title: The neuronal origin of prostaglandin released from the rabbit portal vein in response to electrical stimulation.
abstract: Transmural electrical stimulation of the isolated portal vein of the rabbit was accompanied by the release of a prostaglandin-like substance (PLS). Thin layer chromatography coupled with bioassay indicated that this substance was probably prostaglandin E2 (PGE2). 2 Indomethacin potentiated the response of the portal vein to electrical stimulation at 2 Hz and abolished the release of the PLS. 3 There was no significant change in the amount of PLS released from the portal vein in response to electrical stimulation at 2 Hz when the contractile response of the portal vein was prevented by pretreatment with phentolamine or guanthidine. 4 In vitro denervation of the portal vein with 6-hydroxydopamine or the omission of Ca2+ from the bathing solution caused a significant reduction in the amount of PLS released from the portal vein in response to electrical stimulation at 2 hertz. 5 It is concluded that electrical stimulation of the isolated portal vein of the rabbit is accompanied by the release of a PLS, probably PGE2, from a neuronal source. The synthesis and release of the PLS is Ca2+ -dependent.
mesh_terms: D000818:Animals; D002118:Calcium / Q000494:pharmacology; D003714:Denervation; D004558:Electric Stimulation; D006145:Guanethidine / Q000494:pharmacology; D066298:In Vitro Techniques; D007213:Indomethacin / Q000494:pharmacology; D008297:Male; D009469:Neuromuscular Junction / Q000502:physiology*; D009489:Neurosecretion; D010646:Phentolamine / Q000494:pharmacology; D011169:Portal Vein; D011453:Prostaglandins / Q000378:metabolism*; D011817:Rabbits; D009435:Synaptic Transmission
chemical_list: D011453:Prostaglandins; D002118:Calcium; D007213:Indomethacin; D010646:Phentolamine; D006145:Guanethidine