In vitro interaction of estrogen and prolactin on hormone-dependent rat mammory tumors (38511).
Hormone-dependent, regressing mammary tumors in rats were incubated in a chemically defined culture medium containing tritium labeled leucine at 37 degrees for 2 hr. The rate of tritium incorporation into the protein fraction by these tissues was measured with and without the addition of 17beta-estradiol and/or ovine prolactin into the medium. Tumor tissue responded to either estrogen or porlactin by a 6-7% increase in the rate of 3-H-leucine incorporation. When the incubation medium contained both the  hormones, the increase in the rate of 3-H leucine incorporation was 19% which was significantly greater than the sum of the effect of each single hormone measured independelntly. These results demonstrate a synergistic effect between estrogen and prolactin on rat mammary tumors.
D000818:Animals; D001551:Benz(a)Anthracenes; D002273:Carcinogens; D004357:Drug Synergism; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D007930:Leucine / Q000378:metabolism; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced*; D009363:Neoplasm Proteins / Q000096:biosynthesis; D011388:Prolactin / Q000494:pharmacology*; D051381:Rats; D011956:Receptors, Cell Surface
D001551:Benz(a)Anthracenes; D002273:Carcinogens; D009363:Neoplasm Proteins; D011956:Receptors, Cell Surface; D004958:Estradiol; D011388:Prolactin; D007930:Leucine