Transformation of hamster kidney cells by BK papovavirus DNA.
Supercoiled BK papovavirus DNA was shown to transform hamster kidney cells using the calcium phosphate co-precipitation technique. The transformed cells contained intranuclear T-antigen(s) and rescuable virus and produced progressively growing tumors when inoculated into hamsters. A novel finding was the production in tumor-bearing animals of antinuclear antibody, which reacted against normal, untransformed cells; in addition, tumor serum contained antibody against virus-specific T-antigen(s).
D000818:Animals; D000974:Antibodies, Antinuclear / Q000032:analysis; D000912:Antibodies, Neoplasm / Q000032:analysis; D000951:Antigens, Neoplasm / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis; D001739:BK Virus / Q000254:growth & development / Q000276:immunology; D002130:Calcium Phosphates; D002459:Cell Fusion; D002460:Cell Line; D002467:Cell Nucleus / Q000276:immunology; D002471:Cell Transformation, Neoplastic; D011232:Chemical Precipitation; D006224:Cricetinae; D004279:DNA, Viral; D007668:Kidney; D009374:Neoplasms, Experimental / Q000276:immunology; D011120:Polyomavirus; D014779:Virus Replication
D000974:Antibodies, Antinuclear; D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D002130:Calcium Phosphates; D004279:DNA, Viral