title: Thiobacillus acidophilus sp. nov.; isolation and some physiological characteristics.
abstract: After a brief exposition to glucose, Thiobacillus acidophilus was isolated from a culture of iron-grown T. ferrooxidans. Physicochemical analysis of its DNA showed a G+C content of 62.9-63.2%. The new isolate grows best at 25-30 degrees C and at pH 3.0. Growth is possible between pH 1.5 and 6.0. Thiobacillus acidophilus is apparently strictly aerobic. Ammonium salts are the only suitable source of nitrogen. The bacterium is a facultative autotroph. In addition to elemental sulfur, it obtains energy from organic compounds such as D-glucose, D-galactose, D-fructose, D-mannitol, D-xylose, D-ribose, D-arabinose, L-arabinose, sucrose, sodium citrate, malic acid,dl-aspartic acid, and dl-glutamic acid. Thiobacillus acidophilus possesses the key enzymes of the tricarboxylic acid (TCA) cycle including NAD-and NADP-linked isocitric dehydrogenase and alpha-ketoglutarate dehydrogenase, and the key enzymes of the hexose monophosphate pathway (glucose-6-phosphate and 6-phosphogluconate dehydrogenase, and fructose 1,6-diphosphate aldolase). NADH oxidase has been found in particulate fraction of extracts. Rhodanese and thiosulfate oxidase have also been detected.
mesh_terms: D000332:Aerobiosis; D000596:Amino Acids / Q000378:metabolism; D050260:Carbohydrate Metabolism; D002474:Cell-Free System; D002952:Citric Acid Cycle; D003470:Culture Media; D003596:Cytosine / Q000032:analysis; D004269:DNA, Bacterial / Q000032:analysis; D005947:Glucose / Q000378:metabolism; D006147:Guanine / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008858:Microscopy, Phase-Contrast; D010088:Oxidoreductases / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D013466:Sulfurtransferases / Q000378:metabolism; D013696:Temperature; D013855:Thiobacillus / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism; D014461:Ultracentrifugation
chemical_list: D000596:Amino Acids; D003470:Culture Media; D004269:DNA, Bacterial; D006147:Guanine; D003596:Cytosine; D010088:Oxidoreductases; D013466:Sulfurtransferases; D005947:Glucose