Banded karyotypes of H-4-IIE-C3 rat hepatoma cells grown in vitro.
Mitotic cells from the H-4-IIE-C3 rat hepatoma tissue culture line showed a range of 45 to 53 chromosomes per cell with 75% of the cells displaying a chromosome mumber between 49 and 52. Analysis of Wright's-Giemsa banded karyotypes of 22 cells revealed considerable cell to cell variation. Twenty-one structurally abnormal chromosomes were identified in these cells; the origin of nine of the 21 chromosomes were identified in these cells; the origin of nine of the 21 chromosomes could be determined. Of the structurally abnormal chromosomes detected, only one (M-1) occurred with a sufficiently high frequency to be of general use as a marker for these cells. This marker appears to be a Robertsonian translocation involving chromosome number 2 and chromosome number 10.
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D002460:Cell Line; D002869:Chromosome Aberrations; D002875:Chromosomes; D066298:In Vitro Techniques; D007621:Karyotyping; D008113:Liver Neoplasms / Q000235:genetics*; D008297:Male; D051381:Rats