title: Activity of murein hydrolases in synchronized cultures of Escherichia coli.
abstract: Murein hydrolase activities were analyzed in synchronized cultures of Escherichia coli B/r. Cell wall-bound murein hydrolase activities, including the penicillin-sensitive endopeptidase, increased discontinuously during the cell cycle and showed maximum activity at a cell age of 30 to 35 min (generation time, 43 min). Maximum activity was observed at the same time that the rate of cell wall synthesis reached its maximum. These oscillations depended on the termination of replication: no increase in hydrolase activity was found if deoxyribonucleic acid synthesis was inhibited at an early time in the life cycle. In contrast, the activity of another murein hydrolase that was not tightly bound to the membrane (transglycosylase) increased exponentially with time, even when deoxyribonucleic acid synthesis was inhibited.
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis; D002473:Cell Wall / Q000201:enzymology; D004269:DNA, Bacterial / Q000096:biosynthesis; D004926:Escherichia coli / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D009268:Nalidixic Acid / Q000494:pharmacology; D010447:Peptide Hydrolases / Q000378:metabolism*; D010457:Peptidoglycan
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D010457:Peptidoglycan; D009268:Nalidixic Acid; D010447:Peptide Hydrolases