Establishment and characterization of an Epstein-Barr virus (EBC)-negative lymphoblastoid B cell line (BJA-B) from an exceptional, EBV-genome-negative African Burkitt's lymphoma.
An Epstein-Barr virus (EBV)-negative lymphoblastoid cell line (LCL), BJA-B, was established from an African Burkitt's lymphoma (BL) which contained no detectable EBV DNA and did not express the EBV specified antigen EBNA, BJA-B cells grow in typically large, flat clumps. All carry surface-bound immunoglobulins, a B lymphocyte marker, and do not form rosettes with sheep erythrocytes. After infection of BJA-B cells by EBV the infected cells may produce either EBV-determined nuclear antigen (EBNA) or both EBNA and early antigen (EA), depending on the strain of EBV. The homogeneity of the BJA-B cell population with respect to immunological and isoenzyme markers and size suggests a clonal origin of the line. BJA-B is the first EBV-negative LCL established from an African Burkitt's lymphoma and demonstrates that an EBV-independent continuous B cell line can be established "in vitro" from other than leukemia or myeloma cells.
D000956:Antigens, Viral / Q000032:analysis; D001402:B-Lymphocytes; D002051:Burkitt Lymphoma / Q000276:immunology / Q000382:microbiology*; D002460:Cell Line; D002675:Child, Preschool; D002875:Chromosomes; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis; D004854:Herpesvirus 4, Human / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D007128:Immunoglobulin Fragments / Q000032:analysis; D007527:Isoenzymes / Q000032:analysis; D008213:Lymphocyte Activation; D008855:Microscopy, Electron, Scanning; D010736:Phosphoglycerate Mutase / Q000032:analysis
D000956:Antigens, Viral; D007128:Immunoglobulin Fragments; D007527:Isoenzymes; D005954:Glucosephosphate Dehydrogenase; D010736:Phosphoglycerate Mutase