Improved glycine-extracted complement-fixing antigen for human cytomegalovirus.
High-titered, sensitive, and stable complement-fixing antigens for human cytomegalovirus were consistently produced from human diploid fibroblast cells infected at a high multiplicity of virus, harvested after 7 days of incubation, and sonically treated immediately in 0.1 M glycine buffer, pH 9.5.
D000956:Antigens, Viral; D002460:Cell Line; D003168:Complement Fixation Tests; D003587:Cytomegalovirus / Q000276:immunology*; D006801:Humans; D008722:Methods
D000956:Antigens, Viral