Evaluation of a ribosomal vaccine against pertussis.
A crude ribosomal vaccine derived from Bordetella pertussis administered to ICR and N:NIH (SW) strains of mice protected them effectively against a standardized intracranial challenge. The dose of vaccine that protected half the mice was less for N:NIH (SW) than for ICR mice and compared favorably with a killed reference vaccine. Ribosomes prepared from bacteria ground with washed sea sand were more immunogenic than those obtained by rupture with alumina or with a Braun homogenizer. The protective effect of the crude ribosomes was not an innate part of the organelle but was due to a substance or substances that could be removed from them by a 1 M NH4Cl wash. The material in the wash was highly immunogenic and retained both the histamine-sensitizing and leukocytosis-promoting properties. It lost much of the dermonecrotic activity and was poorly pyrogenic in rabbits. The most potent pyrogen was present in the washed ribosomes, which apparently, retained the endotoxic components of the cell wall. The best vaccines permitted acceptable weight gain in the immunized mice.
D000818:Animals; D001835:Body Weight; D001886:Bordetella pertussis / Q000276:immunology / Q000648:ultrastructure; D002458:Cell Fractionation / Q000379:methods; D005260:Female; D006632:Histamine / Q000276:immunology; D007962:Leukocytes / Q000276:immunology; D051379:Mice; D010567:Pertussis Vaccine / Q000276:immunology* / Q000633:toxicity; D011749:Pyrogens; D012270:Ribosomes / Q000276:immunology*
D010567:Pertussis Vaccine; D011749:Pyrogens; D006632:Histamine