Preparation and properties of N6-monobutyryl adenosine 5'-monophosphate. A major hepatic metabolite of N6,O2'-dibutyryl cyclic adenosine 3':5'-monophosphate.
The synthesis and properties of N6-monobutyryl adenosine 5'-monophosphate are described. The properties of synthesized monobutyryl nucleotide have been compared to those of a metabolite recognized in previous studies (Castagna, M. C., Palmer, W.K., and Walsh, D.A. (1977) Arch. Biochem. Biophys. 181, 46-60) as the major radioactive product produced in the liver upon perfusion with N6,O2'-dibutyryl cyclic [3H]adenosine 3':5'-monophosphate. By the criteria of cochromatography on DEAE-cellulose and in three thin layer chromatographic systems and from equivalent rates of alkaline hydrolysis, N6-monobutyryl adenosine 5'-monophosphate has been identified as a major hepatic metabolite of N6,O2'-dibutyryl cyclic adenosine 3':5'-monophosphate.
D003994:Bucladesine / Q000378:metabolism*; D002852:Chromatography, Ion Exchange; D002855:Chromatography, Thin Layer; D000242:Cyclic AMP / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D004355:Drug Stability; D006868:Hydrolysis; D008099:Liver / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet
D003994:Bucladesine; D000242:Cyclic AMP