title: [Plasmids controlling the resistance to several antibiotics in C. perfringens type A, strain 659].
abstract: A strain of C. perfringens type A, isolated from a patient, was found to be resistant to four antibiotics: tetracycline (Tet), chloramphénicol (Chl), erythromycin (Ero) and clindamycin (Cli). Clones resistant to only two drugs (Tet-Chl or Ero-Cli), or sensitive to all drugs were found in cultures of the wild-type strain treated by acridine dyes or ethidium bromide. DNA analysis by equilibrium centrifugation confirmed that the original strain contains two resistance plasmids, one called Rip 401 (Tet-Chl) and the other Rip 402 (Ero-Cli), which represent respectively 1.8 +/- 0.2% and 2.3 +/- 0.2% of the total amount of DNA in this strain of Cl. perfrigens.
mesh_terms: D002500:Centrifugation, Isopycnic; D002701:Chloramphenicol / Q000494:pharmacology; D002981:Clindamycin / Q000494:pharmacology; D003016:Clostridium perfringens / Q000187:drug effects*; D004247:DNA / Q000032:analysis; D004352:Drug Resistance, Microbial; D004917:Erythromycin / Q000494:pharmacology; D004996:Ethidium / Q000494:pharmacology; D007933:Leucomycins / Q000494:pharmacology; D008034:Lincomycin / Q000494:pharmacology; D010957:Plasmids; D013752:Tetracycline / Q000494:pharmacology
chemical_list: D007933:Leucomycins; D002981:Clindamycin; D004917:Erythromycin; D002701:Chloramphenicol; D004247:DNA; D008034:Lincomycin; D004996:Ethidium; D013752:Tetracycline
vernacular_title: Nature plasmidique de la résistance à plusieurs antibiotiques chez C. perfringens type A, souche 659