Immunochemical comparison of estradiol-binding molecules in perinatal rat brain cytosol and serum.
Estradiol-binding macromolecules in fetoneonatal rat brain cytosol and serum were compared by immunochemical techniques. When treated by a double diffusion procedure, both cytosol and serum formed precipitin lines with rabbit antiserum specific for perinatal rat serum proteins. These lines fused completely, indicating, within the limits of detection of this particular antiserum, the presence of identical antigenic determinants in the brain and serum. Prior removal of immunoprecipitable material from cytosol or serum, after incubation with the specific antiserum, prevented formation of such precipitin lines. The procedure similarly presented specific estradiol-binding to macromolecules. It was therefore concluded that the specifically perinatal, antigenically similar, components in rat brain cytosol and serum (possibly representing alphafetoprotein) are responsible for the estradiol-binding activity in these two tissue compartments. Measurements of heme concentrations indicated that the alphafetoprotein-like material in the cytosol does not reflect blood contamination, but rather a separate population of similar or identical molecules.
D000818:Animals; D000831:Animals, Newborn; D001665:Binding Sites; D001798:Blood Proteins / Q000378:metabolism*; D001921:Brain / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D005333:Fetus; D005779:Immunodiffusion; D008297:Male; D009419:Nerve Tissue Proteins / Q000276:immunology / Q000378:metabolism*; D011233:Precipitin Tests; D011247:Pregnancy; D011485:Protein Binding; D051381:Rats; D011956:Receptors, Cell Surface
D001798:Blood Proteins; D009419:Nerve Tissue Proteins; D011956:Receptors, Cell Surface; D004958:Estradiol