Mitogenic stimulation of murine spleen cells: relation to susceptibility to Salmonella infection.
The screening of several inbred strains of mice suggested that the capacity of their spleen cells to respond to the mitogenic effect of lipopolysaccharide (LPS) of gram-negative bacteria was correlated with their resistance to intraperitoneal infection with Salmonella typhimurium. An infection of LPS into mice caused changes in the in vitro responsiveness of their spleen cells to the mitogenic effects of LPS and phytohemagglutinin. Pretreatment of mice with whole ultraviolet (UV)-killed bacteria led to a marked rise in the in vitro response of the spleen cells to UV-killed bacteria, but not to LPS or or phytohemagglutinin. This enhanced response to UV-killed bacteria was not specific for the O antigens of the bacteria.
D000818:Animals; D004306:Dose-Response Relationship, Immunologic; D004731:Endotoxins / Q000633:toxicity; D037102:Lectins / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008213:Lymphocyte Activation; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D011135:Polysaccharides, Bacterial / Q000494:pharmacology; D012480:Salmonella Infections / Q000276:immunology*; D012486:Salmonella typhimurium / Q000276:immunology* / Q000472:pathogenicity / Q000528:radiation effects; D013154:Spleen / Q000166:cytology*; D014466:Ultraviolet Rays
D004731:Endotoxins; D037102:Lectins; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial