Studies on intestinal adenosine triphosphatases. I. Application of a semiautomated method to the rat intestinal brush borders.
Elaboration of a semiautomated kinetic test on LKB 8600 apparatus for ATPase is described, using the PK-LDH system. As optimal ionic conditions 3 mmol-1 - minus 1 potassium chloride and 100 mmol-1 - minus 1 sodium chloride are proposed for measurement of (Na+-K+)-ATPase activities of rat intestinal brush borders. NH+4 can substitute for K+. The coefficients of variation of the method are 2.4% for Mg2+-ATPase and 4.9% for (Na+-K+)-ATPase determinations.
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000641:Ammonia / Q000494:pharmacology; D000818:Animals; D001322:Autoanalysis; D004789:Enzyme Activation / Q000187:drug effects; D005069:Evaluation Studies as Topic; D007421:Intestine, Small / Q000201:enzymology*; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D008722:Methods; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D012964:Sodium / Q000494:pharmacology
D000641:Ammonia; D012964:Sodium; D000251:Adenosine Triphosphatases; D008274:Magnesium; D011188:Potassium