The monosaccharide composition and sequence of the carbohydrate moiety of human serum low density lipoproteins.
Human serum low density lipoprotein (d = 1.027-1.045) was delipidated with organic solvents and the apoprotein digested with thermolysin. The digest was fractionated by gel filtration and DEAE-cellulose chromatography. Two glycopeptides were obtained. One of the glycopeptides (GP-I) contained 2 residues of N-acetylglucosamine and 6 residues of mannose per mole of the glycopeptide, while the other contained 2 sialic acid, 5 mannose, 2 galactose, and 3 N-acetylglucosamine residues per mole of glycopeptide. The results of sequential enzymatic digestion with purified glycosidases, periodate oxidation, and partial acid hydrolysis lead us to propose the following sturctures for the two glycopeptides: (see article). These glycopeptides represent at least 50% of the carbohydrate moiety of LDL.
D000117:Acetylglucosamine / Q000032:analysis; D000118:Acetylglucosaminidase; D000596:Amino Acids / Q000032:analysis; D001665:Binding Sites; D005690:Galactose / Q000032:analysis; D005696:Galactosidases; D006020:Glycopeptides / Q000097:blood; D006801:Humans; D008077:Lipoproteins, LDL / Q000097:blood; D008358:Mannose / Q000032:analysis; D008361:Mannosidases; D009439:Neuraminidase; D010446:Peptide Fragments / Q000032:analysis; D012794:Sialic Acids / Q000032:analysis
D000596:Amino Acids; D006020:Glycopeptides; D008077:Lipoproteins, LDL; D010446:Peptide Fragments; D012794:Sialic Acids; D005696:Galactosidases; D008361:Mannosidases; D009439:Neuraminidase; D000118:Acetylglucosaminidase; D008358:Mannose; D000117:Acetylglucosamine; D005690:Galactose