On the role of sulfhydryl groups in the structure and function of the Azotobacter vinelandii RNA polymerase.
Exposure of sulfhydryl groups as indicated by titration kinetics is decreased under conditions where RNA polymerase exists as a dimer or higher aggregate (low salt), in the presence of Mn2+, or when bound to d(A-T). Incubation of phenylmercurisulfonate with RNA polymerase above pH 9.0 results in loss of d(A-T) binding ability. Poly(U) binding is more sensitive to sulfhydryl modification and is lost as pH's above 8.0. The presence of 4 mM Mn2+ has an obvious effect in stabilizing the polymerase-poly(U) complex when incubated with 10 muM phenylmercurisulfonate + 1 M urea. Incubation of the enzyme with the mercurial and urea results in disaggregation to subprotomeric forms and release of the alpha subunit. Similar treatment in the presence of 4 mM MnSO4 stabilizes the protomeric structure of the enzyme. During chain elongation the enzyme exists as a ternary d(A-T)n-enzyme-r(U-A)n complex in which the bound d(A-T)n is refractory to the destabilizing effect of the mercurial; however, further phosphodiester bond formation is inhibited. The results are defined in terms of a role which reflects the involvement of polymerase sulfhydryl groups in the various conformations necessary for subunit-subunit interaction, tight template binding and catalytic activity.
D001395:Azotobacter / Q000201:enzymology*; D012321:DNA-Directed RNA Polymerases / Q000032:analysis / Q000378:metabolism*; D004355:Drug Stability; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D009994:Osmolar Concentration; D011072:Poly U; D011189:Potassium Chloride / Q000494:pharmacology; D011485:Protein Binding; D013438:Sulfhydryl Compounds / Q000032:analysis; D013698:Templates, Genetic
D013438:Sulfhydryl Compounds; D011072:Poly U; D008345:Manganese; D011189:Potassium Chloride; D012321:DNA-Directed RNA Polymerases; D008274:Magnesium