Purification and buoyant density of infectious bovine rhinotracheitis virus.
A purification scheme for infectious bovine rhinotracheitis virus utilizing rate-zonal centrifugation in a 10-40% potassium tartrate gradient was described. The density of IBRV in the potassium tartrate gradient was found to be 1.22 g/cm3. Electron microscopic examination of purified virus preparations revealed homogeneous populations of enveloped virions with minute projections on the envelope surface.
D002500:Centrifugation, Isopycnic; D007242:Herpesvirus 1, Bovine / Q000032:analysis / Q000302:isolation & purification* / Q000648:ultrastructure; D008854:Microscopy, Electron