title: Studies of membrane fusion. IV. Fusion of HeLa cells with Sendai virus.
abstract: The Sendai virus-induced fusion of HeLa cells has been studied by freeze-fracture electron microscopy. Freeze-fracture observations confirm previous scanning electron-microscope studies (1977) and show that at 4 degrees C virus particles bind to the cell surface and that cell agglutination results from the crosslinking by virus particles of microvilli on adjacent cells. Incubation at 37 degrees C initiates a change in viral envelope structure and fusion of 'altered' virus particles with the cell plasma membrane. Fusion of a virus particle with two crosslinked cells is probably the membrane fusion event which initiates cell-cell fusion; fusion is completed as a result of virally induced cell swelling. Lateral diffusion of viral envelope components following virus-cell fusion and, in some instances, an aggregation of plasma membrane intramembrane particles occurs in swollen cells. These observations show that the mechanisms of viral envelope-cell and probably cell-cell fusion are the same as have been reported for erythrocytes. Although endocytosis of intact virus particles does occur, the specialized cell-mediated mechanism for fusion of the viral envelope with the cell plasma membrane suggests that this, and not viropexis, is the mechanism of Sendai virus infection.
mesh_terms: D000371:Agglutination; D002449:Cell Aggregation; D002459:Cell Fusion; D002462:Cell Membrane / Q000648:ultrastructure; D005614:Freeze Fracturing; D006367:HeLa Cells / Q000648:ultrastructure*; D006801:Humans; D010222:Parainfluenza Virus 1, Human / Q000502:physiology* / Q000648:ultrastructure