Immunoreactive ACTH and cortisol plasma levels during pregnancy. Detection and partial purification of corticotrophin-like placental hormone: the human chorionic corticotrophin (HCC).
The high plasma cortisol and ACTH levels present in pregnant women as well as the non-parrallelism of their plasma extract dilution curves in comparison with the standard curve in the ACTH radioimmunoassay, are evidence for the presence of an ACTH-like substance during pregnancy which would interfere with the assay. Placental extracts were obtained by acid-acetone extraction, followed by partial purification with oxycellulose and by extraction with porous glass powder. A substance was detected which partially cross-reacted with synthetic human ACTH in the radioimmunoassay and which showed biological activity using the assay procedure described by Liscomb & Nelson. The data sustain the existence of an ACTH-like placental hormone: human chorionic corticotrophin (HCC).
D000324:Adrenocorticotropic Hormone / Q000097:blood* / Q000276:immunology / Q000302:isolation & purification*; D000818:Animals; D001681:Biological Assay; D003345:Corticosterone / Q000032:analysis; D003429:Cross Reactions; D046508:Culture Techniques; D005260:Female; D006801:Humans; D006854:Hydrocortisone / Q000097:blood*; D010926:Placental Hormones / Q000302:isolation & purification*; D011247:Pregnancy; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay; D051381:Rats
D010926:Placental Hormones; D000324:Adrenocorticotropic Hormone; D003345:Corticosterone; D006854:Hydrocortisone