Differences in removal of acetylaminofluorene and pyrimidine dimers from the DNA of cultured mammalian cells.
The rate and extent of disappearance of two DNA lesions (pyrimidine dimers and covalently bound acetylaminofluorene), both thought to be removed by the so-called wide-patch (approximately 100 nucleotides) repair process, were studied in a variety of cultured mammalian cells. With the exception of mouse cells, dimers were removed more rapidly and extensively than covalently bound acetylaminofluorene. In human cells, for example, about 50% of the dimers were excised from DNA in 1 hr while only 25-50% of the chemically induced lesions were excised from DNA after 48 hr. Surprisingly mouse cells, which remove few dimers, were about as competent as control human fibroblasts at removing acetylaminofluorene lesions; however, xeroderma pigmentosum cells (group D) removed fewer N-acetoxy-2-acetylaminofluorene-induced lesions than control human cells. Our data raise the possibility of separate repair processes for these two types of lesions and suggest that their expression may be under similar genetic control in human cells.
D015073:2-Acetylaminofluorene / Q000494:pharmacology*; D000099:Acetoxyacetylaminofluorene / Q000494:pharmacology; D002449:Cell Aggregation / Q000187:drug effects; D002460:Cell Line; D004260:DNA Repair; D004261:DNA Replication / Q000187:drug effects* / Q000528:radiation effects; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D005449:Fluorenes / Q000494:pharmacology*; D007700:Kinetics; D011740:Pyrimidine Dimers; D014466:Ultraviolet Rays
D005449:Fluorenes; D011740:Pyrimidine Dimers; D000099:Acetoxyacetylaminofluorene; D015073:2-Acetylaminofluorene
Chem Biol Interact. 1976 Oct 2;15(2):149-64
975399
Biochem Biophys Res Commun. 1977 Jan 10;74(1):285-90
836285
Biochemistry. 1967 Jan;6(1):177-82
6030315
Mutat Res. 1974 Dec;25(3):383-90
4373650
Cancer Res. 1974 Dec;34(12):3318-25
4371955
Cancer Res. 1976 Apr;36(4):1397-403
1260764
Biochim Biophys Acta. 1973 Oct 12;324(2):206-17
4271371
Int Rev Cytol. 1976;45:1-23
783064
Exp Cell Res. 1972 Sep;74(1):67-80
4627418
Anal Biochem. 1973 May;53(1):124-31
4575942
Cancer Res. 1973 Sep;33(9):2097-103
4542003
Cancer Res. 1973 Feb;33(2):362-9
4540129
Biophys J. 1974 Oct;14(10):791-803
4473053
Exp Cell Res. 1974 Sep;88(1):47-55
4472970
Biochemistry. 1974 Jul 16;13(15):3018-23
4842274
Mutat Res. 1973 Aug;19(2):231-5
4748978
Mutat Res. 1973 Jan;17(1):127-37
4682598
Eur J Biochem. 1972 Dec 18;31(3):438-45
4675366
Cancer Res. 1972 Sep;32(9):1855-60
4641277
Cancer Res. 1972 Oct;32(10):2042-8
5080759
Biochem Biophys Res Commun. 1972 Jan 31;46(2):1019-24
5057877
Basic Life Sci. 1975;5B:763-71
811213
Proc Natl Acad Sci U S A. 1976 May;73(5):1466-70
1064020
Proc Natl Acad Sci U S A. 1976 Apr;73(4):1169-73
1063398
Proc Natl Acad Sci U S A. 1975 Jan;72(1):103-7
1054487
J Natl Cancer Inst. 1975 Jun;54(6):1287-94
1133846
Proc Natl Acad Sci U S A. 1969 Nov;64(3):1035-41
5264135
Chem Biol Interact. 1971 Feb;3(1):49-68
5156326
Cancer Res. 1971 Sep;31(9):1297-306
5115057
Mutat Res. 1971 Aug;12(4):453-62
5097249