Leukocyte inhibitory factor (LIF) production from lymphocytes stimulated by leucogenenol.
It has been demonstrated that human peripheral lymphocytes incubated in a tissue culture medium containing 0.04, 0.1, or 0.15 microgram/ml of leucogenenol form or release a factor that inhibits the migration of human peripheral PMN leukocyte. The factor is chromatographed of Sephadex G-100 and migrates on electrophoresis as an albumin, thus suggesting that it has the electric charge of an albumin. The factor is stable to neuraminidase and to heating at 56 degrees C for 30 min, but it is inactivated by heating at 80 degrees C for 60 min. Its physical and biological properties suggest that the factor is identical to the LIF reported by Rocklin.
D002464:Cell Migration Inhibition; D002478:Cells, Cultured; D006801:Humans; D007158:Immunologic Techniques; D066298:In Vitro Techniques; D007932:Leucogenenol / Q000494:pharmacology*; D007957:Leukocyte Adherence Inhibition Test; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D008263:Macrophage Migration-Inhibitory Factors / Q000096:biosynthesis*; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism; D013141:Spiro Compounds / Q000494:pharmacology*
D008263:Macrophage Migration-Inhibitory Factors; D013141:Spiro Compounds; D007932:Leucogenenol