Regulation of sterol synthesis in human lymphocytes: evidence for post-transcriptional control by low density lipoprotein.
The effect of cordycepin (3'-deoxyadenosine), an inhibitor of messenger RNA synthesis, on the induction of 3-hydroxy-3-methylglutaryl coenzyme A reductase mediated by lipid-depleted serum was studied in isolated human lymphocytes. 50 micrograms/ml cordycepin, although inhibiting messenger RNA synthesis by more than 50%, had no inhibitory effect on the two and four-fold induction of hydroxymethylglutaryl-CoA reductase when cells were incubated in a medium containing lipid-depleted serum for 8 and 16 h, respectively. This result suggests that newly synthesises messenger RNA is not required for the effect of lipid-depleted serum on the induction of hydroxymethylglutaryl-CoA reductase in human lymphocytes.
D003839:Deoxyadenosines / Q000494:pharmacology; D006801:Humans; D006903:Hydroxymethylglutaryl CoA Reductases / Q000378:metabolism; D066298:In Vitro Techniques; D008077:Lipoproteins, LDL / Q000502:physiology*; D008214:Lymphocytes / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D013261:Sterols / Q000096:biosynthesis*
D003839:Deoxyadenosines; D008077:Lipoproteins, LDL; D012333:RNA, Messenger; D013261:Sterols; D006903:Hydroxymethylglutaryl CoA Reductases