[Passive hemagglutination test for individual or epidemiologic diagnosis of human brucellosis].
Coupling of an extract from B. abortus strain 99 to sheep red blood cells by chromium chloride allowed to develop a highly specific and reliable passive hemagglutination test (H). Indeed, H was positive in 99.8 per cent sera from 968 known Brucella-infected heifers, while tube agglutination revealed only 59 per cent and complement fixation 90.9 per cent positive cases. H was positive in 153 sera from 778 proven or suspected human brucellosis where agglutination and/or complement fixation tests were positive, and in 21 sera negative to conventional tests. H demonstreated remote or undiagnosed human B. abortus infections in 28.6 per cent of 18 367 human sera randomly examined in a rural area. Therefore, a simple easy-to-perform passive hemagglutination test could replace a battery of conventional tests for the diagnosis of individual brucellosis, and epidemiologic surveys. The present study points to a high prevalence of undiagnosed brucellosis in man, specially in those area where cattle are raised.
D000818:Animals; D002003:Brucella abortus / Q000276:immunology; D002006:Brucellosis / Q000175:diagnosis* / Q000453:epidemiology / Q000517:prevention & control; D002007:Brucellosis, Bovine / Q000175:diagnosis; D002417:Cattle; D003168:Complement Fixation Tests; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D005602:France; D006386:Hemagglutination Tests / Q000379:methods*; D006801:Humans; D008297:Male; D009790:Occupations; D012756:Sheep / Q000276:immunology
Hémagglutination passive appliquée au diagnostic individuel ou épidémiologique de la brucellose humaine.