title: TEM-induced gene mutations at enzyme loci in the mouse.
abstract: Strain DBA/2J male mice were treated with triethylenemelamine (TEM) and subsequently mated to strain C57BL/6J females. Tissues from F1 progeny produced in these crosses were then examined using starch gel electrophoresis for the presence of presumed induced mutations at a series of 11 specific enzyme loci. In the course of this study, four heritable mutations were identified at the following loci: Es-1, Ldh-1, Pgm-1, and Gpi-1. Of these four, the first two were apparently segregating in parental males and were not TEM-induced. Both of these are viable and fertile in the heterozygous and homozygous condition, and neither confers any readily apparent deleterious effect to the animal. The latter mutations (Pgm-1 and Gpi-1) are presumably induced. Although viable and fertile in the heterozygous state, we have not recovered any offsping homozygous for either of these two mutations.
mesh_terms: D000818:Animals; D003433:Crosses, Genetic; D004798:Enzymes / Q000235:genetics*; D004950:Esterases / Q000235:genetics; D005260:Female; D005796:Genes; D005956:Glucose-6-Phosphate Isomerase / Q000235:genetics; D007770:L-Lactate Dehydrogenase / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL / Q000235:genetics; D008811:Mice, Inbred DBA / Q000235:genetics; D009153:Mutagens; D009154:Mutation; D010733:Phosphoglucomutase / Q000235:genetics; D014265:Triethylenemelamine / Q000494:pharmacology*
chemical_list: D004798:Enzymes; D009153:Mutagens; D007770:L-Lactate Dehydrogenase; D004950:Esterases; D005956:Glucose-6-Phosphate Isomerase; D010733:Phosphoglucomutase; D014265:Triethylenemelamine