Factors influencing the inactivation of aminoglycosides by beta-lactams.
Aminoglycosides and beta-lactam antibiotics were tested alone and in combination, via bioassay, in the presence of pH buffers at 6.5, 7.5 and 8.5; human serum; MgCl2 and CaCl2; clavulanic acid and beta-lactamase. Results show: inactivation of gentamicin, tobramycin, sisomicin and netilmicin is influenced by pH; clavulanic acid can inactivate gentamicin and tobramycin; and inactivation is influenced by salt concentration and constituents.
D000617:Aminoglycosides / Q000037:antagonists & inhibitors; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D004347:Drug Interactions; D001618:beta-Lactamases / Q000378:metabolism; D047090:beta-Lactams / Q000494:pharmacology
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D047090:beta-Lactams; D001618:beta-Lactamases