Cyclic AMP response to prostaglandin E1 in mononuclear cells from peripheral blood and synovial fluid of patients with rheumatoid arthritis.
The cyclic AMP response to prostaglandin E1 (PGE1) was studied in peripheral blood (PB) and synovial fluid (SF) mononuclear cells from patients with rheumatoid arthritis (RA). The PGE1 induced accumulation of cyclic AMP was consistently (7 of 8 patients) less in cell suspensions derived from SF than in suspensions of equivalent numbers of mononuclear cells obtained simultaneously from PB. The high PB/SF cyclic AMP ratio was seen most clearly at the lowest concentration (10(-6)M) of PGE1 tested. There was no correlation between the patients' therapy and cyclic AMP response to PGE1. The high PB/SF cyclic AMP ratio was not accounted for by the presence of platelets in PB cell suspensions.
D001172:Arthritis, Rheumatoid / Q000378:metabolism*; D001792:Blood Platelets / Q000378:metabolism; D002469:Cell Separation; D000242:Cyclic AMP / Q000097:blood / Q000378:metabolism*; D006801:Humans; D009000:Monocytes / Q000378:metabolism; D011458:Prostaglandins E / Q000494:pharmacology*; D013582:Synovial Fluid / Q000378:metabolism*
D011458:Prostaglandins E; D000242:Cyclic AMP