Tissue-specific chromosomal non-histone protein interactions with DNA.
A group of chromosomal non-histone proteins with affinity for DNA (NP) was isolated from rat liver and Novikoff hepatoma. This fraction, which represents less than 5% of the total chromatin protein content, binds preferentially to unique, double-stranded sequences of fractionated homologous DNA. The interactions are strong at low ionic strength (Km = 6.7 X 10(-9) M) and decrease with rising salt concentration. Complexes of the NP protein fraction with homologous DNA are immunologically tissue-specific. As determined by microcomplement fixation, the NP proteins in Novikoff hepatoma are associated with the transcriptionally active, diffuse fraction of chromatin.
D000818:Animals; D001665:Binding Sites; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D002875:Chromosomes / Q000378:metabolism*; D003168:Complement Fixation Tests; D004273:DNA, Neoplasm / Q000378:metabolism; D003852:Deoxyribonucleoproteins / Q000276:immunology; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008113:Liver Neoplasms / Q000378:metabolism*; D046911:Macromolecular Substances; D008970:Molecular Weight; D009374:Neoplasms, Experimental / Q000378:metabolism; D009994:Osmolar Concentration; D011485:Protein Binding; D051381:Rats; D013154:Spleen / Q000378:metabolism
D004273:DNA, Neoplasm; D003852:Deoxyribonucleoproteins; D046911:Macromolecular Substances