Small-scale techniques for the analysis of recombinant plasmids.
Using the cloning of part of the T-DNA of pTiC58 from Agrobacterium tumefaciens as an example, techniques are described which enable recombinant plasmids to be mapped and used as hybridization probes. In all cases the starting material is a colony of cells grown on an agar plate which is then subjected to lysis by lysozyme and Triton X-100 in volumes of the order of 300 microliters thus eliminating the need for handling and centrifuging liquid cultures under restrictive containment conditions.
D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000032:analysis*; D015252:Deoxyribonucleases, Type II Site-Specific; D008722:Methods; D010957:Plasmids; D012231:Rhizobium
D004274:DNA, Recombinant; D004262:DNA Restriction Enzymes; C025651:CCCGGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific