Anesthesia and the golgi apparatus: enzyme activity changes in a golgi-rich fraction of rat liver after a short ether anesthesia.
Activity changes of enzymes in isolated rat liver Golgi preparations at different times (1-48 h) after a short ether anesthesia are reported. Activity of galactosyl-transferase showed a slight gradual increase but thiamine pyrophosphatase decreased sharply, and after 24 h increased to above control level. Arylsulphatase-A remained largely unchanged, and B was significantly decreased. Acid phosphatase activity did remain at the control level, but alkali phosphatase showed a gradual and highly significant increase. Five other enzymes representing probable contaminations from other subcellular organelles, have also been assayed. Correlation is sought between the enzyme activity changes and some other metabolic effects of anesthesia.
D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000768:Anesthesia, General; D000818:Animals; D005019:Ethyl Ethers; D005690:Galactose; D006056:Golgi Apparatus / Q000201:enzymology*; D006602:Hexosyltransferases / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D008297:Male; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D051381:Rats; D013429:Sulfatases / Q000378:metabolism; D013835:Thiamine Pyrophosphate; D013997:Time Factors
D005019:Ethyl Ethers; D006602:Hexosyltransferases; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D010744:Phosphoric Monoester Hydrolases; D013429:Sulfatases; D013835:Thiamine Pyrophosphate; D005690:Galactose