Molecular cloning of the restriction fragments derived from double EcoRI/PstI digestion of the calf satellite I DNA and their restriction analysis.
Calf satellite I DNA digested with EcoRI and PstI gives three fragments 643, 621 and 83 bp long. Two of them, the 643 and 621 bp were cloned using pBR 322 vector and analyzed by means of the MspI and HaeIII restriction enzymes.
D000818:Animals; D002417:Cattle; D003001:Cloning, Molecular; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000378:metabolism; D004276:DNA, Satellite / Q000378:metabolism; D015252:Deoxyribonucleases, Type II Site-Specific; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight
D004274:DNA, Recombinant; D004276:DNA, Satellite; D004262:DNA Restriction Enzymes; C024388:CTGCAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific