Phosphotriesters in rat liver deoxyribonucleic acid after the administration of the carcinogen NN-dimethylnitrosamine in vivo.
After treatment with NN-di[14C]methylnitrosamine, samples of DNA were isolated from rat livers by a conventional phenol procedure and examined for the presence of phosphotriesters. A method of capable of detecting relatively small amounts of 14C-labelled phosphotriesters was developed and used to establish that these products account for 10-12% of the total methylation pattern found after treatment with this agent in vitro. The significance of the presence of phosphotriesters in DNA is discussed.
D000478:Alkylation; D000818:Animals; D004247:DNA / Q000032:analysis* / Q000378:metabolism; D004128:Dimethylnitrosamine / Q000378:metabolism / Q000494:pharmacology*; D004952:Esters; D006868:Hydrolysis; D008099:Liver / Q000187:drug effects*; D008297:Male; D000432:Methanol / Q000378:metabolism; D009602:Nitrosamines / Q000494:pharmacology*; D009943:Organophosphorus Compounds / Q000032:analysis*; D010710:Phosphates / Q000378:metabolism; D010727:Phosphoric Diester Hydrolases; D051381:Rats; D013696:Temperature
D004952:Esters; D009602:Nitrosamines; D009943:Organophosphorus Compounds; D010710:Phosphates; D004247:DNA; D010727:Phosphoric Diester Hydrolases; D004128:Dimethylnitrosamine; D000432:Methanol