Biochemical studies on sulfate-reducing bacteria. XIV. Enzyme levels of adenylylsulfate reductase, inorganic pyrophosphatase, sulfite reductase, hydrogenase, and adenosine triphosphatase in cells grown on sulfate, sulfite, and thiosulfate.
Sulfate-reducing bacteria, Desulfovibrio vulgaris, strain Miyazaki, were grown on either sulfate, sulfite, or thiosulfate as the terminal electron acceptor. Better growth was observed on sulfite and less growth on thiosulfate than on sulfate. Enzyme levels of adenylylsulfate (APS) reductase [EC 1.8.99.2], reductant-activated inorganic pyrophosphatase [EC 3.6.1.1], sulfite reductase [EC 1.8.99.1] (desulfoviridin), hydrogenase [EC 1.12.2.1], and Mg2+-activated ATPase [EC 3.6.1.3] were compared in crude extracts of these cells at various stages of growth. 1) The specific activity of APS reductase in sulfite-grown cells was only one-fourth that in sulfate-grown cells throughout growth. Thiosulfate-grown cells had an activity intermediate between those of sulfate- and sulfite-grown cells. 2) Cells grown on sulfite had lower specific activity of reductant-activated inorganic pyrophosphatase than cells grown on sulfate or thiosulfate. 3) The specific activity of sulfite reductase (desulfoviridin) was highest in sulfite-grown cells. The sulfite medium gave the enzyme in high yield as well as with high specific activity. 4) The specific activities of hydrogenase and Mg2+-ATPase were not significantly altered by electron acceptors in the growth medium.
D000249:Adenosine Monophosphate; D000251:Adenosine Triphosphatases / Q000378:metabolism; D003574:Cytochrome c Group; D003901:Desulfovibrio / Q000201:enzymology*; D008274:Magnesium / Q000494:pharmacology; D010088:Oxidoreductases / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D011755:Pyrophosphatases / Q000378:metabolism; D013431:Sulfates / Q000378:metabolism*; D013447:Sulfites / Q000378:metabolism*; D013464:Sulfuric Acids; D013885:Thiosulfates / Q000378:metabolism*
D003574:Cytochrome c Group; D011506:Proteins; D013431:Sulfates; D013447:Sulfites; D013464:Sulfuric Acids; D013885:Thiosulfates; D000249:Adenosine Monophosphate; D010088:Oxidoreductases; D000251:Adenosine Triphosphatases; D011755:Pyrophosphatases; D008274:Magnesium