title: Interaction between proteoglycan subunit and type II collagen from bovine nasal cartilage, and the preferential binding of proteoglycan subunit to type I collagen.
abstract: We studied the interaction of proteoglycan subunit with both types I and II collagen. All three molecular species were isolated from the ox. Type II collagen, prepared from papain-digested bovine nasal cartilage, was characterized by gel electrophoresis, amino acid analysis and CM-cellulose chromatography. By comparison of type I collagen, prepared from papain-digested calf skin, with native calf skin acid-soluble tropocollagen, we concluded that the papain treatment left the collagen molecules intact. Interactions were carried out at 4 degrees C in 0.06 M-sodium acetate, pH 4.8, and the results were studied by two slightly different methods involving CM-cellulose chromatography and polyacrylamide-gel electrophoresis. It was demonstrated that proteoglycan subunit, from bovine nasal cartilage, bound to cartilage collagen. Competitive-interaction experiments showed that, in the presence of equal amounts of calf skin acid-soluble tropocollagen (type I) and bovine nasal cartilage collagen (type II), proteoglycan subunit bound preferentially to the type I collagen. We suggest from these results that, although not measured under physiological conditions, it is unlikely that the binding in vivo between type II collagen and proteoglycan is appreciably stronger than that between type I collagen and proteoglycan.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001667:Binding, Competitive; D002356:Cartilage / Q000378:metabolism*; D002417:Cattle; D003094:Collagen / Q000302:isolation & purification / Q000378:metabolism*; D006025:Glycosaminoglycans / Q000378:metabolism*; D008297:Male; D009666:Nose; D010206:Papain; D011509:Proteoglycans / Q000378:metabolism*; D012867:Skin; D014332:Tropocollagen / Q000378:metabolism
chemical_list: D000596:Amino Acids; D006025:Glycosaminoglycans; D011509:Proteoglycans; D014332:Tropocollagen; D003094:Collagen; D010206:Papain