title: [Separation and characteristics of the acid proteinases from a surface culture of Aspergillus terricola].
abstract: The enzymic preparation isolated by ethanol precipitation from the aqueous extract of the surface culture of Aspergillus terricola was purified on biogel P-10, fractionated on SE-Sephadex. As a result, 10 fractions of acid proteinases that differed in pH optima and activity were obtained. After purification specific activity of some fractions per 1 mg protein increased 24-32-fold. The temperature optimum of the enzymes was at 50 degrees C and pH optimum at 2.5--2.8.
mesh_terms: D001230:Aspergillus / Q000201:enzymology*; D002852:Chromatography, Ion Exchange; D003470:Culture Media; D004355:Drug Stability; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D010447:Peptide Hydrolases / Q000032:analysis / Q000302:isolation & purification*; D013696:Temperature
chemical_list: D003470:Culture Media; D010447:Peptide Hydrolases
vernacular_title: Razdelenie i kharakteristika kislykh proteinaz iz poverkhnostnoĭ kul'tury Aspergillus terricola