Effect of dimethyl sulphoxide on the expression of nitrogen fixation in bacteria.
Storage in dimethyl sulphoxide (DMSO) of Escherichia coli K12 hybrids carrying nif+ genes from Klebsiella pneumoniae can result in selection of a defective nitrogen-fixing phenotype. Similar results are obtained with E. coli K12 hybrids containing the nitrogen-fixing capacity from Rhizobium trifolii. DMSO appears to affect particular inner membrane proteins associated with energy metabolism in E. coli K12 and four chromosomal regions (chlD, chlG, his and unc) are associated with resistance to DMSO.
D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects* / Q000378:metabolism; D005796:Genes / Q000187:drug effects*; D006824:Hybridization, Genetic; D007711:Klebsiella pneumoniae; D009586:Nitrogen Fixation / Q000187:drug effects*; D012231:Rhizobium; D014161:Transduction, Genetic
D004121:Dimethyl Sulfoxide