Study of group-specific proteins in mammalian cells transformed by Rous sarcoma virus.
Lysates of various mammalian RSV-transformed cells labelled with 35S-methionine were tested for the presence of gs proteins and for the size of these molecules by means of antisera, using sodium dodecyl sulphate--polyacrylamide slab gel electrophoresis. In no case was the presence of gs proteins detected in the analyzed immunoprecipitates; only in RSH-mix cells a quantitative difference in the contrast of a 70 000-molecular weight protein was found. The autoradiograms of simultaneously tested immunoprecipitates of lysates of RSV-transformed chicken cells clearly showed the presence of p27 and after a short-term labelling also of the gs precursor molecule (pr76). The cause of the failure in detecting gs proteins by the used method in two mammalian RSV-transformed lines, in which the gs antigens were previously detected by complement fixation test, are discussed.
D001358:Avian Sarcoma Viruses / Q000378:metabolism; D002460:Cell Line; D002472:Cell Transformation, Viral; D007158:Immunologic Techniques; D008970:Molecular Weight; D011498:Protein Precursors / Q000378:metabolism; D014764:Viral Proteins / Q000276:immunology / Q000378:metabolism*
D011498:Protein Precursors; D014764:Viral Proteins