Rapid purification of lactate dehydrogenase X from mouse testes by two steps of affinity chromatography on oxamate-sepharose.
At concentrations of 200 muM NADH and 0.5 M NaCl LDH-X is separated from the other LDH isozymes of mouse testes on oxamate-sepharose. In a second step LDH-X is bound to the same matrix at lower NADH and NaCl concentrations and the pure enzyme can subsequently be eluted.
D000818:Animals; D002846:Chromatography, Affinity; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000302:isolation & purification* / Q000378:metabolism; D008297:Male; D051379:Mice; D009243:NAD / Q000378:metabolism; D013737:Testis / Q000201:enzymology
D007527:Isoenzymes; D009243:NAD; D007770:L-Lactate Dehydrogenase