title: [On the suitability of migration inhibition techniques in the in-vitro-diagnostics of chromium allergy (author's transl)].
abstract: 1. Under the conditions of cell culture potassium dichromate is reduced to trivalent chromium (diphenylcarbacid/fotometry). Trivalent chromium reacts with proteins more strongly than the hexavalent chromium (gelchromatography/atom absorption spectrophotometry) and presumably represents the actual hapten. 2. Because of this in-vitro-conjugation chromium salts are suitable in their unconjugated form for applying in migration inhibition tests (capillary/Clausen technique). 3. In migration inhibition tests potassium dichromate showed a better antigenicity than chromium chloride. The capillary method was more sensitive than the Clausen technique when using the same test concentration. 4. Correlations between the degree of the patch test and the value of the migration inhibition did not exist.
mesh_terms: D000485:Allergens; D002464:Cell Migration Inhibition; D002857:Chromium / Q000276:immunology; D004160:Diphenylcarbazide; D006241:Haptens; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis*; D012709:Serum Albumin; D012882:Skin Tests
chemical_list: D000485:Allergens; D006241:Haptens; D012709:Serum Albumin; D002857:Chromium; D004160:Diphenylcarbazide
vernacular_title: Zur Eignugn von Migrationshemmtechniken für die In-vitro-Diagnostik der Chrom-Allergie.