Determination of the association constants and maximum binding capacities between estradiol and cytosol of the uterus by the least-squares analysis of the Scatchard plot.
The association constants and the binding capacities of association of small molecules with macromolecules have been determined by the tangent analysis, the graphical analysis, and the computer data analysis, by trial and convergence of the Scatchard plot. The analytical method for the calculation of the binding parameters based on the Scatchard plot was derived and the optimum values of the binding parameters were obtained by the least squares calculation based on the analytical method. The errors by the analytical method were smaller than those by the graphical method in the equilibrium system between 3H-estradiol and some cytosols of uterus.
D003600:Cytosol; D004958:Estradiol; D005260:Female; D006801:Humans; D011956:Receptors, Cell Surface; D013223:Statistics as Topic; D014599:Uterus
D011956:Receptors, Cell Surface; D004958:Estradiol