Immunoelectrophoretic characterization of Sendai virus antigens.
Antigens extracted from Sendai virus by solubilization with Triton X-100 (Tx). Tween-20 (Tw) or Triton X-100 and SDS (Tx-SDS) were analysed by rocket, crossed and tandem immunoelectrophoresis. The migration of these antigens through 1% agarose supplemented with guinea pig anti-viral envelope protein anti-serum shows that Tx and Tw antigens consist of three fractions differing in their quantitative ratios, while Tx-SDS exhibits five different fractions. The method proved to be useful for the identification of anti-host antibodies in immune antisera by means of an antigen extracted from the chorio-allantoic membrane of embryonated hen eggs.
D000956:Antigens, Viral / Q000032:analysis* / Q000302:isolation & purification; D007122:Immunoelectrophoresis; D010222:Parainfluenza Virus 1, Human / Q000032:analysis*
D000956:Antigens, Viral