In vivo transcription and translation of R-plasmid 538-1 DNA in Escherichia coli.
In vivo transcription and translation of R-plasmid 538-1 in E. coli was analyzed. Transcription of individual restriction fragments was determined qualitatively by utilizing the techniques developed by Southern (27), and quantitatively by carrying out DNA-RNA filter hybridization. The most active region of R-plasmid transcription in strains repressed for conjugal transfer was found to occur in the region of the R-plasmid carrying the antibiotic resistance genes. In plasmids derepressed for conjugal transfer, a high level of transcription from the transfer gene region was also observed. When strains carrying R538-1drd were induced with Hg++ a high level of transcription was observed from the region of the R-plasmid carrying the genes for Hgr. Hybrid ColE1 plasmids carrying restriction fragments from the antibiotic resistance region of R538-1 were segregated into minicells. Labeling of the minicells with 35S-methionine allowed identification of the proteins coded by the fragments. A limited number of proteins were detected, and several of these have been correlated with the antibiotic resistance genes carried by R538-1.
D001426:Bacterial Proteins / Q000235:genetics; D002701:Chloramphenicol / Q000494:pharmacology; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D008628:Mercury / Q000494:pharmacology; D014176:Protein Biosynthesis; D011815:R Factors; D013307:Streptomycin / Q000494:pharmacology; D013411:Sulfadiazine / Q000494:pharmacology; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D013411:Sulfadiazine; D002701:Chloramphenicol; D004262:DNA Restriction Enzymes; D008628:Mercury; D013307:Streptomycin