Effects of tetracycline and diazepam on insulin secretion and adenylyl cyclase activity of isolated rat islets of Langerhans.
Tetracycline (2 and 20 micronM) inhibited glucose-stimulated insulin secretion from isolated rat islets of Langerhans, although it failed to alter tolbutamide-stimulated insulin release. Diazepam (10 micronM) potentiated tolbutamide-stimulated insulin release and at a concentration of 1 mM increased glucose-stimulated insulin release. Tetracycline (20 and 200 micronM) or diazepam (10, 100 and 1,000 micronM) inhibited adenylyl cyclase activity of islets homogenate. These results suggest that the effect of tetracycline on insulin secretion might be in part due to inhibition of adenylyl cyclase of the islets. However, the effect of diazepam on insulin secretion is not mediated through the adenylyl cyclase system.
D000067956:Adenylyl Cyclase Inhibitors; D000262:Adenylyl Cyclases / Q000378:metabolism*; D000818:Animals; D003975:Diazepam / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D005947:Glucose / Q000494:pharmacology; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D013752:Tetracycline / Q000494:pharmacology*; D014044:Tolbutamide / Q000494:pharmacology
D000067956:Adenylyl Cyclase Inhibitors; D007328:Insulin; D014044:Tolbutamide; D000262:Adenylyl Cyclases; D013752:Tetracycline; D005947:Glucose; D003975:Diazepam