Determination of IgG antibodies produced by the kinetoplast fraction antigens of Trypanosoma rhodesiense, T. vivax and T. brucei by enzyme linked immunosorbent assay.
Antibodies to trypanosome homegenate and subcellular particles of different trypanosome species, with emphasis on the kinetoplast fraction, were measured by the ELISA method. The antibodies formed to these homogenates and subcellular particles could be titrated; the kinetoplast fraction, particularly, not only gave titration against a heterologous strain, but also showed activity against this strain after two to three antigenic variations.
D000818:Animals; D007074:Immunoglobulin G / Q000032:analysis*; D007163:Immunosorbent Techniques; D011817:Rabbits; D051381:Rats; D013347:Subcellular Fractions / Q000276:immunology; D014345:Trypanosoma / Q000276:immunology*; D014346:Trypanosoma brucei brucei / Q000276:immunology
D007074:Immunoglobulin G