title: Nucleotide sequence of the restriction fragment Hind F-Eco RI2 of SV40 DNA.
abstract: The nucleotide sequence of the SV40 genome region between the Hind K fragment and the Eco RI cleavage site has been determined by a combination of three different approaches : analysis of RNA products obtained by transcription with Escherichia coli DNA dependent RNA polymerase, partial degradations with snake venom exonuclease and base-specific chemical degradation of 5'-terminal labeled restriction fragments. This nucleotide sequence shows only one open reading frame and allows the deduction of a small segment of the amino acid sequence of VP1, the major structural protein.
mesh_terms: D000469:Alkaline Phosphatase; D001483:Base Sequence; D055598:Chemical Phenomena; D002621:Chemistry; D004262:DNA Restriction Enzymes; D004279:DNA, Viral; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D003854:Deoxyribonucleotides / Q000032:analysis / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology; D005092:Exonucleases; D005838:Genotype; D011116:Polynucleotide 5'-Hydroxyl-Kinase; D012313:RNA / Q000096:biosynthesis; D012260:Ribonucleases; D013539:Simian virus 40; D012910:Snake Venoms; D014158:Transcription, Genetic
chemical_list: D004279:DNA, Viral; D003854:Deoxyribonucleotides; D012910:Snake Venoms; D012313:RNA; D011116:Polynucleotide 5'-Hydroxyl-Kinase; D012321:DNA-Directed RNA Polymerases; D005092:Exonucleases; D012260:Ribonucleases; D004262:DNA Restriction Enzymes; D000469:Alkaline Phosphatase