Suppressor cell induction and reticuloendothelial cells activation produced in the mouse by beta 1-3 glucan.
We made a sequential study of the proliferative and functional changes occurring in RE cells after beta 1-3 glucan administration in BDF1, and C57BL mouse. beta 1-3 glucan was administered by single i.v. 50 mg/kg or i.p. 15 mg/kg injection. This successively induced changes in RE cells as follows: on day 3 a rise of acid phosphatase activity in peritoneal macrophages; on day 6 an increase of 3H-TdR incorporation in spleen and peritoneal lymphocytes together with an intense suppression of PHA and LPS responses by spleen cells; on day 10 a 5-fold increase of the percentage of peroxidase rich monocytes in the peritoneum. Thereafter all the values went back to or below control. Our results indicate that beta 1-3 glucan is an in vivo mitogen and a macrophage activator.
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D001402:B-Lymphocytes / Q000276:immunology; D002455:Cell Division; D004247:DNA / Q000378:metabolism; D005260:Female; D005936:Glucans / Q000008:administration & dosage; D007274:Injections, Intraperitoneal; D007275:Injections, Intravenous; D008264:Macrophages; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012157:Mononuclear Phagocyte System / Q000166:cytology*; D009929:Organ Size; D010586:Phagocytes / Q000201:enzymology; D013154:Spleen / Q000033:anatomy & histology / Q000166:cytology; D013163:Splenomegaly; D013601:T-Lymphocytes / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000166:cytology*
D005936:Glucans; D004247:DNA