Functional activities of rosette separated human peripheral blood leukocytes.
Subpopulations of human peripheral blood leukocytes were isolated by rosette formation and tested for functional activity. E -rosette-forming cells (E-RFC) and EAC-RFC were separated from non-resetting cells by sedimentation on Ficoll-Hypaque gradients. The efficiency of the method and the purity of the resulting subpopulations were high. E-RFC responded to PHA Con A, allogeneic leukocytes, and PPD, with higher levels of proliferative reactivity than the unseparated lymphocytes while E-RFC depleted, EAC-RFC, and null cells showed only low levels of reactivity. Reactivity to PWM and tetanus toxoid was also restricted to the E-RFC subpopulation, but was lower than that of unseparated cells. A staphylococcal antigen preparation triggered lymphoproliferative reactivity in the E-RFC, E-RFC depleted, EAC-RFC, and the null cell subpopulations. 51Cr release lymphocyte cytotoxicity against a human lymphoblast target cell line was found in the E-RFC and null cell fractions but was not observed with the EAC-RFC subpopulation.
D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D003208:Concanavalin A / Q000494:pharmacology; D003601:Cytotoxicity Tests, Immunologic; D004912:Erythrocytes / Q000276:immunology; D004950:Esterases / Q000032:analysis; D005455:Fluorescent Antibody Technique; D006801:Humans; D007104:Immune Adherence Reaction; D037102:Lectins / Q000494:pharmacology; D007962:Leukocytes / Q000276:immunology*; D008213:Lymphocyte Activation / Q000187:drug effects; D007959:Lymphocyte Culture Test, Mixed; D008264:Macrophages / Q000276:immunology; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D013204:Staphylococcus Phages / Q000276:immunology; D013745:Tetanus Toxoid; D014373:Tuberculin
D037102:Lectins; D011947:Receptors, Antigen, B-Cell; D013745:Tetanus Toxoid; D014373:Tuberculin; D003208:Concanavalin A; D004950:Esterases