[The use of N,N'-diethylpseudoisocyaninchloride for the polarization optical detection of polyanions in the histological section (author's transl)].
In the paper a technique of the polarization optical qualitative and quantitative detection of polyanions with N,N'-diethylpseudoisocyaninchloride in the histological section is described. It is possible to prevent depolymerisation after precipitation with potassium ferricyanide and to intensify the birefringence of the histological structures. In vitro attempts and measurements of the dispersion of the double refraction and of the dichroism indicate connections between the optical appearances, furthermore it is possible to make statements about the mechanism of the arrangement of the tissue-dye-ferricyanide-complex.
D000838:Anions; D001485:Basement Membrane / Q000648:ultrastructure; D001718:Birefringence; D005260:Female; D005292:Ferricyanides; D006025:Glycosaminoglycans / Q000032:analysis; D006651:Histocytochemistry; D006801:Humans; D010920:Placenta / Q000032:analysis / Q000648:ultrastructure; D011247:Pregnancy; D011804:Quinolines
D000838:Anions; D005292:Ferricyanides; D006025:Glycosaminoglycans; D011804:Quinolines
Zum Einsatz von N,N'-Diäthylpseudoisocyaninchlorid für den polarisationsoptischen Nachweis von Polyanionen im histologischen Schnitt.