Bimane fluorescent labels: labeling of normal human red cells under physiological conditions.
The bimane fluorescent labels, monobromobimane, dibromobimane, and monobromotrimethylammoniobimane, are derivatives of syn-9,10-dioxabimane:1,5-diazabicyclo[3.3.0]octa-3,6-diene-2,8-dione. They efficiently label hemoglobin (reactive thiol groups), membrane proteins, and glutathione of normal human red cells under physiological conditions. Monobromobimane and dibromobimane are effective on intact cells while red cell membranes may be impermeable to the positively charged monobromotrimethylammoniobimane, the latter being effective only on lysed cells. These bimane labels provide a class of labeling agents that may have wide applicability in biological materials.
D001372:Aza Compounds / Q000378:metabolism; D001798:Blood Proteins / Q000378:metabolism; D001643:Bridged Bicyclo Compounds / Q000378:metabolism*; D001952:Bridged-Ring Compounds / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000378:metabolism*; D005914:Globins / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008563:Membrane Lipids / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D013347:Subcellular Fractions / Q000378:metabolism
D001372:Aza Compounds; D001798:Blood Proteins; D001643:Bridged Bicyclo Compounds; D001952:Bridged-Ring Compounds; D005456:Fluorescent Dyes; D008563:Membrane Lipids; D008565:Membrane Proteins; D005914:Globins; D005978:Glutathione