title: Liquid chromatography in pharmaceutical analysis IV: determination of antispasmodic mixtures.
abstract: Parameters associated with the separation of antianxiety-antispasmodic agents were investigated using high-pressure liquid chromatography. Eight widely prescribed drugs were studied. The compounds were chromatographed on reversed-phase octadecyltrichlorosilane (C18) or diphenyldichlorosilane (phenyl) columns, using mixtures of absolute methanol and distilled water buffered with ammonium dihydrogen phosphate, ammonium acid phosphate, or ammonium carbonate. A mixture of phenobarbital-propantheline bromide was selected to demonstrate the utility of the separation and quantification method. The mixture was chromatographed on a phenyl column, using absolute methanol-aqueous 1 percent ammonium dihydrogen phosphate (60:40) (pH 5.85) at a flow rate of 1.4 ml/min. Each determination can be achieved in approximately 15 min with an accuracy of 1-2 percent.
mesh_terms: D001285:Atropine / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D002707:Chlordiazepoxide / Q000032:analysis; D002845:Chromatography; D004338:Drug Combinations; D006863:Hydrogen-Ion Concentration; D008722:Methods; D010276:Parasympatholytics / Q000032:analysis*; D010634:Phenobarbital / Q000032:analysis; D011346:Prochlorperazine / Q000032:analysis; D011413:Propantheline / Q000032:analysis; D000644:Quaternary Ammonium Compounds / Q000032:analysis; D011812:Quinuclidines / Q000032:analysis; D012601:Scopolamine / Q000032:analysis
chemical_list: D004338:Drug Combinations; D010276:Parasympatholytics; D000644:Quaternary Ammonium Compounds; D011812:Quinuclidines; D011413:Propantheline; D002707:Chlordiazepoxide; D001285:Atropine; D012601:Scopolamine; D011346:Prochlorperazine; D010634:Phenobarbital