title: Effect of adenosine and catecholamines on cyclic AMP levels in guinea pig heart.
abstract: Adenosine has been shown to stimulate cyclic AMP accumulation in guinea pig atrial and ventricular slice preparations in a rapid and dose-dependent manner. Response to adenosine was inhibited by therophylline, potentiated by several other phosphodiesterase inhibitors and unaffected by alpha- or beta-adrenergic blocking agents. Agonist action required free hydroxyl groups at 2', 3', and 5' positions on the ribosyl moiety, retention of one proton on the 6-amino group, N at position 7, and unsubstituted C at position 8 on the purine moiety. The combined action between adenosine and isoproterenol was the sum of the individual stimulations. The alpha-agonist action of epinephrine and norepinephrine reduced cyclic AMP levels stimulated by beta-agonist or by adenosine; this action was mimicked by methoxamine. The effect of an alpha-agonist was particularly pronounced when cyclic AMP levels were elevated by adenosine.
mesh_terms: D000241:Adenosine / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D001665:Binding Sites; D002395:Catecholamines / Q000494:pharmacology*; D000242:Cyclic AMP / Q000378:metabolism*; D004347:Drug Interactions; D005260:Female; D006168:Guinea Pigs; D066298:In Vitro Techniques; D009206:Myocardium / Q000378:metabolism*; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology; D013329:Structure-Activity Relationship
chemical_list: D002395:Catecholamines; D010726:Phosphodiesterase Inhibitors; D000242:Cyclic AMP; D000241:Adenosine