Properties of density gradient-fractionated peripheral blood leukocytes from cattle infected with bovine leukemia virus.
Discontinuous bovine serum albumin gradients were used to fractionate peripheral blood leukocytes from bovine leukemia virus (BLV)-free and BLV-infected cows. The release of infectious BLV and spontaneous incorporation of [3H]thymidine were not properties of density gradient-fractionated leukocytes from a BLV-free cow. When leukocytes from BLV-infected cattle were fractionated, B lymphocytes which spontaneously incorporated [3H]thymidine could be separated as a distinct subpopulation from B lymphocytes which replicated infectious BLV. Density gradient fractionation of leukocytes from a cow with lymphosarcoma is also reported. A fall in lymphocyte count at the time of tumor development is attributed to the loss of B lymphocytes which spontaneously incorporate [3H]thymidine.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D001402:B-Lymphocytes / Q000378:metabolism; D002417:Cattle; D002499:Centrifugation, Density Gradient; D007104:Immune Adherence Reaction; D001909:Leukemia Virus, Bovine / Q000276:immunology; D007962:Leukocytes / Q000276:immunology / Q000378:metabolism; D008218:Lymphocytosis / Q000097:blood* / Q000209:etiology; D008228:Lymphoma, Non-Hodgkin / Q000097:blood* / Q000209:etiology; D012190:Retroviridae / Q000276:immunology; D013936:Thymidine / Q000378:metabolism
D000956:Antigens, Viral; D013936:Thymidine