New method for localizing proteins in periodic structures: Fab fragment labeling combined with image processing of electron micrographs.
Fab fragments prepared from antisera directed against purified bacteriophage T4 structural proteins and head-related structures were used to label proteins on the surface of T-even giant phage capsids. Optically filtered electron micrographs of the Fab-labeled capsids reveal both the location of specific proteins within the capsomeres and differing conformational states of the protein subunits. We describe parameters affecting the utility of this technique for the study of molecular organization and protein conformation in periodic biological structures.
D000914:Antibodies, Viral; D000937:Antigen-Antibody Reactions; D003090:Coliphages / Q000648:ultrastructure*; D007140:Immunoglobulin Fab Fragments; D008854:Microscopy, Electron / Q000379:methods; D011487:Protein Conformation; D014764:Viral Proteins / Q000276:immunology
D000914:Antibodies, Viral; D007140:Immunoglobulin Fab Fragments; D014764:Viral Proteins
Biochem J. 1959 Sep;73:119-26
14434282
J Ultrastruct Res. 1977 Apr;59(1):76-86
66324
J Mol Biol. 1977 Mar 15;110(4):687-98
859178
J Mol Biol. 1977 Feb 5;109(4):515-37
845938
J Supramol Struct. 1976;5(4):475-95
1027922
J Mol Biol. 1976 Oct 15;107(1):55-69
1003460
J Mol Biol. 1976 Sep 5;106(1):187-221
972397
J Mol Biol. 1973 Jan;73(1):37-53
4120454
Virology. 1973 Jul;54(1):245-61
4123461
J Virol. 1973 Aug;12(2):374-85
4127051
J Mol Biol. 1975 Mar 5;92(3):395-412
238040
J Supramol Struct. 1974;2(2-4):239-53
4437177
J Mol Biol. 1972 Apr 14;65(3):469-88
5023668
Annu Rev Biochem. 1975;44:639-67
806253
Annu Rev Biochem. 1975;44:161-82
1094909
J Mol Biol. 1975 Dec 25;99(4):631-44
765480
Proc Natl Acad Sci U S A. 1975 Dec;72(12):4820-4
1108004
J Mol Biol. 1976 May 25;103(3):469-98
781276
J Mol Biol. 1976 Apr 5;102(2):325-32
1271466
J Mol Biol. 1976 Feb 15;101(1):39-56
1255721
J Mol Biol. 1975 Oct 5;97(4):655-60
1185785
J Mol Biol. 1970 Jul 28;51(2):411-21
5530395
J Mol Biol. 1970 Jun 14;50(2):279-95
5476917