The p-hydroxylation of amphetamine and phentermine by rat liver microsomes.
1. The products of p-hydroxylation of amphetamine and phentermine by two different preparations of rat liver microsomes were identified and quantitatively determined. At low concentrations (muM) significant proportions of the substrates were metabolized to the p-hydroxy derivatives by an NADPH-dependent system. The enzyme system was inhibited by higher substrate concentrations (mM) and was not induced by either phenobarbital or 3-methylcholanthrene. 2. The properties of this in vitro system are consistent with reports on in vivo studies of this reaction.
D000661:Amphetamine / Q000378:metabolism*; D000815:Aniline Hydroxylase / Q000378:metabolism; D000818:Animals; D004790:Enzyme Induction / Q000187:drug effects; D008401:Gas Chromatography-Mass Spectrometry; D008297:Male; D008748:Methylcholanthrene / Q000494:pharmacology; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology*; D006899:Mixed Function Oxygenases / Q000378:metabolism; D009249:NADP; D010634:Phenobarbital / Q000494:pharmacology; D010645:Phentermine / Q000378:metabolism*; D051381:Rats
D009249:NADP; D008748:Methylcholanthrene; D010645:Phentermine; D000661:Amphetamine; D006899:Mixed Function Oxygenases; D000815:Aniline Hydroxylase; D010634:Phenobarbital