title: Bile salt alteration of ion transport across jejunal mucosa.
abstract: The effect of conjugated dihydroxy and trihydroxy bile salts on electrolyte transport across isolated rabbit jejunal mucosa was studied. Both taurochenodeoxycholic acid and taurocholic acid increased the short-circuit current (Isc) in bicarbonate-Ringer solution but not in a bicarbonate-free, chloride-free solution. Taurochenodeoxycholic acid was significantly more effective than taurocholic acid in increasing Isc. The presence of theophylline prevented the taurochenodeoxycholic acid- and taurocholic acid-induced increase in Isc. Transmural ion fluxes across jejunal mucosa demonstrated that 2 mM taurochenodeoxycholic acid decreased net Na+ absorption, increased net Cl- secretion and increased the residual flux (which probably represents HCO3- secretion). These studies support the hypothesis that cyclic AMP may be a mediator of intestinal electrolyte secretion.
mesh_terms: D000818:Animals; D001639:Bicarbonates / Q000378:metabolism; D001647:Bile Acids and Salts / Q000494:pharmacology*; D001693:Biological Transport, Active / Q000187:drug effects; D002635:Chenodeoxycholic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D002712:Chlorides / Q000378:metabolism*; D000242:Cyclic AMP / Q000378:metabolism; D004573:Electrolytes / Q000378:metabolism; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000033:anatomy & histology / Q000187:drug effects* / Q000378:metabolism; D007583:Jejunum; D008297:Male; D010743:Phospholipids / Q000494:pharmacology; D011817:Rabbits; D012964:Sodium / Q000378:metabolism*; D013654:Taurine / Q000031:analogs & derivatives; D013806:Theophylline / Q000494:pharmacology
chemical_list: D001639:Bicarbonates; D001647:Bile Acids and Salts; D002712:Chlorides; D004573:Electrolytes; D010743:Phospholipids; D002635:Chenodeoxycholic Acid; D013654:Taurine; D012964:Sodium; D013806:Theophylline; D000242:Cyclic AMP