Carbohydrate content and regulation following injection of different glycogenolytic enzymes.
The effect of injection of glycogenolytic enzymes on tissue glycogen, blood glucose and plasma insulin was studied in mice. No effects were observed following phosphorylase, whereas the hydrolytic enzymes, alpha-amylase and acid amyloglucosidase depressed liver glycogen. In addition acid amyloglucosidase induced a decrease in blood glucose, a slight elevation of plasma insulin and a marked increase in tolbutamide-stimulated insulin release. At the doses given none of the enzymes affected muscle glycogen. Amyloglucosidase pretreatment markedly enhanced insulin release induced by glibenclamide, leucine, isoleucine, lysine and glucose whereas insulin release stimulated by IPNA, ACTH, glucagon and "CCK-PZ" was unaffected. Injection of acid amyloglucosidase has a profound influence on carbohydrate content and regulation in mice. It is suggested that the dependence or independence of amyloglucosidase activity among the insulin secretagogues tested might reflect different or partially different mechanisms in the process of insulin secretion.
D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000596:Amino Acids / Q000494:pharmacology; D000681:Amylases / Q000494:pharmacology*; D000818:Animals; D001234:Aspergillus niger / Q000201:enzymology; D001786:Blood Glucose / Q000378:metabolism*; D002766:Cholecystokinin / Q000494:pharmacology; D005959:Glucosidases / Q000494:pharmacology*; D005905:Glyburide / Q000494:pharmacology; D006003:Glycogen / Q000378:metabolism*; D007328:Insulin / Q000097:blood*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D009132:Muscles / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D009638:Norepinephrine / Q000031:analogs & derivatives / Q000494:pharmacology; D010179:Pancreas / Q000201:enzymology; D006005:Phosphorylases / Q000494:pharmacology*; D051381:Rats; D013552:Swine; D013997:Time Factors
D000596:Amino Acids; D001786:Blood Glucose; D007328:Insulin; D000324:Adrenocorticotropic Hormone; D006003:Glycogen; D002766:Cholecystokinin; D006005:Phosphorylases; D000681:Amylases; D005959:Glucosidases; D005905:Glyburide; D009638:Norepinephrine