Alterations in lipid acyl group composition and membrane structure in cells transformed by Rous sarcoma virus.
The acyl group composition of the phospholipids from normal chick embryo fibroblasts and from cells transformed by Rous sarcoma virus was determined by gas-liquid chromatography. Rous-transformed cells had less arachidonate (20:4) and more oleate (18:1) in membrane lipids than normal, growing cells. Normal density-inhibited cells had the lowest ratio of 18:1/20:4. Associated with the decreased content of 20:4 in the transformed cells was a decreased motional freedom of an incorporated spin-labeled fatty acid analogue. Arrhenius plots for uptake of 2-deoxyglucose revealed an increased apparent activation energy in the transformed cells, suggesting that the hexose transport carriers were sensitive to the changes in membrane composition and structure in fully transformed cells. However, the development of the changes in fatty acid composition occurred relatively slowly in the course of transformation, indicating that the observed compositional alterations are not likely to be a primary cause of the early changes in membrane function associated with malignant transformation.
D000818:Animals; D001095:Arachidonic Acids / Q000378:metabolism; D001358:Avian Sarcoma Viruses; D002462:Cell Membrane / Q000378:metabolism*; D002471:Cell Transformation, Neoplastic; D002642:Chick Embryo; D003847:Deoxyglucose / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D005227:Fatty Acids / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism*; D007700:Kinetics; D009829:Oleic Acids / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism*; D013229:Stearic Acids; D013696:Temperature
D001095:Arachidonic Acids; D005227:Fatty Acids; D009829:Oleic Acids; D010743:Phospholipids; D013229:Stearic Acids; D003847:Deoxyglucose