Rifampin-susceptible mutant of vesicular stomatitis virus: protein and RNA synthesis.
A rifampin-susceptible strain (VSV Rif+) was selected from the wild vesicular stomatitis virus (VSV) population unsusceptible to rifampin. The VSV Rif+ was blocked in its intracellular replication in the presence of rifampin. In cells, rifampin affected primarily VSV Rif+ transcription, but to a different extent than in a cell-free system. In addition, a decrease in the amount of VSV Rif+ protein M was detected, linked to a stimulation of protein NS. In the absence of rifampin, protein M, although synthesized, was not immediately incorporated into the cell membrane. An interpretation of these observations is proposed.
D002474:Cell-Free System; D004352:Drug Resistance, Microbial; D009154:Mutation; D012333:RNA, Messenger / Q000096:biosynthesis*; D012367:RNA, Viral / Q000096:biosynthesis*; D012293:Rifampin / Q000494:pharmacology; D014158:Transcription, Genetic; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects / Q000378:metabolism*; D014764:Viral Proteins / Q000096:biosynthesis*
D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; D012293:Rifampin