Age-dependent changes in the electrophoretic mobilities of human blood lymphocytes.
The distribution of the anodic electrophoretic mobilities (EPM) of human peripheral blood lymphocytes was determined for lymphocytes isolated from umbilical cord blood and from blood of individuals 6 months to 93 years of age. The distribution was bimodal in infants up to 2 years of age and suggested a small percentage of cells with a mobility of 0.95 micrometer s-1 V-1 cm. this value was chosen to discriminate between low-mobility cells (LMCs) and high-mobility cells (HMCs). The relative percentage of LMCs increased from birth to 2 years and two types of LMCs could be distinguished. The distribution was unimodal and asymmetric in children and adults and nearly Gaussian in aged people. Substantial differences between the distributions of the lymphocyte EPMs were seen on comparison of the histograms for individuals of similar ages. The analysis of the distribution of the lymphocyte EPMs on cell suspensions enriched in, or depleted of T or B cells confirmed the mobility of most T cells to be higher than the mobility of most B cells, whatever the age of the individual. The distribution of lymphocyte EPMs determined in the same adult over a 6 year period showed minor variations.
D000293:Adolescent; D000328:Adult; D000367:Age Factors; D000368:Aged; D001402:B-Lymphocytes / Q000502:physiology*; D002465:Cell Movement; D002469:Cell Separation; D002648:Child; D002675:Child, Preschool; D004586:Electrophoresis; D006801:Humans; D007223:Infant; D008875:Middle Aged; D013601:T-Lymphocytes / Q000502:physiology*