Actions of peptides isolated from amphibian skin on pancreatic acinar cells.
In dispersed acinar cells prepared from guinea pig pancreas, peptides isolated from amphibian skin (caerulein, bombesin, litorin, and physalaemin) as well as eledoisin, a peptide isolated from the posterior salivary gland of a Mediterranean octopod, increased outflux of 45Ca, release of bound 45Ca, accumulation of cyclic GMP, and release of amylase. In addition, bombesin, litorin, physalaemin, and eledoisin each increased the initial uptake of 45Ca by dispersed acinar cells, whereas C-terminal octapeptide of porcine cholecystokinin (CCK-OP) and carbamylcholine did not increase the initial uptake of 45Ca but, rather, abolished the increase caused by the other agents. None of the actions of these amphibian peptides was altered by concentrations of atropine sufficient to abolish the effects of muscarinic cholinergic agents. None of the amphibian peptides altered cellular cyclic AMP or the increase caused by secretin or porcine vasoactive intestinal peptide (VIP). Acinar cells preincubated with 45Ca plus bombesin showed the same rate of release of 45Ca as did control cells and this rate was not altered by adding bombesin but was increased fivefold by adding CCK-OP. In terms of their chemical structures as well as the potency and efficacy with which they alter acinar cell function, the amphibian peptides plus CCK-OP can be grouped into three pairs: caerulein with CCK-OP, bombesin with litorin, and physalaemin with eledoisin.
D000681:Amylases / Q000378:metabolism; D000818:Animals; D001001:Anura; D001693:Biological Transport, Active / Q000187:drug effects; D001839:Bombesin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002108:Ceruletide / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism; D004600:Eledoisin / Q000494:pharmacology; D006168:Guinea Pigs; D009842:Oligopeptides / Q000494:pharmacology*; D010179:Pancreas / Q000378:metabolism*; D010803:Physalaemin / Q000494:pharmacology; D011506:Proteins / Q000494:pharmacology
D009842:Oligopeptides; D011506:Proteins; D010803:Physalaemin; D004600:Eledoisin; D002108:Ceruletide; D000242:Cyclic AMP; D000681:Amylases; D006152:Cyclic GMP; D001839:Bombesin; D002118:Calcium