[Regulation of amylolytic complex enzyme biosynthesis in Endomycopsis fibuligera strain 20-9].
The effect of glucose and cyclic 3',5'-adenosine monophosphate (cAMP) on the synthesis of glucoamylase and alpha-amylase by the yeast Endomycopsis fibuligera str. 20-9 was investigated. The glucoamylase synthesis was inhibited by every concentration (0.05--3.0%) of glucose. The synthesis of alpha-amylase was significantly reduced by high concentrations (2.0-3.0%) of carbohydrate only. An addition of cAMP (1 X 10(-5) M) reversed compeltely the inhibitory effect of 0.5-2.0% glucose on the alpha-amylase synthesis and partially restored the glucoamylase synthesis. It is concluded that catabolite repression may be involved in the regulation of alpha-amylase and glucoamylase synthesis by Endomycopsis fibuligera str. 20-9 and that this type of regulation shows varying effectiveness in the enzyme synthesis.
D000681:Amylases / Q000096:biosynthesis*; D001203:Ascomycota / Q000201:enzymology*; D003470:Culture Media; D000242:Cyclic AMP / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005947:Glucose / Q000494:pharmacology; D005959:Glucosidases / Q000096:biosynthesis*; D009154:Mutation; D011134:Polysaccharides; D004718:Saccharomycetales / Q000187:drug effects / Q000201:enzymology*; D013997:Time Factors
D003470:Culture Media; D011134:Polysaccharides; D000242:Cyclic AMP; D000681:Amylases; D005959:Glucosidases; D005947:Glucose
Reguliatsiia biosinteza fermentov amiloliticheskogo kompleksa Endomycopsis fibuligera shtamm 20-9