Augmentation of human natural cell-mediated cytotoxicity by a soluble factor. I. Production of N-cell-activating factor (NAF).
Natural cell-mediated cytotoxicity (NCMC) like immune T cell-mediated cytotoxicity and antibody production is regulated by a soluble factor released during co-culture of lymphocytes with mitomycin C-treated lymphoblastoid cell lines. This N-cell-activating factor (NAF) enhances the activity of effector N cells and increases natural cytotoxicity. There appears to be no restriction for compatibility at the A and B locus of the major human histocompatibility complex in the production or activity of the factor. NAF was observed in the supernatant as soon as 2 days after initiation of mixed culture with a peak of production at 5 days. A soluble factor produced and released by T cells in response to stimulation by other cells acts by enhancing cytotoxicity of effector cells in NCMC, demonstrating a T-N cell cooperation.
D000818:Animals; D002460:Cell Line; D002469:Cell Separation; D002478:Cells, Cultured; D003470:Culture Media; D003602:Cytotoxicity, Immunologic; D006801:Humans; D007111:Immunity, Cellular; D007141:Immunoglobulin Fc Fragments; D007700:Kinetics; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes; D008222:Lymphokines / Q000096:biosynthesis*; D051379:Mice; D051381:Rats; D012397:Rosette Formation
D003470:Culture Media; D007141:Immunoglobulin Fc Fragments; D008222:Lymphokines