title: Reaginic antibody production to Ascaris suum allergen, ASC-1. I. The function of glutaraldehyde-polymerized antigen in the induction of reaginic (IgE) antibodies in the rat.
abstract: Rats immunized with a purified Ascaris suum allergen (Asc-1) or with its dinitrophenylated derivate (DNP-Asc-1) produced high levels of reaginic (IgE) antibodies. A second injection of antigen given 30 days later did not result in an anamnestic IgE antibody response. Immunization of adult-thymectomized, lethally-irradiated and bone-marrow reconstituted (ATxB) rats with soluble Asc-1 or DNP-Asc-1 failed to stimulate reaginic antibody production. The administration of glutaraldehyde-polymerized antigen induced in some but not all ATxB rats, low but detectable levels of IgE antibodies. These levels increased following a second injection of nonpolymerized antigen in A1 (OH)3 gels. Priming of animals with polymerized carrier and Bordetella pertussis did not stimulate a primary anticarrier IgE response but led to an enhanced antihapten IgE response following the administration of soluble DNP-Asc-1 in A1(OH)3. The results are consistent with the notion that a sharply reduced but clearly functional T-derived helper cell population could be triggered by the polymerized but not by the soluble form of the immunogen.
mesh_terms: D000485:Allergens; D000818:Animals; D000906:Antibodies; D000917:Antibody Formation; D001200:Ascaris / Q000276:immunology*; D003601:Cytotoxicity Tests, Immunologic; D004136:Dinitrobenzenes / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D007073:Immunoglobulin E / Q000096:biosynthesis*; D009578:Nitrobenzenes / Q000276:immunology*; D011108:Polymers; D011828:Radiation Chimera; D051381:Rats; D011935:Reagins / Q000096:biosynthesis* / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D013934:Thymectomy
chemical_list: D000485:Allergens; D000906:Antibodies; D004136:Dinitrobenzenes; D009578:Nitrobenzenes; D011108:Polymers; D011935:Reagins; D007073:Immunoglobulin E