An improved procedure for purifying RNA tumor viruses from malignant tissue.
A convenient method for purifying RNA tumor viruses is described. Viruses banded in isopycnic gradients are contaminated with membranes that can be removed by velocity sedimentation in glycerol gradients. On sodium dodecyl sulfate (SDS) gel electrophoresis, the particles purified by these procedures from mouse mammary tumor tissue show protein profiles typical of those observed with virus purified from milk.
D000818:Animals; D002500:Centrifugation, Isopycnic / Q000379:methods; D008325:Mammary Neoplasms, Experimental / Q000382:microbiology; D008324:Mammary Tumor Virus, Mouse / Q000302:isolation & purification; D051379:Mice; D009858:Oncogenic Viruses / Q000302:isolation & purification*; D012328:RNA Viruses / Q000302:isolation & purification*; D014412:Tumor Virus Infections / Q000382:microbiology