Release of corticotrophin releasing factor and other hypophysiotropic substances from isolated nerve-endings (synaptosomes).
Synaptosomes isolated from the sheep and the rat hypothalamus contain corticotrophin releasing factor, vasopressin, prolactin-release inhibiting factor and probably all of the substances which participate in the regulation of adenohypophysial function. Electrical field stimulation or depolarizing concentrations (55 mmol/l) of potassium ions cause a release of these factors from the incubated nerve-endings in a calcium-dependent manner. It is suggested that synaptosomes may provide a valuable approach to the study of mechanisms involved in hypothalamic neurosecretion in vitro.
D000324:Adrenocorticotropic Hormone / Q000378:metabolism; D000818:Animals; D001681:Biological Assay; D002118:Calcium / Q000494:pharmacology; D003346:Corticotropin-Releasing Hormone / Q000378:metabolism*; D003470:Culture Media; D004558:Electric Stimulation; D005260:Female; D007031:Hypothalamus / Q000378:metabolism* / Q000648:ultrastructure; D066298:In Vitro Techniques; D007773:Lactates / Q000378:metabolism; D008297:Male; D010101:Oxygen Consumption; D011188:Potassium / Q000494:pharmacology; D011388:Prolactin / Q000378:metabolism; D011389:Prolactin Release-Inhibiting Factors / Q000378:metabolism*; D011863:Radioimmunoassay; D051381:Rats; D012756:Sheep; D013347:Subcellular Fractions / Q000378:metabolism; D013574:Synaptosomes / Q000378:metabolism*; D014667:Vasopressins / Q000378:metabolism*
D003470:Culture Media; D007773:Lactates; D014667:Vasopressins; D000324:Adrenocorticotropic Hormone; D011388:Prolactin; D003346:Corticotropin-Releasing Hormone; D011389:Prolactin Release-Inhibiting Factors; D011188:Potassium; D002118:Calcium