title: Cell-free synthesis of herpes simplex virus proteins.
abstract: Polyribosomes isolated from herpes simplex virus type I (HSV-1)-infected cells have been used to program a eucaryotic cell-free translation system. At least 10 HSV-specific polypeptides, with apparent molecular weights of 25,000 to 160,000, are synthesized by wild-type HSV-infected polyribosomes. Polyribosomes prepared from thymidine kinase-negative mutants of HSV direct the synthesis of three putative nonsense termination polypeptides. HSV-specific polypeptides synthesized in vitro are precipitated with antiserum to HSV-infected cell proteins.
mesh_terms: D000818:Animals; D002460:Cell Line; D002474:Cell-Free System; D000882:Haplorhini; D008274:Magnesium / Q000494:pharmacology; D008970:Molecular Weight; D010452:Peptide Biosynthesis; D011132:Polyribosomes / Q000378:metabolism; D011700:Putrescine / Q000378:metabolism; D018139:Simplexvirus / Q000276:immunology / Q000378:metabolism*; D013937:Thymidine Kinase / Q000378:metabolism; D014764:Viral Proteins / Q000096:biosynthesis* / Q000276:immunology
chemical_list: D014764:Viral Proteins; D013937:Thymidine Kinase; D008274:Magnesium; D011700:Putrescine