Nucleotide-induced inhibition of surface sialyl transferase activity on cultured Burkitt's lymphoma cells.
Sialyl transferase activity was demonstrated on the surfaces of intact, cultured lymphoblastoid cells (RAJI) derived from a Burkitt's lymphoma. Pretreatment of the cells with neuraminidase increased the labeled sialoprotein by severalfold. A nunber of nucleotides were effective in decreasing the amount of sialoprotein assembly. CMP was the most effective inhibitor. UMP, AMP, and GMP were also inhibitory, but to a lesser degree. The diphosphate derivatives were similarly inhibitory, but generally less active than their monophosphate counterparts. The cyclic nucleotides were the least effective of all nucleotides tested; cCMP and cAMP showed a small degree of activity, whereas cUMP and cGMP were without effect. These studies indicated that a number of noncyclic and cyclic nucleotides can influence the activity of the sialyl transferase system.
D000249:Adenosine Monophosphate / Q000494:pharmacology; D002051:Burkitt Lymphoma / Q000201:enzymology* / Q000378:metabolism; D002462:Cell Membrane / Q000201:enzymology; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000494:pharmacology; D006152:Cyclic GMP / Q000494:pharmacology; D003568:Cytidine Monophosphate / Q000494:pharmacology; D006150:Guanine Nucleotides / Q000494:pharmacology; D009363:Neoplasm Proteins / Q000096:biosynthesis; D009711:Nucleotides / Q000494:pharmacology*; D009712:Nucleotides, Cyclic / Q000494:pharmacology; D012795:Sialoglycoproteins / Q000096:biosynthesis; D012799:Sialyltransferases / Q000037:antagonists & inhibitors*; D014166:Transferases / Q000037:antagonists & inhibitors*; D014500:Uracil Nucleotides / Q000494:pharmacology
D006150:Guanine Nucleotides; D009363:Neoplasm Proteins; D009711:Nucleotides; D009712:Nucleotides, Cyclic; D012795:Sialoglycoproteins; D014500:Uracil Nucleotides; D000249:Adenosine Monophosphate; D000242:Cyclic AMP; D014166:Transferases; D012799:Sialyltransferases; D003568:Cytidine Monophosphate; D006152:Cyclic GMP