title: Ionic charge on phospholipids and their interaction with the mitochondrial adenosine triphosphatase.
abstract: The activity of the lipid-depleted, oligomycin-sensitive mitochondrial ATPase has been measured in the presence of liposomes prepared from mixtures of phosphatidylglycerol and phosphatidylglycerol lysine. Enzyme activity increased linearly with an increase in the negative charge of liposomes prepared from the phosphatidylglycerol-phosphatidylglycerol lysine mixtures. The electrophoretic mobility and activating capacity of liposomes of several other phospholipids were determined. A linear relationship between electrophoretic mobility of the liposomes and oligomycin-sensitive activity was again apparent. These observations demonstrate that the activity of the ATPase is directly proportional to the ionic charge on phospholipid activators if the acyl chain composition of the phosphoglycerides is relatively constant.
mesh_terms: D000251:Adenosine Triphosphatases; D004586:Electrophoresis; D007477:Ions; D008081:Liposomes; D008928:Mitochondria / Q000201:enzymology*; D009840:Oligomycins / Q000494:pharmacology; D010715:Phosphatidylglycerols
chemical_list: D007477:Ions; D008081:Liposomes; D009840:Oligomycins; D010715:Phosphatidylglycerols; D000251:Adenosine Triphosphatases