Effect of pH, temperature and incubation period on the study of intrinsic innervation of various organs by thiocholine technique in certain vertebrates.
The adrenergic fibres can be demonstrated if the optimal time and temperature are maintained. The cholinergic fibres were demonstrated at pH 5.2, incubation period l4h and temperature 37 degrees C. To ascertain the cholinesterase activity in tongue, heart, lung(birds, mammals and reptiles), gizzard and proventriculus (birds) and penis (rat and embryo of squirrel), pH 4.9, 16h incubation and temperature 37 degrees C, were quite suitable. It was possible to demonstrate the intrinsic innervation in tongue, lung, heart (mammals, birds and reptiles), gizzard and proventriculus (birds) and penis (rat and embryo of squirrel), at pH 5.2, 20h incubation and temperature 40 degrees C.
D000818:Animals; D004386:Duodenum / Q000294:innervation; D005260:Female; D006321:Heart / Q000294:innervation; D006651:Histocytochemistry; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007421:Intestine, Small / Q000294:innervation; D008168:Lung / Q000294:innervation; D008297:Male; D010179:Pancreas / Q000294:innervation; D010275:Parasympathetic Nervous System / Q000378:metabolism / Q000502:physiology*; D010413:Penis / Q000294:innervation; D010525:Peripheral Nerves / Q000502:physiology; D013045:Species Specificity; D013270:Stomach / Q000294:innervation; D013564:Sympathetic Nervous System / Q000378:metabolism / Q000502:physiology*; D013696:Temperature; D013860:Thiocholine / Q000378:metabolism; D013997:Time Factors; D014059:Tongue / Q000294:innervation; D014714:Vertebrates
D013860:Thiocholine