title: MRNA-directed synthesis of catalytically active mouse beta-glucuronidase in Xenopus oocytes.
abstract: Catalytically active mouse beta-glucuronidase (beta-D-glucuronide glucuronosohydrolase, EC 3.2.1.31) is formed when Xenopus oocytes are injected with mouse RNA enriched for poly(A)-containing mRNA sequences. With the RNA from androgen-induced kidneys, the efficiency of translation is comparable to that of endogenous Xenopus messenger, and the fidelity of translation is high. Detection of glucuronidase messenger by formation of a catalytically active product is several orders of magnitude more sensitive than detection by incorporation of isotopically labeled amino acids. As well as providing a sensitive technique for examining the regulation of gene expression, the system makes available an opportunity to study the regulation of post-translational polypeptide processing of a lysosomal enzyme.
mesh_terms: D000818:Animals; D002384:Catalysis; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005966:Glucuronidase / Q000096:biosynthesis* / Q000235:genetics; D066298:In Vitro Techniques; D051379:Mice; D008805:Mice, Inbred A; D009865:Oocytes / Q000201:enzymology*; D010063:Ovum / Q000201:enzymology*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics / Q000502:physiology*; D014981:Xenopus
chemical_list: D012333:RNA, Messenger; D005966:Glucuronidase
references.citation: J Biol Chem. 1965 Jul;240:2811-6
references.pmid: 14342299
references.citation: Metabolism. 1964 Oct;13:SUPPL:985-1002
references.pmid: 14228777
references.citation: J Biol Chem. 1976 Dec 25;251(24):7753-60
references.pmid: 826534
references.citation: Cell. 1976 Dec;9(4 PT 2):761-74
references.pmid: 1017013
references.citation: J Biol Chem. 1969 Nov 25;244(22):6168-76
references.pmid: 4900511
references.citation: Arch Biochem Biophys. 1968 Jul;126(1):155-64
references.pmid: 5671059
references.citation: Biochem J. 1974 Mar;138(3):395-405
references.pmid: 4215418
references.citation: J Mol Biol. 1973 Jul 5;77(3):371-89
references.pmid: 4727071
references.citation: J Biol Chem. 1975 Nov 10;250(21):8503-9
references.pmid: 1194264
references.citation: J Biol Chem. 1975 Jun 25;250(12):4765-9
references.pmid: 1141228
references.citation: J Biol Chem. 1975 Feb 10;250(3):1146-8
references.pmid: 1112792
references.citation: J Cell Physiol. 1975 Apr;85(2 Pt 2 Suppl 1):379-92
references.pmid: 1123410
references.citation: Nature. 1970 Aug 15;227(5259):680-5
references.pmid: 5432063
references.citation: J Biol Chem. 1970 Jun;245(12):3059-65
references.pmid: 5432796
references.citation: Biochemistry. 1967 Dec;6(12):3676-81
references.pmid: 5624598