[Standardization of "exchange" techniques to measure estradiol receptors in extracts exposed to biospecific adsorbants].
The measurement of the binding of steroid hormone receptors to biospecific adsorbants requires the development of "exchange" techniques. Two types of techniques based on the principle of differential dissociation were standardized: solid phase exchange with hydroxylapatite and liquid phase exchange with adsorption of the ligand to charcoal dextran. In both cases, binding of oestradiol to its receptor was measured in calf uterine cytosol extracts. Results obtained by both techniques were comparable. The half-times of the oestradiol-receptor complex dissociation determined from exchange kinetics are on the same order as those obtained by direct methods used in previous studies.
D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002846:Chromatography, Affinity / Q000379:methods; D003600:Cytosol / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D007700:Kinetics; D011956:Receptors, Cell Surface; D012996:Solutions; D014599:Uterus / Q000378:metabolism* / Q000648:ultrastructure
D011956:Receptors, Cell Surface; D012996:Solutions; D004958:Estradiol
Standardisation de techniques d'"échange" permettant de mesurer le récepteur de l'oestradiol dans des extraits mis en contact avec des adsorbants biospécifiques