The interaction of NADH-cytochrome b5 reductase and cytochrome b5 bound to egg lecithin liposomes.
Incubation of liposomes prepared by sonication of egg lecithin with the amphipathic form of cytochrome b5 results in the binding of a maximum of 244 molecules of cytochrome b5 per liposomal vesicle. Interactions of the phospholipid with the hydrophobic segment of cytochrome b5 are involved in this binding which does not disrupt the liposome. When a small amount of NADH-cytochrome b5 reductase is bound liposomes simultaneously with cytochrome b5, the two proteins catalyze the reduction of cytochrome c by NADH. A qualitative kinetic analysis reveals that all of the cytochrome b5 interacts with reductase, a result consistent with these protein undergoing translational diffusion in the plane of the membrane. This system and the purified stearyl coenzyme A desaturase provide a model to study the dynamics of protein andlipid interactions in this membrane-bound oxidative sequence.
D000818:Animals; D002250:Carbon Radioisotopes; D002417:Cattle; D002708:Chlorella; D003579:Cytochrome Reductases / Q000378:metabolism*; D003580:Cytochromes / Q000378:metabolism*; D003903:Deuterium; D004531:Eggs; D007700:Kinetics; D008081:Liposomes / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008297:Male; D008854:Microscopy, Electron; D009243:NAD / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D011485:Protein Binding
D002250:Carbon Radioisotopes; D003580:Cytochromes; D008081:Liposomes; D010713:Phosphatidylcholines; D010743:Phospholipids; D009243:NAD; D003903:Deuterium; D003579:Cytochrome Reductases