Synthesis of chloroplast ribonucleic acid in Chlamydomonas  reinhardtii toluene-treated cells.
Chlamydomonas reinhardtii cells treated with toluene at 0 degrees C and 25 degrees C incorporate ribonucleoside triphosphates (NTPs) into chloroplast RNA at 25 degrees C and also at 35 degrees C. The incorporation requires all four NTPs and Mg2+, and is completely inhibited by DNase, RNase, actinomycin D (40 microgram/ml) and rifampicin (350 microgram/ml).  However, the incorporation is almost totally insensitive to both alpha-amanitin and streptolydigin at 200 microgram/ml.
D000546:Amanitins / Q000494:pharmacology; D000617:Aminoglycosides; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002463:Cell Membrane Permeability / Q000187:drug effects; D002696:Chlamydomonas / Q000187:drug effects / Q000378:metabolism*; D002736:Chloroplasts / Q000187:drug effects / Q000378:metabolism*; D003609:Dactinomycin / Q000494:pharmacology; D007700:Kinetics; D012313:RNA / Q000096:biosynthesis*; D012265:Ribonucleotides / Q000378:metabolism; D012293:Rifampin / Q000494:pharmacology; D014050:Toluene / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects*
D000546:Amanitins; D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D012265:Ribonucleotides; D003609:Dactinomycin; D014050:Toluene; D012313:RNA; D012293:Rifampin