Experimental intraocular malignancy: the effect of intracameral perfusion.
Transplantable Brown-Pearce carcinoma was adapted successfully in the rabbit anterior chamber. Regression of tumor growth was attained on tri-weekly perfusion of the AC with 10 micromolar of methotrexate. Tumor cyclic nucleotide phosphodiesterase (PDE) and protein activator were found to be markedly depressed during the course of chemotherapy and the PDE cAMP/cGMP ratio was similarly altered. Corroborative light and electron-microscopic studies showed specific alterations of intracellular organelles in relation to MTX and tumor cell death. These findings suggest that metabolic pathways of cyclic nucleotides are important biochemical modulators of neoplastic cells. The method of intraocular perfusion precludes systemic toxic effects and avoids compromising the animals' immunocompetence.
D000818:Animals; D000867:Anterior Chamber / Q000601:surgery; D002284:Carcinoma, Brown-Pearce / Q000188:drug therapy* / Q000201:enzymology / Q000648:ultrastructure; D010478:Chemotherapy, Cancer, Regional Perfusion; D005134:Eye Neoplasms / Q000188:drug therapy* / Q000201:enzymology / Q000648:ultrastructure; D005260:Female; D008297:Male; D008727:Methotrexate / Q000627:therapeutic use*; D008854:Microscopy, Electron; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000188:drug therapy; D010727:Phosphoric Diester Hydrolases / Q000032:analysis; D011817:Rabbits; D013762:Tetrahydrofolate Dehydrogenase / Q000032:analysis
D013762:Tetrahydrofolate Dehydrogenase; D010727:Phosphoric Diester Hydrolases; D008727:Methotrexate