Development and properties of fructose 1,6-bisphosphatase in the endosperm of castor-bean seedlings.
1. The activity of fructose 1,6-bisphosphatase (EC 3.1.3.11) in the fatty endosperm of castor bean (Ricinus communis) increases 25-fold during germination and then declines. The developmental pattern follows that of catalase, a marker enzyme for gluconeogenesis in this tissue. 2. The enzyme at its peak of development was partially purified, and its properties were studied. It has an optimal activity at neutral pH (7.0-8.0). The apparent Km value for fructose 1,6-bisphosphate is 3.8 X 10(-5) M. The activity is inhibited by AMP allosterically with an apparent Ki value of 2.2 X 10(-4) M. The enzyme hydrolyses fructose 1,6-bisphosphate and not ribulose 1,5-bisphosphate or sedoehptulose 1,7-bisphosphate. 3. Treatment of the partially purified enzyme with acid leads to an 80% decrease in activity. The remaining activity is insensitive to AMP and has optimal activity at pH 6.7 and a high apparent Km value (2.5 X 10(-4) M) for fructose 1.6-bisphosphate. Enzyme extracted from the tissue with water instead of buffer has a similar modification. The effect of acid explains the discrepancies between this report and previous ones on the properties of the enzyme in this tissue. 4. The storage tissues of various fatty seedlings all contain a 'neutral' fructose 1,6-bisphosphatase. The activities of the enzyme from some of the tissues are inhibited by AMP. 5. The properties of the enzyme in fatty seedlings and in green leaves are discussed in comparison with that in animal tissues.
D000249:Adenosine Monophosphate / Q000494:pharmacology; D010367:Arachis / Q000032:analysis; D002367:Ricinus communis / Q000201:enzymology*; D006597:Fructose-Bisphosphatase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D005636:Fructosephosphates / Q000378:metabolism; D006368:Helianthus / Q000201:enzymology; D010944:Plants / Q000201:enzymology; D010947:Plants, Toxic; D012274:Ribulosephosphates / Q000378:metabolism; D012278:Ricinus / Q000201:enzymology*; D013403:Sugar Phosphates / Q000378:metabolism; D003313:Zea mays / Q000201:enzymology
D005636:Fructosephosphates; D012274:Ribulosephosphates; D013403:Sugar Phosphates; D000249:Adenosine Monophosphate; D006597:Fructose-Bisphosphatase