Transfer of codominant markers by isolated metaphase chromosomes in Chinese hamster ovary cells.
Codominant mutations to methotrexate and ouabain resistance in Chinese hamster ovary cells can be transferred to recipient Chinese hamster ovary cells by isolated metaphase chromosomes. For methotrexate, both the structural change and the increased activity of dihydrofolate reductase (5,6,7,8-tetrahydrofolate:NADP+ oxidoreductase EC 1.5.1.3), characteristic of the donor cells, are observed in the transferents (cells that carry and express functions derived by chromosome transfer). The transferents are unstable in the absence of selection although stable clones can be isolated. From results obtained by fractionation of chromosomes and transfer to recipients, the methotrexate and ouabain markers can be assigned to the middle and large size-classes of chromosomes, respectively. By fractionation and transfer of chromosomes, from transferents to new recipients, evidence has been obtained that chromosome integration is not restricted to a particular chromosomal site in the recipient.
D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D002875:Chromosomes / Q000187:drug effects / Q000502:physiology*; D004351:Drug Resistance; D005799:Genes, Dominant; D008677:Metaphase / Q000187:drug effects; D008727:Methotrexate / Q000494:pharmacology; D009154:Mutation; D010042:Ouabain / Q000494:pharmacology; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics
D010042:Ouabain; D013762:Tetrahydrofolate Dehydrogenase; D008727:Methotrexate
Can J Biochem. 1977 Apr;55(4):445-52
15711
Exp Cell Res. 1976 Nov;103(1):15-22
186287
Cell. 1976 Oct;9(2):269-87
184963
In Vitro. 1976 Nov;12(11):749-57
1035580
Somatic Cell Genet. 1976 May;2(3):245-61
1028172
Cell. 1976 Oct;9(2):213-9
975243
Somatic Cell Genet. 1975 Apr;1(2):137-51
1235904
Proc Natl Acad Sci U S A. 1976 Apr;73(4):1274-8
177984
Proc Natl Acad Sci U S A. 1972 Nov;69(11):3119-23
4508306
Proc Natl Acad Sci U S A. 1973 Apr;70(4):1258-62
4515624
Nat New Biol. 1971 Mar 10;230(10):52-4
5279808
Virology. 1974 Sep;61(1):303-5
4369749
Cell. 1975 Feb;4(2):167-72
1168545
J Mol Biol. 1968 Feb 28;32(1):101-12
4230571
Proc Natl Acad Sci U S A. 1975 May;72(5):1792-6
1057170
Cell. 1974 Jul;2(3):173-82
4547236
Proc Natl Acad Sci U S A. 1976 Aug;73(8):2838-42
1066695
Proc Natl Acad Sci U S A. 1975 May;72(5):1797-801
1057770
Cell. 1975 Oct;6(2):121-8
1182798
Exp Cell Res. 1970 Mar;59(3):469-78
5461579