title: Effect of bile-duct ligation on organelle marker enzymes in the liver and serum of rats.
abstract: 1. Marker enzymes for the principal subcellular organelles of rat liver were asayed in the liver of rats 1 day and 8 days after bile-duct ligation or after laparotomy as a control procedure. 2. The microsomal enzymes in liver tissue showed complex changes. Benz[alpha]pyrene hydroxylase activity, predominantly found in the smooth endoplasmic reticulum, was decreased. Glucose 6-phosphatase activity and ribonucleic acid, which are localized predominantly in the rough endoplasmic reticulum, were increased. 3. The plasma membrane enzyme, alkaline phosphatase, increased in activity after bile-duct ligation. 4. No changes in mitochondrial enzyme activities were noted after 1 day but there was a 50% reduction 8 days after ligation. Lysosomal enzyme activities did not change in the liver tissue. 5. Liver catalase and D-amino acid oxidase activities showed a slight increase at 1 day postligation but a significant fall by 8 days. 6. Lactate dehydrogenase, a cytosol enzyme, showed a decrease in activity after 1 day but an increase in tissue activities 8 days after ligation. 7. Serum activities of mitochondrial, plasma membrane, microsomal, lysosomal and cytosol marker enzymes tended to increase post-ligation, particularly at 8 days. 8. Monoamine oxidase, a predominantly mitochondrial enzyme, was greatly elevated in the serum after 1 day but had returned to normal activities by 8 days.
mesh_terms: D000118:Acetylglucosaminidase / Q000378:metabolism; D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001579:Benzopyrene Hydroxylase / Q000378:metabolism; D001652:Bile Ducts / Q000502:physiology*; D002374:Catalase / Q000378:metabolism; D002462:Cell Membrane / Q000201:enzymology; D003600:Cytosol / Q000201:enzymology; D003605:D-Amino-Acid Oxidase / Q000378:metabolism; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D005959:Glucosidases / Q000378:metabolism; D005966:Glucuronidase / Q000378:metabolism; D006596:Hexosaminidases / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008026:Ligation; D008099:Liver / Q000201:enzymology*; D008247:Lysosomes / Q000201:enzymology; D008291:Malate Dehydrogenase / Q000378:metabolism; D008830:Microbodies / Q000201:enzymology; D008862:Microsomes, Liver / Q000201:enzymology; D008928:Mitochondria / Q000201:enzymology; D008995:Monoamine Oxidase / Q000378:metabolism; D051381:Rats; D013347:Subcellular Fractions / Q000201:enzymology
chemical_list: D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D002374:Catalase; D001579:Benzopyrene Hydroxylase; D003605:D-Amino-Acid Oxidase; D008995:Monoamine Oxidase; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D005952:Glucose-6-Phosphatase; D005959:Glucosidases; D006596:Hexosaminidases; D005966:Glucuronidase; D000118:Acetylglucosaminidase