Fractionation on lymphocyte surface antigens. I. Rapid method for eliminating labeled lipid from cell surface antigens iodinated by the lactoperoxidase catalysed reaction.
Fractionation of lactoperoxidase iodinated cell surface material on miniature DEAE-cellulose columns provided a rapid method for separating labeled lipid from cell surface antigens. The procedure also removed poorly solubilized aggregates yielding a labeled preparation which demonstrated stable, reproducible immunoprecipitation results. Using these fractionated antigens components tentatively designated as human 'T' cell specific antigens have been identified.
D000941:Antigens / Q000032:analysis*; D002462:Cell Membrane / Q000276:immunology; D005591:Chemical Fractionation; D011232:Chemical Precipitation; D002848:Chromatography, DEAE-Cellulose; D006801:Humans; D007158:Immunologic Techniques; D007455:Iodine / Q000378:metabolism*; D007784:Lactoperoxidase / Q000378:metabolism*; D008055:Lipids; D008214:Lymphocytes / Q000276:immunology*; D010544:Peroxidases / Q000378:metabolism*; D013950:Thymus Gland / Q000276:immunology
D000941:Antigens; D008055:Lipids; D007455:Iodine; D007784:Lactoperoxidase; D010544:Peroxidases