl Sulfate; D014640:Vancomycin; D000251:Adenosine Triphosphatases; D005947:Glucose