Biosynthesis of mouse interferon by translation of its messenger RNA in a cell-free system.
A fraction of mouse RNA containing messenger RNA coding for mouse interferon was translated with high efficiency in a wheat germ system into a fully active product. This product fulfills the criteria for mouse interferon, namely: (1) it was active against vesicular stomatitis virus and herpes simplex virus in mouse cells; (2) its antiviral activity was species specific; (3) its activity was completely neutralized by mouse anti-interferon serum. The synthesis of interferon in this cell-free system requires the presence of spermine.
D000937:Antigen-Antibody Reactions; D002460:Cell Line; D007372:Interferons / Q000096:biosynthesis* / Q000276:immunology / Q000494:pharmacology; D007700:Kinetics; D010944:Plants / Q000378:metabolism*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D018139:Simplexvirus / Q000187:drug effects; D013045:Species Specificity; D014908:Triticum / Q000378:metabolism; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects
D012333:RNA, Messenger; D007372:Interferons