title: Progesterone production by cultured preantral rat granulosa cells? Stimulation by androgens.
abstract: Progesterone (P) production by isolated rat granulosa cells from preantral follicles was enhanced by addition of androgens to the tissue culture medium. Testosterone (T) at 10(-7), 10(-6), and 10(-4)M as well as 10(-6)M dihydrotestosterone (DHT) increased P production 400 to 700% over paired control cultures. Human chorionic gonadotropin (100 mIU/ml) and 17beta-estradiol (7.8 X 10(-10M) had no effect on P production. P was identified by both a specific radioimmunoassay and sephadex LH-20 column chromatography. The stimulatory influence of T and DHT on these preantral follicular cells is consistent with a direct role for androgens in granulosa cell differentiation.
mesh_terms: D000728:Androgens / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D013196:Dihydrotestosterone / Q000494:pharmacology; D004958:Estradiol / Q000096:biosynthesis* / Q000494:pharmacology; D005260:Female; D006107:Granulosa Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008858:Microscopy, Phase-Contrast; D006080:Ovarian Follicle / Q000378:metabolism*; D011374:Progesterone / Q000096:biosynthesis*; D051381:Rats; D013739:Testosterone / Q000494:pharmacology
chemical_list: D000728:Androgens; D006063:Chorionic Gonadotropin; D013196:Dihydrotestosterone; D013739:Testosterone; D011374:Progesterone; D004958:Estradiol