title: Sera from volunteers immunized by planned blood transfusions as source of DR cytotoxic typing reagents.
abstract: Sera from volunteers immunized with planned blood transfusions were tested for anti DR cytotoxic antibodies with a panel of HLA typed cultured human lymphoid cells using a variety of serological techniques. The majority of sera contained DR cytotoxic antibodies. The specificity of DR antibodies in seven sera was determined by testing them with a panel of B peripheral lymphocytes typed with DR alloantisera submitted to the 7th International Histocompatibility Workshop. The temporal evolution of DR and HLA-A and B cytotoxic antibodies was determined in two subjects by testing serial bleedings with B lymphoid cells, coated with Fab2 fragments from anti beta 2 mu and anti DR xenoantisera. Results indicated a parallel evolution of DR and HLA-A and B cytotoxic antibodies.
mesh_terms: D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000276:immunology; D001803:Blood Transfusion; D002478:Cells, Cultured; D003601:Cytotoxicity Tests, Immunologic; D006680:HLA Antigens; D006801:Humans; D007106:Immune Sera; D007114:Immunization; D007202:Indicators and Reagents; D008214:Lymphocytes / Q000276:immunology
chemical_list: D006680:HLA Antigens; D007106:Immune Sera; D007202:Indicators and Reagents