Solid-phase enzyme-linked immunosorbent assay for detection of hepatitis A virus.
An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of hepatitis A virus in human fecal specimens. Investigations with 88 fecal specimens from 77 patients with suspected viral hepatitis and 8 of their household contacts showed that ELISA was as specific and sensitive as radioimmunoassay and almost as sensitive as immune electron microscopy. The ELISA is quick and simple to perform, does not require sophisticated technical equipment, and can be read with the naked eye, making it suitable for field work and rapid diagnosis.
D000937:Antigen-Antibody Reactions; D003937:Diagnosis, Differential; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000276:immunology; D006525:Hepatitis, Viral, Human; D006507:Hepatovirus / Q000302:isolation & purification*; D006801:Humans; D007124:Immunoenzyme Techniques; D008854:Microscopy, Electron; D011863:Radioimmunoassay