Translation in vitro of rat liver messenger RNA coding for ligandin (glutathione S-transferase B).
Poly(A)-containing rat liver mRNA isolated from animals injected with phenobarbital and uninjected controls was translated efficiently in a wheat-germ system. The synthesis of ligandin (glutathione S-transferase B; glutathione transferase; RX-gluathione R-transferase, EC 2.5.1.18) was detected by immunoprecipitation with a highly purified monospecific ligandin antibody and analysis by sodium dodecyl sulfate/polyacrylamide gel electrophoresis. The extent of incorporation of [35S]methionine into ligandin in the translation system was similar for poly(A)-containing messages from un-infected animals and those treated with phenobarbital.
D000818:Animals; D002474:Cell-Free System; D005982:Glutathione Transferase / Q000096:biosynthesis*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008970:Molecular Weight; D010634:Phenobarbital / Q000494:pharmacology; D011061:Poly A / Q000302:isolation & purification; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism*; D051381:Rats; D014908:Triticum
D012333:RNA, Messenger; D011061:Poly A; D005982:Glutathione Transferase; D010634:Phenobarbital
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