Evidence for tandem integration of avian myeloblastosis virus DNA with endogenous provirus in leukemic chicken cells.
The integration site of avian myeloblastosis virus (AMV) proviral DNA in DNA from leukemia chicken myeloblasts has been studied by three sequential nucleic acid hybridizations that can localize the proviral DNA according to the repetitiveness of the adjacent cellular DNA regions. First, large denatured cellular DNA fragments (2.1 x 10(6) daltons) were reassociated and fractionated according to sequence reiteration frequenct. Next, DNA remaining single-stranded in each fraction was immobilized on nitrocellulose filters hybridized with an excess of unlabeled 70S RNA from Rous-associated virus-0 to saturate the endogenous proviral DNA sequences.
D000818:Animals; D001354:Avian Leukosis Virus / Q000378:metabolism*; D009189:Avian Myeloblastosis Virus / Q000378:metabolism*; D002645:Chickens; D002874:Chromosome Mapping; D004247:DNA / Q000032:analysis; D004279:DNA, Viral / Q000378:metabolism*; D007938:Leukemia / Q000235:genetics / Q000662:veterinary; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D014779:Virus Replication / Q000187:drug effects*
D004279:DNA, Viral; D004247:DNA