Chromatographic conditions in the expression of corticosteroid receptor specificity.
It is shown that cytosol preparations bound with various concentrations of a steroid are necessary to reveal physicochemically distinct, heterogeneous and polymorphic receptors present in the hormone specific target organ, that these cannot be fully appreciated in one-shot experiments at suboptimal steroids levels, and that they escape detection by equilibrium binding and Scatchard analysis alone.
D000450:Aldosterone / Q000378:metabolism*; D000818:Animals; D002352:Carrier Proteins / Q000302:isolation & purification; D002845:Chromatography; D003345:Corticosterone / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D007668:Kidney / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D009928:Organ Specificity; D051381:Rats; D011956:Receptors, Cell Surface
D002352:Carrier Proteins; D011956:Receptors, Cell Surface; D000450:Aldosterone; D003345:Corticosterone