Cyclic adenosine 3',5'-monophosphate regulation of the bacteriophage T6/colicin K receptor in Escherichia coli.
Mutant strains of Escherichia coli unable to synthesize cyclic adenosine 3',5'-monophosphate (cAMP) or the cyclic adenosine monophosphate receptor protein (CRP) were more resistant than wild-type cells to infection by bacteriophage T6. This resistance was found to be associated with the decreased production of specific T6 receptor protein (also the colicin K receptor) located in the outer membrane protein fraction of these cells. Transcription of this particular outer membrane protein was regulated by the cAMP-CRP complex. A novel affinity technique coupled with sodium dodecyl sulfate-polyacrylamide gel electrophoresis was used in these investigations.
D001426:Bacterial Proteins / Q000096:biosynthesis*; D003087:Colicins; D000242:Cyclic AMP / Q000502:physiology*; D004926:Escherichia coli / Q000378:metabolism*; D009154:Mutation; D011953:Receptors, Cyclic AMP / Q000502:physiology; D011955:Receptors, Drug / Q000096:biosynthesis*; D011991:Receptors, Virus / Q000096:biosynthesis*; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D003087:Colicins; D011953:Receptors, Cyclic AMP; D011955:Receptors, Drug; D011991:Receptors, Virus; D000242:Cyclic AMP