Partial purification and characterization of the mRNA for rat preproinsulin.
Electrophoretically homogeneous messenger RNA for rat preproinsulin has been prepared from an islet cell tumor by the use of oligo(dT(-cellulose chromatography and sucrose density gradient centrifugation. The molecular weight of the mRNA is about 210,000, as determined by polyacrylamide gel electrophoresis in formamide, and its sedimentation coefficient is 9.3 S in sucrose gradients containing 0.2 M NaCl. These results indicate that the synthesis of preproinsulin is directed by a monocistronic mRNA 600 nucleotides in length and requires approximately 55% of the molecule's coding capacity.
D007516:Adenoma, Islet Cell / Q000032:analysis*; D000818:Animals; D002499:Centrifugation, Density Gradient; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight; D009374:Neoplasms, Experimental / Q000032:analysis; D011384:Proinsulin; D011498:Protein Precursors; D012333:RNA, Messenger / Q000302:isolation & purification; D051381:Rats
D011498:Protein Precursors; D012333:RNA, Messenger; D011384:Proinsulin