Comparison of metoprine (DDMP) and etoprine (DDEP) by measuring the inhibition of deoxyuridine incorporation into the DNA of human leukemic cells 1,2,3.
In vitro studies were used to measure the incorporation of labeled deoxyuridine into the DNA of human leukemic cells in autologous plasma in order to compare the inhibitory effects of two diaminopyrimidines, metoprine and etoprine. The results indicate that these two lipid-soluble folate antagonists give similar qualitative and quantitative effects over a concentration range of from 1 to 100 micrometer. However, drug-induced inhibition of deoxyuridine incorporation into DNA was quite variable for cells obtained from different adult patients, even within a given morphologic type of leukemia. Despite these biologic varitions in leukemic sensitivity in vitro, both drugs gave virtually identical results on an equimolar basis.
D000970:Antineoplastic Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D004273:DNA, Neoplasm / Q000096:biosynthesis*; D003857:Deoxyuridine / Q000378:metabolism*; D003864:Depression, Chemical; D006801:Humans; D007938:Leukemia / Q000378:metabolism*; D007951:Leukemia, Myeloid / Q000378:metabolism; D015470:Leukemia, Myeloid, Acute / Q000378:metabolism; D007962:Leukocytes / Q000378:metabolism; D011739:Pyrimethamine / Q000031:analogs & derivatives* / Q000494:pharmacology; D013997:Time Factors
D000970:Antineoplastic Agents; D004273:DNA, Neoplasm; C006308:metoprine; C013909:etoprine; D003857:Deoxyuridine; D011739:Pyrimethamine