Improved methods of producing precipitating aspergillus antigens.
Improved methods for producing Aspergillus fumigatus antigens capable of giving sensitive and reproducible results by counterimmunoelectrophoresis (CIEP) are described. Among the factors investigated were the source of casein hydrolysate used for N source, method of incubating the CIEP slide following a run, effect of freeze pressing and lyophilization, age of culture and ratio of C to N source in the medium.
D000946:Antigens, Fungal / Q000302:isolation & purification*; D001232:Aspergillus fumigatus / Q000276:immunology* / Q000378:metabolism; D002244:Carbon / Q000378:metabolism; D002364:Caseins; D003378:Counterimmunoelectrophoresis; D005615:Freezing; D008722:Methods; D009584:Nitrogen / Q000378:metabolism; D011233:Precipitin Tests; D013997:Time Factors
D000946:Antigens, Fungal; D002364:Caseins; D002244:Carbon; D009584:Nitrogen