title: Growth and maintenance of HeLa cells in serum-free medium supplemented with hormones.
abstract: HeLa cells grow in a nutritionally complete synthetic medium (Ham's F12) supplemented with insulin, transferrin, hydrocortisone (aldosterone), fibroblast growth factor, and epidermal growth factor. This hormone-supplemented medium supports clonal growth, long-term cultivation, and a growth rate equal to that of serum-supplemented medium. The omission of any one of the five components results in less than maximal cell growth.
mesh_terms: D003470:Culture Media; D006133:Growth Substances; D006367:HeLa Cells; D006728:Hormones
chemical_list: D003470:Culture Media; D006133:Growth Substances; D006728:Hormones
references.citation: Proc Natl Acad Sci U S A. 1955 Jul 15;41(7):432-7
references.pmid: 16589695
references.citation: J Biol Chem. 1959 Oct;234:2754-8
references.pmid: 14416837
references.citation: Science. 1955 Sep 16;122(3168):501-14
references.pmid: 13255879
references.citation: Proc Natl Acad Sci U S A. 1965 Feb;53:288-93
references.pmid: 14294058
references.citation: Exp Cell Res. 1964 Dec;36:439-74
references.pmid: 14242231
references.citation: J Lab Clin Med. 1957 Jun;49(6):825-36
references.pmid: 13429201
references.citation: J Exp Med. 1955 Jul 1;102(1):37-48
references.pmid: 14392239
references.citation: Exp Cell Res. 1971 Aug;67(2):295-304
references.pmid: 4328993
references.citation: Nat New Biol. 1972 Apr 12;236(67):180-1
references.pmid: 4260653
references.citation: Proc Natl Acad Sci U S A. 1973 Sep;70(9):2702-6
references.pmid: 4354860
references.citation: Recent Prog Horm Res. 1974;30(0):533-50
references.pmid: 4601978
references.citation: Acta Endocrinol Suppl (Copenh). 1973;175:1-35
references.pmid: 4542208
references.citation: Nature. 1976 Jan 15;259(5539):132-4
references.pmid: 813153
references.citation: Proc Natl Acad Sci U S A. 1976 Jun;73(6):2023-7
references.pmid: 1064872
references.citation: J Biol Chem. 1975 Apr 10;250(7):2515-20
references.pmid: 1168187
references.citation: J Cell Sci. 1969 Jul;5(1):135-42
references.pmid: 5353651