Genetic analysis of Escherichia coli K-12 region I flagellar mutants.
Flagellar mutants in Escherichia coli region I were obtained by selection for resistance to the flagellotropic phage chi. F' elements carrying this region of the E. coli genome were then constructed. Stable merodiploid strains with a flagellar defect on the exogenate and another on the endogenote were prepared. These merodiploids yielded information on the complementation behavior of mutations in this region. Region I was shown to include at least six cistrons, flaV, flaK, flaL, flaM, flaS, and flaT. Mu-induced and deletion fla mutants were also isolated. By using these mutant strains, the transcriptional order was shown to be flaV-flaK-flaL-flaM-flaS-flaT. The definition of region I fla genes and their transcriptional relationships were confirmed by genetic tests with hybrid A phage carrying fla genes in this region.
D002874:Chromosome Mapping; D003090:Coliphages / Q000254:growth & development; D004926:Escherichia coli / Q000648:ultrastructure*; D005407:Flagella / Q000648:ultrastructure*; D005796:Genes; D005816:Genetic Complementation Test; D008242:Lysogeny; D009154:Mutation; D009876:Operon; D014161:Transduction, Genetic