title: Interaction of DNA with DNA binding proteins. II. Displacement of Escherichia coli DNA unwinding protein and the condensed structure of DNA complexed with protein HD.
abstract: Three DNA binding proteins from Escherichia coli cells have been complexed with single-stranded phage fd DNA. Electron microscopy reveals granular substructures in the complexes formed with protein HD. In complexes of DNA unwinding protein with fd DNA both protein HD and phage-coded gene 5 protein partially displace the unwinding protein which results in the formation of structures characteristic for the DNA complexes formed with either protein HD or gene 5 protein alone. Combination of protein HD with double-stranded phage T7 DNA leads to a progressive folding and condensing of the genome. The structures observed are discussed in relation to current concepts of the packing of DNA in protein complexes.
mesh_terms: D001426:Bacterial Proteins; D002352:Carrier Proteins; D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D004926:Escherichia coli; D008854:Microscopy, Electron; D014764:Viral Proteins
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D014764:Viral Proteins