Type C virus induced by iododeoxyuridine in the human embryonic cell strain HEL-12.
The induction of a type C virus from a strain of human embryonic lung cells (HEL-12) by iododeoxyuridine (IdUrd) was examined at various times during in vitro propagation. IdUrd did not elicit type C virus production immediately following initiation of cultures from frozen primary HEL-12 cells. After overnight treatment with 30 microgram/ml IdUrd the cells expressed viral antigens and produced a type  C virus between the 25th and 80th day of in vitro growth. Production of the induced type C virus was transient. Single-cell clones of the parental HEL-12 strain were likewise susceptible to type C virus production by IdUrd. The ability of IdUrd to induce virus terminated with the onset of spontaneous type C virus production from HEL-12 cells at between 80 and 120 days of in vitro growth.
D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D002999:Clone Cells; D006801:Humans; D007065:Idoxuridine / Q000494:pharmacology*; D008168:Lung / Q000196:embryology; D012190:Retroviridae / Q000187:drug effects* / Q000254:growth & development / Q000276:immunology; D014779:Virus Replication / Q000187:drug effects*
D000956:Antigens, Viral; D007065:Idoxuridine