Surface immunoglobulin phenotype of murine spleen cells which form B cell colonies in agar.
Separation of IgD-bearing B lymphocytes from the spleens of 3-week-old or adult mice has been achieved using cellular affinity columns and an anti-delta allotype serum. The results demonstrate that the majority of cells capable of forming B lymphocyte colonies in agar are delta-positive although they lose detectable IgD upon stimulation. It is proposed that these cells are the descendants of IgD-negative cells found in the fetal liver and neonatal spleen.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D002462:Cell Membrane / Q000276:immunology; D002846:Chromatography, Affinity; D007072:Immunoglobulin D / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D010641:Phenotype; D011947:Receptors, Antigen, B-Cell / Q000235:genetics*; D013154:Spleen / Q000276:immunology*
D007072:Immunoglobulin D; D011947:Receptors, Antigen, B-Cell