Fatty acid stimulation of membrane phosphatidylinositol hydrolysis by brain phosphatidylinositol phosphodiesterase.
The hydrolysis of membrane-bound phosphatidylinositol in rat liver microsomal fraction by the soluble phosphatidylinositol phosphodiesterase from rat brain was markedly stimulated by oleic acid or arachidonic acid. The stimulation did not require added calcium, although it was abolished by EDTA. Lysophosphatidylcholine also totally suppressed the stimulation. A possible role for the fatty acid content of a membrane in controlling phosphatidylinositol turnover is suggested.
D000818:Animals; D001921:Brain / Q000201:enzymology; D002462:Cell Membrane / Q000378:metabolism*; D005227:Fatty Acids / Q000494:pharmacology*; D006868:Hydrolysis; D066298:In Vitro Techniques; D007425:Intracellular Membranes / Q000378:metabolism; D008862:Microsomes, Liver / Q000378:metabolism; D010716:Phosphatidylinositols / Q000378:metabolism*; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D051381:Rats; D013268:Stimulation, Chemical
D005227:Fatty Acids; D010716:Phosphatidylinositols; D010727:Phosphoric Diester Hydrolases