Angiotensin converting enzyme: induction by steroids in rabbit alveolar macrophages in culture.
Dexamethasone and prednisone in physiologic range increased angiotensin converting enzyme 7- to 16-fold in comparison to control in 3 days at maximal stimulation (4 nM steroid) in rabbit alveolar macrophages in culture. The increase was inhibited by actinomycin D (0.1 microng/ml) and 1 micronM cycloheximide, suggesting that de novo transcription and enzyme synthesis are responsible for the increased enzyme activity. This result is evidence for a regulatory mechanism for angiotensin converting enzyme, which is important in blood pressure control.
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D003078:Colchicine / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology*; D004790:Enzyme Induction / Q000187:drug effects; D008264:Macrophages / Q000201:enzymology*; D007703:Peptidyl-Dipeptidase A / Q000096:biosynthesis / Q000378:metabolism*; D011241:Prednisone / Q000494:pharmacology*; D011650:Pulmonary Alveoli / Q000166:cytology; D011817:Rabbits
D003609:Dactinomycin; D003907:Dexamethasone; D007703:Peptidyl-Dipeptidase A; D003078:Colchicine; D011241:Prednisone