Isolation and characterization of the erythrocyte surface membrane of the smooth dogfish, Mustelus canis.
1. The plasma membrane of the dogfish erythrocyte is characterized. 2. Surface membranes were isolated from dogfish red cells. 3. Cells suspended in a hypotonic zinc chloride buffer (0.5 mM ZnCl2, 5 mM Tris-HCl, pH 8.0) tended to enucleate spontaneously. 4. By gentle homogenization relatively high yields of red cell "ghosts" were formed; these were purified using discontinuous gradients of sucrose and of glycerol solutions. Analyses of protein, carbohydrates and lipids were carried out. 5. There did not appear to be marked overall differences in the composition of the surface membrane of the nucleated dogfish erythrocyte compared to those of other species.
D000596:Amino Acids / Q000097:blood; D000818:Animals; D002241:Carbohydrates / Q000097:blood; D002458:Cell Fractionation; D002467:Cell Nucleus; D002499:Centrifugation, Density Gradient; D002712:Chlorides; D004284:Dogfish / Q000097:blood*; D004910:Erythrocyte Membrane / Q000032:analysis / Q000648:ultrastructure*; D005690:Galactose / Q000032:analysis; D007038:Hypotonic Solutions; D008358:Mannose / Q000097:blood; D008563:Membrane Lipids / Q000097:blood; D008565:Membrane Proteins / Q000032:analysis; D019158:N-Acetylneuraminic Acid; D010743:Phospholipids / Q000097:blood; D012754:Sharks / Q000097:blood*; D012794:Sialic Acids / Q000097:blood; D015032:Zinc; D017967:Zinc Compounds
D000596:Amino Acids; D002241:Carbohydrates; D002712:Chlorides; D007038:Hypotonic Solutions; D008563:Membrane Lipids; D008565:Membrane Proteins; D010743:Phospholipids; D012794:Sialic Acids; D017967:Zinc Compounds; C016837:zinc chloride; D019158:N-Acetylneuraminic Acid; D015032:Zinc; D008358:Mannose; D005690:Galactose