Evidence that the avian sarcoma virus transforming gene product is a cyclic AMP-independent protein kinase.
The avian sarcoma virus transforming gene product pp60src has been partially purified by using ion exchange or immunoaffinity chromatography. These preparations contain a cyclic AMP-independent protein kinase activity capable of transferring radiolabel from [gamma-32P]ATP to immune rabbit IgG, casein, and the heavy chain purified from normal rabbit IgG. The casein kinase activity is specifically inhibited by anti-pp60src IgG. Comparison of the pp60src-protein kinase isolated from cells transformed by a wild-type ASV or by an ASV temperature-sensitive transformation mutant revealed that the latter product had a half-life of 3 min at 41 degrees C whereas that of the wild-type product was 20 min.
D000818:Animals; D001354:Avian Leukosis Virus / Q000235:genetics*; D002472:Cell Transformation, Viral; D000242:Cyclic AMP / Q000378:metabolism; D007074:Immunoglobulin G; D009154:Mutation; D011494:Protein Kinases / Q000235:genetics* / Q000302:isolation & purification; D012513:Sarcoma, Experimental / Q000201:enzymology*; D013696:Temperature; D014764:Viral Proteins / Q000378:metabolism*
D007074:Immunoglobulin G; D014764:Viral Proteins; D000242:Cyclic AMP; D011494:Protein Kinases