title: Preferential charging of tRNA-Met-f in Escherichia coli K12.
abstract: The charging of tRNA-Met-f and tRNA-Met-m in vivo and in vitro and initiation of polysomes during methionine limitation were studied in two strains of Escherichia coli K12. In the wild-type strain the distribution of polysomes as well as the kinetic parameters of methionyl-tRNA synthetase indicate preferential acylation of tRNA-Met-f. This preferential charging of tRNAM-et-f does not take place in a mutant strain which is also defective in initiation of polysomes during methionine limitation.
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000596:Amino Acids / Q000494:pharmacology; D011756:Diphosphates / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D007700:Kinetics; D008715:Methionine / Q000031:analogs & derivatives* / Q000378:metabolism*; D008718:Methionine-tRNA Ligase / Q000378:metabolism; D009154:Mutation; D009239:N-Formylmethionine / Q000378:metabolism*; D011132:Polyribosomes / Q000378:metabolism; D012343:RNA, Transfer / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D011756:Diphosphates; D009239:N-Formylmethionine; D000255:Adenosine Triphosphate; D012343:RNA, Transfer; D008715:Methionine; D008718:Methionine-tRNA Ligase