title: Characterization of the allergenic components of the house dust mite, Dermatophagoides farinae.
abstract: Characterization and purification of the allergenic components of the house dust mite, Dermatophagoides farinae, were investigated. It was found that the mite antigen was heat-stable, pronase-sensitive, and periodate-resistant, which suggested that the antigenic determinants of the mite antigen resided mainly in the protein component. Purification of the mite antigen was tried using Sephadex G-150, DEAE cellulose, and isoelectrofocusing columns. The most potent allergenic fraction was around 20,000-30,000 in molecular weight, although the allergenic activity spread in a wide molecular weight range. Prausnitz-Küstner type skin reactions were performed on rats using mouse homocytotropic antibodies for allergenic evaluation, and the clinical usefulness of this method is discussed.
mesh_terms: D000485:Allergens; D000596:Amino Acids; D000645:Ammonium Sulfate / Q000494:pharmacology; D000818:Animals; D000941:Antigens / Q000302:isolation & purification; D002241:Carbohydrates; D005591:Chemical Fractionation; D011232:Chemical Precipitation; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004391:Dust; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008925:Mites / Q000276:immunology*; D010504:Periodic Acid / Q000494:pharmacology; D011402:Pronase / Q000494:pharmacology; D051381:Rats; D014238:Trichloroacetic Acid / Q000494:pharmacology
chemical_list: D000485:Allergens; D000596:Amino Acids; D000941:Antigens; D002241:Carbohydrates; D004391:Dust; D010504:Periodic Acid; D014238:Trichloroacetic Acid; D011402:Pronase; D000645:Ammonium Sulfate