[Morphological (Macro-, micro-electronoptical) changes and cytoenzyme changes after ligation of the pulmonary artery, pulmonary veins and both in dogs].
In six dogs ligature of the main pulmonary artery was performed, and in five other ligature of the pulmonary veins was carried out, while in five other animals combined simultaneous ligatures were made. Extensive infarcization was noted, of the pulmonary tissue, with an evolution toward death, in all types of ligatures. Death occured earlier in ligatures of the pulmonary veins. All the interventions were performed on the left lung, under general anesthesia, orotracheal intubation and assisted respiration. Electron-optic investigation revealed mitochondrial alterations 30 minutes after ligature of the pulmonary veins and 2 hours after that of the pulmonary arteries. The lesions were extensive in time. In the infarction areas the activity of the energetic enzymes (SDH, NADH2, G6PD, LDM) appears to be reduced, and even completly abolished. This occurs earlier when pulmonary veins are closed. On the basis of clinical and surgical data already mentioned in the literature, as well as on that of the experimental studies performed, the authors consider that ligature of the pulmonary artery, either therapeutically or due to various necessities, an intervention that is recommended by some surgeons, is not a functional type of intervention, and can even have ill-fated results, especially if the pulmonary venous circulation is also involve in the pathological process.
D000818:Animals; D004285:Dogs; D003576:Electron Transport Complex IV / Q000378:metabolism*; D008026:Ligation; D008168:Lung / Q000201:enzymology / Q000473:pathology / Q000648:ultrastructure; D008171:Lung Diseases / Q000209:etiology*; D010088:Oxidoreductases / Q000378:metabolism*; D011183:Postoperative Complications; D011651:Pulmonary Artery / Q000601:surgery*; D011667:Pulmonary Veins / Q000601:surgery*
D010088:Oxidoreductases; D003576:Electron Transport Complex IV
Modificări morfologice (macro-, microelectronooptice) şi citoenzimatice după ligatura la cîine a arterei pulmonare, a venelor pulmonare şi mixtă.