Studies on the interaction of concanavalin A with glycoproteins.
Lectins (phytohaemagglutinin) are known to have the unique property of binding with certain specific sugars, polysaccharides and glycoproteins. Although the kinetics of interaction between lectins and sugar have been extensively studied, the binding characteristics of the lectins with various glycoproteins are not well understood. In this laboratory a systematic study has been initiated in relation to the interaction of lectins with glycoproteins. Concanavalin A is known to bind alpha-glucosides, mannosides and biopolymers having these sugar configurations. A galactose binding protein from caster bean has been purified to homogeneity and was found to contain mannose. This lectin was used as the source of glycoprotein for studying its interaction with concanavalin A. This study showed that the interaction is temperature dependent and the dissociation is time and alpha-methyl glucoside concentration dependent. This has led to speculate a model for cell-lectin interaction. Using concanavalin A it has been shown that all the lysosomal enzymes from brain studied were glycoprotein in nature. Moreover, using Sepharose-bound concanavalin A it has been possible to devise a method by which these lysosomal enzymes could be purified considerably. With the knowledge that the interaction between lectin and glycoprotein is not only dependent on the specific sugar present in the glycoprotein, but also on the nature of the glycoprotein it was possible to develop a novel method for immobilizing various glycoprotein enzymes, such as arylsulphatase A, hyaluronidase and glucose oxidase.
D000818:Animals; D001192:Arylsulfatases / Q000302:isolation & purification; D001921:Brain / Q000201:enzymology; D002241:Carbohydrates / Q000302:isolation & purification; D055598:Chemical Phenomena; D002621:Chemistry; D002645:Chickens; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D003208:Concanavalin A; D004586:Electrophoresis; D005949:Glucose Oxidase / Q000302:isolation & purification; D006023:Glycoproteins / Q000302:isolation & purification; D006821:Hyaluronoglucosaminidase / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000032:analysis; D007700:Kinetics; D037102:Lectins; D008247:Lysosomes / Q000201:enzymology; D008362:Mannosides; D011485:Protein Binding
D002241:Carbohydrates; D006023:Glycoproteins; D007527:Isoenzymes; D037102:Lectins; D008362:Mannosides; D003208:Concanavalin A; D005949:Glucose Oxidase; D001192:Arylsulfatases; D006821:Hyaluronoglucosaminidase