Human gingival lymphocytes. I. Methodology for the isolation of human gingival lymphocytes.
Various methodologies were examined for the isolation of inflammatory cells from diseased human gingiva. The best recovery of viable gingival lymphocytes (gMNC) was achieved by a method which combined initial collagenase digestion followed by gentle teasing with an 18-gauge needle.
D000293:Adolescent; D000328:Adult; D002469:Cell Separation / Q000379:methods*; D003094:Collagen / Q000032:analysis; D005881:Gingiva / Q000032:analysis / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D008214:Lymphocytes / Q000166:cytology*; D003012:Microbial Collagenase / Q000378:metabolism; D008875:Middle Aged; D010510:Periodontal Diseases / Q000473:pathology; D010950:Plasma Cells / Q000166:cytology
D003094:Collagen; D003012:Microbial Collagenase