title: Occurrence of antibodies to single-stranded DNA in ANA negative patients.
abstract: Five patients with clinical features of a connective tissue disease most suggestive of systemic lupus erythematosus were found to have a negative ANA by conventional screening at 1/20 dilution on mouse liver. However, significant titres of antibodies to single-stranded DNA were detected using a double antibody radioimmunoassay. Thus, certain patients have antibodies to DNA restricted to single-stranded determinants as detected by radioimmunoassay with single-stranded DNA as antigen and a negative ANA test.
mesh_terms: D000906:Antibodies / Q000032:analysis*; D000974:Antibodies, Antinuclear / Q000032:analysis*; D004277:DNA, Single-Stranded / Q000276:immunology*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D008180:Lupus Erythematosus, Systemic / Q000276:immunology*; D011863:Radioimmunoassay
chemical_list: D000906:Antibodies; D000974:Antibodies, Antinuclear; D004277:DNA, Single-Stranded; D007074:Immunoglobulin G