2'-O-methylated oligonucleotides in ribosomal 18S and 28S RNA of a mouse hepatoma, MH 134.
Simple two-dimensional thin-layer chromatography was found to be useful for the separation of sugar methylated dinucleotides in RNA. Of the 16 possible sequences of the type Nm-Np, 15 were separated and all the sequences were determined. In a mouse hepatoma, MH 134, the levels of the sugar methylation in the 18S and 28S RNA molecules were 17-18 and 11-12 per 1000 nucleotides, respectively. Thus, 18s RNA contained approximately 35 2'-O-methylated dinucleotides and 28S RNA approximately 60 2'-O-methylated dinucleotides. The pattern of distribution was also distinct between these two molecules. Two 2'-O-methylated trinucleotides were identified in the 28S RNA with the sequences Um-Gm-Up and Um-Gm-psip. A unique 2'-O-methylated tetranucleotide was present also in the 28S RNA, the sequence of which was Am-Gm-Cm-Ap. The 5'-terminal nucleotides of both 18S and 28S RNA were obtained as nucleoside 3',5'-diphosphates (pNp) in the trinucleotide fraction of the RNase T2 digest. The 5'-termimi of 18S and 28S RNA were pUp and pCp, respectively, and found to be almost homogeneous.
D000469:Alkaline Phosphatase; D000818:Animals; D001483:Base Sequence; D006528:Carcinoma, Hepatocellular / Q000032:analysis*; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D004926:Escherichia coli / Q000201:enzymology; D008113:Liver Neoplasms; D051379:Mice; D009374:Neoplasms, Experimental / Q000032:analysis; D009841:Oligonucleotides / Q000032:analysis*; D010727:Phosphoric Diester Hydrolases; D012334:RNA, Neoplasm / Q000032:analysis*; D012335:RNA, Ribosomal / Q000032:analysis; D012260:Ribonucleases; D012265:Ribonucleotides / Q000032:analysis; D012270:Ribosomes / Q000032:analysis; D012910:Snake Venoms
D009841:Oligonucleotides; D012334:RNA, Neoplasm; D012335:RNA, Ribosomal; D012265:Ribonucleotides; D012910:Snake Venoms; D012260:Ribonucleases; D000469:Alkaline Phosphatase; D010727:Phosphoric Diester Hydrolases