title: Isolation and characterization of 'dense particles' from poliovirus-infected HeLa cells.
abstract: Dense poliovirus particles (buoyant density 1.44 g/ml in CsCl) isolated from infected HeLa cells contain the normal four structural polypeptides VP1 to VP4, and 35S poliovirus RNA. In addition, small amounts of VPo and single-stranded RNA sedimenting slower than the poliovirus genome are present. Dense particles have a low specific infectivity, are neutralized by type-specific poliovirus antisera, and are detected during growth as early as normal virus but disappear when virus production stops.  They appear to represent a different, more open, conformation of the normal virus capsid.
mesh_terms: D000596:Amino Acids; D002499:Centrifugation, Density Gradient; D002725:Chloroform / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells; D006801:Humans; D007181:Inclusion Bodies, Viral / Q000187:drug effects; D008623:Mercaptoethanol / Q000494:pharmacology; D009500:Neutralization Tests; D010455:Peptides / Q000032:analysis; D017955:Poliovirus / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification; D011092:Polyethylene Glycols / Q000494:pharmacology; D012367:RNA, Viral / Q000032:analysis; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D014508:Urea / Q000494:pharmacology; D014529:Uridine; D014764:Viral Proteins / Q000032:analysis
chemical_list: D000596:Amino Acids; D010455:Peptides; D012367:RNA, Viral; D014764:Viral Proteins; D012967:Sodium Dodecyl Sulfate; D011092:Polyethylene Glycols; D008623:Mercaptoethanol; D002725:Chloroform; D014508:Urea; D014529:Uridine