[Study of mechanisms of regulation of disulphide reductase in mouse liver].
An increase in activity of disulphide reductase system (DRS) in supernatant of liver tissue was caused by 3',5'-AMP, ATP, GTP, UTP, Mg2+, Ca2+, EDTA, protamine, noradrenaline and F-. The effect was connected with arsenite resistant fraction of DRS. After rapid homogenization the effect of noradrenaline disappeared and the effects of ATP, GTP, UTP and Ca2+ were distinctly decreased. Treatment with adsorbents prevented the effects of 3',5,-AMP, ATP and EDTA and markedly decreased the effects of protamine and Mg2+. A protein inhibitor of protein kinase prevented completely the activation of DRS with 3',5'-AMP, ATP, GTP, UTP and noradrenaline and distinctly decreased the effect of protamine, Mg2+ and Ca2+ but did not alter the influence of EDTA. Addition of 2',3'-AMP blocked the effect of 3',5-AMP, ATP and Mg2+ but did not influence the effect of protamine and EDTA. The data obtained suggest that protein kinase participated in activation of DRS by most of the regulators.
D000227:Adenine Nucleotides / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D000242:Cyclic AMP / Q000494:pharmacology; D004220:Disulfides / Q000378:metabolism; D004492:Edetic Acid / Q000494:pharmacology; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008274:Magnesium / Q000494:pharmacology; D051379:Mice; D010088:Oxidoreductases / Q000378:metabolism*; D014500:Uracil Nucleotides / Q000494:pharmacology
D000227:Adenine Nucleotides; D004220:Disulfides; D014500:Uracil Nucleotides; D004492:Edetic Acid; D000242:Cyclic AMP; D010088:Oxidoreductases; D008274:Magnesium; D002118:Calcium
Issledovanie mekhanizmov reguliatsii disul'fidreduktaznoĭ sistemy pecheni myshi