title: beta-D-Galactoside transport in Escherichia coli. Reversible inhibition by Aprotic Solvents and its Reconstitution in transport-negative membrane vesicles.
abstract: At relatively low concentrations (less than 3M) the aprotic solvents: dimethylsulfoxide, N-methylpyrrolidone, tetramethylurea and hexamethylphosphoric triamide, inhibited beta-D-galactoside transport by whole cells, and the derived membrane vesicles of Escherichia coli. Inhibition was not due to gross leakiness of the membrane and could be largely reversed  by a simple washing procedure...
mesh_terms: D001667:Binding, Competitive; D001693:Biological Transport, Active; D002462:Cell Membrane / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D005697:Galactosides / Q000378:metabolism*; D005796:Genes; D006027:Glycosides / Q000378:metabolism*; D007700:Kinetics; D007785:Lactose / Q000378:metabolism; D013045:Species Specificity
chemical_list: D005697:Galactosides; D006027:Glycosides; D007785:Lactose