Episomal viral DNA in herpesvirus saimiri-transformed lymphoid cell lines.
Structural analysis of episomal viral genomes from two herpesvirus saimiri (HVS)-transformed tumour cell lines (No. 1670 and 70N2) showed that both types of episomes have a higher molecular weight than linear virion DNA. The arrangement of unique (L) and repetitive (H) DNA in No. 1670 episomes was studied by partial denaturation mapping. Part of the L-sequences present in linear virion DNA was found to be missing, part was found to be duplicated in the episomes. The episomal L-DNA regions were correlated with the known physical gene maps of linear HVS DNA.
D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002874:Chromosome Mapping; D004278:DNA, Superhelical / Q000378:metabolism; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism*; D005814:Genes, Viral; D006564:Herpesviridae / Q000378:metabolism*; D006567:Herpesvirus 2, Saimiriine / Q000235:genetics / Q000378:metabolism*; D008970:Molecular Weight; D010957:Plasmids; D013601:T-Lymphocytes
D004278:DNA, Superhelical; D004279:DNA, Viral