A rapid quantitative assay for lymphotoxin.
A rapid quantitative assay for lymphotoxin was developed with the use of a mouse cultured lymphoid cell line, L1210 as the target cell. The assay produces results which are substantially in agreement with assays employing fibroblasts as targets. After incubation with lymphotoxin containing samples, target cells were labelled with [3H]thymidine and harvested with a Multiple Automated Sample Harvester (MASH). The MASH allows multiple replicates to be obtained from which the calculation of an I50 (50% inhibition) point and lymphotoxin specific activities can be performed with high statistical reliability by means of probit transformation and analysis.
D000818:Animals; D003601:Cytotoxicity Tests, Immunologic; D004306:Dose-Response Relationship, Immunologic; D006801:Humans; D007939:Leukemia L1210 / Q000276:immunology; D008233:Lymphotoxin-alpha / Q000032:analysis* / Q000302:isolation & purification; D051379:Mice
D008233:Lymphotoxin-alpha