[Reparation of UV-damaged bacteriophage ed].
A repair of UV-damaged phage DNA in the "phage-host" system in accordance with the excision reparative mechanism is demonstrated by means of centrifugation in alkaline sucrose gradient of virulent 3H-thymidine labelled phage sd. The increase of the transfectants quantity of UV-irradiated DNA on uvr+ bacteria compatibly to uvr- bacteria evidences that the bacterial host participates in phage reparation. Caffeine inhibition of UV-irradiated phage sd survival confirms the participation of cell-host in reparation of UV-damaged phage.
D002110:Caffeine / Q000494:pharmacology; D003090:Coliphages / Q000187:drug effects / Q000528:radiation effects*; D004260:DNA Repair / Q000528:radiation effects*; D004279:DNA, Viral / Q000235:genetics / Q000528:radiation effects*; D004926:Escherichia coli / Q000235:genetics / Q000528:radiation effects; D008970:Molecular Weight; D009154:Mutation / Q000528:radiation effects; D014162:Transfection / Q000528:radiation effects; D014466:Ultraviolet Rays
D004279:DNA, Viral; D002110:Caffeine
Reparatsiia UF-povrezhdeniĭ bakteriofaga ed.