Inhibition of K+-sensitive p-nitrophenylphosphatase activity on Rhesus-positive red cells after incubation with IgG-anti-Rhesus-D.
The incubation of ghosts derived from human Rhesus-positive red cells with IgG-anti-Rhesus-D inhibited the K+-sensitive p-nitrophenylphosphatase activity. This enzyme has a partial function in the (Na+ + K+)-ATPase system related to the phosphorylation step, which is important for active potassium transport through the red cell membrane. The specificity of the impairment by the antigen-antibody reaction in the Rhesus-D system was proved by the following controls. Ghosts obtained from Rhesus-negative red cells incubated by IgG-anti-Rhesus-D and those of Rhesus-positive red cells treated with non-immune serum did not show any reduction of the K+-p-nitrophenylphosphatase activity. The ghost preparation with lanthanum carried out after hypotonic hemolysis of the washed red cells in 2 mM LaCl3 at pH 6 was the most suitable procedure to explore this topic in comparison to other techniques for preparing ghosts of red cells.
D009597:4-Nitrophenylphosphatase / Q000097:blood* / Q000276:immunology; D000937:Antigen-Antibody Reactions; D001693:Biological Transport, Active; D004910:Erythrocyte Membrane / Q000276:immunology / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D006461:Hemolysis; D006801:Humans; D007074:Immunoglobulin G; D010744:Phosphoric Monoester Hydrolases / Q000097:blood*; D011188:Potassium / Q000097:blood*; D012204:Rh-Hr Blood-Group System; D000254:Sodium-Potassium-Exchanging ATPase / Q000097:blood
D007074:Immunoglobulin G; D012204:Rh-Hr Blood-Group System; D010744:Phosphoric Monoester Hydrolases; D009597:4-Nitrophenylphosphatase; D000254:Sodium-Potassium-Exchanging ATPase; D011188:Potassium