Segregation of rat chromosomes in somatic cell hybrids between rat cells and HT 1080 human fibrosarcoma cells.
We produced somatic cell hybrids between HT 1080-6TG human fibrosarcoma cells and either rat white blood cells (WBC) or cells directly derived from rat spleen. Karyologic and isozyme analyses of hybrid cells indicated that they preferentially lose rat chromosomes. Hypoxanthine-aminopterine thymidine-selected hybrid clones expressing rat hypoxanthine phosphoribosyltransferase (HPRT), glucose-6-phosphate dehydrogenase (G6PD), and phosphoglycerate kinase (PGK) and containing the rat X chromosome were counterselected in a medium containing 30 micrograms/ml of 6-thioguanine. Concordant loss of the rat X chromosome and of the expression of rat HPRT and G6PD was observed in the hybrid clones.
D000818:Animals; D002460:Cell Line; D002874:Chromosome Mapping; D002875:Chromosomes; D005354:Fibrosarcoma / Q000648:ultrastructure*; D005819:Genetic Markers; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics; D006801:Humans; D006822:Hybrid Cells / Q000648:ultrastructure*; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics; D007527:Isoenzymes / Q000235:genetics; D007962:Leukocytes / Q000648:ultrastructure; D010735:Phosphoglycerate Kinase / Q000235:genetics; D051381:Rats / Q000235:genetics; D013154:Spleen / Q000166:cytology
D005819:Genetic Markers; D007527:Isoenzymes; D005954:Glucosephosphate Dehydrogenase; D007041:Hypoxanthine Phosphoribosyltransferase; D010735:Phosphoglycerate Kinase