title: Changes in membrane structure and function during chick embryonic erythropoiesis.
abstract: We found considerable differences in the pattern of membrane proteins as well as in the relative amounts of individual components in isolated chick red blood cell membranes during the course of embryonic development. Of special interest in the increase in the relative amounts of two major polypeptides, band 3 and 3.1 (MW 100,000 daltons) with increasing age of the cells. With respect to functional studies, we found that the magnitude of sulfate influx decreases with increasing age of the embryo. Furthermore, the activity of ouabain-sensitive ATPase increases with increasing age (2.5-day embryo to adult). In addition, both the basal and the fluoride-stimulated adenylate cyclase activities decrease as the embryo age increases, whereas the enzyme sensitivity to epinephrine increases with increasing age of the embryo.
mesh_terms: D000251:Adenosine Triphosphatases / Q000097:blood; D000262:Adenylyl Cyclases / Q000097:blood; D000818:Animals; D001692:Biological Transport; D002642:Chick Embryo; D004910:Erythrocyte Membrane / Q000502:physiology*; D004912:Erythrocytes / Q000502:physiology*; D004920:Erythropoiesis; D006801:Humans; D007444:Inulin / Q000097:blood; D008565:Membrane Proteins / Q000097:blood*; D008970:Molecular Weight; D010042:Ouabain / Q000494:pharmacology; D013045:Species Specificity
chemical_list: D008565:Membrane Proteins; D010042:Ouabain; D007444:Inulin; D000251:Adenosine Triphosphatases; D000262:Adenylyl Cyclases