Determination of human liver cytochrome P-450 by electron paramagnetic resonance of liver biopsies.
Human liver cytochrome P-450 level has beenen determined by direct electron paramagnetic resonance spectroscopy at 77 degrees K of liver specimens obtained by surgical biopsy. A preliminary experiment with rat livers was performed in order to calibrate the intensity of the cytochrome P-450 EPR signal as a function of the cytochrome concentration obtained by the classical spectrophotometric method. 44 subjects have be examined. We found a cytochrome P-450 value of 21.5 plus or minus 11.2 nmoles per gram of liver in normal subjects. Variations of this level in some pathological liver states are discussed.
D000818:Animals; D001706:Biopsy; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis*; D004578:Electron Spin Resonance Spectroscopy; D006506:Hepatitis A / Q000201:enzymology; D006801:Humans; D008099:Liver / Q000187:drug effects / Q000201:enzymology* / Q000473:pathology; D008103:Liver Cirrhosis / Q000201:enzymology; D008107:Liver Diseases / Q000201:enzymology; D009574:Nitro Compounds / Q000009:adverse effects; D051381:Rats; D013045:Species Specificity; D013844:Thiazoles / Q000009:adverse effects
D009574:Nitro Compounds; D013844:Thiazoles; D003577:Cytochrome P-450 Enzyme System