Detection of H2-b and Thy-1.2 surface antigens on the differentiating murine otocyst using Nomarski optics.
The 12th postcoital day otocyst appears as a hollow cellular ball of pseudostratified columnar epithelium that has entered into its inital stages of differentiation and organogenesis. H-2b antigen was demonstrated on the ectodermally derived epithelial cells of the otocysts and on the mesodermally derived cells of the surrounding mesenchyme. Thy-1.2 antigen was detected in the mesenchymal cells, but not on the epithelial cells of the otocyst. The use of Nomarski optics as a new method for detecting cell surface staining that would otherwise be undetected by bright field optics was demonstrated.
D000818:Animals; D000954:Antigens, Surface; D004423:Ear / Q000196:embryology / Q000276:immunology*; D004622:Embryo, Mammalian / Q000276:immunology*; D005260:Female; D005865:Gestational Age; D006183:H-2 Antigens; D051379:Mice; D055095:Optics and Photonics; D011247:Pregnancy; D013601:T-Lymphocytes / Q000276:immunology*
D000954:Antigens, Surface; D006183:H-2 Antigens