[Blood protein synthesis in isolated liver cells].
Cells obtained by continuous perfusion with collagenase maintained their ultrastructure and their capacity for protein synthesis in contrast to mechanically isolated rat liver cells. Albumin and angiotensinogen (renin substrate) are synthesized and secreted and the synthesis of both proteins is stimulated by addition of hydrocortisone. As cell suspensions allow the simultaneous investigation of several samples under well defined conditions they can serve as an excellent model for the study of regulatory mechanisms of serum protein synthesis.
D000596:Amino Acids / Q000378:metabolism / Q000627:therapeutic use; D000808:Angiotensinogen / Q000096:biosynthesis; D001798:Blood Proteins / Q000096:biosynthesis*; D002469:Cell Separation; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D003012:Microbial Collagenase; D011488:Protein Deficiency / Q000188:drug therapy; D012709:Serum Albumin / Q000096:biosynthesis
D000596:Amino Acids; D001798:Blood Proteins; D012709:Serum Albumin; D000808:Angiotensinogen; D003012:Microbial Collagenase; D006854:Hydrocortisone
Serumprotein-Synthese in isolierten Leberzellen