title: Zonal changes in renal structure and phospholipid metabolism in potassium-deficient rats.
abstract: Morphologic alterations and membrane metabolism were studied in the kidneys of rats fed a low potassium diet. Transmission and scanning electron microscopy following perfusion-fixation of kidneys revealed that the earliest morphologic change occurs in cells of the papillary tip in which multivesicular bodies, a specific type to lysosome, appear after 1 day. Increased depletion leads to extension of the lesion to all cells of the papilla. After 1 week, a narrow band of hyperplasia in the inner red medulla appears; this band is characterized by adenomatous proliferation of intercalated and light cells and partial obstruction of collecting tubules. These alterations and cortical growth in the normal pattern result in increased renal weight. New membrane formation for lysosomes and growing cells was studied by measuring the rate of [14C]choline incorporation into phospholipid in slices from five zones of the kidney. In the papilla the rate increased 39 per cent after 18 hours, the earliest change detected. After 36 hours the rate increased in inner red medulla by 28 per cent, inner cortex by 25 per cent and outer cortex by 40 per cent. [14C]choline was a specific precursor of the three renal phospholipids, phosphatidylcholine, lysophosphatidylcholine, and sphingomyelin. The relative distribution of the label did not change with growth induced by potassium depletion. The results indicate that potassium depletion induces early increases in the formation of cell membrane phospholipid which correlate with specific morphologic changes in different zones within the kidney.
mesh_terms: D000818:Animals; D002455:Cell Division; D002462:Cell Membrane / Q000378:metabolism; D002794:Choline / Q000378:metabolism; D006965:Hyperplasia / Q000378:metabolism; D007008:Hypokalemia / Q000378:metabolism / Q000473:pathology*; D007668:Kidney / Q000473:pathology*; D007672:Kidney Cortex / Q000378:metabolism / Q000473:pathology*; D007674:Kidney Diseases / Q000473:pathology; D007679:Kidney Medulla / Q000378:metabolism / Q000473:pathology*; D007682:Kidney Pelvis / Q000473:pathology; D007684:Kidney Tubules / Q000473:pathology; D008247:Lysosomes / Q000378:metabolism; D008297:Male; D008855:Microscopy, Electron, Scanning; D009929:Organ Size; D010504:Periodic Acid / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*; D051381:Rats
chemical_list: D010743:Phospholipids; D010504:Periodic Acid; D002794:Choline