Effect of immunization on the cyclic AMP level and 3H-thymidine incorporation in cultured lymphoid cells.
The cyclic AMP level and the uptake of 3H-thymidine were studied in short-term suspension cultures of guinea pig lymphoid cells. Spleen cells from animals immunized 3 days previously with typhoid vaccine were shown to differ in three respects from the normal cells: (1) The level of cyclic AMP was higher. (2) The cyclic AMP increase following addition of isoproterenol or adenosine was abolished. (3) Isoproterenol (10(-5) to 10(-4) M) stimulated the uptake of 3H-thymidine in cells from immunized animals, while the uptake into normal cells was inhibited by these concentrations. A higher dose of isoproterenol (10(-3) M) inhibited 3H-thymidine uptake in both immunized and normal cells. The results reveal certain biochemical alterations in splenic lymphocytes after immunization. An altered 3H-thymidine uptake may be caused by the modified cyclic AMP metabolism in cells from immunized animals.
D000241:Adenosine / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism*; D004247:DNA / Q000378:metabolism*; D006168:Guinea Pigs; D007114:Immunization; D007545:Isoproterenol / Q000494:pharmacology; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism*; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D014436:Typhoid-Paratyphoid Vaccines
D014436:Typhoid-Paratyphoid Vaccines; D014316:Tritium; D004247:DNA; D000242:Cyclic AMP; D000241:Adenosine; D007545:Isoproterenol; D013936:Thymidine