Mechanism of phage Mu-1 integration: nalidixic acid treatment causes clustering of Mu-1-induced mutations near replication origin.
Frequencies of Mu-1-induced mutants of Escherichia coli have been compared under two different experimental conditions: cells in exponential growth and the same cells treated with nalidixic acid. The average of values obtained from the nalidixic acid-treated culture is 3 times higher than that obtained from the control. Individual ratios of the frequency of mutants in the two cultures yield decreasing values from 6 to 1, starting from the point of origin of DNA replication to the termini of DNA replication. These results are compatible with the idea that Mu-1 integrates at the replication fork.
D003090:Coliphages / Q000235:genetics*; D004261:DNA Replication; D004269:DNA, Bacterial / Q000378:metabolism*; D008242:Lysogeny; D009268:Nalidixic Acid / Q000494:pharmacology
D004269:DNA, Bacterial; D009268:Nalidixic Acid