Spectroscopic forms of carbonmonoxi-cytochrome oxidase.
A systematic study of the errors of low-temperature recording of kinetics of the cytochrome oxidase-CO reaction had identified the classic devitrification process of Keilin & Hartree [(1950) Nature (London)165, 504-505]. The methodology described here minimizes this effect, and the computation methods afford appropriate ways of detecting a residual effect. Thus it has been possible to identify that absorption difference spectra and kinetics of the reaction of fully reduced or half-reduced cytochrome oxidase with CO indicate only one spectroscopic form of the respective carbonmonoxi-cytochrome oxidase.
D000818:Animals; D002248:Carbon Monoxide; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D003576:Electron Transport Complex IV; D005615:Freezing; D007700:Kinetics; D008722:Methods; D013053:Spectrophotometry
D002248:Carbon Monoxide; D003576:Electron Transport Complex IV