An attempt to analyze various thyroid stimulators by the receptor assay using hTSH radioimmunoassay.
In an attempt to analyze thyroid stimulators in serum we developed an assay procedure using hTSH radioimmunoassay (RIA) in combination with receptor competition. The principle of this method is the determination by RIA of hTSH displaced by other thyroid stimulators from a thyroidal receptor preparation which previously bound unlabelled hTSH. Practically 4 microunits of hTSH were bound with human or bovine receptor, and then hTSH displaced by addition of test serum (0.1 ml) or samples dissolved in serum (0.1 ml) was measured by RIA. This assay can determine the thyroid stimulators other than hTSH in serum that has the displacement activity of 0.5-4.0 microunits of hTSH in the useful range, such as mU/ml level of bovine TSH or rat TSH. Cholera toxin that has the thyroid stimulating activity like TSH also showed the displacement of the bound hTSH. This assay is not applicable for the human serum with more than 5 microunits/ml of TSH, because the assay value is over estimated by the free hTSH derived from the test serum. On the other hand, eighteen sera with high LATS activity and 42 sera with negative LATS activity from patients with untreated hyperthyroidism did not show any displacement. This might be due to the lower binding activity of LATS with hTSH receptor or the lower sensitivity of this assay method. Although it is difficult to use this assay clinically because of its low sensitivity, increased TSH in animal serum can be determined by this assay. The principle of this method may be also useful for examining the receptor binding of other peptide hormone that can be determined by an RIA method.
D000818:Animals; D001667:Binding, Competitive; D002417:Cattle; D002772:Cholera Toxin / Q000032:analysis; D006801:Humans; D006980:Hyperthyroidism / Q000097:blood; D011863:Radioimmunoassay / Q000379:methods*; D011869:Radioligand Assay / Q000379:methods*; D051381:Rats; D011956:Receptors, Cell Surface / Q000032:analysis; D013961:Thyroid Gland / Q000032:analysis; D013972:Thyrotropin / Q000097:blood*
D011956:Receptors, Cell Surface; D013972:Thyrotropin; D002772:Cholera Toxin