title: Organ culture of adult mouse intestine. I. Morphological results after 24 and 48 hours of culture.
abstract: Explants of adult mouse intestine have been maintained in organ culture for 24 to 48 hr. The best results have been obtained with a mixture of DMEM-HEPES medium and NCTC-135 enriched with 10% fetal bovine serum. The morphology of the mucosa is well preserved at the light and electron microscopic level: absorbing cells exhibit an increase in secondary lysosomes; goblet cells and Paneth cells remain active; numerous mitoses are observed in the crypts; and vigorous re-epithelization takes place on the margin of the explants.
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D003470:Culture Media; D004386:Duodenum / Q000166:cytology*; D007413:Intestinal Mucosa / Q000166:cytology*; D007583:Jejunum / Q000166:cytology*; D008214:Lymphocytes / Q000166:cytology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D008871:Microvilli / Q000648:ultrastructure; D008938:Mitosis; D009924:Organ Culture Techniques; D009940:Organoids / Q000648:ultrastructure; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis
chemical_list: D003470:Culture Media; D010744:Phosphoric Monoester Hydrolases