Granulocytic chalone inhibits rapidly proliferating committed murine progenitor cells (CFU-C).
Bovine leucocyte extract containing the granulocytic chalone inhibited proliferation of rapidly, but not slowly, prliferating murine CFU-C. The precursors of CFU-C, i.e. multipotent colon-forming stem cells (CFU-S), were apparently not affected. Inhibition of proliferation was detected as a decreased killing of DNA synthesizing cells in diffusion chamber cultures by hydroxyurea, after pre-treatment with the extract.
D000818:Animals; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D003114:Colony-Forming Units Assay; D004247:DNA / Q000096:biosynthesis; D003864:Depression, Chemical; D005260:Female; D006098:Granulocytes / Q000032:analysis / Q000166:cytology*; D006131:Growth Inhibitors / Q000097:blood / Q000494:pharmacology*; D006918:Hydroxyurea / Q000494:pharmacology; D008297:Male; D051379:Mice; D051381:Rats
D006131:Growth Inhibitors; D004247:DNA; D006918:Hydroxyurea