Effects of swab materials and transport media on Aujeszky's disease virus.
The effect of swab materials on the recovery of Aujeszky's disease virus from various transport media held at 4 degrees C was investigated over a five day period. No significant loss in infectivity was found in control preparations, approximately 50 per cent of infectivity was recovered from fluids containing applicator wire and approximately 10 per cent from fluids containing polyester fibre or cotton wool. Virus recovery from fluids containing wooden applicator sticks ranged from no virus recovery in protein free media to from 1 to 10 per cent in protein containing media. Freezing followed by thawing was the most effective of the physical methods used for eluting virus from swab materials.
D046508:Culture Techniques; D006564:Herpesviridae / Q000302:isolation & purification*; D011558:Herpesvirus 1, Suid / Q000302:isolation & purification*; D008828:Microbiological Techniques