Nuclear protein kinase of brain: effect of S-100 protein.
The non-histone chromosomal protein fraction isolated from purified brain nuclei possesses protein kinase activity. 93% of this activity is lost by heating at 80 degrees C for 5 min. cAMP does not affect the reaction, but cGMP is inhibitory. In the presence of S-100, an acidic brain-specific protein, phosphate incorporation is enhanced 3 to 4 fold. Bovine serum albumin has no effect whereas histone inhibits activity.
D000818:Animals; D001921:Brain / Q000201:enzymology* / Q000648:ultrastructure; D002467:Cell Nucleus / Q000201:enzymology; D002875:Chromosomes / Q000378:metabolism; D000242:Cyclic AMP / Q000494:pharmacology; D006152:Cyclic GMP / Q000494:pharmacology; D006657:Histones / Q000494:pharmacology; D006358:Hot Temperature; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D009419:Nerve Tissue Proteins / Q000494:pharmacology*; D011494:Protein Kinases / Q000378:metabolism*; D051381:Rats; D009418:S100 Proteins / Q000494:pharmacology*; D012710:Serum Albumin, Bovine / Q000494:pharmacology; D014158:Transcription, Genetic
D006657:Histones; D009419:Nerve Tissue Proteins; D009418:S100 Proteins; D012710:Serum Albumin, Bovine; D000242:Cyclic AMP; D011494:Protein Kinases; D006152:Cyclic GMP