title: Effect of dibutyryl cyclic adenosine monophosphate on active amino acid transport in Streptomyces hydrogenans.
abstract: The active uptake of 2-aminoisobutyric acid (AIB) and several other amino acids in resting cells of Streptomyces hydrogenans was found to be stimulated by exogenously added adenosine cyclic monophosphate (cAMP). The uptake of glycerol, sorbose, and pyrimidine nucleosides remained unaffected. Among the various cAMP derivatives tested, the dibutyryl derivative was found to be most effective, followed by monobutyryl cAMP, and cAMP. Dibutyryl cGMP was also found to stimulate AIB transport, and its effectivity was as good as that of dibutyryl cAMP. The effect of dibutyryl cAMP is time dependent and attains its maximum after 40--60 min of incubation at 30 degrees C in K-Na-phosphate buffer. Dibutyryl cAMP-dependent transport stimulation has a high temperature coefficient and is prevented by rifamycin SV or chloramphenicol. The rate of leucine incorporation into protein was rapidly increased upon addition of dibutyryl cAMP. Kinetic studies reveal that the stimulation of AIB transport is characterized by an increase in maximum uptake rate and an unaltered apparent Michaelis constant. Analysis of the unidirectional fluxes show that both influx and efflux are enhanced by dibutyryl cAMP. It is concluded that exogenous dibutyryl cAMP stimulates de novo synthesis of certain proteins including the transport catalysts for various amino acids.
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D000621:Aminoisobutyric Acids / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis; D001693:Biological Transport, Active / Q000187:drug effects; D003994:Bucladesine / Q000494:pharmacology*; D002701:Chloramphenicol / Q000494:pharmacology; D000242:Cyclic AMP / Q000494:pharmacology; D003562:Cytidine / Q000378:metabolism; D003995:Dibutyryl Cyclic GMP / Q000494:pharmacology; D005990:Glycerol / Q000378:metabolism; D012294:Rifamycins / Q000494:pharmacology; D013013:Sorbose / Q000378:metabolism; D013302:Streptomyces / Q000378:metabolism*; D013936:Thymidine / Q000378:metabolism; D014529:Uridine / Q000378:metabolism
chemical_list: D000596:Amino Acids; D000621:Aminoisobutyric Acids; D001426:Bacterial Proteins; D012294:Rifamycins; D003995:Dibutyryl Cyclic GMP; D003562:Cytidine; D003994:Bucladesine; D002701:Chloramphenicol; D000242:Cyclic AMP; D013013:Sorbose; D005990:Glycerol; D013936:Thymidine; D014529:Uridine