Isolation and preliminary characterization of 10-nm filaments from baby hamster kidney (BHK-21) cells.
A procedure for isolating 10-nm filaments as juxtanuclear caps from normal and colchicine-treated BHK-21 cells is described. These aggregates of 10-nm filaments retain their birefringence and their structural integrity. The major proteins comprising the filament preparations can be resolved as two bands on sodium dodecyl sulfate gels, corresponding to approximate molecular weights of 54,000 and 55,000.
D002460:Cell Line; D003078:Colchicine / Q000494:pharmacology; D007668:Kidney / Q000187:drug effects / Q000648:ultrastructure*; D008854:Microscopy, Electron; D008858:Microscopy, Phase-Contrast; D009940:Organoids
D003078:Colchicine