Effects of superoxide radicals on transport (Na + K) adenosine triphosphatase and protection by superoxide dismutase.
Membrane (Na +K)ATPase isolated from rat brain was preincubated in a medium in which superoxide radicals were generated enzymatically. Exposure to superoxide radicals caused an irreversible inactivation, which could be prevented by further addition of superoxide dismutase. (Na + K)ATPase was also protected by addition of allopurinol, a xanthine oxidase inhibitor, during preincubation. The K-activated nitrophenylphosphatase associated with (Na + K)ATPase was also found to be inactivated by preincubation with superoxide radicals, which could be prevented by superoxide dismutase.
D000493:Allopurinol / Q000494:pharmacology; D000818:Animals; D001921:Brain / Q000201:enzymology*; D007700:Kinetics; D010100:Oxygen / Q000494:pharmacology*; D051381:Rats; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism*; D013481:Superoxides / Q000494:pharmacology*; D014969:Xanthine Oxidase / Q000378:metabolism
D013481:Superoxides; D000493:Allopurinol; D013482:Superoxide Dismutase; D014969:Xanthine Oxidase; D000254:Sodium-Potassium-Exchanging ATPase; D010100:Oxygen