[Concentration of different forms of RNA in the brain and ribonuclease activity of the nuclear, ribosomal and supernatant fractions of brain tissue following the action of cyclic adenosine-3',5'-monophosphate and S-adenosyl-L-methionine].
Quantitative changes of various forms of ribonucleic acids [nuclear (n-RNA), ribosomal (r-RNA), transport (t-RNA)] as well as ribonuclease activity have been studied in rat brain, in its nuclear, ribosomal and supernatant fractions following 3,5-cyclic AMP (c-AMP) and S-adenosyl-L-methionine. These substances were shown to raise the levels of r-RNA in brain and reduce the amount of n-RNA of GC type. c-AMP was also found to reduce the n-RNA of AU type and t-RNA in brain, while S-adenosyl-L-methionine does not affect n-RNA of AU type and raises considerably t-RNA. S-adenosyl-L-methionine has been found to raise the levels of all kinds of RNA while c-AMP has no effect. Ribonuclease activity of nuclear, ribosomal and supernatant fractions (105,000 g) of brain has been found to be enhanced by c-AMP while S-adenosyl-L-methionine raises ribonuclease activity of ribosomal fraction only at pH 7.9. The data obtained indicate that c-AMP and S-adenosyl-L-methionine are of importance in the mechanisms regulating the level of nucleic acids and activity of enzymes involved in their metabolism.
D000818:Animals; D001921:Brain / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure; D002467:Cell Nucleus / Q000201:enzymology; D000242:Cyclic AMP / Q000494:pharmacology*; D008297:Male; D012313:RNA / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000378:metabolism; D012343:RNA, Transfer / Q000378:metabolism; D051381:Rats; D012260:Ribonucleases / Q000378:metabolism*; D012270:Ribosomes / Q000201:enzymology; D012436:S-Adenosylmethionine / Q000494:pharmacology*
D012335:RNA, Ribosomal; D012313:RNA; D012436:S-Adenosylmethionine; D012343:RNA, Transfer; D000242:Cyclic AMP; D012260:Ribonucleases
Soderzhanie razlichnykh form RNK v mozgu i aktivnost' ribonukleazy iadernoĭ, ribosomnoĭ i nadosadochnoĭ fraktsii mozgovoĭ tkani pri deĭstvii tsiklicheskogo adenozin-3',5'-monofosfata i S-adenozil-L-metionina