Chromosomal mobilization from a recA mutant of Pseudomonas putida.
A methyl methane sulfonate sensitive mutant of P. putida strain PpG1 is also extremely sensitive to UV-rays, compared to parent wild type cells. This mutant behaves typically as recombination less (recA) mutants of Escherichia coli and Pseudomonas aeruginosa, since as a recipient, it exhibits extremely low frequency of recombination following conjugational, transductional, and transformational gene transfer. Sex factor plasmids such as K-XYL or TOL can mobilize chromosomal genes equally well both from recA+ and recA801 donor cells, suggesting that host recombination functions are not necessary for mobilization of chromosomal genes by such plasmids.
D001426:Bacterial Proteins / Q000235:genetics*; D002876:Chromosomes, Bacterial; D003227:Conjugation, Genetic; D004265:DNA Helicases / Q000235:genetics; D010957:Plasmids; D011549:Pseudomonas / Q000235:genetics*; D011995:Recombination, Genetic; D014161:Transduction, Genetic; D014170:Transformation, Genetic
D001426:Bacterial Proteins; D004265:DNA Helicases