Scanning electron, light, and immunofluorescent microscopy of coronaviral enteritis of turkeys (Bluecomb).
Intestinal sections from both experimental and field cases of turkey coronaviral enteritis (TCE) were examined by scanning electron microscopy and light microscopy through 10 days after inoculation and by a direct fluorescent antibody test for TCE through 12 days. Serums were collected for an indirect fluorescent antibody test for TCE through 160 days after inoculation. Lesions observed with the scanning electron microscopy were catarrhal enteritis with hemorrhage per diapedesis, epithelial desquamation, and villous atrophy which developed and regressed within 6 days after inoculation. Light microscopy demonstrated similar lesions, except that villus-to-crypt ratios remained depressed 10 days. The direct fluorescent antibody test demonstrated the presence of coronaviral antigen throughout the sampling period, and serum antibodies to TCE were present until at least 160 days, when the experiment was terminated.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D003332:Coronaviridae / Q000276:immunology; D003333:Coronaviridae Infections / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D004751:Enteritis / Q000276:immunology / Q000473:pathology / Q000662:veterinary*; D004753:Enteritis, Transmissible, of Turkeys / Q000473:pathology; D005455:Fluorescent Antibody Technique; D007422:Intestines / Q000276:immunology / Q000648:ultrastructure; D011201:Poultry Diseases / Q000276:immunology / Q000473:pathology*; D014422:Turkeys
D000956:Antigens, Viral