[Distribution of enzymes in the stereocilia of the ductus epididymis of rats (author's transl)].
Using unfixed or cryostate sections from aldehyde fixed epididymides of adult rats gamma-glutamyl naphthylamides are hydrolysed in the stereocilia of the principal cells in zone 1A-4B and glycyl-prolyl naphthylamides in those of zone 1B-4A (nomenclature of Reid and Cleland, 1957); this is not the case when other amino acid and peptide naphthylamides are employed. Adenosine triphosphate (ATP) is metabolized by the stereocilia in zone 1a, 2, 3 and 4A and adenosine monophosphate (AMP) in the stereocilia of zone 3-6. With thiamine pyrophosphate (TPP) especially the Golgi apparatus of the principal cells in zone 1B and C, 2 and 3 reacts. In addition ATP, AMP and TPP stain the stereocilia of single epithelial cells outside the zones where all stereocilia react. The AMP pattern allows a further subdivision of zone 3 into 3 A and B. Substrates for the demonstration of alpha- and beta-D-glycosidases, non-specific esterases and alkaline phosphatases are either not hydrolyzed by the stereocilia or the rate of hydrolysis is low.
D000251:Adenosine Triphosphatases / Q000032:analysis; D000818:Animals; D002923:Cilia / Q000201:enzymology*; D004822:Epididymis / Q000201:enzymology* / Q000648:ultrastructure; D004950:Esterases / Q000032:analysis; D006026:Glycoside Hydrolases / Q000032:analysis; D006056:Golgi Apparatus / Q000201:enzymology; D008297:Male; D010447:Peptide Hydrolases / Q000032:analysis*; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis*; D051381:Rats; D013834:Thiamine Pyrophosphatase / Q000032:analysis
D004950:Esterases; D010744:Phosphoric Monoester Hydrolases; D006026:Glycoside Hydrolases; D010447:Peptide Hydrolases; D000251:Adenosine Triphosphatases; D013834:Thiamine Pyrophosphatase
Zur Verteilung der Stereocilienenzyme im Nebenhodengang von Ratten.