Monoclonal antibody directed to a B-cell antigen present in rats, mice, and humans.
Spleen cells from a LEW.AVN rat immunized with cells from an MNR rat were fused with mouse myeloma cells to produce hybrid cell lines. One of these hybridomas produced a monoclonal antibody that was cytotoxic for bone marrow-derived (B) but not thymus-derived (T) cells. The antigen defined by this antibody is determined by a gene linked to the major histocompatibility complex (MHC). The antigen is also present on B cells of most mouse strains and is determined by an MHC-linked gene in this species as well. In both rats and mice, the gene determining the antigen maps within the immune response region of the MHC. All human B-cell lines, but not T-cell lines, and B but not T cells of all human donors tested so far are also positive for this antigen. Among human-mouse somatic cell lines that have lost various human chromosomes, this B-cell antigen is present on all lines that are positive for HLA antigen but is absent from all lines that have lost HLA.
D000818:Animals; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D000954:Antigens, Surface / Q000032:analysis*; D001402:B-Lymphocytes / Q000276:immunology*; D002999:Clone Cells / Q000276:immunology; D005796:Genes; D006801:Humans; D006822:Hybrid Cells / Q000276:immunology; D007518:Isoantibodies; D007519:Isoantigens / Q000032:analysis*; D008285:Major Histocompatibility Complex; D051379:Mice; D051381:Rats; D013045:Species Specificity
D000954:Antigens, Surface; D007518:Isoantibodies; D007519:Isoantigens