alpha-Amylase from human pancreatic juice as an electrophoretically pure isozyme.
Human pancreatic juice collected antiseptically and strictly free from other body fluids and juices, was examined for the isozyme composition of alpha-amylase. Unlike those of the extract of and exudate from pancreatic tissue, the composition of alpha-amylase isozyme in the secreted juice was simple and consisted of only two isozymes, a major one and a minor one, and the reason for difference in the isozyme composition is discussed. The major alpha-amylase isozyme was obtained in a crystalline state with a molecular weight of 5.4-5.5 X 10(4).
D000681:Amylases / Q000302:isolation & purification*; D007527:Isoenzymes / Q000302:isolation & purification*; D010189:Pancreatic Juice / Q000201:enzymology*; D000516:alpha-Amylases / Q000302:isolation & purification*
D007527:Isoenzymes; D000681:Amylases; D000516:alpha-Amylases