[Different cooperativity of some oxidoreductases to specialized and nonspecialized regulators].
The activation of 2 different mouse liver enzymes: cytozolic disulfide reductase (DSR) and mitochondrial NAD-isocitrate dehydrogenase (ICDH), by catecholamines and especially by 3',5'-AMP is characterized by negative cooperativity; substrate (both enzymes), protamine and EDTA (DSR) produce the positive cooperativity type of activation; DSR activation by isopropyl noradrenaline and serotonine is characterized by hyperbolic kinetics. Consequently, one and the same enzyme can combine positive cooperativity to non-specialized regulators (substrate, protamine, EDTA) with negative cooperativity to specialized regulators (3',5'-AMP, catecholamines). The systems, switching on by catecholamines and 3',5'-AMP, are oligomeric, and the degree and even the type of cooperativity can modify depending on the kind of catecholamine. The negative cooperativity is revealed in literature for many effects of catecholamines and 3',5'-AMP. Probably, it guarantees the broad range of regulations. Dose effect curves for 3',5'-AMP, catecholamines and other hormones should be analyzed on the basis of allosteric protein kinetics. A simple nomogram is given to estimate nH less than 1.
D000494:Allosteric Regulation; D000818:Animals; D002395:Catecholamines / Q000494:pharmacology; D000242:Cyclic AMP / Q000494:pharmacology; D003600:Cytosol / Q000201:enzymology; D004220:Disulfides; D004305:Dose-Response Relationship, Drug; D004492:Edetic Acid / Q000494:pharmacology; D004789:Enzyme Activation; D007521:Isocitrate Dehydrogenase / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D046911:Macromolecular Substances; D008433:Mathematics; D051379:Mice; D008930:Mitochondria, Liver / Q000201:enzymology; D010088:Oxidoreductases / Q000378:metabolism*; D011479:Protamines / Q000494:pharmacology
D002395:Catecholamines; D004220:Disulfides; D046911:Macromolecular Substances; D011479:Protamines; D004492:Edetic Acid; D000242:Cyclic AMP; D010088:Oxidoreductases; D007521:Isocitrate Dehydrogenase
Raznaia kooperativnost' nekotorykh oksidoreduktaz po otnosheniiu k spetsializirovannym i nespetsializirovannym reguliatoram.