Some clinical and pharmacological applications of high-speed liquid chromatography.
Through specific examples, the versatility of HSLC in clinical and pharmacological studies has been demonstrated. These examples are not intended to restrict the vision of the reader, like that of the author, but on the contrary, it is hoped that those not yet familiar with the usefulness of HSLC will gain perspective concerning this technique in their own fields of interest. The virtually unlimited ability to separate cellular metabolites (or other organic compounds for that matter) by HSLC is a reality at this time; however, development of more selective, sensitive means of detection and positive identification of eluting components is needed, similar to that gained by coupling mass spectrometry with gas chromatography.
D000241:Adenosine / Q000097:blood; D000243:Adenosine Deaminase / Q000172:deficiency; D000818:Animals; D002624:Chemistry, Clinical; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004285:Dogs; D004798:Enzymes / Q000097:blood*; D004912:Erythrocytes / Q000201:enzymology; D006801:Humans; D007042:Hypoxanthines / Q000032:analysis; D007288:Inosine / Q000097:blood; D009150:Mustard Compounds / Q000097:blood; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000378:metabolism; D010634:Phenobarbital / Q000097:blood; D010672:Phenytoin / Q000097:blood; D012265:Ribonucleotides / Q000032:analysis
D004798:Enzymes; D007042:Hypoxanthines; D009150:Mustard Compounds; D004364:Pharmaceutical Preparations; D012265:Ribonucleotides; D007288:Inosine; D010672:Phenytoin; D000243:Adenosine Deaminase; D000241:Adenosine; D010634:Phenobarbital