title: Solid-phase enzyme immunoassay for hepatitis B surface antigen.
abstract: A solid-phase enzyme immunoassay is described for measuring hepatitis  B surface antigen in human serum or plasma. Immunologically purified antibody labeled with horseradish peroxidase was used as the indicator. In the assay system, antibody-coated controlled-pore glass is used as a solid support and there are three sequential incubations, totaling 2 h, at room temperature. Results for serially diluted positive and reference sera compare favorably to radioimmunoassay in sensitivity and specificity.
mesh_terms: D005189:False Positive Reactions; D006514:Hepatitis B Surface Antigens / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques; D008722:Methods; D011863:Radioimmunoassay
chemical_list: D006514:Hepatitis B Surface Antigens