Regulation of purine metabolism. Adenylosuccinate synthetase from Novikoff ascites tumor cells.
Adenylosuccinate synthetase has been partially purified from Novikoff ascites tumor cells. The properties of the protein are quite different from the enzyme from rat liver in that the Km for asparate is higher and the K1 for the feedback inhibitor AMP is also higher. The antibiotic hadacidin has a preferential inhibitory effect on the tumor enzyme. These results suggest that the Novikoff ascites tumor enzyme is less sensitive to normal feedback controls but may be more sensitive to specific antitumor drugs.
D000249:Adenosine Monophosphate / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001224:Aspartic Acid / Q000494:pharmacology; D006528:Carcinoma, Hepatocellular / Q000201:enzymology*; D005246:Feedback; D006160:Guanosine Triphosphate / Q000494:pharmacology; D007292:Inosine Nucleotides / Q000494:pharmacology; D007700:Kinetics; D008025:Ligases / Q000302:isolation & purification / Q000378:metabolism*; D008113:Liver Neoplasms; D009374:Neoplasms, Experimental / Q000201:enzymology; D051381:Rats
D000900:Anti-Bacterial Agents; D007292:Inosine Nucleotides; D001224:Aspartic Acid; D000249:Adenosine Monophosphate; D006160:Guanosine Triphosphate; D008025:Ligases