The influence of growth temperature on the heat stability of inorganic pyrophosphatase from crude extracts of Bacillus stearothermophilus.
Inorganic pyrophosphatase activity of crude extracts from Bacillus stearothermophilus adapts its thermostability to the growth temperature of the cells. Magnesium ions increase the stability of the enzyme in all cases. Depending on the growth temperature of the cells two pyrophosphatase species differing in their molecular weights and heat stabilities have been detected.
D004355:Drug Stability; D001411:Geobacillus stearothermophilus / Q000201:enzymology* / Q000254:growth & development; D006358:Hot Temperature; D007527:Isoenzymes / Q000032:analysis; D008274:Magnesium / Q000494:pharmacology; D008970:Molecular Weight; D011487:Protein Conformation; D011755:Pyrophosphatases / Q000032:analysis / Q000378:metabolism*; D013696:Temperature
D007527:Isoenzymes; D011755:Pyrophosphatases; D008274:Magnesium