Formation of nucleoprotein complexes between polyoma empty capsides and DNA.
Purified polyoma empty capsids and polyoma type I DNA interact in a cell-free system to form nucleoprotein complexes. Complexes that consist of one, two, three, and four empty capsids per DNA molecule have been detected. Polyoma virions or capsomers do not react with added DNA to form such complexes.
D001120:Arginine; D002250:Carbon Radioisotopes; D002474:Cell-Free System; D002499:Centrifugation, Density Gradient; D004279:DNA, Viral; D003851:Deoxyribonucleases; D004492:Edetic Acid; D004591:Electrophoresis, Polyacrylamide Gel; D008623:Mercaptoethanol; D008854:Microscopy, Electron; D009698:Nucleoproteins; D011120:Polyomavirus; D011402:Pronase; D012260:Ribonucleases; D012965:Sodium Chloride; D012967:Sodium Dodecyl Sulfate; D014316:Tritium; D014364:Tryptophan; D014764:Viral Proteins
D002250:Carbon Radioisotopes; D004279:DNA, Viral; D009698:Nucleoproteins; D014764:Viral Proteins; D014316:Tritium; D012967:Sodium Dodecyl Sulfate; D012965:Sodium Chloride; D008623:Mercaptoethanol; D014364:Tryptophan; D001120:Arginine; D004492:Edetic Acid; D003851:Deoxyribonucleases; D012260:Ribonucleases; D011402:Pronase