Studies on reduction of lipolysis in adipose tissue on freezing and thawing.
Adrenaline-induced lipolysis in fat cells was remarkably reduced when the cells were preincubated in a dry ice-aceton bath, but their adenylcyclase and lipase activities were not reduced. In the reconstructed lipid micelles which consisted of lipase-depleted lipid micelles and lipase-containing adipose tissue extract, adrenaline, theophylline and DBcAMP-induced lipolysis was not found when lipase-depleted lipid micelles were preincubated in a dry ice-aceton bath but was found when lipase was preincubated.
D000262:Adenylyl Cyclases / Q000378:metabolism; D000273:Adipose Tissue / Q000201:enzymology / Q000378:metabolism*; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D004837:Epinephrine / Q000494:pharmacology; D005615:Freezing; D066298:In Vitro Techniques; D008049:Lipase / Q000378:metabolism; D050356:Lipid Metabolism; D008297:Male; D051381:Rats; D013806:Theophylline / Q000494:pharmacology
D003994:Bucladesine; D013806:Theophylline; D008049:Lipase; D000262:Adenylyl Cyclases; D004837:Epinephrine