A DNase for apurinic/apyrimidinic sites associated with exonuclease III of Hemophilus influenzae.
An endonuclease purified from Hemophilus influenzae made single strand breaks in DNA containing apurinic or apyrimidinic sites but had no detectable endonuclease activity on untreated native DNA. The new 5'-termini created at the cleavage sites were base-free deoxyribose 5-phosphate residues. The enzyme preparation also catalyzed the exonucleolytic release of 5'-mononucleotides from bihelical DNA and the hydrolysis of DNA 3'-terminal phosphomonoesters. The phosphatase-exonuclease activity was indistinguishable from that reported by Gunther and Goodgal (J. Biol. Chem. (1970) 245, 5341-5349) and resembled that of exonuclease III of Escherichia coli. The endonucleolytic and exonucleolytic activities could not be separated by electrophoresis, sedimentation, or gel filtration, and they were also affected simultaneously by mutation. The enzymatic activities appear to be functions of a single monomeric protein (M(r) = 30,000).
D001080:Apurinic Acid; D003851:Deoxyribonucleases / Q000302:isolation & purification / Q000378:metabolism*; D004720:Endonucleases / Q000302:isolation & purification / Q000378:metabolism*; D005092:Exonucleases / Q000302:isolation & purification / Q000378:metabolism*; D006193:Haemophilus influenzae / Q000201:enzymology*; D007700:Kinetics; D008970:Molecular Weight; D011119:Polynucleotides; D011685:Purine Nucleotides; D013379:Substrate Specificity
D011119:Polynucleotides; D011685:Purine Nucleotides; D001080:Apurinic Acid; D003851:Deoxyribonucleases; D004720:Endonucleases; D005092:Exonucleases