[Kinetic properties of an alkaline phosphatase from honeybee intestine determined by a new rigorous algebraic method].
The Hill-parameters : max. velocity (VM), affinity constant (K) and resultant reaction order (n) of an alk. phosphatase (Mol. Weight 100 to 130,000) from guts of foraging worker-bees, have been calculated according to new algebraic equations allowing a rigorous determination without any extrapolation. The parameters determined from 2 strains of the same race, at 3 years interval, do not differ, and the Hill coefficients (n) are significantly lower than 1, namely : n = 0.621 +/- 0.014 (3 detn.) and n = 0,600 +/- 0,016 (t detn.) which suggest the hypothesis of a negative cooperative mechanism.
D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D001516:Bees / Q000201:enzymology*; D007422:Intestines / Q000201:enzymology; D007700:Kinetics; D008433:Mathematics; D008722:Methods
D000469:Alkaline Phosphatase
Détermination des propriétés cinétiques d'une phosphatase alcaline de l'intestin d'abeilles au moyen d'une nouvelle méthode algébrique rigoureuse.