Specific oligoribonucleotide synthesis using primer-independent polynucleotide phosphorylase.
Oligoribonucleotides of a predetermined base sequence beginning with adenylyl-3', 5'-adenosine at the 5' end have been synthesized in yields varying between 13% and 42%. The synthesis was carried out using primer-independent polynucleotide phosphorylase from E. coli in the presence of high concentrations of primer and of sodium chloride.
D001483:Base Sequence; D004926:Escherichia coli / Q000201:enzymology; D007700:Kinetics; D009841:Oligonucleotides / Q000096:biosynthesis*; D009843:Oligoribonucleotides / Q000096:biosynthesis*; D010727:Phosphoric Diester Hydrolases; D011117:Polyribonucleotide Nucleotidyltransferase / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology
D009841:Oligonucleotides; D009843:Oligoribonucleotides; D012965:Sodium Chloride; D011117:Polyribonucleotide Nucleotidyltransferase; D010727:Phosphoric Diester Hydrolases