Evaluation of the non-specific effects of catecholamine and serotonin neurotoxins by injection into the medial forebrain bundle of the rat.
Low doses of 6-hydroxydopamine (6-OHDA), 5,6-dihydroxytryptamine (5,6-DHT) and 5,7-dihydroxytryptamine (5,7-DHT) that have previously been shown to produce behavioral change following intracerebral infusion were injected into the medial forebrain bundle of the rat. This site contains serotonin (5-HT), norepinephrine (NE), and dopamine (DA) fibers whose anatomical locations have been described. Damage to these fiber systems was quantified by measuring depletion of telencephalic 5-HT, NE and DA. The effects of infusions of 6-OHDA, 5,6-DHT and 5,7-DHT were compared to the effects of unequivocally non-specific electrolytic lesions and copper sulfate infusions. Survival time was varied to evaluate the amount of regeneration that could be expected over periods from 8 to 60 days. Amine levels were found to be stable over the time period examined. With the doses used, evidence was found to support the position that non-specific damage caused by general cytotoxic effects of 6-OHDA and 5,7-DHT is minimized sufficiently to permit the acquisition of useful data on the function of central catecholamine and indoleamine systems.
D015115:5,6-Dihydroxytryptamine / Q000494:pharmacology*; D015116:5,7-Dihydroxytryptamine / Q000494:pharmacology*; D000818:Animals; D001923:Brain Chemistry / Q000187:drug effects; D002395:Catecholamines / Q000502:physiology*; D004102:Dihydroxytryptamines / Q000494:pharmacology*; D006892:Hydroxydopamines / Q000494:pharmacology*; D007267:Injections; D008297:Male; D008474:Medial Forebrain Bundle / Q000502:physiology*; D009434:Neural Pathways / Q000502:physiology*; D009498:Neurotoxins / Q000008:administration & dosage / Q000494:pharmacology; D051381:Rats; D012701:Serotonin / Q000502:physiology*
D002395:Catecholamines; D004102:Dihydroxytryptamines; D006892:Hydroxydopamines; D009498:Neurotoxins; D015116:5,7-Dihydroxytryptamine; D012701:Serotonin; D015115:5,6-Dihydroxytryptamine