Demonstration and semiquantitative determination of complexes between various proteases and human alpha2-macroglobulin.
Complex formation in vitro between human alpha2-macroglobulin and the human proteases cationic trypsin, chymotrypsin, plasmin and granulocyte elastase and collagenase was clearly visualized by the use of thin-layer electrofocusing in polyacrylamide gel followed by electrophoresis in agarose gel containing antibodies against human alpha2-macroglobulin. The technique permits semi-quantitative determination of the amount of complex and can demonstrate the formation of complexes between alpha2-macroglobulin and protease in vivo in ascitic fluid in peritonitis.
D001665:Binding Sites; D002918:Chymotrypsin / Q000097:blood; D005341:Fibrinolysin / Q000378:metabolism; D006098:Granulocytes / Q000201:enzymology; D006801:Humans; D007122:Immunoelectrophoresis; D008259:Macroglobulins / Q000378:metabolism*; D046911:Macromolecular Substances; D003012:Microbial Collagenase / Q000097:blood; D010196:Pancreatic Elastase / Q000097:blood; D010447:Peptide Hydrolases / Q000032:analysis / Q000097:blood*; D011485:Protein Binding; D014357:Trypsin / Q000097:blood
D008259:Macroglobulins; D046911:Macromolecular Substances; D010447:Peptide Hydrolases; D002918:Chymotrypsin; D010196:Pancreatic Elastase; D014357:Trypsin; D005341:Fibrinolysin; D003012:Microbial Collagenase