The existence of triphosphorylated 5'-ends in virus-specific RNA isolated from SV-40 transformed cells.
The question about the nature of promoters in the transcriptional units containing SV-40 sequences in transformed cells was analyzed. It was found that the pulse-labeled RNA hybridizing to SV-40 DNA contains small but significant amounts of triphosphorylated 5'-ends detected as pppGp in alkaline hydrolyzates of this RNA. In another series of experiments the fragments of RNA containing triphosphorylated 5'-ends about 100 nucleotides in length have been isolated by hydroxyapatite chromatography. Some of them form hybrids with SV-40 DNA. The conclusion is drawn that at least some of SV-40 promoters are used for transcription initiation in SV-40 transformed cells.
D000469:Alkaline Phosphatase; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D002471:Cell Transformation, Neoplastic; D004279:DNA, Viral; D004926:Escherichia coli / Q000201:enzymology; D009693:Nucleic Acid Hybridization; D009843:Oligoribonucleotides / Q000032:analysis; D012367:RNA, Viral / Q000096:biosynthesis; D013539:Simian virus 40 / Q000378:metabolism*
D004279:DNA, Viral; D009843:Oligoribonucleotides; D012367:RNA, Viral; D000469:Alkaline Phosphatase