Activated human T lymphocytes display new surface glycoproteins.
We have analyzed the surface glycoproteins of resting and in vitro activated human T lymphocytes by the galactose oxidase/NaB3H4 and the periodate/NaB3H4 labeling techniques. The labeled glycoproteins were separated by polyacrylamide slab gel electrophoresis and visualized by fluorography. A "new" glycoprotein with an apparent molecular weight of 130,000 (GP130) was strongly labeled on alloantigen-activated T blasts but only weakly or not at all on mitogen-stimulated T blasts and resting T lymphocytes. These results demonstrate that human T cells, as earlier found in the mouse system, express different surface molecules in relation to the particular mode of activation and stage of differentiation.
D001402:B-Lymphocytes / Q000276:immunology; D006023:Glycoproteins / Q000097:blood / Q000276:immunology*; D006801:Humans; D007519:Isoantigens; D008213:Lymphocyte Activation; D008565:Membrane Proteins / Q000097:blood / Q000276:immunology*; D008934:Mitogens; D008970:Molecular Weight; D013601:T-Lymphocytes / Q000276:immunology*
D006023:Glycoproteins; D007519:Isoantigens; D008565:Membrane Proteins; D008934:Mitogens