title: Studies on assays for plasma prekallikrein and for the monitoring of coumarol therapy.
abstract: Chromogenic substrates were used to assay prekallikrein, prothrombin and factor X. Plasma prekallikrein was contact activated and allowed the splitting of HD-Pro-Phe-Arg-PNA which has affinity for plasma kallikrein. Prothrombin was assayed in various ways (immunologically, after activation with Ecarin or in a less specific way). Determination of factor X is considered as a possible specific method in the monitoring of coumarol therapy.
mesh_terms: D003374:Coumarins / Q000627:therapeutic use*; D005170:Factor X / Q000037:antagonists & inhibitors; D005175:Factor XII Deficiency / Q000201:enzymology; D005341:Fibrinolysin / Q000494:pharmacology; D006801:Humans; D007610:Kallikreins / Q000032:analysis*; D011288:Prekallikrein / Q000032:analysis*; D011516:Prothrombin / Q000037:antagonists & inhibitors; D012910:Snake Venoms; D013917:Thrombin / Q000494:pharmacology
chemical_list: D003374:Coumarins; D012910:Snake Venoms; D011516:Prothrombin; D005170:Factor X; D011288:Prekallikrein; D007610:Kallikreins; D013917:Thrombin; D005341:Fibrinolysin