title: State and reactivity of tryptophyl residues in two bacterial proteases from Sorangium sp.
abstract: The state and reactivity of tryptophyl residues in two proteolytic enzymes from Sorangium sp. were investigated by means of the following methods: spectrophotometric oxidation of tryptophans with N-bromosuccinimide, 2-hydroxy-5-nitrobenzyl bromide, and H2O2 in dioxane, optical rotatory dispersion, ultraviolet difference spectrophotometry, solvent perturbation and viscosity measurements. Out of two tryptophyl residues/molecule of alpha-lytic protease, one appears to be completely buried, while the other seems to be exposed. None of these two residues seem to be responsible for the activity of the enzyme. The beta-lytic protease undergoes an irreversible conformational transition between pH 5.0 and 3.5. Out of total four tryptophyl residues/molecule, only one is fully exposed at neutral pH. The other three are gradually exposed in the pH transition region. The degree of exposure and the dimensions of "cavities" shielding tryptophyl residues were estimated. The tryptophyl residues of of beta-lytic protease do not seem to participate in substrate binding or the active site; they are rather one of the determinants of the conformational state of the enzyme.
mesh_terms: D015077:2-Hydroxy-5-nitrobenzyl Bromide / Q000494:pharmacology; D001665:Binding Sites; D001974:Bromosuccinimide / Q000494:pharmacology; D004146:Dioxanes / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D009231:Myxococcales / Q000201:enzymology*; D009905:Optical Rotatory Dispersion; D010084:Oxidation-Reduction; D010447:Peptide Hydrolases / Q000032:analysis*; D011487:Protein Conformation; D012997:Solvents / Q000494:pharmacology; D013056:Spectrophotometry, Ultraviolet; D014364:Tryptophan / Q000032:analysis*; D014783:Viscosity
chemical_list: D004146:Dioxanes; D012997:Solvents; D015077:2-Hydroxy-5-nitrobenzyl Bromide; D014364:Tryptophan; D006861:Hydrogen Peroxide; D010447:Peptide Hydrolases; D001974:Bromosuccinimide