L-asparagine requirements of human T-lymphocytes and B-lymphocytes in culture.
The characterization of two human T-lymphocyte lines revealed that they required exogenous L-asparagine for cell growth, whereas all four B-cell lines studied were L-asparagine independent. T-cells were 800-2,000 times more sensitive to Escherichia coli L-asparaginase than were B-cells. The cytotoxic effects of a high concentration of L-asparaginase on B-cells were not related to the hydrolysis of L-asparagine but were due to heat-labile and heat-resistant substances in the enzyme. The findings were consistent with reports that L-asparaginase is effective in suppressing cellular immunity and inducing remission in patients with acute lymphocytic leukemia, mainly a non-B-cell disease. Thus these cell lines provide in vitro models for the study of a nutritional approach to chemotherapy or immunotherapy.
D000818:Animals; D001215:Asparaginase / Q000494:pharmacology; D001216:Asparagine / Q000378:metabolism*; D001402:B-Lymphocytes / Q000378:metabolism*; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D006801:Humans; D007942:Leukemia, Experimental / Q000188:drug therapy / Q000378:metabolism*; D007945:Leukemia, Lymphoid / Q000188:drug therapy / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism*
D001216:Asparagine; D001215:Asparaginase