The lact of antiviral effect of (polyinosinic acid): (polycytidylic acid) when attached to insoluble supports.
A local antiviral effect can be observed when (poly rI)-(poly rC), bound to Visking discs by u.v. irradiation, is incubated with monolayers of human foreskin fibroblast cells. Radioactive labelling of cytosine residues in (poly rI)-(poly rC) with -125I, has provided a much more sensitive method for determining the fate of the insoluble (poly rI)-(poly rC) than has been available hitherto. The antiviral effect is not related to the amount of (poly rI)-(POLY RC) present on the insoluble support but rather to the amount of polynucleotide lost from the support during incubation. Treatment of (poly rI)-(poly rC) which had been bound to cyanogen bromide-activated Sepharose with eigher dilute alkali or pancreatic ribonuclease released virtually all the polynucleotide. A small amount of (poly rI)-(poly rC) is released from the insoluble matrix in the presence of serum-free Minimum Eagle's Medium.
D000998:Antiviral Agents; D002460:Cell Line; D002476:Cellophane; D005347:Fibroblasts; D006801:Humans; D007457:Iodine Radioisotopes; D008297:Male; D008851:Micropore Filters; D010179:Pancreas / Q000201:enzymology; D010413:Penis; D011070:Poly I-C / Q000494:pharmacology*; D012260:Ribonucleases; D012685:Sepharose; D012972:Sodium Hydroxide; D014466:Ultraviolet Rays; D014721:Vesicular stomatitis Indiana virus / Q000254:growth & development; D010948:Viral Plaque Assay
D000998:Antiviral Agents; D007457:Iodine Radioisotopes; D012972:Sodium Hydroxide; D002476:Cellophane; D012685:Sepharose; D012260:Ribonucleases; D011070:Poly I-C