[Influence of glutaraldehyde on Na+, K+-ATPase activity of brain tissue and enzymic preparations under conditions of electron cytochemical analysis].
The glutaraldehyde inhibitory influence on Mg2+, Na+, K+-ATPase activity of the brain tissue is shown to increase with a rise in the glutaraldehyde concentration; a direct dependence is established between the degree of inhibition and the treatment time and temperature. The glutaraldehyde inhibitory effect on the activity of the brain microsomal fraction Na+, K+-ATPase preparations under the same treatment conditions is considerably higher. A histochemical detection of the cerebral cortex Mg2+, Na+, K+-ATPase was carried out under optimal conditions of fixation, and a conclusion is made on possibility of using glutaraldehyde as a fixator in histochemical investigations.
D000447:Aldehydes / Q000494:pharmacology*; D000818:Animals; D001921:Brain / Q000201:enzymology* / Q000648:ultrastructure; D005976:Glutaral / Q000494:pharmacology*; D006651:Histocytochemistry; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D008854:Microscopy, Electron; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
D000447:Aldehydes; D000254:Sodium-Potassium-Exchanging ATPase; D008274:Magnesium; D005976:Glutaral
Vliianie glutaral'degida na Na+, K+-ATP-aznuiu aktivnost' tkani i Fermentnykh preparatov mozga v usloviiakh provedeniia elektronnotsitokhimicheskogo analiza.