Analysis of DNA structure by hydroxyapatite columns and ethidium bromide fluorescence techniques. A comparative study and effect on DNA binding.
Seven duplex DNA preparations have been structurally analyzed by hydroxyapatite column chromatography and an ethidium bromide fluorescence technique. Significant contamination of one preparation with single-stranded DNA was detected by hydroxyapatite column chromatography. Five of the other six preparations were found to contain significant single-stranded regions by the ethidium bromide fluorescence technique. Synthetic poly dAT was found to be duplex in structure. The presence of single-stranded regions considerably influenced DNA binding results in a radioimmunoassay.
D000906:Antibodies; D000918:Antibody Specificity; D002845:Chromatography / Q000379:methods*; D004247:DNA / Q000032:analysis* / Q000276:immunology; D004265:DNA Helicases; D004261:DNA Replication; D004277:DNA, Single-Stranded / Q000032:analysis; D004996:Ethidium; D006801:Humans; D006882:Hydroxyapatites; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D008968:Molecular Conformation; D011071:Poly T / Q000032:analysis; D011485:Protein Binding; D011863:Radioimmunoassay / Q000379:methods*
D000906:Antibodies; D004277:DNA, Single-Stranded; D006882:Hydroxyapatites; D011071:Poly T; D004247:DNA; D004265:DNA Helicases; D004996:Ethidium