Evaluation of dye exclusion and colony inhibition techniques for detection of polyoma-specific, cell-mediated immunity.
Cellular immunity directed against polyoma virus-induced antigen was observed with C3H/HeJ splenic lymphoid cells from mice sensitized by a short-term immunization schedule with syngeneic polyoma 4198 and 4198V tumor cells. Polyoma specificity of the response was shown by demonstration that splenic cells from DBA/2J animals with polyoma virus-induced tumors were cytotoxic for the C3H 4198 and 4198V cells, but not for the L-M cell, another cell line of C3H origin. The polyoma-specific response in the syngeneic system was detectable with the dye exclusion assay but not with the colony inhibition procedure. Colony inhibition with the L-M cell was observed with sensitized lymphoid cells in both allogeneic and syngeneic systems.
D000818:Animals; D000918:Antibody Specificity; D000956:Antigens, Viral; D002455:Cell Division; D002460:Cell Line; D002999:Clone Cells; D005069:Evaluation Studies as Topic; D007111:Immunity, Cellular; D007158:Immunologic Techniques; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008809:Mice, Inbred C3H; D009374:Neoplasms, Experimental / Q000276:immunology; D011120:Polyomavirus / Q000276:immunology*; D013154:Spleen / Q000166:cytology; D014343:Trypan Blue
D000956:Antigens, Viral; D014343:Trypan Blue