[The effect of hydrocortisone and 6-methylprednisolone on the cytoplasmic membrane of rat thymocytes in an vitro system].
Hydrocortisone and 6-methylprednisolone increase the binding constant of anilinenaphthalene-sulphonate  (ANS) with the cytoplasmatic thymocytes membrane following a 2--3 hour long incubation in vitro. Over the same period these compounds stimulate the liberation of potassium ions  from the thymocytes. A supravital staining of the thymocytes with Trypan blue did not change during the whole time of the hormones action. The effect of the hydrocortisone and 6-methylprednisolone action on the ANS binding constant and potassium release depende upon the duration of incubation and concentration of the steriod in the medium. Hydrocortisone produces a greater influence than does 6-methylprednisolone.
D000817:Anilino Naphthalenesulfonates / Q000378:metabolism; D000818:Animals; D003593:Cytoplasm / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D006854:Hydrocortisone / Q000494:pharmacology*; D066298:In Vitro Techniques; D008566:Membranes / Q000187:drug effects / Q000378:metabolism; D008775:Methylprednisolone / Q000494:pharmacology*; D011188:Potassium / Q000378:metabolism; D051381:Rats; D013601:T-Lymphocytes / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D013997:Time Factors
D000817:Anilino Naphthalenesulfonates; D011188:Potassium; D006854:Hydrocortisone; D008775:Methylprednisolone
Vliianie gidrokortizona i 6-metilprednizolona na tsitoplazmaticheskuiu membrany timotsitov krys v opytakh in vitro.