Chemical synthesis of genes for human insulin.
A rapid chemical procedure has been developed and used for the synthesis of 29 oligodeoxyribonucleotides to build synthetic genes for human insulin. The gene for insulin B chain, 104 base pairs, and the one for A chain, 77 base pairs, were designed from the amino acid sequence of human polypeptides. They bear single-stranded cohesive termini for the EcoRI and BamHI restriction endonucleases and are designed to be inserted separately into a pBR322 plasmid. The synthetic fragments, deca- to pentadecanucleotides, were synthesized by a block phosphotriester method with trinucleotides as building blocks. Final purification was by high-performance liquid chromatography. All 29 oligonucleotides were pure and had the correct sequences.
D004247:DNA / Q000138:chemical synthesis; D004274:DNA, Recombinant; D005796:Genes; D006801:Humans; D007328:Insulin / Q000235:genetics*; D008722:Methods; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000302:isolation & purification; D009841:Oligonucleotides / Q000138:chemical synthesis*
D004274:DNA, Recombinant; D007328:Insulin; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D004247:DNA