Inhibition by interferon of thymidine uptake in chemostat cultures of L1210 cells.
Mouse interferon preparations inhibited the incorporation of [3H]-thymidine into acid-insoluble material of mouse leukemia L1210 cells cultivated under steady-state conditions in a chemostat. Interferon exerted only a transitory and less pronounced effect on [3H]-deoxyadenosine incorporation and had no effect on the content of total DNA per cell. Study of [3H]-thymidine uptake at 1 degree into acid-soluble cellular material showed that interferon reduced the uptake of this labeled nucleoside whereas the uptake of [3H]-deoxyadenosine and [3H]-deoxy-D-glucose was not inhibited. The effect of interferon on [3H]-thymidine uptake occurred prior to the inhibitory action on cell multiplication. It is suggested that the inhibitory of [3H]-thymdine uptake reflected specific changes in the cell membrane of interferon-treated cells which may be relevant to the understanding of the antiviral and other biological effects of interferon.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects; D004273:DNA, Neoplasm / Q000096:biosynthesis; D003839:Deoxyadenosines / Q000378:metabolism; D003847:Deoxyglucose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D007372:Interferons / Q000494:pharmacology*; D007939:Leukemia L1210; D051379:Mice; D013936:Thymidine / Q000378:metabolism*
D004273:DNA, Neoplasm; D003839:Deoxyadenosines; D007372:Interferons; D003847:Deoxyglucose; D005947:Glucose; D013936:Thymidine