Density-gradient separation of spleen-cell subpopulations from Marek's disease virus-infected chickens.
Ficoll gradient separation of spleen-cell suspensions has shown that depressed T-cell mitogen reactivity in cultures of JM-VS and MD cells results from selective depletion of mitogen-responsive T-cells.
D000818:Animals; D002469:Cell Separation / Q000379:methods; D002499:Centrifugation, Density Gradient; D002645:Chickens; D066298:In Vitro Techniques; D008213:Lymphocyte Activation; D008380:Marek Disease / Q000276:immunology*; D008934:Mitogens / Q000494:pharmacology; D012397:Rosette Formation; D013154:Spleen / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
D008934:Mitogens