Detection and semiquantitative estimation of thyroxine and diiodothyronine in liothyronine sodium.
A method is presented for the detection and semiquantitative estimation of thyroxine and diiodothyronine in liothyronine sodium. Thyroxine is detected by thin layer chromatography, using silica gel H plates developed in butanol-acetone-ammonia (30 + 55 + 15) and sprayed with a 2,7-dichlorofluorescein solution, and estimated by comparison with standard spots. For quantitative results, a larger amount of sample is applied as a streak on a fluorescent silica gel H plate. After a 5 hr development, the band is scraped off and extracted in a small volume of 0.1N NaOH; the extract is centrifuged, transferred to a 2 cm microcell, and examined spectrophotometrically. The diiodothyronine content of liothyronine sodium is estimated by liquid chromatography on a Bondapak C18 column with 0.01N sodium perchloratebutanol-acetonitrile (1000 +62 + 188) as the eluting solvent.
D002853:Chromatography, Liquid; D002855:Chromatography, Thin Layer; D005782:Gels; D012822:Silicon Dioxide; D013970:Thyronines / Q000031:analogs & derivatives* / Q000032:analysis; D013974:Thyroxine / Q000032:analysis*; D014284:Triiodothyronine / Q000032:analysis*
D005782:Gels; D013970:Thyronines; D014284:Triiodothyronine; D012822:Silicon Dioxide; D013974:Thyroxine