title: Hexokinase of Angiostrongylus cantonensis: presence of a glucokinase.
abstract: 1. Angiostrongylus cantonensis contains a glucokinase which was isolated by DEAE-cellulose column chromatography. 2. This enzyme has a much higher affinity toward glucose (apparent Km, 0.2 mM) than fructose (apparent Km, 85 mM). Glucose-6-phosphate (10 mM) does not inhibit glucose phosphorylation. 3. Molecular weight obtained by a molecular sieve chromatography (60,000) is also close to the value of mammalian glucokinase. 4. While Vmax value for mannose is one-third smaller than that for glucose, Km for mannose is rather lower than that for glucose. 5. In addition to the cytosol enzyme, a particle bound hexokinase is found in the worm.
mesh_terms: D000801:Angiostrongylus / Q000201:enzymology*; D000818:Animals; D005260:Female; D005941:Glucokinase / Q000302:isolation & purification* / Q000378:metabolism; D006593:Hexokinase / Q000302:isolation & purification* / Q000378:metabolism; D007700:Kinetics; D008297:Male; D008681:Metastrongyloidea / Q000201:enzymology*
chemical_list: D006593:Hexokinase; D005941:Glucokinase