Studies on the mode of action of gonadotrophin-inhibiting material (anti-LH) isolated from human urine.
Gonadotrophin-inhibiting material (GIM) was able to inhibit the binding of 125I-hCG to rat Leydig cells, suggesting that its inhibiting properties are due to its ability to complete for the hCG binding sites on Leydig cells. Binding of fluorescein isothiocyanate-tagged GIM to Leydig cells was also seen. The effect of GIM on hCG-induced adenylate cyclase activation and testosterone production was also studied. It was found that there was a dose-dependent inhibition of both these effects.
D000262:Adenylyl Cyclases / Q000378:metabolism; D006063:Chorionic Gonadotropin / Q000378:metabolism; D004789:Enzyme Activation / Q000187:drug effects; D005452:Fluoresceins; D006023:Glycoproteins / Q000652:urine*; D006801:Humans; D007985:Leydig Cells / Q000187:drug effects / Q000378:metabolism; D007986:Luteinizing Hormone / Q000037:antagonists & inhibitors*; D008297:Male; D011956:Receptors, Cell Surface / Q000378:metabolism
D006063:Chorionic Gonadotropin; D005452:Fluoresceins; D006023:Glycoproteins; D011956:Receptors, Cell Surface; D007986:Luteinizing Hormone; D000262:Adenylyl Cyclases