Preparation and properties of nylon-tube-supported nicotinamide--adenine dinucleotide kinase.
Pigeon liver NAD kinase was covalently coupled to the inside surfaces of nylon tubes, both directly to the nylon and via polyamine specers Km values and the inactivation energy of the reaction changed upon immobilization, but the pH-dependence remained unaltered. The activities and stabilities of different preparations are compared.
D004800:Enzymes, Immobilized; D007700:Kinetics; D009243:NAD; D009757:Nylons; D010770:Phosphotransferases
D004800:Enzymes, Immobilized; D009757:Nylons; D009243:NAD; D010770:Phosphotransferases