A procedure for the quantitation of relaxed closed circular DNA in the presence of superhelical DNA: an improved fluorometric assay for nicking-closing enzyme.
D000818:Animals; D001435:Bacteriophages / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D004264:DNA Topoisomerases, Type I / Q000378:metabolism*; D004270:DNA, Circular / Q000032:analysis*; D004278:DNA, Superhelical; D004279:DNA, Viral / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D000882:Haplorhini; D010727:Phosphoric Diester Hydrolases; D013539:Simian virus 40 / Q000201:enzymology; D012910:Snake Venoms; D013050:Spectrometry, Fluorescence
D004270:DNA, Circular; D004278:DNA, Superhelical; D004279:DNA, Viral; D012910:Snake Venoms; D010727:Phosphoric Diester Hydrolases; D004264:DNA Topoisomerases, Type I