Arrangement of glucose residues in the lipid-linked oligosaccharide precursor of asparaginyl oligosaccharides.
The lipid-linked oligosaccharide synthesized in vitro, in the presence of 1.0 microM UDP-[3H]Glc, GDP-[14C]Man, and UDP-GlcNAc has been isolated and the structure of the oligosaccharide has been analyzed. The oligosaccharide contains 2 N-acetylglucosamine, 9 mannose, and 3 glucose residues. The N-acetylglucosamine residues are located at the reducing terminus. The 3 glucose residues are arranged in a linear order at one of the nonreducing termini in the sequence Glc 1,2--Glc 1,3--Glc--(Man)9 (GlcNAc)2. The structural analysis was made possible largely by the availability of glucosidase preparations of fungal anad microsomal origin which remove glucose residues from the oligosaccharide without releasing mannose residues.
D001216:Asparagine; D002460:Cell Line; D005947:Glucose / Q000032:analysis*; D008361:Mannosidases; D008745:Methylation; D009844:Oligosaccharides; D010084:Oxidation-Reduction; D010504:Periodic Acid; D012845:Sindbis Virus / Q000032:analysis
D009844:Oligosaccharides; D010504:Periodic Acid; D001216:Asparagine; D008361:Mannosidases; D005947:Glucose