A rapid method for preparation of calf spleen exonuclease.
Chromatography on Concanavalin A sepharose is a simple procedure for partial purification of spleen phosphodiesterase. This procedure removes much, but not all, of the ribonuclease activity found as contaminant in most spleen phosphdisterase preparations.
D000818:Animals; D002417:Cattle; D002846:Chromatography, Affinity / Q000379:methods; D003208:Concanavalin A; D005092:Exonucleases / Q000302:isolation & purification*; D008970:Molecular Weight; D010727:Phosphoric Diester Hydrolases / Q000302:isolation & purification*; D012260:Ribonucleases / Q000302:isolation & purification*; D013154:Spleen / Q000201:enzymology*
D003208:Concanavalin A; D005092:Exonucleases; D012260:Ribonucleases; D010727:Phosphoric Diester Hydrolases