Solubilized and insolubilized bone morphogenetic protein.
A bone morphogenetic protein (BMP) obtained in solution by digestion of demineralized rabbit cortical bone matrix with bacterial collagenase retains its biologically active conformation in a neutral salt/ethylene glycol mixture. BMP may be insolubilized by coprecipitation with calcium phosphate and resolubilized by chemical extraction with a neutral salt in the same solvent mixture. Upon concanavalin A-Sepharose chromatography, BMP is bound by hydrophobic interaction and carbohydrate recognition and is recovered by elution with either alpha-methyl mannoside or ethylene glycol solvent mixture. Implants of both eluates and the extracts of the coprecipitate in double-walled diffusion chambers induce transmembrane bone morphogenesis. BMP is not species specific; rabbit BMP induces new bone formation in the rat. The present observations indicate that BMP is a glycoprotein.
D000818:Animals; D001681:Biological Assay; D001842:Bone and Bones / Q000502:physiology*; D003012:Microbial Collagenase; D011487:Protein Conformation; D011506:Proteins / Q000302:isolation & purification / Q000502:physiology*; D011817:Rabbits; D012995:Solubility
D011506:Proteins; D003012:Microbial Collagenase