Effect of Xenopus laevis oocyte extract on supercoiled simian virus 40 DNA: formation of complex DNA.
A soluble cell-free extract derived from stage 6 Xenopus laevis oocytes is described. From supercoiled simian virus 40 DNA the extract produces nicked circles (having a single-strand scission), linear molecules of full unit size, shorter length fragments, and various forms of complex DNA.
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D002474:Cell-Free System; D003593:Cytoplasm / Q000378:metabolism; D004261:DNA Replication; D004270:DNA, Circular / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism*; D003854:Deoxyribonucleotides / Q000378:metabolism; D005260:Female; D006150:Guanine Nucleotides / Q000378:metabolism; D008854:Microscopy, Electron; D009690:Nucleic Acid Conformation; D009865:Oocytes / Q000378:metabolism* / Q000648:ultrastructure; D010063:Ovum / Q000378:metabolism*; D013539:Simian virus 40; D014981:Xenopus
D004270:DNA, Circular; D004279:DNA, Viral; D003854:Deoxyribonucleotides; D006150:Guanine Nucleotides