An investigation into the relationship of the immunological status of Herpes simplex virus type-2 (HSV-2) infected animals and the tumor-enhancing property of this infection.
In order to compare the state of immunity of HSV-2 infected animals with their sham treated counterparts, several in vivo methods for cell-mediated immunity in addition to the stimulation in vitro of mouse splenocytes with PHA were performed. The grafting of C 3 H mice with heterologous skin and the paw test with PHA showed no noticeable difference between the two groups. The tuberculin test performed on guinea pigs infected via the footpads, permitted the detection of a slightly impaired reactivity of the virus-infected animals. The PHA assay in vitro showed reduced stimulation indices with cultures of splenocytes from virus-infected mice about 10 days p.i. These reduced stimulation indices are the result of 1. a low 3 HTdR incorporation by the PHA stimulated cultures and 2. of a relatively high 3 HTdR uptake by the unstimulated control cultures. An interpretation of these results is suggested. Finally, the possible implications of a shortlived impaired cell-mediated immunity on the tumour enhancing property of the HSV-2 infection, which has been described in previous publications, are discussed.
D000818:Animals; D000912:Antibodies, Neoplasm / Q000302:isolation & purification; D000914:Antibodies, Viral / Q000302:isolation & purification; D000917:Antibody Formation / Q000187:drug effects; D005260:Female; D006168:Guinea Pigs; D000882:Haplorhini; D006801:Humans; D007111:Immunity, Cellular / Q000187:drug effects; D037102:Lectins / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D018139:Simplexvirus / Q000276:immunology*; D002583:Uterine Cervical Neoplasms / Q000276:immunology*
D000912:Antibodies, Neoplasm; D000914:Antibodies, Viral; D037102:Lectins