Effect of adrenaline and triamcinolone on cytochrome oxidase activity in the brain and liver of young and adult rats.
The effect of adrenaline (0.15 i.p./kg b.w.) and of the synthetic glucocorticoid triamcinolone (40 mg i.p./kg b.w.) on cytochrome oxidase activity, the terminal enzyme of the cytochrome system, was studied in homogenates of the cerebral cortex, subcortical formations (including the basal ganglia, the thalamus and the hypothalamus), the medulla oblongata and the liver of 5-day-old and adult rats. Activity in the above mentioned homogenates was measured polarographically 15 and 30 min after administering adrenaline or 48 h after administering triamcinolone. Fifteen minutes after its injection, adrenaline caused a statistically significant drop in cytochrome oxidase activity in the cerebral cortex, subcortical formations and liver of 5-day-old rats. The decrease still persisted 30 min after administration of the hormone, but was intensified only in the liver. In adult rats, on the other hand, a significant increase in activity was observed in the cerebral cortex and liver after adrenaline. Triamcinolone had no effect on cytochrome oxidase activity in any of the given parts of the brain in either young or adult rats. It significantly stimulated cytochrome oxidase activity in the liver of 5-day-old rats, but severely inhibited it in the liver of adult rats.
D000367:Age Factors; D000818:Animals; D001921:Brain / Q000201:enzymology*; D002540:Cerebral Cortex / Q000201:enzymology; D003576:Electron Transport Complex IV / Q000378:metabolism*; D004837:Epinephrine / Q000494:pharmacology*; D005260:Female; D008099:Liver / Q000201:enzymology*; D008297:Male; D008526:Medulla Oblongata / Q000201:enzymology; D051381:Rats; D014221:Triamcinolone / Q000494:pharmacology*
D014221:Triamcinolone; D003576:Electron Transport Complex IV; D004837:Epinephrine