Surface antigen changes accompanying lymphocyte activation. Selective reactivity of certain anti-H-2 antisera with activated lymphocytes of unrelated H-2 haplotype.
It is here shown that some noncongenic anti-H-2 antisera produced by using normal spleen cells at the immunogen can contain cytotoxic antibody activity against activated, but not resting, lymphocytes of unrelated H-2 haplotype strains. Reactivity was noted against lymphocytes activated in vitro with T- or B-cell mitogens, or in vivo by antigenic stimulation. Analysis of the results indicated that the anti-activated lymphocyte activity was most likely related to production of anti-Ala-1 ('activated lymphocyte antigen') alloantibodies. This appears to occur as a result of donor lymphocyte activation during production of the anti-H-2 antisera, using Ala-1-incompatible donor and recipient combinations, where the donor cells are competent to recognize and react against host H-2 incompatibilities. These results confirm the finding that lymphocyte activation can be accompanied by the expression of an antigen that is either absent or weakly expressed on resting lymphocytes.
D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity; D000941:Antigens; D001402:B-Lymphocytes / Q000276:immunology; D002462:Cell Membrane / Q000276:immunology*; D006183:H-2 Antigens; D006238:Haploidy; D007518:Isoantibodies; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008934:Mitogens / Q000494:pharmacology; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000276:immunology
D000941:Antigens; D006183:H-2 Antigens; D007518:Isoantibodies; D008934:Mitogens