Induction of erythropoietic colonies in a human chronic myelogenous leukemia cell line.
The ability of cells derived from the K562 cell line to generate erythropoietic colonies was studied. The K562 cell line was derived from a patient with chronic myelogenous leukemia 8 yr ago by Lozzio and Lozzio. Rare benzidine-positive colonies formed when these cells were cloned in plasma clots (3 +/- 1/10(4) cells), and their number was not substantially increased by the addition of erythropoietin (9.5 +/- 1/10(4) cells). Sodium butyrate was capable of markedly enhancing the number of benzidine-positive colonies (19.5 +/- 1/10(4) cells) formed, while the combination of sodium butyrate plus erythropoietin exerted a synergistic effect on erythropoietic colony formation (57 +/- 4/10(4) cells). The K562 cell line is a long-term culture system that contains human erythropoietic stem cells. This cell line should be useful in future studies on the cellular and molecular events associated with human erythroid cell differentiation.
D001560:Benzidines; D002087:Butyrates / Q000494:pharmacology; D002460:Cell Line; D002478:Cells, Cultured; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D004920:Erythropoiesis; D004921:Erythropoietin / Q000494:pharmacology; D006801:Humans; D007951:Leukemia, Myeloid / Q000097:blood*; D013997:Time Factors
D001560:Benzidines; D002087:Butyrates; D004921:Erythropoietin; D004121:Dimethyl Sulfoxide