title: The effect of a myelin apoprotein on lipid structure: a spin probe study.
abstract: Spin probes, stable free radical derivatives of stearic acid and cholestanone, were used to observe the effects of the "Folch-Lees" protein isolated from the white matter of bovine brain on the organization and motion of lipid molecules. The incorporation of the organic solvent soluble form of this protein decreased the tendency of a variety of lipid molecules with zero, positive or negative net charges to arrange themselves close to the normal to the lipid bilayer. The aqueous form of the protein also had a profound chaotropic effect on the molecular geometry of the lipid, but only if the lipids had a net negative charge (the protein has a net positive charge in the pH range investigated). Examination of the ESR spectra indicated that this protein altered the geometry of the lipid structure without causing major changes in the mobility of the individual lipid molecules.
mesh_terms: D000818:Animals; D001059:Apoproteins; D001665:Binding Sites; D001923:Brain Chemistry; D002417:Cattle; D002776:Cholestanes; D002778:Cholestanones; D002855:Chromatography, Thin Layer; D004530:Egg Yolk; D004578:Electron Spin Resonance Spectroscopy; D005260:Female; D008055:Lipids; D008968:Molecular Conformation; D009186:Myelin Sheath / Q000032:analysis*; D009419:Nerve Tissue Proteins; D010712:Phosphatidic Acids; D010713:Phosphatidylcholines; D011485:Protein Binding; D011487:Protein Conformation; D013113:Spin Labels
chemical_list: D001059:Apoproteins; D002776:Cholestanes; D002778:Cholestanones; D008055:Lipids; D009419:Nerve Tissue Proteins; D010712:Phosphatidic Acids; D010713:Phosphatidylcholines; D013113:Spin Labels