Polypeptides isolated from ribosome-like structures occluded in avian myeloblastosis virus (AMV).
The protein composititon of ribosome-like particles isolated from AMV was determined by acrylamide gel electrophoresis and by immunological methods. It was established that the protein spectrum of ribosome-like particles differed significantly form the total protein spectrum of AMV. The most characteristic protein components of ribosome-like particles had a molecular weight in the range of 70 000--110 000. Apart from these proteins, the viral ribosomal particles contained  a small amount of proteins with a molecular weight of 14 000--35 000 that could not be removed even by extensive purification. Immunological studies of the proteins of ribosome-like particles revealed the presence of antigenic determinants of ribosomal proteins. Furthermore, throughout the purification procedure the material contained components that reacted with antibodies against gs antigens.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D001353:Avian Leukosis / Q000032:analysis; D001354:Avian Leukosis Virus / Q000032:analysis*; D009189:Avian Myeloblastosis Virus / Q000032:analysis* / Q000276:immunology; D002645:Chickens; D010455:Peptides / Q000302:isolation & purification*; D012270:Ribosomes / Q000032:analysis*; D014764:Viral Proteins / Q000302:isolation & purification
D000956:Antigens, Viral; D010455:Peptides; D014764:Viral Proteins