Foot-and-mouth disease virus immunogenic capsid protein VPT: N-terminal sequences and immunogenic peptides obtained by CNBr and tryptic cleavages.
The immunogenic capsid protein (VPT), circa 30 kiladaltons (kd), of foot-and-mouth disease virus was examined for (i) its ability to induce neutralizing antibody in guinea pigs after chemical modifications and CNBr or tryptic cleavages and (ii) N-terminal amino sequence homology across three virus types. The immunogenicity of VPT was inactivated by glutaraldehyde treatment, carboxymethylation and maleylation or citraconylation. However, de-citraconylation restored part of the lost activity. Cleavage of type A12 VPT with CNBr produced an immunogenic peptide of circa 13 kd. A slightly larger (ca. 16 kd) immunogenic doublet, VPTab, was obtained by tyrptic cleavage of VPT in the virion. Sequence homologies of circa 85% were found between the first 26 amino acids at the N-terminus of VP chains from virus types A12 strain 119 (A12), C3 Resende (C3R) and O1 Brugge (O1B).
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis*; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D005537:Aphthovirus / Q000032:analysis* / Q000187:drug effects / Q000276:immunology; D002213:Capsid / Q000032:analysis* / Q000276:immunology; D003488:Cyanogen Bromide / Q000494:pharmacology; D006168:Guinea Pigs; D008970:Molecular Weight; D009500:Neutralization Tests; D010455:Peptides / Q000032:analysis* / Q000276:immunology; D014357:Trypsin / Q000494:pharmacology; D014764:Viral Proteins / Q000032:analysis*
D000596:Amino Acids; D000914:Antibodies, Viral; D010455:Peptides; D014764:Viral Proteins; D014357:Trypsin; D003488:Cyanogen Bromide