[High-voltage electrophoresis of dihydrofolate reductase from Escherichia coli W 3110 (author's transl)].
A method for the high-voltage electrophoresis of dihydrofolate reductase from Escherichia coli W 3110 is described. Dihydrofolate reductase catalyses the reduction of dihydrofolic acid to tetrahydrofolic acid. By addition of a tetrazolium salt, tetrahydrofolic acid reacts by formation of a violet water insoluble formazane which is an indicator for the enzyme. Besides several unspecific bands, two isoenzymes of the dihydrofolate reductase from Escherichia coli W 3110 are found which are specificially inhibited by the folate antagonists methotrexate and trimethoprime in a concentration of 0, 1muM, 1 muM respectively.
D004588:Electrophoresis, Cellulose Acetate; D004926:Escherichia coli / Q000201:enzymology*; D005493:Folic Acid Antagonists; D007527:Isoenzymes / Q000032:analysis / Q000037:antagonists & inhibitors; D008727:Methotrexate / Q000494:pharmacology; D013762:Tetrahydrofolate Dehydrogenase / Q000032:analysis*; D014295:Trimethoprim / Q000494:pharmacology
D005493:Folic Acid Antagonists; D007527:Isoenzymes; D014295:Trimethoprim; D013762:Tetrahydrofolate Dehydrogenase; D008727:Methotrexate
Hochspannungselektrophorese der Dihydrofolatreduktase aus Escherichia coli W 3110