Composition of human granulocytic colonies as a function of the purification stage of CSF.
Granulopoietic colonies grown in presence of human placental conditioned medium (HPCM), before and after partial purification, were characterized in terms of number of colonies, colony size and cellular composition. Our data demonstrate that the removal of inhibitors present in crude HPCM preparations results in a higher number of CFUC which are wider in size and with different cellular composition.
D001854:Bone Marrow Cells; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D003115:Colony-Stimulating Factors / Q000032:analysis / Q000302:isolation & purification*; D003470:Culture Media; D005260:Female; D006098:Granulocytes / Q000166:cytology*; D006410:Hematopoiesis; D006801:Humans; D007962:Leukocytes / Q000166:cytology; D010920:Placenta; D011247:Pregnancy
D003115:Colony-Stimulating Factors; D003470:Culture Media