In vitro biosynthesis of beta-endorphin in pituitary glands.
beta-Endorphin is a 31 amino acid polypeptide isolated from the pituitary gland of different species of animals. It has strong morphine-like activity. It is formed of amino acid residues 61-91 of beta-lipotropin. Speculation has arisen whether it is biosynthesized in situ or transformed after secretion of beta-lipotropin. The present in vitro studies show that it is found as beta-endorphin in bovine pituitary slices incubated with radioactive amino acid precursor [35S]methionine. Chemical characterization and microsequencing of the newly biosynthesized material proves its identity with isolated unlabeled beta-endorphin and shows that it has a methionine residue at its fifth position, as expected.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D066298:In Vitro Techniques; D009020:Morphine; D010452:Peptide Biosynthesis; D010455:Peptides / Q000302:isolation & purification; D010902:Pituitary Gland / Q000378:metabolism*; D014176:Protein Biosynthesis
D000596:Amino Acids; D010455:Peptides; D009020:Morphine
Nature. 1962 Jul 21;195:281-3
14491328
Can J Biochem. 1976 Nov;54(11):992-8
11882
Biochem Biophys Res Commun. 1977 Feb 21;74(4):1528-35
843377
Can J Biochem. 1977 Jan;55(1):35-40
837244
Biochem Biophys Res Commun. 1976 Oct 18;72(4):1542-7
999687
Biochem Biophys Res Commun. 1976 SEP 20;72(2):472-8
985493
Fed Proc. 1974 Oct;33(10):2105-15
4370934
Nature. 1965 Dec 11;208(5015):1093-4
4162144
Can J Biochem. 1970 Apr;48(4):511-6
4192532
Can J Biochem. 1974 May;52(5):349-58
4366173
C R Acad Sci Hebd Seances Acad Sci D. 1976 Feb 23;282(8):783-5
817843
Proc Natl Acad Sci U S A. 1976 Apr;73(4):1145-8
1063395
Proc Natl Acad Sci U S A. 1976 Jun;73(6):2156-9
1064883
Proc Natl Acad Sci U S A. 1976 Jun;73(6):1821-3
1064855
FEBS Lett. 1976 Jul 15;66(2):243-5
955087
Nature. 1976 Apr 29;260(5554):793-5
1264258
Can J Biochem. 1976 Jun;54(6):566-70
1276982
Nature. 1975 Dec 18;258(5536):577-80
1207728
Biochemistry. 1975 Jul;14(13):3029-35
1148190
Science. 1968 Jul 12;161(3837):165-7
5657063
Can J Biochem. 1967 Jul;45(7):1163-74
6035976