Enzymic activity of purified plasma membranes from the yeast and mycelial forms of Candida albicans.
Plasma membranes were isolated from the yeast and mycelial forms of Candida albicans as described previously (Marriott, 1975) and examined for the presence of several enzymes. Measurement of specific activities showed enrichment of Mg2+-dependent and Ma+/K+-stimulated Mg2+-dependent adenosine triphosphatase and mannan synthetase, in the plasma membrane fractions from both morphological forms of the organism. However, acid and alkaline phosphatase, NADH oxidase and 5'-nucleotidase showed no such specific location.
D000135:Acid Phosphatase / Q000032:analysis; D000251:Adenosine Triphosphatases / Q000032:analysis; D000469:Alkaline Phosphatase / Q000032:analysis; D002176:Candida albicans / Q000201:enzymology* / Q000254:growth & development; D002458:Cell Fractionation; D002462:Cell Membrane / Q000201:enzymology; D006150:Guanine Nucleotides; D006602:Hexosyltransferases / Q000032:analysis*; D008358:Mannose; D008364:Mannosyltransferases; D009244:NAD+ Nucleosidase / Q000032:analysis; D009247:NADH, NADPH Oxidoreductases / Q000032:analysis*; D010042:Ouabain / Q000494:pharmacology; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis*; D011523:Protoplasts / Q000201:enzymology; D013347:Subcellular Fractions / Q000201:enzymology
D006150:Guanine Nucleotides; D010042:Ouabain; D009247:NADH, NADPH Oxidoreductases; D006602:Hexosyltransferases; D008364:Mannosyltransferases; C021542:mannan synthetase; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D010744:Phosphoric Monoester Hydrolases; D009244:NAD+ Nucleosidase; D000251:Adenosine Triphosphatases; D008358:Mannose