Changes in the cellular membrane surface coat of lymphocytes and thymocytes after incubation in vitro with cystein as revealed with electronmicroscopy.
Changes in the cellular membrane surface coat of lymphocytes and thymocytes after incubation with cystein in vitro were revealed with electronmicroscope, while performing the reaction with Ruthenium Red and Concanavaline A. Lymphocytes and thymocytes not incubated with cystein to which reaction with Ruthenium red and Cocanavaline A was applied have shown a well developed and preserved surface coat of the cellular membrane. Contrary to this finding when lymphocytes and thymocytes were incubated with cystein and thereafter treated with Ruthenium Red and Concanavaline A no reaction product on the surface of the cellular membrane was observed. The experimental results could indicate on the influence of cystein on the glycoside bonds.
D000818:Animals; D002462:Cell Membrane / Q000187:drug effects; D003208:Concanavalin A; D003545:Cysteine / Q000494:pharmacology*; D005260:Female; D008214:Lymphocytes / Q000187:drug effects* / Q000648:ultrastructure; D051381:Rats; D012430:Ruthenium Red; D013601:T-Lymphocytes / Q000187:drug effects* / Q000648:ultrastructure
D003208:Concanavalin A; D012430:Ruthenium Red; D003545:Cysteine