Studies on the product of antigenic recognition. III. Nature of PAR.
The product of antigenic recognition (PAR) is an antigen-antibody complex with granulotactic activity. The following results support his conclusion. PAR generated by cell-bound or shed T-cell RS or the recognizing structure of T alloantisera and alloantigen can be destroyed by heating at 56 degrees C for 30 min, because of inactivation of a heat-labile component supplied by alloantigen. Recognizing partners of these complexes are heat-stable. They form PAR anew when alloantigen is added or upon confrontation with anti-RS serum. They are also capable of inducing anti-RS sera. PAR preparations composed of these recognizing structures and antibodies to them are heat-inactivated at much higher temperatures, because both partners of the complex are relatively heat-stable. Heat-labile PAR complexes (recognizing structures and alloantigen) pass anti-mouse immunoglobulin immunosorbent, but are retained specifically by alloantiserum (via alloantigen of the complex) or by anti-RS serum (via RS of the complex) bound to the column.
D000818:Animals; D000936:Antigen-Antibody Complex; D001666:Binding Sites, Antibody; D006224:Cricetinae; D005260:Female; D006358:Hot Temperature; D007106:Immune Sera; D007156:Immunologic Memory; D007518:Isoantibodies; D007519:Isoantigens; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D014181:Transplantation Immunology
D000936:Antigen-Antibody Complex; D007106:Immune Sera; D007518:Isoantibodies; D007519:Isoantigens