Acidic glycosaminoglycans in human kidney tissue.
The acidic glycosaminoglycans (AGAG) in normal human kidneys were fractionated on Dowex 1-X2 columns and analysed by electrophoretic separation in three buffers on cellulose acetate membranes and gel filtration on Sephadex G-100 columns, before and after digestion with chondroitinases and streptomyces hyaluronidase. Thin-layer chromatography was also performed to separate glucosamine from galactosamine moieties. Enzymatic digestion combined with electrophoretic characterization indicated that heparan sulfates exist as the main AGAG which accounted for two-fifths of the total AGAG. Hyaluronic acid and dermatan sulfates accounted for one-fourth and one-sixth of the total kidney AGAG, respectively. Chondroitin sulfate isomers (4-sulfate and 6-sulfate) consisted of the residual one-sixth of the total AGAG. An oversulfated chondroitin sulfate was detected in a small amount by demonstration of the unsaturated disulfated disaccharide after digestion with chondroitinase-ABC but not with chondroitinase-AC.
D000328:Adult; D004187:Disaccharides / Q000032:analysis; D005965:Glucuronates / Q000032:analysis; D006025:Glycosaminoglycans / Q000032:analysis* / Q000302:isolation & purification; D006595:Hexosamines / Q000032:analysis; D006801:Humans; D007668:Kidney / Q000032:analysis*; D008297:Male
D004187:Disaccharides; D005965:Glucuronates; D006025:Glycosaminoglycans; D006595:Hexosamines