Synthesis and template-directed polymerization of adenylyl(3'-5')adenosine cyclic 2', 3'-phosphate.
Adenylyl(3'-5')adenosine cyclic 2',3'-phosphate (A-A greater than p) was synthesized and its polymerization was attempted under various conditions inthe presence of poly(uridylic acid) and1,3-propanediamine. Reaction at -20 degrees C for 16 days gave polymerized products (up to the 8-mer) in 15% yield and was proved to be dependent on the template. Reaction at 0 degrees C for 16 days gave more extensive (up to the 10-mer) and more efficient (35%) polymerization. The newly formed phosphodiester linkage was exclusively 2'-5'. These results are discussed in comparison with the monomer-condensation reaction.
D000249:Adenosine Monophosphate; D000242:Cyclic AMP; D007700:Kinetics; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides; D011119:Polynucleotides / Q000138:chemical synthesis*; D013696:Temperature; D013698:Templates, Genetic
D009841:Oligonucleotides; D011119:Polynucleotides; D000249:Adenosine Monophosphate; D000242:Cyclic AMP