Simple method for preparation of specific antisera against viral proteins: rabbit antisera against equine infectious anemia virus proteins p26 and p16.
A simple method for preparation of highly specific antisera against equine infectious anemia virus proteins p26 and p16 is described. Viral proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The motility of the viral proteins in the gel was compared with standards. Unstained portions of the slab gel were sliced into 5-mm bands, emulsified with Freund's complete adjuvant, and injected into rabbits to produce specific antisera.
D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D007106:Immune Sera; D005779:Immunodiffusion; D004860:Infectious Anemia Virus, Equine / Q000032:analysis / Q000276:immunology*; D011817:Rabbits / Q000276:immunology*; D014764:Viral Proteins / Q000276:immunology* / Q000302:isolation & purification
D007106:Immune Sera; D014764:Viral Proteins