Calcium-dependent adenosine triphosphatase activity preservation in isolated sarcoplasmic reticulum.
ATPase activity in sarcoplasmic reticulum vesicles was measured before and after storage for several weeks and under a variety of conditions. Rapid freezing and storage at-80 degrees C provided optimum protection of enzyme activity. Sarcoplasmic reticulum preparations stored at 0 degrees C or frozen slowly and stored at-20 degrees C were not stable. At 0 degrees C sucrose, glycerol, and dithiothreitol had a stabilizing effect while NaCl, dimethylsulfoxide, and antioxidants afforded little or no protection.
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004229:Dithiothreitol / Q000494:pharmacology; D005615:Freezing; D005990:Glycerol / Q000494:pharmacology; D008121:Nephropidae; D012519:Sarcoplasmic Reticulum / Q000201:enzymology*; D012965:Sodium Chloride / Q000494:pharmacology; D013395:Sucrose / Q000494:pharmacology; D013696:Temperature; D014021:Tissue Preservation / Q000379:methods
D012965:Sodium Chloride; D013395:Sucrose; D000251:Adenosine Triphosphatases; D005990:Glycerol; D002118:Calcium; D004229:Dithiothreitol; D004121:Dimethyl Sulfoxide