Regulation of penicillin acylase in Escherichia coli by cyclic AMP.
1. Cyclic AMP was found to stimulate penicillin acylase activity. 2. It also overcame the repression of glucose and restored enzyme synthesis to the non-repressed levels. 3. The conversion of inactive enzyme precursor into active enzyme was not stimulated by cyclic AMP in cells in which protein synthesis was inhibited by chloramphenicol. 4. Cyclic AMP failed to stimulate enzyme production in cells in which messenger RNA synthesis was arrested by rifampicin or inducer removal. 5. Cyclic AMP appears to participate in the regulation of this enzyme at the transcriptional level.
D000581:Amidohydrolases / Q000378:metabolism*; D000242:Cyclic AMP / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology*; D005947:Glucose / Q000494:pharmacology; D007700:Kinetics; D010399:Penicillin Amidase / Q000378:metabolism*
D000242:Cyclic AMP; D000581:Amidohydrolases; D010399:Penicillin Amidase; D005947:Glucose