Early alteration of poliovirus in infected cells and its specific inhibition.
HeLa cells infected with radioactive poliovirus type 2 were disrupted with ultrasonic treatment, followed by addition of a non-ionic detergent. Two types of virus particles were found to sediment at 80 to 90% the rate of native virus. The first of these appeared to be a complex of native virus particles and membrane components, since treatment with 0-2% SDS released infectious native particles. The second was non-infectious and its sedimentation rate was not greatly altered by SDS. One hour after infection this non-infectious particle was the major product of cell-mediated eclipse. We have confirmed that 10 to 30 mg-g/ml S-7, a substituted thiopyrimidine, blocks infection of cells by poliovirus in a specific manner. Analysis of cells infected with radioactive poliovirus type 2 in the presence of S-7 showed that the virus particles remained as the complex which can be disrupted with SDS. In addition to blocking cell-mediated eclipse, S-7 stabilizes poliovirus against heat inactivation in vitro at the same concentrations which block infection. This action resembles the effect of 10-2 M-glutathione, which is also known to block cell-mediated eclipse of poliovirus.
D000327:Adsorption; D002250:Carbon Radioisotopes; D002264:Carboxylic Acids / Q000494:pharmacology; D002499:Centrifugation, Density Gradient; D004591:Electrophoresis, Polyacrylamide Gel; D004686:Encephalomyocarditis virus / Q000254:growth & development; D005978:Glutathione / Q000494:pharmacology; D006367:HeLa Cells; D006358:Hot Temperature; D006801:Humans; D010455:Peptides / Q000032:analysis; D017955:Poliovirus / Q000032:analysis* / Q000187:drug effects / Q000254:growth & development; D011743:Pyrimidines / Q000494:pharmacology; D012229:Rhinovirus / Q000254:growth & development; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013010:Sonication; D013440:Sulfides / Q000494:pharmacology; D014764:Viral Proteins / Q000032:analysis*; D014779:Virus Replication / Q000187:drug effects
D002250:Carbon Radioisotopes; D002264:Carboxylic Acids; D010455:Peptides; D011743:Pyrimidines; D013440:Sulfides; D014764:Viral Proteins; D012967:Sodium Dodecyl Sulfate; D005978:Glutathione