title: Cytotoxic and proliferative lymphocyte responses to allogeneic and xenogeneic antigens in vitro.
abstract: In vitro lymphoproliferative responses to foreign histocompatibility antigens are phylogenetically restricted. Responses occur most readily to allogeneic or closely related xenogeneic leucocytes, but not to unrelated xenogeneic cells. Specific cytotoxic T cell responses to foreign histocompatibility antigens show the same phylogenetic restriction. This lack of xenoreactivity is not due to a lack of precursor cells for the xenoantigens; guinea-pig lymphocytes, although normally unresponsive to mouse antigens, have a similar precursor frequency for these antigens as do lymphocytes of allogeneic mouse strains. Specific cytotoxic responses of guinea-pig lymphocytes to mouse antigens can be generated if a factor released from con A stimulated guinea-pig spleen cells is added to the culture medium. The factor produced by con A-activated spleen cells (CS) is also phylogenetically restricted in its action; CS must be obtained from animals homologous with the donor of the responding lymphocytes.
mesh_terms: D000818:Animals; D015478:Antigens, Heterophile / Q000276:immunology*; D003037:Cobalt Radioisotopes; D003601:Cytotoxicity Tests, Immunologic; D006041:Goats; D006168:Guinea Pigs; D006649:Histocompatibility Antigens / Q000276:immunology*; D066298:In Vitro Techniques; D007519:Isoantigens / Q000276:immunology*; D007962:Leukocytes / Q000276:immunology*; D008198:Lymph Nodes / Q000166:cytology; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D051379:Mice; D010802:Phylogeny; D051381:Rats; D012756:Sheep; D013154:Spleen / Q000166:cytology
chemical_list: D015478:Antigens, Heterophile; D003037:Cobalt Radioisotopes; D006649:Histocompatibility Antigens; D007519:Isoantigens