Separation of naphthoquinones and lipophilic vitamins by gel and thin-layer chromatography.
A separation of naphthoquinones on silica gel and on silica gel impregnated with polyethylene glycol 200 by thin-layer chromatography was compared with gel permeation chromatography (GPC) on styrene-divinylbenzene copolymer S-832-gel using tetrahydrofuran as mobile phase. Factors affecting the separations attainable are discussed, and it is concluded that GPC is a suitable method for the determination of K vitamins in natural materials.
D002850:Chromatography, Gel; D002855:Chromatography, Thin Layer; D004872:Ergocalciferols / Q000302:isolation & purification; D005782:Gels; D008722:Methods; D009285:Naphthoquinones / Q000302:isolation & purification*; D012822:Silicon Dioxide; D014812:Vitamin K / Q000302:isolation & purification*
D004872:Ergocalciferols; D005782:Gels; D009285:Naphthoquinones; D014812:Vitamin K; D012822:Silicon Dioxide