Bioelectricity and regeneration: large currents leave the stumps of regenerating newt limbs.
Electrical currents near regenerating newt limbs were measured with a recently developed vibrating probe. Steady currents with local surface densities of 10 to 100 muA/cm2 or more leave the end of the stump during the first 5-10 days after amputation and are balanced by currents with densities of only 1-3 muA/cm2 that enter the intact skin around the stump. They are immediately dependent upon the entry of sodium ions into this skin and are therefore inferred to be skin-driven. The outward currents are comparable in direction, density, duration, and position to artificially imposed currents previously found sufficient to induce significant regeneration of amputated adult frog limbs. This comparison suggests that the endogenous stump currents play some causal role in initiating regeneration.
D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D003714:Denervation; D004594:Electrophysiology; D004848:Epithelium / Q000502:physiology; D005121:Extremities / Q000294:innervation / Q000502:physiology*; D008239:Lysine / Q000031:analogs & derivatives / Q000494:pharmacology; D012038:Regeneration; D012455:Salamandridae / Q000502:physiology*; D012879:Skin Physiological Phenomena; D013997:Time Factors; D014562:Caudata / Q000502:physiology*
D000584:Amiloride; D008239:Lysine
J Bone Joint Surg Am. 1961 Jul;43-A:643-56
14448529
Comp Biochem Physiol. 1963 Sep;10:55-67
14109735
IRE Trans Med Electron. 1960 Jul;ME-7:202-7
13688481
J Cell Physiol. 1955 Feb;45(1):61-87
14367438
Annu Rev Biophys Bioeng. 1977;6:445-76
326151
J Exp Zool. 1977 Jun;200(3):403-16
301554
J Gen Physiol. 1977 Jun;69(6):743-63
19555
Science. 1977 Jan 21;195(4275):292-4
299785
Nature. 1977 Feb 17;265(5595):602-5
859559
Nature. 1977 Feb 17;265(5595):600-2
859558
J Cell Biol. 1974 Nov;63(2 Pt 1):614-28
4421919
Comp Biochem Physiol A Comp Physiol. 1975 Apr 1;50(4):639-43
236114
Ann N Y Acad Sci. 1974;238:500-7
4548335
J Gen Physiol. 1976 Jul;68(1):43-63
7635
Growth. 1974 SEP;38(3):363-80
4424911
Am Zool. 1970 May;10(2):191-9
4913142
Harvey Lect. 1965;59:1-30
5337820
Am Zool. 1970 Aug;10(3):365-76
5431889