The effect of halides (NaCl and NaI) on in vitro pancreatic elastase activity.
The effect of I- and C1- on the enzymatic degradation of elastin pancreatic elastase has been studied. At low halide concentrations both ions show a marked stimulation of elastolysis, I- being significantly more effective than Cl-. Kinetic plots of enzyme activity indicate that anions enhance the susceptibility of the substrate to enzymatic and non-enzymatic degradation. If the halide concentration is raised inhibition of elastolysis occurs starting at 30 mM NaCl and 300 mM NaI. This inhibitory effect is explained by a direct attack of the halides leading to increasing destabilization and inactivation of the enzyme.
D004305:Dose-Response Relationship, Drug; D004549:Elastin / Q000378:metabolism; D007454:Iodides / Q000494:pharmacology*; D007700:Kinetics; D010179:Pancreas / Q000201:enzymology; D010196:Pancreatic Elastase / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology*; D012974:Sodium Iodide / Q000494:pharmacology*
D007454:Iodides; D012965:Sodium Chloride; D004549:Elastin; D010196:Pancreatic Elastase; D012974:Sodium Iodide