title: Chlorophyll radical cation in photosystem II of chloroplasts. Millisecond decay at low temperature.
abstract: We compare the absorption changes, in the near infrared and in the green part of the spectrum, induced in spinach chloroplasts suspensions, at -- 170 degrees C, by continuous light and by flashes. (1) Following flash excitation, an absorption increase peaking at 825 nm which reverses rapidly (t 1/2 = 3.0 ms) is not affected by ferricyanide; it is suppressed when chloroplasts are preilluminated in the presence of 3-(3',4'-dichlorophenyl)-1,1'-dimethylurea (DCMU) and hydroxylamine. The reversion of that signal is simultaneous with a partial back reoxidation of C-550 (fully reduced by the flash) and with partial (about 25%) oxidation of cytochrome b559. The magnitude of the signal peaking at 825 nm (that we attribute to the radical cation of the trap chlorophyll of Photosystem II, acting as a primary electron donor) decreases progressively within a series of successive flashes. (2) An absorption increase (40% of which is slowly reversible) with a broad peak around 810 nm is induced by continuous light or by a flash. It is suppressed by pretreatment with ferricyanide, but it is little affected by the treatment with 3-(3',4'-dichlorophenyl)-1,1'-dimethylurea and hydroxylamine. We attribute it to oxidized P700. (3) With chloroplasts pretreated with 10 mM ferricyanide, an absorption increase, whose magnitude is nearly independent of wavelength between 790 and 870 nm, can be induced by continuous light. One saturating flash produces only 20% of the signal. This absorption change (20% of which is reversible in 30 s) might be due to a secondary donor of Photosystem II.
mesh_terms: D002734:Chlorophyll; D002736:Chloroplasts / Q000187:drug effects / Q000378:metabolism*; D003080:Cold Temperature; D003574:Cytochrome c Group / Q000378:metabolism; D003580:Cytochromes / Q000378:metabolism; D004237:Diuron / Q000494:pharmacology; D005292:Ferricyanides / Q000494:pharmacology; D005609:Free Radicals; D006898:Hydroxylamines / Q000494:pharmacology; D007700:Kinetics; D008027:Light; D010788:Photosynthesis; D010944:Plants / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D013055:Spectrophotometry, Infrared
chemical_list: D003574:Cytochrome c Group; D003580:Cytochromes; D005292:Ferricyanides; D005609:Free Radicals; D006898:Hydroxylamines; D002734:Chlorophyll; D004237:Diuron