High-resolution proton nuclear magnetic resonance studies of sickle cell hemoglobin.
High-resoluiton proton nuclear magnetic resonance spectroscopy at 250 MHz has been used to investigate sickle cell hemoglobin. The hyperfine shifted, the ring-current shifted, and the exchangeable proton resonances suggest that the heme environment and the subunit interfaces of the sickle cell hemoglobin molecule are normal. These results suggest that the low oxygen affinity in sickle cell blood is not due to conformational alterations in the heme environment or the subunit interfaces. The C-2 proton resonances of certain histidyl residues can serve as structural probes for the surface conformation of the hemoglobin molecule. Several sharp resonances in sickle cell hemoglobin are shifted upfield from their positions in normal adult hemoglobin. These upfield shifts, which are observed in both oxy and deoxy forms of the molecule under various experimental conditions, suggest that some of the surface residues of sickle cell hemoglobin are altered and they may be in a more hydrophobic environment as compared with that of normal human adult hemoglobin. These differences in surface conformation are pH and ionic strength specific. In particular, upon the addition of organic phosphates to normal and sickle cell hemoglobin samples, the differences in their aromatic proton resonances diminish. These changes in the surface conformation may, in part, be responsible for the abnormal properties of sickle cell hemoglobin.
D000328:Adult; D001665:Binding Sites; D002263:Carboxyhemoglobin; D004163:Diphosphoglyceric Acids / Q000097:blood; D006451:Hemoglobin, Sickle; D006454:Hemoglobins; D006455:Hemoglobins, Abnormal; D006579:Heterozygote; D006639:Histidine / Q000032:analysis; D006720:Homozygote; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D009994:Osmolar Concentration; D010100:Oxygen / Q000097:blood; D010833:Phytic Acid / Q000097:blood; D011485:Protein Binding; D011487:Protein Conformation
D004163:Diphosphoglyceric Acids; D006451:Hemoglobin, Sickle; D006454:Hemoglobins; D006455:Hemoglobins, Abnormal; D006639:Histidine; D010833:Phytic Acid; D002263:Carboxyhemoglobin; D010100:Oxygen