Mass fragmentographic determination of cholecalciferol and 25-hydroxycholecalciferol in human serum.
The serum extracts were purified by column-, thin layer- and high pressure liquid chromatography. Deuterated cholecalciferol and deuterated 25-hydroxycholecalciferol were used in internal standards. The quantitative analysis was performed using GC-mass fragmentography technique of TMS-ethers.
D002762:Cholecalciferol / Q000097:blood*; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006887:Hydroxycholecalciferols / Q000097:blood*; D008722:Methods
D006887:Hydroxycholecalciferols; D002762:Cholecalciferol