Production of human lymphocyte mediators after activation with periodate or neuraminidase and galactose oxidase.
Treatment of human mononuclear cells with sodium metaperiodate (NaIO4) or neuraminidase and galactose oxidase (NGO) results in lymphocyte activation and subsequent generation of supernatants rich in migration inhibitory factor (MIF) and leukocyte inhibitory factor (LIF). Preliminary characterization of these mediators by Sephadex G-100 gel filtration suggests that they are similar to antigen- and concanavalin A-induced MIF and LIF, eluting in the 25000 m.w. and 68000 m.w. regions, respectively. The possibility of galactose oxidase carryover into the supernatants has been studied and conditions are described that minimize this eventuality. A method is presented for producing control and lymphokine-rich supernatants both of which have been exposed to identical concentrations of NGO although in the control activation is blocked by the addition of 0.1 M galactose to the incubation. These findings establish NaIO4 and NGO as useful mitogens for generating human lymphokine-rich supernatants that can be used without further purification.
D002850:Chromatography, Gel; D005692:Galactose Oxidase / Q000494:pharmacology*; D006801:Humans; D007961:Leukocyte Migration-Inhibitory Factors / Q000096:biosynthesis; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000201:enzymology; D008222:Lymphokines / Q000096:biosynthesis*; D008263:Macrophage Migration-Inhibitory Factors / Q000096:biosynthesis; D009439:Neuraminidase / Q000494:pharmacology*; D010504:Periodic Acid / Q000494:pharmacology*; D013997:Time Factors
D007961:Leukocyte Migration-Inhibitory Factors; D008222:Lymphokines; D008263:Macrophage Migration-Inhibitory Factors; D010504:Periodic Acid; D005692:Galactose Oxidase; D009439:Neuraminidase