An estimate of the minimal frequency of cytolytic T lymphocyte effector cells generated in allogeneic reactions.
A 'micro' modification of the standard 51Cr release assay was developed. With this assay, significant cytolysis was obtained employing 100--200 51Cr-labeled target cells and fewer than 100 lymphocytes obtained from mixed leukocyte cultures. A minimal estimate as to the actual frequency of effector cells was determined for both primary (1 cell in 30) and secondary (1 cells in 8) cytolytic T lymphocyte populations.
D000818:Animals; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology*