[Synthesis and properties of carrier-fixed enzymes. VII. Linking of glucoamylase to dialdehyde cellulose, carboxymethylcellulose hydrazide and carboxymethylcellulose azide].
Glucoamylase (alpha-1,4-glucan glucohydrolase, EC 3.2.1.3) has been covalently linked to dialdehyde cellulose resulting in an immobilized enzyme containing 0.98% protein and an activity of 4.5 mg of the native enzyme per g of matrix, i.e. 46% relative activity. The complex lost its activity in continuous and batch hydrolysis of starch at 55 degrees C down to a limit of 18% of its original value. In contrast, the activity of the complex did not change when working at a temperature of 25 degrees C. Glucoamylase-carboxymethylcellulose complexes synthesized via carboxymethylcellulose hydrazide and azide, in contrast to MAEDA und SUZUKI [1], showed only an activity of 1 mg of the native enzyme per g of matrix. We did not succeed in coupling periodate-oxidized glucoamylase to carboxymethylcellulose hydrazide because the enzyme used lost nearly all of its activity already during periodate oxidation.
D001386:Azides; D002266:Carboxymethylcellulose Sodium; D002482:Cellulose; D004800:Enzymes, Immobilized; D005087:Glucan 1,4-alpha-Glucosidase / Q000138:chemical synthesis*; D005959:Glucosidases / Q000138:chemical synthesis*; D006868:Hydrolysis; D008747:Methylcellulose / Q000031:analogs & derivatives; D010084:Oxidation-Reduction
D001386:Azides; D004800:Enzymes, Immobilized; D002482:Cellulose; D008747:Methylcellulose; D005959:Glucosidases; D005087:Glucan 1,4-alpha-Glucosidase; D002266:Carboxymethylcellulose Sodium
Synthese und Eigenschaften trägerfixierten Enzyme. VII. Fixierung von Glukoamylase an Dialdehydzellulose. Karboxymethylzellulosehydrazid und Karboxymethylzelluloseazid.