title: Rat haemoglobin heterogeneity. Two structurally distinct alpha chains and functional behaviour of selected components.
abstract: Six haemoglobins were separated analytically from haemolysates of adult Wistar rats (Rattus norvegicus) by cellulose acetate electrophoresis and preparatively by DEAE-cellulose chromatography. The globin chains were separated from unfractionated haemolysates by CM-cellulose chromatography by using a non-linear formic acid-pyridine gradient followed by CM-cellulose chromatography in 8M-urea by using a gradient of increasing Na+ concentration in phosphate buffer, pH 6.7. Two alpha chains and three non-alpha chains were identified. Chains isolated from purified haemoglobins were correlated with chains isolated from unfractionated haemolysates by electrophoresis on urea-starch gels to make presumptive assignments of the subunit composition of the six haemoglobin tetramers. Partial amino acid sequences were determined for the major and minor alpha chains. The oxygen equilibria of two of the major haemoglobin components and of the unfractionated haemolysate were examined at pH 7.5 and 8.0. The two purified haemoglobins exhibited similar oxygen affinities; the haemolysate, however, had a lower oxygen affinity than either of the two purified haemoglobins. Both the haemolysate and the two haemoglobins showed an alkaline Bohr effect larger than that of human haemoglobin A.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002848:Chromatography, DEAE-Cellulose; D004588:Electrophoresis, Cellulose Acetate; D005914:Globins / Q000032:analysis; D006454:Hemoglobins / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D046911:Macromolecular Substances; D010100:Oxygen / Q000097:blood; D010446:Peptide Fragments / Q000032:analysis; D011485:Protein Binding; D051381:Rats / Q000097:blood*
chemical_list: D006454:Hemoglobins; D046911:Macromolecular Substances; D010446:Peptide Fragments; D005914:Globins; D010100:Oxygen