Synthesis of inducible enzyme in Escherichia coli recovering from prolonged energy starvation.
A marked breakdown of ribosomes and rRNA occurs in Escherichia coli cells during prolonged deprivation of a carbon source (energy starvation). In E. coli recovering from energy starvation: (a) synthesis of RNA started immediately, total protein synthesis showed a delay of 5 to 10 minutes; (b) beta-galactosidase, tryptophanase and serine deaminase could not be induced in the first 50--70 min; (c) a lag of 60 min in the synthesis of beta-galactosidase was observed in a lac constitutive mutant of E. coli; synthesis of the constitutive enzyme malate dehydrogenase did not shown any delay. RNA synthesized in the early stages of recovery contained a higher percentage of low molecular weight molecules than RNA synthesized after 70 min of recovery or during exponential growth. Messenger RNA specific for beta-galactosidase was not synthesized for the first 50--60 min of recovery even when the specific inducer was added to the cultures.
D002244:Carbon / Q000378:metabolism*; D002455:Cell Division; D002470:Cell Survival; D003090:Coliphages / Q000378:metabolism; D000242:Cyclic AMP / Q000494:pharmacology; D004269:DNA, Bacterial / Q000378:metabolism; D004790:Enzyme Induction / Q000187:drug effects; D004926:Escherichia coli / Q000378:metabolism*; D005696:Galactosidases / Q000096:biosynthesis*; D012695:L-Serine Dehydratase / Q000096:biosynthesis*; D008190:Lyases / Q000096:biosynthesis*; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D012329:RNA, Bacterial / Q000096:biosynthesis; D013997:Time Factors; D014158:Transcription, Genetic; D014368:Tryptophanase / Q000096:biosynthesis*
D004269:DNA, Bacterial; D012329:RNA, Bacterial; D002244:Carbon; D000242:Cyclic AMP; D005696:Galactosidases; D008190:Lyases; D014368:Tryptophanase; D012695:L-Serine Dehydratase