The effect of diabetes and insulin on adipose lactate dehydrogenase isozymes.
Adipose lactate dehydrogenase (LDH) (EC 1.1.1.27) isozyme distribution was altered in streptozotocin-diabetic and fasting rats resulting from a relative reduction of subunit A. Treatment with insulin for 2 days partially restored the relative content of isozyme 5 to control values in the diabetic rats, and the effect of insulin was not inhibited by simultaneous injection of actinomycin D or puromycin. When the epididymal adipose tissues isolated from control animals were incubated in vitro with dibutyryl adenosine 3',5'-cyclic monophosphate or epinephrine, a relative decrease in subunit A was observed; whereas, either compound caused an increase in subunit A in diabetic tissues. The findings suggest that the redistribution of LDH isozyme under these conditions is to prevent excessive accumulation of lactate in the tissue.
D000273:Adipose Tissue / Q000187:drug effects / Q000201:enzymology*; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D003994:Bucladesine / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D003920:Diabetes Mellitus / Q000139:chemically induced / Q000201:enzymology*; D004837:Epinephrine / Q000494:pharmacology; D005215:Fasting; D007328:Insulin / Q000494:pharmacology*; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D051381:Rats; D013311:Streptozocin
D001786:Blood Glucose; D007328:Insulin; D007527:Isoenzymes; D003609:Dactinomycin; D013311:Streptozocin; D003994:Bucladesine; D007770:L-Lactate Dehydrogenase; D004837:Epinephrine