Studies of oligoadenylate formation on a poly (U) template.
We have studied a variety of condensation reactions involving poly (U) as template and isomeric adenosine dinucleotides as substrates. We find that [3'-5']-linked dinucleotides such as A3pA and pA3pA are better acceptors than the corresponding [2'-5']-linked compounds, while ImpA2pA is a better donor than ImpA3pA. The reaction between A2pA and ImpA3pA, for example, yields only 4% of product while the reaction of A3pA with ImpA2pA yields 86% of product. The more efficient condensation reactions of dimers are about as efficient as the self-condensation of ImpA. They yield a few percent of material in which five or more substrate molecules are linked together. The percentage of the natural [3'-5']-linkage in the product varies greatly, from as little as 1% to as much as 45%.
D002854:Chromatography, Paper; D004590:Electrophoresis, Paper; D009841:Oligonucleotides / Q000138:chemical synthesis*; D009843:Oligoribonucleotides / Q000138:chemical synthesis*; D010744:Phosphoric Monoester Hydrolases; D011072:Poly U; D011687:Purines; D013698:Templates, Genetic
D009841:Oligonucleotides; D009843:Oligoribonucleotides; D011687:Purines; D011072:Poly U; D010744:Phosphoric Monoester Hydrolases