[Effect of sodium n-dipropylacetate (sodium valproate) on GABA level of neuronal and glial cells in culture].
The effect of sodium n dipropylacetate (nDPA), a competitive GABA-T inhibitor with respect to GABA, has been investigated on glial and neuronal cellular GABA level. After 1 to 4 days incubation with nDPA in the culture medium, a decrease of GABA level in M5 neuroblastoma clonal cell lines and no modification of GABA level in C6 astrocytoma cells has been observed. The combined addition of nDPA 4 micrometer with dibutyryl cyclic AMP (1 mM) to the culture medium induces the same decrease in GABA level in C6 astrocytoma cells as the addition of DB-c-AMP alone. After shorter incubation time with nDPA (5-150 min), we observed a decreased GABA level in C6 astrocytoma glial cells.
D000818:Animals; D001254:Astrocytoma; D003994:Bucladesine / Q000494:pharmacology; D002460:Cell Line; D007700:Kinetics; D009447:Neuroblastoma; D051381:Rats; D014635:Valproic Acid / Q000494:pharmacology*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D005680:gamma-Aminobutyric Acid; D014635:Valproic Acid; D003994:Bucladesine
Effet du n-dipropylacétate de sodium (valproate de sodium) sur la teneur en GABA de cellules neuronales et gliales en culture.