Tissue differentiation and susceptibility to embryonal tumor induction by ethylnitrosourea in the opossum.
Opossums (Didelphis virginiana Kerr) exposed to 100 mg ENU/kg in single or incremental doses early in postnatal life developed a spectrum of epithelial and mesenchymal neoplasms including several types of embryonal neoplasms not previously induced in laboratory animals. A correlation was apparent to a varying degree between susceptibility to tumor induction and the state of morphologic maturation of the presumed target tissues at the light microscopic level for embryonal tumors of the eye, kidney, and brain. The susceptibility of the opossum eye to an ENU-induced intraocular teratoid medulloepithelioma extended over the period from 1 to between 3 and 4 weeks of age and was correlated with the differentiation of the apparent target cell, the nonpigmented ciliary epithelium of the pars ciliaris retinae. Induction of nephroblastomas was correlated with the presence in the kidney of stem cells (metanephric blastema) through the period from birth to between 6 and 8 weeks of age. Although susceptibility of the opossum brain to ENU induction of gangliogliomas was correlated with the state of differentiation of the germinal matrix from birth to 56 days of age, induction of these tumors was essentially limited to the 1st week postpartum. No definite correlation between vulnerability to tumor induction and tissue maturation was evident for a tumor of the jaw (ameloblastoma) with presumed origin from embryonic dental remnants. Our results indicated that the opossium early in postnatal life is a useful model for the induction and characterization of certain of the major dysontogenetic tumors, which have been difficult or impossible to reproduce in the traditional laboratory species.
D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D001932:Brain Neoplasms / Q000139:chemically induced; D005038:Ethylnitrosourea / Q000008:administration & dosage*; D005134:Eye Neoplasms / Q000139:chemically induced; D005260:Female; D007573:Jaw Neoplasms / Q000139:chemically induced; D007680:Kidney Neoplasms / Q000139:chemically induced; D008297:Male; D009374:Neoplasms, Experimental / Q000139:chemically induced; D009373:Neoplasms, Germ Cell and Embryonal / Q000139:chemically induced* / Q000196:embryology / Q000473:pathology; D009607:Nitrosourea Compounds / Q000008:administration & dosage*; D009808:Odontogenic Tumors / Q000139:chemically induced; D009893:Opossums
D009607:Nitrosourea Compounds; D005038:Ethylnitrosourea