[The polyphosphate synthetase of Saccharomyces cerevisiae].
The polyphosphate-synthetase, isolated from a homogenate of phosphate starved cells, catalyses the synthesis of linear polyphosphates from orthophosphate. It is localized in the membrane fraction which deposits between 400 and 1000 X g; its optimal pH is 7.1; its KM toward orthophosphate is 4.0 X 10(-4) M; ATP stimulates the reaction. The enzyme synthezises especially polyphosphates with short chain length.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D002458:Cell Fractionation; D002848:Chromatography, DEAE-Cellulose; D004789:Enzyme Activation; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D008566:Membranes / Q000201:enzymology; D008970:Molecular Weight; D010710:Phosphates / Q000378:metabolism; D010770:Phosphotransferases / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D012995:Solubility
D010710:Phosphates; D000255:Adenosine Triphosphate; D010770:Phosphotransferases; D008274:Magnesium
Recherches sur la polyphosphate-synthétase de Saccharomyces cerevisiae