Effect of neocarzinostatin on the nucleus, kinetoplast and microtubules of Trypanosoma gambiense and Trypanosoma evansi.
The antibiotic neocarzinostatin (NCS) induces the anucleate form, not the dyskinetoplastic form, of Trypanosoma gambiense and Trypanosoma evansi. Light and electron microscopic studies indicated that production of the anucleate form is due to delay or inhibition of nuclear division. Excess pellicular microtubules are formed after treatment of trypanosomes with NCS, suggesting that in trypanosomes the microtubules replicate by induction, not by division. NCS also causes deformation of the axonemal and spindle microtubules. The K clone of T. evansi is more sensitive than the AK clone to the effects of NCS in inhibiting nuclear division and inducing the anucleate form.
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects*; D002467:Cell Nucleus / Q000187:drug effects; D008870:Microtubules / Q000187:drug effects*; D008938:Mitosis / Q000187:drug effects*; D009940:Organoids / Q000187:drug effects; D014345:Trypanosoma / Q000187:drug effects* / Q000648:ultrastructure; D014347:Trypanosoma brucei gambiense / Q000187:drug effects / Q000648:ultrastructure; D009353:Zinostatin / Q000494:pharmacology*
D000903:Antibiotics, Antineoplastic; D009353:Zinostatin