Kinetic studies on 15-hydroxyprostaglandin dehydrogenase from human placenta.
The enzyme system 15-hydroxyprostaglandin dehydrogenase, which catalyzes the oxidation of the 15-hydroxy group of all naturally occurring prostaglandins, has been purified 1,270-fold by isoelectric focusing. Km values have been determined for prostaglandin E2 and NAD+ and were found to be 1 and 44 muM. The overall forward reaction was V1 = 450 nmol/min. Both the product 15-ketoprostaglandin E2 and the metabolite 13,14-dihydro-15-ketoprostaglandin E2 were noncompetitive inhibitors for the enzyme.
D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D005260:Female; D006801:Humans; D006911:Hydroxyprostaglandin Dehydrogenases / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D007700:Kinetics; D009243:NAD / Q000378:metabolism; D010920:Placenta / Q000201:enzymology*; D011247:Pregnancy; D011458:Prostaglandins E / Q000378:metabolism
D011458:Prostaglandins E; D009243:NAD; D000429:Alcohol Oxidoreductases; D006911:Hydroxyprostaglandin Dehydrogenases