Decrease of collagen biosynthesis ability of rat kidney fibroblasts transformed with SV--40 virus.
Rat kidney fibroblasts transformed with SV-40 produce "in vitro" a significantly lower amount of hydroxyproline-containing material which is collagenase sensitive as compared to normal cells. In contrast to normal fibroblast cultures, no collagenous material was found by histochemical methods in intercellular spaces of transformed cultures.
D000596:Amino Acids / Q000378:metabolism; D002460:Cell Line; D002472:Cell Transformation, Viral; D002850:Chromatography, Gel; D003094:Collagen / Q000096:biosynthesis*; D005347:Fibroblasts / Q000378:metabolism* / Q000648:ultrastructure; D006651:Histocytochemistry; D006909:Hydroxyproline / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D011392:Proline / Q000378:metabolism; D013539:Simian virus 40
D000596:Amino Acids; D003094:Collagen; D011392:Proline; D006909:Hydroxyproline