title: Thyroid hormone stimulates synthesis of a cardiac myosin isozyme. Comparison of the two-two-dimensional electrophoretic patterns of the cyanogen bromide peptides of cardiac myosin heavy chains from euthyroid and thyrotoxic rabbits.
abstract: The CNBr peptides of [14C]carboxymethylated cardiac myosin heavy chains from euthyroid and thyrotoxic rabbits have been compared using a two-dimensional electrophoretic system. The results indicated that there were extensive differences in the peptide "maps" of these heavy chains, which included differences in the distribution of radiolabeled thiol peptides. Also, the patterns of heavy chain peptides from the cardiac myosins have been compared with those produced by the heavy chain myosin isozymes from skeletal muscles. Peptide maps of heavy chains from red skeletal muscle myosin closely resembled the pattern of peptides found with cardiac myosin heavy chains from euthyroid rabbits. However, peptide maps of heavy chains from white skeletal muscle myosin were dissimilar to those of the cardiac myosin isozymes. We conclude that thyroxine administration stimulates the synthesis of a cardiac myosin isozyme with a heavy chain primary structure which is different from either of the skeletal muscle myosin isozymes.
mesh_terms: D000251:Adenosine Triphosphatases / Q000096:biosynthesis* / Q000302:isolation & purification; D000818:Animals; D003488:Cyanogen Bromide; D004591:Electrophoresis, Polyacrylamide Gel; D007527:Isoenzymes / Q000096:biosynthesis* / Q000302:isolation & purification; D009206:Myocardium / Q000201:enzymology*; D009218:Myosins / Q000096:biosynthesis*; D010446:Peptide Fragments / Q000302:isolation & purification; D011817:Rabbits; D013974:Thyroxine / Q000494:pharmacology*
chemical_list: D007527:Isoenzymes; D010446:Peptide Fragments; D000251:Adenosine Triphosphatases; D009218:Myosins; D003488:Cyanogen Bromide; D013974:Thyroxine