A procedure for the rapid lysis of mammalian cells prior to alkaline sucrose density gradient centrifugation.
D002458:Cell Fractionation / Q000379:methods*; D002460:Cell Line; D002477:Cells / Q000648:ultrastructure*; D002499:Centrifugation, Density Gradient; D006863:Hydrogen-Ion Concentration; D013696:Temperature; D013997:Time Factors