title: [Comparison of the sensitivity of laboratory animals and tissue cultures to infection with Aujeszky's disease virus].
abstract: In mice and guinea-pigs high susceptibility was demonstrated following i. c. administration of the virus, approximately equalling to that of rabbits and tissue cultures. Also a relatively high susceptibility of guinea-pigs was demonstrated, with very distinct clinical symptoms of the disease, as compared with mice and rats after the other manners of infection. On the basis of the results obtained white mice were utilized for routine diagnostic of Aujeszky disease. After i. c. administration of positive, virus-containing material encephalitis develops with a rapid exitus occurring 12-24 hours earlier than in the rabbits. In brain tissue antigen was always demonstrated by immunofluorescence examination. Along with the biological experiment replicas of pig organs were examined by immunofluorescence method. Maximum of positive yellow-green fluorescence is found in the cytoplasma of epithelial tonsillar cells, sporadically in the cell nuclei. Epithelial cells are deformed according to the infection degree. The amount of antigen in the brain tissue is not so pronounced as in the tonsillar tissue, yet in animals with clinical pathological symptoms the antigen was always demonstrated.
mesh_terms: D000818:Animals; D000917:Antibody Formation; D000956:Antigens, Viral / Q000032:analysis; D046508:Culture Techniques; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D006564:Herpesviridae / Q000472:pathogenicity*; D011558:Herpesvirus 1, Suid / Q000302:isolation & purification / Q000472:pathogenicity*; D051379:Mice; D011557:Pseudorabies / Q000276:immunology* / Q000635:transmission; D011817:Rabbits; D051381:Rats; D013045:Species Specificity; D013552:Swine / Q000276:immunology
chemical_list: D000956:Antigens, Viral
vernacular_title: Porovnání vnímavosti laboratorních zvírat a tkánových kultur k infekci virem Aujeszkého choroby