Electron paramagnetic resonance of Hb St Louis beta28 (B10) Leu replaced by Gln.
Hemoglobin St Louis beta28 (B10) Leu replaced by Gln is a new mutant which occurs as a natural valency hybrid (alpha2beta+2), or hemoglobin M (Cohen-Solal, M., Seligmann, M., Thillet, J. and Rosa, J. (1973) FEBS Lett. 33, 37-41). The electron paramagnetic resonance (EPR) spectrum of native Hb St Louis at pH 6.2 shows a mixture of three species. Two are high spin, one with tetragonal symmetry, like Hb+ A, the other with rhombic distortion. The third is a low-spin form corresponding to a hemichrome with the distal (E7) histidine as the sixth ligand of the ferric iron. The hemichrome is also found in red blood cells. After oxidation to the alpha+2beta+2 form, three EPR species are seen. Surprisingly, there remains only one high-spin signal, with almost tetragonal symmetry. Besides the low-spin hemichrome, a hydroxy signal is observed even at pH 6.2. These observations imply interactions between the alpha and beta hemes.
D004578:Electron Spin Resonance Spectroscopy; D005973:Glutamine; D006441:Hemoglobin A; D006449:Hemoglobin M; D006455:Hemoglobins, Abnormal; D006801:Humans; D007930:Leucine; D011487:Protein Conformation
D006455:Hemoglobins, Abnormal; D005973:Glutamine; D006441:Hemoglobin A; D006449:Hemoglobin M; D007930:Leucine