Transplacental transmission of polyoma virus in mice.
When pregnant mice were inoculated on day 1 of gestation with polyoma, some of them exhibited total resorption or reduced litter size, the extent depending on the dose of virus. Virus was detected in 4 out of 11 mouse embryo fibroblast (MEF) cultures made from infected mothers. After maternal infection on day 5 or 10 of gestation, virus titers of up to 10(7) 50% tissue culture infectious doses (TCID50)/g of fetus were found in all pools of fetuses tested 5 days later, with the titers falling by day 6. Hemagglutination-inhibiting antibodies against polyoma appeared in maternal serum by day 6 and rose to a maximum by day 14. Immunoglobulin G class antibodies were detected by day 7, with titers rising rapidly to a maximum at day 14. After maternal infection later in gestation (day 15), one out of three litters of newborn mice was found to have 10(5) TCID50 polyoma virus per g in pooled kidney samples.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D001769:Blood / Q000382:microbiology; D005260:Female; D005327:Fetal Resorption / Q000209:etiology; D005333:Fetus / Q000382:microbiology; D005455:Fluorescent Antibody Technique; D005865:Gestational Age; D006389:Hemagglutinins, Viral / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D008431:Maternal-Fetal Exchange; D051379:Mice; D010053:Ovary / Q000382:microbiology; D011120:Polyomavirus / Q000254:growth & development* / Q000276:immunology; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000276:immunology / Q000382:microbiology*; D014412:Tumor Virus Infections / Q000150:complications / Q000382:microbiology*
D000956:Antigens, Viral; D006389:Hemagglutinins, Viral; D007074:Immunoglobulin G