An improved assay of UV-induced thymine-containing dimers in Saccharomyces cerevisiae.
The method for assaying thymine-containing dimers in yeast is based on highly efficient ([3H]-deoxythymidine-5'-monophosphate) DNA-specific labelling and employs ascending thin layer chromatography. It allows satisfactory quantitative analysis down to UV-doses of 500 erg/mm2.
D002855:Chromatography, Thin Layer; D004260:DNA Repair; D011830:Radiation Effects; D012441:Saccharomyces cerevisiae / Q000378:metabolism* / Q000528:radiation effects; D013942:Thymine Nucleotides / Q000378:metabolism*; D014466:Ultraviolet Rays
D013942:Thymine Nucleotides