title: The nucleotide sequence of a precursor to the glycine- and threonine-specific transfer ribonucleic acids of Escherichia coli.
abstract: The primary nucleotide sequence of an Escherichia coli tRNA precursor molecule has been determined. This precursor RNA, specified by the transducing phage lambdah80dglyTsuA36 thrT tyrT, accumulates in a mutant strain temperature-sensitive for RNase P activity. The 170-nucleotide precursor RNA is processed by E. coli extracts to form mature tRNA Gly 2 suA36 and tRNA Thr ACU/C. The sequence of the precursor is pG-U-U-C-C-A-G-G-A-U-G-C-G-G-G-C-A-U-C-G-U-A-U-A-A-U-G-G-C-U-A-U-U-A-C-C-U-C-A-G-C-C-U-N-C-U-A-A-G-C-U-G-A-U-G-A-U-G-C-G-G-G-T-psi-C-G-A-U-U-C-C-C-G-C-U-G-C-C-C-G-C-U-C-C-A-A-G-A-U-G-U-G-C-U-G-A-U-A-U-A-G-C-U-C-A-G-D-D-G-G-D-A-G-A-G-C-G-C-A-C-C-C-U-U-G-G-U-mt6A-A-G-G-G-U-G-A-G-m7G-U-C-G-G-C-A-G-T-psi-C-G-A-A-U-C-U-G-C-C-U-A-U-C-A-G-C-A-C-C-A-C-U-UOH(tRNA sequences are italicized). It contains the entire primary nucleotide sequences of tRNA Gly2 suA36 and tRNA Thr ACU/C, including the common 3'-terminal sequence, CCA. Nineteen additional nucleotides are present, with 10 at the 5' end, 3 at the 3' end, and the remaining 6 in the inter-tRNA spacer region. RNase P cleaves the precursor specifically at the 5' ends of the mature tRNA sequences.
mesh_terms: D001345:Autoradiography; D001483:Base Sequence; D003090:Coliphages; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli; D005998:Glycine; D010179:Pancreas / Q000201:enzymology; D010727:Phosphoric Diester Hydrolases; D010761:Phosphorus Radioisotopes; D012343:RNA, Transfer / Q000032:analysis*; D012260:Ribonucleases; D012910:Snake Venoms; D013154:Spleen / Q000201:enzymology; D013912:Threonine
chemical_list: D010761:Phosphorus Radioisotopes; D012910:Snake Venoms; D013912:Threonine; D012343:RNA, Transfer; D012260:Ribonucleases; D010727:Phosphoric Diester Hydrolases; D005998:Glycine