A comparison of selected physical properties of hepatic sorbitol dehydrogenases [L-iditol: NAD oxidoreductases] from four mammalian species.
1. The sorbitol dehydrogenases [L-iditol: NAD oxidoreductase] from livers of cow, man, rat and sheep each possess molecular weights of about 140,000. The beef, rat and sheep liver enzymes are composed of subunits of molecular weight 40,000. 2. The sorbitol dehydrogenases from livers of these four species each possess an isoelectric point of 7.3. 3. The four enzyme preparations show identical mobilities upon disc-gel electrophoresis and yield a single band of enzymic activity. 4. Sorbitol dehydrogenase activity is activated by the presence of ampholines or by increasing ionic strengths, with maximal activation at about 0.5 M salt concentration. These factors may cause the Km for NAD to be lowered.
D000818:Animals; D002417:Cattle; D006801:Humans; D007526:Isoelectric Point; D007700:Kinetics; D007064:L-Iditol 2-Dehydrogenase / Q000302:isolation & purification* / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D008322:Mammals / Q000378:metabolism*; D008970:Molecular Weight; D011487:Protein Conformation; D051381:Rats; D012756:Sheep; D013045:Species Specificity; D013401:Sugar Alcohol Dehydrogenases / Q000302:isolation & purification*
D013401:Sugar Alcohol Dehydrogenases; D007064:L-Iditol 2-Dehydrogenase