Changes in enzyme activities during the artificially stimulated transition from follicular to luteal cell types in rat ovary.
This paper reports the time course of development of the intramitochondrial cholesterol side-chain-cleavage activity, cytoplasmic NADP+-dependent isocitric dehydrogenase, malic enzyme and glucose-6-phosphate dehydrogenase during ovarian maturation, using as a model the immature rat ovary stimulated to develop with pharmacological doses of gonadotrophin. These enzymic activities were correlated with increases in ovarian content of DNA, cellular content of adenosin 3':5'-monophosphate, and the levels of plasma progesterone. The plasma progesterone concentrations followed closely the development of the [4-14C]cholesterol side-chain-cleavage which was mimicked by the cytoplasmic isocitric dehydrogenase; both enzymes increased in activity 28 times during the 6 days of this study. There was no correlation between adenosine 3':5'-monophosphate levels and cholesterol side-chain cleavage or progesterone plasma concentrations.
D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000378:metabolism; D003338:Corpus Luteum / Q000502:physiology*; D000242:Cyclic AMP / Q000378:metabolism; D004971:Estrus / Q000187:drug effects; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D006064:Gonadotropins, Equine / Q000494:pharmacology*; D007521:Isocitrate Dehydrogenase / Q000378:metabolism; D008291:Malate Dehydrogenase / Q000378:metabolism; D008928:Mitochondria / Q000201:enzymology; D006080:Ovarian Follicle / Q000502:physiology*; D010053:Ovary / Q000187:drug effects / Q000201:enzymology*; D011247:Pregnancy; D011374:Progesterone / Q000378:metabolism; D051381:Rats
D006064:Gonadotropins, Equine; D011374:Progesterone; D000242:Cyclic AMP; D008291:Malate Dehydrogenase; D007521:Isocitrate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D002786:Cholesterol Side-Chain Cleavage Enzyme