[Dextran glucosidase activity of the acid alpha-glucosidase from pig spleen].
Some properties of acid alpha-glucosidase from pig spleen are studied. Changes in the enzyme activity with respect to polymeric and oligomeric substrates were shown to take place under effects of temperature, pH, concentration of metal ions, urea treatment, trehalose inhibition. Dextran and glycogen were used as polymeric substrates, and disaccharides with different types of bonds were taken as oligomeric substrates. The data obtained confirm the hypothesis on separate regions of binding polymers and oligomers with the enzyme molecule.
D000818:Animals; D002414:Cations, Monovalent; D002846:Chromatography, Affinity; D003911:Dextrans / Q000378:metabolism / Q000494:pharmacology*; D004355:Drug Stability; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation / Q000187:drug effects; D004794:Enzyme Repression / Q000187:drug effects; D005959:Glucosidases / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008970:Molecular Weight; D009844:Oligosaccharides / Q000378:metabolism; D011188:Potassium / Q000494:pharmacology; D012964:Sodium / Q000494:pharmacology; D013154:Spleen / Q000201:enzymology*; D013552:Swine; D013696:Temperature; D014199:Trehalose / Q000494:pharmacology; D014508:Urea / Q000494:pharmacology
D002414:Cations, Monovalent; D003911:Dextrans; D009844:Oligosaccharides; D014508:Urea; D012964:Sodium; D014199:Trehalose; D005959:Glucosidases; D011188:Potassium
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