The synthesis of beta-galactosidase by constitutive and other regulatory mutants of Escherichia coli in chemostat culture.
The synthesis of beta-galactosidase by an E. coli constitutive mutant was examined in a chemostat using glucose-, glycerol-, succinate- or N-limited growth media. Except for glucose-grown bacteria, the steady-state intracellular level of beta-galactosidase was maximal at dilution rates between 0-2 and 0-3 h-1. At higher dilution rates enzyme synthesis was reduced by catabolite repression, which could be relieved by the addition of cyclic AMP. With a catabolite-resistant mutant (UV5c), no decrease in enzyme level at high dilution rates were observed. All mutants examined were constitutive and gave decreased enzyme levels at low dilution rates, with the exception of lac-/F'lac UV5c mutants where the enzyme levels rose at low dilution rates. Hyper-producing mutants were isolated but were unstable. A constitutive mutant growing on glycerol-limited media was considered the most suitable for large-scale production of beta-galactosidase in a chemostat.
D001431:Bacteriological Techniques; D003470:Culture Media; D000242:Cyclic AMP / Q000494:pharmacology; D004790:Enzyme Induction; D004794:Enzyme Repression; D004926:Escherichia coli / Q000201:enzymology* / Q000254:growth & development; D005696:Galactosidases / Q000096:biosynthesis*; D005947:Glucose; D005990:Glycerol; D007544:Isopropyl Thiogalactoside / Q000494:pharmacology; D009154:Mutation; D013386:Succinates
D003470:Culture Media; D013386:Succinates; D007544:Isopropyl Thiogalactoside; D000242:Cyclic AMP; D005696:Galactosidases; D005947:Glucose; D005990:Glycerol