Long-term preservation of transfecting activity of DNA isolated from rat virogenic XC cells transformed by Prague strain of Rous sarcoma virus.
The DNA isolated from rat virogenic XC cells transformed by Rous sarcoma virus retains standard transfecting activity for 7 months when the DNA solution in 0.1 X PBS, containing 10% glycerol is stored at -70 degrees C. The value of the sedimentation constant S20w does not significantly change during storage.
D000818:Animals; D001358:Avian Sarcoma Viruses; D001435:Bacteriophages; D002460:Cell Line; D004273:DNA, Neoplasm / Q000302:isolation & purification* / Q000378:metabolism; D004279:DNA, Viral; D051381:Rats; D001357:Sarcoma, Avian / Q000378:metabolism*; D013997:Time Factors; D014170:Transformation, Genetic
D004273:DNA, Neoplasm; D004279:DNA, Viral