Chimaerism of immunocompetent cells in allogeneic bone marrow-reconstituted lethally irradiated chickens.
Injection of parental bone marrow cells into 12-day-old lethally irradiated F1 hybrid chickens resulted in chimaerism of donor-type graft-versus-host (GVH)-reactive cells and suppression of antisheep red blood cell antibody response. These manifestations of a chronic graft-versus-host reaction were prevented by pretreatment of the donor marrow with specific anti-T cell globulin. In some chimaeras donor-type GVH-reactive cells developed gradually from T cells precursors of donor origin. Transplantation of spleen and marrow cells from sheep red blood cell-primed F1 hybrid donors into lethally irradiated parental recipients resulted in the loss of memory potential within 1-2 weeks of transfer, whereas donor-type IgG allotype synthesis was preserved. Injection of goat antichicken thymocyte serum to recipients 1 day before reconstitution enabled the antibody response of memory cells at 1-2 weeks, although it failed to prevent their rejection by 8-9 weeeks after transplantation. Split chimaerism of donor-type GVH-reactive cells was demonstrated in chickens which had previously rejected the B cells derived from the same graft.
D000818:Animals; D000917:Antibody Formation; D000921:Antibody-Producing Cells; D000961:Antilymphocyte Serum / Q000494:pharmacology; D001402:B-Lymphocytes / Q000276:immunology; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D002645:Chickens; D005260:Female; D006041:Goats / Q000276:immunology; D006087:Graft vs Host Reaction; D007156:Immunologic Memory; D007165:Immunosuppression Therapy; D007519:Isoantigens; D008297:Male; D009030:Mosaicism; D011828:Radiation Chimera; D013163:Splenomegaly / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D000961:Antilymphocyte Serum; D007519:Isoantigens