An efficient method of antibody elution for the successive or simultaneous localization of two antigens by immunocytochemistry.
The method described in this paper is available for removal of antibodies retained by tissue antigen after immunohistochemical staining. Its application to the antehypophysis has allowed the successive or the simultaneous localization of two different hormones.
D000324:Adrenocorticotropic Hormone / Q000032:analysis*; D000818:Animals; D000906:Antibodies; D006168:Guinea Pigs; D007124:Immunoenzyme Techniques; D007158:Immunologic Techniques; D007986:Luteinizing Hormone / Q000032:analysis*; D010902:Pituitary Gland / Q000032:analysis*
D000906:Antibodies; D000324:Adrenocorticotropic Hormone; D007986:Luteinizing Hormone