Effect of iodoacetate on T lymphocytes of young AKR mice.
The influence of sulphydryl inhibitor iodoacetate on properties characteristically associated with T-lymphocytes was examined in young AKR mice. Thymocytes from mice receiving 100 microgram of iodoacetate showed a decrease in cortisone sensitivity, and responded more vigorously to phytohemagglutinin (PHA). Spleen cells from treated mice also exhibited greater reactivity to both PHA and concanavalin A (Con A). The uptake of 14C-iodoacetate by the thymus, spleen, lymph nodes, bone marrow, liver and kidneys also was investigated. One hour, 2 h and 18 h after the intraperiotoneal injection of 10-3 M iodoacetate, the thymus demonstrated a higher incorporation of the labelled compound than did the spleen, lymph nodes or other organs examined. These findings provide further evidence to indicate that the immuno-enhancement observed following iodoacetate treatment is related to its effect on T lymphocytes.
D000818:Animals; D003348:Cortisone / Q000494:pharmacology; D005260:Female; D066298:In Vitro Techniques; D007461:Iodoacetates / Q000378:metabolism / Q000494:pharmacology*; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008806:Mice, Inbred AKR; D013601:T-Lymphocytes / Q000187:drug effects*; D014018:Tissue Distribution
D007461:Iodoacetates; D003348:Cortisone