Generation of factor VIII coagulant activity by isolated, perfused neonatal pig livers and adult rat livers.
Isolated, normal, neonatal pig livers released VIII:C into perfusates of porcine von Willebrand blood lacking VIIIR:AG and VIII:RWF. Neonatal von Willebrand livers released VIII:C only when partially purified VIIIR:AG was added to the von Willbrand blood. Adult rat livers, perfused with frozen-thawed erythrocytes suspended in Tyrode's solution containing 6% bovine serum albumin and hence no VIIIR:AG released VIII:C only when serum, adsorbed serum or cryoprecipitate from rat plasma was added to the perfusate. Cycloheximide blocked the release of VIII:C from rat liver.
D000818:Animals; D000831:Animals, Newborn; D001780:Blood Coagulation Tests; D005169:Factor VIII / Q000378:metabolism*; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism*; D010477:Perfusion; D051381:Rats; D013552:Swine; D014842:von Willebrand Diseases / Q000097:blood
D005169:Factor VIII