Rate of virus-specific RNA synthesis in synchronized chicken embryo fibroblasts infected with avian leukosis virus.
The rate of avian leukosis virus (ALV)-specific RNA synthesis has been examined in bot- uninfected and ALV-infected synchronized chicken embryo fibroblasts. RNA from cells labeled for 2h with [3H]uridine was hybridized with avian myeloblastosis virus poly(dC)-DNA, and the hybridized RNA was analyzed with poly(I)-spephadex chromatography. Approximately 0.5% of the RNA synthesized in ALV-infected cells was detected as virus specific, and no more than a twofold variation in the rate of synthesis was detected at different times in the cell cycle. In synchronized uninfected chicken embryo fibroblasts, approximately 0.03% of the RNA synthesized was detected as virus specific, and no significant variation in the rate of synthesis was observed during the cell cycle. Treatment of ALV-infected chicken embryo fibroblasts with cytosine arabinoside or colchicine was used to block cells at different stages in the cell cycle. The rates of virus-specific RNA synthesis in cells so treated did not differ significantly from the rates in either stationary or unsynchronized virus-infected chicken embryo fibroblasts. These findings support the conclusion that after the initial division of an ALV-infected chicken embryo fibroblast and the initiation of virus RNA synthesis, the rate of virus-specific RNA synthesis is independent of the cell cycle.
D000818:Animals; D001354:Avian Leukosis Virus / Q000254:growth & development / Q000378:metabolism*; D009189:Avian Myeloblastosis Virus / Q000032:analysis; D002642:Chick Embryo; D003078:Colchicine / Q000494:pharmacology; D003561:Cytarabine / Q000494:pharmacology; D004279:DNA, Viral; D005347:Fibroblasts / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000096:biosynthesis*
D004279:DNA, Viral; D012367:RNA, Viral; D003561:Cytarabine; D003078:Colchicine