title: [Covalent bond between RNA and protein in encephalomyocarditis virus].
abstract: Deproteinized encephalomyocarditis [32P]RNA, after digestion with a mixture of RNases A, T1 and T2, yields mononucleotides and a labelled compound, which is positively charged at pH 3.5. This product can be digested with pronase and has a close electrophoretic mobility to a protein with a molecular weight of 7000-8000. Covalently bound nucleotide-peptides were isolated from this compound after treatment with RNases and pronase. It was shown that the 5'-terminal uridylic acid is covalently linked with peptides. The phosphodiester bond between uridylic acid and peptides is discussed.
mesh_terms: D004686:Encephalomyocarditis virus / Q000032:analysis*; D008970:Molecular Weight; D011402:Pronase; D011485:Protein Binding; D012367:RNA, Viral; D012260:Ribonucleases; D014764:Viral Proteins
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins; D012260:Ribonucleases; D011402:Pronase
vernacular_title: Kovalentnoe soedinenie RNK i belka v viruse zntsefalomnokardita.