Transformation of hamster embryonic fibroblast cells by UV-irradiated human cytomegalovirus.
Infection of hamster embryonic fibroblast (HEF) cells with UV-irradiated human CMV resulted in appearance of transformed clones. The established cell line (87-TRH-5) when inoculated subcutaneously induced tumour in newborn hamsters. The 87-TRH-5 cell line and the cell lines (87-TRH-5-Tsc1, 87-TRH-5-Tsc2, 87TRH-5-Tsc3) developed from the induced tumour, proved to bear CMV-spcific cytoplasmic and surface antigens. Demonstration of infectious CMV or MCV-specific nuclear antigen in 87-THR-5-cells has failed.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D006224:Cricetinae; D003587:Cytomegalovirus / Q000276:immunology / Q000502:physiology*; D005347:Fibroblasts; D009374:Neoplasms, Experimental / Q000209:etiology; D014466:Ultraviolet Rays
D000956:Antigens, Viral