GLC determination of indoprofen in plasma.
Implementation of human bioavailability studies with the analgesic indoprofen required a rapid, sensitive, and convenient assay in blood plasma. A procedure based on ether extraction of acidified plasma, derivatization with diazomethane, and GLC analysis using a 3% OV-1 column was sufficiently sensitive for measurement of plasma indoprofen concentrations in the 0.4-16 mug/ml range. An average recovery of 99.0 +/- 7.6% (SD) was achieved when the pentanoic acid homolog was employed as an internal standard.
D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000097:blood*; D002849:Chromatography, Gas; D003978:Diazomethane; D006801:Humans; D007211:Indoles / Q000097:blood; D010666:Phenylpropionates / Q000097:blood*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D007211:Indoles; D010666:Phenylpropionates; D003978:Diazomethane