title: Phosphorylation of specific, distinct proteins in synaptosomes and axons from squid nervous system.
abstract: Synaptosomes and axons from squid were incubated with [gamma-(32)P]ATP or [(32)P]orthophosphate and specific, distinct proteins were found to be labeled in each preparation. In axoplasm, only the major 200,000 M(r) neurofilament protein and a specific protein of approximately 400,000 M(r) were labeled, as reported previously [Pant, H. C., Shecket, G., Gainer, H. & Lasek, R. J. (1978) J. Cell Biol. 78, R23-R27]. These results were independent of whether the cosubstrates were (32)PO(4) (2-) or [gamma-(32)P]ATP. However, synaptosomes lacked the 200,000 M(r) neurofilament protein and several lower molecular weight proteins were labeled instead, the most prominent being a 47,000 M(r) species. [gamma-(32)P]ATP was much more effective in labeling the 47,000 M(r) species than (32)PO(4) (2-). Synaptosomes also contained a distinct 250,000 M(r) protein species which, however, was not labeled. The protein kinase activity in synaptosomes was sensitive to various pharmacological agents, depending on whether the labeled phosphate came directly from ATP or orthophosphate. Carbonyl cyanide p-trifluoromethoxyphenyl hydrazone, a mitochondrial H(+) uncoupler, almost completely inhibited incorporation of (32)P into protein with (32)PO(4) (2-) as cosubstrate, as expected, but produced only 32% inhibition with [gamma-(32)P]ATP as cosubstrate. The activity could be augmented by incubating synaptosomes in a calcium-free medium and could be suppressed by increasing intrasynaptosomal Ca(2+) with A23187, a Ca(2+) ionophore. The latter effect was more prominent with (32)PO(4) (2-) than with [gamma-(32)P]ATP as cosubstrate. Depolarizing agents such as veratridine and high K(+) also suppressed activity, and the veratridine effect was completely reversed by tetrodotoxin or by omission of Ca(2+) when [gamma-(32)P]ATP was used, and partially reversed when (32)PO(4) (2-) was used. We conclude that the morphological transformation of an axon into a terminal is accompanied by significant changes in protein and phospho-protein composition that may be related to synaptic transmission.
mesh_terms: D000818:Animals; D001369:Axons / Q000378:metabolism*; D002118:Calcium / Q000494:pharmacology; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone / Q000494:pharmacology; D003599:Cytoskeleton / Q000378:metabolism; D049832:Decapodiformes; D008564:Membrane Potentials; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D011494:Protein Kinases / Q000378:metabolism; D013574:Synaptosomes / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D009419:Nerve Tissue Proteins; D010750:Phosphoproteins; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone; D011494:Protein Kinases; D002118:Calcium