Detection of genetically determined histocompatibility antigen differences between HL-A identical and MLC nonreactive siblings.
Five patients with aplastic anemia were shown to be sensitized in vivo to the transplantation antigens of HL-A identical and MLC nonreactive sibling bone marrow transplantation donors. The sensitization in four cases were detected by antibody-dependent cell-mediated lysis (AICML) techniques, and in one case by antibody-dependent cell-mediated lysis (ADCML). In two cases the inherited nature of the sensitizing antigen was demonstrated, and in one case the inheritance of the antigen was shown by a family study to be independent of the HL-A and MLC loci. Maximal AICML was demonstrated in two cases at the time of bone marrow graft rejection. Successful transplants after immunosuppression with rabbit anti-thymocyte serum, procarbazine, and cyclophosphamide were performed in two patients, in whom sensitization was detected before transplantation.
D000918:Antibody Specificity; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D003601:Cytotoxicity Tests, Immunologic; D005260:Female; D005838:Genotype; D006084:Graft Rejection; D006680:HLA Antigens; D006649:Histocompatibility Antigens; D006801:Humans; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D014184:Transplantation, Homologous
D006680:HLA Antigens; D006649:Histocompatibility Antigens