A new extragenic suppressor of cya mutation. Mutant cyclic AMP receptor protein with an increased affinity for cyclic AMP.
A strain bearing an extragenic suppressor of cya mutation was isolated as a second-site revertant of an adenylate cyclase deficient strain. The mutant was unable to synthesize cAMP but showed normal fermentation profiles and growth properties on a variety of carbon sources. The site of reversion was mapped in, or near, the structural gene for the cAMP receptor protein. Structural alteration of the protein was directly demonstrated by the following biochemical observations: (i) A 10-fold decrease in the dissociation constant for cAMP, (ii) an acidic shift in the isoelectric point, and (iii) the altered binding properties to lambdah80dlac ps DNA.
D050260:Carbohydrate Metabolism; D000242:Cyclic AMP / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D005838:Genotype; D009154:Mutation; D010641:Phenotype; D011953:Receptors, Cyclic AMP / Q000378:metabolism*; D013045:Species Specificity
D011953:Receptors, Cyclic AMP; D000242:Cyclic AMP