Effect of polychlorinated biphenyls (Aroclor 1254) on liver gluconeogenic enzyme activities in embryonic and growing chickens.
Fertile Nick Chick eggs were injected with 0.05, 0.1, 0.5 and 5 p.p.m. of PCB (Aroclor 1254) on day zero and were incubated until the 14th day of incubation. Nick Chick cockerels, one day old, were fed different levels of PCB in their diet for two weeks. The levels of PCB used were 0.1, 1, 5, 100 and 200 p.p.m. Key gluconeogenic enzyme activities were measured in the liver of both experimental groups. In the liver of chick embryos and growing chickens the decrease of the activity of all key gluconeogenic enzymes, with the exception of G6P-ase in embyronic liver, and FDP-ase in that of growing chickens, was estimated. The inhibited gluconeogenesis may account for the embryonic death in eggs contaminated with PCB's.
D000818:Animals; D001140:Aroclors / Q000494:pharmacology*; D002642:Chick Embryo / Q000201:enzymology*; D002645:Chickens / Q000378:metabolism*; D006597:Fructose-Bisphosphatase / Q000378:metabolism; D005943:Gluconeogenesis / Q000187:drug effects*; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D008297:Male; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000378:metabolism; D011078:Polychlorinated Biphenyls / Q000494:pharmacology*; D011766:Pyruvate Carboxylase / Q000378:metabolism
D001140:Aroclors; D011078:Polychlorinated Biphenyls; D006597:Fructose-Bisphosphatase; D005952:Glucose-6-Phosphatase; D010729:Phosphoenolpyruvate Carboxykinase (GTP); D011766:Pyruvate Carboxylase