title: Stimulation of synthesis of the proteins of 30-S nuclear ribonucleoprotein particles in human amnion U cells by viral infection.
abstract: Early increase in RNA synthesis induced in human amnion U cells by infection with poliovirus is accompanied by an increased incorporation of amino acids into non-histone nuclear proteins with an approximate molecular weight of 40 000. These proteins are the main polypeptides of the 30-S nuclear ribonucleoprotein particles. After fractionation of nuclear proteins by extraction with solutions of different ionic strength, these polypeptides are present in the fraction of nuclear sap proteins soluble in 0.1 M Tris - HCl buffer, pH 7.6, and in the fraction of non-histone chromosomal proteins which are soluble in 0.35 M NaCl. The increase in synthesis of non-histone nuclear proteins with an approximate molecular weight of 40 000, observed in the infected cells, represents an increase in the synthesis of proteins concerned with post-transcriptional events and, therefore, is the result and not the cause of gene activation.
mesh_terms: D002250:Carbon Radioisotopes; D002460:Cell Line; D002868:Chromosomal Proteins, Non-Histone / Q000096:biosynthesis; D006801:Humans; D007553:Isotope Labeling; D008970:Molecular Weight; D009698:Nucleoproteins / Q000096:biosynthesis*; D017955:Poliovirus / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000096:biosynthesis*; D014316:Tritium
chemical_list: D002250:Carbon Radioisotopes; D002868:Chromosomal Proteins, Non-Histone; D009698:Nucleoproteins; D012261:Ribonucleoproteins; D014316:Tritium