title: Distribution of rat liver albumin mRNA membrane-bound and free in polyribosomes as determined by molecular hybridization.
abstract: Recently, we purified rat liver albumin mRNA and prepared albumin [(3)H]cDNA. Using albumin [(3)H]cDNA in molecular hybridization experiments, we have now determined the distribution of albumin mRNA sequences in membrane-bound and free liver polyribosomes prepared by techniques in which there is high recovery of polyribosomes without evidence of degradation. By using molecular hybridization to measure specific mRNA sequence content, numerous problems could be avoided in interpretation of results as obtained by cell-free protein synthesis or immunological methods. Under these conditions, 98% of albumin mRNA sequences in polyribosomes are found in the membrane-bound fraction.
mesh_terms: D000418:Albumins / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D003593:Cytoplasm / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism; D008099:Liver / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D011061:Poly A / Q000378:metabolism; D011132:Polyribosomes / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats
chemical_list: D000418:Albumins; D012333:RNA, Messenger; D011061:Poly A
references.citation: Biochemistry. 1972 May 23;11(11):2061-6
references.pmid: 5027616
references.citation: Ser Haematol. 1971;4(3):37-69
references.pmid: 5149467
references.citation: Biochem Biophys Res Commun. 1972 Nov 1;49(3):713-9
references.pmid: 4638750
references.citation: J Biochem. 1973 May;73(5):1083-8
references.pmid: 4721773
references.citation: Proc Natl Acad Sci U S A. 1977 Apr;74(4):1358-62
references.pmid: 266178
references.citation: Biochemistry. 1976 Dec 14;15(25):5501-6
references.pmid: 999824
references.citation: Proc Natl Acad Sci U S A. 1977 Feb;74(2):716-20
references.pmid: 403522
references.citation: J Biol Chem. 1962 Apr;237:1186-9
references.pmid: 14485749
references.citation: Proc Natl Acad Sci U S A. 1971 May;68(5):1075-8
references.pmid: 4325000
references.citation: Biochemistry. 1976 Jan 13;15(1):15-9
references.pmid: 1247503
references.citation: Ann N Y Acad Sci. 1974 Nov 29;241(0):280-9
references.pmid: 4139921
references.citation: J Biol Chem. 1969 Aug 25;244(16):4308-15
references.pmid: 5806579
references.citation: Proc Natl Acad Sci U S A. 1968 Feb;59(2):582-9
references.pmid: 5238986
references.citation: Proc Natl Acad Sci U S A. 1974 Jun;71(6):2428-31
references.pmid: 4526305
references.citation: Nucleic Acids Res. 1976 Jun;3(6):1427-36
references.pmid: 986643
references.citation: J Clin Invest. 1973 Jul;52(7):1735-45
references.pmid: 4718962
references.citation: Science. 1969 May 2;164(3879):584-5
references.pmid: 5781696
references.citation: J Biol Chem. 1974 Oct 25;249(20):6517-26
references.pmid: 4421725
references.citation: J Biol Chem. 1975 Jul 25;250(14):5688-95
references.pmid: 167021
references.citation: J Biol Chem. 1977 Feb 25;252(4):1272-8
references.pmid: 14149
references.citation: J Biol Chem. 1974 Jan 10;249(1):89-93
references.pmid: 4809633
references.citation: Nat New Biol. 1973 Jan 31;241(109):150-2
references.pmid: 4512619
references.citation: J Mol Biol. 1972 Jan 28;63(2):247-64
references.pmid: 4634507
references.citation: Biochim Biophys Acta. 1975 Oct 15;407(3):325-37
references.pmid: 810623
references.citation: Proc Natl Acad Sci U S A. 1974 Jul;71(7):2863-6
references.pmid: 4528029
references.citation: Proc Natl Acad Sci U S A. 1966 May;55(5):1283-8
references.pmid: 5225526
references.citation: Eur J Biochem. 1970 Feb;12(2):392-8
references.pmid: 5459577
references.citation: Cell. 1977 Feb;10(2):253-63
references.pmid: 837448
references.citation: Proc Natl Acad Sci U S A. 1973 Aug;70(8):2330-4
references.pmid: 4525168
references.citation: Proc Natl Acad Sci U S A. 1972 Jun;69(6):1565-8
references.pmid: 4504369
references.citation: Biochim Biophys Acta. 1970 Sep 17;217(1):148-58
references.pmid: 5505328
references.citation: J Cell Biol. 1975 Dec;67(3):835-51
references.pmid: 811671
references.citation: Biochem Biophys Res Commun. 1968 Oct 10;33(1):55-60
references.pmid: 5696506
references.citation: J Biol Chem. 1974 Jan 10;249(1):81-8
references.pmid: 4203523
references.citation: J Cell Biol. 1977 Mar;72(3):726-43
references.pmid: 838773
references.citation: J Cell Biol. 1977 Jul;74(1):43-57
references.pmid: 194907
references.citation: Proc Natl Acad Sci U S A. 1977 Oct;74(10):4346-50
references.pmid: 270676
references.citation: Biochim Biophys Acta. 1977 Jan 20;474(2):234-44
references.pmid: 851473
references.citation: J Cell Biol. 1973 Oct;59(1):28-44
references.pmid: 4201708
references.citation: Biochem J. 1971 Feb;121(4):683-94
references.pmid: 5114978
references.citation: J Biol Chem. 1974 Nov 10;249(21):6779-87
references.pmid: 4420185
references.citation: J Biophys Biochem Cytol. 1960 Jul;7:619-30
references.pmid: 14446510
references.citation: Biochem J. 1969 Apr;112(2):139-47
references.pmid: 4389820
references.citation: Biochim Biophys Acta. 1972 Nov 16;287(1):113-23
references.pmid: 4347125
references.citation: Biochem Biophys Res Commun. 1971 Jan 8;42(1):125-31
references.pmid: 5546347
references.citation: Biochemistry. 1976 Apr 20;15(8):1704-12
references.pmid: 1268192