C3-reacted sepharose: a preparative method for separating T and B lymphocytes.
Lymphocytes bearing complement receptors were separated from lymphocyte suspensions using C3-reacted Sepharose columns as solid phase immunoabsorbents. The retained CR+ lymphocytes were recovered by elution of the column with IgG fraction purified from a rabbit antiserum to human C3. This paper describes the method, its specificity and the yields of cells obtained.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D002469:Cell Separation / Q000379:methods*; D003176:Complement C3; D007074:Immunoglobulin G; D011817:Rabbits; D012685:Sepharose; D013601:T-Lymphocytes / Q000276:immunology
D003176:Complement C3; D007074:Immunoglobulin G; D012685:Sepharose