Solvolytic desulfation of glycosaminoglycuronan sulfates with dimethyl sulfoxide containing water or methanol.
A solvolytic desulfation of glycosaminoglycuronan sulfates was developed by treatment of their pyridinium salts with dimethyl sulfoxide containing 10% of water or methanol at 80-100 degrees. Chemical and physical studies showed that the solvolytic desulfation is a useful method applicable to all the known glycosaminoglycuronan sulfates without producing depolymerization or unfavorable chemical changes in the polysaccharide molecules. An almost completely desulfated, N-acetylated heparin (S: 0.12%) was obtained by treatment of an N-desulfated and N-acetylated heparin with dimethyl sulfoxide containing 10% of methanol for 2 h at 100 degrees.
D004121:Dimethyl Sulfoxide; D006025:Glycosaminoglycans; D006493:Heparin; D006358:Hot Temperature; D006868:Hydrolysis; D000432:Methanol; D013431:Sulfates; D014867:Water
D006025:Glycosaminoglycans; D013431:Sulfates; D014867:Water; D006493:Heparin; D000432:Methanol; D004121:Dimethyl Sulfoxide