The wavelength dependence of 8-methoxypsoralen photosensitization of radiation-enchanced reactivation in a mammalian cell-virus system.
The combined effect of 8-methoxypsoralen (8-MOP) and ultraviolet (UV) radiation on the ability of an irradiated mammalian cell (CV-1) to reactivate UV-irradiated mammalian virus (Herpes simplex) was tested. Prior treatment of cells with 8-MOP was found to increase Radiation-Enhanced Reactivation (RER) at one wavelength (297 nm) in the far ultraviolet but not at others (240-289 nm). This same treatment induced RER in the near UV (302-370 nm) and the visible region (380-400 nm). An action spectrum for the photo-sensitized induction of this cellular parameter was obtained. This action spectrum is consistent with the absorption spectrum for 8-MOP and the theory that damage to DNA is, at least in part, responsible for Radiation-Enhanced Reactivation.
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D000882:Haplorhini; D007668:Kidney; D008730:Methoxsalen / Q000494:pharmacology*; D018139:Simplexvirus / Q000254:growth & development; D014466:Ultraviolet Rays; D010948:Viral Plaque Assay; D014775:Virus Activation / Q000187:drug effects* / Q000528:radiation effects*
D008730:Methoxsalen