Antigenic modulation of Friend virus erythroleukemic cells in vitro by serum from mice with dormant erythroleukemia.
Friend leukemia virus (FLV) erythroleukemic cells cultured in medium containing FLV-immune serum from dormant FLV-infected mice undergo modulation of FLV cell surface antigens. Modulation was determined by an increased resistance to FLV antibody-mediated complement-dependent lysis and was associated temporally with the capping of FLV-immune complexes at the cell surface. Modulated cells regained their susceptibility to FLV antibody-mediated complement-dependent lysis when transferred to medium containing normal mouse serum. After 48 h of culture in FLV-immune serum, 26% of the FLV erythroleukemic cells were devoid of FLV cell surface antigens as demonstrated by immunofluoresence. Antigenic modulation occurred to a greater extent in cells maintained in logarithmic growth than in cells in GO or resting phase. FLV-antigenic modulation is discussed as a possible mechanism by which antibody induces and maintains FLV-transformed cells in a dormant state.
D000818:Animals; D000936:Antigen-Antibody Complex; D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D002999:Clone Cells; D003601:Cytotoxicity Tests, Immunologic; D005260:Female; D005455:Fluorescent Antibody Technique; D005622:Friend murine leukemia virus / Q000276:immunology*; D007106:Immune Sera / Q000494:pharmacology*; D007074:Immunoglobulin G / Q000032:analysis; D066298:In Vitro Techniques; D007700:Kinetics; D004915:Leukemia, Erythroblastic, Acute / Q000276:immunology*; D051379:Mice; D008811:Mice, Inbred DBA / Q000276:immunology; D014412:Tumor Virus Infections / Q000276:immunology*
D000936:Antigen-Antibody Complex; D000956:Antigens, Viral; D007106:Immune Sera; D007074:Immunoglobulin G