Characteristics of Sendai virus RNA transcriptive complex formed in the cytoplasm of infected ascites cells.
Virus-specific structures with sedimentation coefficients of 250-300, 200 and 150 S were isolated from the polysome fraction of Sendai virus-infected Ahrlich ascitic carcinoma cells treated with cycloheximide, at early stages of infection (1.5 to 2 hours after inoculation). All these 3 types of structure contained both parental and newly synthesized viral RNA. RNA extracted from these structures consisted of 2 components sedimenting in sucrose density gradients in the zones of 50-70 and 35-40 S. Both components contained parental and newly synthesized RNA and were partially resistant to ribonuclease. RNA extracted from rapidly sedimenting structures (250-300 S) contained mainly the 50-70 S component; RNA recovered from 200 S structures contained the 35-40 S component. By analogy with reported data, the isolated forms of RNA have been characterized as transcriptive intermediates.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D046508:Culture Techniques; D003513:Cycloheximide / Q000494:pharmacology; D003593:Cytoplasm / Q000378:metabolism; D010222:Parainfluenza Virus 1, Human / Q000032:analysis / Q000378:metabolism*; D011132:Polyribosomes / Q000378:metabolism; D012367:RNA, Viral / Q000032:analysis / Q000096:biosynthesis; D012260:Ribonucleases / Q000378:metabolism; D014158:Transcription, Genetic
D012367:RNA, Viral; D003513:Cycloheximide; D012260:Ribonucleases