Role of viral infectivity in the induction of influenza virus-specific cytotoxic T cells.
This report examines the requirement for infectious virus in the induction of influenza virus-specific cytotoxic T cells. Infectious influenza virus was found to be highly efficient at generating both primary and secondary cytotoxic T-cell response in vivo. Inactivated influenza virus however, failed to stimulate a detectable cytotoxic T-cell response in vivo even at immunizing doses 10(5)-10(6)-fold higher than the minimum stimulatory dose of infectious virus. Likewise inactivated virus failed to sensitize target cells for T cell-mediated lysis in vitro but could stimulate a specific cytotoxic response from primed cells in vitro. Possible requirements for the induction of virus-specific cytotoxic T-cell responses are discussed in light of these observations and those of other investigators.
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D000917:Antibody Formation; D003602:Cytotoxicity, Immunologic; D006389:Hemagglutinins, Viral; D007111:Immunity, Cellular; D007116:Immunization, Passive; D007156:Immunologic Memory; D009980:Influenza A virus / Q000276:immunology* / Q000528:radiation effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes / Q000276:immunology*; D014466:Ultraviolet Rays
D000914:Antibodies, Viral; D006389:Hemagglutinins, Viral