[Production and characteristics of a Djzungarian hamster cell line (DX-TK-) resistant to 5-bromodeoxyuridine].
New biochemically marked Djungarian hamster cell line (DX-TK-) was established. These cells are resistant to 5-bromodeoxyuridine (25 mkg/ml) and deficient in thymidine kinase activity (TK-). Due to this biochemical defect they have lost the ability to grow in HAT medium. DX-TK- cells are malignant. They grow as tumours after the inoculation to newborn Djungarian hamsters. Tumorigenecity of DX-TK- cells was decreased as compared with the parent TK+ cell line. DX-TK- cell line is a hypodiploid cell culture (26 chromosomes) with 7 chromosome markers easily identified by means of G-band staining. This line is a new model for somatic cell genetic experiments, particularly for somatic cell hybridization.
D000818:Animals; D001973:Bromodeoxyuridine / Q000037:antagonists & inhibitors*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D002478:Cells, Cultured / Q000187:drug effects*; D006224:Cricetinae; D004351:Drug Resistance; D066298:In Vitro Techniques; D007621:Karyotyping; D011003:Ploidies / Q000187:drug effects; D013539:Simian virus 40 / Q000472:pathogenicity
D001973:Bromodeoxyuridine
Poluchenie i kharakteristika linii kletok dzhungarskogo khomiachka (DKh-TK-), rezistentnoĭ k 5-bromdezoksiuridinu.