title: Distinctions between the two-state and sequential models for cooperative ligand binding.
abstract: The two-state and sequential models for positive cooperativity in ligand binding can produce significantly different theoretical binding curves when presented in a Scatchard plot. The conditions that produce the greatest differences have been examined. The theoretical differences have been used to select the two-state model as the best model for describing the binding of acetylcholine to acetylcholine receptors that have been solubilized by Triton X-100 and sodium cholate.
mesh_terms: D000109:Acetylcholine / Q000378:metabolism*; D000495:Allosteric Site; D000818:Animals; D004557:Electric Organ; D005399:Fishes; D007700:Kinetics; D008024:Ligands; D008954:Models, Biological; D011950:Receptors, Cholinergic / Q000378:metabolism*
chemical_list: D008024:Ligands; D011950:Receptors, Cholinergic; D000109:Acetylcholine
references.citation: Proc Natl Acad Sci U S A. 1935 Apr;21(4):186-91
references.pmid: 16587956
references.citation: J Mol Biol. 1965 May;12:88-118
references.pmid: 14343300
references.citation: Annu Rev Biophys Bioeng. 1974;3(0):1-33
references.pmid: 4371650
references.citation: Biochemistry. 1970 Aug 18;9(17):3325-36
references.pmid: 4330854
references.citation: Biochemistry. 1966 Jan;5(1):365-85
references.pmid: 5938952
references.citation: Annu Rev Biochem. 1975;44:209-32
references.pmid: 237460
references.citation: Biochemistry. 1974 Nov 19;13(24):4998-5002
references.pmid: 4433533
references.citation: Biochemistry. 1976 Jan 27;15(2):277-82
references.pmid: 764863
references.citation: Arch Biochem Biophys. 1975 Aug;169(2):458-63
references.pmid: 1180559
references.citation: Biochemistry. 1975 Dec 16;14(25):5428-37
references.pmid: 55