Separation of vitamin D metabolites and their analogues by high-pressure liquid chromatography.
The separation of vitamin D3 metabolites and their analogues by high-pressure liquid chromatography was investigated using a Zorbax SIL column. Each metabolite can be separated effectively with a solvent consisting of 2% methanol-methylene chloride or gradient elution from 0.02% to 6% methanol-methylene chloride. The C-24 epimers of 1alpha, 24-dihydroxy- and 1alpha,24,25-trihydroxy-vitamin D3 are resolved as their free forms, while the epimers of 24-hydroxy- and 24,25-dihydroxy-vitamin D3 can be separated as their trimethylsilyl derivatives.
D002762:Cholecalciferol; D002851:Chromatography, High Pressure Liquid; D004100:Dihydroxycholecalciferols / Q000032:analysis*; D006887:Hydroxycholecalciferols / Q000032:analysis*; D008722:Methods; D012997:Solvents; D014297:Trimethylsilyl Compounds
D004100:Dihydroxycholecalciferols; D006887:Hydroxycholecalciferols; D012997:Solvents; D014297:Trimethylsilyl Compounds; D002762:Cholecalciferol