title: Production of autoinhibitory factors by mouse lymphoma cells in vitro and its relationship to thiol dependence.
abstract: Five different kinds of mouse lymphoma cells conditioned culture medium so that it became inhibitory to thiol-dependent cells but not to thiol-independent cells. Conditioning was rapid and appeared to be complete in 1-3 hr. The inhibitory effects could be reversed by concentrations of 2-mercaptoethanol higher than those usually required for growth promotion. Inhibitory factors were concentrated by ultrafiltration and chromatography on Sephadex G-10 which showed their apparent molecular weights to be 300-600. The factors were stable at 100 degrees and to proteases but were inactivated (after reduction) by iodoacetamide, indicating that they may be thiols or disulfides.
mesh_terms: D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002478:Cells, Cultured; D003470:Culture Media; D004220:Disulfides / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D007939:Leukemia L1210 / Q000503:physiopathology*; D008264:Macrophages / Q000502:physiology*; D008970:Molecular Weight; D013438:Sulfhydryl Compounds / Q000494:pharmacology*
chemical_list: D003470:Culture Media; D004220:Disulfides; D013438:Sulfhydryl Compounds
references.citation: Br J Cancer. 1976 Oct;34(4):359-67
references.pmid: 987795
references.citation: Science. 1977 May 20;196(4292):896-7
references.pmid: 140460
references.citation: Nature. 1977 Jun 16;267(5612):623-5
references.pmid: 141620
references.citation: Proc Soc Exp Biol Med. 1977 Oct;156(1):177-80
references.pmid: 410034
references.citation: Cell Immunol. 1977 Oct;33(2):233-44
references.pmid: 334378
references.citation: J Exp Med. 1975 Oct 1;142(4):887-902
references.pmid: 170358
references.citation: J Exp Med. 1973 Sep 1;138(3):574-92
references.pmid: 4727914
references.citation: J Natl Cancer Inst. 1972 Aug;49(2):579-81
references.pmid: 4672864
references.citation: Cell Immunol. 1972 Jan;3(1):156-60
references.pmid: 5061825
references.citation: J Immunol. 1976 Mar;116(3):635-8
references.pmid: 1082889
references.citation: Cancer Res. 1976 Oct;36(10):3784-8
references.pmid: 1066184
references.citation: Acta Neuropathol Suppl. 1975;Suppl 6:41-5
references.pmid: 1057363