The structural gene for NADP L-glutamate dehydrogenase in Aspergillus nidulans.
A total of 41 mutants lacking NADP L-glutamate dehydrogenase (NADP-GDH) activity have been studied. All the mutations were located at the gdhA locus within 0-1% recombination of gdhAI. Two mutants, gdhAI and gdhA2, out of five examined, produced cross-reacting material which neutralized NADP-GDH anti-serum. The mutant gdhA9 has altered Km values for all five substrates: ammonium, alpha-ketoglutarate, l-glutamate, NADPH and NADP. The mutant gdhA20 had temperature-sensitive growth, abnormal ammonium-regulation characteristics and thermolabile NADP-GDH activity. These results show that gdhA is the structural gene for NADP-GDH.
D000445:Aldehyde Oxidoreductases / Q000378:metabolism; D000641:Ammonia / Q000378:metabolism; D001233:Aspergillus nidulans / Q000201:enzymology* / Q000378:metabolism; D002474:Cell-Free System; D002874:Chromosome Mapping; D003433:Crosses, Genetic; D004592:Electrophoresis, Starch Gel; D005796:Genes; D005816:Genetic Complementation Test; D005969:Glutamate Dehydrogenase / Q000096:biosynthesis* / Q000378:metabolism; D005971:Glutamates / Q000378:metabolism; D007656:Ketoglutaric Acids / Q000378:metabolism; D009154:Mutation; D009249:NADP / Q000378:metabolism; D009565:Nitrate Reductases / Q000378:metabolism; D009566:Nitrates / Q000378:metabolism; D009604:Nitrosoguanidines; D011995:Recombination, Genetic; D013237:Stereoisomerism; D013890:Thiourea / Q000378:metabolism
D005971:Glutamates; D007656:Ketoglutaric Acids; D009566:Nitrates; D009604:Nitrosoguanidines; D009249:NADP; D000641:Ammonia; D000445:Aldehyde Oxidoreductases; D005969:Glutamate Dehydrogenase; D009565:Nitrate Reductases; D013890:Thiourea