Biological characterization of an Epstein-Barr nuclear antigen-positive American Burkitt's tumor-derived cell line.
Two distinct cultures derived from a lymphoid cell line designated NAB were characterized immunologically, morphologically, and cytogenetically. Both cultures were positive for Epstein-Barr nuclear antigen. NAB I cultures were negative for virus capsid antigen and early antigen and were not affected by treatment with 5-iododeoxyurdine. NAB II cultures were positive for virus capsid antigen and early antigen, which increased with 5-iododeoxyuridine treatment. Both cultures were superinfected with virus prepared from P3HR-1 cells. Cell-free virus concentrates prepared from both cultures were inactive for transformation and infectivity. NAB I and NAB II cells were lymphoid as determined by light and electron microscopy. NAB II cells showed morphological alterations characteristic of herpes infection. 5-iododeoxyuridine-treated cells from both cultures revealed ultrastructural characteristics of cells infected with herpes-viruses but without particles. In addition, the induction of tubuloreticular structures within the endoplasmic reticulum was observed. Cytogenetic analysis of both cultures revealed a rearranged chromosome 14 and several other chromosome aberrations, three of which may be used as a reliable means of identifying NAB cultures.
D000956:Antigens, Viral; D002051:Burkitt Lymphoma / Q000235:genetics / Q000276:immunology* / Q000648:ultrastructure; D002213:Capsid / Q000276:immunology; D002460:Cell Line; D002467:Cell Nucleus / Q000276:immunology; D002869:Chromosome Aberrations; D004721:Endoplasmic Reticulum / Q000187:drug effects / Q000648:ultrastructure; D004854:Herpesvirus 4, Human / Q000276:immunology*; D006801:Humans; D007065:Idoxuridine / Q000494:pharmacology; D007181:Inclusion Bodies, Viral; D014178:Translocation, Genetic
D000956:Antigens, Viral; D007065:Idoxuridine