Early stimulation of human chorionic gonadotropin secretion by dibutyryl cyclic AMP and theophylline in human malignant trophoblast cells in vitro: inhibition by actinomycin D, alpha-amanitin, and cordycepin.
Human malignant trophoblast cells (BeWo line) in culture were employed to investigate the early stimulation of human chorionic gonadotropin (hCG) secretion by 1 mM dibutyryl cyclic AMP and 1 mM theophylline (dbT). The earliest increase in secreted immunoreactive hCG occurred at 3 1/2 h following addition of dbT, and was preceded by an increase in intracellular hCG. These results suggested that dbT stimulated hCG synthesis, rather than release. Addition of either 0.062 mug/ml actinomycin D or 100 mug/ml cordycepin along with dbT, or 3 mug/ml alpha-amanitin 9 h prior to addition of dbT, prevented the increase in hCG secretion at 3 1/2-8 h. It was concluded that synthesis of RNA (possibly messenger RNA) is required for the early stimulation of hCG secretion by dbT.
D000546:Amanitins / Q000494:pharmacology*; D003994:Bucladesine / Q000494:pharmacology*; D002460:Cell Line; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000378:metabolism*; D003609:Dactinomycin / Q000494:pharmacology*; D003839:Deoxyadenosines / Q000494:pharmacology*; D005260:Female; D006801:Humans; D011247:Pregnancy; D012334:RNA, Neoplasm / Q000096:biosynthesis; D013806:Theophylline / Q000494:pharmacology*; D014328:Trophoblastic Neoplasms / Q000378:metabolism; D014327:Trophoblasts / Q000378:metabolism*
D000546:Amanitins; D006063:Chorionic Gonadotropin; D003839:Deoxyadenosines; D012334:RNA, Neoplasm; D003609:Dactinomycin; D003994:Bucladesine; D013806:Theophylline