[Isolation of purified populations of T- and B-lymphocytes under clinical conditions].
The authors present comparative characteristics of the methods of isolation of T- and B-lymphocytes in patients with chronic renal insufficiency. T-lymphocytes were obtained on columns with neilon cotton wool. B-cells were fractionated by repeated centrifugation in the gradient of phycol-urotrast density after staging E-rosette formation with the total lymphocyte pool. Methodical peculiarities of single-stage and two-stage B-lymphocyte fractionation were revealed.
D000328:Adult; D000906:Antibodies; D001402:B-Lymphocytes; D002469:Cell Separation / Q000379:methods; D002499:Centrifugation, Density Gradient; D003165:Complement System Proteins; D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000276:immunology*; D008297:Male; D012397:Rosette Formation; D013601:T-Lymphocytes
D000906:Antibodies; D003165:Complement System Proteins
Vydelenie ochishchennykh populiatsiĭ T- i B-limfotsitov v klinicheskikh usloviiakh.