Estrogen receptor in rat liver and its dependence on prolactin.
Estrogen receptor is shown to be present in the livers of adult rats. The receptor binds estradiol-17beta with a Kd of 1 x 10(-10) M and sediments at 8 S in sucrose gradients. Other estrogens and anti-estrogens compete for estradiol binding, while nonestrogenic steroids do not. Receptor levels fall dramatically after hypophysectomy, but can be partially restored within 18 hours by a single injection of prolactin. It is known that prolactin critically regulates the level of its own receptor in the liver, and we now suggest that it also exerts a primary control over the availability of liver estrogen receptor.
D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive / Q000187:drug effects; D002499:Centrifugation, Density Gradient; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D004031:Diestrus; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D007016:Hypophysectomy; D007700:Kinetics; D008099:Liver / Q000378:metabolism* / Q000648:ultrastructure; D010902:Pituitary Gland / Q000502:physiology; D011247:Pregnancy; D011388:Prolactin / Q000494:pharmacology*; D051381:Rats; D011956:Receptors, Cell Surface / Q000187:drug effects*
D011956:Receptors, Cell Surface; D004958:Estradiol; D011388:Prolactin