Quantitative studies o the activities of anti-human tonsil sera and anti-human spleen sera against B lymphocyte.
Anti-human B lymphocyte serum (AHBS) was obtained by absorbing rabbit anti-human tonsil serum (ATOS) or rabbit anti-human spleen serum (ASPS) with human red cells, liver, neutrophils, thymocytes and glutaraldehyde insolubilized human serum. Specificities of AHBS were checked by the cytotoxicity test, inhibition test of EAC-rosetting and inhibition of PWM responses. Raji cells of Burkitt's lymphoma originating from B-cells and acknowledged as non-immunoglobulin bearing on the cell surface were stained with AHBS according to the immunofluorescent technique. ATOS showed a higher cytotoxic titer against peripheral B-cells and inhibited EAC-rosetting stronger than ASPS did. On the other hand, macrophages in peripheral blood, Kupffer cells in the liver and certain cells of the thymus-medulla were stained with ASPA, but not with ATOS, even after intensively absorbing ASPS with thymocytes.
D000961:Antilymphocyte Serum / Q000032:analysis*; D001402:B-Lymphocytes / Q000276:immunology*; D003429:Cross Reactions; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis; D007158:Immunologic Techniques; D037102:Lectins / Q000494:pharmacology; D007945:Leukemia, Lymphoid / Q000276:immunology; D008213:Lymphocyte Activation; D014066:Palatine Tonsil / Q000276:immunology*; D013154:Spleen / Q000276:immunology*; D013950:Thymus Gland / Q000276:immunology
D000961:Antilymphocyte Serum; D007136:Immunoglobulins; D037102:Lectins