Gas chromatographic determination of unconjugated dehydroepiandrosterone, androstenedione, testosterone, pregnenolone and progesterone in human ovarian tissue.
A gas chromatographic method has been presented for the determination of unconjugated dehydroepiandrosterone, androstenedione, testosterone, pregnenolone and progesterone in human ovarian tissue. The procedure utilized radioactive tracers added to homogenate for correcting methodological loss, preliminary separation of steroids by thinlayer chromatography, acetylation, rechromatography on chromatoplate and gas chromatography on 3% SE-30 or 1% XE-60 columns with flame ionisation detection of steroids by using internal standards. Results of control experiments and representative clinical findings on normal and polycystic ovaries are reported.
D000728:Androgens / Q000032:analysis*; D000735:Androstenedione / Q000032:analysis; D002250:Carbon Radioisotopes; D002849:Chromatography, Gas; D003687:Dehydroepiandrosterone / Q000032:analysis; D005260:Female; D006801:Humans; D010053:Ovary / Q000032:analysis*; D011085:Polycystic Ovary Syndrome / Q000378:metabolism; D011374:Progesterone / Q000032:analysis; D011372:Progestins / Q000032:analysis*
D000728:Androgens; D002250:Carbon Radioisotopes; D011372:Progestins; D000735:Androstenedione; D003687:Dehydroepiandrosterone; D011374:Progesterone