Complement-fixing immunoglobulin M antibody response in patients with infantile gastroenteritis.
Complement-fixing immunoglobulin M antibody to infantile gastroenteritis virus (a rotavirus) was detected with highest sensitivity when the antibody-antigen-complement mixture was incubated at 37 degrees C for 1 h prior to the addition of sensitized sheep erythrocytes. Sucrose gradient centrifugation of sera collected sequentially from four patients after infection detected 19S complement-fixing antibody up to 5 weeks, with highest titers at 1 week, after the onset of illness. Treatment of the whole sera with 2-mercaptoethanol decreased complement-fixing titers only up to 2 weeks after onset of illness.
D000914:Antibodies, Viral / Q000096:biosynthesis*; D003168:Complement Fixation Tests / Q000379:methods*; D005759:Gastroenteritis / Q000276:immunology*; D006801:Humans; D007075:Immunoglobulin M / Q000096:biosynthesis*; D007223:Infant; D012328:RNA Viruses / Q000276:immunology*; D012401:Rotavirus / Q000276:immunology*
D000914:Antibodies, Viral; D007075:Immunoglobulin M