[IMP-cyclohydrolase/transformylase from ehrlich-ascites carcinoma (author's transl)].
The IMP-cyclohydrolase/transformylase enzyme was purified from Ehrlich ascites tumor cells by ammonium sulfate fractionation and chromatography on Sephadex G-75 and DEAE-Sephadex. The electrophoretically pure enzyme has a molecular weight of about 350000, estimated by a gel filtration, and an isoelectric point of 6.2. It is composed of 8 subunits with a molecular weight of 46000. Every subunit is composed of two different proteins with a molecular weight of 18000 and 28500. Some further characteristics of the enzyme are reported.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000201:enzymology*; D005561:Formates; D006863:Hydrogen-Ion Concentration; D007093:Imidazoles; D007292:Inosine Nucleotides; D007525:Isoelectric Focusing; D007700:Kinetics; D046911:Macromolecular Substances; D051379:Mice; D008970:Molecular Weight; D009097:Multienzyme Complexes / Q000302:isolation & purification; D009709:Nucleotide Deaminases / Q000302:isolation & purification* / Q000378:metabolism; D010428:Pentosephosphates; D013696:Temperature; D014166:Transferases / Q000302:isolation & purification* / Q000378:metabolism
D005561:Formates; D007093:Imidazoles; D007292:Inosine Nucleotides; D046911:Macromolecular Substances; D009097:Multienzyme Complexes; D010428:Pentosephosphates; D014166:Transferases; D009709:Nucleotide Deaminases
Das IMP-Cyclohydrolase/Transformylase-Enzymsystem aus Ehrlich-Ascites-Tumorzellen