The identification of urogastrone in serum, saliva, and gastric juice.
Urogastrone, a peptide isolated from human urine, is known to cause inhibition of gastric acid secretion and proliferation of fibroblasts in culture; furthermore immunofluorescent localization techniques show it to be present in submandibular and Brunner's glands. Serum, saliva, and gastric juice samples have now been fractionated upon Sephadex G-200 and G-50 and the immunoreactive urogastrone located using a specific radioimmunoassay. Biologic activity was shown by mitogenic studies with 3T6 fibroblasts. In serum, the major immunoreactive component was ca. 1-2 X 10(5) daltons, but trypsin treatment then gave a smaller biologically active species in the same position as pure urogastrone on Sephadex G-50. Both saliva and gastric juice showed major components at the position defined by urogastrone, and these also stimulated the uptake of [H]thymidine into the fibroblasts. It is concluded that a urogastrone-like molecule can be released enzymically from a high molecular weight serum precursor and that the small biologically active peptide is also a normal component of saliva and gastric juice.
D001345:Autoradiography; D001681:Biological Assay / Q000379:methods*; D002850:Chromatography, Gel; D004815:Epidermal Growth Factor / Q000032:analysis* / Q000097:blood; D005347:Fibroblasts / Q000378:metabolism; D005750:Gastric Juice / Q000032:analysis*; D005768:Gastrointestinal Hormones / Q000032:analysis*; D006801:Humans; D011863:Radioimmunoassay / Q000379:methods*; D012463:Saliva / Q000032:analysis*; D013936:Thymidine / Q000378:metabolism; D014357:Trypsin; D014360:Trypsin Inhibitor, Kunitz Soybean
D005768:Gastrointestinal Hormones; D004815:Epidermal Growth Factor; D014360:Trypsin Inhibitor, Kunitz Soybean; D014357:Trypsin; D013936:Thymidine