Plasmid modification of radiation and chemical-mutagen sensitivity in Pseudomonas aeruginosa.
The R factor pMG2 protects Pseudomonas aeruginosa against the lethal effects of ultraviolet (u.v.) and gamma irradiation, and methyl methanesulphonate and N-methyl-N'-nitro-N-nitrosoguanidine treatment. Enhanced survival occurs in strains of uvr+ rec+ (wild-type) genotype and a variety of uvr rec+ type mutants. No protection occurs in a rec A-type mutant. The plasmid also enhances u.v.-induced mutagenesis. These effects appear to be due to host-cell controlled plasmid-determined DNA repair function(s). Studies on P. aeruginosa strains deficient in DNA polymerase I (polyA) suggest that a plasmid-determined repair resynthesis function may be responsible for increased u.v.-survival and enhanced u.v.-mutability in pMG2-containing bacteria.
D004256:DNA Polymerase I / Q000096:biosynthesis; D004260:DNA Repair; D004352:Drug Resistance, Microbial; D005720:Gamma Rays; D008698:Mesylates / Q000494:pharmacology*; D008741:Methyl Methanesulfonate / Q000494:pharmacology*; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology*; D009153:Mutagens / Q000494:pharmacology*; D009154:Mutation; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D011815:R Factors; D011839:Radiation, Ionizing; D011995:Recombination, Genetic; D014466:Ultraviolet Rays
D008698:Mesylates; D009153:Mutagens; D008769:Methylnitronitrosoguanidine; D008741:Methyl Methanesulfonate; D004256:DNA Polymerase I