Effects of hypothalamic extract and dibutyryl cyclic adenosine monophosphate on follicle-stimulating hormone production in the rat adenohypophysis in vitro.
An assay system, using the characteristic migration of follicle-stimulating hormone (FSH) on polyacrylamide gels, was developed to evaluate the effects of hypothalamic factors on amino acid incorporation into FSH in the rat adenohypophysis incubated in vitro. Follicle-stimulating hormone extracted from rat pituitary secretory granules was found to migrate on 7-5% polyacrylamide gels, pH 9-5, as a protein with an Rf of 0-614. The characteristic Rf was used to measure [14C]amino acid incorpotation into FSH in rat adenohypophyses in vitro. Follicle-stimulating hormone synthesis increased after addition of crude hypothalamic extract to the incubation medium. Hormone synthesis was also increased three times after the addition of dibutyryl cyclic AMP.
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001681:Biological Assay; D003994:Bucladesine / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D005640:Follicle Stimulating Hormone / Q000096:biosynthesis* / Q000302:isolation & purification*; D007031:Hypothalamus / Q000502:physiology*; D066298:In Vitro Techniques; D008297:Male; D010902:Pituitary Gland / Q000378:metabolism*; D051381:Rats; D013268:Stimulation, Chemical
D000596:Amino Acids; D003994:Bucladesine; D005640:Follicle Stimulating Hormone