Preparation of inactivated oil-emulsion vaccines with avian viral or Mycoplasma antigens.
The influence of the composition of water-in-oil emulsions on their physical characteristics was determined by preparing experimental emulsions with various water-to-oil ratios and various emulsifiers. Emulsions containing Tween 80 in the aqueous phase and Arlacel A or Arlacel 80 in the oil phase were lower in viscosity than emulsions containing only an oil-phase emulsifier. Viscosity decreased as the concentration of oil increased. Oil-emulsion vaccines prepared with aqueous- and oil-phase emulsifiers had low viscosity, were stable for more than 12 weeks at 37 C, and induced a marked primary antibody response in chickens.
D000818:Animals; D000917:Antibody Formation; D001428:Bacterial Vaccines / Q000008:administration & dosage; D002645:Chickens / Q000276:immunology; D004655:Emulsions; D001351:Infectious bronchitis virus / Q000276:immunology; D009980:Influenza A virus / Q000276:immunology; D008722:Methods; D008899:Mineral Oil; D009174:Mycoplasma / Q000276:immunology*; D009522:Newcastle disease virus / Q000276:immunology; D012328:RNA Viruses / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage; D014783:Viscosity
D001428:Bacterial Vaccines; D004655:Emulsions; D014765:Viral Vaccines; D008899:Mineral Oil