Preparation of cell-free feline oncornavirus-associated cell membrane antigen.
Cell-free feline oncornavirus-associated cell membrane antigen (FOCMA) was prepared from a feline lymphoblastoid cell line of tumor origin (FL-74). Membrane fractions, separated on sucrose density gradients, and papain-solubilized products were found to contain FOCMA as determined by their capacity to inhibit reference cytotoxic cat antisera.
D000818:Animals; D000912:Antibodies, Neoplasm; D000914:Antibodies, Viral; D000951:Antigens, Neoplasm; D000956:Antigens, Viral / Q000032:analysis*; D002462:Cell Membrane / Q000276:immunology; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D003601:Cytotoxicity Tests, Immunologic; D005256:Leukemia Virus, Feline / Q000276:immunology; D007942:Leukemia, Experimental / Q000209:etiology / Q000276:immunology; D009858:Oncogenic Viruses / Q000276:immunology*; D010206:Papain; D005253:Sarcoma Viruses, Feline / Q000276:immunology
D000912:Antibodies, Neoplasm; D000914:Antibodies, Viral; D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D010206:Papain