[Separation of catalytic and regulatory subunits of pigeon breast muscle protein kinase].
The separation of catalytic cAMP-independent and regulatory cAMP-binding subunits of pigeon breast muscle protein kinase was performed after the treatment of the holoenzyme by cAMP. The molecular weight of the homogeneous catalytic subunit is determined as 30,000. When catalytic and regulatory subunits were mixed, the decrease of phosphotransferase activity was observed.
D000818:Animals; D002384:Catalysis; D010856:Columbidae / Q000378:metabolism*; D000242:Cyclic AMP; D046911:Macromolecular Substances; D008970:Molecular Weight; D009132:Muscles / Q000201:enzymology*; D011494:Protein Kinases / Q000302:isolation & purification* / Q000378:metabolism
D046911:Macromolecular Substances; D000242:Cyclic AMP; D011494:Protein Kinases
Razdelenie proteinkinazy iz grudnoĭ myshtsy golubia na kataliticheskuiu i reguliatornuiu sub'edinitsy.