Role of soluble cytotoxic factor in concanavalin A-induced cellular cytotoxicity to rabbit erythrocytes.
A new sensitive and simplified method for detecting concanavalin A-induced cellular cytotoxicity was developed by using 51Cr-labeled rabbit erythrocytes as target cells, and a soluble cytotoxic factor (SCF) to rabbit erythrocytes was demonstrated with this system. From morphological studies, this factor appeared to play a major role in cellular cytotoxicity. The cytotoxic activity of SCF was removed by absorption with rabbit erythrocytes. Furthermore, the target cells pretreated with SCF and washed were subsequently destroyed. These results may indicate that SCF exerts its effect through binding to cellular receptors. Some monosaccharides showed inhibitory effects on the cytolysis of rabbit erythrocytes by SCF and on the adsorption of SCF activity to them. The significance of these findings in elucidation of the action of SCF was discussed.
D000818:Animals; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D003602:Cytotoxicity, Immunologic; D004912:Erythrocytes / Q000276:immunology*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology*; D008233:Lymphotoxin-alpha / Q000276:immunology* / Q000378:metabolism; D009005:Monosaccharides / Q000494:pharmacology; D011817:Rabbits / Q000276:immunology; D051381:Rats; D011971:Receptors, Immunologic / Q000378:metabolism
D008233:Lymphotoxin-alpha; D009005:Monosaccharides; D011971:Receptors, Immunologic; D003208:Concanavalin A