[Use of the reversed passive hemagglutination in detection of Clostridium botulinum type A, B, and E toxin (author's transl)].
With the reversed passive hemagglutination technique it is possible to detect minimal amounts of botulinum type A, B and E toxins (s. Tab. 3). The antisera are used were prepared by foot--pad injection of rabbits with purified toxoids in Freund's complete adjuvant (s. Tab. 1). Antitoxin globulin were prepared from rabbit antisera with (NH4)2SO4 to 50%. Formalinized and tanned human erythrozytes were sensitized with these specific antitoxin globulins. Only slight cross reactions ere encountered between the type A, B, and E antiglobulin sensitized cells and culture filtrates of C. butyricum, C. sporogenes (type A and B antiglobulin only) and C. perfringens type C (s. Tab. 4).
D000907:Antibodies, Bacterial; D001427:Bacterial Toxins / Q000032:analysis*; D003013:Clostridium / Q000276:immunology; D003014:Clostridium botulinum / Q000276:immunology*; D003016:Clostridium perfringens / Q000276:immunology; D003429:Cross Reactions; D004912:Erythrocytes / Q000276:immunology; D006386:Hemagglutination Tests; D006801:Humans; D007114:Immunization
D000907:Antibodies, Bacterial; D001427:Bacterial Toxins
Der Nachweis der Botulinustoxine A, B und E mit Hilfe der passiven Hämagglutination.