title: Proteins specified by herpes simplex virus. XIII. Glycosylation of viral polypeptides.
abstract: In the course of herpes simplex virus 1 (HSV-1) replication in human epidermoid carcinoma no. 2 cells, the synthesis and glycosylation of host cell proteins ceases and is replaced by the synthesis and glycosylation of virus-specified polypeptides. Analyses of the synthesis of viral glycoproteins show that the glycosylation of viral polypeptides occurs late in the virus growth cycle and that certain of the precursors to major vital glycoproteins are members of the gamma group of polypeptides, i.e., polypeptides synthesized at increasing rates until 12 to 15 h postinfection. Viral glycoproteins are formed by stepwise additions of heterosaccharide chains to completed precursor polypeptides. The precursor and the highly glycosylated product are separable by gel electrophoresis and are localized in different fractions of infected cells. Within 15 min of their synthesis, precursor polypeptides acquire heterosaccharide chains of about 2,000 molecular weight, which contain glucosamine but little or nor fucose or sialic acid. Both precursor and product of this first stage of glycosylation are absent or present in low concentrations in the surface membranes of the infected cell and in the virion. The partially glycosylated product is then conjugated further in a slow, discontinuous process to form the mature glycoprotein of the virion and plasma membrane. These mature products bear large heterosaccharide units with molecular weights greater than 4,000 to 5,000; these contain fucose and sialic acid as well as glucosamine. Heterosaccharide chains from infected and uninfected cells are distributed among discrete size classes and the smallest chains consist of multiple saccharide residues.
mesh_terms: D002460:Cell Line; D002462:Cell Membrane / Q000032:analysis / Q000378:metabolism; D005643:Fucose / Q000032:analysis; D005944:Glucosamine / Q000032:analysis / Q000378:metabolism; D006020:Glycopeptides / Q000032:analysis / Q000096:biosynthesis; D006023:Glycoproteins / Q000096:biosynthesis*; D008970:Molecular Weight; D010455:Peptides / Q000378:metabolism; D011498:Protein Precursors / Q000378:metabolism; D011691:Puromycin / Q000494:pharmacology; D012794:Sialic Acids / Q000032:analysis; D018139:Simplexvirus / Q000032:analysis / Q000254:growth & development / Q000378:metabolism*; D014764:Viral Proteins / Q000032:analysis / Q000096:biosynthesis*; D014779:Virus Replication
chemical_list: D006020:Glycopeptides; D006023:Glycoproteins; D010455:Peptides; D011498:Protein Precursors; D012794:Sialic Acids; D014764:Viral Proteins; D005643:Fucose; D011691:Puromycin; D005944:Glucosamine