Cross-reactions of normal bovine serums to foot-and-mouth disease virus in plaque-reduction neutralization and radial immunodiffusion.
Serums from 150 cattle with no known exposure to foot-and-mouth disease (FMD) virus were tested by both the plaque-reduction neutralization (PRN) technique and the radial immunodiffusion (RID) technique to evaluate the significance and the extent of cross-reactions in these tests. Serums from 30 cattle from each of 5 locations were tested against representative viruses of each of the 7 types of FMD virus. High levels of cross-reactions with both the RID and PRN techniques were found in serums of specific groups. Higher levels of cross-reactions were noted for Asia, SAT I, SAT II, and SAT III than for A, O, or C viruses with the PRN technique, and higher levels for SAT II and SAT III with the RID technique. The reactions for most serums appeared to be quite specific; a given serum was often reactive with only 1 or 2 viruses.
D000818:Animals; D005537:Aphthovirus / Q000276:immunology*; D002417:Cattle / Q000276:immunology*; D003429:Cross Reactions; D007106:Immune Sera; D005779:Immunodiffusion; D007484:Iowa; D007564:Japan; D009500:Neutralization Tests; D009518:New York; D009664:Norway; D010948:Viral Plaque Assay
D007106:Immune Sera