A human haemic cell line capable of cellular and humoral killing of normal and malignant cells.
A line of human leukaemia-derived cells is described that kills a wide range of human and animal cell lines, whether normal or malignant, even at a ratio of 1:1. During exposure to the target cells, the killer cells released a factor into the culture medium which destroyed target cells in the absence of the killer cells. This phenomenon occurs without exogenous complement and requires no pre-treatment of target or killer cells. The humoral factor is a protein precipitable by 60% saturation of ammonium sulphate and has a mol. wt. of approximately 70,000; It prevented the growth of a fibrosarcoma in mice.
D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D003601:Cytotoxicity Tests, Immunologic; D005354:Fibrosarcoma / Q000276:immunology; D006801:Humans; D007938:Leukemia / Q000276:immunology* / Q000648:ultrastructure; D007962:Leukocytes / Q000276:immunology*; D008233:Lymphotoxin-alpha; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D012513:Sarcoma, Experimental / Q000276:immunology; D013997:Time Factors
D008233:Lymphotoxin-alpha