[Isolation of "pregnancy-zone" proteins using immuno absorbents and study of possible enzyme activities].
The purification of the pregnancy zone protein by means of immunoadsorbents is described. The pregnancy zone protein antibody was isolated from an absorbed rabbit antiserum and coupled with CNBr-activated sepharose. The pregnancy zone protein was isolated from pregnancy serum by the specific antibody cross-linked with sepharose. Contaminating serum proteins were eliminated by "inverse" immunoadsorption using antibodies against these proteins coupled with sepharose. An immunoelectrophoretically pure pregnancy zone protein was obtained. By means of a combination of immunoprecipitation and enzyme reaction in agar gel could be excluded that the pregnancy zone protein possesses activities of the following 11 enzymes: ceruloplasmin, leucine amino peptidase, alkaline phosphatase, carboxylic  esterase, lactate dehydrogenase, malate dehydrogenase, glycerophosphate dehydrogenase, glucose-6-phosphat-dehydrogenase, cholinesterase, acetyl cholinesterase and oxytocinase.
D000327:Adsorption; D000362:Agar; D000818:Animals; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism; D002570:Ceruloplasmin / Q000378:metabolism; D002802:Cholinesterases / Q000378:metabolism; D010122:Cystinyl Aminopeptidase / Q000378:metabolism; D004798:Enzymes / Q000378:metabolism*; D005260:Female; D005947:Glucose / Q000378:metabolism; D006023:Glycoproteins / Q000302:isolation & purification*; D007931:Leucyl Aminopeptidase / Q000378:metabolism; D014442:Monophenol Monooxygenase / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism; D010455:Peptides / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D011247:Pregnancy; D011817:Rabbits; D012685:Sepharose / Q000378:metabolism
D004798:Enzymes; D006023:Glycoproteins; D010455:Peptides; D000362:Agar; D012685:Sepharose; D010088:Oxidoreductases; D014442:Monophenol Monooxygenase; D002570:Ceruloplasmin; D002265:Carboxylic Ester Hydrolases; D002802:Cholinesterases; D010744:Phosphoric Monoester Hydrolases; D007931:Leucyl Aminopeptidase; D010122:Cystinyl Aminopeptidase; D005947:Glucose
Isolierung des Proteins der "pregnancy zone" mit Hilfe von Immunadsorbentien und Untersuchungen zur Frage möglicher enzymatischer Aktivitäten