Effects of 6-aminonicotinamide on growth and acetylcholinesterase activity during differentiation of neuroblastoma cells in vitro.
Addition of 6-AN (0.01 mg/ml) to growing cultures of C-1300 neuroblastoma cells strongly reduced cell division. This growth inhibition was accompanied by a higher cell volume and a lower protein content per cell as compared to controls. Concurrently the specific activity of AChE increased markedly incontrols and 6-AN-treated cultures. During the experimental periods the specific activity of AChE was significantly higher after 6-AN. Morphologically, 6-AN-treated cultures showed characteristic signs of differentiation, i.e. enlarged, flattened cells with long branched processes. The described effect of 6-AN on growth and differentiation of neuroblastoma cells was less pronounced if cells received the antimetabolite after a subcultivation period of 5 days.
D015120:6-Aminonicotinamide / Q000494:pharmacology*; D000110:Acetylcholinesterase / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects*; D002478:Cells, Cultured; D009363:Neoplasm Proteins / Q000378:metabolism; D009447:Neuroblastoma / Q000201:enzymology*; D009536:Niacinamide / Q000031:analogs & derivatives*; D013997:Time Factors
D009363:Neoplasm Proteins; D009536:Niacinamide; D015120:6-Aminonicotinamide; D000110:Acetylcholinesterase