Anaphylactic relase of a basophil kallikrein-like activity. II. A mediator of immediate hypersensitivity reactions.
This report describes the immune release of a new mediator from human peripheral leukocytes, a basophil kallikrein-like activity (BK-A). The release process is initiated by the interaction of antigen on anti-IgE with cell-bound IgE, and appears to be similar in mechanism to the relase of histamine and other mediators of the immediate hypersensitivity reaction. The dose-response relationships and kinetics of histamine and BK-A release from antigen-challenged peripheral leukocytes are similar. The relase of the BK-A is calcium and temperature dependent, requires metabolic energy, and is controlled by hormone-receptor interactions that influence the cellular level of cyclic AMP, as has been described for other mediators of immediate hypersensitivity reactions. The data indicate that the interaction of BK-A with human plasma kininogen, generates immunoreactive kinin. We conclude that the antigen-IgE interation leads to the release from human basophils of a new mediator, a basophil kallikrein-like activity which may well be a link between reactions of immediate hypersenstivitity and the plasma and/or tissue kinin-generating systems.
D000707:Anaphylaxis / Q000097:blood / Q000201:enzymology*; D000906:Antibodies; D001120:Arginine; D001491:Basophils / Q000201:enzymology*; D003994:Bucladesine / Q000494:pharmacology; D000001:Calcimycin / Q000494:pharmacology; D003078:Colchicine / Q000494:pharmacology; D003847:Deoxyglucose / Q000494:pharmacology; D003903:Deuterium / Q000494:pharmacology; D004950:Esterases / Q000378:metabolism; D006632:Histamine / Q000097:blood; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000097:blood / Q000201:enzymology* / Q000209:etiology; D007073:Immunoglobulin E / Q000276:immunology; D066298:In Vitro Techniques; D007610:Kallikreins / Q000378:metabolism*; D007700:Kinetics; D007962:Leukocytes / Q000201:enzymology; D008214:Lymphocytes / Q000201:enzymology; D013696:Temperature; D013806:Theophylline / Q000494:pharmacology
D000906:Antibodies; D007073:Immunoglobulin E; D000001:Calcimycin; D003994:Bucladesine; D006632:Histamine; D001120:Arginine; D003847:Deoxyglucose; D003903:Deuterium; D013806:Theophylline; D004950:Esterases; D007610:Kallikreins; D003078:Colchicine