title: Arthrobacter luteus restriction endonuclease recognition sequence and its cleavage map of SV40 DNA.
abstract: The nucleotide sequence at the cleavage site of the restriction endonuclease isolated from Arthrobacter luteus (Alu) has been determined. The endonuclease cleaves at the center of a palindromic tetranucleotide sequence to give even-ended duplex DNA fragments phosphorylated at the 5'-end. The endonuclease cleaves SV40 form I DNA into 32 fragments. The order and sizes of these fragments have been determined to provide an Alu cleavage map of the SV40 genome.
mesh_terms: D001173:Arthrobacter / Q000201:enzymology*; D001483:Base Sequence; D004279:DNA, Viral; D003851:Deoxyribonucleases / Q000378:metabolism*; D003854:Deoxyribonucleotides / Q000032:analysis; D006193:Haemophilus influenzae / Q000201:enzymology; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000032:analysis; D013539:Simian virus 40; D013045:Species Specificity
chemical_list: D004279:DNA, Viral; D003854:Deoxyribonucleotides; D009838:Oligodeoxyribonucleotides; D003851:Deoxyribonucleases