Establishment and characterization of a cell line derived from a spontaneous murine lung carcinoma (M109).
The Madison lung (M109) tumor cell line, initiated from a "spontaneous", anaplastic murine lung carcinoma, has been propagated continuously in vitro for more than 300 cell generations. Cytogenetic analysis revealed a mouse karyotype with a mode of 78 chromosomes (2n = 40). Three distinct marker chromosomes were identified by trypsin-giemsa banding. The cells piled up in culture and had a short generation time and high plating efficiency. Electron microscopy revealed highly undifferentiated cells with little rough endoplasmic reticulum, an abundance of free polysomes, the presence of few and often odd-shaped mitochondria, lipid bodies and phagocytic vacuoles. Virus particles of the C-type were found frequently. The subcutaneous transplantation of M109 cultured cells at a relatively low cell inoculum produced highly metastatic tumors in syngeneic BALG/c mice.
D002282:Adenocarcinoma, Bronchiolo-Alveolar / Q000648:ultrastructure; D000818:Animals; D002455:Cell Division; D002460:Cell Line; D002462:Cell Membrane / Q000382:microbiology; D002467:Cell Nucleus / Q000648:ultrastructure; D002875:Chromosomes / Q000032:analysis; D003593:Cytoplasm / Q000382:microbiology / Q000648:ultrastructure; D008175:Lung Neoplasms / Q000648:ultrastructure; D051379:Mice; D008807:Mice, Inbred BALB C; D009374:Neoplasms, Experimental; D009940:Organoids / Q000648:ultrastructure; D012190:Retroviridae / Q000254:growth & development