An improved two dimensional thin-layer chromatography system for the separation of phosphatidylglycerol and its derivatives.
A two dimensional thin-layer chromatography system has been devised for the improved separations of phosphatidylglycerol and its derivaties, cardiolipin and bis/monoacylglyceryl)phosphate, from the other phospholipid components of tissue total lipid extracts. The system employs silica gel G plates prepared with 0.4 M boric acid. Linear recovery of added phosphatidylglycerol was found, and phosphatidylglycerol did not cochromatograph with N, N-dimethylphosphatidylethanolamine in this system. The phospholipid class composition of various rat tissues and a Morris 7777 hepatoma has been determined and compared with values from the literature.
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000032:analysis; D002855:Chromatography, Thin Layer / Q000379:methods; D008113:Liver Neoplasms; D008297:Male; D008832:Microchemistry; D009928:Organ Specificity; D010715:Phosphatidylglycerols / Q000302:isolation & purification*; D010743:Phospholipids / Q000302:isolation & purification*; D051381:Rats; D013329:Structure-Activity Relationship
D010715:Phosphatidylglycerols; D010743:Phospholipids