title: Primary action of steroid hormone at the surface of amphibian oocyte in the induction of germinal vesicle breakdown.
abstract: An insoluble steroid derivative was prepared by the coupling of desoxycorticosterone with aminoethylated agarose beads. When the naked full-grown Xenopus oocytes were incubated in contact with the steroid-bound agarose beads, the dissociation of oocyte nuclei or the initial step in the maturation of amphibian oocyte was induced in 30-100% of total oocytes. The induction did not occur in the oocytes covered with follicle cells and when the naked oocytes were placed apart from the steroid-bound agarose beads in the medium. The above findings confirmed that the oocyte surface was a primary site for this particular steroid action, eliminating the possibility of participation of free steroid artifactually released from the agarose. The 105,000g supernatant fraction of oocytes showed no sign of the presence of steroid receptor. This was not inconsistent with the assumption mentioned above.
mesh_terms: D000818:Animals; D003900:Desoxycorticosterone / Q000494:pharmacology*; D005260:Female; D009865:Oocytes / Q000187:drug effects / Q000502:physiology*; D010063:Ovum / Q000502:physiology*; D011374:Progesterone / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000302:isolation & purification / Q000378:metabolism; D012685:Sepharose; D014981:Xenopus
chemical_list: D011956:Receptors, Cell Surface; D003900:Desoxycorticosterone; D011374:Progesterone; D012685:Sepharose