title: CO2 fixation and its regulation in Anacystis nidulans (Synechococcus).
abstract: Anacystis nidulans (Synechococcus) had a minimal doubling time of 5 hrs at 30 degrees C at saturating light intensity and carbon dioxide concentration. Half maximal growth rates in saturating CO2 occured at a light intensity of 0.54 mW per cm2, and there was an apparent threshold intensity of 0.13 mW per cm2 below which no growth occurred. Growth rate in saturating light was dependent on the concentration of CO2+H2CO3 in the medium, rather than on total dissolved CO2; half maximal rates were estimated at 0.1 mM CO2+H2CO3. Under saturating conditions of light and CO2, 14CO2 was fixed primarily into 3-PGA, and subsequently moved into sugar phosphates and amino acids. Incorporation into aspartate was relatively slow. CO2 fixation was strictly light-dependent. The changes in adenylate and pyridine nucleotide pools were followed in light/dark and dark/light transitions. Whereas adenylates relaxed slowly over 15-20 min to the concentrations characteristic of illuminated cells following the abrupt changes induced by darkening, the sharp drop in intracellular NADPH showed little dark recovery although rapid restoration occurred on reillumination. Other pyridine nucleotides showed no changes during these transitions. The nucleotide specificity and Km of partially purfied GAP dehydrogenase suggest a role for this enzyme in the regulation of CO2 fixation.
mesh_terms: D000249:Adenosine Monophosphate / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D001224:Aspartic Acid / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism*; D002250:Carbon Radioisotopes; D002255:Carbonic Acid; D003470:Culture Media; D000458:Cyanobacteria / Q000378:metabolism*; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism; D008027:Light; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010428:Pentosephosphates / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D010761:Phosphorus Radioisotopes; D013045:Species Specificity; D013403:Sugar Phosphates / Q000378:metabolism
chemical_list: D000596:Amino Acids; D002250:Carbon Radioisotopes; D003470:Culture Media; D010428:Pentosephosphates; D010710:Phosphates; D010761:Phosphorus Radioisotopes; D013403:Sugar Phosphates; D009243:NAD; D002245:Carbon Dioxide; D001224:Aspartic Acid; D000249:Adenosine Monophosphate; D002255:Carbonic Acid; D009249:NADP; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases