Incorporation of 14C-amino acids into rat hypothalamus in vitro.
Biosynthesis of peptides in vitro in male rat hypothalamic fragments wasinvestigated by incubating the tissue with 14C-labelled leucine, proline and glycine. Depending on the amino acid used, 4 or 6 labelled peptides were extracted from the tissue fragments with organic solvent mixture. The incorporation of the label into peptides was time dependent. LH-RH and oxytocin thus synthesized were identified by thin layer chromatography and high voltage electrophoresis using cold LH-RH and oxytocin as standards.
D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D005998:Glycine / Q000378:metabolism; D007987:Gonadotropin-Releasing Hormone / Q000096:biosynthesis; D007031:Hypothalamus / Q000378:metabolism*; D007700:Kinetics; D007930:Leucine / Q000378:metabolism; D008297:Male; D010121:Oxytocin / Q000096:biosynthesis; D011392:Proline / Q000378:metabolism; D014176:Protein Biosynthesis; D051381:Rats
D000596:Amino Acids; D007987:Gonadotropin-Releasing Hormone; D010121:Oxytocin; D011392:Proline; D007930:Leucine; D005998:Glycine