[Sequence complexity of transcribed unique DNA sequences in genome of mouse P815 mastocytoma cells (author's transl)].
The sequence complexity of nuclear RNA from mouse liver, mouse spleen and highly malignant P815 mastocytoma was measured by nRNA driven hybridization to unique DNA sequences of P815 cells. The unique DNA sequences represent 63% of the total nuclear DNA of P815 cells and their availibility in hybridization experiments was found to be 76%. Of these sequences 7.8% formed hybrids with nuclear RNA of this cell, about 11.5% with mouse spleen and about 14.5% with mouse liver nuclear RNA. Assuming an asymmetrical transcription, the complexities of these transcripts are 2.8 X 10(8) nucleotides for mouse P815 mastocytomas, 4.3 X 10(8) for mouse spleen and about 5.3 X 10(8) nucleotides for mouse liver. Cellular specifity of the transcribed information was analyzed in additivity experiments, in which unique DNA sequences, not complementary to the nuclear RNA of one cell were annealed to the nuclear RNAs of the two other tissues/cells. In these experiments most of the nuclear RNA sequences of P815 cells were found to be also present in the nucleus of mouse liver and spleen. Only a small portion of the unique DNA sequences of P815 mastocytoma (about 1.2% corresponding to 4.4 X 10(7) nucleotides) was found to be complementary only to P815 mastocytoma nuclear RNA.
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D007700:Kinetics; D008099:Liver / Q000032:analysis*; D012515:Mast-Cell Sarcoma / Q000032:analysis*; D051379:Mice; D009693:Nucleic Acid Hybridization; D012332:RNA, Heterogeneous Nuclear / Q000302:isolation & purification; D013154:Spleen / Q000032:analysis*
D012332:RNA, Heterogeneous Nuclear
Die Sequenzkomplexität transkribierter unique DNA-Sequenzen im Genom von Maus P815 Mastocytoma Zellen.