Cell contact to metal surface.
In order to observe cell contact with various metals, the pulp cells of human origin were grown on vacuum evaporated metal film in such tissue culture mediums as Medium 199 supplemented with 10% calf serum. Medium 199 (serum free), Hanks' balanced salt solution (serum free) and calcium and magnesium ions free balanced salt solution (serum free). Secondary electron images of the cells were observed. The following findings were recorded. 1) Differences of metal did not give any discernible effects in cell morphology, except cytotoxic metals which gave severe deteriorating effects to the cells. 2) Lack of serum appeared to give unfavourable effects to cell growth, but no decisive, positive evidences for cell contact were obtained. 3) The effect of calcium and magnesium ions on cell contact was not substantiated. Importance of accumulation of informations on cell to material contact was stressed in order to develop various biomaterials compatible to the cells. Further approaches to understand the phenomena in the subject were discussed.
D002118:Calcium; D002448:Cell Adhesion; D002455:Cell Division; D002470:Cell Survival; D002478:Cells, Cultured; D003470:Culture Media; D006801:Humans; D008274:Magnesium; D008670:Metals; D008855:Microscopy, Electron, Scanning
D003470:Culture Media; D008670:Metals; D008274:Magnesium; D002118:Calcium