Cell surface changes accompanying viral transformation: N-acetylneuraminic acid ectotransferase system activity.
The activity of the sialyl ectotransferase system of normal chick embryo fibroblasts (CEF) and chick embryo fibroblasts transformed with the Schmidt-Ruppin strain of Rous sarcoma virus (SR-RSV) have been compared. Neuraminidase treatment of the intact cells increased the sialyl ectotransferase system activity of control and transformed cells two to three times. The ectotransferase system activity increased as the pH was decreased from 7.8 to 6.0. The temperature optimum for both systems was 40 degrees C. Approximately 60% of the 14C-sialic acid incorporated at pH 6.5 or above could be removed with neuraminidase. The activity of the transformed cell system with or without neuraminidase treatment was more stimulated by addition of Mn2+ ions, particularly above pH 7.0. This difference in ion sensitivity indicates that a different cell surface phenomenon is being studied after transformation.
D000123:Acetyltransferases / Q000378:metabolism*; D000818:Animals; D001358:Avian Sarcoma Viruses / Q000472:pathogenicity*; D002118:Calcium / Q000494:pharmacology; D002250:Carbon Radioisotopes; D002462:Cell Membrane / Q000201:enzymology*; D002642:Chick Embryo; D003588:Cytopathogenic Effect, Viral; D005347:Fibroblasts / Q000201:enzymology; D006863:Hydrogen-Ion Concentration; D008345:Manganese / Q000494:pharmacology; D009439:Neuraminidase / Q000378:metabolism; D011506:Proteins / Q000032:analysis; D012794:Sialic Acids / Q000378:metabolism; D012799:Sialyltransferases / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors; D014166:Transferases / Q000378:metabolism*
D002250:Carbon Radioisotopes; D011506:Proteins; D012794:Sialic Acids; D008345:Manganese; D014166:Transferases; D000123:Acetyltransferases; D012799:Sialyltransferases; D009439:Neuraminidase; D002118:Calcium