title: Regulation of fatty acid synthetase activity. The 4'-phosphopantetheine hydrolase of rat liver.
abstract: The 4'-phosphopantetheine hydrolase of rat liver, partially purified by ammonium sulfate precipitation, catalyzes the hydrolysis of the prosthetic group 4'-phosphopantetheine from the holo-fatty acid synthetase. The two products of the action of this enzyme, 4'-phosphopantetheine and apo-fatty acid synthetase, were isolated by DEAE-cellulose chromatography and by chromatography on a Sepharose epsilon-aminocaproyl pantetheine column, respectively. The resultant apo-fatty acid synthetase was quantitated by immunoprecipitation and it was also converted to the holoprotein with a crude preparation of rat liver 4'-phosphopantetheine transferase. Quantitative determination of the hydrolase reaction product, 4'-phosphopantetheine, by amino acid analysis and microbiological assays confirmed the presence of 1 mol of this compound/mol of holo-fatty acid synthetase.
mesh_terms: D000213:Acyl Carrier Protein; D000818:Animals; D001051:Apoenzymes / Q000378:metabolism; D064429:Fatty Acid Synthases / Q000378:metabolism*; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008297:Male; D010204:Pantetheine / Q000031:analogs & derivatives; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D010770:Phosphotransferases / Q000378:metabolism; D051381:Rats; D017855:Transferases (Other Substituted Phosphate Groups)
chemical_list: D000213:Acyl Carrier Protein; D001051:Apoenzymes; D010204:Pantetheine; D064429:Fatty Acid Synthases; D010770:Phosphotransferases; D017855:Transferases (Other Substituted Phosphate Groups); C021364:holo-(acyl-carrier-protein) synthase; D010727:Phosphoric Diester Hydrolases