Reactivity of antiinflammatory and superoxide dismutase active Cu(II)-salicylates.
The activity of chelated Cu(II) with four different aspirin-like drugs in various superoxide dismutase assays was examined. Prior to these studies the oxidation state of the involved copper was measured by x-ray photoelectron spectrometry and was found to be +II throughout. All copper complexes were able to suppress the xanthine-xanthine oxidase mediated reduction of both cytochrome c and nitroblue tetrazolium as well as the formazan formation by KO2 in a specific manner. The hydroxylation of benzo-[alpha]-pyrene as well as the demethylation of 7-ethoxycoumarin using induced hepatic rat microsomes could be successfully inhibited by the employed Cu(II) chelates. Cu(II)-acetylsalicylate was the most active copper complex. Our findings support the proposal that Cu(II) chelates are the active forms of aspirin-like antiinflammatory agents.
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001579:Benzopyrene Hydroxylase / Q000378:metabolism; D003300:Copper / Q000494:pharmacology*; D003374:Coumarins; D003574:Cytochrome c Group / Q000037:antagonists & inhibitors / Q000378:metabolism; D003640:Dealkylation; D003864:Depression, Chemical; D066298:In Vitro Techniques; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology; D009580:Nitroblue Tetrazolium / Q000037:antagonists & inhibitors / Q000378:metabolism; D010089:Oxidoreductases, N-Demethylating / Q000378:metabolism; D051381:Rats; D012459:Salicylates / Q000494:pharmacology*; D013052:Spectrometry, X-Ray Emission; D013482:Superoxide Dismutase / Q000378:metabolism*; D014969:Xanthine Oxidase / Q000378:metabolism; D014970:Xanthines / Q000378:metabolism
D000893:Anti-Inflammatory Agents; D003374:Coumarins; D003574:Cytochrome c Group; D012459:Salicylates; D014970:Xanthines; D009580:Nitroblue Tetrazolium; D003300:Copper; D001579:Benzopyrene Hydroxylase; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase; D010089:Oxidoreductases, N-Demethylating