Fluorescence redistribution after photobleaching. A new multipoint analysis of membrane translational dynamics.
A theoretical formulation and experimental methodology are presented for a new multipoint analysis of membrane translational dynamics. The redistribution of fluorescent probe after a localized photobleaching pulse is monitored at several locations by a focused laser beam sequentially scanned through the bleached area. The spatial information so obtained provides a unique sensitivity to possible systematic flow and a direct internal calibration of the characteristic transport distance. These capabilities are demonstrated with experimental data on a reconstituted multibilayer system.
D002462:Cell Membrane / Q000502:physiology*; D007700:Kinetics; D008433:Mathematics; D008954:Models, Biological; D008968:Molecular Conformation; D010782:Photolysis; D013050:Spectrometry, Fluorescence / Q000295:instrumentation
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