title: Adenylate kinase 2, a mitochondrial enzyme.
abstract: The subcellular compartmentalization of the isoenzymes of ATP:AMP phosphotransferase (adenylate kinase) was analyzed in HeLa cells, RAG cells, and RAG-human hybrids that express human AK-2. In HeLa cells and in the hybrids, human AK-2 was present in a mitochemical fraction prepared from cell extracts and in mitochondria purified by density gradient centrifugation. Human AK-1 was, as expected, distributed in the soluble cytoplasmic fraction of the cells. The rodent isozymes which are homologous to human AK-1 and AK-2 have been determined.
mesh_terms: D000263:Adenylate Kinase / Q000096:biosynthesis* / Q000302:isolation & purification; D000818:Animals; D006367:HeLa Cells / Q000201:enzymology; D006801:Humans; D006822:Hybrid Cells / Q000201:enzymology; D066298:In Vitro Techniques; D007527:Isoenzymes / Q000096:biosynthesis*; D051379:Mice; D008928:Mitochondria / Q000201:enzymology*; D010770:Phosphotransferases / Q000096:biosynthesis*
chemical_list: D007527:Isoenzymes; D010770:Phosphotransferases; D000263:Adenylate Kinase