The control of anaerobic glycolysis by glucose transport and ouabain in slices of hepatoma 3924A.
1. The activities of glycolysis and K-+ transport have been studied in slices of Morris hepatoma 3924A incubated under anaerobic conditions in the presence of different concentrations of glucose (1-50 mM). 2. Ouabain-sensitive net transport of K-+ was observed at all glucose concentrations greater than 1 mM; ouabain reduced the rate of glycolysis by about 25% at all glucose concentrations able to support ion transport. 3. The net entry of glucose into the intracellular phase was studied at varying glucose concentrations. The rate of glucose entry was similar to the rate of glucose utilisation by anaerobic glycolysis at medium concentrations of 10 mM and less, but exceeded the rate of glycolysis at 20 mM and above. 4. The glucose entry was not Na-+-dependent and was not inhibited by ouabain. 5. The results suggest (a) that the reduction in glycolytic activity caused by ouabain is not due to an inhibition of glucose transport and (b) that the glucose transport system of this poorly differentiated hepatoma has properties similar to that of normal liver.
D000693:Anaerobiosis; D000818:Animals; D001693:Biological Transport, Active; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism* / Q000494:pharmacology; D006019:Glycolysis / Q000187:drug effects; D007700:Kinetics; D007773:Lactates / Q000378:metabolism; D008113:Liver Neoplasms; D009374:Neoplasms, Experimental / Q000378:metabolism; D010042:Ouabain / Q000494:pharmacology*; D011188:Potassium / Q000378:metabolism; D051381:Rats; D012964:Sodium / Q000494:pharmacology; D013696:Temperature; D013997:Time Factors
D007773:Lactates; D010042:Ouabain; D012964:Sodium; D005947:Glucose; D011188:Potassium