title: An unusual defective genotype derived from herpes simplex virus strain ANG.
abstract: A defective genotype of herpes simplex virus strain Angelotti (HSV ANG) accumulates in the course of controlled serial high MOI virus passages representing 50-60% of the total number of mature virions in the seventh of these passages. Defective HSV ANG significantly differs from other defective HSV genotypes described so far: the DNA of the defective particles has the same buoyant density as nondefective DNA. In contrast to non-defective HSV ANG DNA, it is not attacked by the restriction endonucleases Eco R I, Hpa I and Hind III. Defective virions strongly suppress the formation of progeny virus. They do not interfere, however, with the synthesis of HSV-specified thymidine (TdR) kinase.
mesh_terms: D002460:Cell Line; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000032:analysis*; D005838:Genotype; D018139:Simplexvirus / Q000201:enzymology / Q000254:growth & development / Q000502:physiology*; D013937:Thymidine Kinase / Q000096:biosynthesis
chemical_list: D004279:DNA, Viral; D013937:Thymidine Kinase; D004262:DNA Restriction Enzymes