[Study of sarcoplasmic reticulum Ca-ATPase by the paramagnetic label--paramagnetic probe method].
ESR spectra of nitroxyl derivative labelling with parachlormercury benzoate bound with SH-groups of Ca, Mg-dependent ATPase in the presence of Mn2+ ions are studied. It has been concluded from the saturation curves of ESR spectra that Mn2+ is localized at the distance approximately 40 A from the SH-group of the enzyme active centre. Platinum compound (K2PtCl4) changes the spectrum of ESR 1 due to the displacement of the label from the enzyme SH-groups and disintegration of the sarcoplasmic reticulum structure. Palladium compound (Pd(NH3)4Cl2) produced no effect on the ESR 1 spectrum.
D000818:Animals; D001665:Binding Sites; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D055598:Chemical Phenomena; D002621:Chemistry; D002729:Chloromercuribenzoates; D004578:Electron Spin Resonance Spectroscopy; D008274:Magnesium; D008345:Manganese; D010165:Palladium; D010984:Platinum; D011817:Rabbits; D012519:Sarcoplasmic Reticulum / Q000201:enzymology*
D002729:Chloromercuribenzoates; D008345:Manganese; D010984:Platinum; D010165:Palladium; D000252:Calcium-Transporting ATPases; D008274:Magnesium
Issledovanie Ca-ATFazy sarkoplazmaticheskogo retikuluma metodom paramagnitnaia metka--paramagnitnyĭ zond.