title: Surface markers of porcine lymphocytes and distribution in various lymphoid organs.
abstract: Three rosette test systems were developed for the classification of subpopulations of porcine lymphocytes and correlated with the presence or the absence of membrane immunoglobulins, as well as agg-P-IgG receptors, by means of various RFC depletion experiments. Among the ED-RFC negative cells, PBL with surface immunoglobulins (SIg), at 4 degrees C, can be subdivided into two about equal populations: (1) One half of these cells have surface stable SIg (B cells) and mu determinants; they possess C3b receptors leading to the formation of rosettes with complement-coated zymosan particles (ZC). (2) The other half have surface labile Ig when the cells are washed at 37 degrees C (L-cells); they possess Fc receptors as detected by EA-RFC, i. e. pig-IgG-coated ox RBC. Receptors for Fc-agg, as detected by agg-P-IgG, were primarily shown on B cells. ZC and EA-RFC are in the highest tissue concentration, in bone marrow and spleen, respectively, and more numerous in adults than in newborns. Moreover, the number of EA-RFC was twofold higher than that of ZC-RFC in spleen, mesenteric lymph node and cisterna chyli. This latter organ was the only one without null cell.
mesh_terms: D000375:Aging; D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D001853:Bone Marrow / Q000276:immunology; D002417:Cattle; D003911:Dextrans / Q000494:pharmacology; D004912:Erythrocytes / Q000276:immunology; D007074:Immunoglobulin G; D007739:L Cells / Q000276:immunology; D008198:Lymph Nodes / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology*; D008643:Mesentery / Q000276:immunology; D011947:Receptors, Antigen, B-Cell; D011961:Receptors, Fc / Q000276:immunology*; D012397:Rosette Formation; D012756:Sheep; D013154:Spleen / Q000276:immunology; D013552:Swine; D013897:Thoracic Duct / Q000276:immunology
chemical_list: D003911:Dextrans; D007074:Immunoglobulin G; D011947:Receptors, Antigen, B-Cell; D011961:Receptors, Fc