title: Physical map of the BK virus genome.
abstract: Two new human papovavirus isolates (JMV and MMV) from the urines of patients with Wiskott-Aldrich syndrome were morphologically and serologically identical to BK virus (BKV). The genomes of these two new isolates were found to be indistinguishable from prototype BKV DNA in a variety of nucleic acid hybridization experiments. Like BKV DNA, JMV and MMV DNAs share approximately 20% of their polynucleotide sequences with simian virus 40 DNA. The genome of JMV was indistinguishable from that of BKV by restriction endonuclease analysis; MMV DNA contained three instead of four R-Hind cleavage sites and one rather than no R-HpaII cleavage sites. Physical maps of the BKV and MMV genomes were constructed using restriction endonucleases, and these maps were oriented to the map of simian virus 40 DNA.
mesh_terms: D001739:BK Virus / Q000032:analysis; D001483:Base Sequence; D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004270:DNA, Circular / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis / Q000378:metabolism; D005796:Genes; D006801:Humans; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D011119:Polynucleotides / Q000032:analysis; D011120:Polyomavirus; D013539:Simian virus 40 / Q000032:analysis; D014556:Urine / Q000382:microbiology; D014923:Wiskott-Aldrich Syndrome / Q000382:microbiology
chemical_list: D004270:DNA, Circular; D004279:DNA, Viral; D011119:Polynucleotides; D004262:DNA Restriction Enzymes