Determination of the solution conformation of adenosein 2':3'-monophosphate by nuclear magnetic resonance with lanthanide probes.
The aqueous solution conformation of adenosine 2':3'-monophosphate at pH 2.5 has been determined by a nuclear magnetic resonance method utilizing lanthanide ions as shift and relaxation probes. The ribose conformation is best described as a rapid equilibrium of 2'-endo(3'-exo) and 3'-endo(2'-exo) conformations in a ratio of approximately 2 to 1. The orientation of the base relative to ribose is restricted to a narrow range about chiCN=-70 degrees.
D000242:Cyclic AMP; D007536:Isomerism; D009682:Magnetic Resonance Spectroscopy; D008674:Metals, Rare Earth; D008968:Molecular Conformation
D008674:Metals, Rare Earth; D000242:Cyclic AMP