title: [Studies on the stearic acid dehydrogenase in the liver and brain of rats of various ages (author's transl)].
abstract: Tissue slices from the liver and brain of 7-day-old rats incubated with [1-14C]stearic acid desaturate the stearate to oleate. The activities of the two tissues are different but of the same order of magnitude. With increasing age, the activity in the liver increases markedly, while the brain activity decreases. The postmitochondrial supernatant from adult (3-month-old) liver contains 2 to 3 orders of magnitude more stearoyl-CoA dehydrogenase activity than the brain postmitochondrial fraction. The washed microsomal fraction from liver had about the same activity as the postmitochondrial supernatant, but no dehydrogenase activity could be detected in the washed microsomal fraction from the brain. The acyl-CoA synthetase and the palmitoyl-CoA hydrolase activities measured in the washed microsomes from adult brain were both lower than in liver microsomes. The concentration of stearoyl-CoA (the substrate for the stearoyl-CoA dehydrogenase) resulting from the ratio of these activities was too high, however, for the lack of desaturase activity to have been simulated by lack of substrate.
mesh_terms: D000375:Aging; D000818:Animals; D001921:Brain / Q000201:enzymology* / Q000254:growth & development; D004229:Dithiothreitol / Q000494:pharmacology; D044943:Fatty Acid Desaturases / Q000378:metabolism*; D008099:Liver / Q000201:enzymology* / Q000254:growth & development; D008861:Microsomes / Q000187:drug effects / Q000201:enzymology; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology; D008928:Mitochondria / Q000201:enzymology; D008930:Mitochondria, Liver / Q000201:enzymology; D009928:Organ Specificity; D051381:Rats; D013230:Stearoyl-CoA Desaturase / Q000378:metabolism*
chemical_list: D044943:Fatty Acid Desaturases; D013230:Stearoyl-CoA Desaturase; D004229:Dithiothreitol
vernacular_title: Untersuchungen zur Stearinsäure-Dehydrogenase in leber und Gehirn von Ratten verschiedenen Alters