Separation of epidermal layers of the newborn rat.
A method is presented for the separation of epidermal strata by the successive elimination of either the basal or basal and spinous cells with 0.24 M NH4Cl at pH 9.5. Histologic evidence suggests that the residual epidermal strata obtained after incubation of the skin with NH4Cl are reproducible; hence, this technique circumvents loss of granular layer histidine-rich protein inherent with trypsin separation and provides an effective procedure for biochemical analysis of arginine-rich and lysine-rich proteins in the various differentiating epidermal cells.
D000643:Ammonium Chloride / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn; D005069:Evaluation Studies as Topic; D006651:Histocytochemistry; D006652:Histological Techniques; D011506:Proteins / Q000032:analysis; D051381:Rats; D012867:Skin / Q000032:analysis / Q000187:drug effects; D014357:Trypsin / Q000494:pharmacology
D011506:Proteins; D000643:Ammonium Chloride; D014357:Trypsin