Herpesvirus proteins: DNA polymerase and pyrimidine deoxynucleoside kinase activities in temperature-sensitive mutants of herpes simplex virus type 2.
Eleven temperature-sensitive mutants of herpes simplex virus type 2 strain HG52 were examined for ability to induce DNA polymerase activity in BHK 21/C13 cells. All mutants induced DNA polymerase at a permissive temperature, (31 degrees C) and all DNA-positive mutants at a non-permissive temperature (38 degrees C). Three DNA-negative mutants induced no DNA polymerase (ts 6, ts 9) or very little DNA polymerase (ts 11), at a non-permissive temperature, while ts 1, also DNA negative, induced a little more DNA polymerase than wild-type, often at both temperatures. The DNA polymerase induced by ts 6 at 31 degrees C was temperature-sensitive in vivo, but only slightly so in vitro. These results were confirmed immunologically and suggest that HSV-2 codes for at least part of a DNA polymerase activity, necessary for infection, and that full expression of this enzyme involves at least three viral genes.
D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D002474:Cell-Free System; D004254:DNA Nucleotidyltransferases / Q000276:immunology / Q000378:metabolism*; D004279:DNA, Viral / Q000096:biosynthesis; D004790:Enzyme Induction; D009154:Mutation; D010770:Phosphotransferases / Q000276:immunology* / Q000378:metabolism*; D018139:Simplexvirus / Q000201:enzymology* / Q000276:immunology / Q000378:metabolism; D013696:Temperature
D000956:Antigens, Viral; D004279:DNA, Viral; D010770:Phosphotransferases; D004254:DNA Nucleotidyltransferases