title: NAD nucleosidase of Agkistrodon bilineatus venom.
abstract: NAD nucleosidase (NAD glycohydrolase, EC 3.2.2.5) activity in Agkistrodon bilineatus venom was observed. Using the cyanide assay method at pH 7.9, lyophilized crude venom had an activity of 0.19 units/mg. Chromatography of the crude venom on DEAE Sephadex A-50 with ammonium acetate buffer by two stage elution yielded 13 fractions. Peak NAD nucleosidase activity occurred at Fraction X. Crude venom and Fraction X NAD nucleosidase activities were thermolabile.
mesh_terms: D000818:Animals; D007527:Isoenzymes / Q000032:analysis; D009244:NAD+ Nucleosidase / Q000032:analysis*; D014757:Viper Venoms / Q000032:analysis*
chemical_list: D007527:Isoenzymes; D014757:Viper Venoms; D009244:NAD+ Nucleosidase