Effects of X-irradiation on the hybridization of rat thymus nuclear RNA with repeated and unique DNA sequences.
The kinetics of DNA hybridization with heterogeneous nuclear RNA (hnRNA) from normal and phytohaemagglutinin (PHA)-stimulated thymocytes has been studied in control rats and in animals 30 min after exposure to whole-body X-radiation with 400 rad. Irradiation results in a diminished ability of hnRNA to form hybrids with DNA at all C0t values ranging from 10(-3) to 10(4). Since this effect is most pronounced in the regions of low repetitive and unique DNA sequences, it is concluded that whole-body X-irradiation of animals may also suppress the transcription, particularly, in these regions.
D000818:Animals; D001483:Base Sequence; D006528:Carcinoma, Hepatocellular; D004247:DNA / Q000528:radiation effects*; D004273:DNA, Neoplasm / Q000528:radiation effects; D037102:Lectins / Q000494:pharmacology; D008113:Liver Neoplasms; D008213:Lymphocyte Activation; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental; D009693:Nucleic Acid Hybridization / Q000528:radiation effects*; D012313:RNA / Q000528:radiation effects*; D051381:Rats; D013601:T-Lymphocytes / Q000187:drug effects / Q000528:radiation effects*; D014965:X-Rays
D004273:DNA, Neoplasm; D037102:Lectins; D012313:RNA; D004247:DNA