Culture of human cells obtained with DNA from chick Rous sarcoma.
An infectious process was reproduced in the culture of chick embryo cells by means of DNA isolated from Rous chick sarcoma tissue (Carr-Zilber strain). This DNA preparation displays biological activity also in the culture of human embryo diploid cells (HEDC) which is manifested in: 1. discontinuous synthesis of avian oncovirus group-specific antigen; 2. enhancement of proliferative activity and morphological transformation of human cells; 3. continuous presence of virus-specific sequences as revealed by DNA/RNA hybridization. Producing complete oncornavirus by means of DNA isolated from Rous chick sarcoma in HEDC was unsuccessful. DNA preparation from gs negative chick embryo cells shows no infectious activity in HEDC culture.
D000956:Antigens, Viral; D001358:Avian Sarcoma Viruses / Q000276:immunology*; D002455:Cell Division; D002460:Cell Line; D002467:Cell Nucleus / Q000648:ultrastructure; D002471:Cell Transformation, Neoplastic; D004273:DNA, Neoplasm / Q000502:physiology*; D004279:DNA, Viral; D009693:Nucleic Acid Hybridization
D000956:Antigens, Viral; D004273:DNA, Neoplasm; D004279:DNA, Viral