Soluble biospecific macromolecule for purification of estrogen receptor.
An alternative to affinity chromatography purification of proteins based on the use of a water-soluble biospecific polymer has been applied to the estrogen receptor from calf uterus. The receptor (4S-trypsin form) was bound to a dextran-estradiol conjugate (molecular weight approximately 500,000) and the complex was isolated by gel filtration. Highly purified receptor was subsequently released from the dextran-estradiol conjugate by exchange with [3H]estradiol.
D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D002846:Chromatography, Affinity / Q000379:methods; D002850:Chromatography, Gel / Q000379:methods; D003600:Cytosol / Q000032:analysis; D003911:Dextrans; D004958:Estradiol; D005260:Female; D011960:Receptors, Estrogen / Q000302:isolation & purification*; D012995:Solubility; D014599:Uterus / Q000032:analysis
D003911:Dextrans; D011960:Receptors, Estrogen; D004958:Estradiol