The biosynthesis of the free sterols and sterol esters of Neurospora crassa.
The composition of the free and esterified sterols in Neurospora crassa was examined as a function of incubation time in starvation medium containing [2-14C]mevalonic acid. The 14C incorporation was monitored in nuclear methylated and 4,14-desmethyl sterol fractions. After 7 h incubation, sterol esterification had increased from an initial 5% in the log phase culture to 48% of the total sterol pool, with a concomitant decrease in free sterols. The relationship of the free and esterified sterol components in ergosterol biosynthesis is discussed.
D004952:Esters; D007700:Kinetics; D008798:Mevalonic Acid / Q000378:metabolism; D008954:Models, Biological; D009491:Neurospora / Q000378:metabolism*; D009492:Neurospora crassa / Q000378:metabolism*; D013261:Sterols / Q000096:biosynthesis*
D004952:Esters; D013261:Sterols; D008798:Mevalonic Acid