Isolation and fusion studies on protoplasts from pollen tetrads.
Different enzymes were tested for isolation of intact protoplasts from pollen tetrads. About 80% isolation was achieved from pollen tetrads of Cajanus cajan and Zea mays and about 60% from Luffa cylindrica and Lycopersicon esculentum after 4 h of treatment with 5% cellulase. When these mononucleate protoplasts were incubated in presence of 0.05 M CaCl2 in 0.3 M glucose at pH 10.5, 70-80% fusion was achieved. Fusion was rare in sodium nitrate solutions.
D001665:Binding Sites; D002467:Cell Nucleus / Q000648:ultrastructure; D006026:Glycoside Hydrolases; D008858:Microscopy, Phase-Contrast; D018521:Plant Physiological Phenomena; D011523:Protoplasts / Q000502:physiology* / Q000648:ultrastructure
D006026:Glycoside Hydrolases