title: Studies of 3-aminopyridine adenine dinucleotide phosphate.
abstract: 3-Aminopyridine adenine dinucleotide phosphate (AADP) was prepared from NADP and 3-amino-pyridine through the pig brain NADase-catalyzed pyridine base exchange reaction. The purified dinucleotide was chemically characterized and spectral properties of the compound were determined. The importance of the application of AADP in studies of NADP-requiring biochemical processes was indicated by the demonstration of AADP as an effective inhibitor of five NADP-requiring enzymes, by the demonstration of the fluorescence enhancement on the binding of AADP to yeast glucose-6-phosphate dehydrogenase when glucose-6-phosphate is present, and by the functioning of AADP as a fluorimetric substrate for snake venom nucleotide pyrophosphatase.
mesh_terms: D000429:Alcohol Oxidoreductases / Q000378:metabolism; D000588:Amines; D000818:Animals; D002417:Cattle; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005969:Glutamate Dehydrogenase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007521:Isocitrate Dehydrogenase / Q000378:metabolism; D007700:Kinetics; D009243:NAD; D009249:NADP / Q000031:analogs & derivatives*; D010734:Phosphogluconate Dehydrogenase / Q000378:metabolism; D011755:Pyrophosphatases / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000588:Amines; D009243:NAD; D009249:NADP; D000429:Alcohol Oxidoreductases; D007521:Isocitrate Dehydrogenase; D010734:Phosphogluconate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D005969:Glutamate Dehydrogenase; D005980:Glutathione Reductase; D011755:Pyrophosphatases