title: Selective scattering spectra as an approach to internal structure of granal and agranal chloroplasts.
abstract: Selective scattering spectra of granal and agranal chloroplasts were measured in the red spectral region and compared with calculations based on the Mie theory. The spectra were influenced considerably by the intactness and ultrastructural pattern of the chloroplasts. It was demonstrated that the spectra consist of two components: one attributable to grana and the other, to single lamellae. The dependence of the selective scattering spectra on the ultrastructural characteristics offers a convenient method for monitoring the quality of chloroplast preparations by a procedure much faster than electron microscopy.
mesh_terms: D002734:Chlorophyll / Q000032:analysis; D002736:Chloroplasts / Q000648:ultrastructure*; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D008433:Mathematics; D010944:Plants; D012542:Scattering, Radiation; D013053:Spectrophotometry; D003313:Zea mays
chemical_list: D002734:Chlorophyll
references.citation: Proc Natl Acad Sci U S A. 1975 Oct;72(10):3858-62
references.pmid: 669
references.citation: Bibl Laeger. 1966 Mar 14;112(3):403-21
references.pmid: 4380390
references.citation: Bull Acad Pol Sci Biol. 1974;22(11):761-8
references.pmid: 4455360
references.citation: Nat New Biol. 1972 Jan 26;235(56):125-6
references.pmid: 4501197
references.citation: Biophys J. 1972 Jul;12(7):764-73
references.pmid: 4556610
references.citation: Arch Biochem Biophys. 1969 Dec;135(1):97-108
references.pmid: 4903244
references.citation: J Theor Biol. 1968 Dec;21(3):348-67
references.pmid: 5719249
references.citation: Arch Biochem Biophys. 1961 Jan;92:1-12
references.pmid: 13692507
references.citation: Arch Biochem Biophys. 1959 Oct;84:428-41
references.pmid: 14414452
references.citation: Arch Biochem Biophys. 1962 Aug;98:274-85
references.pmid: 14462682
references.citation: Plant Physiol. 1973 Jul;52(1):54-6
references.pmid: 16658498