title: Stability of alpha and beta globin messenger RNA during induced differentiation of mouse erythroleukemia cells.
abstract: Murine erythroleukemia cells (MELC) are induced to express erythroid differentiation when cultured with hexamethylene bisacetamide (HMBA). Newly synthesized alpha and beta globin mRNA are both relatively stable, half-life (t1/2) greater than 50 hr, early in the course of induced differentiation. In fully induced cells there is a decrease in stability of both newly synthesized alpha and beta globin mRNA. The decay of alpha mRNA is faster, (t 1/2, 10--12 hr) than beta globin mRNA (t1/2, 20--22 hr). Thus, differences in stability of alpha and beta globin mRNA plays a role in determining the ratio of alpha to beta mRNA content in differentiated erythroid cells.
mesh_terms: D000081:Acetamides / Q000494:pharmacology; D000818:Animals; D002454:Cell Differentiation; D002999:Clone Cells; D003959:Diamines / Q000494:pharmacology; D004355:Drug Stability; D005914:Globins / Q000378:metabolism*; D006824:Hybridization, Genetic; D004915:Leukemia, Erythroblastic, Acute / Q000473:pathology*; D051379:Mice; D008811:Mice, Inbred DBA; D012333:RNA, Messenger / Q000096:biosynthesis / Q000378:metabolism*; D013997:Time Factors
chemical_list: D000081:Acetamides; D003959:Diamines; D012333:RNA, Messenger; D005914:Globins; C014026:hexamethylene bisacetamide