Rapid screening method for enterotoxigenic Escherichia coli.
A rapid screening method for detection of Escherichia coli producing heat-labile enterotoxin is described. Single colonies are transferred directly from primary culture plates into 96-well microculture plates containing 0.3 ml of brain heart infusion broth in each well. After 24 h at 37 degrees C, each brain heart infusion broth culture is assayed by the miniculture method in the corresponding well of a microculture plate in which Y-1 mouse adrenal tumor cells have been grown. All enterotoxigenic isolates detected by this method were confirmed in the assay but with culture supernatants.
D000310:Adrenal Gland Neoplasms; D000328:Adult; D000818:Animals; D001431:Bacteriological Techniques; D002460:Cell Line; D002648:Child; D003470:Culture Media; D004768:Enterotoxins / Q000096:biosynthesis*; D004926:Escherichia coli / Q000378:metabolism*; D005243:Feces / Q000382:microbiology*; D006801:Humans; D051379:Mice; D013696:Temperature
D003470:Culture Media; D004768:Enterotoxins