Cytomegalovirus proteins: II. Polypeptide composition of cytomegalovirus complement-fixing antigen.
Complement-fixing (CF) antigen, prepared from cytomegalovirus (CMV)-infected cells and solubilized by urea and Tween-20, was fractionated on agarose gel columns. Its molecular weight was 1.5--5 X 10(7) daltons, and it contained 5 major peptides with molecular weights from 60,000--90,000 daltons. The peptide with a molecular weight of 70,000 daltons which was present in the solubilized CF antigen was absent in a soluble fraction from infected cells without CF activity and may be, therefore, important in this respect. The antigens present in the CF antigen did not elicit neutralizing antibody.
D000956:Antigens, Viral / Q000032:analysis*; D003168:Complement Fixation Tests; D003587:Cytomegalovirus / Q000032:analysis* / Q000276:immunology; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis*; D012995:Solubility; D014764:Viral Proteins / Q000032:analysis* / Q000276:immunology
D000956:Antigens, Viral; D010455:Peptides; D014764:Viral Proteins