title: Influence of dibutyryl cyclic AMP on thymidine uptake by herpes simplex virus infected cells and the intracellular level of cyclic AMP.
abstract: Dibutyryl cyclic AMP inhibits the increase of dThd and BrdUrd transport normally observed after infection with Herpesvirus hominis, type I and II. Incorporation is also reduced. Inhibition of uptake is non-competitive as analysed by the Lineweaver-Burk plot. Addition of this drug to infected cells also reduces the activity of the thymidine kinase (EC 2.7.1.75). Transport of dUrd, dCyd and dAdo is not reduced. 4--8 h after infection with thymidine kinase (+) herpes strains the level of cAMP increases. On infection with a thymidine kinase (-) virus, only a small elevation  of cAMP can be shown. It was also found that early addition of actinomycin D or of cycloheximide prevents the increase of the cAMP level. This increase seems to depend on the activity of the herpes genome, because ultraviolet irradiation of infective particles destroys this ability.
mesh_terms: D001692:Biological Transport; D001973:Bromodeoxyuridine / Q000378:metabolism; D003994:Bucladesine / Q000494:pharmacology*; D002460:Cell Line; D000242:Cyclic AMP / Q000378:metabolism*; D003513:Cycloheximide / Q000494:pharmacology; D004261:DNA Replication / Q000187:drug effects; D004279:DNA, Viral / Q000096:biosynthesis; D003609:Dactinomycin / Q000494:pharmacology; D003853:Deoxyribonucleosides / Q000378:metabolism; D007700:Kinetics; D018139:Simplexvirus / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D013045:Species Specificity; D013936:Thymidine / Q000378:metabolism*; D013937:Thymidine Kinase / Q000378:metabolism; D013942:Thymine Nucleotides / Q000378:metabolism; D014466:Ultraviolet Rays; D014779:Virus Replication / Q000187:drug effects
chemical_list: D004279:DNA, Viral; D003853:Deoxyribonucleosides; D013942:Thymine Nucleotides; D003609:Dactinomycin; D003994:Bucladesine; D003513:Cycloheximide; D000242:Cyclic AMP; D013937:Thymidine Kinase; D001973:Bromodeoxyuridine; D013936:Thymidine