Evidence that latent collagenases are enzyme-inhibitor complexes.
Specific collagenase from the culture media of various rabbit tissues and cells exists in active and latent forms. Latent collagenase is most effectively activated with 4-aminophenylmercuric acetate, a thiol-blocking reagent, strongly suggesting that latent forms are enzyme-inhibitor complexes. A collagenase inhibitor from bone cultures, which may be closely related to the inhibitor of such latent enzyme complexes, was partially characterized.
D000818:Animals; D001842:Bone and Bones / Q000201:enzymology; D004789:Enzyme Activation; D066298:In Vitro Techniques; D003012:Microbial Collagenase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D008970:Molecular Weight; D010662:Phenylmercuric Acetate / Q000031:analogs & derivatives; D011817:Rabbits; D014357:Trypsin
D014357:Trypsin; D003012:Microbial Collagenase; D010662:Phenylmercuric Acetate