Local aggregation of hormone-receptor complexes is required for activation by epidermal growth factor.
An analogue of epidermal growth factor (EGF) which is virtually devoid of biological activity retains receptor binding activity but cannot form cell surface clusters or patches. Bivalent anti-EGF antibodies restore both bioactivity and patch formation. The sensitivity of fibroblasts to native EGF can also be enhanced greatly by these antibodies, especially in hormone-resistant cell lines.
D000818:Animals; D002460:Cell Line; D003488:Cyanogen Bromide; D004815:Epidermal Growth Factor / Q000276:immunology / Q000378:metabolism*; D007152:Immunologic Capping; D007158:Immunologic Techniques; D008560:Membrane Fluidity; D051379:Mice; D008856:Microscopy, Fluorescence; D010446:Peptide Fragments / Q000378:metabolism; D010455:Peptides / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D013329:Structure-Activity Relationship
D010446:Peptide Fragments; D010455:Peptides; D011956:Receptors, Cell Surface; D004815:Epidermal Growth Factor; D003488:Cyanogen Bromide