title: Polyoma viruses with mutations at endonuclease HindII site 1: alterations at the COOH terminus of VP1.
abstract: Four mutants of polyoma virus lacking endonuclease HindII site 1 were isolated and characterized with respect to the VP1 coding sequence. Three of these mutants had deletions that removed 0.2 to 0.3% of the genome. All three deletion mutants encoded VP1 proteins that were smaller than wild type and that lacked one or more tryptic peptides normally found in the wild-type VP1 protein. Our results suggest the HindII site 1 is at, or very near, the carboxy terminal end of the coding sequence for VP1. A model for the peptide organization in that region is presented.
mesh_terms: D002213:Capsid / Q000032:analysis / Q000235:genetics*; D004262:DNA Restriction Enzymes / Q000494:pharmacology; D004279:DNA, Viral / Q000235:genetics*; D004720:Endonucleases / Q000494:pharmacology; D005796:Genes; D005814:Genes, Viral; D008954:Models, Biological; D009154:Mutation; D011120:Polyomavirus / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins; D004720:Endonucleases; D004262:DNA Restriction Enzymes