Enzyme differentiation of lymphocyte subpopulations in sections of human lymph nodes, tonsils and periodontal disease.
Five enzymes have been studied to differentiate between T and B lymphocytes in sections of human lymph nodes, tonsils and chronic inflammatory periodontal disease. The presence of acid phosphatase, beta-glucuronidase N-acetyl-beta-glucosaminidase, non-specific esterase and fluoride-resistant esterase activity was determined histochemically. The results indicate that cells in the B area of both lymph nodes and tonsils are negative for enzyme activity, while those in the T area show a single intense granule of activity. These enzymes were unable to differentiate between T blasts, B blasts and plasma cells in the sections studied. The majority of the lymphoid cells in the lesions of chronic inflammatory periodontal disease are enzyme-negative and probably of B-cell origin.
D001402:B-Lymphocytes / Q000201:enzymology*; D004798:Enzymes / Q000378:metabolism; D006651:Histocytochemistry; D006801:Humans; D008198:Lymph Nodes / Q000276:immunology*; D014066:Palatine Tonsil / Q000276:immunology*; D010518:Periodontitis / Q000276:immunology*; D013601:T-Lymphocytes / Q000201:enzymology*
D004798:Enzymes