Difference in renal alpha-galactosidase levels in male and female Chinese hamsters (Cricetulus griseus).
1. The activity of alpha-galactosidase was found to be significantly higher in the kidney of female than that of male Chinese hamsters in a highly inbred colony but its activity in liver, heart and spleen remained similar between female and male animals. 2. Partially purified renal alpha-galactosidase by sequential column chromatography on Sepharose 6B and DEAE-Sepharose CL-6B showed identical elution profiles, pH optima (4.5), KmS (4.4 mM) and heat-inactivation curves between enzymes of male and female animals. 3. Thus, the observed higher activity of renal alpha-galactosidase in the females was due to elevated enzyme concentration, not a result of enzyme polymorphism.
D000818:Animals; D006224:Cricetinae; D003412:Cricetulus; D005260:Female; D005696:Galactosidases / Q000378:metabolism*; D007668:Kidney / Q000201:enzymology*; D008297:Male; D000519:alpha-Galactosidase / Q000378:metabolism*
D005696:Galactosidases; D000519:alpha-Galactosidase