Study of a haploid yeast strain with an unusually high rDNA content. II. Fractionation of the DNA in preparative Ag+-Cs2SO4 density gradients.
Yeast DNA has been fractionated in preparative Ag+-C-S2SO4 density gradients. After complexing with silver ions at a pH of about 7, the rDNA appears in a defined heavy satellite component in the gradient. For our particular strain, the satellite represents about 15% of the total nuclear DNA and has been  identified as the gamma-DNA. In alkaline CsCl density gradients, the satellite DNA forms two bands, of which the light component hybridizes with ribosomal RNA.
D002467:Cell Nucleus / Q000032:analysis; D002499:Centrifugation, Density Gradient; D002586:Cesium; D004247:DNA / Q000302:isolation & purification*; D006238:Haploidy; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D012335:RNA, Ribosomal; D012441:Saccharomyces cerevisiae / Q000032:analysis* / Q000166:cytology; D012834:Silver; D013431:Sulfates
D012335:RNA, Ribosomal; D013431:Sulfates; D002586:Cesium; D012834:Silver; D004247:DNA