Analysis of murine C-type virus structural proteins by rocket and crossed immunoelectrophoresis.
We have examined the structural proteins of Rauscher murine leukemia virus (R-MuLV) by means of rocket immunoelectrophoresis and crossed immunoelectrophoresis, using polyspecific antisera to Tween/ether-disrupted purified R-MuLV. Fifteen different precipitation lines were recognized in virus lysates. Using five reference antisera prepared to purified R-MuLV-structural proteins, the precipitation lines of p 10, p 15, p 30 and gp 69/70 were identified. These techniques, although less sensitive than radioimmunoassay, have several advantages, such as simplicity, direct control of precipitation reactions and possibility of using crude antigen preparations.
D000818:Animals; D000918:Antibody Specificity; D000951:Antigens, Neoplasm; D007106:Immune Sera; D007122:Immunoelectrophoresis / Q000379:methods; D007123:Immunoelectrophoresis, Two-Dimensional; D009052:Leukemia Virus, Murine / Q000276:immunology; D051379:Mice; D009174:Mycoplasma / Q000276:immunology; D011234:Precipitins; D012190:Retroviridae / Q000276:immunology*; D012756:Sheep
D000951:Antigens, Neoplasm; D007106:Immune Sera; D011234:Precipitins