Heteroduplex analysis of the RNA of clone 3 Moloney murine sarcoma virus.
Heteroduplex analysis of the RNA isolated from purified virions of clone 3 Moloney murine sarcoma virus (M-MSV) hybridized to cDNA's from Moloney murine leukemia virus (M-MLV) and clone 124 M-MSV shows that the main physical component of clone 3 RNA is missing all or most of the 1.5-kilobase (kb) clone 124 M-MSV specific sequence denoted beta s (S. Hu et al. Cell 10:469--477, 1977). This sequence is either deleted in clone 3 RNA or substituted by a very short (0.3-kilobase) sequence. In other respects, clone 3 and clone 124 RNAs show the same heteroduplex structure relative to M-MLV. Since beta s is believed to contain the src gene(s) of clone 124 RNA, this result leaves as an unresolved question the nature of the src gene(s) of the clone 3 M-MSV RNA complex.
D001483:Base Sequence; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D005814:Genes, Viral; D008979:Moloney murine leukemia virus / Q000032:analysis*; D009692:Nucleic Acid Heteroduplexes / Q000032:analysis*; D012367:RNA, Viral / Q000032:analysis*; D009053:Sarcoma Viruses, Murine / Q000032:analysis* / Q000235:genetics
D009692:Nucleic Acid Heteroduplexes; D012367:RNA, Viral