Rescue of a rat tropic rat hepatoma virus pseudotype Kirsten sarcoma virus by co-cultivation of hepatoma tissue culture cells with K-NRK cells.
A rat tropic rat hepatoma virus pseudotype Kirsten sarcoma virus has been rescued from a co-culture of rat hepatoma tissue culture cells, HTC-HI, with Kirsten murine sarcoma transformed non-producer rat kidney cells (K-NRK). The virus complex, RHHV-KiMSV, released from the co-culture exhibited cell transformation ability on normal rat kidney (NRK) cells and showed a restricted host range limited to rat embryo fibroblasts and other normal rat cell lines. Evidence derived from indirect immunofluorescence assays demonstrated the association of rat helper virus specific gs-I and gs-3 antigens with RHHV-KiMSV transformed cells. RHHVKiMSV is currently being cultured at a titre as high as 10(4) f.f.u./ml in tissue culture, and thus offering opportunity for biochemical studies of rat viruses.
D000818:Animals; D001973:Bromodeoxyuridine / Q000494:pharmacology; D006528:Carcinoma, Hepatocellular; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D017988:Gammaretrovirus / Q000254:growth & development* / Q000276:immunology; D006378:Helper Viruses / Q000254:growth & development / Q000276:immunology; D007065:Idoxuridine / Q000494:pharmacology; D007668:Kidney; D008113:Liver Neoplasms; D051381:Rats; D012190:Retroviridae / Q000254:growth & development* / Q000276:immunology; D014779:Virus Replication
D001973:Bromodeoxyuridine; D007065:Idoxuridine