Inhibition of cytokinesis in Friend leukemia cells by membrane mobility agents.
Treatment of a line of Friend leukemia cells with a dispersion of the membrane mobility agent, A2C, yields cells that undergo successive nuclear divisions without cytokinesis, resulting eventually in cells with as many as 30 nuclei. Neither the DNA replication rate of the cells nor the generation time is different after treatment; in addition, the multiple nuclei divide synchronously, and the chromosome number corresponds to the number of nuclei in the cell. Inhibition of cytokinesis is not observed if the cells are washed with reagent-free medium within 1 hr of treatment, but is observed if washing is delayed for 24 hr. Membrane mobility agent loaded with the fluorescent probe, Flomol F20C, leads to fluorescent membrane; fluorescence disappears from the membrane after a change of medium within 1 hr, but not after a change of medium within 24 hr. Some stages in the overall development resemble those seen for cytochalasin B inhibition of cytokinesis, although the mechanisms may well be different for the inhibition promoted by membrane mobility agent. The inhibition of cytokinesis by A2C provides a potentially interesting means of studying cytokinesis and the regulation of differentiation.
D000818:Animals; D002455:Cell Division / Q000187:drug effects*; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects; D002467:Cell Nucleus / Q000648:ultrastructure; D004273:DNA, Neoplasm / Q000096:biosynthesis; D005227:Fatty Acids / Q000494:pharmacology*; D005456:Fluorescent Dyes; D004915:Leukemia, Erythroblastic, Acute; D007942:Leukemia, Experimental / Q000378:metabolism / Q000648:ultrastructure; D013228:Stearates
D004273:DNA, Neoplasm; D005227:Fatty Acids; D005456:Fluorescent Dyes; D013228:Stearates; C023714:A(2)C
Virology. 1959 Jul;8(3):396-7
13669362
J Cell Physiol. 1972 Oct;80(2):253-9
4674183
Biochim Biophys Acta. 1974 Sep 6;363(2):261-6
4608965
Exp Cell Res. 1973 Jan;76(1):170-4
4566311
Nature. 1967 Jan 21;213(5073):261-4
6067685
J Cell Biol. 1969 Dec;43(3):618-21
5389138
Bacteriol Rev. 1973 Jun;37(2):197-214
4581783
Biol Rev Camb Philos Soc. 1971 Feb;46(1):97-155
4399534
Int Rev Cytol. 1971;31:169-213
4400359
Exp Cell Res. 1974 Nov;89(1):111-20
4373255
Cell Differ. 1975 Jun;4(3):179-85
1056810
Cell. 1975 Jul;5(3):319-22
1056809
Proc Natl Acad Sci U S A. 1971 Feb;68(2):378-82
5277089
Nature. 1976 Jul 29;262(5567):353-6
785272
Biochim Biophys Acta. 1975 Sep 2;401(3):458-67
810163
Am Sci. 1976 May-Jun;64(3):279-90
776052
Proc Natl Acad Sci U S A. 1976 Mar;73(3):862-6
1062798
Proc Natl Acad Sci U S A. 1975 Mar;72(3):869-73
1055386
Biochim Biophys Acta. 1975 Sep 2;401(3):530-4
1182150
Cell. 1975 Jun;5(2):123-9
1170022