Rapid determination of selenium in various substrates by electron capture gas-liquid chromatography.
In the proposed procedure for the determination of selenium, 0.5-1 g of sample is wet ashed with concentrated nitric acid. After adding 1,2-diamino-4,5-dichlorobenzene to the digest at pH 1, the resulting dichloropiazselenol derivatives is extracted with toluene. The extract is purified by column chromatography over Florisil and analyzed by gas-liquid chromatography with electron capture detection. Recoveries of selenium added to various substrates ranged from 72 to 102%. The limit of detection is approximately 0.01 ppm, but smaller amounts can be determined by increasing the sample size or by concentration of the final extract.
D002722:Chlorobenzenes; D002845:Chromatography / Q000379:methods*; D002849:Chromatography, Gas / Q000379:methods; D002855:Chromatography, Thin Layer; D003959:Diamines; D004531:Eggs / Q000032:analysis; D004583:Electrons; D006863:Hydrogen-Ion Concentration; D010944:Plants / Q000032:analysis; D012643:Selenium / Q000032:analysis*
D002722:Chlorobenzenes; D003959:Diamines; D012643:Selenium