Calcium-accumulating properties of subcellular fractions of bovine vascular smooth muscle.
This study examined the calcium transport properties of two subcellular fractions of bovine aorta obtained by differential centrifugation. The vesicular fraction had a high-affinity (Km = 1.05 X 10(-6)M) calcium transport mechanism which could be potentiated by using the calcium-precipitating anion, oxalate. The mitochondria-enriched fraction's calcium transport system had a lower affinity for calcium than did that of the vesicular fraction. The calcium capacity of the vesicular fraction was determined by comparing the steady-state calcium uptake in the presence of oxalate in the whole homogenate with the same uptake by the vesicular fraction alone. A calcium capacity of about 100 mumoles Ca2+/kg aorta was obtained. It is concluded that the vesicular fraction has  all of the properties of a major site of subcellular calcium regulation in vascular smooth muscle.
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D001011:Aorta / Q000648:ultrastructure*; D002118:Calcium / Q000378:metabolism*; D002417:Cattle; D004533:Egtazic Acid / Q000494:pharmacology; D007700:Kinetics; D008931:Mitochondria, Muscle / Q000378:metabolism; D009130:Muscle, Smooth / Q000378:metabolism / Q000648:ultrastructure*; D013347:Subcellular Fractions / Q000378:metabolism
D004533:Egtazic Acid; D000251:Adenosine Triphosphatases; D002118:Calcium