The effects of carbodiimides on functions associated with the energy-conservation mechanism in beef heart sub-mitochondrial particles.
N,N'-di-n-propyl-, N,N'di-n-butyl-, N,N'-di-n-pentyl-, N,N'di-n-hexyl-, N,N'di-n-octoyl, N,N'-dibenzhydryl-, and N,N'-dibenzhydrylcarbodiimides were synthesized. They were all effective inhibitors (2 nmoles carbodiimide per milligram protein) of the ATP-driven reduction of NAD by succinate and the ATP-driven transhydrogenase activities catalyzed by beef heart submitochondrial particles (SMP). They had no effect on the nonenergy-linked transhydrogenase and stimulated the succinate-driven aerobic transhydrogenase activity of beef heart SMP. It was concluded that they exert their effects by reacting with the N,N'-dicyclohexylcarbodiimide-binding protein. Water-soluble carbodiimides were not effective inhibitors.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002234:Carbodiimides / Q000494:pharmacology*; D002417:Cattle; D004734:Energy Metabolism / Q000187:drug effects*; D007700:Kinetics; D008929:Mitochondria, Heart / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D009250:NADP Transhydrogenases / Q000378:metabolism; D009840:Oligomycins / Q000494:pharmacology; D010085:Oxidative Phosphorylation / Q000187:drug effects; D013367:Submitochondrial Particles / Q000378:metabolism; D013386:Succinates / Q000494:pharmacology
D002234:Carbodiimides; D009840:Oligomycins; D013386:Succinates; C031004:oligomycin A; D009243:NAD; D000255:Adenosine Triphosphate; D009250:NADP Transhydrogenases