Production and purification of the thermophilic bacteriophage TP-84.
A new procedure for production and purification of the thermophilic bacteriophage TP-84 in high yields is described. Cultures of Bacillus stearothermophilus strain 10, enriched with nutrients to obtain heavy growth and to prevent sporulation and maintained at a pH of 6.5, were infected with the phage in a 100-liter fermentor.  Addition of magnesium chloride (0.01 M) and a temperature of 58-C were essential for maximal phage production.  Phage (5 times 1011 infective particles/ml) was precipitated with polyethylene glycol (molecular weight 6,000) in the presence of sodium chloride and was further purified by cesium chloride density centrifugation.
D001407:Bacillus / Q000254:growth & development; D001435:Bacteriophages / Q000254:growth & development* / Q000302:isolation & purification; D002452:Cell Count; D002499:Centrifugation, Density Gradient; D011232:Chemical Precipitation; D003470:Culture Media; D005632:Fructose; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000494:pharmacology; D011092:Polyethylene Glycols; D012965:Sodium Chloride / Q000494:pharmacology; D010948:Viral Plaque Assay; D014779:Virus Replication / Q000187:drug effects
D003470:Culture Media; D005632:Fructose; D011092:Polyethylene Glycols; D012965:Sodium Chloride; D008274:Magnesium