Intramembrane particle distribution and lectin binding of glioblastoma cells after long term subculture.
Human glioblastoma cells in long-term monolayer culture showed an even distribution of intramembrane particles (IMP) on all surfaces of the plasma membrane; junctional complexes were rarely observed and rectilinear arrays were not seen. Cells treated with Con A-ferritin and Ricin II-ferritin showed an even distribution of lectin receptors and under conditions used no capping occurred. Lectin-ferritin complexes were taken up into pinocytotic vesicles. Cleaved preparations of Ricin II-ferritin treated cells showed no change in the distribution of IMP.
D002462:Cell Membrane / Q000648:ultrastructure; D002478:Cells, Cultured; D005614:Freeze Fracturing; D005909:Glioblastoma / Q000032:analysis / Q000648:ultrastructure*; D006801:Humans; D007365:Intercellular Junctions / Q000648:ultrastructure; D037102:Lectins; D008854:Microscopy, Electron; D009374:Neoplasms, Experimental; D011952:Receptors, Concanavalin A / Q000032:analysis*; D011955:Receptors, Drug / Q000032:analysis*
D037102:Lectins; D011952:Receptors, Concanavalin A; D011955:Receptors, Drug