The association of serum proteins with peripheral blood cells at low ionic strength: ultrastructural examination.
Peripheral white blood cells have been shown to bind serum immunoglobins when prepared in low ionic strength sucrose solutions. The presence and distribution of Ig on neutrophils, monocytes and lymphocytes was described using an immunoferritin probe and the electron microscope. It appears that this approach reveals immunoglobulins associated with peripheral white blood cells that are not observed by the usual methods of examining these cell surfaces after preparation at physiological ionic strength.
D000888:Antibodies, Anti-Idiotypic; D005293:Ferritins; D006801:Humans; D008214:Lymphocytes / Q000032:analysis*; D008854:Microscopy, Electron; D009000:Monocytes / Q000032:analysis*; D009504:Neutrophils / Q000032:analysis*; D009994:Osmolar Concentration; D011947:Receptors, Antigen, B-Cell / Q000032:analysis*
D000888:Antibodies, Anti-Idiotypic; D011947:Receptors, Antigen, B-Cell; D005293:Ferritins