Improved in vitro light activation and assay systems for two spinach chloroplast enzymes.
An improved system for the in vitro light activation of the chloroplast enzymes fructose-1,6-bisphosphatase and NADP-dependent malate dehydrogenase is described. Through the presence of the monothiol beta-mercaptoethanol in the reaction mixtures the activated forms of the enzymes can be stabilized and their activity determined spectrophotometrically.
D002736:Chloroplasts / Q000201:enzymology*; D004789:Enzyme Activation / Q000528:radiation effects; D006597:Fructose-Bisphosphatase / Q000378:metabolism*; D008027:Light; D008291:Malate Dehydrogenase / Q000378:metabolism*; D008623:Mercaptoethanol / Q000494:pharmacology; D010944:Plants / Q000201:enzymology
D008623:Mercaptoethanol; D008291:Malate Dehydrogenase; D006597:Fructose-Bisphosphatase