Development of a cell culture line of bovine fetal endometrial cells.
Monolayers of bovine fetal endometrial cells were established as primary culture cells within 1 to 2 weeks. After the 2nd passage, these cells were inoculated with bovine viral diarrhea virus. Effects of the virus were observed each day with a light microscope. Specific cytopathic effects consisting of degeneration and sloughing of the cells and a well-defined pattern of cytoplasmic vacuolation were observed at 5 days after inoculation.
D000818:Animals; D002417:Cattle; D002460:Cell Line; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D001908:Diarrhea Viruses, Bovine Viral / Q000254:growth & development*; D004717:Endometrium; D005260:Female; D005333:Fetus; D011247:Pregnancy; D012328:RNA Viruses / Q000254:growth & development*