title: Chirality of the Hydrogen transfer to NAD catalyzed by (3R) hydroxybutyrate dehydrogenase from Pseudomonas lemoignei.
abstract: The chirality of the hydrogen transfer from (3R) hydroxybutyrate to NAD catalyzed by (3R)hydroxybutyrate dehydrogenase (E.C. 1.1.1.30, D-3-hydroxybutyrate : NAD oxidoreductase) from Pseudomonas lemoignei was investigated. [4(-3)H] NAD was enzymatically reduced to (4R) [4(-3)H]NADH with (3RS) hydroxybutyrate. This observation was confirmed since NAD could be reduced to (4S) [4(-3)H] NADH with (3RS) [3(-3)H] hydroxybutyrate and (3R) hydroxybutyrate dehydrogenase. From these experiments it can be concluded that (3R) hydroxybutyrate dehydrogenase from P. lemoignei should be classified as an B or (S) type dehydrogenase.
mesh_terms: D006884:Hydroxybutyrate Dehydrogenase / Q000378:metabolism*; D009243:NAD; D011549:Pseudomonas / Q000201:enzymology*; D013237:Stereoisomerism; D013329:Structure-Activity Relationship; D014316:Tritium
chemical_list: D009243:NAD; D014316:Tritium; D006884:Hydroxybutyrate Dehydrogenase