Increase in Na+, K+ -ATPase enzyme units in liver and kidneys from essential fatty acid deficient rats.
(3H)-Ouabain binding to liver and kidney preparations was utilized to estimate the number of Na+, K+-ATPase enzyme units in livers and kidneys from rats fed 2% corn oil supplemented or fat-free diets. The specific (3H)-ouabain binding in liver and kidney preparations from fatty acid deficient rats was increased approximately 40%, but the affinity of the binding sites for ouabain (Kd-value) remained unchanged. The increased concentration of Na+, K+-ATPase enzyme units observed in the essential fatty acid deficient rats may contribute to the reduced body fat accumulation and elevated heat production observed in these animals.
D000818:Animals; D001665:Binding Sites; D001835:Body Weight; D004041:Dietary Fats / Q000378:metabolism; D004734:Energy Metabolism; D005228:Fatty Acids, Essential / Q000172:deficiency*; D007668:Kidney / Q000201:enzymology*; D008099:Liver / Q000201:enzymology*; D008297:Male; D010042:Ouabain / Q000378:metabolism; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
D004041:Dietary Fats; D005228:Fatty Acids, Essential; D010042:Ouabain; D000254:Sodium-Potassium-Exchanging ATPase