Liver glutathione and glutathione reductase response of endotoxin-treated mice.
The decrease in the level of liver glutathione (GSH) in endotoxin-treated mice was in part due to formation of glutathione disulfide (GSSG). An electron-generating system (EGS) had no effect when incubated with soluble liver extracts from normal controls but resulted in recovery of GSH amounting to 25% in endotoxin-treated animals. Incubation in the absence of the EGS caused a decline of 16% in the GSH in extracts from normal animals compared with a 50% decrease in endotoxin-treated animals. Exclusion of nicotinamide adenine dinucleotide phosphate (NADP) from the EGS resulted in a slight decline in the GSH of the extract from the normal controls but 25% for the endotoxin-treated animals. Reduction of exogenous GSSG by the liver extracts required that exogenous NADP be added to ghe incubation mixtures.
D000818:Animals; D004220:Disulfides / Q000096:biosynthesis; D004731:Endotoxins / Q000494:pharmacology*; D005954:Glucosephosphate Dehydrogenase; D005978:Glutathione / Q000378:metabolism*; D005980:Glutathione Reductase / Q000378:metabolism*; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D051379:Mice; D009249:NADP; D009536:Niacinamide; D011506:Proteins / Q000032:analysis; D012477:Salmonella enteritidis / Q000276:immunology; D013053:Spectrophotometry; D014020:Tissue Extracts
D004220:Disulfides; D004731:Endotoxins; D011506:Proteins; D014020:Tissue Extracts; D009536:Niacinamide; D009249:NADP; D005954:Glucosephosphate Dehydrogenase; D005980:Glutathione Reductase; D005978:Glutathione