[Advantages of the Eagle medium over saline solutions in the studies of the metabolic parameters of tumor cells in vitro].
In two media (krebsringer phosphate and phosphate-enriched Eagle medium) the indices of energetic metabolism (respiration, glycolysis. ATP content) were determined and the metabolism of macromolecular compounds (c-RNA) of Ehrlich carcinoma ascitic cells was investigated. The results of studies of ascites tumor cells metabolism have indicated considerable advantages of phosphate-enriched Eagle medium over krebs ringer phosphate buffer.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000473:pathology*; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D003470:Culture Media; D003593:Cytoplasm / Q000378:metabolism*; D005069:Evaluation Studies as Topic; D006019:Glycolysis; D066298:In Vitro Techniques; D007552:Isotonic Solutions; D008113:Liver Neoplasms / Q000473:pathology*; D051379:Mice; D009374:Neoplasms, Experimental / Q000473:pathology; D010101:Oxygen Consumption; D010710:Phosphates; D012334:RNA, Neoplasm / Q000378:metabolism; D012462:Saline Solution, Hypertonic; D012996:Solutions
D003470:Culture Media; D007552:Isotonic Solutions; D010710:Phosphates; D012334:RNA, Neoplasm; D012462:Saline Solution, Hypertonic; D012996:Solutions; D000255:Adenosine Triphosphate
Preimushchestva sredy Igla pered solevymi restvorami pri izuchenii in vitro metabolicheskikh parametrov opukholevykh kletok.