title: DNA-mediated transfer of herpes simplex virus TK gene to human TK- cells: properties of the transformed lines.
abstract: Human TK- cells carrying the HSV-2 TK gene express a TK activity of viral origin and maintain the TK+ phenotype when grown in HAT medium. Under non-selective or counterselective conditions, however, reversion to a TK- phenotype occurs with a significant frequency characteristic of each transformed line. The TK- phenotype appears to be stable since no instances of TK- to TK+ reversion have been observed.
mesh_terms: D001973:Bromodeoxyuridine / Q000008:administration & dosage; D002460:Cell Line; D002472:Cell Transformation, Viral; D003470:Culture Media; D004279:DNA, Viral / Q000235:genetics*; D005814:Genes, Viral; D006801:Humans; D010641:Phenotype; D018139:Simplexvirus / Q000201:enzymology / Q000235:genetics*; D013937:Thymidine Kinase / Q000235:genetics*; D014162:Transfection
chemical_list: D003470:Culture Media; D004279:DNA, Viral; D013937:Thymidine Kinase; D001973:Bromodeoxyuridine