An approach to simultaneous immunofluorescent and autoradiographic analysis of human normal and neoplastic blood cells.
A technique for evaluating membrane immunofluorescence and isotope (3H-Thymidine, 3H-Uridine, 3H-Leucine) labelling in the same cell is described in detail. The possible interference of autoradiographic labelling on the fluorescent staining have been considered and found not to alter the final preparations. The technique proved valid both in normal and in neoplastic cells irrespective of their origin (peripheral blood, bone marrow, lymph node). Its possible extensions are finally discussed.
D000328:Adult; D001345:Autoradiography; D001853:Bone Marrow; D005455:Fluorescent Antibody Technique; D006801:Humans; D007244:Infectious Mononucleosis / Q000097:blood / Q000276:immunology; D007930:Leucine / Q000378:metabolism; D007938:Leukemia / Q000097:blood / Q000276:immunology*; D008198:Lymph Nodes; D008214:Lymphocytes / Q000276:immunology* / Q000378:metabolism*; D008223:Lymphoma / Q000097:blood / Q000276:immunology*; D013936:Thymidine / Q000378:metabolism; D014529:Uridine / Q000378:metabolism
D007930:Leucine; D013936:Thymidine; D014529:Uridine