Re-examination of F plasmid replication in a dnaC mutant of Escherichia coli.
The replication of an F' plasmid in a dnaC mutant, thermolabile for initiation of chromosomal replication, has been re-examined using a novel DNA-DNA annealing assay. Plasmid replication ceases rapidly at non-permissive conditions, consistent with a direct role for the dnaC product in the replication of F.
D003227:Conjugation, Genetic; D004261:DNA Replication; D004926:Escherichia coli / Q000378:metabolism*; D005111:Extrachromosomal Inheritance; D005144:F Factor; D005838:Genotype; D009154:Mutation; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D013045:Species Specificity; D013104:Spheroplasts / Q000378:metabolism