title: Intestinal peroxisomes of goldfish (Carassius auratus)--examination for hydrolase, dehydrogenase and carnitine acetyltransferase activities.
abstract: 1. Rate sedimentation and isopycnic centrifugation were used to analyse the subcellular sites of enzymes in homogenates of goldfish intestinal mucosa. 2. The results allowed the following allocations to be made: carnitine acetyl transferase-mitochondrial and peroxisomal, xanthine dehydrogenase and NAD: alpha-glycerophosphate dehydrogenase soluble phase, NADP: isocitrate dehydrogenase soluble phase and mitochondrial, and 2-naphthyl laurate hydrolase microsomal and/or brush border. 3. Histochemistry confirmed the use of alkaline phosphatase and 1-naphthyl acetate esterase as brush border and microsome markers respectively. 4. Urate oxidase, allantoinase, allantoicase, xanthine oxidase and glycollate/lactate oxidase, activities were undetectable, and 1-naphthyl palmitate hydrolase was present only as a contaminant from pancreas.
mesh_terms: D000123:Acetyltransferases / Q000378:metabolism*; D000818:Animals; D002332:Carnitine O-Acetyltransferase / Q000378:metabolism*; D002499:Centrifugation, Density Gradient; D003530:Cyprinidae / Q000378:metabolism*; D006054:Goldfish / Q000378:metabolism*; D006651:Histocytochemistry; D006867:Hydrolases / Q000378:metabolism*; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000033:anatomy & histology / Q000201:enzymology* / Q000648:ultrastructure; D008830:Microbodies / Q000201:enzymology*; D010088:Oxidoreductases / Q000378:metabolism*
chemical_list: D010088:Oxidoreductases; D000123:Acetyltransferases; D002332:Carnitine O-Acetyltransferase; D006867:Hydrolases