Solubility characteristics of Micrococcus lysodeikticus membrane components in detergents and chaotropic salts analyzed by immunoelectrophoresis.
In order to evalute the effectiveness and selectivity of various reagents in the solubilization of bacterial membranes, membranes of Micrococcus lysodeikticus were treated with detergents and chaotropic agents. The composition of the extracts so obtained was analyzed by rocket and two-dimensional immunoelectrophoretic techniques. Recoveery of succinate-, malate-, and reduced nicotinamide adenine dinucleotide- (NADH) dehydrogenases, ATPase, succinylated lipomannan and cytochromes in the extracts was measured. Treatment with a variety of non-denaturing detergents produced extracts that were generally qualitatively uniform although quantitative differences were observed. The degree of extraction of various components was correlated with the hydrophile-lipophile balance. Several chaotropic agents were also evaluated as reagents for membrane solubilization. These agents were less effective in extraction of bulk protein, but produced extracts enriched in some membrane components.
D000251:Adenosine Triphosphatases / Q000032:analysis; D002462:Cell Membrane / Q000032:analysis* / Q000201:enzymology; D003580:Cytochromes / Q000032:analysis; D003902:Detergents; D007122:Immunoelectrophoresis; D008291:Malate Dehydrogenase / Q000032:analysis; D008351:Mannans / Q000032:analysis; D008565:Membrane Proteins / Q000032:analysis; D008837:Micrococcus / Q000032:analysis* / Q000201:enzymology; D009247:NADH, NADPH Oxidoreductases / Q000032:analysis; D012492:Salts; D012995:Solubility; D013385:Succinate Dehydrogenase / Q000032:analysis
D003580:Cytochromes; D003902:Detergents; D008351:Mannans; D008565:Membrane Proteins; D012492:Salts; D008291:Malate Dehydrogenase; D013385:Succinate Dehydrogenase; D009247:NADH, NADPH Oxidoreductases; D000251:Adenosine Triphosphatases