[A micromethod for determination of the adenylate cyclase activity by chromatography on silufol UV-254 plates].
A method is developed for micro-scale separation of the products of enzymatic degradation of ATP (ADP, 5'-AMP, adenosine 3',5'-cytophosphate, adenosine and adenine) by one-dimentional thin-layer chromatography on Silufol UV-254 plates. The method is applicable for determination of activity of adenylate cyclase and of other enzymes involved in metabolism of adenylic derivatives, including the diesterase of 3',5'-cyclic AMP.
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000032:analysis; D000262:Adenylyl Cyclases / Q000032:analysis*; D002855:Chromatography, Thin Layer / Q000379:methods; D008832:Microchemistry; D014466:Ultraviolet Rays
D015105:3',5'-Cyclic-AMP Phosphodiesterases; D000262:Adenylyl Cyclases
Ispol'zozovanie silufola UF-254 dlia mikroopredeleniia adenilattsiklaznoĭ aktivnosti