[Characterization of messenger ribonucleoproteins from yeasts (author's transl)].
A method to produce in 15 min stable sphaeroplasts from yeast of the early log-phase is described. This method was used to isolate polysomes, after specific radioactive labelling of mRNA. The mRNP *-particles, isolated from polysomes, have sedimentation-coefficients from 10 to 80S and a density of 1.4 g/cm3, the polysomes a density of 1.58 g/cm3 and the ribosomes a density of 1.6. g/cm3.
D009698:Nucleoproteins; D011132:Polyribosomes; D012333:RNA, Messenger; D012261:Ribonucleoproteins; D012441:Saccharomyces cerevisiae; D013104:Spheroplasts
D009698:Nucleoproteins; D012333:RNA, Messenger; D012261:Ribonucleoproteins
Charakterisierung von Messenger-Ribonukleoproteiden aus Hefezellen