Establishment of simian sarcoma virus, type 1 (SSV-1)-transformed non-producer marmoset cell lines.
Simian sarcoma virus, type 1 (SSV-1)-transformed non-producer cell lines were established by infection of normal marmoset fibroblast cells (HF) with limiting dilutions of SSV-1. Four focus-derived cell lines were identified as non-producers by assay of culture fluids for focus-forming activity and by the mixed culture cytopathogenicity test with XC cells. Further studies failed to detect production of type-C virus by 3H-uridine labelling, reverse transcriptase assay or electron microscopy. The non-producer cell lines, designated HF/SSV-NPI, IV, V, and VI, appeared morphologically transformed and cloned in soft agar with the same efficiency as HF/SSV-1 virus-producing transformed cells. Expression of either cytoplasmic or cell surface virus-related antigens was not detected by immunofluorescence or serum cytotoxicity tests. The presence of sarcoma genome in the transformed non-producer cell lines was demonstrated by rescue of focus-forming activity following superinfection with non-transforming helper virus or by cocultivation with helper-virus-producing cell lines. The SSV-1-transformed non-producer primate cells provide a useful tool for future studies.
D000818:Animals; D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D002143:Callitrichinae; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002875:Chromosomes; D002999:Clone Cells; D003165:Complement System Proteins; D003601:Cytotoxicity Tests, Immunologic; D004273:DNA, Neoplasm / Q000302:isolation & purification; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D005347:Fibroblasts / Q000276:immunology / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D000882:Haplorhini; D012190:Retroviridae; D012842:Sarcoma Virus, Woolly Monkey; D014529:Uridine / Q000378:metabolism
D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D004273:DNA, Neoplasm; D003165:Complement System Proteins; D004259:DNA-Directed DNA Polymerase; D014529:Uridine