Kinetic studies of the blood serum xanthine dehydrogenase inhibition by alloxan (2,4,5,6-tetraoxypyrimidine 5,6-dioxyuracil).
Alloxan is an inhibitor of the enzyme xanthine: NAD+ oxido reductase (E.C.1.2.1.37). Alloxan acts as an electron acceptor and competes "in vitro" with the tetrazolium salt used as electron acceptor in the assay system used for the determination of the dehydrogenase activity of the enzyme. The alloxan inhibition was reverted when phenazine methosulfate (PMS) was added to the system.
D000496:Alloxan / Q000494:pharmacology*; D004579:Electron Transport; D066298:In Vitro Techniques; D007658:Ketone Oxidoreductases / Q000037:antagonists & inhibitors*; D007700:Kinetics; D009243:NAD / Q000494:pharmacology; D010619:Phenazines / Q000494:pharmacology; D013778:Tetrazolium Salts / Q000494:pharmacology*; D014968:Xanthine Dehydrogenase / Q000037:antagonists & inhibitors* / Q000097:blood / Q000494:pharmacology
D010619:Phenazines; D013778:Tetrazolium Salts; D009243:NAD; D000496:Alloxan; D014968:Xanthine Dehydrogenase; D007658:Ketone Oxidoreductases