Met-enkephalin: rapid separation from brain extracts using high-pressure liquid chromatography, and quantitation by binding assay.
Met-enkephalin can be rapidly separated with high recovery from leu-enkephalin, beta-endorphin, and enkephalin metabolites by high-pressure liquid chromatography on a Dupont SCX column. The technique permits separation of endogenous enkephalins from brain extracts for measurement by opiate-receptor binding assay, immunoassay, or for study of the incorporation of radiolabeled amino acids into enkephalin. Using this technique, met-enkephalin was separated from striatal extracts of rats killed by focused microwave irradiation and quantitated by the opiate-receptor binding assay. The concentration of met-enkephalin in the column eluate was 1.3 micron/g tissue. This value is comparable to that obtained by radioimmunoassay without purification of the extracts.
D000818:Animals; D001681:Biological Assay; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004723:Endorphins / Q000302:isolation & purification*; D004745:Enkephalins / Q000302:isolation & purification* / Q000378:metabolism; D008297:Male; D011863:Radioimmunoassay / Q000379:methods; D051381:Rats; D011957:Receptors, Opioid / Q000378:metabolism
D004723:Endorphins; D004745:Enkephalins; D011957:Receptors, Opioid