The use of rhodamine-latex particles in B-cell determinations.
A simple, inexpensive, and easily visible procedure for labeling phagocytic cells in B-cell preparations is discussed. The procedure involves adding rhodamine-latex particles to lymphocyte preparations, then simultaneously looking for the presence of ingested latex particles in contaminating phagocytic cells and for fluorescein labeled surface immunoglobulins. This differentiation of cells allows one to correct for the true percentage of B cells.
D000293:Adolescent; D000328:Adult; D001402:B-Lymphocytes; D002462:Cell Membrane / Q000276:immunology; D005260:Female; D006801:Humans; D007136:Immunoglobulins; D007840:Latex; D007958:Leukocyte Count / Q000379:methods; D008297:Male; D008863:Microspheres; D010586:Phagocytes; D012235:Rhodamines; D014966:Xanthenes
D007136:Immunoglobulins; D007840:Latex; D012235:Rhodamines; D014966:Xanthenes