Intestinal peroxisomes of goldfish (Carassius auratus)--examination for hydrolase, dehydrogenase and carnitine acetyltransferase activities.
1. Rate sedimentation and isopycnic centrifugation were used to analyse the subcellular sites of enzymes in homogenates of goldfish intestinal mucosa. 2. The results allowed the following allocations to be made: carnitine acetyl transferase-mitochondrial and peroxisomal, xanthine dehydrogenase and NAD: alpha-glycerophosphate dehydrogenase soluble phase, NADP: isocitrate dehydrogenase soluble phase and mitochondrial, and 2-naphthyl laurate hydrolase microsomal and/or brush border. 3. Histochemistry confirmed the use of alkaline phosphatase and 1-naphthyl acetate esterase as brush border and microsome markers respectively. 4. Urate oxidase, allantoinase, allantoicase, xanthine oxidase and glycollate/lactate oxidase, activities were undetectable, and 1-naphthyl palmitate hydrolase was present only as a contaminant from pancreas.
D000123:Acetyltransferases / Q000378:metabolism*; D000818:Animals; D002332:Carnitine O-Acetyltransferase / Q000378:metabolism*; D002499:Centrifugation, Density Gradient; D003530:Cyprinidae / Q000378:metabolism*; D006054:Goldfish / Q000378:metabolism*; D006651:Histocytochemistry; D006867:Hydrolases / Q000378:metabolism*; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000033:anatomy & histology / Q000201:enzymology* / Q000648:ultrastructure; D008830:Microbodies / Q000201:enzymology*; D010088:Oxidoreductases / Q000378:metabolism*
D010088:Oxidoreductases; D000123:Acetyltransferases; D002332:Carnitine O-Acetyltransferase; D006867:Hydrolases