5-bromo-2'-deoxyuridine-stimulated calcium ion- or magnesium ion-dependent ecto-(adenosine triphosphatase) activity of cultured hamster cardiac cells.
1. Treatment of hamster heart cells in primary culture with 5-bromo-2'-deoxyuridine resulted in the greatly increased activity of a particulate Ca2+- or Mg2+-dependent ATPase (adenosine triphosphatase). 2. 5-Bromo-2'-deoxyuridine exerted these effects only when it was incorporated into cellular DNA, and then in a concentration-dependent  manner. 3. Serially replated cells contained less of the activity (expressed as a function of total cell protein) than did the primary cultures, but the stimulation caused by 5-bromo-2'-deoxyuridine addition was much greater. 4. The affected enzyme was apparently localized in the plasma membrane of the cells with its active centre exposed to the outer environment [ecto-(ATPase) dependent on Ca2+ or Mg2+].5. The activity was unaffected by treatment with p-chloromercuriphenylsulphonate, ouabain andverapamil. 6. Ecto (5'-nucleotidase) activity was not increased by 5-bromo-2'-deoxyuridine treatment of cells, and ecto-(p-nitrophenyl phosphatase) activity was only slightly enhanced.
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D001973:Bromodeoxyuridine / Q000494:pharmacology*; D002118:Calcium; D002478:Cells, Cultured; D006224:Cricetinae; D004247:DNA / Q000378:metabolism; D006321:Heart / Q000187:drug effects; D008274:Magnesium; D008647:Mesocricetus; D009206:Myocardium / Q000166:cytology / Q000201:enzymology*
D004247:DNA; D000251:Adenosine Triphosphatases; D001973:Bromodeoxyuridine; D008274:Magnesium; D002118:Calcium