A poly(U) polymerase in tobacco leaves.
A poly(U) polymerizing enzyme has been found in healthy and tobacco mosaic virus-infected tobacco leaves and has been partially purified by affinity chromatography on a gel prepared from agarose with chemically coupled RNA. The enzyme is stimulated by Mn-2+ and dependent on a polynucleotide, preferentially poly(A). The synthesis proceeds optimally at pH 7.6 and 25 degrees C. The enzyme is highly specific for UTP and is inhibited by other ribonucleoside triphosphates. The product was partly sensitive to pancreatic ribonuclease. The synthetic reaction is inhibited in the presence of pyrophosphate but insensitive to 10 mM orthophosphate and high levels of cordycepin, rifampicin and actinomycin D. A molecular weight of about 40,000 has been estimated by sucrose gradient analysis and partition cell ultracentrifugation.
D000241:Adenosine / Q000031:analogs & derivatives / Q000494:pharmacology; D002846:Chromatography, Affinity; D004789:Enzyme Activation / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008345:Manganese / Q000494:pharmacology; D008970:Molecular Weight; D010944:Plants / Q000201:enzymology*; D010947:Plants, Toxic; D011072:Poly U / Q000096:biosynthesis*; D012316:RNA Nucleotidyltransferases / Q000302:isolation & purification / Q000378:metabolism*; D012260:Ribonucleases; D013696:Temperature; D014026:Nicotiana / Q000201:enzymology; D014027:Tobacco Mosaic Virus / Q000201:enzymology
D011072:Poly U; D008345:Manganese; D012316:RNA Nucleotidyltransferases; D012260:Ribonucleases; D000241:Adenosine