Early steroid metabolism in Xenopus laevis, Rana temporaria and Triturus vulgaris embryos.
Embryos of Xenopus laevis, Rana temporaria and Triturus vulgaris exposed to radioactive pregnenolone have been found to convert it to progesterone. Incubations with radioactive progesterone showed that it was actively metabolized by oocytes and embryos. In Xenopus incubations progesterone was converted to 5alpha-pregnane-3,20-dione, 17alpha-hydroxy-4-pregnen-3-one, 4-androstene-3,17-dione and 17alpha-20alpha-dihydroxy-4-pregne-3-one, indicating 5alpha-reductase, 17alpha-hydroxylase, 19-20-desmolase and 20alpha-hydroxylase activities. In oocytes of Triturus and Rana no evidence of 19-20-desmolase was found. In Rana oocytes were also not evidence of 17alpha-hydroxylase activity. All identified activities except 20alpha-hydroxylase were common to embryos of all three species. It is suggested that the steroid enzyme activities present in the embryos are not solely derived from the oocytes but synthentized during early development. Possible meaning of this kind of metabolism during differentiation remains open.
D000818:Animals; D001001:Anura; D002454:Cell Differentiation; D005260:Female; D006913:Hydroxysteroid Dehydrogenases / Q000378:metabolism; D008190:Lyases / Q000378:metabolism; D009865:Oocytes / Q000201:enzymology; D011284:Pregnenolone / Q000378:metabolism*; D011374:Progesterone / Q000378:metabolism*; D011896:Rana temporaria / Q000196:embryology* / Q000378:metabolism; D013254:Steroid 17-alpha-Hydroxylase / Q000378:metabolism; D013250:Steroid Hydroxylases / Q000378:metabolism; D014319:Triturus / Q000196:embryology* / Q000378:metabolism; D014981:Xenopus / Q000196:embryology* / Q000378:metabolism
D011374:Progesterone; D011284:Pregnenolone; D006913:Hydroxysteroid Dehydrogenases; D013250:Steroid Hydroxylases; D013254:Steroid 17-alpha-Hydroxylase; D008190:Lyases