title: Effect of carbon source on enzymes involved in glycerol metabolism in Neurospora crassa.
abstract: Specific activities of eight enzymes involved in glycerol metabolism were determined in crude extracts of three strains of Neurospora crassa after growth on six different carbon sources. One of the strains was wild type, which grew poorly on glycerol as sole carbon source; the other two were mutant strains which were efficient glycerol utilizers. A possible basis for this greater efficiency of glycerol utilization was catabolite repression of glyceraldehyde kinase by glycerol in wild type, and two-fold higher glycerate kinase activity in the mutant strains after growth on glycerol, thus apparently allowing two routes for glyceraldehyde to enter the glycolytic pathway in the mutant strains but only one in wild type. The preferential entry of glyceraldehyde to the glycolytic pathway through glycerate was suggested by the lack of glyceraldehyde kinase in all three strains after growth on one or more of the carbon sources and the generally higher levels of aldehyde dehydrogenase and of glycerate kinase than of glyceraldehyde kinase.
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000378:metabolism; D002244:Carbon / Q000378:metabolism*; D002474:Cell-Free System; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism*; D005991:Glycerol Kinase / Q000378:metabolism; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D009491:Neurospora / Q000201:enzymology*; D009492:Neurospora crassa / Q000201:enzymology* / Q000378:metabolism; D010770:Phosphotransferases / Q000378:metabolism; D013401:Sugar Alcohol Dehydrogenases / Q000378:metabolism; D014305:Triose-Phosphate Isomerase / Q000378:metabolism
chemical_list: D002244:Carbon; D005993:Glycerolphosphate Dehydrogenase; D013401:Sugar Alcohol Dehydrogenases; D000445:Aldehyde Oxidoreductases; D010770:Phosphotransferases; D005991:Glycerol Kinase; D005634:Fructose-Bisphosphate Aldolase; D014305:Triose-Phosphate Isomerase; D005990:Glycerol