[Separation of enriched synaptosomes, synaptic vesicles and synaptic plasma membranes].
A rapid and simple method is described for separation of intact synaptosomes, synaptic plasma membranes and vesicles. Two synaptosome fractions were obtained by modified differential centrifugation. The rate zonal zentrifugation in a linear sucrose gradient (very low density) is suitable to obtain fractions highly enriched in synaptic plasma membranes and vesicles. Examination of the prepared fractions was done by enzyme marker activities and electron microscopy
D000251:Adenosine Triphosphatases / Q000032:analysis; D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D002499:Centrifugation, Density Gradient; D008274:Magnesium; D008854:Microscopy, Electron; D009247:NADH, NADPH Oxidoreductases / Q000032:analysis; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis; D013570:Synaptic Membranes / Q000648:ultrastructure*; D013572:Synaptic Vesicles / Q000648:ultrastructure*; D013574:Synaptosomes / Q000648:ultrastructure*
D009247:NADH, NADPH Oxidoreductases; D000251:Adenosine Triphosphatases; D000254:Sodium-Potassium-Exchanging ATPase; D008274:Magnesium
Gewinnung von angereicherten Synaptosomen, synaptischen Membranen und Vesikeln.