Steroid hormone alteration of herpes simplex virus type 1 replication.
The effect of steroid hormones on herpes simplex virus type 1 replication was examined. Virus replication studies revealed that various concentrations of prednisolone, hydrocortisone, dexamethasone, or progesterone could decrease virus yields up to a maximum of 99%. Using isopycnic centrifugation in CsCl to separate viral from cell DNA, it was found that virus-specific DNA synthesis was decreased by 30 to 100% depending on the hormone and concentration used. Cell-specific DNA synthesis was also adversely affected, but this did not alter cell viability or plating efficiency.
D002460:Cell Line; D004273:DNA, Neoplasm / Q000096:biosynthesis; D004279:DNA, Viral / Q000096:biosynthesis; D003907:Dexamethasone / Q000494:pharmacology; D006728:Hormones / Q000494:pharmacology*; D006854:Hydrocortisone / Q000494:pharmacology; D011239:Prednisolone / Q000494:pharmacology; D011374:Progesterone / Q000494:pharmacology; D018139:Simplexvirus / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D013256:Steroids / Q000494:pharmacology*; D014779:Virus Replication / Q000187:drug effects*
D004273:DNA, Neoplasm; D004279:DNA, Viral; D006728:Hormones; D013256:Steroids; D011374:Progesterone; D003907:Dexamethasone; D011239:Prednisolone; D006854:Hydrocortisone