title: Genetic engineering.
abstract: The field of genetic engineering is reviewed with a special emphasis on in vitro DNA recombinant technology. The basic principles of the biochemistry of DNA splicing and of gene transfer are described. An important distinction is made between the insertion and cloning of genes derived from genomic DNA ("natural" genes) and of DNA synthetized in vitro ("synthetic" genes). Cloning of genes synthetized from mRNA has provided the probes necessary for the identification of genomic clones, and recently it has made possible the synthesis of specific mammalian proteins in bacteria.
mesh_terms: D004274:DNA, Recombinant; D005818:Genetic Engineering; D005827:Genetics, Microbial; D066298:In Vitro Techniques; D014176:Protein Biosynthesis; D012333:RNA, Messenger
chemical_list: D004274:DNA, Recombinant; D012333:RNA, Messenger