Demonstration of an ecdysteroid receptor in a cultured cell line of Drosophila melanogaster.
Incubation of the high-speed supernatant from the Kc cell line of Drosophila melanogaster with [3H]ponasterone A results in significant binding of the ligand as determined by gel filtration and dextran-coated charcoal binding assays. In vivo exposure of the Kc line to [3H]ponasterone A for 30 min results in a marked binding of the ligand by a KCl-soluble nuclear extract. With both the cytosol and nuclear preparations the binding has specificity and a low dissociation constant (3 X 10(-9) M). In addition the labeling of the nuclear preparation exhibits a saturation at approximately 7 X 10(-10) M which probably reflects the molar concentration of cytoplasmic receptors.
D001667:Binding, Competitive; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004331:Drosophila melanogaster / Q000378:metabolism*; D004440:Ecdysone / Q000378:metabolism; D004441:Ecdysterone / Q000378:metabolism; D007301:Insect Hormones / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*
D007301:Insect Hormones; D011956:Receptors, Cell Surface; D004440:Ecdysone; D004441:Ecdysterone