Hormonal studies of uridine utilization in an insect cell line CP-1268 derived from the codling moth Laspeyresia pomonella.
Ecdysterone decreased cellular growth and the incorporation of uridine into RNA following 4 days of hormone exposure. This hormone did not affect uridine incorporation following short-term exposure up to 25 hours. Juvenile hormone and farnesol both significantly decreased uridine uptake and incorporation into RNA; however, uridine uptake was inhibited to a greater extent than uridine incorporation. Cyclic AMP increased the incorporation of uridine into RNA but had no demonstrable effect on the uptake process. This stimulation was not the result of cAMP degradation products. Cyclic AMP and ecdysterone together produced a significant increase in uridine incorporation into RNA. These studies demonstrate the potential utilization of insect cell lines for studying the mode of action of insect developmental hormones.
D002460:Cell Line; D000242:Cyclic AMP / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004441:Ecdysterone / Q000494:pharmacology; D005204:Farnesol / Q000494:pharmacology; D007301:Insect Hormones / Q000494:pharmacology*; D007447:Invertebrate Hormones / Q000494:pharmacology*; D007605:Juvenile Hormones / Q000494:pharmacology; D007915:Lepidoptera; D009036:Moths; D012313:RNA / Q000096:biosynthesis; D013806:Theophylline / Q000494:pharmacology; D014529:Uridine / Q000378:metabolism*
D007301:Insect Hormones; D007447:Invertebrate Hormones; D007605:Juvenile Hormones; D005204:Farnesol; D004441:Ecdysterone; D012313:RNA; D013806:Theophylline; D000242:Cyclic AMP; D014529:Uridine