Association kinetics with coupled diffusion. An extension to coiled-chain macromolecules applied to the lac repressor-operator system.
The association of a molecule onto a specified binding site on a large chain-like macromolecule is described in the "sliding" model, where the molecule is allowed to move along the chain in a one-dimensional diffusion which is coupled to the three-dimensional diffusion in solution. The present work extends a previous one by treating the chains more generally as coiled instead of straight. The model is applied to the lac repressor-operator association. A general expression for the rate of unspecific attachment to a chain-like macromolecule is also derived.
D004269:DNA, Bacterial / Q000378:metabolism; D004058:Diffusion; D004926:Escherichia coli / Q000378:metabolism*; D007700:Kinetics; D007785:Lactose / Q000378:metabolism*; D046911:Macromolecular Substances; D008433:Mathematics; D008954:Models, Biological
D004269:DNA, Bacterial; D046911:Macromolecular Substances; D007785:Lactose