Isolation of 4 alpha-methyl-5 alpha-ergosta-8,24(28)-dien-3 beta-ol from ester fractions of nutritionally deprived Neurospora crassa.
A method of isolating pure fractions of 4 alpha-methyl-5 alpha-ergosta-8,24(28)-dien-38-ol for sterol intermediate studies is described. Starvation cultures of Neurospora crassa readily incorporate exogenous mevalonic acid into the sterol ester fraction. Isolation involves a simple solvent extraction and two chromatograms. Only the ester fraction yielded the required purity. Radioactive 4 alpha-methyl-5 alpha-ergosta-8,24(28)-dien-3 beta-ol is readily produced from DL-[2-14C] mevalonic acid.
D002775:Cholestadienols / Q000096:biosynthesis* / Q000302:isolation & purification; D003470:Culture Media; D007536:Isomerism; D008798:Mevalonic Acid / Q000378:metabolism; D009491:Neurospora / Q000378:metabolism*; D009492:Neurospora crassa / Q000032:analysis / Q000378:metabolism*
D002775:Cholestadienols; D003470:Culture Media; C023800:4 alpha-methyl-5 alpha-ergosta-8,24(28)-dien-3 beta-ol; D008798:Mevalonic Acid