Virus-specific DNA sequences present in cells which carry the herpes simplex virus thymidine kinase gene.
Two independently derived cell lines which carry the herpes simplex type 2 thymidine kinase gene have been examined for the presence of HSV-2-specific DNA sequences. Both cell lines contained 1 to 3 copies per cell of a sequence lying within map co-ordinates 0.2 to 0.4 of the HSV-2 genome. Revertant cells, which contained no detectable thymidine kinase, did not contain this DNA sequence. The failure of EcoR1-restricted HSV-2 DNA to act as a donor of the thymidine kinase gene in transformation experiments suggests that the gene lies close to the EcoR1 restriction site within this sequence at a map position of approx. 0.3. The HSV-2 kinase gene is therefore approximately co-linear with the HSV-1 gene.
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D004273:DNA, Neoplasm / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis*; D005814:Genes, Viral; D051379:Mice; D009154:Mutation; D009693:Nucleic Acid Hybridization; D018139:Simplexvirus / Q000032:analysis* / Q000235:genetics; D013937:Thymidine Kinase / Q000235:genetics
D004273:DNA, Neoplasm; D004279:DNA, Viral; D013937:Thymidine Kinase