[Protein synthesis with cell extracts of Micrococcus radiodurans (author's transl)].
Pure active ribosomes of cells of Micrococcus radiodurans could be obtained when cultivated in trypton, glucose and nutrient broth by adding natrium citrate. The optimal conditions for a cell-free protein synthesis were investigated at the (polyuridylic acid) dependent polyphenylalanine synthesis. When exchanging ribosomes and S100-fractions with the corresponding fractions of E. coli, we found that the enzyme fractions of M. radiodurans extremely inhibit the ribosomal activity. The incorporation rates in the cell-free system of M. radiodurans yield, at comparable conditions, in relation to E. coli under 10%.
D001426:Bacterial Proteins / Q000096:biosynthesis*; D004926:Escherichia coli / Q000378:metabolism; D007700:Kinetics; D008837:Micrococcus / Q000378:metabolism*; D011072:Poly U; D014176:Protein Biosynthesis; D012270:Ribosomes / Q000378:metabolism; D013045:Species Specificity
D001426:Bacterial Proteins; D011072:Poly U
Proteinsynthese mit Zellextrakten von Micrococcus radiodurans.