An improved quantitative micro-complement fixation test.
The technical procedures for a simple quantitative micro-complement (C) fixation test are described. Major advantages of the present technique compared with the previously described method are: a) a simple measurement of the residual hemolytic activity of C by counting the radioactivity released from 51Cr-labeled sensitized sheep erythrocytes (51Cr-EA); b) an increased sensitivity of the test, brought about by the use of a relatively small number of 51CR-EA per reaction volume; and c) an increased specificity of the test, achieved by maintaining a constant amount of C available for the specific antigen--antibody reaction.
D000256:Adenoviridae / Q000276:immunology; D000818:Animals; D000937:Antigen-Antibody Reactions; D002460:Cell Line; D002474:Cell-Free System; D002478:Cells, Cultured; D002860:Chromium Radioisotopes; D003168:Complement Fixation Tests / Q000379:methods*; D006224:Cricetinae / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology; D006168:Guinea Pigs / Q000276:immunology; D006461:Hemolysis; D007106:Immune Sera; D009374:Neoplasms, Experimental / Q000276:immunology; D011817:Rabbits / Q000276:immunology; D012756:Sheep / Q000276:immunology; D013539:Simian virus 40 / Q000276:immunology
D002860:Chromium Radioisotopes; D007106:Immune Sera