[Role of tropomyosin in the sensitivity of (Na+ + K+) ATPase to ouabain].
EDTA treatment of isolated plasma membranes from MF2S cells increased 1,000 fold the sensitivity of (Na+ + K+) ATPase activity to ouabain. The original sensitivity of the enzyme to the drug is recovered after addition of tropomyosin together with Ca++ ions to the treated membranes.
D000818:Animals; D001665:Binding Sites / Q000187:drug effects; D002462:Cell Membrane / Q000201:enzymology; D004492:Edetic Acid / Q000494:pharmacology; D066298:In Vitro Techniques; D008565:Membrane Proteins / Q000502:physiology; D051379:Mice; D010042:Ouabain / Q000494:pharmacology*; D011817:Rabbits; D000254:Sodium-Potassium-Exchanging ATPase / Q000037:antagonists & inhibitors*; D014335:Tropomyosin / Q000494:pharmacology / Q000502:physiology*
D008565:Membrane Proteins; D014335:Tropomyosin; D010042:Ouabain; D004492:Edetic Acid; D000254:Sodium-Potassium-Exchanging ATPase
Rôle de la tropomyosine dans la sensibilité de l'ATPase (Na+ + K+) à l'ouabaïne.