Functional analysis of lymphoid cells from mouse bone marrow in vitro.
The present results show that mouse bone marrow (BM) cells responded in vitro against phytohaemagglutinin (PHA), concanavalin A (Con A) and lipopolysaccharide (LPS). In addition, BM cell suspensions contained relatively many cells with ADCC (antibody-dependent cell-mediated cytotoxicity) activity. BM cells were fractionated according to various cell surface characteristics. The results of these experiments were compared with previously published reports, showing that pre-T and pre-B cells could be separated on Ig anti-Ig columns (Ig = immunoglobulin). The combined results showed that pre-T cells may fractionate together with cells with the following characteristics: they displayed ADCC activity and had Fc receptors (FcR) and/or membrane Ig. Pre-B cells fractionated with cells with the following characteristics: they responded to LPS but had no ADCC activity. They were not adherent and/or phagocytic and were Ig-, FcR- and C3 receptor (C3R) negative.
D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity / Q000187:drug effects; D001854:Bone Marrow Cells; D002448:Cell Adhesion / Q000187:drug effects; D002458:Cell Fractionation; D002478:Cells, Cultured; D003208:Concanavalin A / Q000494:pharmacology; D005260:Female; D007501:Iron / Q000494:pharmacology; D007958:Leukocyte Count; D008070:Lipopolysaccharides / Q000494:pharmacology; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology*; D008264:Macrophages / Q000187:drug effects; D008297:Male; D051379:Mice; D008934:Mitogens; D010587:Phagocytosis / Q000187:drug effects; D010835:Phytohemagglutinins / Q000494:pharmacology; D011947:Receptors, Antigen, B-Cell; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000187:drug effects
D008070:Lipopolysaccharides; D008934:Mitogens; D010835:Phytohemagglutinins; D011947:Receptors, Antigen, B-Cell; D003208:Concanavalin A; D007501:Iron