[Detection of multiple forms of polyphosphate phosphohydrolase from Endomyces magnusii].
Labile polyphosphate phosphohydrolase from Endomyces magnusii is 27-fold purified by means of fractionation with ammonium sulphate, gel filtration on Sephadex G-75 and Biogel P-60 and chromatography on DEAE cellulose. Chromatography on DEAE Sephadex A-50, isoelelctric focusing and polyacrylamide gel electrophoresis of the enzyme preparation revealed 3 different fractions with polyphosphate phosphohydrolase activity (PPPH1, PPPH2 and PPPH3). Relative content of these fractions in E. magnusii cells is 30%, 55% and 15% respectively. Isoelectric points are: PPPH1--pH 5.1--5.2; PPPH2--pH 6.0--6.1; PPPH3--pH 6.3--6.4. PPPH1 and PPPH2 are found to be the most labile. PPPH3 is more stable under isolation procedure and storage. The fractions have similar molecular weight (48 000 +/- 3000).
D001203:Ascomycota / Q000201:enzymology*; D002850:Chromatography, Gel / Q000379:methods; D002852:Chromatography, Ion Exchange; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D007525:Isoelectric Focusing; D008970:Molecular Weight; D010710:Phosphates; D010744:Phosphoric Monoester Hydrolases / Q000302:isolation & purification*; D004718:Saccharomycetales / Q000201:enzymology*
D003470:Culture Media; D010710:Phosphates; D010744:Phosphoric Monoester Hydrolases
Obnaruzhenie mnozhestvennosti form polifosfat-fosfogidrolazy Endomyces magnusii.