title: In-vitro cultivation of human cytomegalovirus in thyroid epithelial cells.
abstract: Several strains of human cytomegalovirus including recent isolates, were grown in epithelial cells derived from thyroid tissue. All the strains tested grew in these cultures without pre-treatment of the cells, and no difference in cytopathic effect was detected between strains of genital and non-genital origin. 4 strains of CMV were isolated directly from urine in thyroid cells; however the failure to isolate 6 further strains in these cultures from other specimens may indicate that a higher multiplicity of infection is required to infect epithelial than fibroblast cells.
mesh_terms: D002584:Cervix Uteri / Q000382:microbiology; D046508:Culture Techniques; D003587:Cytomegalovirus / Q000254:growth & development* / Q000302:isolation & purification; D003588:Cytopathogenic Effect, Viral; D005260:Female; D006801:Humans; D013961:Thyroid Gland / Q000382:microbiology*; D014556:Urine / Q000382:microbiology; D014776:Virus Cultivation / Q000379:methods*; D014779:Virus Replication