Lymphocyte-potentiating factor from human peripheral blood adherent cells.
Low-molecular-weight factor, which can restore the diminished concanavalin A (Con A) response of adherent-cell-depleted human peripheral blood lymphocytes, was partially purified from the culture supernatant of human peripheral blood adherent cells by ion-exchange column chromatography, gel filtration and preparative paper chromatography. This factor apparently has a mechanism of action distinct from that of 2-mercaptoethanol. It affects the cells just before the onset of DNA synthesis possibly as one of the second signals in the Con-A-induced activation of human peripheral blood lymphocytes. However, it was demonstrated that the effective activation of lymphoyctes also required the presentation of Con A by adherent cells to lymphocytes at the triggering stage of the activation, indicating that adherent cells have two distinct functions in the Con-A-induced activation of lymphocytes, i.e. the the presentation of Con A to lymphoyctes and the elaboration of soluble factors.
D000818:Animals; D002448:Cell Adhesion; D002462:Cell Membrane / Q000528:radiation effects; D002852:Chromatography, Ion Exchange; D003208:Concanavalin A / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D006801:Humans; D007700:Kinetics; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D008623:Mercaptoethanol / Q000494:pharmacology; D051379:Mice; D008809:Mice, Inbred C3H; D008970:Molecular Weight; D013601:T-Lymphocytes / Q000276:immunology
D003208:Concanavalin A; D008623:Mercaptoethanol; D004247:DNA