Comparison of vesicular stomatitis virus defective interfering particle synthesis in chick embryo and L cells.
A comparison of the ability of vesicular stomatitis virus (VSV) to generate and replicate defective interfering (DI) particles in primary chick embryo (CE) and mouse L cells was investigated as a means of analyzing host control over DI-particle synthesis and interfering capacity. Serial undiluted passage of VSV in CE and L cells indicate that VSV-DI particles are generated and (or) replicate with greater efficiency in CE than in L cells. When DI particles accumulate in L cells, they are able to interfere with infectious particle replication. The DI particles from CE cells interfered to the same extent with infectious particle replication in both CE and L cells. L cells, therefore, are not considered 'low-interference' hosts in which DI particles are produced and do not interfere with infectious virus replication, but rather hosts which restrict the production of DI particles.
D000818:Animals; D002642:Chick Embryo; D046508:Culture Techniques; D003673:Defective Viruses / Q000254:growth & development*; D007739:L Cells; D014721:Vesicular stomatitis Indiana virus / Q000254:growth & development*; D014762:Viral Interference; D014779:Virus Replication