Lactate dehydrogenase isozymes in the spermatozoa of the domestic fowl, Gallus domesticus and turkey, Meleagris gallopavo.
1. Electrophoresis of extracts of turkey spermatozoa for lactate dehydrogenase activity revealed the usual five tissue LDHs (LDH-1 to LDH-5). 2. The presence of LDH-X (the spermatozoan-specific isozyme) was not obvious. 3. Only one band was present on electrophoresis of fowl spermatozoan extracts and it coincided with LDH-1 (heart type). 4. Kinetic investigations, the use of inhibitors and the heat-stability test confirmed that the fowl spermatozoan LDH was probably LDH-1 and not LDH-X.
D000818:Animals; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000032:analysis*; D008297:Male; D011200:Poultry / Q000378:metabolism*; D013094:Spermatozoa / Q000201:enzymology*; D014422:Turkeys / Q000378:metabolism*
D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase