A functioning complex between tryptic fragments of cytochrome c. A route to the production of semisynthetic analogues.
We report the discovery of a functioning non-covalent complex between two peptides obtained from a limited tryptic digest of horse heart cytochrome c. We have used the phenomenon to produce three modified versions of the complex. We have replaced lysine-39 semisynthetically with ornithine in the first analogue, with p-fluorophenyl-alanine in the second, and removed it  entirely in the third.
D000818:Animals; D003574:Cytochrome c Group / Q000031:analogs & derivatives / Q000138:chemical synthesis; D006736:Horses; D008239:Lysine; D009206:Myocardium / Q000201:enzymology; D009952:Ornithine; D014357:Trypsin; D010135:p-Fluorophenylalanine
D003574:Cytochrome c Group; D010135:p-Fluorophenylalanine; D009952:Ornithine; D014357:Trypsin; D008239:Lysine