Identification of T and B lymphocytes in pigs by combined E-rosette test and surface Ig labelling.
A procedure for combining labelling of B lymphocytes with [125I]anti-Ig-antibodies and E-rosetting is described which permits simultaneous detection of B lymphocytes and T lymphocytes. Pig lymphocytes were incubated with iodinated anti-Ig antibodies and the E-rosette test with SRBC was performed. E-rosettes appeared stable after methanol fixation of cell smears on agarose-coated slides and treatment for autoradiography on stripping films. The percentage of cells classified as B or T lymphocytes in pig peripheral blood closely resembles that in man. No doubly marked cells were demonstrated provided cells were classified as E-rosettes only when 5 or more red cells were attached.
D000818:Animals; D000831:Animals, Newborn; D001345:Autoradiography; D001402:B-Lymphocytes / Q000276:immunology*; D002499:Centrifugation, Density Gradient; D004912:Erythrocytes / Q000276:immunology; D007106:Immune Sera / Q000494:pharmacology; D011947:Receptors, Antigen, B-Cell; D012397:Rosette Formation; D012756:Sheep; D013552:Swine; D013601:T-Lymphocytes / Q000276:immunology*
D007106:Immune Sera; D011947:Receptors, Antigen, B-Cell