[alpha-Amylase activity in lysosomes of Aspergillus oryzae (author's transl)].
The alpha-amylase of mycelial cells of Aspergillus oryzae exists in a particular form in 8000 g pellet. The lysosomal localization of acid phosphatase is confirmed by electron microscopy. The purification of lysosomes by discontinuous gradient of sucrose in D2O shows that alpha-amylase activity is bound to these particles.
D000135:Acid Phosphatase / Q000032:analysis; D000681:Amylases / Q000302:isolation & purification*; D001230:Aspergillus / Q000201:enzymology*; D001236:Aspergillus oryzae / Q000201:enzymology*; D008247:Lysosomes / Q000201:enzymology; D000516:alpha-Amylases / Q000302:isolation & purification*
D000135:Acid Phosphatase; D000681:Amylases; D000516:alpha-Amylases
Mise en évidence d'une activité alpha-amylasique dans les lysosomes d'Aspergillus oryzae.