title: Ultrastructural study of avian synovium infected with an arthrotropic reovirus.
abstract: Three-week-old chicks were inoculated via the foot pad with the avian reovirus (strain WVU 2937) that has been proved to be the causative agent in avian viral arthritis. The initial stages of the avian reovirus infection in the synovium of the hock joint were studied by transmission electron microscopy to shed light on the early virus-host relationship of an arthrotropic virus and synovial tissue. At 48 hours postinoculation (PI), coated, partially coated, and uncoated virus particles were seen in a dense lysosome-like inclusion in the cytoplasm of subsynovial fibroblasts. Virus replication was demonstrated in fibroblasts of the subsynovium 4 days PI. Infiltration of leukocytes into the area of the virus replication was noted at the time of virus replication (4 days PI). By the second week of the infection (11 days PI), the subsynovium was heavily infiltrated with lymphocytes and plasma cells, and proliferative changes had occurred in the synovial lining cells.
mesh_terms: D000818:Animals; D001170:Arthritis, Infectious / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D002645:Chickens / Q000382:microbiology*; D003593:Cytoplasm / Q000382:microbiology / Q000648:ultrastructure; D004195:Disease Models, Animal; D005347:Fibroblasts / Q000382:microbiology / Q000648:ultrastructure; D006614:Hindlimb / Q000648:ultrastructure; D007181:Inclusion Bodies, Viral; D007962:Leukocytes / Q000382:microbiology / Q000648:ultrastructure; D008214:Lymphocytes / Q000382:microbiology / Q000648:ultrastructure; D010950:Plasma Cells / Q000382:microbiology / Q000648:ultrastructure; D011201:Poultry Diseases / Q000473:pathology*; D012087:Reoviridae / Q000472:pathogenicity; D012088:Reoviridae Infections / Q000473:pathology / Q000662:veterinary*; D013583:Synovial Membrane / Q000382:microbiology / Q000648:ultrastructure*; D013585:Synovitis / Q000473:pathology; D014779:Virus Replication