title: Effect of cyclic nucleotides on weight of gastrocnemius and creatine kinase activity after denervation of muscle in young rats.
abstract: Denervation of the gastrocnemius muscle at various stages of development reduces muscle weight and creatine kinase activity. An inverse relationship between the muscle-specific enzyme, creatine kinase, and the lysosomal enzyme, acid phosphatase, was shown. An increased percentage of the BB isoenzyme of creatine kinase is observed after long-term denervation. Apparently the muscle tissue has the ability to regenerate and presumptive myoblasts are formed from satellite cells. When the denervated muscle is treated with dibutyryl-cyclic-GMP administration new muscle tissue has been formed. Similar effects could not be demonstrated with either cyclic AMP or succinylcholine. The higher percentage of the BB isoenzyme after dibutyryl-cyclic-GMP administration supports the theory that presumptive myoblasts are derived from satellite cells. Succinylcholine also causes an increase of the B-type of creatine kinase. It can be concluded that cyclic GMP, generated via the nerve, has an important role in maintaining muscle weight.
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000367:Age Factors; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D003402:Creatine Kinase / Q000378:metabolism*; D000242:Cyclic AMP / Q000494:pharmacology*; D006152:Cyclic GMP / Q000494:pharmacology*; D003995:Dibutyryl Cyclic GMP / Q000494:pharmacology; D007527:Isoenzymes; D009121:Muscle Denervation; D009132:Muscles / Q000187:drug effects* / Q000201:enzymology / Q000294:innervation; D009929:Organ Size / Q000187:drug effects; D051381:Rats
chemical_list: D007527:Isoenzymes; D003995:Dibutyryl Cyclic GMP; D003994:Bucladesine; D000242:Cyclic AMP; D003402:Creatine Kinase; D000135:Acid Phosphatase; D006152:Cyclic GMP