Inhibition of murine sarcoma virus induced transformation in an adenovirus -- NRK system.
Cell transformation induced by murine sarcoma virus (MSV-M) is significantly inhibited (80--90%) in a clonal line of normal rat kidney (NRK) cells when they are infected with rat cell passaged adenovirus 12 (R-Ad12). No inhibition is seen when R-Ad12 is added simultaneously with, or 1 1/2 or 24 hr after, MSV-M infection, suggesting that inhibition occurs most probably intracellularly. There is also a direct correlation between the extent of focus formation and the concentration of R-Ad12 used. Concentrations of R-Ad12 used to inhibit cell transformation do not affect cell growth. Significant inhibition in foci (90--100%) is also evident in the first two subcultures of R-Ad12 infected NRK cells. The mechanism of the inhibition is not yet known.
D000260:Adenoviruses, Human / Q000254:growth & development* / Q000276:immunology; D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D002999:Clone Cells; D003588:Cytopathogenic Effect, Viral; D003593:Cytoplasm / Q000276:immunology; D007668:Kidney; D051381:Rats; D009053:Sarcoma Viruses, Murine / Q000254:growth & development*; D014779:Virus Replication
D000956:Antigens, Viral