title: The role of various prostaglandins on the correlation between permeability to albumin and cAMP levels in the isolated mesentery.
abstract: Prostaglandins (PG) have been shown to raise the level of cyclic AMP (cAMP) in various tissues, and to increase permeability. Whether both events are linked, is at present a matter of speculation. We have investigated the effects of PGE1, E2, A1, A2, F1alpha and F2alpha on an isolated rat mesentery placed in a diffusion cell (surface area : 2 sq.cm). The PGs (5 microgram/ml) increased the passage of (I 125) - Albumin across the mesentery. In other experiments, diks of rat mesentery (surface area : 2 sq.cm) have been incubated in assay tubes, and cAMP levels measured by a binding protein assay. We have observed an excellent correlation between increases in permeability and cAMP levels (r=0.961). In order of increasing potency on both parameters, the PGs may be classified as follows : PGF, PGA and PGE. In the rat mesentery, under the influence of prostaglandins, increases in permeability and in cAMP levels are apparently connected.
mesh_terms: D000418:Albumins / Q000378:metabolism*; D000818:Animals; D002199:Capillary Permeability / Q000187:drug effects; D002463:Cell Membrane Permeability / Q000187:drug effects*; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D008643:Mesentery / Q000166:cytology / Q000378:metabolism*; D011455:Prostaglandins A, Synthetic / Q000494:pharmacology; D011459:Prostaglandins E, Synthetic / Q000494:pharmacology; D011461:Prostaglandins F, Synthetic / Q000494:pharmacology; D011465:Prostaglandins, Synthetic / Q000494:pharmacology*; D011485:Protein Binding; D051381:Rats; D012711:Serum Albumin, Radio-Iodinated
chemical_list: D000418:Albumins; D011455:Prostaglandins A, Synthetic; D011459:Prostaglandins E, Synthetic; D011461:Prostaglandins F, Synthetic; D011465:Prostaglandins, Synthetic; D012711:Serum Albumin, Radio-Iodinated; D000242:Cyclic AMP