title: Cholesterol metabolism by Mycobacterium.
abstract: Cholesterol metabolism by Mycobacterium species ATCC Number 19652 was studied in defined media. Whole cells were found to take up 91% of the total cholesterol when incubated five days at 34 degrees C in media of pH 6.8-7.4. Uptake of cholesterol by whole cells could be significantly inhibited by 2,4-dinitrophenol and dicyclohexylcarbodiimide. Growth media supernates as well as isolated microbial cell walls were found to contain cholesterol hydrolysing activity. This activity was extractable by Triton X-100 and appeared to have a molecular weight of approximately 100-200,000.
mesh_terms: D002473:Cell Wall / Q000201:enzymology / Q000378:metabolism / Q000648:ultrastructure; D002784:Cholesterol / Q000378:metabolism*; D003864:Depression, Chemical; D004024:Dicyclohexylcarbodiimide / Q000494:pharmacology; D004140:Dinitrophenols / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D006867:Hydrolases / Q000378:metabolism; D009161:Mycobacterium / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D004140:Dinitrophenols; D004024:Dicyclohexylcarbodiimide; D002784:Cholesterol; D006867:Hydrolases