Relationship between T-antigen and tumor-specific transplantation antigen in simian virus 40-transformed cells.
The simian virus 40 (sv40) tumor antigen (T-antigen) and tumor-specific transplantation antigen (TSTA) have been partially purified and studied to clarify their relationship. The T-antigen and the TSTA were partially purified from nuclei of SV AL/N cells, and SV40-transformed mouse embryo fibroblast line, by precipitation with ammonium sulfate and chromatography on DEAE- and DNA-cellulose. The T-antigen was assayed by complement fixation, and the TSTA was assayed by its ability to immunize mice against SV40-containing ascites tumor cells. When T-antigen- and TSTA-containing preparations were sedimented through sucrose gradients, each antigen had a major peak of activity at a sedimentation coefficient of 6.7 and minor peaks in other regions. Antiserum against T-antigen (from tumor-bearing hamsters) immunoprecipitated the TSTA activity. A preparation of T-antigen from human SV80 cells, which exhibited only one protein band after sodium dodecylsulfate-polyacrylamide gel electrophoresis, had TSTA activity when as little as 0.6 microgram of protein per mouse was used for immunization. These experiments demonstrate that the T-antigen, the product of the SV40 early A gene is capable of inducing specific immunity against transplantation of SV40-transformed tumor cells in mice.
D000951:Antigens, Neoplasm / Q000032:analysis*; D000954:Antigens, Surface; D000956:Antigens, Viral / Q000032:analysis*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D002499:Centrifugation, Density Gradient; D011232:Chemical Precipitation; D006649:Histocompatibility Antigens / Q000032:analysis*; D007106:Immune Sera; D013539:Simian virus 40 / Q000276:immunology*
D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D000956:Antigens, Viral; D006649:Histocompatibility Antigens; D007106:Immune Sera