Measurement of anti-double-stranded DNA antibodies in major immunoglobulin classes.
A solid-phase radioimmunoassay for quantitating anti-double-stranded deoxyribonucleic acid antibodies (anti-dsDNA) in IgG, IgM and IgA classes has been devised. A distinct feature of the method is an application of polystyrene tubes coated with poly-L-lysine, through which dsDNA could be bound firmly to a solid phase. Studies on patients' sea as well as normal sera revealed that anti-dsDNA was not qualitatively but quantitatively characteristic of systemic lupus erythematosus (SLE) and that IgG anti-dsDNA levels correlated well with the disease activity.
D000906:Antibodies / Q000032:analysis* / Q000378:metabolism; D000888:Antibodies, Anti-Idiotypic / Q000378:metabolism; D004247:DNA / Q000276:immunology*; D004355:Drug Stability; D006801:Humans; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007700:Kinetics; D008180:Lupus Erythematosus, Systemic / Q000097:blood; D008851:Micropore Filters; D011137:Polystyrenes; D011863:Radioimmunoassay / Q000379:methods*; D012217:Rheumatoid Factor / Q000494:pharmacology
D000906:Antibodies; D000888:Antibodies, Anti-Idiotypic; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011137:Polystyrenes; D004247:DNA; D012217:Rheumatoid Factor