[Effect of insulin on activity of carbohydrate metabolism enzymes in loach embryos in early development].
It is established that in embryos incubated until the early blastula stage in the solution of insulin with addition of cycloheximide or puromycin, there is neither a decrease in the hexokinase and glucose-61 phosphate dehydrogenase activities nor an increase in the phosphofructokinase activity, as it is shown under the influence of insulin only. Puromycin removes an inhibitory effect of insulin on the glucose-6-phosphatase activity, and actinomycin D removes this influence with respect to glucose-6-phosphate dehydrogenase and glucose-6-phosphatase activities. The addition of antibiotics removes inhibition of the hexokinase, glucose-6-phosphate dehydrogenase and glucose-6-phosphatase activities by the hormone in the unfertilized eggs as well. Actinomycin D alone inhibits the hexokinase and activates the phosphofructokinase activities in the embryos and eggs, puromycin decreases their hexokinase activity and cycloheximide has the same effect on the glucose-6-phosphatase activity in the embryos only.
D000818:Animals; D050260:Carbohydrate Metabolism; D003513:Cycloheximide / Q000494:pharmacology; D004625:Embryo, Nonmammalian; D005260:Female; D005399:Fishes / Q000378:metabolism*; D005952:Glucose-6-Phosphatase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D005954:Glucosephosphate Dehydrogenase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006593:Hexokinase / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology*; D010063:Ovum / Q000201:enzymology; D010732:Phosphofructokinase-1 / Q000378:metabolism; D010770:Phosphotransferases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D011691:Puromycin / Q000494:pharmacology
D007328:Insulin; D011691:Puromycin; D003513:Cycloheximide; D005954:Glucosephosphate Dehydrogenase; D010770:Phosphotransferases; D006593:Hexokinase; D010732:Phosphofructokinase-1; D005952:Glucose-6-Phosphatase
Vpliv insulinu na aktivnist' fermentiv obminu vuglevodiv u v'iuna na rann'omu rozvitku.