Stage-sensitivity and dose-response study after gamma-irradiation of mouse primary spermatocytes.
The sensitivity pattern for X-ray-induced chromatid aberrations was analysed in primary spermatocytes irradiated at 1 to 11 days diakinesis-metaphase I of meiosis. Using a dose of 300 R (60 R/min) of gamma-rays zygotene (day 9) was found to be the most sensitive and leptotene (day 11) the most insensitive stage. The dose-response to gamma-ray-induced aberrations was evaluated in cells irradiated zygotene. Both the yields of rearrangement as well as the yields of fragments gave a best fit to a quadratic model.
D000818:Animals; D002842:Chromatids / Q000528:radiation effects; D002869:Chromosome Aberrations; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D006824:Hybridization, Genetic; D007621:Karyotyping; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D011839:Radiation, Ionizing; D013090:Spermatocytes / Q000528:radiation effects*; D013094:Spermatozoa / Q000528:radiation effects*