title: [Use of immunochemical methods for characterization and purification of tissue-specific inhibitors of mitotic activity (chalones)].
abstract: The 81% ethanol-precipitable fraction of water-soluble proteins from skin inhibiting the proliferation of epidermal tissues was shown to contain 9 protein components according to acrylamide gel disk-electrophoresis. Seven of these were identical to homologous serum proteins and could be adsorbed on the corresponding immunosorbents. Two proteins remained in the solution after immunoadsorption. Their electrophoretic properties were the same as those in the total 81% ethanol fractions. These proteins like the 81% ethanol fraction inhibited the entering of cells into mitosis and DNA synthesis phase.
mesh_terms: D000818:Animals; D004247:DNA / Q000096:biosynthesis; D004431:Ear, External / Q000187:drug effects; D006131:Growth Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology; D007122:Immunoelectrophoresis; D008297:Male; D008938:Mitosis / Q000187:drug effects; D051381:Rats; D012867:Skin / Q000032:analysis*; D014059:Tongue / Q000187:drug effects
chemical_list: D006131:Growth Inhibitors; D004247:DNA
vernacular_title: Ispol'zovanie immunokhimicheskikh metod dlia kharakteristiki i ochistki tranespetsificheskikh ingibitorov mitoticheskoĭ aktivnosti (keĭlonov)