title: Human (HLA-A and HLA-B) and murine (H-2K and H-2D) histocompatibility antigens are cell surface receptors for Semliki Forest virus.
abstract: The proteins coded for by the HLA-A and HLA-B loci in man and the H-2K and H-2D loci in mice were identified as cell surface receptors for Semliki Forest virus. This conclusion is based on the following observations: (i) Water-soluble octamers of viral coat proteins inhibit the complement-dependent cytotoxicity of antibodies directed against H-2K and H-2D antigens in mouse cells. (ii) Isolated detergent-soluble HLA-A and HLA-B antigens reconstituted in lipid vesicles inhibit the binding of viral proteins to human cells (as do the water-soluble antigens to a lesser extent). (iii) Reconstituted HLA-A and HLA-B vesicles interact in solution with Semliki Forest virus (or with vesicles containing viral spike proteins), as demonstrated by coprecipitation with antisera. (iv) Complexes between viral spoke proteins and HLA-A and HLA-B antigens or H-2K and H-2D antigens can be isolated from the cell surface by utilizing affinity chromatography or immunoprecipitation.
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D006183:H-2 Antigens; D006680:HLA Antigens; D006801:Humans; D008565:Membrane Proteins / Q000276:immunology; D051379:Mice; D011991:Receptors, Virus; D012672:Semliki forest virus; D014764:Viral Proteins / Q000276:immunology
chemical_list: D006183:H-2 Antigens; D006680:HLA Antigens; D008565:Membrane Proteins; D011991:Receptors, Virus; D014764:Viral Proteins