Substrate specificity of lecithin:cholesterol acyltransferase. Esterification of desmosterol, b-sitosterol, and cholecalciferol in human plasma.
Desmosterol and beta-sitosterol were esterified when incubated with normal human plasma. The initial rate of demosterol esterification was 1.7 times faster, and that of beta-sitosterol 0.4 times slower, than the esterification rate of cholesterol. These ratios were found to be almost the same when plasma from different normal individuals was tested. Plasma from a patient with familial lecithin:cholesterol acyltransferase deficiency did not esterify any of the sterols. The esterification of desmosterol and beta-sitosterol was considerably slower in normal plasma in which in vitro cholesterol esterification previously had taken place. The different esterification rates could not be explained by a different affinity of the plasma lipoproteins for the sterols tested. Cholecalciferol added to normal plasma did not become esterified.
D000217:Acyltransferases / Q000097:blood*; D000328:Adult; D002762:Cholecalciferol / Q000097:blood*; D002784:Cholesterol / Q000097:blood; D003897:Desmosterol / Q000097:blood*; D004951:Esterification; D005260:Female; D006801:Humans; D007863:Lecithin Cholesterol Acyltransferase Deficiency; D008055:Lipids / Q000097:blood; D008074:Lipoproteins / Q000097:blood; D008297:Male; D007862:Phosphatidylcholine-Sterol O-Acyltransferase / Q000097:blood*; D012855:Sitosterols / Q000097:blood*; D013997:Time Factors
D008055:Lipids; D008074:Lipoproteins; D012855:Sitosterols; D002762:Cholecalciferol; D003897:Desmosterol; D002784:Cholesterol; D000217:Acyltransferases; D007862:Phosphatidylcholine-Sterol O-Acyltransferase