The role of macrophages in stimulation of immune induction and myelopoiesis: II: analysis of genetic restriction involved in the stimulation of granulocyte colony precursors or mature lymphocytes using factors prepared from different recombinant inbred strains of mice.
The genetic restriction involved in the reconstitution of immune responses in macrophage-depleted mouse spleen cultures, or the induction of colony formation in bone marrow cultures, by different molecular-weight species of lymphostimulatory molecules derived from mouse peritoneal cell suspensions is reported. The data suggest little evidence for genetic restriction in the ability of any of the factors to stimulate bone marrow colony formation in vitro. However, when immunological stimulation was investigated, a restriction coded for by genes in the K/D end of the MHC (70-90K factor) or in the I region of the MHC (30-45K factor) was observed. A third species of lymphostimulatory molecule (15K) showed no such restriction. Further evidence is presented to suggest that the active moiety in the 70-90K molecule(s) is a 15K-like species (nonrestricted in its ability to reconstitute cells from different strains of mice.
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D006098:Granulocytes / Q000166:cytology*; D006183:H-2 Antigens / Q000235:genetics* / Q000276:immunology; D006410:Hematopoiesis / Q000187:drug effects; D008214:Lymphocytes / Q000166:cytology; D008222:Lymphokines / Q000494:pharmacology*; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics* / Q000276:immunology
D006183:H-2 Antigens; D008222:Lymphokines