Sensitive, rapid, and specific bioassay for the determination of antilipogenic compounds.
A sensitive and rapid bioassay for the determination of the antilipogenic compounds cerulenin and CM-55 is described. The bioassay is based on the inhibitory effect of cerulenin and CM-55 on the in vivo luminescence of an aldehyde-requiring mutant of the marine bacterium Beneckea harveyi. A total quantity as low as 0.1 mug of cerulenin can be determined within 15 min with an error of +/-2%. The bioassay, as presented, is specific for compounds that are known to inhibit fatty acid biosynthesis and, as such, it might be used as a general screening method for the detection of antilipogenic compounds.
D000935:Antifungal Agents / Q000032:analysis*; D001681:Biological Assay / Q000379:methods*; D002569:Cerulenin / Q000031:analogs & derivatives / Q000032:analysis* / Q000494:pharmacology; D008163:Luminescent Measurements; D014733:Vibrio / Q000187:drug effects
D000935:Antifungal Agents; D002569:Cerulenin