A quick method for concentrating and processing cancer cells from serous fluids and fine-needle nodule aspirates.
A quick method of concentrating cancer cells and the preparation of cytological slides from body fluids and aspirates is described, using a single Ficoll gradient and cytocentrifuge. The method eliminates the disadvantages of conventional techniques, i.e., excess contamination by red (erythrocytes) or white (leukocytes) blood cells and a scarcity of cancer cells. Because the technique is simple and requires only standard cytotechnological equipment, it can be easily adopted as an aid to diagnostic routine in cancer cytology.
D001202:Ascitic Fluid / Q000166:cytology; D001707:Biopsy, Needle; D001826:Body Fluids / Q000166:cytology*; D001854:Bone Marrow Cells; D002469:Cell Separation; D002555:Cerebrospinal Fluid / Q000166:cytology; D006801:Humans; D009369:Neoplasms / Q000473:pathology*; D010996:Pleural Effusion / Q000166:cytology; D013048:Specimen Handling / Q000379:methods*