Evidence for 16alpha-hydroxylation of estrone 3-sulphate by guinea pig liver slices.
[6,7-3H,35S]Estrone 3-sulfate (E13S) of 3H/35S = 3.57 was incubated with female guinea pig liver slices. Small amounts of free steroid and estrone-3-glucuronide, each containing 3H, were found. In addition, E13S, 17beta-estradiol 3-sulfate, and a 'disulfate' fraction, with 3H/35S = 4.4, 4.3, and 4.7, respectively, were also isolated from the incubated tissue. The latter fraction was a major metabolite and about 45% of it consisted of 'disulfates' of 16alpha-hydroxyestrone and estriol, thus providing strong evidence for 16alpha-hydroxylation in guinea pig liver slices.
D000818:Animals; D004966:Estrogens, Conjugated (USP) / Q000378:metabolism; D004970:Estrone / Q000378:metabolism*; D005260:Female; D005965:Glucuronates / Q000378:metabolism; D006168:Guinea Pigs; D006900:Hydroxylation; D008099:Liver / Q000378:metabolism*; D039181:Steroid 16-alpha-Hydroxylase; D013464:Sulfuric Acids / Q000378:metabolism
D004966:Estrogens, Conjugated (USP); D005965:Glucuronates; D013464:Sulfuric Acids; D004970:Estrone; D039181:Steroid 16-alpha-Hydroxylase