Horse muscle acylphosphatase: a more rapid purification procedure and crystallization.
A crystalline acyl phosphatase has been obtained from horse muscle. The enzyme purified by a modified procedure which allowed to obtain a larger yield with respect to a method previously developped in this laboratory, was homogeneous by disc electrophoresis on polyacrylamide gel. Crystals of this enzymatic protein have been obtained by the addition of ammonium sulphate to around 52% saturation.
D017766:Acid Anhydride Hydrolases; D000818:Animals; D011232:Chemical Precipitation; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003460:Crystallization; D004591:Electrophoresis, Polyacrylamide Gel; D005374:Filtration; D006736:Horses; D009132:Muscles / Q000201:enzymology*; D010755:Organophosphates / Q000302:isolation & purification; D010744:Phosphoric Monoester Hydrolases / Q000302:isolation & purification*; D000097024:Acylphosphatase
D017766:Acid Anhydride Hydrolases; D010755:Organophosphates; D010744:Phosphoric Monoester Hydrolases; D000097024:Acylphosphatase