A quantitative autoradiographic study of nucleolus-associated RNA and DNA synthesis during the eclipse phase in Rous sarcoma virus-infected chicken fibroblasts.
Functional and morphologic differences between the sensitivity of nucleoli of Rous sarcoma virus-transformed cells and that of newly infected cells to the action of actinomycin D (AD) have been demonstrated by quantitative light and electron microscope autoradiography and utilized to investigate the function of the nucleolus in the early stages of infection. After a pulse exposure to low doses of AD, increased RNA synthesis is induced within 80 minutes in the fibrillar portion of the nucleolus by infection. A concomitant increase in the retention of tritiated AD in the nucleolus and a quantitative redistribution of intranuclear and cytoplasmic DNA label are interpreted as evidence for a virus-induced amplification of the binding sites of AD in nucleolar chromatin.
D000818:Animals; D001345:Autoradiography; D001358:Avian Sarcoma Viruses; D001665:Binding Sites; D002466:Cell Nucleolus / Q000187:drug effects / Q000378:metabolism*; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D002642:Chick Embryo; D004273:DNA, Neoplasm / Q000096:biosynthesis*; D003609:Dactinomycin / Q000378:metabolism / Q000494:pharmacology; D005347:Fibroblasts / Q000648:ultrastructure*; D012334:RNA, Neoplasm / Q000096:biosynthesis*; D013997:Time Factors
D004273:DNA, Neoplasm; D012334:RNA, Neoplasm; D003609:Dactinomycin