Terminal deoxynucleotidyltransferase distribution in neoplastic and hematopoietic cells.
In the present study, terminal deoxynucleotidyltransferase was examined in the peripheral blood and (or) bone marrow of 115 children with a variety of neoplastic, hematologic, and other unrelated disorders. Terminal deoxynucleotidyltransferase activity was present at 4.08+/-0.74 U/108 cells in 23 morphologicall normal bone marrow samples from childhood controls. Terminal transferase was present at greater than 23 U/108 nucleated cells and at greater than31 U/108 blasts in the bone marrow of all children with acute lymphoblastic leukemia studied at initial diagnosis and at disease relapse. Terminal deoxynucleotidyltransferase was detectable at low levels, less than 7.5 U/108 cells, in all remission marrow smaples. Bone marrow terminal transferase activity was markedly elevated in all untreated acute lymphoblastic leukemia patients, whereas low levels which were difficult to interpret were present in the peripheral blood samples of two patients at diagnosis and six patients at relapse who had low absolute lymphoblast counts. Because of greater variation in the lymphoblast content of peripheral blood, bone marrow assays are more reliable in detecting disease activity. Marrow terminal deoxynucleotidyltransferase values obtained during the active phase of acute lymphoblastic leukemia were significantly greater than those found in other types of leukemia, bone marrow malignancies, and hematologic disorders. Terminal transferase determinations in blast cells of two patients with leukemic conversion of non-Hodgkin's lymphoma and in tumor cells from one patient with Burkitt's lymphoma were within the control range. These dat further define the usefulness of terminal deoxynucleotidyltrnasferase assay in the differentiation and classication of hematologic malignancies.
D000293:Adolescent; D000741:Anemia, Aplastic / Q000201:enzymology; D001853:Bone Marrow / Q000201:enzymology*; D001854:Bone Marrow Cells; D002648:Child; D002675:Child, Preschool; D004254:DNA Nucleotidyltransferases / Q000032:analysis* / Q000097:blood; D006801:Humans; D007945:Leukemia, Lymphoid / Q000201:enzymology*; D015470:Leukemia, Myeloid, Acute / Q000201:enzymology; D008214:Lymphocytes / Q000201:enzymology*; D008223:Lymphoma / Q000201:enzymology; D009369:Neoplasms / Q000201:enzymology*; D009447:Neuroblastoma / Q000201:enzymology; D009841:Oligonucleotides; D011696:Purpura, Thrombocytopenic / Q000201:enzymology; D012008:Recurrence; D012075:Remission, Spontaneous; D012208:Rhabdomyosarcoma / Q000201:enzymology
D009841:Oligonucleotides; D004254:DNA Nucleotidyltransferases
Br J Haematol. 1976 Nov;34(3):447-56
62584
Scand J Clin Lab Invest Suppl. 1968;97:77-89
4179068
Nature. 1974 Mar 29;248(447):409-11
4545077
Biochem Biophys Res Commun. 1971 Jul 2;44(1):124-31
4330042
Proc Natl Acad Sci U S A. 1973 Feb;70(2):521-5
4346893
Biochem Biophys Res Commun. 1973 Apr 2;51(3):529-35
4512981
Immunology. 1970 Oct;19(4):637-50
4097588
N Engl J Med. 1976 Jan 29;294(5):245-8
53789
Lancet. 1976 Mar 6;1(7958):508-9
55782
J Clin Invest. 1973 Apr;52(4):919-28
4571426
Br Med J. 1974 Jun 22;2(5920):643-6
4546214
Clin Immunol Immunopathol. 1973 Jul;1(4):511-22
4549205
Biochem Biophys Res Commun. 1975 Jul 22;65(2):673-82
238537
J Biol Chem. 1974 Dec 25;249(24):8051-3
4529878
Proc Natl Acad Sci U S A. 1974 Nov;71(11):4404-8
4530991
Proc Natl Acad Sci U S A. 1975 Oct;72(10):4119-22
812089
N Engl J Med. 1975 Apr 17;292(16):828-32
1078713
Blood. 1976 Jan;47(1):11-20
1081893
Cancer Res. 1976 Jan;36(1):120-7
1061638
N Engl J Med. 1975 Apr 10;292(15):775-80
1054106
Biochem Biophys Res Commun. 1975 Jan 20;62(2):367-75
1053892
J Biol Chem. 1976 Apr 25;251(8):2399-404
1262329
Science. 1975 Oct 17;190(4211):284-5
1179209
Res Commun Chem Pathol Pharmacol. 1975 Apr;10(4):715-24
1153847
J Exp Med. 1975 Apr 1;141(4):855-65
1127379
J Biol Chem. 1971 Feb 25;246(4):909-16
5543689
Biochemistry. 1971 Feb 2;10(3):536-42
5543985