Isolation and characterization of a dog serum lipoprotein having apolipoprotein A-I as its predominant protein constituent.
The serum high density lipoproteins (HDL) of normolipemic dogs (beagles) were isolated in the density range of p 1.063 to 1.21 g/ml, and characterized in terms of composition and physical properties (flotation and diffusion coefficients, partial specific volume, molecular weight, electrophoretic mobility, ultraviolet absorption, and circular dichroism). The results indicated that canine HDL is a relatively homogeneous class with a molecular weight of about 230 000 and general properties similar to those reported for human HDL. After delipidation, the resulting apolipoprotein, apo-HDL, was fractionated by Sephadex G-200 column chromatography in urea or guanidine hydrochloride solutions. About 90% of the apo-HDL consisted of a protein with a molecular weight of about 28 000, similar in amino acid composition to human apolipoprotein A-I and having the same NH2 terminus (aspartic acid) and COOH terminus (glutamine) and no carbohydrates. Two other proteins were isolated, one having an apparent mol wt of 55 000 and representing, at least in part, an aggregate of apolipoprotein A-I and the other component with a mol wt of 8000, not yet characterized. The results indicate that canine HDL, as an intact complex, has general physical properties that lie between those reported for human HDL2 and HDL3, and that it differs compositionally from the human products mainly in its predominant content of apo-A-I. These findings together with evidence for the relatively homogeneous nature of the canine HDL provide new prospects for unraveling the relationship between polypeptide composition and HDL structure.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001059:Apoproteins / Q000097:blood* / Q000302:isolation & purification; D002784:Cholesterol / Q000097:blood; D002788:Cholesterol Esters / Q000097:blood; D002942:Circular Dichroism; D004285:Dogs; D004586:Electrophoresis; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005989:Glycerides / Q000097:blood; D006801:Humans; D005779:Immunodiffusion; D008074:Lipoproteins / Q000097:blood* / Q000302:isolation & purification; D008075:Lipoproteins, HDL / Q000097:blood; D008297:Male; D008970:Molecular Weight; D010743:Phospholipids / Q000097:blood; D011487:Protein Conformation; D012685:Sepharose; D013045:Species Specificity; D014461:Ultracentrifugation
D000596:Amino Acids; D001059:Apoproteins; D002788:Cholesterol Esters; D005230:Fatty Acids, Nonesterified; D005989:Glycerides; D008074:Lipoproteins; D008075:Lipoproteins, HDL; D010743:Phospholipids; D012685:Sepharose; D002784:Cholesterol