Electron microscope localization of a protein bound near the origin of simian virus 40 DNA replication.
A salt-stable complex of protein and viral DNA obtained from Simian virus 40 (SV40)-infected monkey cells or mature SV40 virions has a novel structure. When viewed by high resolution electron microscopy, the circular SV40 DNA molecule has bound to it one to three globular protein "knobs". Using ecoRI and hpaII restriction endonucleases, each of which can cleave SV40 DNA once at a known location (10, 11, 12, 14), the bound protein can be localized at 0.7 plus or minis 0.05 on the SV40 DNA physical map (SV40 fractional length, clockwise from the ecoRI endonuclease-cleavage site).
D000818:Animals; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D004270:DNA, Circular; D004279:DNA, Viral; D004720:Endonucleases / Q000378:metabolism; D000882:Haplorhini; D007668:Kidney; D008854:Microscopy, Electron; D009698:Nucleoproteins; D011092:Polyethylene Glycols; D011402:Pronase / Q000378:metabolism; D013539:Simian virus 40 / Q000648:ultrastructure*; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014764:Viral Proteins / Q000302:isolation & purification
D004270:DNA, Circular; D004279:DNA, Viral; D009698:Nucleoproteins; D014764:Viral Proteins; D014316:Tritium; D011092:Polyethylene Glycols; D004720:Endonucleases; D011402:Pronase; D013936:Thymidine