L-asparaginase activity of Mycobacterium phlei under various growth conditions.
Seven Mycobacterium strains were grown statically on salts-glycerol-asparagine (Sauton) or on salts-glucose-glutamate (Sym) media. At desired time of incubation, the bacteria were washed with water, disintegrated with powdered corundum and in resulting cell-free extracts L-asparaginase activity was determined by the Conway method. The majority of experiments were performed on M. phlei which exhibited considerable rise in L-asparaginase activity with increasing age of the culture. This change did not occur on Sym medium because of Zn2+, which proved to abolish the effect of the enzyme induction in vivo but did not inhibit the activity in vitro. Addition of rifampicin to Sauton culture media resulted in a low enzyme level. Exogenous asparagine and glycerol were not indispensable for the enzyme synthesis and could be replaced by glutamate and glucose, respectively.
D001215:Asparaginase / Q000378:metabolism*; D004790:Enzyme Induction; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D009161:Mycobacterium / Q000201:enzymology*; D009168:Mycobacterium phlei / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development; D012293:Rifampin / Q000494:pharmacology
D001215:Asparaginase; D005947:Glucose; D012293:Rifampin