Ethoxyformylation of ribonuclease U2 form Ustilago sphaerogena.
RNase U2 was inactivated by incubation with ethoxyformic anhydride at pH 6.0 and pH 4.5.  The absorbance of the RNase U2 increased at around 250 nm and decreased at around 280 nm.  The inactivation occurred in parallel with the amount of modified histidine and plots of the relationship between the remaining activity and the modified histidine suggested that the modification of one of the two histidine residues totally inactivated the enzyme.  The inactivated enzyme RNase U2 was reactivated by a low concentration of hydroxyamine, with removal of the ethoxyformyl group from the modified histidine residue.  At pH 4.5, 2'-adenylate and 2'-guanylate protected RNase U2 from inactivation by ethoxyformic anhydride.  The difference CD spectra showed that the ability of RNase U2 to form a complex with 2'-adenylate was lost on ethoxyformylation.
D000215:Acylation; D000249:Adenosine Monophosphate; D001487:Basidiomycota / Q000201:enzymology*; D055598:Chemical Phenomena; D002621:Chemistry; D002845:Chromatography; D002942:Circular Dichroism; D004047:Diethyl Pyrocarbonate / Q000494:pharmacology*; D004789:Enzyme Activation; D005561:Formates / Q000494:pharmacology*; D006150:Guanine Nucleotides; D006639:Histidine / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D006898:Hydroxylamines; D012260:Ribonucleases / Q000032:analysis / Q000037:antagonists & inhibitors* / Q000378:metabolism; D014443:Tyrosine / Q000032:analysis; D014588:Ustilago / Q000201:enzymology*
D005561:Formates; D006150:Guanine Nucleotides; D006898:Hydroxylamines; D000249:Adenosine Monophosphate; D014443:Tyrosine; D006639:Histidine; D012260:Ribonucleases; D004047:Diethyl Pyrocarbonate