Use of mini-columns packed with acid-washed Florisil for the rapid separation of A, E and F series prostaglandins.
A new procedure was developed for the rapid isolation of the A, E and F series prostaglandins from plasma extracts. This new procedure involves the use of mini-columns packed with acid-washed Florisil to rapidly separate the A, E and F series prostaglandins. These new mini-columns give flow rates of around 1 ml/min. and they generate a well-resolved chromatographic pattern while at the same time producing good recoveries for [3H]-labeled prostaglandins of the A, E and F series. Using these new mini-columns, large numbers of plasma extracts can be processed in a short period of time.
D002845:Chromatography / Q000379:methods*; D005069:Evaluation Studies as Topic; D006801:Humans; D008274:Magnesium; D011453:Prostaglandins / Q000302:isolation & purification*; D011454:Prostaglandins A / Q000302:isolation & purification; D011458:Prostaglandins E / Q000302:isolation & purification; D011460:Prostaglandins F / Q000302:isolation & purification; D012824:Silicic Acid; D012822:Silicon Dioxide
D011453:Prostaglandins; D011454:Prostaglandins A; D011458:Prostaglandins E; D011460:Prostaglandins F; D012824:Silicic Acid; D012822:Silicon Dioxide; D008274:Magnesium