Properties of rat cerebral cortex neuronal nuclear surfaces: electrokinetic parameters.
Rat cerebral cortex neuronal nuclei were isolated by a mild technique utilizing sucrose; citric acid was not used in the isolation of the nuclei. These nuclei in 0.0145 M NaC1, 4.5 per cent sorbitol, and 0.6 mM NaHCO3 with pH 7.2 plus or minus 0.1 at 25 degrees C had an electrophoretic morbidity of minus 2.01 mum-s(-1)-V(-1)-cm(-1). The mobility curves for the brain nuclei indicated that the surface had an acid-dissociable group of pK APPROXIMATELY 2.7. Nuclei treated with 50 mg neurominidase/mg particle protein had a mobility of minus 1.4 mum-s(-1)-V(-1)-cm(-1). DNase or RNase at 50 mug/mg protein had no effect on the mobility of the isolated nuclei. Concanavalin A at 50 mug/mg protein decreased the nuclei electrophoretic mobility to minus 1.82 mum-s(-1)-V(-1)-cm(-1). The results are interpreted to mean that the brain nuclear external surface contains terminal sialic acid residues, but it is completely devoid of nucleic acids, and it binds canavalin A.
D000818:Animals; D002417:Cattle; D002467:Cell Nucleus / Q000032:analysis; D002540:Cerebral Cortex / Q000648:ultrastructure*; D003208:Concanavalin A / Q000494:pharmacology; D003851:Deoxyribonucleases / Q000494:pharmacology; D003864:Depression, Chemical; D004586:Electrophoresis; D006863:Hydrogen-Ion Concentration; D009439:Neuraminidase / Q000494:pharmacology; D009474:Neurons / Q000648:ultrastructure*; D051381:Rats; D012260:Ribonucleases / Q000494:pharmacology; D012794:Sialic Acids; D013268:Stimulation, Chemical
D012794:Sialic Acids; D003208:Concanavalin A; D003851:Deoxyribonucleases; D012260:Ribonucleases; D009439:Neuraminidase