Characterization of a unique cell line (LAZ 221) from human acute lymphocytic ("null" cell) leukemia.
A unique human cell line designated LAZ 221 has been established from the peripheral blood of a patient with acute lymphocytic leukemia of the "null" cell type. The cell line does not possess the Epstein-Barr virus nuclear antigen and has a karyotype of 45,XX,-9,-12,+(9q12q). Both the established cell line and the patient's uncultured blast cells share the same phenotypic markers. They both lack T-cell markers. They fail to form sheep erythrocyte rosettes and do not react with T-cell-specific antisera (TH1-, HTL-), nor do they possess B-cell markers. They do not form rosettes with erythrocytes sensitized with complement, and they are surface immunoglobulin negative. However, they do possess an HLA-D-related glycoprotein complex of 23,000 to 30,000 daltons, an la-like antigen. Thus, LAZ 221 shares the phenotype of the patient's uncultured blasts and is a cell line representative of about 75% of all human acute lymphocytic leukemias. In this respect it differs from previously described human hematopoietic cell lines.
D000328:Adult; D000951:Antigens, Neoplasm; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D002869:Chromosome Aberrations; D002906:Chromosomes, Human, 6-12 and X; D005260:Female; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D007519:Isoantigens; D007945:Leukemia, Lymphoid / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D008214:Lymphocytes / Q000276:immunology*; D009374:Neoplasms, Experimental / Q000276:immunology
D000951:Antigens, Neoplasm; D007519:Isoantigens