title: Comparison of the characteristics of avian reoviruses isolated from the digestive and respiratory tract, with viruses isolated from the synovia.
abstract: Two-week-old gnotobiotic chicks were inoculated in the foot pad with viruses isolated from synovia and synovial membrane-WVU 1464-29H, WVU 1675, WVU 2937, WVU 2986, and WVU 71-212; from digestive tract-reoviruses 24, 25, and 59; or from respiratory tract-reovirus Fahey-Crawley (FC). All viruses induced swelling of the foot pad and inflammatory changes of synovial membrane. Serum from virus-infected chicks had a common agar gel precipitin (AGP) line. On the basis of the plaque-reduction test in primary chicken kidney (PCK) cells, the viruses were classified into 4 major serotypes. All viruses produced cytopathic effects (CPE) in primary chicken tissue cultures. Other than reovirus FC and WVU 1464-29H, all viruses produced CPE in the Vero cell line.
mesh_terms: D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D003588:Cytopathogenic Effect, Viral; D004064:Digestive System / Q000382:microbiology*; D005779:Immunodiffusion; D009500:Neutralization Tests; D018165:Orthoreovirus / Q000276:immunology / Q000302:isolation & purification* / Q000472:pathogenicity; D011201:Poultry Diseases / Q000209:etiology / Q000276:immunology; D011212:Poxviridae / Q000302:isolation & purification*; D012087:Reoviridae / Q000276:immunology / Q000302:isolation & purification* / Q000472:pathogenicity; D012137:Respiratory System / Q000382:microbiology*; D013583:Synovial Membrane / Q000382:microbiology*; D013585:Synovitis / Q000209:etiology / Q000276:immunology / Q000662:veterinary; D010948:Viral Plaque Assay