Effect of 1.25-dihydroxycholecalciferol administration on blood glucose and pancreatic islet morphology in mice.
1.25-dihydroxycholecalciferol (DHCC) administration to fed and starved mice had no effect on the blood-glucose concentration or the light-microscopic appearance of the endocrine or exocrine pancreas. Electron microscopy, however, disclosed changes which appeared early after the injection and were more marked in starved than in fed animals. The B-cells exhibited mitochondrial hypertrophy, studied both by qualitative and quantitative methods, invagination and vacuolation of mitochondrial membranes, increased occurrence of light secretory granules, multiple rough endoplasmic cisternae, multi-lamellar bodies, and a rather rich Ca2+-containing pyroantimonate precipitation mainly localized to nuclei and mitochondria. A tendency to mitochondrial hypertrophy was observed also in some D-cells. The A-cells were unaffected. The findings indicate that the endocrine pancreas (or at least the B-cells) is affected in some way, directly or indirectly, by DHCC.
D000818:Animals; D001786:Blood Glucose / Q000032:analysis*; D002467:Cell Nucleus / Q000648:ultrastructure; D003593:Cytoplasm / Q000648:ultrastructure; D004100:Dihydroxycholecalciferols / Q000494:pharmacology*; D005260:Female; D006887:Hydroxycholecalciferols / Q000494:pharmacology*; D007515:Islets of Langerhans / Q000187:drug effects* / Q000473:pathology / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008854:Microscopy, Electron; D008928:Mitochondria / Q000473:pathology / Q000648:ultrastructure; D013997:Time Factors
D001786:Blood Glucose; D004100:Dihydroxycholecalciferols; D006887:Hydroxycholecalciferols