[Simple and rapid heterologous method of radioimmunochemical determination of luteinizing hormone in the blood serum of rats. 1. Basic principles of the method].
Possibilities of creation of a radioimmunochemical method (RICM) of determination of the luteinising hormone (LH) were studied. The method is based on the application in the binding system of a water-insoluble immunosorbent obtained by copolymerization of antisera with isobutyl ester of chlorocarbonic acid. The antisera were obtained by immunization of rabbits with a highly-purified LH isolated from sheep hypophyses. The method is rapid and precise, and is carried out in a single stage. Significant results of RICM in the LH determination can be obtained with the minimal two-hour incubation time. Using different amounts of the immunosorbent in the reaction mixture with the same indicator dose of I125-LH it is possible to vary the method sensitivity (from 0.05 to 5 ng/test).
D000818:Animals; D015478:Antigens, Heterophile; D001667:Binding, Competitive; D005069:Evaluation Studies as Topic; D007457:Iodine Radioisotopes; D007986:Luteinizing Hormone / Q000097:blood*; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods*; D011864:Radioimmunosorbent Test / Q000379:methods*; D051381:Rats; D012756:Sheep / Q000276:immunology
D015478:Antigens, Heterophile; D007457:Iodine Radioisotopes; D007986:Luteinizing Hormone
Prostoĭ i bystryĭ geterologichnyĭ metod radioimmunokhimicheskogo opredeleniia liuteiniziruiushchego gormona v syvorotke krovi krys. 1. Osnovy metoda