title: [Fluorimetric determination of the concentration of histamine and serotonin in the mast cells of ordinary and germ-free rats following administration of reserpine and cyclic adenosine-3',5'-monophosphate].
abstract: The microspectrometric method was applied to the determination of the content of histamine (a test with orthophthalic aldehyde) and serotonin (Falk's test) in the mast cells of the mesentery of common and germfree rats. Reserpine (10 mg/kg of body weight, intramuscularly) failed to alter histamine content, whereas serotonin content fell by 50%. Administration of cyclic AMP (3.5 mg/kg of body weight, intraperitoneally) led to the increase in serotonin content by 80%, and histamine--by 280--320%.
mesh_terms: D000818:Animals; D000242:Cyclic AMP / Q000494:pharmacology*; D005856:Germ-Free Life; D006632:Histamine / Q000378:metabolism*; D008407:Mast Cells / Q000378:metabolism*; D051381:Rats; D012110:Reserpine / Q000494:pharmacology*; D012701:Serotonin / Q000378:metabolism*; D013050:Spectrometry, Fluorescence
chemical_list: D012701:Serotonin; D006632:Histamine; D012110:Reserpine; D000242:Cyclic AMP
vernacular_title: Fliuorimetricheskoe opredelenie soderzhaniia gistamina i serotonia v tuchnykh kletkakh obychnykh i bezmikrobnykh krys pri vozdeĭstvii rezerpinom i tsiklicheskim adenozin-3',5'-monofosfatom