Characterization of alpha-1-antitrypsin by isoelectric focusing on an ultrathin polyacrylamide gel layer. An economic high-resolution system for determining PiM subtypes.
Through the use of ultrathin layer polyacrylamide gel isoelectric focusing it is possible to obtain a resolution of the bands of alpha 1AT so as to be able to easily recognize all six PiM subtypes. The optimal resolution of the PiM subtypes is obtained without deforming the pattern of the Pi phenotypes. In addition to high resolution, ultrathin layer polyacrylamide gel isoelectric focusing permits a notable reduction of fees to 1/5 of the usual.
D000328:Adult; D000665:Ampholyte Mixtures; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D007525:Isoelectric Focusing / Q000379:methods*; D008297:Male; D010641:Phenotype; D000515:alpha 1-Antitrypsin / Q000032:analysis / Q000235:genetics*
D000665:Ampholyte Mixtures; D000515:alpha 1-Antitrypsin