Detection of virus-specific DNA and RNA base-sequences in individual cells transformed or infected by adenovirus type 2.
By means of 3H thymidine-labelled adenovirus 2 DNA, adenovirus-specific DNA sequences have been localized in individual nuclei of adenovirus 2- or 12-infected cells, and adenovirus-specific RNA sequences have been detected in the permissive cells as well as in cells transformed by adenovirus type 2. The ability to detect such specific virus RNA base-sequences in particular, by in situ hybridization, should be useful in studying the transcriptional specificities of tumours and neoplasms.
D000256:Adenoviridae; D000257:Adenoviridae Infections / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D004279:DNA, Viral / Q000032:analysis*; D006801:Humans; D006824:Hybridization, Genetic; D012367:RNA, Viral / Q000032:analysis*; D051381:Rats
D004279:DNA, Viral; D012367:RNA, Viral