DNA-binding proteins in mouse cells infected with polyoma virus.
DNA-binding proteins from uninfected or polyoma virus-infected mouse cells (3T6) were isolated and compared by DNA-cellulose chromatography and SDS-PAGE. During the early phase of infection, few but significant and reproducible differences were observed. In the 0.4 M NaCl eluate, four major new polypeptides were detected in infected cells, with approximate molecular weights of 160 K, 130 K, 86 K and 81 K, the last two being compatible with previous estimates for polyoma T antigen molecular weight.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D002845:Chromatography / Q000379:methods; D051379:Mice; D011120:Polyomavirus / Q000254:growth & development / Q000276:immunology / Q000378:metabolism*; D014176:Protein Biosynthesis; D014764:Viral Proteins / Q000096:biosynthesis*
D000956:Antigens, Viral; D014764:Viral Proteins