title: Molecular properties of T lymphoma immunoglobulin. I. Serological and general physicochemical properties.
abstract: Immunoglobulin (Ig) released into the medium by monoclonal continuously cultured murine T lymphoma cells of the lines WEHI-22 and WEHI-7 was isolated by serological precipitation or solid-phase immunoadsorption techniques. The intact immunoglobulin had an electrophoretic mobility on sodium dodecyl-sulphate (SDS) containing polyacrylamide gels comparable to that of IgG (mass 150,000). This mobility was significantly faster than that of '7S' IgM of murine B lymphocyte surfaces. The T lymphoma immunoglobulin consisted of a pair of heavy chains linked by disulphide bonds and light chains non-covalently bound to the heavy chains. The isolated heavy chains migrated slightly faster than the mu chains of MOPC 104E IgM. Some, but not all, antisera directed against mu chains of normal mouse serum IgM bound T lymphoma immunoglobulin apparently via a cross-reaction localized to the Fd fragment. These data indicate that immunoglobulin of T lymphoma cells and, presumably normal T lymphocytes, represents an immunoglobulin isotype which is distinct from those immunoglobulins found on the B cell surface.
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D055598:Chemical Phenomena; D002621:Chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D007106:Immune Sera / Q000494:pharmacology; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins; D008223:Lymphoma / Q000276:immunology*; D051379:Mice; D008968:Molecular Conformation; D011817:Rabbits; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D007106:Immune Sera; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins