title: The role of acidic proteins from cytoplasmic fractions of Krebs II ascites cells for efficient translation.
abstract: Acidic proteins with affinity to RNA from cytoplasmic fraction of Krebs II Ascites cells were isolated by means of affinity chromatography on RNA-Sepharose CN-Br-columns. Stepwise elution with 350 mM [K+] and 1000 mM [K+] removed two fractions of proteins both of which are required for the formation of 40S- or 80S-initiation complexes and for efficient translation.
mesh_terms: D000818:Animals; D002287:Carcinoma, Krebs 2 / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D004273:DNA, Neoplasm / Q000378:metabolism; D000882:Haplorhini; D006367:HeLa Cells / Q000378:metabolism; D007700:Kinetics; D051379:Mice; D008807:Mice, Inbred BALB C; D010442:Peptide Chain Initiation, Translational; D010445:Peptide Elongation Factors / Q000302:isolation & purification / Q000378:metabolism*; D010448:Peptide Initiation Factors / Q000302:isolation & purification / Q000378:metabolism*; D011132:Polyribosomes / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D004273:DNA, Neoplasm; D010445:Peptide Elongation Factors; D010448:Peptide Initiation Factors; D012333:RNA, Messenger