Characterization of the mycoplasma membrane proteins. V. Release and localization of membrane-bound enzymes in Acholeplasma laidlawii.
The peripheral membrane protein fraction released by washing Acholeplasma laidlawii membranes with low-ionic strength buffers contained about 50% of the total membrane-bound ribonuclease and deoxyribonuclease activities. The ATPase, NADH oxidase and p-nitrophenylphosphatase activities remained bound to the membrane even when EDTA was added to the wash fluids, and thus appear to belong to the integral membrane protein group. Serving as a marker for peripheral membrane proteins, the membrane-bound ribonuclease activity was solubilized by bile salts much more effectively than the integral membrane-bound enzymes. On the other hand, the solubilized ribonuclease showed a much lower capacity to reaggregate with other solubilized membrane components to membranous structures. Yet, most of the ribonuclease molecules which were bound to the reaggregated membranes could not be released by low-ionic strength buffer. The reaggregated membranes differed from the native membranes in the absence of particles on their fracture faces obtained by freeze cleaving, and by their much higher labeling by the [125-I]lactoperoxidase iodination system. These results suggest that most of the proteins are exposed on the reaggregated membrane surfaces, with very little, if any, protein embedded in its lipid bilayer core. Enzyme disposition in the A. laidlawii membrane was studied by comparing the activity of isolated membranes with that of membranes of intact cells after treatment with pronase or with an antiserum to membranes. The data indicate the asymmetrical disposition of these activities, the ATPase and NADH oxidase being localized on the inner membrane surface, while the nucleases are exposed on the external membrane surface.
D000128:Acholeplasma laidlawii / Q000032:analysis / Q000201:enzymology*; D000251:Adenosine Triphosphatases / Q000032:analysis; D001426:Bacterial Proteins / Q000032:analysis; D002462:Cell Membrane / Q000032:analysis / Q000201:enzymology* / Q000648:ultrastructure; D003851:Deoxyribonucleases / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D008055:Lipids / Q000032:analysis; D008854:Microscopy, Electron; D009247:NADH, NADPH Oxidoreductases / Q000032:analysis; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis; D012260:Ribonucleases / Q000032:analysis
D001426:Bacterial Proteins; D008055:Lipids; D009247:NADH, NADPH Oxidoreductases; D003851:Deoxyribonucleases; D012260:Ribonucleases; D010744:Phosphoric Monoester Hydrolases; D000251:Adenosine Triphosphatases