[Demonstration of the agent of bovine viral diarrhea-mucosal disease (BVD/MD) in comparison between organ fluoresence freezing method and the direct cultivation in tissue culture].
Organs of 100 calves whose clinical symptoms indicated a BVD/MD viral infection have been examined in the laboratory by different methods on BVD/MD virus in order to compare the effectivity of the single test-systems. By inoculation of organ material into tissue culture from foetal calf kidneys and additional staining with swine-fever conjugate in the CCSC-system 59 calves were detected as BVD/MD virus carriers. Taking this result for comparative purposes equal to 100% there could be stated an effectivity of 73% by inoculation of tissue cultures and judging cpe instead of staining with fluorescent antibody as above. Only 34% reactions could be demonstrated by means of heterotypic immunofluorescence in organ tissues. For diagnostic purposes a combination of the easy and quick method of heterotypic immunofluorescence in organ tissues and a cultural virus isolation from organ material with additional immunofluorescence is recommended.
D000818:Animals; D002417:Cattle; D046508:Culture Techniques; D001908:Diarrhea Viruses, Bovine Viral / Q000302:isolation & purification*; D005455:Fluorescent Antibody Technique; D008722:Methods; D012328:RNA Viruses / Q000302:isolation & purification*; D014776:Virus Cultivation
Der Erregernachweis der Bovinen Virusdiarrhoe-Mucosal Disease (BVD/MD) im Vergleich zwischen der Organ-Fluoreszenz-Gefrierschnittmethode und der Direktanzüchtung in der Gewebekultur.