title: Effects of several preoperative medications on fat cell lipolysis, and activity of adipose tissue cyclic AMP phosphodiesterase.
abstract: Effects were examined of atropine, diazepam, pethidene, promethazine, scopolamine, omnopon and papaverine on basal and noradrenaline-stimulated lipolysis in rat isolated fat cells and on rat adipose tissue cyclic AMP phosphodiesterase activity. Papaverine at high concentration (1 mM) inhibited both basal and hormone-stimulated lipolysis, whereas diazepam enhanced basal lipolysis. At a 'clinical dose', omnopon increased both basal and noradrenaline-stimulated lipolysis. Adipose tissue cAMP phosphodiesterase activity was strongly inhibited by 1 mM diazepam, papaverine, promethazine and omnopon (280 microgram ml-1). Lack of enhancement of lipolysis by the established cAMP phosphodiesterase antagonist papaverine, is compatible with simultaneous inhibition also of adipose adenyl cyclase. Diazepam-stimulated lipolysis is compatible with its phosphodiesterase inhibitory activity. It is proposed that papaverine-containing omnopon may offer some survival advantages during surgical stress by facilitating a caloric supply.
mesh_terms: D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000378:metabolism*; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D003975:Diazepam / Q000494:pharmacology; D008053:Lipid Mobilization / Q000187:drug effects*; D008297:Male; D008614:Meperidine / Q000494:pharmacology; D010208:Papaverine / Q000494:pharmacology; D011398:Promethazine / Q000494:pharmacology; D051381:Rats; D012601:Scopolamine / Q000494:pharmacology
chemical_list: D001285:Atropine; D008614:Meperidine; D010208:Papaverine; D012601:Scopolamine; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D011398:Promethazine; D003975:Diazepam