A deoxyribonuclease of Diplococcus pneumoniae specific for methylated DNA.
A deoxyribonuclease specific for methylated DNA was isolated from Diplococcus pneumoniae. The enzyme, an endonuclease, degrades DNA for Escherichia coli to fragments of average molecular weight about half a million; it forms discrete fragments from phage lambda DNA. Methyl-deficient E. coli DNA is not attacked, neither is DNA from Micrococcus radiodurans, which contains no methylated adenine or cytosine. Nor is DNA from D. pneumoniae or phage T7 attacked. However, DNA from M. radiodurans, D. pneumoniae, and T7 is attacked after methylation with and E. coli extract. Methylated T7 DNA is degraded to discrete fragments. Although the genetic transforming activity of normal DNA from D. pneumoniae is not affected by the enzyme, transforming activity of methylated DNA is destroyed. The enzyme is designated endonuclease R Dpn I. Under certain conditions another enzyme of complementary specificity can be isolated. This enzyme, designated endonuclease R Dpn II, produces a similar pattern of fragments from the DNA of T7 without prior methylation of the DNA. It also degrades normal DNA for D. pneumoniae. It is suggested that this pair of enzymes plays a role in some unknown control process, which would involve a large fraction of the specific base sequences that are methylated in E. coli DNA and are present but not methylated in DNA from other sources.
D002850:Chromatography, Gel; D003090:Coliphages; D004267:DNA Viruses; D004269:DNA, Bacterial; D004279:DNA, Viral; D003851:Deoxyribonucleases / Q000302:isolation & purification / Q000378:metabolism*; D004586:Electrophoresis; D004591:Electrophoresis, Polyacrylamide Gel; D004720:Endonucleases / Q000302:isolation & purification / Q000378:metabolism*; D004926:Escherichia coli; D008745:Methylation; D012685:Sepharose; D013045:Species Specificity; D013296:Streptococcus pneumoniae / Q000201:enzymology*; D014170:Transformation, Genetic; D014783:Viscosity
D004269:DNA, Bacterial; D004279:DNA, Viral; D012685:Sepharose; D003851:Deoxyribonucleases; D004720:Endonucleases