Temperature-sensitive mutants of mouse hepatitis virus produce a high incidence of demyelination.
Mutagenesis of mouse hepatitis virus with 5-azacytidine or 5-fluorouracil yielded several temperature-sensitive mutants. Mutants have been isolated that dramatically enhance the production of demyelinating disease over that previously noted with the wild-type virus. This reproducible model should now make possible the precise elucidation of the pathogenic mechanism and molecular basis of this virus-induced demyelination.
D000818:Animals; D003332:Coronaviridae / Q000235:genetics; D003711:Demyelinating Diseases / Q000209:etiology* / Q000473:pathology; D004195:Disease Models, Animal; D006505:Hepatitis / Q000382:microbiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009154:Mutation; D013116:Spinal Cord / Q000473:pathology; D013696:Temperature