title: Susceptibility of chickens to avian encephalomyelitis virus. IV. Behavior of the virus in laying hens.
abstract: Some laying hens 6 months of age were inoculated subcutaneously or orally with a chick embryo--adapted strain of avian encephalomyelitis virus and examined for propagation of the virus in the body. When inoculated subcutaneously, the virus appeared in liver, spleen, ovarian follicle, and muscle at the site of inoculation 1 day, in kidney and lumbar part of the spinal cord 3 days, in the pancreas 5 days, in heart, duodenum, and cervical part of the spinal cord 7 days, and in the brain 11 days after inoculation. After its appearance, it increased gradually in amount in liver, spleen, pancreas, muscle at the site of inoculation, and cervical and lumbar parts of the spinal cord, but remained at a low level in any other organ. When examined 14 days after inoculation and later, it was distributed mainly in the central nervous system. It was detected from 12 of 16 organs examined. The highest virus level in each organ was 10(2.6)/0.1 g in pancreas and lumbar part of the spinal cord, which were followed by muscle at the site of inoculation (10(2.0)/0.1 g), spleen (10(1.8)/0.1 g), cervical part of the spinal cord, heart, and liver in the order listed. When inoculated orally, the virus was found sporadically in spleen, pancreas, kidney, cecum, ovarian follicle, and lumbar part of the spinal cord. The virus level was low in these organs, of which pancreas, kidney, and lumbar part of the spinal cord showed the highest virus level, or 10(1.3)/0.1 g.
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002645:Chickens; D001350:Encephalomyelitis Virus, Avian / Q000302:isolation & purification*; D004770:Enterovirus / Q000302:isolation & purification*; D004769:Enterovirus Infections / Q000382:microbiology / Q000662:veterinary*; D005260:Female; D007279:Injections, Subcutaneous; D010179:Pancreas / Q000382:microbiology; D011201:Poultry Diseases / Q000382:microbiology*; D013116:Spinal Cord / Q000382:microbiology