title: Preparation and assay of purified Escherichia coli polysomes devoid of free ribosomal subunits and endogenous GTPase activities.
mesh_terms: D002458:Cell Fractionation / Q000379:methods; D004926:Escherichia coli / Q000201:enzymology / Q000648:ultrastructure*; D020963:GTP Phosphohydrolase-Linked Elongation Factors / Q000032:analysis*; D010441:Peptide Chain Elongation, Translational; D010445:Peptide Elongation Factors / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis*; D011132:Polyribosomes / Q000201:enzymology / Q000648:ultrastructure*; D011691:Puromycin / Q000378:metabolism; D012343:RNA, Transfer / Q000378:metabolism
chemical_list: D010445:Peptide Elongation Factors; D011691:Puromycin; D012343:RNA, Transfer; D010744:Phosphoric Monoester Hydrolases; D020963:GTP Phosphohydrolase-Linked Elongation Factors