Differential synthesis of blocked and unblocked 5'-termini in reovirus mRNA: effect of pyrophosphate and pyrophosphatase.
Viral mRNA synthesized in vitro by reovirusassociated enzymes contained 5'-terminal ppG, GpppG, or m7GpppGm. Selective synthesis of one type of 5'-end was obtained by adjusting the composition of the transcription reaction mixture based on a series of reactions proposed for formation of blocked, methylated 5'-terminal sequences of mRNA (capformation). The presence of inorganic pyrophosphate increased the proportion of ppG 5'-ends, while addition of pyrophosphatase yielded mRNA with 5'-terminal GpppG, which in the presence of a methyl donor was converted to m7GpppGm.
D011756:Diphosphates / Q000494:pharmacology; D006150:Guanine Nucleotides / Q000378:metabolism*; D012089:Mammalian orthoreovirus 3; D008745:Methylation; D011755:Pyrophosphatases / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis*; D012367:RNA, Viral / Q000096:biosynthesis*; D012436:S-Adenosylmethionine / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects
D011756:Diphosphates; D006150:Guanine Nucleotides; D012333:RNA, Messenger; D012367:RNA, Viral; D012436:S-Adenosylmethionine; D011755:Pyrophosphatases