Cytotoxicity of radiosensitizers in multicell spheroids: combination treatment with hyperthermia.
Spheroids of EMT6 tumour cells were grown in spinner culture. As with V79 cell spheroids, the combination of 1.5mM misonidazole and hyperthermia at 42.5 degree C for 1 or 2 h cuased greater cytotoxicity than either heat or drug alone. Radioresistant hypoxic cells at the centre of the spheroids were eliminated but other cells were also killed.
D000818:Animals; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D006358:Hot Temperature; D008325:Mammary Neoplasms, Experimental; D008954:Models, Biological; D009593:Nitroimidazoles / Q000494:pharmacology*; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*; D013997:Time Factors
D009593:Nitroimidazoles; D011838:Radiation-Sensitizing Agents