Simian virus 40-Chinese hamster kidney cell interaction. III. Characteristics of chemical induction in a clone of virogenic transformed cells.
A number of chemical and physical agents were screened to determine their effectiveness in inducing simian virus (SV40) production in a virogenic clone of SV40-transformed Chinese hamster cells. Mitomycin C (MC) was the most effective inducing agent, and MC induction was further characterized. It was found that levels of infectious SV40 DNA were increased above control levels as early as 6 h after addition of MC to the culture medium and reached maximum levels by 48 h. Virus capsid (V) antigen and virions followed with a lag of about 24 h. V antigen production was sensitive to hydroxyurea, suggesting a dependence on virus DNA synthesis. The proportion of virus-producing cells (infectious centres) and the virus burst per cell were both stimulated by MC. Studies of 3H-thmidine incorporation demonstrated that the rate of SV40 DNA synthesis was maximal at 48 h post-induction, at which time cellular DNA synthesis was almost abolished. Caffeine, at doses not toxic to non-induced cells, strongly inhibited SV40production in both non-induced and induced cells, suggesting some role for DNA repair mechanisms.
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002110:Caffeine / Q000494:pharmacology; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002999:Clone Cells; D006224:Cricetinae; D003513:Cycloheximide / Q000494:pharmacology; D004273:DNA, Neoplasm / Q000096:biosynthesis; D004279:DNA, Viral / Q000096:biosynthesis; D006918:Hydroxyurea / Q000494:pharmacology; D007065:Idoxuridine / Q000494:pharmacology; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D008937:Mitomycins / Q000494:pharmacology*; D013539:Simian virus 40 / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D014779:Virus Replication
D004273:DNA, Neoplasm; D004279:DNA, Viral; D008937:Mitomycins; D008769:Methylnitronitrosoguanidine; D002110:Caffeine; D003994:Bucladesine; D003513:Cycloheximide; D007065:Idoxuridine; D006918:Hydroxyurea