[Protamine as a specific inhibitor of electron transport between cytochromes c and a on the outer surface of the mitochondrial membrane].
The basic protein protamine is found to be a potent inhibitor of mitochondrial cytochrome c oxidase, while the oxidase activity of the "inside out" submitochondrial particles is only slightly affected by this polycation. The site of inhibition of mitochondrial respiration by protamine is localized between cytochromes c and a, protamine combining with cytochrome oxidase competitively to cytochrome, c, Ki=2,5 x 10(-6) M. The data obtained suggest that it is the outer side of the mitochondrial membrane where oxidation of cytochrome c by cytochrome a occurs.
D000818:Animals; D002417:Cattle; D003574:Cytochrome c Group / Q000378:metabolism; D003580:Cytochromes / Q000378:metabolism*; D004579:Electron Transport / Q000187:drug effects; D003576:Electron Transport Complex IV / Q000037:antagonists & inhibitors*; D008566:Membranes / Q000378:metabolism; D008931:Mitochondria, Muscle / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism; D010084:Oxidation-Reduction; D011479:Protamines / Q000494:pharmacology*
D003574:Cytochrome c Group; D003580:Cytochromes; D011479:Protamines; D003576:Electron Transport Complex IV
Protamin Kak Spetsifichesksii Ingibitor Perenosa Zlektronov Ot Tsitokhroma c K Tsitokhromu a Na Vneshchnei Poverkhnosti Membrany Mitokhondrii