Induction of DNA polymerase in polyoma virus-infected mouse cells requires transcription and translation.
The induction of DNA polymerase activity and of DNA synthesis in polyoma virus-infected mouse kidney cells is inhibited by actinomycin D and cycloheximide, even in cells that are already T-antigen positive. This indicates that the induction of the DNA-synthesizing apparatus requires transcription and translation. The increase in cellular DNA polymerase activity and in the DNA synthesizing capacity follows the appearance of T-antigen only after a characteristic lag period of several hours.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D046508:Culture Techniques; D003513:Cycloheximide / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D004254:DNA Nucleotidyltransferases / Q000378:metabolism*; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis; D003609:Dactinomycin / Q000494:pharmacology; D004790:Enzyme Induction; D051379:Mice; D011120:Polyomavirus / Q000254:growth & development* / Q000276:immunology / Q000378:metabolism; D014176:Protein Biosynthesis; D013696:Temperature; D014158:Transcription, Genetic
D000956:Antigens, Viral; D004279:DNA, Viral; D003609:Dactinomycin; D004247:DNA; D003513:Cycloheximide; D004254:DNA Nucleotidyltransferases