Stable transformation of mouse, rabbit and monkey cells and abortive transformation of human cells by BK virus, a human papovavirus.
Semi-permissive mouse, rabbit and monkey cells were stably transformed by BK virus (BKV). The specificity of transformation was demonstrated by the presence of BKV tumour (T) antigen in nuclei of transformed cells and by virus rescue with Sendai virus-mediated fusion or transfection. Two out of seven BKV-transformed cell lines were oncogenic. Permissive human cells were only abortively transformed by BKV, since morphologically modified cells persisted in culture for a few passages and eventually died.
D000818:Animals; D000951:Antigens, Neoplasm; D001739:BK Virus / Q000235:genetics*; D002460:Cell Line; D002472:Cell Transformation, Viral; D003260:Contact Inhibition; D003588:Cytopathogenic Effect, Viral; D004279:DNA, Viral; D005347:Fibroblasts; D000882:Haplorhini; D006801:Humans; D007668:Kidney / Q000166:cytology; D051379:Mice; D011120:Polyomavirus / Q000235:genetics*; D011817:Rabbits; D014162:Transfection
D000951:Antigens, Neoplasm; D004279:DNA, Viral