Specialized transducing phage for the initiation factor 3 gene in Escherichia coli.
A previously isolated thermosensitive mutant [Springer, M., Graffe, M. & Grunberg-Manago, M. (6977) Mol. Gen. Genet. 151, 17-26] exhibits two defects in vitro, one in the initiation factor IF3 and the other in the L-phenylalanine: tRNA-Phe ligase (EC 6.1.1.20). Specialized lambda transducing phages that transduced this mutant to thermoresistance were selected. In vitro studies showed that the transductants had a normal IF3 activity. One of these transducing phages was shown to code for a protein synthesized under the control of Escherichia coli promoters, which has the same molecular weight as IF3. This protein crossreacts specifically with IF3 antisera and comigrates with pure IF3 in a two-dimensional gel system.
D001426:Bacterial Proteins / Q000096:biosynthesis; D003090:Coliphages / Q000235:genetics*; D004274:DNA, Recombinant / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D005838:Genotype; D007700:Kinetics; D008242:Lysogeny; D008970:Molecular Weight; D010448:Peptide Initiation Factors; D014176:Protein Biosynthesis; D014161:Transduction, Genetic; D014764:Viral Proteins / Q000096:biosynthesis
D001426:Bacterial Proteins; D004274:DNA, Recombinant; D010448:Peptide Initiation Factors; D014764:Viral Proteins