The ontogeny of thymic independent antibody responses in vitro in normal mice and mice with an X-linked B cell defect.
The primary in vitro antibody response of neonatal spleen cells to three thymic independent antigens has been examined. The time of onset of responsiveness to TNP-Brucella abortus and TNP-lipopolysaccharide was significantly earlier than the onset of responsiveness to TNP-Ficoll. This ontologic sequence was not affected by T cell depletion or antigen presentation on adult macrophages. In neonatal mice bearing the X-linked CBA/N defect, the response to TNP-Brucella abortus and TNP-lipopolysaccharide was much delayed and no response to TNP-Ficoll developed. We conclude that different thymic independent antigens address different subpopulations of B cells, one of which appears earlier in ontogeny than the other.
D000818:Animals; D000917:Antibody Formation; D000941:Antigens; D001402:B-Lymphocytes / Q000276:immunology*; D005260:Female; D005362:Ficoll / Q000276:immunology; D006462:Hemolytic Plaque Technique; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D008811:Mice, Inbred DBA; D012730:Sex Chromosomes; D013601:T-Lymphocytes / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology*; D014301:Trinitrobenzenes / Q000276:immunology; D014960:X Chromosome
D000941:Antigens; D014301:Trinitrobenzenes; D005362:Ficoll