[A new method for determination of ATPase activity in tissue homogenates].
Authors describe a new method to determine Mg2+ and Na+K+ATPase activity on extremely small samples of tissue homogenates. They use specific inhibitors to discern between the two activities and a sensitive colorimetric method to dose Pi released from ATP, Lower limit of sensitivity is about 0,0100 micromoles of Pi/mg proteins/hour.
D000251:Adenosine Triphosphatases / Q000032:analysis*; D000818:Animals; D001921:Brain / Q000201:enzymology; D004796:Clinical Enzyme Tests / Q000379:methods*; D003124:Colorimetry / Q000379:methods*; D006801:Humans; D007678:Kidney Glomerulus / Q000201:enzymology; D008099:Liver / Q000201:enzymology; D009132:Muscles / Q000201:enzymology; D010710:Phosphates / Q000032:analysis; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000032:analysis
D010710:Phosphates; D000251:Adenosine Triphosphatases; D000254:Sodium-Potassium-Exchanging ATPase
Un nuovo metodo per il dosaggio dell'attività ATPasica in omogenati di tessuto.