BLV-LB virus-specific sequences in cattle with spontaneous and experimentally induced leukemia.
Poly(A)-containing RNAs were extracted directly from the culture fluid of BLV-producing continuous and short-term lymphocyte cultures. The abovesaid poly(A)-RNAs were used as a template to synthesize complementary [3H]DNA (BLV cDNA). BLV cDNA possessed a high degree of homology to poly(A)-RNA isolated directly from the blood plasma of a leukemic animal. In contrast of leukemic cattle BLV-related sequences in white blood cells of normal animals were absent. Leukemic cells of cattle with both, spontaneous and experimentally induced leukemia contained bovine leukemia virus sequences in their genomes.
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000382:microbiology*; D002478:Cells, Cultured; D003176:Complement C3 / Q000096:biosynthesis; D004279:DNA, Viral / Q000096:biosynthesis; D005814:Genes, Viral; D066298:In Vitro Techniques; D007938:Leukemia / Q000662:veterinary*; D001909:Leukemia Virus, Bovine; D008214:Lymphocytes / Q000382:microbiology; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000302:isolation & purification; D012367:RNA, Viral / Q000302:isolation & purification
D003176:Complement C3; D004279:DNA, Viral; D012367:RNA, Viral; D011061:Poly A