Polysaccharides immobilized in polyacrylamide gel as high-capacity adsorbents for Bandeiraea simplicifolia lectin and concanavalin A.
A high-capacity adsorbent for lectins was easily prepared by immobilization of polysaccharides in a polyacrylamide gel. The properties of granulated guaran gel, used to purify by affinity chromatography the alpha-D-galactose-binding lectin from the seeds of Bandeiraea simplicifolia, were studied, as well as the binding of Concanavalin A (tetrameric) and succinylated Concanavalin A (dimeric) to a yeast mannan gel.
D000178:Acrylamides; D002846:Chromatography, Affinity / Q000379:methods; D003208:Concanavalin A / Q000302:isolation & purification*; D007700:Kinetics; D037102:Lectins / Q000302:isolation & purification*; D008351:Mannans; D037121:Plant Lectins; D011134:Polysaccharides; D012441:Saccharomyces cerevisiae; D012639:Seeds
D000178:Acrylamides; D037102:Lectins; D008351:Mannans; D037121:Plant Lectins; D011134:Polysaccharides; D003208:Concanavalin A