Exchange of phospholipids between unilamellar vesicles of 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine and plasma very low density lipoproteins.
Purified phosphatidylcholine exchange protein from bovine liver was used to exchange [14C]dipalmitoyl phosphatidylcholine from sonicated vesicles to human plasma very low density lipoproteins (VLDL). The exchange of [14C]-dipalmitoyl phosphatidylcholine for VLDL phospholipids was temperature dependent and linear with respect to time and amount of exchange protein. In the absence of the exchange protein, less than 10% of the [14C]dipalmitoyl phosphatidylcholine was transferred. At an initial weight ratio of [14C]-dipalmitoyl phosphatidylcholine vesicles to VLDL phospholipid (1.2 mg) of 2.2, the exchange protein (14 microgram) replaced 55% of the VLDL phospholipids with [14C]dipalmitoyl phosphatidylcholine in 15 min; VLDL protein and cholesterol content were unaltered. From these studies we conclude that the exchange protein is a useful method to alter the phospholipid composition of VLDL under conditions such that there is minimal perturbation of the lipoprotein.
D000727:Androgen-Binding Protein; D000818:Animals; D002352:Carrier Proteins; D002417:Cattle; D006801:Humans; D008079:Lipoproteins, VLDL / Q000097:blood*; D008567:Membranes, Artificial; D008862:Microsomes, Liver / Q000378:metabolism; D010713:Phosphatidylcholines; D049488:Phosphatidylethanolamine Binding Protein; D049294:Phospholipid Transfer Proteins; D010743:Phospholipids / Q000097:blood*; D011663:Pulmonary Surfactants / Q000097:blood*; D013696:Temperature
D000727:Androgen-Binding Protein; D002352:Carrier Proteins; D008079:Lipoproteins, VLDL; D008567:Membranes, Artificial; C511482:PEBP1 protein, human; D010713:Phosphatidylcholines; D049488:Phosphatidylethanolamine Binding Protein; D049294:Phospholipid Transfer Proteins; D010743:Phospholipids; D011663:Pulmonary Surfactants