Phenotypic mixing test to detect and assay avian leukosis viruses.
A phenotypic mixing (PM) test for detecting and assaying avian leukosis viruses (ALV) of the A, B, C, and D subgroups is described. An ALV and Rous sarcoma virus RSV-0) are phenotypically mixed by co-cultivating on C/O (cells susceptible to all subgroups of ALV) cells for a certain period. Then the RSV with the new virus property is assayed on C/E cells (cells resistant to infection with subgroup E leukosis/sarcoma viruses). The test is relatively simple and rapid, and its results are unequivocal. It is as sensitive as the more lengthy complement-fixation test (COFAL). The system is suitable for detecting avian leukosis viruses in samples such as heparinized blood, plasma, and embryo extracts.
D000818:Animals; D001353:Avian Leukosis / Q000382:microbiology; D001354:Avian Leukosis Virus / Q000254:growth & development* / Q000276:immunology / Q000302:isolation & purification; D001769:Blood / Q000382:microbiology; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens; D003168:Complement Fixation Tests; D003470:Culture Media; D005347:Fibroblasts; D010641:Phenotype; D013696:Temperature; D013997:Time Factors
D003470:Culture Media