Determination of cholesterol and phospholipid concentrations in high-density lipoproteins by heparin-manganese chloride precipitation.
Cholesterol as well as phospholipids in high-density lipoproteins (HDL) have been measured in 60 serum samples after heparin-manganese chloride precipitation of the apolipoprotein B containing lipoproteins. The values obtained correlated well with values from ultracentrifugal separation (slope 1.11 for cholesterol, 0.92 for phospholipids). Furthermore, there was a significant correlation between the low-density lipoprotein (LDL) cholesterol estimated by the formula of Friedewald et al. and the ultracentrifugally separated LDL cholesterol (slope 1.02). By the additional determination of HDL phospholipids with a precipitation procedure, further information about the "antiatherogenic" is easily available.
D000328:Adult; D011232:Chemical Precipitation; D002784:Cholesterol / Q000097:blood*; D006493:Heparin; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood*; D008077:Lipoproteins, LDL / Q000097:blood; D008345:Manganese; D008875:Middle Aged; D010743:Phospholipids / Q000097:blood*; D014461:Ultracentrifugation
D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D010743:Phospholipids; D008345:Manganese; D006493:Heparin; D002784:Cholesterol