The 5' end group of tobacco mosaic virus RNA is m7G5' ppp5' Gp.
RNA extracted from CsC1-purified virions of tobacco mosaic virus is shown to give rise to an unusual nucleotide on digestion which RNAase T2, in addition to the four major nucleotides. This minor component has the electrophoretic characteristics of a phosphorylated end group, but is partially resistant to bacterial alkaline phosphatase. It is, however, a substrate for venom phosphodiesterase or nucleotide pyrophosphatase, yielding products which imply the structure m7G5'ppp5'Gp. TMV RNA, like many animal cellular and viral mRNAs recently examined, therefore has a 5' terminus blocked by a methylated nucleotide inverted with respect to the rest of the chain.
D000469:Alkaline Phosphatase; D000818:Animals; D001483:Base Sequence; D002855:Chromatography, Thin Layer; D003090:Coliphages / Q000201:enzymology; D004926:Escherichia coli / Q000201:enzymology; D006150:Guanine Nucleotides / Q000032:analysis*; D009711:Nucleotides; D010179:Pancreas / Q000201:enzymology; D010727:Phosphoric Diester Hydrolases; D011755:Pyrophosphatases; D012367:RNA, Viral / Q000032:analysis* / Q000302:isolation & purification; D012260:Ribonucleases; D012911:Snakes; D014027:Tobacco Mosaic Virus / Q000032:analysis*; D014688:Venoms
D006150:Guanine Nucleotides; D009711:Nucleotides; D012367:RNA, Viral; D014688:Venoms; D012260:Ribonucleases; D000469:Alkaline Phosphatase; D010727:Phosphoric Diester Hydrolases; D011755:Pyrophosphatases