Characterization of SA12 as a simian virus 40-related papovavirus of chacma baboons.
SA12 virus, originally isolated from an uninoculated South African vervet monkey kidney culture, was identified as a new member of the simian virus 40 (SV40)-polyoma subgroup of papovaviruses. The virus produced a cytopathic effect with nuclear enlargement in primary rhesus kidney cells. The virion had papovavirus morphology and a diameter of 44 to 45 nm. The DNA of the virus was a circular, double-stranded, superhelical molecule with a mean length 101% that of SV40 DNA and an estimated molecular weight of 3.3 X 10(6). The virus was found to be unrelated to other papovaviruses by neutralization, immune electron microscopy, and immunofluorescence tests with antiviral sera. SA12 virus-infected cells exhibited a capsid antigen, which has recently been found to be common to viruses of the SV40-polyoma subgroup. The virus readily transformed kideny cells from 10-day-old hamsters. Inoculation of transformed cells produced tumors in 3- to 4-week-old hamsters. The T antigens of SA12 and SV40 viruses were strongly and reciprocally cross-reactive. A high proportion of the sera of chacma baboons, Papio ursinus, and a comparatively lower proportion of the sera of vervet monkeys, Cercopithecus pygerythrus, had neutralizing antibodies to SA12 virus
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D000951:Antigens, Neoplasm / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D003429:Cross Reactions; D004270:DNA, Circular / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis; D000882:Haplorhini; D008970:Molecular Weight; D027383:Papillomaviridae / Q000276:immunology / Q000302:isolation & purification* / Q000648:ultrastructure; D010215:Papio / Q000276:immunology / Q000382:microbiology*; D027382:Polyomaviridae; D013539:Simian virus 40 / Q000276:immunology
D000914:Antibodies, Viral; D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D004270:DNA, Circular; D004279:DNA, Viral