[Influence of phospholipids of different natures on the structure of ferricytochrome c].
Influence of the two types of lipid molecules (neutral and acidic phospholipids) on the structure of ferricytochrome c in lipid-protein complexes was studied with the use of circular dichroism and absorption spectroscopy methods. It was found that interaction of ferricytochrome c with acidic phospholipids (cardiolipin and phosphatidylinositols) brings about some changes in the protein conformation, while interaction with neutral phospholipid (phosphatidylcholine plus 10% lauric acid) does not. Some difference in the mode of interaction of different acidic phospholipids with ferricytochrome c was also observed. As evidenced by visual light absorption spectra, cardiolipin induced the disruption of hem--methionine 80 bond in the protein molecule, while phosphatidylinositols do not cause such an effect.
D002308:Cardiolipins; D002942:Circular Dichroism; D003574:Cytochrome c Group; D010713:Phosphatidylcholines; D010716:Phosphatidylinositols; D010743:Phospholipids; D011487:Protein Conformation; D013057:Spectrum Analysis
D002308:Cardiolipins; D003574:Cytochrome c Group; D010713:Phosphatidylcholines; D010716:Phosphatidylinositols; D010743:Phospholipids
Vliianie fosfolipidov razlichnoĭ prirody na strukturu ferritsitokhroma c.