Selective in vitro growth of T lymphocytes from normal human bone marrows.
Selective growth of T lymphocytes occurred when unfractionated normal human bone marrow cells were cultured with conditioned medium obtained from phytohemagglutinin-stimulated normal human lymphocytes (Ly-CM). Cultures of up to 90 percent T cells have been maintained for more than 9 months. The T cells exhibited a strict growth dependence upon Ly-CM and were consistently negative for Epstein-Barr viral information.
D001854:Bone Marrow Cells; D002469:Cell Separation; D002478:Cells, Cultured; D003470:Culture Media; D004854:Herpesvirus 4, Human / Q000032:analysis; D006801:Humans; D037102:Lectins; D008938:Mitosis; D013601:T-Lymphocytes / Q000276:immunology / Q000382:microbiology
D003470:Culture Media; D037102:Lectins