[Electron-cytochemical study of nervous tissue at different periods after death].
Using electron cytochemistry the authors studied the brain frontal cortex and hypothalamus in rats and humans in different periods following death. With the aid of 3,3'-diaminobenzidin, cytochrome C which was located inside the crystal and between the external and internal membrane of the mitochondria was detected. ATP-ase and acid phosphatase was revealed by the lead method. The activity of acid phosphotase was demonstrated in the nucleoli, along the membrane of the cytoplasmatical reticulum, in the symaptical terminals and in the circulatory capillaries. It was established that in the human brain it is possible to study the localization of the cytochrome C up to 24 hours, ATP-ase up to 15-20 hours and acid phosphatase up to 12 hours following death.
D000135:Acid Phosphatase / Q000378:metabolism; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D003574:Cytochrome c Group / Q000378:metabolism; D005625:Frontal Lobe / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure; D006651:Histocytochemistry; D006801:Humans; D007031:Hypothalamus / Q000378:metabolism*; D008062:Lipofuscin / Q000378:metabolism; D008247:Lysosomes / Q000378:metabolism / Q000648:ultrastructure; D008854:Microscopy, Electron; D008928:Mitochondria / Q000378:metabolism / Q000648:ultrastructure; D011180:Postmortem Changes; D051381:Rats; D013495:Supraoptic Nucleus / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure; D013997:Time Factors
D003574:Cytochrome c Group; D008062:Lipofuscin; D000135:Acid Phosphatase; D000251:Adenosine Triphosphatases
Elektronno-tsitokhimicheskoe issledovanie nervnoĭ tkani v raznye sroki posle smerti.