title: Different stimulating capacity of monocytes and B lymphocytes in mixed leukocyte cultures: a dose response study.
abstract: Cell fractions highly enriched either for monocytes of for B lymphocytes were separated from human peripheral blood. They were tested for their capacity to stimulate allogeneic lymphocytes in mixed leukocyte cultures, using dose-response titrations. Neither population could be stimulated to proliferate by allogeneic cells, but both proved strong stimulators. In this respect monocytes were more efficient than B cell preparations. Differences were pronounced at the lower numbers of stimulating cells but were no longer significant with large amounts of cells.
mesh_terms: D001402:B-Lymphocytes / Q000276:immunology*; D002469:Cell Separation; D003470:Culture Media; D005615:Freezing; D006801:Humans; D007958:Leukocyte Count; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D009000:Monocytes / Q000276:immunology*; D011947:Receptors, Antigen, B-Cell; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D003470:Culture Media; D011947:Receptors, Antigen, B-Cell