title: Diadenosine 5',5'''-P1,P4-tetraphosphate triggers initiation of in vitro DNA replication in baby hamster kidney cells.
abstract: Addition of diadenosine 5',5'''-P1,P4-tetraphosphate (Ap4A) to permeabilized G1-arrested baby hamster kidney cells resulted in the stimulation of DNA synthesis. No stimulation was observed in cells from exponentially growing cultures. The Ap4A-stimulated [3H]dTTP incorporation was inhibited by nalidixic acid, daunomycin, chloroquine diphosphate, EDTA, and N-ethylmaleimide. It was dose-dependent in regard to the amount of permeabilized cells and of Ap4A. Numerous replication eyes were formed in the DNA molecules of stimulated cells. Pulse-chase experiments showed that the synthesis of DNA was discontinuous, resulting in the appearance of approximately 4S Okazaki fragments and their ligation to high molecular weight DNA. These results strongly suggest that Ap4A stimulated the initiation of DNA synthesis in baby hamster kidney cells that had been arrested in G1 by serum deprival.
mesh_terms: D000227:Adenine Nucleotides / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D004261:DNA Replication / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D008854:Microscopy, Electron; D009843:Oligoribonucleotides / Q000494:pharmacology; D013329:Structure-Activity Relationship
chemical_list: D000227:Adenine Nucleotides; D009843:Oligoribonucleotides
references.citation: Biochim Biophys Acta. 1977 Mar 18;475(2):254-66
references.pmid: 843529
references.citation: Eur J Biochem. 1977 Jun 1;76(1):7-12
references.pmid: 880950
references.citation: Biochem Biophys Res Commun. 1977 Mar 21;75(2):414-9
references.pmid: 851447
references.citation: Proc Natl Acad Sci U S A. 1976 Nov;73(11):3933-6
references.pmid: 792878
references.citation: Proc Natl Acad Sci U S A. 1976 Nov;73(11):3984-8
references.pmid: 1069282
references.citation: Biochem Biophys Res Commun. 1968 Jan 25;30(2):166-72
references.pmid: 5641865
references.citation: Nat New Biol. 1972 Sep 20;239(90):74-6
references.pmid: 4512827
references.citation: J Mol Biol. 1972 Dec 30;72(3):523-37
references.pmid: 4349757
references.citation: Biochem Biophys Res Commun. 1966 Jul 6;24(1):98-105
references.pmid: 4290754
references.citation: Cell. 1976 Mar;7(3):447-53
references.pmid: 947552
references.citation: Cell. 1976 Mar;7(3):439-45
references.pmid: 947551
references.citation: J Virol. 1975 Feb;15(2):259-67
references.pmid: 163354
references.citation: Proc Natl Acad Sci U S A. 1975 Jun;72(6):2437-41
references.pmid: 1056038
references.citation: Chromosoma. 1973 Jul 23;43(1):1-18
references.pmid: 4199811
references.citation: Proc Natl Acad Sci U S A. 1974 Jan;71(1):135-9
references.pmid: 4204203
references.citation: Nature. 1974 Oct 18;251(5476):637-9
references.pmid: 4607806
references.citation: Eur J Biochem. 1974 Dec 2;49(3):565-71
references.pmid: 4474940
references.citation: J Biol Chem. 1975 Jun 10;250(11):4348-54
references.pmid: 165198
references.citation: J Virol. 1975 Jan;15(1):97-107
references.pmid: 163350
references.citation: Biochim Biophys Acta. 1972 Dec 22;287(3):415-26
references.pmid: 4641252
references.citation: Proc Natl Acad Sci U S A. 1976 Jul;73(7):2231-5
references.pmid: 1065872
references.citation: Eur J Biochem. 1976 Apr 15;64(1):307-12
references.pmid: 1278158
references.citation: J Mol Biol. 1975 Dec 5;99(2):317-37
references.pmid: 1206707
references.citation: J Mol Biol. 1970 Jul 14;51(1):61-73
references.pmid: 5481280
references.citation: Nature. 1970 Jan 10;225(5228):159-64
references.pmid: 5409962
references.citation: J Cell Sci. 1969 Nov;5(3):625-43
references.pmid: 5361237
references.citation: Biochem Biophys Res Commun. 1969 Aug 22;36(5):756-63
references.pmid: 5808290
references.citation: Biochim Biophys Acta. 1968 Jul 23;161(2):455-68
references.pmid: 5667292
references.citation: J Org Chem. 1965 Oct;30(10):3381-7
references.pmid: 5846146