Effects of various periods of food deprivation on serotonin synthesis in the lateral hypothalamus.
One hr following an infusion of 3H-L-tryptophan, the lateral hypothalamus was perfused with physiological bacteriostatic saline for 40 min. Samples of perfusate, which corresponded to 75--90 min post-infusion, were analyzed by thin layer chromatography for estimation of 3H-labelled L-tryptophan, 5-hydroxytryptophan and 5-hydroxytryptamine. The results indicate that tryptophan uptake and serotonin synthesis are enhanced as a function of hours of food deprivation.
D006916:5-Hydroxytryptophan / Q000378:metabolism; D000818:Animals; D002855:Chromatography, Thin Layer; D005508:Food Deprivation; D007031:Hypothalamus / Q000033:anatomy & histology / Q000378:metabolism*; D008297:Male; D051381:Rats; D012701:Serotonin / Q000096:biosynthesis*; D013997:Time Factors; D014364:Tryptophan / Q000378:metabolism
D012701:Serotonin; D014364:Tryptophan; D006916:5-Hydroxytryptophan