Studies of liver phosphorylase in hepatic injuries II. Alteration in isozyme pattern.
Enzyme deviations in injured livers were studied by analyzing isozyme patterns of phosphorylase using a newly developed electrophoretic method, which separates six molecular species of this enzyme, i.e. M,FM,F,L,L', and FL'. In hepatic injuries caused by CCl4 and galactosamine intoxications of rats, F appeared in early stages and L' (and FL') in later stages of the injuries with a concurrent decrease or loss of L, which is a sole isozyme component of adult liver. In injured livers of patients with hepatitis and cirrhosis of the liver, increases in FL' activity were also found. Appearance of F was found only in hepatocellular carcinoma. The results obtained with phosphorylase isozyme analysis support the idea that an undifferentiated gene expression takes place in the injured livers of non-malignant hepatic disorders.
D000818:Animals; D002252:Carbon Tetrachloride Poisoning / Q000201:enzymology; D056486:Chemical and Drug Induced Liver Injury / Q000201:enzymology; D004586:Electrophoresis; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000032:analysis*; D008099:Liver / Q000201:enzymology*; D008107:Liver Diseases / Q000201:enzymology*; D009206:Myocardium / Q000201:enzymology; D006005:Phosphorylases / Q000032:analysis*; D011247:Pregnancy; D051381:Rats
D007527:Isoenzymes; D006005:Phosphorylases