Reversed-phase, high-pressure liquid chromatography of peptides and proteins with ion-pairing reagents.
Reversed-phase, high-pressure liquid chromatography has been successfully applied to the analysis of peptides and proteins by the addition of hydrophilic (for example, phosphoric acid) or hydrophobic (for example, hexanesulfonic acid) ion-pairing reagents, or both, to the mobile phase. Examples described included proteins such as insulin, glucagon, and 1-24 ACTH pentaacetate (ACTH is adrenocorticotrophic hormone).
D000324:Adrenocorticotropic Hormone / Q000302:isolation & purification; D002352:Carrier Proteins / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005934:Glucagon / Q000302:isolation & purification; D007328:Insulin / Q000302:isolation & purification; D009842:Oligopeptides / Q000302:isolation & purification*; D010446:Peptide Fragments / Q000302:isolation & purification*; D010710:Phosphates; D011506:Proteins / Q000302:isolation & purification*; D012997:Solvents
D002352:Carrier Proteins; D007328:Insulin; D009842:Oligopeptides; D010446:Peptide Fragments; D010710:Phosphates; D011506:Proteins; D012997:Solvents; D000324:Adrenocorticotropic Hormone; D005934:Glucagon