The 5' terminal structure of the methylated mRNA synthesized in vitro by vesicular stomatitis virus.
The 5' terminal structure of the mRNA synthesized in vitro by the virion-associated RNA polymerase of vesicular stomatitis virus in the presence of S-adenosyl-L-methione consists of 7-methyl guanosine linked to 2'-O-methyl adenosine through a 5'-5' pyrophosphate bond as m7G(5')ppp(5')A-m-p ... The alpha and beta phosphated of GTP and alpha phosphate of ATP are incorporated into the blocked 5' terminal structure.
D000241:Adenosine / Q000032:analysis; D000255:Adenosine Triphosphate / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D002474:Cell-Free System; D002848:Chromatography, DEAE-Cellulose; D002854:Chromatography, Paper; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D011756:Diphosphates / Q000032:analysis; D004590:Electrophoresis, Paper; D006151:Guanosine / Q000032:analysis; D006160:Guanosine Triphosphate / Q000378:metabolism; D008745:Methylation; D009841:Oligonucleotides / Q000032:analysis; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D011755:Pyrophosphatases / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis* / Q000096:biosynthesis; D012367:RNA, Viral / Q000032:analysis* / Q000096:biosynthesis; D012260:Ribonucleases / Q000378:metabolism; D012436:S-Adenosylmethionine / Q000378:metabolism; D014721:Vesicular stomatitis Indiana virus / Q000032:analysis* / Q000201:enzymology
D011756:Diphosphates; D009841:Oligonucleotides; D012333:RNA, Messenger; D012367:RNA, Viral; D006151:Guanosine; D012436:S-Adenosylmethionine; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D012321:DNA-Directed RNA Polymerases; D012260:Ribonucleases; D000469:Alkaline Phosphatase; D010727:Phosphoric Diester Hydrolases; D011755:Pyrophosphatases; D000241:Adenosine