[Effect of ions on determination of 3',5'-AMP by the method of saturation].
The effects of La2+, Pb2+, Ba2+, Zn2+, Ca2+, Mg2+, Mn2+, NH+4 in concentrations of 10(-7), 10(-5), 10(-3) M were studied as applied to the protein binding of cyclic 3' : 5' = AMP. It is shown that Ba2+, La2+, Pb2+ increase the binding, while Zn2+ has no effect, and Mn2+, NH+4 decrease the binding of 3' : 5' = AMP. The effects of Ca2+ and Cd2+ were dependent on the concentration. The maximal binding in the presence of Ca2+ and Cd2+ was observed at a concentration of 10(-5) M and 10(-3) M, respectively. It is suggested that Mg2+ and Ca2+ take part in the regulation of cyclic AMP binding with proteinkinase in vivo.
D001464:Barium / Q000494:pharmacology; D002104:Cadmium / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D002413:Cations, Divalent / Q000494:pharmacology*; D000242:Cyclic AMP / Q000032:analysis*; D007811:Lanthanum / Q000494:pharmacology; D007854:Lead / Q000494:pharmacology; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D011485:Protein Binding; D011494:Protein Kinases / Q000378:metabolism; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology
D002413:Cations, Divalent; D000644:Quaternary Ammonium Compounds; D002104:Cadmium; D001464:Barium; D007854:Lead; D008345:Manganese; D007811:Lanthanum; D000242:Cyclic AMP; D011494:Protein Kinases; D008274:Magnesium; D002118:Calcium
Bliianie ionov na opredelenie 3':5'-AMP metodom nasyshcheniia.