Analysis of the RNA species isolated from defective particles of vesicular stomatitis virus.
Serial high multiplicity passage of a cloned stock of vesicular stomatitis virus was found to generate defective interfering particles containing three size classes of RNA, with sedimentaiton coefficients of 31 S, 23 S and 19 S. The 31 S and 23 S RNA species were found to be complementary to both the 12 to 18 S and 31 S size classes of VSV mRNAs. The 19 S class of RNA was found to be partially base-paired. All three RNA species were found to contain ppAp at their 5' termini.
D000227:Adenine Nucleotides / Q000032:analysis; D001483:Base Sequence; D002460:Cell Line; D003673:Defective Viruses / Q000032:analysis*; D009690:Nucleic Acid Conformation; D012367:RNA, Viral / Q000032:analysis*; D014721:Vesicular stomatitis Indiana virus / Q000032:analysis*
D000227:Adenine Nucleotides; D012367:RNA, Viral