title: The uptake of L-glutamate by the central nervous system of the cockroach, Periplaneta americana.
abstract: A concentrative uptake mechanism for L-glutamate with the following characteristics has been identified in the abdominal nerve cord: 1. The uptake can be divided into Na+-sensitive and Na-plus-insensitive components. 2. The Na-plus-sensitive component showed the typical saturation kinetics of a carrier mediate process. It had a V of 15.9 x 10(6) times 10(6) muM/mg wet weight/min and a Km of 0-33 mm. Its magnitude was proportional to the first power of the Na-plus concentration of the medium. The uptake was specific for L-dicarboxylic amino acids and was sensitive to the presence of metabolic inhibitors. 3. The Na-plus-insensitive component was linearly related to the glutamate concentration of the medium. An isosmotic saline is described for use with the isolated intact abdominal nerve cord of P. americana.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001812:Blood-Brain Barrier; D002490:Central Nervous System / Q000378:metabolism*; D003058:Cockroaches / Q000033:anatomy & histology / Q000378:metabolism*; D018691:Excitatory Amino Acid Antagonists; D005971:Glutamates / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D012964:Sodium / Q000378:metabolism; D012965:Sodium Chloride
chemical_list: D000596:Amino Acids; D018691:Excitatory Amino Acid Antagonists; D005971:Glutamates; D012965:Sodium Chloride; D012964:Sodium