[Participation of cholinergic mechanisms in realizing the effects of bicuculline].
Screening and electrophysiological methods were applied to the verification of the hypothesis on a possibility of participation of cholinergic structures in the realization of bicucullin effects. M- and N-cholinolytics (benactizine, atropine, aprophen, and pediphen) failed to arrest the convulsions induced in mice by bicucullin adminstration. At the same time substances inducing accumulation of gamma-aminobutyric acid (GABA) in the brain, i.e. aminooxycetic acid and depakin produced a manifest protective action in convulsions caused by bicucullin administration. In electrophysiological experiments there was also revealed an incapacity of M-cholinolytic benaltizine to arrest the bicucullin effects. Bicucullin proved to diminish depression of the test response in the restoration cycle of the primary response of the rat sensory motor cortex at the intervals of 40--125ms between the stimuli, whereas benactizine decreased the late facilitation of the test response at the intervals of 150--300ms between the stimuli. There was also noted no interaction between benactizine and bicucullin by this test. On the basis of these data a conclusion was drawn that bicucullin effects were caused by the block of postsynaptic GABA receptors, and were not connected with the cholinergic structures activity.
D000818:Animals; D001535:Benactyzine / Q000494:pharmacology; D001640:Bicuculline / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D002540:Cerebral Cortex / Q000187:drug effects*; D018756:GABA Antagonists; D007546:Isoquinolines / Q000494:pharmacology*; D051379:Mice; D010276:Parasympatholytics / Q000494:pharmacology; D051381:Rats; D017981:Receptors, Neurotransmitter / Q000187:drug effects*; D012640:Seizures / Q000139:chemically induced
D018756:GABA Antagonists; D007546:Isoquinolines; D010276:Parasympatholytics; D017981:Receptors, Neurotransmitter; D001535:Benactyzine; D001640:Bicuculline
K voprosu ob uchastii kholinergicheskikh mekhanizmov v realizatsii effektov bikukullina.