Hemoglobin Cochin-Port-Royal: consequences of the replacement of the beta chain C-terminal by an arginine.
Hemoglobin Cochin Port-Royal beta 146 (HC3) His yields Arg is the second example in which the beta C-terminal residue is replaced. Owing to the known importance of His beta 146 in the co-operative effects of hemoglobin, the functional properties of this variant were carefully studied. It had a normal Hill coefficient but a reduced alkaline Bohr effect. However, the reduction in Bohr effect is less than the halving predicted from previous mutants and modified hemoglobins.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D001120:Arginine / Q000032:analysis; D004163:Diphosphoglyceric Acids / Q000097:blood; D004592:Electrophoresis, Starch Gel; D005602:France; D006455:Hemoglobins, Abnormal / Q000032:analysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D010100:Oxygen / Q000097:blood; D010446:Peptide Fragments / Q000032:analysis
D000596:Amino Acids; D004163:Diphosphoglyceric Acids; D006455:Hemoglobins, Abnormal; D010446:Peptide Fragments; D001120:Arginine; D010100:Oxygen