Development and evaluation of an enzyme-labeled antibody test for the rapid detection of hog cholera antibodies.
A rapid enzyme-labeled antibody (ELA) microtechnique for the screening of swine for hog cholera antibodies was developed and evaluated with a blind study, using a 640-sample hog cholera serum bank. The total time to run a group of 22 samples was approximately 1 hour. The ELA test results correlated greater than 99% with hog cholera serum-neutralization test results on the same serums. Test results also indicated that the ELA test shares with the hog cholera serum-neutralization test the problem of cross reactions between the antibodies of hog cholera and bovine viral diarrhea.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D006691:Classical Swine Fever / Q000175:diagnosis* / Q000276:immunology; D003429:Cross Reactions; D007124:Immunoenzyme Techniques; D012328:RNA Viruses / Q000276:immunology*; D013552:Swine
D000914:Antibodies, Viral