Stereospecificity of hydrogen transfer by phosphoglycerate dehydrogenase.
Phosphoglycerate dehydrogenase (EC 1.1.1.95) has been shown to be A site specific in its hydrogen transfer capacity unlike other dehydrogenases which use phosphorylated substrates. The experiments have been carried out using a coupled assay system with yeast alcohol dehydrogenase. The specific activity measurements of the reaction products indicate the possible influence of an isotope effect on this system.
D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D002237:Carbohydrate Dehydrogenases / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D006859:Hydrogen / Q000378:metabolism; D007700:Kinetics; D009243:NAD; D010084:Oxidation-Reduction; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D014316:Tritium
D009243:NAD; D014316:Tritium; D006859:Hydrogen; D000429:Alcohol Oxidoreductases; D002237:Carbohydrate Dehydrogenases; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases