title: Characterization of ATPases of plain synaptic vesicle and coated vesicle fractions isolated from rat brains.
abstract: The plain synaptic vesicle and the ocated vesicle fractions were isolated from rat brains, and the ATPase [EC 3.6.1.3] activities were characterized in terms of ionic effects, drug effects, and protein components. Coated vesicle fraction contained three times as much actomysin-like proteins as plain vesicle fraction, although both fractions had an identical ratio of actin-like protein to myosin-like protein. The ATPases of these two fractions were activated by both Mg2+ and Ca2+, and, in the presence of either of the cations, were inhibited by KCl. Reserpine activated plain vesicle ATPase only in the presence of Cl-. Colchicine and vinblastine inhibited coated vesicle ATPase only. The results are consistent with the view that actomyosin-like proteins are involved in the synaptic retrieval process.
mesh_terms: D000199:Actins / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D000838:Anions; D001921:Brain / Q000201:enzymology*; D002118:Calcium / Q000494:pharmacology; D003078:Colchicine / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D008970:Molecular Weight; D009218:Myosins / Q000378:metabolism; D009994:Osmolar Concentration; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats; D012110:Reserpine / Q000494:pharmacology; D013572:Synaptic Vesicles / Q000187:drug effects / Q000201:enzymology*; D014747:Vinblastine / Q000494:pharmacology
chemical_list: D000199:Actins; D000838:Anions; D014747:Vinblastine; D011189:Potassium Chloride; D012110:Reserpine; D000251:Adenosine Triphosphatases; D009218:Myosins; D008274:Magnesium; D003078:Colchicine; D002118:Calcium