Purification of carcinoembryonic antigen by removal of contaminating mucopolysaccharides.
The fraction of carcinoembryonic antigen preparations not bound by concanavalin A was studied. A significant portion of this nonbound fraction of low antigenic activity was shown to be mucopolysaccharides by gas chromatography-mass spectrometry, cellulose acetate strip electrophoresis, and depolymerization by bovine testicular hyaluronidase.
D002272:Carcinoembryonic Antigen / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas; D003110:Colonic Neoplasms / Q000276:immunology; D003208:Concanavalin A; D005965:Glucuronates / Q000032:analysis; D006025:Glycosaminoglycans / Q000302:isolation & purification*; D006801:Humans; D008113:Liver Neoplasms / Q000276:immunology; D009362:Neoplasm Metastasis
D002272:Carcinoembryonic Antigen; D005965:Glucuronates; D006025:Glycosaminoglycans; D003208:Concanavalin A