[RNA-synthesizing complex of the Sendai virus in the nucleoli of infected Erlich ascitic carcinoma cells].
A virus-specific complex with sedimentation constant of 250S and buoyant density 1.35 g/cm3 in cesium chloride density gradient is formed within 20--24 hours after infection in nucleoli of Ehrlich ascitic carcinoma cells infected with Sendai virus. The complex contains a rapidly sedimenting RNA (70--90S) which is about 50% stable to RN-ase. The complex has RNA-polymerase activity in a cell-free system; the product of the RNA-polymerase reaction is a RNA with sedimentation constant 50S. It is suggested that the virus-specific structure with sedimentation constant 250 S found in nucleoli is the Sendai virus replicative complex.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000382:microbiology* / Q000648:ultrastructure; D002466:Cell Nucleolus / Q000201:enzymology / Q000382:microbiology*; D003593:Cytoplasm / Q000201:enzymology; D012321:DNA-Directed RNA Polymerases / Q000032:analysis; D010222:Parainfluenza Virus 1, Human / Q000378:metabolism*; D012367:RNA, Viral / Q000096:biosynthesis* / Q000302:isolation & purification; D013997:Time Factors; D014779:Virus Replication
D012367:RNA, Viral; D012321:DNA-Directed RNA Polymerases
RNK-sinteziruiushchiĭ kompleks virusa Sendaĭ v iadryshkakh zarazhennykh kletok astsitnoĭ kartsinomy Erlikha.