The dissociation of the surface architecture described by enhanced lectin agglutinability and the transformed phenotype expressed as anchorage independence.
Using a series of cold-sensitive variants of chemically transformed BHK-21 cells, revertants to the normal phenotype derived from a dimethyl-nitrosamine transformed clone of BHK-21 as well as revertants to the normal phenotype derived from polyoma transformed BHK-21 cells we have demonstrated that the surface phenotype described by enhanced agglutinability with Con A and WGA can be dissociated from the transformed phenotype described by anchorage independence (growth in semisolid medium). Specifically we have demonstrated that the surface characteristic of enhanced agglutinability may be found in a variety of cell lines which fail to display to grow in agar. Our work clearly shows that the two phenotypes described are not concomitantly controlled and tends to suggest that the phenotype of enhanced lectin agglutinability may be dissociated from the transformed phenotype.
D000362:Agar; D000371:Agglutination / Q000187:drug effects; D000818:Animals; D002455:Cell Division; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002999:Clone Cells / Q000276:immunology*; D003080:Cold Temperature; D003208:Concanavalin A / Q000494:pharmacology; D006224:Cricetinae; D037102:Lectins / Q000494:pharmacology*; D009602:Nitrosamines; D010641:Phenotype; D037121:Plant Lectins; D011120:Polyomavirus; D014908:Triticum
D037102:Lectins; D009602:Nitrosamines; D037121:Plant Lectins; D003208:Concanavalin A; D000362:Agar