Quantitation of zearalenone by gas-liquid chromatography on capillary glass columns.
A procedure is described for the quantitation of zearalenone from corn by gas-liquid chromatography. An internal standard, zearalanone, is first mixed with the finely ground corn, followed by extraction with ethyl acetate, purification by thin-layer chromatography and formation of trimethyl silyl ether (TMS) and methyl oxime (MOX) derivatives. Positive identification of zearalenone is based upon the retention times of the TMS and MOX-TMS derivatives being identical with those of the standards, comparison of chromatograms from both derivatives and characteristic twin peaks of zearalenone MOX-TMS. The detection limit of 100 parts per 10(9) zearalenone in corn could only be improved by modification of the described procedure.
D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D005898:Glass; D012118:Resorcinols / Q000302:isolation & purification*; D003313:Zea mays / Q000032:analysis; D015025:Zearalenone / Q000302:isolation & purification*
D012118:Resorcinols; D015025:Zearalenone