[Interaction of platinum and palladium complexes with thiol groups of Ca2+-dependent ATPase from sarcoplasmic reticulum].
In native preparations of sarcoplasmic reticulum 10-12 thiol groups (in g-eqv per 10(5) g of protein) were estimated by the Benesh method (titration with AgNO3) and 2 thiol groups--by Ellman (titration with dithionitrobenzoic acid). After denaturation of the sarcoplasmic reticulum preparations with 8 M urea 10-12 thiol groups were also determined by the Ellman method. When the preparations were treated with platinum tetrachloride or with palladium diaminodichloride, only 3 thiol groups were estimated by the Benesh and no one - by the Ellman method. Platinum and palladium complexes inhibited also the Ca2+-dependent activity blocking the transport of Ca2+ in sarcoplasmic reticulum. The inhibition was partially removed by glutathione.
D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors*; D000818:Animals; D002118:Calcium; D066298:In Vitro Techniques; D010165:Palladium / Q000494:pharmacology*; D010984:Platinum / Q000494:pharmacology*; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000187:drug effects / Q000201:enzymology*
D010984:Platinum; D010165:Palladium; D000251:Adenosine Triphosphatases; D002118:Calcium
Vzaimodeĭstvie kompleksov platiny i palladiia s tiolovymi gruppami Ca2+-zavisimoĭ ATF-azy sarkoplazmaticheskogo retikuluma.