title: A large product of cell-free translation of messenger RNA coding for corticotropin.
abstract: Polyadenylate-containing RNA prepared from the membrane fraction of bovine anterior pituitary gland was shown to direct the synthesis of a large translation product related to corticotropin (adrenocorticotropic hormone) in a cell-free system derived from wheat germ. This product was identified by immunoprecipitation with specific antibody against corticotropin, followed by electrophoretic analysis of the dissociated immunoprecipitate on sodium dodecyl sulfate-polyacrylamide gel. Further evidence for the identity of the translation product was provided by the presence of a common peptide in the chymotryptic digest of [35S]methionine-labeled cell-free product and in that of authentic corticotropin. The molecular weight of the translation product was estimated to be approximately 35,000, based on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. These results indicate that corticotropin messenger RNA directs the cell-free synthesis of a product that contains the amino acid sequence of corticotropin but is much larger than this hormone.
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000096:biosynthesis* / Q000276:immunology; D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D000918:Antibody Specificity; D002417:Cattle; D002474:Cell-Free System; D004721:Endoplasmic Reticulum / Q000378:metabolism; D008970:Molecular Weight; D010903:Pituitary Gland, Anterior / Q000032:analysis / Q000648:ultrastructure; D011132:Polyribosomes / Q000378:metabolism; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000378:metabolism*
chemical_list: D000906:Antibodies; D011498:Protein Precursors; D012333:RNA, Messenger; D000324:Adrenocorticotropic Hormone