Idiotype-specific T helper cells are required to induce idiotype-positive B memory cells to secrete antibody.
We have tested the proposition that induction of certain sets of B cell clones to produce antibody requires a signal from T helper cells that recognize idiotypic determinants expressed on Ig receptors of the relevant B cell clones. The approach is based on the analysis of T cell populations required to induce B cells to secrete anti-arsonate antibodies that are marked by a cross-reactive idiotype (CRId). crid+ anti-azophenyl arsonate (Ar) antibodies are produced in A/J strain mice after immunization with Ar keyhole limpet hemocyanin and represent 20--70% of the total anti-Ar antibody response. These studies indicate that antibody secretion by idiotype+ B memory cells requires two signals: one provided by "carrier"-specific Ly-1 cells, and a second delivered by idiotype-specific Ly-1 cells. Both signals are required for optimal induction of idiotype+ B memory clones.
D000818:Animals; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology*; D007130:Immunoglobulin Idiotypes / Q000235:genetics; D007156:Immunologic Memory; D008211:Lymphocyte Cooperation; D051379:Mice; D008805:Mice, Inbred A; D010641:Phenotype; D013154:Spleen / Q000637:transplantation; D013601:T-Lymphocytes / Q000276:immunology*
D007130:Immunoglobulin Idiotypes