The reduced extracellular calcium requirement for proliferation by neoplastic hepatocytes.
Cells from neonatal rat livers were unable to maintain DNA-synthetic activity in calcium-deficient medium, but neoplastic hepatocytes from Morris hepatomas 5123 tc and 7795 synthesized DNA and proliferated indefinitely in this calcium-deficient medium. The calcium content of fresh hepatoma tissue from which these cultures were derived was as much as 10 times greater than that of normal liver; but this difference could not account for the insensitivity of neoplastic cells to extracellular calcium because it disappeared during subsequent cultivation in vitro.
D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D006528:Carcinoma, Hepatocellular; D002455:Cell Division / Q000187:drug effects; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D008099:Liver / Q000166:cytology*; D008113:Liver Neoplasms; D051381:Rats
D004247:DNA; D002118:Calcium