The syntheses of alpha-D-glucopyranose-1-phosphates labelled with 18O in the phosphoryl or ester oxygen positions.
Phosphoryl-oxygen-labelled alpha-D-glucopyranose-1-phosphate (Glc1P) has been prepared by the hydrolysis in 18O-enriched water of alpha-D-glucopyranose-1,2-cyclic phosphate catalyzed by extracts of Saccharomyces fragilis. Ester-oxygen-labelled alpha-D-galactopyranose-1-phosphate has been prepared by galactokinase-(EC 2.7.1.6)-catalyzed phosphorylation of D-[1-18O]-galactose by ATP, and it has been isomerized to ester-oxygen-labelled Glc1P using galactose-1-phosphate uridyltransferase (EC2.7.7.12) and UDPgalactose 4-epimerase (EC 5.1.3.2). These labelled glucose phosphates are for use in oxygen-exchange experiments.
D005690:Galactose / Q000378:metabolism; D005958:Glucosephosphates / Q000096:biosynthesis*; D010103:Oxygen Isotopes; D010710:Phosphates; D010770:Phosphotransferases / Q000378:metabolism; D012440:Saccharomyces / Q000378:metabolism; D014534:UDPglucose 4-Epimerase / Q000378:metabolism; D005694:UTP-Hexose-1-Phosphate Uridylyltransferase / Q000378:metabolism
D005958:Glucosephosphates; D010103:Oxygen Isotopes; D010710:Phosphates; D010770:Phosphotransferases; D005694:UTP-Hexose-1-Phosphate Uridylyltransferase; D014534:UDPglucose 4-Epimerase; D005690:Galactose