Genomic complexities of murine leukemia and sarcoma, reticuloendotheliosis, and visna viruses.
The genetic complexities of several ribodeoxyviruses were measured by quantitative analysis of unique RNase T1-resistant oligonucleotides from 60-70S viral RNAs. Moloney murine leukemia virus was found to have an RNA complexity of 3.5 x 10(6) daltons, whereas Moloney murine sarcoma virus had a significantly smaller genome size of 2.3 x 10(6). Reticuleondotheliosis and visna virus RNAs had complexities of 3.9 x 10(6), respectively. Analysis of RNase A-resistant oligonucleotides of Rous sarcoma virus RNA gave a complexity of 3.6 x 10(6), similar to that previously obtained with RNase T1-resistant oligonucleotides. Since each of these viruses was found to have a unique sequence genomic complexity near the molecular weight of a single 30-40S viral RNA subunit, it was concluded that ribodeoxyvirus genomes are at least largely polyploid.
D001483:Base Sequence; D017988:Gammaretrovirus / Q000032:analysis*; D009841:Oligonucleotides / Q000032:analysis; D012328:RNA Viruses / Q000032:analysis*; D001356:Reticuloendotheliosis virus / Q000032:analysis*; D012190:Retroviridae / Q000032:analysis*; D012260:Ribonucleases / Q000378:metabolism; D009053:Sarcoma Viruses, Murine / Q000032:analysis; D014790:Visna-maedi virus / Q000032:analysis*
D009841:Oligonucleotides; D012260:Ribonucleases