title: Species-specific cellular DNA-binding proteins expressed in mouse cells transformed by chemical carcinogens.
abstract: Mouse cells transformed by DNA and RNA tumor viruses and by chemical carcinogens have been examined for the presence of specific DNA-binding proteins by DNA-cellulose chromatography. Using mouse DNA-cellulose we have obtained single-stranded DNA-binding proteins from two clones transformed by chemical carcinogens. Simian virus 40 transformants also have a DNA-binding protein [the tumor (T) antigen] that binds to mouse and human DNA with comparable affinity. Mouse sarcoma virus-transformed cells and two other chemically transformed clones showed no difference in DNA-binding protein pattern compared to the untransformed parental cell. The DNA-binding proteins isolated from the chemically transformed cell clones are between 25,000 and 30,000 daltons by sodium dodecyl sulfate/polyacrylamide gel electrophoresis. These cellular "T proteins" bind to the homologous mouse cellular DNA with a higher affinity than to heterologous human cellular DNA.
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000378:metabolism; D002273:Carcinogens; D002471:Cell Transformation, Neoplastic / Q000378:metabolism*; D002472:Cell Transformation, Viral; D004265:DNA Helicases / Q000378:metabolism*; D004267:DNA Viruses; D004277:DNA, Single-Stranded / Q000378:metabolism; D051379:Mice; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000378:metabolism*; D012328:RNA Viruses; D013045:Species Specificity
chemical_list: D000951:Antigens, Neoplasm; D002273:Carcinogens; D004277:DNA, Single-Stranded; D009363:Neoplasm Proteins; D004265:DNA Helicases