title: In vitro action of gastric mucosal lysosomal enzymes on intracellular gastric glycoproteins.
abstract: The results show that incubation of gastric mucosal cells from rat at pH approximately 4.5 or in the presence of aspirin is associated with a specific increase in the activity of some acid-hydrolases. Intracellular glycoproteins, isolated by non-degrative techniques from rat or dog fundic mucosal cells, were found to be potential bio-substrates for these acid-hydrolyses. This may suggest that cleavage of the carbohydrate moieties of the intracellular and mucosal cell wall glycoproteins is a fundamental step in the development of gastric ulceration. A model for gastric lesions is proposed and discussed in the light of the results obtained.
mesh_terms: D000818:Animals; D001241:Aspirin / Q000494:pharmacology; D002241:Carbohydrates / Q000032:analysis; D004285:Dogs; D005643:Fucose; D005753:Gastric Mucosa / Q000201:enzymology*; D006020:Glycopeptides; D006023:Glycoproteins; D006026:Glycoside Hydrolases / Q000378:metabolism*; D008247:Lysosomes / Q000187:drug effects / Q000201:enzymology*; D051381:Rats; D013045:Species Specificity
chemical_list: D002241:Carbohydrates; D006020:Glycopeptides; D006023:Glycoproteins; D005643:Fucose; D006026:Glycoside Hydrolases; D001241:Aspirin