Purification of Oidiodendron kalrai proteases.
Oidiodendron kalrai yeast-phase cells demonstrate proteolytic activity. Some of the proteolytic enzymes of the crude extract were purified by a combination of ammonium sulfate precipitation, Sephadex G-200, and diethylaminoethyl (DEAE) cellulose column chromatography. At least six proteins exhibiting a range of proteolytic activities could be identified by these procedures. Purity of the enzyme fractions obtained from the DEAE-cellulose columns was tested by running polyacrylamide gels.
D000645:Ammonium Sulfate; D002364:Caseins / Q000378:metabolism; D002474:Cell-Free System; D011232:Chemical Precipitation; D002848:Chromatography, DEAE-Cellulose; D003094:Collagen / Q000378:metabolism; D004549:Elastin / Q000378:metabolism; D005780:Gelatin / Q000378:metabolism; D006454:Hemoglobins / Q000378:metabolism; D007768:Lactalbumin / Q000378:metabolism; D003012:Microbial Collagenase / Q000302:isolation & purification; D003904:Mitosporic Fungi / Q000201:enzymology*; D010447:Peptide Hydrolases / Q000302:isolation & purification* / Q000378:metabolism
D002364:Caseins; D006454:Hemoglobins; D005780:Gelatin; D003094:Collagen; D004549:Elastin; D007768:Lactalbumin; D010447:Peptide Hydrolases; D003012:Microbial Collagenase; D000645:Ammonium Sulfate