Lack of subclass selection during density purification of human lymphocytes.
The percentage of acid alpha-naphthyl acetate esterase (ANAE) marker-carrying lymphocytes (T-lymphocytes) was determined directly from the blood, after density purification of lymphocytes over Ficoll-Isopaque and removal of monocytes by iron powder plus magnet. The effect of Ficoll-Isopaque centrifugation was insignificant; neither did the iron powder plus magnet procedure increase the relative proportion of the T cells in these preparations. We therefore conclude that in optimal conditions there is no subclass-specific enrichment of T or B lymphocytes during the purification procedure.
D001402:B-Lymphocytes; D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient; D006801:Humans; D007958:Leukocyte Count; D009000:Monocytes; D013601:T-Lymphocytes