Interaction of DNA with DNA binding proteins. II. Displacement of Escherichia coli DNA unwinding protein and the condensed structure of DNA complexed with protein HD.
Three DNA binding proteins from Escherichia coli cells have been complexed with single-stranded phage fd DNA. Electron microscopy reveals granular substructures in the complexes formed with protein HD. In complexes of DNA unwinding protein with fd DNA both protein HD and phage-coded gene 5 protein partially displace the unwinding protein which results in the formation of structures characteristic for the DNA complexes formed with either protein HD or gene 5 protein alone. Combination of protein HD with double-stranded phage T7 DNA leads to a progressive folding and condensing of the genome. The structures observed are discussed in relation to current concepts of the packing of DNA in protein complexes.
D001426:Bacterial Proteins; D002352:Carrier Proteins; D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D004926:Escherichia coli; D008854:Microscopy, Electron; D014764:Viral Proteins
D001426:Bacterial Proteins; D002352:Carrier Proteins; D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D014764:Viral Proteins