title: Protease required for processing picornaviral coat protein resides in the viral replicase gene.
abstract: Partial purification of the encephalomyocarditis protease synthesized in extracts from rabbit reticulocytes shows that the activity responsible for cleaving coat precursor protein cosediments with a previously unmapped virus-coded protein with an apparent molecular weight of 20,000. Tryptic analysis shows that this protein is derived from protein D, a virus-coded component of the encephalomyocarditis RNA polymerase.
mesh_terms: D002213:Capsid / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D012321:DNA-Directed RNA Polymerases / Q000032:analysis* / Q000235:genetics; D004686:Encephalomyocarditis virus / Q000032:analysis / Q000201:enzymology* / Q000235:genetics; D005814:Genes, Viral; D010447:Peptide Hydrolases / Q000302:isolation & purification* / Q000378:metabolism; D011498:Protein Precursors / Q000378:metabolism; D014764:Viral Proteins / Q000032:analysis
chemical_list: D011498:Protein Precursors; D014764:Viral Proteins; D012321:DNA-Directed RNA Polymerases; D010447:Peptide Hydrolases