Extracellular cyclic-amp phosphodiesterase regulation in agar plate cultures of Dictyostelium discoideum.
Extracellular cyclic-AMP phosphodiesterase and the inhibitor of this enzyme is tested in agar plate cultures of two Dictyostelium discoideum wild-type strains and in a mutant which lacks the inhibitor. Under the conditions used, the phosphodiesterase inhibitor is formed in both wild-type strains either before or in an early stage of cell aggregation. During aggregation of one strain the phosphodiesterase activity is extremely low, excluding a necessary function of the enzyme in the aggregation process.
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002449:Cell Aggregation; D004023:Dictyostelium / Q000166:cytology / Q000201:enzymology*; D009235:Myxomycetes / Q000201:enzymology*; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*
D010727:Phosphoric Diester Hydrolases; D015105:3',5'-Cyclic-AMP Phosphodiesterases