Uptake of alpha-aminoisobutyric acid and phosphate by membrane vesicles derived from growing and quiescent fibroblasts.
Membrane vesicles derived principally from the plasma membrane and endoplasmic reticulum of mouse 3T3 cells transformed by Simian virus 40 take up alpha-aminoisobutyric acid (AIB) and phosphate (Pi). When NaCl is added simultaneously with AIB or Pi, uptake rises two- to three-times above the equilibrium to accumulate AIB or Pi over the control value, in the presence of a Na+ gradient, is almost lost in membrane vesicles derived from benzpyrene-transformed 3T3 cells (BP3T3) arrested in the G1 phase of the cell cycle by serum starvation. When added to the membranes with NaCl and the uptake substrate, a combination of fibroblast growth factor (FGF) and epidermal growth factor EGF restores the ability of the membranes to accumulate AIB and Pi over the control value.
D000621:Aminoisobutyric Acids / Q000378:metabolism*; D000818:Animals; D001580:Benzopyrenes; D001693:Biological Transport, Active / Q000187:drug effects; D002455:Cell Division; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002471:Cell Transformation, Neoplastic; D004721:Endoplasmic Reticulum / Q000187:drug effects / Q000378:metabolism; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D006133:Growth Substances / Q000494:pharmacology; D051379:Mice; D010710:Phosphates / Q000378:metabolism*; D013539:Simian virus 40; D012965:Sodium Chloride / Q000494:pharmacology
D000621:Aminoisobutyric Acids; D001580:Benzopyrenes; D006133:Growth Substances; D010710:Phosphates; D012965:Sodium Chloride; D004815:Epidermal Growth Factor