Chemical analysis of the hyphal wall of Schizophyllum commune.
1. Purified hyphal wall fragments of Schizophyllum commune are analysed and shown to consist of glucose (67.6%), mannose (3.4%), xylose (0.2%), (N-acetyl)glucosamine (12.5%), amino acids (6.4%) and some lipid material (3.0%). 2. The previously proposed structures of two glucans located at the hyphal wall surface (Wessels et al. (1972) Biochim. Biophys. Acta 273, 346-358) were essentially confirmed using methylation analysis. The mucilaginous glucan consists of 1,3-linked beta-glucan chains with branches of single glucose units attached by beta-1,6 linkages on every third unit, on average, along the chain. The alkali soluble S-glucan is an exclusively 1,3-linked alpha-glucan. 3. The alkali-insoluble R-glucan, occurring in close association with chitin, in the inner wall layer, has been characterised by methylation analysis, X-ray diffraction, enzymatic hydrolysis with purified exo-beta-1,3-glucanase and Smith degradation. It appears to be a highly branched beta-1,3,beta-1,6-glucan and a model of this glucan is proposed. Certain parts of this highly insoluble R-glucan bear a close structural similarity to the mucilaginous glucan present at the outer wall surface and in the medium.
D000363:Agaricales / Q000032:analysis*; D000468:Alkalies; D000596:Amino Acids / Q000032:analysis; D002473:Cell Wall / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D002686:Chitin / Q000032:analysis; D005944:Glucosamine / Q000032:analysis; D005947:Glucose / Q000031:analogs & derivatives / Q000032:analysis; D006601:Hexoses / Q000032:analysis; D008055:Lipids / Q000032:analysis; D008745:Methylation; D010084:Oxidation-Reduction; D010504:Periodic Acid; D011134:Polysaccharides / Q000032:analysis*; D012567:Schizophyllum / Q000032:analysis* / Q000648:ultrastructure; D014961:X-Ray Diffraction
D000468:Alkalies; D000596:Amino Acids; D006601:Hexoses; D008055:Lipids; D011134:Polysaccharides; D010504:Periodic Acid; D002686:Chitin; D005947:Glucose; D005944:Glucosamine