In vitro translation of Harvey murine sarcoma virus RNA.
The viral RNA of the Harvey strain of murine sarcoma virus (Ha-SV), which does not encode for any known viral structural polypeptides, has been translated in a nuclease-digested, cell-free system. The major protein product of the in vitro translation reaction has a molecular weight of 21,000 and is initiated faithfully with [35S]formylmethionine from formyl-[35S]methionyl-tRNAFMET. This polypeptide is clearly distinct from the RNA of the Moloney strain of type C helper virus used to pseudotype the Ha-SV. The intensity of the 21,000-dalton polypeptide on gels correlates well to the concentration of Ha-SV RNA in different viral RNA preparations. These experiments indicate that a polypeptide marker for Ha-SV is now available for the first time. The possibility that this protein is the product of the rat portion of the Ha-SV genome is discussed.
D002460:Cell Line; D002474:Cell-Free System; D017988:Gammaretrovirus / Q000378:metabolism*; D006378:Helper Viruses / Q000378:metabolism; D008970:Molecular Weight; D008979:Moloney murine leukemia virus / Q000378:metabolism; D009239:N-Formylmethionine / Q000378:metabolism; D010452:Peptide Biosynthesis; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000378:metabolism*; D009053:Sarcoma Viruses, Murine / Q000378:metabolism*; D014764:Viral Proteins / Q000096:biosynthesis*
D012367:RNA, Viral; D014764:Viral Proteins; D009239:N-Formylmethionine