title: Effect of Triton X-100 on the hydrolysis of sphingomyelin by sphingomyelinase of rat brain.
abstract: Mixed dispersions of the nonionic detergent Triton X-100 and sphingomyelin were used as substrate for sphingomyelinase of rat brain. The dependence of the rate of hydrolysis on the concentration of sphingomyelin was measured in two ways: at a fixed concentration of Triton X-100 or at varying concentrations of this detergent, while maintaining a fixed molar ratio of Triton X-100 to sphingomyelin. In either case, the upsilon vs. S curves deviated from the hyperbolic shape predicted by the Michaelis-Menten kinetic theory. These deviations are discussed and interpreted on the basis of the physicochemical properties of the mixed dispersions of detergent and lipid studied in previous papers.
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology*; D007700:Kinetics; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000494:pharmacology*; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology*; D051381:Rats; D013108:Sphingomyelin Phosphodiesterase / Q000378:metabolism*
chemical_list: D000644:Quaternary Ammonium Compounds; D011092:Polyethylene Glycols; D010727:Phosphoric Diester Hydrolases; D013108:Sphingomyelin Phosphodiesterase