Photochemical attachment of cyclic AMP binding protein(s) to the nuclear genome.
The nature of the cyclic AMP-receptor-nucleus interactions was examined by a novel combination of two photoreactions. A photosensitive derivative of cyclic AMP, N6-butyryl cyclic AMP, was covalently attached to its cytoplasmic receptod by photo-affinity labelling and this receptor complex was photo-crosslinked by the DNA in the rat liver nuclei. The photolytic reactions seemed to be specific since stable links were formed only when substantial noncovalent binding occured.
D000345:Affinity Labels; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D002474:Cell-Free System; D000242:Cyclic AMP / Q000031:analogs & derivatives / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D004247:DNA / Q000378:metabolism*; D003852:Deoxyribonucleoproteins / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000648:ultrastructure; D009698:Nucleoproteins / Q000378:metabolism*; D010777:Photochemistry; D051381:Rats; D011953:Receptors, Cyclic AMP / Q000378:metabolism*
D000345:Affinity Labels; D003852:Deoxyribonucleoproteins; D009698:Nucleoproteins; D011953:Receptors, Cyclic AMP; D004247:DNA; D000242:Cyclic AMP