[The activity of polynucleotide phosphorylase in polyribosomes of regenerating liver of adult rats, liver of newborn rats and in some reinoculated tumours].
Specific activity and level of polynucleotide phosphorylase (PNPase) in polyribosomes of regenerating liver of adult rats, liver of newborn rats and in malignant tumours of rat (sarcoma M-1 and hepatoma 27) were studied. 24 hours after partial hepatectomy the specific activity and level of PNPase in regenerating liver decreased 3--4 times in the fraction of polyribosomes, bound to the endoplasmic reticulum membranes, and remained at a constantly low level in the fraction of free polyribosomes. The PNPase activity also showed a sharp decrease in the fraction of membrane-bound polyribosomes from newborn rats liver and could not be detected either in free or in bound polyribosomes from sarcoma M-1 or hepatoma 27. The PNPase activity in the fraction of bound polyribosomes increased with a decrease in the rate of liver growth (regenerating liver and newborn rats liver), and reached the level normal for adult animals. Possible mechanisms of regulation of the PNPase activity in animal tissue were studied. It was found that a 2-fold administration of cyclic 3,5'-AMP to intact animals (5 mg per 100 g of body weight) with an interval of 8 hours, corresponding to the interval between two peaks of the increase in cyclic 3,5'-AMP concentration following partial hepatectomy, diminished the PNPase specific activity in polyribosomes by 30%. A factor, presumably of protein origin, which induced a release of PNPase from polyribosomes of normal rat liver but did not affect the activity of the liberated enzyme, was detected in the cell sap of sarcoma M-1 and hepatoma 27.
D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D006528:Carcinoma, Hepatocellular / Q000201:enzymology*; D002458:Cell Fractionation; D000242:Cyclic AMP / Q000494:pharmacology; D003593:Cytoplasm / Q000201:enzymology; D004721:Endoplasmic Reticulum / Q000201:enzymology; D006498:Hepatectomy; D008099:Liver / Q000201:enzymology* / Q000502:physiology; D008113:Liver Neoplasms / Q000201:enzymology*; D008115:Liver Regeneration; D011117:Polyribonucleotide Nucleotidyltransferase / Q000378:metabolism*; D011132:Polyribosomes / Q000201:enzymology; D011485:Protein Binding; D051381:Rats; D012513:Sarcoma, Experimental / Q000201:enzymology*
D000242:Cyclic AMP; D011117:Polyribonucleotide Nucleotidyltransferase
Aktivnost' polinykleotidfosforilazy v polipibosomakh regeneriruiushcheĭ pecheni krys, novorozhdennykh krysiat i nekotorykh perevivaemykh opukholeĭ