DNA-binding proteins induced by herpes simplex virus type 2 in HEp-2 cells.
Affinity chromatography on single-stranded and double-stranded DNA-cellulose indicates that 12 proteins previously identified from herpes simplex virus type 2-infected cells, ranging in molecular weight from 28 X 10(3) to 186 X 10(3), bind to DNA-cellulose. The DNA-binding proteins found in infected cells differed in relative binding strengths for denatured DNA-cellulose. The virus specificity of these DNA-binding proteins was further studied by comparison with DNA-binding proteins isolated from mock-infected cells, and by immunoprecipitation of infected-cell DNA-binding proteins with antisera specific for viral antigens. The promise this technique holds for the purification and study of polypeptides involved in virus DNA replication, recombination, or repair is discussed.
D002460:Cell Line; D002846:Chromatography, Affinity; D004247:DNA / Q000378:metabolism; D008970:Molecular Weight; D009691:Nucleic Acid Denaturation; D010455:Peptides / Q000032:analysis / Q000378:metabolism; D011485:Protein Binding; D018139:Simplexvirus / Q000032:analysis* / Q000378:metabolism; D014764:Viral Proteins / Q000032:analysis* / Q000378:metabolism
D010455:Peptides; D014764:Viral Proteins; D004247:DNA