A simple and high yield purification of Crotalus adamanteus phospholipases A2.
A new, high yield, procedure for the purification of the phospholipases A2 of Crotalus adamanteus venom is described. The procedure involves precipitation of the bulk of venom proteins with 50% isopropanol, precipitation of the enzymes from the isopranol soluble material with neodynium chloride, and final purification on DEAE cellulose. An overall yield of approximately 80% is achieved.
D000433:1-Propanol; D002118:Calcium; D011232:Chemical Precipitation; D002848:Chromatography, DEAE-Cellulose; D006863:Hydrogen-Ion Concentration; D009354:Neodymium; D010740:Phospholipases / Q000302:isolation & purification*; D012910:Snake Venoms; D014688:Venoms
D012910:Snake Venoms; D014688:Venoms; D009354:Neodymium; D000433:1-Propanol; D010740:Phospholipases; D002118:Calcium