Relationship of the first step in protein synthesis to ppGpp: formation of A(5')ppp(5')Gpp.
In the presence of purified Escherichia coli lysyl-tRNA synthetase [L-lysine:tRNALys ligase (AMP-forming) EC 6.1.1.6], L-lysine, and ATP, addition of the nucleotide ppGpp results in formation of a unique product-A(5')ppp(5') Gpp. The same compound is also formed very rapidly in a cell-free protein-synthesizing system when ppGpp is added. The possible significance of this reaction in the rapid turnover of ppGpp and as a more general mechanism by which an AMP residue is activated and introduced onto a 5'-diphosphorylated species, including the 5'-end of an RNA, is further discussed.
D000227:Adenine Nucleotides / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000378:metabolism; D000469:Alkaline Phosphatase; D009189:Avian Myeloblastosis Virus / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis*; D002848:Chromatography, DEAE-Cellulose; D002855:Chromatography, Thin Layer; D004926:Escherichia coli / Q000378:metabolism*; D006150:Guanine Nucleotides / Q000378:metabolism*; D008250:Lysine-tRNA Ligase / Q000378:metabolism; D010727:Phosphoric Diester Hydrolases; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000378:metabolism; D012910:Snake Venoms; D046249:Transfer RNA Aminoacylation
D000227:Adenine Nucleotides; D001426:Bacterial Proteins; D006150:Guanine Nucleotides; D012367:RNA, Viral; D012910:Snake Venoms; D000255:Adenosine Triphosphate; D000469:Alkaline Phosphatase; D010727:Phosphoric Diester Hydrolases; D008250:Lysine-tRNA Ligase