title: Stimulation by human placental conditioned medium of hemopoietic colony formation by human marrow cells.
abstract: Medium conditioned by human placental tissue was found to stimulate granulocytic and monocytic colony formation by human marrow cells in semisolid agar cultures. The colony-stimulating activity of unfractionated conditioned medium was equivalent to the activity of standard peripheral blood underlayers. Placentas were a reliable source of active material, and one placenta provided enough material to stimulate 5,000-10,000 cultures of normal or leukemic cells. The colony-stimulating factor in human placental conditioned medium (CSFHPCM) was concentrated and purified 1800-fold using ammonium sulfate precipitation, calcium phosphate gel absorption, DEAE-cellulose batch absorption, gel filtration on Sephadex G-150, and polyarcylamide gel gel electrophoresis. The active factor behaved on gel filtration as a macromolecule with an apparent molecular weight of 30,000 daltons. The active factor in placental conditioned medium was not dependent on the presence of adherent marrow cells with endogenous colony-stimulating activity.
mesh_terms: D001853:Bone Marrow; D001854:Bone Marrow Cells; D002455:Cell Division; D003115:Colony-Stimulating Factors / Q000302:isolation & purification; D003470:Culture Media; D046508:Culture Techniques; D006023:Glycoproteins / Q000302:isolation & purification; D006098:Granulocytes; D006801:Humans; D009000:Monocytes; D010920:Placenta
chemical_list: D003115:Colony-Stimulating Factors; D003470:Culture Media; D006023:Glycoproteins