title: Characterization of Ia antigens in mouse serum.
abstract: Sera obtained from normal B10.BR mice were shown to inhibit selectively a specific anti-Ia alloantiserum. Partial purification of the Ia antigenic activity was accomplished by isolation of the high density lipoproteins from these sera by fractional precipitation with sodium phosphotungstate and MgCL2. Both H-2.23 and Iak antigens present in this high density lipoprotein fraction were completely adsorbed by rabbit anit-rat beta2-microglobulin immunoadsorbents, whereas specific anti-H-2.23 immunoadsorbents removed only the H-2 activity. These data deomnstrate that Ia antigens, like H-2 antigens in the sera of B10.BR mice are associated with high density lipoproteins and further suggest that both H-2 and Ia antigens are associated with a beta2-microglobulin-like molecule.
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D005591:Chemical Fractionation; D003429:Cross Reactions; D006649:Histocompatibility Antigens / Q000032:analysis*; D007164:Immunosorbents; D007519:Isoantigens / Q000032:analysis*; D008075:Lipoproteins, HDL / Q000032:analysis; D051379:Mice; D008815:Mice, Inbred Strains; D001613:beta 2-Microglobulin
chemical_list: D006649:Histocompatibility Antigens; D007164:Immunosorbents; D007519:Isoantigens; D008075:Lipoproteins, HDL; D001613:beta 2-Microglobulin