Isolation of cytopathogenic Rotavirus from neonatal calves.
Rotavirus was isolated in rolled calf kidney cultures from the intestinal contents of a calf suffering from diarrhoea. The cytopathic effect was demonstrated in native cultures as well as in stained preparations. The affected cells were elongated, became sickle-like in shape, disintegrated and detached from the monolayer. Virus-specific direct immunofluorescence ran parallel, in both time and intensity, with the cytopathic effects. Immuno-electron microscopy showed aggregated virus particles corresponding in size, shape and structure to Rotavirus virion.
D000818:Animals; D000831:Animals, Newborn / Q000382:microbiology*; D000956:Antigens, Viral / Q000032:analysis; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D002478:Cells, Cultured; D003588:Cytopathogenic Effect, Viral; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary*; D012328:RNA Viruses / Q000302:isolation & purification*; D012401:Rotavirus / Q000302:isolation & purification* / Q000502:physiology / Q000648:ultrastructure; D014777:Virus Diseases / Q000382:microbiology / Q000662:veterinary*
D000956:Antigens, Viral