title: Nuclear RNA content and synthesis in anterior pituitary in intact, castrated and androgen sterilized rats.
abstract: The content and uridine incorporation into nuclear RNA of anterior pituitary gland was studied in vitro in intact, castrated and neonatally testosterone treated rats of both sexes. In female rats a higher RNA content and a lower uridine incorporation rate could be observed than in males. The castration resulted in a marked increase of RNA content and uridine incorporation in male rats. Ovariectomy was followed by a decrease of RNA content which was accompanied by an increase of incorporation rate. Estradiol administration produced a biphasic change of RNA content and uridine incorporation in ovariectomized rats. From these findings a repressive action of estrogens on the nuclear RNA synthesis may be suggested. Neonatal testosterone treatment resulted in alterations of RNA synthesis which could be observed in both sexes.
mesh_terms: D000818:Animals; D002369:Castration; D002467:Cell Nucleus / Q000378:metabolism*; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D008297:Male; D010903:Pituitary Gland, Anterior / Q000378:metabolism*; D012313:RNA / Q000096:biosynthesis*; D051381:Rats; D013739:Testosterone / Q000494:pharmacology*; D014529:Uridine / Q000378:metabolism
chemical_list: D013739:Testosterone; D004958:Estradiol; D012313:RNA; D014529:Uridine