title: A new method for sequencing DNA.
abstract: DNA can be sequenced by a chemical procedure that breaks a terminally labeled DNA molecule partially at each repetition of a base. The lengths of the labeled fragments then identify the positions of that base. We describe reactions that cleave DNA preferentially at guanines, at adenines, at cytosines and thymines equally, and at cytosines alone. When the products of these four reactions are resolved by size, by electrophoresis on a polyacrylamide gel, the DNA sequence can be read from the pattern of radioactive bands. The technique will permit sequencing of at least 100 bases from the point of labeling.
mesh_terms: D000225:Adenine / Q000032:analysis; D001483:Base Sequence; D001669:Biochemical Phenomena; D001671:Biochemistry; D003596:Cytosine / Q000032:analysis; D004247:DNA; D004262:DNA Restriction Enzymes; D006147:Guanine / Q000032:analysis; D006834:Hydrazines; D008722:Methods; D009693:Nucleic Acid Hybridization; D013941:Thymine / Q000032:analysis
chemical_list: D006834:Hydrazines; D006147:Guanine; D003596:Cytosine; D004247:DNA; D004262:DNA Restriction Enzymes; D000225:Adenine; D013941:Thymine
references.citation: Biochim Biophys Acta. 1964 Nov 15;91:462-76
references.pmid: 14254018
references.citation: Biochim Biophys Acta. 1964 Sep 11;91:59-66
references.pmid: 14227279
references.citation: Biochem J. 1963 Oct;89:127-38
references.pmid: 14097355
references.citation: Biochemistry. 1973 Dec 4;12(25):5050-5
references.pmid: 4366306
references.citation: Biochemistry. 1975 Mar 25;14(6):1225-9
references.pmid: 164211
references.citation: Virology. 1972 Jul;49(1):342-4
references.pmid: 5039027
references.citation: Biochim Biophys Acta. 1969 Feb 18;174(2):591-603
references.pmid: 4888872
references.citation: Nucleic Acids Res. 1976 Apr;3(4):863-77
references.pmid: 775445
references.citation: J Mol Biol. 1976 Mar 25;102(1):157-65
references.pmid: 1271462
references.citation: J Chem Soc Perkin 1. 1967;16:1528-33
references.pmid: 5624304
references.citation: Biochem J. 1964 Jan;90(1):147-9
references.pmid: 5832284