Effect of aeration on the activity of gluconeogenetic enzymes in Saccharomyces cerevisiae growing under glucose limitation.
1. The effect of aeration on the key enzymes of gluconeogenesis was studied in baker's yeast (Saccharomyces cerevisiae) and in a nonrespiratory variant of S. cerevisiae grown under glucose limitation. 2. In baker's yeast phosphoenolpyruvate carboxykinase, hexosediphophatase and isocitrate lyase were completely repressed under anaerobic conditions. Their repression could be partially reversed by using intense aeration. 3. In the nonrespiratory variant these enzymes were absent independently of aeration. 4. Pyruvate carboxylase of baker's yeast showed maximal activity under anaerobic conditions. In the nonrespiratory variant pyruvate carboxylase had low activity under both anaerobic and aerobic conditions.
D000332:Aerobiosis; D000693:Anaerobiosis; D004794:Enzyme Repression; D006597:Fructose-Bisphosphatase / Q000096:biosynthesis; D005943:Gluconeogenesis; D005947:Glucose / Q000378:metabolism* / Q000494:pharmacology; D007523:Isocitrates; D007652:Oxo-Acid-Lyases / Q000096:biosynthesis; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000096:biosynthesis; D011766:Pyruvate Carboxylase / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
D007523:Isocitrates; D006597:Fructose-Bisphosphatase; D010729:Phosphoenolpyruvate Carboxykinase (GTP); D007652:Oxo-Acid-Lyases; D011766:Pyruvate Carboxylase; D005947:Glucose