[Comparative study of B890 pigment-lipoprotein complexes from sulfur (Chromatium minutissimum) and non-sulfur (Rhodopseudomonas palustris) purple photosynthesizing bacteria].
Pigment-lipoprotein B890 complexes containing reaction center and "light-focusing" bacteriochlorophyll a were isolated from photosynthetic membranes of sulfur (Chromatium minutissimum) and non-sulfur (Rhodopseudomonas palustris) purple bacteria after the treatment with Triton X-100. The molecular weights of complexes were evaluated using several methods (200 000-300 000). By means of electron microscopy the sizes of complexes were found to be about 80 A. On the air-water interface hexagonal packing of complexes was observed. The chemical compositions of complexes are very similar except bacteriochlorophyll a whose specific content is somewhat higher in Chromatium minutissimum. The protein composition of complexes was studied and the molecular weights of proteins were estimated by SDS-gel electrophoresis. The results obtained show significant similarities in molecular organization of B890 complexes isolated from sulfur (Chromatium minutissimum) and non-sulfur (Rhodopseudomonas palustris) purple bacteria.
D001426:Bacterial Proteins; D001429:Bacteriochlorophylls; D055598:Chemical Phenomena; D002621:Chemistry; D002844:Chromatium / Q000032:analysis*; D003574:Cytochrome c Group; D008074:Lipoproteins; D008854:Microscopy, Electron; D008970:Molecular Weight; D010860:Pigments, Biological; D012241:Rhodopseudomonas / Q000032:analysis*; D013045:Species Specificity; D013057:Spectrum Analysis
D001426:Bacterial Proteins; D001429:Bacteriochlorophylls; D003574:Cytochrome c Group; D008074:Lipoproteins; D010860:Pigments, Biological
Sravnitel'noe issledovanie pigment-lipoproteinovykh kompleksov B890 is sernykh (Chromatium minutissimum) i nesernyky (Rhodopseudomonas palustris) purpurnykh fotosinteziruiushchikh bakteriĭ.