title: The isolation and preliminary genetic classification of temperature-sensitive mutants of bluetongue virus.
abstract: Temperature-sensitive mutants of bluetongue virus were isolated and classified in 6 genetic recombination groups. The frequency of recombination varied both within and between groups. The 4 mutagens used viz. nitrous acid, N-methyl-N-nitroso-N-nitroguanidine, proflavine and 5-fluoro-uracil were found to differ in their efficacy. The period of incubation required for maximum recombination was 48 h at 28 degrees C.
mesh_terms: D001820:Bluetongue virus / Q000145:classification / Q000302:isolation & purification*; D007700:Kinetics; D009154:Mutation; D011995:Recombination, Genetic; D012087:Reoviridae / Q000302:isolation & purification*; D013696:Temperature