Unsaturated fatty acid mutants of Neurospora crassa.
Unsaturated fatty acid (ufa) auxotrophs of Neurospora crassa were obtained by treatment of conidia with N-methyl-N'-nitro-N-nitrosoguanidine followed by isolation on media containing polyunsaturated fatty acids suspended in Tergitol NP-40. The 24 mutants for which reisolates were obtained from crosses with wild type were assigned to two complementation classes, ufa-1 and ufa-2, located on linkage group V. Unsaturated fatty acids with varying degrees of unsaturation, chain length, and double-bond position as well as different steric configurations were tested for growth requirements.
D003433:Crosses, Genetic; D003470:Culture Media; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D005816:Genetic Complementation Test; D008040:Genetic Linkage; D009154:Mutation; D009491:Neurospora / Q000378:metabolism*; D009492:Neurospora crassa / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*
D003470:Culture Media; D005231:Fatty Acids, Unsaturated