Detection of virus and cellular-determined antigens in situ using [125I]protein A and autoradiography.
The present paper describes the use of [125I]Protein A, isolated from Staphylococcus aureus, in detecting antigen-antibody complexes by autoradiography on single cells. the method is relatively quick, reproducible, potentially more sensitive than immunofluorescence, and should be useful in combination with conventional radioimmuno-assays. We have used it to detect the cellular expression of IgM, kappa, lambda, and beta 2-microglobulin, as well as the expression of Epstein-Barr Virus (EBV)-associated antigens expressed in human lymphoblastoid cell lines.
D000956:Antigens, Viral; D001345:Autoradiography; D002213:Capsid / Q000276:immunology; D002460:Cell Line; D002478:Cells, Cultured; D004854:Herpesvirus 4, Human / Q000276:immunology*; D006801:Humans; D007075:Immunoglobulin M; D007457:Iodine Radioisotopes; D013205:Staphylococcal Protein A / Q000494:pharmacology*; D001613:beta 2-Microglobulin
D000956:Antigens, Viral; D007075:Immunoglobulin M; D007457:Iodine Radioisotopes; D013205:Staphylococcal Protein A; D001613:beta 2-Microglobulin