Inactivation of membrane-bound (Na+ +K+)-ATPase of Yoshida sarcoma cells and cobra venom cytotoxin complex with the glycolipid components of the enzyme system.
The molecular mechanisms involved in the inactivation of (Na+ + K+)-stimulated ATPase of Yoshida sarcoma cells by a cytotoxic protein (P6) from cobra venom have been examined. The overall data obtained using purified (Na+ + K+)-stimulated ATPase of Yoshida sarcoma cells suggest that cytotoxin P6 combines with phosphatidyl serine and a glycolipid which are closely associated with (Na+ + K+)-stimulated ATPase which in turn may lead to the inactivation of the enzyme in this cell system.
D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002462:Cell Membrane / Q000201:enzymology; D004546:Elapid Venoms / Q000494:pharmacology*; D006017:Glycolipids / Q000502:physiology*; D007700:Kinetics; D008563:Membrane Lipids / Q000502:physiology; D051379:Mice; D010718:Phosphatidylserines / Q000502:physiology; D012517:Sarcoma, Yoshida / Q000201:enzymology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
D002241:Carbohydrates; D004546:Elapid Venoms; D006017:Glycolipids; D008563:Membrane Lipids; D010718:Phosphatidylserines; D000254:Sodium-Potassium-Exchanging ATPase