Regulation of sugar transport in chick embryo fibroblasts and in fibroblasts transformed by a temperature-sensitive mutant of the Rous sarcoma virus.
The mode of induction of sugar transport by serum-stimulation of growth and hexose-starvation in chick embryo fibroblasts (CEF) has been studied using metabolic inhibitors. We have concluded from these studies that the sugar transport increases induced by serum-stimulation are regulated by post-transcriptional mechanisms while sugar transport increases induced by hexose-starvation are regulated by a transcriptional mechanism. CEF infected with a temperature-sensitive mutant of the Rous sarcoma virus. Ts68 and incubated at the nonpermissive temperature for transformation, 41 degrees, retain the capacity to regulate sugar transport in a manner similar to uninfected CEF. However, Ts68-infected CEF maintained at the permissive temperature for transformation, 37 degrees, have lost the ability to regulate sugar transport at the post-transcriptional and post-translational levels.
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000254:growth & development; D001693:Biological Transport, Active; D001769:Blood; D002166:Camptothecin / Q000494:pharmacology; D050260:Carbohydrate Metabolism; D002471:Cell Transformation, Neoplastic; D002642:Chick Embryo; D003470:Culture Media; D046508:Culture Techniques; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D003839:Deoxyadenosines / Q000494:pharmacology; D005347:Fibroblasts / Q000378:metabolism*; D006601:Hexoses / Q000378:metabolism; D009154:Mutation; D013696:Temperature
D003470:Culture Media; D003839:Deoxyadenosines; D006601:Hexoses; D003609:Dactinomycin; D003513:Cycloheximide; D002166:Camptothecin