Effects of 5-bromodeoxyuridine on the ACTH-dependent mitochondrial biogenesis in cortical cells of fetal rat adrenals in tissue culture.
Cortical cells of fetal rat adrenals in tissue culture were treated with 5-bromodeoxyuridine (BrdU) during their proliferative phase and during ACTH stimulation when nuclear DNA synthesis has almost ceased. Pretreatment with 0.5 mug/ml/day of BrdU inhibited the ACTH-induced differentiation of cortical cells as well as the secretion of corticosterone and 18-OH-deoxycorticosterone (18-OHDOC). When nuclear DNA synthesis was suppressed and mitochondrial DNA synthesis was stimulated by ACTH BrdU addition (30 mug/ml/day) permitted normal untrastructural differentiation of cortical cells, except that the development of mitochondrial inner membranes was inhibited. Simultaneously mitochondrial inner membranes was inhibited. Simultaneously mitochondrial 11beta- and 18-hydroxylations were strongly inhibited while cytoplasmic 21-hydroxylation was not affected.
D000302:Adrenal Cortex / Q000196:embryology / Q000378:metabolism / Q000648:ultrastructure*; D000311:Adrenal Glands / Q000648:ultrastructure*; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology*; D000818:Animals; D001973:Bromodeoxyuridine / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D003345:Corticosterone / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis; D003900:Desoxycorticosterone / Q000378:metabolism; D008928:Mitochondria / Q000648:ultrastructure*; D009024:Morphogenesis / Q000187:drug effects; D011374:Progesterone / Q000378:metabolism; D051381:Rats
D003900:Desoxycorticosterone; D011374:Progesterone; D000324:Adrenocorticotropic Hormone; D004247:DNA; D001973:Bromodeoxyuridine; D003345:Corticosterone