Tissue-cultured head and neck tumors: their use in in vitro assays of immune response.
Samples of 35 tumors from the head and neck region (25 squamous cell, 2 basal cell, 5 parotid, 3 melanoma, and 1 lymphosarcoma) were cultured after dispersement with either trypsin or collagenase treatment. Growth was established in 14 (40%). Cultured tumor cells were then used as target cells in in vitro assays of patients' cellular and humoral immunity to their own or similar tumors. Preliminary data suggest this may be a reliable method of monitoring responses in patients receiving immunotherapy for head and neck malignancies.
D000230:Adenocarcinoma / Q000276:immunology; D002280:Basal Cell Carcinoma / Q000276:immunology; D002294:Carcinoma, Squamous Cell / Q000276:immunology; D046508:Culture Techniques / Q000379:methods; D006258:Head and Neck Neoplasms / Q000276:immunology*; D006801:Humans; D007109:Immunity; D007111:Immunity, Cellular; D008228:Lymphoma, Non-Hodgkin / Q000276:immunology; D008545:Melanoma / Q000276:immunology; D003012:Microbial Collagenase; D014357:Trypsin
D014357:Trypsin; D003012:Microbial Collagenase