title: Growth response of axenic Entamoeba histolytica to hydrogen, carbon dioxide, and oxygen.
abstract: Entamoeba histolytica required CO2 for growth in axenic culture while growth was inhibited by H2. The organism was tolerant to 5% O2 in the gas phase and it was able to detoxify products of O2 reduction in the medium. The ameba did not require a negative oxidation-reduction potential for axenic growth. However, little or no free O2 was present in media exposed to 5% O2 in the gas phase. Growth was improved by adding yeast extract to the medium.
mesh_terms: D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism; D003470:Culture Media; D003545:Cysteine / Q000378:metabolism; D004748:Entamoeba histolytica / Q000254:growth & development*; D005856:Germ-Free Life; D006859:Hydrogen / Q000378:metabolism; D010084:Oxidation-Reduction; D010101:Oxygen Consumption
chemical_list: D003470:Culture Media; D002245:Carbon Dioxide; D006859:Hydrogen; D003545:Cysteine