Genetic and physiological characterization of temperature-sensitive mutants of bluetongue virus.
Complementation studies were carried out, using temperature-sensitive (t-s) mutants of blue-tongue virus (BTV). The results proved to be inconclusive as only low indices of complementation were obtained. No discrepancy was found between the previous classification of these mutants in 6 recombination classes and the complementation data recored. In general, the t-s mutants require a latent growth period of 16-20 h at 28 degrees C and maximum titres can be demonstrated 40-48 h post-infection. One mutant, (F211), however, consistently had a growth lag phase of 32 h. Mutants of the 6 recombination groups were further classified into 2 groups by temperature-shift studies. One calss of mutants expressed their t-s lesion prior to 24 h and the other class only after 24 h post-infection. Mutant F73 was found to be defective in its ability to synthesize ssRNA at a late stage in the replication cycle at the non-permissive temperature.
D001820:Bluetongue virus / Q000145:classification / Q000235:genetics* / Q000254:growth & development; D005814:Genes, Viral; D005816:Genetic Complementation Test; D009154:Mutation; D012367:RNA, Viral / Q000096:biosynthesis; D012087:Reoviridae / Q000235:genetics*; D013696:Temperature
D012367:RNA, Viral