[Splitting and rejoining simian adenovirus type 7 DNA by using Eco RI restriction endonuclease and DNA-ligase].
Optimal conditions for ligation of simian adenovirus type 7 (SA-7) DNA fragments formed under the effect of treatment of the intact molecule with Eco RI endonuclease were established. It was shown that up to 30% of the original material may be ligated and transferred into a structure with molecular weight 23 X 10(6) daltons which corresponded to the molecular weight of the intact SA-7 DNA. The data of the existence of one recognition site for Eco RI in SA-7 DNA were confirmed. The biological activity of the ligated material was demonstrated on lambda-III phage DNA.
D000256:Adenoviridae / Q000235:genetics*; D000261:Adenoviruses, Simian / Q000235:genetics*; D003090:Coliphages / Q000235:genetics; D011088:DNA Ligases; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D004279:DNA, Viral; D004926:Escherichia coli / Q000201:enzymology; D014162:Transfection
D004274:DNA, Recombinant; D004279:DNA, Viral; D004262:DNA Restriction Enzymes; D011088:DNA Ligases
Razrezanie i sshivanie DNK obez'ian'ego adenovirusa typa 7 s pomoshch'iu restriktsionnoĭ éndonukleazy Eco RI i DNK-ligazy.