Thiourea derivatives as specific inhibitors of picorna viruses.
30 compounds with antipicorna virus activity were selected from 173 N,N'-disubstituted thiourea derivatives. The spectrum of antiviral activity was determined in vitro using entero, FMD, rhino and EMC viruses. Structure-activity relationships were studied. Several compounds produced marked activity against coxsackie viruses A and B infection of mice and FMD infection in mice and guinea pigs. N-Phenyl-N'-3-hydroxyphenyl-thiourea (PTU-23) inhibited poliovirus production by more than 99% without influencing the FL host cell. EMC virus was readily inhibited by PTU-23 in Kreb-II cells. Virus RNA synthesis was significantly reduced. Under the effect of PTU-23 the amount of 37S ssRNA extracted from EMC virus infected cells was considerably more reduced than that of the 20S ds RNA. PTU-23 did not influence the activity of virus-induced RNA polymerase in a cell-free system.
D000818:Animals; D000998:Antiviral Agents; D004353:Drug Evaluation, Preclinical; D051379:Mice; D010849:Picornaviridae / Q000187:drug effects* / Q000254:growth & development; D010850:Picornaviridae Infections / Q000188:drug therapy; D012324:RNA-Dependent RNA Polymerase / Q000037:antagonists & inhibitors; D013890:Thiourea / Q000031:analogs & derivatives* / Q000494:pharmacology; D013997:Time Factors
D000998:Antiviral Agents; D012324:RNA-Dependent RNA Polymerase; D013890:Thiourea