Growth of SV 40, adeno 7 and SV 40-adeno 7 viruses in monkey and human cells at 29 degrees and 37 degrees C.
Adeno 7 virus replicated well in human diploid (LEP) cells but only to a low degree in green monkey kidney (GMK) cells at 37 degrees C; it did not replicate in either system at 29 degrees C. At 37 degrees C SV 40 virus replicated well in GMK cells but only moderately in LEP cells; at 29 degrees C it did not replicate in either system. SV 40-adeno 7 hybrid grew in both GMK cells and LEP cells at 37 degrees C. At 29 degrees C this virus replicated in GMK cells but not in LEP cells. While the formation of V-antigen generally corresponded to the infectious virus production in the respective system, considerable differences were encountered in the T-antigens production. Adeno 7 T-antigen was detected earlier and in a higher percentage of GMK cells than in the fully permissive LEP cells and its formation was only slightly influenced by the incubation temperature. SV 40 T-antigen was more efficiently formed in GMK cells than in LEP cells. At 29 degrees C SV 40 T-antigen was only found in GMK cells and was detected later than at 37 degrees C. The difference in the formation of SV 40 T-antigen in GMK cells infected with SV 40 and SV 40-adeno 7 hybrid virus was further analyzed. The results obtained suggest that an early step of the virus-cell interaction, but neither virus attachment nor penetration, was involved.
D000256:Adenoviridae / Q000254:growth & development* / Q000276:immunology; D000327:Adsorption; D000818:Animals; D000951:Antigens, Neoplasm / Q000302:isolation & purification; D000956:Antigens, Viral / Q000302:isolation & purification; D002460:Cell Line; D002462:Cell Membrane / Q000382:microbiology; D005455:Fluorescent Antibody Technique; D000882:Haplorhini; D006801:Humans; D006824:Hybridization, Genetic; D007668:Kidney; D008168:Lung / Q000196:embryology; D013539:Simian virus 40 / Q000254:growth & development* / Q000276:immunology; D013696:Temperature; D014779:Virus Replication
D000951:Antigens, Neoplasm; D000956:Antigens, Viral