Human chronic lymphocytic leukaemia. Surface markers and activation of lymphocytes.
Cell surface markers and the responses of lymphocytes to T- and B-cell mitogens were studied in 10 patients with CCL. T cells were identified as cells rosetting with sheep red blood cells (SRBC), and S-Ig was used as a marker for B lymphocytes. Most cells from all patients had a detectable amounts of S-Ig, and the percentage of cells rosetting with SRBC was low in all cases. Of the lymphocytes from these patients, 3-74% (mean 33%) were positive for the acid esterase (ANAE), which has been claimed to be a T-cell marker. However, some patients had cells that were positive for both S-Ig and ANAE. Acid esterase staining is therefore not a valid T-cell marker in chronic lymphocytic leukaemia. In cultures containing the T-cell mitogen leucoagglutinin (LA) and the T- and B-cell mitogen pokeweed mitogen (PWM) the reactivity of the lymphocytes was low. The cells responded vigorously to the T- and B-cell mitogen protein A (PA); however, the response was serum-dependent, being strong in a culture medium containing foetal calf serum (FCS), but impaired in the presence of human AB serum. Only 1 patient had cells that responded to the B-cell mitogen LPS.
D000368:Aged; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D002478:Cells, Cultured; D002908:Chronic Disease; D004950:Esterases / Q000097:blood; D005260:Female; D006801:Humans; D007945:Leukemia, Lymphoid / Q000201:enzymology / Q000276:immunology*; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D008934:Mitogens / Q000494:pharmacology; D011947:Receptors, Antigen, B-Cell / Q000032:analysis*; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000187:drug effects / Q000201:enzymology / Q000276:immunology*
D008934:Mitogens; D011947:Receptors, Antigen, B-Cell; D004950:Esterases