title: Reactivity of avian RNA tumor viruses with lectins.
abstract: The infectivity of avian RNA tumor viruses was inactivated to varying degrees by treatment with either concanavalin A (Con A) or phytohemagglutinin but not by treatment with wheat germ agglutinin. In general, leukosis viruses reacted preferentially with Con A, whereas sarcoma viruses showed more affinity for phytohemagglutinin. In a more extensive study with subgroup A of Prague Rous sarcoma virus (PR-A), the effect of inactivation by Con A could be specifically prevented by the addition of alpha-methyl-D-mannoside, alpha-methyl-D-glucoside, and N-acetyl-D-glucosamine. These sugars were also capable of eluting [3H]glucosamine-labeled material from disrupted PR-A virus, which was bound to a Con A-sepharose affinity column. A major viral glycoprotein recovered from the column had the same mobility as gp85 in polyacrylamide gel electrophoresis and could be immunoprecipitated with anti-gp85 antiserum. These results suggest that the material reacting with Con A is present on the gp85 component of the viral glycoprotein. The diversity in the reactivity of the glycoproteins of transforming and nontransforming viruses with plant lectins is discussed.
mesh_terms: D000116:Acetylgalactosamine / Q000494:pharmacology; D000117:Acetylglucosamine / Q000494:pharmacology; D001355:Alpharetrovirus / Q000254:growth & development*; D001354:Avian Leukosis Virus / Q000032:analysis / Q000254:growth & development; D001358:Avian Sarcoma Viruses / Q000032:analysis / Q000254:growth & development; D001666:Binding Sites, Antibody; D002471:Cell Transformation, Neoplastic; D003208:Concanavalin A / Q000494:pharmacology; D046508:Culture Techniques; D014644:Genetic Variation; D006023:Glycoproteins / Q000032:analysis; D037102:Lectins / Q000494:pharmacology*; D008757:Methylglucosides / Q000494:pharmacology; D008766:Methylmannosides / Q000494:pharmacology; D037121:Plant Lectins; D014908:Triticum; D014675:Vegetables; D014764:Viral Proteins / Q000032:analysis; D014779:Virus Replication
chemical_list: D006023:Glycoproteins; D037102:Lectins; D008757:Methylglucosides; D008766:Methylmannosides; D037121:Plant Lectins; D014764:Viral Proteins; D003208:Concanavalin A; D000116:Acetylgalactosamine; D000117:Acetylglucosamine