DEAE-silica gel and DEAE-controlled porous glass as ion exchangers for isolation of glycolipids.
DEAE-silica gel and DEAE-controlled porous glass have been used for the quantitative isolation of gangliosides and neutral glycosphingolipids from animal tissues and cells. A direct comparative study between DEAE-silica gel, DEAE-controlled porous glass and DEAE-Sephadex was made; the results indicated that DEAE-silica gel is preferable to the other two ion exchangers. DEAE-silica gel has also been found to be suitable for the fractionation of ganglioside mixtures.
D000328:Adult; D000818:Animals; D001923:Brain Chemistry; D002417:Cattle; D002852:Chromatography, Ion Exchange; D004912:Erythrocytes / Q000032:analysis; D005732:Gangliosides / Q000302:isolation & purification; D005782:Gels; D005898:Glass; D006028:Glycosphingolipids / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D012822:Silicon Dioxide
D005732:Gangliosides; D005782:Gels; D006028:Glycosphingolipids; D012822:Silicon Dioxide