Selective activation of human beta-but not gamma-globin gene in human fibroblast x mouse erythroleukaemia cell hybrids.
The human alpha- and beta-globin genes have been activated in MEL X human fibroblast cell hybrids. However, even though the human gamma- and beta-globin genes are closely linked and were shown in these hybrid clones to be present in approximately equal numbers, no human gamma-globin mRNA was produced. Thus, the human beta- and gamma-globin genes in these cells are differentially regulated apparently by a positive regulatory factor(s) specific for individual globin genes.
D000818:Animals; D002460:Cell Line; D005796:Genes; D008040:Genetic Linkage; D005914:Globins / Q000235:genetics*; D006822:Hybrid Cells / Q000378:metabolism*; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000235:genetics; D004915:Leukemia, Erythroblastic, Acute / Q000235:genetics*; D007942:Leukemia, Experimental / Q000235:genetics; D046911:Macromolecular Substances; D012333:RNA, Messenger / Q000235:genetics; D014158:Transcription, Genetic
D007527:Isoenzymes; D046911:Macromolecular Substances; D012333:RNA, Messenger; D005914:Globins; D007770:L-Lactate Dehydrogenase