Chromic-acid formaldehyde fixation of nucleic acids of bacteriophage phi6 and infectious bovine rhinotracheitis virus.
Bacteriophage phi6 nucleic acid was present as a torus after chromic acid-formaldehyde-OSO4 fixation and acetone and propylene oxide dehydration. A herpes virus, infectious bovine rhinotracheitis virus, had its DNA mostly as a torus, collapsed in the centre, or as a network, after glutaraldehyde-OSO4 fixation, but in an uncollapsed torus or network formation after chromic acid-formaldehyde-OSO4. This fixative stabilized nucleic acids, allowing acetone dehydration and plastic embedding without collapse of nucleic acid to the centre of the virion.
D002857:Chromium; D003090:Coliphages / Q000648:ultrastructure*; D004279:DNA, Viral / Q000032:analysis*; D005404:Fixatives; D005557:Formaldehyde; D005976:Glutaral; D007242:Herpesvirus 1, Bovine / Q000648:ultrastructure*; D009992:Osmium; D012367:RNA, Viral / Q000032:analysis*
D004279:DNA, Viral; D005404:Fixatives; D012367:RNA, Viral; D002857:Chromium; D005557:Formaldehyde; D009992:Osmium; D005976:Glutaral