title: A putative glucocorticoid receptor and a transcortin-like macromolecule in pituitary cytosol.
abstract: This study describes the binding of [3H]corticosterone and [3H] dexamethasone to soluble macromolecules in the pituitaries of adrenalectomized rats perfused at sacrifice to remove blood contamination. (1) Unlabeled dexamethasone competes only slightly for [3H] corticosterone binding when the steroids are added to cytosol as well as when the steroids are present from the time of disruption of the tissue. In the latter case, corticosterone is as good a competitor as dexamethasone for [3H]dexamethasone binding. (2) Gel permeation chrmatography with BioRad A-5M reveals the presence of a [3H] corticosterone-macromolecular complex with an elution volume comparable to transcortin as well as a very large hormone-binding complex eluting in the void volume of the column...
mesh_terms: D000315:Adrenalectomy; D000818:Animals; D001665:Binding Sites; D001667:Binding, Competitive; D002846:Chromatography, Affinity; D003345:Corticosterone / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D003907:Dexamethasone / Q000378:metabolism*; D008297:Male; D010902:Pituitary Gland / Q000378:metabolism*; D011485:Protein Binding; D051381:Rats; D011956:Receptors, Cell Surface; D014156:Transcortin / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D011956:Receptors, Cell Surface; D003907:Dexamethasone; D014156:Transcortin; D003345:Corticosterone