[Functional activity of rat adenohypophysis cells in a primary monolayer culture and the effect of several regulators on it].
Some peculiarities of labeled growth hormone (GH) and prolactin (PL) secretion in the 5-day monolayer culture of the rat adenohypophysis was studied. The hormones from the culture medium were obtained by electrophoresis on polyacrylamide gel. Natrium-dibutyril of cyclic adenosine-3',5'-monophosphate and theophylline, stimulated the GH and PL secretion. Thyrotropin-releasing hormone (TRH) increased the incorporation of 14C-1-leucine into the cell protein, stimulated PL secretion, but did not act on the GH release. Somatostatin completely abolished the GH secretion mediated by theophyllin, but not that of PL. Some peculiarities in the formation of labeled GH and PL pool in the cells and secretion of these hormones into the culture medium are discussed.
D003994:Bucladesine / Q000494:pharmacology*; D002478:Cells, Cultured; D004347:Drug Interactions; D013006:Growth Hormone / Q000378:metabolism; D010903:Pituitary Gland, Anterior / Q000378:metabolism*; D011388:Prolactin / Q000378:metabolism; D013004:Somatostatin / Q000494:pharmacology*; D013806:Theophylline / Q000494:pharmacology*
D013004:Somatostatin; D003994:Bucladesine; D011388:Prolactin; D013006:Growth Hormone; D013806:Theophylline
Funktsional'naia aktivnost' kletok adenogipofiza krys v pervichnoĭ monosloĭnoĭ kul'ture i vliianie na nee nekotorykh reguliatorov.