The purine nucleotide cycle. Control of phosphofructokinase and glycolytic oscillations in muscle extracts.
Linked oscillations of the glycolytic pathway and the purine nucleotide cycle were studied in particle-free extracts of rat skeletal muscle. Under the conditions used, an accumulation of about 1 muM fructose diphosphate can trigger a sudden increase in phosphofructokinase activity. The activation by fructose diphosphate depends on the presence of AMP. When the AMP concentration drops, phosphofructokinase becomes inhibited, even though the fructose disphosphate concentration remains high. It is concluded that the oscillatory behavior can be of advantage for maintaining a high average [ATP]/[ADP] ratio.
D000244:Adenosine Diphosphate / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D004099:Dihydroxyacetone Phosphate / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D006019:Glycolysis / Q000187:drug effects; D006150:Guanine Nucleotides / Q000378:metabolism; D007773:Lactates / Q000378:metabolism; D008297:Male; D009132:Muscles / Q000187:drug effects / Q000378:metabolism*; D009243:NAD / Q000494:pharmacology; D010732:Phosphofructokinase-1 / Q000378:metabolism*; D011685:Purine Nucleotides / Q000378:metabolism*; D011773:Pyruvates / Q000378:metabolism; D051381:Rats
D006150:Guanine Nucleotides; D007773:Lactates; D011685:Purine Nucleotides; D011773:Pyruvates; D009243:NAD; D000249:Adenosine Monophosphate; D004099:Dihydroxyacetone Phosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D010732:Phosphofructokinase-1