Transfection of human lymphoblastoid cells with herpes simplex viral DNA.
The "calcium/dimethyl sulfoxide shock" method of transfection was adapted for use in human lymphoid cell cultures. One microgram of herpes simplex virus type 1 DNA regularly initiated virus replication in four lymphoblastoid cell lines. Per 10(5) cells exposed to 1 microgram of DNA, 0.5--5 cells formed an infectious center. The minimal infective dose of DNA was approximately 500 ng.
D002460:Cell Line; D004279:DNA, Viral / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism* / Q000382:microbiology; D018139:Simplexvirus / Q000235:genetics*; D014162:Transfection; D014779:Virus Replication
D004279:DNA, Viral