[Effects of the medium on the rate of electron transfer in a reconstituted system of mitochondrial hydroxylation].
Effects of ionic strength, pH, viscosity, concentrations of components and nature of acceptor on the rate of NADPH oxidation and acceptor reduction were studied in a hydroxylation system containing adrenodoxin reductase, adrenodoxin and cytochrome P450 or cytochrome c. The maximal rate was observed with 0.05--0.10 M phosphate buffer, pH 6.0--6.5 and at the adrenoxin/flavoprotein/cytochrome ratio of 1 : 1 : 1. The electron transfer rate was decreased with an increase in viscosity. Cytochrome P450 is more efficient as a terminal acceptor as compared to cytochrome c or indigodisulphonate.
D000325:Adrenodoxin / Q000378:metabolism*; D000818:Animals; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D003574:Cytochrome c Group / Q000378:metabolism*; D004579:Electron Transport; D005287:Ferredoxin-NADP Reductase / Q000378:metabolism*; D006900:Hydroxylation; D007700:Kinetics; D008928:Mitochondria / Q000378:metabolism*; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D014783:Viscosity
D003574:Cytochrome c Group; D000325:Adrenodoxin; D003577:Cytochrome P-450 Enzyme System; D005287:Ferredoxin-NADP Reductase; D009247:NADH, NADPH Oxidoreductases
Vliianie spedy na skorost' elektronnogo perenosa v rekonstruirovannoĭ sisteme mitokhondrial'nogo gidroksilirovaniia.