Analogs of methionyl-tRNA synthetase substrates containing photolabile groups.
Three photolabile analogs of substrates of methionyl-tRNA synthetase were synthesized. In one, the 4-thiouridine at the 8 position of E. coli tRNAfMet was alkylated with [14C]p-azidobromoacetanilide. In the second, [14C]p-azidobenzoic acid hydrazide was condensed with the 3'-terminal dialdehyde of periodate-oxidized Escherichia coli tRNAfMet. The modified tRNAs could be purified by chromatography on benzoylated DEAE-cellulose. The third photolabile compound was [3H]methioninyl-8-azido-adenosine 5'-phosphate, an analog of the methionyl adenylate intermediate in the aminoacylation reaction. Irradiation of each of these compounds in the presence of equimolar amounts of E. coli methionyl-tRNA synthetase of micrometer concentrations gave 5-15% crosslinking.
D000345:Affinity Labels; D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism; D004926:Escherichia coli; D008715:Methionine / Q000031:analogs & derivatives*; D008718:Methionine-tRNA Ligase / Q000378:metabolism; D008722:Methods; D009239:N-Formylmethionine; D010777:Photochemistry; D011485:Protein Binding; D012343:RNA, Transfer; D013329:Structure-Activity Relationship
D000345:Affinity Labels; D009239:N-Formylmethionine; D012343:RNA, Transfer; D008715:Methionine; D000604:Amino Acyl-tRNA Synthetases; D008718:Methionine-tRNA Ligase