Antibody-dependent autologous lymphocyte cytotoxicity against cells freshly transformed by Epstein-Barr virus.
Cytotoxicity against lymphocytes freshly transformed by EBV was studied in an autologous system by trypan blue exclusion of EBNA-positive cells and 51Cr-release assay. When EBV-tranformed lymphocytes were incubated with EBV-positive serum and autologous unfractionated lymphocytes or K-cells from sero-positive healthy donors, the transformed cells were significantly damaged. The cytotoxicity against newly produced EBNA-positive lymphocytes was also remarkable when sero-positive healthy donor lymphocytes were exposed to EBV, immediately followed by incubation with positive serum. In contrast, EBV-transformed cells incubated with autologous lymphocytes or T-cells alone were not significantly affected. EBV-positive serum alone was also not cytotoxic, regardless of inactivation. These results strongly suggest that antibody-dependent cellular cytotoxicity (ADCC) plays an important role in immunological defence against EBV oncogenesis in healthy humans. However, ADCC was not seen when immunosuppressed patients were examined.
D000914:Antibodies, Viral / Q000008:administration & dosage; D000920:Antibody-Dependent Cell Cytotoxicity; D000956:Antigens, Viral; D002467:Cell Nucleus / Q000276:immunology; D002471:Cell Transformation, Neoplastic; D002478:Cells, Cultured; D004854:Herpesvirus 4, Human / Q000276:immunology; D006801:Humans; D007165:Immunosuppression Therapy; D066298:In Vitro Techniques; D007694:Killer Cells, Natural / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral