Properties of enzymes. V. Catalase inhibition of glucose oxidase reaction.
The radical produced aerobically in the glucose oxidase reaction reduces the oxidated cytochrome c. The extent of reduction depends on the concentrations of substrate (glucose) and enzyme. Superoxide dismutase purified from various sources does not inhibit the cytochrome c reduction, but catalase does, in proportion to its concentration. This inhibition, which might be utilized for quantitative catalase determinations, provides further evidence for the formation of H2O2 in the glucose oxidase reaction.
D000818:Animals; D001230:Aspergillus / Q000201:enzymology; D002374:Catalase / Q000494:pharmacology*; D002417:Cattle; D003574:Cytochrome c Group; D004912:Erythrocytes / Q000201:enzymology; D005949:Glucose Oxidase / Q000037:antagonists & inhibitors*; D006736:Horses; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology; D013482:Superoxide Dismutase / Q000494:pharmacology
D003574:Cytochrome c Group; D005949:Glucose Oxidase; D002374:Catalase; D013482:Superoxide Dismutase