title: The nucleotide sequence in the promoter region of the gene N in bacteriophage lambda.
abstract: The sequence of 18 nucleotides in the region preceding the initiation of transcription of the gene N of bacteriophage lambda has been determined to be as follows (see article). The basic approach used for the sequence determination involved Escherichia coli DNA polymerase I-catalyzed elongation of the octadecanucleotide primer, dT-C-A-G-T-G-C-G-T-C-C-T-G-C-T-G-A-rU, possessing the appropriate polarity and nucleotide sequence corresponding to the 5' end of the gene N transcript. Following hybridization of the primer to the r-stand of bacteriophage lambda CI85657, sequences of the newly grown ollgonucleotide chains were determined by a) partial exonuclease digestion followed by two-dimensional fingerprinting; b) determination of pyrimidine tracts; and c) nearest neighbor analyses. Primer elongation was carried out in a controlled manner, the size of the newly grown chains being kept short by the following techniques: a) insertion of a ribonucleotide unit as the 3' terminus of the primer; b) use of a limited number of deoxynucleoside 5'-triphosphates in the elongation reaction; and c) enlongation of the primer using all the four nucleoside triphosphates with one of the triphosphates being supplied in a limiting concentration.
mesh_terms: D001483:Base Sequence; D002848:Chromatography, DEAE-Cellulose; D002855:Chromatography, Thin Layer; D003090:Coliphages; D004254:DNA Nucleotidyltransferases / Q000378:metabolism; D004279:DNA, Viral / Q000096:biosynthesis*; D004588:Electrophoresis, Cellulose Acetate; D004926:Escherichia coli / Q000201:enzymology; D005092:Exonucleases / Q000378:metabolism; D005796:Genes; D009711:Nucleotides / Q000378:metabolism; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D010761:Phosphorus Radioisotopes; D011119:Polynucleotides / Q000096:biosynthesis; D012910:Snake Venoms; D013698:Templates, Genetic; D014158:Transcription, Genetic
chemical_list: D004279:DNA, Viral; D009711:Nucleotides; D010761:Phosphorus Radioisotopes; D011119:Polynucleotides; D012910:Snake Venoms; D004254:DNA Nucleotidyltransferases; D005092:Exonucleases; D010727:Phosphoric Diester Hydrolases