Evidence for incorporation of thymidine into deoxyribonucleic acid in airborne bacterial cells.
As part of an effort to discover whether bacteria might propagate within airborne particles, we studied the incorporation of thymidine into the trichloroacetic acid-insoluble fraction of airborne cells of Serratia marcescens to seek evidence of the possible formation of new DNA. Two aerosols, one of S. marcescens and another of [3H]thymidine ([3H]dT) suspended in growth medium were caused to aggregate in air just prior to directing the aerosols into rotating-drum aerosol storage chambers. The age of the S. marcescens culture and other conditions for maximizing ([3H]dT) uptake were selected on the basis of prior in vitro trials. With 10-h cultures and addition of 2-deoxyadenosine to the [3H]dT, we showed that [3H]dT is incorporated into the trichloroacetic acid-insoluble fraction of cells recovered 6 h after aerosols were stored under the conditions of high humidity and 30 degrees C. Tests conducted in the same manner with Formalin-killed S. marcescens ruled out the possibility of adsorptive carry-over of [3H]dT. As much as 20 times more activity was found in the trichloroacetic acid-insoluble fraction of live cells than of dead cells.
D000336:Aerosols; D000391:Air Microbiology; D002455:Cell Division; D004269:DNA, Bacterial / Q000096:biosynthesis*; D005557:Formaldehyde / Q000494:pharmacology; D012706:Serratia marcescens / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D013936:Thymidine / Q000378:metabolism*
D000336:Aerosols; D004269:DNA, Bacterial; D005557:Formaldehyde; D013936:Thymidine