title: Synthesis of ribonucleotides and their participation in ribonucleic acid synthesis by Coxiella burnetii.
abstract: Synthesis of ribonucleic acid (RNA) by the deoxyribonucleic acid-dependent RNA polymerase of Coxiella burnetii required adenosine, uridine, guanosine, and cytidine 5'-triphosphates. Cell-free preparations of this obligate intracellular procaryotic parasite had competence to phosphorylate ribonucleoside mono- and diphosphates in the presence of exogenous adenosine and guanosine 5'-triphosphates to the corresponding di- and triphosphates. C. burnetii contained about 2 nmol of adenosine 5'-triphosphate per mg of protein, which could serve as a approximately P donor for in vivo synthesis of nucleoside triphosphates. The latter were then used as substrates in the synthesis of RNA in a coordinated metabolic system with C. burnetii RNA polymerase. It is suggested that during infection the rickettsiae might obtain the nucleotides necessary for RNA synthesis from the vacuoles in which C. burnetii proliferates.
mesh_terms: D000255:Adenosine Triphosphate / Q000096:biosynthesis*; D003381:Coxiella / Q000378:metabolism*; D003597:Cytosine Nucleotides / Q000096:biosynthesis*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000096:biosynthesis*; D009701:Nucleoside-Diphosphate Kinase / Q000378:metabolism; D012329:RNA, Bacterial / Q000096:biosynthesis*; D014500:Uracil Nucleotides / Q000096:biosynthesis*; D014544:Uridine Triphosphate / Q000096:biosynthesis*
chemical_list: D003597:Cytosine Nucleotides; D012329:RNA, Bacterial; D014500:Uracil Nucleotides; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D009701:Nucleoside-Diphosphate Kinase; D012321:DNA-Directed RNA Polymerases; D014544:Uridine Triphosphate