title: Phosphoprotein phosphatase activity in the thyroid.
abstract: Phosphoprotein phosphatase activity in the calf thyroid was found in various subcellular fractions. The relative amount in each fraction varied according to the substrate used: The 500g fraction had the highest specific activity when protamine was used, while the 5000g fraction was highest when histone was used. Triton X-100 tended to increase activity in all the particulate fractions, the greatest change being found in the 105,000g pellet. DEAE chromatography of the 105,000 g supernatant resolved at least three peaks of phosphoprotein phosphatase activity.
mesh_terms: D000818:Animals; D002417:Cattle; D002458:Cell Fractionation; D002848:Chromatography, DEAE-Cellulose; D006657:Histones / Q000378:metabolism; D008566:Membranes / Q000201:enzymology; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis / Q000378:metabolism*; D011479:Protamines / Q000378:metabolism; D013961:Thyroid Gland / Q000201:enzymology*
chemical_list: D006657:Histones; D011479:Protamines; D010744:Phosphoric Monoester Hydrolases