The time course of the phosphorylation of proteins in the synaptic plasma membrane and the effect of certain cations.
The phosphorylation of proteins in the synaptic plasma membrane is a rather slow reaction taking several minutes to saturate all the phosphate acceptor sites. (The time for half the protein bound phosphate groups to turnover is about 1 min). A divalent cation is needed as a cofactor for the reaction. At high (0.5 mM) ATP concentrations Mg2+ is more effective than Mn2+ but at low (10 microM) ATP concentrations the reverse is the case. Zn2+ and Ca2+ support very little phosphorylation.
D000255:Adenosine Triphosphate / Q000008:administration & dosage / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002413:Cations, Divalent / Q000494:pharmacology*; D000242:Cyclic AMP / Q000494:pharmacology; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D008565:Membrane Proteins / Q000378:metabolism; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism*; D051381:Rats; D013570:Synaptic Membranes / Q000378:metabolism*; D013997:Time Factors; D015032:Zinc / Q000494:pharmacology
D002413:Cations, Divalent; D008565:Membrane Proteins; D008345:Manganese; D000255:Adenosine Triphosphate; D000242:Cyclic AMP; D011494:Protein Kinases; D008274:Magnesium; D015032:Zinc; D002118:Calcium