A micromethod for sphingomyelinase assay using a chromogenic artificial substrate. Its use in the diagnosis of Niemann-Pick disease.
D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D002794:Choline / Q000031:analogs & derivatives*; D005260:Female; D005347:Fibroblasts / Q000201:enzymology; D006801:Humans; D008297:Male; D008722:Methods; D009542:Niemann-Pick Diseases / Q000175:diagnosis*; D009596:Nitrophenols; D010727:Phosphoric Diester Hydrolases / Q000032:analysis*; D010767:Phosphorylcholine / Q000031:analogs & derivatives*; D013108:Sphingomyelin Phosphodiesterase / Q000032:analysis*
D009596:Nitrophenols; D010767:Phosphorylcholine; C010733:2-hexadecanoylamino-4-nitrophenylphosphorylcholine; D010727:Phosphoric Diester Hydrolases; D013108:Sphingomyelin Phosphodiesterase; D002794:Choline