title: A gas chromatographic method for the evaluation of the vinyl epoxidase activity.
abstract: Two alternative specific and very sensitive methods for determination of vinyl epoxide synthetase activity in liver microsomes are described. Trichloroethylene, which is used as a substrate, is converted into trichloroethylene oxide by a hepatic epoxide synthetase. Chloral hydrate, the final rearrangement product of trichloroethylene oxide, is evaluated by electron capture gas chromatography, either after derivatization with pentafluorophenyl-hydrazine or after its conversion into chloroform under alcaline conditions. The kinetic parameters of the epoxidative reaction have been determined on rat hepatic microsomal suspensions.
mesh_terms: D000818:Animals; D002697:Chloral Hydrate / Q000032:analysis; D002725:Chloroform / Q000032:analysis; D002849:Chromatography, Gas; D004852:Epoxy Compounds; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D008722:Methods; D008862:Microsomes, Liver / Q000201:enzymology; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism*; D051381:Rats; D014753:Vinyl Compounds / Q000378:metabolism*
chemical_list: D004852:Epoxy Compounds; D014753:Vinyl Compounds; D002697:Chloral Hydrate; D002725:Chloroform; D006899:Mixed Function Oxygenases; D010088:Oxidoreductases