Epidermal growth factor induced membrane changes in 3T3 cells.
Epidermal growth factor (EGF) is a mitogen for Swiss 3T3 cells. Short incubation periods with physiological concentrations of EGF induced increased binding of Swiss 3T3 cells to Con A-coated nylon fibers. This effect was not induced in an EGF non-responsive 33 variant, in the transformed murine XC cells or in Swiss SV3T3 cells. The increase in Con A fiber-binding seems to be specific for EGF, since it was not observed in response to insulin, prostaglandin F2alpha or a higher serum concentration, which also initiate cell devision of confluent quiescent 3T3 cells. EGF also reduced Con A-mediated hemadsorption to 3T3, but had no effect on hemadsorption by the EFG non-responsive 3T3 variant. There was no change in the number of Con A-receptors on 3T3 cells after EGF treatment. Binding to WGA-coated fibers and WGA-mediated hemadsorption were not effected by preincubation with EGF.
D002999:Clone Cells; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D037102:Lectins; D008934:Mitogens; D010455:Peptides / Q000494:pharmacology*; D037121:Plant Lectins; D011952:Receptors, Concanavalin A / Q000187:drug effects; D011955:Receptors, Drug / Q000187:drug effects*; D013539:Simian virus 40; D013997:Time Factors; D014908:Triticum
D037102:Lectins; D008934:Mitogens; D010455:Peptides; D037121:Plant Lectins; D011952:Receptors, Concanavalin A; D011955:Receptors, Drug; D004815:Epidermal Growth Factor