A new histochemical method using human placenta alkaline phosphatase for demonstrating concanavalin A binding sites on cell surfaces.
Human placenta alkaline phosphatase (HP-ALP), a glycoprotein, was stained histochemically for the purpose of examining the concanavalin A (Con A) binding sites on the cell surface. HP-ALP was bound to the cell surface by Con A. This simple method successfully detected Con A binding sites on the cell surface.
D000469:Alkaline Phosphatase; D000818:Animals; D001665:Binding Sites; D001717:Birds; D003208:Concanavalin A / Q000378:metabolism*; D005260:Female; D006651:Histocytochemistry / Q000379:methods*; D006801:Humans; D051379:Mice; D010920:Placenta / Q000201:enzymology; D011247:Pregnancy; D001357:Sarcoma, Avian / Q000378:metabolism*
D003208:Concanavalin A; D000469:Alkaline Phosphatase