Neoplastic transformation of hamster brain cells in vitro by polyoma virus.
The transformation of cultivated hamster brain cells by polyoma virus is reported. The transformed cell line contained polyoma virus-specific nuclear, surface and transplantation antigens. Subcutaneous and intracranial inoculations revealed high tumorigenicity of the cells. Brain-specific S 100 protein was found in these tumors with immuno-peroxidase staining, suggesting that they were of a nervous nature. Both in vivo and in vitro, the cells had glial features as studied by phase contrast, light and electron microscopy. Type-H virus-like particles were found in the tumor cells and might have played a role in the viral transformation.
D000818:Animals; D000956:Antigens, Viral; D001921:Brain; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D011120:Polyomavirus / Q000276:immunology; D009418:S100 Proteins / Q000378:metabolism; D014412:Tumor Virus Infections / Q000276:immunology
D000956:Antigens, Viral; D009418:S100 Proteins