[Suitability of immune serums from different animal species for the preparation of conjugates against Aujeszky's disease virus].
A comparison was made of immune serum from rabbits, sheeps, pigs and cattle. Pure IgG was prepared by the Rivanol sulphate method and immune electrophoresis. Optimum protein quality, conjugation time under various conditions and using two preparations of fluorescein isothiocyanate were studied. Conjugate purified by DEAE-Sephadex A-50 and concentrated to 1% protein by ultrafiltration was freeze-dried and then tested for activity and the degree of non-specific fluorescence. Evaluation by yield of immuno-electrophoretically pure IgG, conjugation time, antibody content, degree of non-specific fluorescence and the intensity of specific fluorescence before and after dilution showed that pig serum was the best, followed by cattle and sheep serum; rabbit serum was the least suitable for conjugate preparation.
D000818:Animals; D002417:Cattle / Q000276:immunology; D006564:Herpesviridae / Q000276:immunology*; D011558:Herpesvirus 1, Suid / Q000276:immunology*; D007122:Immunoelectrophoresis / Q000662:veterinary; D007074:Immunoglobulin G / Q000032:analysis; D011817:Rabbits / Q000276:immunology; D012756:Sheep / Q000276:immunology; D013552:Swine / Q000276:immunology; D013678:Technology, Pharmaceutical; D014765:Viral Vaccines
D007074:Immunoglobulin G; D014765:Viral Vaccines
Studium der Eignung von Immunseren mehrerer Tierarten zur Herstellung eines Konjugates gegen das Virus der Aujeszkyschen Krankheit