Rapid determination of serum 5-fluorocytosine levels by high-performance liquid chromatography.
A method for the rapid, quantitative determination of 5-fluorocytosine (5-FC) in serum by high-performance liquid chromatography (HPLC) has been developed. After initially ultrafiltrating the serum, a portion was injected onto a cation exchange column. 5-FC was separated by using an ammonium-phosphate buffer as the mobile phase and detected by ultraviolet absorption at 254 nm. Quantitation of 5-FC was based on the linear relationship between peak area in the chromatograms and known concentrations of 5-FC in a set of serum standards (prepared by adding from 10 to 200 micrograms of 5-FC to 1-ml aliquots of pooled human serum). This method was compared with the standard microbiological method for 5-FC. Advantages of the HPLC method include: Determination of 5-FC levels within 30 min; lack of interference from other antimicrobial drugs, particularly amphotericin B; more accurate determination of true 5-FC level, particularly at concentrations of less than 25 micrograms/ml or greater than 100 micrograms/ml; and ease with which the assay may be automated for routine use.
D001681:Biological Assay; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005437:Flucytosine / Q000097:blood*; D006801:Humans
D005437:Flucytosine
Ann Intern Med. 1977 Mar;86(3):319-21
320931
J Infect Dis. 1977 Mar;135(3):414-22
850079
Antimicrob Agents Chemother. 1975 Apr;7(4):462-5
1096801
Antimicrob Agents Chemother. 1972 Jun;1(6):476-82
4680811
J Pharm Sci. 1975 Aug;64(8):1334-9
1151707
Clin Chem. 1976 Jun;22(6):772-6
1277460
N Engl J Med. 1974 Jan 3;290(1):30-2
4593887
Biochem Pharmacol. 1966 Apr;15(4):435-46
5955812