Regulation of glycogen synthetase and phosphorylase phosphatase activities in rat adipose tissue.
Incubation of a rat adipose tissue homogenate causes a time and temperature dependent activation of glycogen synthetase (UDP glucose:glycogen 4-alpha-glucosyltransferase) and simultaneous inactivation of phosphorylase (1,4-alpha-D-glucan: orthophosphate alpha-glucosyltransferase, EC 2.4.1.1). Activation of glycogen synthetase at 15 and 23 degrees C was preceded by a lag period. The duration of the lag period could not be correlated with significant changes in phosphorylase activity. Addition of glucose and methylxanthines caused an increase in the rates of glycogen synthetase activation and phosphorylase inactivation. The effect on glycogen synthetase activation was mainly on the linear phase. Addition of AMP inhibited phosphorylase inactivation and accelerated glycogen synthetase activation. Addition of muscle phosphorylase alpha caused a prolongation of the lag period which lasted until phosphorylase alpha activity had decreased to the level originally present in the preparation. It is concluded that in adipose tissue activation of glycogen synthetase is not dependent on prior inactivation of phosphorylase and that other factors should be looked for to explain the lag period preceding glycogen synthetase activation.
D000249:Adenosine Monophosphate / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000273:Adipose Tissue / Q000201:enzymology*; D000818:Animals; D002110:Caffeine / Q000494:pharmacology; D004789:Enzyme Activation; D005947:Glucose / Q000494:pharmacology; D006006:Glycogen Synthase / Q000378:metabolism*; D008297:Male; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D010765:Phosphorylase Phosphatase / Q000378:metabolism*; D051381:Rats; D013696:Temperature; D014970:Xanthines / Q000494:pharmacology
D014970:Xanthines; D002110:Caffeine; D000249:Adenosine Monophosphate; D000255:Adenosine Triphosphate; D006006:Glycogen Synthase; D010765:Phosphorylase Phosphatase; D010744:Phosphoric Monoester Hydrolases; D005947:Glucose