title: Identification of a polypeptide encoded by the avian sarcoma virus src gene.
abstract: Two techniques were used to search for the polypeptide encoded by the avian sarcoma virus (ASV) src gene. First, antiserum from rabbits bearing ASV-induced fibrosarcomas was used to immunoprecipitate a transformation-specific antigen from ASV-transformed chick embryo fibroblasts. This antigen has an apparent molecular weight (Mr) of 60,000. Second, the 3' one-third of the ASV genome, selected by oligo(dT)-cellulose chromatography and sucrose gradient sedimentation, was translated in a mRNA-dependent reticulocyte cell-free lysate. This RNA species programmed the synthesis of a polypeptide that comigrated with the transformation-specific antigen of Mr 60,000 immunoprecipitated from transformed cells. The methionine-containing tryptic peptides from the polypeptides of Mr 60,000 obtained from translation in vitro and from immunoprecipitation were found to be identical upon two-dimensional fractionation.
mesh_terms: D001355:Alpharetrovirus / Q000235:genetics*; D002460:Cell Line; D002472:Cell Transformation, Viral; D002474:Cell-Free System; D005814:Genes, Viral; D007158:Immunologic Techniques; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics* / Q000276:immunology
chemical_list: D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins