Inhibition of internuclear transport of SV40-induced T antigen in heterokaryons.
Internuclear migration of tumour specific nuclear T antigen has been analysed in SV40-induced H-50 tumour and chick erythrocyte heterokaryons. Thirty hours after cell fusion the incorporated and enlarged erythrocyte nuclei were invariably T antigen-positive. When treated with colchicine at 10(-4) or 10(-7) M concentration, the erythrocyte nuclei incorporated into the heterokaryons did not swell and remained T antigen-negative. The results strongly suggest the involvement of a colchicine sensitive contractile protein matrix in the internuclear transport of T antigen and other proteins.
D000818:Animals; D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D002459:Cell Fusion; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D002642:Chick Embryo; D003078:Colchicine / Q000494:pharmacology; D006224:Cricetinae; D004912:Erythrocytes / Q000648:ultrastructure*; D006579:Heterozygote; D013539:Simian virus 40; D013936:Thymidine / Q000378:metabolism
D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D003078:Colchicine; D013936:Thymidine