Antibodies to tubulin in normal nonimmunized animals.
Sera of normal nonimmunized rabbits, pigs, calves, and humans contain tubulin-reactive antibodies. Usually, low amounts of antibodies against tubulin of the IgG class (2.5-4 mg/100 ml of serum from nonimmunized animals) were isolated. Anti-tubulin antibodies were also produced by injecting pig tubulin in complete Freund's adjuvant into rabbits. Slightly higher amounts of anti-tubulin antibody were isolated from sera of immunized rabbits (7 mg/100 ml of serum). The cytoplasmic network of microtubules of Tcc 36 mouse cells in culture was not clearly stained by natural anti-tubulin antibodies, but dense staining of the centrosphere was observed. In contrast, induced anti-tubulin antibodies densely stained cytoplasmic microtubular networks. Vinblastine-induced tubulin paracrystals were equally stained by natural and induced anti-tubulin antibodies.
D000818:Animals; D000831:Animals, Newborn; D000906:Antibodies / Q000302:isolation & purification; D002417:Cattle / Q000276:immunology; D002478:Cells, Cultured; D005856:Germ-Free Life; D006023:Glycoproteins / Q000276:immunology*; D006801:Humans; D007122:Immunoelectrophoresis; D009113:Muramidase / Q000276:immunology; D011817:Rabbits / Q000276:immunology; D013045:Species Specificity; D013552:Swine / Q000276:immunology; D014404:Tubulin / Q000276:immunology*; D014747:Vinblastine / Q000494:pharmacology
D000906:Antibodies; D006023:Glycoproteins; D014404:Tubulin; D014747:Vinblastine; D009113:Muramidase
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