The effect of chronic diabetes, induced by streptozotocin, on the activities of some enzymes of glycerolipid synthesis in rat liver.
1. Rats were injected with a single dose of 35mg of streptozotocin/kg body wt. They exhibited a diabetes that was characterized by glycosuria, polyuria, polydipsia, hyperphagia, hyperglycaemia, increased concentrations of unesterified fatty acids, glycerol and triacylglycerols in the serum and an increased activity of glucose 6-phosphatase in the liver. 2. After 10 weeks the hepatic activities of the microsomal glycerol phosphate acyltransferase, phosphatidate phosphohydrolase, phosphatidate cytidylyltransferase, diacylglycerol acyltransferase, choline phosphotransferase, CDP-diacylglycerol--inositol phosphatidyltransferase and the soluble phosphatidate phosphohydrolase were measured. 3. The only significant changes were an increase in the activity of the soluble phosphatidate phosphohydrolase and a decrease in that of the CDP-diacylglycerol--inositol phosphatidyltransferase in the diabetic rats. 4. These results are discussed in relation to the control of glycerolipid synthesis.
D000217:Acyltransferases / Q000378:metabolism; D000818:Animals; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism; D002908:Chronic Disease; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology*; D002798:Diacylglycerol Cholinephosphotransferase / Q000378:metabolism; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D005989:Glycerides / Q000096:biosynthesis*; D005992:Glycerol-3-Phosphate O-Acyltransferase / Q000378:metabolism; D008055:Lipids / Q000096:biosynthesis*; D008297:Male; D008862:Microsomes, Liver / Q000201:enzymology*; D009713:Nucleotidyltransferases / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D010770:Phosphotransferases / Q000378:metabolism; D051381:Rats; D013311:Streptozocin
D005989:Glycerides; D008055:Lipids; D013311:Streptozocin; D000217:Acyltransferases; D005992:Glycerol-3-Phosphate O-Acyltransferase; D010770:Phosphotransferases; D009713:Nucleotidyltransferases; D002798:Diacylglycerol Cholinephosphotransferase; D002265:Carboxylic Ester Hydrolases; D010744:Phosphoric Monoester Hydrolases; D005952:Glucose-6-Phosphatase