A microtiter solid phase radioimmunoassay  for detection of the human reovirus-like agent in stools.
The development of a microtiter solid-phase radioimmunoassay for detection of the human reovirus-like (RVL) agent is described. The test is simple to perform and uses small amounts of reagents; a large number of specimens can rapidly be tested in a single test. Both filtered and unfiltered stool suspensions can be employed. The test was as sensitive as immune electron microscopy, and with an appropriate blocking test, was specific for the human RVL agent.
D000914:Antibodies, Viral; D000956:Antigens, Viral / Q000032:analysis*; D002675:Child, Preschool; D005243:Feces / Q000382:microbiology*; D005260:Female; D005759:Gastroenteritis / Q000209:etiology* / Q000276:immunology; D006801:Humans; D007158:Immunologic Techniques; D007223:Infant; D008297:Male; D008854:Microscopy, Electron; D011863:Radioimmunoassay / Q000379:methods*; D012087:Reoviridae / Q000276:immunology / Q000302:isolation & purification*
D000914:Antibodies, Viral; D000956:Antigens, Viral