Persistent vesicular stomatitis virus infection mediates base substitutions in viral RNA termini.
We have sequenced (via a product RNA) the 3' RNA terminus of a defective interfering particle that was generated from the standard virus isolated from a culture of BHK-21 cells persistently infected with vesicular stomatitis virus for over 5 years. By hybridization and RNA sequencing, seven mutations were identified in the 46 nucleotides at the terminus of this defective-interfering-particle RNA. It is likely that these mutations are a reflection of altered protein-nucleic acid interactions that the virus has evolved to maintain its persistently infected carrier state in vitro.
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D006224:Cricetinae; D003673:Defective Viruses / Q000032:analysis* / Q000254:growth & development; D007668:Kidney; D009154:Mutation; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000032:analysis*; D014721:Vesicular stomatitis Indiana virus / Q000032:analysis* / Q000254:growth & development
D012367:RNA, Viral