Effects of colchicine and vinblastine on in vitro gastric secretion.
The effects of colchicine and vinblastine on in vitro bullfrog gastric mucosal preparations were studied with respect to H+ and pepsinogen secretion. In the concentration range of 1--50 mM, an initial but transient colchicine-mediated stimulation of H+ secretion is followed by a dose-dependent inhibition. The transient stimulation of H+ secretion can be confirmed in resting preparations in the absence of added secretagogues. In the same concentration range, colchicine inhibits pepsinogen secretion to a greater degree than H+ secretion. Vinblastine (10(-5)--5 X 10(-4) M) was more effective than colchicine in inhibiting both H+ and pepsinogen secretion. The kinetics of inhibition of secretion by both colchicine and vinblastine were slow. Cytochalasin B had no effect on either secretion.
D000818:Animals; D001001:Anura; D003078:Colchicine / Q000494:pharmacology*; D003571:Cytochalasin B / Q000494:pharmacology; D003864:Depression, Chemical; D004305:Dose-Response Relationship, Drug; D005750:Gastric Juice / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism*; D006632:Histamine / Q000494:pharmacology; D066298:In Vitro Techniques; D007700:Kinetics; D008297:Male; D010435:Pepsinogens / Q000378:metabolism; D011892:Rana catesbeiana / Q000378:metabolism; D014747:Vinblastine / Q000494:pharmacology*
D010435:Pepsinogens; D003571:Cytochalasin B; D014747:Vinblastine; D006632:Histamine; D003078:Colchicine