Detection of primitive macrophage progenitor cells in mouse bone marrow.
A previously undetected population of macrophage progenitor cells with high proliferative potential (HPP-CFC; an average of 5 x 10(4) cells/colony) in nutrient agar cultures has been demonstrated in post-fluorouracil (FU) and fluorouracil plus endotoxin (FUEt) treated and normal mouse bone marrow, using a combination of colony-stimulating factors, pregnant uterus extract (PMUE) plus human spleen-conditioned medium (HUSPCM). Neither PMUE nor HUSPCM alone stimulated colony formation by the HPP-CFC. The incidences of HPP-CFC were 1 in 2380 nucleated cells in normal marrow, 1 in 380 for 10-day post-FU, and 1 in 118 in 8-day post-FUEt marrow cells. HPP-CFC were only depleted to 57% of normal at 2 days after FU treatment, whereas the cells responsive to PMUE alone (low proliferative potential, LPP-CFC) were depleted to 1.2%, indicating a marked difference in cycling status of the respective types of progenitor cells.
D000818:Animals; D001854:Bone Marrow Cells; D002454:Cell Differentiation; D002478:Cells, Cultured; D003115:Colony-Stimulating Factors / Q000494:pharmacology; D003470:Culture Media; D005472:Fluorouracil / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D013154:Spleen; D013997:Time Factors; D014020:Tissue Extracts / Q000494:pharmacology
D003115:Colony-Stimulating Factors; D003470:Culture Media; D014020:Tissue Extracts; D005472:Fluorouracil