Studies on the effect of quartz, bentonite and coal dust mixtures on macrophages in vitro.
The effect of quartz, bentonite and coal dusts as well as the effect of the artificial mixture of these dusts on TTC reduction and extra-and intra-cellular lactate dehydrogenase activity in peritoneal rat macrophages was determined in vitro. The cell-membrane-damaging effect of quartz caused a significant extracellular release of lactate dehydrogenase. Bentonite caused no extracellular enzyme release, which leads us to believe that the biological effect of this dust is shown by decrease in intra-cellular lactate dehydrogenase activity. TTC reduction was inhibited equally by quartz and qentonite. In mixtures of quartz (60%)-bentonite (40%) dust the specific effect of quartz was inhibited by bentonite in vitro and also in vivo. We obtained the same results with coal-quartz-bentonite dust mixtures in vitro. Our experiments show that comparison of the biological effects of artificial dust mixtures and airborne dust samples is justified, and prove that performing various examinations simultaneously give fuller particulars on the probable biological effect of mineral dusts.
D000818:Animals; D001546:Bentonite / Q000494:pharmacology*; D003031:Coal; D004391:Dust; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects* / Q000201:enzymology; D008297:Male; D011791:Quartz / Q000494:pharmacology*; D051381:Rats; D012822:Silicon Dioxide / Q000494:pharmacology*; D013730:Terphenyl Compounds
D003031:Coal; D004391:Dust; D013730:Terphenyl Compounds; D001546:Bentonite; D011791:Quartz; D012822:Silicon Dioxide; D007770:L-Lactate Dehydrogenase