Studies on the mechanism of metabolic stimulation in polymorphonuclear leucocytes during phagocytosis. II. Presence of the NADPH2 oxidizing activity in a myeloperoxidase-deficient subject.
1. The biochemical properties of leucocytes from a myeloperoxidase-deficient subject were compared with those of leucocytes from healthy subjects. 2. Myleoperoxidase-deficient leucocytes responded to phagocytosis of heat-killed bacteria with increased respiration, increased oxidation of glucose through the hexose monophosphate shunt and increased production of H2O2 as normal leucocytes do. 3. The ability of granules isolated from myeloperoxidase-deficient leucocytes to oxidize nicotinamide coenzymes was comparable to that of granules isolated from normal leucocytes. 4. The results argue against the hypothesis that oxidation of NADPH2 in leucocytes is performed by myeloperoxidase.
D000328:Adult; D003594:Cytoplasmic Granules / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006098:Granulocytes / Q000378:metabolism; D006600:Hexosephosphates / Q000378:metabolism; D006801:Humans; D007962:Leukocytes / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D008661:Metabolism, Inborn Errors / Q000097:blood*; D009249:NADP / Q000378:metabolism; D010544:Peroxidases / Q000172:deficiency* / Q000378:metabolism; D010587:Phagocytosis
D006600:Hexosephosphates; D009249:NADP; D010544:Peroxidases; D005947:Glucose