[Influence of cyclic adenosine monophosphate on the migration of primordial germ cells of the anuran Amphibians (author's transl)].
After the treatment in toto of the embryos from various species of Anura by cAMP, the number of primordial germ cells (PGC) in genital ridges is strongly reduced; the most part of the PGC are found in the endoderm. A ventral implant of agar impregnated with a solution of cAMP attracts numerous PGC in the same way as grafted chordomesoderm. The chordomesoderm itself, incubated with 3'-5' cyclic nucleotide phosphodiesterase, then grafted on to the ventral area of normal embryos attracts very few PBC compared with the non-incubated chordomesoderm. The results are analyzed and discussed along the following hypothesis: the migration of the PGC of the Anura is guided by cAMP which, diffusing from the chordomesoderm, is distributed along a concentration gradient increasing in a ventrodorsal direction. The PGC go up the gradient by a chemiotactic mechanism and reach the dorsal parts of the embryo.
D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000494:pharmacology; D000663:Amphibians / Q000196:embryology*; D000818:Animals; D001001:Anura; D002023:Bufo bufo; D002465:Cell Movement / Q000187:drug effects; D000242:Cyclic AMP / Q000494:pharmacology*; D005854:Germ Cells / Q000187:drug effects*; D011896:Rana temporaria; D014981:Xenopus
D000242:Cyclic AMP; D015105:3',5'-Cyclic-AMP Phosphodiesterases
Influence de l'adénosine monophosphate cyclique sur la migration des cellules germinales primordiales des amphibiens anoures.