Inhibition of protein synthesis in chloroplasts from plant cells by virginiamycin.
The light-driven incorporation of amino acids by isolated spinach chloroplasts is inhibited by the M component (VM) and not by the S component (VS) of virginiamycin. This inhibitory effect is partially reversible. In chloroplast extracts, poly(U)-directed polyphenylalanine formation is strongly inhibited by VM and not by VS. The in vivo synergistic effect of VM and VS observed in bacteria and algae, does not occur in isolated chloroplasts and chloroplast extracts.
D002736:Chloroplasts / Q000187:drug effects / Q000378:metabolism*; D003624:Darkness; D008027:Light; D010940:Plant Proteins / Q000096:biosynthesis*; D010944:Plants / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism; D014769:Virginiamycin / Q000494:pharmacology*
D010940:Plant Proteins; D014769:Virginiamycin