The ultraviolet endonuclease of bacteriophage T4. Further characterization.
The T4 ultraviolet endonuclease was previously shown to produce strand incisions (nicks) in ultraviolet-irradiated DNA on the 5' side of thymine dimers. The present studies demonstrate that the purified endonuclease creates 3'-OH and 5'-P termini at the sites of nicking. Photoreactivation of ultraviolet-sensitive sites, thereby demonstrating directly endonucleause has a molecular weight of approximately 18,000 and attacks ultraviolet-irradiated single-stranded Escherichia coli and M-13 DNA.
D000818:Animals; D002417:Cattle; D003090:Coliphages / Q000201:enzymology*; D004267:DNA Viruses / Q000201:enzymology; D004277:DNA, Single-Stranded / Q000528:radiation effects*; D004279:DNA, Viral / Q000528:radiation effects*; D003851:Deoxyribonucleases / Q000378:metabolism*; D004720:Endonucleases / Q000378:metabolism*; D007700:Kinetics; D008970:Molecular Weight; D009691:Nucleic Acid Denaturation; D010727:Phosphoric Diester Hydrolases; D011830:Radiation Effects; D013154:Spleen / Q000201:enzymology; D013941:Thymine / Q000378:metabolism; D013997:Time Factors; D014466:Ultraviolet Rays
D004277:DNA, Single-Stranded; D004279:DNA, Viral; D003851:Deoxyribonucleases; D004720:Endonucleases; D010727:Phosphoric Diester Hydrolases; D013941:Thymine