Dry weight, triglycerides content and glucose-6-phosphatase activity of hepatocytes separated by sedimentation.
1) Isolated rat-hepatocytes were subfractionated by isopycnic or velocity sedimentation, and dry mass, triglycerides content and Glucose-6-Phosphatase activity of different subpopulations were determined. 2) Distribution of the cell dry masses and dry mass per average cell were substantially similar in all the fractions separated by isopycnic sedimentation. Velocity sedimentation allowed a satisfactory separation of cells of different dry mass. 3) Triglycerides content and Glucose-6-Phosphatase activity of cells of the different fractions obtained by isopycnic sedimentation showed no statistically significant difference. Subpopulations fractionated by velocity sedimentation differed in both triglycerides content and Glucose-6-Phosphatase activity, which were substantially parallel to the cell dry mass.
D000818:Animals; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D005952:Glucose-6-Phosphatase / Q000032:analysis*; D006651:Histocytochemistry; D066298:In Vitro Techniques; D008099:Liver / Q000032:analysis* / Q000201:enzymology / Q000648:ultrastructure; D008297:Male; D051381:Rats; D014280:Triglycerides / Q000032:analysis*
D014280:Triglycerides; D005952:Glucose-6-Phosphatase