Thin-layer chromatographic methods to isolate 32P-labeled 5-phosphoribosyl-alpha-1-pyrophosphate (PRPP): determination of cellular PRPP pools and assay of PRPP synthetase activity.
D002855:Chromatography, Thin Layer / Q000379:methods; D004926:Escherichia coli / Q000201:enzymology; D007553:Isotope Labeling / Q000379:methods; D007700:Kinetics; D010428:Pentosephosphates / Q000032:analysis*; D010754:Phosphoribosyl Pyrophosphate / Q000032:analysis*; D010761:Phosphorus Radioisotopes; D010770:Phosphotransferases / Q000032:analysis*; D012268:Ribose-Phosphate Pyrophosphokinase / Q000032:analysis*; D012486:Salmonella typhimurium / Q000201:enzymology
D010428:Pentosephosphates; D010761:Phosphorus Radioisotopes; D010754:Phosphoribosyl Pyrophosphate; D010770:Phosphotransferases; D012268:Ribose-Phosphate Pyrophosphokinase