Thymosin-induced differentiation of murine thymocytes in allogeneic mixed lymphocyte cultures.
Calf thymosin is shown to enhance the one-way MLR of CBA thymocytes cultured with allogeneic mitomycin-C- treated C57BL/J6 spleen cells. Thymosin does not enhance the one-way MLR of CBA thymocytes cultured with syngeneic mitomucin-C-treated spleen cells. Based on this finding we present a relatively simple, rapid and quantitative in vitro microculture hioassay for inducers of T-cell differentiation and propose that thymosin treatment, when accompanied by antigen presentation, induces the two-step maturational sequence of pre-T yields T1 yields T2.
D000818:Animals; D001853:Bone Marrow / Q000276:immunology; D001854:Bone Marrow Cells; D003994:Bucladesine; D002454:Cell Differentiation; D004921:Erythropoietin; D004926:Escherichia coli / Q000276:immunology; D006801:Humans; D008070:Lipopolysaccharides; D008198:Lymph Nodes / Q000166:cytology; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008937:Mitomycins; D011135:Polysaccharides, Bacterial; D012710:Serum Albumin, Bovine; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology*; D013947:Thymosin / Q000494:pharmacology*; D013949:Thymus Extracts / Q000494:pharmacology*; D013950:Thymus Gland / Q000166:cytology
D008070:Lipopolysaccharides; D008937:Mitomycins; D011135:Polysaccharides, Bacterial; D013949:Thymus Extracts; D004921:Erythropoietin; D012710:Serum Albumin, Bovine; D013947:Thymosin; D003994:Bucladesine