Kinetic investigation of the interaction of cibacron blue F3G-A with phosphofructokinase from yeast.
Yeast phosphofructokinase is strongly inhibited by Cibacron Blue F3G-A. The inhibition is competitive in respect to the phosphate donor. Fructose 6-phosphate and ATP are able to abolish the dye-inhibition. Replacement of the strong inhibitor ATP by ITP as phosphate donor gives qualitatively analogous effects. The influence of Cibacron Blue F3G-A on the kinetic pattern of yeast phosphofructokinase can be described in terms of the kinetic model of Freyer et al. [8] if one assumes that the dye binds to the ATP-binding sites in a competitive manner.
D000873:Anthracenes / Q000494:pharmacology*; D004396:Coloring Agents / Q000494:pharmacology*; D007700:Kinetics; D008433:Mathematics; D010732:Phosphofructokinase-1 / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D014227:Triazines / Q000494:pharmacology
D000873:Anthracenes; D004396:Coloring Agents; D014227:Triazines; C012842:Cibacron Blue F 3GA; D010732:Phosphofructokinase-1