Strandedness of newly synthesized short pieces of polyoma DNA from isolated nuclei.
The discontinuous synthesis of the complementary strands of polyoma DNA in isolated nuclei has been studied by hybridization techniques. The relative amounts of the newly synthesized complementary strands were compared by separately annealing them to denatured HpaII restriction fragments. In every case an excess (1.4- to 2.4-fold) of short pieces of the strand growing in the 3' leads to 5' direction was found.
D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D004270:DNA, Circular / Q000096:biosynthesis / Q000302:isolation & purification; D004277:DNA, Single-Stranded / Q000096:biosynthesis / Q000302:isolation & purification; D004279:DNA, Viral / Q000096:biosynthesis* / Q000302:isolation & purification; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D009695:Nucleic Acid Renaturation; D011120:Polyomavirus / Q000378:metabolism*
D004270:DNA, Circular; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D004262:DNA Restriction Enzymes