Isolation and characterization of a tumor cell surface antigen from spontaneously transformed BALB/c mouse fibroblasts.
This work describes the detection, isolation, and partial characterization of a BALB/c mouse fibroblast cell surface antigen. This antigen migrates as a polypeptide of approximately 100,000 daltons in a discontinuous sodium dodecyl sulfate/polyacrylamide gel electrophoresis system, can be labeled by either lactoperoxidase-catalyzed cell surface 125I iodination or metabolic incorporation of [3H]glucosamine, and can be isolated by concanavalin A affinity chromatography. This cell surface glycoprotein is antigenic in BALB/c mice and has been correlated with the rejection of immunogenic tumor cells. Also, antiserum specific for Moloney leukemia virus precipitates the 100,000-dalton cell surface protein from viral and immunogenic spontaneous transformants. This virus-related antigen comigrates on sodium dodecyl sulfate gels with the major iodinated cell surface protein of these transformants. Rabbit antiserum to the purified antigen demonstrates a marked preference for the surfaces of immunogenic tumor cells as compared with normal cells and nonimmunogenic tumor cells.
D000818:Animals; D000951:Antigens, Neoplasm / Q000302:isolation & purification*; D000954:Antigens, Surface / Q000302:isolation & purification*; D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000032:analysis*; D003429:Cross Reactions; D006023:Glycoproteins / Q000276:immunology* / Q000302:isolation & purification; D051379:Mice; D008807:Mice, Inbred BALB C; D008970:Molecular Weight; D008979:Moloney murine leukemia virus / Q000276:immunology; D009374:Neoplasms, Experimental / Q000276:immunology*
D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D000956:Antigens, Viral; D006023:Glycoproteins