Guinea-pig lymphotoxin resistant L-cell sublines.
Guinea-pig lymphotoxin (GLT)-resistant L-cell sublines were isolated, and the altered properties were characterized. Two of them (No. 1 and No. 2 cells) showed the lowest capacity to absorb GLT and substantially reduced ability to bind labelled Ricinus communis agglutinin (RcA). Both the cell-surface proteins and glycoproteins of No. 2 cells were found to be quite different from those of parental L cells. The glycoprotein of molecular weight 140,000, which specifically bound to RcA-Sepharose, was missing in the No. 2 cells extract. These results indicate that the glycoprotein of molecular weight 140,000 is the most probable candidate for the receptor molecule for GLT, and that No. 2 cells and probably No. 1 cells are GLT receptor-deficient cells.
D000818:Animals; D001665:Binding Sites; D002470:Cell Survival / Q000187:drug effects; D004351:Drug Resistance; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000032:analysis; D006168:Guinea Pigs / Q000276:immunology; D007739:L Cells / Q000187:drug effects* / Q000378:metabolism; D037102:Lectins / Q000378:metabolism; D008233:Lymphotoxin-alpha / Q000494:pharmacology*; D008565:Membrane Proteins / Q000032:analysis; D009844:Oligosaccharides / Q000032:analysis; D011955:Receptors, Drug
D006023:Glycoproteins; D037102:Lectins; D008233:Lymphotoxin-alpha; D008565:Membrane Proteins; D009844:Oligosaccharides; D011955:Receptors, Drug