Purification of simian virus 40 tumor antigen from a line of simian virus 40-transformed human cells.
Simian virus 40 tumor antigen can be isolated in a highly purified state from the nuclei ofSV80 cells, a continuous line of simian virus 40-transformed human fibroblasts. A five-step purification method was used. Its apparent molecular weight (in sodium dodecyl sulfate/polyacrylamide gels) is approximately 90,000-94,000. It contains a detectable amino-terminal residue.
D000596:Amino Acids / Q000032:analysis; D000956:Antigens, Viral / Q000302:isolation & purification*; D002460:Cell Line; D002472:Cell Transformation, Viral; D006801:Humans; D008970:Molecular Weight; D011233:Precipitin Tests; D013539:Simian virus 40 / Q000276:immunology*
D000596:Amino Acids; D000956:Antigens, Viral