Prostaglandin-E2-9-ketoreductase in rabbit kidney.
The 100,000 xg supernatant of rabbit kidney contains a prostaglandin-E2-9-ketoreductase which has an obligatory requirement for NADPH. This enzyme is localised in the renal cortex and is able to quantitatively convert PGE2 to PGF2alpha. A broad pH profile was evident with an optimum at pH 7 with 5. Kinetic studies indicated a Km of 3 with 2 x 10-4M PGE2. The isoelectric point was at pH 5 with 65 and the molecular weight, as estimated by gel filtration, was 21,800. These values differ from those obtained with enzyme from monkey brain tissue and suggest a tissue specificity of PGE2-9-ketoreductase. By combining isoelectric focussing techniques with sephadex filtration considerable purification of the renal enzyme was achieved.
D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000201:enzymology; D002850:Chromatography, Gel; D005260:Female; D000882:Haplorhini; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D007668:Kidney / Q000201:enzymology*; D007700:Kinetics; D008253:Macaca mulatta; D008970:Molecular Weight; D009249:NADP / Q000378:metabolism; D010088:Oxidoreductases / Q000302:isolation & purification; D011453:Prostaglandins; D011454:Prostaglandins A / Q000378:metabolism; D011458:Prostaglandins E; D011460:Prostaglandins F / Q000378:metabolism; D011817:Rabbits; D011863:Radioimmunoassay; D013997:Time Factors
D011453:Prostaglandins; D011454:Prostaglandins A; D011458:Prostaglandins E; D011460:Prostaglandins F; D009249:NADP; D010088:Oxidoreductases; D000429:Alcohol Oxidoreductases