In vitro translation yields a possible Rous sarcoma virus src gene product.
In vitro translation of Rous sarcoma virus (RSV) virion RNA in the messenger-dependent reticulocyte lysate system yielded polypeptides that were not synthesized by translation of RNA from a transformation-defective deletion mutant of RSV. These RSV-specific products migrated on sodium dodecyl sulfate/polyacrylamide gels as two doublets of approximately 25,000 and 17,000 daltons. Synthesis of these proteins was not sensitive to inhibition by m7GTP; however, synthesis of the 76,000-dalton precursor of the internal structural proteins was sensitive to inhibition by m7GTP. Tryptic peptide maps showed the 25,000- and 17,000-dalton proteins to be related to one another but to be distinct from the 76,000-dalton protein. The 25,000-dalton protein was translated only from a polyadenylylated RNA of approximately 2500 nucleotides, whereas the 76,000-dalton protein was translated from 38S RNA, corresponding to the entire viral genome. A 180,000-dalton protein was also synthesized from 38S RSV virion RNA. From the absence of the 25,000- and 17,000-dalton proteins in the translation products of transformation-defective RSV RNA and the size of their RNA templates, we conclude that these proteins may be derived from coding sequences within the RSV src gene.
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000378:metabolism*; D005814:Genes, Viral; D006160:Guanosine Triphosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D008970:Molecular Weight; D010452:Peptide Biosynthesis; D010446:Peptide Fragments / Q000032:analysis; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000378:metabolism*; D012156:Reticulocytes / Q000378:metabolism; D014357:Trypsin
D010446:Peptide Fragments; D012367:RNA, Viral; D006160:Guanosine Triphosphate; D014357:Trypsin