title: Use of antibody-coated red cells for the sensitive detection of antigen and in rosette tests for cells bearing surface immunoglobulins.
abstract: Conditions for an improved chromic chloride method for the attachment of antibody to red cells are described. The method, which is applicable to whole Ig fractions as well as affinity-purified antibodies, is reproducible and has a sensitivity for detection of antigen in the nanogram range. The coated cells may be used in a rosette assay for the detection of cell surface-bound antigens.
mesh_terms: D000906:Antibodies; D000941:Antigens / Q000032:analysis*; D002857:Chromium; D004912:Erythrocytes / Q000276:immunology*; D007158:Immunologic Techniques; D066298:In Vitro Techniques; D008832:Microchemistry; D011947:Receptors, Antigen, B-Cell / Q000032:analysis*
chemical_list: D000906:Antibodies; D000941:Antigens; D011947:Receptors, Antigen, B-Cell; D002857:Chromium