title: Intracellular cyclic GMP-binding proteins in cellular slime molds.
abstract: The complexity of cyclic GMP-binding activity in the 48,000 X g supernatant of three species of the cellular slime molds (Dictyostelium discoideum, Dictyostelium rosarium, and Polysphondylium violaceum) was studied by gel filtration chromatography on AcA 34 Ultrogel. All these species have in common a cyclic GMP-binding protein of molecular weight of about 2.5 X 10(5) which specifically binds this nucleotide. In addition, Scatchard plots of assays carried out with the 48,000 X g supernatant of these species exhibit cyclic GMP-binding activity with an apparent dissociation constant of about 1 nM. None of the cyclic GMP-binding proteins separated by chromatography on AcA 34 Ultrogel was associated to protein kinase activity stimulation. In view of the cyclic GMP function during chemotactic transduction in the cellular slime molds, the possible molecular function for this 2.5 X 10(5)-dalton cyclic GMP-binding protein is discussed.
mesh_terms: D002352:Carrier Proteins / Q000032:analysis* / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism*; D004023:Dictyostelium / Q000032:analysis*; D005656:Fungal Proteins / Q000032:analysis* / Q000378:metabolism; D009235:Myxomycetes / Q000032:analysis*; D011494:Protein Kinases / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D005656:Fungal Proteins; D011494:Protein Kinases; D006152:Cyclic GMP