Rapid fluorimetric determination of 5-fluorocytosine in serum.
A rapid fluorimetric procedure for the determination of the fungicide 5-fluorocytosine in serum is described. The method is high in precision (S.D. 1.6 mug/ml at 50 mug/ml) and specificity and can be performed in 60-90 minutes. The results obtained for samples from six patients showed excellent agreement with those obtained by traditional microbiological methods.
D001681:Biological Assay; D002176:Candida albicans / Q000187:drug effects; D003596:Cytosine / Q000031:analogs & derivatives*; D005069:Evaluation Studies as Topic; D005437:Flucytosine / Q000097:blood* / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008832:Microchemistry; D012441:Saccharomyces cerevisiae / Q000187:drug effects; D013050:Spectrometry, Fluorescence / Q000379:methods
D003596:Cytosine; D005437:Flucytosine