Blocking of primary in vitro antibody responses to thymus-independent and thymus-dependent antigens with antiserum specific for IgM or IgD.
The effect of anti-mu and anti-delta on the primary in vitro IgM response of murine splenocytes to thymus-dependent (trinitrophenylated erythrocytes) and thymus-independent (trinitrophenylated brucella) forms of trinitrophenyl was studied. The results indicate that either anti-mu or anti-delta can block the response of adult splenocytes to the thymus-dependent antigen. The thymus-dependent responses of neonatal splenocytes that bear a low concentration of IgD were also abrogated by treatment with anti-delta. In contrast, anti-mu, but not anti-delta, blocked the response of adult splenocytes to the thymus-independent antigen used. These results indicate that both IgM and IgD are receptors required for triggering cells by a thymus-dependent antigen but that only IgM receptors are required for triggering by the thymus-independent antigen used.
D000818:Animals; D000831:Animals, Newborn / Q000276:immunology; D000888:Antibodies, Anti-Idiotypic; D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology*; D007072:Immunoglobulin D; D007075:Immunoglobulin M; D007152:Immunologic Capping; D066298:In Vitro Techniques; D051379:Mice; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*; D014301:Trinitrobenzenes / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D007072:Immunoglobulin D; D007075:Immunoglobulin M; D014301:Trinitrobenzenes