title: The structure of polymyxin S. (Studies on antibiotics from the genus Bacillus. XXI).
abstract: Amino acid analysis on the acid hydrolyzate of polymyxin S1 revealed its amino acid composition. Isolation of the constituent amino acids and measurement of their optical activities clarified their chiralities: Dab(5L), Thr(3L), Ser(1D) and Phe(1D). The constituent fatty acid was identified with anteisononanoic acid by gas chromatography and mass spectrometry. By the action of polymyxin acylase, deacyl polymyxin S was obtained. Successive EDMAN degradation reaction on deacyl polymyxin S revealed the amino acid sequence. Further evidence for the structure of polymyxin S1 was obtained by partial acid hydrolysis on tetra-(DNP)-polymyxin S1.
mesh_terms: D000595:Amino Acid Sequence; D000613:Aminobutyrates / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D010649:Phenylalanine / Q000032:analysis; D011113:Polymyxins / Q000032:analysis*; D012694:Serine / Q000032:analysis; D013912:Threonine / Q000032:analysis
chemical_list: D000613:Aminobutyrates; D011113:Polymyxins; D013912:Threonine; D012694:Serine; D010649:Phenylalanine