Transformation of Chinese hamster embryo cells with an avian sarcoma virus ts mutant.
Chinese hamster embryo cells were transformed with temperature-sensitive mutant of the Schmidt-Ruppin strain of Rous sarcoma virus-subgroup A (ts NY68S Ra-A). The developed cell line elicited the morphological transformation at a permissive temperature of 35 degrees C, whereas after a shift to a non-permissive temperature of 40 degrees C the cells reverted to a normal phenotype. Tests for the presence of avian sarcoma group-specific antigen showed that the antigen was not expressed both at permissive and non-permissive temperature. However, the transient expression of gs antigen could be achieved by treatment with 5-IUdR only at the permissive temperature. There was a difference in the rate of 14C-2-deoxy-D-glucose uptake between cells cultured at the permissive and non-permissive temperature.
D000818:Animals; D000956:Antigens, Viral; D001358:Avian Sarcoma Viruses; D002460:Cell Line; D002642:Chick Embryo; D006224:Cricetinae; D003588:Cytopathogenic Effect, Viral; D004622:Embryo, Mammalian; D005947:Glucose / Q000378:metabolism; D013696:Temperature
D000956:Antigens, Viral; D005947:Glucose