Binding assay for opioid peptides with neuroblastoma x glioma hybrid cells: specificity of the receptor site.
A sensitive and rapid radioreceptor assay has been developed to monitor opioid peptides in column effluents. It is based on competitive binding to NG 108-15 cells using [3H-Tyr]Leu-enkephalin as the displaced ligand. The specificity of binding to the cells of naturally occurring opioid peptides and synthetic analogs has been shown to be similar to that found with synaptic plasma membranes.
D000818:Animals; D001667:Binding, Competitive; D002460:Cell Line; D004723:Endorphins / Q000378:metabolism; D004745:Enkephalins / Q000378:metabolism; D005910:Glioma / Q000378:metabolism*; D051379:Mice; D009374:Neoplasms, Experimental / Q000378:metabolism; D009447:Neuroblastoma / Q000378:metabolism*; D011869:Radioligand Assay / Q000379:methods*; D011957:Receptors, Opioid / Q000378:metabolism*; D008083:beta-Lipotropin / Q000378:metabolism
D004723:Endorphins; D004745:Enkephalins; D011957:Receptors, Opioid; D008083:beta-Lipotropin