Pattern generation in the lobster (Panulirus) stomatogastric ganglion. II. Pyloric network simulation.
1. Results from the companion paper were incorporated into a physiologically realistic computer model of the three principal cell types (PD/AB, LP, PY) of the pyloric network in the stomatogastric ganglion. Parameters for the model were mostly calculated (sometimes estimated) from experimental data rather than fitting the model to observed output patterns. 2. The initial run was successful in predicting several features of the pyloric pattern: the observed gap between PD and LP bursts, the appropriate sequence of the activity periods (PD, LP, PY), and a substantial PY burst not properly simulated by an earlier model. 3. The major discrepancy between model and observed patterns was the too-early occurrence of the PY burst, which resulted in a much shortened LP burst. Motivated by this discrepancy, additional investigations were made of PY properties. A hyperpolarization-enabled depolarization-activated hyperpolarizing conductance change was discovered which may make an important contribution to the late phase of PY activity in the normal burst cycle. Addition of this effect to the model brought its predictions more in line with observed patterns. 4. Other discrepancies between model and observation were instructive and are discussed. The findings force a substantial revision in previously held ideas on pattern production in the pyloric system. More weight must be given to functional properties of individual neurons and less to properties arising purely from network interactions. This shift in emphasis may be necessary in more complicated systems as well. 5. An example has been provided of the value quantitative modeling can be to network physiology. Only through rigorous quantitative testing can qualitative theories of how the nervous system operates be substantiated.
D000818:Animals; D004558:Electric Stimulation; D005724:Ganglia / Q000502:physiology*; D008564:Membrane Potentials; D008959:Models, Neurological; D008121:Nephropidae; D009415:Nerve Net / Q000502:physiology*; D009424:Nervous System Physiological Phenomena; D009474:Neurons / Q000502:physiology*; D011708:Pylorus / Q000294:innervation*; D013270:Stomach / Q000294:innervation; D013569:Synapses / Q000502:physiology; D009435:Synaptic Transmission; D013997:Time Factors