Mechanisms of resistance to infection with Coccidioides immitis in mice.
Serum from vaccinated mice was ineffective in neutralizing the infectivity of arthrospores of Coccidioides immitis for recipient mice. However, a T-cell-enriched lymphocyte population was effective in preventing a lethal infection. Spleen cells from immune mice were passaged through nylon wool columns resulting in a T-cell enriched, B-cell-depleted population as shown by the susceptibility of the cell population to anti-theta serum and the inability of the cells to transfer adoptively an immune response to ovalbumin. Whereas transfer of 5 x 10(7) unfractionated immune spleen cells was required to protect 100% of the recipients against a lethal infection with C. immitis, 7 x 10(6) T-cell-enriched immune spleen cells were sufficient for the same level of protection. Thus, transfer of resistance to infection was achieved with fewer cells after the removal of B cells from the transferred spleen cells. The results confirm that T cells are crucial in transferring resistance against infection with C. immitis in mice.
D000818:Animals; D000908:Antibodies, Fungal; D001402:B-Lymphocytes / Q000276:immunology; D003045:Coccidioides / Q000276:immunology; D003047:Coccidioidomycosis / Q000276:immunology*; D005260:Female; D007109:Immunity; D007111:Immunity, Cellular; D007116:Immunization, Passive; D051379:Mice; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D000908:Antibodies, Fungal