Infection of resistant avian cells by subgroup B Rous sarcoma virus.
Chicken fibroblasts derived from the H & N flock, which have been characterized as resistant to subgroup B avian oncornaviruses in focus assays, can be infected in suspension shortly after trypsinization by subgroup B sarcoma and leukosis viruses. Once cells are plated, resistance to infection reappears rapidly. C/BE cell suspensions obtained by treatment with EDTA instead of trypsin are not as sensitive to infection. Late interference established by preinfection with subgroup B leukosis viruses is not overcome by trypsinization. In addition to C/BE H & N chicken cells, C/ABE RPRL line 7 cells can also be infected by subgroup B viruses shortly after trypsinization; however, none of the cell types can be made sensitive to subgroup E infection. These results are discussed in relation to current information on the genetic control of resistance to avian oncornaviruses.
D000818:Animals; D001354:Avian Leukosis Virus / Q000254:growth & development; D001358:Avian Sarcoma Viruses / Q000145:classification / Q000254:growth & development*; D002460:Cell Line; D002642:Chick Embryo; D004492:Edetic Acid / Q000494:pharmacology; D011784:Quail; D014357:Trypsin / Q000494:pharmacology; D014762:Viral Interference; D014779:Virus Replication
D004492:Edetic Acid; D014357:Trypsin