Effects of cytochalasin B on low-density lipoproteins metabolism by cultured human fibroblasts.
The role of cytoplasmic microfilaments in the metabolism of low-density lipoprotein by human fibroblasts was studied with the aid of cytochalasin B. At concentrations of 5--40 nmol/ml cytochalasin increased the surface binding but decreased the endocytosis of 125I-labelled low-density lipoprotein. Subsequent studies indicated that these changes reflected a reduction of the rate of internalisation of low-density lipoprotein receptors. Independent inhibitory effects were also observed on low-density lipoprotein degradation and on the cellular release of the trichloroacetic acid-soluble degradation products.
D003571:Cytochalasin B / Q000494:pharmacology*; D003593:Cytoplasm / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004705:Endocytosis / Q000187:drug effects; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D008077:Lipoproteins, LDL / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects; D011955:Receptors, Drug / Q000378:metabolism
D008077:Lipoproteins, LDL; D011955:Receptors, Drug; D003571:Cytochalasin B