title: Collagenase immunolocalization in cultures of rheumatoid synovial cells.
abstract: Cultures of rheumatoid synovial cells that have been enzymatically dissociated and are adherent to a culture vessel are morphologically heterogeneous. When these cells are cultured on a collagenous substrate for 2 to 6 days at 37 degrees C in serum-free medium, they produce collagenase. A monospecific antibody to human collagenase has localized the enzyme extracellularly around cytoplasmic extensions of dendritic cells and intracellularly within a few macrophage-like and fibroblast-like cells.
mesh_terms: D001172:Arthritis, Rheumatoid / Q000201:enzymology*; D002478:Cells, Cultured; D005347:Fibroblasts / Q000201:enzymology; D006801:Humans; D003012:Microbial Collagenase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013582:Synovial Fluid / Q000166:cytology
chemical_list: D003012:Microbial Collagenase