The purification and properties of superoxide dismutase from a blue-green alga.
Soluble extracts of Plectonema boryanum have been shown to contain a single, electrophoretically distinct, superoxide dismutase. The enzyme has been isolated and has been found to be an iron-containing enzyme similar to that described from the periplasm of Escherichia coli. It contains 1 Fe3+/mole of enzyme. The molecular weight was approximately 36 500, and the enzyme appeared to be composed of two  subunits of equal size joined by non-covalent interactions. ESR data are presented, as are the results of amino acid analysis.
D000596:Amino Acids / Q000032:analysis; D001665:Binding Sites; D002241:Carbohydrates / Q000032:analysis; D000458:Cyanobacteria / Q000201:enzymology*; D004578:Electron Spin Resonance Spectroscopy; D004591:Electrophoresis, Polyacrylamide Gel; D007501:Iron / Q000032:analysis; D046911:Macromolecular Substances; D008970:Molecular Weight; D011485:Protein Binding; D011487:Protein Conformation; D013056:Spectrophotometry, Ultraviolet; D013482:Superoxide Dismutase / Q000302:isolation & purification / Q000378:metabolism; D014461:Ultracentrifugation
D000596:Amino Acids; D002241:Carbohydrates; D046911:Macromolecular Substances; D007501:Iron; D013482:Superoxide Dismutase