title: Collagenase in equine cell culture preparation.
abstract: Equine kidney cells disaggregated by treatment with 0.01% collagenase were used in the preparation of primary monolayer cell cultures. The primary cells could be stored for long periods in liquid nitrogen and subsequently subcultivated. These techniques provided a long-term supply of equine kidney cells, free of apparent contamination, from the kidneys of a single fetus.
mesh_terms: D000818:Animals; D002469:Cell Separation; D002478:Cells, Cultured; D005333:Fetus; D006736:Horses; D007668:Kidney; D003012:Microbial Collagenase / Q000494:pharmacology*
chemical_list: D003012:Microbial Collagenase