Influences of verapamil, X-537A, A-23187 and adenosine 3',5'-cyclic monophosphate on release of 5-hydroxytryptamine from rat brain slices.
Influences of verapamil, X-537A, A-23187 and cyclic-AMP on the release of [3H]-5-HT taken up into rat brain slices, were examined. Incubation with 40 mM KCl induced tritium release which was dependent on the presence of Ca2+. Verapamil, which blocks Ca2+ influx in excitable tissues, decreased potassium-induced release of 5-HT. Tritium release induced by ionophore X-537A was not dependent on extracellular Ca2+ while that induced by A-23187 required Ca2+. Cyclic-AMP, dibutyryl cyclic-AMP and theophylline did not significantly stimulate 5-HT release either in the presence or absence of Ca2+.
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001921:Brain / Q000378:metabolism*; D000001:Calcimycin / Q000494:pharmacology*; D002118:Calcium / Q000502:physiology; D000242:Cyclic AMP / Q000494:pharmacology*; D066298:In Vitro Techniques; D007832:Lasalocid / Q000494:pharmacology*; D008297:Male; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D012701:Serotonin / Q000378:metabolism*; D013806:Theophylline / Q000494:pharmacology; D014700:Verapamil / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D012701:Serotonin; D000001:Calcimycin; D013806:Theophylline; D014700:Verapamil; D000242:Cyclic AMP; D011188:Potassium; D002118:Calcium; D007832:Lasalocid