A rapid method for the determination of deoxycytidylate deaminase activity in pregnancy serum.
A method is described for determining the activity of deoxycytidylate deaminase in serum. The ammonia liberated from deoxycytidine monophosphate has been specifically determined by enzymatic amination of alpha-ketoglutarate using glutamate dehydrogenase. The concurrent oxidation of NADH2 at 340 nm was proportional to the ammonia liberated from deoxycytidine monophosphate. Using the technique described, a result would be available to the clinician in under 4.5 h. The "normal activity" for deoxycytidine deaminase in normal male, female and pregnancy sera has been determined.
D003844:DCMP Deaminase / Q000097:blood*; D005069:Evaluation Studies as Topic; D005260:Female; D005312:Fetal Blood / Q000201:enzymology; D005969:Glutamate Dehydrogenase / Q000097:blood; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008297:Male; D009709:Nucleotide Deaminases / Q000097:blood*; D011247:Pregnancy; D011262:Pregnancy Trimester, Second; D011263:Pregnancy Trimester, Third; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013997:Time Factors
D005969:Glutamate Dehydrogenase; D009709:Nucleotide Deaminases; D003844:DCMP Deaminase