Inability of choleragenoid-sensitized cells to induce T cell-mediated cytolysis.
Murine splenocytes and tumor cells bind cholera enterotoxoid (choleragenoid). Four hours after sensitization, choleragenoid-coated cells were lysed in the presence of anti-cholergenoid serum and complement, indicating that the binding was stable. Choleragenoid-coated cells were unable to sensitize spleen cells from normal or choleragenoid primed syngeneic mice into displaying a cytotoxic effect against choleragenoid-coated target cells in the T cell-mediated cytotoxicity assay. Cells coated with both choleragenoid and trinitrophenyl (TNP) groups did sensitize syngeneic spleen cells to display a cytotoxic effect against target cells bearing choleragenoid and TNP or TNP alone, but not choleragenoid alone. These data demonstrate that the mere binding of a foreign component to lymphoid cells is not sufficient to allow sensitization of cytotoxic T cells.
D000818:Animals; D000906:Antibodies; D001666:Binding Sites, Antibody; D002462:Cell Membrane / Q000276:immunology; D002771:Cholera / Q000276:immunology*; D003165:Complement System Proteins; D003601:Cytotoxicity Tests, Immunologic; D004768:Enterotoxins / Q000494:pharmacology*; D006649:Histocompatibility Antigens; D066298:In Vitro Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D009374:Neoplasms, Experimental / Q000276:immunology; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014301:Trinitrobenzenes / Q000276:immunology
D000906:Antibodies; D004768:Enterotoxins; D006649:Histocompatibility Antigens; D014301:Trinitrobenzenes; D003165:Complement System Proteins