Structural investigations of DNA-histone complexes. A spin label study.
We have prepared two acridine spin labels, 6-chloro-9-[4-(2,2,6,6-tetramethyl-1-piperidinyloxy)amino]-2-methoxyacridine (I) and 9-[4-(2,2,6,6-tetramethyl-1-piperidinyloxy)amino]-acridine (II) and have used them to study the binding of lysine-rich histone (H1) to DNA using electron spin resonance (ESR). ESR spectra of I in the presence of DNA, polydA-polydT and polydG-polydC were characteristic of highly immobilized radicals with maximum hyperfine splitting (2T11) of 59G, 62.5G and 59G respectively. However, the 2T11 values for II in the same systems were 55.5G, 55.5G and 62.5G respectively. Addition of H1 at a low P/D released ionically bound I and II from DNA. In the presence of 0.1 M NaCl, which prevents ionic binding, H1 still caused a significant release of bound II but not I from DNA. At a high P/D (with or without NaCl) H1 caused no displacement of either I or II. Our findings suggest that H1 does not affect the intercalating sites and probably binds to one of the grooves of DNA, most probably the major groove, and specifically in the A-T-rich regions.
D004247:DNA; D003852:Deoxyribonucleoproteins; D004578:Electron Spin Resonance Spectroscopy; D006657:Histones; D009690:Nucleic Acid Conformation; D009698:Nucleoproteins; D011485:Protein Binding; D011487:Protein Conformation; D013113:Spin Labels
D003852:Deoxyribonucleoproteins; D006657:Histones; D009698:Nucleoproteins; D013113:Spin Labels; D004247:DNA