title: Structural organization of human cytomegalovirus DNA.
abstract: HCMV DNA preparations in which purity was characterized by sedimentation, buoyant density, and restriction enzyme cleavage contain two molecular weight size classes of HCMV DNA molecules (150--155 x 10(6) and 100 x 10(6) daltons) in addition to some shear products. Partial denaturation mapping reveals that both size classes contain the same sequences and that molecules of 150--155 x 10(6) daltons contain the apparent full complement of sequences which are variously contained in the smaller molecules. The complete genome (150--155 x 10(6) daltons) seems to be divisible into regions of about 78--82% and 18--22%, which undergo complete inversions with respect to one another. Enriched or purified preparations of molecules of 150--155 x 10(6) daltons generate cleavage products identical to those from total HCMV DNA preparations.
mesh_terms: D001483:Base Sequence; D002499:Centrifugation, Density Gradient; D002874:Chromosome Mapping; D003587:Cytomegalovirus / Q000235:genetics / Q000378:metabolism*; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D005814:Genes, Viral; D008970:Molecular Weight; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization
chemical_list: D004279:DNA, Viral; D004262:DNA Restriction Enzymes