Standardization of an experimental immune uveitis in the rabbit for topical testing of drugs.
An egg albumin uveitis of the Arthus active type was developed in the rabbit. The experimental conditions were investigated in detail with regard to the following factors: influence of the number of sensitizing injections on serum antibody production, length of the recovery period which elapsed between sensitization and challenge, and influence of the size of the challenging dose on the severity of the inflammatory response. To develop the procedure optimally, emphasis was given to criteria of evaluation. Refractive index, protein and immunoglobulin assays in the aqueous humor of inflamed eyes were significantly correlated. These objective measures were considered more reliable than arbitrary grading systems. In addition, supportive histopathologic observations have been made in rabbit eyes. The above studies led to a reproducible model of uveitis in which drugs were tested topically. Dexamethasone phosphate in solution and indomethacin in suspension were effective in a dose-related manner. 6-Mercaptopurine did not demonstrate a useful anti-inflammatory effect.
D000287:Administration, Topical; D000818:Animals; D000906:Antibodies / Q000032:analysis; D001082:Aqueous Humor / Q000032:analysis / Q000276:immunology; D001183:Arthus Reaction / Q000188:drug therapy / Q000276:immunology* / Q000473:pathology; D003907:Dexamethasone / Q000627:therapeutic use; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D005136:Eye Proteins / Q000032:analysis; D005260:Female; D007136:Immunoglobulins / Q000032:analysis; D007213:Indomethacin / Q000627:therapeutic use; D008297:Male; D015122:Mercaptopurine / Q000627:therapeutic use; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology; D011817:Rabbits; D014605:Uveitis / Q000188:drug therapy / Q000276:immunology* / Q000473:pathology
D000906:Antibodies; D005136:Eye Proteins; D007136:Immunoglobulins; D003907:Dexamethasone; D010047:Ovalbumin; D015122:Mercaptopurine; D007213:Indomethacin