Cycloheximide-induced mitotic delay in Physarum polycephalum.
Cycloheximide pulses applied to Physarum polycephalum surface plasmodia delay mitosis. Pulses applied in G2 cause a delay of mitosis which is linearly dependent on the phase in the cell cycle at which the pulse is applied. A 30 min pulse of 10 micrograms/ml cycloheximide starting in G2 at time t after mitosis induces an excess delay (delay in excess of pulse duration) of the next mitosis of (0.55) t-1.3 h. The excess delays induced by 7 h pulses during G2 are at most 1 h larger. Pulses applied less than 30 min before mitosis induce only small delays.
D003513:Cycloheximide / Q000494:pharmacology*; D007399:Interphase / Q000187:drug effects; D008938:Mitosis / Q000187:drug effects*; D010804:Physarum / Q000187:drug effects* / Q000254:growth & development; D013997:Time Factors
D003513:Cycloheximide