Enzymes of ammonia assimilation in Rhizobium leguminosarum bacteroids.
The activities of the following enzymes were studied in connection with dinitrogen fixation in pea bacteroids: glutamine synthetase(L-glutamate: ammonia ligase (ADP-forming)(EC 6.3.1.2)(GS); glutamate dehydrogenase (NADP+)(L-glutamate: NADP+ oxidoreductase (deaminating)(EC 1.4.1.4)(GDH); glutamate synthase (L-glutamine: 2-exeglutarate aminotransferase (NADPH-oxidizing))(EC 2.6.1.53)(GOGAT). GS activity was high throughout the growth of the plant and GOGAT activity was always low. It is unlikely that GDH or the GS-GOGAT pathway can account for the incorporation of ammonia from dinitrogen fixation in the pea bacteroid,
D000641:Ammonia / Q000378:metabolism; D002474:Cell-Free System; D005969:Glutamate Dehydrogenase / Q000378:metabolism*; D005970:Glutamate Synthase / Q000378:metabolism*; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism*; D009586:Nitrogen Fixation; D009591:Nitrogenase / Q000378:metabolism; D063245:Plant Development; D010944:Plants / Q000382:microbiology*; D012231:Rhizobium / Q000201:enzymology* / Q000378:metabolism; D012988:Soil Microbiology; D000637:Transaminases / Q000378:metabolism*; D014675:Vegetables
D000641:Ammonia; D009591:Nitrogenase; D005970:Glutamate Synthase; D005969:Glutamate Dehydrogenase; D000637:Transaminases; D005974:Glutamate-Ammonia Ligase