Relation of cell type and cell density in tissue culture to the isoaccepting spectra of the nucleoside Q containing tRNAs: tRNATyr, tRNAHis, tRNAAsn and tRNAAsp.
An examination, using reversed-phase chromatography and cyanogen bromide treatment, of tRNATyr, tRNAHis, tRNAAsn, and tRNAAsp from SV40-transformed mouse fibroblasts grown to different cell densities, untransformed cells grown to confluence, and mouse liver indicates that: (1) The tissue cultured mouse fibroblasts examined here are hypomodified with respect to nucleoside Q, while liver tRNA is almost completely modified with respect to Q. (2) Cell density and/or proliferative state do not present as major variables in controlling the expression of Q in the present system. (3) SV40 virus transformation is not a major variable controlling the expression of Q in the present system. The present results support previous use of cyanogen bromide effected shifts in chromatographic elution as an assay for nucleoside Q.
D001216:Asparagine; D001224:Aspartic Acid; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000378:metabolism*; D003260:Contact Inhibition; D003488:Cyanogen Bromide; D006151:Guanosine / Q000031:analogs & derivatives* / Q000378:metabolism; D006639:Histidine; D012343:RNA, Transfer / Q000378:metabolism*; D013539:Simian virus 40; D014443:Tyrosine
D006151:Guanosine; D001224:Aspartic Acid; D014443:Tyrosine; D006639:Histidine; D001216:Asparagine; D012343:RNA, Transfer; D003488:Cyanogen Bromide