Purification of infectious feline leukemia virus from large volumes of tissue culture fluids.
Feline leukemia virus from tissue culture fluids was concentrated 100 times by continuous-flow molecular filtration. Virus concentrates retained 100% of their original infectivity. Further purification was accomplished by a single sucrose density gradient centrifugation.
D002460:Cell Line; D002499:Centrifugation, Density Gradient; D005069:Evaluation Studies as Topic; D005256:Leukemia Virus, Feline / Q000302:isolation & purification*; D008851:Micropore Filters