title: An experimental inactivated vaccine against bluetongue.
abstract: Bluetongue virus grown in BHK cells was shown to be inactivated in concentrations of betapropiolactone (BPL)  higher than 0-15 per cent. When the virus, inactivated with 0-2 per cent BPL and prepared as a double emulsion vaccine, was injected into Cypriot sheep, no untoward reactions were observed and neutralising antibodies developed. The antibody titre reached a high level and persisted for at least a year. After re-vaccination, a secondary response was observed. A bivalent vaccine elicited a response to both virus types incorporated. The possibilities of using a polyvalent inactivated vaccine are discussed.
mesh_terms: D000818:Animals; D000917:Antibody Formation; D000956:Antigens, Viral / Q000008:administration & dosage; D001819:Bluetongue / Q000276:immunology / Q000517:prevention & control*; D001820:Bluetongue virus / Q000254:growth & development / Q000276:immunology*; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D007273:Injections, Intramuscular; D007274:Injections, Intraperitoneal; D007279:Injections, Subcutaneous; D007668:Kidney; D007783:Lactones / Q000494:pharmacology; D009500:Neutralization Tests; D011422:Propionates / Q000494:pharmacology; D012087:Reoviridae / Q000276:immunology*; D012756:Sheep; D014765:Viral Vaccines / Q000008:administration & dosage
chemical_list: D000956:Antigens, Viral; D007783:Lactones; D011422:Propionates; D014765:Viral Vaccines