Quantitation of DNA repair in brain cell cultures: implications for autoradiographic analysis of mixed cell populations.
Quantitation of DNA repair in the mixed cell population of mouse embryo brain cultures has been assessed by autoradiographic analysis of unscheduled DNA synthesis following UV-irradiation. The proportion of labelled neurons and the grain density over neuronal nuclei are both less than the corresponding values for glial cells. The nuclear geometries of these two classes of cell are very different. Partial correction for the different geometries by relating grain density to nuclear area brings estimates of neuronal and glial DNA repair synthesis more closely in line. These findings have general implications for autoradiographic measurement of DNA repair in mixed cell populations and in differentiated versus dividing cells.
D000818:Animals; D001345:Autoradiography / Q000379:methods*; D001921:Brain / Q000196:embryology / Q000528:radiation effects* / Q000648:ultrastructure; D002467:Cell Nucleus / Q000648:ultrastructure; D002478:Cells, Cultured; D004260:DNA Repair; D051379:Mice; D008855:Microscopy, Electron, Scanning; D014466:Ultraviolet Rays