Enzyme multiplied immunoassay of phenobarbital by LKB reaction Rate Analyser: a comparison with analysis by gas-liquid chromatography.
A procedure for the enzyme multiplied immunoassay of phenobarbital using the LKB Reaction Rate Analyser is described. The temperature optimum, linearity, specificity, and precision of the assay are reported. A highly significant correlation coefficient is obtained when the technique is compared with gas-liquid chromatography by analysing human sera containing a range of phenobarbital levels.
D001322:Autoanalysis; D002849:Chromatography, Gas; D005069:Evaluation Studies as Topic; D005954:Glucosephosphate Dehydrogenase; D006801:Humans; D007124:Immunoenzyme Techniques; D007700:Kinetics; D007934:Leuconostoc / Q000201:enzymology; D010634:Phenobarbital / Q000097:blood*; D013696:Temperature
D005954:Glucosephosphate Dehydrogenase; D010634:Phenobarbital