title: "Active" one-carbon generation in Saccharomyces cerevisiae.
abstract: A new mutation introducing a one-carbon requirement (e.g., formate) for the glycine-supplemented growth of a serine-glycine auxotroph (ser1) was correlated with a lack of glycine decarboxylase activity. The presence of oxalate decarboxylase activity or glyoxylate decarboxylase activity did not overcome the one-carbon requirement. Another mutation characterized by the absence of oxalate decarboxylase activity did not introduce a one-carbon requirement. The presence and physiological significance of glycine decarboxylase activity in Saccharomyces are thus inferred.
mesh_terms: D000085:Acetates / Q000378:metabolism; D000445:Aldehyde Oxidoreductases / Q000378:metabolism; D002262:Carboxy-Lyases / Q000378:metabolism; D005561:Formates / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D005998:Glycine / Q000378:metabolism; D006038:Glyoxylates / Q000378:metabolism; D008928:Mitochondria / Q000201:enzymology; D009154:Mutation; D010070:Oxalates / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000378:metabolism*; D012694:Serine / Q000378:metabolism
chemical_list: D000085:Acetates; D005561:Formates; D006038:Glyoxylates; D010070:Oxalates; D012694:Serine; D000445:Aldehyde Oxidoreductases; D002262:Carboxy-Lyases; D005947:Glucose; D005998:Glycine