[Effect of novoimanine on the cellular permeability indices of staphylococci].
Novoimanine is an antibacterial drug from Hypericum perforatum L. When used in the bacteriostatic concentration, i.e. 0.5 gamma/ml, it induced release of potassium ions from the cells of Staphylococcus aureus 209P and had no effect on release of the UV-absorbing compounds and 14C-amino acids. In addition, incubation of the cells with novoimanine (2.5--50 gamma/ml) provided "preservation" in them of the earlier absorbed 14C-amino acids, while in the control cells their level decreased. In a concentration of 100 gamma/ml novoimanine stimulated activity of ATP-ase and alkaline phosphatase by 34 and 37-57 per cent respectively. Histones F1 and F3 of the calf thymus induced an intensive release of 14C-amino acids from the cells of staphylococci and increased the activity of ATP-ase by 6-10 times. The data of the study suggested that the effect of novoimanine on the cytoplasmic membrane was limited and different from that on the polycationic antibacterial agents.
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001692:Biological Transport / Q000187:drug effects; D002463:Cell Membrane Permeability / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation / Q000187:drug effects; D006657:Histones / Q000494:pharmacology; D010936:Plant Extracts; D011188:Potassium / Q000378:metabolism; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000378:metabolism
D000596:Amino Acids; D000900:Anti-Bacterial Agents; D006657:Histones; D010936:Plant Extracts; D000469:Alkaline Phosphatase; D000251:Adenosine Triphosphatases; D011188:Potassium
Vliianie novoimanina na nekotorye pokazateli kletochnoĭ pronitsaemosti stafilokokkov.