title: In vitro cultivation of cells from the chorioallantoic membrane of chick embryos.
abstract: By treatment of chorioallantoic membranes from embryonated eggs with collagenase and hyaluronidase before the conventional application of trypsin cells could be grown in culture which supported growth of a large variety of myxoviruses, herpesviruses, avian reoviruses and the infectious bronchitis virus of chickens. The cultures could be used for sensitive plaque assays and neutralization tests.
mesh_terms: D000482:Allantois / Q000166:cytology*; D000818:Animals; D002469:Cell Separation; D002478:Cells, Cultured / Q000382:microbiology*; D002642:Chick Embryo / Q000166:cytology*; D002645:Chickens / Q000276:immunology; D002823:Chorion / Q000166:cytology; D003332:Coronaviridae / Q000254:growth & development; D003588:Cytopathogenic Effect, Viral; D005321:Extraembryonic Membranes / Q000166:cytology*; D005347:Fibroblasts / Q000382:microbiology; D006564:Herpesviridae / Q000254:growth & development; D006736:Horses; D006801:Humans; D006821:Hyaluronoglucosaminidase / Q000494:pharmacology; D007106:Immune Sera; D003012:Microbial Collagenase / Q000494:pharmacology; D009500:Neutralization Tests; D009975:Orthomyxoviridae / Q000254:growth & development; D010252:Paramyxoviridae / Q000254:growth & development; D011817:Rabbits / Q000276:immunology; D012087:Reoviridae / Q000254:growth & development; D014357:Trypsin / Q000494:pharmacology; D010948:Viral Plaque Assay; D014776:Virus Cultivation; D014779:Virus Replication
chemical_list: D007106:Immune Sera; D006821:Hyaluronoglucosaminidase; D014357:Trypsin; D003012:Microbial Collagenase