The nucleocytoplasmic distribution of 3-O-methylglucose in the amphibian oocyte.
Ultra-low temperature microdissection was employed to determine the in situ nucleocytoplasmic distribution 3-O-methylglucose in the amphibian oocyte. The nucleocytoplasmic partition ratio, Kn/c, for this non-metabolizable monosaccharide was 1.54 +/- 0.08. The observed asymmetry could be explained by the differential solubility of the solute in the water of the nucleoplasm and the cytoplasm.
D000818:Animals; D001001:Anura; D001692:Biological Transport; D002467:Cell Nucleus / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D005260:Female; D007700:Kinetics; D008757:Methylglucosides / Q000378:metabolism*; D008759:Methylglycosides / Q000378:metabolism*; D009865:Oocytes / Q000378:metabolism*; D010063:Ovum / Q000378:metabolism*; D011894:Rana pipiens
D008757:Methylglucosides; D008759:Methylglycosides