title: Subclones of normal clonal prolactin cells of the rat.
abstract: We recently established a clone (2B8) of normal rat prolactin cells that secretes only prolactin into the medium. When grown in the presence of thyrotrophin-releasing hormone (TRH), estradiol (E2) or arginine vasotocin (AVT), the cells show increased production of prolactin. Subclones of single cell origin were developed from 2B8 cells exposed for 1 week to TRH, E2 or TRH plus E2. These subclones differ in their response to TRH, E2 or AVT and therefore may possess different receptors for these hormones.
mesh_terms: D002999:Clone Cells / Q000187:drug effects / Q000378:metabolism*; D004958:Estradiol / Q000494:pharmacology; D010902:Pituitary Gland / Q000166:cytology*; D011388:Prolactin / Q000378:metabolism*; D011956:Receptors, Cell Surface; D011960:Receptors, Estrogen; D013973:Thyrotropin-Releasing Hormone / Q000494:pharmacology; D014668:Vasotocin / Q000494:pharmacology
chemical_list: D011956:Receptors, Cell Surface; D011960:Receptors, Estrogen; D004958:Estradiol; D013973:Thyrotropin-Releasing Hormone; D011388:Prolactin; D014668:Vasotocin