Induction of suppressor activity in the autologous mixed lymphocyte reaction and in cultures with concanavalin A.
T lymphocytes that are activated in the autologous mixed lymphocyte reaction (MLR) have suppressor activity. Concanavalin A (Con A) augments the suppressor activity generated in cultures containing both T and non-T lymphocytes and can induce suppressor activity in T-lymphocyte preparations that contain too few (10%) non-T cells to generate a significant autologous MLR. However, when such T-lymphocyte preparations are further depleted of adherent cells and contain less than 2% non-T cells, Con A fails to induce suppressor activity. These findings support the concept that an autologous MLR may play an important role in generation of suppressor cells by Con A.
D000917:Antibody Formation; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology*; D006801:Humans; D007108:Immune Tolerance; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D011839:Radiation, Ionizing; D013601:T-Lymphocytes / Q000276:immunology* / Q000528:radiation effects; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D003208:Concanavalin A