title: Characterization of avian reoviruses isolated from the synovia and breast blister.
abstract: Reoviruses Texas, S 1133, UMI 203, and WVU 2937 induced swelling of the foot pad and inflammatory changes in the synovial membranes when inoculated in the foot pad of 2-week-old gnotobiotic chicks. From differences in virus neutralization as measured with geometric mean (R) value, all four viruses are subtypes of a single serotype. The cell-associated and cell-released virus growth curves were similar, with a lag phase of about 15 hours and a log phase of 15 to 21 hours. Viral RNA synthesis reached a peak in 5 hours and was active at 14 hours but not at 18 hours. In 90 minutes at 60 C the titer of each virus had decreased about 4 logs.
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D001170:Arthritis, Infectious / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D002645:Chickens / Q000382:microbiology*; D011201:Poultry Diseases / Q000276:immunology / Q000382:microbiology*; D012367:RNA, Viral / Q000096:biosynthesis; D012087:Reoviridae / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D012088:Reoviridae Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D012867:Skin / Q000382:microbiology*; D013583:Synovial Membrane / Q000382:microbiology*
chemical_list: D000914:Antibodies, Viral; D012367:RNA, Viral