Generation of T memory cells in one-way mixed lymphocyte culture. IV. Primary and secondary responses to soluble and insoluble membrane preparations and to ultraviolet-light-inactivated stimulator cells.
Neither normal CBA (H-2k) nor purified spleen T cells respond in vitro to soluble or insoluble membrane preparations or to ultraviolet-light-inactivated stimulator cells of the allogeneic DBA/2 (H-2d) strain. However, CBA spleen cells deprived of phagocytic cells show a slight proliferative response under these conditions. After being primed against mitomycin-blocked DBA/2 cells in one-way mixed lymphocyte culture, the secondary blast-derived T 'memory' cells display a good secondary blast (proliferative) response to both membrane antigens and to ultraviolet-light-inactivated stimulator cells. In addition to this, the secondary T lymphocytes--in contrast to nonprimed T cells--respond by cytotoxicity when ultraviolet-light-inactivated cells are used as the second stimulant.
D000818:Animals; D000941:Antigens; D002462:Cell Membrane / Q000276:immunology*; D003429:Cross Reactions; D007156:Immunologic Memory; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D008937:Mitomycins / Q000494:pharmacology; D011830:Radiation Effects; D013154:Spleen / Q000187:drug effects / Q000276:immunology / Q000528:radiation effects; D013601:T-Lymphocytes / Q000276:immunology*; D013997:Time Factors; D014466:Ultraviolet Rays
D000941:Antigens; D008937:Mitomycins