Simian virus 40 DNA replication, transcription, and antigen induction during infection with two adenovirus 2-SV40 hybrids that contain the entire SV40 genome.
The Ad2++hey hybrid virus population produces simian virus 40 (SV40) efficiently during lytic infection, whereas Ad2++ley does not, although both hybrids contain a complete SV40 genome. In this report, we demonstrate the synthesis of nonhydrid SV40 DNA in Ad2++HEY-infected Vero cells, but only early SV40 RNA is transcribed efficiently in Ad2++LEY-infected cells. Ad2++HEY induces SV40 U, T, and V antigens during lytic infection of African green monkey kidney cells, whereas Ad2++LEY induces only SV40 U and T antigens. These variations in the behavior of Ad2++HEY and Ad2++LEY regarding expression of SV40 functions probably reflect differences in the rate of SV40 excision from the hybrid genomes.
D000256:Adenoviridae; D000818:Animals; D000956:Antigens, Viral; D002460:Cell Line; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis*; D014644:Genetic Variation; D000882:Haplorhini; D006801:Humans; D006824:Hybridization, Genetic; D007668:Kidney / Q000196:embryology; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000096:biosynthesis; D013539:Simian virus 40 / Q000276:immunology / Q000378:metabolism*; D014158:Transcription, Genetic
D000956:Antigens, Viral; D004279:DNA, Viral; D012367:RNA, Viral