Semisynthetic cytochrome c.
Horse heart cytochrome c can be split with cyanogen bromide into a heme peptide (residues 1-65) and a nonheme peptide (residues 66-104). In a process involving (i) complex formation between the two fragments and (ii) restoration of the severed peptide linkage, a fully active cytochrome c preparation can be re-formed. Use has been made of this process to couple the heme peptide to peptide 66-104 synthesized by the Merrifield solid-phase procedure. The semisynthetic product formed in this manner is indistinguishable from reconstituted cytochrome c prepared with nonsynthetic peptide 66-104.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D003574:Cytochrome c Group / Q000032:analysis / Q000138:chemical synthesis*; D006736:Horses; D009206:Myocardium / Q000201:enzymology; D010455:Peptides / Q000032:analysis / Q000138:chemical synthesis; D011863:Radioimmunoassay
D000596:Amino Acids; D003574:Cytochrome c Group; D010455:Peptides