Purification of beta-toxin from Clostridium perfringens type C.
Beta-toxin was purified about 340-fold from culture supernatant fluid of Clostridium perfringens type C with a yield of about 24% in terms of biologically active beta-toxin. The purification involved ammonium sulfate fractionation, gel filtration through Sephadex G-100, isoelectrofocusing in a pH 3 to 6 gradient, and immunoaffinity chromatography. The purified beta-toxin gave a single band on polyacrylamide gel electrophoresis.
D001427:Bacterial Toxins / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D003016:Clostridium perfringens; D004591:Electrophoresis, Polyacrylamide Gel; D007525:Isoelectric Focusing
D001427:Bacterial Toxins