[Effect on some pyridine derivatives on the activity of heart NAD glycohydrolase].
Effect of some 3- and 4-substituted pyridines on enzymatic hydrolysis of NAD by rabbit heart muscle NAD-glycohydrolase has been studied. It is shown that some 4-substituted derivatives in contrast with 3-substituted ones produce an inhibitory effect on the enzyme activity. A new efficient inhibitor of rabbit heart muscle NAD-glycohydrolase (I50 = 10(-3) M)--N1-(2-lactyl)-N2-(isonicotinoy)hydrazine, inducing uncompetitive inhibition of hydrolysis of NAD is found. The mechanism of the inhibitory effect of N1-(2-lactyl)-N2-(isonicotinoyl)hydrazine was investigated and the rate equation for enzymatic hydrolysis of NAD in the presence of inhibitor is calculated. It is suggested, that the inhibitory effect of N1-(2-lactyl)-N2-(isonicotinoyl)hydrazine is due to the formation of triple inactive complex inhibitor-enzyme-adenosinediphosphateribose.
D000247:Adenosine Diphosphate Sugars / Q000378:metabolism; D000818:Animals; D006868:Hydrolysis; D007538:Isoniazid / Q000031:analogs & derivatives / Q000494:pharmacology; D009206:Myocardium / Q000201:enzymology*; D009244:NAD+ Nucleosidase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011725:Pyridines / Q000494:pharmacology*; D011817:Rabbits; D013329:Structure-Activity Relationship
D000247:Adenosine Diphosphate Sugars; D011725:Pyridines; D009244:NAD+ Nucleosidase; D007538:Isoniazid
Vliianie nekotorykh proisvodnykh piridina na aktivnost'NAD-glikogidrolazy iz serdechnoi myshtsy.