title: Amino acid sequence of a myosin fragment that contains SH-1, SH-2, and Ntau-methylhistidine.
abstract: A peptide having 92 amino acid residues and a calculated molecular weight of 10,478 was isolated from a cyanogen bromide digest of rabbit skeletal muscle myosin. It contained both proline and Ntau-methylhistidine, indicating that it arose from the portion of the heavy chain that folds to form most of the globular head of the myosin molecule. The amino acid sequence of the peptide included the two sulfhydryl groups whose alkylation modifies myosin's catalytic properties: SH-2 at position 11 in the peptide, and SH-1 at position 21. This proximity in the sequence means that SH-1 and SH-2 must be relatively close together in myosin, and several lines of evidence suggest that this region is near the catalytic or actin binding site(s) of myosin.
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000478:Alkylation; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites / Q000187:drug effects; D006639:Histidine / Q000031:analogs & derivatives*; D066298:In Vitro Techniques; D008762:Methylhistidines / Q000032:analysis*; D009218:Myosins; D011817:Rabbits; D013438:Sulfhydryl Compounds / Q000032:analysis*
chemical_list: D008762:Methylhistidines; D013438:Sulfhydryl Compounds; D006639:Histidine; D000255:Adenosine Triphosphate; D009218:Myosins
references.citation: J Biochem. 1965 Mar;57:460-1
references.pmid: 14330734
references.citation: Biochim Biophys Acta. 1965 May 4;100:459-70
references.pmid: 14347944
references.citation: Biochem Z. 1964 Jun 16;339:539-42
references.pmid: 14254926
references.citation: J Biochem. 1964 May;55:576-7
references.pmid: 14185967
references.citation: Biochem Z. 1963;337:109-14
references.pmid: 13950638
references.citation: Biochem J. 1962 Dec;85:431-9
references.pmid: 13936493
references.citation: J Biol Chem. 1962 Sep;237:2769-72
references.pmid: 13910381
references.citation: J Biol Chem. 1956 Feb;218(2):653-9
references.pmid: 13295220
references.citation: Science. 1977 Mar 4;195(4281):884-6
references.pmid: 190679
references.citation: J Biochem. 1976 Jul;80(1):101-10
references.pmid: 9375
references.citation: J Biol Chem. 1969 Feb 25;244(4):648-57
references.pmid: 4305880
references.citation: Anal Biochem. 1971 Feb;39(2):462-77
references.pmid: 4101989
references.citation: Biochemistry. 1974 Jan 15;13(2):222-45
references.pmid: 4358940
references.citation: CRC Crit Rev Biochem. 1974 Jan;2(1):1-65
references.pmid: 4273099
references.citation: Biochemistry. 1970 Mar 17;9(6):1365-74
references.pmid: 5418372
references.citation: Arch Biochem Biophys. 1973 Aug;157(2):588-96
references.pmid: 4354323
references.citation: Biochemistry. 1974 May 7;13(10):2014-22
references.pmid: 4275027
references.citation: J Biochem. 1974 Mar;75(3):447-53
references.pmid: 4276126
references.citation: Biochemistry. 1974 Sep 10;13(19):3837-40
references.pmid: 4278279
references.citation: J Biochem. 1966 Jan;59(1):24-33
references.pmid: 4223102
references.citation: J Mol Biol. 1973 Nov 25;81(1):17-31
references.pmid: 4588573
references.citation: Nature. 1976 Feb 26;259(5545):699-700
references.pmid: 2877
references.citation: Annu Rev Biochem. 1976;45:427-65
references.pmid: 134667
references.citation: Eur J Biochem. 1974 May 15;44(2):317-34
references.pmid: 4838672
references.citation: FEBS Lett. 1973 Nov 1;36(3):285-8
references.pmid: 4797036
references.citation: J Biol Chem. 1972 Feb 10;247(3):745-53
references.pmid: 5058224
references.citation: Nature. 1968 Feb 3;217(5127):452-3
references.pmid: 4966706
references.citation: J Mol Biol. 1975 May 25;94(3):311-26
references.pmid: 1177301
references.citation: FEBS Lett. 1975 Feb 1;50(2):159-62
references.pmid: 1167528
references.citation: Biochemistry. 1971 Dec 21;10(26):4912-21
references.pmid: 5134536
references.citation: Eur J Biochem. 1971 May 11;20(1):89-102
references.pmid: 5578618
references.citation: Nature. 1969 Jul 19;223(5203):300-2
references.pmid: 5792725
references.citation: Biochem J. 1968 Apr;107(4):531-48
references.pmid: 5660634
references.citation: Biochem Biophys Res Commun. 1967 Jan 23;26(2):168-74
references.pmid: 6067661
references.citation: J Mol Biol. 1967 Feb 14;23(3):287-304
references.pmid: 6032180