Stimulation of amniotic fluid cells by fibroblast growth factor.
Amniotic fluid cells were grown in modified Dulbecco's medium with and without the following additives in different combinations: fibroblast growth factor (FGF), dexamethasone (DME), insulin and cyclic GMP under controlled conditions. Experiments were also conducted with varying amounts of foetal calf serum and FGF to establish optimum levels. Comparative growth, determined by measuring 3H-thymidine uptake, showed that the combination of FGF, DME and insulin in medium with 20% foetal calf serum produced the highest growth rate. These experiments indicate that the culture time of amniotic fluid cells for chromosome studies could be reduced by 25%. FGF did not have any deleterious effect on the chromosomes.
D000653:Amniotic Fluid / Q000166:cytology*; D002455:Cell Division / Q000187:drug effects*; D002478:Cells, Cultured; D003470:Culture Media; D006152:Cyclic GMP / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology; D005260:Female; D005347:Fibroblasts; D006133:Growth Substances / Q000494:pharmacology*; D006801:Humans; D007328:Insulin / Q000494:pharmacology; D011247:Pregnancy; D012709:Serum Albumin / Q000494:pharmacology; D013268:Stimulation, Chemical; D013997:Time Factors
D003470:Culture Media; D006133:Growth Substances; D007328:Insulin; D012709:Serum Albumin; D003907:Dexamethasone; D006152:Cyclic GMP