Survival and herpes virus production of normal and xeroderma pigmentosum fibroblasts after treatment with formaldehyde.
The survival of excision-deficient and of excision-proficient (variant) skin fibroblasts from xeroderma pigmentosum (XP) donors was about 5 times and twice, respectively, more sensitive to formaldehyde (FA) treatment than that of skin fibroblasts from healthy and XP heterozygote donors. The capacity of FA-treated host cells to further support Herpes virus (HSV) replication was also more sensitive to FA in XP12BE (group A) than in normal (KD) cells. An important recovery of this capacity occurred in both cell types when they were infected at increasing times (up to 36 h) after FA treatment. This contrasts with the decreasing capacity observed in XP12BE when similarly infected at increasing times after exposure to ultraviolet. In addition, the survival of FA-treated HSV was comparable in KD and XP12BE cells, whereas that of UV-irradiated HSV was much lower in XP12BE than in KD cells.
D002460:Cell Line; D002478:Cells, Cultured; D004260:DNA Repair; D005557:Formaldehyde / Q000494:pharmacology*; D006801:Humans; D010641:Phenotype; D018139:Simplexvirus / Q000254:growth & development*; D012867:Skin / Q000378:metabolism*; D014779:Virus Replication / Q000187:drug effects*; D014983:Xeroderma Pigmentosum / Q000235:genetics*
D005557:Formaldehyde