title: Defective interfering particles with covalently linked [+/-]RNA induce interferon.
abstract: Defective interfering (DI) particles of vesicular stomatitis virus which contain covalently linked complementary [+]message and [-]anti-message RNA as a single-stranded ribonucleoprotein complex within the particle, are extremely efficient inducers of interferon. A single particle can induce a quantum yield of interferon. A single molecule of double-stranded RNA presumed to form, at least in part, on entry into the cell is thought to induce interferon synthesis. Conventional [-]RNA DI particles with the same polypeptide composition as [+/-]RNA DI particles fail to induce interferon.
mesh_terms: D000914:Antibodies, Viral; D000937:Antigen-Antibody Reactions; D002460:Cell Line; D002470:Cell Survival; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D003673:Defective Viruses / Q000378:metabolism* / Q000528:radiation effects; D006358:Hot Temperature; D007372:Interferons / Q000096:biosynthesis*; D009690:Nucleic Acid Conformation; D012367:RNA, Viral / Q000378:metabolism*; D013698:Templates, Genetic; D014466:Ultraviolet Rays; D014721:Vesicular stomatitis Indiana virus / Q000276:immunology / Q000378:metabolism* / Q000528:radiation effects; D014762:Viral Interference
chemical_list: D000914:Antibodies, Viral; D012367:RNA, Viral; D003609:Dactinomycin; D007372:Interferons; D003513:Cycloheximide