A rapid technic for quantitating wound bacterial count.
An improved technic has been developed to predict rapidly the critical number of bacteria in tissues. This technic gave reliable and accurate measurements for wounds containing more than 2 X 10(5) organisms per gram of tissue. It is fortuitous that 10(6) bacteria is the critical number of bacteria that will induced clinical infection in experimental animals and humans. The rapid slide technic is now being utilized routinely in our medical center to determine graft bed receptiveness, predict the safety of wound closure, and monitor the course of burns.
D000818:Animals; D001431:Bacteriological Techniques; D004926:Escherichia coli / Q000254:growth & development; D004927:Escherichia coli Infections / Q000382:microbiology; D006168:Guinea Pigs; D006801:Humans; D008297:Male; D013203:Staphylococcal Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000254:growth & development; D014946:Wound Infection / Q000382:microbiology*