Evidence for the involvement of superoxide in vitamin K-dependent carboxylation of glutamic acid residues of prothrombin.
The formation of vitamin K epoxide and the vitamin K-dependent carboxylation of glutamic acid residues present in synthetic substrates and decarboxyprothrombin are both inhibited by superoxide dismutase. Catalase only inhibits the generation of vitamin K epoxide, suggesting that the carboxylation and epoxidation reactions are not inter-dependent.
D000818:Animals; D002374:Catalase / Q000494:pharmacology; D055598:Chemical Phenomena; D002621:Chemistry; D005971:Glutamates / Q000378:metabolism*; D066298:In Vitro Techniques; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D010100:Oxygen / Q000096:biosynthesis*; D011516:Prothrombin / Q000378:metabolism*; D051381:Rats; D013482:Superoxide Dismutase / Q000494:pharmacology; D013481:Superoxides / Q000096:biosynthesis*; D014812:Vitamin K / Q000378:metabolism*
D005971:Glutamates; D013481:Superoxides; D014812:Vitamin K; D011516:Prothrombin; D002374:Catalase; D013482:Superoxide Dismutase; D010100:Oxygen