title: Local aggregation of hormone-receptor complexes is required for activation by epidermal growth factor.
abstract: An analogue of epidermal growth factor (EGF) which is virtually devoid of biological activity retains receptor binding activity but cannot form cell surface clusters or patches. Bivalent anti-EGF antibodies restore both bioactivity and patch formation. The sensitivity of fibroblasts to native EGF can also be enhanced greatly by these antibodies, especially in hormone-resistant cell lines.
mesh_terms: D000818:Animals; D002460:Cell Line; D003488:Cyanogen Bromide; D004815:Epidermal Growth Factor / Q000276:immunology / Q000378:metabolism*; D007152:Immunologic Capping; D007158:Immunologic Techniques; D008560:Membrane Fluidity; D051379:Mice; D008856:Microscopy, Fluorescence; D010446:Peptide Fragments / Q000378:metabolism; D010455:Peptides / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D013329:Structure-Activity Relationship
chemical_list: D010446:Peptide Fragments; D010455:Peptides; D011956:Receptors, Cell Surface; D004815:Epidermal Growth Factor; D003488:Cyanogen Bromide