title: Chromatin-associated glycoproteins of normal rat liver and Novikoff hepatoma ascites cells.
abstract: Glycoproteins were demonstrated in the 0.6 M NaCl extract of chromatin of normal liver and Novikoff hepatoma cells by periodic acid-Schiff staining of sodium dodecyl sulfate-polyacrylamide gels. In chromatin extracts of Novikoff hepatoma cells, six major periodic acid-Schiff-positive bands coincident with Coomassie Blue-stained protein bands were found with molecular weights of 30,000, 54,000, 64,000, 75,000, 104,000, and 127,000. A corresponding extract from normal rat liver chromatin revealed the presence of four major periodic acid-Schiff-positive bands that migrated with protein bands at molecular weights of 16,000, 30,000, 54,000, and 75,000. The glycoproteins of these extracts contained either mannose or alpha-D-glucose, fucose, and N-acetylglucosamine as shown by the specificity of lectins in affinoelectrophoresis. One of the glycoproteins detected by affinoelectrophoresis was very basic.
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000032:analysis*; D002843:Chromatin / Q000032:analysis*; D004586:Electrophoresis; D006023:Glycoproteins / Q000032:analysis*; D037102:Lectins; D008099:Liver / Q000032:analysis*; D008113:Liver Neoplasms / Q000032:analysis*; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000032:analysis*; D009374:Neoplasms, Experimental / Q000032:analysis; D010503:Periodic Acid-Schiff Reaction; D051381:Rats
chemical_list: D002843:Chromatin; D006023:Glycoproteins; D037102:Lectins; D009363:Neoplasm Proteins