A rapid method, using octadecasilyl-silica, for the extraction of certain peptides from tissues.
Peptides can be recovered from tissues by homogenization in a carefully selected extraction medium followed by adsorption from the supernatant on a small bed of octadecasilyl-silica. Recoveries of corticotropins and somatostatin added to a variety of tissues were quantitative, and the peptides undamaged as determined by high-pressure liquid chromatography.
D000324:Adrenocorticotropic Hormone / Q000302:isolation & purification; D000818:Animals; D008297:Male; D008722:Methods; D010455:Peptides / Q000302:isolation & purification*; D051381:Rats; D012822:Silicon Dioxide; D013004:Somatostatin / Q000302:isolation & purification
D010455:Peptides; C026679:octadecylsilica; D013004:Somatostatin; D012822:Silicon Dioxide; D000324:Adrenocorticotropic Hormone