Mitochondrial biogenesis in cultured animal cells. I. Effect of chloramphenicol on morphology and mitochondrial respiratory enzymes.
The effects of chloramphenicol on the morphology and respiratory enzymes of BHK-21 cells in spinner culture have been examined with time. Cells treated with chloramphenicol double twice before growth ceases; these cells have increased size as measured by several techniques. Mitochondria are enlarged and appear to degenerate with prolonged treatment. Cytochrome c oxidase and succinate cytochrome c reductase activities are reduced while there is no decrease in the activities of monoamine oxidase, glutamate dehydrogenase or NADPH-cytochrome c reductase. Cytochromes aa3 and b disappear on treatment while cytochromes c + c1 appears to be unaffected. All these effects are reversible if chloramphenicol is removed within a limited period of time.
D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002701:Chloramphenicol / Q000494:pharmacology*; D003579:Cytochrome Reductases / Q000378:metabolism*; D003574:Cytochrome c Group / Q000378:metabolism; D003580:Cytochromes / Q000378:metabolism*; D003576:Electron Transport Complex IV / Q000378:metabolism*; D005969:Glutamate Dehydrogenase / Q000378:metabolism*; D007700:Kinetics; D008854:Microscopy, Electron; D008928:Mitochondria / Q000187:drug effects / Q000201:enzymology / Q000502:physiology*; D008995:Monoamine Oxidase / Q000378:metabolism*; D010743:Phospholipids / Q000096:biosynthesis; D014176:Protein Biosynthesis; D013696:Temperature
D003574:Cytochrome c Group; D003580:Cytochromes; D010743:Phospholipids; D002701:Chloramphenicol; D005969:Glutamate Dehydrogenase; D008995:Monoamine Oxidase; D003579:Cytochrome Reductases; D003576:Electron Transport Complex IV