Nucleotide sequence analysis of two simian virus 40 mutants with deletions in the region coding for the carboxyl terminus of the T antigen.
Nucleotide sequence analysis of two simian virus 40 early mutants dl1263 and dl1265, which lack a DNA segment around map positions 0.21 and 0.18, respectively (C. Cole, T. Landers, S. Goff, S. MAnteuil-Brutlag, and P. Berg, J. Virol. 24:277--294, 1977), revealed in-phase deletions of 33 nucleotide pairs for dl1263 and 39 nucleotide paris for dl1265. The 33-base-pair deletion in dl1263 does not correspond to an apparent shortening by 6,000 daltons observed for the mutant T antigen. In dl1265 the normal termination signal as well as most of the proline-rich terminal tryptic peptide has been removed, and the carboxyl terminus of the mutant T antigen is a series of three cysteine residues.
D000956:Antigens, Viral / Q000235:genetics*; D001483:Base Sequence; D002874:Chromosome Mapping; D004279:DNA, Viral / Q000032:analysis*; D005814:Genes, Viral; D005815:Genetic Code; D009154:Mutation; D009711:Nucleotides / Q000032:analysis*; D013539:Simian virus 40 / Q000032:analysis / Q000235:genetics*
D000956:Antigens, Viral; D004279:DNA, Viral; D009711:Nucleotides