Macrophage requirements of PPD-induced blastogenic factor production by T and B lymphocytes in the guinea-pig.
An attempt has been made to characterize the cell type which releases blastogenic factor(s) (BF) on purified protein derivative (PPD) stimulation in complete Freund's adjuvant (CFA)-sensitized guinea-pig system and, further, to examine whether macrophages (Mphi) are required for antigen activation of T or B lymphocytes to produce this lymphokine. Both T and B lymphocyte populations from guinea-pigs 2-3 weeks after CFA sensitization (early-stage T and B cells) failed to produce significant amounts of BF in response to PPD, whereas both the populations from 10 to 14 weeks after sensitization (late-stage T and B cells) acquired the ability to produce BF by themselves. It is noteworthy that readded autologous Mphi not only functioned well to augment BF production by the late-stage T and B cells but also completely made up for the inability of the early-stage T and B cells to produce BF. From these data it is reasonable to conclude that in the guinea-pig sensitized T and B cells can both release BF on exposure to PPD and that Mphi either reconstitute or augment the BF synthetic response of T and B cells.
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D001402:B-Lymphocytes / Q000276:immunology*; D005260:Female; D006168:Guinea Pigs; D008198:Lymph Nodes / Q000166:cytology; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000096:biosynthesis*; D008264:Macrophages / Q000276:immunology*; D008937:Mitomycins / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology*; D014373:Tuberculin / Q000494:pharmacology*
D008222:Lymphokines; D008937:Mitomycins; D014373:Tuberculin