The enhancing effect of copper, nickel, and cobalt ions on plaque formation by Semliki Forest virus (SFV) in chicken embryo fibroblasts.
In cultured chicken embryo fibroblasts treated with copper-(copper cation 10-minus 3.5-10-minus 5 M), nickel and cobalt salts an enhancement of the plating efficiency of Semliki Forest virus could be observed. This augmented plaque formation was most probably due to a higher adsorption rate of virions to the cell surface under the influence of the transition metal ions. The plating efficiency of West Nile virus in chicken-embryo fibroblasts and, to a lesser degree, of poliovius type 1 and 2 in KB-cells was also enhanced by copper sulfate.
D000327:Adsorption; D000818:Animals; D002642:Chick Embryo; D003035:Cobalt / Q000494:pharmacology*; D003300:Copper / Q000494:pharmacology*; D046508:Culture Techniques; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D005347:Fibroblasts; D009532:Nickel / Q000494:pharmacology*; D017955:Poliovirus / Q000187:drug effects / Q000254:growth & development; D011691:Puromycin / Q000494:pharmacology; D012672:Semliki forest virus / Q000187:drug effects / Q000254:growth & development*; D013268:Stimulation, Chemical; D013696:Temperature; D010948:Viral Plaque Assay; D014779:Virus Replication; D014902:West Nile virus / Q000187:drug effects / Q000254:growth & development; D010135:p-Fluorophenylalanine / Q000494:pharmacology
D003609:Dactinomycin; D003035:Cobalt; D011691:Puromycin; D010135:p-Fluorophenylalanine; D003300:Copper; D009532:Nickel; D003513:Cycloheximide