Isolation and properties of poly(A)-containing RNA from the cytoplasm of rat liver cells.
1. The described technique for preparative isolation of poly(A)-containing RNA by complexing with poly(U)-cellulose and elution at 45 degrees C, is simple, reproducible, and the poly(U)-cellulose preparation is suitable for repeated use. 2. The poly(A)-containing RNA isolated from the poly(U)-cellulose complex is still contaminated by ribosomal RNA which can be removed by recomplexing. The purified preparation is heterogeneous on polyacrylamide-gel electrophoresis and is located over the region from 5-6S to 28S. Poly(A) segments of cytoplasmic RNA show heterogeneous size distribution. 3. In acidic medium, the poly(A) segments form ordered double-stranded structure. 4. The rapid labelling of rat liver and fibroblast cytoplasmic poly(A)-containing RNAs and their base composition resemble closely the corresponding properties of mRNA.
D000227:Adenine Nucleotides / Q000032:analysis*; D000241:Adenosine / Q000378:metabolism; D000818:Animals; D002250:Carbon Radioisotopes; D002478:Cells, Cultured; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D003593:Cytoplasm / Q000032:analysis*; D003609:Dactinomycin / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000648:ultrastructure; D008722:Methods; D009691:Nucleic Acid Denaturation; D009711:Nucleotides / Q000032:analysis; D009963:Orotic Acid / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D010761:Phosphorus Radioisotopes; D011063:Poly A-U; D011119:Polynucleotides / Q000032:analysis*; D012313:RNA / Q000302:isolation & purification*; D051381:Rats; D013696:Temperature; D014316:Tritium; D014462:Ultrafiltration; D014529:Uridine / Q000378:metabolism
D000227:Adenine Nucleotides; D002250:Carbon Radioisotopes; D009711:Nucleotides; D010710:Phosphates; D010761:Phosphorus Radioisotopes; D011119:Polynucleotides; D014316:Tritium; D003609:Dactinomycin; D011063:Poly A-U; D009963:Orotic Acid; D012313:RNA; D000241:Adenosine; D014529:Uridine