Effects of 60Co gamma-irradiation on chondromucoprotein.
Pulse radiolysis and 60Co gamma-radiolysis have been used to study the effects of ionizing radiations on the protein-polysaccharide complex, chondromucoprotein. Both hydroxyl radicals and hydrated electrons readily react with the complex, with second-order rate-constants of 4-55 X 10(8)M-1s-1, respectively. Depolymerization of the carbohydrate component, which is initiated by reaction of OH, readily occurs after irradiation in aqueous solution, as is evidenced by an increase in the formation of reducing-end groups and a decrease in the viscosity of the solution. The ability of the anionic carbohydrate component to bind cationic counter-ions also decreases.
D000818:Animals; D002417:Cattle; D002807:Chondroitin / Q000031:analogs & derivatives*; D003037:Cobalt Radioisotopes; D005609:Free Radicals; D005720:Gamma Rays; D007700:Kinetics; D009088:Mucoproteins / Q000528:radiation effects*; D011839:Radiation, Ionizing; D012996:Solutions; D014783:Viscosity
D003037:Cobalt Radioisotopes; D005609:Free Radicals; D009088:Mucoproteins; D012996:Solutions; D002807:Chondroitin