Some observations on the phosphatase cytochemistry of the submandibular gland of cat.
The localization of reaction product arising from incubation with thiamine pyrophosphate at pH 7.2 and beta-glycerophosphate or nitrophenylphosphate at pH 5 has been examined in central and demilunar acinar cells, myoepithelial cells, and cells of the striated ducts. Thiamine pyrophosphate reaction product was seen in the inner saccules of the Golgi apparatuses of the different types of cell. beta-Glycerophosphate and nitrophenylphosphate reaction products were seen in lysosomes, and in GERL-like structures in all except the cells of the striated ducts, in which the reaction products were observed in the Golgi apparatus. In central cells, the latter two reactions products were also seen in secretory granules and the cytosol.
D000818:Animals; D002415:Cats; D006651:Histocytochemistry; D008854:Microscopy, Electron; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis*; D013347:Subcellular Fractions / Q000201:enzymology / Q000648:ultrastructure; D013363:Submandibular Gland / Q000201:enzymology* / Q000648:ultrastructure; D013379:Substrate Specificity; D013835:Thiamine Pyrophosphate
D010744:Phosphoric Monoester Hydrolases; D013835:Thiamine Pyrophosphate