title: Isotachophoresis: a new technique for determination of tissue metabolite concentrations.
abstract: An electrophoretic technique, isotachophoresis, has been adapted for qualitative and quantitative analysis of metabolites in human muscle tissue. Metabolite concentrations exceeding 0.1 nmol X kg-1 wet muslce could be determined in 5-10 mg muscle biopsy samples generally in good agreement with conventional analytical methods.
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D004586:Electrophoresis / Q000379:methods*; D005958:Glucosephosphates / Q000378:metabolism; D006358:Hot Temperature; D006801:Humans; D007773:Lactates / Q000378:metabolism; D009132:Muscles / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D010725:Phosphocreatine / Q000378:metabolism; D005082:Physical Exertion; D011773:Pyruvates / Q000378:metabolism
chemical_list: D005958:Glucosephosphates; D007773:Lactates; D010710:Phosphates; D011773:Pyruvates; D010725:Phosphocreatine; D009243:NAD; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate