Dynamics of x-ray-induced reversion in heterogeneous S. cerevisiae populations.
The survival and frequency of adenine and homoserine revertants after X-irradiation have been studied in starved and growing populations of haploid S. cerevisiae (strain 5483/1b). A growing population is heterogeneous to cell killing, and a mathematical model can be used to determine possible correlation between sensitivity to killing and sensitivity to mutation induction. The results indicate correlation between sensitivity to ade2-1 reversion and sensitivity to cell killing, whereas no such correlation was found between sensitivity to hom3-10 reversion and sensitivity to killing. The difference in the dynamics of homoserine and adenine reversions was reduced by adding caffeine to the post-irradiation media.
D000225:Adenine / Q000378:metabolism; D002110:Caffeine / Q000494:pharmacology; D004307:Dose-Response Relationship, Radiation; D006714:Homoserine / Q000378:metabolism; D009154:Mutation / Q000187:drug effects / Q000528:radiation effects*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D014965:X-Rays
D002110:Caffeine; D006714:Homoserine; D000225:Adenine