title: [Attempts to adapt BNK cells to cultivation in suspension and study of the antigenic properties of foot-and-mouth diseases viruses obtained from them].
abstract: Experiments were carried out to cultivate the foot-and-mouth disease virus under laboratory conditions in a suspension of the BHK-21 cell line. Results showed that the number of cells cultivated in a susfor 48 to 72 hours of incubation reaches up to 2.2 X 10(6). It was also found that certain conditions should be observed for the successful cultivation of the cells in suspension, such as aeration, pH, and respective temperature. It was demonstrated that foot-and-mouth diseases viruses obtained from BHK cells propagating in suspension possess good antigenic and immunogenic properties.
mesh_terms: D000818:Animals; D000956:Antigens, Viral; D005537:Aphthovirus / Q000276:immunology / Q000302:isolation & purification*; D002460:Cell Line; D005536:Foot-and-Mouth Disease / Q000517:prevention & control; D006168:Guinea Pigs; D008722:Methods; D014611:Vaccination; D014765:Viral Vaccines; D014776:Virus Cultivation
chemical_list: D000956:Antigens, Viral; D014765:Viral Vaccines
vernacular_title: Opiti za adaptirane na BNK kletki kum suspenzionno kultivirane i prouchvane antigennite kachestva na dobitite ot tiakh shapni virusi