Preferential association of newly synthesized histones with replicating SV40 DNA.
The assembly of newly synthesized histones into nucleosomes during replication of SV40 minichromosomes in vivo was studied. Infected cells were labeled with 35S-methionine for a time shorter than that required to complete a round of viral DNA replication. Mature and replicating SV40 minichromosomes were extracted and separated by zonal sedimentation, and their histone content was analyzed by polyacrylamide gel electrophoresis (SDS and acidic urea). We show that the pulse-labeled histones associate preferentially with the replicating DNA.
D002460:Cell Line; D002843:Chromatin / Q000378:metabolism*; D004261:DNA Replication; D003852:Deoxyribonucleoproteins / Q000378:metabolism; D006657:Histones / Q000096:biosynthesis / Q000378:metabolism*; D013539:Simian virus 40 / Q000378:metabolism*; D014779:Virus Replication
D002843:Chromatin; D003852:Deoxyribonucleoproteins; D006657:Histones