[Albumin secreting hepatocytes detected as plaque-forming cells in liquid medium].
Albumin-secretins hepatocytes were detected as plaque-forming cells when incubated in a complete culture medium containing sheep eythrocytes coated  with purified anti-albumin, plus anti-albumin serum and complement added at appropriate dilutions. Plaque-formation is observed after incubation at 37 degrees C for 1 h 1/2 between two properly spaced microscope slides sealed with paraffin. Plaque enumeration revealed that 17-18% of normal adult rat lover cells secreted albumin. The assay which is reproductible and highly sensitive is also done in a single step.
D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D006462:Hemolytic Plaque Technique; D008099:Liver / Q000378:metabolism*; D051381:Rats; D012709:Serum Albumin / Q000378:metabolism*
D003470:Culture Media; D012709:Serum Albumin
Détection d'hépatocytes sécréteurs d'albumine par formation de plages d'hémolyse en milieu lequide