Studies of the leukemic cell associated antigen(s) in mice with myeloid leukemia (Graffi), using the immunoperoxidase techniques.
Thymus, spleen and lymph nodes from myeloid leukemic mice (Graffi) were examined by light and electron microscope using immunoperoxidase techniques. As immune sera autologous sera were applied. The percentage of the labeled leukemic cells ranged from 30 to 60. It was established that leukemic cell associated antigen(s) was located mainly on the plasma and nuclear membranes. Occasionally this antigen(s) was observed in some areas of the endoplasmic reticulum, on separate cytoplasmic vesicles, on the budding viruses and on the chromatin. In addition, weakly expressed antigen(s) was located in cytosol. The mature virus particles were not labeled with peroxidase.
D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis*; D002462:Cell Membrane / Q000276:immunology; D007124:Immunoenzyme Techniques; D009052:Leukemia Virus, Murine / Q000276:immunology*; D007942:Leukemia, Experimental / Q000276:immunology* / Q000648:ultrastructure; D008198:Lymph Nodes / Q000276:immunology; D051379:Mice; D009685:Nuclear Envelope / Q000276:immunology; D013154:Spleen / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology
D000951:Antigens, Neoplasm