[Effect of various methods of preparation of chromatin on the distribution of Mn2+-dependent poly-A-polymerase activity in subnuclear fractions of rat liver].
The distribution of Mn2+-dependent poly(A) polymerase activity between chromatin and nucleoplasmic fractions in rat liver nuclei strongly depends on the method followed for the chromatin preparation. Currently used methods containing high salt cause a loss in bound activity not accompanied by a correspondent increase in the "free" one.
D000818:Animals; D002467:Cell Nucleus / Q000201:enzymology*; D002843:Chromatin / Q000032:analysis / Q000201:enzymology*; D008099:Liver / Q000201:enzymology*; D008345:Manganese / Q000494:pharmacology; D009713:Nucleotidyltransferases / Q000032:analysis*; D011062:Polynucleotide Adenylyltransferase / Q000032:analysis*; D051381:Rats; D013347:Subcellular Fractions / Q000201:enzymology
D002843:Chromatin; D008345:Manganese; D009713:Nucleotidyltransferases; D011062:Polynucleotide Adenylyltransferase
Effetto di vari metodi di preparazione della cromatina sulla distribuzione dell'attività poli (A) polimerasi Mn2+-dipendente in frazioni subnucleari di fegato di ratto.