Acetylcholine-induced initiation of thymic lymphoblast DNA synthesis and proliferation.
Low (5 times 10-9 M to 10-7 M) acetylcholine concentrations cause a calcium-independent stimulation of the initiation of DNA synthesis and proliferation of lymphoblasts which are part of rat thymocyte populations suspended in vitro. A much higher (5 times 10-5 M) acetylcholine concentration also stimulates lymphoblast DNA synthesis and proliferation, but this action is calcium-dependent. This proliferogenic response to acetylcholine is however not clearly mediated by either cyclic GMP or cyclic AMP.
D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000096:biosynthesis; D006152:Cyclic GMP / Q000096:biosynthesis; D004247:DNA / Q000096:biosynthesis*; D008297:Male; D011863:Radioimmunoassay; D051381:Rats; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D013806:Theophylline / Q000494:pharmacology
D004247:DNA; D013806:Theophylline; D000242:Cyclic AMP; D006152:Cyclic GMP; D000109:Acetylcholine; D002118:Calcium