title: Ultrastructural demonstration of NAD-pyrophosphorylase activity in mouse liver nuclei.
abstract: Ultrastructural demonstration of NAD-pyrophosphorylase activity (E.C.2.7.7.1) in isolated mouse liver nuclei was investigated with the use of an electronhistochemical procedure based on the precipitation of pyrophosphate ions with lead ions under conditions permitting simultaneous ATPase inhibition by formaldehyde/ethanol prefixation. In isolated mouse liver nuclei activity of NAD-pyrophosphorylase was found in nucleoli, in interchromatin granules, coiled bodies and strand-like structures in nucleoplasm.
mesh_terms: D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors; D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology* / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism; D000431:Ethanol; D005557:Formaldehyde; D006651:Histocytochemistry; D008099:Liver / Q000201:enzymology* / Q000648:ultrastructure; D051379:Mice; D008854:Microscopy, Electron; D009243:NAD; D009537:Nicotinamide Mononucleotide; D009713:Nucleotidyltransferases / Q000378:metabolism*
chemical_list: D002843:Chromatin; D009243:NAD; D009537:Nicotinamide Mononucleotide; D005557:Formaldehyde; D000431:Ethanol; D009713:Nucleotidyltransferases; D000251:Adenosine Triphosphatases