Fluorometric assay of bethanidine in plasma.
A fluorometric method for determining bethanidine in blood plasma is described. The bethanidine is extracted into chloroform, a drug-dye complex with eosin Y is formed, and the fluorescence is measured (excitation, 535 nm; fluorescence, 560 nm). The assay detects 0.02 muM bethanidine (4 ng/ml) in plasma. The relative fluorescence of several body constituents and antihypertensive drugs is negligible. The plasma levels of bethanidine in four hypertensive patients receiving this drug were measured.
D000818:Animals; D001627:Bethanidine / Q000097:blood*; D005260:Female; D005470:Fluorometry; D006146:Guanidines / Q000097:blood*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008253:Macaca mulatta; D008297:Male; D008875:Middle Aged; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors
D006146:Guanidines; D001627:Bethanidine