Detection of low avidity anti-DNA antibodies in systemic lupus erythematosus.
The binding of sonicated, radiolabeled DNA by systemic lupus erythematosus (SLE) sera was measured by a high sensitivity polyethylene glycol (PEG) precipitation assay. This method is considerably more sensitive than currently used techniques. The results suggest that a significant concentration of low avidity antibodies is present in SLE sera; however, these antibodies are not detected by conventional techniques.
D000974:Antibodies, Antinuclear / Q000032:analysis*; D000915:Antibody Affinity; D000936:Antigen-Antibody Complex; D011232:Chemical Precipitation; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D008722:Methods; D011092:Polyethylene Glycols; D014316:Tritium; D014462:Ultrafiltration
D000974:Antibodies, Antinuclear; D000936:Antigen-Antibody Complex; D014316:Tritium; D011092:Polyethylene Glycols