Assays for phosphotriester formation in the reaction of bacteriophage R17 with a group of alkylating agents.
The interaction of bacteriophage R17 with 8 compounds has been studied, comparing the contribution of degradation of ribonucleic acid to the total toxicity. Breaks in the RNA chain result from the hydrolysis of phosphotriesters and thus are a measure of the extent of O-alkylation and of the SN1-type mechanism of the reaction. With many alkylating agents mutagenicity and carcinogenicity increase with increasing SN1 character of the reaction. In experiments with methyl methanesulphonate no evidence of degradation was observed at up to 19 times the mean lethal dose (620 methylations/RNA molecule). Breaks in the RNA chain accounted for 1 in 10 of the lethal lesions with beta-hydroxyethyl methanesulphonate, 1 in 60 with bis-(2-chloromethyl)methylamine (nitrogen mustard, HN2), less than 1 in 125 with 2,2-dichlorvinyl dimethyl phosphate (dichlorovos, DDVP), and 1 in 200 with propylene oxide. The hydrolysis rate of bis-(2 chloroethyl)ether was too slow for any reaction to be detected. In reactions with the carcinogen bis-(2-chloromethyl)ether the toxicity observed could be accounted for by the formaldehyde produced on hydrolysis. Cross-linking of the bacteriophage components by formaldehyde reduced the survival range over which the physical state of the RNA could be studied. No evidence of RNA degradation was observed. Reaction of the formaldehyde led to a progressive loss of biological activity over 24 h, a loss which was partially reversed by dialysis.
D000477:Alkylating Agents / Q000494:pharmacology*; D001725:Bis(Chloromethyl) Ether / Q000494:pharmacology; D003090:Coliphages / Q000187:drug effects*; D004006:Dichlorvos / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004987:Ethers / Q000494:pharmacology; D005019:Ethyl Ethers / Q000494:pharmacology; D007700:Kinetics; D008466:Mechlorethamine / Q000494:pharmacology; D008698:Mesylates / Q000494:pharmacology; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D012367:RNA, Viral / Q000378:metabolism
D000477:Alkylating Agents; D004987:Ethers; D005019:Ethyl Ethers; D008698:Mesylates; D012367:RNA, Viral; D008466:Mechlorethamine; D001725:Bis(Chloromethyl) Ether; D004006:Dichlorvos; D008741:Methyl Methanesulfonate