Adenylate cyclase and phosphodiesterase activity in rabbit ureter.
Adenylate cyclase and phosphodiesterase enzyme activities were demonstrated in rabbit ureter. NaF, 10 mM, caused a 60.9 per cent increase in adenylate cyclase activity. Isoproterenol, 5 X 10-7 to 10-5 M induced a statistically significant dose-dependent increase in adenylate cyclase activity which was suppressed by propranolol, 10-7 M. Theophylline, 5 X 10-5 to 10-2 M, significantly inhibited phosphodiesterase activity. Thus, isoproterenol and theophylline, two agents that can relax ureteral segments previously contracted by a depolarizing concentration of potassium, could presumably increase cyclic AMP levels, isoproterenol by increasing synthesis and theophylline by decreasing degradation.
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D001341:Autonomic Nervous System / Q000502:physiology; D007545:Isoproterenol / Q000494:pharmacology; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D011433:Propranolol / Q000494:pharmacology; D011817:Rabbits; D012969:Sodium Fluoride / Q000494:pharmacology; D013806:Theophylline / Q000494:pharmacology; D014513:Ureter / Q000201:enzymology*
D012969:Sodium Fluoride; D011433:Propranolol; D013806:Theophylline; D010727:Phosphoric Diester Hydrolases; D000262:Adenylyl Cyclases; D007545:Isoproterenol