title: Electrolyte transport across a simple epithelium. Steady-state and transient analysis.
abstract: A simple transporting epithelium is represented as a cellular compartment, compliant in all dimensions, and a paracellular channel, of arbitrary shape, between well-stirred mucosal ans serosal baths. The equations for mass balance, Poiseuille flow, and the Nernst-Planck equation are used to describe the continuous behavior of the system along cell and channel, whereas passive transport across membranes is given by the relations of Kedem and Katchalsky. Time-dependent terms are retained to permit study of transient phenomena. Boundary conditions at the baths demand only mass conservation and specify no a priori estimates of the system variables. A numerical model containing Na+,K+,Cl-, and impermeant cellular anions is formulated with membrane parameters taken from the literature on Necturus gallbladder. The differential equations are represented as a finite difference scheme and solved using Newton's method. It appears that apical cellular NaCl cotransport is necessary to obtain a reasonable cell chloride concentration. Investigation of the osmolality of the transepithelial flow shows that at steady state a leaky epithelium cannot separate baths of substantially different tonicity, although this does not guarantee isotonic transport between equiosmolar media. Changes in bath pressure, application of transepithelial electrical potential, and simulation of ion-substitution experiments are performed to understand the role of membrane permeabilities in determining the dynamic behavior of the epithelium.
mesh_terms: D000818:Animals; D001693:Biological Transport, Active; D002462:Cell Membrane / Q000378:metabolism; D004553:Electric Conductivity; D004573:Electrolytes / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism*; D007473:Ion Channels / Q000378:metabolism*; D007700:Kinetics; D008433:Mathematics; D008954:Models, Biological
chemical_list: D004573:Electrolytes; D007473:Ion Channels