Energy transduction in photosynthetic bacteria. VIII. Activation of the energy-transducing ATPase by inorganic phosphate.
ATPase activity and ATP-induced energization of photosynthetic membranes from Rhodopseudomonas capsulata are stimulated by phosphate; the maximum stimulatory effect occurs at a concentration between 1 and 2 mM. The sensitivity of the ATPase to oligomycin increases in the presence of phosphate since all the Pi-stimulated activity is inhibited by this antibiotic. Aurovertin, which has no effect on ATPase in the absence of phosphate, inhibits completely the activity elicited by this anion. The addition of Pi induces a substantial increase in the V of ATPase activity without changing the affinity of the enzyme for ATP or ADP. Arsenate, at the same concentrations, produces effects very similar to those of phosphate. The stimulation by arsenate of the transfer of energy from ATP to the membrane suggests a non-hydrolytic role of this anion as a modifier of the ATPase activity.
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000963:Antimetabolites / Q000494:pharmacology; D001149:Arsenates / Q000494:pharmacology; D004735:Energy Transfer; D004789:Enzyme Activation / Q000187:drug effects; D006835:Hydrazones / Q000494:pharmacology; D007700:Kinetics; D008027:Light; D009570:Nitriles / Q000494:pharmacology; D009840:Oligomycins / Q000494:pharmacology; D010710:Phosphates / Q000494:pharmacology*; D010785:Photophosphorylation / Q000187:drug effects*; D012241:Rhodopseudomonas / Q000187:drug effects / Q000378:metabolism*
D000963:Antimetabolites; D001149:Arsenates; D006835:Hydrazones; D009570:Nitriles; D009840:Oligomycins; D010710:Phosphates; D000244:Adenosine Diphosphate; D000251:Adenosine Triphosphatases