[Effect of ultrasound on MG 2+, Na+, K+-ATPase and K+-acetylphosphatase activities of brain subcellular membrane structures].
Mg2+, Na+, K+-ATPase and K+-acetylphosphatase activities in the brain subcellular membrane structures were studied as affected by ultrasound. Effect of ultrasound depends both on the conditions of sonication and the initial state of membrane fractions. The ATPase activity in the NaI-treated microsomal fraction decreases after sonication. The treatment of microsomal myelin, synaptosomal and mitochondrial fractions with EDTA solutions (5-10(-5)--2-10(-2) M) causes an inhibition in the Mg2+, Na+, K+-ATPase. The ATPase activity of these so-called EDTA-fractions increases after a short-term sonication. Activation of the Mg2+, Na+, K+-ATPase of EDTA-microsomal fraction with a sonication is accompanied by an activation of K+-acetylphosphatase. In contrast to the ATPase activity, the acetylphosphatase one is manifested after a longer effect of ultrasound as well. The maximum of acetylphosphatase activation with sonication coincides with the maximum of Mg2+, Na+, K+-ATPase activation. The content of unmasked SH-groups in the studied fractions decreases after sonication. When EDTA-fractions are affected by sonication, which is accompanied by an increase in the ATPase activity, the number of SH-groups in all the fractions, except for mitochondria, increases.
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D001921:Brain / Q000201:enzymology*; D004492:Edetic Acid / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D008274:Magnesium / Q000494:pharmacology; D008566:Membranes / Q000187:drug effects / Q000201:enzymology*; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D011188:Potassium / Q000494:pharmacology; D011485:Protein Binding; D011487:Protein Conformation; D011817:Rabbits; D012964:Sodium / Q000494:pharmacology; D013347:Subcellular Fractions / Q000201:enzymology; D013438:Sulfhydryl Compounds / Q000032:analysis; D014465:Ultrasonics
D013438:Sulfhydryl Compounds; D004492:Edetic Acid; D012964:Sodium; C018735:acetylphosphatase; D010744:Phosphoric Monoester Hydrolases; D000251:Adenosine Triphosphatases; D008274:Magnesium; D011188:Potassium
Vpliv ul'trazvuku na Mg2+, Na+, K+-ATF-aznu ta K+-atsetilfosfataznu aktivonosti subklitinnikh membrannikh struktur golovnogo mozku