The coagulation of insoluble and basic proteins from rat siminal vesicle secretion with vesiculase: influence of collagenase-like peptidase from rat testis.
Proteins from the secretion of rat seminal vesicles were fractionated to yield (i) insoluble protein, (ii) basic proteins (soluble in the presence of urea) and (iii) neutral plus acidic proteins. The first two are necessary for the formation in vitro of a coagulum which is very similar to the copulatory plug. Collagenase-like peptidase from rat testis degrades both protein fractions and prevents coagulum formation.
D000577:Amides; D000818:Animals; D007611:Aprotinin / Q000494:pharmacology; D011232:Chemical Precipitation; D003307:Copulation; D004492:Edetic Acid / Q000494:pharmacology; D004589:Electrophoresis, Disc; D008297:Male; D003012:Microbial Collagenase / Q000378:metabolism*; D008854:Microscopy, Electron; D009584:Nitrogen / Q000032:analysis; D011392:Proline / Q000032:analysis; D011506:Proteins / Q000032:analysis / Q000378:metabolism*; D051381:Rats; D012661:Semen; D012669:Seminal Vesicles / Q000032:analysis / Q000378:metabolism*; D013737:Testis / Q000201:enzymology*; D014166:Transferases / Q000378:metabolism*
D000577:Amides; D011506:Proteins; D007611:Aprotinin; D011392:Proline; D004492:Edetic Acid; D014166:Transferases; D003012:Microbial Collagenase; D009584:Nitrogen