Human monocyte-lymphocyte interaction: a new technique.
A rosette-type assay of the physical interaction between lymphocytes and monocytes after treatment with neuraminidase-galactose oxidase (NGAO) is reported. Monocyte-lymphocyte (ML) rosette formation and subsequent lymphocyte proliferation occurred when either lymphocytes or homologous monocytes were treated with NGAO and cultured together. Maximal ML rosette formation took place at 37 degrees C 4 hr after culture in media containing 10% serum at lymphocyte to monocyte ratios of 10:1 to 20:1. The percentage of rosette formation correlated with the extent of thymidine incorporation when increasing concentrations of NGAO were used. When NGAO-treated monocytes were added to untreated T and non-T lymphocytes, they bound preferentially to T lymphocytes and induced proliferation only in the T subpopulation. These results indicate that the ML rosette assay measures a highly specific monocyte-lymphocyte physical interaction after a mitogenic stimulus which is an early event in lymphocyte activation since it reflects the degree of subsequent lymphocyte proliferation.
D002469:Cell Separation; D002478:Cells, Cultured; D004306:Dose-Response Relationship, Immunologic; D005692:Galactose Oxidase / Q000494:pharmacology; D006801:Humans; D007158:Immunologic Techniques; D007700:Kinetics; D008213:Lymphocyte Activation; D008934:Mitogens / Q000494:pharmacology; D009000:Monocytes / Q000276:immunology*; D009439:Neuraminidase / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology
D008934:Mitogens; D005692:Galactose Oxidase; D009439:Neuraminidase