[Obtaining iodinated luteinizing hormone while preserving its biological properties].
The author describes a method of obtaining biologically-active 125I-labeled luteinzing hormone in iodation with chloramine T. The hormone and chloramine T concentration ratio of 1:2, and the reaction time of 20 sec was used. LH-125I with the specific activity of 27--30 microCi/microgram was bound with the receptor strictly specifically.
D000818:Animals; D002700:Chloramines / Q000494:pharmacology; D006801:Humans; D007457:Iodine Radioisotopes; D007553:Isotope Labeling / Q000379:methods; D007986:Luteinizing Hormone / Q000138:chemical synthesis*; D008297:Male; D011485:Protein Binding / Q000187:drug effects; D051381:Rats; D011956:Receptors, Cell Surface / Q000187:drug effects; D013737:Testis / Q000187:drug effects
D002700:Chloramines; D007457:Iodine Radioisotopes; D011956:Receptors, Cell Surface; D007986:Luteinizing Hormone
Poluchenie ĭodirovannogo liuteiniziruiushchego gormona s sokhraneniem ego biologicheskikh svoĭstv.