Factors determining successful liver preservation for transplantation.
Auxiliary liver allotransplants will survive for relatively long periods of time after 24 hour hypothermic (10-12C), pulsatile perfusion. The best perfusate was a silica gel fraction of dog plasma with added potassium chloride gel made hyperosmolar with glucose. Further improvement could be achieved with added allopurinol and methylprednisolone. Nonpulsatile flow or lower temperatures were less effective preservation techniques.
D000493:Allopurinol / Q000494:pharmacology; D000641:Ammonia / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001663:Bilirubin / Q000097:blood; D003080:Cold Temperature; D003450:Cryoglobulins / Q000494:pharmacology; D004285:Dogs; D005260:Female; D005590:Fractional Precipitation; D005782:Gels; D005947:Glucose / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008099:Liver / Q000473:pathology; D016031:Liver Transplantation; D008297:Male; D008775:Methylprednisolone / Q000494:pharmacology; D009926:Organ Preservation / Q000379:methods*; D009994:Osmolar Concentration; D010477:Perfusion / Q000379:methods; D012709:Serum Albumin; D012822:Silicon Dioxide; D014021:Tissue Preservation / Q000379:methods*; D014184:Transplantation, Homologous
D003450:Cryoglobulins; D005782:Gels; D012709:Serum Albumin; D000493:Allopurinol; D012822:Silicon Dioxide; D000641:Ammonia; D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D005947:Glucose; D001663:Bilirubin; D008775:Methylprednisolone