Direct cytotoxicity against chicken erythrocytes in mice. II. Nature of immunoglobulin on cytotoxic T lymphocytes.
Direct cytotoxicity raised at an early interval after immunization with chicken erythrocytes (CRBC) has been ascribed to O-positive, immunoglobulin (Ig)-bearing lymphocytes. Cytotoxicity of such effector cells was not affected by trypsinization. Cytotoxity was reduced by the treatment of effector cells with anti-IgG serum and complement or by the addition of anti-IgG serum to culture medium for cytotoxicity test. When the effector cells were treated with trypsin before cytotoxicity test, such procedures with anti-IgG serum did not affect their cytotoxicity. Ig molecules on cytotoxic T lympohcytes may not be an essential element for antigen recognition. This explanation may be supported by the fact that cytotoxicity against CRBC could be raised in 600 rad irradiation, thymus-cell transferred mice in the absence of antibody production.
D000818:Animals; D000917:Antibody Formation; D000961:Antilymphocyte Serum; D002645:Chickens / Q000276:immunology; D003165:Complement System Proteins; D003602:Cytotoxicity, Immunologic; D004912:Erythrocytes / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology; D007136:Immunoglobulins / Q000032:analysis*; D008297:Male; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology*; D014357:Trypsin
D000961:Antilymphocyte Serum; D007074:Immunoglobulin G; D007136:Immunoglobulins; D003165:Complement System Proteins; D014357:Trypsin