Reactivation of nuclei containing lens fiber cells to mitotic growth biochemical and immunochemical analysis.
Nuclei containing lens fiber cells can be reactivated to mitotic growth by transplantation into a suitable culture medium. Reactivated epithelium-like fiber cells are morphologically indistinguishable from cultivated epithelial cells. During reactivation the enzyme pattern of the differentiated fiber cells changes to a pattern closely related to that of cultivated epithelial cells. Moreover dedifferentiation was correlated with an appreciable loss of specific lens proteins (alpha-crystallin). Cytoplasmic shedding and attachment of fiber cells to the substratum are discussed with regard to their role in the reactivation process.
D002454:Cell Differentiation; D002478:Cells, Cultured; D003459:Crystallins / Q000032:analysis; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007908:Lens, Crystalline / Q000166:cytology*; D007931:Leucyl Aminopeptidase / Q000378:metabolism; D008938:Mitosis; D011755:Pyrophosphatases / Q000378:metabolism
D003459:Crystallins; D007770:L-Lactate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D007931:Leucyl Aminopeptidase; D011755:Pyrophosphatases