Split-dose and liquid-holding recovery after X-irradiation on diploid yeast Saccharomyces cerevisiae. I. Dependence on growth-phase.
Liquid-holding recovery (LHR) and the sparing effect of dose fractionation were investigated with 100 kVp-X-rays in diploid yeast from exponential and stationary phase. Exponential cells showed prompt and complete split-dose recovery. The ability to undergo LHR remained constant during 2-5 hours incubation after the first dose. Stationary-phase cells did not exhibit split-dose recovery (SDR) in a simple way: there was an increase in sensitivity after 3-5 hours followed by a gradual increases with time. It is suggested that stationary cells are starved of an essential co-factor which has to be synthesized de nova.
D004260:DNA Repair; D004307:Dose-Response Relationship, Radiation; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000528:radiation effects*; D014965:X-Rays