Separation of oligo(adenosine diphosphate ribose) fractions with various chain lengths and terminal structures.
Oligo(adenosine diphosphate ribose) preparations with chain lengths of 3 to 10 adenosine diphosphate ribose units were fractionated according to their chain lengths and their terminal structures by hydroxyapatite column chromatography and then polyacrylamide gel electrophoresis. The peak fractions from the hydroxyapatite column were each separated into two distinct subfractions by gel electrophoresis. The two subfractions were found to differ in chain length and terminal structure. A linear correlation was observed between the mobility and the logarithm of the chain length of oligo(adenosine diphosphate ribose) on gel electrophoresis, irrespective of the terminal structure.
D000469:Alkaline Phosphatase; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008970:Molecular Weight; D009702:Nucleoside Diphosphate Sugars / Q000096:biosynthesis*; D009841:Oligonucleotides / Q000096:biosynthesis; D009843:Oligoribonucleotides / Q000096:biosynthesis / Q000302:isolation & purification; D010744:Phosphoric Monoester Hydrolases; D011064:Poly Adenosine Diphosphate Ribose / Q000096:biosynthesis*; D051381:Rats
D009702:Nucleoside Diphosphate Sugars; D009841:Oligonucleotides; D009843:Oligoribonucleotides; D011064:Poly Adenosine Diphosphate Ribose; D000469:Alkaline Phosphatase; D010744:Phosphoric Monoester Hydrolases