Preparation of plates with a permanent adsorbent layer and their application in the analytical thin-layer chromatography of lipids.
Experimental conditions for preparing the thin-layer chromatographic plates with a permanent adsorbent layer (PAL) have been established; the particles of this layer are firmly bound to each other and to the glass support by means of fused glass powder. To prepare the PAL plates, a mixture of Woelm silica gel and glass powder (1:3, w/w) of particle size 7+/-1 mum was suspended in toluene and spread on the plates, yielding PALs of thickens 150-250 mum. The plates were heated in an electric furnace at 675 degrees for 20 min. In the separation of neutral lipids, a PAL thus prepared is equal in efficiency and selectivity to silica gel layers prepared in the usual manner.
D000327:Adsorption; D002855:Chromatography, Thin Layer; D008055:Lipids / Q000032:analysis*; D008722:Methods; D012822:Silicon Dioxide
D008055:Lipids; D012822:Silicon Dioxide