The use of porous plastic filters in the preparation of cell suspensions.
Suspensions of cells were prepared by dissociation of various tissues with the proteolytic enzyme pronase. Debris, undisrupted tissue and cell clumps were effectively removed from the suspensions by passage through VYON F porous plastic filters whereas isolated cells were recovered without significant losses. The viability of the cells, as judged by dye exclusion and subsequent behavior in tissue culture, was not affected by the treatment.
D000818:Animals; D002470:Cell Survival; D002477:Cells / Q000201:enzymology; D002642:Chick Embryo; D046508:Culture Techniques; D003584:Cytological Techniques; D005374:Filtration; D006168:Guinea Pigs; D051379:Mice; D010969:Plastics; D011402:Pronase / Q000378:metabolism; D013535:Suspensions
D010969:Plastics; D013535:Suspensions; D011402:Pronase