Analysis of viral gene functions by means of early temperature-sensitive mutants of human cytomegalovirus.
Fifteen ts mutants of CMV, which are unable to form normal plaques at 39 degrees C on HEL cells but do form plaques at 34 degrees C, have been isolated following mutagenesis by ultraviolet light. Five mutants defective in viral DNA synthesis at 39 degrees C were divided into two different complementation groups and examined for various phenotypic characteristics.
D003587:Cytomegalovirus / Q000235:genetics* / Q000378:metabolism; D004279:DNA, Viral / Q000096:biosynthesis; D004259:DNA-Directed DNA Polymerase / Q000096:biosynthesis; D003673:Defective Viruses / Q000378:metabolism; D005814:Genes, Viral; D005816:Genetic Complementation Test; D009154:Mutation; D010641:Phenotype; D013696:Temperature; D013997:Time Factors; D014779:Virus Replication
D004279:DNA, Viral; D004259:DNA-Directed DNA Polymerase