5'-terminal structure of poliovirus polyribosomal RNA is pUp.
Poliovirus RNA purified from virus-specific polyribosomes does not contain m7G in a 5'-5'-pyrophosphate  linkage at its 5'-end. The only potential 5'-end found in ribonuclease digests of this RNA is pUp, which is present in a yield of 1 mole/mole of poliovirus RNA. We conclude that a 5'-terminal m7G is not required for translation of at least one RNA species in animal cells.
D001483:Base Sequence; D004590:Electrophoresis, Paper / Q000379:methods; D017955:Poliovirus / Q000032:analysis*; D011132:Polyribosomes / Q000032:analysis*; D012333:RNA, Messenger / Q000032:analysis* / Q000302:isolation & purification; D012367:RNA, Viral / Q000032:analysis* / Q000302:isolation & purification; D014500:Uracil Nucleotides / Q000032:analysis*
D012333:RNA, Messenger; D012367:RNA, Viral; D014500:Uracil Nucleotides