[Antiviral activity of interferon and its inducers in human lymphoblastoid and somatic cells].
The antiviral effect of interferon inductors, such as poly-I--poly-C, phage f2 RNA replicative form and low molecular inductor GSN and their influence on cellular DNA synthesis were studied in the cultures of lymphoblastoid (inplanting lines Raji Namalva) and somatic human cells. The Semliki forest virus used as the test organism multiplicated well in cells Raji accumulating up to 9 lg BOU/ml. The two-strand RNA was less active in the lymphoid cells than in the somatic ones. GSN was 10 times more active and less toxic in cells Raji as compared to the fibroblasts. The lymphoblastoid interferon had higher antiviral activity as compared to the fibroblast interferon in the system of Raji--Semliki forest virus than in the system of the human embryon fibroblast--Venezuela Horse Encephalytic Virus. Romantadin actively inhibited (100 times) production of the alfavirus in both the somatic and lymphoblastoid cells.
D000218:Adamantane / Q000031:analogs & derivatives / Q000494:pharmacology; D000998:Antiviral Agents; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004666:Encephalitis Virus, Venezuelan Equine / Q000187:drug effects; D005347:Fibroblasts / Q000382:microbiology; D004854:Herpesvirus 4, Human / Q000187:drug effects; D006801:Humans; D066298:In Vitro Techniques; D007369:Interferon Inducers / Q000494:pharmacology*; D007372:Interferons / Q000494:pharmacology*; D008214:Lymphocytes / Q000187:drug effects*; D012313:RNA / Q000096:biosynthesis; D012672:Semliki forest virus / Q000187:drug effects; D012845:Sindbis Virus / Q000187:drug effects; D014776:Virus Cultivation; D014779:Virus Replication / Q000187:drug effects
D000998:Antiviral Agents; D007369:Interferon Inducers; D012313:RNA; D004247:DNA; D007372:Interferons; D000218:Adamantane
Protivovirusnaia aktivnost' interferona i ego induktorov v limfoblastoidnykh i somaticheskikh kletkakh cheloveka.