Beef-heart submitochondrial particles: a mixture of mitochondrial inner and outer membranes.
1. EPR spectra at 9 GHz and 83 degrees K of NADH-reduced anaerobic beef-heart submitochondrial particles, prepared from mitochondria by sonication and centrifugation, contain a signal (gz equals to 2.01, gy equals to 1.94, gx equals to 1.89) due to an iron-sulphur center of the mitochondrial outer membrane. 2. The ratio of inner and outer membranes in submitochondrial particles is not greatly different from that in beef-heart mitochondria. 3. Beef-heart submitochondrial particles free from outer-membrane contamination have been prepared by free-flow electrophoresis. EPR spectra at 83 degrees K of such particles are presented.
D000255:Adenosine Triphosphate; D000693:Anaerobiosis; D000818:Animals; D002417:Cattle; D004578:Electron Spin Resonance Spectroscopy; D007501:Iron / Q000032:analysis; D008274:Magnesium; D008566:Membranes / Q000648:ultrastructure; D008667:Metalloproteins / Q000032:analysis; D008928:Mitochondria / Q000032:analysis; D008931:Mitochondria, Muscle / Q000648:ultrastructure*; D009206:Myocardium; D009243:NAD; D010084:Oxidation-Reduction; D013010:Sonication; D013455:Sulfur / Q000032:analysis
D008667:Metalloproteins; D009243:NAD; D013455:Sulfur; D000255:Adenosine Triphosphate; D007501:Iron; D008274:Magnesium