Synthesis of complementary RNA containing polyadenylic acid by Sendai virions in vitro.
Sendai virus synthesized, in vitro, [32P]AMP- and [3H]AMP-labeled RNA that ranged in size from 3 to 25S with major peaks at 7S and 13S. Both labeled products were predominantly single-stranded RNA and were complementary in base sequence to 50S virion RNA. Passage of the 3 to 25S in vitro RNA transcripts through a polyuridylic acid-cellulose column revealed that only the larger (predominantly 18S) RNA transcripts contained polyadenylic acid[poly(A)] segments capable of binding to the column. After treatment with a combination of RNase A and T1, the majority of the in vitro poly(A) sedimented at 6S although the product ranged in size from 3 to 9S. Proof that the RNase-resistant material was indeed poly(A) was obtained by nearest-neighbor analysis when 95% of the radioactivity was recovered in AMP.
D001483:Base Sequence; D002474:Cell-Free System; D046508:Culture Techniques; D010222:Parainfluenza Virus 1, Human / Q000032:analysis / Q000201:enzymology / Q000378:metabolism*; D011061:Poly A / Q000032:analysis / Q000096:biosynthesis*; D012335:RNA, Ribosomal / Q000378:metabolism; D012367:RNA, Viral / Q000032:analysis / Q000096:biosynthesis*; D012260:Ribonucleases / Q000378:metabolism
D012335:RNA, Ribosomal; D012367:RNA, Viral; D011061:Poly A; D012260:Ribonucleases