Effects of in vivo priming on in vitro induction of cytotoxicity. I. Non-specific augmentation by in vivo presensitization with allogeneic or xenogeneic cells.
In vivo presensitization of donor mice of responding cells with third party cellular antigens augmented in vitro generation of cytotoxic T lymphocytes in allogeneic and xenogeneic combinations. In vitro induction of detectable cytotoxicity in presensitized responding cells required the incubation period needed for in vitro primary response. However, such cytotoxic T lymphocytes were generated after in vitro stimulation with monolayers of methylcholanthrene-induced tumor cells, UV-irradiated or heated spleen cells which had proved to be effective in secondary but not in primary response. Presensitized responding cells exposed to 600R-irradiation did not augment in vitro induction of cytotoxicity in normal responding cells. The augmenting effect of presensitized responding cells may be attributable to radiosensitive T cells which are in a transitional state in differentiation from typical unprimed cells to typical primed cells.
D000818:Animals; D000951:Antigens, Neoplasm / Q000276:immunology*; D046508:Culture Techniques; D003602:Cytotoxicity, Immunologic; D005260:Female; D006358:Hot Temperature; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009368:Neoplasm Transplantation; D051381:Rats; D012513:Sarcoma, Experimental / Q000276:immunology*; D013154:Spleen; D013601:T-Lymphocytes / Q000276:immunology* / Q000528:radiation effects; D014466:Ultraviolet Rays
D000951:Antigens, Neoplasm