title: Resolution of cytochrome oxidase into two component complexes.
abstract: Cytochrome c oxidase (ferrocytochrome c: oxygen oxidoreductase, EC 1.9.3.1) has been resolved into a pair of complexes of unequal molecular weight. The larger complex (electron transfer complex) contains exclusively the oxidation-reduction proteins characteristic of cytochrome oxidase; the smaller complex (ion transfer complex) shows exclusively the capability for cation-dependent induction of the fluorescence of 8-anilino-1-naphthalenesulfonic acid--a capability demonstrable in preparations of cytochrome oxidase. The duplex nature of cytochrome oxidase has important implications for the mechanism of energy coupling.
mesh_terms: D003300:Copper / Q000032:analysis; D004579:Electron Transport; D003576:Electron Transport Complex IV / Q000302:isolation & purification / Q000378:metabolism*; D004734:Energy Metabolism; D004735:Energy Transfer; D006418:Heme / Q000032:analysis; D007477:Ions; D046911:Macromolecular Substances; D008970:Molecular Weight; D013057:Spectrum Analysis; D013329:Structure-Activity Relationship
chemical_list: D007477:Ions; D046911:Macromolecular Substances; D006418:Heme; D003300:Copper; D003576:Electron Transport Complex IV