Antipain lethality to Escherichia coli: dependence upon cyclic adenosine 3',5'-monophosphate and its receptor protein.
Antipain kills Escherichia coli K-12 cells in an exponential manner beginning 1 h after its addition. Mutant strains, delta cya and crp, which are unable to synthesize cyclic adenosine 3',5'-monophosphate (cAMP) and the cAMP receptor protein, respectively, are not affected. Addition of cAMP (5 mM) to antipain-treated mutant strains causes killing of delta cya cells, but not crp cells. Thus the lethal effect of antipain is dependent upon cAMP and its receptor protein.
D000242:Cyclic AMP / Q000502:physiology*; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development; D011480:Protease Inhibitors / Q000494:pharmacology*; D011953:Receptors, Cyclic AMP / Q000502:physiology*
D011480:Protease Inhibitors; D011953:Receptors, Cyclic AMP; D000242:Cyclic AMP