title: The demonstration of in vivo misonidazole tumour toxicity using post radiation hypoxia.
abstract: The cytotoxic effect of the radiosensitizer misonidazole on hypoxic tumour cells in vivo has been studied. Using standard procedures of large single doses of X-ray (30 Gy) followed by 1.0 mg/g misonidazole, no significant cytotoxicity (measured by tumour regrowth) could be observed. In contrast, when utilizing post radiation hypoxia (3 h of 7% oxygen breathing) in conjunction with post radiation misonidazole, an increased amount of direct drug toxicity was demonstrated. The use of this acute hypoxia treatment may prove to be a useful tool for measuring various in vivo radiobiological responses.
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D008325:Mammary Neoplasms, Experimental / Q000532:radiotherapy*; D051379:Mice; D009593:Nitroimidazoles / Q000494:pharmacology*; D010100:Oxygen; D011838:Radiation-Sensitizing Agents; D013997:Time Factors
chemical_list: D009593:Nitroimidazoles; D011838:Radiation-Sensitizing Agents; D010100:Oxygen