Five hundredfold overproduction of DNA ligase after induction of a hybrid lambda lysogen constructed in vitro.
A lambda vector that contains the gene for Escherichia coli DNA ligase (lambdagt4-lop-11 lig+) has been modified to achieve overproduction of this enzyme. The third Eco RI site in the lambda chromosome has been altered by mutation, and the left-hand Eco RI fragment has been shortened. The new vector, lambdagt4-lop-11 lig+, forms a stable lysogen which, upon induction, produces a 100-fold increase in DNA ligase activity. Introduction of a phage mutation (S7) that prevents cell lysis results in an even greater increase (500-fold).
D003090:Coliphages / Q000378:metabolism*; D004269:DNA, Bacterial / Q000378:metabolism; D004274:DNA, Recombinant / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism*; D004926:Escherichia coli; D005796:Genes; D008242:Lysogeny; D009154:Mutation; D011118:Polynucleotide Ligases / Q000096:biosynthesis*
D004269:DNA, Bacterial; D004274:DNA, Recombinant; D004279:DNA, Viral; D011118:Polynucleotide Ligases