Isolation of human T lymphocytes by a negative selection procedure.
A single step method to isolate human T lymphocytes was achieved using a rosette technique to deplete non-T lymphocytes. Erythrocytes coated simultaneously with IgG and complement were used to form rosettes with cells bearing such receptors during a short incubation period and the rosetting cells removed by gradient centrifugation. T lymphocytes were isolated (greater than 96% T cells) without exposing to much experimental manipulations and in good recovery (mean 60%, range 40-75%).
D000818:Animals; D000961:Antilymphocyte Serum / Q000494:pharmacology; D002469:Cell Separation; D010856:Columbidae; D003165:Complement System Proteins; D004912:Erythrocytes / Q000276:immunology; D006168:Guinea Pigs; D006801:Humans; D007141:Immunoglobulin Fc Fragments; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000276:immunology*
D000961:Antilymphocyte Serum; D007141:Immunoglobulin Fc Fragments; D003165:Complement System Proteins