title: The reaction of Rhodospirillum rubrum cytochrome c2 with iron hexacyanides.
abstract: The reaction of Rhodospirillum rubrum cytochrome c2 with the nonphysiological reactants, ferrocyanide and ferricyanide has been investigated as a function of ionic strength, temperature and pH, using both stopped-flow and temperature-jump kinetic methods. The results are consistent with a complex reaction mechanism involving the formation of two intermediate complexes. The site of electron transfer appears to be at the front of the cytochrome c2 molecule near the hem e crevice with interacton of both ferri and ferrocyanide with a positively charged region of the molecule. Comparison of the proposed electron transfer mechanism of cytochrome c2 with ferro-ferricyanide is made with the mechanism proposed based upon structural considerations.
mesh_terms: D001665:Binding Sites; D003580:Cytochromes / Q000378:metabolism; D005292:Ferricyanides; D005295:Ferrocyanides; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008433:Mathematics; D009994:Osmolar Concentration; D010084:Oxidation-Reduction; D011485:Protein Binding; D012247:Rhodospirillum rubrum / Q000201:enzymology*; D012965:Sodium Chloride; D013696:Temperature; D013997:Time Factors
chemical_list: D003580:Cytochromes; D005292:Ferricyanides; D005295:Ferrocyanides; D012965:Sodium Chloride