Cell surface labelling of mononuclear cells with antisera associated to turnip yellow mosaic virus of alphalpha mosaic virus particles. A freeze-etch study.
Turnip yellow mosaic virus (TYMV) and alphalpha mosaic virus (AMV) were used as immuno-electron microscopical markers to detect cell surface receptors on mononuclear cells in freeze-etch replicas. TYMV particles were conjugated with vacuum-distilled glutaraldehyde to rabbit IgG anti-mouse immunoglobulins (TYMV-RAMIg conjugate) or to rabbit IgG anti-mouse theta antigen (TYMV-RAMTh conjugate). B-lymphocytes incubated with TYMV-RAMIg conjugate showed either randomly distributed particles or patches of virus particles on the etched surface of the cell membrane. Mouse thymocytes incubated with TYMV-RAMTh conjugate, however, showed only a random distribution of the virus particles. Human mononuclear cells incubated with rabbit IgG anti-AMV and AMV for the demonstration of the receptors for the Fc fragment of IgG showed the oblong shape of the AMV particles on the etched cell membrane, Fc receptors were either randomly distributed or aggregrated into patches. It is concluded that both types of virus particles are useful markers for the demonstration of membrane receptors in freeze-etch replicas of labelled cells.
D000818:Animals; D001402:B-Lymphocytes / Q000032:analysis / Q000648:ultrastructure; D001665:Binding Sites; D002462:Cell Membrane / Q000032:analysis* / Q000276:immunology / Q000648:ultrastructure; D005613:Freeze Etching / Q000379:methods; D008198:Lymph Nodes / Q000032:analysis / Q000276:immunology / Q000648:ultrastructure; D051379:Mice; D008819:Mice, Nude; D008854:Microscopy, Electron / Q000379:methods; D009029:Mosaic Viruses; D010942:Plant Viruses; D011817:Rabbits / Q000276:immunology; D013950:Thymus Gland / Q000032:analysis / Q000276:immunology / Q000648:ultrastructure