Backstimulation by blastogenic factor in the primed LD typing test: possible role in anomalous responses.
The anomalous false-positive results often found in the primed lymphocyte typing (PLT) test seem to be partially due to the release of blastogenic factor (BF) from the stimulating cell, resulting in back stimulation of the primed lymphocyte. These anomalous responses can be greatly reduced by treating the stimulator cell with high doses of irradiation or with puromycin.
D002455:Cell Division / Q000187:drug effects / Q000528:radiation effects; D006680:HLA Antigens; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects / Q000528:radiation effects; D007959:Lymphocyte Culture Test, Mixed; D008937:Mitomycins / Q000494:pharmacology; D011691:Puromycin / Q000494:pharmacology
D006680:HLA Antigens; D008937:Mitomycins; D011691:Puromycin