Nicotinamide adenine dinucleotide-specific glutamate dehydrogenase of Neurospora. VIII. Isolation and sequences of peptides from a tryptic hydrolysate of the maleylated protein.
The NAD-specific glutamate dehydrogenase of Neurospora crassa was S-carboxymethylated with [14C]iodoacetate, maleylated, and hydrolyzed with trypsin. The isolation and sequences of the resulting peptides are described. These peptides gave information on the structure of the protein that was previously unknown and gave many overlaps of previously isolated segments of the protein.
D000595:Amino Acid Sequence; D005969:Glutamate Dehydrogenase; D008298:Maleates; D009243:NAD; D009491:Neurospora / Q000201:enzymology*; D009492:Neurospora crassa / Q000201:enzymology*; D010446:Peptide Fragments / Q000302:isolation & purification; D014357:Trypsin
D008298:Maleates; D010446:Peptide Fragments; D009243:NAD; D005969:Glutamate Dehydrogenase; D014357:Trypsin