Manual fluorometry of phenylalanine from blood specimens collected on filter paper: a modified procedure.
In this manual fluorometric method, blood samples are used that have been impregnated on the filter paper, a convenient collection technique that is widely used to screen newborns for phenylketonuria. The modified procedure, based on the method of McCaman and Robins [J. Lab Clin. Med. 59, 885 (1962)], includes elution of phenylalanine from specimens on filter paper, removal of proteins by precipitation with trichloroacetic acid at 0 degrees C, and then reaction with ninhydrin-peptide reagent for color development. The standard curve is linear to at least 200 mg/L and the CV is 6.3% for a phenylalanine concentration of 27 mg/L. The modified procedure is suitable both for early screening for phenylketonuria and for monitoring blood phenylalanine of phenylketonurics during dietary therapy.
D001800:Blood Specimen Collection; D019411:Clinical Laboratory Techniques; D006801:Humans; D007231:Infant, Newborn; D008403:Mass Screening; D010209:Paper; D010649:Phenylalanine / Q000097:blood*; D010661:Phenylketonurias / Q000175:diagnosis*; D013050:Spectrometry, Fluorescence / Q000379:methods
D010649:Phenylalanine