Quantitative determination of poly(adenosine diphosphate ribose) in different hepatic tissues by an isotope dilution procedure.
A procedure has been developed for the quantitation of poly(ADP-ribose) in intact tissues. It is based on the dilution of added [3H]poly(ADP-ribose) by the endogenous polymer. 5 - 6 nanomoles protein-bound ADP-ribose per mg DNA were found in adult and neonatal rat liver, while Zajdela hepatoma cells had significantly lower values. A comparison with mono(ADP-ribose) residues in adult rat liver revealed similar levels of monomeric and polymeric ADP-ribose residues. This means that far more proteins (or acceptor sites on proteins) must be occupied by single ADP-ribose residues than by oligo or poly(ADP-ribose) chains. While the poly(ADP-ribose) levels of the different tissues do not correlate with the corresponding proliferation rates, the amount of mono(ADP-ribose) does show a certain Correlation, being low in rapidly growing tissues.
D000818:Animals; D000831:Animals, Newborn; D002286:Carcinoma, Ehrlich Tumor / Q000032:analysis; D006528:Carcinoma, Hepatocellular / Q000032:analysis*; D008099:Liver / Q000032:analysis*; D008113:Liver Neoplasms / Q000032:analysis*; D051379:Mice; D009374:Neoplasms, Experimental / Q000032:analysis; D009702:Nucleoside Diphosphate Sugars / Q000032:analysis*; D011064:Poly Adenosine Diphosphate Ribose / Q000032:analysis* / Q000302:isolation & purification; D011865:Radioisotope Dilution Technique; D051381:Rats; D014316:Tritium
D009702:Nucleoside Diphosphate Sugars; D014316:Tritium; D011064:Poly Adenosine Diphosphate Ribose