Response of hematopoietic cell lines derived from patients with Down's syndrome and from normal individuals to mitomycin C and caffeine.
Eight human hematopoietic cell lines, five derived from blood of patients with Down's syndrome (DS) and three from normal persons, were treated with mitomycin C (MC) and caffeine at various dose levels and for various durations. An increased rate of chromosome aberrations was found in all treated cultures. The extent of aberrations was correlated with the dose levels and duration; no difference occurred in sensitivity between the group of DS and normal lines at all dose levels and for all durations. Studies on the effect of MC severely reduced cell viability, but no difference was found in the rate of reduction of viable cell counts between DS and normal lines. Inhibition of mitoses by MC in DS lines, however, seemed stronger than that in normal lines.
D002110:Caffeine / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000187:drug effects*; D004314:Down Syndrome / Q000235:genetics* / Q000473:pathology; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D006801:Humans; D008937:Mitomycins / Q000494:pharmacology*
D008937:Mitomycins; D002110:Caffeine