The effect of metiamide on acid secretion stimulated by gastrin, acetylcholine and dibutyryl cyclic adenosine 3',5'-monophosphate in the isolated whole stomach of the rat.
1 An isolated stomach preparation from immature rats is described. The lumen of the stomach was perfused and the hydrogen ion activity of the perfusate recorded continuously. 2 The preparation gave dose-dependent responses to gastrin, acetylcholine and dibutyryl cyclic adenosine 3',5'-monophosphate and these responses were readily reversed on washing out the agonist. 3 The acid secretory response to gastrin was inhibited by metiamide at concentrations of 10(-5) M and 3 X 10(-5)M. 4 The acid secretory responses to acetylcholine and dibutyryl cyclic adenosine 3',5'-monophosphate were not inhibited by concentrations of metiamide up to 10(-3) M. 5 These findings are discussed in relation to the role of histamine in the control of gastric acid secretion.
D000109:Acetylcholine / Q000494:pharmacology*; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology*; D004347:Drug Interactions; D005260:Female; D005750:Gastric Juice / Q000378:metabolism*; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism*; D005755:Gastrins / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D008785:Metiamide / Q000494:pharmacology*; D010418:Pentagastrin / Q000494:pharmacology; D051381:Rats; D013268:Stimulation, Chemical; D013890:Thiourea / Q000031:analogs & derivatives*; D013997:Time Factors
D005755:Gastrins; D008785:Metiamide; D003994:Bucladesine; D010418:Pentagastrin; D013890:Thiourea; D000109:Acetylcholine