Attempts to detect virus-specific DNA sequences in human tumors. III. Epstein-Barr viral DNA in non-lymphoid nasopharyngeal carcinoma cells.
Fourteen tumors of the nasopharyngeal region were analyzed for the presence of Epstein-Barr virus-specific DNA by DNA-cRNA hybridization. These data were compared to the histology of the respective tumors and the seroreactivity of the tumor-bearing patient against EBV-related antigens. With one exception, all tumor pieces containing nasopharyngeal carcinoma cells hybridized significantly with EBV-cRNA. Tumors of predominantly epithelial morphology annealed in the highest range. In situ-hybridization of freeze sections from a tumor containing almost equal amounts of tumor cells and lymphocytes revealed hybridizing DNA within nuclei of non-lymphoid cells. Although these data do not exclude the presence of EBV-DNA within lymphoid cells, they clearly demonstrate that in nasopharyngeal carcinomas the vast majority of EBV-specific DNA rests within non-lymphoid cells.
D002277:Carcinoma / Q000382:microbiology*; D002294:Carcinoma, Squamous Cell / Q000382:microbiology; D004273:DNA, Neoplasm / Q000302:isolation & purification; D004279:DNA, Viral / Q000302:isolation & purification*; D004854:Herpesvirus 4, Human / Q000302:isolation & purification*; D006801:Humans; D006824:Hybridization, Genetic; D009303:Nasopharyngeal Neoplasms / Q000382:microbiology* / Q000473:pathology; D009693:Nucleic Acid Hybridization; D012334:RNA, Neoplasm / Q000302:isolation & purification
D004273:DNA, Neoplasm; D004279:DNA, Viral; D012334:RNA, Neoplasm