Separation of hydroxyproline-containing protein from C1q (a subcomponent of complement) in serum.
Precipitation of the euglobulin fraction from serum separates C1q from another hydroxyproline-containing protein(s), which remains in the serum-minus-euglobulin fraction. The presence of C1q in the euglobulin fraction has been verified by radial immunodiffusion assay, by sensitivity to collagenase, by hydroxyproline content, and by electrofocusing. The presence of another hydroxyproline-containing protein(s) in the serum-minus-euglobulin fraction has been verified by the same criteria. The isoelectric pH range of C1q is 6.1-7.0; the isoelectric pH range of the other hydroxyproline-containing protein(s) is 4.2-5.5.
D001797:Blood Protein Electrophoresis; D001798:Blood Proteins / Q000302:isolation & purification*; D003172:Complement C1 / Q000302:isolation & purification*; D003165:Complement System Proteins / Q000302:isolation & purification*; D006801:Humans; D006909:Hydroxyproline / Q000097:blood*; D005779:Immunodiffusion; D007525:Isoelectric Focusing; D008722:Methods; D003012:Microbial Collagenase; D012712:Serum Globulins / Q000302:isolation & purification
D001798:Blood Proteins; D003172:Complement C1; D012712:Serum Globulins; D003165:Complement System Proteins; D003012:Microbial Collagenase; D006909:Hydroxyproline