Synthesis of oligonucleotides with sequences identical with or analogous to the 3'-end of 16S ribosomal RNA of Escherichia coli: preparation of m-6-2-A-C-C-U-C-C and A-C-C-U-C-m-4-2C via phosphotriester intermediates.
The synthesis of two fully-protected hexanucleotides (11a and 11b) via a phosphotriester approach, which is based on the use of two types of protecting groups for the internucleotide linkages, i.e. one 2,2,2-tribromo-ethyl at the 5'-terminus and four 2-chlorophenyl groups for the remaining linkages, is reported. The hexanucleotides 11a and 11b, assembled via a block-wise two-step phosphotriester method, can be deblocked conveniently to give the two hexamers 12a and 12b containing only 3'leads to5' internucleotide linkages.
D000227:Adenine Nucleotides; D001483:Base Sequence; D055598:Chemical Phenomena; D002621:Chemistry; D003597:Cytosine Nucleotides; D004926:Escherichia coli; D009841:Oligonucleotides / Q000138:chemical synthesis*; D009843:Oligoribonucleotides / Q000138:chemical synthesis*; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D014500:Uracil Nucleotides
D000227:Adenine Nucleotides; D003597:Cytosine Nucleotides; D009841:Oligonucleotides; D009843:Oligoribonucleotides; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D014500:Uracil Nucleotides