title: Control of antibody heterogeneity in strain 2 guinea pigs.
abstract: When Wright's strain 2 guinea pigs are immunized with 2,4-dinitrophenyl conjugates in complete Freund's adjuvant, the antibody response varies with the choice of carrier. Immunization with 2,4-dinitrophenyl-guinea pig albumin elicits a response that requires approximately 21 days to detect. The antibody produced is, according to isoelectric focusing, relatively homogeneous IgG2 having a neutral isoelectric point. On small amounts of IgG are produced. Stimulation of animals with 2,4-dinitrophenyl-keyhole Limpet hemocyanin produces a response by 14 days is similar to the peak response inititated by 2,4-dinitrophenyl-guinea pig albumin. With time, however, basic IgG2 populations are added to the response. By days 28-35, when the anti-hapten response has reached a plateau, the major subpopulation of antibody is neutral IgG2, but there exists several times as much basic IgG2 as IgG1. These data suggest that antigen-responsive cells may be ordered into groups having different thresholds of activation. Regardless of the strength of the antigenic stimulus, there is a set sequence of activation. The same cells always play the primary role, contributing most of the antibody regardless of whether secondary clones of cells are activated.
mesh_terms: D000818:Animals; D000917:Antibody Formation; D004136:Dinitrobenzenes / Q000276:immunology; D005796:Genes; D006168:Guinea Pigs; D006433:Hemocyanins / Q000276:immunology; D007074:Immunoglobulin G / Q000096:biosynthesis; D007518:Isoantibodies / Q000235:genetics*; D007525:Isoelectric Focusing; D012709:Serum Albumin / Q000276:immunology; D013997:Time Factors
chemical_list: D004136:Dinitrobenzenes; D007074:Immunoglobulin G; D007518:Isoantibodies; D012709:Serum Albumin; D006433:Hemocyanins
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