[Adenylate kinase and creatine kinase activity of rabbit blood serum following application of a tourniquet to the thigh].
A colorimetric method is described for estimation of the adenilate kinase activity in blood serum; the method is based on coupling of adenilate- and creatine kinase reactions and on estimation of the amount of creatine formed. Adenilate kinase activity in blood serum, estimated by the method, was shown to increase 4-fold after removing of tourniquet with subsequent normalization within the next day. The same data were obtained using a spectrophotometric method for estimation of adenilate kinase based on NADP reduction in coupled reactions with hexokinase and glucose-6-phosphate dehydrogenase. An increase in creatine kinase activity in blood serum occurred later on after removing of the tourniquet; it was more distinct (8.5-fold) and maintained longer as compared with the increase in adenilate kinase activity.
D000263:Adenylate Kinase / Q000097:blood*; D000818:Animals; D003402:Creatine Kinase / Q000097:blood*; D009132:Muscles / Q000098:blood supply; D009135:Muscular Diseases / Q000097:blood* / Q000201:enzymology; D010770:Phosphotransferases / Q000097:blood*; D011817:Rabbits; D013848:Thigh; D014111:Tourniquets
D010770:Phosphotransferases; D003402:Creatine Kinase; D000263:Adenylate Kinase
Adenilatkinaznaia i kreatinkinaznaia aktivnost' syvorotki krovi krolikov posle nalozheniia zhguta na bedro.