Immune response and latent infection after topical treatment of herpes simplex virus infection in hairless mice.
Treatment of herpes simplex virus (HSV)-infected hairless mice with a 2% phosphonoacetic acid (PAA) ointment prevented the appearance of virus-induced skin lesions and subsequent central nervous system (CNS) involvement. Treatment started 24 h after infection significantly reduced the intensity of the skin lesions and also prevented CNS involvement. After four to six applications of PAA ointment, a moderate skin erythemia developed, followed by scaling and complete healing 7 days after cessation of the treatment. Mice treated early after HSV infection had low or undetectable levels of virus-specific antibodies but were completely resistant to reinfection. Early treatment prevented the development of a latent ganglionic infection, but treatment initiated 24 h after infection could not prevent the establishment of the latent infection. PAA-treated and HSV-infected mice with nondetectable levels of antibodies did not develop, with a single exception, a latent ganglionic infection unpon reinfection. The cell-mediated immune response determined by levels of [14C]thymidine incorporation in Ficoll-Hypaque-purified spleen lymphocytes cultures was low in PAA-treated mice; it increased slightly after challenge infection but was strong in mice that proved to harbor a latent HSV infection in the ganglia.
D000085:Acetates / Q000627:therapeutic use*; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D000998:Antiviral Agents / Q000494:pharmacology / Q000627:therapeutic use*; D004353:Drug Evaluation, Preclinical; D005260:Female; D005727:Ganglia, Spinal / Q000382:microbiology; D006561:Herpes Simplex / Q000188:drug therapy / Q000276:immunology* / Q000382:microbiology / Q000517:prevention & control; D007109:Immunity; D007111:Immunity, Cellular; D051379:Mice; D008819:Mice, Nude; D009824:Ointments; D009943:Organophosphorus Compounds / Q000627:therapeutic use*; D018139:Simplexvirus / Q000187:drug effects / Q000302:isolation & purification
D000085:Acetates; D000914:Antibodies, Viral; D000998:Antiviral Agents; D009824:Ointments; D009943:Organophosphorus Compounds