The use of a novel plate assay in a search for yeast mutants defective in deoxyribonucleases.
Methods by which the intracellular enzymes deoxyribonuclease, ribonuclease and protease can be assayed in whole colonies of Saccharomyces cerevisiae on agar plates are described. A search for mutants deficient in deoxyribonuclease has been carried out. Two types of mutant are described. One apparently fails to produce deoxyribonuclease, ribonuclease or protease on agar plates and the other apparently fails to produce deoxyribonuclease and ribonuclease.
D004796:Clinical Enzyme Tests / Q000379:methods*; D003851:Deoxyribonucleases / Q000378:metabolism*; D009154:Mutation; D010447:Peptide Hydrolases / Q000378:metabolism; D012260:Ribonucleases / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
D003851:Deoxyribonucleases; D012260:Ribonucleases; D010447:Peptide Hydrolases