[Transformation of human embryonic cells by the Rous sarcoma virus and the polyomavirus depending on the mitotic cycle phase].
The whole cycle of skin-muscle embryonic human tissue culture is 18 hours, with phases S, G1, G2 and M being 7, 6, 4 and 1 hour, respectively. The mitotic index of this culture is 28%. The maximum sensitivity of these synchronized cell cultures to transforming activity of the Rous and Sindai viruses was observed in phase S. The infection of synchronized primary embryonic human fibroblasts in phase S with the polyoma virus together with the Sindai virus has resulted in single cases of transformation. Similar results were obtained with non-synchronized human cultures.
D000327:Adsorption; D001345:Autoradiography; D001358:Avian Sarcoma Viruses / Q000472:pathogenicity*; D002453:Cell Cycle; D002472:Cell Transformation, Viral; D006801:Humans; D007399:Interphase; D008938:Mitosis; D010222:Parainfluenza Virus 1, Human / Q000472:pathogenicity; D011120:Polyomavirus / Q000472:pathogenicity*; D013997:Time Factors; D014776:Virus Cultivation
Transformatsiia émbrional'nykh kletok cheloveka virusom sarkomy Rausa i virusom poliomy v zavisimosti ot fazy mitoticheskogo tsikla.