Anti-DNP antibody response after the topical application of DNFB in mice.
A single painting or daily paintings for 5 days with dinitrofluorobenzene (DNFB) on the abdominal skin of mice induced both contact sensitivity, detectable by ear swelling, and, hapten-reactive helper T cells, detectable by the augmented anti-bovine serum albumin (BSA) antibody response on challenge with dinitrophenyl-BSA. Contact sensitivity was induced within 7 days and helper activity within 14 days after the sensitization. Anti-hapten antibody response in the spleen or regional lymph nodes of such mice, however, was negligibly small during the 15 days after a single painting. Failure to respond with anti-hapten antibody production of mice given only a single painting was shown to be due to the shortage of B cells reactive to the hapten. Daily paintings for 5 days did not necessarily result in the augmented antibody response. By contrast, a strong anti-hapten antibody response was observed in mice receiving two paintings at an interval of 10 days. In these mice, hapten-specific B memory cells as well as hapten-reactive T cells were detected. Thus, the anti-hapten antibody response after the topical applciation of simple chemicals may depend upon the priming of B cells, and the response must be mediated by the cooperation of T and B cells both reactive to the antigen.
D000287:Administration, Topical; D000818:Animals; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology; D003877:Dermatitis, Contact / Q000276:immunology; D004139:Dinitrofluorobenzene / Q000008:administration & dosage / Q000276:immunology*; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D006241:Haptens; D006801:Humans; D008211:Lymphocyte Cooperation; D051379:Mice; D009578:Nitrobenzenes / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D013997:Time Factors
D006241:Haptens; D009578:Nitrobenzenes; D004139:Dinitrofluorobenzene