Release of glycopeptides and mucopolysaccharides from ascites hepatoma cells by tryptic treatment.
Two types of ascites hepatoma cells, AH 66 and AH 130 FN, were treated with trypsin to observe the release of complex carbohydrates constituting the plasma membranes. From AH 66 cells, mucopolysaccharide (heparan sulfate) was preferentially released. From AH 130 FN cells, N-glycosidic glycopeptides were preferentially released whereas no mucopolysaccharide (chondroitin sulfate A) was released.
D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000378:metabolism*; D002478:Cells, Cultured; D005260:Female; D005944:Glucosamine / Q000378:metabolism; D006020:Glycopeptides / Q000378:metabolism*; D006025:Glycosaminoglycans / Q000378:metabolism*; D006497:Heparan Sulfate / Q000378:metabolism; D008113:Liver Neoplasms / Q000378:metabolism*; D051381:Rats; D013431:Sulfates / Q000378:metabolism; D014357:Trypsin
D005944:Glucosamine; D006020:Glycopeptides; D006025:Glycosaminoglycans; D006497:Heparan Sulfate; D013431:Sulfates; D014357:Trypsin