Relationship between the methionine tryptic peptides of simian virus 40 and BK virus tumor antigens.
The monomer form of BK virus (BKV) tumor antigen (T Ag) was immunoprecipitated from extracts of BKV-transformed cells and had a molecular weight of approximately 113,000. This compared with 97,000 for the molecular weight of either BKV or simian virus 40 (SV40) T Ag from lytically infected cells. The SV40 and BKV T Ag's from productively infected cells were compared by examining their methionine-labeled tryptic peptides. Out of a total of 20 SV40-and 21 BKV-specific peptides, there were seven pairs of similar peptides on the basis of ion-exchange chromatography, These coeluting peptides contained approximately 25 to 30% of the total methionine radioactivity. Similar results were obtained when the tryptic peptides of SV40 T Ag from lytically infected cells were compared with those of BKV T Ag from virally transformed cells.
D000951:Antigens, Neoplasm / Q000032:analysis*; D000956:Antigens, Viral / Q000032:analysis*; D001739:BK Virus / Q000276:immunology*; D002460:Cell Line; D002472:Cell Transformation, Viral; D008715:Methionine / Q000032:analysis; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis*; D011120:Polyomavirus / Q000276:immunology*; D011487:Protein Conformation; D013539:Simian virus 40 / Q000276:immunology*
D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D010455:Peptides; D008715:Methionine