A simple technique for testing the in vitro response of rabbit lymphocytes to PHA and allogeneic cells.
Lymphocytes of rabbits can be separated from small quantities of heparinized whole blood using a simple density gradient of Ficoll-Ronpacon 1.09. This separation technique yields a pure suspension of viable cells allowing reproducible results from cultures stimulated either with PHA or allogeneic lymphocytes isolated by the same technique.
D000818:Animals; D002469:Cell Separation / Q000379:methods; D002499:Centrifugation, Density Gradient / Q000379:methods; D004305:Dose-Response Relationship, Drug; D005362:Ficoll; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed / Q000379:methods*; D010835:Phytohemagglutinins / Q000494:pharmacology; D011817:Rabbits / Q000276:immunology*
D010835:Phytohemagglutinins; D005362:Ficoll