title: Changes in enzyme activities during the artificially stimulated transition from follicular to luteal cell types in rat ovary.
abstract: This paper reports the time course of development of the intramitochondrial cholesterol side-chain-cleavage activity, cytoplasmic NADP+-dependent isocitric dehydrogenase, malic enzyme and glucose-6-phosphate dehydrogenase during ovarian maturation, using as a model the immature rat ovary stimulated to develop with pharmacological doses of gonadotrophin. These enzymic activities were correlated with increases in ovarian content of DNA, cellular content of adenosin 3':5'-monophosphate, and the levels of plasma progesterone. The plasma progesterone concentrations followed closely the development of the [4-14C]cholesterol side-chain-cleavage which was mimicked by the cytoplasmic isocitric dehydrogenase; both enzymes increased in activity 28 times during the 6 days of this study. There was no correlation between adenosine 3':5'-monophosphate levels and cholesterol side-chain cleavage or progesterone plasma concentrations.
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000378:metabolism; D003338:Corpus Luteum / Q000502:physiology*; D000242:Cyclic AMP / Q000378:metabolism; D004971:Estrus / Q000187:drug effects; D005260:Female; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D006064:Gonadotropins, Equine / Q000494:pharmacology*; D007521:Isocitrate Dehydrogenase / Q000378:metabolism; D008291:Malate Dehydrogenase / Q000378:metabolism; D008928:Mitochondria / Q000201:enzymology; D006080:Ovarian Follicle / Q000502:physiology*; D010053:Ovary / Q000187:drug effects / Q000201:enzymology*; D011247:Pregnancy; D011374:Progesterone / Q000378:metabolism; D051381:Rats
chemical_list: D006064:Gonadotropins, Equine; D011374:Progesterone; D000242:Cyclic AMP; D008291:Malate Dehydrogenase; D007521:Isocitrate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D002786:Cholesterol Side-Chain Cleavage Enzyme