Complete loss of heparin-releasable triacylglycerol lipase activity after collagenase treatment of the rat liver.
Lipolytic activity at pH 8.5-9.5 was lowered by approx. 80% in homogenates from livers perfused with collagenase or heparin. No heparin-releasable lipase activity was detected in hepatocytes isolated by collagenase treatment. It is concluded that crude collagenase completely inactivates the plasma-membrane-bound heparin-releasable lipase.
D000818:Animals; D006493:Heparin / Q000494:pharmacology; D066298:In Vitro Techniques; D008049:Lipase / Q000037:antagonists & inhibitors*; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D003012:Microbial Collagenase / Q000494:pharmacology*; D051381:Rats
D006493:Heparin; D008049:Lipase; D003012:Microbial Collagenase