Fluorescence-detected magnetic circular dichroism of fluorescent and nonfluorescent molecules.
We have modified a spectrometer for the measurement of fluorescence-detected magnetic (and natural) circular dichroism (FDMCD). The instrument can be operated either in a direct mode in which the average and polarization-induced differential fluorescent intensities are recorded separately or in a mode which records their ratio. We have measured the FDMCD of tryptophan, which is itself fluorescent. With the aid of an added fluorescent molecule we also measured the FDMCD of Fe(III) cytochrome c, which is nonfluorescent. In both cases, the FDMCD agreed with the conventional transmission-detected magnetic circular dichroism within experimental uncertainty. The FDMCD of both fluorescent and nonfluorescent molecules is potentially a useful technique for investigating optically dense materials and biological molecules in their native environments and systems in which there is inter- or intramolecular energy transfer.
D002942:Circular Dichroism; D003574:Cytochrome c Group; D008280:Magnetics; D008968:Molecular Conformation; D011487:Protein Conformation; D013050:Spectrometry, Fluorescence / Q000295:instrumentation; D013057:Spectrum Analysis; D014364:Tryptophan
D003574:Cytochrome c Group; D014364:Tryptophan