[Protective effect of thymidine in relation to the cytostatic action of cyclic adenosine-3', 5'-monophosphate in mammalian cell cultures].
Under the action of lmM of cyclic adenosine-3',5'-monophosphate (cAMP) the survival and the rate of reproduction of the cells of Chinese hamster in the culture decreased in comparison with control to 27 and 42%, respectively. Addition of 0.02 mM thymidine together with cAMP almost completely eliminated the cytostatic action of the latter. Thymidine also prevented the cytostatic effect of noncyclic 5'-AMR, but failed to influence the death of the cells with the action of dibutyril cAMP and theophylline. Thymidine failed to prevent the inhibitory action of cAMP on the mutant strains of mouse cells defective by thymidinekinase. A conclusion was drawn that in the concentrations under study thecytostatic action of the exogenous cAMP on the cells of mammals served as the sequence of splitting to 5'-AMP in the culture medium and was realized by blocking one of the stages of TMP synthesis.
D003994:Bucladesine / Q000037:antagonists & inhibitors; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured / Q000187:drug effects / Q000201:enzymology; D000242:Cyclic AMP / Q000037:antagonists & inhibitors*; D013806:Theophylline / Q000037:antagonists & inhibitors; D013936:Thymidine / Q000494:pharmacology*; D013937:Thymidine Kinase / Q000378:metabolism
D003994:Bucladesine; D013806:Theophylline; D000242:Cyclic AMP; D013937:Thymidine Kinase; D013936:Thymidine
Zashchitnoe vliianie timidina v otnoshenii tsitostaticheskogo deĭstviia tsiklicheskogo adenozin-3', 5'-monofosfata v kul'ture kletok mlekopitaiushchikh