The possible relationship between a membrane conformational change and photosystem II dependent hydrogen ion accumulation and adenosine 5'-triphosphate synthesis.
Data are presented which suggest that photosystem II dependent hydrogen ion accumulation and ATP synthesis can occur only after the lamellar membranes have undergone a conformational change. This membrane conformational change is detected by the electron transport dependent incorporation of diazonium benzene[35S]sulfonate into membrane components. Previously it was established that electron flux from the photosystem II primary acceptor to plastoquinone is a necessary event for the occurrence of the diazonium-detected conformational change. These studies indicate that the release of hydrogen ions during photosystem II oxidation of the primary reductant is also a necessary event for the diazonium-detected conformational change. When iodide were substituted for water (or other proton-releasing donors) as the primary reductant of system II the conformational change did not occur even though a substantial rate of electron flow from the primary acceptor to plastoquinone occurred.
D000255:Adenosine Triphosphate / Q000378:metabolism*; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D002736:Chloroplasts / Q000187:drug effects / Q000378:metabolism*; D003486:Cyanides / Q000494:pharmacology; D004237:Diuron / Q000494:pharmacology; D004579:Electron Transport; D006863:Hydrogen-Ion Concentration; D008027:Light; D010785:Photophosphorylation / Q000187:drug effects; D010944:Plants
D003486:Cyanides; D000255:Adenosine Triphosphate; D004237:Diuron