The effect of insulin and hydrocortisone on the isolated rat liver.
Isolated rat livers were perfused at 35 degrees C with bovine serum albumin (40 g/l) in Krebs Ringer bicarbonate buffer, or with the same solution containing insulin (0.22 x 10(-6) mol/l), hydrocortisone (0.068 x 10(-3) mol/l), or both hormones together. Observations on the synthesis of bile and on perfusate levels of potassium, aspartate aminotransferase, urea and glucose showed that the presence of insulin and/or hydrocortisone had no beneficial effect on the perfused rat liver in vitro. There is little justification of the isolated liver.
D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D001646:Bile / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006854:Hydrocortisone / Q000494:pharmacology*; D066298:In Vitro Techniques; D007328:Insulin / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D008297:Male; D010477:Perfusion; D011188:Potassium / Q000378:metabolism; D051381:Rats; D014508:Urea / Q000378:metabolism
D007328:Insulin; D014508:Urea; D001219:Aspartate Aminotransferases; D005947:Glucose; D011188:Potassium; D006854:Hydrocortisone