title: Determination of the antigen binding capacity of antisera to collagen by gel filtration.
abstract: Significant differences in the antigen binding capacity of different antisera to collagen of identical haemagglutination titres were demonstrated by using gel filtration for the isolation of collagen-anticollagen immune-complexes. A partially degraded collagen was used as the antigen. The influence of antigen concentration on antigen binding capacity was investigated with 2 antisera of different avidity. The distribution of antibody-bound antigen among IgM- and IgG- peaks indicates the participation of both immunoglobulin classes in the formation of collagen-anticollagen immune-complexes.
mesh_terms: D000818:Animals; D000937:Antigen-Antibody Reactions; D001665:Binding Sites; D002850:Chromatography, Gel; D003094:Collagen; D003488:Cyanogen Bromide; D007106:Immune Sera; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011817:Rabbits / Q000276:immunology; D012995:Solubility
chemical_list: D007106:Immune Sera; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D003094:Collagen; D003488:Cyanogen Bromide