[Interference of antioxidants E/O of some free radical "scavengers" with the activity of glucose-6-phosphatase after administration of carbon tetrachloride].
G-6-Pase activity was investigated in the microsomal fraction from rat liver in the presence of carbon tetrachloride and/or propyl gallate (PG), reduced glutathione (GSH) and superoxide dismutase. Results obtained "in vitro" demonstrated that CCl4 induced a 60% inhibition of the microsomal enzyme activity. Moreover, a marked inhibition of G-6-Pase activity was found also when propyl gallate and reduced glutathione were added, at different concentrations, to incubation mixture. In addition, these drugs were unable to interfere with the dangerous effect exerted on the enzymatic activity by the haloalkane. Additional experiments carried out "in vivo" with propyl gallate produced evidence that intraperitoneal administration of the antioxidant was followed by a significant inhibition of G-6-Pase activity, while the damaging action of CCl4 was unaffected. Some possible explanations of these results are reported.
D000818:Animals; D002252:Carbon Tetrachloride Poisoning / Q000201:enzymology*; D005609:Free Radicals; D005707:Gallic Acid / Q000031:analogs & derivatives*; D005952:Glucose-6-Phosphatase / Q000037:antagonists & inhibitors*; D005978:Glutathione / Q000494:pharmacology*; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology*; D011435:Propyl Gallate / Q000494:pharmacology*; D051381:Rats; D013482:Superoxide Dismutase / Q000494:pharmacology
D005609:Free Radicals; D005707:Gallic Acid; D011435:Propyl Gallate; D013482:Superoxide Dismutase; D005952:Glucose-6-Phosphatase; D005978:Glutathione
Inferenza di antiossidanti E/O di taluni "scavengers" di radicali liberi con l'attivita' della glucoso-6-fosfatasi dopo somministrazione di tetracloruro di carbonio.