[Complementary RNA in the chicken sarcoma cells and Rous sarcoma virus].
The subcellular localization in chicken Rous sarcoma of nucleotide sequence, complementary to Rous sarcoma virus RNA was examined by RNA/RNA molecular hybridization. The preparations of radioiodinated virion RNA were annealed with RNAs from different fractions (nuclei, mitochondria, free and membrane-bound polyribosomes) isolated from chicken Rous sarcoma. Formation of RNA-ase resistant hybrids between the viral 125I-RNA and RNA from the mitochondria and membrane-bound polyribosomes was revealed. The latter were characterized by a higher relative redundancy of nucleotide sequences complementary to virion RNA than that in the former, by factor 446. The role of complementary ribonucleotide sequences is discussed.
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics*; D002645:Chickens; D008928:Mitochondria / Q000032:analysis; D009693:Nucleic Acid Hybridization; D011132:Polyribosomes / Q000032:analysis; D012334:RNA, Neoplasm / Q000302:isolation & purification*; D012367:RNA, Viral / Q000302:isolation & purification*; D001357:Sarcoma, Avian / Q000235:genetics* / Q000382:microbiology / Q000473:pathology; D014771:Virion / Q000235:genetics
D012334:RNA, Neoplasm; D012367:RNA, Viral
Komplementarnost' ribonulleinovykh kislot v kletkakh sarkomy tsypliat i viruse sarkomy Rausa.