[Macrophage depletion test in guinea pigs].
In the present investigations heterologous lymphokine-containing fluids were examined in albino guinea pigs for their macrophage depletion activity. Cell-free ascites from Ehrlich ascites tumor and leukemia L 1210 of mice as well as supernatants of PHA-stimulated peripheral blood lymphocyte cultures were used. Ascites of Ehrlich's ascitic tumor and Leukemia L 1210 led to a significant depletion of macrophages. The application of supernatants of PHA-stimulated human lymphocyte cultures resulted in a dose-dependent reduction of peritoneal macrophages. On the other hand, a stimulation of the number of peritoneal macrophages by control supernatants of non-stimulated lymphocyte cultures was observed. The findings indicate the existence of two lymphokines having opposite effects.
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology* / Q000187:drug effects; D002286:Carcinoma, Ehrlich Tumor / Q000503:physiopathology; D002452:Cell Count; D002478:Cells, Cultured; D003470:Culture Media; D006168:Guinea Pigs; D037102:Lectins / Q000494:pharmacology; D007939:Leukemia L1210 / Q000503:physiopathology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects; D008222:Lymphokines / Q000494:pharmacology*; D008264:Macrophages
D003470:Culture Media; D037102:Lectins; D008222:Lymphokines
Untersuchungen zum Makrophagenschwundtest beim Meerschweinchen