In-vitro cultivation of human cytomegalovirus in thyroid epithelial cells.
Several strains of human cytomegalovirus including recent isolates, were grown in epithelial cells derived from thyroid tissue. All the strains tested grew in these cultures without pre-treatment of the cells, and no difference in cytopathic effect was detected between strains of genital and non-genital origin. 4 strains of CMV were isolated directly from urine in thyroid cells; however the failure to isolate 6 further strains in these cultures from other specimens may indicate that a higher multiplicity of infection is required to infect epithelial than fibroblast cells.
D002584:Cervix Uteri / Q000382:microbiology; D046508:Culture Techniques; D003587:Cytomegalovirus / Q000254:growth & development* / Q000302:isolation & purification; D003588:Cytopathogenic Effect, Viral; D005260:Female; D006801:Humans; D013961:Thyroid Gland / Q000382:microbiology*; D014556:Urine / Q000382:microbiology; D014776:Virus Cultivation / Q000379:methods*; D014779:Virus Replication