Factors affecting the stimulating capacity of mitogen-transformed, glutaraldehyde-treated mouse spleen cells in the mixed lymphocyte reaction.
Mouse spleen cells transformed by certain mitogens and subsequently fixed with glutaraldehyde can be utilized as stimulators in the mixed lymphocyte reaction. In addition to bacterial lipopolysaccharide which has been shown previously to be effective, we have found the other B cell mitogens, dextran sulfate and Concanavalin A-Sepharose, to be functional. The ability of the treated cells to activate allogeneic cells in MLR depends upon the concentration of glutaraldehyde and the type of tissue culture medium utilized.
D000447:Aldehydes / Q000494:pharmacology*; D000818:Animals; D003208:Concanavalin A / Q000494:pharmacology; D003470:Culture Media; D003911:Dextrans / Q000494:pharmacology; D005260:Female; D005976:Glutaral / Q000494:pharmacology*; D066298:In Vitro Techniques; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008934:Mitogens / Q000494:pharmacology*; D013154:Spleen / Q000276:immunology*
D000447:Aldehydes; D003470:Culture Media; D003911:Dextrans; D008070:Lipopolysaccharides; D008934:Mitogens; D003208:Concanavalin A; D005976:Glutaral