Establishment of cell lines from the wild rodent Millardia meltada and tests for endogenous virus.
Four cell lines were established from the wild rodent, Millardia meltada; an untransformed cell line (MM-D), a non-producer (NP) cell line transformed with murine sarcoma virus (MM-CL4), and SV40-transformed cell lines (MM-8 MM-663). MM-D is epithelioid and contact-inhibited, whereas MM-CL4, MM-8, and MM-663 are fibroblastic and grow piling up. No endogenous virus was induced in Millardia cell lines by a long-term culture with various inducers. Millardia cell DNA has no nucleotide sequence homology with cDNA of either murine leukemia virus or rat endogenous virus.
D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral; D002875:Chromosomes; D004279:DNA, Viral / Q000032:analysis*; D004622:Embryo, Mammalian / Q000166:cytology / Q000382:microbiology; D009052:Leukemia Virus, Murine; D009693:Nucleic Acid Hybridization; D012190:Retroviridae; D012377:Rodentia / Q000382:microbiology*; D009053:Sarcoma Viruses, Murine; D013539:Simian virus 40
D004279:DNA, Viral