Alteration of the erythrocyte membrane during Newcastle disease virus-induced hemolysis.
In attempting to explain the mechanism whereby hemoglobin is released during NDV-induced hemolysis it was found that marker substances like albumin or myoglobin with diameters larger than 30 A are retained in the erythrocyte membrane, whereas low molecular weight material is released. This indicates that NDV-induced hemolysis does not result in the formation of membrane lesions but rather in the alteration of membrane permeability. A prerequisite for this process is the insertion of viral envelope components into the cell membrane. NDV-induced hemolysis can be enhanced by virus-specific antibodies and complement. In this case the release of marker substances is due to membrane lesions since large molecules also are released from the erythrocytes.
D000818:Animals; D007611:Aprotinin / Q000378:metabolism; D002463:Cell Membrane Permeability; D004151:Dipeptides / Q000378:metabolism; D004910:Erythrocyte Membrane / Q000378:metabolism / Q000502:physiology*; D004912:Erythrocytes / Q000276:immunology* / Q000378:metabolism / Q000502:physiology*; D006454:Hemoglobins / Q000378:metabolism; D006461:Hemolysis; D008970:Molecular Weight; D009211:Myoglobin / Q000378:metabolism; D009522:Newcastle disease virus / Q000276:immunology*; D012709:Serum Albumin / Q000378:metabolism; D012756:Sheep
D004151:Dipeptides; D006454:Hemoglobins; D009211:Myoglobin; D012709:Serum Albumin; D007611:Aprotinin