Measurement of daily cholesterol synthesis rates in man by assay of the fractional conversion of mevalonic acid to cholesterol.
A significant correlation has been found in man between total daily cholesterol synthesis rates as determined by sterol balance measurements and the fraction of intravenously administered R-[5-14C]mevalonic acid converted to cholesterol. In 30 studies it was found that the mean daily cholesterol synthesis rates estimated by sterol balance measurements ranged from 395 to 3047 mg/day, whereas the fractional conversion of mevalonate to cholesterol varied from 0.28 to 0.99. The two parameters correlated with a coefficient of 0.87, P less than 0.001. This method for estimating cholesterol synthesis rates requires low doses of radioisotopic materials (25 muCi of [14C]mevalonate and 5 muCi of [3H]cholesterol) and less than 1 hr of the patient's time; it can be repeated at intervals of 3 weeks and reflects cholesterol synthesis over a short period of time.
D000328:Adult; D000368:Aged; D002784:Cholesterol / Q000096:biosynthesis* / Q000097:blood; D002792:Cholestyramine Resin / Q000494:pharmacology; D005260:Female; D006801:Humans; D007700:Kinetics; D008297:Male; D008798:Mevalonic Acid / Q000378:metabolism*; D008875:Middle Aged; D013185:Squalene / Q000378:metabolism
D002792:Cholestyramine Resin; D013185:Squalene; D002784:Cholesterol; D008798:Mevalonic Acid
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