Studies on myocardial mitochondria in failing dog hearts: studies by electron spin resonance (ESR) spectrometry.
The formation of free radicals, which was represented by the concentration of free radicals in state 4 respiration, and the respiratory control ratio were studied in the myocardial mitochondria isolated from dog hearts that were failing due to aortic constriction. Both formation and respiratory control ratio were lowered, compared to each control value, in acute and chronic heart failure. A positive correlation was recognized between free radicals in state 4 respiration and respiratory control ratio. From these results it was concluded that there was uncoupling of oxidative phosphorylation in the mitochondria isolated from failing dog hearts. Measurement of free radicals in state 4 respiration, as well as respiratory control ratio, is a useful method for evaluation of the function of oxidative phosphorylation.
D000818:Animals; D002908:Chronic Disease; D004285:Dogs; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals; D006333:Heart Failure / Q000378:metabolism*; D008929:Mitochondria, Heart / Q000378:metabolism*; D010101:Oxygen Consumption
D005609:Free Radicals