Early effects of aminonucleoside on enzymes in the Golgi apparatus of rat liver.
Rats were injected with a single intravenous dose of aminonucleoside (AMN) and sacrificed 1-48 h later. The activity of several enzymes was assayed in the Golgi apparatus isolated from the liver. Galactosyltransferase activity showed little changes after the AMN, but both acid (EC 3.1.3.2) and alkaline phosphatase (EC 3.1.3.1) activities increased within the first hour and reached control levels only 5-24 h later. Thiamine pyrophosphatase and arylsulfatase A (EC 3.1.6.1) activities also increased and stayed at higher levels for the duration of the experiment. Arylsulfatase B (EC 3.1.6.1) activity decreased shortly after the AMN but later increased to above control levels. These findings support earlier results in which liver ultrastructural and biochemical changes were observed early before renal lesions and proteinuria.
D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002553:Cerebroside-Sulfatase / Q000378:metabolism; D002803:Chondro-4-Sulfatase / Q000378:metabolism; D005700:Galactosyltransferases / Q000378:metabolism; D006056:Golgi Apparatus / Q000187:drug effects / Q000201:enzymology*; D066298:In Vitro Techniques; D008099:Liver / Q000201:enzymology / Q000648:ultrastructure*; D008297:Male; D011691:Puromycin / Q000031:analogs & derivatives*; D011692:Puromycin Aminonucleoside / Q000494:pharmacology*; D051381:Rats; D013834:Thiamine Pyrophosphatase / Q000378:metabolism; D013997:Time Factors
D011691:Puromycin; D011692:Puromycin Aminonucleoside; D005700:Galactosyltransferases; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D002553:Cerebroside-Sulfatase; D002803:Chondro-4-Sulfatase; D013834:Thiamine Pyrophosphatase