Methyldopa binding to cells in culture.
Cells from a rat hepatoma grown in culture were found to activate methyldopa to intermediates which are bound irreversibly to cellular proteins. The binding reaction was not inhibited by superoxide dismutase or allopurinol, but was strongly inhibited by ascorbic acid and glutathione. Methyldopa, paracetamol and furosemide were not mutagenic in the Salmonella/mammalian-microsome mutagenicity test.
D000082:Acetaminophen / Q000378:metabolism; D000493:Allopurinol / Q000494:pharmacology; D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D006528:Carcinoma, Hepatocellular / Q000378:metabolism; D002478:Cells, Cultured; D003676:Deferoxamine / Q000494:pharmacology; D004128:Dimethylnitrosamine / Q000378:metabolism; D004492:Edetic Acid / Q000494:pharmacology; D005665:Furosemide / Q000378:metabolism; D005978:Glutathione / Q000494:pharmacology; D008113:Liver Neoplasms / Q000378:metabolism; D008298:Maleates / Q000494:pharmacology; D008750:Methyldopa / Q000378:metabolism*; D009374:Neoplasms, Experimental / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects; D051381:Rats; D013482:Superoxide Dismutase / Q000494:pharmacology; D013997:Time Factors; D014970:Xanthines / Q000494:pharmacology
D008298:Maleates; D014970:Xanthines; D000082:Acetaminophen; D008750:Methyldopa; D000493:Allopurinol; D005665:Furosemide; D004492:Edetic Acid; D013482:Superoxide Dismutase; D005978:Glutathione; D003676:Deferoxamine; D004128:Dimethylnitrosamine; D001205:Ascorbic Acid