High resolution preparative column chromatographic system for gangliosides using DEAE-Sephadex and a new porus silica, Iatrobeads.
A new high-resolution preparative column chromatographic system was developed for efficient and rapid isolation of ganglioside molecular species. The system involved a combination of ion-exchange and adsorption chromatographies using DEAE-Sephadex A-25 and the newly developed, totally porous silica spheres, Iatrobeads. Using this system the brain gangliosides, GM1, GD1a, GD1b and GT1 were obtained in high purity and in milligram amounts, in a relatively short time, by simple procedures. The presence of a number of unidentified molecular species of gangliosides, which are present only in small amounts, was also demonstrated.
D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002845:Chromatography / Q000379:methods; D002849:Chromatography, Gas; D002852:Chromatography, Ion Exchange / Q000379:methods; D002855:Chromatography, Thin Layer; D005732:Gangliosides / Q000302:isolation & purification*; D012822:Silicon Dioxide
D005732:Gangliosides; D012822:Silicon Dioxide