Determination of the length distribution of poly(A) at the 3' terminus of the virion RNAs of EMC virus, poliovirus, rhinovirus, RAV-61 and CPMV and of mouse globin mRNA.
Rapid, detailed, and accurate analysis of the length spectrum of 3' terminal poly(A) in an RNA population can be obtained by 3'-terminal 32P-labelling of RNA with T4 RNA ligase, digestion with ribonucleases T1 and A, and use of gel sequencing methods. Length distributions of 3'-terminal poly(A) of EMC virus, poliovirus, rhinovirus, RAV-61, and CPMV virion RNAs as well as mouse globin mRNA are presented.
D000818:Animals; D001483:Base Sequence; D004686:Encephalomyocarditis virus / Q000032:analysis*; D005914:Globins / Q000096:biosynthesis*; D051379:Mice; D008970:Molecular Weight; D009029:Mosaic Viruses / Q000032:analysis*; D017955:Poliovirus / Q000032:analysis*; D011061:Poly A / Q000032:analysis*; D012333:RNA, Messenger; D012367:RNA, Viral; D012229:Rhinovirus / Q000032:analysis*; D006163:Ribonuclease T1; D012260:Ribonucleases; D013045:Species Specificity
D012333:RNA, Messenger; D012367:RNA, Viral; D011061:Poly A; D005914:Globins; D012260:Ribonucleases; D006163:Ribonuclease T1