Multilayer planar membranes of sarcoplasmic reticulum.
Multilayer planar membranes were constructed between a pair of cellulose sheets from fragmented sarcoplasmic reticulum (FSR) as well as a mixture of egg yolk lecithin and the Ca2+-ATPase purified from FSR. Since sodium deoxycholate was used instead of organic solvents in order to dissolve phospholipids in the process of the membrane preparation, the total activity of the Ca2+-ATPase was still preserved in the planar membrane of FSR. It was also indicated using a spin label technique that the orientation of phospholipids in the planar membrane of FSR was considerably disturbed by the presence of proteins such as the Ca2+-ATPase included in FSR.
D000818:Animals; D000252:Calcium-Transporting ATPases / Q000032:analysis; D002482:Cellulose; D004578:Electron Spin Resonance Spectroscopy; D008567:Membranes, Artificial; D010713:Phosphatidylcholines; D010743:Phospholipids; D012519:Sarcoplasmic Reticulum / Q000201:enzymology / Q000648:ultrastructure*
D008567:Membranes, Artificial; D010713:Phosphatidylcholines; D010743:Phospholipids; D002482:Cellulose; D000252:Calcium-Transporting ATPases