Mechanism of cell dissociation with trypsin and EDTA.
The mechanism of cell dissociation with trypsin and EDTA was examined. Cell dissociation was possible when trypsin and EDTA were given simultaneously, when trypsin was given after EDTA treatment, but not when trypsin was given before EDTA treatment.
D000818:Animals; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D004492:Edetic Acid / Q000494:pharmacology*; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000473:pathology; D051381:Rats; D014357:Trypsin / Q000494:pharmacology*; D004124:p-Dimethylaminoazobenzene
D004492:Edetic Acid; D004124:p-Dimethylaminoazobenzene; D014357:Trypsin