title: Studies on mutagenicity of irradiated sugar solutions in Salmonella typhimurium.
abstract: Irradiated sugar solutions are mutagenic towards Salmonella typhimurium, the effect being dose-dependent up to 2.0 Mrad. At all doses, ribose solution exhibited greater mutagenicity than did sucrose solution. The mutagenic effect was observed only in dividing cells and appears to be directly related to the growth rate. A larger proportion of revertants was observed after incubation with irradiated sugar solution for a period of 4 h than for 24 h. Irradiation of the sugar solutions in the frozen conditions was effective in completely preventing the development of mutagenic potential. Post-irradiation storage of the sugar solutions for a prolonged period (25 weeks) also minimized their mutagenic effect. The irradiated sugar solutions gave rise to both missense and frame-shift (additon as well as deletion) types of mutation; ribose was more effective in inducing the latter type. The irradiated sugar solutions failed to show a mutagenic response in the host-mediated assay with mice as the mammalian host.
mesh_terms: D000693:Anaerobiosis; D000818:Animals; D002455:Cell Division; D005720:Gamma Rays; D051379:Mice / Q000382:microbiology; D009153:Mutagens; D011309:Preservation, Biological; D012266:Ribose / Q000494:pharmacology* / Q000528:radiation effects; D012486:Salmonella typhimurium / Q000187:drug effects*; D013395:Sucrose / Q000494:pharmacology / Q000528:radiation effects*
chemical_list: D009153:Mutagens; D013395:Sucrose; D012266:Ribose