The effect of translation and transcription inhibitors on the synthesis of periplasmic phosphatases in E. coli.
Previous studies by others have indicated that the synthesis of secreted enzymes is unusually sensitive to many translation inhibitors and resistant, for about 30 min, to rifampicin. We have studied the sensitivity of secreted (periplasmic) phosphatases to such inhibitors. Alkaline phosphatase synthesis is more sensitive than total protein synthesis to tetracyclin and spectinomycin, but not to sparsomycin, streptomycin, chloramphenicol, kasugamycin, blasticidin S or thiostrepton; it is slightly more resistant than total protein synthesis to the latter two antibiotics. Acid hexose-phosphatase was also preferentially sensitive to tetracyclin and spectinomycin and also to kasugamycin. beta-galactosidase was also included in the study, as an intracellular enzyme, and was found to be preferentially inhibited ("repressed"), sometimes transiently, by all eight translation inhibitors. This effect did not seem to be mediated through cyclic AMP or guanosine tetraphosphate; the "repression" was still evident in mutants with altered rho factor indicating that it may also not be related to artificial polarity. Synthesis of both periplasmic phosphatases was immediately inhibited by rifampicin. These results differ from those found in previous studies with other organisms and suggest a reappraisal of the usual interpretation of these phenomena.
D000135:Acid Phosphatase / Q000378:metabolism*; D000469:Alkaline Phosphatase / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002701:Chloramphenicol / Q000494:pharmacology; D004926:Escherichia coli / Q000201:enzymology*; D006146:Guanidines / Q000494:pharmacology; D014176:Protein Biosynthesis / Q000187:drug effects*; D012293:Rifampin / Q000494:pharmacology; D013033:Sparsomycin / Q000494:pharmacology; D000198:Spectinomycin / Q000494:pharmacology; D013307:Streptomycin / Q000494:pharmacology; D013752:Tetracycline / Q000494:pharmacology; D013883:Thiostrepton / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects*
D000900:Anti-Bacterial Agents; D006146:Guanidines; D002701:Chloramphenicol; D013033:Sparsomycin; D000198:Spectinomycin; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D013752:Tetracycline; D013883:Thiostrepton; D012293:Rifampin; D013307:Streptomycin