Inhibition of human lymphocyte mitogen and antigen response by a 15,000-dalton protein from feline leukemia virus.
Peripheral blood lymphocyte response of normal human subjects to mitogens and antigens was suppressed by a 15,000-dalton protein (p15) from a C-type feline leukemia virus. Four of six subjects were suppressed 70 to 96% when responding to concanavalin A or phytohemagglutinin in the presence of 5.0 microgram of p15. The three subjects who responded to streptokinase-streptodornase and Candida were suppressed 68 to 91% when cultured with 5.0 microgram of p15. The subviral protein did not appear to be cytotoxic at doses reported. Lymphocyte membrane studies with fluorescein isothiocyanate-concanavalin A revealed a reduction in concanavalin A-induced cap formation of 51 to 91% in the presence of the same dose of p15. These results demonstrate that in vitro immunological dysfunction in human lymphocytes can be induced by a C-type virion protein.
D000941:Antigens / Q000008:administration & dosage; D002470:Cell Survival; D006801:Humans; D007152:Immunologic Capping; D007165:Immunosuppression Therapy; D066298:In Vitro Techniques; D005256:Leukemia Virus, Feline / Q000276:immunology*; D008213:Lymphocyte Activation; D008934:Mitogens / Q000494:pharmacology; D008970:Molecular Weight; D009363:Neoplasm Proteins / Q000276:immunology*; D014764:Viral Proteins / Q000276:immunology*
D000941:Antigens; D008934:Mitogens; D009363:Neoplasm Proteins; D014764:Viral Proteins