Effect of diethylaminoethyl-dextran on the replication of a murine sarcoma (Moloney)-leukemia virus complex in mouse embryo cultures (38577).
Isolates of a sarcoma (Moloney)-leukemia virus complex prepared as cell-free extracts from mouse tumors showed no enhancement in infectivity by DEAE-D either in the sarcoma moiety measured by focus formation or in the leukemia moiety measured by XC cell assay.  The sarcoma moiety was not enhanced by DEAE-D in MEM and modified McCoy's 5a media or when varying amounts of FCS and glutamine were included in the media.  Progressive enhancement of viral infectivity by DEAE-D was found when the viral preparations were passaged serially in MEF cells.  DEAE-D ALSO ENHANCED TUMOR FORMATION IN VIVO BY TISSUE CULTURE PASSAGED VIRUS.
D000818:Animals; D002474:Cell-Free System; D002478:Cells, Cultured; D003470:Culture Media; D003588:Cytopathogenic Effect, Viral; D003911:Dextrans / Q000031:analogs & derivatives* / Q000494:pharmacology; D004048:Diethylamines / Q000494:pharmacology; D004622:Embryo, Mammalian; D017988:Gammaretrovirus / Q000187:drug effects* / Q000472:pathogenicity; D005973:Glutamine / Q000494:pharmacology; D051379:Mice; D008979:Moloney murine leukemia virus / Q000187:drug effects* / Q000472:pathogenicity; D012509:Sarcoma / Q000382:microbiology; D010948:Viral Plaque Assay; D014774:Virulence; D014779:Virus Replication / Q000187:drug effects*
D003470:Culture Media; D003911:Dextrans; D004048:Diethylamines; D005973:Glutamine