The beta-galactosidase-catalyzed hydrolysis of o-nitrophenol-beta-D-galactoside at subzero temperatures: evidence for a galactosyl-enzyme intermediate.
D003080:Cold Temperature; D004121:Dimethyl Sulfoxide; D004926:Escherichia coli / Q000201:enzymology; D005696:Galactosidases / Q000378:metabolism*; D006027:Glycosides / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D009598:Nitrophenylgalactosides / Q000378:metabolism*
D006027:Glycosides; D009598:Nitrophenylgalactosides; D005696:Galactosidases; D008274:Magnesium; D004121:Dimethyl Sulfoxide