A simple multiple chamber apparatus for measuring chemotaxis of polymorphonuclear leukocytes utilizing centrifugation of the chambers before incubation.
A simple apparatus for measuring chemotaxis is described which contains thirty chambers per unit. Only very few cells and small volumes of chemotactic factor solution are required per chamber. For satisfactory results the apparatus must be centrifuged briefly prior to incubation. Under these conditions results can be obtained that are equivalent to or, with short incubation times, superior to other commonly used modified Boyden chambers.
D002498:Centrifugation; D002634:Chemotaxis, Leukocyte; D004306:Dose-Response Relationship, Immunologic; D007158:Immunologic Techniques / Q000295:instrumentation; D009504:Neutrophils; D013997:Time Factors