A non-enzymic procdure for the quantitative analysis of (3-methoxy-4-sulphoxyphenyl)ethylene glycol(MHPG sulphate) in human urine using stable isotope dilution and gas chromatography-mass spectrometry.
A method is described for the quantitative analysis of (3-methoxy-4-sulphoxyphenyl)-ethylene glycol (MHPG sulphate) in human urine, based on selected ion monitoring gas chromatography--mass spectrometry and using a specifically deuterium-labelled analogue of MHPG sulphate as internal standard. The procedure involves extraction of the urine sample on Amberlite XAD-2, followed by isolation of MHPG sulphate by column chromatography on Sephadex LH-20. Cleavage of the sulphate conjugate and formation of the MHPG tris(trifluoroacetate) derivative are carried out in a one-step reaction, without recourse to enzymic hydrolysis.
D002849:Chromatography, Gas; D006018:Glycols / Q000652:urine*; D006801:Humans; D013058:Mass Spectrometry; D008722:Methods; D008734:Methoxyhydroxyphenylglycol / Q000652:urine*; D011865:Radioisotope Dilution Technique
D006018:Glycols; D008734:Methoxyhydroxyphenylglycol