[Effect on drugs changing the intracellular level of adenosine 3',5'-cyclic monophosphate on interferon formation in chick embryo cells of different ages].
The effect of theophylline and adrenaline on the synthesis of interferon induced by the influenza B virus, strain Lee, in a chick embryo tissue culture was studied. Both preparation were found to decrease interferon synthesis when 5-day-old cultures were used; the inhibitory effect was increased when the two drugs were used together. The degree of inhibition of interferon production depended on a dose of the preparation; the inhibition was still present even when the drugs ere introduced several hours after the cells were infected with interferonogen. The treatment of one-day-old cultures with theophylline resulted in increase of interferon synthesis, whereas administration of adrenaline alone or together with theophylline did not affect the level of interferon synthesis. The drugs used produced no effect on the reproduction of the test-virus of vesicular stomatitis, Newcastle disease and Chickungunya viruses in chick embryo cells and influenza B virus in the developing chick embryos. The results obtained are discussed from the point of view of a possible influence of the intracellular adenosine 3',5-cyclic monophosphate level on the synthesis of virus-induced interferon.
D000375:Aging; D000818:Animals; D002478:Cells, Cultured / Q000187:drug effects; D002642:Chick Embryo; D000242:Cyclic AMP / Q000502:physiology*; D003864:Depression, Chemical; D004305:Dose-Response Relationship, Drug; D004837:Epinephrine / Q000494:pharmacology*; D066298:In Vitro Techniques; D007372:Interferons / Q000096:biosynthesis*; D009975:Orthomyxoviridae / Q000276:immunology; D013806:Theophylline / Q000494:pharmacology*
D007372:Interferons; D013806:Theophylline; D000242:Cyclic AMP; D004837:Epinephrine
Vliianie veshchestv, izmeniaiushchikh vnutrikletochnyĭ uroven' adenozin 3', 5'-tsiklicheskogo monofosfata, na obrazovanie interferona v kletkakh kurinogo émbriona raznogo vozrasta