Evaluation of enzyme-multiplied immunoassay technique (EMIT) for determination of serum digoxin.
Sixty-seven digoxin-containing sera were analyzed by both radioimmunoassay and EMIT. After some important modifications of the EMIT method, agreement between the two methods was very good. Reproducibility of the EMIT assay was excellent; daily variations in values found for control sera were quite small, and recovery of added digoxin was good. Slight hemolysis hadnegligible effects, but highly hemolyzed specimens gave low recoveries of digoxin.
D004077:Digoxin / Q000097:blood*; D005069:Evaluation Studies as Topic; D005954:Glucosephosphate Dehydrogenase; D006801:Humans; D007124:Immunoenzyme Techniques; D008832:Microchemistry; D009243:NAD; D011863:Radioimmunoassay / Q000379:methods; D013053:Spectrophotometry
D009243:NAD; D004077:Digoxin; D005954:Glucosephosphate Dehydrogenase