Physical and metabolic requirements for early interaction of poliovirus and human rhinovirus with HeLa cells.
Attachment, ""tight binding'' and eclipse of radioactive poliovirus 2 (P2) and human rhinovirus 2 (HRV 2) were investigated. The activation energy for attachment of both HRV2 and P2 was about 13 kcal/mol. HRV2 differed from P2 in two respects: the Arrhenius plot for attachment of HRV2 showed a break at 15 to 19 degrees C when the cells were first treated several hours at 0 degrees C, and attachment of HRV2 was inhibited by treatment of cells with metabolic poisons able to reduce cellular ATP by more than 90%. Tight binding was determined by isolation of a specific P2-membrane complex or by loss of EDTA dissociability of HRV2. Tight binding of both viruses was slowed by 0.01 M iodoacetamide but not by 0.02 M F-; F- plus 0.002 M CN- slowed tight binding of HRV2 but not of P2. Eclipse, the irreversible alteration of parental virions, was detected by isolation of cell-associated subviral particles or by loss of cell-associated infectious virus. Eclipse of both viruses is slowed by iodoacetamide or F-. It seems likely that the early steps of infection with picornaviruses may be sensitive to alterations in the cell membrane produced by metabolic inhibitors or by treatment at low temperature.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000327:Adsorption; D002462:Cell Membrane / Q000382:microbiology; D003486:Cyanides / Q000494:pharmacology; D005459:Fluorides / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D007460:Iodoacetamide / Q000494:pharmacology; D008954:Models, Biological; D017955:Poliovirus / Q000254:growth & development*; D012229:Rhinovirus / Q000254:growth & development*; D013696:Temperature; D014779:Virus Replication / Q000187:drug effects
D003486:Cyanides; D000255:Adenosine Triphosphate; D005459:Fluorides; D007460:Iodoacetamide