title: Mechanism of the apparent regulation of Escherichia coli unsaturated fatty acid synthesis by exogenous oleic acid.
abstract: Starvation of strains of Escherichia coli which are glycerol auxotrophs and are also defective in beta oxidation results in the accumulation of large amounts of free fatty acid (Cronan, J. E., Jr., Weisberg, L. W., and Allen, R. G. (1975) J. Biol. Chem. 250, 5835-5840). We now report that addition of exogenous oleic acid to these cultures results in no decrease in the synthesis of the unsaturated acids of the free fatty acid fraction although a 40 to 60% decrease of [14C]acetate incorporation into phospholipid unsaturated acyl moieties occurs under these conditions. This result indicates that the decreased synthesis of phospholipid unsaturated acyl moieties observed by others during oleic acid supplementation can be attributed to competition between exogenous and endogenously synthesized unsaturated fatty acids rather than a curtailment of unsaturated fatty acid synthesis per se.
mesh_terms: D000085:Acetates / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*; D005230:Fatty Acids, Nonesterified / Q000096:biosynthesis; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis*; D005990:Glycerol / Q000378:metabolism / Q000494:pharmacology; D009829:Oleic Acids / Q000494:pharmacology*; D010743:Phospholipids / Q000096:biosynthesis; D011092:Polyethylene Glycols / Q000494:pharmacology
chemical_list: D000085:Acetates; D005230:Fatty Acids, Nonesterified; D005231:Fatty Acids, Unsaturated; D009829:Oleic Acids; D010743:Phospholipids; D011092:Polyethylene Glycols; D005990:Glycerol