Proteinase-sensitive release of enzymes from pancreatic microsomal fraction.
Suspensions of rat pancreatic microsomal fraction release alpha-amylase and ribonuclease on incubation at 37 degrees C, but not at 2 degrees C. The release is abolished by proteolytic enzymes. Ribonuclease associated with the microsomal fraction is protected from subtilisin BPN' attack, but is sensitive after release.
D000818:Animals; D066298:In Vitro Techniques; D008297:Male; D008861:Microsomes / Q000187:drug effects / Q000201:enzymology*; D010179:Pancreas / Q000201:enzymology*; D010447:Peptide Hydrolases / Q000494:pharmacology*; D051381:Rats; D012260:Ribonucleases / Q000378:metabolism; D013381:Subtilisins / Q000494:pharmacology; D000516:alpha-Amylases / Q000378:metabolism
D012260:Ribonucleases; D000516:alpha-Amylases; D010447:Peptide Hydrolases; D013381:Subtilisins