title: Subcellular distribution and properties of kynurenine pyruvate transaminase in rat kidney.
abstract: Kynurenine transaminase activity in rat kidney was found in both the mitochondrial and supernatant fractions. These fractions contained (a) kynurenine pyruvate transaminase, which showed a preference for pyruvate as amino acceptor, and had a pH optimum between 8.0 and 8.5, and (b) kynurenine 2-oxoglutarate transaminase, with a preference for 2-oxoglutarate and a pH optimum between 6.0 and 6.5. The apparent Km value of the former enzyme for L-kynurenine was much lower than that of the latter enzyme.
mesh_terms: D003600:Cytosol / Q000201:enzymology; D006863:Hydrogen-Ion Concentration; D007656:Ketoglutaric Acids; D007668:Kidney / Q000201:enzymology*; D007700:Kinetics; D007737:Kynurenine; D008930:Mitochondria, Liver / Q000201:enzymology; D011773:Pyruvates; D013347:Subcellular Fractions / Q000201:enzymology; D000637:Transaminases / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D007656:Ketoglutaric Acids; D011773:Pyruvates; D007737:Kynurenine; D000637:Transaminases