title: Analysis of defective SV40 DNA by agarose gel electrophoresis.
abstract: The SV40 DNA that was generated by undiluted passaging of the virus was analysed by agarose gel electrophoresis. Nine bands of virus DNA were distinguished and each band contained a specific size class of DNA, all shorter than the complete SV40 genome as was determined by electron microscopy measurements. A difference of 2% in length, about 100 base pairs, resulted in a clear band splitting. Two sets of undiluted passaging were established and the defective DNA in the two sets had both different and similar size classes varying in length from 96% to 73% of the unit length SV40 DNA.
mesh_terms: D002460:Cell Line; D004279:DNA, Viral / Q000032:analysis*; D003673:Defective Viruses / Q000032:analysis* / Q000254:growth & development; D004587:Electrophoresis, Agar Gel; D008970:Molecular Weight; D013539:Simian virus 40 / Q000032:analysis* / Q000254:growth & development
chemical_list: D004279:DNA, Viral