Substrate dependence of the mechanism of EcoRI endonuclease.
The mechanism of EcoRI endonuclease is substrate dependent. At 37 degrees dissociation of the enzyme-Form II DNA intermediates of ColE1 DNA and bacteriophage G4 RFI DNA is negligible. Therefore, both DNA strands with in the EcoRI sequence are cleaved during a single binding event. However, double strand cleavage of SV40 DNA occurs without dissociation of the enzyme in only 75% of the catalytic events. This mechanistic difference presumably reflects sequence differences about the EcoRI sites of these DNA's.
D003086:Bacteriocin Plasmids; D001483:Base Sequence; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004269:DNA, Bacterial / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology; D007700:Kinetics; D013539:Simian virus 40 / Q000378:metabolism; D013379:Substrate Specificity
D004269:DNA, Bacterial; D004279:DNA, Viral; D004262:DNA Restriction Enzymes