title: Temperature-sensitive replication of H5ts125 adenovirus DNA in vitro.
abstract: A soluble adenovirus DNA replication system has been prepared in extracts of HeLa cells infected with the temperature-sensitive mutant H5ts125. These nuclear extracts synthesize full-sized viral DNA at 30 degrees but are temperature sensitive in this function at 38 degrees. A 72,000-dalton DNA-binding protein purified from cells infected with wild-type virus complements the temperature-sensitive defect and restores viral DNA replication to 74% of normal values.
mesh_terms: D000260:Adenoviruses, Human / Q000235:genetics*; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D002474:Cell-Free System; D004265:DNA Helicases / Q000235:genetics / Q000378:metabolism*; D004279:DNA, Viral / Q000096:biosynthesis*; D005796:Genes; D005814:Genes, Viral; D009154:Mutation; D013696:Temperature; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D004265:DNA Helicases