[Role of macrophage destruction products in the alveolar phagocytosis reaction].
Peritoneal macrophages (PM) of Wistar rats harvested after the intraperitoneal injection of paraffin oil were destroyed by repeated freezing-thawing. When injected intratracheally to control rats or to those after 4 daily exposured to TiO2 dust, these macrophage destruction products (MDP) caused a significant rise of both the alveolar macrophages (AM) and the neutrophilic leukocytes (NL) counts in the pulmonary washing-outs; the mean NL/AM ratio increased several times as compared to rats injected with normal saline intratracheally. Thus, the response to the inert dust particles plus the exogenous MDP became similar to the one observed after the cytotoxic (for instance silica) particles inhalation. Enhancing the NL contribution to the inhaled particles phagocytosis, the MDP led to a significant decrease of the mean "Dust load" of a single AM, although the total number of the engulfed particles increased. The predominant attraction of granulocytes and particularly of the NL as compared to the peritoneal macrophages was also found in the peritoneal exudates of rats injected with the MDP or silica suspension intraperitoneally, while the alveolar phagocytosis was not influenced. In vitro the MDP was shown to stimulate the NL migration and to facilitate the O2 consumption by PM. A possible role of the MDP as a multipotent controlling factor of phagocytosis response is briefly discussed.
D000818:Animals; D002457:Cell Extracts / Q000494:pharmacology*; D004391:Dust; D008264:Macrophages / Q000276:immunology; D008297:Male; D009504:Neutrophils / Q000276:immunology; D010087:Oxides; D010587:Phagocytosis; D011650:Pulmonary Alveoli / Q000166:cytology*; D012822:Silicon Dioxide; D014020:Tissue Extracts / Q000494:pharmacology*; D014025:Titanium
D002457:Cell Extracts; D004391:Dust; D010087:Oxides; D014020:Tissue Extracts; D012822:Silicon Dioxide; D014025:Titanium
O roli produktov razrusheniia makrofaga v reaktsii al'veoliarnogo fagotsitoza