[Immunocytological study of the gonadotrophic and thyrotrophic cells of the rat adenohypophysis].
On paraffin or semi-thin sections various anti-LH or anti-TSH sera stain indifferently all the thyrotrophs and the gonadotrophs. Inversely anti-beta-TSH, anti-beta-LH or anti-beta-FSH purified sera permit the discrimination of these two cell populations. The constancy of fixation of the anti-beta-LH and anti-beta-FSH sera on all the gonadotrophs gives evidence of their ability to produce both FSH and LH. However in a few female rats the central gonadotrophs are stained more weakly by anti-beta-FSH serum than by anti-beta-LH serum. The purification of the antisera by adjunction of hormonal antigens (alpha subunits or heterologous hormone) does not enable, with the PAP technique on thin sections, a selective staining of the secretory granules of the thyrotrophs or of the gonadotrophs.
D000818:Animals; D000918:Antibody Specificity; D005260:Female; D005640:Follicle Stimulating Hormone / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D007986:Luteinizing Hormone / Q000378:metabolism*; D008297:Male; D010903:Pituitary Gland, Anterior / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D013972:Thyrotropin / Q000378:metabolism*
D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D013972:Thyrotropin
Etude cytoimmunologique des cellules gonadotropes et des cellules thyréotropes de l'adénohypophyse du rat.