[A simple micromethod for sphingomyelinase determination (author's transl)].
A rapid and sensitive micromethod for routine determination of sphingomyelinase activity of leukocytes, fibroblasts and hair roots is described. This method makes use of the natural radioactive substrate of the enzyme. The product of the reaction is isolated by direct application of the incubation mixture on silica gel plates followed by ascending chromatography. The sensitivity of the method allows sphingomyelinase determination in less than 0.5 microgram of fibroblast proteins.
D002250:Carbon Radioisotopes; D005347:Fibroblasts / Q000032:analysis; D006197:Hair / Q000032:analysis; D006801:Humans; D007962:Leukocytes / Q000032:analysis; D008722:Methods; D010727:Phosphoric Diester Hydrolases / Q000032:analysis*; D013108:Sphingomyelin Phosphodiesterase / Q000032:analysis*
D002250:Carbon Radioisotopes; D010727:Phosphoric Diester Hydrolases; D013108:Sphingomyelin Phosphodiesterase
Une microméthode simple pour la determination de l'activité sphingomyélinase.