title: Decreased iodination of the red cell surface following phospholipase C treatment.
abstract: Human red blood cells were treated with phospholipase C from Clostridium welchii. Lipase concentrations which produced less than 1% hemolysis and 10-15% hydrolysis of the membrane phospholipids reduced markedly (greater than 80%) the accessibility of membrane proteins to the external surface as measured by lactoperoxidase-catalyzed iodination.
mesh_terms: D003016:Clostridium perfringens / Q000201:enzymology; D004910:Erythrocyte Membrane / Q000187:drug effects / Q000648:ultrastructure*; D004912:Erythrocytes / Q000648:ultrastructure*; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D007457:Iodine Radioisotopes; D007553:Isotope Labeling; D007784:Lactoperoxidase; D008563:Membrane Lipids / Q000097:blood; D008565:Membrane Proteins / Q000378:metabolism; D010740:Phospholipases / Q000494:pharmacology*; D010743:Phospholipids / Q000097:blood
chemical_list: D007457:Iodine Radioisotopes; D008563:Membrane Lipids; D008565:Membrane Proteins; D010743:Phospholipids; D007784:Lactoperoxidase; D010740:Phospholipases