Subcellular distribution and properties of kynurenine pyruvate transaminase in rat kidney.
Kynurenine transaminase activity in rat kidney was found in both the mitochondrial and supernatant fractions. These fractions contained (a) kynurenine pyruvate transaminase, which showed a preference for pyruvate as amino acceptor, and had a pH optimum between 8.0 and 8.5, and (b) kynurenine 2-oxoglutarate transaminase, with a preference for 2-oxoglutarate and a pH optimum between 6.0 and 6.5. The apparent Km value of the former enzyme for L-kynurenine was much lower than that of the latter enzyme.
D003600:Cytosol / Q000201:enzymology; D006863:Hydrogen-Ion Concentration; D007656:Ketoglutaric Acids; D007668:Kidney / Q000201:enzymology*; D007700:Kinetics; D007737:Kynurenine; D008930:Mitochondria, Liver / Q000201:enzymology; D011773:Pyruvates; D013347:Subcellular Fractions / Q000201:enzymology; D000637:Transaminases / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*
D007656:Ketoglutaric Acids; D011773:Pyruvates; D007737:Kynurenine; D000637:Transaminases