[Characteristics of human plasminogen activator and of a fraction of fibrinolytically active plasma enriched with this enzyme].
An activator of plasminogen with specific activity 203 AU/mg was isolated from blood plasma with fibrinolytic activity, obtained from blood of suddenly decreased patients. Specific activity of the plasminogen activator exceeded 88-fold the activity of the initial blood plasma. The protein was identified with a glycoprotein, similar to beta-globulin; its molecular weight was 70,000 as shown by gel filtration; the isoelectric point was at pH 6.2. The plasminogen activator remained stable after heating up to 50 degrees. Effects of pH in an incubation media and of cations on the activity of the plasminogen activator were studied. A fraction containing the fibrinolytic activity and enriched with plasminogen activator was obtained from the blood plasma after fractionation at low temperature with ethanol at definite pH value. The specific fibrinolytic activity in the fraction exceeded 17.6-fold the activity of blood plasma. The fraction exhibited high thrombolytic and antithrombin activities in vitro. It was similar to streptase and streptokinase preparations in its throm-bolytic effect. Relative species specificity was found in studies of the fibrinolytic and antithrombin effects of the fraction containing fibrinolytic activity.
D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects; D003645:Death, Sudden; D004789:Enzyme Activation; D005341:Fibrinolysin / Q000032:analysis; D005342:Fibrinolysis; D006801:Humans; D066298:In Vitro Techniques; D007526:Isoelectric Point; D008970:Molecular Weight; D010960:Plasminogen Activators / Q000032:analysis / Q000302:isolation & purification*; D011817:Rabbits; D013997:Time Factors
D010960:Plasminogen Activators; D005341:Fibrinolysin
Kharakteristika aktivatora plazminogena cheloveka i fraktsii fibrinoliticheski aktivnoĭ plazmy, obogashchennoĭ étim fermentom.