5'-nucleotidase of B and T lymphocytes isolated from human peripheral blood.
The ecto-5'-nucleotidase activities of highly purified T and B lymphocytes from human peripheral blood have been investigated using biochemical and histochemical techniques. The enzyme activity of the purified B cells was about 3.5 times that of the T cells. Using a histochemical assay, 21--55% of the B cells stained positively for 5'-nucleotidase, but only 2--22% of the T cells were positive. These results are discussed in relation to the low 5'-nucleotidase activities found on peripheral blood lymphocytes from patients with chronic lymphatic leukaemia and some patients with primary hypogammaglobulinaemia.
D000361:Agammaglobulinemia / Q000097:blood / Q000201:enzymology; D001402:B-Lymphocytes / Q000201:enzymology*; D002478:Cells, Cultured; D006651:Histocytochemistry; D006801:Humans; D007945:Leukemia, Lymphoid / Q000097:blood / Q000201:enzymology; D009708:Nucleotidases / Q000097:blood*; D012016:Reference Values; D013601:T-Lymphocytes / Q000201:enzymology*
D009708:Nucleotidases