Polypeptide hormone binding sites in vivo: initial localization of 125I-labeled insulin to hepatocyte plasmalemma as visualized by electron microscope radioautography.
The rationale of the specific-binding assay was applied to the detection of the liver insulin receptor in vivo. Quantitative electron microscope radioautography indicated that, 3 min after an intraportal injection, 125I-insulin was exclusively located to the hepatocyte plasmalemma.
D000818:Animals; D001345:Autoradiography; D002462:Cell Membrane / Q000378:metabolism / Q000648:ultrastructure; D007457:Iodine Radioisotopes; D008099:Liver / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D011972:Receptor, Insulin / Q000032:analysis*
D007457:Iodine Radioisotopes; D011972:Receptor, Insulin
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