Periodate or glutaraldehyde for preparing peroxidase conjugates?
Conjugates of horseradish peroxidase with antibodies (anti-human IgG (H + L)) or their Fab' fragments were prepared according to the newest modification of the periodate (P-) method or the two-step glutaraldehyde (G-) method. The conjugates were analysed by gel chromatography and subsequently tested in three different applications. For tissue immunohistochemistry on sections of lupus erythematosus skin, G-conjugates were preferred to polymeric P-conjugates. In ELISA for detection of human antibodies against penicillin P-conjugates were superior to G-conjugates. For the detection of surface Ig on lymphoid cells both types of conjugate were more or less equally suitable. A scheme for the most suitable combinations of method of preparation and field of application is given.
D000447:Aldehydes / Q000494:pharmacology*; D000818:Animals; D000906:Antibodies; D000954:Antigens, Surface; D004797:Enzyme-Linked Immunosorbent Assay; D005455:Fluorescent Antibody Technique; D005976:Glutaral / Q000494:pharmacology*; D006651:Histocytochemistry; D006735:Horseradish Peroxidase; D006801:Humans; D007140:Immunoglobulin Fab Fragments; D010504:Periodic Acid / Q000494:pharmacology*; D011817:Rabbits
D000447:Aldehydes; D000906:Antibodies; D000954:Antigens, Surface; D007140:Immunoglobulin Fab Fragments; D010504:Periodic Acid; D006735:Horseradish Peroxidase; D005976:Glutaral