Membrane-mediated killing of Saccharomyces cerevisiae by glycoproteins from Torulopsis glabrata.
Cell-free supernatants from cultures of Torulopsis glabrata contained glycoprotein toxins that killed sensitive and killer strains of Saccharomyces cerevisiae with single-hit kinetics. Growing S. cerevisiae treated with the toxins showed a leakage of cellular potassium, partial dissipation of the adenosine triphosphate pool, and a coordinate shutdown of macromolecular synthesis. These pool efflux-stimulating toxins have been partially purified and at least three toxic glycoproteins have been separated. Pool efflux-stimulating toxin activity was stable from pH 3 through 7, though killing was maximal close to pH 4.
D000255:Adenosine Triphosphate / Q000378:metabolism; D002175:Candida / Q000032:analysis; D002845:Chromatography; D002850:Chromatography, Gel; D004352:Drug Resistance, Microbial; D005656:Fungal Proteins / Q000096:biosynthesis; D006023:Glycoproteins / Q000302:isolation & purification / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D006882:Hydroxyapatites; D009183:Mycotoxins / Q000302:isolation & purification / Q000494:pharmacology*; D009696:Nucleic Acids / Q000096:biosynthesis; D011188:Potassium / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000378:metabolism
D005656:Fungal Proteins; D006023:Glycoproteins; D006882:Hydroxyapatites; D009183:Mycotoxins; D009696:Nucleic Acids; D000255:Adenosine Triphosphate; D011188:Potassium