Liposome--lymphocyte interaction: saturable sites for transfer and intracellular release of liposome contents.
The water-soluble dye 6-carboxyfluorescein was trapped in the internal aqueous compartments of small sonicated dioleoyl lecithin vesicles and used to assess the kinetics of transfer of vesicle contents to human lymphocytes. By using flow microfluorometry, the initial rate of dye transfer to the cells was measured as a function of the concentration of vesicles in the external medium. The rate of transfer consists of at least two components, one of which saturates at high vesicle concentration and the other of which does not saturate in the range of concentrations explored. The saturable component was competitively inhibited by vesicles not containing dye. Both the saturable and nonsaturable components of transfer were inhibited by fetal calf serum or bovine serum albumin but neither component was affected by bovine IgG, choline chloride, or heparin. Pretreatment of the lymphocytes with trypsin or Pronase had no effect on either component. The saturable component can be interpreted in terms of a two-step process in which vesicles bind reversely to sites on the cell surface, and dye is then transferred into the cell from the vesicle-site complex.
D001769:Blood; D002459:Cell Fusion; D003470:Culture Media; D005456:Fluorescent Dyes; D006801:Humans; D007700:Kinetics; D008081:Liposomes; D008214:Lymphocytes / Q000187:drug effects; D010447:Peptide Hydrolases / Q000378:metabolism
D003470:Culture Media; D005456:Fluorescent Dyes; D008081:Liposomes; D010447:Peptide Hydrolases
Science. 1977 Feb 4;195(4277):489-92
835007
Biochim Biophys Acta. 1977 Mar 29;497(1):183-91
849477
Biochim Biophys Acta. 1976 Dec 14;457(3-4):259-302
793635
J Cell Biol. 1977 Aug;74(2):531-46
407233
Annu Rev Biochem. 1977;46:669-722
332066
Biochim Biophys Acta. 1973 Dec 28;300(4):421-65
4360422
J Cell Biol. 1974 Nov;63(2 Pt 1):492-504
4138544
J Cell Biol. 1975 Sep;66(3):621-34
1174130
J Cell Biol. 1975 Oct;67(1):49-60
1176535
Proc Natl Acad Sci U S A. 1976 May;73(5):1603-7
818640
N Engl J Med. 1976 Sep 30;295(14):765-70
785256
J Cell Biol. 1976 Sep;70(3):515-26
60342
Nature. 1975 Jan 17;253(5488):194-5
1110768
J Exp Med. 1974 Aug 1;140(2):508-22
4603014
J Exp Med. 1974 Jun 1;139(6):1553-67
4598019
Proc Natl Acad Sci U S A. 1973 Apr;70(4):1238-40
4515622
Cell. 1976 Jan;7(1):85-95
181140
Science. 1976 Jan 16;191(4223):150-4
174194
Biochemistry. 1973 Oct 23;12(22):4507-17
4796045