Detection of anti-lymphocyte antibodies using the immunoperoxidase antiglobulin technic.
Anti-lymphocyte antibodies in sera of multitransfused dialysis patients can be readily identified using the indirect immunoperoxidase method. Concordance between the microlymphocytotoxicity and immunoperoxidase methods was obtained in 61/66 paired specimens. The sensitivity of the immunoperoxidase antiglobulin technic is approximately that of the microlymphocytotoxicity method. This test may be used for lymphocyte crossmatching prior to renal transplantation.
D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D001323:Autoantibodies / Q000032:analysis*; D001667:Binding, Competitive; D003601:Cytotoxicity Tests, Immunologic; D006801:Humans; D007124:Immunoenzyme Techniques; D008214:Lymphocytes / Q000276:immunology*; D006435:Renal Dialysis
D001323:Autoantibodies