A solid-phase radioimmunoassay for polyhedron protein from baculoviruses.
A solid-phase radioimmunoassay has been developed for detecting polyhedron protein of Wiseana spp. nuclear polyhedrosis virus (W.NPV) using antigen fixed to plastic microtiter wells. The direct assay using 125I-labeled rabbit immunoglobulins could detect 0.2 microng of polyhedron protein, and the indirect method using 125I-labeled sheep antirabbit immunoglobulins could detect 0.05 microng of polyhedron protein. Antibodies against W.NPV polyhedron protein could be detected using a competition assay with labeled and unlabeled antibodies, providing a method suitable for rapid and quantitative screening of sera.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D001717:Birds / Q000276:immunology; D005069:Evaluation Studies as Topic; D006801:Humans; D007304:Insect Viruses / Q000032:analysis*; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods*; D012756:Sheep / Q000276:immunology; D014764:Viral Proteins / Q000032:analysis*
D000914:Antibodies, Viral; D014764:Viral Proteins