Synthesis of fibroma viral deoxyribonucleic acid complexes in rabbit kidney cells.
Cytoplasmic extracts of primary rabbit kidney cells inoculated with fibroma virus revealed 2 peaks of DNA complexes (120S and greater than or equal to 410S) in a linear sucrose gradient. Pulse-chase experiments demonstrated a shift in the gradient profile of lighter complexes toward heavier complexes. Synthesis of DNA complexes was inhibited by adding puromycin or actinomycin D. The DNA from virus-infected cultures hybridized 7 to 9 times greater with fibroma virus DNA than did the DNA from noninfected cultuures. The DNA complexes became increasingly resistant to deoxyribonuclease digestion as a function of time during viral growth cycle and produced tumors in rabbits.
D004279:DNA, Viral / Q000096:biosynthesis* / Q000302:isolation & purification; D003851:Deoxyribonucleases / Q000378:metabolism; D012776:Fibroma Virus, Rabbit / Q000378:metabolism* / Q000472:pathogenicity; D009693:Nucleic Acid Hybridization; D011212:Poxviridae / Q000378:metabolism*
D004279:DNA, Viral; D003851:Deoxyribonucleases