title: Effect of dilution on recovery of bacteria from blood.
abstract: The multiplication rate of bacteria in undiluted blood containing sodium polyanethol sulfonate was compared with growth rates obtained in dilutions of blood ranging from 1:2 to 1:8. Although all organisms tested grew in the undiluted blood, increased growth rates were seen in the 1:2 dilution. Further dilution resulted in growth rates equivalent to that obtained with the 1:2 dilution. In view of these results, we question the present recommendations that blood be diluted 1:10 or 1:20.
mesh_terms: D001419:Bacteria / Q000254:growth & development / Q000302:isolation & purification*; D001441:Bacteroides fragilis / Q000254:growth & development; D001769:Blood / Q000382:microbiology*; D002455:Cell Division; D003470:Culture Media; D004926:Escherichia coli / Q000254:growth & development; D006801:Humans; D011074:Polyanetholesulfonate; D011550:Pseudomonas aeruginosa / Q000254:growth & development; D013045:Species Specificity; D013211:Staphylococcus aureus / Q000254:growth & development; D013291:Streptococcus / Q000254:growth & development
chemical_list: D003470:Culture Media; D011074:Polyanetholesulfonate