Mixed agglutination in microtiter U-trays.
The principle of mixed agglutination with cell cultures was applied to those cells which could not be obtained as monolayer cell cultures. Artificial monolayers of freshly isolated cells from various tissues were prepared in poly-L-lysine-coated wells of a microtiter U-tray. By means of this technique, murine alloantigens, H-2 private specificities and Thy-1 antigens were demonstrated on cells from thymus, brain, spleen, kidney and liver. Ia.2 antigen was detected on splenocytes but not on thymocytes or fibroblast L cells. Evidence was also presented that HLA A and B locus antigens could be demonstrated on human thymocytes. Sensitivity of this test was shown to be at least 100 times higher than that of the standard lymphocytotoxicity test for detection of HLA antigens.
D000042:Absorption; D000372:Agglutination Tests / Q000379:methods*; D000818:Animals; D000961:Antilymphocyte Serum / Q000494:pharmacology; D002478:Cells, Cultured; D003601:Cytotoxicity Tests, Immunologic; D005260:Female; D006183:H-2 Antigens; D006680:HLA Antigens; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D007668:Kidney / Q000276:immunology; D008099:Liver / Q000276:immunology; D051379:Mice; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D051381:Rats; D011917:Rats, Inbred Lew; D011920:Rats, Inbred WF; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D000961:Antilymphocyte Serum; D006183:H-2 Antigens; D006680:HLA Antigens; D007106:Immune Sera