[Use of the concanavalin-peroxidase-DAB system for identification of human chromosomes].
The effect of concanavalin A on the prometaphase chromosomes was investigated, using a staining reaction based on the peroxydase-diaminobenzidin-H2O2 system. After incubation with concanavalina A, the chromosomes telomeres as well as the centromeres and satellites of the acrocentric chromosomes were strongly stained. Sometimes the chromatids appeared to be coiled. In other respect, it must be noted that peroxydase alone can stain the chromatids, which probably means that this compounds is able to unite with the chromosomes, without the aid of concanavalin A.
D015100:3,3'-Diaminobenzidine; D002877:Chromosomes, Human / Q000648:ultrastructure*; D003208:Concanavalin A; D006801:Humans; D007621:Karyotyping; D008214:Lymphocytes / Q000166:cytology; D008677:Metaphase; D010544:Peroxidases
D003208:Concanavalin A; D015100:3,3'-Diaminobenzidine; D010544:Peroxidases
Utilisation du système concanavaline-peroxydase-DAB pour l'identification des chromosomes humains.