title: Radioactive labeling of proteins with an iodinated amidination reagent.
abstract: An iodinated phenolic imidoester has been synthesized for the labeling of proteins to high specific activities with radioactive iodine. The main advantage of this two-step labeling method is that it obviates direct contact of the protein with deleterious oxidizing agents, such as chloramine-T, pressent in direct methods of iodinating proteins.
mesh_terms: D000818:Animals; D001555:Benzene Derivatives / Q000138:chemical synthesis; D004285:Dogs; D006863:Hydrogen-Ion Concentration; D007094:Imides / Q000138:chemical synthesis; D007202:Indicators and Reagents; D007457:Iodine Radioisotopes; D007553:Isotope Labeling / Q000379:methods*; D011506:Proteins; D012709:Serum Albumin; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature
chemical_list: D001555:Benzene Derivatives; D007094:Imides; D007202:Indicators and Reagents; D007457:Iodine Radioisotopes; D011506:Proteins; D012709:Serum Albumin