title: Isolation of living adult Onchocerca volvulus from nodules.
abstract: A technique for the isolation of adult Onchocerca volvulus from excised onchocercomata is described. The nodules are incubated in medium 199 containing 1-5 mg collagenase and 0.2 mg gentamicin per ml for 6-48 hours in a waterbath at 30-37 degrees C. A proportion of the worms can be isolated alive.
mesh_terms: D000818:Animals; D003470:Culture Media; D005260:Female; D005839:Gentamicins; D006801:Humans; D003012:Microbial Collagenase; D009854:Onchocerca / Q000302:isolation & purification* / Q000502:physiology; D051381:Rats; D013696:Temperature
chemical_list: D003470:Culture Media; D005839:Gentamicins; D003012:Microbial Collagenase