An enterotoxin produced by Clostridium perfringens type D. Purification by affinity chromatography.
Clostridium perfringens type D 9867 produced enterotoxin immunologically identical to that produced by types A and C which are responsible for C. perfringens food poisoning. Enterotoxin from type C 5386 and type D 9867 was produced in Duncan og Strong sporulation medium (DS medium). The supernatant fluid from DS-cultures was used for purification of the enterotoxin by affinity chromatography on a monospecific anti-enterotoxin-coupled CNBr activated-Sepharose 4B and activated CH-Sepharose 4B column. The enterotoxin purified by this one-step procedure proved to be of a purity comparable to that obtained by conventional methods, and possessed lethal activity in mice.
D002846:Chromatography, Affinity; D003016:Clostridium perfringens / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004768:Enterotoxins / Q000032:analysis / Q000096:biosynthesis / Q000302:isolation & purification*; D006386:Hemagglutination Tests; D006358:Hot Temperature; D005779:Immunodiffusion
D004768:Enterotoxins