Simian virus 40-transformed cells express new species of proteins precipitable by anti-simian virus 40 tumor serum.
In addition to the virus-coded large-T and small-t antigens, two new classes of proteins were immunoprecipitated by anti-simian virus 40 (SV40) tumor serum from extracts of various SV40-transformed cell lines. These were as follows: (i) proteins (termed "super-T proteins") with an Mr higher than that of large-T antigen (86,000), which were found in many SV40-transformed cell lines derived from mouse and rat cells (super-T proteins and large-T antigen appeared to have closely related structures as judged by the Chromobead elution patterns of their methionine-labeled tryptic peptides); (ii) proteins (termed "55K proteins") with an Mr ranging from 50,000 to 60,000, which were present in all SV40-transformed cell lines examined so far, including those obtained by chromosome-mediated gene transfer. The 55K proteins were not structurally related to large-T antigens, as judged by the Chromobead elution patterns of their methionine-labeled tryptic peptides. Our data are compatible with the assumption that the 55K proteins are largely or totally cell coded.
D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis*; D000956:Antigens, Viral / Q000032:analysis*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D006224:Cricetinae; D000882:Haplorhini; D006801:Humans; D007106:Immune Sera; D007668:Kidney; D008168:Lung / Q000196:embryology; D051379:Mice; D009374:Neoplasms, Experimental / Q000276:immunology; D051381:Rats; D013539:Simian virus 40 / Q000032:analysis / Q000276:immunology*; D012867:Skin; D014764:Viral Proteins / Q000032:analysis / Q000276:immunology*
D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D007106:Immune Sera; D014764:Viral Proteins