Effects of polarizing currents and repetitive stimulation on the uptake of amino acids by peripheral nerve.
The effects of polarizing currents and repetitive stimulation on the uptake of representative amino acids have been studied in peripheral nerve. Glutamic acid, lysine, pheylalanine, and GABA were chosen for study as representatives of different carrier-mediated transport mechanisms. Depolarizing currents inhibited uptakes of glutamic acid, lysine, and GABA; maximum inhibitions were 56.1%, 20.0%, and 16.7%, respectively. Pheylalanine uptake was not inhibited by polarizing currents under any experimental conditions. Only glutamic acid uptake was inhibited by hyperpolarizing current, with a maximum inhibition of 43.9%. Stimulation had no significant effect on uptake of lysine, phenylalanine, or GABA but was found to potentiate uptake of glutamic acid by 12%.
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D001001:Anura; D004558:Electric Stimulation; D005971:Glutamates / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D008564:Membrane Potentials; D010649:Phenylalanine / Q000378:metabolism; D011188:Potassium / Q000494:pharmacology; D011892:Rana catesbeiana; D012584:Sciatic Nerve / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D000596:Amino Acids; D005971:Glutamates; D010649:Phenylalanine; D005680:gamma-Aminobutyric Acid; D008239:Lysine; D011188:Potassium