Neutralization of human serum lysozyme by sodium polyanethol sulfonate but not by sodium amylosulfate.
Sodium polyanethol sulfonate (SPS) at 500 microgram/ml, but not sodium amylosulfate (SAS) at 500 microgram/ml, precipitated egg white lysozyme (1 mg and 50 microgram of lysozyme per ml) as determined with the assay strain Micrococcus lysodeikticus ATCC 4698. Fresh and heat-inactivated (56 degrees C, 30 min) human serum (80%, vol/vol) killed M. lysodeikticus (10(4) bacteria per ml at zero time) within 1 to 2 h after exposure. Addition of 250 to 500 microgram of SPS per ml to fresh human serum protected M. lysodeikticus for 22 h as effectively as absorption of either fresh or heat-inactivated human serum with bentonite (10 mg/ml of serum, 10 min, 37 degrees C); the latter procedure is known to remove serum lysozyme. In contrast, SAS at 250 and 500 microgram/ml of serum retarded killing of the assay bacteria for periods of 4 h; after overnight (22 h) incubation, however, the number of M. lysodeikticus survivors had decreased significantly. The finding that SPS, but not SAS, at 250 to 500 microgram/ml effectively neutralized serum lysozyme-mediated killing of a lysozyme-sensitive assay strain may be of relevance with respect to laboratory processing of human blood culture specimens.
D000042:Absorption; D000687:Amylopectin / Q000494:pharmacology*; D001546:Bentonite / Q000494:pharmacology; D001557:Benzenesulfonates / Q000494:pharmacology*; D001770:Blood Bactericidal Activity / Q000187:drug effects; D011232:Chemical Precipitation; D004492:Edetic Acid / Q000494:pharmacology; D004529:Egg White; D004533:Egtazic Acid / Q000494:pharmacology; D006358:Hot Temperature; D006801:Humans; D008837:Micrococcus / Q000187:drug effects; D009113:Muramidase / Q000037:antagonists & inhibitors* / Q000097:blood / Q000494:pharmacology; D011074:Polyanetholesulfonate / Q000494:pharmacology*
D001557:Benzenesulfonates; D001546:Bentonite; D004533:Egtazic Acid; D011074:Polyanetholesulfonate; C001835:amylosulfate sodium; D000687:Amylopectin; D004492:Edetic Acid; D009113:Muramidase