Production of B-tropic murine leukemia virus by somatic cell hybrids between mouse peritoneal macrophages and simian virus 40-transformed human cells.
Simian virus 40 (SV40)-transformed human cells (LN-SV) were fused with BALB/c peritoneal macrophages (BALB/c X LN-SV) and with C57BL peritoneal macrophages (C57BL X LN-SV) and hybrid clones, all of which had segregated human chromosomes and contained the entire complement of mouse chromosomes, were isolated. All 15 BALB/c X LN-SV hybrid clones were producing varying titers (10 to 10(6) plaque-forming units/ml) of B-tropic murine leukemia virus, whereas none of the nine C57BL X LN-SV hybrid clones was producing detectable ecotropic murine leukemia virus.
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D006801:Humans; D006822:Hybrid Cells / Q000382:microbiology*; D009052:Leukemia Virus, Murine / Q000254:growth & development*; D008264:Macrophages; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D013539:Simian virus 40; D013045:Species Specificity; D014779:Virus Replication