Preparation of plasma membranes from line 10 and line 1 guinea pig hepatomas.
Gentle homogenization followed by differential and density gradient centrifugation was used to purify line 10 and line 1 guinea pig hepatoma plasma membranes in the form of ghosts. Yields of 15--25% allowed enough membranes to be obtained from a single ascites tumor-bearing animal for immunologic and biochemical studies. Although the plasma membrane marker enzyme (Na+ + k+)atpase was present in normal concentrations in both line 10 and line 1 hepatomas, 5'-nucleotidase was reduced over 100-fold in both tumors and phosphodiesterase I was increased 210-fold in the line 10 hepatomas.
D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D002462:Cell Membrane / Q000201:enzymology / Q000648:ultrastructure*; D005260:Female; D006168:Guinea Pigs; D008114:Liver Neoplasms, Experimental / Q000201:enzymology / Q000648:ultrastructure*; D009708:Nucleotidases / Q000378:metabolism; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
D009708:Nucleotidases; D010727:Phosphoric Diester Hydrolases; D000254:Sodium-Potassium-Exchanging ATPase