title: Mechanisms of siderophore iron transport in enteric bacteria.
abstract: Uptake of 55Fe- and 3H-labeled siderophores and their chronic analogues have been studied in Salmonella typhimurium LT-2 and Escherichia coli K-12. In S. typhimurium LT-2, at least two different mechanisms for siderophore iron transport may be operative. Uptake of 55Fe- and 3H-labeled ferrichrome and kinetically inert lambda-cis-chromic [3H]deferriferrichrome by the S. typhimurium LT-2 enb7 mutant, which is defective in the production of its native siderophore, enterobactin, appears to occur by two concurrent mechanisms. The first mechanism is postulated to involve either rapid uptake of iron released from the ferric complex by cellular reduction without penetration of the complex or ligand or dissociation of the complex and simultaneous uptake of both ligand and iron coupled with simultaneous expulsion of the ligand. The second mechanism appears to consist of slower uptake of the intact ferric complex.
mesh_terms: D001693:Biological Transport, Active; D004758:Enterobactin / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D005291:Ferrichrome / Q000031:analogs & derivatives / Q000378:metabolism*; D006877:Hydroxamic Acids / Q000378:metabolism*; D007501:Iron / Q000378:metabolism*; D007700:Kinetics; D009154:Mutation; D012486:Salmonella typhimurium / Q000378:metabolism*
chemical_list: D006877:Hydroxamic Acids; D005291:Ferrichrome; D004758:Enterobactin; D007501:Iron