Effect of uridine on cellular UTP and glycogen synthesis in skeletal muscle: stimulation of UTP formation by insulin.
The relation between cellular uracil nucleotides and ability to synthesize glycogen was studied in rat diaphragm incubated in vitro. In the absence of exogenous uridine the tissue content of UTP and rate of glycogen synthesis decreased with time. Uridine added to the medium increased cellular UTP and UDPG and stimulated glycogen synthesis. Insulin significantly increased the synthesis of UTP from extracellular uridine. This action of insulin appeared to be due to a stimulation of phosphorylation of the nucleoside and not to an effect on transport at the concentrations of uridine studied. However, an effect of insulin on transport of uridine at low concentrations cannot be excluded.
D000818:Animals; D001693:Biological Transport, Active; D003964:Diaphragm / Q000187:drug effects / Q000378:metabolism; D006003:Glycogen / Q000096:biosynthesis*; D007328:Insulin / Q000494:pharmacology*; D007700:Kinetics; D008297:Male; D009132:Muscles / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D014500:Uracil Nucleotides / Q000378:metabolism*; D014529:Uridine / Q000378:metabolism / Q000494:pharmacology*; D014532:Uridine Diphosphate Glucose / Q000378:metabolism
D007328:Insulin; D014500:Uracil Nucleotides; D006003:Glycogen; D014532:Uridine Diphosphate Glucose; D014529:Uridine
Biochim Biophys Acta. 1960 Mar 25;39:171-3
13842294
J Biol Chem. 1970 May 25;245(10):2722-9
5464041
Nature. 1963 Sep 21;199:1183-4
14072040
Arch Biochem Biophys. 1961 Sep;94:436-42
13781408
J Pharmacol Exp Ther. 1976 Dec;199(3):565-74
186586
Am J Physiol. 1968 Apr;214(4):825-31
5642943
Anal Biochem. 1968 Oct 24;25(1):486-99
5704765
Biochem J. 1971 Oct;124(5):943-7
4331862
Anal Biochem. 1970 Nov;38(1):105-14
4320192
Biochem Pharmacol. 1974 Jun 15;23(12):1675-85
4210202
J Clin Pathol. 1974 Oct;27(10):789-93
4214838
Pediatr Res. 1974 Jul;8(7):724-34
4601347
Biochem Pharmacol. 1976 Feb 15;25(4):439-45
181002
Biochim Biophys Acta. 1974 Jan 23;337(1):41-9
4433545
J Biol Chem. 1976 Apr 10;251(7):1913-9
818081
Eur J Biochem. 1975 Mar 3;52(1):117-23
809265
Am J Physiol. 1975 May;228(5):1531-4
1130556
Life Sci. 1975 Apr 1;16(7):1121-32
1134186
Biochim Biophys Acta. 1964 Nov 15;91:380-6
14254009