Molecular cloning of extensive sequences of the in vitro synthesized chicken ovalbumin structural gene.
Double-stranded DNA molecules complementary to ovalbumin chicken messenger RNA were synthesized in vitro and integrated into the E. coli plasmid pCR1 using an oligodG-dc tailing procedure. The resultant hybrid plasmids, amplified by transfection of E. coli, were shown by hybridization and gel electrophoresis to contain extensive DNA sequences of the ovalbumin structural gene.
D000818:Animals; D002645:Chickens; D002999:Clone Cells; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000378:metabolism; D003851:Deoxyribonucleases; D004926:Escherichia coli / Q000378:metabolism; D005260:Female; D005796:Genes; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D009838:Oligodeoxyribonucleotides / Q000032:analysis; D010047:Ovalbumin / Q000235:genetics*; D010957:Plasmids; D012333:RNA, Messenger / Q000378:metabolism
D004274:DNA, Recombinant; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D010047:Ovalbumin; D003851:Deoxyribonucleases; D004262:DNA Restriction Enzymes