title: Role of mitotic replication genes in chromosome duplication during meiosis.
abstract: In the yeast Saccharomyces cerevisiae, DNA synthesis preceding meiosis requires the expression of replication genes used during mitosis. Diploids carrying either a temperature-sensitive lesion in cdc4 (a gene required for initiation of mitotic DNA synthesis) or a lesion in cdc8 (a gene controlling mitotic polymerization) completed premeiotic DNA synthesis at a permissive but not at a restrictive temperature. The roles of cdc4 and cdc8 were evaluated by characterizing the kinetics of  premeiotic DNA synthesis after a shift to a restrictive temperature. In the cdc8 diploid, DNA synthesis was immediately inhibited, consistent with a role in polymerization. In contrast, cdc4 exhibited residual DNA synthesis characteristic of an initiation function. The cdc4 gene function was completed much earlier in the meiotic cycle than the cdc8-mediated step.
mesh_terms: D002875:Chromosomes / Q000502:physiology*; D003513:Cycloheximide / Q000494:pharmacology; D004261:DNA Replication; D005656:Fungal Proteins / Q000096:biosynthesis; D005796:Genes; D007700:Kinetics; D008540:Meiosis; D008938:Mitosis; D009154:Mutation; D014176:Protein Biosynthesis / Q000187:drug effects; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000502:physiology; D013045:Species Specificity; D013696:Temperature
chemical_list: D005656:Fungal Proteins; D003513:Cycloheximide