Induction of permanently proliferating human lymphoblastoid lines by N-methyl-N'-nitro-N-nitrosoguanidine.
N-Methyl-N'-nitro-N-nitrosoguanidine treatment of human peripheral blood mononuclear cells increases the frequency of lymphocyte transformation into permanently proliferating lines. The transformation is dependent on the dose of N-methyl-N-7-nitro-N-nitrosoguanidine and does not occur in the presence of autologous human serum. Thirty-eight of the forty-two established lines resulting fron N-methyl-N'-nitro-N-nitrosoguanidine treatment produced detectable Epstein-Barr virus antigens.
D000956:Antigens, Viral; D001769:Blood; D002455:Cell Division; D002460:Cell Line; D004305:Dose-Response Relationship, Drug; D005455:Fluorescent Antibody Technique; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D007244:Infectious Mononucleosis / Q000276:immunology; D007945:Leukemia, Lymphoid / Q000139:chemically induced* / Q000382:microbiology; D008213:Lymphocyte Activation; D009000:Monocytes / Q000187:drug effects*; D009604:Nitrosoguanidines
D000956:Antigens, Viral; D009604:Nitrosoguanidines