Interaction of pigeon-liver nicotinamide-adenine dinucleotide kinase with cibacron blue F3GA.
The interaction of pigeon liver NAD kinase with Cibacron Blue F3GA was investigated. By using steady-state rate measurements, spectrophotometric titration and chromatography of the enzyme on immobilized dye, it was shown that binding occurs at two nucleotide sites with different affinities, and also at a site distinct from the substrate-binding region.
D000818:Animals; D001665:Binding Sites; D004396:Coloring Agents; D010856:Columbidae; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D010770:Phosphotransferases
D004396:Coloring Agents; D010770:Phosphotransferases