Use of isolated nuclei in the indirect fluorescent-antibody test for human cytomegalovirus infection: comparison with microneutralization, anticomplement, and conventional indirect fluorescent-antibody assays.
Use of an antigen consisting of purified isolated nuclei from a mixture of human cytomegalovirus-infected and uninfected fibroblasts in a 2:1 ratio is a simple and reliable method for eliminating nonspecific fluorescence associated with the presence of Fc-immunoglobulin G receptors in the cytoplasm of infected cells. The specificity obtained with this antigen on 100 normal human sera was 99, 100, and 98% when compared with microneutralization, anticomplement immunofluorescence, and conventional indirect fluorescent-antibody assays, respectively. Also, 95% of the antibody titers obtained with the nuclear antigen had a perfect correlation with or were within a fourfold-dilution difference of the antibody levels obtained by anticomplement immunofluorescence and the conventional indirect fluorescent-antibody test.
D000914:Antibodies, Viral / Q000032:analysis; D000956:Antigens, Viral; D002467:Cell Nucleus / Q000276:immunology; D003168:Complement Fixation Tests; D046508:Culture Techniques; D003586:Cytomegalovirus Infections / Q000175:diagnosis*; D003937:Diagnosis, Differential; D005260:Female; D005347:Fibroblasts; D005455:Fluorescent Antibody Technique / Q000379:methods*; D006801:Humans; D009500:Neutralization Tests; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000175:diagnosis*
D000914:Antibodies, Viral; D000956:Antigens, Viral