title: Two new bioassay techniques for nitrofurans: Bacteroides fragilis and rec-Escherichia coli as indicator strains.
abstract: Techniques for the microbiological assay of nitrofurans are described. In one the indicator strain is Bacteroides fragilis; use of this system enables concentrations of nifuratel and furazolidone of down to 0.7 micrograms/ml to be estimated. The other system uses a recombination-deficient (recA-uvrA-) mutant of Escherichia coli; with this indicator, concentrations of 0.2 micrograms/ml, or less, of five nitrofurans can be assayed.
mesh_terms: D001431:Bacteriological Techniques; D001441:Bacteroides fragilis / Q000187:drug effects*; D001681:Biological Assay / Q000379:methods*; D004926:Escherichia coli / Q000187:drug effects*; D009581:Nitrofurans / Q000032:analysis*
chemical_list: D009581:Nitrofurans