Effects of 3'-methyl-4-dimethylaminoazobenzene and 4-aminoazobenzene on plating efficiency, mitotic index, and macromolecular synthesis of cultured liver cells.
The response of a cultured liver cell line to azo dyes was investigated by the application of 3'-methyl-4-dimethylaminoazobenzene (3'-Me-DAB) and 4-aminoazobenzene (AB) with observation of, in particular, the changes which occurred in plating efficiency, macromolecular synthesis and mitotic indices. The results were as follows. (1) The application of 3'-Me-DAB resulted in a decrease in size of colonies and plating efficiency of both J-5-2 cells (derived from 7-day-old rat liver tissues) and cells of the colonical clone, J-5-2cl. (2) AB caused greater inhibition of plating efficiency than did 3'-Me-DAB. (3) Both 3'-Me-DAB and AB inhibited synthesis of DNA but not of RNA or of protein. (4) AB exhibited a greater inhibitory effect on mitotic indices than did 3'-Me-DAB.
D000818:Animals; D001391:Azo Compounds / Q000494:pharmacology*; D002460:Cell Line; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D066298:In Vitro Techniques; D008099:Liver / Q000166:cytology*; D008938:Mitosis / Q000187:drug effects*; D008940:Mitotic Index / Q000187:drug effects*; D051381:Rats; D010128:p-Aminoazobenzene / Q000494:pharmacology*; D004124:p-Dimethylaminoazobenzene / Q000494:pharmacology*
D001391:Azo Compounds; D010128:p-Aminoazobenzene; D004247:DNA; D004124:p-Dimethylaminoazobenzene