Photooxidation of chlorophyll in spinach chloroplasts between 10 and 180 K.
Electron paramagnetic resonance (EPR) and optical absorbance difference spectra and kinetics upon illumination by saturating flashes and continuous light of spinach chloroplasts frozen under various conditions were measured between 10 and 180 K. 1. At 100 K illumination with continuous light caused an EPR signal which decayed during the light in about 30 ms. This change is probably due to the reduction of P+-680, the oxidized primary electron donor of Photosystem II, by a secondary electron donor, cytochrome b-559. Flash illumination yielded the previously observed rapid (2 ms) transient. This transient has been ascribed to a back-reaction of the two primary reagents of Photosystem II (Malkin, R. and Bearden, A.J. (1975) Biochim. Biophys. Acta 396, 250-259; Visser, J.W.M. (1975) Thesis, Leiden). 2. Between 10 and 40 K, illumination with continuous light showed a transient which decayed in about 500 ms. The extent decreased with increasing temperature. However, the half time appeared to be temperature independent. This signal was also attributed to P+-680. 3. At 180 K it appeared to be impossible to observe the 2 and 30 ms components in dark frozen chloroplasts. However, they could be observed again if two short saturating flashes were given shortly before freezing. These changes seem to be dependent on the S-state of the reaction center. 4. After oxidizing the sample with ferricyanide (Eh = 540 mV), the light induced absorbance difference spectrum showed a bleaching near 676 nm. This change is ascribed to the irreversible oxidation of a dimeric chlorophyll molecule which acts as electron donor to P+-680 under these conditions. 5. Titration curves of the irreversible light-induced absorbance change at 676 nm and the irreversible light-induced EPR change near g = 2.00 provide strong evidence that these two changes reflect the same compound. Finally, a model is given to explain the observed reactions of Photosystem II at 10-180 K. The model involves three different ultimate and one intermediate electron donor to P+-680 at these temperatures.
D002734:Chlorophyll / Q000378:metabolism*; D002736:Chloroplasts / Q000187:drug effects / Q000378:metabolism*; D003624:Darkness; D004237:Diuron / Q000494:pharmacology; D004578:Electron Spin Resonance Spectroscopy; D005615:Freezing; D007700:Kinetics; D008027:Light; D010944:Plants; D013053:Spectrophotometry
D002734:Chlorophyll; D004237:Diuron