Correlated effects of cigarette smoke components on alveolar macrophage adenosine triphosphatase activity and phagocytosis.
An initial examination was made of the hypothesis that one action of cigarette smoke components on pulmonary alveolar macrophage function involves the inhibition of contractile protein adenosine triphosphatase activity. Pulmonary alveolar macrophage calcium-dependent adenosine triphosphatase activity, magnesium-dependent adenosine triphosphatase activity, sodium-potassium-dependent adenosine triphosphatase activity, phagocytosis, and cell adhesiveness were measured in the presence of cigarette smoke, acrolein, ouabain, and ethacrynic acid. Calcium-dependent adenosine triphosphatase activity, magnesium-dependent adenosine triphosphatase activity, phagocytosis, and adhesiveness were inhibited by smoke and ethacrynic acid, but not by ouabain. Acrolein, a component of smoke, inhibited phagocytosis, adhesiveness, and calcium-dependent adenosine triphosphatase activity, indicating that another component of smoke must be effective at inhibiting magnesium-dependent adenosine triphosphatase activity. Sodium-potassium-dependent adenosine triphosphatase activity was inhibited by ouabain and ethacrynic acid, but not by smoke or acrolein. Finally, sulfhydryl reagents at least partially protected the macrophages against the inhibitory actions of each of the agents. The results are in accord with recently obtained experimental evidence that calcium-dependent adenosine triphosphatase and, perhaps, magnesium-dependent adenosine triphosphatase play a role in phagocytosis. The data also suggest that smoke components affect a number of macrophage activities, including adhesion and phagocytosis, by altering the cell's contractile apparatus.
D000171:Acrolein / Q000494:pharmacology; D000251:Adenosine Triphosphatases / Q000502:physiology*; D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D004976:Ethacrynic Acid / Q000494:pharmacology; D008264:Macrophages / Q000201:enzymology / Q000502:physiology*; D010042:Ouabain / Q000494:pharmacology; D010587:Phagocytosis / Q000187:drug effects*; D010947:Plants, Toxic; D011650:Pulmonary Alveoli / Q000201:enzymology / Q000502:physiology*; D011817:Rabbits; D012906:Smoke; D014026:Nicotiana
D012906:Smoke; D010042:Ouabain; D000171:Acrolein; D000251:Adenosine Triphosphatases; D004976:Ethacrynic Acid