Cytochrome c oxidase activity in T/t6 (balanced lethal) mutant mice.
R-1 (1450g) and R-2 (25,000g) liver fractions from T/t6 and B6CBAF1 hybrid mice were analyzed for their protein content, mitochondria concentrations, and activities of three respiratory-chain enzymes of the mitochondrial inner membrane: cytochrome c oxidase (ferrocytochrome c: oxygen oxidoreductase, E.C. 1.9.3.1.), alpha-glycerophosphate dehydrogenase [L-glycerol-3-phosphate: (acceptor) oxidoreductase, E.C. 1.1.99.5], and succinate-cytochrome c reductase. Only cytochrome c oxidase activity, calculated as units per 10(10) mitochondria, was significantly lower in both R-1 and R-2 fractions of T/t6 mice. Cytochrome c oxidase activity varied greatly among T/t6 mice, as did their liver mitochondria concentrations and body weights. Cytochrome c oxidase activity in the R-1 fraction of T/t6 mice, averaged about 40% lower than in B6CBAF1 mice. alpha-Glycerophosphate dehydrogenase activity was often elevated in T/t6 mice, particularly in the R-2 fraction. The T/t locus, a complex genetic locus on chromosome 17, may contain genes important to the function and biogenesis of mitochondria.
D000367:Age Factors; D000818:Animals; D001835:Body Weight; D003576:Electron Transport Complex IV / Q000378:metabolism*; D005804:Genes, Lethal; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism*; D006824:Hybridization, Genetic; D008099:Liver / Q000201:enzymology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008928:Mitochondria / Q000201:enzymology*; D010088:Oxidoreductases / Q000378:metabolism*; D013384:Succinate Cytochrome c Oxidoreductase / Q000378:metabolism*
D010088:Oxidoreductases; D013384:Succinate Cytochrome c Oxidoreductase; D005993:Glycerolphosphate Dehydrogenase; D003576:Electron Transport Complex IV