Degradation of feather keratin by culture filtrates of Streptomyces fradiae.
Treatment of milled defatted capon feathers with filtrates of Steptomyces fradiae ATCC 10745 that had been concentrated 20 times showed that the most keratin degradation in 0.5h occurred at pH 8.0 and 60degrees C. If assay time was extended, however, adecrease in reaction rate occurred and appeared to result from keratinase instability at elevated temperatures. Exposure of commercial feather meal to unconcentrated culturefiltrates for 15 h produced greatest degradation at 50 degrees C.
D000818:Animals; D002645:Chickens; D005241:Feathers; D006863:Hydrogen-Ion Concentration; D006867:Hydrolases / Q000378:metabolism*; D007633:Keratins / Q000378:metabolism*; D007700:Kinetics; D013302:Streptomyces / Q000201:enzymology*; D013696:Temperature
D007633:Keratins; D006867:Hydrolases