Discrimination between human T and B lymphocytes and monocytes by computer analysis of digitized data from scanning microphotometry. I. Chromatin distribution patterns.
Digitized images of Feulgen-stained normal human B and T lymphocytes and monocytes were analyzed by computer and microphotometry. The algorithms assessed the chromatin distribution patterns. A substantial number of parameters descriptive of the chromatin distributions were found to show statistically highly significant differences between B and T cells and monocytes. Although the effects of a number of biologic variables, such as cell cycle phase and immune stimulation, need to be carefully evaluated, the preliminary results indicate potential for automatic classification and subclassification of human B and T lymphocytes in normal and disease states.
D000328:Adult; D001402:B-Lymphocytes / Q000032:analysis*; D002843:Chromatin / Q000032:analysis*; D002965:Classification; D003201:Computers; D006801:Humans; D009000:Monocytes / Q000032:analysis*; D010783:Photometry; D013601:T-Lymphocytes / Q000032:analysis*
D002843:Chromatin