A factor affecting stimulation of aminocylation in plants.
The presence of a factor stimulating the reaction of aminoacylation tRNAs was found in the seeds of lupin, with a molecular weight of 950 as estimated by gel filtration. The influence of this factor on the kinetics of the aminoacylation reactions of lupin, Escherichia coli, Baker's yeast and heterogeneous systems was investigated. This factor inhibits the esterification reaction of aminoacyl-tRNA synthetases from bacteria and yeast. Its influence on the optimum pH activity of isoleucyl-tRNA synthetase from lupin was determined.
D000596:Amino Acids / Q000378:metabolism; D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism*; D004789:Enzyme Activation; D004926:Escherichia coli / Q000201:enzymology; D007532:Isoleucine / Q000378:metabolism; D007533:Isoleucine-tRNA Ligase / Q000378:metabolism; D008970:Molecular Weight; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D012639:Seeds / Q000201:enzymology*; D046249:Transfer RNA Aminoacylation / Q000187:drug effects*
D000596:Amino Acids; D007532:Isoleucine; D000604:Amino Acyl-tRNA Synthetases; D007533:Isoleucine-tRNA Ligase