Role of T lymphocytes in the humoral immune response III. Lack of influence of antigen charge on the isotype and charge of antibody.
The influence of antigen charge on the anti-hapten antibody response and antigen-stimulated blastogenic response in vitro was measured in guinea pigs. The animals were immunized with dinitrophenylated bovine albumin (DNP-BSA; pl 4.9), methylated DNP-BSA (DNP-MBSA; pl greater than 10), and DNP-lysozyme (pl greater than 10). While the lymph node lymphocytes clearly distinguished between DNP-BSa and DNP-MBSA in terms of the magnitude of the antigen-stimulated 3H-thymidine incorporation, no differences were seen with respect to the characteristics of the antibody response. Thus, IgG1-to-IgG2 ratios, DEAE cellulose fractions and isoelectric focus profiles of anti-DNP sera from animals immunized with DNP-BSA, DNP-MBSA and DNP-lysozymes were indistinguishable.
D000818:Animals; D000917:Antibody Formation; D004136:Dinitrobenzenes; D006168:Guinea Pigs; D007126:Immunoglobulin Allotypes; D007074:Immunoglobulin G / Q000096:biosynthesis; D066298:In Vitro Techniques; D007525:Isoelectric Focusing; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000378:metabolism; D009113:Muramidase; D012710:Serum Albumin, Bovine; D013601:T-Lymphocytes / Q000276:immunology*; D013936:Thymidine / Q000378:metabolism
D004136:Dinitrobenzenes; D007126:Immunoglobulin Allotypes; D007074:Immunoglobulin G; D012710:Serum Albumin, Bovine; D009113:Muramidase; D013936:Thymidine