[Cell transformation in vitro by transfer of isolated metaphase chromosomes].
Human and murine cells can be transformed in vitro following transfer of chromosomes (transfection) isolated from tumour (HeLa) or SV40-transformed (WI98VaD) human cells. An abortive transformation of Mouse cells is observed in soft-agar medium. An instability of the transformed phenotype is exhibited by the transfected human cells, following the isolation of colonies growing in soft-agar or low-serum medium. Nevertheless, two transformed cell lines (809 ch. VaD, Cl.5P and Cl.6P) could be established in culture.
D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D002875:Chromosomes; D004273:DNA, Neoplasm; D006367:HeLa Cells; D013539:Simian virus 40; D014162:Transfection
D000956:Antigens, Viral; D004273:DNA, Neoplasm
Transformation cellulaire in vitro par le transfert de chromosomes métaphasiques isolés.