Action of 5-bromodeoxyuridine on tooth germs "in vitro". II. - Effects on collagen synthesis.
The first lower molar tooth germs removed from 16-day-old mouse embryos were cultured for 2 days on a standard medium and then for 24 hours on the same medium containing BrdU (treated tooth germs) or not (controls). We attempted to study the effects of 5-Bromodeoxyuridine on the type I and type III collagen synthesis 24 (stage 16 + 4) and 72 (stage 16 + 6) hours after the incorporation of this thymidine analogue. At stage 16 + 4, type I and type III collagen were synthesized both in control and BrdU-treated tooth germs. However BrdU induced quantitative modifications in the type I collagen synthesis which might be explained by modifications in the turnover of this type of collagen. At stage 16 + 6, the BrdU treatment resulted in the inhibition of the terminal differentiation of odontoblasts. Consequently, the normal amplification of collagen type I synthesis could not occur. However, both control and BrdU-treated tooth germs synthesized type I and type III collagen. Quantitatively, the synthesis of type III collagen was slightly affected by BrdU treatment.
D000818:Animals; D001973:Bromodeoxyuridine / Q000494:pharmacology*; D002845:Chromatography / Q000379:methods; D003094:Collagen / Q000032:analysis / Q000096:biosynthesis* / Q000145:classification / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005998:Glycine / Q000378:metabolism; D051379:Mice; D010455:Peptides / Q000032:analysis; D011392:Proline / Q000378:metabolism; D014083:Tooth Germ / Q000187:drug effects* / Q000378:metabolism
D010455:Peptides; D003094:Collagen; D011392:Proline; D001973:Bromodeoxyuridine; D005998:Glycine