title: Separation of myocardial cytoplasmic acidic inhibitor proteins which inhibit hepatic protein biosynthesis in vitro.
abstract: Cytoplasmic inhibitor proteins of rabbit and pig myocardium have been separated and their in-vitro effects on hepatic cell-free protein biosynthesis reported. Three protein peaks could be separated from myocardial cytoplasmic pH 5 fraction by DEAE and CM cellulose column chromatography. There was a difference in the elution pattern of the acidic inhibitor proteins obtained from pig and rabbit myocardium. The myocardial acidic inhibitor proteins inhibited a liver cell-free protein biosynthesizing system, but not a myocardial cell-free system.
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002250:Carbon Radioisotopes; D002848:Chromatography, DEAE-Cellulose; D003402:Creatine Kinase / Q000378:metabolism; D003593:Cytoplasm / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000378:metabolism*; D008297:Male; D009124:Muscle Proteins / Q000302:isolation & purification*; D009206:Myocardium / Q000032:analysis* / Q000378:metabolism; D010649:Phenylalanine / Q000378:metabolism; D014176:Protein Biosynthesis; D011506:Proteins / Q000037:antagonists & inhibitors; D011817:Rabbits; D012270:Ribosomes / Q000378:metabolism; D013552:Swine
chemical_list: D000596:Amino Acids; D002250:Carbon Radioisotopes; D009124:Muscle Proteins; D011506:Proteins; D010649:Phenylalanine; D003402:Creatine Kinase