The distribution of adenosine deaminase among lymphocyte populations in the rat.
Adenosine deaminase (ADA) activity was determined in young rat lymphocyte populations. The ADA-specific activity (per 10(8) cells and per milligram protein) was 3- to 10-fold higher in thymocytes than in lymphocytes from thoracic duct, lymph node, spleen, and bone marrow. The high ADA activity in thymocytes appeared to be preferentially associated with cortical thymocytes. Enrichment or depletion of cortical thymocytes by density gradient centrifugation, cortisone treatment, or  selective lysis with anti-Thy-1 plus complement resulted in parallel increases or decreases in ADA levles. These results also suggested that medullary thymocytes have ADA levels similar to those of peripheral lymphocytes. "Immature" cortical thymocytes and thymocyte progenitors appeared to have low ADA activity; low enzyme levels were found in fetal thymus at 16 days of embryonic life, in the early phases of thymus regeneration, and in a "null" cell population isolated from bone marrow. This study demonstrates that ADA activity varies markedly during T lymphocyte differentiation and suggests that fundamental differences in nucleotide metabolism may exist in T cells at different stages of development.
D000243:Adenosine Deaminase / Q000378:metabolism*; D000305:Adrenal Cortex Hormones / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn; D001853:Bone Marrow / Q000201:enzymology; D003165:Complement System Proteins; D003602:Cytotoxicity, Immunologic; D005260:Female; D005333:Fetus / Q000201:enzymology; D008214:Lymphocytes / Q000201:enzymology*; D008297:Male; D009700:Nucleoside Deaminases / Q000378:metabolism*; D011247:Pregnancy; D051381:Rats; D011917:Rats, Inbred Lew; D013601:T-Lymphocytes / Q000201:enzymology; D013897:Thoracic Duct / Q000201:enzymology; D013950:Thymus Gland / Q000201:enzymology
D000305:Adrenal Cortex Hormones; D003165:Complement System Proteins; D009700:Nucleoside Deaminases; D000243:Adenosine Deaminase