Studies on the mechanism of macrophage aggregation induced by lymphokines.
Macrophage aggregation (measured by a light-scattering technique) induced by unpurified lymphokine preparations has been investigated. This has been shown to be Ca2+ or Mg2+ dependent and to be inhibited by a number of agents known to affect cellular physiology and biochemistry. The divalent cation ionophore A23187 causes a macrophage aggregation superficially similar to that induced by lymphokines, although differences were found. It is suggested that a biochemical response by the macrophage is required for aggregation induced by lymphokines, and thus measurements of macrophage aggregation allow a study of the early events in the response of macrophages to lymphokines.
D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D002449:Cell Aggregation / Q000187:drug effects; D000242:Cyclic AMP / Q000378:metabolism; D004492:Edetic Acid / Q000494:pharmacology; D006019:Glycolysis / Q000187:drug effects; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008222:Lymphokines / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D008274:Magnesium / Q000494:pharmacology
D008222:Lymphokines; D000001:Calcimycin; D004492:Edetic Acid; D000242:Cyclic AMP; D008274:Magnesium; D002118:Calcium