Methylated nucleotides block 5'-terminus of vaccinia virus messenger RNA.
Studies on the nature and location of the methylated nucleotides in mRNA synthesized in vitro by vaccinia virus particles revealed an unusual 5'-terminal structure. Evidence that the pyrophosphate group is blocked by 7-methylguanosine and that both 2'-O-methyl-adenosine and 2'-O-methylguanosine occupy penultimate positions was presented. According to this model, the 5'-termini of vaccinia virus mRNAs are: 7MeG-5'ppp-5'GMepNp and 7MeG-5'AMepNp.
D000241:Adenosine / Q000031:analogs & derivatives / Q000378:metabolism*; D000469:Alkaline Phosphatase / Q000378:metabolism; D001483:Base Sequence; D002848:Chromatography, DEAE-Cellulose; D002855:Chromatography, Thin Layer; D011756:Diphosphates / Q000378:metabolism; D004586:Electrophoresis; D006151:Guanosine / Q000031:analogs & derivatives / Q000378:metabolism*; D008745:Methylation; D010761:Phosphorus Radioisotopes; D012333:RNA, Messenger / Q000096:biosynthesis*; D012367:RNA, Viral / Q000096:biosynthesis*; D012436:S-Adenosylmethionine / Q000378:metabolism; D014316:Tritium; D014616:Vaccinia virus / Q000378:metabolism*
D011756:Diphosphates; D010761:Phosphorus Radioisotopes; D012333:RNA, Messenger; D012367:RNA, Viral; D014316:Tritium; D006151:Guanosine; D012436:S-Adenosylmethionine; D000469:Alkaline Phosphatase; D000241:Adenosine