General methodology for the estimation of common neutral urinary steroids by multi-columin liquid chromatography.
A method is described for the estimation of common neutral urinary steroids by multi-column liquid chromatography. Hydrolysis is performed in two steps: enzymatically, using beta-glucuronidase, followed by solvolysis. Initial short column chromatography separates the neutral steroids into three fractions according to polarity: 17-oxosteroids, corticosteriods less polar than cortolones, and cortolones and cortols. The cortolone, cortol fraction is oxidized and the different steroid groups are chromatographed on capillary aluminum oxide and silica gel columns. A computerized, spectrophotometric system is used for the quantitation procedure.
D015068:17-Ketosteroids / Q000652:urine; D000305:Adrenal Cortex Hormones / Q000652:urine; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D003201:Computers; D005069:Evaluation Studies as Topic; D005966:Glucuronidase; D006801:Humans; D006868:Hydrolysis; D013053:Spectrophotometry; D013256:Steroids / Q000652:urine*
D015068:17-Ketosteroids; D000305:Adrenal Cortex Hormones; D013256:Steroids; D005966:Glucuronidase