Studies on antiviral glycosides. II. Mode of action for virucidal effects on Sendai virus.
Treatment of Sendai virus with p-(sec-butyl)-phenyl-6-chloro-6-deoxy-beta-D-glucopyranoside, followed by freezing and thawing resulted in a loss of hemolytic and cell fusion activities as well as infectivity without affecting hemagglutinating and neuraminidase activities. The anti-hemolytic activity of this compound was reversed by the addition of phosphatidyl choline to the virus samples. p-Azidophenyl-6-chloro-6-deoxy-beta-D[3H]glucopyranoside was successfully used for photoaffinity labeling of a specific virion site, and we confirmed the affected site of the glucoside to be the lipid components in the viral envelopes.
D000998:Antiviral Agents; D002459:Cell Fusion; D005615:Freezing; D005960:Glucosides / Q000494:pharmacology*; D006027:Glycosides / Q000494:pharmacology*; D006384:Hemagglutination; D006461:Hemolysis; D009439:Neuraminidase / Q000378:metabolism; D010222:Parainfluenza Virus 1, Human / Q000187:drug effects* / Q000502:physiology
D000998:Antiviral Agents; D005960:Glucosides; D006027:Glycosides; C020240:p-(sec-butyl)-phenyl-6-chloro-6-deoxy-beta-D-glucopyranoside; D009439:Neuraminidase