In vitro activation of a soluble cholesterol esterase from bovine adrenals by a cAMP-dependent protein kinase.
Properties and partial purification of the bovine adrenal cholesterol esterase from the 100000 X g supernatant fraction were investigated. Variations of the enzyme activity with time-dependent (enzymatic) and time-dependent (non enzymatic) effects have been demonstrated. Mg2 has been proved to inhibit the enzyme activity by a non-enzymatic effect in 50mM Tris/HCl buffer, pH 7.4. A time-dependent inactivation of the cholesterol esterase has been observed in the same buffer. The enzyme could be protected from this enzymatic inactivation by its substrate, cholesterol oleate. cAMP, ATP and Mg2 cuase a time-dependent stimulation of the enzyme in 50mM Tris/HCl buffer, pH 7.4. This result suggests that corticotropin activates the soluble cholesterol esterase from bovine adrenals via cAMP-dependent protein kinase. This view is strengthened by the incorporation of 32P radioactivity from [gamma-32P] ATP into the protein fraction of the 100,000 X g supernatant. The protein-bound 32P radioactivity could be co-purified with the enzyme activity during the partial purification of the soluble cholesterol esterase.
D000311:Adrenal Glands / Q000201:enzymology* / Q000648:ultrastructure; D000818:Animals; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D002417:Cattle; D000242:Cyclic AMP / Q000378:metabolism*; D004789:Enzyme Activation; D066298:In Vitro Techniques; D010743:Phospholipids / Q000302:isolation & purification; D011494:Protein Kinases / Q000378:metabolism*; D011506:Proteins / Q000302:isolation & purification; D002787:Sterol Esterase / Q000302:isolation & purification / Q000378:metabolism*; D013347:Subcellular Fractions / Q000201:enzymology; D013997:Time Factors
D010743:Phospholipids; D011506:Proteins; D000242:Cyclic AMP; D011494:Protein Kinases; D002265:Carboxylic Ester Hydrolases; D002787:Sterol Esterase