Tributyrate as a substrate for the determination of lipase activity in milk.
A procedure is described for the determination of the lipolytic activity in milk using tributyrate as substrate. The method is based on the titration of liberated butyric acid in a diethyl ether: light petroleum (2-75:1, v/v) extract of the incubation mixture. Some of the fundamental factors involved in the procedure are discussed.
D000818:Animals; D002087:Butyrates / Q000032:analysis / Q000378:metabolism; D002417:Cattle; D005019:Ethyl Ethers; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008049:Lipase / Q000032:analysis* / Q000378:metabolism; D008892:Milk / Q000201:enzymology*; D010578:Petroleum; D014280:Triglycerides / Q000378:metabolism
D002087:Butyrates; D005019:Ethyl Ethers; D010578:Petroleum; D014280:Triglycerides; D008049:Lipase