title: [Gelelectrofocusing of protektin anti AHP from the albumin gland of Helix pomatia (author's transl)].
abstract: The protektin anti AHP from the albumine gland of Helix pomatia is a very interesting substance because of its ability to agglutinate erythrocytes of blood group A as well as group C streptococci. Purification is possible by reversible absorption on Sephadex G200. Isoelectric focusing in a sucrose density gradient is difficult, because the protein precipitates at the isoelectric point. The agglutinin, which is homogeneous in disk electrophoresis experiments (in an alkaline gel system as well as in an acidic one), shows around 9 bands in gelelectrofocusing. All components are able to agglutinate group C-streptococci. Complex formation between protein and ampholytes has been excluded by experiments in gels containing 7M urea. The region of the isoelectric points of the components is between pH 6 and pH 8. The inhomogeneity in gelelectrofocusing is caused probably by differences in the subunits of the protein molecule.
mesh_terms: D000017:ABO Blood-Group System; D000371:Agglutination; D000373:Agglutinins / Q000032:analysis*; D004912:Erythrocytes / Q000276:immunology; D006372:Helix, Snails; D006388:Hemagglutinins / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing / Q000379:methods*; D013291:Streptococcus / Q000276:immunology
chemical_list: D000017:ABO Blood-Group System; D000373:Agglutinins; D006388:Hemagglutinins
vernacular_title: Gelelektrofokussierung des Protektins Anti AHP aus der Eiweissdrüse von Helix pomatia (Weinbergschnecke)