SV40-transformed human diploid cells that remain transformed throughout their limited lifespan.
Of ninety-one subcultured foci of SV40-transformed WI-38 human diploid fibroblasts, none yielded cells that grew indefinitely, but all cells in each subculture continued to produce T antigen and to look morphologically transformed throughout their lifespan. These results are consistent with the commitment theory of fibroblast senescence, but predict that a special transformation event is necessary to accout for the rare survivors.
D000951:Antigens, Neoplasm / Q000032:analysis; D000956:Antigens, Viral / Q000032:analysis; D002455:Cell Division; D002460:Cell Line; D002470:Cell Survival; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D006801:Humans; D008168:Lung / Q000196:embryology; D013539:Simian virus 40 / Q000254:growth & development* / Q000276:immunology
D000951:Antigens, Neoplasm; D000956:Antigens, Viral