Stimulation of thymocytes in vitro by serum of 2-acetylaminofluorene (AAF)-treated rats.
The serum of rats obtained at various time intervals after administration of AAF was tested for its action on the 3H-thymidine incorporation into the DNA of thymocytes in vitro. In comparison with untreated or solvent-treated controls, the sera of animals in the early phase after the AAF-treatment stimulate the incorporation of the nucleotide into the thymocytes of rats, mice and hamsters. This reaction shows an unequivocal dose-response relationship. The serum activity is dialysable and relatively thermostable. Spleen lymphocytes and peritoneal cells of rats are insensitve to the AAF-serum. A possible relationship between growth-stimulating serum activities in liver regeneration and in early carcinogen action is discussed.
D015073:2-Acetylaminofluorene / Q000494:pharmacology*; D000818:Animals; D004247:DNA / Q000096:biosynthesis; D006133:Growth Substances / Q000097:blood*; D008213:Lymphocyte Activation; D008297:Male; D051381:Rats; D013601:T-Lymphocytes / Q000378:metabolism*; D013997:Time Factors
D006133:Growth Substances; D004247:DNA; D015073:2-Acetylaminofluorene