Inhibition of growth of 3T3 cells by extract of surface membranes.
The growth of Swiss 3T3 cells can be inhibited by the addition of a plasma membrane enriched fraction from 3T3 cells [Whittenberger, B & Glaser, L. (1977) Proc. Natl. Acad. Sci. USA 74, 2251--2255]. We report conditions for the extraction of the inhibitory components from the membranes with octylglucoside. The solubilized components reversibly inhibit the rate of cell division of 3T3 but not of simian virus 40-transformed 3T3 cells. The inhibitory activity is heat labile and nondialyzable. The inhibition observed with the soluble extract can be reversed by mitogenic factors present in serum.
D002455:Cell Division; D002460:Cell Line; D002462:Cell Membrane / Q000502:physiology*; D004247:DNA / Q000096:biosynthesis; D004261:DNA Replication / Q000187:drug effects; D007700:Kinetics; D014020:Tissue Extracts / Q000494:pharmacology
D014020:Tissue Extracts; D004247:DNA