Prostaglandin A1 induces differentiation in Friend erythroleukemia cells.
The effect of different prostaglandins and prostaglandin-metabolites on the growth and differentiation of Friend erythroleukemia cells (FLC) was evaluated. The prostaglandin-metabolites, thromboxane B2 and 6-keto PGF1 alpha, were completely inactive, while PGE1 inhibited slightly and PGF2 alpha stimulated the replication of FLC. PGA1 was found to be the most active compound. It profoundly inhibited the replication of both DMSO-treated and undifferentiated FLC. Most importantly, PGA1 alone induced differentiation in FLC, stimulating hemoglobin production over a five-day period. PGA1-stimulated differentiation was completely suppressed by the addition of 10(-6)M hydrocortisone. Finally, treatment of DMSO-differentiated cells with PGA1 (but no DMSO) prevented the return to the undifferentiated state.
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D004900:Erythroblasts / Q000187:drug effects* / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects*; D005622:Friend murine leukemia virus; D006454:Hemoglobins / Q000096:biosynthesis; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism; D007942:Leukemia, Experimental / Q000378:metabolism; D011448:Prostaglandin Antagonists / Q000494:pharmacology; D011454:Prostaglandins A / Q000008:administration & dosage / Q000494:pharmacology*; D011458:Prostaglandins E / Q000494:pharmacology; D011461:Prostaglandins F, Synthetic / Q000494:pharmacology
D006454:Hemoglobins; D011448:Prostaglandin Antagonists; D011454:Prostaglandins A; D011458:Prostaglandins E; D011461:Prostaglandins F, Synthetic; D006854:Hydrocortisone; D004121:Dimethyl Sulfoxide