[Kinetic parameters of Na,K-ATPase in the gills of crabs adapted to freshened sea water].
The dependence of Na,K-ATPase activity on concentrations of ATP, Na+, K+, Mg2+ and ouabain in the membrane preparations of crab gills was studied. The first group of crabs was adapted to freshened (25%) and the second one--to normal (100%) sea water. A 40-day adaptation of crabs to the freshened sea water results in an increase of maximal activity of Na,K-ATPase, but does not affect the enzyme affinity for ATP, Na+, K+, Mg2+ and ouabain, as well as its cooperative properties. It is assumed that adaptation of crabs to freshened sea water is accompanied by an accumulation of Na, K-ATPase in the epithelial cell membranes or crab gills without causing any qualitative changes of the enzyme.
D000064:Acclimatization; D000818:Animals; D003386:Brachyura / Q000201:enzymology*; D005880:Gills / Q000201:enzymology*; D007700:Kinetics; D010042:Ouabain / Q000494:pharmacology; D012623:Seawater; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase
Kineticheskie parametry Na, K-ATPazy v zhabrakh krabov, adaptirovannykh k opresnennoĭ vode.