Involvement of mitochondria in changes in fluorescein excitation and emission polarization spectra in living cells.
The comparison of fluorescein polarization spectra in living cells and in isolated subcellular structures identified the mitochondria as the cytoplasmic domain in which on excitation at 470 nm the sharp fluorescein emission polarization peak at 510 nm is formed. Changes in the emission polarization peak during the cell cycle or those induced by growth stimulators and inhibitors reflect structural changes in the mitochondria on their transition from the resting, orthodox into the active, ATP-generating, condensed conformation and vice versa. Possible mechanisms for the formation of the sharp emission polarization peak are discussed.
D000818:Animals; D002453:Cell Cycle; D005452:Fluoresceins / Q000032:analysis*; D005454:Fluorescence Polarization; D066298:In Vitro Techniques; D008930:Mitochondria, Liver / Q000032:analysis*; D051381:Rats; D011919:Rats, Inbred Strains; D013347:Subcellular Fractions / Q000032:analysis
D005452:Fluoresceins
Eur J Cancer. 1977 Sep;13(9):903-15
334557
Biophys J. 1978 Sep;23(3):395-405
698344
Br J Cancer. 1978 Jul;38(1):163-5
687515
Exp Cell Res. 1971 May;66(1):137-44
4253325
Br J Cancer. 1974 Feb;29(2):168-75
4857338
Immunology. 1975 Nov;29(5):885-91
1081487
Proc Natl Acad Sci U S A. 1966 Jan;55(1):134-41
5220862
Science. 1965 Feb 12;147(3659):729-32
14242017
Radiat Res. 1963 Sep;20:55-70
14061481
Biochem J. 1960 Nov;77:326-7
13682222
Biochem J. 1952 May;51(2):145-55
14944566