Selective and unidirectional membrane redistribution of an H-2 antigen with an antibody-clustered viral antigen: relationship to mechanisms of cytotoxic T-cell interactions.
We have studied the co-redistribution of vesicular stomatitis virus (VSV) antigen and of individual H-2 antigens on the surfaces of mouse cells, and in parallel we have also used these VSV-infected cells as targets in cytotoxic T-cell killing experiments. Antibody-induced patching and capping of the VSV antigen caused an extensive co-patching and co-capping of the H-2Kb antigen but not of the H-2Db antigen. In reciprocal experiments, the antibody-induced patching of the H-2Kb or H-2Db antigen did not result in a co-patching of the VSV antigen. Radioimmunoassays showed that the relative numbers of H-2Kb, H-2Db, and VSV antigens on the surfaces of the cells exhibiting such nonreciprocal co-redistributions were closely similar. Furthermore, the H-2 restricted cytotoxic T-cell lysis of these target cells showed a marked preference for H-2Kb compared to H-2Db compatibility. We propose that the VSV and H-2 antigens are molecularly independent entities in the unpreturbed target cell membrane but that the antibody-induced clustering of the VSV antigen causes a selective and unidirectional co-redistribution (which we designate as syn-capping) of H-2Kb with the VSV antigen clusters. It is suggested that such a T-cell-induced syn-capping process involving an antigen and an H-2 molecule on the target cell may play a critical role in the mechanism of cytotoxic T-cell killing.
D000914:Antibodies, Viral; D000937:Antigen-Antibody Reactions; D000954:Antigens, Surface; D000956:Antigens, Viral; D003602:Cytotoxicity, Immunologic; D006023:Glycoproteins / Q000276:immunology; D006183:H-2 Antigens / Q000276:immunology*; D007152:Immunologic Capping; D008565:Membrane Proteins / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014721:Vesicular stomatitis Indiana virus / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology
D000914:Antibodies, Viral; D000954:Antigens, Surface; D000956:Antigens, Viral; D006023:Glycoproteins; D006183:H-2 Antigens; D008565:Membrane Proteins; D014764:Viral Proteins