Is there a plasma membrane-located anion-sensitive ATPase? III. Identity of the erythrocyte enzyme with (Ca2+ + Mg2+)-ATPase.
The characteristics of the anion-sensitive Mg2+-ATPase activity of the rabbit erythrocyte have been studied in a lyophilized ghost preparation. The enzyme appears to be different from the anion-sensitive Mg2+-ATPase activity of other tissues in many parameters, such as optimal pH, effects of various anions, oligomycin sensitivity and effects of Triton X-100. The enzyme is insensitive towards inhibition by irreversibly bound 4,4'-diisothiocyano-dihydrostilbene-2,2'-disulfonic acid (H2DIDS). This excludes a relationship between the enzyme and the "band 3" protein, which is thought to be involved in the anion exchange over the erythrocyte membrane. From the effects of ethyleneglycol-bis-(beta-aminoethylether)-N,N'-tetraacetic acid (EGTA), CaCl2, chlorpromazine and ruthenium red it is concluded that the enzyme activity does not represent a separate entity but is part of the (Ca2+ + Mg2+)-ATPase system of the erythrocyte membrane. A reported stimulatory effect of carbonic anhydrase is attributed to a contamination of the carbonic anhydrase preparation by calcium and/or (Ca2+ + Mg2+)-ATPase activator protein.
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D001557:Benzenesulfonates / Q000494:pharmacology; D001639:Bicarbonates / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002122:Calcium Chloride / Q000494:pharmacology; D002256:Carbonic Anhydrases / Q000494:pharmacology; D004910:Erythrocyte Membrane / Q000201:enzymology*; D004912:Erythrocytes / Q000201:enzymology*; D008274:Magnesium / Q000494:pharmacology; D009840:Oligomycins / Q000494:pharmacology; D010042:Ouabain / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000494:pharmacology; D013267:Stilbenes / Q000494:pharmacology; D013861:Thiocyanates / Q000494:pharmacology
D001557:Benzenesulfonates; D001639:Bicarbonates; D009840:Oligomycins; D013267:Stilbenes; D013861:Thiocyanates; D000001:Calcimycin; D011092:Polyethylene Glycols; D010042:Ouabain; D000251:Adenosine Triphosphatases; D002256:Carbonic Anhydrases; D008274:Magnesium; D002122:Calcium Chloride; D002118:Calcium