The effect of helper virus on Abelson virus-induced transformation of lymphoid cells.
Abelson murine leukemia virus (A-MuLV)-transformed fibroblast nonproducer cells were used to prepare A-MuLV stocks containing a number of different helper viruses. The oncogenicity of the A-MuLV stocks was tested by animal inoculation and their ability to transform normal mouse bone marrow cells was measured in vitro. All of the A-MuLV stocks transformed fibroblast cells efficiently. However, only A-MuLV stocks prepared with helper viruses that are highly oncogenic were efficient in vivo and in vitro in hematopoietic cell transformation. In addition, inefficient helpers did not establish a stable infection in lymphoid nonproducer cells. Thus, helper virus has a more central role in lymphoid cell transformation than in fibroblast cell transformation.
D000818:Animals; D001853:Bone Marrow / Q000382:microbiology; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D003673:Defective Viruses / Q000472:pathogenicity; D006378:Helper Viruses / Q000472:pathogenicity*; D009052:Leukemia Virus, Murine / Q000145:classification / Q000472:pathogenicity*; D007942:Leukemia, Experimental / Q000209:etiology; D051379:Mice; D014779:Virus Replication