Characteristics of infection of B and T lymphocytes from mice after inoculation with cytomegalovirus.
Viremia was produced by inoculating intravenously BALB/c mice with murine cytomegalovirus. Virus was detected in plasma and granulocytes only during the first 8 days after infection. Lymphocyte-associated viremia, detectable by cocultivation on syngeneic or allogeneic fibroblasts, persisted for at least 4 weeks. Eight to 10 days after infection, sonicated lymphocytes had no demonstrable free virus. When whole lymphocytes with no demonstrable free virus were enclosed in a Millipore chamber and placed on a fibroblastic feeder layer, T cells produced free virus but B cells did not. Compared to normal calf serum, specific hyperimmune serum reduced B cell-associated infectious centers by 74% and T cell-associated infectious centers by only 38%. Normal mouse sera reduced by 36% and 30% infectious center production by B cells and T cells, respectively. Lymphocytes enriched with Fc receptor-positive cells produced significantly more infectious centers than receptor-negative cells.
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D001402:B-Lymphocytes / Q000382:microbiology*; D003587:Cytomegalovirus / Q000254:growth & development / Q000276:immunology; D003586:Cytomegalovirus Infections / Q000097:blood* / Q000276:immunology; D003588:Cytopathogenic Effect, Viral; D005260:Female; D007106:Immune Sera; D007700:Kinetics; D008297:Male; D051379:Mice; D008934:Mitogens / Q000494:pharmacology; D013601:T-Lymphocytes / Q000382:microbiology*
D000914:Antibodies, Viral; D007106:Immune Sera; D008934:Mitogens