Integrated viral DNA sequences in Epstein-Barr virus-converted human lymphoma lines.
Most human lymphoid cell lines contain multiple copies of circular, nonintegrated Epstein-Barr virus (EBV) DNA molecules as well as viral DNA sequences with properties of integrated DNA. The physical state of the EBV DNA in a human lymphoma line that only contains one virus genome equivalent per cell has now been studied by three different methods, neutral CsCl density gradient centrifugation, actinomycin D-CsCl gradient centrifugation, and Hirt fractionation. This cell line, AW-Ramos, has been obtained by EBV infection in vitro of the apparently EBV-negative Ramos lymphoma line. The results indicate that the EBV DNA in AW-Ramos is present exclusively in a linearly integrated form. Similar data were obtained with two other EBV-converted sublines of Ramos cells.
D001483:Base Sequence; D002051:Burkitt Lymphoma; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D005591:Chemical Fractionation; D004270:DNA, Circular / Q000032:analysis; D004273:DNA, Neoplasm / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis*; D004854:Herpesvirus 4, Human / Q000032:analysis*
D004270:DNA, Circular; D004273:DNA, Neoplasm; D004279:DNA, Viral