title: [Enzymatic characteristics of the catalytic subunit of the protein kinase complex of human lymphocytes].
abstract: In earlier reports we have shown the existence in human lymphocytes homogenate, of a cyclic-AMP dependent protein-kinase activity. We demonstrate by affinity chromatography that two subunits display respectively cyclic-AMP binding and phosphorylating properties. Divalent cations such as Ca++, Mg++ or Mn++ are required for enzymatic activity. ATP which is an obligatory cosubstrate acts as an inhibitor when its concentration is higher than 10(-6)M.
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D000494:Allosteric Regulation; D000495:Allosteric Site; D001665:Binding Sites; D002846:Chromatography, Affinity; D003035:Cobalt / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation; D006801:Humans; D007526:Isoelectric Point; D007700:Kinetics; D008214:Lymphocytes / Q000201:enzymology*; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases / Q000097:blood*
chemical_list: D047428:Protein Kinase Inhibitors; D003035:Cobalt; D008345:Manganese; D000255:Adenosine Triphosphate; D000242:Cyclic AMP; D011494:Protein Kinases; D008274:Magnesium
vernacular_title: Caractéristiques enzymatiques de la sous-unité catalytique dans le complexe de la protéine-kinase du lymphocyte humain