Procedures used in the induction of mitotic recombination and mutation in the yeast Saccharomyces cerevisiae.
Techniques are described for the use of various yeast strains to detect the induction of (1) mitotic crossing-over, (2) mitotic gene conversion, (3) forward mutation and (4) reverse mutation. The technique for the detection of mitotic crossing over is based on a diploid that carries two different alleles of the gene locus ade2. These alleles differ in their extent of colony pigmentation engendered on low-adenine media, and they complement each other to the effect that the diploid is white. Mitotic crossing over results in the formation of twin-sectored colonies with a red and a pink sector. The technique for the detection of mitotic gene conversion is based on the use of a heteroallelic diploid carrying two non-complementing alleles that cause a nutritional requirement. Mitotic gene conversion leads to the restoration of intact and dominant wild-type alleles that alleviate the nutritional requirement so that convertant cells can be selected on a minimal medium. The forward mutation technique is based on the use of a haploid strain with a defect in the ade2-gene locus which causes the formation of red colonies. Induction of forward mutation in a number of other loci prevents the accumulation of this red pigment so that induction of mutation can be detected by the formation of pink and white colonies. The reverse mutation technique is based on the restoration or compensation of a mutational defect causing a growth requirement. Mutants can be selected for on a minimal medium.
D000225:Adenine / Q000378:metabolism; D000483:Alleles; D003470:Culture Media; D005796:Genes; D005821:Genetic Techniques; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008938:Mitosis; D009153:Mutagens / Q000494:pharmacology; D009154:Mutation; D010858:Pigmentation; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000254:growth & development
D003470:Culture Media; D007202:Indicators and Reagents; D009153:Mutagens; D000225:Adenine