Non-histone chromosomal proteins from virus-transformed and untransformed 3T3 mouse fibroblasts.
A peak in the non-histone chromosomal protein polyacrylamide gel electrophoresis profiles has been detected which is higher in log phase 3T3 and 3T3/SV40 cells than in density-inhibited 3T3 cells. Radioactive incorporation is substantially higher into this peak in log phase 3T3 than in 3T3/SV40 and density-inhibited 3T3 cells. Reversion of 3T3/SV40 cells with dibutyryl cyclic AMP and theophylline produces increased radioactive incorporation into the peak. Electrophoresis of non-histone chromosomal proteins extracted at different stages of the cell cycle in density inhibited 3T3 cells following serum stimulation shows a cyclic variation in the amount of this peak with maximum accumulation in late G1. In contrast the height of an equivalent peak in synchronously growing 3T3/SV40 cells remains constant throughout the cell cycle. It is postulated that the protein(s) of this peak may have a regulatory role in cell growth.
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002455:Cell Division; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D002875:Chromosomes / Q000378:metabolism*; D003470:Culture Media; D004247:DNA / Q000096:biosynthesis; D004273:DNA, Neoplasm / Q000096:biosynthesis; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000378:metabolism*; D051379:Mice; D009363:Neoplasm Proteins / Q000096:biosynthesis; D009698:Nucleoproteins / Q000096:biosynthesis; D013539:Simian virus 40 / Q000378:metabolism*; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors
D000596:Amino Acids; D003470:Culture Media; D004273:DNA, Neoplasm; D009363:Neoplasm Proteins; D009698:Nucleoproteins; D004247:DNA; D013936:Thymidine