Procollagenase from bovine gingiva.
1. Collagenase (EC 3.4.24.3) is released from bovine gingival explants in vitro as a zymogen. The zymogen does not hydrolyze collagen and does not form a complex with alpha2-macroglobulin (alpha2-M). It elutes in gel filtration with an apparent molecular weight of approx. 80 000. 2. Incubation of the zymogen with trypsin results in a 15 000-20 000 dalton decrease in molecular weight and imparts to the enzyme the ability to hydrolyze collagen and to form a complex with alppha2-M. 3. The zymogen can be completely separated from the active enzyme to alpha2-M. Likewise, the zymogen can be harvested from cultures supplemented with serum.
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D004789:Enzyme Activation / Q000187:drug effects; D004792:Enzyme Precursors / Q000378:metabolism*; D004950:Esterases / Q000378:metabolism; D005881:Gingiva / Q000201:enzymology*; D007700:Kinetics; D003012:Microbial Collagenase / Q000378:metabolism*; D014357:Trypsin / Q000494:pharmacology
D004792:Enzyme Precursors; D004950:Esterases; D014357:Trypsin; D003012:Microbial Collagenase