[3H-thymidine-paper strip-counting of in vitro lymphocyte transformation. II. Specific antigens in the whole blood assay (author's transl)].
Erythrocytes did not influence the harvesting procedure or the paper strip counting method in a liquid scintillation counter using the whole blood assay of lymphocyte transformation test. The leucocyte-erythrocyte-ratio influences the blastogenesis in vitro. Only 1--2 ml of peripheral blood are necessary to proof lymphocyte reactivity to mitogens or antigens. The storage of the blood is possible over a period of 24 hours (4 degrees C) if the absolute lymphocytes count remains constant.
D000293:Adolescent; D000328:Adult; D000368:Aged; D000946:Antigens, Fungal; D000956:Antigens, Viral; D002176:Candida albicans / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D004912:Erythrocytes / Q000276:immunology; D006801:Humans; D007223:Infant; D008213:Lymphocyte Activation; D008875:Middle Aged; D008934:Mitogens; D009109:Mumps virus / Q000276:immunology; D010209:Paper; D013936:Thymidine / Q000378:metabolism; D014373:Tuberculin
D000946:Antigens, Fungal; D000956:Antigens, Viral; D008934:Mitogens; D014373:Tuberculin; D013936:Thymidine
Die 3H-Thymidin-Papierplättchen-Modifikation des lymphozytentransfomationstestes (LTT). II. Vollblut-LTT mit Einsatz spezifischer Antigene.