Fluorimetric method for the detection of anti-DNA antibodies in serum.
A method for the detection of native anti-DNA antibodies in serum is described. The method is based on the reactivity of fluorescein isothiocyanate with DNA, forming a complex capable of combining with anti-DNA antibodies. The fluorescein content of the precipitated fluorescein-DNA anti-DNA complex is then measured in a fluorometer. The assay is accurate, highly reproducible, and inexpensive to perform. Comparative studies performed with the Farr assay show the fluorimetric method to be more sensitive in detecting anti-DNA antibodies in the serum of SLE patients.
D000974:Antibodies, Antinuclear / Q000032:analysis*; D000918:Antibody Specificity; D000936:Antigen-Antibody Complex; D004247:DNA / Q000276:immunology*; D005452:Fluoresceins; D005470:Fluorometry / Q000379:methods*; D005921:Glomerulonephritis / Q000276:immunology; D006801:Humans; D007105:Immune Complex Diseases / Q000276:immunology; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D011863:Radioimmunoassay
D000974:Antibodies, Antinuclear; D000936:Antigen-Antibody Complex; D005452:Fluoresceins; D004247:DNA