Immunofluorescent localization and ultrastructural characterization of gonadotrophe cells in the adenohypophysis of the barbary drake (Cairina moschata L.) using anti-chicken LH serum.
An indirect immunofluorescence technique and an anti-chicken LH serum were used to localize cells in the adenohypophyses of drakes at different stages of their breeding cycle, after castration, and after castration combined with thyroxine treatment. Immunofluorescent cells were distributed throughout both lobes of the adenohypophyses from control and experimental birds and were shown to be alcian blue positive. PAS negative, basophiles. Immunofluorescent cells were as numerous in castrated birds as in castrated birds treated with thyroxine. Adjacent thin and semi-thin sections were used to study the cells binding anti-LH serum at light microscope and ultrastructural levels. The cells contained spherical granules with variable densities and diameters ranging between 40 and 280 nm in the rostral (=cephalic) lobe, and between 60 and 260 nm in the caudal lobe. The light microscope and ultrastructural observations showed that the anti-LH serum binds to cells which have been classified by other authors in the Pekin duck, quail and pigeon as TSH producing delta cells. The experimental technique used did not permit a distinction to be made between cells producing FSH and LH.
D000818:Animals; D000906:Antibodies; D002369:Castration; D002645:Chickens / Q000276:immunology; D004372:Ducks / Q000502:physiology*; D005455:Fluorescent Antibody Technique; D006065:Gonadotropins, Pituitary / Q000378:metabolism; D007986:Luteinizing Hormone / Q000276:immunology; D008297:Male; D008854:Microscopy, Electron; D010902:Pituitary Gland / Q000166:cytology*; D010903:Pituitary Gland, Anterior / Q000166:cytology*; D013974:Thyroxine / Q000494:pharmacology
D000906:Antibodies; D006065:Gonadotropins, Pituitary; D007986:Luteinizing Hormone; D013974:Thyroxine