Adenosine triphosphatase activity associated with purified cholinergic synaptic vesicles of Torpedo marmorata.
A rapid method for purifying Torpedo electric organ vesicles is described, which employs an isoosmotic continuous sucrose-glycine gradient followed by chromagography on CPG-10-3000 porous glass beads. The synaptic vesicles have a buoyant density of 1.057 g/ml. The purified vesicles are free of cholinesterase, lactate dehydrogenase and Na+, K+-stimulated ATPase activity. They contain a  ouabaininsensitive, Na+, K+-inhibited, Mg2+, Ca2+-stimulated ATPase activity. This is further stimulated by acetylcholine but not by choline.
D000109:Acetylcholine / Q000494:pharmacology; D000251:Adenosine Triphosphatases / Q000302:isolation & purification / Q000378:metabolism*; D000818:Animals; D002794:Choline / Q000494:pharmacology; D004557:Electric Organ / Q000201:enzymology*; D005399:Fishes / Q000378:metabolism*; D066298:In Vitro Techniques; D010042:Ouabain / Q000494:pharmacology; D010275:Parasympathetic Nervous System / Q000201:enzymology / Q000648:ultrastructure*; D011188:Potassium / Q000494:pharmacology; D012964:Sodium / Q000494:pharmacology; D013572:Synaptic Vesicles / Q000201:enzymology*
D010042:Ouabain; D012964:Sodium; D000251:Adenosine Triphosphatases; D002794:Choline; D000109:Acetylcholine; D011188:Potassium