Bioassay method for polyene antibiotics based on the measurement of rubidium efflux from rubidium-loaded yeast cells.
A bioassay method for the polyene antibiotics nystatin and amphotericin B is proposed based on the measurement of the efflux of rubidium ions from a rubidium-loaded yeast culture challenged with the antibiotics. For this purpose a major proportion of the intracellular K(+) ions in a Saccharomyces cerevisiae culture has been substituted by Rb(+) ions. The rubidium leakage is measured by atomic absorption spectrophotometry, and a straight-line, dose-response correlation has been obtained for both antibiotics.
D000666:Amphotericin B / Q000032:analysis*; D001681:Biological Assay / Q000379:methods*; D009761:Nystatin / Q000032:analysis*; D012413:Rubidium / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism
D009761:Nystatin; D000666:Amphotericin B; D012413:Rubidium