title: Noncovalent interaction of the NH2-terminal fragment of human somatotropin with the COOH-terminal fragment of human choriomammotropin to generate growth-promoting activity.
abstract: Complementation of the plasmin fragments of reduced-carbamoylmethylated (Cam) human somatotropin (hGH) with those of reduced-carbamoylmethylated human chorionic somatomammotropin (hCS) have been investigated. It was found that the recombinant obtained by noncovalent interaction of [Cys(Cam)53]hGH-(1-134) with [Cys(Cam)-165,182,189]hCS-(141-191) exhibits 50% growth-promoting activity and nearly full immunoreactivity. Complementation of [Cys(Cam)53]hCS-(1-133) with the COOH-terminal fragment of hGH generated lower growth-promoting and immunological activities.
mesh_terms: D000595:Amino Acid Sequence; D006128:Growth / Q000187:drug effects; D013006:Growth Hormone / Q000276:immunology / Q000494:pharmacology; D006801:Humans; D010446:Peptide Fragments; D010928:Placental Lactogen / Q000276:immunology / Q000494:pharmacology; D011863:Radioimmunoassay; D013329:Structure-Activity Relationship
chemical_list: D010446:Peptide Fragments; D013006:Growth Hormone; D010928:Placental Lactogen
references.citation: Endocrinology. 1949 Nov;45(5):455-63, illust
references.pmid: 15396704
references.citation: Proc Natl Acad Sci U S A. 1976 Oct;73(10):3463-5
references.pmid: 62361
references.citation: Proc Natl Acad Sci U S A. 1977 Mar;74(3):1016-9
references.pmid: 265545
references.citation: Int J Pept Protein Res. 1977 Nov;10(5):385-93
references.pmid: 604288
references.citation: Proc Natl Acad Sci U S A. 1974 Apr;71(4):1197-201
references.pmid: 4275394
references.citation: Arch Biochem Biophys. 1974 Oct;164(2):571-4
references.pmid: 4282220
references.citation: Proc Natl Acad Sci U S A. 1976 May;73(5):1476-9
references.pmid: 58417
references.citation: Arch Biochem Biophys. 1973 Mar;155(1):95-110
references.pmid: 4712450
references.citation: Endocrinology. 1966 Aug;79(2):275-80
references.pmid: 5921766