Characterization of N6, O2-dimethyladenosine from nuclear RNA of Novikoff hepatoma.
A modified nucleoside was isolated from low molecular weight nuclear RNA of Novikoff hepatoma, the nucleotide sequence of which was reported earlier. The structure of this novel nucleoside was shown to be N6, O2'-dimethyladenosine (m6Am) by mass spectrometry of its trimethylsilyl derivative and by comparison with the mass spectra of N6- and O-2' monmethyl model compounds. The complete characterization was carried out using 0.04 A260 units (2 microgram) of m6Am.
D000241:Adenosine / Q000031:analogs & derivatives*; D006528:Carcinoma, Hepatocellular / Q000032:analysis*; D002460:Cell Line; D002467:Cell Nucleus / Q000032:analysis; D008113:Liver Neoplasms; D013058:Mass Spectrometry; D009374:Neoplasms, Experimental / Q000032:analysis; D012334:RNA, Neoplasm / Q000032:analysis*
D012334:RNA, Neoplasm; D000241:Adenosine