An immunoperoxidase study on herpes simplex virus encephalitis.
Using formalin-fixed, paraffin-embedded sections, indirect immunoperoxidase studies were performed on 3 autopsied and 1 biopsied cases of herpes simplex virus (HSV) encephalitis. Specific reaction was observed in all 4 cases. Distinct stain was observed both in neurons and in glial cells. Electron microscopic studies performed on the same material revealed HSV particles stained with electron-dense reaction products. The advantages and disadvantages of the use of formalin-fixed, paraffin-embedded material in immunoperoxidase studies of viral disease are discussed.
D001921:Brain / Q000382:microbiology*; D002467:Cell Nucleus / Q000382:microbiology; D003593:Cytoplasm / Q000382:microbiology; D004660:Encephalitis / Q000209:etiology / Q000382:microbiology*; D006561:Herpes Simplex / Q000382:microbiology*; D006801:Humans; D007124:Immunoenzyme Techniques; D009457:Neuroglia / Q000382:microbiology; D009474:Neurons / Q000382:microbiology; D018139:Simplexvirus