Metabolism of nitrosamines in vivo IV. Isolation of 3-hydroxy-1-nitrosopyrrolidine from rat urine after application of 1-nitrosopyrrolidine.
After i. p. application of 6 mg/30 muCi/kg 2,5- and 3,4--14C-nitrosopyrrolidine about 20% of the radioactivity is exhaled as 14-CO2 and about 7% is found in the urine. One of the urinary metabolites was identified by thin layer chromatography, combined GC/MS spectrometry and ultraviolet absorption as 3-hydroxy-1-nitrosopyrrolidine.
D000818:Animals; D002250:Carbon Radioisotopes; D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas; D005243:Feces / Q000032:analysis; D006900:Hydroxylation; D008297:Male; D013058:Mass Spectrometry; D009602:Nitrosamines / Q000378:metabolism* / Q000652:urine; D009696:Nucleic Acids / Q000378:metabolism; D011759:Pyrrolidines / Q000378:metabolism*; D051381:Rats; D013056:Spectrophotometry, Ultraviolet
D002250:Carbon Radioisotopes; D009602:Nitrosamines; D009696:Nucleic Acids; D011759:Pyrrolidines