Detection of virus-specific immunoglobulins using a doubly labeled fluorescein-125I antibody.
Commercially prepared fluorescein-labeled antihuman antibodies were labed with 125I and used to compre specific herpes simplex virus antibody titers as determined by indirect fluorescent antibody and radioimmunoassay techniques. Total virus-specific immunoglobulin and virus-specific immunoglobulin G titers did not vary by more than one twofold dilution when compared by the two methods.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D000914:Antibodies, Viral / Q000032:analysis*; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis*; D007457:Iodine Radioisotopes; D011863:Radioimmunoassay; D012756:Sheep / Q000276:immunology; D018139:Simplexvirus / Q000276:immunology*
D000888:Antibodies, Anti-Idiotypic; D000914:Antibodies, Viral; D005452:Fluoresceins; D007136:Immunoglobulins; D007457:Iodine Radioisotopes