The leucocyte migration inhibition test and subpopulations of peripheral lymphocytes in insulin-dependent diabetics.
The leucocyte migration inhibition test (LMT) was performed by the agarose plate method with thyroid and pancreatic antigens in patients with insulin-dependent or independent diabetes mellitus. The mean migration indices with thyroglobulin, thyroid mitochondria and beef insulin were not significantly different in insulin-dependent diabetics from those in insulin-independent diabetics or normal controls. However, significant inhibition of leucocyte migration was observed in insulin-dependent diabetics when thyroid microsome or pancreatic extract was used as antigen. Although no significant difference was found in the percentages of T and B lymphocytes between insulin-dependent diabetics and insulin-independent diabetics or normal controls, the results of LMT strongly suggest the presence of cellular immunity against the thyroid and pancreas in insulin-dependent juvenile-onset diabetes.
D000328:Adult; D000368:Aged; D000906:Antibodies / Q000032:analysis; D001402:B-Lymphocytes / Q000276:immunology*; D002464:Cell Migration Inhibition; D003920:Diabetes Mellitus / Q000276:immunology*; D006801:Humans; D007328:Insulin / Q000627:therapeutic use; D007958:Leukocyte Count; D008875:Middle Aged; D010179:Pancreas / Q000276:immunology; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000276:immunology*; D013961:Thyroid Gland / Q000276:immunology
D000906:Antibodies; D007328:Insulin