Metabolism of low density lipoproteins in human fibroblast culture.
The purpose of the study was to investigate the changes which low density lipoproteins (LDL) underwent during incubation with human culture fibroblasts. The results showed a predominant deposition of lipids, a low level of LDL-protein breakdown products in the cells, the altered structure of lipoproteins, absorbed on the cell surface and of lipoproteins in the incubation medium. The authors suppose intensive metabolic transformations to take place on the cell membrane. The partial lipoprotein degradation seems to realize favourable conditions for the subsequent entry of lipids into the cells and for a pinocytosis of lipoprotein particles transformed on the cell surface. The intensity of these membrane metabolic transformations is influenced by the antigenic properties of LDL, the presence of other proteins in the medium, and by the maturity and the metabolic activity of the cultured cells.
D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004622:Embryo, Mammalian; D005260:Female; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D008077:Lipoproteins, LDL / Q000378:metabolism*; D008168:Lung; D010873:Pinocytosis; D011247:Pregnancy
D003470:Culture Media; D008077:Lipoproteins, LDL