title: Proteinase mutants of Saccharomyces cerevisiae.
abstract: Fifty-nine mutants with reduced ability to cleave the chymotrypsin substrate N-acetyl-DL-phenylalanine beta-naphthyl ester have been isolated in S. cerevisiae. All have reduced levels of one or more of the three well-characterized proteinases in yeast. All have reduced levels of proteinase C (carboxy-peptidase Y). These mutations define 16 complementation groups.
mesh_terms: D003433:Crosses, Genetic; D004591:Electrophoresis, Polyacrylamide Gel; D005796:Genes; D008040:Genetic Linkage; D009154:Mutation; D009281:Naphthalenes; D010447:Peptide Hydrolases / Q000378:metabolism*; D010649:Phenylalanine / Q000031:analogs & derivatives / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
chemical_list: D009281:Naphthalenes; C013791:N-acetylphenylalanine beta-naphthyl ester; D010649:Phenylalanine; D010447:Peptide Hydrolases