Serological characterization of human monocytes for HLA, B-lymphocyte, granulocyte, and monocyte-associated antigens by cytotoxicity testing.
Highly enriched preparations of monocytes, B and T lymphocytes, and granulocytes from 18 normal donors were serotyped in parallel in a complement-dependent cytotoxicity assay using allogeneic and heterologous antisera defining three independent tissue antigen systems. HLA and B-lymphocyte tissue antigens were detected on human monocytes although granulocyte antigens were absent. By cytotoxicity testing the presence of Ia-like antigens on monocytes was significantly diminished compared to the autologous B-lymphocyte population and has important implications in B-lymphocyte serology. The study indentified a number of human antisera obtained from multitransfused subjects and pre- and post-transplant organ recipients that were non-HLA and appeared to define monocyte-associated antigens. The serological implications of surface antigen expression on human monocytes compared with other peripheral blood cells are discussed.
D001402:B-Lymphocytes / Q000276:immunology*; D001803:Blood Transfusion; D003165:Complement System Proteins; D003601:Cytotoxicity Tests, Immunologic; D006098:Granulocytes / Q000276:immunology*; D006680:HLA Antigens / Q000032:analysis; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D007106:Immune Sera; D007519:Isoantigens / Q000032:analysis*; D009000:Monocytes / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology; D014183:Transplantation, Heterologous; D014184:Transplantation, Homologous
D006680:HLA Antigens; D007106:Immune Sera; D007519:Isoantigens; D003165:Complement System Proteins