Inhibition of 80S initiation complex formation by infection with poliovirus.
Anisomycin has been shown to stabilize ribosome initiation complexes containing messenger RNA and met-tRNAf met to high salt conditions. Extracts from HeLa cells treated with 5 X 10(-7) M-anisomycin for 15 min accumulate 80S initiation complexes which can be detected by their absorbance in sucrose gradients. Poliovirus-infected cells fail to form the 80S initiation complex early after infection, when inhibition of host cell protein synthesis occurs. These complexes re-form later in infection after virus RNA is synthesized. No re-formation occurs in the absence of virus replication. Thus, the step in protein synthesis inhibited by poliovirus precedes the entry of components into the 80S initiation complex.
D000841:Anisomycin / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D008715:Methionine; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D010442:Peptide Chain Initiation, Translational / Q000187:drug effects; D017955:Poliovirus / Q000254:growth & development*; D012333:RNA, Messenger / Q000378:metabolism; D012334:RNA, Neoplasm / Q000378:metabolism; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism; D014779:Virus Replication
D009363:Neoplasm Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D012346:RNA, Transfer, Amino Acyl; D000841:Anisomycin; D008715:Methionine