Apparent microviscosity of intact and post-lipolysis ("remnant") very low density lipoprotein particles.
The apparent microviscosity of intact rat plasma very low density lipoprotein (VLDL) and post-lipolysis very low density lipoprotein was determined by fluorescence depolarization measurements and flurorescence decay measurements using 1, 6-diphenylhexatriene. Post-lipolysis very low density lipoprotein was prepared in vitro after incubation of the intact lipoprotein with either purified bovine milk lipoprotein lipase or lipoprotein lipase rich (post-heparin) plasma. During lipolysis, an average of 88% of the triglycerides were hydrolyzed, and the lipoprotein became depleted in phospholipids, cholesterol and apolipoprotein C. The apparent microviscosity of the lipoprotein increased by three-fold from 0.63 to 1.88 poise. It is concluded that the compositional changes occurring during lipolysis affect the physical properties of the lipoprotein, as measured here by the fluidity (microviscosity) of the particles.
D000818:Animals; D002417:Cattle; D005454:Fluorescence Polarization; D008071:Lipoprotein Lipase; D008079:Lipoproteins, VLDL / Q000097:blood; D008297:Male; D008892:Milk / Q000201:enzymology; D011487:Protein Conformation; D051381:Rats; D014783:Viscosity
D008079:Lipoproteins, VLDL; D008071:Lipoprotein Lipase