[Induction of endogenous murine C-type viruses. Attempt at discrimination by the pI of polypeptide p30 and the aspect of cellular transformation].
Endogenous ecotropic and xenotropic murine C-type viruses induced in K-Balb-3T3 cells treated with iododeoxyuridine (IdU) were selected by infection of appropriate indicator cells. The isoelectric point (p1) of the major viral polypeptide (p30) was found to be 6.1 for the ecotropic virus (class I), and 5.7 for the xenotropic virus (class II). An isoelectric form (iso p30) of pl 6.5 was observed in the initial induction peak. In addition, the pattern of cellular alteration in NRK cells at its onset varied according to the pseudotype, the class I pseudotype inducing round cell foci while the foci associated with the class II pseudotype consisted of fusiform cells.
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D007065:Idoxuridine / Q000494:pharmacology; D007526:Isoelectric Point; D051379:Mice; D008807:Mice, Inbred BALB C / Q000382:microbiology; D012190:Retroviridae / Q000032:analysis / Q000254:growth & development; D009053:Sarcoma Viruses, Murine / Q000254:growth & development; D014764:Viral Proteins; D014779:Virus Replication / Q000187:drug effects
D014764:Viral Proteins; D007065:Idoxuridine
Induction de virus endogènes murins de type C. Essai de discrimination par le pI du polypeptide p30 et l'aspect de la transformation cellulaire.