[Superinduction of interferon and and a study of its messenger RNA].
Combined use of interferon inductor poly-IC and antibiotics (cycloheximide and actinomycin D) provided a significant increase (up to 1000 times) in interferon production by chick, mouse, monkey and human cells. Messenger RNA with matrix activity for interferon (mRNA-IF) was isolated from superinduced cells. On translation of mRNA-IF in homogenous and heterogenous cells the specificity of interferons produced was determined by the type of the cells from which mRNA-IF was isolated. Sedimentation analysis of various mRNA-IF revealed 2 peaks of activity: major (5--15S) and minor (25--30S).
D000818:Animals; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D002645:Chickens; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004666:Encephalitis Virus, Venezuelan Equine; D000882:Haplorhini; D006801:Humans; D007369:Interferon Inducers / Q000494:pharmacology*; D007372:Interferons / Q000032:analysis / Q000096:biosynthesis*; D051379:Mice; D011070:Poly I-C / Q000494:pharmacology; D014176:Protein Biosynthesis / Q000187:drug effects; D012333:RNA, Messenger / Q000032:analysis / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects; D014721:Vesicular stomatitis Indiana virus
D007369:Interferon Inducers; D012333:RNA, Messenger; D003609:Dactinomycin; D007372:Interferons; D003513:Cycloheximide; D011070:Poly I-C
Superinduktsiia interferona i izuchenie ego informatsionnykh