Membranes of mammary gland. XI. Marker enzyme distribution profiles for membranous components from bovine mammary gland.
Enzyme distribution profiles of clarified bovine mammary homogenates separated by equilibrium centrifugation on linear sucrose gradients suggested that several of the commonly utilized marker enzymes for rat liver are also valid markers for mammary cellular components. These marker enzymes include: Succinate dehydrogenase (mitochondria), nicotinamide adenine dinucleotide phosphate cytochrome c reductase and, to a lesser extent, retenone insensitive nicotinamide adenine dinucleotide cytochrome c reductase (endoplasmic reticulum), galactosyl transferase (Golgi apparatus), 5'-nucleotidase (plasma membranes), uric acid oxidase (microbodies), and acid phosphatase (lysosomes). Rotenone sensitive nicotinamide adenine dinucleotide cytochrome c reductase and sodium, potassium, magnesium-stimulated adenosine triphosphatase were widely distributed among subcellular fractions and are not valid marker enzymes. The boyant densities determined for the above fractions should aid in design of methods to obtain enriched sources of these components for analysis.
D000818:Animals; D002417:Cattle / Q000378:metabolism*; D002458:Cell Fractionation / Q000379:methods; D002462:Cell Membrane / Q000201:enzymology; D004721:Endoplasmic Reticulum / Q000201:enzymology; D005260:Female; D006056:Golgi Apparatus / Q000201:enzymology; D007774:Lactation; D007788:Lactose Synthase / Q000032:analysis; D008247:Lysosomes / Q000201:enzymology; D008321:Mammary Glands, Animal / Q000201:enzymology*; D008566:Membranes / Q000201:enzymology; D008830:Microbodies / Q000201:enzymology; D008928:Mitochondria / Q000201:enzymology; D010088:Oxidoreductases / Q000032:analysis; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis; D011247:Pregnancy
D010088:Oxidoreductases; D007788:Lactose Synthase; D010744:Phosphoric Monoester Hydrolases