[The effect of diisopropylfluorphosphate on the adenylate cyclase activity and the 3',5'-AMP content of myocardium and brain].
Adenylate cyclase [ATP-pyrophosphatelyase (cyclizing) EC 4.6.1.1.] from ventricular muscles and the cerebrum of rats can be inhibited by diisopropylphosphofluoridate (DFP) and paraxone in concentrations from 10(-7) - 10(-9)M, and by dimethoate in concentrations from 10(-5) - 10(-7)M. The cAMP content in the heart diminished after i.p. 1.44 mg/kg DFP by 59.9%, and in the cerebrum by 68.2%. The depletion of cAMP caused by double LD50 DFP in the rat heart can be influenced neither by atropine alone, nor in combination with TMB-4. The results are discussed with regard to a possible action mechanism.
D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D001921:Brain / Q000187:drug effects*; D000242:Cyclic AMP / Q000378:metabolism*; D004117:Dimethoate / Q000494:pharmacology; D005260:Female; D006321:Heart / Q000187:drug effects*; D007531:Isoflurophate / Q000494:pharmacology*; D008297:Male; D010261:Paraoxon / Q000494:pharmacology; D051381:Rats
D007531:Isoflurophate; D001285:Atropine; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D010261:Paraoxon; D004117:Dimethoate
Beeinflussung der Adenylatzyklaseaktivität und des 3',5'-AMP-Gehaltes in Herz und Grosshirn der Ratte durch Diisopropylfluorphosphat