Specific conversion of s4 U to U in Escherichia coli tRNA by iodate oxidation.
The minor nucleoside 4-thiouridine in Escherichia coli tRNA is transformed selectively to uridine by iodate oxidation at acidic pH. The four major nucleotides were found to be inert under these conditions. The iodate oxidation appears to be more specific than the previous conversion methods reported, and has the advantage that it does not affect the chargeability of most tRNA.
D004926:Escherichia coli / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D007452:Iodates; D009682:Magnetic Resonance Spectroscopy; D010084:Oxidation-Reduction; D012329:RNA, Bacterial; D012343:RNA, Transfer; D013056:Spectrophotometry, Ultraviolet; D013891:Thiouridine / Q000032:analysis
D007452:Iodates; D012329:RNA, Bacterial; D013891:Thiouridine; D012343:RNA, Transfer