Chromatographic-fluorometric analysis of antileprotic sulfones.
Modifications to the power supply system of a spectrophotofluorometer are described. These modifications stabilize the output of the xenon are lamp and permit the determination of nanogram quantities of antileprotic sulfones. The sulfones are removed from plasma by a single extraction with ethyl acetate, then separated by high-pressure liquid chromatography on silica, and detected in the effluent by their fluorescence. The method is specific, rapid, and reproducible.
D001322:Autoanalysis; D002845:Chromatography; D003622:Dapsone / Q000031:analogs & derivatives / Q000032:analysis*; D007917:Leprostatic Agents / Q000032:analysis*; D008722:Methods; D008832:Microchemistry; D011312:Pressure; D012822:Silicon Dioxide; D013050:Spectrometry, Fluorescence; D013450:Sulfones / Q000032:analysis*
D007917:Leprostatic Agents; D013450:Sulfones; D012822:Silicon Dioxide; D003622:Dapsone