[T2 DNA, modified by 2,2,6,6-tetramethyl-4-bromoacetooxypiperidine-i-oxyl as a template for RNA polymerase from E. coli B].
T2-DNA was modified by 2,2,6,6-tetramethyl-4-bromoacetooxypiperidine-1-oxyl (I) at different NaCl concentrations (10(-1) M NaCl--10(-4) M NaCl). Modified DNA were investigated as templates for the RNA-polymerase from E. coli B. It was shown that T2-DNA modified I in 0,1 M NaCl completely preserves the native secondary structure, has a low degree modification (1 molecule I per 1000-2000 nucleotide pairs), but is a noneffective template for the RNA-polymerase from E. coli B (20%-40% as compared with unmodified T2-DNA). Under these conditions the modification occurs probably at the "weakest" (readily melting) sites of DNA. The role of these "weak" sites on DNA as promotors is discussed. The modification of T2-DNA by reagnet I has a stronger inhibitory effect on the total RNA synthesis than on the RNA-synthesis stable to rifampicin. Possible existence of two kinds of "early" promotors on T2-DNA is assumed.
D003090:Coliphages; D003497:Cyclic N-Oxides; D004279:DNA, Viral / Q000378:metabolism* / Q000494:pharmacology; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology*; D010880:Piperidines; D012313:RNA / Q000096:biosynthesis; D012293:Rifampin / Q000494:pharmacology; D013329:Structure-Activity Relationship; D013698:Templates, Genetic
D003497:Cyclic N-Oxides; D004279:DNA, Viral; D010880:Piperidines; D012313:RNA; D012321:DNA-Directed RNA Polymerases; D012293:Rifampin
Izuchenie matrichnykh svoĭstv T2-DNK, modifitsirovannoĭ 2,2,6,6,-tetrametil-4-bromatsetoksipiperidin-1--oksilom, v RNK-polimeraznoĭ sisteme E. coli B.