Carbon monoxide as a degradation product of cytochrome C and of ascorbic acid.
Cytochrome c has been incubated with 4 different reducing agents at 37 degrees C for 24 h at pH 7.0, and the spectral changes and the production of carbon monoxide have been followed. Only with hydrazine hydrate cytochrome c produced some carbon monoxide. Accidentally, carbon monoxide formation from ascorbic acid was found, amounting to about 0.1 mumol from 15 mumol of ascorbic acid in 24 h under the above conditions.
D000085:Acetates; D001205:Ascorbic Acid / Q000378:metabolism*; D002248:Carbon Monoxide / Q000378:metabolism*; D003574:Cytochrome c Group / Q000378:metabolism*; D006834:Hydrazines; D066298:In Vitro Techniques; D010084:Oxidation-Reduction; D013864:Thioglycolates
D000085:Acetates; D003574:Cytochrome c Group; D006834:Hydrazines; D013864:Thioglycolates; D002248:Carbon Monoxide; D001205:Ascorbic Acid