title: Glycolytic and tricarboxylic acid cycle enzyme activities during intraperiplasmic growth of Bdellovibrio bacteriovorus on Escherichia coli.
abstract: Selected enzyme activities were measured in extracts of the total cell pellets obtained at various times during aerobic intraperiplasmic growth of Bdellovibrio bacteriovorus 109J on anaerobically grown Escherichia coli substrate cells. Initially, the glycolytic enzyme activities were associated with the input of E. coli and the tricarboxylic acid cycle enzyme activities with the input of bdellovibrios. During the first 90 min of Bdellovibrio development, the glycolytic activities declined about 25 to 60%, whereas the tricarboxylic acid cycle activities increased about 10%. Between 110 and 180 min, the glycolytic activities decreased to trace levels and tricarboxylic acid cycle activities increased about 50 to 90%. Both bdellovibrio cell extracts and the cell-free growth menstruum (obtained after bdellovibrio growth on E. coli) caused the inactivation of glycolytic enzymes in E. coli extracts.
mesh_terms: D001501:Bdellovibrio / Q000201:enzymology* / Q000254:growth & development; D002952:Citric Acid Cycle; D004926:Escherichia coli / Q000201:enzymology; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism; D005696:Galactosidases / Q000378:metabolism; D006019:Glycolysis; D007700:Kinetics; D010101:Oxygen Consumption; D010447:Peptide Hydrolases / Q000378:metabolism; D010732:Phosphofructokinase-1 / Q000378:metabolism
chemical_list: D010732:Phosphofructokinase-1; D005696:Galactosidases; D010447:Peptide Hydrolases; D005634:Fructose-Bisphosphate Aldolase