Improved medium for clonal growth of human diploid fibroblasts at low concentrations of serum protein.
A new medium (MCDB 104) has been developed which will support clonal growth of WI-38 cells at concentrations of serum protein as low as 25 micrograms per ml (equivalent to 0.05% serum). The principal factors responsible for reduction of the protein requirement are: (a) adjustment of all nutrient concentrations in medium F12 to experimentally determined optimum values for WI-38 cells; (b) supplementation with trace elements; (c) replacement of hypoxanthine and folic acid with adenine and folinic acid; and (d) coating of the culture surface with polylysine. Individually, many of these modifications exert only a small effect on cellular growth at reduced protein concentrations, but collectively their effect has been very substantial. Other strains of fibroblast-like human diploid cells from amniotic fluid, fetal lung and newborn foreskin also will grow at reduced concentrations of serum protein in the new medium.
D000225:Adenine; D000596:Amino Acids; D000818:Animals; D001798:Blood Proteins; D002417:Cattle; D002460:Cell Line; D002999:Clone Cells; D003470:Culture Media; D004171:Diploidy; D005347:Fibroblasts; D006801:Humans; D002955:Leucovorin; D008168:Lung / Q000196:embryology; D014131:Trace Elements; D014815:Vitamins
D000596:Amino Acids; D001798:Blood Proteins; D003470:Culture Media; D014131:Trace Elements; D014815:Vitamins; D000225:Adenine; D002955:Leucovorin