[Studies on the control of IgM-antibody synthesis V. Affinity of anti-DNP antibodies in carps immunized with DNP-ficoll (author's transl)].
The dynamics of affinity of anti-DNP antibodies of carps immunized with T cell independent DNP-Ficoll was studied during the immune response. The intrinsic affinity (KO) of antibodies to monovalent epsilon-DNP-lysine is 10(5)--10(6)M-1 and does not change significantly in course of the immunisation. The affinity of tetrameric IgM antibodies to multivalent DNP-T4 conjugate is only 10(2)--10(3)-fold greater than to monovalent hapten, contrary to about 10(6)-fold higher values following immunisation with T cell-dependent antigens. The functional affinity (KF) increases during immune response slightly. KO and KF or antibodies are dependent on antigen dose. High antigen doses elicite antibodies with higher affinity.
D000818:Animals; D000915:Antibody Affinity; D000917:Antibody Formation; D002347:Carps; D004140:Dinitrophenols / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D007075:Immunoglobulin M / Q000032:analysis*; D008239:Lysine; D013601:T-Lymphocytes / Q000276:immunology
D004140:Dinitrophenols; D007075:Immunoglobulin M; D008239:Lysine
Untersuchungen zur Regulation der IgM-Immunantwort. V. Affinität von Karpfen-anti-DNP-Antikörpern nach Immunisierung mit DNP-Ficoll.