Stimulation of platelet production in vivo by a factor in cell culture conditioned medium.
Injection of medium conditioned by a murine myelomonocytic leukaemic cell-line (WEHI-CM) stimulates platelet production in irradiated, bone marrow reconstituted mice. Media conditioned by the growth of normal bone marrow cells (BM-CM) or by a lymphoid leukaemia cell line had no effect on platelet production. However, the effect of WEHI-CM on platelet production was further enhanced when injected along with BM-CM, indicating that more than one factor may play a role in the regulation of megakaryocytopoiesis.
D000818:Animals; D001792:Blood Platelets / Q000187:drug effects*; D001854:Bone Marrow Cells; D002460:Cell Line; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D003115:Colony-Stimulating Factors / Q000494:pharmacology*; D005260:Female; D007951:Leukemia, Myeloid; D051379:Mice
D003115:Colony-Stimulating Factors