[Study of the extracellular macromolecular meshwork elaborated by rat aortic cells in secondary culture].
Rat aortic smooth muscle cells isolated by digestion of the vessels by elastase and trypsin and grown in subculture, are examinated by phase, optic and electron microscopy for their ability to synthesize connective tissue components. Large amounts of extracellular material accumulates within the spaces between the cell; it consists of amorphous substance identified histochemically as elastin, of 110 A microfibrils and of periodic fibrils (430-490 A); the chemical nature of these two last components is discussed.
D000818:Animals; D001011:Aorta / Q000378:metabolism / Q000648:ultrastructure*; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis; D004549:Elastin / Q000096:biosynthesis; D051381:Rats
D003094:Collagen; D004549:Elastin
Etude de la trame macromoléculaire extracellulaire élaborée par des cellules aortiques de rat en culture secondaire