Effects of ribosomal mutations on the read-through of a chain termination signal: studies on the synthesis of bacteriophage lambda O gene protein in vitro.
In a DNA-dependent protein-synthesizing system that contains streptomycin-sensitive ribosomes, lambda DNA directs the synthesis of two proteins that are products of the O gene. The larger is produced as a result of read-through of a UGA termination codon. In the system containing streptomycin-resistant ribosomes this read-through protein is not synthesized, indicating that the mutational alteration in the ribosomal protein S12 restricts the read-through. The mutant ribosomes also fail to synthesize the read-through coat protein of RNA phage Qbeta. In addition, the mutant ribosomes restrict suppression of amber mutations in vitro, similar to their effect in vivo.
D003090:Coliphages / Q000378:metabolism*; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000096:biosynthesis; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism; D005796:Genes; D008970:Molecular Weight; D009154:Mutation; D014176:Protein Biosynthesis; D012270:Ribosomes / Q000187:drug effects / Q000502:physiology*; D013307:Streptomycin / Q000494:pharmacology; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000096:biosynthesis*
D004279:DNA, Viral; D014764:Viral Proteins; D004262:DNA Restriction Enzymes; D013307:Streptomycin