Effects of exogenous dehydroepiandrosterone sulphate on various enzymes and on steroid metabolism in the guinea-pig.
Treatment of male guinea-pigs daily with an oral dose of 2 mg dehydroepiandrosterone (DHA) sulphate/100 g body weight for 2 weeks significantly reduced the glucose-6-phosphate dehydrogenase (G-6-PDH) activity of erythrocytes, liver, kidney and testis. Lactate dehydrogenase activity in plasma also decreased, but L-aspartate: 2-oxoglutarate aminotransferase (GOT) and L-alanine:2-oxoglutarate aminotransferase (GPT) activity in plasma remained unaffected. In liver and kidney, however, a significant rise in GOT and GPT was observed. A 2- to 3-7-fold increase of C19-steroids was observed in plasma, liver and kidney. In extracts of liver and kidney more than 60% of steroids were isolated from the sulphatide fraction. Only minor changes were detected in the metabolic pattern of C19-steroids, 17-hydroxysteroids prevailing in the free and sulphatide fractions, while 17-oxosteroids predominated in the sulphate and glucuronide fractions. A slight rise of cyclic AMP concentrations in liver and kidney tissue was attributed to the inhibition of phosphodiesterase by the DHA/G-6-PDH system
D015068:17-Ketosteroids / Q000378:metabolism; D000410:Alanine Transaminase / Q000032:analysis; D000734:Androstenediols / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000032:analysis; D003687:Dehydroepiandrosterone / Q000494:pharmacology*; D004912:Erythrocytes / Q000201:enzymology; D005954:Glucosephosphate Dehydrogenase / Q000097:blood*; D006168:Guinea Pigs; D007668:Kidney / Q000201:enzymology; D007770:L-Lactate Dehydrogenase / Q000097:blood*; D008099:Liver / Q000201:enzymology; D008297:Male; D008660:Metabolism / Q000187:drug effects; D013256:Steroids / Q000378:metabolism*; D013737:Testis / Q000201:enzymology
D015068:17-Ketosteroids; D000734:Androstenediols; D013256:Steroids; D003687:Dehydroepiandrosterone; D007770:L-Lactate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase