title: Immunoglobulin response to bluetongue virus soluble antigen in subcutaneous chambers.
abstract: Group-specific antibodies were produced by inoculation of bluetongue virus soluble antigen into polyethylene chambers implanted subcutaneously in 8 rabbits and 2 sheep. For comparison, 5 rabbits and 1 sheep were inoculated intramuscularly with the soluble antigen in Freund's complete adjuvant. Antibodies present in the serum and chamber fluids were detected by the agar gel precipitin or serum-neutralization tests, qualitatively examined by immunoelectrophoresis and immunofluorescence, and quantitated by electroimmunodiffusion.
mesh_terms: D000818:Animals; D000917:Antibody Formation; D000956:Antigens, Viral; D001820:Bluetongue virus / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000032:analysis; D007158:Immunologic Techniques; D008722:Methods; D011817:Rabbits / Q000276:immunology; D012087:Reoviridae / Q000276:immunology*; D012756:Sheep / Q000276:immunology
chemical_list: D000956:Antigens, Viral; D007074:Immunoglobulin G