Effects of protease inhibitors on chemical induction of type C virus.
A role for proteolysis during chemical induction of endogenous xenotropic Type C virus from Kirsten sarcoma virus-transformed mouse cells was examined. Two distinct classes of protease inhibitors, the trypsin inhibitor, alpha-N-tosyl-L-lysine chloromethyl ketone, and two naturally occurring oligopeptide inhibitors, antipain and leupeptin, were found to inhibit induction of virus by cycloheximide and histidinol. Virus activation by 5-iododeoxyuridine was inhibited to a lesser degree. During the time cells were exposed to these compounds, there was little inhibition of [3H]uridine incorporation into total cellular RNA or polyadenylic acid cytoplasmic messenger RNA, suggesting that inhibition of proteolysis, and not RNA transcription, was responsible for blocking virus induction.
D000818:Animals; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D002999:Clone Cells; D003513:Cycloheximide / Q000037:antagonists & inhibitors; D007065:Idoxuridine / Q000037:antagonists & inhibitors; D051379:Mice; D011480:Protease Inhibitors / Q000494:pharmacology*; D012190:Retroviridae / Q000187:drug effects*; D014107:Tosyllysine Chloromethyl Ketone / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects; D014779:Virus Replication / Q000187:drug effects*
D011480:Protease Inhibitors; D014107:Tosyllysine Chloromethyl Ketone; D003513:Cycloheximide; D007065:Idoxuridine