Generation of T helper cells in vitro. III. Helper cell culture-derived factors are related to alloantigens coded for the I region of the H-2 major histocompatibility complex.
Thymocyte-macrophage cultures primed with carrier protein release an alloantigen-related factor that enhances the anti-hapten plaque-forming cell response of hapten-primed spleen cells in vitro. Use of immunoadsorbant columns made with a variety of alloantisera indicates that the relevant antigens in this system are coded for the the H-2 major histocompatibility locus in mice. The data indicate that in H-2d and H-2k strains the important genetic regions are in the I region between the K region and the I-C subregion and suggest, based on current understanding of Ia specificities, that the I-A subregion codes for these antigens.
D000818:Animals; D002478:Cells, Cultured; D006649:Histocompatibility Antigens; D007106:Immune Sera / Q000494:pharmacology; D007164:Immunosorbents / Q000494:pharmacology; D007519:Isoantigens; D008211:Lymphocyte Cooperation; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D013601:T-Lymphocytes / Q000276:immunology*
D006649:Histocompatibility Antigens; D007106:Immune Sera; D007164:Immunosorbents; D007519:Isoantigens