End-point parameter adjustment on a small desk-top programmable calculator for logit-log analysis of radioimmunoassay data.
We describe an improved method of logit-log curve fitting, by adjusting end-point parameters in radioimmunoassay studies, for use with a small desk-top programmable calculator. Straight logit-log analyses are often deficient because of their high sensitivity to small errors in the end-point parametes B0 and NSB (the actual measured activity in the tubes). The literature suggests techniques for adjusting these end-point parameters, but they require too much computing time and programming space to be used with a desk-top programmable calculator. The extension to the logit-log model presented here is easily handled by the programmable calculator and provides a good estimate of the change required in B0 and NSB to obtain a better fit. The program requires 1.5 min to run on our desk-top programmable calculator, and has resulted in improved data analysis for all of the 11 types of radioimmunoassay studied.
D000324:Adrenocorticotropic Hormone / Q000032:analysis; D000450:Aldosterone / Q000032:analysis; D003201:Computers; D013006:Growth Hormone / Q000032:analysis; D006801:Humans; D008433:Mathematics; D011863:Radioimmunoassay / Q000379:methods*; D013223:Statistics as Topic; D013739:Testosterone / Q000032:analysis; D013972:Thyrotropin / Q000032:analysis
D013739:Testosterone; D000450:Aldosterone; D000324:Adrenocorticotropic Hormone; D013972:Thyrotropin; D013006:Growth Hormone