Studies on rat ovarian receptors for lutropin (luteinizing hormone). Applicability of radioimmunoassay to measure lutropin bound to receptors.
Measurement of receptor-bound unlabelled physiologically active lutropin (luteinizing hormone, LH) was possible by a modified radioimmunoassay. The conventional radioimmunoassay conducted at 4 degrees C was inadequate, whereas the modified assay performed at 37 degrees C could measure receptor-bound lutropin. The radioimmunoassay at 37 degrees C takes only 36h for completion compared with 5-7 days at 4 degreesC. The sensitivity and range of dose-response curves are, however, unaltered. The validity of the technique was established by a number of criteria.
D000818:Animals; D005260:Female; D007986:Luteinizing Hormone / Q000032:analysis*; D010053:Ovary / Q000032:analysis* / Q000378:metabolism; D011863:Radioimmunoassay / Q000379:methods; D051381:Rats; D011956:Receptors, Cell Surface / Q000032:analysis*
D011956:Receptors, Cell Surface; D007986:Luteinizing Hormone