In vitro transformation of rat and mouse cells by DNA from simian virus 40.
Primary rat kidney cells and mouse 3T3 cells can be transformed by DNA of simian virus 40 when use is made of the calcium technique (Graham and van der Eb, 1973). The transformation assay in primary rat cells is reproducible, but the dose response is not linear.
D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis; D002130:Calcium Phosphates; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002499:Centrifugation, Density Gradient; D011232:Chemical Precipitation; D046508:Culture Techniques; D004270:DNA, Circular / Q000302:isolation & purification; D004279:DNA, Viral / Q000302:isolation & purification; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts; D005455:Fluorescent Antibody Technique; D007668:Kidney; D051379:Mice; D008807:Mice, Inbred BALB C; D051381:Rats; D013539:Simian virus 40 / Q000032:analysis / Q000276:immunology
D000951:Antigens, Neoplasm; D002130:Calcium Phosphates; D004270:DNA, Circular; D004279:DNA, Viral