Stimulation of capillary tube polymorph migration: an indirect glucocorticoid effect on microtubular function.
Polymorph migration stimulator (PMS) is a peptide factor produced by an in vitro reaction between glucocorticoids and human mononuclear phagocytes. This study was undertaken to determine the significance of the stimulatory effect of PMS on the capillary tube migration of human polymorphs. Colchicine, vinblastine and Nocodazole, all of which inhibit microtubular assembly, are shown to stimulate migration. Conversely, deuterium oxide which stabilizes microtubules inhibits migration. Increased intracellular cyclic AMP is associated with microtubular inhibition and isoprenaline, theophylline and dibutyryl cyclic AMP are also found to stimulate capillary tube migration. These results suggest that PMS acts by inhibiting the assembly of polymorph microtubules, an effect which may be mediated by cyclic AMP in the same manner as other peptide hormones.
D000249:Adenosine Monophosphate / Q000494:pharmacology; D001562:Benzimidazoles / Q000494:pharmacology; D002087:Butyrates / Q000494:pharmacology; D002217:Carbachol / Q000494:pharmacology; D002219:Carbamates / Q000494:pharmacology; D002634:Chemotaxis, Leukocyte / Q000187:drug effects*; D003078:Colchicine / Q000494:pharmacology; D000242:Cyclic AMP / Q000494:pharmacology; D003903:Deuterium / Q000494:pharmacology; D006801:Humans; D007545:Isoproterenol / Q000494:pharmacology; D007978:Levamisole / Q000494:pharmacology; D008870:Microtubules / Q000187:drug effects / Q000502:physiology*; D009504:Neutrophils / Q000502:physiology*; D010455:Peptides / Q000494:pharmacology*; D013806:Theophylline / Q000494:pharmacology; D014747:Vinblastine / Q000494:pharmacology
D001562:Benzimidazoles; D002087:Butyrates; D002219:Carbamates; D010455:Peptides; C001459:polymorph migration stimulator; D007978:Levamisole; D000249:Adenosine Monophosphate; D014747:Vinblastine; D002217:Carbachol; D003903:Deuterium; D013806:Theophylline; D000242:Cyclic AMP; D007545:Isoproterenol; D003078:Colchicine