[Proliferation of L 929 mouse fibroblasts and synthesis of active peptidyl prolyl hydroxylase in these cells in monolayer culture under the influence of D-penicillamine, azathioprine, aurothiopolypeptide, oxyphenbutazone and prednisolone (author's transl)].
L 929 mouse fibroblasts were cultured as monolayer using MEM Eagle medium after addition of bovine serum albumin, antibiotics, antimycotica, ferrous nitrate and ascorbic acid. In 24 hours distances cell number was counted. At the same time peptidyl prolyl hydroxylase activity was determined and related to the protein concentration in the cells. The proliferation of the cells followed a typical curve. The cell number decreased after trypsination, then increased logarithmically, finally reached a stationary phase. Enzyme activity showed a correspondent curve. Active enzyme did not appear until near the end of the log phase. After addition of the mesenchyme active substances in therapeutical concentrations proliferation and synthesis of active peptidyl prolyl hydroxylase in the cells were significantly decreased. The results are discussed in relation to the characteristics of collagen synthesis and proliferation of connective tissue cells under physiological, pathophysiological and therapeutical conditions.
D000818:Animals; D001379:Azathioprine / Q000494:pharmacology; D002455:Cell Division; D002460:Cell Line; D003238:Connective Tissue / Q000187:drug effects*; D005347:Fibroblasts / Q000187:drug effects / Q000201:enzymology*; D006046:Gold / Q000494:pharmacology; D051379:Mice; D010113:Oxyphenbutazone / Q000494:pharmacology; D010396:Penicillamine / Q000494:pharmacology; D010455:Peptides / Q000494:pharmacology; D011239:Prednisolone / Q000494:pharmacology; D011393:Procollagen-Proline Dioxygenase / Q000096:biosynthesis
D010455:Peptides; D006046:Gold; D011239:Prednisolone; D011393:Procollagen-Proline Dioxygenase; D010396:Penicillamine; D010113:Oxyphenbutazone; D001379:Azathioprine
Der teinfluss vonD-Penicillamin, Azathioprin, Aurothiopolypeptid, Oxyphenbutazon und Prednisolon auf die Proliferation von L 929-Mäusefibroblasten und die Synthese aktiver tpeptidylprolyhydroxylase in diesen Zellen in der Monolayerkultur.