[NAD-dependent formate dehydrogenase from methylotrophic bacteria. Isolation and properties].
NAD-Dependent formate dehydrogenase (FDH) has been isolated from methylotrophyc strain Bacterium sp 1 by (NH4)2SO4 fractionation of cell extract, ion-exchange chromatography and preparative isotachophoresis. Preparation of FDH is homogeneous in analytical polyacrylamide gel electrophoresis and under ultracentrifugation. Sedimentation coefficient of FDH is 4.9S. Mikhaelis constants are 1.1-10(-4) M for NAD and 1.5-10(-2) M for formate. In the absence of sulfhydril compounds FDH is unstable, but it is stable in the presence of mercaptoethanol or ditiotreitol.
D000445:Aldehyde Oxidoreductases / Q000302:isolation & purification / Q000378:metabolism*; D001419:Bacteria / Q000201:enzymology*; D005561:Formates; D007700:Kinetics; D008970:Molecular Weight; D009243:NAD
D005561:Formates; D009243:NAD; D000445:Aldehyde Oxidoreductases
NAD-zavisimaia formiatdegidrogenaza iz metilotrofnykh bakterii. Ochistka i osnovnye svoistva.