A microtiter solid-phase radioimmunoassay for hepatitis A antigen and antibody.
A microtiter solid phase radioimmunoassy for hepatitis A antigen (HA Ag) and antibody (anti-HA) was developed. The test was more sensitive than immune adherence hemagglutination for detecting HA Ag and almost as sensitive for detecting anti-HA. The specificity and sensitivity of reagents were examined and optimum conditions for the test were determined. Radioimmunoassay, immune adherence hemagglutination, and immune electron microscopy were compared for detecting anti-HA. A serologic response to HA Ag was detected in paired sera from patients with type A hepatitis but not from patients with type B or non-A, non-B hepatitis by all three techniques.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000917:Antibody Formation; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000032:analysis*; D001667:Binding, Competitive; D002500:Centrifugation, Isopycnic; D002586:Cesium; D003289:Convalescence; D006506:Hepatitis A / Q000276:immunology; D006507:Hepatovirus / Q000276:immunology*; D006801:Humans; D002679:Pan troglodytes; D011863:Radioimmunoassay / Q000379:methods; D013997:Time Factors
D000914:Antibodies, Viral; D000956:Antigens, Viral; D002586:Cesium