Characterization of a colchicine receptor protein in rat epididymal adipose tissue.
Colchincine was found to be taken up by adipose tissue and therein to bind to a soluble macromolecule not sedimented by centrifugation for 2 h at 100 000 x g. A similar binding occurred when soluble extracts of adipose tissue were incubated with colchicine. The binding reaction reaction is temperature dependent and shows a pH optimum between 6.8 and 7.0. Double reciprocal plots of colchicine concentration versus amounts of colchicine bound to protein in the steady state disclosed an apparent Km of 0.250 to 1.5 muM. The colchicine binding activity of soluble tissue extracts decreased when the extracts were incubated at 37 degree C. Addition of guanosine triphosphate and Mg-2+ retarded the loss of colchicine binding activity. The molecular weight of the colchicine complex was estimated to be 115 000 and its sedimentation coefficient 5.8 S. All of these characteristics are remarkably similar to those of the protein tubulin which has been isolated from other tissues. Since it is now well known that tubulin is a protein subunit of cytoplasmic microtubules, it is suggested that the previously reported metabolic effects of colchicine on adipose tissue result from the dissolution of microtubules by colchicine.
D000273:Adipose Tissue / Q000032:analysis* / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D002458:Cell Fractionation; D002852:Chromatography, Ion Exchange; D003078:Colchicine / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D004822:Epididymis; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008297:Male; D008861:Microsomes / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D008970:Molecular Weight; D011485:Protein Binding; D011506:Proteins / Q000032:analysis; D051381:Rats; D011955:Receptors, Drug; D013997:Time Factors; D014280:Triglycerides / Q000378:metabolism; D014461:Ultracentrifugation
D011506:Proteins; D011955:Receptors, Drug; D014280:Triglycerides; D003078:Colchicine