Cholinergic metabolism and synapse formation by a rat nerve cell line.
The PC12 clone of a rat pheochromocytoma is able to synthesize acetylcholine. The amount of acetylcholine synthesized, and the specific activity of choline O-acetyltransferase (acetyl-CoA:choline O-acetyltransferase, EC 2.3.1.6), varies as a function of the culture growth curve and is dependent on cell density. The specific activity of choline acetyltransferase is increased by nerve growth factor, growth-conditioned medium from a variety of cell types, and adenosine 3':5'-monophosphate. Finally, the PC12 cell line is able to form cholinergic synapses with a clonal cell line of skeletal muscle origin.
D000109:Acetylcholine / Q000096:biosynthesis*; D000123:Acetyltransferases / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D002795:Choline O-Acetyltransferase / Q000378:metabolism*; D000242:Cyclic AMP / Q000494:pharmacology; D007700:Kinetics; D009414:Nerve Growth Factors / Q000494:pharmacology; D009928:Organ Specificity; D010673:Pheochromocytoma / Q000378:metabolism*; D051381:Rats; D013569:Synapses / Q000187:drug effects / Q000502:physiology*; D013806:Theophylline / Q000494:pharmacology
D009414:Nerve Growth Factors; D013806:Theophylline; D000242:Cyclic AMP; D000123:Acetyltransferases; D002795:Choline O-Acetyltransferase; D000109:Acetylcholine