[Incorporation of tritiated thymidine into testicular deoxyribonucleic acid of the rat. Effect of inhibin].
tritiated thymidine is incorporated into DNA of spermatogonia type B as proved by autohistoradiography when injected in vivo three hours before the sacrifice. Maximum binding and specific activity (labelled thymidine expressed in DPM per mg DNA) are obtained in pubertal rats aged 42 days and weighting 150 g. Inhibin preparation extracted from rete testis fluid (RTF3) specifically inhibits tritiated thymidine into testicular DNA. Thus, no modification of incorporation into hepatic DNA is observed and the preparation loses its inhibitory effect when denatured by heating and trypsin digestion. Tritiated thymidine incorporation into testicular DNA is poor in normal adult rats and in pubertal hypophysectomized animals, RTF3 does not modify the thymidine incorporation in both conditions. The reasons for this lack of effect are discussed. An experimental condition of spermatogonial regeneration is obtained by testicular irradiation. Inhibin preparation inhibits the regenerative DNA synthesis.
D000375:Aging; D000818:Animals; D001345:Autoradiography; D004247:DNA / Q000096:biosynthesis*; D007016:Hypophysectomy; D008297:Male; D011506:Proteins / Q000494:pharmacology*; D051381:Rats; D013735:Testicular Hormones / Q000494:pharmacology*; D013737:Testis / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors
D011506:Proteins; D013735:Testicular Hormones; D004247:DNA; D013936:Thymidine
Etude de l'incorporation de la thymidine tritiée dans l'acide désoxyribonucléique testiculaire du Rat. Influence de l'inhibine.