A method for the quantitative determination of glycerolipids containing O-alkyl and O-alk-1-enyl moieties.
We have developed a spectrophotometric procedure, based on a combination of established methods, for the quantitative determination of aklyl and alk-1-enyl (plasmalogens) ether-linked glycerolipids. It depends upon the release of alkylglycerols and alk-1-enylglycerols from phospholipids by phosphlipase C (Bacillus cereus) followed by saponification or by Vitride reduction the phospholipids; aldehydes are subsequently formed and measured colorimetrically after reacting them with a fuchsin reagent. The total alkyl and alk-1-enyl content of glycerolipids is determined oxidation of the sample withperiodate to form aldehydes and alkylglycolic aldehydes. The O-alk-1-enyl lipid content is determined on a separate sample by measuring the aldehydes produced after acid hydrolysis. The quantity of O-alkyl lipids is calculated from the difference between the values obtained for the total ether-lipid content and that of the O-alk-1enyl lipid content. Alternately, direct determination of alk-1-enylglycerols and alkylglycerols can be made if these hydrolytic products are first separated by thin-layer chromatography.
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000032:analysis; D005231:Fatty Acids, Unsaturated / Q000032:analysis; D005988:Glyceric Acids / Q000032:analysis*; D005989:Glycerides / Q000032:analysis; D008055:Lipids / Q000032:analysis*; D008099:Liver / Q000032:analysis; D008113:Liver Neoplasms; D008297:Male; D008722:Methods; D009374:Neoplasms, Experimental / Q000032:analysis; D009928:Organ Specificity; D010740:Phospholipases; D010743:Phospholipids / Q000032:analysis; D010955:Plasmalogens / Q000032:analysis; D051381:Rats
D005231:Fatty Acids, Unsaturated; D005988:Glyceric Acids; D005989:Glycerides; D008055:Lipids; D010743:Phospholipids; D010955:Plasmalogens; D010740:Phospholipases