Use of highly specific antibodies against 17alpha-OH-progesterone in a simplified nonchromatographic RIA and in the simultaneous determination of four sex hormones in human plasma.
A highly specific antiserum raised against the 3(O-carboxymethyl)oxime of 17alpha-hydroxyprogesterone (17-OHP) was produced in rabbit and used in the development of a specific radioimmunoassay (RIA) for 17-OHP which included a celite chromatography. Methods allowing the measurement of plasma 17-OHP levels either separately or in combination with that of several plasma androgens (testosterone, delta4-androstenedione and dehydroepiandrosterone) are described. Moreover, RIA meabsurement of plasma 17-OHP levels with or without chromatographic (celite column) purification gave comparable results (mean +/- SD) in 29 normal adult males (118 +/- 34 ng/100 ml) and 35 normal adult females (follicular phase: 46+/- 16 ng/100 ml); luteal phase: 241 +/- 71 ng/100 ml).
D000328:Adult; D000735:Androstenedione / Q000097:blood; D002845:Chromatography; D003429:Cross Reactions; D003687:Dehydroepiandrosterone / Q000097:blood; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D006908:Hydroxyprogesterones / Q000097:blood*; D008297:Male; D011863:Radioimmunoassay / Q000379:methods; D012016:Reference Values; D012822:Silicon Dioxide; D013739:Testosterone / Q000097:blood
D006908:Hydroxyprogesterones; D013739:Testosterone; D000735:Androstenedione; D003687:Dehydroepiandrosterone; D012822:Silicon Dioxide