Specific gonadotropin binding to Pseudomonas maltophilia.
Binding of 125I-labeled human chorionic gonadotropin to Pseudomonas maltophilia is dependent on time, temperature, and pH and the binding to this procaryotic species is hormone-specific and saturable. The equilibrium dissociation constant is 2.3 X 10(-9) M. There are no cooperative interactions between binding sites (Hill coefficient, 1.05). The number of sites is estimaated as 240 fmol/100 mug of protein. NaCl and KCl, at concentrations from 1 to 10 mM, have no effect on binding. Divalent cations (Mg2+ and Ca2+) and 1 mM EDTA inhibit hormone binding. Binding is destroyed by heat or by treatment with Pronase of alpha-chymotrypsin and is increased by phospholipase C. Binding of the labeled gonadotropin is not observed with other gram-negative organisms--e.g., Escherichia coli, Pseudomonas testosteroni, Pseudomonas aeruginosa, Enterobacter aerogenes, or Enterobacter cloacae.
D001665:Binding Sites; D001667:Binding, Competitive; D006063:Chorionic Gonadotropin / Q000378:metabolism*; D002918:Chymotrypsin / Q000378:metabolism; D005640:Follicle Stimulating Hormone / Q000378:metabolism; D006358:Hot Temperature; D007700:Kinetics; D007986:Luteinizing Hormone / Q000378:metabolism; D010740:Phospholipases / Q000378:metabolism; D011189:Potassium Chloride / Q000494:pharmacology; D011402:Pronase / Q000378:metabolism; D011549:Pseudomonas / Q000254:growth & development / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology; D013696:Temperature
D006063:Chorionic Gonadotropin; D012965:Sodium Chloride; D011189:Potassium Chloride; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D010740:Phospholipases; D002918:Chymotrypsin; D011402:Pronase
J Gen Microbiol. 1961 Sep;26:81-95
14475746
J Bacteriol. 1963 Aug;86:207-11
14058942
J Gen Microbiol. 1961 Sep;26:123-32
14449786
J Biol Chem. 1951 Nov;193(1):265-75
14907713
J Bacteriol. 1972 Oct;112(1):585-92
4116755
Biochim Biophys Acta. 1972 Aug 18;279(1):194-201
4652562
J Steroid Biochem. 1973 Nov;4(6):613-21
4363616
Biochemistry. 1973 Nov 6;12(23):4609-15
4359372
Proc Natl Acad Sci U S A. 1972 Dec;69(12):3520-3
4345503
Proc Soc Exp Biol Med. 1976 Jul;152(3):408-10
59931
Trans N Y Acad Sci. 1974 Jun;36(6):569-82
4530542
J Clin Endocrinol Metab. 1973 Jan;36(1):73-80
4681888
J Gen Microbiol. 1967 Sep;48(3):391-400
4964028
Can J Microbiol. 1976 Feb;22(2):286-94
816438
Biochim Biophys Acta. 1976 Mar 25;428(1):104-12
1260012
J Gen Microbiol. 1966 May;43(2):159-271
5963505