Phosphopyridoxal complexes with histamine and histidine. (2) The influence of presumed complex on histidine decarboxylase activity in rat gastric mucosa.
It was found that in vitro, histidine and histamine form a complex compound with pyridoxal-5'-phosphate (PLP). The purpose of the present experiments was to find whether formation of this complex can influence histidine decarboxylase activity. It was found that excess PLP inhibits rat's gastric mucosa histidine decarboxylase activity in vitro. The inhibitory action of PLP depends on the histidine concentration and is independent on the amount of crude enzyme preparation. The histidine-PLP complex did not influence enzyme activity. The possible mechanisms of the inhibitory action of PLP on histidine decarboxylase activity are discussed.
D000818:Animals; D002262:Carboxy-Lyases / Q000378:metabolism*; D005753:Gastric Mucosa / Q000187:drug effects / Q000201:enzymology*; D006632:Histamine / Q000494:pharmacology*; D006639:Histidine / Q000494:pharmacology*; D006640:Histidine Decarboxylase / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007700:Kinetics; D009994:Osmolar Concentration; D011732:Pyridoxal Phosphate / Q000494:pharmacology*; D051381:Rats
D006639:Histidine; D011732:Pyridoxal Phosphate; D006632:Histamine; D002262:Carboxy-Lyases; D006640:Histidine Decarboxylase