[Ornithine transcarbamylase in Clostridium perfingens].
Five strains of A type Cl. perfringens of different titres of toxicity (BP6K, SR-12, 2836, 2910, 1) possessed a distinct activity of ornithine transcarbamylase. In cells the maximal activity of the enzyme was observed within 3-5 hrs of growth of the culture. The enzyme was partially purified and some of its physico-chemical properties were studied. Ornithine transcarbamylase was termostable. Monoiodine acetate, p-chloromercurybenzoate, semicarbazide, Hg-2+, Cu-2+ and Fe-3+ inhibited the enzymatic activity, but Mg-2+ and Ca-2+ activated the enzyme. Ornithine transcarbamylase was shown to be active in wide region of pH: from pH 6.0 to pH 9.0 and higher. Two peaks of the enzyme activity were observed: the first (a more distinct one) at pH 8.6-8.9 and the second, smaller,--at pH 6.5-6.7.
D002458:Cell Fractionation; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D002956:Citrulline / Q000096:biosynthesis; D003016:Clostridium perfringens / Q000201:enzymology* / Q000472:pathogenicity; D003470:Culture Media; D004355:Drug Stability; D004789:Enzyme Activation / Q000187:drug effects; D004794:Enzyme Repression / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D009954:Ornithine Carbamoyltransferase / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors; D014465:Ultrasonics
D003470:Culture Media; D002956:Citrulline; D009954:Ornithine Carbamoyltransferase
Ornitintranskarbamilaza Clostrium perfringens