A rapid microassay for detecting antibodies against poliovirus based on [14C]thymidine uptake of treated cell cultures.
DNA synthesis of mammalian cells propagated in microplates can easily be measured if cell cultures incubated with [14C]thymidine are harvested on the glass fibre filters by a semiautomatic harvesting technique. Soon after infection with poliovirus, [14C]thymidine uptake of U cells (established, human amniotic cell line) is inhibited. This inhibition can be prevented by previous virus neutralization with antibody. Based on this effect a rapid, precise assay method was set up to determine neutralizing antibody titres against poliovirus. There was a good correlation between titres obtained by this assay and those obtained by 50% end point titrations in cytopathogenic effect inhibition assays.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002250:Carbon Radioisotopes; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D004306:Dose-Response Relationship, Immunologic; D000882:Haplorhini; D006801:Humans; D007118:Immunoassay; D017955:Poliovirus / Q000276:immunology*; D011817:Rabbits; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors
D000914:Antibodies, Viral; D002250:Carbon Radioisotopes; D013936:Thymidine