A 3'-deoxymononucleotide-producing nuclease from the marine sponge Verongia aerophoba. I. Purification.
A four-step procedure for purification of a nuclease from the keratinous sponge Verongia aerophoba is described. The extracted material is lyophilized, acidified, and subjected to chromatography on Sephadex, hydroxyapatite, and phosphocellulose. The nuclease is purified about 1,000-fold from the crude extract and approximately 1,600-fold from concomitant acid RNase. Phosphodiesterase is lost after chromatography on Sephadex. The purified enzyme solution contains one single activity as determined by polyacrylamide gel electrophoresis.
D000135:Acid Phosphatase / Q000032:analysis; D000818:Animals; D002845:Chromatography; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D003851:Deoxyribonucleases / Q000302:isolation & purification* / Q000378:metabolism; D004589:Electrophoresis, Disc; D005092:Exonucleases / Q000302:isolation & purification* / Q000378:metabolism; D005612:Freeze Drying; D006882:Hydroxyapatites; D010727:Phosphoric Diester Hydrolases / Q000032:analysis; D011161:Porifera / Q000201:enzymology*; D012260:Ribonucleases / Q000032:analysis
D006882:Hydroxyapatites; D003851:Deoxyribonucleases; D005092:Exonucleases; D012260:Ribonucleases; D000135:Acid Phosphatase; D010727:Phosphoric Diester Hydrolases