title: Analysis of urinary mucopolysaccharides using small ion exchange columns.
abstract: Methods are presented for the small scale column chromatography of urinary mucopolysaccharides. After isolation by cety pyridinium chloride mucopolysaccharides are applied to a small column of ion exchange resin and eluted by 2 M-sodium chloride. Colorimetric assays on this elutate allow clinically significant disease states to be detected.
mesh_terms: D011232:Chemical Precipitation; D002852:Chromatography, Ion Exchange; D005688:Galactosamine / Q000032:analysis; D005944:Glucosamine / Q000032:analysis; D006025:Glycosaminoglycans / Q000652:urine*; D006801:Humans; D006821:Hyaluronoglucosaminidase; D008722:Methods; D009083:Mucopolysaccharidoses / Q000652:urine; D012965:Sodium Chloride; D014574:Uronic Acids / Q000032:analysis
chemical_list: D006025:Glycosaminoglycans; D014574:Uronic Acids; D012965:Sodium Chloride; D005688:Galactosamine; D006821:Hyaluronoglucosaminidase; D005944:Glucosamine