Characterization of feline whole-blood cultures and determination of the frequency of radiation-induced dicentrics in human and feline lymphocytes.
The Ham's F-10 and PHA culture system was applied to whole feline blood and cell-cycle characteristics such as DNA synthesis and mitotic indices were studied. The results are comparable to those obtained from human whole-blood cultures. The yields of dicentrics were also determined in lymphocytes from X-irradiated human and feline blood. The ratio between the experimental yields of dicentrics in human as compared to feline lymphocytes was 1:0.27.
D000818:Animals; D001769:Blood; D002415:Cats; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000528:radiation effects*; D004247:DNA / Q000096:biosynthesis; D004307:Dose-Response Relationship, Radiation; D005260:Female; D006801:Humans; D007399:Interphase; D008214:Lymphocytes / Q000166:cytology / Q000528:radiation effects* / Q000648:ultrastructure; D008297:Male; D008938:Mitosis / Q000528:radiation effects*; D008940:Mitotic Index / Q000528:radiation effects*; D011817:Rabbits; D014965:X-Rays
D004247:DNA