Absence of adenosine deaminase activity in a mammalian cell line transformed by Rous sarcoma virus.
No detectable adenosine deaminase activity was found in whole cells or 105,000g cytosol preparations of B-mix K-44/6 cells when either [3H]adenosine or [3H]arabinosyladenine was used as substrate. When grown in tissue culture medium supplemented with horse serum these cells provide a deaminase-free system not requiring the use of an adenosine deaminase inhibitor.
D000241:Adenosine / Q000378:metabolism; D000243:Adenosine Deaminase / Q000172:deficiency*; D001358:Avian Sarcoma Viruses; D002460:Cell Line; D002472:Cell Transformation, Viral; D009700:Nucleoside Deaminases / Q000172:deficiency*; D013379:Substrate Specificity; D014740:Vidarabine / Q000378:metabolism
D009700:Nucleoside Deaminases; D000243:Adenosine Deaminase; D014740:Vidarabine; D000241:Adenosine