The hydrosmotic effect of vasopressin: a scanning electrom-microscope study.
Scanning electron-microscopy (SEM) was used to investigate the hydrosmotic effect of vasopressin on the apical surface of urinary bladders of toads Bufo marinus. Bladders were mounted on glass chambers and water fluxes were monitored with an optical method. Tissues were fixed in 2% glutaraldehyde and processed for SEM. Three types of cells were seen on the surface of control bladders:large polygonal (granular) cells, with blunt microvilli; smaller (mitochondria-rich) cells, with longer microvilli; goblet cells. Neither exposure of the bladders to a large osmotic gradient nor exposure to vasopressin in the absence of a gradient altered appreciably the epithelial surface. In contrast, the combination of vasopressin and an osmotic gradient resulted ina conspicuous diminution of the blunt microvilli. However, the small cells with longer microvilli remained unchanged. Identical results were seen with cAMP or theophylline in the presence of an osmotic gradient. These findings suggest that the hydrosmotic effect of vasopressin is mainly exerted on the granular cells of toad bladder and confirm observations made by others with the electron-microscope.
D000818:Animals; D001692:Biological Transport; D002024:Bufo marinus; D002463:Cell Membrane Permeability / Q000187:drug effects; D000242:Cyclic AMP / Q000494:pharmacology; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure; D008855:Microscopy, Electron, Scanning; D009997:Osmotic Pressure; D013806:Theophylline / Q000494:pharmacology; D001743:Urinary Bladder / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure*; D014667:Vasopressins / Q000494:pharmacology*; D014867:Water / Q000378:metabolism
D014867:Water; D014667:Vasopressins; D013806:Theophylline; D000242:Cyclic AMP