title: Fructose metabolism in four Pseudomonas species.
abstract: 1. ATP-Dependent phosphorylation of fructose could not be detected in extracts of fructose-grown cells of Pseudomonas extorquens strain 16, Pseudomonas 3A2, Pseudomonas acidovorans and Pseudomonas fluorescens. Instead, phosphorylation of fructose to fructose-1-phosphate was found to occur when cell-free extracts were incubated with fructose and phosphoenolpyruvate. Such an activity could not be detected in cell-free extracts of succinate-grown cells. 2. High levels of 1-phosphofructokinase were found in extracts of the above organisms when growth on fructose. 3. Mutants of Pseudomonas extorquens strain 16 lacking 1-phosphofructokinase were unable to grow on fructose. Revertants to growth on fructose had regained the capacity to synthesize this enzyme, indicating its necessary involvement in fructose metabolism. 4. A survey has been carried out of enzymes involved in carbohydrate metabolism in the species listed above.
mesh_terms: D002474:Cell-Free System; D003080:Cold Temperature; D005632:Fructose / Q000378:metabolism*; D005636:Fructosephosphates / Q000378:metabolism; D010728:Phosphoenolpyruvate / Q000096:biosynthesis; D010732:Phosphofructokinase-1 / Q000378:metabolism; D010770:Phosphotransferases / Q000378:metabolism*; D011549:Pseudomonas / Q000378:metabolism*; D011551:Pseudomonas fluorescens / Q000378:metabolism*; D013045:Species Specificity
chemical_list: D005636:Fructosephosphates; D005632:Fructose; D010728:Phosphoenolpyruvate; D010770:Phosphotransferases; D010732:Phosphofructokinase-1