Simultaneous serum and CSF antibodies in herpes simplex virus encephalitis.
Serial concomitant paired sera (S) and CSF were taken from eight patients with biopsy-proved herpes simplex virus encephalitis (HSVE). These specimen pairs were compared with 28 others from patients with various neurologic conditions. Before and after reduction with 2-mercaptoethanol, a ratio of S/CSF antibody titers of less than or equal to 20 with either the passive hemagglutinating (PHA) or immune adherence hemagglutinating (IAHA) antibody tests occurred in every patient with HSVE. Diagnostic S/CSF ratios were noted in three patients before biopsy of the brain and in four patients by the tenth day of neurologic disease. Among control subjects, a ratio of S/CSF titers greater than 20 was observed in all but four patients. Each of the latter patients had neurologic diagnoses easily distinguishable from HSVE. The PHA or IAHA S/CSF ratio offers a rapid, reliable method for the diagnosis of HSVE (P less than .001).
D000328:Adult; D000914:Antibodies, Viral / Q000032:analysis* / Q000134:cerebrospinal fluid*; D001706:Biopsy; D001921:Brain / Q000473:pathology; D004660:Encephalitis / Q000175:diagnosis* / Q000209:etiology / Q000276:immunology; D005260:Female; D006386:Hemagglutination Tests; D006561:Herpes Simplex / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D006801:Humans; D007104:Immune Adherence Reaction; D008297:Male; D018139:Simplexvirus / Q000276:immunology* / Q000302:isolation & purification
D000914:Antibodies, Viral