LH- and FSH-stimulating of adenylate cyclase in seminiferous tubules from young rats: functional FSH and LH receptors unmasked by homogenization.
LH- and FSH-sensitive adenylate cyclase activity was present in homogenates of whole testis tissue as well as in microdissected seminiferous tubules derived from young rats. In homogenates of seminiferous tubules a single adenylate cyclase appears to interact with both LH and FSH through separate hormone-specific receptors. Disruption of testis tissue by homogenization exposes functional FSH and LH receptors which are inaccessible to the hormones in intact cell preparations. These results indicate that in certain seminiferous tubule cell types only a fraction of the total functional receptors present is accessible to the cell surface for interaction with hormone.
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D005640:Follicle Stimulating Hormone / Q000378:metabolism / Q000494:pharmacology*; D007986:Luteinizing Hormone / Q000378:metabolism / Q000494:pharmacology*; D008297:Male; D003012:Microbial Collagenase; D008854:Microscopy, Electron; D011485:Protein Binding; D051381:Rats; D011956:Receptors, Cell Surface; D012671:Seminiferous Tubules / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D013737:Testis / Q000378:metabolism*
D011956:Receptors, Cell Surface; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D003012:Microbial Collagenase; D000262:Adenylyl Cyclases