Protein synthesis in bacteriophage ghost-infected cells.
Escherichia coli B infected with T4 phage ghosts at 10 mM Mg2+ regains its protein synthesizing activity upon addition of ATP, GTP, and their generator to approximately 2% of the intact exponentially growing cells. In contrast to amino acid incorporation by intact cells, this system is sensitive to EDTA or low Mg2+. On the other hand, this system, differing from the regular cell-free system, does not respond to addition of soluble protein and ribonuclease. The ghost-infected cells were able to synthesize beta-galactosidase upon addition of the inducer isopropyl thiogalactoside. The initial rate of the induction was 2.6% of intact cells. For this induction, the addition of cyclic AMP, amino acids, ATP, GTP, UTP, CTP, and their generator was necessary. The induction of beta-galactosidase in these ghost-infected cells was very sensitive to the addition of EDTA, CaCl2, sulfhydryl blocking reagent, rifampin and chloramphenicol but insensitive to DNA synthesis inhibitors such as nalidixic acid and DNase.
D000596:Amino Acids / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis*; D002122:Calcium Chloride / Q000494:pharmacology; D002701:Chloramphenicol / Q000494:pharmacology; D003090:Coliphages; D000242:Cyclic AMP / Q000378:metabolism; D004492:Edetic Acid / Q000494:pharmacology; D004790:Enzyme Induction; D004926:Escherichia coli / Q000201:enzymology / Q000378:metabolism*; D005696:Galactosidases / Q000096:biosynthesis; D007544:Isopropyl Thiogalactoside / Q000378:metabolism; D008274:Magnesium / Q000494:pharmacology; D009711:Nucleotides / Q000378:metabolism; D012260:Ribonucleases / Q000494:pharmacology; D012293:Rifampin / Q000494:pharmacology; D013439:Sulfhydryl Reagents / Q000494:pharmacology; D014764:Viral Proteins / Q000494:pharmacology*
D000596:Amino Acids; D001426:Bacterial Proteins; D009711:Nucleotides; D013439:Sulfhydryl Reagents; D014764:Viral Proteins; D007544:Isopropyl Thiogalactoside; D002701:Chloramphenicol; D004492:Edetic Acid; D000242:Cyclic AMP; D012260:Ribonucleases; D005696:Galactosidases; D008274:Magnesium; D002122:Calcium Chloride; D012293:Rifampin