[ATPase activity in neuronal and glial enriched fractions of cerebral cortex in normal conditions and with hyperoxia].
The ATP-hydrolyzing activity was studied without addition of the exogenic cations and in the presence of Ca2+, Mg2+, Na+, K+ in myelin of rat brain cortex fractions enriched with neurons and glia. In the variant without addition of the exogenic cations the fraction enriched with neurons possesses the highest specific activity of ATPase and the myelin fraction possesses the lowest one. Addition of Ca2+, Mg2+, Na+, K+, triton X-100 activates ATPase in the nerve tissue cell fractions, ouabain inhibits the Mg2+, Na+, K+-ATPase activity. Action of oxygen under high pressure changes the percentage of transport ATPases distribution in neuropile and decreases the share of Na+, K+-ATPase sensitive to ouabain in myelin of the nerve tissue fractions enriched with neurons and glia.
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002540:Cerebral Cortex / Q000201:enzymology*; D008274:Magnesium / Q000494:pharmacology; D009457:Neuroglia / Q000201:enzymology; D009474:Neurons / Q000201:enzymology; D010042:Ouabain / Q000494:pharmacology; D010100:Oxygen / Q000494:pharmacology*; D011188:Potassium / Q000378:metabolism; D051381:Rats; D012964:Sodium / Q000378:metabolism
D010042:Ouabain; D012964:Sodium; D000251:Adenosine Triphosphatases; D008274:Magnesium; D011188:Potassium; D010100:Oxygen; D002118:Calcium
ATPaznaia aktivnost' neironal'noĭ i glial'noĭ obogashchennykh fraktsii kory golovnogo mozga v norme i pri gkperoksii.