Formation of 1-beta-D-arabinofuranosylcytosine diphosphate choline in neoplastic and normal cells.
1-beta-D-Arabinofuranosylcytosine diphosphate choline was formed from 1-beta-D-arabinofuranosylcytosine (ara-C) during incubation in vitro of peripheral myeloblasts from patients with acute myelogenous leukemia and cultured cells (nonleukemic human lymphocytes, mouse lymphoma L5178Y, and HeLa); as well, 1-beta-D-arabinofuranosylcytosine diphosphate choline was formed in vivo in mouse leukemia L1210 cells and mouse liver. 3-Deazauridine enhanced the anabolism of ara-C in nonleukemic lymphocytes in vitro and leukemia L1210 cells in vivo but did not influence ara-C anabolism in the other cell types. In acute myelogenous leukemia myeloblasts incubated in vitro with ara-C, concentrations of 1-beta-D-arabinofuranosylcytosine 5'-triphosphate were maximal after 8 hr of incubation and formation of the latter preceded that of 1-beta-D-arabinofuranosylcytosine diphosphate choline.
D015092:3-Deazauridine / Q000494:pharmacology; D000818:Animals; D002794:Choline / Q000031:analogs & derivatives*; D003561:Cytarabine / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000378:metabolism / Q000494:pharmacology; D003566:Cytidine Diphosphate Choline / Q000031:analogs & derivatives* / Q000096:biosynthesis; D004357:Drug Synergism; D005260:Female; D006367:HeLa Cells / Q000378:metabolism; D007939:Leukemia L1210 / Q000378:metabolism; D007942:Leukemia, Experimental / Q000188:drug therapy / Q000378:metabolism*; D015470:Leukemia, Myeloid, Acute / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008214:Lymphocytes / Q000378:metabolism; D051379:Mice; D008815:Mice, Inbred Strains
D003561:Cytarabine; D015092:3-Deazauridine; D003566:Cytidine Diphosphate Choline; D002794:Choline