In vitro growth characteristics and heterogeneity of mouse hepatitis virus type 3.
The in vitro virus yield of MHV3 reached 10(7) PFU/ml in mouse DBT cells infected with a virus suspension in HEPES-buffered medium containing DEAE-dextran. The virus titer was 10(6) PFU/ml in the presence of 10 micrograms actinomycin D/ml MHV3 growth in DBT cells gave three peaks of density (1.10--1.14 g/cm3, 1.18--1.20 g/cm3, and 1.25--1.31 g/cm3) in sucrose gradients. All these peaks retained infectivity.
D002460:Cell Line; D002499:Centrifugation, Density Gradient; D003609:Dactinomycin / Q000494:pharmacology; D006517:Murine hepatitis virus / Q000032:analysis / Q000254:growth & development*; D012313:RNA / Q000096:biosynthesis; D013696:Temperature; D014776:Virus Cultivation / Q000379:methods*; D014779:Virus Replication / Q000187:drug effects
D003609:Dactinomycin; D012313:RNA