Rat testis mitochondrial adenylate cyclase. Partial purification and characterization.
1. Adenylate cyclase (EC 4.6.1.1) from rat testis mitochondria has been solubilized by treatment with the non-ionic detergent Lubrol PX. The soluble enzyme was further purified by DEAE-cellulose chromatography. 2. The specific activity of the adenylate cyclase eluted from the DEAE-cellulose column was found to be four times higher than that of an intact mitochondrial preparation. At this step the enzyme shows a sedimentation coefficient of 4.2 S and a diffusion coefficient (D) of 3.12 - 10- minus 7 cm-2/sec. 3. Solubilization of the adenylate cyclase resulted in loss of responsiveness to gonadotrophic hormones. Addition of phosphatidylserine to the soluble preparation partially restored the activation of adenylate cyclase by human chorionic gonadotrophin. 4. The results of this study suggest that the activity of the adenylate cyclase may be dependent on the membrane-bound phospholipids and that the enzyme attached to the mitochondrial membranes has some properties which are similar to the adenylate cyclase found to be associated with other membrane systems of the cell.?
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000262:Adenylyl Cyclases / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D002848:Chromatography, DEAE-Cellulose; D003902:Detergents; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D007700:Kinetics; D008297:Male; D008928:Mitochondria / Q000201:enzymology*; D010718:Phosphatidylserines / Q000494:pharmacology; D051381:Rats; D012995:Solubility; D013737:Testis / Q000201:enzymology*
D006063:Chorionic Gonadotropin; D003902:Detergents; D010718:Phosphatidylserines; D000251:Adenosine Triphosphatases; D000262:Adenylyl Cyclases