Ionophore A23187 and dibutyryl cyclic AMP effects on cell shape and morphology of B-16 melanoma.
Melanoma cells treated with dibutyryl cyclic AMP (db cAMP) for 24 h resulted in dendritic cells possessing parallel assembled microtubules. A23187 treatments resulted in a biphasic response: Long term effects of the ionophore were characterized by small epitheloid cells while the immediate response produced elongated cells with parallel arranged 10 nm microgilaments, characteristic of dispersive melanocytes.
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003994:Bucladesine / Q000494:pharmacology*; D000001:Calcimycin / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000648:ultrastructure; D008545:Melanoma / Q000648:ultrastructure*; D051379:Mice; D008810:Mice, Inbred C57BL; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D008870:Microtubules / Q000648:ultrastructure; D009374:Neoplasms, Experimental
D000900:Anti-Bacterial Agents; D000001:Calcimycin; D003994:Bucladesine; D002118:Calcium