DNA polymerase activity of cultured rheumatoid synovial cells.
Cultured synovial cells from 8 patients with rheumatoid arthritis (RA) and 7 subjects without joint disease were assayed and comapred for RNA-directed and DNA-directed DNA polymerase activity. No activity was found in either RA or control specimens using a synthetic RNA template that specificically detects oncornavirus RNA-directed DNA polymerase. The DNA-directed DNA polymerase activity of RA specimens was increased (P less than 0.10) in the high-speed pellet fraction of cell lysates. The possible relationship of these finding to virus infection in RA is disscussed.
D000328:Adult; D000368:Aged; D001172:Arthritis, Rheumatoid / Q000201:enzymology*; D002458:Cell Fractionation; D046508:Culture Techniques; D004254:DNA Nucleotidyltransferases / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009053:Sarcoma Viruses, Murine / Q000201:enzymology; D013583:Synovial Membrane / Q000166:cytology / Q000201:enzymology*; D013698:Templates, Genetic
D004254:DNA Nucleotidyltransferases