title: [Isolation and some properties of exoribonuclease from the cell nuclei of the rat liver].
abstract: Exoribonuclease from rat liver cell nuclei was isolated and purified using selective extraction at pH 8.0, salting out with ammonium sulfate (30-50% saturation) and chromatography on DEAE-Sephadex A-50. The enzyme has the pH optimum 7.4-7.6, it requires Mg-2+. It catalyses the gradual removal of ribonucleoside-5'-monophosphates from synthetic polynucleotides and RNA; it preferably attacks single-stranded polynucleotides and is less efficient when hydrolysing poly-stranded helical polymers. It is supposed that exoribonuclease can participate in the destruction of non-informative regions in new-formed hyper-molecular RNA of cell nuclei.
mesh_terms: D000818:Animals; D002458:Cell Fractionation; D002467:Cell Nucleus / Q000201:enzymology*; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D002855:Chromatography, Thin Layer; D004355:Drug Stability; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation; D005092:Exonucleases / Q000302:isolation & purification* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007202:Indicators and Reagents; D007700:Kinetics; D008099:Liver / Q000166:cytology*; D008854:Microscopy, Electron; D011063:Poly A-U / Q000378:metabolism; D011072:Poly U / Q000378:metabolism; D051381:Rats; D012260:Ribonucleases / Q000302:isolation & purification* / Q000378:metabolism; D013329:Structure-Activity Relationship
chemical_list: D007202:Indicators and Reagents; D011063:Poly A-U; D011072:Poly U; D005092:Exonucleases; D012260:Ribonucleases
vernacular_title: Vydelenie i nekotorye svoĭstva ékzoribonukleazy iz iader kletok pecheni krys