title: Altered deoxyribonucleotide pools in P2 eductants of Escherichia coli K-12 due to deletion of the dcd gene.
abstract: Deletion of the Escherichia coli K-12 chromosome associated with P2 mediated education extend through the structural gene for uridine kinase, udk, and the dcd gene encoding 2'-deoxycytidine 5'-triphosphate deaminase. The lack of uridine kinase makes a positive selection possible for these strains. Due to the dcd mutation, P2 eductants show large alterations in their deoxyribonucleoside triphosphate pools.
mesh_terms: D003854:Deoxyribonucleotides / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism*; D005796:Genes; D006639:Histidine / Q000378:metabolism; D009154:Mutation; D009709:Nucleotide Deaminases / Q000096:biosynthesis*; D010770:Phosphotransferases / Q000096:biosynthesis*; D014161:Transduction, Genetic; D014541:Uridine Kinase / Q000096:biosynthesis*
chemical_list: D003854:Deoxyribonucleotides; D006639:Histidine; D010770:Phosphotransferases; D014541:Uridine Kinase; D009709:Nucleotide Deaminases