title: Biochemical changes after hepatic injury from toxic doses of acetaminophen or furosemide.
abstract: The effects of hepatotoxic doses of acetaminophen and furosemide on the function and composition of hepatic endoplasmic reticulum were compared from 3 to 24 h after administration. Acetaminophen caused a significant decrease in microsomal protein concentration as early as 3 h after its administration, but furosemide did not affect the microsomal protein concentration until 24 h after the dose. Both acetaminophen and furosemide decreased the concentrations of cytochrome P-450 and cytochrome b5 in microsomes, and the activity of microsomal ethylmorphine N-demethylase and aniline hydroxylase. Glucose-6-phosphatase and UDP-glucuronyl transferase were not significantly affected by acetaminophen or furosemide administration, and neither diene conjugation nor hepatic triglycerides were increased. Incorporation of 3H-L-leucine into liver proteins was decreased by 50% after the administration of either acetaminophen or furosemide.
mesh_terms: D000082:Acetaminophen / Q000506:poisoning*; D000815:Aniline Hydroxylase / Q000378:metabolism; D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000209:etiology / Q000378:metabolism*; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000201:enzymology / Q000378:metabolism; D005037:Ethylmorphine-N-Demethylase / Q000378:metabolism; D005665:Furosemide / Q000506:poisoning*; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D014453:Glucuronosyltransferase / Q000378:metabolism; D008099:Liver / Q000187:drug effects*; D008297:Male; D051379:Mice; D008862:Microsomes, Liver / Q000201:enzymology / Q000378:metabolism*; D011506:Proteins / Q000378:metabolism
chemical_list: D011506:Proteins; D000082:Acetaminophen; D005665:Furosemide; D003577:Cytochrome P-450 Enzyme System; D000815:Aniline Hydroxylase; D005037:Ethylmorphine-N-Demethylase; D014453:Glucuronosyltransferase; D005952:Glucose-6-Phosphatase