The isolation of nuclei and basic nucleoproteins from the cellular slime mold Dictyostelium discoideum.
A method is described for the isolation of nuclei from an axenic strain of Dictyostelium discoideum using a sorbitol/Ficoll solution and low concentration of Triton X-100. Basic proteins have been extracted from the nuclei and on polyacrylamide gel electrophoresis yield a consistent pattern in which five major groups or bands predominate. Four of these five fractions comigrate with calf thymus histones and one fraction seems to be unique to D. discoideum. The slowest moving of the five fractions is soluble in 0.5 M perchloric acid and comigrates with calf thymus histone F1. After recovery from the perchloric acid solution by precipitation with acetone this fraction yielded one major band on electrophoresis.
D000818:Animals; D002417:Cattle; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000032:analysis; D003720:Densitometry; D004023:Dictyostelium / Q000032:analysis / Q000648:ultrastructure*; D004591:Electrophoresis, Polyacrylamide Gel; D005362:Ficoll; D006657:Histones; D009235:Myxomycetes / Q000648:ultrastructure*; D009698:Nucleoproteins / Q000302:isolation & purification*; D010472:Perchlorates; D011092:Polyethylene Glycols; D012995:Solubility; D013012:Sorbitol; D013950:Thymus Gland
D006657:Histones; D009698:Nucleoproteins; D010472:Perchlorates; D005362:Ficoll; D011092:Polyethylene Glycols; D013012:Sorbitol