Alterations in SVT2 cell transfer RNAs in response to cell density and serum type.
The chromatographic elution profiles of tRNA-A sn, tRNA-A sp, tRNA-H is and tRNA-T y r from SV40-transformed BALB-3T3 cells grown in fetal calf serum or cald serum-supplemented media have been examined. The relative proportions of certain of the isoaccepting species of these four tRNAs are altered in a similar fashion depending on the serum type. It is suggested that the elution profile alterations reflect the extent of modifications of a specific G residue to the minor nucleoside Q, and that this process differs between untransformed and transformed cells. In addition, cell density appears to influence the Q content of these tRNAs, though other density-dependent tRNA modifications also appear to occur.
D000818:Animals; D001216:Asparagine; D001224:Aspartic Acid; D002417:Cattle; D002460:Cell Line / Q000378:metabolism*; D002471:Cell Transformation, Neoplastic; D003470:Culture Media; D003488:Cyanogen Bromide; D006639:Histidine; D051379:Mice; D008807:Mice, Inbred BALB C; D012343:RNA, Transfer / Q000378:metabolism*; D012367:RNA, Viral / Q000378:metabolism*; D013539:Simian virus 40 / Q000378:metabolism*; D014443:Tyrosine
D003470:Culture Media; D012367:RNA, Viral; D001224:Aspartic Acid; D014443:Tyrosine; D006639:Histidine; D001216:Asparagine; D012343:RNA, Transfer; D003488:Cyanogen Bromide