title: Isolation and properties of brain alpha-actinin.
abstract: alpha-Actinin isolated from bovine brain migrated on sodium dodecyl sulphate/polyacrylamide-gel electrophoresis like muscle alpha-actinin with an apparent mol.wt. of 100000 and cross-reacted with antibodies to muscle alpha-actinin. Brain alpha-actinin modulated actin-myosin Mg2+-activated adenosine triphosphatase activity and, when bound by polystyrene particles, was found to bind muscle actin and tropomyosin from solution. Brain alpha-actinin, in conjunction with the other components of the contractile and relaxing complex, may play a role in the release of neurotransmitters from synaptic vesicles.
mesh_terms: D000185:Actinin / Q000302:isolation & purification / Q000378:metabolism*; D000205:Actomyosin / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002417:Cattle; D002499:Centrifugation, Density Gradient; D004591:Electrophoresis, Polyacrylamide Gel; D005779:Immunodiffusion; D008274:Magnesium / Q000494:pharmacology; D009124:Muscle Proteins / Q000378:metabolism*; D009132:Muscles / Q000378:metabolism; D011137:Polystyrenes; D013572:Synaptic Vesicles / Q000378:metabolism
chemical_list: D009124:Muscle Proteins; D011137:Polystyrenes; D000185:Actinin; D000205:Actomyosin; D000251:Adenosine Triphosphatases; D008274:Magnesium