Topographic study of the cell surface of micrococcus radiodurans.
The paracrystalline outer membraneous layer (HPI layer) of Micrococcus radiodurans has been investigated by negative- and positive-staining electron microscopy and subsequent digital image processing. The subunit structure of the major HPI layer protein complex and the lipid-protein distribution in the plane of the membrane have been determined. The HPI layer was found to be highly asymmetric in a transmembrane direction, with the major protein complex only partly penetrating into a lipid-containing backing layer intimately associated with it.
D001426:Bacterial Proteins / Q000032:analysis; D002462:Cell Membrane / Q000648:ultrastructure*; D008563:Membrane Lipids / Q000032:analysis; D008565:Membrane Proteins / Q000032:analysis; D008837:Micrococcus / Q000648:ultrastructure*; D008854:Microscopy, Electron
D001426:Bacterial Proteins; D008563:Membrane Lipids; D008565:Membrane Proteins