Influence of mixtures of taurocholate, fatty acids, and monolein on the toxic effects of deoxycholate in rat jejunum in vivo.
The influence of mixtures of taurocholate (TC), oleic acid (OA), caprylic acid (CA), and monolein (MO) on the toxic effects of deoxycholate (DC) in rat jejunum have been investigated using both a closed loop and perfusion technique. DC induced net secretion of water and electrolytes, inhibited glucose transport and transmural potential difference (PD), and inactivated mucosal "total" and (Na+ -K+)-adenosine triphosphatase. Secretion was reversed to absorption when the instilled or perfused solutions were composed of mixtures of DC, TC and OA; substitution of MO or CA for OA produced a similar effect. DC-induced inhibition of PD, glucose absorption, and mucosal adenosine triphosphatase activity was abolished when DC was mixed with TC and OA. Oleic acid emulsions had no effect on secretion induced by DC. Absorption of DC was inhibited from mixed micellar solutions (TC, OA, DC) but not from pure micellar solutions (TC, DC). These results indicate that the presence of taurocholate and fatty acids or monolein within the intestinal lumen markedly modify a number of the toxic effects of DC on jejunal function. The clinical effects of DC on intestinal function in man may therefore depend on the relative concentrations of other bile salts and lipids within the intestinal lumen.
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002210:Caprylates / Q000494:pharmacology*; D003840:Deoxycholic Acid / Q000494:pharmacology / Q000633:toxicity*; D004347:Drug Interactions; D005947:Glucose / Q000378:metabolism; D007408:Intestinal Absorption / Q000187:drug effects; D007413:Intestinal Mucosa / Q000378:metabolism; D007583:Jejunum / Q000187:drug effects* / Q000502:physiology; D008297:Male; D009829:Oleic Acids / Q000494:pharmacology*; D051381:Rats; D013656:Taurocholic Acid / Q000494:pharmacology*
D002210:Caprylates; D009829:Oleic Acids; D003840:Deoxycholic Acid; D013656:Taurocholic Acid; D000251:Adenosine Triphosphatases; D005947:Glucose