title: Calcium dependency of excitatory chemical synaptic transmission in the frog cerebellum in vitro.
abstract: Chemical synaptic transmission was studied with microelectrode techniques in isolated frog cerebella maintained in vitro. Purkinje cell (PC) EPSPs, elicited by selective monosynaptic electrical stimulation of both the parallel fiber (PF) and climbing fiber (CF) inputs, could be inverted by depolarizing (outward) current injections. Evoked synaptic transmission at both junctions was reduced by lowering the extracellular concentration of calcium ions ([Ca2+]) below 2 mM. Raising [Ca2+] above 2 mM to 8 mM did not further increase synaptic transmission. Mg2+, Sr2+, and Ba2+ did not substitute for Ca2+ in the transmission process.
mesh_terms: D000818:Animals; D001464:Barium / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology / Q000502:physiology*; D002531:Cerebellum / Q000187:drug effects / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D008274:Magnesium / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects; D011689:Purkinje Cells / Q000502:physiology; D011894:Rana pipiens; D013324:Strontium / Q000494:pharmacology; D013570:Synaptic Membranes / Q000187:drug effects; D009435:Synaptic Transmission / Q000187:drug effects
chemical_list: D001464:Barium; D008274:Magnesium; D002118:Calcium; D013324:Strontium