Effect of enzymes on rotavirus infectivity.
The infectivity of a bovine rotavirus was enhanced 140-, 8-, and 3-fold, respectively, by trypsin, protease, and lactase. Ficin, carboxypeptidases A and B, lysozyme, and beta-galactosidase had little effect on the infectivity. Chymotrypsin caused a threefold decrease in the infectivity. Trypsin acts directly on the rotavirus and not on the host cell.
D000818:Animals; D002417:Cattle; D002460:Cell Line; D002918:Chymotrypsin / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D006026:Glycoside Hydrolases / Q000494:pharmacology*; D007668:Kidney; D010447:Peptide Hydrolases / Q000494:pharmacology*; D012328:RNA Viruses / Q000187:drug effects*; D012401:Rotavirus / Q000187:drug effects* / Q000254:growth & development / Q000472:pathogenicity; D014357:Trypsin / Q000494:pharmacology; D014774:Virulence; D014779:Virus Replication / Q000187:drug effects*; D001616:beta-Galactosidase / Q000494:pharmacology
D004492:Edetic Acid; D006026:Glycoside Hydrolases; D001616:beta-Galactosidase; D010447:Peptide Hydrolases; D002918:Chymotrypsin; D014357:Trypsin