A radioisotopic assay for proline oxidase activity.
We developed a radioisotopic assay for proline oxidase in which product deltal-pyrroline-5-carboxylate-14-C is reacted with o-aminobenzaldehyde and the radioactivity trapped as the dihydroquinazolinium compound is recovered by ion-exchange chromatography. The sensitivity of this method allows the measurement of proline oxidase activity in small specimens (10 to 20 mg.) of liver.
D000588:Amines / Q000378:metabolism; D000818:Animals; D001547:Benzaldehydes / Q000378:metabolism; D002250:Carbon Radioisotopes; D002852:Chromatography, Ion Exchange; D003124:Colorimetry; D003574:Cytochrome c Group; D006736:Horses; D008099:Liver / Q000201:enzymology; D008297:Male; D008722:Methods; D010088:Oxidoreductases / Q000032:analysis*; D011392:Proline / Q000302:isolation & purification; D011799:Quinazolines / Q000032:analysis; D051381:Rats
D000588:Amines; D001547:Benzaldehydes; D002250:Carbon Radioisotopes; D003574:Cytochrome c Group; D011799:Quinazolines; D011392:Proline; D010088:Oxidoreductases