Mg2+- and Ca2+-stimulated ATPase at the outer surface of human peripheral lymphocytes and hematopoietic cell lines. Correlation between enzyme activity and immunoglobulin secretion.
A Mg2+- and Ca2+-dependent ATPase at the outer surface of human peripheral lymphocytes and hematopoietic cell lines has been studied. The enzyme activity varied about 60 fold between the extremes of the cell lines tested. There was no simple relationship between surface-ATPase activity and cell surface area. Nor was the presence of villi or any other morphological characteristic at the cell surface decisive for the degree of surface-ATPase activity. A correlation was, however, found between the ATPase activity and a phenomenon probably involving a contractile process namely immunoglobulin secretion, but it was not possible to establish an interdependence of these cell characteristics.
D000251:Adenosine Triphosphatases / Q000097:blood*; D002118:Calcium / Q000494:pharmacology*; D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology; D006412:Hematopoietic Stem Cells / Q000201:enzymology* / Q000276:immunology; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis*; D008214:Lymphocytes / Q000201:enzymology* / Q000276:immunology; D008274:Magnesium / Q000494:pharmacology*
D007136:Immunoglobulins; D000251:Adenosine Triphosphatases; D008274:Magnesium; D002118:Calcium