Demonstration of enzymes related to myelinogenesis in established human brain cell cultures.
CNP, CGalT and PAPS-CST, enzymes involved in myelinogenesis were demonstrated in cultures derived from human brain. Little or no CNP activity could be found in cultures derived from non-neurogenic tissue. Transformation of brain cultures by PML-SV40 virus resulted in a 3 to 10-fold increase in the specific activity of CNP.
D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000201:enzymology*; D002417:Cattle; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D002518:Ceramides; D002554:Cerebrosides; D005227:Fatty Acids; D006602:Hexosyltransferases / Q000378:metabolism*; D006801:Humans; D051379:Mice; D009186:Myelin Sheath / Q000201:enzymology*; D010724:Phosphoadenosine Phosphosulfate; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D013539:Simian virus 40; D013466:Sulfurtransferases / Q000378:metabolism*; D014531:Uridine Diphosphate Galactose
D002518:Ceramides; D002554:Cerebrosides; D005227:Fatty Acids; D014531:Uridine Diphosphate Galactose; D010724:Phosphoadenosine Phosphosulfate; D006602:Hexosyltransferases; D013466:Sulfurtransferases; D015087:2',3'-Cyclic-Nucleotide Phosphodiesterases; D010727:Phosphoric Diester Hydrolases