Inhibition of pyrimidine incorporation without inhibition of DNA synthesis.
A putative lymphocytic chalone was tested measuring the incorporation of purine and pyrimidine nucleosides and by cytophotometry. The pyrimidine precursors were inhibited but not the purines. Thymidine and deoxycytidine incorporation even performed simultaneously with cytophotometry can be misleading in the analysis of the inhibition of cell division.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis*; D006131:Growth Inhibitors / Q000494:pharmacology; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000378:metabolism; D051379:Mice; D009694:Nucleic Acid Precursors / Q000378:metabolism*; D010835:Phytohemagglutinins / Q000494:pharmacology; D011684:Purine Nucleosides / Q000378:metabolism; D011741:Pyrimidine Nucleosides / Q000378:metabolism*
D006131:Growth Inhibitors; D009694:Nucleic Acid Precursors; D010835:Phytohemagglutinins; D011684:Purine Nucleosides; D011741:Pyrimidine Nucleosides; D004247:DNA