Raman and resonance-Raman scattering by erythrocyte ghosts.
1. We present the laser-Raman spectra of human erythrocyte ghosts, isolated by standard conditions and compare these with the spectra of lecithin liposomes and fat-free serum albumin. 2. The hydrocarbon stretching modes of membrane lipids are temperature sensitive and may serve as a index of hydrocarbon chain motion. 3. The Amide I and Amide III bands of ghosts in H-2O and 2-H-2O, indicate a mixture of alpha-helical and unordered conformation, but do not allow a quantitative estimate of secondary structure. 4. Strong, scattering bands at 1530 and 1165 cm-1 are attributable to conjugated double bond systems, probably of membrane-associated carotenoids. Their high intensity is due to resonance enhancement.
D000818:Animals; D002417:Cattle; D002462:Cell Membrane / Q000648:ultrastructure*; D002794:Choline / Q000032:analysis; D004530:Egg Yolk; D004912:Erythrocytes / Q000648:ultrastructure*; D005260:Female; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007834:Lasers; D008081:Liposomes; D008968:Molecular Conformation; D010713:Phosphatidylcholines; D011487:Protein Conformation; D012542:Scattering, Radiation; D012710:Serum Albumin, Bovine; D013050:Spectrometry, Fluorescence; D013057:Spectrum Analysis
D008081:Liposomes; D010713:Phosphatidylcholines; D012710:Serum Albumin, Bovine; D002794:Choline