title: Modulation of stimulated acid secretion by dibutyryl cyclic AMP in the dog stomach.
abstract: An in vivo canine gastric chamber preparation was used to determine effects of exogenous cyclic AMP and dibutyryl cyclic AMP on histamine-driven acid secretion and the role of blood flow in these responses. Histamine was infused intravenously at a submaximal rate 1.2 mu/kg-min) followed by the intraarterial infusion of either cyclic AMP (0.15 mg/kg-min) or dibutyryl cyclic AMP (0.15 mg/kg-min. Acid secretion and mucosal blood flow (aminopyrine clearance) were measured at 15-minute intervals, and electrical potential difference was measured continuously. Histamine alone decreased the potential difference from 82 mV to 48 mV within 60 minutes, increased aminopyrine clearance from 1.0 ml/min to 2.4 ml/min and increased acid output to 220 muEq/15 min. All values returned towards control following termination of histamine infusion. Dibutyryl cyclic AMP significantly increased histamine stimulated acid secretion to 350 muEq/15 min, but did not alter mucosal blood flow or electrical potential. Cyclic AMP did not change secretory, circulatory or electrical response to histamine. Therefore, dibutyryl cyclic AMP appears to increase histamine-stimulated acid secretion, and the effect is not mediated by increased mucosal blood flow.
mesh_terms: D000632:Aminopyrine / Q000378:metabolism; D000818:Animals; D003994:Bucladesine / Q000008:administration & dosage / Q000494:pharmacology*; D000242:Cyclic AMP / Q000008:administration & dosage / Q000494:pharmacology; D004285:Dogs; D004594:Electrophysiology; D005260:Female; D005750:Gastric Juice / Q000378:metabolism*; D005753:Gastric Mucosa / Q000098:blood supply / Q000187:drug effects; D006632:Histamine / Q000008:administration & dosage / Q000494:pharmacology; D007263:Infusions, Parenteral; D008297:Male; D008657:Metabolic Clearance Rate; D012039:Regional Blood Flow / Q000187:drug effects; D013268:Stimulation, Chemical
chemical_list: D000632:Aminopyrine; D003994:Bucladesine; D006632:Histamine; D000242:Cyclic AMP