Polypeptides of simian rotavirus (SA-11) determined by a continuous polyacrylamide gel electrophoresis method.
Simian rotavirus (SA-11) isolated from infected African green monkey kidney cells was separated into two virus fractions in a CsCl density gradient and their proteins analysed on a continuous phosphte buffered polyacrylamide gel electrophoresis system. One peak (buoyant density 1.37 g/ml) contained double capsid virus particles which were radioimmunoassay (RIA)- and haemagglutinin (HA)- positive and yielded eight polypeptides whose mol. wt. ranged from 48,000 to 128,000. The second peak (buoyant density 1.39 g/ml) which contained 70% single capsid particles and was RIA-positive but HA-negative, yielded only five polypeptides. The three polypeptides missing in the second peak are associated presumably with the outer capsid of SA-11 virus particles and one or more of these is assumed to be the HA of SA-11 rotavirus.
D000818:Animals; D002213:Capsid / Q000032:analysis; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D000882:Haplorhini; D007668:Kidney; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis*; D012328:RNA Viruses / Q000032:analysis*; D012401:Rotavirus / Q000032:analysis*; D014764:Viral Proteins / Q000032:analysis*
D010455:Peptides; D014764:Viral Proteins