Role of phospholipid in adrenaline-induced lipolysis and cyclic AMP production.
Lipid micelles consisting of a glyceride mixture (triolein, diolein, and monoolein) and lecithin bound adrenaline-14C more strongly than did micelles consisting of the glyceride mixture only. Lipid micelles consisting of the glyceride mixture and phosphatidic acid also bound adrenaline-14C effectivily, whereas lipid micelles consisting of the glyceride mixture and diglyceride, obtained from lecithin, did not bind the hormone strongly. Both phenoxybenzamine (an alpha- blocker) and propranolol (a beta-blocker) strongly inhibited the association between adrenaline-14C and lipid micelles consisting of the glyceride mixture and lecithin. Propranolol, inhibited adrenaline-induced lipolysis in both fat cells and fat globules, whereas, phenoxybenzamine, did not affect adrenaline-induced lipolysis. Both agents reduced adrenaline-induced adenyl-cyclase activation in fat cell ghosts. Phospholipid was also found to be related with adrenaline-mediated adenylcyclase activation.
D000262:Adenylyl Cyclases / Q000378:metabolism; D000273:Adipose Tissue / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D000242:Cyclic AMP / Q000096:biosynthesis*; D004837:Epinephrine / Q000494:pharmacology*; D005989:Glycerides; D008053:Lipid Mobilization / Q000187:drug effects*; D008297:Male; D008567:Membranes, Artificial; D010743:Phospholipids / Q000502:physiology*; D051381:Rats; D013565:Sympatholytics / Q000494:pharmacology
D005989:Glycerides; D008567:Membranes, Artificial; D010743:Phospholipids; D013565:Sympatholytics; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D004837:Epinephrine