DNA synthesis and cell survival after X-irradiation of mammalian cells treated with caffeine or adenine.
The expression of the transient depression in the rate of DNA synthesis normally observed after exposure of randomly-dividing Chinese hamster V-79 or Chinese hamster CHO cells to ionizing radiation can be postponed or diminished by a post-irradiation treatment with 1.0 to 1.0 mM adenine or 1.5 mM caffeine. Caffeine may exert its effect by creating additional sites for replication in irradiated cells. Cells treated with caffeine or adenine for 2 or 4 hours after exposure to 3000 rad of 300 kVp X-rays exhibit depressed synthesis only after the removal of caffeine or adenine. These alterations in the timing of the X-ray-induced depression of the rate of DNA synthesis have no effect on X-ray-induced cell killing. Although a 4 hour post-irradiation treatment of randomly-dividing Chinese hamster V-79 cells with 1.0 or 2.0 mM caffeine potentiates X-ray-induced cell killing, this reduction in survival is due primarily to effects on cells in S-phase.
D000225:Adenine / Q000494:pharmacology*; D002110:Caffeine / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects*; D004261:DNA Replication / Q000187:drug effects / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D013997:Time Factors; D014965:X-Rays
D002110:Caffeine; D000225:Adenine