Method for measurement of human urinary kininase activity.
A method was developed to measure kininase activity in human urine. The method consists of dialysis of human centrifuged urine sample against phosphate buffer and partial fractionation of A-50 Sephadex column. The enzymatic property of urinary kininase, which destroys bradykinin when incubated, is estimated from its effect on a definite amount of bradykinin, using rat uterus.
D000818:Animals; D001681:Biological Assay; D001920:Bradykinin / Q000494:pharmacology; D005260:Female; D006801:Humans; D007700:Kinetics; D007703:Peptidyl-Dipeptidase A / Q000652:urine*; D051381:Rats; D014599:Uterus / Q000187:drug effects
D007703:Peptidyl-Dipeptidase A; D001920:Bradykinin