[Mechanism of action of ouabain and cyclic-3',5'-adenosine monophosphate on calcium ion transport in rat cardiac mitochondria].
Ouabain and cyclic AMP action on the Ca2+-accumulating capacity and the efflux of Ca2+ions from the loaded mitochondria of the rat heart was investigated by the tetracycline probe method. On the basis of the experimental results a conclusion was drawn that ouabain failed to act on these processes. Cyclic AMP didn't act on the Ca binding by the mitochondrial membrane; at the same time it induced rapid release of calcium from the loaded mitochondria.
D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002118:Calcium / Q000378:metabolism*; D000242:Cyclic AMP / Q000494:pharmacology*; D008928:Mitochondria / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism / Q000648:ultrastructure*; D010042:Ouabain / Q000494:pharmacology*; D051381:Rats
D010042:Ouabain; D000242:Cyclic AMP; D002118:Calcium
Issledovanie mekhanizma deĭstviia uabaina i tsiklicheskogo adenozin-3',5'-monofosfata na transport ionov kal'tsiia mitokhondriiami serdtsa krys