title: Oxidation of reduced nicotinamide adenine dinucleotide by particles from Mycobacterium lepraemurium.
abstract: Particles from Mycobacterium lepraemurium catalysed the oxidation of NADH with oxygen as the terminal electron acceptor. The preparations contained cytochromes of the a + a3'b and c types, as well as CO-binding pigments. The NADH oxidase activity was sensitive to inhibitors of the flavoprotein system as well as to HQNO and antimycin A. In addition, a cytochrome oxidase sensitive to cyanide was also present. The system was inhibited by the thiol-binding agent, PCMB, and thus indicated the involvement of sulphydryl group in the enzymatic oxidation of NADH. The sensitivity of the NADH oxidase system to all the inhibitors of the respiratory chain and the effect of these inhibitors on the absorption spectra suggested that cytochromes of the b, c, a + a3 types are involved in the transfer of electrons in NADH oxidation.
mesh_terms: D000654:Amobarbital / Q000494:pharmacology; D000968:Antimycin A / Q000494:pharmacology; D001386:Azides / Q000494:pharmacology; D002248:Carbon Monoxide / Q000378:metabolism; D002474:Cell-Free System; D002729:Chloromercuribenzoates / Q000494:pharmacology; D003486:Cyanides; D003580:Cytochromes / Q000378:metabolism; D004140:Dinitrophenols / Q000494:pharmacology; D007700:Kinetics; D009167:Mycobacterium lepraemurium / Q000201:enzymology* / Q000648:ultrastructure; D009243:NAD / Q000378:metabolism*; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D010101:Oxygen Consumption; D010416:Pentachlorophenol / Q000494:pharmacology; D010860:Pigments, Biological / Q000378:metabolism; D011796:Quinacrine / Q000494:pharmacology; D011804:Quinolines / Q000494:pharmacology
chemical_list: D001386:Azides; D002729:Chloromercuribenzoates; D003486:Cyanides; D003580:Cytochromes; D004140:Dinitrophenols; D010860:Pigments, Biological; D011804:Quinolines; D009243:NAD; D000968:Antimycin A; D002248:Carbon Monoxide; D010416:Pentachlorophenol; D009247:NADH, NADPH Oxidoreductases; D000654:Amobarbital; D011796:Quinacrine