Ligand binding to ferrocytochrome c at high pH.
Ferrocytochrome c has been shown to bind two molecules of CO at pH 14. The second CO is thought to be bound only when the cytochrome c molecule is denatured, and once bound appears to be spectrally silent. Insolubilization of native cytochrome c prevents the binding of the second CO molecule. A scheme is proposed to explain these observations based on evidence from static titrations and flash-photolysis experiments, use of carboxymethyl cytochrome c and insoluble cytochrome c, and use of cyanide instead of CO as a ligand.
D002248:Carbon Monoxide; D003486:Cyanides; D003574:Cytochrome c Group; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008024:Ligands; D010782:Photolysis; D011485:Protein Binding
D003486:Cyanides; D003574:Cytochrome c Group; D008024:Ligands; D002248:Carbon Monoxide