Labilization of lysosomes and mitochondria in situ by hypoxia and hypoxia-related factors.
Primary cultures of rat heart muscle and endothelioid cells were used to study the labilization of lysosomes and mitochondria by hypoxia, "hypoxia substitutes", and the in vivo-related factors of free fatty acid and pH. Subliminally-active levels tested were: hypoxia (1% O2/4 days), 5x10(-6)M sodium stearate: albumin in 6:1 ratio/30 min, KCN (1x10(-3)M/1 hr), 2 deoxy-glucose (3x10(-2)M/12 hr), acidosis (pH 6.9/30 min) and combinations. KCN plus 2-DG labilized the organelles in both cell types. Sensitivity to injury was in the order mitochondria greater than lysosomes and muscle cells greater than endothelioid cells.
D000818:Animals; D002478:Cells, Cultured; D003486:Cyanides / Q000494:pharmacology; D003847:Deoxyglucose / Q000494:pharmacology; D004727:Endothelium / Q000648:ultrastructure; D006863:Hydrogen-Ion Concentration; D000860:Hypoxia / Q000473:pathology / Q000503:physiopathology*; D008247:Lysosomes / Q000473:pathology*; D008931:Mitochondria, Muscle; D009206:Myocardium / Q000648:ultrastructure; D010539:Permeability; D051381:Rats; D013229:Stearic Acids / Q000494:pharmacology
D003486:Cyanides; D013229:Stearic Acids; D003847:Deoxyglucose