title: Poly(adenosine diphosphate ribose) synthetase activity in nuclei of dividing and of non-dividing but differentiating intestinal epithelial cells.
abstract: Poly(ADP-ribose) synthetase activity is found in nuclei of regenerating epithelial cells in the lower half of the crypts of guinea-pig small intestine. Nuclei from non-dividing but differentiating and maturing cells in the upper crypts and on the villi contain no more than about 10% of the synthetase activity of lower-crypt cell nuclei. The product in the active nuclei is shown to be 80% poly(ADP-ribosylated) protein and 20% mono(ADP-ribosylated) protein; 60% of thetotal labelled product was attached to acid-soluble proteins (including histones), and 40% to acid-insoluble (non-histone) proteins. The average number of ADP-ribosyl units in the oligomeric chains of the poly(ADP-ribosylated) proteins was 15 but the range of sizes of (ADP-ribose) oligomers attached to nuclear proteins was smaller in villus than in crypt cell nuclei.
mesh_terms: D036002:ADP Ribose Transferases; D000818:Animals; D002454:Cell Differentiation; D002455:Cell Division; D002467:Cell Nucleus / Q000201:enzymology; D002469:Cell Separation; D004847:Epithelial Cells; D004848:Epithelium / Q000201:enzymology; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007583:Jejunum / Q000166:cytology / Q000201:enzymology*; D008297:Male; D009244:NAD+ Nucleosidase / Q000378:metabolism*; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*
chemical_list: D036002:ADP Ribose Transferases; D011065:Poly(ADP-ribose) Polymerases; D009244:NAD+ Nucleosidase