[Evaluation of lymphocyte transformation test: relationship between nucleus and cell sizes of PHA-stimulated lymphocyes (author's transl)].
A methodological comparision of evaluation of lymphocyte transformation test is described. Three experts examined smear-slides of cells from five lymphocyte cultures and the corresponding controls in a blind study. In the morphological differentiation of 100 cells per slide in comparison to 1 000 cells per slide it was found a good intraindividually but a unsatisfactory interindividually correlation. 1 000 cells from stimulated and non-stimulated cultures shows the greatest difference for the parameter "total cell size" and the lowest difference for the "nucleus plasma relation" by the planimetrically imeasurement.
D002467:Cell Nucleus / Q000276:immunology; D002478:Cells, Cultured; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology*; D010835:Phytohemagglutinins / Q000494:pharmacology*
D010835:Phytohemagglutinins
Zur Auswertung des Lymphozytentransformationstests: Zellkern- und Zell flächenrelationen PHA-stimulierter Lymphozyten.