title: Cleavage specificity of the restriction endonuclease isolated from Haemophilus gallinarum (Hga I).
abstract: The nucleotide sequences in the replicative form (duplex) of phiX174 DNA around six sites cut by Hga I, a restriction endonuclease from Haemophilus gallinarum, have been compared. The enzyme produces a staggered cleavage resulting in a pentanucleotide 5'-terminal extension. The sequences within and immediately surrounding the pentanucleotide cleavage site have no obvious relationship. However, the sequence 5'-G-A-C-G-C-3'  3'-C-T-G-C-G-5' occurs five nucleotide pairs to the left of the cut in the upper strand and 10 nucleotide pairs to the left of the cut in the lower strand and, therefore, is believed to constitute the recognition site. This is a member of the class of restriction endonucleases in which recognition and cleavage sites lack 2-fold rotational symmetry. The method used to define the cleavage site is of general applicability.
mesh_terms: D001483:Base Sequence; D003090:Coliphages; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004279:DNA, Viral; D003854:Deoxyribonucleotides / Q000032:analysis; D004720:Endonucleases; D006190:Haemophilus / Q000201:enzymology*; D013045:Species Specificity; D013379:Substrate Specificity
chemical_list: D004279:DNA, Viral; D003854:Deoxyribonucleotides; D004720:Endonucleases; D004262:DNA Restriction Enzymes
references.citation: Nucleic Acids Res. 1976 Oct;3(10):2665-75
references.pmid: 136644
references.citation: Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4
references.pmid: 265521
references.citation: Nature. 1977 Feb 24;265(5596):687-95
references.pmid: 870828
references.citation: CRC Crit Rev Biochem. 1976 Nov;4(2):123-64
references.pmid: 795607
references.citation: Eur J Biochem. 1976 Sep;68(1):55-70
references.pmid: 786638
references.citation: Proc Natl Acad Sci U S A. 1976 Feb;73(2):293-7
references.pmid: 1061131
references.citation: J Mol Biol. 1973 Dec 15;81(3):419-23
references.pmid: 4588280
references.citation: FEBS Lett. 1973 Aug 15;34(2):318-22
references.pmid: 4542920
references.citation: J Mol Biol. 1975 Jan 5;91(1):121-3
references.pmid: 171410
references.citation: J Biol Chem. 1971 May 25;246(10):3269-76
references.pmid: 5314021
references.citation: J Mol Biol. 1975 Jan 25;91(3):315-28
references.pmid: 1102702
references.citation: J Mol Biol. 1975 May 25;94(3):441-8
references.pmid: 1100841
references.citation: Nucleic Acids Res. 1976 May;3(5):1323-9
references.pmid: 781625
references.citation: Virology. 1976 Sep;73(2):561-7
references.pmid: 960577
references.citation: FEBS Lett. 1976 Jun 15;65(3):284-7
references.pmid: 955064