title: Human somatotropin: restoration of full biological activity by noncovalent interaction of a natural and a synthetic fragment of the hormone.
abstract: The recombinant hormone obtained by noncovalent interaction of the natural NH2-terminal fragment (consisting of 134 amino acid residues) with a synthetic COOH-terminal fragment of 52 amino acids of the reduced-carbamidomethylated human somatotropin molecule is found to exhibit full biological activity of the native hormone as evidenced by the tibia test. Radioimmunoassay data indicate that the semisynthetic recombinant hormone possesses essentially full immunoreactivity as compared to the native one. In addition, circular dichroism and fluorescence emission spectra of the semisynthetic recombinant are identical to that of the undisociated, plasmin modified, reduced-carbamidomethylated hormone.
mesh_terms: D001681:Biological Assay; D002942:Circular Dichroism; D013006:Growth Hormone / Q000276:immunology / Q000502:physiology; D006801:Humans; D011487:Protein Conformation; D013050:Spectrometry, Fluorescence; D013329:Structure-Activity Relationship
chemical_list: D013006:Growth Hormone
references.citation: Endocrinology. 1949 Nov;45(5):455-63, illust
references.pmid: 15396704
references.citation: Biochem J. 1963 Oct;89:114-23
references.pmid: 14097352
references.citation: Arch Biochem Biophys. 1962 Sep;Suppl 1:327-32
references.pmid: 13930384
references.citation: Proc Natl Acad Sci U S A. 1976 Oct;73(10):3463-5
references.pmid: 62361
references.citation: Proc Natl Acad Sci U S A. 1974 Apr;71(4):1197-201
references.pmid: 4275394
references.citation: Arch Biochem Biophys. 1974 Oct;164(2):571-4
references.pmid: 4282220
references.citation: Proc Natl Acad Sci U S A. 1976 May;73(5):1476-9
references.pmid: 58417
references.citation: Arch Biochem Biophys. 1975 Mar;167(1):80-90
references.pmid: 236733
references.citation: Biochemistry. 1972 Oct 24;11(22):4179-87
references.pmid: 5086543
references.citation: Proc Natl Acad Sci U S A. 1975 Oct;72(10):3878-82
references.pmid: 812085
references.citation: Int J Pept Protein Res. 1975;7(6):495-501
references.pmid: 1201912
references.citation: Biochemistry. 1969 Dec;8(12):4701-8
references.pmid: 5365779
references.citation: Endocrinology. 1966 Aug;79(2):275-80
references.pmid: 5921766