title: The mutability of the minute loci of Drosophila melanogaster with ethyl methanesulfonate.
abstract: It has been suggested that the Minute loci of Drosophila melanogaster are the redundant structural loci for the transfer RNA's [31]. To inquire whether the Minute loci differed from other loci in their genetic organization we have determined the dose response curves for the induction of Minutes and sex-linked recessive lethals with ethyl methanesulfonate (EMS). There are approx. 67.75 +/- 9.35 Minute mutants induced for every 5000 recessive lethals induced in the genome and this relationship is independent of EMS dosage. This is in good agreement with the relative numbers of Minute and lethal loci in the genome. Because the target size of the average Minute locus is the same as that of the average locus capable of mutating to a lethal, these data do not support the view that the Minute loci are special in their genetic organization. Since Minute mutants can be scored in the F1 of mutagenized flies it is suggested that the induction of Minute mutants may provide a more rapid and economical means of assessing mutagenicity than do traditional screens for the induction of recessive lethals.
mesh_terms: D000818:Animals; D002875:Chromosomes; D004305:Dose-Response Relationship, Drug; D004331:Drosophila melanogaster; D005020:Ethyl Methanesulfonate / Q000633:toxicity*; D005260:Female; D005796:Genes / Q000187:drug effects*; D005804:Genes, Lethal / Q000187:drug effects; D005808:Genes, Recessive; D008297:Male; D008698:Mesylates / Q000633:toxicity*; D009030:Mosaicism; D009154:Mutation
chemical_list: D008698:Mesylates; D005020:Ethyl Methanesulfonate