Influence of ionic strength of incubation medium on the release of some enzymes from rat liver mitochondria.
Influence of ionic strength of incubation medium on the release of adenylate kinase (AK), isocitrate dehydrogenase (IDH) and glutamate dehydrogenase (GDH) from intact and injured rat liver mitochondria has been studied. It has been found that raised ionic strength (mu equal 0.16-0.32) has increased the release of GDH and AK activities - IDH to a lesser degree - for mitochondrial preparations only with damaged inner membrane. Treatment of mitochondria with potassium chloride solution of definite ionic strength has permitted to detect the alteration of these organelles occurring in vivo and in vitro.
D000263:Adenylate Kinase / Q000302:isolation & purification* / Q000378:metabolism; D000818:Animals; D002251:Carbon Tetrachloride / Q000494:pharmacology; D002458:Cell Fractionation; D002499:Centrifugation, Density Gradient; D004072:Digitonin; D005969:Glutamate Dehydrogenase / Q000302:isolation & purification* / Q000378:metabolism; D007521:Isocitrate Dehydrogenase / Q000302:isolation & purification* / Q000378:metabolism; D008930:Mitochondria, Liver / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D009994:Osmolar Concentration; D010770:Phosphotransferases / Q000302:isolation & purification*; D011189:Potassium Chloride; D051381:Rats; D013010:Sonication
D011189:Potassium Chloride; D002251:Carbon Tetrachloride; D007521:Isocitrate Dehydrogenase; D005969:Glutamate Dehydrogenase; D010770:Phosphotransferases; D000263:Adenylate Kinase; D004072:Digitonin