Drugs as ligands of immunogenic molecules in parasites: an approach to the isolation of target-antigens.
Schistosomicide drugs are used as ligands to isolate the target antigens of schistosome by affinity chromatography. Anti-target antigens immune sera produced in rabbit permit their localization on the parasite using immunofluorescence. Target antigens of some drugs determine a relatively high immunoprotection in rat or mouse schistosomiasis infection.
D000818:Animals; D000941:Antigens / Q000302:isolation & purification*; D002265:Carboxylic Ester Hydrolases / Q000032:analysis; D006224:Cricetinae; D003601:Cytotoxicity Tests, Immunologic; D005260:Female; D005455:Fluorescent Antibody Technique; D007109:Immunity / Q000187:drug effects*; D007112:Immunity, Maternally-Acquired; D007123:Immunoelectrophoresis, Two-Dimensional; D008099:Liver / Q000469:parasitology; D008297:Male; D051379:Mice; D010088:Oxidoreductases / Q000032:analysis; D011817:Rabbits; D051381:Rats; D011916:Rats, Inbred F344; D012550:Schistosoma mansoni / Q000276:immunology*; D012556:Schistosomicides / Q000494:pharmacology*
D000941:Antigens; D012556:Schistosomicides; D010088:Oxidoreductases; D002265:Carboxylic Ester Hydrolases