title: T4 DNA topoisomerase: a new ATP-dependent enzyme essential for initiation of T4 bacteriophage DNA replication.
abstract: A novel ATP-dependent DNA topoisomerase which makes reversible double-strand breaks in the DNA double helix has been purified to near homogeneity from T4 bacteriophage-infected Escherichia coli cells. Genetic data suggest that this activity is essential for initiating T4 DNA replication forks in vivo.
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D004261:DNA Replication; D004264:DNA Topoisomerases, Type I / Q000302:isolation & purification / Q000378:metabolism*; D004278:DNA, Superhelical / Q000378:metabolism; D005796:Genes; D005814:Genes, Viral; D009154:Mutation; D012313:RNA / Q000378:metabolism; D013604:T-Phages / Q000201:enzymology / Q000235:genetics*; D014779:Virus Replication
chemical_list: D004278:DNA, Superhelical; D012313:RNA; D000255:Adenosine Triphosphate; D004264:DNA Topoisomerases, Type I