An immunoprecipitin study of the incidence of influenza A antibodies in animal sera in the Ottawa area.
A survey of over 600 'normal' sera from 14 animal species by immunoprecipitin tests in cellulose acetate using viron antigens revealed a high incidence of precipitating activity against a broad range of influenza A virus strains, particularly A2hHong Kong/1/68 and /PR8. However, serum treatments trypsin-heat-periodate, NaIO4, V. cholerae receptor-destroying enzyme (RDE), or kaolin eliminated most precipitating activity, which suggests that it was due to "non-specific" inhibitors of influenze viruses. A resistant minority could not be identified as inhibitor or antibody on this basis. Precipitation of the influenza A major type-specific antigen in virus-soluble antigens by human 7S gamma globulin antibody (IgG), demonstrated to be specific for influenza virus, was established as a reference reaction to identify similar immunoprecipitin reactions occurring between virus-soluble antigens and normal or immune sera. Complement fixation tests provided supplementary evidence for the presence of influenza A antibodies in these sera. Influenza A antibodies were found in only a few sera of six animal species: cat, dog, rabbit, goat, chipmunk, and sheep. Thus the animal species examined in the Ottawa area have not revealed an unequivocal reservoir for human influenza A viruses.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000956:Antigens, Viral; D000998:Antiviral Agents; D001717:Birds; D002170:Canada; D002415:Cats; D002417:Cattle; D003168:Complement Fixation Tests; D003289:Convalescence; D004285:Dogs; D006041:Goats; D006385:Hemagglutination Inhibition Tests; D006736:Horses; D006801:Humans; D005779:Immunodiffusion; D007074:Immunoglobulin G / Q000032:analysis; D009980:Influenza A virus / Q000276:immunology*; D007251:Influenza, Human / Q000276:immunology; D008322:Mammals; D009975:Orthomyxoviridae / Q000276:immunology*; D010222:Parainfluenza Virus 1, Human / Q000276:immunology; D011817:Rabbits; D012756:Sheep; D013045:Species Specificity
D000906:Antibodies; D000956:Antigens, Viral; D000998:Antiviral Agents; D007074:Immunoglobulin G