[Study of the transmembrane potential of mitochondria using cyanine dyes].
Changes in fluorescent intensity of cyanine dyes dis-C3-(5) and dis-C2-(5) were measured in suspension of rat liver mitochondria upon the development of the energized state by adding succinate or ATP. It has been shown that these dyes possess a rotenone-like effect but they do not damage the inner mitochondrial membrane. The data on ATP synthesis and Ca2+ transport correspond to the basic aspects of the chemiosmotic conception.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D004396:Coloring Agents / Q000378:metabolism*; D005453:Fluorescence; D066298:In Vitro Techniques; D008564:Membrane Potentials; D008930:Mitochondria, Liver / Q000378:metabolism*; D009243:NAD / Q000378:metabolism; D051381:Rats
D004396:Coloring Agents; D009243:NAD; D000255:Adenosine Triphosphate
Issledovaniia transmembrannogo potentsiala mitokhondriĭ s pomoshch'iu tsianinovykh krasiteleĭ.