Concerning the subcellular distribution of 3beta-hydroxysteroid dehydrogenase/isomerase in the rat adrenal.
The distribution of 3beta-hydroxysteroid dehydrogenase/isomerase in the subcellular fractions of rat adrenal gland has been determined. Based on the total activity found, 55% was in the microsomal fraction, 10% in the heavy-mitochondrial fraction, 3% in the light-mitochondrial fraction and 26% in the fraction consisting of cell-debris plus nuclei. Ninety-five percent of the total activity was recovered in the fractions. Approximately half the activity in the heavy-mitochondrial fraction could be accounted for by microsomal contamination.
D000311:Adrenal Glands / Q000201:enzymology*; D000818:Animals; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000201:enzymology; D003576:Electron Transport Complex IV / Q000032:analysis; D049671:History, 18th Century; D006913:Hydroxysteroid Dehydrogenases / Q000032:analysis*; D008297:Male; D008861:Microsomes / Q000201:enzymology; D008928:Mitochondria / Q000201:enzymology; D011375:Progesterone Reductase / Q000032:analysis*; D051381:Rats; D013250:Steroid Hydroxylases / Q000032:analysis
D006913:Hydroxysteroid Dehydrogenases; D011375:Progesterone Reductase; D013250:Steroid Hydroxylases; D003576:Electron Transport Complex IV