S-adenosylmethionine:protein methyltransferases in hepatomas.
Protein methylase III (S-adenosylmethionine:proteinlysine methyltransferase; ED 2.1.1.25) and protein methylase I (S-adenosylmethionine:protein-arginine methyltransferase; EC 2.1.1.23) activities were examined in isolated nuclei and cytosol fraction, respectively, from various hepatomas with different growth rates. The enzyme activities of both enzymes paralleled the rates of tumor growth in fast- and moderately growing hepatomas. The parallelism was more evident with protein methylase I than with protein methylase III. While protein methylase III activity was elevated in the fast- to moderately growing hepatomas, the enzyme that is responsible for demethylating proteins, epsilon-alkyllysinase (epsilon-slkyl-L-lysine:oxygen oxidoreductase; EC 1.5.3.4), had an inverse relationship to the rate of tumor growth, thus suggesting a possible physiological antagonism. When isolated rat liver nuclei were methylated in vitro with S-adenosyl-L-[methyl-14 C]methionine as methyl donor, H2SO4-insoluble protein and histones had almost equal amounts of methyl-14 C incorporated. However, amino acid analysis revealed that methylated arginines are the predominant form of radioactivity in the H2SO4-insoluble protein (product of protein methylase I), while methylated lysines are the major methylated amino acids in the histones (product of protein methylase III). Furthermore, the hydrolysate of the H2SO4-insoluble protein showed four unknown radioactivity peaks on the amino acid analyzer in addition to the known methylated arginine and lysine derivatives.
D000225:Adenine; D000818:Animals; D001120:Arginine; D006528:Carcinoma, Hepatocellular / Q000201:enzymology* / Q000473:pathology; D002467:Cell Nucleus / Q000201:enzymology; D003600:Cytosol / Q000201:enzymology; D006657:Histones / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D008113:Liver Neoplasms / Q000201:enzymology* / Q000473:pathology; D008239:Lysine; D008715:Methionine; D008780:Methyltransferases / Q000378:metabolism*; D009374:Neoplasms, Experimental / Q000201:enzymology / Q000473:pathology; D010089:Oxidoreductases, N-Demethylating; D051381:Rats; D013464:Sulfuric Acids
D006657:Histones; D013464:Sulfuric Acids; D001120:Arginine; D008715:Methionine; D010089:Oxidoreductases, N-Demethylating; D008780:Methyltransferases; D000225:Adenine; D008239:Lysine