In vitro primary induction of cytotoxic T cells against virus-infected syngeneic cells.
An in vitro method is described for primary induction of murine cytotoxic T cells against syngeneic cells infected with ectromelia or lymphocytic choriomeningitis (LCM) virus. Cytotoxicity was assayed by 51Cr release from macrophage or L929 target cells. Cytotoxic activity was sensitive to anti-theta and complement and was expressed only against target cells infected with the same virus and sharing H-2K or H-2D genes with the infected stimulator cells. The crucial factors in generating responses were mouse strain, responder: stimulator ratio, nature of infected stimulator cells, and presence of sufficient macrophages. C57BL/6 cells were less demanding than CBA/H and BALB/c cells. Under optimal conditions defined here, the in vitro response had similar kinetics and potency to the primary response in the spleen in vivo.
D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D003601:Cytotoxicity Tests, Immunologic; D004306:Dose-Response Relationship, Immunologic; D004481:Ectromelia virus / Q000276:immunology; D004482:Ectromelia, Infectious / Q000276:immunology*; D006648:Histocompatibility; D007700:Kinetics; D008216:Lymphocytic Choriomeningitis / Q000276:immunology*; D008217:Lymphocytic choriomeningitis virus / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D011213:Poxviridae Infections / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D003470:Culture Media