title: [Immunofluorescence with PI-3 virus--preparation and evaluation of conjugates from immune sera of various animal species].
abstract: Immune sera with a high content of antibodies to para-influenza-3 (PI-3) virus suitable for production of specific conjugated immunoglobulins were prepared on conventionally reared calves, rabbits, and guinea-pigs, applying antigen in different ways. When processing the sera of the above mentioned animal species to conjugates, the sera differed in pure IgG yield, in completed conjugate, and molar F/P ratios, but their ability to demonstrate antigens of the PI-3 virus in the dilution was approximately the same. The serum prepared on a colostrum deprived calf showed the lowest antibody content and the lowest pure IgG yield, the colouring ability of the conjugate prepared being as well somewhat lower.
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000302:isolation & purification; D002417:Cattle; D005455:Fluorescent Antibody Technique / Q000379:methods*; D006168:Guinea Pigs; D007106:Immune Sera / Q000302:isolation & purification*; D007074:Immunoglobulin G / Q000302:isolation & purification; D010224:Parainfluenza Virus 3, Human / Q000276:immunology*; D011817:Rabbits; D018108:Respirovirus / Q000276:immunology*; D013045:Species Specificity
chemical_list: D000914:Antibodies, Viral; D007106:Immune Sera; D007074:Immunoglobulin G
vernacular_title: Imunofluorescencia s vírusom PI-3--príprava a vyhodnotenie konjugátov z imúnnych sér viacerých druhov zvierat.