Intermediates and enzymes between alpha-ketoarginine and gamma-guanidinobutyrate in the L-arginine catabolic pathway of Pseudomonas putida.
In Pseudomonas putida P2 grown on L-arginine as the sole source of carbon and nitrogen, catabolism of L-arginine forms of alpha-ketoarginine, gamma-guanidinobutyrate, and gamma-aminobutyrate. A previously undetected intermediate, gamma-guanidinobutyraldehyde, is identified as the product of alpha-ketoarginine decarboxylase. An 86-fold purification of this enzyme is described. Activity is thiamine pyrophosphate-dependent and cofactor reassociation is facilitated by divalent cations. The order of effectiveness is Mn-2+ greater than Mg-2+, Co-2+ greater than Ca-2+ greater than Ni-2+ greater than Zn-2+. An inducible enzyme that catalyzes conversion of gamma-guanidinobutyraldehyde to gamma-guanidinobutyrate has been studied in cell-free extracts. NAD-+, but no other cofactors, is required. By differential nutritional growth experiments, 4 regulatory units for the L-arginine pathway are proposed and inducers of 2 units are identified.
D000445:Aldehyde Oxidoreductases / Q000378:metabolism; D000447:Aldehydes / Q000378:metabolism; D000613:Aminobutyrates / Q000378:metabolism; D000641:Ammonia / Q000378:metabolism; D001120:Arginine / Q000031:analogs & derivatives / Q000378:metabolism*; D002087:Butyrates / Q000378:metabolism*; D002262:Carboxy-Lyases / Q000302:isolation & purification* / Q000378:metabolism; D002413:Cations, Divalent; D002854:Chromatography, Paper; D002855:Chromatography, Thin Layer; D004591:Electrophoresis, Polyacrylamide Gel; D004790:Enzyme Induction; D005971:Glutamates / Q000378:metabolism; D006146:Guanidines / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007651:Keto Acids / Q000378:metabolism*; D007700:Kinetics; D009243:NAD / Q000378:metabolism; D011549:Pseudomonas / Q000378:metabolism*; D013386:Succinates / Q000378:metabolism; D013696:Temperature; D013835:Thiamine Pyrophosphate / Q000494:pharmacology
D000447:Aldehydes; D000613:Aminobutyrates; D002087:Butyrates; D002413:Cations, Divalent; D005971:Glutamates; D006146:Guanidines; D007651:Keto Acids; D013386:Succinates; D009243:NAD; D000641:Ammonia; D001120:Arginine; D000445:Aldehyde Oxidoreductases; D002262:Carboxy-Lyases; C021465:alpha-ketoarginine decarboxylase; D013835:Thiamine Pyrophosphate