Target tissues for relaxin identified in vitro with 125I-labelled porcine relaxin.
Various tissues from the mouse, rat and guinea-pig were used to examine the binding of a biologically active, esterified and 125I-labelled porcine relaxin. Binding to mouse symphysial homogenates was time- and temperature-dependent. Other peptide hormones did not complete with relaxin for binding. Mouse uterine tissue displayed similar binding characteristics. Fractionated mammary tissue from 15- and 20-day-pregnant rats exhibited significant relaxin binding activity, as did homogenates of the guinea-pig public symphysis and cervix. Under the conditions used, no relaxin receptors were noted in the liver, spleen or heart from any of the species investigated.
D000818:Animals; D005260:Female; D006168:Guinea Pigs; D008321:Mammary Glands, Animal / Q000378:metabolism; D051379:Mice; D011631:Pubic Symphysis / Q000378:metabolism; D051381:Rats; D011956:Receptors, Cell Surface / Q000378:metabolism*; D012065:Relaxin / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors; D014599:Uterus / Q000378:metabolism
D011956:Receptors, Cell Surface; D012065:Relaxin