Azaguanine-resistant mutants induced by several mutagens in a neurospora heterokaryon.
This paper reports the development of a new system for the detection of forward mutations in a heterokaryon of Neurospora crassa. This system, designed to detect a wide variety of genetic alterations, is based upon the detection of 8-azaguanine-resistant mutants in an adenine-requiring heterokaryon carrying the aza-3 gene. Induction of mutations in the aza-3 gene was detected after treatment of conidia with ultraviolet light, X-rays, ethyl methanesulfonate (EMS), or the acridine mustard, ICR-170. The pattern of appearance of mutant colonies and various factors in mutant selection are discussed.
D000166:Acridines / Q000494:pharmacology; D000225:Adenine / Q000378:metabolism; D001375:Azaguanine / Q000494:pharmacology*; D002467:Cell Nucleus; D002874:Chromosome Mapping; D004352:Drug Resistance, Microbial; D005796:Genes; D006579:Heterozygote; D008698:Mesylates / Q000494:pharmacology; D009153:Mutagens / Q000494:pharmacology*; D009154:Mutation; D009491:Neurospora; D009492:Neurospora crassa / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D011831:Radiation Genetics; D013997:Time Factors; D014466:Ultraviolet Rays; D014965:X-Rays
D000166:Acridines; D008698:Mesylates; D009153:Mutagens; D000225:Adenine; D001375:Azaguanine