title: Characteristics of mammary tumor cultures from four mouse strains infected with mammary tumor virus.
abstract: The morphology and production of oncornaviruses in primary cell and explant mammary adenocarcinoma cultures derived from C3Hf, BALB/cNIV, BALB/cfC3H, and C3H mice were characterized. Cultures from the four mouse strains were morphologically similar; they were all epithelial and formed hemicysts and mounds. However, the numbers of cells containing mammary tumor virus (MTV) antigens and the production of cell-free MTV varied greatly among the established cultures. The percentages of MTV-positive cells in the cultures correlated with detection of cell-free MRV; immunodiffusion assays of virions from C3Hf and BALB/cNIV cultures were consistently negative for MTV antigens, whereas virions from BALB/cfC3H and C3H cultures were always positive. Synthesis of murine leukemia virus group-specific, soluble antigens was always observed in C3Hf, BALB/cNIV, and C3H cultures but only rarely observed in BALB/cfC3H cultures.
mesh_terms: D000230:Adenocarcinoma / Q000382:microbiology / Q000473:pathology; D000818:Animals; D000956:Antigens, Viral; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D005838:Genotype; D005779:Immunodiffusion; D008325:Mammary Neoplasms, Experimental / Q000382:microbiology / Q000473:pathology; D008324:Mammary Tumor Virus, Mouse / Q000276:immunology / Q000302:isolation & purification; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008815:Mice, Inbred Strains
chemical_list: D000956:Antigens, Viral