Binding of Concanavalin A and its effect on the differentiation of Dictyostelium Discoideum.
Dictyostelium discoideum amoebae have two classes of binding sites for concanavalin A, one accessible to a competitive inhibitor of concanavalin A binding and one inaccessible. Uptake of concanavalin A occurs and is associated with a transfer from accessible to inaccessible binding sites. Low amounts of concanavalin A bound to cells produce a slight stimulation of differentiation, and this is associated with an increase in production of phosphodiesterase. Above a critical amount of concanavalin A bound, cell differentiation and phosphodiesterase production are inhibited. The relationship of concanavalin A-induced membrane changes to cell differentation is discussed.
D000118:Acetylglucosaminidase / Q000032:analysis; D001665:Binding Sites / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D003208:Concanavalin A / Q000378:metabolism*; D004023:Dictyostelium / Q000378:metabolism*; D007700:Kinetics; D037102:Lectins / Q000494:pharmacology; D009235:Myxomycetes / Q000378:metabolism*; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism
D037102:Lectins; D003208:Concanavalin A; D010727:Phosphoric Diester Hydrolases; D000118:Acetylglucosaminidase