Eucaryotic elongation factors Ts is an integral component of rabbit reticulocyte elongation factor 1.
Elongation factor 1 (EF-1) was purified from rabbit reticulocytes and found to contain at least two distinct polypeptides: one of Mr 53 000 and one of Mr 30 000. The 30 000-Mr polypeptide was purified from EF-1 by treatment of the factor with 5.4 M guanidine . HCl and subsequent chromatography on DEAE-BioGel A in the presence of 5 M urea. By a number of functional criteria, the 30 000-Mr polypeptide was found to be the eucaryotic elongation factor Ts (eEF-Ts). These criteria include the ability of the polypeptide to stimulate Artemia salina eEF-Tu-dependent binding of aminoacyl-tRNA to 80-S ribosomes as well as eEF-Tu + EF-2-dependent polyphenylalanine synthesis. The reticulocyte factor also markedly increased the rate of exchange of eEF-Tu . gdp complexes with free GTP. Furthermore, rabbit antibodies to EF-1 from A. salina which was previously shown to contain eEF-Ts [Slobin, L. I. and Möller, W. (1978) Eur. J. Biochem. 84, 69--77] were found to cross-react with reticulocyte eEF-Ts, suggesting extensive structural homology between brine shrimp and rabbit eEF-Ts. The demonstration that eEF-Ts is and integral component of EF-1 from such diverse sources as brine shrimp and rabbit reticulocytes supports the conclusion that the factor is universally present in eucaryotic EF-1.
D000818:Animals; D033364:Decapoda; D006146:Guanidines; D007700:Kinetics; D046911:Macromolecular Substances; D008970:Molecular Weight; D010452:Peptide Biosynthesis; D010441:Peptide Chain Elongation, Translational; D010445:Peptide Elongation Factors / Q000032:analysis* / Q000378:metabolism; D011233:Precipitin Tests; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism; D011817:Rabbits; D012156:Reticulocytes / Q000032:analysis*; D012270:Ribosomes / Q000378:metabolism
D006146:Guanidines; D046911:Macromolecular Substances; D010445:Peptide Elongation Factors; D012346:RNA, Transfer, Amino Acyl