Delimitation of a promoter for RNA polymerase III by means of a functional test.
A Xenopus laevis DNA segment containing the structural gene for tRNA1Met and 22 base pairs at the 5' side of the gene is active in tRNA production. The DNA segment was ligated to sea urchin histone DNA and was also inserted into plasmid pCR1. Both recombinant DNAs were shown to produce mature tRNA1Met at a high rate when injected into centrifuged Xenopus oocytes.
D000818:Animals; D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000096:biosynthesis*; D005260:Female; D008715:Methionine; D009865:Oocytes / Q000378:metabolism; D012320:RNA Polymerase III / Q000096:biosynthesis*; D012343:RNA, Transfer / Q000096:biosynthesis; D014158:Transcription, Genetic; D014981:Xenopus
D004274:DNA, Recombinant; D012343:RNA, Transfer; D008715:Methionine; D012321:DNA-Directed RNA Polymerases; D012320:RNA Polymerase III; D004262:DNA Restriction Enzymes