title: Microinterferometric measurement of dry weight of blood lymphocytes in hematologically normal patients and in patients with chronic lymphocytic leukemia.
abstract: Lymphocytes from the peripheral blood of 20 patients with nonhematologic disorders (HN patients) and 13 patients with chronic lymphocytic leukemia (CLL) were studied. A scanning and integrating microinterferometer was used for calculation of dry weight of cells. In HN patients dry weight of lymphocytes was found to be 38.09 +/- 11.11 pg. The HN patients could be divided into three groups according to their lymphocytes' mund to be 29.79 +/- 7.99 pg. This population was uniform and resembled the "light" group of lymphocytes in HN patients. A study of the kinetics of PHA transformation of lymphocytes from both HN and CLL patients revealed a good correlation between the morphological evaluation and dry weight calculations.
mesh_terms: D006801:Humans; D007368:Interferometry; D007700:Kinetics; D037102:Lectins / Q000494:pharmacology; D007945:Leukemia, Lymphoid / Q000097:blood*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000166:cytology
chemical_list: D037102:Lectins