Changes in the collagenolytic activity released by primary VX-2 carcinoma cultures as a function of tumor growth.
Serum-free media of minced tissue cultures of VX-2 rabbit carcinoma contained a specific collagenolytic activity capable of releasing soluble radioactive peptides from [14C]-labeled collagen fibrils. It was also capable of reducing the viscosity of acid-soluble collagen solutions by cleaving the tropocollagen (TC) molecules primarily at one site to TCA (75%) and TCB (25%) fragments. Three chromatographic fractions were separated by gel filtration: F1, (MW 85-110,000) present in larger amounts in early cultures of younger tumor tissue; F2, (MW-35-40,000) the major component with maximum production in the day 3 media of younger and advanced tumor tissues; F3, (MW 18-22,000) the minor component. Early cultures of younger tumor tissue contained a latent collagenase and were subject to trypsin activation suggesting the presence of inactive enzyme precursors or an enzyme-inhibitor complex.
D000818:Animals; D002455:Cell Division; D003094:Collagen / Q000378:metabolism; D003470:Culture Media; D046508:Culture Techniques; D004789:Enzyme Activation; D004792:Enzyme Precursors / Q000378:metabolism; D006868:Hydrolysis; D003012:Microbial Collagenase / Q000378:metabolism*; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental; D011817:Rabbits; D013997:Time Factors; D014357:Trypsin / Q000494:pharmacology
D003470:Culture Media; D004792:Enzyme Precursors; D003094:Collagen; D014357:Trypsin; D003012:Microbial Collagenase