Prolactin receptors: a comparison between chloramine-T and lactoperoxidase iodination.
The binding properties of chloramine-T iodinated oPRL (using a modified iodination procedure) to prolactin receptors of female rat liver membrane particles were compared with those of lactoperoxidase iodinated oPRL. The results indicated that both iodination methods provided 125I-oPRLs which were suitable for receptor binding studies. Our results suggested that chloramine-T 125I-oPRL was even better than lactoperoxidase 125I-oPRL in terms of lower nonspecific binding. The chloramine-T iodinated oPRL was used to study the prolactin receptors in rat ovaries and DMBA induced rat mammary tumors. The results showed that the amount of prolactin receptors in rat ovaries was related to the plasma level of prolactin as it had been reported in liver by other investigators. The study of prolactin receptors in rat mammary tumors indicated that the prolactin receptor content of hormone dependent mammary tumors was much higher than that of hormone independent mammary tumors.
D000818:Animals; D001667:Binding, Competitive; D002700:Chloramines / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D007457:Iodine Radioisotopes; D007553:Isotope Labeling / Q000379:methods; D007784:Lactoperoxidase / Q000494:pharmacology*; D008099:Liver / Q000032:analysis; D008325:Mammary Neoplasms, Experimental / Q000032:analysis; D010053:Ovary / Q000032:analysis; D010544:Peroxidases / Q000494:pharmacology*; D011388:Prolactin / Q000097:blood / Q000502:physiology*; D051381:Rats; D011956:Receptors, Cell Surface / Q000032:analysis*
D002700:Chloramines; D007457:Iodine Radioisotopes; D011956:Receptors, Cell Surface; D011388:Prolactin; D007784:Lactoperoxidase; D010544:Peroxidases