Detection of memory cells by chemical modification of the lymphocyte cell surface.
Stringent alloantigen requirements, necessary for the differentiation of human memory cells into specific secondary cytolytic T cells (2 degrees CTL), can be bypassed by chemical modification of memory cells with the mitogenic oxidising agent, galactose oxidase. Treatment of memory cells generated in a long-term primary mixed lymphocyte culture with neuraminidase and galactose oxidase (NAGO) results in the differentiation of memory cells into 2 degrees CTL. In contrast, treatment of unprimed cells with NAGO does not result in CTL production despite the proliferation resulting from such treatment.
D002462:Cell Membrane / Q000276:immunology; D055598:Chemical Phenomena; D002621:Chemistry; D003602:Cytotoxicity, Immunologic; D005692:Galactose Oxidase / Q000494:pharmacology; D006801:Humans; D007156:Immunologic Memory; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000276:immunology*; D009439:Neuraminidase / Q000494:pharmacology
D005692:Galactose Oxidase; D009439:Neuraminidase