Induction of T-cells differentiation in vitro by thymus epithelial cells.
Thymus-reticular epithelial cells (TE-cells) were grown in a cell culture devoid of any lymphocytic elements. These cells were able to induce T-cell differentiation in spleen cells from T-dificient mice as expressed by con-A responsiveness and GvH reactivity. It was also shown that xenogeneic rat TE cells were as effective in the induction of T-cell differentiation in vitro as syngeneic TE cells. This system is therefore ideal for the study of T-cell development.
D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D003208:Concanavalin A; D004247:DNA / Q000096:biosynthesis; D004847:Epithelial Cells; D004848:Epithelium / Q000276:immunology* / Q000648:ultrastructure; D005260:Female; D006087:Graft vs Host Reaction; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D008854:Microscopy, Electron; D011828:Radiation Chimera; D051381:Rats; D011917:Rats, Inbred Lew; D013045:Species Specificity; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology* / Q000637:transplantation; D013934:Thymectomy; D013936:Thymidine / Q000378:metabolism; D013950:Thymus Gland / Q000166:cytology* / Q000276:immunology; D014184:Transplantation, Homologous; D014316:Tritium
D014316:Tritium; D003208:Concanavalin A; D004247:DNA; D013936:Thymidine