Extraction and analytic procedures for cytosine arabinoside and 1-beta-D-arabinofuranosyluracil and their 5'-mono-, di-, and tri-phosphates.
A qualitative and quantitative comparison of perchloric acid (PCA) and trichloroacetic acid (TCA) extraction procedures of biologic material containing cytosine arabinoside (Ara-C) and 1-beta-D-arabinofuranosyluracil (Ara-U) and their biologic 5'-phosphate derivatives revealed definite superiority of the PCA procedure over the TCA procedure. High-pressure liquid chromatography provides rapid and accurate quantitative and qualitative separation of both Ara-C and Ara-U and their 5'-mono-, di-, and tri-phosphates (mu Bondapak NH2 column), or their 5'-mono-, di-, and tri-phosphates without nucleosides (ABX Permaphase columns).
D000818:Animals; D001086:Arabinofuranosyluracil / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D002854:Chromatography, Paper; D003561:Cytarabine / Q000032:analysis*; D003597:Cytosine Nucleotides / Q000032:analysis*; D008722:Methods; D051379:Mice; D010472:Perchlorates; D011741:Pyrimidine Nucleosides / Q000032:analysis*; D014238:Trichloroacetic Acid; D014500:Uracil Nucleotides / Q000032:analysis*
D003597:Cytosine Nucleotides; D010472:Perchlorates; D011741:Pyrimidine Nucleosides; D014500:Uracil Nucleotides; D003561:Cytarabine; D001086:Arabinofuranosyluracil; D014238:Trichloroacetic Acid