Responses of enriched populations of feline T and B lymphocytes to mitogen stimulation.
Lymphocytes from healthy adult cats were separated into T and B cell-enriched subfractions by centrifuging rosetted cells on sodium metrizoate/Ficoll gradients. The responsiveness of unseparated lymphocytes (T + B), and T and B cell-enriched subfractions to stimulation with mitogens (phytohemagglutinin-P (PHA-P), concanavalin A (ConA), and pokeweed mitogen (PWM) was tested. Cultures of unseparated lymphocytes and those enriched in T cells showed similar responsiveness of PHA, ConA, and PWM stimulation; however, only a weak response to ConA and PWM was observed in B cell-enriched cultures. The mitogenic effects of PHA-P, ConA, and PWM on feline lymphocytes appeared to be due primarily to T-cell activation.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D002415:Cats / Q000276:immunology*; D002469:Cell Separation; D003208:Concanavalin A / Q000494:pharmacology; D005260:Female; D037102:Lectins / Q000494:pharmacology*; D008213:Lymphocyte Activation; D008297:Male; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000276:immunology*
D037102:Lectins; D003208:Concanavalin A