Role of gene 59 of bacteriophage T4 in repair of UV-irradiated and alkylated DNA in vivo.
Nonsense mutants in gene 59 (amC5, amHL628) were used to study the role of this gene in the repair of UV-damaged and alkylated DNA of bacteriophage T4 in vivo. The higher sensitivity to UV irradiation and alkylation of gene 59 mutants after exposure to these agents was established by a comparison of the survival fractions with wild type. Zonal centrifugal analysis of both parental and nascent mutant intracellular DNA molecules after UV irradiation showed that immediately after exposure the size of single-stranded DNA fragments was the same as the wild-type intracellular DNA. However, the capability of rejoining fragmented intracellular DNA was greatly reduced in the mutant. In contrast, the wild-type-infected cells under the same condition resumed DNA replication and repaired its DNA to normal size. Methyl methanesulfonate induced more randomly fragmented intracellular DNA, when compared to UV irradiation. The rate of rejoining under these conditions as judged from their sedimentation profiles was also greatly reduced in mutant-infected cells. Further evidence is presented that UV repair is not a simple consequence of arrested DNA replication, which is a phenotype of the mutant when infected in a nonpermissive host, Escherichia coli B (su minus), but rather that the DNA repair function of gene 59 is independent of the replication function. These and other data presented indicate that a product(s) of gene 59 is essential for both repair of UV lesions and repair of alkylation damage of DNA in vivo. It is suggested that gene 59 may have two functions during viral development: DNA replication and replication repair of DNA molecules.
D000478:Alkylation; D002499:Centrifugation, Density Gradient; D003090:Coliphages / Q000254:growth & development / Q000378:metabolism*; D004260:DNA Repair / Q000187:drug effects; D004261:DNA Replication / Q000187:drug effects; D004267:DNA Viruses; D004279:DNA, Viral / Q000096:biosynthesis / Q000378:metabolism / Q000528:radiation effects; D005796:Genes; D008698:Mesylates / Q000494:pharmacology; D008970:Molecular Weight; D009154:Mutation; D011830:Radiation Effects; D014466:Ultraviolet Rays; D014779:Virus Replication / Q000528:radiation effects
D004279:DNA, Viral; D008698:Mesylates