Protein kinases in brown adipose tissue of developing rats.  I. GTP as nucleotide substrate for histone phosphorylation.
100 000 times g soluble extracts from interscapular brown adipose tissue catalyzed the transfer of the terminal phosphoryl group from GTP to histone.  Maximal velocity was achieved only with both cyclic AMP and ATP present.  The cyclic AMP dose-response curve was the same as for the ATP-utilizing enzyme, with maximum stimulation at 0.5 muM.  ATP (1--100muM) increased the rate of histone phosphorylation with GTP as the radioactive substrate.  Higher concentrations had a dilution effect similar to that of GTP on the ATP-utilizing enzyme.  Similar effects were observed with ADP and AMP.  The apparent Km values for histone were the same with both GTP and ATP as nucleotide substrates.  The effects of pH, purified beef muscle kinase inhibitor and of NaCl were also the same.  Maximum velocities of histone phosphorylation from ATP and those from GTP were almost the same in brown fat of all age groups testes,  Separated on histone-Sepharose, the GTP-utilizing activity was absolutely dependent on the re-addition of the ATP-utilizing enzyme (a linear relationship with a slope of approx. 0.95).  An extremely active nucleotide phosphotransferase activity was found in the same subcellular fraction.  The rate of equilibration of the gamma-32-P between GTP and ATP could account for all the histone phosphorylation with [gamma-32-P] GTP.  It is concluded that, in spite of the presence of nucleotide phosphotransferase and ATP-protein kinase activities, a direct transfer from GTP to a protein substrate cannot be excluded.  Also, histone may not be the natural protein acceptor for GTP-linked phosphorylation.
D000227:Adenine Nucleotides / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000378:metabolism; D002001:Adipose Tissue, Brown / Q000201:enzymology*; D000375:Aging; D000818:Animals; D002417:Cattle; D002642:Chick Embryo; D000242:Cyclic AMP / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006160:Guanosine Triphosphate / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D006656:Protamine Kinase / Q000378:metabolism*; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases / Q000378:metabolism*; D051381:Rats; D012965:Sodium Chloride / Q000494:pharmacology
D000227:Adenine Nucleotides; D047428:Protein Kinase Inhibitors; D012965:Sodium Chloride; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D000242:Cyclic AMP; D011494:Protein Kinases; D006656:Protamine Kinase