[Inhibitory analysis of the respiration of bacteroids from the nodules of yellow lupine].
Oxygen uptake and reduction of C2H2 by bacteroids was found to be inhibited by low concentrations of cyanide and azide. However, oxygen uptake was not completely suppressed even by 10(-3) M KCN. Cyanide-resistant respiration was not inhibited by salicyl-hydroxamic acid, and seemed to be accomplished at the account of autoxidable flavo-proteins. A small light-reversible inhibition of respiration by carbon monoxide was found only in the bacteroids with a high rate of nitrogen fixation. Rotenone, antimycin A, and tenoyltrifluoroacetone inhibited oxygen uptake and methylene reduction. Nitrogen fixation, but not respiration, was inhibited by 2,4-dinitrophenol. An electron-transport chain coupled with phosphorylation is supposed to be built into the membranes of the bacteroids. The activity of peroxidase and cytochrome peroxidase was demonstrated in the bacteroids.
D000968:Antimycin A / Q000494:pharmacology; D001386:Azides / Q000494:pharmacology; D002248:Carbon Monoxide; D003486:Cyanides / Q000494:pharmacology; D004140:Dinitrophenols / Q000494:pharmacology; D004579:Electron Transport; D003576:Electron Transport Complex IV / Q000378:metabolism; D006877:Hydroxamic Acids / Q000494:pharmacology; D008027:Light; D009586:Nitrogen Fixation; D010101:Oxygen Consumption; D010544:Peroxidases / Q000378:metabolism; D012231:Rhizobium / Q000201:enzymology / Q000378:metabolism*; D012402:Rotenone / Q000494:pharmacology; D012459:Salicylates / Q000494:pharmacology; D013804:Thenoyltrifluoroacetone / Q000494:pharmacology
D001386:Azides; D003486:Cyanides; D004140:Dinitrophenols; D006877:Hydroxamic Acids; D012459:Salicylates; D012402:Rotenone; D013804:Thenoyltrifluoroacetone; D000968:Antimycin A; D002248:Carbon Monoxide; D010544:Peroxidases; D003576:Electron Transport Complex IV
Ingibitornyĭ analiz dykhaniia bakteroidov iz kluben'kov zheltogo liupina