title: Practical aerobic membrane filtration blood culture technique: development of procedure.
abstract: The advantages of a membrane filter system for blood culturing have been realized for many years. Lysing of the blood prior to filtration is a convenient way to proceed, but previously described lysing procedures result in loss of certain organisms, particularly gram-negative bacilli. Four concentrations of Triton X-100 and sodium carbonate were studied in vitro, and their lysing and antibacterial properties were observed. A solution of 0.08% Na2CO3 and 0.005% Triton X-100 was found to have the least antibacterial effect and gave consistently good lysis and filtration times (under 3 min). An 8.3-ml amount of blood added to 190 ml of this concentration of lysing solution, filtered through three 47-mm membrane filters (0.45-mum pore size), led to recovery of 85% or more of various aerobic and facultative organisms in studies of artificially seeded blood.
mesh_terms: D000332:Aerobiosis; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001419:Bacteria / Q000302:isolation & purification*; D002254:Carbonates / Q000494:pharmacology; D003937:Diagnosis, Differential; D004926:Escherichia coli / Q000187:drug effects / Q000302:isolation & purification; D006461:Hemolysis; D006801:Humans; D008851:Micropore Filters; D011092:Polyethylene Glycols / Q000494:pharmacology; D018805:Sepsis / Q000175:diagnosis*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000302:isolation & purification; D013296:Streptococcus pneumoniae / Q000187:drug effects / Q000302:isolation & purification; D014462:Ultrafiltration / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents; D002254:Carbonates; D011092:Polyethylene Glycols