title: Antigenic and enzymatic architecture of Micrococcus lysodeikticus membranes established by crossed immunoelectrophoresis.
abstract: By crossed immunoelectrophoresis with membrane antiserum, 17 antigens have been detected in fractions from plasma membranes of M. lysodeikticus solubilized with Triton X-100. Absorption tests with protoplasts have demonstrated that eight of the antigens are expressed on the surface. Of these antigens the major one has been identified as a succinylated mannan. Five of the principal immunoprecipitates unaffected by absorption with protoplasts were shown by zymograms to possess the following enzymic activites: succinate dehydrogenase (EC 1.3.99.1), ATPase (EC 3.6.1.3), NADH dehyrogenase (EC 1.6.99.3)(two separate components), and malate dehydrogenase (EC 1.1.1.37). These enzymes or enzyme-complexes are, therefore, not expressed on the outer surface of the protoplast membrane.
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D000942:Antigens, Bacterial / Q000032:analysis*; D002462:Cell Membrane / Q000201:enzymology / Q000276:immunology; D008291:Malate Dehydrogenase / Q000378:metabolism; D008837:Micrococcus / Q000201:enzymology* / Q000276:immunology; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013499:Surface Properties
chemical_list: D000942:Antigens, Bacterial; D008291:Malate Dehydrogenase; D013385:Succinate Dehydrogenase; D009247:NADH, NADPH Oxidoreductases; D000251:Adenosine Triphosphatases