DNA-mediated genetic changes in Neurospora crassa.
Evidence for genetic transformation in Neurospora crassa is based on the observations that allo-DNA has a specific effect in producing transformants which is abolished by DNAase treatment and that iso-DNA is not effective in transformation. Here, unambiguous evidence for genetic transformation is provided by transfer of a temperature-sensitive inositol requirement from a donor to a recipient strain. Data provided also suggest the role of growth conditions and the involvement of a nuclease gene in the DNA uptake and transformation of N. crassa.
D004271:DNA, Fungal / Q000378:metabolism*; D003851:Deoxyribonucleases / Q000378:metabolism; D007294:Inositol / Q000378:metabolism; D009154:Mutation; D009491:Neurospora / Q000235:genetics*; D009492:Neurospora crassa / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D013696:Temperature; D014170:Transformation, Genetic
D004271:DNA, Fungal; D007294:Inositol; D003851:Deoxyribonucleases