Synthesis of an affinity chromatography column designed for recovery of labile proteins.
D001665:Binding Sites; D002846:Chromatography, Affinity / Q000379:methods*; D002855:Chromatography, Thin Layer; D003349:Cortisone Reductase / Q000302:isolation & purification*; D004355:Drug Stability; D006908:Hydroxyprogesterones; D006913:Hydroxysteroid Dehydrogenases / Q000302:isolation & purification*; D008623:Mercaptoethanol; D009243:NAD; D011485:Protein Binding; D013055:Spectrophotometry, Infrared; D013386:Succinates; D013997:Time Factors
D006908:Hydroxyprogesterones; D013386:Succinates; D009243:NAD; D008623:Mercaptoethanol; D006913:Hydroxysteroid Dehydrogenases; D003349:Cortisone Reductase