title: Improved methods for separation and purification by affinity chromatography.
abstract: Defined conditions for simplified Affinity Chromatography suitable for instructional and research purposes, were developed. The standard method of activation of polysaccharide gels using BrCN was modified and improved using a highly buffered reaction solution. A batchwise protocol for the immunoadsorbent isolation of anti-BSA was used (with commonly employed dissociation agents) and comparative data were obtained.
mesh_terms: D000097:Acetonitriles; D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods; D003488:Cyanogen Bromide; D006041:Goats; D007163:Immunosorbent Techniques; D012710:Serum Albumin, Bovine / Q000276:immunology
chemical_list: D000097:Acetonitriles; D000906:Antibodies; D012710:Serum Albumin, Bovine; D003488:Cyanogen Bromide