Uridine--cytidine kinase from foetal and adult rat liver. Purification and study of some properties.
Partial purification of uridine--cytidine kinase (EC 2.7.1.48) from foetal rat liver by chromatography on DEAE-cellulose gives two active fractions. The first in order of elution was identified as a form specific for foetal liver. It was purified 300-fold. The second fraction was common to foetal, and adult rat liver and spleen and was purified 20-fold. The foetal fraction of the enzyme was found to be heat-sensitive and protected against inactivation by PO34- anions. The two isolated forms have different apparent Km for uridine, respectively 410 muM for the foetal form and 52 muM for the adult form.
D000818:Animals; D004355:Drug Stability; D005260:Female; D005333:Fetus; D006358:Hot Temperature; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008297:Male; D010770:Phosphotransferases / Q000378:metabolism*; D011247:Pregnancy; D051381:Rats; D013154:Spleen / Q000201:enzymology; D014541:Uridine Kinase / Q000302:isolation & purification / Q000378:metabolism*
D007527:Isoenzymes; D010770:Phosphotransferases; D014541:Uridine Kinase