title: Human erythrocyte glucose 6-phosphate dehydrogenase. Influence of coenzyme derivatives on thermostability and kinetic properties.
abstract: A number of derivatives of NADP(H) were tested with respect to their effectiveness in interacting with tetrameric glucose 6-phosphate dehydrogenase (G6PD) retaining only the fraction of "structural" coenzyme (4 moles NADP). Interaction was probed by two parameters: a) increased thermostability of G6PD activity, measured as the difference in the corresponding transition temperature (Tm) of samples containing and lacking the NADP derivatives, respectively; b) competitive inhibition toward NADP, expressed a Ki values. Protection afforded by the various effectors against thermal denaturation decreased in the following order: NADPH, NADP, PADP-ribose, adenosine 2',5'-P2. Other NADP derivatives, including 2',3' cyclic NADP, NMN, NMNH, nicotinamide, adenosine 2'-P, were uneffective in respect to this property. The kinetically measured affinity was the greatest for NADPH and decreased progressively for the following effectors: PADP-ribose, NADP, NMNH, PADP-glycolaldehyde, adenosine 2',5'-P2, PADP-ribitol, adenosine 2'-P. Nicotinamide and NMN were uneffective on NADP binding. These data show that the adenosine moiety of NADP is more critically involved than the nicotinamide portion in the interaction with human G6PD.
mesh_terms: D004355:Drug Stability; D004912:Erythrocytes / Q000201:enzymology*; D005954:Glucosephosphate Dehydrogenase / Q000097:blood* / Q000378:metabolism; D006358:Hot Temperature; D006801:Humans; D007700:Kinetics; D009249:NADP / Q000031:analogs & derivatives* / Q000494:pharmacology; D013329:Structure-Activity Relationship
chemical_list: D009249:NADP; D005954:Glucosephosphate Dehydrogenase