Anti-epidermal-cell-surface pemphigus antibody detaches viable epidermal cells from culture plates by activation of proteinase.
Immunoglobulin from pemphigus patients binds to the surface of mouse epidermal cells in culture. Cells incubated with the pemphigus antibody are easily detached from culture plates whereas cells incubated with serum from normal patients remain on the plate. Pemphigus antibody-mediated cell detachment is blocked by the addition of the proteinase inhibitors soybean trypsin inhibitor and alpha2-macroglobulin to the culture media. Detachable cells are viable, and activation of the complement cascade is not necessary for cell detachment. The anti-cell-surface antibody of pemphigus appears to disrupt adhesion between viable epidermal cells by activation of proteinase.
D000906:Antibodies; D000937:Antigen-Antibody Reactions; D002448:Cell Adhesion / Q000187:drug effects; D002470:Cell Survival; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D004789:Enzyme Activation; D004817:Epidermis / Q000201:enzymology / Q000276:immunology*; D006801:Humans; D007140:Immunoglobulin Fab Fragments; D010392:Pemphigus / Q000276:immunology*; D011480:Protease Inhibitors
D000906:Antibodies; D007140:Immunoglobulin Fab Fragments; D011480:Protease Inhibitors; D003208:Concanavalin A
JAMA. 1965 May 24;192:682-8
14280515
Proc Soc Exp Biol Med. 1964 Nov;117:505-10
14233481
J Invest Dermatol. 1977 May;68(5):259-64
67155
Arch Dermatol. 1977 Jul;113(7):923-6
406854
J Invest Dermatol. 1977 May;68(5):265-71
404362
Science. 1977 Dec 16;198(4322):1162-3
929193
Nature. 1977 Oct 6;269(5628):521-2
909603
Biochim Biophys Acta. 1976 Dec 8;452(2):458-67
1009122
J Immunol. 1972 May;108(5):1146-51
4112916
Vox Sang. 1972 Jul-Aug;23(1):119-25
4628154
Blood. 1972 Jan;39(1):1-12
4550467
Proc Natl Acad Sci U S A. 1973 Apr;70(4):1012-6
4515602
J Invest Dermatol. 1971 Jun;56(6):474-9
4996290
J Invest Dermatol. 1976 Aug;67(2):254-60
950490
J Invest Dermatol. 1976 May;66(5):292-6
1270836
J Immunol. 1975 Feb;114(2 pt 2):894-7
1167564
Anal Biochem. 1966 Apr;15(1):45-52
5959431