A confirmation of the phase behavior of Escherichia coli cytoplasmic membrane lipids by X-ray diffraction.
The lipid fatty acid composition of the cytoplasmic membranes of Escherichia coli can be varied by growing an unsaturated fatty acid auxotroph in the presence of different fatty acid supplements. Electron spin resonance (ESR) studies of spin-label partitioning into the cytoplasmic membranes of different lipid fatty acid compositions as a function of temperature have been interpreted as indicating a broad order-to-disorder transition in the membrane lipids, the end points of the transition depending upon the fatty acid composition. We have utilized x-ray diffraction to confirm the ESR studies for three different fatty acid supplements (oleic, elaidic, and bromostearic). We found that the characteristic end-point temperatures detected by ESR were indeed the end-point temperatures of a broad order-to-disorder transition of the cytoplasmic membrane lipids. In addition, Patterson functions calculated from lamellar x-ray diffraction from partially oriented cytoplasmic membranes indicate a decrease in average membrane thickness upon fatty acid chain melting.
D002462:Cell Membrane / Q000648:ultrastructure; D004578:Electron Spin Resonance Spectroscopy; D004926:Escherichia coli / Q000648:ultrastructure*; D005227:Fatty Acids / Q000032:analysis; D008563:Membrane Lipids / Q000032:analysis; D008968:Molecular Conformation; D014961:X-Ray Diffraction
D005227:Fatty Acids; D008563:Membrane Lipids