Purity of staphylococcal delta-haemolysin obtained by three different procedures.
Three methods at present available for the purification of staphylococcal delta-haemolysin were compared as to the purity and identify of the product obtained. None yielded a pure preparation of delta-haemolysin; one of the three preparations did not contain demonstrable delta-haemolysin when tested electrophoretically, but it contained deoxyribonuclease, penicillinase, phosphatase and alpha-haemolysin. The second preparation had delta-haemolysin activity and was free of alpha-haemolysin, but it contained lipase, egg-yolk factor, esterase, deoxyribonuclease, penicillinase, phosphatase and hyaluronidase. The third preparation contained all of the products mentioned above, except phosphatase, and it also contained alpha-haemolysin, staphylokinase, lysozyme and caseinase. These findings are discussed with special reference to the requirement for criteria of purity in work with staphylococcal products.
D003851:Deoxyribonucleases / Q000032:analysis; D010450:Endopeptidases / Q000032:analysis; D004950:Esterases / Q000032:analysis; D005069:Evaluation Studies as Topic; D006460:Hemolysin Proteins / Q000032:analysis / Q000302:isolation & purification*; D006821:Hyaluronoglucosaminidase / Q000032:analysis; D008049:Lipase / Q000032:analysis; D008722:Methods; D009113:Muramidase / Q000032:analysis; D010405:Penicillinase / Q000032:analysis; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis; D013211:Staphylococcus aureus / Q000032:analysis*
D006460:Hemolysin Proteins; D003851:Deoxyribonucleases; D004950:Esterases; D008049:Lipase; D010744:Phosphoric Monoester Hydrolases; D009113:Muramidase; D006821:Hyaluronoglucosaminidase; D010450:Endopeptidases; D010405:Penicillinase