Gas-liquid chromatographic determination of diethylstilbestrol in molasses-based liquid feed supplements.
A gas-liquid chromatographic (GLC) method is described for the determination of diethylstilbestrol (DES) in molasses-based liquid feed supplements, using dienestrol diacetate as the internal standard. A sample equivalent to 110 mug DES was diluted, acidified with dilute sulfuric acid, extracted with chloroform, and subjected to basic sodium acetate cleanup. The bis-(trimethylsilyl) acetamide derivative was prepared and determined by GLC. Interfering peaks and/or low recoveries were found to be related to emulsions and the procedure incorporates centrifugation to minimize these.
D000821:Animal Feed / Q000032:analysis*; D002725:Chloroform; D002849:Chromatography, Gas; D004054:Diethylstilbestrol / Q000032:analysis*; D005503:Food Additives / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D008722:Methods; D008965:Molasses
D005503:Food Additives; D004054:Diethylstilbestrol; D002725:Chloroform