title: RNA synthesis in mitochondria isolated from rat liver.
abstract: Mitochondrial RNA (mtRNA) was synthesized from purine and pyrimidine nucleosides in coupling with oxidative phosphorylation using isolated mitochondria. The in vivo synthesized mtRNA was adenine-uracil rich and sedimented at about 20 S by sucrose density gradient centrifugation. A major part of the newly synthesized mtRNA was shown to be poly (A)-containing RNA by the resistance to the digestion with pancreatic RNase and RNase T1 and the affinity to poly (U)-Sepharose columns or Millipore filters.
mesh_terms: D000818:Animals; D066298:In Vitro Techniques; D008297:Male; D008930:Mitochondria, Liver / Q000378:metabolism*; D010085:Oxidative Phosphorylation; D011684:Purine Nucleosides / Q000378:metabolism; D011741:Pyrimidine Nucleosides / Q000378:metabolism; D012313:RNA / Q000096:biosynthesis*; D051381:Rats
chemical_list: D011684:Purine Nucleosides; D011741:Pyrimidine Nucleosides; D012313:RNA