Pronase pretreatment of tissue sections enhances sensitivity of the unlabelled antibody-enzyme (PAP) technique.
Treatment of dewaxed sections of formalin-fixed paraffin embedded material with 0.1% pronase for 15 min at 37 degrees C prior to the performance of the unlabelled antibody-enzyme (PAP) procedure greatly enhanced its sensitivity. This was shown by detection of immunoglobulin within plasma cells in chronic gastritis specimens.
D000818:Animals; D005557:Formaldehyde / Q000378:metabolism; D005753:Gastric Mucosa / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D011402:Pronase / Q000378:metabolism*; D011817:Rabbits
D005557:Formaldehyde; D011402:Pronase