Reproducibility of interpretation of the test for antibody-coated bacteria in urinary sediment.
The direct immunofluorescence technique for detecting antibody-coated bacteria in urinary sediment is felt to be useful in distinguishing infection of the kidney from infection of the bladder. An independent, blind multiple-reading system was used to measure interobserver variability in the evaluation of slides of urinary sediments for antibody-coated bacteria. Three independent observers agreed unanimously on first reading in 88% of 253 specimens. When compared with the majority opinion, the sensitivity and specificity of an individual reading were 91 and 95%, respectively.
D001419:Bacteria / Q000276:immunology / Q000302:isolation & purification*; D001666:Binding Sites, Antibody; D003937:Diagnosis, Differential; D005069:Evaluation Studies as Topic; D005455:Fluorescent Antibody Technique; D006801:Humans; D007674:Kidney Diseases / Q000175:diagnosis*; D001745:Urinary Bladder Diseases / Q000175:diagnosis*; D014552:Urinary Tract Infections / Q000175:diagnosis*; D014556:Urine / Q000382:microbiology*