Molecular weight of bacteriorhodopsin solubilized in Triton X-100.
Bacteriorhodopsin from Halobacterium halobium has been solubilized in the nonionic detergent Triton X-100. The circular dichroic spectrum and hydrodynamic properties indicate that the structure of this protein in the detergent is not significantly altered from that of the native membrane-bound form. Bacteriorhodopsin is monomeric under the conditions of solubilization with a molecular weight of 24,250+/-2,000 and binds approximately one micelle of Triton X-100.
D001436:Bacteriorhodopsins; D002338:Carotenoids; D002942:Circular Dichroism; D008970:Molecular Weight; D011092:Polyethylene Glycols; D012995:Solubility; D013057:Spectrum Analysis; D014461:Ultracentrifugation
D002338:Carotenoids; D011092:Polyethylene Glycols; D001436:Bacteriorhodopsins
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