title: Epidermal growth factor and the multiplication of cultured human epidermal keratinocytes.
abstract: The culture lifetime of epidermal cells of newborn humans is increased from 50 to 150 generations by adding to the medium epidermal growth factor, a polypeptide mitogen. EGF seems to delay senescence of the cells by maintaining them in a state further removed from terminal differentiation. This effect is revealed by a greater ability of the cells to survive subculture and initiate new colonies, but not necessarily by an increased growth rate.
mesh_terms: D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured / Q000187:drug effects*; D002999:Clone Cells / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D010455:Peptides / Q000494:pharmacology*; D012867:Skin / Q000166:cytology
chemical_list: D010455:Peptides; D004815:Epidermal Growth Factor