[Use of the electron paramagnetic resonance method for the comparative evaluation of the effectiveness of the protective media used in the lyophilization of actimomycetes].
The accumulation of free radicals correlates with an increase in the percentage of killed lyophilized spores of actinomycetes. Therefore, the EPR method in combination with the method of "accelerated storage" at 37 degrees C was used to evaluate rapidly (during one month) the effectiveness and to select certain protective media for stabilization of actinomycetes cultures. The EPR method can be applied for comparative evaluation of only those media which did not contain originally free radicals.
D003451:Cryoprotective Agents / Q000494:pharmacology*; D004578:Electron Spin Resonance Spectroscopy / Q000379:methods*; D005609:Free Radicals; D005612:Freeze Drying; D013171:Spores, Bacterial / Q000187:drug effects; D013302:Streptomyces / Q000187:drug effects*; D013696:Temperature; D013997:Time Factors
D003451:Cryoprotective Agents; D005609:Free Radicals
Primenenie metoda élektronnogo paramagnitnogo rezonansa dlia sravnitel'noĭ otsenki éffektivnosti zashchitnykh sred, ispol'zuemykh pri liofilizatsii aktinomitsetov.