title: Subcellular distribution of thiamine-pyrophosphokinase and thiamine-pyrophosphatase activities in rat isolated enterocytes.
abstract: Starting from mucosal cells, isolated from rat small intestine, subcellular fractionation was carried out. Four fractions were prepared and characterized by marker enzymes and electron microscopic examination: nuclei plus microvilli, mitochondria, microsomes and supernatant. Thiamine-pyrophosphokinase activity was localized mainly in the supernatant fraction, with minor amount in mitchondria and microsomes. On the contrary thiamine-pyrophosphatase activity was present only in the particulate fractions (especially in microsomes), being completely absent from the supernatant.
mesh_terms: D000818:Animals; D007413:Intestinal Mucosa / Q000166:cytology / Q000201:enzymology*; D008297:Male; D010770:Phosphotransferases / Q000378:metabolism*; D011755:Pyrophosphatases / Q000378:metabolism*; D051381:Rats; D013347:Subcellular Fractions / Q000201:enzymology; D013834:Thiamine Pyrophosphatase / Q000378:metabolism*
chemical_list: D010770:Phosphotransferases; D011755:Pyrophosphatases; D013834:Thiamine Pyrophosphatase