[PHA- and PWM-stimulation in acute leukaemia (author's transl)].
Peripheral blood cells from patients with acute leukaemia were stimulated in RPMI-medium with Phytohaemagglutinin (PHA) and/or Pokeweed-Mitogen (PWM). Samples of the cultures were taken at 3, 5 and 7 day intervals for cytological characterization. From 119 cultures from 65 patients 76 cultures from 47 patients could be analysed. Cultures stimulated with PHA and/or PWM showed in 43,4% a higher stimulation rate in comparison with cultures from normal persons. These findings are discussed in relation to the increased agar-colony growth growth of leukaemic cells after in vitro PHA-stimulation and transformation of blast cells.
D006801:Humans; D066298:In Vitro Techniques; D037102:Lectins; D015470:Leukemia, Myeloid, Acute / Q000276:immunology*; D007962:Leukocytes / Q000187:drug effects / Q000276:immunology*; D008213:Lymphocyte Activation / Q000187:drug effects; D008934:Mitogens; D013268:Stimulation, Chemical
D037102:Lectins; D008934:Mitogens
Antigenstimulation peripherer Leukozyten von Patienten mit akuten Leukämien.