Extraction of soluble antigens of Epstein-Barr virus, Herpesvirus salmirl, and Herpesvirus ateles with the use of glycine.
For extraction of soluble antigen from cells infected with Epstein-Barr virus, Herpesvirus salmirl, and H. ateles, 0.1 M glycine (pH 9.5) was used. This method yielded increased amounts of the antigen containing much less cell debris. Lymphoblastoid cells infected with Epstein-Barr virus could maintain up to 50% viability after the extraction procedure. These cells could be used again after an appropriate interval in culture. The usefulness of this technique is discussed.
D000956:Antigens, Viral / Q000032:analysis*; D005998:Glycine; D006564:Herpesviridae / Q000276:immunology*; D006567:Herpesvirus 2, Saimiriine / Q000276:immunology*; D004854:Herpesvirus 4, Human / Q000276:immunology*; D007158:Immunologic Techniques; D008828:Microbiological Techniques
D000956:Antigens, Viral; D005998:Glycine