title: Structural examination of antitumour, water-soluble glucans from Grifora umbellata by use of four types of glucanase.
abstract: Antitumour glucans (GU) from the fungus Grifora umbellata have been subjected to periodate oxidation, Smith degradation, methylation analysis, and treatment with endo-(1 leads to 6)-beta-D-, endo-(1 leads to 3)-beta-D-, and exo-(1 leads to 3)-beta-D-glucanases, and alpha-amylase; the following structural features were revealed. GU-2 contains a backbone involving (1 leads to 6)-beta- and () leads to 3)-beta linkages, and two kinds of branches involving (1 leads to 6)-beta and (1 leads to 4)-alpha linkages. GU-3 has a (1 leads to 3)-beta-linked backbone and branches involving (1 leads to 6)-beta linkages or (1 leads to 4)-alpha and (1 leads to 6)-beta linkages. GU-4 also contains a (1 leads to 3) beta-D-glucan backbone and a small number of (1 leads to 6)-beta-linked branches. Probable structural units of these glucans are proposed.
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use; D055598:Chemical Phenomena; D002621:Chemistry; D005260:Female; D005658:Fungi / Q000032:analysis*; D005936:Glucans / Q000627:therapeutic use; D006026:Glycoside Hydrolases; D051379:Mice; D012510:Sarcoma 180 / Q000188:drug therapy; D000516:alpha-Amylases
chemical_list: D000970:Antineoplastic Agents; D005936:Glucans; D006026:Glycoside Hydrolases; D000516:alpha-Amylases