[Effect of synthetic somatostatin on basal and stimulated growth hormone and prolactin secretion in vitro].
The effect of synthetic somatostatin on the basal and stimulated secretion of growth hormone and prolactin under conditions of incubation of the adenohypophysis (ADH) tissue in vitro was studied. The labeled growth hormones (GH) and prolactin (Pl) secreted was assayed by the method of electrophoresis in polyacrylamide gel as modified by the authors. Synthetic somatostatin (10 and 100 nM) suppressed the basal and stimulated by 2--0-dibutyril AMP, theophylline, and prostaglandins of E series GH labeled secretion. In doses of 100 nM the inhibitor also suppressed the basal and the stimulated by 2--0-dibutyril AMP and theophylline release of labeled Pl. Apparently, the inhibitory action of somatostatin involves the reduction of the ADH activity of adenylatecyclase.
D000818:Animals; D003994:Bucladesine / Q000037:antagonists & inhibitors; D005260:Female; D013006:Growth Hormone / Q000378:metabolism*; D006728:Hormones / Q000494:pharmacology*; D066298:In Vitro Techniques; D010903:Pituitary Gland, Anterior / Q000378:metabolism*; D011388:Prolactin / Q000378:metabolism*; D011458:Prostaglandins E / Q000037:antagonists & inhibitors; D051381:Rats; D013004:Somatostatin / Q000494:pharmacology*; D013268:Stimulation, Chemical; D013806:Theophylline / Q000037:antagonists & inhibitors
D006728:Hormones; D011458:Prostaglandins E; D013004:Somatostatin; D003994:Bucladesine; D011388:Prolactin; D013006:Growth Hormone; D013806:Theophylline
Vliianie sinteticheskogo somatostatina na bazal'nuiu i stimuliruemuiu sekretsiiu gormona rosta i prolaktina in vitro.