Structural examination of antitumour, water-soluble glucans from Grifora umbellata by use of four types of glucanase.
Antitumour glucans (GU) from the fungus Grifora umbellata have been subjected to periodate oxidation, Smith degradation, methylation analysis, and treatment with endo-(1 leads to 6)-beta-D-, endo-(1 leads to 3)-beta-D-, and exo-(1 leads to 3)-beta-D-glucanases, and alpha-amylase; the following structural features were revealed. GU-2 contains a backbone involving (1 leads to 6)-beta- and () leads to 3)-beta linkages, and two kinds of branches involving (1 leads to 6)-beta and (1 leads to 4)-alpha linkages. GU-3 has a (1 leads to 3)-beta-linked backbone and branches involving (1 leads to 6)-beta linkages or (1 leads to 4)-alpha and (1 leads to 6)-beta linkages. GU-4 also contains a (1 leads to 3) beta-D-glucan backbone and a small number of (1 leads to 6)-beta-linked branches. Probable structural units of these glucans are proposed.
D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use; D055598:Chemical Phenomena; D002621:Chemistry; D005260:Female; D005658:Fungi / Q000032:analysis*; D005936:Glucans / Q000627:therapeutic use; D006026:Glycoside Hydrolases; D051379:Mice; D012510:Sarcoma 180 / Q000188:drug therapy; D000516:alpha-Amylases
D000970:Antineoplastic Agents; D005936:Glucans; D006026:Glycoside Hydrolases; D000516:alpha-Amylases