Age-dependent change in the gel-electrophoretic pattern of proteoglycans of human growth cartilage.
The gel-electrophoretic pattern of dissociated proteoglycans was studied in 7 fetuses, 5 premature newborns, 4 term newborns, 5 infants and 5 children. The tibial growth cartilage was extracted with 4 M guanidinium chloride. After dialysis against 8 M urea at pH 7 the proteoglycans were obtained by ion chromatography in urea on DEAE cellulose and submitted to gel electrophoresis on polyacrylamide agarose gels. Gel electrophoresis of proteoglycans showed a different pattern in fetuses from that found in children. The change occurs in the first months of extrauterine life.
D000293:Adolescent; D000367:Age Factors; D002356:Cartilage / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005333:Fetus; D006025:Glycosaminoglycans / Q000378:metabolism*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D011509:Proteoglycans / Q000378:metabolism*
D006025:Glycosaminoglycans; D011509:Proteoglycans