Aspects of the reversal of methotrexate toxicity in rodents.
An ip injection of a Lactobacillus casei dihydrofolate reductase preparation into rats and mice given a single lethal dose of methotrexate (MTX) caused a marked lowering of free MTX in the blood. Alternatives to citrovorum factor as agents for reversing MTX toxicity were explored in mice. dl, L-5-Methyltetrahydrofolate, dl,L-5,10-methylenetetrahydrofolate, l,L-5,10-methylenetetrahydrofolate, and dihydrofolate were also effective MTX antagonists; d,L-5,10-methylenetetrahydrofolate was inert.
D000818:Animals; D005492:Folic Acid / Q000031:analogs & derivatives / Q000627:therapeutic use; D002955:Leucovorin / Q000627:therapeutic use; D008297:Male; D008727:Methotrexate / Q000097:blood / Q000633:toxicity*; D051379:Mice; D051381:Rats; D013762:Tetrahydrofolate Dehydrogenase / Q000494:pharmacology / Q000627:therapeutic use; D013763:Tetrahydrofolates / Q000627:therapeutic use
D013763:Tetrahydrofolates; D005492:Folic Acid; D013762:Tetrahydrofolate Dehydrogenase; D002955:Leucovorin; D008727:Methotrexate