Patterns of organization of actin and myosin in normal and transformed cultured cells.
The patterns of distribution of intracellular actin and myosin were examined by specific immunofluorescence in a series of normal, simian-virus-40-transformed, and revertant cell lines of rat and mouse origin. A consistent correlation was found between sensitivity to anchorage-dependent growth control and the presence of large, thick sheaths of actin-containing material. The presence of these sheaths was temperature-dependent in a rat line transformed by a temperature-sensitive mutant in the complementation group A of the oncogenic virus simian virus 40.
D000199:Actins / Q000032:analysis*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured / Q000032:analysis*; D005347:Fibroblasts; D005455:Fluorescent Antibody Technique; D009218:Myosins / Q000032:analysis*; D013539:Simian virus 40
D000199:Actins; D009218:Myosins