Enzymes of the human intestinal brush border membrane. Identification after gel electrophoretic separation.
The postition of a number of human intestine brush border membrane enzyme activities in polyacrylamide gels after electrophoresis has been determined. These activities are, in order from the origin, maltase/glucoamylase, lactase/phlorizin hydrolase, maltase/sucrase/isomaltase, enteropeptidase, trehalase and gamma-glutamyl-transferase. Leucylnaphthylamide hydrolyzing activity was inactivated by sodium dodecylsulfate and its position was not determined. The positions of the activities have been correlated with the positions of protein bands previously determined. One such band situated between enteropeptidase and alkaline phosphatase has not been identified.
D000469:Alkaline Phosphatase / Q000032:analysis; D002462:Cell Membrane / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D006026:Glycoside Hydrolases / Q000032:analysis; D006801:Humans; D007421:Intestine, Small / Q000201:enzymology*; D008970:Molecular Weight; D010447:Peptide Hydrolases / Q000032:analysis; D011092:Polyethylene Glycols / Q000494:pharmacology; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013997:Time Factors; D005723:gamma-Glutamyltransferase / Q000032:analysis
D012967:Sodium Dodecyl Sulfate; D011092:Polyethylene Glycols; D005723:gamma-Glutamyltransferase; D000469:Alkaline Phosphatase; D006026:Glycoside Hydrolases; D010447:Peptide Hydrolases