Interferon action: two distinct pathways for inhibition of protein synthesis by double-stranded RNA.
Double-stranded RNA inhibits protein synthesis in at least two ways. It activates a protein kinase that blocks peptide chain initiation by phosphorylating the peptide chain initiation factor eIF-2 and also activates an endonuclease that inactivates different mRNAs at different rates. The protein kinase and the endonuclease have been partially purified from interferon-treated Ehrlich ascites tumor cells. The 2',5'-oligoadenylates [pppA(2'p5'A)n], found found earlier to be mediators in the activation of the endonuclease by double-stranded RNA, are not mediators in the activation of the protein kinase by double-stranded RNA.
D000227:Adenine Nucleotides / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000494:pharmacology*; D002460:Cell Line; D004720:Endonucleases / Q000378:metabolism; D004789:Enzyme Activation; D007372:Interferons / Q000494:pharmacology*; D009843:Oligoribonucleotides / Q000378:metabolism; D010448:Peptide Initiation Factors / Q000378:metabolism*; D010766:Phosphorylation; D014176:Protein Biosynthesis / Q000187:drug effects*; D011494:Protein Kinases / Q000378:metabolism*; D012313:RNA / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism
D000227:Adenine Nucleotides; D009843:Oligoribonucleotides; D010448:Peptide Initiation Factors; D012333:RNA, Messenger; C018445:adenosine triphosphate-2',5'-adenosine monophosphate; D012313:RNA; D000255:Adenosine Triphosphate; D007372:Interferons; D011494:Protein Kinases; D004720:Endonucleases