Studies of (Na+ + K+)-sensitive ATPase activity in pig lymphocytes. Effects of concanavalin A.
(Na+ + K+)-ATPase activity is demonstrated in plasma membranes from pig mesenteric lymph nodes. After dodecyl sulfate treatment plasma membranes have an 18-fold higher (Na+ + K+)-ATPase activity, while their ouabain-insensitive Mg2+-ATPase is markedly lowered. A solubilized (Na+ +K+)-ATPase fraction, obtained by Lubrol WX treatment of the membranes, has very high specific activity (21 mumol Pi/h per mg protein). Concanavalin A has no effect on these partially purified (Na+ + K+)-ATPase, while inhibits (40%) this activity in less purified fractions which still contain Mg2+-ATPase activity.
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D003208:Concanavalin A / Q000494:pharmacology*; D003902:Detergents / Q000494:pharmacology; D007700:Kinetics; D008214:Lymphocytes / Q000201:enzymology*; D011188:Potassium / Q000378:metabolism; D012964:Sodium / Q000378:metabolism
D003902:Detergents; D003208:Concanavalin A; D012964:Sodium; D000251:Adenosine Triphosphatases; D011188:Potassium