A simple and inexpensive method for the preparation of (beta,gamma-32P) guanosine 5'-triphosphate.
A rapid and inexpensive method has been developed for the synthesis of 32P-labeled guanosine 5'-triphosphate (GTP). When yolk platelets isolated from brine shrimp cysts are incubated with 32PPi at pH 5.8 and in the presence of 10 mM MgCl2 and 5 mM dithiothreitol, the primary compound formed is [beta,gamma-32P]GTP. The synthetic reaction is catalyzed by the yolk platelet enzyme, GTP : GTP guanylyltransferase, which has been demonstrated to be important in the biosynthesis of diguanosine 5'-tetraphosphate (Gp4G), the major purine nucleotide in brine shrimp yolk platelets and encysted embryos.
D000818:Animals; D033364:Decapoda / Q000196:embryology / Q000378:metabolism*; D011756:Diphosphates / Q000378:metabolism; D004229:Dithiothreitol / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000378:metabolism; D004983:Ethanolamines / Q000378:metabolism; D006150:Guanine Nucleotides / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000096:biosynthesis* / Q000302:isolation & purification; D007553:Isotope Labeling; D009713:Nucleotidyltransferases / Q000378:metabolism; D010761:Phosphorus Radioisotopes
D011756:Diphosphates; D004983:Ethanolamines; D006150:Guanine Nucleotides; D010761:Phosphorus Radioisotopes; D006160:Guanosine Triphosphate; D009713:Nucleotidyltransferases; D004229:Dithiothreitol