Chemical carcinogens produce mutations to ouabain resistance in transformable C3H/10T1/2 Cl 8 mouse fibroblasts.
Chemical carcinogens induce mutations to ouabain resistance in the transformable mouse fibroblast cell line C3H/10T1/2 Cl 8. The mutant phenotype is stable and heritable in the absence of selective agent, and dose--response curves for mutant frequency were obtained with N-menthyl-N'-nitro-N-nitrosoguanidine, 3-methylcholanthrene, benzo[a]-pyrene, N-acetoxy-N-2-acetylaminofluorene, and the anti-7,8-diol-9,10-epoxide of benzo[a]pyrene. The ratio of the malignant transformation frequency to the mutation frequency was 12 for benzo[a]pyrene and 21 for N-acetoxy-N-2-acetylaminofluorene. The development of the mutational assay reported here allows the use of this permanent cell line for comparison of mutation and transfomration frequencies and as a screening system for xenobiotics that pose mutagenic or carcinogenic hazards to mammalian cells.
D015073:2-Acetylaminofluorene / Q000494:pharmacology; D000818:Animals; D002273:Carcinogens / Q000494:pharmacology; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004351:Drug Resistance; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D051379:Mice; D008809:Mice, Inbred C3H; D009154:Mutation / Q000187:drug effects*; D010042:Ouabain / Q000494:pharmacology; D013997:Time Factors
D002273:Carcinogens; D008769:Methylnitronitrosoguanidine; D010042:Ouabain; D015073:2-Acetylaminofluorene