In vitro RNA synthesis should be coupled to pyrophosphate hydrolysis.
D003090:Coliphages / Q000378:metabolism; D004267:DNA Viruses / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism; D011756:Diphosphates / Q000378:metabolism*; D006868:Hydrolysis; D007700:Kinetics; D008433:Mathematics; D008970:Molecular Weight; D012313:RNA / Q000096:biosynthesis*; D013698:Templates, Genetic; D013816:Thermodynamics; D014158:Transcription, Genetic
D004279:DNA, Viral; D011756:Diphosphates; D012313:RNA