Metabolism of red blood cells in chronic renal failure. I. Glycolytic enzyme levels.
This paper starts a series on red blood cell (RBC) metabolism in patients with chronic renal failure (CRF). The glycolytic enzyme levels and in vitro half-lives of these patients' RBCs were determined. A number of enzymes (hexokinase, glucose-6-phosphate isomerase, fructose-6-phosphate kinase, aldolase, glyceraldehyde-3-phosphate dehydrogenase and lactate dehydrogenase) showed higher activities than in normal control RBCs. Other enzyme activities were normal. These results were discussed and several possible mechanisms considered. We favour the point of view of a shortened life span of the RBCs in CRF, making the most unstable enzymes of the glycolytic sequence appear increase as compared with normal controls.
D000293:Adolescent; D000328:Adult; D004912:Erythrocytes / Q000201:enzymology*; D005260:Female; D005634:Fructose-Bisphosphate Aldolase / Q000097:blood; D005636:Fructosephosphates / Q000097:blood; D005956:Glucose-6-Phosphate Isomerase / Q000097:blood; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000097:blood; D006593:Hexokinase / Q000097:blood; D006801:Humans; D007676:Kidney Failure, Chronic / Q000201:enzymology* / Q000628:therapy; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008297:Male; D008875:Middle Aged; D010732:Phosphofructokinase-1 / Q000097:blood; D010735:Phosphoglycerate Kinase / Q000097:blood; D010736:Phosphoglycerate Mutase / Q000097:blood; D010751:Phosphopyruvate Hydratase / Q000097:blood; D011770:Pyruvate Kinase / Q000097:blood; D006435:Renal Dialysis; D014305:Triose-Phosphate Isomerase / Q000097:blood
D005636:Fructosephosphates; D007770:L-Lactate Dehydrogenase; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D006593:Hexokinase; D010732:Phosphofructokinase-1; D011770:Pyruvate Kinase; D010735:Phosphoglycerate Kinase; D005634:Fructose-Bisphosphate Aldolase; D010751:Phosphopyruvate Hydratase; D014305:Triose-Phosphate Isomerase; D005956:Glucose-6-Phosphate Isomerase; D010736:Phosphoglycerate Mutase