Difference in the action of ethyl and methyl methane sulfonates on DNA template activity for RNA synthesis in vitro.
RNA produced in vitro from alkylated T7 DNA has been characterized by polyacrylamide gel electrophoresis. Methylation of T7 DNA by methyl methane sulfonate reduces RNA chain length. In contrast, ethylation of T7 DNA by ethyl methane sulfonate, while reducing RNA synthesis to the same extent, does not alter chain length.
D004279:DNA, Viral / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D007700:Kinetics; D008698:Mesylates / Q000494:pharmacology*; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D008970:Molecular Weight; D012329:RNA, Bacterial / Q000096:biosynthesis*; D012367:RNA, Viral / Q000096:biosynthesis*; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism; D013698:Templates, Genetic; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects*
D004279:DNA, Viral; D008698:Mesylates; D012329:RNA, Bacterial; D012367:RNA, Viral; D005020:Ethyl Methanesulfonate; D008741:Methyl Methanesulfonate