Fetal properties in red blood cells of newborn infants.
In order to identify the fetal features in neonatal erythrocytes, cord blood was separated into seven fractions of varying specific density. Cell age in the single fractions was ascertained by means of reticulocyte count, glutamic-oxalacetic transaminase activity, and hemoglobin F concentration. The same procedures were used with blood of adults. With the exception of the fraction of neonatal blood with the highest specific density, the blood from neonates and adults correlated well for cell age and specific density. The highest specific density fraction of neonatal blood was found to contain a higher proportion of younger cells. The comparison of enzyme activities in the single fractions between neonates and adults showed that a high activity of glucose-6-phosphatedehydrogenase and enolase and a low activity of phosphofructokinase are typical fetal signs of neonatal cells.
D000328:Adult; D001219:Aspartate Aminotransferases / Q000097:blood; D002499:Centrifugation, Density Gradient; D004905:Erythrocyte Aging; D004906:Erythrocyte Count; D004912:Erythrocytes / Q000166:cytology* / Q000201:enzymology; D005312:Fetal Blood / Q000166:cytology*; D005319:Fetal Hemoglobin / Q000032:analysis; D005954:Glucosephosphate Dehydrogenase / Q000097:blood; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D010732:Phosphofructokinase-1 / Q000097:blood; D010751:Phosphopyruvate Hydratase / Q000097:blood
D005319:Fetal Hemoglobin; D005954:Glucosephosphate Dehydrogenase; D001219:Aspartate Aminotransferases; D010732:Phosphofructokinase-1; D010751:Phosphopyruvate Hydratase