A ribosome-dependent GTPase from yeast distinct from elongation factor 2.
Three proteins required for poly(U)-directed polyphenylalanine synthesis have been separated from yeast. Two of the factors correspond to the elongation factors 1 and 2 described for other eukaryotic systems, according to the criteria of phenylalanyl-tRNA binding and diphtheria toxin-catalyzed ADP-ribosylation. The third protein, while absolutely required for polyphenylalanine synthesis, was a more active ribosome-dependent GTPase than elongation factor 2.
D000244:Adenosine Diphosphate / Q000378:metabolism; D002474:Cell-Free System; D004167:Diphtheria Toxin / Q000378:metabolism; D005672:Fusidic Acid / Q000494:pharmacology; D020963:GTP Phosphohydrolase-Linked Elongation Factors / Q000037:antagonists & inhibitors / Q000378:metabolism*; D010441:Peptide Chain Elongation, Translational; D010445:Peptide Elongation Factors / Q000302:isolation & purification*; D010649:Phenylalanine / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D011072:Poly U / Q000378:metabolism; D012343:RNA, Transfer / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
D004167:Diphtheria Toxin; D010445:Peptide Elongation Factors; D011072:Poly U; D010649:Phenylalanine; D005672:Fusidic Acid; D000244:Adenosine Diphosphate; D012343:RNA, Transfer; D010744:Phosphoric Monoester Hydrolases; D020963:GTP Phosphohydrolase-Linked Elongation Factors