title: Effect of nitrogen starvation on the level of adenosine 3',5'-monophosphate in Anabaena variabilis.
abstract: Low levels of adenosine 3',5'-monophosphate (cyclic AMP) were detected in the cyanobacterium Anabaena variabilis using a protein binding assay and two radioisotopic labelling methods. The basal concentration of intracellular cyclic AMP ranged from 0.27 pmol/mg protein in A. variabilis Kutz grown under heterotrophic conditions to 1.0--2.7 pmol/mg protein in A. variabilis strain 377 grown autotrophically. Extracellular cyclic AMP was found to comprise as much as 90% of the total cyclic AMP in rapidly growing cultures. When A. variabilis strain 377 was starved of nitrogen, a 3--4-fold increase in intracellular cyclic AMP was observed during the 24 h period coincident with early heterocyst development.
mesh_terms: D002250:Carbon Radioisotopes; D000458:Cyanobacteria / Q000378:metabolism*; D000242:Cyclic AMP / Q000302:isolation & purification / Q000378:metabolism*; D008717:Methionine Sulfoximine / Q000494:pharmacology; D009584:Nitrogen / Q000494:pharmacology*; D010761:Phosphorus Radioisotopes
chemical_list: D002250:Carbon Radioisotopes; D010761:Phosphorus Radioisotopes; D008717:Methionine Sulfoximine; D000242:Cyclic AMP; D009584:Nitrogen