[Estradiol receptors in the sex organs and some other organs of male rats].
A study was made of specific binding of estradiol (E2) by cytosol and the extracts of the nuclei of the testes, the prostate, the kidneys and the liver of the sexually-mature male rats. In the cytosol of all the mentioned organs there were found macromolecules with a high affinity (Kac similar 1--4-10(9)M(-1)). Low capacity ti the hormone (Nc similar to (1--2)--minus 14 mol per 1 mg of protein) and a marked hormonal sterospecificity--only the preparations with an estrogenic activity, but not testosterone, 5alpha-dihydrotestosterone, progesterone and corticosterone competed for H-3--E2 binding. The capacity of cytosol of these organs to bind E2 apecifically was eliminated by pronase, but not by RNA-ase and DNA-ase. The mentioned properties of the E2-binding macromolecules of cytosol of the testes, the prostate, the kidneys and the liver permitted to refer them to the estrogenic protein-receptors. Specific E2-binding was also revealed in the extracts of the nuclei of these organs, but its level was extremely low.
D000818:Animals; D001667:Binding, Competitive; D002467:Cell Nucleus / Q000378:metabolism; D003345:Corticosterone / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism; D013196:Dihydrotestosterone / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D007668:Kidney / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D046911:Macromolecular Substances; D008297:Male; D011374:Progesterone / Q000378:metabolism; D011402:Pronase / Q000378:metabolism; D011467:Prostate / Q000378:metabolism*; D051381:Rats; D011956:Receptors, Cell Surface; D012260:Ribonucleases / Q000378:metabolism; D013737:Testis / Q000378:metabolism*; D013739:Testosterone / Q000378:metabolism; D014599:Uterus / Q000378:metabolism
D046911:Macromolecular Substances; D011956:Receptors, Cell Surface; D013196:Dihydrotestosterone; D013739:Testosterone; D011374:Progesterone; D004958:Estradiol; D003851:Deoxyribonucleases; D012260:Ribonucleases; D011402:Pronase; D003345:Corticosterone
Retseptory k éstradiolu v organakh polovoĭ sfery i nekotorykh drugikh organakh samtsov krys