Human T gamma globin chain is a variant of A gamma chain (A gamma Sardinia).
Isoelectric focusing, cellulose acetate electrophoresis, and carboxymethylcellulose chromatography in the presence of Nonidet P-40 allow the separation of pure gamma chains into two fractions. Amino acid analysis of their cyanogen bromide fragment 3 (gamma CB3) identifies these fractions as the separated G gamma (Gly-136) and A gamma (Ala-136) globin chains. Fingerprint and amino acid analyses of the gamma Tp9 tryptic peptide from the purified A gamma and G gamma fractions from two different patients demonstrate that the commonly occurring gamma Sardinia variant (gamma 75 isoleucine leads to threonine), also known as T gamma chain, has alanine in position 136. From this analysis we suggest that the T gamma gene is an allele of the A gamma locus (A gamma Sardinia) rather than a third gamma locus.
D000483:Alleles; D000595:Amino Acid Sequence; D003902:Detergents; D004588:Electrophoresis, Cellulose Acetate; D005914:Globins / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007525:Isoelectric Focusing; D009154:Mutation; D013789:Thalassemia / Q000097:blood
D003902:Detergents; D005914:Globins