[Production of lymphotoxin by human lymphocytes. II. Stimulation by purified tuberculin].
A total of 43 lymphocyte culture (LC) from 12 adults (aged 32--48 years) and 21 children 6--7 years of age were studied. In PPD-stimulated adults LC stimulation ratio ranged from 8 to 39%; their cytotoxic activity, i.e. lymphotoxin (LT) production, was determined by staining target L cells with crystal violet and measuring protein synthesis in surviving target cells by radioactive amino acid incorporation. In children blastogenic response occurred in 11 out of 21 PPD-stimulated LC studied, but only 8 supernatant culture fluids were toxic to L cells and 3 were not. These findings were confirmed by repeated tests 3 weeks later. Probable correlation of BTL and LT production with the functional state of specific immunity to tuberculosis is discussed.
D000328:Adult; D002478:Cells, Cultured; D002648:Child; D006801:Humans; D007739:L Cells; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism*; D008233:Lymphotoxin-alpha / Q000032:analysis / Q000096:biosynthesis*; D008875:Middle Aged; D010835:Phytohemagglutinins / Q000494:pharmacology; D014373:Tuberculin / Q000276:immunology*; D014376:Tuberculosis / Q000276:immunology*
D008233:Lymphotoxin-alpha; D010835:Phytohemagglutinins; D014373:Tuberculin
Produktsiia limfotoksina limfotsitami cheloveka. Soobshchenie II. Stimuliatsiia ochishchennym tuberkulinom.