title: Cloning specific segments of the mammalian genome: bacteriophage lambda containing mouse globin and surrounding gene sequences.
abstract: We have developed a general approach to the cloning of specific segments of the mammalian genome that involves a two-step purification of EcoRI fragments of mammalian DNA and their in vitro insertion into a suitably constructed EK2 derivative of bacteriophage lambda. The combination of fragment purification, exclusion of parental-type recombinants, and simple phage screening techniques permits the isolation of virtually any gene segment for which there is an identifying hybridization probe. We illustrate the approach by describing the cloning of an approximately 7000-base-long segment of mouse DNA containing globin and surrounding gene sequences.
mesh_terms: D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D003090:Coliphages / Q000235:genetics*; D004247:DNA / Q000302:isolation & purification; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D005796:Genes; D005914:Globins / Q000235:genetics*; D051379:Mice; D009693:Nucleic Acid Hybridization; D011995:Recombination, Genetic
chemical_list: D004274:DNA, Recombinant; D005914:Globins; D004247:DNA; D004262:DNA Restriction Enzymes
references.citation: Proc Natl Acad Sci U S A. 1962 Oct 15;48:1718-24
references.pmid: 13955956
references.citation: Gene. 1977 May;1(3-4):229-39
references.pmid: 338418
references.citation: Cell. 1977 Apr;10(4):571-85
references.pmid: 558827
references.citation: Science. 1977 Apr 8;196(4286):180-2
references.pmid: 322279
references.citation: Cell. 1976 Jun;8(2):227-32
references.pmid: 786462
references.citation: Nature. 1976 Oct 7;263(5577):526-7
references.pmid: 1067476
references.citation: Proc Natl Acad Sci U S A. 1976 Sep;73(9):3117-21
references.pmid: 1067607
references.citation: Nucleic Acids Res. 1976 Oct;3(10):2575-92
references.pmid: 995644
references.citation: Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184-8
references.pmid: 1055375
references.citation: Biochem Biophys Res Commun. 1972 Nov 1;49(3):813-9
references.pmid: 4638756
references.citation: Cold Spring Harb Symp Quant Biol. 1974;38:753-61
references.pmid: 4133990
references.citation: J Virol. 1974 Nov;14(5):1235-44
references.pmid: 4372397
references.citation: Biochemistry. 1973 Jul 31;12(16):3055-63
references.pmid: 4354250
references.citation: Proc Natl Acad Sci U S A. 1974 Nov;71(11):4579-83
references.pmid: 4216019
references.citation: Nature. 1974 Oct 11;251(5475):476-81
references.pmid: 4608939
references.citation: Proc Natl Acad Sci U S A. 1973 Nov;70(11):3240-4
references.pmid: 4594039
references.citation: Biochim Biophys Acta. 1973 Mar 23;303(1):52-60
references.pmid: 4573825
references.citation: Biochim Biophys Acta. 1973 Mar 23;303(1):61-7
references.pmid: 4702006
references.citation: Science. 1972 Nov 24;178(4063):873-4
references.pmid: 5085986
references.citation: J Mol Biol. 1966 Mar;16(1):118-33
references.pmid: 5331240
references.citation: J Mol Biol. 1975 Nov 5;98(3):551-64
references.pmid: 1104875
references.citation: Nature. 1976 Feb 19;259(5544):596-8
references.pmid: 765843
references.citation: Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961-5
references.pmid: 1105573
references.citation: Nature. 1975 Oct 23;257(5528):712-4
references.pmid: 1186850
references.citation: J Mol Biol. 1975 Nov 5;98(3):503-17
references.pmid: 1195397
references.citation: J Mol Biol. 1975 Sep 5;97(1):123-5
references.pmid: 1177312
references.citation: Biochim Biophys Acta. 1971 Feb 11;228(3):770-4
references.pmid: 5572614
references.citation: Biochem Biophys Res Commun. 1966 Jun 13;23(5):641-6
references.pmid: 5963888