title: Site-specific initiation of a DNA fragment: nucleotide sequence of the bacteriophage G4 negative-strand initiation site.
abstract: The synthesis of the bacteriophage G4 negative strand is an example of the de novo initiation of a polynucleotide chain. This initiation is performed by the Escherichia coli replication protein dna G which selects a unique site on 5400-base positive-strand template. In this paper we present the nucleotide sequence of the G4 negative-strand initiation site. This is the template element recognized by the dna G priming protein. In conjunction with the sequence of the nascent negative strand, obtained by Bouché, Rowen, and Kornberg [Bouché, J.-P., Rowen, L. & Kornberg, A. (1978) J. Biol. Chem. 253, 765-769], the present data provide a description of a dna G-dependent origin of replication in which one knows the place at which polymerization starts at the nucleotide level.
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping; D003090:Coliphages / Q000235:genetics*; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D004277:DNA, Single-Stranded / Q000378:metabolism*; D004279:DNA, Viral / Q000096:biosynthesis*; D005814:Genes, Viral; D009690:Nucleic Acid Conformation; D014779:Virus Replication
chemical_list: D004277:DNA, Single-Stranded; D004279:DNA, Viral; D004262:DNA Restriction Enzymes