Potential-dependent conductances in lipid membranes containing alamethicin.
This article is concerned primarily with the mechanism of the potential-dependent conductance induced in artificial lipid membranes by the cyclic polypeptide andibiotic alamethicin. It has already been shown from studies of the fluctuations that can be detected in very small membrane currents that alamethicin forms transient pores of some 0.6 nm in diameter and that, for small inorganic ions, these are poorly selective. The origin of these pores, their spatial distribution and interaction are discussed. It is demonstrated that the sensitivity of the membrane conductance to the applied potential arises only to a slight extent from the current-voltage relations for the individual pores, and that the main effect stems from the influence of the potential on the frequency of opening of the pores. From the properties of lipid membranes containing alamethicin in a wide variety of electrolytes, and from other evidence, it is concluded that the polypeptide reacts to the electric field more probably because it has dipole moment than because it binds ions. It is proposed that the conducting complex is capable of functioning in either of two orientations, and that it is these two possibilities that give rise to certain differences in the single channel characteristics for the two directions of the field.
D000408:Alamethicin; D000900:Anti-Bacterial Agents; D002414:Cations, Monovalent; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004594:Electrophysiology; D005071:Evoked Potentials; D008055:Lipids; D008564:Membrane Potentials; D008567:Membranes, Artificial; D008959:Models, Neurological; D009431:Neural Conduction; D013499:Surface Properties
D000900:Anti-Bacterial Agents; D002414:Cations, Monovalent; D008055:Lipids; D008567:Membranes, Artificial; D000408:Alamethicin