title: Manganese requirement of phosphoglycerate phosphomutase and its consequences for growth and sporulation of Bacillus subtilis.
abstract: In the absence of manganese, rapidly metabolizable carbohydrates such as glucose or glycerol are not completely metabolized by Bacillus subtilis growing in a nutrient sporulation medium: 3-phosphoglyceric acid (3PGA) accumulates inside the cells, growth stops at a low cell titer, and normal sporulation remains suppressed (no prespore septa). Upon the addition of manganese, 3PGA disappears, growth resumes, and normal sporulation takes place. These effects results from a specific manganese requirement of phosphoglycerate phosphomutase which catalyzes the interconversion of 3PGA and 2-phosphoglyceric acid (2PGA). Other metal ions cannot replace manganese, for which the enzyme has an apparent Km of 0.22 mM.
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology / Q000254:growth & development* / Q000378:metabolism; D002474:Cell-Free System; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D005994:Glycerophosphates / Q000378:metabolism; D007535:Isomerases / Q000378:metabolism*; D008293:Malates / Q000378:metabolism; D008345:Manganese / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D013171:Spores, Bacterial / Q000254:growth & development
chemical_list: D005994:Glycerophosphates; D008293:Malates; D009243:NAD; D008345:Manganese; D007535:Isomerases; D005947:Glucose; D005990:Glycerol