title: DNA sequence organization in the genomes of five marine invertebrates.
abstract: The arrangement of repetitive and non-repetitive sequence was studied in the genomic DNA of the oyster (Crassostrea virginica), the surf clam (Spisula solidissima), the horseshoe crab (Limulus polyphemus), a nemertean worm (Cerebratulus lacteus) and a jelly-fish (Aurelia aurita). Except for the jellyfish these animals belong to the protostomial branch of animal evolution, for which little information regarding DNA sequence organization has previously been available. The reassociation kinetics of short (250-300 nucleotide) and long (2,000-3,000 nucleotide) DNA fragments was studied by the hydroxyapatite method. It was shown that in each case a major fraction of the DNA consists of single copy sequences less than about 3,000 nucleotides in length, interspersed with short repetitive sequences. The lengths of the repetitive sequences were estimated by optical hyperchromicity and S1 nuclease measurements made on renaturation products. All the genomes studied include a prominent fraction of interspersed repetitive sequences about 300 nucleotides in length, as well as longer repetitive sequence regions.
mesh_terms: D000818:Animals; D001483:Base Sequence; D005075:Biological Evolution; D049872:Bivalvia; D003386:Brachyura; D002499:Centrifugation, Density Gradient; D002845:Chromatography; D003063:Cnidaria; D004247:DNA / Q000032:analysis*; D004277:DNA, Single-Stranded / Q000032:analysis; D003851:Deoxyribonucleases; D004586:Electrophoresis; D006882:Hydroxyapatites; D007448:Invertebrates; D009691:Nucleic Acid Denaturation; D009695:Nucleic Acid Renaturation; D009711:Nucleotides / Q000032:analysis*; D010125:Ostreidae; D010986:Platyhelminths
chemical_list: D004277:DNA, Single-Stranded; D006882:Hydroxyapatites; D009711:Nucleotides; D004247:DNA; D003851:Deoxyribonucleases