title: Biochemical changes in the rat brain associated with dinitrophenol-induced brain edema.
abstract: The present paper was designed to the study of cerebral edema induced by intracarotid infusion of dinitrophenol. The determinations included variations in three lysosomal enzymes (acid phosphatase, cathepsin C and beta-glucuronidase), Na+-K+-ATP-ase, changes in cerebral RNA and protein concentrations and the synthesis of these macromolecules in vitro. In experimental brain edema a drastic drop in the activity of lysosomal enzymes took place. The acid phosphatase decreased to less than 30% of controls. Cathepsin C and beta-glucuronidase were reduced about 30% and 50% of control levels respectively. Protein concentration in the cerebral tissue also decreased by more than 50%. The concentration of RNA, RNA synthesis, and the level of Na+-K+-ATP-ase remained unchanged. Protein synthesis was stimulated by 75% (against controls). All these phenomena were suppressed when the animals subjected to the action of dinitrophenol were concomitantly treated with the antiacidotic substance, tris (hydroxymethyl) aminomethane.
mesh_terms: D000135:Acid Phosphatase / Q000032:analysis; D000251:Adenosine Triphosphatases / Q000032:analysis; D000818:Animals; D001921:Brain / Q000201:enzymology; D001923:Brain Chemistry; D001929:Brain Edema / Q000139:chemically induced / Q000473:pathology*; D002403:Cathepsins / Q000032:analysis; D004140:Dinitrophenols; D005966:Glucuronidase / Q000032:analysis; D008247:Lysosomes / Q000201:enzymology; D012313:RNA / Q000032:analysis; D051381:Rats
chemical_list: D004140:Dinitrophenols; D012313:RNA; D000135:Acid Phosphatase; D005966:Glucuronidase; D002403:Cathepsins; D000251:Adenosine Triphosphatases