Effects of ATP and ADP on hydrogen-deuterium exchange in heavy meromyosin. An examination of difference spectra.
The exchange reaction of peptide hydrogens with deuterium has been followed by measuring the decrease of the amide II band for heavy meromyosin (HMM). The difference spectra between HMM and HMM + ATP, between HMM and HMM + ADP, and between HMM + ATP and HMM + ADP have been examined as functions of time in order to detect small differences in the kinetic behavior of these different states of HMM. It has been found that, at 14 degrees C and 26 degrees C (pH 8.0), the exchange reaction is slightly slower for HMM + ATP than for HMM, and slightly slower for HMM + ADP than for HMM + ATP. This indicates that the secondary structure of HMM changes its flexibility during the ATP splitting cycle.
D000244:Adenosine Diphosphate / Q000494:pharmacology*; D000255:Adenosine Triphosphate / Q000494:pharmacology*; D000818:Animals; D003903:Deuterium; D006859:Hydrogen / Q000378:metabolism*; D007700:Kinetics; D015879:Myosin Subfragments / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism; D011817:Rabbits; D013053:Spectrophotometry; D013696:Temperature
D015879:Myosin Subfragments; D010710:Phosphates; D000244:Adenosine Diphosphate; D006859:Hydrogen; D000255:Adenosine Triphosphate; D003903:Deuterium