title: Rosette formation assays in dogs: lack of specificity of E rosettes for T lymphocytes.
abstract: The present study examined the specificity of guinea pig erythrocyte (E) and erythrocyte-antibody-complement (EAC) rosette formation assays with suspensions of canine peripheral blood lymphocytes. Neutrophils, monocytes, and lymphocytes bound EAC but not erythrocyte-antibody (EA) controls. Similarly, all three cell types formed rosettes with guinea pig E. Adherence of guinea pig E to these cells was apparently mediated by natural cytophilic antibodies present in the serum used in the suspension medium. The nonspecificity of the guinea pig E-rosette formation assay with canine lymphocytes renders the technique unreliable for the identification of thymus-derived lymphocytes in dogs.
mesh_terms: D000818:Animals; D000906:Antibodies; D001769:Blood; D003165:Complement System Proteins; D004285:Dogs; D004912:Erythrocytes / Q000276:immunology; D005856:Germ-Free Life; D007104:Immune Adherence Reaction; D009000:Monocytes / Q000276:immunology; D009504:Neutrophils / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000906:Antibodies; D003165:Complement System Proteins