Cyclic AMP and the mechanism of leucocyte lysosomal enzyme release during an immediate hypersensitivity reaction in vivo.
The pleural cavity of rats was used to study the effect of altering leucocyte cyclic AMP content on the release of B-glucuronidase activity during an immediate hypersensitivity reaction. The effect on intravenous colchicine was also studied. Despite an increase of 135 to 235% in leucocyte cyclic AMP content no decrease in B-glucuronidase release was observed. Similarly, colchicine had no effect on enzyme release. It was concluded that the cyclic nucleotides and leucocyte microtubules may have no significant role to play in the release of lysosomal enzymes during acute inflammation in vivo.
D000818:Animals; D001183:Arthus Reaction / Q000097:blood* / Q000201:enzymology; D003994:Bucladesine / Q000494:pharmacology; D003078:Colchicine / Q000494:pharmacology; D000242:Cyclic AMP / Q000097:blood*; D006152:Cyclic GMP / Q000097:blood; D005966:Glucuronidase / Q000097:blood; D007770:L-Lactate Dehydrogenase / Q000097:blood; D007962:Leukocytes / Q000201:enzymology / Q000378:metabolism*; D008247:Lysosomes / Q000201:enzymology; D008297:Male; D051381:Rats; D013806:Theophylline / Q000494:pharmacology
D003994:Bucladesine; D013806:Theophylline; D000242:Cyclic AMP; D007770:L-Lactate Dehydrogenase; D005966:Glucuronidase; D006152:Cyclic GMP; D003078:Colchicine