[The mechanization of the enzyme immunological determination of phenytoine with the Centrifichem (author's transl)].
The separation of free from bound antigen is not required in homogeneous enzyme immunoassays (EMIT). Therefore this technique can easily be mechanized. For the mechanization of the enzyme immunological determination of serum levels of Phenytoine the fast analyzer (CENTRIFICHEM) was found most satisfactory in our laboratory. The marked advantages over manual methods are: 1. The amount of sample and reagents needed can be reduced by half. 2. The intra- and interassay variance are significantly better compared to values found with manual techniques. 3. 60 samples can be determined in duplicate in under two hours.
D001322:Autoanalysis / Q000379:methods; D002498:Centrifugation / Q000379:methods; D006801:Humans; D007124:Immunoenzyme Techniques; D010672:Phenytoin / Q000097:blood*
D010672:Phenytoin
Zur Mechanisierung der enzymimmunologischen Bestimmung von Phenytoin mit dem Centrifichem