title: Isopeptide linkage between nonhistone and histone 2A polypeptides of chromosomal conjugate-protein A24.
abstract: Chromosomal protein A24 has a unique structure inasmuch as it contains histone 2A and a nonhistone polypeptide the sequence of which has been partially determined. Comparative analysis of the ninhydrin-insensitive amino-terminal tryptic peptides of protein A24 and histone 2A and a quantitative analysis of their carboxyl-terminal amino acid indicated that protein A24 has two amino termini and one carboxyl terminus. The amino acid sequence analysis of tryptic peptide 17' of protein A24: (see text) showed it contains tryptic peptide 17 of histone 2A, Lys-Thr-Glu-Ser-His-His-Lys. Lysine 119, the amino terminus of this peptide, which is derived from the histone 2A portion of protein A24, is linked by an isopeptide bond to the carboxyl group of a glycine residue. Accordingly, the branched structure of protein A24 proposed is: (see text).
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002868:Chromosomal Proteins, Non-Histone; D006657:Histones; D010455:Peptides / Q000032:analysis; D013950:Thymus Gland; D014357:Trypsin
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D006657:Histones; D010455:Peptides; D014357:Trypsin
references.citation: Biochim Biophys Acta. 1958 Apr;28(1):184-91
references.pmid: 13535695
references.citation: Can J Biochem Physiol. 1958 Jan;36(1):71-4
references.pmid: 13500244
references.citation: J Biol Chem. 1976 Oct 10;251(19):5901-3
references.pmid: 972147
references.citation: Annu Rev Biochem. 1975;44:725-74
references.pmid: 1094921
references.citation: Science. 1974 Jan 25;183(4122):330-2
references.pmid: 4128918
references.citation: Biochem Biophys Res Commun. 1968 Feb 26;30(4):356-62
references.pmid: 5637041
references.citation: Proc Natl Acad Sci U S A. 1975 Oct;72(10):3978-82
references.pmid: 1060078
references.citation: Science. 1976 Sep 3;193(4256):848-56
references.pmid: 948749
references.citation: Proc Natl Acad Sci U S A. 1973 May;70(5):1316-20
references.pmid: 4351171
references.citation: Biochemistry. 1975 May 20;14(10):2214-8
references.pmid: 1170880
references.citation: J Biol Chem. 1972 Oct 10;247(19):6018-23
references.pmid: 4568599
references.citation: J Biol Chem. 1972 Jun 10;247(11):3589-91
references.pmid: 4555425
references.citation: Life Sci. 1974 May 16;14(10):1835-45
references.pmid: 4847790
references.citation: Science. 1974 May 24;184(4139):868-71
references.pmid: 4825889
references.citation: Science. 1974 May 24;184(4139):865-8
references.pmid: 4825888
references.citation: Physiol Chem Phys. 1972;4(1):10-6
references.pmid: 4667695
references.citation: Biochem Biophys Res Commun. 1972 Jan 14;46(1):50-5
references.pmid: 5061679
references.citation: Science. 1968 May 24;160(3830):892-3
references.pmid: 4967475
references.citation: J Biol Chem. 1967 Apr 25;242(8):1833-7
references.pmid: 5337592
references.citation: J Biol Chem. 1975 Sep 10;250(17):6806-16
references.pmid: 808547
references.citation: Proc Natl Acad Sci U S A. 1976 May;73(5):1572-5
references.pmid: 1064026
references.citation: Biochemistry. 1976 Aug 10;15(16):3510-5
references.pmid: 952874
references.citation: Biochim Biophys Acta. 1976 Mar 18;427(1):315-24
references.pmid: 1260005
references.citation: J Biol Chem. 1975 Aug 10;250(15):5921-5
references.pmid: 1150666
references.citation: Biochem Biophys Res Commun. 1975 Aug 4;65(3):951-60
references.pmid: 1156427
references.citation: J Biol Chem. 1975 Sep 25;250(18):7182-7
references.pmid: 1165239
references.citation: Anal Biochem. 1975 May 12;65(1-2):281-92
references.pmid: 1130680
references.citation: Biochemistry. 1971 Dec 21;10(26):4912-21
references.pmid: 5134536
references.citation: J Biol Chem. 1968 Apr 25;243(8):2038-47
references.pmid: 5646494
references.citation: Biochem J. 1968 Mar;107(2):135-8
references.pmid: 5641869
references.citation: Biochemistry. 1967 Oct;6(10):3204-27
references.pmid: 6056983