Intracellular forms of simian virus 40 nucleoprotein complexes. I. Methods of isolation and characterization in CV-1 cells.
A new method was developed for isolation of intracellular forms of simian virus 40 (SV40) nucleoprotein complexes from SV40-infected CV-1 cells late in the infectious cycle. In contrast to the Triton extraction method, which yields only a 60-70S complex, this new procedure yielded three forms of SV40 nucleoprotein complexes: complex I, complex II, and the nature virion (V). The three nucleoprotein complexes differed in physical as well as biochemical properties. Complex I, which is only a small portion of the total SV42 nucleoprotein complexes late during infection, was active in synthesizing both SV40-specific DNA and RNA. Pulse-labeling experiments suggest the following metabolic pathway: I leads to II leads to V. Conversion of complex I to II occurred shortly after the completion of SV40 DNA replication and resulted in the inactivation of the biosynthetic activities of I.
D000818:Animals; D002460:Cell Line; D004279:DNA, Viral / Q000032:analysis / Q000302:isolation & purification*; D000882:Haplorhini; D007668:Kidney; D008722:Methods; D013539:Simian virus 40 / Q000032:analysis*; D014764:Viral Proteins / Q000032:analysis / Q000302:isolation & purification*
D004279:DNA, Viral; D014764:Viral Proteins