The influence of oxygen on nitrite reduction in a reconstituted system.
Data regarding the role of oxygen in nitrite reduction are presented. In an NADPH-generating system including homogeneously purified ferredoxin-NADP reductase, ferredoxin (or flavodoxin) and nitrite reductase from the alga Bumilleriopsis filiformis, oxygen and nitrite can be reduced simultaneously. In air, rates of 1.2 mumol nitrite reduced-min-1-mg-1 nitrite reductase are obtained, which are physiologically feasible. Ferredoxin is inhibited non-competitively by oxygen during nitrite reduction. Oxygen uptake due to the oxidase reaction of ferredoxin-NADP reductase mediated by flavodoxin from Chlorella fusca and ferredoxin from Bumilleriopsis involves superoxide and is inhibited by the nitrite reducing system.
D000693:Anaerobiosis; D056890:Eukaryota / Q000378:metabolism*; D005287:Ferredoxin-NADP Reductase / Q000378:metabolism; D005288:Ferredoxins / Q000494:pharmacology; D005418:Flavodoxin / Q000494:pharmacology; D007700:Kinetics; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D009572:Nitrite Reductases / Q000378:metabolism*; D010100:Oxygen / Q000494:pharmacology*; D010101:Oxygen Consumption; D013482:Superoxide Dismutase / Q000494:pharmacology
D005288:Ferredoxins; D005418:Flavodoxin; D013482:Superoxide Dismutase; D005287:Ferredoxin-NADP Reductase; D009247:NADH, NADPH Oxidoreductases; D009572:Nitrite Reductases; D010100:Oxygen