Modified polynucleotides. I. Investigation of the enzymatic polymerization of 5-alkyl-dUTP-s.
The chemical synthesis of 5-alkyl-dUTP-s and their participation as substrates in poly[d(A-6)] primed polymerization reactions with dATP by E. coli DNA polymerase I enzyme has been described. In comparison with dTTP, at saturating substrate concentrations, the rate of hypochromic effect was found to be 17.3% higher for dUTP and was lower by 27.4% for 5-ethyl-dUTP, 29.5% for 5-n-propyl-dUTP, 31.4% for 5-n-butyl-dUTP and by 85.0% for 5-n-pentyl-dUTP. No hypochromic effect could be observed, however, with 5-iso-propyl-, 5-tert.butyl- and 5-n-hexyl-dUTP-s. Polydeoxynucleotides have also been isolated from the reaction mixture and some of their structural properties determined.
D004256:DNA Polymerase I; D003856:Deoxyuracil Nucleotides / Q000138:chemical synthesis; D004926:Escherichia coli / Q000201:enzymology; D008722:Methods; D011089:Polydeoxyribonucleotides / Q000096:biosynthesis*; D013056:Spectrophotometry, Ultraviolet; D013329:Structure-Activity Relationship
D003856:Deoxyuracil Nucleotides; D011089:Polydeoxyribonucleotides; D004256:DNA Polymerase I