Establishment of replicating long-term lines of rabbit macrophages and lymphocytes.
Macrophages (monocyte derived) and lymphocytes have been successfully cultured from rabbit peripheral blood so that replicating long-term cultures are maintained both in media and in the viable, frozen state. The macrophages are considered as such on the basis of phagocytic capabilities, gamma-globulin receptor sites as evidenced by rosette formation with sensitized red cells and disparate enzyme content from the lymphocytes.
D000135:Acid Phosphatase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000626:Aminopeptidases / Q000097:blood; D000818:Animals; D001666:Binding Sites, Antibody; D002448:Cell Adhesion; D002460:Cell Line; D002469:Cell Separation; D046508:Culture Techniques / Q000379:methods*; D003576:Electron Transport Complex IV / Q000097:blood; D004912:Erythrocytes / Q000276:immunology; D005455:Fluorescent Antibody Technique; D005615:Freezing; D005696:Galactosidases / Q000097:blood; D006596:Hexosaminidases / Q000097:blood; D007104:Immune Adherence Reaction; D008214:Lymphocytes / Q000201:enzymology / Q000276:immunology; D008264:Macrophages / Q000201:enzymology / Q000276:immunology; D008854:Microscopy, Electron; D010447:Peptide Hydrolases / Q000097:blood; D010587:Phagocytosis; D010727:Phosphoric Diester Hydrolases / Q000097:blood; D011817:Rabbits; D013385:Succinate Dehydrogenase / Q000097:blood
D013385:Succinate Dehydrogenase; D003576:Electron Transport Complex IV; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D010727:Phosphoric Diester Hydrolases; D005696:Galactosidases; D006596:Hexosaminidases; D010447:Peptide Hydrolases; D000626:Aminopeptidases