Glycosaminoglycan synthesis by Wilms' tumor.
Wilms' tumor contains approximately 1 mg hyaluronic acid and approximately 0.3 mg sulfated glycosaminoglycan per g tissue. Minced tumor and cells cultured from the tumor incorporate labeled acetate and glucosamine into hyaluronic acid and sulfated glycosaminoglycans. A particulate enzyme preparation derived from the tumor catalyzed the transfer of GlcUA or GlcNAc from UDP-GlcUA or UDP-GlcNAc at a rate of approximately 20 nmol/hr/mg protein to produce high molecular weight hyaluronic acid chains. The urine and plasma of a Wilms' tumor patient contained approximately 20 mg hyaluronic acid and 8 mg sulfated glycosaminoglycan/100 ml, respectively. It appears that this higher than normal level of circulating glycosaminoglycan is synthesized by the Wilms' tumor.
D002478:Cells, Cultured; D006025:Glycosaminoglycans / Q000032:analysis / Q000096:biosynthesis*; D006801:Humans; D006820:Hyaluronic Acid / Q000096:biosynthesis; D007680:Kidney Neoplasms / Q000201:enzymology / Q000378:metabolism*; D009396:Wilms Tumor / Q000201:enzymology / Q000378:metabolism*
D006025:Glycosaminoglycans; D006820:Hyaluronic Acid