Studies on Tyzzer's disease: application of immunofluorescence for detection of Bacillus piliformis and for demonstration and determination of antibodies to it in sera from mice and rabbits.
Bacillus piliformis antigens were demonstrated in smear preparations from infected mouse livers by direct immunofluorescence technique. Mouse serum antibodies against B. piliformis were demonstrated by indirect immunofluorescence technique. The test was employed quantitatively both on sera from experimentally infected mice and on sera from clinically healthy mice from colonies infected with B. piliformis, and could be used for the quantitative demonstration of antibodies in sera from a stock of rabbits with Tyzzer's disease. It was found very useful for the detection of subclinical infection.
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D000917:Antibody Formation; D001407:Bacillus / Q000276:immunology* / Q000302:isolation & purification; D001424:Bacterial Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D007104:Immune Adherence Reaction; D007114:Immunization; D008099:Liver / Q000382:microbiology; D051379:Mice / Q000276:immunology / Q000382:microbiology*; D011817:Rabbits; D012376:Rodent Diseases / Q000276:immunology / Q000382:microbiology
D000907:Antibodies, Bacterial; D005452:Fluoresceins