title: Separation and infectivity of circular and linear forms of potato spindle tuber viroid.
abstract: Potato spindle tuber viroid can be separated into two fractions by polyacrylamide gelelectrophoresis in the presence of formamide and urea. One fraction contains predominantly circular molecules; the second fraction contains almost exclusively linear molecules. The purity of the fractions was estimated by electron microscopy of formaldehyde-denatured molecules. The length distributions of denatured circular and linear molecules were determined. Both circular and linear molecules were found to be infectious.
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D008854:Microscopy, Electron; D010941:Plant Tumors / Q000382:microbiology*; D010942:Plant Viruses / Q000502:physiology* / Q000648:ultrastructure
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