Deoxyribonucleic acid strand breaks during freeze-drying and their repair in Escherichia coli.
Freeze-drying of Escherichia coli cells caused strand breaks of deoxyribonucleic acid (DNA) in both radiation-sensitive and -resistant strains. However, in the radiation-resistant strain E. coli B/r the damaged DNA was repaired after rehydration, whereas in the radiation-sensitive strain E. coli Bs-1 the damaged DNA was not repaired and the DNA was degraded. Repeated freeze-drying did not break the damaged DNA into smaller pieces.
D004260:DNA Repair / Q000528:radiation effects; D004269:DNA, Bacterial / Q000378:metabolism* / Q000528:radiation effects; D004926:Escherichia coli / Q000378:metabolism* / Q000528:radiation effects; D005612:Freeze Drying; D014466:Ultraviolet Rays
D004269:DNA, Bacterial