[Molecular mechanism of the action of lucanthone on tumor and normal cells].
The standard analytical and preparative electrophoresis in polyacrylamide gel did not reveal qualitative differences in the fraction composition of c-RNA of the ascitic tumour cells (Zaidel hepatoma, Ehrlich carcinoma, NK/ly lymphoma) and normal liver cells. In vitro the tumour and normal cells efficiently incorporated the labeled uridine into the all main classes of c-RNA (4S, 18S, 28S, fractions above 28S), the process being more intensive in the tumour cells. The presence of tumours in the animal organism had a significant effect on RNA biosynthesis in the liver, the effect being dependent on the tumor nature. Lucantone (miracyl D) had practically no effect in vitro on the quantitative content of the summation c-RNA and its fractions in all cells studied. However, it markedly inhibited the metabolism of the main fractions of c-RNA in the cells of Zaidel hepatoma and Ehrlich carcinoma. The effect of lucatone in the cells of NK/ly lymphoma was contrary.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000188:drug therapy / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy / Q000378:metabolism; D003593:Cytoplasm / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000187:drug effects*; D008113:Liver Neoplasms / Q000188:drug therapy / Q000378:metabolism; D008154:Lucanthone / Q000494:pharmacology*; D008223:Lymphoma / Q000188:drug therapy / Q000378:metabolism; D008297:Male; D051379:Mice; D008967:Molecular Biology; D009374:Neoplasms, Experimental / Q000188:drug therapy* / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D012334:RNA, Neoplasm / Q000378:metabolism; D051381:Rats; D014316:Tritium
D012334:RNA, Neoplasm; D014316:Tritium; D012313:RNA; D008154:Lucanthone
Molekuliarnyĭ mekhanizm deĭstviia liukantona na opukholevye i normal'nye kletki