Sequence of the 3'-noncoding and adjacent coding regions of human gamma-globin mRNA.
In cloning human fetal globin cDNA in bacterial plasmids, we obtained a recombinant which contained a fragment of gammg-globin cDNA corresponding to the region from amino acid 99 to the poly A. We determined a sequence of 169 nucleotides which included the complete 3' non-coding region of the gamma-globin mRNA. The codon for amino acid 136 was GCA, indicating that this cloned fragment was derived from the Agamma-globin gene. In conjunction with the surrounding sequences, the GCA codon provides the Agamma-species with a unique CTGCAG hexanucleotide that is recognized by the restriction enzyme Pst I. The 3'-untranslated region of the gamma-globin mRNA consists of 90 nucleotides, and shares little homology with that of the human beta-globin mRNA. As in other mammalian mRNAs, a symmetrical sequence and the hexanucleotide AAUAAA are present.
D001483:Base Sequence; D003062:Codon; D004274:DNA, Recombinant / Q000378:metabolism; D005260:Female; D005333:Fetus; D005914:Globins / Q000096:biosynthesis*; D006801:Humans; D009838:Oligodeoxyribonucleotides / Q000032:analysis; D010957:Plasmids; D011247:Pregnancy; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis*; D014158:Transcription, Genetic
D003062:Codon; D004274:DNA, Recombinant; D009838:Oligodeoxyribonucleotides; D012333:RNA, Messenger; D005914:Globins