Repertoire of antiviral antibodies expressed by somatic cell hybrids.
Fusion between P3 x 63 Ag8 mouse myeloma cells and spleen cells from BALB/c mice immunized with influenza type A or B or parainfluenza type 1 virus generated reproducibly antiviral antibody-producing somatic cell hybrids (hybridomas). Eleven hybridomas derived from spleen cells of mice immunized with influenza type A virus were directed against the viral hemagglutinin, one reacted with a host component derived from chickens, and one expressed a specificity not further characterized. The hybridoma antibodies tended to be highly specific for the hemagglutinin of the immunizing virus and seemed to express the same repertoire of strain-specific antibody reactivities as splenic precursor B cells, they did not express any of the frequently occurring crossreactive anti-hemagglutinin specificities. Hybridomas producing crossreactive antibodies against hemagglutinin could be obtained if priming and boosting virus were heterologous.
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D002999:Clone Cells / Q000276:immunology; D006389:Hemagglutinins, Viral; D006822:Hybrid Cells / Q000276:immunology*; D051379:Mice; D009194:Myeloma Proteins / Q000276:immunology*; D009975:Orthomyxoviridae / Q000276:immunology*; D010223:Parainfluenza Virus 2, Human / Q000276:immunology; D013154:Spleen / Q000276:immunology*
D000914:Antibodies, Viral; D006389:Hemagglutinins, Viral; D009194:Myeloma Proteins