Enamel proteins: biosynthesis and chemistry.
Bovine ameloblast cell layer was incubated in 3H-proline containing medium and the incorporation of radioactivity into protein fractions of the cell and the medium was investigated by SDS- polyacrylamide gel electrophoresis. The results suggest that the ameloblasts synthesize and secrete a protein having a molecular weight of 25,000 daltons which is supposed to be the prototype of enamel proteins. This protein was degraded into dialyzable sizes by the cell extract. After a longer period of incubation another low molecular weight protein fraction appeared in the medium. Purification procedure with SDS gel filtration of the enamel protein is reported.
D000565:Ameloblasts / Q000201:enzymology / Q000378:metabolism; D000818:Animals; D002417:Cattle; D055598:Chemical Phenomena; D002621:Chemistry; D003746:Dental Enamel Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D010447:Peptide Hydrolases / Q000032:analysis / Q000378:metabolism; D011392:Proline / Q000378:metabolism; D012967:Sodium Dodecyl Sulfate
D003746:Dental Enamel Proteins; D012967:Sodium Dodecyl Sulfate; D011392:Proline; D010447:Peptide Hydrolases