Affinity and specificity of antisera against human FSH and TSH obtained by immunizing rabbits with highly purified hormone preparations.
Two groups of 4 rabbits were immunized according to the method described by Vaitukaitis et al. (1971). One group was given 55 mu g (900 IU) FSH/animal and the other 100 mu g (o.5 IU) TSH/animal. Two highly specific antisera against FSH and one specific antiserum against TSH were obtained. The FSH antisera showed affinities towards LH and TSH of 0.13 and 0.25% respectively as compared to that of FSH. The TSH antisera showed affinities towards LH and FSH of 2.2 and 1.7% respectively as compared to that of TSH. The specific antisera showed no or minor affinities towards the LH-alpha subunit and they were sufficient specific to be used in the radioimmunoassay of serum from pregnant women without pre-absorption with HCG. Scatchard plots showed the association constants of the FSH antisera to be 1.3 times 10-11 and 1.2 times 10-11 1/mol respectively, and the association constant of the TSH antiserum to be 6 times 10-10 1/mol. All 3 antisera showed high titers.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D000918:Antibody Specificity; D001886:Bordetella pertussis; D006063:Chorionic Gonadotropin / Q000097:blood; D003429:Cross Reactions; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood / Q000276:immunology*; D006801:Humans; D007106:Immune Sera; D007114:Immunization; D007986:Luteinizing Hormone / Q000276:immunology*; D010567:Pertussis Vaccine; D011247:Pregnancy; D011261:Pregnancy Trimester, First; D011262:Pregnancy Trimester, Second; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay; D013972:Thyrotropin / Q000097:blood / Q000276:immunology*
D000906:Antibodies; D006063:Chorionic Gonadotropin; D007106:Immune Sera; D010567:Pertussis Vaccine; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D013972:Thyrotropin