Generation of new mouse sarcoma viruses in cell culture.
Endogenous nontumor-producing type C viruses from C3H mice were used to generate rapid, solid tumor-inducing variants in cell culture. The new mouse sarcoma viruses induce undifferentiated sarcomas with a short latency period upon inoculation into newborn NIH Swiss mice. Transforming viruses appear only transiently, at a time when the virus-infected cells show morphologic alterations; both before and after this time, transforming viruses cannot be detected. These results show that variants of endogenous type C virus which contain transforming genes (oncogenes) can arise during spread of the endogenous virus in fibroblast lines in vitro as well as in susceptible tissues in vivo.
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D005814:Genes, Viral; D051379:Mice; D012190:Retroviridae / Q000235:genetics / Q000472:pathogenicity*; D009053:Sarcoma Viruses, Murine / Q000235:genetics / Q000472:pathogenicity; D012513:Sarcoma, Experimental / Q000382:microbiology*