Alkylation of meseenger RNA by dimethylnitrosamine.
Rats were treated with radioactive dimethylnitrosamine or methyl methanesulphonate and the alkylation of various RNA fractions was determined 4 h later. It was found that after administration of dimethylnitrosamine, hepatic messenger RNA (isolated by binding to oligo(dT)-cellulose) and nuclear RNA were alkylated slightly, but significantly less than ribosomal RNA. After treatment with methyl methanesulphonate nuclear RNA was alkylated to a greater extent than either messenger or ribosomal RNA. The implications of these findings with respect to the mechanism by which dimethylnitrosamine inhibits liver protein synthesis are discussed.
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004128:Dimethylnitrosamine / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008698:Mesylates / Q000378:metabolism*; D008741:Methyl Methanesulfonate / Q000378:metabolism*; D009602:Nitrosamines / Q000378:metabolism*; D011687:Purines / Q000032:analysis; D011743:Pyrimidines / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000378:metabolism*; D051381:Rats
D008698:Mesylates; D009602:Nitrosamines; D011687:Purines; D011743:Pyrimidines; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D008741:Methyl Methanesulfonate; D004128:Dimethylnitrosamine