[Rapid determination of serum iron concentration using bathophenanthroline sulfonate in a formate buffered system].
A simple accurate and very reproducible procedure for measuring serum iron concentration without precipitating serum proteins is described. In the same time, iron is released from serum transferrin, reduced and determined at 535 mn by incubation in a water bath at 55 degrees for five minutes within a formate buffered system (pH 2,6; i = 0,05) including bathophenanthroline sulfonate. This method yields values very similar to those obtained by that of the International Committee for Standardization in Hematology (ICSH).
D002021:Buffers; D005561:Formates; D006801:Humans; D007202:Indicators and Reagents; D007501:Iron / Q000097:blood*; D008722:Methods; D010618:Phenanthrolines; D013053:Spectrophotometry
D002021:Buffers; D005561:Formates; D007202:Indicators and Reagents; D010618:Phenanthrolines; D007501:Iron
Détermination rapide de la sidérémie sérique à l'aide de la bathophénanthroline sulfonée en milieu formique