title: Identification, localization, and role of fibronectin in cultured bovine endothelial cells.
abstract: We have examined bovine aortic endothelial cell cultures for the presence of fibronectin, a high molecular weight cell-surface glycoprotein. Sparse cultures contain fibronectin only on dorsal cell surfaces at regions of cell-cell contact, as detected by immunofluorescence. In contrast, when the endothelial cells reached confluence as a highly contact-inhibited monolayer, fibronectin was detected in an extracellular matrix underneath the cell monolayer but not on top of the monolayer. Sodium dodecyl sulfate/polyacrylamide gel electrophoresis of isolated extracellular matrix revealed that a predominant component of the matrix is a protein of approximately 2.3 X 10(5) molecular weight, which has been identified as fibronectin.
mesh_terms: D001485:Basement Membrane / Q000378:metabolism; D001808:Blood Vessels / Q000166:cytology / Q000378:metabolism*; D002448:Cell Adhesion; D002460:Cell Line; D004727:Endothelium / Q000378:metabolism; D005110:Extracellular Space / Q000378:metabolism*; D006023:Glycoproteins / Q000302:isolation & purification / Q000378:metabolism*; D008565:Membrane Proteins / Q000302:isolation & purification / Q000378:metabolism*; D008970:Molecular Weight
chemical_list: D006023:Glycoproteins; D008565:Membrane Proteins