Sensitivity of ribosomal bound N-acetylphenylalanyl-tRNA to hydrolysis by a spectific hydrolase.
Binding of N-acetylphenylalanyl-tRNA, either enzymatically or non-enzymatically, to yeast 80-S ribosomes renders the substrate resistant to the hydrolytic action of a specific hydrolase present in yeast. In contrast, N-acetylphenylalanyl-tRNA bound to the 40-S ribosomal subunit is sensitive to enzymatic hydrolysis. The presence of the hydrolase in the aminoacyl-tRNA binding factor preparations greatly interferes with the formation of complexes between N-acetylphenylalanyl-tRNA and the small ribosomal subunit.
D006867:Hydrolases / Q000378:metabolism*; D006868:Hydrolysis; D010649:Phenylalanine / Q000031:analogs & derivatives / Q000378:metabolism; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*; D012440:Saccharomyces / Q000378:metabolism
D012346:RNA, Transfer, Amino Acyl; D010649:Phenylalanine; D006867:Hydrolases