Peroxidase activity in human granulocytes, inter- and intra-individual variations.
A kinetic method for quantitation of peroxidase activity ('myeloperoxidase', E.C. 1.11.1.7.) in human granulocytes has been evaluated. The enzymic activity was determined at 37 degrees C, PH = 7.0. Substrates: 2-methyxo-phenol (1.25 mmol/l) and H2O2 (0.1 mmol/l). When applied on granulocytes from solitary blood samples from healthy, normal subjects the detected peroxidase activity varied considerably from one person to another (CV = 40-50%). Sequential determinations of the peroxidase activity in granulocytes from selected healthy, normal subjects demonstrated substantial intra-individual variations. Analysis of variance confirmed that the intra-individual variations (CV = 36%) exceeded all other variance components involved.
D000328:Adult; D005260:Female; D006098:Granulocytes / Q000201:enzymology*; D006139:Guaiacol; D006801:Humans; D006861:Hydrogen Peroxide; D007962:Leukocytes / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D009195:Peroxidase / Q000097:blood*; D010544:Peroxidases / Q000097:blood*; D012016:Reference Values; D013223:Statistics as Topic; D013997:Time Factors
D006139:Guaiacol; D006861:Hydrogen Peroxide; D010544:Peroxidases; D009195:Peroxidase