title: Comparative inactivation of yeast fatty acid synthetase component enzymes by 100 atmospheres of oxygen.
abstract: Yeast fatty acid synthetase at 4 degrees C was stable during 1- and 2-h exposures to oxygen at 100 atm, but was 48% and 90% inactivated after 20 h and 40 h, respectively, with fatty acid synthesis measured by both radioactive and optical assays. Incubation with dithiothreitol did not restore activity. Component enzyme activities were compared before and after 40 h in 100 atm of oxygen. Ketoacyl reductase activity was most reduced followed by ketoacyl synthetase and then acetyl transferase while malonyl transferase, enoyl reductase and palmitoyl transferase were not significantly inactivated.
mesh_terms: D004355:Drug Stability; D064429:Fatty Acid Synthases / Q000378:metabolism*; D010100:Oxygen / Q000494:pharmacology*; D011312:Pressure; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000201:enzymology*; D013997:Time Factors
chemical_list: D064429:Fatty Acid Synthases; D010100:Oxygen