Primary structure of human fibrinogen and fibrin. Structural studies on NH2-terminal part of B beta chain.
The cyanogen bromide fragment, N-DSK, containing the NH2-terminal portions of the three chains of fibrinogen, was found to exist in dimeric and polymeric forms. These different forms gave rise to identical chain fragments on reduction and alkylation. The B beta chain of N-DSK from fibrinogen and the beta chain of N-DSK from fibrin were isolated and characterized. The B beta chain fragment has a blocked NH2-terminal residue, and fibrinopeptide B is released on digestion with thrombin. The beta chain fragment has glycine as NH2-terminal residue. The molecular weight of the B beta chain fragment is 12200 as determined by ultracentrifugal analysis. Gel electrophoresis in sodium dodecyl sulphate gave the molecular weights of 14000 and 13000 for the B beta chain and beta chain fragments, respectively. The NH2-terminal B beta chain fragment consists of 118 amino acid residues and the beta chain fragment of 104 residues. The amino acid sequence of beta chain fragment is identical to B beta chain fragment except for the fibrinopeptide B portion. The isolation of a B beta-related fragment (B beta +), with a molecular weight of 30000, is also reported. The presence of B beta + was explained on the basis of incomplete cleavage at the Met-118 residue during treatment with cyanogen bromide. Some functional aspects of the B beta chain fragment are discussed.
D000595:Amino Acid Sequence; D002241:Carbohydrates / Q000032:analysis; D002268:Carboxypeptidases; D002918:Chymotrypsin; D003488:Cyanogen Bromide; D005337:Fibrin; D005340:Fibrinogen; D005341:Fibrinolysin; D006801:Humans; D005779:Immunodiffusion; D046911:Macromolecular Substances; D010196:Pancreatic Elastase; D010446:Peptide Fragments / Q000032:analysis; D013820:Thermolysin; D013917:Thrombin; D014357:Trypsin
D002241:Carbohydrates; D046911:Macromolecular Substances; D010446:Peptide Fragments; D005337:Fibrin; D005340:Fibrinogen; D002268:Carboxypeptidases; D002918:Chymotrypsin; D010196:Pancreatic Elastase; D014357:Trypsin; D013917:Thrombin; D005341:Fibrinolysin; D013820:Thermolysin; D003488:Cyanogen Bromide