[Nucleotide specificity of rat liver thiamine pyrophosphokinase].
The nucleotide specificity of thiamine pyrophosphokinase from rat liver was studied. The enzyme was found to possess a sufficiently wide substrate specificity. Any of the nucleotides can be a donor of the pyrophosphate groups for TDP biosynthesis at two pH optima of the enzyme in the T-kinase reaction under the Mg2++/NTP optimal ratio. The minimal requirement for the substrate structure allowing to predict the position of the split nucleotide phosphoester bond was postulated.
D000818:Animals; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D010770:Phosphotransferases / Q000378:metabolism*; D051381:Rats; D013379:Substrate Specificity; D013836:Thiamin Pyrophosphokinase / Q000378:metabolism*
D010770:Phosphotransferases; D013836:Thiamin Pyrophosphokinase
Nukleotidnaia spetsifichnost' tiaminpirofosfokinazy iz pecheni krys.