title: [Isolation of peptide containing essential tyrosine from lobster arginine kinase].
abstract: The essential tyrosine residue of Lobster muscle arginine kinase, which is part of an antigenic determinant, has been modified by tetranitromethane. Cleavage of the S-carboxymethylated nitrated enzyme with cyanogen bromide gives rise to eight peptides, one of which containing the labelled essential tyrosyl group. Ion exchange chromatography on sulfoethyl-Sephadex C-25 in urea medium has been used with success for isolation and purification of the nitrated peptide. From its amino acid composition and end groups structure this peptide is the N-terminal fragment of the protein.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001120:Arginine / Q000378:metabolism; D009132:Muscles / Q000201:enzymology*; D008121:Nephropidae; D010446:Peptide Fragments; D010770:Phosphotransferases / Q000032:analysis*; D014443:Tyrosine / Q000032:analysis*
chemical_list: D000596:Amino Acids; D010446:Peptide Fragments; D014443:Tyrosine; D001120:Arginine; D010770:Phosphotransferases
vernacular_title: Isolement du peptide a tyrosine essentielle de l'arginine kinase de homard