Production of yeast alcohol dehydrogenase isoenzymes by selection.
Mutants of yeast alcohol dehydrogenase have been produced that protect the cell against the poisonous aldehyde acrolein by increasing the NADH-NAD ratio. The altered properties include changes both in binding constants and in cooperativity. Such mutants may be useful in exploring the nature of adaptation at the molecular level.
D000429:Alcohol Oxidoreductases; D005075:Biological Evolution; D005796:Genes; D007527:Isoenzymes; D007700:Kinetics; D009154:Mutation; D009243:NAD / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology
D007527:Isoenzymes; D009243:NAD; D000429:Alcohol Oxidoreductases