Rapid interaction of prolactin with mouse mammary gland explants.
Exposure of mouse mammary gland explants to prolactin at 0 degrees C, for periods as brief as 10 seconds, caused a stimulation of labeled uridine incorporation into RNA during a subsequent incubation for 4 h at 37 degrees C. Furthermore, a 2-h wash of the prolactin-exposed explants in media at 0 degrees C did not attenuate the hormonal effect. A similar exposure of explants to insulin, followed by a 2-h wash at 0 degrees C, caused the abolition of the insulin stimulation of labeled uridine incorporation into RNA. The results suggest that there is a rapid and relatively stable interaction of prolactin with the mammary gland, while the interaction of insulin with this tissue would appear to be less stable.
D000818:Animals; D005260:Female; D007328:Insulin / Q000494:pharmacology; D008321:Mammary Glands, Animal / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D011388:Prolactin / Q000378:metabolism / Q000494:pharmacology*; D012313:RNA / Q000096:biosynthesis; D011956:Receptors, Cell Surface / Q000187:drug effects; D014529:Uridine / Q000378:metabolism
D007328:Insulin; D011956:Receptors, Cell Surface; D012313:RNA; D011388:Prolactin; D014529:Uridine