Chemical modification as a probe of conformational changes in transfer ribonucleic acid on aminoacylation.
Treatment of Escherichia coli CA265 phenylalanyl-tRNA with 3M-NaHSO3, pH6.0, at 25 degrees C resulted in modification of four bases and in the deacylation of the charged tRNAphe. The similarity of the rates of base modification and of the deacylation of the phenylalanyl-tRNA permitted the isolation of partially modified phenylalanyl-tRNAphe and partially modified deacylated tRNAphe. The sites and extents of base modification in these fractions were determined and found to be the same as those in uncharged tRNAphe modified under identical conditions. These findings are discussed in relation to previous evidence for and against a conformational change in tRNA on its aminoacylation. The methods described should prove adaptable to study of other aminoacyl-tRNA species.
D055598:Chemical Phenomena; D002621:Chemistry; D009690:Nucleic Acid Conformation / Q000187:drug effects*; D009843:Oligoribonucleotides / Q000032:analysis; D012346:RNA, Transfer, Amino Acyl; D013447:Sulfites / Q000494:pharmacology
D009843:Oligoribonucleotides; D012346:RNA, Transfer, Amino Acyl; D013447:Sulfites
J Biol Chem. 1972 Jun 25;247(12):3864-7
4338231
Biochemistry. 1976 Jun 1;15(11):2484-90
776221
Biochem Biophys Res Commun. 1974 Feb 4;56(3):807-14
4826879
Science. 1967 Dec 29;158(3809):1695-9
6059650
Science. 1976 Nov 19;194(4267):796-806
790568
Nucleic Acids Res. 1976 Dec;3(12):3383-96
794838
Biochem Biophys Res Commun. 1965 Aug 16;20(4):400-5
5324054
Biochemistry. 1973 Feb 27;12(5):905-14
4568769
Biochemistry. 1970 Apr 14;9(8):1694-700
4909079
Biochem Biophys Res Commun. 1968 Aug 13;32(3):507-11
5666735
J Mol Biol. 1976 Dec 25;108(4):619-49
798036
Nature. 1970 May 30;226(5248):817-20
5444622
J Mol Biol. 1965 Sep;13(2):373-98
5325727
FEBS Lett. 1969 Jun;3(4):275-278
11947028
Biochemistry. 1970 Jul 7;9(14):2858-65
5459538
J Biol Chem. 1966 Nov 25;241(22):5391-400
5333665
Biochemistry. 1976 Jul 13;15(14):3031-9
782513
FEBS Lett. 1970 Sep 7;9(6):327-330
11947706
Biochem Biophys Res Commun. 1972 Nov 1;49(3):635-40
4565123
Nature. 1968 Dec 28;220(5174):1304-7
4882917
Eur J Biochem. 1977 Jan 3;72(1):117-25
318996
Proc Natl Acad Sci U S A. 1973 Aug;70(8):2193-5
4599618
J Biol Chem. 1976 Mar 10;251(5):1529-30
767336
J Biol Chem. 1967 Mar 10;242(5):1060-4
5335910
Biochim Biophys Acta. 1970 Mar 19;204(1):183-90
4908645
Nucleic Acids Res. 1975 Jun;2(6):853-64
1096084
Biochem Biophys Res Commun. 1973 Sep 18;54(2):570-7
4585688
Biochem Biophys Res Commun. 1971 Oct 15;45(2):488-94
4946276
Eur J Biochem. 1971 Feb;18(4):496-502
5545006
J Mol Biol. 1969 Jan 14;39(1):199-217
4329286
J Mol Biol. 1972 Apr 28;66(1):131-42
4339187
J Mol Biol. 1972 Jan 14;63(1):153-69
4552761
J Mol Biol. 1972 Nov 14;71(2):217-29
4564479