Quantitative in vivo studies of soluble simian virus 40 tumor-specific transplantation antigens of the mouse.
Quantitative studies have been performed on the immunogenicity of a membrane-bound antigen of a simian virus 40 (SV40) -induced sarcoma in syngeneic BALB/c mice and of subcellular fractions derived from this tumor. The objectives of the investigation were: a) to develop a quantitative in vivo assay of the tumor-specific transplantation antigen (TSTA) and b) to compare the distribution of histocompatibility antigens, H-2, with that of the SV40 TSTA during several fractionation steps. The immunogenicity of the TSTA-containing fractions was assessed from dose-response curves relating tumor size and the amount of protein used for immunization. After digestion of the tumor cell membranes with a limited amount of papain, H-2 as well as TSTA were present in a soluble form. A single immunization with only 2 microng of the solubilized TSTA reduced the tumor size by 70% compared to that in nonimmunized control animals. The results of several fractionation steps suggest that H-2 and the TSTA are not tightly associated in the solubilized immunogenic material.
D000818:Animals; D000951:Antigens, Neoplasm / Q000008:administration & dosage / Q000032:analysis*; D002462:Cell Membrane / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D006649:Histocompatibility Antigens / Q000032:analysis*; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D012513:Sarcoma, Experimental / Q000276:immunology*; D013539:Simian virus 40 / Q000276:immunology*; D014185:Transplantation, Isogeneic; D014412:Tumor Virus Infections / Q000276:immunology
D000951:Antigens, Neoplasm; D006649:Histocompatibility Antigens