Non-linear dynamic phenomena in open reconstituted enzyme systems.
The dynamic pattern of an open reconstituted in-vitro enzyme system containing phosphofructokinase, pyruvate kinase and adenylate kinase has been investigated. The approach is experimentally based on a stirred flow-through reactor. Stationary concentrations of phosphofructokinase and pyruvate kinase are maintained by entrapment in polyacrylamide gel particles. The results fit to a theoretical model based on the initial kinetic responses of the enzymes involved. An S-shaped steady state input characteristic for fructose 6-phosphate has been experimentally obtained.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000263:Adenylate Kinase / Q000378:metabolism*; D004800:Enzymes, Immobilized; D005636:Fructosephosphates / Q000378:metabolism; D008956:Models, Chemical; D010732:Phosphofructokinase-1 / Q000378:metabolism*; D010770:Phosphotransferases / Q000378:metabolism*; D011770:Pyruvate Kinase / Q000378:metabolism*; D011773:Pyruvates / Q000378:metabolism
D004800:Enzymes, Immobilized; D005636:Fructosephosphates; D011773:Pyruvates; D000255:Adenosine Triphosphate; D010770:Phosphotransferases; D010732:Phosphofructokinase-1; D011770:Pyruvate Kinase; D000263:Adenylate Kinase