Muscarinic cholinergic receptors in murine lymphocytes: demonstration by direct binding.
Using [3H]quinuclidinyl benzilate as a specific cholinergic muscarinic ligand, it has been demonstrated that lymphocytes have muscarinic binding sites. There are approximately 200 sites per cell and the dissociation constant for quinuclidinyl benzilate is approximately 1 X 10(-9) M. Quinuclidinyl benzilate receptor binding is blocked by atropine and oxotremorine.
D000818:Animals; D001285:Atropine / Q000378:metabolism; D001667:Binding, Competitive; D008024:Ligands; D008214:Lymphocytes / Q000378:metabolism*; D008297:Male; D051379:Mice; D010095:Oxotremorine / Q000378:metabolism; D011812:Quinuclidines / Q000378:metabolism; D011950:Receptors, Cholinergic / Q000378:metabolism*; D011976:Receptors, Muscarinic / Q000378:metabolism*
D008024:Ligands; D011812:Quinuclidines; D011950:Receptors, Cholinergic; D011976:Receptors, Muscarinic; D010095:Oxotremorine; D001285:Atropine