title: Renal aminoacylase, a zinc enzyme.
abstract: Renal aminoacylase is inactivated by dialysis against metal complexing agents such as o-phenanthroline. Activity can be restored by addition of zinc ions. A zinc dissociation constant of about 10(-10)M at pH 7.8 is obtained by titration of the enzyme with a metal ion buffer. The reactivity of the SH groups of the enzyme is considerably affected by zinc ions. The enzyme contains two essential zinc ions per molecule.
mesh_terms: D000581:Amidohydrolases / Q000032:analysis / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D003545:Cysteine / Q000378:metabolism; D007668:Kidney / Q000201:enzymology*; D009646:Norleucine / Q000494:pharmacology; D013552:Swine; D015032:Zinc / Q000032:analysis / Q000378:metabolism
chemical_list: D009646:Norleucine; D000581:Amidohydrolases; D015032:Zinc; D003545:Cysteine