Coenzyme specificity in the glycolysis of intraocular fluids.
Toward elucidation of the glycolysis in the vitreous and aqueous of several animal species, coenzymes of this scheme, NAD, NADH and ATP were assayed but found to be virtually absent. To further assess this pathway, ADP and NADPH in addition to the above were introduced singly and in combination with 0.20 ml-aliquots of the guinea pig aqueous and vitreous under anaerobic conditions and the absorbance followed spectrophotometrically at 340 nm. Only NADH produced an immediate observable response and the rate of change was greater for the virteous. The action of oxamate and iodacetate was also screened. The experiments point to the LDH-catalyzed step as responsible for the observed effect.
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000255:Adenosine Triphosphate / Q000378:metabolism / Q000494:pharmacology; D000693:Anaerobiosis; D000818:Animals; D001082:Aqueous Humor / Q000201:enzymology*; D002417:Cattle; D005123:Eye / Q000201:enzymology*; D006019:Glycolysis / Q000187:drug effects; D006168:Guinea Pigs; D066298:In Vitro Techniques; D007461:Iodoacetates / Q000494:pharmacology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008253:Macaca mulatta; D009243:NAD / Q000378:metabolism / Q000494:pharmacology; D009249:NADP / Q000494:pharmacology; D011817:Rabbits; D051381:Rats; D013045:Species Specificity; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors; D014822:Vitreous Body / Q000201:enzymology*
D007461:Iodoacetates; D009243:NAD; D009249:NADP; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate; D007770:L-Lactate Dehydrogenase