title: Studies on the cholesterol synthesis in the human adipose tissue. II. Mechanism of metabolic shifts and regulation of cholesterol through ascorbic acid.
abstract: Ratios of yields of cholesterol in different media with different metabolites (200 estimations) show that if the initial normal quantity in a system is X, glucose makes it 4X which is then reduced by the amino acids and ascorbic acid. Alanine, Serine, Threonine, Cysteine, Cystine and Lysine reduce it to 2X. Glycine, Valine, Leucine, Aspartic, Phenylalanine, Tyrosine, Tryptophan roughly increase it to 6X. Other amino acids are intermediate between the above sets. Balanced amino acids and fatty acids keep up the glucose value 4X but the individual amino acids behave as above. Ascorbic acid reduces the quantity of cholesterol everywhere from one third to one half bringing it back to normal X. Since it is not directly involved in the cholesterol synthesis, it must be acting through the pyridine nucleotides. The interesting feature is that the C/H ratio in the amino acids  corresponds to the yield of cholesterol with respect to glucose saline yield = 1. Mechanism of metabolic shifts has been discussed on the empirical basis as also on the basis of the change in ratios of NAD+ (NADP+) to NADH2 (NADPH2) through ascorbic acid.
mesh_terms: D000085:Acetates / Q000378:metabolism; D000273:Adipose Tissue / Q000378:metabolism*; D000596:Amino Acids / Q000378:metabolism; D001205:Ascorbic Acid / Q000378:metabolism*; D002784:Cholesterol / Q000096:biosynthesis*; D005260:Female; D005947:Glucose / Q000378:metabolism; D006801:Humans; D009243:NAD / Q000378:metabolism; D010168:Palmitates / Q000378:metabolism
chemical_list: D000085:Acetates; D000596:Amino Acids; D010168:Palmitates; D009243:NAD; D002784:Cholesterol; D005947:Glucose; D001205:Ascorbic Acid