Effect of cyclic AMP on placental active transport of amino acid in rats.
In pursuit of the probable relationship of cAMP to the placental active transport of amino acids as suggested by our previous human data, experiments were done in this study with dibutyryl cAMP and theophylline loaded in pregnant rats to examine the changes in level of 14C-lysine in the maternal liver, placenta and fetus. The results are: (1) Loading of dibutyryl cAMP had no effect on the uptake of 14C-lysine in the maternal liver, placenta or fetus, except that the correction of the values with its concentration in the maternal blood revealed only a slightly significant elevation in the value in each of them 30 min after the loading. (2) Loading of theophylline significantly increased the uptake of 14C-lysine in all these organs. (3) The content of cAMP in the placenta significantly increased with the loading of theophylline. These results have led the authors to believe that endogenous cAMP is significantly linked with the placental active transport of amino acids, though there is no apparent contribution to it by exogenous cAMP.
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D001693:Biological Transport, Active; D003994:Bucladesine / Q000494:pharmacology; D000242:Cyclic AMP / Q000502:physiology*; D005260:Female; D005333:Fetus / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D051381:Rats; D013806:Theophylline / Q000494:pharmacology
D000596:Amino Acids; D003994:Bucladesine; D013806:Theophylline; D000242:Cyclic AMP; D008239:Lysine