title: Independent expression of the adrenergic phenotype by neural crest cells in vitro.
abstract: Neural crest cells obtained from Japanese quail and grown in vitro without other embryonic tissues differentiate into adrenergic cells. These cells show intense catecholamine-specific histochemical fluorescence, and some have long, varicose neuronal processes. Ultrastructural examination shows two populations of cells, one with small (about 90 nm) dense-core vesicles resembling principal sympathetic neurons and the other with larger (about 150 nm) dense-core granules resembling chromaffin or small intensely fluorescent cells. Neuronal cells without adrenergic characteristics are also present. These results are compatible with the hypothesis that a population of cells determined along neuronal lines exists in the neural crest prior to migration.
mesh_terms: D000818:Animals; D002395:Catecholamines / Q000378:metabolism*; D002454:Cell Differentiation; D002838:Chromaffin System / Q000196:embryology* / Q000648:ultrastructure; D003370:Coturnix; D004477:Ectogenesis; D005725:Ganglia, Autonomic / Q000196:embryology* / Q000648:ultrastructure; D008544:Melanocytes / Q000166:cytology; D009420:Nervous System / Q000196:embryology*; D010641:Phenotype
chemical_list: D002395:Catecholamines
references.citation: Proc Natl Acad Sci U S A. 1976 Nov;73(11):4225-9
references.pmid: 186792
references.citation: Proc Natl Acad Sci U S A. 1976 Nov;73(11):4220-4
references.pmid: 1069311
references.citation: Brain Res. 1972 Nov 13;46:159-75
references.pmid: 4117873
references.citation: Dev Biol. 1974 Nov;41(1):162-84
references.pmid: 4140118
references.citation: J Neurochem. 1974 Feb;22(2):301-6
references.pmid: 4151460
references.citation: Brain Res. 1971 Nov;34(2):229-40
references.pmid: 4401209
references.citation: Pharmacol Rev. 1972 Jun;24(2):255-67
references.pmid: 4404612
references.citation: J Neurocytol. 1974 Aug;3(3):363-84
references.pmid: 4373543
references.citation: J Cell Biol. 1973 Apr;57(1):88-108
references.pmid: 4347980
references.citation: Nature. 1976 Jul 22;262(5566):308-10
references.pmid: 183125
references.citation: Proc Natl Acad Sci U S A. 1974 Sep;71(9):3607-10
references.pmid: 4279409
references.citation: J Biol Chem. 1966 May 25;241(10):2301-5
references.pmid: 4287854
references.citation: Z Zellforsch Mikrosk Anat. 1972;135(3):287-314
references.pmid: 4566367
references.citation: Brain Res. 1976 Feb 6;102(2):301-12
references.pmid: 2367
references.citation: J Cell Biol. 1972 May;53(2):348-64
references.pmid: 4554365
references.citation: Histochem J. 1972 Jan;4(1):49-58
references.pmid: 4553247
references.citation: Exp Neurol. 1974 Oct;45(1):94-103
references.pmid: 4472205
references.citation: J Cell Biol. 1976 Feb;68(2):240-63
references.pmid: 173724
references.citation: Dev Biol. 1973 Sep;34(1):16-38
references.pmid: 4787602
references.citation: J Anat. 1973 May;115(Pt 1):119-31
references.pmid: 4728263
references.citation: J Exp Zool. 1972 Feb;179(2):167-82
references.pmid: 5059187
references.citation: Anat Embryol (Berl). 1975 May 16;146(3):225-43
references.pmid: 1096675
references.citation: Brain Res. 1976 Sep 24;114(3):391-414
references.pmid: 953763
references.citation: Cell Tissue Res. 1976 Jan 28;165(4):477-508
references.pmid: 1260841
references.citation: Dev Biol. 1976 Mar;49(1):66-79
references.pmid: 1254098
references.citation: J Neurocytol. 1975 Dec;4(6):633-46
references.pmid: 1238511
references.citation: Dev Biol. 1975 Oct;46(2):262-80
references.pmid: 1183721
references.citation: Dev Biol. 1975 Jan;42(1):106-30
references.pmid: 1112437
references.citation: Dev Biol. 1971 Dec;26(4):578-605
references.pmid: 5134613
references.citation: J Anat. 1969 Sep;105(Pt 2):255-82
references.pmid: 5802933
references.citation: Acta Neuroveg (Wien). 1967;30(1):427-44
references.pmid: 6080700