Electron transport systems of lung microsomes and their physiological functions. Enzymic hydroxylation of aniline and steroids.
1. The hydroxylation of aniline by rabbit lung microsomes to rho-aminophenol required oxygen and NADPH, and was inhibited by menadione, ferricytochrome c and carbon monoxide. 2. NADH was a less effective electron donor than NADPH in this reaction, but its addition significantly increased the yield of rho-aminophenol formed in the presence of NADPH. 3. 4,16-Androstadien-3-one and 3beta-hydroxy-5-androsten-17-one were also metabolized by lung microsomes to the same products as are formed by hepatic microsomes.
D000730:Androstadienes / Q000378:metabolism; D000737:Androstenols / Q000378:metabolism; D000814:Aniline Compounds / Q000378:metabolism*; D000818:Animals; D002248:Carbon Monoxide / Q000494:pharmacology; D002474:Cell-Free System; D002855:Chromatography, Thin Layer; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D003574:Cytochrome c Group / Q000494:pharmacology; D004579:Electron Transport; D006900:Hydroxylation; D008168:Lung / Q000378:metabolism*; D008297:Male; D008861:Microsomes / Q000201:enzymology / Q000378:metabolism*; D008862:Microsomes, Liver / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D010100:Oxygen; D010636:Phenols / Q000096:biosynthesis; D011817:Rabbits; D013256:Steroids / Q000378:metabolism*; D013347:Subcellular Fractions; D014812:Vitamin K / Q000494:pharmacology
D000730:Androstadienes; D000737:Androstenols; D000814:Aniline Compounds; D003574:Cytochrome c Group; D010636:Phenols; D013256:Steroids; D014812:Vitamin K; D009249:NADP; D002248:Carbon Monoxide; D003577:Cytochrome P-450 Enzyme System; D009251:NADPH-Ferrihemoprotein Reductase; D010100:Oxygen