title: Analysis of the electron spin resonance spectrum of human liver tissue embedded in paraffin.
abstract: The ESR spectra of the liver samples embedded in paraffin of newborn, adult, cirrhotic and control individuals always display the g approximately 2.05 signal and occasionally also the g = 4.3 line and the signal of free radicals. In adults, the amplitude of the g approximately 2.05 signal is negatively correlated with that of the g approximately 4.3 signal; in infants aged from three months to one year this correlation is positive. The copper content of the liver is in linear correlation with the amplitude of the g approximately 2.05 signal. The hyperfine structure of the part of the g approximately 2.05 signal recorded at low magnetic field may be of three types: it may correspond to Cu2+ centers of axial symmetry of type I, II or I + II. The parameters of the signal are the following: type I center, g = 2.35 +/- 0.05, g = 2.05 +/- 0.01, A < 100 gauss; type II center, g = 2.25 +/- 0.03, g = 2.05 +/- 0.01, A = 160 +/- 20 gauss. On the basis of the amplitude of the g approximately 2.05 signal, the copper content of the liver tissue can be estimated from paraffin blocks with an error of 30%, without losing the tissue embedded for histological examination.
mesh_terms: D000293:Adolescent; D000328:Adult; D000367:Age Factors; D002648:Child; D003300:Copper / Q000032:analysis; D004578:Electron Spin Resonance Spectroscopy; D006652:Histological Techniques; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007234:Infant, Premature; D008099:Liver / Q000032:analysis*; D008103:Liver Cirrhosis / Q000378:metabolism; D008875:Middle Aged; D010232:Paraffin
chemical_list: D003300:Copper; D010232:Paraffin