The role of macrophages in stimulation of immune induction and myelopoiesis. I: Comparison of activity of macrophage-derived factors in granulopoiesis and immunostimulation.
Hemopoietic growth and immunological inductive factors from human peripheral leukocyte conditioned medium and mouse macrophage culture supernatant fluids were compared. Factors derived from human peripheral leukocyte conditioned medium were found to substitute for those of mouse macrophage derivation in immunological assays, namely, induction of a cytotoxic T-lymphocyte response in macrophage-depleted mouse spleen cultures. Association of macrophage-derived factors with B2-microglobulin (B2m) antigen determinants was observed by inhibition with great antihuman B2m, affinity chromatography, and direct replacement of mouse macrophage-derived factors with human urinary B2m.
D000818:Animals; D000917:Antibody Formation; D002454:Cell Differentiation; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic; D006098:Granulocytes / Q000166:cytology; D006410:Hematopoiesis; D006801:Humans; D008222:Lymphokines / Q000494:pharmacology*; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H / Q000276:immunology
D003470:Culture Media; D008222:Lymphokines