Persistent infection of human lymphoid and myeloid cell lines with herpes simplex virus.
Herpes simplex virus (HSV) type 1 replicated and persisted in human T, B, and myeloid cell lines with different patterns of viral replication and various effects on cell growth. T cell line CEM supported the replication of HSV for over 400 days without detectable differences in cell growth as compared with uninfected cells. HSV persisted in B cell line NC37 and myeloid cell line K562 for up to 222 and 374 days, respectively, but led to a significant decrease in the number of viable cells by 7 weeks of infection. The average number of cells producing infectious virus was very low in these cell lines (range, 0.5 to 2.7+) compared with a larger proportion of cells exhibiting HSV antigens by immunofluorescence (range, 24 to 58%). In contrast, null cell line LAZ 221 failed to replicate HSV even though the viral infection led to a cessation of cell growth.
D001402:B-Lymphocytes / Q000382:microbiology*; D001853:Bone Marrow / Q000382:microbiology*; D002460:Cell Line; D006561:Herpes Simplex / Q000382:microbiology*; D006801:Humans; D018139:Simplexvirus / Q000254:growth & development*; D013601:T-Lymphocytes / Q000382:microbiology*; D013997:Time Factors; D014779:Virus Replication