Rat hepatoma cells nucleolar DNA. 2. Analysis of nucleolar DNA.
The analysis by CsCl density gradient of nucleolar DNA has revealed that the 1.700 g/cm3 main component can be subdivided in three subcomponents with buoyant densities of 1.707 g/cm3, 1.700 g/cm3, 1.690 g/cm3. The 1.707 g/cm3 and 1.690 g/cm3 components contain all the repetitive sequences which comprise 15 % of the total nucleolar DNA. The ribosomal cistrons are found in components having buoyant density between 1.707 g/cm3 and 1.725 g/gm3. Sodium-p-aminosalicylate-DNA interactions have revealed that only the 1.700 g/cm3 fraction has a destabilized secondary structure. The possible localization of these different fractions on peri and intranucleolar fractions is discussed.
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000032:analysis*; D002466:Cell Nucleolus / Q000032:analysis*; D002467:Cell Nucleus / Q000032:analysis / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D004273:DNA, Neoplasm / Q000032:analysis*; D008113:Liver Neoplasms; D009374:Neoplasms, Experimental / Q000032:analysis; D009693:Nucleic Acid Hybridization; D009695:Nucleic Acid Renaturation; D051381:Rats; D012270:Ribosomes / Q000032:analysis; D013936:Thymidine / Q000378:metabolism
D004273:DNA, Neoplasm; D013936:Thymidine