Influence of adrenalectomy, castration, and light cycle on steroid hydroxylase activity in the adult male rat liver.
The contribution of the adrenals, the testes, and a light/dark cycle to the regulation of hepatic steroid hydroxylases was studied in vitro by measuring transformation rates (nmol/mg protein/min) of dehydroepiandrosterone (DHA). Normal and adrenalectomized male rats were kept in 12 h light:12 h dark (12L:12D) or in constant light (CL) for 1 week. Normal and castrated rats were kept in 12L:12D for 3 weeks. Rats were killed at 4-h intervals over a 36-h period, hepatic microsomal fractions were incubated with [4-14C]DHA, and C-7 and C-16-oxygenated products were quantitated. The overall mean rate of 7alpha-hydroxylation decreased in normal rats exposed to CL. Rates of both 7alpha- and 16alpha-hydroxylation were significantly greater in normal rats than in the respective group of treated rats, except for the 7alpha-hydroxylase in castrated rats. These results indicate that 16alpha-hydroxylase activity is both gonadal- and adrenal-dependent, whereas 7alpha-hydroxylase activity is adrenal-dependent only. Both enzymes are sensitive to light/dark modulations, although a circadian rhythm could not be conclusively established.
D000311:Adrenal Glands / Q000502:physiology*; D000315:Adrenalectomy; D000633:Aminopyrine N-Demethylase / Q000378:metabolism; D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases; D002369:Castration; D002940:Circadian Rhythm; D000072467:Cytochrome P450 Family 2; D003687:Dehydroepiandrosterone / Q000378:metabolism; D008027:Light; D008099:Liver / Q000201:enzymology*; D008297:Male; D051381:Rats; D039181:Steroid 16-alpha-Hydroxylase; D013250:Steroid Hydroxylases / Q000378:metabolism*; D013737:Testis / Q000502:physiology*
D003687:Dehydroepiandrosterone; D013250:Steroid Hydroxylases; D001189:Aryl Hydrocarbon Hydroxylases; C450279:CYP2C11 protein, rat; D000072467:Cytochrome P450 Family 2; D039181:Steroid 16-alpha-Hydroxylase; D000633:Aminopyrine N-Demethylase