Solubility fo fibrin clots in monochloroacetic acid. A reflection of serum pepsinogen levels.
Fibrin clots formed from normal plasma dissolve readily in 1% monochloroacetic acid at 37 C. However, if the clots are washed thoroughly before the acid is added, they are no longer soluble. The agent present in the serum which causes dissolution of the fibrin clot was isolated and identified as pepsinogen. Because of the low pH of monochloroacetic acid the pepsinogen is activated and the clots are digested, simulating the dispersion of a fibrin clot which occurs in the absence of fibrin-stabilizing factor (factor XIII). Because of its higher pH, urea will not activate pepsinogen and is therefore a better agent to screen factor XIII deficiencies.
D000085:Acetates / Q000494:pharmacology*; D000818:Animals; D002417:Cattle; D002713:Chlorine / Q000494:pharmacology; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D003911:Dextrans; D004591:Electrophoresis, Polyacrylamide Gel; D005177:Factor XIII Deficiency / Q000175:diagnosis; D005342:Fibrinolysis / Q000187:drug effects*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D005779:Immunodiffusion; D010435:Pepsinogens / Q000097:blood / Q000302:isolation & purification* / Q000502:physiology; D013696:Temperature; D014508:Urea / Q000494:pharmacology
D000085:Acetates; D003911:Dextrans; D010435:Pepsinogens; D002713:Chlorine; C006972:chloroacetic acid; D014508:Urea