Electrophoretic heterogeneity of 5-phosphoribosyl-1-pyrophosphate synthetase within and among humans.
The product of the enzyme 5-phosphoribosyl-1-pyrophosphate (PPriboseP) synthetase is a substrate for purine, pyrimidine, and pyridine biosynthesis and may be rate limiting for purine biosynthesis. A system developed to electrophoretically separate and histochemically detect PPriboseP synthetase in crude cell extracts has facilitated the identification of electrophoretically variant enzyme forms in the erythrocytes of five patients from a patient population of 200. Additional studies of human organs obtained at autopsy revealed a unique electrophoretic mobility for nearly each organ within the same individual.
D004588:Electrophoresis, Cellulose Acetate; D004912:Erythrocytes / Q000201:enzymology; D014644:Genetic Variation; D006801:Humans; D010770:Phosphotransferases / Q000235:genetics*; D012268:Ribose-Phosphate Pyrophosphokinase / Q000235:genetics*; D014018:Tissue Distribution
D010770:Phosphotransferases; D012268:Ribose-Phosphate Pyrophosphokinase