Junctional modulation in mouse embryonal carcinoma cells by Fab fragments of rabbit anti-embryonal carcinoma cell serum.
Mouse embryonal carcinoma PCC4 cells are connected by extensive gap and tight junctions. When the cells are incubated in a medium containing Fab fragments against embryonal carcinoma F9 cells, they round up and a process of junctional removal is initiated. In particular, gap junctions are internalized and after 30 hr of incubation with the anti-F9 Fab fragments both tight and gap junctions are no longer present at the cell surface; however, the cells are still in contact by means of small attachment sites.
D000818:Animals; D000912:Antibodies, Neoplasm / Q000008:administration & dosage*; D002460:Cell Line; D007140:Immunoglobulin Fab Fragments; D007365:Intercellular Junctions / Q000276:immunology* / Q000648:ultrastructure; D051379:Mice; D008854:Microscopy, Electron; D009374:Neoplasms, Experimental / Q000276:immunology; D011971:Receptors, Immunologic; D013724:Teratoma / Q000276:immunology* / Q000648:ultrastructure; D013997:Time Factors
D000912:Antibodies, Neoplasm; D007140:Immunoglobulin Fab Fragments; D011971:Receptors, Immunologic