title: Evidence for pro-beta-nerve growth factor, a biosynthetic precursor to beta-nerve growth factor.
abstract: The biosynthesis of beta-nerve growth factor (betaNGF) was studied in mouse submaxillary glands incubated with L-[(35)S]cystine. betaNGF was isolated from tissue extracts by the addition of antiserum against betaNGF and the washed immunoprecipitates were analyzed by sodium dodecyl sulfate gel electrophoresis. With short labeling periods (10 and 25 min) there is a major labeled species with an apparent molecular weight of 22,000 and a smaller peak comigrating with purified betaNGF chains (13,260). As time proceeds, the radioactivity in the 22,000 molecular weight peak plateaus, while the label in betaNGF continues to increase, until by 4 hr it greatly exceeds the radioactivity of the 22,000 molecular weight species. When glands incubated for 10 min are transferred to medium containing a large excess of unlabeled L-cystine, the 22,000 molecular weight peak gradually declines, and there is a corresponding increase in radioactivity at the betaNGF position. The 22,000 molecular weight species isolated from sodium dodecyl sulfate gels possesses all the cystine-containing peptides of betaNGF, and possibly two additional ones. When immunoprecipitates from submaxillary glands labeled for 25 min are incubated with the gamma subunit (a specific arginyl-esteropeptidase associated with betaNGF in the 7S NGF complex), the radioactivity in the 22,000 molecular weight species is converted to the betaNGF position. The results suggest that the 22,000 molecular weight species is a biosynthetic precursor to betaNGF, and that the gamma subunit may function as a specific protease in the processing event.
mesh_terms: D000818:Animals; D007700:Kinetics; D008297:Male; D051379:Mice; D008970:Molecular Weight; D009414:Nerve Growth Factors / Q000096:biosynthesis*; D011233:Precipitin Tests; D011498:Protein Precursors / Q000378:metabolism*; D013363:Submandibular Gland / Q000378:metabolism*
chemical_list: D009414:Nerve Growth Factors; D011498:Protein Precursors
references.citation: Life Sci. 1974 Oct 15;15(8):1381-91
references.pmid: 4620979
references.citation: Biochemistry. 1976 Dec 14;15(25):5543-52
references.pmid: 999827
references.citation: J Biol Chem. 1971 Jul 25;246(14):4504-9
references.pmid: 5106387
references.citation: Proc Natl Acad Sci U S A. 1968 Aug;60(4):1383-8
references.pmid: 5244746
references.citation: Nat New Biol. 1972 Sep 27;239(91):117-20
references.pmid: 4507519
references.citation: Proc Natl Acad Sci U S A. 1973 Dec;70(12):3884-8
references.pmid: 4359493
references.citation: Physiol Chem Phys. 1975;7(1):7-21
references.pmid: 1129379
references.citation: J Biol Chem. 1976 Jan 10;251(1):165-73
references.pmid: 811664
references.citation: Proc Natl Acad Sci U S A. 1974 Sep;71(9):3731-5
references.pmid: 4530333
references.citation: Biochemistry. 1974 Feb 12;13(4):833-40
references.pmid: 4811070
references.citation: J Biol Chem. 1972 Dec 10;247(23):7612-21
references.pmid: 4636327
references.citation: Proc Natl Acad Sci U S A. 1970 Sep;67(1):164-71
references.pmid: 4989828
references.citation: Proc Natl Acad Sci U S A. 1971 Oct;68(10):2417-20
references.pmid: 4944623
references.citation: Physiol Rev. 1968 Jul;48(3):534-69
references.pmid: 4875350
references.citation: Proc Natl Acad Sci U S A. 1976 Apr;73(4):1179-83
references.pmid: 1063399
references.citation: Proc Natl Acad Sci U S A. 1976 Jun;73(6):1964-8
references.pmid: 778852
references.citation: Proc Natl Acad Sci U S A. 1975 Mar;72(3):777-9
references.pmid: 1055377
references.citation: Proc Natl Acad Sci U S A. 1976 Jan;73(1):29-33
references.pmid: 1061124
references.citation: J Biol Chem. 1976 Feb 25;251(4):1188-96
references.pmid: 1249071
references.citation: J Neurochem. 1975 Dec;25(6):843-51
references.pmid: 1206401
references.citation: Biochemistry. 1971 Feb 2;10(3):463-9
references.pmid: 5543976
references.citation: Nature. 1970 Aug 15;227(5259):680-5
references.pmid: 5432063
references.citation: Exp Cell Res. 1969 Sep;57(1):95-103
references.pmid: 5810933
references.citation: Biochemistry. 1969 Sep;8(9):3735-41
references.pmid: 5820664
references.citation: Biochemistry. 1968 Sep;7(9):3259-68
references.pmid: 5684347
references.citation: Biochemistry. 1968 Apr;7(4):1296-303
references.pmid: 5677819
references.citation: Biochemistry. 1967 Jul;6(7):2202-9
references.pmid: 6049455
references.citation: Science. 1967 Jun 2;156(3779):1253-5
references.pmid: 6025549