Radioiodination studies of tumour cell-surface proteins after different disaggregation procedures.
The surface of single cells isolated from solid tumours by either a mechanical or an enzymatic method have been compared, using lactoperoxidase-catalyzed radioiodination of the tyrosine-containing proteins. Qualitatively, the patterns of surface labelling were similar, and duplicate experiments indicated that each method of isolation gave reproducible results. Analysis of incorporated label into 4 defined sections of the electrophoretic pattern illustrated quantitative differences. When the cells were isolated mechanically, the incorporation into low-mol.- wt. components was considerably reduced, whereas that into the high-mol.-wt. components was unaffected. Treatment of enzymatically isolated cells with trypsin also reduced incorporation into low-mol.-wt components.
D000818:Animals; D002462:Cell Membrane / Q000032:analysis; D002469:Cell Separation; D002470:Cell Survival; D006224:Cricetinae; D008228:Lymphoma, Non-Hodgkin / Q000032:analysis*; D008297:Male; D008565:Membrane Proteins / Q000032:analysis*; D003012:Microbial Collagenase; D009363:Neoplasm Proteins / Q000032:analysis*; D009374:Neoplasms, Experimental / Q000032:analysis
D008565:Membrane Proteins; D009363:Neoplasm Proteins; D003012:Microbial Collagenase