title: Effects of in vivo priming on in vitro induction of cytotoxicity. I. Non-specific augmentation by in vivo presensitization with allogeneic or xenogeneic cells.
abstract: In vivo presensitization of donor mice of responding cells with third party cellular antigens augmented in vitro generation of cytotoxic T lymphocytes in allogeneic and xenogeneic combinations. In vitro induction of detectable cytotoxicity in presensitized responding cells required the incubation period needed for in vitro primary response. However, such cytotoxic T lymphocytes were generated after in vitro stimulation with monolayers of methylcholanthrene-induced tumor cells, UV-irradiated or heated spleen cells which had proved to be effective in secondary but not in primary response. Presensitized responding cells exposed to 600R-irradiation did not augment in vitro induction of cytotoxicity in normal responding cells. The augmenting effect of presensitized responding cells may be attributable to radiosensitive T cells which are in a transitional state in differentiation from typical unprimed cells to typical primed cells.
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000276:immunology*; D046508:Culture Techniques; D003602:Cytotoxicity, Immunologic; D005260:Female; D006358:Hot Temperature; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009368:Neoplasm Transplantation; D051381:Rats; D012513:Sarcoma, Experimental / Q000276:immunology*; D013154:Spleen; D013601:T-Lymphocytes / Q000276:immunology* / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D000951:Antigens, Neoplasm