Separation of helper and suppressor T lymphocytes. II. Ly phenotypes and lack of DNA synthesis requirement for the generation of concanavalin A helper and suppressor cells.
Using a Ficoll velocity sedimentation gradient, we have been able to fractionate concanavalin A (Con A)-induced helper and suppressor cells into separate pools. Cells activated by Con A to mediate helper activity are Ly1+, do not require DNA synthesis for induction, and remain as small cells after activation. Suppressor cells are Ly23+, are found in the blast cell fraction and their induction is not inhibitable by prior treatment with mitomycin C or irradiation, both of which inhibit DNA synthesis. The implications of such findings are discussed.
D000818:Animals; D002469:Cell Separation; D003037:Cobalt Radioisotopes; D003208:Concanavalin A / Q000494:pharmacology*; D004247:DNA / Q000096:biosynthesis*; D007165:Immunosuppression Therapy; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008937:Mitomycins / Q000494:pharmacology; D010641:Phenotype; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
D003037:Cobalt Radioisotopes; D008937:Mitomycins; D003208:Concanavalin A; D004247:DNA