Effect of lectins on the levels of cAMP and cGMP in guinea pig lymphocytes: early responses of lymph node cells to mitogenic and non-mitogenic lectins.
Guinea pig lymph node lymphocytes were reacted with two mitogenic lectins (LcL-A or PHA) and two nonmitogenic lectins (AbL or WGA)m and the levels of cAMP and cGMP were determined during 30 min of culture. Various concentrations of LcL-A and PHA caused modest (1.5 to 2-fold) rises in cAMP levels. However, the rise in cAMP did not correlate with the degree of mitogenicity. AbL caused a greater rise in cAMP than the mitogenic lectins whereas WGA had no effect on cAMP levels. None of the four lectins causes an appreciable (i.e., greater than 1.5-fold) change in cGMP levels. There does not seem to be any positive correlation between early changes of cAMP and/or cGMP and mitogenesis.
D000818:Animals; D000918:Antibody Specificity; D000242:Cyclic AMP / Q000032:analysis*; D006152:Cyclic GMP / Q000032:analysis*; D006168:Guinea Pigs; D037102:Lectins / Q000494:pharmacology*; D008198:Lymph Nodes / Q000378:metabolism*; D008214:Lymphocytes / Q000378:metabolism*; D008934:Mitogens / Q000494:pharmacology*; D013997:Time Factors
D037102:Lectins; D008934:Mitogens; D000242:Cyclic AMP; D006152:Cyclic GMP