[Isolation of various structural elements of gram-negative bacteria of the Bordetella genus and a study of their properties. II. Isolation of the cytoplasmic membrane fraction from the protoplasts of B. pertussis and a study of their chemical and enzymatic properties].
A possibility of obtaining a fraction of cytoplasmic membranes maximally purified of the cell wall material was for the first time shown on a model of Gram negative bacteria (B. pertussis). The results of electron microscopy, chemical analysis for the presence of the cell wall material--hexosamines, and of the activity of the respiratory chain enzymes served as control.
D001886:Bordetella pertussis / Q000648:ultrastructure*; D002458:Cell Fractionation; D002462:Cell Membrane / Q000032:analysis / Q000201:enzymology / Q000648:ultrastructure; D006595:Hexosamines / Q000032:analysis; D011523:Protoplasts / Q000648:ultrastructure
D006595:Hexosamines
Vydelenie nekotorykh strukturnykh élementov gramotritsatel'nykh bakteriĭ roda Bordetella i izuchenie ikh svoĭstv. Soobshchenie II. Vydelenie fraktsii tsitoplazmaticheskikh membran iz protoplastov B. pertussis i izuchenie ikh khimicheskikh i nekotorykh énzimaticheskikh svoĭstv