[Synthesis of RNA fractions in rat liver during single and multiple stimulation of the hypothalamus].
Specific radioactivity of RNA from rat liver tissue, labelled with 14C-orotic acid and fractionated using thermic phenol method, was altered dissimilarly. In single stimulation of hypothalamus the specific radioactivity of nuclear RNA was increased in RNA-40 degrees fraction than in RNA-55 degrees and RNA-63 degrees ones. A decrease in synthesis of nuclear RNA fractions followed the phase of activation in repeated stimulation of hypothalamus. The distinct increase in specific radioactivity was observed in cytoplasmic RNA (RNA-4 degrees) both in single and repeated stimulation of hypothalamus. The stimulating effect of the prolonged hypothalamus irritation on synthesis of the RNA fractions and on activity of glucose-6-phosphatase and fructose-1,6-diphosphatase disappeared more rapidly than this effect in long-term administration of hydrocortisone. The data obtained suggest that sensitivity of tissue-targets to prolonged hormonal stimulation is distinctly higher than the ability of hypothalamus to activate the synthesis of RNA and enzymatic proteins.
D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000818:Animals; D004558:Electric Stimulation; D005636:Fructosephosphates / Q000378:metabolism; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D006854:Hydrocortisone / Q000494:pharmacology; D007031:Hypothalamus / Q000502:physiology*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D012313:RNA / Q000096:biosynthesis*; D051381:Rats
D005636:Fructosephosphates; D012313:RNA; D000324:Adrenocorticotropic Hormone; D005952:Glucose-6-Phosphatase; D006854:Hydrocortisone
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