[Quantitative determination of anhydroleandomycin and glycol in oleandomycin phosphate].
A spectrophotometric method for determination of inactive admixtures in oleandomycin phosphate-anhydrooleandomycin was developed. The method was based on measuring optical density of methanol solutions of oleandomycin and its admixtures at a wave length of 235 nm of UV-spectrum. The minimun detectable amount was 0.5 per cent. A method of thin-layer chromatography in a buffered fixed layer was developed for determination of the 2nd inactive admixture, i.e. glycole. The chromatogrammes were developed with xanthydrol. The minimum detectable amount was 3 mug.
D000812:Anhydrides / Q000032:analysis*; D002850:Chromatography, Gel; D004340:Drug Contamination; D006018:Glycols / Q000032:analysis*; D009827:Oleandomycin / Q000032:analysis*; D010710:Phosphates / Q000032:analysis; D012822:Silicon Dioxide; D013056:Spectrophotometry, Ultraviolet
D000812:Anhydrides; D006018:Glycols; D010710:Phosphates; D012822:Silicon Dioxide; D009827:Oleandomycin
Kolichestvennoe opredelenie angidrooleandomitsina i glikolia v fosfate oleandomitsina