title: Incorporation of 14C-labelled amino acids into corticotrophin-like intermediate lobe peptide and alpha-melanocyte-stimulating hormone by the rat pituitary neurointermediate lobe in vitro, and the identification of four new pars intermedia peptides.
abstract: At least seven radioactive peptides, which fractionated on Biogel P6, were found in rat neurointermediate lobes after incubation for 6 h with [14C]proline. Only three of these could be tentatively identified; one as alpha-melanocyte-stimulating hormone (alpha-MSH) and two as forms of corticotrophin-like intermediate lobe peptide (CLIP). One other cross-reacted partially with a beta-malanocyte-stimulating hormone (beta-MSH) antiserum, was acidically charged and eluted on Biogel P6 in roughly the same position as ACTH. The other three peptides showed no resemblance to alpha-MSH, CLIP, beta-MSH or ACTH.
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000031:analogs & derivatives / Q000096:biosynthesis; D000818:Animals; D008297:Male; D009074:Melanocyte-Stimulating Hormones / Q000096:biosynthesis*; D009924:Organ Culture Techniques; D010452:Peptide Biosynthesis; D010902:Pituitary Gland / Q000378:metabolism*; D011392:Proline / Q000378:metabolism; D051381:Rats
chemical_list: D000324:Adrenocorticotropic Hormone; D009074:Melanocyte-Stimulating Hormones; D011392:Proline