Sodium ascorbate potentiates the growth inhibitory effect of certain agents on neuroblastoma cells in culture.
Mouse neuroblastoma (NB) cells in culture were more sensitive to sodium L-ascorbate than were rat glioma cells by the criterion of growth inhibition (due to cell death and reduction in cell division). Sodium L-ascorbate at nonlethal concentrations potentiated the effect of 5-fluorouracil (FUra), x-irradiation, bleomycin, RO20-1724, prostaglandin E1, and sodium butyrate on NB cells but did not produce such an effect on glioma cells. Sodium L-ascorbate did not enhance the effect of vincristine, 6-thioguanine, or 1-(2-chloroethyl)-3-cyclohexyl-1-nitrosourea (CCNU) except at higher drug doses and it reduced the cytotoxic effect of methotrexate and 5-(3,3-dimethyl-1-triazeno)-imidazole-4-carboxamide (DTIC) on NB cells. Sodium D-ascorbate produced effects similar to those produced by sodium L-ascorbate on NB cells. L-Ascorbic acid-2-sulfate (barium salt) affected neither the growth rate nor the effect of 5-FUra on NB cells. Glutathione, a reducing agent, was more toxic to NB cells in comparison to D- OR L-ascorbate; however, at a similar concentration it failed to potentiate the effect of 5-FUra on NB cells.
D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D001205:Ascorbic Acid / Q000494:pharmacology*; D002374:Catalase / Q000494:pharmacology; D002455:Cell Division / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004357:Drug Synergism; D005472:Fluorouracil / Q000494:pharmacology; D005910:Glioma / Q000473:pathology; D005978:Glutathione / Q000494:pharmacology; D007739:L Cells / Q000187:drug effects; D051379:Mice; D009447:Neuroblastoma / Q000473:pathology*; D051381:Rats; D014965:X-Rays
D000903:Antibiotics, Antineoplastic; D002374:Catalase; D005978:Glutathione; D001205:Ascorbic Acid; D005472:Fluorouracil