title: Enzymes of pyrimidine metabolism in Mycoplasma mycoides subsp. mycoides.
abstract: The major pathways of ribonucleotide biosynthesis in Mycoplasma mycoides subsp. mycoides have been proposed from studies on its use of radioactive purines and pyrimidines. To interpret more fully the observed pattern of pyrimidine usage, cell extracts of this organism have been assayed for several enzymes associated with the salvage synthesis of pyrimidine nucleotides. M. mycoides possessed uracil phosphoribosyltransferase, uridine phosphorylase, uridine (cytidine) kinase, uridine 5'-monophosphate kinase, and cytidine 5'-triphosphate synthetase. No activity for phosphorolysis of cytidine was detected, and no in vitro conditions were found to give measurable deamination of cytidine. Of the two potential pathways for incorporation of uridine, our data suggest that this precursor would largely undergo initial phosphorolysis to uracil and ribose-1-phosphate. Conversely, cytidine is phosphorylated directly to cytidine 5'-monophosphate in its major utilization, although conversion of cytidine to uracil, uridine, and uridine nucleotide has been observed in vivo, at least when uracil is provided in the growth medium. Measurements of intracellular nucleotide contents and their changes on additions of pyrimidine precursors have allowed suggestions as to the operation of regulatory mechanisms on pyrimidine nucleotide biosynthesis in M. mycoides in vivo. With uracil alone or uracil plus uridine as precursors of pyrimidine ribonucleotides, the regulation of uracil phosphoribosyltransferase and cytidine 5'-triphosphate synthetase is probably most important in determining the rate of pyrimidine nucleotide synthesis. When cytidine supplements uracil in the growth medium, control of cytidine kinase activity would also be important in this regard.
mesh_terms: D002474:Cell-Free System; D003564:Cytidine Deaminase / Q000378:metabolism; D003570:Cytidine Triphosphate; D008025:Ligases / Q000378:metabolism; D009176:Mycoplasma mycoides / Q000201:enzymology* / Q000378:metabolism; D009703:Nucleoside-Phosphate Kinase / Q000378:metabolism; D010430:Pentosyltransferases / Q000378:metabolism; D011743:Pyrimidines / Q000378:metabolism*; D013379:Substrate Specificity; D014541:Uridine Kinase / Q000378:metabolism; D014542:Uridine Monophosphate; D014543:Uridine Phosphorylase / Q000378:metabolism; D014544:Uridine Triphosphate
chemical_list: D011743:Pyrimidines; D003570:Cytidine Triphosphate; D014542:Uridine Monophosphate; D010430:Pentosyltransferases; D014543:Uridine Phosphorylase; C023269:uracil phosphoribosyltransferase; D014541:Uridine Kinase; D009703:Nucleoside-Phosphate Kinase; D003564:Cytidine Deaminase; D008025:Ligases; D014544:Uridine Triphosphate