[The mode of action of antifungal agents. Effect of horse erythrocyte membranes on amphotericin B].
The Tris-HCl solution of the heptaen antibiotic, amphotericin B, gives a very important circular dichroism exhibiting a disymetric couplet. The absorption is affected by a blue shift of the maxima. The addition of erythrocyte membranes reduces the couplet and increases the small negative peaks to positive values at 422 and 399 nm. As a time function the evolution of spectra is hyperbol like. Such transformations are more important than those obtained with molecular cholesterol. These striking modifications of the C. D. spectra are correlated with conformational changes of the aggregative form of the antibiotic.
D000666:Amphotericin B / Q000378:metabolism; D001665:Binding Sites; D002784:Cholesterol / Q000378:metabolism; D002942:Circular Dichroism; D004910:Erythrocyte Membrane / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D008563:Membrane Lipids / Q000378:metabolism; D008968:Molecular Conformation; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature
D008563:Membrane Lipids; D000666:Amphotericin B; D002784:Cholesterol
Etude du mode d'action des antifongiques. Effet des membranes d'érythrocytes de cheval sur l'amphotéricine B