Quantitation of a transformation-sensitive, adhesive cell surface glycoprotein. Decrease of several untransformed permanent cell lines.
We have quantitated the transformation-sensitive, cell surface LETS glycoprotein on many untransformed cell types. By SDS-polyacrylamide gel electrophoresis, this trypsin-sensitive iodinatable glycoprotein comprises 1-3% of total cellular protein of the seven early passage cell types tested. In contrast, it constitutes less than 0.15% of the protein in four of six continuous cell lines. This decrease is reflected in alterations both in [14C]glucosamine labeling and in the immunofluorescent staining of early passage vs. these four permanent cell lines. These results help to clarify previous experiments in which CSP, a purified LETS protein, partially restored a fibroblastic phenotype to cells transformed by tumor viruses. These findings also indicate that a major decrease in this cell surface glycoprotein can occur in the establishment of a continuous cell line without resulting in cellular transformation.
D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000032:analysis; D002471:Cell Transformation, Neoplastic; D002642:Chick Embryo; D006224:Cricetinae; D005347:Fibroblasts / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D005944:Glucosamine; D006023:Glycoproteins / Q000032:analysis*; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis*; D051379:Mice
D006023:Glycoproteins; D008565:Membrane Proteins; D005944:Glucosamine