title: A hydrostatic device for tissue dehydration.
abstract: A simple device for mixing water and organic solvents can be easily constructed to dehydrate tissue for electron microscopy. The device utilizes hydrostatic leveling between a reservoir and a mixing chamber to produce a continuously increasing concentration of dehydrating solvent. The rate of dehydration may be regulated by geometry and/or outflow rate. A prototype model is described which compactly incorporates the solvent reservoirs and a tissue tray that can accommodate ten tissue specimens in stainless steel mesh baskets. The device can be fabricated cheaply and replaces manual dehydration.
mesh_terms: D003890:Desiccation / Q000295:instrumentation*; D006652:Histological Techniques; D006874:Hydrostatic Pressure; D008722:Methods; D008854:Microscopy, Electron; D012997:Solvents
chemical_list: D012997:Solvents