A method using toluene-2,4-diisocyanate and glutaraldehyde to stabilize and conjugate antigens to erythrocytes for use in passive hemagglutination tests.
Erythrocytes were stabilized and activated for conjugation by simultaneous treatment with toluene-2,4-diisocyanate and glutaraldehyde. Following conjugation with an antigen, these cells were suitable for use in passive hemagglutination tests and could be stored in either hypo- or hyper-tonic solutions at 4 degrees C for at least 3 months without hemolysis or loss of agglutinating properties. Furthermore, these cells can be lyophilized and reconstituted with minimal loss of conjugation ability or of agglutination titer.
D000447:Aldehydes; D000818:Animals; D000941:Antigens; D001665:Binding Sites; D001793:Blood Preservation; D003485:Cyanates; D004912:Erythrocytes / Q000276:immunology*; D005976:Glutaral; D006386:Hemagglutination Tests / Q000379:methods; D006461:Hemolysis; D006801:Humans; D008297:Male; D011817:Rabbits; D014051:Toluene 2,4-Diisocyanate
D000447:Aldehydes; D000941:Antigens; D003485:Cyanates; D014051:Toluene 2,4-Diisocyanate; D005976:Glutaral