The isolation and cultivation of calf rotavirus in the Republic of South Africa.
Calf rotavirus was cultivated and propagated in tissue culture from faeces of 3-week-old calves suffering from severe diarrhoea. Criteria for viral involvement were: production of cytopathic effect in primary foetal calf kidney cells, specific fluorescence, and identification of the agent by means of electron microscopy. In a limited serological survey the majority of the cows on an infested farm were found to possess neutralizing antibodies to the local rotavirus strain.
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology*; D003588:Cytopathogenic Effect, Viral; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary*; D005455:Fluorescent Antibody Technique; D007668:Kidney; D012087:Reoviridae / Q000302:isolation & purification*; D012401:Rotavirus / Q000302:isolation & purification* / Q000648:ultrastructure; D013019:South Africa; D014776:Virus Cultivation / Q000379:methods