A continuous-flow enzyme detector for liquid chromatography.
A detection system has been developed for the selective and sensitive detection of enzymes eluting from a liquid chromatographic column. This system monitors a reaction that the enzyme catalyzes and provides a chemical amplification ranging from 10(4) to 10(5). The detection system consists of a reagent or substrate pump, a post-column reactor packed with non-porous spherical glass beads, and a photometric detector. A linear and selective response to a series of enzymes of clinical importance is demonstrated.
D000429:Alcohol Oxidoreductases / Q000032:analysis; D000469:Alkaline Phosphatase / Q000032:analysis; D001322:Autoanalysis; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D004228:Dithionitrobenzoic Acid / Q000494:pharmacology; D004798:Enzymes / Q000032:analysis*; D007202:Indicators and Reagents; D007700:Kinetics; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008433:Mathematics; D008832:Microchemistry; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D013056:Spectrophotometry, Ultraviolet; D014357:Trypsin / Q000032:analysis
D004798:Enzymes; D007202:Indicators and Reagents; D004228:Dithionitrobenzoic Acid; D000429:Alcohol Oxidoreductases; D007770:L-Lactate Dehydrogenase; D000469:Alkaline Phosphatase; D014357:Trypsin