title: An RNA transcribed from DNA at the origin of phage fd single strand to replicative form conversion.
abstract: Phage fd DNA complexed with DNA binding protein I was used by Escherichia coli RNA polymerase (nucleoside triphosphate:RNA nucleotidyltransferase, EC 2.7.7.6) to synthesize an RNA at the origin of single strand to double strand replication. The isolated ori-RNA gave a simple fingerprint after nucleolytic digestion and has a length of about 30 nucleotides. The characterization of the oligonucleotides from the nuclease digest and the extension of the ori-RNA with DNA polymerase I and subsequent restriction of the DNA gave its exact localization in the fd genome, and its total sequence was deduced from the known DNA sequence in this region.
mesh_terms: D001435:Bacteriophages / Q000378:metabolism*; D001483:Base Sequence; D004265:DNA Helicases / Q000378:metabolism; D004261:DNA Replication; D004277:DNA, Single-Stranded / Q000096:biosynthesis*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D012367:RNA, Viral / Q000096:biosynthesis*; D014158:Transcription, Genetic
chemical_list: D004277:DNA, Single-Stranded; D012367:RNA, Viral; D012321:DNA-Directed RNA Polymerases; D004265:DNA Helicases