Human peripheral blood "null-lymphocytes", a highly enriched pool for granulocytic stem cells (CFU-C).
A "Null"-lymphocyte preparation containing a high concentration of granulocytic stem cells (CFU-C) was isolated from human peripheral blood. 3 lymphocyte purification procedures were shown to produce an enrichment of CFU-C: 1. Removal of mononuclear phagocytes by iron phagocytosis and plastic adherence procedures. 2. Passage through Ig anti-Ig columns. 3. Removal of E-rosette-forming T cells. A combination of all 3 purification steps resulted in a 30- to 40-fold enrichment of CFU-C in the final "Null"-lymphocyte population as compared to the initial lymphocyte fraction isolated by Ficoll-Urografin density gradient centrifugation.
D000818:Animals; D001666:Binding Sites, Antibody; D002417:Cattle / Q000276:immunology; D002448:Cell Adhesion; D002455:Cell Division; D002469:Cell Separation / Q000379:methods*; D004912:Erythrocytes / Q000276:immunology; D006098:Granulocytes; D006412:Hematopoietic Stem Cells; D006801:Humans; D007104:Immune Adherence Reaction; D007141:Immunoglobulin Fc Fragments; D007501:Iron; D008214:Lymphocytes; D010587:Phagocytosis; D011817:Rabbits / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D007141:Immunoglobulin Fc Fragments; D007501:Iron