title: Pre-early polypeptides of bacteriophages T5 and BF23.
abstract: Nine pre-early polypeptides have been detected after infection with bacteriophage T5 and 10 pre-early polypeptides have been detected after infection with bacteriophage BF23. Only about one-half of the coding capacity of the redundant ends of the phage DNA, which code for pre-early proteins, is needed for these 9 to 10 pre-early polypeptides. The direction of transcription of pre-early genes A1 and A2 has been established from the size of N-terminal polypeptide fragments induced by amber mutants and from the known intragenic loci of the amber mutations. Some pre-early functions appear to be nonessential, because a viable deletion mutant of BF23 fails to induce three and possibly four of the detectable pre-early polypeptides.
mesh_terms: D003090:Coliphages / Q000032:analysis* / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D005796:Genes; D008970:Molecular Weight; D009154:Mutation; D010455:Peptides / Q000032:analysis*; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000032:analysis*
chemical_list: D004279:DNA, Viral; D010455:Peptides; D014764:Viral Proteins