Biogenesis of intranuclear lead-protein inclusions in mouse kidney.
A single dose of lead (5 mug/g body weight), given as lead acetate by intracardiac injection, produces, within 8 hours, characteristic fibrillar intranuclear and intracytoplasmic inclusions in proximal tubular epithelial cells in mouse kidneys; after a dose of 40 mug/g body weight, such inclusion appeared within 6 hours. Their development was completely prevented by cycloheximide (one or more intraperitoneal injections of 20 mug/g body weight). The development of intranuclear inclusions was also noted in tubular epithelial cells explanted from normal mice and grown in vitro for 15 hours in a medium containing 20 mug lead/ml. Thus, the development of the characteristic fibrillar inclusion bodies depends upon de novo synthesis of inclusion body protein, induced by lead.
D000818:Animals; D002467:Cell Nucleus / Q000032:analysis; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D003593:Cytoplasm / Q000648:ultrastructure; D004847:Epithelial Cells; D004848:Epithelium / Q000032:analysis; D005260:Female; D002479:Inclusion Bodies / Q000032:analysis; D007687:Kidney Tubules, Proximal / Q000648:ultrastructure*; D007854:Lead / Q000032:analysis / Q000494:pharmacology; D007855:Lead Poisoning / Q000473:pathology*; D014176:Protein Biosynthesis; D011506:Proteins / Q000032:analysis; D051381:Rats; D013997:Time Factors
D011506:Proteins; D007854:Lead; D003513:Cycloheximide