Protamine and polyarginine bacteriolysis. Similarities in its mechanism with chromatin DNA picnosis.
Protamine and polyarginine had bacteriolytic effects indicating their primary sites of action as being wall components and showing bacterial diversity genetically determined. Shake-incubation was required in producing cell-lysis. Studies on Bacillus subtilis revealed a high polycation multiplicity per cell in lytic event displaying multihit lysing kinetics; bacteriolysis was inhibited by trypsin, pronase, purified polyanionic wall polysaccharide, and by dissociative actions of salt hypermolarities used in isolation of nucleic acids. The inactivation of polycation lytic abilities during bacteriolysis was accompanied by modifications in electrophoretic running of protamine and polyarginine. It is suggested as mechanism of cell-lysis, the multiple zonal surface condensations of polyanionic wall components by basic polypeptides, likely similar with chromatin DNA picnosis. This analogy is discussed.
D000900:Anti-Bacterial Agents; D001412:Bacillus subtilis / Q000187:drug effects; D001419:Bacteria / Q000187:drug effects* / Q000378:metabolism; D002843:Chromatin / Q000187:drug effects* / Q000378:metabolism; D004269:DNA, Bacterial / Q000378:metabolism*; D010455:Peptides / Q000494:pharmacology*; D011479:Protamines / Q000494:pharmacology*; D013045:Species Specificity
D000900:Anti-Bacterial Agents; D002843:Chromatin; D004269:DNA, Bacterial; D010455:Peptides; D011479:Protamines; C015462:polyarginine