Modification of surface membrane antigens by trypsin.
Cultured 3T3 and PY-3T3 mouse fibroblasts were subjected to mild treatment with twice crystallized trypsin and their immunogenicity tested in ICR/CD-1 mice. Surface antigenic modifications were observed using the foot pad swelling assay for detection of delayed hypersensitive responses. Mice immunized with trypsin-treated 3T3 cells showed cell-mediated immune reactions following challenge with either homologous antigen or untreated PY-3T3 fibroblasts. In contrast, treatment of the virus-transformed cells with trypsin decreased their antigenicity as determined by this in vivo assay.
D000818:Animals; D000941:Antigens; D002462:Cell Membrane / Q000276:immunology; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D005347:Fibroblasts / Q000276:immunology*; D006968:Hypersensitivity, Delayed; D007111:Immunity, Cellular; D051379:Mice; D008813:Mice, Inbred ICR; D011120:Polyomavirus; D014357:Trypsin / Q000494:pharmacology*
D000941:Antigens; D014357:Trypsin