Defined dimensional changes in enzyme cofactors: fluorescent "stretched-out" analogs of adenine nucleotides.
A concept is presented for testing the dimensional restrictions of enzyme-active sites by stretching the substrate or cofactor by known magnitude. These restrictions of enzyme-active sites specific for purine cofactors were tested by the synthesis and evaluation of lin-benzoadenosine 5'-triphosphate, 5'-diphosphate, and 3',5'-monophosphate with respect to enzyme binding and activity. These "stretchedout" (by 2.4 angstroms) versions of the adenine ribonucleotides bind strongly, slow the enzymatic rates, and have useful fluorescence properties.
D000227:Adenine Nucleotides / Q000378:metabolism*; D000244:Adenosine Diphosphate / Q000031:analogs & derivatives; D000255:Adenosine Triphosphate / Q000031:analogs & derivatives; D000818:Animals; D001665:Binding Sites; D002384:Catalysis; D055598:Chemical Phenomena; D002621:Chemistry; D003067:Coenzymes / Q000378:metabolism*; D006593:Hexokinase / Q000378:metabolism; D066298:In Vitro Techniques; D010732:Phosphofructokinase-1 / Q000378:metabolism; D010735:Phosphoglycerate Kinase / Q000378:metabolism; D010770:Phosphotransferases / Q000378:metabolism; D011117:Polyribonucleotide Nucleotidyltransferase / Q000378:metabolism; D011485:Protein Binding; D011770:Pyruvate Kinase / Q000378:metabolism; D011817:Rabbits; D012441:Saccharomyces cerevisiae; D013050:Spectrometry, Fluorescence; D013329:Structure-Activity Relationship
D000227:Adenine Nucleotides; D003067:Coenzymes; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D010770:Phosphotransferases; D006593:Hexokinase; D010732:Phosphofructokinase-1; D011770:Pyruvate Kinase; D010735:Phosphoglycerate Kinase; D011117:Polyribonucleotide Nucleotidyltransferase