title: Lymphocyte chimerism after bone marrow transplantation. Surface markers and in vitro function of donor and recipient lymphocyte subpopulations.
abstract: Specific HLA antibodies were used to eliminate donor and recipient cells, respectively, from lymphocyte suspensions prepared from the blood of a child who had been transplanted with bone marrow from an HLA-A- and HLA-B-incompatible, HLA-D-compatible donor. About 70% of the lymphocytes were of donor HLA type, the remaining of recipient type. The phytohemagglutinin-responsive lymphocytes were exclusively limited to the lymphocyte population carrying donor-type HLA antigens. Membrane immunofluorescence investigations of the donor and recipient populations showed a low percentage of IgM-positive lymphocytes in the donor population and an extremely high proportion of IgM-positive lymphocytes in the recipient population. About 90% of the donor lymphocytes were T cells, as judged by their capacity to form rosettes between sheep erythrocytes and T lymphocytes; no cells in the recipient cell population expressed this ability.
mesh_terms: D001402:B-Lymphocytes / Q000276:immunology; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D002470:Cell Survival; D002478:Cells, Cultured; D002648:Child; D003165:Complement System Proteins; D006680:HLA Antigens; D006650:Histocompatibility Testing; D006801:Humans; D007104:Immune Adherence Reaction; D007153:Immunologic Deficiency Syndromes / Q000628:therapy*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D013601:T-Lymphocytes / Q000276:immunology; D014184:Transplantation, Homologous
chemical_list: D006680:HLA Antigens; D011947:Receptors, Antigen, B-Cell; D003165:Complement System Proteins