Structure of a major glycophosphoceramide from tobacco leaves, PSL-I: 2-deoxy-2-acetamido-D-glucopyranosyl(alpha1 leads to 4)-D-glucuronopyranosyl(alpha1 leads to 2)myoinositol-1-O-phosphoceramide.
The chemical structure of a major glycophosphoceramide from tobacco leaves, called PSL-I [K. Kaul and R. L. Lester (1975), Plant Physiol. 55, 120], has now been characterized as 2-deoxy-2-acetamido-D-glucopyranosyl(alpha1 leads to 4)D-glucuronopyranosyl(alpha1 leads to 2)myoinositol-1-O-phosphoceramide. Sites of glycoside linkage were determined by (1) methylation analysis on a trisaccharide isolated by degradation of carboxyl-reduced PSL-I and (2) periodate oxidation experiments on PSL-I. The resulting products were identified with gas chromatography/mass spectrometry. Anomeric configurations were determined by resistance of the sugars in the peracetylated trisaccharide to chromium trioxide treatment.
D006028:Glycosphingolipids / Q000302:isolation & purification; D013058:Mass Spectrometry; D008745:Methylation; D008968:Molecular Conformation; D009844:Oligosaccharides / Q000032:analysis; D010084:Oxidation-Reduction; D010504:Periodic Acid; D010944:Plants / Q000032:analysis*; D010947:Plants, Toxic; D014026:Nicotiana / Q000032:analysis
D006028:Glycosphingolipids; D009844:Oligosaccharides; D010504:Periodic Acid