Preferential synthesis of viral late RNA by nuclei isolated from SV40 lytically infected cells.
Nuclei from SV40-infected monkey cells were isolated late in lytic infection and their cell-free transcriptional activity was characterized. 3H-RNA synthesized in vitro was hybridized to excess quantities of separated SV40 DNA strands which were each covalently bound to Sepharose. It was found that 3-5% of the newly synthesized RNA is virus-specific and that the plus-strand DNA, coding for late RNA sequences, is transcribed at a rate about 15 times higher than that of the minus-strand DNA, which codes for early RNA sequences. This indicates that transcriptional control has a major role in determining the relative abundancy of early and late RNA classes in lytically infected cells.
D000546:Amanitins / Q000494:pharmacology; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism; D003609:Dactinomycin / Q000494:pharmacology; D007700:Kinetics; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000096:biosynthesis*; D013539:Simian virus 40; D013696:Temperature; D014158:Transcription, Genetic; D014500:Uracil Nucleotides / Q000378:metabolism
D000546:Amanitins; D004279:DNA, Viral; D012367:RNA, Viral; D014500:Uracil Nucleotides; D003609:Dactinomycin