title: The physical and genetic organization of a viral genome.
abstract: Mutants of SV40 with deletions of a few to several thousand base pairs have been constructed in vitro and cloned in cultured monkey cells. The location and size of these deletions has been determined by restriction endonuclease mapping and electron microscopic and enzymatic analysis of DNA heteroduplex molecules. Analysis of the phenotype of these deletion mutants permits us to specify the locations of the known SV40 genes, in particular, the novel organization of SV40s two early genes that are required for oncogenesis.
mesh_terms: D000956:Antigens, Viral; D001482:Base Composition; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000378:metabolism; D005814:Genes, Viral; D009154:Mutation; D013539:Simian virus 40 / Q000378:metabolism*; D014764:Viral Proteins / Q000096:biosynthesis
chemical_list: D000956:Antigens, Viral; D004279:DNA, Viral; D014764:Viral Proteins; D004262:DNA Restriction Enzymes