Human granulocyte specific nuclear antigen(s). I: Production of antisera and determination of specificity.
Non-histone protein-DNA complexes isolated from human lung tissue were used to immunize New Zealand rabbits. Quantitative microcomplement fixation and immunocytochemistry revealed a specific chromatin antigen(s) in the human granulocyte nucleus. The study demonstrates the feasibility of employing antisera against chromosomal non-histone protein-DNA complexes for the immunochemical identification of cell types.
D000818:Animals; D000906:Antibodies; D000974:Antibodies, Antinuclear; D000918:Antibody Specificity; D000941:Antigens; D002843:Chromatin / Q000276:immunology*; D002868:Chromosomal Proteins, Non-Histone / Q000276:immunology; D004247:DNA / Q000276:immunology; D006098:Granulocytes / Q000276:immunology*; D006801:Humans; D008168:Lung / Q000276:immunology; D009928:Organ Specificity; D011817:Rabbits
D000906:Antibodies; D000974:Antibodies, Antinuclear; D000941:Antigens; D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D004247:DNA