Proportional activities of glycerol kinase and glycerol 3-phosphate dehydrogenase in rat hepatomas.
The activities of glycerol 3-phosphate dehydrogenase (EC 1.1.1.8), glycerol kinase (EC 2.7.1.30), lactate dehydrogenase (EC 1.1.1.27), "malic' enzyme (L-malate-NADP+ oxidoreductase; EC 1.1.1.40) and the beta-oxoacyl-(acyl-carrier protein) reductase component of the fatty acid synthetase complex were measured in nine hepatoma lines (8 in rats, 1 in mouse) and in the livers of host animals. With the single exception of Morris hepatoma 16, which had unusually high glycerol 3-phosphate dehydrogenase activity, the activities of glycerol 3-phosphate dehydrogenase and glycerol kinase were highly correlated in normal livers and hepatomas (r = 0.97; P less than 0.01). The activities of these two enzymes were not strongly correlated with the activities of any of the other three enzymes. The primary function of hepatic glycerol 3-phosphate dehydrogenase appears to be in gluconeogenesis from glycerol.
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000201:enzymology*; D002460:Cell Line; D064429:Fatty Acid Synthases / Q000378:metabolism; D005991:Glycerol Kinase / Q000378:metabolism*; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism*; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D008113:Liver Neoplasms / Q000201:enzymology*; D009374:Neoplasms, Experimental / Q000201:enzymology; D010770:Phosphotransferases / Q000378:metabolism*; D051381:Rats
D005993:Glycerolphosphate Dehydrogenase; D007770:L-Lactate Dehydrogenase; D064429:Fatty Acid Synthases; D010770:Phosphotransferases; D005991:Glycerol Kinase