Time saving protein binding assay for the simultaneous determination of guanosine 3':5'-monophosphate (cGMP) and adenosine 3':5'-monophosphate (cAMP) in human urine.
A time-saving protein binding assay for the simultaneous determination of cGMP and cAMP has been adapted for human urine, using [3H]cGMP, [14C]cAMP, protein fractions from calf skeletal and lobster tail muscles and the phosphodiesterase inhibitor SQ 20.009. Recovery, accuracy, and precision are approximately at the 10% limit. Good specificity and no interference were observed with diluted urine samples (10 to 20 times).
D000818:Animals; D002021:Buffers; D002417:Cattle; D000242:Cyclic AMP / Q000652:urine*; D006152:Cyclic GMP / Q000652:urine*; D006801:Humans; D008722:Methods; D009124:Muscle Proteins; D008121:Nephropidae; D010726:Phosphodiesterase Inhibitors; D011485:Protein Binding
D002021:Buffers; D009124:Muscle Proteins; D010726:Phosphodiesterase Inhibitors; D000242:Cyclic AMP; D006152:Cyclic GMP