Effect of high-dose methotrexate with citrovorum factor on human granulopoiesis.
The present studies were performed to delineate the effect of high-dose methotrexate with citrovorum factor rescue on the measurable compartments of human granulopoiesis, including peripheral blood cells and bone marrow stem cells committed to granulopoiesis-monocytopoiesis. Parameters of granulopoiesis were determined during the first course of high-dose methotrexate with citrovorum factor rescue in five patients, and during four consecutive courses in one patient. The remarkably mild toxicity of high-dose methotrexate with citrovorum factor rescue reported in clinical studies was substantiated on the level of the committed stem cell compartment. The data suggest a higher sensitivity of in vitro colony-forming units in agar culture as compared to the more mature granulopoietic cells and document the exquisite sensitivity and regenerating capacity of erythroid precursors after this type of chemotherapy.
D001853:Bone Marrow / Q000473:pathology; D003114:Colony-Forming Units Assay; D004359:Drug Therapy, Combination; D006098:Granulocytes / Q000187:drug effects*; D006410:Hematopoiesis / Q000187:drug effects*; D006801:Humans; D002955:Leucovorin / Q000008:administration & dosage / Q000009:adverse effects*; D008727:Methotrexate / Q000008:administration & dosage / Q000009:adverse effects* / Q000097:blood; D012516:Osteosarcoma / Q000188:drug therapy
D002955:Leucovorin; D008727:Methotrexate