Some characteristics of sulphate uptake into synaptosomes.
Incorporation of Na235SO4 by ovine brain Ficoll-prepared synaptosomes were studied as a function of time, temperature and substrate concentration. The rate of uptake obeyed Michaelis-Menten kinetics and apparent Km value of 4.4 mM Na2SO4 was determined. In the presence of p-hydroxymercuribenzoate a reduction in sulphate uptake was noted which suggests that thiol groups may possibly be implicated in the uptake mechanism.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002540:Cerebral Cortex / Q000378:metabolism*; D006902:Hydroxymercuribenzoates / Q000494:pharmacology; D007700:Kinetics; D012756:Sheep; D013431:Sulfates / Q000378:metabolism*; D013574:Synaptosomes / Q000187:drug effects / Q000378:metabolism*
D006902:Hydroxymercuribenzoates; D013431:Sulfates