New mini-ColE1 as a molecular cloning vehicle.
A new mini-ColE1 plasmid, designated pAC105, was isolated. It has a molecular weight of 1.6 X 10(6) and carries information for its self-replication as well as information for conferring colicin E1 immunity upon its host. Furthermore, pAC105 undergoes replication in the presence of chloramphenicol even when a foreign deoxyribonucleic acid (pSC101) is inserted into its single EcoRI restriction site. Studies in minicell-producing strains demonstrate that pAC105 codes for only two or three polypeptides of low molecular weight. The advantages of using it as a molecular cloning vehicle are discussed.
D001426:Bacterial Proteins / Q000096:biosynthesis; D002701:Chloramphenicol / Q000494:pharmacology; D003087:Colicins / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000096:biosynthesis; D004270:DNA, Circular / Q000096:biosynthesis; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects; D005111:Extrachromosomal Inheritance; D005796:Genes; D010957:Plasmids; D012293:Rifampin / Q000494:pharmacology
D001426:Bacterial Proteins; D003087:Colicins; D004269:DNA, Bacterial; D004270:DNA, Circular; D002701:Chloramphenicol; D012293:Rifampin