Protein synthesis in homologous and heterologous cell-free systems from chick embryo connective tissues.
Two homologous systems for cell-free protein synthesis from chick embryo connective tissues are described. Both the skin polysomes and the wing-leg polysomes are active in collagen synthesis, but they have different requirements for optimum protein synthesis. Protein synthesis was not dependent on tissue-specific factors, since heterologous preparations of supernatant enzymes or initiation factors were able to stimulate maximum protein synthesis with each fraction of polysomes.
D000818:Animals; D002474:Cell-Free System; D002642:Chick Embryo; D003238:Connective Tissue / Q000187:drug effects / Q000378:metabolism*; D008274:Magnesium / Q000494:pharmacology; D003012:Microbial Collagenase / Q000494:pharmacology; D009132:Muscles / Q000378:metabolism; D011132:Polyribosomes / Q000187:drug effects / Q000378:metabolism; D011188:Potassium / Q000494:pharmacology; D014176:Protein Biosynthesis / Q000187:drug effects; D012867:Skin / Q000378:metabolism
D003012:Microbial Collagenase; D008274:Magnesium; D011188:Potassium