The collaboration of T-cell subsets in the mitogenic stimulation of purified B-cell subpopulations.
A highly purified preparation of mouse B cells showed greatly decreased incorporation of [6-3H]-thymidine when stimulated with pokeweed Pa-1 mitogen or bacterial lipopolysaccharide compared with mouse splenic lymphocytes. This decreased stimulation was restored by the addition of purified T cells, but not macrophages. Nylon-adherent T cells exerted this helper activity only toward complement receptor-positive B cells (CR+B cells). whereas the helper activity of nylon-non-adherent T cells was effective only on complement receptor-negative B cells (CR-B cells). Since the helper activity of nylon-adherent T cells was completely abolished by the treatment with anti-Ia antiserum and complement but that of nylon-non-adherent T cells was not, it was assumed that Ia+T cells were helper cells for CR+B cells and Ia-T cells helper cells for CR-B cells. Moreover, these helper activities of both T-cell subsets were mediated by soluble factors, which were effective just before the onset of DNA synthesis of the corresponding B-cell subpopulations.
D000818:Animals; D001402:B-Lymphocytes / Q000145:classification*; D002448:Cell Adhesion; D002469:Cell Separation; D005260:Female; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation; D008211:Lymphocyte Cooperation; D051379:Mice; D008809:Mice, Inbred C3H; D008819:Mice, Nude; D008934:Mitogens / Q000494:pharmacology*; D011043:Pokeweed Mitogens / Q000494:pharmacology; D011951:Receptors, Complement; D013601:T-Lymphocytes / Q000145:classification*
D008070:Lipopolysaccharides; D008934:Mitogens; D011043:Pokeweed Mitogens; D011951:Receptors, Complement