Human tumor-lymphocyte interaction in vitro. III. T lymphocytes in autologous tumor stimulation (ATS).
The T-cell nature of the responding cells in autologous tumor stimulation (ATS) was proven by (1) E-rosette formation of lymphocytes attached to tumor cells during the early period of co-cultivation; (2) blast-transformed E-rosetting cells detectable at the end of the test period (day 6) and (3) the reactivity of T-enriched lymphocyte subset and unresponsiveness of T-depleted fractions. Preparation of tumor suspensions devoid of lymphocytes made it possible to carry out cross-tests between patients. Blastogenesis with allogeneic tumors was rare. The restricted autologous nature of the reaction is a strong indication for genuine tumor-relatedness.
D000230:Adenocarcinoma / Q000276:immunology; D000293:Adolescent; D000328:Adult; D000368:Aged; D001254:Astrocytoma / Q000276:immunology; D002294:Carcinoma, Squamous Cell / Q000276:immunology; D002462:Cell Membrane / Q000276:immunology; D002478:Cells, Cultured; D002648:Child; D003429:Cross Reactions; D005260:Female; D006801:Humans; D008213:Lymphocyte Activation; D008297:Male; D008545:Melanoma / Q000276:immunology; D008875:Middle Aged; D009369:Neoplasms / Q000276:immunology*; D012516:Osteosarcoma / Q000276:immunology; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000276:immunology*