AKR mouse leukemic cells in tissue culture. II. Immunologic studies on the AKR 87 cell line.
The continuous AKR 87 cell line was derived from the thymus and spleen of AKR mice with spontaneous leukemia. Previous studies showed that the line has retained the original morphologic features of leukemic cells, reacts positively in the XC test, and produces virus particles of type C morphology. The present paper presents an immunologic characterization of cells of the AKR 87 line and of the virus produced by them, in comparison with the cells of spontaneous leukemia of AKR mice, TGV line productively infected with Gross virus, and normal mouse tissues. Results of the hemagglutination test, immunoprecipitation in agar gel, cytotoxic test and immunofluorescence indicate that the AKR 87 line produces virus antigen of the Gross type.
D000406:AKR murine leukemia virus / Q000276:immunology*; D000818:Animals; D000956:Antigens, Viral / Q000302:isolation & purification; D002460:Cell Line; D002474:Cell-Free System; D003470:Culture Media; D003601:Cytotoxicity Tests, Immunologic; D005455:Fluorescent Antibody Technique; D006386:Hemagglutination Tests; D009052:Leukemia Virus, Murine / Q000276:immunology*; D007942:Leukemia, Experimental / Q000276:immunology*; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008811:Mice, Inbred DBA; D014412:Tumor Virus Infections / Q000276:immunology*
D000956:Antigens, Viral; D003470:Culture Media