Identification of the minor guanylated tRNA of rabbit reticulocytes.
Two of the tRNA's found in rabbit reticulocytes are substrates for a post-transcriptional modification leading to the incorporation of guanine into the polynucleotide chain. The major guanylated tRNA was previously identified as tRNA (His). In the present report we show that the minor guanylated tRNA is tRNA (Asn), and that just as in the case of tRNA (His), the guanine is located in an internal position. There are only two tRNA (Asn) in reticulocytes. We further show that one of these, the one that is not labeled with guanine, contains the hypermodified base known as Q. tRNA (Asn) does not contain Q.
D000818:Animals; D001216:Asparagine; D001483:Base Sequence; D006147:Guanine / Q000378:metabolism; D006150:Guanine Nucleotides / Q000032:analysis*; D010084:Oxidation-Reduction; D010504:Periodic Acid; D012343:RNA, Transfer / Q000097:blood* / Q000378:metabolism; D011817:Rabbits; D012156:Reticulocytes / Q000032:analysis*
D006150:Guanine Nucleotides; D010504:Periodic Acid; D006147:Guanine; D001216:Asparagine; D012343:RNA, Transfer