Heteroantisera prepared against B lymphocytes at different stages of differentiation. I. Preparation of sera and cytotoxicity to lymphoid cells from different organs.
A routine method is described whereby anti-mouse B-cell antisera can be raised in rabbits. As judged by trypan blue dye exclusion these sera have negligible cytotoxicity to murine T lymphocytes but seem to detect differences in non-T lymphocytes in different organs. By sedimentation analysis some of the sera may detect (in addition to mature B cells) earlier precursors of mature B lymphocytes in bone marrow and spleen.
D000818:Animals; D000961:Antilymphocyte Serum / Q000302:isolation & purification*; D001402:B-Lymphocytes / Q000276:immunology*; D001853:Bone Marrow / Q000276:immunology; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D003601:Cytotoxicity Tests, Immunologic; D005260:Female; D008198:Lymph Nodes / Q000276:immunology; D008297:Male; D008722:Methods; D051379:Mice; D011817:Rabbits; D013154:Spleen / Q000276:immunology
D000961:Antilymphocyte Serum