Interaction of ascorbate with the radioprotective effect of mercaptoethylamin. An exploratory study in mice, whole animals and cell cultures.
The injection of ascrobate together with cysteamine (beta-mercaptoethylamin or MEA) was shown to cause a partial reversion of the radioprotective action of MEA in mice, and simultaneously of the suppressive action of MEA on RNA synthesis in bone marrow cells. In mouse spleen lymphocytes stimulated by concanavalin A in vitro, MEA and ascorbate exhibited a strong antagonism, neutralizing each other's inhibitory action on RNA synthesis. The latter effect failed to appear after chelation of trace metals, and it is indicated that the ability of ascorbate to counteract the effects of MEA on radiosensitivity and metabolism requires the formation of oxidized products, probably monodehydroascorbate, in agreement with previous observations on bacteria.
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology*; D001853:Bone Marrow / Q000528:radiation effects*; D002478:Cells, Cultured; D003543:Cysteamine / Q000494:pharmacology*; D004336:Drug Antagonism; D007928:Lethal Dose 50; D008297:Male; D051379:Mice; D011837:Radiation-Protective Agents; D013601:T-Lymphocytes / Q000528:radiation effects*; D014529:Uridine / Q000378:metabolism
D011837:Radiation-Protective Agents; D003543:Cysteamine; D001205:Ascorbic Acid; D014529:Uridine