Circular dichroic properties and conformation of thionicotinamide dinucleotides.
The spectroscopic properties of the 3-thioamide analogues of coenzymes NAD and NADH (sNAD and sNADH) have been investigated in order to obtain information about their conformational properties. In particular, ultraviolet absorption and circular dichroism properties of solutions in phosphate buffer pH 7 and ethanol were studied. Also equimolar mixtures of AMP and sNMN(H), obtained by cleaving the coenzymes with phosphodiesterase, were investigated using the same solvents. The appearance of a couplet around 260 nm, which is not present for the mixture of sNMN and AMP, suggests a stacking interaction of the two aromatic moieties in sNAD. This conclusion is further substantiated by a hyperchroism of the ultraviolet absorption band in the 260-nm region in both sNAD and sNADH. The comparison of the ultraviolet and circular dichroic properties of intact and cleaved coenzymes in the different solvent systems makes it possible to single out the bands which are more sensitive to conformation changes (i.e. to open-stacking equilibrium) and those which are sensitive to solvent effects only.
D000249:Adenosine Monophosphate; D002942:Circular Dichroism; D008968:Molecular Conformation; D009243:NAD / Q000031:analogs & derivatives*; D009537:Nicotinamide Mononucleotide; D010084:Oxidation-Reduction; D013056:Spectrophotometry, Ultraviolet; D013873:Thionucleotides
D013873:Thionucleotides; D009243:NAD; D009537:Nicotinamide Mononucleotide; D000249:Adenosine Monophosphate