title: In vivo suppression of allograft rejection by cyclic AMP increasing agents.
abstract: This study was undertaken to elucidate the possibilities of modulating the survival of allogeneic tissue grafts by administration of cyclic AMP-increasing agents to the graft recipients. Heterotopic grafting of split mouse hearts across a strong H-2 barrier was used. It was found that Vibrio cholerae enterotoxin, which activates adenylate cyclase in mammalian cells, gave a marked prolongation of the graft survival time when given shortly before (but not after) transplantation. Also the administration of a combination of cyclic AMP and theophylline prior to or around transplantation increased graft survival time significantly. In contrast, no effect was obtained with choleragenoid, a membrane-binding cholera toxin analogue devoid of cyclic AMP-increasing activity.
mesh_terms: D000818:Animals; D002772:Cholera Toxin / Q000494:pharmacology; D000242:Cyclic AMP / Q000096:biosynthesis*; D006084:Graft Rejection; D016027:Heart Transplantation; D007165:Immunosuppression Therapy; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D013806:Theophylline / Q000494:pharmacology; D014184:Transplantation, Homologous
chemical_list: D002772:Cholera Toxin; D013806:Theophylline; D000242:Cyclic AMP