title: Strandedness of Pichinde virus RNA.
abstract: The Pichinde virus RNA did not possess the following characteristics of eucaryotic mRNA: polyadenylic acid sequence, capped methylated structure, and ability to direct protein synthesis in vitro. Polysomal RNA extracted from cells infected with Pichinde virus reannealed with 32P-labeled virus RNA, protecting about 60% of the latter against RNase degestion. The polyadenylic acid-containing polysomal RNA also reannealed to the 32P-labeled virus RNA to approximately the same extent. These indicate that the major part of the genomic RNA of Pichinde virus is negative stranded.
mesh_terms: D006477:Arenaviruses, New World / Q000032:analysis*; D001483:Base Sequence; D046508:Culture Techniques; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000032:analysis; D011132:Polyribosomes / Q000032:analysis; D012328:RNA Viruses / Q000032:analysis*; D012367:RNA, Viral / Q000032:analysis
chemical_list: D012367:RNA, Viral; D011061:Poly A