Effect of human leucocyte migration inhibitory factor (LIF) on 3', 5'-cyclic AMP levels of peripheral blood leucocytes.
The possible involvement of 3', 5'-cyclic AMP (cyclic AMP) in the mechanism of action of leucocyte migration inhbitory factory (LIF) was tested. LIF-treated human peripheral blood leucocytes were incubated at 37 degrees C for various times between 0 and 22 h. The concentrations of cyclic AMP in these cultures did not differ from those in controls. Furthermore, LIF did not affect the cellular release of cyclic AMP. Neither when testing purified neutrophils, which are the prime targets of LIF action, an effect of LIF was found. Cyclic AMP levels decreased with time of incubation, whether testing mononuclear cells (92 per cent lymphocytes), purified neutrophils (98 per cent), or buffy coat cells (72 per cent neutrophils; 22 per cent lymphocytes). However, a transient and as yet unexplained increase in the cyclic AMP levels of the latter mixed population of cells was manifested within 10 to 30 min of incubation.
D002478:Cells, Cultured; D000242:Cyclic AMP / Q000097:blood*; D006801:Humans; D007962:Leukocytes / Q000378:metabolism*; D008263:Macrophage Migration-Inhibitory Factors / Q000494:pharmacology*; D009504:Neutrophils / Q000378:metabolism
D008263:Macrophage Migration-Inhibitory Factors; D000242:Cyclic AMP