Transformation of human embryonic fibroblasts by photodynamically inactivated herpes simplex virus, type 2 at supra-optimal temperature.
Infection of human embryonic fibroblast cell monolayers with neutral red and light-inactivated herpes simplex virus, type 2 (HSV-2) at supra-optimal temperature (42 degrees C) resulted in persistence of viable cells in suspension culture at 37 degrees C which have properties in common with virus transformed cells: formation of cell aggregates, HSV-2-specific antigens and colony formation in soft methyl cellulose medium. These data are consistent with the idea that photodynamic inactivated HSV-2 has potential oncogenic activity.
D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D005347:Fibroblasts; D006801:Humans; D008027:Light; D009499:Neutral Red / Q000494:pharmacology*; D010619:Phenazines / Q000494:pharmacology*; D018139:Simplexvirus / Q000187:drug effects / Q000276:immunology; D013696:Temperature
D000956:Antigens, Viral; D010619:Phenazines; D009499:Neutral Red