The effect of adenosine-3':5'-cyclic monophosphate on the mitotic rate in regenerating Dugesia dorotocephala.
Regenerating posterior sections of the flatworm, Dugesia dorotocephala, were treated with varying concentrations of 5'-adenosine monophosphate (AMP), cyclic AMP (cAMP) and dibutyryl cyclic AMP (Bt2cAMP) for 24 h. M/2000 colchicine was added to the medium during the final 4 h of treatment to collect mitotic figures. The mitotic rate was significantly increased at 0.5, 0.1 and 0.01 mM concentrations of Bt2-cAMP. While Bt2cAMP and cAMP produced comparable results at 0.01 mM, only the Bt2-cAMP-treated organisms exhibited a significantly higher mitotic rate at the 0.1 mM concentration. Theophylline and sodium butyrate did not evoke any stimulatory effect on mitotic rate.
D000249:Adenosine Monophosphate / Q000494:pharmacology*; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology*; D002087:Butyrates / Q000494:pharmacology; D000242:Cyclic AMP / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D008938:Mitosis / Q000187:drug effects*; D010986:Platyhelminths / Q000187:drug effects* / Q000502:physiology; D012038:Regeneration; D013806:Theophylline / Q000494:pharmacology
D002087:Butyrates; D000249:Adenosine Monophosphate; D003994:Bucladesine; D013806:Theophylline; D000242:Cyclic AMP