Liver and immune responses. IV. Characteristics of the liver cell-lymphocyte interaction.
Rat liver cells prepared by collagenase treatment and separated by density gradient centrifugation with Ficoll-Hypaque solution (specific gravity 1.120), containing only hepatocytes (80%) and Kupffer cells (20%), were found to stimulate strongly Ag-B-incompatible lumphocytes without 2-mercaptoethanol in culture. The same liver cells could also stimulate Ag-B-compatible lymphocytes in the presence of 2-mercaptoethanol. A positive response was only seen when the liver cell numbers were 1 approximately 2% of the responding lymphocytes. Viable liver cells inhibited the reaction in a two-way culture.
D000818:Animals; D002478:Cells, Cultured; D006649:Histocompatibility Antigens / Q000032:analysis; D007728:Kupffer Cells / Q000276:immunology; D007958:Leukocyte Count; D008099:Liver / Q000166:cytology / Q000276:immunology*; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains / Q000276:immunology
D006649:Histocompatibility Antigens