Metabolism of arginine-specific messenger ribonucleic acid in Escherichia coli K-12.
Ribonucleic acid-deoxyribonucleic acid (RNA-DNA) hybridization was employed for the determination of the level of messenger RNA (mRNA) transcribed from seven of the nine genes of the arginine regulon of Escherichia coli K-12. The quantity of RNA complexing with each of the separated DNA strands of the argA, argF, argE, and argCBH operons carried on specialized transducing phages was measured. The derepressed:repressed ratio of mRNA formed in vivo was found to vary between about 3 and 4 when measured by hybridization to DNA isolated from specialized transducing phages carrying the argA, argE, argCBH, argF, and argI operons.
D001120:Arginine / Q000096:biosynthesis*; D003090:Coliphages; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D009876:Operon; D012329:RNA, Bacterial / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D014158:Transcription, Genetic; D014161:Transduction, Genetic
D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012333:RNA, Messenger; D001120:Arginine