Poliovirus-induced infectious double-stranded RNA: Effect of RNA-degrading enzymes.
The infectivity of replicative form RNA (RF-RNA) isolated from poliovirus-infected HeLa cells is completely resistant to the action of T-1 RNase but decreases after exposure to RNase A in the presence of 0.3 M NaCl. Under these conditions neither enzyme produces single-stranded nicks in RF-RNA. Three endonuclease-free exonuleases (RNase II, polynucleotide phosphorylase and spleen phosphodiesterase) rapidly destroy the infectivity of single-stranded RNA, but do not alter the infectivity of RF-RNA. It is concluded that RF-RNA does not contain single-stranded ends essential for infectivity. Indirect evidence suggests that all or most of the poly A region at the 3' end of the plus strand of infectious RF-RNA is base-paired to a poly U region at the 5 end of the minus strand.
D001483:Base Sequence; D002499:Centrifugation, Density Gradient; D003090:Coliphages / Q000201:enzymology; D004720:Endonucleases / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D009691:Nucleic Acid Denaturation; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D010761:Phosphorus Radioisotopes; D017955:Poliovirus / Q000032:analysis / Q000254:growth & development; D011119:Polynucleotides / Q000032:analysis; D011117:Polyribonucleotide Nucleotidyltransferase / Q000378:metabolism; D012367:RNA, Viral / Q000032:analysis / Q000378:metabolism; D012260:Ribonucleases / Q000378:metabolism; D013154:Spleen / Q000201:enzymology; D014316:Tritium; D014688:Venoms; D010948:Viral Plaque Assay; D014779:Virus Replication
D010761:Phosphorus Radioisotopes; D011119:Polynucleotides; D012367:RNA, Viral; D014688:Venoms; D014316:Tritium; D011117:Polyribonucleotide Nucleotidyltransferase; D004720:Endonucleases; D012260:Ribonucleases; D010727:Phosphoric Diester Hydrolases