Effect of 2-mercaptoethanol on the mixed leukocyte reaction in man. Probable effect on blastogenic factor stabilization.
When 2-mercaptoethanol (2-ME) is added to human mixed leukocyte cultures at the beginning of the culture period, only a very weak effect is seen. When 2-ME is added after a few days' culture, a marked enhancement of cellular transformation occurs. 2-ME can also enhance the effect of blastogenic factor (BF) containing media and it is possible that this effect depends on a stabilization or protection of BF preventing the break-down of BF in cellular systems.
D003470:Culture Media; D006801:Humans; D007962:Leukocytes / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects*; D007959:Lymphocyte Culture Test, Mixed; D008623:Mercaptoethanol / Q000494:pharmacology*; D008937:Mitomycins / Q000494:pharmacology; D013997:Time Factors
D003470:Culture Media; D008937:Mitomycins; D008623:Mercaptoethanol