Drug-protein interaction: 8-methoxy psoralen as photosensitizer of enzymes and amino acids.
For a better insight of the molecular basis of the photobiological effects of furocoumarins, the relevance of proteins oxydation by singlet oxygen produced by these substrates under irradiation with long u. v. light was studied. Complex oligomeric as well as simple monomeric purified enzymes with high or low molecular weight and different properties and simple amino acids were irradiated under oxygen in presence of 8-methoxy-psoralen. The effects on both proteins and amino acids were compared with those obtained under similar conditions with typical photosensitizers as methylene blue and Rose Bengal. The results indicated that the photooxydation of proteins, although possible, appears to play a minor role, if any, in the mechanism of action of furocoumarin.
D000596:Amino Acids; D000818:Animals; D002417:Cattle; D005399:Fishes; D005969:Glutamate Dehydrogenase / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D008730:Methoxsalen / Q000494:pharmacology; D009113:Muramidase / Q000378:metabolism*; D009132:Muscles / Q000201:enzymology; D010734:Phosphogluconate Dehydrogenase / Q000378:metabolism*; D010751:Phosphopyruvate Hydratase / Q000378:metabolism*; D010782:Photolysis; D011817:Rabbits; D012260:Ribonucleases / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D013820:Thermolysin / Q000378:metabolism*
D000596:Amino Acids; D010734:Phosphogluconate Dehydrogenase; D005969:Glutamate Dehydrogenase; D012260:Ribonucleases; D009113:Muramidase; D013820:Thermolysin; D010751:Phosphopyruvate Hydratase; D008730:Methoxsalen