title: Fish gonadotropin(s). I. Bioassay of salmon gonadotropin(s) in vitro with immature trout gonads.
abstract: 1. The effect of salmon gonadotropin(s) (SG) on cyclic AMP (cAMP) levels in immature trout gonadal tissue of both sexes was measured by radioimmunoassay. 2. A dose-response line was obtained to SG in gonads of both male and female trout. 3. As little as 0.45 SG units (1 SG unit = 1 mug NIH-LH-S18 in the chick bioassay) significantly increased cAMP formation in the presence of 8 mM theophylline; mammalian LH, FSH, LTH, ACTH, TSH and HCG were inactive. 4. The assay for SG was investigated with respect to time of incubation and two phosphodiesterase inhibitors; some conditions for the cAMP radioimmunoassay (cAMP-RIA) were compared.
mesh_terms: D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006065:Gonadotropins, Pituitary / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D010053:Ovary / Q000187:drug effects / Q000378:metabolism*; D010902:Pituitary Gland / Q000032:analysis; D012474:Salmon; D013737:Testis / Q000187:drug effects / Q000378:metabolism*; D013806:Theophylline / Q000494:pharmacology; D014337:Trout
chemical_list: D006065:Gonadotropins, Pituitary; D013806:Theophylline; D000242:Cyclic AMP