Fractionated precipitation of acid macropolyanions by dialysis, a simple method for the estimation of DNA in complex biological samples.
After efficient extraction by para-aminosalicylate, chopping, grinding and eventual sonication, the macropolyanions are transformed into their cetyltrimethylammonium salts. These have differing solubilities, strongly depending on ionic strength. The cationic detergent-macropolyanionic salts are solubilized by high salt concentration. Salt is then dialysed out, rendering the polyanions highly insoluble in a sequential fashion. The insolubilized components are determined quantitatively by monitoring turbidity, which in case of DNA is strictly proportionate to its concentration. This relation is not affected by other components. This makes DNA determination possible even in crude aqueous extracts. The method has been applied to different objects, such as bacteria, plants, animals, soil and activated sludge. The method may prove to be especially useful in research of environmental poisons e.g. in rivers, lakes or clarifiers.
D000818:Animals; D000838:Anions; D002593:Cetrimonium Compounds; D011232:Chemical Precipitation; D004247:DNA / Q000032:analysis*; D003956:Dialysis; D008722:Methods; D009994:Osmolar Concentration; D012441:Saccharomyces cerevisiae / Q000032:analysis; D012987:Soil / Q000032:analysis; D014337:Trout / Q000378:metabolism
D000838:Anions; D002593:Cetrimonium Compounds; D012987:Soil; D004247:DNA