title: Comparison of two types of endothelial cells in long term culture.
abstract: Bovine aortic endothelial cells have been serially subcultured and maintained for ten months (24 passages) without any apparent loss of endothelial-like properties. There appear to be two endothelial cell types; one type is mononucleated, 40-60 mu in diameter, and the other (giant) cell type, multinucleate and 300-600 mu in diameter. Both cell types were identified as endothelial cells by established criteria; 1. ultrastructurally, by the presence of Weibel-Palade bodies, microfilaments and pinocytotic vesicles and 2. immunologically, by the presence of factor VIII antigen and thrombosthenin.
mesh_terms: D000818:Animals; D000941:Antigens / Q000032:analysis; D001011:Aorta / Q000166:cytology; D001158:Arteries / Q000032:analysis / Q000166:cytology* / Q000648:ultrastructure; D001798:Blood Proteins / Q000032:analysis; D002417:Cattle; D002478:Cells, Cultured; D004727:Endothelium / Q000166:cytology; D005169:Factor VIII / Q000032:analysis; D066298:In Vitro Techniques; D002479:Inclusion Bodies / Q000648:ultrastructure; D009940:Organoids / Q000648:ultrastructure
chemical_list: D000941:Antigens; D001798:Blood Proteins; D005169:Factor VIII