Expression of alloantigens LY-5 and LY-6 on cytotoxic effector cells.
With anti-Ly antisera and complement it has been possible to demonstrate that cytotoxic effector cells generated in vitro against allogeneic cells carry the alloantigen Ly-5 and Ly-6. The studies show that antisera directed against the Ly-6 antigens, together with complement, eliminate essentially all of the killer cells, of the appropriate strain, while killing only 50 to 60% of Thy-1+ cells. Anti-Ly-5 antsera and complement lysed 55 to 65% of Thy-1+ cells and killed a significant portion, but not all, of the cytotoxic cells. The later finding was investigated in more detail since it suggested a degree of heterogeneity within the killer cell subpopulation. However, the data did not support that conclusion, at least for allogeneic killer cells.
D000818:Animals; D000961:Antilymphocyte Serum / Q000494:pharmacology; D002469:Cell Separation; D003601:Cytotoxicity Tests, Immunologic; D007111:Immunity, Cellular; D007163:Immunosorbent Techniques; D066298:In Vitro Techniques; D007519:Isoantigens; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D010641:Phenotype; D013045:Species Specificity; D013601:T-Lymphocytes / Q000276:immunology
D000961:Antilymphocyte Serum; D007519:Isoantigens