Cell-free synthesis and processing of the proteins of poliovirus.
Poliovirus RNA can be translated completely and accurately in rabbit reticulocyte lysates; the nascent poly-protein is processed to give primary products 1a, X, and 1b indistinguishable from those made in poliovirus-infected HeLa cells. The capsid precursor protein 1a is processed to form the capsid proteins VP0, VP1, and VP3, while the noncapsid precursor 1b is processed to form protein 2.
D000818:Animals; D002474:Cell-Free System; D007700:Kinetics; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis; D010447:Peptide Hydrolases / Q000378:metabolism; D017955:Poliovirus / Q000378:metabolism*; D011498:Protein Precursors / Q000096:biosynthesis*; D011817:Rabbits; D012156:Reticulocytes; D014764:Viral Proteins / Q000096:biosynthesis*
D010446:Peptide Fragments; D011498:Protein Precursors; D014764:Viral Proteins; D010447:Peptide Hydrolases