title: Kinetic properties of an enzyme highly enriched in carbon-13.
abstract: Preparations of Candida utilis 6-phosphogluconate dehydrogenase with extreme variation in carbon isotope composition have been obtained. Molecular activities, pH optima and substrate affinities of these enzyme preparations have been measured. It was observed that any effect of isotope composition on these physiologically important properties is minimal.
mesh_terms: D000085:Acetates / Q000378:metabolism; D002175:Candida / Q000201:enzymology; D002247:Carbon Isotopes; D003470:Culture Media; D005942:Gluconates; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009249:NADP; D010734:Phosphogluconate Dehydrogenase / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D000085:Acetates; D002247:Carbon Isotopes; D003470:Culture Media; D005942:Gluconates; D009249:NADP; D010734:Phosphogluconate Dehydrogenase