Cellular cooperation in lymphocyte activation. III. B-cell helper effect in the enhancement of T-cell response.
T and B cells were purified from human tonsil and peripheral blood by the removal of phagocytic cells, followed by filtration through a nylon fiber column (NC) and E-rosette formation. Purified T and B cells contained less than 1% of other cell types. The responses of T cells to concanavalin A (Con A) and soluble protein A were greatly enhanced in the presence of autologous B cells. Participation of B cells in T-cell enhancement was confirmed by the following observations: (a) purified B copulation, which was separated further from adherent B cells, retained its enhancing activity. (b) Another adherent cell-free B-cell preparation, which was purified from the NC-passed fraction, and (c) no T lymphoid but some B lymphoid cell lines, elicited strong T-cell enhancement. It was also found that the enhancing capacity of B cells required no metabolic activity, but rather an intact cell form and direct cell-to-cell contact with responding cells. The stimulatory determinants on B cells were resistant to trypsin and neuraminidase treatment. In this paper a hypothesis will be presented that at least two signals are prerequisite for the effective activation of T cells.
D001402:B-Lymphocytes / Q000276:immunology*; D002450:Cell Communication; D002460:Cell Line; D003208:Concanavalin A; D006801:Humans; D008213:Lymphocyte Activation; D009439:Neuraminidase / Q000494:pharmacology; D011691:Puromycin / Q000494:pharmacology; D013601:T-Lymphocytes / Q000276:immunology*; D014357:Trypsin / Q000494:pharmacology
D003208:Concanavalin A; D011691:Puromycin; D009439:Neuraminidase; D014357:Trypsin