Requirement for cellular protein synthesis in reversal of ethidium-bormide-induced inhibition of cell transformation by murine sarcoma virus.
Cultures of mouse Balb 3T3 fibroblasts exposed to a noncytotoxic dose of ethidium bromide for 16-18 hr are unable to produce foci after infection with murine sarcoma virus. Such cultures regain susceptibility to infection when incubated for 6-8 hr in drug-free growth medium. Pretreated but not untreated cultures exhibit sensitivity toward brief (6 hr) exposure to cycloheximide, chloramphenicol, and actinomycin D before infection. Pretreatment with cordy-cepin inhibits the ability of cultures to produce foci after infection. The recovery of ethidium-bromide-treated cultures requires the synthesis of cellular proteins which may have some important role in the establishment of RNA tumor virus infection.
D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D002701:Chloramphenicol / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D003839:Deoxyadenosines / Q000494:pharmacology; D004996:Ethidium / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects; D017988:Gammaretrovirus / Q000254:growth & development*; D014176:Protein Biosynthesis; D009053:Sarcoma Viruses, Murine / Q000254:growth & development*; D013997:Time Factors
D003839:Deoxyadenosines; D003609:Dactinomycin; D002701:Chloramphenicol; D003513:Cycloheximide; D004996:Ethidium