Repair of DNA damaged by methyl methanesulfonate in bacteriophage T4.
A highly purified preparation of T4 endonuclease V does not degrade DNA alkylated with methyl methanesulfonate, and the methyl methanesulfonate sensitivity of T4 wild type and x mutant is not affected by the v mutation. Thus, T4 endonuclease V, the v gene product, does not seem to be involved in a repair or an abortive repair of methyl methanesulfonate-damaged T4 DNA. The x and y genes of T4 and the polA and the uvrD genes of Escherichia coli are concerned with the repair of methyl methanesulfonate-induced damages in T4 DNA. Since effects of the polA and the x or y mutations are additive, it is supposed that there are at least two pathways for the repair of methyl meth-anesulfonate-damaged T4 DNA, one controlled by the x and the y genes and the other in which E. coli DNA olymerase I is involved. The partial suppression of the x gene mutation at high temerature was also demonstrated.
D003090:Coliphages; D004254:DNA Nucleotidyltransferases; D004260:DNA Repair; D004269:DNA, Bacterial / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism / Q000528:radiation effects; D004720:Endonucleases / Q000378:metabolism; D004926:Escherichia coli; D005796:Genes; D008698:Mesylates; D008741:Methyl Methanesulfonate; D009154:Mutation; D011061:Poly A; D013696:Temperature; D014466:Ultraviolet Rays
D004269:DNA, Bacterial; D004279:DNA, Viral; D008698:Mesylates; D011061:Poly A; D008741:Methyl Methanesulfonate; D004254:DNA Nucleotidyltransferases; D004720:Endonucleases