title: Dibenamine blockade on gastric acid secretion in vitro and its protection by histamine antagonists.
abstract: Experiments to protect the histamine receptor against dibenamine blockade were carried out to elucidate the pharmacological characteristics of the H2-histamine receptor system for gastric acid secretion in isolated bullfrog gastric mucosa. Dibenamine alone (5 times 10-minus 5 g/ml) irreversibly blocked both basal and histamine-stimulated acid secretion. However, when the preparation was treated with dibenamine in combination with histamine (1 times 10-minus 5 g/ml) irreversibly blocked both basal and histamine-stimulated acid secretion. However, when the preparation was treated with dibenamine in combination with histamine (1 times 10-minus 5 g/ml), the acid secretory response to histamine was restored after washing out dibenamine. Burimamide, an H2-receptor antagonist, also protected the histamine sensitivity against dibenamine blockade in the concentration of 1 times 10-minus 4 g/ml. Diphenhydramine and pyribenzamine were also protected with histamine receptor against dibenamine blockade. The acid secretion induced by the action of histamine on the diphenhydramine-protected receptor was antagonized by diphenhydramine as well as burimamide.
mesh_terms: D000818:Animals; D002049:Burimamide / Q000494:pharmacology; D003864:Depression, Chemical; D003983:Dibenzylchlorethamine / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D004155:Diphenhydramine / Q000494:pharmacology; D005750:Gastric Juice / Q000378:metabolism*; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism*; D006634:Histamine H1 Antagonists / Q000494:pharmacology*; D066298:In Vitro Techniques; D011892:Rana catesbeiana; D011955:Receptors, Drug
chemical_list: D006634:Histamine H1 Antagonists; D011955:Receptors, Drug; D003983:Dibenzylchlorethamine; D004155:Diphenhydramine; D002049:Burimamide