The insulin receptor of the turkey erythrocyte. Characterization of the membrane-bound receptor.
The insulin receptor of the turkey erythrocyte has previously been shown to be very similar to that of the mammalian insulin receptors. As a first step in the isolation of this receptor a highly purified plasma membrane fraction has been prepared. The binding characteristics of the purified membrane-bound receptor were identical to those found with intact erythrocytes, but the membrane preparation had very little insulin-degrading activity. Isolation of the membrane by the methods described gave a 100-fold purification of the insulin receptor with 67% yield.
D000818:Animals; D002413:Cations, Divalent; D002462:Cell Membrane / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000378:metabolism*; D007328:Insulin / Q000378:metabolism*; D011485:Protein Binding; D011956:Receptors, Cell Surface; D012964:Sodium / Q000494:pharmacology; D013696:Temperature; D014422:Turkeys
D002413:Cations, Divalent; D007328:Insulin; D011956:Receptors, Cell Surface; D012964:Sodium