Regulation of IgE antibody production by serum molecules. VI: Preliminary biochemical and immunological characterization of serum molecules active in suppressing IgE antibody production.
Molecules present in the serum and ascites fluids of low IgE responder mice previously inoculated with complete Freund's adjuvant have been analyzed in terms of certain biochemical and immunological characteristics. These studies demonstrate that the active molecules, termed "suppressive factors of allergy" (SFA), are (1) nondialyzable, (2) not associated with low-density or high-density lipoproteins, (3) heat stable, (4) precipitable by ammonium sulfate, and (5) approximately 150,000 daltons in molecular size. Studies with immunoadsorbents prepared from various antisera indicate that the suppressive molecules are (1) not immunoglobulin in nature, (2) not reactive with specific anti-H-2 alloantibodies, but (3) reactive with anti-beta 2m antibodies as well as (4) heterologous antisera raised against CFA-immune mouse serum.
D000818:Animals; D000937:Antigen-Antibody Reactions; D001202:Ascitic Fluid / Q000276:immunology*; D001798:Blood Proteins / Q000032:analysis / Q000276:immunology* / Q000494:pharmacology; D003864:Depression, Chemical; D007073:Immunoglobulin E / Q000096:biosynthesis*; D007166:Immunosuppressive Agents; D051379:Mice; D008970:Molecular Weight; D011817:Rabbits
D001798:Blood Proteins; D007166:Immunosuppressive Agents; D007073:Immunoglobulin E