[Fragments of linear unintegrated Rous sarcoma virus DNA, resulting from digestion with SmaI restriction endonuclease].
Linear unintegrated DNA of Schmidt-Ruppin Rous sarcoma virus, subgroup D (SR-RSV-D), was digested with SmaI restriction endonuclease, analyzed by agarose gel electrophoresis, "blotted" by Southern's method and hybridized with a viral 32P cDNA. SmaI cleaves this DNA at five sites, two of which are localized at the ends of the provirus. Src and env genes seem not to be restrictied by SmaI cleavage.
D000818:Animals; D001358:Avian Sarcoma Viruses / Q000032:analysis*; D002642:Chick Embryo; D004262:DNA Restriction Enzymes; D004279:DNA, Viral; D015252:Deoxyribonucleases, Type II Site-Specific; D005347:Fibroblasts; D005814:Genes, Viral; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization
D004279:DNA, Viral; D004262:DNA Restriction Enzymes; C025651:CCCGGG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific
Fragments de l'acide désoxyribonucléique linéaire du virus du sarcome de Rous digéré par l'endonucléase de restriction SmaI.