Determination of high-density lipoproteins: screening methods compared.
Factors reflecting the concentration of high-density lipoproteins in serum were assessed for 108 men and 106 women participating in a Venetian screening program for hyperlipoproteinemia. The methods applied, optimized in our laboratory, were: (a) cholesterol in high-density lipoproteins, determined in the supernate after sedimentation of the very-low-density lipoproteins + low-density lipoproteins with dextran sulfate or sodium phosphotungstate; and (b) immunochemical quantitation of apolipoprotein A-I and apolipoprotein A-II by Laurell's "rocket" technique. The latter determinations were performed with total serum before and after delipidation with diispropyl ether/n-butanol (6/4 by vol). The dextran sulfate method gave about 5% higher values than did the phosphotungstate method, but the correlation between the two was excellent (r = 0.95). Results of the immunochemical quantitation indicate that delipidation of lipoproteins before Laurell electrophoresis may not be necessary if only freshly drawn sera are used.
D000328:Adult; D001053:Apolipoproteins / Q000097:blood; D005260:Female; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood / Q000175:diagnosis; D007122:Immunoelectrophoresis / Q000379:methods; D008075:Lipoproteins, HDL / Q000097:blood*; D008297:Male; D008403:Mass Screening; D008875:Middle Aged; D012016:Reference Values; D012737:Sex Factors
D001053:Apolipoproteins; D008075:Lipoproteins, HDL