Heme-associated subunit complex of cytochrome c oxidase identified by a new two-dimensional gel electrophoresis.
A Novel two-dimensional system (isoelectric focusing in the first dimension and sodium dodecyl sulfate-urea electrophoresis in the second) was used to determine the pI values of beef heart subunits of cytochrome c oxidase. The results identified a heme-associated complex of Subunits I, II, and IV. Conditions mitigate against migration of free heme.
D000818:Animals; D002417:Cattle; D003576:Electron Transport Complex IV; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006418:Heme / Q000032:analysis*; D007525:Isoelectric Focusing / Q000379:methods; D046911:Macromolecular Substances; D009206:Myocardium / Q000201:enzymology
D046911:Macromolecular Substances; D006418:Heme; D003576:Electron Transport Complex IV