Regulation of tumor antigen synthesis by simain virus 40 gene A.
Simian virus 40 gene A has previously been shown to promote the replication of viral DNA and the transcription of late viral RNA in productive infection and to maintain the growth characteristics of some transformed cells. The present study examines the effect of the A function on proteins synthesized during productive and transforming infections. Under restrictive conditions, temperature-sensitive A mutants induce the overproduction of a 100,000-dalton protein both in productively infected monkey cells and in transformed rabbit cells. Immunoprecipitation of the induced protein with antisera, prepared against simian virus 40-induced tumors in hamsters, was used to identify the induced protein as tumor antigen. The same protein can be precipitated from extracts of cells infected by wild-type virus but not from uninfected cells. Furthermore, the mutant-induced protein is more rapidly degraded in vivo and is less tightly bound to intranuclear components than the protein induced by wild-type virus. The presence of the same virus-induced protein in infected cells from different species and the altered behavior of that protein in mutant infection strongly suggest that the protein is virus coded. Because the protein is large enough to account for the entire coding capacity in the early region of the simian virus 40 genome, the 100,000-dalton protein may well be the primary product of the only early gene identified by complementation studies, the A gene. If the 100,000-dalton protein that is overproduced in mutant infection is the A protein and the only early protein, then functional wild-type A protein must regulate its own synthesis in both productive and transforming infections.
D000818:Animals; D000936:Antigen-Antibody Complex; D000951:Antigens, Neoplasm; D001345:Autoradiography; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D011232:Chemical Precipitation; D002999:Clone Cells; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D005796:Genes; D000882:Haplorhini; D007668:Kidney; D008970:Molecular Weight; D009154:Mutation; D011817:Rabbits; D013539:Simian virus 40 / Q000276:immunology / Q000378:metabolism*; D013696:Temperature; D014764:Viral Proteins / Q000096:biosynthesis* / Q000276:immunology
D000936:Antigen-Antibody Complex; D000951:Antigens, Neoplasm; D014764:Viral Proteins