[Cryopreservation of hemopoietic stem cells (author's transl)].
The CFU-S potential of mouse bone marrow, the transplantation capacity of autologous and allogeneic monkey bone marrow and the CFU-C potential of human bone marrow can be cryopreserved without loss of viability. It is critical for an optimal stem cell recovery to avoid an osmotic shock after thawing. The intracellular cryoprotectives glycerol and DMSO which provide a high osmotic pressure should be removed from the thawed cells by a slow stepwise dilution procedure.
D000818:Animals; D002470:Cell Survival; D003451:Cryoprotective Agents; D004121:Dimethyl Sulfoxide; D005615:Freezing; D005990:Glycerol; D000882:Haplorhini; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells; D006801:Humans; D008253:Macaca mulatta; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009997:Osmotic Pressure; D011205:Povidone; D011309:Preservation, Biological; D012769:Shock
D003451:Cryoprotective Agents; D011205:Povidone; D005990:Glycerol; D004121:Dimethyl Sulfoxide
Konservierung von hämopoetischen Stammzellen