Under-modified Y base in a tRHAPhe isoacceptor observed in tumor cells.
A fluorescent wye (Ye) was isolated from tRHAPhe specific to Ehrlich ascites cells. The structure was determined to be alpha-amino-beta-hydroxy-4,9,-dihydro-4,6-dimethyl-9-oxo-1-H-imidazo(1,2-alpha)purine-7-butyric acid: namely the compound lacking methyl carboxyl and methyl groups and thus is an under-modified precursor of hydroxy-Y base present in normal liver tRNAPhe.
D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000032:analysis*; D055598:Chemical Phenomena; D002621:Chemistry; D005453:Fluorescence; D006147:Guanine / Q000031:analogs & derivatives; D008099:Liver / Q000032:analysis; D051379:Mice; D009694:Nucleic Acid Precursors / Q000302:isolation & purification*; D010649:Phenylalanine; D012334:RNA, Neoplasm / Q000302:isolation & purification*; D012346:RNA, Transfer, Amino Acyl / Q000302:isolation & purification*; D051381:Rats
D009694:Nucleic Acid Precursors; D012334:RNA, Neoplasm; D012346:RNA, Transfer, Amino Acyl; C012099:wye; D010649:Phenylalanine; D006147:Guanine