title: Differential effect of hydrocortisone on lymphocyte populations in the mouse spleen.
abstract: The cellular composition of the spleen from CBA mice was investigated two days after administration of increasing doses of hydrocortisone acetate (HC), this using the presence of surface-immunoglobulin and the electrophoretic mobility as criteria for identification of lymphocyte populations. The B cell content was found to decrease more rapidly than did the T cell content. However, a significant diminution of the modal volume of both B and T cells was observed which suggests that HC acted similarly on each of these lymphocyte compartments by removing the larger elements. On the other hand, the in vitro reactivities to the B cell mitogens LPS and PPD were strongly depressed by HC injection while the reactivities to the T cell mitogens Con A and PHA remained relatively unchanged. Therefore, HC treatment appears to affect both B and T lymphocyte populations in the mouse spleen but the former to a greater extent than the latter.
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000187:drug effects*; D003208:Concanavalin A / Q000494:pharmacology; D006854:Hydrocortisone / Q000494:pharmacology*; D037102:Lectins / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D013154:Spleen / Q000166:cytology*; D013601:T-Lymphocytes / Q000187:drug effects*; D014373:Tuberculin / Q000494:pharmacology
chemical_list: D037102:Lectins; D008070:Lipopolysaccharides; D011947:Receptors, Antigen, B-Cell; D014373:Tuberculin; D003208:Concanavalin A; D006854:Hydrocortisone