Acid glycohydrolase in Chinese hamster (Cricetulus griseus) with spontaneous diabetes--V. Subcellular distribution in the kidney.
1. Seven renal glycohydrolases were measured in four subcellular fractions prepared from highly inbred aglycosuric (AV-line) and glycosuric (XA-line) Chinese hamsters. 2. alpha-D-galactosidase and beta-D-galactosidase were highest in the nuclear (N) and supernatant (S) fractions; both fractions showed reduced activities in the XA animals. 3. alpha-D-mannosidase was chiefly a particulate enzyme and its decrease in XA animals was evident in N, lysosomal-mitochondrial (LM) and mitochondrial-microsomal (MM) fractions. 4. No significant difference in N-acetyl-beta-D-glucosaminidase was found in any of these subcellular fractions between AV and XA animals. 5. Although total alpha-L-fucosidase and beta-D-fucosidase levels were similar in AV and XA kidneys, a difference was observed in the S fraction. 6. beta-D-glucuronidase was virtually absent in N and LM fractions and the S fraction of AV kidneys showed higher activity than the XAs.
D000118:Acetylglucosaminidase / Q000378:metabolism; D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology; D006224:Cricetinae; D003412:Cricetulus; D003593:Cytoplasm / Q000201:enzymology; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology*; D005260:Female; D005966:Glucuronidase / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology* / Q000648:ultrastructure; D008247:Lysosomes / Q000201:enzymology; D008361:Mannosidases / Q000378:metabolism; D008861:Microsomes / Q000201:enzymology; D008928:Mitochondria / Q000201:enzymology; D000519:alpha-Galactosidase / Q000378:metabolism; D005644:alpha-L-Fucosidase / Q000378:metabolism; D043323:alpha-Mannosidase; D001616:beta-Galactosidase / Q000378:metabolism
D006026:Glycoside Hydrolases; D008361:Mannosidases; D000519:alpha-Galactosidase; D001616:beta-Galactosidase; D043323:alpha-Mannosidase; D005966:Glucuronidase; C028219:beta-D-fucosidase; D005644:alpha-L-Fucosidase; D000118:Acetylglucosaminidase