The interaction between d-tubocurarine, pancuronium, polymyxin B, and neostigmine on neuromuscular function.
The interaction between pancuronium, d-tubocurarine, polymyxin B, and neostigmine was studied in the rat diaphragm-phrenic nerve preparation. Polymyxin B (5 mug/ml) did not affect twitch tension alone but decreased the pancuronium ED50 from 0.8 mug/ml to 0.32 mug/ml and the d-tubocurarine ED50 from 0.25 mug/ml to 0.15 mug/ml. Neostigmine (0.2 to 10 mug/ml) antagonized pancuronium or d-tubocurarine-induced depression of twitch tension. In contrast, neostigmine (0.001 to 0.2 mug/ml) augmented polymyxin B depression. Similarly, neostigmine (0.1 mug/ml) augmented combined polymyxin B-pancuronium or polymyxin B-d-tubocurarine depression of twitch tension. The authors conclude that polymyxin B potentiates the neuromuscular blockade from pancuronium or d-tubocurarine and that neostigmine further augments this block.
D000818:Animals; D003964:Diaphragm / Q000294:innervation; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects; D009388:Neostigmine / Q000008:administration & dosage / Q000494:pharmacology*; D009407:Nerve Block; D009469:Neuromuscular Junction / Q000187:drug effects; D010197:Pancuronium / Q000008:administration & dosage / Q000494:pharmacology*; D010791:Phrenic Nerve / Q000187:drug effects; D011113:Polymyxins / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D014403:Tubocurarine / Q000008:administration & dosage / Q000494:pharmacology*
D011113:Polymyxins; D009388:Neostigmine; D010197:Pancuronium; D014403:Tubocurarine