The requirement for membrane sialic acid in the stimulation of superoxide production during phagocytosis by human polymorphonuclear leukocytes.
The effect of desialylation on phagocytosis of latex particles and oxidative metabolism of human polymorphonuclear leukocytes was studied. Removal of 20% total leukocyte sialic acid by bacterial neuraminidase had no effect on phagocytosis of latex particles and phagocytosis-associated activation of hexose monophosphate shunt in human polymorphonuclear leukocytes. In contrast, desialylation prevented the stimulation of superoxide production either by phagocytosis or by concanavalin A. It is concluded that membrane sialic acid is essential for the stimulation of superoxide production by human polymorphonuclear leukocytes.
D002462:Cell Membrane / Q000502:physiology; D003208:Concanavalin A / Q000494:pharmacology; D003574:Cytochrome c Group; D005947:Glucose / Q000378:metabolism; D006801:Humans; D009439:Neuraminidase; D009504:Neutrophils / Q000276:immunology* / Q000378:metabolism / Q000648:ultrastructure; D009580:Nitroblue Tetrazolium; D010084:Oxidation-Reduction; D010100:Oxygen / Q000096:biosynthesis*; D010587:Phagocytosis; D012794:Sialic Acids / Q000502:physiology*; D013481:Superoxides / Q000096:biosynthesis*
D003574:Cytochrome c Group; D012794:Sialic Acids; D003208:Concanavalin A; D013481:Superoxides; D009580:Nitroblue Tetrazolium; D009439:Neuraminidase; D005947:Glucose; D010100:Oxygen