Purification and partial characterization of different forms of phosphofructokinase in man.
Phosphofructokinase (ATP:D-fructose-6-phosphate 1-phosphotransferase, EC 2.7.1.11) from human muscle, brain, heart and granulocytes has been purified using a two or three step purification procedure. The main step is Blue Dextran-Sepharose 4B chromatography with selective elution of phosphofructokinase by formation of the ternary complex ADP or ATP-fructose-6-P-enzyme. Muscle and heart contain only enzyme subunits with a molecular weight of 85,000. This type of subunit is predominnant in brain, where it co-exists with subunits of about 80,000 daltons. A single type of subunits is found in the granulocytes, with a molecular weight of 80,000. Anti-muscle phosphofructokinase antiserum reacts only with M-type enzyme. Anti-granulocyte enzyme antiserum, absorbed by pure brain phosphofructokinase, exhibits a narrow specificity against the so-called L-type enzyme. Anti-brain antiserum, absorbed by pure muscle phosphofructokinase and partly purified red cell enzyme, exhibits a narrow specificity against a phosphofructokinase form predominant in fibroblasts and present in brain (F-type).
D001921:Brain / Q000201:enzymology; D006098:Granulocytes / Q000201:enzymology; D006801:Humans; D007106:Immune Sera; D005779:Immunodiffusion; D007527:Isoenzymes / Q000302:isolation & purification*; D008970:Molecular Weight; D009132:Muscles / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology; D010732:Phosphofructokinase-1 / Q000302:isolation & purification*; D011233:Precipitin Tests; D014018:Tissue Distribution
D007106:Immune Sera; D007527:Isoenzymes; D010732:Phosphofructokinase-1