Competitive nephelometric immunoassay method for antiepileptic drugs in patient blood.
We established a competitive nephelometric immunoassay method for the determination of the widely used antiepileptic drugs, phenobarbital and diphenylhydantoin, in human blood plasma. The anti-drug antiserum was collected from the immunized rabbit. The human serum albumin possessing numerous drug moieties was easily prepared for the assay reagent. A laser nephelometer was used to measure the scattered light from the immunoprecipitate. The assay was rapid (incubation time 10 min), required as little as 6 microliters of plasma, required neither troublesome pretreatment nor separation procedure. The patients' plasma specimens were analyzed, and the values correlated well to those determined by the high-performance liquid chromatography (correlation coefficient: 0.983 for phenobarbital, 0.987 for diphenylhydantoin).
D000818:Animals; D000927:Anticonvulsants / Q000097:blood*; D001667:Binding, Competitive; D011232:Chemical Precipitation; D002853:Chromatography, Liquid; D003124:Colorimetry; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007834:Lasers; D010634:Phenobarbital / Q000097:blood; D010672:Phenytoin / Q000097:blood; D011817:Rabbits
D000927:Anticonvulsants; D010672:Phenytoin; D010634:Phenobarbital