2 micrometer covalently closed non-mitochondrial circular DNA in the petite-negative yeast Schizosaccharomyces pombe.
A population of small covalently closed non-mitochondrial circular DNA molecules was isolated from the petite-negative yeast Schizosaccharomyces pombe. The mean length of these molecules, possessing the same density as nuclear DNA (1.695 g/cm3) is 1.95 +/- 0.18 micrometer. The presence of these minicircles in crude mitochondrial preparations indicates their tight association with mitochondrial particles. Their disappearance after DNase treatment of mitochondria demonstrates their extramitochondrial location.
D001203:Ascomycota / Q000235:genetics*; D002458:Cell Fractionation; D002499:Centrifugation, Density Gradient; D004270:DNA, Circular / Q000302:isolation & purification*; D008854:Microscopy, Electron; D008928:Mitochondria; D009690:Nucleic Acid Conformation; D010641:Phenotype; D012568:Schizosaccharomyces / Q000235:genetics*
D004270:DNA, Circular