title: Human milk stimulates DNA synthesis and cellular proliferation in cultured fibroblasts.
abstract: Human milk contains a mitogenic factor that stimulates DNA synthesis and cell division in mouse and human fibroblasts in vitro. Milk at a concentration of 1% (vol/vol) is as active in stimulating DNA synthesis as is 5% (vol/vol) human serum and 10% (vol/vol) calf serum. The mitogenic activity of human milk is destroyed by incubation with trypsin and chymotrypsin. However, neither urea, guanidine hydrochloride-dithiothreitol, nor exposure to pH 1 will inactivate the milk-derived growth factor. Gel filtration and isoelectric focusing indicate that the mitogenic activity of human milk has a molecular weight between 14,000 and 18,000 and an isoelectric point between 4.4 and 4.7.
mesh_terms: D002455:Cell Division; D002478:Cells, Cultured; D002918:Chymotrypsin / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis*; D005347:Fibroblasts; D006133:Growth Substances / Q000378:metabolism*; D007526:Isoelectric Point; D008895:Milk, Human / Q000378:metabolism*; D008970:Molecular Weight; D014357:Trypsin / Q000494:pharmacology
chemical_list: D006133:Growth Substances; D004247:DNA; D002918:Chymotrypsin; D014357:Trypsin