Further purification of the lymphocyte inhibiting extract from the thymus.
A crude thymic extract, demonstrated to possess both immunosuppressive activity on T lymphocytes in vivo, and inhibitory action on DNA synthesis in T lymphocytes in vitro, has been purified by ultra-filtration and ion exchange chromatography procedures. The immunosuppressive activity was found to be associated with a high molecular weight fraction (greater than 50,000 daltons), devoid of immunosuppressive properties, a basic peptide has been isolated which retains the in vitro inhibitory activity on DNA synthesis.
D000818:Animals; D002417:Cattle; D002852:Chromatography, Ion Exchange; D006131:Growth Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology; D008970:Molecular Weight; D012756:Sheep; D013950:Thymus Gland / Q000032:analysis*; D014462:Ultrafiltration
D006131:Growth Inhibitors