Necrotaxis: a scanning and transmission electron microscopy study.
This paper describes an easy method to determine a "necrotactic rosette forming index (NRI) of leucocytes" after destruction of a red cell by a laser beam. Among the drugs tested, only cytochalasin B (0.5 mug/ml) is found to significantly lower NRI. The lectins PHA and CON-A did not have any effect on the NRI. Patients with infections are found to have increased NRI. Transmission and scanning electron microscopy have permitted the study of fine structurale details of the entire phenomenon.
D000818:Animals; D001001:Anura; D002449:Cell Aggregation / Q000187:drug effects; D002464:Cell Migration Inhibition; D002633:Chemotaxis / Q000187:drug effects*; D003208:Concanavalin A / Q000494:pharmacology; D003571:Cytochalasin B / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000528:radiation effects / Q000648:ultrastructure; D006801:Humans; D007104:Immune Adherence Reaction; D007834:Lasers; D037102:Lectins / Q000494:pharmacology; D007962:Leukocytes / Q000502:physiology*; D008854:Microscopy, Electron / Q000379:methods; D008855:Microscopy, Electron, Scanning / Q000379:methods; D008858:Microscopy, Phase-Contrast; D009504:Neutrophils / Q000648:ultrastructure; D010587:Phagocytosis
D037102:Lectins; D003208:Concanavalin A; D003571:Cytochalasin B