Production of specific antibodies to contractile proteins, and their use in immunofluorescence microscopy. II. Species-specific and species-non-specific antibodies to smooth and striated chicken muscle actin.
The injection of rabbits with insoluble or soluble G-actin from chicken smooth or striated muscle will produce antibodies that are equally reactive, and species and tissue non-specific in immunoprecipitation, immunofluorescence and actin-activated Mg2+-ATPase inhibition tests. These antibodies have been used for the identification of actin-containing fibrils in a variety of tissues. When G-actins from chicken smooth or striated muscle are immobilized by chemical linkage to Affi-Gel 702 microbeads, their immunogenicity is increased, but the antibodies obtained against them are species-specific and will only react with actin and actin-containing structures from chicken and are therefore limited in use. It is concluded from this work that insoluble G-actin is the preferable immunogen to obtain precipitating antibodies for wide use.
D000199:Actins / Q000032:analysis* / Q000276:immunology; D000818:Animals; D000906:Antibodies / Q000302:isolation & purification; D000917:Antibody Formation; D000918:Antibody Specificity; D002645:Chickens; D005455:Fluorescent Antibody Technique; D009130:Muscle, Smooth / Q000032:analysis* / Q000276:immunology; D009132:Muscles / Q000032:analysis* / Q000276:immunology; D009218:Myosins / Q000032:analysis; D009928:Organ Specificity; D011817:Rabbits; D013045:Species Specificity
D000199:Actins; D000906:Antibodies; D009218:Myosins