Primary in vitro immunogenicity of liposomal model membranes in mouse spleen cell cultures.
Neither 2,4-dinitrophenyl-6-N-aminocaproylphosphatidylethanolamine (DNP-Cap-PE) nor fluoresceinthiocarbamylphosphatidylethanolamine (F1-PE) induces hapten-specific plaque-forming cells (PFC) when incubated with suspensions of spleen cells from unimmunized C57BL/6J mice. However, PFC are produced after incorporation of these synthetic lipid antigens into liposomal model membranes. The in vitro response is characterized by the following: a) it is time and dose dependent; b) the frequency of IgM PFC exceeds IgG PFC; c) both nonadherent and adherent cells are required (2-mercaptoethanol can replace the requirement for adherent cells in some experiments); d) depletion of thymus-derived cells by treatment with anti-theta antiserum plus complement does not diminish the response; e) spleen cells from nude BALB/c mice also produce PFC. Thus, the essential features of the in vivo immunogenicity of DNP-Cap-PE and F1-PE sensitized liposomes, which have been previously described, can be replicated in an in vitro cell culture system.
D000614:Aminocaproates; D015119:Aminocaproic Acid / Q000276:immunology; D000818:Animals; D002462:Cell Membrane / Q000276:immunology; D002478:Cells, Cultured; D004136:Dinitrobenzenes / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D005452:Fluoresceins / Q000276:immunology; D007700:Kinetics; D008081:Liposomes / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D008954:Models, Biological; D010714:Phosphatidylethanolamines / Q000276:immunology; D013154:Spleen / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
C021015:2,4-dinitrophenyl-6-N-aminocaproylphosphatidylethanolamine; D000614:Aminocaproates; D004136:Dinitrobenzenes; D005452:Fluoresceins; D008081:Liposomes; D010714:Phosphatidylethanolamines; C021026:fluoresceinthiocarbamylphosphatidylethanolamine; D015119:Aminocaproic Acid