Estimation of protein-ligand binding parameters from contiuous ultrafiltration results.
A method of analyzing the result of continuous ultrafiltration experiments to obtain protein-ligand binding parameters is presented. This method employs a nonlinear least-squares regression algorithm coupled with a model of protein-ligand binding which alloww the computation of free ligand concentrations, and a second-order Runge-Kutta method to integrate free concentrations with respect to collected ultrafiltrate. The approach is general and effectively removes the constraints on maximum fraction size imposed by other methods.
D000251:Adenosine Triphosphatases / Q000378:metabolism; D001665:Binding Sites; D003201:Computers; D008024:Ligands / Q000378:metabolism*; D008954:Models, Biological; D009183:Mycotoxins / Q000378:metabolism; D010456:Peptides, Cyclic / Q000378:metabolism; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D014462:Ultrafiltration
D008024:Ligands; D009183:Mycotoxins; D010456:Peptides, Cyclic; D011506:Proteins; D000251:Adenosine Triphosphatases