Expression of early virus functions in human cytomegalovirus infected HEL cells: effect of ultraviolet light-irradiation of the virus.
Ultraviolet (u.v.) light-irradiation of human cytomegalovirus (HCMV) resulted in differential inactivation of virus capacities, e.g. induction of cell rounding, early antigens (EA), nuclear inclusion, HCMV DNA synthesis, cellular DNA synthesis, HCMV-specific DNA polymerase, cellular DNA polymerases and plaque production, while the capacity of HCMV to penetrate cell nuclei was not critically impaired. These results indicated that the virus-coded functions expressed after infection were responsible for sll these events except for HCMV-induced stimulation of cellular RNA synthesis which was enhanced by irradiation of the virus at a low dose of u.v. light (6600 ergs/mm2). In these experiments phosphonoacetic acid was effectively utilized to detect EA formation by immunofluorescent staining and to differentiate cellular DNA synthesis from virus DNA synthesis.
D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D002467:Cell Nucleus / Q000382:microbiology; D003587:Cytomegalovirus / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects*; D004279:DNA, Viral / Q000096:biosynthesis; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D007181:Inclusion Bodies, Viral; D010746:Phosphonoacetic Acid / Q000494:pharmacology; D012313:RNA / Q000096:biosynthesis; D014466:Ultraviolet Rays; D014779:Virus Replication
D000956:Antigens, Viral; D004279:DNA, Viral; D012313:RNA; D004259:DNA-Directed DNA Polymerase; D010746:Phosphonoacetic Acid