title: Epithelial cell cultures from normal and cancerous human tissues.
abstract: Thirty epithelial cell strains were isolated from human carcinomas and normal epithelial tissues by collagenase digestion and selective removal of fibroblasts with trypsin-Versene. Most strains were obtained from metastatic carcinomas or epithelia of the urinary and intestinal tracts. The success rate for growth of both neoplastic and normal tissues (excluding skin) was 38%. Six of these strains showed gross morphologic and chromosome changes typical of malignant cells. Nine resembled normal epithelium. The other 15 exhibited some degree of morphologic change from normal.
mesh_terms: D002455:Cell Division; D002469:Cell Separation; D002478:Cells, Cultured; D004847:Epithelial Cells; D004848:Epithelium; D006801:Humans; D003012:Microbial Collagenase; D009369:Neoplasms / Q000473:pathology; D014357:Trypsin
chemical_list: D014357:Trypsin; D003012:Microbial Collagenase