title: Selective medium for isolation of Treponema hyodysenteriae.
abstract: Pure cultures of six pathogenic isolates of Treponema hyodysenteriae, the colonic mucosal scrapings of seven pigs with acute swine dysentery, and feces from seven unaffected pigs were diluted in phosphate-buffered saline and plated on Trypticase soy agar with 5% citrated bovine blood (TSA) and TSA with various levels of spectinomycin (TSA-S). The plates were incubated at 42 degrees C in a vented GasPak jar with a cold palladium catalyst and either 80:20 H2-CO2 by evacuation and refilling or a H2-CO2 generator envelope. Viable cell counts of the six pathogenic isolates were not altered by plating on TSA-S with 400 mug of spectinomycin per ml (TSA-S400) as compared with TSA alone. Dilutions of colonic mucosal scrapings from seven pigs with acute swine dysentery showed numbers of T. hyodysenteriae to be unchanged when plated on TSA-S400. Flora other than T. hyodysenteriae present in acute swine dysentery was inhibited, on the average, by 99.99%. Plating of dilutions of feces of unaffected pigs on TSA-S400 showed inhibition of flora that averaged more than 99.9%. Pathogenicity of T. hyodysenteriae was not altered by isolation or serial passage on TSA-S400.
mesh_terms: D000818:Animals; D003106:Colon; D003470:Culture Media; D004403:Dysentery / Q000382:microbiology / Q000662:veterinary; D005069:Evaluation Studies as Topic; D005243:Feces / Q000382:microbiology; D007413:Intestinal Mucosa / Q000382:microbiology; D000198:Spectinomycin; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology; D014208:Treponema / Q000302:isolation & purification* / Q000472:pathogenicity; D014211:Treponemal Infections / Q000382:microbiology / Q000662:veterinary
chemical_list: D003470:Culture Media; D000198:Spectinomycin