EAC4 and EAC14 production without purified Ci.
EAC4 and EAC14 of high activity were prepared by treating sensitized red cells with human or guinea pig serum in the presence of TTHA, a chelating agent whid at low pH to maximize C1 and C4 activity and to minimize C3 contamination. Cells prepared with guinea pig complement were contaminated with C2, which could be decayed away at 37 degrees C. Cells sensitized with IgG antibody were more reactive than those sensitized with whole serum or with IgM, and preparations made with TTHA-complement were more reactive than those prepared with purified C1 plus EDTA-complement. EAC14 stored at 0 degrees C for 3 weeks lost very little activity.
D000818:Animals; D000906:Antibodies; D000936:Antigen-Antibody Complex; D001666:Binding Sites, Antibody; D002021:Buffers; D002614:Chelating Agents / Q000494:pharmacology; D003172:Complement C1 / Q000378:metabolism*; D003176:Complement C3; D003181:Complement C4 / Q000378:metabolism*; D003165:Complement System Proteins / Q000378:metabolism*; D004492:Edetic Acid; D004912:Erythrocytes / Q000276:immunology; D006168:Guinea Pigs; D006461:Hemolysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007104:Immune Adherence Reaction; D012756:Sheep
D000906:Antibodies; D000936:Antigen-Antibody Complex; D002021:Buffers; D002614:Chelating Agents; D003172:Complement C1; D003176:Complement C3; D003181:Complement C4; D003165:Complement System Proteins; D004492:Edetic Acid