title: The synthesis of the internucleotide (phosphodiester) bond by a base-catalysed reaction.
abstract: Potassium tert-butoxide in hexamethyl phosphoramide and dimethyl formamide provides an excellent catalyst for the reaction of a 3'-hydroxyl or a 5'-hydroxyl group of a nucleoside with an appropriate nucleoside phosphorofluoridate to yield the dinucleoside phosphate. This paper describes the experiments leading to the development of this reaction together with the synthesis of thymidylyl-(5' leads to 3')-thymidine (dT-dT).
mesh_terms: D008722:Methods; D009712:Nucleotides, Cyclic; D009841:Oligonucleotides / Q000138:chemical synthesis*; D010727:Phosphoric Diester Hydrolases; D010744:Phosphoric Monoester Hydrolases; D013942:Thymine Nucleotides
chemical_list: D009712:Nucleotides, Cyclic; D009841:Oligonucleotides; D013942:Thymine Nucleotides; D010744:Phosphoric Monoester Hydrolases; D010727:Phosphoric Diester Hydrolases