The role of the blood in metabolism of prostaglandin E1 in the cat lung.
We found that when 15-keto-PGE1 was added to cat blood, it was converted to 13, 14-dihydro-15-keto-PGE1 (dihydro-keto-PGE1) by a NADH-dependent enzyme associated with some formed element(s) in the blood. When PGE1 was injected into the pulmonary artery of blood-perfused lungs, the only metabolite detectable in the pulmonary venous blood was the dihydro-keto-PGE1. However, when the lungs were perfused with an artificial perfusate containing no blood cells, a small amount of 15-keto-PGE1 was detected in the venous effluent. Therefore it would appear that a blood-borne delta13 reductase was partially responsible for the conversion of PGE1 to dihydro-keto-PGE1 on passage through blood-perfused cat lungs.
D000818:Animals; D002415:Cats; D008168:Lung / Q000502:physiology*; D009243:NAD / Q000502:physiology; D010477:Perfusion; D011458:Prostaglandins E / Q000097:blood / Q000378:metabolism*; D011465:Prostaglandins, Synthetic / Q000097:blood / Q000378:metabolism
D011458:Prostaglandins E; D011465:Prostaglandins, Synthetic; D009243:NAD