[Enzymatic micromethod for determining Na, K-ATPase activity in rat cerebral cortex membranes].
A method for the assay of Na,K-ATPase activity of unpurified synaptosomal fraction obtained from the microquantities (2--3 mg of fresh tissue) of the rat cerebral cortex is described. This method is based on the fluorimetric determination of ADP formed in the course of ATPase reaction. The method is highly sensitive and may be used to determine the membrane preparations Na,K-ATPase activity with the protein content of 0.05--10.0 microgram per sample.
D000818:Animals; D002540:Cerebral Cortex / Q000201:enzymology* / Q000648:ultrastructure; D008722:Methods; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis*; D013574:Synaptosomes / Q000201:enzymology
D000254:Sodium-Potassium-Exchanging ATPase
Fermentativnyĭ mikrometod opredeleniia aktivnosti Na, K-ATF-azy v membranakh kory golovnogo mozga krys.