title: [Effect of Vibrio cholerae filtrate on the adenyl cyclase system in vitro].
abstract: An in vitro model using homogenates of the rat intestine and liver for studying the V. Cholerae culture filtrate effect on the adenylate cyclase system is proposed. Optimal conditions for the adenylate cyclase functioning have been investigated for this model. It was shown that V. Cholerae filtrate induces a stable activation of adenylate cyclase and does not change the activity of cyclic AMP phosphodiesterase. It was also established that the activity of phosphodiesterase in the intestinal homogenate is about 2.5 fold higher than that in the liver homogenate. The model may be considered promising for investigation of the mechanism of cholerae toxin action.
mesh_terms: D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000096:biosynthesis*; D000262:Adenylyl Cyclases / Q000096:biosynthesis*; D000818:Animals; D002772:Cholera Toxin / Q000494:pharmacology*; D000242:Cyclic AMP / Q000096:biosynthesis; D004789:Enzyme Activation; D066298:In Vitro Techniques; D007421:Intestine, Small / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D008297:Male; D051381:Rats
chemical_list: D002772:Cholera Toxin; D000242:Cyclic AMP; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D000262:Adenylyl Cyclases
vernacular_title: Vliianie kholernogo fil'tra na adenilattsiklaznuiu sistemu in vitro.