Screening of mammary carcinoma for hormone dependency in vitro. Enzymatic activity in short-term organotypic cultures of breast biopsies from 62 patients.
Enzymatic activity in short-term organotypic cultures of breast biopsies from 62 patients. Am J Clin Pathol 67: 393-396, 1977. Mammary carcinomas from 62 patients were assessed for pentose shunt dehydrogenase activity initially and after 24-72 hours in organotypic cultures with or without exogenous hormones. Hormones tested were (1) estradiol, (2) testosterone, and (3) prolactin. Thirty-seven (60%) were judged hormone-independent, in vitro; 14 (23%) were judged hormone-dependent, in vitro; 11 (17%) were classed as "indeterminant." Clinical results of endocrine management of 13 cases and an appraisal of the usefulness of the method are presented.
D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000378:metabolism* / Q000628:therapy; D002277:Carcinoma / Q000378:metabolism* / Q000628:therapy; D002369:Castration; D005260:Female; D006651:Histocytochemistry; D006801:Humans; D008875:Middle Aged; D009362:Neoplasm Metastasis; D009924:Organ Culture Techniques; D010088:Oxidoreductases / Q000378:metabolism*; D011944:Receptors, Androgen; D011956:Receptors, Cell Surface; D013997:Time Factors
D011944:Receptors, Androgen; D011956:Receptors, Cell Surface; D010088:Oxidoreductases