DRw antisera react with activated T cells.
Nylon wool-purified T cells appear to be nonreactive in a lymphocytotoxicity assay with HLA-DRw antisera and complement before cell activation. However, after activation in mixed lymphocyte culture, responder cells express determinants that are strongly reactive with DRw alloantisera after 6 days and gradually disappear by 16 to 18 days. Restimulation of the primed cells resulted in re-expression of the blast determinants. Mitogenic stimulation with Con A or purified PHA (HA-17) also resulted in temporary expression of these determinants; reactivity usually conformed to DRw genetic restriction; however, occasional extra reactions occurred that were variable depending on the method of activation (i.e., MLC, Con A, or HA-17). The results suggest the presence of additional allospecificities within some of the DRw antisera that react with "Ia-like" antigens on activated cells from unique subsets of T cells. Whether these DRw antisera contain antibodies against T cells or agains activation or differentiation T cell antigens is not as yet clear.
D000328:Adult; D003208:Concanavalin A / Q000494:pharmacology; D006680:HLA Antigens / Q000276:immunology*; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology*; D007700:Kinetics; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D010835:Phytohemagglutinins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology*
D006680:HLA Antigens; D007106:Immune Sera; D010835:Phytohemagglutinins; D003208:Concanavalin A