Alterations in granulation tissue growth induced in vivo by lymphocytes from adjuvant-diseased rats.
Lymph node cells from Lewis and Wistar rats, treated 9 or 11 days previously with Freund's complete (FCA) or incomplete adjuvant (FIA), were transferred into polyether sponges implanted subcutaneously into syngeneic, recipient rats. FCA-treated lymphocytes enhanced or reduced granuloma formation (measured after 8 days), when compared with FIA-treated controls, depending on the strain of mycobacterium present in the FCA. The stimulatory effects of lymphocytes from FCA-treated, Lewis rats were abolished by pre-incubation with mitomycin C (25 microgram/ml). Whole serum and isolated serum immunoglobulin from adjuvant-diseased rats had no effect on the sponge granulomas. These data confirm that cell-mediated immunity is involved in the articular granuloma formation of adjuvant arthritis.
D000818:Animals; D001168:Arthritis / Q000150:complications*; D001169:Arthritis, Experimental / Q000150:complications*; D005620:Freund's Adjuvant; D006099:Granuloma / Q000209:etiology*; D007116:Immunization, Passive; D007136:Immunoglobulins; D008198:Lymph Nodes / Q000276:immunology; D008214:Lymphocytes; D008297:Male; D009161:Mycobacterium / Q000276:immunology; D051381:Rats; D011917:Rats, Inbred Lew
D007136:Immunoglobulins; D005620:Freund's Adjuvant