High volume screening procedures for hypobetalipoproteinemic activity in rats.
We describe high volume screening tests for hypobetalipoproteinemic agents in which compounds are administered orally to cholesterol-cholic acid fed (hypercholesterolemic( or normally fed weanling rats for 4 days. In these tests total serum cholesterol levels and heparin precipitating lipoproteins (HPL) are determined by automated analyses interfaced with a computer which eliminates all manual data reduction and provides necessary reports. The hypercholesterolemic rat test detects compounds which specifically reduce HPL (beta and pre beta lipoproteins) causing a decrease in the HPL: cholesterol ratio. Such activity is called hypobetalipoproteinemia. This activity is exhibited by bicyclo (2.2.2)-octyloxyaniline (U-26328) but not by any of the familiar hypocholesterolemic agents including clofibrats, lifibrats, nicotinic acid, probucol, triparanol, lentysine, D-throxine or the estrogens estrone and diethylstilbestrol.
D000703:Analog-Digital Conversion; D000818:Animals; D002784:Cholesterol / Q000097:blood*; D002791:Cholesterol, Dietary; D002793:Cholic Acids / Q000494:pharmacology; D004036:Diet, Atherogenic; D005260:Female; D006937:Hypercholesterolemia / Q000097:blood*; D008077:Lipoproteins, LDL / Q000037:antagonists & inhibitors / Q000097:blood*; D008297:Male; D010600:Pharmacology; D051381:Rats; D012107:Research Design; D012737:Sex Factors
D002791:Cholesterol, Dietary; D002793:Cholic Acids; D008077:Lipoproteins, LDL; D002784:Cholesterol