Are polyphosphoinositides associated with glycophorin in human erythrocyte membranes?
Glycophorin prepared by a lithium di-iodosalicylate-extraction/phenol-partition method was rich in polyphosphoinositides (phosphatidyl-myo-inositol 4-phosphate and phosphatidyl-myo-inositol 4,5-bisphosphate), but glycophorin extracted by Triton X-100 showed no such enrichment. The enrichment observed in the former preparations appeared not to be caused by pre-existing association between glycophorin and polyphosphoinositides in the human erythrocyte membrane, but to be largely a consequence of the preparative procedures.
D006021:Glycophorins / Q000302:isolation & purification*; D006801:Humans; D010716:Phosphatidylinositols / Q000097:blood*; D010743:Phospholipids / Q000097:blood; D012795:Sialoglycoproteins / Q000302:isolation & purification*
D006021:Glycophorins; D010716:Phosphatidylinositols; D010743:Phospholipids; D012795:Sialoglycoproteins