The effects of additional flora on the response of salmonella mutants lodged in the gastrointestinal tract.
A histidine auxotroph of Salmonella typhimurium, strain TA1538, will lodge for several months in the gastrointestinal tract of otherwise germ-free rats and of rats additionally associated with bacteria characteristic of the normal flora such as Lactobacillus plantarum and Bacteroides vulgatus. In the presence of the additional flora, the concentration of strain TA1538 is diminished in the stomach but not in the lower gastrointestinal tract or in the feces. Following the ingestion of 2-nitrofluorene, there is an increase in the concentration of revertants in the feces which reflects that observed in the colon and cecum. A dose-response relationship can be demonstrated between the amount of 2-nitrofluorene ingested and the concentration of revertants in the feces. A given dose of 2-nitrofluorene, however, produces fewer revertants in the feces of rats with the additional flora than in the feces of rats associated only with strain TA1538. It is not clear whether the decreased number of revertants in the feces in the presence of the additional flora is a result of metabolic transformations of 2-nitrofluorene by B. vulgatus, which can be demonstrated in vitro, or a result of the displacement of strain TA1538 from the stomach. The rat associated with strain TA1538, or other Ames tester strains, may be useful for detecting carcinogens as mutagens within the gastrointestinal tract and for determining the influence of various constituents of the bacterial flora on the concentration of mutagenic compounds.
D000818:Animals; D001439:Bacteroides / Q000254:growth & development; D002432:Cecum / Q000382:microbiology; D003106:Colon / Q000382:microbiology; D004064:Digestive System / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D005449:Fluorenes / Q000378:metabolism / Q000494:pharmacology; D005770:Gastrointestinal Neoplasms / Q000209:etiology; D005856:Germ-Free Life; D006639:Histidine / Q000378:metabolism; D007778:Lactobacillus / Q000254:growth & development; D008297:Male; D009154:Mutation / Q000187:drug effects; D010084:Oxidation-Reduction; D051381:Rats; D012486:Salmonella typhimurium / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism
D005449:Fluorenes; D006639:Histidine