A comparison of canine normal hepatic alkaline phosphatase and variant alkaline phosphatase of serum and liver.
The isoenzyme of alkaline phosphatase from normal liver, the corticosteroid induced isoenzyme of alkaline phosphatase from serum and liver and a hepatocellular variant isoenzyme of alkaline phosphatase induced by lymphosarcoma have been partially purified and their the present modification incorporates Polybrene into buffer to eliminate this heparin interference. The proposed method shown excellent agreement with a reference procedure based on clottable protein, and excellent day-to-day precision (C.V.3.5%). The present method is easily adaptable to semi-automated measurements.
D000303:Adrenal Cortex Diseases / Q000201:enzymology; D000305:Adrenal Cortex Hormones / Q000494:pharmacology; D000469:Alkaline Phosphatase / Q000097:blood / Q000378:metabolism*; D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000201:enzymology; D004285:Dogs; D004355:Drug Stability; D004790:Enzyme Induction / Q000187:drug effects; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000097:blood / Q000378:metabolism*; D007700:Kinetics; D007930:Leucine / Q000494:pharmacology; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008113:Liver Neoplasms; D008228:Lymphoma, Non-Hodgkin / Q000201:enzymology; D009362:Neoplasm Metastasis; D010649:Phenylalanine / Q000494:pharmacology; D013997:Time Factors
D000305:Adrenal Cortex Hormones; D007527:Isoenzymes; D010649:Phenylalanine; D000469:Alkaline Phosphatase; D007930:Leucine