The mechanism of action of citrinin on rabbit kidney alkaline phosphatase activity in vivo.
The effect of citrinin poisoning on rabbit kidney alkaline phosphatase was investigated. After seven days administration of citrinin (2 mg/kg body weight daily) the animals were sacrificed and the level of enzymes estimated in serum and kidney. Serum enzymes showed no variation in activity in the citrinin-treated animals, but in kidney, alkaline phosphatase activity decreased significantly. The decreased activity was mainly associated with the cytoplasmic fraction and in fractions Ib and II. The enzyme II obtained from citrinin-treated animal showed no kinetic difference in substrate specificity, inhibition by phenylalanine, phosphate, sodium-EDTA and Zn2+ ions, activation by Mg2+ ions, thermal inactivation and electrophoretic mobility to that of control Enzyme II. Immunological studies showed that the decrease in enzyme activity was due to existence of inactive enzyme protein. Hormones like cyclic AMP, prostaglandin E1 and parathyroid hormone reversed the decreased enzyme activity due to citrinin poisoning in mouse and rabbit. This study favours the possible existence of active and inactive forms of alkaline phosphatase in the system.
D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D000937:Antigen-Antibody Reactions; D001230:Aspergillus; D001578:Benzopyrans / Q000494:pharmacology*; D003994:Bucladesine / Q000494:pharmacology; D002953:Citrinin / Q000494:pharmacology*; D004798:Enzymes / Q000097:blood; D005260:Female; D007527:Isoenzymes / Q000378:metabolism; D007668:Kidney / Q000187:drug effects / Q000201:enzymology*; D051379:Mice; D010281:Parathyroid Hormone / Q000494:pharmacology; D011453:Prostaglandins / Q000494:pharmacology; D011817:Rabbits; D013045:Species Specificity
D001578:Benzopyrans; D004798:Enzymes; D007527:Isoenzymes; D010281:Parathyroid Hormone; D011453:Prostaglandins; D002953:Citrinin; D003994:Bucladesine; D000469:Alkaline Phosphatase