Persistence of immunoglobulin and complement components C4 and C3 bound to guinea pig tumor cells.
Guinea pig hepatoma cells (line-10) growing as ascites were studied for the presence of immunoglobulin, C4, and C3 (components of complement) on their surfaces. Immunoglobulin, C4, and C3 content increased with length of time spent in the peritoneal cavity. The persistence of these factors bound in vivo or in vitro was also determined. Complement-fixing (CF) activity of cellbound antibody disappeared from the surface more rapidly at 37 degrees C than at 4 degrees C; the continued presence of cellbound immunoglobulin in non-complement-fixing form could be demonostrated at either temperature. No CF activity was released into the medium. C4 and C3 were released into the medium and could be demonstrated in the medium by immunochemical methods.
D000818:Animals; D000912:Antibodies, Neoplasm / Q000032:analysis; D000963:Antimetabolites / Q000494:pharmacology; D006528:Carcinoma, Hepatocellular / Q000276:immunology*; D002455:Cell Division; D002462:Cell Membrane / Q000276:immunology; D003176:Complement C3; D003181:Complement C4; D003165:Complement System Proteins; D006168:Guinea Pigs; D007136:Immunoglobulins; D066298:In Vitro Techniques; D008113:Liver Neoplasms / Q000276:immunology*; D013696:Temperature; D013997:Time Factors
D000912:Antibodies, Neoplasm; D000963:Antimetabolites; D003176:Complement C3; D003181:Complement C4; D007136:Immunoglobulins; D003165:Complement System Proteins