[Genetic control of the H-2 region of the cell surface: cationic amino groups in the periphery of T lymphocytes].
Spleen lymphocytes and erythrocytes from congenic mice of diffrent haplotypes were characterized on a precise biophysical basis (the anodic electrophoretic mobility, EPM) to correlate any subtle differences in the cell surface topochemistry with the H-2 specificity. Spleen T lymphocytes from A.CA (H-2f) and A.SW (H-2s) mice exhibited high values EPM, which were significantly different. In contrast, significant differences in the EPM of B cells and erythrocytes were not observed. Cell electrophoresis of spleen T lymphocytes (without " contaminating " B cells), before and after the chemical modification of the cell surface by treatment with small/non-toxic concentrations of maleic anhydride, showed the number of lysine side chain amino-groups in the periphery of cells with H-2f specificity to be about twice those on cells with H-2s haplotype. Such a difference was observed both in the case of premature T lymphocytes and mature T lymphocytes. The difference in the number of cationogenic amino-groups in the cell periphery contributing a positive charge, would explain the oberved difference in the EPM of H-2f and H-2s spleen T lymphocytes, and suggest that the macromolecules coded by the H-2 genes or other genes under H-2 control lead to delicate differences in the chemical composition of the surface membranes of cells of the two H-2 haplotypes, expressed only on high EPM lymphocytes of spleen (T or T-like cells).
D000818:Animals; D002414:Cations, Monovalent; D002462:Cell Membrane / Q000276:immunology; D002465:Cell Movement; D005796:Genes; D006649:Histocompatibility Antigens / Q000032:analysis*; D008239:Lysine / Q000378:metabolism*; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology*
D002414:Cations, Monovalent; D006649:Histocompatibility Antigens; D008239:Lysine
Contrôle génétique de la région H2 sur la topographie chimique de la surface cellulaire: étude des groupements aminés cationiques à la périphérie des lymphocytes T