Influence of 4-aminopyrazolopyrimidine on morphology, synthesis of triglyceride and protein and their secretion in rat hepatocytes.
The influence of 4-aminopyrazolopyrimidine (4-APP) on morphology and on synthesis and secretion ability of isolated rat hepatocytes was investigated: 4-APP was found to inhibit both the synthesis and secretion of proteins. The synthesis of triglycerides was unaffected by 4-APP, while the secretion of triglycerides was markedly reduced. Transmission electron microscopy revealed that 4-APP induced morphological changes in the smooth membrane systems, the smooth endoplasmic reticulum and the Golgi apparatus. The possibility that 4-APP inhibits lipoprotein formation by inhibition of the apoprotein synthesis is discussed.
D000818:Animals; D004721:Endoplasmic Reticulum / Q000648:ultrastructure; D005234:Fatty Liver / Q000139:chemically induced; D006056:Golgi Apparatus / Q000648:ultrastructure; D008079:Lipoproteins, VLDL / Q000378:metabolism; D008099:Liver / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008930:Mitochondria, Liver / Q000648:ultrastructure; D014176:Protein Biosynthesis; D011743:Pyrimidines / Q000633:toxicity*; D051381:Rats; D014280:Triglycerides / Q000096:biosynthesis*
D008079:Lipoproteins, VLDL; D011743:Pyrimidines; D014280:Triglycerides