Interactions of lectins with plasma membrane glycoproteins of the Ehrlich ascites carcinoma cell.
Several aspects of the interaction of various lectins with the surface of Ehrlich ascites carcinoma cells are described. The order of agglutinating activity for various lectins is Ricinus communis greater than wheat germ greater than or equal to concanavalin A greater than or equal to soybean greater than Limulus polyphemus. No agglutination was noted for Ulex europaeus. Using 125I-labeled lectins it was determined that there are 1.6 and 7 times as many Ricinus communis lectin binding sites for concanavalin A and soybean lectins. Sodium deoxycholate-solubilized plasma membrane material was subjected to lectin affinity chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The lectin receptors of the plasma membrane appeared to be heterogeneous and some qualitative differences could be discerned among the electrophoretically analyzed material, which bound to and was specifically eluted from the various lectin affinity columns. The characteristics of elution of bound material from individual lectin columns indicated secondary hydrophobic interactions between concanavalin A or wheat germ agglutinin and their respective lectin receptor molecules.
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000372:Agglutination Tests; D000818:Animals; D001665:Binding Sites; D002286:Carcinoma, Ehrlich Tumor / Q000032:analysis / Q000378:metabolism*; D002462:Cell Membrane / Q000032:analysis / Q000378:metabolism; D002784:Cholesterol / Q000032:analysis; D002846:Chromatography, Affinity; D003840:Deoxycholic Acid; D008058:Dihydrolipoamide Dehydrogenase / Q000378:metabolism; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism*; D037102:Lectins / Q000378:metabolism*; D051379:Mice; D009363:Neoplasm Proteins / Q000378:metabolism*; D010743:Phospholipids / Q000032:analysis; D011485:Protein Binding; D012794:Sialic Acids / Q000032:analysis; D013347:Subcellular Fractions / Q000378:metabolism; D013385:Succinate Dehydrogenase / Q000378:metabolism
D006023:Glycoproteins; D037102:Lectins; D009363:Neoplasm Proteins; D010743:Phospholipids; D012794:Sialic Acids; D003840:Deoxycholic Acid; D002784:Cholesterol; D013385:Succinate Dehydrogenase; D008058:Dihydrolipoamide Dehydrogenase; D005952:Glucose-6-Phosphatase; D000251:Adenosine Triphosphatases