A simple competitive protein-binding assay for adenosine-3',5'-monophosphate in plasma and urine.
A modified competitive protein-binding assay for the measurement of adenosine-3',5'-monophosphate is described. The procedure allows measurement of adenosine-3',5'-monophosphate in unextracted plasma samples. The mean plasma values in 25 normal, fasting and ambulatory subjects were 22.7 +/- 4.7 pmol/ml (mean +/- SD) (range 13-31 pmol/ml. The mean urinary content was 3.2 +/- 1.0 mumol per g creatinine (mean +/- SD) (n=24).
D001667:Binding, Competitive; D001786:Blood Glucose / Q000032:analysis; D000242:Cyclic AMP / Q000032:analysis* / Q000097:blood / Q000652:urine; D004492:Edetic Acid; D005934:Glucagon / Q000494:pharmacology; D006801:Humans; D007328:Insulin / Q000494:pharmacology; D008722:Methods; D011485:Protein Binding
D001786:Blood Glucose; D007328:Insulin; D005934:Glucagon; D004492:Edetic Acid; D000242:Cyclic AMP