Immunologically specific cytolytic activity induced in long-term mixed leukocyte culture cells by concanavalin A.
Stimulation of cells from long-term primary MLC with Con A resulted in the generation of CTL activity comparable in magnitude to that induced by reexposure of the cells to the original stimulating cellular antigen. CTL generated by stimulation of long-term MLC cells with ConA had lytic activity specific for the original stimulating alloantigen used in primary MLC. The pattern of stimulation of long-term MLC cells with Con A differed from that of restimulation with alloantigen in that there was no detectable CTL activity the first 24 hr after Con A stimulation and the peak lytic activity occurred later. Unlike restimulation with alloantigen early lytic activity after Con A stimulation was dependent on DNA synthesis. PHA also proved to be an effective agent for stimulating cytolytic activity in long-term MLC cells. The response to PHA was comparable in magnitude to that generated by Con A. Stimulation of long-term MLC cells with T cell mitogens gave decreased cell recoveries relative to restimulation with alloantigen, however, the lytic activity per cells recovered was generally greater in the mitogen-stimulated cultures.
D000818:Animals; D000961:Antilymphocyte Serum; D003208:Concanavalin A / Q000494:pharmacology*; D003601:Cytotoxicity Tests, Immunologic; D004247:DNA / Q000096:biosynthesis; D006649:Histocompatibility Antigens; D007111:Immunity, Cellular; D007156:Immunologic Memory; D007700:Kinetics; D037102:Lectins; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008757:Methylglucosides / Q000494:pharmacology; D051379:Mice; D008815:Mice, Inbred Strains; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
D000961:Antilymphocyte Serum; D006649:Histocompatibility Antigens; D037102:Lectins; D008757:Methylglucosides; D003208:Concanavalin A; D004247:DNA