title: Alterations in electrophysiology of isolated amphibian small intestine produced by removing the muscle layers.
abstract: Isolated segments of Amphiuma small intestine bathed in chloride or sulfate buffer generate a greater short-circuit current and a larger change in current in response to galactose when the serosal muscle layers are stripped from the mucosa. Intact (unstripped) segments are not apparently anoxic since stripped segments exposed to serosal N2 for 3 h display normal short-circuit currents but a reduced potential response to galactose, while the presence of muscle layers tends to reduce the short-circuit current but does not alter the potential response to galactose. Bullfrog small intestine also generates greater short-circuit current following removal of the muscle layers. The enhancing effect of stripping appears to be related to removal of a resistance to ion flow across the tissue.
mesh_terms: D000663:Amphibians; D000818:Animals; D004553:Electric Conductivity; D005690:Galactose / Q000494:pharmacology; D007413:Intestinal Mucosa / Q000502:physiology; D007421:Intestine, Small / Q000187:drug effects / Q000502:physiology*; D008564:Membrane Potentials / Q000187:drug effects; D009130:Muscle, Smooth / Q000502:physiology; D009584:Nitrogen / Q000494:pharmacology; D011892:Rana catesbeiana; D013997:Time Factors
chemical_list: D009584:Nitrogen; D005690:Galactose