title: Gene duplication as a mechanism of genetic adaptation in Saccharomyces cerevisiae.
abstract: It has been shown that specific mutations of the gene that codes for the general acid monophophatase (Aphtase) of S. cerevisiae can increase the affinity of this enzyme for beta-glycerophosphate (BGP) and thereby provide this organism with the capacity to exploit extremely low concentrations of this organic phosphate (Francis and Hansche 1973). In this report two additional avenues are demonstrated to be available to this organism for increasing its capacity to exploit low concentrations of organic phosphates. One avenue is through mutations that increase the amount of Aphtase that associates with the cell wall, where it catalizes the hydrolysis of exogenous organic phosphates. The other avenue is through duplication of the gene that codes for Aphtase, doubling the amount of Aphtase synthesized.--The spontaneous duplication of the structural gene of Aphtase and the incorporation of the duplicate into this experimental population as a means of exploiting low concentrations of exogenous organic phosphates provides direct support for the first step of the mechanism through which new metabolic functions are postulated to evolve.
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D002473:Cell Wall / Q000201:enzymology; D002869:Chromosome Aberrations; D005796:Genes; D005838:Genotype; D006863:Hydrogen-Ion Concentration; D009154:Mutation; D009943:Organophosphorus Compounds / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000378:metabolism; D012641:Selection, Genetic; D013172:Spores, Fungal
chemical_list: D009943:Organophosphorus Compounds; D000135:Acid Phosphatase