[Antigen enzyme coupling: a few results (author's transl)].
Various technics of coupling (carbodiimide, glutaraldehyde, periodate) have been used for the grafting of human casein on glucose oxidase. The various types of product obtained were identified by gel filtration and used for drawing up titration curves. The authors compared radioimmunoassay and enzymoimmunologic estimation showing the importance of the molecular size of the conjugates obtained.
D000941:Antigens; D002234:Carbodiimides; D002364:Caseins / Q000276:immunology*; D055598:Chemical Phenomena; D002621:Chemistry; D005949:Glucose Oxidase / Q000276:immunology*; D005976:Glutaral; D007124:Immunoenzyme Techniques; D008895:Milk, Human; D010504:Periodic Acid; D011863:Radioimmunoassay
D000941:Antigens; D002234:Carbodiimides; D002364:Caseins; D010504:Periodic Acid; D005949:Glucose Oxidase; D005976:Glutaral
Couplage antigène-enzyme: quelques résultats.