Differences in flow cytometry and 3H-thymidine analyses of perturbed human lymphocytes.
A calf thymocyte crude aqueous extract was tested for DNA synthesis inhibitory activity using phytohemagglutinin-stimulated human peripheral blood lymphocytes. Inhibition of DNA synthesis was assayed using tritiated thymidine and flow cytometry. Although the calf thymocyte crude extract inhibited tritiated thymidine incorporation by over 50%, only very slight changes in the flow cytometric analysis were observed. When dibutyryl-cyclic adenosine monophosphate was used as an inhibitor, a correlation in terms of the inhibition of tritiated thymidine to the inhibition by flow cytometry was observed.
D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D002417:Cattle; D003584:Cytological Techniques; D004247:DNA / Q000096:biosynthesis*; D006801:Humans; D007376:Interleukin-2 / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D010783:Photometry; D010835:Phytohemagglutinins / Q000494:pharmacology; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D007376:Interleukin-2; D010835:Phytohemagglutinins; D014316:Tritium; D003994:Bucladesine; D004247:DNA; D013936:Thymidine