title: The isolation of collagen-associated proteoglycan from bovine nasal cartilage and its preferential interaction with alpha2 chains of type I collagen.
abstract: A collagen complex from bovine nasal cartilage was prepared by extraction of the tissue with 3M-MgCl2 solutions, by using two different procedures. When it was compared with calf skin acid-soluble tropocollagen by polyacrylamide-gel electrophoresis, the 3M-MgCl2-soluble cartilage collagen in the complex appeared to be predominantly type I in nature, consisting of both alpha1 and alpha2 chains. The soluble cartilage collagens were digested with purified bacterial collagenase, and the soluble digests were fractionated on Sepharose 4B. Hydroxyproline-free proteoglycan was isolated in the excluded volume of the column eluate, and this was found to be an aggregate which could be dissociated to link proteins and proteoglycan subunit by equilibrium-density-gradient centrifugation in a CsCl-4M-guanidinium chloride gradient. Interaction with calf skin-soluble tropocollagen was studied by CM-cellulose chromatography. The link-protein system did not interact, but proteoglycan from the bottom of the gradient did interact. In addition, when proteoglycan subunit was allowed to interact with collagen, there was a preferential binding to the alpha2 and beta12 components, and this effect was also observed with the proteoglycan material obtained from the collagenase digests of 3M-MgCl2-soluble cartilage collagen complexes. However, specificity for alpha2 and beta12 chains was not exhibited by chondroitin sulphate glycosaminoglycan, and it is therefore concluded that preference for alpha2 and beta12 chains is a function of the intact proteoglycan structure.
mesh_terms: D000818:Animals; D001665:Binding Sites; D002356:Cartilage / Q000032:analysis*; D002417:Cattle; D003094:Collagen / Q000302:isolation & purification*; D006025:Glycosaminoglycans / Q000302:isolation & purification*; D046911:Macromolecular Substances; D008274:Magnesium; D003012:Microbial Collagenase; D009300:Nasal Septum / Q000032:analysis*; D010446:Peptide Fragments / Q000032:analysis; D011485:Protein Binding; D011509:Proteoglycans / Q000302:isolation & purification*; D012995:Solubility; D014574:Uronic Acids / Q000032:analysis
chemical_list: D006025:Glycosaminoglycans; D046911:Macromolecular Substances; D010446:Peptide Fragments; D011509:Proteoglycans; D014574:Uronic Acids; D003094:Collagen; D003012:Microbial Collagenase; D008274:Magnesium