Identification of synaptic acetylcholine receptor sites in retina with peroxidase-labeled alpha-bungarotoxin.
An alpha-bungarotoxin-horseradish peroxidase conjugate, which binds specificity to nicotinic acetylcholine receptors, was synthesized. This conjugate was bound by 5-7% of the synapses in the inner plexiform layer of the chicken retina. Bipolar ribbon synapses as well as amacrine synapses bound the conjugate.
D000109:Acetylcholine / Q000378:metabolism*; D000818:Animals; D001667:Binding, Competitive; D002038:Bungarotoxins / Q000378:metabolism*; D002645:Chickens; D003964:Diaphragm / Q000378:metabolism; D005260:Female; D005399:Fishes; D006735:Horseradish Peroxidase; D051379:Mice; D008854:Microscopy, Electron; D008970:Molecular Weight; D009045:Motor Endplate / Q000378:metabolism; D011950:Receptors, Cholinergic / Q000378:metabolism; D011978:Receptors, Nicotinic / Q000378:metabolism; D012160:Retina / Q000378:metabolism*; D013569:Synapses / Q000378:metabolism / Q000648:ultrastructure
D002038:Bungarotoxins; D011950:Receptors, Cholinergic; D011978:Receptors, Nicotinic; D006735:Horseradish Peroxidase; D000109:Acetylcholine
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