Characterization of human lymphocyte subpopulations for cytotoxicity against tumor-derived monolayer cultures.
Lymphocyte fractions of three healthy donors were tested for cytotoxicity in a 48-h assay on several tumor-derived lines. Analysis of surface markers indicated that a non-T non-B fraction comprising about 50% Fc-receptor-positive cells was most potent. The cell yield in this fraction was on the average 6.5% of the non-fractionated population. Elimination of Slg positive cells did not influence the cytotoxic potential. Pure T cells isolated with E-rosetting subsequent to passage on nylon wool column had no or very low low cytotoxicity. We had indications that cells without conventional markers--"null" cells--were also cytotoxic.
D002460:Cell Line; D002478:Cells, Cultured; D003601:Cytotoxicity Tests, Immunologic; D006801:Humans; D007141:Immunoglobulin Fc Fragments; D007158:Immunologic Techniques; D008214:Lymphocytes / Q000276:immunology*; D009369:Neoplasms / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
D007141:Immunoglobulin Fc Fragments