Isolation from a transmissible lymphoid tumour (TLT) lymphoblastoid cell line of a herpesvirus similar to Marek's disease virus.
A chicken lymphoblastoid cell line (TLT)-6855 originally established from an avian oncornavirus-induced lymphoma (Siegfried and Olson, 1972) was studied for the presence and expression of Marek's disease virus (MDV) genome. By nucleic acid hybridization a significant amount of MDV DNA was found in this cell line. This virus DNA, however, was not expressed in either virus-specific intracellular or membrane antigens or the MD-associated tumour-specific surface antigen (MATSA). Moreover, MDV-specific antigens could not be activated in this cell line by treatment with 5-IdUrd. In several experiments, when chickens were inoculated with the cell line a herpesvirus was repeatedly isolated from the kidneys. This herpesvirus was antigenically similar to MDV but was low in oncogenicity for chickens.
D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D002645:Chickens; D004279:DNA, Viral / Q000032:analysis; D005347:Fibroblasts / Q000276:immunology / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D006564:Herpesviridae / Q000302:isolation & purification / Q000648:ultrastructure; D008381:Herpesvirus 2, Gallid / Q000276:immunology; D008854:Microscopy, Electron; D009693:Nucleic Acid Hybridization
D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D004279:DNA, Viral