The effect of mucin on the bioavailability of tetracycline from the gastrointestinal tract; in vivo, in vitro correlations.
The bioavailability of tetracycline in the presence and absence of a porcine gastric mucin dispersion (1% W/V) has been studied by in vivo perfusion of the rat small intestine, the rat everted gut, a diffusion cell technique and the Sartorius Absorption Simulator Apparatus. In the presence of the mucin preparation an approximate 50% reduction in the numerical values of each of the parameters used to measure the tetracycline movement across the membrane was found. The physiological significance of each technique and the methods themselves are discussed. Interaction of tetracycline with the porcine gastric mucin preparation is considered in relation to the molecular structure of mucus. The results suggest the drug is bound in some way, as yet undefined, to the mucin macromolecule.
D000818:Animals; D001682:Biological Availability; D004058:Diffusion; D066298:In Vitro Techniques; D007408:Intestinal Absorption / Q000187:drug effects*; D007413:Intestinal Mucosa / Q000378:metabolism; D008297:Male; D009077:Mucins / Q000494:pharmacology*; D010477:Perfusion; D010539:Permeability; D051381:Rats; D013752:Tetracycline / Q000378:metabolism*
D009077:Mucins; D013752:Tetracycline