Isolation and development of a reticuloendotheliosis virus-transformed lymphoblastoid cell line from chicken spleen cells.
The establishment and characterization of a reticuloendotheliosis virus (strain T)-transformed lymphoblastoid cell line, designated TV-1, was reported. These cells, isolated from the spleen of a moribund chick infected with reticuloendotheliosis virus, were maintained in suspension culture for over 74 weeks at a stable generation time of 15 h. The cells were found to carry a female karyotype. Both TV-1 cells and cell-free TV-1 culture supernatant produced lesions and mortality patterns in chicks which were identical to those caused by reticuloendotheliosis virus (strain T) infection. Examination of TV-1 cells by electron microscope revealed the presence of C-type virions budding from the plasma membrane. Cytotoxicity assays and fluorescent antibody tests indicated the presence of B-cell determinants on the TV-1 cell surface membrane.
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis; D002455:Cell Division; D002460:Cell Line; D002472:Cell Transformation, Viral; D002645:Chickens; D003601:Cytotoxicity Tests, Immunologic; D007621:Karyotyping; D008214:Lymphocytes / Q000382:microbiology; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D001356:Reticuloendotheliosis virus; D012190:Retroviridae; D013154:Spleen / Q000382:microbiology
D000954:Antigens, Surface; D000956:Antigens, Viral; D011947:Receptors, Antigen, B-Cell