[Fluorimetric ultramicrodetermination of the dansyl derivatives of cyclic 3'5-AMP by thin-layer chromatography].
A new fluoriemtric ultramicromethod was developed for quantiative determination of cyclic 3',5'-AMP as derivative of dansyl chloride using silica gel thin-layer chromatography. In presence of dansyl chloride cyclic 3',5'-AMP formed a fluorescent complex. A spot of dansyl derivative of cyclic 3',5'-AMP was photographed on a reversible colour film, after which the colour intensity of the spot on the film was determined directly by a special densitometer. Content of cyclic 3',5'-AMP was studied in different regions of rat brain. The in fluence of some psychotropic drugs on the level of cyclic 3',5'-AMP was studied using this method. The method was highly sensitive and reproducible and enabled to determine 10(-10) -- 10(-11) M cyclic 3',5'-AMP.
D002855:Chromatography, Thin Layer / Q000379:methods; D000242:Cyclic AMP / Q000032:analysis*; D003619:Dansyl Compounds; D008832:Microchemistry; D013050:Spectrometry, Fluorescence / Q000379:methods
D003619:Dansyl Compounds; D000242:Cyclic AMP
Fliuroimenrichedkoe ul' tramikroopridilenie dansal-proizvodnogo tsiklicheskoĭ 3'5'-adenozinmonofosfornoĭ kisloty (3'5'-AMF) metodom tonkosloinoĭ khromatografii