Growth of Madin-Darby canine kidney epithelial cell (MDCK) line in hormone-supplemented, serum-free medium.
Madin-Darby canine kidney (MDCK) cells can grow in synthetic medium supplemented with five factors--insulin, transferrin, prostaglandin E1, hydrocortisone, and triiodothyronine--as a serum substitute. These five factors permit growth for 1 month in the absence of serum and a growth rate equivalent to that observed in serum-supplemented medium. Dibutyryl adenosine 3',5'-cyclic monophosphate substitutes for prostaglandin E1 in the medium. Potential applications of the serum-free medium are discussed. The medium permits a defined analysis of the mechanisms regulating hemicyst formation by hormones and permits the growth of primary kidney epithelial cell cultures in the absence of fibroblast overgrowth.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002460:Cell Line; D003470:Culture Media; D004285:Dogs; D004848:Epithelium; D006728:Hormones / Q000008:administration & dosage; D007668:Kidney / Q000254:growth & development; D051379:Mice; D011459:Prostaglandins E, Synthetic / Q000494:pharmacology
D003470:Culture Media; D006728:Hormones; D011459:Prostaglandins E, Synthetic