Effects of indomethacin on intestinal secretion, prostaglandin E and cyclic AMP: evidence against a role for prostaglandins in cholera toxin-induced secretion.
The effects of indomethacin on intestine mucosal cAMP, intestinal fluid secretion, and mucosal and fluid PGE were studied in rabbits in vivo following challenge with cholera toxin. Indomethacin had no effect on cholera toxin-induced fluid secretion or cAMP accumulation. Inhibition of PGE synthesis was achieved by the administration of two but not one injection of indomethacin. These studies provide evidence against a role for PGE in mediating cholera toxin-induced secretion and point out the need to measure prostaglandin levels when using prostaglandin synthetase inhibitors in vivo.
D000818:Animals; D002771:Cholera; D000242:Cyclic AMP / Q000378:metabolism*; D004347:Drug Interactions; D005260:Female; D007213:Indomethacin / Q000008:administration & dosage / Q000494:pharmacology*; D007273:Injections, Intramuscular; D007413:Intestinal Mucosa / Q000378:metabolism; D007419:Intestinal Secretions / Q000187:drug effects*; D007583:Jejunum / Q000378:metabolism; D008297:Male; D011458:Prostaglandins E / Q000378:metabolism*; D011817:Rabbits; D012636:Secretory Rate / Q000187:drug effects; D013997:Time Factors; D014118:Toxins, Biological / Q000494:pharmacology*
D011458:Prostaglandins E; D014118:Toxins, Biological; D000242:Cyclic AMP; D007213:Indomethacin