title: [Estimation of methods for isolation of 3':5'-AMP-dependent protein kinases from animal tissues].
abstract: Different approaches are described to isolation and purification of soluble and membrane-bound 3':5'-AMP-dependent protein kinases from different tissues and intracellular structures. Main stages of the enzyme isolation: homogenization of tissues, acidic precipitation, fractionation by ammonium sulphate, chromatography on ion-exchangers and gel filtration, are characterized.
mesh_terms: D000818:Animals; D000242:Cyclic AMP / Q000494:pharmacology; D004789:Enzyme Activation; D005069:Evaluation Studies as Topic; D008566:Membranes / Q000201:enzymology; D008722:Methods; D011494:Protein Kinases / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D000242:Cyclic AMP; D011494:Protein Kinases
vernacular_title: Otsenka metodov vydeleniia 3':5'-AMP-zavisimykh proteinkinaz iz tkaneĭ zhivotnykh.