[Effects of capsules of Staphylococcus aureus on the rosette formation with B-lymphocytes and macrophages (author's transl)].
After extraction with guanidium chloride nonencapsulated Staphylococcus aureus (S-Gu) and yeast cells (Hefe-Gu) reduced the hemolytic complement activity of normal human and guinea pig sera much more (fig 1) than encapsulated S. aureus (KS-Gu). Following incubation in the fresh sera S-Gu, Hefe-Gu and guanidinium chloride-extracted S. epidermidis, contrary to KS-Gu, had activated C3 on their surface. Therefore, they formed rosettes with human B-lymphocytes (fig. 2,3,4) and peritoneal macrophages from guinea pigs. The adherence-reactions were less frequent. if the pre-incubation was conducted in the heat-inactivated (30 min, 56 degrees C) sera (table 1,2). After pre-incubation in the absence of serum only the protein A-positive S-Gu formed rosettes with B-lymphocytes.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D003176:Complement C3 / Q000378:metabolism; D004789:Enzyme Activation; D006146:Guanidines; D006168:Guinea Pigs; D006801:Humans; D007104:Immune Adherence Reaction; D008264:Macrophages / Q000276:immunology*; D013211:Staphylococcus aureus / Q000276:immunology* / Q000472:pathogenicity
D003176:Complement C3; D006146:Guanidines
Einfluss der Kapseln auf die Rosettenbildung von Staphylococcus aureus bei B-Lymphozyten und Makrophagen