Potent inhibition of dynein adenosinetriphosphatase and of the motility of cilia and sperm flagella by vanadate.
The motility of demembranated sea urchin sperm flagella and that of embryo cilia reactivated with 0.1 mM ATP are completely inhibited by 4 micron and 0.5 micron vanadium(V) [V(V), in vanadate], respectively. The Mg2+-activated ATPase activity (ATP phosphohydrolase, EC 3.6.1.3)of the latent form of dynein 1 is inhibited 50% by 0.5-1 micron V(V), while the Ca2+-activated ATPase activity is much less sensitive. The inhibition of flagellar beat frequency and of dynein 1 ATPase activity by V(V) appears not to be competitive with ATP. In agreement with other reports, the inhibition of (Na,K)-ATPase by V(V) shows a slow onset in the presence of ATP and is relatively rapid in its absence. With dynein, however, the inhibition occurs at a rapid rate whether or not ATP is present. Catechol at a concentration of 1 mM reverses the V(V) inhibition of reactivated sperm motility, dynein ATPase, and (Na, K)-ATPase. Myosin and actomyosin ATPases show no inhibition by concentrations of V(V) up to 500 micron. The inhibition by V(V) provides a possible technique for distinguishing between the actions of dynein and myosin in different forms of cell motility.
D000205:Actomyosin / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors*; D000818:Animals; D002396:Catechols / Q000494:pharmacology; D002923:Cilia / Q000187:drug effects; D004398:Dyneins / Q000037:antagonists & inhibitors*; D005407:Flagella / Q000187:drug effects; D008297:Male; D009218:Myosins / Q000378:metabolism; D011188:Potassium / Q000378:metabolism; D012617:Sea Urchins; D012964:Sodium / Q000378:metabolism; D013081:Sperm Motility / Q000187:drug effects; D014639:Vanadium / Q000494:pharmacology*
D002396:Catechols; D014639:Vanadium; D000205:Actomyosin; D012964:Sodium; D000251:Adenosine Triphosphatases; D009218:Myosins; D004398:Dyneins; D011188:Potassium