title: Role of macrophage-derived factors in generation of cytotoxic and antibody responses.
abstract: The ability of macrophage subpopulations, separated by virtue of differences in size, to liberate soluble factors able to reconstitute antibody and cell-mediated immune responses in macrophage-depleted lymphoid cultures has been investigated. Evidence is presented to show that all macrophages release trypsin- and pepsin-sensitive molecules with molecular weights of 30,000 to 35,000 and 50,000 to 70,000 that are able to reconstitute only syngeneic lymphocyte responses. In contrast, a molecule(s) with molecular weight of 10,000 to 15,000 daltons is released only by large macrophages and can apparently reconstitute both syngeneic and allogeneic lymphocyte responses. This material is also pepsin-sensitive and, to a lesser degree, trypsin-sensitive.
mesh_terms: D000818:Animals; D000917:Antibody Formation; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D003851:Deoxyribonucleases / Q000494:pharmacology; D007111:Immunity, Cellular / Q000187:drug effects; D008214:Lymphocytes / Q000276:immunology; D008264:Macrophages / Q000276:immunology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008970:Molecular Weight; D010434:Pepsin A / Q000494:pharmacology; D014357:Trypsin / Q000494:pharmacology
chemical_list: D003851:Deoxyribonucleases; D014357:Trypsin; D010434:Pepsin A