A new fluorogenic substrate for plasmin.
A new fluorogenic peptide substrate for plasmin, 7-(N-succinoylalanylphenylalanyl-lysylamido)-4-methylcoumarin trifluoroacetate salt, was prepared that can be used in a simple and direct assay. The results obtained by the assay method are linear over a wide range of enzyme concentrations and sensitive enough to detect as little as 10(-5) CTA units of plasmin. By making use of the inhibitor Trasylol and the differences in kinetic constants, plasmin can be specifically assayed even in the presence of the plasminogen activator thrombin, as well as in culture fluids from HeLa cells.
D003374:Coumarins / Q000138:chemical synthesis; D005341:Fibrinolysin / Q000378:metabolism*; D006367:HeLa Cells / Q000201:enzymology; D006801:Humans; D006868:Hydrolysis; D007700:Kinetics; D008722:Methods; D009842:Oligopeptides / Q000138:chemical synthesis; D010960:Plasminogen Activators / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013917:Thrombin / Q000378:metabolism; D014568:Urokinase-Type Plasminogen Activator / Q000378:metabolism
D003374:Coumarins; D009842:Oligopeptides; D010960:Plasminogen Activators; D013917:Thrombin; D005341:Fibrinolysin; D014568:Urokinase-Type Plasminogen Activator