title: Interactions between aerolysin, erythrocytes, and erythrocyte membranes.
abstract: Aerolysin, a hemolytic and lethal exotoxin of Aeromonas hydrophila, was analyzed for amino acids. Assuming 8 histidine residues/mol, the purified toxic protein has, by summation, a molecular weight of 49,000, a value in agreement with earlier estimates by other methods. Erythrocytes from different animal species differ greatly in sensitivity to aerolysin's lytic action. There is some correlation between sensitivity and phosphatidyl choline content. Erythrocyte membranes of different species bind the toxin, and the efficiency of binding is a function of sensitivity to lysis. Binding is temperature independent, is not dependent upon membrane sialic acid, and is decreased by prior treatment with phospholipase C and proteases. Preparations of aerolysin convert substantial amounts of membrane phosphorus to water-soluble form; the conversion is concentration and temperature dependent. Most of the conversion is attributable to contaminating phospholipase(s) that is separable from the toxin. Aerolysin purified by electrophoresis in polyacrylamide gel retains some phospholipase activity, and this activity may or may not be a contaminant.
mesh_terms: D000333:Aeromonas / Q000276:immunology*; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002413:Cations, Divalent; D002462:Cell Membrane / Q000276:immunology; D003016:Clostridium perfringens / Q000201:enzymology; D004586:Electrophoresis; D004912:Erythrocytes / Q000276:immunology*; D006168:Guinea Pigs; D006460:Hemolysin Proteins / Q000032:analysis; D006657:Histones / Q000032:analysis; D006736:Horses; D008970:Molecular Weight; D009439:Neuraminidase; D010447:Peptide Hydrolases; D010504:Periodic Acid; D010713:Phosphatidylcholines / Q000032:analysis; D010740:Phospholipases; D010743:Phospholipids / Q000032:analysis; D010758:Phosphorus / Q000032:analysis / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D012756:Sheep; D012964:Sodium; D013696:Temperature
chemical_list: D000596:Amino Acids; D002413:Cations, Divalent; D006460:Hemolysin Proteins; D006657:Histones; D010713:Phosphatidylcholines; D010743:Phospholipids; D010504:Periodic Acid; D010758:Phosphorus; D012964:Sodium; D010740:Phospholipases; D009439:Neuraminidase; D010447:Peptide Hydrolases