Colloidal silica--aluminum modified--PVP density gradient centrifugation: centrifuge tube wall cell adherence, aggregation, separation properties and comparison to BSA and Ficoll.
A method is described for the inexpensive and easy preparation of colloidal silica--aluminum modified--polyvinylpyrrolidone (CS-AM-PVP) density gradient centrifugation medium. Using density gradient centrifugation, several cell separation and biochemical characteristics were studied: centrifuge tube wall cell adherence, mouse spleen cell density distribution, rebanding properties, mitogen response and cell aggregation. Cell adherence to the centrifuge tube wall using density gradients of CS-AM-PVP was compared with density gradients of bovine serum albumin and Ficoll. Few cells adhered to the centrifuge tube wall when CS-AM-PVP was used as a gradient medium; whereas, significant cell adherence to the centrifuge tube cell wall occurred when bovine serum albumin or Ficoll was used as a gradient medium. The CS-AM-PVP gradient medium did not inhibit the response to mitogens of mouse spleen cells which had been separated into density subpopulations in a discontinuous CS-AM-PVP density gradient, caused a minimum amount of cell aggregation, and was found to be non-toxic.
D000535:Aluminum; D000818:Animals; D002448:Cell Adhesion; D002449:Cell Aggregation; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D003102:Colloids; D005362:Ficoll; D006168:Guinea Pigs; D007942:Leukemia, Experimental; D012515:Mast-Cell Sarcoma / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D011134:Polysaccharides; D011205:Povidone; D012710:Serum Albumin, Bovine; D012822:Silicon Dioxide
D003102:Colloids; D011134:Polysaccharides; D005362:Ficoll; D012710:Serum Albumin, Bovine; D012822:Silicon Dioxide; D000535:Aluminum; D011205:Povidone