Nuclear DNA synthesis in yeast and the effect of irradiation.
The synthesis of DNA can be measured in yeast by following the uptake of 5-bromodeoxy-uridine-5'-triphosphate in a mutant that utilizes deoxythymidine-5'-monophosphate; approximately 60 per cent of the DNA is synthesized semi-conservatively before replication stops. Neither ultraviolet light (U.V.), nor ionizing radiation stimulates repair-type synthesis. Based on the ability to detect small amounts of synthesis, it appears that fewer than ten bases are synthesized per pyrimidine dimer removed.
D002467:Cell Nucleus / Q000378:metabolism / Q000528:radiation effects; D003037:Cobalt Radioisotopes; D004247:DNA / Q000096:biosynthesis; D004261:DNA Replication / Q000528:radiation effects*; D005720:Gamma Rays; D012441:Saccharomyces cerevisiae / Q000378:metabolism / Q000528:radiation effects*; D014466:Ultraviolet Rays
D003037:Cobalt Radioisotopes; D004247:DNA