Studies on the stability of rat testis membrane receptors for hLH, hCG and hFSH.
Receptor preparations from rat testes were stored at 4 C with various protease inhibitors and chemicals, at -20 C and -80 C, in freeze-dried form as well as at 4 C and 37 C after reconstitution in various media. Each preparation was tested for stability in terms of binding capacity of human luteinizing hormone (hLH), human chorionic gonadotropin (hCG) and human follicle-stimulating hormone (hFSH). The freeze-dried samples were stable for at least six months, although once reconstituted and stored at 4 C, they were less stable than fresh ones. Furthermore, Na azide and Na azide with diisopropylfluoro-phosphate (DIFP) prolonged the stability of the fresh samples. The samples stored at -20 C or -80 C were stable for at least 90 days and repeated freezing and thawing (15 times) did not change the binding capacity of either the hCG/hLH or hFSH receptors.
D000818:Animals; D001386:Azides / Q000494:pharmacology; D006063:Chorionic Gonadotropin / Q000378:metabolism*; D004355:Drug Stability; D005640:Follicle Stimulating Hormone / Q000378:metabolism*; D007531:Isoflurophate / Q000494:pharmacology; D007700:Kinetics; D007986:Luteinizing Hormone / Q000378:metabolism*; D008297:Male; D011480:Protease Inhibitors / Q000494:pharmacology; D051381:Rats; D011956:Receptors, Cell Surface / Q000187:drug effects / Q000378:metabolism*; D013737:Testis / Q000187:drug effects / Q000378:metabolism*; D013997:Time Factors
D001386:Azides; D006063:Chorionic Gonadotropin; D011480:Protease Inhibitors; D011956:Receptors, Cell Surface; D007531:Isoflurophate; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone