Identification of a new DNA polymerase activity in human KB cells.
We have isolated and partially characterized a new DNA polymerase activity from the purified nuclear fraction of cultured human KB cells. The physical and chemical properties of this enzyme which we have designated DNA polymerase N3 define it as a unique entity that can be distinguished from the several classes of eukaryotic D-DNA and R-DNA polymerase activity that have been recognized to date.
D002460:Cell Line; D002467:Cell Nucleus / Q000201:enzymology; D002848:Chromatography, DEAE-Cellulose; D004254:DNA Nucleotidyltransferases / Q000302:isolation & purification* / Q000378:metabolism; D005092:Exonucleases / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006902:Hydroxymercuribenzoates / Q000494:pharmacology; D007525:Isoelectric Focusing; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D008928:Mitochondria / Q000378:metabolism; D008970:Molecular Weight; D009062:Mouth Neoplasms; D011189:Potassium Chloride / Q000494:pharmacology; D013698:Templates, Genetic
D006902:Hydroxymercuribenzoates; D011189:Potassium Chloride; D004254:DNA Nucleotidyltransferases; D005092:Exonucleases; D008274:Magnesium