Decreased glycolytic flux rate in the isolated perfused rat brain after pretreatment with 6-aminonicotinamide.
Cerebral energy metabolism was studied in the isolated perfused rat brain after 6-aminonicotinamide (6-AN; 35 mg/kg i.p.) administered to the intact animals 7 hrs before perfusion was started. The metabolic alterations in the isolated rat brains were such as reported for rat and mouse brain in vivo: Inhibition of 6-phosphogluconate dehydrogenase was followed by an accumulation of 6-phosphogluconate, leading to a decreased activity of glucosephosphate isomerse. This was reflected by increased levels of glucose and glucose 6-phosphate and decreased levels of fructose 6-phosphate, pyruvate and lactate. Since the concentration of lactate in the perfusate of the isolated brain was also lowered, 6-AN must have reduced the glycolytic flux rate.
D015120:6-Aminonicotinamide / Q000494:pharmacology*; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D004734:Energy Metabolism / Q000187:drug effects; D005956:Glucose-6-Phosphate Isomerase / Q000378:metabolism; D006019:Glycolysis / Q000187:drug effects*; D066298:In Vitro Techniques; D007773:Lactates / Q000378:metabolism; D009536:Niacinamide / Q000031:analogs & derivatives*; D010477:Perfusion; D010734:Phosphogluconate Dehydrogenase / Q000378:metabolism; D011773:Pyruvates / Q000378:metabolism; D051381:Rats
D007773:Lactates; D011773:Pyruvates; D009536:Niacinamide; D015120:6-Aminonicotinamide; D010734:Phosphogluconate Dehydrogenase; D005956:Glucose-6-Phosphate Isomerase