title: Nucleotide sequence of an insertion element, IS1.
abstract: PSM2, PSM1, and PSM15 are small plasmids derived from R100 by spontaneous deletions at either end of the insertion sequence IS1. These plasmids were used to identify regions neighboring IS1 as well as the IS1 DNA itself, by cleavage with EcoR1, HindIII, Hae III, Hpa II, Hha I, Hinf, and AIu I. The nucleotide sequencing results demonstrate that IS1 contains 768 bases. About 30 bases at the ends of IS1 were found to be repeated in an inverted order. The deletions occurring at the ends of IS1 were found to be due to illegitimate recombination. The hypothesis that RNA polymerase could play an important role in such recombination phenomena is discussed based on the nucleotide sequences surrounding the recombinational hot spots.
mesh_terms: D001483:Base Sequence; D004262:DNA Restriction Enzymes; D011815:R Factors; D011995:Recombination, Genetic
chemical_list: D004262:DNA Restriction Enzymes
references.citation: Nature. 1976 Oct 28;263(5580):731-8
references.pmid: 792710
references.citation: Proc Natl Acad Sci U S A. 1977 Sep;74(9):3932-6
references.pmid: 333450
references.citation: Plasmid. 1977 Nov;1(1):8-18
references.pmid: 375272
references.citation: Science. 1977 Sep 16;197(4309):1147-60
references.pmid: 331474
references.citation: Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4
references.pmid: 265521
references.citation: Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184-8
references.pmid: 1055375
references.citation: Nature. 1974 Aug 2;250(465):394-7
references.pmid: 4854243
references.citation: Proc Natl Acad Sci U S A. 1975 Mar;72(3):784-8
references.pmid: 1093168
references.citation: Nature. 1975 Mar 13;254(5496):118-21
references.pmid: 1117996
references.citation: Mol Gen Genet. 1973 May 9;122(3):279-86
references.pmid: 4577901
references.citation: Mol Gen Genet. 1973 May 9;122(3):267-77
references.pmid: 4577900
references.citation: Mol Gen Genet. 1972;119(3):223-31
references.pmid: 4567156
references.citation: Mol Gen Genet. 1972;119(3):191-206
references.pmid: 4567154
references.citation: J Mol Biol. 1974 Jan 5;85(4):485-500
references.pmid: 4853525
references.citation: Biochemistry. 1970 Oct 27;9(22):4428-40
references.pmid: 4919547
references.citation: Mol Gen Genet. 1967;100(2):178-89
references.pmid: 4869989
references.citation: J Bacteriol. 1975 May;122(2):764-75
references.pmid: 1092668
references.citation: Proc Natl Acad Sci U S A. 1975 Apr;72(4):1373-7
references.pmid: 1093180
references.citation: J Bacteriol. 1975 Aug;123(2):687-92
references.pmid: 1097422
references.citation: J Mol Biol. 1975 Oct 5;97(4):561-75
references.pmid: 1102715
references.citation: Proc Natl Acad Sci U S A. 1975 Sep;72(9):3623-7
references.pmid: 1103150
references.citation: Proc Natl Acad Sci U S A. 1975 Sep;72(9):3628-32
references.pmid: 1059152
references.citation: Nature. 1975 Mar 13;254(5496):114-7
references.pmid: 1117995