Effects of an acetyl-coenzyme A carboxylase inhibitor and a sodium-sparing diuretic on aldosterone-stimulated sodium transport, lipid synthesis, and phospholipid fatty acid composition in the toad urinary bladder.
A correlation study of the effects of two agents, 2-methyl-2-[p-(1,2,3,4-tetrahydro-1-naphthyl)phenoxy]propionic acid (TPIA) and amiloride, on aldosterone-induced alterations in Na+ transport, lipid synthesis, and phospholipid fatty acid composition has been carried out in the toad urinary bladder. TPIA, an inhibitor of acetyl-CoA carboxylase, inhibits aldosterone-stimulated Na+ transport as well as hormone-induced lipid synthesis and the increase in weight percentage of phospholipid long-chain polyunsaturated fatty acids. Amiloride, a diuretic which blocks sodium entry into the transporting epithelium, does not alter aldosterone's effects on lipid and fatty acid metabolism but prevents the hormone-induced increase in Na+ transport. These results support the conclusion that aldosterone increases Na+ transport in the toad urinary bladder by altering membrane fatty acid metabolism and that the lipid biosynthetic events following aldosterone treatment are a primary response to the hormone and not secondary to increased Na+ transport.
D000085:Acetates / Q000378:metabolism; D000103:Acetyl-CoA Carboxylase / Q000032:analysis*; D000450:Aldosterone / Q000494:pharmacology*; D000584:Amiloride / Q000494:pharmacology*; D000818:Animals; D001693:Biological Transport, Active; D002024:Bufo marinus; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D007930:Leucine / Q000378:metabolism; D008025:Ligases / Q000032:analysis*; D008055:Lipids / Q000096:biosynthesis*; D009255:Nafenopin / Q000494:pharmacology*; D010743:Phospholipids / Q000378:metabolism; D011422:Propionates / Q000494:pharmacology*; D014176:Protein Biosynthesis; D011719:Pyrazines / Q000494:pharmacology*; D012313:RNA / Q000096:biosynthesis; D012964:Sodium / Q000378:metabolism*; D014529:Uridine / Q000378:metabolism; D001743:Urinary Bladder / Q000187:drug effects / Q000378:metabolism*
D000085:Acetates; D005227:Fatty Acids; D008055:Lipids; D010743:Phospholipids; D011422:Propionates; D011719:Pyrazines; D009255:Nafenopin; D000450:Aldosterone; D012313:RNA; D000584:Amiloride; D012964:Sodium; D008025:Ligases; D000103:Acetyl-CoA Carboxylase; D007930:Leucine; D014529:Uridine