title: A portion of the feline leukaemia virus genome is not endogenous in cat cells.
abstract: Although viral sequences closely related to feline leukaemia virus are represented in multiple copies in cellular DNA of all domestic cats, a specific fraction was present only in the virus-infected cells. This fraction was detected by viral cDNA enriched by a prior absorption of a total complementary DNA (cDNA) transcript with normal cat liver DNA. The recycled cDNA hybridized well with the cellular DNA of virus-infected cells, but to a lesser extent with DNA from uninfected cat cells. The probe was used to differentiate virus-positive from virus-negative tumour tissues of cats. The same approach with cDNA of another endogenous feline virus, RD114, failed to show any difference between a virus-infected cell line and normal cells, including both virus-inducible and non-inducible lines.
mesh_terms: D000818:Animals; D002415:Cats; D002460:Cell Line; D004247:DNA; D004273:DNA, Neoplasm; D004279:DNA, Viral; D005814:Genes, Viral; D005256:Leukemia Virus, Feline; D007942:Leukemia, Experimental; D009693:Nucleic Acid Hybridization; D014158:Transcription, Genetic
chemical_list: D004273:DNA, Neoplasm; D004279:DNA, Viral; D004247:DNA