Relationship between the intracellular cyclic adenosine 3':5'-monophosphate level of tumor cells and their sensitivity to killing by antibody and complement.
Metabolic inhibitors which have been shown to increase the sensitivity of the guinea pig hepatoma, line 10, to antibody-guinea pig complement killing were tested for their effect on the intracellular cyclic adenosine 3':5'-monophosphate (cAMP) level of the line 10 cells. It was found that cells rendered sensitive to antibody-guinea pig complement killing following drug treatment showed a decrease in their intracellular cAMP levels. Increased susceptibility to lysis was always associated with decreased intracellular cAMP levels. Optimal doses of certain inhibitors effectively increased the sensitivity of the cells to complement-dependent lysis and decreased the intracellular cAMP levels. Other drugs which did not increase sensitivity of the cells to lysis also failed to decrease the intracellular cAMP levels. No experimental conditions were found in which intracellular cAMP levels were decreased without an increase in the susceptibility of the cells to antibody-guinea pig complement killing.
D000818:Animals; D000906:Antibodies / Q000008:administration & dosage*; D002470:Cell Survival / Q000187:drug effects; D003165:Complement System Proteins; D000242:Cyclic AMP / Q000378:metabolism*; D003609:Dactinomycin / Q000494:pharmacology; D004317:Doxorubicin / Q000494:pharmacology; D006168:Guinea Pigs; D007109:Immunity / Q000187:drug effects*; D008114:Liver Neoplasms, Experimental / Q000276:immunology / Q000378:metabolism* / Q000628:therapy; D008297:Male; D008937:Mitomycins / Q000494:pharmacology; D011691:Puromycin / Q000494:pharmacology
D000906:Antibodies; D008937:Mitomycins; D003609:Dactinomycin; D011691:Puromycin; D004317:Doxorubicin; D003165:Complement System Proteins; D000242:Cyclic AMP