Application of Fraunhofer diffraction theory to feature-specific detector design.
Light scatter from epithelial cells in a slit-scan flow system is modeled using the Fraunhofer condition of scalar diffraction theory. Power spectra are calculated for successive positions of model cells in the line focus of a laser beam with a Fourier transform computer program. Using the calculated power spectra, detector configurations are designed to detect specific cell structures of interest. Detector configurations are tested in a static slit-scan scatter apparatus. Data indicating the ability to detect boundaries and cell orientation are discussed.
D002467:Cell Nucleus; D003201:Computers; D003584:Cytological Techniques / Q000295:instrumentation*; D003593:Cytoplasm; D004847:Epithelial Cells; D005583:Fourier Analysis; D007834:Lasers; D055095:Optics and Photonics