Screening of anti-B cell antibodies in the sera of renal transplant recipients by using Daudi cell line.
In order to examine the presence of antibodies against B cells, 83 sera obtained from 55 renal transplant recipients were screened by microcytotoxicity test using Daudi cell line. Out of 83 samples, 12 were pretransplant sera, 62 were post-transplant sera and 9 were from the patients whose grafts has been removed due to rejection. For the purpose of improving the reliability of the tests, 5 different rabbit complements were selected and cytotoxicity tests with them were carried out simultaneously. Pretransplant sera without blood transfusion did not show any cytotoxicity against Daudi cells. Although 42% of post-transplant sera showed cytotoxicity against Daudi cells, cytotoxic activity of the sera showed no relation with the occurrence of rejection episodes. Three serum samples out of 5 patients whose grafts had been removed due to rejection showed strong cytotoxicity against Daudi cells and the sera of 2 other patients did not. There was no relation between the cytotoxicity against Daudi cells and antibodies against Epstein-Barr virus capsid antigen in the post-transplant sera.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000914:Antibodies, Viral / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D002277:Carcinoma / Q000276:immunology; D002460:Cell Line; D003601:Cytotoxicity Tests, Immunologic; D006084:Graft Rejection; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D016030:Kidney Transplantation; D009303:Nasopharyngeal Neoplasms / Q000276:immunology; D011817:Rabbits
D000906:Antibodies; D000914:Antibodies, Viral; D000956:Antigens, Viral