The estimation of 3,4-dihydroxyphenylacetic acid, homovanillic acid and homo-isovanillic acid in nervous tissue by gas-liquid chromatography and electron capture detection.
1 A gas chromatographic method using electron capture detection is described for the estimation of three acidic metabolites of dopamine, 4-hydroxy-3-methoxyphenylacetic acid (homovanillic acid, HVA), 3,4-dihydroxyphenylacetic acid (DOPAC) and 3-hydroxy-4-methoxyphenylacetic acid (homo-isovanillic acid, iso-HVA). The method is based on the formation of the trifluoroacetyl-hexafluoroisopropyl derivatives of the three acids. 2 The method has been applied to the estimation of DOPAC, HVA and iso-HVA in tissues from the central and peripheral nervous systems.
D015102:3,4-Dihydroxyphenylacetic Acid / Q000032:analysis; D000818:Animals; D001923:Brain Chemistry; D002849:Chromatography, Gas; D004578:Electron Spin Resonance Spectroscopy; D005123:Eye / Q000032:analysis; D006719:Homovanillic Acid / Q000032:analysis* / Q000134:cerebrospinal fluid; D007536:Isomerism; D051379:Mice; D009417:Nerve Tissue / Q000032:analysis*; D010648:Phenylacetates / Q000032:analysis* / Q000134:cerebrospinal fluid; D051381:Rats
D010648:Phenylacetates; D015102:3,4-Dihydroxyphenylacetic Acid; D006719:Homovanillic Acid