Isolation of an electrogenic K+/Ca2+ ionophore from an ionophoroprotein of beef heart mitochondria.
A K+/Ca2+ electrogenic ionophore has been isolated from an ionophoroprotein of beef heart mitochondria and identified as a neutral peptide of molecular weight 1600. The amino acid composition and cationic specificity of the ionophore have been determined. The free ionophore was released from the ionophoroprotein as a consequence of tryptic digestion. The ionophoroprotein can be converted to an ionophoro peptide (molecular weight 5,100) by proteolysis without release of the free ionophore. The isolation of a K+/Ca2+ ionophore thus provides an introduction to the general technology of extracting ionophoro proteins and ofreleasing ionophores from these proteins by proteolytic digestion.
D000818:Animals; D001693:Biological Transport, Active; D002118:Calcium / Q000378:metabolism*; D002417:Cattle; D007476:Ionophores; D007700:Kinetics; D008274:Magnesium / Q000378:metabolism; D008566:Membranes / Q000378:metabolism / Q000648:ultrastructure; D008929:Mitochondria, Heart / Q000378:metabolism*; D009124:Muscle Proteins / Q000302:isolation & purification / Q000378:metabolism; D011188:Potassium / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism
D007476:Ionophores; D009124:Muscle Proteins; D012964:Sodium; D008274:Magnesium; D011188:Potassium; D002118:Calcium
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