"Early" simian-virus-40-specific RNA contains information for tumor antigen formation and chromatin replication.
Simian virus 40 (SV40) induces tumor (T)-antigen formation, chromatin replication, and mitosis in primary mouse kidney cells arrested in G0 phase of the mitotic cycle. The temporal and quantitative relation between these early virus-specific reactions led to the hypothesis that the early SV40 mRNA contains information necessary for T-antigen formation and induction of cellular DNA synthesis. To get direct experimental evidence for this hypothesis, the early strand of SV40 DNA was transcribed in vitro by Escherichia coli DNA-dependent RNA polymerase and the SV40-specific cRNA was transferred by microinjection into epitheloid cells of confluent primary mouse kidney cultures. T-antigen formation and stimulation of DNA synthesis were investigated in the recipient cells. The experimental results obtained agree with the hypothesis that T-antigen is a virus-coded protein and that the early virus-specific mRNA contains information necessary for stimulation of cellular DNA replication in the arrested cells.
D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D002843:Chromatin / Q000378:metabolism*; D004247:DNA / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000378:metabolism*; D012367:RNA, Viral / Q000378:metabolism*; D013539:Simian virus 40 / Q000378:metabolism*; D013997:Time Factors; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000096:biosynthesis
D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D002843:Chromatin; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; D004247:DNA