Regulation of acetylcholine release from neuroblastoma x glioma hybrid cells.
Neuroblastoma x glioma NG108-15 hybrid cells exposed to N6, O2'-dibutyryladenosine 3':5'-cyclic monophosphate for several days release [3H]acetylcholine in response to serotonin, prostaglandin F2alpha, KCl, or veratridine. NG108-15 cells grown in the absence of dibutyrul cyclic AMP do not respond to an excitatory stimulus by releasing [3H]acetylcholine but can be shifted to a responsive state by treatment with dibutyryl cyclic AMP. Thus, the reactions that are required for acetylcholine release can be regulated in NG108-15 cells, thereby regulating the ability of cells to form synapses and the efficiency of synaptic communication.
D000109:Acetylcholine / Q000378:metabolism*; D001692:Biological Transport / Q000187:drug effects; D003994:Bucladesine / Q000494:pharmacology*; D002118:Calcium / Q000494:pharmacology; D002460:Cell Line; D002794:Choline / Q000378:metabolism; D006822:Hybrid Cells; D009474:Neurons / Q000378:metabolism*; D011188:Potassium / Q000494:pharmacology; D011453:Prostaglandins / Q000494:pharmacology; D012701:Serotonin / Q000494:pharmacology; D013570:Synaptic Membranes / Q000378:metabolism; D013779:Tetrodotoxin / Q000494:pharmacology; D014701:Veratridine / Q000494:pharmacology
D011453:Prostaglandins; D012701:Serotonin; D013779:Tetrodotoxin; D003994:Bucladesine; D014701:Veratridine; D002794:Choline; D000109:Acetylcholine; D011188:Potassium; D002118:Calcium