The differentiation of N-oxidation and N-dealkylation of N-ethyl-N-methylaniline by rabbit liver microsomes as distinct metabolic routes.
1. N-Oxidation and N-dealkylation were shown to be separate routes of metabolism for N-ethyl-N-methylaniline. 2. N-Dealkylation, but not N-oxidation, is enhanced when NADH is present in addition to the NADPH-regenerating cofactors. 3. Some inhibitors, e.g. p-chloromercuribenzoate, inhibit N-dealkylation to a very much greater extent than they do N-oxidation. 4. N-Dealkylation is affected more by 'ageing' of the microsomal preparation or by the presence of bile salts, than is N-oxidation. 5. N-Oxidation followed by N-O-alpha-carbon migration of oxygen is only implicated to a very minor extent in the N-dealkylation process.
D015082:2,2'-Dipyridyl / Q000494:pharmacology; D000096:Acetone / Q000494:pharmacology; D000814:Aniline Compounds / Q000378:metabolism*; D000818:Animals; D002729:Chloromercuribenzoates / Q000494:pharmacology; D003543:Cysteamine / Q000494:pharmacology; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D003640:Dealkylation; D004229:Dithiothreitol / Q000494:pharmacology; D005033:Ethylmaleimide / Q000494:pharmacology; D007700:Kinetics; D008099:Liver / Q000378:metabolism; D008297:Male; D008797:Metyrapone / Q000494:pharmacology; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D009249:NADP / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism*; D011335:Proadifen / Q000494:pharmacology; D051381:Rats; D013347:Subcellular Fractions / Q000378:metabolism
D000814:Aniline Compounds; D002729:Chloromercuribenzoates; D000096:Acetone; D009249:NADP; D015082:2,2'-Dipyridyl; D003543:Cysteamine; D003577:Cytochrome P-450 Enzyme System; D011335:Proadifen; D010088:Oxidoreductases; D005033:Ethylmaleimide; D004229:Dithiothreitol; D008797:Metyrapone