Cluster characterization in iron-sulfur proteins by magnetic circular dichroism.
We report magnetic circular dichroism (MCD) spectra of 4-Fe iron-sulfur clusters in the iron-sulfur proteins Chromatium high-potential iron protein  (HIPIP), Bacillus stearothermophilus ferredoxin and Clostridium pasteurianum ferredoxin. The MCD is found to vary significantly with cluster oxidation state but is relatively insensitive to the nature of the protein. The spectra obtained are compared with the corresponding spectra of iron-sulfur proteins containing 2-Fe clusters. It is concluded that MCD is useful for the characterization of iron-sulfur cluster type and oxidation state in iron-sulfur proteins and is superior for this purpose to absorption and natural circular dichroism spectroscopy.
D002844:Chromatium / Q000032:analysis; D002942:Circular Dichroism; D003013:Clostridium / Q000032:analysis; D005288:Ferredoxins; D001411:Geobacillus stearothermophilus / Q000032:analysis; D007506:Iron-Sulfur Proteins; D008667:Metalloproteins; D010084:Oxidation-Reduction; D011487:Protein Conformation
D005288:Ferredoxins; D007506:Iron-Sulfur Proteins; D008667:Metalloproteins