Structure and metabolism of glycoproteins and glycosaminoglycans secreted by organ cultures of rabbit trachea.
1. When cultured in medium 199 in an atmosphere of O2+CO2 (95:5) rabbit tracheal explants retained their viability for up to 21 days. 2. The explants secreted into the culture medium three electrophoretically separable components which were eluted in the non-retarded fraction from Sephadex G-200. 3. Digestion with papain and fractionation with a LiCl gradient on DEAE-cellulose resulted in the separation of these components, which were identified as a sialic acid-rich glycoprotein of epithelial origin, and chondroitin sulphate and hyaluronic acid from sub-epithelial cartilage and connective tissue. 4. Incorporation of radioactive precursors (  D-[U-14-C]glucose, D-[1-14-C]glucosamine, D-[1-14-C]galactose and Na2-35SO4) into these secreted macromolecules was indicative of their active biosynthesis by the tracheal tissue.
D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism; D002807:Chondroitin / Q000032:analysis; D004586:Electrophoresis; D005260:Female; D005690:Galactose / Q000378:metabolism; D005944:Glucosamine / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006023:Glycoproteins / Q000096:biosynthesis / Q000378:metabolism; D006025:Glycosaminoglycans / Q000096:biosynthesis / Q000378:metabolism; D006820:Hyaluronic Acid / Q000032:analysis; D008297:Male; D009924:Organ Culture Techniques; D010101:Oxygen Consumption; D011817:Rabbits; D012794:Sialic Acids / Q000032:analysis; D013431:Sulfates / Q000378:metabolism; D014132:Trachea / Q000378:metabolism*
D006023:Glycoproteins; D006025:Glycosaminoglycans; D012794:Sialic Acids; D013431:Sulfates; D002245:Carbon Dioxide; D006820:Hyaluronic Acid; D002807:Chondroitin; D005947:Glucose; D005944:Glucosamine; D005690:Galactose