title: A simple large-scale method for separating closed circular form DNA by gel electrophoresis.
abstract: The covalently closed form of circular duplex SV40 DNA was separated from the open and linear form of SV40 DNA by agarose gel electrophoresis in a large-scale gel system. The closed circular DNA was recovered from agarose gels by re-electrophoresing the gel slices. The recovery of DNA was about 70%. Electron microscopic analysis showed that the recovered DNA did not have doube- or single-stranded breaks. The recovered DNA can be used without further purification for electron microscopy, as a substrate for experiments using restriction endonuclease and as a template for in vitro RNA synthesis.
mesh_terms: D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D004270:DNA, Circular / Q000302:isolation & purification*; D004279:DNA, Viral / Q000032:analysis / Q000302:isolation & purification*; D004586:Electrophoresis / Q000379:methods*; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D000882:Haplorhini; D007668:Kidney; D008970:Molecular Weight; D013539:Simian virus 40 / Q000032:analysis
chemical_list: D004270:DNA, Circular; D004279:DNA, Viral