Construction and characterization of an Escherichia coli plasmid bearing a functional gene G of bacteriophage phiX174.
In order to study the mutagenic effects of site-specific, covalent modifications of biologically active DNA, we need host cells that are permissive for any type of mutation that might be produced in vivo from the modified DNA. Specifically, we require a general, in vivo complementation system for the bacteriophage phiX174 gene G, an essential gene that we have chosen for our initial studies of chemical mutagenesis. Toward this end, we have constructed a plasmid (pphiXG) that carries a functional copy of phiX174 gene G. Three different bacterial strains that are nonpermissive for am9, a gene G amber mutant, have been transformed with pphiXG. The transformants are now permissive for this gene G mutant, but not for the gene A or E mutants that have been tested. This paper describes the construction and the biochemical characterization of this plasmid, pphiXG, and describes some of the biological properties exhibited by the pphiXG-bearing strains.
D003090:Coliphages / Q000235:genetics*; D004269:DNA, Bacterial / Q000378:metabolism*; D004274:DNA, Recombinant; D005814:Genes, Viral; D009154:Mutation; D010957:Plasmids; D014170:Transformation, Genetic
D004269:DNA, Bacterial; D004274:DNA, Recombinant
Cell. 1976 Jun;8(2):163-82
61066
Proc Natl Acad Sci U S A. 1976 Sep;73(9):3146-50
61588
Nucleic Acids Res. 1976 Jan;3(1):101-16
765970
J Mol Biol. 1976 Jun 5;103(4):699-710
781283
Bacteriol Rev. 1976 Dec;40(4):869-907
795416
Nature. 1977 Feb 24;265(5596):687-95
870828
J Mol Biol. 1977 May 15;112(2):265-77
875019
J Mol Biol. 1976 Dec 25;108(4):771-9
1018324
Proc Natl Acad Sci U S A. 1975 Dec;72(12):5135-9
1061098
Nucleic Acids Res. 1976 Aug;3(8):1947-60
1085927
CRC Crit Rev Microbiol. 1975 Dec;4(2):161-223
1106952
Cell. 1974 Dec;3(4):315-25
4216403
J Bacteriol. 1972 May;110(2):667-76
4336693
Proc Natl Acad Sci U S A. 1972 Oct;69(10):2904-9
4342968
Proc Natl Acad Sci U S A. 1974 Jul;71(7):2882-6
4527604
Proc Natl Acad Sci U S A. 1972 Aug;69(8):2110-4
4559594
J Virol. 1974 Apr;13(4):898-907
4595301
Virology. 1974 Nov;62(1):270-5
4608028
J Mol Biol. 1971 Jun 28;58(3):739-53
4933426
J Biol Chem. 1967 Feb 25;242(4):672-8
6017736
J Biol Chem. 1959 Oct;234:2733-4
13802337
J Virol. 1971 May;7(5):549-58
16789129
J Virol. 1972 Jul;10(1):99-114
16789133