Lymphokines of T and B cells. I. Demonstration that T and B cells of tumor antigensensitised mice produce different lymphokines changing surface charge of target cells.
Lymphocytes from MC sarcoma-bearing mice were exposed to a soluble tumor antigen. The lymphokine-containing supernatant thus obtained was fractionated on Sephadex G-75. Fractions were tested for their activity to reduce the surface charge of indicator macrophages by cell electrophoresis technique. When unseparated spleen or lymph node cells were used the charge-reducing activity of the supernatant was found in three regions according to molecular weights of about 100,000, 60,000 - 35,000 and 13,000 Daltons. The appearance of activity was found to be time-dependent. As indicated by tests with separated lymphocytes both B and T cells appear to release charge-reducing activity.
D000818:Animals; D000951:Antigens, Neoplasm / Q000008:administration & dosage*; D001402:B-Lymphocytes / Q000276:immunology*; D002462:Cell Membrane / Q000276:immunology / Q000502:physiology; D004586:Electrophoresis; D066298:In Vitro Techniques; D008222:Lymphokines / Q000096:biosynthesis* / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects; D008564:Membrane Potentials; D051379:Mice; D008808:Mice, Inbred CBA; D012513:Sarcoma, Experimental / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
D000951:Antigens, Neoplasm; D008222:Lymphokines