Cytotoxic effects of activated human monocytes and lymphocytes to anti-D-treated human erythrocytes in vitro.
Incubation of human monocytes, derived from peripheral blood, with cell-free supernatants from mixed lymphocyte cultures resulted in morphological and functional changes in the mature macrophages. Activation of monocyte-derived macrophages by these factors resulted in a significant increase in their capacity to lyse anti-D-treated human erythrocytes. The lytic activity of both normal and activated macrophages appeared to be independent of erythrophagocytosis. T lymphocytes activated by either allogeneic cells or the mitogen phytohaemagglutinin were not cytolytic to treated erythrocytes even at high effector to target cell ratios.
D000906:Antibodies; D001402:B-Lymphocytes / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D004912:Erythrocytes / Q000276:immunology*; D006461:Hemolysis; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology*; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D008214:Lymphocytes / Q000276:immunology*; D008264:Macrophages / Q000276:immunology; D009000:Monocytes / Q000276:immunology*; D010587:Phagocytosis; D013601:T-Lymphocytes / Q000276:immunology
D000906:Antibodies; D007106:Immune Sera