Biosynthesis of unsaturated fatty acids in cultured minimal deviations hepatoma 7288 C cells.
Minimal Deviation Hepatoma 7288 C cells were cultured in confluent layer with labeled linoleic, alpha-linolenic, eicosa-8,11,14-trienoic and stearic acids. The cells in culture preserved their ability to convert stearic acid to oleic acid. They also synthesized arachidonic acid from linoleic acid or eicosa-8,11,14-trienoic acid. The conversion was very low with linoleic acid and high with eicosatrienoic acid. Eicosapentaenoic acid and other homologs of the alpha-linolenic acid family were synthesized from alpha-linolenic acid. The biosynthetic patterns were the cells was modified by the fatty acid composition of the media.
D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D002478:Cells, Cultured / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis*; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism*
D005231:Fatty Acids, Unsaturated