Estrogen binding protein of rat liver.
An estrogen binding protein for estradiol-17beta is present in the liver cytosol of female intact and one day oophorectomized rats. The dissociation constant reveals high affinity binding (Kd: 0.69 +/- 0.14 times 10(-10) M). Quantitation of EBP using a dextran-coated charcoal method shows that this specific macromolecular binding is much less than in the rat uterus, but similar to that in DMBA-induced mammary tumors. Sucrose density gradient analysis shows sedimentation at 8-9 S and 4-5 S when compared to bovine serum albumin.
D000818:Animals; D001665:Binding Sites; D002369:Castration; D003600:Cytosol / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D010053:Ovary / Q000502:physiology; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism; D051381:Rats; D011956:Receptors, Cell Surface
D011506:Proteins; D011956:Receptors, Cell Surface; D004958:Estradiol