[Compared expression of murine plasmacytoma associated virus in tumor cells and in mouse embryo cells].
A DNA complementary to the viral genome of C-type particles produced by a Mouse myeloma derived cell line (MF2 cell line) was synthesized. This cDNA was used as a probe to study the viral genome expression among the total RNA and the poly (A)-rich RNA extracted from the MF2 and Balb/c embryonic cells. As evidenced by molecular hybridization experiments, the presence of at least one endogenous Balb/c virus in the MF2 virus stocks is suggested. In the productive cells, the viral RNA sequences are expressed in the poly (A)-rich RNA fraction.
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002478:Cells, Cultured; D004279:DNA, Viral / Q000096:biosynthesis; D004622:Embryo, Mammalian; D017988:Gammaretrovirus / Q000378:metabolism*; D051379:Mice; D009693:Nucleic Acid Hybridization; D010954:Plasmacytoma / Q000382:microbiology*; D011061:Poly A / Q000096:biosynthesis; D012367:RNA, Viral / Q000096:biosynthesis; D014158:Transcription, Genetic
D004279:DNA, Viral; D012367:RNA, Viral; D011061:Poly A
Expression comparée du virus associé au plasmocytome murin dans les cellules tumorales et dans les cellules de la souche murine d'origine.