title: Regional dissociation of beta-endorphin and enkephalin contents in rat brain and pituitary.
abstract: beta-Endorphin and enkephalin in extracts of whole brain, various brain regions, adenohypophysis, and combined pars intermedia and neurohypophysis of the rat were measured by radioimmunoassay. In brain extracts, the immunoreactive substances were further separated according to molecular size by gel filtration. beta-Endorphin was found in the diencephalon but not in the hippocampus, cerebral cortex, cerebellum, and striatum. Enkephalin was found predominantly in the striatum and diencephalon. Attention is called to possible artifactual interference by myelin basic protein in the immunoassays for beta-endorphin in some regions of the brain. In the pituitary, enkephalin was mainly restricted to the pars intermedia-neurohypophysis. Neither adrenalectomy nor hypophysectomy significantly altered levels of beta-endorphin in brain extracts. Adrenalectomy increased the levels of beta-endorphin in adenohypophysis and pars intermedia-neurohypophysis; after adrenalectomy, enkephalin was also increased in the adenohypophysis but less so in the pars intermedia-neurohypophysis. These results show that brain endorphin levels are independent of pituitary endorphin levels; they suggest that beta-endorphin-containing neurons and those containing enkephalin constitute two separate groups of brain cells.
mesh_terms: D000315:Adrenalectomy; D000818:Animals; D001923:Brain Chemistry; D004723:Endorphins / Q000032:analysis*; D004745:Enkephalins / Q000032:analysis*; D007016:Hypophysectomy; D008297:Male; D010902:Pituitary Gland / Q000032:analysis*; D011863:Radioimmunoassay; D051381:Rats
chemical_list: D004723:Endorphins; D004745:Enkephalins
references.citation: Proc Natl Acad Sci U S A. 1977 May;74(5):2167-71
references.pmid: 194249
references.citation: Biochem Biophys Res Commun. 1977 Jun 6;76(3):930-6
references.pmid: 197933
references.citation: Proc Natl Acad Sci U S A. 1977 Jul;74(7):3014-8
references.pmid: 197529
references.citation: Can J Biochem. 1977 Jun;55(6):666-70
references.pmid: 195688
references.citation: Life Sci. 1977 Jan 1;20(1):43-7
references.pmid: 319317
references.citation: Proc Natl Acad Sci U S A. 1977 Jun;74(6):2584-8
references.pmid: 267951
references.citation: Biochem Biophys Res Commun. 1977 Jul 11;77(1):361-6
references.pmid: 883982
references.citation: Neuropharmacology. 1977 Apr;16(4):303-7
references.pmid: 859689
references.citation: J Med Chem. 1977 Mar;20(3):325-8
references.pmid: 845862
references.citation: Proc Natl Acad Sci U S A. 1976 Sep;73(9):3308-10
references.pmid: 1067621
references.citation: J Neurochem. 1966 Aug;13(8):655-69
references.pmid: 5950056
references.citation: C R Acad Sci Hebd Seances Acad Sci D. 1976 Feb 23;282(8):783-5
references.pmid: 817843
references.citation: Proc Natl Acad Sci U S A. 1976 Jul;73(7):2515-9
references.pmid: 1065904
references.citation: Proc Natl Acad Sci U S A. 1976 Jun;73(6):2156-9
references.pmid: 1064883
references.citation: Proc Natl Acad Sci U S A. 1976 Jun;73(6):1821-3
references.pmid: 1064855
references.citation: Nature. 1976 Sep 16;263(5574):240-2
references.pmid: 958478
references.citation: Nature. 1976 Apr 29;260(5554):793-5
references.pmid: 1264258
references.citation: Nature. 1975 Dec 18;258(5536):577-80
references.pmid: 1207728
references.citation: Neuroscience. 1976 Aug;1(4):349-51
references.pmid: 11370520