A murine sarcoma virus-associated protein kinase: interaction with actin and microtubular protein.
A low molecular weight (LMW) protein phosphokinase enzyme that binds to actin has been isolated from murine sarcoma virions; this kinase activity is not present in nontransforming murine leukemia viruses. Sephadex G-75 gel filtration and affinity chromatography on actin-Sepharose conjugates allow a significant level of purification of this enzyme. The enzyme associates with microtubular proteins and inhibits the in vitro polymerization of microtubules. This study represents the first isolation of a sarcoma virus-associated protein that possesses the ability to interact directly with two major components of the cytoskeletal system.
D000199:Actins / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral; D017988:Gammaretrovirus / Q000201:enzymology*; D006023:Glycoproteins / Q000378:metabolism*; D051379:Mice; D008870:Microtubules / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism; D010766:Phosphorylation; D010774:Phosvitin / Q000378:metabolism; D011485:Protein Binding; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases / Q000378:metabolism*; D009053:Sarcoma Viruses, Murine / Q000201:enzymology*; D014404:Tubulin / Q000378:metabolism*
D000199:Actins; D006023:Glycoproteins; D010750:Phosphoproteins; D047428:Protein Kinase Inhibitors; D014404:Tubulin; D010774:Phosvitin; D011494:Protein Kinases