Fractionation of human lymphocytes using rosette formation with papain-treated mouse erythrocytes.
Human B lymphocytes from rosettes with mouse erythrocytes. If the erythrocytes are pretreated with papain the rosettes are sufficiently stable to allow separation of T and B lymphocytes. The technique is complementary to the sheep erythrocyte rosette method, and gives cell populations with a similar degree of purity.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D002469:Cell Separation; D004912:Erythrocytes / Q000276:immunology*; D006801:Humans; D051379:Mice; D010206:Papain / Q000494:pharmacology*; D011947:Receptors, Antigen, B-Cell / Q000378:metabolism; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000276:immunology*
D011947:Receptors, Antigen, B-Cell; D010206:Papain