Metabolism of the polysaccharides of human dental plaque. Part II. Purification and properties of Cladosporium resinae (1 leads to 3)-alpha-D-glucanase, and the enzymic hydrolysis of glucans synthesised by extracellular D-glucosyltransferases of oral streptococci.
Cladosporium resinae (1 leads to 3)-alpha-D-glucanase has been characterized as an endoglucanase capable of completely hydrolysing insoluble (1 leads to 3)-alpha-D-glucans isolated from fungal cell-walls. D-Glucose was the major product, but a small amount of nigerose was also produced. The enzyme was specific for the hydrolysis of (1 leads to 3) bonds that occur in sequence, and nigerotetraose was the smallest substrate that was rapidly attacked. Isolated (1 leads to 3)-alpha-D-glucosidic linkages that occur in mycodextran, isolichein, dextrans, and oligosaccharides derived from dextran were not hydrolysed. Insoluble glucan synthesised from sucrose by culture filtrates of Streptococcus  spp. were all hydrolysed to various limits; the range was 11-61%. A soluble glucan, synthesised by an extracellular D-glucosyltransferase of S. mutans OMZ176, was not a substrate, whereas insoluble glucans synthesised by a different D-glucosyltransferase, isolated from S. mutans strains OMZ176 and K1-R, were extensively hydrolysed (84 and 92%, respectively). It is suggested that dextranase-CB, a bacterial endo(1 leads to 6)-alpha-D-glucanase that does not release D-glucose from any substrate, could be used together with C. resinae (1 leads to 3)-alpha-D-glucanase to determine the relative proportions of (1 leads to 6)-linked to (1 leads to 3)-linked sequences of D-glucose residues in the insoluble glucans produce by oral streptococci. The simultaneous action of the two D-glucanoses was highly effective in solubilizing the glucans.
D002963:Cladosporium / Q000201:enzymology*; D003773:Dental Plaque; D005947:Glucose; D005959:Glucosidases / Q000378:metabolism*; D005964:Glucosyltransferases / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D003904:Mitosporic Fungi / Q000201:enzymology*; D011134:Polysaccharides; D011135:Polysaccharides, Bacterial; D013045:Species Specificity; D013291:Streptococcus; D013379:Substrate Specificity; D000520:alpha-Glucosidases / Q000302:isolation & purification / Q000378:metabolism*
D011134:Polysaccharides; D011135:Polysaccharides, Bacterial; D005964:Glucosyltransferases; D005959:Glucosidases; D000520:alpha-Glucosidases; D005947:Glucose