[Sulfhydryl groups of cyclic AMP-dependent histone kinase from pig brain].
A preparative method of isolation of the histone kinase regulatory subunit has been developed. The total number of cysteine residues in the enzyme (13 residues) and in each of its subunits (3 residues in the catalytic subunit and 10 residues in the regulatory one), has been determined. Two disulfide bridges have been found in the regulatory subunits. The relative availability of the sulfhydryl groups with respect to different modifying agents has been studied. It has been demonstrated that SH-groups blocking does not change essentially the enzyme activity.
D000596:Amino Acids / Q000302:isolation & purification; D000818:Animals; D001921:Brain / Q000201:enzymology*; D002848:Chromatography, DEAE-Cellulose; D000242:Cyclic AMP; D006657:Histones; D006656:Protamine Kinase / Q000032:analysis*; D011494:Protein Kinases / Q000032:analysis*; D011953:Receptors, Cyclic AMP; D013438:Sulfhydryl Compounds / Q000302:isolation & purification*; D013552:Swine
D000596:Amino Acids; D006657:Histones; D011953:Receptors, Cyclic AMP; D013438:Sulfhydryl Compounds; D000242:Cyclic AMP; D011494:Protein Kinases; D006656:Protamine Kinase
Sul'fgidril'nye gruppy tdiklo-AMP-zavisimoĭ gistonkinazy iz mozga svin'i.