Ultrasensitive enzymatic radioimmunoassay: application to detection of cholera toxin and rotavirus.
Rotavirus and enterotoxin-producing bacteria are major causes of diarrheal disease in humans. A method of rapid diagnosis, ultrasensitive enzymatic radioimmunoassay, has been developed to quantitatively detect cholera toxin and rotavirus. The method uses features of both enzyme-linked immunosorbent assay and radioimmunoassay; however, the sensitivity of the assay is 100- to 1000-fold more sensitive than the two parent assays. Ultrasensitive enzymatic radioimmunoassay should also be useful in measuring other biologically important agents such as drugs and hormones.
D000469:Alkaline Phosphatase; D002772:Cholera Toxin / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008832:Microchemistry; D012328:RNA Viruses / Q000302:isolation & purification*; D011863:Radioimmunoassay / Q000379:methods; D012401:Rotavirus / Q000302:isolation & purification*
D002772:Cholera Toxin; D000469:Alkaline Phosphatase