Effect of combined dopaminergic and GABA-ergic stimulation on ouabain-induced epileptiform activity.
In this study we tested a hypothesis that dopamine and GABA receptor-stimulating agents given together might mutually potentiate their anticonvulsant activities. To test this hypothesis, experiments were performed on free-moving rabbits with epileptiform activity evoked by cortical application of ouabain. After combined treatment with apomorphine and GHBA 3 out of 6 animals displayed no EEG epileptiform activity, and in the remaining 3 rabbits this activity was greatly reduced. A combined pretreatment with apomorphine and GHBA abolished epileptiform EEG activity during the first hour and shortened it during the second hour, but not significantly. Apomorphine given together with amino-oxyacetic acid before ouabain completely abolished the epileptiform activity in 4 out of 6 animals. In the remaining 2 rabbits the latency of ouabain affect was prolonged, the epileptiform activity during the first hour was abolished, and it was markedly decreased during the next 2 hr (significantly from controls or groups receiving apomorphine or amino-oxyacetic acid). Phenytoin was less potent than apomorphine with GHBA or apomorphine given together with AOAA. The animals' behavior was observed.
D000625:Aminooxyacetic Acid / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D001058:Apomorphine / Q000008:administration & dosage / Q000494:pharmacology*; D002540:Cerebral Cortex / Q000187:drug effects; D004338:Drug Combinations; D004357:Drug Synergism; D004569:Electroencephalography; D006885:Hydroxybutyrates / Q000008:administration & dosage / Q000494:pharmacology*; D008297:Male; D010042:Ouabain / Q000037:antagonists & inhibitors*; D010672:Phenytoin / Q000494:pharmacology*; D011817:Rabbits; D011954:Receptors, Dopamine / Q000187:drug effects*; D017981:Receptors, Neurotransmitter / Q000187:drug effects; D012640:Seizures / Q000139:chemically induced*; D005680:gamma-Aminobutyric Acid
D004338:Drug Combinations; D006885:Hydroxybutyrates; D011954:Receptors, Dopamine; D017981:Receptors, Neurotransmitter; D000625:Aminooxyacetic Acid; D005680:gamma-Aminobutyric Acid; D010042:Ouabain; D010672:Phenytoin; D001058:Apomorphine