Kinetics of biphasic reconstitution of tobacco mosaic virus in vitro.
The kinetics of the in vitro reconstitution of tobacco mosaic virus from its RNA and protein were studied by measuring the increase in turbidity, the development of ribonuclease-resistant infectivity, the emcapsidation of the terminal ends of the RNA, and the growth of rod length. The results showed that the reconstitution reaction consists of two processes in which the direction, timing, and rate of assembly are different. Rapid elongation of particles toward the 5' end of the RNA proceeds in the first 5-7 min to give intermediate particles of 260 nm in length in which only the 5' terminus of the RNA is encapsidated. The subsequent process requires 30-50 min, is accompanied by a slow increase in turbidity, and gives rise to rods of the full length, 300 nm. The 3' terminus becomes RNase resistant by this process with concomitant development of ribonuclease-resistant infectivity, showing that the 3'-distal portion of the RNA is encapsidated in the direction of 5' to 3'. The rate of rod elongation by the second process is less than 1/10 of that by the first process.
D002213:Capsid / Q000378:metabolism; D066298:In Vitro Techniques; D007700:Kinetics; D011485:Protein Binding; D012367:RNA, Viral / Q000378:metabolism*; D014027:Tobacco Mosaic Virus / Q000254:growth & development / Q000648:ultrastructure*; D014764:Viral Proteins / Q000378:metabolism*
D012367:RNA, Viral; D014764:Viral Proteins
J Mol Biol. 1974 Jan 25;82(3):333-41
4817789
Proc Natl Acad Sci U S A. 1972 Oct;69(10):2950-3
4342969
Biochemistry. 1973 Nov 6;12(23):4574-81
4359368
Virology. 1977 Jan;76(1):429-32
13534
Virology. 1971 Jun;44(3):510-6
5137798
Virology. 1961 May;14:54-8
13701154
J Mol Biol. 1972 Dec 14;72(1):25-35
4648115
Virology. 1976 Sep;73(2):363-71
960569
Virology. 1966 Nov;30(3):341-7
5921640
FEBS Lett. 1975 Sep 1;57(1):31-4
170129
Proc Natl Acad Sci U S A. 1977 Feb;74(2):552-5
265520
Virology. 1977 May 15;78(2):407-14
867810
Nucleic Acids Res. 1975 Jul;2(7):1189-201
168558
Proc Natl Acad Sci U S A. 1977 May;74(5):1913-7
325560
Proc Natl Acad Sci U S A. 1977 Jan;74(1):149-53
264669
Virology. 1957 Aug;4(1):1-4
13456355
J Mol Biol. 1973 Aug 25;78(4):637-49
4762924
FEBS Lett. 1976 Dec 1;71(2):294-8
826409
Nature. 1977 Jan 20;265(5591):217-9
834264
Nature. 1976 Apr 29;260(5554):759-64
1264250
FEBS Lett. 1971 Aug 15;16(3):204-206
11945941
Nat New Biol. 1971 Jan 13;229(2):47-50
5276072