title: On the use of the spin labeling technique in the study of erythrocyte membranes.
abstract: ESR spectra and scanning electron micrographs of human erythrocytes spin labeled with the conventional stearic acid nitroxide substituted at the 5-position have been obtained over a range of label-to-lipid ratios. While morphological changes as previously reported (Bieri, V. G., Wallach, D. F. H. and Lin, P. S. (1974) Proc. Natl. Acad. Sci. U.S. 71, 4797-4801) are reproduced, it is shown that at label-to-lipid ratios of 1:10 or less the basic ESR spectrum is not significantly affected. At low label concentrations the spin labeling technique is a viable one and can be used to investigate membrane properties.
mesh_terms: D001665:Binding Sites; D002458:Cell Fractionation; D002462:Cell Membrane / Q000648:ultrastructure*; D004578:Electron Spin Resonance Spectroscopy; D004912:Erythrocytes / Q000648:ultrastructure*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D013113:Spin Labels; D013229:Stearic Acids / Q000097:blood
chemical_list: D013113:Spin Labels; D013229:Stearic Acids