DNA unwinding protein from meiotic cells of Lilium.
An ATP-dependent DNA unwinding protein is present at a high level of activity in meiotic cells of lilies. The protein also acts as a DNA-dependent ATPase, the single strand form being the preferred cofactor. It binds in the absence of ATP to single-strand DNA and to ends or nicks in duplex DNA. A 3'-OH terminus is required for binding at duplex ends; such binding is highly stable. Unwinding occurs in the presence of ATP, and it is limited to about 50 base pairs per end or 400-500 base pairs per nick. The ATP hydrolyzed during unwinding is distinguishable from ATP hydrolysis in the presence of single-strand DNA.
D000251:Adenosine Triphosphatases / Q000378:metabolism; D004247:DNA; D004265:DNA Helicases / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D008540:Meiosis; D018521:Plant Physiological Phenomena; D010944:Plants / Q000201:enzymology*; D011485:Protein Binding
D004247:DNA; D000251:Adenosine Triphosphatases; D004265:DNA Helicases