Cyclic AMP response of isolated Snell adrenocortical carcinoma 494 cells to trophic hormones and other substances.
Suspensions of viable cells were prepared from solid tumor of the Snell adrenocortical carcinoma 494 without the use of proteolytic enzymes. Cyclic AMP formation in these cells was stimulated by ACTH, LH, FSH and TSH but not by prostaglandins (E1, E2, F1alpha and F2alpha), insulin and secretin. Glucagon tested at a single dose level of 50 mug increased cyclic AMP to about 65% of the maximum amounts obtained with ACTH. When Ca++ was omitted from the incubation medium, the response to ACTH was considerably reduced while that to LH was essentially unchanged. Low concentrations of EGTA (0.3 MM) abolished the ACTH response almost completely but caused only a partial reduction in the response to LH; as much as 10 mM EGTA was required to obtain complete inhibition of the latter.
D000306:Adrenal Cortex Neoplasms / Q000378:metabolism*; D000310:Adrenal Gland Neoplasms / Q000378:metabolism*; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D002460:Cell Line; D000242:Cyclic AMP / Q000378:metabolism*; D004533:Egtazic Acid / Q000494:pharmacology; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D005934:Glucagon / Q000494:pharmacology; D006728:Hormones / Q000494:pharmacology*; D007773:Lactates / Q000378:metabolism; D007986:Luteinizing Hormone / Q000494:pharmacology; D011453:Prostaglandins / Q000494:pharmacology*; D011773:Pyruvates / Q000378:metabolism; D013806:Theophylline / Q000494:pharmacology; D013972:Thyrotropin / Q000494:pharmacology
D006728:Hormones; D007773:Lactates; D011453:Prostaglandins; D011773:Pyruvates; D004533:Egtazic Acid; D000324:Adrenocorticotropic Hormone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D013972:Thyrotropin; D005934:Glucagon; D013806:Theophylline; D000242:Cyclic AMP; D002118:Calcium