Cholesterol determination in high-density lipoproteins separated by three different methods.
We describe a simplified method for measuring high-density lipoprotein cholesterol in serum after very-low- and low-density lipoproteins have been precipitated from the specimen with sodium phosphotungstate and Mg2+. Values so obtained correlate well with values obtained with the heparin-Mn2+ precipitation technique (r = 0.95, CV less than 5% in 66% of the subjects studied and between 5 and 10% in the remaining ones) or by ultracentrifugal separation (r = 0.82, CV less than 5% in 80% of the subjects studied and between 5 and 10% in the remaining ones). Our precipitation technique is more appropriate for routine clinical laboratory use.
D000328:Adult; D011232:Chemical Precipitation; D002784:Cholesterol / Q000097:blood*; D006493:Heparin; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood* / Q000302:isolation & purification; D008274:Magnesium; D008345:Manganese; D008722:Methods; D010772:Phosphotungstic Acid; D014280:Triglycerides / Q000097:blood; D014461:Ultracentrifugation
D008075:Lipoproteins, HDL; D014280:Triglycerides; D010772:Phosphotungstic Acid; D008345:Manganese; D006493:Heparin; D002784:Cholesterol; D008274:Magnesium