Role of Ca2+ and Mg2+ in some human neutrophil functions as indicated by ionophore A23187.
Studies with the divalent cation ionophore A23187 suggest that both Ca2+ and Mg2+ ion influx play a role in human peripheral blood neutrophil function. Degranulation of neutrophils occurred at ionophore concentrations of 10(-5) M and was Ca2+ but not Mg2+ dependent. Modulation of neutrophil chemotaxis was enhanced optimally by 10(-7) M ionophore and was both Ca2+ and Mg2+ dependent. Concentrations of ionophore as low as 10(-12) M seemed to sensitize the cells to a concentration of phorbol myristate acetate which by itself was not chemotactic. These findings also indicate that factors other than Ca2+ or increases in cyclic nucleotides are important to initiation and modulation of neutrophil function.
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000001:Calcimycin / Q000494:pharmacology*; D002118:Calcium / Q000502:physiology*; D002413:Cations, Divalent; D002633:Chemotaxis / Q000187:drug effects; D006152:Cyclic GMP / Q000378:metabolism; D003594:Cytoplasmic Granules / Q000187:drug effects; D006801:Humans; D008274:Magnesium / Q000502:physiology*; D009504:Neutrophils / Q000502:physiology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
D000900:Anti-Bacterial Agents; D002413:Cations, Divalent; D000001:Calcimycin; D006152:Cyclic GMP; D008274:Magnesium; D013755:Tetradecanoylphorbol Acetate; D002118:Calcium