Production of antiviral and migration inhibitory activities in the peritoneum of mice after inoculation with allogenic Ehrlich ascites tumor cells.
Peritoneal inoculation of Ehrlich ascites cells into mice stimulates the appearance of migration inhibitory factor (MIF) not only in the peritoneum but also in the circulation of mice. Whereas the MIF-activity in the peritoneum reached maximum at 6 days and disappeared within 17 days, the MIF-activity of sera reached maximum levels at about 11 days after intraperitoneal inoculation of tumor cells (10(6) of EAC per mouse). In addition, an antiviral activity of peritoneal exudates was observed in the early stage (24--72 h) of tumor challenge. The time-course of appearance of both MIF and AV-inhibitor are presented. A significant decrease of MIF-activity was noted when the tumor-inoculated mice were treated with ds-RNA, antithymocyte serum and cyclophosphamide, respectively. The appearance of antiviral activity in the peritoneum of tumor-inoculated mice seems to be macrophage-dependent. Although the antiviral factor meets several criteria of interferons, further studies are required for its characterization.
D000818:Animals; D000998:Antiviral Agents; D001202:Ascitic Fluid / Q000166:cytology / Q000502:physiology*; D002286:Carcinoma, Ehrlich Tumor / Q000503:physiopathology*; D002455:Cell Division; D003520:Cyclophosphamide / Q000494:pharmacology; D004686:Encephalomyocarditis virus; D007106:Immune Sera; D007700:Kinetics; D007739:L Cells / Q000502:physiology; D008263:Macrophage Migration-Inhibitory Factors / Q000502:physiology*; D051379:Mice; D012313:RNA / Q000494:pharmacology; D013950:Thymus Gland / Q000276:immunology
D000998:Antiviral Agents; D007106:Immune Sera; D008263:Macrophage Migration-Inhibitory Factors; D012313:RNA; D003520:Cyclophosphamide