A two-step enzymatic formation of a glucoprotein in potato tuber.
Evidence is presented on the occurrence of a two-step mechanism for the synthesis of protein bound glucan in a potato tuber particulate preparation. Experiments carried out with a differently labeled sugar donor for each step enabled the isolation of a double-labeled glucoprotein. Smith periodate degradation of the product confirmed this hypothesis.
D000245:Adenosine Diphosphate Glucose / Q000378:metabolism; D005958:Glucosephosphates / Q000378:metabolism; D005964:Glucosyltransferases / Q000378:metabolism; D006023:Glycoproteins / Q000096:biosynthesis*; D007700:Kinetics; D010084:Oxidation-Reduction; D010504:Periodic Acid; D010944:Plants / Q000201:enzymology; D014532:Uridine Diphosphate Glucose / Q000378:metabolism
D005958:Glucosephosphates; D006023:Glycoproteins; D010504:Periodic Acid; D000245:Adenosine Diphosphate Glucose; D005964:Glucosyltransferases; D014532:Uridine Diphosphate Glucose