title: [125I] Glucagon binding by liver membranes from young and adult rats.
abstract: We have previously shown that adipocytes from adult (between four and five months old) rats have reduced glucagon binding and glucagon-stimulated lipolytic activity when compared with cells from young (1.5 months old) animals. In the present study we measured specific [125I] glucagon binding by purified liver plasma membranes isolated from young and adult rats. When expressed on the basis of membrane protein content, 5'-nucleotidse activity, or specific [125I] insulin binding, the extend of [125I] glucagon binding by liver membranes was not influenced by aging. Furthermore, the degree of [125I] glucagon degradation was the same in both membrane preparations. These data describe a unique condition in which glucagon binding and hormone sensitivity diminish in one tissue but remain unaltered in another.
mesh_terms: D000375:Aging; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D005934:Glucagon / Q000378:metabolism*; D007328:Insulin / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000254:growth & development / Q000378:metabolism*; D051381:Rats; D011972:Receptor, Insulin / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism
chemical_list: D007328:Insulin; D011956:Receptors, Cell Surface; D005934:Glucagon; D011972:Receptor, Insulin