title: Protein synthesis and secretion by activated human lymphocytes.
abstract: Human peripheral blood mononuclear cells showed increased synthesis and secretion of protein in response to stimulation by mitogens. Protein secretion in response to concanavalin A (Con A) correlated with cellular proliferation and originated from the proliferating cells. Selective depletion of either B lymphocytes or monocytes did not lead to a reduction in the total protein secreted, suggesting that the T lymphocyte was the cell contributing most of the synthetic activity. It is concluded that the measurement of newly synthesized and secreted protein by Con A activated mononuclear cells is a measurement of the T-lymphocyte response.
mesh_terms: D000361:Agammaglobulinemia / Q000378:metabolism; D003208:Concanavalin A / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D006801:Humans; D007700:Kinetics; D037102:Lectins / Q000494:pharmacology; D008213:Lymphocyte Activation; D008937:Mitomycins / Q000494:pharmacology; D011506:Proteins / Q000378:metabolism*; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
chemical_list: D037102:Lectins; D008937:Mitomycins; D011506:Proteins; D003208:Concanavalin A; D004247:DNA