Binding of the endogenous nonpeptide morphine-like compound to opiate receptors.
The endogenous central nervous tissue substance called MLC (morphine-like compound) is shown to bind to the opiate receptors of the mouse neuroblastoma X glioma hybrid cell line NG108-15. The interaction of MLC with these opiate receptors is noncooperative, as is the interaction of morphine, naloxone, and Leu-enkephalin with these receptors. A specific antibody to morphine will bind MLC but will not bind beta-endorphin, Leu-enkephalin, or Met-enkephalin. It would appear, therefore, that MLC can be considered to be a different type of endogenous ligand for the opiate receptor.
D000818:Animals; D001667:Binding, Competitive; D001923:Brain Chemistry; D002417:Cattle; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D004745:Enkephalins / Q000378:metabolism; D005048:Etorphine / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D009020:Morphine / Q000378:metabolism; D009270:Naloxone / Q000378:metabolism; D009294:Narcotics / Q000302:isolation & purification / Q000378:metabolism*; D011863:Radioimmunoassay; D011957:Receptors, Opioid / Q000378:metabolism*
D004745:Enkephalins; D009294:Narcotics; D011957:Receptors, Opioid; D009270:Naloxone; D005048:Etorphine; D009020:Morphine