The use of a complementary DNA probe to detect accumulation of mengo RNA in infected cells pretreated with interferon.
Complementary DNA (cDNA) from Mengo virus RNA has been synthesized and used as a probe to measure the synthesis and accumulation of viral RNA in Mengo infected L cell cultures, treated or untreated with interferon. Under experimental conditions used (200 units interferon/ml and 50 virus plaque-forming units/cell) results show that there is some synthesis of Mengo virus RNA in cells treated with interferon. One hour after infection, treated cells contain three times less viral RNA than untreated cells; five hours after infection, this difference has increased to ten fold. As in the control, no fragmented Mengo virus RNA molecules were found in interferon treated cells. The smaller recovery of infectious particles from interferon treated cells as compared to RNA accumulation suggests that not only RNA accumulation is inhibited but also a step posterior in viral maturation.
D003593:Cytoplasm / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism*; D005914:Globins; D007372:Interferons / Q000494:pharmacology*; D007700:Kinetics; D007739:L Cells / Q000187:drug effects / Q000378:metabolism; D008574:Mengovirus; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger; D012367:RNA, Viral / Q000096:biosynthesis / Q000378:metabolism*; D013997:Time Factors
D004279:DNA, Viral; D012333:RNA, Messenger; D012367:RNA, Viral; D005914:Globins; D007372:Interferons