title: Isolation and purification of reaction center from Rhodopseudomonas viridis NHTC 133 by means of LDAO.
abstract: Two different procedures are described to isolate and purify the reaction center complex from Rhodopseudomonas viridis NHTC 133 by means of the non-ionic detergent dodecyldimethylamine oxide. Both reaction center particles thus obtained were active, as shown by a photobleaching centered at 975 nm. The reaction center also contained, in addition to bacteriochlorophyll, bacteriopheophytin. Other components were also found in this particle: cytochromes C553 and C558 and a menaquinone-like substance. The SDS gel electrophoresis of reaction centers is shown. The molecular weights of the subunits forming the reaction center in 0.5% sodium dodecyl sulfate and 1% mercaptoethanol were calculated as being: 45 +/- 1.5 and 37 +/- 1.5 kdalton, 29 +/- 1.5 and 23 +/- 1.5 kdalton. The molecular weight of the complex determined by means of gel filtration (Sepharose 6-B and Bio-Gel P-300) gives a value of approximately 240 kdalton. The minimum molecular weight of the complex calculated by disc gel electrophoresis was 231 kdalton.
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis; D001429:Bacteriochlorophylls / Q000032:analysis; D002458:Cell Fractionation / Q000379:methods; D003574:Cytochrome c Group / Q000032:analysis; D003902:Detergents; D008970:Molecular Weight; D010674:Pheophytins / Q000032:analysis; D010788:Photosynthesis; D011809:Quinones / Q000032:analysis; D012241:Rhodopseudomonas / Q000032:analysis
chemical_list: D001426:Bacterial Proteins; D001429:Bacteriochlorophylls; D003574:Cytochrome c Group; D003902:Detergents; D010674:Pheophytins; D011809:Quinones