title: Biochemical characterization of lysosomes in unfertilized eggs of Xenopus laevis.
abstract: Relations between lysosomes and yolk platelets of amphibian eggs have been suggested. This work demonstrates the presence of acid hydrolases in oocytes induced to ovulate in vitro. About 40% of the acid hydrolases are found in a sedimentable fraction, and, in accordance with the lysosomal concept, they display structural latency. Biochemical data did not indicate any association between lysosomal enzymes and yolk platelets. The mechanism of yolk resorption is discussed and it is suggested that the fusion of lysosomes and yolk platelets might be one of the mechanisms involved in yolk digestion.
mesh_terms: D000118:Acetylglucosaminidase / Q000378:metabolism; D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D001001:Anura; D002499:Centrifugation, Density Gradient; D003576:Electron Transport Complex IV / Q000378:metabolism; D005260:Female; D006867:Hydrolases / Q000378:metabolism*; D008247:Lysosomes / Q000201:enzymology*; D009865:Oocytes / Q000201:enzymology*; D010063:Ovum / Q000201:enzymology*; D010447:Peptide Hydrolases / Q000378:metabolism; D014981:Xenopus / Q000378:metabolism*
chemical_list: D003576:Electron Transport Complex IV; D006867:Hydrolases; D000135:Acid Phosphatase; D000118:Acetylglucosaminidase; D010447:Peptide Hydrolases