Preparation of radioiodinated simian virus 40 DNA for use in DNA - DNA reassociation kinetics experiments.
A method is described for the preparation of 125I-labelled SV40 DNA. Using this method, SV40 DNA can be routinely labelled to 15 - 10(6) dpm per mug; much higher specific activities are easily obtained by minor modifications of the method. Once incorporated, the radioactive label dissociates from DNA exceedingly slowly at 4 degrees C or at 68 degrees C. Iodinated SV40 DNA is shown to be useful in the quantitation of viral nucleic acid sequences in SV40-transformed 3T3 cells by DNA - DNA reassociation kinetics.
D001483:Base Sequence; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D004279:DNA, Viral; D007457:Iodine Radioisotopes; D007700:Kinetics; D008722:Methods; D009695:Nucleic Acid Renaturation; D013539:Simian virus 40
D004279:DNA, Viral; D007457:Iodine Radioisotopes