[Methods for the detection of antibodies to native (double stranded) DNA (author's transl)].
Three different methods adequate for routine work, i.e. hemagglutination, counterimmunoelectrophoresis and radioimmunoassay, were used in the past in our laboratory to detect antibodies to native (double stranded) DNA. There was a high incidence of false positive or false negative results by counterimmunoelectrophoresis. Hemagglutination reaction was less sensitive in detecting anti-nDNA than was radioimmunoassay. Natural DNA preparations and a synthetic polynucleotide (3H-dAT) were used as antigenic substrates in radioimmunoassay providing well correlating binding values.
D000906:Antibodies / Q000032:analysis*; D000974:Antibodies, Antinuclear / Q000097:blood*; D003378:Counterimmunoelectrophoresis; D004247:DNA / Q000276:immunology; D005188:False Negative Reactions; D005189:False Positive Reactions; D006386:Hemagglutination Tests; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000097:blood; D011863:Radioimmunoassay / Q000379:methods; D012216:Rheumatic Diseases / Q000097:blood
D000906:Antibodies; D000974:Antibodies, Antinuclear; D004247:DNA
Methoden zur Bestimmung von Antikörpern gegen native (doppelstrangige) DNS.