Development and applications of Bacillus subtilis test systems for mutagens, involving DNA-repair deficiency and suppressible auxotrophic mutations.
A mutagen-tester of Bacillus subtilis was constructed and tested with known carcinogens. The parental strain HA101 of Okubo and Yanagida carrying suppressible nonsense mutations in his and met genes was transformed to carry an excision-repair deficiency mutation. The constructed strain TKJ5211 showed a 20--30-fold higher sensitivity for His+ reversion than the parental strain when treated with UV and UV-mimetic chemicals but unchanged mutation frequency with X-rays and methyl methanesulfonate. The tester strain was used in a spot test of 30 selected chemicals and also for testing with liver homogenate activation. The results showed an almost equivalent but somewhat broader detection spectrum than the Salmonella typhimurium TA100 system. Another test method used a pair of B. subtilis strains differing in their DNA-repair capacity, i.e. the most UV-sensitive mutant HJ-15 and a wild-type strain, to detect repair-dependent DNA damage produced by chemicals. Spores could be used in either test.
D000818:Animals; D001412:Bacillus subtilis / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D004260:DNA Repair; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D005787:Gene Frequency; D008099:Liver / Q000502:physiology; D008698:Mesylates / Q000494:pharmacology*; D008741:Methyl Methanesulfonate / Q000494:pharmacology*; D008954:Models, Biological; D009154:Mutation; D051381:Rats; D014170:Transformation, Genetic; D014466:Ultraviolet Rays
D008698:Mesylates; D008741:Methyl Methanesulfonate