Kinetics of rac-1-oleyl-2-[4-(3-pyrenyl)butanoyl]glycerol transfer between high density lipoproteins.
The mechanism of transfer of diglyceride between high density lipoproteins (HDL) was investigated with a pyrene-containing analogue whose fluorescent properties depend on the microscopic concentration in the lipoprotein. Transfer rates were first order, rapid (3.5s-1), and invariant over a 100-fold range of HDL concentration and over a 10-fold range of rac-1-oleyl-2-[4-(3-pyrenyl)butanoyl]glycerol concentrations. Similar behavior of the probe was observed with HDL3, although the rate was 40% slower. These results support a mechanism in which rate-limiting dissociation of the diglyceride analogue from one HDL particle into the aqueous phase precedes rapid diffusion and subsequent uptake by another such particle.
D004075:Diglycerides; D005989:Glycerides; D006801:Humans; D007700:Kinetics; D008075:Lipoproteins, HDL / Q000097:blood; D008968:Molecular Conformation; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics
D004075:Diglycerides; D005989:Glycerides; D008075:Lipoproteins, HDL