Demonstration of host antigens in the myxovirus memebrane: lysis of virus by antibody and complement.
Complement-mediated lesions developed in myxovirus membranes in the presence of antibody to host antigens. Intact virions reacted with antibody to the keratosulphate-like host antigen present in the spike layer. Spike-deprived influenza virus reacted in the same way when an antiserum to the Forssman glycolipid was used. The holes in the membrane varied considerably in size, but were otherwise similar to those found in erythrocyte membranes. This immune electron microscopy method proved useful for the localization of host material in viral membranes.
D000914:Antibodies, Viral; D000956:Antigens, Viral / Q000032:analysis; D002462:Cell Membrane / Q000276:immunology; D003165:Complement System Proteins; D007158:Immunologic Techniques; D009980:Influenza A virus / Q000276:immunology; D008854:Microscopy, Electron; D009975:Orthomyxoviridae / Q000276:immunology*; D010222:Parainfluenza Virus 1, Human / Q000276:immunology
D000914:Antibodies, Viral; D000956:Antigens, Viral; D003165:Complement System Proteins