Studies on viral DNA protein complexes isolated at different times after infection of monkey kidney cells with simian virus 40.
The major protein components of the DNA complex, isolated from SV40-infected monkey cells, are the major viral structural polypeptide VP1 and cellular histones. At early times (24 h) after infection, VP1 is present in large amounts relative to histones, whereas at late times (48 h), the complex contains mostly histones. The amount of VP1 in the complex can be correlated to the amount of "free" VP1 present in the cells, i.e. VP1 not yet incorporated into virus particles. At early times about 40% of VP1 is "free" VP1; at late times, most of the VP1 is incorporated into virus particles. In contrast, viral DNA is produced in huge excess and only about 13% is incorporated into virions. In agreement with the above result, we find that only about 16% of the DNA in the DNA complex can be chased into virions. There is, apparently, no turnover of newly synthesized VP1 that is associated with the DNA complex at late times after infection.
D000818:Animals; D002478:Cells, Cultured; D004279:DNA, Viral / Q000096:biosynthesis* / Q000302:isolation & purification; D000882:Haplorhini; D006657:Histones / Q000096:biosynthesis; D007668:Kidney / Q000166:cytology; D013539:Simian virus 40 / Q000378:metabolism; D013997:Time Factors; D014764:Viral Proteins / Q000096:biosynthesis*; D014779:Virus Replication
D004279:DNA, Viral; D006657:Histones; D014764:Viral Proteins