[Analysis of ribosomes by polyacrylamide gel electrophoresis (author's transl)].
Ribosomal polymers, monomers and subunits from several eukaryotes and prokaryotes were isolated and analyzed by polyacrylamide gel electrophoresis. Extraction of RNA from ribosomal particles after their migration in a polyacrylamide gel, analyses by sedimentation in sucrose gradients and observations in the electron microscope were carried out in parallel. Attention was directed to the reproducibility, the precision and the limitations of the electrophoresis technique.
D000818:Animals; D002708:Chlorella / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005056:Euglena gracilis / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008274:Magnesium; D008854:Microscopy, Electron; D009994:Osmolar Concentration; D012269:Ribosomal Proteins / Q000032:analysis; D012270:Ribosomes / Q000032:analysis / Q000648:ultrastructure*; D012964:Sodium; D013045:Species Specificity; D013769:Tetrahymena pyriformis / Q000032:analysis
D012269:Ribosomal Proteins; D012964:Sodium; D008274:Magnesium
Analyse de ribosomes par électrophorèse en gel de polyacrylamide