Isolation of poly-alpha-L-guluronate lyase from Klebsiella aerogenes.
The bacterium Klebsiella aerogenes type 25 produces an extracellular alginolyase which has been partly purified. The enzyme is specific for the alpha-L-guluronosyl linkages in whole alginate and fractions therefrom. The end products of its action on polyguluronic acid blocks are mainly the unsaturated di- and tri-saccharides, with a smaller proportion of the homologous tetrasaccharide. Some general properties of the enzyme are reported.
D000464:Alginates; D002412:Cations; D006603:Hexuronic Acids; D007711:Klebsiella pneumoniae / Q000201:enzymology*; D011133:Polysaccharide-Lyases / Q000302:isolation & purification / Q000378:metabolism; D013379:Substrate Specificity
D000464:Alginates; D002412:Cations; D006603:Hexuronic Acids; D011133:Polysaccharide-Lyases; C022416:poly-alpha-L-guluronate lyase