Improved synthesis and rapid isolation millimole quantities of adenylysulfate.
An improved enzymatic method for the synthesis of adenylysulfate (APS) from adenosine 5'-phosphate using APS-reductase from Thiobacillus denitrificans is described. Isolation of millimole quantitities of this sulfur nucleotide is achieved rapidly by means of ion exchange chromatography on a strongly basic ion exchange resin. A facile and reproducible desalting procedure is described.
D000249:Adenosine Monophosphate / Q000031:analogs & derivatives*; D000250:Adenosine Phosphosulfate / Q000096:biosynthesis* / Q000302:isolation & purification; D010088:Oxidoreductases; D013855:Thiobacillus / Q000201:enzymology
D000249:Adenosine Monophosphate; D000250:Adenosine Phosphosulfate; D010088:Oxidoreductases