title: Comparative study of ten bacteriocins of Clostridium perfringens.
abstract: Bacteriocins of Clostridium perfringens were prepared by ammonium sulfate precipitation of supernatant broth from 10 bacteriocinogenic strains. These bacteriocins were compared with respect to their ability to produce spheroplasts in a sensitive indicator strain; their inducibility; sensitivity to pH, proteolytic enzymes, and boiling; and their effect on macromolecular synthesis. Two bacteriocins were stable over a wide range of pH values and resisted boiling, and three bacteriocins were resistant to trypsin. Five bacteriocins shut down DNA, RNA, and protein synthesis; three bacteriocins had varying effects on DNA and RNA synthesis; and two bacteriocins had little effect on macromolecular synthesis. Antiserum prepared against one bacteriocin highly neutralized three bacteriocins with partial neutralization of five others; two bacteriocins were unaffected. Mutant strains selected for resistance to bacteriocin 28 also demonstrated coresistance to two other closely related bacteriocins and partial resistance to five others.
mesh_terms: D001430:Bacteriocins / Q000032:analysis / Q000494:pharmacology*; D003016:Clostridium perfringens / Q000254:growth & development*; D004247:DNA / Q000096:biosynthesis; D012313:RNA / Q000096:biosynthesis; D013104:Spheroplasts / Q000187:drug effects*; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D001430:Bacteriocins; D012313:RNA; D004247:DNA