Immunocytochemical localization of simian virus 40 T antigen with peroxidase-labeled antibody fragments.
T antigen was localized in simian virus 40 lytically infected and transformed cells by Fab' antibody conjugates at the ultrastructural level. This virus-specific protein appeared in the cytoplasm of permissive cells as early as 3 h after infection. At later time intervals, the T antigen was localized in both the cytoplasm and nucleus and finally (24 h) in the nucleus. These results suggest a synthesis of T antigen on cytoplasmic ribosomes, with subsequent transfer to the nucleus.
D000951:Antigens, Neoplasm / Q000302:isolation & purification*; D000956:Antigens, Viral / Q000302:isolation & purification*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D046508:Culture Techniques; D005455:Fluorescent Antibody Technique; D007140:Immunoglobulin Fab Fragments; D010544:Peroxidases; D013539:Simian virus 40 / Q000276:immunology*
D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D007140:Immunoglobulin Fab Fragments; D010544:Peroxidases