Phospholipase C assay using p-nitrophenylphosphoryl-choline together with sorbitol and its application to studying the metal and detergent requirement of the enzyme.
D002413:Cations, Divalent; D003016:Clostridium perfringens / Q000201:enzymology; D003902:Detergents / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D004789:Enzyme Activation; D005990:Glycerol / Q000494:pharmacology; D007700:Kinetics; D008345:Manganese / Q000494:pharmacology; D009596:Nitrophenols / Q000494:pharmacology; D010740:Phospholipases / Q000378:metabolism*; D010743:Phospholipids / Q000494:pharmacology; D013012:Sorbitol / Q000494:pharmacology; D015032:Zinc / Q000494:pharmacology
D002413:Cations, Divalent; D003902:Detergents; D009596:Nitrophenols; D010743:Phospholipids; D008345:Manganese; D013012:Sorbitol; D004492:Edetic Acid; D010740:Phospholipases; D015032:Zinc; D005990:Glycerol