Large-scale production, concentration and purification of Rous Sarcoma Virus in tissue culture.
A method is described for long-term standard production of purified virus from cultures of PR-RSV-C-transformed chicken cells. The mean yields were 4.91 mg of purified virus per liter of culture medium. Cells were grown in long-term cultures for 26 weeks. A combination of polyethylene glycol 6 000 precipitation and zonal centrifugation has been found satisfactory for virus concentration and purification.
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000254:growth & development / Q000302:isolation & purification*; D002478:Cells, Cultured; D011232:Chemical Precipitation; D002642:Chick Embryo; D003470:Culture Media; D008722:Methods; D011092:Polyethylene Glycols
D003470:Culture Media; D011092:Polyethylene Glycols