Purification of mengovirus by freon extraction and chromatography on protein-coated controlled pore glass.
Mengovirus, extracted from infected L-cell cultures with Freon 113 and concentrated from the aqueous phase with polyethyleneglycol, was chromatographed on protein-coated controlled pore glass (CPG). The covalent binding of protein to CPG is described. Further purification and concentration of mengovirus was achieved by isopycnic density gradient centrifugation in solutions of either CsCl or salts of iodinated benzoic acid derivatives. The described procedure is superior to conventional methods for the isolation and purification of large quantities of mengovirus. It yields highly purified virus preparations within a short time and a recovery of more than 50% of the starting infectivity.
D011232:Chemical Precipitation; D005617:Chlorofluorocarbons, Methane; D002845:Chromatography / Q000379:methods*; D007739:L Cells; D008574:Mengovirus / Q000302:isolation & purification*; D011092:Polyethylene Glycols
D005617:Chlorofluorocarbons, Methane; D011092:Polyethylene Glycols