Matrix formation in craniofacial cartilages of the rat. [35S]-sulfate incorporation studies.
Various cartilaginous zones in the craniofacial region of rats were studied using radioactive sulfate incorporation. Chondroitinsulfate represents a major part of the cartilaginous matrix. By measuring the cpm/DNA ratio in the tissue samples, one will get an expression of the matrix formation activity of the zones investigated. An increase in the cpm/DNA ratio was found in all zones between the 14th and the 17th day. This increase corresponds with a period of great proliferative activity. Autoradiography of the mandibular condyle revealed no sulfate labelling centrally indicating that this structure grows purely by surface apposition.
D000818:Animals; D001345:Autoradiography; D001857:Bone Matrix / Q000000981:diagnostic imaging / Q000502:physiology*; D002356:Cartilage / Q000000981:diagnostic imaging / Q000254:growth & development*; D002455:Cell Division; D004247:DNA / Q000378:metabolism; D005260:Female; D008297:Male; D008445:Maxillofacial Development; D011859:Radiography; D051381:Rats; D013431:Sulfates / Q000378:metabolism; D013462:Sulfur Radioisotopes
D013431:Sulfates; D013462:Sulfur Radioisotopes; D004247:DNA