Isolation of the globular region of the subcomponent q of the C1 component of complement.
Digestion after heat treatment of the subcomponent q of the C1 component of complement by collagenase leads to the isolation of the globular region of the protein. This product ('heads') is composed of three chains giving an overall molecular weight of about 57000. About half of the collagen-like region present in C1 q is lost after digestion. The 'heads' are shown to be soluble and hemolytically active products.
D000596:Amino Acids / Q000032:analysis; D003172:Complement C1; D006461:Hemolysis; D006595:Hexosamines / Q000032:analysis; D006801:Humans; D003012:Microbial Collagenase; D008970:Molecular Weight; D010447:Peptide Hydrolases; D011487:Protein Conformation; D013696:Temperature
D000596:Amino Acids; D003172:Complement C1; D006595:Hexosamines; D010447:Peptide Hydrolases; D003012:Microbial Collagenase