title: [Denaturation changes in the proteins of the membrane structures of the brain during deprivation of the paradoxical stage of sleep].
abstract: 24--96-hour REM-sleep deprivation entailed a rise of SH-group content in the proteins of the rat diencephalon and mesencephalon tissue homogenates, whereas SH-group content of the homogenates of the cortex, pons with medulla oblongata, and cerebellum remained unchanged. Neither did the content of SH-groups change in the soluble protein fraction nor the content of low-molecular thiol substances in the brain parts under study. From among the subcellular fractions of the brain-stem homogenate, the increase of SH-group content after 24-hour REM-sleep deprivation occurred in the synaptosomal and nuclear fractions but not in the mitochondrial one. The REM-sleep deprivation did not affect the --S--S-group content in these fractions. In the anterior parts of the brain-stem only REM-sleep deprivation led to conformational changes of structural proteins towards their denaturation but without rupture of the disulfide bonds.
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D001933:Brain Stem / Q000032:analysis; D002531:Cerebellum / Q000032:analysis; D002540:Cerebral Cortex / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D005260:Female; D005625:Frontal Lobe / Q000032:analysis; D008297:Male; D009419:Nerve Tissue Proteins / Q000032:analysis*; D009778:Occipital Lobe / Q000032:analysis; D011149:Pons / Q000032:analysis; D011489:Protein Denaturation; D051381:Rats; D012892:Sleep Deprivation; D012895:Sleep, REM / Q000502:physiology*; D013997:Time Factors
chemical_list: D009419:Nerve Tissue Proteins
vernacular_title: Denaturatsionnye sdvigi belkov membrannykh struktur golovnogo mozga pri lishenii paradoksal'noĭ fazy sna.