[Purification of coronaviruses from contaminated cattle feces].
A scheme was worked out for the obtaining of purified Corona viruses from fecal extracts, which include: centrifugation in an discontinued gradient of sucrose 20--45 per cent, gel filtration through a Sephadex 4B column, and centrifugation in a linear gradient of sucrose 10--50 per cent. By means of such purification the virions remain morphologically intact in sucrose density of 1.11--1.14 gr/cm3, containing no ballast proteins. As many as five polypeptides are demonstrated in polyacrylamide electrophoresis.
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D002499:Centrifugation, Density Gradient; D002501:Centrifugation, Zonal; D002850:Chromatography, Gel; D003332:Coronaviridae / Q000302:isolation & purification*; D003333:Coronaviridae Infections / Q000382:microbiology / Q000662:veterinary*; D004591:Electrophoresis, Polyacrylamide Gel; D004751:Enteritis / Q000662:veterinary*; D005243:Feces / Q000382:microbiology*
Predchistvane na koronavirusi ot kontaminirani govezhdi fekali.