Factor VIII procoagulant activity, factor VIII related antigen and von Willebrand factor in newborn cord blood.
The mean level of factor VIII procoagulant acitivity (VIII:C) and factor VIII related antigen (VIIIR:AG) was normal in 100 newborn cord plasmas, whereas that of von Willebrand factor (VIIIR:WF) activity was slightly lower than normal. On crossed immunoelectrophoresis, 20 of 50 newborn infants had an increased anodal mobility of VIIIR:AG. When the cord plasma showing an abnormal electrophoretic pattern was mixed with normal plasma, two precipitation peaks with a broad base were found. Similar mixing experiments with the abnormal cord plasma and plasma from a patient with atypical von Willebrand's disease did not normalize the electrophoretic mobility of VIIIR:AG. Gel filtration of the cord plasma with an abnormal electrophoretic pattern of VIII:AG, showed that the three activities were all detected at the position corresponding to a molecular weight of about 800 000. The results suggest the presence of qualitative abnormalities of the factor VIII molecule in half of full-term newborn cord plasma.
D000328:Adult; D000941:Antigens / Q000032:analysis*; D001779:Blood Coagulation Factors / Q000032:analysis*; D002850:Chromatography, Gel; D005169:Factor VIII / Q000032:analysis / Q000276:immunology*; D005260:Female; D005312:Fetal Blood / Q000276:immunology*; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D007231:Infant, Newborn; D008970:Molecular Weight; D011247:Pregnancy; D011498:Protein Precursors / Q000097:blood*; D014841:von Willebrand Factor / Q000032:analysis*
D000941:Antigens; D001779:Blood Coagulation Factors; D011498:Protein Precursors; D014841:von Willebrand Factor; D005169:Factor VIII