title: A comparison of bovine nasal cartilage proteoglycan core protein produced by chondroitinase and hyaluronidase: the possible role of protease contaminants.
abstract: The basic subunit of cartilage proteoglycan consists of multiple glycosaminoglycan chains covalently attached to a core protein. It is unclear as to whether there is a single core protein or multiple different core proteins, since previous studies using either chondroitinase or testicular hyaluronidase to enzymatically remove chondroitin sulfate side chains from the proteoglycan subunit have yielded conflicting results. In the present study, a chondroitinase-produced core protein preparation isolated as a single peak on Sepharose gel chromatography was found to contain at least two immunologically distinct components. Hyaluronidase-produced core protein from the same proteoglycan subunit fraction was found to contain multiple components nearly all of which were smaller than the components in the chondroitinase digest. A possible explanation of these findings is that they resulted from proteolytic degradation of the core protein in the course of the enzymatic removal of its chondroitin sulfate. The presence of small amounts of protease contaminants in several commercial chondroitinase and hyaluronidase preparations was detected by an extremely sensitive radioassay. Until proteases can be rigorously excluded from enzyme preparations used to degrade the proteoglycan subunit, it will not be possible to determine whether it consists of a single or several different core proteins.
mesh_terms: D000818:Animals; D002356:Cartilage; D002417:Cattle; D002810:Chondroitinases and Chondroitin Lyases; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006821:Hyaluronoglucosaminidase; D005779:Immunodiffusion; D009666:Nose; D010447:Peptide Hydrolases / Q000032:analysis*; D011509:Proteoglycans / Q000032:analysis*
chemical_list: D011509:Proteoglycans; D006821:Hyaluronoglucosaminidase; D010447:Peptide Hydrolases; D002810:Chondroitinases and Chondroitin Lyases