Structural studies of synthetic filaments prepared from column-purified myosin.
Synthetic filaments prepared from column-purified rabbit skeletal myosin by slow dialysis exhibit characteristic bipolar organization and 14-nm axial subunit spacing. Backbone substructure can be discerned in high resolution micrographs in the form of striations of 3--4-nm width and slight angular tilt from the direction of the filament axis. Filament backbone diameters vary over the population, although remaining relatively constant for a single filament. Approximately 25% of the filaments appear poorly stained and frayed, which may be due to collapse on the electron microscope grid. Optical diffraction studies reveal a 43-nm axial repeat as well as the 14.3-nm subunit repeat, indicating a structural homology with natural filaments. A model for synthetic filament aggregation is presented that is consistent with observations of backbone diameter variation, absence of bare zones, and the presence of fraying filaments.
D000818:Animals; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003599:Cytoskeleton / Q000648:ultrastructure*; D008854:Microscopy, Electron; D008968:Molecular Conformation; D009218:Myosins / Q000302:isolation & purification; D011817:Rabbits
D009218:Myosins
Biochim Biophys Acta. 1954 Oct;15(2):165-73
13208680
Arch Biochem Biophys. 1953 Feb;42(2):305-20
13031633
Biochemistry. 1977 May 31;16(11):2441-9
140701
Biochemistry. 1978 Apr 4;17(7):1234-9
26379
J Mol Biol. 1977 Jun 15;113(1):199-218
881734
Philos Trans R Soc Lond B Biol Sci. 1971 May 27;261(837):201-8
4399205
J Mol Biol. 1974 Feb 15;83(1):83-97
4817801
J Mol Biol. 1972 Dec 30;72(3):619-32
4349760
J Mol Biol. 1973 Mar 15;74(4):653-76
4269687
J Mol Biol. 1974 Jan 25;82(3):421-3
4594144
Science. 1976 Oct 8;194(4261):190-2
8842
J Mol Biol. 1973 Jun 15;77(1):165-75
4769837
J Mol Biol. 1975 Dec 15;99(3):461-75
765477
J Mol Biol. 1975 Sep 5;97(1):1-9
1100851
J Cell Biol. 1975 Oct;67(1):93-104
1236853
J Mol Biol. 1975 Dec 15;99(3):445-59
1214296
J Mol Biol. 1970 Jun 14;50(2):279-95
5476917
J Mol Biol. 1967 Dec 14;30(2):383-434
5586931
J Mol Biol. 1966 Sep;20(2):391-401
5970667