[Cellular interaction in the pokeweed-mitogen induced terminal differentiation to polyclonal immunoglobulin production in human B lymphocytes in vitro].
The differentiation of human B lymphocytes in vitro into Ig secreting cells, as measured by a radioimmunoassay rendered specific to IgG and IgM, in response to a polyclonal stimulant, requires the participation of several cellular types. Upon poleweed-mitogen stimulation, B-cell enriched and monocyte containing cell suspensions prepared by elution of nylon wool adherent cells, when mixed with various concentrations of T cells purified by filtration through nylon wool column and depletion of EAC-rosette forming cells, synthesize 5 to 20 times more Ig than non separated lymphocytes. These experiments which demonstrate the cooperation of certain T cells also suggest that other cells eliminated by nylon wool adherence act as suppressor cells. The cooperation of these T cells is independent of their proliferation and can be obtained with allogeneic T cell.
D001402:B-Lymphocytes / Q000276:immunology*; D006801:Humans; D007111:Immunity, Cellular; D007070:Immunoglobulin A / Q000096:biosynthesis*; D007074:Immunoglobulin G / Q000096:biosynthesis*; D007075:Immunoglobulin M / Q000096:biosynthesis*; D037102:Lectins / Q000494:pharmacology*; D008213:Lymphocyte Activation
D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D037102:Lectins
Interactions cellulaires lors de la synthése d'immunoglobulines par les lymphocytes B humains aprés stimulation par le PWM in vitro