In vitro cultivation of cells from the chorioallantoic membrane of chick embryos.
By treatment of chorioallantoic membranes from embryonated eggs with collagenase and hyaluronidase before the conventional application of trypsin cells could be grown in culture which supported growth of a large variety of myxoviruses, herpesviruses, avian reoviruses and the infectious bronchitis virus of chickens. The cultures could be used for sensitive plaque assays and neutralization tests.
D000482:Allantois / Q000166:cytology*; D000818:Animals; D002469:Cell Separation; D002478:Cells, Cultured / Q000382:microbiology*; D002642:Chick Embryo / Q000166:cytology*; D002645:Chickens / Q000276:immunology; D002823:Chorion / Q000166:cytology; D003332:Coronaviridae / Q000254:growth & development; D003588:Cytopathogenic Effect, Viral; D005321:Extraembryonic Membranes / Q000166:cytology*; D005347:Fibroblasts / Q000382:microbiology; D006564:Herpesviridae / Q000254:growth & development; D006736:Horses; D006801:Humans; D006821:Hyaluronoglucosaminidase / Q000494:pharmacology; D007106:Immune Sera; D003012:Microbial Collagenase / Q000494:pharmacology; D009500:Neutralization Tests; D009975:Orthomyxoviridae / Q000254:growth & development; D010252:Paramyxoviridae / Q000254:growth & development; D011817:Rabbits / Q000276:immunology; D012087:Reoviridae / Q000254:growth & development; D014357:Trypsin / Q000494:pharmacology; D010948:Viral Plaque Assay; D014776:Virus Cultivation; D014779:Virus Replication
D007106:Immune Sera; D006821:Hyaluronoglucosaminidase; D014357:Trypsin; D003012:Microbial Collagenase