title: A chemical approach to the mechanism of B-lymphocyte activation. II. The pure presentation of haptens does not inactivate B lymphocytes.
abstract: Dinitrophenyl (DNP)-lysine-polymethylmethacrylate and DNP-cellulose conjugates do not irreversibly inactivate anti-DNP antigen-sensitive cells, regardless of the dose (up to 10 mg) or persistence of the stimulation (up to 2 weeks). Since these conjugates constitute pure hapten presentations, it is concluded that the pure hapten presentation to B lymphocyte does not irreversibly inactivate them. When murine spleen cells are cultured with Escherichia coli lipopolysaccharide (LPS) and (non-immunogenic) DNP-lysine-polymethylmethacrylate or (non-immunogenic)  DNP-cellulose conjugates, an anti-DNP immune response occurs. However, replacement of DNP-lysine-polymethylmethacrylate with polymethylmethacrylate, or DNP-cellulose with cellulose, also results in a similar anti-DNP response. It is consequently concluded that the anti-DNP responses are entirely elicited by LPS, the hapten Dnp being inoperative. The anti-DNP response elicited by DNP-Ficoll is, upon exhaustive testing, carrier-dependent. This implies that the mechanism of DNP-Ficoll immunogenicity is not two cooperative signals passed on to B lymphocytes via the hapten DNP. These results argue against any two-signal model of B-lymphocyte activation.
mesh_terms: D000180:Acrylic Resins / Q000494:pharmacology*; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D001402:B-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D002482:Cellulose / Q000276:immunology / Q000494:pharmacology; D005362:Ficoll / Q000276:immunology / Q000494:pharmacology; D006241:Haptens; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D008815:Mice, Inbred Strains; D009578:Nitrobenzenes / Q000494:pharmacology*; D011109:Polymethacrylic Acids / Q000276:immunology / Q000494:pharmacology*; D014301:Trinitrobenzenes / Q000276:immunology / Q000494:pharmacology*
chemical_list: D000180:Acrylic Resins; D006241:Haptens; D008070:Lipopolysaccharides; D009578:Nitrobenzenes; D011109:Polymethacrylic Acids; D014301:Trinitrobenzenes; D005362:Ficoll; D002482:Cellulose