title: The induction of tumors in Rana temporaria with nitrosamines.
abstract: Dimethylnitrosamine (DMNA) and diethylnitrosamine (DENA) dissolved in tank water in a dose of 5 and 50 ppm, resp. induced tumors of liver (hepatocellular cancers and adenomas), and of hematopoietic system (hemocytoblastosis). DMNA induced neoplasms in 19 of 43 frogs (44.2%); the average latent period being 18.2 weeks, while DENA induced tumors in 41 of 94 animals (43.6%) within a mean latent period 19.7 weeks. Results of this investigation show the possibility to use anuran amphibians in shorttermed experiments in the study of nitrosocompounds carcinogenicity as well as a biological indicator for environmental pollution.
mesh_terms: D000236:Adenoma / Q000139:chemically induced*; D000818:Animals; D001001:Anura; D006528:Carcinoma, Hepatocellular / Q000139:chemically induced*; D004052:Diethylnitrosamine / Q000008:administration & dosage; D004128:Dimethylnitrosamine / Q000008:administration & dosage; D006413:Hematopoietic System; D008113:Liver Neoplasms / Q000139:chemically induced*; D009374:Neoplasms, Experimental / Q000139:chemically induced; D009602:Nitrosamines / Q000008:administration & dosage; D011896:Rana temporaria; D013997:Time Factors
chemical_list: D009602:Nitrosamines; D004052:Diethylnitrosamine; D004128:Dimethylnitrosamine