title: Physical maps of bovine papillomavirus type 1 and type 2 genomes.
abstract: Physical maps of bovine papillomavirus type 1 and type 2 (BPV-1 and BPV-2) DNA were constructed from analysis of the electrophoretic mobilities of restriction endonuclease cleavage fragments from dual digests. BPV-1 DNA was sensitive to Hind III, HindIII, EcoRI, HpaI, AND BamHI, with all but HindII yielding single scissions. BPV-2 DNA was resistant to EcoRI, and HindIII had one cleavage site whereas HpaI, BamHI, and HindII yielded multiple fragments. Of four BPV-1 isolates examined, DNA from one isolate was resistant to HindIII, and another DNA isolate was resistant to BamHI. The three BPV-2 isolates examined were uniformly sensitive to the restriction endonucleases employed.
mesh_terms: D052688:Bovine papillomavirus 1 / Q000032:analysis* / Q000235:genetics; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004279:DNA, Viral / Q000032:analysis* / Q000378:metabolism; D005814:Genes, Viral; D008970:Molecular Weight; D027383:Papillomaviridae / Q000032:analysis*
chemical_list: D004279:DNA, Viral; D004262:DNA Restriction Enzymes