Expression of human T-lymphocyte antigens by natural killer cells.
Rabbit antisera against human T-lymphocyte and B-lymphocyte antigens (anti-HTLA and anti-HBLA, respectively) were used to characterize natural killer (NK) cells mediating spontaneous cell-mediated cytotoxicity for myelogenous leukemia cell line K562 as measured in a 51Cr-release assay. NK activity of lymphocyte suspensions was abrogated by incubation of the suspensions in anti-HTLA plus complement but not by incubation in anti-HBLA plus complement. The effect of anti-HTLA was due to removal of NK cells rather than antigen-antibody complex inhibition of NK cells inasmuch as lytic complement was necessary. The results indicated that NK cells were HTLA+-HBLA- and thus supported the concept that NK cells are T-cells.
D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity; D000961:Antilymphocyte Serum / Q000494:pharmacology; D001402:B-Lymphocytes / Q000276:immunology; D002460:Cell Line; D003165:Complement System Proteins; D006680:HLA Antigens; D006801:Humans; D007113:Immunity, Innate; D066298:In Vitro Techniques; D007694:Killer Cells, Natural / Q000276:immunology*; D007942:Leukemia, Experimental / Q000276:immunology; D007951:Leukemia, Myeloid / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000961:Antilymphocyte Serum; D006680:HLA Antigens; D003165:Complement System Proteins