Monoclonal antibodies reacting with murine teratocarcinoma cells.
Monoclonal antibodies were produced in vitro by fusing mouse myeloma cells with spleen cells from a rat immunized with the C3H mouse teratocarcinoma C86-S1. After the fusion two clones were chosen for further analysis. The first clone, 3C4-10, produced an antibody recognizing an antigen with a distribution restricted to teratocarcinoma cell lines, an endoderm cell line, and a neuroblastoma. The second clone, 4A1-9, produced an antibody that reacted with all cultured murine cells tested and adult brain. Neither antibody reacted with preimplantation embryos. The 3C4-10 antibody recognized an antigen associated with proteins. The apparent molecular weight of the 3C4-10 antigen was greater than 100,000.
D000818:Animals; D000912:Antibodies, Neoplasm; D000918:Antibody Specificity; D001921:Brain / Q000276:immunology; D002454:Cell Differentiation; D002999:Clone Cells / Q000276:immunology; D003429:Cross Reactions; D010064:Embryo Implantation; D004707:Endoderm / Q000276:immunology; D005260:Female; D006822:Hybrid Cells / Q000276:immunology; D051379:Mice; D009374:Neoplasms, Experimental / Q000276:immunology; D009447:Neuroblastoma / Q000276:immunology; D013724:Teratoma / Q000276:immunology*
D000912:Antibodies, Neoplasm