Interaction of cholera enterotoxin with cultured adrenal tumor cells.
In the adrenal tumor cell system ganglioside Gm1 inhibited cholera enterotoxin (CT)-induced steroidogenesis if it was preincubated with the toxin or added to adrenal cells 10 min before CT. In the preincubation studies a molar ratio of Gm1 to toxin of 3:1 was necessary for half-maximal inhibition of steroidogenesis. On the other hand, horse serum anticholeragenoid neutralized the steroidogenic response to cell-bound CT by 50% if it was added to adrenal monolayer cultures 15 min after the toxin. Specific antiserum was able to neutralized 20% of the toxin-induced activity even if it was added to adrenal cultures 2 h after CT. Phase contrast microscopy demonstrated that partial neutralization of the biochemical effect of CT by horse serum anticholeragenoid was accompanied by partial prevention of toxin-induced rounding of adrenal cells. Further studies showed that pretreatment of cultured adrenal cells with a maximal dose of CT increased cyclic adenosine 3'-5'-monophosphate formation in response to a maximal stimulating dose of adrenocorticotropin. This result suggested potentiation of hormonal activation of adenylate cyclase in intact adrenal tumor cells in response to CT.
D000310:Adrenal Gland Neoplasms / Q000378:metabolism*; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000818:Animals; D000992:Antitoxins; D002771:Cholera; D000242:Cyclic AMP / Q000032:analysis; D004357:Drug Synergism; D004768:Enterotoxins / Q000378:metabolism* / Q000494:pharmacology; D005732:Gangliosides / Q000494:pharmacology; D006736:Horses; D066298:In Vitro Techniques; D009374:Neoplasms, Experimental / Q000378:metabolism*; D009500:Neutralization Tests
D000992:Antitoxins; D004768:Enterotoxins; D005732:Gangliosides; D000324:Adrenocorticotropic Hormone; D000242:Cyclic AMP