Purification and characterization of commercial NADH and accompanying dehydrogenase inhibitors.
The anion-exchange chromatography of commercial NADH using a potassium bicarbonate solution as eluent yields highly pure NADH with good stability. Twelve compounds are also separated which act as dehydrogenase inhibitors. The main impurities are further characterized. The compound mainly responsible for residual optical density in commercial NADH preparations is probably a stereoisomer of NADH which is in reversible equilibrium with NADH at pH values in the range 5-7. A method of thin-layer chromatography, to check commercial NADH preparations for impurities, is described.
D001639:Bicarbonates; D055598:Chemical Phenomena; D002621:Chemistry; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D009243:NAD / Q000302:isolation & purification*; D009247:NADH, NADPH Oxidoreductases / Q000037:antagonists & inhibitors*; D009904:Optical Rotation; D013057:Spectrum Analysis / Q000379:methods; D013237:Stereoisomerism
D001639:Bicarbonates; D009243:NAD; D009247:NADH, NADPH Oxidoreductases