Separation and infectivity of circular and linear forms of potato spindle tuber viroid.
Potato spindle tuber viroid can be separated into two fractions by polyacrylamide gelelectrophoresis in the presence of formamide and urea. One fraction contains predominantly circular molecules; the second fraction contains almost exclusively linear molecules. The purity of the fractions was estimated by electron microscopy of formaldehyde-denatured molecules. The length distributions of denatured circular and linear molecules were determined. Both circular and linear molecules were found to be infectious.
D004591:Electrophoresis, Polyacrylamide Gel; D008854:Microscopy, Electron; D010941:Plant Tumors / Q000382:microbiology*; D010942:Plant Viruses / Q000502:physiology* / Q000648:ultrastructure
Virology. 1977 May 1;78(1):99-107
860413
Virology. 1977 Feb;76(2):477-84
841846
Proc Natl Acad Sci U S A. 1976 Nov;73(11):3852-6
1069269
Biochemistry. 1967 Jun;6(6):1818-27
6035921
Biochemistry. 1974 Dec 17;13(26):5367-73
4611483
Anal Biochem. 1974 Jun;59(2):599-609
4134777
Annu Rev Microbiol. 1974;28(0):23-39
4215365
Biochemistry. 1976 Apr 20;15(8):1663-7
178352
Proc Natl Acad Sci U S A. 1975 Jan;72(1):83-7
164030
Virology. 1973 Sep;55(1):70-80
4728831
Virology. 1973 Jun;53(2):359-65
4712388
Virology. 1972 Nov;50(2):606-9
4636118
Anal Biochem. 1976 Jan;70(1):75-85
1259158