Enhancement of 2-acetylaminofluorene liver carcinogenesis in rats fed a choline-devoid diet.
The effects of feeding a choline-devoid (CD) or a choline-supplemented (CS) diet on the induction of liver tumors in rats by 2-acetylaminofluorene (AAF) were investigated. Male Sprague-Dawley rats were fed either a CD or a CS diet containing 0.0075% AAF. Three to eight animals were killed 1, 3, 4 and 6 months thereafter. Well to moderately well differentiated hepatocellular carcinomas developed in 50% and 75% of rats fed the CD + AAF diet for 4 and 6 months, respectively. Cholangiocarcinomas developed, after 6 months, in 38% of the animals. No tumor developed in rats fed the CS + AAF diet. The results extend to AAF those previously obtained with DL-ethionine and azaserine showing that feeding a CD diet greatly potentiates the induction in rats of liver tumors by chemical carcinogens.
D015073:2-Acetylaminofluorene; D002759:Adenoma, Bile Duct / Q000139:chemically induced* / Q000473:pathology; D000818:Animals; D001650:Bile Duct Neoplasms / Q000139:chemically induced* / Q000473:pathology; D006528:Carcinoma, Hepatocellular / Q000139:chemically induced* / Q000473:pathology; D002796:Choline Deficiency / Q000150:complications*; D005449:Fluorenes; D008113:Liver Neoplasms / Q000139:chemically induced* / Q000473:pathology; D008297:Male; D009374:Neoplasms, Experimental / Q000139:chemically induced / Q000473:pathology; D051381:Rats
D005449:Fluorenes; D015073:2-Acetylaminofluorene