Lipoproteins in plasma from patients with low LCAT activity due to biliary obstruction.
A high density lipoprotein (HDL) fraction, designated cholestatic HDL1, has been isolated by zonal ultracentrifugation from patients with long-standing biliary obstruction. This lipoprotein fraction consists exclusively of disc-shaped particles rich in phospholipid and unesterified cholesterol. The fatty acid composition of the minor cholesteryl ester fraction was characterized by a low content of linoleic acid. Apoprotein E was the dominating protein' component, while only small amounts of apolipoprotein A-I were found. Immunochemical studies of cholestatic HDL1 indicate that a considerable proportion of the particles contained apo E as its only protein constituent. All patients had a markedly reduced endogenous cholesterol-esterifying ability. The finding of apparently primary LpE particles in biliary obstruction may be related to this secondary LCAT deficiency.
D001059:Apoproteins / Q000097:blood; D001798:Blood Proteins / Q000032:analysis; D002779:Cholestasis / Q000097:blood* / Q000201:enzymology; D002784:Cholesterol / Q000097:blood; D002788:Cholesterol Esters / Q000097:blood; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D008075:Lipoproteins, HDL / Q000032:analysis / Q000097:blood*; D007862:Phosphatidylcholine-Sterol O-Acyltransferase / Q000097:blood*; D010743:Phospholipids / Q000097:blood; D014280:Triglycerides / Q000097:blood
D001059:Apoproteins; D001798:Blood Proteins; D002788:Cholesterol Esters; D008075:Lipoproteins, HDL; D010743:Phospholipids; D014280:Triglycerides; D002784:Cholesterol; D007862:Phosphatidylcholine-Sterol O-Acyltransferase