title: Establishment of two canine sarcoma cell lines: productive infection with feline leukemia virus.
abstract: Two canine sarcoma cell lines (11028, 11031) were established in vitro and have been transferred 213 and 306 times, respectively, since 1970. These cell lines had a chromosome pattern consistent with their canine origin. Both cultures were infected with feline leukemia virus (FeLV), which caused a morphologic and karyotypic changes. The cells became rounded after infection with FeLV and both cultures showed the presence of a single, large, acrocentric chromosome considered to be a marker chromosome. All tumors were transplanted into newborn beagle pups, but only the 11028-FeLV formed metastatic tumors. No helper or focus-forming activity or virus particles were found in the uninfected cultures. Helper activity and virus were demonstrated in both sarcoma lines after infection with FeLV, though no focus-forming activity was noted. Helper activity of progeny virus could be assayed on either cat or dog embryo cells.
mesh_terms: D000818:Animals; D002415:Cats; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000473:pathology; D002875:Chromosomes / Q000648:ultrastructure; D004285:Dogs; D006378:Helper Viruses / Q000302:isolation & purification; D005256:Leukemia Virus, Feline / Q000254:growth & development; D009362:Neoplasm Metastasis; D012190:Retroviridae / Q000648:ultrastructure; D012513:Sarcoma, Experimental / Q000209:etiology / Q000235:genetics / Q000382:microbiology / Q000473:pathology