A simple method for concentration of enteroviruses and rotaviruses from cell culture harvests using membrane filters.
Organic compounds in cell culture harvests known as membrane-coating components (MCC) prevent virus adsorption to membrane filters. Blending cell culture harvests with fluorocarbon removed the MCC and permitted adsorption of virus in acidified harvests to epoxy-fiberglass filters. Subsequent elution with high pH buffer resulted in recovery of greater than 90% of the virus with concentrations of up to 100-fold.
D004770:Enterovirus / Q000302:isolation & purification*; D029822:Enterovirus B, Human / Q000302:isolation & purification*; D005374:Filtration / Q000295:instrumentation; D005466:Fluorocarbons; D017955:Poliovirus / Q000302:isolation & purification*; D012328:RNA Viruses / Q000302:isolation & purification*; D012401:Rotavirus / Q000302:isolation & purification*; D014776:Virus Cultivation / Q000379:methods*
D005466:Fluorocarbons