A new method for measurement of cyclic AMP phosphodiesterase activity.
The extreme sensitivity of chicken muscle fructose 1,6-bisphosphatase to inhibition by 5'-AMP has been utilized to develop a new method for the assay of cAMP phosphodiesterase activity. In this method, the substrate (cAMP) is first incubated with phosphodiesterase and the amount of 5'-AMP formed is then determined by measuring the degree of inhibition of fructose 1'6-bisphosphatase activity. The present method conveniently employs the spectrophotometric technique and is sensitive enough to detect the conversion of 50 pmol of cAMP to 5'-AMP in 1 ml of reaction mixture. This method is considered particularly valuable for those laboratories that are not equipped with facilities for measuring radioactivity.
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000032:analysis*; D000659:AMP Deaminase / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000494:pharmacology; D000818:Animals; D002417:Cattle; D002645:Chickens; D000242:Cyclic AMP / Q000494:pharmacology; D005260:Female; D006597:Fructose-Bisphosphatase / Q000037:antagonists & inhibitors; D007700:Kinetics; D008722:Methods; D009132:Muscles / Q000201:enzymology; D013053:Spectrophotometry
D000249:Adenosine Monophosphate; D000242:Cyclic AMP; D006597:Fructose-Bisphosphatase; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D000659:AMP Deaminase