Isolation of Escherichia coli precursor tRNAs containing modified nucleoside Q.
Affinity chromatography based on the complex formation of the modified nucleoside Q with boronic acid has been applied to the isolation of specific tRNA precursors containing this modified nucleoside. When [32P]RNA isolated from an Escherichia coli strain containing a thermolabile ribonuclease P was chromatographed on dihydroxyboryl-substituted cellulose, the precursors for asparagine, aspartate, histidine, and tyrosine tRNA were specifically retained. All precursors were monomeric. The nucleotide sequences of four asparagine tRNA precursors were determined.
D001483:Base Sequence; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism*; D006151:Guanosine / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification; D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides / Q000032:analysis; D010179:Pancreas / Q000201:enzymology; D012343:RNA, Transfer / Q000096:biosynthesis / Q000302:isolation & purification; D012260:Ribonucleases; D014158:Transcription, Genetic
D009843:Oligoribonucleotides; D006151:Guanosine; D012343:RNA, Transfer; D012260:Ribonucleases