Introduction and expression of a rabbit beta-globin gene in mouse fibroblasts.
The cloned chromosomal rabbit beta-globin gene has been introduced into mouse fibroblasts by DNA-mediated gene transfer (transformation). In this report, we examine the expression of the rabbit gene in six independent transformants that contain from 1 to 20 copies of the cloned globin gene. Rabbit globin transcripts were detected in two of these transformants at steady-state concentrations of 5 and 2 copies per cell. The globin transcripts from one cell line are polyadenylylated and migrate as 9S RNA on methylmercury gels. These transcripts reflect correct processing of the two intervening sequences but lack 48 +/- 5 nucleotides present at the 5' terminus of rabbit erythrocyte globin mRNA.
D000818:Animals; D001483:Base Sequence; D004274:DNA, Recombinant; D005347:Fibroblasts; D005796:Genes; D005914:Globins / Q000235:genetics*; D051379:Mice; D008970:Molecular Weight; D009694:Nucleic Acid Precursors / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D011817:Rabbits; D013045:Species Specificity; D014158:Transcription, Genetic; D014170:Transformation, Genetic
D004274:DNA, Recombinant; D009694:Nucleic Acid Precursors; D012333:RNA, Messenger; D005914:Globins