[The occurrence of cross-reactive antigens in myocardial tissue and in streptococcus pyogenes. IV. Cytotoxic effect of lymphocytes sensitized in vivo to group A streptococci on primary cultures of heart muscle cells (author's transl)].
The cytotoxic effect of rat lymphocytes in vivo sensitized to group A streptococci, rat heart tissue or streptococcal M proteins was investigated in primary cultures of beating rat heart cells. Cytotoxicity was found in 75 to 82% of heart cell cultures in contact with lymphocytes sensitized to streptococcal whole cells or lysates and in 100% of those exposed to lymphocytes sensitized to rat heart tissue. Lymphocytes sensitized to M protein exert a cytopathic effect only in 8 and 12% cultures, respectively, and to a much more limited extent. Controls were affected in 6%. The cytotoxicity test seems to be useful to examine the presence of cross-reactive antigens within streptococcal vaccines.
D000818:Animals; D000941:Antigens; D000942:Antigens, Bacterial; D002478:Cells, Cultured; D003429:Cross Reactions; D003601:Cytotoxicity Tests, Immunologic; D007114:Immunization; D008214:Lymphocytes / Q000276:immunology; D009206:Myocardium / Q000166:cytology / Q000276:immunology*; D051381:Rats; D013297:Streptococcus pyogenes / Q000276:immunology
D000941:Antigens; D000942:Antigens, Bacterial
Das Vorkommen kreuzreaktiver Antigens in Herzmuskelgewebe und Streptococcus pyogenes. IV. Zygotoxischer Effekt von Lymphozyten auf Primrkulturen von Herzmuskelzellen nach In-vivo-Sensibilisierung mit Streptokokken der Gruppe A.