Survival of apurinic SV40 DNA in the d-complementation group of xeroderma pigmentosum.
The survival of depurinated Form I SV40 DNA was studied in normal human fibroblasts and in D-complementation Xeroderma pigmentosum (XP) fibroblasts. Survival was measured with an infective center assay. Heat-acid and methyl methanesulfonate (MMS) were used as depurinating agents. After 3 hrs of depurination by heat--acid treatment, infectivity in normal cells was less than 15% of the controls compared to more than 50% for the XP D cell strains. Similar results were obtained with MMS-treated DNA. These results are contrary to expectation since apurinic endonuclease activity, which is presumed to be involved in the repair of apurinic sites, is much lower in XP D cell strains than in normal cell strains. Our results indicate that another mechanism for the repair of apurinic sites could exist.
D002460:Cell Line; D004260:DNA Repair; D004279:DNA, Viral / Q000235:genetics*; D006801:Humans; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D009153:Mutagens; D013539:Simian virus 40 / Q000235:genetics*; D012867:Skin; D010948:Viral Plaque Assay; D014983:Xeroderma Pigmentosum / Q000235:genetics*
D004279:DNA, Viral; D009153:Mutagens; D008741:Methyl Methanesulfonate