title: Microbiological transformations. XI. The use of immobilized Rhodotorula mucilaginosa cells to reduce some ketones.
abstract: Rhodotorula mucilaginosa cells were immobilized by polymerizing acrylamide with the addition of a cross-linked agent, a catalyst and an initiator. This gel was used for the stereospecific reduction of acetophenone and alpha-acetylnaphthalene resulting chromatographically pure alcohols with absolute configuration S. When karen-3-dion-2,5 was used as substrate, four products were obtained. Androstenolone was reduced with 30% yield. Activity of the immobilized cells could be regenerated by glucose.
mesh_terms: D000098:Acetophenones / Q000378:metabolism; D003512:Cyclohexanones / Q000378:metabolism; D003687:Dehydroepiandrosterone / Q000378:metabolism; D004800:Enzymes, Immobilized; D007659:Ketones / Q000378:metabolism*; D003904:Mitosporic Fungi / Q000201:enzymology*; D012248:Rhodotorula / Q000201:enzymology*; D013729:Terpenes / Q000378:metabolism
chemical_list: D000098:Acetophenones; D003512:Cyclohexanones; D004800:Enzymes, Immobilized; D007659:Ketones; D013729:Terpenes; D003687:Dehydroepiandrosterone