[On the role of N7 atoms of guanosine in tRNA-Phe (E. coli) in interaction with ribosomes].
The influence of alkylation of phe-tRNAPhe (E. coli) with 2',3'-O-[4-(N-2-chloroethyl-N-methylamino)-benzylidene]-uridine - 5' - methylphosphate on its ability to participate in non-enzymatic complex formation with ribosomes and poly-U was investigated. Phe-tRNAsPhe, containing alkylated guanosines at different positions, including anticodone, are active in binding with ribosomes. It is concluded, that N7 nitrogens of guanosine of the tRHAPhe are not elements, significant for the interaction with ribosomes.
D000478:Alkylation; D000926:Anticodon / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D006151:Guanosine / Q000378:metabolism*; D009588:Nitrogen Mustard Compounds; D010649:Phenylalanine; D011072:Poly U / Q000378:metabolism; D012329:RNA, Bacterial / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*; D013329:Structure-Activity Relationship; D014500:Uracil Nucleotides
D000926:Anticodon; D009588:Nitrogen Mustard Compounds; D012329:RNA, Bacterial; D014500:Uracil Nucleotides; D006151:Guanosine; D011072:Poly U; D010649:Phenylalanine; D012343:RNA, Transfer
O roli N-7 atomov guanozina tRNKphe (E. coli) v ee vzaimodeĭstvii s ribosomami.