Non-lytic, non-ionic detergent extraction of plasma membrane constituents from normal and transformed fibroblasts.
A technique has been developed for the selective extraction of plasma membrane protein constituents from normal and transformed cell employing non-ionic detergents. The extraction procedure does not damage cells as judged by cell viability, 51Cr release, and trypan blue staining. Lactoperoxidase-catalyzed iodination followed by detergent extraction permits demonstration of a 100,000 dalton protein which is found on the surface of normal but not transformed hamster and mouse fibroblasts.
D002458:Cell Fractionation / Q000379:methods; D002460:Cell Line; D002462:Cell Membrane / Q000648:ultrastructure*; D002470:Cell Survival; D002471:Cell Transformation, Neoplastic; D003902:Detergents; D005347:Fibroblasts / Q000648:ultrastructure; D011092:Polyethylene Glycols; D011136:Polysorbates; D013539:Simian virus 40
D003902:Detergents; D011136:Polysorbates; D011092:Polyethylene Glycols