[Effect of fixation in Carnoy's solution on nucleic acid and protein concentration in the rabbit superior cervical sympathetic ganglion].
By means of biochemical techniques, the stability of RNA, DNA and protein contents has been demonstrated in the rabbit superior cervical sympathetic node fixed in Carnoy's solution for 2 hours at 4 degrees C. A 2 hour fixation in Carnoy's fluid at room temperature, results, however, in a considerable loss of RNA (21%). The loss of DNA and protein did not exceed 2% of the total amount of the fresh ganglion tissue. Fixation at a higher temperature (37%) increased the loss percentage protein and of nucleic acids: 3.1, 5.5 and 42%, for protein, DNA and RNA, resp.
D000818:Animals; D004247:DNA / Q000032:analysis*; D005725:Ganglia, Autonomic / Q000032:analysis*; D006652:Histological Techniques; D009419:Nerve Tissue Proteins / Q000032:analysis*; D012313:RNA / Q000032:analysis; D011817:Rabbits
D009419:Nerve Tissue Proteins; D012313:RNA; D004247:DNA
Vliianie fiksatsii v shidkosti Karnua na soderzhanie nukleinovykh kislot i belka v verkhnem sheĭnom simpaticheskom ganglii krolika.