Determination of subgroup-specific feline oncornavirus-neutralizing antibody.
A microneutralization assay was developed for antibody-to-subgroup-specific feline oncornaviruses. This study combines the economic advantage of a microtiter system and the quantitative focus reduction method which permits contruction of multiplicity curves for determination of virus-neutralizing titers. A twofold increase in Synder-Theilen feline sarcoma virus (ST-FeSV) on feline embryo cells decreased by approximately twofold the titer of reference goat serum prepared against Kawakami-Theilen feline leukemia virus. Similar dose effects with FeLV serotype virus preparations were not observed. An assay system utilizing FeLV serotypes on sarcoma-positive leukemia-negative cells demonstrated slightly greater sensitivity than one employing ST-FeSV on FE cells. Differential antibody responses to the three subgroup-specific feline oncornaviruses (A, B ,and C) were observed in reference goat sera. This test demonstrated good reproducibility as well as sensitivity and constitutes a significant improvement over end point dilution assay systems.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D006041:Goats; D007106:Immune Sera; D009500:Neutralization Tests; D009858:Oncogenic Viruses / Q000276:immunology*; D005253:Sarcoma Viruses, Feline / Q000276:immunology* / Q000472:pathogenicity
D000914:Antibodies, Viral; D007106:Immune Sera