Norvaline accumulation by regulatory mutants of Serratia marcescens.
A gene coding for desensitized L-threonine dehydratase was transduced with phage PS20 into a leucine accumulator of Serratia marcescens Sr41. The transductant converted L-threonine to alpha-ketobutyrate, a precursor of both norvaline and isoleucine. An isoleucine-valine auxotroph of the transductant accumulated large amount of norvaline from L-threonine as well as from D-threonine.
D002474:Cell-Free System; D003470:Culture Media; D005246:Feedback; D007532:Isoleucine / Q000096:biosynthesis; D007930:Leucine / Q000096:biosynthesis; D009154:Mutation; D012706:Serratia marcescens / Q000201:enzymology / Q000378:metabolism*; D013913:Threonine Dehydratase / Q000378:metabolism; D014161:Transduction, Genetic; D014633:Valine / Q000031:analogs & derivatives* / Q000096:biosynthesis
D003470:Culture Media; D007532:Isoleucine; D013913:Threonine Dehydratase; D007930:Leucine; D014633:Valine