title: Involvement of NAD  in induced synthesis of colicin E1.
abstract: Escherichia coli K-12 nadC13 (ColE1) was starved for nicotinic acid and cellular NAD levels decreased to less than 10% of the normal. Under these conditions, induction of colicin E1 synthesis decreased to about 1% of the normal value, while 30% of the total protein synthesis remained intact. Addition of nicotinic acid reversed both the ability of the colicin induction and cellular NAD level. Induced replication of colicin E1 DNA was greatly reduced in the NAD-deprived cells.
mesh_terms: D000255:Adenosine Triphosphate / Q000096:biosynthesis; D001426:Bacterial Proteins / Q000096:biosynthesis; D003087:Colicins / Q000096:biosynthesis*; D004261:DNA Replication / Q000187:drug effects; D004269:DNA, Bacterial / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*; D008937:Mitomycins / Q000494:pharmacology; D009243:NAD / Q000378:metabolism*; D009539:Nicotinic Acids / Q000172:deficiency / Q000494:pharmacology*; D012329:RNA, Bacterial / Q000096:biosynthesis; D013941:Thymine / Q000378:metabolism; D014529:Uridine / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D003087:Colicins; D004269:DNA, Bacterial; D008937:Mitomycins; D009539:Nicotinic Acids; D012329:RNA, Bacterial; D009243:NAD; D000255:Adenosine Triphosphate; D013941:Thymine; D014529:Uridine