Incorporation of 75Se-selenomethionine into human apoproteins. I. Characterization of specificity in very-low-density and low-density lipoproteins.
Characterization of specificity of incorporation of 75Se-selenomethionine into human apoproteins following in-vivo injection of the radioactive amino acid precursor is reported. Apoprotein fractionation was attained by serial ultracentrifugation, anhydrous partial delipidization, decyl sulfate solubilization with Sephadex G-150 gel filtration into polypeptide fractions, Sf-I, and Sf-II, for VLDL, and LDL subfractions. Similarity of the Sf-I fractions isolated from VLDL, LDL1, and LDL2 was defined by gel exclusion volume, disk-gel electrophoresis, immunodiffusion, and amino acid composition. Enrichment of the Sf-I fractions with 75Se-SM in VLDL and LDL subfractions was observed, suggesting a possible use for this isotopic method for the investigation of apoprotein metabolism.
D000596:Amino Acids / Q000378:metabolism; D001059:Apoproteins / Q000097:blood / Q000302:isolation & purification; D002784:Cholesterol / Q000378:metabolism; D006801:Humans; D008074:Lipoproteins / Q000302:isolation & purification; D008077:Lipoproteins, LDL / Q000097:blood*; D008079:Lipoproteins, VLDL / Q000097:blood*; D010446:Peptide Fragments / Q000378:metabolism; D012643:Selenium / Q000378:metabolism*; D012645:Selenomethionine / Q000378:metabolism*; D014280:Triglycerides / Q000378:metabolism
D000596:Amino Acids; D001059:Apoproteins; D008074:Lipoproteins; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D010446:Peptide Fragments; D014280:Triglycerides; D012645:Selenomethionine; D002784:Cholesterol; D012643:Selenium