Immunosuppressive activity of heterologous antimacrophage sera.
Antisera against rat macrophages were raised in rabbits and tested individually, serologically and in vivo, by injecting 1.5 ml/100 g into LEW rats 1 and 4 days following a graft of CDF rat skin. 1. Erythrocyte absorption reduced the mean lymphocytotoxic titer of antimacrophage sera from 1:16 to 1:1, but yielded strongly immunosuppressive sera. They extended the graft survival time from the normal rate of 11-14 days up to 217 days. 2. Additional absorption with lymphocytes reduced this activity significantly, but still yielded very active sera (graft survival up to 83 days). 3. After the procedure for serologically exhaustive absorption was repeated, an in vivo control for exhaustive absorption of anti-lymphocyte antibodies was performed. The results showed that the double-absorbed sera did not exhibit reduced immunosuppressive activity. 4. The above activity was found in 9/16 antisera. Seven antisera were inactive in vivo, though they showed similar in vitro activity with respect to their toxic titers against macrophages. These results confirm and extend our earlier functional and morphological studies on AMS. They are discussed in relationship to the controversial data from literature.
D000042:Absorption; D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D004912:Erythrocytes / Q000276:immunology; D007106:Immune Sera; D007111:Immunity, Cellular; D007165:Immunosuppression Therapy; D008214:Lymphocytes / Q000276:immunology; D008264:Macrophages / Q000276:immunology*; D011817:Rabbits / Q000276:immunology; D051381:Rats; D016038:Skin Transplantation; D014181:Transplantation Immunology; D014184:Transplantation, Homologous
D007106:Immune Sera