Novel pathway for degradation of protocatechuic acid in Bacillus species.
A species of Bacillus, tentatively identified as B. circulans, degrades protocatechuic acid by a novel reaction involving meta-fission between C2 and C3 of the benzene nucleus. 2-Hydroxymuconic semialdehyde is then degraded to pyruvate and acetaldehyde by enzymatic reactions described in previous work. Protocatechuate 2,3-oxygenase exhibits a rather narrow substrate specificity; the methyl and ethyl esters of protocatechuic acid are oxidized, but other substrates for ring-fission oxygenases, notably catechol, gallic acid, and homoprotocatechuic acid, are not attached.
D000079:Acetaldehyde / Q000096:biosynthesis; D000447:Aldehydes / Q000096:biosynthesis; D001407:Bacillus / Q000201:enzymology / Q000378:metabolism*; D001565:Benzoates / Q000378:metabolism*; D002396:Catechols / Q000378:metabolism; D002474:Cell-Free System; D055598:Chemical Phenomena; D002621:Chemistry; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis; D005233:Fatty Alcohols / Q000096:biosynthesis; D006867:Hydrolases / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism; D010101:Oxygen Consumption; D010105:Oxygenases / Q000378:metabolism; D011549:Pseudomonas / Q000378:metabolism; D011773:Pyruvates / Q000096:biosynthesis; D013053:Spectrophotometry
D000447:Aldehydes; D001565:Benzoates; D002396:Catechols; D005231:Fatty Acids, Unsaturated; D005233:Fatty Alcohols; D011773:Pyruvates; D009243:NAD; D010088:Oxidoreductases; D010105:Oxygenases; D006867:Hydrolases; D000079:Acetaldehyde