Isolation and characterization of nonhistone chromosomal protein C-14 which stimulates RNA synthesis.
The nonhistone chromatin protein, C-14, was extracted from chromatin of Novikoff hepatoma ascites cells and isolated in high purity as shown by its migration as a single dense spot on two-dimensional polyacrylamide gels. Its mobility on sodium dodecyl sulfate gels is consistent with a molecular weight of approximately 70 000. The amino acid composition shows that protein C-14 has an acidic:basic amino acid ratio of 1.8. Its amino terminal amino acid is lysine. Protein C-14 stimulated the incorporation of [3H]UMP into RNA by approximately 30% when added to naked DNA and homologous RNA polymerase I. A 30% stimulation of [3H]UMP incorporation into RNA was also found when protein C-14 was added to an E. coli RNA polymerase system containing either E. coli or Novikoff hepatoma DNA.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D006528:Carcinoma, Hepatocellular / Q000378:metabolism; D002466:Cell Nucleolus / Q000032:analysis; D002467:Cell Nucleus / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D002843:Chromatin / Q000302:isolation & purification; D002850:Chromatography, Gel; D002868:Chromosomal Proteins, Non-Histone / Q000302:isolation & purification* / Q000494:pharmacology / Q000502:physiology; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology; D066298:In Vitro Techniques; D008113:Liver Neoplasms / Q000378:metabolism; D008970:Molecular Weight; D009374:Neoplasms, Experimental / Q000378:metabolism; D012318:RNA Polymerase I / Q000302:isolation & purification; D012334:RNA, Neoplasm / Q000096:biosynthesis*
D000596:Amino Acids; D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D012334:RNA, Neoplasm; D012318:RNA Polymerase I