title: [Standardization of the control technics of Clostridium perfringens vaccine. Use of a reference vaccine].
abstract: Control of Cl perfringens toxins. It is possible to employ a standardized technique for the control of the toxins of Cl. perfringens. Titres of LD50 per ml for the mouse and L+/50 per ml may be expressed with a confidence limit of 1.5. The assay of toxins by L+/50 (relating to their antigenic value) permits the establishment of standards by which it is possible to obtain a satisfactory vaccine with a high degree of certainty. 10 L+/50 per dose for Cl. perfringens A 20 L+/50 per dose for Cl. perfringens B 180 L+/50 per dose for Cl. perfringens C 50 L+/50 per dose for Cl. perfringens D Control of vaccine values. Technique adopted : Determination of antibodies in the rabbit following vaccination. The confidence limit of the antitoxin titre is similarly equal to 1.5 when P = 0.05. It would not appear to be necessary to establish minimum levels of antibodies to confer protection; it seems nevertheless that the results are influenced by considerable variations attributable to the animals used for the most part and that a reference vaccine is required. Freeze-dried vaccine. There is a natural antigenic activity in the rabbit which retains a considerable part of its antigenic properties after maintenance for one week at +37 degrees C. In our view this would be suitable for a reference vaccine.
mesh_terms: D000818:Animals; D000992:Antitoxins / Q000032:analysis; D001427:Bacterial Toxins / Q000592:standards; D001428:Bacterial Vaccines / Q000592:standards*; D003016:Clostridium perfringens / Q000254:growth & development / Q000276:immunology*; D008722:Methods; D051379:Mice; D011817:Rabbits; D014118:Toxins, Biological / Q000302:isolation & purification
chemical_list: D000992:Antitoxins; D001427:Bacterial Toxins; D001428:Bacterial Vaccines; D014118:Toxins, Biological
vernacular_title: Standardisation des techniques de contrôle des vaccins a Clostridium perfringens. Utilisation d'un vaccin de référence