Effects of follicle-stimulating hormone on cultures of Sertoli cell preparations.
Sertoli cell-enriched preparations were obtained by sequential enzyme treatment of testes of 18-20 day old rats, and were maintained in culture in a chemically defined medium. The addition of highly purified follicle-stimulating hormone (FSH) to cells immediately after preparation, or after 48 h in culture, elicited an increase in the level of cyclic adenosine 3',5'-monophosphate (cAMP) when incubated in the presence of 3-isobutyl-1-methylxanthine (MIX). Luteinizing hormone (LH) had no effect on the cAMP levels. The cells cultured in the presence of FSH or dibutyryl cAMP for 48 h incorporated more [3H]leucine into trichloroacetic acid (TCA)-insoluble material than did cells cultured in the basal medium. Cycloheximide abolished the amino acid incorporation into protein precipitated by TCA. The data demonstrate that the Sertoli cell is a target cell for FSH action, and indicate that added dibutyryl cAMP can duplicate the enhancement of amino acid incorporation into protein elicited by FSH.
D000818:Animals; D002449:Cell Aggregation; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D007930:Leucine / Q000378:metabolism; D007986:Luteinizing Hormone / Q000494:pharmacology; D008297:Male; D008854:Microscopy, Electron; D014176:Protein Biosynthesis / Q000187:drug effects; D051381:Rats; D012708:Sertoli Cells / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D012756:Sheep; D013997:Time Factors; D014970:Xanthines / Q000494:pharmacology
D014970:Xanthines; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D000242:Cyclic AMP; D007930:Leucine