A plasma inhibitor of the renin-antirenin reaction and the in vitro generation of angiotensin I.
In the course of studies designed to develop a radioimmunoassay system for the detection of renin, we have identified in human plasma a potent inhibitor that interferes with the renin-antirenin reaction. Utilizing gel filtration, this renin-antirenin inhibitory activity was found to have the same molecular size as renin substrate. However, it could be separated from renin substrate by ion-exchange chromatography. When fractions containing this activity were tested in an in vitro system containing renin and renin substrate, they were found to inhibit the generation of angiotensin I.
D000804:Angiotensin II / Q000096:biosynthesis*; D000818:Animals; D001681:Biological Assay; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003168:Complement Fixation Tests; D003864:Depression, Chemical; D004591:Electrophoresis, Polyacrylamide Gel; D010450:Endopeptidases / Q000097:blood; D006801:Humans; D006929:Hyperaldosteronism / Q000097:blood; D006973:Hypertension / Q000097:blood; D010949:Plasma; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay; D012083:Renin / Q000097:blood / Q000276:immunology; D000092502:Renin Inhibitors
D000804:Angiotensin II; D010450:Endopeptidases; D012083:Renin; D000092502:Renin Inhibitors