Action of rennin on kappa-casein.
The action of rennin on kappa-casein was studied as a function of time using several methods to measure activity. The first indication of rennin cleavage of kappa-casein is precipitability in .1 M acetate buffer at pH 5.2 and 5 C. A longer exposure to rennin is required to alter kappa-casein so that it forms a precipitate with calcium ions and loses its ability to stabilize alpha s-casein. The least sensitive indication of rennin activity is measurement of nitrogen soluble in 2% trichloroacetic acid. Electrophoresis experiments showed that these methods detect various stages in the conversion of kappa-casein para-kappa-casein.
D002122:Calcium Chloride; D002364:Caseins; D002498:Centrifugation; D055598:Chemical Phenomena; D011232:Chemical Precipitation; D002621:Chemistry; D012085:Chymosin; D004591:Electrophoresis, Polyacrylamide Gel; D006863:Hydrogen-Ion Concentration; D009584:Nitrogen / Q000032:analysis; D013696:Temperature; D013997:Time Factors; D014238:Trichloroacetic Acid
D002364:Caseins; D014238:Trichloroacetic Acid; D012085:Chymosin; D002122:Calcium Chloride; D009584:Nitrogen