title: Investigations on isolated islets of Langerhans in vitro. XIII. Experiments concerning the preparation conditions with collagenase.
abstract: During the digestion of pancreatic pieces with collagenase for prepartion of isolated islets the enzymes in incubation medium (collangenolytic and/or proteolytic) can alter the secretion behavior of A- and B-cells. Insulin release after such an enzymatic attack is characterized by an enhanced basal secretion and a diminished and delayed glucose response. Overdigestion results in a decreased glucagon secretion in response to arginine, a diminished insulin content, and a decreased thiol-protein-disulfide-oxidoreductase activity of the islets. Increased albumin concentrations did not prevent the collagenase effect.
mesh_terms: D000818:Animals; D005260:Female; D005934:Glucagon / Q000378:metabolism; D006652:Histological Techniques; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D003012:Microbial Collagenase / Q000494:pharmacology* / Q000592:standards; D051381:Rats; D012710:Serum Albumin, Bovine
chemical_list: D007328:Insulin; D012710:Serum Albumin, Bovine; D005934:Glucagon; D003012:Microbial Collagenase