title: Selective loss of wheat germ agglutinin binding to agglutinin-resistant mutants of Chinese hamster ovary cells.
abstract: The binding of 125I-labeled wheat germ agglutinin (WGA) to parental and three distinct WGA-resistant Chinese hamster ovary cell lines possessing modified cell surface carbohydrate structures has been examined over a 10(6)-fold range of WGA concentrations. The Scatchard plot for WGA binding to parental cells was complex and exhibited positively cooperative binding at the high affinity sites. One of the WGA-resistant mutants (WgaRIII) was apparently not altered in its WGA-binding ability compared with parental cells. However, two of the WGA-resistant lines (WgaRI and WgaRII) had distinct alterations in their WGA-binding properties specific to certain regions of the binding curve. Neither appeared to be affected in either the highest or lowest affinity regions of the binding curve. Thus, lectin-resistant cell mutants altered in specific lectin-binding sites at the cell surface provide a direct approach to analysis of the complex binding parameters that characterize the interaction of WGA with the plasma membrane.
mesh_terms: D002460:Cell Line; D037102:Lectins / Q000378:metabolism*; D009154:Mutation
chemical_list: D037102:Lectins
references.citation: J Biol Chem. 1977 Feb 10;252(3):1107-16
references.pmid: 320201
references.citation: J Biol Chem. 1977 Jun 10;252(11):3926-33
references.pmid: 558978
references.citation: Somatic Cell Genet. 1977 Jul;3(4):391-405
references.pmid: 601679
references.citation: Biochim Biophys Acta. 1976 Sep 7;443(3):587-600
references.pmid: 963070
references.citation: Adv Protein Chem. 1973;27:349-467
references.pmid: 4272599
references.citation: Int Rev Cytol. 1974;39:89-190
references.pmid: 4611947
references.citation: J Biol Chem. 1976 Apr 10;251(7):1877-88
references.pmid: 5434
references.citation: Biochim Biophys Acta. 1975 May 6;389(2):401-6
references.pmid: 166678
references.citation: J Biol Chem. 1974 May 25;249(10):3116-22
references.pmid: 4830237
references.citation: Biochemistry. 1973 Mar 27;12(7):1312-23
references.pmid: 4696755
references.citation: Biochemistry. 1976 Jul 13;15(14):3015-9
references.pmid: 952846
references.citation: Proc Natl Acad Sci U S A. 1975 Sep;72(9):3323-7
references.pmid: 1059116
references.citation: Eur J Biochem. 1976 May 17;65(1):61-9
references.pmid: 1278183
references.citation: J Biol Chem. 1976 Jul 10;251(13):4017-25
references.pmid: 932019
references.citation: Exp Cell Res. 1976 Feb;97(2):445-8
references.pmid: 942925
references.citation: Cell. 1975 Oct;6(2):121-8
references.pmid: 1182798