MRNA-directed synthesis of catalytically active mouse beta-glucuronidase in Xenopus oocytes.
Catalytically active mouse beta-glucuronidase (beta-D-glucuronide glucuronosohydrolase, EC 3.2.1.31) is formed when Xenopus oocytes are injected with mouse RNA enriched for poly(A)-containing mRNA sequences. With the RNA from androgen-induced kidneys, the efficiency of translation is comparable to that of endogenous Xenopus messenger, and the fidelity of translation is high. Detection of glucuronidase messenger by formation of a catalytically active product is several orders of magnitude more sensitive than detection by incorporation of isotopically labeled amino acids. As well as providing a sensitive technique for examining the regulation of gene expression, the system makes available an opportunity to study the regulation of post-translational polypeptide processing of a lysosomal enzyme.
D000818:Animals; D002384:Catalysis; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005966:Glucuronidase / Q000096:biosynthesis* / Q000235:genetics; D066298:In Vitro Techniques; D051379:Mice; D008805:Mice, Inbred A; D009865:Oocytes / Q000201:enzymology*; D010063:Ovum / Q000201:enzymology*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics / Q000502:physiology*; D014981:Xenopus
D012333:RNA, Messenger; D005966:Glucuronidase
J Biol Chem. 1965 Jul;240:2811-6
14342299
Metabolism. 1964 Oct;13:SUPPL:985-1002
14228777
J Biol Chem. 1976 Dec 25;251(24):7753-60
826534
Cell. 1976 Dec;9(4 PT 2):761-74
1017013
J Biol Chem. 1969 Nov 25;244(22):6168-76
4900511
Arch Biochem Biophys. 1968 Jul;126(1):155-64
5671059
Biochem J. 1974 Mar;138(3):395-405
4215418
J Mol Biol. 1973 Jul 5;77(3):371-89
4727071
J Biol Chem. 1975 Nov 10;250(21):8503-9
1194264
J Biol Chem. 1975 Jun 25;250(12):4765-9
1141228
J Biol Chem. 1975 Feb 10;250(3):1146-8
1112792
J Cell Physiol. 1975 Apr;85(2 Pt 2 Suppl 1):379-92
1123410
Nature. 1970 Aug 15;227(5259):680-5
5432063
J Biol Chem. 1970 Jun;245(12):3059-65
5432796
Biochemistry. 1967 Dec;6(12):3676-81
5624598