Biochemical changes after hepatic injury by allyl alcohol and N-hydroxy-2-acetylaminofluorene.
Administration of hepatotoxic doses of allyl alcohol and N-hydroxy-2-acetylaminofluorene (N-OH-AAF) TO adult male rats produced periportal necrosis and functional derangement of the hepatic endoplasmic reticulum within 24 h. The rates of N-demethylation of ethylmorphine and p-hydroxylation of aniline were decreased 6 h following allyl alcohol administration, but cytochromes P-450 and b5 were unchanged. In contrast, administration of NOH-AAF decreased cytochromes P-450 and b5 and the rate of aniline p-hydroxylation, but did not change the rate of N-demethylation of ethylmorphine or the activities of cytochrome c reductase and glucose-6-phosphatase. No decrease was observed in the activity of the cytosol enzyme, DT diaphorase, following allyl alcohol treatment. The changes by these periportal hepatotoxins were compared with those produced both by central and midzonal hepatotoxins and with changes occurring in the liver after surgical partial hepatectomy.
D000082:Acetaminophen / Q000494:pharmacology; D000498:Allyl Compounds / Q000494:pharmacology*; D000815:Aniline Hydroxylase / Q000378:metabolism; D000818:Animals; D002251:Carbon Tetrachloride / Q000494:pharmacology; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D003580:Cytochromes / Q000378:metabolism; D003600:Cytosol / Q000201:enzymology; D004128:Dimethylnitrosamine / Q000494:pharmacology; D005037:Ethylmorphine-N-Demethylase / Q000378:metabolism; D005449:Fluorenes / Q000494:pharmacology*; D005665:Furosemide / Q000494:pharmacology; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D006881:Hydroxyacetylaminofluorene / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000293:injuries / Q000378:metabolism*; D008115:Liver Regeneration; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D009591:Nitrogenase / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D051381:Rats
D000498:Allyl Compounds; D003580:Cytochromes; D005449:Fluorenes; D011506:Proteins; D000082:Acetaminophen; D006881:Hydroxyacetylaminofluorene; D005665:Furosemide; D003577:Cytochrome P-450 Enzyme System; D002251:Carbon Tetrachloride; D000815:Aniline Hydroxylase; D009591:Nitrogenase; D005037:Ethylmorphine-N-Demethylase; D009251:NADPH-Ferrihemoprotein Reductase; D005952:Glucose-6-Phosphatase; D004128:Dimethylnitrosamine