The modified nucleosides of tRNAs. II. Synthesis of 2'-O-methylcytidylyl (3'-5') cytidine.
The synthesis of 2'-O-methylcytidylyl (3'-5')cytidine by the triester method using as protecting groups, 2,2,2-trichloroethyl for phosphate hydroxyl group, p-chlorophenyoxyacetyl for 5-hydroxyl group, methoxymethylidene for 2',3'-cis-diol system, and benzoyl for the exo-amino group of cytidine is presented. The obtained product was characterised by UV, electrophoresis, chromatography and an enzymatic digestion.
D002854:Chromatography, Paper; D002855:Chromatography, Thin Layer; D003562:Cytidine; D003597:Cytosine Nucleotides / Q000138:chemical synthesis*; D004586:Electrophoresis; D008745:Methylation; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D012343:RNA, Transfer / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet
D003597:Cytosine Nucleotides; D003562:Cytidine; D012343:RNA, Transfer; D010727:Phosphoric Diester Hydrolases