Relationship between 5'-nucleotidase, adenosine deaminase, AMP deaminase, ATP-(Mg2+)-ase activities and dTMP kinase activity in rat liver mitochondria.
The activities of dTMP kinase (ATP-deoxythymidine monophosphate phosphotransferase, EC 2.7.4.9), 5'-nucleotidase (5'-ribonucleoside phosphohydrolase, EC 3.1.3.5), adenosine deaminase (adenosine aminohydrolase, EC 3.5.4.4), AMP deaminase (AMP aminohydrolase, EC 3.5.3.6) and ATP-(Mg2+)-ase (ATP phosphohydrolase, EC 3.6.1.3) were assayed in mitochondria of normal and regenerating rat liver. In regenerating mitochondria, the dTMP kinase activity increased 20 times, 5'-nucleotidase (5'Nase) activity for dTMP diminished by 65% and its activity for other nucleoside monophosphates did not change; adenosine deaminase activity for adenosine (AR) increased by 40%, but for deoxyadenosine (AdR) decreased by 70%. AMP deaminase and ATP-(Mg2+)-ase activities behaved similarly in mitochondria from regenerating liver, decreasing by 70 and 64% respectively. The changes of the amount of dTMP in mitochondria depend on enzyme activities which regulate the AdR concentration.
D000659:AMP Deaminase / Q000378:metabolism*; D000241:Adenosine / Q000494:pharmacology; D000243:Adenosine Deaminase / Q000378:metabolism*; D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D003839:Deoxyadenosines / Q000494:pharmacology; D005260:Female; D006498:Hepatectomy; D007425:Intracellular Membranes / Q000201:enzymology; D008115:Liver Regeneration; D008274:Magnesium / Q000494:pharmacology; D008930:Mitochondria, Liver / Q000201:enzymology*; D009700:Nucleoside Deaminases / Q000378:metabolism*; D009703:Nucleoside-Phosphate Kinase / Q000378:metabolism*; D009708:Nucleotidases / Q000378:metabolism*; D009709:Nucleotide Deaminases / Q000378:metabolism*; D010770:Phosphotransferases / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000494:pharmacology; D051381:Rats; D013938:Thymidine Monophosphate / Q000494:pharmacology
D003839:Deoxyadenosines; D013938:Thymidine Monophosphate; D011092:Polyethylene Glycols; D010770:Phosphotransferases; D009703:Nucleoside-Phosphate Kinase; C020093:dTMP kinase; D009708:Nucleotidases; D009700:Nucleoside Deaminases; D009709:Nucleotide Deaminases; D000243:Adenosine Deaminase; D000659:AMP Deaminase; D000251:Adenosine Triphosphatases; D008274:Magnesium; D000241:Adenosine