Repair of depurinated DNA in vitro by enzymes purified from human lymphoblasts.
Alkali-labile lesions introduced into T7 DNA by treatment with methyl methanesulfonate were removed and the DNA was repaired by incubation with DNA polymerase alpha and nuclease from a human lymphoblastoid line followed by the addition of DNA ligase. The nuclease preparation contains both apurinic endonuclease and 5'-3' exonuclease activities. Dinucleotides appear to be the first product of exonuclease action. Repair of methyl methanesulfonate-induced damage can occur by the insertion of only a few nucleotides per lesion as in vivo.
D002051:Burkitt Lymphoma / Q000201:enzymology; D002460:Cell Line; D003090:Coliphages / Q000378:metabolism; D004260:DNA Repair; D004279:DNA, Viral / Q000378:metabolism*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism; D008025:Ligases / Q000378:metabolism; D008741:Methyl Methanesulfonate / Q000494:pharmacology
D004279:DNA, Viral; D008741:Methyl Methanesulfonate; D004259:DNA-Directed DNA Polymerase; D003851:Deoxyribonucleases; D008025:Ligases
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