title: Molecular properties of the nerve growth factor secreted by L cells.
abstract: The molecular size and stability of the nerve growth factor (NGF) secreted in culture by L cells have been studied by sedimentation and gel filtration chromatography. Results indicate that L cell NGF has a molecular weight of 160,000. It contains as part of its structure a protein component that is biologically, immunologically, and electrophoretically indistinguishable from the biologically active factor purified from mouse submandibular glands. However, unlike pure gland NGF, L cell NGF is highly stable in solution, and this finding indicates that L cell NGF is a form of the factor different from that previously described.
mesh_terms: D000818:Animals; D001681:Biological Assay; D002642:Chick Embryo; D004591:Electrophoresis, Polyacrylamide Gel; D007739:L Cells / Q000378:metabolism*; D009414:Nerve Growth Factors / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D009414:Nerve Growth Factors
references.citation: J Cell Biol. 1977 Mar;72(3):769-73
references.pmid: 838775
references.citation: Proc Natl Acad Sci U S A. 1974 Apr;71(4):1554-8
references.pmid: 4524658
references.citation: Annu Rev Biochem. 1976;45:531-58
references.pmid: 786157
references.citation: Biochemistry. 1973 Jan 2;12(1):100-15
references.pmid: 4566923
references.citation: J Biol Chem. 1976 Jan 25;251(2):459-64
references.pmid: 1394
references.citation: Proc Natl Acad Sci U S A. 1969 Oct;64(2):787-94
references.pmid: 4982360
references.citation: Physiol Rev. 1968 Jul;48(3):534-69
references.pmid: 4875350
references.citation: J Exp Zool. 1965 Nov;160(2):183-8
references.pmid: 5327556
references.citation: Biochem Pharmacol. 1976 Jul 1;25(13):1445-9
references.pmid: 782456
references.citation: Proc Natl Acad Sci U S A. 1975 May;72(5):1895-8
references.pmid: 1057179
references.citation: J Neurochem. 1975 Dec;25(6):843-51
references.pmid: 1206401
references.citation: Science. 1975 Jan 31;187(4174):361-2
references.pmid: 1167427
references.citation: Proc Natl Acad Sci U S A. 1967 Jun;57(6):1782-9
references.pmid: 5231412
references.citation: Biochemistry. 1971 Feb 2;10(3):463-9
references.pmid: 5543976
references.citation: Arch Biochem Biophys. 1969 Mar;130(1):337-46
references.pmid: 5778650
references.citation: Biochemistry. 1968 Apr;7(4):1296-303
references.pmid: 5677819
references.citation: Biochemistry. 1967 Jul;6(7):2202-9
references.pmid: 6049455