Response of hemopoietic cells to avian acute leukemia viruses: effects on the differentiation of the target cells.
Chicken bone marrow cells were infected with three avian acute leukemia viruses (ALV)--avian myeloblastosis virus (AMV), myelocytomatosis virus strain MC29 and Mill Hill 2 virus (MH2)--and then cultured in agar in the presence of conditioned medium. Under these conditions, it was found that very few cells served as target cells for these three viruses. Density gradient separation showed that ALV target cells were found primarily in the light density fractions and might be represented by cells committed to the mononuclear phagocyte pathway. Separation of bone marrow cells on the basis of their sedimentation velocity at unit gravity suggested that MC29 and AMV did not share the same target cells. In addition, the analysis of surface receptors and functional markers characteristic of macrophages (Fc and complement receptors, phagocytosis and immune phagocytosis) indicated that the ALV-transformed cells were blocked during their differentiation. These results indicate that the transforming ability of ALV interferes with the differentiation of their target cells.
D001354:Avian Leukosis Virus; D001665:Binding Sites; D001853:Bone Marrow / Q000382:microbiology; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods; D002472:Cell Transformation, Viral; D002999:Clone Cells / Q000502:physiology; D003176:Complement C3; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000382:microbiology; D007141:Immunoglobulin Fc Fragments; D008264:Macrophages / Q000382:microbiology; D010587:Phagocytosis
D003176:Complement C3; D007141:Immunoglobulin Fc Fragments