Chromatographic studies on picornavirus capsid polypeptides.
The polypeptides of encephalomyocarditis, Mouse-Elberfeld and type 5 rhinoviruses behave similarly when chromatographed on calcium phosphate (brushite), each being eluted by a linear phosphate buffer gradient containing sodium dodecyl sulphate in three major peaks, CI, C2 and C3. Analysis of the peaks by polyacrylamide gel electrophoresis suggests that the major capsid polypeptides of these three picornaviruses elute in the order: delta (peak CI), gamma with (peak C2) and alpha (peak C3).
D000818:Animals; D002130:Calcium Phosphates; D002250:Carbon Radioisotopes; D002287:Carcinoma, Krebs 2; D002460:Cell Line; D002845:Chromatography; D004591:Electrophoresis, Polyacrylamide Gel; D004686:Encephalomyocarditis virus / Q000032:analysis*; D004770:Enterovirus / Q000032:analysis*; D014644:Genetic Variation; D006367:HeLa Cells; D006801:Humans; D004680:Maus Elberfeld virus / Q000032:analysis*; D051379:Mice; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis*; D012229:Rhinovirus / Q000032:analysis*; D012967:Sodium Dodecyl Sulfate; D014764:Viral Proteins / Q000032:analysis*
D002130:Calcium Phosphates; D002250:Carbon Radioisotopes; D010455:Peptides; D014764:Viral Proteins; D012967:Sodium Dodecyl Sulfate