Apurinic and/or apyrimidinic endonuclease activity in ataxia telangiectasia cell extracts.
The possibility that the increased sensitivity of ataxia telangiectasia towards ionizing radiation is related to a DNA-repair deficiency has been examined further. When compared to unaffected controls, 6 lines of fibroblast cells derived from ataxia patients demonstrated a slightly reduced endonucleolytic activity (165 +/- 12 units vs. 214 +/- 28 units) towards apurinic and/or apyrimidinic sites as determined in a "nicking" assay.
D000135:Acid Phosphatase / Q000378:metabolism; D001260:Ataxia Telangiectasia / Q000201:enzymology*; D002460:Cell Line; D004260:DNA Repair; D004264:DNA Topoisomerases, Type I / Q000378:metabolism*; D004720:Endonucleases / Q000378:metabolism; D006801:Humans
D004720:Endonucleases; D000135:Acid Phosphatase; D004264:DNA Topoisomerases, Type I