[Localization by paper blotting technique of electrophoretic fractions on starch gel. Application to alpha 1 - antitrypsin (author's transl)].
The authors describe a paper blotting technique to reveal starch electrophoresis. After 3 minutes of contact between the paper band and the starch gel the former can be either dried during 5' at 90 degrees C then stained with nigrosine or sliced and revealed by an immunserum. This quick technique has been successfully applied to alpha-1-antitrypsin; on the one side phenotypes can be identified 24 hours earlier than with staining method using amido black 10 B and on the other side quality control of the fractionning can be done before the second stage of the Laurell type two-dimension separation.
D004592:Electrophoresis, Starch Gel / Q000379:methods*; D006801:Humans; D010209:Paper; D000515:alpha 1-Antitrypsin / Q000032:analysis*
D000515:alpha 1-Antitrypsin
Repérage par empreinte des fractions electrophorétiques obtenues en gel d'amidon: application à l'alpha-1-antitrypsine.