title: Clotting factors and platelets. Immunofluorescence evidence that fibrinogen and factor VIII are present in human washed platelets, whereas prothrombin complex factors and factor XIII are lacking.
abstract: Factor I (fibrinogen) and factor VIII were shown, by an indirect immunofluorescence technique, to be present in human washed platelets. In the case of fibrinogen, the immunofluorescent pattern had a 'clod distribution' up to a 1:128 dilution of the antiserum. Factor VIII showed a 'speckled' pattern up to the 1:64 dilution. Cross-absorption studies confirmed the presence of such factors. Factor II, VII, IX, X and XIII, however, were not found in washed platelets. A diffuse fluorescence was noted using normal rabbit sera, but this was due to a cross-reaction, since it disappeared using a 1:32 diluted sera.
mesh_terms: D000818:Animals; D000941:Antigens / Q000032:analysis; D001779:Blood Coagulation Factors / Q000276:immunology*; D001792:Blood Platelets / Q000032:analysis*; D005164:Factor IX / Q000276:immunology; D005167:Factor VII / Q000276:immunology; D005169:Factor VIII / Q000276:immunology; D005170:Factor X / Q000276:immunology; D005176:Factor XIII / Q000276:immunology; D005340:Fibrinogen / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006041:Goats; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D011516:Prothrombin / Q000276:immunology; D011817:Rabbits
chemical_list: D000941:Antigens; D001779:Blood Coagulation Factors; D007106:Immune Sera; D005167:Factor VII; D011516:Prothrombin; D005169:Factor VIII; D005164:Factor IX; D005170:Factor X; D005340:Fibrinogen; D005176:Factor XIII