Multiplication of normal and virus-infected diploid cells cultivated on microcarriers.
In prelminary studies, growth of diploid cells (fetal calf buccal cells and chick embryo fibroblasts on dextran microcarriers was studied and compared to growth in monolayer. Growth coefficients obtained were always greater (by 2 fold) when microcarriers were used. Moreover, this technique had the advantage of reducing the number of manipulations and hence the risk of contamination. In two series of experiments, buccal epithelial cells were infected with vescicular stomatitis virus. Good virus multiplication was obtained.
D000818:Animals; D002417:Cattle; D002455:Cell Division; D002460:Cell Line; D002478:Cells, Cultured; D002642:Chick Embryo; D046508:Culture Techniques / Q000379:methods*; D004171:Diploidy; D005347:Fibroblasts; D009061:Mouth Mucosa / Q000196:embryology; D014721:Vesicular stomatitis Indiana virus / Q000254:growth & development*; D014779:Virus Replication