[Structurally functional organization of corticotropin: lipolytic and steroidogenic activity of some of its fragments].
The influence of ACTH fragments, possessing structural elements, common for certain groups of peptide hormones and kinins--"common" fragments and cluster of basic amino-acids--(Lys 17,18-ACTH 11-18-NH2--I; ACTH 11-13-NH2--II; NH2CO-ACTH18-20-NH2--III) on lipolytic effect of ACTH in rat isolated fat cells and on the steroidogenic effect of ACTH in isolated rat adrenal cells was studied. Fragment I exerts a steroidogenic effect (alpha=0,84) at concentrations of 1--100 microng/ml. At low concentrations (10(-8)--10(-3) microng/ml) fragment I potentiates ACTH-induced steroidogenesis. Fragment I has no effect on the lipolysis;however, it potentiates ACTH-induced lipolysis at concentrations of 10--100 microng/ml. The results obtained support our previous supposition that "common" fragments are essential secondary non-specific active sites of hormones.
D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000311:Adrenal Glands / Q000187:drug effects / Q000378:metabolism*; D000324:Adrenocorticotropic Hormone / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D050356:Lipid Metabolism; D010446:Peptide Fragments / Q000494:pharmacology; D051381:Rats; D013256:Steroids / Q000096:biosynthesis*; D013329:Structure-Activity Relationship
D010446:Peptide Fragments; D013256:Steroids; D000324:Adrenocorticotropic Hormone
Strukturno-funktsional'naia organizatsiia aktg: lipoliticheskaia i steroidogennaia aktivnost' nekotorykh fragmentov molekuly