Carbamylation of alkaline mesentericopeptidas.
The effects of carbamylation with potassium cyanate, and methylation with methyl p-nitrobenzene sulphonate on the mesentericopeptidase activity are studies. The treatment with potassium cyanate causes the enzyme to lose its activity towards ester substrates and casein. The specific reagent N-trans-cinnamoylimidazole does not acylate the active site in the  carbamylated enzyme. The pH dependence of the rate of inactivation indicates that an ionizing group of pK = 7.3, probably the protonated imidazole group of the active site histidine, is involved in the reaction. The competitive inhibitor boric acid protects mesentericopeptidase against inactivation with potassium cyanate. These suggest that the active site residues are modified in the unprotected enzyme. Sixty per cent of the enzyme activity toward N-acetyl-L-tyrosine ethyl ester was restored after treatment of the carbamylated mesentericopeptidase with 1 M hydroxylamine hydrochloride. Circular dichroism spectra show that the carbamylation does not change markedly the native protein conformation.
D000215:Acylation; D001407:Bacillus; D001557:Benzenesulfonates; D001888:Boric Acids; D002364:Caseins; D002942:Circular Dichroism; D003485:Cyanates; D010450:Endopeptidases; D004789:Enzyme Activation; D004952:Esters; D006639:Histidine; D006863:Hydrogen-Ion Concentration; D006898:Hydroxylamines; D007093:Imidazoles; D008745:Methylation; D009578:Nitrobenzenes; D011188:Potassium; D013997:Time Factors
D001557:Benzenesulfonates; D001888:Boric Acids; D002364:Caseins; D003485:Cyanates; D004952:Esters; D006898:Hydroxylamines; D007093:Imidazoles; D009578:Nitrobenzenes; D006639:Histidine; D010450:Endopeptidases; D011188:Potassium