Detection and changes of nucleotide pyrophosphotransferase activity of Streptomyces griseus strains in submerged culture.
Nucleotide pyrophosphotransferase (NPT) activity of two Streptomyces griseus strains was studied in submerged culture during their life cycle. NPT activity could be detected only in the culture filtrate but not in the membrane fraction or in cell extract of the sporulating (streptomycin-non-producing) S. griseus No. 45-H. No enzyme could be detected in the non-sporulating (streptomycin-producing) S. griseus No 52--1 cultures.
D002478:Cells, Cultured; D017854:Diphosphotransferases; D009711:Nucleotides / Q000378:metabolism; D010770:Phosphotransferases / Q000378:metabolism*; D013305:Streptomyces griseus / Q000201:enzymology*; D013379:Substrate Specificity
D009711:Nucleotides; D010770:Phosphotransferases; D017854:Diphosphotransferases; C021900:nucleotide pyrophosphokinase