Affinity labeling of the ribonucleic acid component adjacent to the peptidyl recognition center of peptidyl transferase in Escherichia coli ribosomes.
N-Iodacetylphenylalanyl-tRNA was used as an affinity label for localizing the RNA components intimately related to the peptidyl transferase activity of Escherichia coli ribosomesmthis analogue could specifically alkylate a unique nucleotide chain of 23-S RNA. The alkylation was strongly enhanced by poly(U), and was dependent on the presence of both 50- and 30-S subunits; Chloramphenicol inhibited the reaction, wheras blasticidin S stimulated it. The alkylated RNA base was found to be adenine. The nucleotide chain attacked by N-iodoacetylphenylalanyl-tRNA seemed to be localized at or near to the peptidyl recognition center of peptidyl transferase.
D000217:Acyltransferases / Q000378:metabolism*; D000345:Affinity Labels; D000478:Alkylation; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002499:Centrifugation, Density Gradient; D002701:Chloramphenicol / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007461:Iodoacetates; D009705:Nucleosides / Q000378:metabolism; D010458:Peptidyl Transferases / Q000378:metabolism*; D010649:Phenylalanine; D012335:RNA, Ribosomal / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism; D012260:Ribonucleases / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism*
D000345:Affinity Labels; D000900:Anti-Bacterial Agents; D007461:Iodoacetates; D009705:Nucleosides; D012335:RNA, Ribosomal; D010649:Phenylalanine; D002701:Chloramphenicol; C004500:blasticidin S; D012343:RNA, Transfer; D000217:Acyltransferases; D010458:Peptidyl Transferases; D012260:Ribonucleases