Isolation and localization from chicken gizzard of an inhibitory protein for Mg2+-activated skeletal muscle actomyosin ATPase.
An inhibitory protein for Mg2+-activated actomyosin ATPase from rabbit skeletal muscle was prepared from frozen chicken gizzard and purified by DEAE-Sephadex chromatography and gel filtration. 2. The inhibition by this protein was released by the addition of skeletal muscle troponin C and was independent of gizzard tropomyosin. 3. Localization of the inhibitory protein in gizzard muscle tissue and gizzard thin filaments was demonstrated by immunohistological techniques and immunodiffusion tests.
D000205:Actomyosin / Q000378:metabolism*; D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D002645:Chickens; D004789:Enzyme Activation; D005895:Gizzard, Avian / Q000502:physiology*; D005779:Immunodiffusion; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology*; D009132:Muscles / Q000201:enzymology*; D009210:Myofibrils / Q000502:physiology; D011506:Proteins / Q000494:pharmacology*; D011817:Rabbits; D014336:Troponin / Q000378:metabolism
D011506:Proteins; D014336:Troponin; D000205:Actomyosin; D000251:Adenosine Triphosphatases; D008274:Magnesium