title: Enzyme-immunoassays for antibodies in measles, cytomegalovirus infections and after rubella vaccination.
abstract: Enzyme-immunoassays using an indirect method with alkaline phosphatase conjugated antiglobulins were satisfactory for detection of antibody to Measles and Cytomegalovirus. The antigen was passively adsorbed to polystyrene micro-harmagglutination plates for the assays. IgM antibody to Rubella was also detected by enzyme-immunoassay at 7 and 28 days after vaccination in a person who had negative Rubella serology before vaccination. IgG antibody was detected at those times in another patient who had positive Rubella serology prior to vaccination. The enzyme immunoassays appear to have the potential for routine laboratory use for virological diagnosis.
mesh_terms: D000469:Alkaline Phosphatase; D000914:Antibodies, Viral / Q000032:analysis*; D003586:Cytomegalovirus Infections / Q000276:immunology*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007075:Immunoglobulin M / Q000032:analysis; D008457:Measles / Q000276:immunology*; D012409:Rubella / Q000276:immunology*; D014611:Vaccination
chemical_list: D000914:Antibodies, Viral; D007075:Immunoglobulin M; D000469:Alkaline Phosphatase