Effect of substance P and eledoisin on K+ efflux, amylase release and cyclic nucleotide levels in slices of rat parotid gland.
The undecapeptides, substance P and eledoisin, caused a rapid, concentration-dependent increase in K+ efflux and amylase release from parotid tissue slices. The effects were not blocked by beta-adrenergic, alpha-adrenergic, or cholinergic antagonists. Incubation buffer calcium was required for stimulation of K efflux and amylase release. The action of the undecapepides was independent of any effects on parotid cyclic AMP or cyclic GMP levels. Since the actions of the undecapeptides were Ca2+ dependent and no effects on cyclic nucleotide levels were discerned it was concluded that Ca2+ plays a primary role in agonist regulation of K+ efflux from the parotid.
D000681:Amylases / Q000378:metabolism*; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism*; D006152:Cyclic GMP / Q000378:metabolism*; D004600:Eledoisin / Q000494:pharmacology*; D005260:Female; D007700:Kinetics; D010306:Parotid Gland / Q000187:drug effects / Q000378:metabolism*; D010646:Phentolamine / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism*; D011433:Propranolol / Q000494:pharmacology; D051381:Rats; D013373:Substance P / Q000494:pharmacology*
D013373:Substance P; D004600:Eledoisin; D001285:Atropine; D011433:Propranolol; D000242:Cyclic AMP; D000681:Amylases; D006152:Cyclic GMP; D011188:Potassium; D010646:Phentolamine