Hormone effects on human mammary cancer in organ cultures.
Specimens of human breast cancers and fibrosarcomas were incubated for 4 to 72 hours as organ cultures. The effect of various concentrations of testosterone, progesterone, and 17-beta-estradiol on cell survival and multiplication was measured as changes in incorporation of H3-TdR into DNA. Both types of tumor reacted to the hormones; both stimulations and inhibitions were recorded. Statistical analysis of data demonstrated possible influence of factors other than hormone treatment. The specificity of hormone sensitivity assays in vitro is questioned.
D002293:Adenocarcinoma, Scirrhous / Q000473:pathology; D000368:Aged; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D004273:DNA, Neoplasm / Q000096:biosynthesis; D003864:Depression, Chemical; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells; D004848:Epithelium / Q000473:pathology; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D005354:Fibrosarcoma / Q000378:metabolism; D006801:Humans; D008875:Middle Aged; D009924:Organ Culture Techniques; D011374:Progesterone / Q000494:pharmacology*; D013268:Stimulation, Chemical; D013739:Testosterone / Q000494:pharmacology*
D004273:DNA, Neoplasm; D013739:Testosterone; D011374:Progesterone; D004958:Estradiol