title: Crossed immunoelectrophoretic analysis of chromatophore membranes from Rhodopseudomonas sphaeroides.
abstract: Triton extracts of intracytoplasmic photosynthetic membranes (chromatophores) purified from Rhodopseudomonas sphaeroides were subjected to crossed immunoelectrophoresis with antiserum raised in rabbits to purified chromatophores. A total of 31 immunoprecipitates was visualized; 2 of the immunoprecipitates were identified as reduced nicotinamide adenine dinucleotide (EC 1.6.99.3) and L-lactate dehydrogenases by enzyme staining techniques. Reaction with a monospecific antiserum identified the photochemical reaction center. Photopigments were associated with a major precipitate in the pattern which was identified on the basis of immunological identity as light-harvesting bacteriochlorophyll a . protein complex. These results provide the basis for a detailed structural and functional analysis of the chromatophore membrane by crossed immunoelectrophoresis.
mesh_terms: D020130:Bacterial Chromatophores / Q000032:analysis*; D001436:Bacteriorhodopsins / Q000032:analysis*; D002338:Carotenoids / Q000032:analysis*; D007123:Immunoelectrophoresis, Two-Dimensional; D008565:Membrane Proteins / Q000032:analysis*; D010788:Photosynthesis; D012242:Rhodobacter sphaeroides / Q000032:analysis*
chemical_list: D008565:Membrane Proteins; D002338:Carotenoids; D001436:Bacteriorhodopsins