Effects of thyroid deficiency and sympathectomy on cardiac enzymes.
The effects of thyroid deficiency (Td) and of chemical sympathectomy (Sx) were studied on marker enzymes of energy metabolism in cardiac muscle of neonatal and of adult rats. Td prevented the normal development of neonatal body weight, relative heart mass, and cardiac levels of cytochrome c (-22%), citrate synthase (-27%), phosphofructokinase (-20%) and Mg2+- and Ca2+-ATPase activity of purified myofibrils (-33%, -44%). Exogenous thyroxin replacement restored those parameters studied to normal with the exception that it persistently elevated citrate synthase activity significantly above normal control levels. Responses similar to those of Td neonates occurred when adult rats were similarly treated. Sx produced no consistent effects on respiratory and glycogenolytic marker enzymes, but caused a 20% reduction in Ca2+-ATPase activity of both neonatal and adult cardiac myofibrils. These findings suggest that cardiac muscle cells require thyroxin for normal growth and enzyme development. Also, Sx may impair cardiac functional capacity by altering Ca2+ activity of actomyosin ATPase.
D000818:Animals; D001835:Body Weight; D000252:Calcium-Transporting ATPases / Q000378:metabolism; D002950:Citrate (si)-Synthase / Q000378:metabolism; D003574:Cytochrome c Group / Q000378:metabolism; D006321:Heart / Q000294:innervation; D009206:Myocardium / Q000201:enzymology*; D009210:Myofibrils / Q000201:enzymology; D010732:Phosphofructokinase-1 / Q000378:metabolism; D051381:Rats; D013564:Sympathetic Nervous System / Q000502:physiology*; D013961:Thyroid Gland / Q000502:physiology*; D013974:Thyroxine / Q000494:pharmacology
D003574:Cytochrome c Group; D002950:Citrate (si)-Synthase; D010732:Phosphofructokinase-1; D000252:Calcium-Transporting ATPases; D013974:Thyroxine