Cholesterol and beta-lipoprotein on lipid inclusions and lysosomal and mitochondrial permeability of cultured heart muscle and endothelioid cells.
Cultured rat heart endothelioid cells were used to study the effect of beta-lipoprotein and cholesterol on the production and persistence of cellular lipid inclusions. Both heart endothelioid and muscle cells were used to study the effects of beta-lipoprotein with and without cholesterol or cholesterol esters on lysosomal or mitochondrial lability. Beta lipoprotein enhanced the production and maintanence of lipid inclusions. In the concentration employed (20mg%) it alone produced no effect after 72 hours on lysosomal or mitochondrial lability but with cholesterol, cholesterol stearate, cholesterol oleate or cholesterol linoleate (5mg%) there was a trend toward labilization.
D000818:Animals; D002478:Cells, Cultured; D002784:Cholesterol / Q000494:pharmacology*; D002788:Cholesterol Esters / Q000494:pharmacology; D004727:Endothelium / Q000378:metabolism; D006801:Humans; D050356:Lipid Metabolism; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D008247:Lysosomes / Q000378:metabolism*; D008931:Mitochondria, Muscle / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism* / Q000648:ultrastructure; D010539:Permeability; D051381:Rats
D002788:Cholesterol Esters; D008077:Lipoproteins, LDL; D002784:Cholesterol