title: Isolation and characterization of the opioid peptides from rat pituitary: beta-lipotropin.
abstract: Rat beta-lipotropin was isolated from 40 anterior pituitaries using high efficiency chromatography columns and sensitive fluorometric methods. The beta-lipotropin was characterized as to molecular weight, amino acid composition, and generation of opioid activity by trypsinization. Other opioid peptides, as well as a precursor to opioid peptides with a molecular weight larger than that of beta-lipotropin, were observed in this tissue.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D004723:Endorphins / Q000302:isolation & purification; D004745:Enkephalins / Q000302:isolation & purification; D008297:Male; D008970:Molecular Weight; D010902:Pituitary Gland / Q000032:analysis*; D010903:Pituitary Gland, Anterior / Q000032:analysis*; D051381:Rats; D011957:Receptors, Opioid / Q000378:metabolism; D014357:Trypsin / Q000378:metabolism; D008083:beta-Lipotropin / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D000596:Amino Acids; D004723:Endorphins; D004745:Enkephalins; D011957:Receptors, Opioid; D008083:beta-Lipotropin; D014357:Trypsin