Maturation pathway for Novikoff ascites hepatoma 5.8 S ribosomal ribonucleic acid. Evidence for its presence in 32 S nuclear ribonucleic acid.
Evidence that 32 S nRNA contains 5.8 S rRNA was provided by studies on specific oligonucleotide sequences of these RNA species. Purified 32P-labeled 5.8 and 28 S rRNA and 32 S RNA were digested with T-1 ribonuclease, and the products were fractionated according to chain length by chromatography on DEAE-Sephadex A-25 at neutral pH. The oligonucleotides in Peak 8 were treated with alkaline phosphatase and the products were separated by two-dimensional electrophoresis on cellulose acetate at pH 3.5 and DEAE-paper in 7% formic acid. Seven unique oligonucleotide markers for 5.8 S rRNA including the methylated octanucleotide A-A-U-U-Gm-G-A-Gp were present in 32 S RNA but were not found in 28 S rRNA, indicating that 5.8 S rRNA is directly derived from the 32 S nucleolar precursor. These studies confirm a maturation pathway for rRNA species in which 32 S nucleolar RNA is a precursor of 5.8 S rRNA as well as 28 S rRNA.
D000469:Alkaline Phosphatase; D000818:Animals; D001483:Base Sequence; D006528:Carcinoma, Hepatocellular / Q000032:analysis* / Q000378:metabolism; D002467:Cell Nucleus / Q000032:analysis*; D002852:Chromatography, Ion Exchange; D002854:Chromatography, Paper; D008113:Liver Neoplasms; D009374:Neoplasms, Experimental / Q000032:analysis / Q000378:metabolism; D009841:Oligonucleotides / Q000032:analysis; D010179:Pancreas / Q000201:enzymology; D010727:Phosphoric Diester Hydrolases; D012334:RNA, Neoplasm / Q000096:biosynthesis; D051381:Rats; D012260:Ribonucleases; D012265:Ribonucleotides / Q000032:analysis; D013154:Spleen / Q000201:enzymology
D009841:Oligonucleotides; D012334:RNA, Neoplasm; D012265:Ribonucleotides; D012260:Ribonucleases; D000469:Alkaline Phosphatase; D010727:Phosphoric Diester Hydrolases