Kinetics of microcolonies of cultured mammalian cells after gamma irradiation.
The time of cell destruction was determined by pedigree analysis before macrocolony formation immediately after irradiation of gamma rays (400-900 R) in cultured rat hepatoma cells. Cell destruction occurred and was distributed to various generations in proportion to dose. The loss of reproductive integrity resulting in an extinction of a clone was limited in earlier generations, 0 to 2, but the loss of reproductive capacity was observed in later generations. It seems probable that two types of loss of reproduction come from random transmission of lethal signals produced in parent cells irrespective of the pattern of pedigrees.
D000818:Animals; D006528:Carcinoma, Hepatocellular; D002455:Cell Division / Q000528:radiation effects*; D002470:Cell Survival; D002999:Clone Cells; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D008113:Liver Neoplasms; D011829:Radiation Dosage; D011830:Radiation Effects; D051381:Rats; D013997:Time Factors