[Lipid composition of Escherichia coli cells sensitive and resistant to tetracycline].
The following variants of E. coli resistant to tetracycline have been obtained from a strain of E. coli W-677 susceptible to 2.5 mcg/ml of the antibiotic: Ri, a recombinant resulting from crossing E. coli W-677 (recipient) and E. coli CSH-222 (donor), resistant to 200 mcg/ml of tetracycline; R2, a strain isolated upon numeros passaging E. coli W-677 on media containing the antibiotic, resistant to 25 mcg/ml of tetracycline. The ability of the resistant variants to bind the antibiotic decreased, as was found in experiments with 14C-tetracycline. Preincubation with low concentrations of the antibiotic resulted in an additional decrease of the sorption by the resistant variants, and in a decrease by 15--18 per cent in the case of the susceptible culture. The R2 variant differed in lipid composition from the parent, susceptible culture: it contained less total lipids and unsaturated fatty acids, and more branched fatty acids. The R1 variant hardly differed in lipid composition from the susceptible culture.
D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000032:analysis / Q000187:drug effects*; D005227:Fatty Acids / Q000032:analysis; D008055:Lipids / Q000032:analysis*; D010743:Phospholipids / Q000032:analysis; D011995:Recombination, Genetic; D013752:Tetracycline / Q000494:pharmacology*
D005227:Fatty Acids; D008055:Lipids; D010743:Phospholipids; D013752:Tetracycline
Sostav lipidov v kletkakh Escherichia coli, chuvstvitel'nykh i ustoĭchivykh k tetratsiklinu.