Effect of hydralazine and dihydralazine on connective tissue and binding to serum protein.
Hydralazine and dihydralazine, chelators of Fe2+, Fe3+ and Mn2+ ions, inhibited collagen biosynthesis in organ culture of chicken embryo tibiae. Both substances inhibited the in vitro hydroxylation of [14C]Pro-labelled protocollagen by protocollagen proline hydroxylase. A decreased incorporation of [14C]-D-glucosamine into glyco- and/or muco-proteins was found under the effect of the two drugs. Dihydralazine, a cationic compound, was bound to DNA and acid mucopolysaccharides (glycosaminoglycans), forming an insoluble complex. Both substances were bound to serum alpha 2-globulin. Subcutaneous injections of dihydralazine in rats produced a local as well as a general toxic effect.
D000818:Animals; D001345:Autoradiography; D001798:Blood Proteins / Q000378:metabolism*; D002642:Chick Embryo; D003094:Collagen / Q000096:biosynthesis*; D003238:Connective Tissue / Q000187:drug effects / Q000378:metabolism*; D004078:Dihydralazine / Q000097:blood / Q000494:pharmacology*; D006830:Hydralazine / Q000031:analogs & derivatives* / Q000097:blood / Q000494:pharmacology*; D005779:Immunodiffusion; D046911:Macromolecular Substances; D009924:Organ Culture Techniques; D011347:Procollagen / Q000096:biosynthesis / Q000378:metabolism; D011393:Procollagen-Proline Dioxygenase / Q000378:metabolism; D011485:Protein Binding; D051381:Rats; D012867:Skin / Q000201:enzymology / Q000378:metabolism; D013977:Tibia / Q000378:metabolism
D001798:Blood Proteins; D046911:Macromolecular Substances; D011347:Procollagen; D006830:Hydralazine; D003094:Collagen; D011393:Procollagen-Proline Dioxygenase; D004078:Dihydralazine