Incorporation of isotopic carbon into cerebral glycogen from non-glucose substrates.
1. Measurable incorporation of radioactive carbon from [U-14C]pyruvate, [U-14C]-glutamate and [14C]bicarbonate into the glycogen synthesized by brain slices in vitro was demonstrated. 2. The fructose diphosphatase activity of guinea-pig brain was determined and found to be about 0.03 mumol of substrate degraded/min per g of fresh tissue. 3. The specific radioactivity of the glucose carbon from glycogen relative to that of the precursor added to the incubation medium gave approximate values of 0.195 for glucose, 0.006 for pyruvate, 0.039 for glutamate and 0.001 for bicarbonate.
D000818:Animals; D001639:Bicarbonates / Q000378:metabolism; D001921:Brain / Q000201:enzymology / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism*; D002250:Carbon Radioisotopes; D002854:Chromatography, Paper; D005260:Female; D006597:Fructose-Bisphosphatase / Q000032:analysis; D005947:Glucose / Q000378:metabolism; D005971:Glutamates / Q000378:metabolism; D006003:Glycogen / Q000096:biosynthesis*; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008297:Male; D011773:Pyruvates / Q000378:metabolism
D001639:Bicarbonates; D002250:Carbon Radioisotopes; D005971:Glutamates; D011773:Pyruvates; D002244:Carbon; D006003:Glycogen; D006597:Fructose-Bisphosphatase; D005947:Glucose