Biochemical analysis of a virulent and an avirulent strain of foot-and-mouth disease virus.
A comparison has been made of some of the serological and physicochemical properties of a virulent and an avirulent strain of foot-and-mouth disease virus, serotype SAT1. The avirulent strain (SAT1-82) was derived from the virulent strain (SAT1-7) by serial passage in BHK 21 cells. The viruses were indistinguishable in cross-neutralization tests. In immunodiffusion tests a clear spur line was obtained with the SAT1-82 antiserum but not with SAT1-7 antiserum. The major polypeptides of the two viruses were identical when examined by polyacrylamide gel electrophoresis. Hybridization and thermal denaturation experiments failed to distinguish between the RNAs but two-dimensional electrophoresis of the oligonucleotides produced by ribonuclease T1 digestion revealed several differences. Possibly the most significant of these differences was the size of the polycytidylic acid [poly (C)] tract. There were about 170 nucleotides in the poly (C) tract of the SAT1-7 RNA compared with around 100 in the SAT1-82 RNA. Further evidence for this deletion was provided by the slightly different behaviour of the two RNAs when compared by sucrose gradient centrifugation and polyacrylamide gel electrophoresis.
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D000956:Antigens, Viral / Q000032:analysis; D005537:Aphthovirus / Q000032:analysis* / Q000254:growth & development / Q000276:immunology; D001483:Base Sequence; D003429:Cross Reactions; D006168:Guinea Pigs; D009841:Oligonucleotides / Q000032:analysis; D010455:Peptides / Q000032:analysis; D011066:Poly C / Q000032:analysis; D011487:Protein Conformation; D012367:RNA, Viral / Q000032:analysis; D014611:Vaccination; D014764:Viral Proteins / Q000032:analysis*; D014774:Virulence; D014779:Virus Replication
D000914:Antibodies, Viral; D000956:Antigens, Viral; D009841:Oligonucleotides; D010455:Peptides; D012367:RNA, Viral; D014764:Viral Proteins; D011066:Poly C