Normal level of unscheduled DNA synthesis in Werner's syndrome fibroblasts in culture.
We investigated UV-induced unscheduled DNA synthesis (UDS) in skin fibroblasts from seven unrelated patients with clinically apparent Werner's syndrome (WS). WS cells exhibited greatly abbreviated in vitro lifespans, the extents of which ranged from about 20 to 50% of the normal. However, WS cells in early and senescent phases of growth showed the same quantity of DNA repair following UV exposure as did normal fibroblasts.
D000328:Adult; D002470:Cell Survival; D002675:Child, Preschool; D004260:DNA Repair; D004307:Dose-Response Relationship, Radiation; D005260:Female; D005347:Fibroblasts / Q000378:metabolism / Q000528:radiation effects; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D008875:Middle Aged; D014466:Ultraviolet Rays; D014898:Werner Syndrome / Q000235:genetics*