The effect of low temperatures on the viability of Ehrlich ascites tumor cells.
By using rapid freezing-rapid thawing procedures on Ehrlich ascites tumor cells the authors succeeded in maintaining the viability of these cells, ascertained by two methods: a) trypan blue dye-exclusion test, and b) grafting in experimental animals of cells maintained for three days at -80 degrees C.
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D002286:Carcinoma, Ehrlich Tumor; D002470:Cell Survival; D002478:Cells, Cultured; D005615:Freezing; D051379:Mice; D009368:Neoplasm Transplantation