Studies on poison ivy. In vitro lymphocyte transformation by urushiol-protein conjugates.
The isolation and purification of poison ivy urushiol is described. The preparation of urushiol-ski protein and urushiol human serum albumin is also described. Lymphocytes from eleven donor naturally sensitized to poison ivy and from four non-sensitive individuals have been cultured for 5 days in the presence of urushiol-carrier conjugates. Lymphocytes from seven of the eleven sensitive donors responded with a stimulation index greater than 3.0 to urushiol-albumin conjugate. When urushiol-skin protein conjugate was used as a stimulant, lymphocytes from only three of the eleven sensitive donors responded. The results suggest that urushiol-protein conjugates can stimulate sensitive lymphocytes in vitro, although a response is not observed in every individual naturally sensitized to poison ivy.
D000328:Adult; D000485:Allergens / Q000276:immunology* / Q000302:isolation & purification; D002396:Catechols / Q000276:immunology* / Q000302:isolation & purification; D002849:Chromatography, Gas; D011040:Dermatitis, Toxicodendron / Q000276:immunology*; D005260:Female; D006801:Humans; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D010947:Plants, Toxic; D011506:Proteins; D012709:Serum Albumin; D012867:Skin; D013936:Thymidine / Q000378:metabolism; D012251:Toxicodendron / Q000032:analysis
D000485:Allergens; D002396:Catechols; D011506:Proteins; D012709:Serum Albumin; C003747:urushiol; D013936:Thymidine