Interferon susceptibility of various cell lines persistently infected with haemagglutinating virus of Japan (HVJ).
Various cell lines persistently infected with para-influenza 1 virus, HVJ strain, were less susceptible to the antiviral action of interferon than the same cell lines when not infected with HVJ. When Vero cells persistently infected with a temperature-sensitive strain of HVJ were incubated at 38 degrees C, a non-permissive temperature, they became fully susceptible to interferon, whereas neither the haemadsorbing nor the cell-associated haemagglutinating activity of the virus was expressed. These findings suggest that the lowered interferon susceptibility of virus-carrier cells may be related to the maturation of virus in them. It was found that the low susceptibility of virus-carrier cells to interferon is not due to blocked adsorption of interferon or to inability of the cells to respond tointerferon. Studies with actinomycin D suggest that some step (or steps) before the synthesis of the messenger RNA for the antiviral protein is blocked.
D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D006224:Cricetinae; D003609:Dactinomycin / Q000494:pharmacology; D000882:Haplorhini; D007372:Interferons / Q000494:pharmacology*; D007668:Kidney; D008251:Macaca; D010222:Parainfluenza Virus 1, Human / Q000254:growth & development*; D013696:Temperature; D014779:Virus Replication / Q000187:drug effects
D003609:Dactinomycin; D007372:Interferons