[Study of the cell walls of yeasts Rhodotorula. VI'nfluence of 2-hydroxybiphenyl on phosphatase activities of Rh. rubra (author's transl)].
The inhibition of acid phosphatase activity observed after culture of Rh. rubra in phosphate rich mediums is raised by the culture of this yeast in presence of 2-hydroxybiphenyl (OHph2). The cell wall alkaline phosphatase activity was inhibited by this derivative; When cultivated with OHph2 an intra and a more extracellular acid phosphatase activity appeared. The comparative studies of the two extracellular acid phosphatases secreted in the medium with or without the OHph2 show they have similar characteristics. They are eluated at the same time from Sephadex  G-200, DEAE- and CM-cellulose columns, and have the same Km. They are both glycoproteins, with the sugars forming the polyose fragment identical, but the enzyme secreted in the medium containing the OHph2 contains less sugar than the one secreted in the medium without OHph2. The appearance of this acid phosphatase activity was attributed to the alteration of the membrane glycosylating systems or to the important ultra structure modifications of the cell wall of Rh. rubra when this yeast is cultivated with OHph2.
D000135:Acid Phosphatase / Q000302:isolation & purification / Q000378:metabolism*; D000469:Alkaline Phosphatase / Q000302:isolation & purification / Q000378:metabolism*; D001713:Biphenyl Compounds / Q000494:pharmacology*; D002473:Cell Wall / Q000187:drug effects / Q000201:enzymology*; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D003904:Mitosporic Fungi / Q000201:enzymology*; D012248:Rhodotorula / Q000187:drug effects / Q000201:enzymology*
D001713:Biphenyl Compounds; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase
Etude des parois de levures Rhodotorula. VI. Influence du 2-hydroxybiphenyle sur les activités phosphatasiques de Rh. rubra