Methods for determining the proliferation kinetics of cells by means of 5-bromodeoxyuridine.
After treatment of cells with 5-bromodeoxyuridine (BUdR), the percentage of completely BUdR-labelled interphase nuclei is greater the longer the BUdR treatment. The labelling effect is visible after staining with the fluorochrome 33258 Hoechst and with Giemsa. Various formulae and a nomogram are presented by means of which the percentage of cells in S period, duration of the S period and the whole cell cycle can be determined by examination of a single preparation or by comparison of several preparations. The methods are tested using cell cultures of Microtus agrestis and compared with autoradiographic methods after labeling with 3H-thymidine.
D000818:Animals; D003411:Arvicolinae; D001345:Autoradiography; D001973:Bromodeoxyuridine / Q000378:metabolism; D002455:Cell Division; D002467:Cell Nucleus / Q000378:metabolism; D046508:Culture Techniques; D003584:Cytological Techniques; D004247:DNA / Q000096:biosynthesis; D005069:Evaluation Studies as Topic; D007700:Kinetics; D008297:Male; D008954:Models, Biological; D013936:Thymidine / Q000378:metabolism
D004247:DNA; D001973:Bromodeoxyuridine; D013936:Thymidine