Galactose-6-sulfatase from Actinobacillus sp. IFO-13310 and its action on sulfated oligosaccharides from keratan sulfate.
A 6-sulfatase specific for sugasr of the galactose configuration was purified 81-fold from the crude extract of Actinobacillus sp. IFO-13310. This preparation contained activity towards both N-acetylgalactosamine 6-sulfate and galactose 6-sulfate (relative activity, 2.4 : 1). The enzyme also release inorganic sulfate from the non-reducing galactose 6-sulfate end group of a trisaccharide disulfate prepared from keratan sulfate by sequential degradation with endo-beta-galactosidase, N-acetylglucosamine-6-sulfatase and exo-beta-N-acetylglucosaminidase. In addition, a tetrasaccharide trisulfate bearing the non-reducing N-acetylglucosamine 6-sulfate end group, also enzymatically prepared from keratan sulfate, was degraded to give rise to inorganic sulfate, N-acetylglucosamine and galactose by the sequential action of this enzyme, N-acetylglucosamine-6-sulfatase, exo-beta-N-acetylglucosaminidase and exo-beta-galactosidase (Charonia lampas).
D000116:Acetylgalactosamine; D000117:Acetylglucosamine; D000118:Acetylglucosaminidase / Q000378:metabolism; D000188:Actinobacillus / Q000201:enzymology*; D005690:Galactose; D005696:Galactosidases / Q000378:metabolism; D006025:Glycosaminoglycans / Q000378:metabolism*; D007632:Keratan Sulfate / Q000378:metabolism*; D008974:Mollusca / Q000201:enzymology; D009844:Oligosaccharides / Q000378:metabolism*; D013379:Substrate Specificity; D013429:Sulfatases / Q000302:isolation & purification / Q000378:metabolism*
D006025:Glycosaminoglycans; D009844:Oligosaccharides; D007632:Keratan Sulfate; D013429:Sulfatases; C018719:N-acetylglucosamine-6-sulfatase; D005696:Galactosidases; D000118:Acetylglucosaminidase; D000116:Acetylgalactosamine; D000117:Acetylglucosamine; D005690:Galactose