title: The effect of rumen epithelial development on metabolic activities and ketogenesis by the tissue in vitro.
abstract: 1. An investigation was made on oxygen consumption, glucose and lactate uptake and ketogenesis from butyrate by rumen epithelium in vitro from lambs at various stages of development. 2. Oxygen uptake was decreased by about 35% and glucose uptake by about 90% between 2 weeks and 1/2 year of age. 3. The uptake of L-lactate and the utilization of butyrate as a substrate for respiration were increased during epithelial development. 4. The production of D(-)-3-hydroxybutyrate and acetoacetate from butyrate by the epithelium was largely increased between 4 to 10 weeks of age, independently of rumen fermentation. 5. A synergistic effect of glucose on the production of D(-)-3-hydroxybutyrate and on total ketone bodies from butyrate by the epithelium was observed. It accounted to 40-80% over butyrate depending on the stage of epithelial development.
mesh_terms: D020155:3-Hydroxybutyric Acid; D000090:Acetoacetates / Q000096:biosynthesis; D000375:Aging / Q000378:metabolism; D000818:Animals; D002087:Butyrates / Q000378:metabolism; D020148:Butyric Acid; D004848:Epithelium / Q000378:metabolism; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D006885:Hydroxybutyrates / Q000096:biosynthesis; D007657:Ketone Bodies / Q000096:biosynthesis*; D007773:Lactates / Q000378:metabolism; D019344:Lactic Acid; D010101:Oxygen Consumption; D012417:Rumen / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D012756:Sheep
chemical_list: D000090:Acetoacetates; D002087:Butyrates; D006885:Hydroxybutyrates; D007657:Ketone Bodies; D007773:Lactates; D020148:Butyric Acid; D019344:Lactic Acid; C016635:acetoacetic acid; D005947:Glucose; D020155:3-Hydroxybutyric Acid