Induction of increased calcium uptake in mouse T lymphocytes by concanavalin A and its modulation by cyclic nucleotides.
The binding of concanavqlin A to T but not B mouse spleen lymphocytes increases Ca-2+ uptake in these cells which is measurable by 45 s and complete by 1 min. Dibutyrl cyclic AMP, but not sodium azide inhibits induced Ca-2+ uptake, wheras dibutyryl cyclic GMP enhances it. B cell mitogens do not cause a similar Ca-2+ uptake in mouse B lymphocytes. The induction of increased Ca-2+ uptake by T cells is discussed in terms of gated membrane channels for Ca-2+.
D000818:Animals; D001386:Azides / Q000494:pharmacology; D001402:B-Lymphocytes / Q000378:metabolism; D003994:Bucladesine / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002250:Carbon Radioisotopes; D002455:Cell Division / Q000187:drug effects; D002463:Cell Membrane Permeability / Q000187:drug effects; D003208:Concanavalin A / Q000494:pharmacology*; D000242:Cyclic AMP / Q000494:pharmacology*; D006152:Cyclic GMP / Q000031:analogs & derivatives / Q000494:pharmacology*; D003600:Cytosol / Q000378:metabolism; D003864:Depression, Chemical; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008934:Mitogens / Q000494:pharmacology; D013154:Spleen; D013268:Stimulation, Chemical; D013601:T-Lymphocytes / Q000378:metabolism*
D001386:Azides; D002250:Carbon Radioisotopes; D008934:Mitogens; D003208:Concanavalin A; D003994:Bucladesine; D000242:Cyclic AMP; D006152:Cyclic GMP; D002118:Calcium