title: DNA polymerase activities induced by polyoma virus infection of 3T3 mouse fibroblasts.
abstract: Resting mouse 3T3 fibroblasts were stimulated to synthesize DNA either by infection with polyoma virus or by injection of fresh serum. Changes in the levels of DNA polymerase (alpha-, beta-, and gamma-enzymes) were measured in the cytoplasm and the cell nucleus. Both types of stimulation gave very similar increases for all three enzyme activities. In the cell nucleus, both alpha- and gamma-polymerases increased almost tenfold, whereas the beta-enzyme only was stimulated twofold. In the cytoplasm alpha- and gamma-polymerases increased two- to four-fold. Only insignificant amounts of the beta-enzyme were found in the cytoplasm.
mesh_terms: D000818:Animals; D001769:Blood; D002417:Cattle; D002467:Cell Nucleus / Q000201:enzymology; D002474:Cell-Free System; D003470:Culture Media; D003593:Cytoplasm / Q000201:enzymology; D004247:DNA / Q000096:biosynthesis; D004254:DNA Nucleotidyltransferases / Q000378:metabolism*; D004279:DNA, Viral / Q000096:biosynthesis; D004790:Enzyme Induction; D005347:Fibroblasts / Q000201:enzymology*; D007527:Isoenzymes / Q000378:metabolism*; D051379:Mice; D011120:Polyomavirus / Q000254:growth & development* / Q000378:metabolism
chemical_list: D003470:Culture Media; D004279:DNA, Viral; D007527:Isoenzymes; D004247:DNA; D004254:DNA Nucleotidyltransferases