Purification of soluble hepatocellular carcinoma extracts.
When soluble hepatocellular carcinoma (HCC) extract prepared by 3 M KCl solution was passed through a column of Sephadex G-200, four fractions (designated as I, II, III, IV) could be obtained from the elution profile. By leukocyte migration inhibition assay, the active component of soluble HCC extract which represented tumor-associated antigen was shown to be contained in fractions I and II.
D000328:Adult; D000951:Antigens, Neoplasm / Q000302:isolation & purification*; D006528:Carcinoma, Hepatocellular / Q000276:immunology*; D002464:Cell Migration Inhibition; D002850:Chromatography, Gel; D006801:Humans; D007962:Leukocytes / Q000276:immunology; D008113:Liver Neoplasms / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D008970:Molecular Weight; D012995:Solubility
D000951:Antigens, Neoplasm