Epidermal adenylate cyclase systems: the retention of hormone responsiveness after enzymatic separation of pure epidermis.
Although it has been shown that keratome-sliced skin contains active adenylate cyclase systems which respond to various hormones and drugs, unequivocal proof that the epidermis contains these hormone-responsive systems is still lacking. We demonstrate in this study that "pure" epidermis obtained after either collagenase or trypsin treatment does contain the hormone-sensitive adenylate cyclase systems.
D000249:Adenosine Monophosphate / Q000494:pharmacology; D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D001965:Bromides / Q000008:administration & dosage; D000242:Cyclic AMP / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D004837:Epinephrine / Q000494:pharmacology; D006632:Histamine / Q000494:pharmacology; D006652:Histological Techniques; D006728:Hormones / Q000494:pharmacology*; D003012:Microbial Collagenase / Q000008:administration & dosage; D009638:Norepinephrine / Q000494:pharmacology; D011956:Receptors, Cell Surface / Q000187:drug effects; D012867:Skin / Q000187:drug effects / Q000201:enzymology*; D013552:Swine; D014357:Trypsin / Q000008:administration & dosage
D001965:Bromides; D006728:Hormones; D011956:Receptors, Cell Surface; D000249:Adenosine Monophosphate; D006632:Histamine; D000242:Cyclic AMP; D014357:Trypsin; D003012:Microbial Collagenase; D000262:Adenylyl Cyclases; D009638:Norepinephrine; D004837:Epinephrine