title: The isolation and partial characterization of the major bronchial glycoproteins.
abstract: Partial characterization of glycoprotein obtained from mucous secretion of the bronchi and stomach has been attempted. The isolated glycoproteins and the glycoproteins from gastric aspirates showed similar carbohydrate and amino-acid composition. They consist of a protein core to which are attached carbohydrate side chains of galactose, N-acetylglucosamine and N-acetylgalactosamine in the ratio of 4 : 3 : 1. Superimposed on this structure were additional sugar residues, the blood group determinants. The carbohydrate side chains are linked by an alkali-labile O-glycosidic linkage to the threonine and serine residues of the protein core, with N-acetylgalactosamine forming the link.
mesh_terms: D000116:Acetylgalactosamine / Q000032:analysis; D000117:Acetylglucosamine / Q000032:analysis; D000596:Amino Acids / Q000032:analysis*; D001789:Blood Group Antigens; D001980:Bronchi / Q000378:metabolism*; D002241:Carbohydrates / Q000032:analysis*; D005643:Fucose / Q000032:analysis; D005690:Galactose / Q000032:analysis; D005753:Gastric Mucosa / Q000378:metabolism; D006023:Glycoproteins / Q000032:analysis / Q000302:isolation & purification*; D006801:Humans; D008968:Molecular Conformation; D009092:Mucous Membrane / Q000378:metabolism; D012694:Serine / Q000032:analysis; D013183:Sputum / Q000032:analysis; D013912:Threonine / Q000032:analysis
chemical_list: D000596:Amino Acids; D001789:Blood Group Antigens; D002241:Carbohydrates; D006023:Glycoproteins; D005643:Fucose; D013912:Threonine; D012694:Serine; D000116:Acetylgalactosamine; D000117:Acetylglucosamine; D005690:Galactose