Early events in polyoma virus infection: attachment, penetration, and nuclear entry.
The plaque-assay technique was used as a tool to determine the optimal conditions for adsorption of polyoma virions to host cells. Using these optimal conditions of adsorption, an electron microscopy study of the early events of infection was performed. By electron microscopy and autoradiography, it was demonstrated that both the viral coat proteins and DNA arrive simultaneously in the nucleus as early as 15 min postinfection. When horseradish peroxidase-labeled virions, pseudovirions, and capsids were used to infect cells, only the particles with nucleic acid or a factor(s) associated with the nucleic acid, i.e., histones, appeared to enter the nucleus. Moreover, when virions were used to infect either permissive or nonpermissive cells, identical early events of viral infection, i.e., adsorption, penetration, and nuclear transport, were observed, suggesting that these early events of infection are a property of the virion and not the host cell.
D000327:Adsorption; D001345:Autoradiography; D002460:Cell Line; D002462:Cell Membrane / Q000382:microbiology; D002467:Cell Nucleus / Q000382:microbiology; D046508:Culture Techniques; D004279:DNA, Viral / Q000378:metabolism; D006735:Horseradish Peroxidase; D008854:Microscopy, Electron; D008954:Models, Biological; D011120:Polyomavirus / Q000254:growth & development* / Q000378:metabolism; D014764:Viral Proteins / Q000378:metabolism; D014779:Virus Replication
D004279:DNA, Viral; D014764:Viral Proteins; D006735:Horseradish Peroxidase