Nephritogenic glycoprotein. V. Immunochemical studies on nephritogenic activity of collagenase and pronase-digests prepared from various rat organs.
A new immunochemical procedure was introduced to estimate the nephritogenic activity of collagenase and pronase digests of various rat organs. A glycoprotein isolated from collagenase digests of various rat organs showed the nephritogenic activity as well as the antigenic activity that induces nephrotoxic antibody, which were nearly identical to those of a glycoprotein isolated from trypsin digests of the rat organ concerned. A glycoprotein isolated from pronase digests of various rat organs was proved to have no antigenic activity that induces nephrotoxic antibody, but the existence of nephritogenic activity was proved in this glycoprotein. These results were supported firmly by the assay experiments for nephritogenicity. Ouchterlony gel diffusion test and the assay experiments demonstrated the existence of a common nephritogenic substance among the glycoproteins isolated from trypsin, collagenase and pronase digests of rat organs.
D000818:Animals; D000906:Antibodies; D000917:Antibody Formation; D000918:Antibody Specificity; D004586:Electrophoresis; D005921:Glomerulonephritis / Q000276:immunology; D006023:Glycoproteins / Q000032:analysis* / Q000276:immunology; D007124:Immunoenzyme Techniques; D007668:Kidney / Q000276:immunology; D008297:Male; D003012:Microbial Collagenase / Q000378:metabolism; D011402:Pronase / Q000378:metabolism; D051381:Rats
D000906:Antibodies; D006023:Glycoproteins; D011402:Pronase; D003012:Microbial Collagenase