[Interaction of steroids with human serum albumin by spin echo technique and paramagnetic sound methods].
The rate of water proton relaxation of the solutions of human serum albumin (HSA) modified by different spin labels has been investigated by spin echo technique. The rate of proton relaxation in the presence of the labeled HSA is higher than that in water solutions of spin labels. The proton relaxation data of the protein solutions can be explained by local properties of the water, bound with the protein (in the regions of spin labels) which seem to resemble those of water-glycerol solutions. The rate of proton relaxation was decreased in the presence of steroids that can be explained by a decrease of the local microviscosity of the water-protein matrix due to interaction of steroids with HSA.
D004578:Electron Spin Resonance Spectroscopy; D006801:Humans; D007700:Kinetics; D008433:Mathematics; D011485:Protein Binding; D011487:Protein Conformation; D012709:Serum Albumin; D012996:Solutions; D013113:Spin Labels; D013256:Steroids; D014867:Water
D012709:Serum Albumin; D012996:Solutions; D013113:Spin Labels; D013256:Steroids; D014867:Water
Issledovanie vzaimodeĭstviia steroidov s syvorotochnym al'buminom cheloveka metodom spinovoe ekho-paramagnitnyĭ zond.