Cyclic AMP modulation of calcium accumulation by sarcoplasmic reticulum from fast skeletal muscle.
The role of cyclic 3',5'-AMP in modulating sarcoplasmic reticulum from fast skeletal muscle was studied. The rate of Ca2+ uptake was stimulated in the presence of protein kinase plus 1 micron cyclic AMP. The stimulation was absent when denatured protein kinase was used. When an adenylate cyclase inhibitor was added, the uptake rates fell to 55% of control. This decrease in rate was partially overcome by 1 micron cyclic AMP. A modulating role for cyclic AMP in fast skeletal muscle is proposed.
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000067956:Adenylyl Cyclase Inhibitors; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D003470:Culture Media; D000242:Cyclic AMP / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006358:Hot Temperature; D066298:In Vitro Techniques; D009114:Murexide; D009132:Muscles / Q000378:metabolism; D011489:Protein Denaturation; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases / Q000494:pharmacology; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000201:enzymology / Q000378:metabolism*
D000067956:Adenylyl Cyclase Inhibitors; D003470:Culture Media; D004791:Enzyme Inhibitors; D047428:Protein Kinase Inhibitors; D009114:Murexide; D000242:Cyclic AMP; D011494:Protein Kinases; D000251:Adenosine Triphosphatases; D002118:Calcium