title: [Biological activity of extracts isolated from a virulent strain of Sh. flexneri grown in the presence of calcium ions].
abstract: The authors carried out a comparative study of the genetically connected Sh. flexneri cultures (3 virulent strains, 3 clones of an avirulent mutant selected in the flux of an oblique light from the virulent strain, and lac+ Kcp A-hybrids obtained by crossing the initial virulent cultures with the E. coli K12 Hfr strains). The absence of any correlation between the virulence of the strains under study and the lipopolysaccharide (by rhamnose) content in the extracts from them in growing the cultures in the presence of calcium ions was noted. Toxicity of the extracts from the virulent cultures was demonstrated on a model of developing chick embryos. No such property was possessed by the extracts from avirulent strains. The extracts from the virulent cultures in nontoxic doses possessed the capacity to decrease LD50 of shigella strains used for the infection. The biologically active factor determined in the extracts from the virulent cultures apparently was not lipopolysaccharide.
mesh_terms: D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002457:Cell Extracts / Q000633:toxicity*; D002642:Chick Embryo; D005796:Genes; D006824:Hybridization, Genetic; D008070:Lipopolysaccharides / Q000302:isolation & purification / Q000633:toxicity; D009154:Mutation; D011135:Polysaccharides, Bacterial / Q000302:isolation & purification / Q000633:toxicity; D012763:Shigella flexneri / Q000032:analysis / Q000187:drug effects / Q000472:pathogenicity*; D013045:Species Specificity; D014020:Tissue Extracts / Q000633:toxicity*; D014774:Virulence
chemical_list: D002457:Cell Extracts; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D014020:Tissue Extracts; D002118:Calcium
vernacular_title: Biologicheskaia aktivnost' ékstraktov, vydelennykh iz virulentnogo shtamma Sh. flexneri, vyrashchennogo v prisutstvii ionov kal'tsiia