Quantitation of basophil-bound IgE in atopic and nonatopic subjects.
Basophil-bound IgE was measured by quantitative immunofluorescence microscopy in atopic subjects and in healthy controls. A correlation was found between IgE serum level and basophil-bound IgE. The basophils from the atopic patients, both from those with a low and those with an increased serum IgE, showed a significantly higher fluorescence intensity than the basophils from the respective controls. Our results indicate that both atopy and the IgE level of the serum determine the amount of basophil-bound IgE.
D000293:Adolescent; D000328:Adult; D001491:Basophils / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000276:immunology*; D007073:Immunoglobulin E / Q000032:analysis*; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged
D007073:Immunoglobulin E