title: Lactate dehydrogenase isozymes in the spermatozoa of the domestic fowl, Gallus domesticus and turkey, Meleagris gallopavo.
abstract: 1. Electrophoresis of extracts of turkey spermatozoa for lactate dehydrogenase activity revealed the usual five tissue LDHs (LDH-1 to LDH-5). 2. The presence of LDH-X (the spermatozoan-specific isozyme) was not obvious. 3. Only one band was present on electrophoresis of fowl spermatozoan extracts and it coincided with LDH-1 (heart type). 4. Kinetic investigations, the use of inhibitors and the heat-stability test confirmed that the fowl spermatozoan LDH was probably LDH-1 and not LDH-X.
mesh_terms: D000818:Animals; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000032:analysis*; D008297:Male; D011200:Poultry / Q000378:metabolism*; D013094:Spermatozoa / Q000201:enzymology*; D014422:Turkeys / Q000378:metabolism*
chemical_list: D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase