title: Loss of ability to synthesize collagen in fibroblasts transformed by rous sarcoma virus.
abstract: Transformation of chick embryo fibroblasts by Rous sarcoma virus inhibited their ability to synthesize collagen. Kinetic experiments showed that 72 hours after infection, collagen synthesis was reduced by 90%. Nontransforming Rous-associated viruses did not inhibit collagen synthesis. The inhibition resulted from the failure of the cells to synthesize collagen polypeptides rather than from a decrease in the activity of prolyl hydroxylase; the levels of prolyl hydroxylase were fourfold those in uninfected cells. The addition of dibutyryl cyclic AMP and theophylline, alone or together, did not restore collagen synthesis.
mesh_terms: D000818:Animals; D001358:Avian Sarcoma Viruses; D003994:Bucladesine / Q000494:pharmacology; D002471:Cell Transformation, Neoplastic; D002642:Chick Embryo; D003094:Collagen / Q000096:biosynthesis*; D005347:Fibroblasts / Q000378:metabolism; D010452:Peptide Biosynthesis; D011393:Procollagen-Proline Dioxygenase / Q000378:metabolism; D013806:Theophylline / Q000494:pharmacology
chemical_list: D003994:Bucladesine; D003094:Collagen; D013806:Theophylline; D011393:Procollagen-Proline Dioxygenase