Association of vesicular stomatitis virus glycoprotein with virion membrane: characterization of the lipophilic tail fragment.
The proteolytic enzyme, thermolysin, degraded the external segment of the membrane glycoprotein of intact vesicular stomatitis (VS) virions but left behind a small nonglycosylated fragment, presumably embedded in the virion membrane. Other proteases generated membrane-associated glycoprotein fragments differing somewhat in molecular weight. The thermolysin-resistant, virion-associated fragment, which can be selectively solubilized by either Triton X-100 or chloroform/methanol, has a molecular weight of 5,200. Amino acid analysis of the glycoprotein fragment reveals a preponderance of hydrophobic amino acids (64% of the residues); the amino-terminal amino acid is alanine as determined by dansylation. Cyanogen bromide digestion of the tail fragment generated two peptides, confirming the presence of one methionine residue per thermolysin-resistant glycoprotein fragment. The secondary structure of this glycoprotein tail peptide is maintained by at least one disulfide bridge. Thermolysin treatment is isolated VS viral glycoprotein in the presence of Triton X-100 also generated a hydrophobic peptide fragment which is very similar to the virion-associated glycoprotein fragment. The amino acid terminus of intact glycoprotein was also found to be alanine as was its dansylated Triton-micellar fragment that resisted thermolytic degradation; this finding suggests that the amino-terminal end of the VS viral glycoprotein is embedded in the virion membrane. These results suggest that the VS viral glycoprotein is an amphipathic molecule, the hydrophilic portion of which contains all the carbohydrate and a lipophilic tail segment which forms lipid or detergent micelles, thus rendering it resistant to proteolysis.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001483:Base Sequence; D001963:Bromelains / Q000378:metabolism; D002460:Cell Line; D002918:Chymotrypsin / Q000378:metabolism; D006224:Cricetinae; D003488:Cyanogen Bromide; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000032:analysis*; D007668:Kidney; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis; D011092:Polyethylene Glycols; D011402:Pronase / Q000378:metabolism; D012995:Solubility; D013381:Subtilisins / Q000378:metabolism; D013820:Thermolysin / Q000378:metabolism; D014721:Vesicular stomatitis Indiana virus / Q000032:analysis* / Q000648:ultrastructure; D014764:Viral Proteins / Q000032:analysis*
D000596:Amino Acids; D006023:Glycoproteins; D010455:Peptides; D014764:Viral Proteins; D011092:Polyethylene Glycols; D001963:Bromelains; D013381:Subtilisins; D002918:Chymotrypsin; D011402:Pronase; D013820:Thermolysin; D003488:Cyanogen Bromide