Independent modulation of hepatic protein kinase activities.
The effects of hormonal status on protein kinase activity was examined in homogenates of rat liver. Protein kinase activity was evaluated from incorporation of 32P from [gamma-32P]ATP into protamine or histone as receptor substrates. Protamine phosphorylation in the presence or absence of cyclic AMP exceeded histone phosphorylation by at least a factor or two. Hypophysectomy markedly increased protamine phosphorylation in the presence or absence of saturating amounts of cyclic AMP. In contrast, hypophysectomy only slightly increased cyclic AMP independent phosphorylation of histone. These results could not be amounted for by differences in ATPase or protein phosphase activities. Cortisone (2 mg/day x 3) decreased total protein kinase activity in livers of hypophysectomized rats when protamine was substrate, but had no effect on the total activity toward histone. Growth hormone (100 mug/day x 3) significantly increased histone, but not protamine phosphorylation in livers of hypophysectomized rats. Administration of 5 mug of triiodothyonine/day to hypophysectomized rats also markedly increased the phosphorylation of histone, but not protamine when saturating amounts of cyclic AMP were present. These results support the hypothesis that liver may contain more than one type of protein kinase activity and that the different protein kinase activities can be separately affected by hormones. Such control distal to cyclic AMP might allow selective modulation of cyclic AMP-dependent processes in cells which carry out more than one such process.
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D003348:Cortisone / Q000494:pharmacology; D000242:Cyclic AMP / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D013006:Growth Hormone / Q000494:pharmacology; D007016:Hypophysectomy; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D006656:Protamine Kinase / Q000378:metabolism; D011479:Protamines; D011494:Protein Kinases / Q000378:metabolism*; D051381:Rats; D014284:Triiodothyronine / Q000494:pharmacology
D011479:Protamines; D014284:Triiodothyronine; D013006:Growth Hormone; D000242:Cyclic AMP; D011494:Protein Kinases; D006656:Protamine Kinase; D000251:Adenosine Triphosphatases; D003348:Cortisone