A general method for purification of deoxycytidine kinase.
Deoxycytidine kinase from a variety of animal tissues binds to Cibacron Blue 3G-A coupled to Sepharose CL-6B. The enzyme can be selectively eluted with ATP to yield single-step purifications of 17-89-fold. The affinity adsorbent is re-usable.
D000818:Animals; D002846:Chromatography, Affinity; D003842:Deoxycytidine Kinase / Q000302:isolation & purification*; D010770:Phosphotransferases / Q000302:isolation & purification*
D010770:Phosphotransferases; D003842:Deoxycytidine Kinase