title: Epithelial basement membrane of bovine renal tubuli. Isolation and analysis of the carbohydrate chains.
abstract: The carbohydrate chains present in the tubular basement membrane of bovine kidney were studied. Digestion with collagenase followed with pronase resulted in a complete solubilization of the basement membrane. The different glycopeptides were purified by gel filtration and ion-exchange chromatography. Two kinds of carbohydrate chains could be characterized: oligosaccharides composed of glucosamine, mannose, galactose, fucose and sialic acid, and glucosylgalactose disaccharides. A very small portion of the oligosaccharide chains (ca. 4%) appeared to be free of sialic acid. The bulk of these chains contained sialic acid and fucose, although in small amounts. Only traces of galactosamine were found.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001485:Basement Membrane / Q000032:analysis*; D002417:Cattle; D005643:Fucose / Q000032:analysis; D005688:Galactosamine / Q000032:analysis; D005944:Glucosamine / Q000032:analysis; D006020:Glycopeptides / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis*; D006601:Hexoses / Q000032:analysis; D007684:Kidney Tubules / Q000032:analysis*; D003012:Microbial Collagenase; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis*; D011402:Pronase; D012794:Sialic Acids / Q000032:analysis
chemical_list: D000596:Amino Acids; D006020:Glycopeptides; D006023:Glycoproteins; D006601:Hexoses; D009005:Monosaccharides; D012794:Sialic Acids; D005643:Fucose; D005688:Galactosamine; D011402:Pronase; D003012:Microbial Collagenase; D005944:Glucosamine