SV40 DNA injected into Xenopus oocyte nuclei is transcribed by RNA polymerase B.
SV40 DNA I. injected into Xenopus oocyte nuclei is transcribed. The SV40-specific RNA molecules migrate on sucrose gradients as do viral RNAs formed in infected green monkey cells but a variable proportion of RNA sequences complementary to SV40 DNA is also found in the light region of the gradients. All SV40-specific RNA species seem to be synthesized by RNA polymerase B as their synthesis is completely sensitive to low concentrations (0.1 microgram/ml) of alpha-amanitin. Concomittantly, the formation of SV40-specific proteins (tumor antigens) is inhibited by injecting alpha-amanitin together with the SV40 DNA.
D000546:Amanitins / Q000494:pharmacology; D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D003584:Cytological Techniques; D004279:DNA, Viral / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D005260:Female; D008845:Microinjections; D009865:Oocytes / Q000378:metabolism*; D010063:Ovum / Q000378:metabolism*; D012319:RNA Polymerase II / Q000378:metabolism*; D012367:RNA, Viral / Q000096:biosynthesis; D013539:Simian virus 40; D014158:Transcription, Genetic; D014981:Xenopus
D000546:Amanitins; D004279:DNA, Viral; D012367:RNA, Viral; D012319:RNA Polymerase II; D012321:DNA-Directed RNA Polymerases