[Purification from a total cellular lysate of a protein modifying the sensitivity of Na+/K+ ATPase to ouabain].
A protein (32,000 dalton) has been purified from M2FS cells derived from the murine plasmocytoma MOPC 173. Like tropomyosin, this protein when added with Ca++ to EDTA-treated plasma membranes prepared from the same cell, induced a drastic increase in the Na+/K+ atpase resistance to ouabain.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology; D004492:Edetic Acid / Q000494:pharmacology; D046911:Macromolecular Substances; D051379:Mice; D008970:Molecular Weight; D010042:Ouabain / Q000494:pharmacology*; D010954:Plasmacytoma; D011506:Proteins / Q000302:isolation & purification / Q000502:physiology*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D014335:Tropomyosin
D046911:Macromolecular Substances; D011506:Proteins; D014335:Tropomyosin; D010042:Ouabain; D004492:Edetic Acid; D000254:Sodium-Potassium-Exchanging ATPase; D002118:Calcium
Purification d'une protéine modifiant la sensibilité de la Na+/K+ ATPase à l'ouabaïne à partir d'un lysat cellulaire total.