Comparison of virus recovery techniques for assessing the virucidal activity of disinfectant spray products.
A comparison was made of virus recovery from inoculated glass surfaces by 3 different viral recovery techniques used in spray test methods. On a total of 7, 5, and 5 observations, for the cover glass crushing, cotton swab rubbing, and microscopic slide shaking techniques, respectively, the log reduction of the median tissue culture infective dose (log TCID-50/ml) averaged 1.48, 2.57, and 2.22, the mean percentage recovery was 77.98, 61.55, and 66.94%, and the coefficient of variation was 4.37, 10.01, and 7.42%. The cover glass technique was found to be the least variable and the most effective and precise of the 3 methods.
D000998:Antiviral Agents / Q000494:pharmacology*; D004202:Disinfectants / Q000494:pharmacology*; D008722:Methods; D018139:Simplexvirus / Q000187:drug effects / Q000302:isolation & purification*; D014776:Virus Cultivation
D000998:Antiviral Agents; D004202:Disinfectants