Glycopeptides of murine leukemia viruses. I. Comparison of two ecotropic viruses.
The glycopeptides obtained by pronase digestion of two ecotropic strains of murine leukemia virus (MuLV) were compared by gel filtration. Four different glycopeptide size classes, designated G(1), G(2), G(3), and G(4), with molecular weights of approximately 5,100, 2,900, 2,200, and 1,500, respectively, were shown to be associated with Rauscher MuLV virions grown in JLS-V9 cells. Various sugar precursors, including glucosamine, galactose, fucose, and mannose were incorporated into G(1) and G(2), suggesting that these are complex (type I) glycopeptides. The two smaller glycopeptide size classes, G(3) and G(4), were shown to be mannoserich (type II) glycopeptides. G(4) was more sensitive to digestion with endo-beta-N-acetylglucosaminidase H than G(3), suggesting that the core of G(3) may contain fewer mannose residues. Glycopeptides of the same size class as G(1) and G(2) were associated with both Rauscher MuLV and AKR-MuLV grown in III6A (mouse embryo) cells. Previous studies have shown that gp52, a proteolytic cleavage product of gp70, possessed primarily G(1) glycopeptides and that gp52 was more highly sulfated than gp70. We observed that G(1) is approximately twofold more highly sulfated than G(2), explaining the observed difference in sulfation of gp52. The unusually large size of G(1) suggested that infection with MuLV may alter the host cell glycosylation pattern. To test this possibility, glycopeptides from Sindbis virions grown in uninfected and Rauscher MuLV-infected JLS-V9 cells were compared, and no differences were observed. G(1) was not detected in Sindbis virions, indicating that acquisition of G(1) depends on properties of the virus-coded polypeptide backbone of the gp70 molecule.
D000406:AKR murine leukemia virus / Q000032:analysis*; D000818:Animals; D002460:Cell Line; D006020:Glycopeptides / Q000032:analysis*; D006023:Glycoproteins / Q000032:analysis; D009052:Leukemia Virus, Murine / Q000032:analysis*; D007942:Leukemia, Experimental / Q000382:microbiology; D051379:Mice; D008970:Molecular Weight; D009844:Oligosaccharides / Q000032:analysis; D011925:Rauscher Virus / Q000032:analysis* / Q000254:growth & development; D012845:Sindbis Virus / Q000032:analysis / Q000254:growth & development; D014764:Viral Proteins / Q000032:analysis
D006020:Glycopeptides; D006023:Glycoproteins; D009844:Oligosaccharides; D014764:Viral Proteins