[Conditions for S. typhimurium transfection by isolated DNA from bacteriophage P22 H5].
Transfection of spontaneous S. typhimurium LT2 WTR mutant, sensitive to bacteriophages FO, Ffm, 6SR, and resistant to bacteriophages P22 H5, C-21 and P1 vir, by DNA of P22 H5 bacteriophage in the presence of Ca2+ ions was demonstrated. The following were conditions of infection providing maximal and reproducible results: concentration of the recipient cells of 6--7x 109 cells/ml, DNA concentration of 30 microng/ml, CaC12 concentration of 0,25 M. The efficacy of transfection was 5 x 10-8.
D002122:Calcium Chloride; D004279:DNA, Viral; D012483:Salmonella Phages; D012486:Salmonella typhimurium; D014170:Transformation, Genetic
D004279:DNA, Viral; D002122:Calcium Chloride
Usloviia transfitsirovaniia S. typhimurium izolirovannoĭ DNK bakteriofaga P22 H5