II. Mechanism of increased colony formation of CFU-S and CFU-C after in vivo PHA administration in mice.
In vivo administration of 3 mg PHA-M results in an increase in spleen colonies due to an augmented seeding of CFU-S into the spleen. An increase in the number of CFU-S in the developing nine-day spleen colonies also occurred together with a slight increase in the number of CFU-S in DNA-S phase 24 hours after PHA-M administration. The increased colony formation was due to an increased number of CFU-C from the early culture period onwards but could not be explained by an increase in the number of CFU-C in DNA/S phase. The release of colony-stimulating substances was proved but this only partially accounted for the observed rise in bone marrow CFU-C.
D000818:Animals; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D003115:Colony-Stimulating Factors / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D051379:Mice; D010835:Phytohemagglutinins / Q000494:pharmacology*; D013154:Spleen / Q000166:cytology; D013997:Time Factors
D003115:Colony-Stimulating Factors; D010835:Phytohemagglutinins; D004247:DNA