Influence of defective virion core proteins on RNA maturation with an avian sarcoma virus.
The RNA of the avian sarcoma virus B77 temperature-sensitive mutant LA334 was investigated using electrophoretic analysis. The RNA from mutant virus grown at the nonpermissive temperature (42degrees C) showed a heterogeneous peak between 80 and 125S, and another at about 35S. The RNA of the mutant virus grown at the permissive temperature (35 degrees C) behaved like wild-type B77 virus RNA, exhibiting a major peak at 70S. The homology between the various RNA fractions and virus-specific DNA probe was determined, indicating that mutant virus grown at the nonpermissive temperature contains relatively large amounts of nonviral-specific RNA.
D001358:Avian Sarcoma Viruses / Q000254:growth & development / Q000378:metabolism*; D009154:Mutation; D009690:Nucleic Acid Conformation; D012367:RNA, Viral / Q000096:biosynthesis*; D013696:Temperature; D014764:Viral Proteins / Q000096:biosynthesis*
D012367:RNA, Viral; D014764:Viral Proteins