[Measurement of cyclic AMP in the islets of Langerhans of newborn rats. Effect of amino acids].
Radioimmunoassay of cyclic AMP (cAMP) in the islets of Langerhans from 48-64 h old Rats was performed after succinylation of the samples. cAMP was detected at 0.03 nM. The cAMP content of islets increases when L-arginine, L-lysine and L alanine are added together in the incubation medium at a concentration of 5-10 mM each. When phosphodiesterase is inhibited by theophylline the three amino acids considerably increase the cAMP content of islets. Thus an increase in cAMP content of the islets was observed with a concentration of amino acids which is efficient in stimulating the insulin and glucagon secretion.
D000409:Alanine / Q000494:pharmacology; D000596:Amino Acids / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn; D001120:Arginine / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism*; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D008239:Lysine / Q000494:pharmacology; D051381:Rats; D013386:Succinates; D013806:Theophylline / Q000494:pharmacology
D000596:Amino Acids; D013386:Succinates; D001120:Arginine; D013806:Theophylline; D000242:Cyclic AMP; D008239:Lysine; D000409:Alanine
Mesure de l'AMP cyclique dans les ilots de Langerhans du Rat nouveau-né. Effet des acides aminés.