title: Studies on gene control regions. 1. Chemical synthesis of lactose operator deoxyribonucleic acid segments.
abstract: The chemical synthesis of lactose operator DNA segments is described. The 31-base-paired duplex contains the DNA recognized by lac repressor protein and twofold rotationally symmetric base pairs on either side of the tight binding region. The synthesis includes the deoxyoligonucleotides d(T-G-T-G-G), d(A-A-T-T-G-T-G-A-G), d(C-G-G-A-T-A-A-C-A-A-T-T), d(T-C-A-C-A), d(T-G-T-G-A-A-A-T-T-G-T), d(T-A-T-C-C-G-C-T-C-A-C), and d(A-A-T-T-C-C-A-C-A). These deoxyoligonucleotides were characterized by two-dimensional sequencing techniques, paper chromatography, and thin-layer chromatography.
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000138:chemical synthesis*; D004926:Escherichia coli; D005815:Genetic Code; D007785:Lactose; D009838:Oligodeoxyribonucleotides; D009876:Operon; D011953:Receptors, Cyclic AMP
chemical_list: D009838:Oligodeoxyribonucleotides; D011953:Receptors, Cyclic AMP; D004247:DNA; D007785:Lactose