title: The effect of octadecenoic acid on the growth of japanese encephalitis virus in Novikoff hepatoma cells.
abstract: The growth characteristics of Japanese encephalitis virus cultivated in Novikoff hepatoma cells grown in shaker culture can be differentially altered by the presence of 6-cis or cis-9-octadecenoic acid in Swim's 67-G medium. The addition of 125 mug of the 6-isomer per ml medium reduced the number of infectious particles produced, whereas the same amount of the 9-isomer enhanced virus production. The virus was found to be more stable in cell-free spent medium than in fresh medium. The presence of 125 mug 6-18:1 per ml in fresh medium resulted in a rapid loss of virus infectivity.
mesh_terms: D006528:Carcinoma, Hepatocellular; D002478:Cells, Cultured; D004664:Encephalitis Virus, Japanese / Q000187:drug effects / Q000254:growth & development*; D005231:Fatty Acids, Unsaturated / Q000494:pharmacology*; D008113:Liver Neoplasms; D009374:Neoplasms, Experimental
chemical_list: D005231:Fatty Acids, Unsaturated