Genetics of resistance to phosphonoacetic acid in strain KOS of herpes simplex virus type 1.
A DNA- temperature-sensitive mutant of herpes simplex virus type 1 exhibiting thermolabile DNA polymerase activity, tsD9, was shown to be resistant to phosphonoacetic acid (PAA) when plated at the permissive temperature. ts+ revertants of tsD9 were PAA sensitive and exhibited DNA polymerase activity intermediate between that of the wild-type virus and tsD9, indicating that both temperature sensitivity and sensitivity to PAA are controlled by the same gene. Since the position of tsD9 on the existing herpes simplex virus type 1 linkage map is known, the locus for PAA resistance--and therefore for the structural gene for viral DNA polymerase--has been identified.
D000085:Acetates / Q000494:pharmacology*; D000998:Antiviral Agents / Q000494:pharmacology*; D004259:DNA-Directed DNA Polymerase / Q000096:biosynthesis; D004352:Drug Resistance, Microbial; D005796:Genes; D008040:Genetic Linkage; D009154:Mutation; D009943:Organophosphorus Compounds / Q000494:pharmacology*; D018139:Simplexvirus / Q000187:drug effects* / Q000201:enzymology; D013696:Temperature
D000085:Acetates; D000998:Antiviral Agents; D009943:Organophosphorus Compounds; D004259:DNA-Directed DNA Polymerase