Enucleation and reconstruction of interferon-producing cells.
Enucleation of L cells leads to loss of the capacity to produce interferon, showing that the cell nucleus is essential for interferon formation. However, when the cells were enucleated while interferon formation. However, when the cells were enucleated while interferon formation was proceeding, the cytoplasts were capable of continuing to synthesize interferon by a process shown to be protein synthesis, showing that the interferon messenger RNA leaves the nucleus after synthesis. Reconstructed cells were obtained by Sendai virus fusion of karyoplasts and cytoplasts. Such reconstructed cells were capable of producing at least as much interferon (43 interferon units/10(4) nucleated cells) as control cells (31 interferon units/10(4) nucleated cells).
D002467:Cell Nucleus / Q000502:physiology*; D003593:Cytoplasm / Q000378:metabolism; D007372:Interferons / Q000096:biosynthesis*; D007700:Kinetics; D007739:L Cells / Q000378:metabolism / Q000502:physiology*; D014176:Protein Biosynthesis
D007372:Interferons
J Biol Chem. 1951 Nov;193(1):265-75
14907713
J Virol. 1971 Jun;7(6):792-801
4327587
J Virol. 1971 May;7(5):588-94
4104252
Exp Cell Res. 1972;71(2):480-5
5065231
J Gen Virol. 1973 Oct;21:19-29
4796809
Proc Natl Acad Sci U S A. 1974 May;71(5):1999-2002
4525471
Proc Natl Acad Sci U S A. 1972 May;69(5):1203-7
4624755
Proc Natl Acad Sci U S A. 1974 Jun;71(6):2251-5
4526303
J Cell Physiol. 1975 Apr;85(2 Pt 2 Suppl 1):357-64
235557
J Gen Virol. 1974 Dec;25(3):381-90
4475093
Proc Natl Acad Sci U S A. 1975 Oct;72(10):3975-7
172890
Exp Cell Res. 1972 Sep;74(1):99-109
4672478
Proc Natl Acad Sci U S A. 1971 Mar;68(3):580-4
5276765
Proc Natl Acad Sci U S A. 1975 Dec;72(12):4881-5
1061077
Proc Natl Acad Sci U S A. 1975 Oct;72(10):3898-901
1060071
Proc Natl Acad Sci U S A. 1976 Feb;73(2):520-523
1061154
J Gen Virol. 1975 Feb;26(2):149-58
1167574