Effects of dibutyryl cyclic AMP and cytochalasin B on cultured human glioma cells.
Cultured human glioma cells (138 MG) exposed to dibutyryl cyclic AMP (dbc-AMP; 0.1--5 mM) attained an arborized shape with thin processes extending from a rounded cell body. Cytochalasin B (CB; 1--1 muM) induced similar morphological changes. The processes in both dbc-AMP and CB treated cells were formed by retraction of the cell margin. Colchicine (1muM) completely and liver treated phalloidin (0.1 mg/ml) partially inhibited the morphological alterations induced by dbc-AMP and CB. Dbc-AMP was found to arrest cell movement, cell division and uptake of 2-deoxy-D-glucose. CB has the same effects but was more potent. The effects of dbc-AMP and CB could be due to interference with a common cellular structure, e.g. microfilaments.
D003994:Bucladesine / Q000627:therapeutic use*; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D003571:Cytochalasin B / Q000627:therapeutic use*; D003599:Cytoskeleton / Q000187:drug effects; D003847:Deoxyglucose / Q000378:metabolism; D004353:Drug Evaluation, Preclinical; D005910:Glioma / Q000188:drug therapy* / Q000378:metabolism / Q000648:ultrastructure; D006801:Humans; D066298:In Vitro Techniques; D008855:Microscopy, Electron, Scanning; D008870:Microtubules / Q000187:drug effects
D003571:Cytochalasin B; D003994:Bucladesine; D003847:Deoxyglucose