title: Bovine leukemia virus-associated antigens in lymphocyte cultures.
abstract: Short-term lymphocyte cultures from bovine leukemia virus (BLV)-infected cattle were tested for BLV-associated antigens at various times after incubation. Several immunologic methods were used, including fluorescent antibody tests, immunodiffusion, and radial immunodiffusion. Antigens were not detected in uncultured lymphocytes. The BLV-associated antigens were detected as early as 3 hours, with maximum antigen production occurring at 18 to 24 hours after incubation. These results indicate that culturing of lymphocytes in vitro is necessary for the expression of the virus.
mesh_terms: D000818:Animals; D000956:Antigens, Viral; D002417:Cattle; D002478:Cells, Cultured; D005455:Fluorescent Antibody Technique; D005779:Immunodiffusion; D001909:Leukemia Virus, Bovine / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology*; D012190:Retroviridae / Q000276:immunology*
chemical_list: D000956:Antigens, Viral