title: Ultrastructural changes occurring during germination and outgrowth of spores of the thermophile Bacillus acidocaldarius.
abstract: Spores of the thermophilic, acidophilic, Bacillus acidocaldarius were covered by a thick outer coat and a laminated inner coat (5.5 nm periodicity). Small membranous vesicles were present in the spore core and they disappeared as germination proceeded. After depolymerization of the cortex, and a 30% increase in spore diameter a localized gap appeared in the laminated inner coat only. This inner coat gap was narrow and could be the whole length of the spore. The germ cell appeared to grow, or to be pushed towards the inner coat gap, at which stage the outer coat disappeared in the same localized area. As the vegetative cell grew out the spore coat fell away, with loose cortical material still attached to it. The young germ cell developed a large spherical electron dense inclusion body in the cytoplasm, at the same time as the ribosomal and nuclear areas became distinct.
mesh_terms: D001407:Bacillus / Q000254:growth & development / Q000648:ultrastructure*; D002467:Cell Nucleus; D003470:Culture Media; D002479:Inclusion Bodies; D008854:Microscopy, Electron; D012270:Ribosomes; D013045:Species Specificity; D013171:Spores, Bacterial / Q000254:growth & development* / Q000648:ultrastructure
chemical_list: D003470:Culture Media