title: Hemagglutination and hemagglutination-inhibition studies with a strain of Nebraska calf diarrhea virus (bovine rotavirus).
abstract: A hemagglutinin has been prepared from Nebraska calf diarrhea virus (NCDV) propagated in BS-C-1 cell line. After cesium chloride centrifugation, the hemagglutinin of the bovine rotavirus was found to be associated with intact virions (density 1.355 g/ml) but not with virions lacking an outer capsid layer (density 1.375 g/ml). In hemagglutination-inhibition (HAI) tests, the hemagglutinin reacted specifically with NCDV serum, and HAI seroconversions were detected in some sera tested. Cross-reactions were observed in complement fixation tests between the human and bovine rotaviruses but were not demonstrated by HAI, suggesting that the hemagglutinin detects a specific rather than a group antibody response.
mesh_terms: D000818:Animals; D002417:Cattle; D002460:Cell Line; D002648:Child; D003429:Cross Reactions; D005759:Gastroenteritis / Q000276:immunology / Q000382:microbiology; D006385:Hemagglutination Inhibition Tests; D006386:Hemagglutination Tests; D006389:Hemagglutinins, Viral / Q000032:analysis; D006801:Humans; D012328:RNA Viruses / Q000276:immunology*; D012401:Rotavirus / Q000276:immunology* / Q000648:ultrastructure
chemical_list: D006389:Hemagglutinins, Viral