Nongenetic complementation of group V temperature-sensitive mutants of vesicular stomatitis virus by UV-irradiated virus.
Cells infected with temperature-sensitive (ts) mutants of complementation group V of vesicular stomatitis virus (VSV) give an enhanced yield at nonpermissive temperature when co-infected or superinfected with UV-irradiated virus. Virions produced in these mixed infections are temperature sensitive and do not complement ts V45. Rescue of group V mutants is multiplicity dependent. It can occur in the presence of cycloheximide; kinetics of rescue are similar in the absence or in the presence of the drug. Rescue is due to nongenetic complementation and is interpreted as a trigger effect on maturation of a small quantity of biologically active protein V molecules provided by UV-irradiated virus. These results are comfirmed by rescue of ts V45 by UV-irradiated, defective, interferring T particles.
D003513:Cycloheximide / Q000494:pharmacology; D004307:Dose-Response Relationship, Radiation; D005347:Fibroblasts; D009154:Mutation; D011691:Puromycin / Q000494:pharmacology; D011830:Radiation Effects; D013696:Temperature; D013997:Time Factors; D014466:Ultraviolet Rays; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects / Q000254:growth & development* / Q000528:radiation effects; D014779:Virus Replication
D011691:Puromycin; D003513:Cycloheximide