Some factors affecting the production, by cultured baby-hamster kidney cells, of BHK glycoprotein I which cross-reacts immunologically with Tamm-Horsfall glycoprotein.
Cultured baby-hamster kidney cells (BHK-21/C13), which are adapted to grow in suspension (strain 2P), roduce a glycoprotein, termed BHK glycoprotein I, which cross-reacts immunologically with hamster urinary (Tamm-Horsfall glycoprotein. BHK glycoprotein I was isolated in an electrophoretically (sodium dodecyl sulphate/polyacrylamide gel) homogeneous form by application of affinity chromatography to the medium in which cells had been cultured. Insolubilized anti-(Tamm-Horsfall glycoprotein immunoglobulin G) was used as the adsorbent. The amount of BHK glycoprotein I associated with the cultured cells was found by both radioimmunoassay and immunofluorescence to be related to the amount of Ca2+ in the medium and to the particular stage of the cell cycle. 5'-Nucleotidase was also shed by the cells into the culture medium in amounts related to the stage of the cell cycle. The turnover of hamster Tamm-Horsfall glycoprotein in vivo appeared to be considerably more rapid than can be accounted for by cell turnover. Hamster Tamm-Horsfall glycoprotein was shown to be ineffective in inhibiting agglutination of chicken erythrocytes caused by influenza virus.
D002118:Calcium; D002460:Cell Line; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D003429:Cross Reactions; D003470:Culture Media; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000096:biosynthesis* / Q000276:immunology / Q000652:urine; D006387:Hemagglutination, Viral; D008938:Mitosis; D009708:Nucleotidases / Q000378:metabolism; D011863:Radioimmunoassay; D012709:Serum Albumin / Q000378:metabolism; D012967:Sodium Dodecyl Sulfate
D003470:Culture Media; D006023:Glycoproteins; D012709:Serum Albumin; D012967:Sodium Dodecyl Sulfate; D009708:Nucleotidases; D002118:Calcium