Development of delayed-type hypersensitivity during Mycobacterium lepraemurium infection in mice.
Various preparations of Mycobacterium lepraemurium were used to elicit delayed-type hypersensitivity in the footpad of mice infected with this organism. With a sonicated preparation of the mycobacterium, a significant increase in footpad swelling was elicited in mice infected with M. lepraemurium 5 weeks previously, but not in BCG-infected animals or uninfected controls. This footpad reaction was shown to peak at 24 h and to be associated with an infiltration of mononuclear cells. The kinetics of footpad swelling, its association with lymphoproliferation, and its dependence on T lymphocytes were each examined. The results support the hypothesis that this is a delayed-type hypersensitivity reaction. The ability to transfer this reactivity to normal mice with cells but not serum offers further confirmation that this hypersensitivity is dependent on cell-mediated immunological mechanisms rather than humoral antibody. The relevance of this to the study of the immunological response of mice to murine leprosy is discussed.
D000818:Animals; D000942:Antigens, Bacterial; D001202:Ascitic Fluid / Q000166:cytology; D001500:BCG Vaccine; D006968:Hypersensitivity, Delayed / Q000276:immunology* / Q000473:pathology; D007106:Immune Sera; D007116:Immunization, Passive; D008198:Lymph Nodes / Q000473:pathology; D051379:Mice; D009164:Mycobacterium Infections / Q000276:immunology* / Q000473:pathology; D009163:Mycobacterium bovis / Q000276:immunology; D009167:Mycobacterium lepraemurium / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
D000942:Antigens, Bacterial; D001500:BCG Vaccine; D007106:Immune Sera