Use of the avidin-biotin complex for the localization of actin and myosin with fluorescence microscopy.
A new indirect method for fluorescence localization of proteins making use of the avidin-biotin complex is described. We have prepared both a biotin-modified rabbit heavy meromyosin (BHMM) and a biotin-modified antibody to a smooth muscle myosin. After fixation, cells can be treated with either BHMM, which binds to actin, or the biotinyl antibody, which binds to myosin. In a second step the cell are treated with a fluorescent derivative of avidin (Fl-avidin) which binds to the biotinyl proteins and thus indirectly reveals the location of the cellular action or myosin.
D000199:Actins / Q000302:isolation & purification*; D001360:Avidin; D001710:Biotin; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D007074:Immunoglobulin G; D015879:Myosin Subfragments; D009218:Myosins / Q000302:isolation & purification*
D000199:Actins; D007074:Immunoglobulin G; D015879:Myosin Subfragments; D001360:Avidin; D001710:Biotin; D009218:Myosins