Assay of bovine interferons in cultures of the porcine cell line IB-RS-2.
An assay for bovine interferons has been developed using the porcine cell line IB-RS-2 and a bovine enterovirus, CBV-D, as challenge virus. The method is based on estimation of cytopathic effect measured by uptake of neutral red. Teh assay is simple, sensitive, and reproducible. A comparative test of different viruses in IB-RS-2 cells and secondary calf kidney cells revealed that the sensitivity of a virus to interferon can vary up to 1,000-fold in the two cell systems. Vesicular stomatitis virus was found to be rather insensitive to interferon in IB-RS-2 cells.
D000818:Animals; D005537:Aphthovirus / Q000276:immunology; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D004770:Enterovirus / Q000276:immunology; D007372:Interferons / Q000032:analysis*; D008722:Methods; D013552:Swine; D014721:Vesicular stomatitis Indiana virus / Q000276:immunology
D007372:Interferons