Growth of Mycoplasma synoviae in a medium supplemented with nicotinamide instead of B-nicotinamide adenine dinucleotide.
The cultivation of Mycoplasma synoviae (MS) on growth medium requires supplementation with B-nicotinamide adenine dinucleotide (NAD), an expensive and relatively unstable compound. Strains of MS were adapted (selected) to grow in a medium supplemented instead with nicotinamide, a stable and inexpensive substance that can be heat-sterilized in the medium without impairing its growth-promoting properties. MS plate agglutinating antigens grown in nicotinamide-enriched media were comparable in yield and serological reaction to antigens grown with NAD supplementation.
D000942:Antigens, Bacterial; D003470:Culture Media; D005285:Fermentation; D009174:Mycoplasma / Q000254:growth & development* / Q000276:immunology / Q000378:metabolism; D009243:NAD / Q000378:metabolism*; D009536:Niacinamide / Q000378:metabolism*
D000942:Antigens, Bacterial; D003470:Culture Media; D009243:NAD; D009536:Niacinamide