title: The laboratory diagnosis of Nairobi sheep disease.
abstract: The laboratory methods available for the isolation and identification of Nairobi sheep disease virus have been compared. The results show that inoculation of tissue culture (BHK 21 C 13) with suspensions of infected organs or plasma followed by fluorescent antibody tests on coverslip preparations gave the quickest means of identification. This test did not depend on the production of a cytopathic effect. Primary isolation of the virus in infant mouse brain and identification either by fluorescent antibody methods or by complement fixation with antigen prepared from the mouse brain offers a slightly more sensitive isolation system and would be recommended where no tissue culture facility exists.
mesh_terms: D000818:Animals; D001103:Arboviruses / Q000254:growth & development / Q000276:immunology / Q000302:isolation & purification; D002478:Cells, Cultured; D003168:Complement Fixation Tests; D003588:Cytopathogenic Effect, Viral; D005455:Fluorescent Antibody Technique; D051379:Mice; D009265:Nairobi Sheep Disease / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D012756:Sheep