T lymphocyte response to H-2 mutants: cytotoxic effectors are Ly-1+2+.
The lymphocyte differentiation (Ly) antigen phenotype of cytotoxic effector T cells specific for H-2 mutant alloantigens was determined. Cytotoxic effectors generated in primary mixed lymphocyte culture and specific for H-2Kba and H-2Dda alloantigens are sensitive to both anti-Ly-1 and anti-Ly-2 serum plus complement. Reconstitution analysis demonstrated that the mutant-specific T cells were Ly-1+2+. These observations and those previously reported, which indicated that H-2K/D mutant-specific T cells proliferating in mixed lymphocyte culture were Ly-1+2+, demonstrated that Ly-1+2+ T cells are immunocompetent. Furthermore, the nature of the stimulating H-2 complex alloantigen determines the Ly phenotype of responsive T cells.
D000818:Animals; D003602:Cytotoxicity, Immunologic; D005802:Genes, MHC Class II; D006183:H-2 Antigens / Q000235:genetics*; D007519:Isoantigens / Q000235:genetics*; D008213:Lymphocyte Activation; D007959:Lymphocyte Culture Test, Mixed; D051379:Mice; D009154:Mutation; D010641:Phenotype; D013601:T-Lymphocytes / Q000276:immunology*
D006183:H-2 Antigens; D007519:Isoantigens