[The influence of mode and intensity of homogenization on the absolute value and stability of oxygen consumption of guinea pig liver homogenates (author's transl)].
The influence of five different methods of homogenisation (1. The method according to Potter and Elvehjem, 2. A modification of this method called Potter S, 3. The method of Dounce, 4. Homogenisation by hypersonic waves and 5. Coarce-grained homogenisation with the "Mikrofleischwolf") on the absolute value and stability of oxygen uptake of guinea pig liver homogenates has been investigated in simultaneous measurements. All homogenates showed a characteristic fall of oxygen uptake during measuring time (3 hours). The modified method according to Potter and Elvehjem called Potter S showed reproducible results without any influence by homogenisation intensity.
D000818:Animals; D001671:Biochemistry / Q000379:methods; D006168:Guinea Pigs; D008099:Liver / Q000378:metabolism*; D010101:Oxygen Consumption
Die Bedeutung der Homogenisationsart und -intensität für die Grösse und Konstanz der Sauerstoffaufnahme von Meerschweinchen-Leberhomogenat.