Induction of proliferation in dense non-starved 3T3 cells by Ca++ ionophore A23187.
The proliferogenic effect of the Ca++ ionophore A23187 was tested in dense non-starved 3T3 cells. Whereas continuous exposure during 48 h of cells to the ionophore at concentrations is larger than or equal to 0.4 muM cytotoxic, a short exposure for 30 s up to 4 min at 0.2 muM was proliferogenic. It was also found that such short exposures to the ionophore caused a transient increase in the intracellular level of cyclic GMP and a roughly simultaneously appearing decrease in the intracellular level of cyclic AMP.
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000001:Calcimycin / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis; D005347:Fibroblasts; D008938:Mitosis / Q000187:drug effects*
D000900:Anti-Bacterial Agents; D000001:Calcimycin; D004247:DNA; D000242:Cyclic AMP; D006152:Cyclic GMP; D002118:Calcium