Phospholipid biosynthesis in sarcoplasmic reticulum membrane during development.
Biosynthesis of phosphatidic acid, phosphatidylcholine and phosphatidylethanolamine in the sarcoplasmic reticulum membrane has been  investigated. The results show that sarcoplasmic reticulum, in addition to its main function, i.e. transport and accumulation of Ca2+, is able to synthetize phospholipids by the same pathways as endoplasmic reticulum of other tissues. The changes of activity of enzymes involved in phospholipid biosynthesis during muscle development have been analysed. The extent of sn-glycero-3-phosphate and lysophosphatidylcholine acylation by acyl-CoA or free fatty acids in the presence of ATP and CoA is the same at every stage of development. The specific activity of glycerolphosphate acyltransferase(s) increases progressively during development up to about the 10th day of postnatal life and then decreases to the adult level. Linoleate esterifies sn-glycero-3-phosphate to a higher extent than palmitate, especially during postnatal period. The main product of sn-glycero-3-phosphate acylation is phosphatidic acid. The specific activity of lysolecithin acyltransferase increases from the embryonic period to a maximum between the 4th and the 9th day of postnatal life followed by a decrease to the adult value. the low embryonic value to a maximum at about the 3rd day of postnatal life, followed by a decrease to the adult value. The activity of cholinephosphotransferase decreases from a high value observed during the earliest embryonic period studied until the 3rd day before birth, and then begins to increase again from about the 5th day of postnatal life. The activity of ethanolaminephosphotransferase decreases continuously with age. The main product of phosphatidylethanolamine methylation is phosphatidylmonomethylethanolamine. The specific activity of phosphatidylethanolamine methyltransferase increases from
D008243:1-Acylglycerophosphocholine O-Acyltransferase / Q000378:metabolism; D000375:Aging; D000818:Animals; D002798:Diacylglycerol Cholinephosphotransferase / Q000378:metabolism; D004622:Embryo, Mammalian; D005992:Glycerol-3-Phosphate O-Acyltransferase / Q000378:metabolism; D008566:Membranes / Q000378:metabolism; D008780:Methyltransferases / Q000378:metabolism; D008861:Microsomes / Q000378:metabolism; D024510:Muscle Development; D009132:Muscles / Q000378:metabolism; D010743:Phospholipids / Q000096:biosynthesis*; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000378:metabolism*
D010743:Phospholipids; D008780:Methyltransferases; D005992:Glycerol-3-Phosphate O-Acyltransferase; D008243:1-Acylglycerophosphocholine O-Acyltransferase; D002798:Diacylglycerol Cholinephosphotransferase