Detection of Epstein-Barr virus-coded antigens in lymphocytes isolated from defined patient samples.
A preliminary report on the use of specific rabbit antisera raised to Epstein-Barr virus-coded antigens (EBNA and EA) for detection of these antigens in vivo is presented. Human lymphocytes were isolated on isokinetic gradients and the C3 receptor-bearing B-lymphocyte subpopulation was isolated, providing an enriched source of EBV-infected lymphocytes. Such technology was employed to establish the status of the EBV host-cell complex in recurrent exudative tonsillitis (RET), infectious mononucleosis (IM), and Hodgkin's and non-Hodgkin's lymphoma patients. Only EBNA was detected in the lymphocytes from the tonsils of RET patients and the peripheral blood of IM patients. However, the spleen and lymph-nodes of patients with lymphomas had lymphocytes synthesizing EBNA and EA.
D000956:Antigens, Viral / Q000302:isolation & purification*; D001402:B-Lymphocytes / Q000276:immunology*; D002467:Cell Nucleus / Q000276:immunology; D003176:Complement C3; D005455:Fluorescent Antibody Technique; D004854:Herpesvirus 4, Human / Q000276:immunology*; D006689:Hodgkin Disease / Q000276:immunology; D006801:Humans; D007244:Infectious Mononucleosis / Q000276:immunology; D008223:Lymphoma / Q000276:immunology; D014069:Tonsillitis / Q000276:immunology
D000956:Antigens, Viral; D003176:Complement C3