Affinity chromatography of myosin, heavy meromyosin, and heavy meromyosin subfragment one on F-actin columns stabilized by phalloidin.
A method of affinity chromatography based on the trapping of actin filaments within agarose gel beads is described. This method can be used for the purification of myosin and its active proteolytic subfragments, as well as for studies on the interaction between actin and these proteins. Actin columns stabilized by phalloidin bind myosin, heavy meromyosin (HMM), and heavy meromyosin subfragment 1 (HMM-S1) specifically and reversibly. The effect of pyrophosphate and KCl on the dissociation of actomyosin, acto-HMM, or acto-HMM-S1 complex is reported. We also describe the single-step purification of myosin from a crude rabbit psoas muscle extract.
D000199:Actins; D000818:Animals; D002846:Chromatography, Affinity; D011756:Diphosphates; D015879:Myosin Subfragments / Q000302:isolation & purification*; D009218:Myosins / Q000302:isolation & purification*; D010590:Phalloidine; D011189:Potassium Chloride; D011485:Protein Binding; D011817:Rabbits
D000199:Actins; D011756:Diphosphates; D015879:Myosin Subfragments; D010590:Phalloidine; D011189:Potassium Chloride; D009218:Myosins