Glucose transport and metabolism in cultured cells of nervous tissue.
The effect of the concentration of glucose in the medium on the intracellular concentrations of metabolites of C-6 astrocytoma cells and C-1300 neuroblastoma cells in culture has been investigated. The intracellular concentrations of glucose, glycogen, glucose 6-P and UDP-glucose were measured at intervals after feeding the cells. A rapid increase in glucose and glucose 6-P levels occurred when fresh medium containing 5.5 mM glucose was applied to the cells, followed by slower increases in UDP-glucose andglycogen. When the medium glucose was increased ten-fold, the intracellular concentration of glucose was increased, but the level of glucose 6-P, UDP=-glucose and glycogen were not altered, nor were the rates of accumulation. The addition of insulin to the medium resulted in an increase of intracellular glucose, glucose 6-P and glycogen. The transport of glucose into the cells is not the rate-limiting step of the regulation of metabolite levels in the cells.
D001254:Astrocytoma; D001693:Biological Transport, Active; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D005958:Glucosephosphates / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology; D009447:Neuroblastoma; D009474:Neurons / Q000378:metabolism; D014532:Uridine Diphosphate Glucose / Q000378:metabolism
D005958:Glucosephosphates; D007328:Insulin; D006003:Glycogen; D000242:Cyclic AMP; D005947:Glucose; D014532:Uridine Diphosphate Glucose