Lutropin stimulation of RNA synthesis in corpus luteum chromatin.
Lutropin and human choriogonadotropin stimulated the endogenous chromatin-associated polymerase activity in purified chromatin prepared from nuclei of bovine corpus luteum. Chromatin was incubated in two different buffer systems: one that mainly supports the activity of polymerase I, another that supports the activity of polymerase II and is largely alpha-amanitin sensitive. The hormones lutropin and chorigonadotropin stimulated an increase in the rate of incorporation of [14C]ATP or [14C]UTP into RNA in both buffer systems. Follitropin, prolactin and beta-corticotropin had no stimulatory effect. Neither the alpha nor beta subunit of lutropin stimulated RNA synthesis. When premixed, the subunits rapidly formed the active molecule. A maximum response to RNA synthesis was achieved by a 10(-9) M concentration of human choriogonadotropin. Considerable activity was obtained at 10(-11) M human choriogonadotropin. There was no lutropin stimulation to RNA synthesis using calf thymus DNA and Escherichia coli RNA polymerase.
D000818:Animals; D002417:Cattle; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D002843:Chromatin / Q000187:drug effects / Q000378:metabolism; D003338:Corpus Luteum / Q000187:drug effects / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000201:enzymology; D004971:Estrus; D005260:Female; D007986:Luteinizing Hormone / Q000494:pharmacology*; D046911:Macromolecular Substances; D011247:Pregnancy; D012313:RNA / Q000096:biosynthesis*; D012318:RNA Polymerase I / Q000378:metabolism
D006063:Chorionic Gonadotropin; D002843:Chromatin; D046911:Macromolecular Substances; D012313:RNA; D007986:Luteinizing Hormone; D004247:DNA; D012318:RNA Polymerase I