Buffer-exchange column for rapid separation of carcinoembryonic antigen from perchloric acid.
A Sephadex G-50 medium (Pharmacia) buffer-exchange column has been developed for rapidly changing the medium for carcinoembryonic antigen from perchloric acid to acetate buffer. Analytical recovery of the extracted antigen exceeded 95%. Plasma for analysis can be so prepared and samples ready for analysis within an hour. The technique also results in uniform assay volume, thus eliminating this variable from the assay.
D002021:Buffers; D002272:Carcinoembryonic Antigen / Q000302:isolation & purification*; D002850:Chromatography, Gel / Q000379:methods; D003956:Dialysis; D004492:Edetic Acid; D006801:Humans; D010472:Perchlorates
D002021:Buffers; D002272:Carcinoembryonic Antigen; D010472:Perchlorates; D004492:Edetic Acid