The isolation and characterization of lactoferrin from sow milk and boar seminal plasma.
Lactoferrin isolated from sow milk (about 0-6 mg/ml) was shown to be chromatographically homogeneous, an observation supported by electrophoresis and by reaction against monospecific anti-lactoferrin antiserum. Isoelectric focusing showed multiple forms of the protein (i.e.p., 9-3 to 10-0) converted by neuraminidase to one form (i.e.p., 9-65). Boar seminal plasma contains immunologically identical lactoferrin (0-1 to 0-5 mg/ml) which binds strongly to boar spermatozoa.
D000645:Ammonium Sulfate; D000818:Animals; D001826:Body Fluids / Q000032:analysis; D002021:Buffers; D002850:Chromatography, Gel; D004586:Electrophoresis; D004822:Epididymis / Q000032:analysis; D005260:Female; D006863:Hydrogen-Ion Concentration; D007106:Immune Sera; D007122:Immunoelectrophoresis; D007158:Immunologic Techniques; D007501:Iron; D007525:Isoelectric Focusing; D007781:Lactoferrin / Q000302:isolation & purification*; D007782:Lactoglobulins / Q000302:isolation & purification*; D008297:Male; D008892:Milk / Q000032:analysis*; D011817:Rabbits / Q000276:immunology; D012661:Semen / Q000032:analysis*; D012669:Seminal Vesicles / Q000378:metabolism; D013552:Swine / Q000378:metabolism*; D014462:Ultrafiltration
D002021:Buffers; D007106:Immune Sera; D007782:Lactoglobulins; D007501:Iron; D007781:Lactoferrin; D000645:Ammonium Sulfate