An assessment of the effects of enzyme inducers on aryl hydrocarbon hydroxylase activity.
The aryl hydrocarbon hydroxylase activity for control, Aroclor 1254 and FireMaster BP-6 induced hepatic microsomes was determined using 4-chlorobiphenyl, a model PCB isomer, as the substrate. The assay system was used to quantitate the amount of metabolites, conjugates and macromolecular adducts formed for the induced and control microsomes. The metabolites and conjugates were designated detoxification (D) products and the macromolecular adducts designated as toxification (T) products. From the data it was then possible to determine a T/D index for each inducer and it is proposed that this index is useful in assessing the potential toxicity of microsomal enzyme inducers.
D000818:Animals; D001140:Aroclors / Q000494:pharmacology; D001189:Aryl Hydrocarbon Hydroxylases / Q000378:metabolism*; D004790:Enzyme Induction / Q000187:drug effects*; D066298:In Vitro Techniques; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology; D011075:Polybrominated Biphenyls / Q000494:pharmacology; D051381:Rats
D001140:Aroclors; D011075:Polybrominated Biphenyls; D001189:Aryl Hydrocarbon Hydroxylases