title: IgE formation in the rat following infection with Nippostrongylus brasiliensis. III. Soluble factor for the generation of IgE-bearing lymphocytes.
abstract: Normal rat bone marrow cells incubated with serum or lymph from Nippostrongylus brasiliensis (Nb)-infected rats showed an increase in the proportion of IgE-bearing cells in culture. This effect was produced in a similar fashion by cell-free supernatants (CFS) from cultures of mesenteric lymph node cells obtained from Nb-infected rats. The action of CFS on bone marrow cells appeared to be specific for the generation of IgE-bearing cells since the proportion of IgM-bearing cells in the culture did not change. The IgE-bearing cells in bone marrow cell cultures consisted of small lymphocytes, blast cells, and mast cells, and the addition of CFS to the cultures predominantly increased the number of IgE-bearing blast cells. CFS was also effective in increasing the proportion of IgE-bearing small lymphocytes in cultures of normal mesenteric lymph node cells. Removal of IgE in CFS by an anti-IgE immunosorbent did not affect the ability of CFS to generate IgE-bearing cells. The factor(s) in CFS responsible for this activity was shown to migrate with serum beta-globulins in zone electrophoresis and to possess a molecular size of between 10(4) and 2 X 10(4) m.w. The ability of CFS to generate IgE-bearing cells was diminished by treatment with the enzymes trypsin and ribonuclease A, but was unaffected by chymotrypsin.
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D001853:Bone Marrow / Q000276:immunology; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D003470:Culture Media; D005260:Female; D007073:Immunoglobulin E / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008196:Lymph / Q000276:immunology; D008198:Lymph Nodes / Q000276:immunology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D008264:Macrophages / Q000276:immunology; D009349:Nematode Infections / Q000276:immunology*; D009559:Nippostrongylus; D051381:Rats; D012995:Solubility
chemical_list: D003470:Culture Media; D008070:Lipopolysaccharides; D007073:Immunoglobulin E