Macrophage-lymphocyte association in in vitro mouse spleen cultures; the formation of B-cell colonies.
Colonies of predominantly B lymphocytes have been grown in liquid culture, without agar or specific mitogen treatment. An essential component of the colonies is a macrophage-like cell. Colonies appear to result from continued growth of B lymphocytes following a primary macrophage-lymphocyte association which frequently occurs under a variety of culture conditions. Factors promoting colony growth are foetal calf serum and 2 mercapto-ethanol, the latter acting in part through reduction of a non-dialysable sulphur-containing component of serum.
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology*; D001769:Blood; D002417:Cattle; D002478:Cells, Cultured; D002999:Clone Cells / Q000166:cytology; D003470:Culture Media; D008264:Macrophages / Q000166:cytology*; D008623:Mercaptoethanol; D051379:Mice; D008807:Mice, Inbred BALB C; D008855:Microscopy, Electron, Scanning; D013154:Spleen / Q000166:cytology*
D003470:Culture Media; D008623:Mercaptoethanol