title: Suppressor cell regulation of immune response to tumors: abrogation by adult thymectomy.
abstract: The regulatory role of the adult thymus on the appearance of cytotoxic and suppressor T cells (thymus-derived lymphocytes) to allogeneic and autochthonous virus-induced tumors in mice was investigated. It was demonstrated that C57BL/6 mice challenged with allogeneic P815 mastocytoma cells and complete Freund's adjuvant failed to develop cytotoxic cells but instead developed suppressor T cells which inhibited cytotoxic T cell function. Further, adjuvant-induced suppressor cells prevented the primary in vitro induction of cytotoxic T cells to P815 mastocytoma cells. In contrast, adult thymectomized animals, when challenged with adjuvant and allogeneic cells, had a normal cytotoxic response in vivo and their cells could not inhibit the generation of cytotoxic T cells in vitro. These studies suggested that the intact adult thymus was necessary for the induction of suppressor cells. Moreover, suppressor cells regulated cytotoxic T cell activity both in vivo and in vitro. Further, it was shown that adjuvant could prevent the normal immune response to virus-induced tumors. BALB/c mice treated with murine sarcoma virus developed tumors which reached a maximal size by day 14 and then regressed. Sham thymectomized animals treated with virus and complete Freund's adjuvant to generate suppressor cells died from progressive tumor growth. In contrast, thymectomized animals similarly treated had normal regression of tumor and survived. These studies lead to the conclusion that the adult thymus may regulate immune responsiveness by the export of suppressor T cells which regulate other T cell responses to both allogeneic and tumor antigens.
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm; D003601:Cytotoxicity Tests, Immunologic; D005620:Freund's Adjuvant; D006649:Histocompatibility Antigens; D007111:Immunity, Cellular; D007165:Immunosuppression Therapy; D051379:Mice; D008815:Mice, Inbred Strains; D012208:Rhabdomyosarcoma / Q000276:immunology; D009053:Sarcoma Viruses, Murine; D012513:Sarcoma, Experimental / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D013934:Thymectomy; D013950:Thymus Gland / Q000276:immunology*
chemical_list: D000951:Antigens, Neoplasm; D006649:Histocompatibility Antigens; D005620:Freund's Adjuvant