title: Purification of a putative precursor of globin messenger RNA from mouse nucleated erythroid cells.
abstract: Nucleated erythroid cells were incubated for 10 min in the presence of [5-3H]uridine, and the total RNA was isolated by three different extraction procedures. RNA containing globin messenger RNA sequences was purified from other cellular RNAs by selective hybridization to globin complementary DNA cellulose. Depending upon the extraction procedure employed, 0.4-0.6% of the radioactively-labeled total cellular RNA applied to the column annealed to globin complementary DNA cellulose. The annealed RNA was treated with formaldehyde and analyzed by formaldehyde/polyacrylamide gel electrophoresis. Mature globin mRNA and an RNA migrating at approximately 15 S were observed. No globin mRNA containing sequences larger than 20 S were present. The 15S RNA was partially resolved from mature globin mRNA by neutral sucrose density gradient centrifugation. The RNA isolated from the heavy region of this gradient migrated as 15 S in the formaldehyde/polyacrylamide gels and retained its ability to quantitatively anneal to globin complementary DNA cellulose. On the basis of these observations, we conclude that nucleated erythroid cells obtained from the spleens of anemic mice have a 15S RNA which contains globin mRNA sequences. The 15S RNA is not an aggregate and is a good candidate for a globin mRNA precursor.
mesh_terms: D000818:Animals; D002467:Cell Nucleus; D002846:Chromatography, Affinity; D005914:Globins / Q000096:biosynthesis*; D051379:Mice; D008970:Molecular Weight; D009694:Nucleic Acid Precursors / Q000097:blood* / Q000302:isolation & purification; D012333:RNA, Messenger / Q000097:blood* / Q000302:isolation & purification; D013154:Spleen / Q000032:analysis
chemical_list: D009694:Nucleic Acid Precursors; D012333:RNA, Messenger; D005914:Globins
references.citation: Cell. 1975 Sep;6(1):13-9
references.pmid: 1164731
references.citation: J Mol Biol. 1974 Jun 25;86(2):363-71
references.pmid: 4415788
references.citation: J Mol Biol. 1974 Sep 5;88(1):205-19
references.pmid: 4444037
references.citation: Biochemistry. 1973 Jun 5;12(12):2330-8
references.pmid: 4710584
references.citation: J Mol Biol. 1972 Feb 14;63(3):409-29
references.pmid: 5014927
references.citation: J Cell Biol. 1971 Jun;49(3):683-91
references.pmid: 4997171
references.citation: Cell. 1975 Jul;5(3):281-90
references.pmid: 807333
references.citation: Cell. 1975 Jan;4(1):11-20
references.pmid: 1090376
references.citation: Cell. 1976 Feb;7(2):227-37
references.pmid: 954080
references.citation: Cell. 1975 Feb;4(2):77-93
references.pmid: 1125979
references.citation: Anal Biochem. 1966 Feb;14(2):269-77
references.pmid: 5939865
references.citation: Proc Natl Acad Sci U S A. 1974 Oct;71(10):3892-5
references.pmid: 4139705
references.citation: Nat New Biol. 1972 Apr 12;236(67):172-4
references.pmid: 4503731
references.citation: Biochim Biophys Acta. 1975 Feb 10;378(3):383-93
references.pmid: 1115787
references.citation: Biochem Biophys Res Commun. 1962 Jun 4;7:486-90
references.pmid: 14498283
references.citation: J Mol Biol. 1976 Oct 5;106(4):1067-75
references.pmid: 824454
references.citation: J Mol Biol. 1976 Sep 15;106(2):403-20
references.pmid: 978727
references.citation: J Biol Chem. 1975 May 25;250(10):3725-8
references.pmid: 1126933
references.citation: Nat New Biol. 1973 Jan 17;241(107):66-8
references.pmid: 4512402
references.citation: Proc Natl Acad Sci U S A. 1973 Dec;70(12):3641-5
references.pmid: 4519651
references.citation: FEBS Lett. 1973 Jun 1;32(2):219-22
references.pmid: 4733778
references.citation: Ann N Y Acad Sci. 1974 Nov 29;241(0):25-38
references.pmid: 4611305