[Determination of bilirubin in the amniotic fluid by means of the chloroform-extraction method].
A reproducible method for the determination of bilirubin in amniotic fluid by measuring the extinction of the the chloroform extract is described. The measurements can be carried out by means of a simple spectrophotometer. In case of extraction of amniotic fluid containing blood informative results are obtained as well. Since the extinction values are equal to the deltaE-values it is possible to determine the degree of morbus haemolyticus fetalis using the schema of Liley.
D000653:Amniotic Fluid / Q000032:analysis*; D001663:Bilirubin / Q000032:analysis*; D002498:Centrifugation; D002725:Chloroform; D004899:Erythroblastosis, Fetal / Q000175:diagnosis; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D011247:Pregnancy
D002725:Chloroform; D001663:Bilirubin
Bestimmung von Bilirubin im Fruchtwasser mit der Chloroformextraktionsmethode