Synthesis and properties of a photoaffinity probe for the glucagon receptor in hepatocyte plasma membranes.
The reaction of glucagon with 4-fluoro-3-nitrophenylazide has been shown to afford the photosensitive derivative, Nepsilon-4-azido-2-nitrophenyl-glucagon. The structure and properties of this derivative were established by amino acid analysis, absorption and fluorescence spectroscopy, deamination, Edman degradation and photolysis. This photoaffinity derivative of glucagon has been used to label specifically glucagon binding sites on hepatocyte plasma membranes.
D000596:Amino Acids / Q000032:analysis; D001386:Azides; D002462:Cell Membrane; D005464:Fluorobenzenes; D005934:Glucagon / Q000031:analogs & derivatives; D008027:Light; D008099:Liver; D011956:Receptors, Cell Surface; D013050:Spectrometry, Fluorescence
D000596:Amino Acids; D001386:Azides; D005464:Fluorobenzenes; D011956:Receptors, Cell Surface; D005934:Glucagon