Molecular properties of the nerve growth factor secreted by L cells.
The molecular size and stability of the nerve growth factor (NGF) secreted in culture by L cells have been studied by sedimentation and gel filtration chromatography. Results indicate that L cell NGF has a molecular weight of 160,000. It contains as part of its structure a protein component that is biologically, immunologically, and electrophoretically indistinguishable from the biologically active factor purified from mouse submandibular glands. However, unlike pure gland NGF, L cell NGF is highly stable in solution, and this finding indicates that L cell NGF is a form of the factor different from that previously described.
D000818:Animals; D001681:Biological Assay; D002642:Chick Embryo; D004591:Electrophoresis, Polyacrylamide Gel; D007739:L Cells / Q000378:metabolism*; D009414:Nerve Growth Factors / Q000302:isolation & purification / Q000378:metabolism
D009414:Nerve Growth Factors
J Cell Biol. 1977 Mar;72(3):769-73
838775
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1554-8
4524658
Annu Rev Biochem. 1976;45:531-58
786157
Biochemistry. 1973 Jan 2;12(1):100-15
4566923
J Biol Chem. 1976 Jan 25;251(2):459-64
1394
Proc Natl Acad Sci U S A. 1969 Oct;64(2):787-94
4982360
Physiol Rev. 1968 Jul;48(3):534-69
4875350
J Exp Zool. 1965 Nov;160(2):183-8
5327556
Biochem Pharmacol. 1976 Jul 1;25(13):1445-9
782456
Proc Natl Acad Sci U S A. 1975 May;72(5):1895-8
1057179
J Neurochem. 1975 Dec;25(6):843-51
1206401
Science. 1975 Jan 31;187(4174):361-2
1167427
Proc Natl Acad Sci U S A. 1967 Jun;57(6):1782-9
5231412
Biochemistry. 1971 Feb 2;10(3):463-9
5543976
Arch Biochem Biophys. 1969 Mar;130(1):337-46
5778650
Biochemistry. 1968 Apr;7(4):1296-303
5677819
Biochemistry. 1967 Jul;6(7):2202-9
6049455