Prophage induction by alkylating ethyl methylaminosulfonate.
Treatment of the lysogenic strain M. lysodeikticus 53-40 (N5) with ethyl methylaminosulfonate results in a slight increase in infective center concentration of N5 phages which is only detectable once disturbing cells had been eliminated by lysozyme. After conversion of ethyl methylaminosulfonate with radioactive labelled guanosine at 37 degrees C, N7-ethyl-guanine could be ascertained. Therefore the biological activity of the drug may be due to an ethylation of DNA.
D000478:Alkylation; D001435:Bacteriophages / Q000187:drug effects; D005020:Ethyl Methanesulfonate / Q000031:analogs & derivatives / Q000494:pharmacology*; D006151:Guanosine; D008242:Lysogeny / Q000187:drug effects*; D008698:Mesylates / Q000494:pharmacology*; D008837:Micrococcus
D008698:Mesylates; D006151:Guanosine; D005020:Ethyl Methanesulfonate