Biochemical and morphological studies of rat submandibular gland: III. effects of testosterone treatment on proteins of granule-rickch fraction.
Mature adult male rats and castrated and testosterone-treated castrated adult rats were injected with pilocarpine HCl and 3-H-lysine and sacrificed sequentially over an eight-hour time period. Following homogenization and differential centrifugation, three subcellular fractions from each group of animals were analyzed by gel electrophoresis and liquid scintillation. Two proteins in the granule-rich fraction appeared in larger amounts on the densitometric scans and appeared to represent a larger proportion of newly synthesized proteins than the other proteins.
D000818:Animals; D002369:Castration; D003594:Cytoplasmic Granules / Q000201:enzymology / Q000378:metabolism*; D004950:Esterases / Q000378:metabolism; D005260:Female; D008297:Male; D008928:Mitochondria / Q000201:enzymology / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism; D051381:Rats; D012471:Salivary Proteins and Peptides / Q000032:analysis / Q000096:biosynthesis*; D013363:Submandibular Gland / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism / Q000648:ultrastructure; D013739:Testosterone / Q000494:pharmacology*
D012471:Salivary Proteins and Peptides; D013739:Testosterone; D004950:Esterases; D010447:Peptide Hydrolases