Growth characteristics and tumorigenicity of long-term cultures of the rat RBA myelogenous leukemia.
A continuous cell line, RBA-BC, has been derived from the buffy coat of heparinized blood of a Sprague--Dawley rat with developed RBA myeloblastosis. Separations of myeloblasts from the RBA-BC cell line was accomplished by velocity sedimentation in Verografin density gradients. The cells were monitored during long-term culture by generation time analysis. Cytochemical studies revealed the myelogenous type of RBA leukemia.
D000818:Animals; D001853:Bone Marrow; D001854:Bone Marrow Cells; D002455:Cell Division; D002460:Cell Line; D002469:Cell Separation; D007621:Karyotyping; D007942:Leukemia, Experimental / Q000209:etiology; D007951:Leukemia, Myeloid; D010503:Periodic Acid-Schiff Reaction; D009195:Peroxidase / Q000032:analysis; D051381:Rats
D009195:Peroxidase