Luminal membrane retrieved after exocytosis reaches most golgi cisternae in secretory cells.
Dextran was used to trace membrane retrieved from the luminal surface after induced exocytosis in secretory cells of rat lacrimal and parotid glands. Two different approaches were used: (a) isolated acini were incubated in vitro with dextran followed by stimulation with carbamylcholine (lacrimal) or isoproterenol (parotid) and (b) rats were injected with isoproterenol followed by dextran infusion into the parotid duct in vivo. The main findings were the same regardless of the gland source or experimental approach. Dextran was taken up initially via coated pits into smooth-surfaced apical vesicles. Shortly thereafter it was found in multiple cell compartments: within the stacked Golgi cisternae, in condensing vacuoles, and in lysosomes. Uptake was more rapid and uniform in vivo; dextran was seen in multiple cisternae of numerous Golgi complexes within 5 min after infusion. In acini incubated in vitro uptake into Golgi cisternae was more delayed and occurred with increasing frequency up to 60 min; also, more dextran was taken up into lysosomes, which were more numerous in vitro than in vivo. The results demonstrate that, after exocytosis, membrane is removed from the cell surface via vesicles that fuse with multiple cell compartments. The two novel findings are: (a) the demonstration that the tracer can reach most of the Golgi cisternae in a given stack and (b) the demonstration of the rapidity with which the process takes place (i.e., within 5 min). The findings imply that at least some membrane retrieved from the cell surface after exocytosis fuses with the stacked Golgi cisternae.
D000818:Animals; D002462:Cell Membrane / Q000648:ultrastructure*; D003911:Dextrans / Q000378:metabolism; D005089:Exocytosis / Q000187:drug effects; D006056:Golgi Apparatus / Q000648:ultrastructure*; D066298:In Vitro Techniques; D007545:Isoproterenol / Q000494:pharmacology; D007765:Lacrimal Apparatus / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D008247:Lysosomes / Q000648:ultrastructure; D008297:Male; D010306:Parotid Gland / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure*; D051381:Rats
D003911:Dextrans; D007545:Isoproterenol
Biochem J. 1956 Feb;62(2):315-23
13293190
J Cell Biol. 1976 Mar;68(3):665-87
1030706
J Cell Biol. 1977 Apr;73(1):1-13
856827
J Cell Biol. 1969 Jun;41(3):753-73
5768873
J Cell Biol. 1972 May;53(2):365-92
4112540
J Cell Biol. 1973 Feb;56(2):540-58
4345556
J Cell Biol. 1973 May;57(2):499-524
4348791
J Cell Biol. 1973 May;57(2):315-44
4348786
J Cell Biol. 1972 Jan;52(1):211-9
4331299
J Ultrastruct Res. 1973 Jun;43(5):445-59
4352596
Am J Anat. 1971 Feb;130(2):141-57
4251117
J Cell Biol. 1974 Dec;63(3):883-903
4612049
Science. 1973 Aug 10;181(4099):561-2
4578606
J Histochem Cytochem. 1973 Apr;21(4):349-85
4574997
J Cell Biol. 1976 Aug;70(2 pt 1):294-303
181385
Proc Natl Acad Sci U S A. 1976 Aug;73(8):2781-7
183210
Cell Tissue Res. 1974;156(1):1-20
4455372
Brain Res. 1972 Feb 11;37(1):141-5
5060888
J Cell Biol. 1972 May;53(2):290-311
5025103
Science. 1975 Aug 1;189(4200):347-58
1096303
J Cell Biol. 1976 Sep;70(3):692-706
956271
J Supramol Struct. 1976;4(4):527-48
933539
J Cell Biol. 1976 Jul;70(1):59-74
932102
Exp Cell Res. 1975 Sep;94(2):426-31
1238268
Cell Tissue Res. 1975 Nov 12;163(3):373-82
1203954
Biochim Biophys Acta. 1975 Jan 14;375(1):87-105
1111576
Nature. 1971 Jul 30;232(5309):341-2
5094841
J Cell Biol. 1967 Nov;35(2):357-76
6055991