Recovery of transmissible gastroenteritis virus from chronically infected experimental pigs.
Transmissible gastroenteritis (TGE) virus was reisolated from pulmonary and intestinal tissues from 6 of 9 chronically infected experimental pigs (principals) necropsied 30 to 104 days after inoculation. Tissue homogenates (lung and small intestine) from the principals were prepared and inoculated into 3- to 5-day-old gnotobiotic pigs. The virus reisolated from the tissue homogenates produced a milder disease on 1st passage and a more severe disease on 2nd passage. The chronically infected experimental pigs (principals) developed serum-neutralization titers to TGE of 1:30 to 1:525. There appeared to be no relationship between serum titers and reisolation of TGE virus from the 9 principals. The persistence of virus in lung or intestine to 104 days indicates the recovered (or carrier) pig may be considered the primary source of TGE virus infection.
D000818:Animals; D003332:Coronaviridae / Q000302:isolation & purification*; D005761:Gastroenteritis, Transmissible, of Swine / Q000382:microbiology* / Q000473:pathology; D005856:Germ-Free Life; D007422:Intestines / Q000382:microbiology / Q000473:pathology; D008168:Lung / Q000382:microbiology / Q000473:pathology; D013552:Swine; D005760:Transmissible gastroenteritis virus / Q000302:isolation & purification*