[Temperature dependence of the epr spectra of a spin-labeled myosin and its complex with adp].
The effect of temperature on ESR spectra of spin-labelled myosin and myosin-ADP complex was studied. It was shown that mobility of the sptrongly immobilized spin label almost did not change with temperature (-2 degrees C-37 degrees C) whereas mobility of the slightly immobilized spin label changed in a complicated manner and not monotonously. The observed changes in mobility of the slightly immobilized spin label and characteristics of the ESP spectrum of the myosin-ADP complex suggest that temperature causes continuous changes of the structure in the local environment of the binding sites of the spin label, and on the average, one conformation of myosin and myosin-ADP complex molecules exists at any temperature.
D000244:Adenosine Diphosphate; D000818:Animals; D001665:Binding Sites; D003497:Cyclic N-Oxides; D004578:Electron Spin Resonance Spectroscopy; D005292:Ferricyanides; D009218:Myosins; D011817:Rabbits; D013113:Spin Labels; D013696:Temperature
D003497:Cyclic N-Oxides; D005292:Ferricyanides; D013113:Spin Labels; D000244:Adenosine Diphosphate; D009218:Myosins
Temperaturnaia zavisimost' spektrov EPR spin-mechenogo miozina i ego kompleksa s ADF