[Isolation of surface antigens of influenza virus A/Hong Kong 1/68 (H3N2) by density gradient fractionation with a new carboxypolypeptidase (author's transl)].
Isolation of pure neuraminidase and hemagglutinin from influenza virus A/Hong Kong 1/68 (H3N2) can be achieved using the coupled action of a new carboxypolypeptidase from Streptomyces fradiae and sodium dodecyl sulfate (SDS). Biochemical, physical and physico-chemical studies showed that the two molecules are in pure form and of glycoproteic nature.
D002268:Carboxypeptidases / Q000378:metabolism; D002499:Centrifugation, Density Gradient / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D005971:Glutamates; D006389:Hemagglutinins, Viral / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D008854:Microscopy, Electron; D009439:Neuraminidase / Q000302:isolation & purification*; D009975:Orthomyxoviridae / Q000201:enzymology / Q000276:immunology* / Q000648:ultrastructure; D012967:Sodium Dodecyl Sulfate; D013055:Spectrophotometry, Infrared; D013302:Streptomyces / Q000201:enzymology; D013696:Temperature
D005971:Glutamates; D006389:Hemagglutinins, Viral; D012967:Sodium Dodecyl Sulfate; D009439:Neuraminidase; D002268:Carboxypeptidases
Séparation des antigènes de surface du virus A/Hong Kong 1/68 (H3N2) sur gradient de densité à l'aide d'une nouvelle carboxypolypeptidase