A simple method for the cryopreservation of human lymphocytes at -80C.
A simple and inexpensive method for the freezing of human blood lymphocytes at -80 C is described. Lymphocytes were frozen in either polystyrene tubes or polyolefin bags. Optimal recovery of cells was obtained when 5% DMSO and 62% plasma were used as cryoprotectants. Viable cell recovery, as assessed by response to phyto hemagglutinin (PHA) and pokeweed mitogen (PWM), was greater than 80% after 60 days of storage. In addition, percentages of T and B lymphocytes were essentially unchanged. The excellent viable cell recovery in polyolefin bags suggests that it is possible to cryopreserve large numbers of lymphocytes in a single container.
D000475:Alkenes; D001402:B-Lymphocytes; D001793:Blood Preservation / Q000379:methods*; D002470:Cell Survival; D004121:Dimethyl Sulfoxide; D005615:Freezing; D006801:Humans; D007958:Leukocyte Count; D008213:Lymphocyte Activation; D008214:Lymphocytes; D010949:Plasma; D011137:Polystyrenes; D013601:T-Lymphocytes
D000475:Alkenes; D011137:Polystyrenes; D004121:Dimethyl Sulfoxide