[Presence of various protein kinases and of partially copurified endogenous substrates in the soluble fraction of an experimental tumor of the rat thyroid gland].
Various fractions containing protein-kinases have been partly purified from supernatant (105 000 g) of an experimental rat's thyroid cancer (1-8 Wollman). These fractions are activated at different levels by cyclic nucleotides and present an unequal affinity for protamine; they phosphorylate an endogenous substrate apparently copurified with the enzyme fractions and adorbed with them on calcium phosphate gel.
D000818:Animals; D000242:Cyclic AMP / Q000494:pharmacology; D006152:Cyclic GMP / Q000494:pharmacology; D009374:Neoplasms, Experimental / Q000201:enzymology; D011479:Protamines; D011494:Protein Kinases / Q000032:analysis*; D051381:Rats; D013347:Subcellular Fractions; D013964:Thyroid Neoplasms / Q000201:enzymology*
D011479:Protamines; D000242:Cyclic AMP; D011494:Protein Kinases; D006152:Cyclic GMP
Sur la présence de multiples protéine-kinases et de substrats endogènes partiellement copurifiés dans la fraction soluble d'une tumeur expérimentale de thyroïde de rat