title: A precise chromatography system for specific technetium-99m radiopharmaceuticals.
abstract: New chromatographic procedures are needed to accurately determine the radiochemical purity of technetium-99m labeled radiopharmaceuticals. A chromatography system has been developed for technetium-99m diethylenetriaminepentacetic acid, technetium-99m diphosphonate, and technetium-99m pyrophosphate. The technique involves spotting the radiopharmaceuticals on instant thin layer chromatography-silica gel and developing with acetone (solvent from migrates approximately 12 cm from the origin), air drying, and redeveloping the strip in normal saline (solvent front migrates approximately 6 cm from the origin). The procedure is simple to perform, rapid, accurate, and clearly separates technetium-99m pertechnetate, the technetium-99m radiopharmaceutical, and hydrolyzed reduced technetium-99m. It can be applied in most nuclear medicine laboratories and has the potential to predict the quality of radionuclide images obtained.
mesh_terms: D002855:Chromatography, Thin Layer / Q000379:methods*; D011756:Diphosphates / Q000032:analysis; D006868:Hydrolysis; D007553:Isotope Labeling; D009943:Organophosphorus Compounds / Q000032:analysis; D010084:Oxidation-Reduction; D004369:Pentetic Acid / Q000032:analysis; D012822:Silicon Dioxide; D013667:Technetium / Q000032:analysis* / Q000592:standards
chemical_list: D011756:Diphosphates; D009943:Organophosphorus Compounds; D013667:Technetium; D012822:Silicon Dioxide; D004369:Pentetic Acid