Inhibition of Hageman factor, plasma thromboplastin antecedent, thrombin and other clotting factors by phenylglyoxal hydrate (38500).
Exposure of purified Hageman factor (HF, Factor XII) to phenylglyoxal hydrate (PHG), an agent reacting with arginine residues in protein, inhibited its coagulant properties upon subsequent exposure of negatively charged agents. Once HF had been exposed to kaolin or ellagic acid, however, subsequent addition of PHG was much less inhibitory. PHG had no effect upon the ability of HF to bind to negatively charged surfaces. PGH also inhibited preparations of activated PTA (Factor XI) and thrombin, and, when incubated with plasma, reduced the titer of coagulable fibrinogen, PTA Christmas factor (Factor IX), antihemophilic factor (Factor VIII), Factor VII, Stuart factor (Factor X), proaccelerin (Factor V) and prothrombin (Factor II), and to a lesser degres, HF.
D000447:Aldehydes / Q000031:analogs & derivatives* / Q000494:pharmacology*; D001578:Benzopyrans / Q000494:pharmacology; D001777:Blood Coagulation / Q000187:drug effects*; D001779:Blood Coagulation Factors / Q000037:antagonists & inhibitors*; D005164:Factor IX / Q000037:antagonists & inhibitors; D005165:Factor V / Q000037:antagonists & inhibitors; D005167:Factor VII / Q000037:antagonists & inhibitors; D005169:Factor VIII / Q000037:antagonists & inhibitors; D005170:Factor X / Q000037:antagonists & inhibitors; D005172:Factor XI / Q000037:antagonists & inhibitors*; D005174:Factor XII / Q000037:antagonists & inhibitors*; D006037:Glyoxal / Q000031:analogs & derivatives* / Q000494:pharmacology*; D006490:Hemostatics; D007616:Kaolin / Q000494:pharmacology; D011516:Prothrombin / Q000037:antagonists & inhibitors; D011517:Prothrombin Time; D013917:Thrombin / Q000037:antagonists & inhibitors*
D000447:Aldehydes; D001578:Benzopyrans; D001779:Blood Coagulation Factors; D006490:Hemostatics; D007616:Kaolin; D006037:Glyoxal; D005165:Factor V; D005167:Factor VII; D011516:Prothrombin; D005169:Factor VIII; D005164:Factor IX; D005170:Factor X; D005174:Factor XII; D005172:Factor XI; D013917:Thrombin