Synthesis of yeast wall glucan.
Saccharomyces cerevisiae was treated with a mixture of toluene and ethanol to make it permeable to small molecules. This treatment unmasked a glucan synthetase activity which was assayed with UDP-[U-14C]glucose. About 60% of the polymer formed was beta-(I leads to 3)glucan. No labelled lipids were detected. The 14C incorporated was recovered in a particulate membrane preparation isolated by differential centrifugation. When the particles themselves were assayed for glucosyl transfer activity none was found. The toluene-treated preparations also catalysed the transfer of mannosyl residues from GDP-mannose to polymeric materials by a process independent of glucosyl transfer.
D002250:Carbon Radioisotopes; D002473:Cell Wall / Q000378:metabolism / Q000648:ultrastructure; D005947:Glucose / Q000378:metabolism; D006155:Guanosine Diphosphate Mannose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000494:pharmacology; D008358:Mannose / Q000378:metabolism; D008623:Mercaptoethanol / Q000494:pharmacology; D011134:Polysaccharides / Q000096:biosynthesis*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D014050:Toluene / Q000494:pharmacology; D014532:Uridine Diphosphate Glucose / Q000378:metabolism
D002250:Carbon Radioisotopes; D011134:Polysaccharides; D006155:Guanosine Diphosphate Mannose; D014050:Toluene; D008623:Mercaptoethanol; D008274:Magnesium; D005947:Glucose; D008358:Mannose; D014532:Uridine Diphosphate Glucose