[In vitro evaluation of antiviral inhibitors by means of a turbidity method (author's transl)].
The paper presents a photometric method for the determination of virus-induced alterations (cytopathogenic effects) of cells based on nephelometric analysis. These were taken at varying time intervals after infection and with different multiplicity of infection. It was demonstrated that suppression of virus-induced CPE by the known inhibitors could also be followed by turbidity measurements. The in vitro experiments were carried out in the 2 following virus-cell-systems: Pseudorabies virus in chick-embryo cells and Mengo virus in FL-cells, respectively. According to these studies the measurements were extended to other virus strains and inhibitors (antibiotics, chemicals, and antimetabolites). The results obtained confirm the applicability of the photometric method for the screening of antiviral drugs.
D000547:Amantadine / Q000494:pharmacology; D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D002642:Chick Embryo; D003513:Cycloheximide / Q000494:pharmacology; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects*; D006564:Herpesviridae / Q000472:pathogenicity*; D011558:Herpesvirus 1, Suid / Q000187:drug effects / Q000472:pathogenicity*; D066298:In Vitro Techniques; D009980:Influenza A virus / Q000187:drug effects / Q000472:pathogenicity; D008574:Mengovirus / Q000187:drug effects / Q000472:pathogenicity*; D009391:Nephelometry and Turbidimetry; D009522:Newcastle disease virus / Q000472:pathogenicity; D012845:Sindbis Virus / Q000187:drug effects / Q000472:pathogenicity; D013997:Time Factors
D000998:Antiviral Agents; D003513:Cycloheximide; D000547:Amantadine
Eine nephelometrische Methode für die in vitro-Testung antiviraler Substanzen