Isolation of cryptic plasmid deoxyribonucleic acid from Kanagawa-positive strains of Vibrio parahaemolyticus.
Twelve strains of Vibrio parahaemolyticus was examined for plasmid deoxyribonucleic acid (DNA) by dye-buoyant gradient centrifugation. Four Kanagawa-positive strains, all isolated from the same outbreak of gastroenteritis, contained multiple plasmid species of cryptic function. However, three Kanagawa-negative strains and five Kanagawa-positive strains were not found to contain demonstrable plasmid DNA. R-plasmids were successfully transferred from Escherichia coli to V. parahaemolyticus.
D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004926:Escherichia coli; D005111:Extrachromosomal Inheritance; D008854:Microscopy, Electron; D008968:Molecular Conformation; D010641:Phenotype; D010957:Plasmids; D014733:Vibrio
D004269:DNA, Bacterial