title: Changes in lipid ordering and state of aggregation in lymphocyte plasma membranes after exposure to mitogens.
abstract: An electron spin probe study was made of the effect of a number of mitogenic agents on the ordering and state of aggregation of the plasma membrane lipids of lymphocytes. These agents, which included phytohemagglutinin, Concanavalin A, the calcium ionophore A23187 and periodate, caused a 20% decrease in lipid ordering in the region of the bilayer probed by 5-nitroxide stearic acid. The corresponding methyl ester probe showed marked probe-probe interaction under the same conditions indicating an aggregation of lipids in the area probed by this label. Studies with mixed lipid vesicles and ganglioside-free cells indicate that these areas are rich in glycolipids capable of hydrogen bonding to the ester probe. The decrease in ordering and the increase in aggregation of the membrane lipids were correlated with the patching and capping of the ligand-receptor complexes. Furthermore, the disappearance of fluorescent ligand from the surface of treated cells corresponded with the return of the spectral parameters of the probes to control cell values. It was concluded that glycolipids might play an important role in ligand-induced cell surface changes either as bearers of receptor groups, as in the case of some gangliosides, or in association by hydrogen-bonding with receptor proteins.
mesh_terms: D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D003208:Concanavalin A / Q000494:pharmacology; D006224:Cricetinae; D006801:Humans; D037102:Lectins / Q000494:pharmacology; D008214:Lymphocytes / Q000378:metabolism*; D008563:Membrane Lipids / Q000378:metabolism*; D051379:Mice; D008808:Mice, Inbred CBA / Q000378:metabolism; D008934:Mitogens / Q000494:pharmacology*; D010504:Periodic Acid / Q000494:pharmacology; D013113:Spin Labels
chemical_list: D037102:Lectins; D008563:Membrane Lipids; D008934:Mitogens; D013113:Spin Labels; D010504:Periodic Acid; D003208:Concanavalin A; D000001:Calcimycin