title: Transient virus expression during murine leukemia induction by X-irradiation.
abstract: Most X-irradiation-induced thymomas in C57BL/6 mice are virus-free when assayed by immunofluorescence for the gs antigen (gsa) of murine leukemia virus (MuLV). Virus was induced transiently in bone marrow cells and later appeared in thymus cells. Six to 7 weeks post irradiation, thymocytes and bone marrow cells were MuLV gsa-negative and remained negative for the lifetime of most animals, whether or not they contracted overt leukemia. During the period when MuLV gsa-positive bone marrow cells were found, XC-positive syncytia-producing bone marrow cells were also found. Virus information was expressed, therefore, for a limited duration, long before any signs of leukemia in the animals were evident. MuLV gsa-positive  thymocytes taken from mice 4 weeks after X-irradiation were cocultivated with a series of indicator cells. B-tropic virus, in addition to a xenotropic virus, was isolated from these cells. Ecotropic virus was not found in normal mouse thymocytes, in irradiated thymocytes a few days after termination of the X-irradiation sequence, or in most primary thymomas. All thymocytes produced only xenotropic virus in the cocultivation assays. Expression of the ecotropic virus was, therefore, transient, as assayed by immunofluorescence, XC syncytia formation, and virus isolation from MuLV gsa-positive thymus cells.
mesh_terms: D000818:Animals; D000956:Antigens, Viral; D001853:Bone Marrow / Q000276:immunology / Q000382:microbiology; D001854:Bone Marrow Cells; D002462:Cell Membrane / Q000276:immunology; D005260:Female; D009052:Leukemia Virus, Murine / Q000276:immunology / Q000302:isolation & purification* / Q000528:radiation effects; D007942:Leukemia, Experimental / Q000276:immunology / Q000382:microbiology; D007953:Leukemia, Radiation-Induced / Q000276:immunology / Q000382:microbiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D013601:T-Lymphocytes / Q000276:immunology / Q000382:microbiology; D013997:Time Factors; D014779:Virus Replication / Q000528:radiation effects; D014965:X-Rays
chemical_list: D000956:Antigens, Viral