title: Isolation and properties of reovirus from cattle in an outbreak of acute respiratory disease.
abstract: A cytopathogenic virus was isolated in the primary culture of bovine kidney cells from a nasal swab of affected calves in an outbreak of acute respiratory disease in Japan in 1971. It agglutinated human type O erythrocytes and produced cytoplasmic inclusion bodies. Viral replication was inhibited by 5-iodo-2'-deoxyuridine, indicating that the viral nucleic acid was RNA. The virus was resistant to ether, chloroform, sodium deoxycholate, and acid, and passed readily through Sartorius' membrane filter 100 nm in pore size, but not through the filter 50 nm in pore size. Electron microscopy showed many spherical particles 60 approximately 75 nm in diameter with a double-layered capsid in a sample taken at a buoyant density of 1.34 produced by CaCl equilibrium centrifugation. The virus suspended in 1M MgCl2 solution was stable against heating at 50 degrees C for 30 minutes, but not against freezing at -20 degrees C for 60 minutes. The virus was resistant to, and increased in infectivity after, treatment with 0.063 approximately 1.0% trypsin. These properties were consistent with those established for the reoviruses. Most affected cattle showed a significant rise of antibody titer against reovirus and bovine respiratory syncytial virus, whereas only a few of them presented a serological evidence for recent infection with parainfluenza virus type 3, bovine adenovirus type 7, and bovine parovirus.
mesh_terms: D000208:Acute Disease; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D002712:Chlorides / Q000494:pharmacology; D003588:Cytopathogenic Effect, Viral; D003840:Deoxycholic Acid / Q000494:pharmacology; D004196:Disease Outbreaks / Q000662:veterinary*; D008274:Magnesium / Q000494:pharmacology; D009696:Nucleic Acids / Q000302:isolation & purification; D012087:Reoviridae / Q000254:growth & development / Q000276:immunology / Q000302:isolation & purification*; D012088:Reoviridae Infections / Q000382:microbiology / Q000662:veterinary*; D012141:Respiratory Tract Infections / Q000382:microbiology / Q000662:veterinary*; D014357:Trypsin / Q000494:pharmacology
chemical_list: D002712:Chlorides; D009696:Nucleic Acids; D003840:Deoxycholic Acid; D014357:Trypsin; D008274:Magnesium