Chicken liver amidophosphoribosyltransferase. Ligand-induced alterations in molecular properties.
A homogeneous amidophosphoribosyltransferase (EC 2.4.2.14) preparation, which was sensitive to purine nucleotide inhibitors, was obtained from chicken liver. From the result of sodium dodecyl sulfate polyacrylamide gel electrophoresis, the subunit weight was estimated to be approximately 58 000. In Tris-HCl buffer, the predominant form of the enzyme had an S20,w of 6.5, Strokes radius of 40 A, and estimated molecular weight of 110 000. Incubation with 5-phosphoribosyl 1-pyrophosphate or Pi resulted in an increase in the S20,w to 9.1--9.5, Strokes radius 50 A, and estimated molecular weight to 200 000. Incubation of the large form with AMP led to a decrease in the molecular wight of the enzyme. It is concluded that chicken liver amidophosphoribosyltransferase is an allosteric protein whose activity is regulated by a series of conformational changes induced by a number of ligands.
D000249:Adenosine Monophosphate; D000582:Amidophosphoribosyltransferase / Q000302:isolation & purification / Q000378:metabolism; D000818:Animals; D002645:Chickens; D006157:Guanosine Monophosphate; D007700:Kinetics; D008024:Ligands; D008099:Liver / Q000201:enzymology*; D046911:Macromolecular Substances; D008274:Magnesium; D008970:Molecular Weight; D010430:Pentosyltransferases / Q000378:metabolism; D010754:Phosphoribosyl Pyrophosphate; D011485:Protein Binding; D011487:Protein Conformation
D008024:Ligands; D046911:Macromolecular Substances; D000249:Adenosine Monophosphate; D010754:Phosphoribosyl Pyrophosphate; D006157:Guanosine Monophosphate; D010430:Pentosyltransferases; D000582:Amidophosphoribosyltransferase; D008274:Magnesium