Local versus regional procurement and distribution of granulocytes.
Granulocyte concentrates obtained by discontinuous flow centrifugation (DFC) and continuous flow centrifugation (CFC) were studied. The DFC granulocytes were obtained from a regional center and stored for 24 hours prior to transfusion. The CFC granulocytes were obtained locally and transfused within a few hours. Even at 24 hours, DFC granulocytes had significantly reduced bactericidal capacity, chemiluminescence, nitroblue tetrazolium reduction, chemotaxis and random mobility. Granulocyte kinetics utilizing DF32P and skin windows demonstrated the ability of DFC granulocytes to circulate and migrate into the tissues despite the in vitro abnormalities. Until effective storage techniques for granulocyte preservation became available, rapid transportation and processing from regional centers or local procurement of granulocytes is necessary to transfuse functional granulocytes.
D001770:Blood Bactericidal Activity; D001782:Blood Donors; D001793:Blood Preservation; D002465:Cell Movement; D002470:Cell Survival; D002498:Centrifugation; D002634:Chemotaxis, Leukocyte; D006098:Granulocytes / Q000473:pathology; D006801:Humans; D007700:Kinetics; D007958:Leukocyte Count; D008163:Luminescent Measurements; D009580:Nitroblue Tetrazolium; D009195:Peroxidase; D010586:Phagocytes
D009580:Nitroblue Tetrazolium; D009195:Peroxidase