[Determination of the penicillin acylase activity of E. coli cultures using a method of gas-liquid chromatography].
Penicillinacylase activity was determined in E. coli by the product of benzylpenicillin destruction, i.e. phenylacetic acid formed under the effect of the enzyme. The determination was performed on a chromatograph. The immobile phase consisted of 10 per cent of ethylenglycol edipate on chromosorb A, modified with 2 per cent H3PO4. Nitrogen was used as the gaseous carrier. The method is rapid and handy for mass testing of the cultures with a purpose of detecting penicillinacylase-producing strains. It provided reliable determination of penicillinacylase in the cultures producing simultaneously beta-lactamase, another penicillin-destroying enzyme.
D000581:Amidohydrolases / Q000032:analysis*; D002849:Chromatography, Gas / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods; D003470:Culture Media; D004926:Escherichia coli / Q000201:enzymology*; D010397:Penicillanic Acid / Q000032:analysis; D010399:Penicillin Amidase / Q000032:analysis*; D010648:Phenylacetates / Q000032:analysis
D003470:Culture Media; D010648:Phenylacetates; D010397:Penicillanic Acid; D000581:Amidohydrolases; D010399:Penicillin Amidase
Opredelenie penitsillinatsilaznoĭ aktivnosti kul'tur E. coli s ispol'zovaniem metods gazozhidkostnoĭ khromatografii