title: [Comparative value of temperature marker and neutralization test for subtyping of herpes simplex viruses (author's transl)].
abstract: Two methods for the differentiation of herpes simplex virus (HSV)--rct and neutralization by specific antisera--have been applied to 8 prototype strains and 105 strains isolated from various clinical cases. The evaluation of the II/I index allows to choose the most specific rabbit antisera. The rct 40 degrees 3 C marker test gives comparable results for HSV grown in RK13 and human fibroblasts as well; it is in agreement with the immunological test in 48% of the cases. The delta tc values used to express the immunological typing allow to differentiate 76% of the strains (types 1 and 2). The others (24%) are equally neutralized by both specific antisera; they represent "intermediate" strains. The infection of man and animal by HSV (type 1, 2 or "intermediate") also produces heterospecific antibodies in variable amount. From clinical and immunological data rises the hypothesis that "intermediate" strains derive from HSV1 strains by antigenic variation during a persistent infection.
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D006561:Herpes Simplex / Q000276:immunology; D006358:Hot Temperature; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D009500:Neutralization Tests; D011817:Rabbits; D018139:Simplexvirus / Q000145:classification* / Q000276:immunology; D014779:Virus Replication
chemical_list: D007106:Immune Sera
vernacular_title: Valeur comparée du marquage thermique et de la neutralisation pour le typage des virus de l'herpès simplex.