[Plaque characteristics of herpes mamillitis virus (HMV)].
The virus of the bovine herpes mammillitis (VHM) produces a homogeneous plaque population. The plaques are either round or have the form of a slightly irregular circle, cized 2--3 mm in diameter, with a semitranslucent center. The microscopic examination of paticular plaques has revealed a necrotic center composed of destroyed cells, and in cases where the gross examination gives a clearly defined ontour there is a band of both normal and destroyed cells. No morphologic differences have been established in plaque formation so far as local strains KOS/H-ovo and DZS and the reference strain TVA are concerned. It has been found that the number of the plaques formed is directly proportional to the concentration of the inoculated virus. The preciseness and specificity of the plaque reduction test has been demonstrated as well. The comparative testing of hydrolysed starch, agarose, agar, and carboxylmethylcellulose for the plaque study of VHM has shown that best results are obtained with the use of starch and agarose, no morphologic variation being established among plaques overlaid with the four tested substances.
D003588:Cytopathogenic Effect, Viral; D006564:Herpesviridae; D001911:Herpesvirus 2, Bovine
Plakova kharakteristika na virusa na kherpesniia mamilit (VHM)