title: Determination of procaine and related local anesthetics. I. Partition chromatographic separation and assay of mixtures of procaine with tetracaine and with propoxycaine.
abstract: Determination of ionization and extraction constants for procaine, tetracaine, and propoxycaine led to selection of a simple partition chromatographic system for separation and assay of mixtures of these anesthetics. A 65% solution of chloroform in isooctane elutes tetracaine or propoxycaine from a pH 4:sodium bromide column; procaine is retained and subsequently eluted by chloroform as the bromide ion-pair. The anesthetics are then determined spectrophotometrically. Results of assay of standard and commercial formulations are presented.
mesh_terms: D000779:Anesthetics, Local / Q000592:standards; D002845:Chromatography; D004338:Drug Combinations; D006863:Hydrogen-Ion Concentration; D008722:Methods; D011343:Procaine / Q000032:analysis*; D011430:Propoxycaine / Q000032:analysis*; D012997:Solvents; D013053:Spectrophotometry; D013057:Spectrum Analysis; D013748:Tetracaine / Q000032:analysis*
chemical_list: D000779:Anesthetics, Local; D004338:Drug Combinations; D012997:Solvents; D013748:Tetracaine; D011343:Procaine; D011430:Propoxycaine