Early cytoplasmic vacuolization of African green monkey kidney cells by SV40.
As early as 3--4 hours after infection with SV40 at a high input multiplicity, African green monkey (Cercopithecus aethiops) kidney (AGMK) cells developed cytoplasmic vacuolization. At 10--20 hours after infection, the vacuolization reached its maximal level, then disappeared and SV40 specific cytopathic change followed. This vacuolization developed before the synthesis of the specific T and V antigens. This early cytoplasmic vacuolization (ECV) was prevented by preincubating the virus with specific antiserum, or by heating the virus with MgCl2. The ECV could be induced by UV-irradiated SV40. Addition of metabolic inhibitors had no effect on the induction of the ECV. These results suggest that the capacity to induce the ECV resides in a structural component(s) of SV40 virion and the vacuolization is not associated with the replication of SV40.
D000818:Animals; D000914:Antibodies, Viral; D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D002467:Cell Nucleus / Q000276:immunology / Q000648:ultrastructure; D002712:Chlorides; D002522:Chlorocebus aethiops; D003513:Cycloheximide / Q000494:pharmacology; D003561:Cytarabine / Q000494:pharmacology; D003588:Cytopathogenic Effect, Viral; D003593:Cytoplasm / Q000648:ultrastructure; D003609:Dactinomycin / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D006358:Hot Temperature; D002479:Inclusion Bodies / Q000648:ultrastructure*; D007668:Kidney; D008274:Magnesium / Q000494:pharmacology; D011830:Radiation Effects; D013539:Simian virus 40 / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D013997:Time Factors; D014466:Ultraviolet Rays; D014617:Vacuoles / Q000187:drug effects / Q000648:ultrastructure*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D002712:Chlorides; D003561:Cytarabine; D003609:Dactinomycin; D003513:Cycloheximide; D008274:Magnesium