title: Rapid detection of human cytomegalovirus and herpesvirus hominis IgM antibody by the immunoperoxidase technique.
abstract: Immunoglobulin M (IgM) to human cytomegalovirus (CMV) and herpesvirus hominis (HVH, types 1 and 2) has been searched for in a group of patients with congenital and recent CMV and HVH infections and also in a group of patients with remote CMV and recurrent HVH infections, using the immunoperoxidase antibody technique (IPA) as compared to a technique using sucrose density gradient differential centrifugation. Both techniques detected the same positive samples. High IgM antibody titers (1:32-1:512) were observed in all cases of primary CMV and HVH infection, whereas low titers (1:8-1:16) were found in congenital infections. The IPA technique is sensitive, specific and useful for rapid diagnosis of recent and congenital CMV and HVH infections.
mesh_terms: D000328:Adult; D000914:Antibodies, Viral / Q000032:analysis*; D002478:Cells, Cultured; D003429:Cross Reactions; D003587:Cytomegalovirus / Q000276:immunology*; D003586:Cytomegalovirus Infections / Q000151:congenital / Q000276:immunology*; D006561:Herpes Simplex / Q000276:immunology*; D006801:Humans; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007223:Infant; D007231:Infant, Newborn; D018139:Simplexvirus / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M