Enzymatic digestion of DNA gamma-irradiated in aqueous solution separation of the digests by ion-exchange chromatography.
Aqueous solutions of DNA were gamma-irradiated in the presence and absence of oxygen and enzymatically hydrolysed by the combined action of pancreatic deoxyribonuclease (DNase I), snake-venom phosphodiesterase (PDE I), spleen phosphodiesterase (PDE II) and alkaline phosphatase. In contrast to unirradiated DNA, which is fully hydrolysed to nucleosides by these enzymes, gamma-irradiated DNA yields a series of oligonucleotides. Their isolation might enalbe the future identification of the chemical nature of DNA lesions.
D000469:Alkaline Phosphatase; D002852:Chromatography, Ion Exchange; D004247:DNA / Q000528:radiation effects*; D003851:Deoxyribonucleases; D005720:Gamma Rays; D010727:Phosphoric Diester Hydrolases
D004247:DNA; D003851:Deoxyribonucleases; D000469:Alkaline Phosphatase; D010727:Phosphoric Diester Hydrolases