Inhibition of lymphocyte transformation: effect of soy bean trypsin inhibitor and synthetic anti-proteases.
Soybean trypsin inhibitor (SBI) was found to inhibit transformation of human lymphocytes induced by mitogens (leucoagglutinin, concanavalin A, NaIO4) or in mixed lymphocyte reaction (MLR). SBI covalently cross-linked to Sepharose beads inhibited the MLR and mitogen stimulation virtually completely. We have confirmed the work of others which showed that the synthetic anti-proteases epilson-aminocaproic acid and tosyl-L-lysyl-chloromethane (TLCK) also inhibited mitogen-induced blastogenesis and we have shown that phenylmethylsulfonylfluride was effective also; all of these agents were found to inhibit the MLR as well. SBI and TLCK were most inhibitory when added along with mitogen or when mixing allogeneic cells in a MLR; significant decrease in inhibition was noted when TLCK was added 1 h after mitogen. These data support the hypothesis that protease action at a cell surface is an essential early event common to all types of lymphocyte transformation.
D000373:Agglutinins / Q000494:pharmacology; D001665:Binding Sites; D003208:Concanavalin A / Q000494:pharmacology; D006801:Humans; D010504:Periodic Acid / Q000494:pharmacology; D011485:Protein Binding; D012685:Sepharose; D014360:Trypsin Inhibitor, Kunitz Soybean / Q000494:pharmacology*; D014361:Trypsin Inhibitors / Q000494:pharmacology*
D000373:Agglutinins; D014361:Trypsin Inhibitors; D010504:Periodic Acid; D003208:Concanavalin A; D012685:Sepharose; D014360:Trypsin Inhibitor, Kunitz Soybean