Cell shape and hexose transport in normal and virus-transformed cells in culture.
The rate of hexose transport was compared in normal and virus-transformed cells on a monolayer and in suspension. It was shown that: 1) Both trypsin-removed cells and those suspended for an additional day in methyl cellulose had decreased rates of transport and lower available water space when compared with cells on a monolayer. Thus, cell shape affects the overall rate of hexose transport, especially at higher sugar concentrations. 2) Even in suspension, the initial transport rates remained higher in transformed cells with reference to normal cells. Scanning electron micrographs of normal and transformed chick cells revealed morphological differences only in the flat state. This indicates that the increased rate of hexose transport after transformation is not due to a difference in the shape of these cells on a monolayer.
D000818:Animals; D001358:Avian Sarcoma Viruses; D001692:Biological Transport; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D002642:Chick Embryo; D003847:Deoxyglucose / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D005947:Glucose / Q000378:metabolism*; D008747:Methylcellulose; D008757:Methylglucosides / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D013936:Thymidine / Q000378:metabolism; D013997:Time Factors; D014357:Trypsin / Q000494:pharmacology
D008757:Methylglucosides; D008747:Methylcellulose; D003847:Deoxyglucose; D014357:Trypsin; D005947:Glucose; D013936:Thymidine