Measurement of urinary mucopolysaccharides.
The fact that the polyanionic long chains of mucopolysaccharides (MPS) can be precipitated with detergents such as cetylpiridiniumchloride (CPC) has been used in the development of a method for the quantitative estimation of total MPS in urine. Urine samples are all brought to an osmolarity of 300, after which an equal volume of 0,1% CPC is added to each sample. The turbidity is determined spectrophotometrically after 5 minutes. Chondroitin sulphate A was used as an internal standard. Normal values obtained for different age groups in the population of Pretoria are discussed.
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D002594:Cetylpyridinium; D002624:Chemistry, Clinical / Q000379:methods; D002648:Child; D002675:Child, Preschool; D003404:Creatinine / Q000652:urine; D005260:Female; D006025:Glycosaminoglycans / Q000652:urine*; D006801:Humans; D008297:Male; D009391:Nephelometry and Turbidimetry; D009994:Osmolar Concentration; D014556:Urine
D006025:Glycosaminoglycans; D003404:Creatinine; D002594:Cetylpyridinium