Biochemical reactions involved in pancreatic enzyme secretion. 4. Effects of cytochalasin B on functions of the exocrine pancreas.
These experiments were performed to evaluate the effects of cytochalasin B on pancreatic enzyme secretion and thus perhaps establish a role for microfilaments in the exocytosis process. The alkaloid at a concentration of 1 microgram/ml (2micron) inhibits amylase secretion induced by urecholine or cholecystokinin-pancreozymin (CCK-PZ) but does not modify that induced by dibutyryl cyclic AMP. The inhibitory effect of the drug is reversible after a 30-min washing out period. It does not affect O2 consumption, basal calcium efflux, or efflux caused by CCK-PZ. Amino acid accumulation in the tissue and their incorporation into proteins are not modified. It is suggested that cytochalasin B inhibits pancreatic enzyme secretion, probably through an effect on the microfilament system.
D000681:Amylases / Q000378:metabolism; D000818:Animals; D001626:Bethanechol Compounds / Q000494:pharmacology; D003994:Bucladesine / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002766:Cholecystokinin / Q000494:pharmacology; D003571:Cytochalasin B / Q000494:pharmacology*; D004347:Drug Interactions; D066298:In Vitro Techniques; D008297:Male; D010101:Oxygen Consumption / Q000187:drug effects; D010179:Pancreas / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D010649:Phenylalanine / Q000378:metabolism; D051381:Rats; D013997:Time Factors
D001626:Bethanechol Compounds; D003571:Cytochalasin B; D010649:Phenylalanine; D003994:Bucladesine; D002766:Cholecystokinin; D000681:Amylases; D002118:Calcium