Adaptation of N-tropic Friend leukaemia virus and its murine sarcoma virus pseudotype of non-permissive B-type C57BL/6 mouse cell line.
N-tropic Friend leukaemia virus (FLV) was serially passaged in a B-type C57BL/6 mouse cell line, YH-7. After a single passage, the infectious efficiency of FLV in the restrictive YH-7 cells was significantly increased. This adapted character of FLV could be reversed by a single passage in permissive N-type MLg cells. The true host range conversion from N to NB was accomplished after eleven to twelve passages in YH-7 cells. In contrast, the host range conversion of N-tropic murine sarcoma pseudotype, MuSV(FLV), took place after two passages.
D000220:Adaptation, Biological; D000818:Animals; D002460:Cell Line; D005622:Friend murine leukemia virus / Q000254:growth & development*; D017988:Gammaretrovirus / Q000254:growth & development*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009053:Sarcoma Viruses, Murine / Q000254:growth & development*; D013045:Species Specificity; D014779:Virus Replication