[Effectivity of murein preparations in the phage inhibition test--effect of Ca++ions and EDTA].
The inactivating efficacy for the typing phages 54, 83A, and 187 of the peptidoglycan of Staphylococcus aureus is decreased by increasing the Ca++-content of the medium; the irreversible inhibition of phage 187 becomes reversible. The percentage of its inhibition is proportional to the Ca++-content of the medium. Minute amounts of phage 187 bound irreversibly under Ca++-deprivation can be loosened by addition of Ca++-ions. Addition of EDTA up to the equivalence concentration of Ca++ present in the medium has no influence. Addition of EDTA up to the tenfold equivalence causes a significant increase of the inactivating efficacy of the peptidoglycan.
D001434:Bacteriophage Typing; D002118:Calcium / Q000494:pharmacology*; D002413:Cations, Divalent; D004492:Edetic Acid / Q000494:pharmacology*; D010457:Peptidoglycan / Q000494:pharmacology*; D013204:Staphylococcus Phages / Q000187:drug effects*; D013211:Staphylococcus aureus
D002413:Cations, Divalent; D010457:Peptidoglycan; D004492:Edetic Acid; D002118:Calcium
Wirksamkeit von Mureinpräparationen im Phagenhemmtest--Einfluss von Ca++-Ionen und EDTA.