Increased numbers of heat-resistnat spores produced by two strains of Clostridium perfringens bearing temperate phage s9.
Sporulation kinetics and spore heat resistance data were compared for a lysogenic strain of Clostridium perfringens, s9, before and after curing with ultraviolet irradiation. The cured strain showed the same growth rate in broth media as the lysogenic strain but took 6 h longer to form refractile spores. For lysogenized and cured strains the percentages of refractile spores produced that were heat-resistant (80 degrees C for 15 min) were 50 and 0.2, respectively. When reinfected with the temperature phage, the cured strain produced spores in 2 to 3 h, like the original lysogenic culture, and 10% of the spores produced were heat-resistnat.
D001435:Bacteriophages; D003016:Clostridium perfringens / Q000032:analysis / Q000502:physiology*; D006358:Hot Temperature; D008242:Lysogeny; D010848:Picolinic Acids / Q000032:analysis; D013171:Spores, Bacterial
D010848:Picolinic Acids