'Freezing' of the calcium-regulated structures of gizzard thin filaments by glutaraldehyde.
Reconstituted thin filaments (the actin-tropomyosin-leiotonin complex) of chicken gizzard were cross-linked with glutaraldehyde either in the presence or absence of Ca2+. The ability of resultant thin filaments to activate myosin ATPase was 'frozen' in the activated or inactivated state, respectively. This result clearly indicates the existence of actin-linked regulation in smooth muscle.
D000199:Actins / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000447:Aldehydes / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002645:Chickens; D005895:Gizzard, Avian; D005976:Glutaral / Q000378:metabolism*; D009124:Muscle Proteins / Q000378:metabolism*; D014335:Tropomyosin / Q000378:metabolism
D000199:Actins; D000447:Aldehydes; D009124:Muscle Proteins; D014335:Tropomyosin; D000251:Adenosine Triphosphatases; D002118:Calcium; D005976:Glutaral