Enzyme kinetics in single cells: concept and model.
We describe a technique whereby it is possible to measure enzyme activity in a singel cell. The model chosen involved the measurement of myeloperoxidase activity in a polymorphonuclear neutrophil leukocyte. Details of the apparatus are described. The experiments necessary to optimize the reaction conditions are summarized. The nature of the phases of the reaction are described. The technique appears to have further application in measurement of the activities of other enzymes in leukocytes or other cells.
D003720:Densitometry; D006651:Histocytochemistry / Q000295:instrumentation / Q000379:methods; D006801:Humans; D007700:Kinetics; D009504:Neutrophils / Q000201:enzymology*; D009195:Peroxidase / Q000097:blood*; D010544:Peroxidases / Q000097:blood*
D010544:Peroxidases; D009195:Peroxidase