[Evaluation of the protective action of diphosphonic compounds against a T-lymphocyte lesion by antilymphocyte serum].
Rabbit antilymphocyte serum (ALS) and complement were used in doses killing 50% of lymphocytes (staining with a 1% solution of trypan blue). In rosette-forming and blast-cell transformation reactions the counts of rosette forming and transformed cells were 60 and 24% respectively, as compared to those in controls. After adding 0.1 ml of 10 mM solution of potassium hydroxyethylene-diphosphonate belonging to a group of synthetic diphosphonates, the protective effect was observed shown by that the counts of rosette-forming and transformed lymphocytes did not differ from the control ones, wherein the reactions were produced with intact cells.
D000818:Animals; D000961:Antilymphocyte Serum / Q000494:pharmacology*; D003165:Complement System Proteins / Q000276:immunology; D004164:Diphosphonates / Q000276:immunology*; D006801:Humans; D066298:In Vitro Techniques; D008213:Lymphocyte Activation / Q000187:drug effects; D011817:Rabbits; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology
D000961:Antilymphocyte Serum; D004164:Diphosphonates; D003165:Complement System Proteins
Otsenka zashchitnogo deĭstviia difosfonovykh soedineniĭ ot povrezhdeniia T-limfotsitov antilimfotsitarnoĭ syvorotki.