[Corticotrope and melanotrope cells in cat, fox, rat and human fetus adenohypophysis: studies with induced fluorescence, cytoimmunologic technics and lead hematoxyline].
Formaldehyde-induced fluorescence, acid-catalyzed or not, methods, and immunocytology with anti-ACTH (1-24), anti-ACTH(17-39), ANTI-BETA-LPH immunserums were applied on the same preparations of cat, fox, rat and human foetus pituitaries. The superpositions of results showed that the pars intermedia and pars distalis corticomelano-lipotrophic cells of fox and cat pituitary, those of human foetal pituitary, and the purely corticotrophics cells of the rat pars distalis contained a fluorogenic probably granular compound. Moreover, the granules of the same cell types were electively revealed on our lymphilized material by plombic hematoxylin. Only the anti-beta-LPH and/or anti-beta-MSH fixing celpls exhibited hypercyanophilic, PAS-positive and bleu alcian-positive caracteristics.
D000311:Adrenal Glands / Q000502:physiology; D000324:Adrenocorticotropic Hormone / Q000032:analysis*; D000818:Animals; D002415:Cats; D005333:Fetus; D005453:Fluorescence; D005455:Fluorescent Antibody Technique; D005557:Formaldehyde; D005589:Foxes; D006416:Hematoxylin; D006651:Histocytochemistry; D006801:Humans; D009074:Melanocyte-Stimulating Hormones / Q000032:analysis*; D010902:Pituitary Gland / Q000166:cytology*; D010903:Pituitary Gland, Anterior / Q000032:analysis / Q000166:cytology*; D051381:Rats; D008083:beta-Lipotropin / Q000032:analysis*
D005557:Formaldehyde; D000324:Adrenocorticotropic Hormone; D009074:Melanocyte-Stimulating Hormones; D008083:beta-Lipotropin; D006416:Hematoxylin
Les cellules corticotropes et mélanotropes de l'adénohypophyse chez le chat, le renard, le rat et le foetus humain : études avec diverses techniques de fluorescence induite, de cyto-immunologie et à l'hématoxyline plombique