Concanavalin A inhibits tissue factor coagulant activity.
Concanavalin A (con A) is a potent inhibitor of coagulant activity of native tissue factor. Coagulant activity is recovered by addition of alpha-methyl-D-glucoside to inhibited tissue factor. Inclusion of alpha-methyl-D-glucose during incubation of con A with tissue factor preserves coagulant activity. These data suggest that con A interacts reversibly with a carbohydrate residue in such a way as to inhibit coagulant activity of the molecule. Purified tissue factor apoprotein has been recombined with mixed brain phospholipids or purified phospholipids (phosphatidyl ethanolamine or a mixture of phosphatidyl choline with phosphatidyl serine). These preparations were also completely but reversibly inhibited by con A. Thus, purified tissue factor apoprotein appears to donate the affected carbohydrate residue.
D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects*; D002417:Cattle; D002850:Chromatography, Gel; D003208:Concanavalin A / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D003864:Depression, Chemical; D005167:Factor VII; D005170:Factor X; D008074:Lipoproteins / Q000037:antagonists & inhibitors* / Q000302:isolation & purification; D008757:Methylglucosides / Q000494:pharmacology; D010713:Phosphatidylcholines / Q000494:pharmacology; D010714:Phosphatidylethanolamines / Q000494:pharmacology; D010718:Phosphatidylserines / Q000494:pharmacology; D013997:Time Factors; D014020:Tissue Extracts
D008074:Lipoproteins; D008757:Methylglucosides; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010718:Phosphatidylserines; D014020:Tissue Extracts; D003208:Concanavalin A; D005167:Factor VII; D005170:Factor X