Determination of the isotope effect of the enzymatic oxidation of (R)Carnitine by displacement of the equilibrium via mass action.
An isotope effect of the dehydrogenation of (R) Carnitine [(R) 3-hydroxy-4-trimethylaminobutyric acid hydrochloride] catalyzed by (R) carnitine dehydrogenase [(R) carnitine: NAD oxidoreductase E.C. 1.1.1.108] from Pseudomonas aeruginosa was measured at different temperatures. It was found that k1H/k3H does not vary greatly with changes of temperature. The value of 3 for k1H/k3H measured at small initial conversions strongly indicated that the rate limiting step of the oxidation of (R) carnitine is the cleavage of the C-H bond at C3.
D000429:Alcohol Oxidoreductases / Q000378:metabolism; D002331:Carnitine / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009243:NAD / Q000378:metabolism; D011550:Pseudomonas aeruginosa; D013696:Temperature; D014316:Tritium / Q000494:pharmacology*
D009243:NAD; D014316:Tritium; D000429:Alcohol Oxidoreductases; D002331:Carnitine