Synthesis of renin substrate by rat liver.
The present study attempts to determine if the isolated rat liver is capable of synthesizing renin substrate from 14C-labelled amino acids added in the perfusate. The renin substrate is characterized via reaction with renin, forming a substance that is subsequently identified as proangiotensin. Extensive evaluation of the reaction product is carried out by using molecular-sieve chromatography, countercurrent distribution, reactivity with converting enzyme, radioimmunological technique and bioassay. The results demonstrate that isolated rat liver perfused with artificial salt solution is capable of synthesizing a protein that reacts with renin to form a radioactive substance indistinguishable from proangiotensin.
D000808:Angiotensinogen / Q000096:biosynthesis* / Q000302:isolation & purification; D000809:Angiotensins / Q000096:biosynthesis*; D000818:Animals; D003377:Countercurrent Distribution; D005260:Female; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism*; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism; D011498:Protein Precursors / Q000302:isolation & purification / Q000378:metabolism; D051381:Rats; D012083:Renin / Q000378:metabolism
D000809:Angiotensins; D011498:Protein Precursors; D000808:Angiotensinogen; D007703:Peptidyl-Dipeptidase A; D012083:Renin