Species restriction of the mitogenicity induced by lanatoside C. Lymphocyte activation by digitalis glycosides is confined to cells from digitalis resistant species.
Activation of Na+, K+-ATPase has previously been suggested to be the triggering signal in mitogen-induced cell activation. Using a digitalis glycoside known to be a potent polyclonal B-cell activator, this hypothesis could be tested since digitalis activates ATPase at different concentrations in various species, depending on the degree of sensitivity to the toxic effects of glycosides. Lanatoside C was found to stimulate lymphocytes from glycoside resistant species such as rat, mouse and hamster. The possible involvement of Na+, K+-ATPase was made less likely by the similarity in dose--response profile in these cells although they have been reported to display varying degrees of glycoside resistance. Furthermore, using lymphocytes from digitalis-sensitive species such as man, guinea-pig or rabbit, no mitogenicity could be recorded, strongly suggesting a lack of correlation between glycoside-induced effects on Na+, K+-ATPase and cell activation.
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000328:Adult; D000818:Animals; D002478:Cells, Cultured; D002648:Child; D006224:Cricetinae; D004247:DNA / Q000096:biosynthesis; D004305:Dose-Response Relationship, Drug; D006168:Guinea Pigs; D006801:Humans; D007799:Lanatosides / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000166:cytology / Q000378:metabolism; D051379:Mice; D008934:Mitogens / Q000494:pharmacology; D008938:Mitosis / Q000187:drug effects*; D011817:Rabbits; D051381:Rats; D013045:Species Specificity
D007799:Lanatosides; D008934:Mitogens; D004247:DNA; D000251:Adenosine Triphosphatases