Properties of the scrapie agent-endomembrane complex from hamster brain.
Subcellular fractionation of scrapie-infected hamster brain indicated the association of the scrapie agent with a component of the endomembrane system. Characterization by equilibrium density gradient centrifugation, electron microscopy, and marker enzymes suggested a primary association with rough and smooth endoplasmic reticulum and a possible incorporation into the plasma membrane. DNA polymerase activity demonstrated a direct correlation with regions of scrapie activity from the gradient fractions. A scrapie-related product was detected after (3H)TMP incorporation and analysis on 2.2% polyacrylamide gels. Analysis of nucleic acid species extracted from subcellular fractions resulted in a greater quantity from healthy brain; however, no qualitative distinctions were detected.
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000201:enzymology / Q000382:microbiology*; D002462:Cell Membrane / Q000201:enzymology / Q000382:microbiology; D006224:Cricetinae; D003579:Cytochrome Reductases / Q000378:metabolism; D004254:DNA Nucleotidyltransferases / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000201:enzymology / Q000382:microbiology*; D009696:Nucleic Acids / Q000032:analysis; D011328:Prions / Q000032:analysis / Q000648:ultrastructure; D013347:Subcellular Fractions; D014764:Viral Proteins / Q000032:analysis
D009696:Nucleic Acids; D011328:Prions; D014764:Viral Proteins; D003579:Cytochrome Reductases; D004254:DNA Nucleotidyltransferases; D000251:Adenosine Triphosphatases