A squash technique for studying the cytology of maize endosperm and other tissues.
A new cytological procedure specifically suited to maize endosperms is presented. It uses 8-hydroxyquinoline with sucrose and aeration to pretreat the tissues. Glusulase is used to spread the cells. The procedure makes it possible to squash endosperms into a single cell layer and to photograph as many as 70 chromosomes in the same focal plane. It also allows identification of translocation chromosomes. With a slight modification the technique has been applied successfully to root tips and other tissues.
D002875:Chromosomes / Q000648:ultrastructure*; D003584:Cytological Techniques; D006912:Hydroxyquinolines; D007621:Karyotyping; D014178:Translocation, Genetic; D003313:Zea mays / Q000648:ultrastructure*
D006912:Hydroxyquinolines