5alpha-Dihydrotestosterone binding protein in rat ventral prostate; purification, nuclear incorporation, and subnuclear localization.
Treatment of cytosol from the rat ventral prostate with cold acetone (-20 degrees C) evoked a 8 approximately 10-fold increase in the binding capacity with 5alpha-dihydrotestosterone (DHT). Starting from the extract of acetone-dried prostate cytosol, some 400 approximately 600-fold purification of the DHT-binding protein complex was acieved by (NH4)2504 fractionation, DEAE-cellulose chromatography and gel- filtration with Sephadex G-200. The purified 3H-DHT-binding protein complex was incorporated into the nuclei from the ventral prostate in a temperature dependent manner. The similar incorporation was also observed in nuclei from the liver and the kidney...
D000818:Animals; D003600:Cytosol / Q000378:metabolism; D013196:Dihydrotestosterone / Q000302:isolation & purification / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D011467:Prostate / Q000378:metabolism*; D051381:Rats; D011956:Receptors, Cell Surface
D011956:Receptors, Cell Surface; D013196:Dihydrotestosterone