Ultraviolet induction of prophage lambda during inhibition of deoxyribonucleic acid synthesis by hydroxyurea.
Hydroxyurea inhibited synthesis of certain deoxyribonucleic acid (DNA) precursors and causes the cessation of DNA synthesis. It did not cause induction of lambda. Superinfection of an irradiated lysogen with lambdaind- could prevent induction, but the percentage of cells protected decreased as the time between irradiation and superinfection increased. The presence of hydroxyurea did not increase the time during which cells could be rescued by superinfection. The accumulation of DNA precursors after ultraviolet or ionizing radiation was not necessary for the induction of lambda prophage to occur.
D003037:Cobalt Radioisotopes; D003090:Coliphages / Q000254:growth & development*; D004269:DNA, Bacterial / Q000096:biosynthesis*; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D006918:Hydroxyurea / Q000494:pharmacology*; D008242:Lysogeny / Q000187:drug effects; D014466:Ultraviolet Rays; D014779:Virus Replication
D003037:Cobalt Radioisotopes; D004269:DNA, Bacterial; D006918:Hydroxyurea