[Contribution of two-dimensional immunoelectrophoresis technics to the study of the structure and the morphogenesis of human adenovirus].
The two-dimensional immunoelectrophoresis has been applied to human adenovirus type-2 structural analysis. This method is both qualitative and quantitative and was used in the following studies. 1) Detection of major and minor capsid antigens. 2) Identification of viral antigenic components in the infected cell material. 3) Quantification of antigens and serological characterization of ts mutants. 4) Identification of polymeric forms of structural proteins. 5) Assembly analysis of capsid subunits. 6) Stoichiometric analysis of proteins within the virion. 7) Obtaining of monospecific sera.
D000260:Adenoviruses, Human / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D002213:Capsid / Q000032:analysis; D007123:Immunoelectrophoresis, Two-Dimensional; D009024:Morphogenesis; D009154:Mutation; D010641:Phenotype; D014764:Viral Proteins / Q000032:analysis / Q000302:isolation & purification; D014771:Virion
D014764:Viral Proteins
Apport des techniques d'immunoélectrophorèse bidimensionnelle à l'étude de la structure et de la morphogenèse de l'adénovirus humain.