[Separation of lymphocytes into classes from peripheral human blood using centrifugation on a discontinuous gradient].
Peripheral blood lymphocytes of 20 normal volunteers were separated on a discontinuous gradient of Ficoll. The ability of lymphocytes from the fractions to from sponteneous (E) and induced (EAC) rosettes and undergo transformation in the presence of phytohaemagglutinin (PHA) was tested. T-lymphocytes were concentrated in the density of 1,067 g/ml and B-lymphocytes in the density of 1,063 g/ml.
D001402:B-Lymphocytes; D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient; D006801:Humans; D008213:Lymphocyte Activation; D010835:Phytohemagglutinins / Q000494:pharmacology; D012397:Rosette Formation; D013601:T-Lymphocytes
D010835:Phytohemagglutinins
Odjeljivanje limfocita iz periferne krvi ljudi na klase pomoću centrifugiranja na diskontinuiranom gradijentu.