title: Covalent association of protein with replicative form DNA of parvovirus H-1.
abstract: The double-stranded replicative form (RF) DNA of the autonomous parvovirus H-1 can be isolated from infected cells in a covalent complex with protein. The protein is present on most or all of the RF DNA, including actively replicating molecules, and is associated with the 5'-terminal endonuclease Hae III fragments of both the viral and complementary strands of RF. The size of the protein is estimated to be 60,000-70,000 daltons from its effect on buoyant density of DNA. DNA with covalently bound protein has not been found in H-1 virions.
mesh_terms: D002500:Centrifugation, Isopycnic; D004261:DNA Replication; D004279:DNA, Viral / Q000378:metabolism*; D003852:Deoxyribonucleoproteins / Q000378:metabolism*; D008970:Molecular Weight; D009698:Nucleoproteins / Q000378:metabolism*; D010321:Parvoviridae / Q000378:metabolism*; D014764:Viral Proteins / Q000378:metabolism; D014779:Virus Replication
chemical_list: D004279:DNA, Viral; D003852:Deoxyribonucleoproteins; D009698:Nucleoproteins; D014764:Viral Proteins