Platelet aggregometry and anti-platelet isoantibodies.
102 sera from polytransfused patients have been screened in platelet aggregometry. Anti-human platelet isoantibodies, tested against 'responsive' human platelets in PRP, give in the aggregometer a decrease in optical density recorded as a sigmoidal curve. In the first step of reaction PF3 availability suggests that the primary action of antibody is to damage the platelet membrane. The second step might be a true aggregation (an ADP-mediated platelet clumping) or a platelet lysis, depending on the type and the potency of antibody, on the type of platelets (normal or thrombasthenic) and on some experimental conditions. These conclusions, confirmed by electron microscopy findings, suggest that aggregometry is a very rapid and simple method of detecting platelet antibodies.
D000241:Adenosine / Q000494:pharmacology; D000244:Adenosine Diphosphate / Q000494:pharmacology; D000263:Adenylate Kinase / Q000032:analysis; D000818:Animals; D000961:Antilymphocyte Serum / Q000494:pharmacology; D001241:Aspirin / Q000494:pharmacology; D001792:Blood Platelets / Q000187:drug effects* / Q000648:ultrastructure; D003994:Bucladesine / Q000494:pharmacology; D006680:HLA Antigens; D006493:Heparin / Q000494:pharmacology; D007106:Immune Sera; D007518:Isoantibodies; D010974:Platelet Aggregation; D010977:Platelet Factor 3 / Q000032:analysis; D010978:Platelet Factor 4 / Q000032:analysis; D011458:Prostaglandins E / Q000494:pharmacology; D012910:Snake Venoms / Q000494:pharmacology; D013917:Thrombin / Q000494:pharmacology
D000961:Antilymphocyte Serum; D006680:HLA Antigens; D007106:Immune Sera; D007518:Isoantibodies; D011458:Prostaglandins E; D012910:Snake Venoms; D010977:Platelet Factor 3; D010978:Platelet Factor 4; D000244:Adenosine Diphosphate; D003994:Bucladesine; D006493:Heparin; D000263:Adenylate Kinase; D013917:Thrombin; D000241:Adenosine; D001241:Aspirin