Rapid biochemical tests for characterization of the Mycoplasmatales.
Methods are described for the rapid detection of beta-D-glucosidase and phosphatase in mycoplasma cultures using fluorogenic 4-methylumbelliferone substrates. These methods were applied to a selection of mycoplasma cultures and were compared with the conventionally used tests for these enzymes. Results were similar by both methods, but the fluorogenic tests could be read after 1 h, whereas the conventional tests took several days.
D000127:Acholeplasma / Q000201:enzymology / Q000378:metabolism; D001431:Bacteriological Techniques; D005069:Evaluation Studies as Topic; D005453:Fluorescence; D005959:Glucosidases / Q000096:biosynthesis*; D006868:Hydrolysis; D006923:Hymecromone / Q000378:metabolism; D009174:Mycoplasma / Q000201:enzymology / Q000378:metabolism; D009179:Mycoplasmatales / Q000201:enzymology*; D010744:Phosphoric Monoester Hydrolases / Q000096:biosynthesis*; D013045:Species Specificity
D006923:Hymecromone; D010744:Phosphoric Monoester Hydrolases; D005959:Glucosidases