[Study of the conformational changes of serum albumin molecules within a pre-denaturation temperature range by a spin probe technique].
To study conformational changes of protein molecules in a pre-denaturation temperature range, nitroxyl radicals adsorbed by a protein are suggested to be used. A spin probe technique is specially developed to be implied for this aim by using a probe specifically bound to the bovine serum albumin molecule, it was possible to reveal a dependence of the rotation correlation time of the adsorbed radical and the environment polarity on the temperature. The data obtained testify in favour of a change occurring in the intramolecular structure of the protein under study due to a temperature change within the pre-denaturation temperature range.
D000818:Animals; D001665:Binding Sites; D002417:Cattle; D004578:Electron Spin Resonance Spectroscopy; D008433:Mathematics; D011485:Protein Binding; D011487:Protein Conformation; D012710:Serum Albumin, Bovine; D013113:Spin Labels
D013113:Spin Labels; D012710:Serum Albumin, Bovine
Issledovanie konformatsionnykh izmeneniĭ molekul syvorotochnogo al'bumina v preddenaturatsionnom intervale temperatur metodom spinovogo zonda