title: Thermodynamic studies of the reversible association of Escherichia coli ribosomal subunits.
abstract: The kinetics of association of Escherichia coli 30S and 50S ribosomal subunits have been carried out as a function of temperature after a magnesium jump from 1.5 to 3 mM. Turbidimetric recordings combined with a stopped-flow apparatus were used to follow the kinetics. The data show that the rates of formation and dissociation of the 70S particles at 3 mM Mg2+ and +25 degrees C were, respectively: k2 = 10(5) M-1 s-1, k1 = 4,5 X 10(-3) s-1; lowering the temperature decreases the rate constants with activation energies equal to E2 = 7.5 kcal/mol, E1 = 26.5 kcal/mol and enhances the association equilibrium towards the 70S species with an enthalpy change (delta H degrees assoc = -19.9 kcal/mol) dominant over the entropy change (delta S degrees assoc = -33 cal/(deg mol)). These thermodynamic parameters were compared to those obtained from studies on the interactions of codon-anticodon in yeast phenylalanine transfer RNA as well as of ribooligonucleotides. The kinetic and thermodynamic data are shown to be consistent with 16S-23S RNA interaction.
mesh_terms: D001665:Binding Sites; D004926:Escherichia coli / Q000648:ultrastructure*; D007700:Kinetics; D008027:Light; D008274:Magnesium; D009391:Nephelometry and Turbidimetry; D012270:Ribosomes / Q000648:ultrastructure*; D012542:Scattering, Radiation; D013816:Thermodynamics
chemical_list: D008274:Magnesium