Phosphodiesterase and adenyl-cyclase activities in the cerebral cotex of the aging rat.
In an effort to determine the factors responsible for the four-fold decrease in cyclic-AMP content of the rat cerebral cortex, observed to occur between the ages of 3 and 6 months, studies were performed on adenyl-cyclase and phosphodiesterase, the cyclic-AMP synthesizing and hydrolyzing enzymes. The activities and kinetic characteristics were determined for both enzymes as obtained from the cerebral cortex of rats ranging in age from one to 24 months. No age dependence was observed either in adenyl-cyclase activity, assayed with or without fluoride ion, or in phosphodiesterase activity. It was concluded that age related changes in factors other than the direc levels of these enzymes underlie the age related decline in cortical cyclic-AMP levels.
D000249:Adenosine Monophosphate / Q000134:cerebrospinal fluid / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000134:cerebrospinal fluid / Q000378:metabolism; D000262:Adenylyl Cyclases / Q000134:cerebrospinal fluid / Q000378:metabolism*; D000375:Aging; D000818:Animals; D002540:Cerebral Cortex / Q000201:enzymology*; D004796:Clinical Enzyme Tests / Q000379:methods; D004789:Enzyme Activation / Q000187:drug effects; D005459:Fluorides / Q000494:pharmacology; D008297:Male; D010727:Phosphoric Diester Hydrolases / Q000134:cerebrospinal fluid / Q000378:metabolism*; D051381:Rats
D000249:Adenosine Monophosphate; D000255:Adenosine Triphosphate; D010727:Phosphoric Diester Hydrolases; D000262:Adenylyl Cyclases; D005459:Fluorides