Effect of inhibitory and stimulatory agents on protein synthesis in hepatomas and host livers of rats.
Female inbred BUF rats bearing intrahepatically transplanted hepatomas (5123 or 19) were subjected to acute exposure to a variety of hepatotoxic agents (actinomycin D, aflatoxin B1, CCl4, dimethylnitrosamine, ethionine, puromycin, or sparsomycin) or of stimulatory agents (hydrocortisone, phenobarbital, or whole-body X-ray). The responses in terms of changes in polyribosomes and protein synthesis (in vitro and in vivo) of host liver and hepatoma were evaluated. The responses of the host livers and hepatomas to the different agents varied. In general, the host livers responded much more than did the hepatomas. Of the two hepatomas, hepatoma 19 responded less (particularly in terms of polyribosome changes) than did hepatoma 5123. In a few experiments, different doses of actinomycin D, ethionine, or sparsomycin were used and in all instances the host livers responded more than did the hepatomas.
D000818:Animals; D005260:Female; D066298:In Vitro Techniques; D008099:Liver / Q000187:drug effects* / Q000378:metabolism* / Q000528:radiation effects / Q000648:ultrastructure; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D011132:Polyribosomes / Q000378:metabolism; D014176:Protein Biosynthesis; D051381:Rats; D013268:Stimulation, Chemical
D009363:Neoplasm Proteins