title: The molar ratio of the two major polypeptide components of human high density lipoprotein.
abstract: Human high density lipoprotein (HDL) and its subfractions (HDL2 and HDL3) were separated by ultracentrifugation and the molar ratio of the two major polypeptide chains apo-Gln-I and apo-Gln-II was determined by fluorescence tagging of sodium dodecyl sulfate-denatured proteins combined with polyacrylamide disc gel electrophoresis. Using purified apo-Gln-I and apo-Gln-II standards, it was found that holo HDL, holo HDL2, and holo HDL3 from all plasma samples contained a molar ratio of apo-Gln-I to the disulfide-bound dimer of apo-Gln-II of 2:1, that is a 1:1 ratio in terms of each species of polypeptide chain. The method described is useful for making repeated and rapid measurements on microgram quantities of intact lipoproteins.
mesh_terms: D001059:Apoproteins / Q000097:blood* / Q000302:isolation & purification; D001665:Binding Sites; D002241:Carbohydrates / Q000032:analysis; D004220:Disulfides / Q000032:analysis; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood*; D046911:Macromolecular Substances; D011485:Protein Binding; D013050:Spectrometry, Fluorescence
chemical_list: D001059:Apoproteins; D002241:Carbohydrates; D004220:Disulfides; D008075:Lipoproteins, HDL; D046911:Macromolecular Substances