title: Conformation of beta-endorphin and beta-lipotropin: formation of helical structure in methanol and sodium dodecyl sulfate solutions.
abstract: Circular dichroic spectra of camel beta-endorphin and ovine beta-lipotropin in water show little, if any, secondary structure. Intrinsic viscosities and sedimentation coefficients of the two peptides also suggest that the molecules are not compact and globular. Methanol or sodium dodecyl sulfate promotes the formation of helical structure to an extent as much as one-half of either peptide molecule. The conformation of the complex between camel beta-endorphin and dodecyl sulfate may be related to the opiate-like function of this peptide hormone.
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002162:Camelus; D002942:Circular Dichroism; D004723:Endorphins; D006146:Guanidines; D006801:Humans; D000432:Methanol; D010902:Pituitary Gland; D011487:Protein Conformation; D012756:Sheep; D012967:Sodium Dodecyl Sulfate; D013045:Species Specificity; D008083:beta-Lipotropin
chemical_list: D004723:Endorphins; D006146:Guanidines; D012967:Sodium Dodecyl Sulfate; D008083:beta-Lipotropin; D000432:Methanol
references.citation: Biochemistry. 1977 Apr 5;16(7):1462-6
references.pmid: 14681
references.citation: Can J Biochem. 1976 Nov;54(11):992-8
references.pmid: 11882
references.citation: J Med Chem. 1977 Mar;20(3):325-8
references.pmid: 845862
references.citation: Mol Biol (Mosk). 1976 May-Jun;10(2):704-12
references.pmid: 1053052
references.citation: J Med Chem. 1976 Nov;19(11):1354-6
references.pmid: 1003418
references.citation: Biochem Biophys Res Commun. 1976 Oct 18;72(4):1542-7
references.pmid: 999687
references.citation: Biophys J. 1976 Oct;16(10):1201-38
references.pmid: 963199
references.citation: Adv Protein Chem. 1968;23:121-282
references.pmid: 4882248
references.citation: Biochemistry. 1974 Jan 15;13(2):222-45
references.pmid: 4358940
references.citation: Biochemistry. 1974 Jan 15;13(2):211-22
references.pmid: 4358939
references.citation: Life Sci. 1974 Jun 1;14(11):2231-45
references.pmid: 4847814
references.citation: Proc Natl Acad Sci U S A. 1973 Jul;70(7):1947-9
references.pmid: 4516196
references.citation: Proc Natl Acad Sci U S A. 1971 Aug;68(8):1742-7
references.pmid: 5288759
references.citation: Nature. 1965 Dec 11;208(5015):1093-4
references.pmid: 4162144
references.citation: Life Sci. 1975 Jun 15;16(12):1811-7
references.pmid: 1152609
references.citation: Biochem Biophys Res Commun. 1976 Jul 26;71(2):558-65
references.pmid: 962939
references.citation: Nature. 1976 Aug 26;262(5571):779-82
references.pmid: 958448
references.citation: Nature. 1976 Aug 26;262(5571):778-9
references.pmid: 958447
references.citation: Biochemistry. 1974 Jul 30;13(16):3350-9
references.pmid: 4366945
references.citation: Biochemistry. 1972 Oct 24;11(22):4120-31
references.pmid: 4343790
references.citation: Biochem Biophys Res Commun. 1973 Aug 21;53(4):1304-9
references.pmid: 4270658
references.citation: Acta Pharmacol Toxicol (Copenh). 1973;32(3):317-20
references.pmid: 4801733
references.citation: Science. 1973 Mar 9;179(4077):1011-4
references.pmid: 4687585
references.citation: Proc Natl Acad Sci U S A. 1976 Apr;73(4):1145-8
references.pmid: 1063395
references.citation: Biochem Biophys Res Commun. 1976 Jul 12;71(1):19-25
references.pmid: 962912
references.citation: Nature. 1975 Dec 18;258(5536):577-80
references.pmid: 1207728
references.citation: J Am Chem Soc. 1970 Apr 8;92(7):2119-29
references.pmid: 5435277
references.citation: J Am Chem Soc. 1969 Jan 1;91(1):184-90
references.pmid: 5782812