title: [Rational method of obtaining sera with a high titre of virus-neutralizing antibodies. Report 2].
abstract: In addition to report I (ZHMEI, 1977, No. 1) a study was made of 9 more schemes of rabbit immunization with the poliomyelitis virus, type I, for the purpose of obtaining the neutralizing sera of high titre. Vitamins A and C were used in the experiments in the capacity of the activators of the organism reaction; Freund's adjuvant of different make was tested; different reimmunization periods and different amounts of the adjuvant were administered. Titration of rabbit sera in the process of immunization and reimmunization showed immunization into the lymph nodes with the subsequent single reimmunization in one month to be the most effective and economical method of obtaining high effective sera.
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000302:isolation & purification*; D005620:Freund's Adjuvant / Q000008:administration & dosage; D007106:Immune Sera; D007272:Injections, Intralymphatic; D009500:Neutralization Tests; D017955:Poliovirus / Q000276:immunology*; D011054:Poliovirus Vaccine, Inactivated / Q000008:administration & dosage; D011817:Rabbits; D012699:Serology / Q000379:methods*; D014801:Vitamin A / Q000008:administration & dosage
chemical_list: D000914:Antibodies, Viral; D007106:Immune Sera; D011054:Poliovirus Vaccine, Inactivated; D014801:Vitamin A; D005620:Freund's Adjuvant
vernacular_title: Ratsional'nyĭ metod polucheniia syvorotok s vysokim titrom virusneĭtralizuiushchikh antitel. Soobshchenie 2.