An effect of cell-culture passage on ultraviolet-enhanced viral reactivation by mammalian cells.
The ability of ultraviolet-irradiated African green monkey kidney cells to reactivate UV-damaged herpes simplex virus was tested over a range of 57 passages (24-81). Several types of controls were employed to minimize daily variations. The results indicate that in this system cells demonstrate an increasing efficiency to reactivate UV-damaged virus until approx. passage 60, followed by a decrease in this ability beyond this passage. Chromosome counts at selected passages revealed no significant change in chromosome number as a function of passage. The mechanism(s) or factor(s) causing this effect are unknown.
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D002875:Chromosomes / Q000528:radiation effects; D003584:Cytological Techniques; D000882:Haplorhini; D007668:Kidney; D018139:Simplexvirus / Q000254:growth & development; D013997:Time Factors; D014466:Ultraviolet Rays; D014775:Virus Activation / Q000528:radiation effects*