Effect of 2-deoxy-D-glucose on herpesvirus-induced inhibition of cellular DNA synthesis.
In pseudorabies virus-infected cells host DNA synthesis is turned off 4 to 5 h postinfection. In the presence of 0.5 mM 2-deoxy-D-glucose, however, synthesis of both cellular and viral DNA proceeds unimpaired throughout the virus replication cycle. The uptake of radioactive thymidine into mock-infected cells is not altered in the presence of 2-deoxy-D-glucose. Virus-specific protein synthesis and particle formation also proceed in medium containing the deoxy sugar, but the virus particles produced are noninfectious and cell fusion is inhibited.
D000818:Animals; D002459:Cell Fusion / Q000187:drug effects; D002642:Chick Embryo; D046508:Culture Techniques; D004247:DNA / Q000096:biosynthesis*; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis; D003837:Deoxy Sugars / Q000494:pharmacology*; D003847:Deoxyglucose / Q000494:pharmacology*; D006564:Herpesviridae / Q000254:growth & development*; D011558:Herpesvirus 1, Suid / Q000254:growth & development* / Q000378:metabolism; D007668:Kidney; D007930:Leucine / Q000378:metabolism; D011817:Rabbits; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014764:Viral Proteins / Q000096:biosynthesis; D014779:Virus Replication / Q000187:drug effects
D004279:DNA, Viral; D003837:Deoxy Sugars; D014764:Viral Proteins; D014316:Tritium; D004247:DNA; D003847:Deoxyglucose; D007930:Leucine; D013936:Thymidine