title: Turnover of 3H-5-hydroxytryptamine to 3H-5-hydroxyindoleacetic acid and the 3H-5-methoxyindoles in nondeprived and 24 hr food deprived rats.
abstract: Serotonin turnover in the lateral in hypothalamus (LH) was determined in nondeprived and 24 hr food deprived rats. The LH was infused with 0.5 muCi of 3H-5-hydroxytrptamine 1 hr prior to push-pull perfusion. The percentage of nCi/muCi of radioactivity was analyzed by thin layer chromatography and liquid scintillation spectrometry. There was significantly more 5-hydroxyindoleacetic acid and 5-methoxytryptamine formed in the 24 hr food deprived rats. These results indicate a faster 5-hydroxytryptamine turnover rate in the LH of 24 hr food deprived rats than in nondeprived rats.
mesh_terms: D008735:5-Methoxytryptamine / Q000096:biosynthesis*; D000818:Animals; D002855:Chromatography, Thin Layer; D005508:Food Deprivation; D006897:Hydroxyindoleacetic Acid / Q000096:biosynthesis*; D007031:Hypothalamus / Q000378:metabolism; D008297:Male; D051381:Rats; D012701:Serotonin / Q000378:metabolism*; D013997:Time Factors; D014363:Tryptamines / Q000096:biosynthesis*
chemical_list: D014363:Tryptamines; D012701:Serotonin; D008735:5-Methoxytryptamine; D006897:Hydroxyindoleacetic Acid