Photokinetic microassay of adenylate kinase using the firefly luciferase reaction.
A new rapid photokinetic method is described for determining the activity of adenylate kinase (ATP:AMP phosphotranspherase, EC 2.7.4.3) in 0.1--5.0 micrograms of freeze-dried tissue. This represents a weight range far below that obtainable by fine-needle biopsy. The reaction 2 ADP in equilibrium with AMP + ATP was employed and the ATP formed assayed with firefly luciferase as light yielder. The light emission was recorded on a multi-channel scaler. The adenylate kinase activities found in tissues of mice were in the same range as previously described in a study using fluorometric microassay.
D000263:Adenylate Kinase / Q000032:analysis*; D000818:Animals; D001517:Coleoptera / Q000201:enzymology; D005260:Female; D006943:Hyperglycemia / Q000201:enzymology; D007515:Islets of Langerhans / Q000201:enzymology; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D008156:Luciferases / Q000378:metabolism*; D008163:Luminescent Measurements; D008722:Methods; D051379:Mice; D008820:Mice, Obese; D008832:Microchemistry; D010770:Phosphotransferases / Q000032:analysis*
D008156:Luciferases; D010770:Phosphotransferases; D000263:Adenylate Kinase