Rapid identification and subtyping of herpes simplex virus by complement-dependent cytotoxicity.
A simple method for rapid identification and subtyping of herpes simplex virus from clinical specimens is presented. Inoculated cultures demonstrating cytopathic changes were treated with type-specific cytotoxic antisera against herpes simplex virus and complement. Lysis of cells was revealed by staining with trypan blue. The technique allowed identification and subtyping of herpes simplex virus within 2 h after the appearance of CPE.
D003165:Complement System Proteins; D003601:Cytotoxicity Tests, Immunologic; D006561:Herpes Simplex / Q000382:microbiology*; D006801:Humans; D012703:Serotyping / Q000379:methods*; D018139:Simplexvirus / Q000145:classification*
D003165:Complement System Proteins