title: Degradation of [3H]thymidine by a pentosyltransferase (EC 2.4.2.4) in the plasma of man and different animals.
abstract: [3H]Thymidine is degraded by an enzyme (thymidine phosphorylase; EC 2.4.2.4) which we have identified in the plasma of man and some animals. The presence of this enzyme in plasma or sera used to supplement culture media may, under certain experimental conditions, limit the validity of measuring the uptake of radiolabeled thymidine as a means of defining DNA synthesis.
mesh_terms: D000818:Animals; D004247:DNA / Q000096:biosynthesis; D005260:Female; D006801:Humans; D007700:Kinetics; D007951:Leukemia, Myeloid / Q000201:enzymology; D008297:Male; D010430:Pentosyltransferases / Q000097:blood*; D013045:Species Specificity; D013936:Thymidine / Q000097:blood*; D013939:Thymidine Phosphorylase / Q000097:blood*
chemical_list: D004247:DNA; D010430:Pentosyltransferases; D013939:Thymidine Phosphorylase; D013936:Thymidine
references.citation: J Biol Chem. 1954 Mar;207(1):245-56
references.pmid: 13152099
references.citation: J Biol Chem. 1968 Jun 10;243(11):2871-5
references.pmid: 5653178
references.citation: J Biol Chem. 1968 Apr 10;243(7):1649-55
references.pmid: 5647278
references.citation: J Biol Chem. 1964 Aug;239:2618-21
references.pmid: 14235544
references.citation: J Biol Chem. 1967 Nov 10;242(21):5059-68
references.pmid: 6058946
references.citation: J Clin Invest. 1964 Feb;43:267-78
references.pmid: 14162535
references.citation: Proc Soc Exp Biol Med. 1964 Jan;115:51-4
references.pmid: 14117468
references.citation: Br J Cancer. 1964 Dec;18:701-13
references.pmid: 14264935
references.citation: J Biol Chem. 1975 May 25;250(10):3660-5
references.pmid: 236298
references.citation: Cancer Res. 1976 Aug;36(8):2780-4
references.pmid: 1064467