A simple assay procedure for beta-D-mannanase.
A simple assay procedure for beta-D-mannanase enzyme has been developed which employs carob D-galacto-D-mannan dyed with Remazolbrilliant Blue. Additionally, the procedure is quantitative, relatively sensitive, and highly specific for beta-D-mannanase enzyme. It can be readily used for the determination of beta-D-mannanase activity in crude enzyme preparations and column-chromatography eluates.
D000880:Anthraquinones; D001190:Arylsulfonates; D004396:Coloring Agents; D006026:Glycoside Hydrolases / Q000032:analysis*; D008351:Mannans; D008722:Methods; D013379:Substrate Specificity
D000880:Anthraquinones; D001190:Arylsulfonates; D004396:Coloring Agents; D008351:Mannans; D006026:Glycoside Hydrolases