[In vitro cell culture of the Ararat cochineal insect, Porphyrophora hamelii Brandt].
The possibility of cultivating the Ararat cochineal cells in the nutrient media developed for cell cultures of the other insect species was investigated. The initial cochineal cell culture was obtained in suspension. The cell culture on the substrate (glass) dies within 7 days. 2 peaks of proliferative activity was observed in the suspension culture. The increase of cell ploidy and their death were found between these two peaks. The small cells survived and kept their capacity for proliferation. The hemolymph did not stimulate cell proliferation but enabled their better attachment to the substrate. The use of different media did not reveal marked differences in cell behaviour. The pigment granules disappeared during the first week of cultivation.
D000818:Animals; D002455:Cell Division; D002467:Cell Nucleus / Q000648:ultrastructure; D002478:Cells, Cultured; D003470:Culture Media; D003584:Cytological Techniques; D003593:Cytoplasm / Q000648:ultrastructure; D006458:Hemolymph / Q000378:metabolism; D007313:Insecta / Q000166:cytology*; D010860:Pigments, Biological / Q000096:biosynthesis; D013997:Time Factors
D003470:Culture Media; D010860:Pigments, Biological
Kul'tura kletok Araratskoĭ koshenili Porphyrophora hamelii Brandt in vitro.