Separation of four components of the phosphoenolpyruvate: glucose phosphotransferase system in Vibrio parahaemolyticus.
Four classes of Vibrio parahaemolyticus mutants defective in the phosphoenolpyruvate: glucose phosphotransferase system (PTS) are described. They were phenotypically different, and were defective in different PTS components. The components designated tentatively as II, I, III, and H were separated by gel filtration of a wild-type extract. Component II, which was specific for glucose and found in the particulate fraction, is probably membrane-bound, glucose-specific enzyme II. Both components I and H were soluble proteins, and the latter was relatively heat-stable. Component I was required for phosphorylation of glucose, trehalose, fructose, mannose, and mannitol. Component H was also required for phosphorylating all the above sugars except fructose. These and some additional findings strongly suggest that components I and H correspond to enzyme I and HPr, respectively. Component III, a soluble heat-stable protein, may be equivalent to the sugar-specific factor III found in other organisms, although it seems to participate in phosphorylating two sugars, glucose and trehalose. There were evidences that mutants defective in components I and III were deficient in cyclic adenosine 3',5'-monophosphate synthesis under certain conditions.
D050260:Carbohydrate Metabolism; D000242:Cyclic AMP / Q000096:biosynthesis; D004926:Escherichia coli / Q000201:enzymology; D005816:Genetic Complementation Test; D005947:Glucose; D009154:Mutation; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000032:analysis* / Q000378:metabolism; D014736:Vibrio parahaemolyticus / Q000201:enzymology* / Q000235:genetics
D000242:Cyclic AMP; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; D005947:Glucose