Structure of heparan sulphate oligosaccharides and their degradation by exo-enzymes.
Oligosaccharides obtained from heparan sulphate by nitrous acid degradation were shown to be degraded sequentially by beta-D-glucuronidase or alpha-L-iduronidase followed by alpha D-N-acetylglucosaminidase. Structural analysis of the tetrasaccharide fraction showed the following. (1) N-Acetylglucosamine is preceded by a non-sulphated uronic acid residue that can be either D-glucuronic of L-iduronic acid, but followed by a glucuronic acid residue. (2) The N-acetylglucosamine in the major fraction is sulphated. (3) Very few if any of the uronic acid residues are sulphated (4). The results indicate that the area of the heparan sulphate chain where disaccharides containing N-acetylglucosamine and N-sulphated glucosamine residues alternate is higher in sulphate content than expected and that the sulphate groups are mainly located on the hexosamine units.
D000118:Acetylglucosaminidase; D055598:Chemical Phenomena; D002621:Chemistry; D002854:Chromatography, Paper; D005417:Flavobacterium / Q000201:enzymology; D005966:Glucuronidase; D006025:Glycosaminoglycans; D006026:Glycoside Hydrolases; D006497:Heparan Sulfate; D006868:Hydrolysis; D007068:Iduronidase; D008956:Models, Chemical; D009608:Nitrous Acid; D009844:Oligosaccharides / Q000032:analysis*
D000118:Acetylglucosaminidase; D005966:Glucuronidase; D006025:Glycosaminoglycans; D006026:Glycoside Hydrolases; D006497:Heparan Sulfate; D007068:Iduronidase; D009608:Nitrous Acid; D009844:Oligosaccharides