Polymorphism of apolipoprotein E. I. Methodological aspects and diagnosis of hyperlipoproteinemia type III without ultracentrifugation.
Two methods for phenotyping apolipoprotein E are compared. One is based on preparation of VLDL by conventional ultracentrifugation, whereas the other uses heparin/Mg precipitation of VLDG. In principle, the same results were obtained by both methods. However, the group limits for the three different phenotypes Apo E-N, Apo E-ND and Apo E-D were slightly different by the two methods. Phenotype Apo E-D - the phenotype characterizing type III dyslipoproteinemia - is clearly definable by both methods. Hence the precipitation-I.E.F. method for Apo E phenotyping provides a simple tool for genetic and population genetic studies and also for the routine diagnosis of hyperlipoproteinemia type III, based on the only specific marker known for this disease.
D001053:Apolipoproteins / Q000235:genetics*; D011232:Chemical Precipitation; D003720:Densitometry; D005069:Evaluation Studies as Topic; D006493:Heparin; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood / Q000175:diagnosis* / Q000235:genetics*; D007525:Isoelectric Focusing; D008079:Lipoproteins, VLDL / Q000032:analysis / Q000302:isolation & purification; D008274:Magnesium; D008722:Methods; D010641:Phenotype; D011110:Polymorphism, Genetic; D014461:Ultracentrifugation
D001053:Apolipoproteins; D008079:Lipoproteins, VLDL; D006493:Heparin; D008274:Magnesium