Influence of size, protein concentration, protein synthesis inhibitors,and carbon on clearance of enzymes and proteins from blood.
In order to (1) clarify the mechanism(s) for clearance and maintenance of protein levels from and in extracellular fluids, and (2) explore the possibility of interconnections between enzyme plasma levels and those of tissues, rats were injected with glutamate dehydrogenase, phosphoglycerate, encolase, carbamyl phosphate synthetase, serine dehydratase, deoxyribonuclease I, ribonuclease A, hemoglobin, and human serum albumin. A close relationship between the molecular weights of enzymes and the rates of clearance was found.
D000818:Animals; D001798:Blood Proteins / Q000378:metabolism*; D002244:Carbon / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D003851:Deoxyribonucleases / Q000097:blood; D004798:Enzymes / Q000097:blood*; D005969:Glutamate Dehydrogenase / Q000097:blood; D006454:Hemoglobins / Q000378:metabolism; D012695:L-Serine Dehydratase / Q000097:blood; D008657:Metabolic Clearance Rate; D008970:Molecular Weight; D010736:Phosphoglycerate Mutase / Q000097:blood; D010751:Phosphopyruvate Hydratase / Q000097:blood; D010770:Phosphotransferases / Q000097:blood; D011691:Puromycin / Q000494:pharmacology; D051381:Rats; D012260:Ribonucleases / Q000097:blood; D012709:Serum Albumin / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000494:pharmacology
D001798:Blood Proteins; D004798:Enzymes; D006454:Hemoglobins; D012709:Serum Albumin; D003609:Dactinomycin; D012710:Serum Albumin, Bovine; D011691:Puromycin; D002244:Carbon; D003513:Cycloheximide; D005969:Glutamate Dehydrogenase; D010770:Phosphotransferases; D003851:Deoxyribonucleases; D012260:Ribonucleases; D010751:Phosphopyruvate Hydratase; D012695:L-Serine Dehydratase; D010736:Phosphoglycerate Mutase