Hydrolysis in 2'-chloro-2'-deoxy nucleosides, -nucleotides and -polynucleotides to arabinose-derivatives.
The rate of hydrolysis of 2'-chloro-2'-deoxycytidine and -uridine, and their 5'-phosphates, -diphosphates and polymers to the corresponding arabinocytidine and arabinouridine derivatives in the presence of Tris-HCL pH 8.9 is determined. This rate is highest for the nucleosides, less for the 5'-phosphates and 5'-diphosphates and even less for the polynucleotides. In the the 2'-chloro-2'-deoxyuridine series the O2, 2'-cyclonucleoside was formed in addition to the arabinonucleoside.
D000469:Alkaline Phosphatase; D001089:Arabinose; D055598:Chemical Phenomena; D002621:Chemistry; D002713:Chlorine; D003841:Deoxycytidine; D003853:Deoxyribonucleosides; D003854:Deoxyribonucleotides; D006868:Hydrolysis; D007700:Kinetics; D010727:Phosphoric Diester Hydrolases; D011119:Polynucleotides; D014325:Tromethamine; D014529:Uridine
D003853:Deoxyribonucleosides; D003854:Deoxyribonucleotides; D011119:Polynucleotides; D014325:Tromethamine; D003841:Deoxycytidine; D002713:Chlorine; D001089:Arabinose; D000469:Alkaline Phosphatase; D010727:Phosphoric Diester Hydrolases; D014529:Uridine