Cyclization of uridine monophosphate by diethyl pyrocarbonate.
Reaction between diethyl pyrocarbonate and uridine 2'-phosphate or uridine 3'-phosphate leads to the formation in high yields of uridine 2':3'-cyclic phosphate. This reaction product was identified in experiments involving (a) ultraviolet spectrophotometry, (b) paper chromatography, (c) high voltage paper electrophoresis at both  pH 3.5 and 7.4, (d) acid hydrolysis, and (e) digestion with pancreatic ribonuclease.
D000469:Alkaline Phosphatase; D002854:Chromatography, Paper; D004047:Diethyl Pyrocarbonate; D004590:Electrophoresis, Paper; D005561:Formates; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D009712:Nucleotides, Cyclic / Q000138:chemical synthesis*; D012260:Ribonucleases; D013056:Spectrophotometry, Ultraviolet; D014500:Uracil Nucleotides
D005561:Formates; D009712:Nucleotides, Cyclic; D014500:Uracil Nucleotides; D012260:Ribonucleases; D000469:Alkaline Phosphatase; D004047:Diethyl Pyrocarbonate