Comparative studies on the human glutamate-pyruvate transaminase phenotypes--GPT 1, GPT 2-1, GPT 2.
The quantitative differences between the activity of the 3 common phenotypes of human red cell GPT has been confirmed. In addition, the activity of red cell GPT 1 was found to be greater in young children than in adults. No such difference was found for the GPT 2 phenotype. The activity of the red cell GPT 1 was found to decrease with age, reaching the adult level at the age of 10 to 12 years. Red cell GPT of all the 3 common phenotypes in both adults and children was found to show a similar response to the addition of excess pyridoxal phosphate. A method has been devised for the partial purification of human GPT (cytoplasmic) from liver. GPT 1 and GPT 2 have been purified, and very few significant differences were found amongst the physical and kinetic parameters tested.
D000328:Adult; D000367:Age Factors; D000410:Alanine Transaminase / Q000037:antagonists & inhibitors / Q000097:blood*; D000483:Alleles; D000625:Aminooxyacetic Acid / Q000494:pharmacology; D002648:Child; D004355:Drug Stability; D004912:Erythrocytes / Q000201:enzymology*; D014644:Genetic Variation; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007223:Infant; D007527:Isoenzymes / Q000097:blood*; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D008298:Maleates / Q000494:pharmacology; D008967:Molecular Biology; D008970:Molecular Weight; D010334:Paternity; D010641:Phenotype; D011732:Pyridoxal Phosphate; D014508:Urea / Q000494:pharmacology
D007527:Isoenzymes; D008298:Maleates; D000625:Aminooxyacetic Acid; D011732:Pyridoxal Phosphate; D014508:Urea; D000410:Alanine Transaminase