Histochemical and structural changes in human myocardial cells after cardiopulmonary bypass.
The histochemical and ultrastructural effects of extracorporeal circulation and aortic cross-clamping during coronary heart surgery have been examined in drill biopsy samples of the left ventricle in 22 patients. The biopsies were obtained before and after bypass with a DeSoutter drill. Histochemical studies indicated definite differences between control and experimental biopsies, with increased succinic dehydrogenase, cytochrome oxidase, and LDH activity, while phosphorylase A and myosin ATPase activities declined. Furthermore, free phospholipid levels increased, as determined by the acid hematein reaction. Ultrastructural examination demonstrated loss of glycogen, intracellular swelling, and mitochondrial damage, which included loss of matrix density, loss of cristae, and eventual disruption in the postbypass biopsy. These results, which closely resemble the effects of ischemia and reperfusion observed in animal experiments, suggest that the initial insult is a change in membrane permeability regulation.
D000251:Adenosine Triphosphatases / Q000378:metabolism; D002315:Cardiopulmonary Bypass / Q000009:adverse effects*; D003576:Electron Transport Complex IV / Q000378:metabolism; D005112:Extracorporeal Circulation / Q000009:adverse effects*; D005260:Female; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D008931:Mitochondria, Muscle / Q000648:ultrastructure; D008995:Monoamine Oxidase / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism / Q000648:ultrastructure; D010743:Phospholipids / Q000378:metabolism; D006005:Phosphorylases / Q000378:metabolism; D013385:Succinate Dehydrogenase / Q000378:metabolism
D010743:Phospholipids; D007770:L-Lactate Dehydrogenase; D013385:Succinate Dehydrogenase; D008995:Monoamine Oxidase; D003576:Electron Transport Complex IV; D006005:Phosphorylases; D000251:Adenosine Triphosphatases