Cytotoxic activity of methylene bis-nucleotides.
New facts relating to the mechanism of cytotoxic and antitumor activity of formaldehyde-treated RNA and its hydrolysates have been revealed. Synthetic methylene bis-nucleotides and, in particular, methylene bis-nucleosides have a cytotoxic effect on cultured human adenocarcinoma cells (line HCCL). The cytotoxic effect of methylene bis-derivatives of the guanine series was most pronounced. In addition, methylene bis-nucleotides were depbosphorylated in the cell culture medium. Chromosome aberrations were induced by alkaline hydrolysate of dialyzed formaldehyde-treated RNA in which 39% of purine nucleotdies were modified to methylene bis-derivatives.
D000249:Adenosine Monophosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D005557:Formaldehyde; D006157:Guanosine Monophosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D006801:Humans; D009374:Neoplasms, Experimental / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D009841:Oligonucleotides / Q000494:pharmacology*; D009843:Oligoribonucleotides / Q000378:metabolism / Q000494:pharmacology*; D012313:RNA
D009841:Oligonucleotides; D009843:Oligoribonucleotides; D005557:Formaldehyde; D000249:Adenosine Monophosphate; D012313:RNA; D006157:Guanosine Monophosphate