Genetically restricted immune responses in guinea pigs primed in vivo with antigen-bearing macrophages.
Guinea pigs injected intradermally with antigen pulsed macrophages generate a population of immune T cells that proliferate in vitro on second exposure to antigen. T cells from F1 (2 X 13) guinea pigs immunized with DNP-OVA on one parental macrophage respond in vitro only to DNP-OVA on macrophages identical to those used for immunization and not to DNP-OVA associated with the other parental macrophages. These results demonstrate that the immunogenicity of antigen is dependent upon the macrophages used for priming in that, with this approach, strain 2 or 13 guinea pigs immunized with allogeneic macrophages pulsed with antigen do not respond to either allogeneic or syngeneic antigen-bearing macrophages. However, lysates of antigen-pulsed macrophages can still immunize either allogeneic or syngeneic recipient via their own macrophages. F1 (2 X 13) guinea pigs are immunized by insulin B chain pulsed strain 13 macrophages (responder) but not by strain 2 macrophages (nonresponder) suggesting that whether a F1 (nonresponder X responder) guinea pig recognizes antigen bound to a parental macrophage is genetically restricted before immunization to the same extent as the donor parental macrophages used for immunization.
D000818:Animals; D000941:Antigens / Q000235:genetics*; D002450:Cell Communication; D004136:Dinitrobenzenes / Q000276:immunology; D005802:Genes, MHC Class II; D006168:Guinea Pigs; D006824:Hybridization, Genetic; D007114:Immunization; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008264:Macrophages / Q000276:immunology*; D010047:Ovalbumin / Q000276:immunology
D000941:Antigens; D004136:Dinitrobenzenes; D010047:Ovalbumin