Effect of palytoxin and serotonin on murine tumor cells.
In vitro and in vivo effects of two fractions of a crude extract from the marine invertebrate Palythoa were investigated in Novikoff hepatoma and Ehrlich ascites tumor cells. These fractions, previously identified as serotonin and a partially purified toxin, inhibited thymidine incorporation into acid-precipitable material by tumor cells. Results from respiration studies indicated that the inhibitory effect was not toxic. The data suggested that: a) The partially purified toxin exerted its inhibitory effect at the membrane level; b) this inhibitory effect might have been accomplished through the Na+K+ transport mechanism, this conclusion being supported by data on the uptake of labeled amino acids by Ehrlich ascites tumor cells; and c) in an unconfirmed experiment, the partially purified toxin increased the survival time of 50% of 6 palytoxin-treated mice at least three times as long as 6 untreated control mice. All 12 mice had received 1 X 10(6) viable Ehrlich ascites tumor cells.
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D002250:Carbon Radioisotopes; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D066298:In Vitro Techniques; D008114:Liver Neoplasms, Experimental / Q000378:metabolism*; D008297:Male; D008387:Marine Toxins / Q000494:pharmacology*; D051379:Mice; D010101:Oxygen Consumption / Q000187:drug effects; D051381:Rats; D012701:Serotonin / Q000494:pharmacology*; D013936:Thymidine / Q000378:metabolism
D000596:Amino Acids; D002250:Carbon Radioisotopes; D008387:Marine Toxins; D012701:Serotonin; D013936:Thymidine