Peanut agglutinin. II. Characterization of the Thy-1, Tla and Ig phenotype of peanut agglutinin-positive cells in adult, embryonic and nude mice using double immunofluorescence.
The Thy-1, Tla and Ig phenotype of peanut agglutinin (PNA)-binding cells was characterized in various strains of mice. In the thymus, PNA was found to bind principally but not exclusively to the Thy-1+ Tla + Ig-- steroid-sensitive cortical thymocytes. Thy1 + Tla -- Ig -- steroid-resistant cells are not labeled with PNA. In other lymphoid organs, PNA bound to a minority of T or null cells but generally not to B cells. During ontogeny, PNA + and PNA -- T lineage cells appear simultaneously in the liver at day 10 of gestation, in the thymus at day 11 and in the spleen at day 18. No evidence was found for a maturation from PNA + to PNA -- cells. Prethymocytes present in nude mice were also divided into a PNA + and PNA -- population.
D000818:Animals; D000954:Antigens, Surface / Q000032:analysis; D002965:Classification; D007519:Isoantigens / Q000032:analysis; D037102:Lectins / Q000276:immunology*; D008099:Liver / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology*; D008221:Lymphoid Tissue / Q000276:immunology; D051379:Mice; D008806:Mice, Inbred AKR / Q000276:immunology; D008814:Mice, Inbred NZB / Q000276:immunology; D008819:Mice, Nude / Q000276:immunology; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D013601:T-Lymphocytes / Q000276:immunology
D000954:Antigens, Surface; D007519:Isoantigens; D037102:Lectins; D011947:Receptors, Antigen, B-Cell