Inhibition of spermiation in the Syrian hamster using dibutyryl cyclic-AMP.
Young adult male Syrian hamsters were given intraperitoneal injections of 50 mg dibutyryl cyclic AMP twice daily for a period of three days. On the fourth day the animals were sacrificed and their testes were processed for light and electron microscopy. The results indicate that the mature spermatozoa were retained within the seminiferous epithelium after the stage in the seminiferous cycle in which spermiation normally occurs. The unreleased spermatozoa were ultrastructurally normal. Typical Sertoli-spermatid junctional specializations remained associated with the retained spermatozoa. These findings indicate that normally spermiation is initiated by the disappearance of the junctional specializations. In addition, the present results demonstrate that spermiation can be controlled.
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology*; D006224:Cricetinae; D008297:Male; D012670:Seminiferous Epithelium / Q000648:ultrastructure; D012708:Sertoli Cells / Q000187:drug effects* / Q000648:ultrastructure; D013087:Spermatids / Q000648:ultrastructure; D013091:Spermatogenesis / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects / Q000648:ultrastructure
D003994:Bucladesine