New genes and promoters suggested by the DNA sequence near the end of the coliphage T7 early operon.
We have employed the dideoxynucleotide chain-terminating method to determine the nucleotide sequence of T7 DNA between the physical map positions 18.9% and 19.8%. The most striking features of this sequence are two perfect 21-basepair repeats, each of which appears to contain a promoter for late transcription. In each case the promoter sequence incorporates a putative translational terminator on its left (5'-side of the "sense" strand), and overlaps a potential ribosome-binding site on its right. The region probably lies immediately distal to the early operon, and may contain two short, hitherto unreported protein-coding sequences.
D001483:Base Sequence; D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000235:genetics*; D005814:Genes, Viral; D009876:Operon; D014176:Protein Biosynthesis; D013604:T-Phages / Q000235:genetics*; D013997:Time Factors; D014158:Transcription, Genetic
D004279:DNA, Viral; D004262:DNA Restriction Enzymes