Apoenzyme of aspartate aminotransferase isozymes in serum and its diagnostic usefullness for hepatic diseases.
Aspartate aminotransferase in the sera of normal subjects and of patients with hepatic diseases has been immunologically separated into two isoenzymes, cytosolic aspartate aminotransferase and mitochondrial aspartate aminotransferase. The activity of the isoenzymes was measured in three different buffer solutions with or without pyridoxal 5'-phosphate. To attain maximal activation, the apoenzyme of mitochondrial fraction must be preincubated with pyridoxal 5'-phosphate longer than that of the cytosolic fraction in either of the three reaction mixtures. In most sera the activity of both isoenzymes increased substantially in the presence of pyridoxal 5'-phosphate regardless of the type of buffer solutions. Both the apoenzymatic activity and the ratio of apo- to holo-enzymatic activity of each of the isoenzymes varied among samples from the patients with hepatic diseases. However, significantly high ratios of apo- to holo-enzymatic activity of both isoenzymes were observed in the patients with hepatoma in contrast with those with other hepatic diseases. These findings suggest that the simultaneous measurement of both apo- and holo-enzyme activities of aspartate aminotransferase isoenzymes may be useful in the clinical assessment of hepatic diseases.
D001051:Apoenzymes / Q000097:blood; D001219:Aspartate Aminotransferases / Q000097:blood*; D006528:Carcinoma, Hepatocellular / Q000201:enzymology; D003600:Cytosol / Q000201:enzymology; D003937:Diagnosis, Differential; D006505:Hepatitis / Q000201:enzymology; D006801:Humans; D007527:Isoenzymes / Q000097:blood*; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D008103:Liver Cirrhosis / Q000201:enzymology; D008107:Liver Diseases / Q000175:diagnosis* / Q000201:enzymology; D008113:Liver Neoplasms / Q000201:enzymology; D008930:Mitochondria, Liver / Q000201:enzymology
D001051:Apoenzymes; D007527:Isoenzymes; D001219:Aspartate Aminotransferases