Serum dopamine beta-hydroxylase: assay and enzyme properties.
A method for the estimation of dopamine beta-hydroxylase activity in human serum is described, based on a thin layer chromatographic separation of the substrate ([14C]tyramine) from the reaction product ([14C]octopamine). The basic properties of the human serum enzyme, investigated by this method are described.
D002849:Chromatography, Gas; D002855:Chromatography, Thin Layer; D003300:Copper / Q000494:pharmacology; D004299:Dopamine beta-Hydroxylase / Q000037:antagonists & inhibitors / Q000097:blood*; D004355:Drug Stability; D004492:Edetic Acid / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009655:Octopamine / Q000097:blood; D013696:Temperature; D014439:Tyramine / Q000097:blood
D009655:Octopamine; D003300:Copper; D004492:Edetic Acid; D004299:Dopamine beta-Hydroxylase; D014439:Tyramine