Pathogenesis of infection with Bordetella pertussis in hamster tracheal organ culture.
Hamster tracheal organ culture was employed as a model for study of the pathogenesis of infection due to Bordetella pertussis. Infected tracheal explants were examined with light, immunofluorescence, and electrom microscopy. B. pertussis organisms preferentially attached to the ciliated cells, producing ciliostasis and marked destruction of the subcellular organelles followed by expulsion of these cells from the epithelial layer. Other nonciliated respiratory epithelial cells appeared to be unaffected. Metabolic studies on infected tracheal cultrues indicated that significant deficiencies in syntheiss of host cell protein accompanied early cytopathology. Similarities and differences in host cell and parasite interaction were noted between B. pertussis and other pathogenic agents studied in this system.
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001886:Bordetella pertussis; D006224:Cricetinae; D004847:Epithelial Cells; D004848:Epithelium / Q000473:pathology; D005455:Fluorescent Antibody Technique; D009924:Organ Culture Techniques; D014176:Protein Biosynthesis; D014132:Trachea / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology; D014917:Whooping Cough / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology
D000596:Amino Acids