The mechanism of C-4 demethylation during cholesterol biosynthesis.
The conversion of 4,4-dimethylcholest-7-enol into 4alpha-methylcholest-7-enol by rat liver microsomal preparations involves the decarboxylation of a sterol 3-oxo-4alpha-carboxylic acid. By using an (18)O-labelled substrate it was shown that this decarboxylation does not involve a Schiff-base intermediate.
D000818:Animals; D002784:Cholesterol / Q000096:biosynthesis*; D003640:Dealkylation; D003653:Decarboxylation; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D009243:NAD; D009249:NADP; D051381:Rats; D012545:Schiff Bases
D012545:Schiff Bases; D009243:NAD; D009249:NADP; D002784:Cholesterol