title: Energy requirements for the action of staphylococcin 1580 in Staphyloccus aureus.
abstract: 1. Starved cells of a glucose-grown strain of Staphylococcus aureus are resistant to the action of staphylococcin 1580. Reinitiation of sensitivity is readily obtained upon the addition of glucose, but only weakly with L-lactate, although the latter induces higher ATP levels and supports L-glutamic acid uptake better than glucose does. The NADH/NAD+ ratio correlates with the staphylococcin sensitivity. 2. Starved pyruvate-grown cells remain partially susceptible and full sensitivity is restored both in the presence of glucose and L-lactate. 3. Arsenate but not dicyclohexylcarbodiimide (DCCD) blocks the reinitiation of sensitivity in the presence of glucose. Both arsenate and DCCD block sensitivity in the presence of L-lactate. 4. Aerobically grown cells are sensitive to staphylococcin 1580 under anaerobic conditions. Anaerobically grown cells are less susceptible, but sensitivity can be restored by glucose and also by L-lactate plus nitrate when cells are previously induced for nitrate reductase. 5. Starved cells of a mutant strain defective in the maintenance of a high-energy state of the membrane are normally sensitive in the presence of glucose, but resistant in the presence of L-lactate. A strain lacking a functional respiratory chain (men-) is also sensitive with glucose but resistant in the presence of L-lactate. 6. It is concluded that the initiation of the staphylococcin 1580 action is under control of a mechanism regulating the energy flow in the cell, and involving the presence of a high-energy phosphorylated compound.
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D001430:Bacteriocins / Q000494:pharmacology*; D004352:Drug Resistance, Microbial; D004734:Energy Metabolism / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology; D005971:Glutamates / Q000378:metabolism; D007773:Lactates / Q000494:pharmacology; D009243:NAD / Q000378:metabolism; D013210:Staphylococcus; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000378:metabolism; D013237:Stereoisomerism
chemical_list: D001430:Bacteriocins; D005971:Glutamates; D007773:Lactates; D009243:NAD; C017724:staphylococcin; D000255:Adenosine Triphosphate; D005947:Glucose