title: Electric and flow birefringence properties of myosin in aqueous urea and sodium pyrophosphate.
abstract: The electric dipole moment of rabbit skeletal myosin was estimated from the electric and flow birefringence properties. Myosin formed small polydisperse aggregates (0.2-1.1 microM in length) with an apparent electric dipole moment of 5,000-20,000 Debye in aqueous urea or sodium pyrophosphate at low ionic strength. Permanent dipole moment contributed substantially to the apparent dipole moment. An anti-parallel association of myosin was suggested from the dependence of the apparent dipole moment on myosin concentration. Some interactions between myosin and C-protein were detected in 1 M urea by flow birefringence and analytical ultracentrifugation studies. The apparent dipole moment of myosin aggregates was less dependent on myosin concentration in the presence of C-protein.
mesh_terms: D000818:Animals; D001718:Birefringence; D011756:Diphosphates; D004563:Electrochemistry; D009132:Muscles / Q000032:analysis; D009218:Myosins; D011487:Protein Conformation; D011817:Rabbits; D012995:Solubility; D014508:Urea
chemical_list: D011756:Diphosphates; D014508:Urea; D009218:Myosins