title: Transport as a rate limiting step in glucose metabolism in virus-transformed cells: studies with cytochalasin B.
abstract: Steady-state kinetic tracer analysis and two-dimensional chromatography and autoradiography were used to examine the relation of glucose transport to its metabolism in cultured chick embryo fibroblasts. Cytochalasin B was added to Rous sarcoma virus-infected cells to bring the rate of glucose uptake to the level of uninfected cells. It is concluded for chick cells in culture that: (1) the transport of glucose, rather than its phosphorylation, is the rate limiting step and (2) the difference in aerobic glycolysis between normal and virus-transformed cells at physiological glucose concentration may be a consequence  of the difference in the rates of glucose uptake.
mesh_terms: D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000332:Aerobiosis; D000818:Animals; D001358:Avian Sarcoma Viruses; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D002642:Chick Embryo; D002952:Citric Acid Cycle; D003571:Cytochalasin B / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism*; D006003:Glycogen / Q000096:biosynthesis; D006019:Glycolysis; D007773:Lactates / Q000096:biosynthesis; D010428:Pentosephosphates / Q000378:metabolism
chemical_list: D007773:Lactates; D010428:Pentosephosphates; D003571:Cytochalasin B; D000255:Adenosine Triphosphate; D006003:Glycogen; D005947:Glucose