[Reproduction of murine encephalomyocarditis virus in enucleated Krebs 2 ascitic carcinoma cells].
Enucleated cells of ascites carcinoma Krebs 2 (cytoplasts) were prepared. The cells were centrifugated for this purpose in a discontinuous Ficoll density gradient containing cytochalasine B. The cytoplasts formed turbid bands in Phycol solution at the density of 1.037--1.053 g/ml. The cytoplasts failed to synthesize RNA, but proteins were synthesized for several hours. Encephalomyocarditis virus replicated in the ascites carcinoma cytoplasts, but its yield was 10 to 100-fold lower as compared to that in the intact cells. Apparently the reduction of the virus yield was not due to the low efficiency of early stages of the virus-cytoplast interaction. Viral infection resulted in inhibition of the synthesis of cellular proteins in the cytoplasts as well as in the intact cells.
D000818:Animals; D002287:Carcinoma, Krebs 2 / Q000378:metabolism / Q000382:microbiology*; D002467:Cell Nucleus / Q000382:microbiology*; D002478:Cells, Cultured; D004686:Encephalomyocarditis virus / Q000502:physiology*; D005260:Female; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis; D051381:Rats; D014779:Virus Replication
D012313:RNA
Reproduktsiia virusa éntsefalomiokardita mysheĭ v bez"iadernykh kletkakh astsitnoĭ kartsinomy Krebs 2.