title: Effect of cyclic 3':5'-AMP derivatives prostaglandins and related agents on human chorionic gonadotropin secretion in human malignant trophoblast in culture.
abstract: The secretion of human chorionic gonadotropin (hCG) is stimulated by addition of N6, O2'-dibutyryl cyclic 3':5'-AMP (dbcAMP) or theophylline to normal term placenta and human malignant trophoblast cells in vitro. To understand better the specificity of this process, malignant trophoblast cultures were incubated with 3':5'-cyclic AMP (cAMP) derivatives, prostaglandins and other agents for 1 to 3 days, and the secretion of radioimmunoassayable hCG was measured. Whereas dbcAMP was the most potent agent in stimulating secretion of hCG, the N6--and O2'-monobutyryl derivatives of cAMP and phosphodiesterase inhibitors (theophylline, papaverine, 3-isobutyl-1-methylxanthine) also increased the secretion of the hormone. A slight increase in hCG secretion was observed following addition of adenine. By contrast, butyrate, cAMP, cyclic 3':5'-GMP (cGMP), dbcGMP, 5'-AMP, adenosine, L-epinephrine and prostaglandins E1, E2, F1a and F2a were ineffective. Particulate fractions from sonicates of malignant trophoblast cultures contained adenylate cyclase activity which was stimulated more than 10-fold by NaF, but not by either catecholamines or prostaglandins. The relatively specific stimulation of hCG secretion suggested that a regulatory process involving cAMP may have physiological significance in the trophoblast.
mesh_terms: D000225:Adenine / Q000494:pharmacology; D000262:Adenylyl Cyclases / Q000378:metabolism; D003994:Bucladesine / Q000494:pharmacology; D002460:Cell Line; D006063:Chorionic Gonadotropin / Q000096:biosynthesis*; D000242:Cyclic AMP / Q000031:analogs & derivatives* / Q000494:pharmacology; D005260:Female; D005459:Fluorides / Q000494:pharmacology; D006801:Humans; D010208:Papaverine / Q000494:pharmacology; D011247:Pregnancy; D011453:Prostaglandins / Q000494:pharmacology*; D013268:Stimulation, Chemical; D013806:Theophylline / Q000494:pharmacology; D014328:Trophoblastic Neoplasms; D014970:Xanthines / Q000494:pharmacology
chemical_list: D006063:Chorionic Gonadotropin; D011453:Prostaglandins; D014970:Xanthines; D003994:Bucladesine; D013806:Theophylline; D010208:Papaverine; D000242:Cyclic AMP; D000262:Adenylyl Cyclases; D000225:Adenine; D005459:Fluorides