Antibody to Physarum myosin. I. Preparation and functional effects.
Preparation of antibody to Physarum myosin is described, and evidence is presented that the antibody is specific for this molecule. A diffusion coefficient of 1 X 10(-7) cm2/s is estimated. The antibody interfered with myosin enzyme activity and with superprecipitation of actomyosin. It did not cross-react with rabbit striated muscle myosin.
D000205:Actomyosin; D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors; D000818:Animals; D000908:Antibodies, Fungal / Q000302:isolation & purification*; D011232:Chemical Precipitation; D005779:Immunodiffusion; D009132:Muscles / Q000276:immunology; D009218:Myosins / Q000276:immunology*; D009235:Myxomycetes / Q000276:immunology*; D010804:Physarum / Q000276:immunology*; D011817:Rabbits
D000908:Antibodies, Fungal; D000205:Actomyosin; D000251:Adenosine Triphosphatases; D009218:Myosins