[Quantitative cytochemical detection of enzymes in cultured cells following infection with Rous virus].
The method of visual quantitative estimation was employed to determine the index of enzymes activity in embryonal cells cultures infected with Rous virus. In early terms (1-7 days after infection) a moderate production of diformazane was noted in the presence of glucoso-6-phosphatiosomerase, phosphoglucomutase, pyruvatoxidase, NAD. H2- and NADP. H-2-diaphorase, other enzymes showing no distinquishable deviations in the activity, as compared with the normal culture. In later terms (13-27 days) during the proliferation of the transformed cells there was found an increased level of the glycolysis enzymes activity, pentose cycle, hydrolysis of ortho-phosphoric acid monoethers and reduced lemon acid cycle and tissue respiration. Cytoplasmatic vacuoles formed in the transformed cells seem to represent hypertrophic lysosomes and phagolysosomes.
D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001358:Avian Sarcoma Viruses; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D002642:Chick Embryo; D005956:Glucose-6-Phosphate Isomerase / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D006593:Hexokinase / Q000378:metabolism; D006867:Hydrolases / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008247:Lysosomes; D009252:NADPH Dehydrogenase / Q000378:metabolism; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism*; D010733:Phosphoglucomutase / Q000378:metabolism; D010734:Phosphogluconate Dehydrogenase / Q000378:metabolism; D011770:Pyruvate Kinase / Q000378:metabolism; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013997:Time Factors; D014166:Transferases / Q000378:metabolism*; D014617:Vacuoles
D010088:Oxidoreductases; D007770:L-Lactate Dehydrogenase; D010734:Phosphogluconate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D013385:Succinate Dehydrogenase; D009251:NADPH-Ferrihemoprotein Reductase; D009252:NADPH Dehydrogenase; D014166:Transferases; D006593:Hexokinase; D011770:Pyruvate Kinase; D006867:Hydrolases; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D005956:Glucose-6-Phosphate Isomerase; D010733:Phosphoglucomutase
Kolichestvennoe tsitokhimicheskoe vyiavlenia fermentov v kul'turakh kletok posle zarazheniia viruson Raus