[In-vitro inhibition of collagenase activity in epidermolysis bullosa hereditaria dystrophica].
By means of a biological collagenase assay we estimated collagenolytic activity of the vesicular fluid of patients with epidermolysis bullosa hereditaria dystrophica. All the 5 examined patients showed increased collagenolysis. Inhibition studies were performed applying ethylendiaminetetraacetate inhibition 11,15%, normal human serum in 12.15% (means). o-Phenanthroline as well as erythromycine in an aequimolar ratio stopped the collagenolytic activity completely in the 5 specimen. It was the aim of our study to indicate that erythromycine, in contrast to other collagenase inhibitors relatively atoxic, is able to inhibit collagenase activity which is suggested to play a pathogenetic role in that disease.
D000328:Adult; D001681:Biological Assay; D004492:Edetic Acid / Q000494:pharmacology; D004820:Epidermolysis Bullosa / Q000201:enzymology*; D004917:Erythromycin / Q000494:pharmacology; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D007223:Infant; D008297:Male; D003012:Microbial Collagenase / Q000037:antagonists & inhibitors*; D010618:Phenanthrolines / Q000494:pharmacology
D010618:Phenanthrolines; D004917:Erythromycin; D004492:Edetic Acid; D003012:Microbial Collagenase
In vitro-Hemmung der Kollagenaseaktivität bei Epidermolysis bullosa hereditaria dystrophica.