Adenosine diphosphoribosylation of certain basic chromosomal proteins in isolated trout testis nuclei.
Isolated trout testis nuclei rapidly incorporate [alpha-32P]NAD+ into chromosomal proteins. Three proteins, very-lysine-rich histone (H1), a specific trout chromosomal protein (H6) and the sperm-specific protamines, incorporate the label as adenosine diphosphoribosyl (ADP-Rib) residues. No significant labeling of the nucleosomal 'core' histones H2A, H2B, H3 and H4 was observed. The linkage of the [32P](ADP-Rib) residues to each protein was very labile at pH values greater than 7.0 but by working at acidic pH and low temperatures the ADP-Rib label could be shown to be covalently bound to protein by gel electrophoresis and ion-exchange chromatography. The [32P]ADP-Rib chains could be removed by digestion with snake venom phosphodiesterase with the formation of AMP and phosphoribosyl-AMP.
D000247:Adenosine Diphosphate Sugars / Q000378:metabolism*; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D002875:Chromosomes / Q000378:metabolism*; D006657:Histones / Q000378:metabolism; D008297:Male; D009243:NAD; D009698:Nucleoproteins / Q000378:metabolism*; D009702:Nucleoside Diphosphate Sugars / Q000378:metabolism*; D011479:Protamines / Q000378:metabolism; D012266:Ribose / Q000378:metabolism; D013737:Testis / Q000378:metabolism*; D014337:Trout
D000247:Adenosine Diphosphate Sugars; D000596:Amino Acids; D006657:Histones; D009698:Nucleoproteins; D009702:Nucleoside Diphosphate Sugars; D011479:Protamines; D009243:NAD; D012266:Ribose