title: Enzyme histochemical studies of the preserved rat liver.
abstract: The effect of depersolone, of the oxygenation of the perfusing solution and of phenoxybenzamine pretreatment has been studied in the isolated rat liver intermittently perfused with a solution containing low molecular weight dextran at 4 degrees C. The use in liver preservation of Collins' C3-solution and of a special albumin-containing solution was tested. The behaviour of acid and alkaline phosphatase, esterase, lactate dehydrogenase and adenosine triphosphatase in the preserved liver was followed by means of histochemical methods allowing semi-quantitative evaluation. Pretreatment with phenoxybenzamine and perfusion by an albumin-containing solution reduced the lesion of the liver, while prednisolone and oxygenation of the perfusion solution improved preserving effect only moderately.
mesh_terms: D000135:Acid Phosphatase / Q000032:analysis; D000251:Adenosine Triphosphatases / Q000032:analysis; D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D004950:Esterases / Q000032:analysis; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008099:Liver / Q000201:enzymology*; D009926:Organ Preservation; D010101:Oxygen Consumption; D010643:Phenoxybenzamine; D011239:Prednisolone; D051381:Rats; D014021:Tissue Preservation
chemical_list: D010643:Phenoxybenzamine; D011239:Prednisolone; D007770:L-Lactate Dehydrogenase; D004950:Esterases; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D000251:Adenosine Triphosphatases