Cytochrome c-551.5 (c7) from Desulfuromonas acetoxidans.
Cytochrome c-551.5 of the anaerobic sulfur-reducing bacterium Desulfuromonas acetoxidans has been purified to homogeneity and characterized. It elicits absorption bands at 551.5, 522.5 and 418 nm in the reduced form; the absorptivity ratio Aalpha(red)/A280nm(ox) equals 3.8 for the pure preparation. The molecular weight was estimated to be 9800 by gel filtration. Determination of the amion acid composition and analysis of the N-terminal amino acid sequence showed the cytochrome to be identical with the threehaem cytochrome c-551.5 (c7) isolated from the syntrophic mixed culture Chloropseudomonas ethylica strain 2K. The occurrence of multihaem cytochromes c in bacteria is discussed.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D001051:Apoenzymes / Q000302:isolation & purification; D003574:Cytochrome c Group / Q000302:isolation & purification; D003901:Desulfovibrio / Q000201:enzymology*; D008970:Molecular Weight; D013053:Spectrophotometry; D013056:Spectrophotometry, Ultraviolet
D000596:Amino Acids; D001051:Apoenzymes; D003574:Cytochrome c Group