Nitrosyl-hemoglobin formation in the blood of animals exposed to nitric oxide.
Animals were exposed to nitric oxide (NO) in order to study its biological effect. First a method was developed to exclude nitrogen dioxide formed during exposure of NO. Nitrosyl-hemoglobin (NOHb) in the blood of mice, rats, and rabbits exposed to NO was determined by electron spin resonance spectrometry at -100C. The content of NOHb in the blood of the mouse reached the maximum (0.13% of the total hemoglobin) 20 minutes after the exposure was begun and thereafter a state of equilibrium was maintained. The NOHv level decreased to one half in several minutes after the mouse was removed to a filtered air environment. Dose-response relationship was observed between gas concentrations and the maximum NOHb level.
D000818:Animals; D001273:Atmosphere Exposure Chambers; D004305:Dose-Response Relationship, Drug; D004578:Electron Spin Resonance Spectroscopy; D004781:Environmental Exposure; D005260:Female; D006454:Hemoglobins / Q000032:analysis*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008815:Mice, Inbred Strains; D009569:Nitric Oxide / Q000633:toxicity*; D009603:Nitroso Compounds / Q000097:blood; D011817:Rabbits; D051381:Rats
D006454:Hemoglobins; D009603:Nitroso Compounds; D009569:Nitric Oxide