Measurement of unsaturated ""granulocyte-related'' (TC I and TC III) and ""liver-related'' (TC II) B12 binders by instant batch separation using a microfine precipitate of silica (QUSO G32).
A microfine precipitate of silica (Quso G32) almost instantly quantitatively adsorbs the polypeptide transcobalamin II from serum without adsorbing the glycoproteins transcobalamins I and III. This provides a simple, rapid, and reproducible method for the measurement of the granulocyte-related transcobalamins I and III and the liver-related transcobalamin II in serum, and is also applicable to their purification, and the separation and purification of other polypeptide-glycoprotein mixtures.
D001798:Blood Proteins / Q000032:analysis*; D002850:Chromatography, Gel; D006023:Glycoproteins / Q000032:analysis; D006098:Granulocytes; D008099:Liver; D010455:Peptides / Q000032:analysis; D011869:Radioligand Assay; D012822:Silicon Dioxide; D014155:Transcobalamins / Q000032:analysis*
D001798:Blood Proteins; D006023:Glycoproteins; D010455:Peptides; D014155:Transcobalamins; D012822:Silicon Dioxide