[Production of urea during mixed leukocyte culture (MLC). Effect of presensitization by a skin allograft].
An increased production of urea has been demonstrated during mixed cultures between allograft donor and recipient leukocytes. This phenomenon may also be observed during primary MLC (i.e, without previous allograft) but to a lesser degree. This increased production of urea during MLC results from an increase of arginase activity in spleen cell population(s) resulting in an increased arginine transformation into urea and ornithine, as demonstrated by the release of an amount of ornithine equivalent to that of urea in the culture supernatant.
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D007962:Leukocytes / Q000276:immunology / Q000378:metabolism*; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D016038:Skin Transplantation; D014184:Transplantation, Homologous; D014508:Urea / Q000378:metabolism*
D000596:Amino Acids; D014508:Urea
Production d'urée au cours des cultures mixtes leucocytaires (MLC). Effet d'une présensibilisation par une allogreffe de peau.