The influence of some fractions of egg yolk on the survival of ram spermatozoa at 5 degrees C.
Ram spermatozoa were stored at 5 degrees C in diluents containing various fractions of egg yolk prepared by dialysis, ultrafiltration and ion-exchange chromatography. They survived storage best in the presence of components of egg yolk which were non-dialysable and were not filtered through membranes which retained substances of molecular weight greater than 100 000. The substances isolated in peak B of the ion-exchange chromatogram of whole egg yolk described by Seideman et al. (1969) gave greater protection than those from other fractions from this chromatographic system. These data indicate that the low-density lipoprotein fraction of egg yolk is the most likely source of protection to ram spermatozoa against the effects of storage at 5 degrees C.
D000704:Analysis of Variance; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002852:Chromatography, Ion Exchange; D003080:Cold Temperature; D003956:Dialysis; D004527:Egg Proteins / Q000494:pharmacology; D004530:Egg Yolk; D005260:Female; D008077:Lipoproteins, LDL / Q000494:pharmacology; D008297:Male; D008970:Molecular Weight; D012756:Sheep / Q000502:physiology*; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects; D014462:Ultrafiltration
D004527:Egg Proteins; D008077:Lipoproteins, LDL