A rapid and specific method for separation of bound and free antigen in radioimmunoassay systems.
This communication reports a method for increasing the speed of separation of bound and free antigen in radioimmunoassay systems with no loss in the specificity of binding. The technique uses a mixture of second antibody and polyethylene glycol. It is not species or antibody specific, and systems using specific first antibodies from rabbit, goat or sheep are all functional. Results for the assay of parathyrin, calcitonin and corticotropin are described here, although the system has been shown to work for triiodothyronine, thyroxin, thyrotropin, thyroxine binding globulin and transferrin. The time taken for the reaction between first and second antibody is in the order of seconds, and the stability of the complex is unchanged over a period of hours.
D000324:Adrenocorticotropic Hormone / Q000032:analysis*; D000941:Antigens / Q000032:analysis*; D001798:Blood Proteins / Q000032:analysis; D002116:Calcitonin / Q000032:analysis*; D006728:Hormones / Q000032:analysis; D007700:Kinetics; D010281:Parathyroid Hormone / Q000032:analysis*; D011092:Polyethylene Glycols; D011485:Protein Binding; D011863:Radioimmunoassay / Q000379:methods
D000941:Antigens; D001798:Blood Proteins; D006728:Hormones; D010281:Parathyroid Hormone; D011092:Polyethylene Glycols; D000324:Adrenocorticotropic Hormone; D002116:Calcitonin