Isolation of membrane-bound renal kallikrein and kininase.
Fractions highly enriched in plasma membrane, endoplasmic reticulum or brush border were prepared from homogenized rat kidney cortex. Kallikrein was concentrated in the plasma-membrane fraction, but not in the brush border of the proximal tubules. Kininase II or angiotensin I-converting enzyme was localized in the brush-border membrane. It is suggested that kallikrein in the urine may originate from the plasma membrane of the distal tubules and the conversion of angiotensin I and the inactivation of bradykinin may occur on the lumen membrane of the proximal tubular cells.
D000818:Animals; D002462:Cell Membrane / Q000032:analysis; D007610:Kallikreins / Q000302:isolation & purification* / Q000378:metabolism; D007672:Kidney Cortex / Q000032:analysis*; D008297:Male; D007703:Peptidyl-Dipeptidase A / Q000302:isolation & purification* / Q000378:metabolism; D051381:Rats
D007703:Peptidyl-Dipeptidase A; D007610:Kallikreins