Localization and quantitation of EBV-associated nuclear antigen (EBNA) in Raji cells.
Epstein-Barr virus (EBV)-associated nuclear antigen (EBNA) was localized to chromatin fibers of Raji cells. When EBNA was labeled with the horseradish-peroxidase technique of Sternberger a strong reaction covered most fiber surfaces as observed by electron microscopy. The dry mass of Raji chromatin increased after exposure to both EBV-positive and -negative serum, but the increase was significantly larger in the material treated with positive serum.
D000956:Antigens, Viral / Q000302:isolation & purification*; D002460:Cell Line; D002843:Chromatin / Q000382:microbiology; D003168:Complement Fixation Tests; D005455:Fluorescent Antibody Technique; D004854:Herpesvirus 4, Human / Q000276:immunology*; D006801:Humans; D007106:Immune Sera; D010544:Peroxidases / Q000276:immunology
D000956:Antigens, Viral; D002843:Chromatin; D007106:Immune Sera; D010544:Peroxidases