[Role of phospholipids in the generation of membrane potentials by proteoliposomes].
Closed protein-phospholipid particles (proteoliposomes), obtained by self-assembly method, are capable to generate and to maintain the membrane potential in the case if their protein complex is represented by: a) a complex of mitochondrial ATPase; b) a complex of cytochrome oxidase and cytochrome c and c) bacteriorhodopsin from Halobacterium halobium; and their phospholipid component is represented by phosphatidylethanolamine or by a mixture of mitochondrial phospholipids. Only cytochromoxidase and bacteriorhodopsin (but not ATPase) proteoliposomes with phosphatidylserine are active. Cardiolipin also is not active in experiments with ATPase. Phosphatidylcholine produces in all the cases proteoliposomes incapable of maintaining the membrane potential. It is concluded that the inefficiency of phosphatidylcholine in the formation of proteoliposomes, generating the membrane potential, is due to the impossibility of obtaining closed membrane forms with a high electric resistance. The inefficiency of phosphatidylserine and cardiolipine, in the case of ATPase protein component of proteoliposomes, may be due to a specific requirement of this generator of the membrane potential in phosphatidylethanolamine.
D000251:Adenosine Triphosphatases / Q000502:physiology; D001436:Bacteriorhodopsins / Q000502:physiology; D003580:Cytochromes / Q000502:physiology; D004553:Electric Conductivity; D003576:Electron Transport Complex IV / Q000502:physiology; D006217:Halobacterium / Q000502:physiology; D008074:Lipoproteins / Q000502:physiology*; D008081:Liposomes / Q000502:physiology*; D008564:Membrane Potentials; D008566:Membranes / Q000502:physiology; D008928:Mitochondria / Q000502:physiology; D010714:Phosphatidylethanolamines / Q000502:physiology; D010743:Phospholipids / Q000502:physiology*
D003580:Cytochromes; D008074:Lipoproteins; D008081:Liposomes; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D001436:Bacteriorhodopsins; D003576:Electron Transport Complex IV; D000251:Adenosine Triphosphatases
O roli fosfolipidov v generatsii menbrannogo potentsiala proteoliposomani