Alkali-labile sites and post-irradiation effects in gamma-irradiated biologically active double-stranded DNA in aqueous solution.
Gamma-irradiation of double-stranded RF-DNA of bacteriophage phi X174 in aqueous solution in the presence of oxygen produces at least one type of alkali-labile site. It is lethal and gives rise to breaks by alkali and is identical with the damage which becomes manifest by post-irradiation heat treatment. The effect of alkali is dependent on temperature. Furthermore, the excision repair system is not involved in eliminating lethal nucleotide damage in RFI-DNA.
D000468:Alkalies / Q000494:pharmacology; D010584:Bacteriophage phi X 174 / Q000187:drug effects; D004247:DNA / Q000528:radiation effects*; D004260:DNA Repair; D004277:DNA, Single-Stranded; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D010100:Oxygen; D013696:Temperature
D000468:Alkalies; D004277:DNA, Single-Stranded; D004247:DNA; D010100:Oxygen