title: Identification and distribution of swine serum immunoglobins that react with Trichinella spiralis antigens and may interfere with the enzyme-labeled antibody test for trichinosis.
abstract: Sera from Trichinella spiralis digestion-negative swine contained variable amounts of two immunoglobins that reacted with T spiralis antigen in the indirect enzyme-labeled antibody test for trichinosis. One of these immunoglobins, detected by heavy chain-specific anti-swine immunoglobulin G (IgG) conjugate, was removed by absorption with T spiralis larvae. A second immunoglobin, detected by heavy chain-specific anti-swine IgM, was not removed by absorption with T spiralis larvae and increased in amount with the age of the animal. These two immunoglobins varied independently in individual animals and showed some specificity for the antigen; ie, they did not merely reflect changes in total serum IgG or IgM. In contrast to IgG anti-T spiralis antibody from experimentally infected animals, neither of these immunoglobins could be detected in double-diffusion tests against the antigen or by counter immunoelectrophoresis. Either of these immunoglobins could interfere with the indirect test for T spiralis antibodies, depending upon whether anti-swine IgG or IgM conjugate is used. The factors which initiate synthesis and control serum concentrations of these immunoglobins are not known.
mesh_terms: D000818:Animals; D000937:Antigen-Antibody Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005188:False Negative Reactions; D005189:False Positive Reactions; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007136:Immunoglobulins / Q000032:analysis*; D013047:Specific Pathogen-Free Organisms; D013552:Swine / Q000276:immunology*; D013553:Swine Diseases / Q000175:diagnosis* / Q000276:immunology; D014234:Trichinella / Q000276:immunology*; D014235:Trichinellosis / Q000276:immunology / Q000662:veterinary*
chemical_list: D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins