[Extraction of highly active preparations of "soluble" Na+, K+-ATPase from different membrane stuctures of the brain].
A successive treatment with sodium iodide and digitonin of the myeline, microsome and synaptosome fractions of the cattle brain tissue resulted in obtaining the preparations of "soluble", Na+, K+-ATPase with a rather high specific activity non-containing practically Mg2+-dependent ATPase. To estimate the degree of these preparations structural heterogeneity they were subjected to analytical ultracentrifugation and disc-electrophoresis in polyacrylamide gel. The data obtained suggest an insignificant degree of heterogeneity for the "soluble" Na+, K+-ATP-ase preparations.
D000251:Adenosine Triphosphatases / Q000302:isolation & purification*; D000818:Animals; D001921:Brain / Q000201:enzymology* / Q000648:ultrastructure; D002417:Cattle; D004589:Electrophoresis, Disc; D008722:Methods; D008861:Microsomes / Q000201:enzymology; D009186:Myelin Sheath / Q000201:enzymology; D013574:Synaptosomes / Q000201:enzymology; D014461:Ultracentrifugation
D000251:Adenosine Triphosphatases
Oderzhannia vysokoaktyvnykh preparativ "rozchynnoï" Na+, K+-ATF-azy z riznykh membrannykh struktur mozku