Inhibition of axoneme and dynein ATPase from sea urchin sperm by free ATP.
Sea urchin sperm flagellar ATPase (EC 3.6.1.3) has magnesium-ATP as an effective substrate and is inhibited by free ATP. The inhibition is prevented by high concentration of KCl or NaCl. 0.4 M KCl extracts 48% of ATPase activity from axoneme. The 0.4 M KCl extract and 0.4 M KCl-treated axoneme are also inhibited by free ATP and this inhibition is reversed by KCl. Dynein purified twice by sucrose density gradient centrifugation is also inhibited by free ATP; this inhibition is also reversed by KCl.
D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors*; D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D005407:Flagella / Q000201:enzymology; D007700:Kinetics; D008297:Male; D011189:Potassium Chloride / Q000494:pharmacology; D012617:Sea Urchins; D013094:Spermatozoa / Q000201:enzymology*
D011189:Potassium Chloride; D000255:Adenosine Triphosphate; D000251:Adenosine Triphosphatases