Mechanism of poliovirus inactivation by ammonia.
Poliovirus inactivation by ammonia causes a slight reduction in the sedimentation coefficients of viral particles, but has no detectable effect on either the electrophoretic pattern of viral capsid proteins or the isoelectric points of inactivated particles. These virions still attach to cells, but are unable to repress host translation or stimulate the synthesis of detectable amounts of viral RNA. Although ammonia has no detectable effect on naked poliovirus RNA, it causes cleavage of this RNA when still within viral particles. Therefore, the RNA genome appears to be the only component of poliovirus significantly affected by ammonia.
D000641:Ammonia / Q000494:pharmacology*; D002213:Capsid / Q000378:metabolism; D005814:Genes, Viral / Q000187:drug effects*; D017955:Poliovirus / Q000187:drug effects*; D014176:Protein Biosynthesis; D011487:Protein Conformation / Q000187:drug effects; D012367:RNA, Viral / Q000378:metabolism*; D014764:Viral Proteins / Q000096:biosynthesis
D012367:RNA, Viral; D014764:Viral Proteins; D000641:Ammonia