Scanning electron microscopic studies on the cytolytic effect of phallolysin on isolated rat hepatocytes and AS-30 D hepatoma cells.
Rat hepatocytes and AS-30 D hepatoma cells were treated in vitro with phallolysin, the toxic haemolysin from the death cap fungus Amanita phalloides. Scanning electron microscopy revealed dose-dependent formation of bulky protrusions on the cell surface, which later on burst, expelling intracellular material into the surrounding medium, while the cells became stainable with trypan blue. After 1 haemolytic unit (HU)/ml the protrusions burst within 10 min; after 3 HU/ml all cells were destroyed within 3-5 min. Susceptibility of hepatoma cells did not differ from that of hepatocytes.
D000546:Amanitins / Q000633:toxicity*; D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000648:ultrastructure*; D005260:Female; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects* / Q000648:ultrastructure; D008113:Liver Neoplasms / Q000648:ultrastructure*; D008855:Microscopy, Electron, Scanning; D009374:Neoplasms, Experimental / Q000648:ultrastructure; D051381:Rats
D000546:Amanitins; C008824:phallolysine