Purification of influenza viruses on wide-pore glass columns.
Influenza viruses of different serotypes were purified by gel filtration on wide-pore glass. The virus thus purfied retained completely its haemagglutinating activity and infectivity, and by one purification cycle at least 99% of ballast proteins were removed.
D000327:Adsorption; D000468:Alkalies; D002850:Chromatography, Gel / Q000379:methods; D005898:Glass; D006851:Hydrochloric Acid; D006861:Hydrogen Peroxide; D006863:Hydrogen-Ion Concentration; D009975:Orthomyxoviridae / Q000302:isolation & purification*; D009994:Osmolar Concentration; D012965:Sodium Chloride / Q000494:pharmacology; D013242:Sterilization; D013997:Time Factors
D000468:Alkalies; D012965:Sodium Chloride; D006861:Hydrogen Peroxide; D006851:Hydrochloric Acid