A method for observing protein-protein interaction.
A method is proposed for observation of the interaction between charged macromolecules such as proteins. The method is based on the fact that the pK of an ionizable reporter group attached to a macromolecule can be altered by the electrostatic effect of another charged macromolecule which associates with the former. The effectiveness of the method was shown in the study of the association of bovine serum albumin with hen egg lysozyme [EC 3.2.1.17]. The  errors inherent in this method in obtaining the equilibrium constant of the association reaction and procedures for their correction are discussed.
D000818:Animals; D004527:Egg Proteins / Q000032:analysis; D004563:Electrochemistry; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D046911:Macromolecular Substances; D008722:Methods; D009113:Muramidase / Q000032:analysis; D011506:Proteins / Q000032:analysis; D011522:Protons; D012710:Serum Albumin, Bovine / Q000032:analysis; D013053:Spectrophotometry; D014461:Ultracentrifugation
D004527:Egg Proteins; D046911:Macromolecular Substances; D011506:Proteins; D011522:Protons; D012710:Serum Albumin, Bovine; D009113:Muramidase