The cleavage of transfer RNA by a single strang specific endonuclease from Neurospora crassa.
Endonuclease from Neurospora crassa (NcNase), an enzyme with specificity for polynucleotides lacking an ordered structure, was shown to cleave su+3 tRNATyr from E. coli preferentially in the anticodon region. The enzyme cleaved unfractionated tRNA primarily to 30 - 50 nucleotide size fragments, implying that most or all tRNA species are also cleaved in the anticodon region. The 3' terminal sequence C-A was cleaved as well. The results are discussed with respect to the three dimensional structure of tRNA.
D000926:Anticodon; D001345:Autoradiography; D003090:Coliphages / Q000201:enzymology; D004590:Electrophoresis, Paper; D004591:Electrophoresis, Polyacrylamide Gel; D004720:Endonucleases; D004926:Escherichia coli; D007700:Kinetics; D009491:Neurospora / Q000201:enzymology*; D009492:Neurospora crassa / Q000201:enzymology*; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000302:isolation & purification; D011119:Polynucleotides / Q000302:isolation & purification; D012343:RNA, Transfer; D012260:Ribonucleases
D000926:Anticodon; D009841:Oligonucleotides; D011119:Polynucleotides; D012343:RNA, Transfer; D004720:Endonucleases; D012260:Ribonucleases