Immobilization of urea cycle enzymes. I. Characterization of immobilized carbamoylphosphate synthetase and ornithine carbamoyltransferase.
Carbamoylphosphate synthetase (EC 2.7.2.5) and ornithine carbamoyl-transferase (EC 2.1.3.3) extracted from frog liver were successfully immobilized on CNBr-activated Sepharose 4B. The immobilized preparation had a better stability towards heat. The apparent Michaelis constant values for N-acetylglutamate, ammonia, and ATP were not significantly changed by immobilization.
D000818:Animals; D001001:Anura; D002222:Carbamoyl-Phosphate Synthase (Ammonia); D003488:Cyanogen Bromide; D004800:Enzymes, Immobilized; D066298:In Vitro Techniques; D008099:Liver / Q000201:enzymology; D009954:Ornithine Carbamoyltransferase; D010770:Phosphotransferases; D011892:Rana catesbeiana; D012685:Sepharose
D004800:Enzymes, Immobilized; D012685:Sepharose; D009954:Ornithine Carbamoyltransferase; D010770:Phosphotransferases; D002222:Carbamoyl-Phosphate Synthase (Ammonia); D003488:Cyanogen Bromide