Selective precipitation of very low density lipoproteins from fasting sera by heparin and manganese ions.
Complete and discrete precipitation of very low density lipoproteins (VLDL) from fasting sera required heating at 40 degrees C for 60 min in the presence of 0.05 M manganous ions and heparin in a concentration dependent upon the VLDL content. The minimum polyanion concentration, employed with VLDL contents less than 1.0 g/l, was 85 mg/l; an increment of 5 mg/l was required for each 1.0 g/l increase in VLDL content. The products isolated by this procedure had electrophoretic, immunochemical and ultracentrifugal characteristics skin to those of native VLDL and were slightly contaminated with albumin. The reaction conditions did not affect adversely the subsequent precipitation of low density lipoproteins.
D005215:Fasting; D006493:Heparin; D006801:Humans; D008079:Lipoproteins, VLDL / Q000097:blood* / Q000302:isolation & purification; D008345:Manganese; D008722:Methods; D013696:Temperature; D013997:Time Factors
D008079:Lipoproteins, VLDL; D008345:Manganese; D006493:Heparin