Separation of a glycoprotein antigen for the diagnosis of bovine leukemia and some of its biochemical properties.
Data are presented on the separation and on some biochemical properties of an antigen to be used in the immunodiffusion (ID) test for detection of bovine leukemia virus. The antigen obtained proved to have the same efficiency in the ID test as a commercially available standard antigen. Experimental results suggest that the antigen is released into the culture fluid in the form of a glycoprotein molecule consisting of two subunits with a molecular weight of 60,000 linked by--SH bonds.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000276:immunology; D005779:Immunodiffusion; D007938:Leukemia / Q000175:diagnosis*; D001909:Leukemia Virus, Bovine / Q000276:immunology*; D012190:Retroviridae / Q000276:immunology*; D014764:Viral Proteins / Q000276:immunology
D000956:Antigens, Viral; D006023:Glycoproteins; D014764:Viral Proteins