Glutamine as a feedback inhibitor of the Rhodopseudomonas sphaeroides nitrogenase system.
In whole cells of Rhodopseudomonas sphaeroides, nitrogen fixation, as measured by hydrogen production and acetylene reduction, was totally inhibited by micromolar concentrations of ammonia. This inhibition could not be duplicated by glutamate or glutamine alone. The inhibition by ammonia was abolished by methionine sulfoximine, a glutamine synthetase inhibitor. Inhibition by glutamine was complete in the presence of methionine sulfone, a preferential inhibitor of glutamate synthase, presumably by permitting a rise in the glutamine pool. The results indicated that the level of the glutamine pool controlled the activity of nitrogenase. None of these effects could be duplicated with cell-free nitrogenase, indicating there is probably a mediator which responds to the glutamine pool and inhibits nitrogenase, rather than glutamine itself being a direct inhibitor.
D000641:Ammonia / Q000494:pharmacology; D005246:Feedback; D005973:Glutamine / Q000378:metabolism* / Q000494:pharmacology; D007700:Kinetics; D008717:Methionine Sulfoximine / Q000494:pharmacology; D009591:Nitrogenase / Q000378:metabolism*; D012242:Rhodobacter sphaeroides / Q000187:drug effects / Q000201:enzymology*
D005973:Glutamine; D008717:Methionine Sulfoximine; D000641:Ammonia; D009591:Nitrogenase