title: Temperature-sensitive mutants of human cytomegalovirus.
abstract: Eight temperature-sensitive mutants of human cytomegalovirus have been isolated after mutagenesis with nitrosoguanidine. Three of these mutants have been classified into three separate complementation groups and are capable of synthesizing virus DNA at the nonpermissive temperature (39.5 degrees C). Two others appear unable to synthesize virus DNA at the elevated temperature.
mesh_terms: D003587:Cytomegalovirus / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D004279:DNA, Viral / Q000096:biosynthesis; D005816:Genetic Complementation Test; D006801:Humans; D008769:Methylnitronitrosoguanidine; D009153:Mutagens; D009154:Mutation; D013696:Temperature; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D009153:Mutagens; D008769:Methylnitronitrosoguanidine