Some properties of vacuoles isolated from Neurospora crassa slime variant.
A method for the isolation of vacuoles based on polybase induced lysis of protoplasts of the cell wall deficient Neurospora crassa slime variant is described. Isolated vacuoles are characterized by 12 to 50 times increased specific activities of several hydrolases as compared with the total homogenate of protoplasts. Total alpha-amino nitrogen, arginine, and polyphosphate are also greatly enriched in these vacuoles. Vacuoles are equipped with a permease for the transport of basic amino acids across the tonoplast.
D000596:Amino Acids / Q000032:analysis; D001120:Arginine / Q000378:metabolism; D006867:Hydrolases / Q000378:metabolism; D009491:Neurospora / Q000502:physiology*; D009492:Neurospora crassa / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure; D010088:Oxidoreductases / Q000378:metabolism; D010710:Phosphates / Q000032:analysis; D011523:Protoplasts / Q000032:analysis / Q000378:metabolism; D014617:Vacuoles / Q000032:analysis / Q000378:metabolism / Q000502:physiology
D000596:Amino Acids; D010710:Phosphates; D001120:Arginine; D010088:Oxidoreductases; D006867:Hydrolases