Phosphorylation of purine and pyrimidine nucleosides by isolated rat liver mitochondria.
Formation of 5'-AMP, 5'-GMP, 5'-CMP and 5'UMP was confirmed in isolated rat liver mitochondria incubated with alpha-ketoglutarate, inorganic phosphate, purine nucleoside and pyrimidine nucleoside. Increased incorporation of 32Pi into ATP, GTP and UTP was observed by adding purine- and pyrimidine nucleosides. The phosphorylation of nucleosides was inhibited severely by arsenite and affected slightly by the addition of nuclear or post-mitochondrial fraction.
D000818:Animals; D003597:Cytosine Nucleotides / Q000096:biosynthesis; D006150:Guanine Nucleotides / Q000096:biosynthesis; D066298:In Vitro Techniques; D008930:Mitochondria, Liver / Q000378:metabolism*; D011684:Purine Nucleosides / Q000378:metabolism*; D011741:Pyrimidine Nucleosides / Q000378:metabolism*; D051381:Rats; D014500:Uracil Nucleotides / Q000096:biosynthesis
D003597:Cytosine Nucleotides; D006150:Guanine Nucleotides; D011684:Purine Nucleosides; D011741:Pyrimidine Nucleosides; D014500:Uracil Nucleotides