Interaction between cyclic nucleotides and herpes simplex viruses: productive infection.
Infection of human fibroblasts and HEp-2 cells with herpes simplex virus type 1 (HSV-1) produced a decrease in the intracellular levels of cyclic adenosine 5'- monophosphate (cAMP) and a concomitant increase in the cyclic guanosine 5'- monophosphate (cGMP) levels. In both cell cultures, changes in cyclic nucleotide levels were first observed at 6 h after viral inoculation and were maximal at 12 h. In human fibroblasts, the addition of theophylline, dibutyryl cAMP, or papaverine (cAMP-enhancing compounds) decreased significantly the yield of HSV-1, whereas the addition of insulin or dibutyryl cGMP (cGMP-enhancing compounds) increased the viral yield. In HEp-2 cells, only theophylline decreased the yield of HSV-1, and the cGMP-enhancing compounds had no apparent effect. Cyclic nucleotide enhancing compounds exhibited their effect only if added to either cell culture within the first 3 h after inoculation with HSV-1.
D003994:Bucladesine / Q000494:pharmacology; D002460:Cell Line; D000242:Cyclic AMP / Q000378:metabolism*; D006152:Cyclic GMP / Q000378:metabolism*; D003995:Dibutyryl Cyclic GMP / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D010208:Papaverine / Q000494:pharmacology; D018139:Simplexvirus / Q000187:drug effects / Q000254:growth & development*; D013806:Theophylline / Q000494:pharmacology
D007328:Insulin; D003995:Dibutyryl Cyclic GMP; D003994:Bucladesine; D013806:Theophylline; D010208:Papaverine; D000242:Cyclic AMP; D006152:Cyclic GMP