[Improvements in the technical conditions for the evaluation of an elastolytic activity. The role of tryspin as an activator (author's transl)].
An isotopic assay of elastolytic activity is performed; the iodine-125 ions retained inside the elastin framework were removed by appropriate treatment. This acute substrate enables us to study the role of trypsin on elastase activity and facilitates the study of elastase role in atherosclerosis.
D000818:Animals; D001158:Arteries / Q000378:metabolism; D001161:Arteriosclerosis / Q000378:metabolism*; D004285:Dogs; D004549:Elastin / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007457:Iodine Radioisotopes; D008722:Methods; D010179:Pancreas / Q000378:metabolism; D010196:Pancreatic Elastase / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D013552:Swine; D014357:Trypsin / Q000494:pharmacology*
D007457:Iodine Radioisotopes; D004549:Elastin; D010196:Pancreatic Elastase; D014357:Trypsin
Amélioration des conditions techniques de l'evaluation d'une activité élastolytique. Role activateur de la trypsine