Nonexistence of N-acetylseryl-tRNA in rat liver.
Reports of the existence of N-acetylseryl-tRNA in rat liver were reinvestigated. Methods were developed to permit recovery of N-acetylserine in a yield of 30--40% from N-acetylseryl-tRNA added to liver homogenates and cell-free incubations. [14C]Serine and [3H]acetate were injected into rats pretreated with iron and into rats after partial hepatectomy, and aminoacyl-tRNA was isolated from their livers. The amount of radioactivity associated with N-acetylserine in the amino acids released by hydrolysis from the aminoacyl-tRNA was negligible. No formation of N-acetylseryl-tRNA could be observed in incubations of acetyl CoA and seryl-tRNA or tRNA with enzyme fractions from liver of rats pretreated with iron. It is concluded that previous reports of the existence of N-acetylseryl-tRNA in rat liver are erroneous.
D000818:Animals; D003600:Cytosol / Q000032:analysis; D008099:Liver / Q000032:analysis*; D008297:Male; D008862:Microsomes, Liver / Q000032:analysis; D012346:RNA, Transfer, Amino Acyl / Q000032:analysis*; D051381:Rats; D012694:Serine / Q000031:analogs & derivatives* / Q000032:analysis
D012346:RNA, Transfer, Amino Acyl; C018441:tRNA, N-acetylserine-; D012694:Serine