Detection of thymic surface antigens and radioactive labeling of mouse lymphoid cells.
A method for the permanent and simultaneous detection of tissue-specific surface antigens and internal radioactive labeling of mouse lymphoid cells is described. Target cells were first reacted with a mouse-derived "antithymocyte serum", incubated with peroxidase-conjugated rabbit serum against mouse immunoglobulins and placed in a substrate solution that leads to staining of the antigen-positive cells. Radioactive labeling was demonstrated by autoradiography performed after the antigen assay. More than 90% of antigen-positive thymocytes could be specifically stained in the assay without staining of similarly treated antigen-negative cells. Autoradiographic grains could be detected over both antigen-positive and antigen-negative cells.
D000818:Animals; D000941:Antigens / Q000032:analysis*; D001853:Bone Marrow / Q000276:immunology; D006651:Histocytochemistry; D007124:Immunoenzyme Techniques / Q000379:methods; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D009928:Organ Specificity; D013154:Spleen / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology*
D000941:Antigens