Mucosaccharide histochemistry and histoenzymorphologic observations on the epidermis of Ariosoma balearicum de la Roche (Anguilliformes, Pisces).
The epidermis of Ariosoma balearicum consists of three layers - the basal layer, the middle layer and the outer layer. In between the basal cells are found clusters of small lymphocytes which show a moderat acid phosphatase activity. The middle layer and the outer layer are composed of three types of cells - the polygonal cells, the mucous cells and the club cells. The mucosubstances within the mucous cells exhibit the properties of neuraminic acid containing mucosaccharides with vicinal hydroxyl, sulfate esters and carboxyl groupings. The superficial cells of the outermost layer are capable of secreting sulphated and carboxylated mucosubstances to form an extracellular mucous coating which in fish epidermis seems to be necessary for the accumulation of electrolytes. The club cells are generally provided with one nucleus or double nuclei which appear displaced from the center and constitute the main histological component of the middle layer. The contents of these cells, in addition to a strong protein uptake visualized by the positive reactions exhibited in the coupled tetrazonium and mercuric bromophenol blue preparations, gave positive response to neutral mucosubstances which appear to be a glycoprotein involved in the slime secretions. Alkaline phosphatase and TPP-hydrolysing enzyme which could be detectable in the basal layer and the basement membrane were correlated with the probable role that they could play in the transportation of various chemicals and nutriments through the cell membranes.
D000135:Acid Phosphatase / Q000032:analysis; D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D000078404:Epidermal Cells; D004817:Epidermis / Q000032:analysis*; D005260:Female; D005399:Fishes / Q000033:anatomy & histology / Q000378:metabolism*; D006025:Glycosaminoglycans / Q000032:analysis*; D006651:Histocytochemistry; D008297:Male; D009077:Mucins / Q000032:analysis*; D010710:Phosphates / Q000032:analysis*; D011755:Pyrophosphatases / Q000032:analysis*; D013834:Thiamine Pyrophosphatase / Q000032:analysis*
D006025:Glycosaminoglycans; D009077:Mucins; D010710:Phosphates; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D011755:Pyrophosphatases; D013834:Thiamine Pyrophosphatase