Synthesis of Sendai virus polypeptides by a cell-free extract from wheat germ.
The '18S' RNA population from Sendai virus-infected cells efficiently directed the synthesis of three virus-specific polypeptides P, NP and M, in wheat germ cell-free extracts. In agreement with previous results obtained with a reticulocyte extract, there was little or no production of virus glycopolypeptides. Analyses of tryptic peptides revealed close correspondence between the primary structures of NP and M made in vitro and the authentic virus polypeptides.
D002474:Cell-Free System; D006020:Glycopeptides / Q000096:biosynthesis; D007930:Leucine / Q000378:metabolism; D009029:Mosaic Viruses / Q000378:metabolism; D010222:Parainfluenza Virus 1, Human / Q000378:metabolism*; D010452:Peptide Biosynthesis; D010936:Plant Extracts; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism; D014908:Triticum; D014764:Viral Proteins / Q000096:biosynthesis*
D006020:Glycopeptides; D010936:Plant Extracts; D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; D007930:Leucine