Early kinetics of B cell memory and the influence of T cells on the expression in vitro.
To study the early kinetics of B cell memory to sheep erythrocytes mouse spleen cells were cultured after short priming times in vivo. The influence of T cells on the expression of memory in vitro was investigated by treatment with anti-theta serum. Within 48 hours of in vivo priming part of the B cell population can differentiate into cells capable of IgG production after secondary antigen contact in vitro without the help of T cells. The presence of antigen was required for the in vitro development.
D000818:Animals; D000917:Antibody Formation; D000941:Antigens; D000961:Antilymphocyte Serum; D001402:B-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D007117:Immunization, Secondary; D007074:Immunoglobulin G; D007156:Immunologic Memory; D007700:Kinetics; D051379:Mice; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D013601:T-Lymphocytes / Q000276:immunology*
D000941:Antigens; D000961:Antilymphocyte Serum; D007074:Immunoglobulin G