Preparation of high titered rabbit anti-mouse IgE sera.
A method for the consistent production of rabbit anti-mouse IgE sera is described. Rabbits were immunized intravenously with IgE-anti-normal mouse serum mixtures over 8 to 12 weeks. Ten microliters of the anti-mouse IgE sera completely neutralized all the mouse IgE in the serum (50 microliter) possessing a passive cutaneous anaphylaxis titer of 20,000.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000302:isolation & purification*; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D007073:Immunoglobulin E; D051379:Mice; D010323:Passive Cutaneous Anaphylaxis; D011817:Rabbits / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D007073:Immunoglobulin E