Hairy cell leukemia: effect of 'hairy' cells on normal granulopoiesis in vitro.
The effect of 'hairy cells' (HC) on proliferation of normal marrow granulocytic progenitor cells (CFU-C) was studied in vitro, using cells obtained from the blood, spleen, and bone marrow from eight patients with hairy cell leukemia (HCL). Hairy cells of hairy-cell-conditioned media (HCCM) did not stimulate proliferation of normal CFU-C. Furthermore, HC and HCCM did not inhibit the growth of CFU-C induced by normal colony stimulating factor (CSF) or by feeder layers of normal blood leukocytes. These results confirm previous reports showing the inability of HC to produce CSF and suggest that inhibition of CFU-C proliferation, as measured by this technique, is not an important mechanism in the neutropenia observed in HCL.
D000368:Aged; D001772:Blood Cell Count; D001854:Bone Marrow Cells; D002455:Cell Division; D003114:Colony-Forming Units Assay; D003115:Colony-Stimulating Factors / Q000494:pharmacology; D003470:Culture Media; D005260:Female; D006098:Granulocytes / Q000502:physiology*; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D006801:Humans; D066298:In Vitro Techniques; D007943:Leukemia, Hairy Cell / Q000097:blood*; D008297:Male; D008875:Middle Aged; D009503:Neutropenia / Q000209:etiology
D003115:Colony-Stimulating Factors; D003470:Culture Media