Influence of riboflavin on disturbances in trytophan metabolism and hepatoma production after a single dose of aflatoxin B1.
Female Wistar rats were given a single oral dose of aflatoxin B1, either alone or with a large amount of riboflavin. Biochemical and histologic studies were performed for 30 months. Nine animals of 19 in the aflatoxin-treated group and only 5 of 18 in the riboflavin-aflatoxin-treated group developed hepatomas. The number of rats was insufficient for tests of statistical significance to be fruitful. Urinary excretion of tryptophan metabolites was studied in aflatoxin- and riboflavin-treated rats after an oral administration of 10 mg tryptophan/100 g rat. Riboflavin did not affect the percentage of aflatoxin-treated animals with abnormal urinary excretion patterns, but did increase the magnitude of the disturbances in elimination of kynurenic and xanthurenic acids. The hepatic tryptophan-oxygenase activity was increased only in the two groups given riboflavin, and the levels of nucleic acids were the same in all groups.
D000348:Aflatoxins / Q000494:pharmacology / Q000633:toxicity; D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000139:chemically induced*; D005260:Female; D007736:Kynurenic Acid / Q000652:urine; D008099:Liver / Q000378:metabolism; D008113:Liver Neoplasms / Q000139:chemically induced*; D009374:Neoplasms, Experimental / Q000139:chemically induced; D051381:Rats; D012256:Riboflavin / Q000494:pharmacology*; D014364:Tryptophan / Q000378:metabolism* / Q000652:urine; D014366:Tryptophan Oxygenase / Q000378:metabolism; D014977:Xanthurenates / Q000652:urine
D000348:Aflatoxins; D014977:Xanthurenates; D014364:Tryptophan; D014366:Tryptophan Oxygenase; D007736:Kynurenic Acid; D012256:Riboflavin