Isolation of Wilms' tumor antigens by chelation.
A procedure for ethylenediaminetetraacetate extraction of minced Wilm's tumor was assessed as a method for isolating Wilm's tumor antigens. An antigen was detected by immunodiffusion using an adsorbed antiserum to this extract. This antigen was also found in ethylenediaminetetraacetate extracts of in vitro cultures of nephroblastoma cells.
D000818:Animals; D000951:Antigens, Neoplasm / Q000302:isolation & purification*; D002478:Cells, Cultured; D004492:Edetic Acid; D006801:Humans; D005779:Immunodiffusion / Q000379:methods; D011817:Rabbits / Q000276:immunology; D009396:Wilms Tumor / Q000276:immunology*
D000951:Antigens, Neoplasm; D004492:Edetic Acid