Antigenic properties and molecular weights of murine leukemia virus-binding proteins.
A murine T lymphoma cell line, WEHI-22, has been studied for the presence of murine leukemia virus-binding proteins and for the presence of cell surface molecules that share antigens with mouse immunoglobulins. With surface radioiodination, detergent disruption, and immunoprecipitation, a 60 to 70,000-dalton molecule has been described that is recognized by chicken anti-mouse immunoglobulin serum. In competition experiments this molecule cross-reacts with highly purified mouse IgM myeloma proteins. A cell surface molecule of similar size can be shown to bind to mouse leukemia viruses. Pre-precipitation of the WEHI-22 cell surface material with chicken anti-mouse immunoglobulin removes the material binding to leukemia viruses.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D000954:Antigens, Surface; D000956:Antigens, Viral; D011232:Chemical Precipitation; D002645:Chickens; D003429:Cross Reactions; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D009052:Leukemia Virus, Murine / Q000276:immunology*; D008223:Lymphoma / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D008979:Moloney murine leukemia virus / Q000276:immunology; D011485:Protein Binding; D011817:Rabbits; D013154:Spleen / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D000954:Antigens, Surface; D000956:Antigens, Viral