Variations in the pathways of malate oxidation and phosphorylation in different species of Mycobacteria.
Mycobacterium tuberculosis H37Rv, the slow-growing human pathogenic strain of tubercle bacilli and Mycobacterium smegmatis and Mycobacterium phlei, the fast-growing saprophytes, have shown variations regarding the type of dehydrogenase that initiates malate oxidation in the respiratory chain. M. tuberculosis H37Rv is characterized by having a malate oxidase system (designated MALNAD pathway) in which malate oxidation is mediated by the NAD+-dependent malate dehydrogenase (EC 1.1.1.37) but not by FAD-dependent malate-vitamin K reductase. M. smegmatis possesses a different malate oxidase system (designated MALFAD pathway) in which malate oxidation is exclusively carried out by the FAD-dependent malate-vitamin K reductase because NAD+-dependent malate dehydrogenase is absent in this organism. M. phlei has a mixed system of malate oxidase (designated MALNAD+FAD pathways) in which both the NAD+-and FAD-dependent dehydrogenases take part. In all the three systems, the rest of the electron transport chain is common.
D000429:Alcohol Oxidoreductases / Q000378:metabolism; D005182:Flavin-Adenine Dinucleotide; D014644:Genetic Variation; D008291:Malate Dehydrogenase / Q000378:metabolism; D008293:Malates / Q000378:metabolism*; D009161:Mycobacterium / Q000378:metabolism*; D009169:Mycobacterium tuberculosis / Q000378:metabolism; D009243:NAD; D010085:Oxidative Phosphorylation; D010101:Oxygen Consumption; D013045:Species Specificity; D013053:Spectrophotometry; D013385:Succinate Dehydrogenase / Q000378:metabolism; D014812:Vitamin K
D008293:Malates; D009243:NAD; D014812:Vitamin K; D005182:Flavin-Adenine Dinucleotide; D000429:Alcohol Oxidoreductases; D008291:Malate Dehydrogenase; D013385:Succinate Dehydrogenase