Determination of the mass of viruses by quantitative electron microscopy.
The photometric method of quantitative determination of dry mass by electron microscopy has been applied to the study of various types of viruses: animal, plant, insect, and bacterial. The method is applicable to all viruses having a mass of 1 x 10-18g or greater. The molecular weight of viruses can be calculated from the mass value by multiplying it by Avogadro's number. In comparison to other methods of determining the molecular weight of viruses, sedimentation and diffusion, sedimentation equilibrium, light scattering, and electron microscopy counting, the method of quantitative electron microscopy is competitive. In some ways quantitative electron microscopy is superior to other methods for the determination of molecular weight: There is no limitation to the size of the virus, no experimental time involved and no concentration and purity of virus preparations required, and finally it is independent of the geometry of the virion. In one important aspect it is unique when compared to other methods; namely, it affords one the capacity to analyse individual virus particles.
D055592:Biophysical Phenomena; D001703:Biophysics; D003090:Coliphages / Q000648:ultrastructure; D005069:Evaluation Studies as Topic; D007304:Insect Viruses / Q000648:ultrastructure; D008854:Microscopy, Electron / Q000379:methods*; D008970:Molecular Weight; D010783:Photometry; D010942:Plant Viruses / Q000648:ultrastructure; D013539:Simian virus 40 / Q000648:ultrastructure; D018139:Simplexvirus / Q000648:ultrastructure; D014027:Tobacco Mosaic Virus / Q000648:ultrastructure; D014616:Vaccinia virus / Q000648:ultrastructure; D014780:Viruses / Q000648:ultrastructure*