Glutamine synthetase activity in WI-38 cells.
In the presence of complete growth media (Eagle's MEM), human diploid WI-38 cells have a low level of glutamine synthetase activity. The activity could be increased by depriving the cells of exogenous glutamine; addition of hydrocortisone to either glutamine-deficient or complete medium had no effect on the acitivty of the enzyme. Cell growth ceased under conditions that enhanced glutamine synthetase activity, and hydrocortisone could not reverse this inhibition.
D002455:Cell Division; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D005974:Glutamate-Ammonia Ligase / Q000096:biosynthesis*; D005973:Glutamine / Q000494:pharmacology; D006854:Hydrocortisone / Q000494:pharmacology
D005973:Glutamine; D005974:Glutamate-Ammonia Ligase; D006854:Hydrocortisone