title: Polyamine levels in Escherichia coli during nutritional shiftup and exponential growth.
abstract: At different exponential growth rates obtained either by varying the carbon source of the culture medium or limiting glucose uptake, intracellular levels of putrescine and spermidine were measured. Over a ten-fold increase in growth rate an approximately three-fold increase in putrescine level and a 3.5-fold increase in spermidine level per cell absorbance were observed. Conditions favoring an abrupt alteration in growth rate, such as occur following nutritional shiftup of Escherichia coli, resulted in a significant increase in the intracellular level of putrescine and virtually no change in the spermidine level. Because of the magnitude and the timing of the change in polyamine levels, the hypothesis that polyamines are (the components) responsible for inducing the rapid increase in the rate of RNA synthesis following nutritional shiftup is rejected.
mesh_terms: D002455:Cell Division / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D005947:Glucose / Q000378:metabolism; D007700:Kinetics; D008757:Methylglucosides / Q000494:pharmacology; D011700:Putrescine / Q000378:metabolism*; D013045:Species Specificity; D013096:Spermine / Q000378:metabolism*
chemical_list: D008757:Methylglucosides; D013096:Spermine; D005947:Glucose; D011700:Putrescine