Effect of biotin on ribonucleic acid synthesis.
A single injection of biotin to biotin-deficient rats produces a two-fold increase in the incorporation, both in vivo and in vitro of precursors into nucleic acids as early as 2 h after the biotin treatment. The specific activity of the precursor pool is not affected by biotin. Analysis of the polysome profile at various times following biotin treatment and a kinetic study of the effect of excess poly(U) on the incorporation of phenylalanine by cell-free amino acid incorporation experiments indicate a marked decrease in messenger-free ribosomes in rat liver after biotin administration.
D000818:Animals; D001710:Biotin / Q000172:deficiency / Q000494:pharmacology; D002467:Cell Nucleus / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008297:Male; D009963:Orotic Acid / Q000378:metabolism; D011132:Polyribosomes; D012313:RNA / Q000096:biosynthesis*; D051381:Rats; D014500:Uracil Nucleotides / Q000378:metabolism; D014804:Vitamin B Deficiency / Q000378:metabolism
D014500:Uracil Nucleotides; D009963:Orotic Acid; D012313:RNA; D001710:Biotin