Introduction of the herpes simplex virus thymidine kinase gene into mouse cells using virus DNA or transformed cell DNA.
Cells lacking the enzyme thymidine kinase (LMTK- cells) have been transformed to a kinase-positive phenotype using sheared herpes simplex virus (HSV) DNA, and the enzyme found in these transformed cells is HSV-specific. One of the cell lines is able to complement the functional defect found in two temperature-sensitive mutants of HSV 1, and reversion of the cells to a thymidine kinase-negative phenotype results in the loss of this capability. The HSV thymidine kinase gene can also be introduced into LMTK- cells using DNA extracted from transformed cells, and the high efficiency of this procedure suggests that the state of the virus DNA in transformed cells is different from that of DNA in virus particles.
D002460:Cell Line; D002472:Cell Transformation, Viral; D004247:DNA / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D005814:Genes, Viral; D009154:Mutation; D018139:Simplexvirus / Q000235:genetics*; D013937:Thymidine Kinase / Q000096:biosynthesis*; D014162:Transfection
D004279:DNA, Viral; D004247:DNA; D013937:Thymidine Kinase