Enzymatic radioiodination of succinyl cyclic AMP tyrosine methyl ester by lactoperoxidase and radioimmunoassay for cyclic AMP.
A highly specific and simple radioimmunoassay for cyclic AMP with a sensitivity of 0.04 picomoles/tube has been developed according to the method of Steiner et al., using 125I-succinyl cyclic AMP tyrosine methyl ester as a tracer. The tracer with higher immunoactivities could be simply and constantly prepared by an enzymatic iodination procedure utilizing lactoperoxidase, radioactive iodide and hydrogen peroxide generated by glucose-glucose oxidase system, rather than by chloramine-T procedure.
D000273:Adipose Tissue / Q000032:analysis; D000818:Animals; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D000242:Cyclic AMP / Q000031:analogs & derivatives* / Q000032:analysis*; D007672:Kidney Cortex / Q000032:analysis; D007700:Kinetics; D007784:Lactoperoxidase; D008099:Liver / Q000032:analysis; D008297:Male; D008832:Microchemistry; D011863:Radioimmunoassay / Q000379:methods; D051381:Rats; D013737:Testis / Q000032:analysis
D000242:Cyclic AMP; D007784:Lactoperoxidase