Loss of infectivity of brome mosaic virus RNA after chemical modification of the 3' or 5' terminus.
Brome mosaic virus (BMV) RNA that had both termini chemically modified by periodate oxidation and aniline-catalyzed cleavage of the terminal nucleotide had drastically reduced infectivity. BMV RNA that was first enzymatically tyrosylated to protect the 3' terminus from modification, and then modified at the 5' terminus by periodate oxidation and aniline cleavage, had a similar reduction in infectivity. Tyrosylation followed by acetylation modifies only the 3' terminus. Nevertheless, acetylated tyrosyl-BMV RNA was less than one-fourth as infectious as a control sample subjected to procedures that differed only by the presence of tyrosinol (which prevents aminoacylation and subsequent acetylation). For each modified form of viral RNA, care was taken to test the infectivity of appropriate control samples. The integrity of the modified RNAs was examined by gel electrophoresis and by biological translation and aminoacylation assays. We conclude that, in different ways, both the 5'- and 3'-terminal structures of BMV RNA play important roles during infection of the host.
D001483:Base Sequence; D009029:Mosaic Viruses / Q000254:growth & development*; D010942:Plant Viruses / Q000254:growth & development*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism; D013329:Structure-Activity Relationship; D013698:Templates, Genetic; D014443:Tyrosine / Q000378:metabolism; D014779:Virus Replication
D012333:RNA, Messenger; D012367:RNA, Viral; D014443:Tyrosine
J Virol. 1976 Oct;20(1):117-22
978788
J Gen Virol. 1974 Nov;25(2):257-61
4373529
Biochem J. 1972 Oct;129(4):969-76
4655830
Biochemistry. 1967 Mar;6(3):827-36
4290596
Biochemistry. 1966 Aug;5(8):2735-43
4290988
Proc Natl Acad Sci U S A. 1975 Jan;72(1):362-6
1054511
Nature. 1975 May 1;255(5503):33-7
165427
Proc Natl Acad Sci U S A. 1975 Mar;72(3):1189-93
165487
Proc Natl Acad Sci U S A. 1972 May;69(5):1313-7
4624757
J Virol. 1973 Dec;12(6):1434-41
4586778
Ann Microbiol (Paris). 1976 Jan;127A(1):143-52
179456
Proc Natl Acad Sci U S A. 1975 Jan;72(1):318-22
164018
Nucleic Acids Res. 1975 Jul;2(7):1189-201
168558
FEBS Lett. 1975 Sep 1;57(1):31-4
170129
Biochem Biophys Res Commun. 1975 Oct 27;66(4):1186-93
172074
FEBS Lett. 1975 Jan 1;49(3):385-9
162886
Proc Natl Acad Sci U S A. 1976 Feb;73(2):327-30
174102
J Mol Biol. 1975 Dec 25;99(4):519-47
175165
J Virol. 1976 Apr;18(1):260-7
176464
Nature. 1974 May 24;249(455):353-5
4841365
J Virol. 1976 Aug;19(2):637-42
957483
Proc Natl Acad Sci U S A. 1975 Feb;72(2):742-5
1054852
Proc Natl Acad Sci U S A. 1976 Jan;73(1):19-23
1061116
Biochim Biophys Acta. 1967 Sep 26;145(2):292-9
6064630
J Mol Biol. 1965 Aug;13(1):127-37
5859032