Characterization of soluble and microsomal adenosine 3',5'-monophosphate-dependent protein kinases from rabbit heart.
Cardiac microsomes contained an intrinsic adenosine 3',5'-monophosphate (cyclic AMP)-dependent protein kinase which stimulated phosphorylation of serine residue(s) of microsomal protein. The phosphorylated residues were associated with a microsomal protein component of 20,000 molecular weight as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Intrinsic phosphoprotein phosphatase activity of the microsomal membrane resulted in rapid dephosphorylation of these residues. Microsomes phosphorylated in the presence of cyclic AMP (10(-6) M) exhibited enhanced calcium uptake. We conclude that: 1) cardiac microsomes contain intrinsic cyclic AMP-dependent protein kinase(s) which phosphorylate a specific microsomal protein and phosphoprotein phosphatase(s) capable of dephosphorylating this protein, 2) phosphorylation of this protein enhances calcium uptake, 3) reversible phosphorylation of microsomal membrane may be an important mechanism for the regulation of calcium uptake of cardiac microsomes by cyclic AMP.
D000818:Animals; D001665:Binding Sites; D001692:Biological Transport; D002118:Calcium / Q000378:metabolism; D000242:Cyclic AMP / Q000378:metabolism / Q000494:pharmacology; D006152:Cyclic GMP / Q000494:pharmacology; D003600:Cytosol / Q000201:enzymology; D004789:Enzyme Activation; D006863:Hydrogen-Ion Concentration; D008673:Metals, Alkaline Earth / Q000494:pharmacology; D008861:Microsomes / Q000201:enzymology; D009124:Muscle Proteins / Q000378:metabolism; D009206:Myocardium / Q000201:enzymology* / Q000648:ultrastructure; D010750:Phosphoproteins / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism*; D011817:Rabbits; D012995:Solubility
D008673:Metals, Alkaline Earth; D009124:Muscle Proteins; D010750:Phosphoproteins; D000242:Cyclic AMP; D011494:Protein Kinases; D010744:Phosphoric Monoester Hydrolases; D006152:Cyclic GMP; D002118:Calcium