Studies on the terminal stages of antibody-complement-mediated killing of a tumor cell. I. Evidence for the existence of an intermediate, T.
The mechanism of the terminal steps in the lysis of antibody-sensitized tumor cells by complement (TAC) was studied. It was shown that once complement has reacted, lysis proceeded even in the absence of fluid-phase complement. Transformation of TAC to dead cells was found to be at least a two-step process: one of the steps was temperature dependent whereas the other was reversibly inhibited by EDTA. In analogy to the hemolytic system, TAC has been designated T.
D000818:Animals; D000906:Antibodies; D006528:Carcinoma, Hepatocellular / Q000139:chemically induced; D002460:Cell Line; D003165:Complement System Proteins / Q000378:metabolism*; D003601:Cytotoxicity Tests, Immunologic; D004052:Diethylnitrosamine; D004492:Edetic Acid / Q000494:pharmacology; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008113:Liver Neoplasms; D013696:Temperature; D013997:Time Factors
D000906:Antibodies; D004052:Diethylnitrosamine; D003165:Complement System Proteins; D004492:Edetic Acid