The effect of induced metabolic acidosis on vitamin D3 metabolism in rachitic chicks.
The metabolism of vitamin D3 was studied in 3-week-old, vitamin D deficient chicks, fed since hatching with a diet containing 3% ammonium chloride, 1% calcium, and 0.7% phosphorus. When kidney homogenates were incubated in vitro with [3H]25-(OH)D3, the production of 1,25-(OH)2D3 was reduced by 40% in acidotic birds. During in vivo experiments, after injection of [3H]D3 (1220 pM/bird), the level of 1,25-(OH)2D3 was also reduced in blood plasma, intestine, and tibiae in acidotic chicks as compared with the controls. As a large increase in plasma phosphate was found during acidosis, these results are discussed in relation to the possible role of phosphorus in the control of 1,25-(OH)2D3 synthesis.
D000138:Acidosis / Q000139:chemically induced / Q000150:complications / Q000378:metabolism*; D000643:Ammonium Chloride / Q000009:adverse effects; D000818:Animals; D002645:Chickens; D002762:Cholecalciferol / Q000378:metabolism*; D004032:Diet; D004100:Dihydroxycholecalciferols / Q000096:biosynthesis / Q000097:blood; D006887:Hydroxycholecalciferols / Q000096:biosynthesis / Q000097:blood; D007421:Intestine, Small / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D012279:Rickets / Q000150:complications / Q000378:metabolism*; D013977:Tibia / Q000378:metabolism
D004100:Dihydroxycholecalciferols; D006887:Hydroxycholecalciferols; D000643:Ammonium Chloride; D002762:Cholecalciferol