A radiochemical method for the determination of transketolase activity in erythrocyte hemolysates.
A radiochemical method for the determination of transketolase activity is reported. It is based on incubation of erythrocyte hemolysates with radioactive ribose 5-phosphate followed by isolation of sedoheptulose 7-phosphate on anion-exchange columns. The experimental conditions are discussed and the presence of phosphatase activity in the incubation mixture is demonstrated. The proposed method is compared with a current colorimetric method. Reference values are given for the transketolase activity and the thiamine pyrophosphate effect.
D000328:Adult; D003124:Colorimetry; D004912:Erythrocytes / Q000201:enzymology*; D005260:Female; D006461:Hemolysis; D006801:Humans; D008297:Male; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis; D011854:Radiochemistry / Q000379:methods; D012267:Ribosemonophosphates; D013835:Thiamine Pyrophosphate / Q000494:pharmacology; D014174:Transketolase / Q000097:blood*
D012267:Ribosemonophosphates; D014174:Transketolase; D010744:Phosphoric Monoester Hydrolases; D013835:Thiamine Pyrophosphate