Possible effects of non-HLA antibodies in common typing sera on HLA antigen frequency data in leukemia.
Selective adsorption of several common "monospecific" HLA typing sera with HLA typed platelets, purified B lymphocytes, cultured lymphoid cells, or lymphocytes from patients with active chronic lymphocytic leukemia demonstrated that many of these sera contain antibodies to non-HLA antigens. Antibodies were detected to antigens present on peripheral blood B-lymphocytes, cultured lymphoid cells and leukemic cells from patients with both myelocytic and lymphocytic forms of leukemia but absent from T-lymphocytes and platelets. Since these kinds of antibodies appear to be present in a large proportion of common HLA typing sera, caution should be used in interpreting all data related to HLA antigen expression in leukemia.
D000906:Antibodies / Q000032:analysis*; D001402:B-Lymphocytes / Q000276:immunology; D001792:Blood Platelets / Q000276:immunology; D006680:HLA Antigens / Q000032:analysis*; D006649:Histocompatibility Antigens / Q000032:analysis*; D006650:Histocompatibility Testing; D006801:Humans; D007106:Immune Sera / Q000032:analysis; D007938:Leukemia / Q000276:immunology*; D007945:Leukemia, Lymphoid / Q000276:immunology
D000906:Antibodies; D006680:HLA Antigens; D006649:Histocompatibility Antigens; D007106:Immune Sera