Effect of spectinomycin on peptide chain initiation.
Spectinomycin inhibits the formation and causes dissociation of performed specific cell-free initiation complexes prepared with the trinucleotide A-U-G or R17 phage RNA, fmet-RNAF and either 30S ribosomal subunits or 70S ribosomes. Both the initiation factor and the Mg2+-induced processes are subject to inhibition by spectinomycin. The only exception is the Mg2+-induced 70S initiation system formed with A-U-G which is stimulated by spectinomycin.
D002474:Cell-Free System; D003062:Codon / Q000378:metabolism; D003090:Coliphages / Q000378:metabolism; D003864:Depression, Chemical; D004926:Escherichia coli / Q000378:metabolism; D008274:Magnesium / Q000494:pharmacology; D008715:Methionine / Q000378:metabolism; D010442:Peptide Chain Initiation, Translational / Q000187:drug effects*; D012343:RNA, Transfer / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism; D000198:Spectinomycin / Q000494:pharmacology*; D013997:Time Factors
D003062:Codon; D012343:RNA, Transfer; D000198:Spectinomycin; D008715:Methionine; D008274:Magnesium