Calcium fluoride containing granules produced in vitro in rat bones.
Femurs and tibias of normal rats were fixed in 4% unbuffered solutions of glutaraldehyde or formaldehyde containing 1% NaF. Following washing, radiographically controlled demineralization was performed in 3.25% HNO3. Light microscopy and transmission electron microscopy revealed the presence of so-called fluoride granules in bone trabeculae, cartilage, cartilage remnants, bone cells, chondrocytes and in the bone marrow. Selected area diffraction indicated that the fluoride granules contained CaF2. X-ray diffraction analysis of non-demineralized NaF-fixed bones were negative for CaF2. The results are discussed and related to precipitation artifacts reported in demineralized hard tissues and to similar CaF2-containing granules observed in demineralized bones and incisors of rats given high doses of NaF in diet or drinking water.
D000818:Animals; D001842:Bone and Bones / Q000032:analysis*; D002124:Calcium Fluoride / Q000032:analysis*; D002356:Cartilage / Q000032:analysis*; D005404:Fixatives; D005459:Fluorides / Q000494:pharmacology*; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D012969:Sodium Fluoride / Q000494:pharmacology*; D014961:X-Ray Diffraction
D005404:Fixatives; D012969:Sodium Fluoride; D002124:Calcium Fluoride; D005459:Fluorides