Activity of human adenylate cyclase from human fat cell membranes.
Purified adipocytes plasma membranes have been prepared from human adipose tissue. The presence of an adenylate cyclase sensitive to epinephrine and fluoride has been demonstrated. Activation of the adenylate cyclase was usually 2 to 4 fold in the presence of epinephrine 5.10-5M and 8 to 10 fold in the presence of fluoride 10 mM. The adenylate cyclase from human adipose tissue was insensitive to glucagon and ACTH; these results are in support of previous studies of lipolysis in isolated fact cells or tissue fragments from human adipose tissue.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000262:Adenylyl Cyclases / Q000378:metabolism*; D000273:Adipose Tissue / Q000378:metabolism; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000328:Adult; D000368:Aged; D000818:Animals; D002462:Cell Membrane; D000242:Cyclic AMP / Q000096:biosynthesis; D003597:Cytosine Nucleotides / Q000494:pharmacology; D004789:Enzyme Activation; D004837:Epinephrine / Q000494:pharmacology; D005459:Fluorides / Q000494:pharmacology; D005934:Glucagon / Q000494:pharmacology; D006160:Guanosine Triphosphate / Q000494:pharmacology; D006801:Humans; D008875:Middle Aged; D051381:Rats
D003597:Cytosine Nucleotides; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D000324:Adrenocorticotropic Hormone; D005934:Glucagon; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D005459:Fluorides; D004837:Epinephrine