[In vitro induction of numerical changes in the karyotype of normal and transformed Djungarian and Chinese hamster cells].
The comparative study of the frequency of colcemid-induced aneuploidy and polyploidy in cultured normal and transformed cells of Djungarian hamster is described. The occurrence of variants with changed chromosome number is much higher in populations of SV40-transformed cell line (4/21) than in normal embryonic cultures. In transformed lines of Djungarian and Chinese hamsters (4/21 and V-79) the frequency of cells with changed chromosome number was found to be dependent on the culture density: the percentage of polyploids was 4-5-fold higher when the number of seeded cells was 2-fold lower. The highest number (18-29%) of hypermodal cells was produced at drug concentrations of 0.02-0.025 mkg/ml. The percengate of polyploids under these conditions reached 10-20. At further increase of colcemid concentrations the proportion of polyploid cells increased. In Djungarian hamster embryonic cell cultures there were single cells with changed chromosome numbers at a concentration of the drug of 0.015-0.1 mkg/ml.
D000782:Aneuploidy; D000818:Animals; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000187:drug effects; D003078:Colchicine / Q000494:pharmacology*; D006224:Cricetinae; D008938:Mitosis / Q000187:drug effects; D011123:Polyploidy; D013539:Simian virus 40
D003078:Colchicine
Induktsiia in vitro chislovykh izmeneniĭ kariotipa v normal'nykh i transformirovannykh kletkakh dzhungarskogo i kitaiĭskogo khomiachkov