[A receptor for UDP-glucose in the microsomal membranes of rat hepatocytes].
Rat liver microsomal membranes have been shown to contain a biosynthetic pathway of UDP-glucose. In addition, they are able to bind UDP-glucose in a reversible manner, As UDP-glucose is also metabolized in these membranes, the study of the binding has been performed with a microsomal Triton X 100 extract. This reversible binding depends on pH (maximum at pH 8.1) and manganous ions, and disappears at pH 6.5. It exhibits a high affinity (K-diss equals 3 mu M), and a narrow specifity for UDP-glucose. Proteolytic digestion inhibits the binding up to 90%, showing that the UDP-glucose receptor has a proteic nature. These binding characteristics have been also found in the membranes themselves, indicating that the detergent solubilization does not destroy the protein binding capacity.
D000818:Animals; D001665:Binding Sites; D002413:Cations, Divalent; D004492:Edetic Acid / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D066298:In Vitro Techniques; D007700:Kinetics; D008566:Membranes / Q000187:drug effects / Q000378:metabolism; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D011092:Polyethylene Glycols; D011485:Protein Binding; D051381:Rats; D011955:Receptors, Drug; D014532:Uridine Diphosphate Glucose / Q000378:metabolism* / Q000494:pharmacology; D014539:Uridine Diphosphate Sugars / Q000378:metabolism*
D002413:Cations, Divalent; D011955:Receptors, Drug; D014539:Uridine Diphosphate Sugars; D011092:Polyethylene Glycols; D004492:Edetic Acid; D014532:Uridine Diphosphate Glucose
Mise en évidence d'un récepteur d'UDP-glucose dans les membranes microsomiques des hepatocytes de rat