Characterization of pyrimidine-repressible and arginine-repressible carbamyl phosphate synthetases from Bacillus subtilis.
The number and properties of carbamyl phosphate synthetases in Bacillus subtilis have been uncertain because of conflicting genetic results and instability of the enzyme in extracts. The discovery of a previously unrecognized requirement of B. subtilis carbamyl phosphate synthetases for a high concentration of potassium ions for activity and stability permitted unequivocal demonstration that this bacterium elaborates two carbamyl phosphate synthetases. Carbamyl phosphate synthetase A was shown to be repressed by arginine, to have a molecular weight of about 200,000, and to be coded for by a gene that maps near argC4. This isozyme was insensitive to metabolites of the arginine and pyrimidine biosynthetic pathways. Carbamyl phosphate synthetase P was found to be repressed by uracil, to have a molecular weight of 90,000 to 100,000, and to be coded for by a gene that maps near the other pyr genes. This isozyme was activated by phosphoridine nucleotides. Other kinetic properties of the two isozymes were compared. Bacillus thus resembles eucaryotic microbes in producing two carbamyl phosphate synthetases, rather than the enteric bacteria, which produce a single carbamyl phosphate synthetase.
D001120:Arginine / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000201:enzymology*; D002223:Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing) / Q000378:metabolism*; D004794:Enzyme Repression; D006150:Guanine Nucleotides / Q000494:pharmacology; D006358:Hot Temperature; D007527:Isoenzymes / Q000378:metabolism*; D007700:Kinetics; D008970:Molecular Weight; D010754:Phosphoribosyl Pyrophosphate / Q000494:pharmacology; D010770:Phosphotransferases / Q000378:metabolism*; D014498:Uracil / Q000494:pharmacology*; D014500:Uracil Nucleotides / Q000494:pharmacology
D006150:Guanine Nucleotides; D007527:Isoenzymes; D014500:Uracil Nucleotides; D014498:Uracil; D010754:Phosphoribosyl Pyrophosphate; D001120:Arginine; D010770:Phosphotransferases; D002223:Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)