Fluorimetric determination of dipeptidyl carboxypeptidase. (angiotensin-I-converting enzyme).
A fluorimetric assay of dipeptidyl carboxypeptidase is described. It involves incubation at 37 degrees C with the substrate, Z-Phe-His-Leu, and reaction of the dipeptide His-Leu which is released upon enzymatic hydrolysis, with o-phthalaldehyde to yield a fluorescent compound. The method is simple, precise and sensitive. The assay in serum is conveniently performed on 20 mul samples. Normal values in human serum range from 0.04 to 0.22 U/l.
D001593:Benzyl Compounds; D004151:Dipeptides; D006801:Humans; D007700:Kinetics; D008722:Methods; D008832:Microchemistry; D007703:Peptidyl-Dipeptidase A / Q000097:blood*; D013050:Spectrometry, Fluorescence
D001593:Benzyl Compounds; D004151:Dipeptides; D007703:Peptidyl-Dipeptidase A