[Histochemical and quantitative methods in the biopsy examination of skeletal muscles].
A complex bioptic examination of skeletal muscles as carried out in 280 excised samples has been subjected to a critical analysis. The examination suggested consists of histological and histochemical processing of native cryostat sections. Besides conventional staining methods the techniques recommended include the reaction for detection of myosin ATP-ase with and without acid preincubation, and reactions for NADH tertazolium reductase, acid phosphatase, nonspecific esterase, glycogen and lipids. Sections stained for ATP-ase are suitable for quantitative examinations such as assessing the diameters of muscle fibres.
D000251:Adenosine Triphosphatases / Q000378:metabolism; D001706:Biopsy / Q000379:methods*; D003576:Electron Transport Complex IV / Q000378:metabolism; D006651:Histocytochemistry / Q000379:methods; D006801:Humans; D009132:Muscles / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D009218:Myosins / Q000378:metabolism; D006005:Phosphorylases / Q000378:metabolism; D013385:Succinate Dehydrogenase / Q000378:metabolism
D013385:Succinate Dehydrogenase; D003576:Electron Transport Complex IV; D006005:Phosphorylases; D000251:Adenosine Triphosphatases; D009218:Myosins
Histochemické a kvantitativni metody v bioptickém vysetrování kosterního svalstva.