[Differences in transcription of nuclear RNA from rat liver with normal and malignant growth].
Total nuclear RNA was isolated under conditions of actinomycin D blocking ion-exchange chromatography on kieselguhr columns with methylated albumin detected differences in transpiration of rat liver nuclear RNA with intensive normal and malignant growth. Actinomycin D in doses blocking the appearance of peculiar proteins in the blood serum of rats with the regenerating liver and RS-1 hepatoma produces a different effect on the biosynthesis of nuclear DNA-like RNA of the rat liver. 24h after a partial hepatectomy the antibiotic inhibits considerably the biosynthesis of nuclear DNA-like RNA which is eluated during chromatographying with 0.2% solution of sodium dodecyl sulphate at 70 degrees C. With RS-1 hepatoma the actinomycin D effect is most pronounced with respect to nuclear DNA-like RNA of rats with a tumour which is washed off from the column with a 0.2% solution of sodium dodecyl sulphate at 37 degrees C.
D000818:Animals; D001798:Blood Proteins / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D003609:Dactinomycin / Q000494:pharmacology; D006498:Hepatectomy; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008113:Liver Neoplasms / Q000378:metabolism*; D008115:Liver Regeneration; D009374:Neoplasms, Experimental / Q000378:metabolism; D012313:RNA / Q000096:biosynthesis*; D051381:Rats; D014158:Transcription, Genetic
D001798:Blood Proteins; D003609:Dactinomycin; D012313:RNA
Vidminnosti u transkriptsii iadernikh RNK pechinki shchuriv za normal'nogo i zloiakisnogo rostu.