title: Deoxyribonucleic acid sequence organization of a yeast plasmid.
abstract: Two-micrometer deoxyribonucleic acid (DNA), a circular plasmid of Saccharomyces cerevisiae, contains two nontandem repeated sequences which are inverted with respect to one another. These repeated sequences together account for 21% of the molecule length. Restriction endonuclease analysis and electron microscopy demonstrated the existence of two forms of 2-mum DNA differing in the orientation of the interstitial segments bounded by the inverted repeated sequences. The two forms of 2-mum DNA could result from an intramolecular reciprocal recombination between inverted repeat elements. A map containing the restriction endonuclease sites and the units of the inverted repeat has been constructed.
mesh_terms: D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004270:DNA, Circular / Q000032:analysis*; D005111:Extrachromosomal Inheritance; D009690:Nucleic Acid Conformation; D010957:Plasmids; D012441:Saccharomyces cerevisiae
chemical_list: D004270:DNA, Circular; D004262:DNA Restriction Enzymes