Regulation of growth hormone messenger RNA by thyroid and glucocorticoid hormones.
Thyroid and glucocorticoid hormones stimulate growth hormone synthesis in cultured rat pituitary cells (GC). We have compared changes in growth hormone production and mRNA in these cells. Triiodothyronine (10 nM) and dexamethasone (1 micron) stimulated increases in growth hormone production by 2.5- and 3.8-fold, respectively. There were corresponding increases in the capacity of RNA from hormone-treated cells to direct synthesis of pregrowth hormone in a wheat germ cell-free translation system, suggesting hormone-regulated increases in growth hormone mRNA. Hormone-induced changes in mRNA were also demonstrated by determining the kinetics of hybridization of a cDNA probe prepared from RNA enriched (about 70%) for growth hormone translational activity with RNA from control and hormone-treated cells. These results suggest that thyroid and glucocorticoid hormones can regulate growth hormone production by influencing the levels of its mRNA.
D000937:Antigen-Antibody Reactions; D002460:Cell Line; D002474:Cell-Free System; D003907:Dexamethasone / Q000494:pharmacology; D013006:Growth Hormone / Q000096:biosynthesis* / Q000276:immunology; D007700:Kinetics; D009693:Nucleic Acid Hybridization; D010902:Pituitary Gland / Q000378:metabolism; D011061:Poly A / Q000378:metabolism; D011498:Protein Precursors / Q000096:biosynthesis* / Q000276:immunology; D012333:RNA, Messenger / Q000378:metabolism*; D014284:Triiodothyronine / Q000494:pharmacology*
D011498:Protein Precursors; D012333:RNA, Messenger; D014284:Triiodothyronine; D011061:Poly A; D003907:Dexamethasone; D013006:Growth Hormone
Proc Natl Acad Sci U S A. 1976 Oct;73(10):3369-73
185609
Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12
4504350
J Biol Chem. 1973 Mar 25;248(6):2095-106
4690593
Proc Natl Acad Sci U S A. 1973 Aug;70(8):2330-4
4525168
Acta Endocrinol Suppl (Copenh). 1973;180:357-79
4136136
Cell. 1975 Apr;4(4):367-78
47272
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3488-92
4357872
Proc Natl Acad Sci U S A. 1975 May;72(5):1787-91
168576
FEBS Lett. 1976 May 1;64(2):396-9
58797
J Biol Chem. 1973 Aug 10;248(15):5499-501
4588684
J Biol Chem. 1975 May 10;250(9):3330-6
47326
Proc Natl Acad Sci U S A. 1973 Apr;70(4):1218-21
4515620
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3646-9
4519652
Biochem Biophys Res Commun. 1975 Feb 3;62(3):577-83
164184
Sci Am. 1976 Feb;234(2):32-43
174193
Biochem Biophys Res Commun. 1974 Jul 10;59(1):420-8
4858353
J Clin Invest. 1974 Mar;53(3):768-77
4812438
J Biol Chem. 1973 Oct 25;248(20):7066-72
4743513
Gen Comp Endocrinol. 1967 Oct;9(2):217-26
4964229
Biochemistry. 1975 Jun 3;14(11):2440-6
806298
J Immunol. 1975 Dec;115(6):1617-24
1102604
Proc Natl Acad Sci U S A. 1975 Jul;72(7):2555-8
1058473
Proc Natl Acad Sci U S A. 1976 Jan;73(1):29-33
1061124
Biochemistry. 1976 Mar 9;15(5):1031-6
1252424
J Cell Biol. 1970 Oct;47(1):61-70
5513559
Gen Comp Endocrinol. 1970 Dec;15(3):432-52
5500426
Nature. 1970 Aug 15;227(5259):680-5
5432063
Endocrinology. 1966 Aug;79(2):275-80
5921766
Nucleic Acids Res. 1974 Nov;1(11):1385-97
10793698
J Biol Chem. 1951 Nov;193(1):265-75
14907713
Cell. 1975 Nov;6(3):299-305
212202