The use of 75Se-methionine as a tracer of thrombocytopoiesis. I. In vivo incorporation of the tracer into platelet proteins: a biochemical study.
This study was undertaken to define to which platelet components 75Se-methionine is bound after its injection to normal rats, and to study the curves of specific radioactivity of each labelled fraction. It has been shown that, for a part, platelet labelling is due to adsorption of plasma proteins (albumin and fibrinogen) after their synthesis. For another part, methionine is directly incorporated in the bone marrow precursors into platelet actomyosin, a protein constituent of the cells; the variations of the specific radioactivity of this component indicate that 75Se-methionine can be used as a cohort label of platelets.
D000205:Actomyosin / Q000302:isolation & purification / Q000378:metabolism; D000818:Animals; D001792:Blood Platelets / Q000378:metabolism*; D001798:Blood Proteins / Q000302:isolation & purification / Q000378:metabolism; D002850:Chromatography, Gel; D005340:Fibrinogen / Q000302:isolation & purification / Q000378:metabolism; D006025:Glycosaminoglycans / Q000097:blood / Q000302:isolation & purification; D006410:Hematopoiesis; D005779:Immunodiffusion; D008297:Male; D051381:Rats; D012643:Selenium; D012645:Selenomethionine / Q000097:blood; D012709:Serum Albumin / Q000302:isolation & purification / Q000378:metabolism
D001798:Blood Proteins; D006025:Glycosaminoglycans; D012709:Serum Albumin; D005340:Fibrinogen; D000205:Actomyosin; D012645:Selenomethionine; D012643:Selenium