Formation of guanine ribonucleotidyl-(3'-5')-adenosine in a flavinogenic strain of Eremothecium ashbyii.
The addition of caffeine caused the accumulation of a new nucleotide compound simultaneously with the rigid inhibition of ribofalvin production in non-growing cells of Eremothecium ashbyii. In the present study we tried to identify the structure of the nucleotide compound using non-growing cells of the mold. 1) It became possible to obtain a large amount of mycelia by masscultivation in a reagent tank. 2) A new nucleotide compound, referred to as compound A in the paper, was extracted with perchloric acid solution and purified by the following subsequent procedures: 1) Dowex 1 x 2 (HCOO-) column, 2) charcoal treatment, 3) DEAE-Sephadex A25 (CI-) column, 4) Dowex 1 x 2 (C1-) column, and 5) DEAE-Sephadex A25 (HCO3-) column. 3) The structure of the new nucleotide compound was proved to be guanine ribonucleotidyl-(3'-5')-adenosine (GpA) from the results of the following analyses: 1) alkaline degradation, 2) UV-spectra, IR-spectra and NMR-spectra, and 3) enzymatic treatments with RNase T2 and phosphodiesterase. 4) The roles of caffeine and guanine ribonucleotidyl-(3'-5')-adenosine in connection with flavinogenesis of this mold were discussed.
D000241:Adenosine / Q000031:analogs & derivatives*; D001203:Ascomycota / Q000378:metabolism*; D002110:Caffeine / Q000494:pharmacology*; D055598:Chemical Phenomena; D002621:Chemistry; D002852:Chromatography, Ion Exchange; D002854:Chromatography, Paper; D003470:Culture Media; D015226:Dinucleoside Phosphates; D006150:Guanine Nucleotides / Q000032:analysis / Q000096:biosynthesis*; D010727:Phosphoric Diester Hydrolases; D012256:Riboflavin / Q000096:biosynthesis*; D012260:Ribonucleases; D004718:Saccharomycetales / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*
D003470:Culture Media; D015226:Dinucleoside Phosphates; D006150:Guanine Nucleotides; D002110:Caffeine; C013011:guanine ribonucleotidyl-(3'-5')-adenosine; D012260:Ribonucleases; D010727:Phosphoric Diester Hydrolases; D000241:Adenosine; D012256:Riboflavin