Predominance of granulocytopoiesis in bone marrow grafts in the omenta of mice treated with erythropoietin.
The effects of erythropoietin on the differentiation of murine bone marrow injected into the omenta of x-irradiated mice were investigated. Experimental hosts were injected with 2.5 units of erythropoietin on days 0-7 and sacrificed on day 10. Control hosts were injected with saline or sheep serum. After 10 days the grafts were greater than 95% granulocytic regardless of host treatment. Since these grafts contain multipotent hematopoietic stem cells and the experimental hosts were exposed to large doses of erythropoietin, the results of this experiment indicate that a specialized microenvironment is required for murine erythropoiesis in vivo.
D000818:Animals; D001853:Bone Marrow / Q000187:drug effects; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D004920:Erythropoiesis / Q000187:drug effects*; D004921:Erythropoietin / Q000494:pharmacology*; D006098:Granulocytes / Q000187:drug effects / Q000502:physiology*; D007962:Leukocytes / Q000502:physiology*; D008297:Male; D051379:Mice; D009852:Omentum / Q000601:surgery; D013997:Time Factors; D014184:Transplantation, Homologous
D004921:Erythropoietin