Purification, properties and glycoprotein nature of arylsulfatase A from sheep brain.
A simple and rapid method for the purification of arylsulfatase A (EC 3.1.6.1) from sheep brain has been developed. This includes the concanavalin A-Sepharose affinity chromatography and the pH-dependent polymerization and depolymerization of the enzyme. By these methods a homogeneous enzyme was obtained and the enzyme was purified 7180-fold. Sheep brain arylsulfatase A has been shown to be a glycoprotein containing 25% neutral sugar and 0.5% sialic acid. The constituent neutral sugars were identified as glucose and mannose.
D000818:Animals; D001192:Arylsulfatases / Q000032:analysis; D001921:Brain / Q000201:enzymology*; D002553:Cerebroside-Sulfatase / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D003208:Concanavalin A; D006023:Glycoproteins / Q000032:analysis / Q000302:isolation & purification*; D006601:Hexoses / Q000032:analysis; D008970:Molecular Weight; D012756:Sheep; D012794:Sialic Acids / Q000032:analysis; D013429:Sulfatases / Q000302:isolation & purification*
D006023:Glycoproteins; D006601:Hexoses; D012794:Sialic Acids; D003208:Concanavalin A; D013429:Sulfatases; D001192:Arylsulfatases; D002553:Cerebroside-Sulfatase