Macrophage/T-lymphocyte interaction in the immune response to PPD in humans.
The proliferative response of sensitized human T cells to low concentrations of purified protein derivative of tuberculin (PPD) in vitro has previously been shown to depend on the presence of autologous or HLA-D-compatible macrophages (M phi). In this paper we present evidence that the M phi/T cell interaction requires viable M phi and does not involve a soluble factor. T cells from neonates do not respond to PPD in our system, while M phi from neonates are able to present PPD to T cells from HLA-D matched sensitized adults. It is also demonstrated that the previously reported HLA-D restriction of T cell/M phi cooperation is not due to T cell mediated killing of allogeneic macrophages in vitro.
D002478:Cells, Cultured; D006680:HLA Antigens; D006801:Humans; D007231:Infant, Newborn; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D014373:Tuberculin / Q000494:pharmacology*
D006680:HLA Antigens; D014373:Tuberculin