Intracellular precursors to the major glycoprotein of avian oncoviruses in chicken embryo fibroblasts.
A 96,000-dalton glycoprotein, p(96), was present in cell extracts obtained from gs-chf- chicken embryo fibroblasts infected with the avian RNA tumor viruses Rous-associated virus-2 subgroup B (RAV-2) and the Schmidt-Ruppin strain of Rous sarcoma virus subgroup A (SR-RSV-A), as well as from uninfected gsLchf+ (HE) cell extracts. It was not found in cell extracts from uninfected gs-chf- or gs+chf+ (HH) cells, nor from gs-chf- cells infected with envelope-deficient Bryan high-titer Rous sarcoma virus. Immunoprecipitation, kinetic, and biochemical data indicate the this polyprotein contains information that gives rise to the major virion glycoprotein gp85. A second polyprotein of 80,000 daltons, p/80), is also present in the RAV-2- and SR-RSV-A-infected gs-chf- cells. This second polyprotein contains less carbohydrate than p(96), and kinetic and biochemical data indicate that p(80) may be an immature form of p(96).
D000818:Animals; D001354:Avian Leukosis Virus / Q000032:analysis*; D001358:Avian Sarcoma Viruses / Q000032:analysis*; D002642:Chick Embryo; D046508:Culture Techniques; D005347:Fibroblasts; D006023:Glycoproteins / Q000032:analysis* / Q000276:immunology; D008970:Molecular Weight; D011498:Protein Precursors / Q000032:analysis* / Q000276:immunology; D014764:Viral Proteins / Q000032:analysis* / Q000276:immunology
D006023:Glycoproteins; D011498:Protein Precursors; D014764:Viral Proteins