Assembly of complex III into newly developing mitochondrial membranes.
Yeast cells grown anaerobically in 0.02% linoleic acid were transferred to air in the presence of 0.02% elaidic acid. At varying times Arrhenius plots were made of QH2-cytochrome c reductase activities in isolated mitochondria. A transition temperature of 8.2 degrees C at 0.5 h was characteristic of linoleate; at 3 h the transition temperature was increased to 24 degrees C characteristic of elaidate. At early times the enzyme was associated with anaerobic promitochondrial membranes; at later states the newly synthesized enzyme was associated with newly developed elaidate membranes.
D000332:Aerobiosis; D000693:Anaerobiosis; D003579:Cytochrome Reductases / Q000378:metabolism*; D003574:Cytochrome c Group; D005231:Fatty Acids, Unsaturated / Q000378:metabolism; D007700:Kinetics; D008041:Linoleic Acids / Q000378:metabolism; D008566:Membranes / Q000201:enzymology; D008928:Mitochondria / Q000378:metabolism*; D009097:Multienzyme Complexes / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013696:Temperature; D013816:Thermodynamics; D014451:Ubiquinone
D003574:Cytochrome c Group; D005231:Fatty Acids, Unsaturated; D008041:Linoleic Acids; D009097:Multienzyme Complexes; D014451:Ubiquinone; D003579:Cytochrome Reductases