The isozyme pattern of cyclic AMP-dependent protein kinase and the distribution of a cervicovaginal antigen in experimental carcinoma of the cervix uteri of mice.
Tissue-sections from 12 methylcholanthrene-induced carcinomas of the cervix uteri of mice were tested for the presence of an antigen normally confined to the cervicovaginal epithelium. The antigen was detected in 10 of the 12 tumours investigated with indirect immunofluorescence, and in all 7 tumours studied with the more sensitive method of mixed hemagglutination. The concentration of the antigen was generally higher in the well-differentiated areas of the tumours, but it was also found associated with solitary tumour cells, apparently invading the stroma. The presence of CVA in the tumours suggests an origin of the tumour cells from the cervicovaginal epithelium. The cyclic AMP dependent protein kinase (EC 2. 7. 1. 37) in the tumour cytosols was studied by chromatography on agarose and DEAE-cellulose. The enzyme showed the same properties as that from normal vaginal epithelium. The tumour cells thus contain an apparently normal complement of this enzyme, which is believed to be responsible for most of the intracellular actions of cyclic AMP.
D000818:Animals; D000941:Antigens / Q000032:analysis; D002584:Cervix Uteri / Q000276:immunology*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D000242:Cyclic AMP; D003600:Cytosol / Q000201:enzymology; D004848:Epithelium / Q000276:immunology; D005260:Female; D005455:Fluorescent Antibody Technique; D006386:Hemagglutination Tests; D007527:Isoenzymes / Q000378:metabolism*; D051379:Mice; D009374:Neoplasms, Experimental / Q000201:enzymology / Q000276:immunology / Q000473:pathology; D011494:Protein Kinases / Q000378:metabolism*; D002583:Uterine Cervical Neoplasms / Q000201:enzymology / Q000276:immunology / Q000473:pathology; D014621:Vagina / Q000276:immunology*
D000941:Antigens; D007527:Isoenzymes; D000242:Cyclic AMP; D011494:Protein Kinases