title: Vibrio cholerae hybrid sex factor that contains ampicillin transposon Tn1.
abstract: The ampicillin resistance transposon Tn1 was translocated from the R plasmid RP4 to the Vibrio cholerae conjugative plasmid, P. The hybrid sex factor P::Tn1 was highly transmissible and expressed the biological activities of the P factor. In addition, P::Tn1 facilitated transfer of RP4 to V. cholerae recipients. Physical studies of P::Tn1 indicated that the Tn1 transposon was added to the otherwise unaltered P plasmid.
mesh_terms: D000667:Ampicillin / Q000494:pharmacology*; D002499:Centrifugation, Density Gradient; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000235:genetics*; D005144:F Factor; D005796:Genes; D008854:Microscopy, Electron; D010403:Penicillin Resistance; D010957:Plasmids; D014734:Vibrio cholerae / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D000667:Ampicillin
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