[Histochemical and histoenzymatic studies of chick-embryo tibial rudiments cultured in vitro in the presence of bovine growth hormones].
The AA. have studied the effect of the bovine GH on the cellular and intercellular components of the chick embryo tibias cultured organotypically. They have observed a strong increase of the proliferative activity of the chondroblasts such as showed by a stronger uptake of the tritiated timidine by traited tibias in comparison with the control tibias. The chondroblasts of the traited tibias show an increase of some enzymatic activities connected with the aerobic cycle and of the UDPG-DH, connected with the uronic acids cycle. In the amorphous matrix of the traited tibias they have showed a greater polimerisation and sulfation of the condroitinesulfate A and C, which, almost exclusively, constitute the mucopolisaccharidic component of the matrix.
D000818:Animals; D002642:Chick Embryo; D003576:Electron Transport Complex IV / Q000032:analysis; D005966:Glucuronidase / Q000032:analysis; D013006:Growth Hormone / Q000494:pharmacology*; D010088:Oxidoreductases / Q000032:analysis; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis; D013977:Tibia / Q000196:embryology* / Q000201:enzymology
D013006:Growth Hormone; D010088:Oxidoreductases; D003576:Electron Transport Complex IV; D010744:Phosphoric Monoester Hydrolases; D005966:Glucuronidase
Richerche istochimiche ed istoenzimatiche su abbozzi tibiali di embrione di pollo coltivati "in vitro" in presenza di GH bovino