[Isolation of flagellin antigens of salmonellae by the method of ultracentrifugation and their use for preparation of erythrocytic H-diagnostic agents].
Flagellar preparations were obtained from 6 salmonellae strains by differential ultracentrifugation; they were characterized by morphological, immunochemical, and serological tests. The results of investigations showed that the preparations possessed high serological activity; somatic antigen admixture was insignificant. Flagellins extracted from the flagellae were used to prepare erythrocytic H-diagnostic agents. The results of their use in the examination of sera of healthy persons, and of those suffering from typhoid fever and salmonellosis indicated the expediency of using passive H-hemagglutination for diagnostic purposes.
D000942:Antigens, Bacterial / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000276:immunology*; D046508:Culture Techniques; D004912:Erythrocytes / Q000276:immunology; D005408:Flagellin / Q000276:immunology*; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D008854:Microscopy, Electron; D012475:Salmonella / Q000276:immunology*; D012480:Salmonella Infections / Q000175:diagnosis; D012485:Salmonella typhi; D012486:Salmonella typhimurium; D014435:Typhoid Fever / Q000175:diagnosis; D014461:Ultracentrifugation
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D005408:Flagellin
Vydelenie zhgutikovykh antigenov sal'monell metodom ul'tratsentrifugirovaniia i ispol'zovanie ikh dlia prigotovleniia eritrotsitarnykh H-diagnostikumov.