Studies on the attachment of herpes simplex virus. Effect of trypsin treatment.
The adsorption of herpes simplex virus (HSV) to monolayers of trypsinized and mock-treated GMK-AH1 cells was studied by means of a microplaque technique. Plaque formation was nearly abolished for c:a 20 minutes and full sensitivity regained at 6--8 hours post trypsinization. The return of sensitivity to HSV was inhibited by cycloheximide and incubation at 4 degrees C. The sum of PFU in the supernatant and PFU successe modifying agents were without effect on the adsorption of HSV.
D000327:Adsorption; D000818:Animals; D002460:Cell Line; D003513:Cycloheximide / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D006026:Glycoside Hydrolases / Q000494:pharmacology; D000882:Haplorhini; D007668:Kidney; D011991:Receptors, Virus / Q000378:metabolism*; D018139:Simplexvirus / Q000378:metabolism*; D014357:Trypsin / Q000494:pharmacology; D014361:Trypsin Inhibitors / Q000494:pharmacology; D010948:Viral Plaque Assay
D011991:Receptors, Virus; D014361:Trypsin Inhibitors; D003513:Cycloheximide; D004492:Edetic Acid; D006026:Glycoside Hydrolases; D014357:Trypsin