Partial characterization of colony stimulating activity from human leucocyte conditioned medium tested on human marrow cells.
Conditioned medium from normal human leucocytes was fractionated by gel filtration (Sephadex G 150) and affinity chromatography (Con A-Sepharose). On gel filtration, all colony stimulating activity (CSA) was eluted as a single peak with a molecular weight of 33.000 -- 41.000. Affinity chromatography revealed two separate peaks of colony stimulating activity, one lacking and the other having concanavalin A affinity (MW congruent to 44.000 -- 58.000). The properties of these two fractions are under investigation.
D001854:Bone Marrow Cells; D055598:Chemical Phenomena; D002621:Chemistry; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D003115:Colony-Stimulating Factors / Q000302:isolation & purification* / Q000502:physiology; D006801:Humans; D007962:Leukocytes / Q000032:analysis*; D008970:Molecular Weight
D003115:Colony-Stimulating Factors