title: Effect of gold sodium thiomalate on fibrinolysis.
abstract: Effects of gold sodium thiomalate (GST) on fibrinolytic activities of normal blood and plasma, polymorphonuclear leukocytes (PMN), purified PMN fibrinolytic enzymes, and the enzyme, plasmin, were examined by 125I-fibrin radiometric assay. Of the 2 enzymes accounting for all of the fibrinolytic activity extractable from PMN, the chymotrypsin-like enzyme (cathepsin G),but not the elastase, was inhibited by GST (50% inhibition at 10-6M, 80% inhibition at 10-5M). Plasmin was not inhibited by GST (10-4M to 10-7 M), and these concentrations had no effect on fibrinolytic activities of normal blood and plasma (6 subjects). Activities of PMN preparations from only 2 of 5 normal subjects were inhibited by GST (maximum inhibition of 29% at 10-5 M). These findings indicate a differential effect of GST on PMN elastase and cathepsin G, and suggest a minor role for the latter in normal PMN fibrinolytic activity.
mesh_terms: D002403:Cathepsins / Q000378:metabolism; D005341:Fibrinolysin / Q000378:metabolism; D005342:Fibrinolysis / Q000187:drug effects*; D006052:Gold Sodium Thiomalate / Q000494:pharmacology*; D006801:Humans; D007962:Leukocytes / Q000201:enzymology; D010196:Pancreatic Elastase / Q000378:metabolism; D014568:Urokinase-Type Plasminogen Activator / Q000494:pharmacology
chemical_list: D006052:Gold Sodium Thiomalate; D002403:Cathepsins; D010196:Pancreatic Elastase; D005341:Fibrinolysin; D014568:Urokinase-Type Plasminogen Activator