Dopamine-sensitive adenylate cyclase in the rat kidney particulate preparation.
Dopamine, apomorphine, isoproterenol and norepinephrine all increased the concentration of adenosine 3',5'-monophosphate in the rat kidney particulate preparation, which was composed of tubules, glomeruli and blood vessels. The concentrations of dopamine, apomorphine, isoproterenol and norepinephrine causing a half-maximal increase were 50, 83, 0.1 and 10 muM, respectively. The alpha-blocker, phentolamine, at a concentration as high as 1 mM, did not significantly reduce the effect of these drugs on the adenosine 3',5'-monophosphate concentration. The beta-blocker, propranolol (50 muM), blocked the effect of isoproterenol and norepinephrine, but not that of dopamine. The effect of dopamine was selectively blocked by spiroperidol (50 muM), a dopamine receptor antagonist, whereas the effects of isoproterenol and norepinephrine was not blocked by spiroperidol. These results suggest that in the rat kidney particulate preparation there is a specific dopamine receptor which can lead to the increase in the concentration of adenosine 3',5'-monophosphate.
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002395:Catecholamines / Q000494:pharmacology; D000242:Cyclic AMP / Q000096:biosynthesis; D004298:Dopamine / Q000494:pharmacology* / Q000502:physiology; D018492:Dopamine Antagonists; D004347:Drug Interactions; D066298:In Vitro Techniques; D007668:Kidney / Q000201:enzymology*; D008297:Male; D010646:Phentolamine / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology; D051381:Rats; D013134:Spiperone / Q000494:pharmacology; D013268:Stimulation, Chemical; D013806:Theophylline / Q000494:pharmacology
D002395:Catecholamines; D018492:Dopamine Antagonists; D013134:Spiperone; D011433:Propranolol; D013806:Theophylline; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D004298:Dopamine; D010646:Phentolamine