title: Microwave fixation of brain tissue as a neurochemical technique- a review.
abstract: Microwave devices have been developed for rapidly inactivating brain enzymes by focusing the power output into the heads of small laboratory animals. The rapid inactivation achieved prevents postmortem changes and permits the measurement of neurochemicals such as acetylcholine at concentrations close to those obtained in vivo. The technique promises the assay of neurochemical parameters not possible before.
mesh_terms: D006916:5-Hydroxytryptophan / Q000032:analysis; D000109:Acetylcholine / Q000032:analysis; D000255:Adenosine Triphosphate / Q000032:analysis; D000818:Animals; D001921:Brain / Q000201:enzymology; D001923:Brain Chemistry / Q000528:radiation effects*; D002531:Cerebellum / Q000032:analysis; D002794:Choline / Q000032:analysis; D002795:Choline O-Acetyltransferase / Q000528:radiation effects; D002802:Cholinesterases / Q000528:radiation effects; D000242:Cyclic AMP / Q000032:analysis; D004298:Dopamine / Q000032:analysis; D005230:Fatty Acids, Nonesterified / Q000032:analysis; D005971:Glutamates / Q000032:analysis; D006652:Histological Techniques; D006897:Hydroxyindoleacetic Acid / Q000032:analysis; D051379:Mice; D008872:Microwaves; D009638:Norepinephrine / Q000032:analysis; D010725:Phosphocreatine / Q000032:analysis; D011180:Postmortem Changes; D051381:Rats; D012701:Serotonin / Q000032:analysis; D005680:gamma-Aminobutyric Acid / Q000032:analysis
chemical_list: D005230:Fatty Acids, Nonesterified; D005971:Glutamates; D010725:Phosphocreatine; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D005680:gamma-Aminobutyric Acid; D000255:Adenosine Triphosphate; D006916:5-Hydroxytryptophan; D000242:Cyclic AMP; D002795:Choline O-Acetyltransferase; D002802:Cholinesterases; D002794:Choline; D000109:Acetylcholine; D004298:Dopamine; D009638:Norepinephrine