[Preparative isolation of protein III of the human erythrocyte membrane (author's transl)].
The paper describes a method for the large-scale isolation of protein III (protein E, major intrinsic protein) from the human erythrocyte membrane with little expenditure of time. By treatment of the erythrocyte ghosts with deluted HCl at pH 2.3 and 0 degrees C some membrane proteins can be extracted. From the remaining "rest" membranes, whose major constituents, besides phospholipides, are protein PAS-1, other carbohydrate containing proteins and protein III, the latter can be separated in pure form by means of gel filtration. The paper reports on the amount, purity and possible structural modifications of the protein III obtained by this isolation method.
D000596:Amino Acids / Q000032:analysis; D002845:Chromatography; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004910:Erythrocyte Membrane / Q000032:analysis*; D004912:Erythrocytes / Q000032:analysis*; D006801:Humans; D006851:Hydrochloric Acid; D006868:Hydrolysis; D007123:Immunoelectrophoresis, Two-Dimensional; D008565:Membrane Proteins / Q000032:analysis / Q000097:blood* / Q000302:isolation & purification; D012995:Solubility
D000596:Amino Acids; D008565:Membrane Proteins; D006851:Hydrochloric Acid
Präparative Isolierung des Protein III der meschlichen Erythrocytenmembran