Comparative studies on six blood serum glycosidases from several mammalian species.
1. Peripheral blood serum alpha-D-galactosidase, beta-D-galactosidase, beta-D-glucosidase, alpha-D-mannosidase, beta-D-xylosidase and beta-D-glucuronidase have been studied with a comparative point of view from several mammalian species: Bos taurus L. (bull), Capra hircus L. (goat), Sus scropha var. domestica L. (pig) and man. 2. Fluorimetric and spectrophotometric procedures were used for determination of enzyme activities and pH optima. 3. Glycosidase activity was generally higher with fluorescent substrates than with chromogenic substrates. 4. alpha-D-mannosidase was the most active with both fluorescent and chromogenic substrates. 5. All the studied enzymes had the same pH optimum (4.0) when the chromogenic substrates were used. 6. pH optima of these glycosidases ranged from 3.0 to 5.5 when the fluorescent substrates were used.
D000818:Animals; D002417:Cattle; D006026:Glycoside Hydrolases / Q000097:blood*; D006041:Goats; D006801:Humans; D008297:Male; D008322:Mammals / Q000097:blood*; D013045:Species Specificity; D013552:Swine
D006026:Glycoside Hydrolases