Affinity chromatography od Klebsiella arylsulfatase on tyrosyl-hexamethylenediamine-beta-1,3-glucan and immunoadsorbent.
A simple and convenient method for preparation of a highly purified arylsulfatase (EC 3.1.6.1) from Klebsiella aerogenes has been developed. Specificity of purification was achieved by using affinity chromatography on a tyrosyl-hexamethylenediamino-beta-1,3-glucan or on a solid phase immunoadsorbent. By using affinity chromatography a homogeneous enzyme was obtained with high yield. It is also proposed that the beads of curdlan type polysaccharide consisting of beta-1,3-glucan can be used as a good matrix for affinity chromatography.
D001192:Arylsulfatases / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods; D007164:Immunosorbents; D007711:Klebsiella pneumoniae / Q000201:enzymology*; D011135:Polysaccharides, Bacterial; D013429:Sulfatases / Q000302:isolation & purification*; D014443:Tyrosine / Q000031:analogs & derivatives
D007164:Immunosorbents; D011135:Polysaccharides, Bacterial; D014443:Tyrosine; D013429:Sulfatases; D001192:Arylsulfatases