The effect of two isomeric octadecenoic acids on the lipid metabolism and growth of Novikoff hepatoma cells.
The origin and metabolism of octadecenoic acid (18 : 1) was examined in intact Novikoff rat hepatoma cells by using labeled precursors and two isomeric octadecenoic acids which differed in their abilities to stimulate cell growth in a serum-free medium. The isomers (ci-6-18 : 1 and cis-9-18 : 1) were measured in the cellular lipid by ozonolysis and reduction of the ozonides. The results indicate that the 18 : 1 fatty acid accumulated in the cell lipid by uptake of the preformed acid from the medium. The cis-9-18 : 1 to 16 : 1 and 20 : 1 fatty acids by chain shortening and chain elongation. Both isomers inhibited de novo fatty acid synthesis from acetate by cells suspended in a serum-free medium. The isomers did not exert coordinate control of both fatty acid and cholesterol biosynthesis in the Novikoff cells.
D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D003470:Culture Media; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D007536:Isomerism; D007700:Kinetics; D050356:Lipid Metabolism; D008113:Liver Neoplasms / Q000378:metabolism*; D013329:Structure-Activity Relationship
D003470:Culture Media; D005231:Fatty Acids, Unsaturated