Agarose gel filtration of concentrated fecal extracts containing virus-like particles associated with hepatitis A.
Virus-like particles shown to be associated with hepatitis A infection were recently visualized by immune electron microscopy in human and chimpanzee acute-phase fecal extracts. Agarose gel filtration of concentrated chimpanzee fecal extracts containing those 27-nm diameter particles separated more than 99% of the high molecular weight fecal impurities into two major peaks as determined by absorbance at 280 and 260 nm. The hepatitis A-associated particles were found between these two peaks by both immune electron microscopy and a new immunoradiometric assay.
D000818:Animals; D000937:Antigen-Antibody Reactions; D002847:Chromatography, Agarose; D005243:Feces / Q000382:microbiology*; D006506:Hepatitis A / Q000382:microbiology / Q000662:veterinary*; D006507:Hepatovirus / Q000302:isolation & purification*; D008854:Microscopy, Electron; D002679:Pan troglodytes / Q000382:microbiology*