Size and location of poly (A) in encephalomyocarditis virus RNA.
Encephalomyocarditis (EMC) virus RNA contains a covalently bound sequence of polyriboadenylic acid (poly(A). This was determined by two-dimensional gel electrophoresis of complete T1 and pancreatic RNase digests of formamidesucrose gradient-purified RNA and subsequent analysis of the product by alkaline hydrolysis. The size of the EMC virus genomic poly(A) sequence was estimated by formamide-polyacrylamide gel electrophoresis of the RNase-resistant product, or by [3H-]poly(U) hybridization to freshly purified virion RNA, to be, on average, 40 nucleotides in length. The evidence obtained from [3H-]isoniazid labelling and other experiments would indicate that the poly(A) sequence is located at the 3'-terminus of EMC virus RNA.
D001483:Base Sequence; D004686:Encephalomyocarditis virus / Q000032:analysis*; D008970:Molecular Weight; D011061:Poly A / Q000032:analysis* / Q000302:isolation & purification; D012333:RNA, Messenger; D012367:RNA, Viral; D006163:Ribonuclease T1; D012260:Ribonucleases; D012265:Ribonucleotides / Q000032:analysis
D012333:RNA, Messenger; D012367:RNA, Viral; D012265:Ribonucleotides; D011061:Poly A; D012260:Ribonucleases; D006163:Ribonuclease T1