New mixed lymphocyte microculture test harvested by a simple device developed for this purpose.
The methodology of the conventional mixed lymphocyte culture test has been simplified and miniaturized by developing a micromethod employing Terasaki trays with a final culture volume of 20 mul containing between 10,000 and 20,000 responding and stimulating cells each/culture. This represents one-tenth the cell concentration of the conventional mixed lymphocyte culture. For this new test, a special microharvester has been designed and developed. The test allows accurate and reproducible results giving a high level of discrimination between the different levels of response.
D002478:Cells, Cultured; D006801:Humans; D007106:Immune Sera; D037102:Lectins; D007959:Lymphocyte Culture Test, Mixed / Q000295:instrumentation / Q000379:methods*; D008214:Lymphocytes / Q000276:immunology / Q000528:radiation effects; D011830:Radiation Effects
D007106:Immune Sera; D037102:Lectins