In vitro susceptibility of mink lung cells to the mouse mammary tumor virus.
Lung cells from mink embryos were infected in vitro with a purified mammary tumor virus isolated from RIII mouse milk. Specific virus antigen at the cell surface was detected by membrane immunofluorescence; B-type virions budding from the cell membrane were seen by electron microscopy. Nucleic acid hybridization confirmed replication and specificity of the virus produced.
D000818:Animals; D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D002462:Cell Membrane / Q000276:immunology / Q000648:ultrastructure; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D005260:Female; D008168:Lung / Q000382:microbiology; D008324:Mammary Tumor Virus, Mouse / Q000254:growth & development; D051379:Mice; D008907:Mink; D011247:Pregnancy; D014779:Virus Replication
D000951:Antigens, Neoplasm; D000956:Antigens, Viral