title: Mutants of herpes simplex virus types 1 and 2 that are resistant to phosphonoacetic acid induce altered DNA polymerase activities in infected cells.
abstract: Three mutants of herpes simplex virus (HSV) have been isolated which form plaques in the presence of 100 mug/ml phosphonoacetic acid (PPA). All three mutants (3 from HSV-1 strain 17 syn+, 14 from HSV-1 strain 17 syn, and 19 from HSV-2) induce viral DNA synthesis and viral DNA polymerase activity, and these are much less sensitive to PPA than the wild-type virus. The results support the hypothesis that PPA interacts directly with the viral DNA polymerase protein, at least part of which is virus coded.
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D004254:DNA Nucleotidyltransferases / Q000096:biosynthesis*; D004279:DNA, Viral / Q000096:biosynthesis; D004352:Drug Resistance, Microbial; D004790:Enzyme Induction; D005796:Genes; D009154:Mutation; D009943:Organophosphorus Compounds / Q000494:pharmacology*; D018139:Simplexvirus / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D013696:Temperature
chemical_list: D000085:Acetates; D000998:Antiviral Agents; D004279:DNA, Viral; D009943:Organophosphorus Compounds; D004254:DNA Nucleotidyltransferases