Inhibition of deacylation and improvement in N-hydroxysuccinimide ester modification of phenylalanyl-tRNA by dimethyl sulfoxide.
We have found that dimethyl sulfoxide significantly inhibits deacylation of Phe-tRNA. This allows a high pH in N-hydroxysuccinimide ester reactions while maintaining a high level of aminoacylated tRNA, improving the overall yield of the Phe-tRNA modification reaction.
D055598:Chemical Phenomena; D002621:Chemistry; D004121:Dimethyl Sulfoxide; D007700:Kinetics; D010649:Phenylalanine; D012346:RNA, Transfer, Amino Acyl; D013388:Succinimides
D012346:RNA, Transfer, Amino Acyl; D013388:Succinimides; D010649:Phenylalanine; C001426:N-hydroxysuccinimide; D004121:Dimethyl Sulfoxide