On the isolation of human pituitary hormones.
Six human pituitary hormones can be obtained from a single batch of human pituitary glands by the procedure described. Starting from an acetone powder of the glands, the hormones are segregated into three fractions; one containing GH and PRL, a second containing the glycoprotein hormones, and the third fraction containing smaller peptides including ACTH and MSH. Clinical grade GH is extracted from the residue at alkaline pH, followed by a series of ammonium sulfate and pH fractionations. Further chromatography on G-100 yields a highly purified GH preparation. FSH is purified by chromatography on carboxymethyl cellulose at pH 5.4 followed by gel filtration on G-100 and fractionation on sulfopropyl Sephadex C-50 at pH 5.4. LH and TSH are separated by DEAE-cellulose chromatography at pH 9.5. The GH and glycoprotein hormones are recovered in a highly purified form and good yields. The GH is obtained in an active form which is easily soluble and well suited for clinical use. A large part of the PRL is also saved during the extraction procedure.
D000324:Adrenocorticotropic Hormone / Q000302:isolation & purification; D002845:Chromatography; D005640:Follicle Stimulating Hormone / Q000302:isolation & purification; D013006:Growth Hormone / Q000302:isolation & purification; D006801:Humans; D007986:Luteinizing Hormone / Q000302:isolation & purification; D009074:Melanocyte-Stimulating Hormones / Q000302:isolation & purification; D008722:Methods; D010902:Pituitary Gland / Q000032:analysis*; D010908:Pituitary Hormones, Anterior / Q000302:isolation & purification*; D011388:Prolactin / Q000302:isolation & purification; D013972:Thyrotropin / Q000302:isolation & purification
D010908:Pituitary Hormones, Anterior; D000324:Adrenocorticotropic Hormone; D011388:Prolactin; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D013972:Thyrotropin; D013006:Growth Hormone; D009074:Melanocyte-Stimulating Hormones