High-affinity binding of [3H]muscimol to subcellular particles of a neurone-enriched culture of embryonic rat brain.
High-affinity, Na+-independent binding of [3H]muscimol (KB approximately equal to 1.6 x 10(-8) M; Bmax approximately equal to 0.14 nmol/g pellet) occurred to a frozen-thawed particulate fraction of 74-h-old neurone-enriched cultures prepared from the cerebra of 12-13-day-old rat embryos. This finding provides evidence that GABA-receptors exist on cultured neurones which contain only a few synaptic connections.
D000818:Animals; D001921:Brain / Q000196:embryology* / Q000378:metabolism; D001923:Brain Chemistry; D046508:Culture Techniques; D009118:Muscimol / Q000378:metabolism*; D010080:Oxazoles / Q000378:metabolism*; D051381:Rats; D017981:Receptors, Neurotransmitter / Q000032:analysis*; D013347:Subcellular Fractions / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D010080:Oxazoles; D017981:Receptors, Neurotransmitter; D009118:Muscimol; D005680:gamma-Aminobutyric Acid