RNA metabolism of murine leukemia virus II. Endogenous virus-specific RNA in the uninfected BALB/c cell line JLS-V9.
Type C virus-specific RNA sequences of BALB/c endogenous virus were detected in JLS-V9 cells (an uninfected BALB/c derived line) by annealing cell RNA with 3-H-labeled virus-specific DNA. Endogenous viruses used in preparing the 3-H-labeled DNA (mostly xenotropic) was prepared from JLS-V9 cells induced to produce virus with iododeoxyuridine. In whole-cell extracts, two virus-specific RNA species, 38S and 27S, were detected. No 60 to 70S virus-specific RNA was found. The same two species of virus-specific RNA were observed in isolated cytoplasmic RNA and in cytoplasmic RNA selected for polyadenylic acid-containing species by binding and elution from oligo(dT) cellulose. Very little, if any, of the virus-specific RNA was active as messenger RNA on polyribosomes. No virus-specific RNA transcribed from genes coding for the BALB/c endogenous N-tropic virus was detected, since 3-H-labeled DNA prepared from endogenous N-tropic virus did not hybridize measurably with JLS-V9 RNA.
D000818:Animals; D001483:Base Sequence; D001854:Bone Marrow Cells; D002460:Cell Line; D002478:Cells, Cultured / Q000187:drug effects / Q000382:microbiology; D002501:Centrifugation, Zonal; D003593:Cytoplasm / Q000032:analysis; D007065:Idoxuridine / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D009693:Nucleic Acid Hybridization; D011132:Polyribosomes / Q000032:analysis; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000032:analysis*; D012190:Retroviridae
D012367:RNA, Viral; D007065:Idoxuridine