title: Constant post-irradiation repopulation rates and linear relationship between cellular blood response and number of transplanted bone marrow cells in inbred mice.
abstract: Graded doses of syngeneic bone marrow cells were transplanted into lethally irradiated mice. Repopulation curves of peripheral blood granulocytes and platelets were apparently exponential and parallel after doses larger than 5 X 10(5) cells. The blood platelet Td was reduced from 111 h to 53-57 h, and granulocyte Td from 57 to 40 h in transplanted groups. The mean blood cell counts were reproducible enough to be used as a biological assay of the amount of bone marrow cells transplanted. Linear relationship between increment of blood cells up to day 16 and number of bone marrow cells transplanted on day 1 was demonstrated (1,200 granulocytes and 14,300 platelets/mul blood per 10(5) bone marrow cells). The linearity suggested a mean Td less than 22.5 h of proliferating bone marrow cells, and allowed a rough estimation of mouse bone marrow stem cell radiosensitivity (Do 76 rad).
mesh_terms: D000818:Animals; D001772:Blood Cell Count; D001792:Blood Platelets / Q000502:physiology / Q000528:radiation effects; D001853:Bone Marrow / Q000528:radiation effects; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D006098:Granulocytes / Q000502:physiology / Q000528:radiation effects; D006410:Hematopoiesis / Q000528:radiation effects*; D008214:Lymphocytes / Q000502:physiology / Q000528:radiation effects; D051379:Mice; D008809:Mice, Inbred C3H; D008811:Mice, Inbred DBA; D011833:Radiation Injuries, Experimental / Q000503:physiopathology*; D012038:Regeneration; D014185:Transplantation, Isogeneic