Distribution of estrogen receptors in subfractions of hen oviduct chromatin.
Hen oviduct chromatin was digested with DNase II and separated into two fractions. The MgCl2 insoluble chromatin fraction (43% of the total DNA) was enriched in nucleosome-like particles, which sedimented at 11 S and contained 185 base pairs of DNA. The MgCl2 soluble chromatin fraction (5% of the total DNA) was characterized by 5 S and 14 S peaks in sucrose gradients; Estrogen receptors in the chromatin fractions were labelled with [3H] estradiol using the steroid exchange assay. The concentration of receptors in the  MgCl2 soluble chromatin was 4;5 times higher than that in the MgCl2 insoluble chromatinmin sucrose gradient analysis the 11 S particles displayed a negligible specific radioactivity suggesting that estrogen receptors mainly bind to extranucleosomal chromatin.
D000818:Animals; D002467:Cell Nucleus; D002645:Chickens; D002712:Chlorides; D002843:Chromatin; D004247:DNA / Q000032:analysis; D003851:Deoxyribonucleases; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D008274:Magnesium; D010057:Oviducts / Q000648:ultrastructure; D011956:Receptors, Cell Surface
D002712:Chlorides; D002843:Chromatin; D011956:Receptors, Cell Surface; D004958:Estradiol; D004247:DNA; D003851:Deoxyribonucleases; D008274:Magnesium