title: Early detection of antigen and estimation of virus yield in specimens from patients with Marburg virus disease.
abstract: Autopsy specimens from patients with Marburg disease having at least 10(4.5) TCID(50) of virus per gram of tissue were found to contain sufficient fluorescent antigen-positive cells to make a specific diagnosis possible in less than 3 h. Liver, heart, spleen, and kidney tissues were found to contain significant amounts of virus. Tissue suspensions, as well as blood or serum samples, inoculated into Vero cell cultures produced virus-specific immunofluorescence within 2-5 days. At least one specimen of all virus-positive persons yielded Marburg virus-specific antigen on day 2 or 3 after inoculation. Furthermore, tissues with at least 10(5.5) TCID(50) of virus/g had Marburg antigen of sufficient titre to be used in complement fixation tests.
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis*; D002478:Cells, Cultured; D006801:Humans; D008379:Marburg Virus Disease / Q000276:immunology / Q000382:microbiology*; D029024:Marburgvirus / Q000302:isolation & purification*; D012209:Rhabdoviridae / Q000302:isolation & purification*
chemical_list: D000956:Antigens, Viral