title: Elastases from human and canine granulocytes, II. Interaction with protease inhibitors of animal, plant, and microbial origin.
abstract: Inhibitors of animal, plant, and microbial origin were tested against human and canine granulocytic elastases. The trypsin-chymotrypsin inhibitors from dog submandibular glands, from soybeans (Bowman-Birk) and from chickpeas show strong interaction with these proteases (Ki = 10(-8) - 10(-9)M). The trypsin-kallikrein inactivator of bovine organs (Trasylol) is not active against granulocytic elastases or against human granulocytic cathepsin G. Elastatinal, a specific inhibitor of elastases, isolated from actinomycetes (Streptomyces griseoruber), forms stable complexes with elastase from human (Ki = 6.2 X 10(-6)M) and canine granulocytes (Ki = 1.1 X 10(-6)M). A possible therapeutic application of these inhibitors for the inactivation of granulocytic proteases, which are able to degrade connective tissue in different pathological states, is discussed.
mesh_terms: D000818:Animals; D007611:Aprotinin / Q000494:pharmacology; D004285:Dogs; D006098:Granulocytes / Q000201:enzymology*; D006801:Humans; D007962:Leukocytes / Q000201:enzymology*; D010196:Pancreatic Elastase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D010944:Plants; D013302:Streptomyces; D014361:Trypsin Inhibitors / Q000494:pharmacology
chemical_list: D014361:Trypsin Inhibitors; D007611:Aprotinin; D010196:Pancreatic Elastase