Transcriptional regulation of the yeast cytochrome c gene.
DNA from the cloned yeast iso-1-cytochrome c, cycl, gene was used in a hybridization assay to measure levels and rates of synthesis of cycl RNA. Derepressed cells synthesized cycl RNA at 6 times the rate of that of glucose-repressed cells. Upon glucose addition to a derepressed culture, the transcription of the cycl gene was repressed within 2.5 min. The half-life of hybridizable cycl RNA was determined to be 12-13.5 min under repressed and derepressed conditions and during repression. The results demonstrate that the expression of the cycl gene is subject to transcriptional regulation.
D003574:Cytochrome c Group / Q000235:genetics*; D004274:DNA, Recombinant; D005796:Genes; D005947:Glucose / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000378:metabolism; D011887:Raffinose / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014158:Transcription, Genetic
D003574:Cytochrome c Group; D004274:DNA, Recombinant; D012333:RNA, Messenger; D005947:Glucose; D011887:Raffinose