Lateral enzyme topology in the rough endoplasmic reticulum of rat liver.
Rough microsomes were subfractionated on the basis of different properties in order to investigate the nature and extent of the enzyme heterogeneity of these vesicles. A discontinuous gradient, containing monovalent cations allowed the separation of a ribosome-poor membrane fraction which was enriched in electron transport enzymes and relatively poor in phosphatases. Zonal centrifugation on a stabilizing gradient separated 3 fractions characterized by enrichment of electron transport enzymes, glucose-6-phosphatase and adenosinetriphosphatase, respectively. An essentially similar pattern was seen when ribosomes were removed with EDTA and the denuded vesicles subfractionated on a sucrose gradient. Rough microsomes from phenobarbital-treated rats exhibited the same pattern both qualitatively and quantitatively. It appears that electron transport enzymes and two types of phosphatases are heterogeneously distributed among rough microsomal vesicles.
D000818:Animals; D002458:Cell Fractionation; D002499:Centrifugation, Density Gradient; D002501:Centrifugation, Zonal; D003579:Cytochrome Reductases / Q000032:analysis; D004492:Edetic Acid; D008055:Lipids / Q000032:analysis; D008099:Liver / Q000201:enzymology; D008297:Male; D008851:Micropore Filters; D008862:Microsomes, Liver / Q000032:analysis / Q000201:enzymology*; D010634:Phenobarbital / Q000494:pharmacology; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis; D011506:Proteins / Q000032:analysis; D051381:Rats; D011952:Receptors, Concanavalin A / Q000032:analysis; D012270:Ribosomes / Q000201:enzymology
D008055:Lipids; D011506:Proteins; D011952:Receptors, Concanavalin A; D004492:Edetic Acid; D003579:Cytochrome Reductases; D010744:Phosphoric Monoester Hydrolases; D010634:Phenobarbital