[Cell activation and nuclear volume (author's transl)].
Freezing dried semithin sections (1 micron) coloured with nuclear fast red are suited for determination of volume and activation state of cells and nuclei by means of electronic image analysis. Additional investigations of the proteinsynthesis with help of autoradiographs after incorporation of tritiated amino acids affirm the informations. Magnification of the nucleus together with a simultaneous increase of the proteinsynthesis per unit volume characterize the Stratum basale near the amputation level as the most active area of the regeneration epidermis. The cell volume is correlated with the alterations of the nuclear volume. Determination of the mean relative volume of nuclei with aid of measurements of nuclei sections suffices to get informations about cell activity.
D000818:Animals; D001345:Autoradiography; D002467:Cell Nucleus / Q000502:physiology*; D002468:Cell Physiological Phenomena; D004817:Epidermis / Q000378:metabolism / Q000648:ultrastructure; D007620:Karyometry; D014176:Protein Biosynthesis; D012038:Regeneration; D014319:Triturus / Q000502:physiology
Zellaktivierung und Kernvolumen.