Microcytotoxicity test in allograft immunity: specificity and kinetics of effetor T cells.
To obtain further insight into the informatory possibilities of the Takasugi-Klein microcytotoxicity test (MCT), mouse spleen cells were prepared after a single immunization with 5 X 10(7) H-2 incompatible cells and tested for reactivity against mouse fibroblasts. The following three aspects were investigated: (1) Specificity of the reaction: various mixtures of different target cells revealed absence of innocent bystander kill even after 48 h test incubation. (2) Kinetics of the reactivity after short-term (20 h) or long-term (48 h) incubation: the former disappeared rapidly, whereas the latter remained detectable beyond day 60 after immunization. This suggests the activity of different cells: an early killer cell and a later increasingly dominating effector cell which requires long-term incubation with the target. (3) Characterization of the effector cells: anti-theta-serum treatment abolished MCT reactivity completely at early and late stages after immunization. Nylon wool effluent (T-enriched) spleen cells were absolutely more active after short-term but became relatively less effective after long-term incubation as compared to nonseparated spleen cells, suggesting a higher adhesiveness of the cells which operate the 48 h reaction. The findings may demonstrate the usefulness of the MCT for in vitro studies on presence and behavior of different cells involved in allograft immunity in vivo.
D000818:Animals; D003601:Cytotoxicity Tests, Immunologic; D005347:Fibroblasts / Q000276:immunology; D006649:Histocompatibility Antigens; D007700:Kinetics; D008198:Lymph Nodes / Q000637:transplantation; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008815:Mice, Inbred Strains; D013154:Spleen / Q000637:transplantation; D013601:T-Lymphocytes / Q000276:immunology*; D013997:Time Factors; D014185:Transplantation, Isogeneic
D006649:Histocompatibility Antigens