Quantitation of estradiol receptors employing a sensitive radioimmunoassay.
A method aimed at the identification and quantitation of estradiol receptors has been developed. The test is based on competitive binding reaction between solubilized cell receptors and anti-estradiol antibodies bound to acrylamide beads, for 3H-estradiol. The technique can be employed both for estradiol radioimmunoassay and quantitation of specific receptors in tissues and appears to be a sensitive, specific and reproducible method which can be applied to the evaluation of any hormone and its receptor.
D000818:Animals; D000918:Antibody Specificity; D001667:Binding, Competitive; D003429:Cross Reactions; D003600:Cytosol; D004958:Estradiol / Q000032:analysis* / Q000378:metabolism; D005260:Female; D011817:Rabbits; D011863:Radioimmunoassay; D051381:Rats; D011956:Receptors, Cell Surface; D014316:Tritium; D014599:Uterus / Q000166:cytology
D011956:Receptors, Cell Surface; D014316:Tritium; D004958:Estradiol