Effect of tumor promoters on the activity of cyclic adenosine 3':5'-monophosphate-dependent and -independent protein kinases from mouse epidermis.
Cyclic adenosine 3'5'-monophosphate (cyclic AMP)-dependent and -independent protein kinases were detected and partially characterized in soluble extracts from mouse epidermis. Cylic AMP-dependent histone kinase activity was separated rom cyclic AMP-independent casein kinase activity by DEAE-Sephadex chromatography. The application of the tumor promoters croton oil or 12-o-tetradecanoyl-phorbol-13-acetate to mouse skin caused a rapid increase in the soluble protein extractable from the epidermis resulting in a decrease in the specific activity of both classes of protein kinase when expressed on a protein basis. No change in the activities of either the cyclic AMP-dependent or -independent enzymes was observed when expressed relative to the DNA content.
D000818:Animals; D002364:Caseins / Q000378:metabolism; D003436:Croton Oil / Q000494:pharmacology*; D000242:Cyclic AMP / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D006657:Histones / Q000378:metabolism; D051379:Mice; D010704:Phorbols / Q000494:pharmacology*; D011494:Protein Kinases / Q000378:metabolism*; D012867:Skin / Q000201:enzymology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*
D002364:Caseins; D006657:Histones; D010704:Phorbols; D003436:Croton Oil; D004247:DNA; D000242:Cyclic AMP; D011494:Protein Kinases; D013755:Tetradecanoylphorbol Acetate