Lasar flow cytophotometric immunoperoxidase detection of herpes simplex virus type 2 antigens in infected cultured human cells.
Human cells in culture (HEp-2) were infected with herpes simplex virus type 2 (HSV-2) at multiplicities of infection varying from 0.2 to 10, and fixed 6, 12, 18 and 24 hr after infection. Infection-related antigens were detected by an indirect double antibody (peroxidase conjugated goat anti-rabbit to rabbit anti-herpes simplex virus type 2) immunoenzymatic staining reaction that rendered infection-related antigens visible by light microscopy. A corresponding series of laser flow cytophotometric experiments yielded reproducible large-angle (1-19 degrees) laser-light scattering distributions that depended upon multiplicities of infection and the location of the infection-related antigens in the infected cells.
D000956:Antigens, Viral / Q000032:analysis*; D002460:Cell Line; D002467:Cell Nucleus / Q000276:immunology*; D003593:Cytoplasm / Q000276:immunology*; D007124:Immunoenzyme Techniques; D007834:Lasers; D018139:Simplexvirus / Q000276:immunology*; D013053:Spectrophotometry; D013997:Time Factors
D000956:Antigens, Viral