The influence of fixation on immunoperoxidase staining of plasmacells in paraffin sections of intestinal biopsy specimens.
The influence of different fixation methods on the results of immunoperoxydase staining of immunoglobulin and gastrin producing cells in gastric and duodenal mucosa was investigated. An indirect method was used on paraffin sections. It appeared that that fixatives containing sublimate gave the most consistent results, a sublimate-formaldehyde mixture being the best.
D005404:Fixatives / Q000494:pharmacology*; D005557:Formaldehyde / Q000494:pharmacology; D005753:Gastric Mucosa / Q000166:cytology*; D005755:Gastrins; D006801:Humans; D007124:Immunoenzyme Techniques; D007413:Intestinal Mucosa / Q000166:cytology*; D010950:Plasma Cells / Q000032:analysis* / Q000187:drug effects
D005404:Fixatives; D005755:Gastrins; D005557:Formaldehyde