Dependence of the differentiated state on the cellular environment: modulation of collagen synthesis in tendon cells.
In an adequate environment, primary avian tendon cells are capable of retaining both the full expression of differentiated function and a correct morphological orientation for 1 week in culture. At high density and in the presence of ascorbate, they are fully stabilized in that they devote 25-30% of their total protein synthesis to collagen, a level comparable to that in tendon cells in ovo. However, either at low density or in medium without ascorbate, they synthesize collagen at only a third of this level. If plated on a collagen matrix, these cells will orient themselves in a manner similar to that of tendon cells in vivo. Furthermore, they are capable of fully modulating the percentage of collagen synthesis upon addition or removal of ascorbate and serum. The variation in the percentage of collagen produced is a result of alterations in collagen synthesis rather than of changes in total protein synthesis or hydroxylation of proline in collagen. Primary avian tendon cells, therefore, provide a suitable model for understanding the stability of the differentiated state, the mechanism of action of ascorbate, and the regulation of collagen biosynthesis.
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D002642:Chick Embryo; D003094:Collagen / Q000096:biosynthesis*; D014176:Protein Biosynthesis; D013710:Tendons / Q000166:cytology / Q000378:metabolism*; D013997:Time Factors
D003094:Collagen; D001205:Ascorbic Acid
Proc Natl Acad Sci U S A. 1965 Feb;53:288-93
14294058
Adv Genet. 1964;12:143-280
14278624
Science. 1965 Apr 2;148(3666):42-51
14258726
Nature. 1964 Oct 24;204:347-9
14228868
J Cell Physiol. 1976 NOV;89(3):355-67
185226
Exp Cell Res. 1976 Oct 1;102(1):63-71
976346
Nature. 1966 Nov 5;212(5062):631-3
5971697
Arch Biochem Biophys. 1972 Sep;152(1):318-28
4342111
Proc Natl Acad Sci U S A. 1965 Jun;53(6):1360-5
5324618
Cell. 1974 Nov;3(3):291-9
4373172
Proc Natl Acad Sci U S A. 1974 Jan;71(1):50-1
4359330
J Natl Cancer Inst. 1972 Aug;49(2):555-65
4342619
Dev Biol. 1972 Jul;28(3):443-53
4339519
Proc Natl Acad Sci U S A. 1973 Apr;70(4):1090-3
4352224
Biochemistry. 1971 Mar 16;10(6):988-94
4323854
Ann N Y Acad Sci. 1975 Sep 30;258:288-306
173226
Ann N Y Acad Sci. 1975 Sep 30;258:264-77
173224
Biochem Biophys Res Commun. 1972 Dec 4;49(5):1343-50
4645547