title: Transfer of codominant markers by isolated metaphase chromosomes in Chinese hamster ovary cells.
abstract: Codominant mutations to methotrexate and ouabain resistance in Chinese hamster ovary cells can be transferred to recipient Chinese hamster ovary cells by isolated metaphase chromosomes. For methotrexate, both the structural change and the increased activity of dihydrofolate reductase (5,6,7,8-tetrahydrofolate:NADP+ oxidoreductase EC 1.5.1.3), characteristic of the donor cells, are observed in the transferents (cells that carry and express functions derived by chromosome transfer). The transferents are unstable in the absence of selection although stable clones can be isolated. From results obtained by fractionation of chromosomes and transfer to recipients, the methotrexate and ouabain markers can be assigned to the middle and large size-classes of chromosomes, respectively. By fractionation and transfer of chromosomes, from transferents to new recipients, evidence has been obtained that chromosome integration is not restricted to a particular chromosomal site in the recipient.
mesh_terms: D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D002875:Chromosomes / Q000187:drug effects / Q000502:physiology*; D004351:Drug Resistance; D005799:Genes, Dominant; D008677:Metaphase / Q000187:drug effects; D008727:Methotrexate / Q000494:pharmacology; D009154:Mutation; D010042:Ouabain / Q000494:pharmacology; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics
chemical_list: D010042:Ouabain; D013762:Tetrahydrofolate Dehydrogenase; D008727:Methotrexate
references.citation: Can J Biochem. 1977 Apr;55(4):445-52
references.pmid: 15711
references.citation: Exp Cell Res. 1976 Nov;103(1):15-22
references.pmid: 186287
references.citation: Cell. 1976 Oct;9(2):269-87
references.pmid: 184963
references.citation: In Vitro. 1976 Nov;12(11):749-57
references.pmid: 1035580
references.citation: Somatic Cell Genet. 1976 May;2(3):245-61
references.pmid: 1028172
references.citation: Cell. 1976 Oct;9(2):213-9
references.pmid: 975243
references.citation: Somatic Cell Genet. 1975 Apr;1(2):137-51
references.pmid: 1235904
references.citation: Proc Natl Acad Sci U S A. 1976 Apr;73(4):1274-8
references.pmid: 177984
references.citation: Proc Natl Acad Sci U S A. 1972 Nov;69(11):3119-23
references.pmid: 4508306
references.citation: Proc Natl Acad Sci U S A. 1973 Apr;70(4):1258-62
references.pmid: 4515624
references.citation: Nat New Biol. 1971 Mar 10;230(10):52-4
references.pmid: 5279808
references.citation: Virology. 1974 Sep;61(1):303-5
references.pmid: 4369749
references.citation: Cell. 1975 Feb;4(2):167-72
references.pmid: 1168545
references.citation: J Mol Biol. 1968 Feb 28;32(1):101-12
references.pmid: 4230571
references.citation: Proc Natl Acad Sci U S A. 1975 May;72(5):1792-6
references.pmid: 1057170
references.citation: Cell. 1974 Jul;2(3):173-82
references.pmid: 4547236
references.citation: Proc Natl Acad Sci U S A. 1976 Aug;73(8):2838-42
references.pmid: 1066695
references.citation: Proc Natl Acad Sci U S A. 1975 May;72(5):1797-801
references.pmid: 1057770
references.citation: Cell. 1975 Oct;6(2):121-8
references.pmid: 1182798
references.citation: Exp Cell Res. 1970 Mar;59(3):469-78
references.pmid: 5461579