Separation of subclasses of human serum high density lipoproteins by zonal ultracentrifugation.
An improved one-step method for the preparative separation of three subfractions of high-density lipoproteins from normal human serum has been developed. It employs the method of rate zonal ultracentrifugation in a z-60 rotor using a discontinuous NaBr gradient in the density range of 1.0-1.4. The density gradients were monitored directly by a flow-through density meter allowing the direct read-out of the actual densities in the process of filling and emptying the rotor. The separation of the three density fractions from 5 to 15 ml serum was achieved during a single 12 hours run at 59.000 rpm. The three fractions showed characteristically different patterns on polyacrylamide gel electrophoresis and differences in their lipid and protein composition.
D002501:Centrifugation, Zonal / Q000379:methods; D005260:Female; D006801:Humans; D008075:Lipoproteins, HDL / Q000032:analysis / Q000097:blood* / Q000302:isolation & purification; D008297:Male
D008075:Lipoproteins, HDL