[Use of culture media with sheep serum for maintenance and isolation of Leptospira strains].
A study was carried out on the effects of a culture medium prepared with sheep serum inactivated at 68 degrees C on Leptospira cultures. Good results were obtained showing that the medium with sheep serum can be used for the cultivation of Leptospira in view of the preparation of antigens for microscopic agglutination and complement fixation. In experiments on the isolation of germs from organocultures and haemocultures the proportion of positive results obtained with sheep serum was smaller than with the media containing rabbit serum.
D000818:Animals; D001769:Blood; D003470:Culture Media; D007919:Leptospira; D012756:Sheep
D003470:Culture Media
Folosirea mediilor de cultură cu ser de oaie pentru intreţinerea şi izolarea tulpinilor de leptospire