Ribosomal abnormality in recA mutants of Escherichia coli.
The tif-1 mutation has been shown to affect protein synthesis in vitro by increasing translational ambiguity (Ephrati-Elizur, Luther-Davies and Hayes, 1976). It is demonstrated here that some recA mutations confer similar abnormality. By comparing suitable combinations of ribosomes and soluble proteins from recA+ and recA cells the defect is shown to be associated with ribosomes. The recA mutation, which suppresses most phenotype characteristics of the tif-1 mutation (Castellazzi, George and Buttin, 1972(b)) does not suppress the ribosomal abnormality. Since the closely linked tif-1 and recA mutations lead to the expression of a common property they may be in the same gene.
D001426:Bacterial Proteins / Q000096:biosynthesis; D004926:Escherichia coli / Q000378:metabolism*; D006358:Hot Temperature; D009154:Mutation; D010641:Phenotype; D012329:RNA, Bacterial / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D011995:Recombination, Genetic; D012270:Ribosomes / Q000378:metabolism*
D001426:Bacterial Proteins; D012329:RNA, Bacterial; D012333:RNA, Messenger