title: Membrane lipid metabolism of Bacillus Calmette-Guerin-induced rabbit alveolar macrophages.
abstract: We examined the uptake of radiolabeled lysophospholipids and oleic acid by Bacillus Calmette-Guerin-induced rabbit alveolar macrophages either in the presence or absence of challenge particles. There was no difference in the uptake and metabolism of lysophospholipids by control or challenged cells for incubation periods up to 5 h. When incubated with [3H]oleic acid, challenged cells consistently exhibited a slightly greater uptake of radioactivity. Extraction of the whole cells revealed that the greater amount of radioactivity found in the challenged cells primarily was in triacylglycerol. There was no marked difference in the amount of radioactivity associated with the phospholipids in the whole cell extracts from control and challenged cells. When the macrophages were pre-labeled for 15 min with [3H]oleic acid and then reincubated in fresh medium in the presence or absence of autoclaved Escherichia coli B, more radioactivity was retained by the challenged cells, again in the form of triacylglycerol. Only in isolated plasma membrane fractions did we observe a difference in the amount of radioactivity associated with phospholipids from control and challenged cells. Plasma membranes isolated from Bacillus Calmette-Guerin-induced rabbit alveolar macrophages that had been incubated for 6 h with [3]oleic acid in the presence of E. coli B contained significantly higher level of radioactivity in all lipids than plasma membranes from control cells. Since the greatest and the most consistent difference between control and challenged cells is associated with the triacylglycerol molecule, it is postulated that this molecule may serve as a precursor in the synthesis of alveolar macrophage phospholipids, both by the reacylation pathway and the de novo pathway. It is possible that the high level of radiolabeled phospholipid found in the plasma membrane arose via the de novo pathway following the cleavage of an acyl group as we have found cytidine diphosphocholine phosphotransferase in the plasma membrane fraction (Wang, P., DeChatelet, L.R., and Waite, M. (1977) Biochim. Biophys. Acta 450, 311--321).
mesh_terms: D000818:Animals; D001500:BCG Vaccine; D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism; D004926:Escherichia coli; D005227:Fatty Acids / Q000378:metabolism; D005260:Female; D005989:Glycerides / Q000096:biosynthesis; D007700:Kinetics; D008264:Macrophages / Q000378:metabolism*; D008563:Membrane Lipids / Q000378:metabolism*; D009163:Mycobacterium bovis; D009829:Oleic Acids / Q000378:metabolism; D010743:Phospholipids / Q000096:biosynthesis; D011650:Pulmonary Alveoli; D011817:Rabbits; D014280:Triglycerides / Q000096:biosynthesis
chemical_list: D001500:BCG Vaccine; D005227:Fatty Acids; D005989:Glycerides; D008563:Membrane Lipids; D009829:Oleic Acids; D010743:Phospholipids; D014280:Triglycerides