title: Light-activated hydrolysis of GTP and cyclic GMP in the rod outer segments.
abstract: 1. The hydrolysis of guanosine triphosphate (GTP) and the consequent formation of guanosine diphosphate (GDP) and phosphate (P1) are activated by light in a suspension of broken retinal rods: the hydrolysis rate with GTP in the micrometer concentration range is 2.5-3.5 n-mole/min per mg of rhodopsin in the preparation. 2. The ionic composition of the medium suspending the rods is not critical: the hydrolysis is present in NaCl saline solution with MG2+ as well as in Tris-HC1 buffer solution, and with the chelating agent EDTA. 3. The ionic strength is critical: the effect is reduced when the broken rods are suspended in a low salt mannitol solution, and is altogether abolished when they are separated from the mannitol solution; it reappears when the mannitol solution is added again in the presence of salts. An element essential for the effect is thus reversibly released in the mannitol solution. No hydrolytic activity on GTP, however, is found in the mannitol soluble fraction. 4. The cyclic nucleotide phosphodiesterase is eluted from the rods in the mannitol solution, and is reaggregated to the rods in the presence of salts; once recombined with the rods, it can be activated by light. 5. The activation of the phosphodiesterase by light is present in the absence of added nucleotide triphosphates.
mesh_terms: D000818:Animals; D001001:Anura; D006152:Cyclic GMP / Q000378:metabolism*; D006153:Guanosine Diphosphate / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism*; D006868:Hydrolysis; D066298:In Vitro Techniques; D008027:Light; D008297:Male; D008353:Mannitol / Q000494:pharmacology; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D010786:Photoreceptor Cells / Q000378:metabolism*; D011892:Rana catesbeiana
chemical_list: D006153:Guanosine Diphosphate; D008353:Mannitol; D006160:Guanosine Triphosphate; D010727:Phosphoric Diester Hydrolases; D006152:Cyclic GMP