Polyacrylamide gel electrophoretic analysis of herpes simplex virus type 1 immunoprecipitates obtained by quantitative immunoelectrophoresis in antibody-containing agarose gel.
Crossed immunoelectrophoresis was used to characterize herpes simplex virus type 1 (HSV-1) antigens produced by infected HEp-2 cells. We report on a method for analyzing the polypeptide content in individual antigen-antibody precipitates eluted from the second-dimensional agarose gel. Four glycoprotein antigens of HSV-1, Ag-8, Ag-11, Ag-6, and Ag-3, were isolated and analyzed for polypeptide content. The molecular weights of the polypeptides are presented.
D000956:Antigens, Viral / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000032:analysis / Q000276:immunology; D007122:Immunoelectrophoresis; D007123:Immunoelectrophoresis, Two-Dimensional; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis; D018139:Simplexvirus / Q000032:analysis / Q000276:immunology*; D014764:Viral Proteins / Q000032:analysis / Q000276:immunology
D000956:Antigens, Viral; D006023:Glycoproteins; D010455:Peptides; D014764:Viral Proteins