[Study of a "RFA" locus coding for cell wall lipopolysaccharide core biosynthesis in "Shigella flexneri" F6S (author's transl)].
The rfa-7 lipopolysaccharide core mutation carried by the R mutant F680, derived from Shigella flexneri F6S serotype 5b, has been mapped by conjugation and transduction experiments. The results show a chromosomal distance of about 1 min between rfa-7 and mtl. Such a position would be similar to those of rfa genes in Escherichia coli K12 and Salmonella typhi-murium LT2. Conjugational transfer of E. coli K12 rfa+ genes to mutant F680 restored S. flexneri O-specificity. Chemical analyses performed on the lipopolysaccharide of such a rfa+ hybrid suggest that this strain can attach the S. flexneri serotype 5b O-specific polysaccharide to the E. coli K12 core.
D002473:Cell Wall / Q000032:analysis; D003227:Conjugation, Genetic; D004926:Escherichia coli; D005827:Genetics, Microbial; D008070:Lipopolysaccharides / Q000032:analysis / Q000096:biosynthesis*; D009154:Mutation; D011135:Polysaccharides, Bacterial / Q000032:analysis / Q000096:biosynthesis*; D012763:Shigella flexneri; D014161:Transduction, Genetic
D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial
Etude d'un locus génétique RFA impliqué dans la biosynthèse du "core" du lipopolyoside de la paroi de Shigella flexneri F6S