Electron microscope studies of temperature-sensitive mutants of herpes simplex virus type 2.
Nine temperature-sensitive mutants of herpes simplex virus type 2 representing eight complementation groups were assigned to two classes as a consequence of the virion forms and virus-specific cellular alterations observed in thin sections of mutant-infected human embryonic lung cells grown at the nonpermissive temperature. Mutants in class A, one DNA- and one DNA +, failed to synthesize detectable virus particles. Mutants in class B, 4DNA- and 3DNA+, produced moderate to large numbers of empty nucleocapsids. Dense-cored nucleocapsids were not observed in thin sections of cells infected with any of the nine mutants at this temperature. Virus-specific cellular alterations consisted primarily of margination of chromating and nulcear membrane thickening and duplication.
D002213:Capsid / Q000096:biosynthesis; D002460:Cell Line; D004279:DNA, Viral / Q000096:biosynthesis; D009024:Morphogenesis; D009154:Mutation; D018139:Simplexvirus / Q000254:growth & development / Q000378:metabolism / Q000648:ultrastructure*; D013696:Temperature; D014779:Virus Replication
D004279:DNA, Viral