[Effect of carboxycathepsin on biological activity of bradykinin].
An effect of carboxycathepsin (peptidyl-dipeptidase EC 3.4.15.1) from bovine kidney on bradikinine was studied. The bradikinine activity was determined by monitoring the rat uterine horn retraction. Carboxycathepsin caused rapid inactivation of bradikinine (440-500 mcg of bradikinine/mg/min). The rate of bradikinine inactivation depended on the incubation time, content of the enzyme and substrate in the samples. In presence of EDTA carboxycathepsin did not inactivate bradikinine.
D000818:Animals; D001920:Bradykinin / Q000032:analysis / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D002417:Cattle; D003864:Depression, Chemical; D004492:Edetic Acid / Q000494:pharmacology; D005260:Female; D066298:In Vitro Techniques; D009130:Muscle, Smooth / Q000187:drug effects; D007703:Peptidyl-Dipeptidase A / Q000494:pharmacology*; D051381:Rats; D014599:Uterus / Q000187:drug effects
D004492:Edetic Acid; D007703:Peptidyl-Dipeptidase A; D001920:Bradykinin
Deĭstvie karboksitkatepsina na biologicheskuiu aktivnost' bradikinina