Ability of calf brain synaptic membranes to bind [35S]taurine to their triton X-100 and chloroform extracts.
A protein fraction containing reducing sugars was prepared from calf brain synaptic membranes with 0.5% Triton X-100, and another fraction containing bound phosphorus with a 2 : 1 chloroform--methanol mixture. Both protein fractions bound small amounts of [35S]taurine, the first fraction about 25 pmol/g protein and the second about 220 pmol/g protein. The Triton X-100 extract represented 13.8% of the membrane proteins, but the chloroform--methanol extract only 0.9%. The binding of taurine to the Trition X-100 extract was temperature sensitive, but was only slightly inhibited by hypotaurine and beta-alanine.
D000409:Alanine / Q000378:metabolism; D000818:Animals; D002417:Cattle; D002457:Cell Extracts; D002540:Cerebral Cortex / Q000378:metabolism*; D002725:Chloroform; D011092:Polyethylene Glycols; D017981:Receptors, Neurotransmitter / Q000378:metabolism*; D013654:Taurine / Q000031:analogs & derivatives / Q000378:metabolism*
D002457:Cell Extracts; D017981:Receptors, Neurotransmitter; D013654:Taurine; D011092:Polyethylene Glycols; D002725:Chloroform; D000409:Alanine