title: The interaction of Clostridium perfringens and its toxins in the production of necrotic enteritis of chickens.
abstract: The intraduodenal administration of large numbers of Clostridium perfringens cells harvested from broth cultures and resuspended in PBS or fresh sterile thioglycollate broth produced a very mild form of necrotic enteritis. Administering an appropriate number of cells in culture supernatant, however, produced typical field-type disease. Alpha toxin was shown to be the significant toxin recoverable from broth-culture supernatant fluids. Requirements to produce the disease are minor intestinal damage and sufficient numbers of toxigenic C. perfringens in the intestine.
mesh_terms: D000818:Animals; D000992:Antitoxins; D002645:Chickens; D003015:Clostridium Infections / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D003016:Clostridium perfringens / Q000254:growth & development; D004386:Duodenum / Q000473:pathology; D004530:Egg Yolk; D004751:Enteritis / Q000209:etiology / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D005260:Female; D007583:Jejunum / Q000473:pathology; D008297:Male; D009336:Necrosis; D011201:Poultry Diseases / Q000209:etiology* / Q000382:microbiology / Q000473:pathology; D014118:Toxins, Biological
chemical_list: D000992:Antitoxins; D014118:Toxins, Biological