Effect of hydroxycobalamin on the inhibition of cytochrome c oxidase by cyanide. II - In isolated cytochrome C oxidase.
The effects of hydroxycobalamin on inhibition of the isolated cytochrome c oxidase (EC 1.9.3.1) by cyanide were studied. No effect of hydroxycobalamin (HCo) and cyanocobalamin (CNCo) was observed on isolated cytochrome c oxidase. When cytochrome c oxidase inhibited by cyanide was treated with HCo the reversal of inhibition could be observed through the spectrum of cytochrome c oxidase without reversal of activity; CNCo had no effect. The treatment of cyano-cytochrome c oxidase with HCo suggests that there is the formation of cobalamin-cytochrome c oxidase complex plus CNCo, and it is possible that complex formation would be between the cobalt of cobalamin and proteic part of cytochrome c oxidase.
D000818:Animals; D002417:Cattle; D003486:Cyanides / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D003574:Cytochrome c Group / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000037:antagonists & inhibitors*; D066298:In Vitro Techniques; D009206:Myocardium / Q000201:enzymology; D010101:Oxygen Consumption / Q000187:drug effects; D014805:Vitamin B 12 / Q000494:pharmacology*
D003486:Cyanides; D003574:Cytochrome c Group; D003576:Electron Transport Complex IV; D014805:Vitamin B 12