Increased polyethylene glycol-mediated fusion competence in mitotic cells of a mouse lymphoid cell line.
Spontaneous mitotic cells of the mouse leukemic cell line GF7 are preferentially included in cell fusion products after treatment with polyethylene glycol (PEG). This implies a unique configuration of the natural mitotic membrane which is particularly vulnerable to induction of fusion by PEG. Colcemid-arrested GF7 mitotic cells, however, are excluded from PEG-induced cell fusion products, suggesting that colcemid reverses the membrane configuration which is susceptible to the action of PEG. When Sendai virus is used as the fusogenic agent, both colcemid-arrested and spontaneous mitotic cells are selectively fused. There must, therefore, be an essential membrane-fusogen reaction which is characteristically different for each of these agents.
D002459:Cell Fusion / Q000187:drug effects*; D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology*; D003703:Demecolcine / Q000494:pharmacology; D007938:Leukemia; D008213:Lymphocyte Activation; D008938:Mitosis; D010222:Parainfluenza Virus 1, Human; D011092:Polyethylene Glycols / Q000494:pharmacology*; D013936:Thymidine / Q000378:metabolism
D011092:Polyethylene Glycols; D013936:Thymidine; D003703:Demecolcine