A kinetic method for serum 5'-nucleotidase using stabilised glutamate dehydrogenase.
A modification of a kinetic determination of 5'-nucleotidase (EC 3.1.3.5) activity is described. Special attention has been paid to the stabilisation of glutamate dehydrogenase (EC 1.4.1.2) by L-leucine, optimal NADH concentration and the influence of endogeneous ammonia. The optimal concentrations of the other constituents of the reagent were checked with the optimal values given in the literature. Normal values were determined. The proposed method shows a good correlation with a colorimetric reference method.
D000641:Ammonia / Q000097:blood; D004355:Drug Stability; D005969:Glutamate Dehydrogenase; D006801:Humans; D007202:Indicators and Reagents; D007930:Leucine; D008722:Methods; D009243:NAD; D009708:Nucleotidases / Q000097:blood*
D007202:Indicators and Reagents; D009243:NAD; D000641:Ammonia; D005969:Glutamate Dehydrogenase; D009708:Nucleotidases; D007930:Leucine