[Obtaining spheroplasts from the agents of glanders and melioidosis and separation of membrane structures from them].
Spheroplasts were obtained from the causative agents of glanders and melioidosis under the effect of lysozyme and antibiotics. In the capacity of an inducing agent lysozyme was effective in high concentration only (0.4%); preliminary washing and incubation in sucrose were necessary to obtain glanders spheroplasts. Of the antibiotics studied penicillin was more useful for obtaining melioidosis spheroplasts and ampicillin--for glanders spheroplasts. Membrane preparations were derived from the spheroplasts of glanders and melioidosis causative agents.
D000667:Ampicillin / Q000494:pharmacology; D002462:Cell Membrane / Q000648:ultrastructure; D009113:Muramidase / Q000494:pharmacology; D010406:Penicillins / Q000494:pharmacology; D011549:Pseudomonas / Q000187:drug effects / Q000648:ultrastructure; D012310:Ristocetin / Q000494:pharmacology; D013104:Spheroplasts / Q000648:ultrastructure
D010406:Penicillins; D012310:Ristocetin; D000667:Ampicillin; D009113:Muramidase
Poluchenie sferoplastov iz vozbuditeleĭ sapa i melioidoza i vydelenie iz nikh membrannykh struktur.