In vitro cultivation of human tumor tissues. II. Morphological and virological characterization of three cell lines.
Nineteen human tumors, mostly of sarcomatous nature, were cultured in vitro. Three cell lines were isolated and further characterized: MG-57 derived from a giant cell tumor, MG-63 derived from an osteosarcoma and MG-72 derived from a xanthohistiocytoma. The cell lines varied in morphology and growth pattern. An abnormal karyotype with marker chromosomes was present in Mg-63 and MG-72. None of the cell lines spontaneously produced detectable C-type virus particles. Stimulation with IUDR and dexamethasone also failed to induce detectable particle release.
D000818:Animals; D002460:Cell Line; D002869:Chromosome Aberrations; D005870:Giant Cell Tumors / Q000473:pathology; D018219:Histiocytoma, Benign Fibrous / Q000473:pathology; D006801:Humans; D009374:Neoplasms, Experimental / Q000235:genetics / Q000382:microbiology / Q000473:pathology*; D012190:Retroviridae / Q000302:isolation & purification; D012513:Sarcoma, Experimental / Q000473:pathology