Purification of hepatitis A virus from chimpanzee stools.
Hepatitis A virus antigen was purified from early acute-phase chimpanzee stools by a rapid three-step procedure using 7% polyethylene glycol precipitation, CsCl banding, and Sepharose 2B column chromatography. Electron microscopic examination of the hepatitis A virus entigen preparation revealed highly purified hepatitis A virus particles.
D000818:Animals; D000956:Antigens, Viral / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology*; D006507:Hepatovirus / Q000276:immunology / Q000302:isolation & purification* / Q000648:ultrastructure; D002679:Pan troglodytes; D011863:Radioimmunoassay
D000956:Antigens, Viral