title: Elevation of adenosine 3',5'-cyclic monophosphate in established mamalian cell strains by Hypaque (sodium diatrizoate).
abstract: Hypaque (sodium diatrizoate), when added to cells growing in culture (two HeLa S3 strains, rat glioblastoma C6 and mouse lymphoma L5178Y), increased the intracellular level of adenosine 3',5'-cyclic monophosphate. A transient elevation of adenosine 3',5'-cyclic monophosphate was also observed when L5178Y cells were subjected to a procedure recommended for separation of lymphocytes from peripheral blood. The effect of Hypaque did not appear to be related to the increase in osmolality of the medium.
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D002460:Cell Line; D003470:Culture Media; D000242:Cyclic AMP / Q000378:metabolism*; D003907:Dexamethasone / Q000494:pharmacology; D003973:Diatrizoate / Q000494:pharmacology*; D006367:HeLa Cells; D009994:Osmolar Concentration; D012965:Sodium Chloride / Q000494:pharmacology; D013395:Sucrose / Q000494:pharmacology
chemical_list: D003470:Culture Media; D003973:Diatrizoate; D012965:Sodium Chloride; D013395:Sucrose; D003907:Dexamethasone; D000242:Cyclic AMP; D000469:Alkaline Phosphatase