Characterization of simian virus 40 transformed African green monkey cells (CV-1). I. Defective virion and viral genome.
Several clones of SV40 transformed CV-1 cells have been characterized for the production of T- and V-antigens and for the state of viral genome. The transformed CV-1 cells failed to produce infectious virions as assayed after sonication or cocultivation and fusion with normal CV-1 cells, and were resistant to super-infection by SV40. Some clones of the transformed cells contained V-antigens. The population of V-antigen positive cells varied from 0 to 100% depending on the passage number while the T-antigen positive cells were always 100%. The virions isolated from the transformed cells were similar in morphology to complete SV40, but lighter in density than complete SV40. In one clone, a small amount of SV40 DNA was detectable in a free state while a large proportion of the DNA hybridizable with SV40 3H cRNA was linearly integrated into the cell DNA. The free SV40 DNA was noninfectious, closed circular DNA with a size smaller than infectious SV40 DNA component I. Since the cell extracts of the transformed cells contained an agent(s) which induced T- and V-antigens in normal CV-1 cells, it was suggested that the SV40 transformed CV-1 cells contained free as well as integrated defective SV40 genomes responsible for the synthesis of T- and V-antigens.
D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis; D002459:Cell Fusion; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D004279:DNA, Viral / Q000032:analysis; D003673:Defective Viruses / Q000032:analysis / Q000254:growth & development / Q000276:immunology; D000882:Haplorhini; D013539:Simian virus 40 / Q000032:analysis / Q000254:growth & development / Q000276:immunology
D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D004279:DNA, Viral