title: Biological characteristics of a type I restriction-modification system in Staphylococcus aureus.
abstract: Two restriction-modification systems, S1 and S2, are present in Staphylococcus aureus RN450 (S. Iordanescu and M. Surdeanu, J. Gen. Microbiol., 96:277-281, 1976). System S2 affects phage multiplication after both infection and transfection. Unmodified plasmid and chromosomal DNAs are also not expressed following transduction and transformation into a restrictive host. Restricted phages are, however, capable of conferring phage-mediated competence, although the state of competence does not affect the restriction-modification system. The restricting activity of system S2 is inactivated by heat treatment of the cells. An enzymatic activity that restricts unmodified phage DNA in the presence of ATP, Mg2+, and S-adenosylmethionine was recovered from cell-free extracts of a strain RN450 derivative.
mesh_terms: D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004269:DNA, Bacterial / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism; D010957:Plasmids; D012436:S-Adenosylmethionine / Q000494:pharmacology; D013204:Staphylococcus Phages / Q000254:growth & development; D013211:Staphylococcus aureus / Q000201:enzymology* / Q000235:genetics; D014161:Transduction, Genetic; D014169:Transformation, Bacterial
chemical_list: D004269:DNA, Bacterial; D004279:DNA, Viral; D012436:S-Adenosylmethionine; D004262:DNA Restriction Enzymes