title: Analysis of DNA structure by hydroxyapatite columns and ethidium bromide fluorescence techniques. A comparative study and effect on DNA binding.
abstract: Seven duplex DNA preparations have been structurally analyzed by hydroxyapatite column chromatography and an ethidium bromide fluorescence technique. Significant contamination of one preparation with single-stranded DNA was detected by hydroxyapatite column chromatography. Five of the other six preparations were found to contain significant single-stranded regions by the ethidium bromide fluorescence technique. Synthetic poly dAT was found to be duplex in structure. The presence of single-stranded regions considerably influenced DNA binding results in a radioimmunoassay.
mesh_terms: D000906:Antibodies; D000918:Antibody Specificity; D002845:Chromatography / Q000379:methods*; D004247:DNA / Q000032:analysis* / Q000276:immunology; D004265:DNA Helicases; D004261:DNA Replication; D004277:DNA, Single-Stranded / Q000032:analysis; D004996:Ethidium; D006801:Humans; D006882:Hydroxyapatites; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D008968:Molecular Conformation; D011071:Poly T / Q000032:analysis; D011485:Protein Binding; D011863:Radioimmunoassay / Q000379:methods*
chemical_list: D000906:Antibodies; D004277:DNA, Single-Stranded; D006882:Hydroxyapatites; D011071:Poly T; D004247:DNA; D004265:DNA Helicases; D004996:Ethidium