Radio-enzymic assay of oxo steroids by their reduction with the tritiated form of reduced nicotinamide--adenine dinucleotide.
A method of analysis is proposed based on the enzyme-catalysed transfer of tritium from [3H]NADH to suitable substrates. Its practicability is demonstrated on the examples of oestrone and progesterone with the respective use of the 3 beta, 17 beta- and 3 alpha, 20 beta-hydroxysteroid oxidoreductase. Specificity is tested by application to the analysis of the plasma of pregnant women and measurement of the 3H/14C ratios on purification of the enzymic reduction products.
D004970:Estrone / Q000097:blood; D005260:Female; D006801:Humans; D006913:Hydroxysteroid Dehydrogenases; D007664:Ketosteroids / Q000032:analysis*; D008722:Methods; D009243:NAD; D010084:Oxidation-Reduction; D011247:Pregnancy; D011374:Progesterone / Q000097:blood; D014316:Tritium
D007664:Ketosteroids; D009243:NAD; D014316:Tritium; D004970:Estrone; D011374:Progesterone; D006913:Hydroxysteroid Dehydrogenases