Rescue of a transforming virus from a spontaneous nonproducing osteosarcoma in BALB/c mice.
Cells from spontaneous osteosarcoma V793 that originated in a 19-month-old female BALB/c mouse were cultured. They did not produce a C-type oncovirus as determined by extracellular reverse transcriptase assay and cytoplasmic immunofluorescence. After cocultivation with Balb/3T3 cells chronically infected with a murine leukemia virus (MuLV), a focus-forming principle that transformed 3T3 cells, secondary BALB/c mouse embryo and WAG/Rij rat embryo fibroblasts were rescued. The transformation could be inhibited by antiserum to MuLV.
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D003673:Defective Viruses / Q000302:isolation & purification; D017988:Gammaretrovirus / Q000302:isolation & purification*; D006378:Helper Viruses; D009052:Leukemia Virus, Murine; D051379:Mice; D012516:Osteosarcoma / Q000382:microbiology; D009053:Sarcoma Viruses, Murine / Q000302:isolation & purification*; D012513:Sarcoma, Experimental / Q000382:microbiology*