Study of cell surface antigens induced by herpes simplex virus. Note I. Identification of the antigens by means of protein A-containing staphylococci.
A rapid, easy-to-perform and specific technique for the visualization of herpes simplex virus (HSV)-induced cell surface antigens is described. The technique consists in the incubation of HSV-infected cells with hyperimmune HSV antiserum and formation of rosettes with FITC-labelled Cowan strain staphylococci containing protein A. The "nonspecific background" of the reaction can be estimated by comparison with the rate of bacteria binding by control cells not incubated with HSV antiserum.
D000954:Antigens, Surface / Q000032:analysis*; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells; D006801:Humans; D012397:Rosette Formation; D018139:Simplexvirus / Q000276:immunology*; D013205:Staphylococcal Protein A / Q000276:immunology*
D000954:Antigens, Surface; D013205:Staphylococcal Protein A