Purification of herpesvirus nucleocapsids by fluorocarbon extraction.
Purified nucleocapsids were prepared from herpes simplex virus type-1 and herpes simplex virus type-2 infected cells, using a fluorocarbon extraction technique with Freon 113. Fifty to 85% of the total nucleocapsid population remained 'nucleated' or full in terms of the nucleic acid core making them useful as a source of DNA. CsCl banded DNA represented 30--40% of the theoretical yield of the total nucleocapsid population.
D002213:Capsid / Q000302:isolation & purification*; D002460:Cell Line; D005617:Chlorofluorocarbons, Methane; D003588:Cytopathogenic Effect, Viral; D004279:DNA, Viral / Q000302:isolation & purification*; D018139:Simplexvirus / Q000032:analysis* / Q000254:growth & development / Q000648:ultrastructure; D014764:Viral Proteins / Q000302:isolation & purification*; D014779:Virus Replication
D005617:Chlorofluorocarbons, Methane; D004279:DNA, Viral; D014764:Viral Proteins