Colloidal gold, a useful marker for transmission and scanning electron microscopy.
Electron dense markers of a size suitable for transmission electron microscopy and scanning electron microscopy have been prepared with gold granules labeled with a monolayer of specific macromolecules. The optimum conditions for preparing the markers have been ascertained. The method is simple, rapid and seems to be general since gold granules have been labeled with polysaccharides and proteins. As homogeneous populations of gold granules having different sizes can be prepared, the method is also suitable for double marking experiments. The gold technique is illustrated by the localization of polysaccharides and glycoproteins on yeast cell walls and erythrocyte membranes by transmission electron microscopy and on yeast cells and intact erythrocytes by scanning electron microscopy. Good spatial resolution of the marker was achieved in all cases. The method is also suitable for marking thin sections. Spectrophotometric measurements were used to determine the number of gold granules adsorbed per cell.
D002175:Candida / Q000032:analysis; D002374:Catalase / Q000032:analysis; D002473:Cell Wall / Q000032:analysis / Q000648:ultrastructure; D003102:Colloids; D004912:Erythrocytes / Q000648:ultrastructure; D006046:Gold; D006801:Humans; D008351:Mannans / Q000032:analysis / Q000276:immunology; D008854:Microscopy, Electron / Q000379:methods*; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000032:analysis / Q000648:ultrastructure
D003102:Colloids; D008351:Mannans; D006046:Gold; D002374:Catalase