Thf complete amino acid sequence of C-I (apoLp-Ser), an apolipoprotein from human very low density lipoproteins.
C-I was prepared from very low density lipoproteins of patients with familial type V hyperliporproteinemia. Peptides from tryptic digests of unmodified and succinylated C-I, chymotryptic peptides, and the products of cayanogen bromide cleavage were isolated and characterized. Sequence analysis of tryptic peptides was performed by the dansyl (5-dimethylaminonaphthalene-1-sulfonyl) technique and hydrolytic regeneration  of the amino acid residues from the phenylthiocarbamyl derivatives. Alignment of the tryptic fragments within the cyanogen bromide and succinyl-tryptic peptides was confirmed by the overlap chymotryptic peptides. The complete amino acid sequence of C-I, 57 residues in length, does not reveal any obvious basis for its lipophilic properties.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000626:Aminopeptidases; D001059:Apoproteins / Q000097:blood; D002268:Carboxypeptidases; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D002855:Chromatography, Thin Layer; D002918:Chymotrypsin; D003488:Cyanogen Bromide; D003619:Dansyl Compounds; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood / Q000235:genetics; D008079:Lipoproteins, VLDL / Q000097:blood; D010446:Peptide Fragments / Q000032:analysis; D010447:Peptide Hydrolases; D012694:Serine; D014357:Trypsin
D000596:Amino Acids; D001059:Apoproteins; D003619:Dansyl Compounds; D008079:Lipoproteins, VLDL; D010446:Peptide Fragments; D012694:Serine; D002268:Carboxypeptidases; D010447:Peptide Hydrolases; D000626:Aminopeptidases; D002918:Chymotrypsin; D014357:Trypsin; D003488:Cyanogen Bromide