title: Modification by soluble antigen of the immune response to mycobacterial infection.
abstract: After inoculation with viable BCG organisms, spleen cells from mice developed cellular immune reactivity to purified protein derivative antigen by day 10, which declined by day 25; after rechallenge on day 25, a vigorous secondary response rapidly developed (by day 29). The inclusion of autochthonous serum (from the BCG-infected mice) in the in vitro cellular immune reactivity tests altered this pattern considerably: the primary response was shortened, and the secondary response totally abated, suggesting the presence of blocking factors active against BCG-specific cellular immune reactivity in the sera of infected animals. Subsequent experiments indicated that BCG-specific serum blocking factor activity could be generated in the serum of control mice by the intravenous inoculation of purified protein derivative.
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial; D001500:BCG Vaccine; D002464:Cell Migration Inhibition; D004306:Dose-Response Relationship, Immunologic; D007104:Immune Adherence Reaction; D007106:Immune Sera; D007111:Immunity, Cellular; D051379:Mice; D009164:Mycobacterium Infections / Q000276:immunology*; D009163:Mycobacterium bovis / Q000276:immunology*; D012995:Solubility; D013154:Spleen / Q000276:immunology; D014373:Tuberculin
chemical_list: D000942:Antigens, Bacterial; D001500:BCG Vaccine; D007106:Immune Sera; D014373:Tuberculin