omega-Oxidation of fatty acids and the acetylation p-aminobenzoic acid.
p-Aminobenzoic acid was fed to normal and alloxan-induced diabetic rats injected with [omega-14C]labeled and [2-14C]labeled fatty acids. The p-acetamidobenzoic acid that was excreted was hydrolyzed to yield acetate which was degraded. The distribution of 14C in the acetates formed when an [omega-14C]labeled fatty acid was injected was similar to that when a [2-14C]labeled fatty acid was injected. This contrasts with the finding that in acetates from 2-acetamido-4-phenylbutyric acid excreted when 2-amino-4-phenylbutyric acid was fed, there was a difference in the distributions of 14C, a difference attributable to omega-oxidation of the fatty acid. Acetylation of p-aminobenzoic acid is then concluded to occur in a different cellular environment than that of 2-amino-4-phenylbutyric acid, one in which omega-oxidation is not functional. When 2-amino-4-phenylbutyric acid was fed and [6-14C]palmitic acid injected, rather than [16-14C]palmitic acid, the distribution of 14C in acetate was the same as when [2-14C]palmitic acid was injected. This indicates that the dicarboxylic acid formed on omega-oxidation of palmitic acid does not undergo beta-oxidation to form succinyl-CoA. Thus, glucose is not formed via omega-oxidation of long-chain fatty acid.
D010129:4-Aminobenzoic Acid / Q000378:metabolism*; D000107:Acetylation; D062365:Aminobenzoates / Q000378:metabolism*; D000818:Animals; D002250:Carbon Radioisotopes; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism*; D005260:Female; D007553:Isotope Labeling; D010084:Oxidation-Reduction; D051381:Rats
D062365:Aminobenzoates; D002250:Carbon Radioisotopes; D005227:Fatty Acids; D010129:4-Aminobenzoic Acid