Preparation of carcinoembryonic antigen (CEA) containing significantly increased amounts of galactose and galactosamine.
CEA was prepared by combined isoelectric precipitation, ultrafiltration and column chromatography under controlled conditions of pH. The resulting immunologically active materials were higher in carbohydrate (85-87%), N-acetyl galactosamine (10-11.5%) and galactose (28-32%) content than that previously reported. Differences in amino acid yield were also noted; the concentrations of aspartate, serine, glycine and alanine being higher and that of lysine, histidine, arginine, proline, valine, isoleucine, leucine and tyrosine were lower than that reported for CEA prepared by previous methods. The tumor tissues for CEA extraction were obtained from two Group O Rh positive deceased. Neither preparation showed Group A or B activity as measured by hemagglutination inhibition. It is suggested that the method of purification influences the carbohydrate and amino acid yields.
D000116:Acetylgalactosamine / Q000032:analysis*; D000596:Amino Acids / Q000032:analysis; D000937:Antigen-Antibody Reactions; D002241:Carbohydrates / Q000032:analysis; D002272:Carcinoembryonic Antigen / Q000302:isolation & purification*; D006528:Carcinoma, Hepatocellular / Q000032:analysis; D002847:Chromatography, Agarose; D003110:Colonic Neoplasms / Q000032:analysis; D005688:Galactosamine / Q000031:analogs & derivatives*; D005690:Galactose / Q000032:analysis; D006595:Hexosamines / Q000032:analysis; D006801:Humans; D005779:Immunodiffusion; D008113:Liver Neoplasms; D009362:Neoplasm Metastasis; D012794:Sialic Acids / Q000032:analysis
D000596:Amino Acids; D002241:Carbohydrates; D002272:Carcinoembryonic Antigen; D006595:Hexosamines; D012794:Sialic Acids; D005688:Galactosamine; D000116:Acetylgalactosamine; D005690:Galactose