[Lipoproteins as serum inhibitors in rubella hemagglutination inhibition test and their elimination (author's transl)].
Lipoproteins of 20 human sera were isolated by flotation centrifugation and tested for inhibitory activity in rubella HI-test. Low density lipoproteins (LDL) showed the highest titers with an average of 1:238,0, whereas the others (VLDL, HDL) had lower titers (see tab. 2). The greatest reduction of all classes of lipoproteins was achieved with kaolin treatment. Heparin-manganous chloride and dextran sulfate-calcium chloride method mainly reduced the LDL (84.4 resp. 78.7%). In a comparative test sera treated with the heparin procedure showed higher antibody titers than those treated with dextran sulfate.
D000328:Adult; D000914:Antibodies, Viral / Q000302:isolation & purification; D000917:Antibody Formation; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D008074:Lipoproteins / Q000097:blood*; D008075:Lipoproteins, HDL / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood; D008079:Lipoproteins, VLDL / Q000097:blood; D012409:Rubella / Q000097:blood / Q000276:immunology*
D000914:Antibodies, Viral; D008074:Lipoproteins; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL
Lipoproteine als Seruminhibitoren beim Röteln- Hämagglutinationshemmungstest und deren Eliminierung.