title: Effects of prostaglandin E1 and indomethacin on ACTH, prolactin, GH and LH from rat pituitary in vitro.
abstract: The effects of prostaglandin E1 (PGE1) and indomethacin (IDM) on the release of several pituitary hormones from the rat pituitary were investigated in vitro. An addition of 2 microng/ml of PGE1 to the medium elicited the release of growth hormone (GH) and prolactin, but not of adrenocorticotropin (ACTH) and luteinizing hormone (LH). Although the addition of 1 microng/ml of IDM alone resulted in no effect on the basal release of these hormones, IDM diminished the release of ACTH induced by crude rat hypothalamic extracts (HE) or lysine-8-vasopressin (LVP), and LH induced by HE or luteinizing hormone-releasing hormone (LH-RH). These findings implicate that a part of PGE1 action might be a direct one on the pituitary gland and PGE1 might release GH and prolactin, whereas IDM might have a direct action on the pituitary gland, and that blunt the release of these pituitary hormones induced by several stimuli.
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000032:analysis; D000818:Animals; D013006:Growth Hormone / Q000032:analysis; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology*; D007986:Luteinizing Hormone / Q000032:analysis; D008297:Male; D010904:Pituitary Gland, Posterior / Q000032:analysis*; D011388:Prolactin / Q000032:analysis; D011458:Prostaglandins E / Q000494:pharmacology*; D051381:Rats
chemical_list: D011458:Prostaglandins E; D000324:Adrenocorticotropic Hormone; D011388:Prolactin; D007986:Luteinizing Hormone; D013006:Growth Hormone; D007213:Indomethacin