Interacting cell populations in cultures of leukocytes from normal or leukemic peripheral blood.
Kinetic studies in cultures containing 2 X 10(5) peripheral leukocytes from patients with acute myelobastic leukemia revealed extensive, radiation-sensitive increases in thymidine incorporation without parallel increases in cell number. Modest and variable stimulation of 3HTdR incorporation was seen with the addition of either leukocyte-conditioned medium prepared with phytohemagglutin (PHA) or PHA alone. However, using the method of limiting dilution, stimulation was always observed and ranged from 3- to 20-fold in individual patients. By mixing small numbers of intact cells with larger numbers of irradiated autologous cells, quantitative evidence was obtained for a cellular interaction between irradiated, PHA-stimulated populations capable of 3HTdR incorporation. Similar evidence for cell-cell interaction was obtained for normal leukocytes.
D002454:Cell Differentiation; D002471:Cell Transformation, Neoplastic / Q000528:radiation effects; D002478:Cells, Cultured; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D007700:Kinetics; D037102:Lectins / Q000494:pharmacology; D015470:Leukemia, Myeloid, Acute / Q000097:blood*; D007958:Leukocyte Count; D007962:Leukocytes / Q000166:cytology* / Q000528:radiation effects; D013936:Thymidine / Q000378:metabolism
D037102:Lectins; D013936:Thymidine