Replication of Mouse Sarcoma Virus (Moloney) and its helper in a human heteroploid cell line of malignant origin.
A human heteroploid cell line of malignant origin (J111) was infected with Mouse Moloney Sarcoma Virus. [MSV-M(MLV)]. The replication of the virus was demonstrated by different methods. After a few passages of infected cultures, the transforming component of MSV-M(MLV) disappeared from the supernatant fluids and cells. The non transforming helper component continued to be produced. The relatively low amount of helper virus released by the cells suggests a control of its replication in the J111 cellular system.
D000782:Aneuploidy; D002460:Cell Line; D002472:Cell Transformation, Viral; D006378:Helper Viruses; D006801:Humans; D007951:Leukemia, Myeloid; D008854:Microscopy, Electron; D008979:Moloney murine leukemia virus; D012190:Retroviridae / Q000648:ultrastructure; D014776:Virus Cultivation / Q000379:methods; D014779:Virus Replication