Fluorescence of tryptophan-containing peptides on paper or silica gel after treatment with formaldehyde, formaldehyde-ozone or formaldehyde-hydrochloric acid.
Sensitive and specific procedures for the chromatographic detection of tryptophan and tryptophan-containing peptides are described. Formaldehyde gas induces strong and characteristic fluorescence from tryptophan and peptides with NH2-terminal tryptophan residues on silica gel. On filter-paper, the detection of small amounts of these compounds requires the additional use of an oxidant, such as ozone. Treatment with formaldehyde-hydrochloric acid was used as a method for inducing fluorescence from tryptophan-containing peptides regardless of the position of the tryptophan residue in the peptide molecule. This reaction is useful for the chromatographic demonstration of small amounts of such peptides on both paper and silica gel. The spectral properties of the fluorophores of such tryptophan-containing peptides are distinctive and serve to distinguish them from all other known biogenic compounds that are capable of giving fluorescence with formaldehyde.
D002850:Chromatography, Gel; D002854:Chromatography, Paper; D005557:Formaldehyde; D006851:Hydrochloric Acid; D007202:Indicators and Reagents; D008722:Methods; D010126:Ozone; D010455:Peptides / Q000032:analysis*; D012822:Silicon Dioxide; D013050:Spectrometry, Fluorescence; D014364:Tryptophan / Q000032:analysis*
D007202:Indicators and Reagents; D010455:Peptides; D005557:Formaldehyde; D010126:Ozone; D012822:Silicon Dioxide; D014364:Tryptophan; D006851:Hydrochloric Acid