Control of amylase biosynthesis and release in the parotid gland of the rat.
1. Amylase biosynthesis and release in the rat parotid were studied under various conditions. Incorporation of [(3)H]leucine into amylase, extracted from the tissue by immunoadsorbent, was measured and found to be time-dependent and totally inhibited by the protein synthesis inhibitor puromycin. 2. Adrenaline, at a concentration (10mum) that gave maximum stimulation of release, inhibited [(3)H]leucine incorporation into both total protein and amylase. This effect was reversed by phentolamine. 3. Adrenaline (1mum) and isoproterenol (10mum) stimulated biosynthesis of total protein and amylase. These effects were blocked by propranolol, as were the effects on release. Dibutyryl cyclic AMP (2mm) mimicked the effects of isoproterenol and adrenaline (1mum) on both amylase biosynthesis and release. All the above stimulatory effects on amylase biosynthesis were only observed if the tissue was pretreated with effector before pulse-labelling with [(3)H]leucine. 4. Insulin (625muunits/ml initial concentration, 150muunits/ml final concentration) stimulated incorporation of [(3)H]leucine into total protein and amylase when added to the tissue at the same time as the leucine. 5. Carbamoylcholine (10mum) decreased [(3)H]leucine incorporation into total protein and amylase when both were added to the tissue simultaneously, but this effect was prevented by removal of effector and washing the tissue before addition of [(3)H]leucine. 6. Stimulation of beta-adrenergic receptors increased both amylase release and biosynthesis, but stimulation of alpha-receptors can inhibit biosynthesis without inhibiting release. Cholinergic agents can also inhibit amylase biosynthesis, but stimulate release. Insulin at approximately physiological concentration can increase incorporation of leucine into amylase without stimulating release. The system described therefore provides an excellent model for the further investigation of the mechanisms of these diverse effects.
D000681:Amylases / Q000096:biosynthesis* / Q000276:immunology; D000818:Animals; D003994:Bucladesine / Q000494:pharmacology; D004837:Epinephrine / Q000494:pharmacology; D007164:Immunosorbents; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology; D007930:Leucine / Q000378:metabolism; D008297:Male; D010306:Parotid Gland / Q000187:drug effects / Q000201:enzymology*; D051381:Rats; D011941:Receptors, Adrenergic / Q000187:drug effects
D007164:Immunosorbents; D007328:Insulin; D011941:Receptors, Adrenergic; D003994:Bucladesine; D000681:Amylases; D007930:Leucine; D004837:Epinephrine