Properties of the H-4-II-E tumor cell system. I. Growth and cell proliferation kinetics of an experimental hepatoma.
Growth and cell proliferation kinetics of hepatoma H-4-II-E and its tissue culture derivative have been studied to establish the characteristics of an in vivo--in vitro solid tumor model. The H-4-II-E line, originating from the Reuber H-35 hepatoma, can be maintained and studied either in cell culture or as a transplantable solid tumor in ACI male rats. In addition it allows for the in vitro assay of cell survival following treatment of animal tumors in situ. In vivo, hepatoma H-4-II-E is rapidly growing tumor with a mean doubling time of 49-2 hr. The cell cyle time is 39-1 hr with a cell loss factor of 0-32. Retrospective examination of tumor specimens obtained during the establishment of the H-4-II-E tumor system demonstrates that both structural as well as cell population changes have occurred. The biological characteristics of the primary tumor (H-35) and an early intermediate stage (H-35tc2) are compared with H-4-II-E and the histopathological, growth and cell kinetic changes are discussed.
D015073:2-Acetylaminofluorene / Q000008:administration & dosage; D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000139:chemically induced / Q000473:pathology*; D002455:Cell Division; D002460:Cell Line; D002470:Cell Survival; D004195:Disease Models, Animal; D007700:Kinetics; D008113:Liver Neoplasms; D008297:Male; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000139:chemically induced; D051381:Rats; D011912:Rats, Inbred ACI
D015073:2-Acetylaminofluorene