Regulation of beta-adrenergic response in human lymphocytes: agonist induced subsensitivity.
The concentration of intracellular cAMP in lymphocytes is regulated by isoproterenol and PGE1. Preincubation of lymphocytes with isoproterenol leads to a loss of the response to beta-adrenergic agents but not to PGE1. Preincubation of lymphocytes with PGE1, however, affects the sensitivities both to isoproterenol and PGE1. A decrease of beta-adrenergic receptors was found in cells preincubated with isoproterenol but not with PGE1. Part of the abnormalities associated with PGE1-preincubated cells can be restored by treating the cells with theophylline.
D000242:Cyclic AMP / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007545:Isoproterenol / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects*; D011458:Prostaglandins E / Q000494:pharmacology; D011941:Receptors, Adrenergic / Q000187:drug effects*; D011943:Receptors, Adrenergic, beta / Q000187:drug effects*; D013806:Theophylline / Q000494:pharmacology
D011458:Prostaglandins E; D011941:Receptors, Adrenergic; D011943:Receptors, Adrenergic, beta; D013806:Theophylline; D000242:Cyclic AMP; D007545:Isoproterenol