The specificity of multi-column liquid chromatography of neutral urinary steroids as evaluated by comparison with parallel gas chromatography and mass spectrometry.
The use of gas chromatography/mass spectrometry is advocated as the best currently available methodology for the validation of a routine assay for steroid analysis. This is documented by comparative studies by gas chromatography/mass spectrometry and spectrophotometry of peak material obtained in routine assays by multi-column liquid chromatographic techniques.
D002849:Chromatography, Gas; D002853:Chromatography, Liquid; D005069:Evaluation Studies as Topic; D006801:Humans; D013058:Mass Spectrometry; D008722:Methods; D013256:Steroids / Q000652:urine*; D013760:Tetrahydrocortisol; D013761:Tetrahydrocortisone
D013256:Steroids; D013761:Tetrahydrocortisone; D013760:Tetrahydrocortisol