ACTH radioimmunocytochemistry (RICH) on rat anterior pituitary cells.
Radioimmunocytochemistry (RICH) was applied to detect corticotrophs in adult rat pituitaries and 8-day-old anterior pituitary monolayers by incubating sections and cultures with 125I-ACTH-anti ACTH immune complexes. After incubations autoradiography was made. In comparison, "conventional" immunostaining was carried out on adjacent sections and parallel cultures. It has been established that RICH is suitable for detection of corticotrophs.
D000324:Adrenocorticotropic Hormone / Q000032:analysis*; D000818:Animals; D001345:Autoradiography; D046508:Culture Techniques; D006651:Histocytochemistry; D007124:Immunoenzyme Techniques; D008297:Male; D010903:Pituitary Gland, Anterior / Q000032:analysis* / Q000166:cytology; D011863:Radioimmunoassay / Q000379:methods*; D051381:Rats
D000324:Adrenocorticotropic Hormone