Fate of input oncornavirion RNA--biological studies.
The fate of input Friend leukemia virus RNA was studied using labeled input virus. The appearance of nuclear RNA-DNA hybrid molecules and the apparent integration of input virion RNA with host cell DNA was studied using a series of inhibitors of DNA or protein synthesis, cell growth conditions, and an intercalating agent. Under all these conditions of infection, little to no viral-specific RNA-DNA hybrid molecules were formed. These data demonstrate that the formation of such RNA-DNA hybrid structures requires conditions of infection that allow provirus synthesis and integration. Furthermore, they suggest that at least a fraction of input virion RNA may transiently become integrated with host cell DNA.
D000327:Adsorption; D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology; D003561:Cytarabine / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism; D004996:Ethidium / Q000494:pharmacology; D005467:Floxuridine / Q000494:pharmacology; D005622:Friend murine leukemia virus / Q000254:growth & development / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000378:metabolism*; D014764:Viral Proteins / Q000096:biosynthesis; D014779:Virus Replication
D004279:DNA, Viral; D012367:RNA, Viral; D014764:Viral Proteins; D005467:Floxuridine; D003561:Cytarabine; D004247:DNA; D003513:Cycloheximide; D004996:Ethidium