Embryonic chicken brain and mouse neuroblastoma cells N1E-115 and N-18 contain an inhibitor of acid deoxyribonuclease.
Activity of a trypsin-sensitive acid deoxyribonuclease (DNase) inhibitor has been detected in 13- to 21-day-old embryonic chicken brains and in clonal lines of neuroblastoma cells (adrenergic, N1E-115, and neurotransmitter-inactive, N-18) grown for 48 hr after subculture. The activities of purified porcine and bovine spleen DNases were inhibited 60--75% in the presence of the inhibitor, whereas less than 10% inhibition was observed with purified pancreatic DNase. Activities of an acidic DNase and its inhibitor reached maxima in 21-day-old embryonic chicken brain. The proteins were separated by isoelectric focusing and affinity column chromatographic techniques. The pI values of the acid DNase and its inhibitor were 5.4 and 4.2, respectively.
D000818:Animals; D001921:Brain / Q000196:embryology* / Q000201:enzymology; D002460:Cell Line; D002642:Chick Embryo; D003851:Deoxyribonucleases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D007526:Isoelectric Point; D051379:Mice; D009374:Neoplasms, Experimental / Q000201:enzymology; D009419:Nerve Tissue Proteins / Q000302:isolation & purification / Q000378:metabolism; D009447:Neuroblastoma / Q000201:enzymology*; D014357:Trypsin
D009419:Nerve Tissue Proteins; D003851:Deoxyribonucleases; D014357:Trypsin
Biochem J. 1956 Feb;62(2):315-23
13293190
J Gen Physiol. 1952 Nov;36(2):227-41
13011279
J Biol Chem. 1951 Nov;193(1):265-75
14907713
Nat New Biol. 1971 Sep 29;233(39):141-2
4107316
Proc Natl Acad Sci U S A. 1971 Jan;68(1):234-9
5276295
Proc Natl Acad Sci U S A. 1974 Dec;71(12):4742-6
4140510
Proc Natl Acad Sci U S A. 1972 Jan;69(1):258-63
4400294
Proc Natl Acad Sci U S A. 1972 Aug;69(8):2241-5
4403308
Biochem Biophys Res Commun. 1974 Nov 27;61(2):751-8
4375999
Science. 1974 Mar 1;183(4127):817-24
4359338
Biochemistry. 1974 Feb 26;13(5):1014-21
4360349
Biochem Biophys Res Commun. 1973 Feb 20;50(4):973-7
4347896
Nature. 1971 Oct 8;233(5319):413-5
4330071
Proc Soc Exp Biol Med. 1973 Sep;143(4):1168-71
4355242
J Biol Chem. 1973 Aug 25;248(16):5873-9
4353280
J Biol Chem. 1975 Jul 10;250(13):5003-8
1171095
Eur J Biochem. 1975 Jun 16;55(1):9-14
170099
Exp Cell Res. 1976 Jun;100(1):41-6
179831
J Biol Chem. 1976 Jan 10;251(1):116-23
396
Biochem Biophys Res Commun. 1975 Sep 2;66(1):131-8
169841
J Biol Chem. 1976 Mar 10;251(5):1471-5
176152
J Neurochem. 1968 Jun;15(6):477-81
4970072
Proc Natl Acad Sci U S A. 1975 Aug;72(8):3119-23
1059097
J Cell Physiol. 1975 Aug;86(1):71-82
1176542
J Neurochem. 1975 Dec;25(6):861-5
1206403
Cancer Res. 1975 Apr;35(4):1035-44
1116142
Proc Natl Acad Sci U S A. 1970 Sep;67(1):247-54
5272316
Nature. 1970 Aug 15;227(5259):680-5
5432063
J Biol Chem. 1969 Aug 25;244(16):4406-12
5806584