Routine diagnosis of human rotaviruses in stools.
Electron microscopy, immune electron microscopy and complement fixation as methods of detecting rotavirus in the stools of young children with gastroenteritis were compared in a blind study during the winter of 1975-6. Complement fixation was the simplest to perform, was as sensitive as the other two, and allowed a quantitative measurement of viral excretion. Absorption of faecal extracts with fetal calf serum usually removed the anticomplementary activity of faecal extracts.
D002648:Child; D003168:Complement Fixation Tests; D003967:Diarrhea / Q000209:etiology; D005243:Feces / Q000382:microbiology*; D006801:Humans; D012328:RNA Viruses / Q000302:isolation & purification*; D012401:Rotavirus / Q000302:isolation & purification* / Q000648:ultrastructure; D014777:Virus Diseases / Q000175:diagnosis*