title: Restriction and modification in Bacillus subtilis. Localization of the methylated nucleotide in the BsuRI recognition sequence.
abstract: Calf thymus DNA was methylated in vitro with cell extracts of Bacillus subtilis OG3R (r+m+) and S-adenosyl[Me-3H]methionine. After depurination of the [3H]methylated DNA, the analysis of the pyrimidine dinucleotides revealed the following positions of the methylated nucleosides (indicated by an asterisk) within the BsuRI recognition sequence: 5' dG--dG--dC--dC dC--dC--dG--dG 5'.
mesh_terms: D001412:Bacillus subtilis / Q000378:metabolism*; D001483:Base Sequence; D004247:DNA; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D008745:Methylation; D010727:Phosphoric Diester Hydrolases
chemical_list: D004247:DNA; D004262:DNA Restriction Enzymes; D010727:Phosphoric Diester Hydrolases