Membrane-bound redox proteins of the murine Friend virus-induced erythroleukemia cell.
We have obtained and studied a 105,000-g pellet from T-3-Cl-2 cells, a cloned line of Friend virus-induced erythroleukemia cells. By difference spectrophotometry, the pellet was shown to contain cytochrome b5 and cytochrome P-450, hemeproteins that have been shown to participate in electron-transport reactions of endoplasmic reticulum and other membranous fractions of various tissues. The pellet also possesses NADH-cytochrome c reductase activity which is inhibited by anti-cytochrome b5 gamma-globulin, indicating the presence of cytochrome b5 reductase. This is the first demonstration of membrane-bound forms of these redox proteins in erythroid cells. Dimethyl sulfoxide-treated T-3-Cl-2 cells were also shown to possess membrane-bound cytochrome b5 and NADH-cytochrome c reductase activity. We failed to detect soluble cytochrome b5 in the 105,000-g supernatant fraction from homogenates of untreated or dimethyl sulfoxide-treated T-3-Cl-2 cells. In contrast, erythrocytes obtained from mouse blood were shown to possess soluble cytochrome b5 but no membrane-bound form of this protein. These findings are supportive of our hypothesis that soluble cytochrome b5 of erythrocytes is derived from endoplasmic reticulum or some other membrane structure of immature erythroid cells during cell maturation.
D000818:Animals; D002462:Cell Membrane / Q000201:enzymology / Q000378:metabolism*; D002472:Cell Transformation, Viral; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D003579:Cytochrome Reductases / Q000378:metabolism*; D003580:Cytochromes / Q000378:metabolism*; D005622:Friend murine leukemia virus; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism*; D007942:Leukemia, Experimental / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D008854:Microscopy, Electron; D009245:NADH Dehydrogenase / Q000378:metabolism*; D010084:Oxidation-Reduction; D011487:Protein Conformation; D014461:Ultracentrifugation
D003580:Cytochromes; D008565:Membrane Proteins; D003577:Cytochrome P-450 Enzyme System; D003579:Cytochrome Reductases; D009245:NADH Dehydrogenase