[Quantitative determination of IgA from human bronchial mucus (author's transl)].
Before quantitative analysis of bronchial mucus proteins, sputum must be transformed into a homogeneous medium: this is done by ultrasonic action and contact with cationic resin AG 50 W X 2. Then, acid mucins are fixed on Ecteola-cellulose, and proteins extracted by a phosphate/saline buffer at pH 5. IgA is estimated by electroimmunodiffusion. Reproducibility of the method is discussed: the accuracy for IgA values is 4%.
D000818:Animals; D001980:Bronchi / Q000032:analysis*; D002482:Cellulose; D002852:Chromatography, Ion Exchange; D005069:Evaluation Studies as Topic; D006863:Hydrogen-Ion Concentration; D005779:Immunodiffusion; D007070:Immunoglobulin A / Q000032:analysis*; D008722:Methods; D009093:Mucus / Q000032:analysis*; D014465:Ultrasonics
D007070:Immunoglobulin A; D002482:Cellulose
Determination quantitative des IgA du mucus bronchique humain