title: Phage T4-modified RNA polymerase transcribes T4 late genes in vitro.
abstract: Initiation of T4 late RNA synthesis has been achieved in an in vitro system prepared from Escherichia coli cells infected with wild-type or maturation-defective mutant T4 phage. The system uses a cellophane membrane as a mechanical support for concentrated cell lysates and for added streptolydigin-resistant RNA polymerases. Transcriptional activity and selectivity of added RNA polymerases are tested while endogenous RNA polymerase activity is inhibited by streptolydigin. T4-modified RNA polymerase is required for substantial stimulation of T4 late RNA synthesis.
mesh_terms: D003090:Coliphages / Q000201:enzymology / Q000235:genetics*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D005796:Genes; D007700:Kinetics; D012367:RNA, Viral / Q000096:biosynthesis; D013698:Templates, Genetic; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D012367:RNA, Viral; D014157:Transcription Factors; D012321:DNA-Directed RNA Polymerases
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