title: Factitious changes in binding of oxygen to hemoglobin when based on extracellular pH in the presence of certain blood additives like radiographic contrast media.
abstract: Construction of oxygen-hemoglobin DISSOCIATION CURVES BASED ON EXTRACELLULAR PH and using blood tonometered with 5 per cent CO2, is misleading under certain experimental conditions.  These include the presence in blood of poorly penetrating non-ionic molecules like sucrose of poorly penetrating anionic aompounds like radiographic contrast materials.  False conclusions regarding the position of the oxygen-hemoglobin dissociation curve can result because of the disturbance of the normal pH gradient between plasma and red cell induced by such chemicals.
mesh_terms: D001769:Blood; D003287:Contrast Media; D003973:Diatrizoate; D004912:Erythrocytes; D006454:Hemoglobins; D006801:Humans; D006863:Hydrogen-Ion Concentration; D010100:Oxygen / Q000097:blood*; D011485:Protein Binding; D013395:Sucrose; D014680:Veins
chemical_list: D003287:Contrast Media; D006454:Hemoglobins; D003973:Diatrizoate; D013395:Sucrose; D010100:Oxygen