[The fructose induces "glycogenosis". III. Histochemical and morphometrical studies of glycogen metabolism in mouse liver after fructose overload (author's transl)].
BACKGROUND
After feeding fructose for 7 days rat liver cells show an accumulation of glycogen, a high activity of glucose-6-phosphatase combined with a SER- and RER-reduction. This result was reviewed by mouse liver cells using histochemical and morphometrical methods.


METHODS
60% fructose in drinking water was given mice as only nutritional source. Controls had free access to Altromin-R-standard diet and drinking water. Glycogen and glycogen metabolizing enzymes are demonstrated in the course of an 1-14 days fructose diet. After a 7 days diet liver tissue was analysed morphometrically.


CONCLUSIONS
Feeding of fructose leads to a high glycogen content, combined with a high activity of glycogen-phosphorylase and glucose-6-phosphatase in the liver parenchyma of mouse. Glycogen-synthetase activity falls to a low level. The SER and RER and the peroxisomes are reduced. The single volume of the hepatic nucleus is decreased and the hepatocellular chondrioma is transformed in a smaller number of larger mitochondria. Compared with the rate the analysed organelles and enzymes of mouse liver show only slight quantitative differences. The increase of glucose-6-phosphatase and simultaneous reduction of endoplasmic reticulum-membranes is illustrated by the dynamic structure of endoplasmic reticulum-membranes, which adapt to metabolic changes. The variable turnover of different parts of endoplasmic reticulum-membranes seems to be very important.
D000818:Animals; D004721:Endoplasmic Reticulum; D005632:Fructose; D005952:Glucose-6-Phosphatase / Q000032:analysis; D006008:Glycogen Storage Disease / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology; D008099:Liver / Q000201:enzymology / Q000378:metabolism / Q000473:pathology*; D008112:Liver Glycogen / Q000378:metabolism; D008297:Male; D051379:Mice
D008112:Liver Glycogen; D005632:Fructose; D005952:Glucose-6-Phosphatase
Die Fruktose-induzierte "Glykogenose". III. Histochemische und morphometrische Untersuchungen des Glykogenstoffwechsels in der Mäuseleber nach Fruktosebelastung.