Viral and cellular DNA synthesis in nuclei from human lymphocytes transformed by Epstein-Barr virus.
A DNA-synthesizing system in vitro, using nuclei prepared by treatment of human lymphocytes with the detergent Brij 58, was developed. Nuclei from cultured lymphocytes synthesized DNA for as long as 5 hr, and required ATP, deoxynucleoside triphosphates, magnesium, and a calcium chelator. In nuclei from a partially synchronized line of cultured lymphocytes carrying several hundred copies of the Epstein-Barr viral genome, synthesis in vitro was predominately viral in early S phase and cellular in late S phase. These and other data suggested that the DNA synthesis observed in vitro was predominately replicative.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D001973:Bromodeoxyuridine / Q000378:metabolism; D002458:Cell Fractionation; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D004247:DNA / Q000096:biosynthesis; D004279:DNA, Viral / Q000096:biosynthesis; D003902:Detergents / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D004854:Herpesvirus 4, Human; D006801:Humans; D007700:Kinetics; D037102:Lectins / Q000494:pharmacology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D008274:Magnesium / Q000494:pharmacology
D004279:DNA, Viral; D003902:Detergents; D037102:Lectins; D004533:Egtazic Acid; D000255:Adenosine Triphosphate; D004247:DNA; D001973:Bromodeoxyuridine; D008274:Magnesium