title: Variation in the effects of antidiuretic hormone on the isolated skin of the toad, Scaphiopus couchi.
abstract: The rate of active sodium transport as measured by short-circuit current across the isolated skin of the toad, Scaphiopus couchi, was elevated following vasopressin (0.2 units/ml) or arginine vasotocin (0.1 units/ml) treatment of skins from active animals at all times of the year tested. Skins from dormant animals showed no such elevation at any time of the year. The rate of active sodium transport was elevated following treatment with dibutyryl cyclic AMP (2.5mM) plus theophylline (10 mM) in all skins tested. The hydraulic conductivity of isolated skins from both active and dormant animals showed no significant change following treatment with vasopressin (0.2 units/ml) or arginine vasotocin (0.1 units/ml except on the first day following emergence from dormancy in the field. A correlation was, therefore, observed between the occurrence of a hydroosmotic response to antidiuretic hormones and the seasonal exposure of S. couchi to standing water. A small but significant elevation of hydraulic conductivity was observed across the skins of dormant toads following treatment with dibutyryl cyclic AMP (2.5 mM) plus theophylline (10 mM) whereas a substantial elevation was observed with the skins of active animals.
mesh_terms: D000818:Animals; D001001:Anura / Q000378:metabolism* / Q000502:physiology; D003994:Bucladesine / Q000494:pharmacology; D009995:Osmosis; D012621:Seasons; D012867:Skin / Q000187:drug effects*; D012879:Skin Physiological Phenomena; D012964:Sodium / Q000378:metabolism; D013806:Theophylline / Q000494:pharmacology; D014667:Vasopressins / Q000494:pharmacology*; D014668:Vasotocin / Q000494:pharmacology
chemical_list: D014667:Vasopressins; D003994:Bucladesine; D012964:Sodium; D013806:Theophylline; D014668:Vasotocin