Spontaneous production of a C-type RNA virus in a cell line derived from rat glioma.
The spontaneous production of a rat C-type RNA virus (ACV) in a cultured cell line (AC cells) established from a chemically induced rat glioma was studied. The characteristics of ACV were: morphology typical of C-type RNA virus; buoyant density of 1.15 g/ml in a sucrose density gradient; RNA directed DNA polymerase activity; viral core with a density of 1.28 to 1.30 g/ml; 70S RNA with dimer structure; and structural protein composed of mainly four polypeptides. Kinetical analysis of DNA-DNA hybridization revealed that DNA sequences homologous to DNA transcripts of RNA of ACV were present in rat cells. RNA directed DNA polymerase of ACV partially cross-reacted with antiserum to the polymerase of Rauscher murine leukemia virus. These data suggest that ACV is an endogenous C-type RNA virus of rat origin.
D000818:Animals; D002460:Cell Line; D002499:Centrifugation, Density Gradient; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D005910:Glioma / Q000382:microbiology*; D006824:Hybridization, Genetic; D008970:Molecular Weight; D012328:RNA Viruses / Q000254:growth & development* / Q000648:ultrastructure; D051381:Rats; D012190:Retroviridae / Q000254:growth & development* / Q000648:ultrastructure; D013997:Time Factors; D014529:Uridine / Q000378:metabolism
D004259:DNA-Directed DNA Polymerase; D014529:Uridine