The characterization of phosphoseryl tRNA from lactating bovine mammary gland.
BD-cellulose and RPC-5 chromatography of tRNA isolated from lactating bovine mammary gland showed the presence of four seryl-tRNA isoacceptors. The species, tRNA IV Ser, with the strongest affinity for BD-cellulose (required ethanol in the elution buffer) could be phosphorylated in the presence of serine, [gamma-32 P]-ATP, seryl-tRNA synthetase and phosphotransferase activity from the same tissue. O-Phosphoserine was identified as the 32P-labelled product after mild alkaline hydrolysis of this aminoacylated tRNA. Pancreatic ribonuclease treatment of the aminoacylated tRNA yielded a labelled product which was identified as phosphoseryladenosine. These results indicated there is a specific phosphoseryl tRNA species in lactating bovine mammary gland. It appears that the formation of phosphoseryl-tRNA proceeds by enzymic phosphorylation of seryl-tRNA.
D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism; D000818:Animals; D002417:Cattle; D002852:Chromatography, Ion Exchange; D005260:Female; D007774:Lactation; D008321:Mammary Glands, Animal / Q000378:metabolism*; D009943:Organophosphorus Compounds; D010770:Phosphotransferases / Q000378:metabolism; D011247:Pregnancy; D012346:RNA, Transfer, Amino Acyl / Q000096:biosynthesis / Q000378:metabolism; D012694:Serine / Q000031:analogs & derivatives
D009943:Organophosphorus Compounds; D012346:RNA, Transfer, Amino Acyl; D012694:Serine; D010770:Phosphotransferases; D000604:Amino Acyl-tRNA Synthetases