title: Biochemical properties of simian virus 40-transformed 3T3 cell mitochondria.
abstract: Mitrochondria isolated from simian virus 40-transformed 3T3 and nontransformed 3T3 cells were compared by various biochemical criteria. Transformed and nontransformed cell mitochondria had identical densities in linear sucrose and discontinuous bovine serum albumin gradients. The activities of several mitochondria-specific enzymes including cytochrome oxidase, adenylate kinase, nicotinamide adenine dinucleotide (NADH)-cytochrome c reductase, and NADH oxidase were similar in both cell types. However, the activity of the mitochondrial outer membrane enzyme, monoamine oxidase. In the virus-transformed cell mitochondria was reduced to 50% of that in nontransformed cell mitochondria.
mesh_terms: D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D003579:Cytochrome Reductases / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000378:metabolism; D005347:Fibroblasts / Q000201:enzymology / Q000648:ultrastructure*; D008566:Membranes / Q000201:enzymology; D051379:Mice; D008928:Mitochondria / Q000201:enzymology*; D008995:Monoamine Oxidase / Q000378:metabolism; D010770:Phosphotransferases / Q000378:metabolism; D013539:Simian virus 40
chemical_list: D008995:Monoamine Oxidase; D003579:Cytochrome Reductases; D003576:Electron Transport Complex IV; D010770:Phosphotransferases