Concentration of lipoproteins containing apolipoprotein B in human peripheral lymph.
The concentration of apolipoprotein B (apoB) in human serum and peripheral lymph was measured by quantitative immunoelectrophoresis with anti-serum to human low-density lipoprotein. In four normal and six hyperlipidaemic subjects, total lymph apob/ml was 5-10% of total serum apoB/ml in the same subject. These ratios were equivalent to lymph apob concentrations of 60-120 microgram/ml. When the assays were carried out under conditions in which unmasking of immunoreactive sites on lymph and serum apoB was assumed to be maximal (delipidation with Nonidet P40), the lymph/serum apoB concentration ratios in three normal subjects were similar to those obtained with untreated lymph and serum.
D000328:Adult; D001053:Apolipoproteins / Q000032:analysis* / Q000097:blood; D003902:Detergents; D005260:Female; D006801:Humans; D006949:Hyperlipidemias / Q000378:metabolism*; D008077:Lipoproteins, LDL / Q000032:analysis*; D008196:Lymph / Q000032:analysis*; D008297:Male; D008875:Middle Aged
D001053:Apolipoproteins; D003902:Detergents; D008077:Lipoproteins, LDL