Failure of correlation between in vitro and in vivo effect of T and B cell mitogens in the mouse.
T and B cell mitogens as PHA, PWM, LPS, SIII, DS, PVP were investigated in vitro in direct MI assay with mouse PECs as well as in vivo for their phagocytosis-enhancing capacity. All mitogens induced a dose-dependent MI although of differing degrees. In contrast, a phagocytosis-stimulating effect in vivo could be observed only by using the mitogens of bacterial origin such as LPS and SIII; the other mitogens induced, however, rather a loss of phagocytosis, although a cellular reaction in the sense of an increased PEC number was partly registered. The reactivity of PEC to LPS but not to PHA disappeared in the MI assay after foregoing desensitization of mice with the corresponding mitogen, suggesting the existence of a cellular immunity against the bacterial antigen. The importance of the induction of cell-mediated events in infection immunity in a classical immunological way will be discussed.
D000818:Animals; D001202:Ascitic Fluid / Q000276:immunology; D002464:Cell Migration Inhibition; D004305:Dose-Response Relationship, Drug; D007111:Immunity, Cellular / Q000187:drug effects*; D037102:Lectins / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000276:immunology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010587:Phagocytosis / Q000187:drug effects*; D011135:Polysaccharides, Bacterial / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000276:immunology*
D037102:Lectins; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial