Pilot scale purification of alpha-galactosidase A from Cohn fraction IV-1 of human plasma.
Human plasma alpha-galactosidase A (alpha-D-galactoside galactohydrolase, EC 3.2.1.22) was purified 7000-fold over plasma levels from Cohn Fraction IV-1. The yield per kg starting material averaged 11 000 units (nmol galactose liberated per h) and the specific activity was about 600 units per mg protein with 4-methylumbelliferyl-alpha-D-galactoside. The ratio of 4-methylumbelliferyl-alpha-galactosidase to ceramide trihexosidase activities was 6.2. Both activities were heat labile and exhibited the same relative mobilities on polyacrylamide gel electrophoresis. Enzymatic activity was stable for at least 4 months at 4 and -20 degrees C. The endotoxin concentration of this preparation averaged 0.26 mg per mg protein.
D005696:Galactosidases / Q000097:blood*; D006801:Humans; D007700:Kinetics; D013696:Temperature; D000519:alpha-Galactosidase / Q000097:blood* / Q000302:isolation & purification
D005696:Galactosidases; D000519:alpha-Galactosidase