title: Quantitative determination of free amino acids by gas-liquid chromatography with special reference to yeasts.
abstract: A gas-liquid chromatographic method for quantitative determination of free amino acids in yeasts is described. The amino acids are extracted with boiling water and separated from interfering substances on a cation-exchange resin. They are analyzed as their n-propyl N-acetyl esters. The best separation of the derivatives was achieved when using glass columns containing a mixture of three polar phases.
mesh_terms: D000596:Amino Acids / Q000032:analysis*; D002849:Chromatography, Gas; D002853:Chromatography, Liquid; D012441:Saccharomyces cerevisiae / Q000032:analysis; D004718:Saccharomycetales / Q000032:analysis; D015003:Yeasts / Q000032:analysis*
chemical_list: D000596:Amino Acids