[Isolation and purification of a preparation possessing leukocytosis-stimulating properties from pertussis bacteria].
A homogeneous protein LSF-2 preparation was extracted from the cultural fluid of Bordetella pertussis strains of the 1.0.3 serological type by means of precipitation with ammonium sulphate and electrofocussing; this preparation proved to produce a marked leukocytosis-stimulating and a weak toxic action of delayed type in experiments on animals. Intraperitoneal administration of 5 mug of the LSF-2 preparation caused a rise of leukocytosis in mice to 100,000 cells per 1 mm3, a delay in the gain in weight beginning from the 3rd day of the administration and a late death of the animals in 5% of cases. The LSF-2 preparation protected the mice in infection with a virulent pertussis strain No. 18323 in the amount of from 12 to 91%, depending on the immunizing dose; its ImD50 was equal to 2.0 -2.4 mug of protein. The results of investigations carried out permitted to assess the role of this substance in the formation of specific immunity in pertussis infection.
D000818:Animals; D000942:Antigens, Bacterial / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000302:isolation & purification; D001427:Bacterial Toxins / Q000302:isolation & purification*; D001835:Body Weight; D001886:Bordetella pertussis / Q000032:analysis*; D007964:Leukocytosis / Q000139:chemically induced*; D051379:Mice; D010567:Pertussis Vaccine / Q000302:isolation & purification*; D014611:Vaccination; D014917:Whooping Cough / Q000517:prevention & control
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D010567:Pertussis Vaccine
Vydelenie i ochistka preparata, obladaiushchego leĭkotsitozstimuliruiushchimi svoĭstvami, iz kokliushnykh bakteriĭ