Cerebral cortical microfluorometry at isosbestic wavelengths for correction of vascular artifact.
Microfluorometric measurements of cerebral cortical mitochondrial respiration in vivo are obscured by hemodynamic and oximetric artifacts. Isosbestic fluorometry provides appropriate correction for these vascular phenomena and permits simultaneous evaluation of mitochondrial nicotinamide adenine dinucleotide redox state, microcirculatory volume, and hemoglobin oxygenation.
D000818:Animals; D001923:Brain Chemistry; D002540:Cerebral Cortex / Q000032:analysis*; D004285:Dogs; D005470:Fluorometry / Q000295:instrumentation / Q000379:methods*; D006454:Hemoglobins / Q000032:analysis; D002534:Hypoxia, Brain / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D009243:NAD / Q000032:analysis* / Q000378:metabolism; D010084:Oxidation-Reduction; D010100:Oxygen / Q000097:blood; D012235:Rhodamines
D006454:Hemoglobins; D012235:Rhodamines; D009243:NAD; D010100:Oxygen