Transformation of chondroblasts by Rous sarcoma virus and synthesis of the sulfated proteoglycan matrix.
The presence of the extracellular matrix synthesized by chondroblasts provides a barrier to virus penetration. Chondroblasts can be infected and transformed following treatment with proteolytic enzymes. Using a temperature-sensitive transformation mutant of Rous sarcoma virus and rearing the cells at permissive temperature, we demonstrate that transformed chondroblasts stop synthesizing their cell-unique sulfated proteoglycan. If such transformed chondroblasts are shifted to nonpermissive temperature, the cells reinitiate the synthesis of their cell-unique sulfated proteoglycan.
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000254:growth & development; D002356:Cartilage / Q000166:cytology*; D002455:Cell Division; D002471:Cell Transformation, Neoplastic; D002642:Chick Embryo; D046508:Culture Techniques; D009154:Mutation; D011509:Proteoglycans / Q000096:biosynthesis*; D013696:Temperature; D014779:Virus Replication
D011509:Proteoglycans