title: Adaptation of cells derived from human malignant tumours to growth in vitro.
abstract: Forty-five human malignant tumour specimens were cultivated in vitro in an attempt to determine the necessary conditions for tumour cell maintenance and to establish permanently-growing cell lines. Continuously-growing cultures were derived from five tumours, including carcinomas of the oesophagus and colon, a hepatoma, a mesothelioma and a retroperitoneal sarcoma. The carcinoma of the oesophagus and the hepatoma, which have adapted fully to in vitro conditions, can be regarded as established cell lines.
mesh_terms: D000222:Adaptation, Physiological; D006528:Carcinoma, Hepatocellular; D002455:Cell Division; D002460:Cell Line; D003110:Colonic Neoplasms; D004938:Esophageal Neoplasms; D005347:Fibroblasts / Q000254:growth & development; D006801:Humans; D008113:Liver Neoplasms; D008654:Mesothelioma; D009369:Neoplasms; D012509:Sarcoma; D014062:Tongue Neoplasms; D014357:Trypsin
chemical_list: D014357:Trypsin