Leukemia virus infection of mammalian cells: effect on two "transformation-associated" surface properties.
We demonstrated that the productive infection of three different mammalian cell lines with two separate leukemia viruses is sufficient to induce a change in surface architecture that may be detected as enhanced agglutinability with two different plant lectins. Subsequent transformation of one of these cell lines with a chemical carcinogen did not further modify the agglutinability of the cell lines. Using a polyoma virus-transformed derivative of one of the parental lines, we have demonstrated that the LETS protein (whose absence from the surface membrane has been considered a marker of the transformed phenotype) may be present in cells displaying the capacity to plate in soft agar.
D000362:Agar; D000371:Agglutination; D000818:Animals; D002273:Carcinogens; D002455:Cell Division; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured / Q000502:physiology*; D003208:Concanavalin A / Q000494:pharmacology; D006023:Glycoproteins / Q000378:metabolism; D007457:Iodine Radioisotopes; D007784:Lactoperoxidase; D037102:Lectins; D009052:Leukemia Virus, Murine / Q000254:growth & development*; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000378:metabolism; D009600:Nitroquinolines / Q000494:pharmacology; D012377:Rodentia; D013499:Surface Properties
D002273:Carcinogens; D006023:Glycoproteins; D007457:Iodine Radioisotopes; D037102:Lectins; D009363:Neoplasm Proteins; D009600:Nitroquinolines; D003208:Concanavalin A; D000362:Agar; D007784:Lactoperoxidase