[Structure and function of virus-induced antigens in cultured cells infected with Merek's disease and turkey herpes viruses. II. Isolation of intracellular antigens from infected cells (author's transl)].
Virus-induced antigens were isolated from MDV and HVT-infected cells by salt extraction with 3M KCl and purified by Con.A chromatography and isoelectric focusing. Electrophoretic analysis of 35S-methionine, 3H-fucose and 32P-orthophosphate labeled antigens revealed 7 different polypeptides, two of them containing labeled carbohydrates, and one phospholipid component. The isolated virus-induced antigens from infected cells were identified as serologically active membrane complexes carrying common antigenic determinants of MDV and HVT. They were able to bind to virus-neutralizing immunglobulins as shown by antibody binding tests. Summarizing the presented analytic and serologic findings it was proposed to classify MDV and HVT as different serotypes of a common Marek's disease virus group.
D000818:Animals; D000956:Antigens, Viral / Q000302:isolation & purification*; D002460:Cell Line; D002478:Cells, Cultured; D002642:Chick Embryo; D003429:Cross Reactions; D003593:Cytoplasm / Q000276:immunology; D004372:Ducks; D006564:Herpesviridae / Q000145:classification / Q000276:immunology*; D008381:Herpesvirus 2, Gallid / Q000145:classification / Q000276:immunology*; D008380:Marek Disease / Q000517:prevention & control; D008715:Methionine / Q000378:metabolism; D011947:Receptors, Antigen, B-Cell; D014611:Vaccination
D000956:Antigens, Viral; D011947:Receptors, Antigen, B-Cell; D008715:Methionine
Struktur und Funktion Virus-induzierter Antigene in Kulturzellen nach der Infektion mit dem Marek- und Putenherpesvirus II. Isolierung intrazellulärer Antigene aus infizierten Zellen.