title: Effect of cyclophosphamide on the immune response to sheep erythrocytes in the mouse. II. A study of enzyme activities in the draining lymph node.
abstract: Young adult CFW mice, injected with sheep erythrocytes, were treated with cyclophosphamide and sacrificed at different times. The activity of acid phosphatase and for non-specific esterase was measured by semi-quantitative methods in smears prepared from cell suspension of draining lymph node. In cyclophosphamide-treated mice was noted a significant decrease in acid phosphatase activity in lymphoid cells, expressed both as average activity in single lymphoid cell and a decrease in proportion of cells exhibiting enzyme activity. Also a short-term decrease was observed in activity of non-specific esterase caused both by disappearance of highly active large lymphoid and blast cells as well as decrease in percentage of enzyme-positive small lymphocytes.
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism*; D000818:Animals; D003520:Cyclophosphamide / Q000494:pharmacology*; D004912:Erythrocytes / Q000276:immunology*; D004950:Esterases / Q000378:metabolism*; D007114:Immunization; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000201:enzymology / Q000276:immunology*; D051379:Mice; D013997:Time Factors
chemical_list: D003520:Cyclophosphamide; D004950:Esterases; D000135:Acid Phosphatase