[Diploid and aneuploid human cell lines from autopsy material in long term cultures. Investigations of growth behaviour and karyotype (author's transl)].
Experiences with the development and characterization of cell lines obtained from human autopsy material, mainly of peripartal deceased children with multiple malformations, are reported. On 7 diploid and 4 aneuploid fibroblast-like cell lines (2 cases of trisomy G, 2 cases of trisomy E) investigations of the whole life span in vitro, on the growth behaviour and morphology as well as karyotype analyses in the early, middle and late phases of cultivation were carried out. All cell lines showed a definite limited life span, their doubling potential being on the average greater than 30 +/- 5. Compared with results from literature, these values had been found to be between those of cells of embryonic and adult origin and are possibly dependent on the age of the donor. No morphologic signs or changes of cell kinetic parameters were found which might point to a spontaneous cell alteration. The karyotype determined in the early culture phase was stable until the natural end of the cell lines. The results may serve as an evidence for the stability of all investigated parameters and for a reproducible growth behaviour in diploid and aneuploid cell lines developed from human autopsy material, a prerequisite to sue them as an in vitro model for further investigations.
D000782:Aneuploidy; D001344:Autopsy; D002460:Cell Line; D002478:Cells, Cultured; D002902:Chromosomes, Human, 16-18; D004171:Diploidy; D004314:Down Syndrome / Q000235:genetics; D005347:Fibroblasts; D006128:Growth; D006801:Humans; D066298:In Vitro Techniques; D007231:Infant, Newborn; D007621:Karyotyping; D014314:Trisomy
Pathologischen Institut der Martin-Luther-Universität Halle-Wittenberg.