Characterization of the deoxyribonucleic acid polymerase associated with Kilham rat virus.
Purified preparations of the parvovirus, Kilham rat virus, have associated with them a protein with DNA polymerase activity. The enzyme has been separated from the other two or three viral proteins and purified 63-fold. The viral associated enzyme was found in a single peak of DNA polymerase activity after chromatography on DEAE-cellulose, DNA-cellulose, and phosphocellulose columns. It shares some properties in common with the host cellular DNA polymerases, described in the preceding paper (Salzman, L.A., and McKerlie, L. (1975) J. Biol. Chem. 250, 5589-5595), but also has some important distinguishing characteristics. The Kilham rat virus-associated DNA polymerase has increased enzyme activity in the presence of 0.02 M KCl and has a strong preference for a synthetic DNA polymer containing deoxyadenylate and deoxythymidylate. The enzyme has a molecular weight of approximately 75,000 plus or minus 3,000 and appears to contain endonuclease activity.
D000818:Animals; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D002729:Chloromercuribenzoates / Q000494:pharmacology; D002846:Chromatography, Affinity; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D004254:DNA Nucleotidyltransferases / Q000378:metabolism*; D004279:DNA, Viral / Q000096:biosynthesis; D011756:Diphosphates / Q000494:pharmacology; D004996:Ethidium / Q000494:pharmacology; D005092:Exonucleases / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D008970:Molecular Weight; D009711:Nucleotides / Q000378:metabolism; D010321:Parvoviridae / Q000201:enzymology*; D011189:Potassium Chloride / Q000494:pharmacology; D051381:Rats; D013539:Simian virus 40; D012965:Sodium Chloride / Q000494:pharmacology; D013698:Templates, Genetic; D014316:Tritium; D009396:Wilms Tumor / Q000201:enzymology*
D002729:Chloromercuribenzoates; D004279:DNA, Viral; D011756:Diphosphates; D009711:Nucleotides; D014316:Tritium; D008345:Manganese; D012965:Sodium Chloride; D011189:Potassium Chloride; D004254:DNA Nucleotidyltransferases; D005092:Exonucleases; D004996:Ethidium; D008274:Magnesium