Polystyrene balls as the solid-phase of a double-antibody radioimmunoassay for human serum albumin.
Polystyrene balls have been incorporated as the solid-phase of a model double-antibody radioimmunoassay for human serum albumin. Purified IgG from the secondary antiserum is adsorbed on the 6.4 mm diameter balls. The solid-phase secondary antibody is then used to separate primary antibody bound iodinated antigen from unbound antigen. The secondary antibody coated polystyrene balls are easily prepared and manipulated; several hundred sample dilutions can readily be processed in a single assay. Assay background values of 1.5% or less are consistently obtained without extensive or special washing procedures.
D000818:Animals; D000906:Antibodies; D000888:Antibodies, Anti-Idiotypic; D006736:Horses; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D007074:Immunoglobulin G; D011137:Polystyrenes; D011817:Rabbits; D011863:Radioimmunoassay / Q000379:methods; D012709:Serum Albumin / Q000276:immunology*; D012756:Sheep
D000906:Antibodies; D000888:Antibodies, Anti-Idiotypic; D007106:Immune Sera; D007074:Immunoglobulin G; D011137:Polystyrenes; D012709:Serum Albumin