Magnetic properties of oxyhemoglobin.
When the magnetic susceptibility of frozen aqueous solutions of human oxyhemoglobin was measured in the range between 25 and 250 K, it showed a temperature-dependent behavior typical of a thermal equilibrium between a ground singlet state and an excited triplet state for two electrons per heme, the energy separation being [2J] = 146 cm-1. By contrast, within the same temperature range, carboxyhemoglobin was found to be diamagnetic, as already reported.
D002263:Carboxyhemoglobin; D006801:Humans; D008280:Magnetics; D010108:Oxyhemoglobins; D011487:Protein Conformation; D013696:Temperature; D013816:Thermodynamics
D010108:Oxyhemoglobins; D002263:Carboxyhemoglobin
Proc Natl Acad Sci U S A. 1936 Apr;22(4):210-6
16577697
Nature. 1964 Apr 4;202:83-4
14166723
J Am Chem Soc. 1974 Aug 21;96(17):5599-5600
4854122
Nature. 1970 Nov 21;228(5273):726-39
5528785
J Am Chem Soc. 1974 Jan 23;96(2):338-45
4361043
Proc Natl Acad Sci U S A. 1970 Dec;67(4):1846-53
4321348
Biochem Biophys Res Commun. 1973 Nov 1;55(1):91-6
4787738
Proc Natl Acad Sci U S A. 1975 Jun;72(6):2335-9
1056005
Proc Natl Acad Sci U S A. 1968 Feb;59(2):526-32
5238982
Biochim Biophys Acta. 1969 Dec 23;194(2):351-63
5366907
J Mol Biol. 1966 Jul;18(3):385-404
5966294