Immunochemical determination of ganglioside GM2, by inhibition of complement-dependent liposome lysis.
Immunochemical quantitative determination of a lipid antigen, ganglioside GM2, has been developed, based on the inhibition of the immune lysis of liposomes containing the antigen in their lipid bilayer. It has been shown that the full expression of the antigenicity of the competing lipid requires its dispersion in accessory lipids. The assay of inhibition of liposome lysis can be used also for the establishment of the antigenic similarity of structurally related lipid antigens.
D000818:Animals; D000917:Antibody Formation; D002554:Cerebrosides / Q000276:immunology; D002784:Cholesterol / Q000378:metabolism; D003165:Complement System Proteins / Q000494:pharmacology*; D003429:Cross Reactions; D004306:Dose-Response Relationship, Immunologic; D005455:Fluorescent Antibody Technique; D005678:G(M2) Ganglioside / Q000032:analysis* / Q000494:pharmacology; D005732:Gangliosides / Q000032:analysis*; D006168:Guinea Pigs; D006461:Hemolysis; D006801:Humans; D008081:Liposomes / Q000276:immunology*; D010713:Phosphatidylcholines / Q000378:metabolism; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000276:immunology; D013433:Sulfoglycosphingolipids / Q000276:immunology
D002554:Cerebrosides; D005732:Gangliosides; D008081:Liposomes; D010713:Phosphatidylcholines; D013433:Sulfoglycosphingolipids; D005678:G(M2) Ganglioside; D012710:Serum Albumin, Bovine; D003165:Complement System Proteins; D002784:Cholesterol