title: Cell cycle dependency of murine cytomegalovirus replication in synchronized 3T3 cells.
abstract: Synchronized murine 3T3 cells have been used to investigate the possible dependency of murine cytomegalovirus replication upon the cell cycle. The normal latent period of 12 h characteristic of asynchronous 3T3 cells was protracted to more than 24 h after an early G1 infection in synchronous cells. In this case viral progeny were not detected until after the initiation of the host S-phase. Cells maintained in the G1 phase did not replicate virus. This failure could not be explained by a decrease in virus penetration but was apparently due to a requirement for an event associated with the host S-phase. Thymidine-induced inhibition of cell cycle traverse also blocked virus replication. Viral DNA synthesis did not initiate until after the initiation of host DNA. In contrast, herpes simplex virus type 1 replicated in 3T3 cells independently of the cell cycle.
mesh_terms: D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D003584:Cytological Techniques; D003587:Cytomegalovirus / Q000254:growth & development* / Q000378:metabolism; D004247:DNA / Q000096:biosynthesis; D004279:DNA, Viral / Q000096:biosynthesis; D051379:Mice; D013936:Thymidine / Q000378:metabolism; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D004247:DNA; D013936:Thymidine