Novel idiotypic and antigen-binding characteristics in two anti-dinitrophenyl monoclonal antibodies.
Monoclonal anti-dinitrophenyl antibodies generated in the splenic focus system from B cells of adult BALB/c mice were studied for the presence or absence of murine anti-T15 (M anti-T15) reactivity and for their ability to bind phosphorylcholine (PC). Two foci of the 680 clones analyzed bound PC, and one of these antibodies reacted with M anti-T15 and anti-Fab on a 1:1 weight basis. The discovery of a clonotype reactive with M anti-15 but not with rabbit anti-T15 (R anti-T15) serum, the converse of the R anti-T15+, M anti-T15- clonotype identified in the PC-specific repertoire, points to the novel idiotypic relationships which may be found among homogeneous antibodies binding diverse antigens. The R anti-T15-, M anti-T15+ clonotype may represent a distinct set of hypervariable region sequences inserted into the T15 framework or may be a somatic variant of the T15 germ-line sequence. In addition, the maximum frequency with which this clonotype occurs within the B-cell pool is estimated.
D000818:Animals; D000906:Antibodies; D000937:Antigen-Antibody Reactions; D000951:Antigens, Neoplasm; D001402:B-Lymphocytes / Q000276:immunology; D001666:Binding Sites, Antibody; D004140:Dinitrophenols / Q000276:immunology*; D005838:Genotype; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology
D000906:Antibodies; D000951:Antigens, Neoplasm; D004140:Dinitrophenols