[Adsorption of enteroviruses on nitrate cellulose membranes: influence of mineral salts, detergents and calf serum (author's transl)].
The filtration through cellulose nitrate membranes of a poliovirus type II suspension in distilled water alone or with added mineral salts (NaCl, MgCl2, AlCl3) results in adsorption of the virus. The maximal adsorption is obtained with NaCl. A detergent in the virus suspension or a pretreatment of the membranes with calf serum prevents this adsorption. Elution of the adsorbed enteroviruses is achieved with alcaline and proteinaceous buffer.
D000327:Adsorption; D000535:Aluminum / Q000494:pharmacology; D000818:Animals; D001769:Blood; D002417:Cattle; D002482:Cellulose; D004770:Enterovirus / Q000302:isolation & purification; D008274:Magnesium / Q000494:pharmacology; D008851:Micropore Filters; D017955:Poliovirus / Q000302:isolation & purification; D011136:Polysorbates / Q000494:pharmacology; D012492:Salts; D012965:Sodium Chloride / Q000494:pharmacology
D011136:Polysorbates; D012492:Salts; D012965:Sodium Chloride; D002482:Cellulose; D000535:Aluminum; D008274:Magnesium
Adsorption d'entérovirus sur des membranes en nitrate de cellulose: influence de sels minéraux, de détergents et du sérum de veau