Isolation and characterization of an adenovirus and isolation of its adenovirus-associated virus in cell culture from foals with respiratory tract disease.
An adenovirus was isolated from a foal with respiratory tract disease. The virus produced cytopathic effects (CPE) in equine embryo kidney (EEK) cell culture, contained deoxyribonucleic acid (DNA), was resistant to chloroform and pH 3, and was moderately resistant to heat. The virus caused hemagglutination of human (type O) erythrocytes. Viral density was 1.34 g/cm,3 and diameter was 75 nm. An adenovirus-associated virus (AAV) isolated from the infected cell culture was 22 nm in diameter. These viruses are classified as equine adenovirus and equine AAV.
D000256:Adenoviridae / Q000254:growth & development / Q000302:isolation & purification* / Q000648:ultrastructure; D000257:Adenoviridae Infections / Q000382:microbiology / Q000662:veterinary; D000818:Animals; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D003588:Cytopathogenic Effect, Viral; D004622:Embryo, Mammalian; D006386:Hemagglutination Tests; D006734:Horse Diseases / Q000382:microbiology*; D006736:Horses; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007668:Kidney; D012141:Respiratory Tract Infections / Q000382:microbiology / Q000662:veterinary*; D012526:Satellite Viruses / Q000254:growth & development / Q000302:isolation & purification* / Q000648:ultrastructure; D014779:Virus Replication