Interactions of the cholesterol side-chain with egg lecithin. A spin label study.
The effect in egg lecithin liposomes of cholesterol and cholesterol analogues with side-chains of reduced length on the order parameters of two steroid spin labels has been studied. Analogues with side-chains shorter than cholesterol by more than three carbon cause significantly less ordering than cholesterol. Liposomes containing a cholesterol analogue in which the side-chain is absent cause very little increase in the ordering of a new sterol spin label in which the nitroxide is incorporated into the side-chain. The results suggest that the sterol side-chain exerts a great influence on membrane rigidity within its immediate environment.
D001665:Binding Sites; D002784:Cholesterol; D004530:Egg Yolk; D004578:Electron Spin Resonance Spectroscopy; D005260:Female; D007700:Kinetics; D008081:Liposomes; D008954:Models, Biological; D008968:Molecular Conformation; D010713:Phosphatidylcholines; D013113:Spin Labels
D008081:Liposomes; D010713:Phosphatidylcholines; D013113:Spin Labels; D002784:Cholesterol