Histotoxic properties of carbon monoxide in the living organ I. Fluorometry of oxidation-reduction state of the rabbit kidney in situ.
Effect of carbon monoxide on the redox state and blood flow in the renal cortex of the rabbit was examined by the fluorometric method. By a short-period (1-3 min) inhalation of CO in concentrations over 1%, the following were revealed: (1) Reduced pyridine nucleotide was increased, (2) oscillatory change of the reduced pyridine nucleotide, which is characteristic for the histotoxic reaction, developed, and (3) extent of the fluorescence changes tended to be in proportion to the concentration of CO, but no change was caused by inhalation of CO in lower concentrations (0.1%, 0.5%) even if the inhalation was continued for 30 min. As to the blood flow, CO in concentrations over 0.5% decreased it, whereas anoxia due to nitrogen increased it. Some implications were discussed from toxicological points of view on the results obtained.
D000818:Animals; D002248:Carbon Monoxide / Q000009:adverse effects*; D005260:Female; D005453:Fluorescence; D005470:Fluorometry; D000860:Hypoxia / Q000378:metabolism; D007668:Kidney / Q000098:blood supply / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D009584:Nitrogen / Q000009:adverse effects; D010084:Oxidation-Reduction / Q000187:drug effects*; D011817:Rabbits; D012039:Regional Blood Flow / Q000187:drug effects
D002248:Carbon Monoxide; D009584:Nitrogen