Citrobacter O-antigens: structure of the O-antigenic polysaccharide from Citrobacter sp. 396.
The structure of the O-specific polysaccharide moiety of the lipopolysaccharide from Citrobacter 396 was elucidated by composition, methylation, and periodate oxidation studies. The repeating unit consists of four 2-linked mannoses and one 3-linked N-acetylglucosamine. One of the mannose units is substituted at C3 with alpha-glucose, and one is substituted at C3 with alpha-(2-O-acetyl)-abequose. All the mannosyl linkages appear to have the beta-configuration; the N-acetylglucosaminyl linkage has the alpha-configuration. In bacterial agglutination and passive hemagglutination in some Salmonella antisera, Citrobacter 396 as well as its O-antigenic lipopolysaccharide expressed the serological factors 5 and 6. In corroboration of our structural studies, this showed the presence of alpha-(2-O-acetyl)-abequosyl-1,3-mannose (factor 5) and alpha-glucosyl-1,3-mannose (factor 6).
D000942:Antigens, Bacterial / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D002954:Citrobacter / Q000032:analysis / Q000276:immunology*; D003429:Cross Reactions; D004755:Enterobacteriaceae / Q000276:immunology*; D008070:Lipopolysaccharides / Q000032:analysis / Q000276:immunology*; D008745:Methylation; D010504:Periodic Acid; D011135:Polysaccharides, Bacterial / Q000032:analysis / Q000276:immunology*
D000942:Antigens, Bacterial; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D010504:Periodic Acid