Comparative characteristics of three cytomegaloviruses from rodents.
One cytomegalovirus isolate from the vole (Microtus arvalis) and 2 cutomegalovirus strains from mice (Apodemus sylvatious and Mus musculus) were compared. The rates of inactivation of these 3 viruses were virtually identical after each of 3 treatments: Repeated cycles of freezing and thawing, ultrasonic vibration, and exposure to trypsin and chymotrypsin. The replication of the 3 viruses was similar in each of 3 types of mouse embryo fibroblast cells tested. A number of other characteristics of the vole isolate were the same as those of the mouse strains, including buoyant density (1.20 g/cu cm), enhanced plaquing efficiency in diethylaminoethyl-dextran, and induction of an abortive replication cycle in human cells. The 3 viruses were indistinguishable with regard to their antigenic characteristics, and electron microscopic examination revealed that the nucleocapsids and enveloped virions of the 3 viruses were indistinguishable.
D000818:Animals; D000956:Antigens, Viral; D003411:Arvicolinae / Q000382:microbiology*; D002918:Chymotrypsin / Q000494:pharmacology; D003587:Cytomegalovirus / Q000254:growth & development / Q000276:immunology; D005615:Freezing; D051379:Mice / Q000382:microbiology*; D012377:Rodentia / Q000382:microbiology*; D014357:Trypsin / Q000494:pharmacology; D010948:Viral Plaque Assay
D000956:Antigens, Viral; D002918:Chymotrypsin; D014357:Trypsin