title: Studies on 15-hydroxyprostaglandin dehydrogenase with various prostaglandin analogues.
abstract: The NAD+-linked 15-hydroxyprostaglandin dehydrogenase (PGDH) of swine lung was purified to a high specific activity by affinity chromatographies on prostaglandin (PG)-and NAD+-Sepharose. The affinities of the enzyme for various synthetic analogues of PGA, E, F, and I and their inhibitory effects on the enzymatic reaction were examined. The modification of the alkyl side chain of PG, particularly at C-15 or C-16, reduced the affinity of the enzyme for these PG analogues. Furthermore, 14-methyl-13,14-dihydro-PGE1 and 16-cyclopentyl-omega-trinor-15-epi-PGE2 were potent inhibitors of PGDH.
mesh_terms: D000818:Animals; D002412:Cations; D006911:Hydroxyprostaglandin Dehydrogenases / Q000302:isolation & purification / Q000378:metabolism*; D008168:Lung / Q000201:enzymology; D009243:NAD; D011453:Prostaglandins; D013379:Substrate Specificity; D013552:Swine
chemical_list: D002412:Cations; D011453:Prostaglandins; D009243:NAD; D006911:Hydroxyprostaglandin Dehydrogenases