title: Application of Raman spectroscopy to biological macromolecules.
abstract: The Raman spectra of biological macromolecules arise from molecular vibrations of either the backbone chains or the side chains. The frequencies of the Raman bands lie in a region between 200 cm-1 and 3000 cm-1. From certain frequencies of the vibrations of the backbone chains one can determine the conformation or secondary structure of a macromolecule. Thus for polypeptides and proteins the frequencies of the Amide I and Amide III vibrations allow one to determine the averge conformation of their backbone chain. In polynucleotides and nucleic acids, the frequency of the phosphate diester stretch of the phosphate furanose chain varies between 814 cm-1 for A conformation and 790 cm-1 for B conformation. Raman spectra of the bases in nucleic acids can be used to determine base stacking and hydrogen bonding interactions. Thus Raman spectroscopy is an important tool for determining the conformation structure of proteins and nucleic acids.
mesh_terms: D000818:Animals; D004247:DNA; D003903:Deuterium; D006863:Hydrogen-Ion Concentration; D008239:Lysine; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D010455:Peptides; D011063:Poly A-U; D011070:Poly I-C; D011072:Poly U; D011487:Protein Conformation; D012343:RNA, Transfer; D012542:Scattering, Radiation; D013057:Spectrum Analysis / Q000379:methods*; D013329:Structure-Activity Relationship; D013696:Temperature; D014732:Vibration; D014867:Water
chemical_list: D010455:Peptides; D014867:Water; D011063:Poly A-U; D011072:Poly U; D004247:DNA; D012343:RNA, Transfer; D003903:Deuterium; D008239:Lysine; D011070:Poly I-C