Bactericidal action of ascorbic acid on Psuedomonas aeruginosa: alteration of cell surface as a possible mechanism.
Neutralised ascorbic acid is found to exert a strong bactericidal action on Pseudomonas aeruginosa suspended in isotonic phosphate buffer at pH 7.1. Both the bactericidal and bacteriostatic action of ascorbic acid are antagonised by magnesium ions. In the absence of complex formation between magnesium and ascorbic acid it is concluded that ascorbic acid acts by competing with the magnesium binding sites in the cell wall, cell membrane or ribosomes. Using the chequer-board titration method the synergistic action of ascorbic acid and erythromycin is determined; such a potentiation of erythromycin is also adversely affected by magnesium ions. P. aeruginosa cells, washed and suspended in isotonic phosphate buffer containing ascorbic acid, became increasingly susceptible to the action of polymyxin, erythromycin, chloramphenicol, neomycin and tetracycline. It is suggested that ascorbic acid alters the cell surface to render it increasingly permeable to these antibiotics.
D001205:Ascorbic Acid / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000494:pharmacology*; D002463:Cell Membrane Permeability / Q000187:drug effects*; D002701:Chloramphenicol / Q000494:pharmacology; D004357:Drug Synergism; D004917:Erythromycin / Q000494:pharmacology; D008274:Magnesium / Q000378:metabolism / Q000494:pharmacology; D009355:Neomycin / Q000494:pharmacology; D011113:Polymyxins / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D013752:Tetracycline / Q000494:pharmacology
D011113:Polymyxins; D004917:Erythromycin; D002701:Chloramphenicol; D013752:Tetracycline; D009355:Neomycin; D008274:Magnesium; D001205:Ascorbic Acid