title: Replacement of serum by hemolysate as growth promoter for murine leukemic and normal hemopoietic progenitor cells in culture.
abstract: The present study was undertaken to test whether lysates prepared from rat erythrocytes can replace serum as in vitro growth promoters for murine leukemic and normal hemopoietic progenitor cells (CFU-C). Normal bone marrow and three leukemic cell lines (P-1081, L-1210 and P-815) were used in all the experiments. The soft agar technique for cloning hemopoietic cells was used to quantitate cell proliferation. Addition of hemolysate to the agar medium at a final concentration of 4% promoted the growth of a maximal number of leukemic colonies, similar to the number of colonies obtained when 20% serum was added to the soft agar. As for normal CFU-C, addition of 10% hemolysate or 40% horse serum promoted the growth of comparable numbers of colonies. Rat hemolysate can therefore replace horse serum as a growth promoter of leukemic and normal CFU-C present in culture. It was also shown that rat hemolysate cannot substitute for the colony stimulating factor (CSF) needed for the cloning of CFU-C. However, addition of hemolysate, CSF and serum to the soft agar cultures promoted a potentiated rather than an additive growth of CFU-C.
mesh_terms: D000818:Animals; D001853:Bone Marrow / Q000502:physiology*; D001854:Bone Marrow Cells; D002455:Cell Division; D002460:Cell Line; D005591:Chemical Fractionation; D002999:Clone Cells; D003115:Colony-Stimulating Factors; D003470:Culture Media; D006133:Growth Substances; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006461:Hemolysis; D007942:Leukemia, Experimental; D008297:Male; D008407:Mast Cells / Q000502:physiology; D051379:Mice; D051381:Rats
chemical_list: D003115:Colony-Stimulating Factors; D003470:Culture Media; D006133:Growth Substances