title: Distribution of murine type B and type C viral nucleic acid sequences in template active and template inactive chromatin.
abstract: Template active chromatin and template inactive chromatin have been fractionated from mouse cells infected with the Moloney strain of murine leukemia virus. In vivo the cells produce abundant Rna homologous to Moloney leukemia virus, but do not produce either globin mRNA or RNA homologous to type B mouse mammary tumor virus. The DNA extracted from the template active chromatin or template inactive chromatin contained equal amounts of sequences homologous to Moloney type C virus, to type B virus, or to globin mRNA. The results are discussed with regard to the in vivo structure of chromatin and the difficulties in fractionating chromatin in vitro.
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D002843:Chromatin / Q000032:analysis*; D002852:Chromatography, Ion Exchange; D004247:DNA / Q000032:analysis; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D005347:Fibroblasts; D005914:Globins; D008324:Mammary Tumor Virus, Mouse / Q000032:analysis*; D051379:Mice; D008979:Moloney murine leukemia virus / Q000032:analysis*; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis*; D013010:Sonication; D013698:Templates, Genetic; D014316:Tritium
chemical_list: D002843:Chromatin; D012333:RNA, Messenger; D012367:RNA, Viral; D014316:Tritium; D005914:Globins; D004247:DNA; D012321:DNA-Directed RNA Polymerases