title: Quantitative determination of the lateral diffusion coefficients of the hormone-receptor complexes of insulin and epidermal growth factor on the plasma membrane of cultured fibroblasts.
abstract: Fluorescent derivatives of insulin and epidermal growth factor bound to 3T3 mouse fibroblasts are mobile on the cell surface, with similar diffusion coefficients, D approximately (3--5) x 10(-10) cm2/sec at 23 degrees C. Increasing the temperature to 37 degrees C results in rapid receptor immobilization. The immobilization is attributed to aggregation of hormone-receptor complexes, their internalization, or a combination of both processes.
mesh_terms: D001386:Azides / Q000494:pharmacology; D001667:Binding, Competitive; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D004815:Epidermal Growth Factor / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D005456:Fluorescent Dyes; D007328:Insulin / Q000378:metabolism*; D007700:Kinetics; D010455:Peptides / Q000378:metabolism*; D011972:Receptor, Insulin / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*
chemical_list: D001386:Azides; D005456:Fluorescent Dyes; D007328:Insulin; D010455:Peptides; D011956:Receptors, Cell Surface; D004815:Epidermal Growth Factor; D011972:Receptor, Insulin