Pathways of carbohydrate metabolism in mycobacterium tuberculosis H37Rv1.
Radiorespirometric studies using glucose labelled at 1, 2, 3-4, and 6 positions and enzymatic studies were conducted to determine the primary pathways of glucose dissimilation in Mycobacterium tuberculosis H37Rv. The pattern of 14CO2 recovery was C3-4 greater than C1 greater than C6 = C2. The Embden-Meyerhof pathway was found to be the predominant pathway for glucose oxidation, operative to the extent of 94%. The pentose phosphate pathway accounted for the remaining 6%. Maximum incorporation of 14C into cellular components was from C2 and C6 labelled glucose.
D050260:Carbohydrate Metabolism; D002245:Carbon Dioxide / Q000096:biosynthesis; D002474:Cell-Free System; D006597:Fructose-Bisphosphatase / Q000378:metabolism; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism; D005941:Glucokinase / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D009169:Mycobacterium tuberculosis / Q000201:enzymology / Q000378:metabolism*; D010084:Oxidation-Reduction; D010732:Phosphofructokinase-1 / Q000378:metabolism; D010734:Phosphogluconate Dehydrogenase / Q000378:metabolism; D011770:Pyruvate Kinase / Q000378:metabolism
D002245:Carbon Dioxide; D010734:Phosphogluconate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D010732:Phosphofructokinase-1; D005941:Glucokinase; D011770:Pyruvate Kinase; D006597:Fructose-Bisphosphatase; D005634:Fructose-Bisphosphate Aldolase; D005947:Glucose