Human cell lines that elaborate colon-stimulating activity for the marrow cells of man and other species.
We have established two human cell lines which elaborate colony-stimulating activity (CSA) for at least four species: man, mouse, rabbit, and dog. One, GCT, was isolated from a lung metastasis of a fibrous histiocytoma; the other, RC4, from a monocyte-enriched fraction of normal blood. Medium conditioned by either GCT or RC4 cells was more potent in stimulating human marrow growth in vitro than was monocyte-conditioned medium or human leukocyte feeder layers. Fractionation of cell-line-conditioned medium by Sephacryl S-200 chromatography indicated that the maximum activity of the CSA for human marrow cells is eluted within the range of 30,000-40,000 daltons. These cells lines provide a continuous source of large quantities of conditioned medium for purification of CSA. Moreover, the invariable growth-supporting activity for all species tested and the high potency of cell-line CSA facilitates studies of its elaboration and biologic effects.
D000328:Adult; D000818:Animals; D001853:Bone Marrow / Q000254:growth & development*; D002460:Cell Line; D003115:Colony-Stimulating Factors / Q000302:isolation & purification / Q000494:pharmacology*; D003470:Culture Media; D004285:Dogs; D005260:Female; D006023:Glycoproteins / Q000494:pharmacology*; D006801:Humans; D007962:Leukocytes; D008297:Male; D051379:Mice; D008970:Molecular Weight; D009000:Monocytes; D011817:Rabbits
D003115:Colony-Stimulating Factors; D003470:Culture Media; D006023:Glycoproteins