Phenotypic modification of SV40-transformed hamster lymphoid cells in vivo.
Explants of simian virus 40 (SV40)-induced lymphoid tumors yield SV40-T-antigen-positive derivatives that differ from GD248 lymphocytes propagated in suspension culture, (or in vivo), in the following respects: polygonal shape, adhesion to culture substrates in vitro, phagocytic capacity, lack of immunoglobulin and a chromosome complement at least twice that of GD248 lymphocytes. When GD-248 lymphocytes are propagated as suspension in vitro, no such adherent variants can be detected. However, sequential in vivo passage of GD248 lymphocytes obtained from the suspension-culture lines also yield adherent cell lines upon explanation in vitro. Injection of adherent cells into hamsters produces tumors with histological features of reticulum cell sarcoma.
D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis; D002448:Cell Adhesion; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D006224:Cricetinae; D008214:Lymphocytes / Q000276:immunology / Q000648:ultrastructure*; D008647:Mesocricetus; D009374:Neoplasms, Experimental / Q000276:immunology / Q000648:ultrastructure*; D010641:Phenotype; D013539:Simian virus 40 / Q000254:growth & development / Q000276:immunology
D000951:Antigens, Neoplasm; D000956:Antigens, Viral