[Accumulation of phosphate ions in biological materials, phosphate ion diffusion and electron microscopical investigations of phosphohydrolases (author's transl)].
Isolated unfixed nuclei of mouse liver accumulate different amounts of phosphate ions dependent on pH, temperature and concentration of phosphate ions. At 37 degrees C and pH = 7.2, 8.5 X 10(9) binding places and a stability constant of 2.0 +/- 0,5 . 10(4) l/mol were calculated, the data at 30 degrees C and pH = 6.5 are 4 X 10(9) Nucleus-1 and 6 X 10(3) l/mol. Nuclei fixed by formaldehyde-ethanol or glutaraldehyde do not accumulated phosphate ions. Under conditions of nearly undisturbed diffusion phosphate ions taken up by the nuclei and precipitated by the help of lead ions are detected electron microscopically in the nuclear envelope, preferently in intracisternal space. Nucleoprotein structures do not show enrichments of crystals. These structures are also not stained after precipitation of phosphate ions under conditions of diminuation of diffusion. A possible mode of phosphate binding in cell nuclei and the influence of adsorbed phosphate ions on localization of enzymatic activities are discussed.
D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure; D004058:Diffusion; D006651:Histocytochemistry; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010710:Phosphates / Q000378:metabolism*; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*
D010710:Phosphates; D010744:Phosphoric Monoester Hydrolases
Phosphationenaufnahme durch biologische Materialien, Phosphationendiffusion und elektronenoptischer Phosphohydrolase-Nachweis.