title: Failure of attenuated temperature-sensitive influenza A (H3N2) virus to induce heterologous interference in humans to parainfluenza type 1 virus.
abstract: The present investigation was undertaken to determine if a candidate live vaccine virus, influenza A/Hong Kong/68-ts-1 [E] (H3N2), induced heterologous interference against an interferon-sensitive, wild-type, parainfluenza type 1 challenge virus. The parainfluenza virus was administered 7 days after Hong Kong/68-ts-1 [E] virus infection. The clinical response, daily quantitative virus shedding, interferon production, and serum and nasal wash antibody responses were determined in an experimental group (influenza A virus followed by parainfluenza virus) and 10 volunteers in a control group (parainfluenza virus only). The volunteers were selected on the basis of susceptibility to the two viruses, i.e. serum hemagglutination-inhibition antibody titer of is less than or greater to 1:8 for influenza virus and low nasal wash antibody titer (is less than or greater to 1:8) for parainfluenza virus. Despite a 100% infection rate in the Hong Kong/68-ts-1 [E] vaccinees, no heterologous interference was induced against the parainfluenza type 1 virus challenge.
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D046508:Culture Techniques; D005347:Fibroblasts; D006382:Hemadsorption; D006801:Humans; D053122:Influenza A Virus, H3N2 Subtype; D009980:Influenza A virus / Q000276:immunology*; D007251:Influenza, Human / Q000276:immunology*; D007372:Interferons / Q000096:biosynthesis; D007668:Kidney; D008253:Macaca mulatta; D009093:Mucus / Q000276:immunology; D009297:Nasal Mucosa; D009500:Neutralization Tests; D009975:Orthomyxoviridae / Q000276:immunology*; D010222:Parainfluenza Virus 1, Human / Q000276:immunology*; D018184:Paramyxoviridae Infections / Q000276:immunology*; D012845:Sindbis Virus / Q000276:immunology; D014762:Viral Interference; D010948:Viral Plaque Assay; D014765:Viral Vaccines
chemical_list: D000914:Antibodies, Viral; D014765:Viral Vaccines; D007372:Interferons