A technique for isolating turkey respiratory adenoviruses.
Several turkey respiratory adenoviruses were isolated in turkey embryonic liver cells from nasal turbinate filtrate collected from clinically ill turkey poults during postmortem examination. Suspect adenovirus inoculum had to remain in cell culture for 5 to 7 days, and 9 blind passages were required before a sample was declared negative. Once isolated, the turkey adenoviruses adapted rapidly to turkey kidney cells.
D000256:Adenoviridae / Q000302:isolation & purification*; D000257:Adenoviridae Infections / Q000382:microbiology / Q000662:veterinary*; D000818:Animals; D000259:Aviadenovirus / Q000254:growth & development / Q000276:immunology / Q000302:isolation & purification*; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D011201:Poultry Diseases / Q000382:microbiology*; D012141:Respiratory Tract Infections / Q000382:microbiology / Q000662:veterinary; D014422:Turkeys