Solubilization of bacterial membrane proteins using alkyl glucosides and dioctanoyl phosphatidylcholine.
The non-ionic detergent octyl glucoside solubilizes a substantial amount of Streptococcus faecalis membrane protein without loss of the monitored enzyme activities. A secondary detergent, dioctanoyl phophatidycholine, appears to increase the yield of solubilized material. In addition, the effect of ionic strength indicates that it may be possible to selectively extract groups of membrane proteins by their characteristic solubility at different ionic strengths. The solubilized membrane-associated enzymes, ATPase and NADH dehydrogenase, enter polyacrylamide gels as distict species. Electrophoretic studies suggest that there are two membrane-associated ATPase in the Streptococcus faecalis, one which dissociates from the membrane in the absence of Mg-2+ ions and the other which remains particulate until solubilized by detergents. Octyl glucoside can be easily removed from a solution containing solubilized proteins and lipid by dialysis.
D000251:Adenosine Triphosphatases / Q000032:analysis; D001426:Bacterial Proteins / Q000302:isolation & purification*; D002462:Cell Membrane / Q000201:enzymology; D003902:Detergents; D013293:Enterococcus faecalis / Q000032:analysis / Q000201:enzymology; D005960:Glucosides; D006027:Glycosides; D008722:Methods; D009247:NADH, NADPH Oxidoreductases / Q000032:analysis; D009994:Osmolar Concentration; D010713:Phosphatidylcholines; D011092:Polyethylene Glycols; D011485:Protein Binding; D012995:Solubility
D001426:Bacterial Proteins; D003902:Detergents; D005960:Glucosides; D006027:Glycosides; D010713:Phosphatidylcholines; D011092:Polyethylene Glycols; D009247:NADH, NADPH Oxidoreductases; D000251:Adenosine Triphosphatases