A rapid method for the preparation of high potency auto and alloantibody eluates.
The present report describes a modified, rapid acid-stromal method for elution of erythrocyte antibodies. With this method, potent crystal clear auto and alloantibody eluates can be produced. The simplicity of the method makes it readily adaptable to the routine serological laboratory or blood bank. In semiquantitative and quantitative studies, we have found that this method compares favorably with other antibody elution methods. With alloantibodies, the rapid acid elution method and the ether method produced the strongest eluates with the ether eluates being slightly more potent than the rapid eluated. By contrast, with autoantibodies the rapid method was clearly superior, producing much stronger IgG erythrocyte coating as judged by the 125I antiglobulin test.
D000906:Antibodies / Q000302:isolation & purification*; D000888:Antibodies, Anti-Idiotypic; D001323:Autoantibodies / Q000302:isolation & purification*; D001793:Blood Preservation; D003298:Coombs Test; D004072:Digitonin; D004912:Erythrocytes / Q000276:immunology; D005998:Glycine; D006801:Humans; D007074:Immunoglobulin G; D007158:Immunologic Techniques / Q000379:methods*
D000906:Antibodies; D000888:Antibodies, Anti-Idiotypic; D001323:Autoantibodies; D007074:Immunoglobulin G; D004072:Digitonin; D005998:Glycine