Evidence for the replication of bovine leukemia virus in the B lymphocytes.
Bovine peripheral blood lymphocytes from a cow with persistent lymphocytosis were separated on nylon wool columns into nylon-adherent and nonadherent populations. Nylon-adherent cells were highly enriched for surface immunoglobulin (SIg) bearing B lymphocytes (95.5%) and nonadherent cells for SIg negative non-B cells, presumably T lymphocytes (96.3%). The B lymphocytes were found to be the major producers for bovine leukemia virus. A total of 39% of the B-enriched cells, surviving after 72 hours in culture, produced bovine leukemia virus as compared with 0.5% of the non-B cells.
D000818:Animals; D001402:B-Lymphocytes / Q000382:microbiology*; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology; D002469:Cell Separation; D005260:Female; D037102:Lectins / Q000494:pharmacology; D001909:Leukemia Virus, Bovine / Q000254:growth & development*; D008213:Lymphocyte Activation; D008228:Lymphoma, Non-Hodgkin / Q000382:microbiology / Q000662:veterinary; D012190:Retroviridae / Q000254:growth & development*; D014779:Virus Replication
D037102:Lectins