Drug screening by enzyme immunoassay (EMIT) and thin-layer chromatography (Drug Skreen).
Urine samples (n = 300) were examined for drugs by thin-layer chromatography ("Drug Skreen", Brinkmann Corp.) and by the "Enzyme Multiplied Immunoassay Technique" ("Emit", Syav Corp.). The results of both methods were compared for the detection of opiates, barbiturates and amphetamines. In more than 90% of the determinations identical results were obtained with both methods. About 10% of the results of the Emit barbiturate assay differed from those of thin-layer chromatography and therefore had to be further investigated by gas liquid chromatography. It could be demonstrated that the barbiturate determination by the Emit system correlated better with the results of gas liquid chromatography. From the results of this study it is suggested that thin-layer chromatography is used as a screening test, and to confirm positive results with other methods such as Emit. If the abuse of barbiturates or opiates is suspected the corresponding Emit test should also be performed, even in cases of a negative thin-layer chromatography screening. Confirmation with a third method such as gas liquid chromatography is necessary, if thin-layer chromatography and Emit lead to divergent results.
D000662:Amphetamines / Q000652:urine*; D001463:Barbiturates / Q000652:urine*; D002855:Chromatography, Thin Layer / Q000379:methods; D006801:Humans; D007124:Immunoenzyme Techniques; D008722:Methods; D009294:Narcotics / Q000652:urine*
D000662:Amphetamines; D001463:Barbiturates; D009294:Narcotics