title: The secretion of enzymes into the pericellular environment.
abstract: Connective tissue cells are capable of both synthesizing and degrading the macromolecular components of the extracellular matrix. The degradation of proteoglycan and collagen has been shown to be associated with the extracellular release of proteolytic enzymes, some of which are of lysosomal origin. The identity in carilage of two previously unrecognized proteases, capable of proteoglycan breakdown (CPGases), has recently been achieved by the use of a new assay for proteoglycan degradation. These enzymes have been shown to be synthesized and released in response to vitamin A. The third proteoglycan degrading enzyme of connective tissue cells, cathepsin D, has been located in the pericellular environment by trapping with specific antibody and the pattern of release studied in organ culture, experimental arthritis and in human rheumatoid tissues. The secretion of this enzyme and possibly also of the other CPGases is thought to be of importance in the local (pericellular) turnover of matrix macromolecules and, in association with collagenase, to be the cause of the excessive degradation in the pannus erosion of articular cartilage in rheumatoid arthritis.
mesh_terms: D000818:Animals; D001172:Arthritis, Rheumatoid / Q000201:enzymology; D002356:Cartilage / Q000378:metabolism*; D002403:Cathepsins / Q000378:metabolism*; D004790:Enzyme Induction; D005110:Extracellular Space / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D008247:Lysosomes / Q000201:enzymology; D010447:Peptide Hydrolases / Q000096:biosynthesis / Q000378:metabolism*; D011509:Proteoglycans / Q000378:metabolism; D011817:Rabbits; D013582:Synovial Fluid / Q000166:cytology / Q000201:enzymology; D014801:Vitamin A / Q000494:pharmacology
chemical_list: D011509:Proteoglycans; D014801:Vitamin A; D002403:Cathepsins; D010447:Peptide Hydrolases