The effects of herpes simplex virus type 1 on cellular DNA-dependent RNA polymerase activities.
An investigation of the activity of nuclear RNA polymerase following infection of LS cells with HSV-1 shows a decline in both major activities. This effect is not entirely due to inhibition of cellular protein synthesis, and the effect of alpha-amanitin-sensitive RNA polymerase is mediated by a protein(s) synthesized in the infected cell. Changes in the properties of this RNA polymerase activity include a reduction in the relative UTP/GTP incorporation ratio and an increased sensitivity to inhibition by actinomycin D, indicating that RNA polymerase II is involved in virus transcription.
D000546:Amanitins / Q000494:pharmacology; D002460:Cell Line; D002467:Cell Nucleus / Q000201:enzymology*; D003513:Cycloheximide / Q000494:pharmacology; D004279:DNA, Viral / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D003609:Dactinomycin / Q000494:pharmacology; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D009994:Osmolar Concentration; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis; D012367:RNA, Viral / Q000096:biosynthesis; D018139:Simplexvirus / Q000254:growth & development; D014158:Transcription, Genetic
D000546:Amanitins; D004279:DNA, Viral; D012367:RNA, Viral; D003609:Dactinomycin; D012313:RNA; D003513:Cycloheximide; D012321:DNA-Directed RNA Polymerases; D008274:Magnesium