title: Analysis of malignancy in human cells: malignant and transformed phenotypes are under separate genetic control.
abstract: Human cell hybrids derived from malignant HeLa and normal fibroblast parental cells expressed many of the transformed properties of the HeLa parent but their tumor-producing capability was suppressed. Hybrids derived from HeLa/HeLa fusions retained both their transformed and malignant phenotypes. Thus, an apparent separation of the control of the transformed versus malignant phenotype is indicated. Furthermore, several transformed properties--including lack of density-dependent inhibition of growth, lectin agglutination, lowered requirement for serum growth factors, and anchorage independence--are expressed coordinately in the nontumorigenic hybrids. This finding suggests that none of these properties by themselves, or in concert, endows a cell with tumorigenic potential.
mesh_terms: D000818:Animals; D001769:Blood; D002449:Cell Aggregation; D002455:Cell Division; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000473:pathology*; D003208:Concanavalin A / Q000494:pharmacology; D003260:Contact Inhibition; D003470:Culture Media; D006133:Growth Substances; D006801:Humans; D006822:Hybrid Cells / Q000473:pathology; D051379:Mice; D009374:Neoplasms, Experimental / Q000235:genetics*
chemical_list: D003470:Culture Media; D006133:Growth Substances; D003208:Concanavalin A