Characterization of the myosin-phosphorylating system in normal murine astrocytes and derivative sv40 wild-type and A-mutant transformant.
Myosin and myosin light-chain kinase have been isolated and characterized from small quantities of normal and SV40-transformed, murine astrocytic neuroglial cells in culture and from intact normal mouse brain. Sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis of the astrocyte myosins revealed a heavy chain of 200,000 daltons and two light chains of 20,000 and 15,000 daltons. These myosins are similar to other cytyplasmic myosins. The astrocyte 20,000-dalton light chain can be phosphorylated by an endogenous myosin light-chain kinase which has properties similar to those of the myosin light-chain kinase found in human platelets. No differences were detected in either the astrocyte myosins or myosin light-chain kinases between (a) the normal and transformed cells, (b) the transformed cells grown at the permissive and nonpermissive temperatures, or (c) the SV40 wild-type and A-mutant transformants.
D000199:Actins / Q000032:analysis; D000251:Adenosine Triphosphatases / Q000378:metabolism; D001253:Astrocytes / Q000032:analysis*; D002460:Cell Line; D002472:Cell Transformation, Viral; D009218:Myosins / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*; D009457:Neuroglia / Q000032:analysis*; D010710:Phosphates / Q000378:metabolism; D010770:Phosphotransferases / Q000302:isolation & purification / Q000378:metabolism; D013539:Simian virus 40
D000199:Actins; D010710:Phosphates; D010770:Phosphotransferases; D000251:Adenosine Triphosphatases; D009218:Myosins