Immunization of rats with polypeptide chains from torpedo acetylcholine receptor causes an autoimmune response to receptors in rat muscle.
Four polypeptide chains were purified from acetylcholine receptor of Torpedo californica electric organ. Their apparent molecular weights were 64,000, 57,000, 49,500, and 38,000. Rats immunized with any of the four chains produced antibodies that crossreacted with rat muscle receptors in vivo. Specificities of anti-chain sera were evaluated in vitro by reaction with native receptor solubilized from electric organs and muscles of several species and by inhibition of this reaction with the purified polypeptide chains. The chains are immunologically distinct from one another. Antigenic determinants comparable to each chain of torpedo receptor are found in receptor from both rat and human muscle. At least part of each of these determinants is exposed on the extracellular surface of the muscle membrane. The most immunogenic determinants on native receptor are lost on denaturation to polypeptide chains. Its component peptides are much less immunogenic than native receptor, and induce antibodies of different specificity. Anti-receptor antibodies of many specificities can cause experimental autoimmune myasthenia gravis.
D000109:Acetylcholine / Q000276:immunology*; D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D000918:Antibody Specificity; D001323:Autoantibodies; D003429:Cross Reactions; D005399:Fishes; D009132:Muscles / Q000276:immunology*; D011489:Protein Denaturation; D051381:Rats; D011950:Receptors, Cholinergic / Q000276:immunology*; D013045:Species Specificity
D000906:Antibodies; D001323:Autoantibodies; D011950:Receptors, Cholinergic; D000109:Acetylcholine
Arch Biochem Biophys. 1977 Jun;181(2):484-9
71018
Biochemistry. 1977 Oct 4;16(20):4513-20
410440
Proc Natl Acad Sci U S A. 1977 Jul;74(7):3090-4
268657
Proc Natl Acad Sci U S A. 1977 May;74(5):2130-4
266734
Proc Natl Acad Sci U S A. 1977 Oct;74(10):4685-8
270707
J Biol Chem. 1977 Dec 10;252(23):8589-96
925013
Biochemistry. 1977 Nov 1;16(22):4900-8
911800
Biochem Soc Trans. 1977;5(2):511-4
902870
Mayo Clin Proc. 1977 May;52(5):267-80
870771
Science. 1977 Apr 29;196(4289):527-9
850793
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3334-8
4128544
J Exp Med. 1976 Sep 1;144(3):739-53
182897
J Exp Med. 1976 Sep 1;144(3):726-38
182896
Science. 1973 May 25;180(4088):871-2
4706680
Cold Spring Harb Symp Quant Biol. 1976;40:203-10
820505
Ann N Y Acad Sci. 1976;274:60-79
1066997
Ann N Y Acad Sci. 1976;274:300-18
1066990
Ann N Y Acad Sci. 1976;274:254-74
1066988
Cold Spring Harb Symp Quant Biol. 1976;40:193-202
1065525
J Exp Med. 1975 Jun 1;141(6):1365-75
1127382
Nature. 1970 Aug 15;227(5259):680-5
5432063