Yeast phosphoglycerate mutate. Cyanogen bromide cleavage and amino acid sequence of an active-site peptide.
The molecular weight and amino acid composition of phosphoglycerate mutase from yeast were determined. CNBr cleavage produced a large (190-residue) fragment and a small (60-residue) fragment. Tryptic and chymotryptic peptides derived from the large fragment were fractionated by ion-exchange chromatography. Peptides from two histidine-containing regions were isolated and the amino acid sequences were determined. Correlation of these data with X-ray-crystallographic evidence shows that the histidine residue in the sequence Arg-Leu Asn-Glu-Arg-His-Tyr-Gly-Asp-Leu-Glu-Gly-Lys is located at the active site.
D000595:Amino Acid Sequence; D001665:Binding Sites; D003488:Cyanogen Bromide; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis; D010736:Phosphoglycerate Mutase / Q000032:analysis*; D010770:Phosphotransferases / Q000032:analysis*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
D010446:Peptide Fragments; D010770:Phosphotransferases; D010736:Phosphoglycerate Mutase; D003488:Cyanogen Bromide