Vesicular stomatitis virus-infected L1210 murine leukemia cells: increased immunogenicity and altered surface antigens.
Homogenates of L1210 cells infected in vitro with vesicular stomatitis virus (VSV) were immunogenic agains a tumor graft of 100 times the LD50 dose of L1210 cells" whereas those of uninfected cells were not. The immunogenicity of intact X-irradiated L1210 cells was distinguishable from that of VSV-infected cell homogenates on the basis of the susceptibility of immunogenicity to experimental procedures used in preparation of the immunogenic homogenates: Homogenization of intact X-irradiated cells or their infection with VSV prior to irradiation led to loss of immunogenicity. In addition, uninfected cell homogenates were not made immunogenic nor was the immunogenicity of VSV-infected cell homogenates eliminated by X-irradiation. At the time of tumor challenge, sera from mice that were effectively immunized with VSV-infected cell homogenate showed a high VSV-neutralizing titer but no complement-dependent cytotoxicity for L1210 cells. Quantitative absorption studies demonstrated that VSV infection led to a marked reduction in L1210 surface antigens recognized by cytotoxic alloantibody; spatial association between these antigens and VSV antigens was not demonstrable on VSV-infected cells. Antigens recognized by heterologous antiserum to L1210 cells were also reduced following VSV infection.
D000818:Animals; D000917:Antibody Formation; D000951:Antigens, Neoplasm / Q000528:radiation effects; D000956:Antigens, Viral; D002462:Cell Membrane / Q000276:immunology; D002478:Cells, Cultured; D005260:Female; D006084:Graft Rejection; D006649:Histocompatibility Antigens / Q000528:radiation effects; D007109:Immunity; D007114:Immunization; D007939:Leukemia L1210 / Q000276:immunology* / Q000382:microbiology; D051379:Mice; D008811:Mice, Inbred DBA; D014721:Vesicular stomatitis Indiana virus / Q000276:immunology*; D014779:Virus Replication
D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D006649:Histocompatibility Antigens