title: Chromosomal map location of the methicillin resistance determinant in Staphylococcus aureus.
abstract: Three-factor genetic crosses performed by transformation have shown that the methicillin resistance determinant of Staphylococcus aureus strain DU4916 (the mec-4916 marker) is linked to a novobiocin resistance (Novr) marker (nov-142) and mutational sites affecting pyrimidine (pyr-141), purine (pur-102), and histidine (hisG15) biosynthesis in S. aureus strain 8325. The linkage group thus defined is pyr-141-hisG15-nov-142-pur-102-mec-4916. Phage 80alpha previously propagated on a novobiocin-resistant, methicillin-sensitive (Mecs) 8325 strain was used to infect 21 novobiocin-sensitive, methicillin-resistant clinical isolates (including strain DU4916). Among the novobiocin-resistant transductants so obtained from each recipient, between 1 and 5% were methicillin sensitive (reflecting cotransduction of Novr and Mecs). These results are consistent with the genetic determinant of methicillin resistance having a single chromosomal locus in most, if not all, strains of S. aureus.
mesh_terms: D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D005796:Genes; D008040:Genetic Linkage; D008712:Methicillin / Q000494:pharmacology*; D010403:Penicillin Resistance; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics*; D014161:Transduction, Genetic; D014169:Transformation, Bacterial
chemical_list: D008712:Methicillin
references.citation: J Gen Microbiol. 1965 May;39:195-207
references.pmid: 14324965
references.citation: J Gen Microbiol. 1964 May;35:183-90
references.pmid: 14179667
references.citation: J Gen Microbiol. 1963 Oct;33:121-36
references.pmid: 14072829
references.citation: Plasmid. 1977 Nov;1(1):38-51
references.pmid: 618184
references.citation: J Bacteriol. 1977 Feb;129(2):778-88
references.pmid: 138674
references.citation: J Bacteriol. 1977 Jan;129(1):237-45
references.pmid: 137229
references.citation: Bacteriol Rev. 1977 Dec;41(4):872-902
references.pmid: 339893
references.citation: J Bacteriol. 1977 Jan;129(1):317-25
references.pmid: 830641
references.citation: Pathology. 1976 Jan;8(1):69-72
references.pmid: 972766
references.citation: J Bacteriol. 1973 Feb;113(2):576-85
references.pmid: 4266172
references.citation: J Bacteriol. 1973 Nov;116(2):803-11
references.pmid: 4270449
references.citation: Genetics. 1974 Apr;76(4):681-702
references.pmid: 4275652
references.citation: J Bacteriol. 1974 Apr;118(1):155-64
references.pmid: 4274456
references.citation: J Gen Microbiol. 1974 Sep;84(1):1-10
references.pmid: 4497533
references.citation: J Med Microbiol. 1972 Nov;5(4):497-508
references.pmid: 4486061
references.citation: J Gen Microbiol. 1973 May;76(1):1-11
references.pmid: 4489832
references.citation: J Med Microbiol. 1973 Nov;6(4):511-26
references.pmid: 4491549
references.citation: Antimicrob Agents Chemother. 1972 Sep;2(3):217-23
references.pmid: 4494517
references.citation: J Bacteriol. 1973 Nov;116(2):771-7
references.pmid: 4490525
references.citation: J Bacteriol. 1975 Sep;123(3):905-15
references.pmid: 125746
references.citation: J Bacteriol. 1976 Sep;127(3):1167-72
references.pmid: 956123
references.citation: J Med Microbiol. 1976 May;9(2):137-48
references.pmid: 1047111
references.citation: J Bacteriol. 1975 Oct;124(1):201-11
references.pmid: 1176430