Detection and characterization of DNA-anti-DNA complexes in a patient with systemic lupus erythematosus.
DNase digestion of SLE serum, with consequent release of bound DNA antibody has been proposed as a method for the direct demonstration of circulating DNA-anti-DNA complexes. In the present studies on the serum of a girl with active SLE nephritis, circulating DNA-anti-DNA complexes were demonstrated at the precise time of relapse of SLE nephritis. Ultracentrifugation showed that these complexes were of low molecular weight.
D000974:Antibodies, Antinuclear / Q000032:analysis*; D000936:Antigen-Antibody Complex; D001666:Binding Sites, Antibody; D002648:Child; D004247:DNA / Q000276:immunology*; D003851:Deoxyribonucleases / Q000494:pharmacology; D005260:Female; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*
D000974:Antibodies, Antinuclear; D000936:Antigen-Antibody Complex; D004247:DNA; D003851:Deoxyribonucleases