Separation of isoacceptor cysteine transfer ribonucleic acids of bakers' yeasts.
Isoacceptor species of certain amino acid-specific transfer ribonucleic acids (tRNAs) were fractionated by gel permeation chromatography using Sephadex G-100. The separation is attributed to the 20% ethanol-1% NaCl solvent and to the characteristics of Sephadex. Isoacceptor tRNAs specific for cysteine, arginine, phenylalanine, and histidine were recovered from commercial tRNA of yeast by this method. Highly purified cysteine-specific tRNA, obtained by a method which would not be expected to separate isoacceptor molecules when fractionated by this procedure, was shown to contain two cysteine isoacceptor tRNAs.
D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism; D002850:Chromatography, Gel; D003545:Cysteine; D012343:RNA, Transfer / Q000302:isolation & purification*; D012441:Saccharomyces cerevisiae / Q000032:analysis* / Q000201:enzymology
D012343:RNA, Transfer; D000604:Amino Acyl-tRNA Synthetases; D003545:Cysteine