title: Pleiotropic effects of a DNA adenine methylation mutation (dam-3) in Escherichia coli K12.
abstract: The dam-3 mutation results in a five-fold reduction in the number of 6-methyl-adenine (6-meA) residues in the DNA of E. coli K12 or phage lambda. The DNA of phage fd appears to be devoid of 6-meA when propagated on dam-3 bacteria. The phenotypic differences between dam-3 and dam+ bacteria include: (i) increased free phage in lysogenic dam-3 cultures, (2) increased sensitivity to methyl methanesulfonate (MMS), (3) inviability of dam-3 lex-I strains, (4) lower molecular weight of DNA in dam-3 bacteria in the absence of DNA ligase and (5) increased rate of DNA degradation in dam-3 recA strains.
mesh_terms: D000225:Adenine / Q000378:metabolism*; D002501:Centrifugation, Zonal; D003090:Coliphages / Q000378:metabolism; D003227:Conjugation, Genetic; D004260:DNA Repair; D004269:DNA, Bacterial / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D008242:Lysogeny; D008698:Mesylates / Q000494:pharmacology; D008745:Methylation; D008970:Molecular Weight; D009154:Mutation; D010641:Phenotype; D011831:Radiation Genetics; D014161:Transduction, Genetic; D014466:Ultraviolet Rays
chemical_list: D004269:DNA, Bacterial; D004279:DNA, Viral; D008698:Mesylates; D000225:Adenine