Chemiluminescence spectra of human myeloperoxidase and polymorphonuclear leukocytes.
The light emission spectra of myeloperoxidase-H2O2-Cl- and phagocytizing polymorphonuclear leukocytes were estimated by a computer simulation technique by using light transmittance data from nine band-pass filters. No shift in the chemiluminescence spectrum of either system was observed during the course of the reactions, suggesting that the light-generating mechanisms remain constant after their initiation. Transmittances were virtually identical for both myeloperoxidase-H2-O2-Cl- and polymorphonuclear leukocyte reactions, suggesting that the light-generating mechanisms are identical and leading to the estimation of nearly identical spectra. Both spectra were broad, with maximum light emission near 570 nm.
D003201:Computers; D006801:Humans; D008163:Luminescent Measurements; D009504:Neutrophils / Q000502:physiology*; D009195:Peroxidase / Q000378:metabolism*; D010544:Peroxidases / Q000378:metabolism*; D010587:Phagocytosis; D013057:Spectrum Analysis
D010544:Peroxidases; D009195:Peroxidase