Reversal of the unresponsiveness of neonatal rat ovary to LH in cAMP synthesis by estrogen.
The rat ovary during the 1st postnatal week is unresponsive to luteinizing hormone (LH), but responds to prostaglandin E1 with increase of cyclic adenosine 3',5'-monophosphate synthesis. In the present experiments unresponsiveness of ovaries of 6-day-old rats to LH in synthesis of cAMP was effectively reversed by injection of depot estradiol and diethylstilbestrol on the 2nd and 4th postnatal day. Administration of testosterone, progesterone, deoxycorticosterone, pregnant mare's serum gonadotropin and human chronic gonadotropin had no stimulatory effect. The lack of response to LH also failed to be reversed when estradiol was injected 21 h before killing of the animals or the ovaries were preincubated with estradiol. These results suggest that the development of an ovarian cell system responsive to LH in newborn rat may be accelerated by long-term action of estradiol.
D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D000242:Cyclic AMP / Q000096:biosynthesis*; D004054:Diethylstilbestrol / Q000494:pharmacology; D004357:Drug Synergism; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D066298:In Vitro Techniques; D007986:Luteinizing Hormone / Q000494:pharmacology*; D010053:Ovary / Q000187:drug effects*; D051381:Rats; D013997:Time Factors
D004958:Estradiol; D004054:Diethylstilbestrol; D007986:Luteinizing Hormone; D000242:Cyclic AMP