Double-osmotic-shock procedure to prepare gentamicin adenine mononucleotide transferase for the enzymatic assay of gentamicin.
The release of gentamicin adenine mononucleotide transferase during single, cold, osmotic-shock treatment of Escherichia coli K-12 W677/HJR66 is not always maximal. The quantity of gentamicin adenine mononucleotide transferase in osmotic extracts could be significantly increased when a double-shock procedure was performed.
D000249:Adenosine Monophosphate / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology; D005839:Gentamicins / Q000032:analysis* / Q000378:metabolism; D009713:Nucleotidyltransferases / Q000302:isolation & purification*; D009997:Osmotic Pressure
D005839:Gentamicins; D000249:Adenosine Monophosphate; D009713:Nucleotidyltransferases