Effects of prostaglandins on the action of luteinizing hormone in dispersed rat intestitial cells.
Prostaglandins E1, E2, A1 and A2 at 10(-5) and 10(-4)M stimulated basal testosterone production in dispersed rat interstitial cells in vitro. They effectively inhibited steroidogenesis induced by ovine pituitary luteinizing hormone (LH) (0.2 nM), dibutyryl cyclic AMP-(DBC 0-5 mM), and cholera toxin (100 ng). PGF2 alpha (10(-3) to 10(-12)M) had no effect on either basal or hormonal or non-hormonal stimulated steroidogenesis in the cells. PGA1 and PGA2 were more effective inhibitors than PGE1 and E2. None of the PG's had any influence on 125I LH-receptor interaction. In view of this and the inhibition of DBC stimulated testosterone production, it may be suggested that the PG inhibition lies beyond the receptor-cyclic AMP formation step.
D000818:Animals; D003994:Bucladesine / Q000037:antagonists & inhibitors / Q000494:pharmacology; D002772:Cholera Toxin / Q000037:antagonists & inhibitors / Q000494:pharmacology; D005260:Female; D007985:Leydig Cells / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D008297:Male; D011453:Prostaglandins / Q000494:pharmacology*; D051381:Rats; D013256:Steroids / Q000096:biosynthesis; D013739:Testosterone / Q000096:biosynthesis
D011453:Prostaglandins; D013256:Steroids; D013739:Testosterone; D003994:Bucladesine; D007986:Luteinizing Hormone; D002772:Cholera Toxin