title: Recent excitement in the DNA replication problem.
abstract: It is now possible to reproduce most of the reactions involved in DNA replication using prokaryotic enzymes in vitro. Such systems have revealed that DNA replication is a complex process depending on a relatively large number of proteins, and that nucleoside triphosphate hydrolysis energy is used at several discrete steps. Much of the complexity of DNA replication may arise from the need for extreme copying fidelity.
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D003090:Coliphages / Q000235:genetics; D004265:DNA Helicases / Q000378:metabolism; D004261:DNA Replication; D004269:DNA, Bacterial / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D009154:Mutation; D009690:Nucleic Acid Conformation
chemical_list: D004269:DNA, Bacterial; D004279:DNA, Viral; D000255:Adenosine Triphosphate; D004259:DNA-Directed DNA Polymerase; D004265:DNA Helicases