[Study of the antibody-dependent lymphocytotoxicity test as a function of the nature of the antibodies].
It is well known that IgM antibodies are inactive for the LDA test. Moreover, this work showed 7 S IgM to be just as inactive as 19 S IgM. With HLA antibodies, usually IgG, this work showed that the LDA test was not inhibited by a temperature of + 15 instead of + 37 degrees C. With cold lymphocytotoxic antibodies 19 S and 7 S IgM the LDA test was negative even at + 15 degrees C (optimum temperature for these complement dependant lymphocytotoxic antibodies) with or without prolonged incubation. Under these same conditions, a negative result was obtained using auto-lymphocytes as a target. Finally, our results confirmed that IgM antibodies with anti-HLA activity could not act as mediators even for targets with corresponding HLA activity.
D000961:Antilymphocyte Serum; D003601:Cytotoxicity Tests, Immunologic; D005260:Female; D006680:HLA Antigens; D006649:Histocompatibility Antigens; D006650:Histocompatibility Testing; D006801:Humans; D007075:Immunoglobulin M; D008214:Lymphocytes / Q000276:immunology*; D008233:Lymphotoxin-alpha; D011247:Pregnancy; D013696:Temperature
D000961:Antilymphocyte Serum; D006680:HLA Antigens; D006649:Histocompatibility Antigens; D007075:Immunoglobulin M; D008233:Lymphotoxin-alpha
Etude du test de lymphocytotoxicité anticorps-dépendante en fonction de la nature de l'anticorps