title: Existence of only a single functional pool of adenosine triphosphate in human erythrocytes.
abstract: The question of whether separate "membrane" and "soluble" pools of ATP exist in erythrocytes has been examined. Phosphoglycerate kinase (EC 2.7.2.3)-derived ("membrane") ATP was labeled by short-term incubation with inorganic [32P]phosphate. Pyruvate kinase (EC 2.7.1.40)-derived ("soluble")ATP is not labeled under these circumstances. The specific activity of the gamma-phosphate of "soluble" ATP was then evaluated by the addition of 2-deoxyglucose and measurement of the specific activity of 2-deoxyglucose-6-[32P]phosphate formed. This specific activity was essentially the same as the overall specific activity of erythrocyte ATP gama-phosphate, indicating that no functional pools of phosphoglycerate kinase-derived and pyruvate kinase-derived ATP exist in erythrocytes.
mesh_terms: D000255:Adenosine Triphosphate / Q000097:blood*; D003847:Deoxyglucose / Q000097:blood; D004163:Diphosphoglyceric Acids / Q000097:blood; D004912:Erythrocytes / Q000378:metabolism*; D006593:Hexokinase / Q000097:blood; D006801:Humans; D010735:Phosphoglycerate Kinase / Q000097:blood; D011770:Pyruvate Kinase / Q000097:blood; D012995:Solubility
chemical_list: D004163:Diphosphoglyceric Acids; D000255:Adenosine Triphosphate; D003847:Deoxyglucose; D006593:Hexokinase; D011770:Pyruvate Kinase; D010735:Phosphoglycerate Kinase