Anomalous vitamin E effects in mitochondrial oxidative metabolism.
Three different vitamin E effects, suggestive of specific antioxidant effects, were discovered in the protective action of vitamin E against respiratory decline (a decrease in mitochondrial respiration attributed to a "leakage" of electron transport radicals). No correlation was found between respiraotry decline and random lipid peroxidation. The mechanisms behind two of the three atypical vitamin E effects were defined. Both involve an artifact in the TBA assay for lipid peroxidation. This artifact occurs when TBA assays are carried out in the presence of sucrose and acetaldehyde; the latter is produced from ethanol, the solvent used to add vitamin E to preparations. The artifact in the TBA assay for peroxidations appears also to be responsible for differing interpretations of the hepatotoxic effect of ethanol.
D000079:Acetaldehyde / Q000378:metabolism; D000818:Animals; D002458:Cell Fractionation; D003600:Cytosol; D000431:Ethanol / Q000494:pharmacology; D050356:Lipid Metabolism; D008297:Male; D008862:Microsomes, Liver; D008930:Mitochondria, Liver / Q000378:metabolism*; D008933:Mitochondrial Swelling / Q000187:drug effects; D009243:NAD / Q000494:pharmacology; D010101:Oxygen Consumption / Q000187:drug effects*; D051381:Rats; D013858:Thiobarbiturates / Q000378:metabolism; D014810:Vitamin E / Q000494:pharmacology*
D013858:Thiobarbiturates; D009243:NAD; D014810:Vitamin E; D000431:Ethanol; D000079:Acetaldehyde