Studies on the terminal stages of antibody-complement-mediated killing of a tumor cell. II. Inhibition of transformation of T to dead cells by 3'5' cAMP.
Transformation of T to dead cells was prevented by 3'5' cAMP. The effect of 3'5' cAMP was dose, time, and temperature dependent. T washed free of 3'5' cAMP after short-term incubation proceeded to die to the same extent as control cells. After 3 hr of incubation of T with 3'5' cAMP the level of killing was significantly reduced. The 3'5' cyclic nucleotides of uridine, guanine, cytosine, and thymidine and the 2'3' cyclic adenosine nucleotide were not effective. It was concluded that prolonged treatment of T with 3'5' cAMP either irreversibly blocked the damage-producing process or facilitated the reapir of damaged sites.
D000818:Animals; D000906:Antibodies; D006528:Carcinoma, Hepatocellular / Q000139:chemically induced; D002467:Cell Nucleus; D002470:Cell Survival; D003165:Complement System Proteins / Q000378:metabolism*; D000242:Cyclic AMP / Q000494:pharmacology*; D003601:Cytotoxicity Tests, Immunologic; D004052:Diethylnitrosamine; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008113:Liver Neoplasms; D013696:Temperature; D013997:Time Factors
D000906:Antibodies; D004052:Diethylnitrosamine; D003165:Complement System Proteins; D000242:Cyclic AMP