Microanalytical RPC-5 columns for rapid chromatography of aminoacyl-tRNAs.
A microanalytical reversed-phase chromatographic system for the separation of aminoacyl-tRNA's on microbore (0.3cm X 25cm) columns is described. Chromatography of lysyl-tRNA from polyoma virus transformed mouse fibroblasts revealed at least seven identifiable isoacceptors. The system has the advantages of speed, sensitivity and excellent resolution of isoaccepting tRNA's.
D000818:Animals; D002845:Chromatography / Q000379:methods; D005347:Fibroblasts / Q000032:analysis*; D008832:Microchemistry / Q000379:methods; D011120:Polyomavirus / Q000032:analysis*; D012346:RNA, Transfer, Amino Acyl / Q000032:analysis*
D012346:RNA, Transfer, Amino Acyl