[The in vitro and in vivo stability of the entrapment of methotrexate in negatively charged liposomes after sterile filtration (author's transl)].
The preparation of sterile artificial liposomes (phosphatidylcholine:cholesterol:dicethylphosphate=5:5:1) by Millipore filtration is described. Conditions for storing liposome entrapped Methotrexate have been found which allow storage of the drug in the entrapped form over a period of at least 4 months. It is shown that intravenously injected liposome entrapped Methotrexate is retained for at least 15 hours in the particulate form. During this time no free Methotrexate is detectable in the blood.
D002784:Cholesterol; D004355:Drug Stability; D006801:Humans; D008081:Liposomes; D008727:Methotrexate / Q000097:blood; D010713:Phosphatidylcholines; D013997:Time Factors
D008081:Liposomes; D010713:Phosphatidylcholines; D002784:Cholesterol; D008727:Methotrexate
Die in vitro und in vivo Stabilität des Einschlusses von Methotrexat in negativ geladenen Liposomen nach Sterilfiltration.