[ESR study of mitochondria in basal metabolic states and at different levels of ATP-synthetase activity].
Effect of metabolic transitions of mitochondria on the value of ESR-absorption measured at room temperature has been studied. It has been found that the content of free radicals with ESR signal of g = 2.00 and the halfwidth about 15e depends on the metabolic state of mitochondria determined by the load of respiration and phosphorylation systems of mitochondria. The maximal gain of the free radical content is observed in the active state when the maximal load is applied to the phosphorylative chain. An attempt to localize the region of initiation of additional free radicals by means of uncouplers and inhibitors of phosphorylation, as well as by placing mitochondria into the phosphate-free medium has shown that the change in ESR absorption of mitochondrion preparation is in a specific correlation with the change in energization of mitochondria. The analysis of alternative ways for an increase of the free radical fund during energization makes it possible to connect this increase with the work of ATP-syntetase and to suggest that free radicals take part in the ADP phosphorylation by inorganic phosphate, being probably, localized directly on the ADP molecule.
D000244:Adenosine Diphosphate / Q000378:metabolism; D001481:Basal Metabolism; D004140:Dinitrophenols / Q000494:pharmacology; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals; D008928:Mitochondria / Q000201:enzymology / Q000378:metabolism*; D009097:Multienzyme Complexes / Q000378:metabolism*; D009840:Oligomycins / Q000494:pharmacology; D010084:Oxidation-Reduction; D010085:Oxidative Phosphorylation; D010101:Oxygen Consumption; D010710:Phosphates / Q000494:pharmacology; D010770:Phosphotransferases / Q000378:metabolism*
D004140:Dinitrophenols; D005609:Free Radicals; D009097:Multienzyme Complexes; D009840:Oligomycins; D010710:Phosphates; D000244:Adenosine Diphosphate; D010770:Phosphotransferases
Issledovanie mitokhondriĭ v osnovnykh metabolicheskikh sostoianiiakh pri razlichnykh urovniakh aktivnosti ATF-sintetazy metodom EPR.