Liquid chromatographic analysis of disopyramide and its mono-N-dealkylated metabolite.
We describe a rapid, sensitive, and specific "high performance" liquid chromatographic analysis for disopyramide and its mono-N-dealkylated metabolite in serum, urine, and saliva. We used a mu-Bondapak CN column and an acetate buffer mobile phase containing methanol. Retention times for the two compounds and the internal standard, p-chlorodisopyramide, were 3.4, 4.1, and 6.3 min, respectively. The lower limits of sensitivity for drug and metabolite were 50 and 80 micrograms/L, respectively, with maximum coefficients of variation of 4.6 and 12%, respectively. Currently used antiarrhythmic drugs did not interfere with the analysis of disopyramide, and the pharmacokinetics of the drug, obtained from studies of one subject, agree well with reported values.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004206:Disopyramide / Q000031:analogs & derivatives / Q000032:analysis* / Q000097:blood / Q000652:urine; D006801:Humans; D007700:Kinetics; D011725:Pyridines / Q000032:analysis*; D012463:Saliva / Q000032:analysis
D011725:Pyridines; C030438:4-(isopropylamino)-2-(2-pyridyl)-2-phenylbutyramide; D004206:Disopyramide