Purification of hepatitis A virus from human feces.
Hepatitis A virus was purified fecal specimens obtained from 2 patients with naturally acquired hepatitis A. The purification procedure involved differential centrifugation, organic solvent extraction, agarose gel filtration, ion-exchange chromatography, and isopycnic ultracentrifugation in cesium chloride. Using immune electron microscopy and discontinuous SDS-PAGE, this procedure was found to be effective in removing extraneous material from hepatitis A virus. There was significant recovery of virus as judged by immune electron microscopy and solid-phase radioimmunoassay. Using this protocol, it has been possible to obtain virus preparations of sufficient purity and high enough titer to enable biochemical studies to proceed.
D000937:Antigen-Antibody Reactions; D002500:Centrifugation, Isopycnic; D002847:Chromatography, Agarose; D002852:Chromatography, Ion Exchange; D005243:Feces / Q000382:microbiology*; D006506:Hepatitis A / Q000382:microbiology; D006507:Hepatovirus / Q000302:isolation & purification*; D006801:Humans; D008854:Microscopy, Electron; D014764:Viral Proteins / Q000032:analysis
D014764:Viral Proteins