title: The influence of nerve growth factor, potassium depolarization and dibutyryl (cyclic) adenosine 3',5'-monophosphate on explant cultures of chick embryo sympathetic ganglia.
abstract: Optimal nerve growth factor (NGF) and potassium concentrations for the culture of explants of single chick embryo sympathetic ganglia in Leighton tubes are described. NGF increases ganglionic monoamine oxidase (MAO; E.C.1.4.3.4) activity in a dose dependent fashion with maximum effects at a concentration of about 8 U/ml NGF. Peak activity compares closely with values seen in chick ganglia in vivo. Potassium-induced depolarization (45 mM K-+) also increases MAO activity; but the extent of the increase depends upon (NGF concentration, for there is little or no increase at high NGF concentrations. Dibutyryl adenosine 3',5'-cyclic monophosphoric acid (db cyclic AMP) can mimic the potassium-induced increase in MAO.
mesh_terms: D000818:Animals; D003994:Bucladesine / Q000494:pharmacology*; D002452:Cell Count; D002642:Chick Embryo; D046508:Culture Techniques; D004347:Drug Interactions; D005725:Ganglia, Autonomic / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D006652:Histological Techniques; D008564:Membrane Potentials; D008995:Monoamine Oxidase / Q000378:metabolism; D009414:Nerve Growth Factors / Q000378:metabolism / Q000494:pharmacology*; D011188:Potassium / Q000494:pharmacology*; D014316:Tritium; D014439:Tyramine / Q000378:metabolism
chemical_list: D009414:Nerve Growth Factors; D014316:Tritium; D003994:Bucladesine; D008995:Monoamine Oxidase; D011188:Potassium; D014439:Tyramine