Hapten inhibition of adsorption: specificity of the Sophora japonica lectin.
Since hapten inhibition of precipitation is relatively time consuming, we have developed a hapten inhibition of adsorption assay to explore the sugar binding specificity of the Sophora japonica lectin. Adsorbents were prepared with Sephadex and p-aminophenyl beta-D-galactopyranoside using the CNBr procedure. The ability of simple saccharides to inhibit the binding of lectin to the adsorbent was performed employing a fixed amount of adsorption with respect to the logarithm of micromoles of inhibitor yielded sigmoid shaped curves. The quantities of various saccharides required to cause 50% inhibition of the lectin--adsorbent interaction relative to D-galactose were within+/-5% of the relative inhibiting potencies of the sugars in
D000017:ABO Blood-Group System; D000327:Adsorption; D000814:Aniline Compounds; D000918:Antibody Specificity; D002241:Carbohydrates; D003488:Cyanogen Bromide; D003911:Dextrans; D005690:Galactose; D006027:Glycosides; D006241:Haptens; D006863:Hydrogen-Ion Concentration; D037102:Lectins
D000017:ABO Blood-Group System; D000814:Aniline Compounds; D002241:Carbohydrates; D003911:Dextrans; D006027:Glycosides; D006241:Haptens; D037102:Lectins; D003488:Cyanogen Bromide; D005690:Galactose