title: Increase of proliferative activity of leukaemic blast cells from human peripheral blood in liquid culture.
abstract: The proliferative behaviour of leukaemic cells from the peripheral blood of 12 patients suffering from acute leukaemia was investigated in short term liquid culture. In 3 cases of AML an increase of the blast cell number was observed exceeding the initial value whereas in 9 other cases the cell number decreased more or less rapidly. In 9 of these patients the proliferation kinetics of the cultured leukaemic blast cells were studied with 3H-thymidine labelling. In all these cases the labelling index increased during the first days of culture, in 3 cases to values of 48%, 45% and 38% on day 3. Only in 5 cases, however, did an absolute increase of the blast cells incorporating 3H-thymidine occur. Here the doubling times of the proliferating leukaemic blast cells were estimated to be 12, 13, 14, 28 and 55 h. Since a doubling time of 12 to 14 h seems to be too short to be explained only by an exponential growth of the initially proliferating cells it is postulated that leukaemic blast cells in a G0- or long G1-phase were present in the peripheral blood of these patients and that these entered the cell cycle during liquid culture.
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002455:Cell Division; D002478:Cells, Cultured; D003470:Culture Media; D005260:Female; D006801:Humans; D007938:Leukemia / Q000097:blood*; D007945:Leukemia, Lymphoid / Q000097:blood; D007948:Leukemia, Monocytic, Acute / Q000097:blood; D015470:Leukemia, Myeloid, Acute / Q000097:blood; D007958:Leukocyte Count; D007962:Leukocytes; D008214:Lymphocytes; D008297:Male; D008875:Middle Aged; D009000:Monocytes
chemical_list: D003470:Culture Media