title: The Pasteur effect and catabolite repression in an oxidative yeast, Kluyveromyces lactis.
abstract: The presence of the Pasteur effect in Kluyveromyces lactis grown in glucose was shown by azide-stimulated glucose fermentation. Extracts from these cells contained ATP-sensitive phosphofructokinase activity. Cells grown on succinate oxidized glucose slowly at first without azide-stimulated rates of fermentation. Phosphofructokinase in these cells was ATP-insensitive. The activity of NAD+-isocitrate dehydrogenase in cell extracts did not require AMP activation. These results suggested the presence of a Pasteur effect in glucose-grown but not in succinate-grown K. lactis, mediated by (a) ATP inhibition of phosphofructokinase (b) possibly via feedback control of glucose transport, but not by AMP activation of isocitrate dehydrogenase. Azide inhibition of the Pasteur effect during growth of the cells did not lead to catabolite repression of respiratory activity. The results therefore suggest that the Pasteur effect does not inhibit the development of a Crabtree effect in oxidative yeasts.
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D001203:Ascomycota / Q000378:metabolism*; D001386:Azides / Q000494:pharmacology; D004794:Enzyme Repression; D005947:Glucose / Q000378:metabolism*; D006019:Glycolysis; D007521:Isocitrate Dehydrogenase / Q000378:metabolism; D010732:Phosphofructokinase-1 / Q000378:metabolism; D004718:Saccharomycetales / Q000378:metabolism*; D013386:Succinates / Q000378:metabolism
chemical_list: D001386:Azides; D013386:Succinates; D000255:Adenosine Triphosphate; D007521:Isocitrate Dehydrogenase; D010732:Phosphofructokinase-1; D005947:Glucose