Macrophage cytotoxicity in the mouse immune response against a skin allograft.
Macrophage-rich peritoneal cell populations from C57BL/6 mice grafted with DBA/2 skin were found to be cytotoxic against 51Cr-labeled target cells from the donor strain. Normal peritoneal macrophages were also rendered cytotoxic by incubation with acellular supernatants of mixed lymphocyte cultures (MLC) between an allograft recipient and a donor mouse. Supernatants alone were not cytotoxic. The macrophage arming factor(s) was found in supernatants when the MLC was performed after more than 6 to 9 days following grafting. In order to produce MAF, sensitized lymphocytes must usually be stimulated in a specific way by donor type cells. The armed macrophage cytotoxicity was, however, not found to be specific in these experiments.
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D002448:Cell Adhesion; D002860:Chromium Radioisotopes; D003601:Cytotoxicity Tests, Immunologic; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000276:immunology; D008223:Lymphoma / Q000276:immunology; D008264:Macrophages / Q000276:immunology*; D012515:Mast-Cell Sarcoma / Q000276:immunology; D051379:Mice; D008805:Mice, Inbred A; D008806:Mice, Inbred AKR; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D010537:Peritoneum / Q000166:cytology; D016038:Skin Transplantation; D013154:Spleen / Q000166:cytology; D013864:Thioglycolates; D014181:Transplantation Immunology; D014184:Transplantation, Homologous
D002860:Chromium Radioisotopes; D013864:Thioglycolates