title: Perch muscle parvalbumin: general characterization and magnesium-binding properties.
abstract: 1. Parvalbumin exists in two major forms, amounting to 2.5 and 3.2 g per kg of fresh muscle. 2. The composition divergence index between both forms, calculated from their amino acid composition, indicates 81% sequence identity; however both isoparvalbumins are immunologically distinct. 3. Beside Ca2+, perch parvalbumin binds 2 g atoms Mg2+ per mol with a dissociation constant of 10(-5) M. 4. Determination of the affinity for magnesium is of particular importance as there are indications that, in vivo, parvalbumin can remain in the Mg2+-state during the contraction-relaxation cycle instead of switching from the Ca2+-to the Mg2+-state and vice versa.
mesh_terms: D000818:Animals; D008274:Magnesium / Q000378:metabolism*; D009124:Muscle Proteins / Q000378:metabolism*; D009132:Muscles / Q000378:metabolism*; D010320:Parvalbumins / Q000302:isolation & purification / Q000378:metabolism*; D010471:Perches / Q000378:metabolism*; D010473:Perciformes / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D009124:Muscle Proteins; D010320:Parvalbumins; D008274:Magnesium