title: Human gingival lymphocytes. I. Methodology for the isolation of human gingival lymphocytes.
abstract: Various methodologies were examined for the isolation of inflammatory cells from diseased human gingiva. The best recovery of viable gingival lymphocytes (gMNC) was achieved by a method which combined initial collagenase digestion followed by gentle teasing with an 18-gauge needle.
mesh_terms: D000293:Adolescent; D000328:Adult; D002469:Cell Separation / Q000379:methods*; D003094:Collagen / Q000032:analysis; D005881:Gingiva / Q000032:analysis / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D008214:Lymphocytes / Q000166:cytology*; D003012:Microbial Collagenase / Q000378:metabolism; D008875:Middle Aged; D010510:Periodontal Diseases / Q000473:pathology; D010950:Plasma Cells / Q000166:cytology
chemical_list: D003094:Collagen; D003012:Microbial Collagenase