Microsomal ATPase of kidney papilla: sensitivity to diuretics and other inhibitors.
Microsomal ATPase isolated from rat kidney papilla was compared to that from kidney medulla. Microsomal Na, K-ATPase from papilla was more sensitive to inhibition by ouabain, Cad N-ethyl-maleimide, Hg++, Thiomerin, ethacrynic acid and Furosemide than the enzyme from the medulla. Mg-ATPase of the papilla was less sensitive than the medullary enzyme to inhibition by Ca++, Cd++, N-ethyl-maleimide, Hg++, ethacrynic acid and Furosemide. Papillary Mg-ATPase was less sensitive than papillary Na, K-ATPase to all the inhibitors mentioned. Medullary Mg-ATPase was more sensitive than medullary Na, K-ATPase to inhibition by ethacrynic acid and Furosemide but less sensitive than medullary Na, K-ATPase to all the other inhibitors. Papillary Na, K-ATPase is the most sensitive to inhibition and papillary Mg-ATPase is the most resistant to inhibition by various diuretic drugs. The possible significance of these characteristics is discussed.
D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D004232:Diuretics / Q000494:pharmacology*; D004976:Ethacrynic Acid / Q000494:pharmacology; D005665:Furosemide / Q000494:pharmacology; D066298:In Vitro Techniques; D007668:Kidney / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D007679:Kidney Medulla / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D008297:Male; D008861:Microsomes / Q000187:drug effects / Q000201:enzymology*; D010042:Ouabain / Q000494:pharmacology; D051381:Rats; D013439:Sulfhydryl Reagents / Q000494:pharmacology
D004232:Diuretics; D013439:Sulfhydryl Reagents; D010042:Ouabain; D005665:Furosemide; D000251:Adenosine Triphosphatases; D004976:Ethacrynic Acid; D002118:Calcium