Comparison between muscle and liver enolases and their behavior during differentiation and growth.
1. Rabbit liver enolase (EC 4.2.1.11) was purified about 200-fold and the enzyme was distinguished from crystalline muscle enolase by column isoelectrofocusing.  It was found that the pI of muscle enolase was at about pH 8.8 and the pI of liver enolase was at about pH 6.7.  Liver enolase was more liable to heat than muscle enolase.  Anti-muscle enolase antibody did not react with liver enolase in double diffusion and immunoprecipitation tests.  No substantial  difference seemed to exist between muscle and liver enolases in pH optima, kinetic constants, and gel filtration. 2. It was observed by electrofocusing that the pI of rat muscle enolase was pH 7.2 to 7.9 and that of liver enolase was about pH 5.9.  The main component of muscle enolase was designated as type A enolase, and liver enolase as type B enolase.  Type A enolase was present in skeletal muscle and heart muscle.  Type B enolase was widely distributed and present in liver, kidney, spleen, brain, lung, small intestine, and heart muscle.  More acidic isozyme than type B enolase coexisted in the brain, and more basic isozyme than type A enolase, coexisted in the small intestine.  A prototype of enolase in the early stage of differentiation was found to be type B enolase and, as differentiation progressed, type B decreased in muscle, while type A increased.  On the other hand, liver enolase was retained as type B during differentiation.  The enolase in regenerating liver was the same as in normal liver.
D000818:Animals; D002454:Cell Differentiation; D005591:Chemical Fractionation; D002850:Chromatography, Gel; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D005779:Immunodiffusion; D007525:Isoelectric Focusing; D007700:Kinetics; D008099:Liver / Q000201:enzymology* / Q000254:growth & development; D008115:Liver Regeneration; D024510:Muscle Development; D009132:Muscles / Q000201:enzymology*; D009206:Myocardium / Q000201:enzymology; D010751:Phosphopyruvate Hydratase / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D011817:Rabbits; D051381:Rats
D010751:Phosphopyruvate Hydratase