title: In vitro inhibition of protein synthesis by purified cores from vaccinia virus.
abstract: The mechanism of the shutoff of cellular protein synthesis in vaccinia virus-infected cells has been investigated by using in vitro systems. Purified vaccinia cores cause inhibition of endogenous mRNA translation in nonpreincubated reticulocyte lysates and Ehrlich ascites tumor cell-free systems. Translation of viral mRNA from turnip yellow mosaic virus is also impaired in wheat germ cell-free extracts. The block induced by vaccinia cores in protein synthesis is not due to a decrease in the availability of mRNA but rather to an alteration of the cellular translational machinery. No nucleolytic activity able of digesting mRNA could be detected in purified vaccinia cores with three sensitive tests. There is a lack of inhibition in the poly(Phe)-poly(U) system, which bypasses the normal initiation process. An almost complete disaggregation of polyribosomes in the reticulocyte lysate appears when vaccinia cores are present. These results indicate that mRNA translation in a cell-free system is affected predominantly at the level of polypeptide chain initiation.
mesh_terms: D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism; D002474:Cell-Free System; D010442:Peptide Chain Initiation, Translational; D011072:Poly U / Q000378:metabolism; D011132:Polyribosomes / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D012156:Reticulocytes / Q000378:metabolism; D012260:Ribonucleases / Q000378:metabolism; D014908:Triticum; D014616:Vaccinia virus / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D011072:Poly U; D012260:Ribonucleases
references.citation: J Mol Biol. 1964 Feb;8:263-76
references.pmid: 14126295
references.citation: Nature. 1963 Jul 27;199:357-8
references.pmid: 14058568
references.citation: J Cell Biol. 1963 Jul;18:51-72
references.pmid: 14024720
references.citation: Biochem Biophys Res Commun. 1976 Nov 8;73(1):149-56
references.pmid: 999695
references.citation: Biochem Biophys Res Commun. 1976 SEP 20;72(2):768-75
references.pmid: 985647
references.citation: J Gen Virol. 1973 May;19(2):201-6
references.pmid: 4268458
references.citation: J Biol Chem. 1974 May 25;249(10):3281-6
references.pmid: 4364422
references.citation: J Biol Chem. 1974 May 25;249(10):3273-80
references.pmid: 4364421
references.citation: Proc Natl Acad Sci U S A. 1968 Jul;60(3):845-52
references.pmid: 4299268
references.citation: Proc Natl Acad Sci U S A. 1971 May;68(5):1075-8
references.pmid: 4325000
references.citation: J Virol. 1975 Jun;15(6):1467-74
references.pmid: 1170340
references.citation: J Virol. 1973 Dec;12(6):1434-41
references.pmid: 4586778
references.citation: Prog Med Virol. 1975;19:40-83
references.pmid: 164054
references.citation: Anal Biochem. 1973 May;53(1):304-8
references.pmid: 4711106
references.citation: J Virol. 1972 Sep;10(3):417-24
references.pmid: 5072434
references.citation: J Virol. 1968 Mar;2(3):167-72
references.pmid: 4986904
references.citation: Nature. 1970 Aug 29;227(5261):913-8
references.pmid: 4915406
references.citation: Nature. 1976 Jan 15;259(5539):150-1
references.pmid: 1246353
references.citation: J Biol Chem. 1975 Apr 10;250(7):2420-9
references.pmid: 1123320
references.citation: J Virol. 1975 Jul;16(1):34-42
references.pmid: 1133878
references.citation: J Virol. 1968 Oct;2(10):1028-37
references.pmid: 5723706
references.citation: J Ultrastruct Res. 1966 Mar;14(5):484-96
references.pmid: 5930347