RNA polymerase associated with human rotaviruses in diarrhea stools.
RNA polymerase activity was detected in six stools which were partially purified by high-speed centrifugation from infants with rotavirus gastroenteritis, but was not detected in five stools which were negative for rotavirus by counterimmunoelectrophoresis and radioimmunoassay. The polymerase activity was associated with the 1.38-g/ml rotavirus band after purification in a CsCl gradient.
D002499:Centrifugation, Density Gradient; D012321:DNA-Directed RNA Polymerases / Q000302:isolation & purification*; D005243:Feces / Q000201:enzymology* / Q000382:microbiology; D006801:Humans; D007223:Infant; D012328:RNA Viruses / Q000201:enzymology*; D012401:Rotavirus / Q000201:enzymology*; D014777:Virus Diseases / Q000201:enzymology*
D012321:DNA-Directed RNA Polymerases