title: The in vitro effects of Bordetella pertussis lymphocytosis-promoting factor on murine lymphocytes. III. B-cell dependence for T-cell proliferation.
abstract: The nature of the helper lymphocytes in lymphocytosis-promoting factor (LPF)-induced proliferation was explored. Removal of macrophages from adherent splenocytes by either carbonyl-iron incubation or passage through Sephadex G-10 columns did not affect their synergistic function. Nor did cytolysis with Thy-1.2 antiserum and complement. The helper cells were found to be surface immunoglobulin-positive (sIg+) because they are retained by anti-Ig columns, susceptible to lysis by rabbit anti-mouse immunoglobulin and complement, and occurred in the sIg+ fractions of splenocytes after separation on the fluorescence-activated cell sorter. Further delineation of the surface markers on helper cells showed that complement receptors are not the determining marker for synergistic function. The requirement for B-helper cells in the stimulation of T lymphocytes by LPF is unique for a mouse of T-cell mitogen.
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D000961:Antilymphocyte Serum / Q000494:pharmacology; D001402:B-Lymphocytes / Q000502:physiology*; D001886:Bordetella pertussis; D002455:Cell Division; D003165:Complement System Proteins; D005260:Female; D007376:Interleukin-2; D008222:Lymphokines; D008264:Macrophages / Q000502:physiology; D051379:Mice; D008808:Mice, Inbred CBA; D011947:Receptors, Antigen, B-Cell; D011955:Receptors, Drug; D013601:T-Lymphocytes / Q000166:cytology*
chemical_list: D000888:Antibodies, Anti-Idiotypic; D000961:Antilymphocyte Serum; D007376:Interleukin-2; D008222:Lymphokines; D011947:Receptors, Antigen, B-Cell; D011955:Receptors, Drug; D003165:Complement System Proteins