A microplaque reduction assay for human and mouse interferon.
A microplaque reduction assay for human and mouse interferons is described, using plastic disposable multicompartmental plates with flat-bottomed wells of 6 mm in diameter. The procedure is rapid and reproducible and lends itself to a considerable degree of automation. Its potential application in other areas of virology, such as rapid screening of samples for virus content or assay of neutralizing antibodies, is also discussed.
D002460:Cell Line; D003470:Culture Media; D003588:Cytopathogenic Effect, Viral; D005069:Evaluation Studies as Topic; D006863:Hydrogen-Ion Concentration; D007372:Interferons / Q000032:analysis* / Q000494:pharmacology; D007739:L Cells; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects / Q000254:growth & development; D010948:Viral Plaque Assay / Q000379:methods*
D003470:Culture Media; D007372:Interferons