title: Oxidation-reduction studies of the Mo-(2Fe-2S) protein from Desulfovibrio gigas.
abstract: Potentiometric titration followed by e.p.r. measurements were used to determine the midpoint reduction potentials of the redox centres of a molybdenum-containing iron-sulphur protein previously isolated from Desulfovibrio gigas, a sulphate-reducing bacterium (Moura, Xavier, Bruschi, Le Gall, Hall & Cammack (1976) Biochem. Biophys. Res. Commun. 728 782-789; Moura, Xavier, Bruschi, Le Gall & Cabral (1977) J. Less Common Metals 54, 555-562). The iron-sulphur centres could readily be distinguished into three types by means of g values, temperature effect, oxidation-reduction potential values and reduction rates. The type-I Fe-S centres are observed at 77 K. They show mid-point potential values of -260mV (Fe-S type IA) and -440 mV (Fe-S type IB). Centres of types IA and IB appear to have similar spectra at 77 K and 24 K. The Fe-S type-II centres are only observed below 65 K and have a midpoint potential of -28mV. Long equilibration times (30 min) with dye mediators under reducing conditions were necessary to observe the very slow equilibrating molybdenum signals. The potential values associated with this signal were estimated to be approx. -415 mV for Mo(VI)/Mo(V) and-530mV for Mo(V)/Mo(IV).
mesh_terms: D003901:Desulfovibrio; D004227:Dithionite; D004578:Electron Spin Resonance Spectroscopy; D005288:Ferredoxins; D008983:Molybdoferredoxin; D010084:Oxidation-Reduction; D013455:Sulfur
chemical_list: D005288:Ferredoxins; D008983:Molybdoferredoxin; D004227:Dithionite; D013455:Sulfur