Cell survival in V79 multicell spheroids treated with dehydroascorbate, 5-thio-D-glucose and 2-deoxy-D-glucose.
We found that dehydroascorbic acid (DHA), 5-thio-D-glucose (5TG) and 2-deoxy-D-glucose (2DG) were toxic to monolayers of V79 cells under hypoxic conditions at 37 degree C but not at 0 degree C. Very little toxicity was observed at either temperature under aerobic conditions. When spheroids (600 micrometer diameter) were treated with 7.5 mM of these chemicals for periods up to 3 days, the number of viable cells per spheroid dropped to 70% for DHA, 50% for 5TG and 25% for 2DG relative to controls. These survivals were reduced to much lower values when the experiments were repeated in glucose-free medium.
D001205:Ascorbic Acid / Q000031:analogs & derivatives*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003683:Dehydroascorbic Acid / Q000494:pharmacology*; D003837:Deoxy Sugars / Q000494:pharmacology*; D003847:Deoxyglucose / Q000494:pharmacology*; D005947:Glucose / Q000031:analogs & derivatives* / Q000494:pharmacology; D010100:Oxygen
D003837:Deoxy Sugars; D003847:Deoxyglucose; D005947:Glucose; D001205:Ascorbic Acid; D010100:Oxygen; D003683:Dehydroascorbic Acid