Studies on guinea-pig macrophage migration inhibitory factor (MIF). I. Glycoprotein nature and net charge.
Guinea-pig macrophage migration inhibitory factor (MIF), obtained by the stimulation of sensitized lymph node cells with tuberculin PPD, was characterized as a glycoprotein by the following criteria: (a) its activity is destroyed by 0.02 M sodium periodate; (b) when MIF-containing culture fluids are subjected to precipitation by perchloric acid (final concentration 1 M), the inhibitory activity is recovered in the supernatant; and (c) MIF binds to Sepharose-linked concanavalin A and can be eluted with methyl-alpha-D-glucopyranoside. When MIF-containing culture supernatants are fractionated by isoelectrofocussing, migration inhibitory activity is recovered in a fraction with an isoelectric point of 4.4--4.6.
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D002846:Chromatography, Affinity; D003208:Concanavalin A; D006023:Glycoproteins; D006168:Guinea Pigs; D007525:Isoelectric Focusing; D008263:Macrophage Migration-Inhibitory Factors / Q000037:antagonists & inhibitors / Q000302:isolation & purification; D008297:Male; D010472:Perchlorates; D010504:Periodic Acid / Q000494:pharmacology; D012685:Sepharose
D006023:Glycoproteins; D008263:Macrophage Migration-Inhibitory Factors; D010472:Perchlorates; D010504:Periodic Acid; D003208:Concanavalin A; D012685:Sepharose