Modification of the Warburg's flask for the study of electric current effect upon the activity of enzymatic systems.
Modifications of the Warburg's flask were suggested for the study of the electric current effect upon enzymatic systems under directly stimulation "in vitro" and "in vivo" conditions. The proposed modifications made on the Warburg's flask were basically constituted by the insertion of two platinum and mercury electrodes in the principal reservoir. In order to demonstrate the efficiency and viability of the suggested modification, one experience concerning the effect of the electric current upon the cerebral glutamate decarboxylase activity was shown. The apoenzyme substrate system was activated 1.81 times by the electrical stimulation in absence of the coenzyme. In a similar way the complete system, that is, the apoenzyme-coenzyme-substrate was also stimulated. The physical phenomena seem to be of importance for the understanding of the physiology and biochemistry of the nervous tissue.
D000818:Animals; D001921:Brain / Q000201:enzymology*; D004558:Electric Stimulation / Q000295:instrumentation* / Q000379:methods; D003576:Electron Transport Complex IV / Q000378:metabolism*; D051379:Mice; D010101:Oxygen Consumption
D003576:Electron Transport Complex IV