Simultaneous effects of erythropoietin and colony-stimulating factor on bone marrow cells.
Erythropoietin or colony-stimulating factor, or both, were added to rat or mouse marrow cell cultures, and the responses to each inducer were measured. Colony-stimulating factor caused the suppression of erythropoietin-stimulated hemoglobin synthesis, and erythropoietin caused the suppression of the granulocyte-macrophage colony formation that is dependent on colony-stimulating factor. The extent of suppression by each inducer was dose-dependent. Marrow cells from plethoric rats were more sensitive to suppression of erythropoietin action by colony-stimulating factor than were normal marrow cells. These findings suggest that either (i) the receptors for erythropoietin and for colony-stimulating factor have overlapping specificities and that the "wrong" inducer may bind without having an inductive effect, or (ii) the target cells for erythropoietin and colony-stimulating factor are very closely related or are the same.
D000818:Animals; D001853:Bone Marrow / Q000378:metabolism / Q000502:physiology*; D001854:Bone Marrow Cells; D003115:Colony-Stimulating Factors / Q000502:physiology*; D004921:Erythropoietin / Q000502:physiology*; D006023:Glycoproteins / Q000502:physiology*; D006098:Granulocytes / Q000502:physiology; D006410:Hematopoiesis; D006454:Hemoglobins / Q000096:biosynthesis; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D051381:Rats
D003115:Colony-Stimulating Factors; D006023:Glycoproteins; D006454:Hemoglobins; D004921:Erythropoietin