Comparisons of major cell-surface proteins of normal and transformed cells.
Transformation of the chick fibroblast surface has been studied in cells infected with Schmidt-Ruppin Rous sarcoma virus and the temperature-sensitive mutant of this virus, TS-68. Major findings following transformation induced by a shift from nonpermissive (41 C.) to permissive (36 C.) temperature in TS-68 infected cells were: (1) rapid cessation or slowing of the synthesis of a protein, M.W. 100-200,000, localization uncertain; (2) cessation or slowing of the synthesis of a plasma membrane protein, M.W. 45,000, within 2-4 hours; (3) cessation or slowing of the synthesis of a large trypsin- and collagenase- sensitive protein (M.W. greater than 200,000) only after an extended period of morphologic transformation. In addition, increased quantities of type-specific viral antigen in the membranes of infected cells were observed in TS-68-infected cells at 41 compared with 36 C.
D000818:Animals; D000956:Antigens, Viral; D001358:Avian Sarcoma Viruses / Q000472:pathogenicity; D002462:Cell Membrane / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D002471:Cell Transformation, Neoplastic; D002642:Chick Embryo; D005347:Fibroblasts / Q000382:microbiology / Q000502:physiology; D006868:Hydrolysis; D003012:Microbial Collagenase; D008970:Molecular Weight; D009154:Mutation; D010455:Peptides; D014176:Protein Biosynthesis; D011506:Proteins / Q000032:analysis*; D013696:Temperature; D014357:Trypsin
D000956:Antigens, Viral; D010455:Peptides; D011506:Proteins; D014357:Trypsin; D003012:Microbial Collagenase