title: A general priming system employing only dnaB protein and primase for DNA replication.
abstract: Priming of phage phi X174 DNA synthesis is effected simply by dnaB protein and primase when the DNA is not coated by single-strand binding protein (SSB). The five prepriming proteins (n,n',n'',i, and dnaC protein) required for priming a SSB-coated phi X174 DNA circle are dispensable. The dnaB protein-primase priming system is also active on uncoated phage G4 and M13 DNAs and on poly(dT). Multiple RNA primers, 10--60 nucleotides long, are transcribed with patterns distinctive for each DNA template. Formation of a stable dnaB protein.DNA complex in the presence of primase and ATP supports the hypothesis that dnaB protein provides a mobile replication promoter signal for primase.
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D010584:Bacteriophage phi X 174 / Q000235:genetics; D003090:Coliphages / Q000235:genetics*; D004265:DNA Helicases / Q000378:metabolism*; D004258:DNA Polymerase III / Q000378:metabolism; D004261:DNA Replication; D004277:DNA, Single-Stranded / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D011071:Poly T / Q000378:metabolism; D013698:Templates, Genetic; D014779:Virus Replication
chemical_list: D001426:Bacterial Proteins; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D011071:Poly T; D004258:DNA Polymerase III; D004265:DNA Helicases