title: Studies on nucleic acid reassociation kinetics: rate of hybridization of excess RNA with DNA, compared to the rate of DNA renaturation.
abstract: The rate of reaction of double-stranded replicative form (RF) [3H]DNA of bacteriophage phiX174 with excess (+)strand DNA and (+)strand RNA was measured by standard methods of hydroxyapatite chromatography. The reactions follow pseudo-first-order kinetics and the observed rate constant for the RNA-DNA reaction differs less than 25% from that of the DNA-DNA reaction. The pseudo-first-order rate constants are close to the value predicted on the basis of the second-order rate constant measured in the renaturation of the double-stranded phiX RF [3H]DNA.
mesh_terms: D003090:Coliphages; D004279:DNA, Viral; D007700:Kinetics; D009693:Nucleic Acid Hybridization; D009695:Nucleic Acid Renaturation; D009696:Nucleic Acids
chemical_list: D004279:DNA, Viral; D009696:Nucleic Acids
references.citation: Nature. 1976 Nov 4;264(5581):34-41
references.pmid: 1004533
references.citation: J Mol Biol. 1974 Feb 15;83(1):47-61
references.pmid: 4594147
references.citation: J Cell Biol. 1971 Sep;50(3):774-86
references.pmid: 4999767
references.citation: J Mol Biol. 1973 Jul 15;77(4):495-500
references.pmid: 4579448
references.citation: Nat New Biol. 1971 May 5;231(18):8-12
references.pmid: 4932893
references.citation: J Mol Biol. 1976 Jun 5;103(4):681-97
references.pmid: 781282
references.citation: J Virol. 1974 Oct;14(4):872-7
references.pmid: 4609216
references.citation: Cell. 1974 May;2(1):9-20
references.pmid: 4411655
references.citation: Science. 1968 Aug 9;161(3841):529-40
references.pmid: 4874239
references.citation: Proc Natl Acad Sci U S A. 1976 Feb;73(2):415-9
references.pmid: 1108024
references.citation: Proc Natl Acad Sci U S A. 1975 Dec;72(12):4805-9
references.pmid: 1108002
references.citation: Cell. 1975 Mar;4(3):217-38
references.pmid: 1122554
references.citation: J Mol Biol. 1970 Oct 28;53(2):251-9
references.pmid: 5493279
references.citation: Nucleic Acids Res. 1974 Nov;1(11):1521-9
references.pmid: 10793707