In vitro regulation of a mouse myelomonocytic leukemia line adapted to culture.
Regulation of the growth and production of granulocyte colony-stimulating activity (CSA) by WEHI-3 cells, a lysozyme-secreting mouse cell line adapted to culture, was investigated in vitro. WEHI-3 cloning efficiency is not enhanced by exogenously added CSA. However, WEHI-3 cloning efficiency in agar was suppressed by an activity in human polymorphonuclear neutrophil extract (colony-inhibiting activity) which inhibits endogenous WEHI-3 CSA production. The addition of increasing concentrations of WEHI-3- or L cell-conditioned medium containing CSA to CIA-depressed WEHI-3 agar cultures resulted in graded increases of cloning efficiency to that of the untreated sample. Testosterone, Deca-Durabolin, and bacterial lipopolysaccharide increased production of CSA by WEHI-3 cells and overcame colony-inhibiting activity-mediated suppression of CSA production, even when activating agents were added 1 day after the addition of colony-inhibiting activity. The activating agents had no direct stimulatory effect on normal mouse marrow colony-forming cells and did not enhance CSA activity. WEHI-3 cells respond to growth inhibitory and stimulatory activities and can serve as an in vitro model for studying the regulation of neoplastic cells.
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D002999:Clone Cells / Q000187:drug effects; D003115:Colony-Stimulating Factors / Q000096:biosynthesis* / Q000494:pharmacology; D006023:Glycoproteins / Q000096:biosynthesis*; D006098:Granulocytes / Q000187:drug effects; D006410:Hematopoiesis / Q000187:drug effects; D007942:Leukemia, Experimental / Q000378:metabolism; D007951:Leukemia, Myeloid / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D009277:Nandrolone / Q000031:analogs & derivatives / Q000494:pharmacology; D011135:Polysaccharides, Bacterial / Q000494:pharmacology; D013739:Testosterone / Q000494:pharmacology
D003115:Colony-Stimulating Factors; D006023:Glycoproteins; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D013739:Testosterone; D009277:Nandrolone