Biotin carboxyl carrier protein in barley chloroplast membranes.
Biotin localized in barley chloroplast lamellae is covalently bound to a single protein with an approximate molecular weight of 21 000. It contains one mole of biotin per mole of protein and functions as a carboxyl carrier in the acetyl-CoA carboxylase reaction. The protein was obtained by solubilization of the lamellae in phenol/acetic acid/8 M urea. Feeding barley seedlings with [14C]-biotin revealed that the vitamin is not degraded into respiratory substrates by the plant, but is specifically incorporated into biotin carboxyl carrier protein.
D000103:Acetyl-CoA Carboxylase; D001710:Biotin / Q000378:metabolism; D002352:Carrier Proteins / Q000032:analysis* / Q000302:isolation & purification / Q000378:metabolism; D002736:Chloroplasts / Q000032:analysis* / Q000201:enzymology / Q000378:metabolism / Q000648:ultrastructure; D002854:Chromatography, Paper; D002855:Chromatography, Thin Layer; D004589:Electrophoresis, Disc; D008566:Membranes / Q000032:analysis; D008970:Molecular Weight; D010944:Plants / Q000648:ultrastructure; D012995:Solubility; D013347:Subcellular Fractions / Q000032:analysis / Q000201:enzymology
D002352:Carrier Proteins; D001710:Biotin; D000103:Acetyl-CoA Carboxylase