title: Ligand binding properties of hemoglobin 3 of the trout, Salmo gairdneri. The occurrence of an acid Bohr effect in the absence of heme-heme interaction.
abstract: The four components of hemoglobin from the rainbow trout (Salmo gairdneri) have been isolated. The oxygen affinities of the first two components eluted from the DEAE-cellulose column have much smaller pH dependencies than the last two components. These components have very low O2 affinities at low pH. The effect of pH on the equilibrium and kinetics of ligand binding to the third fraction, the pH-dependent component present in greatest amounts, has been studied. Measurements of ligand binding equilibria demonstrate the presence of both an alkaline and an acid Bohr effect. In the region of the alkaline Bohr effect the value of n in the Hill equation is a function of ligand affinity. For CO binding n decreases as the pH is decreased until at pH 6, the minimum ligand affinity is reached. At this pH there is also a complete loss of cooperative ligand binding. Decreasing the pH further results in an increase of ligand affinity, but this acid Bohr effect is not associated with a reappearance of cooperativity. This suggests that Fraction 3 of S. gairdneri is frozen in the low affinity, deoxygenated conformation at low pH and that the quaternary structure does not change even when fully liganded. However, the properties of the low affinity conformation of this hemoglobin are pH-dependent.
mesh_terms: D000818:Animals; D001665:Binding Sites; D002263:Carboxyhemoglobin; D002848:Chromatography, DEAE-Cellulose; D006418:Heme; D006454:Hemoglobins; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008024:Ligands; D046911:Macromolecular Substances; D009569:Nitric Oxide; D010100:Oxygen / Q000097:blood*; D010777:Photochemistry; D011485:Protein Binding; D012487:Salmonidae / Q000097:blood*; D013997:Time Factors; D014337:Trout / Q000097:blood*
chemical_list: D006454:Hemoglobins; D008024:Ligands; D046911:Macromolecular Substances; D009569:Nitric Oxide; D006418:Heme; D002263:Carboxyhemoglobin; D010100:Oxygen