[Kinetics of specific (codon-dependent) binding of aminoacyl-tRNA to the 30S ribosomal subunit under different medium conditions].
It was shown that the increase of Mg2+ ions concentration in the medium from 0,01 M to 0,03 M speeds up the formation of a codon-dependent complex between 14C-Phe-tRNA and the 30S ribosomal subparticle. Under high MgCl2 concentration (0,02 M) the increase of NH4Cl concentration also accelerates the specific binding of 14C-Phe-tRNA to the 30S subparticle. In the presence of 0,005 M MgCl2 0,5 M urea significantly decreases the rate of the specific binding. 0,5 M ethanol does not have any noticeable effect on the kinetics of the reaction.
D000643:Ammonium Chloride / Q000494:pharmacology; D003062:Codon; D007700:Kinetics; D008274:Magnesium / Q000008:administration & dosage / Q000494:pharmacology; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism*; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism*; D014508:Urea / Q000494:pharmacology
D003062:Codon; D012346:RNA, Transfer, Amino Acyl; D000643:Ammonium Chloride; D014508:Urea; D008274:Magnesium
Izuchenie kinetiki spetsificheskogo (kodonzavisimogo) sviazyvaniia aminoatsil-tRNK s 30 S-ribosomnoĭ subchastitseĭ v razlichnykh usloviiakh sredy.