Comparison of guinea-pig serum lipoproteins after iodination by two different methods.
1. Guinea-pig low-density lipoproteins were isolated by ultracentrifugation and iodinated either by the IC1 method or by the chloramine-T procedure. 2. The efficiency of labelling by both methods was essentially the same. 3. When the two products were compared by ultracentrifugation, gel chromatography and immunodiffusion analysis, no significant difference in their properties was detected. 4. When they were compared by gradient-gel electrophoresis, aggregates were found in the product of the IC1 method, but not in the lipoprotein iodinated by the chloramine-T process. 5. Both products were metabolized by the guinea pig with essentially the same fractional catabolic rate. 6. The fractional catabolic rate of lipoprotein iodinated by the chloramine-T method was not significantly different from that of lipoprotein biologically labelled in the protein moiety with [75Se]selenomethionine. 7. It is concluded that the products of both methods of iodination are almost equally acceptable, provided that the optimum conditions for the chloramine-T reaction are carefully established.
D000818:Animals; D002700:Chloramines; D002712:Chlorides; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D006168:Guinea Pigs; D005779:Immunodiffusion; D007457:Iodine Radioisotopes; D007553:Isotope Labeling / Q000379:methods; D008077:Lipoproteins, LDL / Q000097:blood* / Q000302:isolation & purification; D014461:Ultracentrifugation
D002700:Chloramines; D002712:Chlorides; D007457:Iodine Radioisotopes; D008077:Lipoproteins, LDL