New test for endotoxin potency based upon histamine sensitization in mice.
The results of a test of endotoxic potency based upon the development of histamine hypersensitivity in mice were compared with the results obtained by testing the same materials for pyrogenicity in rabbits and lethality for chicken embryos (CELD50). The results of the histamine hypersensitization test (HHT) correlated well with those of the other two tests. The sensitivity of the HHT was about the same as that of the CELD50 assay. The HHT may provide a relatively inexpensive, fast, and reliable assay method for endotoxin laboratories that do not have the facilities for the more elaborate assays.
D000818:Animals; D001681:Biological Assay / Q000379:methods*; D001886:Bordetella pertussis; D002003:Brucella abortus; D004731:Endotoxins / Q000032:analysis* / Q000494:pharmacology; D004926:Escherichia coli; D006632:Histamine; D006969:Hypersensitivity, Immediate / Q000209:etiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D010944:Plants; D012475:Salmonella
D004731:Endotoxins; D006632:Histamine