Immunogenicity of liposomal model membranes in mice: dependence on phospholipid composition.
This investigation demonstrated that antigenic expression in liposomal model membranes may be markedly influenced by phospholipid composition. Incorporation of dinitrophenylaminocaproylphosphatidylethanolamine (Dnp-Cap-PE) into egg lecithin/cholesterol/dicetylphosphate bilayers did not significantly enhance the response of AKR mice to this synthetic amphipathic antigen. In contrast, the immunogenicity of Dnp-Cap-PE was increased (as measured by either plaque-forming cell frequency of hemagglutination titer) after its insertion into liposomes prepared with beef sphingomyelin instead of egg lecithin. We also show that the response to the Dnp-Cap determinant can be stimulated by the presence of lipid A in the same bilayers without altering the relative immunogenic potency of the sphingomyelin and lecithin liposomes; similarly, incorporation of this mitogen into sphingomyelin liposomes produced a greater polyclonal (nonspecific) response. The response to Dnp-Cap-PE-sensitized liposomes (with or without lipid A) prepared with a series of synthetic phosphatidylcholines (distearoyl-, dipalmitoyl-, dimyristoyl-, dilauroyl-, dioleoyl-) suggests a direct correlation between liposomal immunogenicity and transition temperature of the phospholipid.
D000818:Animals; D000917:Antibody Formation; D004136:Dinitrobenzenes / Q000276:immunology; D008081:Liposomes / Q000276:immunology*; D008297:Male; D051379:Mice; D008806:Mice, Inbred AKR; D010713:Phosphatidylcholines / Q000276:immunology / Q000502:physiology; D010743:Phospholipids / Q000276:immunology*; D013109:Sphingomyelins / Q000276:immunology; D013329:Structure-Activity Relationship; D013696:Temperature
D004136:Dinitrobenzenes; D008081:Liposomes; D010713:Phosphatidylcholines; D010743:Phospholipids; D013109:Sphingomyelins
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