[Enzyme kinetic determination of glucose (author's transl)].
A reaction kinetic method is described for the determination of glucose in biological fluids. Glucose is oxidized to gluconic acid and NAD is reduced to NADH in the presence of glucose dehydrogenase. The rate of formation of NADH is followed in a LKB Reaction-Rate-Meter. Together with the analogue read-out, the rate of reaction is determined with a newly developed kinetic calculator. The specifications of the calculator are given. The new method has the advantage of a high rate of throughput and good reproducibility.
D000429:Alcohol Oxidoreductases / Q000378:metabolism; D001322:Autoanalysis; D001786:Blood Glucose / Q000032:analysis; D002374:Catalase / Q000378:metabolism; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D006029:Glycosuria; D006593:Hexokinase / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D009243:NAD; D010544:Peroxidases / Q000378:metabolism; D013053:Spectrophotometry / Q000379:methods; D013997:Time Factors
D001786:Blood Glucose; D009243:NAD; D000429:Alcohol Oxidoreductases; D005954:Glucosephosphate Dehydrogenase; D005949:Glucose Oxidase; D010544:Peroxidases; D002374:Catalase; D006593:Hexokinase; D005947:Glucose
Ein Verfahren zur enzymkinetischen Bestimmung von Glucose