Effect of exogenous nucleotides on the candicidin fermentation.
Addition of cyclic-AMP (c-AMP) to Streptomyces griseus fermentations inhibited candicidin formation. In a phosphate-free resting cell system, c-AMP inhibited net candicidin formation and incorporation of labeled propionate and p-aminobenzoic acid into the antibiotic but did not inhibit protein synthesis. All nucleotides tested, regardless of the position of the phosphate ester, were effective inhibitors; nucleosides and free bases were not. Inhibition occurred whether the nucleotide was added early or late. The results indicate that inhibition of antibiotic formation by exogenous nucleotides, including cyclic nucleotides, is similar to the effect produced by inorganic phosphate.
D000225:Adenine / Q000494:pharmacology; D000227:Adenine Nucleotides / Q000494:pharmacology*; D000244:Adenosine Diphosphate / Q000494:pharmacology; D000249:Adenosine Monophosphate / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000935:Antifungal Agents / Q000096:biosynthesis*; D002174:Candicidin / Q000096:biosynthesis*; D000242:Cyclic AMP / Q000494:pharmacology*; D006152:Cyclic GMP / Q000494:pharmacology; D005285:Fermentation; D006150:Guanine Nucleotides / Q000494:pharmacology*; D012263:Ribonucleosides / Q000494:pharmacology; D013305:Streptomyces griseus / Q000187:drug effects* / Q000378:metabolism; D014500:Uracil Nucleotides / Q000494:pharmacology*; D014542:Uridine Monophosphate / Q000494:pharmacology*
D000227:Adenine Nucleotides; D000935:Antifungal Agents; D006150:Guanine Nucleotides; D012263:Ribonucleosides; D014500:Uracil Nucleotides; D002174:Candicidin; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D000242:Cyclic AMP; D014542:Uridine Monophosphate; D006152:Cyclic GMP; D000225:Adenine