Activation of rat liver pyrimidine nucleoside monophosphate kinase.
The activity of the pyrimidine nucleoside monophosphate kinase (ATP:dCMP phosphotransferase, EC 2.7.4.14) from rat liver is dependent upon the presence of sulfhydryl-reducing agents. Addition to the inactive enzyme of 2-mercaptoethanol (5 mM), a reagent specific for cleavage of disulfide bonds, effects a reduction in molecular weight from approx. 53 000 to 17 000, measured by molecular sieve chromatography. This low molecular weight form is partially active in the presence of 2-mercaptoethanol (f mM). In absence of 2-mercaptoethanol, the low molecular weight form is inactive. Higher concentrations of 2-mercaptoethanol (50 mM) fully reactivate the CMP(ATP) kinase activity followed by dCMP(ATP) and CMP(dCTP) kinase activities in a sequential manner, without further change in moelcular weight. Alkylation by iodoacetamide of the enzyme at different stages of reactivation in dithiothreitol suggests an ordered appearance of the various enzyme activities. Furthermore, iodoacetamide inactivates the fully active enzyme. Thioredoxin was found to activate the enzyme in a manner similar to 2-mercaptoethanol and dithiothreitol. These results are consistent with the interpretation that the mechanism of activation of the enzyme involves cleavage of inter- and intramolecular disulfide bonds.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D003597:Cytosine Nucleotides / Q000494:pharmacology; D004229:Dithiothreitol / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D009703:Nucleoside-Phosphate Kinase; D010770:Phosphotransferases / Q000378:metabolism*; D051381:Rats; D012492:Salts / Q000494:pharmacology; D013997:Time Factors
D003597:Cytosine Nucleotides; D012492:Salts; D000255:Adenosine Triphosphate; D010770:Phosphotransferases; C019979:cytidylate kinase; D009703:Nucleoside-Phosphate Kinase; D004229:Dithiothreitol