Incorporation of L-[1-14C]leucine into protein by liver postmitochondrial supernatant: opposing effects of preincubated nicotinamide-adenine dinucleotide phosphate and 4-dimethylamino-3'-methylazobenzene.
Combination of preincubated drug-metabolizing medium containing NADP+ with a cell-free protein-synthesizing system resulted in marked stimulation of incorporation of L-[1-14C]leucine into protein. Addition of 4-dimethylamino-3'-methylazobenzene, present and previously preincubated in the drug-metabolizing medium, decreased this effect.
D000818:Animals; D007930:Leucine / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D009249:NADP / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D014176:Protein Biosynthesis; D051381:Rats; D004124:p-Dimethylaminoazobenzene / Q000494:pharmacology*
D009249:NADP; D004124:p-Dimethylaminoazobenzene; D007930:Leucine