Nerve growth factor increases activity of ornithine decarboxylase in superior cervical ganglia of young rats.
Nerve growth factor produces a rapid increase in the activity of ornithine decarboxylase (L-ornithine carboxy-lyase, EC 4.1.1.17) in superior cervical ganglia of young rats in vivo and in vitro. Maximum activity occurs 6-7 hr after the addition of nerve growth factor. The nerve growth factor-mediated increase in ornithine decarboxylase activity in vitro can be prevented by the addition of cycloheximide, actinomycin D, or antibody to nerve growth factor. A number of other agents were tested for their ability to increase ornithine decarboxylase were tested for their ability to increase ornithine decarboxylase activity in the ganglia; only nerve growth factor, and, to a slight extent, insulin were able to raise the activity of the enzyme. High concentrations of dibutyryl cyclic AMP (10 mM) were able to mimic the effect of nerve growth factor.
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002262:Carboxy-Lyases / Q000378:metabolism*; D003995:Dibutyryl Cyclic GMP / Q000494:pharmacology; D005727:Ganglia, Spinal / Q000201:enzymology*; D066298:In Vitro Techniques; D009414:Nerve Growth Factors / Q000494:pharmacology*; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology; D051381:Rats; D013268:Stimulation, Chemical; D013997:Time Factors
D009414:Nerve Growth Factors; D010726:Phosphodiesterase Inhibitors; D003995:Dibutyryl Cyclic GMP; D003994:Bucladesine; D002262:Carboxy-Lyases; D009955:Ornithine Decarboxylase