Derivation of mouse sarcoma virus (Kirsten) by acquisition of genes from heterologous host.
The technique of virus RNA-cellular DNA hybridization in solution with DNA excess was used to compared the nucleotide sequences of the 70 S RNA genome of the Kirsten mouse sarcoma virus (Ki-MSV) with that of mouse erythroblastosis virus (MEV) which gave rise to Ki-MSV after in vivo propagation in rat. It is suggested that a loss of about 30% of the genomic sequences of MEV with a concomitant gain of roughly equal amounts of rat-specific sequences in a genetically stable recombinant state led to the formation of Ki-MSV.
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D004247:DNA; D004273:DNA, Neoplasm; D017988:Gammaretrovirus / Q000032:analysis*; D005796:Genes; D009052:Leukemia Virus, Murine / Q000032:analysis / Q000254:growth & development; D051379:Mice; D009690:Nucleic Acid Conformation; D009711:Nucleotides / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis*; D051381:Rats; D009053:Sarcoma Viruses, Murine / Q000032:analysis* / Q000254:growth & development; D013045:Species Specificity
D004273:DNA, Neoplasm; D009711:Nucleotides; D012367:RNA, Viral; D004247:DNA