[Effect of an erythrocytic chalone on the electrophoretic mobility of bone marrow cells in mice].
Five fractions were isolated with the aid of cell electrophoresis in the phycol density gradient from the bone marrow of mice treated with physiological saline (control) and with erythrocytic chalone (experiment). The absolute and the relative distribution of cells in control and experimental fractions differed: the maximal number of cells in control (up to 78%) was revealed in fractions 3--5, and the minimal (up to 22%)--in fractions 1--2. 25 minutes after the action of erythrocytic cheilon there occurred redistribution of the cell material in all the fractions. The number of cells increased 2.6-fold in fraction 1. Analysis of smear "myelograms" from fraction 1 in experiment and control demonstrated that the formed element count in fraction 1 increased on account of the erythrocytic series cells. Among the proliferating erythron cells reduction of electrophoretic mobility proved to be the greatest in proerythroblasts. It is supposed that as a result of interaction between the erythrocytic cheilon and the receptors of the membranes of the proliferating erythron cells there occurred a reduction of their electrical change and changes in the intracellular processes leading to delay of mitoses.
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects / Q000502:physiology; D001854:Bone Marrow Cells; D002455:Cell Division / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D004586:Electrophoresis; D004912:Erythrocytes; D005362:Ficoll; D006131:Growth Inhibitors / Q000494:pharmacology*; D051379:Mice; D051381:Rats; D013997:Time Factors
D006131:Growth Inhibitors; D005362:Ficoll
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