Solid-phase radioimmunoassay of herpes simplex virus IgG and IgM antibodies.
A solid-phase radioimmunoassay for detection of herpes simplex virus-specific IgG and IgM antibodies in human serum specimens is presented. Virus antigen is adsorbed on polystyrene balls and antibodies which attach to the antigen are detected by 125I-labeled antihuman-gamma or antihuman-mu immunoglobulins. A total of 76 specimens have been tested. The appearance of virus-specific IgG and IgM antibodies in primary herpetic infections was readily demonstrated. When serum samples from patients with past exposure to herpes simplex virus were tested, endpoint titers of virus-specific IgG antibodies were found to be 8 to 2048 times higher than titers determined by a complement fixation test. Apparent cross reactivity with varicella-zoster virus was observed in the present radioimmunoassay.
D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral; D006561:Herpes Simplex / Q000276:immunology; D006562:Herpes Zoster / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins / Q000378:metabolism; D007163:Immunosorbent Techniques; D007457:Iodine Radioisotopes; D011863:Radioimmunoassay; D018139:Simplexvirus / Q000276:immunology*; D013696:Temperature; D013997:Time Factors
D000914:Antibodies, Viral; D000956:Antigens, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins; D007457:Iodine Radioisotopes