A cholinoceptor antiserum: its pharmacological properties.
1 Sera of rabbits immunized against a nocotinic receptor-rich fraction, obtained from the electric organ of Torpedo marmorata, were tested for their pharmacological activity on different in vitro preparations. 2 Sera containing antibodies against the nicotinic receptor blocked neuromuscular transmission in the phrenic-nerve hemidiaphragm preparation without affecting the muscle responses evoked by direct electrical stimulation. Complement inactivated sera were still active. Immune sera, incubated for 15 min with a receptor-rich fraction, lost their activity. 3 The immune sera antagonized the responses elicited by acetylcholine on the frog rectus abdominis. 4 The immune sera tested in vitro decreased the compound action potential evoked in the superior cervical ganglion of the rat by electrical stimulation of the preganglionic nerve. 5 The sera did not show any activity on muscarinic receptors of the guinea-pig ileum preparation. 6 It is concluded that in sera obtained from immunized rabbits a substance is present with curare-like action, and that this activity is probably due to the presence of antibodies against the nicotinic receptor.
D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D003964:Diaphragm; D005399:Fishes / Q000276:immunology; D005727:Ganglia, Spinal / Q000187:drug effects; D006168:Guinea Pigs; D007082:Ileum / Q000187:drug effects; D007106:Immune Sera / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects; D009469:Neuromuscular Junction / Q000276:immunology; D010791:Phrenic Nerve / Q000187:drug effects; D011817:Rabbits / Q000276:immunology; D011894:Rana pipiens; D051381:Rats; D011950:Receptors, Cholinergic / Q000187:drug effects*; D009435:Synaptic Transmission / Q000187:drug effects
D007106:Immune Sera; D011950:Receptors, Cholinergic