Inactivation of tumor cell-associated feline oncornavirus for preparation of an infectious virus-free tumor cell immunogen.
Ultraviolet (UV) and thermal methods of inactivating the oncogenic potential of C-type particle-producing feline oncornavirus-induced tumor cells were developed. The techniques were evaluated by several parameters for their use in preparation of cellular immunogens. The UV inactivation dose required to reduce the number of focus-forming units per ml by 1 log10 for FL-74 lymphoblastoid cell-associated feline leukemia virus was 44,000 ergs/sq mm, and the thermal inactivation dose required to reduce the number of focus-forming units per ml by 1 log10 at 45 degrees was 16 min. Inactivation of greater than 6 log10 of virus per ml associated with 4 x 10(8) cells required a UV dose of 270,000 ergs/sq mm, 100 min at 45 degrees or 3 min at 56 degrees. All three treatments concomitantly destroyed the replicating potential of FL-74 cells as shown by their inability to propagate under normal growth conditions and to incorporate [3H]thymidine into nuclear DNA. UV inactivation and thermal inactivation at 45 degrees allowed the best retention of feline oncornavirus-associated cell membrane antigen. A 50% loss in antigenic activity was observed as a result of 56 degrees treatment, but this method was the only one that did not destroy the surface structural integrity of FL-74 cells.
D000914:Antibodies, Viral / Q000032:analysis; D000956:Antigens, Viral / Q000528:radiation effects; D002455:Cell Division / Q000528:radiation effects; D002460:Cell Line; D002470:Cell Survival; D002478:Cells, Cultured / Q000473:pathology; D004307:Dose-Response Relationship, Radiation; D006358:Hot Temperature; D005256:Leukemia Virus, Feline / Q000276:immunology / Q000528:radiation effects; D008223:Lymphoma / Q000276:immunology*; D009374:Neoplasms, Experimental / Q000276:immunology; D014466:Ultraviolet Rays
D000914:Antibodies, Viral; D000956:Antigens, Viral