An improved method for extracting cell-free herpesviruses of Marek's disease and turkeys from infected cell cultures.
Sonic extraction of cell-free Marek's disease herpesvirus (MDHV) and turkey herpesvirus (HVT) from infected cell cultures was improved by incorporating sorbitol in the suspending media. Yields of cell-free virus of virulent MDHV were significantly increased with 10% sorbitol added to SPGA-EDTA buffer. Avirulent strains of MDHV and HVT were respectively readily extracted with SPGA-EDTA and SPGA as the suspending medium, and extraction of their cell-free viruses was moderately improved by adding sorbitol to the suspending medium.
D000818:Animals; D002474:Cell-Free System; D006564:Herpesviridae / Q000302:isolation & purification*; D008381:Herpesvirus 2, Gallid / Q000302:isolation & purification*; D008722:Methods; D013010:Sonication; D013012:Sorbitol; D014422:Turkeys / Q000382:microbiology
D013012:Sorbitol