Rapid identification of amino acid derivatives from the sequenator.
A simple procedure is described for converting thiazolinones, from the protein sequenator, to the more stable phenylthiohydantoins without using the conventional HCI conversion procedure. The thiazolinones are applied to a silica gel plate and converted to phenylthiohydantoins by heating at 140 for 5-10 min, in the presence of heptafluorobutyric acid, prior to chromatography. After chromatography, measurements of the yields of the derivatives on the plate can be made with a variable-wave-length thin-layer chromatography scanner. This is shown to be a useful adjunct for identifications.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis*; D001059:Apoproteins / Q000032:analysis; D002384:Catalysis; D002855:Chromatography, Thin Layer; D004355:Drug Stability; D005782:Gels; D006358:Hot Temperature; D009113:Muramidase / Q000032:analysis; D009211:Myoglobin / Q000032:analysis; D010669:Phenylthiohydantoin / Q000032:analysis; D012822:Silicon Dioxide; D013844:Thiazoles
D000596:Amino Acids; D001059:Apoproteins; D005782:Gels; D009211:Myoglobin; D013844:Thiazoles; D010669:Phenylthiohydantoin; D012822:Silicon Dioxide; D009113:Muramidase