title: Purification of the arom multienzyme aggregate from Euglena gracilis.
abstract: The arom multienzyme complex that catalyzes steps two through six in the prechorismate polyaromatic amino acid biosynthetic pathway has been purified up to 2000-fold from Euglena gracilis. The native arom aggregate has a molecular weight of approx. 249 000 based on a sedimentation coefficient of 9.5 and Stokes radius of 60 angstrom. A comparison between the arom aggregates of Neurospora crassa and Euglena gracilis and the possible phylogenetic relationships between the organisms are discussed.
mesh_terms: D000596:Amino Acids / Q000096:biosynthesis*; D000818:Animals; D002827:Chorismic Acid / Q000096:biosynthesis; D002845:Chromatography; D004798:Enzymes / Q000302:isolation & purification; D005056:Euglena gracilis / Q000378:metabolism*; D008970:Molecular Weight; D009492:Neurospora crassa / Q000201:enzymology; D013045:Species Specificity
chemical_list: D000596:Amino Acids; D004798:Enzymes; D002827:Chorismic Acid