Release of bound immunoglobulin from SSPE brain by acid elution.
SSPE brain homogenate extracted at pH 7.4 yields immunoglobulin with a 4- to 5-fold greater hemagglutination inhibition activity per microgram of IgG than serum from the same patient. Serial washing of the homogenate results in a low level steady-state release of IgG. Elution of the washed sediment with pH 2.5, 0.1 M glycine buffer results in a 2- to 3-fold increase in recovery of hemagglutination inhibition activity with a greater hemagglutination inhibition activity per milligram of IgG than the IgG recovered by phosphate-saline extraction at pH 7.4.
D001666:Binding Sites, Antibody; D001921:Brain; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000032:analysis / Q000302:isolation & purification*; D008459:Measles virus / Q000276:immunology; D011863:Radioimmunoassay; D011947:Receptors, Antigen, B-Cell / Q000302:isolation & purification*; D013344:Subacute Sclerosing Panencephalitis / Q000276:immunology*; D014020:Tissue Extracts / Q000276:immunology*
D007074:Immunoglobulin G; D011947:Receptors, Antigen, B-Cell; D014020:Tissue Extracts