title: Serological incidence of avian reovirus infection in broiler-breeders and progeny in Nova Scotia.
abstract: The plaque neutralization test and the agar gel precipitation test were used to detect neutralizing and precipitating antibody to avian reovirus strain WVU2937 in sera from 14 commercial broiler breeder flocks and eight progeny flocks. Ten breeder flocks (71%) possessed positive agar gel precipitation reactors (598 sera tested) and 12 (86%) possessed plaque neutralization reactors (114 serum pools tested). All broiler flocks possessed agar gel precipitation reactors, but these were not examined for neutralizing antibody. As no avian reovirus vaccine had been used in Canada prior to this survey these reactions were presumably due to natural infection. Clinical evidence suggested that such infection was asymptomatic in most cases. The development and persistence of antibody to reovirus strain WVU2937 was followed in chicks exposed to the virus by oral inoculation or by contact. Neutralizing antibody was demonstrated in all birds by postinoculation day 17 and this persisted for at least six months. Precipitating antibody was also demonstrated in most chicks by postinoculation day 17, but by six months only 12% of the chicks were still agar gel precipitation positive.
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002645:Chickens; D005779:Immunodiffusion; D009674:Nova Scotia; D011201:Poultry Diseases / Q000276:immunology*; D012088:Reoviridae Infections / Q000276:immunology / Q000662:veterinary*
chemical_list: D000914:Antibodies, Viral