The dissociation of transplantable tumors.
Four animal transplantable solid tumors, composed of varying morphologic architecture and intercellular specializations, were studied by light and electron microscopy. These tumors were dissociated into viable single cell populations using a combination of mechanical and enzymatic methods. The conditions necessary for optimal dissociation consisted of (a) preparation of the tumor to maximize the tissue surface area, (b) enzymatic digestion with continuous agitation and (c) additional agitation to release loosely attached cells. Other factors that influenced the dissociation were optimized and discussed.
D000231:Adenocarcinoma, Papillary / Q000473:pathology; D000818:Animals; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D005260:Female; D007365:Intercellular Junctions / Q000648:ultrastructure; D008297:Male; D008321:Mammary Glands, Animal; D008545:Melanoma / Q000473:pathology; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D003012:Microbial Collagenase; D009368:Neoplasm Transplantation; D009369:Neoplasms / Q000473:pathology; D014357:Trypsin
D014357:Trypsin; D003012:Microbial Collagenase