Effect of boseimycin on some enzyme systems of Bacillus subtilis.
The effect of boseimycin on the in vitro activity and in vivo synthesis of alkaline phosphatase, aconitase and lactate, isocitrate, glutamate and alanine dehydrogenases was studied in Bacillus subtilis. At a subinhibitory concentration, synthesis of glutamate dehydrogenase was stimulated but alkaline phosphatase, lactate dehydrogenase and aconitase synthesis was inhibited. On the contrary, boseimycin inhibited slightly the activity of lactate dehydrogenase in cell-free extracts. Glutamate dehydrogenase and aconitase activities were not affected.
D000154:Aconitate Hydratase / Q000096:biosynthesis*; D000409:Alanine; D000469:Alkaline Phosphatase / Q000096:biosynthesis*; D000594:Amino Acid Oxidoreductases / Q000096:biosynthesis; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001386:Azides / Q000494:pharmacology; D001412:Bacillus subtilis / Q000201:enzymology*; D002474:Cell-Free System; D005969:Glutamate Dehydrogenase / Q000096:biosynthesis; D006836:Hydro-Lyases / Q000096:biosynthesis*; D006863:Hydrogen-Ion Concentration; D007521:Isocitrate Dehydrogenase / Q000096:biosynthesis; D007770:L-Lactate Dehydrogenase / Q000096:biosynthesis; D010088:Oxidoreductases / Q000096:biosynthesis*
D000900:Anti-Bacterial Agents; D001386:Azides; D010088:Oxidoreductases; D007770:L-Lactate Dehydrogenase; D007521:Isocitrate Dehydrogenase; D000594:Amino Acid Oxidoreductases; D005969:Glutamate Dehydrogenase; D000469:Alkaline Phosphatase; D006836:Hydro-Lyases; D000154:Aconitate Hydratase; D000409:Alanine