[A method for the evaluation of nitroblue tetrazolium reduction in human platelets (author's transl)].
A long term incubation in NBT-solution leads not only to NBT-reduction by polymorphnuclear leukocytes and monocytes, but also in washed human platelets. The reaction is enhanced by ADP and to a smaller and very variable extent also by Thrombin and Ristocetin. It is inhibited by incubation in plasma environment as well by DBcAMP and PGE1. After a detailed demonstration of the applied method, some possible biochemical mechanisms of the NBT-reduction in human platelets are discussed.
D000244:Adenosine Diphosphate / Q000494:pharmacology; D001792:Blood Platelets / Q000378:metabolism*; D003994:Bucladesine / Q000494:pharmacology; D006801:Humans; D009000:Monocytes / Q000378:metabolism; D009504:Neutrophils / Q000378:metabolism; D009580:Nitroblue Tetrazolium / Q000378:metabolism*; D010084:Oxidation-Reduction; D011458:Prostaglandins E / Q000494:pharmacology; D012310:Ristocetin / Q000494:pharmacology; D013778:Tetrazolium Salts / Q000378:metabolism*; D013917:Thrombin / Q000494:pharmacology
D011458:Prostaglandins E; D013778:Tetrazolium Salts; D012310:Ristocetin; D009580:Nitroblue Tetrazolium; D000244:Adenosine Diphosphate; D003994:Bucladesine; D013917:Thrombin
Eine Methode zur Darstellung der Nitroblau--Tetrazoliumreduktion in menschlichen Thrombozyten.