[Duration of possible detection of cysts of Entamoeba histolytica in stool samples (author's transl)].
Stool samples containing large numbers of cysts of Entamoeba histolytica were stored at room temperature (+22 degrees C - +25 degrees C) and at +4 degrees C respectively. After 1 h, 6 h, 12 h, 20 h, 36 h, 5 d, 7 d, 10 d, 15 d, 20 d, and 30 d smears were performed and stained according to the method of HEIDENHAIN. In both groups cysts remained easily detectable for about one week; after that time, however, distinct changes of the morphology of the cysts occurred and the number of detectable individuals decreased rapidly. After 30 days storage of the stool cysts could be recognized only exceptionally and only in samples stored at 4 degrees C. At that time the microscopic picture of the smears had undergone heavy changes; yeasts predominated over bacteria. In addition it was shown, that freezing of stoll samples (at - 20 degrees C and -80 degrees C) destroyed practically all cysts.
D000818:Animals; D004748:Entamoeba histolytica / Q000302:isolation & purification* / Q000648:ultrastructure; D005243:Feces / Q000382:microbiology*; D005615:Freezing; D006801:Humans; D011309:Preservation, Biological; D013696:Temperature; D013997:Time Factors
Nachweisbarkeitsdauer von Zysten der Entamoeba histolytica in Stuhlproben