[Effect of phytohemagglutinin on T-lymphocytes isolated from human blood].
T--enriched lymphocyte fraction from normal donors was isolated from heparinized peripheral blood on Ficoll-Urotrast gradients. The mitogenic response of T-lymphocytes in culture was 3 times as high as that of unseparated mononuclear cells. The intensity of blast transformation to PHA did not correlate with the increase of T-lymphocytes in culture. These data suggest that cells of not T-origin may be the limiting factor during mitogenic response of T-lymphocytes to PHA.
D000328:Adult; D006801:Humans; D007958:Leukocyte Count; D008213:Lymphocyte Activation; D010835:Phytohemagglutinins; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000276:immunology*
D010835:Phytohemagglutinins
Deĭstvie fitogemaggliutinina na vydelennye iz krovi cheloveka T-limfotsity.