Rat liver cysteine sulfinate decarboxylase: purification, new appraisal of the molecular weight and determination of catalytic properties.
Rat liver cystein sulfinate decarboxylase (L-cystein sulfinate carboxylase) was purified approximately 500-fold. By cellulose acetate and polyacrylamide gel electrophoresis or by analytical ultracentrifugation, the purified enzyme appears to be nearly homogeneous. The Stokes radius (3.4 nm) and sedimentation coefficient (6.5 S) were determined. The molecular weight, calculated and experimentally estimated is around 100 000 and the enzyme is constituted of two identical subunits whose molecular weights are 55 000. The role of pyridoxal phosphate as coenzyme was demonstrated and the requirement for free sulhydryl groups for activity was studied. The ability of native pure cysteine sulfinate decarboxylase to also decarboxylate cysteate was stressed: therefore, we concluded that in rat liver a single protein catalyzed both reactions, although only the decarboxylation of cysteine sulfinate is of physiological interest.
D000818:Animals; D001894:Borohydrides / Q000494:pharmacology; D002262:Carboxy-Lyases / Q000302:isolation & purification / Q000378:metabolism*; D003545:Cysteine; D004229:Dithiothreitol / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008297:Male; D008970:Molecular Weight; D009638:Norepinephrine / Q000494:pharmacology; D010396:Penicillamine / Q000494:pharmacology; D011487:Protein Conformation; D011732:Pyridoxal Phosphate / Q000494:pharmacology; D051381:Rats; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013441:Sulfinic Acids; D013696:Temperature
D001894:Borohydrides; D013438:Sulfhydryl Compounds; D013441:Sulfinic Acids; D011732:Pyridoxal Phosphate; D002262:Carboxy-Lyases; D010396:Penicillamine; D003545:Cysteine; D004229:Dithiothreitol; D009638:Norepinephrine