Estrogen-directed synthesis of specific prostaglandins in uterus.
Endogenous uterine prostaglandin levels were monitored in the cycling rat and prostaglandins of the F-type were found to rise at proestrus when estrogen levels have been shown to be maximal. Evidence that this is an estrogen-induced event is furnished by the finding that estradiol-17beta caused a similar rise in prostaglandin F levels in uteri of ovariectomized rats, an action blocked by coadministration of progesterone. Examination of in vitro prostaglandin synthetase by uterine microsomal fractions from cycling rats and estrogen-treated ovariectomized rats revealed that the action of estrogen to control prostaglandin synthesis in a directional manner is accomplished, at least in part, by regulation of the prostaglandin synthetase complex, resulting in a patterned alteration in the ratio of prostaglandin F to prostaglandin E. These results demonstrate that prostaglandins are involved in the expression of estrogen action in the rat uterus.
D000818:Animals; D002369:Castration; D000242:Cyclic AMP / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism; D004031:Diestrus; D004958:Estradiol / Q000494:pharmacology; D004967:Estrogens / Q000494:pharmacology*; D004971:Estrus; D005260:Female; D007213:Indomethacin / Q000494:pharmacology; D008861:Microsomes / Q000378:metabolism; D010053:Ovary / Q000502:physiology; D011247:Pregnancy; D011359:Proestrus; D011374:Progesterone / Q000494:pharmacology; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D011453:Prostaglandins / Q000096:biosynthesis*; D011460:Prostaglandins F / Q000096:biosynthesis; D051381:Rats; D013997:Time Factors; D014599:Uterus / Q000187:drug effects / Q000378:metabolism*
D004967:Estrogens; D011453:Prostaglandins; D011460:Prostaglandins F; D011374:Progesterone; D004958:Estradiol; D000242:Cyclic AMP; D011451:Prostaglandin-Endoperoxide Synthases; D006152:Cyclic GMP; D007213:Indomethacin