title: Replica-plating virulence assay for Agrobacterium tumefaciens.
abstract: Replica plating onto wounded tobacco leaves provides a convenient method for screening for avirulent mutants of Agrobacterium tumefaciens.
mesh_terms: D001431:Bacteriological Techniques; D009154:Mutation; D010941:Plant Tumors; D010947:Plants, Toxic; D012231:Rhizobium / Q000472:pathogenicity*; D014026:Nicotiana / Q000382:microbiology; D014774:Virulence