"Fingerprinting" high molecular weight RNA by two-dimensional gel electrophoresis: application to poliovirus RNA.
Conditions are described under which complete RNase T1 digests of high molecular weight RNA can be separated into numerous components by two-dimensional gel electrophoresis. Small and large oligonucleotides (n = 1 - 2c0) can be resolved without losses. The procedure yields fingerprints which are diagnostic for a particular species of RNA and an index of its purity as will be shown for the genomes of poliovirus type 1 and 2.
D004591:Electrophoresis, Polyacrylamide Gel / Q000295:instrumentation; D008970:Molecular Weight; D009843:Oligoribonucleotides / Q000032:analysis; D017955:Poliovirus / Q000032:analysis*; D012367:RNA, Viral; D006163:Ribonuclease T1; D012260:Ribonucleases; D013045:Species Specificity
D009843:Oligoribonucleotides; D012367:RNA, Viral; D012260:Ribonucleases; D006163:Ribonuclease T1