title: Stimulation of non-histone chromosomal protein synthesis in simian virus 40-infected simian cells.
abstract: The pattern of synthesis of non-histone chromosomal proteins in simian virus (SV) 40-infected African green monkey kidney cells was analyzed by polyacryl-amide gel electrophoresis to see whether the changes in chromosomal protein metabolism are involved in the viral-induced synthesis of cellular DNA and mRNA. During the prereplicative phase of infection, the rate of histone synthesis was decreased until 15 h postinfection, whereas that of non-histone protein synthesis was increased after 5 h postinfection and reached a maximum at 10 to 15 h postinfection when viral-induced synthesis of cellular DNA and mRNA began to be observed. Stimulation of non-histone protein synthesis was also observed in the infected cells treated with cytosine arabinoside and was dependent on the multiplicity of infection. Stimulation occurred in almost all species of non-histone proteins. These results suggest that the stimulation of non-histone protein synthesis is caused by an early SV40 function and occurs prior to the viral-induced synthesis of cellular DNA and mRNA. During the replicative phase of infection, a marked increase in the rate of synthesis was observed in the non-histone proteins with molecular weights of about 48,000, 35,000, and 23,000, which were subsequently found to be SV40 capsid proteins.
mesh_terms: D000818:Animals; D002460:Cell Line; D002875:Chromosomes / Q000378:metabolism*; D003561:Cytarabine / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D004591:Electrophoresis, Polyacrylamide Gel; D000882:Haplorhini; D006657:Histones / Q000096:biosynthesis; D007668:Kidney; D007930:Leucine; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000096:biosynthesis; D013539:Simian virus 40 / Q000254:growth & development*; D013997:Time Factors; D014316:Tritium; D014764:Viral Proteins / Q000096:biosynthesis*; D014779:Virus Replication
chemical_list: D006657:Histones; D012333:RNA, Messenger; D014764:Viral Proteins; D003561:Cytarabine; D014316:Tritium; D004247:DNA; D007930:Leucine