[Subcellular distribution of the protein kinase activity in the normal and neoplastic thyroid tissue of the rat].
Thyroid tissue has been fractionnated by centrifugation (105 000 q) of its homogenate. Protein-kinase activity in presence of histone is distributed in nuclei (11.5%) mitochondira (22.8%), microsomes (9.8%) and soluble fraction (56%); it is activated by cyclic AMP and GMP, mostly in soluble and nuclear fractions. Protein-kinase activity of total homogenate of neoplasic thyroid (strain 1-5G Wollman) in presence of histone is 3 times higher than in normal tissue and more activated by cAMP. In absence of histone, protein-kinase activity is the more important in mitochondrial and microsomal fractions of normal thyroid and in soluble and nuclear fraction of neoplastic tissue.
D000818:Animals; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000201:enzymology; D002498:Centrifugation; D000242:Cyclic AMP / Q000494:pharmacology; D006152:Cyclic GMP / Q000494:pharmacology; D003576:Electron Transport Complex IV / Q000032:analysis; D007700:Kinetics; D008861:Microsomes / Q000201:enzymology; D008928:Mitochondria / Q000201:enzymology; D011494:Protein Kinases / Q000032:analysis*; D051381:Rats; D013347:Subcellular Fractions / Q000201:enzymology; D013961:Thyroid Gland / Q000201:enzymology*; D013964:Thyroid Neoplasms / Q000201:enzymology*
D000242:Cyclic AMP; D003576:Electron Transport Complex IV; D011494:Protein Kinases; D006152:Cyclic GMP
Répartition subcellulaire de l'activité protéine-kinase dans le tissu thyrodien normal et néoplasique de rat