A simple radioassay for angiotensin-converting enzyme.
Angiotensin-converting enzyme can be measured by the rate of release of 3H-labelled hippurate from p-[3H]benzoylglycylglycylglycine. The product is separable from the substrate by extraction of acidified reaction mixtures with ethyl acetate. Assay results for human serum angiotensin-converting enzyme can be obtained within 1.5 h of receipt of serum samples. Within the limits tested, the assay appears to be specific. However, interference by hitherto unrecognized enzymes of abnormal sera must be ruled out.
D006626:Hippurates; D009842:Oligopeptides; D007703:Peptidyl-Dipeptidase A / Q000032:analysis*; D014316:Tritium
D006626:Hippurates; D009842:Oligopeptides; D014316:Tritium; D007703:Peptidyl-Dipeptidase A