title: Lactate dehydrogenase isoenzyme pattern: marker for differentiation of lymphocytes.
abstract: The lactate dehydrogenase isoenzyme pattern has been determined in different murine thymocyte cell populations. Enrichment of thymocytes with more mature cells through total irradiation results in a smaller percentage of LDH-1, LDH-2, LDH-3 and a greater percentage of LDH-4 and LDH-5. Fractionation of normal thymocytes by velocity sedimentation at unit gravity yields a fraction of cells with a high sedimentation rate. LDH-1 and LDH-2 formed a smaller percentage of the total enzyme activity in these cells. These findings indicate that the LDH isoenzyme distribution is a marker for differentiation of thymocytes.
mesh_terms: D000818:Animals; D001799:Blood Sedimentation; D002454:Cell Differentiation; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000097:blood*; D007958:Leukocyte Count; D008297:Male; D051379:Mice; D013601:T-Lymphocytes / Q000201:enzymology*
chemical_list: D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase