A new method for the preparation of beta-amylase from sweet potato.
A simple method for the preparation of sweet potato beta-amylase by thymol amylose adsorption is described. The method is far more efficient and gives higher recovery of the enzyme. The crystalline enzyme thus obtained is found to be homogeneous by gel chromatography, polyacrylamide gel electrophoresis.
D000327:Adsorption; D000645:Ammonium Sulfate; D000681:Amylases / Q000302:isolation & purification*; D000688:Amylose / Q000031:analogs & derivatives; D002850:Chromatography, Gel; D003460:Crystallization; D004591:Electrophoresis, Polyacrylamide Gel; D005590:Fractional Precipitation; D008722:Methods; D008970:Molecular Weight; D010944:Plants / Q000201:enzymology*; D013943:Thymol / Q000031:analogs & derivatives; D001614:beta-Amylase / Q000302:isolation & purification*
D013943:Thymol; C019576:thymol amylose; D000688:Amylose; D000681:Amylases; D001614:beta-Amylase; D000645:Ammonium Sulfate