Transforming activity of Epstein-Barr virus obtained by superinfection of Raji cells.
Epstein-Barr virus obtained by superinfection of Raji cells with Epstein-Barr virus recovered from P3HR1 cells (HRI virus) transformed human lymphocytes, but it did not superinfect Raji cells. A human lymphoblastoid cell line, HLB, established by such transformation contained 22 Epstein-Barr virus genomes per cell and Epstein-Barr virus-associated nuclear antigen, and a few cells contained early or viral capsid antigen complexes. Chromosomal analysis revealed that HLB-cells were diploid with normal female karyotypes. Replication of Epstein-Barr virus DNA and inhibition of host cell DNA synthesis were observed in HLB cells after superinfection with HR1 virus.
D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D002472:Cell Transformation, Viral; D004279:DNA, Viral / Q000032:analysis; D004854:Herpesvirus 4, Human / Q000254:growth & development / Q000472:pathogenicity; D007621:Karyotyping; D008214:Lymphocytes / Q000382:microbiology; D014779:Virus Replication
D000956:Antigens, Viral; D004279:DNA, Viral