Biogenesis of the cytochrome bc1 complex of yeast mitochondria. A precursor form of the cytoplasmically made subunit V.
The cytoplasmically made subunit V of the yeast mitochondrial cytochrome bc1 complex is synthesized as a larger polypeptide in vitro. This was shown by programming a reticulocyte lysate with yeast RNA and immunoprecipitating the labeled translation products with a subunit V-specific antiserum. The larger form of subunit V could also be detected in pulse-labeled spheroplasts; upon a subsequent chase, most of it disappeared. A proteolytic fingerprint of the larger form was closely similar to that of the mature subunit. These data suggest that the cytoplasmically made subunit V is translated as a larger precursor which is cleaved to the mature subunit either during or after its entry into the mitochondria.
D000818:Animals; D003580:Cytochromes / Q000096:biosynthesis*; D003575:Cytochromes c1 / Q000096:biosynthesis; D004792:Enzyme Precursors / Q000096:biosynthesis; D046911:Macromolecular Substances; D008928:Mitochondria / Q000201:enzymology*; D010446:Peptide Fragments / Q000032:analysis; D014176:Protein Biosynthesis; D012156:Reticulocytes / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
D003580:Cytochromes; D004792:Enzyme Precursors; D046911:Macromolecular Substances; D010446:Peptide Fragments; D003575:Cytochromes c1