Monocyte and granulocyte defect in chronic lymphocytic leukemia.
Enzymatically homogeneous populations of lymphocytes, monocytes, and neutrophils were isolated by zonal centrifugation from 5 untreated patients with chronic lymphocytic leukemia (CLL) and 2 patients with CLL in full remission. The cells were then quantitatively analyzed for six leukocytic enzymes and compared with cells from normal subjects. CLL monocytes were deficient in beta-glucuronidase (0.06 units; normal, 0.16), myeloperoxidase (0.07 mg; normal, 0.5 mg), and lysozyme (0.7 mg; normal, 3.3 mg). In 2 cases, CLL neutrophils were severely deficient in lysozyme (1 to 2 mg; normal, 7 mg) and myeloperoxidase (2 to 3 mg; normal, 7 mg). Neutrophil alkaline phosphatase and neutral protease were unaffected. CLL lymphocytes shared with the monocytes the deficiency of beta-glucuronidase (0.03 units; normal, 0.09 units). The 2 CLL patients in full remission carried normal enzyme levels in leukocytes of all three cell lines. The CLL lymphocytes of untreated patients were unresponsive to mitogens but became responsive in remission. The CLL monocytes from both untreated and treated patients transformed into macrophages. The pattern of shared enzyme deficiency among lymphocytes, monocytes, and neutrophils of CLL patients and its normalization in all three cell types under remission suggest that the differentiation of the three leukocytic cell lines may be an enzymatically interlinked process and that the deficiency of these enzymes in leukemia may reflect an interrelated aberrant differentiation of the leukemic cells.
D000135:Acid Phosphatase / Q000097:blood; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D005966:Glucuronidase / Q000097:blood; D006098:Granulocytes / Q000201:enzymology*; D006801:Humans; D007945:Leukemia, Lymphoid / Q000201:enzymology*; D009000:Monocytes / Q000201:enzymology*; D009113:Muramidase / Q000097:blood; D009195:Peroxidase / Q000097:blood
D009195:Peroxidase; D000135:Acid Phosphatase; D009113:Muramidase; D005966:Glucuronidase