Responses of immune and nonimmune adult rhesus macaques (Macaca mulatta) to adenovirus SV-20.
Adenovirus SV-20 (ASV-20) was inoculated subcutaneously into adult rhesus macaques (Macaca mulatta), and various immunologic parameters were studied. Similar changes were observed in macaques that had anti-ASV-20 serum-neutralizing antibodies prior to inoculation and in those lacking detectable antibodies. There were absolute decreases in numbers of peripheral blood lymphocytes (PBL), erythrocyte-rosetting lymphocytes, complement-receptor lymphocytes, and Fc-receptor lymphocytes. These changes were most significant (P less than 0.05) on postinoculation days (PID) 3 and 7. Mitogenic responsiveness to concanavalin A, phytohemagglutinin, and pokeweed mitogen in cultured PBL from immune and nonimmune macaques was depressed on PID 3, 7, and 14. Ultraviolet-inactivated ASV-20 caused moderate suppression of phytohemagglutinin-induced mitogenesis when viral particles and lectin were added simultaneously to PBL cultures. Plasma cortisol (hydrocortisone) values were not significantly altered following inoculation of ASV-20. High titers of anti-ASV-20 antibody developed by PID 7 in nonimmune macaques, and previously immune macaques showed a booster effect in the same time period. Antibody titers were still increased 120 days after inoculation. There was no clinical evidence of an adverse effect of ASV-20 infection in these macaques.
D000256:Adenoviridae / Q000276:immunology* / Q000472:pathogenicity; D000257:Adenoviridae Infections / Q000276:immunology / Q000662:veterinary; D000261:Adenoviruses, Simian / Q000276:immunology*; D000818:Animals; D000917:Antibody Formation; D005260:Female; D000882:Haplorhini; D007109:Immunity; D007111:Immunity, Cellular; D007958:Leukocyte Count; D008214:Lymphocytes / Q000276:immunology; D008253:Macaca mulatta / Q000276:immunology; D008297:Male; D008992:Monkey Diseases / Q000276:immunology; D010835:Phytohemagglutinins / Q000494:pharmacology
D010835:Phytohemagglutinins