title: [Penicillin amidase from E. coli. A comparative study of the stability of penicillin amidase immobilized by various means].
abstract: The properties of immobilized penicillinamidases prepared by different methods were studied. Immobilization of penicillinamidase was achieved by using the covalend binding ion exchange sorption, incorporation into gel and other procedures. The effect of the carrier type, purification level of the native enzyme and other factors on stability of the immobilized preparations was studied. In 4 cases significant stabilization of the enzyme in the immobilized state was observed, while in 3 cases destabilization was registered.
mesh_terms: D000581:Amidohydrolases / Q000494:pharmacology*; D004355:Drug Stability; D004800:Enzymes, Immobilized; D004926:Escherichia coli / Q000201:enzymology*; D005782:Gels; D007474:Ion Exchange; D007475:Ion Exchange Resins / Q000494:pharmacology; D008722:Methods; D010399:Penicillin Amidase / Q000302:isolation & purification / Q000494:pharmacology*; D011485:Protein Binding
chemical_list: D004800:Enzymes, Immobilized; D005782:Gels; D007475:Ion Exchange Resins; D000581:Amidohydrolases; D010399:Penicillin Amidase
vernacular_title: Penitsillinamidaza iz E. coli. Sravnitel'noe izuchenie stabil'nosti penitsillinamidazy, immobilizovannoĭ razlichnymi sposobami