title: Nationwide epidemic of septicemia caused by contaminated infusion products. IV. Growth of microbial pathogens in fluids for intravenous infusions.
abstract: Septicemia caused by contaminated infusion fluid is a newly appreciated hazard of intravenous infusion therapy. Microorganisms of the tribe Klebsielleae (Klebsiella, Enterobacter, and Serratia) have predominated in these infections. Members of this tribe found to possess a selecive ability over common non-Klebsielleae microbial pathogens to proliferate rapidly in commerical parential fluids contaning clucose at room temperautre. Fifty-one Klebsielleae strains, washed twice before inoculation of approximately 1 organism/ml, attained a mean normalized 24 hr concentration of 1.11 x 10-5 organisms/ml in 5% dextrose in water at 25 C. In contrast, 48 of 49 non-Klebsielleae bacterial strains (clinical isolates of Staphylococcus, Proteus, Escherichia coli, Herelea, and Pseudomonas aeruginosa) slowly died (mean 24-hr concentration, 0.2 organism/ml). Five Candida albicans strains frew only very slowly (31.3 organisms/ml). Even with concentrations exceeding 10-6 organisms/ml, microbial presence was never visibly detectable. The significant increases in cases of nosocomial spticmia caused by Klebsiella, Enterobacter, and Serratia in recent years might be attribuatble in part to fluid-related spesis accompanying the expanding use of parenteral therapy.
mesh_terms: D001419:Bacteria / Q000254:growth & development; D002176:Candida albicans / Q000254:growth & development / Q000302:isolation & purification; D004196:Disease Outbreaks / Q000453:epidemiology*; D004340:Drug Contamination; D004345:Drug Industry / Q000592:standards; D004755:Enterobacteriaceae / Q000302:isolation & purification; D005947:Glucose; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007263:Infusions, Parenteral / Q000009:adverse effects*; D007709:Klebsiella / Q000302:isolation & purification; D007710:Klebsiella Infections; D018805:Sepsis / Q000209:etiology* / Q000382:microbiology; D012705:Serratia / Q000302:isolation & purification; D012965:Sodium Chloride; D014481:United States; D014867:Water
chemical_list: D014867:Water; D012965:Sodium Chloride; D005947:Glucose