[Biosynthesis of pyridoxal-5'-phosphate in lymphocytes stimulated by alloantigens in vitro].
Dynamics of alterations in pyridoxal kinase activity (key enzyme in biosynthesis of pyridoxal-5'-phosphate) was studied in lymphocytes of mice BALB/c, stimulated by irradiated cells from spleen of mice C3H in vitro. The cytolytic activity of lymphocytes was estimated by amount of Na251CrO4 realised from lysed cell-targets into medium. Maintenance of animals on a diet without pyridoxine within 1-3 weeks did not affect on the capacity of lymphocytes to proliferative in vitro and on their cytolytic activity. At the same time pyridoxal kinase activity was increased 2.5-3-fold in stimulated lymphocytes in presence of pyridoxal. Km value was 2.5-5.0.10(-5) M of pyridoxal for preparations of pyridoxal kinase from lymphocytes either with normal or with increased enzymatic activity. Lymphocytes of mice, kept on the diet without pyridoxine within 5-6 weeks, were characterized by distinctly decreased proliferative and cytolytic activities. Pyridoxal-5'-phosphate, added to incubation mixture in vitro, as distinct from pyridoxal, restored partially the impaired functions of T-lymphocytes.
D000818:Animals; D002455:Cell Division; D002478:Cells, Cultured; D003602:Cytotoxicity, Immunologic; D005260:Female; D066298:In Vitro Techniques; D007519:Isoantigens; D007739:L Cells / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D011732:Pyridoxal Phosphate / Q000096:biosynthesis*; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*; D013997:Time Factors; D026681:Vitamin B 6 Deficiency / Q000276:immunology / Q000378:metabolism
D007519:Isoantigens; D011732:Pyridoxal Phosphate
Biosintez piridoksal'-5'-fosfata v limfotsitakh, stimulirovannykh alloantigenami in vitro.