title: Porcine parvovirus: frequency of naturally occurring transplacental infection and viral contamination of fetal porcine kidney cell cultures.
abstract: The frequency of naturally occurring transplacental infection of swine with porcine parvovirus (PPV) and one of the possible consequences of such infection--the presence of PPV in cell cultures prepared from fetal tissues--were investigated. Transplacental infection was indicated by the presence of high titers of hemagglutination inhibiting (HI) antibody for PPV in serums of 0-day-old, hysterectomy-derived, colostrum-deprived pigs of 3 of 82 litters. All letters were farm-raised dams. Moreover, cell cultures prepared from 3 of 49 lots of fetal porcine kidneys (FPK) collected from an abattoir during an interval of 14 months were found contaminated with PPV. Because each lot was usually comprised of kidneys from 2 litters, the latter finding suggests that 3 of approximately 98 litters were infected. Prior infection of FPK cell cultures with PPV resulted in only slight interference of replication of other selected viruses; i.e., porcine enterovirus (PEV), pseudorabies virus (PRV), vesicular stomatitis virus (VSV), and hemagglutinating encephalomyelitis virus (HEV). Moreover, PPV and HEV were propagated in the same cell cultures during 5 serial passages of the viruses. In contrast, when copropagation of PPV and VSV was attempted, PPV was not detected after the 2nd serial passage.
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003126:Colostrum / Q000276:immunology; D003470:Culture Media; D004770:Enterovirus / Q000254:growth & development; D005260:Female; D005315:Fetal Diseases / Q000209:etiology / Q000276:immunology / Q000662:veterinary*; D011558:Herpesvirus 1, Suid / Q000254:growth & development; D007668:Kidney; D008431:Maternal-Fetal Exchange; D010321:Parvoviridae / Q000254:growth & development / Q000276:immunology; D011247:Pregnancy; D013552:Swine; D013553:Swine Diseases / Q000209:etiology* / Q000276:immunology; D014721:Vesicular stomatitis Indiana virus / Q000254:growth & development; D014762:Viral Interference; D014777:Virus Diseases / Q000209:etiology / Q000276:immunology / Q000662:veterinary*; D014779:Virus Replication
chemical_list: D003470:Culture Media