Evidence of agar gel electrophoresis changes of lipoprotein-X after phospholipase A and deoxycholic acid.
The effect of phospholipase A from snake venom and deoxycholic acid on lipoprotein-X (LP-X) recovered from the cathode side of a previous agar gel electrophoresis is described. Adding phospholipase A and deoxycholic acid to the removed cathodal fraction is followed by a marked migration to the anode side on a second electrophoresis procedure. This seems to confirm that phospholipase A and bile salts on LP-X particles modifying their agar gel electrophoretic migration characteristics.
D002779:Cholestasis / Q000097:blood; D003840:Deoxycholic Acid / Q000494:pharmacology*; D004587:Electrophoresis, Agar Gel; D006801:Humans; D008077:Lipoproteins, LDL / Q000097:blood*; D010740:Phospholipases / Q000494:pharmacology*
D008077:Lipoproteins, LDL; D003840:Deoxycholic Acid; D010740:Phospholipases