Temperature-sensitive mutants of simian virus 40 selected by transforming ability.
Eight temperature-sensitive mutants of simian virus 40 which transform rat cells at 32.5 C but not at 38.5 C have been isolated. All the mutants were also temperature sensitive for replication in African green monkey kidney cells and five of them were classified into a single complementation group. No mutant incapable of transforming rat cells at either temperature was isolated.
D000818:Animals; D001921:Brain; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002999:Clone Cells; D046508:Culture Techniques; D005816:Genetic Complementation Test; D000882:Haplorhini; D006898:Hydroxylamines; D007668:Kidney; D009153:Mutagens; D009154:Mutation; D051381:Rats; D013539:Simian virus 40 / Q000254:growth & development*; D013696:Temperature; D014466:Ultraviolet Rays; D010948:Viral Plaque Assay; D014779:Virus Replication
D006898:Hydroxylamines; D009153:Mutagens