Micro determination of unsaturated disaccharide formed by the action of acidic glycosaminoglycan-endoeliminases. An application of the thiobarbituric acid method to the assay of D-gluco-4-enepyranosyluronic acid-containing disaccharides.
An improved method is described for the micro determination of acidic glycosaminoglycans after digestion with chondroitinase-ABC and -AC. The determination is based on the color production of D-gluco-4-enepyranosyluronic acid-containing disaccharides produced by the action of chondroitinase-ABC and -AC (acidic glycosaminoglycans-endoeliminase) when the periodate-thiobarbituric acid method is applied to the alpha,beta-unsaturated disaccharides. Suitable conditions for the quantitative assay are described.
D002809:Chondroitin Sulfates / Q000032:analysis; D002810:Chondroitinases and Chondroitin Lyases / Q000378:metabolism*; D004187:Disaccharides; D006025:Glycosaminoglycans / Q000032:analysis*; D006603:Hexuronic Acids / Q000032:analysis*; D008722:Methods; D010504:Periodic Acid; D010756:Phosphoric Acids; D013858:Thiobarbiturates; D014574:Uronic Acids / Q000032:analysis*
D004187:Disaccharides; D006025:Glycosaminoglycans; D006603:Hexuronic Acids; D010756:Phosphoric Acids; D013858:Thiobarbiturates; D014574:Uronic Acids; D010504:Periodic Acid; D002809:Chondroitin Sulfates; D002810:Chondroitinases and Chondroitin Lyases