Expression of galactose-1-p-uridyltransferase in Chinese hamster x human galactosemia somatic cell hybrids.
Lymphocytes from a patient with classic galactosemia (GALT deficiency) were hybridized with a Chinese hamster cell line.  Electrophoretic valuation of GALT in 31 independently derived interspecific hybrid clones failed to demonstrate expression of the human GALT gene even when human chromosome 9 was present. Possible mechanisms for this lack of expression are presented.
D000818:Animals; D002460:Cell Line; D002874:Chromosome Mapping; D002906:Chromosomes, Human, 6-12 and X; D002999:Clone Cells; D006224:Cricetinae; D003412:Cricetulus; D005693:Galactosemias / Q000235:genetics*; D005796:Genes; D006801:Humans; D006822:Hybrid Cells; D008214:Lymphocytes; D009713:Nucleotidyltransferases / Q000235:genetics*; D005694:UTP-Hexose-1-Phosphate Uridylyltransferase / Q000235:genetics*
D009713:Nucleotidyltransferases; D005694:UTP-Hexose-1-Phosphate Uridylyltransferase