Synthesis of polyadenylic acid-containing ribonucleic acid during the germination of Neurospora crassa conidia.
Ribonucleic acid (RNA) synthesized during the first 1 h of conidial germination (15 to 20, 25 to 30, and 55 to 60 min) has been characterized by sucrose-sodium dodecyl sulfate gradient centrifugation, binding to polyuridylic acid filters, and oligo(dT)-cellulose chromatography. At all labeling periods examined, polyadenylic acid-containing RNA is synthesized, processed, and incorporated into polysomes. Approximately 40% of the labeled RNA sedimenting between 5 and 17S binds to polyuridylic acid filters. RNA which binds to oligo(dT)-cellulose displays a heterogeneous distribution in sucrose-sodium dodecyl sulfate gradients with a major, broad peak at 10-16S. In addition, some polyadenylic acid-containing RNA sediments beyond the 25S marker. Approximately 3% of the [3H]adenosine in pulse-labeled polysomal RNA is in polyadenylic acid segments resistant to pancreatic and T1 ribonucleases.
D009491:Neurospora / Q000378:metabolism*; D009492:Neurospora crassa / Q000032:analysis / Q000254:growth & development / Q000378:metabolism*; D011061:Poly A / Q000032:analysis; D011132:Polyribosomes / Q000378:metabolism; D012313:RNA / Q000032:analysis / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis*; D012335:RNA, Ribosomal / Q000032:analysis / Q000096:biosynthesis; D012260:Ribonucleases / Q000378:metabolism; D013172:Spores, Fungal / Q000032:analysis / Q000254:growth & development / Q000378:metabolism
D012333:RNA, Messenger; D012335:RNA, Ribosomal; D011061:Poly A; D012313:RNA; D012260:Ribonucleases