The specific, diverse effects of purine and pyrimidine nucleoside-5'-di(tri)-3'-diphosphates on the eucaryote translation system in vitro.
All the eight 5'-di(tri)-3'-diphosphates of four common ribonucleosides were prepared by the enzymic pyrophosphoryl transfer catalysed by Streptomyces adephospholyticus ATP:nucleotide pyrophosphokinase (E.C.2.7.6.4) form dATP to the respective 5'-phosphates, and their effects on the translation of mRNAs by a wheat germ system in vitro were studied. (p) ppPupp decreased the total 14C-leucine incorporation directed by a rat liver mRNA whereas (p) ppPypp did not. With a silkworm pupa ovary mRNA, distinctly reverase results were obtained. Gel electrophoretic profiles of the translation products disclosed the mRNA species-specific stimulatory or inhibitory effects for each of the polyphosphates tested.
D000227:Adenine Nucleotides / Q000494:pharmacology*; D000818:Animals; D003597:Cytosine Nucleotides / Q000494:pharmacology*; D017854:Diphosphotransferases; D006150:Guanine Nucleotides / Q000494:pharmacology*; D006159:Guanosine Tetraphosphate / Q000494:pharmacology*; D007700:Kinetics; D008099:Liver / Q000378:metabolism; D009711:Nucleotides / Q000378:metabolism; D010770:Phosphotransferases / Q000378:metabolism*; D010944:Plants / Q000378:metabolism; D014176:Protein Biosynthesis / Q000187:drug effects*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D013302:Streptomyces / Q000201:enzymology; D014908:Triticum / Q000378:metabolism; D014500:Uracil Nucleotides / Q000494:pharmacology*
D000227:Adenine Nucleotides; D003597:Cytosine Nucleotides; D006150:Guanine Nucleotides; D009711:Nucleotides; D012333:RNA, Messenger; D014500:Uracil Nucleotides; C017990:adenosine 3'-diphosphate 5'-diphosphate; D006159:Guanosine Tetraphosphate; C025329:uridine 3'-diphosphate 5'-diphosphate; C025328:cytidine 3'-diphosphate 5'-diphosphate; D010770:Phosphotransferases; D017854:Diphosphotransferases; C021900:nucleotide pyrophosphokinase