title: Reversible inhibition of the motility of human spermatozoa by tetraphenylboron.
abstract: The motility of washed suspensions of human spermatozoa was completely inhibited by tetraphenylboron at concentrations that had little effect on sperm energy metabolism. The inhibition of motility was reversed by quaternary ammonium salts, albumin, caffeine, dibutyryl cyclic AMP and potassium ions. The addition of ouabain to cells redndered immotile by tetraphenylboron prevented reinitiation of motility by potassium but not by the other compounds. These observations, together with the effect of tetraphenylboron on the fluorescence of sperm suspensions treated with 1-anilinonaphthalene 8-sulphonic acid, suggest that the binding of tetraphenylboron to sites on the sperm plasma membrane is involved in the inhibition of sperm motility and the cyclic AMP may be involved in the regulation of ion transport across the plasma membrane.
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis; D000418:Albumins / Q000494:pharmacology; D000817:Anilino Naphthalenesulfonates / Q000494:pharmacology; D001896:Boron Compounds / Q000494:pharmacology*; D002110:Caffeine / Q000494:pharmacology; D002452:Cell Count; D000242:Cyclic AMP / Q000494:pharmacology; D006019:Glycolysis / Q000187:drug effects; D006584:Hexamethonium Compounds / Q000494:pharmacology; D006801:Humans; D066298:In Vitro Techniques; D007773:Lactates / Q000032:analysis; D008297:Male; D010042:Ouabain / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000378:metabolism; D013775:Tetraphenylborate / Q000037:antagonists & inhibitors / Q000494:pharmacology*
chemical_list: D000418:Albumins; D000817:Anilino Naphthalenesulfonates; D001896:Boron Compounds; D006584:Hexamethonium Compounds; D007773:Lactates; D002110:Caffeine; D013775:Tetraphenylborate; D010042:Ouabain; D000255:Adenosine Triphosphate; D000242:Cyclic AMP; D011188:Potassium