Blocks in elongation and initiation of protein synthesis induced by interferon treatment in mouse L cells.
Synthesis of polypeptide chains coded by exogenous messenger RNAs is inhibited in cell-free extracts from interferon-treated mouse L cells, due to a "deficiency" in some specific tRNA species. A detailed analysis shows that polypeptide chain elongation is blocked and incomplete chains are formed. After a few minutes, however, initiation of new polypeptide chains is also blocked. Messenger RNA still binds to ribosomes but initiator Met-tRNA(FMET) binding is inhibited. The block in initiation appears to be secondary to the block in elongation.
D000818:Animals; D002499:Centrifugation, Density Gradient; D002999:Clone Cells; D004591:Electrophoresis, Polyacrylamide Gel; D006454:Hemoglobins / Q000096:biosynthesis; D007372:Interferons / Q000494:pharmacology*; D007700:Kinetics; D007739:L Cells / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D008574:Mengovirus; D051379:Mice; D009239:N-Formylmethionine; D010441:Peptide Chain Elongation, Translational / Q000187:drug effects*; D010442:Peptide Chain Initiation, Translational / Q000187:drug effects*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D012343:RNA, Transfer / Q000378:metabolism; D011817:Rabbits; D012270:Ribosomes / Q000378:metabolism; D012967:Sodium Dodecyl Sulfate
D006454:Hemoglobins; D012333:RNA, Messenger; D012967:Sodium Dodecyl Sulfate; D009239:N-Formylmethionine; D007372:Interferons; D012343:RNA, Transfer