Mapping of a gene for a major outer membrane protein of Escherichia coli K12 with the aid of a newly isolated bacteriophage.
A method is described for the enrichment of phages which can adsorb to a specific determinant of bacterial cell surfaces. A phage was isolated which absorbs to E. coli cells containing the "major outer membrane= protein c but not to strains that are lacking this protein. With the aid of this phage a gene, meoA which is responsible for the lack of protein c was mapped at 48 min on the linkage map of E. coli K12.
D001426:Bacterial Proteins / Q000096:biosynthesis*; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D003090:Coliphages / Q000302:isolation & purification / Q000502:physiology*; D004926:Escherichia coli / Q000378:metabolism* / Q000648:ultrastructure; D005796:Genes; D008565:Membrane Proteins / Q000096:biosynthesis*; D014161:Transduction, Genetic; D010948:Viral Plaque Assay
D001426:Bacterial Proteins; D008565:Membrane Proteins