De novo cell-free synthesis of human interferon.
Biologically active human interferon was synthesized de novo in a cell-free mouse extract stimulated with messenger RNA from induced human fibroblasts. The identity of the antiviral activity as human interferon was demonstrated by its antigenic and species specificity. Some characteristics of the cell-free synthesis were described.
D000818:Animals; D002474:Cell-Free System; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D066298:In Vitro Techniques; D007372:Interferons / Q000096:biosynthesis* / Q000276:immunology; D051379:Mice; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D013997:Time Factors
D007106:Immune Sera; D012333:RNA, Messenger; D007372:Interferons