Factors involved in the expression of cowpox virus-specific antigen in Sendai virus carrier cells.
Formation of cowpox virus-specific cell surface antigen (CPVS-ag) HVJ (Sendai virus) carrier cells compared to that in parent cells. Temperature shifts from 32 to 35 to 37 degrees C for these carrier cultures reduced this enhancing activity, making them equivalent to parent cells. The eclips phase and one-step growth of CPV in the carrier cells were shorter than in normal cells. Extracts of carrier cells exhibited a stimulating activity causing the temporary rise and subsequent lowering of CPV infectivity in their reactions with CPV in vitro, suggesting a cellular acceleration of CPV uncoating. The S-ag forming ability of these carrier cells did not decrease as much as that of parent cellsin the presence of actinomycin D or puromycin. The results indicate that persistent infection with HVJ, especially the temperature-sensitive variant, promotes the first step of intracellular growth of CPV, resulting in enhanced formation of S-ag.
D000818:Animals; D000956:Antigens, Viral; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D006224:Cricetinae; D003609:Dactinomycin / Q000494:pharmacology; D014644:Genetic Variation; D010222:Parainfluenza Virus 1, Human / Q000254:growth & development*; D011691:Puromycin / Q000494:pharmacology; D013696:Temperature; D014616:Vaccinia virus / Q000254:growth & development / Q000276:immunology*; D014779:Virus Replication
D000956:Antigens, Viral; D003609:Dactinomycin; D011691:Puromycin