Adenylate cyclase from synchronized neuroblastoma cells: responsiveness to prostaglandin E1, adenosine, and dopamine during the cell cycle.
Neuroblastoma cells were synchronized by a combined isoleucine plus glutamine starvation. Adenylate cyclase activity [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] was measured under basal conditions and in the presence of dopamine, adenosine and prostaglandin (PG) E1. A clear dissociation occurred between the respective evolution patterns of basal and agonist-stimulated adenylate cyclase activities. The magnitudes of the enzyme response to PGE1, adenosine, and dopamine also exhibited different evolution patterns during the cell cycle. Evolution of adenylate cyclase responsiveness to PGE1 during the cell cycle exhibited striking similarities with the intracellular 3':5'-cyclic AMP changes observed elsewhere. Use of theophylline and fluphenazine as specific inhibitors of adenosine and dopamine, respectively, made it possible to demonstrate that adenosine, dopamine, and PGE1 stimulated adenylate cyclase through independent receptor sites. Furthermore, whatever the stage of the cell cycle, responses to these three agonists were not additive, indicating that the receptors of adenosine, dopamine, and PGE1 control the same adenylate cyclase moieties. The data suggest that adenylate cyclase cell content and enzyme responsiveness to specific agonists can be independently controlled.
D000241:Adenosine / Q000494:pharmacology*; D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D004261:DNA Replication; D004298:Dopamine / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D005476:Fluphenazine / Q000494:pharmacology; D007700:Kinetics; D051379:Mice; D009447:Neuroblastoma / Q000201:enzymology* / Q000378:metabolism; D011458:Prostaglandins E / Q000494:pharmacology*; D013806:Theophylline / Q000494:pharmacology
D011458:Prostaglandins E; D013806:Theophylline; D000262:Adenylyl Cyclases; D000241:Adenosine; D005476:Fluphenazine; D004298:Dopamine
Exp Cell Res. 1974 Feb;83(2):335-43
4361423
Nat New Biol. 1971 Sep 29;233(39):141-2
4107316
J Biol Chem. 1974 Dec 25;249(24):7848-56
4372220
FEBS Lett. 1973 Oct 15;36(2):193-8
4356788
Exp Cell Res. 1974 Mar 15;84(1):40-6
4361721
Proc Natl Acad Sci U S A. 1972 Feb;69(2):456-8
4333986
Nat New Biol. 1972 Mar 15;236(63):49-52
4502426
Nat New Biol. 1972 Oct 11;239(93):161-3
4349672
Proc Natl Acad Sci U S A. 1972 Jan;69(1):258-63
4400294
Proc Natl Acad Sci U S A. 1974 May;71(5):1590-3
4365570
Proc Natl Acad Sci U S A. 1974 Dec;71(12):4920-4
4373735
Biochem Biophys Res Commun. 1972 Dec 18;49(6):1645-9
4344815
Proc Natl Acad Sci U S A. 1973 May;70(5):1432-6
4351178
Proc Natl Acad Sci U S A. 1973 Jun;70(6):1659-63
4352647
Science. 1975 Jun 13;188(4193):1084-9
2976
J Neurochem. 1976 Feb;26(2):265-73
176321
Annu Rev Biochem. 1975;44:491-522
166606