Inhibition of maturation and metabolism in rat oocytes by cyclic amp.
It is known that dibutyryl cyclic AMP (dbcAMP) and theophylline inhibit the spontaneous maturation of isolated mouse oocytes. The present study demonstrates that dbcAMP (0.01-1.0 mM) as well as cyclic AMP (cAMP, 10 mM) and a phosphodiesterase inhibitor (IBMX, 0.01-1.0 mM) prevent spontaneous maturation of isolated rat oocytes. As reported earlier an increase in oxygen consumption by the oocyte was found following maturation. When the oocytes were cultured in the presence of dbcAMP or cAMP no change in respiration occurred during culture. These results argue against the theory that cAMP acts as a direct mediator of the action of luteinizing hormone (LH) on oocyte maturation. Furthermore they suggest that changes in oocyte energy metabolism are closely related to the maturation process.
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology*; D000242:Cyclic AMP / Q000494:pharmacology; D005260:Female; D066298:In Vitro Techniques; D008540:Meiosis; D009865:Oocytes / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D010063:Ovum / Q000502:physiology*; D010101:Oxygen Consumption; D051381:Rats
D003994:Bucladesine; D000242:Cyclic AMP