[Cell cultures obtained using collalytin from the pancreases of cattle fetuses].
The authors present morphological characteristics of primary monolayer cultures prepared from the pancreas of bovine fetuses. Combined treatment with trypsin and collalytine solutions (a preparation with collagenase activity) was used for dispersion of the tissue of the pancreas. Numerous epithelial cells corresponding by morphofunctional characteristics to beta-cells of the islets of Langerhans were contained in be cultures obtained; an aldehyde-fuchsin-positive granularity was revealed in the cytoplasm of these cells. Degranulation of these cells occurred under the effect of an increased glucose concentration in the nutrient medium.
D000818:Animals; D002417:Cattle; D002469:Cell Separation; D002478:Cells, Cultured; D007515:Islets of Langerhans / Q000196:embryology*; D003012:Microbial Collagenase; D014357:Trypsin
D014357:Trypsin; D003012:Microbial Collagenase
Kletochnye kul'tury iz podzheldudochnoĭ zhelezy plodov krupnogo rogatogo skota, poluchennye s primeneniem kollalitina