title: Endogenous purine metabolism in the conidia of wild type and certain adenine mutants of Neurospora crassa. I. The nature of the reserve pools and pool utilization during adenine starvation.
abstract: Conidia of four adenine auxotrophs (ad 9, ad 3B, ad 8 and ad 4) of Neurospora crassa differ in their ability to germinate on adenine-deficient medium. A large percentage of the ad 9 and ad 3B mutant conidia germinate while those of ad 8 and ad 4 mutant do not. No correlation was found between the size of the conidial purine reserves and the conidial ability to germinate. In all the strains the major fraction of the conidial purine reserve pools was inosine. The ad 8 and ad 4 mutants are blocked after IMP formation in the adenine biosynthetic pathway and therefore cannot use the stored inosine for germination. Pool-utilization studies indicated that in all strains investigated some of the purine reserves were lost from the conidia during incubation. In the most readily germinating strain, ad 9, only small amounts of the purine pool were lost from the conidia and a large portion of the reserve pool was used for nucleic acid synthesis. The nature of the purine reserves present in the conidia, and the ability of the strains to prevent loss of the stored purines from the conidia appear to be among the factors influencing the conidial germination of the adenine mutants of N. crassa.
mesh_terms: D000225:Adenine / Q000172:deficiency / Q000378:metabolism; D000483:Alleles; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D007288:Inosine / Q000378:metabolism; D009154:Mutation; D009491:Neurospora / Q000378:metabolism*; D009492:Neurospora crassa / Q000254:growth & development / Q000378:metabolism*; D009696:Nucleic Acids / Q000096:biosynthesis; D009711:Nucleotides / Q000378:metabolism; D011687:Purines / Q000378:metabolism*; D012995:Solubility; D013045:Species Specificity
chemical_list: D003470:Culture Media; D009696:Nucleic Acids; D009711:Nucleotides; D011687:Purines; D007288:Inosine; D000225:Adenine