[Formation of pseudotypes of VSV after culture in a human melanoma].
Electron microscope studies showed a high production of melanosomes and viral particles budding into the cisternae of the endoplasmic reticulum in cells derived from a subcutaneous metastasis. The history of this subline (HM6B-A) has been summarized elsewhere. An amelanotic subline (provided by the same patient) did not show similar virus particles. When infected with a vesicular stomatitis virus (VSV) thermolabile mutant (tl), these cells produced a VSV pseudotype in which a thermosensitive antigen was modified. Modification of surface VSV antigens was also detected by neutralisation tests. Using these tests, the VSV-pseudotype particles could be used as a tool to detect one or more antigens to a "putative" human melanomavirus, which might be only partially expressed.
D000956:Antigens, Viral; D002478:Cells, Cultured; D006801:Humans; D007065:Idoxuridine / Q000494:pharmacology; D007181:Inclusion Bodies, Viral; D008545:Melanoma / Q000209:etiology / Q000382:microbiology; D014721:Vesicular stomatitis Indiana virus; D014771:Virion; D014776:Virus Cultivation
D000956:Antigens, Viral; D007065:Idoxuridine
Formation de pseudotypes de VSV après culture dans un mélanome humain.