An improved and easy technique for polyamine determination in biological samples. Application to cell-free system from hypertrophied rat heart.
An accurate, improved cation-exchange chromatographic method using o-phthalaldehyde and ultraviolet detection at 280 nm for the determination of free polyamines (putrescine, spermidine, spermine) has been developed. Different samples, such as the 105,000 g supernatant of reticulocyte or heart muscle, and KCl ribosomal wash containing initiation factors, can be analysed. The minor modification of reagents results in a good precision and sensitivity, which is demonstrated by a relative standard deviation of 5--9% and recoveries of 98%. This technique is of particular interest because it allows polyamine determination in biological samples with high concentrations of salt.
D000818:Animals; D006332:Cardiomegaly / Q000378:metabolism*; D002852:Chromatography, Ion Exchange / Q000379:methods; D006321:Heart / Q000187:drug effects; D007700:Kinetics; D009206:Myocardium / Q000032:analysis / Q000378:metabolism; D010448:Peptide Initiation Factors / Q000378:metabolism; D011073:Polyamines / Q000032:analysis*; D011132:Polyribosomes / Q000378:metabolism; D011700:Putrescine / Q000032:analysis; D051381:Rats; D012156:Reticulocytes / Q000032:analysis; D012270:Ribosomes / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013095:Spermidine / Q000032:analysis; D013096:Spermine / Q000032:analysis; D014284:Triiodothyronine / Q000494:pharmacology
D010448:Peptide Initiation Factors; D011073:Polyamines; D014284:Triiodothyronine; D013096:Spermine; D013095:Spermidine; D011700:Putrescine