Compositional characteristics of a chloroform/methanol soluble protein fraction from spinach chloroplast membranes.
Extraction of an aqueous suspension of spinach chloroplast lamellae with a chloroform/methanol mixture leads to solubilization of about 1/3 of the total membrane protein. Amino acid analysis of the chloroform/methanol-soluble protein shows that this fraction is largely enriched in the hydrophobic residues proline, leucine, alanine and phenylalanine and considerably depleted in polar amino acids, namely lysine and arginine. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the solubilized material reveals the presence of a variety of low molecular weight polypeptides (molecular weight less than or equal to 25 000), with more than 50% of the total fraction being contributed by a 25 000 dalton band. This band, which accounts for about 25% of the total chloroplast lamellar protein, has recently been identified as the main component of the light-harvesting chlorophyll-protein complex. The physiological role of most of the chloroform/methanol-soluble protein fraction is not known at present. From its chemical properties and apparent biological inertness, we propose that it plays mainly a structural role in situ, interacting with the lipid moiety of the chloroplast membrane. The material insoluble in the aqueous chloroform/methanol mixture is largely enriched in manganese, iron, cytochrome and water-soluble proteins, such as chloroplast coupling factor and ribulose diphosphate carboxylase.
D000596:Amino Acids / Q000032:analysis; D001665:Binding Sites; D002462:Cell Membrane / Q000378:metabolism; D002725:Chloroform; D002736:Chloroplasts / Q000378:metabolism*; D003574:Cytochrome c Group / Q000032:analysis; D007501:Iron / Q000032:analysis; D008345:Manganese / Q000032:analysis; D000432:Methanol; D008970:Molecular Weight; D010940:Plant Proteins / Q000378:metabolism; D010944:Plants / Q000378:metabolism; D011485:Protein Binding; D012995:Solubility
D000596:Amino Acids; D003574:Cytochrome c Group; D010940:Plant Proteins; D008345:Manganese; D002725:Chloroform; D007501:Iron; D000432:Methanol