Metabolic carbohydrate-labelling of glycolipids from mouse splenocytes. Mitogen-stimulated B and T cells show different labelling patterns.
Splenic lymphocytes from CBA/J, AKR/A/J, BALB/c/A, C57/BL/6J, C3H/HeJ and C3H/Tif nu/nu mice and B lymphocyte or T lymphocyte preparations derived from CBA/J mouse spleen were cultivated in the presence of either concanavalin A, phytohemagglutinin, Salmonella minnesota R595 lipopolysaccharide or Proteus mirabilis soluble lipoprotein. The mitogens stimulated the incorporation of [14C]galactose into acid-insoluble cell material with the same specificity for B or T cells as that known for thymidine incorporation. The glycolipids extracted from mitogen-activated, carbohydrate-labelled B or T cells were compared by thin-layer chromatography and characteristic differences between B and T cells were noted in the ganglioside as well as in the neutral glycolipid fractions. In addition, subsets of B or T cells, namely lipopolysaccharide-responsive or lipoprotein-responsive B-cell populations or nylon-purified T cells may be recognized by characteristic neutral glycolipid bands.
D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D003208:Concanavalin A; D006017:Glycolipids / Q000096:biosynthesis*; D037102:Lectins; D008070:Lipopolysaccharides / Q000494:pharmacology; D008074:Lipoproteins / Q000494:pharmacology; D008213:Lymphocyte Activation; D051379:Mice; D008815:Mice, Inbred Strains; D008934:Mitogens; D013601:T-Lymphocytes / Q000187:drug effects / Q000378:metabolism*
D006017:Glycolipids; D037102:Lectins; D008070:Lipopolysaccharides; D008074:Lipoproteins; D008934:Mitogens; D003208:Concanavalin A