Incorporation of nucleotides into DNA by mammalian DNA polymerase in the presence of a single deoxynucleoside triphosphate.
Rat ascites hepatoma cell DNA polymerases (EC 2.7.7.7), especially low molecular weight polymerase, could incorporate a significant amount of single nucleotide into acid-soluble products in the absence of the other three deoxynucleoside triphosphates when activated DNA was used as a template.  This relaxed requirement for deoxynucleotides was not observed when poly[d(A-T).d(T-A)] was used as a template.  Nearest-neighbour base analyses of the products formed in the presence of a single deoxynuclesode triphosphate revealed that the reaction is not of a terminal transferase-type but a very limited repair synthesis in which one or a few triphosphates are incorporated at numerous 3'-hydroxyl ends.
D000227:Adenine Nucleotides / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000201:enzymology; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis*; D004254:DNA Nucleotidyltransferases / Q000378:metabolism*; D004260:DNA Repair; D008113:Liver Neoplasms; D008722:Methods; D008970:Molecular Weight; D009711:Nucleotides / Q000378:metabolism; D010761:Phosphorus Radioisotopes; D011119:Polynucleotides / Q000378:metabolism; D051381:Rats; D013698:Templates, Genetic
D000227:Adenine Nucleotides; D009711:Nucleotides; D010761:Phosphorus Radioisotopes; D011119:Polynucleotides; D000255:Adenosine Triphosphate; D004247:DNA; D004254:DNA Nucleotidyltransferases