Latent cytomegalovirus infection of BALB/c mouse spleens detected by an explant culture technique.
Latent murine cytomegalovirus (MCMV) infection of BALB/c mouse spleens was studied using several methods including an explant tissue culture technique, co-cultivation on allogeneic and syngeneic cell cultures and nucleic acid hybridization. BALB/c mice experience latent infection which persists for at least 6 months and involves only a small fraction of spleen cells. The explant culture technique proved to be much more sensitive than other methods for detecting latent infection of lymphoid tissues.
D000818:Animals; D002478:Cells, Cultured; D046508:Culture Techniques; D003587:Cytomegalovirus / Q000254:growth & development*; D003586:Cytomegalovirus Infections / Q000382:microbiology*; D004279:DNA, Viral / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D009693:Nucleic Acid Hybridization; D013154:Spleen / Q000382:microbiology*; D013997:Time Factors
D004279:DNA, Viral