Solid-phase enzyme immunoassay for immunoglobulin M antibodies to cytomegalovirus.
A sensitive, solid-phase enzyme immunoassay for the detection of immunoglobulin M antibodies to cytomegalovirus is described. The results of the enzyme immunoassay correlated well with those obtained by an indirect immunofluorescence method. Horseradish peroxidase proved to be a more sensitive label than alkaline phosphatase. Nonspecific reactions, occurring with commercially available cytomegalovirus antigens, could be avoided by using a nuclear antigen prepared from sonically disrupted nuclei of cytomegalovirus-infected cells.
D000469:Alkaline Phosphatase; D000914:Antibodies, Viral / Q000032:analysis*; D000918:Antibody Specificity; D003587:Cytomegalovirus / Q000276:immunology*; D005069:Evaluation Studies as Topic; D005455:Fluorescent Antibody Technique; D006735:Horseradish Peroxidase; D006801:Humans; D007124:Immunoenzyme Techniques; D007075:Immunoglobulin M / Q000032:analysis*
D000914:Antibodies, Viral; D007075:Immunoglobulin M; D006735:Horseradish Peroxidase; D000469:Alkaline Phosphatase