Mutation induction by 5-fluorodeoxyuridine in synchronous Chinese hamster cells.
Chinese hamster ovary cells synchronized by mitotic detachment were treated with 5-fluorodeoxyuridine (FdUrd), 0.2 micrograms/ml, at various times in the cell cycle. FdUrd treatment in the early S period induced resistance to 6-thioguanine. Treatment of asynchronous cells with FdUrd caused little increase in 6-thioguanine resistance over the spontaneous frequency. Mitotic selection thus enhances the probability of a cell being in the portion of S period receptive to mutation. These observations suggest that FdUrd treatment leads to mutagenesis at the growing points of DNA replication.
D000818:Animals; D002453:Cell Cycle; D002460:Cell Line; D006224:Cricetinae; D004351:Drug Resistance; D005260:Female; D005467:Floxuridine / Q000494:pharmacology*; D009153:Mutagens; D009154:Mutation / Q000187:drug effects*; D010053:Ovary; D013866:Thioguanine / Q000494:pharmacology; D013936:Thymidine / Q000494:pharmacology
D009153:Mutagens; D005467:Floxuridine; D013866:Thioguanine; D013936:Thymidine