DNA polymerase activities in differentiating mouse neuroblastoma N-18 cells.
The activities of two DNA polymerases (DNA nucleotidyltransferases) were characterized in mouse neuroblastoma clone N-18 on the basis of their apparent molecular weights (determined by sucrose density gradient centrifugation: polymerase-alpha, 7.5-8 S; polymerase-beta, 3-4 S) and relative inhibition by sulfhydryl-blocking agents. N-Ethylmaleimide (10 mM) and iodoacetamide (1.5 mM) inhibited DNA polymerase-alpha activity completely, whereas only 35-40% inhibition was observed for DNA polymerase-beta under similar conditions. DNA polymerase-alpha activity was reduced 50-70% in N-18 cells that had been induced to differentiate by 4 micro M bromodeoxyuridine, and the low molecular weight DNA polymerase-beta activity remain unchanged. With activated calf thymus DNA as template, only DNA polymerase-alpha activity was stimulated in the presence of added ribonucleotides and purified Escherichia coli RNA polymerase.
D001973:Bromodeoxyuridine / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D004256:DNA Polymerase I / Q000037:antagonists & inhibitors / Q000378:metabolism*; D004257:DNA Polymerase II / Q000037:antagonists & inhibitors / Q000378:metabolism*; D004273:DNA, Neoplasm / Q000096:biosynthesis; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D009374:Neoplasms, Experimental; D009447:Neuroblastoma / Q000201:enzymology*; D013439:Sulfhydryl Reagents / Q000494:pharmacology
D004273:DNA, Neoplasm; D013439:Sulfhydryl Reagents; D012321:DNA-Directed RNA Polymerases; D004256:DNA Polymerase I; D004257:DNA Polymerase II; D004259:DNA-Directed DNA Polymerase; D001973:Bromodeoxyuridine
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