Effect of skeletal muscle denervaton on the activity of muscular acetylcholinesterase (E.C. 3.1.) in frog.
The activity of acetylcholinesterase (AChE 3.1.1.7.) was determined in skeletal muscle homogenates in frogs at various time intervals (15--24 days) after denervation. At the same time changes in AChE activity were compared with morphological changes of neuromuscular end plates in these muscles. Muscle denervation caused initially, within 30 hours, a rise in AChE activity by about 30% in relation to control muscles, followed by its fall to the control values after 4--6 days. The activity decreased further reaching lowest values 15 days after denervation, when it amounted to about 65% of the value of control muscles. After that time, when regeneration of nerves set in, the activity of AChe rose again returning to the control values after 24 hours. In the initial period of denervation no correlation was found between AChE activity and morphological changes in end plates. It was found however in the later period when degenerative changes were most pronounced as well as when reinnervation was in progress.
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D001001:Anura; D003714:Denervation; D009045:Motor Endplate / Q000201:enzymology; D009132:Muscles / Q000201:enzymology* / Q000294:innervation*; D011896:Rana temporaria; D013997:Time Factors
D000110:Acetylcholinesterase