title: The effect of factors causing induction of DNA breaks on transfection of chicken cells by the XC DNA, and the kinetics of appearance of virus-producing cells after transfection.
abstract: The effect of BUdR and 4NQO treatment of the recipient chicken fibroblast cultures on the efficiency of transfection by the XC DNA was investigated. The efficiency of transfection was 2-fold higher when the recipient cultures were incubated in the presence of 10 micrograms BUdR/ml in medium 199 (48%) than when cultures were not treated (25%). The efficiency was not further increased by exposure of BUdR-treated cultures to visible light for 20 min (31%). Growth of BUdR-treated cultures decreased after light irradiation which indicated that BUdR was incorporated into the host cell DNA. Treatment of the recipient cultures with 4NGO at a concentration of 0.4 micrograms/ml for 2 h before transfection exerted only unfavourable effects on transfection efficiency. In cultures positive in transfection, transformation was first detected 3 days after exposure to DNA by the infectious centre assay, with a frequency of 4.76 +/- 6.46 transformed cells per 10(6) cells.
mesh_terms: D015112:4-Nitroquinoline-1-oxide / Q000494:pharmacology; D000818:Animals; D001358:Avian Sarcoma Viruses / Q000235:genetics*; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002472:Cell Transformation, Viral / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D002645:Chickens; D004279:DNA, Viral / Q000235:genetics* / Q000528:radiation effects; D005347:Fibroblasts; D008027:Light; D014162:Transfection / Q000187:drug effects / Q000528:radiation effects
chemical_list: D004279:DNA, Viral; D015112:4-Nitroquinoline-1-oxide; D001973:Bromodeoxyuridine