title: Succinyl concanavalin A stimulates and antimicrotubular drugs inhibit the synthesis of a brain-specific protein in rat glial cells.
abstract: The relative synthesis of the brain-specific s100 protein increased as clonal rat glial cells, C6, progressed from logarithmic to stationary growth in monolayer culture. Drugs that disrupt microtubules, such as colchicine, vinblastine, Colcemid, and podophyllotoxin, inhibited the relative synthesis of S100 protein in stationary cultures. Colchicine (0.1 muM) caused a 50% inhibition of the relative synthesis of S100 protein whereas lumicolchicine,an isomer of colchicine that does not disrupt the microtubular system, had no effect. Succinylated concanavalin A (500 mug/ml) increased relative synthesis in logarithmic but not stationary cultures. These results suggest that signals inducing an increase in the relative synthesis of S100 protein in stationary cultures are transmitted intracellularly from the cell membrane by the microtubular network.
mesh_terms: D002460:Cell Line; D003078:Colchicine / Q000494:pharmacology*; D003208:Concanavalin A / Q000031:analogs & derivatives* / Q000494:pharmacology; D003703:Demecolcine / Q000494:pharmacology; D006118:Griseofulvin / Q000494:pharmacology; D007700:Kinetics; D008870:Microtubules; D008940:Mitotic Index / Q000187:drug effects; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D009457:Neuroglia / Q000378:metabolism; D011034:Podophyllotoxin / Q000494:pharmacology; D009418:S100 Proteins / Q000096:biosynthesis*; D014747:Vinblastine / Q000494:pharmacology
chemical_list: D009419:Nerve Tissue Proteins; D009418:S100 Proteins; D003208:Concanavalin A; D006118:Griseofulvin; D014747:Vinblastine; D011034:Podophyllotoxin; D003078:Colchicine; D003703:Demecolcine
references.citation: Biochem J. 1956 Feb;62(2):315-23
references.pmid: 13293190
references.citation: Proc Soc Exp Biol Med. 1964 Dec;117:899-901
references.pmid: 14244985
references.citation: Exp Cell Res. 1962 Aug;27:307-16
references.pmid: 14456559
references.citation: Proc Natl Acad Sci U S A. 1973 Apr;70(4):1012-6
references.pmid: 4515602
references.citation: Proc Natl Acad Sci U S A. 1973 May;70(5):1442-6
references.pmid: 4514314
references.citation: Biochim Biophys Acta. 1976 Apr 30;458(1):1-72
references.pmid: 181093
references.citation: Biochim Biophys Acta. 1975 Jul 11;417(2):153-72
references.pmid: 167866
references.citation: J Cell Physiol. 1970 Jun;75(3):329-39
references.pmid: 4988781
references.citation: Proc Natl Acad Sci U S A. 1976 Jul;73(7):2375-8
references.pmid: 1065889
references.citation: J Mol Biol. 1976 Apr 25;102(4):817-29
references.pmid: 781264
references.citation: Science. 1976 Apr 16;192(4236):218-26
references.pmid: 769162
references.citation: Nature. 1976 Aug 26;262(5571):785-6
references.pmid: 958451
references.citation: Nature. 1976 May 27;261(5558):318-9
references.pmid: 1272405
references.citation: Biochim Biophys Acta. 1976 Apr 13;457(1):57-108
references.pmid: 1260065
references.citation: Biochem Biophys Res Commun. 1976 Jan 26;68(2):401-8
references.pmid: 943164
references.citation: Eur J Biochem. 1975 Oct 1;58(1):73-9
references.pmid: 1237401
references.citation: Nature. 1975 Jul 3;256(5512):19-22
references.pmid: 1169693
references.citation: J Cell Biol. 1975 Jul;66(1):128-44
references.pmid: 1170172
references.citation: Proc Natl Acad Sci U S A. 1970 May;66(1):160-7
references.pmid: 5273894
references.citation: J Cell Physiol. 1971 Aug;78(1):145-52
references.pmid: 5165084