Mitochondrial and cytosolic localization of a single glycerate kinase in rat kidney cortex.
The distribution of glycerate kinase [ATP:D-glycerate 2-phosphotransferase, EC 2.7.1.31] in kidney was studied. This enzyme was found to be present in the renal cortex. By differential centrifugation of the homogenate and sucrose density gradient analysis, it was found that 42% and 60% of the renal glycerate kinase were localized in the cytosol and mitochondria, respectively. The mitochondrial enzyme appeared to be present in the inner membrane and/or matrix. No difference was found between the solubilized-mitochondrial and cytosolic glycerate kinase as regards kinetic properties, thermal stability, electrochemical properties, and molecular size. Immunochemical identity of these enzymes was demonstrated using a rabbit antibody against mitochondrial glycerate kinase purified from rat liver. Although the hepatic enzyme was induced by dietary protein (Kitagawa, Y., Katayama, H., & Sugimoto, E. [1979] Biochim. Biophys. Acta 582, 260--275), the renal enzyme in mitochondria and cytosol was not affected by dietary protein. These results on renal glycerate kinase are compared with those for the hepatic enzyme, and the regulatory mechanism for intracellular distribution of the enzymes is discussed.
D000818:Animals; D003600:Cytosol / Q000201:enzymology; D005988:Glyceric Acids; D007118:Immunoassay; D007425:Intracellular Membranes / Q000201:enzymology; D007672:Kidney Cortex / Q000201:enzymology*; D008297:Male; D008928:Mitochondria / Q000201:enzymology*; D010770:Phosphotransferases / Q000032:analysis*; D051381:Rats; D013347:Subcellular Fractions / Q000201:enzymology
D005988:Glyceric Acids; D010770:Phosphotransferases