Guinea pig cell-mediated tumor immunity: the chromium release assay detects both cytolysis and serum blocking for syngeneic chemically-induced tumors.
The chromium release test (CRT) was used to assess cell-mediated immunity to syngeneic, chemically-induced tumors in guinea pigs. The animal models were Sewall Wright strain 13 guinea pigs with 3-methyl-cholanthrene (MCA)-induced fibrosarcomas and strain 2 guinea pigs with diethylnitrosamine (DEN)-induced hepatocarcinomas. Regional lymph node cells were significantly cytolytic for the immunizing tumors, specifically so for three of the four tumors, and tumor-bearer sera could significantly block cytolysis. The two DEN-induced strain 2 hepatomas, line 1 and line 10, are antigenically distinct by the CRT but the two MCA-induced tumors have tumor specific antigens as well as a common, cross-reactive antigen.
D000818:Animals; D001667:Binding, Competitive; D006528:Carcinoma, Hepatocellular / Q000139:chemically induced*; D002471:Cell Transformation, Neoplastic; D002860:Chromium Radioisotopes; D003602:Cytotoxicity, Immunologic; D004052:Diethylnitrosamine / Q000494:pharmacology; D005354:Fibrosarcoma / Q000139:chemically induced*; D006168:Guinea Pigs; D007111:Immunity, Cellular; D008113:Liver Neoplasms / Q000139:chemically induced*; D008198:Lymph Nodes / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology; D008748:Methylcholanthrene / Q000494:pharmacology
D002860:Chromium Radioisotopes; D004052:Diethylnitrosamine; D008748:Methylcholanthrene