Extraneuronal effects of 6-hydroxydopamine and extraneuronal uptake of noradrenaline. In-vivo and in-vitro studies on adrenocortical cells of lizards and rats.
6-hydroxydopamine (6-OHDA) was shown to cause ultrastructural changes in adrenocortical cells of lizards and rats. These changes comprised the formation of dense bodies with lamellar and crystalloid patterns, a decrease in the number of mitochondria and structural alterations of mitochondria. Alterations in adrenomedullary cells were not affected as a rule. Only in young animals did 6-OHDA cause deposits of an electron-dense material in medullary cells. An attempt was made to obtain information on amine uptake into cortical cells using the Falck-Hillarp technique to analyse the in-vivo and in-vitro uptake of noradrenaline (NA) into the adrenal cortex in adult rats. Extraneuronal uptake into heart and spleen was studied as well. Our results suggest that NA is taken up into cortical cells, particularly into nuclei, after exposure to 10(-4) gm/ml in-vitro indicating that uptake of 6-OHDA is also likely. Investigations using labelled 6-OHDA is also likely. Investigations using labelled 6-OHDA are required for further elucidating its extraneuronal uptake.
D000302:Adrenal Cortex / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D000311:Adrenal Glands / Q000187:drug effects*; D000818:Animals; D005260:Female; D006892:Hydroxydopamines / Q000378:metabolism / Q000494:pharmacology*; D002479:Inclusion Bodies / Q000648:ultrastructure; D008116:Lizards; D008297:Male; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000648:ultrastructure; D009206:Myocardium / Q000378:metabolism; D009638:Norepinephrine / Q000378:metabolism*; D051381:Rats; D013154:Spleen / Q000378:metabolism
D006892:Hydroxydopamines; D009638:Norepinephrine