Effect of maternal vitamin A excess on S-100 in neonatal rat cerebellum: a preliminary study.
Pregnant rats were administered a teratogenic dose of vitamin A excess on Days 17 and 18 of gestation and intubation controls received the vehicle alone. Pups were sacrificed at birth or on postnatal Days 5 or 10; the cerebellum was removed, frozen, and fixed in preparation for immunohistochemical localization of the brain specific protein, S-100. Vitamin A produced a transient delay in the appearance of S-100 in the external granular layer which was no longer evident on Days 5 and 10. These findings corroborate autoradiographic evidence that vitamin A temporarily interferes with neurogenesis. However, the early damage results in permanent behavioral deficits in adulthood despite apparent cytochemical repair.
D000818:Animals; D002531:Cerebellum / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D005260:Female; D006652:Histological Techniques; D008431:Maternal-Fetal Exchange; D009419:Nerve Tissue Proteins / Q000096:biosynthesis*; D011247:Pregnancy; D051381:Rats; D009418:S100 Proteins / Q000096:biosynthesis*; D014801:Vitamin A / Q000008:administration & dosage / Q000633:toxicity*
D009419:Nerve Tissue Proteins; D009418:S100 Proteins; D014801:Vitamin A