Indirect microhemagglutination test for varicella--zoster antibody determination.
An indirect microhemagglutination assay (IHA) was devised because of a need to provide an alternative test to complement fixation (CF) for varicella-zoster (V-Z) antibody determination. Human erythrocytes were sequentially treated with 2% glutaraldehyde, 0.04% tannic acid, and 2% pyruvic aldehyde then exposed to sonicated V-Z infected cells. This same tanning procedure was suitable for herpes simplex and Epstein-Barr virus antigen attachment but unsatisfactory for several non-herpes-group viruses. V-Z antibody titres determined by IHA were generally 2 to 6 times higher than CF titres. Cross-reaction with herpes simplex antibody was minimal.
D000328:Adult; D000914:Antibodies, Viral / Q000032:analysis*; D002648:Child; D003429:Cross Reactions; D004912:Erythrocytes / Q000187:drug effects; D005069:Evaluation Studies as Topic; D005455:Fluorescent Antibody Technique; D005976:Glutaral / Q000494:pharmacology; D006386:Hemagglutination Tests; D014645:Herpesvirus 3, Human / Q000276:immunology*; D006801:Humans; D047348:Hydrolyzable Tannins / Q000494:pharmacology; D011765:Pyruvaldehyde / Q000494:pharmacology; D018139:Simplexvirus / Q000276:immunology
D000914:Antibodies, Viral; D047348:Hydrolyzable Tannins; D011765:Pyruvaldehyde; D005976:Glutaral