Sequence analysis of T1 ribonuclease fragments of 18S ribosomal RNA by 5'-terminal labeling, partial digestion, and homochromatography fingerprinting.
The method employed to determine the sequence of a T1 RNase fragment, A-A-A-A-A-U-A-A-C-A-A-U-A-C-A-Gp, from Novikoff rat hepatoma 18S ribosomal RNA is described. This method is applicable to any oligoribonucleotide produced by specific endonucleases that leave the newly cleaved 5'-end free for labeling with polynucleotide kinase and gamma-(32p)-ATP. The (32p)-labeled oligoribonucleotide is subjected to partial endonucleolytic digestion and fractionated by two-dimensional homochromatography fingerprinting. The nucleotide sequence is determined by following mobility shifts of the labeled and partially digested oligoribonucleotides in homochromatography fingerprinting.
D000818:Animals; D001483:Base Sequence; D006528:Carcinoma, Hepatocellular / Q000032:analysis; D008113:Liver Neoplasms / Q000032:analysis; D008722:Methods; D009374:Neoplasms, Experimental / Q000032:analysis; D009843:Oligoribonucleotides / Q000032:analysis; D012335:RNA, Ribosomal; D051381:Rats; D006163:Ribonuclease T1; D012260:Ribonucleases
D009843:Oligoribonucleotides; D012335:RNA, Ribosomal; D012260:Ribonucleases; D006163:Ribonuclease T1