Reexpression of HPRT activity following cell fusion with polyethylene glycol.
Polyethylene glycol-1000 (PEG-1000) induced fusion of HPRT (E.C. 2.4.2.8) deficient Chinese hamster cells with alpha-galactosidase A (E.C. 2.3.1.22) deficient cells from a patient with Fabry's disease yielded hybrids which contained both human and hamster HPRT, G6PD (E.C. 1.1.1.49), and APRT (E.C. 2.4.2.7) and Chinese hamster alpha-galactosidase B. Thus PEG-1000 mediated somatic cell fusion led to reexpression of Chinese hamster HPRT. It did not restore the expression of human alpha-galactosidase. Since PEG-1000 treatment of HPRT- Chinese hamster cells in the absence of human cells yielded no HPRT+ cells, it is concluded that the element responsible for the restoration of rodent HPRT was contributed by the human cells and not by the agent employed to promote fusion.
D000228:Adenine Phosphoribosyltransferase / Q000235:genetics; D000818:Animals; D002459:Cell Fusion / Q000187:drug effects*; D006224:Cricetinae; D005796:Genes; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics; D006801:Humans; D006822:Hybrid Cells / Q000201:enzymology*; D007041:Hypoxanthine Phosphoribosyltransferase / Q000096:biosynthesis / Q000235:genetics*; D007527:Isoenzymes / Q000235:genetics; D011092:Polyethylene Glycols / Q000494:pharmacology*; D000519:alpha-Galactosidase / Q000235:genetics
D007527:Isoenzymes; D011092:Polyethylene Glycols; D005954:Glucosephosphate Dehydrogenase; D000228:Adenine Phosphoribosyltransferase; D007041:Hypoxanthine Phosphoribosyltransferase; D000519:alpha-Galactosidase