title: alpha-Amylase from human pancreatic juice as an electrophoretically pure isozyme.
abstract: Human pancreatic juice collected antiseptically and strictly free from other body fluids and juices, was examined for the isozyme composition of alpha-amylase. Unlike those of the extract of and exudate from pancreatic tissue, the composition of alpha-amylase isozyme in the secreted juice was simple and consisted of only two isozymes, a major one and a minor one, and the reason for difference in the isozyme composition is discussed. The major alpha-amylase isozyme was obtained in a crystalline state with a molecular weight of 5.4-5.5 X 10(4).
mesh_terms: D000681:Amylases / Q000302:isolation & purification*; D007527:Isoenzymes / Q000302:isolation & purification*; D010189:Pancreatic Juice / Q000201:enzymology*; D000516:alpha-Amylases / Q000302:isolation & purification*
chemical_list: D007527:Isoenzymes; D000681:Amylases; D000516:alpha-Amylases