Electrophoretic analyses of alcohol dehydrogenase, aldehyde dehydrogenase, aldehyde oxidase, sorbitol dehydrogenase and xanthine oxidase from mouse tissues.
1. Cellulose acetate zymograms of alcohol dehydrogenase (ADH), aldehyde dehydrogenase, sorbitol dehydrogenase, aldehyde oxidase, "phenazine" oxidase and xanthine oxidase extracted from tissues of inbred mice were examined. 2. ADH isozymes were differentially distributed in mouse tissues: A2--liver, kidney, adrenals and intestine; B2--all tissues examined; C2--stomach, adrenals, epididymis, ovary, uterus, lung. 3. Two NAD+-specific aldehyde dehydrogenase isozymes were observed in liver and kidney and differentially distributed in other tissues. Alcohol dehydrogenase, aldehyde oxidase, "phenazine" oxidase and xanthine oxidase were also stained when aldehyde dehydrogenase was being examined. 4. Two aldehyde oxidase isozymes exhibited highest activities in liver. 5. "Phenazine oxidase" was widely distributed in mouse tissues whereas xanthine oxidase exhibited highest activity in intestine and liver extracts. 6. Genetic variants for ADH-C2 established its identity with a second form of sorbitol dehydrogenase observed in stomach and other tissues. The major sorbitol dehydrogenase was found in high activity in liver, kidney, pancreas and male reproductive tissues.
D000426:Alcohol Dehydrogenase / Q000032:analysis*; D000444:Aldehyde Dehydrogenase / Q000032:analysis*; D042931:Aldehyde Oxidase; D000445:Aldehyde Oxidoreductases / Q000032:analysis*; D000818:Animals; D004588:Electrophoresis, Cellulose Acetate; D005260:Female; D007064:L-Iditol 2-Dehydrogenase / Q000032:analysis*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009928:Organ Specificity; D013401:Sugar Alcohol Dehydrogenases / Q000032:analysis*; D014969:Xanthine Oxidase / Q000032:analysis*
D013401:Sugar Alcohol Dehydrogenases; D000426:Alcohol Dehydrogenase; D007064:L-Iditol 2-Dehydrogenase; D014969:Xanthine Oxidase; D000445:Aldehyde Oxidoreductases; D000444:Aldehyde Dehydrogenase; D042931:Aldehyde Oxidase