Minimal growth requirements for Clostridium perfringens and isolation of auxotrophic mutants.
The minimal growth requirements for two strains of Clostridium perfringens were defined, and both synthetic and semisynthetic plating media were developed. Plate counts of the wild-type strains on both of these minimal media were equivalent to those on complex media. A number of auxotrophic mutants of each strain were isolated, and their phenotypes were defined.
D000225:Adenine / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D000693:Anaerobiosis; D003016:Clostridium perfringens / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D003470:Culture Media; D007785:Lactose / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D009153:Mutagens; D009154:Mutation; D009539:Nicotinic Acids / Q000378:metabolism; D009604:Nitrosoguanidines; D010641:Phenotype; D011392:Proline / Q000378:metabolism; D012492:Salts / Q000378:metabolism; D013831:Thiamine / Q000378:metabolism; D013864:Thioglycolates; D014498:Uracil / Q000378:metabolism; D014815:Vitamins / Q000378:metabolism
D000596:Amino Acids; D003470:Culture Media; D009153:Mutagens; D009539:Nicotinic Acids; D009604:Nitrosoguanidines; D012492:Salts; D013864:Thioglycolates; D014815:Vitamins; D014498:Uracil; D011392:Proline; D007785:Lactose; D000225:Adenine; D008239:Lysine; D013831:Thiamine