title: Physical properties of chemically acetylated rat liver chromatin.
abstract: The physical properties of rat liver chromatin and nucleosomes acetylated with acetic anhydride were examined in order to clarify the mechanism by which chemical acetylation of histones increases template activity in vitro [Marushige, K. (1976) Proc. Natl. Acad. Sci. USA 73, 3937-3941]. Acetylation was found to have dramatic effects on the magnesium solubility, nuclease sensitivity, thermal denaturation, and sedimentation of chromatin and nucleosomes. The significance of the results to models of gene activation and chromatin replication is considered.
mesh_terms: D000107:Acetylation; D000818:Animals; D002467:Cell Nucleus / Q000032:analysis; D002843:Chromatin / Q000032:analysis; D003851:Deoxyribonucleases / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000032:analysis*; D008274:Magnesium; D009691:Nucleic Acid Denaturation; D011489:Protein Denaturation; D051381:Rats; D012995:Solubility
chemical_list: D002843:Chromatin; D003851:Deoxyribonucleases; D008274:Magnesium
references.citation: Proc Natl Acad Sci U S A. 1964 May;51:786-94
references.pmid: 14172992
references.citation: J Mol Biol. 1964 Jan;8:54-64
references.pmid: 14149964
references.citation: Proc Natl Acad Sci U S A. 1976 Nov;73(11):3937-41
references.pmid: 1069278
references.citation: Proc Natl Acad Sci U S A. 1976 Nov;73(11):3966-70
references.pmid: 1069279
references.citation: Cell. 1976 Sep;9(1):179-93
references.pmid: 987855
references.citation: Science. 1976 Oct 29;194(4264):529-32
references.pmid: 973136
references.citation: Nature. 1967 Jul 22;215(5099):360-3
references.pmid: 6058287
references.citation: Proc Natl Acad Sci U S A. 1974 Jun;71(6):2193-7
references.pmid: 4526205
references.citation: J Mol Biol. 1969 Aug 14;43(3):439-60
references.pmid: 4100471
references.citation: Science. 1976 Sep 3;193(4256):848-56
references.pmid: 948749
references.citation: J Mol Biol. 1971 Jul 28;59(2):255-72
references.pmid: 4327779
references.citation: Biochemistry. 1971 Apr 13;10(8):1471-8
references.pmid: 4325601
references.citation: Biochim Biophys Acta. 1969 May 20;182(1):46-56
references.pmid: 4307348
references.citation: Cold Spring Harb Symp Quant Biol. 1974;38:803-19
references.pmid: 4524789
references.citation: J Biol Chem. 1975 Jul 10;250(13):4856-63
references.pmid: 168194
references.citation: Biochemistry. 1973 Apr 24;12(9):1763-72
references.pmid: 4699236
references.citation: Biochemistry. 1972 Mar 14;11(6):998-1003
references.pmid: 5013821
references.citation: J Mol Biol. 1970 Mar;48(3):469-87
references.pmid: 4911812
references.citation: Proc Natl Acad Sci U S A. 1971 Dec;68(12):2941-4
references.pmid: 5289239
references.citation: Proc Natl Acad Sci U S A. 1975 Mar;72(3):1212-6
references.pmid: 1055376
references.citation: Biochemistry. 1976 May 18;15(10):2041-6
references.pmid: 1276123
references.citation: Biochem Biophys Res Commun. 1975 Sep 2;66(1):403-10
references.pmid: 1164431
references.citation: Biochemistry. 1975 Aug 26;14(17):3787-94
references.pmid: 1174504
references.citation: Science. 1975 Oct 10;190(4210):117-28
references.pmid: 1166303
references.citation: Biochemistry. 1971 Apr 13;10(8):1461-70
references.pmid: 5580664
references.citation: Nature. 1970 Sep 26;227(5265):1313-8
references.pmid: 5455134