Lactate-to-pyruvate or pyruvate-to-lactate assay for lactate dehydrogenase: a re-examination.
The pyruvate-to-lactate assay for determining lactate dehydrogenase (EC 1.1.1.27) can now yield linearity equal to or better than that obtained by the lactate-to-pyruvate assay. In addition, there are significant advantages to the pyruvate-to-lactate reaction: (a) a greater change in absorbance per unit time, which allows more accurate spectrophotometric readout; (b) lower reactant concentrations are required, which substantially reduces the cost per assay; (c) solid reagents are used to prepare the assay solution; and (d) reagent solutions are more stable. However, impurities present in commercial NADH preparations may substantially affect measured lactated dehydrogenase activities; therefore, a Standard Reference Material for NADH is being developed for issuance by the National Bureau of Standards.
D007770:L-Lactate Dehydrogenase / Q000097:blood*; D007773:Lactates / Q000032:analysis / Q000378:metabolism*; D009243:NAD / Q000592:standards; D010084:Oxidation-Reduction; D011773:Pyruvates / Q000032:analysis / Q000378:metabolism*; D012015:Reference Standards; D013053:Spectrophotometry
D007773:Lactates; D011773:Pyruvates; D009243:NAD; D007770:L-Lactate Dehydrogenase