Expression of the Escherichia coli cell division gene sep cloned in a lambda Charon phage.
The Escherichia coli cell division gene sep, which probably codes for one of the penicillin-binding proteins, has been cloned into lambda Charon 10 to form a viable sep+ transducing phage. After infection with this hybrid phage, penicillin-binding protein 3 was overproduced and incorporated into the E. coli inner membrane.
D001426:Bacterial Proteins / Q000235:genetics; D010582:Bacteriophage lambda / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics; D002455:Cell Division; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D008565:Membrane Proteins / Q000235:genetics; D014161:Transduction, Genetic
D001426:Bacterial Proteins; D002352:Carrier Proteins; D004274:DNA, Recombinant; D008565:Membrane Proteins