title: The tissue culture and morphology of human breast tumor cell line BOT-2.
abstract: A continuous human breast tumor cell line (BOT-2) was derived from an infiltrating duct carcinoma. The tumor cell line was grown as a monolayer in flasks, but the cells could be readily adapted to growth in roller cultures. These studies indicate that BOT-2 cells have a 16- to 18-hr doubling time and a modal chromosomal number of 63. The original BOT-2 cell culture has been in continuous cultivation for almost 2 years and has been passed 137 times. The BOT-2 cell line has been differentiated from HeLa cells by isoenzyme studies and chromosomal analysis.
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis; D001943:Breast Neoplasms / Q000201:enzymology / Q000473:pathology*; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000201:enzymology / Q000473:pathology*; D002460:Cell Line; D002875:Chromosomes; D046508:Culture Techniques; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis; D006801:Humans; D007527:Isoenzymes / Q000032:analysis; D007621:Karyotyping; D013997:Time Factors
chemical_list: D007527:Isoenzymes; D005954:Glucosephosphate Dehydrogenase; D000469:Alkaline Phosphatase