Establishment of serum based essentiall free of proteolytic activity for the culture of mouse pancreatic islets.
The present study demonstrates that a) serum based culture medium degrades 125I inhibits its proteolytic activity leading to the recovery of more insulin secreted by islets cultured in the presence of high glucose concentration alone or with glucagon; c) aprotinin also favoured the accumulation of secreted insulin by protecting the hormone from a residual degradative capacity of the hear treated serum.
D000818:Animals; D007611:Aprotinin / Q000494:pharmacology; D001769:Blood; D002417:Cattle; D002478:Cells, Cultured; D003470:Culture Media; D005260:Female; D006358:Hot Temperature; D007328:Insulin / Q000378:metabolism; D007515:Islets of Langerhans / Q000378:metabolism*; D007700:Kinetics; D051379:Mice; D010447:Peptide Hydrolases / Q000378:metabolism
D003470:Culture Media; D007328:Insulin; D007611:Aprotinin; D010447:Peptide Hydrolases