title: Assembly of the mitochondrial membrane system. Cytoplasmic mutants of Saccharomyces cerevisiae with lesions in enzymes of the respiratory chain and in the mitochondrial ATPase.
abstract: Mutants of Saccharomyces cervisiae with defects in enzymes of the electron transfer chain and in the rutamycin-sensitive ATPase have been isolated. Some of the mutants are specifically affected in either cytochrome oxidase, coenzyme QH2-cytochrome c reductase or ATPase. Other strains are deficient in both cytochrome oxidase and coenzyme QH2-cytochrome c reductase but still have rutamycin-sensitive ATPase. All the mutants reported in this study fail to be complemented by a rho0 tester derived from a respiratory competent strain. The meiotic spore progeny obtained by mating the mutants to a respiratory competent haploid yeast, when scored for growth on glycerol, show a non-Mendelian segregation of the phenotype. These two genetic tests indicate the mutations to be cytoplasmically inherited.
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D003579:Cytochrome Reductases / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000378:metabolism*; D005816:Genetic Complementation Test; D008566:Membranes / Q000187:drug effects / Q000201:enzymology; D008928:Mitochondria / Q000201:enzymology*; D009154:Mutation; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D010641:Phenotype; D012427:Rutamycin / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D013172:Spores, Fungal / Q000378:metabolism; D014451:Ubiquinone
chemical_list: D014451:Ubiquinone; D012427:Rutamycin; D003579:Cytochrome Reductases; D009251:NADPH-Ferrihemoprotein Reductase; D003576:Electron Transport Complex IV; D000251:Adenosine Triphosphatases