The influence of fluoro-phenyl-alanine on the synthesis of simian virus 40 DNA and T antigens.
Treatment of Simian Virus 40 (SV40) infected monkey cells with fluorophenylalanine (FPA) resulted in increased uptake of thymidine by the cells, and progressive inhibition of both viral and cellular DNA synthesis. Viral DNA synthesis was more sensitive to inhibition by FPA than cell DNA synthesis. Synthesis of SV40 T antigens was however unaffected by FPA, as judged from immunofluorescence assays. The M.W. of the major polypetides immunoprecipitated from cell extracts by antibodies from tumor bearing hamster sera was similarly unaffected. It is suggested that T antigen synthesized in the presence of FPA is non functional.
D000818:Animals; D000956:Antigens, Viral; D002460:Cell Line; D002472:Cell Transformation, Viral; D004261:DNA Replication / Q000187:drug effects; D004279:DNA, Viral / Q000096:biosynthesis*; D000882:Haplorhini; D007700:Kinetics; D010649:Phenylalanine / Q000031:analogs & derivatives*; D013539:Simian virus 40 / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D010135:p-Fluorophenylalanine / Q000494:pharmacology*
D000956:Antigens, Viral; D004279:DNA, Viral; D010649:Phenylalanine; D010135:p-Fluorophenylalanine