Localization of enzymes in specialized regions of the microsomal membrane.
Microsomal vesicles were centrifuged through sucrose density gradients containing deoxycholate. With 0.15% detergent electron transport enzymes and phosphatases could be separated. Increasing the deoxycholate concentration to 0.19% resulted in separation of the microsomal material into five bands containing (in order from the top of the gradient) adenosine monophosphatase, inosine diphosphatase and some glucose-6-phosphatase (band 1); NADH-linked (band 2) and NADH-linked (band 3) electron transport enzymes; and glucose-6-phosphatase (bands 4 and 5). It appears that enzymes are arranged in specialized patches in the microsomal membrane.
D000818:Animals; D002458:Cell Fractionation; D002499:Centrifugation, Density Gradient; D003579:Cytochrome Reductases / Q000378:metabolism; D003840:Deoxycholic Acid; D004579:Electron Transport; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D008297:Male; D008566:Membranes / Q000201:enzymology*; D008862:Microsomes, Liver / Q000201:enzymology*; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D051381:Rats
D003840:Deoxycholic Acid; D003579:Cytochrome Reductases; D010744:Phosphoric Monoester Hydrolases; D005952:Glucose-6-Phosphatase; D010727:Phosphoric Diester Hydrolases