A heat-stable protein inhibitor of cyclic 3',5'-nucleotide phosphodiesterase.
A heat-stable, non-dialyzable inhibitory factor of cyclic nucleotide phosphodieterase was detected in and partially purified from bovine retina. The factor appears to be a protein, since the inhibitory activity was abolished by trypsin digestion but not by DNAase or RNAase treatment. The protein inhibitor from bovine retina effectively inhibits the Ca2+-independent phosphodiesterase from several sources, including bovine retina, bovine rod outer segment, and a human lymphoblastic leukemia cell line, indicating lack of tissue and species specificity.
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000037:antagonists & inhibitors*; D000818:Animals; D001921:Brain / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D002417:Cattle; D002460:Cell Line; D004791:Enzyme Inhibitors / Q000302:isolation & purification; D006801:Humans; D007942:Leukemia, Experimental / Q000378:metabolism; D010786:Photoreceptor Cells / Q000378:metabolism; D011506:Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D012160:Retina / Q000378:metabolism
D004791:Enzyme Inhibitors; D011506:Proteins; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D002118:Calcium