Use of a free viral immunofluorescence assay to detect human reovirus-like agent in human stools.
Human reovirus-like agent (HRVLA) is a major cause of gastroenteritis in infants and young children in many parts of the world. Detection of HRVLA in stools is impractical with the techniques currently available. We describe a rapid immunofluorescence assay for detection of HRVLA in stools. Results with this assay agreed well with results obtained from examination of stool specimens by electron microscopy.
D000818:Animals; D000936:Antigen-Antibody Complex; D001667:Binding, Competitive; D002417:Cattle; D005243:Feces / Q000382:microbiology*; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D006801:Humans; D012087:Reoviridae / Q000302:isolation & purification* / Q000648:ultrastructure; D013552:Swine
D000936:Antigen-Antibody Complex