The cellular mechanism of leukocyte adherence inhibition.
Human blood leukocytes from three subjects who had been contact sensitized to dinitrochlorobenzene were used in direct and indirect leukocyte-adherence-inhibition (LAI) reactions in an attempt to elucidate the cellular mechanism of reactivity. The leukocytes were separated and purified by standard procedures. In direct LAI, only T cells or populations containing T cells gave positive reactions (significantly reduced adherence) with the antigen. Supernatants from suitable leukocyte-antigen mixtures contained a soluble leukocyte-adherence-inhibition-factor (LAIF) that reduced the adherence of normal leukocytes. Only T cells or populations containing T cells were active in LAIF production; B cells, granulocytes, and monocytes were inactive. The cellular requirement for the action of preformed LAIF was not restricted: all major types of blood leukocytes were susceptible to its effect.
D001402:B-Lymphocytes / Q000276:immunology; D001557:Benzenesulfonates / Q000276:immunology; D002448:Cell Adhesion; D004136:Dinitrobenzenes / Q000276:immunology; D004137:Dinitrochlorobenzene / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D006098:Granulocytes / Q000276:immunology; D006801:Humans; D007111:Immunity, Cellular; D007700:Kinetics; D007957:Leukocyte Adherence Inhibition Test; D009000:Monocytes / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D001557:Benzenesulfonates; D004136:Dinitrobenzenes; D004137:Dinitrochlorobenzene