Binding of (3H)R5020 to progesterone receptor and serum contaminants in human endometrium.
The binding of [3H]R5020 to cytosol from human endometrium was utilized to evaluate the concentration of progesterone receptor by means of agar gel electrophoresis, using D-norgestrel as cold competitor. It was observed that radioligand binds predominantly to the receptor, and that serum contaminants such as albumin and corticosteroid-binding globulin do not interfere in the assay procedure.
D001667:Binding, Competitive; D003600:Cytosol / Q000378:metabolism; D004587:Electrophoresis, Agar Gel; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D006801:Humans; D009649:Norpregnadienes / Q000378:metabolism*; D011397:Promegestone / Q000378:metabolism*; D011980:Receptors, Progesterone / Q000378:metabolism*; D014156:Transcortin / Q000378:metabolism
D009649:Norpregnadienes; D011980:Receptors, Progesterone; D014156:Transcortin; D011397:Promegestone