Cell-mediated immunity to tumor antigen in Marek's disease: susceptibility of effector cells to antithymocyte serum and enhancement of cytotoxic activity by Vibrio cholerae neuraminidase.
Spleen cells from chickens inoculated 7 to 8 days previously with Marek's disease virus were cytotoxic for 51Cr-labeled cells of a Marek's disease lymphoblastoid cell line (MSB-1 line) in a 4-h in vitro cytotoxic assay. The cytotoxic activity of spleen cells was inhibited by pretreatment with antithymocyte serum and complement, but not with complement alone or in combination with anti-bursa cell serum or normal preimmune serum. The conclusion was that the effector cell in the above cytotoxic assay was a thymus-derived lymphocyte. Also, pretreatment of target cells with Vibrio cholerae neuraminidase enhanced in vitro cytotoxic activity of effector cells. Similar enzymatic treatment of effector cells had a negligible effect on cytotoxicity.
D000818:Animals; D000951:Antigens, Neoplasm; D000961:Antilymphocyte Serum; D002645:Chickens; D003602:Cytotoxicity, Immunologic; D007111:Immunity, Cellular; D008380:Marek Disease / Q000276:immunology*; D009439:Neuraminidase / Q000378:metabolism; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014734:Vibrio cholerae / Q000201:enzymology
D000951:Antigens, Neoplasm; D000961:Antilymphocyte Serum; D009439:Neuraminidase