Cloning of the active thymidine kinase gene of herpes simplex virus type 1 in Escherichia coli K-12.
A herpes simplex virus DNA fragment that is produced by digestion with BamHI endonuclease and carries the thymidine kinase (TK; ATP:thymidine 5'-phosphotransferase, EC 2.7.1.21) gene has been cloned in Escherichia coli. A recombinat plasmid, pFG5, has been analyzed extensively and a detailed restriction map is presented. pFG5 DNA efficiently transforms TK- mouse L cells. The TK coding sequence in the cloned fragment has been localized and a smaller recombinant plasmid, pAG0, also carrying an active TK gene, has been constructed to serve as a more convenient vector for transfer, into TK- cells, of genes previously cloned in E. coli.
D000937:Antigen-Antibody Reactions; D002472:Cell Transformation, Viral; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D010957:Plasmids; D018139:Simplexvirus / Q000201:enzymology / Q000235:genetics*; D013937:Thymidine Kinase / Q000235:genetics* / Q000276:immunology
D004274:DNA, Recombinant; D013937:Thymidine Kinase; D004262:DNA Restriction Enzymes