[The densitometric evaluation of lipoprotein electrophoresis on acetate foil and agar-agarose gel].
By means of cholesterol feeding on rabbits the change of the beta- and pre-beta-lipoprotein relations is observed by densitometric evaluation of lipoprotein electrophoreses on cellulose acetate foils and in agar-agarose gel. At the same time the changes of the cholesterol and triglyceride content of the sera are determined. The agar-agarose-gel electrophoreses prove to be more suitable for the densitometric evaluation compared with the electrophoreses on cellulose acetate foils, since they give more exact results. A reason for this is the worse demarcability of the pre-beta-lipoprotein proportion in the curves of the densitometre of acetate foils. The percental distributions of lipoprotein in patients of different types of HLP are determined and with the help of the results the evidence of the visual judgment of the lipoprotein electrophoreses is discussed.
D000818:Animals; D002784:Cholesterol / Q000032:analysis; D003720:Densitometry; D004586:Electrophoresis / Q000379:methods*; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D004588:Electrophoresis, Cellulose Acetate / Q000379:methods*; D006801:Humans; D006949:Hyperlipidemias / Q000175:diagnosis; D008074:Lipoproteins / Q000032:analysis*; D008077:Lipoproteins, LDL / Q000032:analysis; D011817:Rabbits
D008074:Lipoproteins; D008077:Lipoproteins, LDL; D002784:Cholesterol
Zur densitometrischen Auswertung von Lipoproteinelektrophoresen auf Azetatfolien und Agar-Agarosegel