Indomethacin-induced alterations in corticosteroid and prostaglandin release by isolated adrenocortical cells of the cat.
1 The effects of purported prostaglandin synthesis inhibitors on steroid and prostaglandin (E and F) release from trypsin-dispersed cat adrenocortical cells were investigated. 2 Low indomethacin concentrations potentiated adrenocorticotrophin (ACTH)-evoked prostaglandin and steroid release, whereas higher concentrations depressed both responses to ACTH. The steroidogenic response to exogenous prostaglandin E2 was not markedly altered over a wide range of indomethacin concentrations. 3 Indomethacin enhanced basal steroid release but did not enhance basal prostaglandin E or F release. 4 5,8,11,14-Eicosatetraynoic acid (ETA) elicited a concentration-dependent inhibition of ACTH-induced steroid release, but had little effect on prostaglandin E2-induced steroid release. A high concentration of ETA inhibited prostaglandin E and F release. 5 These data are discussed in relation to the concept that prostaglandins provide a critical link in ACTH-induced corticosteroidogenesis.
D015117:5,8,11,14-Eicosatetraynoic Acid / Q000494:pharmacology; D000302:Adrenal Cortex / Q000187:drug effects / Q000378:metabolism*; D000305:Adrenal Cortex Hormones / Q000378:metabolism*; D000311:Adrenal Glands / Q000378:metabolism*; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000818:Animals; D002415:Cats; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology*; D011453:Prostaglandins / Q000378:metabolism*; D013256:Steroids / Q000378:metabolism
D000305:Adrenal Cortex Hormones; D011453:Prostaglandins; D013256:Steroids; D015117:5,8,11,14-Eicosatetraynoic Acid; D000324:Adrenocorticotropic Hormone; D007213:Indomethacin