Isolation and structural characterization of monomeric and dimeric forms of replicative intermediates of Kilham rat virus DNA.
Two virus-specific species of newly synthesized DNA were isolated from rat fibroblast cell cultures infected with the Kilham rat virus (RV). These two DNA species were purified; their behavior on hydroxyapatite chromatography and their sedimentation coefficients in sucrose gradients were determined. One of the two species corresponds to the linear double-stranded form of the RV DNA, and the other corresponds to the dimeric duplex form. After denaturation, a fraction of both species showed an intramolecular renaturation; these molecules are composed of viral strand covalently linked to complementary strand. Models for the structure of both species are posposed. Both species may be considered as double-strand replicative intermediates of the single-stranded RV DNA.
D002460:Cell Line; D002499:Centrifugation, Density Gradient; D002845:Chromatography; D004261:DNA Replication; D004279:DNA, Viral / Q000032:analysis*; D006882:Hydroxyapatites; D008961:Models, Structural; D010321:Parvoviridae / Q000032:analysis*; D017992:Parvovirus / Q000032:analysis*
D004279:DNA, Viral; D006882:Hydroxyapatites