The inability of nuclear dehydrogenating clostridia to oxidize bile salt hydroxyl groups.
In a survey of the intracellular bile salt oxidoreductase activity in fecal bacteria, 16 strains of nuclear dehydrogenating clostridia and 2 strains of non-nuclear dehydrogenating C. paraputrificum were demonstrated unable to oxidize cholate at any of the 3 OH groups. Since nuclear dehydrogenation at the delta-1 and delta-4 position requires a 3-oxo precursor steroid, it appears that these organisms require the presence of a 3 alpha-hydroxysteroid dehydrogenating organism for nuclear dehydrogenation.
D001647:Bile Acids and Salts / Q000378:metabolism*; D003013:Clostridium / Q000201:enzymology*; D005243:Feces / Q000382:microbiology; D006801:Humans; D006913:Hydroxysteroid Dehydrogenases / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism
D001647:Bile Acids and Salts; D009243:NAD; D010088:Oxidoreductases; D006913:Hydroxysteroid Dehydrogenases