An epithelial cell line with chronic polyoma infection established from a spontaneous mouse pancreatic adenocarcinoma.
The establishment of an epithelial cell line from a mouse pancreatic adenocarcinoma is described. The cell line, designated LTPA, was aneuploid and exhibited many transformed growth properties (rapid growth rate, failure to show density-dependent inhibition of growth, ability to grow in defined medium). A type C oncornavirus was isolated from the culture medium, and electron microscopy also revealed the presence of intracisternal type A particles. LTPA cells carried a persistent polyoma infection which produced only low levels of cytopathic effects. A mycoplasmal contamination was also carried. When injected s.c. into Swiss nu/nu mice, LTPA cells formed ductular structures which were destroyed by inflammatory reactions within 3 weeks.
D000230:Adenocarcinoma / Q000235:genetics / Q000382:microbiology* / Q000648:ultrastructure; D000782:Aneuploidy; D000818:Animals; D002460:Cell Line; D005260:Female; D007181:Inclusion Bodies, Viral; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009174:Mycoplasma / Q000302:isolation & purification; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000382:microbiology; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000382:microbiology* / Q000648:ultrastructure; D011120:Polyomavirus / Q000302:isolation & purification*; D012190:Retroviridae / Q000302:isolation & purification; D014184:Transplantation, Homologous