title: Rapid ATP assays in perfused mouse liver by 31P NMR.
abstract: 31P NMR was used to continuously monitor ATP and inorganic phosphate levels in perfused mouse liver. Under "optimal" conditions, the time resolution of the technique was approximately 1 min. In the absence of any metabolic perturbations the ATP level remained constant for at least 2 hr and decreased by only approximately 20% in 18 hr. Both ATP and inorganic phosphate levels responded to alterations in the oxygen supply to the liver. The half-time for this response was approximately 1 min, and the response to short periods of hypoxia or ischemia was partially reversible. The addition of insulin caused only a minor decrease in the ATP level but significantly decreased the rate of response of ATP and phosphate levels to hypoxia and ischemia.
mesh_terms: D000227:Adenine Nucleotides / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000032:analysis / Q000378:metabolism*; D000818:Animals; D005947:Glucose / Q000378:metabolism; D000860:Hypoxia / Q000378:metabolism; D007328:Insulin / Q000494:pharmacology; D007511:Ischemia / Q000378:metabolism*; D008099:Liver / Q000032:analysis / Q000098:blood supply / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008297:Male; D051379:Mice; D010710:Phosphates / Q000378:metabolism
chemical_list: D000227:Adenine Nucleotides; D007328:Insulin; D010710:Phosphates; D000255:Adenosine Triphosphate; D005947:Glucose