title: A specific cyclic guanosine 3':5'-monophosphate-binding protein in Caulobacter crescentus.
abstract: A binding protein specific for cyclic guanosine 3':5'-monophosphate (cyclic GMP) has been partially purified from extracts of the eubacterium Caulobacter crescentus and resolved from cyclic adenosine 3':5'-monophosphate (cyclic AMP)-binding activity. Binding of cyclic GMP is not affected by the addition of cyclic AMP or 5'-GMP, but is inhibited about 50 percent by a 50-fold molar excess of dibutyryl cyclic GMP or cyclic hypoxanthine 3':5'-monophosphate. The apparent dissociation constant for the cyclic GMP-binding protein complex is 1.1 X 10(-6) M.
mesh_terms: D001426:Bacterial Proteins / Q000302:isolation & purification / Q000378:metabolism; D001665:Binding Sites; D001667:Binding, Competitive; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D002918:Chymotrypsin; D000242:Cyclic AMP / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism*; D003851:Deoxyribonucleases; D005051:Eubacterium / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D009712:Nucleotides, Cyclic / Q000494:pharmacology; D011402:Pronase; D011485:Protein Binding; D011955:Receptors, Drug; D012260:Ribonucleases; D014357:Trypsin
chemical_list: D001426:Bacterial Proteins; D009712:Nucleotides, Cyclic; D011955:Receptors, Drug; D000242:Cyclic AMP; D003851:Deoxyribonucleases; D012260:Ribonucleases; D002918:Chymotrypsin; D014357:Trypsin; D011402:Pronase; D006152:Cyclic GMP