Functional, structural, and antigenic similarities of guinea pig anti-phosphorylcholine antibodies.
The humoral immune response to PC was measured in guinea pigs. PC-vaccine stimulated IgM and IgG2, but little IgG1, anti-PC -antibodies. No memory was induced and immunization in CFA produced tolerance. PC-KLH, on the other hand, stimulated IgM, IgG2, and IgG1 anti-PC antibodies with carrier-specific memory. Hapten inhibition of plaque formation showed uniform binding patterns with minor, but significant, differences between antiPC-vaccine and anti-PC-KLH antibodies. The antibodies were characterized by IEF and idiotypic analyses. Early after immunization with PC-vaccine, guinea pigs had restricted IEF patterns which in inbred, but not outbred animals were indistinguishable between individuals. These patterns remained restricted but more individualized with time after immunization. Anti-PC-KLH antibodies showed more heterogeneity and individuality. However, these structurally heterogeneous antibodies reacted equivalently with rabbit anti-idiotype antisera and therefore must share common structural features, regardless of isotype or the genetic background of the guinea pig.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D000917:Antibody Formation; D000918:Antibody Specificity; D002794:Choline / Q000031:analogs & derivatives*; D005838:Genotype; D006168:Guinea Pigs; D006433:Hemocyanins / Q000276:immunology; D006462:Hemolytic Plaque Technique; D007106:Immune Sera / Q000494:pharmacology; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D007525:Isoelectric Focusing; D008297:Male; D010767:Phosphorylcholine / Q000276:immunology*; D014612:Vaccines / Q000494:pharmacology
D000906:Antibodies; D007106:Immune Sera; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D014612:Vaccines; D010767:Phosphorylcholine; D006433:Hemocyanins; D002794:Choline