Co-fractionation of an endonuclease activity during the purification of DNA polymerase-alpha from regenerating rat liver. Properties and separation from DNA polymerase.
The presence of endonuclease activity associated with DNA polymerase was detected during the purification of high-molecular-weight DNA polymerase-alpha from regenerating rat liver by the use of a highly sensitive test. This endonuclease activity co-fractionated with DNA polymerase in a great variety of purification procedures involving ion-exchange chromatographies or molecular weight fractionation, but was further completely separated from DNA polymerase activity by using affinity chromatography on DNA-cellulose. The endonuclease acted on native or denatured DNA by introducing single-strand nicks in the DNA molecules; its enzymatic properties indicate that it could act in polymerisation conditions in vitro.
D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002845:Chromatography; D004254:DNA Nucleotidyltransferases / Q000302:isolation & purification*; D004279:DNA, Viral / Q000378:metabolism; D004720:Endonucleases / Q000302:isolation & purification / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006902:Hydroxymercuribenzoates / Q000494:pharmacology; D008099:Liver / Q000201:enzymology*; D008115:Liver Regeneration; D008274:Magnesium / Q000494:pharmacology; D008970:Molecular Weight; D051381:Rats; D013539:Simian virus 40; D012964:Sodium / Q000494:pharmacology
D004279:DNA, Viral; D006902:Hydroxymercuribenzoates; D012964:Sodium; D004254:DNA Nucleotidyltransferases; D004720:Endonucleases; D008274:Magnesium; D002118:Calcium