The regulation of glycerol 3-phosphate oxidase of rate brownadipose tissue mitochondria by long-chain free fatty acids.
Added free fatty acids inhibit oxidation of glycerol 3-phosphate, succinate and NADH in brown-adipose tissue mitochondria from 10-day-old rats. The most pronounced is the inhibitory effect of glycerol 3-phosphate cytochrome c reductase (GP-cyto. c reductase). Contrary to other reductases, GP-cyto. c reductase activity of freshly isolated mitochondria is already inhibited by the fraction of endogenous free fatty acids. Both added and endogenous free fatty acids inhibition of GP-cyto. c reductase is fully reversible by the removal of free fatty acids by bovine serum albumine treatment. The inhibition of GP-cyto. c reductase is of strictly non-competitive type. The most inhibitory are unsaturated long-chain free fatty acids-oleic and linoleic acid. Results are discussed with regards to the regulatory importance of free fatty acids in brown-adiposetissue during intensive non-shivering thermogenesis.
D002001:Adipose Tissue, Brown / Q000187:drug effects / Q000201:enzymology*; D000818:Animals; D003065:Coenzyme A; D003579:Cytochrome Reductases / Q000378:metabolism; D003574:Cytochrome c Group; D005230:Fatty Acids, Nonesterified / Q000494:pharmacology*; D005260:Female; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism*; D008297:Male; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D009829:Oleic Acids / Q000494:pharmacology; D051381:Rats; D013384:Succinate Cytochrome c Oxidoreductase / Q000378:metabolism
D003574:Cytochrome c Group; D005230:Fatty Acids, Nonesterified; D009829:Oleic Acids; D013384:Succinate Cytochrome c Oxidoreductase; D005993:Glycerolphosphate Dehydrogenase; D003579:Cytochrome Reductases; D003065:Coenzyme A