Effect of maleic anhydride-vinyl ether on the lymphocyte subpopulations of tumor-bearing, irradiated mice.
The splenic T- and B-lymphocyte populations of BALB/c mice were determined in Madison lung carcinoma 109-bearing animals. Concurrently, some groups of tumored mice were exposed to 500 rads of whole-body irradiation and were treated with one dose of maleic anhydride-vinyl ether (MVE). By direct immunofluorescence, it was found that the percentage of splenic T lymphocytes was significantly depressed in the tumored-irradiated mice. Mitogenic studies revealed that the T lymphocytes were more depressed in the tumored-irradiated mice than in the corresponding nonirradiated tumored mice. MVE was relatively effective in reconstituting the T-cell compartment of these splenic T lymphocytes. The B-cell compartment of the splenic lymphocytes of the tumored-irradiated mice was found to be extremely radiosensitive. Utilizing a specific anti-B serum, no B lymphocytes were detected during the testing. Blastogenic studies using lipopolysaccharide as the mitogenic probe revealed that the incorporation of 3H-TdR of the tumored-irradiated mice was just slightly higher than background values. MVE proved to be relatively ineffective in reconstituting the splenic B cells of the tumored-irradiated mice.
D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects*; D007958:Leukocyte Count; D008175:Lung Neoplasms / Q000097:blood / Q000276:immunology / Q000628:therapy*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000528:radiation effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009374:Neoplasms, Experimental / Q000628:therapy; D011108:Polymers / Q000494:pharmacology*; D011713:Pyran Copolymer / Q000494:pharmacology*; D011836:Radiation Tolerance; D013601:T-Lymphocytes / Q000187:drug effects*
D011108:Polymers; D011713:Pyran Copolymer