title: Characterization of a bacteriophage T4 mutant lacking DNA-dependent ATPase.
abstract: A DNA-dependent ATPase has previously been purified from bacteriophage T4-infected Escherichia coli. A mutant phage strain lacking this enzyme has been isolated and characterized. Although the mutant strain produced no detectable DNA-dependent ATPase, growth properties were not affected. Burst sizes were similar for the mutant phage and T4D in polA1, recB, recC, uvrA, uvrB, uvrC, and various DNA-negative E. coli. UV sensitivity and genetic recombination were normal in a variety of E. coli hosts. Mapping data indicate that the genetic locus controlling the mutant occurs near gene 56. The nonessential nature of this gene is discussed.
mesh_terms: D000251:Adenosine Triphosphatases / Q000096:biosynthesis* / Q000378:metabolism; D000818:Animals; D002417:Cattle; D002474:Cell-Free System; D002848:Chromatography, DEAE-Cellulose; D002874:Chromosome Mapping; D003090:Coliphages / Q000201:enzymology* / Q000254:growth & development / Q000528:radiation effects; D004247:DNA / Q000378:metabolism; D004267:DNA Viruses; D005796:Genes; D008040:Genetic Linkage; D006898:Hydroxylamines; D009153:Mutagens; D009154:Mutation; D011830:Radiation Effects; D011995:Recombination, Genetic; D013950:Thymus Gland; D014466:Ultraviolet Rays; D014779:Virus Replication
chemical_list: D006898:Hydroxylamines; D009153:Mutagens; D004247:DNA; D000251:Adenosine Triphosphatases