The dissociation of sigma-factor from ribonucleic acid polymerase.
The sigma-factor of Escherichia coli RNA polymerase was shown to dissociate from the core enzyme as a function of absolute concentration. The association constant is in the range 10(6)-10(8) litre/mol. This implies that the amount of holoenzyme, core enzyme and sigma-factor in RNA polymerase assays may vary according to the absolute concentration of the enzyme.
D012321:DNA-Directed RNA Polymerases; D004926:Escherichia coli / Q000201:enzymology*; D007834:Lasers; D008970:Molecular Weight; D009994:Osmolar Concentration; D012542:Scattering, Radiation; D012808:Sigma Factor; D014157:Transcription Factors
D012808:Sigma Factor; D014157:Transcription Factors; D012321:DNA-Directed RNA Polymerases