Viscoelastic analysis of high molecular weight, alkali-denatured DNA from mouse 3T3 cells.
Alkaline lysates of mouse 3T3 cells showed viscoelastic properties characteristic of very large molecules of single-stranded DNA. The viscoelastic retardation time and the sensitivity to low doses of nitrogen mustard and of X-irradiation suggest a molecular weight in excess of 10-10 daltons. Contact-inhibited cells yielded larger single strands than actively growing cells.
D000818:Animals; D002478:Cells, Cultured; D003260:Contact Inhibition; D004247:DNA / Q000528:radiation effects; D004277:DNA, Single-Stranded; D004548:Elasticity; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D051379:Mice; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D011830:Radiation Effects; D013997:Time Factors; D014783:Viscosity
D004277:DNA, Single-Stranded; D004247:DNA