Serological characterization of rat leukemia lines, DBLA-1, -6, and -9.
Three lines of rat leukemia, DBLA-1, -6, and -9, were studied serologically by complement-dependent cytotoxicity test. DBLA-1, -6, and -9 were killed by anti-lymphocyte sera, anti-Thy-1.1 sera, and rabbit anti-rat brain sera absorbed with AKR/J brain. However, anti-T and anti-B lymphocyte sera had no cytotoxic effect on DBLA-1, -6, and -9. Furthermore, DBLA-6 and -9 did not absorb the cytotoxic activity of anti-T serum on thymocytes, while DBLA-1 slightly absorbed the cytotoxic activity. These results indicate that DBLA-1, -6, and -9 are of lymphoid origin and possess rat Thy-1 antigens, but lack mature T-lymphocyte antigens. On the other hand, peroxidase-positive myelogenous leukemia L1005 failed to react with any of the antisera used.
D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis; D000961:Antilymphocyte Serum / Q000276:immunology; D001921:Brain / Q000276:immunology; D002460:Cell Line; D003601:Cytotoxicity Tests, Immunologic; D005260:Female; D007942:Leukemia, Experimental / Q000276:immunology*; D051381:Rats; D013601:T-Lymphocytes / Q000276:immunology
D000951:Antigens, Neoplasm; D000961:Antilymphocyte Serum