Primary tissue culture of normal adult human decapsulated adrenal cortex: radioautographic studies on the metabolic effects of ACTH1-24.
ACTH-deprived primary normal adult human adrenocortical cells on the 23rd day in vitro were found to lack any DNA synthetic and proliferative activity, but incorporated uridine-3H and 1-leucine-3H actively. In cortical cells of the same age treated for 2 and 7 days with ACTH1-24 (3 X 10(-6) M) the incorporation of the precursors for RNA and DNA synthesis and the cell proliferation were found to be markedly stimulated. The apparent uptake of leucine-3H was instead reduced in 2-day and, less still, in 7-day treated cells. In parallel with ultrastructural studies, these data suggest that ACTH orderly activates the template activity of chromatin while eliciting the differentiation and hypertrophy of human adrenocortical cells.
D000302:Adrenal Cortex / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D000311:Adrenal Glands / Q000378:metabolism*; D000324:Adrenocorticotropic Hormone / Q000031:analogs & derivatives*; D000328:Adult; D001345:Autoradiography; D002466:Cell Nucleolus / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D003366:Cosyntropin / Q000494:pharmacology*; D003593:Cytoplasm / Q000378:metabolism; D004261:DNA Replication; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D013936:Thymidine / Q000378:metabolism; D014529:Uridine / Q000378:metabolism
D003366:Cosyntropin; D000324:Adrenocorticotropic Hormone; D013936:Thymidine; D014529:Uridine