Studies of H-2 restriction in cell-mediated cytotoxicity and transplantation immunity to Leukemia-associated antigens.
H-2 dependency of T cell-mediated cytotoxicity and transplantation immunity to leukemia-associated antigens has been investigated. Through the use of a 20-hr 125IUdR release assay, it was found that the induction of T cell-mediated cytotoxicity against Friend virus-induced leukemias of different H-2 haplotype orgins could be produced by immunization with both syngeneic and allogeneic tumor cells; the effector cells that were generated by syngeneic immunization could also provide effective killing of allogeneic tumor cells, although the killing of allogeneic targets might require a longer incubation time (20 to 40 hr). Furthermore, in vivo transplantation immunity against Friend virus-induced leukemias also was induced by immunization with both syngeneic and allogeneic tumors and syngeneic immunization could induce specific protection against the challenge with a-logeneic tumor in x-irradiated hosts. These findings clearly indicate that, both at the sensitizing phase and effector phase of the immune response, there is no strict H-2 dependency for T cell-mediated cytotoxicity or in in vivo transplantation imunity to leukemia-associated antigens.
D000818:Animals; D000951:Antigens, Neoplasm; D000961:Antilymphocyte Serum; D002857:Chromium / Q000378:metabolism; D003601:Cytotoxicity Tests, Immunologic; D005260:Female; D006648:Histocompatibility; D007065:Idoxuridine / Q000378:metabolism; D007111:Immunity, Cellular; D007519:Isoantigens; D009052:Leukemia Virus, Murine; D007942:Leukemia, Experimental / Q000209:etiology / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D013045:Species Specificity; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D014181:Transplantation Immunology; D014185:Transplantation, Isogeneic
D000951:Antigens, Neoplasm; D000961:Antilymphocyte Serum; D007519:Isoantigens; D002857:Chromium; D007065:Idoxuridine