Synthesis of opiate peptides by a clonal pituitary tumor cell line.
Clonal mouse pituitary tumor cells, AtT-20, synthesize at least four species of peptides with opiate activity. The endorphin concentration of AtT-20 cells was estimated to be 300-600 pmol/mg of protein. The two most abundant endorphins with apparent molecular weights of 1800 and 2400 were purified 300- and 24-fold, respectively; additional minor components were found with apparent molecular weights of greater than 3000 and less than 750.
D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D001681:Biological Assay; D002460:Cell Line; D002999:Clone Cells; D004558:Electric Stimulation; D004723:Endorphins / Q000096:biosynthesis*; D004745:Enkephalins / Q000096:biosynthesis* / Q000494:pharmacology; D004789:Enzyme Activation; D006168:Guinea Pigs; D007082:Ileum / Q000187:drug effects / Q000502:physiology; D007700:Kinetics; D009270:Naloxone / Q000494:pharmacology; D009842:Oligopeptides / Q000096:biosynthesis*; D010452:Peptide Biosynthesis; D010447:Peptide Hydrolases / Q000494:pharmacology; D010911:Pituitary Neoplasms / Q000378:metabolism; D011458:Prostaglandins E / Q000494:pharmacology
D004723:Endorphins; D004745:Enkephalins; D009842:Oligopeptides; D011458:Prostaglandins E; D009270:Naloxone; D010447:Peptide Hydrolases; D000262:Adenylyl Cyclases
Proc Natl Acad Sci U S A. 1962 Jul 15;48:1184-90
13874682
Nature. 1975 Dec 18;258(5536):577-80
1207728
J Biol Chem. 1951 Nov;193(1):265-75
14907713
Biochem Biophys Res Commun. 1977 Jan 24;74(2):656-62
189770
Nature. 1976 Oct 14;263(5578):609-12
185523
J Pharm Pharmacol. 1976 Aug;28(8):660-1
11324
Brain Res. 1976 Oct 15;115(2):285-96
10049
Proc Natl Acad Sci U S A. 1976 Nov;73(11):3942-6
1069261
J Clin Endocrinol Metab. 1974 Oct;39(4):684-92
4137932
Nature. 1965 Dec 11;208(5015):1093-4
4162144
Endocrinology. 1973 Feb;92(2):385-93
4345532
Brain Res. 1975 May 2;88(2):295-308
1148827
Am Zool. 1968 May;8(2):285-305
4312019
Proc Natl Acad Sci U S A. 1975 Feb;72(2):590-4
1054841
Genetics. 1975 Jun;79 Suppl:373-83
1150088
Eur J Pharmacol. 1975 Sep-Oct;33(2):395-9
241656
Proc Natl Acad Sci U S A. 1974 Sep;71(9):3474-7
4530316
Proc Natl Acad Sci U S A. 1976 Jul;73(7):2515-9
1065904
Proc Natl Acad Sci U S A. 1976 Apr;73(4):1145-8
1063395
Cancer Res. 1959 Jan;19(1):72-8
13618887