Phytohemagglutinin enhancement of dengue-2 virus replication in nonimmune rhesus monkey peripheral blood leukocytes.
Phytohemagglutinin treatment of peripheral blood leukocytes from dengue nonimmune monkeys enhanced dengue-2 virus replication. Enhancement was due primarily to an increase in the number of infected cells. Destruction of mononuclear phagocytes with silica did not significantly inhibit virus replication in phytohemagglutinin-treated cultures. Pokeweed mitogen, concanavalin A, and streptolysin O stimulated increased deoxyribonucleic acid synthesis in monkey leukocytes but did not enhance virus replication. None of the mitogens significantly affected virus replication in cultures of dengue-immune monkey peripheral blood leukocytes.
D000818:Animals; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D003716:Dengue Virus / Q000187:drug effects* / Q000254:growth & development; D000882:Haplorhini; D037102:Lectins / Q000494:pharmacology*; D007962:Leukocytes / Q000382:microbiology*; D008253:Macaca mulatta; D008934:Mitogens / Q000494:pharmacology; D012822:Silicon Dioxide / Q000494:pharmacology; D013301:Streptolysins / Q000494:pharmacology; D014779:Virus Replication / Q000187:drug effects*
D037102:Lectins; D008934:Mitogens; D013301:Streptolysins; D003208:Concanavalin A; D012822:Silicon Dioxide