Proteoglycans of soluble fraction of mouse mastocytoma.
Proteoglycans have been isolated from a high speed supernatant fraction of a mouse mastocytoma by procedures which should minimize alteration of the native protein-polysaccharide molecule. The methods used include in vivo labeling proteoglycans with 35S-sulfate, 3H-leucine and 3H-lysine, centrifugation of the tumor homogenate at 105,000 g, cetylpyridinium fractionation of the supernatant, and further purification of some of the fractions obtained by DEAE-cellulose column chromatography, gel filtration on Sepharose 4B and cellulose acetate electrophoresis. Two major sulfated proteoglycans were obtained, one containing keratan sulfate-like material (KSP-S), the other a heparin-like polymer (HP-S). The presence in HP-S of a compound similar to heparin was confirmed by its digestibility with flavobacterium heparinase. HP-S contained about 4 per cent protein. Glycine was the predominant amino acid, and serine did not appear to be involved in the peptide-carbohydrate linkage. The proteoglycan present in HP-S appeared to be homogeneous when examined using cellulose acetate electrophoresis. KSP-S was found to contain sialic acid and its protein content was significantly higher than that of HP-S. Glutamic and aspartic acids were the most abundant amino acids in KSP-S.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D005643:Fucose / Q000032:analysis; D005688:Galactosamine / Q000032:analysis; D005944:Glucosamine / Q000032:analysis; D006025:Glycosaminoglycans / Q000032:analysis*; D006601:Hexoses / Q000032:analysis; D012515:Mast-Cell Sarcoma / Q000032:analysis*; D051379:Mice; D009374:Neoplasms, Experimental / Q000032:analysis; D011509:Proteoglycans / Q000032:analysis*; D012794:Sialic Acids / Q000032:analysis; D013464:Sulfuric Acids / Q000032:analysis; D014574:Uronic Acids / Q000032:analysis
D000596:Amino Acids; D006025:Glycosaminoglycans; D006601:Hexoses; D011509:Proteoglycans; D012794:Sialic Acids; D013464:Sulfuric Acids; D014574:Uronic Acids; D005643:Fucose; D005688:Galactosamine; D005944:Glucosamine