Induction of latent Herpes simplex virus type 2 infection in human cervical epithelial cells in vitro.
An in vitro method was used to induce non-productive herpes simplex virus type 2 (HSV-2) infections in human ecto- and endocervical epithelial cells. Adenine arabinoside (ara-A) could prevent the replication of HSV-2 in ecto- and endocervical cells and after removal of ara-A from growth medium a rapid destruction of explants with development of infectious virus was observed. When ara-A was not withdrawn until 4 days or later after in vitro infection the morphological, immunofluorescent and virological studies detected no infections. However, 2-3 weeks after inoculation there were in some cultures morphological as well as immunofluorescent signs of HSV infection without demonstrable infectious virus, indicating an incidental abortive nature of the infective process.
D002478:Cells, Cultured; D002584:Cervix Uteri / Q000166:cytology / Q000382:microbiology*; D004848:Epithelium / Q000382:microbiology; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D066298:In Vitro Techniques; D018139:Simplexvirus; D014740:Vidarabine / Q000494:pharmacology; D014776:Virus Cultivation; D014779:Virus Replication / Q000187:drug effects
D014740:Vidarabine