Phosphorylation of the lysine-rich histones throughout the cell cycle.
The phosphorylating of the lysine-rich histone at various stages in the cell cycle has been studied. In rapidly dividing cell populations the lysine-rich histone is phosphorylated rapidly after synthesis and more slowly once bound to the chromosome. The half-life of hydrolysis of such interphase phosphorylation in 5 hr except during mitosis when the phosphata hydrolysis increases almost three-fold. During mitosis there is extensive phosphorylation at sites different from those phosphorylated during interphase and a smaller measure of sites common to both mitotic and interphase cells. The sites of mitotic phosphorylation are most critically distinguished from those phosphorylated in interphase by the rapidly hydrolysis of M-phase phosphohistone when the cells divide and enter the G1 phase of the cell cycle.
D001345:Autoradiography; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D002478:Cells, Cultured; D004273:DNA, Neoplasm / Q000096:biosynthesis; D003703:Demecolcine / Q000494:pharmacology; D004586:Electrophoresis; D006657:Histones / Q000096:biosynthesis*; D006918:Hydroxyurea / Q000494:pharmacology; D008113:Liver Neoplasms; D008239:Lysine; D008938:Mitosis; D009374:Neoplasms, Experimental / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D010750:Phosphoproteins / Q000096:biosynthesis; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D010761:Phosphorus Radioisotopes; D014316:Tritium
D004273:DNA, Neoplasm; D006657:Histones; D010710:Phosphates; D010750:Phosphoproteins; D010761:Phosphorus Radioisotopes; D014316:Tritium; D010744:Phosphoric Monoester Hydrolases; D008239:Lysine; D006918:Hydroxyurea; D003703:Demecolcine