F1-ATPase-catalyzed synthesis of ATP from oleoylphosphate and ADP.
Purified preparations of F1-ATPase (ATP phosphohydrolase; EC 3.6.1.3) isolated from yeast mitochondria catalyze the reaction of oleoylphosphate with ADP to yield ATP and oleic acid. Formation of ATP is specifically inhibited by the F1-ATPase inhibitor 1799 and by dinitrophenol. In the presence of F1, dinitrophenol "uncouples" the synthase reaction by causing rapid hydrolysis of oleoylphosphate without ATP formation. It is proposed that this F1 catalyzed ATP synthesis reaction corresponds to the terminal chemical step in oxidative phosphorylation.
D000244:Adenosine Diphosphate / Q000378:metabolism*; D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D008928:Mitochondria / Q000201:enzymology; D009829:Oleic Acids; D009840:Oligomycins / Q000494:pharmacology; D009943:Organophosphorus Compounds / Q000378:metabolism; D010085:Oxidative Phosphorylation / Q000187:drug effects; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000648:ultrastructure; D013997:Time Factors
D009829:Oleic Acids; D009840:Oligomycins; D009943:Organophosphorus Compounds; D000244:Adenosine Diphosphate; C016022:oleoylphosphate; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases