Chilling of somatic cells unnecessary for Sendai virus-induced heterokaryon formation.
Incubation of cell suspensions or monolayers at 4 degrees C for 20 min after addition of beta-propiolactone-inactivated Sendai virus did not enhance heterokaryon formation as compared to parallel cell cultures incubated at 37 degrees C immediately after the addition of Sendai virus. These findings show that the routine chilling of somatic cells in fusion experiments can be omitted.
D002459:Cell Fusion; D002460:Cell Line; D003080:Cold Temperature; D006822:Hybrid Cells; D008722:Methods; D010222:Parainfluenza Virus 1, Human