Proteins of bovine leukemia virus. I. Characterization and reactions with natural antibodies.
The bovine leukemia virus (BLV) was purified from a chronically infected fetal lamb kidney cell line. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of this virus revealed the presence of eight distinguishable viral components with molecular weights ranging from 80,000 to 11,000. The major component is a non-glycosylated protein having a molecular weight of 24,000 (p24). At least three heavier polypeptides were found, one of them representing a glycoprotein (gp 60). In addition, four minor polypeptides with respective molecular weights of 19,000, 16,000, 13,000, and 11,000 were identified. In a complement fixation assay using naturally occurring antibodies of a leukemic cow, four polypeptides, which included gp 60, p35, p24, and p16, were found to be reactive.
D000914:Antibodies, Viral; D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D003168:Complement Fixation Tests; D006023:Glycoproteins / Q000032:analysis; D001909:Leukemia Virus, Bovine / Q000032:analysis* / Q000276:immunology; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis / Q000276:immunology; D012190:Retroviridae / Q000032:analysis*; D014764:Viral Proteins / Q000032:analysis* / Q000276:immunology
D000914:Antibodies, Viral; D000956:Antigens, Viral; D006023:Glycoproteins; D010455:Peptides; D014764:Viral Proteins