T-tubule swelling in hypertonic solutions: a freeze substitution study.
Striated muscles from Rana pipiens have been exposed for variable periods of time to Ringer solutions made hypertonic by addition of either sucrose or sodium chloride. The muscles have been rapid-frozen and then prepared for electron microscopy by either freeze-substitution, freeze-fracture or cryoultramicrotomy. The only compartment greatly affected by hypertonicity is the transverse tubular system, which is visibly swollen. None of the elements of the sarcoplasmic reticulum increase in size.
D000818:Animals; D001001:Anura; D005614:Freeze Fracturing; D006982:Hypertonic Solutions; D066298:In Vitro Techniques; D008870:Microtubules / Q000502:physiology* / Q000648:ultrastructure; D009132:Muscles / Q000502:physiology* / Q000648:ultrastructure; D011894:Rana pipiens; D012519:Sarcoplasmic Reticulum / Q000502:physiology
D006982:Hypertonic Solutions