Lipoprotein lipase cofactor activity of a carboxyl-terminal peptide of apolipoprotein C-II.
Apolipoprotein C-II (apoC-II) is a small protein found associated with the plasma lipoproteins. It serves a unique function in the activation of the enzyme lipoprotein lipase (triacylglycerol acyl-hydrolase, EC 3.1.1.3). ApoC-II contains a single arginine residue, permitting tryptic cleavage into two peptides after succinylation of the native protein. The succinylated amino-terminal peptide, approximately 50 residues, did not activate lipoprotein lipase. The succinylated carboxyl-terminal peptide, about 29 residues, had significant cofactor activity. Relative to native apoC-II, the maximal activation observed with the succinylated carboxyl-terminal peptide was 50% lower and the concentration required for half-maximal activity was approximately 10 times higher. Mixtures of the carboxyl- and amino-terminal peptides had no more activity than the carboxyl-terminal peptide alone. Localization of functional properties to the carboxyl region is a feature also common to apolipoproteins C-III, A-II, and A-I.
D000595:Amino Acid Sequence; D001053:Apolipoproteins / Q000378:metabolism*; D004789:Enzyme Activation; D007700:Kinetics; D008071:Lipoprotein Lipase / Q000378:metabolism*; D010455:Peptides / Q000494:pharmacology; D013329:Structure-Activity Relationship
D001053:Apolipoproteins; D010455:Peptides; D008071:Lipoprotein Lipase
J Biol Chem. 1955 Jul;215(1):15-26
14392138
Biochim Biophys Acta. 1971 Apr 27;236(1):29-37
5577467
Anal Biochem. 1967 Jul;20(1):150-4
4166536
Biochem J. 1971 Oct;124(4):677-83
4257093
Science. 1966 May 27;152(3726):1253-5
4160819
J Clin Invest. 1974 Nov;54(5):1107-24
4370795
J Biol Chem. 1974 Aug 25;249(16):5314-20
4369320
J Biol Chem. 1974 Apr 10;249(7):2220-7
4362067
Biochem Biophys Res Commun. 1972 Dec 18;49(6):1444-51
4344811
Biochemistry. 1973 Apr 24;12(9):1828-33
4349259
Biochemistry. 1973 Jan 30;12(3):502-7
4345806
Biochim Biophys Acta. 1973 Jan 19;296(1):116-23
4348316
Circ Res. 1973 Oct;33(4):403-11
4355035
J Biol Chem. 1973 Dec 25;248(24):8449-56
4357738
J Biol Chem. 1973 Jul 25;248(14):4941-6
4352183
Biochim Biophys Acta. 1970 Mar 31;200(3):573-5
4985344
J Biol Chem. 1971 May 10;246(9):2842-8
5102928
Circ Res. 1970 Oct;27(4):595-600
5507034
J Biol Chem. 1970 Dec 25;245(24):6588-94
5482770
Biochem Biophys Res Commun. 1970 Oct 9;41(1):57-62
5459123
Biochemistry. 1968 May;7(5):1941-50
5689844
Science. 1966 Aug 5;153(3736):640-1
5940417