title: Translation in vitro of rat liver messenger RNA coding for ligandin (glutathione S-transferase B).
abstract: Poly(A)-containing rat liver mRNA isolated from animals injected with phenobarbital and uninjected controls was translated efficiently in a wheat-germ system. The synthesis of ligandin (glutathione S-transferase B; glutathione transferase; RX-gluathione R-transferase, EC 2.5.1.18) was detected by immunoprecipitation with a highly purified monospecific ligandin antibody and analysis by sodium dodecyl sulfate/polyacrylamide gel electrophoresis. The extent of incorporation of [35S]methionine into ligandin in the translation system was similar for poly(A)-containing messages from un-infected animals and those treated with phenobarbital.
mesh_terms: D000818:Animals; D002474:Cell-Free System; D005982:Glutathione Transferase / Q000096:biosynthesis*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008970:Molecular Weight; D010634:Phenobarbital / Q000494:pharmacology; D011061:Poly A / Q000302:isolation & purification; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D014908:Triticum
chemical_list: D012333:RNA, Messenger; D011061:Poly A; D005982:Glutathione Transferase; D010634:Phenobarbital
references.citation: Prog Allergy. 1958;5:1-78
references.pmid: 13578996
references.citation: Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12
references.pmid: 4504350
references.citation: Proc Natl Acad Sci U S A. 1973 Aug;70(8):2330-4
references.pmid: 4525168
references.citation: Proc Natl Acad Sci U S A. 1974 Oct;71(10):3879-82
references.pmid: 4139704
references.citation: Int J Cancer. 1973 Mar 15;11(2):398-411
references.pmid: 4364264
references.citation: J Clin Invest. 1972 Mar;51(3):677-84
references.pmid: 4622106
references.citation: J Biol Chem. 1974 Nov 25;249(22):7130-9
references.pmid: 4436300
references.citation: Eur J Biochem. 1974 Jul 1;46(1):83-8
references.pmid: 4850204
references.citation: J Biol Chem. 1973 Nov 25;248(22):7661-8
references.pmid: 4750421
references.citation: Biochem Biophys Res Commun. 1970 Mar 27;38(6):1141-8
references.pmid: 4985441
references.citation: J Clin Invest. 1969 Nov;48(11):2156-67
references.pmid: 4980931
references.citation: Biochem J. 1967 May;103(2):316-24
references.pmid: 4962081
references.citation: Nature. 1971 Dec 24;234(5330):466-7
references.pmid: 4944188
references.citation: Annu Rev Biochem. 1976;45:747-803
references.pmid: 786161
references.citation: Proc Natl Acad Sci U S A. 1969 Sep;64(1):168-70
references.pmid: 5262999
references.citation: J Clin Invest. 1971 Nov;50(11):2242-52
references.pmid: 5096510
references.citation: Biochemistry. 1969 Dec;8(12):4813-21
references.pmid: 5365784
references.citation: J Biol Chem. 1969 Jun 25;244(12):3303-15
references.pmid: 5792661