Reactivity of neutralizing antibodies with different specificities to H particles of poliovirus.
In order to elucidate the antigenic structure of poliovirus, the reactivity of antibody produced with H antigenic particles of Mahoney strain (polio type 1) was investigated. Injection of H particles of Mahoney strain into rabbits yielded neutralizing antibody as well as CF-N and CF-H antibodies. This result coincided with the report by Hinuma and coworkers. Neutralization tests with inhibitor resistant Mahoney mutants revealed that the neutralizing antibody produced with H particles was of HN31 type, one of the five different kinds of polio neutralizing antibodies reported previously (14). Absorption experiments with H particles on different neutralizing antibodies and analysis of antibody eluted by acid dissociation from antiserum-treated H particles also showed that the HN31 type antibody specifically combined with H particles of Mahoney strain. Since the H particle of poliovirus is known to be deficient in VP4, these results seems to indicate that the HN31 type antibody reacts with a structural part(s) of poliovirus other than VP4.
D000042:Absorption; D000818:Animals; D000914:Antibodies, Viral; D000918:Antibody Specificity; D000936:Antigen-Antibody Complex; D000956:Antigens, Viral / Q000276:immunology; D001666:Binding Sites, Antibody; D002213:Capsid / Q000276:immunology; D000882:Haplorhini; D006736:Horses; D007158:Immunologic Techniques; D009500:Neutralization Tests; D017955:Poliovirus / Q000276:immunology*; D011817:Rabbits
D000914:Antibodies, Viral; D000936:Antigen-Antibody Complex; D000956:Antigens, Viral