Accelerated regeneration of trypsin-treated surface antigens of simian virus 40-transformed BALB/3T3 cells induced by X-irradiation.
The antigens of SV40-transformed BALB/3T3 cells measured by a radioisotopic footpad assay after removal by trypsin treatment regenerated in vitro in 3 to 6 hr. After X-irradiation with 3000 R, however, the antigens were regenerated to normal levels within 1 h. X-ray doses of between 1000 and 5000 R accelerated the regeneration of cell surface antigens, while X-irradiation with the larger dose of 8000 R did not. X-irradiation of nontrypsinized tumor cells was without effect. Possible mechanisms of this phenomenon are discussed.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis / Q000528:radiation effects*; D002462:Cell Membrane / Q000276:immunology; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology; D004307:Dose-Response Relationship, Radiation; D005260:Female; D005354:Fibrosarcoma / Q000276:immunology; D007114:Immunization; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009374:Neoplasms, Experimental / Q000209:etiology; D011830:Radiation Effects; D012038:Regeneration; D013539:Simian virus 40; D014357:Trypsin / Q000494:pharmacology; D014965:X-Rays
D000956:Antigens, Viral; D003513:Cycloheximide; D014357:Trypsin