hCG suppression of LH receptors and responsiveness of testicular tissue to hCG.
A single injection of 75 IU of human chorionic gonadotropin (hCG) into adult male rats caused a dramatic reduction in the concentration of membrane receptors for luteinizing hormone (LH) in the testis. The mean receptor level reached a nadir which was 5--10% of that in the control testes, 3 days after the injection, after which it gradually returned toward normal. This cannot be due to increased competition caused by the injected hCG since no decrease was observed at a time when the circulating levels of hCG were at a maximum (2--24 h after injection). Furthermore, at a time when receptor levels had been maximally reduced, circulating hCG was at or below the level of detection. Reduction in the number of LH binding sites in the testis was associated with a decreased responsiveness of the testicular tissue to hCG as measured by hCG-stimulated testosterone production in vitro. This inhibitory effect of large quantities of LH on its own receptor is suggested as a possible explantation for the previously observed low concentrations of LH receptor in the testis of the testicular feminized male (tfm) rat. This syndrome is characterized by high endogenous levels of plasma LH (Sherins et al., 1971).
D000818:Animals; D001665:Binding Sites; D002462:Cell Membrane / Q000378:metabolism; D006063:Chorionic Gonadotropin / Q000097:blood / Q000494:pharmacology*; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D007986:Luteinizing Hormone / Q000378:metabolism*; D008297:Male; D011485:Protein Binding; D051381:Rats; D011956:Receptors, Cell Surface / Q000378:metabolism*; D013737:Testis / Q000378:metabolism*; D013739:Testosterone / Q000097:blood
D006063:Chorionic Gonadotropin; D011956:Receptors, Cell Surface; D013739:Testosterone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone