title: Translation of messenger ribonucleic acid from isolated pancreatic islets and human insulinomas.
abstract: RNA preparations from isolated rat pancreatic islets and from human insulinomas were injected into oocytes of Xenopus laevis which were then incubated with [3H]leucine. Acid-ethanol extracts of the oocytes were immunoprecipitated with anti-insulin serum using the double antibody technique. Sodium dodecyl sulfate disc gel electrophoresis of the immunoprecipitates showed the presence of an insulin-displaceable immunoreactive material with a molecular weight of about 18,000 in extracts from oocytes injected with RNA of 9-11 S. This immunoreactive product was not detected in extracts from oocytes injected with buffer or 4-8S RNA or RNA heavier than 11 S. These observations suggest the involvement of a precursor larger than proinsulin in the biosynthesis of insulin.
mesh_terms: D007516:Adenoma, Islet Cell / Q000378:metabolism*; D000818:Animals; D005260:Female; D006801:Humans; D007328:Insulin / Q000096:biosynthesis; D007515:Islets of Langerhans / Q000378:metabolism*; D009865:Oocytes / Q000378:metabolism; D014176:Protein Biosynthesis; D011498:Protein Precursors; D012333:RNA, Messenger / Q000378:metabolism*; D012334:RNA, Neoplasm / Q000378:metabolism*; D051381:Rats; D014981:Xenopus
chemical_list: D007328:Insulin; D011498:Protein Precursors; D012333:RNA, Messenger; D012334:RNA, Neoplasm