Inhibition of trypsin and chymotrypsin by thiols. Biphasic kinetics of reactivation and inhibition induced by sodium periodate addition.
Biphasic kinetic data were obtained when trypsin (EC 3.4.21.4) which had previously been complexed with a thiol-containing inhibitor (present in Ehrlich ascites tumour cells) was incubated with incremental additions of periodate. At low concentrations of periodate the trypsin was re-activated whilst at higher concentrations of periodate the trypsin was irreversibly inhibited. This biphasic reactivation followed by inhibition was also demonstrated when trypsin was first inhibited by dithiothreitol and followed by incremental addition of periodate. Similar results were obtained with chymotrypsin (EC 3.4.21.1). Incremental additions of either dithiothreitol or periodate caused inhibition of both these enzymes. The biphasic kinetic data can be explained in terms of reduction and oxidation of a significant disulphide bond in both trypsin and chymotrypsin which can be cleaved by thiols in a disulphide exchange reaction [1]. This bond is thought to maintain the active centres of each of these enzymes in a conformation sterically favourable for enzymic cleavage of specific peptide bonds in the protein substrates (polymeric collagen fibrils and casein) employed in this study.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000201:enzymology; D002918:Chymotrypsin / Q000037:antagonists & inhibitors*; D004229:Dithiothreitol / Q000494:pharmacology*; D004793:Enzyme Reactivators; D066298:In Vitro Techniques; D007700:Kinetics; D051379:Mice; D010504:Periodic Acid / Q000494:pharmacology*; D014361:Trypsin Inhibitors
D004793:Enzyme Reactivators; D014361:Trypsin Inhibitors; D010504:Periodic Acid; D002918:Chymotrypsin; D004229:Dithiothreitol