Ternary solvents to investigate proteins at zub-zero temperatures.
Mixtures of water, ethylene glycol and methanol in different volume ratios have been selected to carry out kinetics of enzyme reactions at sub-zero temperatures with the intention to reduce maximally the viscosity. Density, viscosity and dielectric constant values of these mixtures as a function of temperature are reported, as well as values of the protonic activity of several buffers under such conditions. A procedure to avoid or delay the eventual damaging effect of methanol on proteins is described.
D000085:Acetates; D002021:Buffers; D002101:Cacodylic Acid; D003080:Cold Temperature; D003720:Densitometry; D004798:Enzymes; D005026:Ethylene Glycols; D005615:Freezing; D005998:Glycine; D006863:Hydrogen-Ion Concentration; D000432:Methanol; D010710:Phosphates; D011506:Proteins; D011522:Protons; D013696:Temperature; D014325:Tromethamine; D014783:Viscosity; D014867:Water
D000085:Acetates; D002021:Buffers; D004798:Enzymes; D005026:Ethylene Glycols; D010710:Phosphates; D011506:Proteins; D011522:Protons; D014325:Tromethamine; D014867:Water; D002101:Cacodylic Acid; D005998:Glycine; D000432:Methanol