Gastric mucosal blood flow and pepsin secretion in dogs--stimulation by 13-nle-motilin.
In response to graded doses of intravenous 13-norleucine-motilin (13-nle-motilin)--a synthetic analogue of motilin and biologically equivalent to the natural polypeptide-, gastric mucosal blood flow (GMBF) in canine vagally denervated fundic pouches was studied using the aminopyrine clearance technique. As 13-nle-motilin did not exert any detectable effect on gastric secretion of hydrogen ions, intraluminal instillation of 160 mM HCl was used to provide a pH gradient allowing aminopyrine to move into the pouch lumen. With increasing doses of 13-nle-motilin, GMBF increased to 148% of control values; pepsin secretion  - due to augmented pepsin concentration - rose concomitantly. Enhanced pepsin secretion was not accompanied by an increase in cyclic 3',5'-adenosine monophosphate secretion.
D000632:Aminopyrine / Q000378:metabolism; D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D005260:Female; D005753:Gastric Mucosa / Q000098:blood supply* / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009037:Motilin / Q000031:analogs & derivatives; D009646:Norleucine / Q000031:analogs & derivatives / Q000494:pharmacology; D010434:Pepsin A / Q000378:metabolism*; D010455:Peptides / Q000494:pharmacology*; D012039:Regional Blood Flow / Q000187:drug effects; D012636:Secretory Rate / Q000187:drug effects; D014630:Vagus Nerve / Q000502:physiology
D010455:Peptides; D000632:Aminopyrine; D009037:Motilin; C008680:motilin, Nle(13)-; D009646:Norleucine; D000242:Cyclic AMP; D010434:Pepsin A