Densitometric quantitation of neutral lipids on ammonium sulfate impregnated thin-layer chromatograms.
A procedure is described which extends the densitometric quantitation of phospholipids on ammonium sulfate impregnated thin-layer chromatograms by Gluck et al. to include total lipid, free and esterified cholesterol, free fatty acid and triglyceride. Lipids separated on thin-layer plates containing silica gel G impregnated with ammonium sulfate were charred upon heating and absorbance was measured densitometrically. Thus, the necessity of spraying or submersing in a charring agent was eliminated, uniform charring became possible, and quantitation over a wider range of sample sizes than most densitometric procedures was obtained. One linear relationship existed for concentrations of standards over the range of 0.0-3.0 mug and another line from 4.0-50.0 mug. Both accuracy and precision of the method were highly reliable.
D000645:Ammonium Sulfate; D002784:Cholesterol / Q000032:analysis; D002788:Cholesterol Esters / Q000032:analysis; D002855:Chromatography, Thin Layer; D003720:Densitometry; D005227:Fatty Acids / Q000032:analysis; D005230:Fatty Acids, Nonesterified / Q000032:analysis; D005782:Gels; D008055:Lipids / Q000032:analysis*; D010743:Phospholipids / Q000032:analysis; D012822:Silicon Dioxide; D014280:Triglycerides / Q000032:analysis
D002788:Cholesterol Esters; D005227:Fatty Acids; D005230:Fatty Acids, Nonesterified; D005782:Gels; D008055:Lipids; D010743:Phospholipids; D014280:Triglycerides; D012822:Silicon Dioxide; D002784:Cholesterol; D000645:Ammonium Sulfate