title: Maximizing gene expression on a plasmid using recombination in vitro.
mesh_terms: D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D007763:Lac Operon; D009876:Operon; D010957:Plasmids; D014176:Protein Biosynthesis
chemical_list: D004262:DNA Restriction Enzymes