Inhibition of erythrocyte sickling in vitro by DL-glyceraldehyde.
Concentrations of DL-glyceraldehyde between 5 and 20 mM reduce the sickling of S/S erythrocytes even in the complete absence of oxygen; at 10 mM glyceraldehyde the increase in the number of normal cells ranges from 20 to 40%. The inhibition of sickling was both concentration- and time-dependent and was not reversed by repeated washings with buffer. Incubation of erythrocytes with increasing concentrations of glyceraldehyde resulted in only a small increase in the oxygen affinity, a moderate reduction in the Hill coefficient, a substantial increase in the minimum gelling concentration, and modification of up to two lysine residues per hemoglobin molecule.
D000755:Anemia, Sickle Cell / Q000097:blood* / Q000473:pathology; D004913:Erythrocytes, Abnormal / Q000187:drug effects* / Q000473:pathology; D005782:Gels; D005985:Glyceraldehyde / Q000494:pharmacology*; D006451:Hemoglobin, Sickle / Q000378:metabolism; D006801:Humans; D008239:Lysine; D010100:Oxygen / Q000097:blood; D014633:Valine
D005782:Gels; D006451:Hemoglobin, Sickle; D005985:Glyceraldehyde; D014633:Valine; D008239:Lysine; D010100:Oxygen
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