[Specificity of 3 alpha, 20 beta-hydroxysteroid: nad+ -oxidoreductase (author's transl)].
The enzyme 3 alpha, 20 beta-hydroxysteroid:NAD+ oxidoreductase (EC 1.1.1.53) catalyses the stoichiometric reduction of 20-oxo groups of C-21 steroids by NADH, and the non-stoichiometric oxidation of 3 alpha-hydroxy groups of 5 alpha-androstane derivatives by NAD. The activity of the enzyme towards the most important C-18, C-19 and C-21 steroids of human urine was tested in the presence of NAD and NADH. The rate of reduction of 20-oxo steroids is influenced by substituents at various positions of the steroid skeleton. These studies are intended as the basis of an eventual clinical chemical application.
D000736:Androstenes; D004796:Clinical Enzyme Tests; D003349:Cortisone Reductase / Q000378:metabolism*; D004963:Estrenes; D006801:Humans; D006913:Hydroxysteroid Dehydrogenases / Q000378:metabolism*; D009243:NAD; D011278:Pregnanes; D013256:Steroids / Q000652:urine; D013329:Structure-Activity Relationship
D000736:Androstenes; D004963:Estrenes; D011278:Pregnanes; D013256:Steroids; D009243:NAD; D006913:Hydroxysteroid Dehydrogenases; D003349:Cortisone Reductase
Untersuchungen über die Substratspezifität einer 3 alpha, 20 beta-Hydroxysteroid:NAD+-oxidoreductase