The production of foot-and-mouth disease virus from BHK 21 C 13 cells grown on the surface of glass spheres.
In view of the advantages which are associated with the use of the BHK monolayer cell for the production of foot-and-mouth disease (FMD) virus, a unit system using glass spheres was developed to grow BHK monolayer cells and to test the susceptibility of such cells to FMD virus. The yield of cells and their susceptibility compares favorably with GREEN BHK monolayer cells which have been grown in Roux bottles.
D000818:Animals; D005537:Aphthovirus / Q000254:growth & development*; D002455:Cell Division; D002460:Cell Line; D006224:Cricetinae; D005898:Glass; D007668:Kidney; D014776:Virus Cultivation / Q000379:methods*