Electron microscopy of vesicular-arbuscular mycorrhizae of yellow poplar. II. Intracellular hyphae and vesicles.
Intracellular hyphae and vesicles in mycorrhizal roots of yellow poplar were examined by electron microscopy. An investing layer of host wall material and cytoplasm enclosed the endophyte within the cells. Young developing hyphae contained abundant cytoplasm and few vacuoles. As hyphae matured, they became highly vacuolated and accumulated carbohydrate (glycogen) and lipid reserves. Mature vesicles were engorged with lipid droplets, possessed a trilaminate wall and were also enclosed by host wall material and cytoplasm. Compared with uninfected cells, infected cortical cells showed an increase in cytoplasmic volume, enlarged nuclei, and a reduction of starch reserves. Host nuclei were always proximal to the hyphae during hyphal development and deterioration. While other cytoplasmic components of infected and uninfected cells were comparable large electron-dense bodies occurred in vacuoles of most cells containing hyphae. Deterioration of intracellular hyphae occurred throughout the samples examined. Septa separated functional and degenerating portions of the hyphae. Hyphal deterioration involved degeneration and ultimate disappearance of fungal cytoplasm as well as collapse of hyphal walls. Based on these observations, the authors hypothesize that deterioration of the endophyte may release significant quantities of mineral nutrients, via hyphal contents, which are absorbed by the host.
D002473:Cell Wall / Q000648:ultrastructure; D003593:Cytoplasm / Q000382:microbiology / Q000648:ultrastructure; D005658:Fungi / Q000648:ultrastructure*; D006003:Glycogen; D002479:Inclusion Bodies / Q000648:ultrastructure; D008055:Lipids; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D009090:Mucorales / Q000254:growth & development / Q000648:ultrastructure*; D010944:Plants / Q000382:microbiology*; D012988:Soil Microbiology; D013559:Symbiosis; D014197:Trees
D008055:Lipids; D006003:Glycogen