Differential effect of phosphonoacetic acid on the expression of Epstein-Barr viral antigens and virus production.
The effects of phosphonoacetic acid on cell growth, expression of Epstein-Barr virus antigens, and virus production in human and marmoset lymphoblastoid cell lines have been studied. The drug had no significant effect at concentrations up to 100 mug/ml on cell growth or total cell DNA synthesis. Higher doses induced not only a drastic decrease in DNA synthesis and cell grwoth, but also a dramatic cell enlargement. Immunofluorescence studies showed that greater than or equal to 30 mug/ml of phosphonoacetic acid inhibited viral capsid antigen synthesis without affecting the expression of the nuclear antigen or the spontaneous and 5-iodo-2'-deoxyuridine-induced early antigens. Production of transforming Epstein-Barr virus was also blocked.
D000085:Acetates / Q000494:pharmacology*; D000956:Antigens, Viral; D000998:Antiviral Agents / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D004247:DNA / Q000096:biosynthesis; D004279:DNA, Viral / Q000096:biosynthesis; D004854:Herpesvirus 4, Human / Q000254:growth & development* / Q000276:immunology; D009943:Organophosphorus Compounds / Q000494:pharmacology*; D014764:Viral Proteins / Q000096:biosynthesis; D014779:Virus Replication / Q000187:drug effects*
D000085:Acetates; D000956:Antigens, Viral; D000998:Antiviral Agents; D004279:DNA, Viral; D009943:Organophosphorus Compounds; D014764:Viral Proteins; D004247:DNA