Regulation of herpesvirus macromolecular synthesis. VI. Synthesis and modification of viral polypeptides in enucleated cells.
Cells were enucleated with cytochalasin B after infection with herpes simplex virus 1. When protein synthesis was blocked by cycloheximide from the time of infection, mRNA for viral alpha-infected cell polypeptides (ICP) 4, 0, and 27 accumulated in the cytoplasm and was expressed after the removal of both drug and nucleus. A host protein, ICP 22, whose synthesis is stimulated in intact cells, was not made, and viral protein ICP4, which is normally modified to a form that migrates more slowly in polyacrylamide gels, was not modified in the absence of the nucleus. After enucleation at 2 h postinfection, a number of viral beta and gamma proteins continued to be made, starting at 20 to 25% of the normal rates and declining with a half-time of about 2 h. The synthesis of ICP 4 declined more rapidly, suggesting that it is switched off in the cytoplasm.
D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects; D003513:Cycloheximide / Q000494:pharmacology; D003571:Cytochalasin B / Q000494:pharmacology; D003593:Cytoplasm / Q000378:metabolism; D000882:Haplorhini; D010452:Peptide Biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D012367:RNA, Viral / Q000096:biosynthesis; D018139:Simplexvirus / Q000378:metabolism*; D014764:Viral Proteins / Q000096:biosynthesis*
D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; D003571:Cytochalasin B; D003513:Cycloheximide