The mechanism of DNA strand breakage by vitamin C and superoxide and the protective roles of catalase and superoxide dismutase.
Vitamin C breaks DNA only in the presence of oxygen. Superoxide dismutase has no effect on the reaction but catalase suppresses it. Superoxide also gives rise to breaks in DNA suppressible by both superoxide dismutase and catalase. The hydroxyl radical seems to be the agent responsible for strand cleavage itself.
D001205:Ascorbic Acid; D001565:Benzoates / Q000494:pharmacology; D001665:Binding Sites; D002374:Catalase / Q000378:metabolism*; D004247:DNA; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D000431:Ethanol / Q000494:pharmacology; D005561:Formates / Q000494:pharmacology; D005609:Free Radicals; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007454:Iodides / Q000494:pharmacology; D007700:Kinetics; D008353:Mannitol / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000378:metabolism*; D013481:Superoxides
D001565:Benzoates; D005561:Formates; D005609:Free Radicals; D007454:Iodides; D013481:Superoxides; D000431:Ethanol; D008353:Mannitol; D004247:DNA; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase; D001205:Ascorbic Acid; D004121:Dimethyl Sulfoxide