Studies of Epstein-Barr virus (ebv)-associated nuclear antigen. I. assay in human lymphoblastoid cell lines by direct and indirect determination of 125I-IgG binding.
A quantitative assay for EBNA in human lymphoblastiod cell lines has been developed. The assay employs EBNA-positive and -negative 125-I-IgG preparations as reagents and can be used in a direct or indirect manner. EBNA specificity has been demonstrated in a number of ways. The antigenic relationship between EBNA  present in Raji cells and in a number of other human lymphoblastiod cell lines of diverse origins and between the cell-associated antigen present in FT Raji cells and that present in isolated Raji nuclei has been studied. The possibility of carrying out blocking titrations for anti-EBNA determination has been demonstrated and the effect of glutaraldehyde and formaldehyde upon antigenic activity in FT Raji cells has been studied.
D000956:Antigens, Viral / Q000302:isolation & purification*; D002460:Cell Line; D002467:Cell Nucleus / Q000276:immunology; D003080:Cold Temperature; D004854:Herpesvirus 4, Human / Q000276:immunology*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007074:Immunoglobulin G / Q000032:analysis*; D007457:Iodine Radioisotopes; D011485:Protein Binding
D000956:Antigens, Viral; D007074:Immunoglobulin G; D007457:Iodine Radioisotopes