Purification, crystallization, and properties of D-ribose isomerase from Mycobacterium smegmatis.
D-Ribose isomerase, which catalyzes the conversion of D-ribose to D-ribulose, was purified from extracts of Mycobacterium smegmatis grown on D-ribose. The purified enzyme crystalized as hexagonal plates from a 44% solution of ammonium sulfate. The enzyme was homogenous by disc gel electrophoresis and ultracentrifugal analysis. The molecular weight of the enzyme was between 145,000 and 174,000 by sedimentation equilibrium analysis. Its sedimentation constant of 8.7 S indicates it is globular. On the basis of sodium dodecyl sulfate gel electrophoresis in the presence of Mn2+, the enzyme is probably composed of 4 identical subunits of molecular weight about 42,000 to 44,000. The enzyme was specific for sugars having the same configuration as D-ribose at carbon atoms 1 to 3. Thus, the enzyme could also utilize L-lyxose, D-allose, and L-rhamnose as substrates. The Km for D-ribose was 4 mM and for L-lyxose it was 5.3 mM. The enzyme required a divalent cation for activity with optimum activity being shown with Mn2+. the Km for the various cations was as follows: Mn2+, 1 times 10(-7) M, Co2+, 4 times 10(-7) M, and Mg2+, 1.8 times 10(-5) M. The pH optimum for the enzyme was 7.5 to 8.5. Polyols did not inhibit the enzyme to any great extent. The product of the reaction was identified as D-ribulose by thin layer chromatography and by preparation of the O-nitrophenylhydrazone derivative.
D019747:Aldose-Ketose Isomerases; D002238:Carbohydrate Epimerases / Q000302:isolation & purification / Q000378:metabolism*; D003460:Crystallization; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009161:Mycobacterium / Q000201:enzymology*; D012266:Ribose / Q000378:metabolism; D013329:Structure-Activity Relationship
D012266:Ribose; D002238:Carbohydrate Epimerases; D019747:Aldose-Ketose Isomerases; C022546:ribose isomerase