Kinetic study of neutrophil and inflammatory fluid beta glucuronidase.
Beta glucuronidase obtained from rat inflammatory fluid and granulocytes was studied kinetically. Linear increases in activity occurred with time and enzyme concentration. No evidence of enzyme degradation during incubation was obtained. Supernatant and granulocyte enzyme activity was identical in relation to Km, and pH optima and resistance to high NaCl concentration. Minimal contributions to supernatant activity occur from plasma and red cells.
D000818:Animals; D005122:Exudates and Transudates / Q000201:enzymology*; D005260:Female; D005966:Glucuronidase / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D066298:In Vitro Techniques; D007249:Inflammation / Q000201:enzymology*; D007700:Kinetics; D009504:Neutrophils / Q000201:enzymology*; D010447:Peptide Hydrolases; D051381:Rats; D012965:Sodium Chloride / Q000494:pharmacology
D012965:Sodium Chloride; D005966:Glucuronidase; D010447:Peptide Hydrolases