Complementation analysis of eleven tryptophanase mutations in Escherichia coli.
Nine independent mutants deficient in tryptophanase activity were isolated. Each mutation was transferred to a specialized transducing phage that carries the tryptophanase region of the Escherichia coli chromosome. The nine phages thus produced, and a tenth carrying a previously characterized tryptophanase mutation, were used to lysogenize a bacterial strain harbouring a mutation in the tryptophanase structural gene and also a suppressor of polarity. In no case was complementation observed; we conclude that there is no closely linked positive regulatory gene for tryptophanase.
D010582:Bacteriophage lambda / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005809:Genes, Regulator; D005816:Genetic Complementation Test; D008190:Lyases / Q000235:genetics*; D009154:Mutation; D014161:Transduction, Genetic; D014368:Tryptophanase / Q000235:genetics* / Q000378:metabolism
D008190:Lyases; D014368:Tryptophanase