Effects of concanavalin A and neuraminidase on cyclic AMP levels and 14C-1-glucose oxidation in dog thyroid slices.
Treatment with concanavalin A at 100 micron/ml or higher concentrations significantly increased 14C-1-glucose oxidation in dog thyroid slices as reported in other tissues. This treatment exerted no effect on tissue cyclic AMP levels. Neuraminidase at the same concentrations also had similar effects on these parameters. Neither concanavalin A nor neuraminidase at the concentrations up to 100 microng/ml had the TSH effect on both tissue cyclic AMP and 14C-1-glucose oxidation. These results indicate that modification of carbohydrate moieties of glycoproteins on the cell surface may cause an increase in glucose metabolism without any critical effect on cyclic AMP system and in the process of TSH response.
D000818:Animals; D002250:Carbon Radioisotopes; D003016:Clostridium perfringens / Q000201:enzymology; D003208:Concanavalin A / Q000494:pharmacology*; D000242:Cyclic AMP / Q000096:biosynthesis*; D004285:Dogs; D005947:Glucose / Q000378:metabolism*; D009439:Neuraminidase / Q000494:pharmacology*; D010084:Oxidation-Reduction; D013961:Thyroid Gland / Q000378:metabolism*; D013972:Thyrotropin / Q000494:pharmacology
D002250:Carbon Radioisotopes; D003208:Concanavalin A; D013972:Thyrotropin; D000242:Cyclic AMP; D009439:Neuraminidase; D005947:Glucose