Potentiation of the NGF-mediated nerve fiber outgrowth by ginsenoside Rb1 in organ cultures of chicken dorsal root ganglia.
The fiber outgrowth induced by ACh, dibutyryl cyclic AMP and dibutyryl cyclic GMP in explanted chick embryonic dorsal root ganglia differed distinctly from that be nerve growth factor (NGF) and submandibular gland extract of adult male mice. Ginsenoside Rb1 potentiated the effects of NGF and submandibular extract at concentrations of 3 and 30 muM, but did not potentiate the effects of ACh, dibutyryl cyclic AMP and dibutyryl cyclic GMP. NGF-antibody inhibited the effects of NGF, but not the effects of ACh, dibutyryl cyclic AMP and dibutyryl cyclic GMP, Concanavalin A and KCL did not promote fiber outgrowth.
D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D000906:Antibodies / Q000032:analysis; D003994:Bucladesine / Q000494:pharmacology; D002642:Chick Embryo; D003208:Concanavalin A / Q000494:pharmacology; D003995:Dibutyryl Cyclic GMP / Q000494:pharmacology; D004357:Drug Synergism; D005727:Ganglia, Spinal; D008297:Male; D051379:Mice; D009412:Nerve Fibers / Q000254:growth & development*; D009414:Nerve Growth Factors / Q000037:antagonists & inhibitors / Q000276:immunology / Q000494:pharmacology*; D009924:Organ Culture Techniques; D005894:Panax; D037121:Plant Lectins; D010946:Plants, Medicinal; D011188:Potassium / Q000494:pharmacology; D012503:Saponins / Q000494:pharmacology*; D013363:Submandibular Gland / Q000032:analysis
D000906:Antibodies; D009414:Nerve Growth Factors; D037121:Plant Lectins; D012503:Saponins; D003208:Concanavalin A; D003995:Dibutyryl Cyclic GMP; D003994:Bucladesine; D000109:Acetylcholine; D011188:Potassium