title: [Physicochemical properties of the Okhotsky virus].
abstract: Purified concentrated Okhotsky virus was prepared. The buoyant densities of its virions were determined in sucrose and cesium chloride gradients and found to be 1.16--1.18 and 1.36 g/cm3, respectively. The experimental evidence indicated the double-stranded nature of Okhotsky virus genome RNA. The study of the above physico-chemical properties confirmed the appurtenance of Okhotsky virus to the genus Orbivirus of the Reoviridae family.
mesh_terms: D000818:Animals; D002499:Centrifugation, Density Gradient; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D005814:Genes, Viral; D051379:Mice; D018163:Orbivirus / Q000032:analysis* / Q000302:isolation & purification; D012367:RNA, Viral / Q000032:analysis / Q000302:isolation & purification; D012087:Reoviridae / Q000032:analysis*; D014776:Virus Cultivation / Q000379:methods
chemical_list: D012367:RNA, Viral
vernacular_title: Nekotorye fiziko-khimicheskie svoĭstva virusa Okhotskiĭ.