Quantitative determination of sodium-o-[(2,6-dichlorophenyl)-amino]phenylacetate (diclofenac) in human blood plasma or serum.
A rapid method for the estimation of sodium-(o-[(2,6-dichlorophenyl)-amino]-phenyl)-acetate (diclofenac-Na, Voltaren) used in the treatment of rheumatic diseases, in human blood plasma is described. The substance is extracted from acidified plasma with dichloromethane and acetylated after evaporation of the organic solvent, whereupon quantitation is performed by gas-liquid chromatography on a 10% OV-17 column, F.I. detection.
D000215:Acylation; D002853:Chromatography, Liquid; D004008:Diclofenac / Q000097:blood* / Q000627:therapeutic use; D006801:Humans; D010648:Phenylacetates / Q000097:blood*; D012216:Rheumatic Diseases / Q000097:blood / Q000188:drug therapy; D013997:Time Factors
D010648:Phenylacetates; D004008:Diclofenac