Specific effect of zinc ions on DNA polymerase activity of avian myeloblastosis virus.
The effect of selected cations on DNA synthesis by DNA-polymerase of avian myeloblastosis virus (AMV) was studied. Zinc ions at low concentration (0.2mM) in the assay system enhanced the activity about 2 x fold and at higher concentration (2.0 mM) inhibited the activity completely. In contrast, addition of lithium and potassium salts produced inhibitory effects in this ionic concentration range. Replacement of K+ ion had an inhibitory effect on the activity.
D001354:Avian Leukosis Virus / Q000201:enzymology*; D009189:Avian Myeloblastosis Virus / Q000201:enzymology*; D004247:DNA / Q000096:biosynthesis; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D004789:Enzyme Activation; D008094:Lithium / Q000494:pharmacology; D019384:Nucleic Acid Synthesis Inhibitors; D011188:Potassium / Q000494:pharmacology; D015032:Zinc / Q000008:administration & dosage / Q000494:pharmacology*
D019384:Nucleic Acid Synthesis Inhibitors; D004247:DNA; D008094:Lithium; D004259:DNA-Directed DNA Polymerase; D015032:Zinc; D011188:Potassium