title: Adrenal Cortical Ribonuclease H (hybrid) (38494).
abstract: Ribonuclease Hybrid (RNaseH) from adrenal cortical tissue has been characterized. RNase H specifically degrades the RNA strand of purified RNA:DNA hybrids but is inactive on single- or double-stranded RNA or on DNA. The mode of clevage by RNase H is endonucleolytic, producing oligoribonucleoties with 3'-hydroxyl and 5'- phosphate termini. ACTH administration to guinea pigs results in no detectable change in adrenal cortical RNase H activity at times when changes in DNA synthesis are marked.
mesh_terms: D000302:Adrenal Cortex / Q000201:enzymology*; D000311:Adrenal Glands / Q000201:enzymology*; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D003600:Cytosol / Q000032:analysis; D004720:Endonucleases / Q000302:isolation & purification*; D006168:Guinea Pigs; D009693:Nucleic Acid Hybridization; D012313:RNA; D012260:Ribonucleases / Q000302:isolation & purification*; D013698:Templates, Genetic
chemical_list: D012313:RNA; D000324:Adrenocorticotropic Hormone; D004720:Endonucleases; D012260:Ribonucleases