Cellular regulation of the metabolism of androgens in rat oral mucosa. III. Activation of delta 4-3-ketosteroid-5alpha-A ring reductase enzyme system by 5,5-diphenylhydantoin.
Systemic pretreatment of rats with diphenylhydantoin (DPH) or its addition into an in vitro assay increases 5alpha-reduction of testosterone by the oral mucosa. Enzyme kinetic studies showed that DPH binds to the enzyme and probably activates it by an allosteric mechanism.
D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase / Q000378:metabolism*; D000818:Animals; D003600:Cytosol / Q000201:enzymology; D004789:Enzyme Activation / Q000187:drug effects; D007700:Kinetics; D008297:Male; D009061:Mouth Mucosa / Q000166:cytology / Q000201:enzymology*; D010088:Oxidoreductases / Q000378:metabolism*; D010672:Phenytoin / Q000494:pharmacology*; D051381:Rats; D013739:Testosterone / Q000378:metabolism*
D013739:Testosterone; D010672:Phenytoin; D010088:Oxidoreductases; D013741:3-Oxo-5-alpha-Steroid 4-Dehydrogenase