title: Activation of electrogenic Na+ pump by epinephrine in bullfrog atrium.
abstract: The effects of epinephrine (1 X 10(-6)--1 X 10(-5) M) on the resting membrane potential and the K+-activated hyperpolarization of bullfrog atrial heart muscles were studied by the single sucrose-gap method or by intracellular microelectrode filled with 3 M KC1. Epinephrine-induced hyperpolarization (Ep-hyperpolarization) was completely eliminated by the effect of ouabain or by removal of extracellular K+. The amplitude of K+-activated hyperpolarization, which was produced when the extracellular K+ concentration was raised from zero to 2 mM, was markedly increased in the presence of epinephrine. The membrane depolarizations, due to high extracellular K+ concentration in the presence of ouabain (5 X 10(-6) M) which completely and reversibly eliminated the K+-activated hyperpolarization, were not altered under the effect of epinephrine. These results suggested that Ep-hyperpolarization of bullfrog atrial heart muscles was due to the acceleration of electrogenic Na+ pump which produced the K+-activated hyperpolarization.
mesh_terms: D000818:Animals; D004837:Epinephrine / Q000494:pharmacology*; D006321:Heart / Q000187:drug effects / Q000502:physiology; D066298:In Vitro Techniques; D008564:Membrane Potentials; D009206:Myocardium / Q000378:metabolism*; D010042:Ouabain / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D011892:Rana catesbeiana; D012964:Sodium / Q000378:metabolism*
chemical_list: D010042:Ouabain; D012964:Sodium; D011188:Potassium; D004837:Epinephrine