Affinity chromatography purification of Clostridium perfringens enterotoxin.
Anti-enterotoxin immunoglobulins immobilized on CH-Sepharose or CNBr-Sepharose were used for affinity chromatography purification of Clostridium perfringens enterotoxin. Cell extracts containing enterotoxin or partially purified toxin preparations were applied to the column and nonspecifically-bound protein was eluted. NaOH was used to elute specifically bound toxin. The purity of enterotoxin purified by  Sephadex G-100 chromatography followed by affinity chromatography appears similar to toxin highly purified by conventional means. The procedure can be used  successfully for the rapid (less than 2 h) purification of small amounts of enterotoxin.
D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis; D002845:Chromatography; D002846:Chromatography, Affinity; D003016:Clostridium perfringens / Q000276:immunology*; D003488:Cyanogen Bromide; D003911:Dextrans; D004589:Electrophoresis, Disc; D004768:Enterotoxins / Q000302:isolation & purification*; D007106:Immune Sera; D005779:Immunodiffusion; D007834:Lasers; D008722:Methods; D011817:Rabbits / Q000276:immunology; D014020:Tissue Extracts
D001426:Bacterial Proteins; D003911:Dextrans; D004768:Enterotoxins; D007106:Immune Sera; D014020:Tissue Extracts; D003488:Cyanogen Bromide