A manganese catalyzed covalent binding of estradiol - 17 beta to cytosol proteins of rabbit uterus.
Studies of the temperature sensitivity of estradiol receptor binding in rabbit uterine cytosol have revealed the existence of an enzyme which catalyzes the covalent binding of estradiol to cytosol proteins. A fraction, prepared by chromatography on Biogel P-200 and incubated at 37 degrees C in the presence of Mn++, exhibited a time-dependent, temperature-sensitive, oxidative binding of estradiol not seen in the crude cytosol preparation. Although the activity of this enzyme was shown to be independent of estradiol binding by the high affinity estrogen receptor, its presence may complicate studies of estrogen receptor action which involve the use of elevated temperatures.
D000818:Animals; D001665:Binding Sites; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D008345:Manganese / Q000494:pharmacology*; D011506:Proteins / Q000378:metabolism*; D011817:Rabbits; D011956:Receptors, Cell Surface; D014599:Uterus / Q000187:drug effects / Q000378:metabolism*
D011506:Proteins; D011956:Receptors, Cell Surface; D008345:Manganese; D004958:Estradiol