Radiation enhanced reactivation of herpes simplex virus: effect of caffeine.
Ultaviolet enhanced (Weigle) reactivation of UV-irradiated herpes simplex virus in UV-irradiated CV-1 monkey kidney cell monolayers was decreased by caffeine. X-ray enhanced reactivation of UV-irradiated virus in X-irradiated monolayers (X-ray reactivation) and UV- or X-ray-inactivated capacity of the cells to support unirradiated virus plaque formation were unaffected by caffeine. The results suggest that a caffeine-sensitive process is necessary for the expression of Weigle reactivation for herpes virus. Since cafeine did not significantly affect X-ray reactivation, different mechanisms may be responsible for the expression of Weigle reactivation and X-ray reactivation.
D002110:Caffeine / Q000494:pharmacology*; D002460:Cell Line; D004260:DNA Repair; D004279:DNA, Viral / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D011831:Radiation Genetics; D018139:Simplexvirus / Q000528:radiation effects*; D014466:Ultraviolet Rays; D010948:Viral Plaque Assay; D014965:X-Rays
D004279:DNA, Viral; D002110:Caffeine