Preparation and evaluation of a noninfectious monkey pox virus vaccine.
Monkey pox virus was mechanically disrupted by low temperature and high pressure into soluble and insoluble fractions. Soluble fractions elicited virus-neutralizing antibodies (1:20 to 1:160) in rabbits, whereas the insoluble (in saline) fractions did not (less than 1:5). No infectious virus was detected after the disruption procedure. Rhesus monkeys immunized with the soluble fraction elicited virus-neutralizing (1:1,200), complement-fixing (1:16), and hemagglutinating-inhibiting (1:80 to 1:160) antibody titers and were completely protected against monkey pox virus-induced disease. This model of monkey pox virus subunit vaccine preparation may prove to be useful in developing an efficacious noninfectious vaccinia vaccine for use in high-risk individuals.
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D003168:Complement Fixation Tests; D000882:Haplorhini; D006385:Hemagglutination Inhibition Tests; D006387:Hemagglutination, Viral; D008253:Macaca mulatta; D008992:Monkey Diseases / Q000517:prevention & control; D008993:Monkeypox virus / Q000276:immunology*; D009500:Neutralization Tests; D011212:Poxviridae / Q000276:immunology*; D011213:Poxviridae Infections / Q000517:prevention & control; D014765:Viral Vaccines
D000914:Antibodies, Viral; D014765:Viral Vaccines