Radioisotopic labeling of human papovavirus (BK) by iodination and reductive alkylation.
Purified virions of the GS strain of the BK group of human papovaviruses were labeled with 125I using chloramine T or lactoperoxidase or with tritium using sodium borohydride. All viral polypeptides were labeled. Tryptic digests of iodinated VP1 were analyzed.
D001739:BK Virus / Q000648:ultrastructure*; D001894:Borohydrides; D002700:Chloramines; D005069:Evaluation Studies as Topic; D006801:Humans; D007457:Iodine Radioisotopes; D007553:Isotope Labeling / Q000379:methods*; D007784:Lactoperoxidase; D010455:Peptides / Q000032:analysis; D011120:Polyomavirus / Q000648:ultrastructure*; D014556:Urine / Q000382:microbiology; D014764:Viral Proteins / Q000032:analysis
D001894:Borohydrides; D002700:Chloramines; D007457:Iodine Radioisotopes; D010455:Peptides; D014764:Viral Proteins; D007784:Lactoperoxidase