title: Inhibition of malate dehydrogenase by thyroxine and structurally related compounds.
abstract: The inhibition of pig heart mitochondrial malate dehydrogenase (L-malate: NAD+ oxidoreductase, EC 1.1.1.37) by the thyroxine and structurally related compounds was studied to resolve a longstanding question about the exact nature of the inhibition. Thyroxine, in freshly prepared solution, was found to be a "pure" competitive inhibitor relative to the nucleotide cofactor. Upon standing in diffuse daylight, solutions of thyroxine showed increased ability to inhibit the enzyme, presumably as a result of oxidation of enzyme sulfhydryl groups by free iodine that is released photochemically. This behavior probably accounts for earlier reports of irreversible inactivation by thyroxine. Comment is made on the implications of these findings to the mechanism of thyroid hormmone action.
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D008027:Light; D008291:Malate Dehydrogenase / Q000037:antagonists & inhibitors* / Q000528:radiation effects; D008929:Mitochondria, Heart / Q000201:enzymology; D009243:NAD / Q000494:pharmacology; D013552:Swine; D013974:Thyroxine / Q000494:pharmacology*; D014284:Triiodothyronine / Q000494:pharmacology
chemical_list: D014284:Triiodothyronine; D009243:NAD; D008291:Malate Dehydrogenase; D013974:Thyroxine