Complement-mediated antiserum cytotoxic reactions to human chromosome 7 coded antigen(s): immunoselection of rearranged human chromosome 7 in human-mouse somatic cell hybrids.
Immunoselection via complement-dependent lysis of human-mouse somatic cell hybrids containing chromosome 7, with antisera reactive to cell surface antigen(s) coded for by chromosome 7, has resulted in growth of somatic cell hybrids containing rearranged human chromosome 7s. Investigation of these hybrids has localized the gene(s) coding for the relevant cell surface antigen(s) to the short arm of human chromosome 7. The simian virus 40 integration site and the gene coding for human beta-glucuronidase appear to be localized to the long arm of chromosome 7 in this hybrid clone.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D002869:Chromosome Aberrations; D002906:Chromosomes, Human, 6-12 and X; D003165:Complement System Proteins; D003601:Cytotoxicity Tests, Immunologic; D005796:Genes; D005966:Glucuronidase / Q000032:analysis; D006801:Humans; D006822:Hybrid Cells / Q000276:immunology*; D007106:Immune Sera; D007621:Karyotyping; D051379:Mice; D009374:Neoplasms, Experimental / Q000276:immunology; D012641:Selection, Genetic; D013539:Simian virus 40 / Q000276:immunology; D014178:Translocation, Genetic
D000956:Antigens, Viral; D007106:Immune Sera; D003165:Complement System Proteins; D005966:Glucuronidase