title: Collagen production by cultures containing multinucleated cells derived from synovial fibroblasts.
abstract: Multinucleate cells are found frequently in rheumatoid synovium. In this study, polyethylene glycol was used to fuse rabbit synovial fibroblasts. Approximately 40% of the cells developed more than one nucleus in a 24 hour period, during which time cell membranes had increased permeability. In cultures containing multinucleate cells, 3H-thymidine incorporation was depressed for 24 hours although 3H-leucine incorporation into TCA precipitable material was unaffected; autoradiography showed that depression of 3H-thymidine continued for at least 4 days. Collagenase production by cultures containing fused cells was increased more than 10-fold over control cultures during a 28 day period.
mesh_terms: D000818:Animals; D001345:Autoradiography; D002459:Cell Fusion / Q000187:drug effects; D002467:Cell Nucleus; D002478:Cells, Cultured; D005347:Fibroblasts / Q000201:enzymology* / Q000378:metabolism / Q000648:ultrastructure; D007930:Leucine / Q000378:metabolism; D003012:Microbial Collagenase / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000494:pharmacology; D011817:Rabbits; D011868:Radioisotopes; D012413:Rubidium / Q000378:metabolism; D013582:Synovial Fluid / Q000166:cytology*; D013936:Thymidine / Q000378:metabolism
chemical_list: D011868:Radioisotopes; D011092:Polyethylene Glycols; D003012:Microbial Collagenase; D007930:Leucine; D012413:Rubidium; D013936:Thymidine