title: Protein and glycoprotein electrophoretic patterns of enriched fractions of primary and secondary granules from guinea pig polymorphonuclear leukocytes.
abstract: The postnuclear supernatant fraction of sucrose homogenates of guinea pig polymorphonuclear leukocytes (PMNL) was subjected to differential centrifugation to obtain a total particulate fraction, a particle-free supernatant fraction, highly enriched fractions of primary and secondary granules, and a membrane-rich fraction. The various fractions were solubilized in buffer containing sodium dodecyl sulfate (SDS) and analyzed for protein and glycoproteincomponents by SDS -polyacrylamide gel electrophoresis. The major glycoprotein components of the postnuclear supernatant fraction were found mainly associated with the enriched fraction of secondary granules and, to a lesser extent, with the membrane-rich fraction. No major glycoprotein components were visible in the polypeptide electrophoretic patterns of the primary granule fraction or of the particle-free supernate. Attempts at separation of guinea pig granules by zonal sucrose density gradient centrifugation were only partially successful. Data supporting a species difference in this regard between rabbit and guinea pig PMNL granules are presented.
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D002462:Cell Membrane / Q000032:analysis; D002499:Centrifugation, Density Gradient; D002501:Centrifugation, Zonal; D003594:Cytoplasmic Granules / Q000032:analysis* / Q000648:ultrastructure; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000032:analysis*; D006168:Guinea Pigs; D008854:Microscopy, Electron; D008858:Microscopy, Phase-Contrast; D009504:Neutrophils / Q000032:analysis* / Q000648:ultrastructure; D009195:Peroxidase / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D011817:Rabbits; D013045:Species Specificity; D013347:Subcellular Fractions / Q000032:analysis
chemical_list: D006023:Glycoproteins; D011506:Proteins; D009195:Peroxidase; D000469:Alkaline Phosphatase