Lipid-protein interaction in the phosphatidylcholine exchange protein.
Incorporation of 2-acyl spin-labeled lecithin into the phosphatidylcholine protein from bovine liver results in an immobilization of the spin-label at the methyl and the carboxyl terminal end of the acyl chain. The nitroxide group on the protein-bound lecithin molecule is not accessible to ascorbate. This suggests that lecithin is buried in a pocket on the protein, which effectively shields the acyl chains from the medium.
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D001665:Binding Sites / Q000187:drug effects; D002352:Carrier Proteins / Q000378:metabolism; D002417:Cattle; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004578:Electron Spin Resonance Spectroscopy; D008099:Liver / Q000378:metabolism; D010713:Phosphatidylcholines / Q000378:metabolism; D011487:Protein Conformation; D013113:Spin Labels
D002352:Carrier Proteins; D010713:Phosphatidylcholines; D013113:Spin Labels; D001205:Ascorbic Acid