[DNA fragmentation of chicken adenovirus CELO by specific endonucleases R. HpaI, R. EcoRI, R. HindIII].
The effect of specific endonucleases on DNA chiken adenovirus CELO was studed. It was shown that endonucleases R. HpaI, R. EcoRI and R. HindIII cleaved viral DNA into 5,7 and 9 specific fragments, respectively. The sequence of fragments (physical map) was determined and found to be: D-A-E-C-B for enzyme R. HpaI; B-(EG)-C-A-D-F for enzyme R. EcoRI; H-F-A-C-G-B-D-E-I for enzyme R. HindIII.
D000256:Adenoviridae / Q000032:analysis*; D000259:Aviadenovirus / Q000032:analysis*; D001483:Base Sequence; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004279:DNA, Viral; D004926:Escherichia coli / Q000201:enzymology; D006190:Haemophilus / Q000201:enzymology; D009838:Oligodeoxyribonucleotides / Q000032:analysis; D013379:Substrate Specificity
D004279:DNA, Viral; D009838:Oligodeoxyribonucleotides; D004262:DNA Restriction Enzymes
Izuchenie fragmentatsii DNK ptich'ego adenovirusa CELO s pomoshch'iu spetsificheskikh endonukleaz R. HpaI, R. EcoRI, R. HindIII.