title: Cellular basis of persistent lymphocytosis in cattle infected with bovine leukemia virus.
abstract: Peripheral blood lymphocytes from 14 cattle infected with the bovine leukemia virus (BLV) and 14 BLV-free cattle were examined by the membrane immunofluorescent antibody technique to detect surface immunoglobulin (S-Ig) and by the erythrocyte-antibody-complement (EAC) rosette test for the detection of complement receptors. Direct comparisons of the percentages of S-Ig-bearing cells and EAC rosette-forming cells in both infected and BLV-free animals showed no evidence for the presence of a substantial population bearing one surface marker but not the other. The data showed that cells with surface markers characteristic of B lymphocytes are responsible for most of the increase in peripheral blood lymphocytes which may accompany BLV infection. The release of infectious BLV and the spontaneous uptake of thymidine by short-term cultured peripheral blood lymphocytes from BLV-infected cattle were also studied. The results indicate that both of these activities are function of B lymphocytes.
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D002417:Cattle; D002478:Cells, Cultured; D003165:Complement System Proteins; D005455:Fluorescent Antibody Technique; D007104:Immune Adherence Reaction; D001909:Leukemia Virus, Bovine / Q000276:immunology / Q000302:isolation & purification; D008214:Lymphocytes / Q000276:immunology* / Q000378:metabolism; D008218:Lymphocytosis / Q000209:etiology / Q000276:immunology*; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D012190:Retroviridae / Q000302:isolation & purification; D013936:Thymidine / Q000378:metabolism
chemical_list: D000914:Antibodies, Viral; D011947:Receptors, Antigen, B-Cell; D003165:Complement System Proteins; D013936:Thymidine