Adenosine-adenosine deaminase modulation of histamine release from human basophils in vitro.
Since extracellular adenosine is a physiologically important regulator of adenylate cyclase and cell function in various mammalian tissues, we have examined the effect of adenosine on histamine release from human basophils. Adenosine inhibited IgE-mediated histamine release by its ability to increase leukocyte cyclic AMP levels; the same concentrations of adenosine which inhibited histamine release increased the cyclic AMP level of mixed leukocytes. Inhibition of histamine release was also observed with an adenosine deaminase (ADA) inhibitor [erythro-9-(2-hydroxy-3-nonyl)-adenine: EHNA] in the presence of autologous serum. We suggest that the adenosine-ADA system normally modulates histamine release and that this contributes to the severe combined immune deficiency (SCID) associated with a lack of ADA.
D000241:Adenosine / Q000378:metabolism* / Q000494:pharmacology; D000243:Adenosine Deaminase / Q000378:metabolism*; D000941:Antigens; D001491:Basophils / Q000378:metabolism*; D000242:Cyclic AMP / Q000378:metabolism; D004306:Dose-Response Relationship, Immunologic; D006636:Histamine Release; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D009700:Nucleoside Deaminases / Q000378:metabolism*
D000941:Antigens; D007073:Immunoglobulin E; D000242:Cyclic AMP; D009700:Nucleoside Deaminases; D000243:Adenosine Deaminase; D000241:Adenosine