A novel method for the isolation and study of a magnetotactic bacterium.
The magnetococcus, a magnetotactic bacterium, has been grown in a complex simulated natural environment. Sufficiently pure samples of cells were obtained magnetically making axenic cultures unnecessary for many purposes. The magnetococcus is a Gram-negative coccus, 1.6 micron in diameter and readily distinguished by highly refractile inclusions and its magnetotactic behavior. This organism is actively motile by means of two bundles of flagella. Electron dense ferromagnetic inclusions were localized between the flagellar bundles. Collections of magnetococci were morphologically homogeneous and negligibly contaminated by extraneous bacteria. DNA extracted from pooled collections of cells was homogeneous by analytical CsC1 centrifugation. The guanine-cytosine content was 61.7%. Total iron by percent cellular dry weight was 3.8%. Comparisons with a previously described magnetotactic marine coccus were made.
D001419:Bacteria / Q000302:isolation & purification* / Q000648:ultrastructure; D018407:Bacterial Physiological Phenomena; D001431:Bacteriological Techniques; D004269:DNA, Bacterial / Q000032:analysis; D005407:Flagella / Q000648:ultrastructure; D002479:Inclusion Bodies / Q000648:ultrastructure; D007501:Iron / Q000032:analysis; D008280:Magnetics; D009068:Movement; D012722:Sewage; D014871:Water Microbiology
D004269:DNA, Bacterial; D012722:Sewage; D007501:Iron