Effects of amino acids on calcium uptake by glial and neuroblastoma cells.
The uptake of [45Ca] has been studied in clonal glial and neuronal cells. It was somewhat more efficient in the neuroblastoma clone M1 compared to glial clones. In all cases [45Ca] uptake was shown to depend on the phosphate concentration in the incubation medium. It was decreased by the ionophore A 23187  at 200 microM concentration in both neuronal and glial clones. The influence of amino acids some of which are putative neurotransmitters was investigated; the interactions between [45Ca] uptake and these amino acids were related to their concentration and the type of cells used (neuronal or glial). L-aspartate and taurine for example had two opposite effects on [45Ca] uptake by the glial clone NN at two different concentrations; they could therefore play a role in the control of calcium level in the synaptic cleft.
D000596:Amino Acids / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D001224:Aspartic Acid / Q000494:pharmacology; D001932:Brain Neoplasms / Q000378:metabolism*; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002999:Clone Cells / Q000378:metabolism; D006224:Cricetinae; D009447:Neuroblastoma / Q000378:metabolism*; D009457:Neuroglia / Q000378:metabolism*; D010710:Phosphates / Q000494:pharmacology; D009435:Synaptic Transmission / Q000187:drug effects*; D013654:Taurine / Q000494:pharmacology
D000596:Amino Acids; D010710:Phosphates; D013654:Taurine; D001224:Aspartic Acid; D000001:Calcimycin; D002118:Calcium