title: The in vitro biosynthesis of dhurrin, the cyanogenic glycoside of Sorghum bicolor.
abstract: A microsomal fraction from seedlings of Sorghum bicolor (Linn) Moench has been shown to catalyze the conversion of L-tyrosine to p-hydroxymandelonitrile via p-hydroxyphenylacetaldoxime. This transformation is consistent with the general pathway for cyanogenic glycoside biosynthesis proposed on the basis of in vivo experiments. When the microsomal fraction was combined with a protein fraction from the soluble portion of the cell and uridine diphosphate glucose, it was possible to demonstrate the synthesis of the cyanogenic glycoside dhurrin from L-tyrosine.
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D002854:Chromatography, Paper; D006027:Glycosides / Q000096:biosynthesis*; D009249:NADP; D009570:Nitriles / Q000378:metabolism*; D010084:Oxidation-Reduction; D010944:Plants / Q000378:metabolism*; D014443:Tyrosine / Q000378:metabolism; D014532:Uridine Diphosphate Glucose
chemical_list: D006027:Glycosides; D009570:Nitriles; D014443:Tyrosine; D009249:NADP; D014532:Uridine Diphosphate Glucose