Characterization of a feline sarcoma virus-coded antigen (FOCMA-S) by radioimmunoassay.
A radioimmunoassay has been developed that detects a unique antigen encoded by the genome of the feline sarcoma virus (FeSV). Pseudotype viral particles containing an FeSV-specific polyprotein (p85) were used both as a source of antigen and to prepare specific antisera in rabbits. Because p85 contains antigens related to two structural proteins (p15 and p12) of feline leukemia virus (FeLV), antibodies directed to these were adsorbed with purified FeLV proteins. The adsorbed rabbit antiserum bound to antigenic determinants (designated FOCMA-S) which are also present in p85 and reacted specifically in immunofluorescence tests with rat cells transformed by FeSV and with FOCMA-positive cat lymphoid tumor cells. Competition assays detect FOCMA-S in pseudotype type C viruses rescued from FeSV-transformed mink and rat cells but not in heterologous type C helper viruses or in FeLV. A crossreactive antigen was also detected in pseudotypes of Kirsten sarcoma virus. The assay permits the quantitative measurement of an FeSV-coded protein whose expression is associated with viral transformation.
D000918:Antibody Specificity; D000956:Antigens, Viral / Q000032:analysis*; D002460:Cell Line; D002472:Cell Transformation, Viral; D005256:Leukemia Virus, Feline / Q000032:analysis / Q000276:immunology; D008970:Molecular Weight; D011863:Radioimmunoassay / Q000379:methods; D012190:Retroviridae / Q000276:immunology*; D005253:Sarcoma Viruses, Feline / Q000276:immunology*; D014764:Viral Proteins / Q000032:analysis
D000956:Antigens, Viral; D014764:Viral Proteins