5-Methyltetrahydrofolic acid, 5-formyltetrahydrofolic acid (folinic acid), and folic acid requirements of normal and Rous sarcoma virus-infected chicken fibroblasts.
Normal and Rous sarcoma virus-infected chicken fibroblasts proliferate maximally in a culture medium containing a physiological (10 ng/ml) concentration of 5-methyltetrahydrofolic acid or folinic acid (5-formyltetrahydrofolic acid), while their maximal proliferation requires a hyperphysiological (1000 ng/ml) concentration of folic acid. The normal and Rous-infected fibroblasts do not differ in their requirements for 5-methyltetrahydrofolate, folinic acid, or folic acid.
D000818:Animals; D001358:Avian Sarcoma Viruses; D002455:Cell Division / Q000187:drug effects; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D002645:Chickens; D003470:Culture Media; D002955:Leucovorin / Q000008:administration & dosage / Q000494:pharmacology*; D013763:Tetrahydrofolates / Q000008:administration & dosage / Q000494:pharmacology*
D003470:Culture Media; D013763:Tetrahydrofolates; D002955:Leucovorin