"NK-like" cytotoxicity of human lymphocytes cultured in media containing fetal bovine serum.
Human peripheral blood lymphocytes (PBL) were found to be highly cytotoxic to a human malignant melanoma target cell line after the PBL were cultured in medium supplemented with 20% fetal bovine serum (FBS). The effector cells had surface markers similar to those of non-cultured NK effectors against melanoma targets. Both NK (without culture) and the culture-induced cytotoxic effectors had Fc gamma receptors, lacked Fc mu receptors and were either heterogeneous for E receptors or had low-affinity E receptors. Cells required to generate the FBS response were sensitive to anti-T serum plus complement, passed through a nylon-fiber column, formed E rosettes, but lacked detectable receptors for the Fc portions of either IgG or IgM. These results suggest that events causing activation of T cells such as culture of PBL in FBS-containing medium, result in the augmentation of NK activity.
D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity; D002417:Cattle; D002460:Cell Line; D002478:Cells, Cultured; D003470:Culture Media; D003602:Cytotoxicity, Immunologic; D005312:Fetal Blood; D006801:Humans; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007694:Killer Cells, Natural / Q000276:immunology*; D008213:Lymphocyte Activation; D008212:Lymphocyte Depletion; D008214:Lymphocytes / Q000276:immunology*; D011947:Receptors, Antigen, B-Cell; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000276:immunology
D003470:Culture Media; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011947:Receptors, Antigen, B-Cell