Lysosomal composition in cultured vascular smooth muscle cells: electron probe analysis.
Spherical electron-dense organelles in the perinuclear region of cultured guinea pig aortic smooth muscle cells were identified as lysosomes by their ability to accumulate acridine orange and by cytochemical demonstration of their acid phosphatase content. The number and size of lysosomes increased in subcultured cells. The elemental composition of the lysosomes was quantitated by electron probe analysis of whole freeze-dried cells and of cryosections. In lysosomes at this stage in their development, the sulfur concentration was higher than that in the cytoplasm and the K/Na concentration ratio was similar to that in the cytoplasm.
D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D001011:Aorta / Q000032:analysis / Q000648:ultrastructure; D002478:Cells, Cultured; D004577:Electron Probe Microanalysis; D006168:Guinea Pigs; D007477:Ions / Q000032:analysis; D008247:Lysosomes / Q000032:analysis* / Q000201:enzymology; D009130:Muscle, Smooth / Q000032:analysis* / Q000648:ultrastructure
D007477:Ions; D000135:Acid Phosphatase