Stability of enzymes in starving Arthrobacter crystallopoietes.
Cell-free extracts prepared from spherical and rod-shaped cells of Arthrobacter crystallopoietes were assayed for enzymes during various periods of starvation. The level of NADH oxidase dropped to 20 and 30%, respectively, in spherical and rod-shaped cells during the first 1 to 2 days of starvation and then remained constant for 9 days. Catalase activity decreased continuously and reached a low level in 9 days. Enzymes involved in glucose metabolism and the tricarboxylic acid cycle were stable for the duration of the experiment (about 1 week). Succinic dehydrogenase, fumarase and aconitase were stable during 21 days of starvation, which is the longest time enzymes have been shown to be stable in any bacterium under conditions of total starvation.
D000154:Aconitate Hydratase / Q000378:metabolism; D001173:Arthrobacter / Q000201:enzymology* / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D002474:Cell-Free System; D004355:Drug Stability; D004798:Enzymes / Q000378:metabolism*; D006597:Fructose-Bisphosphatase / Q000378:metabolism; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism; D005649:Fumarate Hydratase / Q000378:metabolism; D005956:Glucose-6-Phosphate Isomerase / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D007521:Isocitrate Dehydrogenase / Q000378:metabolism; D008291:Malate Dehydrogenase / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D010734:Phosphogluconate Dehydrogenase / Q000378:metabolism; D013385:Succinate Dehydrogenase / Q000378:metabolism; D014050:Toluene / Q000494:pharmacology
D004798:Enzymes; D014050:Toluene; D008291:Malate Dehydrogenase; D007521:Isocitrate Dehydrogenase; D010734:Phosphogluconate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D002374:Catalase; D013385:Succinate Dehydrogenase; D009247:NADH, NADPH Oxidoreductases; D006597:Fructose-Bisphosphatase; D005634:Fructose-Bisphosphate Aldolase; D005649:Fumarate Hydratase; D000154:Aconitate Hydratase; D005956:Glucose-6-Phosphate Isomerase