Radioactive labeling of proteins with an iodinated amidination reagent.
An iodinated phenolic imidoester has been synthesized for the labeling of proteins to high specific activities with radioactive iodine. The main advantage of this two-step labeling method is that it obviates direct contact of the protein with deleterious oxidizing agents, such as chloramine-T, pressent in direct methods of iodinating proteins.
D000818:Animals; D001555:Benzene Derivatives / Q000138:chemical synthesis; D004285:Dogs; D006863:Hydrogen-Ion Concentration; D007094:Imides / Q000138:chemical synthesis; D007202:Indicators and Reagents; D007457:Iodine Radioisotopes; D007553:Isotope Labeling / Q000379:methods*; D011506:Proteins; D012709:Serum Albumin; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature
D001555:Benzene Derivatives; D007094:Imides; D007202:Indicators and Reagents; D007457:Iodine Radioisotopes; D011506:Proteins; D012709:Serum Albumin