Retrovirus purification: method that conserves envelope glycoprotein and maximizes infectivity.
A Sepharose 4B chromatographic method for purification of retroviruses is described which was less time consuming, increased purified virus yields, conserved viral glycoprotein, and increased recovery of biological infectivity in comparison with conventional sucrose gradient ultracentrifugation techniques.
D000406:AKR murine leukemia virus / Q000032:analysis / Q000254:growth & development / Q000302:isolation & purification*; D002499:Centrifugation, Density Gradient; D002847:Chromatography, Agarose / Q000379:methods*; D002850:Chromatography, Gel / Q000379:methods*; D017988:Gammaretrovirus / Q000032:analysis / Q000254:growth & development / Q000302:isolation & purification*; D006023:Glycoproteins / Q000032:analysis; D009052:Leukemia Virus, Murine / Q000302:isolation & purification*; D010455:Peptides / Q000032:analysis; D010948:Viral Plaque Assay; D014764:Viral Proteins / Q000032:analysis
D006023:Glycoproteins; D010455:Peptides; D014764:Viral Proteins