Production of Ficoll, Percoll, and albumin gradients by the freeze-thaw method.
Ficoll, sucrose, albumin, and Percoll, a modified colloidal silica centrifugation medium, all form density gradients upon freezing and thawing. The data suggest that any density gradient material will form gradients upon freezing and thawing, provided the material is stable to freezing.
D002499:Centrifugation, Density Gradient / Q000379:methods*; D003102:Colloids; D005362:Ficoll; D005615:Freezing; D011134:Polysaccharides; D011205:Povidone; D012709:Serum Albumin; D012822:Silicon Dioxide; D013395:Sucrose
D003102:Colloids; D011134:Polysaccharides; D012709:Serum Albumin; D005362:Ficoll; D013395:Sucrose; C016039:Percoll; D012822:Silicon Dioxide; D011205:Povidone