The effect of castration and testosterone replacement on specific proteins and androgen levels of the rat epididymis.
The normal weight increase of the epididymis during sexual maturation and its maintenance through adulthood were found to be dependent on the provision of androgens. Binding of [3H]dihydrotestosterone (DHT) to the epididymal 8S cytoplasmic receptor gradually decreased after castration to become undetectable after 25 days. Binding to the androgen binding protein (ABP) was absent 4 days after castration and was not reinduced by 3 weeks of testosterone (T) administration. Unilateral castration for periods of up to 27 days showed the disappearance of ABP with preservation of the 8S receptor on the castrated side, indicating a testicular source for ABP and the epididymal origin of the 8S receptor. The tissue concentrations of T and DHT in the epididymis became undetectable 30 days after castration and were restored to normal values by administration of testosterone in large doses (1.5 mg/100 g BW). Similar results were obtained in rats castrated at 10 days of age and injected with testosterone until 60 days old. The ratio DHT/T was depressed in the castrate and increased with testosterone treatment. The protein content of the epididymis (mg of protein/g wet weight) was also found to be influenced by androgens. Our results show evidence of some mechanisms involved in the trophic effect of androgens upon the epididymis and suggest the possible androgenic control of epididymal 5alpha-reductase activity. They also indicate that a testicular factor is required for the maintenance of the 8S cytoplasmic androgen receptor. It is not known whether this factor is testosterone or some other testicular secretion.
D000728:Androgens / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D002369:Castration; D003593:Cytoplasm / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D013196:Dihydrotestosterone / Q000378:metabolism; D004822:Epididymis / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D009929:Organ Size; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*; D051381:Rats; D011956:Receptors, Cell Surface; D013739:Testosterone / Q000494:pharmacology*; D013997:Time Factors
D000728:Androgens; D011506:Proteins; D011956:Receptors, Cell Surface; D013196:Dihydrotestosterone; D013739:Testosterone