title: Metabolism of carbohydrate derivatives by Pseudomonas acidovorans.
abstract: Wild-type Pseudomonas acidovorans strain A1 was unable to grow on glycerol or glucose as sole source of carbon and energy although it grew well on gluconate. Spontaneous glycerol-positive mutants, which apparently had become permeable to glycerol, were readily isolated, but glucose-positive mutants did not occur. P. acidovorans lacked glucose dehydrogenase and glucokinase, which were sufficient to account for its inability to grow on glucose. Gluconate was degraded exclusively via a noncoordinately induced Entner-Doudoroff pathway. Phosphogluconate dehydrogenase was undetectable. In contrast to P. aeruginosa, P. acidovorans possessed a single glyceraldehyde-phosphate dehydrogenase activity, which was NAD+ specific and constitutive, and an inducible pyruvate kinase. Moreover, growth of glycerol-positive strain K2 on glycerol did not induce any of the enzymes related to metabolism of hexosephosphate derivatives as occurs in fluorescent pseudomonads.
mesh_terms: D005942:Gluconates / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D009154:Mutation; D009243:NAD / Q000378:metabolism; D010735:Phosphoglycerate Kinase / Q000378:metabolism; D010736:Phosphoglycerate Mutase / Q000378:metabolism; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D011549:Pseudomonas / Q000235:genetics / Q000378:metabolism*; D011770:Pyruvate Kinase / Q000378:metabolism
chemical_list: D005942:Gluconates; D009243:NAD; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D011770:Pyruvate Kinase; D010735:Phosphoglycerate Kinase; D010751:Phosphopyruvate Hydratase; D010736:Phosphoglycerate Mutase; D005947:Glucose; D005990:Glycerol