title: The effect of diphenylhydantoin on fibroblasts in vitro.
abstract: It was found that 0.02 mM was the higher concentration of drug which is not toxic and is compatible with normal cell morphology and growth over a period of a week. Using this concentration of drug there was no consistent stimulation of cell growth. A small increase in cell number was noted, however, in some cultures during the first 24 hours of culture. DpH at a concentration of 0.02 mM in the presence of ascorbic acid decreased collagen prolyl hydroxylation in "old" W1-38 cultures but a concentration of 0.1 mM was required to decrease the values to a similar extent in "young" cultures. A concentration of 0.02 mM DpH had no effect on hydroxylation in an "old" HGF culture. It is speculated that in a responsive individual a reduction of collagen prolyl hydroxylation may play a role in the etiology of DpH-induced gingival enlargement and congenital malformations.
mesh_terms: D002460:Cell Line; D003094:Collagen / Q000378:metabolism*; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D006900:Hydroxylation; D010672:Phenytoin / Q000494:pharmacology*; D011392:Proline / Q000378:metabolism
chemical_list: D010672:Phenytoin; D003094:Collagen; D011392:Proline