[Glucoamylase immobilized on AE-cellulose].
Glucoamylase from Endomycopsis species 20-9 was immobilized on AE-cellulose by covalent binding with the use of glutaraldehyde. The activity of immobilized preparations was measured as a function of pH, buffer concentration, temperature and immobilization time. The most active preparations of immobilized glucoamylase were obtained in 0.2 M Na-phosphate buffer, pH 5.0--7.0 at 4--20 degrees C for 4--24 hrs. In the presence of substrate--starch the activity of immobilized glucoamylase increased almost two-fold whereas glucose had no effect of this kind.
D000681:Amylases / Q000378:metabolism; D002482:Cellulose / Q000031:analogs & derivatives; D004800:Enzymes, Immobilized; D005947:Glucose; D005976:Glutaral; D004718:Saccharomycetales / Q000201:enzymology; D013213:Starch; D001614:beta-Amylase / Q000378:metabolism
D004800:Enzymes, Immobilized; D002482:Cellulose; D013213:Starch; C010021:aminoethyl cellulose; D000681:Amylases; D001614:beta-Amylase; D005947:Glucose; D005976:Glutaral
Gliukoamilaza, immobilizovannaia na AE-tselliuloze.