Persistent reovirus infection of CHO cells resulting in virus resistance.
We obtained a persistently infected line of Chinese hamster ovary cells by selection for resistance to reovirus infection. The cells were persistently infected by a population of viruses that were (i) cytopathic for parental chinese hamster ovary cells and (ii) similar to wild-type reovirus in molecular characteristics. The growth rate, plating efficiency, and morphology of the cells were altered. A large majority of the cells in the population were infected. There was no detectable interferon present in the medium. The cells were relatively resistant to a wide range of viruses.
D000818:Animals; D006224:Cricetinae; D003588:Cytopathogenic Effect, Viral; D004686:Encephalomyocarditis virus / Q000254:growth & development; D005260:Female; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D009522:Newcastle disease virus / Q000254:growth & development; D012087:Reoviridae / Q000254:growth & development* / Q000648:ultrastructure; D014721:Vesicular stomatitis Indiana virus / Q000254:growth & development; D014762:Viral Interference