Manganese(II) as a paramagnetic probe of the tertiary structure of transfer RNA.
The effect of manganese on both the low field (10--15 ppm) and the high field (o--3 ppm) NMR spectra of unfractionated tRNA and yeast tRNAPhe has been investigated. Trace amounts of Mn2+ cause selective broadening of resonances which are assigned to specific tertiary interactions. The order in which resonances broaden is the same as the order in which they are stabilized by the addition of magnesium, namely s4U8 - A14, U33 and A58 - T54. From this we conclude that three of the strong binding sites probably are the same for both Mn2+ and Mg2+, and that these sites are located close to the tertiary interactions which are stabilized by the strongly bound metals. The broadening data, taken in conjunction with published X-ray data on yeast tRNAPhe, permit us to suggest some plausible locations for the strong binding sites.
D001665:Binding Sites; D004926:Escherichia coli; D009682:Magnetic Resonance Spectroscopy; D008345:Manganese / Q000494:pharmacology*; D008958:Models, Molecular; D009690:Nucleic Acid Conformation / Q000187:drug effects*; D010649:Phenylalanine; D012329:RNA, Bacterial; D012343:RNA, Transfer; D015003:Yeasts
D012329:RNA, Bacterial; D008345:Manganese; D010649:Phenylalanine; D012343:RNA, Transfer