The ribitol-phosphate-containing lipopolysaccharide from Proteus mirabilis, strain D52. Investigations on the structure of O-specific chains.
A soluble hydrophilic lipopolysaccharide, termed lipopolysaccharide II, isolated from Proteus mirabilis, strain D52 contained N-acetylglucosamine, glucose, galactose, ribitol phosphate and ethanolamine phosphate as constituents of the O-specific polysaccharide. Periodate oxidation studies were carried out on the polymer before and after dephosphorylation with hydrofluoric acid and on oligosaccharides derived from the polymer by partial acid hydrolysis. The results obtained indicate that the polysaccharide chain consists of the chemical repeating unit Gal-1,3(4)-GlcNAc-1,3-Glc-1,3-GlcNAc-, where GlcNAc stands for N-acetylglucosamine. Whereas the galactose residue is substituted at C-3 by ribitol phosphate, the glucose is substituted by ethanolamine phosphate at C-6.
D000017:ABO Blood-Group System; D000117:Acetylglucosamine / Q000032:analysis; D000942:Antigens, Bacterial; D005947:Glucose / Q000032:analysis; D008070:Lipopolysaccharides; D009844:Oligosaccharides / Q000032:analysis; D010428:Pentosephosphates / Q000032:analysis*; D011513:Proteus mirabilis / Q000032:analysis*; D013402:Sugar Alcohols / Q000032:analysis
D000017:ABO Blood-Group System; D000942:Antigens, Bacterial; D008070:Lipopolysaccharides; D009844:Oligosaccharides; D010428:Pentosephosphates; D013402:Sugar Alcohols; D005947:Glucose; D000117:Acetylglucosamine