Fluorescence studies of the interaction between 1,N6-ethenoadenosine monophosphate and nucleotides.
AMP, GMP, TMP and CMP quench the fluorescence of 1,N6-ethenoadenosine monophosphate (epsilon-AMP). The fluorescence spectrum of epsilon-AMP-nucleotide system is identical with that of epsilon-AMP itself, and the fluorescence decay kinetics follow a single-exponential decay law. The dependence of fluorescence yields and lifetimes upon the concentration of nucleotides shows that the fluorescence of epsilon-AMP is principally quenched in a dynamic process by AMP, TMP and CMP, while it is quenched in both dynamic and static processes by GMP. The quenching constants increase in the following order: GMP greater than AMP greater than TMP greater than CMP.
D000249:Adenosine Monophosphate; D000255:Adenosine Triphosphate / Q000031:analogs & derivatives*; D055598:Chemical Phenomena; D002621:Chemistry; D003568:Cytidine Monophosphate; D004985:Ethenoadenosine Triphosphate / Q000031:analogs & derivatives*; D005453:Fluorescence; D006157:Guanosine Monophosphate; D009711:Nucleotides; D013938:Thymidine Monophosphate
D004985:Ethenoadenosine Triphosphate; D009711:Nucleotides; D013938:Thymidine Monophosphate; D000249:Adenosine Monophosphate; D006157:Guanosine Monophosphate; D000255:Adenosine Triphosphate; D003568:Cytidine Monophosphate