Rat liver homogenate hydrolases splitting N-acetyl-L-tyrosine ethyl ester.
Using gel filtration on Sephadex G 200, three fractions splitting N-acetyl-L-tyrosine ethyl ester (ATEE), termed I, S and II, were found in rat liver homogenate. In molecular size, electrophoretic mobility and thermolability, fractions I and II correspond to the ATEE-splitting enzymes contained in rat serum. The liver fraction S had a different surface electric charge and was relatively stable at high temperatures. Apart from ATEE, it splits certain ester and amide bonds of synthetic substrates. Purified fraction S increased vascular permeability in guinea pigs. Differential centrifugation of liver homogenate showed that fraction S and fractions I and II were localized differently in the subcellular particles.
D000085:Acetates / Q000378:metabolism; D000818:Animals; D002850:Chromatography, Gel; D004952:Esters / Q000378:metabolism; D006867:Hydrolases / Q000378:metabolism*; D008099:Liver / Q000201:enzymology* / Q000378:metabolism; D051381:Rats; D014443:Tyrosine / Q000378:metabolism*
D000085:Acetates; D004952:Esters; D014443:Tyrosine; D006867:Hydrolases