Modification of theophylline-induced lipolysis in human fat cells after trypsination.
Trypsin-treatment of human fat cells results in the potentiation of the lipolytic response and the cAMP accumulation induced by theophylline (5 . 10(-4) M) but not of those induced by theophylline (5 . 10(-3) M). The amount of cAMP formed after exposure to theophylline (5 . 10(-3) M) plus norepinephrine (5 . 10(-6) M) remains, however, 2.6 fold higher in trypsin-treated human fat cells than in the control ones.
D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism*; D000242:Cyclic AMP / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D050356:Lipid Metabolism; D009638:Norepinephrine / Q000494:pharmacology; D013806:Theophylline / Q000494:pharmacology*; D013997:Time Factors; D014357:Trypsin / Q000494:pharmacology*
D013806:Theophylline; D000242:Cyclic AMP; D014357:Trypsin; D005990:Glycerol; D009638:Norepinephrine