Restoration of membrane excitability of squid giant axons by reagents activating tyrosine-tubulin ligase.
Using squid giant axon, an experimental survey was performed on restoration of the membrane excitability which had been partially suppressed. Among reagents examined, a combination of 400 mM KF, 50 micrometers tyrosine, 1 mM ATP, 1 mM Mg ions and 2 micrometers cAMP was found to induce the restoration of the excitability to a large extent. Further addition of a small amount of either porcine brain microtubule proteins or the squid axoplasm was found to support complete restoration. The experiments suggest that tubulin-tyrosine ligase contained in the porcine brain microtubule protein fraction or the squid axoplasm maintains the coupling between cytoskeletal structures and the plasma membrane.
D000200:Action Potentials / Q000187:drug effects; D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D001369:Axons / Q000502:physiology*; D002462:Cell Membrane / Q000187:drug effects; D000242:Cyclic AMP / Q000494:pharmacology; D049832:Decapodiformes; D008025:Ligases / Q000378:metabolism*; D008274:Magnesium / Q000494:pharmacology; D008564:Membrane Potentials / Q000187:drug effects; D008870:Microtubules / Q000187:drug effects; D009711:Nucleotides / Q000494:pharmacology; D014404:Tubulin / Q000494:pharmacology*; D014443:Tyrosine / Q000494:pharmacology*
D009711:Nucleotides; D014404:Tubulin; D014443:Tyrosine; D000255:Adenosine Triphosphate; D000242:Cyclic AMP; D008025:Ligases; D008274:Magnesium