In vitro synthesis of alpha-1-antitrypsin in long term monolayer human liver cell cultures.
Explants for long term monolayer liver cell cultures were taken from individuals having PiM or PiZ phenotypes. Radioactive labelling, using I125 and an original method, permitted the quantitative measurement of AAT in vitro and more important, allowed Pi phenotype determination.
D002478:Cells, Cultured; D002648:Child; D006801:Humans; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008103:Liver Cirrhosis / Q000473:pathology; D010641:Phenotype; D000515:alpha 1-Antitrypsin / Q000096:biosynthesis*
D000515:alpha 1-Antitrypsin