Metabolism of D-mannosamine in bovine thyroid gland slices.
1. The nature of the acid-soluble mannosamine intermediates was investigated in bovine thyroid gland slices after incubation with a tracer amount of [14C]mannosamine plus 10 mM carrier mannosamine. 2. These radioactive intermediates were separated by ion exchange, columns, characterized by paper chromatography and were identified as N-acetylmannosamine-6-P. The radioactivity associated with these acid-soluble compounds constituted about 17 percent of the added [14C]mannosamine. 3. [14C]Mannosamine was incorporated as sialic acid only into thyroglobulin.
D000085:Acetates / Q000378:metabolism; D000818:Animals; D002417:Cattle; D006595:Hexosamines / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008358:Mannose / Q000378:metabolism; D009438:Neuraminic Acids / Q000378:metabolism; D012995:Solubility; D013403:Sugar Phosphates / Q000378:metabolism; D013954:Thyroglobulin / Q000096:biosynthesis; D013961:Thyroid Gland / Q000378:metabolism*; D013997:Time Factors; D014238:Trichloroacetic Acid
D000085:Acetates; D006595:Hexosamines; D009438:Neuraminic Acids; D013403:Sugar Phosphates; D014238:Trichloroacetic Acid; D013954:Thyroglobulin; D008358:Mannose