Rat insulin genes: construction of plasmids containing the coding sequences.
Recombinant bacterial plasmids have been constructed that contain complementary DNA prepared from rat islets of Langerhans messenger RNA. Three plasmids contain cloned sequences representing the complete coding region of rat proinsulin I, part of the preproinsulin I prepeptide, and the untranslated 3' terminal region of the mRNA. A fourth plasmid contains sequences derived from the A chain region of rat preproinsulin II.
D000818:Animals; D001483:Base Sequence; D003062:Codon; D004247:DNA / Q000302:isolation & purification; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004269:DNA, Bacterial / Q000032:analysis / Q000378:metabolism; D004274:DNA, Recombinant / Q000032:analysis / Q000378:metabolism; D004926:Escherichia coli; D005111:Extrachromosomal Inheritance; D005796:Genes; D007515:Islets of Langerhans / Q000032:analysis; D010957:Plasmids; D011384:Proinsulin / Q000096:biosynthesis; D011498:Protein Precursors / Q000096:biosynthesis; D012333:RNA, Messenger / Q000302:isolation & purification; D051381:Rats; D014170:Transformation, Genetic
D003062:Codon; D004269:DNA, Bacterial; D004274:DNA, Recombinant; D011498:Protein Precursors; D012333:RNA, Messenger; D004247:DNA; D011384:Proinsulin; D004262:DNA Restriction Enzymes