Effects of estrogen, glucocorticoid, glucagon, and adenosine 3':5'-monophosphate on catalytic activity, amount, and rate of de novo synthesis of hepatic histidase.
The mechanisms by which estrogen, glucocorticoid, glucagon, and adenosine 3':5'-monophosphate (cAMP), regulators which participate in the postnatal development of rat liver histidase, elevate the catalytic activity of this enzyme have been explored. A monospecific antibody against homogeneously purified preparations of rat liver histidase has been elaborated in the goat. Employing this antibody in immunotitration experiments, it has been demonstrated that the elevations of hepatic histidase activity elicited by administration in vivo of estradiol-17beta, cortisol acetate, glucagon, and N6,O2'-dibutyryl adenosine 3':5'-monophosphate (dibutyryl cAMP) are paralleled, in each instance, by equivalent increments in immunoprecipitable histidase protein. Following administration of each of the three hormones and dibutyryl cAMP, rates of [14C]leucine incorporation in vivo into rat liver histidase, isolated by immunoprecipitation, relative to incorporation rates into total soluble hepatic protein, increase in magnitudes which are comparable to increases in enzyme amount and catalytic activity. It is thus inferred that estrogen, glucocorticoids, and glucagon, via cAMP, each regulate rat liver histidase development at specific postnatal stages by inducing increases in histidase biosynthetic rates.
D000642:Ammonia-Lyases / Q000378:metabolism*; D000818:Animals; D000906:Antibodies / Q000032:analysis; D003994:Bucladesine / Q000494:pharmacology*; D002384:Catalysis; D004789:Enzyme Activation / Q000187:drug effects; D004790:Enzyme Induction / Q000187:drug effects; D004958:Estradiol / Q000494:pharmacology*; D005260:Female; D005934:Glucagon / Q000494:pharmacology*; D006638:Histidine Ammonia-Lyase / Q000096:biosynthesis / Q000276:immunology / Q000378:metabolism*; D006854:Hydrocortisone / Q000494:pharmacology*; D008099:Liver / Q000201:enzymology*; D051381:Rats
D000906:Antibodies; D004958:Estradiol; D003994:Bucladesine; D005934:Glucagon; D000642:Ammonia-Lyases; D006638:Histidine Ammonia-Lyase; D006854:Hydrocortisone