[Proton relaxation times of water in normal and transformed cells infected with temperature sensitive virus mutants (author's transl)].
Nuclear magnetic resonance spin-lattice relaxation times (T1) of tissue water protons are elevated in samples from tumors when compared to the corresponding normal tissues. In order to exclude differences between the normal and the tumorous tissue samples other than transformation as far as possible the T1values of cells infected by temperature sensitive transforming mutants of Rous sarcoma virus were measured. In this model genetic homogeneity of the host cells as well as identity of the viruses in the normal and transformed state is assured. In the transformed cells the protons relaxed more slowly than in the cells of normal phenotype which were kept at an only slightly higher incubation temperature.
D000818:Animals; D001834:Body Water; D002471:Cell Transformation, Neoplastic; D004578:Electron Spin Resonance Spectroscopy; D005347:Fibroblasts; D006801:Humans; D066298:In Vitro Techniques; D009858:Oncogenic Viruses; D011522:Protons; D012513:Sarcoma, Experimental / Q000382:microbiology; D013696:Temperature
D011522:Protons
Spin-Gitter Relaxationszeiten der Wasserprotonen virusinfizierter Zellen vor und nach temperatursensitiver Transformation