[Isolation of the plasma membranes of fat cells without using collagenase].
A method is described for isolation of plasmatic membranes of rat fatty cells immediately from fatty tissue without the treatment with collagenase. Homogenization of fatty tissue was carried out in large volumes of buffered sucrose and EDTA at room temperature followed by sucrose density gradient centrifugation. The preparations obtained exhibited high specific activity of the marker enzyme of plasmatic membranes [5'-nucleotidase and K+, Na+-ATPase], as well as high ability for specific binding of insulin.
D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D002462:Cell Membrane / Q000201:enzymology / Q000648:ultrastructure*; D002499:Centrifugation, Density Gradient; D004789:Enzyme Activation; D007328:Insulin / Q000378:metabolism; D008297:Male; D003012:Microbial Collagenase; D009708:Nucleotidases / Q000378:metabolism; D011485:Protein Binding; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013696:Temperature
D007328:Insulin; D009708:Nucleotidases; D003012:Microbial Collagenase; D000254:Sodium-Potassium-Exchanging ATPase
Vydelenie plazmaticheskikh membran zhirovykh kletok bez primeneniia kollagenazy.