A purine nucleoside hydrolase from Trypanosoma gambiense, purification and properties.
A purine nucleoside hydrolase from Trypanosoma gambiense was purified 160-fold. Preferred substrates of the reaction were adenosine, inosine and guanosine with a maximum of activity at pH 5.4. Competitive inhibitors of the adenosine hydrolysis were dimethylallyl adenosine, 6-methylmercaptopurine riboside, tubercidin, formycin B, 6-mercaptopurine riboside and deoxyadenosine. A metabolic scheme of adenosine nomophosphate salvage synthesis is discussed.
D000228:Adenine Phosphoribosyltransferase / Q000302:isolation & purification; D000241:Adenosine / Q000378:metabolism; D000248:Adenosine Kinase / Q000302:isolation & purification; D000249:Adenosine Monophosphate / Q000096:biosynthesis; D000818:Animals; D002474:Cell-Free System; D002845:Chromatography; D002848:Chromatography, DEAE-Cellulose; D006151:Guanosine; D006863:Hydrogen-Ion Concentration; D007041:Hypoxanthine Phosphoribosyltransferase / Q000302:isolation & purification; D007288:Inosine; D007525:Isoelectric Focusing; D008025:Ligases / Q000302:isolation & purification; D009699:N-Glycosyl Hydrolases / Q000037:antagonists & inhibitors / Q000302:isolation & purification*; D010085:Oxidative Phosphorylation; D011684:Purine Nucleosides; D011685:Purine Nucleotides / Q000096:biosynthesis; D014347:Trypanosoma brucei gambiense / Q000201:enzymology* / Q000378:metabolism
D011684:Purine Nucleosides; D011685:Purine Nucleotides; D006151:Guanosine; D000249:Adenosine Monophosphate; D007288:Inosine; D000228:Adenine Phosphoribosyltransferase; D007041:Hypoxanthine Phosphoribosyltransferase; D000248:Adenosine Kinase; D009699:N-Glycosyl Hydrolases; D008025:Ligases; D000241:Adenosine