Kinetics of 5--nucleotidase in sera of liver cirrhotic and normal Iraqi individuals.
5'-Nucleotidase activity was elevated in patients with liver cirrhosis; greater values of 5'-Nucleotidase activity were found in biliary cirrhosis, 5'-Nucleotidase from liver cirrhotic sera was less stable than from normal sera. The velocity of 5'Nucleotidase from liver cirrhotic sera per minute, at t = 10, was greater than normal controls. The optimum (S) for 5'-Nucleotidase was found to be 1.0 mM A-5'-MP, for both normal and liver cirrhotic sera. Km (A-5'-MP) and (2'-d-A-5'-MP) of 5'-Nucleotidase was found to be significantly lower in patients with liver cirrhosis than normal controls.
D015720:5'-Nucleotidase; D000328:Adult; D005260:Female; D006801:Humans; D007493:Iraq; D007700:Kinetics; D008103:Liver Cirrhosis / Q000201:enzymology*; D008297:Male; D009708:Nucleotidases / Q000097:blood*; D012737:Sex Factors; D013696:Temperature
D009708:Nucleotidases; D015720:5'-Nucleotidase