Separation and molecular weight estimation of phosphatases in psoriatic scales by disc-electrophoresis.
Disc-electrophoretic investigations of psoriatic scale homogenates (15000 x g supernatant) revealed several different phosphatase activities. At pH 5 either five different phosphatase active bands (substrates: p-nitrophenylphosphate, naphthylphosphate) or three different bands (substrate: glycerophosphate) could be obtained. At pH 7 only one band (substrate: p-nitrophenylphosphate) showed phosphatase activity. At pH 9.9 either two bands (substrate: p-nitrophenylphosphate) or three bands (substrate: glycerophosphate) could be demonstrated. the acid p-nitrophenylphosphatase activity of the psoriatic specific bands "9" and "10" could be distinguished by their different fluoride and tartrate susceptibility. Also the alkaline glycerophosphatase activities could be differentiated by distinct Ca and Mg susceptibility.
D000135:Acid Phosphatase / Q000302:isolation & purification; D000469:Alkaline Phosphatase / Q000302:isolation & purification; D002118:Calcium; D003545:Cysteine; D004589:Electrophoresis, Disc; D005459:Fluorides; D005994:Glycerophosphates; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008274:Magnesium; D008970:Molecular Weight; D009284:Naphthols; D009596:Nitrophenols; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis / Q000037:antagonists & inhibitors / Q000302:isolation & purification*; D011565:Psoriasis / Q000201:enzymology*; D013644:Tartrates
D005994:Glycerophosphates; D009284:Naphthols; D009596:Nitrophenols; D013644:Tartrates; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D010744:Phosphoric Monoester Hydrolases; D008274:Magnesium; D003545:Cysteine; D005459:Fluorides; D002118:Calcium