A rapid, semi-automated counting procedure for enumeration of antibody-forming cells in gel and nucleated cells in suspension.
A commercially available bacterial colony counter has been modified to permit rapid, highly accurate, semi-automated enumeration of antibody-producing plaque forming cells in semi-solid support medium as well as enumeration of nucleated cells in suspension on a standard hemacytometer chamber. This apparatus should therefore serve as an enormous time-conserving accessory to most modern laboratories involved in immunological research.
D000818:Animals; D000921:Antibody-Producing Cells; D001322:Autoanalysis; D002452:Cell Count / Q000379:methods; D002467:Cell Nucleus; D004136:Dinitrobenzenes / Q000276:immunology; D006462:Hemolytic Plaque Technique; D007074:Immunoglobulin G; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D010400:Penicillin G / Q000276:immunology; D013154:Spleen / Q000166:cytology*; D013535:Suspensions; D013997:Time Factors
D004136:Dinitrobenzenes; D007074:Immunoglobulin G; D013535:Suspensions; D010400:Penicillin G