Regulation of interferon production by dibutyryl cyclic GMP in serum-free human diploid cell cultures.
The mechanism for regulating interferon production was investigated in relation to accentuation of production in serum-free human diploid cells (strain WI-38) treated with N2,O2-dibutyryl guanosine 3',5'-cyclic monophosphate (db-cyclic GMP). Interferon production in serum-free WI-38 cell cultures in response to Newcastle disease virus (NDV) was greatly reduced. In these cells, there was decreased incorporation of 5-3H-uridine into the acid-insoluble fraction, but unimpaired incorporation of U-14C-L-leucine, as compared with serum-containing cultures. When serum-free cell cultures were treated with 0.2 mM-db-cyclic GMP, incorporation of both 5-3H-uridine and U-14C-L-leucine was increased and there was an 8-fold enhancement in the yield of interferon in response to NDV. Induction of db-cyclic GMP-treated cells by NDV in the presence of cycloheximide and actinomycin D suggests that db-cyclic GMP enhances transcription of the interferon gene, and thereby augments interferon production.
D001769:Blood; D002460:Cell Line; D003470:Culture Media; D006152:Cyclic GMP / Q000031:analogs & derivatives*; D003995:Dibutyryl Cyclic GMP / Q000494:pharmacology*; D004171:Diploidy; D006801:Humans; D007328:Insulin / Q000494:pharmacology; D007372:Interferons / Q000096:biosynthesis*; D009522:Newcastle disease virus / Q000254:growth & development; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis
D003470:Culture Media; D007328:Insulin; D003995:Dibutyryl Cyclic GMP; D012313:RNA; D007372:Interferons; D006152:Cyclic GMP