Production and assay of antibodies against one antigenic component of Mycobacterium bovis BCG.
Monospecific antisera against BCG antigen 60 were produced by all of four rabbits during immunization with precipitates containing antigen 60 cut out of gels after crossed immunoelectrophoresis. During electrolytic iodination of a crude antigen 60 preparation, preferential labeling of antigen 60 was demonstrated, and a specific radioimmunoassay was established to follow the development of anti-BCG-60 activity during immunization.
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D000942:Antigens, Bacterial / Q000032:analysis / Q000302:isolation & purification; D001500:BCG Vaccine; D003378:Counterimmunoelectrophoresis; D007106:Immune Sera / Q000494:pharmacology; D009163:Mycobacterium bovis / Q000276:immunology*; D011817:Rabbits; D011863:Radioimmunoassay
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001500:BCG Vaccine; D007106:Immune Sera