Continuous-flow analysis of ammonia in perchloric acid supernate of blood or plasma using an ammonia-selective electrode.
A simple automated method for the estimation of ammonia in perchloric acid supernate of blood or plasma using an ion-selective electrode (Orion Ammonia-selective electrode, Model 95-10) is described. The reliability of the proposed method has been checked against an ion-exchange resin procedure, which has been chosen as a standard procedure. Regression equation and correlation coefficient for the proposed method are y = 0.7 X + 10 and 0.945, respectively, as compared with the chosen standard method. Within-run and between-run precision are 2.1% and 3.5% respectively. The average percent recovery is 97.5% and a tentative range is 13-73 microgram/dl (9-52 micronmol/l) ammonia nitrogen.
D000328:Adult; D000641:Ammonia / Q000097:blood*; D001322:Autoanalysis; D004566:Electrodes; D005260:Female; D006801:Humans; D008722:Methods; D010472:Perchlorates; D011897:Random Allocation; D013997:Time Factors
D010472:Perchlorates; D000641:Ammonia