A plaque assay for the simian rotavirus SAII.
A sensitive, quantitative and reproducible plaque assay for the measurement of the simian rotavirus SAII is described. Plaque formation required the presence of the facilitators pancreatin or trypsin and diethylaminoethyl-dextran in the agar overlay. SAII produced plaques in three continuous primate cell lines: MA-104, CV-1 and LLC-MK2. MA-104 cells were the most sensitive.
D000818:Animals; D002460:Cell Line; D003637:DEAE-Dextran / Q000494:pharmacology; D000882:Haplorhini; D007668:Kidney; D010194:Pancreatin / Q000494:pharmacology; D012328:RNA Viruses / Q000302:isolation & purification*; D012401:Rotavirus / Q000187:drug effects / Q000302:isolation & purification*; D013552:Swine; D014357:Trypsin / Q000494:pharmacology; D010948:Viral Plaque Assay / Q000379:methods*; D014779:Virus Replication / Q000187:drug effects
D010194:Pancreatin; D003637:DEAE-Dextran; D014357:Trypsin