Rate of drug metabolism in man measured by 14CO2-breath analysis.
Exhalation of 14CO2 in breath has been used to assess the rate of hepatic demethylation of (14C-dimethyl)aminopyrine, but due to the complexity of aminopyrine metabolism the pharmacokinetics of the procedure are insufficiently understood. Therefore, studies were performed in five individuals after oral administration of (14C-methoxy)glycodiazine, a model substance with relatively simple kinetic properties. Plasma concentrations of the drug and urinary output of its metabolites measured by high pressure liquid chromatography were analysed by a two-compartment open model. The terminal disappearance of 14CO2 from breath was practically identical with the terminal disappearance of glycodiazine from plasma, which could be correlated with the plasma clearance of free glycodiazine. The mean transit time of 14C-atoms from plasma to breath was 3 h. These results contribute to the pharmacokinetic basis for use of 14C-demethylation breath tests. In particular, they are consistent with the hypothesis that 14CO2-breath analysis may be used to assess certain pharmacokinetic parameters of appropriately labelled test compounds. These parameters may not necessarily be a direct reflection of the rate of demethylation.
D000328:Adult; D000368:Aged; D001944:Breath Tests; D002245:Carbon Dioxide / Q000032:analysis*; D003640:Dealkylation; D006801:Humans; D007700:Kinetics; D008722:Methods; D008954:Models, Biological; D004364:Pharmaceutical Preparations / Q000378:metabolism*; D013449:Sulfonamides / Q000097:blood / Q000378:metabolism
D004364:Pharmaceutical Preparations; D013449:Sulfonamides; D002245:Carbon Dioxide