title: The genome of infectious bursal disease virus consists of two segments of double-stranded RNA.
abstract: The RNA of infectious bursal disease virus was reexamined in a detailed analysis. It could be established that its genome consists of two segments of double-stranded RNA. The RNA is RNase resistant and has a sedimentation coefficient of 14S and a buoyant density of 1.62 g/ml. The purine/pyrimidine ratio is nearly 1; the guanine plus cytosine content is 55.3%; the Tm is 95.5 degrees C. The molecular weights of the two double-stranded segments were determined to be 2.2 x 10(6) and 2.5 x 10(6).
mesh_terms: D002499:Centrifugation, Density Gradient; D007243:Infectious bursal disease virus / Q000032:analysis*; D008970:Molecular Weight; D009711:Nucleotides / Q000032:analysis; D012330:RNA, Double-Stranded / Q000032:analysis*; D012367:RNA, Viral / Q000032:analysis*; D012087:Reoviridae / Q000032:analysis*; D012260:Ribonucleases / Q000378:metabolism
chemical_list: D009711:Nucleotides; D012330:RNA, Double-Stranded; D012367:RNA, Viral; D012260:Ribonucleases