Immunity to sporozoite-induced malaria infeciton in mice. I. The effect of immunization of T and B cell-deficient mice.
The cellular basis of immunity to sporozoites was investigated by examing the effect of immunization of T and B cell-deficient C57BL/6N X BALB/c AnN F1 (BLCF1) mice compared to immunocompetent controls. Immunization of T cell-deficient (ATX-BM-ATS) BLCF1 mice with x-irradiated sporozoites did not result in the generation of protective immunity. The same immunization protocols protected all immunocompetent controls. In contrast, B cell-deficient (micron-suppressed) BLCF1 mice were protected by immunization in the majority of cases. The absence of detectable serum circumsporozoite precipitins or sporozoite neutralizing activity in the micron-suppressed mice that resisted a sporozoite challenge suggests a minor role for these humoral factors in protection. These data demonstrate a preeminent role for T cells in the induction of protective immunity in BLCF 1 mice against a P. berghei sporozoite infection.
D000818:Animals; D000831:Animals, Newborn; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology; D006224:Cricetinae; D005260:Female; D007111:Immunity, Cellular; D007114:Immunization; D007136:Immunoglobulins; D066298:In Vitro Techniques; D008212:Lymphocyte Depletion; D008288:Malaria / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008934:Mitogens / Q000494:pharmacology; D010962:Plasmodium berghei; D011247:Pregnancy; D011947:Receptors, Antigen, B-Cell / Q000032:analysis; D013154:Spleen / Q000276:immunology; D013170:Spores / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology
D007136:Immunoglobulins; D008934:Mitogens; D011947:Receptors, Antigen, B-Cell