Interactions between 125-I-labelled RNA from RNA tumor viruses and RNA of other sources.
Fragmented 125I-labelled RNA from RNA tumor viruses was hybridized to unlabelled RNA from cells, viruses, and homoribopolymers. The viral RNA interacted with all RNA tested, except for certain homoribopolymers. Complex formation with unlabelled RNA was verified by nuclease resistance, buoyant density measurements, and thermal stability in solutions of different ionic strength. The RNAase-resistant complex involved 20-30% of the sequences in the 125I-labelled viral RNA and formed preferentially with nuclear RNA of cells. 125I-labelled hemoglobin mRNA, 125I-labelled immunoglobulin light chain (lambda2) mRNA, or 125I-labelled viral RNA from encephalomyocarditis virus (EMC) dit not from RNAase-resistant complexes with unlabelled cellular RNA.
D000818:Animals; D001854:Bone Marrow Cells; D002467:Cell Nucleus; D002645:Chickens; D003593:Cytoplasm; D004686:Encephalomyocarditis virus; D006454:Hemoglobins; D006801:Humans; D007146:Immunoglobulin lambda-Chains; D007962:Leukocytes; D051379:Mice; D009693:Nucleic Acid Hybridization; D009858:Oncogenic Viruses; D011131:Polyribonucleotides; D012313:RNA; D012333:RNA, Messenger; D012343:RNA, Transfer; D012367:RNA, Viral; D011817:Rabbits; D011925:Rauscher Virus; D012260:Ribonucleases
D006454:Hemoglobins; D007146:Immunoglobulin lambda-Chains; D011131:Polyribonucleotides; D012333:RNA, Messenger; D012367:RNA, Viral; D012313:RNA; D012343:RNA, Transfer; D012260:Ribonucleases