Alkaline phosphatase activity in a strain of Bacterionema matruchotii.
Protein from the soluble fraction of Bacterium matruchotii cells propagated in a medium containing no added inorganic phosphate was fractionated by gel permeation chromatography. The bulk of phosphatase activity assayed at pH 8.0 was found in fractions equivalent to a molecular weight of 6 x 10(5) daltons. Substrate saturation kinetics indicate at Km of 0.75 mM for p-nitrophenylphosphate. Activity was stimulated more than two-fold by Zn++ at 1 mM, but was significantly reduced by EDTA, Ca++ and inorganic phosphate. The enzyme(s) shows negligible activity at pH below 6.0 and has a narrow optimum between 7.5 and 8.5.
D000191:Actinomycetaceae / Q000201:enzymology*; D000469:Alkaline Phosphatase / Q000378:metabolism*; D003470:Culture Media; D010710:Phosphates / Q000378:metabolism
D003470:Culture Media; D010710:Phosphates; D000469:Alkaline Phosphatase