title: The respiratory electron transport system of heterotrophically-grown Rhodopseudomonas palustris.
abstract: The enzymatic activities and the cytochrome components of the respiratory chain were investigated with membrane fractions from chemoheterotrophically growth Rhodopseudomonas palustris. Whereas the level of electron transfer carriers was not distinctly affected by a change of the culture conditions, the potential activities of the enzymes were clearly increased when the cells were grown aerobically. Reduced-minus oxidized difference spectra of the membrane fractions prepared from dark aerobically grown cells revealed the presence of three beta-types cytochromes b561, b560 and b558, and at least two c-type cytochromes c556 and c2 as electron carriers in the electron transfer chain. Cytochrome of a-type could not be detected in these membranes. Reduced plus CO minus reduced difference spectra of the membrane fractions were indicative of cytochrome o, which may be equivalent to cytochrome b560, appearing in substrate-reduced minus oxidized difference spectra. Cytochrome o was found to be the functional terminal oxidase. CO difference spectra of the high speed supernatant fraction indicated the presence of cytochrome c'. Succinate and NADH reduced the same types of cytochromes. However, a considerable amount of cytochrome b561 with associated beta and gamma bands at 531 and 429 nm, respectively, was reducible by succinate, but not by NADH. A substantial fraction of the membrane-bound b-type cytochrome was non-substrate reducible and was found in dithionite-reduced minus substrate-reduced spectra. Cytochrome c2 may be localized in a branch of the electron transport system, with the branch-point at the level of ubiquinone. The separate pathways rejoined at a common terminal oxidase. Two terminal oxidases with different KCN sensitivity were present in the respiratory chain, one of which was sensitive to low concentrations of KCN and was connected with the cytochrome chain. The other terminal oxidase which was inhibited only by high concentrations of cyanide was located in a branched pathway, through which the electrons could flow from ubiquinone to oxygen bypassing the cytochrome chain.
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D000968:Antimycin A / Q000494:pharmacology; D001205:Ascorbic Acid / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D003486:Cyanides / Q000494:pharmacology; D003580:Cytochromes / Q000378:metabolism; D003624:Darkness; D004579:Electron Transport / Q000187:drug effects; D006420:Hemeproteins / Q000378:metabolism; D008027:Light; D009243:NAD / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism; D010101:Oxygen Consumption; D010618:Phenanthrolines / Q000494:pharmacology; D012241:Rhodopseudomonas / Q000378:metabolism*; D013053:Spectrophotometry; D013386:Succinates / Q000378:metabolism; D013772:Tetramethylphenylenediamine / Q000378:metabolism; D014269:Trifluoroacetic Acid / Q000494:pharmacology; D014451:Ubiquinone / Q000378:metabolism
chemical_list: D003486:Cyanides; D003580:Cytochromes; D006420:Hemeproteins; D010618:Phenanthrolines; D013386:Succinates; D009243:NAD; D014451:Ubiquinone; D000968:Antimycin A; D014269:Trifluoroacetic Acid; D010088:Oxidoreductases; D013772:Tetramethylphenylenediamine; D001205:Ascorbic Acid