title: Isolation of feline leukemia virus from clinical specimens.
abstract: Specimens obtained from feline leukemia virus (FeLV)-positive cats were examined for infectious FeLV. Feline leukemia virus was detected by a focus-forming assay and confirmed by florescent antibody. Techniques of sample processing were evaluated and adjusted for optimum detection of FeLV. Low levels of FeLV were detected in 2 of 10 oral samples; however, the majority of these samples (17 of 27 tested) produced cytopathic effects in tissue culture which prevented Fe LV detection. Three of 24 urine samples and 1 of 20 rectal specimens were positive for FeLV. One milk sample contained high levels of FeLV.
mesh_terms: D000818:Animals; D001769:Blood / Q000382:microbiology; D002371:Cat Diseases / Q000382:microbiology*; D002415:Cats; D003588:Cytopathogenic Effect, Viral; D005260:Female; D005256:Leukemia Virus, Feline / Q000254:growth & development / Q000276:immunology / Q000302:isolation & purification*; D008297:Male; D008892:Milk / Q000382:microbiology; D009055:Mouth / Q000382:microbiology; D014556:Urine / Q000382:microbiology