Expression of a bacterial gene turned on by a potent carcinogen.
Contrary to mutagenesis, lysogenic induction produced by chemical carcinogens occurs in the majority of a population of lysogenic cells. Such a mass effect can therefore be measured at the biochemical level using an E. coli tester strain in which the galactose operon has been put under the negative control of the lambda repressor. In this publication we show that galactokinase synthesis is turned on by aflatoxin B1 metabolites within an hour after treatment of the tester bacteria. Such a biochemical assay provides a useful means for identifying potential chemical carcinogens.
D000348:Aflatoxins / Q000494:pharmacology*; D002273:Carcinogens; D003090:Coliphages; D004353:Drug Evaluation, Preclinical; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D008242:Lysogeny / Q000187:drug effects*; D010770:Phosphotransferases / Q000378:metabolism*
D000348:Aflatoxins; D002273:Carcinogens; D010770:Phosphotransferases