The effect of transmural field stimulation on the serotonin content in rat duodenal enterochromaffin cells--in vitro.
The effect of transmural field stimulation (TFS)--in vitro--on the sertonin (5-HT) content in enterochromafffin cells (EC) in rat duodenum was studied with a cytofluorimetric method. TFS caused a significant 25% decrease of 5-HT in EC. The presence of tetrodotoxin or d,1-propranolol in the stimulation bath antagonized the effect of TFS. In biopsies from rats pretreated with 6-OH-dopamine TFS had no effect of the 5-HT content in EC. The results of the present study strongly suggest, that the TFS induced decrease in 5-HT content is due to a direct neural, probably beta-adrenoceptor mediated, influence on the EC.
D000320:Adrenergic Fibers / Q000502:physiology; D000818:Animals; D002838:Chromaffin System / Q000502:physiology*; D004386:Duodenum / Q000166:cytology / Q000294:innervation; D004558:Electric Stimulation; D004759:Enterochromaffin Cells / Q000032:analysis / Q000502:physiology*; D005470:Fluorometry; D006892:Hydroxydopamines / Q000494:pharmacology; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000166:cytology; D008297:Male; D008954:Models, Biological; D011433:Propranolol / Q000494:pharmacology; D051381:Rats; D011943:Receptors, Adrenergic, beta / Q000187:drug effects; D012701:Serotonin / Q000032:analysis / Q000378:metabolism*; D013779:Tetrodotoxin / Q000494:pharmacology
D006892:Hydroxydopamines; D011943:Receptors, Adrenergic, beta; D012701:Serotonin; D013779:Tetrodotoxin; D011433:Propranolol