title: Evidence for a two-domain structure of the terminal membrane C5b-9 complex of human complement.
abstract: Lipid vesicles carrying the purified membrane C5b-9 complex [C5b-9(m)] of complement were analyzed immunochemically and in the electron microscope after treatment with a combination of trypsin and alpha-chymotrypsin. Under reducing conditions, the externally oriented annulus was removed. The remaining part of the C5b-9(m), representing approximately half of the total mass of the macromolecular complex, was visualized in the electron microscope as a hollow cylindrical structure with walls of 1-nm thickness. This structure remained tenaciously attached to the lipid bilayer, projecting 8-9 nm from the external membrane surface into the aqueous environment. Cleavage of C5b-9(m) by proteolysis and reduction resulted in a sharp reduction of tis antigenic determinants. One hydrophilic protease-resistant C5 derivative was released from the membrane and recovered in the fluid phase. The membrane-bound residue almost totally lacked antigens precipitable with antisera to C5, C6, C9, and C5b-9(m).
mesh_terms: D003182:Complement C5 / Q000378:metabolism; D003183:Complement C6 / Q000378:metabolism; D003184:Complement C7 / Q000378:metabolism; D003185:Complement C8 / Q000378:metabolism; D003186:Complement C9 / Q000378:metabolism; D003165:Complement System Proteins / Q000378:metabolism*; D003602:Cytotoxicity, Immunologic; D004229:Dithiothreitol / Q000494:pharmacology; D006801:Humans; D008051:Lipid Bilayers; D008563:Membrane Lipids / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000378:metabolism; D010713:Phosphatidylcholines
chemical_list: D003182:Complement C5; D003183:Complement C6; D003184:Complement C7; D003185:Complement C8; D003186:Complement C9; D008051:Lipid Bilayers; D008563:Membrane Lipids; D010713:Phosphatidylcholines; D003165:Complement System Proteins; D010447:Peptide Hydrolases; D004229:Dithiothreitol