Growth of cytomegalovirus at supra-optimal temperatures.
An isolate (Mira) of cytomegalovirus is shown to replicate in human embryonic lung fibroblasts at supra-optimal temperature (40 degrees C). The ability of the Mira isolate to grow at 40 degrees C decreased as a function of age of cells in which the virus was grown. The unusual morphology of the lesions in late passage cells infected and maintained at 40 degrees C is illustrated.
D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D003587:Cytomegalovirus / Q000254:growth & development*; D006801:Humans; D066298:In Vitro Techniques; D013696:Temperature; D014779:Virus Replication