title: Repression of inducible enzyme synthesis in a mutant of Escherichia coli K 12 deleted for the ptsH gene.
abstract: The genome of lambda phage with thermosensitive repressor was inserted into the pts region of the Escherichia coli chromosome. This lysogenic culture possessed the PTS1 phenotype at 30 degrees C. A mutant strain with a deletion covering the ptsH gene was isolated after a prophage curing procedure. The deletion nature of the pts mutation was confirmed in genetical and biochemical experiments. The deletion covered a small fragment of the bacterial genome not extending in the ptsI and lig genes. The isolated deltaptsH mutant possessed all characteristics of known pts mutants: pleiotropical disturbances of transport and utilization of a number of carbohydrates, repression of the enzyme inducible synthesis, and resistance to glucose catabolite repression. From these and other data we can conclude that the phosphorylated form of the heat-stable protein HPr is involved (directly or indirectly) in activation of the DNA transcription process.
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D002872:Chromosome Deletion; D003090:Coliphages; D004269:DNA, Bacterial; D004794:Enzyme Repression; D004926:Escherichia coli / Q000201:enzymology*; D005796:Genes; D008242:Lysogeny; D009154:Mutation; D010641:Phenotype; D010728:Phosphoenolpyruvate; D010770:Phosphotransferases / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D010728:Phosphoenolpyruvate; D010770:Phosphotransferases