title: Relationship between the methionine tryptic peptides of simian virus 40 and BK virus tumor antigens.
abstract: The monomer form of BK virus (BKV) tumor antigen (T Ag) was immunoprecipitated from extracts of BKV-transformed cells and had a molecular weight of approximately 113,000. This compared with 97,000 for the molecular weight of either BKV or simian virus 40 (SV40) T Ag from lytically infected cells. The SV40 and BKV T Ag's from productively infected cells were compared by examining their methionine-labeled tryptic peptides. Out of a total of 20 SV40-and 21 BKV-specific peptides, there were seven pairs of similar peptides on the basis of ion-exchange chromatography, These coeluting peptides contained approximately 25 to 30% of the total methionine radioactivity. Similar results were obtained when the tryptic peptides of SV40 T Ag from lytically infected cells were compared with those of BKV T Ag from virally transformed cells.
mesh_terms: D000951:Antigens, Neoplasm / Q000032:analysis*; D000956:Antigens, Viral / Q000032:analysis*; D001739:BK Virus / Q000276:immunology*; D002460:Cell Line; D002472:Cell Transformation, Viral; D008715:Methionine / Q000032:analysis; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis*; D011120:Polyomavirus / Q000276:immunology*; D011487:Protein Conformation; D013539:Simian virus 40 / Q000276:immunology*
chemical_list: D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D010455:Peptides; D008715:Methionine