Regions of the polyoma genome coding for T antigens.
The early region of the polyoma genome encodes three T antigens. We have analyzed the organization of the coding regions for the T antigens, using the nucleotide sequence of polyoma DNA and peptides derived from purified, radio-labeled T antigens, separated by two-dimensional electrophoresis and chromatography. We compared the peptides, predicted from the nucleotide sequence of the DNA, with those derived from the purified T antigens. We also compared chemically synthesized peptides, predicted from the DNA sequence, with observed peptides. The results show that the three polyoma T antigens are encoded in overlapping regions of the viral DNA, translated, in part, in two different reading frames.
D000956:Antigens, Viral; D001483:Base Sequence; D003545:Cysteine / Q000032:analysis; D004279:DNA, Viral / Q000378:metabolism; D005814:Genes, Viral; D005815:Genetic Code; D010446:Peptide Fragments / Q000032:analysis; D011120:Polyomavirus / Q000378:metabolism*; D014176:Protein Biosynthesis; D014158:Transcription, Genetic; D014357:Trypsin
D000956:Antigens, Viral; D004279:DNA, Viral; D010446:Peptide Fragments; D014357:Trypsin; D003545:Cysteine