title: Regulation of phosphate metabolism in Neurospora crassa: identification of the structural gene for repressible alkaline phosphatase.
abstract: Five additional mutants of Neurospora crassa have been isolated that lack the repressible alkaline phosphatase. The mutations in these strains map at a previously assigned locus on Linkage Group V designated pho-2 (GLEASON and METZENBERG 1974). The five new mutants, as well as three previously isolated by GLEASON and METZENBERG (1974), were examined for the presence of cross-reacting material to antibody prepared against purified wild-type enzyme. Two of the mutants produced high levels of cross-reacting material, thus providing evidence that the pho-2 locus includes the structural gene for the repressible alkaline phosphatase. Two revertants were obtained from one of the mutants that contained cross-reacting material. Neither revertant produced an enzyme that could be distinguished physicochemically from that of wild type. A method for measuring very low levels of repressible alkaline phosphatase in crude extracts is also described.
mesh_terms: D000469:Alkaline Phosphatase / Q000276:immunology / Q000378:metabolism*; D000906:Antibodies; D002874:Chromosome Mapping; D003429:Cross Reactions; D004794:Enzyme Repression; D005796:Genes; D009154:Mutation; D009491:Neurospora / Q000201:enzymology*; D009492:Neurospora crassa / Q000201:enzymology*
chemical_list: D000906:Antibodies; D000469:Alkaline Phosphatase