Structure of histone H1-DNA complex: effect of histone H1 on DNA condensation.
Large doughnut-shaped complexes were formed when histone H1 was mixed with DNA in amounts that extensively neutralized it. The doughnut shape is the most prevalent from observed by electron microscopy for linear double-stranded DNA independent of the molecular weight of the DNA in the range 1.2 x 10(6) to 25 x 10(6) at an H1/DNA input weight ratio of 1.3, at ionic strength 0.17. Doughnuts were not observed for single-stranded DNA-H1 complexes; instead, the complexes were globular. The circumference of the doughnutshaped particles indicates that much of the rigidity of duplex DNA in the complex has remained. Evidently, the condensation of the nucleohistone is constrained by the rigidity of duplex DNA and, under this constraint, surface contact with water is minimized by adopting a doughnut shape. Histone H5 causes a type of DNA condensation similar to that of H1 at comparable charge ratios. Core histones H2A-H2b, H3, and H4 complex with DNA to form globular aggregates of such small diameter that the duplex DNA in them must be much more tightly folded than is the case with the doughnut-shaped complexes. Because these histones are designed to fold DNA into nucleosomes 100 A wide, they must destroy the rigidity of free duplex DNA, perhaps by forming kinks in the chain.
D000818:Animals; D055598:Chemical Phenomena; D002621:Chemistry; D004247:DNA; D006657:Histones; D008722:Methods; D008854:Microscopy, Electron; D008968:Molecular Conformation; D009391:Nephelometry and Turbidimetry; D011817:Rabbits
D006657:Histones; D004247:DNA
J Bacteriol. 1976 Apr;126(1):447-53
770430
Nucleic Acids Res. 1976 Sep;3(9):2353-66
787935
Proc Natl Acad Sci U S A. 1976 Dec;73(12):4382-6
826906
J Mol Biol. 1977 Jan 25;109(3):393-404
833849
Biochem Biophys Res Commun. 1976 Jul 12;71(1):196-202
962913
Cell. 1976 Dec;9(4 Pt 1):627-32
1009580
Proc Natl Acad Sci U S A. 1975 Nov;72(11):4288-92
1060108
Proc Natl Acad Sci U S A. 1976 Sep;73(9):3068-72
1067602
Annu Rev Biochem. 1975;44:725-74
1094921
Nature. 1975 Jun 12;255(5509):530-3
1095931
FEBS Lett. 1975 Feb 1;50(2):195-9
1112411
FEBS Lett. 1975 Feb 1;50(2):200-3
1112412
Nature. 1975 Jan 24;253(5489):245-9
1167623
Nucleic Acids Res. 1975 Oct;2(10):1751-70
1187345
Nature. 1976 Jan 29;259(5541):333-5
1250371
Nucleic Acids Res. 1976 Jan;3(1):117-27
1250693
Nucleic Acids Res. 1976 Feb;3(2):477-92
1257057
J Mol Biol. 1969 Aug 14;43(3):439-60
4100471
Science. 1974 Jan 25;183(4122):330-2
4128918
J Mol Biol. 1967 Apr 28;25(2):299-315
4291873
Eur J Biochem. 1969 Dec;11(3):495-8
4312785
Proc Natl Acad Sci U S A. 1972 Nov;69(11):3370-4
4343968
Science. 1974 May 24;184(4139):868-71
4825889
Biochemistry. 1972 Apr 11;11(8):1422-30
5063176
J Mol Biol. 1971 May 14;57(3):437-55
5103972
Eur J Biochem. 1971 Sep 24;22(2):235-45
5116611
Proc Natl Acad Sci U S A. 1970 Nov;67(3):1616-23
5274484
Proc Natl Acad Sci U S A. 1971 Aug;68(8):1886-90
5288774
Biochim Biophys Acta. 1970 May 21;209(1):54-7
5421975
Biochemistry. 1970 Jul 7;9(14):2814-22
5459534
J Biol Chem. 1970 Sep 10;245(17):4286-92
5498413
Biochemistry. 1970 Oct 27;9(22):4410-6
5528747
J Mol Biol. 1966 Feb;15(2):409-19
5915175
J Gen Physiol. 1957 Jan 20;40(3):451-90
13398575
Biochim Biophys Acta. 1962 Aug 27;62:608-9
14480271
Science. 1975 Mar 28;187(4182):1202-3
17754289