[Method of determination of specific length of histological specimens with the aid of square-net eyeglass].
The author has analytically expressed the relationship between the inaccessible for measurement total length of non-randomly oriented stretched objects and the sum of lengths of their projections on the longitudinal axis of the preparation (or another line). The sum of their projection lengths can be measured by means of a square-net ocular. The specific length of objects on a histological section is proportional to the number of their intersections with the transversal lines of the net and the value of its division and inversly proportional to the cosine of the average meaning of angles between the longitudinal axis of the preparation and the objects under study. Verification of the validity of the formula deduced was carried on in the histological section model prepared by the author. The proposed method ensures determination of the specific length of stretched histological preparations with a no more than 3% error.
D000818:Animals; D001699:Biometry / Q000295:instrumentation / Q000379:methods*; D005069:Evaluation Studies as Topic; D005139:Eyeglasses; D006652:Histological Techniques / Q000295:instrumentation*; D006801:Humans; D008954:Models, Biological
Sposob opredeleniia udel'noĭ dliny ob'ektov na gistologicheskikh preparatakh s pomoshch'iu kvadratno-setchatogo okuliara