title: Sporadic bovine meningo-encephalitis-isolation of a paramyxovirus.
abstract: Isolation of a viral agent (107) directly from brain explants of a 15-month-old heifer with symptoms of a sporadic encephalomyelitis is described. The virus shares properties with the paramyxovirus family. It grows in a variety of cell cultures from different species, and induces nuclear and cytoplasmic inclusion bodies in infected cells. Nucleocapsids measuring 17 nm in diameter were found in the nucleus and cytoplasm of these cells when studied electron microscopically, thus indicating a close relationship of the agent to the measles-distemper-rinderpest group. No infectious virus was released from infected cells, although alignment of nucleocapsids was observed beneath the cell membrane, and no hemagglutinating activity could be detected with the methods employed. The 107 agent was compared serologically with parainfluenza viruses type 1, 2 and 3, simian virus 5, mumps and Newcastle disease virus (NDV), two bovine respiratory syncytial viruses and measles/subacute sclerosing panencephalitis, distemper and rinderpest viruses, always using 107 virus infected CV1 cells and antiserum of the different viruses in indirect FA tests. Positive FA reactions were observed only with two sera obtained from SSPE patients with high antibody titer to SSPE virus, and with one rabbit-anti-rinderpest serum. The titers of these sera to 107 virus, however, were significantly lower than those against homologous viruses. Five out of 9 sera from randomly selected healthy cattle showed antibody titers between 1:10 and 1:80 to 107 virus in FA tests. The significance of these results is discussed with respect to the epidemiology of SSPE in children and its possible implication with rinderpest in Europe.
mesh_terms: D000818:Animals; D000914:Antibodies, Viral; D000956:Antigens, Viral / Q000032:analysis; D001921:Brain / Q000382:microbiology / Q000473:pathology; D002213:Capsid / Q000032:analysis; D002417:Cattle; D002460:Cell Line; D002467:Cell Nucleus / Q000648:ultrastructure; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D005260:Female; D005455:Fluorescent Antibody Technique; D006382:Hemadsorption; D006386:Hemagglutination Tests; D007118:Immunoassay; D002479:Inclusion Bodies / Q000648:ultrastructure; D008590:Meningoencephalitis / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D009474:Neurons / Q000648:ultrastructure; D010252:Paramyxoviridae / Q000276:immunology / Q000302:isolation & purification* / Q000502:physiology
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral