In vitro stimulation of bovine circulating lymphocytes by parainfluenza type 3 virus.
Bovine circulating lymphocytes from animals sero-positive to parainfluenza-3 virus (PIV-3) could be stimulated by PIV-3 antigen in a microculture system. A semiautomatic multiple sample harvester was used to collect the lymphocytes from the microplates. Ultraviolet-killed PIV-3 induced better stimulation values than formalin or heat-treated virus. The highest stimulation values were obtained after a stimulation time of three to five days.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D002417:Cattle / Q000276:immunology*; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D010224:Parainfluenza Virus 3, Human / Q000276:immunology* / Q000528:radiation effects; D018108:Respirovirus / Q000276:immunology*; D014466:Ultraviolet Rays
D000914:Antibodies, Viral