Polyamines and cellular adenosine 3' :5'-cyclic monophosphate.
The effect of polyamines on the cellular concentrations of cyclic AMP was studied. It was shown that 1 microM-spermine caused a decrease in cyclic AMP in chick-embryo heart cells, chick-embryo fibroblasts, neuroblastoma, glioma and neuroblastoma-glioma hybrid cells, grown in culture. A similar decrease was observed when polyamines were added to cells in the presence of a phosphodiesterase inhibitor or after stimulating the cells with various hormones. Noradrenaline was used in cultures of heart cells, prostaglandin E1 and adenosine for neuroblastoma and neuroblastoma-glioma hybrids, whereas isoproterenol was used for the stimulation of glioma cells. Polyamines at higher concentrations were either without effect or caused a slight increase in cyclic AMP. Spermidine (10 microM) also caused a decrease in cellular cyclic AMP, as did 0.1 microM-putrescine. It is suggested that the effect of polyamines on cellular cyclic AMP may be explained by the effect of these polycations on the activity of cellular phosphodiesterase.
D000241:Adenosine / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D000242:Cyclic AMP / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D005910:Glioma / Q000378:metabolism; D006822:Hybrid Cells / Q000378:metabolism; D009206:Myocardium / Q000166:cytology / Q000378:metabolism; D009447:Neuroblastoma / Q000378:metabolism; D009638:Norepinephrine / Q000494:pharmacology; D011073:Polyamines / Q000494:pharmacology*; D051381:Rats
D011073:Polyamines; D000242:Cyclic AMP; D000241:Adenosine; D009638:Norepinephrine