[Localization of mitochondrial cytochrome b using specific antibodies].
Specific antibody has been obtained against cytochrome b (pig heart mitochondria). It inhibits the electron transport of the respiratory chain in the intact mitochondria at the cytochrome b site of the inner mitochondrial membrane. It has no effect on the isolated submitochondrial particles which are inside-out inner membrane vescicles free of any outer membrane or outside-out inner membrane. These findings indicate a probably not transmembranous topologic localization of cytochrome b; this component of the respiratory chain seems located near the outer side of the inner mitochondrial membrane.
D000818:Animals; D000906:Antibodies; D003580:Cytochromes / Q000032:analysis* / Q000276:immunology; D004579:Electron Transport; D008566:Membranes / Q000201:enzymology; D008929:Mitochondria, Heart / Q000201:enzymology*; D013367:Submitochondrial Particles / Q000201:enzymology; D013552:Swine
D000906:Antibodies; D003580:Cytochromes
Localisation mitochondriale du cytochrmoe b à l'aide d'anticorps spécifiques.