title: Preparation and spread of unfixed metaphase chromosomes for immunofluorescence staining of nuclear antigens.
mesh_terms: D000818:Animals; D000941:Antigens / Q000032:analysis*; D000956:Antigens, Viral / Q000032:analysis; D001324:Autoantigens / Q000032:analysis; D002051:Burkitt Lymphoma; D002460:Cell Line; D002467:Cell Nucleus / Q000276:immunology*; D002471:Cell Transformation, Neoplastic; D002498:Centrifugation; D002875:Chromosomes / Q000276:immunology*; D003078:Colchicine / Q000494:pharmacology; D003584:Cytological Techniques; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells; D004854:Herpesvirus 4, Human / Q000276:immunology; D006649:Histocompatibility Antigens / Q000032:analysis; D006801:Humans; D007739:L Cells; D008297:Male; D008441:Maxillary Neoplasms; D051379:Mice; D008938:Mitosis; D013539:Simian virus 40 / Q000276:immunology
chemical_list: D000941:Antigens; D000956:Antigens, Viral; D001324:Autoantigens; D006649:Histocompatibility Antigens; D003078:Colchicine