title: [Interaction of thiamine pyrophosphokinase from brewer's yeast with thiamine and adenosine-5'-triphosphate].
abstract: The interaction of thiamine pyrophosphokinase (Thiaminkinase EC 2.7.6.2) with thiamine and ATP was studied. It was shown that the mechanism of the thiaminokinase reactions is Rapid Equilibrium Random Bi -- Bi. The enzyme binds 8 moles ATP and 1 mole thiamine per mole protein Ks = 0,8-10(-3) and 4.10(-6) M for ATP and thiamine respectively.
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D007700:Kinetics; D010770:Phosphotransferases / Q000378:metabolism*; D011485:Protein Binding; D013831:Thiamine / Q000378:metabolism*
chemical_list: D000255:Adenosine Triphosphate; D010770:Phosphotransferases; D013831:Thiamine
vernacular_title: Vzaimodeĭstvie tiamina i adenozin-5'-trifosfata sdrozhzhevoĭ tiaminpirofosfokinazoĭ