Two different and compatible intraneuronal labels for ultrastructural study of synaptically related cells.
To examine the structural interactions between synaptically connected neurons in Aplysia, we have developed a method for simultaneously labeling two identified cell with different and compatible intraneuronal marking agents (horseradish peroxidase and [3H]N-acetyl-D-galactosamine) visible in both the light and electron microscopes. Combining these two agents within a single cell yields a third label.
D000116:Acetylgalactosamine; D000818:Animals; D001048:Aplysia; D001345:Autoradiography / Q000379:methods*; D005724:Ganglia / Q000033:anatomy & histology; D006735:Horseradish Peroxidase; D007395:Interneurons / Q000648:ultrastructure; D008465:Mechanoreceptors / Q000033:anatomy & histology; D009433:Neural Inhibition; D009474:Neurons / Q000648:ultrastructure; D013569:Synapses / Q000648:ultrastructure*; D009435:Synaptic Transmission; D014316:Tritium
D014316:Tritium; D006735:Horseradish Peroxidase; D000116:Acetylgalactosamine