Effects of free fatty acids on fibrinolytic activity.
A novel method for the estimation of fibrinolytic activity is proposed. In this method, a fibrin clot suspension is used as a substrate (fibrin is known to be a physiological substrate of plasmin). The fibrin clot suspension was prepared by homogenization of human fibrin clots. With this method, we found that free fatty acids inhibited the plasmin activity, and long-chain, unsaturated free fatty acids had a particularly strong inhibitory action on plasmin. As regards the mechanism of the inhibitory action, free fatty acids may not inhibit complex formation between plasmin and fibirin, but may make it impossible for plasmin to act on fibrin due to deformation of the surface of the fibrin clot.
D005230:Fatty Acids, Nonesterified / Q000494:pharmacology*; D005337:Fibrin; D005341:Fibrinolysin / Q000378:metabolism; D005342:Fibrinolysis / Q000187:drug effects*; D006801:Humans; D007700:Kinetics; D009829:Oleic Acids / Q000494:pharmacology; D013329:Structure-Activity Relationship
D005230:Fatty Acids, Nonesterified; D009829:Oleic Acids; D005337:Fibrin; D005341:Fibrinolysin