title: Fractionation on lymphocyte surface antigens. I. Rapid method for eliminating labeled lipid from cell surface antigens iodinated by the lactoperoxidase catalysed reaction.
abstract: Fractionation of lactoperoxidase iodinated cell surface material on miniature DEAE-cellulose columns provided a rapid method for separating labeled lipid from cell surface antigens. The procedure also removed poorly solubilized aggregates yielding a labeled preparation which demonstrated stable, reproducible immunoprecipitation results. Using these fractionated antigens components tentatively designated as human 'T' cell specific antigens have been identified.
mesh_terms: D000941:Antigens / Q000032:analysis*; D002462:Cell Membrane / Q000276:immunology; D005591:Chemical Fractionation; D011232:Chemical Precipitation; D002848:Chromatography, DEAE-Cellulose; D006801:Humans; D007158:Immunologic Techniques; D007455:Iodine / Q000378:metabolism*; D007784:Lactoperoxidase / Q000378:metabolism*; D008055:Lipids; D008214:Lymphocytes / Q000276:immunology*; D010544:Peroxidases / Q000378:metabolism*; D013950:Thymus Gland / Q000276:immunology
chemical_list: D000941:Antigens; D008055:Lipids; D007455:Iodine; D007784:Lactoperoxidase; D010544:Peroxidases