Comparative studies of the physical state of the lipid phase of normal and hypercholesterolemic very low density lipoprotein.
Very low density lipoproteins (VLDL) were prepared from the serum of rabbits at various stages of hypercholesterolemia (95--1665 mg cholesterol/100 ml of serum). The most notable chemical change in hypercholesterolemic (hc) VLDL was the greatly increased content of cholesteryl esters and the greatly decreased content of triglycerides, compared to normal (n) VLDL. Structurally, the lipid region of n VLDL possessed a much lower microviscosity than did hc VLDL, when analyzed by fluorescence polarization and pyrene eximer methods. The microviscosity of the redispersed n VLDL lipid extract was considerably greater than the observed in n VLDL; but less than that of hc VLDL. Incorporation of pyrene into the lipid region of n VLDL and hc VLDL allowed assessment of various properties of the surface and hydrocarbon regions of these lipoproteins. Only slight differences were found in the pyrene monomer 3 : 1 fluorescence emission peak ratios, and in the rate constant for quenching of pyrene by O2. However, the quenching rate constant of pyrene by I- and iodoheptane were different for each lipoprotein.
D000818:Animals; D002784:Cholesterol / Q000097:blood; D002788:Cholesterol Esters / Q000097:blood; D006937:Hypercholesterolemia / Q000097:blood*; D008079:Lipoproteins, VLDL / Q000097:blood*; D010743:Phospholipids / Q000097:blood; D011817:Rabbits; D012016:Reference Values; D014280:Triglycerides / Q000097:blood
D002788:Cholesterol Esters; D008079:Lipoproteins, VLDL; D010743:Phospholipids; D014280:Triglycerides; D002784:Cholesterol