Cyanobacterial ribonucleic acid polymerases recognize lambda promoters.
We compared the initiation specificities in vitro of deoxyribonucleic acid-dependent ribonucleic acid polymerases purified from two cyanobacteria, Fremyella diplosiphon and Anacystis nidulans, and from Escherichia coli. A restriction fragment made from lambda deoxyribonucleic acid was used as a template. The cyanobacterial and E. coli ribonucleic acid polymerases recognized the same lambda promoters but exhibited different sensitivities to the inhibitor heparin, suggsesting differences in the structure of the initiation complexes.
D010582:Bacteriophage lambda; D000458:Cyanobacteria / Q000201:enzymology*; D004269:DNA, Bacterial / Q000235:genetics*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D006493:Heparin / Q000494:pharmacology; D009876:Operon; D012097:Repressor Proteins / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects; D014764:Viral Proteins / Q000494:pharmacology
D004269:DNA, Bacterial; D012097:Repressor Proteins; D014764:Viral Proteins; D006493:Heparin; D012321:DNA-Directed RNA Polymerases