Interaction of polyene antibiotics with sterols in phosphatidylcholine bilayer membranes as studied by spin probes.
Interaction of filipin and amphotericin B with sterols in phosphatidylcholine membranes has been studied using various spin probes; epiandrosterone, cholestanone, phosphatidylcholine with 12-nitroxide or 5-nitroxide stearate attached to 2 position and also with tempocholine at the head group. Filipin caused increase in the fluidity of cholesterol-containing phosphatidylcholine membranes near the center, while it rather decreased the fluidity near the polar surface. On the other hand, amphotericin B did not apparently affect the fluidity. In the electron spin resonance spectrum of steriod spin probes in the antibiotic-containing membranes, both bound and free signals were observed and the association constant was calculated from the siganal intensity. In the binding of steriods with filipin, both 3 and 17 positions were involved, while the 17 positions was less involved in the binding with amphotericin B. Phase change in the host membrane markedly affected the interaction of filipin with epiandrosterone probe. The bound fraction jumped from 0.4 to 0.8 on going to the crystalline state and increased further with decrease in temperature. The overall splitting of the bound signal also increased on lowering the temperature below phase transition. This change was attributed to aggregate formation of filipin-steriod complexes in the crystalline state. On the other hand, effect of phase transition was much smaller on the interaction of amphotericin B with the steriod probe.
D000666:Amphotericin B; D004578:Electron Spin Resonance Spectroscopy; D005372:Filipin; D007700:Kinetics; D008567:Membranes, Artificial; D008968:Molecular Conformation; D010713:Phosphatidylcholines; D011090:Polyenes; D013113:Spin Labels; D013261:Sterols
D008567:Membranes, Artificial; D010713:Phosphatidylcholines; D011090:Polyenes; D013113:Spin Labels; D013261:Sterols; D000666:Amphotericin B; D005372:Filipin