title: Studies on the respiratory system of Aspergillus oryzae. V. Some properties of the respiratory system of mitochondria from mycelia grown in the presence of chloramphenicol.
abstract: Presence of chloramphenicol in the growth medium for mycelia of Aspergillus oryzae was without effect on the oxidative activity, respiratory control, or P/O ratio of isolated mitochondria. The mitochondria oxidized Krebs cycle intermediates even in the presence of cyanide at the concentration markedly inhibiting the normal mitochondrial oxidation. However, the P/O ratio during the mitochondrial oxidation decreased by about 1.0 on addition of cyanide. The c-type cytochromes, shown to occur in large amounts than in normal mitochondria (Wakiyama and Ogura, 1972), were suggested to act as electron carriers in this cyanide-resistant oxidation. A novel pigment, demonstrated only in the mitochondria prepared from chloramphenicol-treated mycelia by a CO-difference spectrum, was presumed to be the terminal oxidase of the respiration in the presence of cyanide.
mesh_terms: D000968:Antimycin A / Q000494:pharmacology; D001230:Aspergillus / Q000378:metabolism*; D001236:Aspergillus oryzae / Q000378:metabolism* / Q000648:ultrastructure; D001665:Binding Sites; D002248:Carbon Monoxide; D002701:Chloramphenicol / Q000494:pharmacology*; D003470:Culture Media; D003486:Cyanides / Q000494:pharmacology; D003574:Cytochrome c Group / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D010085:Oxidative Phosphorylation / Q000187:drug effects; D010101:Oxygen Consumption / Q000187:drug effects*; D010860:Pigments, Biological
chemical_list: D003470:Culture Media; D003486:Cyanides; D003574:Cytochrome c Group; D010860:Pigments, Biological; D000968:Antimycin A; D002701:Chloramphenicol; D002248:Carbon Monoxide