title: Halothane action on lymphocytes does not involve cyclic AMP.
abstract: Both theophylline and halothane inhibited transformation of human lymphocytes by phytohemagglutinin (PHA). Theophylline did not augment the inhibitory action of halothane and depressed PHA transformation of halothane-treated cells to the same extent as that of air-treated cells. Halothane- and air-treated lymphocytes, prior to PHA addition, had the same content of cyclic AMP. The addition of PHA to these cultures raised cyclic-AMP concentrations to the same extent in halothane- and air-treated lymphocytes. Halothane action on PHA-stimulated lymphocytes appears not to involve changes in cyclic nucleotide metabolism.
mesh_terms: D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism*; D006221:Halothane / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D037102:Lectins / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D013806:Theophylline / Q000008:administration & dosage / Q000494:pharmacology
chemical_list: D037102:Lectins; D013806:Theophylline; D000242:Cyclic AMP; D006221:Halothane