Nonspecific effect of m7GMP on protein-RNA interactions.
Cap analogs m7GMP and m7GDP inhibit binding of eukaryotic initiation factors to reovirus capped mRNA but also inhibit complex formation involving uncapped mRNA or 18 S rRNA. Furthermore, Escherichia coli DNA-dependent RNA polymerase binds 18 S rRNA and this interaction is also blocked by m7GMP. These results indicate that inhibition by cap analogs is not a stringent test for putative cap-specific binding between proteins and mRNA.
D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism; D006150:Guanine Nucleotides / Q000031:analogs & derivatives*; D006153:Guanosine Diphosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D006157:Guanosine Monophosphate / Q000031:analogs & derivatives* / Q000494:pharmacology; D007700:Kinetics; D010442:Peptide Chain Initiation, Translational; D010448:Peptide Initiation Factors; D012333:RNA, Messenger / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000378:metabolism*
D006150:Guanine Nucleotides; D010448:Peptide Initiation Factors; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D006153:Guanosine Diphosphate; D006157:Guanosine Monophosphate; D012321:DNA-Directed RNA Polymerases