The determination of clioquinol in biological materials by extractive alkylation and gas-liquid chromatography.
Clioquinol, or 5-chloro-7-iodo-8-hydroxyquinoline (Vioform), and the internal standard, 5,7-dichloro-8-hydroxyquinoline are extracted from biological material in the form of their tetrahexylammonium salts into dichloromethane, where, in the presence of a methylating agent, both clioquinol and the standard are spontaneously transformed into their O-methyl derivatives. These derivatives can be purified by base-specific extraction and subsequently determined by gas chromatography; concentrations down to 10 ng per sample may be assayed. The method is compared with a previously reported procedure based on the O-acetyl derivatives.
D000273:Adipose Tissue / Q000032:analysis; D000478:Alkylation; D000818:Animals; D002849:Chromatography, Gas; D002853:Chromatography, Liquid; D007464:Clioquinol / Q000032:analysis* / Q000097:blood / Q000652:urine; D006801:Humans; D008722:Methods; D008745:Methylation; D013552:Swine
D007464:Clioquinol