Rapid method for eluting IgG antibodies from erythrocytes coated with anti-Rh immune antibodies.
A method for eluting IgG antibodies from erythrocytes coated with anti-Rh immune antibodies has been developed, using temperature 56 degrees C as decomplexing factor and ion-exchange on DEAE Sephadex A-50 as antigen-binding factor.
D000918:Antibody Specificity; D003636:DEAE-Cellulose; D004912:Erythrocytes / Q000276:immunology*; D006801:Humans; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000302:isolation & purification*; D007474:Ion Exchange; D007518:Isoantibodies; D008722:Methods; D010641:Phenotype; D012204:Rh-Hr Blood-Group System; D013696:Temperature
D007074:Immunoglobulin G; D007518:Isoantibodies; D012204:Rh-Hr Blood-Group System; D003636:DEAE-Cellulose