title: Isolation and purification of lithocholic acid metabolites produced by the intestinal microflora.
abstract: Lithocholic acid metabolites produced by the intestinal microflora of rats can be isolated from other endogenous lipids using Sephadex LH-20 column chromatography. Analyses of individual metabolites collected from the column by silica gel coated glass fiber paper chromatography result in the resolution of epimeric 3-hydroxy derivatives. In addition, glass fiber paper chromatography is more sensitive and requires less development time than conventional glass-coated thin-layer plates. Further confirmation of the identity of metabolites is achieved by gas-liquid chromatography, which separates both methyl and ethyl esters of lithocholic and isolithocholic acids.
mesh_terms: D000818:Animals; D002793:Cholic Acids / Q000302:isolation & purification*; D002849:Chromatography, Gas; D002854:Chromatography, Paper; D002855:Chromatography, Thin Layer; D004952:Esters / Q000302:isolation & purification; D005782:Gels; D007422:Intestines / Q000382:microbiology*; D008095:Lithocholic Acid / Q000302:isolation & purification* / Q000378:metabolism; D008722:Methods; D051381:Rats; D012822:Silicon Dioxide
chemical_list: D002793:Cholic Acids; D004952:Esters; D005782:Gels; D008095:Lithocholic Acid; D012822:Silicon Dioxide