The translocation of Ca2+ across phospholipid bilayers induced by a synthetic neutral Ca2+ -ionophore.
The effect of a neutral synthetic Ca2+ -ligand, which induces selective Ca2+ transport in electrodialysis experiments in bulk membranes, on the Ca2+ permeability of phospholipid bilayers has been investigated. The ligand is able to promote the transport of Ca2+ across synthetic phospholipid bilayers and can therefore be classified as a Ca2+ -ionophore. Its activity is enhanced by the uncoupler carbonyl cyanide p-trifluoromethoxyphenylhydrazone (FCCP). The efficiency of the neutral carrier-mediated Ca2+ transport is rather low as compared with that of the charged Ca2+ -ionophore X537A. The Ca2+ selectivity of the nuetral ionophore is decreased by its incorporation in the low dielectric ambient of the phospholipid bilayer.
D001693:Biological Transport, Active / Q000187:drug effects; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism*; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone / Q000378:metabolism; D004553:Electric Conductivity; D007476:Ionophores / Q000494:pharmacology*; D007832:Lasalocid / Q000494:pharmacology; D008024:Ligands; D008081:Liposomes / Q000378:metabolism; D008567:Membranes, Artificial; D010712:Phosphatidic Acids; D012964:Sodium / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism
D007476:Ionophores; D008024:Ligands; D008081:Liposomes; D008567:Membranes, Artificial; D010712:Phosphatidic Acids; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone; D000001:Calcimycin; D013395:Sucrose; D012964:Sodium; D002118:Calcium; D007832:Lasalocid