Chromatin structure in the cellular slime mold Dictyostelium discoideum.
The structure of Dictyostelium discoideum chromatin has been studied by the following techniques: electron microscopy, staphylococcal nuclease digestion, acrylamide gel electrophoresis, sucrose gradient centrifugation, and melting. The basic unit of chromatin is the nucleosome, which is a particle 98.6 A in diameter. Approximately 50% of the chromatin is protected from nuclease digestion, but this decreases when protease activity is not inhibited. The nucleosome contains 187 base pairs of DNA, including a 137-base-pair core and a 50-base-pair linker. The monomer nucleosome has an s20,w value of 11.5 S on isokinetic sucrose gradients. When the chromatin is melted, four transitions are observed, at 54.5 degrees, 66.7 degress, 74.9 degrees, and 79.7 degrees. The structure of Dictyostelium chromatin is very similar to that seen in higher eukaryotes.
D002499:Centrifugation, Density Gradient; D002843:Chromatin / Q000648:ultrastructure*; D004247:DNA / Q000032:analysis; D003851:Deoxyribonucleases; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000032:analysis; D008854:Microscopy, Electron; D009235:Myxomycetes; D014357:Trypsin
D002843:Chromatin; D006657:Histones; D004247:DNA; D003851:Deoxyribonucleases; D014357:Trypsin
Can J Biochem Physiol. 1959 Dec;37:1393-9
14405350
Nature. 1953 Apr 25;171(4356):737-8
13054692
Proc Natl Acad Sci U S A. 1977 May;74(5):2157-61
17120
Cell. 1976 Dec;9(4 Pt 1):605-13
1034500
Cell. 1976 Dec;9(4 Pt 1):627-32
1009580
FEBS Lett. 1976 Oct 15;69(1):281-5
992040
Biochem Biophys Res Commun. 1977 Jan 10;74(1):230-4
402131
Annu Rev Biochem. 1977;46:931-54
332067
Proc Natl Acad Sci U S A. 1977 Aug;74(8):3244-8
269387
Proc Natl Acad Sci U S A. 1977 May;74(5):1879-83
266711
Nature. 1977 Sep 1;269(5623):29-36
895884
J Mol Biol. 1977 Jan 25;109(3):393-404
833849
Proc Natl Acad Sci U S A. 1976 Dec;73(12):4382-6
826906
Nature. 1976 Dec 9;264(5586):517-22
1004586
Nucleic Acids Res. 1976 Dec;3(12):3271-91
1005117
J Mol Biol. 1972 May 28;66(3):363-77
5064459
J Cell Biol. 1970 May;45(2):399-407
5535143
Nature. 1967 Jul 22;215(5099):360-3
6058287
Science. 1974 Jan 25;183(4122):330-2
4128918
Biochemistry. 1969 Aug;8(8):3214-8
5809221
Acta Endocrinol Suppl (Copenh). 1972;168:155-77
4562652
Cell. 1976 Jul;8(3):349-55
133762
Biochemistry. 1974 May 7;13(10):2098-104
4857060
Science. 1974 May 24;184(4139):865-8
4825888
J Mol Biol. 1970 Mar;48(3):469-87
4911812
Biochemistry. 1968 Feb;7(2):668-74
4868544
Cell. 1976 Jul;8(3):357-63
782724
Proc Natl Acad Sci U S A. 1976 Feb;73(2):563-7
1108025
Cell. 1975 Sep;6(1):85-110
1164735
Nature. 1975 Jan 24;253(5489):245-9
1167623
FEBS Lett. 1975 Feb 1;50(2):195-9
1112411
Cell. 1975 Apr;4(4):281-300
1122558
Biochemistry. 1971 Apr 13;10(8):1461-70
5580664
J Biol Chem. 1968 Sep 10;243(17):4500-5
5684006