title: Routine diagnosis of human rotaviruses in stools.
abstract: Electron microscopy, immune electron microscopy and complement fixation as methods of detecting rotavirus in the stools of young children with gastroenteritis were compared in a blind study during the winter of 1975-6. Complement fixation was the simplest to perform, was as sensitive as the other two, and allowed a quantitative measurement of viral excretion. Absorption of faecal extracts with fetal calf serum usually removed the anticomplementary activity of faecal extracts.
mesh_terms: D002648:Child; D003168:Complement Fixation Tests; D003967:Diarrhea / Q000209:etiology; D005243:Feces / Q000382:microbiology*; D006801:Humans; D012328:RNA Viruses / Q000302:isolation & purification*; D012401:Rotavirus / Q000302:isolation & purification* / Q000648:ultrastructure; D014777:Virus Diseases / Q000175:diagnosis*