Enzymatic and non-enzymatic assay of superoxide dismutase.
Superoxide dismutase from breef brain and rat liver was assayed in an enzymatic system, using xanthine oxidase, and a non-enzymatic system, based on aerobic reduction of nitro-blue tetrazolium in presence of phenazine methosulphate. The non-enzymatic assay is rapid and simple and permits simulatneous analysis of many samples.  Similar results are  found by the two methods of assay of superoxide dismutase.
D000818:Animals; D001921:Brain / Q000201:enzymology; D002417:Cattle; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D008297:Male; D008773:Methylphenazonium Methosulfate; D009243:NAD; D009580:Nitroblue Tetrazolium; D010084:Oxidation-Reduction; D051381:Rats; D013482:Superoxide Dismutase / Q000032:analysis*; D014969:Xanthine Oxidase
D009243:NAD; D009580:Nitroblue Tetrazolium; D008773:Methylphenazonium Methosulfate; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase