title: Lymphoproliferative disease in a cotton-top marmoset after inoculation with infectious mononucleosis-derived Epstein-Barr virus.
abstract: Injection of concentrated EBV derived from cells of the Kaplan line of infectious mononucleosis (IM) origin resulted in malignant lymphoproliferation in one out of three cotton-top marmosets 6 weeks after inoculation. Two additional animals receiving the same isolate after incubation with an antibody-containing human serum did not develop tumors. Inoculation of concentrated virus derived from the P3HR-1 line of Burkitt origin did not lead to lymphoproliferations in five marmosets. Three of these received non-neutralized, and two received neutralized P3HR-1 virus. The tumor obtained with the Kaplan isolate revealed characteristics of a lymphosarcome. It contained EBV-specific DNA. In addition, EBV-synthesizing lymphoblastoid lines were established from a tumorous lymph-node, as well as from the spleen of the diseased marmoset. Virus recovered from these lines transformed lymphocytes derived from spleens of healthy marmosets. The tumor-bearing animal developed low levels of anti-VCA antibodies during the course of tumor growth. These data demonstrate the oncogenic potential of EBV directly derived from cells of IM origin.
mesh_terms: D000818:Animals; D000906:Antibodies; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis; D000914:Antibodies, Viral / Q000032:analysis; D002051:Burkitt Lymphoma / Q000382:microbiology; D002143:Callitrichinae; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D004279:DNA, Viral / Q000302:isolation & purification; D005260:Female; D004854:Herpesvirus 4, Human / Q000276:immunology / Q000302:isolation & purification; D007244:Infectious Mononucleosis / Q000382:microbiology*; D008198:Lymph Nodes / Q000382:microbiology; D008223:Lymphoma / Q000209:etiology* / Q000473:pathology; D008228:Lymphoma, Non-Hodgkin / Q000276:immunology / Q000473:pathology; D008297:Male; D009374:Neoplasms, Experimental / Q000209:etiology / Q000473:pathology; D013154:Spleen / Q000382:microbiology; D014779:Virus Replication
chemical_list: D000906:Antibodies; D000888:Antibodies, Anti-Idiotypic; D000914:Antibodies, Viral; D004279:DNA, Viral