[Study of Aujeszky's disease virus replication by using an immunofluorescent method].
Studied was the interrelationship between the Aujeszky's disease virus (the avirulent mutant strain MK and the virulent strain 2) and chick embryo cells during cultivation with and without 5-iod-2-desoxiuridine. In experiments with the virulent strain the presence of a granular antigen could be observed at the 4th hour following the infection of the nucleus. After the 10th hour the antigen showed migration from the nucleus toward the cytoplasm. After the 24th hour a complete destruction of the cellular monolayer was observed, along with the production of polynuclear cells. In the experiments with strain MK the antigen seemed to appear first in the cytoplasm after the 10th hour of infecting the cells, and after the 12th hour it was found in the nucleoplasm too. In this case full destruction of the cells set in after the 48th hour of infection. In the cultivation of strain MK in cell cultures containing 5-iod-2-desoxiuridine no disturbances in the normal cycle of reproduction was observed. The virus replicated equally well both with and without the antimetabolite. In the cultivation of the virulent strain 2 in cell cultures that contained 5-iod-2-desoxiuridine a coarse, granular antigen was noticed in the cytoplasm of single cells after the eighth hour of infection. After the 12th hour the nuclei of these cells manifested slight flourescence (hardly visible single granules). After the 48th hour the nuclei of the involved cells were swollen, and the cytoplasm presented slight, diffuse fluorescence, some of the cells being destroyed.
D000818:Animals; D000956:Antigens, Viral; D002478:Cells, Cultured; D002642:Chick Embryo; D005455:Fluorescent Antibody Technique; D006564:Herpesviridae / Q000502:physiology*; D011558:Herpesvirus 1, Suid / Q000502:physiology*; D007065:Idoxuridine / Q000494:pharmacology; D013997:Time Factors; D014774:Virulence; D014776:Virus Cultivation; D014779:Virus Replication / Q000187:drug effects
D000956:Antigens, Viral; D007065:Idoxuridine
Prouchvane replikatsiiata na virusa na bolestta na Aueski s pomoshta na imunofluorestsentniia metod