Strikingly different effects of dinitrobenzene sulfonic and trinitrobenzene sulfonic acids on various subpopulations of murine lymphoid cells.
Different changes according to T- or B-cell origin occur in dinitrophenyl (DNP)- and trinitrophenyl (TNP)-modified lymphoid cells. The electrophoretic mobility (EPM) or nylon-wool adherent splenic cells (B cell-enriched) modified by TNP and DNP and that of nylon-wool non-adherent splenic cells (T cell-enriched) and thymic cells modified by TNP increase respectively as follows: +24%, +20%, + 6%, and +16%. A relation has been found between these EPM amounts and amino groups which are positively charged on cell surface. The EPM decreases in the case of nylon-wool non-adherent splenic cells (T cell-enriched, -9%) and thymic cells modified by DNP (-4%). The hydrophobic fluorescent probe 1-anilino-8-naphthalene sulphonate used to study conformational changes of cells revealed a slight fluorescence decrease for TNP-modified cells (-11% for thymic cells, -38% for T cells of spleen, and -66% for B cells of spleen), and a fluorescence increase for DNP-modified cells. This fluorescence increase is more important for spleen T cells (+130%) and thymic cells (+233%) than for spleen B cells (+30%).
D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects*; D002462:Cell Membrane / Q000187:drug effects; D004136:Dinitrobenzenes / Q000494:pharmacology*; D004586:Electrophoresis; D005260:Female; D051379:Mice; D008809:Mice, Inbred C3H; D009578:Nitrobenzenes / Q000494:pharmacology*; D013050:Spectrometry, Fluorescence; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000187:drug effects*; D014302:Trinitrobenzenesulfonic Acid / Q000494:pharmacology*
D004136:Dinitrobenzenes; D009578:Nitrobenzenes; D014302:Trinitrobenzenesulfonic Acid