[Peculiarities of phosphorylation of skeletal muscle troponin under some forms muscle pathologies].
Phosphorylation of rat and rabbit troponin from normal skeletal muscles and from skeletal muscles of animals under avitaminosis, denervation and hypokinesia was studied. Phosphorylation was carried out by cAMP-dependent protein kinase with [gamma-33P] as substrate. The incorporation of labelled phosphorus into troponin T of the damaged muscles was decreased as compared to normal. After preliminary dephosphorylation of troponin by alkaline phosphatase immobilized on Sepharose 4B, the ability of damaged muscle troponin for subsequent phosphorylation was also decreased as compared to the control. It may be thus assumed that there exist conformational changes of troponin under muscular system pathologies.
D000469:Alkaline Phosphatase; D000818:Animals; D001361:Avitaminosis / Q000378:metabolism*; D000242:Cyclic AMP / Q000494:pharmacology; D004789:Enzyme Activation; D007700:Kinetics; D009121:Muscle Denervation; D009124:Muscle Proteins / Q000378:metabolism*; D009132:Muscles / Q000378:metabolism* / Q000473:pathology; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D011817:Rabbits; D051381:Rats; D014336:Troponin / Q000378:metabolism*
D009124:Muscle Proteins; D014336:Troponin; D000242:Cyclic AMP; D011494:Protein Kinases; D000469:Alkaline Phosphatase
Osobennosti fosforilirovaniia troponina skeletnykh myshts pri nekotorykh formakh patologii myshechnoĭ sistemy.