Glial cell characteristics in bulk-prepared cell fractions from human brain tumours.
The heterogeneity of brain tumours, especially in the glioblastoma group, makes biochemical characterization of pieces of the tumours hazardous even with extensive histological controls. This study employs a technique by which separate cell populations are subsequently isolated from the tumours by means of density gradient centrifugation. Cells isolated from glial brain tumours with low density sedimentation rates show the highest levels of glial cell characteristics, i.e. S-100 content and active uptake of the neurotransmitter GABA.
D001254:Astrocytoma / Q000378:metabolism / Q000648:ultrastructure*; D001932:Brain Neoplasms / Q000378:metabolism / Q000648:ultrastructure*; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D005909:Glioblastoma / Q000378:metabolism / Q000648:ultrastructure*; D006801:Humans; D008579:Meningioma / Q000378:metabolism / Q000648:ultrastructure*; D009457:Neuroglia / Q000378:metabolism / Q000648:ultrastructure*; D009418:S100 Proteins / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D009418:S100 Proteins; D005680:gamma-Aminobutyric Acid