title: A genetic recombination map of foot-and-mouth disease virus.
abstract: Sixty ts mutants were isolated from the Pacheco strain of type O foot-and-mouth disease virus after treatment with either 5-fluorouracil or hydroxylamine. The conditions affecting recombination and assay of the ts+ recombinants were standardized. Using two ts mutants resistant to guanidine, three-factor crosses, supported by two-factor crosses, located 34 of the mutations in a linear arrangement. The recombination frequencies between certain pairs of mutations were additive. The guanidine character of the two resistant mutants mapped as a single site mutation and was located near the middle of the map.
mesh_terms: D000818:Animals; D005537:Aphthovirus / Q000187:drug effects / Q000254:growth & development; D002460:Cell Line; D002874:Chromosome Mapping; D006224:Cricetinae; D003433:Crosses, Genetic; D004352:Drug Resistance, Microbial; D005472:Fluorouracil; D005796:Genes; D006146:Guanidines / Q000494:pharmacology; D006898:Hydroxylamines; D007668:Kidney; D009153:Mutagens; D009154:Mutation; D011995:Recombination, Genetic; D013696:Temperature; D010948:Viral Plaque Assay
chemical_list: D006146:Guanidines; D006898:Hydroxylamines; D009153:Mutagens; D005472:Fluorouracil