title: Synthesis of opiate peptides by a clonal pituitary tumor cell line.
abstract: Clonal mouse pituitary tumor cells, AtT-20, synthesize at least four species of peptides with opiate activity. The endorphin concentration of AtT-20 cells was estimated to be 300-600 pmol/mg of protein. The two most abundant endorphins with apparent molecular weights of 1800 and 2400 were purified 300- and 24-fold, respectively; additional minor components were found with apparent molecular weights of greater than 3000 and less than 750.
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D001681:Biological Assay; D002460:Cell Line; D002999:Clone Cells; D004558:Electric Stimulation; D004723:Endorphins / Q000096:biosynthesis*; D004745:Enkephalins / Q000096:biosynthesis* / Q000494:pharmacology; D004789:Enzyme Activation; D006168:Guinea Pigs; D007082:Ileum / Q000187:drug effects / Q000502:physiology; D007700:Kinetics; D009270:Naloxone / Q000494:pharmacology; D009842:Oligopeptides / Q000096:biosynthesis*; D010452:Peptide Biosynthesis; D010447:Peptide Hydrolases / Q000494:pharmacology; D010911:Pituitary Neoplasms / Q000378:metabolism; D011458:Prostaglandins E / Q000494:pharmacology
chemical_list: D004723:Endorphins; D004745:Enkephalins; D009842:Oligopeptides; D011458:Prostaglandins E; D009270:Naloxone; D010447:Peptide Hydrolases; D000262:Adenylyl Cyclases
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