Isolation of apparently wild strains of poliovirus type 1 from sewage in the Ottawa area.
In the first 4 months of 1974, 140 gauze pad samples of sewage collected in the Ottawa area were analysed by the BS-C-1 cell system for the presence of viruses pathogenic for humans. Viruses were isolated from 111 (79%) of the samples. Of the 72 (65%) isolates identified by serology and electron microscopic examination, 56 (78%) were reoviruses and 16 (22%), enteroviruses. The enterovirus isolates included one coxsackievirus B4, one vaccine strain of poliovirus type 3, nine vaccine strains of poliovirus type 1 and five strains of poliovirus type 1 that proved by serodifferentiation and temperature marker tests to be different from vaccine strains. The fact that these strains were present in the community sewage in readily detectable concentrations at a time when immunity against polioviruses is declining in such communities is a cause for concern.
D006801:Humans; D009864:Ontario; D011051:Poliomyelitis / Q000382:microbiology; D017955:Poliovirus / Q000302:isolation & purification*; D012722:Sewage; D013048:Specimen Handling / Q000379:methods; D014865:Waste Disposal, Fluid; D014871:Water Microbiology
D012722:Sewage