Localization of a gene for human alpha-galactosidase B (= n-acetyl-alpha-d-galactosaminidase) on chromosome 22.
The localization of the structural gene for human alpha-galactosidase B (= N-acetyl-alpha-galactosaminidase) was investigated by means of man-Chinese hamster and man-mouse somatic cell hybrids. The hybrid clones were analyzed for chromosomes and for a large number of known enzyme markers. The lysates of the hybrid cells were treated with Sepharose-coupled antihuman alpha-galactosidase B and the activity of the adsorbed enzyme was measured on the Sepharose beads as N-acetyl-alpha-galactosaminidase. The results show that the structural gene for human alpha-galactosidase B is situated on chromosome 22, and that there is no structural relationship between human alpha-galactosidase A and human alpha-galactosidase B.
D000818:Animals; D002874:Chromosome Mapping; D002904:Chromosomes, Human, 21-22 and Y; D006224:Cricetinae; D005696:Galactosidases / Q000235:genetics*; D005796:Genes; D006801:Humans; D006822:Hybrid Cells / Q000201:enzymology*; D007158:Immunologic Techniques; D000519:alpha-Galactosidase / Q000235:genetics*
D005696:Galactosidases; D000519:alpha-Galactosidase