title: Membranous localization and properties of ATPase of rat liver lysosomes.
abstract: Lysosomes isolated from rat liver were found to have ATPase activity (EC No 3.6.1.3). Subfractionation of the lysosomes revealed a membranous localization of ATPase activity. The enzyme has half maximal activity at 0.2 mM ATP and is inhibited by high concentrations of ATP. The apparent Km for divalent metal is 0.2 mM, and either Ca2+ or Mg2+ give maximal activity. The ATPase activity has latency when lysosomes are isolated from rats treated with Triton WR-1339. This latency may be due to the presence of internalized sucrose because the activity of L fraction lysosomes is much less latent and Triton WR-1339 itself is not inhibitory. The latency of glucosaminidase, a marker enzyme for lysosomes, contrasts with the low latency of the ATPase and points to an ATPase with an exposed active site in intact lysosomes.
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002414:Cations, Monovalent / Q000494:pharmacology; D002462:Cell Membrane / Q000201:enzymology; D003576:Electron Transport Complex IV / Q000378:metabolism; D006596:Hexosaminidases / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D008247:Lysosomes / Q000201:enzymology*; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D008566:Membranes / Q000201:enzymology*; D008930:Mitochondria, Liver / Q000201:enzymology; D009711:Nucleotides / Q000494:pharmacology; D009840:Oligomycins / Q000494:pharmacology; D011092:Polyethylene Glycols; D051381:Rats; D013395:Sucrose / Q000494:pharmacology
chemical_list: D002414:Cations, Monovalent; D009711:Nucleotides; D009840:Oligomycins; D011092:Polyethylene Glycols; D013395:Sucrose; D000255:Adenosine Triphosphate; D003576:Electron Transport Complex IV; D006596:Hexosaminidases; D000251:Adenosine Triphosphatases; D008274:Magnesium; D002118:Calcium