Isolation of transcriptionally active chromatin from mammalian nucleoli.
Nucleoli isolated from HeLa cells are functionally active but contain large amounts of RNA and proteins (RNA/DNA ratio 1:1; protein/DNA ratio 7:1). We have isolated from the nucleolus a DNA-protein complex that has the characteristics of nucleolar chromatin (RNA/DNA ratio less than 0.05:1; protein/DNA ratio 1.7:1). This nucleolar chromatin has most of the transcriptional activity of the intact nucleolus and, as assayed by circular dichroism and dye binding, has largely preserved its structure. The isolation of a transcriptionally active, fragment of chromatin, which constitutes only a small part of the total genome and codes for only one recognizable product, offers several advantages for the study of chromatin structure and function.
D002466:Cell Nucleolus / Q000032:analysis* / Q000201:enzymology / Q000378:metabolism; D002843:Chromatin / Q000302:isolation & purification* / Q000378:metabolism / Q000648:ultrastructure; D002942:Circular Dichroism; D006367:HeLa Cells; D012260:Ribonucleases / Q000378:metabolism; D013698:Templates, Genetic; D014158:Transcription, Genetic
D002843:Chromatin; D012260:Ribonucleases
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