Cell-mediated and humoral immune responses in mice. I. Necessary conditions for the detection of delayed-type hypersensitivity.
Footpad assay of delayed-type hypersensitivity to bovine serum albumin (BSA) was studied. Injection of saline solution of BSA (S-BSA) into footpads of sensitized mice resulted in the development of only the local Arthus-type reaction. Injection of alumprecipitated BSA (AP-BSA), however, was effective in generating both Arthus- and delayed-type reactions. The difference between S-BSA and AP-BSA in the ability of elicitation of delayed-type reaction may be attributable to the difference in the retention of these two forms of antigen in the footpad, since S-BSA was found to be eliminated much more rapidly from footpads than AP-BSA.
D000818:Animals; D000941:Antigens / Q000008:administration & dosage; D001183:Arthus Reaction / Q000276:immunology / Q000473:pathology; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D006968:Hypersensitivity, Delayed / Q000276:immunology* / Q000473:pathology; D007111:Immunity, Cellular; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA / Q000276:immunology*; D012710:Serum Albumin, Bovine / Q000276:immunology / Q000378:metabolism
D000941:Antigens; D012710:Serum Albumin, Bovine