Cross-linking of ubiquinone cytochrome c reductase (complex III) with periodate-cleavable bifunctional reagents.
Two novel cross-linkers, disuccinimidyl tartarate (DST) and N,N'-bis(3-succinimidyloxycarbonylpropyl)tartaramide (SPT), have been synthesized. These reagents span 6 and 18 A, respectively, between functional groups and contain a vic-glycol bond which can be cleaved with periodate under mild reaction conditions. Both DST and SPT have been used to examine the near-neighbor relationships of polypeptides in ubiquinone cytochrome c reductase (complex III) from beef heart mitochondria. Among the cross-linked products resolved were pairs containing I + II, II + VI, I + V, and VI + VII. Polypeptides III and IV, a cytochrome b aproprotein, and the cytochrome c1 hemoprotein, respectively, were also resolved in several cross-linked products.
D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D003574:Cytochrome c Group; D046911:Macromolecular Substances; D008929:Mitochondria, Heart / Q000201:enzymology; D008970:Molecular Weight; D009097:Multienzyme Complexes; D009247:NADH, NADPH Oxidoreductases; D011808:Quinone Reductases; D013388:Succinimides; D013644:Tartrates
D003574:Cytochrome c Group; D046911:Macromolecular Substances; D009097:Multienzyme Complexes; D013388:Succinimides; D013644:Tartrates; C018052:disuccinimidyl tartarate; C018006:N,N'-bis(3-succinimidyloxycarbonylpropyl)tartaramide; D009247:NADH, NADPH Oxidoreductases; D011808:Quinone Reductases