[In vivo and in vitro studies of phage T4 lysozyme mRNA translation].
Translation of phage T4 lysozyme mRNA is studied in vivo and in vitro. Polyribosomes, carrying growing lysozyme polypeptides, are found to be homogenous enough and to contain 6 ribosomes. Complete molecules of phage lysozyme, which possess an enzymatic activity and are similar to the native enzyme in its electrophoretic mobility in polyacrylamide gel, have been synthetized in vitro on RNA isolated from phage-infected cells. The efficiency of RNA translation in cell-free system is discussed on the model of synthesis of functionally active individual protein.
D002474:Cell-Free System; D003090:Coliphages / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism; D009113:Muramidase / Q000096:biosynthesis* / Q000378:metabolism; D011132:Polyribosomes / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D014764:Viral Proteins / Q000096:biosynthesis
D012333:RNA, Messenger; D014764:Viral Proteins; D009113:Muramidase
Izuchenie transliatsii mRNK lizotsima faga T4 in vivo i in vitro.