Temperature dependence of strand separation of the DNA molecules containing integrated SV40 DNA in transformed cells.
It has previously been demonstrated that the DNA molecules containing a genetic marker from one region of a bacterial genome undergo complete strand separation at temperatures usually different from the molecules containing a genetic marker from another region of the genome. The experiments also showed that if a group of molecules undergo complete strand separation over a narrow temperature range, of the order of one to three degrees, it is highly likely that they all come from one region of the bacterial genome. The purpose of the work reported here was to establish appropriate procedures for doing a similar analysis of the DNA molecules containing the integrated SV40 DNA in transformed mouse cells. One result of interest is that in the 11A8 cell line about 40 per cent of the integrated SV40 DNA detectable by an RNA-DNA hybridization assay can be accounted for by a group of molecules which undergo complete strand separation within a 1.0 degree C interval.
D001665:Binding Sites; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D004262:DNA Restriction Enzymes; D004270:DNA, Circular; D004273:DNA, Neoplasm; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D003851:Deoxyribonucleases; D004720:Endonucleases; D004926:Escherichia coli / Q000201:enzymology; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000096:biosynthesis; D013539:Simian virus 40 / Q000378:metabolism*; D013696:Temperature
D004270:DNA, Circular; D004273:DNA, Neoplasm; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D012367:RNA, Viral; D012321:DNA-Directed RNA Polymerases; D003851:Deoxyribonucleases; D004720:Endonucleases; D004262:DNA Restriction Enzymes