Disappearance of insulin response after enzymatic treatment of sodium-transporting amphibian epithelia.
Amphibian epithelia specialized in trans-cellular sodium transport lose their capacity to react to insulin by a stimulation of this process upon treatment with collagenase; baseline activity and responsiveness to other hormones (vasopressin, aldosterone) bringing about such a stimulation are preserved. This renders it likely that proteases contaminating most collagenase preparations exert a detrimental effect on the receptors held responsible for interaction between insulin and its target cells in the tissues examined.
D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D002024:Bufo marinus; D004848:Epithelium / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000378:metabolism*; D003012:Microbial Collagenase / Q000494:pharmacology*; D011896:Rana temporaria; D011956:Receptors, Cell Surface / Q000187:drug effects; D012867:Skin / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism*
D007328:Insulin; D011956:Receptors, Cell Surface; D012964:Sodium; D003012:Microbial Collagenase