Utilization of endogenous diacylglycerol for the synthesis of triacylglycerol, phosphatidylcholine and phosphatidylethanolamine by lipid particles from baker's yeast (Saccharomyces cerevisiae).
The activity of the enzymes diacylglycerol acyltransferase (EC 2.3.1.20), cholinephosphotransferase (EC 2.7.8.2) and ethanolaminephosphotransferase (EC 2.7.8.1) have been measured in a lipid particle preparation from baker's yeast (Saccharomyces cerevisiae) with endogenous 1,2-diacylglycerol as substrate. For all three enzymes the rate of diacylglycerol utilization was established with respect to substrate and Mg2+ concentration. Neither of the enzyme activities was stimulated significantly by addition of diacylglycerols. The conversion of diacylglycerol into triacylglycerol in the presence of CDP-choline and CDPethanolamine, and the synthesis of phospholipids in the presence of acyl-CoA either added or generated in situ were studied. Neither CDPcholine nor CDPethanolamine had an effect on triacylglycerol synthesis. Exogenous acyl-CoA had no effect on either choline- or ethanolaminephosphotransferase activity. However, when the necessary substrates for formation of acyl-CoAs in situ (ATP, CoA, Mg2+ and free fatty acids) were added a decrease in both cholinephosphotransferase and ethanolaminephosphotransferase activity was observed. This inhibition was shown to be due to ATP and might explained as a result of chelation of the Mg2+, a necessary activator of both the choline- and the ethanolaminephosphotransferase.
D000105:Acetyl Coenzyme A / Q000494:pharmacology; D000217:Acyltransferases / Q000378:metabolism; D003565:Cytidine Diphosphate / Q000031:analogs & derivatives / Q000494:pharmacology; D003566:Cytidine Diphosphate Choline / Q000494:pharmacology; D002798:Diacylglycerol Cholinephosphotransferase / Q000378:metabolism; D004075:Diglycerides / Q000378:metabolism*; D004982:Ethanolaminephosphotransferase / Q000378:metabolism; D004983:Ethanolamines / Q000494:pharmacology; D005989:Glycerides / Q000378:metabolism*; D008861:Microsomes / Q000378:metabolism*; D010713:Phosphatidylcholines / Q000096:biosynthesis*; D010714:Phosphatidylethanolamines / Q000096:biosynthesis*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D014280:Triglycerides / Q000096:biosynthesis*
D004075:Diglycerides; D004983:Ethanolamines; D005989:Glycerides; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D014280:Triglycerides; D003566:Cytidine Diphosphate Choline; D003565:Cytidine Diphosphate; D000105:Acetyl Coenzyme A; D000217:Acyltransferases; D004982:Ethanolaminephosphotransferase; D002798:Diacylglycerol Cholinephosphotransferase