Solid-phase radioimmunoassay for detecting bovine (neonatal calf diarrhea) rotavirus antibody.
An indirect solid-phase microradioimmunoassay is described for detecting antibodies against rotaviruses. The test involved ethanol fixation of microcultures of bovine rotavirus-infected BSC-1 cells and reacetion with bovine antirotavirus serum, followed by 125I-labeled rabbit anti-bovine immunoglobulin G. The technique was shown to be virus specific and highly sensitive. The fixed microcultures could be stored at 4 degrees C for at least 2 months without affecting the sensitivity of the test. The application of this system for the detection of rotavirus antibodies in humans is briefly discussed.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002417:Cattle; D001908:Diarrhea Viruses, Bovine Viral / Q000276:immunology*; D000431:Ethanol; D005404:Fixatives; D007074:Immunoglobulin G; D007457:Iodine Radioisotopes; D000432:Methanol; D008722:Methods; D012328:RNA Viruses / Q000276:immunology*; D011863:Radioimmunoassay; D012087:Reoviridae / Q000276:immunology
D000914:Antibodies, Viral; D005404:Fixatives; D007074:Immunoglobulin G; D007457:Iodine Radioisotopes; D000431:Ethanol; D000432:Methanol