[Evaluation of the alginat membrane filter method for the Enterovirus concentration in water (author's transl)].
It is bound to use a concentration method for the research of enteroviruses in water. One of the concentration methods, the filtration on alginat membranes, avoids the problem of the virus elution from the filter, then the membrane is soluble in a sodium citrat buffer. This method was studied with different types of water (synthetic water, tap water and wastewater). -The recovery efficiency of Poliovirus I in clear waters (synthetic water and tap water) was poor, unsatisfying reproducible; the results are better for small virus concentrations. This efficiency may be slowly enhanced by the addition to the sample of aluminium chlorid at a final concentration of 5.10(-4) M. -The technical problems (brittleness of the membranes, slow filtrations speed, small sample volume), soon noticeable with clear waters, are more important in the case of trouble waters. Meanwhile the use of this filtration method gives better results for the recovery of Poliovirus I in wastewaters than the direct inoculation to cells cultures.
D000464:Alginates; D002021:Buffers; D002951:Citrates; D004770:Enterovirus / Q000302:isolation & purification*; D005374:Filtration / Q000295:instrumentation / Q000379:methods; D008567:Membranes, Artificial; D017955:Poliovirus / Q000302:isolation & purification; D014871:Water Microbiology; D014881:Water Supply / Q000032:analysis
D000464:Alginates; D002021:Buffers; D002951:Citrates; D008567:Membranes, Artificial
Alginat-Filter zur Konzentrierung von Enterovirus aus wässerigem Milieu - Grenzen der Methode.