title: Low-molecular-weight peptide inhibits RNA synthesis in human leukemic and phytohemagglutinin-stimulated leukocytes and globin mRNA transcription in differentiating Friend cells.
abstract: The RNA synthesis of human leukemic leukocytes and phytohemagglutinin-stimulated lymphocytes is markedly reduced by administration of a low-molecular-weight nonhistone peptide factor from calf thymus. Treatment with the factor strongly inhibits hemoglobin production and globin mRNA transcription in dimethyl sulfoxide-stimulated Friend cells without appreciably modifying the rate of cell growth. Evidence for specificity of these effects is provided by the lack of action of the factor on both growth rate and RNA synthesis of a number of nondifferentiating cell lines from various animal species. After removal of the compound, both human lymphocytes and Friend cells can be stimulated by phytohemagglutinin and by dimethyl sulfoxide, respectively, ruling out any toxic effect.
mesh_terms: D000818:Animals; D002417:Cattle; D002455:Cell Division / Q000187:drug effects; D005914:Globins / Q000096:biosynthesis; D006801:Humans; D007938:Leukemia / Q000097:blood; D007962:Leukocytes / Q000378:metabolism*; D008213:Lymphocyte Activation; D010455:Peptides / Q000494:pharmacology*; D012313:RNA / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000096:biosynthesis*; D012334:RNA, Neoplasm / Q000096:biosynthesis; D014158:Transcription, Genetic / Q000187:drug effects*
chemical_list: D010455:Peptides; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D012313:RNA; D005914:Globins
references.citation: Proc Natl Acad Sci U S A. 1975 Jan;72(1):98-102
references.pmid: 164031
references.citation: Proc Natl Acad Sci U S A. 1977 May;74(5):2036-40
references.pmid: 266723
references.citation: Nature. 1976 Aug 12;262(5569):622-3
references.pmid: 958432
references.citation: Mol Biol Rep. 1976 Sep;3(1):55-64
references.pmid: 1034203
references.citation: Biochim Biophys Acta. 1975 Nov 18;414(1):9-19
references.pmid: 1191703
references.citation: Cancer Res. 1973 Feb;33(2):428-34
references.pmid: 4510929
references.citation: Proc Natl Acad Sci U S A. 1973 Oct;70(10):2956-9
references.pmid: 4517948
references.citation: Physiol Rev. 1974 Apr;54(2):272-315
references.pmid: 4594651
references.citation: Ann N Y Acad Sci. 1974 Nov 29;241(0):290-309
references.pmid: 4611306
references.citation: Nature. 1967 Apr 29;214(5087):496-7
references.pmid: 4962069
references.citation: Proc Natl Acad Sci U S A. 1966 Apr;55(4):805-12
references.pmid: 5219687
references.citation: Proc Natl Acad Sci U S A. 1971 Feb;68(2):378-82
references.pmid: 5277089
references.citation: J Biol Chem. 1968 Jan 10;243(1):34-43
references.pmid: 5635946
references.citation: Blood. 1956 Apr;11(4):380-3
references.pmid: 13304127
references.citation: Proc Natl Acad Sci U S A. 1962 Aug;48(8):1439-42
references.pmid: 16590986
references.citation: Proc Natl Acad Sci U S A. 1967 Mar;57(3):821-8
references.pmid: 16591536