Factors affecting the survival of sheep embryos during-freezing and thawing.
The survival of sheep morulae and early blastocysts after deep freezing has been investigated. With a dimethyl sulphoxide concentration of 1.5 M in a phosphate-buffered saline medium, a freezing rate of 1 degreees C/min down to 120 degees C allowed embryos to survival only if rapid thawing (approximately 360 degrees C/min) was employed, whereas with a freezing rate of 0.3 degrees C/min, survival was obtained with both rapid and slow (10 degrees C or 4 degrees C/min) thawing. With even slower freezing (0.1 degrees C/min over intervals between -30 and -60 degrees C) slow thawing was no longer optional but mandatory. With the latter technique survival rates of the order of 80% were obtained. The results of transfer experiments with frozen-thawed sheep and cattle embryos are briefly presented in an Appendix.
D000818:Animals; D001755:Blastocyst / Q000166:cytology; D002417:Cattle / Q000196:embryology; D004121:Dimethyl Sulfoxide; D004477:Ectogenesis; D004622:Embryo, Mammalian / Q000502:physiology; D005615:Freezing; D006358:Hot Temperature; D011309:Preservation, Biological / Q000379:methods*; D012756:Sheep / Q000196:embryology; D014022:Tissue Survival
D004121:Dimethyl Sulfoxide