[Kinetic analysis of mechanisms of Na+-dependent transport of organic acids in the proximal tubules of surviving frog kidney. I. Transport of fluorescein and uranin in a sodium-free environment].
By direct microfluorimetry, the kinetic characteristics of active transport system for organic acid were studied. A removal of sodium from the incubation medium is shown to affect the affinity between a carrier and acids (KS), and the rate of translocation of the transporting complex (which includes a carrier and a substrate) across the plasma membrane. The data obtained are in good accordance with the concept of Na+-dependent transport.
D000818:Animals; D001001:Anura; D001693:Biological Transport, Active / Q000187:drug effects; D005452:Fluoresceins / Q000378:metabolism*; D007687:Kidney Tubules, Proximal / Q000378:metabolism*; D007700:Kinetics; D011896:Rana temporaria; D012964:Sodium / Q000494:pharmacology*
D005452:Fluoresceins; D012964:Sodium
Kineticheskiĭ analiz mekhanizmov Na+-zavisimogo transporta organicheskikh kislot v proksimal'nykh kanal'tsakh perezhivaiushcheĭ pochki liagushki. I. Transport fluorestseina i uranina v beznatrievoĭ srede.