Protease-induced constriction in rats' bronchi.
A method is described which allows quantitative measurements of bronchomotor function in vitro in all parts of the tracheobronchial tree. With this method it was observed that chymotrypsin, trypsin and ficin elicted contraction in the intrapulmonary airways of rats. The response could be abolished or diminished by phentolamin and atropine. Physostigmine did not potentiate the response. It is concluded that chymotrypsin operates mainly through alpha-adrenoreceptors, whereas trypsin and ficin operate through both a alpha-adrenoreceptors and cholinoceptive receptors.
D000403:Airway Resistance / Q000187:drug effects; D000818:Animals; D007611:Aprotinin / Q000494:pharmacology; D001285:Atropine / Q000494:pharmacology; D001980:Bronchi / Q000187:drug effects*; D001982:Bronchial Diseases / Q000209:etiology; D002918:Chymotrypsin / Q000494:pharmacology; D003251:Constriction, Pathologic; D005260:Female; D005361:Ficain / Q000494:pharmacology; D008168:Lung / Q000503:physiopathology; D010447:Peptide Hydrolases / Q000494:pharmacology*; D010646:Phentolamine / Q000494:pharmacology; D010830:Physostigmine / Q000494:pharmacology; D051381:Rats; D014357:Trypsin / Q000494:pharmacology
D001285:Atropine; D007611:Aprotinin; D010830:Physostigmine; D010447:Peptide Hydrolases; D002918:Chymotrypsin; D014357:Trypsin; D005361:Ficain; D010646:Phentolamine