title: Genetic dissection of the control of normal differentiation in myeloid leukemic cells.
abstract: Normal myeloid precursors and MGI(+)D(+) myeloid leukemic cells can be induced to differentiate to mature cells by the normal protein inducer MGI. The sequence of differentiation is the induction of C3 and Fc rosettes, C3 and Fc immune phagocytosis (IP), synthesis and secretion of lysozyme, and formation of mature macrophages and granulocytes. Mutant clones of myeloid leukemic cells have been isolated with differences in the time of induction of C3 and Fc rosettes and C3 and Fc IP, in which lysozyme was induced without going through the stage of Fc or C3 IP, and with differences in inducibility by MGI to mature macrophages or granulocytes. Only one out of five MGI(-)D(-) clones gave rise to MGI(+)D(+) mutants. The ability to obtain mutants from this clone was associated with its special chromosome constitution, and these mutants showed a change in their ability for cap formation by concanavalin A. The steroid inducer dexamethasone can induce in MGI(+)D(+) clones differentiation to macrophages but not to granulocytes. Differentiation by steroid inducer in different clones occurred either with or without induction of Fc rosettes and Fc IP, and induction of C3 rosettes was not always associated with induction of C3 IP. The use of mutants that differ in their competence to be induced by MGI or steroid inducer has shown that there are separate controls for the induction of C3 and Fc rosettes, C3 and Fc IP, lysozyme, macrophages, and granulocytes.
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D003176:Complement C3 / Q000378:metabolism; D006098:Granulocytes / Q000166:cytology; D007141:Immunoglobulin Fc Fragments / Q000378:metabolism; D007152:Immunologic Capping; D007942:Leukemia, Experimental / Q000235:genetics / Q000473:pathology; D007951:Leukemia, Myeloid / Q000235:genetics / Q000473:pathology*; D008264:Macrophages / Q000166:cytology; D009113:Muramidase / Q000378:metabolism; D010587:Phagocytosis; D012397:Rosette Formation
chemical_list: D003176:Complement C3; D007141:Immunoglobulin Fc Fragments; D009113:Muramidase
references.citation: J Natl Cancer Inst. 1963 Jul;31:1-39
references.pmid: 14043037
references.citation: Proc Natl Acad Sci U S A. 1977 Mar;74(3):1100-4
references.pmid: 265555
references.citation: J Cell Physiol. 1977 Jul;92(1):97-108
references.pmid: 197112
references.citation: Proc Natl Acad Sci U S A. 1977 Jan;74(1):253-7
references.pmid: 264678
references.citation: Cell. 1976 Dec;9(4 PT 2):675-84
references.pmid: 1017012
references.citation: J Cell Physiol. 1976 Oct;89(2):259-66
references.pmid: 972167
references.citation: Exp Cell Res. 1974 Oct;88(2):257-62
references.pmid: 4529737
references.citation: Aust J Exp Biol Med Sci. 1966 Jun;44(3):287-99
references.pmid: 4164182
references.citation: J Cell Physiol. 1969 Dec;74(3):223-34
references.pmid: 4187945
references.citation: J Cell Physiol. 1971 Apr;77(2):121-34
references.pmid: 5572424
references.citation: J Exp Med. 1970 Oct 1;132(4):702-20
references.pmid: 4101362
references.citation: Proc Natl Acad Sci U S A. 1976 Apr;73(4):1232-6
references.pmid: 177980
references.citation: Harvey Lect. 1974;68:1-35
references.pmid: 4375672
references.citation: Annu Rev Biochem. 1974;43(0):169-214
references.pmid: 4368906
references.citation: Proc Natl Acad Sci U S A. 1971 Oct;68(10):2540-4
references.pmid: 4332814
references.citation: Proc Natl Acad Sci U S A. 1973 May;70(5):1442-6
references.pmid: 4514314
references.citation: Proc Natl Acad Sci U S A. 1973 Feb;70(2):343-6
references.pmid: 4510279
references.citation: Proc Natl Acad Sci U S A. 1974 Sep;71(9):3507-11
references.pmid: 4530318
references.citation: J Cell Physiol. 1975 Jun;85(3):587-94
references.pmid: 124742
references.citation: J Exp Med. 1974 May 1;139(5):1228-48
references.pmid: 4825244
references.citation: J Exp Med. 1972 Apr 1;135(4):780-92
references.pmid: 5018051
references.citation: Proc Natl Acad Sci U S A. 1971 Feb;68(2):378-82
references.pmid: 5277089
references.citation: Int J Cancer. 1975 May 15;15(5):731-40
references.pmid: 1079793
references.citation: J Immunol. 1976 Aug;117(2):580-6
references.pmid: 950462
references.citation: Proc Natl Acad Sci U S A. 1976 Jan;73(1):188-92
references.pmid: 1061115
references.citation: J Immunol. 1976 Apr;116(4):892-7
references.pmid: 1254970
references.citation: J Cell Physiol. 1975 Oct;86(2 Pt 1):221-30
references.pmid: 1194362
references.citation: Proc Natl Acad Sci U S A. 1970 Nov;67(3):1542-9
references.pmid: 5274478
references.citation: J Exp Med. 1975 Jun 1;141(6):1278-90
references.pmid: 1127381
references.citation: Proc Natl Acad Sci U S A. 1966 Aug;56(2):488-95
references.pmid: 5229970
references.citation: J Cell Physiol. 1969 Dec;74(3):323-32
references.pmid: 5390860
references.citation: Exp Cell Res. 1966 Oct;43(3):553-63
references.pmid: 5957746
references.citation: J Cell Physiol. 1965 Dec;66(3):319-24
references.pmid: 5884359