Functionally important arginine residues of aspartate transcarbamylase.
The reaction of phenylglyoxal with aspartate transcarbamylase and its isolated catalytic subunit results in complete loss of enzymatic activity (Kantrowitz, E. R., and Lipscomb, W. N. (1976) J. Biol. Chem. 251, 2688-2695). If N-(phosphonacetyl)-L-aspartate is used to protect the active site, we find that phenylglyoxal causes destruction of the enzyme's susceptibility to activation by ATP and inhibition by CTP. Furthermore, CTP only minimally protects the regulatory site from reaction with this reagent. The modified enzyme still binds CTP although with reduced affinity. After reaction with phenylglyoxal, the native enzyme shows reduced cooperativity. The hybrid with modified regulatory subunits and native catalytic subunits exhibits slight heterotropic or homotropic properties, while the reverse hybrid, with modified catalytic subunits and native regulatory subunits, shows much reduced homotropic properties but practically normal heterotropic interactions. The decrease in the ability of CTP to inhibit the enzyme correlates with the loss of 2 arginine residues/regulatory chain (Mr = 17,000). Under these reaction conditions, 1 arginine residue is also modified on each catalytic chain (Mr = 33,000). Reaction rate studies of p-hydroxymercuribenzoate, with the liganded and unliganded modified enzyme suggest that the reaction with phenylglyoxal locks the enzyme into the liganded conformation. The conformational state of the regulatory subunit is implicated as having a critical role in the expression of the enzyme's heterotropic and homotropic properties.
D000447:Aldehydes / Q000031:analogs & derivatives; D001120:Arginine / Q000378:metabolism*; D001221:Aspartate Carbamoyltransferase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D001665:Binding Sites / Q000187:drug effects; D055598:Chemical Phenomena; D002621:Chemistry; D003597:Cytosine Nucleotides / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D004926:Escherichia coli / Q000201:enzymology; D006037:Glyoxal / Q000031:analogs & derivatives; D006902:Hydroxymercuribenzoates / Q000378:metabolism; D007700:Kinetics; D008024:Ligands; D046911:Macromolecular Substances; D009943:Organophosphorus Compounds / Q000494:pharmacology; D010746:Phosphonoacetic Acid / Q000031:analogs & derivatives; D011485:Protein Binding / Q000187:drug effects; D011487:Protein Conformation / Q000187:drug effects
D000447:Aldehydes; D003597:Cytosine Nucleotides; D006902:Hydroxymercuribenzoates; D008024:Ligands; D046911:Macromolecular Substances; D009943:Organophosphorus Compounds; D001224:Aspartic Acid; D006037:Glyoxal; C013195:sparfosic acid; D001120:Arginine; D001221:Aspartate Carbamoyltransferase; D010746:Phosphonoacetic Acid