Chorismate mutase-prephenate dehydrogenase from Aerobacter aerogenes: evidence that the two reactions occur at one active site.
The relationship between the sites for catalysis of two reactions by the bifunctional enzyme chorismate mutase--prephenate dehydrogenase has been investigated. The results are consistent with the occurrence of both reactions at one active site. Comparisons have been made between experimental data for the time course of the overall reaction and computer simulations, according to various models for the relationship between the mutase and dehydrogenase sites. A model based on a single active site is consistent with the time course data if a minor proportion of the chorismate that reacts can be converted through to (hydroxyphenyl)pyruvate without the intermediate release of prephenate. Consistent with this requirement, some channeling of radioactivity from chorismate to (hydroxyphenyl)pyruvate has been detected. A model based on two separate sites has also been considered; the simulations show that if this model applies there is no need to postulate any channeling of the intermediate, prephenate, between the sites and there must be marked inhibition of the dehydrogenase reaction by chorismate. Since channeling has been observed and chorismate increases the dehydrogenase rate under all conditions, the two-site model appears unlikely. Consistent with the one-site model are the observations that a variety of inactivating conditions cause parallel loss of mutase and dehydrogenase activity and that identical protection against inactivation of both mutase and dehydrogenase by iodoacetamide is afforded by prephenate.
D001665:Binding Sites; D002250:Carbon Radioisotopes; D002826:Chorismate Mutase / Q000378:metabolism*; D002827:Chorismic Acid; D004754:Enterobacter / Q000201:enzymology*; D004755:Enterobacteriaceae / Q000201:enzymology*; D007535:Isomerases / Q000378:metabolism*; D007553:Isotope Labeling; D007700:Kinetics; D008433:Mathematics; D009243:NAD; D010088:Oxidoreductases / Q000378:metabolism*; D011303:Prephenate Dehydrogenase / Q000378:metabolism*; D011485:Protein Binding
D002250:Carbon Radioisotopes; D009243:NAD; D010088:Oxidoreductases; D011303:Prephenate Dehydrogenase; D007535:Isomerases; D002826:Chorismate Mutase; D002827:Chorismic Acid