Characterization of the T antigen induced by SV40.
3 or 4 antigenic SV40 specific components were established in hamster tumors induced by SV40-transformed cells, using linear sucrose gradient centrifugation and Sephadex G-200 column chromatography. The lightest component, approximate molecular weight 21,000, did not inhibit the nuclear staining of SV40-transformed cells in the fluorescent antibody inhibition test.
D000429:Alcohol Oxidoreductases; D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis*; D002471:Cell Transformation, Neoplastic; D002499:Centrifugation, Density Gradient / Q000379:methods; D006224:Cricetinae; D013539:Simian virus 40 / Q000276:immunology*; D013395:Sucrose; D014462:Ultrafiltration
D000951:Antigens, Neoplasm; D013395:Sucrose; D000429:Alcohol Oxidoreductases