Acid glycosidase and arylsulfatase activities of human cerebrospinal fluid as measured by concanavalin A-sepharose affinity chromatography.
An affinity chromatographic method using concanavalin A-Sepharose is described for the determination of N-acetyl-beta-D-glucosaminidase, arylsulfatase. alpha-L-Fucosidase and alpha-D-mannosidase activities in the human cerebrospinal fluid. By this method (starting with 12 to 20 ml samples of cerebrospinal fluid) the above enzymes could be obtained in a concentrated form and their activities could be determined within incubation periods of 30 min to 1 h under the assay conditions described. The pH optima of the enzymes were in the range of pH 4 to 5. About 80% of the total cerebrospinal fluid N-acetyl-beta-D-glucosaminidase was found to be the A form by DEAE-Sephadex A-50 chromatography. About 60% of the total arylsulfatase was also found to be the A form. Determination of these enzyme activities in a few samples of human cerebrospinal fluid indicated a rough proportionality between the enzyme activities and the protein concentration in the cerebrospinal fluid.
D000118:Acetylglucosaminidase / Q000134:cerebrospinal fluid; D000328:Adult; D001192:Arylsulfatases / Q000134:cerebrospinal fluid*; D002846:Chromatography, Affinity; D002852:Chromatography, Ion Exchange; D003208:Concanavalin A; D006026:Glycoside Hydrolases / Q000134:cerebrospinal fluid*; D006801:Humans; D008361:Mannosidases / Q000134:cerebrospinal fluid; D012685:Sepharose; D013429:Sulfatases / Q000134:cerebrospinal fluid*; D000519:alpha-Galactosidase / Q000134:cerebrospinal fluid; D005644:alpha-L-Fucosidase / Q000134:cerebrospinal fluid; D001616:beta-Galactosidase / Q000134:cerebrospinal fluid; D001617:beta-Glucosidase / Q000134:cerebrospinal fluid
D003208:Concanavalin A; D012685:Sepharose; D013429:Sulfatases; D001192:Arylsulfatases; D006026:Glycoside Hydrolases; D008361:Mannosidases; D001617:beta-Glucosidase; D000519:alpha-Galactosidase; D001616:beta-Galactosidase; D005644:alpha-L-Fucosidase; D000118:Acetylglucosaminidase