Nuclear and cytoplasmic deoxyribonucleic acid polymerases from rat nephroma cells.
We have examined the DNA polymerases found in a rat nephroma cell line. Using DEAE- and DNA-cellulose chromatography, we have found two major cytoplasmic DNA polymerases and one major and three minor DNA polymerases from the nucleus. The enzymes were all purified, characterized, and distinguished from each other by several criteria. The enzyme require, for maximal activity, a natural or synthetic double-stranded DNA, four deoxynucleoside, triphosphates, and magnesium. They are inhibited to varying degrees by sodium pyrophosphate, ethidium bromide, and rho-chloromercuribenzoate.
D000818:Animals; D002460:Cell Line; D002467:Cell Nucleus / Q000201:enzymology*; D002729:Chloromercuribenzoates / Q000494:pharmacology; D002846:Chromatography, Affinity; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D003593:Cytoplasm / Q000201:enzymology*; D004254:DNA Nucleotidyltransferases / Q000302:isolation & purification / Q000378:metabolism*; D004279:DNA, Viral / Q000096:biosynthesis; D011756:Diphosphates / Q000494:pharmacology; D004720:Endonucleases / Q000378:metabolism; D004996:Ethidium / Q000494:pharmacology; D005092:Exonucleases / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D008970:Molecular Weight; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats; D012965:Sodium Chloride / Q000494:pharmacology; D013698:Templates, Genetic; D013936:Thymidine / Q000378:metabolism; D014316:Tritium; D009396:Wilms Tumor / Q000201:enzymology*
D002729:Chloromercuribenzoates; D004279:DNA, Viral; D011756:Diphosphates; D014316:Tritium; D012965:Sodium Chloride; D011189:Potassium Chloride; D004254:DNA Nucleotidyltransferases; D004720:Endonucleases; D005092:Exonucleases; D004996:Ethidium; D008274:Magnesium; D013936:Thymidine