Interaction of colony stimulating factor (CSF) from different sources with sepharose-concanavalin A.
Preparations from various sources (human urines, mouse placenta and pregnant uterus extract, and fibroblast conditioned medium) were examined comparatively with affinity chromatography on Sepharose-Concanavalin A for the presence of Colony Stimulating Factor (CSF). The preparations from all sources constantly revealed two protein peaks: the proteins of the first peak, without Concanavalin A affinity, contained CSF in the material from pregnant uterus and fibroblast conditioned medium, while human urine preparation was devoid of CSF: the inhibitor of CFUc growth, detected and titrated in this peak, probably does not explain the lack of CFUc growth. The proteins from the second peak, specifically eluted with alpha-methyl D-glucopyranoside revealed CSF in all the examined preparations.
D000818:Animals; D002846:Chromatography, Affinity; D003115:Colony-Stimulating Factors / Q000378:metabolism* / Q000652:urine; D003208:Concanavalin A; D005260:Female; D005347:Fibroblasts / Q000378:metabolism*; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D051379:Mice; D010920:Placenta / Q000378:metabolism*; D011134:Polysaccharides; D011247:Pregnancy; D012685:Sepharose; D014599:Uterus / Q000378:metabolism*
D003115:Colony-Stimulating Factors; D006023:Glycoproteins; D011134:Polysaccharides; D003208:Concanavalin A; D012685:Sepharose