Quantitative magnetic resonance studies of manganese uptake by mitochondria.
The uptake of the paramagnetic ion manganese by rat liver mitochondria is studied by electron paramagnetic resonance (EPR) spectroscopy. Emphasis is placed on: (a) obtaining accurate EPR quantitation of intramitochondrial manganese fractions previously described (Gunter, T. E., and J. S.Puskin, 1972, Biophys. J. 12:625) (b) establishing competition for intramitochondrial binding between one of these fractions and calcium, (c) demonstrating the effects of substrate and ATP concentrations on each fraction observed through EPR, and (d) demonstrating the effect of inorganic phosphate (Pi) concentration and pH on each fraction.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D001665:Binding Sites; D001692:Biological Transport; D002118:Calcium / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D006863:Hydrogen-Ion Concentration; D008345:Manganese / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D009840:Oligomycins / Q000494:pharmacology; D010710:Phosphates / Q000378:metabolism; D051381:Rats; D013054:Spectrophotometry, Atomic; D013386:Succinates / Q000494:pharmacology
D009840:Oligomycins; D010710:Phosphates; D013386:Succinates; D008345:Manganese; D000255:Adenosine Triphosphate; D002118:Calcium