Phosphonopyruvic acid: A probable precursor of phosphonic acids in cell-free preparation of Tetrahymena.
1. In cell-free preparations of Tetrahymena, doubly labelled [32P]phosphoenol-[3-14C]pyruvate gives rise to 2-aminoethylphosphonate and 2-amino-3-phosphonopropionate, labelled with the two isotopes in the same ratio as the starting compound. The result is consistent with an intra-molecular rearrangement of phosphoenolpyruvate in the biosynthetic sequence of carbon-phosphorus bond formation. 2. Incubation of [32P]phosphoenolpyruvate with the same preparation, followed by treatment with 2,4-dinitrophenylhydrazine, yielded labelled hydrazones. When these were subjected to hydrogenolysis, the radioactivity was recovered in 2-aminoethylphosphonate and 2-amino-3-phosphonopropionate, suggesting that 2-phosphonoacetaldehyde and 3-phosphonopyruvic acid were probable precursors of the aminoalkylphosphonic acids. 3. Radioactivity from 2-amino-3-phosphono-[3-14C]propionic acid was incorporated into 2-aminoethylphosphonic acid, but incorporation of the radioactivity into lipids was negligible.
D000818:Animals; D002474:Cell-Free System; D004926:Escherichia coli / Q000201:enzymology; D063065:Organophosphonates / Q000378:metabolism*; D011772:Pyruvate, Orthophosphate Dikinase / Q000378:metabolism; D011773:Pyruvates / Q000378:metabolism*; D013768:Tetrahymena / Q000378:metabolism*
D063065:Organophosphonates; D011773:Pyruvates; D011772:Pyruvate, Orthophosphate Dikinase