Serum prolactin levels and prolactin binding activity in adrenals and kidneys of male rats after dehydration, salt loading, and unilateral nephrectomy.
Serum prolactin (PRL) levels and PRL binding activity in microsomal membranes from kidneys and adrenals were measured in control, water-deprived, unilaterally nephrectomized, and salt-loaded male rats. Unilateral nephrectomy and water deprivation increased serum prolactin levels significantly. Unilateral nephrectomy did not alter PRL binding activity in the kidneys, but significantly increased it in the adrenal glands. Salt loading had no effect on serum prolactin levels or PRL binding in the kidneys; but significantly increased PRL binding in the adrenal glands. Inhibition curves and tests of cross reactivity with LH, FSH, TSH, and GH showed that binding of PRL to its receptors in the kidneys and adrenals was specific. These observations suggest that PRL has a role in salt and water metabolism and that PRL receptors in the kidney and adrenals participate in this regulatory system.
D000311:Adrenal Glands / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D002458:Cell Fractionation; D003681:Dehydration / Q000378:metabolism; D005640:Follicle Stimulating Hormone / Q000378:metabolism; D013006:Growth Hormone / Q000378:metabolism; D007457:Iodine Radioisotopes; D007668:Kidney / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000378:metabolism; D008297:Male; D008861:Microsomes / Q000378:metabolism; D009392:Nephrectomy; D010544:Peroxidases; D011388:Prolactin / Q000097:blood* / Q000378:metabolism; D051381:Rats; D011956:Receptors, Cell Surface; D012965:Sodium Chloride / Q000378:metabolism; D013972:Thyrotropin / Q000378:metabolism; D014882:Water-Electrolyte Balance
D007457:Iodine Radioisotopes; D011956:Receptors, Cell Surface; D012965:Sodium Chloride; D011388:Prolactin; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D013972:Thyrotropin; D013006:Growth Hormone; D010544:Peroxidases