title: DNA synthetic ability in cerebella from temperature and handling stressed neonatal rats.
abstract: DNA synthetic ability of neonatal rat cerebellum was studied in two stressful conditions (cooling and excessive handling) which may be inadvertently imposed during the course of certain experiments. A rate study of the ability of animals handled excessively for 10 min to incorporate 14C-thymidine into the DNA tissue fraction (DNAF) from the acid soluble tissue fraction (ASF) of the cerebellum revealed a decreased DNA synthetic ability which lasted for 1 hr. Animals with very low core temperatures incorporated little 14C into the DNAF from the ASF 2 hr after an IP injection of 14C-thymidine. There was a proportional decrease in the ability to synthesize DNA as core temperature decreased.
mesh_terms: D000822:Animal Husbandry; D000818:Animals; D000830:Animals, Laboratory; D000831:Animals, Newborn; D002531:Cerebellum / Q000502:physiology*; D004247:DNA / Q000096:biosynthesis*; D051381:Rats; D012107:Research Design; D013312:Stress, Physiological / Q000235:genetics*; D013696:Temperature
chemical_list: D004247:DNA