The effects of cyclic adenosine 3',5'-monophosphate and other adenine nucleotides on body temperature.
1. Adenosine 3',5'-monophosphate (cAMP), its dibutyryl derivative (Db-cAMP) and other adenine nucleotides have been micro-injected into the hypothalamic region of the unanaesthetized cat and the effects on body temperature, and on behavioural and autonomic thermoregulatory activities observed. 2. Db-cAMP and cAMP both produced hypothermia when applied to the pre-optic anterior hypothalamus. With Db-cAMP the hypothermia was shown to be dose dependent between 50 and 500 mug (0-096-0-96 mumole). 3. AMP, ADP and ATP also produced hypothermia when injected into the pre-optic anterior hypothalamus. 4. The order of relative potencies of the adenine nucleotides with respect both to the hypothermia produced and to the autonomic thermoregulatory effects observed were similar. Db-cAMP was most potent and cAMP least. 5. Micro-injection into the pre-optic anterior hypothalamus of many substances including saline produced in most cats a non-specific rise in body temperature apparently the result of tissue damage. Intraperitoneal injection of 4-acetamidophenol (paracetamol 50 mg/kg) reduced or abolished this febrile response. 6. The hypothermic effect of the adenine nucleotides has been compared with the effects produced in these same cats by micro-injections of noradrenaline, 5-hydroxytryptamine, a mixture of acetylcholine and physostigmine (1:1), EDTA and excess Ca2+ ions. 7. It is concluded that as Db-cAMP and cAMP both produce hypothermia, it is unlikely that endogenous cAMP in the pre-optic anterior hypothalamus mediates the hyperthermic responses to pyrogens and prostaglandins.
D000082:Acetaminophen / Q000494:pharmacology; D000109:Acetylcholine / Q000494:pharmacology; D000227:Adenine Nucleotides / Q000494:pharmacology*; D000244:Adenosine Diphosphate / Q000494:pharmacology; D000249:Adenosine Monophosphate / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D001831:Body Temperature / Q000187:drug effects*; D001833:Body Temperature Regulation / Q000187:drug effects; D003994:Bucladesine / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D002415:Cats; D000242:Cyclic AMP / Q000494:pharmacology*; D004492:Edetic Acid / Q000494:pharmacology; D005260:Female; D007031:Hypothalamus / Q000187:drug effects; D007035:Hypothermia / Q000139:chemically induced; D009638:Norepinephrine / Q000494:pharmacology; D010830:Physostigmine / Q000494:pharmacology; D011301:Preoptic Area / Q000187:drug effects; D012701:Serotonin / Q000494:pharmacology
D000227:Adenine Nucleotides; D012701:Serotonin; D000082:Acetaminophen; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D003994:Bucladesine; D000255:Adenosine Triphosphate; D004492:Edetic Acid; D010830:Physostigmine; D000242:Cyclic AMP; D000109:Acetylcholine; D002118:Calcium; D009638:Norepinephrine