Natural occurrence of poly(ADP-ribosyl) histones in rat liver.
Poly(ADP-ribose) bound to histones has been isolated from rat liver. When [14C]ribose was administered intraperitoneally to rats at a dosage of 300-750 mug (100-250 muCi)/10o g, approximately 1% of the radioactivity was recovered in the acid (5% CLCCCOOH)-INSOLUBLE MATERIAL OF THE LIVER NUCLEI 2 HR AFTER INJECTION. Of the acid-insoluble radioactivity, 4.5-9% was extractable with 0.25 N HCL. Carboxymethyl-cellulose column chromatography of the HCl-extracted material revealed that the radioactivity cochromatographed with histone subfractions f1 and, to a lesser extent, f2 and f3. Part of the protein-bound radioactivity was rendered acid-soluble by treatment with either snake venom phosphodiesterase or neutral NH2OH. From the enzyme digest, 5'-AMP and psiADP-ribose [2'-(5"-phosphoribosyl)-5'-AMP] were recovered, while the NH2OH treatment yielded ADP-ribose monomer and, presumably, oligomer. These observations indicate that ADP-ribose is attached to histones in vivo and is present both as a monomer and a polymer.
D000225:Adenine / Q000378:metabolism; D000244:Adenosine Diphosphate / Q000302:isolation & purification / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000378:metabolism; D000818:Animals; D002250:Carbon Radioisotopes; D002266:Carboxymethylcellulose Sodium; D002845:Chromatography; D006657:Histones / Q000302:isolation & purification* / Q000378:metabolism; D008099:Liver / Q000032:analysis* / Q000378:metabolism; D008297:Male; D009702:Nucleoside Diphosphate Sugars / Q000302:isolation & purification*; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D011119:Polynucleotides / Q000302:isolation & purification*; D011485:Protein Binding; D051381:Rats; D012266:Ribose / Q000302:isolation & purification* / Q000378:metabolism; D012910:Snake Venoms
D002250:Carbon Radioisotopes; D006657:Histones; D009702:Nucleoside Diphosphate Sugars; D011119:Polynucleotides; D012910:Snake Venoms; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D012266:Ribose; D010727:Phosphoric Diester Hydrolases; D000225:Adenine; D002266:Carboxymethylcellulose Sodium