The role of acidic proteins from cytoplasmic fractions of Krebs II ascites cells for efficient translation.
Acidic proteins with affinity to RNA from cytoplasmic fraction of Krebs II Ascites cells were isolated by means of affinity chromatography on RNA-Sepharose CN-Br-columns. Stepwise elution with 350 mM [K+] and 1000 mM [K+] removed two fractions of proteins both of which are required for the formation of 40S- or 80S-initiation complexes and for efficient translation.
D000818:Animals; D002287:Carcinoma, Krebs 2 / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D004273:DNA, Neoplasm / Q000378:metabolism; D000882:Haplorhini; D006367:HeLa Cells / Q000378:metabolism; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D010442:Peptide Chain Initiation, Translational; D010445:Peptide Elongation Factors / Q000302:isolation & purification / Q000378:metabolism*; D010448:Peptide Initiation Factors / Q000302:isolation & purification / Q000378:metabolism*; D011132:Polyribosomes / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism
D004273:DNA, Neoplasm; D010445:Peptide Elongation Factors; D010448:Peptide Initiation Factors; D012333:RNA, Messenger