Aminoacylation of encephalomyocarditis virus RNA.
RNA extracted from purified encephalomyocarditis (EMC) virus (EMC-RNA) can be aminoacylated with synthetase praparations from Escherichia coli, beef and rabbit liver. The extent of aminoacylation is between 0-024 and 0-080 moles per mole EMC-RNA and occurs only with serine. Either removal of possible low mol. wt. contaminants with 3 M-sodium acetate nor periodate oxidation of the virus RNA affects its aminoacylation capacity.
D000596:Amino Acids / Q000378:metabolism*; D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism; D004686:Encephalomyocarditis virus / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D012367:RNA, Viral / Q000378:metabolism*; D012694:Serine / Q000378:metabolism
D000596:Amino Acids; D012367:RNA, Viral; D012694:Serine; D000604:Amino Acyl-tRNA Synthetases