Control of lysine biosynthesis in Bacillus subtilis: inhibition of diaminopimelate decarboxylase by lysine.
Diaminopimelate decarboxylase has been characterized in extracts of Bacillus subtilis and resolved from aspartokinases I and II. Under certain conditions, the enzyme is specifically inhibited by physiological concentrations of L-lysine, but less specificity and altered kinetics of inhibition are observed if lower ionic strengths are employed in the assay procedure. Diaminopimelate decarboxylase can be desensitized to lysine inhibition by either lowering the pH or diluting the enzyme in Tris buffer in the absence of pyridoxal phosphate. Evidence is presented to incidate that, under proper conditions, lysine inhibition involves an interaction of the amino acid with the enzyme rather than competition for available pyridoxal phosphate in the assay. Lysine, by affecting the level of meso-diaminopimelate, may thus regulate its biosynthesis through  sequential feedback inhibition. Analysis of the diaminopimelate decarboxylase of 15 revertants of mutants that had originally lacked diaminopimelate decarboxylase activity indicates that as little as 5% of the specific activity of enzyme observed in the wild-type strain is sufficient to permit normal growth rates. In the growing cell, diaminopimelate decarboxylase may therefore exist largely in an inhibited state.
D001224:Aspartic Acid; D001412:Bacillus subtilis / Q000201:enzymology* / Q000378:metabolism; D002250:Carbon Radioisotopes; D002262:Carboxy-Lyases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D002474:Cell-Free System; D002850:Chromatography, Gel; D003959:Diamines / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007527:Isoenzymes / Q000378:metabolism; D008239:Lysine / Q000096:biosynthesis / Q000494:pharmacology*; D010710:Phosphates / Q000494:pharmacology; D010761:Phosphorus Radioisotopes; D010770:Phosphotransferases / Q000378:metabolism; D010867:Pimelic Acids / Q000378:metabolism; D011732:Pyridoxal Phosphate / Q000494:pharmacology; D013237:Stereoisomerism; D014325:Tromethamine / Q000494:pharmacology
D002250:Carbon Radioisotopes; D003959:Diamines; D007527:Isoenzymes; D010710:Phosphates; D010761:Phosphorus Radioisotopes; D010867:Pimelic Acids; D014325:Tromethamine; D001224:Aspartic Acid; D011732:Pyridoxal Phosphate; D010770:Phosphotransferases; D002262:Carboxy-Lyases; D008239:Lysine