A quantitative assay for concanavalin A- and Ricinus communis agglutinin-mediated agglutinations of rat ascites hepatoma cells. Relationship between concanavalin A binding and cell agglutination.
A simple quantitative assay method was developed for the agglutination of rat ascites hepatoma cells mediated by Concanavalin A or Ricinus communis agglutinin. This method was based on the principle that the turbidity of a cell suspension is proportional to the sum of the cross-sectional area of cells and aggregatesmas predicted by the theoretical consideration, the turbidity decreased when cells were aggregated and the decrease was a function of the average number of the cells in aggregates. The agglutinability of the cells, judged by this method, showed a maximum value at a certain concentration of the agglutinin. By further addition of the agglutinin, the agglutinability slightly decreased from the maximum. These phenomena were observed both for Concanavalin A and Ricinus communis agglutinin. The binding and the agglutination experiments using [3-H]concanavalin A revealed that the binding to approx;0% of the total receptors caused a maximal agglutination. This suggested that the receptors responsible for the agglutination constitute only a small part of the total receptors on the surface.
D000372:Agglutination Tests; D000818:Animals; D001666:Binding Sites, Antibody; D006528:Carcinoma, Hepatocellular / Q000276:immunology* / Q000378:metabolism; D003208:Concanavalin A / Q000032:analysis / Q000378:metabolism; D007700:Kinetics; D037102:Lectins / Q000032:analysis; D008113:Liver Neoplasms; D008433:Mathematics; D008722:Methods; D009374:Neoplasms, Experimental / Q000276:immunology / Q000378:metabolism; D037121:Plant Lectins; D010947:Plants, Toxic; D011485:Protein Binding; D051381:Rats; D012278:Ricinus; D013997:Time Factors
D037102:Lectins; D037121:Plant Lectins; D003208:Concanavalin A