Isolation and properties of cytochrome c peroxidase from Pseudomonas denitrificans.
The isolation of cytochrome c peroxidase, cytochrome c4, cytochrome c-551 and azurin from Pseudomonas dentrificans is described. The peroxidase has a molecular weight of 63,000 and an isoelectric point of 5.6. Its absorption spectrum suggests that it contains two haem c groups/molecule. Preliminary steady-state kinetic data are reported with cytochromes c-551 and c4 and azurin as the second substrate.
D001400:Azurin / Q000302:isolation & purification; D002852:Chromatography, Ion Exchange; D003574:Cytochrome c Group / Q000302:isolation & purification; D003578:Cytochrome-c Peroxidase / Q000302:isolation & purification*; D007526:Isoelectric Point; D007700:Kinetics; D008970:Molecular Weight; D010544:Peroxidases / Q000302:isolation & purification*; D011549:Pseudomonas / Q000032:analysis / Q000201:enzymology*; D013053:Spectrophotometry
D003574:Cytochrome c Group; D001400:Azurin; D010544:Peroxidases; D003578:Cytochrome-c Peroxidase