title: Kinetics of processing of type I and type III procollagens in fibroblast cultures.
abstract: The rates of conversion of types I and III procollagens to their respective collagens were compared in seven fibroblast culture systems of murine or human origin. During 24 hr of radioactive labeling or after shorter pulses followed by chases of 20-48 hr, no evidence was obtained for conversion of radiolabeled type III procollagen to insoluble collagen. In the same cultures and under the same conditions, native collagen was generated from radiolabeled type I procollagen. There was no evidence for proteolytic degradation of type III procollagen during the chase experiments. The results are ascribed to a lack of availability of the enzymes required for processing of type III procollagen.
mesh_terms: D002460:Cell Line; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis*; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D008970:Molecular Weight; D011347:Procollagen / Q000378:metabolism*
chemical_list: D011347:Procollagen; D003094:Collagen
references.citation: Biochemistry. 1977 Jun 28;16(13):2980-5
references.pmid: 195597
references.citation: Cell. 1977 May;11(1):169-72
references.pmid: 559546
references.citation: Mol Cell Biochem. 1976 Dec 10;13(3):165-92
references.pmid: 1004502
references.citation: Hoppe Seylers Z Physiol Chem. 1975 Nov;356(11):1783-92
references.pmid: 1107200
references.citation: Biochem Biophys Res Commun. 1975 Oct 27;66(4):1160-5
references.pmid: 1191284
references.citation: Nat New Biol. 1973 Aug 8;244(136):188-90
references.pmid: 4353422
references.citation: Proc Natl Acad Sci U S A. 1973 Feb;70(2):361-5
references.pmid: 4346885
references.citation: Cell. 1975 Jan;4(1):45-50
references.pmid: 1167812
references.citation: Biochemistry. 1974 Dec 3;13(25):5243-8
references.pmid: 4611481
references.citation: Biochemistry. 1974 Aug 13;13(17):3459-64
references.pmid: 4602296
references.citation: J Invest Dermatol. 1975 Jul;65(1):85-92
references.pmid: 50387
references.citation: J Biol Chem. 1974 May 25;249(10):3225-31
references.pmid: 4598122
references.citation: Proc Natl Acad Sci U S A. 1972 Dec;69(12):3655-9
references.pmid: 4509328
references.citation: Eur J Biochem. 1974 Jul 1;46(1):83-8
references.pmid: 4850204
references.citation: J Mol Biol. 1973 Sep 15;79(2):237-48
references.pmid: 4760132
references.citation: Biochem Biophys Res Commun. 1975 Feb 17;62(4):946-52
references.pmid: 1091269
references.citation: Biochim Biophys Acta. 1975 Jul 21;400(1):121-31
references.pmid: 1096957
references.citation: Biochemistry. 1975 Apr 22;14(8):1589-94
references.pmid: 1092340
references.citation: Arch Biochem Biophys. 1976 Apr;173(2):490-4
references.pmid: 1275503
references.citation: Biochemistry. 1975 Nov 18;14(23):5188-94
references.pmid: 1238109
references.citation: J Biol Chem. 1975 Dec 25;250(24):9304-12
references.pmid: 1194285
references.citation: Biochim Biophys Acta. 1975 Oct 20;405(2):412-21
references.pmid: 1180964