title: Nucleotide sequence of the restriction fragment Hind-F-EcoRI1 of simian-virus-40 DNA (part of the VP1 gene).
abstract: The nucleotide sequence of the simian virus 40 (SV40) genome region between the cleavage sites for restriction endonucleases EcoRI (map position 0) and HindII (map position 0.05) has been determined mainly by the partial chemical DNA degradation procedure of Maxam and Gilbert. This fragment represents 5.3% of the genome of SV40 and is located in the late region, internally in the VP1 gene. The message strand shows only one open reading frame for translation into protein, which connects to the one for the preceding fragment. On this basis part of the amino acid sequence of the VP1 protein is presented.
mesh_terms: D001483:Base Sequence; D003062:Codon; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004279:DNA, Viral / Q000032:analysis*; D004926:Escherichia coli / Q000201:enzymology; D005814:Genes, Viral; D009690:Nucleic Acid Conformation; D012333:RNA, Messenger / Q000032:analysis; D013539:Simian virus 40 / Q000032:analysis*; D014764:Viral Proteins / Q000096:biosynthesis
chemical_list: D003062:Codon; D004279:DNA, Viral; D012333:RNA, Messenger; D014764:Viral Proteins; D004262:DNA Restriction Enzymes