The kinetics of interferon production by mouse lymphocytes and its modulating effect of the virus plaqueforming cell assay as a quantitative method to determine activated lymphocytes.
The validity of the virus plaque-forming cell (V-PFC) assay as a means to quantitate stimulated mouse T cells is dependent on the rate and amount of coinciding interferon production which varies with different strains of mice. In addition, the V-PFC assay demonstrates that the kinetics of cellular activation by mitogens initially involves about 0.01% of responding cells and possibly recruitment of other cells which include cells requiring a longer lap period for activation.
D000818:Animals; D003208:Concanavalin A; D007372:Interferons / Q000096:biosynthesis*; D007700:Kinetics; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D013154:Spleen / Q000276:immunology; D013936:Thymidine / Q000378:metabolism; D014721:Vesicular stomatitis Indiana virus / Q000254:growth & development; D010948:Viral Plaque Assay
D003208:Concanavalin A; D007372:Interferons; D013936:Thymidine