[Characterization of the viral-type nucleotide sequences detected in the RNA of human leukaemic cells by probes of murine and simian origins (author's transl)].
Molecular hybridization techniques were used for searching nucleic acid sequences homologous to murine and simian oncornaviral genomes in the RNA of various categories of human leukaemic cells. We report attempts to characterize the sequences that were detected in defined categories of leukemias. It is shown that: i) although there is some correlation between the two probes used in our study with regard to the positivity or the negativity of the tests for the same leukaemic cases, there are also some discrepancies since some of the cases are positive with one probe and negative with the other; ii) the common sequences of the two probes when isolated, were ineffective to detect any complementary sequences in leukemic cases which were scored as positive when the entire probes were used; iii) the complementary sequences detected in three positive cases of acute myelogenous leukemias by a recycled probe are additive and therefore most probably distributed along the viral genome.
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002478:Cells, Cultured; D004279:DNA, Viral; D006801:Humans; D066298:In Vitro Techniques; D007668:Kidney; D007938:Leukemia; D009052:Leukemia Virus, Murine; D008979:Moloney murine leukemia virus; D009711:Nucleotides; D012313:RNA / Q000032:analysis*; D051381:Rats; D012190:Retroviridae; D012842:Sarcoma Virus, Woolly Monkey; D009053:Sarcoma Viruses, Murine; D014776:Virus Cultivation
D004279:DNA, Viral; D009711:Nucleotides; D012313:RNA
Caractérisation des séquences nucléotidiques de type viral détectées dans le RNA des cellules leucémiques humaines par des sondes d'origine murine et simienne.