Human rotavirus and its antibody: their coexistence in feces of infants.
Rotavirus and its antibody were detected by paper disk solid-phase radioimmunoassay or electron microscopy in feces of infants and young children with acute diarrhea. The fecal specimens in which rotavirus was detectable often contained a high titer of antibodies, which were shown by radioimmunoassay to belong mainly to the immunoglobulin G class. Rotavirus was rarely detected in the specimens containing immunoglobulin A antibodies. By dissociation tests carried out by radioimmunoassay, it was shown that the rotavirus particles in some specimens had the same antibody-binding capacity as did cultured simian rotavirus (SA-11), but antibodies in feces usually had low avidity, probably resulting from enzymatic digestion.
D000914:Antibodies, Viral / Q000032:analysis*; D001666:Binding Sites, Antibody; D002675:Child, Preschool; D003126:Colostrum / Q000276:immunology; D003968:Diarrhea, Infantile / Q000276:immunology / Q000382:microbiology*; D005243:Feces / Q000276:immunology / Q000382:microbiology*; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007223:Infant; D012328:RNA Viruses / Q000302:isolation & purification*; D011863:Radioimmunoassay; D012401:Rotavirus / Q000276:immunology / Q000302:isolation & purification*
D000914:Antibodies, Viral; D007070:Immunoglobulin A; D007074:Immunoglobulin G