title: Differences between the structural dynamics of plasma membranes of normal hamster lymphocytes and lymphoid cells neoplastically transformed by simian virus 40 as revealed by laser Raman spectroscopy.
abstract: The Raman spectra of highly purified plasma membranes from SV40-transformed GD248 lymphocytes have been compared with the spectra of the membranes of normal cells over the spectral region 100 cm-1 to 3010 cm-1. Striking differences between the two membrane categories were observed in the thermal response of the CH-stretching and acoustical regions. Analysis of CH-stretching shows that the membranes of normal cells exhibit a thermal transition centered at 7 degrees and approximately 5 degrees wide. The membranes of GD248 cells, in contrast, show a lipid transition centered at -5 degrees and 12-18 degrees wide. Analysis of the acoustical region yields equivalent results. The membrane proteins of normal membranes undergo a large thermotropic transition, starting at 39 degrees (sample temperatures), whereas this transition begins at 23 degrees with GD248 plasma membranes. The results suggest the possibility that SV40-specific membrane proteins may modify the collective thermotropic behavior of both normal membrane proteins and membrane lipids.
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000648:ultrastructure*; D002471:Cell Transformation, Neoplastic; D055598:Chemical Phenomena; D002621:Chemistry; D002784:Cholesterol; D006224:Cricetinae; D007834:Lasers; D008214:Lymphocytes / Q000648:ultrastructure*; D008563:Membrane Lipids; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D013539:Simian virus 40; D013059:Spectrum Analysis, Raman; D013696:Temperature; D013816:Thermodynamics
chemical_list: D008563:Membrane Lipids; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D002784:Cholesterol