title: [Network of interactions between unlinked genes: synergistic and antagonistic regulation of iso-1-cytochrome c, iso-2-cytochrome c and cytochrome b2 synthesis].
abstract: Five chromosomal genes, CYPI to CYP5 involved in the regulation of the synthesis of iso-1-cytochrome c, iso-2-cytochrome c and cytochrome b2 are described. The function of these genes was studied either by varying the proportion of the mutated and wild type alleles in the cell vy varing the growth conditions, or else by transforming the mutants into sigma-cytoplasmic petites. We have shown a network of genetic interactions which regulate the synthesis of three structurally different proteins : iso-1-cytochrome c, iso-2-cytochrome c and cytochrome b2, by two unlinked genes : CYC1 and CYP1, one of which (CYC1) is the structural gene by iso-1-cytochrome c. Within this network the interactions are proportional to the gene dosage and are either antagonistic or synergistic depending on the allele combination and the protein studied. The mutated alleles cyp1 stimulate the synthesis of iso-2-cytochrome c, inhibit the synthesis of iso-1-cytochrome c, while the cytochrome b2 synthesis is also inhibited but by a combination of cyp1 mutated alleles CYC1 wild type allele. Other loci, CYP2, CYP3, CYP4 and CYP5 were also studied in various allelic combinations. They show some interactions between them or with CYC1 locus but these interactions are different and less pronounced than those involving loci CYP1 and CYC1.
mesh_terms: D000483:Alleles; D003579:Cytochrome Reductases / Q000096:biosynthesis; D003574:Cytochrome c Group / Q000096:biosynthesis; D003580:Cytochromes / Q000096:biosynthesis*; D005809:Genes, Regulator; D008040:Genetic Linkage; D009154:Mutation; D010641:Phenotype; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D014170:Transformation, Genetic
chemical_list: D003574:Cytochrome c Group; D003580:Cytochromes; D003579:Cytochrome Reductases
vernacular_title: Réseau d'interactions entre des génes non liés : régulation synergique ou antagoniste de la synthèse de l'iso-1-cytochrome c, de l'iso-2-cytochrome c et du cytochrome b2