Purification and properties of gentisate 1,2-dioxygenase from Moraxella osloensis.
Gentisate:oxygen 1,2-oxidoreductase (decyclizing) (EC 1.13.11.4; gentisate 1,2-dioxygenase) from Moraxella osloensis was purified to homogeneity as shown by polyacrylamide gel electrophoresis. The enzyme has a molecular weight of about 154,000 and gives rise to subunits of molecular weight 40,000 in the presence of sodium dodecyl sulfate. Gentisate 1,2-dioxygenase showed broad substrate specificity and attacked a range of halogen- and alkyl-substituted gentisic acids. Maleylpyruvate, the product formed from gentisate, was degraded by cell extracts supplemented with reduced glutathione, but substituted maleylpyruvates were not attacked under these conditions.
D000645:Ammonium Sulfate; D002458:Cell Fractionation; D002474:Cell-Free System; D055598:Chemical Phenomena; D011232:Chemical Precipitation; D002621:Chemistry; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005841:Gentisates / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007535:Isomerases / Q000378:metabolism; D007700:Kinetics; D008970:Molecular Weight; D009016:Moraxella / Q000201:enzymology*; D010105:Oxygenases / Q000302:isolation & purification / Q000378:metabolism; D011773:Pyruvates / Q000096:biosynthesis; D012459:Salicylates / Q000378:metabolism; D012988:Soil Microbiology; D013056:Spectrophotometry, Ultraviolet
D005841:Gentisates; D011773:Pyruvates; D012459:Salicylates; D010105:Oxygenases; D007535:Isomerases; D005978:Glutathione; D000645:Ammonium Sulfate