Sensitive silica gel-packed flowcell for fluorometric detection of aflatoxins by high pressure liquid chromatography.
Aflatoxins B1, B2, G1, and G2 were quantitatively detected by high pressure liquid chromatography on a 5 micronm Lichrosorb column, using a Lichrosorb-packed flowcell in the fluorometric detector. The relationship between peak height and the amount injected was linear only up to about 2 ng but showed a linear loglog relationship. Methods for constructing and packing the flowcell are given. A guard column and venting valve were used to minimize deterioration of the analytical column and the adsorbent-packed flowcell. The method was applied to a peanut butter extract, although with the cleanup procedure used, the life expectancy of the flowcell is limited.
D000348:Aflatoxins / Q000032:analysis*; D010367:Arachis / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D005782:Gels; D008722:Methods; D012822:Silicon Dioxide; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*
D000348:Aflatoxins; D005782:Gels; D012822:Silicon Dioxide