A greatly simplified method of establishing B-lymphoblastoid cell lines.
Ten lymphoblastoid cell lines were established by Epstein-Barr virus-induced transformation directly from 0.04 to 0.15 ml of peripheral whole blood of one patient with xeroderma pigmentosum and four normal healthy adults. All these lines expressed B-lymphocyte characteristics. The advantages of this method are: (a) only a few drops of blood are required for establishing a permanent line; (b) damage and loss of cells in separation procedures are minimal; and (c) the method is simple, reliable, and applicable, if desired, to any patient, even babies.
D000328:Adult; D001402:B-Lymphocytes / Q000473:pathology*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D004854:Herpesvirus 4, Human; D006801:Humans; D008722:Methods; D014983:Xeroderma Pigmentosum / Q000097:blood