Embryo transport through the mare's oviduct depends upon cleavage and is independent of the ipsilateral corpus luteum.
Two experiments were conducted using 14 mares. In Exp. 1, mares were inseminated with semen treated with TEPA, which, in other species, has been shown to lead to an arrest in ovum cleavage at 2--4 cells. The oviducts and/or uterus were then flushed 7--10 days after ovulation in 6 mares (Group A) or 2--6 days after ovulation in 5 mares (Group B). Fresh eggs were found in the oviduct flushes of 5 Group A  and 5 Group B mares: 9 of the 10 eggs appeared to have cleaved, but none had developed beyond 16-cells. Seven eggs contained spermatozoa and 3 of 4 eggs from each group showed evidence of fertilization when examined ultrastructurally. Group A mares had thus retained fertilized eggs in the oviduct beyond the time at which they would normally have entered the uterus (6 days), indicating that development beyond at least the 2- to 4-cell stage is necessary for normal transport. In Exp. 2, 5 attempts were made to recover the embryo within 4 days of ovulation and transfer it to the contralateral oviduct. A single pregnancy resulted, indicating that a unilateral interaction with the corpus luteum was not necessary for the transport of the embryo to the uterus.
D000818:Animals; D002970:Cleavage Stage, Ovum / Q000502:physiology; D003338:Corpus Luteum / Q000502:physiology; D004624:Embryo Transfer / Q000379:methods / Q000662:veterinary*; D005187:Fallopian Tubes / Q000502:physiology*; D005260:Female; D005306:Fertilization; D006736:Horses / Q000502:physiology*; D010060:Ovulation; D011247:Pregnancy; D011270:Pregnancy, Animal