Studies on specific antigen content in different fractions of preparations from BLV-producing cell cultures.
Different protein fractions of BLV-antigens were examined with serological and chemical methods. The fractions of ether resistant and dual antigens were obtained by means of QAE-50A Sephadex chromatography and the fractions of gp-antigen were collected in the course of the affinity chromatography on Concanavalin A-Sepharose. All protein fractions examined by means of polyacrylamide gels electrophoresis occurred to be heterogenous. Some of them showed positive RID or GID reactions with sera of cattle or sheep affected with leukosis. By means of thinlayer chromatography on Sephadex, there were estimated following molecular weights of proteins in the serologically positive fractions: 12.500, 67.000, 70.000 and 150.000.
D000956:Antigens, Viral / Q000032:analysis* / Q000302:isolation & purification; D002478:Cells, Cultured; D006023:Glycoproteins / Q000276:immunology / Q000302:isolation & purification; D005779:Immunodiffusion; D001909:Leukemia Virus, Bovine / Q000254:growth & development / Q000276:immunology*; D012190:Retroviridae / Q000276:immunology*
D000956:Antigens, Viral; D006023:Glycoproteins