Presence of free viral DNA in simian virus 40-transformed nonproducer cells.
Extracts from several simian virus 40 (SV40)-transformed nonproducer cells were prepared by the hot-phenol procedure normally used to extract cellular RNA. These extracts contained SV40 infectious units. Part of the infectious units were identified as SV40 form I DNA molecules. The results of reconstruction experiments suggest that SV40 form I DNA is extractable by the hot-phenol procedure because of its fast renaturation rate. The significance of the presence of free viral DNA in nonproducer transformed cells is discussed.
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D006224:Cricetinae; D004279:DNA, Viral / Q000032:analysis* / Q000302:isolation & purification; D008647:Mesocricetus; D008722:Methods; D013539:Simian virus 40 / Q000032:analysis* / Q000254:growth & development
D004279:DNA, Viral