A spin-label study of the effect of gamma radiation on erythrocyte membrane. Influence of lipid peroxidation on membrane structure.
Gamma-irradiation of bovine erythrocyte membranes (0.1-4 Mrad) resulted in a decrease in the degree of order of membrane lipids, as measured by spin-labelled fatty acid esters, at the depth of C12 but not at the depth of C5. Dose dependence of this phenomenon corresponded to dose dependence of malondialdehyde formation in the membranes. On this basis a mechanism for the effect of lipid peroxidation on the membrance structure is proposed. Membrane proteins underwent radiation-induced conformational transitions revealed by maleimide spin label which could be also connected with lipid peroxidation.
D000818:Animals; D002417:Cattle; D004578:Electron Spin Resonance Spectroscopy; D004910:Erythrocyte Membrane / Q000378:metabolism / Q000528:radiation effects*; D004912:Erythrocytes / Q000528:radiation effects*; D008563:Membrane Lipids / Q000378:metabolism* / Q000528:radiation effects; D008565:Membrane Proteins / Q000378:metabolism / Q000528:radiation effects*; D010084:Oxidation-Reduction; D011487:Protein Conformation / Q000528:radiation effects; D013113:Spin Labels
D008563:Membrane Lipids; D008565:Membrane Proteins; D013113:Spin Labels