title: Association of vesicular stomatitis virus proteins with HeLa cell membranes and released virus.
abstract: The association of vesicular stomatitis virus proteins with intracellular and plasma membranes was examined by pulse and pulse-chase labeling of virus-infected HeLa cells with [35S]methionine and separation of cell homogenates into three major membrane fractions in discontinuous sucrose gradients. The glycoprotein G was primarily associated with rough endoplasmic reticulum-like membranes after short radioactive pulses (2 to 4 min) but accumulated in the plasma membrane-enriched fraction and the smooth internal membrane fraction with longer pulse or chase periods. The nucleocapsid protein N and the matrix protein M accumulated in the rough endoplasmic reticulum and plasma membrane-like fractions but not in the smooth internal membrane fraction. Only a fraction (35 to 40%) of the viral protein synthesized during a short pulse in the mid-cycle of infection was apparently utilized in released virus. The newly synthesized virus proteins first appeared in released virus in the order: M, N and L, and G.
mesh_terms: D002213:Capsid / Q000032:analysis; D002462:Cell Membrane / Q000032:analysis*; D004721:Endoplasmic Reticulum / Q000032:analysis*; D006023:Glycoproteins / Q000032:analysis*; D006367:HeLa Cells; D014721:Vesicular stomatitis Indiana virus / Q000032:analysis*; D014764:Viral Proteins / Q000032:analysis* / Q000096:biosynthesis
chemical_list: D006023:Glycoproteins; D014764:Viral Proteins