The metabolism of N-ethyl-N-methylaniline by rabbit liver microsomes: the measurement of metabolites by gas-liquid chromatography.
1. A method for the determination of N-ethyl-N-methylaniline and its metabolites by g.l.c. is described. 2. Following incubation in N-ethyl-N-methylaniline with rabbit liver microsomes for 60 min, over 95% of the substrate was accounted for as unchanged compound or metabolites. 3. N-Ethyl-N-methylaniline is metabolized in vitro by rabbit tissues mainly by N-oxidation and N-demethylation and to a lesser extent by N-deethylation and di-dealkylation. 4. Both major routes of metabolism were observed in homogenates prepared from rabbit liver and lung; in addition N-oxidation occurred in kidney and bladder tissue homogenates.
D000814:Aniline Compounds / Q000378:metabolism*; D000818:Animals; D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D006358:Hot Temperature; D008099:Liver / Q000378:metabolism; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D009249:NADP / Q000494:pharmacology; D009928:Organ Specificity; D010089:Oxidoreductases, N-Demethylating / Q000378:metabolism; D011817:Rabbits; D013329:Structure-Activity Relationship; D013347:Subcellular Fractions / Q000378:metabolism
D000814:Aniline Compounds; D009249:NADP; C010951:N-ethyl-N-methylaniline; D010089:Oxidoreductases, N-Demethylating