Synthesis and packaging of herpes simplex virus DNA in the course of virus passages at high multiplicity.
Herpes simplex virus type 1 (HSV-1), strain ANG, was passaged serially at high MOI or undiluted on RC-37 cells. The yields of infectious virus decreased and increased periodically with a maximum of about 3 log units variation in titer. The amount of newly synthesized HSV-1 DNA, however, did not vary by more than a factor of 2. On the other hand, the fraction of total HSV-1 DNA associated with virions was reduced at passage numbers coinciding with the minima in the yields of infectious progeny virus. Apparently, low yields of infectious virus mainly reflected a reduced efficiency of packaging of viral DNA into mature virus particles. One step in the process of virus maturation which appeared to be impaired was the assembly of nucleocapsids. HSV-1 ang was shown to be capable of generating different classes of defective particle in independent series of virus passages at high MOI.
D002213:Capsid / Q000096:biosynthesis; D004279:DNA, Viral / Q000096:biosynthesis*; D018139:Simplexvirus / Q000378:metabolism* / Q000472:pathogenicity; D013997:Time Factors; D014771:Virion / Q000378:metabolism; D014779:Virus Replication
D004279:DNA, Viral