Inhibition by Mg2+ of the interaction of Ca2+ with spin-labeled g2 bound to myosin.
According to the measurement of ESR spectrum, Ca2+ induced conformational change of spin-labeled g2 bound to myosin in the presence of 1 mM Mg2+. The half-maximal changes were observed at pCa 6.8 and at pCa 3.7. Spin-labeled phosphorylated g2 bound to myosin showed one transition at pCa 4.5, which shifted to pCa 6.5 after the dephosphorylation  with E. coli alkaliphosphatase.
D002118:Calcium / Q000494:pharmacology*; D004578:Electron Spin Resonance Spectroscopy; D066298:In Vitro Techniques; D008274:Magnesium / Q000494:pharmacology*; D009218:Myosins / Q000378:metabolism; D013113:Spin Labels
D013113:Spin Labels; D009218:Myosins; D008274:Magnesium; D002118:Calcium