Improved method for cytogenetic studies of solid tumors.
Solid human tumors were dissociated with collagenase, cultured for 16-48 hours, and harvested for cytogenetic preparation. Of the 19 tumors used, 14 showed sufficient numbers of metaphases to be useful for chromosome analysis.
D002871:Chromosome Banding; D003470:Culture Media; D005260:Female; D005833:Genital Neoplasms, Female / Q000235:genetics; D006801:Humans; D007621:Karyotyping / Q000379:methods*; D008677:Metaphase; D003012:Microbial Collagenase; D009369:Neoplasms / Q000235:genetics*
D003470:Culture Media; D003012:Microbial Collagenase