title: [Use of a synthetic medium for cultivating pathogenic anaerobes].
abstract: It was shown that a synthetic medium suggested by the authors earlier was useful for the growth and toxin formation of Cl. tetani, Cl. botulinum and Cl. perfringens, types B and E. A study of the character of growth and toxinogensis, microscopic examination of morphology of culture cells and results of passages showed the suggested synthetic medium to be of value; a possibility of its application for studying the nutrient requirements and the role of individual components of the nutrient media in the process of growth and toxinogenesis was also demonstrated.
mesh_terms: D001905:Botulinum Toxins / Q000096:biosynthesis; D003014:Clostridium botulinum / Q000254:growth & development* / Q000472:pathogenicity / Q000648:ultrastructure; D003016:Clostridium perfringens / Q000254:growth & development* / Q000472:pathogenicity / Q000648:ultrastructure; D003017:Clostridium tetani / Q000254:growth & development* / Q000472:pathogenicity / Q000648:ultrastructure; D003470:Culture Media; D008854:Microscopy, Electron; D013045:Species Specificity; D013744:Tetanus Toxin / Q000096:biosynthesis; D014118:Toxins, Biological / Q000096:biosynthesis; D014774:Virulence
chemical_list: D003470:Culture Media; D013744:Tetanus Toxin; D014118:Toxins, Biological; D001905:Botulinum Toxins
vernacular_title: Primenenie sinteticheskoĭ sredy dlia kul'tivirovaniia patogennykh anaerobov