Size fractionation of DNA fragments by liquid-liquid chromatography.
A method for the fractionation of double-stranded DNA fragments from 150 to 22000 b.p. in size by liquid-liquid chromatography is described. The procedure makes use of the fact that the partitioning of DNA in a polyethylene glycol-dextran system is size dependent and can be altered by alkali metal cations. Cellulose or celite are used as supports for the stationary, dextran-rich phase. Examples show the fractionation of digests of T7 DNA produced by Dpn II and Hind II restriction endonulceases as well as lambda DNA digests produced by Hind III and Eco RI restriction endonucleases.
D010582:Bacteriophage lambda / Q000737:chemistry*; D002845:Chromatography / Q000379:methods; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000302:isolation & purification; D004279:DNA, Viral / Q000302:isolation & purification; D003911:Dextrans; D004926:Escherichia coli / Q000737:chemistry*; D007202:Indicators and Reagents; D007763:Lac Operon; D008970:Molecular Weight; D011092:Polyethylene Glycols
D004269:DNA, Bacterial; D004279:DNA, Viral; D003911:Dextrans; D007202:Indicators and Reagents; D011092:Polyethylene Glycols; D004262:DNA Restriction Enzymes