title: The binding site on SV40 DNA for a T antigen-related protein.
abstract: A protein closely related to SV40 T antigen was purified in a biologically active form from cells infected with the defective adenovirus-SV40 hybrid, Ad2+D2. This 107,000 dalton hybrid protein binds and protects a specific portion of SV40 DNA from digestion by pancreatic DNAase I. Hybridization, endonuclease cleavage and pyrimidine tract analysis of the protected fragments reveal that the D2 hybrid protein binds in a sequential manner to tandem recognition sites which lie within a sequence of 120 nucleotides at position 67 near the origin of SV40 replication.
mesh_terms: D000256:Adenoviridae; D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D001483:Base Sequence; D001665:Binding Sites; D004261:DNA Replication; D004279:DNA, Viral / Q000378:metabolism*; D003673:Defective Viruses; D003851:Deoxyribonucleases / Q000378:metabolism; D013539:Simian virus 40 / Q000276:immunology; D014764:Viral Proteins / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D004279:DNA, Viral; D014764:Viral Proteins; D003851:Deoxyribonucleases