title: Stability of polyadenylic and polyadenylated ribonucleic acids in radish (Raphanus sativus) seedlings.
abstract: The stability of polyadenylic acid and polyadenylated RNA was investigated in young radish (Raphanus sativus) seedlings. We first studied the decay of poly(A) content, using a [3H]poly(U) assay, following a complete block of transcription by cordycepin (200 microgram/ml). Two lifetime classes of polyadenylic acid have been determined in these seedlings: a short-lived component with a half-life of 30 min which represents 60% of poly(A) and a more stable component with varying half-lives of which the majority range from 4-10 h and a few are considerably longer. During this period rRNA was shown to decay linearly, taking about 41 h for half of this RNA to disappear. The life-time of the other moiety of polyadenylated-RNA was analysed by continuous labelling with [3H]uridine. We have been able to demonstrate that a significant part of the mRNA molecules turns over with a half-life similar to that of the more slowly turning-over poly(A). No evidence could be obtained for rapidly turning-over messenger RNA. Thus the rapidly turning over poly(A) could correspond to a poly(A) turn-over independent of the remainder of the sequence. When labelling was very long, an apparent steady-state was reached and we determined the polyadenylated RNA content of seedlings to be 2.2% of whole cell RNA. Finally, these results were compared with those previously obtained in studying early germination of radish embryo axes. In contrast with stored mRNA which is rapidly degraded following imbibition, part of the mRNA present in 22 h old seedlings is stable for several hours.
mesh_terms: D003839:Deoxyadenosines / Q000494:pharmacology; D007700:Kinetics; D010945:Plants, Edible / Q000032:analysis*; D011061:Poly A / Q000032:analysis*; D012313:RNA / Q000032:analysis* / Q000096:biosynthesis; D012639:Seeds / Q000032:analysis; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D003839:Deoxyadenosines; D011061:Poly A; D012313:RNA