The generation of O2-by the interaction of the hemolytic agent, phenylhydrazine, with human hemoglobin.
Phenylhydrazine reacts with adult oxy- or methemoglobin in the presence of molecular oxygen to generate O2-. The mechanism of the reaction apparently differs in each case. The generation of O2- is monitored by following the reduction of ferricytochrome c or the oxidation of epinephrine.
D000838:Anions; D003574:Cytochrome c Group / Q000378:metabolism; D004837:Epinephrine / Q000378:metabolism; D006454:Hemoglobins / Q000378:metabolism*; D006801:Humans; D008706:Methemoglobin / Q000378:metabolism; D010100:Oxygen; D010108:Oxyhemoglobins / Q000378:metabolism; D010659:Phenylhydrazines / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism
D000838:Anions; D003574:Cytochrome c Group; D006454:Hemoglobins; D010108:Oxyhemoglobins; D010659:Phenylhydrazines; D008706:Methemoglobin; D013482:Superoxide Dismutase; D010100:Oxygen; D004837:Epinephrine