Temperature-sensitive mutants of Balb/3T3 cells: description of a mutant affected in cellular and polyoma virus DNA synthesis.
A temperature-sensitive Dna- mutant (ts-2) of the mouse cell Balb/3T3 is characterized. Studies with synchronized cells indicate that the defect is in DNA synthesis itself, rather than in progress toward its initiation. ts-2 supports polyoma DNA synthesis after infection at 33degreesC but not at 38degreesC. Viral DNA synthesis begun at 33degreesC is inhibited upon shift to 38degreesC. A procedure is proposed by which viral DNA synthesis can be used to distinguish different classes of cell Dna- mutants.
D002455:Cell Division; D002460:Cell Line; D004247:DNA / Q000096:biosynthesis*; D004279:DNA, Viral / Q000096:biosynthesis*; D005796:Genes; D008239:Lysine / Q000378:metabolism; D009154:Mutation; D011120:Polyomavirus; D013696:Temperature; D013936:Thymidine / Q000378:metabolism; D014529:Uridine / Q000378:metabolism
D004279:DNA, Viral; D004247:DNA; D008239:Lysine; D013936:Thymidine; D014529:Uridine