Effect of proteolysis of transcriptional fidelity of reconstituted chromatin.
The effect of proteolysis on the transcriptional properties of reconstituted rat liver chromatin was studied. Within the sensitivity of currently available methods, proteolysis of chromosomal proteins by chromatin-bound protease during chromatin reconstitution has no apparent effect on: (1) number of initiation sites, (2) proportion of reiterating and unique sequences of DNA transcribed, (3) size of the RNA transcribed, and (4) transcription of DNA sequences complementary to poly(A) containing messenger RNA.
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology; D007700:Kinetics; D008099:Liver / Q000378:metabolism; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D010447:Peptide Hydrolases / Q000378:metabolism*; D051381:Rats; D014158:Transcription, Genetic
D002843:Chromatin; D012321:DNA-Directed RNA Polymerases; D010447:Peptide Hydrolases