Human brain in tissue culture. I. Acquisition, initial processing, and establishment of brain cell cultures.
This paper details the in vitro techniques used to establish cells in culture from the brains of 40 patients, most of whom had chronic neurologic disease. The clinical and pathologic features of these patients are given. The success in establihsing cell lines was dependent upon the origin of tissue (biopsy vs. autopsy), the site of removal from the brain, and various environmental and technical manipulations in vitro.
D000293:Adolescent; D000328:Adult; D001344:Autopsy; D001369:Axons / Q000166:cytology; D001706:Biopsy / Q000009:adverse effects; D001921:Brain / Q000166:cytology* / Q000601:surgery; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D002478:Cells, Cultured; D002831:Choroid Plexus / Q000166:cytology; D003470:Culture Media; D005260:Female; D005615:Freezing; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D008722:Methods; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000473:pathology; D009413:Nerve Fibers, Myelinated / Q000166:cytology; D009900:Optic Nerve / Q000166:cytology; D013997:Time Factors; D014357:Trypsin / Q000494:pharmacology
D003470:Culture Media; D014357:Trypsin