title: Ribonuclease A digestion by proteinase K.
abstract: The digestion of ribonuclease A by proteinase K yielded one major degradation product only, which could not be distinguished from ribonuclease S by electrophoretical and immunological methods. This component (ribonuclease K) possessing full catalytic activity was characterized to be (1--20/21--124) ribonuclease A. Combined action of proteinase K and trypsin on ribonuclease A leads to a significant increase of the inactivation rate which may be useful in the isolation of mRNA from polysomes.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D010450:Endopeptidases / Q000378:metabolism*; D007700:Kinetics; D003904:Mitosporic Fungi / Q000201:enzymology; D012260:Ribonucleases / Q000378:metabolism
chemical_list: D000596:Amino Acids; D012260:Ribonucleases; D010450:Endopeptidases