[Preparation of highly puried pepsin by aminosilichrome chromatography].
The procedure for preparing highly purfied pepsin by chromatography on silicon dioxide with attached aminogroups (aminosilochrome) has been devised. Pepsin is eluated step=by-step: 0.0025 M and 0.05 M HCl. Results of disc-electrophoresis of purified pepsin bive evidence that the resultant preparation contains no admixtures. The activity of purified pepsin is 69 units per mg.
D002846:Chromatography, Affinity / Q000379:methods; D004589:Electrophoresis, Disc; D010434:Pepsin A / Q000302:isolation & purification*; D012822:Silicon Dioxide
D012822:Silicon Dioxide; D010434:Pepsin A
Poluchenie vysokoochishchennogo pepsina khromatografieĭ na aminosilokhrome