Target cell-substratum interaction. II. Complement-dependent antibody-induced inhibition of adherence.
When trypsinized cells were treated with alloantibody and complement they initially adhered to their substratum, then detached. Specific cytolysis did not occur. In addition to demonstrating an immunologically mediated reaction, this phenomenon has permitted the development of a new, simple, rapid assay for alloantibody that does not require a cytolytic reaction.
D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D003165:Complement System Proteins; D007111:Immunity, Cellular; D007158:Immunologic Techniques; D066298:In Vitro Techniques; D007518:Isoantibodies / Q000032:analysis; D008214:Lymphocytes / Q000276:immunology*; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000276:immunology; D014357:Trypsin / Q000494:pharmacology
D007518:Isoantibodies; D003165:Complement System Proteins; D014357:Trypsin