Effect of DMN, phenobarbital and halothane on the sedimentation characteristics of rat liver DNA.
Viable rat liver cells were isolated, and the velocity sedimentation characteristics of DNA centrifuged through alkaline sucrose gradients were studied following the administration in vivo of dimethylnitrosamine (DMN), phenobarbital and halothane. DMN application resulted in a strong decrease of sedimentation velocity, indicating generation of single strand breaks in DNA, whereas phenobarbital and halothane given in doses sufficient to induce liver enlargement did not alter the sedimentation behaviour of DNA from parenchymal or extraparenchymal liver cells.
D000818:Animals; D002499:Centrifugation, Density Gradient; D004247:DNA / Q000378:metabolism*; D004128:Dimethylnitrosamine / Q000494:pharmacology*; D006221:Halothane / Q000494:pharmacology*; D006498:Hepatectomy; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009602:Nitrosamines / Q000494:pharmacology*; D010634:Phenobarbital / Q000494:pharmacology*; D051381:Rats
D009602:Nitrosamines; D004247:DNA; D004128:Dimethylnitrosamine; D006221:Halothane; D010634:Phenobarbital