Characterization of an adenylate cyclase system sensitive to histamine H2-receptor excitation in cells from dog gastric mucosa.
A method of preparing viable cells from dog gastric mucosa is described. Cyclic AMP in these cells is elevated by histamine and 4-methyl histamine but 2-methyl histamine is only a weak agonist. The effects on cyclic AMP levels are inhibited competitively by metiamide and burimamide which give apparent KBvalues of 3.5x10-7 M and 2.3x10-6 M, respectively. These values are similar to those reported for other histamine H2-receptor systems. The H1-receptor antagonists, mepyramine and chlorpheniramine, have no inhibitory effect on the histamine induced elevation of cyclic AMP: promethazine inhibits the system but not by a competitive mechanism. It is concluded that the histamine stimulated adenylate cyclase system is probably located in the parietal cell component.
D000067956:Adenylyl Cyclase Inhibitors; D000818:Animals; D002049:Burimamide / Q000494:pharmacology; D002477:Cells; D002744:Chlorpheniramine / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism; D004285:Dogs; D005753:Gastric Mucosa / Q000201:enzymology*; D006632:Histamine / Q000494:pharmacology; D006633:Histamine Antagonists; D006635:Histamine H2 Antagonists; D066298:In Vitro Techniques; D007700:Kinetics; D008761:Methylhistamines / Q000494:pharmacology; D008785:Metiamide / Q000494:pharmacology; D011398:Promethazine / Q000494:pharmacology; D011738:Pyrilamine / Q000494:pharmacology
D000067956:Adenylyl Cyclase Inhibitors; D006633:Histamine Antagonists; D006635:Histamine H2 Antagonists; D008761:Methylhistamines; D008785:Metiamide; D002744:Chlorpheniramine; D006632:Histamine; D000242:Cyclic AMP; D011398:Promethazine; D011738:Pyrilamine; D002049:Burimamide