Receptors for luteinizing hormone-releasing hormone.
Antisera to luteinizing hormone-releasing hormone (LH-RH) confer on Araldite sections of occasional rat pituitaries moderate immunocytochemical staining to the large secretory granules of gonadotrophs. Treatment of the sections with LH-RH before anti-LH-RH yields strong staining in all animals, irrespective of presence or absence of staining without pretreatment. This enhancement of staining is specific for LH-RH and is a high affinity, saturable reaction. Staining with or without LH-RH pretreatment is absent when anti-LH-RH absorbed with insolubilized LH-RH is used. Staining is inhibited by carboxyterminally-deficient LH-RH, unaffected by aminoterminally deficient LH-RH.
D000311:Adrenal Glands; D000818:Animals; D000918:Antibody Specificity; D002499:Centrifugation, Density Gradient; D003429:Cross Reactions; D003594:Cytoplasmic Granules; D007987:Gonadotropin-Releasing Hormone; D007106:Immune Sera / Q000494:pharmacology; D007124:Immunoenzyme Techniques; D010455:Peptides; D010902:Pituitary Gland / Q000378:metabolism; D051381:Rats; D011956:Receptors, Cell Surface
D007106:Immune Sera; D010455:Peptides; D011956:Receptors, Cell Surface; D007987:Gonadotropin-Releasing Hormone