Minimal length of the lactose operator sequence for the specific recognition by the lactose repressor.
A number of specific duplex DNA sequences which correspond to varying lengths of the lactose operator region have been synthesized by a combination of chemical and enzymatic methods. Repressor binding studies on these synthetic duplex operator molecules show that all the nucleotides essential for full lactose operator-repressor interactions are included in a 17-nucleotide-long duplex DNA that constitutes the minimal recognition sequence for this DNA-protein interaction.
D001483:Base Sequence; D004247:DNA / Q000138:chemical synthesis; D004269:DNA, Bacterial / Q000378:metabolism*; D004794:Enzyme Repression; D005809:Genes, Regulator; D007785:Lactose / Q000378:metabolism*; D009876:Operon; D011485:Protein Binding; D013329:Structure-Activity Relationship
D004269:DNA, Bacterial; D004247:DNA; D007785:Lactose
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