Determination of B lymphocyte population using antibody-coated polyacrylamide beads.
The present study describes a method for the determination of the human B cell population by the use of polyacrylamide beads which are coated with antibody specific for each of the immunoglobulin classes. This method provides a simpler determination of the B cell count than the EA or EAC rosette tests. The antibodycoated beads also have the potential of being used to quantify the subpopulations of B cells bearing specific classes of immunoglobulins.
D000178:Acrylamides; D000328:Adult; D000818:Animals; D000906:Antibodies; D001402:B-Lymphocytes / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007158:Immunologic Techniques; D007958:Leukocyte Count / Q000379:methods; D008297:Male; D008875:Middle Aged; D011108:Polymers; D012756:Sheep / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D000178:Acrylamides; D000906:Antibodies; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011108:Polymers