Light emission techniques for the microestimation of femtogram levels of peroxidase. Application to peroxidase (and other enzymes)-coupled antibody-cell antigen interactions.
D000818:Animals; D000937:Antigen-Antibody Reactions; D004305:Dose-Response Relationship, Drug; D049454:Firefly Luciferin / Q000378:metabolism; D005949:Glucose Oxidase / Q000276:immunology; D006735:Horseradish Peroxidase / Q000032:analysis*; D006861:Hydrogen Peroxide / Q000378:metabolism; D007093:Imidazoles / Q000494:pharmacology; D007124:Immunoenzyme Techniques; D008027:Light; D008156:Luciferases / Q000276:immunology; D008165:Luminol / Q000378:metabolism; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008832:Microchemistry; D010544:Peroxidases / Q000032:analysis*; D010777:Photochemistry
D007093:Imidazoles; D008165:Luminol; D049454:Firefly Luciferin; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D006735:Horseradish Peroxidase; D010544:Peroxidases; D008156:Luciferases