Genetic analysis of succinate utilization in enzyme I mutants of the phosphoenolpyruvate: sugar phosphotransferase system in Escherichia coli.
Studies on the reversion characteristics of Escherichia coli strains carrying various mutations in the pts region have led to the recognition of a mutation, suc-1, with a previously undescribed phenotype. Strains carrying the suc-1 mutation grow normally on most sources of carbon but are unable to utilize succinate effectively. The suc-1 mutation can be separated genetically from the tightly linked ptsI6 mutation. Reversion of suc-1 mutants for growth on succinate yields interesting classes of suppressor mutations.
D002241:Carbohydrates; D002874:Chromosome Mapping; D000242:Cyclic AMP / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D005632:Fructose / Q000378:metabolism; D008040:Genetic Linkage; D008353:Mannitol / Q000378:metabolism; D008358:Mannose / Q000378:metabolism; D009097:Multienzyme Complexes / Q000096:biosynthesis*; D009154:Mutation; D010641:Phenotype; D010728:Phosphoenolpyruvate; D010770:Phosphotransferases / Q000096:biosynthesis*; D013386:Succinates / Q000378:metabolism*; D013489:Suppression, Genetic; D014161:Transduction, Genetic
D002241:Carbohydrates; D009097:Multienzyme Complexes; D013386:Succinates; D005632:Fructose; D008353:Mannitol; D010728:Phosphoenolpyruvate; D000242:Cyclic AMP; D010770:Phosphotransferases; D008358:Mannose