The generation of killer cells to trinitrophenyl-modified allogeneic targets by lymphocyte populations negatively selected to strong alloantigens.
Negatively selected mouse and rat lymphocyte populations, specifically deprived of alloreactivity to a particular major histocompatibility complex (MHC) haplotype, are nevertheless fully capable of responding to trinitrophenyl (TNP)-modified allogeneic stimulator cells and developing cytotoxic T-lymphocyte activity to TNP-altered allogeneic target cells. As for syngeneic systems, lytic expression of those responder killer cells also requires MHC identity between the target and stimulator cell populations. Such a finding argues strongly against two variations of the dual recognition hypothesis: like-like interactions and adaptive differentiation. Instead, these data favor either the altered self model or a third variation of the dual receptor model, where one of the relevent receptors is specific for the modifying antigen and the second is a low affinity receptor unable to be triggered in the absence of a modifying antigen.
D000818:Animals; D002469:Cell Separation; D002478:Cells, Cultured; D003601:Cytotoxicity Tests, Immunologic; D005838:Genotype; D006649:Histocompatibility Antigens; D066298:In Vitro Techniques; D007519:Isoantigens; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009578:Nitrobenzenes / Q000494:pharmacology*; D051381:Rats; D011916:Rats, Inbred F344; D011919:Rats, Inbred Strains; D013601:T-Lymphocytes / Q000276:immunology*; D014301:Trinitrobenzenes / Q000494:pharmacology*
D006649:Histocompatibility Antigens; D007519:Isoantigens; D009578:Nitrobenzenes; D014301:Trinitrobenzenes