Packaging recombinant DNA molecules into bacteriophage particles in vitro.
Recombinant phage genomes made in reactions with purified enzymes may be recovered directly by packaging into phage heads in vitro. The process is efficient and nonselective and offers containment in initial stages of handling recombinant DNA. Ligase [poly(deoxyribonucleotide):poly-(deoxyribonucleotide) ligase (AMP-forming), EC 6.5.1.1] reaction products can recombine with endogenous phage DNA during packaging, but UV-irradiation eliminates the biological activity of the endogenous DNA.
D003090:Coliphages / Q000378:metabolism* / Q000528:radiation effects; D011088:DNA Ligases / Q000378:metabolism; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000378:metabolism; D004274:DNA, Recombinant / Q000378:metabolism* / Q000528:radiation effects; D004279:DNA, Viral / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism / Q000528:radiation effects; D005838:Genotype; D009154:Mutation; D013045:Species Specificity; D014162:Transfection; D014466:Ultraviolet Rays
D004269:DNA, Bacterial; D004274:DNA, Recombinant; D004279:DNA, Viral; D004262:DNA Restriction Enzymes; D011088:DNA Ligases