Surface antigen in early differentiation.
Addition of Fab fragments from rabbit antiserum to surface antigen F9 to 2-cell stage mouse embryos in culture does not alter cleavage; however, the addition prevents culture does not alter cleavage; however, the addition prevents the formation of compact morulae and blastocysts. A similar effect is observed when Fab fragments are added to already compact 8-cell stage or even older morulae, but disappears at the beginning of blastocoel formation. This effect is reversible: uncompact 30-cell embryos washed free of Fab become compact in a few hours, produce blastocysts, and upon reimplantation into pseudopregnant mothers can produce mice. Development is not altered by divalent anti-F9 antibodies, by Fab fragments from sera directed against other embryo surface antigens, or by succinyl concanavalin A.
D000818:Animals; D000941:Antigens / Q000032:analysis*; D001755:Blastocyst / Q000276:immunology; D002454:Cell Differentiation; D002460:Cell Line; D004622:Embryo, Mammalian / Q000276:immunology* / Q000648:ultrastructure; D005260:Female; D007140:Immunoglobulin Fab Fragments / Q000302:isolation & purification; D007074:Immunoglobulin G / Q000302:isolation & purification; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008815:Mice, Inbred Strains / Q000276:immunology; D011247:Pregnancy; D011817:Rabbits / Q000276:immunology
D000941:Antigens; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G
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