Depression of in vitro responsiveness to phytohemagglutinin in spleen cells cultured from chickens with Marek's disease.
Cultures of dispersed spleen cells, prepared from MDV-infected chickens with MD visceral lymphomas, showed marked depression of responsiveness to the T cell mitogen PHA, as measured by 3H-Tdr incorporation in cells in vitro. When data are expressed quantitatively in terms of cpm/10(5) viable cells, the functional depletion of PHA-responsive cells appear to result from lower levels of 3H-Tdr incorporation in the PHA-stimulated spleen cultures from chickens with acute MD symptoms, as compared to similar cultures from uninfected isolator-reared control chickens. It is suggested that depression of PHA-induced blastogenesis is spleen cell cultures from chickens with acute MD reflects virus-related alterations in T lymphocytes.
D000362:Agar; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis; D002478:Cells, Cultured; D002645:Chickens; D005241:Feathers; D006564:Herpesviridae; D008381:Herpesvirus 2, Gallid / Q000276:immunology; D007165:Immunosuppression Therapy; D037102:Lectins; D008213:Lymphocyte Activation; D008380:Marek Disease / Q000276:immunology*; D011233:Precipitin Tests; D013154:Spleen / Q000166:cytology; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014422:Turkeys; D014611:Vaccination; D014765:Viral Vaccines
D000914:Antibodies, Viral; D000956:Antigens, Viral; D037102:Lectins; D014765:Viral Vaccines; D014316:Tritium; D000362:Agar; D013936:Thymidine