Transcription of Epstein-Barr virus genomes in human lymphoblastoid cells and in somatic-cell hybrids of Burkitt's lymphoma.
Expression of latent Epstein-Barr virus genomes in lymphoblastoid cells and somatic-cell hybrid of Burkitt's lymphoblastoid cells has been studied. IUDR treatment induced the formation of early antigen (EA), virus capsid antigen (VCA) and virus DNA replication in D98/Raji and D98/HR-1 cells whereas only EA was induced in Raji cells. HR-1 clone No. 9 did not respond to IUDR treatment. The pattern of transcription of virus genomes in these cell lines without IUDR treatment was uniform with 20--25% of virus DNA transcribed. IUDR treatment enhanced the transcription of virus DNA to 50% in D98/Raji, D98/HR-1 and Raji cells but no enhancement of virus genome transcription was observed in HR-1 clone No. 9 The amount of virus RNA in the cells calculated from DNA-RNA hybridization kinetics was found to be proportional to the number of virus genomes per cell indicating that every copy of virus DNA in these cells is actively transcribed.
D002051:Burkitt Lymphoma / Q000209:etiology*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D005814:Genes, Viral; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D006822:Hybrid Cells / Q000378:metabolism; D007065:Idoxuridine / Q000494:pharmacology; D008214:Lymphocytes / Q000378:metabolism*; D009374:Neoplasms, Experimental / Q000209:etiology; D012367:RNA, Viral / Q000096:biosynthesis; D014158:Transcription, Genetic / Q000187:drug effects
D012367:RNA, Viral; D007065:Idoxuridine