The binding constant of ATP to myosin S1 fragment.
The extent of ATP synthesis from ADP and Pi at the active centre of myosin subfragment 1 has been reinvestigated. The results have been interpreted using a treatment which is not dependent on the number or nature of the intermediates in the ATPase mechanism. An average value for the binding constant of ATP of (3.25 +/- 0.96) X 10(11) M-1 at pH 8.0 23 degrees C and ionic strength 0.12 M was obtained. Additional evidence is given to confirm that synthesis at the active site has been investigated.
D000244:Adenosine Diphosphate; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D007700:Kinetics; D046911:Macromolecular Substances; D008433:Mathematics; D009218:Myosins / Q000378:metabolism; D010710:Phosphates; D011485:Protein Binding; D011817:Rabbits
D046911:Macromolecular Substances; D010710:Phosphates; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; D009218:Myosins