Differences and similarities between guanosine 3',5'-cyclic monophosphate phosphodiesterase and adenosine 3',5'-cyclic monophosphate phosphodiesterase activities in neuroblastoma cells in culture.
There are phosphodiesterase activities in both particulate and supernatant fractions which hydrolyze guanosine 3',5'-cyclic monophosphate (cGMP) and adenosine 3',5'-cyclic monophosphate (cAMP) with an apparent Km of 2-8 muM and with an apparent Km of 44-222 muM. 4-(3-Butoxy-4-methoxybenzyl-2-imidazolidinone (RO20-1724) did not inhibit cGMP phosphodiesterase activity in homogenates of mouse neuroblastoma cells, but markedly inhibited cAMP phosphodiesterase activity. Papaverine and theophylline inhibited both cGMP and cAMP phosphodiesterase activities to about the same extent. The former was more potent than the latter. The specific activity of cGMP phosphodiesterase as a function of protein concentrations first increased and then decreased. The specific activity of cAMP phosphodiesterase decreased under a similar experimental condition.
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000032:analysis* / Q000037:antagonists & inhibitors; D012368:4-(3-Butoxy-4-methoxybenzyl)-2-imidazolidinone / Q000494:pharmacology; D000818:Animals; D002021:Buffers; D002458:Cell Fractionation; D002478:Cells, Cultured; D002999:Clone Cells; D006152:Cyclic GMP; D003851:Deoxyribonucleases; D006801:Humans; D051379:Mice; D009447:Neuroblastoma / Q000201:enzymology*; D010208:Papaverine / Q000494:pharmacology; D010726:Phosphodiesterase Inhibitors; D010727:Phosphoric Diester Hydrolases / Q000032:analysis*; D011506:Proteins / Q000494:pharmacology; D012260:Ribonucleases; D013806:Theophylline / Q000494:pharmacology
D002021:Buffers; D010726:Phosphodiesterase Inhibitors; D011506:Proteins; D012368:4-(3-Butoxy-4-methoxybenzyl)-2-imidazolidinone; D013806:Theophylline; D010208:Papaverine; D003851:Deoxyribonucleases; D012260:Ribonucleases; D010727:Phosphoric Diester Hydrolases; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D006152:Cyclic GMP