Simple process for the reduction in the nucleic acid content in yeast.
A simple one-step process for the nucleic acid reduction in Rhodotorula glutinis is described. The process consists of submitting the yeast cells to a heat treatment in an acidic (pH 2) spent medium. The optimal temperature for pH 2 medium is 90 C and the final nucleic acid content in treated yeasts was 1.2%. Heat treatment at acidic pH is preferred to that at alkaline pH because it offers a better protection for amino acids and crude protein, while being more efficient in lowering the nucleic acid level. The new process is economic and rapid and could be easily used for industrial application.
D000596:Amino Acids / Q000378:metabolism; D002852:Chromatography, Ion Exchange; D004044:Dietary Proteins; D005656:Fungal Proteins / Q000378:metabolism; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D003904:Mitosporic Fungi / Q000378:metabolism*; D009696:Nucleic Acids / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors
D000596:Amino Acids; D004044:Dietary Proteins; D005656:Fungal Proteins; D009696:Nucleic Acids