Determination of catecholamines and their 3-O-methylated metabolites in urine by mass fragmentography with use of deuterated internal standards.
We report the determination of catecholamines (dopamine, norepinephrine, and epinephrine) and their 3-O-methylated metabolites (3-methoxytyramine, normetanephrine, and metanephrine) in urine by gas chromatography/mass spectrometry, with use of stable isotopically labeled internal standards. Normal excretion values in terms of creatinine, expressed as a function of age, are given, together with results obtained for patients with neuroblastoma, ganglioneuroblastoma, pheochromocytoma, parkinsonism treated with L-DOPA plus peripheral decarboxylase inhibitor, and a patient with cardiogenic shock treated with dopamine. The results obtained for normal adults are discussed in relation to other catecholamine metabolites and mean overall catecholamine turnover.
D000293:Adolescent; D000310:Adrenal Gland Neoplasms / Q000652:urine; D000328:Adult; D000367:Age Factors; D000368:Aged; D002395:Catecholamines / Q000652:urine*; D002648:Child; D002675:Child, Preschool; D003903:Deuterium; D004298:Dopamine / Q000652:urine; D004837:Epinephrine / Q000652:urine; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008745:Methylation; D008875:Middle Aged; D009447:Neuroblastoma / Q000652:urine; D009638:Norepinephrine / Q000652:urine; D010300:Parkinson Disease / Q000652:urine; D010673:Pheochromocytoma / Q000652:urine; D012016:Reference Values
D002395:Catecholamines; D003903:Deuterium; D004298:Dopamine; D009638:Norepinephrine; D004837:Epinephrine