Behaviour of sheep-immunoglobulin-bearing and non-immunoglobulin-bearing lymphocytes isolated by nylon wool columns.
The technique of separating lymphocytes on nylon wool columns has been applied to sheep lymphocytes obtained from efferent lymph. The non-immunoglobulin-bearing (sIg-) lymphocytes which pass through the column were MLR-reactive, responsive only to T cell mitogens, and migrated in vivo like T lymphocytes described in rodents. Immunoglobulin-bearing (sIg+) lymphocytes were MLR-non-reactive, responsive to lipopolysaccharide and migrated in vivo like B lymphocytes in rodents. It is considered that the majority of sIg- lymphocytes obtained in this way are T lymphocytes and the majority of sIg+ lymphocytes are B lymphocytes.
D000818:Animals; D002448:Cell Adhesion; D002465:Cell Movement; D002469:Cell Separation / Q000379:methods; D005260:Female; D005374:Filtration; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D008934:Mitogens; D009757:Nylons; D011947:Receptors, Antigen, B-Cell / Q000032:analysis*; D012756:Sheep / Q000276:immunology*; D014373:Tuberculin
D008934:Mitogens; D009757:Nylons; D011947:Receptors, Antigen, B-Cell; D014373:Tuberculin