Herpes simplex-RAJI(A44), a new cell line for serologic testing by immunofluorescence.
An IF technique for the detection of HSV antibodies by using a lymphoblastoid cell line, Raji(A44), which continuously produce a constant amount of HSV antigen, is described. IF and IH tests were found to be similar with respect to sensitivity and reproducibility. Sero-conversions detected by the CF test were detected with this cell line. The Raji(A44) line (cells in suspension) can be routinely used for the diagnosis of HSV infection.
D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D004854:Herpesvirus 4, Human / Q000276:immunology; D008722:Methods; D018139:Simplexvirus / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral