Molecular weight and valence of the cell-surface receptor for immunoglobulin E.
The molecular weight of the active solubilized cell-surface receptor for immunoglobulin E (IgE) was measured in nonionic detergent. The diffusion coefficient was estimated by gel filtration, the partial specific volume was estimated from the differential sedimentation in sucrose gradients prepared from H2O and D2O, and the sedimentation constant was estimated from the same centrifugation experiments. The receptor has an apparent molecular weight of 130,000. Its high partial specific volume (0.81 cm3/g) suggests that bound detergent contributes significantly to the mass. The molecular weights of the receptor-IgE complex and of unbound IgE determined similarly were 310,000 and 200,000 respectively, clearly showing that the receptor is univalent. The implications of these results for the subunit structure of the receptor, receptor-membrane integration, and a possible mechanism of receptor triggering are discussed.
D000818:Animals; D001491:Basophils / Q000276:immunology; D001665:Binding Sites; D003902:Detergents; D004058:Diffusion; D006023:Glycoproteins / Q000378:metabolism; D007073:Immunoglobulin E / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism; D008961:Models, Structural; D008970:Molecular Weight; D011487:Protein Conformation; D051381:Rats; D012995:Solubility; D014461:Ultracentrifugation
D003902:Detergents; D006023:Glycoproteins; D008565:Membrane Proteins; D007073:Immunoglobulin E
J Biol Chem. 1960 May;235:1326-34
13819264
J Biol Chem. 1965 Aug;240:3325-33
14328368
J Biol Chem. 1961 May;236:1372-9
13767412
Nature. 1976 Dec 9;264(5586):548-50
826833
J Biol Chem. 1972 Sep 10;247(17):5580-6
5066408
Proc Natl Acad Sci U S A. 1970 Jul;66(3):651-6
4913206
J Immunol. 1968 Nov;101(5):905-14
4177054
Int Arch Allergy Appl Immunol. 1966;29(2):185-9
4160044
Immunochemistry. 1976 May;13(5):417-23
780265
Immunology. 1974 Apr;26(4):713-23
4212482
Proc Natl Acad Sci U S A. 1974 Oct;71(10):3913-6
4530270
J Exp Med. 1974 Mar 1;139(3):600-16
4812630
Biochem J. 1972 Feb;126(3):761-4
5075279
FEBS Lett. 1972 Jul 15;24(1):63-8
5086619
Adv Immunol. 1970;12:57-116
4921862
Adv Protein Chem. 1966;21:113-286
5333288
J Immunol. 1976 Aug;117(2):661-5
820804
Annu Rev Biochem. 1975;44:697-724
1094920
Biochemistry. 1976 AUG 24;15(17):3884-90
952891
Immunochemistry. 1976 Apr;13(4):329-32
939577
J Immunol. 1976 Feb;116(2):319-26
1249417
J Biol Chem. 1975 Jul 25;250(14):5459-69
1141239
J Biol Chem. 1967 Jan 25;242(2):306-11
6016615
J Biol Chem. 1965 Dec;240(12):4740-5
5846989