The isolation and characterisation of a plaque-forming derivative of bacteriophage Mu carrying a fragment of Tn3 conferring ampicillin resistance.
We have isolated a plaque-forming derivative of phage Mu which carries a determinant for ApR. The biological properties of this MuAp phage are similar to those of normal Mu. Its genome contains a 1.1 kb substitution where Mu DNA from the right end of the G region has been replaced by a similar length of DNA from the transposon Tn3. This fragment of Tn3 DNA carries the ApR gene, but is no longer capable of independent transposition.
D000667:Ampicillin / Q000494:pharmacology*; D001435:Bacteriophages / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D005814:Genes, Viral; D008040:Genetic Linkage; D010403:Penicillin Resistance; D010641:Phenotype; D011995:Recombination, Genetic; D014161:Transduction, Genetic; D010948:Viral Plaque Assay
D004279:DNA, Viral; D000667:Ampicillin