Intracellular formation of analogs of cyclic AMP. Studies with brain slices labeled with radioactive derivatives of adenine and adenosine.
A variety of radioactive analogs of adenine and adenosine were incubated with guinea pig cerebral cortical slices. Neither 1,N6-etheno[14C] adenosine nor 1,N6-etheno[14C] adenine were significantly incorporated into intracellular nucleotides. 2-chloro[8-3H] adenine was incorporated, but at a very low rate and conclusive evidence for the formation of intracellular radioactive 2-chloro-cyclic AMP was not obtained. N6-Benzyl[14C] adenosine was converted only to intracellular monophosphates and significant formation of radioactive N6-benzylcyclic AMP was not detected during a subsequent incubation. 2'-Deoxy-[8-14C] adenosine was converted to both intracellular radioactive 2'-deoxy-adenine nucleotides and radioactive adenine nucleotides. Stimulation of these labeled slices with a variety of agents resulted in formation of both radioactive 2'-deoxycyclic AMP and cyclic AMP. Investigation of the effect of various other compounds on uptake of adenine or adenosine suggested that certain other adenosine analogs might serve as precursors of abnormal cyclic nucleotides in intact cells.
D000225:Adenine / Q000031:analogs & derivatives* / Q000378:metabolism; D000227:Adenine Nucleotides / Q000096:biosynthesis; D000241:Adenosine / Q000031:analogs & derivatives* / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000096:biosynthesis; D000818:Animals; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism*; D000242:Cyclic AMP / Q000031:analogs & derivatives* / Q000096:biosynthesis; D003839:Deoxyadenosines / Q000378:metabolism; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008297:Male; D014701:Veratridine / Q000494:pharmacology
D000227:Adenine Nucleotides; D003839:Deoxyadenosines; D000249:Adenosine Monophosphate; D014701:Veratridine; D000242:Cyclic AMP; D000225:Adenine; D000241:Adenosine