Granules and Golgi vesicles with differential reactivity to ACTH antiserum in the corticotroph of the rat anterior pituitary.
Slices of unembedded rat anterior pituitaries, fixed with a periodate-lysine-paraformaldehyde (PLP) fixative, were incubated with guinea pig antiserum to ACTH and stained with a peroxidase-conjugated IgG fraction of anti-guinea pig gamma-globulin serum from rabbits. The fine structure of the stained cells was identical to that of the ACTH-secreting cell, as described by Siperstein and coworkers. Immunoreactive granules were mainly located at the periphery of the cell. Numerous granules of the inner cytoplasm and also the Golgi complex were nonreactive to the antiserum. The differential labeling for granules and Golgi apparatus peptide.
D000135:Acid Phosphatase / Q000032:analysis; D000324:Adrenocorticotropic Hormone / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D000818:Animals; D003594:Cytoplasmic Granules / Q000032:analysis*; D005260:Female; D006056:Golgi Apparatus / Q000032:analysis*; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D010903:Pituitary Gland, Anterior / Q000032:analysis / Q000378:metabolism / Q000648:ultrastructure*; D051381:Rats
D007106:Immune Sera; D000324:Adrenocorticotropic Hormone; D000135:Acid Phosphatase