title: Regulation of murine cytomegalovirus gene expression. I. Transcription during productive infection.
abstract: Murine cytomegalovirus RNA synthesis in productively infected mouse embryo cultures was measured by reassociation kinetics with iodinated viral DNA. The data were analyzed by a computer program and indicated the following: before DNA replication approximately 25% of the genome was transcribed into asymmetric transcripts, of which slightly fewer than half of the sequences were recovered from the cytoplasm. After viral DNA replication, approximately 38% of the genome was transcribed (5% as symmetric transcripts), and again less than half of the sequences appeared in the cytoplasm. Both early and late RNA comprised two abundance classes differing about 8- to 10-fold in concentration. Early RNA was a subset of late RNA. The RNE sequences synthesized in late-infected cells in the presence of cytosine arabinoside or cycloheximide were similar to early RNA. Thus, murine cytomegalovirus displays temporal, quantitative, and post-transcriptional controls over gene expression, but the pattern differs considerably from herpes simplex virus.
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology; D003561:Cytarabine / Q000494:pharmacology; D003587:Cytomegalovirus / Q000235:genetics* / Q000378:metabolism; D004261:DNA Replication; D004279:DNA, Viral / Q000096:biosynthesis; D051379:Mice; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000096:biosynthesis / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral; D003561:Cytarabine; D003513:Cycloheximide