Studies on morphological transformation of BALB/3T3-derived clones by murine leukemia virus.
We have described a cell line, UC1-B, derived spontaneously from BALB/3T3 mouse embryo cells, which, unlike the standard BALB/3T3, are morphologically transformed and produce bizarre viral forms in response to murine leukemia virus. Although UC1-B and BALB/3T3 are morphologically similar, and both form contact-inhibited monolayers at confluence, the UC1-B cells are partially transformed because: they grow to a slightly higher saturation density than 3T3 cells, they grow in medium lacking serum growth factors, and they produce tumors in mice. Another clone, 12A-3, derived from BALB/3T3, also transforms and produces bizarre viral forms after infection with murine leukemia virus. Unlike UC1-B cells, the 12A3-8 cells are identical in growth properties to BALB/3T3; therefore, a partially altered morphology is not required for the induction of transformation by murine leukemia virus.
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000382:microbiology; D002471:Cell Transformation, Neoplastic; D002999:Clone Cells; D051379:Mice; D008807:Mice, Inbred BALB C; D008979:Moloney murine leukemia virus / Q000254:growth & development* / Q000648:ultrastructure; D013539:Simian virus 40 / Q000254:growth & development; D014617:Vacuoles / Q000382:microbiology; D014779:Virus Replication