title: Transformation of lymphocytes by Herpesvirus papio.
abstract: Cotton-topped (CT) or white-lipped (WL) marmoset lymphocytes were transformed in vitro with herpesvirus papio (HVP) into permanently growing lymphoblastoid cell lines (LCL). Five of 9 HVP-transformed CT cell lines contained cells with antigens reacting with antibodies to Epstein-Barr virus (EBV) capsid antigen (VCA) and/or to EBV-induced early antigens (EA). None of 12 WL LCL revealed such antigen-producing cells. Cells from both groups of cultures failed to react with antibodies to the EBV-specified nuclear antigen (EBNA). Exposure of baboon circulating lymphocytes to X-irradiated HVP or EBV-carring cells, or to suspensions of EBV resulted in establishment of LCL which all contained VCA and/or EA-positive, but no EBNA-positive cells. Nuclear antigens were undetectable also with anti-VCA-positive sera from baboons, chimpanzees, or other non-human primates. DNA-complementary RNA (cRNA) filter hybridization with EBV cRNA showed that with one exception transformed CT or WL marmoset cells contained at least 1-2 virus genome equivalents per cell, while at least 12-25 virus genome equivalents per cell were detected in transformed baboon cells. These data need confirmation by DNA-DNA reassociation kinetics.
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002143:Callitrichinae; D002460:Cell Line; D002875:Chromosomes; D004279:DNA, Viral / Q000032:analysis; D000882:Haplorhini; D006564:Herpesviridae / Q000276:immunology*; D004854:Herpesvirus 4, Human / Q000276:immunology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000648:ultrastructure; D009693:Nucleic Acid Hybridization; D010215:Papio; D018139:Simplexvirus / Q000276:immunology*
chemical_list: D000956:Antigens, Viral; D004279:DNA, Viral