Isolation of polyoma virus-induced surface antigens in hamster cells: potassium chloride solubilization and differential precipitation.
Surface antigens were extracted in soluble and active form with 3 M KCL from polyoma virus-transformed and embryonic hamster cells. These were tumor-specific transplantation antigens (TSTA), shown by tumor rejection, and a surface (S) antigen, demonstrated by the inhibition of surface fluorescence on living polyoma virus-transformed cells. The extracts were fractionated by salting out with ammonium sulfate. In tumor cell extracts, all TSTA activity and a part of S antigen activity were found in the fraction precipitated with 60% saturation in (NH/)2SO4. Another part of S antigen activity was found in the fraction precipitating at 80% saturation in tumor cell extracts. The precipitate at 60% saturation of embryonic cell extracts also had a part of S antigen activity. Receptor site activity for concanavalin A was also retained after solubilization and was confined to the 40% saturation (NH4)2SO4 precipitate in the case of tumor and embryonic cell extracts.
D000818:Animals; D000951:Antigens, Neoplasm / Q000302:isolation & purification*; D000956:Antigens, Viral / Q000302:isolation & purification*; D002462:Cell Membrane / Q000276:immunology; D002471:Cell Transformation, Neoplastic; D006224:Cricetinae; D004622:Embryo, Mammalian / Q000276:immunology; D005354:Fibrosarcoma / Q000276:immunology; D005590:Fractional Precipitation; D006649:Histocompatibility Antigens / Q000302:isolation & purification*; D008647:Mesocricetus; D051379:Mice; D008809:Mice, Inbred C3H; D009374:Neoplasms, Experimental / Q000276:immunology; D011120:Polyomavirus / Q000276:immunology*; D011189:Potassium Chloride; D012995:Solubility
D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D006649:Histocompatibility Antigens; D011189:Potassium Chloride