Immune reactivity of RBL-3, a nonproducer leukemia induced by Rauscher murine leukemia virus in C57BL/6 mice.
A cultured line of RBL-3 leukemia, induced by Rauscher murine leukemia virus (R-MuLV) in C57BL/6 mice, was found to be a virus nonproducer. An NB tropic virus indistinguishable from R-MuLV, however, was recovered after treatment with 5-iodo-2'-deoxyuridine (optimal concentration, 200 microgram/ml). Dexamethasone treatment slightly boosted the production of the activated virus but did not by itself activate the virus. RBL-3 cells were found to have Fc receptors but no surface immunoglobulin or Thy 1.2 antigen. RBL-3 cells exhibited the Friend-Moloney-Rauscher virus-specific transplantation antigen as shown by its immunosensitivity and immunogenicity in tumor rejection experiments. Crude membrane and crude soluble antigen preparations obtained from RBL-3 cell membranes retained the capacity to induce tumor rejection.
D000818:Animals; D000956:Antigens, Viral / Q000008:administration & dosage; D002478:Cells, Cultured; D005260:Female; D006084:Graft Rejection; D006649:Histocompatibility Antigens; D007065:Idoxuridine / Q000494:pharmacology; D007942:Leukemia, Experimental / Q000209:etiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D011925:Rauscher Virus / Q000276:immunology*; D014185:Transplantation, Isogeneic; D014412:Tumor Virus Infections / Q000209:etiology; D014764:Viral Proteins / Q000276:immunology; D014779:Virus Replication / Q000187:drug effects
D000956:Antigens, Viral; D006649:Histocompatibility Antigens; D014764:Viral Proteins; D007065:Idoxuridine