title: Separation and infectivity of two particle types of human rotavirus.
abstract: Two morphological types of human rotavirus particles were separated in caesium chloride density gradients. The particles of higher density ('incomplete' particles without an outer capsid layer) banded at a density of 1-38 g/ml, while the less dense ('complete' particles with an outer as well as an inner capsid layer) banded at a density of 1-36 g/ml. Some particles were found with an incomplete outer layer of capsomeres. The particle/infectivity ratio for tissue cultures of the fraction containing complete particles was more than 3 log10 higher than that of the fraction containing incomplete particles. But, as there was a small number of complete particles in the fraction containing mostly incomplete particles, it was impossible to determine whether the incomplete particles had a low infectivity or whether they had none at all.
mesh_terms: D002213:Capsid / Q000032:analysis; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D002648:Child; D003967:Diarrhea / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D006801:Humans; D012328:RNA Viruses / Q000302:isolation & purification; D012401:Rotavirus / Q000302:isolation & purification / Q000472:pathogenicity / Q000648:ultrastructure; D014771:Virion / Q000302:isolation & purification / Q000472:pathogenicity / Q000648:ultrastructure