Evidence that detergent-reactivated interferons are not renatured.
The sedimentation rate of human leukocyte interferon (HLIF) reactivated from sodium dodecyl sulphate (SDS) solution was studied by glycerol gradient centrifugation and compared to that of native HLIF. Reactivated HLIF consistently sedimented faster than native HLIF, indicating that full recovery of antiviral activity does not require renaturation of the entire interferon molecule.
D000818:Animals; D000998:Antiviral Agents; D001769:Blood; D002417:Cattle; D002499:Centrifugation, Density Gradient; D003470:Culture Media; D006801:Humans; D007372:Interferons / Q000032:analysis* / Q000096:biosynthesis / Q000494:pharmacology; D007962:Leukocytes / Q000378:metabolism; D008970:Molecular Weight; D011489:Protein Denaturation; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology*; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects
D000998:Antiviral Agents; D003470:Culture Media; D012967:Sodium Dodecyl Sulfate; D007372:Interferons