Escherichia coli membrane proteins with an affinity for deoxyribonucleic acid.
From the membrane fraction of Escherichia coli K-12 strain, four protein fractions (peaks I, IIa, IIb, and III) which have affinity for deoxyribonucleic acid (DNA) have been isolated. The molecular weights of these proteins are between 12,000 and 8,000. Only the peak III fraction contains a protein that binds preferentially to single-stranded DNA, whereas the others contain proteins that bind also to double-stranded DNA. The binding activity of the peak IIb protein is inhibited in the presence of polyuridylic acid. Peak I and peak IIa protein fractions behave like hydrophobic proteins.
D001426:Bacterial Proteins / Q000032:analysis* / Q000378:metabolism; D004269:DNA, Bacterial / Q000378:metabolism; D004926:Escherichia coli / Q000032:analysis* / Q000378:metabolism; D008274:Magnesium / Q000494:pharmacology; D008565:Membrane Proteins / Q000032:analysis* / Q000378:metabolism; D008970:Molecular Weight; D011072:Poly U / Q000494:pharmacology; D011189:Potassium Chloride / Q000494:pharmacology; D011485:Protein Binding / Q000187:drug effects; D013095:Spermidine / Q000494:pharmacology; D013347:Subcellular Fractions
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D008565:Membrane Proteins; D011072:Poly U; D011189:Potassium Chloride; D008274:Magnesium; D013095:Spermidine