Isoelectrofocusing in acetate membrane: the method and application.
Acetate membrane as a new medium for isoelectrofocusing was used in this study. The acetate membrane was specially produced for isoelectrofocusing, and contains a surface active agent. The ampholine solution system and isoelectric condition for this purpose are described. Polymorphic traits as alpha1-antitrypsin in human serum and 6-phosphogluconate dehydrogenase (6-PGD) in human erythrocyte could be identified by this method. The isoelectric patterns and the isoelectric points of these proteins were demonstrated on the acetate membrane.
D000085:Acetates; D004912:Erythrocytes / Q000201:enzymology; D014644:Genetic Variation; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing / Q000295:instrumentation / Q000379:methods*; D010641:Phenotype; D010734:Phosphogluconate Dehydrogenase / Q000097:blood*; D000515:alpha 1-Antitrypsin / Q000032:analysis*
D000085:Acetates; D000515:alpha 1-Antitrypsin; D010734:Phosphogluconate Dehydrogenase