[Biological method of quantitating vitamin D in different preparations and diets].
A biological method for quantitative assay of vitamin D3 in various preparations has been developed. The method is based on a close correlation between the vitamin D3 dose and the content of calcium-binding protein in the duodenal mucosa of rachitic chickens 72 hrs after vitamin D3 administration. Calcium-binding protein is assayed by radial immunodiffusion in agar-agar. The calibration curve of the dose-protein dependence allows measurements of I to 500 IU of vitamin D3 in the sample tested. The method shows good accuracy and reproducibility. The paper presents the results of quantitation of vitamin D in various preparations.
D000818:Animals; D001681:Biological Assay; D002118:Calcium / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism; D002645:Chickens; D002762:Cholecalciferol / Q000032:analysis*; D004386:Duodenum / Q000378:metabolism; D005504:Food Analysis; D007413:Intestinal Mucosa / Q000378:metabolism; D011863:Radioimmunoassay / Q000379:methods
D002352:Carrier Proteins; D002762:Cholecalciferol; D002118:Calcium
Biologicheskiĭ metod kolichestvennogo opredeleniia bitamina D v razlichnykh preparatakh i kormakh.