Effect of heat treatment on the performance of tryptose-sulfite-cycloserine agar for enumeration of Clostridium perfringens.
Dissolving dehydrated tryptose-sulfite-cycloserine agar by only boiling or microwaving was found to inhibit Clostridium perfringens colony development in pour plates when compared with C. perfringens recovery in tryptose-sulfite-cycloserine agar prepared by autoclaving.
D000362:Agar; D003016:Clostridium perfringens / Q000302:isolation & purification*; D003523:Cycloserine; D006358:Hot Temperature; D008872:Microwaves; D013242:Sterilization; D013447:Sulfites
D013447:Sulfites; D000362:Agar; D003523:Cycloserine