Maintenance of viability and comparison of identification methods for influenza and other respiratory viruses of humans.
A comparison of Hanks balanced salt solution, veal infusion broth (VIB), and charcoal viral transport medium for maintaining viability of type A influenza virus indicated approximately equal survival of virus on all three media at -70 and 4 degrees C, whereas at 25 degrees C virus survived best in VIB. VIB supplemented with bovine serum albumin was used as transport medium in a community-wide surveillance of febrile respiratory disease for influenza viruses. Unfrozen throat swab specimens were placed in VIB and stored at 4 degrees C for up to 5 days without effect on isolation frequencies of either type A or type B influenza virus or type 1 or type 3 parainfluenza virus. Comparison of indirect immunofluorescence with hemadsorption for detection of type A influenza virus in rhesus monkey kidney cultures revealed a requirement for at least five fluorescing cells to eliminate false positive indirect immunofluorescence tests and at least 3 days of incubation to eliminate false negative tests when compared with hemadsorption at later times. Detection frequencies for the two methods after 2 and 3 days of incubation were not significantly different.
D003470:Culture Media; D005455:Fluorescent Antibody Technique; D006382:Hemadsorption; D006801:Humans; D009980:Influenza A virus / Q000302:isolation & purification; D007251:Influenza, Human / Q000382:microbiology*; D009975:Orthomyxoviridae / Q000302:isolation & purification*; D010222:Parainfluenza Virus 1, Human / Q000302:isolation & purification; D010224:Parainfluenza Virus 3, Human / Q000302:isolation & purification; D018184:Paramyxoviridae Infections / Q000382:microbiology*; D018108:Respirovirus / Q000302:isolation & purification*; D012710:Serum Albumin, Bovine; D014776:Virus Cultivation / Q000379:methods*
D003470:Culture Media; D012710:Serum Albumin, Bovine