Identification and isolation of the yeast cytochrome c gene.
The iso-1-cytochrome c gene of yeast has been identified and cloned using a synthetic oligodeoxynucleotide as a hybridization probe. The oligomer d[pT-T-A-G-C-A-G-A-A--C-C-G-G] is complementary to a region near the N terminal coding region of the yeast cyc 1 gene. Of several yeast Eco RI fragments which hybridize to this probe, one is changed in size by a G leads to T mutation which eliminates an Eco RI site within the cyc 1 gene. Both the wild-type and the RI- mutant forms were cloned in lambda gt vectors. Maxam-Gilbert sequencing for 91 nucleotides into the coding region for iso-1-cytochrome c yielded a DNA sequence in perfect correspondence with the known protein sequence.
D001483:Base Sequence; D003062:Codon; D003574:Cytochrome c Group / Q000235:genetics*; D004274:DNA, Recombinant; D005796:Genes; D010957:Plasmids; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D003062:Codon; D003574:Cytochrome c Group; D004274:DNA, Recombinant