title: Interaction of lysine-rich histone and DNA in chromatin structure.
abstract: The heat denaturation and renaturation curves of rat liver and ascites hepatoma (AH 108A) chromatins were measured. In these renaturation curves, there are small sigmoidal regions. These sigmoidal regions remained in redenaturation curves and were largely stable to DNAase I digestion. When the chromatins were treated stepwise with NaClO4 and lysine-rich histones were removed, the sigmoidal regions in the renaturation curves disappeared. These results suggested that the sigmoidal regions reflected the interaction of DNA and lysine-rich histones.
mesh_terms: D001665:Binding Sites; D006528:Carcinoma, Hepatocellular / Q000032:analysis; D002843:Chromatin / Q000032:analysis*; D004247:DNA / Q000032:analysis; D003851:Deoxyribonucleases; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000032:analysis; D007700:Kinetics; D008099:Liver / Q000032:analysis; D008113:Liver Neoplasms; D008239:Lysine; D046911:Macromolecular Substances; D009374:Neoplasms, Experimental / Q000032:analysis; D009691:Nucleic Acid Denaturation; D009695:Nucleic Acid Renaturation; D011485:Protein Binding; D011489:Protein Denaturation; D013696:Temperature
chemical_list: D002843:Chromatin; D006657:Histones; D046911:Macromolecular Substances; D004247:DNA; D003851:Deoxyribonucleases; D008239:Lysine