title: Conversion of agroclavine to setoclavine and isosetoclavine in cell-free extracts from Claviceps sp. SD 58 and in a thioglycolate-iron (II) system.
abstract: Agroclavine was converted to setoclavine and isosetoclavine in crude extracts from Claviceps sp. SD 58. The ratio of setoclavine to isosetoclavine was 0.95. The conversion with boiled crude extract was 68% of the conversion with unboiled extract. In a thioglycolate-iron (II) system at 40 degrees for 5 hr, 45.5% of agroclavine was converted to setoclavine and isosetoclavine. At 70 degrees for 4 hr in the thioglycolate-iron (II) system, 4% of 4-dimethylallyltryptophan was converted to clavicipitic acid.
mesh_terms: D000470:Alkaloids / Q000302:isolation & purification / Q000378:metabolism*; D002250:Carbon Radioisotopes; D002411:Cation Exchange Resins; D002474:Cell-Free System; D002855:Chromatography, Thin Layer; D002967:Claviceps / Q000378:metabolism*; D003470:Culture Media; D005470:Fluorometry; D006863:Hydrogen-Ion Concentration; D007501:Iron; D007536:Isomerism; D013864:Thioglycolates; D014364:Tryptophan / Q000378:metabolism
chemical_list: D000470:Alkaloids; D002250:Carbon Radioisotopes; D002411:Cation Exchange Resins; D003470:Culture Media; D013864:Thioglycolates; D014364:Tryptophan; D007501:Iron