Leukocyte migration inhibition factor in atopic dermatitis: induction by concanavalin A in vitro.
Assays for the production of leukocyte migration inhibition factor (LMIF) activity by cultured lymphocytes in the presence of the T-cell mitogen concanavalin A (ConA) have been shown to be highly sensitive for detecting decreases in cell-mediated immunity in patients with malignant diseases and primary and secondary immunodeficiency diseases. We have applied the leukocyte migration inhibition test in agarose using supernatants from ConA-stimulated lymphocyte cultures (indirect leukocyte migration inhibition test in agarose for studying patients with atopic dermatitis. Only 5 of 14 lymphocyte cultures from atopic dermatitis patients produced LMIF when stimulated with ConA, as compared with 34 of 34 controls. This difference is highly significant.
D000293:Adolescent; D000328:Adult; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D003208:Concanavalin A; D003876:Dermatitis, Atopic / Q000276:immunology*; D006801:Humans; D007111:Immunity, Cellular; D008222:Lymphokines / Q000096:biosynthesis; D013601:T-Lymphocytes / Q000276:immunology*
D008222:Lymphokines; D003208:Concanavalin A