[A fluorometic test for microsomal monooxygenase activity in the rat liver with scoparone as substrate (author's transl)].
A sensitive fluorometric test for the determination of monooxygenase activity in liver microsomes from rats is described. The assay is based on the O-demethylation of 6,7-dimethoxycoumarin (scoparone) to 7-hydroxy-6-methoxycoumarin and 6-hydroxy-7-methoxycoumarin. Pretreatment of rats with phenobarbital or polycyclic hydrocarbons (benzo[a]pyrene, 3-methylcholanthrene) causes a significant increase in the amount of microsomal dealkylation activity.
D000818:Animals; D003374:Coumarins; D008862:Microsomes, Liver / Q000201:enzymology*; D010105:Oxygenases / Q000378:metabolism*; D051381:Rats; D013050:Spectrometry, Fluorescence / Q000379:methods
D003374:Coumarins; D010105:Oxygenases
Eine fluorometrische Bestimmungsmethode für mikrosomale Monooxygenase-Aktivität der Rattenleber mit Scoparon als Substrat.