title: Increased phosphorylation of a specific protein in mitogen-stimulated lymphocytes.
abstract: The phosphorylation of proteins from murine splenic lymphocytes was studied. When the phosphorylation of proteins in extracts from Concanavalin A (ConA)-treated lymphocytes was compared with that of resting lymphocytes, there was only one detectable difference between them. A protein of 135,000 mol. wt was highly phosphorylatable in extracts from ConA-treated cells while phosphate incorporation into this protein was slight in extracts from untreated cells. This effect could be observed 12 h after ConA treatment and was maximal during S phase. This soluble protein was also phosphorylated in intact lymphocytes.
mesh_terms: D000818:Animals; D002474:Cell-Free System; D003208:Concanavalin A / Q000494:pharmacology; D066298:In Vitro Techniques; D007399:Interphase; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008970:Molecular Weight; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D013997:Time Factors
chemical_list: D010750:Phosphoproteins; D003208:Concanavalin A