Reutilization of 125I-labelled anti-IgE antibody and paper discs in PRIST and RAST IgE determination.
In the paper radioimmunosorbent test (PRIST) anti-human IgE coupled paper discs are used for the estimation of total IgE in blood serum: in the radioallergosorbent test (RAST) allergen-coupled paper discs are used for the estimation of specific IgE in blood serum. The bound IgE or specific IgE is quantified by a 125I-labelled anti-human IgE. The non-bound 125I-labelled anti-human IgE can be collected and used in new assay. By a 4-h incubation of the used paper discs with 1 M glycine-HCl buffer (pH 2.7) the IgE-labelled anti-IgE complex can largely be removed. The paper discs treated in this manner can be used in a new assay.
D000888:Antibodies, Anti-Idiotypic; D001249:Asthma / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E / Q000032:analysis*; D011852:Radioallergosorbent Test / Q000379:methods; D011863:Radioimmunoassay / Q000379:methods*; D011864:Radioimmunosorbent Test / Q000379:methods
D000888:Antibodies, Anti-Idiotypic; D007073:Immunoglobulin E