The dexamethasone receptor in the Novikoff Hepatoma. Characterization and changes in concentration and nuclear uptake during glucocorticoid therapy.
Novikoff hepatoma ascites cells, grown intraperitoneally in rats, are shown to possess a high-affinity, low-capacity dexamethasone-binding protein. The receptor protein has an intracellular localization and concentration, association constant (Ka), glucocorticoid specificity and nuclear uptake in the presence of dexamethasone comparable with those of the G-protein of rat liver. During therapy of the tumour-bearing animal with cortisol, marked cyclic variations were observed in the concentration and nuclear uptake of the putative G-protein in the tumor cells; more transient variations were also observed in the Ka value of the receptor protein.
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D003907:Dexamethasone / Q000378:metabolism; D005260:Female; D006854:Hydrocortisone / Q000627:therapeutic use; D007700:Kinetics; D008113:Liver Neoplasms; D009374:Neoplasms, Experimental / Q000378:metabolism; D051381:Rats; D011956:Receptors, Cell Surface; D013256:Steroids / Q000494:pharmacology
D011956:Receptors, Cell Surface; D013256:Steroids; D003907:Dexamethasone; D006854:Hydrocortisone