Breakage of human cell DNA after exposure to 3-methylcholanthrene-11,12-oxide.
Damage to and repair of DNA isolated from human neonatal and fetal skin cells were measured by alkaline sucrose gradient analysis. 3-Methylcholanthrene did not induce single-strand breaks in DNA of the cells in culture, whereas the 11,12-oxide of 3-methylcholanthrene was very effective in this regard. The cis-1,2-dihydroxy, trans-11,12-dihydroxy, and cis-11,12-dihydroxy derivatives of 3-methylcholanthrene exerted little effect. The breaks in DNA caused by 3-methylcholanthrene oxide occurred during a 60-min incubation period and were repaired during the following 60 min. Methylmethane sulfonate also induced breaks in the DNA within 60 min.
D002478:Cells, Cultured; D004247:DNA / Q000032:analysis*; D004260:DNA Repair; D006801:Humans; D007231:Infant, Newborn; D008698:Mesylates; D008748:Methylcholanthrene / Q000031:analogs & derivatives* / Q000494:pharmacology; D013997:Time Factors
D008698:Mesylates; D008748:Methylcholanthrene; D004247:DNA