Cholangiocarcinomas induced by feeding 3'-methyl-4-dimethylaminoazobenzene to rats. Histopathology and ultrastructure.
Thirty-three male Sprague-Dawley rats were fed a carcinogenic (0.064% 3'-methyl-4-dimethylaminoazobenzene, 3'-Me-DAB) ground meal normal diet. After 12 weeks the ground meal diet was replaced with a normal pellet diet, and the 30 surviving animals were divided into three equal groups. One group was sacrificed at the twelfth week and the other groups 4 and 8 weeks later. Control animals were also run. Based on previous studies which used "tumor-promoting" diets and 3'-Me-DAB, we expected a less than 100% incidence of predominantly hepatocellular carcinomas. However, we found mucin-producing cholangiocarcinomas in all 30 animals and, in addition, a small hepatocellular component in 3 of the animals. By electron microscopy the intestinal mucosal features of microvillous border cells, goblet cells, and endocrine-like cells were found. We suggest that the tumors produced as described here provide a good animal model of mucin-producing cholangiocarcinomas.
D002759:Adenoma, Bile Duct / Q000139:chemically induced* / Q000473:pathology; D000818:Animals; D001485:Basement Membrane / Q000648:ultrastructure; D002462:Cell Membrane / Q000648:ultrastructure; D002467:Cell Nucleus / Q000648:ultrastructure; D004032:Diet; D006056:Golgi Apparatus / Q000648:ultrastructure; D008099:Liver / Q000473:pathology; D008113:Liver Neoplasms / Q000139:chemically induced* / Q000473:pathology; D008297:Male; D008749:Methyldimethylaminoazobenzene; D008928:Mitochondria / Q000648:ultrastructure; D009374:Neoplasms, Experimental / Q000139:chemically induced*; D051381:Rats; D004124:p-Dimethylaminoazobenzene / Q000031:analogs & derivatives
D008749:Methyldimethylaminoazobenzene; D004124:p-Dimethylaminoazobenzene