Alteration of the growth of cytomegalovirus and herpes simplex virus type 1 by epidermal growth factor, a contaminant of crude human chorionic gonadotropin preparations.
Pretreatment (12-48 h) of human fibroblasts with crude, human chorionic gonadotropin (HCG) was found to suppress cytomegalovirus infection and enhance productive herpes simplex type 1 (HSV) infection in vitro. Maximal effect on virus replication occurred at the time of maximal infectivity of control cultures (48 h and 6 days after viral innoculation for HSV and cytomegalovirus, respectively). The alteration in viral growth was not due to the HCG itself, but rather to epidermal growth factor, a contaminant of crude HCG. The effect of epidermal growth factor on viral infectivity was shown to be a cell-mediated event requiring protein synthesis.
D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D003587:Cytomegalovirus / Q000187:drug effects* / Q000254:growth & development / Q000472:pathogenicity; D004340:Drug Contamination; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D010455:Peptides / Q000494:pharmacology*; D018139:Simplexvirus / Q000187:drug effects* / Q000254:growth & development / Q000472:pathogenicity
D006063:Chorionic Gonadotropin; D010455:Peptides; D004815:Epidermal Growth Factor