Anion-exchange chromatography of erythrocytic and muscle adenylate kinase and its effect on the serum creatine kinase isoenzyme assays.
We determined the elution profile of erythrocytic and muscle adenylate kinases (EC 2.7.4.3) in the Roche chromatographic creatine kinase procedure and studied the interference these enzymes would cause in the isolation and assay of serum creatine kinase (EC 2.7.3.2) isoenzymes. Both adenylate kinases co-elute with the creatine kinase MM fraction and do not interfere with the isolation or assay of the MB fraction.
D000263:Adenylate Kinase / Q000097:blood / Q000302:isolation & purification*; D000818:Animals; D002852:Chromatography, Ion Exchange / Q000379:methods; D003402:Creatine Kinase / Q000097:blood*; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D007527:Isoenzymes / Q000097:blood*; D009132:Muscles / Q000201:enzymology*; D010770:Phosphotransferases / Q000302:isolation & purification*; D011817:Rabbits
D007527:Isoenzymes; D010770:Phosphotransferases; D003402:Creatine Kinase; D000263:Adenylate Kinase