Cytopathologic effects of chlorhexidine on human cells.
Exposure of human cells in culture to chlorhexidine at concentrations equal to or greater than 0.004% resulted in impaired cellular function and/or cell death. Release of membrane bound 51Cr, inhibition of protein synthesis as measured by incorporation of 3H-leucine into protein-like material, and staining by trypan blue were seen as sequellae to exposure to 0.006% chlorhexidine for 3 hours. Lower doses were capable of inhibiting protein synthesis and releasing 51Cr, but did not result in staining of cells by trypan blue. Exposure of cells to 0.2% chlorhexidine for 30 seconds produced maximal suppression of protein synthesis and release of 51Cr.
D000596:Amino Acids / Q000378:metabolism; D001645:Biguanides / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002710:Chlorhexidine / Q000008:administration & dosage / Q000494:pharmacology*; D002857:Chromium / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D005881:Gingiva / Q000187:drug effects / Q000502:physiology; D006367:HeLa Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D014945:Wound Healing / Q000187:drug effects
D000596:Amino Acids; D001645:Biguanides; D002857:Chromium; D002710:Chlorhexidine