Urate-oxidase in liver of oxonic acid treated mice.
Following a single intraperitoneal injection of oxonic acid, urate-oxidase inhibitor with optical and chromatographic properties of the injected drug was found in the supernatants of mouse liver homogenates. Maximal inhibitory activity of 40 000 g-20 minutes supernatants against Sigma urate-oxidase was found 30 minutes after injection, and it almost disappeared after six hours. In whole homogenates, urate-oxidase activity was found 50% inhibited at 30 minutes, and it returned to normal levels between 3 and 6 hours following injection. Urate-oxidase activity of resuspended nuclei-free 40 000 g particles from these homogenates showed a significative increase of activity over controls during the first three hours after injection, and it returned to a normal level between 3 and 6 hours after injection. There was, instead, no increase of activity in 40 000 g-20 minutes particles suspensions when these were separated from homogenates prepared from untreated mice and incubated with oxonic acid.
D000818:Animals; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D051379:Mice; D010094:Oxonic Acid / Q000378:metabolism / Q000494:pharmacology*; D013053:Spectrophotometry; D014227:Triazines / Q000494:pharmacology*; D014503:Urate Oxidase / Q000378:metabolism*
D014227:Triazines; D010094:Oxonic Acid; D014503:Urate Oxidase