[Spheroplasts of plague microbe strains from the Transcaucasian uplands and their capacity for pesticin synthesis].
Spherical formations of the plague microbe strains from the Transcaucasian Upland, I plague microbe strain of the sandwort variety and I strain of the marmot variety were obtained under the effect of lithium chloride. They had the remains of the cell wall, were viable, sensitive to osmotic shock, preserved sensitivity to the specific bacteriophage and pesticins. All this was evident of isolation of the spheroplasts of the plague microbe. The spheroplasts showed a capacity for pesticin production. The pesticin synthesis by the spheroplasts of the plague causative agent from the Transcaucasian Upland increased with an increase in the content of lithium chloride in the medium. The largest inhibiiton zones were observed, when 0.7-0.8 per cent of lithium chloride were present in the medium. In the spheroplasts of the plague causative agent from the Mountain Altai (the marmot variety) the pesticin synthesis was inhibited with an increase in the content of lithium chloride in the medium. The activity spectrum of the pesticins of the spheroplasts of the plague causative agent from the Transcaucasian Upland and the spheroplasts of the strains of the marmot and sandwort varieties was broader than that of the rod-like forms of these strains. The indicator properties were found in the strains of the plague microbe of the marmot and sandwort varieties with respect to the pesticins of the spheroplasts of the sel-like producing organisms and organisms from the Transcaucasian Upland.
D001688:Biological Products / Q000096:biosynthesis*; D002712:Chlorides; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D008094:Lithium / Q000378:metabolism; D012426:Russia; D013104:Spheroplasts / Q000378:metabolism* / Q000648:ultrastructure; D013997:Time Factors; D015010:Yersinia pestis / Q000254:growth & development / Q000378:metabolism*
D001688:Biological Products; D002712:Chlorides; D003470:Culture Media; D008094:Lithium
Sferoplasty shtammov chumnogo mikroba iz Zakavkazskogo nagor'ia i ikh sposobnost' k sintezu pestitsina.