Human corticotropin (ACTH) radioimmunoassay with synthetic 1--24 ACTH.
A corticotropin antiserum was obtained from rabbits immunized with synthetic 1--24 corticotropin conjugated with bovine serum albumin. The antiserum did not cross react with synthetic alpha-melanotropin or with synthetic beta-endorphin and had a cross reactivity of 0.23% with human beta-lipotropin. We developed a radioimmunoassay with the antiserum obtained, in which we used polyethylene glycol in conjunction with a second precipitating antibody for fast (15-min) separation of antibody-bound and free corticotropin. The assay had a sensitivity of 16 ng/L and was validated on patients with various pituitary and adrenal diseases. From 103 normal subjects, the median value for corticotropin in specimens collected during the morning was 34 ng/L of plasma; the upper 95% confidence limit of the normal range was 98 ng/L.
D000307:Adrenal Gland Diseases / Q000097:blood; D000324:Adrenocorticotropic Hormone / Q000031:analogs & derivatives* / Q000097:blood*; D000818:Animals; D003366:Cosyntropin / Q000276:immunology*; D003429:Cross Reactions; D005260:Female; D006801:Humans; D007003:Hypoglycemia / Q000175:diagnosis; D007106:Immune Sera; D007328:Insulin; D007457:Iodine Radioisotopes; D008297:Male; D009369:Neoplasms / Q000032:analysis; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay; D012016:Reference Values
D007106:Immune Sera; D007328:Insulin; D007457:Iodine Radioisotopes; D003366:Cosyntropin; D000324:Adrenocorticotropic Hormone