The reaction of cells with anti-actin sera in relation to the amount of cellular actin.
The staining pattern of anti-actin sera on various cells smeared on glass was compared to the relative amount of cellular actin estimated by SDS-polyacrylamide-gel electrophoresis with subsequent scanning of the gel. Although the cells showed a varying stainability the actin content was fairly constant. Thus, the staining differences reflected changes in the organization of cellular actin rather than actual differences in the amount of actin.
D000199:Actins / Q000032:analysis* / Q000276:immunology; D000818:Animals; D000906:Antibodies; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D007739:L Cells / Q000032:analysis; D008228:Lymphoma, Non-Hodgkin / Q000032:analysis; D051379:Mice; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes / Q000032:analysis; D013950:Thymus Gland / Q000032:analysis
D000199:Actins; D000906:Antibodies