title: Compartmentation of redox metabolites in the anterior eye segment?
abstract: In bovine corneal epithelium, stroma, and aqueous humor the levels of ascorbic acid (ASC) and dehydroascorbic acid (DHA) were investigated. Two methods were used, the photometric assay with 2,6-dichlorophenolindophenol and the formation of osazone by 2,4-dinitrophenylhydrazine. The ASC levels in the corneal epithelium and aqueous humor were found to be in the millimolar range, the ASC/DHA ratio being about 10. The stromal ASC and DHA levels were much lower, with a ratio of 0.7. ASC and DHA had similar levels and ratios to those of reduced and oxidized glutathione (GSH/GSSG) reported in the literature. In the corneal epithelium the redox ratio of glutathione was higher than that of ascorbic acid. Therefore, glutathione was supposed to reduce dehydroascorbic acid.
mesh_terms: D000818:Animals; D000867:Anterior Chamber / Q000378:metabolism*; D001082:Aqueous Humor / Q000378:metabolism; D001205:Ascorbic Acid / Q000378:metabolism; D002417:Cattle; D003315:Cornea / Q000378:metabolism; D003683:Dehydroascorbic Acid / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism
chemical_list: D005978:Glutathione; D001205:Ascorbic Acid; D003683:Dehydroascorbic Acid