Organ culture of adult mouse intestine IV. Stimulation of glucose-6-phosphatase in vitro.
Explants of adult mouse jejunum have been maintained in organ culture with or without fructose added to the medium in order to stimulate the intestinal glucose-6-phosphatase (G-6-Pase). When the fructose is added, at the beginning of the culture, a three-fold increase of G-6-Pase in measured during the first 24 h. If the fructose is added after 24 h of culture, no significant increase of the G-6-Pase is registered in comparison with the controls. Proteins, DNA content and dissacharidase activities are not modified during the culture. Alkaline phosphatase activity presents a twofold increase in the controls and stimulated explants. The ultrastructural localization of the G-6-Pase is not altered during the culture.
D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D005632:Fructose / Q000494:pharmacology; D005952:Glucose-6-Phosphatase / Q000032:analysis*; D006651:Histocytochemistry; D007583:Jejunum / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009924:Organ Culture Techniques
D005632:Fructose; D000469:Alkaline Phosphatase; D005952:Glucose-6-Phosphatase