title: 6-Phosphogluconate dehydratase deficiency in pleiotropic carbohydrate-negative mutant strains of Pseudomonas aeruginosa.
abstract: Mutants of Pseudomonas aeruginosa, strain PAO, have been isolated that are unable to grow on mannitol, glucose, gluconate, or 2-ketogluconate, cut that exhibit wild-type growth on pyruvate, lactate, citrate, succinate, or acetate. Although some of these mutants were also unable to grow on glycerol, the mutations formed a single linkage group by quantitative transductional analysis with phage F116 on glucose minimal agar medium. Cell extracts of all mutant strains were either lacking or severely deficient in 6-phosphogluconate dehydratase activity. Glu+ transductants derived from mutant strains that retained the wild-type ability for growth at the expense of glycerol also regained the ability to grow on all C-6 compounds. Although a role for the pentose phosphate pathway in the catabolism of C6 substrates was not found, a functional Entner-Doudoroff pathway appears to be essential for the catabolism of mannitol, glucose, gluconate, and 2-ketogluconate.
mesh_terms: D000446:Aldehyde-Lyases / Q000378:metabolism; D002264:Carboxylic Acids / Q000378:metabolism*; D002474:Cell-Free System; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism; D005942:Gluconates; D005947:Glucose / Q000378:metabolism; D005956:Glucose-6-Phosphate Isomerase / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D006836:Hydro-Lyases / Q000096:biosynthesis* / Q000378:metabolism; D008353:Mannitol / Q000378:metabolism; D009154:Mutation; D009943:Organophosphorus Compounds; D010641:Phenotype; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D010770:Phosphotransferases / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000378:metabolism; D014161:Transduction, Genetic
chemical_list: D002264:Carboxylic Acids; D005942:Gluconates; D009943:Organophosphorus Compounds; D008353:Mannitol; D005954:Glucosephosphate Dehydrogenase; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D010770:Phosphotransferases; D000446:Aldehyde-Lyases; D005634:Fructose-Bisphosphate Aldolase; D006836:Hydro-Lyases; D010751:Phosphopyruvate Hydratase; D005956:Glucose-6-Phosphate Isomerase; D005947:Glucose