Studies on the mechanism of action of ACTH on triglyceride synthesis in fat cells.
It was found that ACTH greatly reduced lipogenesis in fat cells in the presence of calcium ion, but not in the absence of calcium ion. Of the enzymes involved in triglyceride synthesis from fatty acid in lipid micelle membranes, only acyl-CoA synthetase was inhibited by calcium ion, the apparent Ki value of calcium ion being 4.2 X 10(-4) M. The Km values of the enzyme for palmitate and ATP were 2.0 X 10(-4) M and 2.5 X 10(-4) M, respectively and calcium ion caused non-competitive inhibition with both palmitate and ATP. The acyl-CoA synthetase activity of lipid micelle membranes was inhibited by treatment with phospholipase A or C, but not by treatment with phospholipase D. The mechanism of inhibition of triglyceride synthesis by ACTH is discussed on the basis of these results.
D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D003066:Coenzyme A Ligases / Q000378:metabolism; D007700:Kinetics; D008297:Male; D051381:Rats; D014280:Triglycerides / Q000096:biosynthesis*
D014280:Triglycerides; D000324:Adrenocorticotropic Hormone; D003066:Coenzyme A Ligases; D002118:Calcium