Serotyping of Serratia marcescens: evaluation of Le Minor's H-immobilization test and description of three new flagellar H antigens.
The H-immobilization test of Le Minor for determining flagellar (H) antigens was evaluated and compared with tube and slide H-agglutination tests. The test proved specific and easy to perform, and titration end points were clearly discernible. The degree of serological cross-reactivity between H antigen reference strains of Serratia marcescens was low. Consequently, this test was adopted for routine serological analysis of H antigens, using unabsorbed rabbit immune anti-H sera. As a result of using this procedure, three new provisional H antignes, designated H14, H15, and H16, are proposed.
D000942:Antigens, Bacterial / Q000032:analysis; D003429:Cross Reactions; D005407:Flagella / Q000276:immunology*; D012703:Serotyping / Q000379:methods*; D012706:Serratia marcescens / Q000145:classification*
D000942:Antigens, Bacterial