Energy coupling to active transport in anaerobically grown mutants of Escherichia Coli K12.
1. Anaerobic uptake of proline requires either the presence of a coupled Mg2+-stimulated adenosine triphosphatase or anaerobic electron transport. 2. Anaerobic uptake of glutamine does not require anaerobic electron transport even in the absence of a coupled Mg+2-stimulated adenosine triphosphatase. 3. These results support previous suggestions [Berger (1973) Proc. Natl. Acad. Sci. U.S.A. 70, 1514--1518; Berger & Heppel (1974) J. Biol. Chem. 249, 7747-7755; Kobayashi, Kin & Anraku (1974) J. Biochem. (Tokyo) 76, 251-261] that two distinct mechanisms of energy coupling to active transport exist in Escherichia coli in that energization of anaerobic proline uptake requires the 'high-energy membrane state', whereas the energization of anaerobic glutamine uptake does not.
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000693:Anaerobiosis; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D004579:Electron Transport; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism*; D005650:Fumarates / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D005973:Glutamine / Q000378:metabolism; D008274:Magnesium / Q000494:pharmacology; D009154:Mutation; D009566:Nitrates / Q000494:pharmacology; D011392:Proline / Q000378:metabolism
D005650:Fumarates; D009566:Nitrates; D005973:Glutamine; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D011392:Proline; D000251:Adenosine Triphosphatases; D008274:Magnesium; D005947:Glucose