The molecular weight of bovine lactoferrin.
The molecular weight of bovine lactoferrin in a neutral solvent (e.g. 0.2 M NaC1, pH 7.8 20 degrees C) has been determined using high-speed sedimentation equilibrium at various concentrations and speeds. Under these conditions the protein behaved as a single thermodynamic component with a weight-average molecular weight [MW]C=0 = 93000 plus or minus 2000 and a second virial coefficient B = 4.3-10(-5) mol-ml(-1)-g(-2). This value of the molecular weight is in good agreement with values previously reported by Groves (J. Am. Chem. Soc. 82, 3345-3350, 1960) and Szuchet and Johnson (Eur. Polym. J. 2, 115-128, 1966); it is, however, in disagreement with the more recent value given by Castellino et al. (J. Biol. Chem. 245, 4269-4275, 1970). The possible reasons for this discrepancy are discussed.
D000818:Animals; D002417:Cattle; D003201:Computers; D005069:Evaluation Studies as Topic; D006863:Hydrogen-Ion Concentration; D007781:Lactoferrin; D007782:Lactoglobulins; D046911:Macromolecular Substances; D008433:Mathematics; D008722:Methods; D008970:Molecular Weight; D013816:Thermodynamics; D014461:Ultracentrifugation
D007782:Lactoglobulins; D046911:Macromolecular Substances; D007781:Lactoferrin