Triacylglycerol biosynthesis in the adipose tissue of the obese-hyperglycaemic mouse.
Obesity in obese-hyperglycaemic mouse is associated with an increase in number and size of adipocytes. Adipocytes from the obese mouse showed increased incorporation of [14C]acetate and[14C]glucose into triacylglycerol. This increased capacity of triacylglycerol formation was correlated with increased activities of various triacylglycerol-forming enzymes measured in the microsomal fraction of adipose tissue from obese mice. Microsomal fractions from lean and obese mice contained sn-glycerol 3-phosphate acyltransferase, phosphatidate phosphohydrolase and diacylglycerol acyltransferase. Phosphatidate phosphohydrolase was also detected in the soluble fraction. In the presence of Mg2+, the phosphatidate phsophohydrolase from the soluble and the microsomal fractions was active towards membrane-bound phosphatidate. Among the three enzymes studied here, the increase in Mg2+-dependent phosphatidate phosphohydrolase was most prominent in adipose tissue of obese mice.
D000085:Acetates / Q000378:metabolism; D000217:Acyltransferases / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000273:Adipose Tissue / Q000201:enzymology / Q000378:metabolism*; D000818:Animals; D002458:Cell Fractionation; D003065:Coenzyme A / Q000494:pharmacology; D004822:Epididymis / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D005992:Glycerol-3-Phosphate O-Acyltransferase / Q000378:metabolism; D008274:Magnesium / Q000494:pharmacology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D008861:Microsomes / Q000201:enzymology / Q000378:metabolism; D010168:Palmitates / Q000494:pharmacology; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D014280:Triglycerides / Q000096:biosynthesis*
D000085:Acetates; D010168:Palmitates; D014280:Triglycerides; D000255:Adenosine Triphosphate; D000217:Acyltransferases; D005992:Glycerol-3-Phosphate O-Acyltransferase; D010744:Phosphoric Monoester Hydrolases; D008274:Magnesium; D005947:Glucose; D003065:Coenzyme A