Differentiation of lymphoid cells: evidence for a B-cell specific serum suppressor.
The induction of immunoglobulin production by rabbit spleen cells is markedly inhibited by the presence of normal rabbit serum during cell culture. A similar inhibition is observed when spleen cell populations in which T cells have been inactivated are temporarily incubated with normal rabbit serum before being reconstituted with T cells by adding thymocytes. In contrast, no inhibition was observed upon temporary incubation of thymocytes with normal serum prior to addition of T cell-inactivated spleen cell populations. Removal of adherent cells did not affect the induction of immunoglobulin production or its inhibition by normal serum. Lipopolysaccharide-enhanced immunoglobin production was also inhibited by normal serum, thereby providing additional confidence that bone-marrow derived (B) cells are the target of the normal serum inhibitor.
D000818:Animals; D000921:Antibody-Producing Cells; D000961:Antilymphocyte Serum / Q000494:pharmacology; D001402:B-Lymphocytes / Q000276:immunology*; D001769:Blood; D007136:Immunoglobulins / Q000096:biosynthesis; D007165:Immunosuppression Therapy; D011817:Rabbits; D013601:T-Lymphocytes / Q000276:immunology
D000961:Antilymphocyte Serum; D007136:Immunoglobulins