Increased cyclic adenosine 3':5'-monophosphate phosphodiesterase activity in the epidermis of phorbol ester-treated mouse skin and in papillomas.
The potent tumor promoter, 12-O-tetradecanoylphorbol-13-acetate, produced a 2- to 3-fold increase in the activity of both the low- and high-affinity forms of cyclic adenosine 3':5'-monophosphate phosphodiesterase activity 13 hr after application to mouse skin. The magnitude of the enzyme induction correlated with the tumor-promoting activity of several doses of 12-O-tetradecanoylphorbol-13-acetate and of other phorbol esters. The induction of the low-affinity phosphodiesterase could be blocked by prior i.p. injection of the microtubule poisons, colchicine and vinblastine. The low-affinity cyclic adenosine 3':5'-monophosphate phosphodiesterase activity of the epidermal component of mouse skin papillomas produced by two-stage tumorigenesis was 3 times that of the surrounding uninvolved epidermis.
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000096:biosynthesis*; D000818:Animals; D003078:Colchicine / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects; D005260:Female; D051379:Mice; D009374:Neoplasms, Experimental / Q000201:enzymology; D010212:Papilloma / Q000201:enzymology*; D010703:Phorbol Esters / Q000494:pharmacology*; D010704:Phorbols / Q000494:pharmacology*; D012867:Skin / Q000187:drug effects* / Q000201:enzymology; D012878:Skin Neoplasms / Q000201:enzymology*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014747:Vinblastine / Q000494:pharmacology
D010703:Phorbol Esters; D010704:Phorbols; D014747:Vinblastine; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D013755:Tetradecanoylphorbol Acetate; D003078:Colchicine