A 3'-deoxymononucleotide-producing nuclease from the marine sponge Verongia aerophoba. II. General properties.
The properties of a 3'-deoxymono-nucleotide-producing nuclease highly purified from the marine sponge Verongia aerophoba are described. The hydrolysis of heat-dentured DNA-proceeds at about five fold the rate of native, double-stranded DNA. The enzyme behaves as an exonuclease by producing 3'-deoxymononucleotides. Kinetic analysis by means of initial hyperchromicity at 260 nm indicates that the enzyme requries 120 mmol/l of mono-valent and 30 mmol/l of divalent cations with a clear dependence on ionic radius. The enzyme has  a pH optimum at 4.7, the pH value of the isoelectric point is 6.1. The molecular weight has been evaluated at 62,000.
D000818:Animals; D002413:Cations, Divalent; D002414:Cations, Monovalent; D003851:Deoxyribonucleases / Q000378:metabolism*; D005092:Exonucleases / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D007700:Kinetics; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D012964:Sodium / Q000494:pharmacology; D013696:Temperature; D013997:Time Factors
D002413:Cations, Divalent; D002414:Cations, Monovalent; D012964:Sodium; D003851:Deoxyribonucleases; D005092:Exonucleases