Appearance of hCG-receptor after conversion of newborn ovarian cells into testicular structures by H-Y antigen in vitro.
In a previous report (Zenzes et al., 1978b) it was shown that dissociated ovarian cells of newborn rats in vitro, if exposed to H-Y antigen, reorganize into testicular structures. The current study was designed to see whether this morphological conversion also results in a functional conversion. The LH/hCG receptor was used as a parameter characteristic for the newborn testis, but not for the newborn ovary. In the converted ovary, the LH/hCG receptor becomes detectable a few hours after onset of the culture and remains continuously present afterward. The appearance of this receptor may be due to a hormone-like action of H-Y antigen.
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000378:metabolism*; D003513:Cycloheximide / Q000494:pharmacology; D005260:Female; D006182:H-Y Antigen; D008297:Male; D010053:Ovary / Q000166:cytology*; D051381:Rats; D011956:Receptors, Cell Surface / Q000378:metabolism*; D013737:Testis / Q000378:metabolism*
D006063:Chorionic Gonadotropin; D006182:H-Y Antigen; D011956:Receptors, Cell Surface; D003513:Cycloheximide