The effect of pyrophosphate analogues on influenza virus RNA polymerase and influenza virus multiplication.
Analogues of pyrophosphate have been tested as inhibitors of influenza virus-RNA polymerase activity in cell-free assays. The most active compound, phosphonoformic acid (PFA), reduced the polymerase activity to 50 per cent at a concentration of 20 muM. The inhibition was dependent on the type of divalent cation present in the assay. PFA at a concentration of 400 muM also inhibited the influenza virus plaque formation by 90 per cent.
D012321:DNA-Directed RNA Polymerases / Q000037:antagonists & inhibitors*; D011756:Diphosphates / Q000494:pharmacology*; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D009975:Orthomyxoviridae / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development; D013329:Structure-Activity Relationship; D010948:Viral Plaque Assay
D011756:Diphosphates; D008345:Manganese; D012321:DNA-Directed RNA Polymerases; D008274:Magnesium