Polyoma virus T antigen. I. Synthesis of modified heat-labile T angiten in cells transformed with the ts-a mutant.
In mouse cells transformed with the ts-a mutant of polyoma virus (ts-a-3T3), only low amounts of the virus-specific T antigen were synthesized at high temperature (39 C). After a shift-down to the permissive temperature (31 C), these cells exhibited the same level of T-antigen production as wild-type polyoma transformants. The T antigen produced by ts-a-transformed cells was inactivated at 39 C in vitro at a faster rate than that produced by wild-type-transformed cells. These observations indicate that T antigen is, or includes, a virus-coded peptide.
D000818:Animals; D000951:Antigens, Neoplasm; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002999:Clone Cells; D003168:Complement Fixation Tests; D005347:Fibroblasts; D005455:Fluorescent Antibody Technique; D051379:Mice; D009154:Mutation; D011120:Polyomavirus / Q000254:growth & development / Q000276:immunology*; D013696:Temperature
D000951:Antigens, Neoplasm