Regulation of cytoplasmic dihydrotestosterone binding in dog prostate by 17 beta-estradiol.
17 beta-estradiol enchances androgen-induced prostate growth in the castrate dog to a degree comparable to that seen in spontaneous prostatic hypertrophy. To investigate the mechanism of this synergism, cytosol androgen binding was measured by a density gradient technique in prostates of control and 17 beta-estradiol-treated castrate dogs. [3H]Dihydrotestosterone was bound principally to a moiety that averaged 8.6S in size. Approximately twofold enhancement of this binding by 17 beta-estradiol was demonstrable after 1 wk of treatment with 750 microgram/wk and after 3 wk with 75 microgram/wk. Under conditions in which binding in the 8S region was demonstrable with dihydrotestosterone and testosterone no binding of 3 alpha-androstanediol or progesterone was detectable. Thus, enhancement by 17 beta-estradiol of a prostate cytosol androgen-binding protein occurs under circumstances in which 17 beta-estradiol enhances androgen-mediated prostatic growth.
D000818:Animals; D002369:Castration; D003593:Cytoplasm / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D013196:Dihydrotestosterone / Q000378:metabolism*; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000494:pharmacology*; D008297:Male; D011467:Prostate / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism; D013997:Time Factors
D011956:Receptors, Cell Surface; D013196:Dihydrotestosterone; D004958:Estradiol