title: In vivo analysis of cellular replication.
abstract: The number of previous cell replications that a metaphase cell has undergone in the presence of BrdUrd can be determined by the differential fluorescent patterns of metaphse chromosomes stained with Hoechst dye 33258. To examine if this technique could be applied to analyzing cell cycle kinetics in vivo, we infused Wistar rats with BrdUrd for 7.5-33 hr at concentrations of the nucleotide analog that did not inhibit cellular replication. Examination of the frequency of one, two, and three or more replication cycle cells as a function of BrdUrd infusion time indicates that cell replication times for rat bone marrow cells are relatively homogeneous. Analysis of this data with a computer simulation model produced a mean cell cycle duration of 9.2 hr, which is compatible with the fastest times obtained with radioisotope studies. These results support the potential of nonradioisotope analysis of cell replication in vivo.
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000502:physiology*; D001854:Bone Marrow Cells; D001973:Bromodeoxyuridine / Q000378:metabolism / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002875:Chromosomes / Q000378:metabolism*; D004261:DNA Replication; D004305:Dose-Response Relationship, Drug; D005260:Female; D005456:Fluorescent Dyes; D007700:Kinetics; D008938:Mitosis; D051381:Rats
chemical_list: D005456:Fluorescent Dyes; D001973:Bromodeoxyuridine
references.citation: Chromosoma. 1976 Jun 30;56(2):101-9
references.pmid: 976016
references.citation: Chromosoma. 1976 Nov 29;58(4):325-40
references.pmid: 63363
references.citation: Exp Cell Res. 1976 Oct 15;102(2):426-9
references.pmid: 976355
references.citation: Exp Cell Res. 1976 Oct 15;102(2):232-6
references.pmid: 976347
references.citation: Proc Natl Acad Sci U S A. 1973 Dec;70(12):3395-9
references.pmid: 4128545
references.citation: Science. 1974 Jul 5;185(4145):74-6
references.pmid: 4134970
references.citation: Chromosoma. 1974;48(4):341-53
references.pmid: 4141298
references.citation: Chromosoma. 1976 Aug 4;57(1):13-8
references.pmid: 60205
references.citation: Proc Natl Acad Sci U S A. 1973 Apr;70(4):1263-7
references.pmid: 4515625
references.citation: J Histochem Cytochem. 1975 Jul;23(7):493-505
references.pmid: 1095650
references.citation: Exp Cell Res. 1976 Jul;100(2):396-9
references.pmid: 945773
references.citation: Nature. 1976 Apr 1;260(5550):449-51
references.pmid: 1256591
references.citation: Nature. 1976 Apr 1;260(5550):448-9
references.pmid: 1256590
references.citation: Chromosoma. 1975 Jul 21;51(3):261-7
references.pmid: 1149577
references.citation: Cell Tissue Kinet. 1975 Mar;8(2):97-110
references.pmid: 1125970
references.citation: Nature. 1970 Jul 4;227(5253):73-5
references.pmid: 5422629
references.citation: Nature. 1967 Jun 3;214(5092):1017-8
references.pmid: 6055394
references.citation: Exp Cell Res. 1965 Sep;39(2):528-38
references.pmid: 5838695