Stable association of the human transgenome and host murine chromosomes demonstrated with trispecific microcell hybrids.
Trispecific microcell hybrids were prepared by transferring limited numbers of chromosomes from a human/mouse gene-transfer cell line to a Chinese hamster recipient line. The donor cells employed were murine L-cells that stably expressed the human form of the enzyme hypoxanthine phosphoribosyltransferase. Karyotypic, zymographic, and back-selection tests of the resulting human/mouse/Chinese hamster microcell hybrids provided strong genetic evidence for a stable association of the human transgenome with host murine chromosomes in stable gene-transfer cell lines. This association, which may represent physical integration of the transgenome into the host cell genome, occurred at multiple chromosomal sites.
D002460:Cell Line; D002875:Chromosomes / Q000502:physiology*; D005796:Genes; D006801:Humans; D006822:Hybrid Cells / Q000502:physiology*; D007527:Isoenzymes / Q000235:genetics; D007621:Karyotyping; D007739:L Cells / Q000502:physiology
D007527:Isoenzymes
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