Early events in the lytic infection of primary mouse kidney cell culture with polyoma virus. The effect of various input multiplicities.
Early polyoma (Py) virus-specific RNA synthesis was examined in cells infected with different concentrations of Py-virus. The effect of various multiplicities of infection (m.o.i.) on the rate of Py-RNA synthesis is different at 30 hr as compared to 65 hr. Thirty hours after infection at 27degrees, in the presence of 5-fluoro-2-deoxyuridine (FdU), an increase in input multiplicity was not associated with a quantitatively commensurate increase in the amount of virus-specific RNA synthesized. At 65 hr, the amount of viral RNA synthesized was roughly proportional to the number of infecting virus particles.
D002478:Cells, Cultured; D007700:Kinetics; D011120:Polyomavirus / Q000378:metabolism*; D012367:RNA, Viral / Q000096:biosynthesis*; D013997:Time Factors; D014779:Virus Replication
D012367:RNA, Viral