[Research on subcellular localization of procollagen proline hydroxylase from chick embryo liver (author's transl)].
Subcellular fractionation by differential centrifugation was used to study protocollagen proline hydroxylase (EC 1.14.11.2) localization from chick embryo liver. The fractions have been characterized by marker enzymes and electron microscopy. By these methods, it was observed that procollagen-proline-hydroxylase is concentrated in the microsomal fraction which is sedimented at 145 000 X g in 250 mM sucrose.
D000818:Animals; D002458:Cell Fractionation; D002642:Chick Embryo; D003094:Collagen / Q000096:biosynthesis*; D008099:Liver / Q000201:enzymology*; D008854:Microscopy, Electron; D011393:Procollagen-Proline Dioxygenase / Q000378:metabolism*; D013347:Subcellular Fractions / Q000201:enzymology / Q000648:ultrastructure
D003094:Collagen; D011393:Procollagen-Proline Dioxygenase
Recherche de la fixation subcellulaire de la procollagène-proline-hydroxylase du foie d'embryon de poulet