Thermosensitive respiratory deficiency in yeast associated with specific effects on particulate cytochrome oxidase.
A mutant of Saccharomyces cerevisiae, unable to grow at the expense of non fermentable carbon sources at 37 degrees C, has been selected; at 25 degrees C the mutant strain behaves like the parental wild strain. Evaluations of respiration rates during aerobic growth at restrictive temperature on one hand, enzymatic and/or spectral evaluations of the individual components of the respiratory chain on the other hand show that the respiratory deficiency is specifically correlated with a reduced level of cytochrome oxidase. The decrease of enzyme activity is the direct consequence of a lowering of hemoprotein (a,a3) concentration. Temperature-activity relationship of cytochrome oxidase elaborated at the permissive temperature by the mutant strain is modified as far as the particulate enzyme is concerned, but no difference is observed after partial solubilization of the enzyme by non ionic surfactant. Genetic analysis shows that the mutant phenotype results from a nuclear gene mutation.
D000332:Aerobiosis; D002455:Cell Division; D002462:Cell Membrane / Q000201:enzymology; D003576:Electron Transport Complex IV / Q000302:isolation & purification / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D006358:Hot Temperature; D007700:Kinetics; D007773:Lactates / Q000378:metabolism; D009154:Mutation; D010101:Oxygen Consumption; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D013172:Spores, Fungal / Q000378:metabolism
D007773:Lactates; D000431:Ethanol; D003576:Electron Transport Complex IV; D005947:Glucose; D005990:Glycerol