title: Lambdoid phages that simplify the recovery of in vitro recombinants.
abstract: Derivatives of phage lambda are described for use as vectors for fragments of DNA generated with the HindIII and EcoRI restriction enzymes. With some vectors, hybrid molecules are recognised by a change from a turbid to a clear plaque morphology resulting from the insertion of a fragment of DNA into the lambda gene coding for the phage regressor. Other vectors contain a central, replaceable fragment of DNA which imparts a readily recognisable phenotype. This central fragment may include either a gene for a mutant transfer RNA (suppressor) or a part of the lacZ gene of E. coli able to complement a lacZ host. The appropriate lacZ host and indicator plates permit the ready distinction between recombinant and vector phages by the colour of the plaques.
mesh_terms: D003090:Coliphages; D004274:DNA, Recombinant; D004279:DNA, Viral; D004926:Escherichia coli; D005796:Genes; D005816:Genetic Complementation Test; D011995:Recombination, Genetic; D010948:Viral Plaque Assay
chemical_list: D004274:DNA, Recombinant; D004279:DNA, Viral