The preparation of stable enzyme-coenzyme complexes with endogenous catalytic activity.
N6-(6-Aminohexylcarbamoylmethyl)-NAD+ was coupled to lactate dehydrogenase by using glutaraldehyde to form an active complex. The stability of this complex could be considerably improved by reduction with KBH4, although this treatment caused a partial decrease in specific activity. NAD+ was also coupled directly to the enzyme by this method. All of these complexes exhibited an intrinsic activity in the absence of exogenous NAD+.
D001894:Borohydrides; D055598:Chemical Phenomena; D002621:Chemistry; D005976:Glutaral; D007770:L-Lactate Dehydrogenase; D046911:Macromolecular Substances; D009097:Multienzyme Complexes / Q000378:metabolism; D009243:NAD
D001894:Borohydrides; D046911:Macromolecular Substances; D009097:Multienzyme Complexes; D009243:NAD; D007770:L-Lactate Dehydrogenase; D005976:Glutaral