Vesicular stomatitis virus glycoprotein is anchored to intracellular membranes near its carboxyl end and is proteolytically cleaved at its amino terminus.
The intracellular vesicular stomatitis virus glycoprotein (G) is inserted into membranes such that a small portion of one end of the molecule is exposed on the cytoplasmic surface of the endoplasmic reticulum and is susceptible to proteolytic digestion (T.G. Morrison, C.O. McQuain, and D. Simpson, J. Virol. 28:368-374). We have determined that this region of the G protein contains two methionyl tryptic peptides. The methionyl tryptic peptides of the G protein have been ordered by the use of the antibiotic pactamycin, and the two methionyl tryptic peptides removed by proteolytic digestion of intracellular G protein have been shown to be derived from the carboxyl terminal end of the protein. In addition, we have found that the unglycosylated G protein synthesized in a reticulocyte cell-free reaction migrates on polyacrylamide gels slightly slower than the unglycosylated G protein synthesized in tunicamycin-treated infected cells. We have also compared these G proteins derived from different sources by partial proteolysis (D.W. Cleveland, S.G. Fischer, M.W. Kirschner, and V.K. Laemmli, J. Biol. Chem. 252:1102-1106) and by chymotryptic peptide analysis. We have found minor differences between the two proteins consistent with the removal of 10 to 15 amino acids from the amino terminus of the intracellular G protein.
D002460:Cell Line; D002918:Chymotrypsin / Q000378:metabolism; D006023:Glycoproteins / Q000032:analysis*; D007425:Intracellular Membranes / Q000032:analysis; D008715:Methionine / Q000032:analysis; D010455:Peptides / Q000032:analysis; D014357:Trypsin / Q000378:metabolism; D014721:Vesicular stomatitis Indiana virus / Q000032:analysis*; D014764:Viral Proteins / Q000032:analysis*
D006023:Glycoproteins; D010455:Peptides; D014764:Viral Proteins; D008715:Methionine; D002918:Chymotrypsin; D014357:Trypsin