Localization on mitochondrial DNA of mutations leading to a loss of rutamycin-sensitive adenosine triphosphatase.
Four cytoplasmic mutants of Saccharomyces cerevisiae showing loss of mitochondrial rutamycin-sensitive ATPase activity but having significant cytochrome oxidase and NADH-cytochrome c reductase have been isolated. Genetic studies indicate the mutations to be closely linked to each other and have been assigned to a new locus, PHO1. The mutations show a low frequency of recombination with the OL12 locus, suggesting a linkage to this marker. They are not, however, linked to the OLI1 locus. Linkage of the ATPase mutations to the OLI2 locus is also indicated by restoration of wild-type diploids by sigma- clones that retain the segment of mitochondrial DNA carrying OLI2. Based on the recombinants issued from crosses of the mutants with a triple drug-resistant strain and an analysis of the resistance markers present in sigma- clones that are effective in restoring a wild-type phenotype, the PHO1 locus has been placed in the segment of DNA located between PAR1 and OLI2.
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000935:Antifungal Agents / Q000494:pharmacology*; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D003579:Cytochrome Reductases / Q000378:metabolism; D004272:DNA, Mitochondrial / Q000378:metabolism*; D005838:Genotype; D005990:Glycerol / Q000378:metabolism; D008928:Mitochondria / Q000201:enzymology; D009154:Mutation; D011995:Recombination, Genetic; D012427:Rutamycin / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013045:Species Specificity; D013053:Spectrophotometry
D000935:Antifungal Agents; D004272:DNA, Mitochondrial; D012427:Rutamycin; D003579:Cytochrome Reductases; D000251:Adenosine Triphosphatases; D005990:Glycerol