Measurement of parainfluenza-3 virus antibody by the single radial hemolysis technique.
A single radial hemolysis assay for parainfluenza-3 virus antibody is described as a simple, sensitive, accurate, and precise alternative to hemagglutination inhibition and serum neutralization tests. A highly significant correlation exists between single radial hemolysis zone areas and hemagglutination inhibition titers of both serum and nasal secretion samples. Antibody conversions equivalent to as little as a twofold rise in hemagglutination inhibition titer are readily and reliably detected in sera and nasal secretions. Single radial hemolysis and hemagglutination inhibition activity was demonstrable in the immunoglobulin G fraction of a hyperimmune parainfluenza-3 virus antiserum.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002417:Cattle; D005069:Evaluation Studies as Topic; D006385:Hemagglutination Inhibition Tests; D006461:Hemolysis; D007074:Immunoglobulin G / Q000032:analysis; D009297:Nasal Mucosa / Q000378:metabolism; D010224:Parainfluenza Virus 3, Human / Q000276:immunology*; D018108:Respirovirus / Q000276:immunology*; D012698:Serologic Tests / Q000379:methods*
D000914:Antibodies, Viral; D007074:Immunoglobulin G