Simple and rapid method for the diagnosis of chronic granulomatous disease, measuring hydrogen peroxide and superoxide anions released from leukocytes in whole blood.
A simple quantitative method to measure release of hydrogen peroxide and superoxide anions from leukocytes using less than 10 microliter blood is described. Whole blood instead of a leukocyte suspension was used. The release of hydrogen peroxide was measured fluorometrically by adding a blood sample to an assay mixture containing homovanillic acid, peroxidase and azide. The release of superoxide anions was measured spectrophotometrically with a dual wavelength spectrophotometer by adding a blood sample to an assay mixture containing cytochrome C. The normal values of the releasing activities are given.
D003208:Concanavalin A; D003572:Cytochalasins; D006105:Granulomatous Disease, Chronic / Q000097:blood / Q000175:diagnosis*; D006801:Humans; D006861:Hydrogen Peroxide / Q000097:blood*; D007700:Kinetics; D007962:Leukocytes / Q000378:metabolism*; D010100:Oxygen / Q000097:blood*; D013053:Spectrophotometry; D013481:Superoxides / Q000097:blood*
D003572:Cytochalasins; D003208:Concanavalin A; D013481:Superoxides; D006861:Hydrogen Peroxide; D010100:Oxygen