title: Microphotometrical quantification of protein thiols in morphologically intact cells of the cervical epithelium. I. Basic results with superficial cells of healthy women.
abstract: The investigations are based on Esterbauer's results according to which the staining of protein thiols (PSH) with DDD-Fast Blue B (Barrnett and Seligman) is of a quantitative order. Consequently, the PSH of the nucleus and the cytoplasm of superficial cells of the ectocervical epithelium of healthy women are determined to 0.3 and 4.1 X 10(-14) moles, respectively. After it has been found that the mean absorption spectrum between 310 and 238 nm of the pure proteins, the extinction at 278 nm of a cytoplasmic area (300 to 400 micron2) of unstained cells is taken as a first measure for the cytoplasmic protein content which is calculated to approximately 5 X 10(-18) moles per micron2 whereas the PSH content results to 1.9 X 10(-17) moles per micron2. From this value results a very plausible number of 3.6 SH per 10(5) g of cytoplasmic proteins.
mesh_terms: D002467:Cell Nucleus / Q000032:analysis; D002584:Cervix Uteri / Q000032:analysis* / Q000166:cytology; D003593:Cytoplasm / Q000032:analysis; D004847:Epithelial Cells; D004848:Epithelium / Q000032:analysis; D005260:Female; D006651:Histocytochemistry; D006801:Humans; D010783:Photometry; D011506:Proteins / Q000032:analysis*; D013438:Sulfhydryl Compounds / Q000032:analysis*
chemical_list: D011506:Proteins; D013438:Sulfhydryl Compounds