title: Cytochemical and biochemical studies of yeasts after in vitro exposure to miconazole.
abstract: Yeast cells exposed to different doses of the antimycotic agent miconazole revealed important cytochemical changes in the topographic distribution of the phosphatases. A strong effect was observed on the behavior of oxidative and peroxidative enzymes. Decreased cytochrome c oxidase and peroxidase activity and increased catalase activity were seen after treatment with a fungistatic dose of miconazole, whereas a complete disappearance of these enzymes was observed after treatment with a minimal fungicidal dose of miconazole. This was in complete agreement with the quantitative biochemical data. A hypothesis is advanced concerning the possible involvement of peroxidase and catalase in the mechanism of action of this drug.
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D002176:Candida albicans / Q000187:drug effects / Q000201:enzymology; D002374:Catalase / Q000378:metabolism; D003578:Cytochrome-c Peroxidase; D003576:Electron Transport Complex IV / Q000378:metabolism; D007093:Imidazoles / Q000494:pharmacology*; D008825:Miconazole / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000201:enzymology; D013997:Time Factors; D015003:Yeasts / Q000187:drug effects*
chemical_list: D007093:Imidazoles; D008825:Miconazole; D003578:Cytochrome-c Peroxidase; D002374:Catalase; D003576:Electron Transport Complex IV; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase