Specific adenosine binding proteins from rat liver.
Specific adenosine-binding proteins from homogenates of rat liver have been fractionated on a DEAE-cellulose column. Three major peaks have been identified with respect to histone phosphokinase and cAMP and adenosine-binding activities. Peak I contains only histone phosphokinase activity not stimulated by cAMP. Peak II contains histone phosphokinase slightly stimulated by cAMP. Both cAMP- and adenosine-binding activities are found in this fraction. The major adenosine-binding protein is associated with Peak III. Histone phosphokinase in Peak III which also binds cAMP is stimulated 2-fold by 2.5 muM cAMP whereas adenosine at 2.5 X 10(-4)M inhibits these enzymes equally well in each of three peaks. The specificity of adenosine binding is discussed.
D000241:Adenosine / Q000031:analogs & derivatives / Q000032:analysis* / Q000378:metabolism; D000818:Animals; D001667:Binding, Competitive; D002352:Carrier Proteins / Q000032:analysis*; D002848:Chromatography, DEAE-Cellulose; D000242:Cyclic AMP / Q000032:analysis; D006801:Humans; D008099:Liver / Q000032:analysis* / Q000378:metabolism; D006656:Protamine Kinase / Q000032:analysis; D051381:Rats
D002352:Carrier Proteins; D000242:Cyclic AMP; D006656:Protamine Kinase; D000241:Adenosine