Microtiter assay for interferon: microspectrophotometric quantitation of cytopathic effect.
This report describes an accurate, reproducible, and efficient microassay for human interferon, using a dye-uptake method to quantitate cytopathogenicity. The antiviral activity was measured by using a Gilford 300-N microsepctrophotometer, with the automatic programmer, sampler, and data lister. Two hundred interferon samples, each in a final volume of 1.0 ml, may be analyzed and recorded in 45 min. The reproducibility of a laboratory reference interferon and the human international reference B 69/19 on two different cell lines, using the model Q Oxford sampler, was found to be excellent, with the standard error of the log10 geometric mean of both references +/- 0.04 to 0.06.
D001681:Biological Assay / Q000379:methods*; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D005069:Evaluation Studies as Topic; D005347:Fibroblasts; D006801:Humans; D007372:Interferons / Q000032:analysis*; D009499:Neutral Red; D013053:Spectrophotometry; D014721:Vesicular stomatitis Indiana virus / Q000254:growth & development
D009499:Neutral Red; D007372:Interferons