title: Isolation of troponin-like complex from bovine brain cortex.
abstract: A troponin-like complex has been isolated from bovine brain cortex. This tropinin-like complex, with brain tropomyosin, confers Ca2+ sensitivity to the actin-activated myosin adenosinetriphosphatase (ATP phosphohydrolase, EC 3.6.1.3). That is, the Mg2+-stimulated ATPase activity generated by the interaction of purified muscle actin with muscle myosin is inhibited in the absence of Ca2+ but not in the presence of Ca2+ as a result of the addition of both brain tropomyosin and troponin-like complex. The troponin-like complex contains three components, one of which is similar in molecular weight to the troponin-T of skeletal actomyosin.
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D001923:Brain Chemistry; D002118:Calcium / Q000494:pharmacology; D002417:Cattle; D002540:Cerebral Cortex / Q000032:analysis* / Q000201:enzymology; D046911:Macromolecular Substances; D008274:Magnesium / Q000494:pharmacology; D008854:Microscopy, Electron; D008970:Molecular Weight; D009124:Muscle Proteins / Q000302:isolation & purification; D015879:Myosin Subfragments / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000302:isolation & purification / Q000378:metabolism; D011487:Protein Conformation; D014335:Tropomyosin / Q000302:isolation & purification; D014336:Troponin / Q000302:isolation & purification
chemical_list: D046911:Macromolecular Substances; D009124:Muscle Proteins; D015879:Myosin Subfragments; D009419:Nerve Tissue Proteins; D014335:Tropomyosin; D014336:Troponin; D000251:Adenosine Triphosphatases; D008274:Magnesium; D002118:Calcium