[Influence of detergents and inhibition of Na+,K+-ATPase and Mg2+ATPase on erythrocyte membrane SH-groups].
The article deals with the influence of sodium dodensylsulfate, lubrol and also of inhibition of Na+,K+-ATPase and Mg2+-ATPase activities by N-ethyl malemid on the form of curves of erythrocyte membranes amperometric titration by Ag+. It is shown that changes in a conformational structure of proteins membranes under the influence of detergents results in the transformation of SH-group of type I to SH-groups of type II. A 70% fall of Na+,K+-ATPase activity has the same result. So, the form of the curves for titration of erythrocyte membranes sulphydryl groups may serve as an indicator to the morpholocial and functional state of the membranes protein part.
D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors*; D003902:Detergents / Q000494:pharmacology*; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000201:enzymology*; D004912:Erythrocytes / Q000201:enzymology*; D005033:Ethylmaleimide / Q000494:pharmacology*; D006801:Humans; D008274:Magnesium / Q000378:metabolism; D011092:Polyethylene Glycols / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism; D011487:Protein Conformation; D012964:Sodium / Q000378:metabolism; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013438:Sulfhydryl Compounds
D003902:Detergents; D013438:Sulfhydryl Compounds; D012967:Sodium Dodecyl Sulfate; D011092:Polyethylene Glycols; D012964:Sodium; D000251:Adenosine Triphosphatases; D008274:Magnesium; D005033:Ethylmaleimide; D011188:Potassium
Vliianie detepgentov i ingibirovaniia Na+, K+-ATPazy i Mg2+-ATP-azy na NA SH-gruppy membrane eritrotsitov.