Effect of cyclic AMP on glycogen phosphorylase in Coprinus macrorhizus.
Glycogen phosphorylase (1,4-alpha-D-glucan:orthophosphate alpha-glucosyltransfase, EC 2.4.1.1) activity was found in mycelial extracts of Coprinus macrorhizus concurrently with decrease of glycogen content in mycelial cells. Incubation of the enzyme sample with cyclic AMP and ATP leads to a 3-fold activation of the glucogen phosphorylase activity. Activation of the enzyme partially purified through Sepharose 6B required a cellular fraction containing cyclic AMP-dependent protein kinase.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000363:Agaricales / Q000201:enzymology*; D002455:Cell Division; D003302:Coprinus / Q000201:enzymology*; D000242:Cyclic AMP / Q000494:pharmacology*; D003624:Darkness; D007700:Kinetics; D008027:Light; D006005:Phosphorylases / Q000302:isolation & purification / Q000378:metabolism*; D013045:Species Specificity; D013997:Time Factors
D000255:Adenosine Triphosphate; D000242:Cyclic AMP; D006005:Phosphorylases