Incorporation of T4 phage DNA into a specific DNA fraction from the higher plant Matthiola incana.
Isolated T4 phage DNA (sigma=1.694 g/ml) is applied to seedlings of the crucifer Matthiola incana (DNA density sigma=1.698 g/ml). The phage DNA can partly be reextracted from the plants in a specific DNA fraction, which is predominantly characterized by its unusual high density (high density complex=HDC; sigma=1.724 g/ml). DNA:DNA hybridization studies show that phage specific DNA sequences are preserved in the HDC. Results of BrdUrd labeling of the plant DNA before and during incubation with T4DNA suggest that the HDC is composed of T4DNA and a plant DNA component of high density. The analysis of ultrasonicated HDC confirms this suggestion. The ability of plant cells to recognize and handle T4 DNA specifically is discussed.
D003090:Coliphages; D004247:DNA / Q000096:biosynthesis*; D004279:DNA, Viral / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D010944:Plants / Q000378:metabolism*; D014170:Transformation, Genetic
D004279:DNA, Viral; D004247:DNA