Development of B cell subsets: effect of priming on in vitro TNP-LPS responsiveness.
Mice were injected with DNP-derivatives of thymus dependent (DNP-OA) and thymus-independent (DNP-Ficoll and TNP-LPS) antigens, and the response to TNP-LPS in vitro was studied after several priming periods. DNP-OA priming decreases the amount of cells responding to TNP-LPS in vitro. In the case of DNP-Ficoll and TNP-LPS-primed cells, there is an initial burst of responsiveness to TNP-LPS, which progressively decreases until an abolishment of TNP-LPS responsiveness is found at day 40 after immunization. The sensitivity to TNP-LPS reappears as new precursor cells differentiate into mature cells. We suggest that B cells progressively gain the capacity to respond to thymus-independent and thymus-dependent antigens (B1-B2 differentiation) and that challenge with a particle antigen increases the ratio of maturation throughout the pathway.
D000818:Animals; D000921:Antibody-Producing Cells / Q000276:immunology; D000941:Antigens; D001402:B-Lymphocytes / Q000276:immunology*; D003433:Crosses, Genetic; D004136:Dinitrobenzenes / Q000276:immunology; D005260:Female; D005362:Ficoll / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology*; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008937:Mitomycins / Q000494:pharmacology; D009578:Nitrobenzenes / Q000276:immunology*; D010047:Ovalbumin / Q000276:immunology; D013154:Spleen / Q000276:immunology; D014301:Trinitrobenzenes / Q000276:immunology*
D000941:Antigens; D004136:Dinitrobenzenes; D008070:Lipopolysaccharides; D008937:Mitomycins; D009578:Nitrobenzenes; D014301:Trinitrobenzenes; D005362:Ficoll; D010047:Ovalbumin