Antitumor effects of antibody-diphtheria toxin conjugates. II. Immunotherapy with conjugates directed against tumor antigens induced by simian virus 40.
Hamster and rabbit antibodies against antigens on the surface of simian virus 40 (SV40)-transformed hamster sarcoma or lymphoma cells were purified by immunoadsorption on SV40-transformed cells and conjugated to diphtheria toxin with glutaraldehyde. Treatment with a single dose of antibody-toxin conjugate partially protected hamsters concurrently challenged with 10(3) SV40-transformed sarcoma cells, as evidenced by a reduction in tumor incidence, an increase in tumor latency, and a prolongation of the life-spans of hamsters that developed tumors. Treatment of established sarcomas was ineffective, but repeated treatment with the conjugates induced complete regressions in 20-56% of hamsters bearing established transplants in the SV40-induced lymphoma.
D000818:Animals; D000912:Antibodies, Neoplasm / Q000008:administration & dosage; D000951:Antigens, Neoplasm; D006224:Cricetinae; D004167:Diphtheria Toxin / Q000627:therapeutic use*; D007167:Immunotherapy; D008223:Lymphoma / Q000628:therapy*; D009374:Neoplasms, Experimental / Q000628:therapy; D012513:Sarcoma, Experimental / Q000517:prevention & control* / Q000628:therapy; D013539:Simian virus 40 / Q000276:immunology*
D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D004167:Diphtheria Toxin