Adenosine 3',5'-monophosphate in plasma, urine, and native and isoproterenol-stimulated leukocytes of dogs.
Adenosine 3',5'-monophosphate (cAMP) was determined by means of a commercial kit in urine and plasma from 40 hospitalized dogs and in native and isoproternol-stimulated leukocytes from 30 of these animals. Mean urine and plasma concentrations were 6.1 micron and 14 nM, with 95% tolerance limits ("normal values") ranging from 0.0 to 12 micron and 0.21 to 27 nM, respectively. The plasma concentration was approximately half the value previously reported for experimental dogs. The median cAMP content in native leukocytes was 5.9 pmol/10(7) cells. The mean response to isoproterenol was 0.85 pmol/10(7) cells, much less than in human leukocytes. The response was statistically significant (P less than 0.01), but was so small that it is unlikely to be measurably affected in atopic dogs.
D000818:Animals; D000242:Cyclic AMP / Q000097:blood / Q000378:metabolism* / Q000652:urine; D004285:Dogs / Q000378:metabolism*; D007545:Isoproterenol / Q000494:pharmacology*; D007962:Leukocytes / Q000187:drug effects / Q000378:metabolism*
D000242:Cyclic AMP; D007545:Isoproterenol