title: Saturable and nonsaturable process of sugar uptake: effect of oncogenic transformation in transport and uptake of nutrients.
abstract: Uptake of sugars into cells by a saturable process increased enormously during and after transformation, and uptake by a nonsaturable process increased significantly but less remarkably compared to controls. The drastic change of uptake rates, observed at around 5 x 10(-3) M sugar during and after transformation, emphasizes the significant observation that transition of the sugar uptake system from a saturable to a nonsaturable process occurs near the physiological concentration of D-glucose normally seen in animal blood. At concentrations below higher than 5 x 10(-3) M, where a saturable process is barely involved, nonsaturable uptakes of D-glucose, D-mannose, D-galactose, 2-deoxy-D-glucose and 3-O-methyl-D-glucose proceed tens to hundreds fold faster than the rate of simple diffusion of L-glucose. These findings suggest that nonsaturable uptake of the sugars known to be substrates for the saturable transport carrier system may not be a physical process or simple diffusion, as observed for L-glucose uptake. Rather, the nonsaturable uptake might be part of the total physiological process which, along with the saturable process, is controlled by a membrane-coordination mechanism. A plausible mechanism is discussed in which negative cooperativity of nutrient uptake, such as that found in bacteria, is involved.
mesh_terms: D000818:Animals; D001693:Biological Transport, Active; D050260:Carbohydrate Metabolism; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured / Q000378:metabolism*; D003847:Deoxyglucose / Q000378:metabolism; D004058:Diffusion; D005347:Fibroblasts; D005690:Galactose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D008358:Mannose / Q000378:metabolism; D008757:Methylglucosides / Q000378:metabolism; D051379:Mice; D009053:Sarcoma Viruses, Murine; D013237:Stereoisomerism
chemical_list: D008757:Methylglucosides; D003847:Deoxyglucose; D005947:Glucose; D008358:Mannose; D005690:Galactose