Spectrodensitometric determination of trichothecene mycotoxins with 4-(p-nitrobenzyl)pyridine on silica gel thin-layer chromatograms.
A simple method for the detection and spectrodensitometric determination of a number of trichothecene mycotoxins on silica gel layers based on a colour reaction between 4-(p-nitrobenzyl)pyridine and the 12,13-epoxy group in the trichothecene nucleus is described. The detection limits for the twelve trichothecenes examined were 0.025--0.2 micrograms per spot. Further, six of the twelve trichothecenes could be determined spectrodensitometrically in the range from ca. 0.05--0.2 to 10 micrograms per spot with a coefficient of variation of ca. 5%.
D002855:Chromatography, Thin Layer; D003720:Densitometry; D005506:Food Contamination / Q000032:analysis; D005782:Gels; D008832:Microchemistry; D009578:Nitrobenzenes; D012275:Oryza / Q000032:analysis; D011725:Pyridines; D012717:Sesquiterpenes / Q000032:analysis*; D012822:Silicon Dioxide; D013053:Spectrophotometry / Q000379:methods; D014255:Trichothecenes / Q000032:analysis*
D005782:Gels; D009578:Nitrobenzenes; D011725:Pyridines; D012717:Sesquiterpenes; D014255:Trichothecenes; C008633:4-(4-nitrobenzyl)pyridine; D012822:Silicon Dioxide