[Characterization of staphylococci in microtiter-plates (author's transl)].
The conventional methods for the demonstration of coagulase, clumping-factor, fermentation of mannitol, protein A, alpha-, beta-, and delta-hemolysins and acid phosphatase were adapted to a microtitersystem (MTS). This led to a considerable saving in working-time and reagents. The biochemical reactions could be distinctly evaluated in the MTS (fig. 1). In all qualitative determinations there was complete agreement between the results obtained by MTS and the conventional procedures. In the quantitative endpoints there were only minor deviations (fig. 2,3,4). Thus, the MTS appeared to be a suitable procedure for the characterization of pathogenic staphylococci at a large scale.
D000373:Agglutinins / Q000032:analysis; D000818:Animals; D001431:Bacteriological Techniques; D002417:Cattle; D003030:Coagulase / Q000032:analysis; D006460:Hemolysin Proteins / Q000032:analysis; D006801:Humans; D013211:Staphylococcus aureus / Q000032:analysis / Q000145:classification*
D000373:Agglutinins; D003030:Coagulase; D006460:Hemolysin Proteins
Charakterisierung von Staphylokokken in Mikrotitrierplatten