The partial purification and properties of a human erythrocyte 4-nitroacetophenone reductase.
1. A soluble enzyme which catalyses the NADPH-dependent reduction of 4-nitroacetophenone to 4-nitrophenylmethylcarbinol has been partially purified from human erythrocytes. 2.inter-individual or intra-individual differences in the enzymic activity were small except for very low activity observed in one subject with glucose 6-phosphate dehydrogenase deficiency resulting in decreased levels of NADPH. 3. The enzyme was inactivated above 50 degrees or on storage at 4 degrees for longer than 24 h. The pH optimum was between 7-0-8-0. 4. the enzyme has been differentiated from NADPH-methaemoglobin reductase, NADPH-cytochrome c reductase, glutathione reductase, alpha,beta-unsaturated ketone reductase and aromatic alpha-keto acid reductase activities, but similarities exist between this enzyme and a rabbit kidney cortex aromatic aldehyde/ketone reductase.
D000098:Acetophenones / Q000378:metabolism; D000645:Ammonium Sulfate; D000818:Animals; D001597:Benzylidene Compounds; D002250:Carbon Radioisotopes; D005591:Chemical Fractionation; D002848:Chromatography, DEAE-Cellulose; D042966:Cytochrome-B(5) Reductase / Q000378:metabolism; D004912:Erythrocytes / Q000201:enzymology*; D005980:Glutathione Reductase / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007658:Ketone Oxidoreductases / Q000097:blood* / Q000378:metabolism; D008297:Male; D009249:NADP / Q000378:metabolism; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D009574:Nitro Compounds; D010084:Oxidation-Reduction; D013696:Temperature
D000098:Acetophenones; D001597:Benzylidene Compounds; D002250:Carbon Radioisotopes; D009574:Nitro Compounds; D009249:NADP; D007658:Ketone Oxidoreductases; D042966:Cytochrome-B(5) Reductase; D009251:NADPH-Ferrihemoprotein Reductase; D005980:Glutathione Reductase; D000645:Ammonium Sulfate