Chick muscle in tissue culture: the ubiquity of viral infection.
In chick skeletal muscle fibers cultured from embryos of commercially obtained "normal" eggs we have demonstrated numerous C-particles and large amounts of avian leucosis/sarcoma envelope antigen, especially when cultured in the presence of dinitrophenol. C-particles were present in t-tubules, which were possible intracellular viaducts of infection or dissemination and perhaps were the loci of receptors of viral invasion of the cytoplasm and sites of egress. The abundant lace-like membraneous proliferations, probably of t-tubules, usually had C-particles adjacent to or within them and perhaps were virus-provoked. Questioned is the validity of using cultured muscle, or extract, of embryos from ordinary chicken eggs for analysing normal biological phenomena--or conversely, is viral influence "normal" in chick development?
D001355:Alpharetrovirus / Q000276:immunology; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002642:Chick Embryo; D002645:Chickens; D046508:Culture Techniques; D004140:Dinitrophenols; D008854:Microscopy, Electron; D009132:Muscles; D012190:Retroviridae / Q000032:analysis; D014780:Viruses / Q000032:analysis*
D000956:Antigens, Viral; D004140:Dinitrophenols