Sensitization of resistant myeloid leukemia clone cells by anti-cancer drugs to factor-stimulating differentiation.
Studies were made on the effect of cancer chemotherapeutic drugs on in vitro differentiation of a clone (R4) of mouse myeloid leukemic cells (MI) that is resistant to inducers. Treatment of the cells with 50% ascitic fluid (an inducer) plus 0.25 microgram/ml of adriamycin or 0.3 microgram/ml of daunomycin induced phagocytic activity and suppressed cell growth, but had little effect on cell viability; treatment with ascitic fluid or the drugs alone had no effect. In combination with ascitic fluid, mitomycin-C, hydroxyurea, 5-fluorouracil, or bleomycin also induced phagocytic activity, but 6-mercaptopurine, amethopterin, or aminopterin did not. These drugs also induced other differentiation-associated properties, lysozyme activity, and locomotive activity. The present results indicate that some cancer chemotherapeutic drugs sensitive resistant leukemic cells to an inducer of cell differentiation.
D000970:Antineoplastic Agents / Q000494:pharmacology*; D001202:Ascitic Fluid / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D002999:Clone Cells / Q000187:drug effects*; D003115:Colony-Stimulating Factors; D003630:Daunorubicin / Q000494:pharmacology; D004317:Doxorubicin / Q000494:pharmacology; D005472:Fluorouracil / Q000494:pharmacology; D066298:In Vitro Techniques; D007951:Leukemia, Myeloid / Q000201:enzymology / Q000276:immunology* / Q000473:pathology; D008727:Methotrexate / Q000494:pharmacology; D008937:Mitomycins / Q000494:pharmacology; D009113:Muramidase / Q000378:metabolism; D010587:Phagocytosis
D000970:Antineoplastic Agents; D003115:Colony-Stimulating Factors; D008937:Mitomycins; D004317:Doxorubicin; D009113:Muramidase; D005472:Fluorouracil; D008727:Methotrexate; D003630:Daunorubicin