title: ELISA: enzyme-linked immunosorbent assay.
abstract: Similar in design to radioimmunoassay, comparable in sensitivity and specificity but easier, safer, and less expensive, this new diagnostic technique uses enzyme-labeled rather than isotope-labeled reagents. The end point is a color change that can be assessed by colorimetry or with the naked eye. Various techniques of ELISA are described, along with examples of current and potential clinical applications.
mesh_terms: D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D000956:Antigens, Viral / Q000302:isolation & purification; D002417:Cattle; D002648:Child; D002675:Child, Preschool; D003116:Color; D004768:Enterotoxins / Q000302:isolation & purification; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D004926:Escherichia coli / Q000302:isolation & purification; D005759:Gastroenteritis / Q000209:etiology; D006168:Guinea Pigs; D006511:Hepatitis B Antigens / Q000302:isolation & purification; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology; D007223:Infant; D012401:Rotavirus / Q000302:isolation & purification; D014734:Vibrio cholerae / Q000302:isolation & purification
chemical_list: D000906:Antibodies; D000956:Antigens, Viral; D004768:Enterotoxins; D006511:Hepatitis B Antigens; D007074:Immunoglobulin G; D007075:Immunoglobulin M