Type B and type C RNA tumor virus replication in single cells. Electron microscopy with tannic acid.
Simultaneous replication of murine mammary tumor virus (type B) and murine leukemia virus (type C) was demonstrated electron microscopically along continuous stretches of the plasma membrane of single cells in cultures ofthe Mm5mt/c1 cell line. Types B and C virus buds were discriminated in thin sections with the aid of a tannic acid fixative that revealed the type B surface spikes as a homogeneous band of intermediate density and constant width on the surfaces of some buds (type B), whereas others (type C) remained with relatively smooth envelopes. Both types B and C buds may contain morphologically identical horseshoe-shaped nucleoids. Therefore, their identify (type B or C) could be ascertained in thin sections only on the basis of recognition of surface spikes.
D047348:Hydrolyzable Tannins; D007181:Inclusion Bodies, Viral; D009052:Leukemia Virus, Murine / Q000648:ultrastructure*; D008324:Mammary Tumor Virus, Mouse / Q000648:ultrastructure*; D008854:Microscopy, Electron; D014779:Virus Replication
D047348:Hydrolyzable Tannins