Degradation of phospholipid in Pseudomonas aeruginosa induced by polymyxin B.
Effects of polymyxin B on the synthesis and degradation of lipid, ribonucleic acid (RNA) and protein in Pseudomonas aeruginosa were investigated. It was found that polymyxin B caused a marked degradation of the lipid fraction which was prelabeled with (3H-2)-glycerol. Thin-layer chromatographic analysis indicated that the main degraded lipids were phosphatidylethanolamine and phosphatidylglycerol, which constituted 80% and 15% of the total phospholipids of this organism, respectively. Polymyxin B also inhibited synthesis of RNA and protein in vivo. The severe inhibition of the uptake of labeled amino acids by polymyxin B indicated that the observed inhibition of RNA and protein synthesis possibly occurred at the level of substrate transports. The degradation of phospholipid might account for the defective membrane activities.
D001426:Bacterial Proteins / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D050356:Lipid Metabolism; D010649:Phenylalanine / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000378:metabolism; D010715:Phosphatidylglycerols / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*; D011113:Polymyxins / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000254:growth & development / Q000378:metabolism*; D012329:RNA, Bacterial / Q000378:metabolism; D013997:Time Factors; D014498:Uracil / Q000378:metabolism
D001426:Bacterial Proteins; D010714:Phosphatidylethanolamines; D010715:Phosphatidylglycerols; D010743:Phospholipids; D011113:Polymyxins; D012329:RNA, Bacterial; D010649:Phenylalanine; D014498:Uracil; D005990:Glycerol