Hepatic clearance of adenosine 3:5-cyclic monophosphate from plasma in the rat.
Rats were given intravenous injections of cyclic [3H]AMP and the disappearance of radioactivity from plasma and its appearance in bile were followed. Livers were removed and the cyclic [3H]AMP content was measured. The binding of radioactivity to soluble proteins was measured after preparations of a cytoplasmic fraction. Experiments in vitro to determine the ability of hepatic cytoplasmic proteins to bind cyclic [3H]AMP were also carried out. A role for cytoplasmic proteins in the clearance of cyclic AMP from plasma is discounted.
D000818:Animals; D000242:Cyclic AMP / Q000097:blood / Q000378:metabolism*; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism*; D008297:Male; D008657:Metabolic Clearance Rate; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism; D051381:Rats; D013997:Time Factors
D011506:Proteins; D000242:Cyclic AMP