Splenocyte plaque assay for the detection of murine leukemia virus.
A modified XC assay for murine leukemia virus (MuLV) employing splenocytes taken directly from the animal is described. This modification can be more than 1000 times more sensitive than XC plaque assays employing tissue extracts. This technique should lend itself readily to the quantitation of infectious MuLV in defined populations of lymphoid cells.
D000818:Animals; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D009052:Leukemia Virus, Murine / Q000302:isolation & purification*; D008722:Methods; D051379:Mice; D008806:Mice, Inbred AKR; D008811:Mice, Inbred DBA; D013154:Spleen / Q000382:microbiology*; D013623:Tail; D014020:Tissue Extracts; D010948:Viral Plaque Assay
D014020:Tissue Extracts