title: Structural changes accompanying the removal of pyridoxal 5'-phosphate from phosphorylase b.
abstract: The changes in physical properties accompanying the removal of pyridoxal 5'-phosphate from glycogen phosphorylase b have been examined. The apoenzyme retains a high degree of structural rigidity, as determined from the time decay of anisotropy. The bulk of the secondary structure remains intact, although a significant change in circular dichroism indicates some degree of alteration. The mobility of a sulfhydryl-linked spin label increases. The restoration of pyridoxal 5'-phosphate reverses this effect, with indication of interaction between subunits. One or more new binding sites for 1-anilinonaphthalene-8-sulfonate appear for the apoenzyme. The kinetics of the recombination of pyridoxal 5'-phosphate with the apoenzyme, as monitored by difference spectra, indicate a high activation energy for the process. The apoenzyme is a reversibly associating system at 20-30 degrees C, pH 7.0.
mesh_terms: D000818:Animals; D001051:Apoenzymes / Q000378:metabolism; D001059:Apoproteins / Q000378:metabolism; D001665:Binding Sites; D002151:Calorimetry; D002942:Circular Dichroism; D004578:Electron Spin Resonance Spectroscopy; D005453:Fluorescence; D006005:Phosphorylases / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D011732:Pyridoxal Phosphate / Q000378:metabolism; D011817:Rabbits; D013053:Spectrophotometry; D013113:Spin Labels; D013329:Structure-Activity Relationship; D013696:Temperature
chemical_list: D001051:Apoenzymes; D001059:Apoproteins; D013113:Spin Labels; D011732:Pyridoxal Phosphate; D006005:Phosphorylases