Enzymatic synthesis of oligonucleotides of defined sequence: synthesis of a segment of yeast iso-1-cytochrome c gene.
The deoxyribooligonucleotide, d(pT-T-A-G-C-A-G-A-A-C-C-G-G), constituting a segment of yeast iso-1-cytochrome c gene, has been synthesized by a combination of chemical and primarily enzymatic methods. The starting primer, d(pT-T-A-G1, was chemically synthesized by the phosphodiester method and was extended stepwise, by reactions catalyzed by polynucleotide phosphorylase.
D003574:Cytochrome c Group; D005796:Genes; D009838:Oligodeoxyribonucleotides / Q000032:analysis / Q000138:chemical synthesis*; D009841:Oligonucleotides / Q000138:chemical synthesis*; D011117:Polyribonucleotide Nucleotidyltransferase / Q000378:metabolism*; D012441:Saccharomyces cerevisiae
D003574:Cytochrome c Group; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D011117:Polyribonucleotide Nucleotidyltransferase