[Standardization of in-vitro methods for diagnosing allergic disorders].
The various "in vitro" techniques were studied and the conclusion was reached that the hemagglutination and complement fixation tests were the most suitable for diagnosis. Sera were selected from 377 patients with very positive intracutaneous reactions to the antigens in question, the distribution being as follows: 110 sera positive to milk, 114 sera positive to eggs, 95 sera with a very marked intracutaneous reaction with gramineous pollen, and 58 with house dust. By means of the hemagglutionation technique of Boyden and the complement fixation test of Kolmer with these antigens, it was determined that the diagnostic reliability for food substances was 94,4% in the sera with positive anamnesis. For milk, there was 100% reliability in bronchial asthma and atopic dermatitis. With respect to pollen, hemagglutination gave 100% reliability in patients with positive anamnesis. The percentage with complement fixation was somewhat lower. Comparing the diagnostic reliability with hemagglutination, house dust gave an arithmetic average of 82,6% in positive anamnesis. With these antigens, hemagglutination by Boyden's technique appears the most suitable method.
D000818:Animals; D001249:Asthma / Q000175:diagnosis; D003168:Complement Fixation Tests; D003876:Dermatitis, Atopic / Q000175:diagnosis; D004391:Dust; D004531:Eggs / Q000009:adverse effects; D005512:Food Hypersensitivity / Q000175:diagnosis; D005767:Gastrointestinal Diseases / Q000175:diagnosis; D006386:Hemagglutination Tests; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis*; D066298:In Vitro Techniques; D008892:Milk / Q000276:immunology; D011058:Pollen; D012220:Rhinitis / Q000175:diagnosis; D006255:Rhinitis, Allergic, Seasonal / Q000175:diagnosis; D014581:Urticaria / Q000175:diagnosis
D004391:Dust
Zur Standardisierung von In-vitro-Methoden bei der Diagnostik allergischer Krankheiten