Virus-induced diabetes mellitus: reovirus infection of pancreatic beta cells in mice.
Reovirus type 3, passaged in pancreatic beta-cell cultures, produced an insulitis when inoculated into 1- to 2-week-old mice. By means of a double-label antibody technique, in which we used fluorescein-labeled antibody to reovirus and rhodamine-labeled antibody to insulin, reovirus antigens were found in beta cells. By electron microscopy, viral particles in different stages of morphogenesis were observed in insulin-containing beta cells but not glucagon-containing alpha cells. The infection resulted in destruction of beta cells, reduction in the insulin content of the pancreas, and alteration in the host's capacity to respond normally to a glucose tolerance test.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002478:Cells, Cultured; D003921:Diabetes Mellitus, Experimental / Q000209:etiology / Q000378:metabolism / Q000382:microbiology*; D003922:Diabetes Mellitus, Type 1 / Q000382:microbiology; D007328:Insulin / Q000378:metabolism; D007515:Islets of Langerhans / Q000378:metabolism / Q000382:microbiology*; D012089:Mammalian orthoreovirus 3 / Q000276:immunology; D051379:Mice; D012088:Reoviridae Infections / Q000150:complications*; D014779:Virus Replication
D000956:Antigens, Viral; D007328:Insulin