Induction of ornithine decarboxylase activity in a temperature-sensitive cell cycle mutant of Chinese hamster cells.
We have investigated the induction of ornithine decarboxylase (L-ornithine carboxy-lyase, EC 4.1.1.17) activity in a temperature-sensitive cell cycle mutant of Chinese hamster fibroblasts. This activity is not induced at the nonpermissive temperature, although the synthesis of the majority of proteins is normal. From a combination of studies with inhibitors of mRNA synthesis and maturation (alpha-amanitin, and cordycepin) and of proteins synthesis (cycloheximide, diphtheria toxin, and emetine), we conclude that the temperature-sensitive block is at the level of translation of one or more specific mRNAs.
D002262:Carboxy-Lyases / Q000096:biosynthesis*; D002453:Cell Cycle; D002478:Cells, Cultured; D004790:Enzyme Induction; D009154:Mutation; D009955:Ornithine Decarboxylase / Q000096:biosynthesis* / Q000235:genetics; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D013696:Temperature; D014158:Transcription, Genetic
D012333:RNA, Messenger; D002262:Carboxy-Lyases; D009955:Ornithine Decarboxylase