Isolation of the plasma membrane from sea urchin embryos.
A method for isolation of sea urchin embryos plasma membranes is described. Purification of the obtained fraction was assayed by several enzymatic markers and electron microscopy. The isolated plasma membranes appear to be pure from contamination of other cell membranes (endoplasmic reticulum and mitochondria), and they can therefore be used for analytical studies on the composition and structure of plasma membrane.
D000818:Animals; D002458:Cell Fractionation / Q000379:methods*; D002462:Cell Membrane / Q000201:enzymology; D003576:Electron Transport Complex IV / Q000378:metabolism; D005260:Female; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D009708:Nucleotidases / Q000378:metabolism; D010063:Ovum / Q000648:ultrastructure; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D012617:Sea Urchins; D013347:Subcellular Fractions / Q000201:enzymology
D009247:NADH, NADPH Oxidoreductases; D003576:Electron Transport Complex IV; D009708:Nucleotidases; D010727:Phosphoric Diester Hydrolases