The use of aryl azido ATP analogs as photoaffinity labels for myosin ATPase.
The synthesis of ATP analogs containing a photoactive aryl azido grouping coupled to the 3' hydroxyl of ATP is described. The potential effectiveness of these analogs in the investigation of nucleotide-binding regions is outlined and this effectiveness demonstrated by their photodependent inhibition of subfragment 1 ATPase. The use of 14C-labeled azido ATP demonstrates an almost stoichiometric covalent binding of the analog. Because of their potential application to other systems, a number of reactions describing the reactivity of the 3' hydroxyl of the nucleotide ribose are outlined in an Appendix.
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000378:metabolism; D000345:Affinity Labels; D001386:Azides; D001665:Binding Sites; D007700:Kinetics; D009218:Myosins / Q000378:metabolism*; D010782:Photolysis; D013113:Spin Labels / Q000138:chemical synthesis; D013329:Structure-Activity Relationship
D000345:Affinity Labels; D001386:Azides; D013113:Spin Labels; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases; D009218:Myosins