title: Lactoperoxidase-catalyzed iodination of horse cytochrome c:monoiodotyrosyl 74 cytochrome c.
abstract: Iodination of horse cytochrome c with the lactoperoxidase-hydrogen peroxide-iodide system results initially in the formation of the monoiodotyrosyl 74 derivative. This singly modified protein was obtained in pure form by ion exchange chromatography and preparative column electrophoresis. It shows an intact 695 nm absorption band, the midpoint potential of the native protein, a nuclear magnetic resonance spectrum which indicates an undisturbed heme crevice structure, a normal reaction with antibodies directed against native horse cytochrome c, and circular dichroic spectra in which the only changes from those of the native protein can be ascribed to the spectral properties of iodotyrosine itself. This conformationally intact derivative reacts with the succinate-cytochrome c reductase and the cytochrome c oxidase systems of beef mitochondrial particle preparations indistinguishably from the unmodified protein, showing that the region including tyrosine 74 is not involved in these enzymic electron transfer functions of the protein. The circular dichroic spectra of this derivative indicate that the minima observed at 288 and 282 nm in the spectrum of native ferricytochrome c originate from tyrosyl residue 74.
mesh_terms: D000818:Animals; D002942:Circular Dichroism; D003574:Cytochrome c Group / Q000378:metabolism; D004579:Electron Transport; D006736:Horses; D007784:Lactoperoxidase; D007470:Monoiodotyrosine; D009206:Myocardium / Q000201:enzymology; D010446:Peptide Fragments / Q000032:analysis; D010544:Peroxidases; D011487:Protein Conformation; D013056:Spectrophotometry, Ultraviolet; D014443:Tyrosine
chemical_list: D003574:Cytochrome c Group; D010446:Peptide Fragments; D014443:Tyrosine; D007784:Lactoperoxidase; D010544:Peroxidases; D007470:Monoiodotyrosine