title: Quantitation of precipitating antibodies to certain soluble nuclear antigens in SLE.
abstract: Electrophoresis in polyacrylamide gels in the presence of sodium dodecyl sulfate was used to separate and quantitate the components of a washed immune precipitate. Serum was from patients with systemic lupus erythematosus known to have antibodies to soluble nuclear ribonucleoprotein (RNP) or to a soluble nuclear non-nucleic acid protein (Sm). Amounts of antibody that was predominantly IgG ranged from 0.2 to 8 mg/ml of patients' serum, and in some cases accounted for over 20% of the total serum IgG. Results demonstrate that some patients respond to the disease by producing large amounts of a specific antibody, and that these antibodies can contribute significantly to hypergammaglobulinemia.
mesh_terms: D000974:Antibodies, Antinuclear / Q000032:analysis*; D000917:Antibody Formation; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006801:Humans; D006942:Hypergammaglobulinemia / Q000276:immunology; D007074:Immunoglobulin G / Q000032:analysis; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D011234:Precipitins / Q000032:analysis*
chemical_list: D000974:Antibodies, Antinuclear; D007074:Immunoglobulin G; D011234:Precipitins