Solubilization of valine-sensitive acetohydroxy acid synthetase from Neurospora mitochondria.
Acetohydroxy acid synthetase [EC 4.1.3.18] of Neurospora crassa has been solubilized from a mitochondrial pellet fraction by sonic treatment in 1.0 M potassium phosphate buffer at pH 7.5 containing 10 mM MgSO4 and centrifugation at 180,000 X g for 20 min. The soluble enzyme thus obtained has a high specific activity and a high sensitivity to valine comparable to the original mitochondrial pellet suspension when the activity was determined in high concentrations of potassium phosphate.
D000095:Acetolactate Synthase / Q000302:isolation & purification* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008928:Mitochondria / Q000201:enzymology*; D009491:Neurospora / Q000201:enzymology*; D009492:Neurospora crassa / Q000201:enzymology*; D009994:Osmolar Concentration; D007652:Oxo-Acid-Lyases / Q000302:isolation & purification*; D010710:Phosphates / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D012995:Solubility; D013010:Sonication; D013997:Time Factors; D014461:Ultracentrifugation; D014633:Valine / Q000494:pharmacology*
D010710:Phosphates; D000095:Acetolactate Synthase; D007652:Oxo-Acid-Lyases; D014633:Valine; D011188:Potassium