title: A cleavage map of the TOL plasmid of Pseudomonas putida mt-2.
abstract: A cleavage map of the TOL plasmid pWWO has been determined for the restriction endonucleases HindIII and XhoI. A number of techniques were employed including (i) digestion of purified cleavage products with a second enzyme; (ii) hybridisation of purified XhoI fragments to Southern blots of HindIII digest products and (iii) analysis of a number of deletion mutants.
mesh_terms: D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial / Q000648:ultrastructure*; D004262:DNA Restriction Enzymes; D010957:Plasmids; D011549:Pseudomonas / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D014050:Toluene / Q000378:metabolism; D014992:Xylenes / Q000378:metabolism
chemical_list: D014992:Xylenes; D014050:Toluene; D004262:DNA Restriction Enzymes