The level of FC receptor leukocytes in primary and secondary skin allograft recipients.
DA (Ag-B4) rats were grafted with allogeneic Lewis (Ag-B1) skin on the region of the thorax drained by the left brachial and axillary lymph nodes. A DA isograft was placed on the right side of the thorax. From 1 to 14 days after grafting the rats were either given injections of colloidal carbon, killed, and spleen and lymph nodes were assayed for noncarbon-containing Fc rosette-forming leukocytes (RFL), or allowed to complete rejection of the graft. In order to assess secondary allograft rejection within the latter animals after the grafted areas was healed, a second Lewis allograft was placed in the same region as the first and another DA isograft on the right side. Compartmental Fc RFL were enumerated 2 to 16 days thereafter by rosette formation with erythrocyte-antibody. The regional lymph nodes of animals rejecting a primary allograft exhibited a significantly (P less than 0.05) higher level of Fc RFL compared with their contralateral controls. These higher levels were evident on days 6 to 8 and disappear by day 10. However, animals undergoing a secondary allograft response exhibited significant differences by day 4. A greater density of Fc RFL were also evident in cryostat sections of the graft bed of allografts as opposed to isografts.
D000818:Animals; D004912:Erythrocytes / Q000276:immunology; D006084:Graft Rejection; D007111:Immunity, Cellular; D007141:Immunoglobulin Fc Fragments; D007962:Leukocytes / Q000276:immunology*; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D051381:Rats; D016038:Skin Transplantation; D013601:T-Lymphocytes / Q000276:immunology; D014184:Transplantation, Homologous; D014185:Transplantation, Isogeneic
D007141:Immunoglobulin Fc Fragments