[Production of an anti human B lymphocyte serum for detection of B cell-specific membrane antigens (author's transl)].
Rabbits were immunized with lymphocytes from patient with chronic lymphatic leukemia (CLL), who showed a remarkable lymphocytosis of B cell origin (distinct membrane fluorescence). After absorption with AB-erythrocytes, kidney tissue powder and infant thymocytes specificity of the antiserum was demonstrated by examination of normal lymphocytes, thymocytes and different CLL-lymphocytes (B- and T-cell leukemias) using indirect membrane fluorescence.
D000818:Animals; D000918:Antibody Specificity; D000961:Antilymphocyte Serum; D001402:B-Lymphocytes / Q000276:immunology*; D006801:Humans; D007945:Leukemia, Lymphoid / Q000097:blood; D011817:Rabbits / Q000276:immunology
D000961:Antilymphocyte Serum
Herstellung eines Antiserums gegen menschliche B-Lymphozyten zum Nachweis B-zellspezifischer Membranantigene.