title: Physicochemical consequences of opsonization: perturbation of liposomal membranes by Salmonella typhimurium 395 MS opsonized with IgG antibodies.
abstract: When phagocytosis-resistant Salmonella typhimurium 395 MS bacteria sensitized with anti-MS IgG antibodies were incubated with liposomes composed of phosphatidylcholine, cholesterol, and dicetylphosphate, a previously sequestered liposomal marker (4-methylumbelliferylphosphate) was released. Unsensitized or F(ab')2-sensitized bacteria had no such effect. This perturbation was neither dependent upon negatively charged dicetylphosphate nor upon cholesterol. It was further evident that palmitoyl-poly(ethyleneglycol) caused release of the trapped marker in a similar way as  sensitized bacteria. These findings demonstrate a similarity between sensitized bacteria and a hydrophobic probe and lend support to the hypothesis that the perturbation was brought about by hydrophobic interaction. The observations indicate that liposomes, like phagocytes, possess "receptor sites" for the activated part of IgG and raise the possibility that phagocytic effectors can operate in a relatively nonspecific manner.
mesh_terms: D000907:Antibodies, Bacterial / Q000008:administration & dosage*; D001665:Binding Sites; D002784:Cholesterol / Q000276:immunology; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D066298:In Vitro Techniques; D008081:Liposomes / Q000276:immunology*; D008564:Membrane Potentials; D008566:Membranes / Q000276:immunology; D009895:Opsonin Proteins; D009943:Organophosphorus Compounds / Q000276:immunology; D010713:Phosphatidylcholines / Q000276:immunology; D012486:Salmonella typhimurium / Q000276:immunology*; D014468:Umbelliferones / Q000276:immunology
chemical_list: D000907:Antibodies, Bacterial; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D008081:Liposomes; D009895:Opsonin Proteins; D009943:Organophosphorus Compounds; D010713:Phosphatidylcholines; D014468:Umbelliferones; D002784:Cholesterol