A simple immunofluorescent technique for the detection of human rotavirus.
If trypsin is incorporated in the tissue culture medium it is possible to carry out a sensitive immunofluorescence assay for the presence of human rotavirus. The enhanced effect of trypsin is negated by serum. It has also been established that naturally occurring enzymes in faeces enable some virus to penetrate tissue culture cells. The role of these naturally occurring enzymes in the pathogenesis of rotavirus infection is discussed.
D000818:Animals; D002460:Cell Line; D002648:Child; D003470:Culture Media; D003937:Diagnosis, Differential; D005243:Feces / Q000382:microbiology; D005455:Fluorescent Antibody Technique; D005759:Gastroenteritis / Q000175:diagnosis; D000882:Haplorhini; D006801:Humans; D007668:Kidney; D008251:Macaca; D012328:RNA Viruses / Q000302:isolation & purification*; D012401:Rotavirus / Q000302:isolation & purification*; D014357:Trypsin / Q000494:pharmacology; D014777:Virus Diseases / Q000175:diagnosis
D003470:Culture Media; D014357:Trypsin