title: Increased collagen prolyl hydroxylase activity in the aortic wall of rabbits exposed to chronic hypoxia.
abstract: The activity of collagen prolyl hydroxylase in aortic wall was studied in rabbits exposed to chronic 10% ambient oxygen tension for 30 days. Prolyl hydroxylase in rabbit aorta was shown to be similar to the enzyme from other sources in that it required molecular oxygen, alpha-ketoglutarate, ferrous iron and ascorbate for its activity. The activity of prolyl hydroxylase was increased to 180% of controls in the intima-media samples from rabbits exposed to hypoxia. No atherosclerotic lesions could be seen in arteries of animals kept in chronic hypoxia. If the arteries of rabbits were injured with a single mechanical dilatation, the activity of prolyl hydroxylase increased more than 2-fold, as reported previously. The exposure of these animals to chronic hypoxia further elevated the prolyl hydroxylase activity.
mesh_terms: D000818:Animals; D001011:Aorta / Q000201:enzymology* / Q000378:metabolism; D001161:Arteriosclerosis / Q000201:enzymology; D003094:Collagen / Q000378:metabolism*; D000860:Hypoxia / Q000201:enzymology*; D008297:Male; D011393:Procollagen-Proline Dioxygenase / Q000032:analysis*; D011817:Rabbits
chemical_list: D003094:Collagen; D011393:Procollagen-Proline Dioxygenase