title: Evidence that B-lymphocytes carry the nuclear pocket abnormality associated with bovine leukemia virus infection.
abstract: Peripheral blood lymphocytes from 3 cows with persistent lymphocytosis were separated on nylon wool columns into nylon wool-adherent and nonadherent populations. The percentage of cells coated with surface immunoglobulin (B-cells) and the frequency of lymphocytic nuclear pockets in each sub-population were then determined. In each case the adherent population consisted predominantly of B-cells with an increased nuclear pocket frequency, whereas the nonadherent cells were 98.99% negative for surface immunoglobulin (non-B-cells) and contained essentially no nuclear pockets. These findings provided additional evidence that the B-subpopulation of cells was highly involved in bovine leukemia oncogenesis.
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology / Q000382:microbiology / Q000648:ultrastructure*; D002417:Cattle; D002418:Cattle Diseases / Q000209:etiology / Q000276:immunology / Q000382:microbiology; D002469:Cell Separation; D005260:Female; D007938:Leukemia / Q000209:etiology / Q000276:immunology / Q000382:microbiology / Q000648:ultrastructure / Q000662:veterinary*; D001909:Leukemia Virus, Bovine; D008214:Lymphocytes / Q000648:ultrastructure; D009685:Nuclear Envelope / Q000648:ultrastructure; D011947:Receptors, Antigen, B-Cell; D012190:Retroviridae; D014412:Tumor Virus Infections / Q000473:pathology*
chemical_list: D011947:Receptors, Antigen, B-Cell