title: Studies on production of biologically active substance which inhibits the intracellular multiplication of Toxoplasma within mouse macrophages.
abstract: In vitro assessments were carried out to study some biological aspects of immune lymphocytes producing a new lymphokine, called by the authors Toxoplasma growth inhibitory factor (Toxo-GIF), which inhibits the intracellular multiplication of Toxoplasma gondii within nonimmune mouse macrophages. Concanavalin A and phytohemagglutinin-P were found to induce vigorous production of Toxo-GIF, whereas bacterial lipopolysaccharide did not. In vitro treatment of splenic lymphocytes with rabbit anti-mouse thymocyte serum plus complement abolished almost completely their ability to produce Toxo-GIF. Treatment of splenic lymphocytes with inhibitors of protein synthesis, cycloheximide or puromycin resulted in a remarkable reduction of the ability of sensitized lymphocytes to produce this lymphokine. Thus the production of Toxo-GIF seems to be dependent on the cellular metabolic events of sensitized T-lymphocytes. The significant activity of Toxo-GIF was demonstrable even in the supernate of lymphocyte cultures incubated in serum-free medium and was also evident after immune lymphocytes and homologous antigen were incubated for the relatively short period of 10 h.
mesh_terms: D000818:Animals; D000961:Antilymphocyte Serum / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D037102:Lectins / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D008222:Lymphokines / Q000096:biosynthesis* / Q000494:pharmacology; D008264:Macrophages / Q000469:parasitology*; D008297:Male; D051379:Mice; D011135:Polysaccharides, Bacterial / Q000494:pharmacology; D011691:Puromycin / Q000494:pharmacology; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology; D014122:Toxoplasma / Q000187:drug effects / Q000254:growth & development*; D014123:Toxoplasmosis / Q000097:blood*
chemical_list: D000961:Antilymphocyte Serum; D037102:Lectins; D008070:Lipopolysaccharides; D008222:Lymphokines; D011135:Polysaccharides, Bacterial; D003208:Concanavalin A; D011691:Puromycin; D003513:Cycloheximide