Subcellular distribution of cytochrome c oxidase, monoamine oxidase and lactate dehydrogenase in the developing chick telencephalon.
The distribution of cytochrome c oxidase monoamine oxidase and lactate dehydrogenase, together with protein, after isopycnic centrifugation of a crude mitochondrial fraction of chick telencephalon homogenate in a linear sucrose density gradient, was followed during late embryogenesis and postnatal maturation. Two main populations of subcellular organelles differentiate; they were characterized biochemically and analyzed by electron microscopy. One population, with a progressively defined mean buoyant density of 1.170 g/ml, exhibited a high relative activity of monoamine oxidase, with a low and relatively constant cytochrome c oxidase/monoamine oxidase activity ratio. This population was composed of free mitochondria and mitochondria enclosed in nerve endings, and possibly of mitochondria of perikaryal and glial origin. A second population, with a progressively well defined mean buoyant density of 1.182-1.186 g/ml, exhibited a high relative activity of cytochrome c oxidase, with a high and increasing cytochrome c oxidase/monoamine oxidase activity ratio. The biochemical and functional significance of these results were discussed.
D000818:Animals; D002458:Cell Fractionation; D002500:Centrifugation, Isopycnic; D002642:Chick Embryo; D003576:Electron Transport Complex IV / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D008928:Mitochondria / Q000201:enzymology; D008995:Monoamine Oxidase / Q000378:metabolism*; D013687:Telencephalon / Q000196:embryology / Q000201:enzymology* / Q000648:ultrastructure
D007770:L-Lactate Dehydrogenase; D008995:Monoamine Oxidase; D003576:Electron Transport Complex IV