Physical map of the Kirsten sarcoma virus genome as determined by fingerprinting RNase T1-resistant oligonucleotides.
From analysis of the large RNase T1-resistant oligonucleotides of Kirsten sarcoma virus (Ki-SV), a physical map of the virus genome was deduced. Kirsten murine leukemia virus (Ki-MuLV) sequences were detected in T1 oligonucleotides closest to the 3' end of the viral RNA and extended approximately 1,000 nucleotides into the genome. The rat genetic sequences started at this point and extended all the way to the very 5' end of the RNA molecules, where a small stretch of Ki-MuLV sequence was detected. By comparison of the fingerprints of Ki-SV RNA and the RNA of the endogenous rat src genetic sequences, it was found that more than 50% of the T1 oligonucleotides were similar between Ki-SV and the endogenous rat src RNA, suggesting an identical primary nucleotide sequence in over 50% of the viral genomes. The results indicate that Ki-SV arose by recombination between the 5' and 3' ends of Ki-MuLV and a large portion of the homologous sequences of the endogenous rat src RNA.
D001483:Base Sequence; D017988:Gammaretrovirus / Q000032:analysis* / Q000235:genetics; D005814:Genes, Viral; D006378:Helper Viruses / Q000032:analysis; D009052:Leukemia Virus, Murine / Q000032:analysis; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000032:analysis*; D012367:RNA, Viral / Q000032:analysis*; D012260:Ribonucleases / Q000378:metabolism
D009841:Oligonucleotides; D012367:RNA, Viral; D012260:Ribonucleases