Galactose-6-phosphate dehydrogenase. Purification and partial characterization.
A new enzyme, galactose-6-phosphate dehydrogenase has been purified about 50-fold from goat liver. The enzyme can be distinguished from the nonspecific hexose-6-phosphate dehydrogenase and glucose-6-phosphate dehydrogenase by its high substrate specificity and absolute pyridine nucleotide requirement. In contrast to the hexose-6-phosphate dehydrogenase, this enzyme is located exclusively in the cytoplasmic fraction of the cell. The enzyme is a metalloprotein and is highly sensitive to mercurials. The product of the reaction is possibly a ketoaldose, phosphorylated at the primary alcoholic group.
D000429:Alcohol Oxidoreductases / Q000302:isolation & purification* / Q000378:metabolism; D000818:Animals; D002237:Carbohydrate Dehydrogenases; D002413:Cations, Divalent; D002414:Cations, Monovalent; D002729:Chloromercuribenzoates / Q000494:pharmacology; D002848:Chromatography, DEAE-Cellulose; D003593:Cytoplasm / Q000201:enzymology; D004492:Edetic Acid / Q000494:pharmacology; D005690:Galactose; D006041:Goats; D006600:Hexosephosphates; D006863:Hydrogen-Ion Concentration; D007461:Iodoacetates / Q000494:pharmacology; D007501:Iron / Q000494:pharmacology; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008623:Mercaptoethanol / Q000494:pharmacology; D009243:NAD; D009249:NADP; D011479:Protamines
D002413:Cations, Divalent; D002414:Cations, Monovalent; D002729:Chloromercuribenzoates; D006600:Hexosephosphates; D007461:Iodoacetates; D011479:Protamines; D009243:NAD; D009249:NADP; D008623:Mercaptoethanol; D004492:Edetic Acid; D007501:Iron; D000429:Alcohol Oxidoreductases; D002237:Carbohydrate Dehydrogenases; C020552:galactose-6-phosphate dehydrogenase; D005690:Galactose