The effects of a diphosphonate and dietary calcium on the metabolism of vitamin D3 (cholecalciferol) in the chick.
1. Vitamin D-deficient chicks, maintained on a diet adequate in calcium and treated with ethane-1-hydroxy-1,1-diphosphonate for 2 days before a single oral dose of cholecalciferol (vitamin D3), converted the vitamin into 24,25-dihydroxycholecalciferol instead of into the normal metabolite 1,25-dihydroxycholecalciferol. 2. This inhibition of the renal 1-hydroxylase disappeared on withdrawal of the diphosphonate. 3. Kidneys from chicks given diphosphonate for 12 days converted 25-hydroxycholecalciferol into 24,25-dihydroxycholecalciferol on incubation in vitro. 4. The inhibition of the 1-hydroxylase was markedly accelerated by treating the birds with cholecalciferol. 5. No inhibition of renal 1-hydroxylation was observed in birds maintained on a diet low in calcium. 6. A possible mechanism producing this effect is discussed.
D000818:Animals; D002118:Calcium / Q000097:blood; D002136:Calcium, Dietary; D002645:Chickens; D002762:Cholecalciferol / Q000378:metabolism* / Q000494:pharmacology; D004100:Dihydroxycholecalciferols / Q000097:blood / Q000378:metabolism; D012968:Etidronic Acid / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000378:metabolism; D007668:Kidney / Q000201:enzymology / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000378:metabolism; D009943:Organophosphorus Compounds / Q000494:pharmacology*; D014808:Vitamin D Deficiency / Q000378:metabolism
D002136:Calcium, Dietary; D004100:Dihydroxycholecalciferols; D009943:Organophosphorus Compounds; D002762:Cholecalciferol; D006899:Mixed Function Oxygenases; D012968:Etidronic Acid; D002118:Calcium