Synthesis of Epstein-Barr virus DNA in vitro: effects of phosphonoacetic acid, N-ethylmaleimide, and ATP.
Nuclei from superinfected Raji cells synthesized Epstein-Barr Virus (EBV) DNA in vitro in the absence of cell DNA synthesis. The synthesis of EBV DNA in vitro was inhibited by phosphonoacetic acid and N-ethylmaleimide, and maximum synthesis was achieved in the absence of an ATP-regenerating system. Nuclei from mock-infected cells required an ATP-regenerating system for maximum DNA synthesis.
D000085:Acetates / Q000494:pharmacology*; D000255:Adenosine Triphosphate / Q000378:metabolism*; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D002474:Cell-Free System; D004261:DNA Replication / Q000187:drug effects; D004279:DNA, Viral / Q000096:biosynthesis*; D005033:Ethylmaleimide / Q000494:pharmacology*; D004854:Herpesvirus 4, Human / Q000378:metabolism*; D009943:Organophosphorus Compounds / Q000494:pharmacology*
D000085:Acetates; D004279:DNA, Viral; D009943:Organophosphorus Compounds; D000255:Adenosine Triphosphate; D005033:Ethylmaleimide