title: Morphogenesis of nuclear inclusions and virus capsids in HEL cells infected with temperature-sensitive mutants of human cytomegalovirus.
abstract: The morphogenesis of nuclear inclusions and virus capsids in human embryonic lung cells infected with ts mutants of human cytomegalovirus at permissive (34 degrees C) and non-permissive (39 degrees C) temperatures was studied by indirect immunofluorescence (IF) and electron microscopic analyses and compared with the morphogenesis of these structures in wild-type virus infection with or without phosphonoacetate. Mutants tested belonged to five different complementation groups: two groups were DNA- (those unable to synthesize virus DNA at 39 degrees C) and the others were dna+. Based on the previous finding that the electron-dense, reticular nuclear inclusions (EM-NI) observed by the thin-section analysis correspond with nuclear inclusions (IF-NI) detected by the indirect IF staining (i.e. they occupy the same space in the nucleus), the following conclusions were obtained in ts mutant infection at 39 degrees C: (i) the formation of EM-NI, IF-NI and virus capsids requires replication of virus DNA. (II) The formation of EM-NI is not necessarily accompanied by the formation of IF-NI; EM-NI itself is not IF-positive unless it acquires virus-specific late antigens. (iii) The assembly of virus capsids occurs only in those cells in which EM-NI is formed; however, it can occur without the formation of IF-NI. (iv) Virus capsids assembled are not the major antigens responsible for the fluorescence of nuclear inclusions.
mesh_terms: D002213:Capsid / Q000096:biosynthesis*; D002460:Cell Line; D003587:Cytomegalovirus / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D004279:DNA, Viral / Q000096:biosynthesis*; D005814:Genes, Viral; D006801:Humans; D007181:Inclusion Bodies, Viral / Q000378:metabolism*; D008168:Lung; D009024:Morphogenesis; D009154:Mutation; D013696:Temperature; D014764:Viral Proteins / Q000096:biosynthesis*
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins