Genetic transformation of Pseudomonas phaseolicola by R-factor DNA.
The bacterium Pseudomonas phaseolicola was successfully transformed, for the first time, with R-factors RSF1010 and pBR322 DNA by a calcium-shock and heat-pulse technique. Frequency of transformation for RSF1010 ranged from 0.8 x 10(-7) to 3.1 x 10(-6) and was ca. 0.4 x 10(-8) for pBR322.
D004269:DNA, Bacterial / Q000235:genetics; D011549:Pseudomonas / Q000235:genetics*; D011815:R Factors; D013307:Streptomycin / Q000494:pharmacology; D013752:Tetracycline / Q000494:pharmacology; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; D013752:Tetracycline; D013307:Streptomycin