title: Amanitin content and toxicity of Amantia verna Bull.
abstract: The alpha-, beta-, and gamma-amanitin content of 11 samples of Amanita verna Bull., collected during 1975--1978 in Germany and Switzerland, has been determined by high performance thin-layer chromatography (HPTLC) of crude methanolic extracts. The toxicity (i. v. LD50 for mice of defatted, lyophilized, methanolic extracts) of 3 samples has been compared with that of A. phalloides from the same site of collection. The amanitin content of A. verna ranged from 2250 to 4570 mg/kg dry weight; the fungi contained almost as much beta- as alpha-amanitin, whereas the gamma-amanitin content amounted to about 12% of the total amanitin. A verna contained less amanitin (65% on the average) than A. phalloides from the same collection site, but it was not significantly less toxic, since the phallotoxins contributed to the toxicity of either species in our tests.
mesh_terms: D000363:Agaricales / Q000032:analysis*; D000545:Amanita / Q000032:analysis*; D000546:Amanitins / Q000032:analysis* / Q000633:toxicity; D000818:Animals; D005260:Female; D007928:Lethal Dose 50; D051379:Mice; D013045:Species Specificity
chemical_list: D000546:Amanitins