Determination of 6-methyladenine in DNA by high-performance liquid chromatography.
A method for the determination of 6-methyladenine (6MA) by high-performance liquid chromatography (HPLC) has been developed. DNA bases were separated by using the strong cation-exchange resin Zipax SCX. Purine bases were obtained by hydrolysis and dialysis of DNA and analysed by HPLC. 6MA in DNA from Escherichia coli was determined by the proposed method. It is suggested that the method could be applicable to analyses of 6MA from other biological sources.
D000225:Adenine / Q000031:analogs & derivatives* / Q000032:analysis; D000818:Animals; D002286:Carcinoma, Ehrlich Tumor; D006528:Carcinoma, Hepatocellular; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D002851:Chromatography, High Pressure Liquid; D004247:DNA; D004269:DNA, Bacterial; D004273:DNA, Neoplasm; D004926:Escherichia coli; D008099:Liver; D008113:Liver Neoplasms; D009374:Neoplasms, Experimental; D051381:Rats; D013950:Thymus Gland
D000225:Adenine; D004247:DNA; D004269:DNA, Bacterial; D004273:DNA, Neoplasm; C000730567:6-methyladenine