title: The role of glucose limitation in the regulation of the transport of glucose, gluconate and 2-oxogluconate, and of glucose metabolism in Pseudomonas aeruginosa.
abstract: The pathway of glucose metabolism in Pseudomonas aeruginosa was regulated by the availability of glucose and related compounds. On changing from an ammonium limitation to a glucose limitation, the organism responded by adjusting its metabolism substantially from the extracellular direct oxidative pathway to the intracellular phosphorylative route. This change was achieved by repression of the transport systems for gluconate and 2-oxogluconate and of the associated enzymes for 2-oxogluconate metabolism and gluconate kinase, while increasing the levels of glucose transport, hexokinase and glucose 6-phosphate dehydrogenase. The role of gluconate, produced by the action of glucose dehydrogenase, as a major inhibitory factor for glucose transport, and the possible significance of these regulatory mechanisms to the organism in its natural environment, are discussed.
mesh_terms: D000429:Alcohol Oxidoreductases / Q000378:metabolism; D000645:Ammonium Sulfate / Q000378:metabolism; D001693:Biological Transport, Active; D002474:Cell-Free System; D005942:Gluconates / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D006593:Hexokinase / Q000378:metabolism; D010770:Phosphotransferases / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000201:enzymology / Q000378:metabolism*
chemical_list: D005942:Gluconates; D000429:Alcohol Oxidoreductases; D005954:Glucosephosphate Dehydrogenase; D010770:Phosphotransferases; D006593:Hexokinase; D005947:Glucose; D000645:Ammonium Sulfate