Purification and properties of adenosine triphosphatase solubilized from beef heart mitochondria by chloroform.
Soluble, oligomycin-insensitive ATPase released from beef heart mitchondria by chloroform extraction can be further purified by Sepharose 6B gel filtration. This purification increases enzyme activity 4--5 times (100-130U/mg). According to specific activity, high purity and ability to reconstitute oligomycin-sensitive complex, isolated ATPase is quite comparable with enzyme preparations isolated by other methods.
D000251:Adenosine Triphosphatases / Q000302:isolation & purification* / Q000378:metabolism; D000818:Animals; D002417:Cattle; D002725:Chloroform; D002847:Chromatography, Agarose; D066298:In Vitro Techniques; D008929:Mitochondria, Heart / Q000201:enzymology*; D009840:Oligomycins / Q000494:pharmacology; D012995:Solubility
D009840:Oligomycins; D002725:Chloroform; D000251:Adenosine Triphosphatases