Effects of arachidonic acid on RNA metabolism in mammary gland explants of mice.
Arachidonic acid stimulated 3H-uridine incorporation into RNA in mammary gland explants of mice in a manner similar to that of prolactin. The onset of the effects of both prolactin and arachidonic acid occurred following a 2-4 hour lag period. Further, effects of maximally stimulatory concentrations of these agents were nonadditive. Finally, indomethacin abolished the effects of both prolactin and arachidonic acid. A tenable hypothesis to explain these data is that the action of prolactin in the mammary gland may be mediated by an increased availability of arachidonic acid followed by a subsequent enhanced rate of formation of the prostaglandins.
D000818:Animals; D001095:Arachidonic Acids / Q000494:pharmacology*; D001940:Breast / Q000378:metabolism*; D003994:Bucladesine / Q000494:pharmacology; D006152:Cyclic GMP / Q000494:pharmacology; D005260:Female; D007213:Indomethacin / Q000494:pharmacology; D008041:Linoleic Acids / Q000494:pharmacology; D051379:Mice; D009829:Oleic Acids / Q000494:pharmacology; D011247:Pregnancy; D011388:Prolactin / Q000494:pharmacology; D011458:Prostaglandins E / Q000494:pharmacology; D011460:Prostaglandins F / Q000494:pharmacology; D011803:Quinine / Q000494:pharmacology; D012313:RNA / Q000096:biosynthesis / Q000378:metabolism*; D013229:Stearic Acids / Q000494:pharmacology; D013806:Theophylline / Q000494:pharmacology; D014529:Uridine / Q000378:metabolism
D001095:Arachidonic Acids; D008041:Linoleic Acids; D009829:Oleic Acids; D011458:Prostaglandins E; D011460:Prostaglandins F; D013229:Stearic Acids; D012313:RNA; D003994:Bucladesine; D011388:Prolactin; D011803:Quinine; D013806:Theophylline; D006152:Cyclic GMP; D014529:Uridine; D007213:Indomethacin