Arylsulfonyltetrazoles, new coupling reagents and further improvements in the triester method for the synthesis of deoxyribooligonucleotides.
The modified triester approach has been further improved and refined to the synthesis of defined sequences of deoxyribo-oligonucleotides. Improvements include arylsulfonyltetrazoles as faster and milder condensing agents, benzenesulfonic acid to avoid depurination during deblocking of trityl protecting groups and improved chromatographic procedures for purification of triester intermediates and purification of the final product containing 3'-5' phosphodiester linkages.
D004262:DNA Restriction Enzymes; D007202:Indicators and Reagents; D008722:Methods; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000302:isolation & purification; D009841:Oligonucleotides / Q000138:chemical synthesis*; D010727:Phosphoric Diester Hydrolases; D013777:Tetrazoles
D007202:Indicators and Reagents; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D013777:Tetrazoles; D004262:DNA Restriction Enzymes; D010727:Phosphoric Diester Hydrolases