Transmissible gastroenteritis (TGE) of swine: effect of age of swine testes cells culture monolayers on plaque assays of TGE virus.
A continuous line of swine testes cell culture monolayers was infected at various ages with both cell culture-adapted transmissible gastroenteritis (TGE) virus and tissue infected with TGE virus. Both produced increasing numbers of plaques as the cell monolayers aged from two to five days. Therefore, allowing the swine testes cell monolayer to age five to six days before inoculation should increase the likelihood of detecting TGE virus by plaque assay.
D000818:Animals; D002478:Cells, Cultured; D003332:Coronaviridae / Q000254:growth & development*; D008297:Male; D013552:Swine; D013737:Testis; D013997:Time Factors; D005760:Transmissible gastroenteritis virus / Q000254:growth & development*; D010948:Viral Plaque Assay