[Studies of spin-labeled spectrin].
Spectrin isolated from human erythrocytes has been spin-labeled with five maleimide nitroxides. The mobility of the labels is strongly dependent on their size, and on the temperature. A thermal transition of spectrin is shown to occur above 30 degrees C. Calcium and magnesium provoke a strong immobilization of the labels. This effect is yet more pronounced when spectrin is allowed to reassociate with the cytoplasmic surface of the membrane.
D004578:Electron Spin Resonance Spectroscopy; D006801:Humans; D008301:Maleimides; D008565:Membrane Proteins; D013049:Spectrin; D013113:Spin Labels; D013329:Structure-Activity Relationship; D013696:Temperature
D008301:Maleimides; D008565:Membrane Proteins; D013113:Spin Labels; D013049:Spectrin
Etude de la spectrine par marquage de spin.