Cytochemical and ultrastructural characters of human peripheral blood lymphocytes according to their surface markers.
The human peripheral blood lymphocytes are characterized by different surface markers: The B lymphocytes by the EAC rosettes and surface immunoglobulin and the T lymphocytes by the E-rosettes. Each lymphoid population has been studied for cytochemical parameters (Acid phosphatase, beta-glucuronidase) by light microscopy and for ultrastructural characteristics by micromanipulation and immunoelectronmicroscopy. It has been shown that the studied enzymes are markers for T cells with high affinity for sheep red blood cells and that the separation of lymphocytes by the rosette techniques leads to homogenous cell populations at the ultrastructural level. A classification of peripheral lymphocytes according to their immunocytochemical profile is discussed.
D000135:Acid Phosphatase / Q000097:blood; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology; D005966:Glucuronidase / Q000097:blood; D006801:Humans; D008214:Lymphocytes / Q000201:enzymology / Q000276:immunology* / Q000648:ultrastructure; D011947:Receptors, Antigen, B-Cell / Q000276:immunology*; D012397:Rosette Formation; D012756:Sheep / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D011947:Receptors, Antigen, B-Cell; D000135:Acid Phosphatase; D005966:Glucuronidase