title: Pathogenic and serologic studies of Japanese quail infected with JM strain of Marek's disease herpesvirus.
abstract: Japanese quail were inoculated with Marek's disease herpesvirus at 1 day of age, and a second group of the same age was infected by contact with inoculated quail. A third group raised in our laboratory as a source of hatching eggs was accidentally infected with Marek's disease (MD). All quail were observed and examined for periods up to 360 days; the usual clinical signs of MD were not observed. However, the birds were susceptible to MD infection because of the evidence that: 1) The MD-specific agar gel-preceipitation (AGP) antigen was found in the feather tips or feather follicular epithelium of some of them; 2) the MD-specific AGP antibody was found in 6 of 57 exposed to MD; and 3) the virus was recovered from 4 MD-exposed birds by subpassage of their kidney cultures into quail embryo fibroblasts. The specificity of the AGP antigen or antibody in MD-infected quail was examined by the line of identity and cross-absorption tests. The antigen specificity was tested against similar to 700 sera from chickens and quail. Although most of these sera reacted with quail-positive antigen (QPA) prepared from MD-infected quail, some sera from infected or uninfected normal chickens reacted nonspecifically with QPA and with an antigen prepared from normal quail. By gel filtration on Sephadex G-150, the specific antigenic component could be separated from the nonspecific component(s) in QPA.
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D000918:Antibody Specificity; D000956:Antigens, Viral / Q000032:analysis*; D002850:Chromatography, Gel; D003370:Coturnix; D005241:Feathers / Q000276:immunology; D005347:Fibroblasts / Q000382:microbiology; D008381:Herpesvirus 2, Gallid / Q000302:isolation & purification / Q000472:pathogenicity*; D007668:Kidney / Q000382:microbiology; D008380:Marek Disease / Q000276:immunology*; D011201:Poultry Diseases / Q000276:immunology*; D011233:Precipitin Tests
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral