Inhibition studies of alkaline phosphatase in hard tissue-forming cells.
The effects of the alkaline phosphatase inhibitors levamisole and R 8231 on p-nitro-phenylphosphatase, inorganic pyrophosphatase and adenosine triphosphatase (ATPase) activities in dentingenically active odontoblasts were studied. The p-nitrophenylphosphatase and inorganic pyrophosphatase activities were inhibited, while 40% of the ATP-splitting enzyme activity remained under the assay condition used. This finding, togeather with earlier studies, indicates that at least two different phosphatase are active at alkaline pH in hard tissue-forming cells; on nonspecific alkaline phosphatase and one specific ATPase. The ATPase activity is uninfluenced by ouabain and ruthenium red and is activated by Ca-2+ ions.
D009597:4-Nitrophenylphosphatase / Q000037:antagonists & inhibitors; D000251:Adenosine Triphosphatases / Q000037:antagonists & inhibitors; D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors*; D000818:Animals; D003810:Dentinogenesis; D006651:Histocytochemistry; D007093:Imidazoles / Q000494:pharmacology; D007180:Incisor / Q000201:enzymology; D007978:Levamisole / Q000494:pharmacology*; D008297:Male; D009804:Odontoblasts / Q000201:enzymology*; D010042:Ouabain / Q000494:pharmacology; D011755:Pyrophosphatases / Q000037:antagonists & inhibitors; D051381:Rats; D012430:Ruthenium Red / Q000494:pharmacology; D013773:Tetramisole / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology*
D007093:Imidazoles; D013844:Thiazoles; D012430:Ruthenium Red; D007978:Levamisole; D010042:Ouabain; D013773:Tetramisole; D000469:Alkaline Phosphatase; D009597:4-Nitrophenylphosphatase; D000251:Adenosine Triphosphatases; D011755:Pyrophosphatases