title: Low molecular weight angiotensin I converting enzyme from rat lung.
abstract: A low molecular weight angiotensin I converting enzyme (light angiotensin enzyme) was isolated from a homogenate of rat lung subjected to dialysis against sodium acetate at pH 4.8. This enzyme has a molecular weight of 84 000 on Sephadex G-200 and a molecular weight of 91 000 on SDS-poly-acrylamide gel as compared with a molecular weight of 139 000 for angiotensin I converting enzyme on SDS-polyacrylamide. Light angiotensin enzyme was activated by NaCl  and inhibited by EDTA, angiotensin II, and bradykinin potentiating factor nonapeptide. Light angiotensin enzyme cross-reacted with antibody prepared against angiotensin I converting enzyme and stained with periodic acid-Schiff reagent as a glycoprotein. The evidence suggests that light angiotensin enzyme is a fragment of the higher molecular weight enzyme.
mesh_terms: D000818:Animals; D004492:Edetic Acid / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D008168:Lung / Q000201:enzymology*; D046911:Macromolecular Substances; D008970:Molecular Weight; D009842:Oligopeptides / Q000494:pharmacology; D007703:Peptidyl-Dipeptidase A / Q000302:isolation & purification / Q000378:metabolism; D051381:Rats; D012965:Sodium Chloride / Q000494:pharmacology
chemical_list: D046911:Macromolecular Substances; D009842:Oligopeptides; D012965:Sodium Chloride; D004492:Edetic Acid; D007703:Peptidyl-Dipeptidase A