Cell-mediated cytotoxicity to chemically-induced rat tumours.
Cell-mediated immune reactions against carcinogen-induced rat tumours were demonstrated by the in vitro microcytotoxicity test. Lymph-node cells from syngeneic rats immunized against individual aminoazo-dye-induced hepatomas or 3-methylcholanthrene-induced sarcomas were cytotoxic for cells of the immunizing tumour compared with the effects of normal lymph-node cells. Only a low level of cross-reactivity was detectable against target cells derived from otherr tumours. Conversely, lymph-node cells from tumour -bearing rats were cross-reactive against a range of tumour cells, suggesting that the tumour-bearer was sensitized against a common antigen(s) as well as the individually specific antigen associated with these tumours. Lymph-node cells were reactive at an early time (from day 2) after tumour implantation and their cytotoxic potential persisted until terminal stages of tumour growth. Spleen cells from tumour-bearer and tumour-immune donors also exhibited comparable cytotoxic reactivities of the same specificity as that shown by lymph-node cell preparations. These findings are discussed in relation to the development of cell-mediated immune responses in the tumour-bearing rat and in immunized rats which are capable of rejecting transplanted tumour cells.
D000818:Animals; D000937:Antigen-Antibody Reactions; D000951:Antigens, Neoplasm; D002273:Carcinogens; D006528:Carcinoma, Hepatocellular / Q000139:chemically induced / Q000276:immunology*; D002460:Cell Line; D005260:Female; D007111:Immunity, Cellular; D007114:Immunization; D008113:Liver Neoplasms / Q000139:chemically induced / Q000276:immunology*; D008198:Lymph Nodes / Q000276:immunology*; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced / Q000276:immunology*; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental; D051381:Rats; D012513:Sarcoma, Experimental / Q000139:chemically induced / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D014185:Transplantation, Isogeneic
D000951:Antigens, Neoplasm; D002273:Carcinogens