The distribution and properties of aspartyl transfer RNA in human and animal tumors.
The elution profile of aspartyl transfer RNA (aspartyl-tRNA) from reversed phase 5 chromatography for tRNA from a spectrum of animal tissues and tumors and human tumors has been examined. It was found that SV40-induced hamster tumors, BHK21/cl 13 cells in culture, certain carcinogen-induced tumors in the Ehrlich ascites tumor, and a number of human carcinomas and adenocarcinomas contained a distinct increase (3- to 20-fold) in the percentage of a late-eluting aspartyl-tRNA over that found in nonmalignant tissues, other animal tumors, and in human melanomas and sarcomas. The ability of the late-eluting aspartyl-tRNAAspIV to bind to ribosomes in the presence of the codons for aspartic acid was compared to that of aspartyl-tRNAAspIII and was found to be approximately the same. Also, the ability of each of the 4 isoaccepting species of aspartyl-tRNA to engage in ribosomal incorporation of aspartic acid into a polypeptide was determined. All 4 isoacceptors function equally well in the amino acid incorporation.
D000230:Adenocarcinoma / Q000378:metabolism; D000818:Animals; D001224:Aspartic Acid / Q000378:metabolism; D002277:Carcinoma / Q000378:metabolism; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism; D002478:Cells, Cultured; D006224:Cricetinae; D005260:Female; D005815:Genetic Code; D006801:Humans; D008297:Male; D008545:Melanoma / Q000378:metabolism; D051379:Mice; D009369:Neoplasms / Q000378:metabolism*; D009374:Neoplasms, Experimental / Q000378:metabolism; D012334:RNA, Neoplasm / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism*; D051381:Rats; D012270:Ribosomes / Q000378:metabolism; D012509:Sarcoma / Q000378:metabolism; D013539:Simian virus 40
D012334:RNA, Neoplasm; D001224:Aspartic Acid; D012343:RNA, Transfer