The location of the polio genome protein in viral RNAs and its implication for RNA synthesis.
Evidence is presented that a small protein (VPg) is covalently attached to the 5'-terminal oligonucleotide VPg-pU-U-A-A-A-A-C-A-Gp of the polio genome, to nascent strands of the polio replicative intermediate and to poly(U) of minus strands. A model of polio RNA replication is proposed implicating VPg in initiation of RNA symthesis, possibly as primer.
D009841:Oligonucleotides / Q000302:isolation & purification; D017955:Poliovirus / Q000378:metabolism*; D011485:Protein Binding; D012333:RNA, Messenger / Q000378:metabolism; D012367:RNA, Viral / Q000096:biosynthesis / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism*; D014779:Virus Replication
D009841:Oligonucleotides; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins