title: Ammonium sulfate precipitation as a tool for the study of androgen receptor proteins in rat prostate and mouse kidney.
abstract: Ammonium sulfate precipitation has been used for the separation of bound and free steroids in rat prostate and mouse kidney cytosol equilibrated with tritiated androgens. A high affinity, low capacity binding protein has been identified in the 35% saturation precipitate. Biochemical and physiological data indicate that this protein is identical with the previously described 8-10 S androgen receptor. It has been demonstrated that this receptor protein binds 17 beta - hydroxy-5alpha-androstan-3-one (DHT) and testosterone in both tissues. The apparent dissociation constant (Kd) of the prostatic receptor for DHT and of the renal receptor for testosterone is 1-2 nM. The number of binding sites equals 57 and 23 fmoles/mg protein in prostate and kidney respectively. Dterminations of apparent inhibition constants (Ki) for 26 steroidal and non-steroidal compounds suggest that the binding sites in these tissues is similar or identical.
mesh_terms: D000645:Ammonium Sulfate; D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D011232:Chemical Precipitation; D003600:Cytosol / Q000378:metabolism; D013196:Dihydrotestosterone / Q000378:metabolism*; D005260:Female; D007668:Kidney / Q000378:metabolism*; D008297:Male; D051379:Mice; D011467:Prostate / Q000378:metabolism*; D011485:Protein Binding; D011506:Proteins / Q000302:isolation & purification* / Q000378:metabolism; D051381:Rats; D011956:Receptors, Cell Surface; D013739:Testosterone / Q000378:metabolism*
chemical_list: D011506:Proteins; D011956:Receptors, Cell Surface; D013196:Dihydrotestosterone; D013739:Testosterone; D000645:Ammonium Sulfate