[The influence of clanobutin on bile secretion in the isolated perfused rat liver (author's transl)].
Isolated perfused rat liver was used to characterize the choleretic action of 4-[4-chloro-N-(4-methoxyphenyl)-benzamido]-butyric acid (clanobutin). The dose-dependent increase of the bile flow was associated with an increase of the Na+ secretion. In in vitro studies the drug inhibited the Na+-K+-ATPase of the sinusoidal membrane more than that of the canalicular membrane in a dose-dependent manner. The following hypothesis was proposed to explain the choleretic action of clanobutin. The intracellular Na+ concentration increased due to the inhibition of the sinusoidal Na+-K+-ATPase. This is then compensated by transporting the extra Na+ into the bile. However, it cannot be ruled out that Na+ is transported as counterion during hepatobiliary transport of clanobutin or else its metabolites. The effect of the drug seems to be non-specific since other enzymes of the plasma membrane were also inhibited.
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001549:Benzamides / Q000494:pharmacology; D001646:Bile / Q000378:metabolism*; D066298:In Vitro Techniques; D007931:Leucyl Aminopeptidase / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D010042:Ouabain / Q000494:pharmacology; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013997:Time Factors; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology*
D001549:Benzamides; D005680:gamma-Aminobutyric Acid; D010042:Ouabain; D000469:Alkaline Phosphatase; D007931:Leucyl Aminopeptidase; D000251:Adenosine Triphosphatases; D000254:Sodium-Potassium-Exchanging ATPase
Der Einfluss von Clanobutin auf die Gallesekretion der isoliert perfundierten Rattenleber.