Lipid synthesis in the adult dog heartworm, Dirofilaria immitis.
1. Incorporation studies with three labelled substrates--[14C]2-glycerol, [14C]1-acetate and [14C]1-oleic acid--demonstrated that adult dog heartworms can synthesize all classes of complex lipids present, including free cholesterol. 2. Diacylglycerols and phosphoglycerides were most rapidly labelled regardless of the precursor employed. 3. 14C from glycerol was found in the aqueous phase of saponified lipids, whereas that from oleic acid was in the fatty acid portion. 4. Tag from acetate was predominantly in the fatty acid portion of saponified lipids and also occurred in the unesterified fatty acids. 5. Acetate and unesterified fatty acids, as represented by oleic acid, were more readily used for lipid synthesis than was glycerol.
D000042:Absorption; D000085:Acetates / Q000378:metabolism; D000818:Animals; D050260:Carbohydrate Metabolism; D004182:Dirofilaria / Q000378:metabolism*; D004285:Dogs; D005370:Filarioidea / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism; D008055:Lipids / Q000096:biosynthesis*; D009829:Oleic Acids / Q000378:metabolism
D000085:Acetates; D008055:Lipids; D009829:Oleic Acids; D005990:Glycerol