[Metabolism of myocardial poly A+-containing mRNA normally and in compensatory cardiac hyperfunction].
Two fractions of mRNA--polyA+ and polyA- containing mRNA--were found in rat heart muscle by affinity chromatography using polyU cellulose. These fractions constituted 30% and 70% of total mRNA, respectively. The ratio of polyA+/polyA- mRNA was not altered in myocardium under heart hyperfunction and in physiological ageing. Duration of life of polyA+ containing mRNA was 4 hrs in normal heart. A; the beginning of myocardium hyperfunction the period of mRNA life was decreased down to 2-3 hrs; this pattern of mRNA life alteration did not differ from control one in prolonged heart hypertrophy within 6 months. The rate of polyA+ containing mRNA synthesis was increased by 70% at the early steps of heart hyperfunction as compared with normal state; it decreased below the normal state in long-term hypertrophy of myocardium. In development of heart hyperfunction acceleration of polyA+ containing mRNA synthesis was more distinct than an increase in rRNA synthesis.
D000818:Animals; D001012:Aorta, Abdominal; D001017:Aortic Coarctation / Q000378:metabolism; D006332:Cardiomegaly / Q000378:metabolism*; D002846:Chromatography, Affinity; D007700:Kinetics; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D011061:Poly A / Q000032:analysis / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D013997:Time Factors
D012333:RNA, Messenger; D011061:Poly A
Metabolizm poli-A+-soderzhashcheĭ iRNK miokarda v norme i pri kompensatornoĭ giperfunktsii serdtsa.