Isolation and functional characterization of hemoglobin Casper: beta106(G8) Leu replaced by Pro.
Hemoglobin Casper (beta106Leu replaced by Pro) can be separated from hemoglobin (Hb) A by isoelectric focusing on polyacrylamide gel. This abnormal hemoglobin was estimated to be 30% of teh total by both isoelectric focusing and heat lability kinetics. Its oxygen equilibrium curves indicate a high oxygen affinity, low degree of subunit interaction, and a decreased Bohr effect. Mixtures of Hb Casper and Hb A appear to bind oxygen as if no hybrid molecules exist.
D004163:Diphosphoglyceric Acids / Q000097:blood; D004739:England; D004912:Erythrocytes / Q000378:metabolism; D006455:Hemoglobins, Abnormal / Q000302:isolation & purification; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing; D007930:Leucine; D010100:Oxygen / Q000097:blood; D011392:Proline
D004163:Diphosphoglyceric Acids; D006455:Hemoglobins, Abnormal; D011392:Proline; D007930:Leucine; D010100:Oxygen