Separation of time-defined avian myeloblastosis virus (AMV) using column cultivation of leukaemic myeblasts.
A new technique is described of column cultivation of cells immobilized on Sial glass cullet. The technique ensures a controlled and continuous production of time-defined young virus. During the column cultivation the morphological characteristics of tho immobilized cells remain essentially unchanged.
D001354:Avian Leukosis Virus / Q000302:isolation & purification*; D009189:Avian Myeloblastosis Virus / Q000254:growth & development / Q000302:isolation & purification*; D002478:Cells, Cultured; D013696:Temperature; D013997:Time Factors