Complete amino acid sequence of rabbit muscle glycogen phosphorylase.
The sequence of the 841 amino acid residues in each subunit (molecular weight 97,412) of rabbit muscle glycogen phosphorylase b (1,4-alpha-D-glucan:orthophosphate alpha-glucosyltransferase; EC 2.4.1.1) has been determined. The general strategy was based on limited proteolysis of native phosphorylase b by subtilisin BPN', yielding two large segments (light and heavy) which were fragmented by cleavage at methyonyl-, asparaginyl-glycine, and aspartyl-proline bonds. Analysis of two cyanogen bromide fragments (CB14 and CB17) isolated from the intact molecule yielded the overlap between the light and heavy fragments and the remainder of the sequence. The residues involved in the covalent and allosteric control of the enzyme, and in the binding of the cofactor pyridoxal 5'-phosphate, were identified as serine-14, tyrosine-155, and lysine-679, respectively.
D000595:Amino Acid Sequence; D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D003488:Cyanogen Bromide; D006868:Hydrolysis; D009132:Muscles / Q000201:enzymology*; D010446:Peptide Fragments / Q000032:analysis; D006005:Phosphorylases; D011817:Rabbits
D010446:Peptide Fragments; D006005:Phosphorylases; D003488:Cyanogen Bromide
J Biochem. 1964 Sep;56:222-9
14228496
Biochemistry. 1964 Apr;3:542-51
14188171
J Biol Chem. 1963 Feb;238:622-7
14023808
J Biol Chem. 1958 Mar;231(1):65-71
13538948
J Biol Chem. 1976 Oct 10;251(19):6142-6
184094
Biochemistry. 1971 Aug 3;10(16):3132-40
5126929
Biochemistry. 1970 Sep 1;9(18):3591-8
5534315
J Mol Biol. 1974 Dec 25;90(4):703-17
4449138
Biochemistry. 1973 Dec 4;12(25):5154-61
4799824
Biochemistry. 1973 Dec 18;12(26):5225-32
4796797
Biochemistry. 1972 Dec 19;11(26):4974-7
4404720
Biochemistry. 1973 May 8;12(10):1901-6
4350104
Biochemistry. 1969 May;8(5):2161-71
4306642
FEBS Lett. 1975 Jul 15;55(1):120-3
1140412
Proc Natl Acad Sci U S A. 1972 Dec;69(12):3506-9
4509307
Biochemistry. 1972 Nov 21;11(24):4493-502
4675874
J Biol Chem. 1970 Apr 25;245(8):1895-902
4985905
J Biol Chem. 1968 Dec 10;243(23):6202-9
4881359
Biochemistry. 1971 Jul 6;10(14):2683-94
5558692
Essays Biochem. 1970;6:23-68
5499234
J Biol Chem. 1970 Apr 10;245(7):1558-65
5438354
Biochemistry. 1970 Jun 9;9(12):2408-21
5423260
J Biol Chem. 1969 Oct 25;244(20):5597-607
5348603
Biochemistry. 1967 Oct;6(10):3315-27
6069861
Eur J Biochem. 1967 Mar;1(1):80-91
6059350