Fluorescence polarization of DPH-labeled cells adsorbing viruses and its diagnostic potential.
Mammalian or avian cells were labeled with a fluorescent probe DPH (1,6-diphenyl-1,3,5-hexatriene). Within a few minutes after adsorption of various naked and enveloped viruses, the degree of fluorescence polarization (P) of the DPH embedded in the adsorbing cells as measured at 37 degrees C, was reduced, a finding indicating a decrease in the microviscosity of the lipids in the cell membrane. This change of fluidity was proportional to the concentration of the adsorbing virus and could be abolished or inhibited by homologous specific antiviral sera, but not by heterologous sera. Potential use of fluorescence polarization tests is described for titration of virus concentration, as well as for serological identification of a virus.
D000327:Adsorption; D000914:Antibodies, Viral / Q000032:analysis; D002460:Cell Line; D005454:Fluorescence Polarization / Q000379:methods; D007106:Immune Sera; D008560:Membrane Fluidity; D008563:Membrane Lipids / Q000502:physiology; D017955:Poliovirus / Q000254:growth & development*; D018108:Respirovirus / Q000254:growth & development*; D014721:Vesicular stomatitis Indiana virus / Q000254:growth & development*; D014780:Viruses / Q000302:isolation & purification; D014783:Viscosity
D000914:Antibodies, Viral; D007106:Immune Sera; D008563:Membrane Lipids