Diphtheria toxin has the properties of a lectin.
The inhibition of protein synthesis in Chinese hamster V79 cells by diphtheria toxin is antagonized by the lectins concanavalin A, succinylated concanavalin A, and wheat germ agglutinin but not by Proteus vulgaris phytohemagglutinin or abrus agglutinin. The effects of concanavalin A and wheat germ agglutinin are reversed by methyl alpha-mannoside and N-acetylglucosamine, respectively. The inhibition of diphtheria toxin as a function of concanavalin A concentration fits a model of competitive inhibition with an apparent dissociation constant for concanavalin A of 3 X 10(-8) M. These results suggest that the diphtheria toxin receptor may be an oligosaccharide. To test this hypothesis, we screened several oligosaccharides for the ability to inhibit diphtheria toxin. The cell wall polysaccharide of Salmonella cholera suis and the ovalbumin glycopeptide were effective inhibitors. These studies suggest that diphtheria toxin may have the oligosaccharide binding properties of a lectin with specificity for N-acetylglucosamine and mannose.
D002460:Cell Line; D003208:Concanavalin A / Q000494:pharmacology; D004166:Diphtheria Antitoxin; D037102:Lectins / Q000494:pharmacology*; D009844:Oligosaccharides / Q000494:pharmacology; D037121:Plant Lectins; D011135:Polysaccharides, Bacterial / Q000494:pharmacology; D014176:Protein Biosynthesis; D011955:Receptors, Drug / Q000187:drug effects*; D013329:Structure-Activity Relationship; D014908:Triticum
D004166:Diphtheria Antitoxin; D037102:Lectins; D009844:Oligosaccharides; D037121:Plant Lectins; D011135:Polysaccharides, Bacterial; D011955:Receptors, Drug; D003208:Concanavalin A
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