The enhanced production of hyaluronic acid by cultured rat fibroblast cells treated with cyclic AMP and its dibutyryl derivative.
Cells of a newly established rat fibroblast line (SEN) in culture synthesize mucopolysaccharides, which have been identified as hyaluronic acid, chondroitin-4-sulfate and heparan sulfate. Treatment of the cells with adenosine 3':5'-cyclic monophosphate resulted in a marked stimulation of production of hyaluronic acid, but not of the other mucopolysaccharides. Treated cells also showed increased activity of hyaluronic acid synthetase, a reduction in growth rate, and morphological alteration. In addition, 5-bromodeoxyuridine was found to counteract greatly the cyclic AMP effect.
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000249:Adenosine Monophosphate / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000494:pharmacology; D001973:Bromodeoxyuridine / Q000494:pharmacology; D003994:Bucladesine / Q000494:pharmacology*; D002087:Butyrates / Q000494:pharmacology; D002452:Cell Count; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002809:Chondroitin Sulfates / Q000096:biosynthesis; D000242:Cyclic AMP / Q000494:pharmacology*; D006152:Cyclic GMP / Q000031:analogs & derivatives / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005965:Glucuronates / Q000096:biosynthesis; D014453:Glucuronosyltransferase / Q000378:metabolism; D006025:Glycosaminoglycans / Q000096:biosynthesis; D006497:Heparan Sulfate / Q000096:biosynthesis; D006820:Hyaluronic Acid / Q000096:biosynthesis*; D011458:Prostaglandins E / Q000494:pharmacology; D013268:Stimulation, Chemical; D013806:Theophylline / Q000494:pharmacology
D000244:Adenosine Diphosphate; D000249:Adenosine Monophosphate; D000255:Adenosine Triphosphate; D001973:Bromodeoxyuridine; D003994:Bucladesine; D002087:Butyrates; D002809:Chondroitin Sulfates; D000242:Cyclic AMP; D006152:Cyclic GMP; D005965:Glucuronates; D014453:Glucuronosyltransferase; D006025:Glycosaminoglycans; D006497:Heparan Sulfate; D006820:Hyaluronic Acid; D011458:Prostaglandins E; D013806:Theophylline