Increased survival of CBA pluripotent haemopoietic stem cells in vitro induced by a marrow stromal factor in Sl/Sld mice.
Media conditioned by marrow adherent cells from anaemic Sl/Sld and W/Wv mice increased the 24-h survival of CBA CFUs in vitro compared to fresh medium to about the same extent as marrow-conditioned medium from normal Sl+/Sl+, W+/W+, and CBA mice. Sl/Sld marrow-conditioned medium also increased the percentage of CFUs in DNA synthesis to the same extent as CBA marrow-conditioned medium. These results demonstrate that Sl/Sld mice produce a marrow stromal factor that increases both survival of CFUs and the percentage of CFUs in DNA synthesis in vitro. Therefore, the defective haemopoietic microenvironment of Sl/Sld mice is not due to a deficiency in the production of this factor.
D000818:Animals; D001853:Bone Marrow / Q000032:analysis; D001854:Bone Marrow Cells; D002470:Cell Survival; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D003115:Colony-Stimulating Factors; D003470:Culture Media; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000502:physiology; D051379:Mice; D008808:Mice, Inbred CBA; D008815:Mice, Inbred Strains
D003115:Colony-Stimulating Factors; D003470:Culture Media