title: Effects of pesticides on 3H-dihydrotestosterone binding to cytosol proteins from various tissues of the mouse.
abstract: These in vitro studies, utilizing cytosols prepared from various tissues (viz. anterior prostate gland, seminal vesicle, kidney and liver) taken from mice 3 days post-castration, were undertaken in order to determine whether or not certain pesticides could alter the binding of 3H-DHT to cytosolic androgen binding components. Estrone, cyproterone acetate, and unlabeled DHT were employed to confirm the presence of specific 3H-DHT binding components in the cytosols. The effects of various pesticides on 3H-DHT binding in these tissue cytosols were then assessed. Parathion (10(-8)--10(-5) M) was found to be an effective inhibitor of total 3H-DHT binding in the prostate, seminal veiscle, kidney and liver. This organophosphate was unable to compete with 3H-DHT for cytosol binding sites in the intestine. Similar in vitro binding studies using dieldrin, DDT, or carbaryl failed to reveal any interference with 3H-DHT binding in any of the tissue studied. The mechanism of parathion's interference with 3H-DHT binding is unclear.
mesh_terms: D000818:Animals; D012721:Carbaryl / Q000494:pharmacology; D003534:Cyproterone / Q000494:pharmacology; D003600:Cytosol / Q000378:metabolism*; D003634:DDT / Q000494:pharmacology; D004026:Dieldrin / Q000494:pharmacology; D013196:Dihydrotestosterone / Q000378:metabolism*; D004970:Estrone / Q000494:pharmacology; D066298:In Vitro Techniques; D007306:Insecticides / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D010278:Parathion / Q000494:pharmacology; D011467:Prostate / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000378:metabolism*; D012669:Seminal Vesicles / Q000378:metabolism
chemical_list: D007306:Insecticides; D011956:Receptors, Cell Surface; D013196:Dihydrotestosterone; D004970:Estrone; D010278:Parathion; D003634:DDT; D003534:Cyproterone; D004026:Dieldrin; D012721:Carbaryl