An avidin-biotinyl-propranolol complex for beta-adrenergic receptor characterization.
The synthesis of biotinyl-hexaglycyl-NEDA (abbreviation:BGN), a biotinyl derivative of propranolol, is described. This bifunctional molecule binds with high affinity to the biotin-binding protein, avidin. The duck erythrocyte was used as a model beta-receptor system. Formation of an avidin-BGN-beta-receptor complex was demonstrated in intact erythrocytes, in erythrocyte ghosts, and in the digitonin-solubilized beta-receptor. The avidin-BGN complex will be used for localization and purification of the beta-receptor.
D000818:Animals; D001360:Avidin / Q000378:metabolism*; D001710:Biotin / Q000031:analogs & derivatives* / Q000378:metabolism; D004372:Ducks; D004910:Erythrocyte Membrane / Q000032:analysis* / Q000648:ultrastructure; D004912:Erythrocytes / Q000032:analysis*; D007700:Kinetics; D010047:Ovalbumin / Q000031:analogs & derivatives*; D011433:Propranolol / Q000031:analogs & derivatives*; D011941:Receptors, Adrenergic / Q000302:isolation & purification*; D011943:Receptors, Adrenergic, beta / Q000302:isolation & purification* / Q000378:metabolism; D012995:Solubility
D011941:Receptors, Adrenergic; D011943:Receptors, Adrenergic, beta; D001360:Avidin; D001710:Biotin; D010047:Ovalbumin; D011433:Propranolol