Direct photoaffinity labeling of the primary region of the ouabain binding site of (Na+ + K+)-ATPase with [3H]ouabain, [3H]digitoxin and [3H]digitoxigenin.
The tritiated cardiotonic steroids, ouabain, digitoxin, and digitoxigenin are shown to photolabel the large polypeptide but not the glycoprotein or proteolipid component of the (Na+ + K+)-ATPase when they are bound to the inhibitory site and exposed to light of 220 or 254 nm. The extent of photolabeling is low, less than 1%, and is limited by photocross-linking of the enzyme. The mechanism of photoincorporation does not appear to be either photolysis of the lactone ring in ouabain or photolysis of tryptophan or tyrosine residues in the polypeptide.
D000345:Affinity Labels; D001665:Binding Sites; D004073:Digitoxigenin; D004074:Digitoxin; D007700:Kinetics; D010042:Ouabain; D010782:Photolysis; D011485:Protein Binding; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
D000345:Affinity Labels; D004073:Digitoxigenin; D010042:Ouabain; D004074:Digitoxin; D000254:Sodium-Potassium-Exchanging ATPase