Activation of rabbit brain microsomal (Na+ plus K+)-dependent ATPase by a leukocytic product.
The properties of a (Na+ plus K+)-dependent ATPase (ATP phosphohydrolase, EC 3.6.1.3) activator contained in leukocytic extracts was investigated. Intact polymorphonuclear leukocytes release the activator in a time- and temperature-dependent process. It is non-dialyzable through cellophane; inactivated by protease, trypsin, or phenol; contains essential sulfhydryl groups; and is heat and acid labile. Treatment of ATPase with the activator and subsequent removable of the activator from mixtures did not reverse the ATPase activation.
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D001798:Blood Proteins / Q000378:metabolism*; D001921:Brain / Q000201:enzymology*; D004789:Enzyme Activation / Q000187:drug effects; D007962:Leukocytes / Q000378:metabolism*; D008861:Microsomes / Q000187:drug effects / Q000201:enzymology*; D011188:Potassium / Q000494:pharmacology; D011817:Rabbits; D012964:Sodium / Q000494:pharmacology; D014020:Tissue Extracts
D001798:Blood Proteins; D014020:Tissue Extracts; D012964:Sodium; D000251:Adenosine Triphosphatases; D011188:Potassium