Effects of guanidine hydrochloride on human plasma high density lipoproteins.
Denaturation of human plasma high density lipoproteins during ultracentrifugation in guanidine-HCl is characterized by: dissociation of apoA-I, in the range of 2-3 M guanidine-HCl, and dissociation of apoA-I and apoA-II in 5-6 M guanidine-HCl. Denaturation of high density lipoprotein species, during a sequence of timed exposure to guanidine-HCl followed first by removal of the denaturant by dialysis and then by ultracentrifugation, is characterized by:dissociation of lipid-poor apoA-I, which follows a time course similar to denaturation-related changes in reported spectroscopic parameters; and apparent formation of lipoprotein aggregation products depleted in apoA-I and relatively enriched in apoA-II. These studies indicate differential properties of the major apoproteins in stabilizing high density lipoprotein structure and characterize a mode of lipoprotein transformation and degradation which apparently results from apoprotein dissociation coupled with aggregation of denatured lipoprote species.
D000328:Adult; D001053:Apolipoproteins / Q000097:blood; D001665:Binding Sites; D005260:Female; D006146:Guanidines; D006801:Humans; D007700:Kinetics; D008075:Lipoproteins, HDL / Q000097:blood; D008854:Microscopy, Electron; D008875:Middle Aged; D008970:Molecular Weight; D011485:Protein Binding; D011487:Protein Conformation; D011489:Protein Denaturation; D014461:Ultracentrifugation
D001053:Apolipoproteins; D006146:Guanidines; D008075:Lipoproteins, HDL