title: Specificity of anti-human IgG antibodies in antiglobulin (Coombs) sera.
abstract: The agglutination test with latex particles coated with aggregated human IgG was introduced into the evaluation of Coombs serum as an additional test for anti-IgG antibody activity. In Coombs sera prepared by the conventional immunization method employing Freund's adjuvant, latex agglutination titers were found much lower than those of anti-D-coated red cell agglutination. On the other hand, in sera prepared by other immunization methods, such as the one according to Haynes and Chaplin (1971), anti-IgG antibody response was readily observed by IgG-coated latex agglutination. Specificity of anti-IgG antibodies in the latter sera seems to be predominantly directed to aggregated human IgG.
mesh_terms: D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000032:analysis*; D000918:Antibody Specificity; D003298:Coombs Test; D005620:Freund's Adjuvant; D006801:Humans; D007114:Immunization; D007074:Immunoglobulin G; D007841:Latex Fixation Tests; D011817:Rabbits / Q000276:immunology; D012756:Sheep / Q000276:immunology; D013045:Species Specificity
chemical_list: D000888:Antibodies, Anti-Idiotypic; D007074:Immunoglobulin G; D005620:Freund's Adjuvant