title: [A modified method for labeling erythrocytes with 99mTc (author's transl)].
abstract: A modified method for labeling erythrocytes with 99mTc-sodium pertechnetate for spleenic scintigraphy is described. In vitro investigations have shown that by omitting the customary process of washing and by simultaneous alteration of the erythrocytes during the tin incubation period, the duration of erythrocyte labeling can be considerably reduced. A yield of 96 of 98% can be achieved. The effect of the tin on the erythrocytes is further investigated. The effect of the incubation period and temperature, of the plasma in the erythrocyte suspension, of stirring and agitation, of the dispersion liquid and tin concentration were studied. The method proves satisfactory in 56 controlled spleenic scintigrams.
mesh_terms: D004912:Erythrocytes; D007553:Isotope Labeling / Q000379:methods*; D010084:Oxidation-Reduction; D013667:Technetium; D013997:Time Factors; D014001:Tin
chemical_list: D013667:Technetium; D014001:Tin
vernacular_title: Ein modifiziertes Verfahren zur 99mTc-Markierung von Erythrozyten