title: Amino acid sequence of the beta chain of human fibrinogen: homology with the gamma chain.
abstract: The beta chain of human fibrinogen is composed of 452 +/- 5 amino acid residues, 14 of which are methionines. Consistent with these findings we have isolated and characterized 15 fragments after cyanogen bromide digestion of carboxymethylated beta chains. The arrangement of several of these peptides was deduced on the basis of overlapping peptides isolated from the fragments D and E produced by the plasmic digestion of fibrinogen and/or from a tryptic digest of citraconylated beta chains. Most of the other cyanogen bromide fragments can be aligned by homology with the alpha and/or gamma chains from human fibrinogen, although the positioning of a few of the smallest peptides is still ambiguous. The homology of the beta chain with the gamma chain is especially strong in certain regions of the domain that includes fragment D.
mesh_terms: D000595:Amino Acid Sequence; D005340:Fibrinogen; D006801:Humans; D046911:Macromolecular Substances; D010446:Peptide Fragments
chemical_list: D046911:Macromolecular Substances; D010446:Peptide Fragments; D005340:Fibrinogen
references.citation: Ann Inst Pasteur (Paris). 1961 Mar;100:377-89
references.pmid: 13729934
references.citation: Biochemistry. 1977 Apr 19;16(8):1710-5
references.pmid: 139916
references.citation: Hoppe Seylers Z Physiol Chem. 1976 Dec;357(12):1801-3
references.pmid: 138657
references.citation: Hoppe Seylers Z Physiol Chem. 1977 Dec;358(12):1643-6
references.pmid: 590945
references.citation: Mol Biol Biochem Biophys. 1977;25:1-20
references.pmid: 917005
references.citation: Hoppe Seylers Z Physiol Chem. 1977 Jul;358(7):935-8
references.pmid: 892718
references.citation: Biochemistry. 1977 Apr 19;16(8):1703-9
references.pmid: 856257
references.citation: Anal Biochem. 1977 Apr;78(2):491-505
references.pmid: 851221
references.citation: Hoppe Seylers Z Physiol Chem. 1976 Nov;357(11):1509-13
references.pmid: 1002128
references.citation: Ann N Y Acad Sci. 1972 Dec 8;202:77-97
references.pmid: 4118185
references.citation: Thromb Res. 1975 Nov;7(5):813-8
references.pmid: 54947
references.citation: Adv Protein Chem. 1973;27:1-109
references.pmid: 4589664
references.citation: Proc Natl Acad Sci U S A. 1972 Dec;69(12):3506-9
references.pmid: 4509307
references.citation: Biochim Biophys Acta. 1975 Apr 29;386(2):617-22
references.pmid: 124585
references.citation: J Biol Chem. 1975 Aug 10;250(15):5808-17
references.pmid: 125279
references.citation: Biochim Biophys Acta. 1972 Apr 15;263(2):351-67
references.pmid: 5031163
references.citation: Fed Proc. 1976 Aug;35(10):2145-9
references.pmid: 947794
references.citation: Thromb Res. 1976 May;8(5):639-58
references.pmid: 936108
references.citation: Biochim Biophys Acta. 1971 Jun 29;236(3):550-62
references.pmid: 5559962
references.citation: Arch Biochem Biophys. 1966 Sep 26;116(1):271-9
references.pmid: 5961838