title: The frequencies of transcription from the E- and L-strands of polyoma DNA.
abstract: Exhaustion type hybridization was used to measure the amount of nuclear virus RNA complementary to the early (E) and late (L) polyoma virus DNA strands. At 36 h after infection between 2.5 and 7.3% of the newly synthesized virus RNA was complementary to the E-strand (-strand) and between 92.7 and 97.5% was complementary to the L-strand (+strand). This proportion was independent of the labelling time, indicating similar accumulation of the E- and L-RNA transcripts in the nucleus. The nuclear E- and L-RNA transcripts sedimented in a similar manner through sucrose gradients.
mesh_terms: D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D004279:DNA, Viral / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D011120:Polyomavirus / Q000235:genetics* / Q000378:metabolism; D012367:RNA, Viral / Q000096:biosynthesis; D014158:Transcription, Genetic
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral