Characterization of human papillomavirus 3 in warts of a renal allograft patient.
Multiple flat wart-like lesions of a renal allograft recipient were shown to contain HPV 3 or a serologically crossreacting virus by indirect immunofluorescence with monospecific animal antisera against HPV [1--5]. The patient's serum revealed virus specific antibodies (immunofluorescence titer 1/80). Papillomaviruses were isolated and after in vitro iodination 3 major proteins (MW 70.000, 56.000 and 43.000) were detected by SDS polyacryalmide gel electrophoresis. DNA was extracted from the warts and cleaved with the restriction endonuclease Hae III. Distinct bands were discernible within the background of cellular DNA and these fragments were identified as papillomavirus DNA by blot hypbridization with 32P-labeled viral DNA.
D000328:Adult; D000956:Antigens, Viral; D004279:DNA, Viral; D005260:Female; D006801:Humans; D016030:Kidney Transplantation; D027383:Papillomaviridae / Q000276:immunology / Q000302:isolation & purification*; D012871:Skin Diseases / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D014184:Transplantation, Homologous; D014860:Warts / Q000276:immunology* / Q000382:microbiology / Q000473:pathology
D000956:Antigens, Viral; D004279:DNA, Viral