The laboratory diagnosis of Nairobi sheep disease.
The laboratory methods available for the isolation and identification of Nairobi sheep disease virus have been compared. The results show that inoculation of tissue culture (BHK 21 C 13) with suspensions of infected organs or plasma followed by fluorescent antibody tests on coverslip preparations gave the quickest means of identification. This test did not depend on the production of a cytopathic effect. Primary isolation of the virus in infant mouse brain and identification either by fluorescent antibody methods or by complement fixation with antigen prepared from the mouse brain offers a slightly more sensitive isolation system and would be recommended where no tissue culture facility exists.
D000818:Animals; D001103:Arboviruses / Q000254:growth & development / Q000276:immunology / Q000302:isolation & purification; D002478:Cells, Cultured; D003168:Complement Fixation Tests; D003588:Cytopathogenic Effect, Viral; D005455:Fluorescent Antibody Technique; D051379:Mice; D009265:Nairobi Sheep Disease / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D012756:Sheep