Production of antibody to tetanus toxoid by continuous human lymphoblastoid cell lines.
Peripheral lymphocytes from human volunteers boosted with tetanus toxoid were cultured after in vitro infection with Epstein-Barr virus. Forty-four continuous lymphoblastoid lines were established which continued to secrete human gamma globulin; seven of these secreted antibody to tetanus toxoid. Subcultures derived from limiting dilution experiments continued to secrete the antibody. Some of these antibody-secreting cells have been in continuous culture for more than 6 months.
D000917:Antibody Formation; D000918:Antibody Specificity; D002460:Cell Line; D002999:Clone Cells / Q000276:immunology; D004854:Herpesvirus 4, Human; D006652:Histological Techniques; D006801:Humans; D008214:Lymphocytes / Q000276:immunology*; D013743:Tetanus Antitoxin; D013745:Tetanus Toxoid
D013743:Tetanus Antitoxin; D013745:Tetanus Toxoid