Presence of the methylester of 5-carboxymethyl uridine in the wobble position of the anticodon of tRNAIII Arg from brewer's yeast.
The methylester of 5-carboxymethyluridine (mcm5U), its degradation product 5-carboxymethyluridine (cm5U) and the corresponding nucleotide (cm5Up) were isolated from brewer's yeast tRNAIII Arg or from the dodecanucleotide containing the anticodon. Their chromatographic and electrophoretic properties and their UV absorbing spectra were identical to that of the corresponding synthetic compounds. The gas chromatographic behavior and the mass spectrum of mcm5U obtained from tRNAIII Arg and of a synthetic sample were also identical ; the rare occurence of a thermal reciprocal bimolecular methyl-hydrogen transfer in the mass spectrometer ion source was observed. A mild alkaline treatment of tRNAIII Arg leads to the saponification of mcm5U into cm5U (within the tRNA), which can be again esterified in the presence of a yeast homogenate and (methyl-14C) S adenosylmethionine. The radioactivity was found in the mcm5U located in the wobble position of the anticodon of tRNAIII Arg. The presence of this odd nucleotide in that position could possibly restrict the codon-anticodon interaction of tRNAIII Arg.
D000926:Anticodon; D001120:Arginine; D002250:Carbon Radioisotopes; D002264:Carboxylic Acids; D002848:Chromatography, DEAE-Cellulose; D002849:Chromatography, Gas; D002854:Chromatography, Paper; D002855:Chromatography, Thin Layer; D004590:Electrophoresis, Paper; D004926:Escherichia coli / Q000201:enzymology; D013058:Mass Spectrometry; D008745:Methylation; D010179:Pancreas / Q000201:enzymology; D010744:Phosphoric Monoester Hydrolases; D012343:RNA, Transfer; D012260:Ribonucleases; D013056:Spectrophotometry, Ultraviolet; D014529:Uridine / Q000031:analogs & derivatives / Q000032:analysis; D015003:Yeasts / Q000032:analysis*
D000926:Anticodon; D002250:Carbon Radioisotopes; D002264:Carboxylic Acids; D012343:RNA, Transfer; D001120:Arginine; D012260:Ribonucleases; D010744:Phosphoric Monoester Hydrolases; D014529:Uridine