Immunofluorescence and anti-complement immunofluorescence absorption tests for quantitation of Epstein-Barr virus-associated antigens.
Immunofluorescence absorption methods are described which permit quantitative estimation and differentiation of Epstein-Barr virus (EBV)-associated antigens (virus capsid antigen, VCA, early antigen, EA and EBV-determined nuclear antigen, EBNA) in cell extracts. EBNA was present in all cell lines (producer and non-producer) which carried the EBV-genome, while VCA and EA were present in producer lines only. All the antigens were absent from a lymphoid cell line (MOLT-4) which lacked the EBV-genome, as well as from leukemia cells from peripheral blood. The techniques demonstrated antigenic identity of the various antigens when prepared from different cell lines.
D000042:Absorption; D000818:Animals; D000906:Antibodies; D000937:Antigen-Antibody Reactions; D000951:Antigens, Neoplasm; D000956:Antigens, Viral / Q000032:analysis*; D002051:Burkitt Lymphoma / Q000276:immunology; D002417:Cattle; D002460:Cell Line; D003165:Complement System Proteins; D005455:Fluorescent Antibody Technique; D004854:Herpesvirus 4, Human / Q000276:immunology*; D007106:Immune Sera; D007945:Leukemia, Lymphoid / Q000276:immunology; D008221:Lymphoid Tissue
D000906:Antibodies; D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D007106:Immune Sera; D003165:Complement System Proteins