[Nonspecific means of increasing the sensitivity of the passive hemagglutination reaction].
The effect of some methods of preliminary treatment of erythrocytes on the PHAT depended on the sensitin náture and the method of erythrocyte load. In case of erythrocyte load with nonprotein and immunoglobulin sensitins without any conjugating agents the simulating effect of heating and periodate treatment was caused not by increase of stable sensitin binding, but by the reduction of physico-chemical resistance of erythrocytes. This effect of erythrocyte treatment permitted to increase the sensitivity of the antibodies and antigens determination. In loading the erythrocytes with the aid of conjugating agents and in sensitization with protein antigens after Boyden no stimuating effect of the treatment was noted.
D000818:Animals; D000937:Antigen-Antibody Reactions / Q000187:drug effects; D000942:Antigens, Bacterial / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D004912:Erythrocytes / Q000276:immunology; D005615:Freezing; D006386:Hemagglutination Tests / Q000379:methods*; D007106:Immune Sera / Q000276:immunology; D007114:Immunization; D010504:Periodic Acid / Q000276:immunology; D013696:Temperature
D000942:Antigens, Bacterial; D007106:Immune Sera; D010504:Periodic Acid
Nespetsificheskie sposoby povysheniia chuvstvitel'nosti reaktsii passivnoĭ gemaggliutinatsii.