The induction of virus-specific cytotoxic T lymphocytes with solubilized viral and membrane proteins.
Reconstituted membranes were prepared from detergent solubilized P815 (H-2d) tumor cell membranes and solubilized Sendai virus protein. These reconstituted membranes stimulated a virus-specific H-2-restricted secondary CTL response. Stimulating activity was dependent upon the presence of both viral and P815 protein in the same membrane and was restricted to the H-2 specificity present in the reconstituted membrane. Liposomes prepared from solubilized Sendai virus proteins and partially purified H-2 alloantigen also had activity for CTL induction. The results demonstrate the feasibility of using detergent solubilized membrane proteins to study antigen recognition by virus-specific, H-2 restricted cytolytic T lymphocytes.
D000818:Animals; D003602:Cytotoxicity, Immunologic; D006183:H-2 Antigens; D007156:Immunologic Memory; D008081:Liposomes; D008565:Membrane Proteins / Q000276:immunology*; D051379:Mice; D008954:Models, Biological; D010222:Parainfluenza Virus 1, Human / Q000276:immunology*; D012995:Solubility; D013601:T-Lymphocytes / Q000276:immunology*; D014764:Viral Proteins / Q000276:immunology*
D006183:H-2 Antigens; D008081:Liposomes; D008565:Membrane Proteins; D014764:Viral Proteins