Control of antibody heterogeneity in strain 2 guinea pigs.
When Wright's strain 2 guinea pigs are immunized with 2,4-dinitrophenyl conjugates in complete Freund's adjuvant, the antibody response varies with the choice of carrier. Immunization with 2,4-dinitrophenyl-guinea pig albumin elicits a response that requires approximately 21 days to detect. The antibody produced is, according to isoelectric focusing, relatively homogeneous IgG2 having a neutral isoelectric point. On small amounts of IgG are produced. Stimulation of animals with 2,4-dinitrophenyl-keyhole Limpet hemocyanin produces a response by 14 days is similar to the peak response inititated by 2,4-dinitrophenyl-guinea pig albumin. With time, however, basic IgG2 populations are added to the response. By days 28-35, when the anti-hapten response has reached a plateau, the major subpopulation of antibody is neutral IgG2, but there exists several times as much basic IgG2 as IgG1. These data suggest that antigen-responsive cells may be ordered into groups having different thresholds of activation. Regardless of the strength of the antigenic stimulus, there is a set sequence of activation. The same cells always play the primary role, contributing most of the antibody regardless of whether secondary clones of cells are activated.
D000818:Animals; D000917:Antibody Formation; D004136:Dinitrobenzenes / Q000276:immunology; D005796:Genes; D006168:Guinea Pigs; D006433:Hemocyanins / Q000276:immunology; D007074:Immunoglobulin G / Q000096:biosynthesis; D007518:Isoantibodies / Q000235:genetics*; D007525:Isoelectric Focusing; D012709:Serum Albumin / Q000276:immunology; D013997:Time Factors
D004136:Dinitrobenzenes; D007074:Immunoglobulin G; D007518:Isoantibodies; D012709:Serum Albumin; D006433:Hemocyanins
J Immunol. 1965 Jun;94:966-75
14321441
J Exp Med. 1963 Dec 1;118:1021-35
14112263
Nature. 1962 May 5;194:495-6
14450081
Cell Immunol. 1977 Oct;33(2):245-56
912760
Int Arch Allergy Appl Immunol. 1976;51(4):416-28
965103
J Immunol. 1974 Aug;113(2):677-87
4136042
Nature. 1968 Jul 6;219(5149):66-7
4173351
Immunology. 1965 Sep;9(3):205-17
4158286
Immunology. 1966 Feb;10(2):179-86
4160441
Adv Immunol. 1974;18:1-66
4275202
J Immunol. 1972 Sep;109(3):457-63
5066344
J Immunol. 1976 May;116(5):1400-6
774983
J Immunol. 1975 Feb;114(2 pt 2):863-71
1112982
Anal Biochem. 1971 Jan;39(1):93-112
5544614
J Biol Chem. 1970 Jun;245(12):3059-65
5432796