[Estimation of free cortisol in urine (author's transl)].
Radio-immunological estimation of free cortisol in urine in a group of normal persons showed a median daily excretion of 42,5 +/- 22,8 mug (n = 31) using radio-loss-labelling, extraction, and thin layer chromatography. After simple extraction the median value was 54.1 +/- 15.3 mug/d (n = 10). Radioimmunological estimation in simple urine extract seems to be sufficient for routine clinical purposes. The estimation of free cortisol in urine permits a better differentiation between normal adrenal function and adrenal hyperactivity than the determination of 17-hydroxycorticosteroids (17-OHCS). This is shown by comparison of 1) the frequency distribution of the basal values of 17-OHCS and free cortisol in normal persons and in Cushing's disease, 2) the suppression effect of dexamethasone on 17-OHCS and free cortisol in Cushing patients, and 3) the stimulatory effect of ACTH on 17-OHCS and free cortisol in normal persons.
D015065:17-Hydroxycorticosteroids / Q000652:urine; D000308:Adrenocortical Hyperfunction / Q000652:urine; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D002855:Chromatography, Thin Layer; D003480:Cushing Syndrome / Q000652:urine; D003907:Dexamethasone / Q000494:pharmacology; D006801:Humans; D006854:Hydrocortisone / Q000652:urine*; D011863:Radioimmunoassay / Q000379:methods
D015065:17-Hydroxycorticosteroids; D003907:Dexamethasone; D000324:Adrenocorticotropic Hormone; D006854:Hydrocortisone
Die Bestimmung von freiem Cortisol im Urin