Studies on interaction between histone V (f2c) and deoxyribonucleic acids.
Histone V (2fc) from chick erythroctes was used in the study of its interaction with DNA from various sources. Complexes between this histone and DNA were formed using the procedure of continuous NaCl gradient dialysis in urea. Two physical methods, namely thermal denaturation and circular dichroism (CD), were used as analytical tools. Thermal denaturation of nucleohistone V with chick or calf thymus DNA shows three melting bands: band I at 45-50 degrees corresponds to free base pairs; band II at 75-79 degrees, and band III at 90-93 degrees correspond to histone-bound base pairs. In histone-bound regions, there are 1.5 amino acid residues/nucleotide in nucleohistone V. In contrast, a value between 2.9 and 3.3 was determined for nucleohistone I (fl) (H. J. Li (1973), Biopolymers 12, 287). Similar melting properties have been observed for histone V complexed with bacterial DNA from Micrococcus luteus. Histone V binding to DNA induces a slight transition from a B-type CD spectrum to a C-type spectrum. Trypsin treatment of nucleohistone V reduces melting band III much more effectively than band II. Such a treatment also restores DNA to B conformation in the free state. Reduction of the melting bands of nucleohistone V by polylysine binding follows the order of I greater than II greater than III, accompanied by the increase of a new band at 100 degrees. When two bacterial DNAs of varied A + T (adenine + thymine) content simultaneously compete for the binding of histone V, the more (A " T)-rich DNA is selectively favored. Under experimental conditions described here, Clostridium perfringens DNA with 69% A + T is bound by histone V in preference to chicken DNA with 56% A + T although the latter has natural sequences for histone V binding.
D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002645:Chickens; D002843:Chromatin / Q000032:analysis; D002942:Circular Dichroism; D003016:Clostridium perfringens; D004247:DNA; D004269:DNA, Bacterial; D004912:Erythrocytes / Q000032:analysis; D006657:Histones / Q000097:blood; D007700:Kinetics; D008239:Lysine; D008837:Micrococcus; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D010455:Peptides; D011485:Protein Binding; D011487:Protein Conformation; D011489:Protein Denaturation; D013696:Temperature; D013950:Thymus Gland; D014357:Trypsin
D002843:Chromatin; D004269:DNA, Bacterial; D006657:Histones; D010455:Peptides; D004247:DNA; D014357:Trypsin; D008239:Lysine