title: The nitrate-reducing enzyme system of Chlamydomonas reinhardii.
abstract: In Chlamydomonas reinhardii the reduction of nitrate to ammonia occurs in two independent enzymatic steps: 1. the two-electrons reduction of nitrate to nitrite catalyzed by NADH-nitrate reductase, and, 2. the six-electrons reduction of nitrite to ammonia catalyzed by ferredoxin-nitrite reductase. Both enzymes have been purified and characterized, and some of their properties have been studied.
mesh_terms: D001386:Azides / Q000494:pharmacology; D002474:Cell-Free System / Q000201:enzymology; D002696:Chlamydomonas / Q000201:enzymology*; D002704:Chlorates; D003486:Cyanides / Q000494:pharmacology; D008058:Dihydrolipoamide Dehydrogenase / Q000378:metabolism; D004227:Dithionite; D005486:Flavin Mononucleotide; D005182:Flavin-Adenine Dinucleotide; D006358:Hot Temperature; D006902:Hydroxymercuribenzoates / Q000494:pharmacology; D007700:Kinetics; D008970:Molecular Weight; D009243:NAD; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D009249:NADP; D009565:Nitrate Reductases / Q000378:metabolism*; D009572:Nitrite Reductases / Q000037:antagonists & inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D010084:Oxidation-Reduction; D010618:Phenanthrolines / Q000494:pharmacology; D014755:Viologens
chemical_list: D001386:Azides; D002704:Chlorates; D003486:Cyanides; D006902:Hydroxymercuribenzoates; D010618:Phenanthrolines; D014755:Viologens; D009243:NAD; D005182:Flavin-Adenine Dinucleotide; D004227:Dithionite; D009249:NADP; D005486:Flavin Mononucleotide; D009247:NADH, NADPH Oxidoreductases; D009565:Nitrate Reductases; D009572:Nitrite Reductases; D008058:Dihydrolipoamide Dehydrogenase