[Obtaining pig pulmonary macrophages for the cultivation of swine cytomegaloviruses].
A method of obtaining and cultivating pig pulmonary macrophages (PPM) is described and documented. The results obtained with its use in the cultivation of pig cytomegalovirus of the ADRI-1 reference strain are shown. In PPM cell cultures, the virus was demonstrated cytologically cytologically and by electron microscopy. After 18 passages on cell cultures, the virus, applied intranasally, infectious to colostrum.
D000818:Animals; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000254:growth & development*; D008168:Lung; D008264:Macrophages / Q000382:microbiology*; D013552:Swine / Q000382:microbiology*
Získávání prasecích plicních makrofágů pro kultivaci prasecího cytomegaloviru