title: Kinetics of the reactions between streptokinase, plasmin and alpha 2-antiplasmin.
abstract: Streptokinase reacts very rapidly with human plasmin (rate constant 5.4 S 10(7) M-1 s-1) forming a 1:1 stoichiometric complex which has a dissociation constant of 5 X 10(-11) M. This plasmin-streptokinase complex is 10(5) times less reactive towards alpha 2-antiplasmin than plasmin, the inhibition rate constant being 1.4 X 10(2) M-1 s-1. The loss of reactivity of the streptokinase-plasmin complex towards alpha 2-antiplasmin is independent of the lysine binding sites in plasmin since low-Mr plasmin, which lacks these sites, and plasmin in which the sites have been blocked by 6-aminohexanoic acid, are both equally unreactive towards alpha 2-antiplasmin on reaction with streptokinase. The plasmin-streptokinase complex binds to Sepharose-lysine and Sepharose-fibrin monomer in the same fashion as free plasmin, showing that the lysine binding sites are fully exposed in the complex. Bovine plasmin is rapidly inhibited by human alpha 2-antiplasmin (k1 = 1.6 X 10(6) M-1 s-1) and similarly loses reactivity towards the inhibitor on complex formation with streptokinase (50% binding at 0.4 microM streptokinase).
mesh_terms: D001667:Binding, Competitive; D002846:Chromatography, Affinity; D005337:Fibrin; D005341:Fibrinolysin / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D011485:Protein Binding; D013300:Streptokinase / Q000378:metabolism; D000979:alpha-2-Antiplasmin
chemical_list: D000979:alpha-2-Antiplasmin; D005337:Fibrin; D013300:Streptokinase; D005341:Fibrinolysin