Mechanism of action of collagenase on the blood-brain barrier permeability. Increase of endothelial cell pinocytotic activity as shown with horse-radish peroxidase as a tracer.
The ultrastructural mechanism of the protease induced blood-brain barrier permeability-increase was studied with horse-radish peroxidase as a tracer. After intravenous injection of collagenase or pronase, a significantly increased number of pinocytotic vesicles was found in brain capillary endothelial cells. alpha-Chymotrypsine did not exert such an action.
D000818:Animals; D001812:Blood-Brain Barrier / Q000187:drug effects*; D001921:Brain / Q000098:blood supply; D002196:Capillaries / Q000502:physiology / Q000648:ultrastructure; D002918:Chymotrypsin / Q000494:pharmacology; D004727:Endothelium / Q000166:cytology; D006735:Horseradish Peroxidase; D008297:Male; D003012:Microbial Collagenase / Q000494:pharmacology*; D010539:Permeability; D010873:Pinocytosis / Q000187:drug effects; D011402:Pronase / Q000494:pharmacology; D051381:Rats
D006735:Horseradish Peroxidase; D002918:Chymotrypsin; D011402:Pronase; D003012:Microbial Collagenase