title: Cleavage of native type III collagen in the collagenase susceptible region by thermolysin.
abstract: Viscometric assays were used to demonstrate the activity of thermolysin (EC 3.4.24.4) on native type III collagen in solution. Analysis of the reaction products by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and electron microscopic visualisation of segment long spacing aggregates demonstrated localised cleavage of the collagen in the collagenase susceptible region.
mesh_terms: D003094:Collagen / Q000378:metabolism*; D003012:Microbial Collagenase / Q000378:metabolism; D008970:Molecular Weight; D013379:Substrate Specificity; D013381:Subtilisins / Q000378:metabolism; D013820:Thermolysin / Q000378:metabolism*; D014357:Trypsin / Q000378:metabolism
chemical_list: D003094:Collagen; D013381:Subtilisins; D014357:Trypsin; D013820:Thermolysin; D003012:Microbial Collagenase