title: A simple freezing and storage method to preserve the stimulating function of leukocytes in the mixed leukocyte reaction.
abstract: Human peripheral blood leukocytes retain their capacity to function as stimulating cells in the one way MLR after freezing and storage at - 70 degrees C by a simple and inexpensive method. This storage method facilitates the use of a standard stimulating pool of cells from three donors which may provide a quantitative assay for testing the ability of cells to respond to allogeneic cells in culture.
mesh_terms: D005615:Freezing; D006801:Humans; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D013997:Time Factors; D014021:Tissue Preservation / Q000379:methods*