Isolation of T-lymphocytes from disaggregated tumors, with high purity and good percentage recovery.
A combination of two cell separation methods was utilized for the isolation of thymus-derived lymphocytes (TL) from enzymatically disaggregated tumors. Passage through Sephadex G-10-glass bead columns to remove adherent cell types followed by exposure to IgG-coated sheep red blood cell monolayers for removal of Fc receptor-bearing inflammatory cells provided functional TL suspensions of high purity with good percentage recovery.
D000818:Animals; D002460:Cell Line; D002469:Cell Separation / Q000379:methods*; D008297:Male; D008748:Methylcholanthrene; D051379:Mice; D008807:Mice, Inbred BALB C; D008979:Moloney murine leukemia virus; D009374:Neoplasms, Experimental / Q000209:etiology / Q000473:pathology*; D012513:Sarcoma, Experimental / Q000209:etiology; D013539:Simian virus 40; D013601:T-Lymphocytes / Q000473:pathology
D008748:Methylcholanthrene