Active-site-specific reconstituted cobalt(II) horse-liver alcohol dehydrogenase. Changes of the spectra of the substrate trans-4-(N,N-dimethylamino)-cinnamaldehyde and of the catalytic cobalt ion upon ternary complex formation with NADH and 1,4,5,6-tetrahydronicotinamide--adenine dinucleotide.
D000429:Alcohol Oxidoreductases / Q000378:metabolism; D000447:Aldehydes; D000818:Animals; D001665:Binding Sites; D002934:Cinnamates; D003035:Cobalt; D006736:Horses; D008099:Liver / Q000201:enzymology*; D009243:NAD / Q000031:analogs & derivatives*; D011485:Protein Binding; D013053:Spectrophotometry; D015032:Zinc
D000447:Aldehydes; D002934:Cinnamates; D009243:NAD; D003035:Cobalt; D000429:Alcohol Oxidoreductases; D015032:Zinc