Chromosome banding, isoenzyme studies and determination of Epstein-Barr virus DNA content on human Burkitt lymphoma/mouse hybrids.
Four independently fused hybrid clones derived from a cross between the mouse mammary carcinoma TA3Ha and the human Burkitt lymphoma line Daudi were tested for the EBV-determined nuclear antigen (EBNA), EBV-DNA and the presence of human chromosomes, in the course of serial propagation in vitro. EBNA and EBV-DNA were lost in parallel with the loss of human chromosomes. It seems that the persistence of EB-viral genetic information does not require the presence of a specific human chromosome(s) in this particular hybrid combination.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002051:Burkitt Lymphoma; D002459:Cell Fusion; D002875:Chromosomes; D002999:Clone Cells; D004279:DNA, Viral / Q000032:analysis*; D004854:Herpesvirus 4, Human / Q000276:immunology*; D006801:Humans; D006822:Hybrid Cells / Q000201:enzymology / Q000648:ultrastructure; D007527:Isoenzymes / Q000032:analysis*; D008325:Mammary Neoplasms, Experimental; D051379:Mice; D009693:Nucleic Acid Hybridization
D000956:Antigens, Viral; D004279:DNA, Viral; D007527:Isoenzymes