[A kinetic method for the determination of the activity of "aerobic transhydrogenases" (author's transl)].
The chromogen ABTS is the di-ammonium salt of 2,2'-azino-di[3-ethyl-benzthiazolin-sulfonic acid (6)] routinely used in the "glucose-oxidase assay" with the peroxidase (GOD-Perid method, Boehringer). 1. The specific property of ABTS to give a stable radical cation by oxidation with hydrogen peroxide in the presence of peroxidase was used to design a kinetic method, for enzyme-activity determinations. 2. The assay is suitable for the specific oxido-reductase using oxygen as acceptor, known also as "aerobic transhydrogenases" which are H2O2 formers (EC 1.-.3.-). 3. L-Amino acid: oxygen oxidoreductase (deaminating) (EC 1.4.3.2), was used throughout, being a representative model for such determinations.
D000332:Aerobiosis; D000594:Amino Acid Oxidoreductases / Q000032:analysis; D006861:Hydrogen Peroxide; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010088:Oxidoreductases / Q000032:analysis*; D010544:Peroxidases; D013844:Thiazoles
D013844:Thiazoles; D006861:Hydrogen Peroxide; D010088:Oxidoreductases; D010544:Peroxidases; D000594:Amino Acid Oxidoreductases
Eine kinetische Methode zur Bestimmung von "Aeroben Transhydrogenasen"