Effects of concanavalin A and a succinylated derivative on lymphocyte proliferation and cyclic nucleotide levels.
To better define cell surface-related changes involved in lymphocyte activation, we studied native concanavalin A (Con A) and succinylated concanavalin A (Suc-Con A) for their effects on proliferation and cyclic nucleotide levels of human peripheral blood lymphocytes. At optimal mitogenic concentrations, the two forms of Con A induce equivalent proliferation; however, the mitogenic activity of Con A progressively decreases above 50 mug/ml. In contrast, the mitogenic activity of Suc-Con A is not decreased even at 250 mug/ml.
D002455:Cell Division; D002462:Cell Membrane / Q000648:ultrastructure; D003208:Concanavalin A / Q000031:analogs & derivatives* / Q000494:pharmacology*; D000242:Cyclic AMP / Q000378:metabolism*; D006152:Cyclic GMP / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007700:Kinetics; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000378:metabolism / Q000502:physiology / Q000648:ultrastructure; D013329:Structure-Activity Relationship
D003208:Concanavalin A; D000242:Cyclic AMP; D006152:Cyclic GMP