[Isolation of DNA fraction bound to the axial structure of metaphase chromosomes and studies of renaturation].
Mitotic chromosomes of L cells (metaphase plates) were dehistonized by centrifugation through a layer of 2 M NaCl and then treated with restriction endonuclease EcoRI and HindIII. Alternatively, they were pretreated with EcoRI endonuclease. The DNA remaining attached to the axial structure of the chromosomes was isolated and investigated in renaturation experiments. It was found to be enriched in reiterated base sequences belonging to the satellite and to abundant intermediate repeats.
D000818:Animals; D001483:Base Sequence; D002453:Cell Cycle; D002875:Chromosomes / Q000032:analysis*; D004247:DNA / Q000302:isolation & purification; D004262:DNA Restriction Enzymes; D007700:Kinetics; D007739:L Cells / Q000166:cytology; D008677:Metaphase; D051379:Mice; D009695:Nucleic Acid Renaturation
D004247:DNA; D004262:DNA Restriction Enzymes
Vydelenie traktsii DNK, Prilegaiushcheĭ k ostovy metafaznoĭ khromosomy, i izuchenie ee renaturatsionnykh svoĭstv.