Spontaneous and induced cytotoxic properties of human adherent mononuclear cells: killing of non-sensitized and antibody-coated non-erythroid cells.
A method is described which demonstrates that human adherent mononuclear cells have a strong capacity to kill human nucleated target cells. Cytotoxicity was measured by the release of 51chromium from two established cell lines. Freshly prepared monolayers killed both non-sensitized and anti-body coated K562 and CLA-4 target cells. These spontaneous events decreased after short term culture. A second peak of cytotoxic activity was induced by activating the effector cells with endotoxin-treated serum or phytohaemagglutinin. Cytotoxicity was inhibited by silica particles. Studies with alpha-naphthyl esterase showed that 95% of freshly prepared cells and 99% of monolayers cultured for 68 h were monocytes. These studies suggest that adherent monocytes are the predominant cytotoxic effector cell although a contributing effect by the small numbers of contaminating lymphocytes can not be excluded. Evaluation of monocyte cytotoxicity should have a useful role in clinical investigation.
D000920:Antibody-Dependent Cell Cytotoxicity / Q000187:drug effects; D002448:Cell Adhesion; D002460:Cell Line; D003602:Cytotoxicity, Immunologic / Q000187:drug effects; D004731:Endotoxins / Q000494:pharmacology; D006801:Humans; D009000:Monocytes / Q000276:immunology*; D009374:Neoplasms, Experimental / Q000276:immunology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D012822:Silicon Dioxide / Q000494:pharmacology
D004731:Endotoxins; D010835:Phytohemagglutinins; D012822:Silicon Dioxide