The estimation of plasma valproate by gas-liquid chromatography.
A gas chromatographic method for the plasma assay of the anticonvulsant, sodium valproate, is described. Derivatization is not necessary. 200 microliter plasma are required for a single estimation. The method involves a chloroform extraction of valproate and the internal standard, cyclopentane carboxylic acid, from acidified plasma. Gas-liquid chromatography using the stationary phase 10% SP-216-PS gives complete separation of valproate and the internal standard in eight minutes. The limit of detection is 20 mumol valproate/1 plasma (equivalent to 40 pmol on column). This is well below the lower therapeutic plasma level. The between-run precision of the method indicates a variation for each sample within+/-3% of its mean value.
D002849:Chromatography, Gas; D006801:Humans; D008722:Methods; D014631:Valerates / Q000097:blood*; D014635:Valproic Acid / Q000097:blood*
D014631:Valerates; D014635:Valproic Acid