Effect of serum-antigen incubation times on the expression of non-specific inhibitors of rubella hemagglutination.
Under certain conditions, serum very low-density lipoproteins (VLDL) and high-density lipoproteins (HDL) can inhibit rubella hemagglutination. The level of this non-specific hemagglutination-inhibition (HI) activity increases as the incubation period between serum and antigen is increased. Treatment of serum with heparin-MnCl2 does not precipitate HDL, and may not effect complete removal of all VLDL. This treatment method, therefore, should be considered a source of false-positive reactions, especially when extended serum-antigen incubation periods are used to enhance HI activity and to detect low levels of IgM.
D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral; D001769:Blood; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL; D012412:Rubella virus / Q000276:immunology*; D013997:Time Factors
D000914:Antibodies, Viral; D000956:Antigens, Viral; D008075:Lipoproteins, HDL; D008077:Lipoproteins, LDL; D008079:Lipoproteins, VLDL