title: Construction and characterization of an Escherichia coli plasmid bearing a functional gene G of bacteriophage phiX174.
abstract: In order to study the mutagenic effects of site-specific, covalent modifications of biologically active DNA, we need host cells that are permissive for any type of mutation that might be produced in vivo from the modified DNA. Specifically, we require a general, in vivo complementation system for the bacteriophage phiX174 gene G, an essential gene that we have chosen for our initial studies of chemical mutagenesis. Toward this end, we have constructed a plasmid (pphiXG) that carries a functional copy of phiX174 gene G. Three different bacterial strains that are nonpermissive for am9, a gene G amber mutant, have been transformed with pphiXG. The transformants are now permissive for this gene G mutant, but not for the gene A or E mutants that have been tested. This paper describes the construction and the biochemical characterization of this plasmid, pphiXG, and describes some of the biological properties exhibited by the pphiXG-bearing strains.
mesh_terms: D003090:Coliphages / Q000235:genetics*; D004269:DNA, Bacterial / Q000378:metabolism*; D004274:DNA, Recombinant; D005814:Genes, Viral; D009154:Mutation; D010957:Plasmids; D014170:Transformation, Genetic
chemical_list: D004269:DNA, Bacterial; D004274:DNA, Recombinant
references.citation: Cell. 1976 Jun;8(2):163-82
references.pmid: 61066
references.citation: Proc Natl Acad Sci U S A. 1976 Sep;73(9):3146-50
references.pmid: 61588
references.citation: Nucleic Acids Res. 1976 Jan;3(1):101-16
references.pmid: 765970
references.citation: J Mol Biol. 1976 Jun 5;103(4):699-710
references.pmid: 781283
references.citation: Bacteriol Rev. 1976 Dec;40(4):869-907
references.pmid: 795416
references.citation: Nature. 1977 Feb 24;265(5596):687-95
references.pmid: 870828
references.citation: J Mol Biol. 1977 May 15;112(2):265-77
references.pmid: 875019
references.citation: J Mol Biol. 1976 Dec 25;108(4):771-9
references.pmid: 1018324
references.citation: Proc Natl Acad Sci U S A. 1975 Dec;72(12):5135-9
references.pmid: 1061098
references.citation: Nucleic Acids Res. 1976 Aug;3(8):1947-60
references.pmid: 1085927
references.citation: CRC Crit Rev Microbiol. 1975 Dec;4(2):161-223
references.pmid: 1106952
references.citation: Cell. 1974 Dec;3(4):315-25
references.pmid: 4216403
references.citation: J Bacteriol. 1972 May;110(2):667-76
references.pmid: 4336693
references.citation: Proc Natl Acad Sci U S A. 1972 Oct;69(10):2904-9
references.pmid: 4342968
references.citation: Proc Natl Acad Sci U S A. 1974 Jul;71(7):2882-6
references.pmid: 4527604
references.citation: Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110-4
references.pmid: 4559594
references.citation: J Virol. 1974 Apr;13(4):898-907
references.pmid: 4595301
references.citation: Virology. 1974 Nov;62(1):270-5
references.pmid: 4608028
references.citation: J Mol Biol. 1971 Jun 28;58(3):739-53
references.pmid: 4933426
references.citation: J Biol Chem. 1967 Feb 25;242(4):672-8
references.pmid: 6017736
references.citation: J Biol Chem. 1959 Oct;234:2733-4
references.pmid: 13802337
references.citation: J Virol. 1971 May;7(5):549-58
references.pmid: 16789129
references.citation: J Virol. 1972 Jul;10(1):99-114
references.pmid: 16789133