The solubility properties of granulopoiesis inhibiting factor.
The solubility of granulopoiesis inhibiting factor (GIF) in various aqueous and organic solvents was investigated. GIF is soluble in water, 10% acetic acid, and 10% pyridine. It is not extractable by 1-butanol at low and high pH. A high solubility was found in polar organic solvents (ethanol-acetone 9:1, and chloroform-methanol 1:1), whereas GIF seems to be insoluble in pure chloroform.
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects / Q000378:metabolism*; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006098:Granulocytes / Q000187:drug effects / Q000378:metabolism*; D006131:Growth Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology; D006410:Hematopoiesis; D066298:In Vitro Techniques; D007962:Leukocytes / Q000378:metabolism*; D051381:Rats; D012995:Solubility; D012997:Solvents; D013936:Thymidine / Q000378:metabolism*
D006131:Growth Inhibitors; D012997:Solvents; D013936:Thymidine