Studies on the kinetics of hemopoietic stem cells and immune responses to the hapten-carrier conjugate.
The correlation between the kinetics of hemopoietic stem cells and immune responses to the hapten-carrier conjugate was investigated. The numbers of both pluripotent stem cells (CFU-S) and myeloid stem cells (CFU-C) in the spleen from mice immunized with the hapten-carrier conjugate were significantly greater than those of the control and the activity of colony-stimulating factor (CSF) in the serum of these mice was markedly elevated. The supernatant of short-term incubation of splenic T lymphocytes from these mice, when stimulated with carrier protein, had high levels of both activities of CSF and helper T cell factors. The study by gel chromatography showed that these factors are similar m.w. substances of 35,000 to 45,000 daltons. But analysis by ion-exchange chromatography demonstrated that they do not have identical biochemical properties. The present studies suggest that biologically active factors produced by T cells stimulated with carrier protein may induce the enhancing effect on the proliferation and differentiation of hemopoietic stem cells and immune responses to the hapten-carrier conjugate.
D000818:Animals; D000917:Antibody Formation; D002352:Carrier Proteins / Q000276:immunology*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003115:Colony-Stimulating Factors / Q000097:blood; D004136:Dinitrobenzenes / Q000276:immunology; D005260:Female; D006241:Haptens / Q000276:immunology*; D006412:Hematopoietic Stem Cells / Q000276:immunology*; D006433:Hemocyanins / Q000276:immunology; D007700:Kinetics; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D002352:Carrier Proteins; D003115:Colony-Stimulating Factors; D004136:Dinitrobenzenes; D006241:Haptens; D006433:Hemocyanins