Isoleucyl-tRNA synthetase inactivation and the extent of aminoacylation of tRNAIle from Escherichia coli.
A difference in isoleucine acceptance between normal and sulfur-deficient tRNA from Escherichia coli C6 (rel-, met-, cys-) was eliminated when more isoleucyl-tRNA synthetase was added at the reaction plateau. Enzymatic deacylation was similar for both tRNAs. These results suggest that enzyme inactivation caused a premature reaction plateau which was not predicted by the rates of acylation and deacylation.
D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism*; D003545:Cysteine / Q000494:pharmacology; D004926:Escherichia coli / Q000378:metabolism*; D007533:Isoleucine-tRNA Ligase / Q000378:metabolism*; D007700:Kinetics; D013455:Sulfur / Q000172:deficiency; D046249:Transfer RNA Aminoacylation
D013455:Sulfur; D000604:Amino Acyl-tRNA Synthetases; D007533:Isoleucine-tRNA Ligase; D003545:Cysteine