Localization of deoxyribonucleic acid-stimulated adenosine triphosphatases in human lymphocytes.
Two enzymes hydrolyzing ATP (ATPase A and ATPase B) were purified from freshly isolated lymphocytes of human tonsils. Both enzymes are stimulated by single-stranded DNA and seem to be localized in the chromatin. ATPase A and ATPase B appear to be distinct enzymes as judged from their elution profiles obtained after DEAE-cellulose and ATP-Sepharose column chromatography, from their behavior towards actinomycin D, a DNA intercalating agent, and from their sensitivity to monovalent salt concentration.
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D002413:Cations, Divalent; D002843:Chromatin / Q000201:enzymology; D004277:DNA, Single-Stranded / Q000494:pharmacology*; D004789:Enzyme Activation; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism*; D007700:Kinetics; D008214:Lymphocytes / Q000201:enzymology*; D014066:Palatine Tonsil / Q000201:enzymology
D002413:Cations, Divalent; D002843:Chromatin; D004277:DNA, Single-Stranded; D007527:Isoenzymes; D000251:Adenosine Triphosphatases