[Differences in the buoyant density of mitochondria from hepatoma, Krebs ascitic tumor II and normal liver].
Mitochondria isolated from normal liver, experimental hepatoma, and Krebs II ascites tumor tissues in mice were examined by isopycnic centrifugation in a sucrose density gradient (25--50%). Normal liver mitochondria were shown to be characterized by two visible zones, while those of tumors were distributed homogeneously. Such a distribution pattern was also confirmed by the investigation of marker enzyme activities (glucose-6-phosphatase and cytochromoxidase). A comparison of homologous tissues (hepatoma--liver) has evidenced that hepatoma mitochondria are "lighter" even than the "light" fraction of normal ones, that seems to evidence the disturbances in the biogenesis of these organelles. The results obtained are in a good agreement with the data reported by S. A. Neifakh on alterations in the structure of tumor mitochondrial membranes.
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D002287:Carcinoma, Krebs 2 / Q000473:pathology*; D002500:Centrifugation, Isopycnic; D008113:Liver Neoplasms / Q000473:pathology*; D051379:Mice; D008815:Mice, Inbred Strains; D008930:Mitochondria, Liver
Razlichiia v plavucheĭ plotnosti mitokhondriĭ gepatomy astsitnoĭ opukholi Krebs II i normal'noĭ pecheni