Conditions for hormone-stimulated expression of endogenous C57Bl strain-associated mammary tumor virus genome.
Full MMTV-Y gene expression can occur in dexamethasone-insulin-prolactin-stimulated cell cultured derived from C57Bl/10 mammary adenocarcinomas induced by syncarcinogenic action of chemical carcinogens (dimethylbenzanthracene), and mammotropic hormones (estrogen and prolactin). The rate of the hormone-stimulated virus production, as determined by biochemical, immunological and electron microscopical methods, was comparable to the level of spontaneous MMTV production by cells of established mouse mammary cancer cultures (CCL-51 and Mm5mt/cl). On the contrary, no virus production has been detected in hormone-stimulated cultures derived from C57Bl/10 mammary tumors induced by chemical carcinogen alone (urethan).
D000230:Adenocarcinoma / Q000209:etiology; D000818:Animals; D002478:Cells, Cultured; D003907:Dexamethasone / Q000494:pharmacology*; D005260:Female; D005814:Genes, Viral; D007328:Insulin / Q000494:pharmacology*; D008325:Mammary Neoplasms, Experimental / Q000139:chemically induced / Q000209:etiology* / Q000382:microbiology; D008324:Mammary Tumor Virus, Mouse / Q000235:genetics* / Q000302:isolation & purification; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D009376:Neoplasms, Hormone-Dependent / Q000209:etiology / Q000382:microbiology; D011388:Prolactin / Q000494:pharmacology*
D007328:Insulin; D003907:Dexamethasone; D011388:Prolactin