Molar concentrations of fibrinolytic components, especially free fibrinolysin, in vivo.
The levels of fibrinogen and of profibrinolysin (plasminogen) in urokinase-treated plasma as a function of time of incubation were measured. The profibrinolysin concentration was estimated through its complete conversion to fibrinolysin and the inhibition of the enzyme by crystalline soybean trypsin inhibitor. The dissociation constant of the FL-STI complex was determined to be 7 times 10-9 M. The average concentration of profibrinolysin in normal human citrated plasma was found to be 8 times 10-7 M. From the decrease of fibrinogen with time in the urokinase-treated plasma, the free fibrinolysin was calculated. Free fibrinolysin in normal human blood in vivo was estimated from the half-life of fibrinogen and other data obtained in this study to be present at a concentration of 1.7 times 10-10 M. The plasmakinase activity in vivo, expressed as urokinase molarity, is also about 2 times 10-10 M.
D005340:Fibrinogen / Q000032:analysis; D005341:Fibrinolysin / Q000032:analysis*; D005342:Fibrinolysis; D006801:Humans; D007700:Kinetics; D008722:Methods; D009391:Nephelometry and Turbidimetry; D009994:Osmolar Concentration; D010958:Plasminogen / Q000032:analysis; D013997:Time Factors; D014361:Trypsin Inhibitors; D014568:Urokinase-Type Plasminogen Activator / Q000494:pharmacology
D014361:Trypsin Inhibitors; D005340:Fibrinogen; D010958:Plasminogen; D005341:Fibrinolysin; D014568:Urokinase-Type Plasminogen Activator