title: The biosynthesis of the mitochondrial ADP, ATP translocator.
abstract: The biosynthesis of the ADP,ATP carrier was studied in mitochondria of Neurospora crassa. The carrier was isolated as the carboxyatractylate-protein complex and characterized in dodecylsulphate/polyacrylamide gel electrophoresis to have a Mr = 33 000. Applying the inhibitors chloramphenicol for the intramitochondrial translation and cycloheximide for extramitochondrial translation, the site of synthesis of this polypeptide was found to be extramitochondrially located.
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism*; D001692:Biological Transport; D002352:Carrier Proteins / Q000302:isolation & purification; D002701:Chloramphenicol / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D008928:Mitochondria / Q000378:metabolism*; D000226:Mitochondrial ADP, ATP Translocases / Q000096:biosynthesis*; D008970:Molecular Weight; D009491:Neurospora / Q000378:metabolism*; D009492:Neurospora crassa / Q000378:metabolism*; D009713:Nucleotidyltransferases / Q000096:biosynthesis*; D014176:Protein Biosynthesis / Q000187:drug effects
chemical_list: D002352:Carrier Proteins; D000244:Adenosine Diphosphate; D002701:Chloramphenicol; D000255:Adenosine Triphosphate; D000226:Mitochondrial ADP, ATP Translocases; D003513:Cycloheximide; D009713:Nucleotidyltransferases