title: Inhibition of bacteriophage PBS2 replication in Bacillus subtilis by phleomycin.
abstract: Phleomycin is an effective inhibitor of the replication of Bacillus subtilis bacteriophage PBS2, whose DNA contains uracil instead of thymine. Phleomycin does not affect the induction of the known phage enzymes involved in deoxyribonucleotide metabolism. But phage DNA synthesis is severely inhibited by phleomycin, and late virion protein synthesis is eliminated. These effects appear to result from a phleomycin-induced degradation of the parental phage DNA. Similar inhibitory and degradative effects on DNA are seen in phleomyinc-treated, uninfected cells. This system is unaffected by the related antibiotic, bleomycin.
mesh_terms: D000225:Adenine / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D000619:Aminohydrolases / Q000096:biosynthesis; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D001412:Bacillus subtilis; D001435:Bacteriophages / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D002250:Carbon Radioisotopes; D004254:DNA Nucleotidyltransferases / Q000096:biosynthesis; D004267:DNA Viruses; D004279:DNA, Viral / Q000096:biosynthesis / Q000378:metabolism; D010455:Peptides; D010744:Phosphoric Monoester Hydrolases / Q000096:biosynthesis; D010761:Phosphorus Radioisotopes; D011755:Pyrophosphatases / Q000096:biosynthesis; D014316:Tritium; D014764:Viral Proteins / Q000096:biosynthesis; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D000596:Amino Acids; D000903:Antibiotics, Antineoplastic; D002250:Carbon Radioisotopes; D004279:DNA, Viral; D010455:Peptides; D010761:Phosphorus Radioisotopes; D014764:Viral Proteins; D014316:Tritium; D004254:DNA Nucleotidyltransferases; D010744:Phosphoric Monoester Hydrolases; D000619:Aminohydrolases; D011755:Pyrophosphatases; D000225:Adenine