Recovery of hematologic competence without engraftment following attempted bone marrow transplantation for aplastic anemia: Report of a case with diffusion chamber studies.
An 18-year-old male with severe aplastic anemia associated with hepatitis was prepared for bone marrow transplantation with cyclophosphamide (50 mg/kg/day) for 4 consecutive days. He then received 3.47 X 10(8) nucleated bone marrow cells per kilogram of body weight from his HL-A identical sister by intravenous infusion. Although subsequent karyotyping showed that engraftment was not achieved, prompt hematologic recovery ensued by the third week post-transplant. Diffusion chamber culture of the patient's bone marrow stroma, obtained prior to cyclophosphamide therapy, was carried out. A prompt increase in the number of cells in the chambers was observed. A differential cell count of the contents was similar to that seen when normal bone marrow is cultured in a similar manner.
D000293:Adolescent; D000741:Anemia, Aplastic / Q000097:blood / Q000150:complications / Q000628:therapy*; D001772:Blood Cell Count; D001853:Bone Marrow / Q000648:ultrastructure; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D002478:Cells, Cultured; D003520:Cyclophosphamide / Q000627:therapeutic use; D004900:Erythroblasts; D006098:Granulocytes; D006410:Hematopoiesis; D006505:Hepatitis / Q000150:complications; D006644:Histiocytes; D006801:Humans; D007165:Immunosuppression Therapy; D007275:Injections, Intravenous; D007621:Karyotyping; D008214:Lymphocytes; D008297:Male; D009000:Monocytes; D014184:Transplantation, Homologous
D003520:Cyclophosphamide