Transformation of mouse cells after infection with ultraviolet irradiation-inactivated herpes simplex virus type 2.
A transformed mouse cell line (H238) was obtained following the infection of 238 mouse cells with ultraviolet (UV) irradiation-inactivated herpes simplex virus type 2 (HSV-2). The transformed cells produced tumors with a 100% incidence within 8 weeks in 6-week-old syngeneic BALB/c mice at an inoculum of 1 times 10(6). Indirect immunofluorescence (IF) tests revealed the presence of HSV antigens in the transformed cells. Antibodies to HSV-2 were found in the sera of tumor-bearing animals by neutralization and IF techniques. Neither HSV-2 infectious virus nor viral antigens could be detected by the transfer of transformed-cell DNA into permissive cells.
D000818:Animals; D000914:Antibodies, Viral; D000918:Antibody Specificity; D000956:Antigens, Viral; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D004273:DNA, Neoplasm / Q000302:isolation & purification; D004279:DNA, Viral / Q000302:isolation & purification; D066298:In Vitro Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000276:immunology*; D009500:Neutralization Tests; D011830:Radiation Effects; D018139:Simplexvirus / Q000276:immunology / Q000528:radiation effects*; D014185:Transplantation, Isogeneic; D014466:Ultraviolet Rays
D000914:Antibodies, Viral; D000956:Antigens, Viral; D004273:DNA, Neoplasm; D004279:DNA, Viral