Ethidium bromide inhibits appearance of closed circular viral DNA and integration of virus-specific DNA in duck cells infected by avian sarcoma virus.
The DNA of avian sarcoma virus assumes a closed circular configuration before integration into the host cell chromosomal DNA. Ethidium bromide reduces the formation of superhelical viral DNA and concurrently blocks integration of the viral genome. Inhibition of integration of viral DNA results in the inhibition of virus replication.
D001355:Alpharetrovirus / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D004270:DNA, Circular / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism*; D003851:Deoxyribonucleases / Q000378:metabolism; D004372:Ducks; D004996:Ethidium / Q000494:pharmacology*; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000096:biosynthesis; D014316:Tritium; D014529:Uridine / Q000378:metabolism; D014779:Virus Replication / Q000187:drug effects
D004270:DNA, Circular; D004279:DNA, Viral; D012367:RNA, Viral; D014316:Tritium; D003851:Deoxyribonucleases; D004996:Ethidium; D014529:Uridine