Replication of colicinogenic factor E1 DNA in plasmolysed Escherichia coli cells. Coupling of DNA replication and RNA synthesis.
Plasmolysed chloramphenicol-treated Escherichia coli cells carrying the colicinogenic factor E1 utilize deoxynucleoside triphosphates for the semi-conservative synthesis of Col E1 DNA. Col E1 DNA replication in plasmolysed cells can be dissociated into two temporally separated processes: (a) a rifampicin-sensitive RNA synthesis, which is stimulated by adenosine 3':5'-monophosphate (cyclic AMP) and requires all four ribonucleoside triphosphates and (b) an ATP-dependent DNA synthesis, which is inhibited by arabinosylnucleoside triphosphates and sulfhydryl-blocking reagents. Thes two processes exhibit different sensitivities to inhibition by polyamines and actinomycin D.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D001976:Bromouracil / Q000494:pharmacology; D002701:Chloramphenicol / Q000494:pharmacology; D003087:Colicins; D000242:Cyclic AMP / Q000494:pharmacology; D003597:Cytosine Nucleotides / Q000378:metabolism; D004261:DNA Replication / Q000187:drug effects*; D003609:Dactinomycin / Q000494:pharmacology; D003854:Deoxyribonucleotides / Q000378:metabolism; D004492:Edetic Acid / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*; D009994:Osmolar Concentration; D011073:Polyamines / Q000494:pharmacology; D012329:RNA, Bacterial / Q000096:biosynthesis*; D012265:Ribonucleotides / Q000378:metabolism; D012293:Rifampin / Q000494:pharmacology; D013439:Sulfhydryl Reagents / Q000494:pharmacology; D013696:Temperature; D013942:Thymine Nucleotides / Q000378:metabolism
D003087:Colicins; D003597:Cytosine Nucleotides; D003854:Deoxyribonucleotides; D011073:Polyamines; D012329:RNA, Bacterial; D012265:Ribonucleotides; D013439:Sulfhydryl Reagents; D013942:Thymine Nucleotides; D003609:Dactinomycin; D001976:Bromouracil; D002701:Chloramphenicol; D000255:Adenosine Triphosphate; D004492:Edetic Acid; D000242:Cyclic AMP; D012293:Rifampin