Effect of cyclic nucleotides on the cyanide-insensitive respiration of polymorphonuclear leucocytes.
The effects of N6,O2'-dibutyryladenosine 3',5'-monophosphate (Bu2-cyclic AMP) and N2,O2'-dibutyrylguanosine 3',5'-monophosphate (Bu2-cyclic GMP) on the cyanide-insensitive respiration of guinea pig peritoneal exudate polymorphonuclear leucocytes were studied. Bu2-cyclic AMP inhibited the respiration induced both by phagocytosis of E. coli and by the interaction with trypsin-digested rat liver microsomes. The addition of theophylline gave rise to an inhibitory pattern similar to that with Bu2-cyclic AMP against both the respirations induced. On the other hand, Bu2-cyclic GMP did not affect the respiration induced by phagocytosis whereas it inhibited the respiration induced by the addition of myristic acid was inhibited by Bu2-cyclic AMP, which was similar to that with E. coli. The respiration induced by methylene blue was inhibited neither by Bu2-cyclic AMP nor by Bu2-cyclic GMP. These observations suggest that the cyanide-insensitive respiration of polymorphonuclear leucocytes may be classified into at least three types from the inhibitory pattern of cyclic AMP and cyclic GMP.
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D003486:Cyanides / Q000494:pharmacology*; D000242:Cyclic AMP / Q000494:pharmacology; D006152:Cyclic GMP / Q000494:pharmacology; D004926:Escherichia coli; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007962:Leukocytes / Q000187:drug effects / Q000378:metabolism*; D008751:Methylene Blue / Q000494:pharmacology; D008862:Microsomes, Liver / Q000502:physiology; D009227:Myristic Acids / Q000494:pharmacology; D009712:Nucleotides, Cyclic / Q000494:pharmacology*; D010101:Oxygen Consumption / Q000187:drug effects*; D010587:Phagocytosis / Q000187:drug effects; D051381:Rats; D013806:Theophylline / Q000494:pharmacology; D014357:Trypsin / Q000494:pharmacology
D003486:Cyanides; D009227:Myristic Acids; D009712:Nucleotides, Cyclic; D003994:Bucladesine; D013806:Theophylline; D000242:Cyclic AMP; D014357:Trypsin; D006152:Cyclic GMP; D008751:Methylene Blue