Evaluation of bacterial contamination in blood processing.
In the routine deglycerolization of frozen red blood cells, a bottle of solution intended as a final wash (0.8% NaCl, 0.2% dextrose) was found to be abnormally colored and slightly turbid. Close inspection revealed a small crack in the base of the bottle. A pure growth of Klebsiella pneumoniae was cultured. The rabbit pyrogen test on the solution was positive using 2.5 mul as was the limulus amebocyte lysate (LAL) test at a dilution of 1:10-5 for endotoxin. Studies of the growth of this organism are described and the clinical implications are discussed.
D000818:Animals; D001431:Bacteriological Techniques; D001681:Biological Assay; D001771:Blood Banks; D003445:Crustacea; D003470:Culture Media; D004340:Drug Contamination; D004731:Endotoxins / Q000032:analysis; D005069:Evaluation Studies as Topic; D005334:Fever; D005947:Glucose; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007711:Klebsiella pneumoniae / Q000254:growth & development / Q000302:isolation & purification*; D011749:Pyrogens; D011817:Rabbits; D012965:Sodium Chloride; D012996:Solutions / Q000032:analysis
D003470:Culture Media; D004731:Endotoxins; D011749:Pyrogens; D012996:Solutions; D012965:Sodium Chloride; D005947:Glucose