Polymorphonuclear leukocyte phagocytosis: quantitation by a rapid radioactive method.
A rapid quantitative assay of phagocytosis has been developed. The method has been used to determine the phagocytic capability of human or dog polymorphonuclear leukocytes (PMN's) in a defined medium without added sera. The engulfment of 3H-labeled Staphylococcus albus is essentially complete in 30 minutes at 37 degrees C. Increase of phagocyte radioactivity is directly proportional to PMN per cubic centimeter over a wide range of cell concentrations. Maximal phagocytosis is observed at pH 7.5.
D000818:Animals; D003470:Culture Media; D004285:Dogs; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007158:Immunologic Techniques; D066298:In Vitro Techniques; D007962:Leukocytes / Q000276:immunology*; D010587:Phagocytosis; D013210:Staphylococcus / Q000276:immunology; D013997:Time Factors; D014316:Tritium
D003470:Culture Media; D014316:Tritium