title: [Synthesis and properties of 5-acetyl-4-methyl-1-(beta-d-ribofuranosyl)-imidazole-5' di-and-triphosphate].
abstract: 5-Acetyl-4-methyl-1-(beta-D-ribofuranosyl)-imidazole-5'-phosphate reacts with diphenylphospho chloridate forming the asymmetrical pyrophosphate ester. This in turn reacts with tri-n-butyl-ammonium phosphate yielding 5-acetyl-4-methyl-imidazole-riboside-5'-diphosphate and with tri-n-butylammonium pyrophosphate to give the nucleotide triphosphate. 5-Acetyl-4-methyl-imidazole-riboside-5'-pyrophosphate shows in the test with pyruvate kinase a reaction rate three times slower than that of ADP; but the same Km as that of ADP. The ATP analogue is only about 10% as effective as ATP itself in the test with hexokinase, 3-phosphoglycerate kinase and gloconate kinase. Adenylate kinase and NAD" kinase show no activity when ATP is replaced by the nucleotide-triphosphate-analogue. In presence of ATP the analogue strongly inhibits the reaction of adenylate kinase.
mesh_terms: D000085:Acetates / Q000032:analysis / Q000138:chemical synthesis*; D000244:Adenosine Diphosphate / Q000138:chemical synthesis; D000255:Adenosine Triphosphate / Q000138:chemical synthesis; D002845:Chromatography; D007093:Imidazoles / Q000032:analysis / Q000138:chemical synthesis*; D009243:NAD; D012343:RNA, Transfer; D012266:Ribose / Q000032:analysis / Q000138:chemical synthesis*; D013403:Sugar Phosphates / Q000032:analysis / Q000138:chemical synthesis*
chemical_list: D000085:Acetates; D007093:Imidazoles; D013403:Sugar Phosphates; D009243:NAD; D000244:Adenosine Diphosphate; D012266:Ribose; D000255:Adenosine Triphosphate; D012343:RNA, Transfer
vernacular_title: Darstellung und Eigenschaften von 5-Acetyl-4-methyl-1-(beta-D-ribofuranosyl)-imidazol-5'-di- und -triphosphat