Multiple interactions between murine cytomegalovirus and lymphoid cells in vitro.
Spleen cultures from various strains of mice were infected in vitro with murine cytomegalovirus (MCMV). Infectious centres were established in a small proportion (not greater than 1%) of the cells. Virus could be rescued from these cells by co-cultivation with syngeneic or allogeneic fibroblasts, but the frequency of rescue could not be altered by incubation with cyclic nucleotide analogues, iododeoxyuridine, cortisol, or allogeneic spleen cells. In addition a smaller fraction of the cell population, possibly a sub-population of the infectious centres, replicated virus spontaneously. The presence of mitogens did not affect these interactions qualitatively or quantitatively. A third response to infection was an inhibition in DNA synthesis, which was suffered by unstimulated cultures and by cells transformed by concanavalin A and bacterial lipopolysaccharides, although overall cell viability was maintained. This response was also mediated by u.v.-inactivated virus.
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D003208:Concanavalin A / Q000494:pharmacology; D046508:Culture Techniques; D003587:Cytomegalovirus / Q000254:growth & development*; D003588:Cytopathogenic Effect, Viral; D004247:DNA / Q000096:biosynthesis; D007065:Idoxuridine / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008815:Mice, Inbred Strains; D008934:Mitogens; D011135:Polysaccharides, Bacterial / Q000494:pharmacology; D013154:Spleen; D014779:Virus Replication
D008070:Lipopolysaccharides; D008934:Mitogens; D011135:Polysaccharides, Bacterial; D003208:Concanavalin A; D003994:Bucladesine; D004247:DNA; D007065:Idoxuridine