Heterogeneity of surface antigens on endogenous type C virus-producing cell sublines derived from a clonal line of BALB/3T3 cells.
When viral envelope antigens (VEAs) and virus-associated cell surface antigens (CSAs) are used as markers of type C viruses, more heterogeneity of virus populations can be demonstrated than by using host range alone. In the present study, four CSAs (PC1, X.1, GCSA, and MEV-SA1) and three VEAs (xVEA, x1-VEA, and sub-gsVEA) were used as markers of virus populations present in various sublines of the BALB/c embryonic fibroblast BALB/3T3 clone A31 line. Of four spontaneously transformed sublines, two released N-tropic endogenous type C viruses spontaneously after long-term culture and each had distinct antigenic patterns. Treatment with 5-bromodoxyuridine (BrdU) resulted in the detection of X-tropic viruses in all four sublines. The expression of these X-tropic viruses was associated with two different antigenic patterns. When the clonal rabbit corneal SIRC cell line was infected with the X-tropic viruses obtained after BrdU treatment, the cells acquired detectable amounts of only one of the antigenic markers.
D000818:Animals; D015478:Antigens, Heterophile / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D002471:Cell Transformation, Neoplastic; D003315:Cornea; D005347:Fibroblasts; D051379:Mice; D008807:Mice, Inbred BALB C / Q000196:embryology; D011817:Rabbits; D012190:Retroviridae / Q000276:immunology* / Q000302:isolation & purification
D015478:Antigens, Heterophile; D000956:Antigens, Viral; D001973:Bromodeoxyuridine