A sensitive method for the detection of herpes simplex virus type 2 specific thymidine kinase.
Thymidine kinase (TK) activity of uninfected and in vitro herpes simplex virus type 2 (HSV-2) infected human fetal lung cells was analyzed. Polyacrylamide gel electrophoresis was carried out, and subsequent incubation of equally divided gel slices in the presence of normal or anti-HSV-2 specific TK serum, together with different substrates, was performed. Small amounts of virus-specific enzyme could be detected using this combination of electrophoresis and antibodies and replacing ATP by CTP in the substrate medium.
D000255:Adenosine Triphosphate / Q000378:metabolism; D046508:Culture Techniques; D003597:Cytosine Nucleotides / Q000378:metabolism; D003600:Cytosol / Q000201:enzymology; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007106:Immune Sera; D008928:Mitochondria / Q000201:enzymology; D018139:Simplexvirus / Q000201:enzymology*; D013937:Thymidine Kinase / Q000032:analysis* / Q000276:immunology / Q000378:metabolism
D003597:Cytosine Nucleotides; D007106:Immune Sera; D000255:Adenosine Triphosphate; D013937:Thymidine Kinase