Release of periplasmic enzymes from Escherichia coli by penicillin-ethylenediaminetetraacetate treatment.
When Escherichia coli cells are converted into spheroplasts by penicillin treatment, none of the periplasmic enzymes is released. However, incubation of the penicillin-induced spheroplasts in a medium containing ethylenediaminetetraacetic acid caused the release of endonuclease I and cyclic phosphodiesterase but not of beta-galactosidase.
D004492:Edetic Acid / Q000494:pharmacology*; D004720:Endonucleases / Q000378:metabolism*; D004926:Escherichia coli / Q000187:drug effects* / Q000201:enzymology; D005696:Galactosidases / Q000378:metabolism; D010406:Penicillins / Q000494:pharmacology*; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D013104:Spheroplasts / Q000187:drug effects* / Q000201:enzymology
D010406:Penicillins; D004492:Edetic Acid; D004720:Endonucleases; D010727:Phosphoric Diester Hydrolases; D005696:Galactosidases