The permeability of rat liver lysosomes to sugars. Evidence for carrier-mediated facilitated diffusion.
1. By the osmotic-protection method, the penetration of sugars through the rat liver lysosomal membranes was studied with a view of determining whether sugar uptake was by facilitated diffusion. 2. The following criteria for this type of transport were established: sugar specificity, the order of uptake being 2-deoxy-D-glucose less than D-glucose less than D-mannose less than D-galactose less than D-ribose less than 2-deoxy-D-ribose; stereospecificity, the uptake of L-glucose and L-ribose being 50% slower than their D-stereoisomers; inhibition by 1 MM-phlorrhizin and 1 M-cytochalastin B; competition between sugars for uptake, and a Q10 (rate difference over a 10 degrees C temperature range) for uptake of approx. 2.8. 3. It is proposed that sugar uptake into lysosomes from rat liver is by facilitated diffusion.
D009597:4-Nitrophenylphosphatase / Q000378:metabolism; D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects; D050260:Carbohydrate Metabolism; D003571:Cytochalasin B / Q000494:pharmacology; D004058:Diffusion; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008247:Lysosomes / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D009995:Osmosis; D010695:Phlorhizin / Q000494:pharmacology; D051381:Rats; D013237:Stereoisomerism; D013696:Temperature
D003571:Cytochalasin B; D010695:Phlorhizin; D009597:4-Nitrophenylphosphatase