Effect of rifampicin on the infectivity of RNA bacteriophage f2.
RNA bacteriophage f2, treated in vitro with rifampicin, loses infectivity dramatically. Rifampicin interacts with phage RNA, binding to a few specific sites. Inhibition of phage RNA infectivity occurs at 10-100 times lower molar excess of rifampicin than inhibition of infectivity of intact phage particles. Thus the phage capsid acts as a barrier, diminishing interaction of the drug with phage RNA.
D003090:Coliphages / Q000187:drug effects / Q000502:physiology*; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000187:drug effects / Q000502:physiology; D007700:Kinetics; D012293:Rifampin / Q000494:pharmacology*; D013053:Spectrophotometry; D013104:Spheroplasts / Q000187:drug effects / Q000502:physiology; D014779:Virus Replication / Q000187:drug effects
D012293:Rifampin