[Autoantigen T in guinea pig germinal cells: fine localization and ultrastructural lesions induced in vitro by specific anti-T autoantibody and complement].
Four autoantigens (S, P, T, Z), are known to be present in guinea pig spermatozoa. The only anti-T antibody is able to fix complement and is spermotoxic. Using immunoenzymatic technics, autoantigen T has been localized on the plasma membrane of spermatozoa and spermatids. A quantitative ultrastructural study has shown the anti-T induced irreversible, specific lesions on the germinal cells in presence of complement. A few minutes after addition of complement, almost all the cells are injured. Control sere (normal serum, anti-ova or anti-S or anti-P sera are inefficient. These findings are related to the mechanisms of autoimmune aspermatogenetic orchitis.
D000818:Animals; D000918:Antibody Specificity; D000941:Antigens / Q000032:analysis*; D001323:Autoantibodies; D001324:Autoantigens / Q000032:analysis*; D003165:Complement System Proteins / Q000378:metabolism*; D006168:Guinea Pigs; D007124:Immunoenzyme Techniques; D008297:Male; D013094:Spermatozoa / Q000276:immunology* / Q000648:ultrastructure
D000941:Antigens; D001323:Autoantibodies; D001324:Autoantigens; D003165:Complement System Proteins
L'autoantigène T des cellules germinales de cobaye: localisation par immunoperoxydase et lésions ultrastructurales induites in vitro par l'autoanticorps qui lui est spécifique en présence du complément