title: Herpesvirus papio: state of viral DNA in baboon lymphoblastoid cell lines.
abstract: Baboon lymphoblastoid cell lines (LCL) transformed with herpesvirus papio were evaluated for the state of HVP DNA in the cells by caesium chloride density-gradient centrifugation and DNA-cRNA filter hybridization. Five HVP producer cultures and one non-producer culture were studied and non-integrated and integrated HVP DNA were detected in all producer cultures whereas only integrated HVP DNA could be demonstrated in the non-producer LCL. Non-integrated HVP dna had a density of 1.715--1.716 g/cm3 in caesium chloride corresponding to a guanine + cytosine content of 56--57%.
mesh_terms: D000818:Animals; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D002499:Centrifugation, Density Gradient; D004270:DNA, Circular / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism*; D000882:Haplorhini; D006564:Herpesviridae / Q000378:metabolism*; D004854:Herpesvirus 4, Human / Q000378:metabolism; D008214:Lymphocytes; D009693:Nucleic Acid Hybridization; D010215:Papio; D014170:Transformation, Genetic
chemical_list: D004270:DNA, Circular; D004279:DNA, Viral