Oligo(A) not coded by DNA generating 3'-terminal heterogeneity in a lambda phage RNA.
An RNA species from Escherichia coli infected with phage lambda was purified by hybridization to lambda 1-strand DNA and shown to have variable length. This variability was due to a variable number of adenylate residues attached to the 3' end of the molecule. Pancreatic RNase treatment of the RNA-DNA hybrid removed the 3'-terminal adenylate residues, generating a homogeneous RNA molecule terminating with -Up. The results indicate the presence of adenylate residues not coded by the DNA template at the terminus of this transcript from intact cells.
D000227:Adenine Nucleotides; D001483:Base Sequence; D003090:Coliphages / Q000378:metabolism*; D004588:Electrophoresis, Cellulose Acetate; D004590:Electrophoresis, Paper; D004926:Escherichia coli / Q000378:metabolism; D005815:Genetic Code; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000032:analysis / Q000378:metabolism*; D010179:Pancreas / Q000201:enzymology; D010727:Phosphoric Diester Hydrolases; D012367:RNA, Viral / Q000378:metabolism*; D012260:Ribonucleases; D012910:Snake Venoms
D000227:Adenine Nucleotides; D009841:Oligonucleotides; D012367:RNA, Viral; D012910:Snake Venoms; D012260:Ribonucleases; D010727:Phosphoric Diester Hydrolases