title: The solubilization of bone and dentin collagens by pepsin. Effect of cross-linkages and non-collagen components.
abstract: Bone and dentin collagen are less susceptible to solubilization by pepsin digestion then is skin collagen. Digestion at 4 degrees C for 72 h solubilized only 35.3% of bovine cortical bone and 5.6% of bovine dentin compared with nearly 100% dissolution of bovine skin. Sodium dodecyl sulfate-acrylamide gel electrophoresis and molecular sieve chromatography showed that, for bone and dentin, intact alpha chains and cross-linked aggregates of beta, gamma and higher weight remained intact after pepsin solubilization but lower molecular weight fragments also were prevalent indicating chain scission in helical regions. Electron microscopic examination of segment long spacing precipitates of the soluble collagens confirmed the presence of solubilized polymerized collagen. The principal reducible cross-link in both bone and dentin was the precursor of dihydroxylsinonorleucine and this cross-link was also present in the solubilized collagens. Small amounts of non-collagenous proteins and glycosaminoglycans of different compositions in dentin and bone resisted extraction before pepsin digestion. However, the differences in solubilization of the collagens have been related to differences in cross-linkage placement.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001842:Bone and Bones / Q000032:analysis* / Q000648:ultrastructure; D002417:Cattle; D003094:Collagen / Q000302:isolation & purification; D003804:Dentin / Q000032:analysis*; D004589:Electrophoresis, Disc; D006595:Hexosamines / Q000032:analysis; D008239:Lysine; D046911:Macromolecular Substances; D008854:Microscopy, Electron; D008963:Molar; D008970:Molecular Weight; D009928:Organ Specificity; D010434:Pepsin A; D011487:Protein Conformation; D012995:Solubility; D014574:Uronic Acids / Q000032:analysis
chemical_list: D000596:Amino Acids; D006595:Hexosamines; D046911:Macromolecular Substances; D014574:Uronic Acids; D003094:Collagen; D010434:Pepsin A; D008239:Lysine