title: Early events after infection of Escherichia coli by bacteriophage T5. II. Control of the bacteriophage-induced 5'-nucleotidase activity.
abstract: The control of activity of the bacteriophage T5-induced 5'-nucleotidase is dependent upon the amount of T5 parental DNA injected into the cell and expressed. When only the first-step transfer DNA is injected and expressed the amount of 5'-nucleotidase activity observed is two to three times the maximum amount observed after normal T5 infection, and inactivation of the enzyme does not occur. Enzyme inactivation occurs only after the remaining DNA is injected, but only limited expression of this DNA is required. The control of the nucleotidase inactivation process is similar to that for the repair of the nicks in parental DNA, and is probably mediated by a class IIa protein.
mesh_terms: D002118:Calcium / Q000494:pharmacology; D002474:Cell-Free System; D002701:Chloramphenicol / Q000494:pharmacology; D003087:Colicins / Q000494:pharmacology; D004279:DNA, Viral / Q000378:metabolism*; D004790:Enzyme Induction; D004926:Escherichia coli / Q000378:metabolism*; D009154:Mutation; D009708:Nucleotidases / Q000378:metabolism*; D010770:Phosphotransferases / Q000378:metabolism; D014764:Viral Proteins / Q000096:biosynthesis
chemical_list: D003087:Colicins; D004279:DNA, Viral; D014764:Viral Proteins; D002701:Chloramphenicol; D010770:Phosphotransferases; D009708:Nucleotidases; D002118:Calcium