title: Structure-independent nucleotide sequence analysis.
abstract: Substitution of inosine for granosine in the nucleic acid fragments synthesized for the sequencing of RNA effectively prevents the formation of secondary structures during electrophoretic analysis. Consequently, the mobility of each fragment in the sequencing gel is a strict function of its molecular weight. Inosine substitution should markedly improve the resolution that can be obtained in the sequencing of DNA as well as RNA.
mesh_terms: D001483:Base Sequence; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D006150:Guanine Nucleotides / Q000032:analysis; D006860:Hydrogen Bonding; D007292:Inosine Nucleotides / Q000032:analysis; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D012367:RNA, Viral / Q000032:analysis*; D014779:Virus Replication
chemical_list: D006150:Guanine Nucleotides; D007292:Inosine Nucleotides; D012367:RNA, Viral