Recovery of murine leukemia virus from large volumes of freshly harvested culture fluids by using a single density gradient.
Concentration and purification of murine leukemia virus for use as complement fixation antigens was accomplished by using a single density gradient in the model RK ultracentrifuge.
D000818:Animals; D000956:Antigens, Viral; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D003168:Complement Fixation Tests / Q000592:standards; D005557:Formaldehyde; D006041:Goats / Q000276:immunology; D007106:Immune Sera; D009052:Leukemia Virus, Murine / Q000302:isolation & purification* / Q000648:ultrastructure; D051379:Mice / Q000276:immunology; D008815:Mice, Inbred Strains; D008854:Microscopy, Electron; D051381:Rats / Q000276:immunology; D013056:Spectrophotometry, Ultraviolet; D013395:Sucrose; D013501:Surface-Active Agents; D014764:Viral Proteins
D000956:Antigens, Viral; D007106:Immune Sera; D013501:Surface-Active Agents; D014764:Viral Proteins; D005557:Formaldehyde; D013395:Sucrose