Four electrophoretic methods compared for diagnosis of type III hyperlipoproteinemia.
Blood drawn from 192 probands and 1129 first-degree relatives who were participants in a collaborative family study of hyperlipoproteinemia at nine Lipid Research Clinics was used to prepare aliquots of whole plasma and top (d less than 1.006 g/mL) and bottom (d greater than 1.006 g/mL) ultracentrifugal fractions. Each aliquot was analyzed at a central laboratory by electrophoresis on paper, agarose, and polyacrylamide gel, and by a combined electrophoretic precipitation technique. The electrophoretograms were evaluated for the presence or absence of a "floating-beta" lipoprotein band. All four methods agreed completely for 92.3% of the samples. An additional 2.0% of the samples were in agreement for three electrophoretic methods, but the paper electrophoretic results were not interpretable. Another 1.9% were considered to be "floating-beta" positive by paper electrophoresis but negative by the other three electrophoretic methods.
D011232:Chemical Precipitation; D004587:Electrophoresis, Agar Gel / Q000379:methods; D004590:Electrophoresis, Paper / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D005069:Evaluation Studies as Topic; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood / Q000175:diagnosis*; D008077:Lipoproteins, LDL / Q000097:blood*
D008077:Lipoproteins, LDL