Comparison of the carbohydrate and amino acid composition of normal and S-variant alpha-1-antitrypsin.
alpha-1-Antitrypsin has been isolated and purified from the serum of an individual with the Pi S phenotype whose serum contains only 50--60% as much alpha-1-antitrypsin as normal M-type serum. The preparation was homogeneous by the criteria of sodium dodecyl sulfate polyacrylamide gel electrophoresis and sedimentation equilibrium ultracentrigufation. When analyzed in the ultracentrifuge, the S-type alpha-1-antitrypsin exhibited a molecular weight of 47,500 which was essentially the same as that of the M-type (47,300) and the Z-type (47,500) alpha-1-antitrypsin. The S-type alpha-1-antitrypsin contains 15.2% carbohydrate consisting of 16.4 residues/mol of N-acetylglucosamine, 7.8 residues/mol of mannose. 6.7 residues/mol of galactose and 7.1 residues/mol of sialic acid which is essentially the same as the carbohydrate composition of the M-type alpha-1-antitrypsin. In addition, M- and S-type alpha-1-antitrypsin have very similar amino acid compositions.
D000596:Amino Acids / Q000032:analysis; D002241:Carbohydrates / Q000032:analysis; D014644:Genetic Variation; D006801:Humans; D008970:Molecular Weight; D010641:Phenotype; D000515:alpha 1-Antitrypsin / Q000235:genetics
D000596:Amino Acids; D002241:Carbohydrates; D000515:alpha 1-Antitrypsin