title: Kinetics of utilization of Sendai virus RNA and protein in the process of virion assembly.
abstract: The synthesis of the 50S genomic RNA and strucural proteins of Sendai virus was examined with respect to their utilization in virus assembly. It was found that during a single cycle of infection, 50S RNA was synthesized before the structural proteins and that both RNA and protein were synthesized 2 to 4 h before their appearance in released virions. Pulse-chase labeling indicated that the NP and P proteins synthesized early and the M and F proteins synthesized late were preferentially incorporated into virus relative to the other viral proteins. The kinetics of incorporation of pulse-labeled NP protein suggested that it was withdrawn from a relatively large pool whereas the M protein appeared to be present in a relatively small pool in the cytoplasm. Further, it was possible to chase pulse-labeled M protein, but not NP protein, from the cell during an 8-h time period.
mesh_terms: D000818:Animals; D002417:Cattle; D002460:Cell Line; D009024:Morphogenesis; D010222:Parainfluenza Virus 1, Human / Q000254:growth & development / Q000378:metabolism*; D012367:RNA, Viral / Q000096:biosynthesis*; D014779:Virus Replication
chemical_list: D012367:RNA, Viral