[Purification of calf thymus adenosine diphosphate ribose polymerase].
Calf thymus poly ADPR polymerase has been purified to electrophoretic homogenity. The enzyme has a molecular weight of 120,000 +/- 10,000 dalton. The substrate affinity is very high (apparent Km 82.5 micrometer). The presence of exogenous DNA does not appear to be a requisite for enzymatic activity of the purified enzyme.
D000818:Animals; D002417:Cattle; D007700:Kinetics; D008970:Molecular Weight; D009244:NAD+ Nucleosidase / Q000302:isolation & purification*; D011065:Poly(ADP-ribose) Polymerases / Q000302:isolation & purification*; D013950:Thymus Gland / Q000201:enzymology*
D011065:Poly(ADP-ribose) Polymerases; D009244:NAD+ Nucleosidase
Purification de l'adénosine diphosphate ribose polymérase de thymus de Veau.