title: Biosynthesis in vitro of fucose-containing glycosphingolipids in human neuroblastoma IMR-32 cells.
abstract: Two different glycolipid:fucosyltransferase activities involved in the biosynthesis in vitro of blood group-related glycosphingolipids have been detected in a membrane preparation isolated from a human neuroblastoma-derived clonal cell line, IMR-32. The membrane preparation contains an alpha (1,2)-fucosyltransferase (EC 2.4.1.89) that catalyzed the transfer of vucose from GDP--[14C]fucose to neolactotetraosylceramide or neolactopentaosylceramide to form types H-I and B-I glycolipids, respectively. The second fucosyltransferase catalyzes the transfer of fucose to lactotriaosylceramide [GlcNAc(beta1-3)Gal(beta1-4)Glc-Cer] to form a tetraglycosylceramide intermediate of the novel Lea-type glycolipid. UDP-galactose:lactotriaosylceramide beta-galactosyltransferase (EC 2.4.1.86) had 4 times the activity of UDP-galactose:alpha-galactosyltransferase (EC 2.4.1.87) when tested under similar conditions. alpha-Fucosyltransferase activities and the incorporation of [14C]fucose into glycoproteins and glycolipids were also compared in cells differentiated in the presence of 4 micron BrdUrd and 6-mercaptoguanosine.
mesh_terms: D000017:ABO Blood-Group System; D001973:Bromodeoxyuridine / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D005643:Fucose / Q000378:metabolism*; D005700:Galactosyltransferases / Q000378:metabolism; D006028:Glycosphingolipids / Q000096:biosynthesis*; D006602:Hexosyltransferases / Q000378:metabolism*; D006801:Humans; D007983:Lewis Blood Group Antigens; D009447:Neuroblastoma
chemical_list: D000017:ABO Blood-Group System; D006028:Glycosphingolipids; D007983:Lewis Blood Group Antigens; D005643:Fucose; D005700:Galactosyltransferases; D006602:Hexosyltransferases; D001973:Bromodeoxyuridine
references.citation: Jpn J Exp Med. 1963 Dec;33:347-60
references.pmid: 14148093
references.citation: Biochim Biophys Acta. 1963 Oct 29;78:313-28
references.pmid: 14099641
references.citation: Jpn J Exp Med. 1953 Aug;23(4):327-31
references.pmid: 13162652
references.citation: J Biol Chem. 1951 Nov;193(1):265-75
references.pmid: 14907713
references.citation: Biochem Biophys Res Commun. 1977 Feb 7;74(3):1001-6
references.pmid: 66052
references.citation: Biochim Biophys Acta. 1977 Mar 25;486(3):531-40
references.pmid: 192301
references.citation: Biochemistry. 1977 Mar 22;16(6):1223-8
references.pmid: 402937
references.citation: Eur J Biochem. 1976 Dec;71(1):9-18
references.pmid: 827447
references.citation: Biochem Biophys Res Commun. 1976 May 23;76(2):488-94
references.pmid: 1027442
references.citation: Nat New Biol. 1971 Sep 29;233(39):141-2
references.pmid: 4107316
references.citation: Cancer Res. 1970 Aug;30(8):2110-8
references.pmid: 5459762
references.citation: J Biochem. 1973 Feb;73(2):387-96
references.pmid: 4709622
references.citation: Arch Biochem Biophys. 1971 Dec;147(2):753-61
references.pmid: 4109333
references.citation: Hoppe Seylers Z Physiol Chem. 1973 Sep;354(9):1049-56
references.pmid: 4372157
references.citation: J Biol Chem. 1972 Mar 10;247(5):1489-95
references.pmid: 4335001
references.citation: J Biol Chem. 1971 Aug 25;246(16):5154-61
references.pmid: 4328244
references.citation: J Biol Chem. 1973 Mar 10;248(5):1700-6
references.pmid: 4632915
references.citation: Eur J Biochem. 1976 May 1;64(2):499-506
references.pmid: 6276
references.citation: Biochem Biophys Res Commun. 1976 Jul 26;71(2):472-6
references.pmid: 183771
references.citation: Biochem Biophys Res Commun. 1976 Feb 9;68(3):799-806
references.pmid: 177011
references.citation: Eur J Biochem. 1972 Oct;30(2):269-77
references.pmid: 4663380
references.citation: Chem Phys Lipids. 1975 Nov;15(2):105-24
references.pmid: 812611
references.citation: Biochem Biophys Res Commun. 1976 Jul 12;71(1):385-92
references.pmid: 822849
references.citation: J Biol Chem. 1975 Apr 25;250(8):2956-62
references.pmid: 804484
references.citation: FEBS Lett. 1976 Feb 15;62(2):161-4
references.pmid: 1253979
references.citation: J Biol Chem. 1976 Apr 25;251(8):2385-7
references.pmid: 1262328
references.citation: J Biochem. 1975 Nov;78(5):947-54
references.pmid: 1213997
references.citation: Biochem Biophys Res Commun. 1975 Oct 27;66(4):1380-8
references.pmid: 1191299
references.citation: Proc Natl Acad Sci U S A. 1970 May;66(1):160-7
references.pmid: 5273894
references.citation: Cancer Res. 1975 Apr;35(4):1035-44
references.pmid: 1116142
references.citation: Proc Natl Acad Sci U S A. 1970 Sep;67(1):247-54
references.pmid: 5272316
references.citation: J Biol Chem. 1971 Mar 10;246(5):1192-200
references.pmid: 5545063
references.citation: J Biochem. 1971 Jul;70(1):75-8
references.pmid: 5562348
references.citation: Biochemistry. 1968 Apr;7(4):1279-86
references.pmid: 5677817
references.citation: J Biol Chem. 1965 Oct;240(10):4115-7
references.pmid: 5842076