Mutations causing charge alterations in regulatory subunits of the cAMP-dependent protein kinase of cultured S49 lymphoma cells.
Two-dimensional polyacrylamide gel electrophoresis is used to visualize the regulatory subunit of cAMP-dependent protein kinase from cultured S49 mouse lymphoma cells and to demonstrate its in vivo phosphorylation. Regulatory subunits from mutant cells with altered kinases exhibit at least two patterns of charge shifts consistent with substitutions of single amino acids. The direct demonstration of structural alteration of this protein provides strong evidence for structural gene mutation in this cultured cell system. While mutant and wild-type gene products co-exist in the mutant cells, there is apparently preferential expression and phosphorylation of mutant subunit in these heterozygotes.
D002460:Cell Line; D000242:Cyclic AMP; D004591:Electrophoresis, Polyacrylamide Gel; D005796:Genes; D009154:Mutation; D010710:Phosphates / Q000378:metabolism; D011494:Protein Kinases / Q000032:analysis / Q000096:biosynthesis* / Q000378:metabolism
D010710:Phosphates; D000242:Cyclic AMP; D011494:Protein Kinases