Genome modification in two lines of baby hamster kidney fibroblasts (BHK-21).
Reasons for the different levels of 5-methyl cytosine encountered in the DNA of two baby hamsters kidney fibroblast lines, BHK-21/C13 and BHK-21/PyY have been investigated. From enzymic studies it does not seem that there are large numbers of potentially methylatable cytosine residues in the C13 line DNA which contains a lower level of 5-methyl cytosine. Rather it is possible that the difference may be due to the reiteration in the PyY strain of certain sequences containing 5-methyl cytosine which simply occur less frequently in the other line.
D002460:Cell Line; D002467:Cell Nucleus / Q000201:enzymology; D002472:Cell Transformation, Viral; D003596:Cytosine / Q000031:analogs & derivatives* / Q000378:metabolism; D004247:DNA / Q000378:metabolism*; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000378:metabolism; D007700:Kinetics; D009695:Nucleic Acid Renaturation; D011120:Polyomavirus
D003596:Cytosine; D004247:DNA; D004248:DNA (Cytosine-5-)-Methyltransferases