[Iso-octane soluble complex of cytochrome c with bovine brain phospholipids].
The conditions are determined for formation of the isooctane-soluble complex of cytochrome c with bull brain phospholipids. Molecular ratio protein/lipid in lipocytochrome c is 1 : 20 divided by 30. Ferri- and ferroforms of protein have different affinity to phospholipids. The ferrocytochrome c in the complex is subjected to self-oxidation in the absence of redox agents. Interaction of protein with phospholipids in water phase is shown to be of electrostatic nature. The cytochrome c conformation in water solution and in the complex does not differ significantly. But the mode of packing of lipid and protein molecules in the isooctane-soluble complex depends upon the degree of oxidation as has been shown by sedimentation methods. Cytochrome c maintains his conformation in a complex with phospholipids and retains the ability to redox reactions.
D000473:Alkanes; D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D003574:Cytochrome c Group; D010084:Oxidation-Reduction; D010743:Phospholipids / Q000302:isolation & purification; D012995:Solubility
D000473:Alkanes; D003574:Cytochrome c Group; D010743:Phospholipids
Doslidzhennia rozchinnoho v izooktani kompleksu tsytokhromu c z fosfolipidamy mozku byka