Influence of the ionic composition of the medium on the sensitivity of fertilized sea urchin ova to neuropharmacological preparations.
The removal of Ca2+ from the incubation medium leads to an increase in the sensitivity of early sea urchin embryos to neuropharmacological blockers of development. The removal of SO4-2-, half of the K+, as well as an increase in the Ca2+ content, have no effect on the sensitivity of fertilized eggs to these preparations. The sensitivity to puromycin, colchicine, triton X-100, and 2, 4-dinitrophenol is unchanged by the removal of Ca2+ from the medium. The protective action of exogenous monoamines and acetylcholine is not manifested in a solution that does not contain Ca2+. Serotonin, catecholamines, and acetylcholine have no appreciable effect on cleavage either in sea water of normal composition or in water without Ca2+.
D000109:Acetylcholine / Q000494:pharmacology; D000317:Adrenergic alpha-Antagonists / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000502:physiology; D002395:Catecholamines / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002463:Cell Membrane Permeability / Q000187:drug effects; D003078:Colchicine / Q000494:pharmacology; D003470:Culture Media; D003902:Detergents / Q000494:pharmacology; D004140:Dinitrophenols / Q000494:pharmacology; D005260:Female; D005306:Fertilization; D006863:Hydrogen-Ion Concentration; D010063:Ovum / Q000187:drug effects*; D010276:Parasympatholytics / Q000494:pharmacology*; D011691:Puromycin / Q000494:pharmacology; D012617:Sea Urchins / Q000196:embryology*; D012701:Serotonin / Q000494:pharmacology; D012702:Serotonin Antagonists
D000317:Adrenergic alpha-Antagonists; D002395:Catecholamines; D003470:Culture Media; D003902:Detergents; D004140:Dinitrophenols; D010276:Parasympatholytics; D012702:Serotonin Antagonists; D012701:Serotonin; D011691:Puromycin; D000109:Acetylcholine; D003078:Colchicine; D002118:Calcium