Characterization of cell populations used for studies of antibodies to I-region products.
Mononuclear cells from peripheral blood contained at least four different cell types: monocytes, T lymphocytes, B lymphocytes, and lymphocytes with Fc receptors. Monocytes represented 41% of B-cell-enriched preparations and gave rise to extra reactions due to monocyte antibodies in allo immune sera. Polyvalent anti-Ig stained 88% of B-enriched-cells. However, 58% of these cells had IgG bound to Fc receptors, and only 42% had surface Ig stable after brief incubation at 37 degrees C. Further studies are required to determine to what extent the antibodies reacting with such B-cell preparations are also heterogeneous.
D000906:Antibodies; D000941:Antigens / Q000302:isolation & purification; D001402:B-Lymphocytes / Q000276:immunology*; D001666:Binding Sites, Antibody; D002469:Cell Separation; D006801:Humans; D007141:Immunoglobulin Fc Fragments; D009000:Monocytes / Q000276:immunology; D013601:T-Lymphocytes
D000906:Antibodies; D000941:Antigens; D007141:Immunoglobulin Fc Fragments