An evaluation of the absorption spectra of antigens from Entamoeba histolytica (Ibadan strains).
By means of chromatography different fractions of antigens from Entamoeba histolytica (Ibadan Strains) have been separated. All the different fractions have been tested for antigenic reactivity using agar gel diffusion technique. The intact antigen, as well as, the three antigenically reactive fractions were found to have ultra-violet absorption spectra in the region 258 and 278 millimicron. By virtue of this physico-chemical property amoebic extract(s) was found to simulate a mononucleotide Deoxy-5-methyl-cytidilic-acid. Such a unique structure of the extracts was used to suggest a mechanism for the possible low antigenicity of the organism based on its inability to induce a protective antibody response in human amoebiasis.
D000941:Antigens / Q000032:analysis*; D004748:Entamoeba histolytica / Q000276:immunology*; D013056:Spectrophotometry, Ultraviolet
D000941:Antigens