title: Anti-epidermal-cell-surface pemphigus antibody detaches viable epidermal cells from culture plates by activation of proteinase.
abstract: Immunoglobulin from pemphigus patients binds to the surface of mouse epidermal cells in culture. Cells incubated with the pemphigus antibody are easily detached from culture plates whereas cells incubated with serum from normal patients remain on the plate. Pemphigus antibody-mediated cell detachment is blocked by the addition of the proteinase inhibitors soybean trypsin inhibitor and alpha2-macroglobulin to the culture media. Detachable cells are viable, and activation of the complement cascade is not necessary for cell detachment. The anti-cell-surface antibody of pemphigus appears to disrupt adhesion between viable epidermal cells by activation of proteinase.
mesh_terms: D000906:Antibodies; D000937:Antigen-Antibody Reactions; D002448:Cell Adhesion / Q000187:drug effects; D002470:Cell Survival; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D004789:Enzyme Activation; D004817:Epidermis / Q000201:enzymology / Q000276:immunology*; D006801:Humans; D007140:Immunoglobulin Fab Fragments; D010392:Pemphigus / Q000276:immunology*; D011480:Protease Inhibitors
chemical_list: D000906:Antibodies; D007140:Immunoglobulin Fab Fragments; D011480:Protease Inhibitors; D003208:Concanavalin A
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