Estimation of transmembrane potentials from phase equilibria of hydrophobic paramagnetic ions.
Positively charged hydrophobic spin labels have been synthesized which respond to transmembrane potentials in sonicated liposomes. Electron paramagnetic resonance spectroscopy is used to show that the distribution of these probes between aqueous and membrane phases changes as a function of transmembrane potential. When liposomes are made more inside-negative, the fraction of membrane associated probe increases while the fraction of probe in the aqueous phase decreases. The results are in quantitative agreement with a simple equilibrium thermodynamic theory which allows estimation of absolute transmembrane potentials in phospholipid vesicles.
D004578:Electron Spin Resonance Spectroscopy; D008433:Mathematics; D008564:Membrane Potentials; D008567:Membranes, Artificial; D008954:Models, Biological; D010713:Phosphatidylcholines; D013816:Thermodynamics
D008567:Membranes, Artificial; D010713:Phosphatidylcholines