Asimple test for immunogenicity of colloidal infusion solutions- the draining lymph node activation.
The increasing number of lymphocytes with nucleoli synthesizing RNA in the mouse lymph nodes, draining the site of injection of infusion solutions, was used as a marker for their immunogenicity. The percentage of "active" lymphocytes significantly increased 3 days after the administration of preparations based on bovine serum or human haemoglobin which were found immunogenic when testing for antibody formation. Such a reaction was not elicited in mice treated with Physiogel, Dextran and Duxon which do not cause any production of antibodies. The described test may serve as a rapid and economical assay for the immunogenicity of infusion solutions and other substances.
D000818:Animals; D002466:Cell Nucleolus; D003102:Colloids; D006454:Hemoglobins / Q000378:metabolism; D007263:Infusions, Parenteral; D008198:Lymph Nodes / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008808:Mice, Inbred CBA; D012313:RNA / Q000096:biosynthesis; D012710:Serum Albumin, Bovine / Q000008:administration & dosage; D012996:Solutions
D003102:Colloids; D006454:Hemoglobins; D012996:Solutions; D012710:Serum Albumin, Bovine; D012313:RNA