Type 1 and type 2 herpes simplex uvietis in immune rabbits: importance of pure virus preparation.
Systemic immunization with type 1 herpes simplex virus (HSV) protected rabbits from developing uveitis when they were challenged intravitreally with either purified type 1 or purufied type 2 HSV. But when an HSV preparation contaminated with a nonviral foreign antigen (fetal calf serum) was used for both systemic immunization and challenge, the immunization against HSV failed to exert a protective effect because the fetal calf serum had produced a nonviral immunologic reaction in the uveal tract. This clearly indicates the importance of preparing virus properly for use in experiments of this nature.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D000956:Antigens, Viral; D002417:Cattle / Q000276:immunology; D007114:Immunization; D007271:Injections, Intradermal; D007635:Keratitis, Dendritic; D008297:Male; D011817:Rabbits; D018139:Simplexvirus / Q000145:classification / Q000276:immunology / Q000302:isolation & purification*; D014605:Uveitis / Q000276:immunology / Q000382:microbiology*; D014822:Vitreous Body
D000914:Antibodies, Viral; D000956:Antigens, Viral