The role of adherent cells in the secondary cell-mediated response in vitro to a natural poxvirus pathogen.
The role of adherent cells in an in vitro secondary response to ectromelia virus infection was investigated. Spleen cells from ectromelia-primed mice ("responder" cells) depleted of adherent cells by either carbonyl iron treatment, adherence to plastic or passage through cotton wool columns had a markedly decreased capacity to produce a secondary response, as indicated by decreased T cell-mediated cytotoxicity against virus-infected target cells, when cultured with virus-infected "stimulator" cells. The secondary response was restored by the addition of peritoneal cells from either normal or ectromelia-immune mice. Small numbers of peritoneal cells completely reconstituted the response within a certain dose range but larger numbers produced a marked inhibition of the response. Spleen cells were less effective in restoring the response. The peritoneal cells were not merely acting as additional, infected "stimulator" or antigen-presenting cells, since they could be added as late as 3 days after culture. Reconstituting activity was not affected by pretreatment with anti-theta serum and complement and cell separation studies showed that the activity was associated mainly with Ig-negative cells and that the active cell probably bears Ia antigens on its surface. These results indicate that the adherent cells involved are probably macrophages and that they act non-specifically to produce optimum conditions for the specific response of T cells.
D000818:Animals; D000956:Antigens, Viral; D001202:Ascitic Fluid / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D002448:Cell Adhesion; D004481:Ectromelia virus / Q000276:immunology; D004482:Ectromelia, Infectious / Q000276:immunology*; D007111:Immunity, Cellular; D007156:Immunologic Memory; D066298:In Vitro Techniques; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D011213:Poxviridae Infections / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D000956:Antigens, Viral