title: Role of carbohydrate of human chorionic gonadotropin in the mechanism of hormone action.
abstract: The role of the carbohydrate part of human chorionic gonadotropin (hCG) was investigated by measuring the ability of hCG derivatives lacking various sugar residues to bind to rat Leydig cells and stimulate them to synthesize testosterone and cyclic adenosine 3':5'-monophosphate (cyclic AMP). Whereas sequential removal of the sialic acid, galactose, N-acetylglucosamine, and mannose residues led to a progressive increase in the effective dose of the hormone required to stimulate steroidogenesis, it resulted in a marked loss in the ability of the hormone to stimulate cyclic AMP accumulation. Low doses of the glycosidase-treated hormone derivatives were additive with hCG when their ability to stimulate testosterone synthesis was analyzed. Nevertheless, the glycosidase-treated derivatives were potent inhibitors of hCG-induced cyclic AMP accumulation, suggesting that removal of the sugars did not influence binding of the hormone to the cell as much as it reduced the ability of the bound hormone to activate adenyl cyclase. This hypothesis was further supported by our finding that the hCG derivatives were highly effective inhibitors of 125I-hGC binding to the intact cells. Removal of sialic acid and galactose enhanced the inhibition, whereas removal of all the sugar residues only decreased the inhibition slightly. The degree of these effects was comparatively small. The possibility that steroidogenesis and cyclic AMP accumulation are altered independently by hCG stimulation is discussed.
mesh_terms: D000118:Acetylglucosaminidase; D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D006063:Chorionic Gonadotropin / Q000032:analysis / Q000378:metabolism* / Q000494:pharmacology; D005696:Galactosidases; D007985:Leydig Cells / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008361:Mannosidases; D009439:Neuraminidase; D051381:Rats; D011956:Receptors, Cell Surface; D013739:Testosterone / Q000096:biosynthesis
chemical_list: D002241:Carbohydrates; D006063:Chorionic Gonadotropin; D011956:Receptors, Cell Surface; D013739:Testosterone; D005696:Galactosidases; D008361:Mannosidases; D009439:Neuraminidase; D000118:Acetylglucosaminidase