Mechanism of T-cell help in the immune response to soluble protein antigens. I. Evidence for in situ generation and action of T-cell-replacing factor during the anamnestic response to dinitrophenyl keyhole limpet hemocyanin in vitro.
The involvement of a nonantigen-specific T-helper factor in the anamnestic immune response to dinitrophenyl keyhole limpet hemocyanin is demonstrated employing cultures of unseparated spleen cell populations. In such cultures of primed and boosted spleen cells, a good IgG anti-DNP response could be obtained if the hapten was presented on a heterologous carrier, provided that the homologous carrier was added simultaneously. T-cell depletion and reconstitution experiments show that such a factor, presumably identical with T-cell-replacing factor is produced by primed helper cells upon rechallenge and helps primed B cells, stimulated by soluble heterologous carrier hapten conjugates, to become IgG-secreting cells.
D000818:Animals; D000921:Antibody-Producing Cells; D000941:Antigens; D000961:Antilymphocyte Serum; D001402:B-Lymphocytes / Q000276:immunology; D002478:Cells, Cultured; D004136:Dinitrobenzenes / Q000276:immunology; D005260:Female; D006433:Hemocyanins / Q000276:immunology; D006462:Hemolytic Plaque Technique; D007074:Immunoglobulin G; D007156:Immunologic Memory; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D010047:Ovalbumin / Q000276:immunology; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
D000941:Antigens; D000961:Antilymphocyte Serum; D004136:Dinitrobenzenes; D007074:Immunoglobulin G; D010047:Ovalbumin; D006433:Hemocyanins