Two chromatin fractions with different metabolic properties of non-histone proteins and of newly synthesized RNA.
Digestion of chromatin with micrococcal nuclease under mild conditions results in the release of a minor chromatin fraction showing an increased RNA and non-histone protein content, a fast turnover of the non-histone proteins and the presence of rapidly labelled heterogeneous nuclear RNA (hnRNA) with half-life of about 20 min. Further digestion of the chromatin leads to the elimination of about 19% of the initial chromosomal DNA, thus leaving a second chromatin fraction relatively resistant to nuclease attack. This fraction has a low protein and RNA content and contains only metabolically stable non-histone proteins. No differences in the histone complement of the two fractions was found except for a 40% deficiency of H1 in the minor fraction.
D000818:Animals; D002460:Cell Line; D002843:Chromatin / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D006224:Cricetinae; D006207:Half-Life; D007700:Kinetics; D004915:Leukemia, Erythroblastic, Acute; D051379:Mice; D012332:RNA, Heterogeneous Nuclear / Q000096:biosynthesis / Q000378:metabolism*; D014529:Uridine / Q000378:metabolism
D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D012332:RNA, Heterogeneous Nuclear; D014529:Uridine