Suppressor-induced structural changes in a missense L-ribulokinase of Escherichia coli.
A suppressor mutation specific for a missense codon in the L-ribulokinase structural gene of the L-arabinose operon of Escherichia coli B/r enhanced L-arabinose utilization by the strain containing the missense codon. Electrophoretic comparisons of the wild-type, missense, and suppressed missense L-ribulokinases indicated that the suppressor changed the structure of the missense kinase, thereby increasing its catalytic activity. Hyperinducibility imposed on an operator-distal gene by the missense codon was not affected by the suppressor mutation.
D001089:Arabinose / Q000378:metabolism; D002238:Carbohydrate Epimerases / Q000378:metabolism; D002474:Cell-Free System; D004926:Escherichia coli / Q000201:enzymology* / Q000378:metabolism; D005796:Genes; D009154:Mutation; D010770:Phosphotransferases / Q000096:biosynthesis* / Q000378:metabolism; D013237:Stereoisomerism; D013489:Suppression, Genetic; D014161:Transduction, Genetic
D001089:Arabinose; D010770:Phosphotransferases; D002238:Carbohydrate Epimerases