Synthesis of antithrombin III and alpha-1-antitrypsin by the perfused rat liver.
Livers isolated from control or turpentine-injected rats were perfused for 3 h with human red cells suspended in Krebs-Henseleit solution containing bovine serum albumin, dextran, glucose, heparin, cortisol, insulin, a mixture of 20 amino acids and [3H]leucine. Changes in the concentrations of antithrombin III and alpha-1-antitrypsin were evaluated by rocket immunoelectrophoresis using specific antisera, and incorporation of the 3H radioactivity into the total protein, albumin, antitrhombin III and alpha-1-antitrypsin in the perfusate was measured. The results indicate that both antithrombin III and alpha-1-antitrypsin are synthesized in the liver. Local inflammation induced in the liver donors moderately stimulated the synthesis of alpha-1-antitrypsin but it affected only marginally that of antithrombin III.
D000418:Albumins / Q000096:biosynthesis; D000818:Animals; D000991:Antithrombins / Q000096:biosynthesis*; D007930:Leucine / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D010477:Perfusion; D014176:Protein Biosynthesis; D051381:Rats; D014425:Turpentine / Q000494:pharmacology; D000515:alpha 1-Antitrypsin / Q000096:biosynthesis*
D000418:Albumins; D000991:Antithrombins; D000515:alpha 1-Antitrypsin; D007930:Leucine; D014425:Turpentine