title: Extraction of skin test activity from Coccidioides immitis mycelia by water, perchloric acid, and aqueous phenol extraction.
abstract: Water, perchloric acid extracts, and fractions of partially defatted whole mycelia of Coccidioides immitis contained delayed skin test activity when tested in C. immitis-infected guinea pits. Aqueous phenol extraction of these fractions resulted in partitioning of activity between aqueous-soluble and phenol-soluble fractions; activity was found to be water soluble after removal of phenol by extensive dialysis. Highest specific activity skin test antigen was invariably found in the phenol-soluble phase, water-soluble fraction. Material of equivalent activity could also be extracted directly from the defatted mycelia. Skin test active fractions contained glucose, mannose, 3-O-methylmannose, glucosamine, and amino acids.
mesh_terms: D000818:Animals; D000946:Antigens, Fungal / Q000032:analysis / Q000302:isolation & purification*; D003045:Coccidioides / Q000276:immunology*; D005944:Glucosamine / Q000032:analysis; D005947:Glucose / Q000032:analysis; D006168:Guinea Pigs; D008358:Mannose / Q000032:analysis; D010472:Perchlorates; D010636:Phenols; D012882:Skin Tests; D012995:Solubility; D014867:Water
chemical_list: D000946:Antigens, Fungal; D010472:Perchlorates; D010636:Phenols; D014867:Water; D005947:Glucose; D005944:Glucosamine; D008358:Mannose