The DNA content of micronuclei induced in mouse bone marrow by gamma-irradiation: evidence that micronuclei arise from acentric chromosomal fragments.
The DNA contents of 75 micronuclei found in mouse bone marrow 48 h after a 260 R dose of gamma-rays were measured individually and compared to 71 diploid G1 nuclei. The coefficient of variation for the diploid cells was 6.3%. The DNA content of micronuclei varied from 0.5 to 11.1% of the diploid G1 nuclei with a mean of 3.5%. These results are in agreement with the expectations based upon the hypothesis that micronuclei arise from acentric chromosomal fragments produced by random breakage of the mouse genome.
D000818:Animals; D001853:Bone Marrow / Q000528:radiation effects; D001854:Bone Marrow Cells; D002467:Cell Nucleus / Q000528:radiation effects*; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000528:radiation effects; D004247:DNA / Q000378:metabolism / Q000528:radiation effects*; D005260:Female; D005720:Gamma Rays; D005821:Genetic Techniques; D051379:Mice; D008810:Mice, Inbred C57BL; D009504:Neutrophils / Q000378:metabolism
D004247:DNA