Gas chromatographic-mass spectrometric determination of intact C3-hydroxylated benzodiazepine glucoronides in urine.
A method is described for the determination C3-hydroxylated benzodiazepine glucuronides in biological samples. Oxazepam and lorazepam glucuronides are measured as methyl esters and trimethylsilyl derivates by a gas chromatographic procedure. The applicability of the method has been tested on the urine of rats, guinea pigs, rabbits and man, receiving oxazepam orally. Oxazepam glucuronide was not found in rat urine but it was present in the urine of rabbits (5.7% of the administered dose), guinea pigs (9.5%) and man (13.4-26.9%).
D000284:Administration, Oral; D000818:Animals; D014151:Anti-Anxiety Agents / Q000652:urine*; D002849:Chromatography, Gas; D005965:Glucuronates / Q000652:urine; D006168:Guinea Pigs; D006801:Humans; D006900:Hydroxylation; D008140:Lorazepam / Q000008:administration & dosage / Q000652:urine*; D008297:Male; D013058:Mass Spectrometry; D010076:Oxazepam / Q000008:administration & dosage / Q000652:urine*; D011817:Rabbits; D051381:Rats; D014297:Trimethylsilyl Compounds / Q000652:urine
D014151:Anti-Anxiety Agents; D005965:Glucuronates; D014297:Trimethylsilyl Compounds; D010076:Oxazepam; D008140:Lorazepam