Localization of acid phosphatase in protoplasts from Saccharomyces cerevisiae.
The localization of acid phosphatase (EC 3.1.3.2) in secreting protoplasts prepared from Saccharomyces cerevisiae is reported for the first time. Using a Gomori technique we were able to show acid phosphatase at those organelles in the protoplasts which are generally involved in the processes of biosynthesis and secretion of glycoproteins in eukaryotic cells.
D000135:Acid Phosphatase / Q000302:isolation & purification*; D002462:Cell Membrane / Q000201:enzymology; D002467:Cell Nucleus / Q000201:enzymology / Q000648:ultrastructure; D004721:Endoplasmic Reticulum / Q000201:enzymology; D006651:Histocytochemistry; D006863:Hydrogen-Ion Concentration; D008566:Membranes / Q000201:enzymology; D011523:Protoplasts / Q000201:enzymology* / Q000648:ultrastructure; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000648:ultrastructure; D014617:Vacuoles / Q000201:enzymology
D000135:Acid Phosphatase