Two mechanisms of near-ultraviolet lethality in Saccharomyces cerevisiae: a respiratory capacity-dependent and an irreversible inactivation.
Near-ultraviolet irradiation of actively growing yeast cells leads to cell death by two distinct mechanisms. The first type of cell death is evident after low doses of near-ultraviolet light (3 times 10-4 ergs times mm- minus 2) and is due to a reversible inactivation of the respiratory capacity of the cell. In studies with yeast mitochondrial membranes the quinones were identified as the site of inactivation by determining the relative levels of the following oxidase activities after irradiation: exogenous NADH, endogenous NADH (via isocitrate dehydrogenase), succinate, and D-lactate oxidases. A second type of cell death is caused after high doses (1.8 times 10-5 ergs times mm- minus 2) and is irreversible. The mechanism of this inactivation is unknown.
D004579:Electron Transport / Q000528:radiation effects*; D007770:L-Lactate Dehydrogenase / Q000528:radiation effects; D008566:Membranes / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D010101:Oxygen Consumption / Q000528:radiation effects*; D011830:Radiation Effects; D012441:Saccharomyces cerevisiae / Q000528:radiation effects*; D013385:Succinate Dehydrogenase / Q000528:radiation effects; D014466:Ultraviolet Rays
D009243:NAD; D007770:L-Lactate Dehydrogenase; D013385:Succinate Dehydrogenase