Mode of interaction of different polyanions with the first (C1,C1) the second (C2) and the fourth (C4) component of complement. IV. Activation of C1 in serum by polyanions.
Treatment of serum with dextransulphate polyvinylsulphate or polyanetholsulphonate resulted in a dose-dependent activation of C1 and C3; this was found for normal serum as well as for C4-deficient guinea-pig serum. Activation of C1 and C3 occurred at the same concentration of polyanions. The consumption of C3 in C4 deficient serum and the requirement of factor D of the alternative pathway indicate that C3 is activated via the alternative pathway.
D000818:Animals; D000838:Anions / Q000494:pharmacology*; D003172:Complement C1; D003175:Complement C2; D003176:Complement C3 / Q000096:biosynthesis; D003181:Complement C4; D003165:Complement System Proteins; D003911:Dextrans / Q000494:pharmacology; D006168:Guinea Pigs / Q000276:immunology; D006461:Hemolysis; D066298:In Vitro Techniques; D011074:Polyanetholesulfonate / Q000494:pharmacology; D011145:Polyvinyls / Q000494:pharmacology
D000838:Anions; D003172:Complement C1; D003175:Complement C2; D003176:Complement C3; D003181:Complement C4; D003911:Dextrans; D011145:Polyvinyls; D011074:Polyanetholesulfonate; D003165:Complement System Proteins