Fibroblast surface antigen produced but not retained by virus-transformed human cells.
Normal human fibroblasts contain a cell type-specific glycoprotein antigen (SF) that is known to be slowly shed into the medium and to be present also in human serum.  Immunofluorescence with anti-SF antibodies showed that SF antigen has a highly nonrandom fibrillar distribution in surface of normal fibroblasts.  Simian virus 40-transformed fibroblasts also produced the SF antigen, as shown by radioimmunoassay or immunodiffusion tests, but it was not retained by the surface of these cells.  This creates a major difference between the surfaces of normal and malignant cells.
D000941:Antigens; D001666:Binding Sites, Antibody; D002462:Cell Membrane / Q000276:immunology*; D003588:Cytopathogenic Effect, Viral; D005347:Fibroblasts / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D005779:Immunodiffusion; D007457:Iodine Radioisotopes; D011863:Radioimmunoassay; D013539:Simian virus 40
D000941:Antigens; D007457:Iodine Radioisotopes