3H-noradrenaline outflow induced from isolated aventitia and intima-media of rabbit aorta by electrical field stimulation.
Electrical field stimulation of the isolated adventitial and intima-medial layers of rabbit aorta preloaded with 3H-noradrenaline elicited a 3H outflow. The initial 3H outflow from adventitia was independent of external Ca2+ concentration. Each of the subsequent 3H outflows was mainly Ca2+ sensitive. All stimulation-induced 3H outflows from media were Ca2+ insensitive. It is concluded that the isolated adventitia is a suitable preparation to outflow from aorta is of neuronal and extraneuronal origin.
D000818:Animals; D001011:Aorta / Q000294:innervation / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D004558:Electric Stimulation; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D009130:Muscle, Smooth / Q000294:innervation / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D009638:Norepinephrine / Q000378:metabolism*; D011817:Rabbits; D009435:Synaptic Transmission
D002118:Calcium; D009638:Norepinephrine