Activation of renal erythropoietic factor by phosphorylation.
Renal erythropoietic factor (REF) serves as a substrate for a cyclic nucleotide-dependent protein kinase. The phosphorylation of REF is associated with increased erythropoietic activity. Conversely, REF isolated from hypoxic rats is a poor phosphate acceptor. These findings and the presence of a positive correlation between erythropoietin levels and urinary excretion of adenosine 3',5' monophosphate (cyclic-AMP) in anemic individuals suggest that the cyclic-AMP-protein kinase system plays an important role in the activation of REF.
D000740:Anemia / Q000378:metabolism; D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism / Q000652:urine; D004921:Erythropoietin / Q000096:biosynthesis* / Q000378:metabolism; D006801:Humans; D000860:Hypoxia / Q000378:metabolism; D007668:Kidney / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism*; D011494:Protein Kinases / Q000378:metabolism; D051381:Rats
D010710:Phosphates; D004921:Erythropoietin; D000242:Cyclic AMP; D011494:Protein Kinases