[DNA-protein interactions. Destabilizing activity of sheep pancreatic RNAase].
Evidence is presented that ovine pancreatic ribonuclease, a protein strictly homologous to bovine RNAase A but with one positive charge less, has a definite 'destabilizing' activity (quite similar to that of the bovine enzyme) on double-stranded DNA. This action of sheep pancreas RNAase has been measured by differential spectrophotometry and determining the thermal-transition profiles of the protein-DNA complexes.
D000818:Animals; D002417:Cattle; D004247:DNA / Q000378:metabolism*; D004720:Endonucleases / Q000494:pharmacology*; D009691:Nucleic Acid Denaturation; D010179:Pancreas / Q000201:enzymology; D012259:Ribonuclease, Pancreatic; D012260:Ribonucleases / Q000494:pharmacology*; D012756:Sheep; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature
D004247:DNA; D004720:Endonucleases; D012260:Ribonucleases; D012259:Ribonuclease, Pancreatic
Ricerche sulle interazioni DNA-proteine. Azione "destabilizzante" della RNAasi pancreatica ovina.