Cell rigidity: Effect on concanavalin A-mediated agglutinability of fibroblasts after fixation.
A quantitative hemadsorption assay distinguishes the effects of membrane fixation on concanavalin A-mediated agglutinability of fixed cells with unfixed cells. We observed undiminished adherence of unfixed erythrocytes to glutaraldehyde-fixed normal and virus-transformed hamster fibroblasts coated with concanavalin A. Fixation of the erythrocytes abolished agglutination with fixed fibroblasts. The agglutinability of fixed cells is more likely related to increased cell rigidity than to decreased membrane fluidity.
D000371:Agglutination; D000818:Animals; D002448:Cell Adhesion; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology*; D006224:Cricetinae; D003584:Cytological Techniques; D004622:Embryo, Mammalian; D004912:Erythrocytes; D005347:Fibroblasts; D005976:Glutaral; D006383:Hemadsorption Inhibition Tests; D006386:Hemagglutination Tests; D011120:Polyomavirus
D003208:Concanavalin A; D005976:Glutaral