Characterization of human cytomegalovirus DNA: infectivity and molecular weight.
Human cytomegalovirus DNA was isolated from purified virions and further purified by sucrose density-gradient centrifugation. The viral DNA molecules were studied by electron microscopy and found to be linear and to have a length of 76.22 +/- 3.80 microM, corresponding to a molecular weight of 147.13 +/- 6.18 x 10(6). The DNA was infectious when tested in human embryonic lung cells using the DEAE-dextran and the calcium-phosphate techniques. The density in caesium chloride was 1.717 g/cm3.
D002472:Cell Transformation, Viral; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D003587:Cytomegalovirus / Q000502:physiology*; D004279:DNA, Viral / Q000008:administration & dosage / Q000302:isolation & purification*; D008168:Lung; D008970:Molecular Weight
D004279:DNA, Viral