Quantitative determination of cross-linkage of bacteriophage DNA and protein by ionizing radiation.
Coliphage T7 was dissolved in tryptone broth and exposted to 60C gamma-radiation. Cross-linkage of DNA and protein of the virion was assayed using phenol-water countercurrent distribution. The results are interpreted in terms of a statistical model of cross-linkage and double-strand breaks. It was found that protein--DNA cross-links accumulate linearly with dose at a rate of o.74 X 10(-11) cross-links per rad per nucleotide pair, which is of the order of 5 per cent of the formation rate of double-strand breaks.
D003037:Cobalt Radioisotopes; D003090:Coliphages / Q000528:radiation effects*; D004279:DNA, Viral / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D014764:Viral Proteins / Q000528:radiation effects*
D003037:Cobalt Radioisotopes; D004279:DNA, Viral; D014764:Viral Proteins