[Usage of the gel filtration method for the determination of urinary proteins (author's transl)].
The urinary proteins determination was carried out after purification by gel filtration over Sephadex rosin. The proteins eluaded react with Biuret-reagent to establish the coloured copper-proteins complex. If proteins concentrations is inferior, the proteins can be determinated by the copper-proteins complex isolation with second gel filtration and titrating Cu++ with reactives: dietildithiocarbamate or the PAR (4-(2pyridyl-azo)resorcinol monosodium salt).
D001737:Biuret; D002850:Chromatography, Gel / Q000379:methods; D003300:Copper; D004050:Ditiocarb; D006801:Humans; D011507:Proteinuria / Q000652:urine*; D012118:Resorcinols; D013057:Spectrum Analysis
D012118:Resorcinols; D003300:Copper; D001737:Biuret; D004050:Ditiocarb; C028398:4-(2-pyridylazo)resorcinol
Utilizzazione della gel filtration per la determinazione della proteinuria.