Surface glycoproteins and concanavalin-A-mediated agglutinability of clonal variants and tumour cells derived from SV40-virus-transformed mouse 3T3 cells.
Cell strains isolated from an established line of SV40-transformed mouse 3T3 cells (SV3T3) showed large variations in the various parameters of transformation, viz. saturation density, serum requirement, contact inhibition of movement and of growth and Concanavalin-A-mediated agglutinability. These cell strains were studied for changes in elution profiles of fucose-labelled surface glycoproteins, using actively growing, untransformed 3T3 cells as controls. A cell strain established from a tumour arising after injection of wild-type SV3T3 cells and SV3T3 cells grown in vivo in diffusion chambers, was similarly studied. Changes in surface glycoproteins were observed only in the tumour-derived cell strain. The results suggest that alterations in surface glycoproteins are associated with the tumorigenic potential of the cells rather than with the transformed phenotype per se.
D000371:Agglutination; D000818:Animals; D002449:Cell Aggregation / Q000187:drug effects*; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D002999:Clone Cells; D003208:Concanavalin A / Q000494:pharmacology*; D004247:DNA / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis / Q000276:immunology*; D051379:Mice; D013539:Simian virus 40
D006023:Glycoproteins; D003208:Concanavalin A; D004247:DNA