[Enzyme induction by Epstein-Barr-virus-producing infection of lymphoblastoid cell lines].
By means of biological in vitro assay collagenolytic activity of normal peripheral lymphocytes was compared to that of EBV-infected lymphoblastoid cell lines: the EBV-producing P3HR1 cells and the non-productive Raji cells. While normal lymphocytes and Raji cells revealed only traces of activity, significantly increased (p less than 0.001) collagenolytic activity was detected in samples of P3HR1, the cell-free EBV-suspension alone showed no collagenolysis. The described enzymatic activity was fully inhibited by 0-phenanthroline and EDTA indicating collagenase action. As main explanations for the phenomenon we suggest the possibility of viral enzyme induction, increased release of protease and thirdly that EBV carries a gene for synthesis of a collagenase.
D002051:Burkitt Lymphoma / Q000201:enzymology*; D002460:Cell Line; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D004789:Enzyme Activation; D004790:Enzyme Induction; D005260:Female; D004854:Herpesvirus 4, Human / Q000201:enzymology; D006801:Humans; D008214:Lymphocytes / Q000201:enzymology; D003012:Microbial Collagenase / Q000096:biosynthesis*
D003094:Collagen; D003012:Microbial Collagenase
Enzyminduktion durch produzierende Infektion von lymphoblastoiden Zellinien durh Epstein-Barr-Virus.