Interaction of partially purified simian virus 40 T antigen with circular viral DNA molecules.
Mixing chromatographic fractions containing simian virus 40 (SV40) T antigen with SV40 [3H]-DNA I (double-stranded, circular, supercoiled) results in the conversion of the nucleic acid to a form that will bind to a nitrocellulose filter. Unlabeled SV40 DNA I successfully competes with this reaction. Under the conditions employed, the antigen-containing fractions bind a variety of circular, viral DNA molecules. Chromatography of the antigen in three systems reveals that the T immunoreactivity migrates with DNA binding activity. In a kinetic heat inactivation experiment, the antigenic reactivity disappears simultaneously with the DNA binding activity. The data indicate the presence of a discernible DNA binding activity in fractions containing T antigen and suggest that the T antigen is the DNA binding protein being measured.
D000937:Antigen-Antibody Reactions; D000956:Antigens, Viral / Q000302:isolation & purification; D001665:Binding Sites; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002852:Chromatography, Ion Exchange; D003090:Coliphages / Q000032:analysis; D003168:Complement Fixation Tests; D004270:DNA, Circular; D004279:DNA, Viral; D004355:Drug Stability; D004926:Escherichia coli / Q000032:analysis; D006358:Hot Temperature; D006801:Humans; D007700:Kinetics; D046911:Macromolecular Substances; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D013539:Simian virus 40 / Q000032:analysis / Q000276:immunology*
D000956:Antigens, Viral; D004270:DNA, Circular; D004279:DNA, Viral; D046911:Macromolecular Substances