title: Appearance in vivo of single-stranded complementary ends on parental herpesvirus DNA.
abstract: Intracellular forms of pseudorabies virus parental DNA were examined before and after the onset of viral DNA synthesis. Before initiation of synthesis, parental viral DNA acquires single-stranded ends. Circular and concatemeric molecules are also observed, indicating that the single-stranded ends are complementary. Viral DNA replication is initiated at an internal site within the DNA molecule, giving rise to characteristic replicative loops with single-stranded regions in the trans position. Such replicative loops were seen in unit-size (and smaller than unit-size) linear molecules as well as in circular and concatemeric molecules. These results show that the parental viral DNA molecules that acquire single-stranded ends, and consequently are able to form circles and concatemers, proceed to replicate.
mesh_terms: D004261:DNA Replication; D004270:DNA, Circular / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000378:metabolism*; D004279:DNA, Viral / Q000096:biosynthesis / Q000378:metabolism*; D006564:Herpesviridae / Q000378:metabolism*; D011558:Herpesvirus 1, Suid / Q000378:metabolism*; D008854:Microscopy, Electron; D008970:Molecular Weight; D009690:Nucleic Acid Conformation
chemical_list: D004270:DNA, Circular; D004277:DNA, Single-Stranded; D004279:DNA, Viral