[The in vitro biological evaluation of dental cements using elution and binding by bovine serum albumin (author's transl)].
In order to evaluate in vitro biological properties of dental cements sixteen different cements including zinc oxide eugenol (ZOE), EBA, resin, polycarboxylate, glass ionomer cement and calcium hydroxide base material were tested regarding elution and binding by bovine serum albumin (BSA). The persistency of elution over long time after subsequent transfer to fresh water was calculated at 210 nm absorbance with ultra violet spectrometer. Also the eluates obtained were analyzed by high performance liquid chromatograpy. The continuous leaching of eugenol from ZOE cement was observed. Binding of eluates from cement to BSA was evaluated spectrophotometrically by utilizing the competition with 2-(4' hydroxyphenylazo) benzoic acid (HABA). EBA cement showed the highest binding ability by BSA because of the leaching o-ethoxybenzoic acid from this cement. The relevance of protein and pulp toxicity is discussed.
D000818:Animals; D002417:Cattle; D003738:Dental Cements / Q000032:analysis*; D011076:Polycarboxylate Cement; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D015035:Zinc Oxide-Eugenol Cement
D003738:Dental Cements; D011076:Polycarboxylate Cement; D015035:Zinc Oxide-Eugenol Cement; D012710:Serum Albumin, Bovine