Tumor-specific DNA sequences in human gliomas.
Utilizing the technique of hydroxyapatite chromatography, normal cellular DNAs were used to recycle off the repeat or normal sequences found in [3H]DNA copied off 70S RNA from malignant astrocytomas. The recycled [3H]DNA were then used to hybridize against DNAs from normal human brain tissues and DNAs from malignant astrocytomas or Grade IV astrocytomas. The results indicated the presence of tumor-specific DNA sequences in malignant astrocytomas, absent in normal brain tissues. The percentages were 88% and 7%, respectively. When recycled medulloblastoma 70S[3H]DNA probes were utilized against DNA'S FROM Grade IV astrocytomas and from normal brain, similar results were obtained. The respective percentages of hybridization were 67% and 7%. Thus it would appear that malignant gliomas contain tumor-specific DNA sequences which are not found in normal brain tissues.
D001254:Astrocytoma / Q000032:analysis; D001483:Base Sequence; D001932:Brain Neoplasms / Q000032:analysis*; D004273:DNA, Neoplasm / Q000302:isolation & purification*; D005909:Glioblastoma / Q000032:analysis; D005910:Glioma / Q000032:analysis*; D006801:Humans; D009693:Nucleic Acid Hybridization
D004273:DNA, Neoplasm