Mechanisms of inactivation of bacteriophage phiX174 and its DNA in aerosols by ozone and ozonized cyclohexene.
The mechanisms of inactivation of aerosolized bacteriophage phiX174 in atmospheres containing ozone, cyclohexene, or ozonized cyclohexene were studied by using 32P-labelled phage. The inactivation of the aerosolized phage in clear air or in air containing cyclohexene is due to damage of the protein coat since the deoxyribonucleic acid (DNA) extracted from the inactivated phage retains its biological activity. Inactivation of the phage in air containing ozonized cyclohexene is due both to protein and DNA damage. Sucrose gradient analysis shows that aerosolized inactivated phiX174 releases unbroken DNA. In contrast, the DNA from phage phiX174 inactivated by ozonized cyclohexene is broken. The inactivation of aerosolized phage phiX174-DNA was studied in the same atmospheres using 32P-labelled DNA. phiX174-DNA aerosolized in clear air or air containing cyclohexene at 75% r.h. is inactivated by a factor of 2 in 30 min. The inactivated DNA is broken. Ozone as well as ozonized cyclohexene inactivates KNA very fast causing breaks in the molecule. This is in contrast with the intact bacteriophage in which ozone does not produce breaks in the DNA.
D000336:Aerosols; D000475:Alkenes / Q000494:pharmacology*; D001272:Atmosphere; D002499:Centrifugation, Density Gradient; D003090:Coliphages / Q000187:drug effects*; D004267:DNA Viruses / Q000187:drug effects; D004279:DNA, Viral; D010126:Ozone / Q000494:pharmacology*
D000336:Aerosols; D000475:Alkenes; D004279:DNA, Viral; D010126:Ozone
Int J Air Pollut. 1959 Oct;2:167-74
13794387
J Hyg (Lond). 1977 Apr;78(2):189-98
265341
J Hyg (Lond). 1977 Apr;78(2):175-87
321676
Arch Environ Health. 1971 Aug;23(2):149-53
4397679
Appl Microbiol. 1972 May;23(5):938-41
4555637
Nature. 1968 Oct 26;220(5165):395-6
4879332
J Hyg (Lond). 1970 Jun;68(2):245-52
4914088
Ann Inst Pasteur (Paris). 1968 May;114(5):595-607
4938484
Atmos Environ. 1969 Nov;3(6):669-82
5382204
Anal Biochem. 1969 Oct 15;32(1):14-30
5398260
Bacteriol Rev. 1966 Sep;30(3):559-70
5917336
Radiat Res. 1967 Mar;30(3):468-82
6022206
Arch Biochem Biophys. 1954 Jul;51(1):208-16
13181475