Semisynthesis of human somatotropin analogs.
Complementation of the natural NH2-terminal 134-amino acid fragment of the reduced, carbamoylmethylated human somatotropin with synthetic analogs of COOH-terminal fragments of 57, 42, or 38 amino acids of reduced, carbamoyl-methylated human somatotropin has been investigated. It was found that synthetic fragments of 57 and 42 amino acids gave recombinants with full growth-promoting activity, whereas attempts to obtain a recombinant with the synthetic 38-amino acid fragment were unsuccessful. The synthesis of two analogs of the COOH-terminal fragment of human somatotropin, human [N]e170, Ala165,182,189] somatotropin-(150-191) and human [Nle170, Ala165,182,189] somatotropin-(154-191), is herein described.
D000595:Amino Acid Sequence; D001681:Biological Assay; D013006:Growth Hormone / Q000096:biosynthesis*; D006801:Humans; D010446:Peptide Fragments; D013329:Structure-Activity Relationship
D010446:Peptide Fragments; D013006:Growth Hormone