Regulation of purine biosynthesis in cultured Drosophila melanogaster cells: I.--Conditional activity of hypoxanthine-guanine-phosphoribosyltransferase and 5-nucleotidase.
In cultured cells established from Drosophila melanogaster embryos, and grown in usual medium, no hypoxanthine-guanine-phosphoribosyl transferase (HG-PRT) could be measured, and only traces of 5'-nucleotidase activity were detectable. On the contrary, it was observed that if the same medium is supplemented with purine bases, nucleosides, orotate, glutamine, azaserine or antifolates, de novo purine biosynthesis is inhibited, and HGPRT is detectable, along with an important 5'-nucleotidase activity. Moreover, dialysis or treatment of extracts from cells untreated by purines, with activated charcoal restored HGPRT and 5'-nucleotidase activities. These activities were abolished completely by inosinic acid (IMP) and guanosine 5'-monophosphoric acid (GMP). Similar results were obtained with fly extracts. These results suggest that de novo purine biosynthesis masks HGPRT activity, the endogenous synthesis leading to the accumulation of purine nucleotides which are inhibitors of the HGPRT activity.
D000818:Animals; D002457:Cell Extracts / Q000494:pharmacology; D002478:Cells, Cultured; D004331:Drosophila melanogaster / Q000201:enzymology*; D006157:Guanosine Monophosphate / Q000494:pharmacology; D007041:Hypoxanthine Phosphoribosyltransferase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D007291:Inosine Monophosphate / Q000494:pharmacology; D009708:Nucleotidases / Q000378:metabolism*; D011687:Purines / Q000096:biosynthesis* / Q000494:pharmacology
D002457:Cell Extracts; D011687:Purines; D007291:Inosine Monophosphate; D006157:Guanosine Monophosphate; D007041:Hypoxanthine Phosphoribosyltransferase; D009708:Nucleotidases