Human brain in tissue culture. II. Studies of long-term cultures.
This paper describes the techniques used to maintain and reconstitute from storage adult human brain cells in culture. Growth characteristics, cell morphology, lifespan, and karyotypic analysis of cell lines derived from patients with multiple sclerosis (MS), non-MS neurologic diseases, and normal brains are compared.
D000375:Aging; D000690:Amyotrophic Lateral Sclerosis / Q000473:pathology; D001921:Brain / Q000166:cytology*; D002460:Cell Line; D002470:Cell Survival; D002478:Cells, Cultured; D002831:Choroid Plexus / Q000166:cytology; D005615:Freezing; D006128:Growth; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007621:Karyotyping; D008722:Methods; D009103:Multiple Sclerosis / Q000473:pathology; D009900:Optic Nerve / Q000166:cytology; D013696:Temperature; D013997:Time Factors; D014402:Tuberous Sclerosis / Q000473:pathology