Requirement of galactolipids for photosystem I activity in lyophilized spinach chloroplasts.
1. The effect of monogalactosyl diacylglycerol and digalactosyl diacylglycerol on reconstitution of Photosystem I activity in heptane-extracted and galactolipase-treated spinach chloroplasts was investigated. 2. Both galactolipids, in a molar ratio with chlorophyll of 2.5, partially restored Photosystem I activity in heptane-extracted chloroplasts. An addition of saturating amounts of plastocyanin caused complete reactivation of Photosystem I. 3. Similarly, with galactolipase-treated chloroplasts, both galactolipids partially restored Phostosystem I activity and additional amounts of plastocyanin were required for complete reactivation. 4. The action of galactolipids on partial reconstitution of Photosystem I supports the suggestion of their structural role in the restoration of thylakoid membranes.
D002736:Chloroplasts / Q000187:drug effects / Q000378:metabolism*; D004579:Electron Transport; D005612:Freeze Drying; D005690:Galactose / Q000494:pharmacology; D006017:Glycolipids / Q000494:pharmacology*; D007700:Kinetics; D008049:Lipase / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010785:Photophosphorylation / Q000187:drug effects*; D010944:Plants; D010970:Plastocyanin / Q000494:pharmacology
D006017:Glycolipids; D009249:NADP; D010970:Plastocyanin; D008049:Lipase; D005690:Galactose