Morphological changes in cultured mammalian cells: prevention by the calcium ionophore A23187.
The morphological changes induced by butyrate in HeLa cells and by monobutyryl or dibutyryl cAMP in CHO cells are prevented by micromolar concentrations of the divalent cation ionophore A23187. The ionophore is unable to prevent such changes in medium from which calcium is omitted. At slightly higher (but nontoxic) concentrations, the ionophore inhibits the butyrate-mediated induction of the ganglioside biosynthetic enzyme, sialyltransferase, in HeLa. In CHO, sialyltransferase activity is normally high and not altered by any of the compounds tested.
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003994:Bucladesine / Q000494:pharmacology; D002087:Butyrates / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology*; D002118:Calcium / Q000494:pharmacology; D002264:Carboxylic Acids; D002460:Cell Line; D002478:Cells, Cultured / Q000187:drug effects*; D002999:Clone Cells; D006224:Cricetinae; D004336:Drug Antagonism; D005260:Female; D005732:Gangliosides / Q000096:biosynthesis; D006367:HeLa Cells; D006801:Humans; D007788:Lactose Synthase / Q000378:metabolism; D009363:Neoplasm Proteins / Q000096:biosynthesis; D010053:Ovary; D010784:Photomicrography; D014176:Protein Biosynthesis; D012799:Sialyltransferases / Q000378:metabolism
D000900:Anti-Bacterial Agents; D002087:Butyrates; D002264:Carboxylic Acids; D005732:Gangliosides; D009363:Neoplasm Proteins; D000001:Calcimycin; D003994:Bucladesine; D007788:Lactose Synthase; D012799:Sialyltransferases; D000469:Alkaline Phosphatase; D002118:Calcium