Neoplastic transformation of cultured Syrian hamster embryo cells by DNA of herpes simplex virus type 2.
Syrian hamster embryo cells were transformed to a neoplastic phenotype after exposure to herpes simplex virus type 2 (S-1) DNA at concentrations (less than or equal to 0.01 microgram per 60-mm dish) at which infectivity was no longer demonstrable. Transformed cells manifested in vitro phenotypic properties characteristic of the neoplastic state, expressed herpes simplex virus-specific antigens, and induced invasive tumors in vivo. Transfection and transformation of Syrian hamster embryo cells with herpes simplex virus type 2 DNA or its fragments is a suitable system for investigating the structure and function of herpes simplex virus-transforming gene(s).
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D002999:Clone Cells; D006224:Cricetinae; D004279:DNA, Viral; D008647:Mesocricetus; D009368:Neoplasm Transplantation; D010641:Phenotype; D018139:Simplexvirus / Q000235:genetics; D014162:Transfection
D004279:DNA, Viral