Ertosterol biosynthesis in Saccharomyces cerevisiae: mutants deficient in the early steps of the pathway.
Thermosensitive mutants, auxotrophic for ergosterol synthesis, have been isolated, analyzed genetically and their enzymatic deficiencies investigated. These mutants were classified into seven unlinked complementation groups. These groupes lack the following enzymatic activities: squalene epoxidase (erg 1), 2,3-oxidosqualene-lanosterol cyclase (erg 7), phosphomevalonic kinase (erg 8), mevalonic kinase (erg12) and squalene synthetase (erg 9, erg 10, erg 11).
D004852:Epoxy Compounds; D004875:Ergosterol / Q000096:biosynthesis*; D013186:Farnesyl-Diphosphate Farnesyltransferase / Q000378:metabolism; D006358:Hot Temperature; D019751:Intramolecular Transferases; D007535:Isomerases / Q000378:metabolism; D007810:Lanosterol; D008798:Mevalonic Acid / Q000378:metabolism; D009154:Mutation; D010105:Oxygenases / Q000378:metabolism; D010770:Phosphotransferases / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D013185:Squalene / Q000378:metabolism
D004852:Epoxy Compounds; D007810:Lanosterol; D013185:Squalene; D010105:Oxygenases; D013186:Farnesyl-Diphosphate Farnesyltransferase; D010770:Phosphotransferases; D007535:Isomerases; D019751:Intramolecular Transferases; C021924:lanosterol synthase; D008798:Mevalonic Acid; D004875:Ergosterol