title: Solid phase indirect radioimmunoassay: standardization and applications in viral serology.
abstract: In solid phase indirect radioimmunoassay (IRIA) antiviral immunoglobulins (Ig) bind specifically to the viral antigen fixed onto wells of microtiter plates. Radioactively labeled (125I) anti-Ig can be used to detect these antiviral antibodies. The sensitivity of the IRIA depends on the amount of antigen in the microtiter wells and the concentration of the 125I-labeled anti-Ig used. To standardize the assay, a method of antigen titration in the IRIA was devised, using parainfluenza type 1 viruses as antigens. The IRIA provides a means to titrate viral antigens with different biologic activities and antibodies against them. A binding inhibition test (BIT) based on IRIA allows the antigenic analysis of different virus strains, as is demonstrated for two closely related parainfluenza type 1 virus strains.
mesh_terms: D000888:Antibodies, Anti-Idiotypic; D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral; D004306:Dose-Response Relationship, Immunologic; D010222:Parainfluenza Virus 1, Human / Q000276:immunology*; D011863:Radioimmunoassay / Q000379:methods
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000914:Antibodies, Viral; D000956:Antigens, Viral