Pepsin immobilized by covalent fixation to hydroxyalkyl methacrylate gels: preparation and characterization.
Insoluble active derivatives of pepsin (EC 3.4.23.1) were prepared by covalent binding of this enzyme to hydroxyalkyl methacrylate gels modified with 1,6-diaminohexane or epsilon-aminocaproic acid in an acid medium by means of water-soluble carbodiimide. The amount of attached enzyme, its proteolytic activity, pH activity curves of the preparations obtained and the time and pH dependence of their stability were determined.
D004355:Drug Stability; D005782:Gels; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008689:Methacrylates; D010434:Pepsin A / Q000378:metabolism*; D011485:Protein Binding; D012995:Solubility
D005782:Gels; D008689:Methacrylates; D010434:Pepsin A