The specificity of the chemical modification of N6-delta 2-(isopentenyl)adenosine in purified yeast tRNA Ser.
The specific modification of N6-delta 2-(isopentenyl)adenosine in purified tRNA Ser yeast by mild treatment with KMnO4 and I2 was studied. N6-delta 2-(isopentenyl)adenosine in tRNA SER is specifically modified by iodination, providing us with a suitable method for the quantitative determination of N6-delta 2-(isopentenyl)adenosine in tRNA was found to contain 114 +/- 8 pmol/A260nm unit of N6-delta 2-(isopentenyl)adenosine and gave three labelled fractions on an RPC-5 column. The product obtained after KMnO4 treatment of tRNA Ser was not homogeneous. The enzymatic "reisopentenylation" of KMnO4-treated tRNA Ser resulted in the regeneration of only traces of the original molecule(s). Most of them had been damaged either by the KMnO4 treatment or in the incubation mixture used for "reisopentenylation".
D000241:Adenosine / Q000031:analogs & derivatives*; D002848:Chromatography, DEAE-Cellulose; D002852:Chromatography, Ion Exchange; D002854:Chromatography, Paper; D012343:RNA, Transfer / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000032:analysis*; D012694:Serine
D012694:Serine; D012343:RNA, Transfer; D000241:Adenosine