The 3' terminal oligonucleotide of E. coli 16S ribosomal RNA: the sequence in both wild-type and RNase iii- cells is complementary to the polypurine tracts common to mRNA initiator regions.
Application of Sanger techniques to the analysis of the 3' terminal oligonucleotide from E. coli 32-P-labelled 16 S rRNA yields the sequence AUCACCUCCUUAOH. This sequence is identical in RNA isolated from two wild-type strains (MRE600 and E. coli B, SY106) and from a mutant strain (AB301/105) defective in RNase III. Data presented here explains the previous derivation of an incorrect sequence (AUCCUCACUUCAOH) by others. The functional significance of complementarity between the 3' terminus of 16S rRNA and poly-purine tracts commonly found in mRNA initiator regions is discussed.
D001483:Base Sequence; D002234:Carbodiimides; D004926:Escherichia coli; D009841:Oligonucleotides / Q000032:analysis*; D010727:Phosphoric Diester Hydrolases; D012329:RNA, Bacterial / Q000032:analysis*; D012335:RNA, Ribosomal / Q000032:analysis*; D012260:Ribonucleases
D002234:Carbodiimides; D009841:Oligonucleotides; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D012260:Ribonucleases; D010727:Phosphoric Diester Hydrolases