[Differences in the enzymatic activity of mitochondria from enriched neuronal and glial fractions].
Studies were performed in the activities of certain enzymes from oxidoreductase group: cytochrome c-oxidoreductase (EC 1.6.99.3), succinate dehydrogenase succinates: cytochrome c-oxidoreductase (EC 1.3.99.1), cytochrome oxidase (EC 1.9.3.1) and malate dehydrogenase (EC 1.1.1.37) in mitochondria from neuronal and glial-enriched fractions. The mitochondrial fraction purity was observed by the electron microscope. The enzyme activity of the glial mitochondrial fraction was much higher than that in the neuronal mitochondria. Malate dehydrogenase from glial enriched fraction consists of three isoenzymes, while neuronal mitochondria had two isoenzymes of malate dehydrogenase. The neuronal mitochondria were found to be more stable to lubrol and digitonin.
D000818:Animals; D001921:Brain / Q000648:ultrastructure*; D003579:Cytochrome Reductases / Q000378:metabolism; D004072:Digitonin / Q000494:pharmacology; D003576:Electron Transport Complex IV / Q000378:metabolism; D004789:Enzyme Activation; D005029:Ethylenediamines / Q000494:pharmacology; D005260:Female; D007527:Isoenzymes / Q000378:metabolism; D008291:Malate Dehydrogenase / Q000378:metabolism; D008854:Microscopy, Electron; D008928:Mitochondria / Q000187:drug effects / Q000201:enzymology*; D009457:Neuroglia / Q000201:enzymology*; D009474:Neurons / Q000201:enzymology*; D051381:Rats; D013229:Stearic Acids / Q000494:pharmacology; D013384:Succinate Cytochrome c Oxidoreductase / Q000378:metabolism; D013385:Succinate Dehydrogenase / Q000378:metabolism
D005029:Ethylenediamines; D007527:Isoenzymes; D013229:Stearic Acids; D013384:Succinate Cytochrome c Oxidoreductase; D008291:Malate Dehydrogenase; D013385:Succinate Dehydrogenase; D003579:Cytochrome Reductases; D003576:Electron Transport Complex IV; D004072:Digitonin
Razlichiia v aktivnosti fermentov mitokhondriĭ iz obogashchennykh neĭronal'noĭ i glial'noĭfraktsiĭ