Glucocorticoids modulate the in vitro development of the embryonic rat pancreas.
The effect of the glucocorticoid analogue, dexamethasone, on the development of the embryonic pancreas was studied in tissue culture. It specifically enhances the accumulation of exocrine enzymes without altering the level of general cell proteins. The enhancement, however, is not symmetrical: the cellular levels of the two major exocrine products, amylase and chymotrypsinogen, are increased about 10- and 2-fold, respectively. Two other zymogens that are present in minor quantities, procarboxypeptidases A and B, are also increased, whereas no effect is seen on lipase A. Coordinate with these effects on synthesis, there is a dramatic change in the morphology of dexamethasone-stimulated acinar cells. Their number of zymogen granules is higher and crystalline arrays are found in the rough endoplasmic reticulum. Dexamethasone also inhibits cell replication, perhaps by selectively inhibiting the last cell divisions of the culture period. At the same time, there is a disproportionate reduction in the insulin content of cultured rudiments. We find that pancreatic development is normal in the absence of dexamethasone and that this glucocorticoid does not precociously induce the appearance of the specific secretory products, but rather enhances by a constant degree their synthesis and accumulation. Therefore, we conclude that glucocorticoids may play a modulatory but not an inductive role in pancreatic development.
D000818:Animals; D003907:Dexamethasone / Q000494:pharmacology*; D005314:Embryonic and Fetal Development; D009924:Organ Culture Techniques; D010179:Pancreas / Q000187:drug effects / Q000196:embryology* / Q000648:ultrastructure; D051381:Rats; D011919:Rats, Inbred Strains
D003907:Dexamethasone
C R Seances Soc Biol Fil. 1964;158:828-32
14186958
Proc Natl Acad Sci U S A. 1975 Jun;72(6):2007-11
237268
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3478-82
4519640
J Biol Chem. 1970 Nov 10;245(21):5800-5
4394356
Dev Biol. 1973 Feb;30(2):307-20
4350018
J Cell Biol. 1974 Jun;61(3):688-700
4151790
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3852-6
4149435
J Biol Chem. 1974 Sep 25;249(18):5916-7
4370049
J Biol Chem. 1974 Apr 25;249(8):2424-7
4362680
Res Exp Med (Berl). 1973 Mar 14;160(1):1-20
4348813
Dev Biol. 1970 Mar;21(3):452-61
4314309
Verh Anat Ges. 1972;67:507-15
4275078
Dev Biol. 1973 May;32(1):82-91
4208023
Biochemistry. 1972 Jan 4;11(1):130-6
4621580
Biochemistry. 1972 Apr 11;11(8):1517-28
4623251
Dev Biol. 1972 Dec;29(4):468-81
4570760
Dev Biol. 1972 Dec;29(4):436-67
4570759
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1466-70
4524649
Proc Natl Acad Sci U S A. 1972 Sep;69(9):2668-72
4506789
J Biol Chem. 1975 Mar 25;250(6):2073-9
163821
Dev Biol. 1974 Aug;39(2):295-307
4851381
J Biol Chem. 1974 Jun 10;249(11):3500-9
4831225
Dev Biol. 1973 Nov;35(1):143-59
4787744
Horm Metab Res. 1973 Jan;5(1):66
4706929
Dev Biol. 1972 Nov;29(3):293-306
4652270
J Biol Chem. 1972 Jun 25;247(12):3941-52
5033395
Cell. 1975 Mar;4(3):189
1091360
J Cell Biol. 1970 Oct;47(1):235-46
5513553
J Cell Biol. 1970 Feb;44(2):310-28
5460752
Anal Biochem. 1969 Mar;27(3):473-83
5767203
Biochem Biophys Res Commun. 1968 Dec 30;33(6):984-9
5750854