Cyclic adenosine 3',5'-monophosphate levels and activities of adenylate cyclase and cyclic adenosine 3',5'-monophosphate phosphodiesterase in Pseudomonas and Bacteroides.
A modified Gilman assay was used to determine the concentrations of cyclic adenosine 3',5'-monophosphate (cAMP) in rapidly filtered cells and in the culture filtrates of Pseudomonas aeruginosa, Escherichia coli K-12, and Bacteroides fragilis. In P. aeruginosa cultures, levels of cAMP in the filtrate increased with the culture absorbance (3.5 to 19.8 X 10(-9) M) but did not vary significantly with the carbon source used to support growth. Intracellular concentrations (0.8 to 3.2 X 10(-5) M) were substantially higher and did not vary appreciably during growth or with carbon source. Sodium cAMP (5 mM) failed to reverse the catabolite repression of inducible glucose-6-phosphate dehydrogenase (EC 1.1.1.49) synthesis caused by the addition of 10 mM succinate. Exogenous cAMP also had no discernible effect on the catabolite repression control of inducible mannitol dehydrogenase (EC 1.1.1.67). P. aeruginosa was found to contain both soluble cAMP phosphodiesterase (EC 3.1.4.17) and membrane-associated adenylate cyclase (EC 4.6.1.1) activity, and these were compared to the activities detected in crude extracts of E. coli. B. fragilis crude cell extracts contain neither of these enzyme activities, and little or no cAMP was detected in cells or culture filtrates of this anaerobic bacterium.
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000378:metabolism*; D000262:Adenylyl Cyclases / Q000378:metabolism*; D000446:Aldehyde-Lyases / Q000096:biosynthesis; D001441:Bacteroides fragilis / Q000201:enzymology / Q000378:metabolism*; D000242:Cyclic AMP / Q000378:metabolism* / Q000494:pharmacology; D004794:Enzyme Repression; D004926:Escherichia coli / Q000378:metabolism; D005954:Glucosephosphate Dehydrogenase / Q000096:biosynthesis; D008354:Mannitol Dehydrogenases / Q000096:biosynthesis; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000201:enzymology / Q000378:metabolism*; D013386:Succinates / Q000494:pharmacology
D013386:Succinates; D000242:Cyclic AMP; D008354:Mannitol Dehydrogenases; D005954:Glucosephosphate Dehydrogenase; D010727:Phosphoric Diester Hydrolases; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D000446:Aldehyde-Lyases; D000262:Adenylyl Cyclases