Occurrence of steroid glucuronyltransferases in a hepatoma.
Occurrence of estrone, estradiol, and testosterone glucuronyltranferase activities was tested in a well-differentiated hepatoma, Reuber H35. Transferase activities for estrone and estradiol were found in the hepatoma. The Michaelis-Menten kinetics of these two microsomal glucuronyltransferase activities were similar in hepatoma and in liver preparations, except for a somewhat higher apparent Km for estradiol in the hepatoma preparations. Under the same experimental conditions, only trace amounts of testosterone glucuronyltransferase activity could be detected in the hepatoma preparations. By contrast, in liver microsomal preparations, testosterone glucuronyltransferase activity was the highest among the steroid glucuronyltransferase activities tested.
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000201:enzymology*; D004958:Estradiol / Q000378:metabolism; D004970:Estrone / Q000378:metabolism; D005965:Glucuronates / Q000378:metabolism; D014453:Glucuronosyltransferase / Q000378:metabolism; D006602:Hexosyltransferases / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D008113:Liver Neoplasms / Q000201:enzymology*; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology; D009374:Neoplasms, Experimental / Q000201:enzymology; D009596:Nitrophenols / Q000378:metabolism; D051381:Rats; D011912:Rats, Inbred ACI; D013739:Testosterone / Q000378:metabolism; D014535:Uridine Diphosphate Glucuronic Acid / Q000008:administration & dosage / Q000378:metabolism
D005965:Glucuronates; D009596:Nitrophenols; D014535:Uridine Diphosphate Glucuronic Acid; D004970:Estrone; D013739:Testosterone; D004958:Estradiol; D006602:Hexosyltransferases; D014453:Glucuronosyltransferase