An improved method for purifying sialidase.
An adsorbent specific for sialidase (EC 3.2.1.18) was prepared by coupling a glycoprotein containing glycosidically linked sialic acid to Sepharose. This adsorbent does not display the non-specific adsorption that occurs in previously reported methods.
D000327:Adsorption; D003016:Clostridium perfringens / Q000201:enzymology; D006023:Glycoproteins; D006388:Hemagglutinins / Q000032:analysis; D006460:Hemolysin Proteins / Q000032:analysis; D009439:Neuraminidase / Q000302:isolation & purification*; D009961:Orosomucoid; D012685:Sepharose
D006023:Glycoproteins; D006388:Hemagglutinins; D006460:Hemolysin Proteins; D009961:Orosomucoid; D012685:Sepharose; D009439:Neuraminidase