[Localization of N-acetyltransferases in sinusoidal liver cells. Effect of zymosan on the acetylation of sulfamethazine and isoniazid in the rat and in isolated perfused liver].
N-acetyltransferase (NAT) have been demonstrated to be localized in sinusoïdal cells and not in hepatocytes. Acetylation activities increased after pretreatment with Zymosan (40 mg/kg, i.v., 24 h before phenotyping) as well in vivo and in isolated perfused liver and in cytosolic fractions prepared from several rats organs. These data favor the localization of NAT-activity in reticulo-endothelial system. Increased protein levels in liver, gut, spleen and lungs cytosolic fractions (100 000 g, 120 mn) 48 h after Zymosan administration, suggest and inducing effect of this drug on NAT activity.
D000107:Acetylation; D000123:Acetyltransferases / Q000378:metabolism*; D000818:Animals; D066298:In Vitro Techniques; D007538:Isoniazid / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010477:Perfusion; D051381:Rats; D013418:Sulfamethazine / Q000378:metabolism*; D015054:Zymosan / Q000494:pharmacology*
D013418:Sulfamethazine; D015054:Zymosan; D000123:Acetyltransferases; D007538:Isoniazid
Localisation des N-acétyltransférases dans les cellules sinusoïdales hépatiques. Influence du zymosan sur l'acétylation de la sulfaméthazine et de l'isoniazide chez le rat et dans le foie isolé perfusé.