Effects of streptozotocin added in vitro on insulin biosynthesis and release in isolated rat islets.
The effect of different concentrations of streptozotocin was tested in vitro on isolated rat islets. The following parameters of islet-cell activity were measured: 1) incorporation of 3H-leucine into proinsulin and into total islet protein; 2) IRI release into the medium, and 3) islet proinsulin content. It appears from the preliminary results shown here that both insulin release and biosynthesis were decreased by streptozotocin in a way which was dose- and time-dependent.
D000818:Animals; D066298:In Vitro Techniques; D007328:Insulin / Q000096:biosynthesis / Q000378:metabolism*; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D007930:Leucine / Q000378:metabolism; D008297:Male; D011384:Proinsulin / Q000096:biosynthesis; D051381:Rats; D013311:Streptozocin / Q000494:pharmacology*
D007328:Insulin; D013311:Streptozocin; D011384:Proinsulin; D007930:Leucine