Enzyme histochemistry as a link between biochemistry and morphology.
The presented paper describes the role of enzyme histochemistry in cell biological investigations. In the first chapter a general discussion has been given about enzyme histochemistry as a connecting link between biochemistry and morphology. The methods available for determination of enzymes in a particular cell or cell compartment have been reviewed. In this respect the characteristics of enzyme histochemistry have been discussed. Furthermore, attention has been paid to the possibilities and limitations of enzyme histochemistry. In chapter two a comparison has been made between histochemically judged and biochemically determined enzyme activities. Some fundamental differences between the biochemical and the histochemical approach in cell biological investigations are dealt with. To correlate histochemically and biochemically determined enzyme activities, a description has been given of the application of histochemical methods on isolated fractions and sucrose-ficoll gradients of these fractions. Several experimental results are described concerning the question whether a relation exists between histochemically and biochemically determined activities of respectively alkaline phosphatase, glucose-6-phosphatase, 5'-nucleotidase and 3ss-hydroxysteroid dehydrogenase. From these results the conclusion could be drawn that in general a good correlation exists between histochemically judged activity per volume (area X thickness) and biochemically determined activity per gram tissue. In chapter three the role of enzymes as markers of cellular particles and as parameters of metabolic pathways is described. Histochemical methods are available for most marker enzymes. Only activities of key enzymes can be regarded as parameters of metabolic pathways. The distribution in sucrose-ficoll gradients of enzymes, regarded as markers of mitochondria, lysosomes, endoplasmic reticulum and plasma membranes has been given. The changes occur ing under different experimental conditions for a number of marker enzymes in rat liver are described. Attention has been given to the contibution of enzyme histochemistry in the study of the heterogeneity of mitochondria, the dual localization of some (lysosomal) enzymes, the complexity of the microsomal fraction, the function of the Golgi apparatus and the heterogeneity and function of plasma membranes. Based on these results and on literature findings the possible role of some marker enzymes in cell metabolism has been discussed. In chapter four problems coherent with species and sex differences in enzyme activities are described. The interpretation of histochemical and biochemical results in view of these differences is discussed. Enzymes characteristic for a given cell type -3ss-hydroxysteroid dehydrogenase in steroid producing cells, ATP-ase in liver plasma membrane surrounding the bile canaliculi - do show less variations between species and sexes than enzymes not directly involved in specialized functions...
D000251:Adenosine Triphosphatases / Q000032:analysis; D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D001667:Binding, Competitive; D002462:Cell Membrane / Q000201:enzymology; D004721:Endoplasmic Reticulum / Q000201:enzymology; D004798:Enzymes / Q000032:analysis*; D004950:Esterases / Q000032:analysis; D005952:Glucose-6-Phosphatase / Q000032:analysis; D006056:Golgi Apparatus / Q000201:enzymology; D006651:Histocytochemistry / Q000379:methods; D006913:Hydroxysteroid Dehydrogenases / Q000032:analysis; D007931:Leucyl Aminopeptidase / Q000032:analysis; D008247:Lysosomes / Q000201:enzymology; D008928:Mitochondria / Q000201:enzymology; D009246:NADH Tetrazolium Reductase / Q000032:analysis; D009708:Nucleotidases / Q000032:analysis; D009940:Organoids / Q000648:ultrastructure; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis; D012737:Sex Factors; D012995:Solubility; D013045:Species Specificity
D004798:Enzymes; D006913:Hydroxysteroid Dehydrogenases; D009246:NADH Tetrazolium Reductase; D004950:Esterases; D009708:Nucleotidases; D000469:Alkaline Phosphatase; D010744:Phosphoric Monoester Hydrolases; D005952:Glucose-6-Phosphatase; D007931:Leucyl Aminopeptidase; D000251:Adenosine Triphosphatases