Sequence position of 3-hydroxyproline in basement membrane collagen. Isolation of glycyl-3-hydroxyprolyl-4-hydroxyproline from swine kidney.
The position of 3-hydroxyproline was investigated in the triplet sequences of peptides released by collagenase digestion of a collagen preparation from kidney cortex. Composition of the collagen preparation indicated that it was largely or wholly of basement membrane origin. 3-Hydroxyproline was detected in only one sequence, the tripeptide, glycyl-3-hydroxyprolyl-4-hydroxyproline, which accounted for a major fraction of the total 3-hydroxyproline obtained in the peptides released by collagenase. Preliminary data, based on sequencing the peptide mixture released by collagenase treatment, suggested that, in contrast, 4-hydroxyproline occurs predominantly if not exclusively in the Y position of Gly-X-Y triplet sequences in the collagen preparation studied.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001485:Basement Membrane / Q000032:analysis*; D003094:Collagen; D005971:Glutamates / Q000032:analysis; D005998:Glycine / Q000032:analysis; D006909:Hydroxyproline / Q000032:analysis*; D007672:Kidney Cortex / Q000032:analysis*; D003012:Microbial Collagenase; D009842:Oligopeptides / Q000032:analysis*; D012694:Serine / Q000032:analysis; D013552:Swine
D000596:Amino Acids; D005971:Glutamates; D009842:Oligopeptides; D012694:Serine; D003094:Collagen; D003012:Microbial Collagenase; D006909:Hydroxyproline; D005998:Glycine