[Homogeneous Cl. perfringens alpha-anatoxin: its physicochemical and immunogenic properties].
The authors present a method of obtaining relatively homogeneous preparations of alpha-toxoid of Cl. perfringens, type A, including the primary conception of the alpha-toxin proteins, their chromatography on DEAE-cellulose, fractionation with (NH4)2SO4, detoxication, with the subsequent gel-filtration through sephadex and isoelectric focussing. Sedimentation coefficient of the preparation proved to be 3.8 S, isoelectric point-4.83 +/- 0.07. In studying the immunogenic properties of alpha-toxoid in experiments on guinea pigs and rabbits their high immunogenicity, exceeding that of the industrial toxoid 8- and 6-fold, respectively, was established. Homogeneous preparations of alpha-toxoid provided intense anti-microbial immunity. Interlinear differences in the levels of the immune response of inbred mice, highly-reactive (BALB/c) and low-reactive (C57BL/6) to alpha-toxoid, reached 20-fold; in combination with the high immunogenicity of this antigen for mice this permits to recommend it for immunogenic studies.
D000818:Animals; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003016:Clostridium perfringens / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D006168:Guinea Pigs; D007114:Immunization; D007117:Immunization, Secondary; D007525:Isoelectric Focusing; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D011817:Rabbits; D013997:Time Factors; D014121:Toxoids / Q000276:immunology / Q000302:isolation & purification*
D014121:Toxoids
Gomogennyĭ al'fa-anatoksin C1. perfringens: ego fiziko-khimicheskie i immunogennye svoĭstva.