Functional evaluation of lung mitochondria.
Activity of lung mitochondria was studied with a preparation of isolated mitochondria. This was correlated with responses of the isolated perfused lung as determined from surface fluorescence measurements and tissue redox couples. Lung mitochondria demonstrated substrate oxidation, respiratory control, and Caplus plus activation of alpha-glycerophosphate dehydrogenase. In response to oxidative inhibitors, the isolated lung showed increased surface fluorescence and increased tissue ratios of redox couples indicating reduction of pyridine nucleotides. The results demonstrate functional activity of lung mitochondria in situ.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D010856:Columbidae; D005453:Fluorescence; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D005994:Glycerophosphates / Q000378:metabolism; D007773:Lactates / Q000378:metabolism; D008168:Lung / Q000378:metabolism / Q000648:ultrastructure*; D008928:Mitochondria / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D011817:Rabbits; D051381:Rats; D012756:Sheep
D005994:Glycerophosphates; D007773:Lactates; D009243:NAD; D005993:Glycerolphosphate Dehydrogenase; D002118:Calcium