In vivo generation and regulation of killer T-cells directed towards hapten-coupled syngeneic cells.
We have extended our studies which show that hapten-specific killer T-cells can be generated in vivo towards hapten-conjugated, syngeneic spleen cells provided an auxiliary antigenic stimulus is incorporated. M1s antigen served in this auxiliary capacity by stimulating helper T-cells. Natural or experimental tolerance to these antigens abrogated cytotoxicity. Effecting tolerance in host animals towards target hapten also prevented development of the genetically restricted killer T-cells. Thus, cytolytic T-cells can be controlled equally at the levels of helper and pre-killer cells. While adoptive transfer of hapten-specific tolerance by means of spleen cells did not abrogate appearance of hapten-specific killer T-cells, it did prevent hapten-specific DTH. Thus, the mechanism for regulating the latter may differ significantly from that for CML.
D000818:Animals; D003602:Cytotoxicity, Immunologic; D005260:Female; D006241:Haptens; D006649:Histocompatibility Antigens; D006968:Hypersensitivity, Delayed / Q000517:prevention & control; D007108:Immune Tolerance; D007116:Immunization, Passive; D051379:Mice; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014301:Trinitrobenzenes / Q000276:immunology
D006241:Haptens; D006649:Histocompatibility Antigens; D014301:Trinitrobenzenes