The effect of shearing of XC DNA on its RSV transfecting activity.
XC DNA fragments having the average molecular weight of 10(7) daltons were obtained by hydrodynamic shearing followed by sucrose gradient centrifugation or BUdR treatment of XC cells used for DNA preparation. Such DNA molecules, even after long-term storage, were found to be active in transfection.
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000378:metabolism*; D002460:Cell Line; D004273:DNA, Neoplasm / Q000378:metabolism*; D009368:Neoplasm Transplantation; D051381:Rats; D012513:Sarcoma, Experimental / Q000378:metabolism
D004273:DNA, Neoplasm