Splicing and the formation of stable RNA.
To determine whether RNA splicing plays an obligatory role in gene expression, we have constructed a series of SV40-transducing viruses carrying various combinations of splice junctions derived from the viral genome and a mouse globin gene. All of the viruses that retain at least one functional splice junction, derived from either the viral or the mouse genome, encode stable hybrid RNAs. In contrast, a virus from which all the splice junctions have been removed fails to produce any detectable stable RNA. These results suggest that splicing is a prerequisite for stable RNA formation.
D000818:Animals; D005822:Genetic Vectors; D005914:Globins / Q000235:genetics; D051379:Mice; D009694:Nucleic Acid Precursors / Q000378:metabolism*; D009876:Operon; D012313:RNA / Q000378:metabolism*; D011995:Recombination, Genetic; D013539:Simian virus 40 / Q000235:genetics*; D014158:Transcription, Genetic
D009694:Nucleic Acid Precursors; D012313:RNA; D005914:Globins