Accumulation of ergosta-8,14-dien-3beta-ol by Saccharomyces cerevisiae cultured with an azasterol antimycotic agent.
15-Aza-25-methylene-D-homocholesta-8,14-dien-3beta-ol, an antimycotic agent, at a concentration of 75 ng/ml inhibited ergosterol biosynthetis in Saccharomyces cerevisiae strain 3701B resulting in the accumulation of an unusual sterol. Experimental data presented indicate that this sterol is ergosta-8,14-dien-3beta-ol. The accumulation of the compound is supportive of current models of biosynthetic pathways for sterols in yeast and is consistent with inhibition by the azasterol of the delta14 sterol reductase.
D000935:Antifungal Agents; D001378:Azasteroids / Q000494:pharmacology*; D004875:Ergosterol / Q000031:analogs & derivatives* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000378:metabolism*; D013260:Steroids, Heterocyclic / Q000494:pharmacology*
D000935:Antifungal Agents; D001378:Azasteroids; D013260:Steroids, Heterocyclic; D004875:Ergosterol