Primary tissue culture of human wart-derived epidermal cells (keratinocytes).
The formation of human skin warts may depend on transformation by human papilloma virus or result from a productive virus infection. To explore this problem, methods were developed for the primary tissue culture of epidermal cells (keratinocytes) derived from wart tissues and normal skin. Wart-derived keratinocytes continued to proliferate for periods exceeding 3 months, but the patterns of cell growth were identical to normal keratinocytes. Electron microscopic and immunohistologic examination of wart-derived cultures did not evidence virion assembly.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D001706:Biopsy; D002455:Cell Division; D046508:Culture Techniques; D006801:Humans; D027383:Papillomaviridae / Q000276:immunology / Q000302:isolation & purification; D011817:Rabbits / Q000276:immunology; D014779:Virus Replication; D014860:Warts / Q000276:immunology / Q000382:microbiology / Q000473:pathology*
D000956:Antigens, Viral