18-Hydroxylation of deoxycorticosterone by reconstituted systems from rat and bovine adrenals.
18-Hydroxylation of deoxycorticosterone was studies with rat or bovine adrenal mitochondria or with reconstituted systems obtained from these fractions. The reconstituted systems consisted of a partially purified preparation of cytochrome P-450 from rat adrenals and a partially purified NADPH-cytochrome P450 reductase preparation from bovine adrenals. In some experimenta a soluble cytochrome P-450 fraction from bovine adrenals was used. Adrenodoxine and adrenodoxine reductase were shown to be the active components of the NADPH-cytochrome P-450 reductase preparation. Optimal assay conditions were determined for 18-hydroxylation by the crude mitochondrial fraction as well as by the reconstituted systems. In the presence of excess NADPH-cytochrome P-450 reductase fraction, the rate of 18-hydroxylation was linear with time and with the amount of cytochrome P-450. In incubations with intact rat adrenal mitochondria to which Ca2+ and an excess NADPH had been added, NADPH-cytochrome P-450 reductase increased the rate of 18-hydroxylation about 100%, indicating that NADPH-cytochrome P-45o reductase was to some extent rate-limiting. The rate of 18-hydroxylation of deoxycorticosterone by the reconstituted system as well as by intact mitochondrial fraction was much higher than the rat of 18-hydroxylation of corticosterone and progesterone. When the cytochrome P-450 preparation from rat adrenals in the reconstituted system was substituted for cytochrome P-450 from bovine adrenals, the rate of 18-hydroxylation decreased considerably. Under all experimental conditions, the 18-hydroxylation of deoxycorticosterone occurred with a concomitant and efficient 11beta-hydroxylation. Provided the source of cytochrome P-450 was the same, the ratio between 11beta- and 18hydroxylation was constant under all conditions and was not significantly different in the presence of metopirone, carbon monoxide, cytochrome c or different steroids. It is suggested that identical or at least very similar types of cytochrome P-450 are involved in 11beta- and 18-hydroxylation of deoxycorticosterone.
D000311:Adrenal Glands / Q000378:metabolism*; D000325:Adrenodoxin / Q000494:pharmacology; D000818:Animals; D002417:Cattle; D003577:Cytochrome P-450 Enzyme System / Q000494:pharmacology; D003579:Cytochrome Reductases / Q000494:pharmacology; D003900:Desoxycorticosterone / Q000378:metabolism*; D005287:Ferredoxin-NADP Reductase / Q000494:pharmacology; D006900:Hydroxylation; D008297:Male; D008928:Mitochondria / Q000378:metabolism; D009249:NADP / Q000494:pharmacology; D051381:Rats; D013045:Species Specificity; D013256:Steroids / Q000494:pharmacology
D013256:Steroids; D000325:Adrenodoxin; D003900:Desoxycorticosterone; D009249:NADP; D003577:Cytochrome P-450 Enzyme System; D005287:Ferredoxin-NADP Reductase; D003579:Cytochrome Reductases