Calcium translocation by Golgi and lateral-basal membrane vesicles from rat intestine: decrease in vitamin D-deficient rats.
Intestinal Ca2+ transport was studied in membrane vesicles isolated from microvillus, Golgi, and lateral-basal membrane preparations. Ca2+ uptake by these vesicles was measured by determination of 45Ca2+ associated with these membranes after collection by micropore filtration. Golgi membranes showed the highest initial rate and equilibration level of Ca2+ uptake. Approximately 90% of this Ca2+ uptake was into an osmotically responsive space, suggesting that what was measured was predominantly Ca2+ translocation. Vitamin D-deficient rats showed a markedly diminished rate of uptake and level of equilibration. These data indicate that a Ca2+-translocating process was associated with Golgi membranes to a greater extent than with surface membranes and that this process was markedly decreased in vitamin D-deficient rats. The results suggest that the Golgi apparatus participates in intestinal Ca2+ absorption.
D000818:Animals; D001693:Biological Transport, Active; D002118:Calcium / Q000378:metabolism*; D002458:Cell Fractionation; D006056:Golgi Apparatus / Q000378:metabolism* / Q000648:ultrastructure; D007413:Intestinal Mucosa / Q000378:metabolism*; D007421:Intestine, Small / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D008566:Membranes / Q000378:metabolism / Q000648:ultrastructure; D008854:Microscopy, Electron; D008871:Microvilli / Q000378:metabolism / Q000648:ultrastructure; D009994:Osmolar Concentration; D051381:Rats; D014808:Vitamin D Deficiency / Q000378:metabolism*
D002118:Calcium
J Membr Biol. 1976 Sep 17;28(4):309-33
136516
Annu Rev Biochem. 1976;45:631-66
183601
Biochem Biophys Res Commun. 1975 Jul 22;65(2):545-51
238533
Anal Biochem. 1975 Jul;67(1):44-54
807125
Biochem J. 1975 Jan;146(1):213-21
807201
Nature. 1976 Sep 9;263(5573):161-3
967255
J Biol Chem. 1975 Apr 10;250(7):2411-9
1079026
Science. 1975 Aug 1;189(4200):347-58
1096303
J Cell Biol. 1975 Jan;64(1):54-74
1109237
Biochem Biophys Res Commun. 1969 Jun 27;35(6):819-23
4239916
Physiol Rev. 1973 Apr;53(2):327-72
4267263
J Clin Invest. 1973 Nov;52(11):2765-73
4270644
Am J Physiol. 1966 Nov;211(5):1131-6
4288601
J Biol Chem. 1968 Jul 25;243(14):3978-86
4298516
Biochim Biophys Acta. 1969 Apr;173(3):456-68
4305976
J Histochem Cytochem. 1970 Feb;18(2):107-15
4313404
Biochemistry. 1970 Apr 14;9(8):1658-64
4314699
Pharmacol Rev. 1970 Sep;22(3):389-428
4322219
J Biol Chem. 1970 Sep 10;245(17):4432-42
4322438
Lab Invest. 1971 Jul;25(1):42-8
4326524
J Cell Biol. 1972 Jan;52(1):15-40
4331298
Fed Proc. 1973 Sep;32(9):1944-50
4353596
Science. 1972 Feb 11;175(4022):596-9
4550618
J Biol Chem. 1973 Jan 10;248(1):25-32
4692832
J Biol Chem. 1973 Apr 10;248(7):2536-41
4698230
J Physiol. 1973 Nov;235(1):75-102
4778143
J Clin Invest. 1974 Sep;54(3):710-7
4852407
J Cell Biol. 1969 Nov;43(2):289-311
5344149
J Cell Biol. 1970 Jun;45(3):635-40
5459946
J Cell Biol. 1970 Jul;46(1):52-63
5460463
Calcif Tissue Res. 1970;5(4):305-16
5469610
Lab Invest. 1968 Nov;19(5):516-25
5698733
Anal Biochem. 1966 Mar;14(3):376-92
5944942
Biochem J. 1955 Aug;60(4):604-17
13249955
J Biol Chem. 1951 Apr;189(2):665-70
14832284
J Biol Chem. 1951 Nov;193(1):265-75
14907713
Science. 1966 May 6;152(3723):791-3
17797460