Size of the poly(A) sequences in encephalomyocarditis virus RNA.
Poly(A) sequences were demonstrated in the RNA of encephalomyocarditis (EMC) virus by affinity chromatography on poly(U)-Sepharose, treatment with RNases, and determination of the ability of the RNA to prime for reverse transcription. The size of the poly(A) sequences was estimated as 10-20 nucleotides in length by determination of the fraction resistant to pancreatic RNases plus T1 RNases, followed by analysis of electrophoretic mobility on polyacrylamide gels and of the AMP-to-adenosine ratio after alkaline hydrolysis. The poly(A) sequences are located at the 3' end of the RNA as shown by mild digestion by snake venom phosphodiesterase.
D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D004686:Encephalomyocarditis virus / Q000032:analysis*; D007739:L Cells; D011061:Poly A / Q000032:analysis*; D012367:RNA, Viral / Q000032:analysis* / Q000378:metabolism; D012260:Ribonucleases; D014158:Transcription, Genetic
D012367:RNA, Viral; D011061:Poly A; D012260:Ribonucleases