title: Minimal growth requirements for Clostridium perfringens and isolation of auxotrophic mutants.
abstract: The minimal growth requirements for two strains of Clostridium perfringens were defined, and both synthetic and semisynthetic plating media were developed. Plate counts of the wild-type strains on both of these minimal media were equivalent to those on complex media. A number of auxotrophic mutants of each strain were isolated, and their phenotypes were defined.
mesh_terms: D000225:Adenine / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D000693:Anaerobiosis; D003016:Clostridium perfringens / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D003470:Culture Media; D007785:Lactose / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D009153:Mutagens; D009154:Mutation; D009539:Nicotinic Acids / Q000378:metabolism; D009604:Nitrosoguanidines; D010641:Phenotype; D011392:Proline / Q000378:metabolism; D012492:Salts / Q000378:metabolism; D013831:Thiamine / Q000378:metabolism; D013864:Thioglycolates; D014498:Uracil / Q000378:metabolism; D014815:Vitamins / Q000378:metabolism
chemical_list: D000596:Amino Acids; D003470:Culture Media; D009153:Mutagens; D009539:Nicotinic Acids; D009604:Nitrosoguanidines; D012492:Salts; D013864:Thioglycolates; D014815:Vitamins; D014498:Uracil; D011392:Proline; D007785:Lactose; D000225:Adenine; D008239:Lysine; D013831:Thiamine