Localized mutagenesis with bacteriophage Mu: method for increasing the frequency of specific bacterial mutants.
A method is described for markedly enriching a bacterial population for cells containing any given Mu insertion mutation. The method involves the transfer of a small piece of deoxyribonucleic acid from a Mu-infected Hfr donor donor strain to a suitable F- strain and a subsequent selection of those recombinant organisms that have received a Mu prophage from the donor. The method is particularly usefule for isolating mutants whose selection requires "brute-force" assay, since only a few hundred colonies have to be screened.
D003090:Coliphages / Q000235:genetics* / Q000254:growth & development; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D008242:Lysogeny; D008722:Methods; D009154:Mutation; D011995:Recombination, Genetic; D014162:Transfection