title: [Effects of UV-C and of 8-MOP + UV-A on the T- and B-population of human lymphocytes in vitro (author's transl)].
abstract: The transformation of UV-C irradiated leucocytes and lymphocytes by the mitogens Con A, PHA and PWM is measured by the 3H-Tdr. incorporation after 72 h incubation. Furthermore T- and B-cells are determined by the method of rosette formation. A clear inhibition of the cell activity is seen after irradiation of leucocytes with 450 mJ/cm(2) and of a lymphocyte suspension with 5-10 mJ/cm(2). There are no significant differences between the effects of the various mitogens. The number of T-cells decreases proportionality to the various intensity, the number of B-cells remain constant. Irradiation with UV-A + 8-MOP cause, equally for all mitogens, a dosis dependent inhibition of thymidine incorporation.
mesh_terms: D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000528:radiation effects; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects / Q000528:radiation effects; D008730:Methoxsalen / Q000494:pharmacology*; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D008730:Methoxsalen
vernacular_title: Wirkung von UV-C und von 8-MOP + UV-A auf die T- und B-Population menschlicher Lymphocyten in vitro.