Analyses of oligosaccharides by tagging the reducing end with a fluorescent compound. I. Application to glycoproteins.
The reducing end sugar of an oligosaccharide and 2-aminopyridine were linked by means of reductive amination with sodium cyanoborohydride. The fluorescent derivative of the oligosaccharide thus obtained, which had a positive charge, was subjected to two-dimensional paper electrophoresis. In the first direction, the sugar derivative moved according to its degree of polymerization, and in the second direction, it moved according to the structure of the borate complex. In this way fluorescent derivatives of saccharides were mapped on a sheet of paper. The method was applied to some known mono- and oligosaccharides and to the saccharides obtained by nitrous deamination of the oligosaccharide portions of glycoproteins (fetuin, Take-amylase A, and ovalbumin). The fingerprints thus obtained were characteristic of the chemical structures of the original oligosaccharides.
D000631:Aminopyridines; D004590:Electrophoresis, Paper; D005456:Fluorescent Dyes; D006023:Glycoproteins; D009844:Oligosaccharides / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D013329:Structure-Activity Relationship; D000516:alpha-Amylases
D000631:Aminopyridines; D005456:Fluorescent Dyes; D006023:Glycoproteins; D009844:Oligosaccharides; D000516:alpha-Amylases