Histochemical localization of adenosine triphosphatase and thiamine pyrophosphatase in the digestive system of a teleost fish, Ophiocephalus punctatus.
The localization of ATPase and thiamine pyrophosphatase in the digestive system of Ophiocephalus punctatus has been studied. In stomach ATPase is found in the free border of the mucosa, gastric glands, submucosal connective tissue nuclei and muscularis. Thiamine pyrophosphatase is localized only in the mucosa and gastric gland cells. In the intestine, pyloric caeca and rectum, ATPase is distributed along the brush border of the columnar epithelial cells, their nuclei and cytoplasm. Mild activity is also found in the nuclei of submucosa and muscularis. The activity is stronger in the intestine than in the other portions. Thiamine pyrophosphatase activity in these portions is restircted only to the goblet cells. In the liver ATPase activity is associated both with the cytoplasm and nucleus of the hepatic cells. Thiamine pyrophosphatase activity is maximum in the centro-lobular portion.
D000251:Adenosine Triphosphatases / Q000032:analysis*; D000818:Animals; D002454:Cell Differentiation; D002470:Cell Survival; D004064:Digestive System / Q000201:enzymology*; D005247:Feeding Behavior; D005399:Fishes; D006651:Histocytochemistry; D007422:Intestines / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D009061:Mouth Mucosa / Q000166:cytology; D011755:Pyrophosphatases / Q000032:analysis*; D013270:Stomach / Q000201:enzymology; D013834:Thiamine Pyrophosphatase / Q000032:analysis*
D000251:Adenosine Triphosphatases; D011755:Pyrophosphatases; D013834:Thiamine Pyrophosphatase