title: HindIII and Sst I restriction sites mapped on rabbit poxvirus and vaccinia virus DNA.
abstract: The DNAs of two closely related orthopoxviruses, rabbit poxvirus (RPV) and vaccinia virus (VV), were mapped by overlapping-fragment analysis using restriction endonucleases HindIII and Sst I. The exact arrangement of these fragments was accomplished by total digestion of isolated partial restriction products and by end-fragment determination. RPV and VV DNAs showed identical restriction patterns in an internal region comprising approximately 60% of the genome. The size, by electrophoretical analysis of the RPV DNA, was 118 X 10(6) daltons, some 6 X 10(6) daltons less than VV DNA. The two opposite terminal restriction fragments of RPV DNA cross-hybridized to each other.
mesh_terms: D001483:Base Sequence; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004279:DNA, Viral / Q000032:analysis*; D004720:Endonucleases / Q000378:metabolism*; D005796:Genes; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D011212:Poxviridae / Q000032:analysis*; D014616:Vaccinia virus / Q000032:analysis*
chemical_list: D004279:DNA, Viral; D004720:Endonucleases; D004262:DNA Restriction Enzymes