Transcription of spacer sequences in genes coding for ribosomal RNA in Xenopus cells.
Untreated Xenopus cells synthesize RNA is increased and some sequences complementary to those of the nontranscribed spacer are found in the heavy shoulder of the 40S precursor rRNA peak. In such events transcription initiation seems to take place within the spacer because its middle and right BamHI endonuclease fragments are preferentially transcribed, whereas only few RNA sequences complementary to the left spacer fragment are found. It is concluded that at least some spacer regions contain promoters for transcription and can be transcribed either into a special class of "spacer transcripts" or into molecules covalently linked to rRNA precursor.
D000818:Animals; D003080:Cold Temperature; D004274:DNA, Recombinant; D005796:Genes; D008040:Genetic Linkage; D008970:Molecular Weight; D009694:Nucleic Acid Precursors / Q000096:biosynthesis*; D012332:RNA, Heterogeneous Nuclear / Q000378:metabolism; D012335:RNA, Ribosomal / Q000096:biosynthesis* / Q000235:genetics; D014158:Transcription, Genetic; D014981:Xenopus
D004274:DNA, Recombinant; D009694:Nucleic Acid Precursors; D012332:RNA, Heterogeneous Nuclear; D012335:RNA, Ribosomal