Purineless death: ribosomal RNA turnover in a purine-starved ade- mutant of Chinese hamster cells.
When V79 pur 1, a purine-requiring auxotroph of a Chinese hamster cell line, is deprived of adenine, nucleic acid and protein synthesis decline rapidly. However, on continuous starvation RNA and DNA synthesis recommences to reach approximately 30% of the normal level between 12 to 24 h starvation. This is accompanied by a rise in the intracellular nucleotide pool. Utilizing mengovirus, which gives a productive infection in V79 pur 1 cells even under conditions of starvation, we can show that rRNA is preferentially degraded and provides the nucleotides for RNA synthesis. Thus "purineless" death in mammalian cells is accompanied by turnover of stable RNA.
D000225:Adenine / Q000378:metabolism* / Q000494:pharmacology; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002471:Cell Transformation, Neoplastic; D007700:Kinetics; D008574:Mengovirus; D009154:Mutation; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis; D012335:RNA, Ribosomal / Q000096:biosynthesis / Q000378:metabolism*; D012343:RNA, Transfer / Q000096:biosynthesis; D014529:Uridine / Q000378:metabolism
D012335:RNA, Ribosomal; D012313:RNA; D012343:RNA, Transfer; D000225:Adenine; D014529:Uridine