Differentiation between steroid hormone receptors CBG and SHBG in human target organ extracts by a single-step assay.
The steroid complexes of (plasma) corticosteroid-binding globulin can be distinguished from intracellular steroid-receptor complexes by agar electrophoresis at low temperature in neuraminidase-treated tissue extracts. With this method, the presence of progesterone receptor has been demonstrated in heavily plasma-protein-contaminated human uterus "cytosol", but not in human mammary carcinoma extracts. SHBG and "basic" receptors for estradiol and dihydrotestosterone in human uterus cytosol could also be assayed simultaneously.
D000305:Adrenal Cortex Hormones / Q000378:metabolism*; D000328:Adult; D000368:Aged; D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D001943:Breast Neoplasms / Q000378:metabolism*; D002417:Cattle; D003600:Cytosol / Q000378:metabolism; D013196:Dihydrotestosterone / Q000378:metabolism*; D005260:Female; D006801:Humans; D006854:Hydrocortisone / Q000378:metabolism*; D008875:Middle Aged; D009124:Muscle Proteins / Q000378:metabolism; D009363:Neoplasm Proteins / Q000378:metabolism; D009439:Neuraminidase; D011247:Pregnancy; D011374:Progesterone / Q000378:metabolism*; D011485:Protein Binding; D011956:Receptors, Cell Surface; D012794:Sialic Acids / Q000378:metabolism; D014599:Uterus / Q000378:metabolism*
D000305:Adrenal Cortex Hormones; D009124:Muscle Proteins; D009363:Neoplasm Proteins; D011956:Receptors, Cell Surface; D012794:Sialic Acids; D013196:Dihydrotestosterone; D011374:Progesterone; D009439:Neuraminidase; D006854:Hydrocortisone