Isolated retinas synthesize visual pigments from tetinol congeners delivered by liposomes.
Isolated vertebrate retinas bathed in circulating Ringer solution cannot regenerate all of their bleached visual pigments. When dioleoyl-lecithin vesicles containing certain retinol congeners are added to the Ringer solution, such retinas begin to regenerate pigment immediately. The visual pigment of a bleached perfused retina can now be restored fully, making the isolated retina an independent unit for study. Loposomes can protect oxygen-sensitive, lipid-soluble substances and deliver them to living cells.
D000818:Animals; D001001:Anura; D066298:In Vitro Techniques; D008081:Liposomes; D010477:Perfusion; D010713:Phosphatidylcholines; D011894:Rana pipiens; D012160:Retina / Q000378:metabolism*; D012168:Retinal Pigments / Q000096:biosynthesis*; D012172:Retinaldehyde / Q000008:administration & dosage / Q000378:metabolism; D012243:Rhodopsin / Q000096:biosynthesis*; D014801:Vitamin A / Q000008:administration & dosage / Q000378:metabolism*
D008081:Liposomes; D010713:Phosphatidylcholines; D012168:Retinal Pigments; D014801:Vitamin A; D012243:Rhodopsin; D012172:Retinaldehyde