Development of electrophysiological and biochemical membrane properties during differentiation of embryonic skeletal muscle in culture.
Newly fused chick myotubes undergo simultaneous and rapid changes in cell membrane properties during synchronous differentiation in culture. These changes are coordinately regulated and include increases in acetylcholine receptor, acetylcholinesterase, and resting potential, as well as the appearance of action potentials in discrete membrane areas upon stimulation. Subsequently, the acetylcholine receptor reaches maximal levels, whereas the development of electrical properties is marked by a further increase in resting potential, changes in the characteristics of the elicited action potential, and the recruitment of additional membrane areas for action potential generation. Maturation of electrical excitability, marked by the acquisition of the ability to fire repetitively and to conduct action potentials along the membrane, occurs well after resting potential has reached a maximum. During post-maturational development, myotubes exhibit spontaneous electrical and contractile activity, and levels of acetylcholine receptor accessible to externally applied 125I-labeled alpha-bungarotoxin decrease markedly. It is suggested that electrophysiological membrane maturation is autonomously regulated with no requirement for neuronal intervention and involves the coordinated biosynthesis of discrete membrane components and their subsequent organization in the myotube membrane.
D000200:Action Potentials; D000818:Animals; D002038:Bungarotoxins / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D002642:Chick Embryo; D008564:Membrane Potentials / Q000187:drug effects; D009119:Muscle Contraction; D009132:Muscles / Q000196:embryology / Q000378:metabolism / Q000502:physiology*; D011950:Receptors, Cholinergic / Q000378:metabolism; D013997:Time Factors
D002038:Bungarotoxins; D011950:Receptors, Cholinergic
Dev Biol. 1977 Jun;57(2):317-29
873051
Proc Natl Acad Sci U S A. 1974 Aug;71(8):3208-11
4528510
Curr Top Dev Biol. 1969;4:37-77
4331967
Nat New Biol. 1973 Nov 28;246(152):124-6
4518939
J Physiol. 1975 Jan;244(1):145-59
1168257
J Physiol. 1975 Jan;244(1):129-43
1168256
Int Rev Neurobiol. 1972;15:83-166
4347729
Physiol Rev. 1975 Jan;55(1):1-61
162810
Science. 1974 Jul 5;185(4145):76-8
4836086
Eur J Pharmacol. 1974 Jun;27(1):5-13
4859268
Dev Biol. 1973 Mar;31(1):147-62
4787184
Science. 1973 Jul 6;181(4094):76-8
4736607
J Cell Biol. 1973 Oct;59(1):241-5
4752407
Fed Proc. 1973 Jun;32(6):1636-42
4710875
J Cell Physiol. 1972 Jun;79(3):363-6
5064752
Science. 1972 May 5;176(4034):514-6
5032352
Proc Natl Acad Sci U S A. 1976 Apr;73(4):1355-9
1063416
Prog Neurobiol. 1976;3(2):103-36
778916
Cell. 1976 Apr;7(4):543-50
954086
Proc Natl Acad Sci U S A. 1976 Jan;73(1):161-4
1061111
Proc Natl Acad Sci U S A. 1975 Apr;72(4):1368-72
1055412
Nature. 1976 May 27;261(5558):328-30
1272409
Dev Biol. 1976 Apr;49(2):412-24
1269815
Pflugers Arch. 1976 Feb 24;361(3):255-61
943769
J Cell Physiol. 1975 Dec;86(3 Pt 1):503-10
1202030
J Cell Physiol. 1971 Oct;78(2):289-99
5167851
J Exp Zool. 1970 Jul;174(3):281-6
5424683
Anal Biochem. 1966 Jul;16(1):59-64
5966131