The influence of insulin on various enzyme activities in human and rat hepatoma cells.
1. Incubation of human and rat hepatoma cells with insulin (1 mU/10(6) cells) decreases their content of adenosine 3':5'-monophosphate by more than half after 1 h and by about a quarter after 4 h. 2. The activities of the ATP-metabolising enzymes, adenylate kinase and Mg2+-adenosine triphosphatase are significantly increased by insulin within 1 h and after 4 h. Activity of succinate dehydrogenase and lactic dehydrogenase showed no change at either time interval. 3. Insulin markedly stimulated glucose 6-phosphate dehydrogenase activity within 1 h but by 4 h the increase was less apparent. Glutamate dehydrogenase activity by contrast was not increased by 1 h but was elevated at 4 h.
D000249:Adenosine Monophosphate / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000263:Adenylate Kinase / Q000378:metabolism*; D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000201:enzymology* / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D005969:Glutamate Dehydrogenase / Q000378:metabolism*; D006801:Humans; D007328:Insulin / Q000494:pharmacology*; D007700:Kinetics; D008113:Liver Neoplasms / Q000201:enzymology* / Q000378:metabolism; D009374:Neoplasms, Experimental / Q000201:enzymology / Q000378:metabolism; D010770:Phosphotransferases / Q000378:metabolism*; D051381:Rats; D012710:Serum Albumin, Bovine / Q000494:pharmacology
D007328:Insulin; D012710:Serum Albumin, Bovine; D000249:Adenosine Monophosphate; D000242:Cyclic AMP; D005954:Glucosephosphate Dehydrogenase; D005969:Glutamate Dehydrogenase; D010770:Phosphotransferases; D000263:Adenylate Kinase; D000251:Adenosine Triphosphatases