title: Methodological studies on the uptake of zinc by 3T3 cells.
abstract: In the present work, experiments were conducted on the uptake of zinc by 3T3 cells. (1) The percent Zn uptake gradually decreased with addition of increasing amount of zinc (0.05-8.4 mug). (2) With the increase of the incubation period from 2 to 16 hr, Zn uptake by nearly confluent cells increases gradually; however, confluent cells which are newly replated show a distinct cyclic increase in the Zn uptake after 2, 6, and 10 hr. (3) The amino acids of DMM and serum decrease Zn uptake. (4) Histidine at a molar excess of 1:50, 1:500, and 1:5000 reduces Zn uptake in comparison to a treatment with 65Zn-Zn-L-hist2 at a molar ratio of 1:5. (5) When zinc is added in the form of different Zn compounds, at a molar ratio of 1:2 or 1:1 (Zn:ligand), EDTA decreases the Zn uptake markedly. A small influence was shown also by albumin and histidine; however, other amino and organic acids at a molar ratio of 1:2 did not alter Zn uptake significantly.
mesh_terms: D000818:Animals; D002460:Cell Line; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D005029:Ethylenediamines / Q000494:pharmacology; D005650:Fumarates / Q000494:pharmacology; D005998:Glycine / Q000494:pharmacology; D006639:Histidine / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D051379:Mice; D013912:Threonine / Q000494:pharmacology; D013997:Time Factors; D014633:Valine / Q000494:pharmacology; D015032:Zinc / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D003470:Culture Media; D005029:Ethylenediamines; D005650:Fumarates; D013912:Threonine; D006639:Histidine; D014633:Valine; D015032:Zinc; D005998:Glycine