The structure of Klebsiella serotype II capsular polysaccharide.
Using periodate oxidation, methylation analysis, the characterization of oligosaccharides obtained by partial acid hydrolysis, p.m.r. spectroscopy, and analytical ultracentrifugation, the structure of the (mildly alkali-treated) Klebsiella serotype 11 capusular polysaccharide has been elucidated. The tetrasaccharide repeating-unit comprises the sequence yields 3)-beta-D-Glcp-(1 yields 3)-beta-D-GlcUAp-(1 yields 3)-alpha-D-Galp-(1 yields with a 4,6-O-(1-carboxyethylidene)-alpha-D-galactosyl residue linked to O-4 of the glucuronic acid residue. The structural basis for some serological cross-reactions of the Klebseilla K11 antigen is discussed, and it is shown that rabbit antisera against the Klebsiella K11 test-strain predominantly contain K agglutinins specific for branch-terminal 4,6-O-(1-carboxyethylidene)-D-galactose.
D000818:Animals; D003429:Cross Reactions; D004926:Escherichia coli / Q000276:immunology; D005690:Galactose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D005965:Glucuronates / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D005779:Immunodiffusion; D007709:Klebsiella / Q000032:analysis* / Q000276:immunology; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008968:Molecular Conformation; D008970:Molecular Weight; D010084:Oxidation-Reduction; D010504:Periodic Acid; D011135:Polysaccharides, Bacterial / Q000032:analysis; D011817:Rabbits / Q000276:immunology; D014461:Ultracentrifugation
D005965:Glucuronates; D011135:Polysaccharides, Bacterial; D010504:Periodic Acid; D005947:Glucose; D005690:Galactose