Endogenous digitalis-like activity in mammalian brain.
A fraction of brain has been prepared that contains a substance mimicking actions of the digitalis glycosides. It both blocks the binding of [3H]ouabain to Na+,K+-ATPase (ATP phosphohydrolase, EC 3.6.1.3) and inhibits the uptake of 86Rb+ into human erythrocytes. Partial isolation was achieved by Sephadex G-10 fractionation and subsequent desalting. This activity is potentially capable of regulating ionic fluxes and concentration gradients across the cell membrane.
D000818:Animals; D001667:Binding, Competitive; D001693:Biological Transport, Active / Q000187:drug effects; D001921:Brain / Q000502:physiology*; D004071:Digitalis Glycosides / Q000494:pharmacology*; D004910:Erythrocyte Membrane / Q000378:metabolism; D006168:Guinea Pigs; D006801:Humans; D008861:Microsomes / Q000378:metabolism; D010042:Ouabain / Q000037:antagonists & inhibitors; D012413:Rubidium / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors*
D004071:Digitalis Glycosides; D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase; D012413:Rubidium