Expression of feline type-C virus in normal and tumor tissues of the domestic cat.
Tumor and normal tissues from domestic cats were examined for FeLV and RD-114 specific nucleic acids and proteins. Virus-positive tissues showed a 100- to 200-fold higher level of FeLV and a 200- to 400-fold higher level of FeLV p30 than virus-negative tumors and normal cat tissues. In contrast, the levels of RD-114 viral RNA and p30 antigen were comparable in the majority of tumorous and normal tissues examined. When the DNA of these tissues was examined for FeLV-related sequences, the virus-positive tumors were found to contain extra copies of FeLV DNA. Virus-negative tumors contained DNA sequences homologous with FeLV but the copy number of these sequences could not be distinguished from normal tissues. The results indicate that in certain FeLV-induced type-C lymphoproliferative diseases, extra type-C sequences not present in normal tissues can be identified.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002415:Cats; D002460:Cell Line; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D005256:Leukemia Virus, Feline / Q000276:immunology / Q000302:isolation & purification; D007942:Leukemia, Experimental / Q000276:immunology / Q000382:microbiology*; D009374:Neoplasms, Experimental / Q000276:immunology / Q000382:microbiology; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000032:analysis; D012208:Rhabdomyosarcoma / Q000276:immunology / Q000382:microbiology*; D013154:Spleen / Q000382:microbiology; D013696:Temperature; D013950:Thymus Gland / Q000382:microbiology; D014599:Uterus / Q000382:microbiology; D014764:Viral Proteins / Q000032:analysis
D000956:Antigens, Viral; D004279:DNA, Viral; D012367:RNA, Viral; D014764:Viral Proteins