Deoxyribonuclease in human parotid saliva.
Deoxyribonuclease (EC 3.1.4.5, EC 3.1.4.6) activities in human parotid saliva were examined by microdisc electrophoresis. Three fractions, differing in their electrophoretic mobility and in their optimal incubation conditions, were characterized. The various enzyme activities were tested at pH 5.0 in 100 mmol with l-minus 1 Na-acetate buffer or at pH 7.04 in 100 mmol with l-minus 1 Tris-HCl-buffer in the presence of different combinations of MgCl(2), CaCl(2), EDTA, and Na(2)SO(4).
D000328:Adult; D003851:Deoxyribonucleases / Q000378:metabolism*; D004589:Electrophoresis, Disc; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D010306:Parotid Gland / Q000201:enzymology* / Q000378:metabolism; D012463:Saliva / Q000201:enzymology*
D007527:Isoenzymes; D003851:Deoxyribonucleases