[Electrophoretic and immunologic characteristics of the Fc+ and Fc- fractions of the splenic cells of CBA mice].
Conditions have been established for the effective separation of Fc+ and Fc- cells, using sheep red blood cells monolayer, sensitized with the anti-SRBC antibodies. The method was modified by replacement of poly-L-lysine by protamine sulfate. The electrophoretic distribution profiles of Fc+ and Fc- mouse splenocytes were determined. Fc+ cells had mostly a slow electrophoretic mobility. The Fc- cells were more mobile. The Fc+ fraction contained mainly B-lymphocytes, and the Fc- fraction was enriched with T cells. There was an extensive overlap between the electrophoretic profiles of the Fc+ and Fc- splenocytes. After the splenocyte passage through the glass bead column the overlap became much less.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D001666:Binding Sites, Antibody; D002469:Cell Separation; D003602:Cytotoxicity, Immunologic; D004586:Electrophoresis; D007141:Immunoglobulin Fc Fragments; D051379:Mice; D008808:Mice, Inbred CBA; D011817:Rabbits; D013154:Spleen / Q000166:cytology / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
D007141:Immunoglobulin Fc Fragments
Elektroforeticheskaia i immunologicheskaia kharakteristika Fc+ i Fc--fraktsiĭ kletok selezenki mysheĭ CBA.