The probable site of action of thenolytrifluoracetone on the respiratory chain.
1. It is shown that the electron-transfer inhibitor thenoyltrifluoroacetone abolishes a respiratory-chain electron-paramagnetic-resonance absorbance due to spin-spin interactions of ubisemiquinones at concentrations similar to those required for inhibition of succinate oxidation. 2. A specific site of interaction of thenoyltrifluoroacetone with the respiratory chain is proposed to be on the ubisemiquinone with which succinate dehydrogenase reacts. 3. Our results further demonstrate the close association of the HiPIP (high-potential iron-sulphur) centre of succinate dehydrogenase with ubisemiquinone.
D000818:Animals; D001665:Binding Sites; D004578:Electron Spin Resonance Spectroscopy; D004579:Electron Transport / Q000187:drug effects; D007506:Iron-Sulfur Proteins / Q000378:metabolism; D008931:Mitochondria, Muscle / Q000187:drug effects* / Q000378:metabolism; D013804:Thenoyltrifluoroacetone / Q000378:metabolism / Q000494:pharmacology*; D013876:Thiophenes / Q000494:pharmacology*; D014451:Ubiquinone / Q000378:metabolism*
D007506:Iron-Sulfur Proteins; D013876:Thiophenes; D014451:Ubiquinone; D013804:Thenoyltrifluoroacetone