title: Tetrahedral intermediate in a specific alpha-chymotrypsin inhibitor complex detected by laser Raman spectroscopy.
abstract: Laser Raman spectroscopy indicates that inhibition of alpha-chymotrypsin by 2-phenylethaneboronic acid occurs in two steps: (i) the formation of a loosely bound complex, in which the boron remains in a trigonal configuration; and (ii) reaction of the boron with a functional group in the catalytic site of the enzyme, resulting in a reversible, stable tetrahedral adduct.
mesh_terms: D001665:Binding Sites; D001897:Boronic Acids / Q000494:pharmacology*; D002918:Chymotrypsin / Q000037:antagonists & inhibitors*; D006863:Hydrogen-Ion Concentration; D007834:Lasers; D013057:Spectrum Analysis
chemical_list: D001897:Boronic Acids; D002918:Chymotrypsin