Effect of thymidine on poly(ADP-ribosyl)ation in vivo.
The addition of thymidine as well as nicotinamide to isolated nuclei resulted in a strong inhibition of poly(ADP-ribosyl)ation, whereas that of hydroxyurea and amethopterin has essentially no effect. The nuclei isolated from the cells immediately after release from thymidine synchronization exhibited a significantly increased activity of poly(ADP-ribosyl)ation. Thereafter, the fluctuation pattern of the activity of poly(ADP-ribosyl)ation in isolated nuclei during the cell cycle was essentially the same as in the case of hydroxyurea synchronization. The activity of poly(ADP-ribosyl)ation in isolated nuclei after treatment with thymidine in vivo increased with the treatment time. The time-dependent increase was also evident in the case of nicotinamide treatment. Little increase in the activity was observed in hydroxyurea and amethopterin treatment. When poly(ADP-ribose) polymerase was extracted from the nuclei isolated from the cells which were pretreated with each of the four compounds, there was no significant difference in the amount among these compounds. The reason for the increase in the poly(ADP-ribosyl)ation in vitro by the in vivo treatment with thymidine is discussed.
D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D004261:DNA Replication; D004273:DNA, Neoplasm / Q000096:biosynthesis; D006367:HeLa Cells / Q000378:metabolism; D006801:Humans; D006918:Hydroxyurea / Q000494:pharmacology; D007700:Kinetics; D008727:Methotrexate / Q000494:pharmacology; D009536:Niacinamide / Q000494:pharmacology; D009702:Nucleoside Diphosphate Sugars / Q000096:biosynthesis*; D011064:Poly Adenosine Diphosphate Ribose / Q000096:biosynthesis*; D013936:Thymidine / Q000494:pharmacology*
D004273:DNA, Neoplasm; D009702:Nucleoside Diphosphate Sugars; D009536:Niacinamide; D011064:Poly Adenosine Diphosphate Ribose; D013936:Thymidine; D006918:Hydroxyurea; D008727:Methotrexate