[Purification of rat brain succinate-semialdehyde dehydrogenase and study of its inhibition by branched chain fatty acids].
Rat brain succinic semialdehyde deshydrogenase has been purified 1300 fold. This enzyme is inhibited non competitively by the same branched chain fatty acids which inhibit GABA-transaminase competitively with respect to GABA. The respective activities of GABA-T and SSADH found in rat brain indicate that at anticonvulsant doses, the acids dipropylacetic and 2-methyl 2-ethyl caproic preferentially inhibit GABA-transaminase thus inducing a rise in cerebral GABA level. This increase is therefore not due to metabolism of the succinic semialdehyde by GABA-T.
D000612:4-Aminobutyrate Transaminase / Q000037:antagonists & inhibitors; D000445:Aldehyde Oxidoreductases / Q000037:antagonists & inhibitors* / Q000302:isolation & purification; D000818:Animals; D001921:Brain / Q000201:enzymology*; D005227:Fatty Acids / Q000494:pharmacology*; D007700:Kinetics; D051381:Rats; D013329:Structure-Activity Relationship
D005227:Fatty Acids; D000445:Aldehyde Oxidoreductases; D000612:4-Aminobutyrate Transaminase
Purification de la semi aldéhyde succinique déshydrogénase de cerveau de rat et étude de son inhibition par des acides gras ramifiés