Transformation and T antigen induction by linearized BK virus DNA.
Linearized unit length DNA obtained after cleavage of the supercoiled DNA of the human papovavirus BKV by PstI (.031) induced transformation and T antigen less efficiently than DNA cleaved by EcoRI (0.0), BamHI (0.98), KpnI (0.90) or HhaI (0.73). BKV DNA cleaved by XbaI (0.27 and 0.95) did not induce T antigen.
D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis*; D001739:BK Virus / Q000235:genetics*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004278:DNA, Superhelical / Q000378:metabolism; D004279:DNA, Viral / Q000235:genetics* / Q000378:metabolism; D005814:Genes, Viral; D000882:Haplorhini; D006801:Humans; D007668:Kidney; D011120:Polyomavirus / Q000235:genetics*; D051381:Rats
D000951:Antigens, Neoplasm; D004278:DNA, Superhelical; D004279:DNA, Viral; D004262:DNA Restriction Enzymes