title: Temporal changes in the hypothalamic and serum luteinizing hormone-releasing hormone (LH-RH) levels and the circulating ovarian steroids during the rat oestrous cycle.
abstract: Cycling female rats were sacrificed at various times during the 4-day oestrous cycle. LH-RH in the medial basal hypothalamus (MBH) and serum LH-RH, LH, oestradiol (Oe2), and progesterone were analyzed by radioimmunoassays. The MBH LH-RH content was lowest at 19.55-20.15 h during pro-oestrus but increased gradually  through oestrus and dioestrus I to significantly higher values at noon of dioestrus II, and then decreased precipitously at 18.00 h. Although serum LH levels remained basal from midnight of pro-oestrus through dioestrus II, serum LH-RH levels were significantly elevated at 12.00 and 21.00 h on oestrus (vs. the midnight pro-oestrus levels) and declined between 15.00 and 22.00 h of dioestrus I. In conjunction with high Oe2 titres, LH-RH in the MBH and serum declined initially during pro-oestrus between 14.07-14.45 h and then increased abruptly between 14.55-15.55 h; the pre-ovulatory rise in serum LH was observed from 16.05 h onwards. LH-RH activity in the MBH and serum receded gradually to the early afternoon levels by 19.55-24.00 h while the peak in serum LH was observed at 17.05-18.05 h. These studies suggested that the hypersecretion of the MBH LH-RH (synthesis + release) may be responsible for the pre-ovulatory discharge of LH.
mesh_terms: D000818:Animals; D004958:Estradiol / Q000097:blood*; D004971:Estrus; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000097:blood / Q000378:metabolism*; D007031:Hypothalamus / Q000378:metabolism; D007986:Luteinizing Hormone / Q000097:blood; D011247:Pregnancy; D011374:Progesterone / Q000097:blood*; D011863:Radioimmunoassay; D051381:Rats
chemical_list: D007987:Gonadotropin-Releasing Hormone; D011374:Progesterone; D004958:Estradiol; D007986:Luteinizing Hormone