[Detection of the localization of C1. botulinum antigens in regional lymph node cells by the immunofluorescence technic].
As a result of the study of lacalization of the C1. botulinum toxoids of the A, B, and E types in cells of the regional lymph nodes of rabbits by the indirect Coons' method and by the smear-print method it was revealed that different types of lymphoid tissue cells took part in the ingestion and digestion of these antigens; the antigens of the A and B types were at first revealed in the cytoplasm of pseudoeosinophils, and then in the macrophages. The antigen of the E type was revealed in the course of the whole experiment in the macro phages of the regional lymph node, although the number of pseudoeosinophils also increased. Apparently there was a different activity of pseudoeosinophils against the C1. botulinum toxoids, the types A, B and E.
D000818:Animals; D001906:Botulism / Q000276:immunology / Q000517:prevention & control*; D005455:Fluorescent Antibody Technique; D008198:Lymph Nodes / Q000276:immunology*; D008264:Macrophages / Q000276:immunology; D011817:Rabbits; D013045:Species Specificity; D014121:Toxoids; D014611:Vaccination
D014121:Toxoids
Vyiavlenie lokalizatsii antigenov C1. botulinum v kletkakh regionarnogo limfaticheskogo uzla metodom immunofluorestsentsii