Sequence relationship between long and short repetitive DNA of the rat: a preliminary report.
Long and short repetitive sequences of rat DNA can be isolated and characterized. Long [greater than 1.5 kilobases (kb)] sequences can be separated from short (0.2-0.4 kb) sequences by exclusion chromatography after renaturation of 4-kb DNA fragments to a repetitive Cot and digestion with the single-strand-specific S1 nuclease. (Cot is the initial concentration of DNA in mol of nucleotides/liter multiplied by time in sec.) Long repetitive DNA can be driven by an excess of whole rat DNA can also be used to drive tracer quantities of either long (self-renaturation) or short repetitive DNA. Both the extent and the rate of the renaturations are found to be similar, suggesting that long and short DNA fragments share sequences. When long repetitive DNA is used to drive whole DNA tracers of various lengths, a 3.2-kb interspersion period is found. These data are consistent with the concept that short repetitive sequences are present within long repetitive DNA sequences in the rat genome.
D000818:Animals; D001483:Base Sequence; D004247:DNA; D009695:Nucleic Acid Renaturation; D051381:Rats
D004247:DNA
J Mol Biol. 1965 Feb;11:373-90
14290352
Nucleic Acids Res. 1977;4(5):1553-67
896469
Nucleic Acids Res. 1977 Jun;4(6):1727-37
896473
Cell. 1975 Nov;6(3):345-58
1052772
J Mol Evol. 1976 Dec 31;9(1):1-23
1018329
Nature. 1967 Jul 22;215(5099):360-3
6058287
Q Rev Biol. 1973 Dec;48(4):565-613
4592421
Chromosoma. 1975 Jul 21;51(3):225-51
238802
Cold Spring Harb Symp Quant Biol. 1974;38:501-5
4524770
Cold Spring Harb Symp Quant Biol. 1974;38:295-301
4524760
Proc Natl Acad Sci U S A. 1974 Jul;71(7):2900-4
4527857
J Mol Biol. 1973 Jun 15;77(1):1-23
4769838
Proc Natl Acad Sci U S A. 1976 Jul;73(7):2289-93
1065878
Cell. 1975 Sep;6(1):29-39
1164733
Science. 1969 Jul 25;165(3891):349-57
5789433