[Proton translocation in membranes of submitochondrial particles].
Effect of an electrophilous inhibitor, chlorophenacyl, on energy-dependent functions of submitochondrial particles is studied. Chlorophenacyl at concentrations up to 1 mM is found practically not to affect the generation of membrane potential under NADH and succinate oxidation and ATP hydrolysis and to be a strong inhibitor of oxidative phosphorylation and reverse electron transport. The mechanism of the inhibition of energy-dependent functions of submitochondrial particles with chlorophenacyl is different from that of electron transport inhibitor, energy transport inhibitors and classical uncoupling agents--protonophors. The data obtained are suggested to be due to the existence of two ways of proton translocation in submitochondrial particle membrane, phosphorylating and non-phosphorylating, the effect of chlorophenacyl being directed on phosphorylating way only.
D001692:Biological Transport / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004579:Electron Transport / Q000187:drug effects; D004735:Energy Transfer / Q000187:drug effects; D008564:Membrane Potentials / Q000187:drug effects; D008566:Membranes / Q000502:physiology; D008931:Mitochondria, Muscle / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism; D010085:Oxidative Phosphorylation / Q000187:drug effects; D011522:Protons; D014475:Uncoupling Agents / Q000494:pharmacology; D002721:omega-Chloroacetophenone / Q000494:pharmacology*
D011522:Protons; D014475:Uncoupling Agents; D002721:omega-Chloroacetophenone
Translokatsiia protona v membranakh submitokhondrial'nykh chastits.