title: Evidence from UV transcription mapping in HeLa cells that heterogeneous nuclear RNA is the messenger RNA precursor.
abstract: The effects of UV irradiation on the incorporation of [3H]uridine in HeLa (human) cell mRNA, rRNA, heterogeneous nuclear RNA (hnRNA) and early mRNA from adenovirus type 2 have been compared. The UV target size of cell mRNA is at least 3 times larger than the average size of the mRNA itself and larger than the adenovirus-2 early mRNA, which is known to derive from transcription units of about 1.5-5.0 kilobases. The UV target size of hnRNA, in contrast, is about the same as its size determined by sedimentation and overlaps with the target size of mRNA. It is concluded that most mRNA derives from a higher molecular weight hnRNA molecule.
mesh_terms: D000256:Adenoviridae / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism*; D006367:HeLa Cells / Q000528:radiation effects; D006801:Humans; D008970:Molecular Weight; D012313:RNA / Q000502:physiology* / Q000528:radiation effects; D012333:RNA, Messenger / Q000096:biosynthesis*; D012367:RNA, Viral / Q000378:metabolism; D014158:Transcription, Genetic / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D012333:RNA, Messenger; D012367:RNA, Viral; D012313:RNA
references.citation: Science. 1959 Aug 21;130(3373):432-7
references.pmid: 13675766
references.citation: Cell. 1977 Jul;11(3):641-50
references.pmid: 884738
references.citation: J Mol Biol. 1976 Sep 15;106(2):403-20
references.pmid: 978727
references.citation: Cell. 1976 Dec;9(4 PT 2):775-83
references.pmid: 1017014
references.citation: Cell. 1977 Apr;10(4):617-21
references.pmid: 862023
references.citation: Cell. 1977 Apr;10(4):611-6
references.pmid: 862022
references.citation: Cell. 1977 Feb;10(2):153-66
references.pmid: 837446
references.citation: Cell. 1976 Nov;9(3):465-72
references.pmid: 991275
references.citation: Cold Spring Harb Symp Quant Biol. 1975;39 Pt 1:447-56
references.pmid: 1057473
references.citation: J Virol. 1973 Oct;12(4):882-6
references.pmid: 4591054
references.citation: J Mol Biol. 1973 Apr 15;75(3):515-32
references.pmid: 4125135
references.citation: J Mol Biol. 1975 Jan 25;91(3):235-56
references.pmid: 1237628
references.citation: Nature. 1974 Oct 18;251(5476):639-41
references.pmid: 4608761
references.citation: Proc Natl Acad Sci U S A. 1973 Oct;70(10):2827-31
references.pmid: 4517935
references.citation: Proc Natl Acad Sci U S A. 1976 Feb;73(2):442-6
references.pmid: 174107
references.citation: Cell. 1976 Feb;7(2):227-37
references.pmid: 954080
references.citation: Proc Natl Acad Sci U S A. 1975 Nov;72(11):4445-9
references.pmid: 1060124
references.citation: Biochim Biophys Acta. 1970;209(2):368-86
references.pmid: 5453524
references.citation: J Mol Biol. 1966 May;17(1):117-30
references.pmid: 5961144