Influence of freezing and storage methods on concentrations of brain DOPA and 5 HTP after pretreatment with a decarboxylase inhibitor.
The concentrations of DOPA and 5 hydroxytryptophan in rat brain were decreased by freezing on dry ice rather than in liquid nitrogen, and by a 5 minute delay in removing and freezing the brain by either method. The effect of post-mortem delay in freezing was greater for 5 HTP than for DOPA.
D006916:5-Hydroxytryptophan / Q000032:analysis*; D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects; D002262:Carboxy-Lyases / Q000037:antagonists & inhibitors*; D004295:Dihydroxyphenylalanine / Q000032:analysis*; D004367:Dry Ice; D005615:Freezing; D008297:Male; D009584:Nitrogen; D011180:Postmortem Changes; D051381:Rats; D013997:Time Factors
D004367:Dry Ice; D004295:Dihydroxyphenylalanine; D006916:5-Hydroxytryptophan; D002262:Carboxy-Lyases; D009584:Nitrogen