[Activity of aminotransferases in the blue green alga Anacystis nidulans].
The blue-green alga Anacystis nidulans (strain L 1402-1) was grown at +37 degrees C in air (0.03 vol% CO2 and in air enriched with 3.0 vol% CO2. The effects of several inhibitors on the activity of aminotransferases, 14CO2 fixation and radioactive photosynthetic products of Anacystis were studied. No serine-pyruvate aminotransferase activity could be found in 10-2 M isonicotinyl hydrazide (INH); under the influence of this inhibitor aspartate and alanine aminotransferase were decreased about 49% respectively 17.6%. Serine-pyruvate and alanine aminotransferase activity decreased to more than 50% in 10-3 M glyoxalbisulfite. The obtained inhibitory effect of 10-4 M HPMS on serine-pyruvate aminotransferase (35%) was stronger than one the other aminotransferases. DCMU (5 x 10-6 M) inhibition on alanine aminotransferase activity was 83.7%. Under the influence of 10-3 M glyoxalbisulfite no 14C-labelled amino acids could be detected after 5 min photosynthesis; 14C-labelling of phosphoenolpyruvate, malate, phosphoglycolate and glycolic acid increased. Isonicotinyl hydrazide (10-2 M) caused in comparison to the control experiment a lower radioactivity in aspartate glutamate and phosphoenolpyruvate. The results are discussed with reference fo the operation of the glycolate pathway and a carboxylation reaction of phosphoenol-pyruvate in the blue-green alga Anacystis nidulans.
D000410:Alanine Transaminase / Q000037:antagonists & inhibitors; D001219:Aspartate Aminotransferases / Q000037:antagonists & inhibitors; D000458:Cyanobacteria / Q000201:enzymology* / Q000378:metabolism; D006016:Glycolates / Q000378:metabolism; D006037:Glyoxal / Q000494:pharmacology; D007538:Isoniazid / Q000494:pharmacology; D010728:Phosphoenolpyruvate / Q000378:metabolism; D010788:Photosynthesis; D000637:Transaminases / Q000037:antagonists & inhibitors / Q000378:metabolism*
D006016:Glycolates; D006037:Glyoxal; D010728:Phosphoenolpyruvate; D000637:Transaminases; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D007538:Isoniazid
Aminotransferase-Aktivität in der Blaualge Anacystis nidulans