Preparation and properties of beta-casein from buffalo's milk.
Beta-casein from individual buffalo's milk was found to be homogeneous by starch-gel electrophoresis. Beta-Casein was separated from buffalo's milk by the method of Warner (1944) and purified by DEAE-cellulose chromatography. Buffalo beta-casein possesses identical end-groups to those of cow beta-casein; namely N-terminal arginine and assuming a single polypeptide chain a possible C-terminal sequence of Ile-Ile-Val. However, the amino-acid composition and the trypic peptide patterns of the 2 proteins are not the same.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002020:Buffaloes / Q000378:metabolism*; D002268:Carboxypeptidases; D002364:Caseins / Q000032:analysis / Q000302:isolation & purification*; D002417:Cattle; D005260:Female; D006863:Hydrogen-Ion Concentration; D008892:Milk / Q000032:analysis*; D010446:Peptide Fragments / Q000032:analysis; D011247:Pregnancy; D013045:Species Specificity; D014357:Trypsin
D000596:Amino Acids; D002364:Caseins; D010446:Peptide Fragments; D002268:Carboxypeptidases; D014357:Trypsin