Production and characterization of a monoclonal antibody to chicken type I collagen.
We have shown that lymphocyte-myeloma cell hybridization can be used to produce large amounts of extremely high-titer specific antibodies against type I collagen, a macromolecule normally of low immunogenicity. In a passive hemagglutination assay the antibody had a high titer against chicken type I collagen but showed no activity against chicken type II or rat type I collagen. By using a two-step fluorescence histochemical procedure on sections of embryonic chicken tibia, strong fluorescence was observed in the perichondrium and surrounding connective tissue (known to contain type I collagen) but not over the cartilage (characterized by type II collagen). When used in conjunction with Staphylococcus aureus as a solid phase immunoadsorbant, the antibody was shown to bind to labeled collagen synthesized in vitro by embryonic chicken calvaria.
D000818:Animals; D000917:Antibody Formation; D000918:Antibody Specificity; D001842:Bone and Bones / Q000378:metabolism; D002645:Chickens; D002999:Clone Cells / Q000276:immunology; D003094:Collagen / Q000096:biosynthesis / Q000276:immunology*; D006822:Hybrid Cells / Q000276:immunology; D051379:Mice; D008819:Mice, Nude / Q000276:immunology
D003094:Collagen