Nucleotide sequence analysis of two simian virus 40 mutants with deletions in the late region of the genome.
Two mutants of simian virus 40, dl-1261 and dl-1262, have deletions that map between coordinated 0.90 and 0.95 (Cole et al., J. Virol 24:277--294, 1977). Both affect the structure of the two minor proteins VP2 and VP3. The precise location and size of the deletions have now been determined by nucleotide sequence analysis. Mutant dl-1261 is deleted of 54 base pairs, is temperature sensitive for the protein defined by the D complementation group, and promotes the synthesis of shorter VP2 and VP3 polypeptides. Mutant dl-1262 is viable irrespective of temperature and has a deletion of 36 base pairs, 23 of which overlap the deletion in dl-1261. Since these mutants produce normal VP1, the deleted regions probably have no function in the splicing of precursor RNA to the VP1 mRNA.
D001483:Base Sequence; D004279:DNA, Viral / Q000032:analysis*; D005814:Genes, Viral; D009154:Mutation; D009711:Nucleotides / Q000032:analysis*; D010455:Peptides / Q000235:genetics; D013539:Simian virus 40 / Q000032:analysis* / Q000235:genetics; D013696:Temperature; D014764:Viral Proteins / Q000235:genetics
D004279:DNA, Viral; D009711:Nucleotides; D010455:Peptides; D014764:Viral Proteins