Diphtheria toxin-binding glycoproteins on hamster cells: candidates for diphtheria toxin receptors.
Diphtheria toxin-binding glycoproteins of high molecular weight (greater than 100,000) were identified on the surface of lymph node and thymus cells from hamsters, a diphtheria toxin-sensitive species. These diphtheria toxin-binding glycoproteins also interacted with CRM197 protein, which possesses toxin-blocking activity, but not with diphtheria toxoid, fragment A of diphtheria toxin, or cholera toxin, all of which lack toxin-blocking activity. These observations are consistent with the hypothesis that the detected diphtheria toxin-binding glycoproteins are involved in intoxication of cells by this toxin and possibly serve as the plasma membrane receptors for diphtheria toxin.
D000818:Animals; D002352:Carrier Proteins / Q000032:analysis / Q000378:metabolism*; D006224:Cricetinae; D004167:Diphtheria Toxin / Q000378:metabolism*; D005260:Female; D006023:Glycoproteins / Q000032:analysis / Q000378:metabolism*; D008214:Lymphocytes / Q000378:metabolism*; D008565:Membrane Proteins / Q000032:analysis / Q000378:metabolism*; D008970:Molecular Weight; D013601:T-Lymphocytes / Q000378:metabolism
D002352:Carrier Proteins; D004167:Diphtheria Toxin; D006023:Glycoproteins; D008565:Membrane Proteins