[Isolation and properties of merodiploid strains with F-factor including the pts-region of the Escherichia coli K-12 chromosome].
A technique of hybridization of haploid methanol-utilizing yeast Pichia pinus MH4 is worked out using UV- and N-nitrosoguanidine-induced auxotrophic mutants. Vegetative diploid cultures are isolated. Tetrad analysis and random spore analysis have revealed a meiotic nature of spores, recombination of genetic material in the process of sporulation and the chromosomal nature of some mutations. A possibility to construct a genetic map of the yeast Pichia pinus MH4 is demonstrated on the basis of tetrad analysis. Three linkage groups are revealed. The life cycle in a homothalic haploid yeast, Pichia pinus, was demonstrated. They are capable to form zygotes and meiotic spores under conditions preventing vegetative growth.
D001692:Biological Transport; D050260:Carbohydrate Metabolism; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D003227:Conjugation, Genetic; D004171:Diploidy; D004926:Escherichia coli; D005144:F Factor; D005696:Galactosidases / Q000096:biosynthesis; D008353:Mannitol / Q000378:metabolism; D010770:Phosphotransferases / Q000096:biosynthesis; D011995:Recombination, Genetic; D013045:Species Specificity
D008353:Mannitol; D010770:Phosphotransferases; D005696:Galactosidases
Vydelenie i svoĭstva marodiploidnykh shtammov s F'-faktorom, vkliuchaiushchim pts-oblast' khromosomy Escherichia coli K-12.