Immunologic properties of protein-lipopolysaccharide complexes. I. Antibody response of normal, thymectomized, and nude mice to a lysozyme-lipopolysaccharide complex.
The in vivo antibody response to the lysozyme component of a lysozyme-lipopolysaccharide complex has been investigated in normal, thymectomized and nude mice. The splenic PFC response elicited by the complex in CBA mice is 10- to 20-fold higher than the response elicited by lysozyme admixed with LPS. Both lysozyme-LPS complexes and lysozyme + LPS mixtures prime mice for a subsequent secondary anti-lysozyme response. In contrast, thymectomized mice responded poorly to lysozyme-LPS complexes unless reconstituted with splenic T cells. However, nude mice responded as well as Nu/+ controls to the complex. The PFC response of normal and of nude mice was severely depressed by treatment with anti-lymphocyte serum. These findings suggest that T lymphocytes contribute significantly to the enhanced immune responsiveness associated with LPS administration.
D000818:Animals; D000917:Antibody Formation; D000941:Antigens / Q000008:administration & dosage; D000961:Antilymphocyte Serum / Q000494:pharmacology; D005260:Female; D006241:Haptens; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D007156:Immunologic Memory; D008070:Lipopolysaccharides / Q000276:immunology*; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D008819:Mice, Nude; D009113:Muramidase / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D013934:Thymectomy; D013950:Thymus Gland / Q000276:immunology
D000941:Antigens; D000961:Antilymphocyte Serum; D006241:Haptens; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008070:Lipopolysaccharides; D009113:Muramidase