The effects of ascorbic acid on diphtheria toxin and intoxicated HeLa cells.
Ascorbic acid (vitamin C) prevented diphtheria toxin from inhibiting the incorporation of [U-14C]-alanine into trichloroacetic acid precipitable material in HeLa cells. Ascorbic acid did not exhibit an effect on the adenosine diphosphate-ribosylation of amino acyl transferase II nor did it separate fragment A from fragment B in "nicked" toxin. A non-specific reducing agent, para-methylaminophenol sulfate, exhibited an effect on HeLa cells very similar to the results of ascorbic acid. Citric acid, a tricarboxylic acid, had no effect on HeLa cells.
D000409:Alanine / Q000378:metabolism; D001205:Ascorbic Acid / Q000494:pharmacology*; D002951:Citrates / Q000494:pharmacology; D004167:Diphtheria Toxin / Q000494:pharmacology*; D006367:HeLa Cells / Q000187:drug effects / Q000378:metabolism*; D009243:NAD / Q000494:pharmacology; D009363:Neoplasm Proteins / Q000096:biosynthesis; D009713:Nucleotidyltransferases / Q000378:metabolism; D010445:Peptide Elongation Factors
D002951:Citrates; D004167:Diphtheria Toxin; D009363:Neoplasm Proteins; D010445:Peptide Elongation Factors; D009243:NAD; D009713:Nucleotidyltransferases; D000409:Alanine; D001205:Ascorbic Acid