Maintenance procedures for the curtailment of genetic instability: Xanthomonas campestris NRRL B-1459.
Characteristics are described of small-colony variants of Xanthomonas campestris NRRL B-1459 which are frequently encountered when routine culture maintenance procedures are employed. In contrast to the parental type, smallcolony variants were shown to be resistant to a number of antibiotics, to acridine orange, and to phage which are virulent for the parent colony type. Sensitivity to ultraviolet radiation was similar in both colony types. A simple method for preservation of viable cells is described. The suitability of the method for providing reproducible inocula free from variant cell types is examined.
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001431:Bacteriological Techniques; D004352:Drug Resistance, Microbial; D014644:Genetic Variation; D006088:Gram-Negative Aerobic Bacteria / Q000187:drug effects / Q000254:growth & development*; D011309:Preservation, Biological
D000900:Anti-Bacterial Agents