Effects of ethyl p-chlorophenoxyisobutyrate on carbohydrate and fatty acid metabolism in rat liver.
1. Rats were fed on a diet containing ethyl p-chlorophenoxyisobutyrate (0.3%, w/w) for 14 days. 2. The alterations of contents of intermediates in the liver indicate that gluconeogenesis is inhibited at the reaction(s) between 3-phosphoglycerate and fructose 1,6-diphosphate. The [nad+]/([nadh] ratios in cytoplasm and mitochondria were increased about 3- and 4-fold, respectively. Marked increases in the contents of CoA and its thioesters were found. 3. Hepatic fatty acid synthesis increased about 3-fold. There was no evidence of inhibition of the acetyl-CoA carboxylase [EC 6.4.1.2] reaction by the drug.
D000818:Animals; D001835:Body Weight; D050260:Carbohydrate Metabolism; D002784:Cholesterol / Q000097:blood; D002994:Clofibrate / Q000494:pharmacology*; D003593:Cytoplasm / Q000378:metabolism; D004032:Diet; D005227:Fatty Acids / Q000378:metabolism*; D005636:Fructosephosphates / Q000378:metabolism; D005943:Gluconeogenesis / Q000187:drug effects*; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D005994:Glycerophosphates / Q000378:metabolism; D006598:Hexosediphosphates / Q000378:metabolism; D006884:Hydroxybutyrate Dehydrogenase / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D009929:Organ Size; D051381:Rats; D014280:Triglycerides / Q000097:blood
D005227:Fatty Acids; D005636:Fructosephosphates; D005994:Glycerophosphates; D006598:Hexosediphosphates; D014280:Triglycerides; D009243:NAD; D002784:Cholesterol; D007770:L-Lactate Dehydrogenase; D006884:Hydroxybutyrate Dehydrogenase; D005969:Glutamate Dehydrogenase; D002994:Clofibrate