A microculture technique for rat lymphocyte transformation.
We report the development of an economical microculture technique suitable for measuring rat lymphocyte response to mitogens and in mixed lymphocyte reactions. The effects of varying culture conditions, i.e. source of serum, addition and concentration of 2-mercaptoethanol, mitogen concentrations, culture incubation times, absorption of serum, lymphocyte numbers and microtitre plate well shape are described.
D000042:Absorption; D000818:Animals; D002417:Cattle; D002452:Cell Count; D003208:Concanavalin A / Q000494:pharmacology; D004306:Dose-Response Relationship, Immunologic; D006358:Hot Temperature; D006801:Humans; D007158:Immunologic Techniques; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008623:Mercaptoethanol / Q000494:pharmacology; D008722:Methods; D010835:Phytohemagglutinins / Q000494:pharmacology; D011043:Pokeweed Mitogens / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000276:immunology; D013997:Time Factors
D010835:Phytohemagglutinins; D011043:Pokeweed Mitogens; D003208:Concanavalin A; D008623:Mercaptoethanol