Effect of dietary alfalfa, pectin, and wheat bran on azoxymethane-or methylnitrosourea-induced colon carcinogenesis in F344 rats.
The effect of dietary alfalfa, pectin, and wheat bran on colon carcinogenesis was studied in female inbred F344 rats. Weanling rats were fed semipurified diets containing 0 or 15% alfalfa, pectin, or wheat bran. At 7 weeks of age, all animals except controls were given azoxymethane (AOM) sc at a dose rate of 8 mg/kg body weight/week for 10 weeks or methylnitrosourea (MNU) intrarectally at a dose rate of 2 mg/rat twice a week for 3 weeks. The AOM-treated group was autopsied 40 weeks and the MNU-treated group 30 weeks after the first injection of the carcinogen. No tumors were observed in the colon or other organs of untreated rats fed the various diets. The animals fed the alfalfa diet and treated with MNU had a higher incidence of colon tumors than did those fed the control diet or the diets containing pectin or wheat bran. The incidence of MNU-induced colon tumors did not differ between the animals fed the control diet or the diets containing pectin or wheat bran. However, the incidence of AOM-induced colon tumors in rats fed diets containing pectin or wheat bran was lower than that in rats fed the control diet or the alfalfa diet. These results thus indicate that the effect of fiber in colon carcinogenesis depends on the type of fiber and, possibly, the fiber's mode of action.
D000818:Animals; D001397:Azoxymethane; D002482:Cellulose / Q000627:therapeutic use*; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000178:diet therapy* / Q000473:pathology; D004043:Dietary Fiber / Q000627:therapeutic use*; D005260:Female; D000455:Medicago sativa; D008770:Methylnitrosourea; D009374:Neoplasms, Experimental / Q000139:chemically induced / Q000178:diet therapy / Q000473:pathology; D010368:Pectins / Q000627:therapeutic use*; D051381:Rats; D011916:Rats, Inbred F344; D014908:Triticum
D004043:Dietary Fiber; D008770:Methylnitrosourea; D010368:Pectins; D002482:Cellulose; D001397:Azoxymethane