A blastogenic test for foot-and-mouth disease.
A blastogenic test to detect peripheral blood leukocytes specifically sensitized to foot-and-mouth disease virus antigen is described. The test is carried out in microtitre plates and optimum conditions were found by titration. These employed 7.5 x 10(5) cells/well and 20 complement fixing units of antigen. Peak [3H]thymidine incorporation was found to take place at 2-3 days.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D005537:Aphthovirus / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology* / Q000517:prevention & control; D005536:Foot-and-Mouth Disease / Q000276:immunology* / Q000517:prevention & control; D007111:Immunity, Cellular; D008213:Lymphocyte Activation; D008297:Male; D014765:Viral Vaccines / Q000276:immunology
D000956:Antigens, Viral; D014765:Viral Vaccines