Effect of isoprenaline on salivary gland lipids of rats fed a choline-deficient diet.
Male adult rats were fed choline deficient and a choline-supplemented diet for 3 weeks. Half the rats from each group were injected with isoprenaline (16 mg/kg body weight) and the remainder with saline. After 2 and 8 hours, rats were sacrificed, their submandibular salivary glands were disected out, and extracted for lipids. The fatty acid composition of total lipids and some neutral and phospholipid fractions were determined. Isoprenaline administration resulted in a slightly higher level of oleic acid in SMSG of rats fed choline-deficient but not the control diet. There was an increase in the free fatty acid (FFA) concentrations of the gland after 2 hours of isoprenaline treatment in rats fed the control diet; no such increase was observed in the choline-deficient group. The fatty acid composition of FFA fraction was also slightly changed as a result of isoprenaline treatment, but only in the choline-deficient group. The fatty acid composition of triglyceride (TG), phosphatidyl choline (PC), and phosphatidyl ethanolamine (PE) fractions was not changed.
D000818:Animals; D002796:Choline Deficiency / Q000378:metabolism*; D004032:Diet; D005227:Fatty Acids / Q000032:analysis*; D007545:Isoproterenol / Q000494:pharmacology*; D008055:Lipids / Q000032:analysis*; D008297:Male; D010713:Phosphatidylcholines / Q000032:analysis; D010714:Phosphatidylethanolamines / Q000032:analysis; D010743:Phospholipids / Q000032:analysis; D051381:Rats; D013363:Submandibular Gland / Q000032:analysis / Q000187:drug effects* / Q000378:metabolism; D014280:Triglycerides / Q000032:analysis; D014806:Vitamin B 12 Deficiency / Q000378:metabolism
D005227:Fatty Acids; D008055:Lipids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D014280:Triglycerides; D007545:Isoproterenol