Vascular permeability-increasing action of hypoalbuminemic substance from Ehrlich ascites carcinoma cells.
A hypoalbuminemic substance (F-3), which was obtained from Ehrlich ascites carcinoma cells, showed a marked vascular permeability-increasing action in mice. The highest increasing effect was produced 20 min after an intradermal injection of F-3. This action was inhibited by diisopropyl fluorophosphate, soybean trypsin inhibitor, and indomethacin but not by hydrocortisone or D-2-bromolysergic acid diethylamide. The inhibition of F-3 by diisopropyl fluorophosphate was observed in partial even after the excess diisopropyl fluorophosphate was removed from the diisopropyl fluorophosphate-treated F-3. The hypoalbuminemic and hypoproteinemic actions were not subjected to any inhibition by the diisopropyl fluorophosphate treatment. The decrease in albumin produced 3 hr after the injection of F-3 would be due to the leakage of albumin from blood vessels. F-3 might contain at least two components, one being a vascular permeability-increasing substance and the other a hypoalbuminemic one.
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D002199:Capillary Permeability / Q000187:drug effects*; D002286:Carcinoma, Ehrlich Tumor / Q000032:analysis*; D003864:Depression, Chemical; D006854:Hydrocortisone / Q000494:pharmacology; D007213:Indomethacin / Q000494:pharmacology; D007531:Isoflurophate / Q000494:pharmacology; D007610:Kallikreins / Q000494:pharmacology; D008238:Lysergic Acid Diethylamide / Q000494:pharmacology; D008297:Male; D051379:Mice; D010447:Peptide Hydrolases / Q000494:pharmacology; D012709:Serum Albumin / Q000378:metabolism*; D013268:Stimulation, Chemical; D014020:Tissue Extracts / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D014357:Trypsin / Q000494:pharmacology; D014361:Trypsin Inhibitors / Q000494:pharmacology
D000893:Anti-Inflammatory Agents; D012709:Serum Albumin; D014020:Tissue Extracts; D014361:Trypsin Inhibitors; D007531:Isoflurophate; D008238:Lysergic Acid Diethylamide; D010447:Peptide Hydrolases; D007610:Kallikreins; D014357:Trypsin; D006854:Hydrocortisone; D007213:Indomethacin