Implication of tryptophan and tyrosine in the binding of p-nitrophenyl-alpha-D-mannopyranoside by concanavalin A. A radiolytic study.
Equilibrium dialysis has been used to study the effects of 60Co gamma-radiolysis on the carbohydrate binding site of Con A. Reaction of eaq- and OH. with Con A is accompanied by a decrease in the number of binding sites. Modification of the binding site by (Br)2- is independent of the initial concentration of Br-. Reaction of (SCN)2- with the protein, however, is dependent on the initial SCN- concentration. The results imply that tryptophan and possibly tyrosine are probably involved in the carbohydrate binding process.
D001665:Binding Sites / Q000528:radiation effects; D003208:Concanavalin A / Q000378:metabolism*; D003956:Dialysis / Q000379:methods; D005720:Gamma Rays; D006027:Glycosides / Q000378:metabolism*; D008362:Mannosides / Q000378:metabolism*; D011485:Protein Binding / Q000528:radiation effects; D014364:Tryptophan / Q000378:metabolism*; D014443:Tyrosine / Q000378:metabolism*
D006027:Glycosides; D008362:Mannosides; D003208:Concanavalin A; D014443:Tyrosine; D014364:Tryptophan