title: Formation of sulfhydryl groups in the walls of Eimeria stiedai and E. tenella oocysts subjected to in vitro excystation.
abstract: Eimeria stiedai or Eimeria tenella oocysts were incubated in aqueous cysteine hydrochloride (cysHCl) under carbon dioxide (CO2), aqueous cysHCl under air, water under CO2 or water under air, and analyzed for sulfhydryl (-SH) groups. The cysHCl-CO2 treatment produced more -SH groups than the other treatments and was effective in allowing activation of intact and sodium hypochlorite (NaOCl)-treated E. stiedai oocysts as well as NaOCl-treated E. tenella oocysts. The CO2-cysHCl complex may act directly on the oocyst wall, especially in the micropylar region, to unmask lipid-shielded disulfide bridges, which are reduced to -SH groups. The reduction apparently disturbs the protein superstructure of the oocyst wall, promotes opening of the micropyle, and changes the impermeable state of the sporulated oocyst.
mesh_terms: D000818:Animals; D001646:Bile; D002245:Carbon Dioxide / Q000494:pharmacology; D003545:Cysteine / Q000494:pharmacology; D004539:Eimeria / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D006997:Hypochlorous Acid / Q000494:pharmacology; D013438:Sulfhydryl Compounds / Q000096:biosynthesis*; D014357:Trypsin / Q000494:pharmacology
chemical_list: D013438:Sulfhydryl Compounds; D002245:Carbon Dioxide; D006997:Hypochlorous Acid; D014357:Trypsin; D003545:Cysteine