A new method for the assay of aminoacylase: elaboration of a fixed-incubation method for routine measurements.
A simple method suitable for routine determinations of aminoacylase (EC 3.5.1.14) in serum and tissue homogenates is described. It is based on the formation of red charge-transfer complexes from p-benzoquinone and amino acids liberated by cleavage of acylamino acids. Optimal substrates are trifluoroacetyl-L-methionine and chloroacetyl-L-methionine, the first being rapidly hydrolyzed by pancreatic tissue, the latter by liver and kidney homogenates. Sera preferentially split the chloroacetyl compound. Optimal conditions for the assay of serum activity are: 18-20 mm9l/l substrate concentration, 50 mmol/l phosphate buffer pH 6.0, no additives.
D000581:Amidohydrolases / Q000032:analysis* / Q000097:blood; D000596:Amino Acids; D002413:Cations, Divalent; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D008715:Methionine; D009362:Neoplasm Metastasis; D009928:Organ Specificity; D010179:Pancreas / Q000201:enzymology; D013053:Spectrophotometry / Q000379:methods
D000596:Amino Acids; D002413:Cations, Divalent; D008715:Methionine; D000581:Amidohydrolases; C019672:aminoacylase I