The mechanism of actions of collagenase on the inhibition of release of acetylcholine from synaptosomal preparations.
The release of acetylcholine from synaptosomal preparations from bovine superior cervical ganglia and rat cortex was inhibited when the preparations were pretreated with collagenase. The inhibition of release could be overcome with the calcium ionophore A23187. Collagenase treatment was shown to inhibit the uptake of calcium into the preparations. In addition, gel electrophoresis of synaptosomal membranes revealed two missing high molecular weight proteins when either synaptosomes or synaptosomal membranes were incubated with collagenase.
D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002417:Cattle; D002540:Cerebral Cortex / Q000187:drug effects; D005725:Ganglia, Autonomic / Q000187:drug effects; D066298:In Vitro Techniques; D003012:Microbial Collagenase / Q000494:pharmacology*; D009419:Nerve Tissue Proteins / Q000378:metabolism; D051381:Rats; D013574:Synaptosomes / Q000187:drug effects* / Q000648:ultrastructure
D009419:Nerve Tissue Proteins; D000001:Calcimycin; D003012:Microbial Collagenase; D000109:Acetylcholine; D002118:Calcium