Altered hexose transport and salt sensitivity in cyclic adenosine 3',5'-monophosphate-deficient Escherichia coli.
A cyclic adenosine 3',5'-monophosphate (cAMP)-deficient mutant strain of Escherichia coli K-12 was studied to determine the effect this cyclic nucleotide has on the overall growth and metabolism of this organism. Deficient cells were found to be more susceptible to growth inhibition by salts than were their cAMP-sufficient counterparts. The deficient cells transported alpha-methylglucoside by passive diffusion, whereas the parental cells or mutant cells grown in the presence of exogenous cAMP were able to take up alpha-methylglucoside by the normal active transport process. When viewed together with earlier studies conducted on cAMP-deficient cells, these findings support the view that cAMP plays a key role in regulating the construction and operation of the E. coli membrane system.
D000643:Ammonium Chloride / Q000494:pharmacology; D001693:Biological Transport, Active; D000242:Cyclic AMP / Q000096:biosynthesis*; D004058:Diffusion; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D006601:Hexoses / Q000378:metabolism*; D008759:Methylglycosides / Q000378:metabolism; D009154:Mutation; D010710:Phosphates / Q000494:pharmacology; D011189:Potassium Chloride / Q000494:pharmacology; D012492:Salts / Q000494:pharmacology*; D012965:Sodium Chloride / Q000494:pharmacology
D006601:Hexoses; D008759:Methylglycosides; D010710:Phosphates; D012492:Salts; D000643:Ammonium Chloride; D012965:Sodium Chloride; D011189:Potassium Chloride; D000242:Cyclic AMP