New saccharogenic determination of alpha-amylase in serum and urine.
I describe a new kinetic enzymatic saccharogenic method for assaying alpha-amylase in human serum and urine. alpha-Amylase liberates maltose from starch. This is successively acted on by alpha-glucosidase, mutarotase, and glucose dehydrogenase. The resulting conversion of NAD+ to NADH, measured at 340 nm, during a 20-min incubation reflects amylase activity. Endogenous glucose is destroyed before measurement of amylase activity is begun.
D000681:Amylases / Q000032:analysis*; D002238:Carbohydrate Epimerases; D005948:Glucose Dehydrogenases; D006801:Humans; D007700:Kinetics; D008722:Methods; D009243:NAD / Q000032:analysis; D000516:alpha-Amylases / Q000032:analysis* / Q000097:blood / Q000652:urine; D000520:alpha-Glucosidases
D009243:NAD; D005948:Glucose Dehydrogenases; D000681:Amylases; D000516:alpha-Amylases; D000520:alpha-Glucosidases; D002238:Carbohydrate Epimerases; C019646:aldose 1-epimerase