Hormonal control of fat cells adenylate cyclase.
Fat cells were preincubated for 2 h in the presence and absence of growth hormone (GH) and Dexamethasone (Dex) before the addition of increasing concentrations of either epinephrine, theophylline or glucagon and final incubation of the cells for an additional 5 minutes. GH and Dex increased by 85%, 28% and 72%, respectively, the cAMP levels reached in the sole presence of 10(-5)M epinephrine, 10(-2)M theophylline or 5 X 10(-5)M glucagon. An adenylate cyclase particulate preparation shows that epinephrine decreases Km from 2mM to 0.6 MM and increases Vmax and the strength of interaction value (n) from 0.91 to 1.75.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000262:Adenylyl Cyclases / Q000378:metabolism*; D000273:Adipose Tissue / Q000166:cytology / Q000201:enzymology* / Q000378:metabolism; D000818:Animals; D000242:Cyclic AMP / Q000096:biosynthesis; D003907:Dexamethasone / Q000494:pharmacology; D004357:Drug Synergism; D004789:Enzyme Activation; D004822:Epididymis; D004837:Epinephrine / Q000494:pharmacology; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D005934:Glucagon / Q000494:pharmacology; D013006:Growth Hormone / Q000494:pharmacology; D006728:Hormones / Q000502:physiology*; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D051381:Rats; D013806:Theophylline / Q000494:pharmacology
D005230:Fatty Acids, Nonesterified; D006728:Hormones; D003907:Dexamethasone; D000255:Adenosine Triphosphate; D013006:Growth Hormone; D005934:Glucagon; D013806:Theophylline; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D004837:Epinephrine