Subcellular distribution of amino acid naphthylamidase isozymes in human leucocytes.
Three isozymes (A, B and C) of amino acid naphthylamidase were found in human leucocytes. Isozyme B and C had close biochemical similarities and differed from isozyme A. Isozyme A was localized in the cytosol. Results obtained by combining electrophoresis with gradient centrifugation suggested that isozyme B originated in granules of polymorphonuclear leucocytes enriched in alkaline phosphatase. Isozyme C was found in granules of low density of polymorphonuclear as well as mononuclear leucocytes.
D000469:Alkaline Phosphatase / Q000097:blood; D000626:Aminopeptidases / Q000097:blood*; D002499:Centrifugation, Density Gradient; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000097:blood*; D007962:Leukocytes / Q000201:enzymology*
D007527:Isoenzymes; D000469:Alkaline Phosphatase; D000626:Aminopeptidases