Subcellular localization of pulmonary antiotensin-converting enzyme (kininase II).
Goat antibodies to pig lung angiotensin-converting enzyme (kininase II) were conjugated to microperoxidase. Rat lung tissue, previously incubated with non-immune goat serum, was incubated with the antibody-microperoxidase conjugate and then with H2O2 and 3,3-diaminobenzidine. Electron microscopy revealed reaction product on the plasma membrane and caveolae of endothelial cells, especially those of capillaries and venules. These results support the hypothesis that angiotensin I and bradykinin are metabolized by enzymes on the luminal surface of pulmonary endothelial cells.
D000804:Angiotensin II / Q000032:analysis; D000818:Animals; D000906:Antibodies; D001920:Bradykinin / Q000032:analysis; D002462:Cell Membrane / Q000201:enzymology / Q000648:ultrastructure; D004727:Endothelium / Q000648:ultrastructure; D005260:Female; D006041:Goats / Q000276:immunology; D008168:Lung / Q000201:enzymology*; D008854:Microscopy, Electron; D007703:Peptidyl-Dipeptidase A / Q000032:analysis*; D010544:Peroxidases
D000906:Antibodies; D000804:Angiotensin II; D010544:Peroxidases; D007703:Peptidyl-Dipeptidase A; D001920:Bradykinin