Differences between resting and insulin-stimulated amino acid transport in frog skeletal muscle.
We have compared some features of the resting and the insulin-stimulated uptake of alpha-aminoisobutyrate (AIB) in frog skeletal muscle. We found a substantial difference between the two processes, namely, that resting AIB uptake is Na-independent while the insulin-stimulated fraction of the AIB uptake is Na-dependent. Since the amino acid transport systems in frog skeletal muscle are poorly characterized, we have also surveyed some of their properties. One of the most interesting findings of this survey is that both the uptake and efflux of AIB are inhibited by low concentrations of PCMBS (parachloro-mercury-benzene sulfonic acid 5 X 10(-5) M). In contrast, the carrier mediated transport of basic amino acids is neither inhibited by this mercurial agent nor accelerated by insulin. The action of PCMBS strongly suggests the presence of a critical sulfhydryl group in the amino acid carrier system utilized by AIB. This group is exposed to the outside solution since PCMBS penetrates cell membranes poorly, and in addition its inhibitory actions were reverted by agents that do not penetrate the cell membrane like albumin or glutathione.
D002731:4-Chloromercuribenzenesulfonate / Q000494:pharmacology; D000596:Amino Acids / Q000378:metabolism*; D000621:Aminoisobutyric Acids / Q000378:metabolism; D000818:Animals; D001001:Anura; D001693:Biological Transport, Active / Q000187:drug effects; D002794:Choline / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology*; D008094:Lithium / Q000494:pharmacology; D009132:Muscles / Q000187:drug effects / Q000378:metabolism*; D011188:Potassium / Q000494:pharmacology; D011894:Rana pipiens; D012964:Sodium / Q000494:pharmacology; D013439:Sulfhydryl Reagents / Q000494:pharmacology; D013696:Temperature; D014325:Tromethamine / Q000494:pharmacology
D000596:Amino Acids; D000621:Aminoisobutyric Acids; D007328:Insulin; D013439:Sulfhydryl Reagents; D014325:Tromethamine; D002731:4-Chloromercuribenzenesulfonate; D008094:Lithium; D012964:Sodium; D002794:Choline; D011188:Potassium