Preliminary study of the use of dansyl chloride to determine cyclic-3',5'-AMP in tissues.
A highly sensitive method for the determination of cyclic-3',5'-AMP has been developed which involves the reaction of the substance with dansyl chloride and the subsequent separation of the dansyl-cyclic-3',5'-AMP derivative by thin-layer chromatography. Experiments with standard solutions of 3H-cyclic-3',5'-AMP have shown that there is a direct relationship between the amount of dansyl-3H-cyclic-3',5'-AMP recovered and that dansylated. The procedure is exceedingly sensitive, allowing milligram quantities of material to be analysed for its endogeneous cyclic-3',5'-AMP content. With the use of 14C-adenine as substrate, this method permits the separation of 14C-cyclic-3',5'-AMP formed from the substrate and other 14C-containing compounds, thus allowing the turn-over of cyclic-3'-5'-AMP to be studied. The usefullness of the method is demonstrated by analysing the turn-over and endogenous content of cyclic-3'-5'-AMP in rat nervous tissue.
D000818:Animals; D001923:Brain Chemistry; D002531:Cerebellum / Q000032:analysis; D002540:Cerebral Cortex / Q000032:analysis; D002855:Chromatography, Thin Layer / Q000379:methods; D000242:Cyclic AMP / Q000032:analysis*; D003619:Dansyl Compounds; D008297:Male; D008636:Mesencephalon / Q000032:analysis; D008832:Microchemistry; D009928:Organ Specificity; D051381:Rats
D003619:Dansyl Compounds; D000242:Cyclic AMP