Isolation and localization of RNA fractions able to transfer tumor-specific delayed hypersensitivity in vitro.
RNA fractions were prepared by sucrose density gradient centrifugation from hot-cold phenol, RNA-rich extracts of lymphoid tissues from strain 2 guinea pigs hyperimmunized to line 1 or line 10 tumors. Each RNA fraction was assessed for its ability to convert nonsensitized strain 2 peritoneal exudate cells to a state of specific sensitivity for line 1- or line 10-solubilized tumor antigens. An RNA fraction residing between the 4 S and 18 S peaks, designated as Fraction "B", transferred line 10 or line 1 sensitivity in 12 experiments. Twelve additional RNA extracts containing 2 subfractions prepared from RNA fraction B, designated as B1 and B2, also transferred line 1 or line 10 sensitivity in 14 experiments. Except for 3 experiments where the 4 S or 18 S material transferred tumor-specific sensitivity, RNA fractions corresponding to approximately 4 S, 18 S, 22 S, and 28 S were unable to transfer tumor-specific sensitivity to nonsensitized peritoneal exudate cells. Treatment of fraction B with RNase results in complete loss of ability to transfer immunobiological activity.
D000818:Animals; D000951:Antigens, Neoplasm; D001202:Ascitic Fluid / Q000166:cytology; D006528:Carcinoma, Hepatocellular / Q000276:immunology; D002464:Cell Migration Inhibition; D002499:Centrifugation, Density Gradient; D006168:Guinea Pigs; D006968:Hypersensitivity, Delayed / Q000276:immunology* / Q000378:metabolism; D007114:Immunization; D007116:Immunization, Passive; D008113:Liver Neoplasms / Q000276:immunology; D008198:Lymph Nodes / Q000276:immunology / Q000378:metabolism; D009374:Neoplasms, Experimental / Q000276:immunology*; D012313:RNA / Q000276:immunology* / Q000302:isolation & purification; D013154:Spleen / Q000276:immunology / Q000378:metabolism
D000951:Antigens, Neoplasm; D012313:RNA