Analysis of an HL-A antiserum by iso-electric focusing.
An anti-HL-A 3 antiserum with cross-reacting activity for HL-A 1 and HL-A 11 was subjected to isoelectric focusing over a pH 5-8 gradient. The cytotoxic activity of the serum focused into three distinct peaks, one at the basic end of the gradient, one between pH 6 and pH 7 and one at the acidic end. The first and second peaks reacted with HL-A 3 positive cells and HL-A 3 negative cells positive for cross-reacting antigens. The third peak reacted only with HL-A 3 positive cells.
D000918:Antibody Specificity; D003429:Cross Reactions; D003601:Cytotoxicity Tests, Immunologic; D006680:HLA Antigens / Q000032:analysis*; D006649:Histocompatibility Antigens / Q000032:analysis*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007106:Immune Sera / Q000032:analysis*; D007115:Immunization Schedule; D007525:Isoelectric Focusing; D008214:Lymphocytes / Q000276:immunology
D006680:HLA Antigens; D006649:Histocompatibility Antigens; D007106:Immune Sera