Virus isolation test for feline leukemia virus.
A method was developed for detecting feline leukemia virus (FeLV) infection in cats by coculturing separated blood lymphocytes and platelets with clone 81 cells carrying a murine sarcoma genome but free of leukemia virus (S+L-). FeLV caused cell transformation that was easily visible 3-12 days after infection. This method is simple, sensitive, and easily read; it has the advantage of directly detecting infectious FeLV rather than viral antigens.
D000818:Animals; D001792:Blood Platelets / Q000382:microbiology; D002371:Cat Diseases / Q000382:microbiology; D002415:Cats; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D007938:Leukemia / Q000382:microbiology / Q000662:veterinary; D005256:Leukemia Virus, Feline / Q000302:isolation & purification*; D008214:Lymphocytes / Q000382:microbiology; D008722:Methods; D014412:Tumor Virus Infections / Q000382:microbiology*