Characterization of the parathyrin receptor in renal plasma membranes by labelled hormone and labelled antibody binding techniques.
The parathyrin receptor in renal cortex has been investigated by studying the binding of 125I-labelled parathyrin, or of unlabelled parathyrin detected with 125I-labelled antibodies, to a partially purified plasma membrane fraction. The kinetics of hormone uptake demonstrated a biphasic response in both systems at 22 degrees C but this phenomenon was not detectable at 37 degrees C. Specific displacement of lactoperoxidase labelled 125I-labelled parathyrin occurred with 8 ng unlabelled bovine parathyrin. The apparent affinity constant was 2.3-10(8) M(-1) and the apparent binding capacity of the membranes 1.25 pmol/mg protein. Using the labelled antibody technique the receptor showed maximal binding at pH 7.0-7.5. As little as 80 pg bovine parathyrin produced a significant increase in binding of labelled anti-bovine parathyrin antibody and saturation of binding sites was demonstrated at 2.5 pmol/mg protein. Oxidized hormone showed undetectable binding. Treatment of membranes with phospholipases A or D, or Trypsin greatly reduced subsequent hormone binding. Prior incubation of membranes with 1-34 synthetic parathyrin decreased the binding of intact hormone whereas gastrin, insulin and glucagon had no effect. Growth hormone and calcitonin slightly increased parathyrin binding.
D000818:Animals; D000937:Antigen-Antibody Reactions; D001666:Binding Sites, Antibody; D002417:Cattle; D002462:Cell Membrane / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D002645:Chickens; D007672:Kidney Cortex / Q000378:metabolism*; D007700:Kinetics; D010281:Parathyroid Hormone / Q000378:metabolism*; D010740:Phospholipases / Q000494:pharmacology; D011485:Protein Binding; D011506:Proteins / Q000276:immunology / Q000378:metabolism; D051381:Rats; D011956:Receptors, Cell Surface / Q000187:drug effects
D010281:Parathyroid Hormone; D011506:Proteins; D011956:Receptors, Cell Surface; D010740:Phospholipases