title: Catabolite repression of Pseudomonas aeruginosa amidase: isolation of promotor mutants.
abstract: Among mutants of Pseudomonas aeruginosa isolated from fluoroacetamide medium were some which synthesized amidase at about 5% of the rate of the parent constitutive strain, PAC101. Seven fluoroacetamide-resistant mutants with low amidase activity gave rise to secondary mutant strains on succinate+butyramide plates. One appeared to be an 'up-promotor' mutant and synthesized amidase at a high rate. This mutant, PAC433, was not stimulated by cyclic-AMP and was much less sensitive to catabolite repression by succinate. The mutation conferring resistance to catabolite repression was cotransduced at a frequency of 96% (26/27) with the amidase genes amiR, amiE. Five other revertants had catabolite repression-resistance mutations which were linked to the amidase genes and these also were probably promotor mutants. One strain had a mutation conferring resistance to catabolite repression which was unlinked to the amidase genes.
mesh_terms: D000081:Acetamides / Q000378:metabolism; D000085:Acetates / Q000378:metabolism; D000577:Amides / Q000378:metabolism; D000581:Amidohydrolases / Q000096:biosynthesis*; D000242:Cyclic AMP / Q000494:pharmacology; D004794:Enzyme Repression; D005461:Fluorine; D008769:Methylnitronitrosoguanidine; D009153:Mutagens; D009154:Mutation; D009876:Operon; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000378:metabolism; D011773:Pyruvates / Q000378:metabolism; D013386:Succinates / Q000378:metabolism; D014161:Transduction, Genetic
chemical_list: D000081:Acetamides; D000085:Acetates; D000577:Amides; D009153:Mutagens; D011773:Pyruvates; D013386:Succinates; D008769:Methylnitronitrosoguanidine; D005461:Fluorine; D000242:Cyclic AMP; D000581:Amidohydrolases