Psychrophilic, mesophilic, and thermophilic triosephosphate isomerases from three clostridial species.
Triosephosphate isomerase was purified to homogeneity as judged by analytical gel electrophoresis from clostridium sp. strain 69, clostridium pasteurianum, and C. thermosaccharolyticum, which grow optimally at 18, 37, and 55 C, respectively. Comparative studies on these purified proteins showed that they had the same molecular weight (53,000) and subunit molecular weight (26,500). They were equally susceptible to the active site-directed inhibitor, glycidol phosphate. However, their temperature and pH optima, as well as their stabilities to heat, urea, and sodium dodecyl sulfate, differed. The proteins also had different mobilities in acrylamide gel electrophoresis. This difference in ionic character was also reflected in the elution behavior of the enzymes from hydroxyapatite and in the isoelectric points determined by isoelectric focusing in acrylamide gel. The amino acid composition of these proteins showed that the thermophilic enzyme contains a greater amount of proline than the other enzymes. The ratio of acidic amino acids to basic amino acids was 1.79, 1.38, and 1.66 for the thermophilic mesophilic and psychrophilic enzymes, respectively. This is consistent with the relative isoelectric point values of these three enzymes.
D000596:Amino Acids / Q000032:analysis; D000645:Ammonium Sulfate; D000693:Anaerobiosis; D002238:Carbohydrate Epimerases / Q000032:analysis* / Q000378:metabolism; D011232:Chemical Precipitation; D002845:Chromatography; D002848:Chromatography, DEAE-Cellulose; D003013:Clostridium / Q000254:growth & development; D004589:Electrophoresis, Disc; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D006882:Hydroxyapatites; D007525:Isoelectric Focusing; D008970:Molecular Weight; D011489:Protein Denaturation; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013403:Sugar Phosphates / Q000494:pharmacology; D013696:Temperature; D014306:Trioses; D014508:Urea / Q000494:pharmacology
D000596:Amino Acids; D006882:Hydroxyapatites; D013403:Sugar Phosphates; D014306:Trioses; D012967:Sodium Dodecyl Sulfate; D014508:Urea; D002238:Carbohydrate Epimerases; D000645:Ammonium Sulfate