[Concentration and purification of the infectious bovine rhinotracheitis virus (IBR)].
Various combinations of the method for the concentration and purification of the IBR virus have been tested. An easily applicable pattern is adopted with whose aid the virus is rapidly purified with a slightly reduced infectability. The pattern includes concentration of the virus through precipitation with polyethylenglycol, gel-filtration on a Sepharose 4B column and ultra-centrifugation in a linear gradient of sucrose. The virus is checked electronmicroscopically, immunoelectrophoretically and through a bioassay on a rabbit. When a rabbit is hyperimmunized with a virus thus purified, a high titre antiserum is obtained in which antibodies against bovine protein are lacking.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D007242:Herpesvirus 1, Bovine / Q000302:isolation & purification* / Q000472:pathogenicity; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D008722:Methods; D008854:Microscopy, Electron; D011817:Rabbits; D014461:Ultracentrifugation
D000914:Antibodies, Viral
Kontsentrirane i prechistvane na virusa na infektsiozniia rinotrakheit po govedata (IBR).