Effect of phenobarbital on cerebral energy state and metabolism. Enzymatic activities.
The changes induced by phenobarbital in cerebral enzymatic activities of the Krebs' cycle (citrate synthase, malate dehydrogenase) and electron transfer chain (total NADH-cytochrome c reductase and cytochrome oxidase) were studied. In addition, the activity of lactate dehydrogenase of acetylcholine esterase and of glutamate dehydrogenase was also studied. These enzymatic activities were evaluated in the homogenate in toto and in a crude mitochondrial fraction from rat brain. The modifications in some of these activities indicate that several new metabolic situations occur in brain tissue after phenobarbital treatment.
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000201:enzymology*; D003576:Electron Transport Complex IV / Q000378:metabolism; D004734:Energy Metabolism / Q000187:drug effects*; D005260:Female; D008291:Malate Dehydrogenase / Q000378:metabolism; D009245:NADH Dehydrogenase / Q000378:metabolism; D010634:Phenobarbital / Q000494:pharmacology*; D051381:Rats
D008291:Malate Dehydrogenase; D009245:NADH Dehydrogenase; D003576:Electron Transport Complex IV; D010634:Phenobarbital