Fractionation and characterization of euchromatin isolated from mouse ascites sarcoma cells.
Euchromatin specimen prepared by the usual method formed large clumps and had various shapes under electron microscopy. A method of separation of the euchromatin specimen into chromatin fractions having relatively homogeneous form was examined and partial characterization of these fractions was carried out. The heavy euchromatin fraction was a large network of thin fibrils (about 100 A in diameter) and various thick fibers. The intermediate euchromatin fraction consisted of relatively homogeneous networks of thick knobby fibers (about 250 A in diameter). The light euchromatin fraction had metworks of thick fibers. These chromatin fractions were quantitatively prepared from sonicated nuclei of mouse ascites sarcoma cells. Twenty-one or twenty-two bands of non-histone proteins besides histones were detected in these chromatin fractions by SDS-polyacrylamide gel electrophoresis. There were significant differences in the electrophoretic patterns of non-histone proteins among these chromatin fractions.
D000818:Animals; D002458:Cell Fractionation; D002843:Chromatin / Q000032:analysis* / Q000648:ultrastructure; D004273:DNA, Neoplasm / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000032:analysis; D051379:Mice; D012334:RNA, Neoplasm / Q000032:analysis; D012513:Sarcoma, Experimental / Q000032:analysis* / Q000648:ultrastructure
D002843:Chromatin; D004273:DNA, Neoplasm; D006657:Histones; D012334:RNA, Neoplasm