title: Analysis of the effects of microwave energy on enzymatic activity of lactate dehydrogenase (LDH).
abstract: Interactions between microwave energy (3 GHz) and the enzyme Lactate Dehydrogenase (LDH) have been analyzed by monitoring the enzymatic activity during irradiation in steady-state or dynamic conditions, by irradiating the sample with variable power levels (up to 6 W into the sample) and, finally, by knowing accurately the true specific absorption rate. No permanent or temporary changes can be induced when the energy absorption does not cause a temperature variation. For higher energy values, effects are purely thermal in nature. Furthermore the thermal activation of the reaction velocity, caused by microwave irradiation, is in itself sufficient to give a good fit with the experimental time evolution of the enzymatic reaction.
mesh_terms: D006358:Hot Temperature; D007770:L-Lactate Dehydrogenase / Q000378:metabolism / Q000528:radiation effects*; D008872:Microwaves; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors
chemical_list: D009243:NAD; D007770:L-Lactate Dehydrogenase