Defective regulation of cholesterol biosynthesis and plasma membrane fluidity in a Chinese hamster ovary cell mutant.
A Chinese hamster ovary cell mutant resistant to killing by 25-hydroxycholesterol is shown to be defective in the regulation of cholesterol synthesis by exogenous cholesterol. When grown with various cholesterol supplements in delipidized serum, the mutant cell exhibits a variation in cholesterol content and plasma membrane fluidity that is not observed in the parental cell type.
D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology*; D002784:Cholesterol / Q000096:biosynthesis*; D008563:Membrane Lipids / Q000096:biosynthesis*; D009154:Mutation; D013113:Spin Labels
D008563:Membrane Lipids; D013113:Spin Labels; D002784:Cholesterol
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