The enzymic preparation of diphosphoinositides.
A procedure for the preparation of diphosphoinositides is described. Triphosphoinositides isolated from bovine brain are hydrolysed by the triphosphoinositide phosphatase (EC 3.1.3.36) from Crithidia fasciculata in the presence of MgC12 and cetyltrimethyl-ammonium bromide. The diphosphoinositides produced are not degraded further and can be recovered from the reaction mixture in greater than 80% yield. The product is chromatographically pure and has the same structure (1-phosphatidylinositol 4-phosphate) as naturally occurring diphosphoinositides.
D000818:Animals; D008722:Methods; D010716:Phosphatidylinositols / Q000096:biosynthesis* / Q000302:isolation & purification; D010744:Phosphoric Monoester Hydrolases; D014345:Trypanosoma / Q000201:enzymology
D010716:Phosphatidylinositols; D010744:Phosphoric Monoester Hydrolases