title: Polyploidy, plants, and electrophoresis.
abstract: Investigations of polyploidy using electrophoresis are at present severely limited by several areas of difficulty which limit all applications of this technique. The technical problems inherent in electrophoresis of plants, namely extraction of active extracts and maximizing resolution of electrophoretic variants through investigation of gel and especially assay conditions, have never been addressed explicitly. No rationale for initiating work on a new species is available. Analytical approaches to defining the conditions which limit resolution are rare. Gel-to-gel variation is poorly controlled, seldom monitored. The individual nature of electrophoretic investigations limits the comparability of data among labs. In short, "hit-and-miss" approaches predominate, and these limit investigation. Techniques are now available which one can hope will greatly improve the experimental situation.
mesh_terms: D000828:Animal Population Groups / Q000235:genetics; D000818:Animals; D004586:Electrophoresis / Q000379:methods; D005819:Genetic Markers; D010944:Plants / Q000235:genetics*; D011123:Polyploidy; D012107:Research Design
chemical_list: D005819:Genetic Markers