DNA sequencing with chain-terminating inhibitors.
A new method for determining nucleotide sequences in DNA is described. It is similar to the "plus and minus" method [Sanger, F. & Coulson, A. R. (1975) J. Mol. Biol. 94, 441-448] but makes use of the 2',3'-dideoxy and arabinonucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase. The technique has been applied to the DNA of bacteriophage varphiX174 and is more rapid and more accurate than either the plus or the minus method.
D001483:Base Sequence; D003090:Coliphages / Q000032:analysis; D004256:DNA Polymerase I / Q000037:antagonists & inhibitors; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004279:DNA, Viral; D003854:Deoxyribonucleotides / Q000494:pharmacology; D008722:Methods
D004279:DNA, Viral; D003854:Deoxyribonucleotides; D004256:DNA Polymerase I; D004262:DNA Restriction Enzymes
J Am Chem Soc. 1965 Apr 20;87:1785-8
14289336
Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4
265521
Nature. 1977 Feb 24;265(5596):687-95
870828
J Mol Biol. 1976 Dec 15;108(3):519-33
1088827
Biochemistry. 1966 Jan;5(1):224-31
5938940
Biochemistry. 1969 Dec;8(12):4889-96
4312460
Biochemistry. 1969 Dec;8(12):4897-904
4312461
Eur J Biochem. 1972 Jun 9;27(3):554-63
4262082
J Mol Biol. 1972 Dec 28;72(2):251-88
4571077
J Virol. 1975 Apr;15(4):759-75
163918
J Mol Biol. 1975 May 25;94(3):441-8
1100841
J Am Chem Soc. 1966 Apr 5;88(7):1549-53
5914527