Influence of albumin on rubella virus hemagglutination and the hemagglutination-inhibition test.
The HEPES-saline-albumin-gelatin (HSAG) diluent found optimal for agglutination of fowl erythrocytes by rubella virus antigen is also optimal for agglutination of trypsin-treated human group O cells. Albumins from different commercial sources, however, can have varying inhibitory effects on rubella hemagglutination titers. This can have a significant effect on the hemagglutination-inhibition test since antibody titers measured by this procedure are related to the amount of antigen used.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D000956:Antigens, Viral; D002645:Chickens / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology; D005069:Evaluation Studies as Topic; D005780:Gelatin / Q000494:pharmacology; D006385:Hemagglutination Inhibition Tests / Q000379:methods*; D006386:Hemagglutination Tests / Q000379:methods*; D006387:Hemagglutination, Viral / Q000187:drug effects*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D012409:Rubella / Q000175:diagnosis; D012412:Rubella virus / Q000276:immunology*; D012710:Serum Albumin, Bovine / Q000494:pharmacology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D012710:Serum Albumin, Bovine; D005780:Gelatin