In vitro antibody response to influenza virus. I. T cell dependence of secondary response to hemagglutinin.
A good secondary IgG response to the hemagglutinin (HA) of influenza virus has been obtained in vitro in Marbrook-type cultures of influenza-primed mouse spleen cell suspensions stimulated with inactivated influenza virus. Anti-HA antibody was quantitated by a solid phase radioimmunoassay (RIA) by using purified HA as substrate. The T dependence of this secondary response was shown by depletion of T cells and reconstitution with a source of primed or unprimed T cells. The help given by T cells primed to the homologous virus was many times greater than that given by unprimed T cells, although the latter was significant. The system described will allow investigation of the specificity requirements of helper T cells engaged in the anti-HA response.
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D000918:Antibody Specificity; D001666:Binding Sites, Antibody; D002478:Cells, Cultured; D005260:Female; D006389:Hemagglutinins, Viral / Q000276:immunology*; D007156:Immunologic Memory; D009980:Influenza A virus / Q000276:immunology*; D007700:Kinetics; D008297:Male; D051379:Mice; D011863:Radioimmunoassay; D013601:T-Lymphocytes / Q000276:immunology*
D000914:Antibodies, Viral; D006389:Hemagglutinins, Viral