Polyamines and protein synthesis: studies in various polyamine-requiring mutants of Escherichia coli.
Different Escherichia coli mutants auxotrophic for polyamines were studied in order to investigate the relationships among polypeptide synthesis in cell-free systems, ribosomal distribution profiles and endogenous polyamine pools. The in vitro protein synthetic activity and the polyribosomal content were reduced in extracts from putrescine-starved cells of the double mutans MA 255 and MA 261, but not in the arginine-conditional auxotroph DK 6. Putrescine addition to the cultures of all these strains previously starved for polyamines, provoked a shift towards monomers in the equilibrium involving ribosomal particles. Concomitant changes in the intracellular levels of polyamines were observed: putrescine and spermidine increased markedly, and cadaverine disappeared.
D001426:Bacterial Proteins / Q000096:biosynthesis*; D002455:Cell Division; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*; D009154:Mutation; D014176:Protein Biosynthesis / Q000187:drug effects*; D011700:Putrescine / Q000378:metabolism / Q000494:pharmacology*; D012270:Ribosomes / Q000378:metabolism; D013045:Species Specificity; D013997:Time Factors
D001426:Bacterial Proteins; D011700:Putrescine