Screening procedure for complementation-dependent mutants of vesicular stomatitis virus.
To isolate new types of vesicular stomatitis virus (VSV) mutants, a four-stage screen was developed which identifies and characterizes mutants capable of complementing the defect in the VSV temperature-sensitive mutant tsG11. Two types of mutants of VSV, Indiana serotype, have been found by using the screen; they are new temperature-sensitive mutants which are, of necessity, not in complementation group I and mutants which do not produce plaques under conditions of single infection at 31 C (the normal permissive temperature) and are, therefore, called complementation-dependent mutants. The newly isolated, temperature-sensitive mutants fall into three complementation groups, two of which are congruent with known complementation groups; the newly identified group extends to six the number of complementation groups of VSV Indiana. The nature of the complementation-dependent mutants has not been established, but one was shown to not contain a significant deletion in its nucleic acid.
D000818:Animals; D002499:Centrifugation, Density Gradient; D005472:Fluorouracil; D005816:Genetic Complementation Test; D006378:Helper Viruses / Q000254:growth & development; D007739:L Cells; D051379:Mice; D009153:Mutagens; D009154:Mutation; D009604:Nitrosoguanidines; D009608:Nitrous Acid; D013696:Temperature; D014721:Vesicular stomatitis Indiana virus / Q000254:growth & development / Q000302:isolation & purification; D010948:Viral Plaque Assay
D009153:Mutagens; D009604:Nitrosoguanidines; D009608:Nitrous Acid; D005472:Fluorouracil