title: Vasoactive intestinal polypeptide in brain: localization in and release from isolated nerve terminals.
abstract: The vasoactive intestinal polypeptide was present in synaptosomal (nerve ending) preparations from cerebral cortex, hypothalamus, and striatum of rat brain in higher concentrations than in these tissues as a whole. The total content and relative specific activity of the peptide increased with progressive purification of the synaptosomal fractions and generally followed the distribution of known synaptosomal constituents--dopamine, norepinephrine, and lactate dehydrogenase (L-lactate:NAD+ oxidoreductase, EC 1.1.1.27). The peptide was also released from synaptosomal pellets with increased K+ concentration, and this release was Ca2+-dependent. The findings suggest a role for vasoactive intestinal polypeptide as a transmitter or modulator of synaptic function.
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D002540:Cerebral Cortex / Q000378:metabolism; D003342:Corpus Striatum / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism; D005768:Gastrointestinal Hormones / Q000378:metabolism*; D007031:Hypothalamus / Q000378:metabolism; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D013347:Subcellular Fractions / Q000378:metabolism; D013385:Succinate Dehydrogenase / Q000378:metabolism; D013574:Synaptosomes / Q000378:metabolism* / Q000648:ultrastructure; D014018:Tissue Distribution; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism*
chemical_list: D005768:Gastrointestinal Hormones; D014660:Vasoactive Intestinal Peptide; D013385:Succinate Dehydrogenase; D004298:Dopamine
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