title: Amino acid sequence of honeybee prepromelittin synthesized in vitro.
abstract: Translation of melittin messenger RNA from queen bee venom glands in a cell-free system from wheat germ yielded prepromelittin. Sequence analysis of the labeled in vitro product was performed by automatic Edman degradation of the intact polypeptide as well as by analysis of some of its proteolytic fragments. Prepromelittin was shown to be composed of 70 amino acids, two of which have not been identified. The sequence of melittin is located in the COOH-terminal third of the polypeptide chain (residues 44--69). Prepromelittin starts with a very hydrophobic pre-region, probably 21 residues long, followed by a pro-part of unusual sequence, containing only alanine, proline, and acidic residues. At least three post-translational reactions are required to convert prepromelittin to mellitin.
mesh_terms: D000595:Amino Acid Sequence; D001514:Bee Venoms / Q000378:metabolism*; D001516:Bees; D002474:Cell-Free System; D066298:In Vitro Techniques; D008555:Melitten / Q000378:metabolism*; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000096:biosynthesis
chemical_list: D001514:Bee Venoms; D011498:Protein Precursors; D008555:Melitten; C016694:prepromelittin
references.citation: Proc Natl Acad Sci U S A. 1977 Feb;74(2):716-20
references.pmid: 403522
references.citation: Proc Natl Acad Sci U S A. 1977 May;74(5):2059-63
references.pmid: 325565
references.citation: Proc Natl Acad Sci U S A. 1977 Apr;74(4):1358-62
references.pmid: 266178
references.citation: Proc Natl Acad Sci U S A. 1977 Mar;74(3):975-8
references.pmid: 265590
references.citation: J Biol Chem. 1977 Sep 25;252(18):6276-9
references.pmid: 893407
references.citation: Biochem J. 1977 Jan 1;161(1):189-92
references.pmid: 851420
references.citation: Biochem Biophys Res Commun. 1977 Jan 10;74(1):106-12
references.pmid: 836272
references.citation: J Mol Biol. 1976 Jul 25;105(1):177-81
references.pmid: 994185
references.citation: J Mol Biol. 1974 Aug 15;87(3):451-62
references.pmid: 4444031
references.citation: Nat New Biol. 1972 Sep 27;239(91):117-20
references.pmid: 4507519
references.citation: Eur J Biochem. 1973 Mar 15;33(3):558-66
references.pmid: 4571501
references.citation: Proc Natl Acad Sci U S A. 1974 Sep;71(9):3731-5
references.pmid: 4530333
references.citation: J Cell Biol. 1975 Dec;67(3):835-51
references.pmid: 811671
references.citation: Proc Natl Acad Sci U S A. 1976 Jun;73(6):1964-8
references.pmid: 778852
references.citation: Proc Natl Acad Sci U S A. 1975 Dec;72(12):5016-20
references.pmid: 1061088
references.citation: Proc Natl Acad Sci U S A. 1976 Jan;73(1):29-33
references.pmid: 1061124
references.citation: Biochemistry. 1976 Jan 13;15(1):20-5
references.pmid: 1247507
references.citation: Biochem Biophys Res Commun. 1975 Dec 1;67(3):1114-21
references.pmid: 1201063
references.citation: Eur J Biochem. 1975 Dec 1;60(1):309-15
references.pmid: 1204644
references.citation: Biochem Biophys Res Commun. 1975 Jan 6;62(1):103-9
references.pmid: 1111509
references.citation: Hoppe Seylers Z Physiol Chem. 1967 Jan;348(1):37-50
references.pmid: 5592400
references.citation: Nucleic Acids Res. 1974 Nov;1(11):1385-97
references.pmid: 10793698