The influence of androgens on protein synthesis by cultured rat epididymal tubules.
Rat epididymal tubules maintained in organ culture for 3 days respond to the addition of androgens to the culture media (testosterone and dihydrotestosterone 1 x 10-minus 5 m and 1 x 10-minus 7 m) with an increased incorporation of amino acids into acid-insoluble material. Significant androgenic stimulation is observed only 24 h after addition of hormone, while an inhibitory effect is found at earlier periods. The stimulation seems to be specifically produced by androgens; it is blocked by cyproterone acetate and is not elicited by oestradiol-17beta or corticosterone. The process appears to involve RNA synthesis since actinomycin D suppresses the stimulatory effect of androgen. Evidence suggests that cAMP production is not a primordial step in the response to androgen since dibutyryl cAMP did not mimick the androgenic effect, theophylline did not potentiate the response and alpha,beta-methylene ATP, which competitively inhibits adenyl cyclase, failed to alter the androgenic effect. Radioactive testosterone and dihydrotestosterone added to the culture media showed a preferential intranuclear localization as well as extensive metabolism. DHT was found to be the principal intranuclear steroid.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001798:Blood Proteins / Q000378:metabolism; D003994:Bucladesine / Q000494:pharmacology; D000242:Cyclic AMP / Q000096:biosynthesis; D003609:Dactinomycin / Q000494:pharmacology; D003864:Depression, Chemical; D013196:Dihydrotestosterone / Q000494:pharmacology*; D004822:Epididymis / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D011485:Protein Binding; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis; D051381:Rats; D013268:Stimulation, Chemical; D013739:Testosterone / Q000494:pharmacology*; D013806:Theophylline / Q000494:pharmacology; D013997:Time Factors
D000596:Amino Acids; D001798:Blood Proteins; D013196:Dihydrotestosterone; D003609:Dactinomycin; D013739:Testosterone; D012313:RNA; D003994:Bucladesine; D000255:Adenosine Triphosphate; D013806:Theophylline; D000242:Cyclic AMP