The effect of gamma radiation and neocarzinostatin on NAD and ATP levels in mouse leukaemia cells.
When mouse leukemia cells are treated with gamma-radiation or neocarzinostatin the intracellular NAD and ATP levels fall rapidly. We have shown that the ATP response is a consequence of the decreased NAD level. We suggest that this low NAD level results in decreased glycolytic activity and that there is a subsequent accumulation of phosphorylated sugars associated with the fall in ATP. Under these extreme conditions, therefore, the NAD level probably regulates the rate of glycolysis in cells which are utilising a rapidly metabolisable sugar as their energy source.
D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005720:Gamma Rays; D006019:Glycolysis / Q000187:drug effects / Q000528:radiation effects*; D007939:Leukemia L1210 / Q000378:metabolism*; D009243:NAD / Q000378:metabolism*; D009536:Niacinamide / Q000031:analogs & derivatives / Q000494:pharmacology; D013936:Thymidine / Q000494:pharmacology; D009353:Zinostatin / Q000494:pharmacology*
D000903:Antibiotics, Antineoplastic; D009243:NAD; D009536:Niacinamide; D000255:Adenosine Triphosphate; D009353:Zinostatin; D013936:Thymidine