Epidermal growth factor and the multiplication of cultured human epidermal keratinocytes.
The culture lifetime of epidermal cells of newborn humans is increased from 50 to 150 generations by adding to the medium epidermal growth factor, a polypeptide mitogen. EGF seems to delay senescence of the cells by maintaining them in a state further removed from terminal differentiation. This effect is revealed by a greater ability of the cells to survive subculture and initiate new colonies, but not necessarily by an increased growth rate.
D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured / Q000187:drug effects*; D002999:Clone Cells / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D010455:Peptides / Q000494:pharmacology*; D012867:Skin / Q000166:cytology
D010455:Peptides; D004815:Epidermal Growth Factor