title: Reduction of ferricytochrome c, methemoglobin and metmyoglobin by hydroxyl and alcohol radicals.
abstract: We have studied the reaction of ferricytochrome c, methemoglobin and metmyoglobin with OH and alcohol radicals (methanol, ethanol, ethylene glycol and glycerol). These radicals can be divided into three groups: 1. The OH radicals which reduce the ferricytochrome c with a yield of (30 +/- 10)% and methemoglobin with a yield of (40 +/- 10)%. They do not reduce metmyoglobin. The reduction is not a normal bimolecular reaction but is most probably an intramolecular electron transfer of a protein radical. 2. Methanol and ethanol radicals which reduce all three hemoproteins with a yield of (100 +/- 5)%. This reduction is a normal bimolecular reaction. 3. Glycerol radicals which do not reduce the ferrihemoproteins under our experimental conditions. Ethylene glycol radicals do not reduce ferricytochrome c and metmyoglobin but they do reduce methemoglobin with a yield of (30 +/- 10)%.
mesh_terms: D000438:Alcohols; D003574:Cytochrome c Group; D000431:Ethanol; D005026:Ethylene Glycols; D005609:Free Radicals; D005720:Gamma Rays; D005990:Glycerol; D006420:Hemeproteins; D006900:Hydroxylation; D000432:Methanol; D008706:Methemoglobin; D008786:Metmyoglobin; D010084:Oxidation-Reduction
chemical_list: D000438:Alcohols; D003574:Cytochrome c Group; D005026:Ethylene Glycols; D005609:Free Radicals; D006420:Hemeproteins; D008786:Metmyoglobin; D000431:Ethanol; D008706:Methemoglobin; D005990:Glycerol; D000432:Methanol