17Beta-hydroxysteroid dehydrogenase activity in Streptomyces hydrogenans.
Streptomyces hydrogenans converts 17beta-hydroxyandrost-4-ene-3-one (testosterone) to androst-4-ene-3,17-dione (androstenedione) in good yields. Time-dependence of the conversion, steroid uptake and release have been studied in vivo. Steroid analysis was done by thin-layer chromatography and recrystallization to constant specific radioactivity. After sonification of the cells the postulated 17beta-hydroxysteroid dehydrogenase activity was recovered in the 105 000 g supernatant. The enzyme was enriched by gel filtration on Sephadex G-200. It required NAD+ as cofactor. Its activity could be studied photometrically, because there are no further testosterone-netabolites. If S. hydrogenans was cultured in the presence of testosterone, estradiol or 5alphaH-dihydrotestosterone, the activity of 17beta-hydroxysteroid dehydrogenase increased.
D000735:Androstenedione / Q000096:biosynthesis; D002474:Cell-Free System; D003600:Cytosol / Q000378:metabolism; D013196:Dihydrotestosterone / Q000494:pharmacology; D004958:Estradiol / Q000494:pharmacology; D006913:Hydroxysteroid Dehydrogenases / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D011284:Pregnenolone / Q000494:pharmacology; D012270:Ribosomes / Q000378:metabolism; D013302:Streptomyces / Q000201:enzymology*; D013347:Subcellular Fractions / Q000378:metabolism; D013739:Testosterone / Q000378:metabolism / Q000494:pharmacology
D013196:Dihydrotestosterone; D009243:NAD; D013739:Testosterone; D000735:Androstenedione; D004958:Estradiol; D011284:Pregnenolone; D006913:Hydroxysteroid Dehydrogenases