Comparison of protein components of the plasma membrane fraction from rat liver and Morris hepatomas 5123D and 7777.
Isolated plasma membrane fractions from rat liver and Morris hepatoma 5123D and 7777 were labelled with radioiodine 125I by a chemical or enzymatic procedure and then were solubilized in 2 per cent solution of sodium dodecyl sulphate containing 1 per cent 2-mercaptoethanol. Solubilized proteins were separated into 20--22 zones staining with Coomassie Brilliant Blue R-250 after disc gel electrophoresis (7.5 per cent polyacrylamide gel). The high similarity of electrophoretic patterns of polypeptide components of all three preparations of cellular membranes was found in distinction to apparent differnces in the amount and disposition of substances stained with Schiff's reagent. Some tentative conclusions were drawn on the disposition of proteins within membrane structure studied by the method of labelling by chemical and enzymatic procedures (distinguishing between extrinsic and integral proteins).
D000818:Animals; D001192:Arylsulfatases / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000032:analysis* / Q000201:enzymology; D002462:Cell Membrane / Q000201:enzymology; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D007457:Iodine Radioisotopes; D008099:Liver / Q000032:analysis* / Q000201:enzymology; D008113:Liver Neoplasms / Q000032:analysis* / Q000201:enzymology; D008297:Male; D008565:Membrane Proteins / Q000032:analysis*; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000032:analysis*; D009374:Neoplasms, Experimental; D009708:Nucleotidases / Q000378:metabolism; D010455:Peptides / Q000032:analysis; D009195:Peroxidase; D051381:Rats; D013884:Thiosulfate Sulfurtransferase / Q000378:metabolism
D007457:Iodine Radioisotopes; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D010455:Peptides; D009195:Peroxidase; D013884:Thiosulfate Sulfurtransferase; D009708:Nucleotidases; D005952:Glucose-6-Phosphatase; D001192:Arylsulfatases