Effects of mitogens on glucose oxidation by lymphocytes from normal individuals and patients with chronic lymphocytic leukemia.
The effects of phytohemagglutinin (PHA), concanavalin a (con A) and endotoxin on glucose oxidation by lymphocytes from normal individuals and patients with chronic lymphocytic leukemia (Cll) were demonstrated without using tissue culture techniques. PHA and CON A had no effect on glucose-6-14C oxidation, but stimulated glucose-1-14C oxidation by normal lmphocytes. This stimulation of glucose-1-14C oxidation occurred within 1 hour and could be prevented by colchicine. In contrast, endotoxin had no effect on either gucose-I-14C or glucose-6-14C oxidation by normal lymphocytes. Lymphocytes from five out of six Cll patients showed low basal rate of gucose-1-14C oxidation by the lymphocytes of these five patients.
D002731:4-Chloromercuribenzenesulfonate / Q000494:pharmacology; D000368:Aged; D003078:Colchicine / Q000494:pharmacology; D003208:Concanavalin A / Q000037:antagonists & inhibitors / Q000494:pharmacology; D004336:Drug Antagonism; D004731:Endotoxins / Q000494:pharmacology; D005260:Female; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D037102:Lectins / Q000494:pharmacology; D007945:Leukemia, Lymphoid / Q000378:metabolism*; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D008274:Magnesium / Q000494:pharmacology; D008875:Middle Aged; D008934:Mitogens / Q000494:pharmacology*; D010084:Oxidation-Reduction; D013268:Stimulation, Chemical
D004731:Endotoxins; D037102:Lectins; D008934:Mitogens; D003208:Concanavalin A; D002731:4-Chloromercuribenzenesulfonate; D008274:Magnesium; D005947:Glucose; D003078:Colchicine