[Method of preparing a radioactive collagen substrate from chick embryo tendons for determining the activity of collagenases of animal origin].
A method, described for obtaining of 14C-collagen from chicken embryo tendons, permitted to isolate the highly purified native substrate with high specific radioactivity applied to estimation of animal collagenases activity. The method is relatively simple, reproducible, does not require large amount of labelled amino acids; it shortens the course of protein purification.
D000818:Animals; D002250:Carbon Radioisotopes; D002642:Chick Embryo; D003094:Collagen / Q000302:isolation & purification*; D007553:Isotope Labeling / Q000379:methods*; D003012:Microbial Collagenase / Q000378:metabolism*; D013710:Tendons / Q000032:analysis / Q000196:embryology
D002250:Carbon Radioisotopes; D003094:Collagen; D003012:Microbial Collagenase
Poluchenie radioaktivnogo kollagenovogo substrata iz sukhozhiliĭ kurinykh émbrionov dlia opredeleniia aktivnosti kollagenaz zhivotnogo proiskhozhdeniia.