Purification and properties of bovine liver lysosomal adenosine 5'-phosphosulphate sulphohydrolase. A non-specific enzyme with pyrophosphatase and phosphodiesterase activities.
Bovine liver lysosomal adenosine 5'-phosphosulphate sulphohydrolase (EC 3.6.2.1) was purified to apparent homogeneity. The molecular weight of the enzyme was 53 000 by sodium dodecyl sulphate polyacrylamide gel electrophoresis and 56 000 by BioGel P-150 gel filtration. The substrate specificity of the enzyme was studied. The several substrates towards which the enzyme preparation showed high activity were used to establish that a single enzyme was responsible for the different activities. This multiple specificity provides a possible explanation of the physiological role of lysosomal adenosine 5'-phosphosulphate sulphohydrolase.
D000250:Adenosine Phosphosulfate; D000818:Animals; D001667:Binding, Competitive; D002417:Cattle; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008247:Lysosomes / Q000201:enzymology*; D010727:Phosphoric Diester Hydrolases / Q000302:isolation & purification / Q000378:metabolism*; D011755:Pyrophosphatases / Q000302:isolation & purification / Q000378:metabolism*; D013379:Substrate Specificity; D013429:Sulfatases / Q000302:isolation & purification / Q000378:metabolism*
D000250:Adenosine Phosphosulfate; D010727:Phosphoric Diester Hydrolases; D013429:Sulfatases; D011755:Pyrophosphatases