Immunocytochemical evidence for anti-LHRH and anti-ACTH activity in the "F" antiserum.
An antiserum which has previously been thought to be specific for LHRH-like immunoreactive material was shown to contain two populations of antibodies, one demonstrating anti-LHRH activity and the other anti-ACTH(1-24) activity. In rat and mouse, ACTH(1-24)-like immunoreactive substance is present in perikarya within the basal hypothalamus and in fibers in arcuate, periventricular and dorsomedial nuclei. LHRH-like immunoreactivity is present in fibers within the median eminence and arcuate nucleus, in a few fibers running along the ventral border of the hypothalamus, and in a small number of cell bodies within the medial basal hypothalamus.
D000324:Adrenocorticotropic Hormone / Q000276:immunology*; D000818:Animals; D000918:Antibody Specificity; D007987:Gonadotropin-Releasing Hormone / Q000276:immunology*; D006651:Histocytochemistry; D007031:Hypothalamus / Q000276:immunology; D007106:Immune Sera / Q000276:immunology*; D007158:Immunologic Techniques; D008297:Male; D051379:Mice; D051381:Rats
D007106:Immune Sera; D007987:Gonadotropin-Releasing Hormone; D000324:Adrenocorticotropic Hormone