Translation of Rous sarcoma virus RNA in a cell-free system from ascites Krebs II cells.
The template activities of the 60-70S RNA complex and of the 30-40S subunit RNA species of Rous sarcoma virus were tested in a cell-free protein-synthesizing system from mouse ascites Krebs II cells. Stimulation of protein synthesis over the endogenous background was about 2-fold with 30-40S viral RNA and about 1.3-fold with 60-70S viral RNA as template. Analysis by sodium dodecyl sulfate-gel electrophoresis showed that the predominant polypeptide synthesized in vitro in response to 30-40S RNA of Rous sarcoma virus had a molecular weight of 75,000-80,000. This polypeptide could be precipitated by antiserum against the group-specific antigens of the virus, although its molecular weight is higher than that of virion group-specific antigen proteins. Analysis of tryptic digests of the protein made in vitro indicates similarity to tryptic digests from authentic virion group-specific proteins. It is concluded that part of the RNA from Rous sarcoma virus is translated in vitro into a high-molecular-weight protein, perhaps a precursor of the virion group-specific proteins.
D000818:Animals; D001345:Autoradiography; D001358:Avian Sarcoma Viruses; D002287:Carcinoma, Krebs 2; D002474:Cell-Free System; D004591:Electrophoresis, Polyacrylamide Gel; D007118:Immunoassay; D008715:Methionine / Q000378:metabolism; D008970:Molecular Weight; D011233:Precipitin Tests; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000378:metabolism*; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis
D012367:RNA, Viral; D014764:Viral Proteins; D008715:Methionine