Modified procedure for the recovery of naturally accumulated poliovirus from oysters.
Methods were compared for their ability to recover poliovirus from oysters (Crassostrea gigas) which had been allowed to accumulate virus via normal filtration activities. Clarification procedures included glycine-NaCl and polyelectrolyte extraction methods followed by a variety of acid precipitation concentration methods. Polyelectrolyte flocculation followed by a beef extract-supplemented acid precipitation carried out at pH 3.5 yielded the most efficient recoveries. Direct assay of homogenates was found to be an unreliable method for determining the initial virus concentration in "naturally infected" oysters.
D000818:Animals; D011232:Chemical Precipitation; D008828:Microbiological Techniques; D010125:Ostreidae / Q000382:microbiology*; D017955:Poliovirus / Q000302:isolation & purification*; D014462:Ultrafiltration; D014871:Water Microbiology