A new and rapid system for the determination of enzymatic activity of bacterial collagenases.
Collagenase activity is assayed using N-CBZ-Gly-Pro-Gly-Pro-Ala as substrate in an autoanalyzer II system. The liberation of the tripeptide is monitored after the reaction with trinitrobenzenesulfonic acid. The new system shows a considerably improved precision when compared to existing methods and allows about ten times as many samples to be analyzed per day. This is of importance for laboratories where large amounts of activity determinations have to be performed.
D001322:Autoanalysis / Q000295:instrumentation; D001419:Bacteria / Q000201:enzymology*; D008722:Methods; D003012:Microbial Collagenase / Q000378:metabolism*; D009842:Oligopeptides
D009842:Oligopeptides; D003012:Microbial Collagenase