Lowered respiratory activity in hypo-osmotically shocked mitochondria without loss of cytochrome c.
Brief exposure of rat liver mitochondria to hypo-osmotic sucrose media caused a decline in the rates of succinate and ascorbate-NNN'N'-tetramethyl-p-phenylenediamine oxidation without loss of cytochrome c when assayed in iso-osmotic media. Lowered respiration rates in mitochondria after brief exposure to hypo-osmotic media may reflect a modification of cytochrome c binding.
D000818:Animals; D003470:Culture Media; D003574:Cytochrome c Group; D005260:Female; D008930:Mitochondria, Liver / Q000378:metabolism*; D009994:Osmolar Concentration; D010101:Oxygen Consumption; D011189:Potassium Chloride; D051381:Rats; D013386:Succinates / Q000378:metabolism; D013395:Sucrose; D013772:Tetramethylphenylenediamine / Q000378:metabolism
D003470:Culture Media; D003574:Cytochrome c Group; D013386:Succinates; D013395:Sucrose; D011189:Potassium Chloride; D013772:Tetramethylphenylenediamine