title: Activation of cAMP-dependent protein kinase without a corresponding increase in phosphorylase activity.
abstract: The effect of prostaglandin E1(PGE1) and epinephrine on glycogen metabolism has been investigated in the perfused rat heart. Both agents produced increases in cAMP and the cAMP-dependent protein kinase activity ratio. When dosages were adjusted to give equal increases in the protein kinase activity ratio from a basal value of 0.15 to as high as 0.40, only epinephrine caused a significant increase in phosphorylase activity. When used together, PGE1 did not effect the ability of epinephrine to increase phosphorylase activity.
mesh_terms: D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D004837:Epinephrine / Q000494:pharmacology; D066298:In Vitro Techniques; D009206:Myocardium / Q000201:enzymology; D006005:Phosphorylases / Q000378:metabolism*; D011458:Prostaglandins E / Q000494:pharmacology; D011494:Protein Kinases / Q000378:metabolism*; D051381:Rats
chemical_list: D011458:Prostaglandins E; D000242:Cyclic AMP; D006005:Phosphorylases; D011494:Protein Kinases; D004837:Epinephrine