[Polypeptide synthetase activity of chromatin from eukaryotic cells].
It is shown that the polypeptide synthetase activity (PS-activity) of chromatin from rat liver is increased 9--21 hrs after partial hepatectomy. Among 9 amino acids studied alanine, methionine, lysine, tyrosine and arginine are not incorporated into the system in question. The highest rate of polymerization is observed in case of glutamic acid. The rate of glutamine, asparagine and glycine incorporation is 7--8 times slower. The PS-activity of chromatin is enhanced by chromatin preincubation with NAD (but not with its analogs). The activation is prevented by thymidine and nicotinamide. Storage of chromatin for 18 hrs at 2--4 degrees C results in a complete loss of PS-activity. Ability of "old" chromatin to incorporate of amino acids may be restored by its preincubation with NAD. Storage of chromatin in the presence of 5 mM cAMP does not decrease the PS-activity. It is assumed that in the system described poly-ADP ribose is an energy source for amino acid activation.
D049148:Aminoacylation; D000818:Animals; D001216:Asparagine / Q000378:metabolism; D002843:Chromatin / Q000201:enzymology*; D005971:Glutamates / Q000378:metabolism; D005973:Glutamine / Q000378:metabolism; D005998:Glycine / Q000378:metabolism; D006498:Hepatectomy; D008099:Liver / Q000201:enzymology*; D008297:Male; D009243:NAD / Q000378:metabolism; D009536:Niacinamide / Q000494:pharmacology; D010452:Peptide Biosynthesis; D011064:Poly Adenosine Diphosphate Ribose / Q000378:metabolism; D051381:Rats; D013936:Thymidine / Q000494:pharmacology
D002843:Chromatin; D005971:Glutamates; D005973:Glutamine; D009243:NAD; D009536:Niacinamide; D011064:Poly Adenosine Diphosphate Ribose; D001216:Asparagine; D005998:Glycine; D013936:Thymidine
Polipeptidsintetaznaia aktivnosy' khromatina kletok eukariotov.