[The preparation of three dehydrogenases and two kinases from pig heart by a single procedure (author's transl)].
A single procedure for the preparation of lactate dehydrogenase (EC 1.1.1.27), the mitochondrial and cytoplasmic forms of malate dehydrogenase (EC 1.1.1.37), adenylate kinase (EC 2.7.4.3) and pyruvate kinase (EC 2.7.1.40) from pig heart is described. The five enzymes are obtained in preparative amounts in homogenous form with specific activities equal to or higher than those previously reported. Some molecular properties of pig heart pyruvate kinase are determined.
D000263:Adenylate Kinase / Q000302:isolation & purification; D000818:Animals; D007770:L-Lactate Dehydrogenase / Q000302:isolation & purification; D008291:Malate Dehydrogenase / Q000302:isolation & purification; D008722:Methods; D008928:Mitochondria / Q000201:enzymology; D009206:Myocardium / Q000201:enzymology*; D010088:Oxidoreductases / Q000302:isolation & purification*; D010770:Phosphotransferases / Q000302:isolation & purification*; D011770:Pyruvate Kinase / Q000302:isolation & purification; D013552:Swine
D010088:Oxidoreductases; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D010770:Phosphotransferases; D011770:Pyruvate Kinase; D000263:Adenylate Kinase
Gemeinsame Präparation von drei Dehydrogenasen und zwei Kinasen in einem Arbeitsgang aus Schweineherz