A double-antibody radioimmunoassay for serum progesterone using progesterone-3-(O-carboxymethyl) oximino-[125I]-iodo-histamine as radioligand.
A reliable, convenient and economical radioimmunoassay (RIA) for serum progesterone has been established and tested. This procedure employs diethyl ether extraction followed by RIA utilizing rabbit anti-11 alpha-hydroxyprogesterone 11-hemisuccinyl-bovine serum albumin (progesterone-11 alpha-BSA) serum, progresterone-3-(O-carboxymethyl) oximino-[125I]-iodohistamine (progesterone-3-[125I]) as radioligand and goat anti-rabbit gamma globulin as second antibody. In conjunction with antiprogesterone-11 alpha-BSA serum, the overall assay specificity of the progesterone-3-[125I] RIA is similar to that of the [3H]-progesterone method using dextran-coated charcoal. The results of serum progesterone measurements during the menstrual cycle obtained by the progesterone-3-[125I] RIA appear comparable to those of [3H]-progesterone assays which employ similar anti-progesterone-11 alpha-BSA sera. The progesterone-3-[125I] double-antibody RIA, however, is more convenient and less expensive than the [3H]-progesterone RIA method.
D000888:Antibodies, Anti-Idiotypic; D003429:Cross Reactions; D005260:Female; D006801:Humans; D008024:Ligands; D008598:Menstruation; D010060:Ovulation; D011374:Progesterone / Q000031:analogs & derivatives / Q000097:blood*; D011863:Radioimmunoassay / Q000379:methods; D012710:Serum Albumin, Bovine
D000888:Antibodies, Anti-Idiotypic; D008024:Ligands; D012710:Serum Albumin, Bovine; D011374:Progesterone