Stimulation of T-independent antibody responses by hapten-lipopolysaccharides without repeating polymeric structure.
The murine immune response to a haptenated lipopolysaccharide (LPS) lacking repeating oligosaccharide determinants was studied. The LPS was extracted from a rough strain of bacteria (Salmonella minnesota R595) and chemically haptenated with either trinitrophenol or fluorescein isothiocyanate. These preparations of hapten-R595 LPS were shown to be immunogenic. Furthermore, the immune response to the hapten was demonstrated to occur independent of T cells and was not merely the result of enhanced polyclonal B-cell activation. The capacity of such hapten-LPS conjugates without repeating polymeric structures to stimulate T-independent antibody responses provides information on the molecular requirements for the activation of murine B lymphocytes.
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D000942:Antigens, Bacterial; D001402:B-Lymphocytes / Q000276:immunology*; D005260:Female; D006241:Haptens; D008070:Lipopolysaccharides / Q000032:analysis / Q000276:immunology*; D008297:Male; D051379:Mice; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D012475:Salmonella / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D006241:Haptens; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial