A gas chromatographic method for the evaluation of the vinyl epoxidase activity.
Two alternative specific and very sensitive methods for determination of vinyl epoxide synthetase activity in liver microsomes are described. Trichloroethylene, which is used as a substrate, is converted into trichloroethylene oxide by a hepatic epoxide synthetase. Chloral hydrate, the final rearrangement product of trichloroethylene oxide, is evaluated by electron capture gas chromatography, either after derivatization with pentafluorophenyl-hydrazine or after its conversion into chloroform under alcaline conditions. The kinetic parameters of the epoxidative reaction have been determined on rat hepatic microsomal suspensions.
D000818:Animals; D002697:Chloral Hydrate / Q000032:analysis; D002725:Chloroform / Q000032:analysis; D002849:Chromatography, Gas; D004852:Epoxy Compounds; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D008722:Methods; D008862:Microsomes, Liver / Q000201:enzymology; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism*; D051381:Rats; D014753:Vinyl Compounds / Q000378:metabolism*
D004852:Epoxy Compounds; D014753:Vinyl Compounds; D002697:Chloral Hydrate; D002725:Chloroform; D006899:Mixed Function Oxygenases; D010088:Oxidoreductases