Resolution and functional characterization of two mitochondrial iron-sulphur centres of the 'high-potential iron-sulphur protein' type.
Two distinct iron-sulphur centres of the 'HiPIP' (high-potential iron-protein) type are distinguished in both pigeon heart and ox heart mitochondria. These two species, although both are paramagnetic in the oxidized state, exhibit signals which differ in their detailed line shape, field position, and temperature- and power-dependence. They also exhibit different thermodynamic and kinetic behaviour and are located on opposite sides of the mitochondrial coupling membrane. One of these centres corresponds to Centre S-3. The other 'HiPIP'-type centre is removed readily from the mitochondrial membrane and its physiological function is not known.
D000818:Animals; D000968:Antimycin A / Q000494:pharmacology; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D002417:Cattle; D010856:Columbidae; D004578:Electron Spin Resonance Spectroscopy; D005292:Ferricyanides; D005971:Glutamates; D006321:Heart / Q000187:drug effects; D007501:Iron / Q000032:analysis*; D007700:Kinetics; D008293:Malates; D008928:Mitochondria / Q000032:analysis* / Q000187:drug effects; D009206:Myocardium / Q000032:analysis; D010084:Oxidation-Reduction; D010618:Phenanthrolines / Q000494:pharmacology; D013455:Sulfur / Q000032:analysis*; D013696:Temperature; D013816:Thermodynamics
D005292:Ferricyanides; D005971:Glutamates; D008293:Malates; D010618:Phenanthrolines; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D000968:Antimycin A; D013455:Sulfur; D007501:Iron