Quantitative measurement of adenosine deaminase from human erythrocytes.
Methods for measuring enzymatic activity of adenosine deaminase from human erythrocytes were examined and compared with each other. Determination of ADA by the method in which adenosine is converted into inosine with uric acid as the final product by the action of nucleoside phosphorylase and xanthine oxidase appears to yield the most reliable results. In the recommended assay saponin is used for lysis of erythrocytes when testing adenosine deaminase activity in red blood cells. Storage of erythrocyte samples is optimal at +4 degrees C; storage at room temperature or at -20 degrees C leads to loss of adenosine deaminase activity.
D000243:Adenosine Deaminase / Q000097:blood*; D004355:Drug Stability; D004912:Erythrocytes / Q000201:enzymology*; D005069:Evaluation Studies as Topic; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008722:Methods; D009700:Nucleoside Deaminases / Q000097:blood*; D013696:Temperature; D013997:Time Factors
D009700:Nucleoside Deaminases; D000243:Adenosine Deaminase