Cell-free translation of virion RNA from nondefective and transformation-defective Rous sarcoma viruses.
Nondefective and transformation-defective virion subunit RNAs from two strains of Rous sarcoma virus (RSV) were translated in cell-free systems derived from Krebs IIA ascites cells, wheat germ, and L-cells. In each case the predominant viral-specific product was a polypeptide of molecular weight 76,000 that is related to the internal viral group-specific antigens, as judged by immunoprecipitation with monospecific antisera and tryptic peptide fingerprinting. No difference could be detected between the translation products of 35S RNA from nondefective and transformation-defective RSV virions, nor of 35S RNA from different strains of RSV. The 76,000-molecular-weight polypeptide synthesized in response to 35S RNA in vitro was labeled with formyl-methionine from initiator tRNA. Models for viral protein synthesis are discussed in the light of these results, and arguments positioning the group-specific antigen gene at the 5' end of the 35S RNA are presented.
D000956:Antigens, Viral; D001358:Avian Sarcoma Viruses / Q000276:immunology / Q000378:metabolism*; D002471:Cell Transformation, Neoplastic; D002474:Cell-Free System; D003673:Defective Viruses / Q000378:metabolism*; D008970:Molecular Weight; D010452:Peptide Biosynthesis; D010455:Peptides / Q000276:immunology; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000378:metabolism*; D014764:Viral Proteins / Q000096:biosynthesis* / Q000276:immunology
D000956:Antigens, Viral; D010455:Peptides; D012367:RNA, Viral; D014764:Viral Proteins