Preparation of anti-rat albumin antiserum and its application to radioimmunoassay.
Albumin was highly purified from a commercially available rat albumin preparation (Fraction V) using disc electrophoresis. The purified albumin had the same antigenicity as Fraction V. A monospecific anti-rat albumin rabbit serum was obtained. The antiserum was used in a double antibody quantitative method for determining rat albumin.
D000418:Albumins / Q000276:immunology* / Q000302:isolation & purification; D000818:Animals; D007106:Immune Sera; D011863:Radioimmunoassay; D051381:Rats / Q000276:immunology
D000418:Albumins; D007106:Immune Sera