Hydroxylation of aromatic compounds by reduced nicotinamide-adenine dinucleotide and phenazine methosulphate requires hydrogen peroxide and hydroxyl radicals, but not superoxide.
1. A mixture of NADH and phenazine methosulphate hydroxylates aromatic compounds at acidic pH values. 2. Hydroxylation is inhibited by catalase and by scavengers of the hydroxyl radical (-OH) but not by superoxide dismutase. 3. It is concluded that neither O2 leads to nor HO2- is sufficiently reactive to hydroxylate aromatic rings.
D006861:Hydrogen Peroxide; D062385:Hydroxybenzoates; D006900:Hydroxylation; D008773:Methylphenazonium Methosulfate; D009243:NAD; D010619:Phenazines; D013481:Superoxides
D062385:Hydroxybenzoates; D010619:Phenazines; D009243:NAD; D013481:Superoxides; D008773:Methylphenazonium Methosulfate; D006861:Hydrogen Peroxide