A kinetic method to establish the specificity of spin labelling of macromolecules.
A method has been devised for establishing the specificity of spin labelling of a macromolecule. Analysis of the rate of incorporation of spin label under different conditions permits the assignment of reacting groups to classes of differing reactivity. The method is illustrated for the enzyme glycogen phosphorylase b.
D004578:Electron Spin Resonance Spectroscopy; D007700:Kinetics; D046911:Macromolecular Substances; D008970:Molecular Weight; D006005:Phosphorylases / Q000032:analysis / Q000302:isolation & purification; D011506:Proteins / Q000032:analysis; D012588:Scintillation Counting; D013113:Spin Labels
D046911:Macromolecular Substances; D011506:Proteins; D013113:Spin Labels; D006005:Phosphorylases