A method of the rapid preparation of adenosine 5'-gamma-[32P] triphosphate by chemical synthesis.
A new chemical method for the synthesis of adenosine 5'-gamma-[32P] triphosphate has been developed based on the reaction of adenosine 5'-diphosphate with ethyl chloroformate. The resulting active mixed anhydride was able to react with [32P]-triethylammonium orthophosphate to give gamma-[32P]ATP.
D000255:Adenosine Triphosphate / Q000138:chemical synthesis* / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology; D007553:Isotope Labeling / Q000379:methods; D010761:Phosphorus Radioisotopes; D010766:Phosphorylation; D006656:Protamine Kinase / Q000378:metabolism
D010761:Phosphorus Radioisotopes; D000255:Adenosine Triphosphate; D006656:Protamine Kinase; D012321:DNA-Directed RNA Polymerases