[Transformation of mouse lymphocytes stimulated by phytohemagglutin depending on their cultivation conditions].
Conditions were elaborated to cultivate splenocytes of mice in medium-199 (Soviet production) changed a little by the addition of 200 mM of glutamine, 6 mM glucose, 60 unit/l of insulin, 5.10(-5) M 2-mercaptoethanol (final concentration), and of 5% serum of new-born calf. In such conditions of cultivation the value of the lymphocyte transformation coefficient lies within 3 and 10.
D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D010835:Phytohemagglutinins / Q000494:pharmacology*; D013154:Spleen; D013601:T-Lymphocytes / Q000187:drug effects
D003470:Culture Media; D010835:Phytohemagglutinins
Transformatsiia myshinykh limfotsitov, stumulirovannykh fitogemaggliutininom, v zavisimosti ot usloviĭ ikh kul'tivirovaniia.