Isolation and characterization of immunoregulatory factors from normal human serum. I. Preliminary biochemical and biological characterization of immunosuppressive factors.
Normal human serum was shown to inhibit the mitogenic effects of bacterial lipopolysaccharide and Con A on mouse spleen lymphocytes and reduce the in vitro antibody response to SRBC by these cells. Furthermore, it was demonstrated that immune suppression occurred without loss of lymphocyte viability. Fractionation of normal human serum resulted in isolation of several immunoenhancing and immunoinhibitory fractions. Electrophoretic analysis of the immunoinhibitory fractions revealed a complex array of serum proteins. The most prominent proteins on polyacrylamide electrophoresis stained for both proteins and carbohydrate. The heterogeneity of immunoinhibitory fractions were further substantiated by their differential susceptibility to trypsin, periodate, and 2-mercaptoethanol treatment. Heterogeneity of the fractions was also shown to be related to difference in their biologic activity as expressed in their effects on mitogenicity and immunogenicity of LPS in mouse splenic cultures. This study lends evidence to the consideration that normal human serum contains several immunoregulatory factors with differing biochemical characteristics and cellular sites of action.
D000818:Animals; D000917:Antibody Formation; D002470:Cell Survival; D002852:Chromatography, Ion Exchange; D003208:Concanavalin A / Q000494:pharmacology; D004306:Dose-Response Relationship, Immunologic; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007122:Immunoelectrophoresis; D007166:Immunosuppressive Agents / Q000032:analysis* / Q000097:blood / Q000302:isolation & purification; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation; D008214:Lymphocytes; D008623:Mercaptoethanol / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D010504:Periodic Acid / Q000494:pharmacology; D014357:Trypsin / Q000494:pharmacology
D007166:Immunosuppressive Agents; D008070:Lipopolysaccharides; D010504:Periodic Acid; D003208:Concanavalin A; D008623:Mercaptoethanol; D014357:Trypsin