Authentic beta-globin mRNA sequences in homozygous betaO-thalassemia.
In a patient with homozygous betaO-thalassemia in whom studies of reticulocyte hemoglobin synthesis showed no beta-globin chain synthesis in vivo and in vitro, molecular hybridization studies revealed RNA sequences complementary to beta-globin cDNA. The fact that these sequences were authentic beta-globin mRNA was shown by fingerprint analysis of T1 ribonuclease-digested mRNA and by sequencing of oligonucleotides unique to beta-globin mRNA. The beta-mRNA that failed to direct beta-globin chain synthesis was not detectably shortened or degraded and contained poly(A) sequences.
D000328:Adult; D001483:Base Sequence; D005796:Genes; D005914:Globins / Q000096:biosynthesis; D006720:Homozygote; D006801:Humans; D008297:Male; D011061:Poly A / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism*; D013789:Thalassemia / Q000235:genetics / Q000378:metabolism*
D012333:RNA, Messenger; D011061:Poly A; D005914:Globins
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