title: Influence of cationic triphenylmethane dyes upon DNA polymerization and product hydrolysis by Escherichia coli polymerase I.
abstract: The cationic triphenylmethane dyes crystal violet, methyl green, and malachite green inhibited DNA synthesis catalyzed by Escherichia coli B polymerase I (polymerase I). Lower concentrations of the dyes inhibited DNA replication as a direct function of the proportion of AT to GC in the DNA of Clostridium perfringens, Escherichia coli, and Micrococcus lysodeikticus. When the intercalant proflavin was employed, the GC-rich micrococcal DNA was most severely inhibited. In addition, both the triphenylmethanes and proflavin inhibited product hydrolysis catalyzed by polymerase I.
mesh_terms: D004396:Coloring Agents / Q000494:pharmacology*; D004256:DNA Polymerase I / Q000378:metabolism*; D004261:DNA Replication / Q000187:drug effects; D004269:DNA, Bacterial / Q000378:metabolism*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology*; D007700:Kinetics; D011370:Proflavine / Q000494:pharmacology; D014320:Triphenylmethyl Compounds / Q000494:pharmacology*
chemical_list: D004396:Coloring Agents; D004269:DNA, Bacterial; D014320:Triphenylmethyl Compounds; D011370:Proflavine; D004256:DNA Polymerase I; D004259:DNA-Directed DNA Polymerase