The reduction of Pseudomonas cytochrome c551 oxidase by chromous ions.
The reduction  of cytochrome c551 oxidase from Pseudomonas aeruginosa by Cr2+ ions was followed in the stopped-flow apparatus at a number of wavelengths. The c-haem reduction proceeded in a biphasic fashion with second-order rate constants of 2.6 X 10(5)M-1-S-1 and 4.8 X 10(4)M-1-S-1 at 25 degrees C, whereas the biphasic reduction of the d1-haem appeared to be independent of reductant concentration with rate constants of approx. 1.0S-1 and 0.25S-1 respectively. The kinetically determined difference spectra (reduced minus oxidized) for the c- and d1-haems are presented.
D002857:Chromium; D003576:Electron Transport Complex IV / Q000378:metabolism*; D007700:Kinetics; D010084:Oxidation-Reduction; D011550:Pseudomonas aeruginosa / Q000201:enzymology*
D002857:Chromium; D003576:Electron Transport Complex IV