title: Synthesis and secretion of a high molecular weight form of nerve growth factor by skeletal muscle cells in culture.
abstract: Rat skeletal muscle cells and a cloned myogenic cell line synthesize and secrete in culture a molecule that is immunologically and biologically indistinguishable from the active form of nerve growth factor (NGF) from mouse submandibular gland. This protein can be detected in medium conditioned by muscle cells both before and after fusion and in the soluble fraction of muscle cell homogenates. Chromatographic data also reveal that the molecular properties of muscle cell NGF differ from those of the growth factor purified from mouse submandibular glands. Muscle cell NGF has a molecular weight between 140,000 and 160,000, whereas purified mouse gland NGF has a molecular weight of 26,000. The biologic function of muscle cell NGF is not known, although it could be that it plays some role relating to the association of nerves and muscle in vivo.
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002850:Chromatography, Gel; D007739:L Cells; D051379:Mice; D008970:Molecular Weight; D009132:Muscles / Q000378:metabolism*; D009414:Nerve Growth Factors / Q000096:biosynthesis* / Q000378:metabolism; D051381:Rats
chemical_list: D009414:Nerve Growth Factors
references.citation: J Natl Cancer Inst. 1956 Jun;16(6):1375-403
references.pmid: 13320119
references.citation: Biochemistry. 1977 Apr 5;16(7):1525-30
references.pmid: 849428
references.citation: Proc Natl Acad Sci U S A. 1977 Apr;74(4):1492-6
references.pmid: 266190
references.citation: Proc Natl Acad Sci U S A. 1974 Apr;71(4):1554-8
references.pmid: 4524658
references.citation: Cancer Res. 1970 Nov;30(11):2627-35
references.pmid: 5530557
references.citation: Dev Biol. 1971 May;25(1):1-29
references.pmid: 4397658
references.citation: Dev Biol. 1972 Jun;28(2):407-29
references.pmid: 4338026
references.citation: Science. 1973 Nov 9;182(4112):588-9
references.pmid: 4270498
references.citation: Biochemistry. 1973 Jan 2;12(1):100-15
references.pmid: 4566923
references.citation: J Biol Chem. 1976 Jan 25;251(2):459-64
references.pmid: 1394
references.citation: Dev Biol. 1973 Mar;31(1):147-62
references.pmid: 4787184
references.citation: Proc Natl Acad Sci U S A. 1969 Oct;64(2):787-94
references.pmid: 4982360
references.citation: Proc Natl Acad Sci U S A. 1975 May;72(5):1895-8
references.pmid: 1057179
references.citation: Science. 1976 Jul 30;193(4251):371-7
references.pmid: 935873
references.citation: Science. 1975 Jan 31;187(4174):361-2
references.pmid: 1167427
references.citation: Proc Natl Acad Sci U S A. 1968 Oct;61(2):477-83
references.pmid: 5245982
references.citation: Exp Cell Res. 1971 May;66(1):33-48
references.pmid: 5104198
references.citation: Biochemistry. 1968 Apr;7(4):1296-303
references.pmid: 5677819