The location of SV40 specific sequences in the heterogeneous nuclear RNA of transformed mouse cells.
Rapidly labelled nuclear RNA from an SV40 transformed mouse 3T3 line was isolated, and different molecular size classes pooled. The fractions were treated with Ehrlich ascites exonuclease III for various lengths of time, and the digestion of the 3' ends of the RNA measured by the appearance of TCA-soluble material. The resulting partially degraded RNA populations were then hybridised to SV40 DNA. The data suggest that a high proportion of the viral specific sequences are located near the 3' ends of heterogeneous nuclear RNA.
D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral; D002499:Centrifugation, Density Gradient; D004279:DNA, Viral / Q000378:metabolism; D005092:Exonucleases / Q000378:metabolism; D007668:Kidney; D051379:Mice; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000302:isolation & purification* / Q000378:metabolism; D013539:Simian virus 40 / Q000235:genetics*
D004279:DNA, Viral; D012367:RNA, Viral; D005092:Exonucleases