Methods for the study of the glycoproteins and proteoglycans of bone using bacterial collagenase. Determination of bone sialoprotein and chondroitin sulphate.
A method has been developed for the solubilization of bone matrix using bacterial collagenase. The soluble macromolecules were separated by a column procedure on DEAE-cellulose and analysed for uronic and sialic acids. Values for the total non-collagenous fraction of bone and its content of bone sialoprotein and chondroitin sulphate were obtained. The method was compared with similar procedures using EDTA extraction and papain digestion.
D000818:Animals; D001857:Bone Matrix / Q000032:analysis*; D001842:Bone and Bones / Q000032:analysis*; D002417:Cattle; D002807:Chondroitin / Q000031:analogs & derivatives*; D002809:Chondroitin Sulfates / Q000032:analysis*; D002848:Chromatography, DEAE-Cellulose; D004492:Edetic Acid; D008722:Methods; D003012:Microbial Collagenase; D010206:Papain; D012795:Sialoglycoproteins / Q000032:analysis*
D012795:Sialoglycoproteins; D002807:Chondroitin; D002809:Chondroitin Sulfates; D004492:Edetic Acid; D010206:Papain; D003012:Microbial Collagenase