title: A comparison of the interfacial interactions of the apoprotein from high density lipoprotein and beta-casein with phospholipids.
abstract: The conformations adopted by beta-casein and the total apoprotein from serum high density lipoprotein when spread at the air-water interface are compared; the monolayer data are consistent with the apoprotein being alpha-helical and the beta-casein being disordered with segments distributed in loops and trains. The penetration of these hydrophobic proteins into phosphatidylcholine monolayers in different physical states was investigated. More protein can penetrate into monolayers when they are in the liquid-expanded state; for penetration at constant total surface area the lateral compressibility of the lipid is an important factor. The charge and conformation of the polar group of the phospholipid does not have a major influence on the interaction. The mixed films of lipid and protein have a mosaic structure; probably the beta-casein is in a compressed state whereas the apoprotein is extended as alpha-helices in the plane of the interface. The chain-length depedences of the interaction of the apoprotein with phosphatidylcholine monolayers and bilayers are different; when the apoprotein binds to bilayers of shorter-chain phosphatidylcholines it alters the shape of the lipid-water interface whereas with monolayers the interface remains planar throughout.
mesh_terms: D001059:Apoproteins; D001665:Binding Sites; D002364:Caseins; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood; D008567:Membranes, Artificial; D008954:Models, Biological; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010718:Phosphatidylserines; D010743:Phospholipids; D011312:Pressure; D011485:Protein Binding; D011487:Protein Conformation; D013499:Surface Properties
chemical_list: D001059:Apoproteins; D002364:Caseins; D008075:Lipoproteins, HDL; D008567:Membranes, Artificial; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010718:Phosphatidylserines; D010743:Phospholipids