Release factor binding to ribosome requires an intact 16 S rRNA 3' terminus.
Cloacin DF12 cleavage of Escherichia coli f[3H]MettRNA-AUG-ribosome complexes affects this substrate for in vitro peptide chain termination. Codon-directed release factors' (RF) 1 and 2 release of f[3H]methionine is inhibited by cloacin. Since cloacin inhibits RF1 and -2 binding to ribosomes but not RF-directed f[3H]methionine release from f[3H]met-tRNA-AUG-ribosome complexes when reactions contain 20% ethanol, we conclude that cloacin DF 13 inhibits formation of the termination codon recognition complex. Thus, cleavage of the 3'-OH 49-nucleotide sequence of the 16 S rRNA perturbs the codon-directed binding of RF to ribosomes.
D001426:Bacterial Proteins / Q000378:metabolism*; D001430:Bacteriocins / Q000494:pharmacology; D003062:Codon / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*; D008715:Methionine / Q000378:metabolism; D010443:Peptide Chain Termination, Translational / Q000187:drug effects; D010454:Peptide Termination Factors / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects; D012329:RNA, Bacterial / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism*
D001426:Bacterial Proteins; D001430:Bacteriocins; D003062:Codon; D010454:Peptide Termination Factors; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D012343:RNA, Transfer; D008715:Methionine