[Effect of the method of cell disintegration on the aspartate kinase activity of preparations from Bacillus polymyxa var. Ross].
The influence of methods of cell disintegration of Bac. polymyxa on aspartate kinase activity (EC 2.7.2.4) was examined. The disruption by means of the Hews press yielded a more active preparation as compared with ultrasonic disintegration. The supernatant treatment with streptomycin sulphate increased the preparation activity 2-fold. Dialysis of the fraction with a high activity of aspartate kinase inactivated the enzyme by 80--85%. The relationship between the aspartate kinase activity and the portein and ATP concentration in the reaction mixture was established.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000332:Aerobiosis; D001222:Aspartate Kinase / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D001407:Bacillus / Q000201:enzymology*; D002458:Cell Fractionation / Q000379:methods*; D003470:Culture Media; D003956:Dialysis; D004789:Enzyme Activation / Q000187:drug effects; D004794:Enzyme Repression / Q000187:drug effects; D010770:Phosphotransferases / Q000378:metabolism*; D013307:Streptomycin / Q000494:pharmacology; D014461:Ultracentrifugation; D014465:Ultrasonics
D003470:Culture Media; D000255:Adenosine Triphosphate; D010770:Phosphotransferases; D001222:Aspartate Kinase; D013307:Streptomycin
Vliianie sposoba dezintegratsii kletok na aspartatkinaznuiu aktivnost' preparatov iz Bacillus polymyxa var. Ross