A sensitive radiochemical assay for angiotensin-converting enzyme (kinase II).
[[125I]Tyr8]Bradykinin is degraded by angiotensin-converting enzyme to [125I]Tyr-Arg. The reaction product can be separated completely and recovered nearly quantitatively from unchanged substrate by cation-exchange chromatography. Thus it is possible to use [[125I]Tyr8]bradykinin at high specific radioactivity (about 400Ci/mmol) to measure the small quantities of angiotensin-converting enzyme encountered in small-scale cultures of pulmonary endothelial cells.
D000818:Animals; D001920:Bradykinin; D007457:Iodine Radioisotopes; D007700:Kinetics; D008168:Lung / Q000201:enzymology; D008722:Methods; D007703:Peptidyl-Dipeptidase A / Q000032:analysis* / Q000378:metabolism; D013552:Swine
D007457:Iodine Radioisotopes; D007703:Peptidyl-Dipeptidase A; D001920:Bradykinin