Glycosaminoglycan-lipoprotein complexes from aortas of hypercholesterolemic rabbits. Part 1. Isolation and characterization.
Glycosaminoglycan-lipoprotein complexes were isolated from rabbit aortas exhibiting nearly confluent cholesterol-induced foam cell lesions by extraction with 0.15 M NaCl. Purification and characterization was achieved by gel chromatography, non-ionic differential flotation and by cellulose polyacetate electrophoresis. Analysis showed that these complexes consisted of very low density lipoproteins, heparan sulfate, chondroitin sulfate-C and hyaluronic acid. The demonstration that rabbit intimal foam cell lesions contain  extractable glycosaminoglycan-lipoprotein complexes makes this animal model an excellent tool for further studies on the role of these complexes in the atherogenic process.
D000818:Animals; D001011:Aorta / Q000032:analysis*; D001161:Arteriosclerosis; D004195:Disease Models, Animal; D006025:Glycosaminoglycans / Q000302:isolation & purification*; D006937:Hypercholesterolemia / Q000473:pathology*; D008074:Lipoproteins / Q000302:isolation & purification*; D008077:Lipoproteins, LDL / Q000302:isolation & purification; D008079:Lipoproteins, VLDL / Q000302:isolation & purification; D011817:Rabbits
D006025:Glycosaminoglycans; D008074:Lipoproteins; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL