Soluble and membrane-bound protein kinases in newborn and mature rat brain.
We studied the developmental changes of soluble and membrane-bound protein kinases (EC 2.7.1.37) in rat brain and found that the elution profiles from a DEAE-cellulose column by NaCl of adenosine-3':5'-monophosphate (cyclic AMP) dependent protein kinase from cytosol were not significantly different in newborn (0.19 M NaCl) vs. mature (0.21 M NaCl). In addition, there was little change in Kd for cyclic AMP binding of newborn and mature soluble enzyme. In contrast, using endogenous protein as the phosphate acceptor, cyclic AMP dependent and calcium-dependent protein kinases from brain synaptic membranes of mature rats were significantly higher than that of newborn rats.
D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D001921:Brain / Q000201:enzymology*; D002118:Calcium / Q000494:pharmacology; D000242:Cyclic AMP / Q000494:pharmacology; D003600:Cytosol / Q000201:enzymology; D008297:Male; D011494:Protein Kinases / Q000378:metabolism*; D051381:Rats; D013570:Synaptic Membranes / Q000201:enzymology
D000242:Cyclic AMP; D011494:Protein Kinases; D002118:Calcium