Turnover of cellular carbohydrates in normal and Rous sarcoma virus-transformed cells.
We have analyzed the distribution of glucosamine-labeled polymers on the cell surface, in the growth medium, and inside the cell and the net turnover of these polymers during the process of malignant transformation of chick embryo fibroblasts by Rous sarcoma virus. The distribution of label and the turnover kinetics for hyaluronic acid, total glycoproteins, and chondroitins were found to be identical in both normal and transforming cultures. We conclude that nonspecific degradative processes are probably not involved in causing transformation-related alterations in cell surface carbohydrates, althougn degradation of specific macromolecules is not excluded.
D001358:Avian Sarcoma Viruses; D050260:Carbohydrate Metabolism; D002462:Cell Membrane / Q000378:metabolism; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D002809:Chondroitin Sulfates / Q000378:metabolism; D003847:Deoxyglucose / Q000378:metabolism; D005944:Glucosamine / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism; D006025:Glycosaminoglycans / Q000378:metabolism; D006820:Hyaluronic Acid / Q000378:metabolism; D007700:Kinetics; D008565:Membrane Proteins / Q000378:metabolism; D009363:Neoplasm Proteins / Q000378:metabolism; D011108:Polymers / Q000378:metabolism
D006023:Glycoproteins; D006025:Glycosaminoglycans; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D011108:Polymers; D006820:Hyaluronic Acid; D002809:Chondroitin Sulfates; D003847:Deoxyglucose; D005944:Glucosamine