Effect of rhesus or vervet cytomegalovirus infection of DNA synthesis in untreated and 5-iodo-2'-deoxyuridine-arrested cells.
Infection of permissive cells with either rhesus or vervet cytomegalovirus resulted in suppression of cellular DNA synthesis, only viral DNA was resolved by isopycnic centrifugation after 24 h postinoculation. Infection of 5-iodo-2'-deoxyuridine (IUdR)-arrested cells with either of the simian cytomegaloviruses, however, induced synthesis of cellular DNA of normal density; synthesis of cellular DNA substituted with IUdR as evidenced by resolution of a heavy DNA peak after isopycnic centrifugation was not observed. Stimulation of DNA synthesis in IUdR-arrested cells was not observed with virus inactivated with UV light.
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D003587:Cytomegalovirus / Q000254:growth & development* / Q000378:metabolism / Q000528:radiation effects; D004247:DNA / Q000096:biosynthesis*; D004279:DNA, Viral / Q000096:biosynthesis; D000882:Haplorhini; D006801:Humans; D007065:Idoxuridine / Q000494:pharmacology*; D008253:Macaca mulatta; D014466:Ultraviolet Rays; D014779:Virus Replication
D004279:DNA, Viral; D004247:DNA; D007065:Idoxuridine