A simple spectrophotometric method for estimation of plasma angiotensin I converting enzyme activity.
The procedure described is rapid, fairly simple, and inexpensive. The method may easily be used even in a small clinical laboratory. This method, based on a specific reaction which gives a product, hippuric acid azlactone, which absorbs in the visible region, avoids the interference of reagents and solvents encountered in the UV spectrophotometric assay, in which hippuric acid is determined directly.
D000818:Animals; D006626:Hippurates / Q000097:blood; D006801:Humans; D009842:Oligopeptides / Q000097:blood; D007703:Peptidyl-Dipeptidase A / Q000097:blood*; D011817:Rabbits; D051381:Rats; D013045:Species Specificity; D013053:Spectrophotometry / Q000379:methods
D006626:Hippurates; D009842:Oligopeptides; D007703:Peptidyl-Dipeptidase A