title: Isoenzymes of lactate dehydrogenase, acid phosphatase, alkaline phosphatase and peroxidase in monkey kidney cell cultures inoculated with herpes virus type 1.
abstract: The variation of lactate dehydrogenase, peroxidase, acid and alkaline phosphatase isoenzymes was studied in VERO cells inoculated with infectant and UV-inactivated herpes simplex virus type 1 (HSV--1). Infectant HSV--1 induced quantitative and qualitative modifications in isoenzyme patterns within the first 4 hours post inoculation (p.i.). The modifications caused by the UV-inactivated HSV--1 were similar, but appeared 8 hours p.i. The possibility of using isoenzyme modifications as rapid, sensitive and specific biochemical tests for virus detection and differentiation between infectant and inactivated virus is discussed.
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism*; D000469:Alkaline Phosphatase / Q000378:metabolism*; D002460:Cell Line; D007527:Isoenzymes / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D010544:Peroxidases / Q000378:metabolism*; D018139:Simplexvirus / Q000254:growth & development* / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase; D010544:Peroxidases; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase