Enhanced proliferation of endogenous virus in Chinese hamster cells associated with microtubules and the mitotic apparatus of the host cell.
Chinese hamster ovary cells harbour intracytoplasmic virus-like particles of type A which are closely associated with sites of microtubule formation. We report here the enhanced proliferation of these particles and their release at the cell membrane by using either 5-bromodeoxyuridine or dibutyryl cyclic AMP. The extracellular mature particles are similar in morphology to retroviruses of type B. Close association of the type A virus precursors with microtubule organizing centres, i.e. kinetochores, centrioles and basal bodies, and with microtubules per se, is confirmed by studying the effects of the microtubule inhibitors Colcemid and vincristine sulphate. The role of microtubules in the activation and transport of the intracytoplasmic type A particles is discussed.
D000818:Animals; D001973:Bromodeoxyuridine / Q000494:pharmacology; D003994:Bucladesine / Q000494:pharmacology; D002460:Cell Line; D002502:Centrioles / Q000382:microbiology*; D003078:Colchicine / Q000494:pharmacology; D006224:Cricetinae; D003412:Cricetulus; D003907:Dexamethasone / Q000494:pharmacology; D005260:Female; D008870:Microtubules / Q000382:microbiology*; D008938:Mitosis; D009940:Organoids / Q000382:microbiology*; D010053:Ovary; D012190:Retroviridae / Q000254:growth & development*; D014750:Vincristine / Q000494:pharmacology; D014779:Virus Replication / Q000187:drug effects
D014750:Vincristine; D003994:Bucladesine; D003907:Dexamethasone; D001973:Bromodeoxyuridine; D003078:Colchicine