[Regulation of hepatocyte 3-hydroxy-3-methylglutaryl coenzyme A reductase in primary cell cultures through low density lipoproteins].
The regulation of hepatic cholesterol biosynthesis by low density lipoprotein (LDL) was studied by using a new cell culture method of isolated rat hepatocytes. After two days in culture no supressant effect of LDL on 3-hydroxy-3-methylglutaryl coenzyme A reductase activity (HMG-CoA) was detected. The primary culture of rat hepatocytes lead to the growth of a cell monolayer with stable liver specific properties during 4 days of culture. LDL did suppress HMG-CoA-reductase activity of rat hepatocytes in primary culture 5 days after seeding, when dedifferentiation has started.
D000818:Animals; D002478:Cells, Cultured; D002784:Cholesterol / Q000096:biosynthesis*; D003864:Depression, Chemical; D004791:Enzyme Inhibitors; D006903:Hydroxymethylglutaryl CoA Reductases / Q000378:metabolism*; D066298:In Vitro Techniques; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D051381:Rats
D004791:Enzyme Inhibitors; D008077:Lipoproteins, LDL; D002784:Cholesterol; D006903:Hydroxymethylglutaryl CoA Reductases
Die Regulation der 3-Hydroxy-3-Methylglutaryl-Coenzym-A-Reduktase von Hepatozyten in primärer Zellkultur durch Low Density Lipoprotein.