Preparation and efficacy of an inactivated subunit vaccine (NFUIBHK) against type 2 Herpes simplex virus infection.
A vaccine against Herpes simplex virus infection was prepared by Nonidet NP 40 and formalin treatment of a type 1, infected-cell extract; virus particles were removed by ultracentrifugation over sucrose. These procedures were not detrimental to the antigenic quality of the vaccine preparation. The vaccine afforded significant protection to experimental type 2 genital herpes virus infection in mice, as adjudged by clinical observations, cytopathological change, and virus yields.
D000818:Animals; D000956:Antigens, Viral / Q000302:isolation & purification; D002460:Cell Line; D005260:Female; D005557:Formaldehyde / Q000494:pharmacology; D005831:Genital Diseases, Female / Q000517:prevention & control*; D006561:Herpes Simplex / Q000517:prevention & control*; D006801:Humans; D051379:Mice; D018139:Simplexvirus / Q000187:drug effects / Q000276:immunology*; D013501:Surface-Active Agents / Q000494:pharmacology; D014461:Ultracentrifugation; D014765:Viral Vaccines / Q000276:immunology / Q000302:isolation & purification
D000956:Antigens, Viral; D013501:Surface-Active Agents; D014765:Viral Vaccines; D005557:Formaldehyde