title: Enzymic comparisons of the inorganic sulfur metabolism in autotrophic and heterotrophic Thiobacillus ferrooxidans.
abstract: Activities of enzymes which mediate the oxidation of thiosulfate to sulfate and the assimilation of sulfate to sulfide were assayed in various cell-free fractions of Thiobacillus ferrooxidans grown autotrophically on either ferrous iron or thiosulfate or heterotrophically on glucose. There was no activity of the thiosulfate-oxidizing enzyme in extracts of bacteria grown with ferrous iron. Comparable activities for ATP-sulfurylase (EC 2.7.7.4), ADP-sulfurylase (EC 2.7.7.5), and adenylate kinase (EC 2.7.4.3) were found in the bacteria grown autotrophically with either Fe2+ or S2O32- or heterotrophically with glucose.
mesh_terms: D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000263:Adenylate Kinase / Q000378:metabolism*; D002474:Cell-Free System; D007501:Iron / Q000378:metabolism; D009713:Nucleotidyltransferases / Q000378:metabolism*; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism*; D010770:Phosphotransferases / Q000378:metabolism*; D013431:Sulfates / Q000378:metabolism; D013455:Sulfur / Q000378:metabolism*; D013466:Sulfurtransferases / Q000378:metabolism*; D013855:Thiobacillus / Q000201:enzymology* / Q000378:metabolism; D013884:Thiosulfate Sulfurtransferase / Q000378:metabolism*; D013885:Thiosulfates / Q000378:metabolism
chemical_list: D013431:Sulfates; D013885:Thiosulfates; D013455:Sulfur; D000255:Adenosine Triphosphate; D007501:Iron; D010088:Oxidoreductases; D010770:Phosphotransferases; D000263:Adenylate Kinase; D009713:Nucleotidyltransferases; D013466:Sulfurtransferases; D013884:Thiosulfate Sulfurtransferase