title: The effects of 3-methylindole on hemolysis, transport of Na+, and ATPase activities of bovine erythrocytes.
abstract: Biochemical effects of 3MI on cellular membranes were investigated. This study was conducted to examine the effects of 3MI on the hemolysis of erythrocytes, the transport of 22Na+ in resealed erythrocyte ghosts, and on the ATPase activities of erythrocyte membranes. The percent of hemolysis as a function of 3MI incubation time was sigmoidal. Seventy-five percent of the hemoglobin was released with the second 2 hr of incubation during which the concentration of 3MI in the cells reached a plateau of 2500 mug/ml of packed RBC. The effect of 3MI at a subhemolytic concentration on passive and active 22Na+ transport were not significant. The total and Mg2+-dependent ATPase activities in the membranes were significantly increased after 1 hr of incubation with 3MI at concentrations of 100, 200, 300, 400 and 500 mug/ml (P less than or equal to 0/ml (P smaller than or equal to 0.02).
mesh_terms: D000251:Adenosine Triphosphatases / Q000097:blood*; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D001693:Biological Transport, Active / Q000187:drug effects; D002417:Cattle; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology; D004912:Erythrocytes / Q000276:immunology / Q000378:metabolism; D006461:Hemolysis / Q000187:drug effects*; D007211:Indoles / Q000494:pharmacology*; D008274:Magnesium / Q000378:metabolism; D011188:Potassium / Q000378:metabolism; D012862:Skatole / Q000008:administration & dosage / Q000494:pharmacology*; D012964:Sodium / Q000378:metabolism*; D013395:Sucrose; D013997:Time Factors
chemical_list: D007211:Indoles; D013395:Sucrose; D012964:Sodium; D012862:Skatole; D000251:Adenosine Triphosphatases; D008274:Magnesium; D011188:Potassium