[Use of snake venom immobilized phosphodiesterase for analysis of the nucleotide composition of oligodesoxyribonucleotides].
A simple one-step technique for the analysis of nucleotide composition of oligodeoxyribonucleotides is described. The method is based on enzymatic hydrolysis and subsequent separation of hydrolysate on a "combined" column with Aminex A 28 anionite, on which top immobilyzed on DEAE cellulose snake venom phosphodiesterase is layed. The method is tested using a number of synthetic di- and tri-deoxyribonucleotides.
D000818:Animals; D002384:Catalysis; D055598:Chemical Phenomena; D002621:Chemistry; D009841:Oligonucleotides; D009843:Oligoribonucleotides; D010727:Phosphoric Diester Hydrolases; D012910:Snake Venoms / Q000032:analysis*
D009841:Oligonucleotides; D009843:Oligoribonucleotides; D012910:Snake Venoms; D010727:Phosphoric Diester Hydrolases
Ispol'zovanie immobilizovannoĭ fosfodiésterazy zmeinogo iada dlia analize nukleotidnogo sostava oligodezoksiribonukleotidov