[Use of polyethylene glycol (PEG) in the isolation of the IBR virus].
Experiments were carried out to use polyethylene-glycol (PEG)--6000 in the isolation of the IBR virus from field material. The sedimentation with PEG proved easy, fast and selective. Large amounts of field material suspension could be treated which made it possible to attain volume concentration of such suspensions and to raise the relative concentration of the virus in them. Thus the virus could be isolated more quickly and in a more dependable way, avoiding the toxic effect of some of the initial materials on the cell culture.
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D007242:Herpesvirus 1, Bovine / Q000302:isolation & purification*; D007241:Infectious Bovine Rhinotracheitis / Q000382:microbiology; D008168:Lung / Q000382:microbiology; D008198:Lymph Nodes / Q000382:microbiology; D009297:Nasal Mucosa / Q000378:metabolism; D011092:Polyethylene Glycols; D014132:Trachea / Q000382:microbiology; D014776:Virus Cultivation
D011092:Polyethylene Glycols
Izpolzvane na polietilenglikol (PEG) pri izolirane na IBR-virus