Hemoglobin switching in sheep and goats: induction of hemoglobin C synthesis in cultures of sheep fetal erythroid cells.
Synthesis of HbF (alpha2gamma2) is replaced by synthesis of Hb A (alpha2betaA2) shortly before birth in sheep homozygous for the betaA globin chain whereas Hb C (alpha2betaC2) is produced transiently during the neonatal period. We have obtained a Hb F-to-Hb C switch by generating erythroid colonies at high erythropoietin concentration in plasma clot cultures of mid-gestation fetal bone marrow or liver. Furthermore, high erythropoietin concentration appeared specifically to activate the gene for betaC and not those for betaA or betaB globin in colonies grown from cells of an animal heterozygous for the betaA and betaB genes. Erythropoietic stress in the form of periodic bleeding or erythropoietin injection in utero did not stimulate production of Hb C (alpha2betaC2) in fetal sheep until shortly before birth, and then only in two of six animals. Thus, factors other than erythropoietin may influence the potential for betaC globin synthesis in vivo in fetal sheep.
D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D005319:Fetal Hemoglobin / Q000096:biosynthesis; D005333:Fetus / Q000378:metabolism*; D005865:Gestational Age; D006444:Hemoglobin C / Q000096:biosynthesis*; D008297:Male; D011247:Pregnancy; D012756:Sheep
D006444:Hemoglobin C; D005319:Fetal Hemoglobin
Science. 1977 Jun 10;196(4295):1213-4
860136
Nature. 1976 Dec 23-30;264(5588):799-801
1012326
J Cell Biol. 1976 Dec;71(3):715-26
993267
Proc Natl Acad Sci U S A. 1973 Jun;70(6):1739-43
4515932
Proc Natl Acad Sci U S A. 1972 Aug;69(8):2184-8
4506088
Proc Natl Acad Sci U S A. 1971 Jul;68(7):1542-6
4104431
Blood. 1974 Aug;44(2):285-90
4136729
J Cell Biol. 1975 Mar;64(3):515-27
1171104
Nature. 1976 Mar 4;260(5546):67-70
1264198
Ann N Y Acad Sci. 1974 Nov 29;241(0):691-8
4530689
Ann N Y Acad Sci. 1974 Nov 29;241(0):672-82
4530687
Science. 1973 Apr 20;180(4083):310-2
4735594
Proc Soc Exp Biol Med. 1972 Apr;139(4):1148-50
5023307
Proc Natl Acad Sci U S A. 1976 Jun;73(6):2033-7
1064874
Isr J Med Sci. 1971 Jul-Aug;7(7):850-6
5098373
Pediatrics. 1970 May;45(5):766-72
5462809
J Lab Clin Med. 1969 Nov;74(5):782-8
5388952
Pediatr Res. 1969 May;3(3):189-98
5788781
J Clin Invest. 1968 Aug;47(8):1895-904
5672611
Nature. 1966 Mar 12;209(5028):1099-102
5927138
Science. 1966 Sep 23;153(3743):1539-43
5917784