[Semiautomatic method of measurement of serum 5'nucleotidase activity].
A semi-automated method for determining the 5'-nucleotidase activity in human serum for use with the Technicon Autoanalyzer I is described. Based upon the manual method of Persijn and Van Der Slik, adenosine formed by hydrolysis of 5'-AMP is deamined enzymatically and ammonia determined with the Berthelot reaction. The non specific alkaline phosphatases are inhibited by phenylphosphate. Concentrations of 5'-AMP and phenylphosphate are discussed. Nucleotidase activity is determined without dilution up to a value of 210 UI/liter. The precision is better than other manual or semi-automated methods in current use.
D000243:Adenosine Deaminase; D000249:Adenosine Monophosphate; D000328:Adult; D000469:Alkaline Phosphatase; D000641:Ammonia / Q000097:blood; D001322:Autoanalysis / Q000379:methods*; D001555:Benzene Derivatives; D003124:Colorimetry; D005260:Female; D006505:Hepatitis / Q000201:enzymology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008274:Magnesium; D008297:Male; D008875:Middle Aged; D009708:Nucleotidases / Q000097:blood*; D010001:Osteitis Deformans / Q000201:enzymology; D010710:Phosphates; D012279:Rickets / Q000201:enzymology; D013696:Temperature
D001555:Benzene Derivatives; D010710:Phosphates; D000249:Adenosine Monophosphate; D000641:Ammonia; D009708:Nucleotidases; D000469:Alkaline Phosphatase; D000243:Adenosine Deaminase; D008274:Magnesium
Méthode semi-automatique de mesure d'activité de la 5'-nucléotidase sérique