Proteolytic activity of subcellular fractions from Streptomyces griseus no. 45-H.
Subcellular fractions were prepared from Streptomyces griseus No. 45-H at different stages of life cycle, and their proteolytic activity was examined. The highest proteolytic activity was found in the 24- and 72- h-old vegetative hyphae, the lowest in the resting spores. Spores contained about 9--30% of the proteolytic activity of vegetative cells. At the age of 16 h about 80%, at 26 h 70%, at 72 h 40%, and in spores about 60% of the proteolytic activity was particulate. The greatest part of the proteolytic activity could be inhibited by EDTA, lower levels of serine and sulfhydryl protease activities were detected in the cell-free extracts of vegetative cells.
D002731:4-Chloromercuribenzenesulfonate / Q000494:pharmacology; D002455:Cell Division; D004492:Edetic Acid / Q000494:pharmacology; D010447:Peptide Hydrolases / Q000378:metabolism*; D010664:Phenylmethylsulfonyl Fluoride / Q000494:pharmacology; D011480:Protease Inhibitors; D012270:Ribosomes / Q000378:metabolism; D013171:Spores, Bacterial / Q000201:enzymology; D013305:Streptomyces griseus / Q000201:enzymology* / Q000254:growth & development / Q000648:ultrastructure; D013347:Subcellular Fractions / Q000201:enzymology
D011480:Protease Inhibitors; D010664:Phenylmethylsulfonyl Fluoride; D002731:4-Chloromercuribenzenesulfonate; D004492:Edetic Acid; D010447:Peptide Hydrolases