title: Susceptibility of chickens to avian encephalomyelitis virus. III. Behavior of the virus in growing chicks.
abstract: A chick embryo-adapted strain of avian encephalomyelitis virus was inoculated subcutaneously and orally into 40-day-old (middle-aged) and 110-day-old (advanced-aged) chicks to examine the behavior of the virus in the chick body. In the middle-aged chicks, the virus appeared in the muscle at the site of inoculation, liver, spleen, pancreas, lumbar and cervical portions of the spinal cord, and brain 1 approximately 9 days after subcutaneous inoculation, and remained mostly in the central nervous system up to 17 days after the inoculation. The virus was found in large amounts in the muscle at the site of inoculation (10(3.1)), lumbar portion (10(2.5)) and cervical portion (10(2.1)) of the spinal cord, brain (10(1.9)), and in minute amounts in the other organs examined. It appeared in 11 of 21 organs examined. In the middle-aged chicks inoculated by the oral route, the virus was detected transiently in small amounts from esophagus, pancreas, and rectum 4 approximately 14 days after inoculation. In the advanced-aged chicks inoculated by the subcutaneous route, the virus was detected in titer of 10(2.1) approximately 10(3.0) from the muscle at the site of inoculation 2 approximately 7 days after inoculation. The virus was also found sporadically in several organs up to 17 days after inoculation. In the advanced-aged chicks inoculated by the oral route, no virus appeared in any organ, but these chicks turned to be weakly positive for neutralizing antibody in the 4th or later week after inoculation.
mesh_terms: D000818:Animals; D002642:Chick Embryo; D002645:Chickens; D004679:Encephalomyelitis / Q000382:microbiology / Q000662:veterinary*; D001350:Encephalomyelitis Virus, Avian / Q000302:isolation & purification; D004770:Enterovirus; D004769:Enterovirus Infections / Q000382:microbiology / Q000662:veterinary; D004947:Esophagus / Q000382:microbiology; D009132:Muscles / Q000382:microbiology; D011201:Poultry Diseases / Q000382:microbiology*; D012007:Rectum / Q000382:microbiology