title: Genital tract-to-eye infection: tissue culture of Chlamydia trachomatis.
abstract: A method of cell culture using HeLa 229 cells is described for the isolation of Chlamydia trachomatis. From 40 patients with non-specific urethritis (NSU) attending a venereal disease clinic, the isolation rate was 60 percent and 78 percent from neonates with sticky eyes from which no other organism could be isolated.
mesh_terms: D002478:Cells, Cultured; D002692:Chlamydia trachomatis / Q000302:isolation & purification / Q000648:ultrastructure; D003235:Conjunctivitis, Inclusion / Q000175:diagnosis / Q000453:epidemiology / Q000635:transmission*; D006367:HeLa Cells; D006801:Humans; D007231:Infant, Newborn; D007232:Infant, Newborn, Diseases / Q000175:diagnosis / Q000453:epidemiology; D008219:Lymphogranuloma Venereum / Q000175:diagnosis / Q000453:epidemiology / Q000635:transmission*; D008297:Male; D009520:New Zealand; D014526:Urethritis / Q000382:microbiology