Biochemical characterization of alien H-2 antigens expressed on a methylcholanthrene-induced tumor.
A methylcholanthrene-induced tumor of BALB/c (H-2d) origin had been shown to express H-2 antigens normally associated with the H-2k haplotype. The molecules expressing the alien antigens have been partially purified and shown to be distinct from molecules expressing normal H-2d antigens. Like normal H-2 antigens, the alien antigens have a molecular weight of 48,000, are glycoproteins, and are noncovalently bound to beta 2-microglobulin. The alien antigens differ from normal H-2 antigens in their susceptibility to papain digestion, behavior during gel filtration, and overall stability. Possible mechanisms accounting for the expression of the alien antigen are discussed.
D000818:Animals; D000951:Antigens, Neoplasm / Q000302:isolation & purification; D000954:Antigens, Surface / Q000302:isolation & purification; D002462:Cell Membrane / Q000276:immunology; D006649:Histocompatibility Antigens / Q000302:isolation & purification; D008748:Methylcholanthrene; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D009374:Neoplasms, Experimental / Q000276:immunology
D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D006649:Histocompatibility Antigens; D008748:Methylcholanthrene