title: [Effect of deep freezing on isolated rat liver lysosomes].
abstract: The level of the non--sedimentating activity of acid hydrolases (deoxyribonuclease, phosphatase, cathepsins) and electron microscopy of lysosomes has been studied after freezing to --30 degrees, --70 degrees, --140 degrees and --196 degrees. It has been found that enzyme solubilistion and lysosome ultrastructure distortion are mostly marked in the temperature range between 0 degrees and --30 degrees C. Additional membrane damage is observed in the temperature range from --140 degrees to --196 degrees C. It is suggested that not only physico-chemical changes during phase transitions of free water in the freezing medium but also recrystallization processes and the freezing-out of water structurally bound with membranes may contribute to mechanism of lysosome cryoinjury.
mesh_terms: D000818:Animals; D002403:Cathepsins / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism; D005615:Freezing; D066298:In Vitro Techniques; D008099:Liver / Q000201:enzymology / Q000648:ultrastructure*; D008247:Lysosomes / Q000201:enzymology*; D008854:Microscopy, Electron; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D051381:Rats; D012260:Ribonucleases / Q000378:metabolism; D013696:Temperature
chemical_list: D003851:Deoxyribonucleases; D012260:Ribonucleases; D010744:Phosphoric Monoester Hydrolases; D002403:Cathepsins
vernacular_title: Vliianie glubokogo zamorazhivaniia na izolirovannye lizosomy pecheni krys.