Neutralization of aggregated strains of enterovirus 71 and echovirus type 4 in RD and Vero or GMK-AH1 cells.
Strains of enterovirus 71 and echovirus type 4 containing aggregates which were poorly neutralized by antibody in cell lines of green monkey kidney origin such as Vero and GMK-AH1, were as easily neutralized as non-aggregated strains when grown in human RD cells, The explanation for this may be a lesser degree of aggregation of viral materials originating from RD cells and/or the use of lower doses of virus for neutralization tests in such cells. The latter was a consequence of a higher sensitivity of RD cells to minimal amounts of virus than that shown by Vero and GMK-AH1 cells. Thus, virus grown in RD, Vero or GMK-AH1 cells reached a titre 10 to 100 times higher in RD cells than in the other cell lines.
D000818:Animals; D000914:Antibodies, Viral; D000956:Antigens, Viral; D002460:Cell Line; D004770:Enterovirus / Q000276:immunology*; D029822:Enterovirus B, Human / Q000276:immunology*; D000882:Haplorhini; D006801:Humans; D007106:Immune Sera; D007668:Kidney / Q000166:cytology; D009500:Neutralization Tests
D000914:Antibodies, Viral; D000956:Antigens, Viral; D007106:Immune Sera