Studies of lactate dehydrogenase of Mycoplasma mycoides var. mycoides.
Polyacrylamide gel electrophoresis and isoelectric focusing techniques have been used to compare NAD-dependent L(plus) lactate dehydrogenases (LDH) from ten different strains of Mycoplasma mycoides var. mycoides. The enzymes were not distinguished from one another, or from normal bovine LDH 1 by these methods. The kinetic behaviour of LDH form M. mycoides (T1 vaccine strain) suggested that the enzyme could readily reduce pyruvate or oxidize lactate in a manner which, in vertebrates, requires two different isoenzymes.
D000818:Animals; D002417:Cattle; D002458:Cell Fractionation; D002474:Cell-Free System; D002645:Chickens; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D007527:Isoenzymes; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007773:Lactates / Q000378:metabolism; D009176:Mycoplasma mycoides / Q000201:enzymology*; D009243:NAD / Q000378:metabolism; D011773:Pyruvates / Q000378:metabolism; D011817:Rabbits; D013696:Temperature
D007527:Isoenzymes; D007773:Lactates; D011773:Pyruvates; D009243:NAD; D007770:L-Lactate Dehydrogenase