Haemolytic assays in agarose plates for components of the classical complement pathway: interference by the alternative pathway.
It has been observed that when serum C6 is measured by the haemolytic radial diffusion technique a heat labile factor limits the size of the haemolytic rings. This reduction is haemolysis has been shown to be due to alternative pathway activation of C6 in the agarose plate; and that the heat labile factor is Factor B of the alternative pathway. This phenomenon is of practical importance when assaying for C6; however, it does not explain the observations of a C6 inactivator reported by Nelson & Biro (1968).
D000936:Antigen-Antibody Complex; D003182:Complement C5 / Q000037:antagonists & inhibitors; D003183:Complement C6 / Q000032:analysis* / Q000037:antagonists & inhibitors; D003184:Complement C7 / Q000037:antagonists & inhibitors; D003165:Complement System Proteins / Q000032:analysis* / Q000378:metabolism; D006462:Hemolytic Plaque Technique; D006358:Hot Temperature; D006801:Humans; D012685:Sepharose
D000936:Antigen-Antibody Complex; D003182:Complement C5; D003183:Complement C6; D003184:Complement C7; D003165:Complement System Proteins; D012685:Sepharose