Chemical modification of bovine heart mitochondrial malate dehydrogenase.  Selective modification of cysteine and histidine.
Bovine mitochondrial malate dehydrogenase (EC 1.1.1.37) was inactivated by the specific modifications of a single histidine residue upon reaction with iodoacetamide.  NADH protected against this loss of activity and reaction with the histidine residue, suggesting that the histidine is at the NADH binding site.  N-Ethylmaleimide also modified the enzyme by reacting with 1 sulfhydryl residue.  The reaction rate with N-ethylmaleimide was increased by decreasing the pH from neutrality or by the addition of urea.  NADH protected against the modification of the sulfhydryl group under all the conditions tested, again suggesting active site specificity for this inactivation.  This enzyme has a subunit weight of 33,000 and is a dimer.  The native malate dehydrogenase will bind only 1 mol of NADH and it is thus assumed that there is only a single active site per dimer.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002250:Carbon Radioisotopes; D002417:Cattle; D003545:Cysteine / Q000378:metabolism*; D005033:Ethylmaleimide / Q000494:pharmacology; D006639:Histidine / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007460:Iodoacetamide / Q000494:pharmacology; D007461:Iodoacetates / Q000494:pharmacology; D008291:Malate Dehydrogenase / Q000378:metabolism*; D008293:Malates / Q000494:pharmacology; D008931:Mitochondria, Muscle / Q000201:enzymology*; D008970:Molecular Weight; D009206:Myocardium / Q000201:enzymology; D009243:NAD / Q000494:pharmacology; D010071:Oxaloacetates / Q000494:pharmacology; D014508:Urea / Q000494:pharmacology
D000596:Amino Acids; D002250:Carbon Radioisotopes; D007461:Iodoacetates; D008293:Malates; D010071:Oxaloacetates; D009243:NAD; D006639:Histidine; D014508:Urea; D008291:Malate Dehydrogenase; D003545:Cysteine; D005033:Ethylmaleimide; D007460:Iodoacetamide