Isotachophoresis: a new technique for determination of tissue metabolite concentrations.
An electrophoretic technique, isotachophoresis, has been adapted for qualitative and quantitative analysis of metabolites in human muscle tissue. Metabolite concentrations exceeding 0.1 nmol X kg-1 wet muslce could be determined in 5-10 mg muscle biopsy samples generally in good agreement with conventional analytical methods.
D000244:Adenosine Diphosphate / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D004586:Electrophoresis / Q000379:methods*; D005958:Glucosephosphates / Q000378:metabolism; D006358:Hot Temperature; D006801:Humans; D007773:Lactates / Q000378:metabolism; D009132:Muscles / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D010725:Phosphocreatine / Q000378:metabolism; D005082:Physical Exertion; D011773:Pyruvates / Q000378:metabolism
D005958:Glucosephosphates; D007773:Lactates; D010710:Phosphates; D011773:Pyruvates; D010725:Phosphocreatine; D009243:NAD; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate