Cells reconstituted from cell fragments of two different species multiply and form colonies.
Nuclei (minicells) prepared by cytochalasin-induced enucleation of rat myoblasts (L6) were fused with inactivated Sendai virus to preparations of cytoplasms from mouse fibroblasts (A9). Cell colonies arising from such fusion mixtures were examined 5 days after fusion. In the presence of virus a new type of colony was obtained. The cells in these colonies were derived from reconstituted cells capable of undergoing repeated cell divisions.
D002467:Cell Nucleus / Q000502:physiology / Q000648:ultrastructure; D006822:Hybrid Cells / Q000502:physiology* / Q000648:ultrastructure; D007739:L Cells / Q000502:physiology; D008858:Microscopy, Phase-Contrast