Studies on phosphoglyceromutase from chicken breast muscle: number and reactivity of sulfhydryl groups.
Phosphoglyceromutase (PGM) from chicken breast muscle was titrated with p-mercuribenzoate (PMB), 5,5'-dithiobisnitrobenzoate (Nbs2), N-ethylmaleimide (NEM), iodoacetate and iodoacetamide. The effect of all of the sulfhydryl reagents, with the exception of NEM was to cause a loss in enzymatic activity. Addition of KCN following reaction with Nbs2 resulted in the recovery of a small amount of enzymatic activity. In the absence of substrate (3-phosphoglyceric acid) or cofactor (2,3-diphosphoglyceric acid) and in the presence or absence of 6 M guanidine hydrochloride, six sulfhydryl groups per mole of enzyme were titrated with PMB.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001665:Binding Sites; D002645:Chickens; D002942:Circular Dichroism; D003486:Cyanides; D004228:Dithionitrobenzoic Acid / Q000494:pharmacology; D005033:Ethylmaleimide / Q000494:pharmacology; D007460:Iodoacetamide / Q000494:pharmacology; D007461:Iodoacetates / Q000494:pharmacology; D007700:Kinetics; D008626:Mercuribenzoates / Q000494:pharmacology; D009132:Muscles / Q000201:enzymology*; D010736:Phosphoglycerate Mutase / Q000378:metabolism; D010770:Phosphotransferases / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D013056:Spectrophotometry, Ultraviolet
D000596:Amino Acids; D003486:Cyanides; D007461:Iodoacetates; D008626:Mercuribenzoates; D004228:Dithionitrobenzoic Acid; D010770:Phosphotransferases; D010736:Phosphoglycerate Mutase; D005033:Ethylmaleimide; D007460:Iodoacetamide