[Gamma-glutamyltransferase activity in the human red blood cell membrane (author's transl)].
A gamma-glutamyltransferase activity is found in the human red blood cell membrane. Membrane isolation was carried out according to the method of Dodge et al. (Dodge, J. T., Mitchell, C. and Hanahan, J. (1963) Arch. Biochem. Biophys. 100, 119-130) (modified) and proteins were solubilized either with 1% sodium deoxycholate or 5 mM EDTA or 10 mM of its disodium salt, under various conditions of time and temperature. The gamma-glutamyltransferase activity of the membrane preparations was investigated using two substrates, gamma-L-glutamyl-p-nitroanilide and gamma-L-glutamyl-alpha-naphthylamide. The specific enzymatic activities of the various preparations, expressed in m units per mg of protein, were found to have similar values under similar technical conditions. The chelating agents seem to allow a more specific isolation than the detergent. The presence of a gamma-glutamyltransferase activity in the erythrocyte membrane is discussed in relation to the membrane association of this enzyme in other tissues.
D002458:Cell Fractionation; D002462:Cell Membrane / Q000201:enzymology* / Q000648:ultrastructure; D003840:Deoxycholic Acid; D004492:Edetic Acid; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D007700:Kinetics; D012995:Solubility; D005723:gamma-Glutamyltransferase / Q000097:blood*
D003840:Deoxycholic Acid; D004492:Edetic Acid; D005723:gamma-Glutamyltransferase
Activité gamma-glutamyltransferase dans la membrane du globule rouge humain