title: Microviscosity modulation during the cell cycle of neuroblastoma cells.
abstract: Microviscosity (n) of the cell membrane lipid layer was determined in synchronized C1300 mouse neuroblastoma cells (clone Neuro-2A) by fluorescence polarization of 1,6-diphenystratum. The determined n value was maximal in mitosis, decreased markedly in the G1 phase, remained constant at a low level during the S phase, and increased again during the G2 phase. These findings imply a direct role of the cell membrane fluidity in regulation of the cell cycle.
mesh_terms: D000818:Animals; D002453:Cell Cycle; D002462:Cell Membrane / Q000032:analysis; D002999:Clone Cells; D008563:Membrane Lipids; D051379:Mice; D008856:Microscopy, Fluorescence; D009447:Neuroblastoma / Q000032:analysis / Q000473:pathology; D013050:Spectrometry, Fluorescence; D013696:Temperature; D013997:Time Factors; D014783:Viscosity
chemical_list: D008563:Membrane Lipids
references.citation: Proc Natl Acad Sci U S A. 1977 Mar;74(3):1072-6
references.pmid: 265552
references.citation: Proc Natl Acad Sci U S A. 1976 Dec;73(12):4526-30
references.pmid: 1070002
references.citation: Proc Natl Acad Sci U S A. 1977 Mar;74(3):1110-4
references.pmid: 265557
references.citation: Eur J Immunol. 1975 Mar;5(3):166-70
references.pmid: 1234052
references.citation: Biochim Biophys Acta. 1972 Sep 1;282(1):277-92
references.pmid: 4341789
references.citation: J Biol Chem. 1974 Apr 25;249(8):2652-7
references.pmid: 4822508
references.citation: J Mol Biol. 1974 Jan 5;85(4):603-15
references.pmid: 4851371
references.citation: Annu Rev Biophys Bioeng. 1974;3(0):179-201
references.pmid: 4371655
references.citation: Proc Natl Acad Sci U S A. 1973 Dec;70(12):3802-5
references.pmid: 4359490
references.citation: Nature. 1971 Oct 13;233(5320):219-20
references.pmid: 4938853
references.citation: Nat New Biol. 1972 Oct 11;239(93):161-3
references.pmid: 4349672
references.citation: Biochim Biophys Acta. 1975 Nov 17;413(1):143-56
references.pmid: 90
references.citation: Biochim Biophys Acta. 1976 Apr 16;433(1):133-49
references.pmid: 1260056
references.citation: Exp Cell Res. 1974 Oct;88(2):311-8
references.pmid: 4372073
references.citation: Nature. 1973 Nov 30;246(5431):291-5
references.pmid: 4271386
references.citation: Proc Natl Acad Sci U S A. 1975 Sep;72(9):3433-7
references.pmid: 1059128
references.citation: Ann N Y Acad Sci. 1974;238:420-35
references.pmid: 4613241
references.citation: Biochim Biophys Acta. 1976 Apr 30;458(1):1-72
references.pmid: 181093
references.citation: J Cell Biol. 1973 Jun;57(3):815-36
references.pmid: 4735453
references.citation: Proc Natl Acad Sci U S A. 1975 Sep;72(9):3351-4
references.pmid: 1059120
references.citation: Biochim Biophys Acta. 1976 Apr 13;457(1):57-108
references.pmid: 1260065
references.citation: Exp Cell Res. 1975 Aug;94(1):159-75
references.pmid: 1193125