[Peptide hydrolase activity in rabbit brain subcellular fractions under conditions of adrenalectomy and administration of hydrocortisone and ACTH].
Adrenalectomy and administration of hydrocortisone and ACTH are shown to induce no changes in the total activity of neutral peptide-hydrolase in both homogenates and brain subcellular fractions. The absence of adrenalectomy and hormone administration effect on the total peptide-hydrolase activity in homogenates is established simultaneously with its essential changes in the brain subcellular fractions. A decrease in the enzymic activity of the mitochondrial-lysosomal fraction (MLF) following adrenalectomy is observed side by side with its increase in a soluble fraction. Hydrocortisone and ACTH administration cause an increase in the acid peptide-hydrolase activity in MLF and its decrease in the brain soluble and microsomal fractions of adrenalectomized rabbits. The degree of solubilization of MLF acid and neutral peptide-hydrolase by detergent triton X-100 after adrenalectomy is increased. Hydrocortisone and ACTH administration, on the contrary, decrease a degree of solubilization of the brain MLF peptide-hydrolase in adrenalectomized animals.
D000315:Adrenalectomy; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology*; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology*; D006854:Hydrocortisone / Q000494:pharmacology*; D008247:Lysosomes / Q000201:enzymology; D008297:Male; D008861:Microsomes / Q000201:enzymology; D008928:Mitochondria / Q000201:enzymology; D010447:Peptide Hydrolases / Q000378:metabolism*; D011817:Rabbits; D012995:Solubility; D013347:Subcellular Fractions / Q000201:enzymology
D000324:Adrenocorticotropic Hormone; D010447:Peptide Hydrolases; D006854:Hydrocortisone
Peptid-gidrolazna aktivnist' u subklitinnikh fraktsiiakh mozku kroliv za umov adrenalektomii ta vvedennia gidrokortizonu ĭ AKTG.