Angiotensin stimulation of bovine adrenocortical cell growth.
Factors controlling proliferation of adrenocortical cells have been studied in monolayer cultures of bovine adrenocortical cells. Angiotensin II stimulated cell proliferation and [3H]thymidine incorporation into DNA with a half-maximal effective concentration of 0.96 +/- 0.27 nM. Similar sensitivity to angiotensin III with reduced sensitivity to angiotensin I and tetradecapeptide renin substrate was observed. Although sensitivity to angiotensin II was equivalent to that for fibroblast growth factor (1.5 nM half-maximal effective concentration), maximal effects of angiotensin were less than for fibroblast growth factor and serum. High concentrations of insulin (1-10 micrometer) also stimulated [3H]thymidine incorporation into DNA and cell proliferation. [Sar1,Ile5,Ile8]Angiotensin II, a competitive antagonist of angiotensin II, blocked angiotensin II stimulation of DNA synthesis but did not affect fibroblast growth factor and insulin stimulation of DNA synthesis. Corticotropin (ACTH) blocked the stimulatory effects of both angiotensin II and fibroblast growth factor. The dose-response curves for angiotensin II stimulation of steroidogenesis were similar to those for stimulation of [3H]thymidine incorporation into DNA. Among the seven cell types examined, only adrenocortical cells responded to angiotension II with stimulation of DNA synthesis.
D000302:Adrenal Cortex / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D000305:Adrenal Cortex Hormones / Q000096:biosynthesis; D000809:Angiotensins / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D006133:Growth Substances / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology
D000305:Adrenal Cortex Hormones; D000809:Angiotensins; D006133:Growth Substances; D007328:Insulin; D004247:DNA
J Exp Med. 1956 Feb 1;103(2):273-83
13286432
Endocrinology. 1977 Apr;100(4):1108-20
300054
Endocrinology. 1977 Apr;100(4):1080-9
189990
J Clin Invest. 1977 Aug;60(2):342-52
194925
Mol Cell Endocrinol. 1977 Apr;7(2):151-65
193747
Am J Med. 1976 Nov;61(5):758-67
185901
Clin Exp Pharmacol Physiol. 1976 Mar-Apr;3(2):103-26
788973
Proc Natl Acad Sci U S A. 1976 Nov;73(11):4120-4
1069301
J Cell Physiol. 1968 Oct;72(2):109-14
4176502
J Biol Chem. 1974 Feb 10;249(3):825-34
4359769
Nat New Biol. 1973 May 16;243(124):85-7
4349963
Proc Natl Acad Sci U S A. 1974 Feb;71(2):341-4
4360940
J Med Chem. 1972 Aug;15(8):792-5
4339769
Proc Natl Acad Sci U S A. 1971 Dec;68(12):3206-10
4332015
Recent Prog Horm Res. 1970;26:589-622
4319353
Annu Rev Biochem. 1976;45:531-58
786157
Proc Natl Acad Sci U S A. 1976 May;73(5):1612-6
179091
Proc Natl Acad Sci U S A. 1975 Jan;72(1):113-7
164010
Endocrinology. 1975 Feb;96(2):458-67
163187
Endocrinology. 1975 Dec;97(6):1577-86
173529
Methods Biochem Anal. 1966;14:63-78
5328507
Nature. 1976 Jan 15;259(5539):132-4
813153
Proc Natl Acad Sci U S A. 1976 May;73(5):1617-20
1064032
J Biol Chem. 1975 Apr 10;250(7):2515-20
1168187