[NAD+ synthesis in the isolated kidney cell nuclei of dogs after the intravenous administration of amphotericin B].
A study was made of the synthesis of nicotinamide adenine dinucleotide (NAD+) in the nuclei of kidney cells of dogs under normal conditions and upon the effect of the polyenic antibiotic amphotericin B. An active NAD-pyrophosphorylase has been found in the nuclei of kidney cells. It has been established that a intervenous introduction of amphotericin B stimulates NAD+ production. Amphotericin B also causes a decrease in the amount of histones in the nucleus. In the case of the nuclear membrane damage by a non-ionic detergent Triton X-100, no increase in the synthesis of NAD+ has been observed in the nuclei of kidney cells of animals treated with antibiotics, as opposed to the control ones. Under discussion is a question of a possible mechanism of the effect of polyenic antibiotics on the synthesis and metabolic activity of NAD+.
D000666:Amphotericin B / Q000494:pharmacology*; D000818:Animals; D002414:Cations, Monovalent; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism*; D004285:Dogs; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D006657:Histones / Q000378:metabolism; D007275:Injections, Intravenous; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D009243:NAD / Q000096:biosynthesis*; D009612:Nicotinamide-Nucleotide Adenylyltransferase / Q000378:metabolism
D002414:Cations, Monovalent; D006657:Histones; D009243:NAD; D000666:Amphotericin B; D009612:Nicotinamide-Nucleotide Adenylyltransferase
Sintez NAD+ v izolirovannykh iadrakh kletok pochek sobak posle vnutrivennogo vvedeniia amfoteritsina B.