[Spin label study of microviscosity and structural transitions in cell membrane lipids and proteins. I. Heat-induced structural changes in cell nuclei of mouse organs in normal conditions].
With the help of weakly bound spin probes the effect of temperature on structural transitions of protein and lipid components in the nuclei membranes of different organs of intact mice has been studied. Microviscosity of lipids in the membrane is found to differ sharply from proteins. Found by both probes breaks on Arrhenius curve of the correlation time dependence on reverse temperature within the temperature range of 25degreesC--27degreesC and 40degreesC-43degreesC seem to be connected with structural reconstructions of the whole membrane. The results obtained according to the behaviour of probes are identical for the nuclei of different organs.
D000818:Animals; D002462:Cell Membrane; D002467:Cell Nucleus; D003497:Cyclic N-Oxides; D004578:Electron Spin Resonance Spectroscopy; D006358:Hot Temperature; D007668:Kidney / Q000166:cytology*; D008055:Lipids; D008099:Liver / Q000166:cytology*; D051379:Mice; D008968:Molecular Conformation; D011506:Proteins; D013113:Spin Labels; D014783:Viscosity
D003497:Cyclic N-Oxides; D008055:Lipids; D011506:Proteins; D013113:Spin Labels
Issledovanie mikroviazkosti i strukturnykh perekhodov v lipidakh i belkakh kletochnykh membran metodom spinovykh zondov. I. Termoindutsirovannye strukturnye izmeneniia v iadrakh kletok razlichnykh organov mysheĭ v norme