A thermally induced alteration in lysosome membranes: salt permeability at 0 and 37 degrees C.
Preparations of radioactive lysosomes were obtained from mouse kidney after injection of radioactive iodine-labeled bovine ribonuclease. Stability of these lysosomes in various media was estimated from measurements of proteolytic activity towards the ribonuclease, and of ribonuclease retention in particles. The lysosomes were stable at 37 degrees C in isotonic, sucrose-free solutions of KCl, NaCl, and potassium acetate, and in mixtures of these with MgCl2, showing that these salts are relatively impermeant through the lysosomal membranes. The membranes were less permeable to Na+ than to K+. Both KCl and NaCl exerted their optimal protective effects over a broad concentration range above 0.125 M in 0.025 M acetate buffer. Mg2+ enhanced the protective effect of both K4 and Na+; the osmotic effect of 0.075 M NaC1-0.05 M MgCl2 was indistinguishable during the entire course of ribonuclease digestion from that of isotonic sucrose. Osmotic protection by KC1-MgC12 was demonstrated over the H range5.5-7.0. A marked alteration in membrane properties occurs at lower temperatures in 0.11 M KC1-0.01 M MgCl2 such that, at 0 degrees C, K+ permeability is much higher than at 37 degrees C, as shown by a several-fold decrease in stability at the lower temperature.
D000818:Animals; D002712:Chlorides / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D007668:Kidney / Q000378:metabolism; D008247:Lysosomes / Q000187:drug effects / Q000378:metabolism*; D008274:Magnesium / Q000494:pharmacology*; D008433:Mathematics; D008566:Membranes / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D009994:Osmolar Concentration; D010539:Permeability; D011189:Potassium Chloride / Q000494:pharmacology*; D012965:Sodium Chloride / Q000494:pharmacology*; D013395:Sucrose / Q000494:pharmacology; D013696:Temperature
D002712:Chlorides; D012965:Sodium Chloride; D013395:Sucrose; D011189:Potassium Chloride; D008274:Magnesium