title: Polyamine-pyridoxal Schiff bases in urine.
abstract: Schiff bases of the diamines 1,3-diaminopropane, putrescine, and cadaverine and the polyamines spermidine and spermine with pyridoxal or pyridoxal phosphate occur in human urine, as shown by gas chromatographic/mass spectrometric selected ion-monitoring techniques. By use of synthetic standards, procedures were devised for conversion of the Schiff bases to stable derivatives amenable to gas chromatographic/mass spectrometric analysis. These procedures involve borohydride reduction of the C = N double bond, hydrolytic removal of the phosphate group, chromatographic separation from the bulk of urinary constituents, and trifluoroacetylation of polar functional groups. The levels of the polyamine-pyridoxal Schiff bases were estimated to be in the range of pmol/ml or urine.
mesh_terms: D000328:Adult; D002103:Cadaverine / Q000652:urine; D055598:Chemical Phenomena; D002621:Chemistry; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007536:Isomerism; D011073:Polyamines / Q000652:urine*; D011700:Putrescine / Q000652:urine; D011730:Pyridoxal / Q000652:urine*; D011732:Pyridoxal Phosphate / Q000652:urine*; D012545:Schiff Bases / Q000652:urine*; D013095:Spermidine / Q000652:urine; D013096:Spermine / Q000652:urine
chemical_list: D011073:Polyamines; D012545:Schiff Bases; D013096:Spermine; D011730:Pyridoxal; D011732:Pyridoxal Phosphate; D002103:Cadaverine; D013095:Spermidine; D011700:Putrescine