Influence of auxiliary enzymes on the spectrophotometric measurement of alanine aminotransferase and aspartate aminotransferase activities.
We investigated the enzyme activity of the blank in the spectrophotometric determination of the aminotransferase activities and aspartate aminotransferase activity. 6 lactate dehydrogenase and 3 malate dehydrogenase preparations from different manufactures and from different organs showed additional and contaminating activity. The additional activity depends upon the 2-oxoglutarate concentration. The contaminating activity is caused by alanine aminotransferase and aspartate aminotransferase in the auxiliary enzymes. We propose that exact definitions must be given for the auxiliary enzymes in the recommendations of standard determinations for enzyme activities.
D000410:Alanine Transaminase / Q000032:analysis*; D000645:Ammonium Sulfate; D001219:Aspartate Aminotransferases / Q000032:analysis*; D004355:Drug Stability; D005189:False Positive Reactions; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D009243:NAD; D011732:Pyridoxal Phosphate; D013053:Spectrophotometry / Q000379:methods
D009243:NAD; D011732:Pyridoxal Phosphate; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000645:Ammonium Sulfate