title: DNA-directed in vitro synthesis of Escherichia coli beta-isopropylmalate dehydrogenase.
abstract: The in vitro synthesis of beta-isopropylmalate dehydrogenase (EC 1.1.1.85), an enzyme involved in leucine biosynthesis, has been obtained using as template DNA from the hybrid plasmid (pLC1) which contains the Escherichia coli leucine operon. Enzyme synthesis in vitro is stimulated about 2-fold by guanosine-5'-diphosphate-3'-diphosphate and inhibited about 60% by 2 X 10(-4) M L-leucine.
mesh_terms: D000429:Alcohol Oxidoreductases / Q000096:biosynthesis*; D000242:Cyclic AMP / Q000494:pharmacology; D004269:DNA, Bacterial / Q000378:metabolism*; D003998:Dicarboxylic Acids; D004926:Escherichia coli / Q000201:enzymology*; D006159:Guanosine Tetraphosphate / Q000494:pharmacology; D007700:Kinetics; D007930:Leucine / Q000378:metabolism; D010957:Plasmids; D014176:Protein Biosynthesis / Q000187:drug effects; D013698:Templates, Genetic; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D004269:DNA, Bacterial; D003998:Dicarboxylic Acids; D006159:Guanosine Tetraphosphate; D000242:Cyclic AMP; D000429:Alcohol Oxidoreductases; D007930:Leucine