Identification of 2-methyl-3-hydroxydecanoic and 2-methyl-3-hydroxytetradecanoic acids in the 'lipid X' fraction of the Bordetella pertussis endotoxin.
The 'lipid X' fraction, released from the Bordetella pertussis endotoxin upon treatment with trifluoroacetic acid of pH 3 at 50 degrees C, was shown to contain, in addition to 3-hydroxydecanoic, 3-hydroxydodecanoic, 3-hydroxytetradecanoic, and tetradec-2-enoic acids, 2-methyl-3-hydroxydecanoic-, and 2-methyl-3-hydroxytetradecanoic acids. The structure of these was established by gas-liquid chromatography/mass spectrometry.
D001886:Bordetella pertussis / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D002849:Chromatography, Gas; D004731:Endotoxins / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D006880:Hydroxy Acids / Q000032:analysis; D007700:Kinetics; D008055:Lipids / Q000032:analysis*; D013058:Mass Spectrometry
D004731:Endotoxins; D005227:Fatty Acids; D006880:Hydroxy Acids; D008055:Lipids