Peripheral human T cells sensitized in mixed leukocyte culture synthesize and express Ia-like antigens.
We have studied the modulation of Ia-like antigens on the surface membrane of human T cells responding in a one-way mixed leukocyte culture. A heterologous antiserum, (anti-p23,30), which is specific to HLA-D-related antigens and which is unreactive with normal peripheral T cells or thymocytes, was found to bind significantly to all T cells transformed in mixed leukocyte culture (MLC) as determined by indirect immunofluorescence on a fluorescence-activated cell sorter 1. Furthermore, cytotoxic T cells responsible for cell-mediated lympholysis were shown to react with anti-p23,30, whereas their unactivated progenitors did not. Immunoprecipitation and sodium dodecyl sulfate polyacrylamide gel electrophoresis of a radioactive 29,000 and 34,000 dalton complex from MLC-primed T cells labeled with [35S]methionine indicated that allosensitized T cells synthesized these HLA-D-related antigens.
D000954:Antigens, Surface / Q000235:genetics; D002478:Cells, Cultured; D008040:Genetic Linkage; D006680:HLA Antigens / Q000235:genetics; D006801:Humans; D007519:Isoantigens / Q000235:genetics; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008970:Molecular Weight; D013601:T-Lymphocytes / Q000276:immunology*
D000954:Antigens, Surface; D006680:HLA Antigens; D007519:Isoantigens