Kinetics of glucose repression of yeast cytochrome c.
The kinetics of glucose repression of cytochrome c synthesis was measured by a radioimmune assay. When 5 or 10% glucose was added to a derepressed culture, the rate of cytochrome c synthesis was reduced to the repressed level with a half-life of 2 min. The addition of 1 or 0.5% glucose repressed the rate of cytochrome c synthesis to the same level as high glucose concentrations but with a longer half-life of 3 min. Glucose repression had no effect on the stability or function of the cytochrome c protein. Cellular levels of active cytochrome c mRNA during glucose repression were measured by translation of total cellular polyadenylic acid-containing RNA and immunoprecipitation cytochrome c from the translation products. The results of these measurements indicate that glucose represses the rate of cytochrome c synthesis through a reduction in the level of translatable cytochrome c mRNA.
D003574:Cytochrome c Group / Q000096:biosynthesis; D004794:Enzyme Repression; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D011863:Radioimmunoassay; D011887:Raffinose / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
D003574:Cytochrome c Group; D012333:RNA, Messenger; D005947:Glucose; D011887:Raffinose