title: Structure of the dimyristoylphosphatidylcholine vesicle and the complex formed by its interaction with apolipoprotein C-III: X-ray small-angle scattering studies.
abstract: Single bilayer vesicles of dimyristoylphosphatidylcholine have been investigated by small-angle X-ray scattering at 28 degrees C. The results indicate that these vesicles are hollow spherical shell structures with an outer radius of approximately 12 nm and a molecular weight of (3.2 +/- 0.5) X 10(6). The shell was found to be 4.4 +/- 0.2 nm thick with a cross-sectional electron-density profile characteristic for a single phospholipid bilayer. Upon interaction of these vesicles with apolipoprotein C-III from human very low density lipoproteins at a protein/lipid ratio greater than 0.08 (g/g), a complex containing 0.25 g of protein/g of lipid, with molecular weight of (3.9 +/- 0.4) X 10(5), is formed. The shape analysis indicates a highly asymmetric particle with an internal partition of low and high electron density resembling that produced by a bilayer structure. Model calculations and curve-fitting procedures show good agreement between the experimental scattering curve and that computed for an oblate ellipsoidal structure with dimensions of 17 X 17 X 5 nm and a 1 nm thick shell of high electron density surrounding the core of low electron density.
mesh_terms: D001053:Apolipoproteins; D006801:Humans; D008079:Lipoproteins, VLDL; D008433:Mathematics; D008567:Membranes, Artificial; D008854:Microscopy, Electron; D008968:Molecular Conformation; D010713:Phosphatidylcholines; D011485:Protein Binding; D011487:Protein Conformation; D012542:Scattering, Radiation; D014965:X-Rays
chemical_list: D001053:Apolipoproteins; D008079:Lipoproteins, VLDL; D008567:Membranes, Artificial; D010713:Phosphatidylcholines