Intracellular enzyme liberation in primate spinal cord injury.
Intracellular lysosomal and nonlysosomal enzymes, as well as tissue edema, were measured in spinal cords of monkeys up to 20 days following a 300 gm-cm open injury. Although edema was maximal between six hours and 11 days, enzyme elevation was delayed. Lysosomal enzyme acid cathepsin increased beginning at five days and the beta-glucuronidase and beta-glycerophosphatase increase began at 11 days. Nonlysosomal enzymes were either not elevated or showed a slight rise. These data suggest that edema, one of the secondary damaging factors in spinal injury, is not a result of release of these intracellular enzymes. Also it appears that intracellular enzymes do not participate in early secondary damaging processes in severe spinal injury.
D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002403:Cathepsins / Q000378:metabolism*; D004487:Edema / Q000201:enzymology; D005966:Glucuronidase / Q000378:metabolism*; D005994:Glycerophosphates; D000882:Haplorhini; D007424:Intracellular Fluid / Q000201:enzymology; D008247:Lysosomes / Q000201:enzymology; D008253:Macaca mulatta; D010447:Peptide Hydrolases / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D013116:Spinal Cord / Q000201:enzymology; D013119:Spinal Cord Injuries / Q000201:enzymology*; D013997:Time Factors
D005994:Glycerophosphates; D000469:Alkaline Phosphatase; C020674:glycerol-2-phosphatase; D000135:Acid Phosphatase; D010744:Phosphoric Monoester Hydrolases; D005966:Glucuronidase; D002403:Cathepsins; D010447:Peptide Hydrolases