Mitogenic stimulation of human lymphocyte subpopulations by Lathyrus odoratus lectin.
Investigation was made of the mitogenic activation by Lathyrus odoratus (LATH) lectin and phytohaemagglutinin (PHA) of human T-enriched and non-T lympohcytes isolated by nylon wool filtration and sheep erythrocyte rosette depletion, respectively. LATH stimulated only weakly the T-enriched and non-T fractions as compared to PHA which strongly stimulated the T-enriched population and weakly stimulated the non-T cells. Removal of phagocytic cells from the unfractionated lymphocytes caused only a slight reduction in the response to LATH. The responses of mixtures of T-enriched and non-T lymphocytes were similar to that of unfractionated lymphocytes. The LATH lectin seems, therefore, to be neither a pure T nor a pure non-T cell mitogen, since both populations must be present for optimal response. When alpha-methyl-D-mannoside was added to unfractionated lymphocytes together with LATH, there was a strong inhibition of thymidine incorporation. D-glucose and N-acetyl-D-glucosamine diminished the stimulation effect to a lesser degree.
D002478:Cells, Cultured; D007887:Fabaceae; D006801:Humans; D037102:Lectins / Q000494:pharmacology*; D008213:Lymphocyte Activation; D008212:Lymphocyte Depletion; D008214:Lymphocytes / Q000187:drug effects*; D008934:Mitogens; D009005:Monosaccharides / Q000494:pharmacology; D010835:Phytohemagglutinins / Q000494:pharmacology; D037121:Plant Lectins; D010946:Plants, Medicinal; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology
D037102:Lectins; D008934:Mitogens; D009005:Monosaccharides; D010835:Phytohemagglutinins; D037121:Plant Lectins