title: Genomic RNA of the murine coronavirus JHM.
abstract: Genomic RNA extracted from the purified murine coronavirus JHM sediments between 52S and 54S in aqueous sucrose gradients. The RNA is single-stranded and has an apparent mol. wt. of 5.4 to 6.5 X 10(6), as determined by electrophoresis in polyacrylamide agarose gels of different concentrations. The presence of polyadenylate sequences in the RNA is demonstrated by binding to oligo-)dT) cellulose and digestion with ribonucleases A and T1. The purified RNA does not dissociate into subunits at high temperatures or in high concentrations of DMSO and is infectious.
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D005814:Genes, Viral; D006358:Hot Temperature; D051379:Mice; D008970:Molecular Weight; D006517:Murine hepatitis virus / Q000032:analysis*; D011061:Poly A / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis* / Q000494:pharmacology; D012260:Ribonucleases / Q000378:metabolism
chemical_list: D012367:RNA, Viral; D011061:Poly A; D012260:Ribonucleases