Increase of interferon antiviral activity by exogenous cyclic adenosine-3':5'-monophosphate (cAMP).
Some effects of cAMP on replication of Semliki Forest Virus in chick embryo fibroblast cell cultures are described. Depending on concentration, the incorporation of [3H]-uridine into viral RNA or the formation of plaque-forming units is inhibited; the highest concentration tested was 8 mM. Cyclic AMP has an effect of its own and increases the Interferon action in the lower concentration ranges of Interferon (up to 1 unit/ml). The effect of cyclic AMP is fast, needs no induction and is also visible in late phases of viral replication. However, these experiments do not establish a causal relation between cAMP and Interferon.
D000242:Cyclic AMP / Q000494:pharmacology*; D004351:Drug Resistance; D004357:Drug Synergism; D007372:Interferons / Q000494:pharmacology*; D007700:Kinetics; D012367:RNA, Viral / Q000096:biosynthesis; D012672:Semliki forest virus / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors; D014529:Uridine / Q000378:metabolism; D014779:Virus Replication / Q000187:drug effects
D012367:RNA, Viral; D007372:Interferons; D000242:Cyclic AMP; D014529:Uridine