Lipopeptidophosphoglycan from Trypanosoma cruzi. Amide and ester-linked fatty acids.
Lipopeptidophosphoglycan, extracted from whole cells of epimastigote forms of Trypanosoma cruzi, has now been shown to contain 12.6% of fatty acids in addition to the previously identified content of neutral sugars (60%), glucosamine (0.8%), peptide (9.5%) and acid-hydrolyzable phosphate (2%). The main fatty acids are palmitic (6.9%) and lignoceric (4.6%) acids. Stearic (0.55%), oleic (0.15%) and myristic (0.18%) acids were also found. One third of the fatty acids are bound in the lipopeptidophosphoglycan as esters (14 mmol%) and two thirds as amides (28 mmol%). Lignoceric acid was found to be bound only as amide. Two ninhydrin-positive compounds, obtained by chloroform extraction of a total acid hydrolysate of the lipopeptidophosphoglycan, were tentatively identified as sphingosine bases.
D000577:Amides / Q000032:analysis; D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002849:Chromatography, Gas; D004591:Electrophoresis, Polyacrylamide Gel; D005227:Fatty Acids / Q000032:analysis; D006898:Hydroxylamines; D009943:Organophosphorus Compounds / Q000032:analysis; D010457:Peptidoglycan / Q000031:analogs & derivatives* / Q000302:isolation & purification; D010743:Phospholipids / Q000302:isolation & purification*; D014349:Trypanosoma cruzi / Q000032:analysis*
D000577:Amides; D002241:Carbohydrates; D005227:Fatty Acids; D006898:Hydroxylamines; D009943:Organophosphorus Compounds; D010457:Peptidoglycan; D010743:Phospholipids; C014643:lipopeptidophosphoglycan