[Adenylate cyclase activation and its effects on intracellular cAMP in infected KB cells during trypsin-induced infectious Sendaï virus production (author's transl)].
KB cells infected by Sendaï virus can produce infectious virus if they are trypsinated twice over 24 h. Adenylate cyclase activity in infected KB cells is higher and more strongly activated by trypsin than that of control cells, but intracellular concentration of cAMP is the same, except during a short time after trypsinations, especially after the second trypsination which causes infectious virus production. During this short time, intracellular cAMP is slightly higher in infected cells. This miseffect of adenylate cyclase activation on intracellular cAMP concentrations might be related to an increased cell permeability caused by trypsin.
D000262:Adenylyl Cyclases / Q000378:metabolism*; D002460:Cell Line; D000242:Cyclic AMP / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D010222:Parainfluenza Virus 1, Human / Q000502:physiology; D018184:Paramyxoviridae Infections / Q000378:metabolism*; D014357:Trypsin / Q000494:pharmacology; D014779:Virus Replication
D000242:Cyclic AMP; D014357:Trypsin; D000262:Adenylyl Cyclases
Activation de l'adénylate cyclase et ses répercussions sur le taux intracellulaire d'AMP cyclique dans les cellules KB infectées produisant du virus Sendaï infectieux sous l'influence de la trypsine.