Thermolabile T (tumor) antigen from cells transformed by a temperature-sensitive mutant of simian virus 40.
Partially purified tumor (T) antigen from a strain of Chinese hamster lung cells transformed by wild-type simian virus 40 (SV 40) and either of two temperature-sensitive SV 40 mutants has been studied as a DNA binding protein. The DNA binding activity present in the T-antigen-containing fractions is inhibited by purified hamster anti-T IgG but not by equivalent amounts of nonimmune hamster IgG. T from either wild-type- or tsC219-transformed cells is relatively stable during heating at 44 degrees compared to T prepared from tsA239-transformed cells. These results strongly suggest that T is a product of the SV 40 A gene.
D000951:Antigens, Neoplasm / Q000302:isolation & purification; D000956:Antigens, Viral / Q000302:isolation & purification; D001665:Binding Sites; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002874:Chromosome Mapping; D004279:DNA, Viral / Q000378:metabolism; D009154:Mutation; D013539:Simian virus 40 / Q000276:immunology* / Q000378:metabolism*; D013696:Temperature
D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D004279:DNA, Viral