Binding of 125I-labelled FSH in the mouse testis in vitro.
In-vivo testicular binding of highly purified pituitary FSH, labelled by a method which did not significantly affect biological potency, was hormone-specific, tissue-specific and dose-dependent. Hypophysectomy of mice was followed by a progressive increase in the amount of 125I-labelled FSH per unit weight of testis but not in the total amount of hormone taken up by the testis. Maximum binding occurred at 4 h in a membrane-containing fraction prepared by high-speed ultracentrifugation of testicular homogenate. This is considerably later than has been reported for testicular tissue incubated in vitro at 37 degrees C.
D000818:Animals; D005640:Follicle Stimulating Hormone / Q000378:metabolism*; D007016:Hypophysectomy; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D011956:Receptors, Cell Surface / Q000378:metabolism*; D013737:Testis / Q000378:metabolism*
D011956:Receptors, Cell Surface; D005640:Follicle Stimulating Hormone