Protein A-peroxidase: a valluable tool for the localization of antigens.
Protein A of Staphylococcus aureus has been conjugated to horseradish peroxidase and used in an indirect immunolabeling technique to visualize membrane and viral antigens. The same Protein A-peroxidase conjugate was used with antisera from five different species. Using this indirect test, membrane markers for T and B lymphocytes were labeled with a greater specificity than when peroxidase conjugated anti-immunoglobulin was used in the second step. Viral antigens on cells infected with measles, vesicular stomatitis, herpes or visna virus, respectively, were also stained in the protein A-peroxidase indirect test with a greater specificity than indirect method using anti-immunoglobulin. Paired preparations were examined in the light and electron microscope. Ultrastructural analysis showed that the protein A-peroxidase conjugate penetrated well through fixed viral membranes and resulted in fine resolution of antigenic sites.
D000818:Animals; D000941:Antigens / Q000032:analysis*; D001426:Bacterial Proteins; D002460:Cell Line; D002472:Cell Transformation, Viral; D006735:Horseradish Peroxidase; D006801:Humans; D007124:Immunoenzyme Techniques; D051379:Mice; D008854:Microscopy, Electron / Q000379:methods; D010544:Peroxidases; D011817:Rabbits / Q000276:immunology; D018139:Simplexvirus; D013211:Staphylococcus aureus; D013950:Thymus Gland / Q000276:immunology / Q000648:ultrastructure; D014721:Vesicular stomatitis Indiana virus
D000941:Antigens; D001426:Bacterial Proteins; D006735:Horseradish Peroxidase; D010544:Peroxidases