Rescue of Rous sarcoma virus (RSV) from RSV-transformed human embryonic cells by cell fusion with chick embryo fibroblasts using lysolecithin.
Heterokaryon formation and Rous sarcoma virus (RSV)-induction were studied by fusion of RSV-transformed human embryonic cells with chick embryo fibroblasts in the presence of lysolecithin. Heterokaryon formation was observed by autoradiography. RSV-induction was identified by focus formation, electron microscopy and density gradient centrifugation of 3H-uridine-labeled particles. The most effective concentration of lysolecithin for virus induction was 10 mug/10(6) cells/0.1 ml. Efficiency of lysolecithin in virus induction was not less than that of ultraviolet-inactivated Sendai virus (UV-HVJ).
D000818:Animals; D001345:Autoradiography; D001358:Avian Sarcoma Viruses; D002459:Cell Fusion; D002642:Chick Embryo; D005347:Fibroblasts / Q000648:ultrastructure; D066298:In Vitro Techniques; D008244:Lysophosphatidylcholines; D014776:Virus Cultivation
D008244:Lysophosphatidylcholines