title: Presence of viral particles in the salivary gland of Calomys musculinus infected with Junin virus by a natural route.
abstract: Calomys musculinus, a wild cricetid rodent, is one of the main reservoirs of Junin virus. Six of these animals were infected by being placed in close contact with animals that had been experimentally infected with the virus. They were sacrificed at 10, 15 and 20 months after contact, and their salivary glands were studied by ultrastructural, immunohistochemical and virological methods. Two animals developed chronic viremia and low titers of complement-fixing antibodies. These animals were the only ones that had high viral titers in salivary glands and blood and viral antigen and particles in salivary glands. Although some of the other animals had viremia at the beginning of the experiment, it was absent 5 months later. Complement-fixing antibodies developed in all animals. On the basis of these findings, we assumed that the salivary gland is an important site of viral synthesis and excretion. This type of chronic infection, with persistent viremia and virus shedding, is possibly important for virus perpetuation in nature and transmission to man.
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D001116:Arenaviridae / Q000254:growth & development*; D006477:Arenaviruses, New World / Q000254:growth & development* / Q000276:immunology / Q000302:isolation & purification; D001769:Blood / Q000382:microbiology; D004197:Disease Reservoirs; D006478:Hemorrhagic Fever, American / Q000382:microbiology*; D012377:Rodentia; D012469:Salivary Glands / Q000276:immunology / Q000382:microbiology*
chemical_list: D000956:Antigens, Viral