Purification of tryptophan transfer RNA from chick cells and its identity with "spot 1" RNA of Rous sarcoma virus.
Tryptophan transfer RNA from chick cells chromatographs differently in the reversed-phase column chromatographic system 5 depending upon whether it is aminoacylated or not. This property was utilized to prepare pure tryptophan tRNA. Oligonucleotide fingerprints of tryptophan tRNA purified in this manner are identical with those reported for "spot 1" RNA isolated from Rous sarcoma virus.
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000032:analysis* / Q000201:enzymology; D002478:Cells, Cultured; D002642:Chick Embryo; D002852:Chromatography, Ion Exchange; D004588:Electrophoresis, Cellulose Acetate; D009841:Oligonucleotides / Q000032:analysis; D012343:RNA, Transfer / Q000302:isolation & purification*; D012367:RNA, Viral / Q000302:isolation & purification*; D014364:Tryptophan / Q000378:metabolism; D014369:Tryptophan-tRNA Ligase / Q000378:metabolism
D009841:Oligonucleotides; D012367:RNA, Viral; D014364:Tryptophan; D012343:RNA, Transfer; D014369:Tryptophan-tRNA Ligase