title: Effect of pasteurization and heat treatment on bovine leukemia virus.
abstract: Four sheep inoculated with mixtures of bovine leukemia virus (BLV) and milk that was treated by a simulated high-temperature short-time pasteurization procedure did not become infected with BLV or develop tumor. Four sheep inoculated with unpasteurized BLV-mil mixtures became infected with BLV and 3 died with tumor at 15, 21, and 27 months, respectively, after inoculation. Fluid from a BLV-infected cell culture was heated to 56, 60, 65, and 73 C for 1/2 minute and 1 minute (in separate trials) and transferred to noninfected cells. Culture fluid heated to 60 C and higher did not infect the cells.
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D003470:Culture Media; D005260:Female; D006358:Hot Temperature; D001909:Leukemia Virus, Bovine / Q000254:growth & development; D008228:Lymphoma, Non-Hodgkin / Q000209:etiology / Q000662:veterinary; D008297:Male; D008892:Milk / Q000382:microbiology*; D012190:Retroviridae; D012756:Sheep; D012757:Sheep Diseases / Q000209:etiology; D013242:Sterilization
chemical_list: D003470:Culture Media