Isolation and characterization of a rabbit T lymphocyte-specific antigen.
Rabbit T lymphocytes may be differentiated from B lymphocytes by the presence of a T lymphocyte-specific surface antigen. This unique antigen has been extracted from the plasma membrane of rabbit thymocytes by 3 M KCl. The presence of the antigen in the membrane extract was demonstrated by inhibition of cytotoxicity with goat anti-rabbit T cell serum (ATS). The crude membrane extract was fractionated by gel electrophoresis and the fractions containing the T cell antigen identified by inhibition of cytotoxic ATS and by passive hemagglutination. The purified T cell antigen was found to have a m.w. of approximately 12,000 and contained approximately 2.5% carbohydrate. Evidence was also obtained to suggest that the rabbit T cell antigen exists in multiple forms, each having the same m.w. but exhibiting different electrophoretic characteristics.
D000818:Animals; D000941:Antigens / Q000302:isolation & purification*; D002241:Carbohydrates; D002457:Cell Extracts / Q000494:pharmacology; D002462:Cell Membrane / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D004591:Electrophoresis, Polyacrylamide Gel; D006386:Hemagglutination Tests; D007525:Isoelectric Focusing; D008970:Molecular Weight; D011817:Rabbits; D013601:T-Lymphocytes / Q000276:immunology*
D000941:Antigens; D002241:Carbohydrates; D002457:Cell Extracts