[The fructose induced "glycogenosis". II. Histochemical studies of glycogen metabolism in rat liver after fructose overload and similar diets (author's transl)].
BACKGROUND
Feeding of fructose for 7 days has been morphometrically shown to induce a SER-reduction and an accumulation of glycogen in rat liver cells. This hypothetical model "glycogenosis" is investigated with histochemical methods.


METHODS
Rats are given a solution of 60% fructose in water as only nutritional source. Controls are given a solution of 60% glucose in water, an isocaloric Altromin-R-standard diet and an Altromin-R-standard diet ad libitum. Reversion of fructose induced metabolic changes is investigated by a 7 days fructose diet followed by an 1-4 days Altromin-R-standard diet ad libitum. Glycogen and glycogen metabolizing enzymes are demonstrated after a 7 days diet and in the course of an 1-7 days fructose diet.


CONCLUSIONS
Feeding of fructose leads to a high glycogen content, combined with a high activity of glycogen-phosphorylase and glucose-6-phosphatase in the liver parenchyma. Glycogen-synthetase activity increases during the first 4 days and then it drops to a low level. A pathological alteration of liver cell metabolism seems to be improbable, for all fructose induced changes are reversibel after 2 days of Altromin-R-standard diet. Glucose-6-phosphatase, as a marker-enzyme of the smooth endoplasmatic reticulum, is discussed to become activated by disruption of SER membranes due to fructose.
D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D004195:Disease Models, Animal; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D005632:Fructose / Q000633:toxicity*; D005947:Glucose / Q000494:pharmacology; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D006008:Glycogen Storage Disease / Q000139:chemically induced*; D005953:Glycogen Storage Disease Type I / Q000139:chemically induced; D006006:Glycogen Synthase / Q000378:metabolism; D006651:Histocytochemistry; D008099:Liver / Q000201:enzymology / Q000648:ultrastructure; D008112:Liver Glycogen / Q000378:metabolism; D008297:Male; D006005:Phosphorylases / Q000378:metabolism; D051381:Rats
D008112:Liver Glycogen; D005632:Fructose; D006005:Phosphorylases; D006006:Glycogen Synthase; D000135:Acid Phosphatase; D005952:Glucose-6-Phosphatase; D005947:Glucose
Die Fruktose-induzierte "Glykogenose". II. Histochemische Untersuchungen zum Glykogenstoffwechsel der Rattenleber nach Fruktosebelastung und bei vergleichbaren Diäten