Selective release of a deoxyribonucleic acid-binding factor from the surface of competent pneumococci.
Methods are described that resulted in the selective release of deoxyribonucleic acid (DNA)-binding factor from the surface of competent pneumococci. The same methods caused a parallel inactivation of the DNA-binding capacity of the extracted bacteria. Genetically or physiologically incompetent pneumococci did not yield binding factor upon exposure to the same methods. The solubilized binding factor appeared to be a protein; it could be assayed by a membrane filter binding procedure. The binding factor had properties reminiscent of those of the DNA receptors of transformable pneumococci (Seto et al., 1975).
D001426:Bacterial Proteins / Q000302:isolation & purification* / Q000378:metabolism; D002352:Carrier Proteins / Q000302:isolation & purification* / Q000378:metabolism; D002473:Cell Wall / Q000032:analysis; D004269:DNA, Bacterial / Q000378:metabolism; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008851:Micropore Filters; D009995:Osmosis; D012965:Sodium Chloride / Q000494:pharmacology; D013010:Sonication; D013296:Streptococcus pneumoniae / Q000032:analysis* / Q000378:metabolism; D013501:Surface-Active Agents / Q000494:pharmacology; D014170:Transformation, Genetic
D001426:Bacterial Proteins; D002352:Carrier Proteins; D004269:DNA, Bacterial; D013501:Surface-Active Agents; D012965:Sodium Chloride