Cleavage of native type III collagen in the collagenase susceptible region by thermolysin.
Viscometric assays were used to demonstrate the activity of thermolysin (EC 3.4.24.4) on native type III collagen in solution. Analysis of the reaction products by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and electron microscopic visualisation of segment long spacing aggregates demonstrated localised cleavage of the collagen in the collagenase susceptible region.
D003094:Collagen / Q000378:metabolism*; D003012:Microbial Collagenase / Q000378:metabolism; D008970:Molecular Weight; D013379:Substrate Specificity; D013381:Subtilisins / Q000378:metabolism; D013820:Thermolysin / Q000378:metabolism*; D014357:Trypsin / Q000378:metabolism
D003094:Collagen; D013381:Subtilisins; D014357:Trypsin; D013820:Thermolysin; D003012:Microbial Collagenase