title: Proteolysis of procollagen I.
abstract: 1. Digestion of procollagen I which trypsin, pepsin or pronase performed at 20 degrees C causes the release of acidic non-collagenous fragments and hydroxyproline-rich fraction. Enzymatic proteolysis performed at 41 degrees C (above the temperature of denaturation) results in degradation of procollagen I to low-molecular peptides. 2. The hydroxyproline-rich fraction obtained by limited proteolysis of procollagen I with pepsin (at 20 degrees C) contains a material corresponding to alpha and beta subunits of tropocollagen. Reduction of the hydroxyproline-rich fraction released by trypsin or pronase (at 20 degrees C) causes the appearance of polypeptides similar to pro-alpha subunits.
mesh_terms: D008970:Molecular Weight; D010434:Pepsin A / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism*; D011347:Procollagen / Q000378:metabolism*; D011402:Pronase / Q000378:metabolism; D013696:Temperature; D014357:Trypsin / Q000378:metabolism
chemical_list: D011347:Procollagen; D010447:Peptide Hydrolases; D014357:Trypsin; D010434:Pepsin A; D011402:Pronase