title: Gas-liquid chromatographic determination of residues of methanesulfonate of n-aminobenzoic acid ethyl ester in fish.
abstract: In this gas-liquid chromatographic procedure for determining residues of methanesulfonate of m-aminobenzoic acid ethyl ester (MS-222) in fish muscle, homogenized tissue is extracted with distilled water, and proteins are removed by coagulation with trichloroacetic acid, centrifugation, and filtration. After careful pH adjustment of the filtrate, MS-222 is partitioned into benzene-ethyl ether and measured by alkali flame ionization gas chromatography. Tissues with known additions of 1-19 microgram MS-222/g were analyzed, with recoveries of 84-95%.
mesh_terms: D062365:Aminobenzoates / Q000032:analysis*; D000818:Animals; D002849:Chromatography, Gas; D003124:Colorimetry; D005399:Fishes / Q000378:metabolism*; D008698:Mesylates / Q000032:analysis*; D008722:Methods
chemical_list: D062365:Aminobenzoates; D008698:Mesylates