Pseudocoagulase activity of staphylococci.
A total of 245 strains of staphylococci isolated from various pathological specimens derived from cases of human infections was tested for staphylocoagulase activity. Test systems employing normal citrated rabbit plasma and the same substrate supplemented with inhibitors of thrombin and proteolytic enzymes (but not influencing the staphylocoagulase activity) were used for testing suspensions of bacteria and cell-free culture supernatants. A total of 237 strains clotted normal rabbit plasma; however, addition of Trasylol and heparin resulted in positive results in 222 strains, whereas plasma supplemented with Trasylol and hirudin was coagulated definitely by only 173 strains. It is postulated that proteolytic enzymes of staphylococci interfere with staphylocoagulase-induced clotting and may simulate coagulase-positive activity of staphylococci. To avoid such false results, a test system for detection of staphylocoagulase should include proteolytic enzyme inhibitors. Possible mechanisms of these findings are discussed.
D000818:Animals; D007611:Aprotinin / Q000494:pharmacology; D001777:Blood Coagulation / Q000187:drug effects; D001780:Blood Coagulation Tests; D003030:Coagulase / Q000378:metabolism*; D006629:Hirudins / Q000494:pharmacology; D006801:Humans; D010949:Plasma / Q000502:physiology; D011817:Rabbits; D013203:Staphylococcal Infections / Q000382:microbiology*; D013210:Staphylococcus / Q000201:enzymology*
D003030:Coagulase; D006629:Hirudins; D007611:Aprotinin