Appearance of early and late components of Epstein-Barr virus-associated membrane antigen in Daudi cells superinfected with P3HR-1 virus.
This study investigated the synthesis of membrane antigen (MA) as well as virus capsid antigen (VCA) and early antigen (EA) in Daudi cells which had been superinfected with the P3HR-1 strain of Epstein-Barr virus (EBV) and then treated with trypsin to remove initially absorbed MA-positive material from the cell surface. Synthesis of MA, VCA and EA was completely inhibited by puromycin. A marked reduction in the frequency of MA positive cells was observed in superinfected cells cultured in the presence of either cytosine arabinoside (Ara-C) or phosphonoacetate (PA); however, a small fraction of MA synthesis occurred, suggesting an inhibitor insensitive component in MA, A differential absorption of EBV antibody-positive human serum with the Ara-C treated or untreated infected cells detected two antigenically different components in MA: early (Ara-C insensitive) and late (Ara-C sensitive) MA.
D000956:Antigens, Viral / Q000032:analysis; D002051:Burkitt Lymphoma; D002213:Capsid / Q000276:immunology; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D003561:Cytarabine / Q000494:pharmacology; D004854:Herpesvirus 4, Human / Q000254:growth & development / Q000276:immunology*; D010746:Phosphonoacetic Acid / Q000494:pharmacology; D011691:Puromycin / Q000494:pharmacology; D014357:Trypsin / Q000494:pharmacology
D000956:Antigens, Viral; D003561:Cytarabine; D011691:Puromycin; D014357:Trypsin; D010746:Phosphonoacetic Acid