A method for the gas chromatographic determination of vitamin D and related structures.
A procedure is described for the separation of Vitamin D and related compounds by gas-liquid chromatography using flame ionization detection. The method involves a two-step conversion: isomerization to the corresponding (all trans) isotachysterol(s) followed by methyl ether derivatization of the alcohol group(s). The procedure provides a means for identification as well as a possible basis for quantitation of Vitamin D and analogs.
D055598:Chemical Phenomena; D002621:Chemistry; D002762:Cholecalciferol / Q000032:analysis; D002849:Chromatography, Gas / Q000379:methods*; D002853:Chromatography, Liquid; D006887:Hydroxycholecalciferols / Q000032:analysis; D007536:Isomerism; D008722:Methods; D008738:Methyl Ethers / Q000032:analysis; D014807:Vitamin D / Q000031:analogs & derivatives / Q000032:analysis*
D006887:Hydroxycholecalciferols; D008738:Methyl Ethers; D014807:Vitamin D; D002762:Cholecalciferol