Effects of 2-deoxyglucose, glucosamine, and mannose on cell fusion and the glycoproteins of herpes simplex virus.
2-Deoxyglucose and glucosamine were found to inhibit cell fusion caused by a syncytial mutant of herpes simplex virus and to inhibit the glycosylation of viral glycoproteins in the infected cells. The inhibition of fusion and the inhibition of glycosylation caused by 2-deoxyglucose were substantially prevented when mannose was also present during infection. When glycosylation was inhibited, three new bands were found in major glycoprotein region on sodium dodecyl sulfate-polyacrylamide gels. These bands may be precursors to the normal glycoproteins. The correlation between fusion and glycosylation in the presence of 2-deoxyglucose, glucosamine, and mannose suggests that the cells cannot fuse if their glycoproteins have a considerably reduced carbohydrate content.
D002459:Cell Fusion / Q000187:drug effects*; D046508:Culture Techniques; D003837:Deoxy Sugars / Q000494:pharmacology*; D003847:Deoxyglucose / Q000494:pharmacology*; D005690:Galactose / Q000378:metabolism; D005944:Glucosamine / Q000378:metabolism / Q000494:pharmacology*; D006023:Glycoproteins / Q000096:biosynthesis*; D008358:Mannose / Q000494:pharmacology*; D011498:Protein Precursors / Q000096:biosynthesis; D018139:Simplexvirus / Q000378:metabolism*; D014764:Viral Proteins / Q000096:biosynthesis*
D003837:Deoxy Sugars; D006023:Glycoproteins; D011498:Protein Precursors; D014764:Viral Proteins; D003847:Deoxyglucose; D005944:Glucosamine; D008358:Mannose; D005690:Galactose