[Effect of mutations in regulatory genes for alkaline phosphatase on the phosphohydrolase spectrum of E. coli periplasm].
The isoform spectra of alkaline and acid phosphatase, pyrophosphataes, and ATPase in periplasm of E. coli were studied using electrophoresis in polyacrylamide gel with subsequent development of zimograms directly in the gel. Wild strains and mutants on 4 regulatory genes of alkaline phosphatase were analyzed. Mutations in regulatory genes were shown to influence the amount of each of the 3 isoforms of alkaline phosphatase and also the spectra of other phosphohydrolases.
D000135:Acid Phosphatase / Q000235:genetics; D000251:Adenosine Triphosphatases / Q000235:genetics; D000469:Alkaline Phosphatase / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005809:Genes, Regulator; D009154:Mutation; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics*; D011755:Pyrophosphatases / Q000235:genetics
D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D010744:Phosphoric Monoester Hydrolases; D000251:Adenosine Triphosphatases; D011755:Pyrophosphatases
Vliianie mutatsiĭ v reguliatornykh genakh shchelochoĭ fosfatazy na fosfogidrolaznyĭ spektr periplazmy E. coli.