The kinetics of pancreatic ribonuclease reaction with alkaline and acidic forms of poly A.
The RNase hydrolysis of random-coil (alkaline form) poly A follows biphasic kinetics at low salt concentrations. However, its resistance to RNase increases with the ionic strength. Helical (acidic form) poly A is alos susceptible to RNase but its hydrolysis follows first-order kinetics, and its resistance increases as the pH is lowered. These conformation-dependent kinetics of poly A hydrolysis are similar to those obtained in the hydrolysis of cellular RNA and reovirus double-stranded RNA.
D000818:Animals; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D009994:Osmolar Concentration; D010179:Pancreas / Q000201:enzymology*; D011061:Poly A; D012260:Ribonucleases / Q000378:metabolism*
D011061:Poly A; D012260:Ribonucleases; D008274:Magnesium