Hydrosmotic effect of angiotensin II in the toad skin: role of cyclic AMP.
The mechanism of action of the hydrosmotic response of the isolated skin of the toad Bufo arenarum Hensel to angiotensin II was studied by means of an indirect pharmacological approach. Angiotensin II (2.10(-10) M), vasopressin (2.10(-13) M) and theophylline (10(-4) and 10(-3) M) in subliminal doses produced a significant increase on water permeability when added in different paired combinations. Angiotensin II (2.10(-7) M) and vasopressin (2.10(-8) M) in doses producing significant effects on water permeability increased the response to submaximal doses of epinephrine (10(-6) M) but not to higher doses (10(-5) M). Acid pH (6.4) and prostaglandin E1 (2.10(-7) M) reduced significantly the hydrosmotic response to angiotensin II, but in contrast with the toad bladder, the effect was not completely abolished. Present results support the view that the hydrosmotic effect of angiotensin II in toad skin is mediated by the adenylate cyclase - cyclic AMP system.
D000262:Adenylyl Cyclases / Q000494:pharmacology; D000804:Angiotensin II / Q000494:pharmacology*; D000818:Animals; D002022:Bufo arenarum; D002463:Cell Membrane Permeability / Q000187:drug effects*; D000242:Cyclic AMP / Q000494:pharmacology*; D004357:Drug Synergism; D004847:Epithelial Cells; D004848:Epithelium / Q000187:drug effects; D012867:Skin / Q000187:drug effects* / Q000201:enzymology; D013806:Theophylline / Q000494:pharmacology; D013997:Time Factors; D014667:Vasopressins / Q000494:pharmacology
D014667:Vasopressins; D000804:Angiotensin II; D013806:Theophylline; D000242:Cyclic AMP; D000262:Adenylyl Cyclases