Susceptibility of human embryonic kidneys in organ culture to herpesvirus hominis.
101 pairs of human embryonic kidneys of 5-12 weeks' gestation were maintained in whole organ culture by our previously described technique, with herpesvirus hominis types1 and 2 added to our regular media. One kidney of each pair served as a control and was exposed to identical culture medium without virus. Cultures were maintained for 24-120 h to study the time sequence of viral infectivity. Organs were then examined for the presence of virus by electron microscopy and histochemical staining. Histological studies of virus-infected kidneys showed either (1) complete organ death or (2) virus localization in undifferentiated cells, plus disorganization of architecture in differentiated areas. Control organs showed normal organization.
D003470:Culture Media; D003588:Cytopathogenic Effect, Viral; D005260:Female; D006801:Humans; D007181:Inclusion Bodies, Viral; D007668:Kidney / Q000196:embryology* / Q000382:microbiology / Q000473:pathology; D009924:Organ Culture Techniques; D011247:Pregnancy; D018139:Simplexvirus / Q000472:pathogenicity*
D003470:Culture Media