Effect of volume expansion on renal citrate and ammonia metabolism in KCl-deficient rats.
When rats with desoxycorticosterone acetate (DOCA)-induced potassium chloride deficiency are given sodium chloride there is simultaneously a partial correction of metabolic alkalosis and a marked reduction in urinary citrate excretion and renal citrate content. To examine DOCA's role in this phenomenon and to determine how sodium chloride alters renal metabolism, rats were made KC1 deficient using furosemide and a KC1-deficient diet. Renal citrate and ammonia metabolism were then studied after chronic oral sodium chloride administration or acute volume expansion with isotonic mannitol. Although both maneuvers partially corrected metabolic alkalosis, sodium chloride raised serum chloride concentration while mannitol significantly decreased it. Urinary citrate excretion decreased to 10% of control in rats given NaCl and to 50% of control in rats infused with mannitol. The filtered load of citrate was constant or increased indicating increased tubular citrate reabsorption. Renal cortical citrate content also decreased approximately 50%. Renal cortical slices from KCl-deficient rats incubated in low or normal chloride media produced equal amounts of 14CO2 from (1, 5-14C) citrate. In addition, urinary ammonia excretion increased by over 300% in both groups. This occurred in the mannitol group despite increased urinary pH and flow rate indicating a rise in renal ammonia production. It seems that neither DOCA nor an increase in serum chloride concentration explains the experimental results. Rather, it appears that volume expansion is responsible for increased renal tubular citrate reabsorption and renal ammonia production. As these renal metabolic responses ordinarily occur in response to acidosis, the data are consistent with the hypothesis that volume expansion reduces renal cell pH in 3KCl-deficient rats.
D000641:Ammonia / Q000378:metabolism*; D000818:Animals; D002951:Citrates / Q000378:metabolism*; D005665:Furosemide / Q000494:pharmacology; D005919:Glomerular Filtration Rate; D006863:Hydrogen-Ion Concentration; D007668:Kidney / Q000032:analysis / Q000378:metabolism*; D008099:Liver / Q000032:analysis; D008297:Male; D008353:Mannitol / Q000494:pharmacology; D009132:Muscles / Q000032:analysis; D009206:Myocardium / Q000032:analysis; D011188:Potassium / Q000032:analysis; D011189:Potassium Chloride; D011191:Potassium Deficiency / Q000378:metabolism*; D051381:Rats; D012965:Sodium Chloride / Q000494:pharmacology*
D002951:Citrates; D008353:Mannitol; D012965:Sodium Chloride; D011189:Potassium Chloride; D000641:Ammonia; D005665:Furosemide; D011188:Potassium