title: Mosquito cells infected with vesicular stomatitis virus yield unsialylated virions of low infectivity.
abstract: Vesicular stomatitis virus propagated in and released from Aedes albopictus cells had the normal complement of viral proteins; the glycoprotein contained carbohydrate but no sialic acid. These virions had markedly reduced hemagglutinating activity and exhibited a very high ratio of physical particles to infectious virus. In vitro sialylation of vesicular stomatitis virions grown in mosquito cells resulted in a 100-fold increase in both infectivity and hemagglutination titers to levels approaching those of virus grown in BHK-21 cells. These experiments provide an example of host-controlled modification of viral infectivity.
mesh_terms: D000330:Aedes / Q000382:microbiology*; D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D005944:Glucosamine; D006023:Glycoproteins / Q000032:analysis; D006387:Hemagglutination, Viral; D007668:Kidney; D012794:Sialic Acids / Q000032:analysis; D014316:Tritium; D014721:Vesicular stomatitis Indiana virus / Q000032:analysis / Q000254:growth & development*; D014764:Viral Proteins / Q000032:analysis; D014779:Virus Replication
chemical_list: D006023:Glycoproteins; D012794:Sialic Acids; D014764:Viral Proteins; D014316:Tritium; D005944:Glucosamine