Procedures for radioimmunoassay of the mouse mammary tumor virus.
A procedure for radioimmunoassay of the major glycoprotein antigen derived from murine mammary tumor virus is described. The assay is sensitive to 0.05 ng of antigen and is highly reproducible. The antigen, gp55, has been found to be group specific and will detect viruses in 13 separate mouse strains, as wel  .l as from continuous cell lines. Factors affecting the assay have been examined.
D000818:Animals; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions / Q000187:drug effects; D000956:Antigens, Viral / Q000032:analysis*; D002460:Cell Line; D003429:Cross Reactions; D005990:Glycerol / Q000494:pharmacology; D006023:Glycoproteins / Q000032:analysis; D008324:Mammary Tumor Virus, Mouse / Q000276:immunology*; D051379:Mice; D008892:Milk / Q000382:microbiology; D011189:Potassium Chloride / Q000494:pharmacology; D011863:Radioimmunoassay; D014764:Viral Proteins / Q000032:analysis
D000956:Antigens, Viral; D006023:Glycoproteins; D014764:Viral Proteins; D011189:Potassium Chloride; D005990:Glycerol