Isolation and characterization of lipoprotein-X from the pig.
In vitro incubation of pig bile with pig whole serum resulted in the formation of "LP-X like" material migrating towards the cathode on agar-gel electrophoresis. As in all other species studied so far, LP-X occurred also in the serum of bile duct ligated pigs, however, relatively late (48-72h) after the operation. In parallel, alterations of some serum parameters and of the protein-lipid composition of the different lipoporotein fractions were observed. While the electrophoretic behaviour, the protein-lipid and apoprotein composition of pig LP-X were comparable to those found in man, dog and rat, it sedimated in most instances at the density 1.063 g/ml.
D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D001663:Bilirubin / Q000097:blood; D002784:Cholesterol / Q000097:blood; D004587:Electrophoresis, Agar Gel; D005260:Female; D008074:Lipoproteins / Q000097:blood / Q000302:isolation & purification; D008075:Lipoproteins, HDL / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood; D008079:Lipoproteins, VLDL / Q000097:blood; D008297:Male; D008854:Microscopy, Electron; D008970:Molecular Weight; D010743:Phospholipids / Q000097:blood; D013552:Swine; D014280:Triglycerides / Q000097:blood
D008074:Lipoproteins; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D010743:Phospholipids; D014280:Triglycerides; D002784:Cholesterol; D000469:Alkaline Phosphatase; D001663:Bilirubin