Purification and characterization of equine infectious anemia virus.
EIA virus was purified from equine fetal kidney cell cultures by PEG-precipitation, two sucrose-gradient sedimentations (5-30 per cent) and (25 to 60 per cent) centrifugation, using the immunodiffusion test to follow the procedure. Purified EIA virus had a density (20 degrees C) of 1.162 and a sedimentation constant of S20w=656. electron microscopy revealed a particle of about 100 nm in diameter with a very flexible but usually spherical shape. The dense core may be at various locations inside the membrane bound particle.
D000818:Animals; D002462:Cell Membrane / Q000382:microbiology; D002499:Centrifugation, Density Gradient; D011232:Chemical Precipitation; D046508:Culture Techniques; D006736:Horses; D007181:Inclusion Bodies, Viral; D004860:Infectious Anemia Virus, Equine / Q000254:growth & development / Q000302:isolation & purification / Q000648:ultrastructure; D007668:Kidney / Q000196:embryology; D007962:Leukocytes