[Permeability of dextran-bound nuclease through the vascular barrier and tumor cell membrane].
Under study was the permeability of nuclease Ser marcescens, bound covalently by the method of diazocombinations with m-amino benzyloxymethyl-dextran of the molecular weight 20 000, 40 000, 60 000 via the vascular barrier and the membrane of tumor cells. It is shown that dextran-bound nuclease as well as the native enzyme would penetrate into the intacts cells of Ehrlich ascites carcinoma. The rate of the modified nuclease penetration is dependent on dextran molecular weight. Along with the native enzyme also nuclease bound to dextran of the molecular weight 20 000 and 40 000 penetrates the vascular barrier. The permeability of the vascular barrier for dextran-bound nuclease is found to be considerably higher in tumor-bearing animals than in intact ones.
D000818:Animals; D001667:Binding, Competitive; D002199:Capillary Permeability; D002286:Carcinoma, Ehrlich Tumor / Q000473:pathology*; D002463:Cell Membrane Permeability; D003851:Deoxyribonucleases / Q000378:metabolism*; D003911:Dextrans / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D012706:Serratia marcescens / Q000201:enzymology
D003911:Dextrans; D003851:Deoxyribonucleases
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