Estrogen-receptor binding: relationship to estrogen-induced responses.
The relationship between nuclear binding of the receptor-estrogen complex (R-E) and estrogenic responses was examined. Nuclear-bound R-E was measured by the [3H] estradiol exchange assay, which permits the quantification of R-E as a function of either endogenous or non labeled estrogen. The quantity of nuclear R-E fluctuated as a function of blood levels of estrogen during the estrous cycle in the rat. This indicates that the accumulation of the R-E complex by the nucleus of uterine cells may be of physiologic significance and under the control of endogenous estrogen.
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology; D004965:Estrogen Antagonists; D004967:Estrogens / Q000378:metabolism / Q000494:pharmacology*; D005260:Female; D066298:In Vitro Techniques; D009256:Nafoxidine / Q000494:pharmacology; D010902:Pituitary Gland / Q000648:ultrastructure; D051381:Rats; D011956:Receptors, Cell Surface / Q000187:drug effects*; D013268:Stimulation, Chemical; D013997:Time Factors; D014599:Uterus / Q000254:growth & development / Q000648:ultrastructure
D004965:Estrogen Antagonists; D004967:Estrogens; D011956:Receptors, Cell Surface; D009256:Nafoxidine; D004958:Estradiol