The effect of influenza virus infection on the agglutinability by concanavalin A of polyoma-transformed BHK-21 cells.
Polyoma-transformed (Py-transformed) BHK-21 cells are agglutinated more readily by Concanavalin A (Con A) after they are infected with influenza virus; this agglutination was only slightly affected by fixation with glutaraldehyde or colchicine treatment. It is suggest that the mechanism is not dependent on membrane fluidity. The same amounts of 125i-labelled Con A were bound after infection with influenza virus and after glutaraldehyde fixation with or without colchicine treatment: it seems therefore that new receptors for Con A are not expressed between 90 and 600 minutes after these cells are infected with influenza virus.
D000371:Agglutination / Q000187:drug effects*; D001666:Binding Sites, Antibody; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D003078:Colchicine / Q000494:pharmacology; D003208:Concanavalin A / Q000494:pharmacology*; D004912:Erythrocytes / Q000276:immunology; D005976:Glutaral / Q000494:pharmacology; D006382:Hemadsorption; D006801:Humans; D007457:Iodine Radioisotopes; D009975:Orthomyxoviridae / Q000254:growth & development*; D011120:Polyomavirus
D007457:Iodine Radioisotopes; D003208:Concanavalin A; D003078:Colchicine; D005976:Glutaral