The beta-adrenergic responses in isolated splenic capsule area of several species of animals.
In isolated splenic capsules of mouse, rat, guinea pig, rabbit, cat and dog, either a single or a cumulative dose of l- and dl-isoproterenol at a concentration higher than l0(-9) M, induced a relaxation which was inhibited or blocked with pretreatment of 10(-5) M propranolol. At a concentration of 5 x 10(-6) M to 10(-5) M 1- and dl-isoproterenol, an inverse contraction was induced, and such response was prevented or reversed to one of relaxation in the presence of 10(-5) M phentolamine. Application of 10(-5) M epinephrine produced a strong contraction and such was reversed to one of relaxation after addition of 10(-4) M phentolamine. This relaxation was reduced or blocked with pretreatment of 10(-5) M propranolol. Thus, it was confirmed that both alpha- and beta-receptors exist in the splenic capsule area of these different species.
D000818:Animals; D002415:Cats; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D004837:Epinephrine / Q000037:antagonists & inhibitors; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007545:Isoproterenol / Q000037:antagonists & inhibitors / Q000494:pharmacology; D051379:Mice; D009119:Muscle Contraction / Q000187:drug effects; D009126:Muscle Relaxation / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects; D010646:Phentolamine / Q000494:pharmacology; D011433:Propranolol / Q000494:pharmacology; D011817:Rabbits; D051381:Rats; D011941:Receptors, Adrenergic / Q000032:analysis*; D011942:Receptors, Adrenergic, alpha / Q000032:analysis; D011943:Receptors, Adrenergic, beta / Q000032:analysis*; D013045:Species Specificity; D013154:Spleen / Q000032:analysis*; D013552:Swine
D011941:Receptors, Adrenergic; D011942:Receptors, Adrenergic, alpha; D011943:Receptors, Adrenergic, beta; D011433:Propranolol; D007545:Isoproterenol; D004837:Epinephrine; D010646:Phentolamine