Proliferation and metabolic activities of Ehrlich ascites tumor cells in chemically defined albumin media.
Cell proliferation, viability, DNA-, RNA-, protein synthesis, amino acid transport, repiration and lactate/glucose quotient of Ehrlich ascites tumor cells grown in suspension culture in serum free medium supplemented with albumin charges of different origin were studied. Optimal cell growth was obtained in nutrient medium supplemented with 1% bovine serum albumin (Cohn-fraction V, Serva). Cell proliferation under these culture conditions was delayed to 50% as compared to controls in normal medium; the rate of synthesis of macromolecules was reduced; energy metabolism was not significantly imparied. The trend of the cells in albumin medium to attach to glass was independent from the pH of the cultures between 7.2 and 8.0; it was enhanced by fatty acid deprivation of the albumin.
D000596:Amino Acids / Q000378:metabolism; D000621:Aminoisobutyric Acids / Q000378:metabolism; D000818:Animals; D001692:Biological Transport; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism / Q000503:physiopathology*; D002455:Cell Division; D002470:Cell Survival; D003470:Culture Media; D004273:DNA, Neoplasm / Q000096:biosynthesis; D005260:Female; D005947:Glucose / Q000378:metabolism; D006736:Horses; D006801:Humans; D007773:Lactates / Q000378:metabolism; D051379:Mice; D009363:Neoplasm Proteins / Q000096:biosynthesis; D012334:RNA, Neoplasm / Q000096:biosynthesis; D012709:Serum Albumin
D000596:Amino Acids; D000621:Aminoisobutyric Acids; D003470:Culture Media; D004273:DNA, Neoplasm; D007773:Lactates; D009363:Neoplasm Proteins; D012334:RNA, Neoplasm; D012709:Serum Albumin; D005947:Glucose