title: Estrogen-induced uterine responses and growth: relationship to receptor estrogen binding by uterine nuclei.
abstract: The relationship between estrogen receptor(R) binding by uterine nuclei and uterotrophic responses was examined. Immature rats received a single injection of estradiol (E2) or estriol (E3) and the following parameters were measured: accumulation and retention of the estrogen receptor by the nuceus of uterine cells; incorporation of 14C-glucose into CO2 lipid, protein and RNA; RNA polymerase activity; water imbibition and increased dry weight. E2 and E3 were of equal potency with regard to the rapid accumulation of R by the nucleus but differed with respect to long term retention of R.  The concentrations of nuclear RE2 and RE3 complexes were equivalent between 1 and 3 hr after estrogen injection; however, by 6 hr RE2 remained significantly elevated while RE3 levels had fallen to control values. E2 and E3 were also of equal potency with respect to the stimulation of enhanced glucose utilization, water imbibition, the incorporation of 14C-glucose into lipid, protein and RNA 3 hours following an injection of the hormone. Likewise the activity of RNA polymerase was equally stimulated by E2 and E3 3 hr after injection. Thus all early uterotropic responses (0-3 hrs) that were measured were equally stimulated by E2 and E3. However, E3 failed to stimulate true uterine growth (increase dry weight 24 hr after injection), whereas E2 produced a significant stimulation of true uterine growth. These data suggest that the RE complex is capable of stimulating early uterotrohic events regardless of which estrogen is present; however, in order to produce true uterine growth the RE complex must be retained in the nucleus for long periods of time. This proposal was tested by the administration of repetitive injections of E3. This treatment resulted in an increase in dry weight that was equivalent to the growth that was produced by repetitive injections of E2. These results demonstrate that E2 and E3 elicit early uterotrophic responses with equal facility following a single injection but that only E2 causes true uterine growth. The ability of  E2 to stimulate true uterine growth appears to be related to the time of residence of the RE complex in the nucleus.
mesh_terms: D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism; D002250:Carbon Radioisotopes; D002467:Cell Nucleus / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology; D004964:Estriol / Q000008:administration & dosage / Q000494:pharmacology; D005260:Female; D005947:Glucose / Q000378:metabolism; D007279:Injections, Subcutaneous; D050356:Lipid Metabolism; D009929:Organ Size; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D051381:Rats; D011956:Receptors, Cell Surface; D013329:Structure-Activity Relationship; D013997:Time Factors; D014316:Tritium; D014599:Uterus / Q000187:drug effects / Q000378:metabolism*
chemical_list: D002250:Carbon Radioisotopes; D011506:Proteins; D011956:Receptors, Cell Surface; D014316:Tritium; D002245:Carbon Dioxide; D004958:Estradiol; D012313:RNA; D012321:DNA-Directed RNA Polymerases; D004964:Estriol; D005947:Glucose