title: Transfer of mannose from mannosyl retinyl phosphate to protein.
abstract: Upon incubation of [14C]mannose-labeled mannosyl retinyl phosphate with a membrane fraction from rat liver, mannose was transferred to an endogenous acceptor precipitable withchloroform/methanol to the extent of about 7%. The reaction proceeded linearly with time for 120 min at a pH optimum of about 7.0. The acceptor thus labeled with mannose could be solubilized by sodium dodecyl sulfate/mercaptoethanol. More than half of this acceptor appeared in the void volume of a Sephadex G-100 column. When it was digested with Pronase, a substantial proportion of it appeared between the void and bed volumes of a Sephadex G-100 column, thus indicating that it was a glycopeptide. In high-voltage paper electrophoresis, this glycopeptide moved to the cathode at low pH and to the anode at highpH. When digested with highly purified jack bean alpha-mannosidase, the glycopeptide released almost 50% of its radioactivity as mannose. That this transfer of mannose to glycoprotein from mannosyl retinyl phosphate does not take place via dolichyl mannosyl phosphate was shown by the fact that it is Mn2+ and Mg2+ independent, it is not inhibited by the presence of a 10-fold molar excess of nonradioactive GDP-mannose, and neither 14C-labeled dolichyl mannosyl phosphate nor 14-C labeled lipid pyrophosphoryl oligosaccharide could be detected during the incubation.
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D004224:Diterpenes; D007700:Kinetics; D008099:Liver / Q000378:metabolism; D008358:Mannose / Q000031:analogs & derivatives / Q000378:metabolism*; D011103:Polyisoprenyl Phosphate Monosaccharides / Q000378:metabolism*; D011105:Polyisoprenyl Phosphate Sugars / Q000378:metabolism*; D051381:Rats; D014801:Vitamin A / Q000031:analogs & derivatives / Q000378:metabolism; D014802:Vitamin A Deficiency / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D004224:Diterpenes; D011103:Polyisoprenyl Phosphate Monosaccharides; D011105:Polyisoprenyl Phosphate Sugars; D014801:Vitamin A; C013101:mannosylretinylphosphate; D008358:Mannose
references.citation: J Biol Chem. 1951 Nov;193(1):265-75
references.pmid: 14907713
references.citation: J Nutr. 1976 Nov;106(11):1659-67
references.pmid: 62042
references.citation: Biochim Biophys Acta. 1972 Aug 11;270(4):529-36
references.pmid: 5054304
references.citation: J Biol Chem. 1972 Mar 25;247(6):1836-48
references.pmid: 4335100
references.citation: Vitam Horm. 1974;32:181-214
references.pmid: 4617399
references.citation: J Biol Chem. 1976 Aug 25;251(16):4986-95
references.pmid: 8451
references.citation: J Lipid Res. 1975 May;16(3):235-43
references.pmid: 236352
references.citation: Science. 1975 Jun 6;188(4192):986-91
references.pmid: 167438
references.citation: Biochem Biophys Res Commun. 1974 Sep 9;60(1):355-63
references.pmid: 4421270
references.citation: J Nutr. 1974 Jun;104(6):710-8
references.pmid: 4830275
references.citation: J Biol Chem. 1973 Jan 25;248(2):641-8
references.pmid: 4684696
references.citation: Biochim Biophys Acta. 1973 Feb 28;297(2):441-51
references.pmid: 4705475
references.citation: Biochem J. 1968 Dec;110(4):663-70
references.pmid: 4973951
references.citation: Proc Natl Acad Sci U S A. 1971 Nov;68(11):2857-60
references.pmid: 5288263
references.citation: Proc Natl Acad Sci U S A. 1970 May;66(1):153-9
references.pmid: 5273893
references.citation: Proc Natl Acad Sci U S A. 1965 Jul;54(1):235-41
references.pmid: 5216357
references.citation: J Biol Chem. 1970 Sep 10;245(17):4551-8
references.pmid: 5498435
references.citation: J Biol Chem. 1965 Oct;240(10):3707-13
references.pmid: 5842047
references.citation: J Biol Chem. 1967 Dec 10;242(23):5474-80
references.pmid: 12325362