Solubilization of the Epstein-Barr virus-determined nuclear antigen and its characterization as a DNA-binding protein.
The Eptstein-Barr virus (EBV)-determined nuclear antigen (EBNA) was solubilized from isolate nuclei of two EBV-transformed cell lines- Raji and AW-Ramos, by high-salt treatment. Its DNA-binding properties were studied by DNA-cellulose chromatography and a 51Cr release complement fixation assay. EBNA binds to both double-stranded and single-stranded calf thymus DNA, showing a higher affinity to double-stranded DNA. There was no detectable difference in the DNA binding of EBNA prepared from Raji and AW-Ramos cells.
D000956:Antigens, Viral / Q000302:isolation & purification; D002051:Burkitt Lymphoma; D002460:Cell Line; D002467:Cell Nucleus / Q000276:immunology; D003168:Complement Fixation Tests; D004247:DNA / Q000378:metabolism; D004854:Herpesvirus 4, Human / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D011485:Protein Binding; D012995:Solubility; D013501:Surface-Active Agents; D014508:Urea; D014764:Viral Proteins / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism
D000956:Antigens, Viral; D013501:Surface-Active Agents; D014764:Viral Proteins; D014508:Urea; D004247:DNA