Isolation and preliminary characterization of temperature-sensitive mutants of encephalomyocarditis virus.
Thirty temperature-sensitive mutants of encephalomyocarditis virus have been isolated and partially characterized. Fifteen of these mutants are phenotypically RNA+ thirteen are RNA-, and two are RNA +/-. Six RNA + mutants, one RNA- mutants, and one RNA +/- mutant have virions which are more thermosensitive at 56 degree C than the wild-type virions. Hela cells infected at the nonpermissive temperature with any of the RNA+ mutants produced neither infective nor noninfective viral particles. The cleavage of the precursor polypeptides in cells infected with 11 of the RNA+ mutants was defective at the nonpermissive temperature. This defect in cleavage occurred only in those precursor polypeptides leading to capsid proteins.
D002213:Capsid / Q000378:metabolism*; D004686:Encephalomyocarditis virus / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D005814:Genes, Viral; D009154:Mutation; D011498:Protein Precursors / Q000378:metabolism*; D012367:RNA, Viral / Q000096:biosynthesis; D013696:Temperature; D014764:Viral Proteins / Q000378:metabolism*
D011498:Protein Precursors; D012367:RNA, Viral; D014764:Viral Proteins