Chemistry of the sulfate groups in a sulfated glycoprotein from rabbit submandibular gland.
A 35S-labeled sulfated glycoprotein was isolated from rabbit submandibular glands. Acid stability studies on the 35sulfate groupings present in the intact glycoprotein gave a half-life of 45 min. Partial acid hydrolysis of the 35S-labeled glycoprotein in 0.1 M HC1 for 90 min at 100 degrees C liberated a radioactive fraction which was free from peptide and fractionated in the monosaccharide range of a Sephadex G-15 column. Examination of this fraction by paper chromatography revealed the presence of a major component having the characteristics of N-acetylglucosamine 6-0-sulfate and a minor component having the properties of N-acetylgalactosamine 6-0-sulfate. The presence of ester sulfate groups in the intact glycoprotein was confirmed by infrared spectroscopy.
D000818:Animals; D002854:Chromatography, Paper; D006023:Glycoproteins / Q000032:analysis* / Q000302:isolation & purification; D006207:Half-Life; D006868:Hydrolysis; D011817:Rabbits; D013057:Spectrum Analysis; D013363:Submandibular Gland / Q000032:analysis*; D013431:Sulfates / Q000032:analysis* / Q000302:isolation & purification; D013462:Sulfur Radioisotopes
D006023:Glycoproteins; D013431:Sulfates; D013462:Sulfur Radioisotopes