Methicillin-resistant Staphylococcus aureus susceptibility testing by an automated system, Autobac I.
The Autobac I system was used to evaluate the antibiotic susceptibility pattern of methicillin-resistant Staphylococcus aureus isolates. The results of the Autobac I were compared with the results of the disk diffusion method. The disk diffusion susceptibility pattern showed resistance to methicillin/oxacillin, penicillin, erythromycin, clindamycin, and kanamycin. All isolates were susceptible to cephalothin, chloramphenicol, tetracycline, and gentamicin. There was at least 96% agreement using the Autobac I system with all antibiotics except methicillin and clindamycin. Seventy percent of 57 isolates were recorded as susceptible to methicillin, whereas 9% had an intermediate susceptibility. With clindamycin, 14% were recorded as susceptible and 7% were recorded as intermediate. Upon prolonged incubation of the Autobac I cuvette, the agreement between the two methods was 44% for methicillin and 93% for clindamycin. Changes in the environmental conditions, such as use of 5% sodium chloride broth and a 32 degrees C incubation temperature, did not increase the detection of methicillin-resistant isolates by the Autobac I system.
D001434:Bacteriophage Typing; D005069:Evaluation Studies as Topic; D008712:Methicillin / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests / Q000295:instrumentation* / Q000379:methods; D010403:Penicillin Resistance; D013211:Staphylococcus aureus / Q000187:drug effects*
D008712:Methicillin
Am J Clin Pathol. 1977 May;67(5):489-95
324263
J Med Microbiol. 1969 Nov 4;2(4):443-56
5404802
Am J Clin Pathol. 1966 Apr;45(4):493-6
5325707
N Engl J Med. 1968 Aug 29;279(9):441-8
4232865
Ann N Y Acad Sci. 1974 Jul 31;236(0):413-34
4608264
Antimicrob Agents Chemother. 1973 Sep;4(3):263-9
4586140
Lancet. 1975 Oct 4;2(7936):650-3
52016
Am J Med Sci. 1972 Sep;264(3):197-205
4486503
Appl Microbiol. 1972 Aug;24(2):240-7
5071651
Am J Med. 1976 Sep;61(3):340-5
1048860
Am J Clin Pathol. 1975 Sep;64(3):372-7
1163488
Ann Intern Med. 1967 Jul;67(1):81-9
5182354
Med J Aust. 1968 Mar 16;1(11):444-6
5185252
N Engl J Med. 1967 Sep 28;277(13):678-80
5182623
JAMA. 1970 Jul 13;213(2):257-63
5467891
Antimicrob Agents Chemother. 1975 Apr;7(4):466-80
1147582