title: Peritoneal exudate cells. V. Influence of age, sex, strain and species on the induction and the growth of macrophage colony forming cells.
abstract: We investigated the effects of age, sex and strain in the induction of peritoneal exudate colony-forming cells (PE-CFC) in mice. Sex and age (ranging from 3 weeks to 12 months) had no significant effect on the induction of PE-CFC. However, we found significant difference between strains in terms of the number of nucleated cells and the proportion of PE-CFC in exudate cells. When we investigated the mechanisms behind this strain difference, we found that it was due to neither the type of colony-stimulating factor employed in culture, nor the type of stimulants used to induce the exudate, nor the difference in the kinetics of appearance of PE-CFC in the peritoneal cavity. We also studied the induction of PE-CFC in rats and hamsters and the growth of these cells in vitro. Unlike mouse cells, PE-CFC from rats and hamsters could also use media conditioned by cells from other species as a source of colony-stimulating factor.
mesh_terms: D000367:Age Factors; D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology*; D001854:Bone Marrow Cells; D002452:Cell Count; D002999:Clone Cells / Q000378:metabolism; D003115:Colony-Stimulating Factors / Q000378:metabolism*; D006224:Cricetinae; D006023:Glycoproteins / Q000378:metabolism*; D007700:Kinetics; D008264:Macrophages / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D008815:Mice, Inbred Strains; D051381:Rats; D012737:Sex Factors; D013045:Species Specificity
chemical_list: D003115:Colony-Stimulating Factors; D006023:Glycoproteins