Human cytomegalovirus: purification of enveloped virions and dense bodies.
Enveloped virions and dense bodies of human cytomegalovirus have been purified by centrifugation, using combination negative viscosity: positive density gradients. Light-scattering bands of each component were obtained, and when these were examined by immune electron microscopy minimal cross contamination was observed.
D002460:Cell Line; D002499:Centrifugation, Density Gradient; D003587:Cytomegalovirus / Q000302:isolation & purification* / Q000648:ultrastructure; D006801:Humans; D007181:Inclusion Bodies, Viral