Determination of fluoride and chlorhexidine from chlorhexidine/fluoride-containing dentifrices.
The intention of the present experiment was to study the effects of a possible interaction between fluoride and chlorhexidine when both agents were incorporated in the same vehicle. The amount of fluoride extractable from dentrifrices containing 0.1% NaF and the fluoride ion activity were not reduced by the addition of 2% chlorhexidine digluconate. Less than 50% of the added chlorhexidine was available when the dentifrices were dissolved in deionized water. This was not affected by the presence of fluoride. The in vitro antibacterial activity of the chlorhexidine-containing dentifrices was not reduced by the addition of fluoride. Approximately 40% of the chlorhexidine was retained in the human oral cavity after brushing for 1 min with both the chlorhexidine- and the chlorhexidine/fluoride-containing dentifrice. Thus the binding of chlorhexidine to vehicle ingredients when dissolved in water is probably too weak to affect the retention in the mouth.
D001419:Bacteria / Q000187:drug effects; D002710:Chlorhexidine / Q000032:analysis* / Q000494:pharmacology; D003802:Dentifrices / Q000032:analysis*; D004347:Drug Interactions; D005460:Fluorides, Topical / Q000032:analysis* / Q000494:pharmacology; D006801:Humans; D008826:Microbial Sensitivity Tests; D009061:Mouth Mucosa / Q000378:metabolism; D012463:Saliva / Q000382:microbiology
D003802:Dentifrices; D005460:Fluorides, Topical; D002710:Chlorhexidine