Reduced nicotinamide adenine dinucleotide-activated phosphoenolpyruvate carboxylase in Pseudomonas MA: potential regulation between carbon assimilation and energy production.
Comparison of enzyme activities in crude extracts of methylamine-grown Pseudomonas MA (ATCC 23319) to those in succinate-grown cells indicates the involvement of an acetyl coenzyme A-independent phosphoenolpyruvate carboxylase in one-carbon metabolism. The purified phosphoenolpyruvate carboxylase is activated specifically by reduced nicotinamide adenine dinucleotide (KA = 0.2 mM). The regulatory properties of this enzyme suggests that phosphoenolpyruvate serves as a focal point for both carbon assimilation and energy metabolism.
D000429:Alcohol Oxidoreductases / Q000378:metabolism; D002244:Carbon / Q000378:metabolism*; D002474:Cell-Free System; D004734:Energy Metabolism; D004789:Enzyme Activation; D005991:Glycerol Kinase / Q000378:metabolism; D012695:L-Serine Dehydratase / Q000378:metabolism; D008291:Malate Dehydrogenase / Q000378:metabolism; D008744:Methylamines / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000096:biosynthesis* / Q000378:metabolism; D010736:Phosphoglycerate Mutase / Q000378:metabolism; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D011549:Pseudomonas / Q000201:enzymology* / Q000378:metabolism; D011773:Pyruvates; D013386:Succinates / Q000378:metabolism
D008744:Methylamines; D011773:Pyruvates; D013386:Succinates; D009243:NAD; D002244:Carbon; D000429:Alcohol Oxidoreductases; D008291:Malate Dehydrogenase; D005991:Glycerol Kinase; D010729:Phosphoenolpyruvate Carboxykinase (GTP); D010751:Phosphopyruvate Hydratase; D012695:L-Serine Dehydratase; D010736:Phosphoglycerate Mutase