Particulate' hexokinase activity in rat intestine. The comparative contributions of mitochondria and brush-border membranes.
The activity of hexokinase was examined in brush-border membranes purified from rat intestine. Compared with homogenates, purified membranes exhibited a 20-fold increase in sucrase specific activity and a 15-fold decrease in hexokinase specific activity, which argues against the structural localization of hexokinase within the brush-border membrane.
D000818:Animals; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D006593:Hexokinase / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000201:enzymology*; D008297:Male; D008861:Microsomes / Q000201:enzymology; D008928:Mitochondria / Q000201:enzymology; D051381:Rats; D013347:Subcellular Fractions / Q000201:enzymology; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013393:Sucrase / Q000378:metabolism
D013385:Succinate Dehydrogenase; D006593:Hexokinase; D005952:Glucose-6-Phosphatase; D013393:Sucrase