title: Comparison of JC and BK human papovaviruses with simian virus 40: DNA homology studies.
abstract: Studies were performed to ascertain the relationship of human papovavirus JC to BK virus and to simian virus 40 (SV40) by further restriction endonuclease analysis and by DNA-DNA competition hybridization on membrane filters. Form I DNA extracted from two new isolates from cases of progressive multifocal leukoencephalopathy of human papovaviruses that were JC-like in their antigenic properties were found to yield restriction endonuclease fragmentation patterns similar to those of prototypic JC virus DNA and different from those of BK or SV40. Form I DNA preparations of JC and BK viruses were found to be related to each other and to SV40 DNA to a similar extent, with JC and BK virus DNAs containing sequences homologous to both early and late regions of the SV40 genome. The relatedness in each comparison was less than 50%, and heterologous hybrids between either JC or BK and SV40 DNAs were found to be less stable than homologous SV40-SV40 hybrids in high concentrations of formamide, suggesting substantial mismatch within homologous regions, to the extent of 15 to 30%. The new JC-like isolates were also studied in competition hybridization reactions with SV40 DNA and yielded results similar to those obtained with JC virus.
mesh_terms: D001739:BK Virus / Q000032:analysis*; D001483:Base Sequence; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004279:DNA, Viral / Q000032:analysis*; D005559:Formamides / Q000494:pharmacology; D006801:Humans; D007968:Leukoencephalopathy, Progressive Multifocal / Q000382:microbiology; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000032:analysis*; D027382:Polyomaviridae; D011120:Polyomavirus / Q000032:analysis*; D013539:Simian virus 40 / Q000032:analysis*
chemical_list: D004279:DNA, Viral; D005559:Formamides; D004262:DNA Restriction Enzymes