DNA-cellulose column chromatography of phosphorylated nucleolar nonhistone proteins.
A salt-extraction procedure was used to isolate a nucleolar nonhistone protein fraction, containing [32P]phosphoserine, from the nucleoli of Novikoff hepatoma ascites cells. These proteins are similar in amino-acid composition to whole nuclear (chromosomal) nonhistone proteins. DNA-cellulose column chromatography showed that this fraction contains DNA-binding phosphoproteins, some of which will bind only to homologous (Novikoff) nucleolar or nuclear DNA.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001665:Binding Sites; D006528:Carcinoma, Hepatocellular / Q000032:analysis; D002846:Chromatography, Affinity; D004247:DNA; D008113:Liver Neoplasms / Q000032:analysis; D009374:Neoplasms, Experimental / Q000032:analysis; D009698:Nucleoproteins / Q000302:isolation & purification*; D010750:Phosphoproteins / Q000302:isolation & purification*; D011485:Protein Binding; D051381:Rats
D000596:Amino Acids; D009698:Nucleoproteins; D010750:Phosphoproteins; D004247:DNA