title: Physical characterization of deoxyribonucleic acids of different human papilloma viruses (HPV).
abstract: Different types of human papilloma viruses (HPV) had been identified by the use of bacterial restriction endonucleases (Gissmann et al., 1977). The DNAs of HPV 1, 2, and 3 showing a similar restriction enzyme pattern can be purified in sufficient quantities to permit further characterization. These viruses appear not to be identical, as far as molecular weight (4.9 X 10(6) d), buoyant density (1.700 mu/ml), and sedimentation values of the different DNA components (Co I: 22--23S; Co II: 17S; Co III: 16S) are concerned. HPV 4 DNA, which is entirely different from HPV 1--3 in its restriction enzyme pattern, does not hybridize with cRNA transcribed in vitro from HPV 1 DNA. The cRNA was shown to represent the whole viral genome.
mesh_terms: D002499:Centrifugation, Density Gradient; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004270:DNA, Circular / Q000032:analysis*; D004279:DNA, Viral / Q000032:analysis*; D006801:Humans; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D027383:Papillomaviridae / Q000032:analysis*; D014860:Warts / Q000382:microbiology*
chemical_list: D004270:DNA, Circular; D004279:DNA, Viral; D004262:DNA Restriction Enzymes