A specific method for the determination of threonine in rat blood plasma using aldehyde dehydrogenase.
A simple and sensitive method for the determination of threonine in rat blood plasma using aldehyde dehydrogenase after oxidation with periodate was developed. By the present method, threonine could be completely discriminated from serine and determined at the nanomole level. The amount of threonine in rat blood plasma obtained by the present method coincided well with the value determined on an amino acid analyzer.
D000445:Aldehyde Oxidoreductases / Q000378:metabolism*; D000818:Animals; D005947:Glucose; D010504:Periodic Acid; D051381:Rats; D012694:Serine; D013050:Spectrometry, Fluorescence; D013912:Threonine / Q000097:blood*
D010504:Periodic Acid; D013912:Threonine; D012694:Serine; D000445:Aldehyde Oxidoreductases; D005947:Glucose