title: Renin measurement in blood collected from the efferent arteriole of the kidney of the rat.
abstract: Plasma renin could be detected in 16 nl of rat plasma by incubating at 50 degrees C, pH 6.2 with 20 mul of a rat renin substrate. In 60 nl samples it could be estimated and differences in concentration of 20% could be detected. Plasma renin concentration was measured in blood from the superficial efferent arterioles of a rat's kidney. The concentration of renin in different efferent arterioles of the same rat were similar. The renin concentration of efferent arteriolar blood was 361 plus or (-1)89 (S.D.) ng Al ml minus 1 (blood) hour (-1) which was not different from the renin concentration of simultaneously taken femoral artery blood (340 +/- 217). Plasma concentration can be measured in efferent arteriolar blood and will allow the control of renin secretion to be studied.
mesh_terms: D000804:Angiotensin II / Q000032:analysis; D000818:Animals; D001158:Arteries; D001769:Blood; D005246:Feedback; D005263:Femoral Artery; D006863:Hydrogen-Ion Concentration; D007678:Kidney Glomerulus / Q000098:blood supply*; D051381:Rats; D012083:Renin / Q000097:blood*
chemical_list: D000804:Angiotensin II; D012083:Renin