Virus-specific markers and virus-like particles in cell lines of tumors produced by CELO virus in Syrian golden hamsters.
Cell lines were established from 2 primary hepatocellular carcinomas (HC's) and 3 sarcomas produced in Syrian golden hamsters inoculated as newborns with chicken embryo lethal orphan (CELO) virus. Cell lines from 2 sarcomas (COT, CMT) and 1 HC (CEHEP) produced CELO virus-specific T-antigen. The antigen was not detected in cells of the third sarcoma line (RCT) until they had undergone more than 34 passages in vitro. Although 5-10% of cells in the second HC line (CILT/2) contained T-antigen during early passages, it was not demonstrable after the fifth subculture. Nevertheless, cells of both HC lines possessed CELO virus tumor-specific transplantation antigen. All 5 cell lines also contained hamster type R particles, and both HC lines had type C and intracytoplasmic type A particles. The percentage of carcinoma cells producing type R particles increased during cultivation in vitro, whereas the number of cells with type A particles decreased. Treatment with dibutyryl cyclic AMP and theophylline enhanced the number of cells producing type C particles in 1 HC line and type R particles in 2 sarcoma lines.
D000256:Adenoviridae / Q000302:isolation & purification*; D000257:Adenoviridae Infections / Q000209:etiology*; D000293:Adolescent; D000818:Animals; D000956:Antigens, Viral; D000259:Aviadenovirus / Q000276:immunology / Q000302:isolation & purification*; D003994:Bucladesine / Q000494:pharmacology; D006528:Carcinoma, Hepatocellular / Q000209:etiology* / Q000648:ultrastructure; D002460:Cell Line; D006224:Cricetinae; D006649:Histocompatibility Antigens; D006801:Humans; D007181:Inclusion Bodies, Viral; D008113:Liver Neoplasms / Q000209:etiology* / Q000648:ultrastructure; D008647:Mesocricetus; D009374:Neoplasms, Experimental / Q000209:etiology; D012513:Sarcoma, Experimental / Q000209:etiology*; D014779:Virus Replication / Q000187:drug effects
D000956:Antigens, Viral; D006649:Histocompatibility Antigens; D003994:Bucladesine