Regulation of Membrane Flexibility in Human Erythrocytes.
We have used spin-labels to detect prostaglandin E induced changes in erythrocyte membranes. The observed changes in spin-label resonance spectra can be mimicked in erythrocyte ghosts by loading them with cAMP or cGMP. These changes can also be observed by adding either of these cyclic nucleotides to intact cells. This entry of cyclic nucleotides into intact cells is blocked by an inhibitor of the anion channel. We suggest that the observed changes in paramagnetic resonance spectra are due to changes in lipid "fluidity" that are brought about by changes in the biochemical state of membrane-associated proteins (such as spectrin) and in the direct or indirect biophysical interactions of these proteins with membrane lipids.
D001557:Benzenesulfonates / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology; D002462:Cell Membrane / Q000032:analysis / Q000187:drug effects; D000242:Cyclic AMP / Q000008:administration & dosage / Q000494:pharmacology; D006152:Cyclic GMP / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004578:Electron Spin Resonance Spectroscopy; D004837:Epinephrine / Q000494:pharmacology; D004912:Erythrocytes / Q000648:ultrastructure*; D005227:Fatty Acids / Q000032:analysis; D006160:Guanosine Triphosphate / Q000494:pharmacology; D006801:Humans; D008055:Lipids / Q000502:physiology; D008274:Magnesium / Q000494:pharmacology; D011458:Prostaglandins E / Q000494:pharmacology; D011506:Proteins / Q000502:physiology; D013113:Spin Labels; D013267:Stilbenes / Q000494:pharmacology; D013861:Thiocyanates / Q000494:pharmacology
D001557:Benzenesulfonates; D005227:Fatty Acids; D008055:Lipids; D011458:Prostaglandins E; D011506:Proteins; D013113:Spin Labels; D013267:Stilbenes; D013861:Thiocyanates; D006160:Guanosine Triphosphate; D002217:Carbachol; D000242:Cyclic AMP; D006152:Cyclic GMP; D008274:Magnesium; D004837:Epinephrine