Simian virus 40-Chinese hamster kidney cell interaction. IV. Enhanced virus replication in infected cells upon treatment with mitomycin C.
Chinese hamster kidney cells are semi-permissive to simian virus 40 (SV40). Exposure to mitomycin C (MC) of Chinese hamster kidney cells infected with SV40 DNA enhanced the yield of infectious virus 10- to 100-fold. This stimulation occurred whether the treatment was performed before or after infection. A simultaneous increase in the number of V antigen-synthesizing cells and virus-producing cells, as well as the virus burst size, was observed upon MC pretreatment, whereas the proportion of T antigen-synthesizing cells remained unchanged. MC pretreatment clearly stimulated virus DNA replication in SV40 virus-infected cells. Cells treated with MC exhibited an unbalanced growth pattern, with continuing protein synthesis in the absence of cell division and a markedly reduced ability to replicate the cellular DNA. These results suggest that MC enhances the permissiveness of Chinese hamster kidney cells by inducing the synthesis of a specific cellular factor(s) required for SV40 reproduction in Chinese hamster kidney cells.
D000818:Animals; D000956:Antigens, Viral; D002110:Caffeine / Q000494:pharmacology; D002455:Cell Division; D002460:Cell Line; D006224:Cricetinae; D004247:DNA / Q000096:biosynthesis; D004261:DNA Replication / Q000187:drug effects; D004279:DNA, Viral / Q000096:biosynthesis; D008937:Mitomycins / Q000494:pharmacology*; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis; D013539:Simian virus 40 / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D014466:Ultraviolet Rays; D014779:Virus Replication / Q000187:drug effects* / Q000528:radiation effects
D000956:Antigens, Viral; D004279:DNA, Viral; D008937:Mitomycins; D002110:Caffeine; D012313:RNA; D004247:DNA