Synthesis of rabbit beta-globin in cultured monkey kidney cells following infection with a SV40 beta-globin recombinant genome.
Rabbit beta-globin complementary DNA (cDNA) has been inserted into SV40 DNA in place of the gene coding for the virus' major capsid protein, VP1. The recombinant genome, SVGT5-RabetaG, multiplies efficiently in CV1 monkey kidney cell cultures and is transcribed to yield cytoplasmic, polyadenylated hybrid mRNAs containing the beta-globin coding sequence. Cells propagating SVGT5-RabetaG produce substantial quantities of rabbit beta-globin polypeptide.
D000818:Animals; D002460:Cell Line; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004274:DNA, Recombinant; D005814:Genes, Viral; D005914:Globins / Q000235:genetics* / Q000378:metabolism; D008722:Methods; D009694:Nucleic Acid Precursors / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D013539:Simian virus 40 / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics
D004274:DNA, Recombinant; D009694:Nucleic Acid Precursors; D012333:RNA, Messenger; D014764:Viral Proteins; D005914:Globins; D004262:DNA Restriction Enzymes