title: A pitfall in the interpretation of data on ligand-protein interaction.
abstract: Binding reactions are usually investigated by separation of free and bound labelled ligand. Underestimation of the amount bound leads to inaccurate estimation of the dissociation constant and to wrong conclusions on the validity of the model of binding. The activation of cyclic AMP-dependent protein kinase is treated as an example.
mesh_terms: D001665:Binding Sites; D000242:Cyclic AMP / Q000378:metabolism; D007700:Kinetics; D008024:Ligands; D008956:Models, Chemical; D011485:Protein Binding; D011494:Protein Kinases / Q000378:metabolism
chemical_list: D008024:Ligands; D000242:Cyclic AMP; D011494:Protein Kinases