[Effect of chronic Phenoclor DP6 ingestion of some liver enzymatic activities in rats (author's transl)].
Rats were fed diet containing 100 ppm (wet weight) of Phenoclor DP6 for 4 weeks. The influence of DP6 was studied by determining the activity of hepatic microsomal enzymes namely : aniline hydroxylase, P-nitroanisole O-demethylase, aminopyrine N-demethylase, methylaniline N-demethylase. The cytochrome P450 and cytochrome b5 liver contents were measured. The activities of Na+ K+ and Mg++ATPases and of G6Phosphatase in the liver were also determined. In the liver of treated rats, the results show a large increased activity of microsomal drug metabolizing enzymes, cytochrome b5 and P450 contents and decreased ATPases and G6Pase specific activities. However total liver activities of ATPases and G6Pase were little modified by ingestion of Phenoclor DP6. The spectra given by cytochrome P450-DP6 binding was a typical type I.
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000633:Aminopyrine N-Demethylase / Q000378:metabolism; D000814:Aniline Compounds / Q000378:metabolism; D000818:Animals; D005952:Glucose-6-Phosphatase / Q000378:metabolism; D006900:Hydroxylation; D008658:Inactivation, Metabolic; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D009575:Nitroanisole O-Demethylase / Q000378:metabolism; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D051381:Rats
D000814:Aniline Compounds; D011078:Polychlorinated Biphenyls; D009575:Nitroanisole O-Demethylase; D000633:Aminopyrine N-Demethylase; D005952:Glucose-6-Phosphatase; D000251:Adenosine Triphosphatases
Intoxication alimentaire par les polychlorobiphenyles (PCB) chez le rat : V- Influence sur l'activité de quelques enzymes hépatiques.