Identification of collagen-anticollagen immune complexes by collagenase-digestion.
A method for the detection of collagen-anti-collagen immune complexes using collagenase of bacterial origin is described. Anti-collagen-titers are determined in passive haemagglutination before and after collagenase digestion of samples containing preformed immune complexes. An increase in anti-collagen titers following the enzymatic treatment suggests the presence of collagen-anti-collagen immune complexes. Both solbule and carrier-bound collagenase proved to be equally effective in the digestion of complex-bound collagen, if in vitro prepared collagen-anti-collagen complexes were investigated. Original titers of partially or totally inhibited antisera to collagen could be completely restored by this method.
D000936:Antigen-Antibody Complex / Q000032:analysis*; D003094:Collagen / Q000276:immunology*; D003545:Cysteine / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D006801:Humans; D008722:Methods; D003012:Microbial Collagenase / Q000037:antagonists & inhibitors / Q000378:metabolism; D012995:Solubility
D000936:Antigen-Antibody Complex; D003094:Collagen; D004492:Edetic Acid; D003012:Microbial Collagenase; D003545:Cysteine