title: Characterization of the role of mononuclear cell subpopulations in the in vitro lymphocyte proliferation assay.
abstract: Purified human mononuclear cell subpopulations have been evaluated in the in vitro lymphocyte proliferation assay. Monocyte-depleted mononuclear cells had reduced or absent responses to mitogens and antigens which could be restored to the original mononuclear cell response by addition of purified plastic adherent cells. Purified T lymphocytes obtained by density gradient centrifugation of E-rosette-forming cells demonstrated low but significant proliferative responses to mitogens, but no significant response to antigens. The addition of monocytes potentiated the response of purified T cells to mitogens and antigens, but did not fully reconstitute the original mononuclear cell response unless non-T lymphocytes were also present. It is concluded that mononuclear cell proliferation represents a complex mechanism of cellular interaction involving multiple subpopulations of cells.
mesh_terms: D000941:Antigens / Q000276:immunology; D002448:Cell Adhesion; D006801:Humans; D007962:Leukocytes / Q000276:immunology*; D008213:Lymphocyte Activation; D008264:Macrophages / Q000276:immunology*; D008934:Mitogens / Q000494:pharmacology; D009000:Monocytes / Q000276:immunology; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism
chemical_list: D000941:Antigens; D008934:Mitogens; D013936:Thymidine