Colchicine resistance in mammalian cell lines.
Colchicine-resistant variants derived from mouse and Syrian hamster lines are described. The resistant cells do not appear to be true mutants, since they appear at a high frequency, unaffected by treatment with ethyl methyl sulphonate, and are unstable in the absence of the drug. They are cross-resistant to other drugs, show a reduced rate of binding of colchicine in monolayer, and give extracts with colchicine-binding properties identical to those of the wild type. Thus the resistance is due to a permeability barrier. The naturally occurring resistance of the Syrian hamster line is specific for colchicine, and may be due to a specific permeability barrier. The Syrian hamster line is also shown to have an extra colchicine-binding pool.
D000818:Animals; D001665:Binding Sites; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line / Q000187:drug effects*; D003078:Colchicine / Q000378:metabolism / Q000494:pharmacology*; D006224:Cricetinae; D004351:Drug Resistance; D007700:Kinetics; D008698:Mesylates / Q000494:pharmacology; D051379:Mice; D009154:Mutation; D010539:Permeability; D011955:Receptors, Drug; D013997:Time Factors
D008698:Mesylates; D011955:Receptors, Drug; D003078:Colchicine