title: Light-scattering effects in the measurement of membrane microviscosity with diphenylhexatriene.
abstract: Data from several membrane systems are presented to confirm an empirical means of correcting diphenylhexatriene fluorescence for depolarization caused by sample turbidity. The depolarization proportionally constants obtained are not equal, but are shown to vary with (a) the physical state of the membrane, (b) the cholesterol content of the membrane, (c) the protein content of the membrane, and (d) the method of membrane preparation or isolation. It is concluded that depolarization corrections must always be considered when diphenylhexatriene fluorescence anisotropy is used to compare the fluidities within different membrane bilayers.
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000502:physiology; D002642:Chick Embryo; D002784:Cholesterol; D004161:Diphenylhexatriene / Q000494:pharmacology*; D005454:Fluorescence Polarization; D007425:Intracellular Membranes / Q000502:physiology; D008027:Light; D008081:Liposomes; D008566:Membranes / Q000187:drug effects / Q000502:physiology*; D010713:Phosphatidylcholines; D011090:Polyenes / Q000494:pharmacology*; D012160:Retina / Q000502:physiology; D012519:Sarcoplasmic Reticulum / Q000502:physiology; D012542:Scattering, Radiation; D014783:Viscosity
chemical_list: D008081:Liposomes; D010713:Phosphatidylcholines; D011090:Polyenes; D004161:Diphenylhexatriene; D002784:Cholesterol
references.citation: Biochemistry. 1976 Oct 5;15(20):4521-8
references.pmid: 974073
references.citation: Biochemistry. 1977 May 31;16(11):2319-24
references.pmid: 577184
references.citation: Biochemistry. 1978 Nov 28;17(24):5248-55
references.pmid: 153147
references.citation: Biochemistry. 1978 Oct 17;17(21):4475-80
references.pmid: 581473
references.citation: Biochemistry. 1978 Jan 24;17(2):327-31
references.pmid: 619993
references.citation: Biochim Biophys Acta. 1976 Nov 2;448(4):620-4
references.pmid: 974150
references.citation: J Biol Chem. 1974 Apr 25;249(8):2652-7
references.pmid: 4822508
references.citation: J Mol Biol. 1974 Jan 5;85(4):603-15
references.pmid: 4851371
references.citation: Biochemistry. 1971 May 25;10(11):2106-13
references.pmid: 4104937
references.citation: Biochim Biophys Acta. 1972 Oct 23;288(1):231-6
references.pmid: 4118280
references.citation: Biochemistry. 1973 Jan 30;12(3):521-8
references.pmid: 4683495
references.citation: Biochim Biophys Acta. 1976 Jun 4;436(1):101-12
references.pmid: 945073
references.citation: Photochem Photobiol. 1969 Dec;10(6):363-74
references.pmid: 5372038