A histochemical study of the liver lesion induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (dioxin) in rats.
A histochemical study of plasma-membrane associated enzymes in rat liver demonstrated a significant lesion 3 days after a single oral dose of 2,3,7,8-tetrachlorodibenzo-p-dioxin (dioxin). The complete loss of canalicular ATPase reaction in the parenchymal cells of the centrilobular zone remained the prominent feature of the liver throughout the 6-wk period studied. Involvement of the periportal and midzonal regions occurred in moribund animals and improvement in the health of two surviving animals at 9 mth was associated with a normal distribution of ATPase in the liver. Qualitative changes in 5-nucleotidase and acid phosphatase were secondary to the parenchymal cell damage. This lesion supports the morphological evidence, reported previously, that the parenchymal cell plasma-membrane is a specific subcellular site of the toxic action of dioxin.
D000135:Acid Phosphatase / Q000032:analysis; D000251:Adenosine Triphosphatases / Q000032:analysis; D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D056486:Chemical and Drug Induced Liver Injury; D004147:Dioxins / Q000633:toxicity*; D005260:Female; D006651:Histocytochemistry; D008099:Liver / Q000201:enzymology; D008107:Liver Diseases / Q000201:enzymology; D009708:Nucleotidases / Q000032:analysis; D000072317:Polychlorinated Dibenzodioxins / Q000633:toxicity*; D051381:Rats; D013997:Time Factors
D004147:Dioxins; D000072317:Polychlorinated Dibenzodioxins; D009708:Nucleotidases; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D000251:Adenosine Triphosphatases