[Immunofluorescence studies of experimental glomerulonephritis in formalin-fixed and paraffin-embedded material (author's transl)].
Immunofluorescence studies are an essential part of the diagnosis in glomerulonephritis. For these studies unfixed, frozen material was used only, on account of intense background staining of formalin-fixed slides. A method developed by Huang (1975) was tested on experimental glomerulonephritis. He succeeded in minimizing the background staining of formalin-fixed liver tissue with pronase-treatment. Our results show no differences in the immunofluorescence of the experimental glomerulonephritis between frozen and paraffin-embedded sections. It seems to be possible therefore to investigate a glomerulonephritis in formalin-fixed material by this method.
D000818:Animals; D005455:Fluorescent Antibody Technique; D005629:Frozen Sections; D005921:Glomerulonephritis / Q000378:metabolism*; D006652:Histological Techniques; D007668:Kidney / Q000032:analysis*; D008297:Male; D051379:Mice; D010232:Paraffin; D011402:Pronase; D013048:Specimen Handling
D010232:Paraffin; D011402:Pronase
Immunfluoreszenzoptische Untersuchungen zur experimentellen Glomerulonephritis an Formalin-fixiertem und Paraffin-eingebettetem Gewebe.