title: Immunochemical identity of the high and low molecular weight forms of Galapagos marine iguana hemoglobin.
abstract: 1. Two forms of Galapagos marine iguana methemoglobin, with molecular weights of 140,000 and 70,000 daltons, were identified in iguana RBC lysates by Sephadex G-200 molecular sieve fractionation. 2. The 140,000 dalton ferric hemoglobin was isolated by DEAE-Sephadex A-50 ion-exchange chromatography and found to be pure by electrophoretic and immunological criteria. 3. Immunochemical analyses revealed the high and low molecular weight hemoglobins to be antigenically identical.
mesh_terms: D000818:Animals; D006454:Hemoglobins / Q000276:immunology / Q000302:isolation & purification*; D007076:Iguanas / Q000097:blood*; D007106:Immune Sera; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D008116:Lizards / Q000097:blood*; D008970:Molecular Weight
chemical_list: D006454:Hemoglobins; D007106:Immune Sera