In vitro RNA-RNA splicing in adenovirus 2 mRNA formation.
"Splicing" of the precursor to an adenovirus mRNA was accomplished in isolated cell-free extracts. Nuclei were prepared from hypotonically swollen cells that had been labeled with [3H]uridine for 10 min prior to nuclear isolation. Addition of a "cytoplasmic" fraction was required for the splicing to occur. The nuclear precursor, a poly(A)-terminated RNA molecule approximately 5 kilobases long, contained sequences complementary to the 58.5--75.9 region of the adenovirus 2 genome, including those sequences spliced out of the mature mRNA molecule. The in vitro spliced product was a poly(A)-terminated RNA molecule identical in size to the cytoplasmic 72,000 Mr protein mRNA (2 kilobases long) in which the sequences encoded in the 70.7--75.9 region of the viral genome were spliced to those encoded at 58.7--65.6, with the sequences encoded at 66.1--70.7 deleted.
D000260:Adenoviruses, Human / Q000378:metabolism*; D001483:Base Sequence; D002467:Cell Nucleus / Q000378:metabolism; D006367:HeLa Cells / Q000378:metabolism; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis*; D012367:RNA, Viral / Q000378:metabolism*; D014158:Transcription, Genetic; D014529:Uridine / Q000378:metabolism
D012333:RNA, Messenger; D012367:RNA, Viral; D011061:Poly A; D014529:Uridine