The isolation and characterization of the plasma membrane of cultured chinese hamster ovary cells.
Plasma membranes were prepared from cultured Chinese hamster ovary cells utilizing a two-phase polymer system and were characterized by enzymatic and chemical assay, and by electron microscopy. The usual degree of purification of presumptive membrane markers such as Na+-K+ ATPase (ATP phosphohydrolase, EC 3.6.1.3) ranged from three-to eightfold. Gel electrophoresis in SDS revealed several polypeptides and two glycopeptides which were enriched in the plasma membrane fraction.
D000251:Adenosine Triphosphatases / Q000032:analysis; D002458:Cell Fractionation / Q000379:methods; D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology / Q000648:ultrastructure*; D008970:Molecular Weight; D009251:NADPH-Ferrihemoprotein Reductase / Q000032:analysis; D009708:Nucleotidases / Q000032:analysis; D013385:Succinate Dehydrogenase / Q000032:analysis
D013385:Succinate Dehydrogenase; D009251:NADPH-Ferrihemoprotein Reductase; D009708:Nucleotidases; D000251:Adenosine Triphosphatases