title: Characterization of the collagen synthesized by cultured human smooth muscle cells from fetal and adult aorta.
abstract: Smooth muscle cells were grown from explants of the tunica media of fetal and adult human aorta. Collagen was isolated after incubation with [14C]glycine and was characterized by ion-exchange chromatography. All cells investigated synthesized two types of collagen: Type I (chain composition [alpha1(I)]2alpha2) and type III (chain composition [alpha1(III)]3). The collagen made by cells from adult donors contained approximately 70% type I and 30% type III collagen. This corresponds to the collagen composition in teh original tissue. No age-relate change in the type I/type III ratio was found with cells from donors between 9 and 67 years of age. On the other hand, the type III portion of the collagen made by fetal cells was markedly less (about 15-20% of total collagen).
mesh_terms: D000328:Adult; D001013:Aorta, Thoracic / Q000378:metabolism* / Q000648:ultrastructure; D002478:Cells, Cultured; D002648:Child; D003094:Collagen / Q000096:biosynthesis; D005260:Female; D005333:Fetus; D005347:Fibroblasts / Q000378:metabolism / Q000648:ultrastructure; D005998:Glycine / Q000378:metabolism; D006801:Humans; D008297:Male; D008858:Microscopy, Phase-Contrast; D008875:Middle Aged; D008970:Molecular Weight
chemical_list: D003094:Collagen; D005998:Glycine