Rapid separation of mouse T and B lymphocytes using wheat germ agglutinin.
A separation procedure has been developed for mouse splenic T and B lymphocytes which is based on their differential agglutination by wheat germ agglutinin (WGA). In the presence of 50-100 micrograms/ml of WGA, multicellular aggregates are formed which are enriched in B cells. These aggregates can be separated from monodisperse T cells by gravity sedimentation and subsequently dissociated into single cells by treatment with N-acetylglucosamine (NAG). Immunocytochemical analyses and mitogenic assays indicate approximately 10-15% cross contamination of the resultant B and T cell fractions. The separation procedure is not only convenient and rapid but also allows the simultaneous recovery of viable T and B cells from the same spleen preparation.
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology*; D002469:Cell Separation / Q000379:methods*; D003208:Concanavalin A / Q000494:pharmacology; D037102:Lectins; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D037121:Plant Lectins; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000166:cytology*; D014908:Triticum
D037102:Lectins; D037121:Plant Lectins; D003208:Concanavalin A