Intracellular synthesis of mouse mammary tumor virus polypeptides: indication of a precursor glycoprotein.
Mouse mammary tumor virus polypeptides were detected in the cytoplasm of mouse mammary tumor cell cultures using immunological precipitation techniques. The anti-mouse mammary tumor virus serum precipitated the major virion glycoproteins gp49 and gp37.5/33.5 and a viral-related nonvirion glycoprotein of 76,000 daltons. Subcellular fractionation studies revelaed that the cell-associated virion glycoproteins were present in the membrane fraction. Pulsechase experiments indicated that a viral-related nonvirion glycoprotein of 76,000 daltons may be a precursor to one or more of the virion glycoproteins.
D000230:Adenocarcinoma; D000818:Animals; D002458:Cell Fractionation; D002462:Cell Membrane / Q000378:metabolism; D002474:Cell-Free System; D046508:Culture Techniques; D003593:Cytoplasm / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005944:Glucosamine / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism*; D008325:Mammary Neoplasms, Experimental; D008324:Mammary Tumor Virus, Mouse / Q000378:metabolism*; D008715:Methionine / Q000378:metabolism; D051379:Mice; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000378:metabolism*; D010452:Peptide Biosynthesis; D011233:Precipitin Tests; D011498:Protein Precursors / Q000378:metabolism*; D013462:Sulfur Radioisotopes; D014316:Tritium; D014764:Viral Proteins / Q000096:biosynthesis*
D006023:Glycoproteins; D009363:Neoplasm Proteins; D011498:Protein Precursors; D013462:Sulfur Radioisotopes; D014764:Viral Proteins; D014316:Tritium; D008715:Methionine; D005944:Glucosamine