title: Amino acid sequence of the L-arabinose-binding protein from Escherichia coli B/r.
abstract: The amino acid sequence of the L-arabinose-binding protein of Escherichia coli B/r was determined by sequenator analyses of reduced and S-pyridylethylated L-arabinose-binding protein and fragments derived by chemical and enzymatic cleavage of the native protein. The fragments were the products of cleavage by cyanogen bromide. BNPS-skatole, hydroxylamine, mild acid hydrolysis, limited trypsin digestion, chymotrypsin subdigestion, and subdigestion with Staphylococcus aureus protease V8. The COOH-terminal sequence was determined using bovine carboxypeptidases A and B and amino acid analyses. The L-arabinose-binding protein was determined to contain 306 amino acid residues, the sequence of which is presented below.
mesh_terms: D000595:Amino Acid Sequence; D001089:Arabinose / Q000378:metabolism*; D001426:Bacterial Proteins; D002352:Carrier Proteins / Q000378:metabolism; D004926:Escherichia coli; D010446:Peptide Fragments
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D010446:Peptide Fragments; D001089:Arabinose