T1 oligonucleotides that segregate with tropism and with properties of gp70 in recombinants between N- and B-tropic murine leukemia viruses.
We have analyzed large RNase T1-resistant oligonucleotides derived from the genomes of 16 recombinants between N- and B-tropic murine leukemia viruses of BALB/c. The parental viruses, designated SP-N and LP-B, differ in several phenotypic or biochemically defined properties: N- or B-tropism; XC plaque morphology, electrophoretic mobility of three virion proteins (p15, p30, and gp70); ability to induce GIX antigen on infected cells; presence of 6 to 8 (out of 36 to 38 analyzable) large T1 oligonucleotides. One SP-N-specific T1 oligonucleotide was inherited by all 16 N-tropic recombinants and, thus, appears to be linked to N-tropism. This oligonucleotide lies in the 5' third of the oligonucleotide map of SP-N. One LP-B-specific T1 oligonucleotide was inherited by all 11 recombinants whose gp70  has an electrophoretic mobility like that of LP-B gp70 and that, like LP-B, fail to induce GIX antigen. This oligonucleotide lies in the 3' third of the oligonucleotide map of LP-B.
D000818:Animals; D002460:Cell Line; D005814:Genes, Viral; D009052:Leukemia Virus, Murine / Q000032:analysis* / Q000235:genetics / Q000254:growth & development; D051379:Mice; D008815:Mice, Inbred Strains; D009841:Oligonucleotides / Q000032:analysis*; D012367:RNA, Viral / Q000032:analysis*; D011995:Recombination, Genetic; D014779:Virus Replication
D009841:Oligonucleotides; D012367:RNA, Viral