Non-T cell killing of mammary tumor cells by spleen cells: secretion of antibody and recruitment of cells.
Both T cell-mediated killing and non-T cell-mediated killing of target MTV-induced mammary tumor cells can be detected in microcytotoxicity assay tests of spleen cells from mice immunologically responsive to either the histocompatibility antigens or the virus-associated antigens of the target cells. The non-T cell-mediated cytotoxicity is antibody-dependent; otherwise inactive cells (null cells) can be recruited to activity by target cell-specific factors obtained from the supernatant of short-term cultures of sensitized B cells or provided by the introduction of a small number of sensitized B cells to the wells of the assay plate.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D000917:Antibody Formation; D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000378:metabolism; D002478:Cells, Cultured; D003601:Cytotoxicity Tests, Immunologic; D005260:Female; D007111:Immunity, Cellular; D066298:In Vitro Techniques; D008325:Mammary Neoplasms, Experimental / Q000276:immunology; D008324:Mammary Tumor Virus, Mouse / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D016038:Skin Transplantation; D013154:Spleen / Q000276:immunology*; D013601:T-Lymphocytes / Q000378:metabolism; D013997:Time Factors; D014184:Transplantation, Homologous
D000914:Antibodies, Viral