Characteristics of a human cell line successively transformed by Rous sarcoma virus and simian virus 40.
Human enbryo cells were successively transformed by the Schmidt-Ruppin strain of Rous sarcoma virus (SR-RSV) and simian virus 40 (SV40) in vitro, and the double transformant HuE 13 RS was established. From this cell line the two clonal cell lines RSa and RSb were isolated. In both, presence of SV40 T antigens was demonstrated by the fluorescent antibody technique, and the presence of RSV genomes was verified in one RSb clone by focus formation after fusion with chick embryo cells. Growth of these cells was affected by dibutyryl cAMP without marked morphologic changes. Cells were extremely sensitive to the anticellular action of human leukocyte interferon.
D000956:Antigens, Viral; D001358:Avian Sarcoma Viruses; D003994:Bucladesine / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D004622:Embryo, Mammalian; D005796:Genes; D006367:HeLa Cells / Q000187:drug effects; D006801:Humans; D007372:Interferons / Q000494:pharmacology; D009368:Neoplasm Transplantation; D013539:Simian virus 40 / Q000276:immunology; D010948:Viral Plaque Assay
D000956:Antigens, Viral; D003994:Bucladesine; D007372:Interferons