Surface sulphydryl groups and hexose monophosphate pathway activity in resting human polymorphonuclear leucocytes.
The role of surface -SH groups on the hexose monophosphate pathway activity in resting human polymorphonuclear leucocytes was studied. Endotoxin, taurocholic acid and concanvalin A stimulated [I-14C]glucose oxidation to a greater degree than [6-14C]glucose oxidation. The stimulation of glucose-I-C-oxidation by endotoxin, taurocholic acid and concanavalin A could be prevented by p-chloromercurybenzene sulphonic acid, an inhibitor of surface -SH groups. In contrast, polymorphonuclear leucocytes from four patients with chronic granulomatous disease failed to show stimulation of glucose-I-C-oxidation by endotoxin, taurocholic acid or concanavalin A.
D002731:4-Chloromercuribenzenesulfonate / Q000494:pharmacology; D001692:Biological Transport; D001786:Blood Glucose / Q000378:metabolism; D002463:Cell Membrane Permeability / Q000187:drug effects*; D003078:Colchicine / Q000494:pharmacology; D003208:Concanavalin A / Q000494:pharmacology; D004731:Endotoxins / Q000494:pharmacology; D006105:Granulomatous Disease, Chronic / Q000097:blood; D006600:Hexosephosphates / Q000097:blood*; D006801:Humans; D037102:Lectins / Q000494:pharmacology; D009504:Neutrophils / Q000378:metabolism*; D013438:Sulfhydryl Compounds / Q000097:blood*; D013499:Surface Properties
D001786:Blood Glucose; D004731:Endotoxins; D006600:Hexosephosphates; D037102:Lectins; D013438:Sulfhydryl Compounds; D003208:Concanavalin A; D002731:4-Chloromercuribenzenesulfonate; D003078:Colchicine