Subcellular distribution of newly synthesized glycerolipids in intact and homogenized rat adipocytes.
1. Homogenization of rat adipocytes was found to inhibit the transfer of newly synthesized phospholipids and glycerides from the membranes to the storage lipids. 2. In intact adipocytes, only 23 +/- 3.4% of the phospholipids and 0.44 +/- 0.12% of the glycerides remained bound to the membranes, while for the homogenate, the comparable values obtained were 74 +/- 5.2% and 90 +/- 0.3% respectively. 3. Various factors that might be involved in the liberation of the glycerolipids from the adipocyte esterification sites are discussed.
D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D005989:Glycerides / Q000096:biosynthesis*; D066298:In Vitro Techniques; D008297:Male; D008563:Membrane Lipids / Q000096:biosynthesis; D010743:Phospholipids / Q000096:biosynthesis*; D051381:Rats; D011919:Rats, Inbred Strains; D013347:Subcellular Fractions / Q000378:metabolism
D005989:Glycerides; D008563:Membrane Lipids; D010743:Phospholipids