The role of metabolic energy in the lethal action of basic proteins on Candida albicans.
Comparative studies were made on the destructive effects of certain basic proteins on a strain of Candida albicans and two of its respiration-impaired mutants. Both by direct plate counts of survivors and by quantitative ultraviolet spectrophotometric analyses of released cellular constituents, the respiration-impaired mutants were less vulnerable to the destructive actions of the basic proteins than were ordinary wild-type cells. The lethal incidence and the ultraviolet absorbing cellular substances released from wild-type cells by the proteins were markedly decreased in the presence of the oxidative phosphorylation uncouplers sodium azide, 2,4-dinitrophenol, and salicylanide and approximately equal to the effects produced on an oxidative phosphorylation mutant not treated with the uncouplers. The heightened resistance of a culture through mutational or chemical impairment of its respiratory system suggests a role of metabolic energy in the destructive action of various basic proteins on yeast cells.
D001386:Azides / Q000494:pharmacology; D002176:Candida albicans / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D002462:Cell Membrane / Q000187:drug effects / Q000648:ultrastructure; D003574:Cytochrome c Group / Q000494:pharmacology; D004140:Dinitrophenols / Q000494:pharmacology; D006801:Humans; D009113:Muramidase / Q000494:pharmacology; D009154:Mutation; D010085:Oxidative Phosphorylation / Q000187:drug effects; D010101:Oxygen Consumption / Q000187:drug effects; D011479:Protamines / Q000494:pharmacology; D011506:Proteins / Q000494:pharmacology*; D012458:Salicylanilides / Q000494:pharmacology; D012709:Serum Albumin / Q000494:pharmacology; D012710:Serum Albumin, Bovine / Q000494:pharmacology
D001386:Azides; D003574:Cytochrome c Group; D004140:Dinitrophenols; D011479:Protamines; D011506:Proteins; D012458:Salicylanilides; D012709:Serum Albumin; D012710:Serum Albumin, Bovine; D009113:Muramidase