title: Regional localization of the gene for human phosphoribosylpyrophosphate synthetase on the X chromosome.
abstract: Sixty-eight independent hybrid clones were isolated after irradiated normal human lymphocytes were fused with Chinese hamster fibroblasts lacking hypoxanthine-guanine phosphoribosyltransferase activity. The cells were grown under selective conditions requiring retention of the X chromosome-linked locus for human hypoxanthine-guanine phosphoribosyltransferase. The frequency and patterns of cotransference of human phosphoribosylpyrophosphate synthetase with the selected marker and with additional X-linked enzymatic markers confirm X linkage of the structural gene for human phosphoribosylpyrophosphate synthetase and support assignment of this gene to a position on the long arm of the X, between the loci for alpha-galactosidase and hypoxanthine-guanine phosphoribosyltransferase.
mesh_terms: D002874:Chromosome Mapping; D005260:Female; D008040:Genetic Linkage; D006801:Humans; D006822:Hybrid Cells; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics; D010770:Phosphotransferases / Q000235:genetics*; D012268:Ribose-Phosphate Pyrophosphokinase / Q000235:genetics*; D012730:Sex Chromosomes; D014960:X Chromosome
chemical_list: D007041:Hypoxanthine Phosphoribosyltransferase; D010770:Phosphotransferases; D012268:Ribose-Phosphate Pyrophosphokinase