[Corticosterone, a synchronizing factor for liver cells].
The clone Fu 5 a of the Reuber Hepatoma cell line was incubated with corticosterone for 48 hours. The incorporation of labelled thymidine into DNA was inhibited to 10%. After this time corticosterone was removed from the medium. The DNA-synthesis increased for ten hours, the maximum was sixteen times higher than in the controls. The conclusion drawn was that corticosterone is able to block liver cells in a part of G1 phase of the cell cycle, so that the cells become synchronous.
D006528:Carcinoma, Hepatocellular / Q000473:pathology; D002455:Cell Division / Q000187:drug effects*; D003345:Corticosterone / Q000494:pharmacology*; D046508:Culture Techniques; D004247:DNA / Q000096:biosynthesis; D006801:Humans; D008099:Liver / Q000187:drug effects*; D008113:Liver Neoplasms; D013936:Thymidine / Q000378:metabolism
D004247:DNA; D013936:Thymidine; D003345:Corticosterone
Corticosteron, ein Synchronisator für Leberzellen