Detection of complement-dependent lytic antibodies in sera from feline leukemia virus-infected cats by the chromium-51 release assay.
Sera from feline leukemia virus-infected cats lysed FL74 cells in the presence of an appropriate complement source; lysis was detected with the use of the 51 Cr release method. Serum complement from both rabbits and guinea pigs mediated lysis. Partial correlation of results was obtained from preliminary comparisons of lysis and immunofluorescence tests with the use of FL74 cells as targets.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000918:Antibody Specificity; D000956:Antigens, Viral; D002415:Cats; D002455:Cell Division; D002470:Cell Survival; D002478:Cells, Cultured; D002860:Chromium Radioisotopes; D003168:Complement Fixation Tests; D003601:Cytotoxicity Tests, Immunologic; D005455:Fluorescent Antibody Technique; D066298:In Vitro Techniques; D007700:Kinetics; D005256:Leukemia Virus, Feline / Q000276:immunology*; D007942:Leukemia, Experimental / Q000276:immunology*; D013696:Temperature
D000914:Antibodies, Viral; D000956:Antigens, Viral; D002860:Chromium Radioisotopes