title: Voltage-dependent facilitation of Ca2+ entry in voltage-clamped, aequorin-injected molluscan neurons.
abstract: Voltage-clamp experiments were performed on giant neurons of the nudibranch Anisodoris nobilis injected with the Ca-sensitive photoprotein, aequorin. Depolarization beyond -10 to +5 m V produced an aequorin signal, the amplitude of which depended on the extracellular Ca2+ concentration, the amplitude of the depolarization, and its duration. In paired pulse experiments, the amplitude of the aequorin signal produced in response to the second of two identical depolarizing pulses was larger than that produced during the first, resulting from an increased entry of Ca2+ during the second pulse. The increment in Ca conductance inferred from the augmented signal during the second pulse was independent of Ca2+ influx during the first pulse but, instead, was related to the amplitude and duration of the first pulse.
mesh_terms: D000331:Aequorin / Q000494:pharmacology* / Q000502:physiology; D000818:Animals; D001693:Biological Transport, Active; D002118:Calcium / Q000378:metabolism* / Q000494:pharmacology; D004553:Electric Conductivity; D004558:Electric Stimulation; D008974:Mollusca / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D011506:Proteins / Q000494:pharmacology*
chemical_list: D011506:Proteins; D000331:Aequorin; D002118:Calcium
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