The immunochemistry of (L-Ala-D-Glu-L-Lys-D-Ala-Gly)n: II. Rabbit antibodies of restricted heterogeneity.
A rabbit antiserum of restricted specificity elicited by the sequential polypeptide, (L-Ala-D-Glu-L-Lys-D-Ala-Gly)n, was purified by column chromatography using Sepharose 4B to which the inhibitor, Gly-L-Ala-D-Glu-Gly, was covalently attached. Approximately 95% of the antibody activity was retained on the immunoabsorbant. After elution, the purified antibody was shown by electrofocusing in a sucrose gradient to be of restricted heterogeneity. The entire antibody fraction was contained within the pH range of 6.9-8.9. The normal immunoglobulin pool from this rabbit, on the other hand, was found by electrofocusing to be distributed throughout the pH range studied (3.6-9.2).
D000042:Absorption; D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D000910:Antibodies, Heterophile; D000918:Antibody Specificity; D006863:Hydrogen-Ion Concentration; D007158:Immunologic Techniques; D007525:Isoelectric Focusing; D010455:Peptides; D011817:Rabbits / Q000276:immunology
D000906:Antibodies; D000910:Antibodies, Heterophile; D010455:Peptides