[Influence of immobilized ribonuclease on the cell-free protein synthesis in the wheat system (author's transl)].
In order to increase the efficiency of different mRNAs from wheat seedlings in carrying out cell-free protein synthesis in the wheat system, efforts were made to remove endogenous mRNA. In this direction, we checked the possibility of using immobilized RNase. Treatment of the cell-free system or its components with this enzyme caused a large decrease in the efficiency of poly tu-directed incorporation of labeled amino acids. This effect did not coincide with an equivalent degradation of RNA, as has been shown by analysis of ribosomes and polysomes. The results are discussed in the light of recent findings of some authors.
D002474:Cell-Free System; D007930:Leucine / Q000378:metabolism; D010649:Phenylalanine / Q000378:metabolism; D010940:Plant Proteins / Q000096:biosynthesis*; D011072:Poly U; D011132:Polyribosomes / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000302:isolation & purification* / Q000378:metabolism; D012260:Ribonucleases; D012270:Ribosomes / Q000378:metabolism; D014908:Triticum
D010940:Plant Proteins; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D011072:Poly U; D010649:Phenylalanine; D012260:Ribonucleases; D007930:Leucine
Einfluss trägergebundener Ribonuclease auf die zellfreie tproteinsynthese im Weizensystem