Two-color immunofluorescence using a fluorescence-activated cell sorter.
A technique for the analysis of fluorescein and rhodamine in a flow system using a single wavelength of excitation is described. Both optics and electronics are used to discriminate the fluorescein and rhodamine signals. This technique has been used to study the relationship between immunoglobulin M and immunoglobulin D on mouse splenic lymphocytes.
D002469:Cell Separation / Q000295:instrumentation*; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique / Q000295:instrumentation*; D007072:Immunoglobulin D / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D008214:Lymphocytes / Q000276:immunology*; D012235:Rhodamines; D013154:Spleen / Q000166:cytology
D005452:Fluoresceins; D007072:Immunoglobulin D; D007075:Immunoglobulin M; D012235:Rhodamines