[Several virologic and biochemical aspects of vaccination with antipoliomyelitis vaccine. Characteristics of intracellular virus-specific structures].
The data are presented concerning infectious potentials of subvirus particles isolated from cytoplasmic extracts of various organs of cotton rats and guinea pigs inoculated intramuscularly with purified and concentrated poliomyelitis virus type II. As described earlier, from various organs of the animals (the brain, spinal cord, lungs, and spleen) subvirus particles were isolated which in cesium chloride gradient had a density higher by 0.05-0.1 g/ml than that of the intact virus. Determinations of the infectious titre of these subparticles in susceptible cells showed their infectivity to be lower by 4-5 lg than that of the infact virus. When DEAE-dextran was used, a titre of both subvirus particles isolated from cytoplasmic extracts of various organs of the immunized animals and of the original poliomyelitis virus increased by 3-4 lg. The subvirus particles were sensitive to pancreatic RN-ase and to the antiserum against poliovirus type II. All the foregoing permits a conclusion that attenuated type II poliovirus or a portion of its population after inoculation into the living body is capable of long-term persistence there in the form of subviral particles with high buoyant density values.
D000818:Animals; D001921:Brain / Q000382:microbiology; D002499:Centrifugation, Density Gradient; D003911:Dextrans / Q000494:pharmacology; D006168:Guinea Pigs; D008168:Lung / Q000382:microbiology; D011051:Poliomyelitis / Q000382:microbiology* / Q000517:prevention & control*; D017955:Poliovirus / Q000187:drug effects / Q000302:isolation & purification* / Q000472:pathogenicity; D011054:Poliovirus Vaccine, Inactivated; D051381:Rats; D012260:Ribonucleases / Q000494:pharmacology; D013116:Spinal Cord / Q000382:microbiology; D013154:Spleen / Q000382:microbiology; D014611:Vaccination; D014613:Vaccines, Attenuated; D014774:Virulence
D003911:Dextrans; D011054:Poliovirus Vaccine, Inactivated; D014613:Vaccines, Attenuated; D012260:Ribonucleases
Izuchenie virusologicheskikh i biokhimicheskikh aspektov vaktsinatsii protivopoliomielitnoĭ vaktsinoĭ. Nekotorye kharakteristiki vnutrikletochnykh virusspetsificheskikh struktur.