Cytochrome P-450 from mitochondria of bovine adrenal cortex: comparison of cholesterol side-chain cleavage P-450 with steroid 11beta-hydroxylation P-450 and immunochemical cross-reactivity between adrenal mitochondrial and liver microsomal cytochromes P-450.
Adrenocortical mitochondrial cytochrome P-450 specific to the cholesterol side-chain cleavage (desmolase) reaction differs from that for the 11beta-hydroxylation reaction of deoxycorticosterone. The former cytochrome appears to be more loosely bound to the inner membrane than the latter. Upon ageing at 0 degrees C or by aerobic treatment with ferrous ions, the desmolase P-450 was more stable than the 11beta-hydroxylase P-450. By utilizing artificial hydroxylating agents such as cumene hydroperoxide, H2O2, and sodium periodate, the hydroxylation reaction of deoxycorticosterone to corticosterone in the absence of NADPH was observed to a comparable extent with the reaction in the presence of adrenodoxin reductase, adrenodoxin and NADPH. However, the hydroxylation reaction of cholesterol to pregnenolone was not supported by these artificial agents. Immunochemical cross-reactivity of bovine adrenal desmolase P-450 with rabbit liver microsomal P-450LM4 was also investigated. We found a weak but significant cross-reactivity between the adrenal mitochondrial P-450 and liver microsomal P-450LM4, indicating to some extent a homology between adrenal and liver cytochromes P-450.
D000302:Adrenal Cortex / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002784:Cholesterol / Q000378:metabolism*; D003429:Cross Reactions; D003577:Cytochrome P-450 Enzyme System / Q000276:immunology / Q000378:metabolism*; D003900:Desoxycorticosterone / Q000378:metabolism*; D004851:Epoxide Hydrolases / Q000037:antagonists & inhibitors; D006900:Hydroxylation; D007501:Iron / Q000494:pharmacology; D008190:Lyases / Q000378:metabolism; D008862:Microsomes, Liver / Q000378:metabolism*; D008928:Mitochondria / Q000378:metabolism; D010545:Peroxides / Q000494:pharmacology; D013252:Steroid 11-beta-Hydroxylase / Q000378:metabolism
D010545:Peroxides; D003900:Desoxycorticosterone; D003577:Cytochrome P-450 Enzyme System; D002784:Cholesterol; D007501:Iron; D013252:Steroid 11-beta-Hydroxylase; D004851:Epoxide Hydrolases; D008190:Lyases