title: The culture of skin. A review of theories and experimental methods.
abstract: Two main criticisms can be leveled against the standard methods of skin culture: they are poorly quantifiable and the cultured cell populations are heterogeneous. A new technique based mainly on enzymatic dissociation allows specific cell types to be extracted from the skin before cultivation. In this way, separate cultures of epidermal keratinocytes and dermal fibroblasts can be obtained from the same piece of skin. These purified systems have been used to study the kinetics of epidermal cell growth and to quantify the effect of various chemically defined substances on the growth and differentiation of keratinocytes. With further refinements in technique, purified populations of melanocytes can be extracted. The co-culture of pigmented melanocytes with albino keratinocytes has been proposed as a model to study pigment donation in vitro. The usual organ culture technique, including the use of large explants of skin immersed in the culture fluid, has been modified to show that adult human skin partially regenerates in vitro and that mitotic activity goes on for months in the regenerated epidermis. The use of nucleic acid hybridization techniques, combined with skin cell cultures from human tumors, opens new avenues of research on human cancer.
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D003584:Cytological Techniques; D004273:DNA, Neoplasm; D004279:DNA, Viral; D003864:Depression, Chemical; D006801:Humans; D008938:Mitosis / Q000187:drug effects; D009924:Organ Culture Techniques; D011565:Psoriasis / Q000473:pathology; D012867:Skin / Q000166:cytology* / Q000378:metabolism; D012878:Skin Neoplasms / Q000473:pathology
chemical_list: D000596:Amino Acids; D004273:DNA, Neoplasm; D004279:DNA, Viral; D003994:Bucladesine