title: [Mutagenic action of N-nitrosodimethylurea on Actinomyces rimosus and Penicillium chrysogenum].
abstract: High mutagenic activity of N-nitrozodimethylurea (NDMU), an agent of the group of the nitrozo compounds not studied in detail was shown with respect to prototrophic and auxotrophic strains of Actinomyces rimosus, an organism producing oxytetracycline and Penicillium chrysogenum, an organism producing penicillin. The rate of direct and back mutations in the auxotrophic strain of Act. rimosus under the effect of NDMU was many times higher than that of spontaneous mutations. NDMU was used at one of the selection stages at which a more active variant of Act. rimosus was obtained. This is evident of a possible use of the mutagen for induction of variation with respect to the quantitative feature of oxytetracycline production. A great number of morphologically changed forms and biochemical mutants of Pen. chrysogenum formed under the effect of this substance. NDMU induced a mutant of Pen. chrysogenum capable of selective synthesis of 6-aminopenicillinic acid without addition of the precursor.
mesh_terms: D014644:Genetic Variation / Q000187:drug effects; D008770:Methylnitrosourea / Q000031:analogs & derivatives / Q000494:pharmacology*; D009154:Mutation / Q000187:drug effects*; D009607:Nitrosourea Compounds / Q000494:pharmacology*; D010118:Oxytetracycline / Q000096:biosynthesis; D010397:Penicillanic Acid / Q000096:biosynthesis; D010406:Penicillins / Q000096:biosynthesis; D010407:Penicillium / Q000187:drug effects*; D010408:Penicillium chrysogenum / Q000187:drug effects* / Q000378:metabolism; D013171:Spores, Bacterial / Q000187:drug effects; D013172:Spores, Fungal / Q000187:drug effects; D013302:Streptomyces / Q000187:drug effects* / Q000378:metabolism
chemical_list: D009607:Nitrosourea Compounds; D010406:Penicillins; D008770:Methylnitrosourea; D010397:Penicillanic Acid; D010118:Oxytetracycline
vernacular_title: Mutagennoe deĭstvie N-nitrozodimetilmocheviny na Actinomyces rimosus i Penicillium chrysogenum