Assembly of the mitochondrial membrane system. Cytoplasmic mutants of Saccharomyces cerevisiae with lesions in enzymes of the respiratory chain and in the mitochondrial ATPase.
Mutants of Saccharomyces cervisiae with defects in enzymes of the electron transfer chain and in the rutamycin-sensitive ATPase have been isolated. Some of the mutants are specifically affected in either cytochrome oxidase, coenzyme QH2-cytochrome c reductase or ATPase. Other strains are deficient in both cytochrome oxidase and coenzyme QH2-cytochrome c reductase but still have rutamycin-sensitive ATPase. All the mutants reported in this study fail to be complemented by a rho0 tester derived from a respiratory competent strain. The meiotic spore progeny obtained by mating the mutants to a respiratory competent haploid yeast, when scored for growth on glycerol, show a non-Mendelian segregation of the phenotype. These two genetic tests indicate the mutations to be cytoplasmically inherited.
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D003579:Cytochrome Reductases / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000378:metabolism*; D005816:Genetic Complementation Test; D008566:Membranes / Q000187:drug effects / Q000201:enzymology; D008928:Mitochondria / Q000201:enzymology*; D009154:Mutation; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D010641:Phenotype; D012427:Rutamycin / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D013172:Spores, Fungal / Q000378:metabolism; D014451:Ubiquinone
D014451:Ubiquinone; D012427:Rutamycin; D003579:Cytochrome Reductases; D009251:NADPH-Ferrihemoprotein Reductase; D003576:Electron Transport Complex IV; D000251:Adenosine Triphosphatases