[Microspectrophotometric study of the crystalline inclusions in different strains of Amoeba proteus].
Using microspectrometric technique, integral extinction and extinction spectra of crystalline inclusions of two amoeba strains, C and Ct, have been studied. The analysis of extinction spectrum form allowed to suggest that the crystals seen in both amoeba strains have identical chemical composition, whereas the morphological differences between cells in their transparency may be due to different mode of the crystal package. The relative amount of the crystals was measured in both amoeba strains on the basis of the integral extinction value. The distinct differences between the strains were detected in the integral extinction value and in the extinction spectra, which allowed us to use these parameters as genetic markers in the experiments on the nuclear transplantation in amoeba.
D000656:Amoeba / Q000032:analysis*; D000818:Animals; D002999:Clone Cells / Q000032:analysis; D003460:Crystallization; D002479:Inclusion Bodies / Q000032:analysis*; D008832:Microchemistry / Q000379:methods; D013053:Spectrophotometry / Q000379:methods
Mikrospektrofotometricheskoe issledovanie kristallicheskikh vkliucheniĭ Amoeba proteus razlichnykh shtammov.