Departure from ideality in saturation binding assays.
In this paper we show that the measurement of cyclic AMP by a protein saturation assay deviates from ideality due to the presence of two classes of binding sites. An equation was derived which completely describes saturation assays and the effects on these of varying each of the component parameters was measured. The relevance of these findings to other protein saturation assays is discussed.
D001665:Binding Sites; D000242:Cyclic AMP / Q000032:analysis*; D008433:Mathematics; D011485:Protein Binding; D011869:Radioligand Assay / Q000379:methods*
D000242:Cyclic AMP