Affinity chromatography of thiamin pyrophosphokinase of rat brain.
Affinity column chromatography coupled with thiamin monophosphate absorbs thiamin pyrophosphokinase activity in the crude extract of rat brain, and the enzyme can be eluted from the column by 0.01 mM thiamin with approximately 700-fold purification.
D000818:Animals; D001921:Brain / Q000201:enzymology*; D002846:Chromatography, Affinity; D008297:Male; D010770:Phosphotransferases / Q000302:isolation & purification*; D051381:Rats; D012685:Sepharose; D013831:Thiamine
D012685:Sepharose; D010770:Phosphotransferases; D013831:Thiamine