title: Two temperature-sensitive polA mutants: an approach to the role in vivo of  DNA polymerase I.
abstract: DNA polymerase I (Pol I) purified from a strain carrying polA12 demonstrates a defect primarily in the polymerase portion of the molecule. The mutant enzyme is altered in its structure and template specificity. Pol I isolated from strains carrying polAexl are primarily defective in 5' in equilibrium 3' exonuclease. The polAexl lesion renders strains conditonally lethal.
mesh_terms: D004254:DNA Nucleotidyltransferases / Q000378:metabolism*; D004355:Drug Stability; D004926:Escherichia coli / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D009154:Mutation; D013696:Temperature
chemical_list: D004254:DNA Nucleotidyltransferases