Evidence that Con A induces cytotoxicity in the same subclass of T cells as does alloimmunization.
We have studied the induction of cytoxic activity in murine T cells by the T cell mitogen Con A. Here we report the results of experiments that indicate that this cytotoxicity develops in the same class of T cells potentially activatable to cytotoxicity by immunization with allogeneic cells. Cytotoxic activity does not result from activation of cells of the T-helper class by PHA, and extensive reduction of the proportion of cells of the T-helper class by in vivo treatment with ATS does not comparably reduce the cytotoxicity developed in response to Con A. The Con A-activated cytotoxic cell sediments as a large cell. Furthermore, spleen cell populations previously immunized to alloantigens in vivo develop greatly increased cytotoxicity specific for the alloantigen of the immunizing haplotype after culture with Con A in vitro.
D000818:Animals; D000961:Antilymphocyte Serum / Q000494:pharmacology; D002469:Cell Separation; D002478:Cells, Cultured; D002498:Centrifugation; D003208:Concanavalin A / Q000494:pharmacology*; D003601:Cytotoxicity Tests, Immunologic; D005260:Female; D007519:Isoantigens; D037102:Lectins / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000961:Antilymphocyte Serum; D007519:Isoantigens; D037102:Lectins; D003208:Concanavalin A