[Operon study of riboflavin biosynthesis in Bacillus subtilis. XII. The determination of the ATP:riboflavin-5'-phosphotransferase and riboflavinsynthetase content in the cells with varying genotypes].
Activities of riboflavinkinase and riboflavinsynthetase were measured in 15 strains of Bacillus subtilis with different genotype. The increased level of riboflavinkinase was observed in strains, resistant to lumiflavin or lumichrome. Specific activity of riboflavinkinase was found to be about 100 times lower than that of riboflavinsynthetase. The regulation of biosynthesis of these enzymes seems to proceed non-coordinately. This phenomenon can be the sequence of the existence of many operators, controlling the flavinogenesis in Bac. subtilis.
D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism; D002474:Cell-Free System; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation; D005182:Flavin-Adenine Dinucleotide / Q000096:biosynthesis; D005838:Genotype; D009876:Operon; D010770:Phosphotransferases / Q000235:genetics*; D012256:Riboflavin / Q000096:biosynthesis*; D012258:Riboflavin Synthase / Q000235:genetics*; D014166:Transferases / Q000235:genetics*
D005182:Flavin-Adenine Dinucleotide; D014166:Transferases; D012258:Riboflavin Synthase; D010770:Phosphotransferases; D012256:Riboflavin
Issledovanie operona biosinteza riboflavina u Bacillus subtilis. Soobshchenie XII. Opredelenie soderzhaniia ATF:riboflavin-5'-fosfotransferazy i riboflavinsintetazy v kletakh s razlichnym genotipom.