Structure and synthesis of a lipid-containing bacteriophage. Chemical modifications of bacteriophage PM2 and the resulting alterations in acyl-chain motion in the PM2 membrane.
The nucleocapsid proteins of bacteriophage PM2 and the inner lamella of the lipid bilayer, containing most of the phosphatidlethanolamine residues, were selectively cross-linked in the presence of 0.1-0.5% glutaraldehyde, 5 mM dimethylsuberimidate, or 0.05% toluene 2,4-diisocyanate. The biological activity (p.f.u.) of PM2 modified by these reagents decreased 10(6)-fold in all cases. The spike and coat proteins were selectively cross-linked in the presence of 7.5 mM N,N'-p-phenylenedimaleimide. The biological activity of virus modified by this reagen was unaffected. The electron paramagnetic resonance spectra of fatty acid spin labels incorporated into native and chemically modified viral membranes were qualitatively similar but show quantitative differences. Fixation with glutaraldehyde increased the rigidity of the membrane while Triton X-100 induced a more flexible structure. There was no change in the electron paramagnetic resonance spectrum of virus treated with N,N'-p-phenylenedimaleimide, however.
D001435:Bacteriophages / Q000378:metabolism*; D003485:Cyanates; D004120:Dimethyl Suberimidate; D004578:Electron Spin Resonance Spectroscopy; D005976:Glutaral; D008301:Maleimides; D008566:Membranes; D010714:Phosphatidylethanolamines; D011092:Polyethylene Glycols; D011549:Pseudomonas; D013113:Spin Labels; D013229:Stearic Acids; D013696:Temperature; D014050:Toluene; D014764:Viral Proteins
D003485:Cyanates; D008301:Maleimides; D010714:Phosphatidylethanolamines; D013113:Spin Labels; D013229:Stearic Acids; D014764:Viral Proteins; D004120:Dimethyl Suberimidate; D014050:Toluene; D011092:Polyethylene Glycols; C011328:N,N'-4-phenylenedimaleimide; D005976:Glutaral