Heat stable cell growth inhibiting factor isolated from rat liver microsomes.
A heat stable cell growth inhibiting factor was isolated from rat liver microsomes by hot salt extraction, ethanol fractionation and the hot phenol method. The factor was contained in the RNA fraction (designated as mhRNA). mhRNA inhibited the growth of mouse fibroblast (L-929) cells at a relatively low concentration (55 microgram/ml of culture medium). The molecular weight of mhRNA was about 27,000 and the base composition was guanine and cytosine rich.
D000818:Animals; D002469:Cell Separation; D002478:Cells, Cultured; D003600:Cytosol / Q000032:analysis; D006131:Growth Inhibitors / Q000302:isolation & purification*; D006358:Hot Temperature; D008297:Male; D008862:Microsomes, Liver / Q000032:analysis*; D008970:Molecular Weight; D012313:RNA / Q000032:analysis; D051381:Rats
D006131:Growth Inhibitors; D012313:RNA