[Prospects for use of the anterior chamber of the eye for studying proliferation, differentiation and hybridization of tumor cells].
The anterior chamber of mammalian eye (ACE) is one of the places of organism privileged immunologically that permits to use it for transplantations of allogenic tissues. Besides, the medium filling the chamber, provides an excellent growth of transplants there, whereas the transparent cornea allows to control the growth. The analysis of results obtained with this method shows its acceptibility both for heterotransplantations of tumors including human tumors, and for homotransplantations with the aim to study the progression of tumors and the selection of constant tumor lines. The ACE is easily applicable for studying morphogenetic potentialities of tumor cells, as well as their ability to differentiation and normalization, because its medium has the quality of "differentiating" for many tissues. Cytological heterogeneity, revealed during tumor transplantations, allows to study the spectrum of cytological variability and transgression of tumor characters at different stages of their progression. The ACE can be used for estimation of tumor formation ability of tumor cells by determining TD50 (tumor dosis 50) which is important for estimation of different kinds of damage (X-rays, chemotherapy etc.). According to authors' point of view, it is very perspective to use the anterior chamber of eye for elaboration of effective methods of hybridization tumor cells with normal ones.
D000818:Animals; D000867:Anterior Chamber; D006528:Carcinoma, Hepatocellular / Q000532:radiotherapy; D002454:Cell Differentiation; D046508:Culture Techniques; D006822:Hybrid Cells; D016030:Kidney Transplantation; D008113:Liver Neoplasms / Q000532:radiotherapy; D016031:Liver Transplantation; D008722:Methods; D008938:Mitosis; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000473:pathology*; D051381:Rats; D014184:Transplantation, Homologous; D014467:Ultraviolet Therapy
Perspektivy ispol'zovaniia peredneĭ kamery glaza dlia izucheniia proliferatsii, differentsiatsii i gibridizatsii opukholevykh kletok.