title: Brain serotonin and estradiol retention in the hypothalamus and pituitary of the rat.
abstract: In order to investigate whether the capacity of hypothalamic and anterior pituitary tissue to concentrate and retain estradiol is affected by serotonin (5-HT), 3H-estradiol (3HE2) retention in these structures was measured after 5-HT synthesis inhibition by either parachlorophenylalanine (PCPA) or 6-fluoro-tryptophane (6 FTrp), or after destruction of midbrain raphe nuclei containing 5-HT cell bodies, as well as after administration of the 5-HT precursor 5-hydroxytryptophane (5-HTP). No modification in 3HE2 retention was observed after tryptophane hydroxylase inhibitors of raphe lesions; administration of the precursor only increased the steroid retention at very high, nonphysiological dose levels. It is concluded that the interaction of 5-HT with gonadotropic release cannot be accounted for by a direct effect on specific estrogenic receptors, but occurs at a different level of gonadotropic release regulating structures or directly on LH-RH neurons.
mesh_terms: D006916:5-Hydroxytryptophan / Q000008:administration & dosage / Q000494:pharmacology; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism; D002940:Circadian Rhythm; D003600:Cytosol / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D010134:Fenclonine / Q000494:pharmacology; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism*; D007276:Injections, Intraventricular; D008636:Mesencephalon / Q000502:physiology; D010902:Pituitary Gland / Q000378:metabolism*; D010903:Pituitary Gland, Anterior / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D012701:Serotonin / Q000378:metabolism*; D014364:Tryptophan / Q000031:analogs & derivatives
chemical_list: D012701:Serotonin; D004958:Estradiol; D014364:Tryptophan; D006916:5-Hydroxytryptophan; D010134:Fenclonine