[Identification of a hemadsorbing agent discovered in uninfected mouse L cell cultures and also the same cultures chronically infected with Sindbis virus].
Electron microscope examinations of continuous lines of mouse L cells, both uninfected (L-init) and chronically infected with Sindbis virus (L-SV) revealed accumulations of ribonucleoprotein strands and virions corresponding by their parameters to paramyxoviruses in the cytoplasms of the cells. Further studies showed L-init and L-SV cell lines to have a manifest hemadsorption effect which could be completely inhibited by antiserum to parainfluenza SV5 virus. Immunofluorescence procedures detected intensive fluorescence in the cytoplasm of these cells which was observed only after treatment of the cells with antiserum to SV5 virus. In response to inoculation of cell homogenates of continuous L-init and L-SV cultures guinea pigs developed antihemagglutinating antibody to simian parainfluenza SV5 virus. On the basis of these results it may be assumed that virus-specific structures and viruses identical by their parameters to paramyxoviruses observed in electron microscope examinations of continuous mouse L-init and L-SV cells are simian parainfluenza SV5 virus.
D000818:Animals; D003593:Cytoplasm / Q000382:microbiology; D066298:In Vitro Techniques; D007739:L Cells / Q000382:microbiology*; D051379:Mice; D018108:Respirovirus / Q000302:isolation & purification*; D012845:Sindbis Virus / Q000254:growth & development*
Identifikatsiia gemadsorbiruiushchego agenta, obnaruzhennogo v neinfitsirovannoĭ, a takzhe khronicheski infitsirovannoĭ virusom Sindbis perevivaemoĭ linii myshinykh kletok L