The carboxylic acid groups of bovine luteinizing hormone. The effects of their modification on receptor site binding and subunit-subunit interaction.
The modification of the carboxyl groups of the subunits of bovine luteinizing hormone to neutral derivatives by carbodiimide-mediated coupling with glycine methyl ester has been studied. The modified alpha subunit, which has 8 residues of glycine methyl ester incorporated, will no longer recombine with native beta (hormone-specific) subunit, but the modified beta subunit, with 6 to 7 glycine methyl esters incorporated, will recombine with native alpha to yield a partially active hormone. Derivatization of the intact hormone results in dissociation to subunits together with formation of a major side product which is covalently cross-linked. Significant cross-linked product was not obtained during modification of individual subunits, thus indicating an orientation between an activated carboxyl group(s) and a nucleophile(s) in the intact hormone which favors coupling. Separation of subunits from the derivatized, noncross-linked fraction by countercurrent distribution reveals a heterogeneous preparation of the modified alpha subunit which also will not recombine with either a native or modified beta subunit. The beta subunit from the modified intact hormone was indistinguishable from the modified isolated beta subunit in amino acid composition and in ability to recombine with native alpha subunit. The results are consonant with data from this and other laboratories in which various modifications of the alpha chain, the subunit common to the glycoproteins, more seriously affect recombination than similar modifications of the beta subunits. The number of carboxyl groups modified in each subunit is compatible with but not in total agreement with assignments of amides reported from sequence studies.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000600:Amino Acids, Dicarboxylic / Q000032:analysis*; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism; D007986:Luteinizing Hormone / Q000378:metabolism; D046911:Macromolecular Substances; D008297:Male; D011485:Protein Binding; D011487:Protein Conformation; D051381:Rats; D011956:Receptors, Cell Surface; D012756:Sheep; D013045:Species Specificity; D013552:Swine; D013737:Testis / Q000378:metabolism
D000596:Amino Acids; D000600:Amino Acids, Dicarboxylic; D046911:Macromolecular Substances; D011956:Receptors, Cell Surface; D007986:Luteinizing Hormone