[The use of certain new O-benzoquinone derivatives as acceptor substrates in enzymatic oxidation of NADH].
A possibility of the use of new aliphatic and aminoarylic o-benzoquinone derivatives as acceptor substrates in NADH oxidation catalyzed by NAD(P)H: (acceptor)-oxidoreductase (EC 1.6.99.2) was demonstrated. The kinetic mechanism of this reaction was studied in a monosubstrate approach and the effective kinetic constants were determined. The stability of these o-benzoquinone derivatives in the oxidation-reduction reactions, including anzymic reduction and subsequent auto-oxidation of their diphenolic forms by oxygen, was substantiated using spectrophotometric techniques.
D007700:Kinetics; D009243:NAD / Q000378:metabolism*; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D010084:Oxidation-Reduction; D011808:Quinone Reductases / Q000378:metabolism*; D011809:Quinones / Q000378:metabolism*; D013329:Structure-Activity Relationship
D011809:Quinones; D009243:NAD; D009247:NADH, NADPH Oxidoreductases; D011808:Quinone Reductases
Ispol'zovanie Novykh Proizvodnykh O-benzokhinona v kachestve aktseptiruiushchikh substratov pri fermentativnom okislenii NADH