Coordination of ribosome content and polysome formation during estradiol stimulation of vitellogenin synthesis in immature male chick livers.
To elucidate the mechanisms by which protein synthesis is affected by estradiol, we characterized cockerel liver polysomal profiles during hormone induction and withdrawal. We describe a method for isolating intact polysomes which results in preparations that are stable even after storage in solution at 10 degrees for 16 hr. In addition, our procedure eliminates the necessity for starving animals prior to experiments. Recovery of radioactive polysomes indicated that yield is about 90% and that our polysomal preparations appear to represent polysome distribution in vivo. Using this approach we show that estradiol injection stimulates ribosome content 6-fold and that formation of polysomes is coincident with the induction of vitellogenin synthesis. We also demonstrate that the size and number of polysomes increase and decrease in a coordinated fashion with the rate of vitellogenin synthesis. The kinetics of ribosome synthesis and the fact that at least 80% of the newly synthesized ribosomes are directly recruited into polysomes indicate that ribosomes might be limiting the rate of protein synthesis during the stimulatory phase of the hormone cycle.
D000818:Animals; D002458:Cell Fractionation; D002645:Chickens; D004958:Estradiol / Q000494:pharmacology*; D008074:Lipoproteins / Q000096:biosynthesis*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D010750:Phosphoproteins / Q000096:biosynthesis; D011132:Polyribosomes / Q000187:drug effects / Q000502:physiology*; D014176:Protein Biosynthesis / Q000187:drug effects; D012260:Ribonucleases; D012269:Ribosomal Proteins / Q000096:biosynthesis; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism*; D014819:Vitellogenins / Q000096:biosynthesis*
D008074:Lipoproteins; D010750:Phosphoproteins; D012269:Ribosomal Proteins; D014819:Vitellogenins; D004958:Estradiol; D012260:Ribonucleases
Biochem J. 1963 Jun;87:571-6
13963649
J Biol Chem. 1964 Jun;239:2079-82
14213399
J Biol Chem. 1974 Sep 25;249(18):5939-47
4370392
Proc Natl Acad Sci U S A. 1975 Sep;72(9):3364-8
1059123
J Mol Biol. 1976 Jan 15;100(2):115-26
1255710
Eur J Biochem. 1976 Feb 16;62(2):299-306
1253792
Biochim Biophys Acta. 1971 Mar 25;232(3):524-8
5103129
J Biol Chem. 1975 Dec 10;250(23):9060-6
811661
Proc Natl Acad Sci U S A. 1976 May;73(5):1442-6
1064017
Biochim Biophys Acta. 1976 Jun 2;435(1):91-4
1276205
Biochim Biophys Acta. 1976 Jun 2;435(1):82-90
1276204
Can J Biochem. 1968 Aug;46(8):953-9
5672874
Biochemistry. 1969 Dec;8(12):4926-35
5391864
Biochemistry. 1974 Aug 13;13(17):3606-15
4858491
J Biol Chem. 1965 Apr;240:1687-91
14285508
Eur J Biochem. 1973 Feb 15;33(1):71-80
4734860
FEBS Lett. 1972 Aug 1;24(2):197-200
11946671
J Biol Chem. 1973 Sep 25;248(18):6300-7
4730323
Biochim Biophys Acta. 1973 Feb 4;294(1):497-506
4705998