L-Phenylalanine ammonia-lyase and pisatin induction by 5-bromodeoxyuridine in Pisum sativum.
The substitution of the base analogue 5-bromodeoxyuridine (BrdU) for thymidine in the DNA of pea pods (Pisum sativum) induces or enhances the level of phenylalanine ammonia-lyase (PAL) and also induces the phytoalexin, pisatin, a product of the same metabolic pathway. Cordycepin, a polyadenylate inhibitor at the RNA level, is a potent inhibitor of pisatin synthesis. Kinetic studies on the inhibition of the PAL-pisatin production by hydroxyurea indicate that BrdU must be incorporated into DNA before any induction takes place. 5-Iododeoxyuridine is also an inducer while 5-fluorodeoxyuridine is ineffective when applied alone. BrdU is incorporated into the DNA of pea cells and the nuclei undergoes condensation just prior to the detection of the induced responses.
D000642:Ammonia-Lyases / Q000096:biosynthesis*; D001578:Benzopyrans / Q000096:biosynthesis*; D001973:Bromodeoxyuridine / Q000494:pharmacology*; D003839:Deoxyadenosines / Q000494:pharmacology; D004148:Dioxolanes / Q000096:biosynthesis; D004790:Enzyme Induction; D006918:Hydroxyurea / Q000494:pharmacology; D007700:Kinetics; D008855:Microscopy, Electron, Scanning; D010650:Phenylalanine Ammonia-Lyase / Q000096:biosynthesis*; D010944:Plants / Q000201:enzymology* / Q000648:ultrastructure; D036343:Pterocarpans; D000094162:Phytoalexins
D000642:Ammonia-Lyases; D001578:Benzopyrans; D001973:Bromodeoxyuridine; D003839:Deoxyadenosines; D004148:Dioxolanes; D006918:Hydroxyurea; D010650:Phenylalanine Ammonia-Lyase; D036343:Pterocarpans; C003161:pisatin; D000094162:Phytoalexins