Detection and evaluation of feline oncornavirus-induced cell surface antigen(s) shed from cells in vitro.
A method for preparation of soluble feline oncornavirus-induced cell surface antigens was described. This technique relied upon the natural release of antigen(s) from FL-74 feline lymphoblastoid cells during their maintenance at 37 degrees in serum-deficient medium. When concentrated and clarified spent medium from 4-day cultures was tested for its antigen content by inhibition of humoral cytotoxicity, it was found that this  natural production of soluble antigen provided more feline oncornavirus-associated cell membrane antigen per cell than did a solubilization procedure in which papain was used. The shed antigen preparation was immunogenic in cats, eliciting humoral antibody that was reactive with the surface of FL-74 Cells and feline sarcoma virus-transformed nonproducer mink cells but was not reactive with feline leukemia virus in a virus neutralization assay.
D000818:Animals; D000912:Antibodies, Neoplasm / Q000096:biosynthesis; D000914:Antibodies, Viral / Q000096:biosynthesis; D000951:Antigens, Neoplasm / Q000008:administration & dosage / Q000302:isolation & purification*; D000956:Antigens, Viral / Q000008:administration & dosage / Q000302:isolation & purification*; D002415:Cats; D002462:Cell Membrane / Q000276:immunology; D002478:Cells, Cultured; D005256:Leukemia Virus, Feline / Q000276:immunology*; D007942:Leukemia, Experimental / Q000276:immunology*; D012995:Solubility; D014412:Tumor Virus Infections / Q000276:immunology*
D000912:Antibodies, Neoplasm; D000914:Antibodies, Viral; D000951:Antigens, Neoplasm; D000956:Antigens, Viral