title: Translation of melittin messenger RNA in vitro yields a product terminating with glutaminylglycine rather than with glutaminamide.
abstract: Melittin messenger RNA from queen bee venom glands has been translated in a cell-free system from wheat germ. A product larger than promelittin is formed which has the carboxy-terminal sequence-Gln-Gln-GlyCOOH. Melittin and promelittin from venom glands terminate in -Gln-GlnCONH2. The possible role of the extra glycine residue in the formation of a COOH-terminal amide via a transamidase-like reaction is discussed.
mesh_terms: D000595:Amino Acid Sequence; D002474:Cell-Free System; D005973:Glutamine / Q000378:metabolism; D005998:Glycine / Q000378:metabolism; D008555:Melitten / Q000096:biosynthesis*; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000378:metabolism; D014688:Venoms / Q000096:biosynthesis*
chemical_list: D011498:Protein Precursors; D012333:RNA, Messenger; D014688:Venoms; D005973:Glutamine; D008555:Melitten; D005998:Glycine
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