Methods for producing T- and B-lymphocyte receptor-specific antisera.
Spent tissue culture medium from two continuous lymphoblastoid cell lines, FL-74 and CT45-S, expressing the T-lymphocyte receptor for guinea pig E and the B-lymphocyte receptor for EAC respectively were used to produce receptor-specific antisera. Anti-E receptor sera blocked E rosette formation on FL-74 cells, canine and feline lymphocytes and canine and feline thymocytes but not EAC rosette formation by CT45-S cells or canine and feline lymphocytes. Anti-EAC receptor sera blocked EAC rosette formation on CT45-S cells and canine or feline lymphocytes. Absorption of antisera will the appropriate lymphoblastoid cell line removed E or EAC-blocking activity. The results of this study suggest that similar methods may be used to produce lymphocyte subpopulation-specific antisera in other species including man.
D000818:Animals; D000918:Antibody Specificity; D000961:Antilymphocyte Serum / Q000302:isolation & purification*; D001402:B-Lymphocytes / Q000276:immunology*; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D002415:Cats; D004285:Dogs; D006168:Guinea Pigs; D006801:Humans; D007158:Immunologic Techniques; D013601:T-Lymphocytes / Q000276:immunology*
D000961:Antilymphocyte Serum