Hydrolytic and transglucosylation activities of a purified calf spleen beta-glucosidase.
Certain properties of the transglucosylitic activity and the hydrolytic activity of a purified calf spleen beta-glucosidase (beta-D-glucoside glucohydrolase EC 3.2.1.21) were investigated. There was a stimulation of both activities by sodium taurocholate and "Gaucher's factor". The K-m values for 4-methylumbelliferyl-beta-D-glucoside and glucosylceramide as donors in the transglucosylation reaction were 2 mM and 0.075 mM, respectively. The K-m for ceramide as acceptor was 0.149 mM with both of these compounds. The ability of several glucoside to act as donors was examined. The capacity to catalyze this "transglucosylation" reaction is greatly diminished in spleen tissue samples from Gaucher's patients. The enzyme possesses the capacity to hyrolyze 4-methylumbellifery-beta-D-glucoside, p-nitrophenyl-beta-D-glucoside, glucosylsphingosine, glucosylceramide and deoxycorticosterol-beta-D-glucoside. It is postulated that a single enzyme protein may be responsible for both the hydrolytic and the transglucosylitic activities.
D000818:Animals; D002417:Cattle; D002518:Ceramides; D004789:Enzyme Activation / Q000187:drug effects; D005776:Gaucher Disease / Q000503:physiopathology; D005959:Glucosidases / Q000378:metabolism*; D005964:Glucosyltransferases / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006923:Hymecromone; D007700:Kinetics; D009596:Nitrophenols; D013110:Sphingosine; D013154:Spleen / Q000201:enzymology* / Q000503:physiopathology; D013656:Taurocholic Acid / Q000494:pharmacology
D002518:Ceramides; D009596:Nitrophenols; D006923:Hymecromone; D013656:Taurocholic Acid; D005964:Glucosyltransferases; D005959:Glucosidases; D013110:Sphingosine