[Quantitative amino acid makeup and the characteristics of the amino groups of ristomycin and the products of its partial acid hydrolysis].
The quantitative amino acid composition of ristomycin A, a glycopeptide antibiotic, peptides I-IV (from partial acid hydrolysis of the antibiotic) and their dinitrophenylic derivatives was determined. It was shown that both ristomycin and free peptides I-IV contained one residue of ristomycinic acid and one residue of actinoidinic acid, diamino-dicarbonic amino acids of the glycylphenolic type. Peptides I-IV had close molecular weights, i.e. 1100-1200 and differed from each other in the gradually increasing numbers of NH2- and COON- groups, from one in peptide I to four in peptide IV. The quantitative amino acid analysis of the dinitrophenylic derivatives of ristomycin and peptides I-IV showed that the free NH2-group in peptide I belonged to ristomycinic acid, the same as in the antibiotic, while in peptides III-IV at least one of the free NH2-groups belonged to ristomycinic acid and the other belonged to actinoidinic acid.
D000596:Amino Acids / Q000032:analysis*; D000900:Anti-Bacterial Agents / Q000032:analysis; D002845:Chromatography; D004140:Dinitrophenols / Q000032:analysis; D005609:Free Radicals; D006020:Glycopeptides / Q000032:analysis; D006868:Hydrolysis; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis; D012310:Ristocetin / Q000031:analogs & derivatives / Q000032:analysis*
D000596:Amino Acids; D000900:Anti-Bacterial Agents; D004140:Dinitrophenols; D005609:Free Radicals; D006020:Glycopeptides; D010455:Peptides; D012310:Ristocetin
Kolichestvennyĭ aminokislotnyĭ sostav i kharakteristika aminogrupp ristomitsina i produktov ego chastichnogo kislotnogo gidroliza.