DNA-binding proteins from Novikoff hepatoma cells.
In 0.05 M NaCl, 6-8% of the total soluble proteins from Novikoff hepatoma cells bind rapidly and reversibly to columns containing either heterologous or homologous DNA adsorbed to cellulose. These proteins can be eluted by buffer containing 2.0 M NaCl. 0.5-1% of the total protein exhibits a 7-17-fold preference for rat DNA over Escherichia coli DNA. 1-1.5% of the proteins bind DNA so strongly that elution cannot be effected by 4.0 M NaCl but can be accomplished by deoxyribonuclease I treatment of the columns. DNA-binding proteins eluted by 2.0 M NaCl were labeled with 125I or 131I and characterized by sodium dodecylsulfate-polyacrylamide gel electrophoresis and isoelectric focusing. These experiments indicate that DNA-binding proteins represent a discrete subset of the total soluble protein. Many similarities were noted between the major components of the homologous and heterologous DNA-binding fractions.
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000032:analysis*; D002482:Cellulose; D002846:Chromatography, Affinity; D004247:DNA; D004269:DNA, Bacterial; D004926:Escherichia coli; D007700:Kinetics; D008113:Liver Neoplasms; D008722:Methods; D009363:Neoplasm Proteins / Q000302:isolation & purification*; D009374:Neoplasms, Experimental / Q000032:analysis; D011485:Protein Binding; D051381:Rats; D013045:Species Specificity
D004269:DNA, Bacterial; D009363:Neoplasm Proteins; D002482:Cellulose; D004247:DNA