A novel pathway for phosphatidylcholine catabolism in rat brain homogenates.
Rat brain homogenates incubated with exogenous [32-P] phosphatidylcholine liberated: LYSO[32-P] phosphatidylcholine, sn-glycero-3-[32-P] phosphorylcholine, [32-P] phosphorylcholine, sn-gleycero-3-[32-P] phosphate and 32-Pi. Further investigation showed that [32-P] phosphorylcholine was released exclusively from sn-glycero-3-[32-P] phosphorylcholien by a novel diesterase activity. We propose that the enzyme be termed L-3-glycerylphosphinicocholine cholinephosphohydrolase (EC 3.1.4.-). Parallel experiments on rat liver homogenates and a P815Y mouse mastocytoma cell-lysate, revealed no diesterase activity.
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002460:Cell Line; D005997:Glycerylphosphorylcholine; D008099:Liver / Q000201:enzymology; D008244:Lysophosphatidylcholines; D012515:Mast-Cell Sarcoma / Q000201:enzymology; D051379:Mice; D010713:Phosphatidylcholines / Q000378:metabolism*; D010740:Phospholipases / Q000378:metabolism; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D051381:Rats
D008244:Lysophosphatidylcholines; D010713:Phosphatidylcholines; D005997:Glycerylphosphorylcholine; D010740:Phospholipases; D010727:Phosphoric Diester Hydrolases