Action of ethyl and methyl methane sulfonates on DNA injection and genetic recombination in T7 bacteriophage.
After treatment with methyl or ethyl methane sulfonate, T7 amber mutants display a reduced capacity for recombination. Moreover, alkylation reduces recombination frequency involving markers on the right-hand side of the genetic map more than it reduces recombination frequency involving markers on the left-hand side. We interpret this to mean that alkylation can stop DNA injection at any point along the DNA molecule, and that T7 phage injects its DNA in a unique fashion starting from the end carrying the genes for early proteins.
D000478:Alkylation; D003090:Coliphages / Q000378:metabolism*; D004279:DNA, Viral / Q000096:biosynthesis / Q000378:metabolism*; D005020:Ethyl Methanesulfonate / Q000494:pharmacology*; D008698:Mesylates / Q000494:pharmacology*; D008741:Methyl Methanesulfonate / Q000494:pharmacology*; D009154:Mutation; D011995:Recombination, Genetic / Q000187:drug effects*
D004279:DNA, Viral; D008698:Mesylates; D005020:Ethyl Methanesulfonate; D008741:Methyl Methanesulfonate