title: Role of small molecules in regulation of D-serine deaminase synthesis.
abstract: Cyclic AMP is required for optimal synthesis of D-serine deaminase synthesis from dsdO+ templates and for optimal hyperinducible synthesis from low constitutive dsdO templates both in vitro and in vivo. Neither D-serine, cyclic AMP, nor dsdC activator has an effect on expression of a high constitutive dsdO template. The synthesis of the dsdC activator itself in vitro is independent of cyclic AMP. Guanosine tetraphosphate does not have a significant effect on in vitro D-serine deaminase synthesis from dsdO+ or dsdO templates. A previously described class of dsdO mutants showing partial catabolite sensitivity of constitutive D-serine deaminase synthesis proved to be low dsdO types. They all contain a low constitutive dsdC mutation; the two effects are additive with regard to level of constitutivity, but only that portion of synthesis attributable to the dsdC mutation is cyclic AMP dependent.
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis / Q000494:pharmacology; D000242:Cyclic AMP / Q000494:pharmacology*; D004790:Enzyme Induction; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D006150:Guanine Nucleotides / Q000494:pharmacology*; D006159:Guanosine Tetraphosphate / Q000494:pharmacology*; D012695:L-Serine Dehydratase / Q000096:biosynthesis*; D008954:Models, Biological; D009154:Mutation; D012694:Serine / Q000494:pharmacology*
chemical_list: D001426:Bacterial Proteins; D006150:Guanine Nucleotides; D006159:Guanosine Tetraphosphate; D012694:Serine; D000242:Cyclic AMP; D012695:L-Serine Dehydratase