Large-scale isolation of equine liver alcohol dehydrogenase on a blue agarose gel.
Equine liver alcohol dehydrogenase (EC 1.1.1.1) has been purified by a new scheme using a blue agarose gel (Blue Sepharose) as an affinity sorbent. Starting amounts of 0.6 to 10 kg liver have been processed to enzyme possessing 1.5 U/mg average specific activity, in about three to four days. Some parameters concernining adsorption of enzyme to the blue gel as well as recovery therefrom have been explored.
D000429:Alcohol Oxidoreductases / Q000302:isolation & purification* / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D002847:Chromatography, Agarose; D002848:Chromatography, DEAE-Cellulose; D004591:Electrophoresis, Polyacrylamide Gel; D005782:Gels; D006736:Horses; D008099:Liver / Q000201:enzymology*; D009243:NAD / Q000378:metabolism; D011134:Polysaccharides; D012685:Sepharose; D012967:Sodium Dodecyl Sulfate
D005782:Gels; D011134:Polysaccharides; D009243:NAD; D012967:Sodium Dodecyl Sulfate; D012685:Sepharose; D000429:Alcohol Oxidoreductases