The purification of Q-containing tRNAs by periodate modification. Purification and nucleoside composition of two related Drosophila tyrosine tRNAs.
A simple procedure for the purification of Drosophila tRNAs which contain the hypermodified nucleoside Q was developed. The cis-diol group of Q renders it susceptible to attack by sodium periodate, with the resultant formation of a hydrophobic pyrrol ring structure. The increase in hydrophobicity after periodate modification, of those tRNA species which contain the Q nucleoside, causes them to be selectively retarded on benzoylated DEAE-cellulose or RPC-5 columns, and allows their isolation in essentially pure form. Utilizing this property, the Q-containing tyrosine tRNA from Drosophila, tRNATyr1delta, was purified. The non-Q-containing tyrosine tRNA, tRNA, tRNATyr1gamma, was also purified, and the nucleoside compositions of the two were determined and compared. In addition to the Q nucleoside, these tRNAs appear to differ by the presence (tRNATyr1delta) or absence (tRNATyr1gamma) of 5-methylcytidine. In all other respects, these tRNAs appear to be identical, and are probably products of the same gene which differ in their levels of post-transcriptional modification.
D000818:Animals; D001483:Base Sequence; D002845:Chromatography / Q000379:methods; D004330:Drosophila / Q000235:genetics; D006151:Guanosine / Q000031:analogs & derivatives / Q000032:analysis; D010504:Periodic Acid; D012343:RNA, Transfer / Q000032:analysis / Q000302:isolation & purification*; D014443:Tyrosine
D010504:Periodic Acid; D006151:Guanosine; D014443:Tyrosine; D012343:RNA, Transfer