Determination of molar ratios of vesicular stomatitis virus induced RNA species in BHK21 cells.
A modified procedure for analysis of RNA in denaturing formamide-polyacrylamide slab gels containing 6 M urea is described. Using this technique, in conjucntion with fluorographic analysis, we determined molecular weights and molar ratios of the various vesicular stomatitis virus (VSV) induced RNAs in BHK21 cells. A comparison of the molar ratios of virus-specific mRHAs and their putative protein products in these cells suggests that there is little, if any, translational control of viral gene expression during acute VSV infection.
D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D005796:Genes; D008970:Molecular Weight; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis*; D012367:RNA, Viral / Q000096:biosynthesis*; D014158:Transcription, Genetic; D014721:Vesicular stomatitis Indiana virus / Q000378:metabolism*
D012367:RNA, Viral; D012313:RNA