Effect of ascorbic acid on rhinovirus replication in WI-38 cells.
Exposure of WI-38 cells to ascorbic acid plus glutathione mixtures in growth medium for 2 days prior to infection with rhinovirus serotype 20 and during virus replication suppressed multicyclic but not single-cycle growth of the virus. Multicyclic growth was suppressed over the range of multiplicity of infection (m.o.i.) of 4 times 10-minus 1 minus 4 times 10-minus 4 PFU/cell. There was some suggestion that, in the presence of ascorbic acid plus glutathione, interferon was produced at the highest m.o.i. tested but at barely detectable levels.
D001205:Ascorbic Acid / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D005978:Glutathione / Q000494:pharmacology; D006801:Humans; D007372:Interferons / Q000032:analysis; D012229:Rhinovirus / Q000187:drug effects*; D010948:Viral Plaque Assay; D014779:Virus Replication / Q000187:drug effects
D007372:Interferons; D005978:Glutathione; D001205:Ascorbic Acid