Cellular components of allograft rejection: identity, specificity, and cytotoxic function of cells infiltrating acutely rejecting allografts.
Functioning mononuclear cells have been harvested from heterotopic rat cardiac allografts during maximal transplant cellular infiltration. T cells, identified by a T cell-specific absorbed rabbit anti-rat brain serum, constituted two-thirds of the total cells recovered. Approximately 20% of the infiltrating cells bear and synthesize surface immunoglobulin. Macrophages, identified by latex ingestion and morphologic and cytochemical techniques, comprise 9% of the graft infiltrate. Donor-specific cytotoxic T lymphocytes are concentrated within the graft. A separate population of Fc receptor-positive recovered cells mediate antibody-dependent LMC (Ab-LMC). Neither effector cell was adherent or phagocytic. These studies have conclusively established that cytotoxic T lymphocytes accumulate within rejecting allografts; however, the enriched presence of cytotoxic T cells within the grafts is not fully dependent upon antigen recognition per se, since Lew animals grafted with both BN and BUF hearts have Lew anti-BN and Lew anti-BUF killer cells in each graft.
D000818:Animals; D001921:Brain / Q000276:immunology; D002448:Cell Adhesion; D002462:Cell Membrane / Q000276:immunology; D002465:Cell Movement; D002469:Cell Separation; D003601:Cytotoxicity Tests, Immunologic; D006084:Graft Rejection; D016027:Heart Transplantation; D007106:Immune Sera / Q000494:pharmacology; D007111:Immunity, Cellular; D007158:Immunologic Techniques; D008214:Lymphocytes / Q000276:immunology*; D010586:Phagocytes; D051381:Rats; D011914:Rats, Inbred BN; D011915:Rats, Inbred BUF; D011917:Rats, Inbred Lew; D011947:Receptors, Antigen, B-Cell / Q000096:biosynthesis; D013045:Species Specificity; D013997:Time Factors; D014184:Transplantation, Homologous
D007106:Immune Sera; D011947:Receptors, Antigen, B-Cell