title: Simplified method for determination of protocollagen proline hydroxylase.
abstract: Preparation of (14C) Pro labeled protocollagen and requirements for its hydroxylation by PPH was investigated. Protocollagen can be prepared by centrifugation of the biological material at 15.000 X g. Substances in current use, viz catalase, bovine serum albumin and dithiothreitol were found unnecessary under the hydroxylation conditions used. A decrease in PPH in the testis and skin of rats with increasing age was demonstrated.
mesh_terms: D000818:Animals; D001205:Ascorbic Acid; D001842:Bone and Bones; D002642:Chick Embryo; D005296:Ferrous Compounds; D008297:Male; D008722:Methods; D011347:Procollagen / Q000032:analysis*; D011393:Procollagen-Proline Dioxygenase / Q000378:metabolism; D051381:Rats; D012867:Skin / Q000201:enzymology; D013053:Spectrophotometry; D013737:Testis / Q000201:enzymology
chemical_list: D005296:Ferrous Compounds; D011347:Procollagen; D011393:Procollagen-Proline Dioxygenase; D001205:Ascorbic Acid