Radioimmune double PEG precipitation technique for detecting complexed IgE.
The presence of IgE in immune complex-enriched fractions prepared by 3% polyethylene glycol (PEG) precipitation was studied. The dissolved PEG-insoluble fraction was incubated with [125I]anti-IgE antibodies an then precipitated again with 3% PEG. Radioactivity in the washed pellets was taken as indicating IgE present in immune complex form. A calibration curve was constructed using artificially prepared IgE-anti-IgE complexes. The method developed may be useful to detect other components present in immune complex fractions.
D000888:Antibodies, Anti-Idiotypic; D000936:Antigen-Antibody Complex; D001168:Arthritis / Q000097:blood; D001171:Arthritis, Juvenile / Q000097:blood; D001172:Arthritis, Rheumatoid / Q000097:blood; D011232:Chemical Precipitation; D004342:Drug Hypersensitivity / Q000097:blood; D006801:Humans; D007073:Immunoglobulin E / Q000302:isolation & purification*; D008180:Lupus Erythematosus, Systemic / Q000097:blood; D011092:Polyethylene Glycols
D000888:Antibodies, Anti-Idiotypic; D000936:Antigen-Antibody Complex; D007073:Immunoglobulin E; D011092:Polyethylene Glycols