Simplified radioimmunoassay for detection of human rotavirus in stools.
A simplified radioimmunoassay (RIA) technique was developed to facilitate the diagnosis of human rotavirus in stools of infants with diarrhea. This microtiter solid-phase RIA utilizes as a critical reagent hyperimmune serum against a tissue culture-grown simian rotavirus that is immunologically closely related to the human agent. One hundred thirteen pediatric stool samples were tested. Thiry-five specimens known to be positive for rotavirus by electron microscopy were also positive by RIA. All RIA-positive reactions (36) were shown to be specific for rotavirus by means of an antibody-blocking test. Nonspecific RIA-positive reactions were not encountered. This accurate, sensitive RIA technique is practical for use in diagnostic laboratories because critical serum reagents are prepared against culture-grown virus and not against rotavirus derived from carefully selected stools of humans or experimentally infected gnotobiotic animals.
D000818:Animals; D002675:Child, Preschool; D003968:Diarrhea, Infantile / Q000175:diagnosis / Q000382:microbiology*; D005069:Evaluation Studies as Topic; D005243:Feces / Q000382:microbiology*; D006168:Guinea Pigs; D000882:Haplorhini; D006801:Humans; D007106:Immune Sera; D007074:Immunoglobulin G; D007202:Indicators and Reagents; D007223:Infant; D007457:Iodine Radioisotopes; D007553:Isotope Labeling; D012328:RNA Viruses / Q000302:isolation & purification*; D011863:Radioimmunoassay / Q000379:methods*; D012401:Rotavirus / Q000302:isolation & purification*; D014777:Virus Diseases / Q000175:diagnosis / Q000382:microbiology*
D007106:Immune Sera; D007074:Immunoglobulin G; D007202:Indicators and Reagents; D007457:Iodine Radioisotopes