Origin of lymphoid lines established from mixed cultures of cord-blood lymphocytes and explants from infectious mononucleosis, Burkitt lymphoma and healthy donors.
Lymphocytes were explanted from EBV-seropositive donors including peripheral blood of infectious mononucleosis patients, healthy donors and EBV-genome-carrying cells from Burkitt lymphoma (BL) biopsies or nude mouse-passaged, BL-biopsy-derived lines. The explanted cells were mixed with fresh cord-blood lymphocytes from mice of the opposite sex. In all categories of derived lines, cord-blood cell progeny was predominant, as judged by the sex marker and other associated markers. Only one BL biopsy line, serially passaged in nude mice, gave rise to a monoclonal lymphoma line.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002051:Burkitt Lymphoma / Q000382:microbiology; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D002471:Cell Transformation, Neoplastic; D002999:Clone Cells; D005260:Female; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis; D007244:Infectious Mononucleosis / Q000097:blood; D008214:Lymphocytes / Q000276:immunology / Q000382:microbiology; D008297:Male; D051379:Mice; D008819:Mice, Nude; D014470:Umbilical Cord
D000956:Antigens, Viral; D007136:Immunoglobulins