In vitro specular microscope perfusion of M-K- and moist chamber-stored human corneas.
Human donor corneas were stored in McCarey-Kaufman (M-K) medium for 3 to 6 days; then they were mounted in the specular microscope, and the endothelial surface perfused with a glutathione-bicarbonate-Ringer solution. During storage, the corneal thickness increased 37% above an assumed normal of 0.520 mm. The corneas did not have sufficient active thinning to be accepted as viable when tested by the temperature-reversal phenomenon. Since comparable donor corneas have been used in successful penetrating keratoplasties, there must be a discrepancy between the cell viability as tested by the temperature-reversal phenomenon and clinical application.
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D002415:Cats; D002470:Cell Survival; D003315:Cornea / Q000166:cytology*; D016039:Corneal Transplantation; D004727:Endothelium / Q000166:cytology; D005125:Eye Banks; D006801:Humans; D066298:In Vitro Techniques; D008853:Microscopy; D008875:Middle Aged; D009926:Organ Preservation / Q000379:methods*; D010477:Perfusion; D011817:Rabbits; D012996:Solutions; D014021:Tissue Preservation / Q000379:methods*; D014022:Tissue Survival
D012996:Solutions