Application of freeze-dried, one-day-old chick erythrocytes to viral hemagglutination and hemagglutination-inhibition tests.
Erythrocytes collected from 1-day-old chicks were stabilized by fixation with formaldehyde and by freeze-drying after treatment with carbon monoxide. Suspensions of freeze-dried erythrocytes in distilled water or physiological saline had a homogeneous bright reddish-purple color. Freeze-dried erythrocytes were compared with fresh erythrocytes for hemagglutination and hemagglutination-inhibition tests for various viruses including rubella, Japanese encephalitis, influenza, mumps, Newcastle disease, and Sendai viruses. After storage at 4 degrees C for 1 year or more, freeze-dried erythrocytes maintained their original appearance and sensitivity to hemagglutination antigens.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D002645:Chickens / Q000276:immunology; D004664:Encephalitis Virus, Japanese / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology*; D005612:Freeze Drying; D000882:Haplorhini; D006385:Hemagglutination Inhibition Tests; D006386:Hemagglutination Tests; D051379:Mice; D009109:Mumps virus / Q000276:immunology; D009522:Newcastle disease virus / Q000276:immunology; D009975:Orthomyxoviridae / Q000276:immunology; D010222:Parainfluenza Virus 1, Human / Q000276:immunology; D011817:Rabbits; D051381:Rats; D012412:Rubella virus / Q000276:immunology
D000914:Antibodies, Viral