[Comparative investigations of quantitative determination of cell-mediated immunity by skin test, macrophage migration inhibition test, and lymphocyte transformation test in guinea pigs (author's transl)].
Following an intradermal immunization of guinea pigs with bovine gamma globulin (BGG) and/or human serum albumin (HSA) emulsified in complete Freund's adjuvant we studied the cell-mediated immune response and its manipulation by 6-mercaptopurine. Three accepted correlates of cell-mediated immunity (CMI) have been used for the estimation of the immune reaction: - Intracutaneous test (IT) - Macrophage migration inhibition test (MMIT) - Lymphocyte transformation test (LTT). Comparative studies of quantitative determinations of CMI showed a correlation of the results of the in vitro test systems (MMIT and LTT) with the state of delayed hypersensitivity of the experimental animals, estimated by the IT in vivo. The cell-mediated immune response against BGG was much higher than that against HSA. This could be shown by all three test systems. We detected an immunosuppressive influence of 6-mercaptopurine on the CMI of guinea pigs, due to the dose and the mode of application. Thereby the in vitro test systems detected quantitative differences and indicated already a partial success of the immunosuppressive therapy, whereas the IT allowed a qualitative estimation only.
D000818:Animals; D002464:Cell Migration Inhibition; D006168:Guinea Pigs; D007111:Immunity, Cellular / Q000187:drug effects; D007165:Immunosuppression Therapy; D007428:Intradermal Tests; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology; D015122:Mercaptopurine / Q000494:pharmacology
D015122:Mercaptopurine
Vergleichende Untersuchungen zur quantitativen Bestimmung der zellvermittelten Immunität durch Intrakutantest, Makrophagenmigrationshemmtest und Lymphozytentransformationstest am Meerschweinchen.