Characteristics of modulators and substrates binding to rat liver adenylate kinase.
The binding of adenine nucleotides to liver adenylate kinase was dependent on Mg2+ ions. Citric acid enhanced the binding of all metal-chelated radioactive nucleotides and indicated two observable binding sites for Mg3H-ADP and Mg3-ATP and one-half binding site for Mg3H-AMP. Two binding sites of Mg3H-ADP and one binding site for Mg3H-ATP were also observed in the absence of citric acid. Stoichiometric binding of 14C-citric acid to liver adenylate kinase varied with additions of different nucleotides. AMP prevented whereas ADP and ATP enhanced the binding of 14C-citric acid.
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000249:Adenosine Monophosphate / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000263:Adenylate Kinase / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D002951:Citrates / Q000494:pharmacology; D007700:Kinetics; D008024:Ligands; D008099:Liver / Q000201:enzymology*; D008274:Magnesium / Q000494:pharmacology; D010770:Phosphotransferases / Q000378:metabolism*; D011485:Protein Binding; D051381:Rats
D002951:Citrates; D008024:Ligands; D000249:Adenosine Monophosphate; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D010770:Phosphotransferases; D000263:Adenylate Kinase; D008274:Magnesium