title: Enhancing effect of low dose cyclophosphamide treatment on the in vitro antibody response.
abstract: We have studied the effect of cyclophosphamide (CY) administration on the subsequent in vitro antibody response in the mouse. Treatment with a low dose (20 mg/kg) of CY four days before culture results in an increased IgM response to the T-independent antigen trinitrophenylated polyacrylamide (TNP-PAA), without affecting the background response of unstimulated cultures. This suggests that CY treatment eliminates a short-lived suppressor cell, involved in the regulation of the in vitro B cell response. In contrast, the same regimen decreases the ability of nude mouse spleen cells to respond to TNP-PAA, showing that the target of CY-enhancing effect is a mature T cell. The increased response observed in conventional mice should be the result of a balance between the direct suppressive effect of CY on B cells and the elimination of a suppresor T cell, the latter phenomenon being of predominant significance in our conditions. The target of CY-enhancing effect is nonadherent to plastic, but adherent to Sephadex G-10 columns.
mesh_terms: D000178:Acrylamides / Q000276:immunology; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects*; D001402:B-Lymphocytes / Q000276:immunology; D002448:Cell Adhesion; D002850:Chromatography, Gel; D003520:Cyclophosphamide / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D051379:Mice; D008811:Mice, Inbred DBA; D008819:Mice, Nude; D013154:Spleen / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology; D014301:Trinitrobenzenes / Q000276:immunology
chemical_list: D000178:Acrylamides; D014301:Trinitrobenzenes; D003520:Cyclophosphamide