Metabolism of cholecalciferol in land snails.
1. Radioactively labelled cholecalciferol was injected into the land snails Levantina hiersolyma and Theba pisana. Three metabolites (C, D and E), more polar than cholecalciferol, were found. 2. Metabolite C was found to be identical with 25-hydroxycholecalciferol. On injection of 25-hydroxy[26,27-3H]cholecalciferol, metabolite E was predominantly formed. Metabolite D was predominantly formed from cholecalciferol. Metabolites D and E differ from any known cholecalciferol metabolites. 3. The intestine was found to be the tissue capable of carrying out the transformation of 25-hydroxycholecalciferol into metabolite E. 4. 25-Hydroxycholecalciferol and metabolite E were localized in the digestive gland of the snail, the tissue responsible for the absorption of Ca2+ and its storage. Metabolite D was not localized in any specific tissue.
D000818:Animals; D002762:Cholecalciferol / Q000378:metabolism*; D002852:Chromatography, Ion Exchange; D006887:Hydroxycholecalciferols / Q000378:metabolism; D012908:Snails / Q000378:metabolism*; D014018:Tissue Distribution
D006887:Hydroxycholecalciferols; D002762:Cholecalciferol