Normal erythropoietic helper T cells in congenital hypoplastic (Diamond-Blackfan) anemia.
To examine the erythropoietic function of T and null cells in congenital hypoplastic (Diamond-Blackfan) anemia, we fractionated the peripheral blood of three normal subjects and three affected patients into subclasses of null, T and B cells. Mixtures of these cells were co-cultured in plasma clots in the presence of erythropoietin. Erythroid colonies grew in cultures of normal null cells if either normal or patient T cells were co-cultured with them. Null cells of patients with hypoplastic anemia did not produce erythroid colonies under any culture conditions. We conclude that in this disorder, T cells function normally as helper cells in erythropoiesis and do not suppress colony formation, whereas the erythroid progenitor cells in the peripheral blood null-cell fractions are deficient in either number of function.
D000293:Adolescent; D000741:Anemia, Aplastic / Q000097:blood / Q000151:congenital* / Q000503:physiopathology; D001402:B-Lymphocytes; D002648:Child; D004920:Erythropoiesis; D006801:Humans; D008211:Lymphocyte Cooperation; D008297:Male; D012397:Rosette Formation; D013577:Syndrome; D013601:T-Lymphocytes / Q000502:physiology