Effect of decentralization and glucose withdrawal on the potassium-induced cAMP increase in the rabbit superior cervical ganglion.
The concentration of cAMP in the isolated rabbit superior cervical ganglion (SCG) increases when the tissue is exposed to high potassium media for short periods. The increase was found not to be affected by agents that are known to potentiate or to antagonize the postsynaptic effects of acetylcholine. Pretreatment of the rabbits with various sympaticolytic substances did not appear to affect the cAMP increase in the isolated SCG. It was found that the K+-induced cAMP synthesis occurred neither in ganglia which had been decentralized 3 days before isolation nor in intact ganglia preincubated in a glucose-free medium. Under both these conditions the effect of potassium declines with a time course which is characteristic for the degeneration of the presynaptic terminals. These terminals therefore, appear to be the most likely site for the cAMP increase.
D000818:Animals; D000242:Cyclic AMP / Q000096:biosynthesis*; D005725:Ganglia, Autonomic / Q000187:drug effects* / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D009410:Nerve Degeneration / Q000187:drug effects; D011188:Potassium / Q000494:pharmacology*; D011817:Rabbits; D013806:Theophylline / Q000494:pharmacology
D013806:Theophylline; D000242:Cyclic AMP; D005947:Glucose; D011188:Potassium