[Reconstitution of electrogenic function of pyrophosphatase isolated from Rhodospirillum rubrum membranes].
The membrane vesicles (proteoliposomes) have been reconstituted from phospholipids and inorganic pyrophosphatase (EC 3.6.1.1) isolated from Rhodospirillum rubrum chromatophores. An addition of inorganic pyrophosphate (PPi) causes a Mg2+-dependent formation of a transmembrane electric potential difference and an uptake of penetrating tetraphenylborate anions by the proteoliposomes. Thus, isolated pyrophosphatase, being incorporated into the phospholipid membrane, functions as a MgPPi-dependent protein generator of the electric current.
D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D007700:Kinetics; D008081:Liposomes; D008274:Magnesium / Q000494:pharmacology; D008564:Membrane Potentials; D011510:Proteolipids; D011755:Pyrophosphatases / Q000378:metabolism*; D012247:Rhodospirillum rubrum / Q000201:enzymology*
D008081:Liposomes; D011510:Proteolipids; D011755:Pyrophosphatases; D008274:Magnesium
Rekonstruktsiia elektrogennoĭ funktsii izolirovannoĭ membrannoĭ pirofosfatazy Rhodospirillum Rubrum.