Synthesis of sugar-modified nucleoside 5'-triphosphates with partially purified nucleotide kinases from calf thymus.
The partial purification of some nucleoside monophosphate kinases (ATP:nucleosidemonophosphate phosphotransferases, EC 2.7.4.4) from calf thymus by chromatography on Blue Sepharose to remove interfering phosphatase activity is described. Their specificities towards nucleoside monophosphates modified in the sugar are investigated. Pyrimidine nucleoside monophosphate kinase is not very much affected by such modifications, whereas GMP kinase does not tolerate such alteration. The effect on AMP kinase is intermediate.
D000818:Animals; D002417:Cattle; D002850:Chromatography, Gel; D009703:Nucleoside-Phosphate Kinase / Q000302:isolation & purification*; D009711:Nucleotides / Q000138:chemical synthesis* / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D010770:Phosphotransferases / Q000302:isolation & purification*; D013950:Thymus Gland / Q000201:enzymology*
D009711:Nucleotides; D010770:Phosphotransferases; D009703:Nucleoside-Phosphate Kinase; D010744:Phosphoric Monoester Hydrolases