title: Uptake of alpha-aminoisobutyric acid and phosphate by membrane vesicles derived from growing and quiescent fibroblasts.
abstract: Membrane vesicles derived principally from the plasma membrane and endoplasmic reticulum of mouse 3T3 cells transformed by Simian virus 40 take up alpha-aminoisobutyric acid (AIB) and phosphate (Pi). When NaCl is added simultaneously with AIB or Pi, uptake rises two- to three-times above the equilibrium to accumulate AIB or Pi over the control value, in the presence of a Na+ gradient, is almost lost in membrane vesicles derived from benzpyrene-transformed 3T3 cells (BP3T3) arrested in the G1 phase of the cell cycle by serum starvation. When added to the membranes with NaCl and the uptake substrate, a combination of fibroblast growth factor (FGF) and epidermal growth factor EGF restores the ability of the membranes to accumulate AIB and Pi over the control value.
mesh_terms: D000621:Aminoisobutyric Acids / Q000378:metabolism*; D000818:Animals; D001580:Benzopyrenes; D001693:Biological Transport, Active / Q000187:drug effects; D002455:Cell Division; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002471:Cell Transformation, Neoplastic; D004721:Endoplasmic Reticulum / Q000187:drug effects / Q000378:metabolism; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism*; D006133:Growth Substances / Q000494:pharmacology; D051379:Mice; D010710:Phosphates / Q000378:metabolism*; D013539:Simian virus 40; D012965:Sodium Chloride / Q000494:pharmacology
chemical_list: D000621:Aminoisobutyric Acids; D001580:Benzopyrenes; D006133:Growth Substances; D010710:Phosphates; D012965:Sodium Chloride; D004815:Epidermal Growth Factor