Enzyme immunoassay of serum testosterone.
An enzyme immunoassay of serum testosterone using the testosterone-glucoamylase complex is described. Testosterone was estimated by the enzyme immunoassay after extraction with hexan: ether (4:1) for serum from men and additional thin layer chromatographic step for serum from women. The within and between assay errors, measured as the coefficient of variation were 11.1 percent (n=8) and 12.0 percent (n=12). The sensitivity of this assay was 0.25 ng. The mean testosterone concentration (+/- SD) in 19 normal men and 4 normal cycling women were 5.3 +/- 1.8 and 0.52 +/- 0.12 ng/ml, respectively. The level of testosterone found by the present assay compared favorably with those obtained by other methods.
D003429:Cross Reactions; D005260:Female; D005087:Glucan 1,4-alpha-Glucosidase; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D011863:Radioimmunoassay; D012016:Reference Values; D013739:Testosterone / Q000097:blood* / Q000276:immunology
D013739:Testosterone; D005087:Glucan 1,4-alpha-Glucosidase