title: Studies on the source of cyclic AMP in canine gastric juice.
abstract: The possibility that cyclic AMP in gastric juice is derived from plasma by simple clearance was evaluated. The effect of exogenous cyclic AMP administration (1 mg/min) on radioimmunoassayable cyclic AMP in plasma and gastric juice was studied in dog stomach during histamine (8--16 micrograms/kg/hr) infusion. Experiments were performed in vagally denervated fundic (Heidenhain) and in innervated gastric pouches. During infusion of cyclic AMP with histamine, plasma cyclic AMP concentration rapidly increased 500-fold. Simultaneously, gastric juice cyclic AMP levels decreased almost 50%. In contrast, dibutyryl cyclic AMP infusion increased gastric juice cAMP concentration 5-fold, suggesting that the gastric mucosa is more permeable to dibutyryl cyclic AMP. In Heidenhain pouch experiments the clearance of dibutyryl cyclic AMP into gastric juice was only 1.5% of the clearance of aminopyrine. It appears that plasma clearance of cyclic AMP fails to account for most of the cyclic AMP present in gastric juice.
mesh_terms: D000632:Aminopyrine / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport; D003994:Bucladesine / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology; D000242:Cyclic AMP / Q000008:administration & dosage / Q000378:metabolism* / Q000494:pharmacology; D004285:Dogs; D005260:Female; D005750:Gastric Juice / Q000378:metabolism*; D005753:Gastric Mucosa / Q000378:metabolism*; D006632:Histamine / Q000008:administration & dosage / Q000494:pharmacology; D007263:Infusions, Parenteral; D013268:Stimulation, Chemical; D014628:Vagotomy
chemical_list: D000632:Aminopyrine; D003994:Bucladesine; D006632:Histamine; D000242:Cyclic AMP