Relationship between prostaglandins and cyclic-AMP in the process of implantation in rats.
The effect of alloxan, a known inhibitor of adenylate cyclase, on the levels of prostaglandins E and F was investigated in pregnant rats. Animals were treated intraluminally with alloxan in normal saline or alloxan plus cyclic-AMP in normal saline or normal saline alone on day 4 of pregnancy. The uteri were collected on days 5 and 6 of pregnancy and the prostaglandins E and F were extracted and measured by a radioimmunoassay technique. In the alloxan treated rats, a significant reduction in PGE and PGF levels was found on days 5 and 6 of pregnancy as compared to untreated control group, whereas in the alloxan plus cyclic-AMP treated group the levels of PGE and PGF were comparable to those of the untreated control group. These findings suggest a possible relationship between prostaglandins and cyclic-AMP in the process of implantation in rats.
D000496:Alloxan / Q000494:pharmacology; D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism / Q000494:pharmacology*; D010064:Embryo Implantation / Q000187:drug effects*; D005260:Female; D005865:Gestational Age; D011247:Pregnancy; D011458:Prostaglandins E / Q000378:metabolism*; D011460:Prostaglandins F / Q000378:metabolism*; D051381:Rats; D014599:Uterus / Q000187:drug effects / Q000378:metabolism
D011458:Prostaglandins E; D011460:Prostaglandins F; D000496:Alloxan; D000242:Cyclic AMP