Kinetic measurement of alpha-amylase with a centrifugal analyzer.
We compared results of an enzymic assay for alpha-amylase (EC 3.2 1.1), adapted to a centrifugal analyzer (GEMSAEC), with results by the manual dyed-starch (Phadebas) amylase assay. We assessed linearity and precision, and the stability of the reagent. The kinetic procedure is rapid, precise, requires only 25 microliter of serum, and thus is evidently suited for both pediatric and emergency use.
D000681:Amylases / Q000097:blood*; D001322:Autoanalysis; D002498:Centrifugation / Q000379:methods; D003124:Colorimetry / Q000379:methods; D006801:Humans; D007700:Kinetics; D000516:alpha-Amylases / Q000097:blood*
D000681:Amylases; D000516:alpha-Amylases