Characterization of the androgen receptor from a Syrian hamster ductus deferens tumor cell line (DDT1).
The hamster ductus deferens cloned tumor cell line (DDT1) contains a complex steroid receptor protein that binds 3H-labeled 5 alpha-dihydrotestosterone,. Diethylaminoethyl (DEAE) chromatography of cytosol from these cells yields two major receptor peaks of activity. Identification of this steroid binding protein as a cytoplasmic receptor was confirmed by salt dissociation on sucrose gradients, stability of the hormone-receptor complex at 0 degrees C, and the retention patterns on phosphocellulose and DEAE cellulose. Multiple forms of the receptor exist in a single homogeneous cell type. The data support the theory that steroid hormones bind to a cytoplasmic protein receptor composed of dissimilar subunits as the initial step in steroid hormone action.
D002352:Carrier Proteins; D002458:Cell Fractionation; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D003534:Cyproterone / Q000378:metabolism; D013196:Dihydrotestosterone / Q000378:metabolism*; D004958:Estradiol / Q000378:metabolism; D007890:Leiomyosarcoma / Q000378:metabolism; D008297:Male; D008970:Molecular Weight; D009374:Neoplasms, Experimental / Q000378:metabolism; D011374:Progesterone / Q000378:metabolism; D011487:Protein Conformation; D011956:Receptors, Cell Surface; D014649:Vas Deferens / Q000378:metabolism*
D002352:Carrier Proteins; D011956:Receptors, Cell Surface; D013196:Dihydrotestosterone; D011374:Progesterone; D004958:Estradiol; D003534:Cyproterone