Synthesis and thermal melting behavior of oligomer-polymer complexes containing defined lengths of mismatched dA-dG and dG-dG nucleotides.
Model DNA polymers containing heteroduplex regions of defined sequence and size were synthesized using polynucleotide phosphorylase and calf thymus terminal transferase. Heteroduplexes were of the form (dG)n-d(C12AmC-x), where m - 1-6, and (dG)n-d(C10GmC-x), where m = 1 and 3-5. Thermal melting studies of the model DNAs indicated that the heteroduplex regions did not disrupt the cooperative interaction between the flanking regions of dG-dC base pairs. thus, it is possible that the heteroduplex nucleotides are accommodated in a stacked helical structure.
D004254:DNA Nucleotidyltransferases; D003839:Deoxyadenosines / Q000031:analogs & derivatives / Q000138:chemical synthesis; D006150:Guanine Nucleotides / Q000138:chemical synthesis; D006358:Hot Temperature; D009691:Nucleic Acid Denaturation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis*; D009841:Oligonucleotides / Q000138:chemical synthesis*; D011089:Polydeoxyribonucleotides / Q000138:chemical synthesis*; D011117:Polyribonucleotide Nucleotidyltransferase; D013816:Thermodynamics
D003839:Deoxyadenosines; D006150:Guanine Nucleotides; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D011089:Polydeoxyribonucleotides; D004254:DNA Nucleotidyltransferases; D011117:Polyribonucleotide Nucleotidyltransferase