The steroid alcohol and estrogen sulfotransferases in rodent and human mammary tumors.
Rodent and human mammary tumor systems were investigated to relate the steroid alcohol and estrogen sulfotransferase activities to the hormoanl dependency of the tumor as determined by estrogen receptor content. Unlike the normal mammary gland or the hyperplastic alveolar nodule, rodent mammary neoplasms displayed significant levels of these two sulfotransferases. In the hormone-independent mouse tumors produced from out-growth lines D1, D2, and D8, high dehydroepiandrosterone sulfotransferase activity was characteristic of the rapidity with which hyperplastic alveolar nodules developed into a neoplasms (V-max = 52.8 versus 1.8 fmoles/min/mg protein) while estrone sulfotransferase activity was either not detectable or low (V-max = 5.5 fmoles). After oophorectomy of mice bearing slowly developing tumors, both sulfotransferases in the nonregressing neoplasms showed marked increases in activity (V-max dehydroepiandrosterone = 30.0 fmoles; V-max estrone = 18.5 fmoles). Strain differences not the estrogen receptor content of hormone-dependent rat mammary tumors. In Wistar-Lewis rats the steroid alcohol sulfotransferase activity was at least 35 times higher than in the Sprague-Dawley strain. As was observed in the mouse mammary tumor, Sprague-Dawley rat neoplasms that grew in the absence of ovarian hormones contained significantly greater levels of the steroid alcohol sulfotransferase. Possible correlaion between presence of the steroid alcohol sulfotransferase and the estrogen receptor protein was observed in a limited number of human breast carcinomas.
D000438:Alcohols; D000818:Animals; D001943:Breast Neoplasms / Q000201:enzymology*; D002369:Castration; D002642:Chick Embryo; D003687:Dehydroepiandrosterone; D004967:Estrogens; D004970:Estrone; D005260:Female; D006801:Humans; D007700:Kinetics; D008325:Mammary Neoplasms, Experimental / Q000201:enzymology*; D010053:Ovary; D011230:Precancerous Conditions / Q000201:enzymology; D051381:Rats; D011917:Rats, Inbred Lew; D011956:Receptors, Cell Surface; D013466:Sulfurtransferases / Q000378:metabolism*
D000438:Alcohols; D004967:Estrogens; D011956:Receptors, Cell Surface; D004970:Estrone; D003687:Dehydroepiandrosterone; D013466:Sulfurtransferases