title: Evidence that UV-inducible error-prone repair is absent in Haemophilus influenzae Rd, with a discussion of the relation to error-prone repair of alkylating-agent damage.
abstract: Haemophilus influenzae Rd and its derivatives are mutated either not at all or to only a very small extent by ultraviolet (UV) radiation, X-rays, methyl methanesulfonate, and nitrogen mustard, though they are readily mutated by such agents as N-methyl-N'-nitro-N-nitrosoguanidine, ethyl methanesulfonate, and nitrosocarbaryl. In these respects H. influenzae Rd resembles the lexA mutants of Escherichia coli that lack the SOS or reclex UV-inducible error-prone repair system. This similarity is further brought out by the observation that chloramphenicol has little or no effect on post-replication repair after UV irradiation. In E. coli, chloramphenicol has been reported to considerably inhibit post-replication repair in the wild type but not in the lexA mutant. Earlier work has suggested that most or all the mutations induced in H. influenzae by NC result from error-prone repair. Combined treatment with NC and either X-rays or UV shows that the NC error-prone repair system does not produce mutations from the lesions induced by these radiations even while it is producing them from its own lesions. It is concluded that the NC error-prone repair system or systems and the reclex error-prone system are different.
mesh_terms: D000477:Alkylating Agents / Q000494:pharmacology*; D004260:DNA Repair; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D006193:Haemophilus influenzae / Q000187:drug effects / Q000528:radiation effects; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D009153:Mutagens; D009154:Mutation; D009588:Nitrogen Mustard Compounds / Q000494:pharmacology; D009603:Nitroso Compounds / Q000494:pharmacology; D010641:Phenotype; D014466:Ultraviolet Rays; D014965:X-Rays
chemical_list: D000477:Alkylating Agents; D009153:Mutagens; D009588:Nitrogen Mustard Compounds; D009603:Nitroso Compounds; D008769:Methylnitronitrosoguanidine; D005020:Ethyl Methanesulfonate; D008741:Methyl Methanesulfonate