[Preparative method of isolating phosphoinositide fractions from brain tissue].
A simple technique for preparative isolation of chromatographically homogenous fractions of mono-, di- and triphosphoinositides from ox brain tissue is described. Podyphosphoinositides fractions were obtained after chromatography of lipid extract on DEAE cellulose, phosphomonoinositides fraction--after chromatography of polyphosphoinositide-free material on aluminium hydroxide column. Bivalent metal ions were eliminated from lipid extract using chromatography on Dowex-50 H+. Ammonium acetate was removed after precipitation of lipids in water: methanol (1:1) in the presence of 4 M this salt. The average yield of mono-, di- and triphosphates was 40, 22 and 58 mg respectively per 1 kg of brain tissue as callulated for lipid phosphorus.
D000537:Aluminum Oxide; D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002845:Chromatography / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D010716:Phosphatidylinositols / Q000302:isolation & purification*
D010716:Phosphatidylinositols; D000537:Aluminum Oxide
Preparativnyĭ metod polucheniia fraktsiĭ fosfoinozitidov iz tkani golovnogo mozga.