3H-glucosamine electron microscope autoradiography after isolated labeling of the enamel organ or the dental papilla followed by reassociated toothgerm culture.
Electron microscope autoradiography was performed after isolated labeling with 3H-glucosamine of the enamel organ or the dental papilla of Swiss mice followed by reassociated toothgerm culture for 9.30 h and 19 h. After these two time intervals in case of an enamel organ labeling, numerous silver grains were observed in the intercellular spaces of the stellate reticulum indicating a concentrating and storing role of the latter. After dental papilla labeling, an almost complete absence of radioactivity was noted at 19 h. suggesting that 3H-glucosamine is not stored by the dental papilla. A clear labeling of the basement membrane area and of the adjacent aperiodic filamentous material found on the pulpal side was only observed after enamel organ labeling with 3H-glucosamine, suggesting an epithelial origin of the glycosaminoglycans found in these structures.
D000818:Animals; D001345:Autoradiography / Q000379:methods; D001485:Basement Membrane / Q000378:metabolism; D046508:Culture Techniques / Q000379:methods*; D003771:Dental Papilla / Q000378:metabolism* / Q000648:ultrastructure; D004658:Enamel Organ / Q000378:metabolism* / Q000648:ultrastructure; D005944:Glucosamine / Q000378:metabolism*; D006025:Glycosaminoglycans / Q000378:metabolism; D051379:Mice; D008854:Microscopy, Electron / Q000379:methods; D009805:Odontogenesis; D014083:Tooth Germ / Q000378:metabolism*; D014316:Tritium
D006025:Glycosaminoglycans; D014316:Tritium; D005944:Glucosamine