Spectrophotometry as a tool for assaying endotoxins.
A spectrophotometric method is reported for assaying endotoxins over a wide concentration range, including low parts per billion levels. Five solution of endotoxins. Escherichia coli 0127:B8, Escherichia coli 055:B5, Salmonella abortus-equi, Salmonella enteritidis, and Shigella flexneri, were examined. Each exhibited an absorption maximum at 259 nm, but the absorptivities differed in each case. Thus, rigorous quantification requires that the identity of the endotoxin be known. The uv absorbance and the statistical data for the standard curves for three groups of standard solutions are presented. The absorbance for each group was linear with concentration within acceptable limits.
D004731:Endotoxins / Q000032:analysis*; D004926:Escherichia coli; D012475:Salmonella; D012477:Salmonella enteritidis; D012763:Shigella flexneri; D013056:Spectrophotometry, Ultraviolet
D004731:Endotoxins