The interaction of an anti-tumour platinum complex with DNA.
The interaction of the anti-tumour active cis platinum (II) complexes with DNA has been investigated using dichloro(ethylenediamine)platinum(II) and E. coli DNA. Equilibrium dialysis studies indicate that Pt(en)Cl2 binds reversibly to DNA to a saturation value of 0.57 Pt: P, which is consistent with the platinum being bound both monofunctionally and bifunctionally. Pt(en)Cl2 inhibits the intercalation of 9-aminoacridine (9AA) by cross-linking the bases of the double helix, but at no stage does all the bound platinum cross-link. It is suggested that this inhibition of intercalation is due to intrastrand cross-linking.
D000166:Acridines; D001667:Binding, Competitive; D055598:Chemical Phenomena; D002621:Chemistry; D004247:DNA; D004926:Escherichia coli; D009942:Organometallic Compounds / Q000032:analysis; D010984:Platinum / Q000032:analysis
D000166:Acridines; D009942:Organometallic Compounds; D010984:Platinum; D004247:DNA