title: Spontaneous rosette formation in vitro under the effect of agents affecting the cyclic nucleotide system of circulating lymphocytes of normal subjects.
abstract: Aminophylline was found to effect the early and total rosette-forming capacity of human thymus-dependent lymphocytes. This may be connected with an increase in the intracellular cyclic adenosine 3',5'-monophosphate level. Acetylcholine increases the proportion of early spontaneous rosettes, a phenomenon suggestive of a shift within the lymphocyte population toward a subpopulation of higher activity. The effect of acetylcholine is possibly mediated by an increase in the intracellular cyclic guanosine 3'-, 5'-monophosphate level. Aminophylline affects only the early rosette-forming capacity of thymus-derived lymphocytes. Levamisole has no influence on either early or total rosette-forming capacity of normal lymphocytes. The results are suggestive of definite relationships between the functional state, activity and cyclic nucleotide system of thymus-dependent lymphocytes.
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology*; D000628:Aminophylline / Q000494:pharmacology*; D000242:Cyclic AMP / Q000378:metabolism; D006152:Cyclic GMP / Q000378:metabolism; D004837:Epinephrine / Q000494:pharmacology*; D006801:Humans; D007978:Levamisole / Q000494:pharmacology; D009712:Nucleotides, Cyclic / Q000097:blood / Q000378:metabolism*; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
chemical_list: D009712:Nucleotides, Cyclic; D000628:Aminophylline; D007978:Levamisole; D000242:Cyclic AMP; D006152:Cyclic GMP; D000109:Acetylcholine; D004837:Epinephrine