Nocardia water-soluble mitogen and lipopolysaccharide. Comparative study of two adjuvants and B-cell mitogens in mice.
The activity of Nocardia water-soluble mitogen (NWSM) and LPS were compared in several experimental systems, since both compounds are B-cell mitogens and polyclonal activators in vitro. The results reported here demonstrated that NWSM like LPS also has a strong adjuvant activity in vivo if administered in saline with a strong antigen (heterologous red blood cells) or even with a weak immunogen such as theta alloantigen. However, in contrast to LPS, NWSM administered to mice failed to induce in vivo proliferation of lymphocytes, polyclonal activation and PFC against syngeneic bromelain-treated erythrocytes and thymocytes. It is possible therefore, that different mechanisms may be responsible for adjuvant activity of NWSM and LPS.
D000276:Adjuvants, Immunologic; D000818:Animals; D000921:Antibody-Producing Cells; D001963:Bromelains; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D006462:Hemolytic Plaque Technique; D007518:Isoantibodies / Q000032:analysis*; D007519:Isoantigens; D008070:Lipopolysaccharides / Q000276:immunology*; D051379:Mice; D008934:Mitogens; D009615:Nocardia / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
D000276:Adjuvants, Immunologic; D007518:Isoantibodies; D007519:Isoantigens; D008070:Lipopolysaccharides; D008934:Mitogens; D001963:Bromelains