[RNA fragments rich in G and A nucleotides obligatory for in vitro DNA replication of phages phi-X 174 and lambda].
Evidence was presented that in vitro conversion of single-stranded DNA of phage phi X 174 to the double-stranded replicative form by partially purified DNA-dependent DNA polymerase I requires a specific RNA fragment acting as primer (25-50 nucleotides). RNA fragments highly rich in nucleotides A and G were obtained by partial degradation of E. coli M 500 Sho-R ribosomal RNA with pancreatic ribonuclease. They become covalently bound to the newly synthesized DNA chain of the replicative form of phage phi X 174. These RNA fragments are also required for in vitro replication of lambda phage DNA.
D000249:Adenosine Monophosphate / Q000032:analysis; D003090:Coliphages / Q000378:metabolism*; D004261:DNA Replication; D006157:Guanosine Monophosphate / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis*; D014779:Virus Replication
D012367:RNA, Viral; D000249:Adenosine Monophosphate; D006157:Guanosine Monophosphate
ARN-amorceurs riches en nucléotides G et A indispensables à la réplication in vitro de l'ADN des phages phi X 174 et lambda