Quantitative analysis of the rescue of RNA sequences by mammalian type C viruses.
The specificity and quantitation of the rescue of RNA sequences by mammalian type C viruses has been investigated. Type C virus can package with specificity only type C viral RNA. Type C viruses do not encapsidate with comparable efficiency either type B viral or cellular globin mRNA. Conversely, a non-type C mammalian retravirus, MP-MV, cannot encapsidate type C RNA. A revertant of Kirsten sarcoma virus (Ki-SV)-transformed nonproducer cells which fails to rescue biologically active Ki-SV after superinfection with helper virus had no detectable intracellular Ki-SV-specific RNA. The results suggest specific mechanisms by which type C viral proteins can package type C viral RNA and provide an approach to classifying RNA of potentially defective endogenous retraviruses as type C in origin.
D001483:Base Sequence; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D017988:Gammaretrovirus / Q000032:analysis; D005914:Globins / Q000032:analysis; D008979:Moloney murine leukemia virus / Q000032:analysis; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis; D011925:Rauscher Virus / Q000032:analysis; D011995:Recombination, Genetic; D012190:Retroviridae / Q000032:analysis
D012367:RNA, Viral; D012313:RNA; D005914:Globins