[Thymidylate synthetase in the antimesometrial and mesometrial portions of decidual tissue under normal conditions and following application of the antifolate preparation chloridine].
Specific activity of thymidylate synthetase (TMS) under normal conditions and after the action of pyrimethamine was investigated in antimesometrial (A) and mesometrial (M) parts of rat decidua. On the 9th-11th days of pregnancy specific activity of TMS in the A part proved to be higher than in the M part of decidua, and the former one reached its maximum by the 10th day. Specific activity of TMS in the M part decreased gradually from the 9th to 11th days of gestation. Pyrimethamine, applied on the 9th day of pregnancy caused a wave-like increase in the specific activity of TMS in the A part; this elevation was not so pronounced in the M part; A study of the physico-chemical properties of TMS from the decidua and the embryos of rats showed that the enzyme had a molecular weight of 58000, an optimal pH of 6.9, and could be quickly inactivated by heating.
D000818:Animals; D002384:Catalysis; D055598:Chemical Phenomena; D002621:Chemistry; D002850:Chromatography, Gel; D003656:Decidua / Q000201:enzymology*; D005260:Female; D005865:Gestational Age; D006863:Hydrogen-Ion Concentration; D008780:Methyltransferases / Q000032:analysis*; D008970:Molecular Weight; D011247:Pregnancy; D011739:Pyrimethamine / Q000494:pharmacology*; D051381:Rats; D013940:Thymidylate Synthase / Q000032:analysis*; D013997:Time Factors
D008780:Methyltransferases; D013940:Thymidylate Synthase; D011739:Pyrimethamine
Issledovanie timidilatsintetazy v antimezometralńoĭ i mezometralńoĭ chastiakh detsidualńoĭ tkani v norme i pri deĭstvii antifolievogo preparata khloridina.