Growth on D-arabitol of a mutant strain of Escherichia coli K12 using a novel dehydrogenase and enzymes related to L-1,2-propanediol and D-xylose metabolism.
Escherichia coli K12 cannot grow on D-arabitol, L-arabitol, ribitol or xylitol (Reiner, 1975). Using a mutant of E. coli K12 (strain 3; Sridhara et al., 1969) that can grow on L-1,2-propanediol, a second-stage mutant was isolated which can utilize D-arabitol as sole source of carbon and energy for growth. D-Arabitol is probably transported into the bacteria by the same system as that used for the transport of L-1,2-propanediol. The second-stage mutant constitutively synthesizes a new dehydrogenase, which is not present in the parent strain 3. This enzyme, whose native substrate may be D-galactose, apparently dehydrogenates D-arabitol to D-xylulose, and its structural gene is located at 68.5 +/- 1 min on the E. coli genetic map. D-Xylulose is subsequently catabolized by the enzymes of the D-xylose metabolic pathway.
D002238:Carbohydrate Epimerases / Q000378:metabolism; D002474:Cell-Free System; D004926:Escherichia coli / Q000201:enzymology / Q000254:growth & development* / Q000378:metabolism; D005690:Galactose / Q000378:metabolism; D005796:Genes; D009154:Mutation; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D010770:Phosphotransferases / Q000378:metabolism; D011409:Propylene Glycols / Q000378:metabolism*; D013237:Stereoisomerism; D013402:Sugar Alcohols / Q000378:metabolism*; D014994:Xylose / Q000378:metabolism*; D014996:Xylulose / Q000096:biosynthesis
D011409:Propylene Glycols; D013402:Sugar Alcohols; D014996:Xylulose; D014994:Xylose; D009247:NADH, NADPH Oxidoreductases; D010770:Phosphotransferases; D002238:Carbohydrate Epimerases; D005690:Galactose