Kinase replacement by a dehydrogenase for Escherichia coli glycerol utilization.
A mutant of Escherichia coli that employs a glycerol:nicotinamide adenine dinucleotide 2-oxidoreductase (EC 1.1.1.6), instead of adenosine 5'-triphosphate:glycerol 3-phosphotransferase (EC 2.7.1.30), as the first enzyme for the dissimilation of glycerol was constructed. This mutant, like the wild-type strain, still cannot grow anaerobically on glycerol without an exogenous hydrogen acceptor.
D000332:Aerobiosis; D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism*; D005991:Glycerol Kinase / Q000378:metabolism*; D009154:Mutation; D009243:NAD / Q000378:metabolism; D010770:Phosphotransferases / Q000378:metabolism*
D009243:NAD; D000429:Alcohol Oxidoreductases; D010770:Phosphotransferases; D005991:Glycerol Kinase; D005990:Glycerol