Simian virus 40-specific proteins in the membranes of simian virus 40-transformed hamster and mouse cells.
Membranes of simian virus 40-transformed hamster lymphocytes and phagocytes, as well as of transformed mouse fibroblasts, contain two classes of antigenic virus-specific protein. The isoelectric points of these proteins, as defined by isoelectric focusing/immune electrophoresis are at pH 4.5 and 4.7. The molecular weights of the pI 4.5 and pI 4.7 components, determined by isoelectric focusing/dodecyl sulfate polyacrylamide electrophoresis, lie near 58,000 and 90,000-110,000, respectively. The pI 4.5 and pI 4.7 proteins are tentatively identified with the surface (transplantation) and U antigens, respectively.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002462:Cell Membrane / Q000032:analysis; D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D006224:Cricetinae; D004591:Electrophoresis, Polyacrylamide Gel; D007122:Immunoelectrophoresis; D007525:Isoelectric Focusing; D008565:Membrane Proteins / Q000032:analysis*; D008647:Mesocricetus; D008970:Molecular Weight; D013539:Simian virus 40 / Q000032:analysis*; D014764:Viral Proteins / Q000032:analysis*
D000956:Antigens, Viral; D008565:Membrane Proteins; D014764:Viral Proteins