Induction of erythroid differentiation in murine erythroleukemia cells by N-substituted polymethylene diamides.
Various N-substituted polymethylene diamides were synthesized and tested for their potency to induce erythroid differentiation in murine erythroleukemia cells. N,N,N',N'-tetramethyl-1.6-hexane-dicarboxamide (IIc) was the most potent inducer among 15 compounds tested. The effectiveness of this compound was similar to that of hexamethylene bisacetamide (HMBA). HMBA has a different amide linkage order from that of IIc. HMBA and IIc at a concentration of 5 mM had similar effects on the cell growth rate and induced a similar frequency of benzidine-positive cells. However, hemoglobin production was 1.5 times more effective with IIc. Polymethylene diester, diamide, dihydrazide and dianilide had no effect on the induction of hemoglobin synthesis. The N-alkylated amide group appears to be required for induction of differentiation in murine erythroleukemia cells.
D000577:Amides / Q000138:chemical synthesis / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D004912:Erythrocytes / Q000187:drug effects*; D006454:Hemoglobins / Q000096:biosynthesis; D004915:Leukemia, Erythroblastic, Acute / Q000097:blood*; D007942:Leukemia, Experimental / Q000097:blood; D051379:Mice
D000577:Amides; D006454:Hemoglobins