Depression of bovine monocyte chemotactic responses by bovine viral diarrhea virus.
Incubation of bovine peripheral blood monocytes with bovine viral diarrhea virus (either Singer or NY-1 strain) caused a consistent, statistically significant decrease in their random locomotion (no chemoattractant) and chemotaxis towards a chemotactic lymphokine. Chemotaxis was determined by a modification of the Boyden method. Incubation of bovine viral diarrhea virus with mononuclear cells depressed chemotaxis by a mean of 56% (P less than 0.0005). Heat-killed virus had no effect on monocyte motility. Data suggest that bovine viral diarrhea virus can rapidly suppress monocyte functions in vitro, but by unknown mechanisms, not by killing cells.
D000818:Animals; D002417:Cattle; D002634:Chemotaxis, Leukocyte; D001908:Diarrhea Viruses, Bovine Viral / Q000276:immunology*; D005260:Female; D007111:Immunity, Cellular; D008297:Male; D009000:Monocytes / Q000276:immunology* / Q000382:microbiology; D012328:RNA Viruses / Q000276:immunology*