Metabolism of prostaglandins E, A, and C in serum.
Three prostaglandin-metabolizing enzymes were detected in human serum. One enzyme was a dehydrase that converted prostaglandin E to prostaglandin A, the second was a prostaglandin A isomerase that converted prostaglandin A to prostaglandin C and the third was a prostaglandin C isomerase that converted prostaglandin C to prostaglandin B. All three were inactivated by sulfhydryl blocking agents. In human serum, only prostaglandin C isomerase had high activity, whereas the two other enzymes had very low activity. In rabbit serum both isomerases were very active, but dehydrase activity could not be detected. Prostaglandin C isomerase activity was also found in crystallized human serum albumin and rabbit serum albumin.
D000818:Animals; D005227:Fatty Acids / Q000097:blood; D006801:Humans; D006836:Hydro-Lyases / Q000097:blood; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D007535:Isomerases / Q000097:blood; D011453:Prostaglandins / Q000097:blood*; D011817:Rabbits; D011863:Radioimmunoassay; D012709:Serum Albumin; D013056:Spectrophotometry, Ultraviolet
D005227:Fatty Acids; D011453:Prostaglandins; D012709:Serum Albumin; D006836:Hydro-Lyases; D007535:Isomerases