Plasmid detection and sizing in single colony lysates.
A simple and contained procedure for the rapid assay of the presence and size of plasmids similar to Col El is described. Bacteria are picked from an agar plate with a toothpick, lysed with dodecyl sulfate and heat, and placed directly on an agarose gel for electrophoresis.
D003087:Colicins; D004269:DNA, Bacterial / Q000032:analysis*; D004270:DNA, Circular / Q000032:analysis; D004587:Electrophoresis, Agar Gel; D004926:Escherichia coli; D005111:Extrachromosomal Inheritance; D008722:Methods; D009690:Nucleic Acid Conformation; D010957:Plasmids
D003087:Colicins; D004269:DNA, Bacterial; D004270:DNA, Circular