Defective retrovirus-like 30S RNA species of rat and mouse cells are infectious if packaged by type C helper virus.
RNA species with properties of defective retrovirus-like 30S RNA genomes have previously been detected in both rats and mice and in some rat and mouse retroviruses. Using cell lines which express high levels of this retrovirus-like RNA, we formed pseudotypes of the 30S RNAs with helper-independent type C viruses. A pseudotype virus complex containing a mouse 30S subunit was transmitted to rat cells, and a pseudotype virus complex containing a rat 30S subunit was transmitted to bat cells. In other transmission experiments, a rat 30S subunit was isolated in nonproducer bat cells without detectable expression of the helper-independent type C virus used to pseudotype it. The results provide further support for the retrovirus-like nature of the rat 30S subunit and provide evidence which supports the protovirus hypothesis proposed by Temin.
D000818:Animals; D002460:Cell Line; D002685:Chiroptera; D006378:Helper Viruses / Q000235:genetics / Q000254:growth & development*; D006824:Hybridization, Genetic; D051379:Mice; D008979:Moloney murine leukemia virus / Q000235:genetics / Q000254:growth & development*; D012367:RNA, Viral / Q000235:genetics; D051381:Rats; D012190:Retroviridae / Q000235:genetics / Q000254:growth & development*; D014779:Virus Replication
D012367:RNA, Viral