title: Interference with thyroid histogenesis by inhibitors of collagen synthesis.
abstract: Histogenesis of thyroid follicles in the chick embryo begins with a penetration by cells of the mesenchymal capsule into a solid epithelial primordium. Before penetration occurs, slits containing fibrillar material form between the epithelial cells. The fibrillar material is an epithelial cell product as shown by its formation within channels that form in cultures of isolated epithelial primordia. The drugs L-azetidine-2-carboxylic acid (LACA) and alpha, alpha'-dipyridyl, which interfere with collagen synthesis, prevent the formation of fibrils in cultured epithelial primordia and in cultures of whole thyroids. Furthermore, mesenchymal cells do not invade when whole thyroid primordia are cultured in the presence of either drug. The effects of alpha, alpha'-dipyridyl are reversed by washing out the drug; the effects of LACA are reversed by incubation with equimolar or greater amounts of L-proline added to the medium along with the drug. The results are interpreted to mean that the fibrillar material is collagen of epithelial origin, that the collagen in some way plays a role in mesenchymal penetration of the epithelial primordium, and that the epithelium is responsible for the pattern of lobulation within the developing gland.
mesh_terms: D015082:2,2'-Dipyridyl / Q000494:pharmacology*; D000818:Animals; D001383:Azetidinecarboxylic Acid / Q000494:pharmacology*; D001385:Azetines / Q000494:pharmacology*; D002478:Cells, Cultured; D002642:Chick Embryo; D003094:Collagen / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D008854:Microscopy, Electron; D011725:Pyridines / Q000494:pharmacology*; D013961:Thyroid Gland / Q000187:drug effects / Q000196:embryology* / Q000648:ultrastructure
chemical_list: D001385:Azetines; D011725:Pyridines; D015082:2,2'-Dipyridyl; D001383:Azetidinecarboxylic Acid; D003094:Collagen
references.citation: J Cell Biol. 1965 May;25:407-8
references.pmid: 14287192
references.citation: J Morphol. 1964 Sep;115:135-51
references.pmid: 14214403
references.citation: Stain Technol. 1960 Nov;35:313-23
references.pmid: 13741297
references.citation: J Biophys Biochem Cytol. 1958 Jul 25;4(4):475-8
references.pmid: 13563554
references.citation: Dev Biol. 1972 May;28(1):123-37
references.pmid: 4114379
references.citation: Symp Soc Dev Biol. 1970;29:195-249
references.pmid: 4125734
references.citation: Dev Biol. 1972 May;28(1):183-90
references.pmid: 5041193
references.citation: J Biol Chem. 1975 Feb 25;250(4):1464-71
references.pmid: 1112811
references.citation: J Cell Biol. 1973 Apr;57(1):190-213
references.pmid: 4347977
references.citation: J Cell Biol. 1971 Sep;50(3):840-58
references.pmid: 4329158
references.citation: Biochim Biophys Acta. 1966 Dec 28;130(2):383-92
references.pmid: 4226472
references.citation: In Vitro. 1973 Nov-Dec;9(3):160-6
references.pmid: 4779003
references.citation: J Embryol Exp Morphol. 1973 Apr;29(2):439-51
references.pmid: 4717977
references.citation: J Biol Chem. 1973 Jan 25;248(2):720-9
references.pmid: 4684698
references.citation: Dev Biol. 1973 Feb;30(2):374-91
references.pmid: 4703679
references.citation: Dev Biol. 1972 May;28(1):202-18
references.pmid: 5041195
references.citation: Dev Biol. 1972 May;28(1):191-201
references.pmid: 5041194
references.citation: Biochim Biophys Acta. 1969 Feb 4;175(1):142-55
references.pmid: 4885541
references.citation: Dev Biol. 1975 Aug;45(2):291-303
references.pmid: 1193300
references.citation: Dev Biol. 1975 Sep;46(1):222-6
references.pmid: 1158025
references.citation: Dev Biol. 1971 Dec;26(4):578-605
references.pmid: 5134613
references.citation: Biochim Biophys Acta. 1971 Jun 29;236(3):517-27
references.pmid: 5559959
references.citation: Biochim Biophys Acta. 1969 Feb 4;175(1):156-64
references.pmid: 5765999