Feto-maternal amino acid patterns and cyclic AMP in the human placenta with abnormal pregnancies, particularly with SFD.
To clarify the causes of SFD from the view-point of placental function, investigations were made on feto-maternal amino acid circumstances and on how cyclic AMP behaves in the placenta, particularly in relation to the transport of amino acid across the placenta. Amino acids were measured by gas liquid chromatography (hydrogen-flame ion detector), c-AMP by the binding protein method, adenyl cyclase activity by Krishna's method, and proteins by Lowry's method. Results show: (1) The levels of maternal amino acids were indistinctive between the SFD group and the normal pregnancy group. (2) The feto-maternal ratio of amino acid concentrations (f/m ratio) was lower in the SFD group than in the normal control. (3) The level of c-AMP in the placenta was lower in the SFD group than in the control. (4) The lower level of placenta c-AMP in the SFD placenta seems partly attributable to the decreased adenyl cyclase activity. (5) There was a positive correlation (gamma=0.61) between the content of c-AMP in the placenta and the f/m ratio of amino acid concentrations. These findings suggest a decline in placental active transport of acids in SFD; adenyl cyclase and c-AMP in the placenta presumably play a key role in such course.
D000262:Adenylyl Cyclases / Q000032:analysis; D000596:Amino Acids / Q000097:blood*; D000242:Cyclic AMP / Q000032:analysis*; D005260:Female; D005312:Fetal Blood / Q000032:analysis; D006801:Humans; D007230:Infant, Low Birth Weight; D007231:Infant, Newborn; D007236:Infant, Small for Gestational Age; D008431:Maternal-Fetal Exchange; D010920:Placenta / Q000201:enzymology; D011225:Pre-Eclampsia / Q000378:metabolism; D011247:Pregnancy; D011273:Pregnancy, Prolonged; D011458:Prostaglandins E / Q000494:pharmacology
D000596:Amino Acids; D011458:Prostaglandins E; D000242:Cyclic AMP; D000262:Adenylyl Cyclases