Cellular interactions uncouple beta-adrenergic receptors from adenylate cyclase.
C6 glioma cells and B104 neuroblastoma cells both possess adenylate cyclase activity, but only C6 cells have beta-adrenergic receptors. However, when cocultured with B104 cells, C6 cells show a marked decrease in their ability to accumulate adenosine 3', 5'-monophosphate upon stimulation with beta receptor agonists. Since both beta receptors and cholera toxin-stimulated adenylate cyclase activities are present in C6/B104 cocultures, we conclude that the beta receptor/adenylate cyclase transduction mechanism in cocultured C6 cells is uncoupled.
D000262:Adenylyl Cyclases / Q000378:metabolism*; D002450:Cell Communication; D002460:Cell Line; D002772:Cholera Toxin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D008565:Membrane Proteins / Q000378:metabolism; D011485:Protein Binding; D011941:Receptors, Adrenergic / Q000378:metabolism*; D011943:Receptors, Adrenergic, beta / Q000378:metabolism*
D008565:Membrane Proteins; D011941:Receptors, Adrenergic; D011943:Receptors, Adrenergic, beta; D002772:Cholera Toxin; D000242:Cyclic AMP; D000262:Adenylyl Cyclases