Host-dependent restriction of mengovirus replication. II. Effect of host restriction on late viral RNA synthesis and viral maturation.
Restricted mengovirus replication in Mandin-Darby bovine kidney (MDBK) cells is characterized by a 400-fold reduction in infectious virus yield and a 40-fold increase in the production of noninfectious virus. Using conditions which insure that all MDBK cells are infected, virus-specific RNA and protein synthesis were measured in the restrictive host and in a permissive host for mengovirus, HeLa cells. Labeling kinetics and sucrose gradient analysis of mengovirus-specific RNA from MDBK cells show a reduction of 10-fold in virion RNA, 5-fold in double-stranded RNA, and 12.5-fold in single-stranded RNA. The viral RNA biosynthetic processes which occur late in the replicative cycle and result in the production of 90% of the single-stranded viral RNA that is packaged into capsid proteins in the permissive host are absent in restrictive MDBK cells. Viral protein synthesis as measured by labeled viral-specific polysome is decreased, and there is an accumulation of 80S subviral particles in the restricted host. It is suggested that restriction may act at a number of stages of viral replication and maturation.
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002250:Carbon Radioisotopes; D002417:Cattle; D002460:Cell Line; D002501:Centrifugation, Zonal; D004591:Electrophoresis, Polyacrylamide Gel; D006367:HeLa Cells; D007668:Kidney; D007739:L Cells; D008574:Mengovirus / Q000254:growth & development*; D011492:Protein Hydrolysates; D012367:RNA, Viral / Q000096:biosynthesis*; D014316:Tritium; D014529:Uridine; D014779:Virus Replication
D000596:Amino Acids; D002250:Carbon Radioisotopes; D011492:Protein Hydrolysates; D012367:RNA, Viral; D014316:Tritium; D014529:Uridine