Use of hamster hepatocytes to metabolize carcinogens in an in vitro bioassay.
Adult Syrian golden hamster hepatocytes were shown to have a high capacity to metabolize carcinogens, such as diethylnitrosamine, 2-nitrofluorene, and 4-aminoazobenzene, to a form that transforms mammalian cells. The addition of hepatocytes to the hamster embryo cell transformation system extends its range of usefulness for detecting carcinogens that require metabolic activation.
D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000378:metabolism; D001711:Biotransformation; D002273:Carcinogens / Q000378:metabolism*; D002471:Cell Transformation, Neoplastic; D002999:Clone Cells; D006224:Cricetinae; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D005260:Female; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism*; D008297:Male; D008647:Mesocricetus
D002273:Carcinogens; D001189:Aryl Hydrocarbon Hydroxylases