title: Preparation of single cell suspensions from hepatoma cells in culture.
abstract: Fundamental examination was carried out on the liberation of single cells from hepatoma cells in culture. dRLa-74 cells derived from rat hepatoma, which hardly disperse as single cell suspensions with several proteolytic enzymes or EDTA alone, dispersed with a high yield of single cells by the combination of trypsin and EDTA. HUH-6 cells derived from human hepatoblastoma also showed similar results. The degree of cell dissociation by the combination was dependent on the incubation temperature or pH.
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D002460:Cell Line; D002469:Cell Separation / Q000379:methods*; D004492:Edetic Acid; D006801:Humans; D066298:In Vitro Techniques; D008113:Liver Neoplasms / Q000473:pathology*; D009374:Neoplasms, Experimental / Q000473:pathology; D051381:Rats; D014357:Trypsin
chemical_list: D004492:Edetic Acid; D014357:Trypsin