title: Electron spin resonance for detecting polyadenylate tracts in RNA's.
abstract: Electron spin resonance is used to detect RNA's that contain polyadenylate tracts. The method depends on the ability of RNA's that contain polyadenylate sequences to associate with poly(2'-deoxy-2'-fluoro)uridylic acid, which has been spin-labeled with 4-(2-iodoacetamido)-2,2,6,6-tetramethylpiperidinooxyl. The formation of the hybridization product can be detected by monitoring the decrease in mobility of the spin probe.
mesh_terms: D000227:Adenine Nucleotides / Q000032:analysis*; D000818:Animals; D004578:Electron Spin Resonance Spectroscopy; D009691:Nucleic Acid Denaturation; D011119:Polynucleotides / Q000032:analysis*; D012313:RNA / Q000032:analysis*; D012333:RNA, Messenger / Q000032:analysis; D011817:Rabbits; D013113:Spin Labels; D014500:Uracil Nucleotides / Q000032:analysis
chemical_list: D000227:Adenine Nucleotides; D011119:Polynucleotides; D012333:RNA, Messenger; D013113:Spin Labels; D014500:Uracil Nucleotides; D012313:RNA