title: Structure and function of herpesvirus genomes. I. comparison of five HSV-1 and two HSV-2 strains by cleavage their DNA with eco R I restriction endonuclease.
abstract: The restriction endonuclease Eco R I cleaves HSV-1 and HSV-2 DNA into specific fragments that can be resolved by agarose gel electrophoresis. Comparison of HSV-1 strains KOS, 14-012, MP, F, and CI 101, and HSV-2 strains 333 and 186, suggests that the DNAs from type 1 strains are similar but not identical, and that the type 2 strains differ greatly from type 1 strains. The molecular lengths of the fragments have been determined by electron microscopy and can be used to calibrate gel electrophoretic analyses of DNA fragments.
mesh_terms: D000362:Agar; D001345:Autoradiography; D004279:DNA, Viral; D004586:Electrophoresis; D004720:Endonucleases; D005796:Genes; D005827:Genetics, Microbial; D007242:Herpesvirus 1, Bovine; D008854:Microscopy, Electron; D008970:Molecular Weight; D018139:Simplexvirus
chemical_list: D004279:DNA, Viral; D000362:Agar; D004720:Endonucleases