Measurement of pyrimidine dimers in spheroplasts of Bacillus subtilis.
A method is described for making spheroplasts of Bacillus subtilis which are permeable to exogenous enzymes. Conditions are described for measuring small numbers of pyrimidine dimers in the DNA of UV-irradiated cells by use of a partially purified Micrococcus luteus extract containing an enzyme specific for pyrimidine dimers. The system will detect as few as 10-12 pyrimidine dimers per genome.
D001412:Bacillus subtilis / Q000032:analysis* / Q000528:radiation effects; D004269:DNA, Bacterial / Q000032:analysis* / Q000528:radiation effects; D004720:Endonucleases / Q000378:metabolism; D004926:Escherichia coli / Q000032:analysis / Q000528:radiation effects; D008837:Micrococcus / Q000201:enzymology; D011740:Pyrimidine Dimers / Q000032:analysis*; D013104:Spheroplasts / Q000032:analysis* / Q000528:radiation effects; D014466:Ultraviolet Rays
D004269:DNA, Bacterial; D011740:Pyrimidine Dimers; D004720:Endonucleases