[Comparative characteristics of cell-free filtrates of Shigella flexneri of different virulence].
It was shown for the first time that the virulent Sh. flexneri strain grown on Luria broth differed from the avirulent one by the yield of readily released surface-located complexes--lipopolysaccharide (determined by rhamnose) and protein into the filtrate. There was no distinct correlation between the strain virulence and the content of rhamnose-determined lipopolysaccharide in the filtrate; growing bacteria in the presence of Ca and Mg ions had no significant influence on the lipopolysaccharide release into the filtrate. Protein release into the cell-free filtrate was thrice that in the virulent shigella strain than in the avirulent one. When bacteria were grown in the presence of Ca ions protein release from the virulent strain increased 1.5-fold and changed but little in the avirulent culture. Cell-free filtrates of the virulent strain produced toxic action on L tissue culture cells; in conjunctival infection of guinea pigs they caused some reduction of the LD50 of the virulent strain and sharply aggravated the course of the infectious process. Heating of the filtrate at 100 degrees C for 15 min decreased their toxic action on L cells. The data obtained indicated that the active biological factor revealed in the virulent strain of Sh. flexneri was protein or its derivative.
D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism; D002118:Calcium / Q000494:pharmacology; D002460:Cell Line; D002474:Cell-Free System; D002478:Cells, Cultured; D003470:Culture Media; D006168:Guinea Pigs; D008070:Lipopolysaccharides / Q000378:metabolism; D046911:Macromolecular Substances; D008274:Magnesium / Q000494:pharmacology; D012763:Shigella flexneri / Q000166:cytology / Q000254:growth & development / Q000472:pathogenicity*; D013347:Subcellular Fractions; D014774:Virulence
D001426:Bacterial Proteins; D003470:Culture Media; D008070:Lipopolysaccharides; D046911:Macromolecular Substances; D008274:Magnesium; D002118:Calcium
Sravnitel'naia kharakteristika beskletochnykh fil'tratov Sh. flexneri raznoĭ virulentnosti.