Identification of transcribed regions of Epstein-Barr virus DNA in Burkitt lymphoma-derived cells.
RNA was extracted from the Burkitt lymphoma-derived cell line Raji and from Burkitt lymphoma tumor biopsies, isotope labeled in vitro by iodination with 125I, and hybridized to electrophoretically separated restriction endonuclease fragments of Epstein-Barr virus DNA on nitrocellulose membranes. The results indicated that only certain parts of the Epstein-Barr virus genome are represented as polyribosomal RNA in Raji cells, with a pronounced dominance of RNA sequences complementary to a 2.0 x 10(6)-dalton segment of Epstein-Barr virus DNA located close to the left end of the viral genome. A map of virus-specific polyribosomal RNA sequences was constructed, which indicated that a minimum of three regions of the Epstein-Barr virus genome are expressed in Raji cells. Total-cell RNA preparations from five Burkitt lymphoma biopsies contained RNA sequences homologous to the same regions of Epstein-Barr virus DNA as polyribosomal RNA from Raji cells, albeit at different relative proportions.
D001483:Base Sequence; D002051:Burkitt Lymphoma / Q000032:analysis*; D002460:Cell Line; D004279:DNA, Viral / Q000032:analysis; D004854:Herpesvirus 4, Human / Q000032:analysis*; D006801:Humans; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D012334:RNA, Neoplasm / Q000032:analysis*; D012335:RNA, Ribosomal / Q000032:analysis*; D012367:RNA, Viral / Q000032:analysis*
D004279:DNA, Viral; D012334:RNA, Neoplasm; D012335:RNA, Ribosomal; D012367:RNA, Viral