A streamlined technique for on-grid electron immunocytochemistry.
The unlabelled antibody enzyme technique for on-grid immunostaining demands exacting conditions of cleanliness. These can be met with minimal trouble by two technical modifications. Large supplies of all the solutions are millipore-filtered in advance and are then stored frozen in sealed plastic bags and allowed to thaw when required. A disposable, contamination-free surface with depressions to hold drops of staining solution is prepared from a strip of parafilm.
D007124:Immunoenzyme Techniques / Q000295:instrumentation*; D008854:Microscopy, Electron