Comparative study on hydrolases in five species of Ochromonas (chrysomonadina).
Acid phosphatase and beta-glucosidase were shown to be present in five species of Ochromonas grown in organic media (O. danica, O. malhanesis, O. munuta, O. sociabilis and Ochromonas sp. 933/4). Acid phosphatase was found to have a pH optimum at 4.0 in O. danica, and at 5.1 in the four other species. No alkaline phosphatase was found in any of the above mentioned species. Beta-glucosidase in the species studied has a pH optimum at 4.6. Low alpha-glucosidase activity was found only in O. danica. Acid phosphatase in all the five species shows an increase in activity during the logarithmic phase of growth and a decrease during the early stationary phase. Beta-glucosidase shows a similar behavior only in O. danica.
D000135:Acid Phosphatase / Q000032:analysis; D000367:Age Factors; D000469:Alkaline Phosphatase / Q000032:analysis; D003470:Culture Media; D056890:Eukaryota / Q000201:enzymology* / Q000254:growth & development; D005959:Glucosidases / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D006867:Hydrolases / Q000032:analysis*; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis; D013045:Species Specificity
D003470:Culture Media; D006867:Hydrolases; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D010744:Phosphoric Monoester Hydrolases; D005959:Glucosidases