title: Inactivation and proteolytic cleavage of phage lambda repressor in vitro in an ATP-dependent reaction.
abstract: We have reproduced in vitro the inactivation of bacteriophage lambda repressor that occurs in vivo when a lambda lysogen is treated with agents such as ultraviolet radiation that attack DNA. ATP and a divalent cation are required for the inactivation reaction. The ind- repressor is insensitive to the inactivation mechanism. A proteolytic cleavage of repressor accompanies inactivation in vitro, as it does in vivo.
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D003090:Coliphages / Q000502:physiology*; D004926:Escherichia coli / Q000502:physiology*; D005838:Genotype; D007700:Kinetics; D008242:Lysogeny; D009154:Mutation; D014779:Virus Replication
chemical_list: D000255:Adenosine Triphosphate