Antinociceptive action of oxotremorine and regional turnover of rat brain noradrenaline, dopamine and 5-HT.
In the rat, oxotremorine increases the threshold for vocalisation after-discharge (affective component of pain reactions) dose dependently at subtremor doses (30-67 mug/kg s.c.). Doses of 225-506 mug/kg were needed to elevate the thresholds for vocalisation and motor response. 1-Tryptophan, PCPA, alpha-methyl-p-tyrosine, 1-Dopa, pimozide and LSD-25 did not affect the antinociceptive activity of oxotremorine, while phenocybenzamine slightly increased the threshold for vocalisation. Oxotremorine did not change the endogenous brain concentrations of noradrenaline and dopamine or 5-HT but decreased that of 5-HIAA in all brain regions at the time of maximal analgesia. The decrease of 5-HIAA was still present after pretreatment with probenecid. After inhibition of tyrosine hydroxylase, oxotremorine accelerated the depletion of dopamine in telencephalic cortex during maximal antinociceptive activity and of noradrenaline in all brain regions at a time when this activity had vanished. Atropine significantly antagonized the analgesic activity of oxotremorine. It is concluded that oxotremorine antinociceptive activity in the rat is related to a cholinergic compoent, while a monoaminergic component is not directly involved.
D000700:Analgesics / Q000494:pharmacology*; D000818:Animals; D001285:Atropine / Q000494:pharmacology; D001921:Brain / Q000201:enzymology / Q000378:metabolism*; D001923:Brain Chemistry / Q000187:drug effects; D004298:Dopamine / Q000032:analysis / Q000378:metabolism*; D004558:Electric Stimulation; D006897:Hydroxyindoleacetic Acid / Q000032:analysis; D008297:Male; D008781:Methyltyrosines / Q000494:pharmacology; D009638:Norepinephrine / Q000032:analysis / Q000378:metabolism*; D010095:Oxotremorine / Q000494:pharmacology*; D011339:Probenecid / Q000494:pharmacology; D051381:Rats; D011930:Reaction Time / Q000187:drug effects; D012701:Serotonin / Q000032:analysis / Q000378:metabolism*; D014446:Tyrosine 3-Monooxygenase / Q000037:antagonists & inhibitors; D014828:Vocalization, Animal
D000700:Analgesics; D008781:Methyltyrosines; D012701:Serotonin; D006897:Hydroxyindoleacetic Acid; D010095:Oxotremorine; D001285:Atropine; D014446:Tyrosine 3-Monooxygenase; D011339:Probenecid; D004298:Dopamine; D009638:Norepinephrine