[Purification of human gastric glycoprotein and a study of its carbohydrate components (author's transl)].
Mucus was extracted from human gastric mucosa by homogenization in distilled water. The crude extract was purified from plasma, salivary and tissue contaminants by different steps involving chromatography on Sepharose 6B, Sepharose 2B and immunosorbents. The native glycoprotein so prepared was found to be immunologically pure; it migrated as a single band in acrylamide agarose gel electrophoresis at pH 8.6 and 3.5. The molecule exhibits blood group acitivity, contains 0.71 per cent sialic acid but no sulphate. The carbohydrate moiety contains glucosamine, galactosamine, fucose, galatose and mannose in the molar proportions 3: 1.95: 2.92:4.53:0.05.
D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005643:Fucose / Q000032:analysis; D005688:Galactosamine / Q000032:analysis; D005690:Galactose / Q000032:analysis; D005753:Gastric Mucosa / Q000032:analysis*; D005944:Glucosamine / Q000032:analysis; D006023:Glycoproteins / Q000302:isolation & purification; D006801:Humans; D006863:Hydrogen-Ion Concentration; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D008358:Mannose / Q000032:analysis; D009093:Mucus / Q000032:analysis*; D012794:Sialic Acids / Q000032:analysis
D006023:Glycoproteins; D012794:Sialic Acids; D005643:Fucose; D005688:Galactosamine; D005944:Glucosamine; D008358:Mannose; D005690:Galactose
Purification d'une glycoprotéine gastrique humaine et étude de ses composants glucidiques.