title: Induction of chick embryonic intestinal disaccharidases by hydrocortisone and sucrose in the organ culture system.
abstract: The effect of hydrocortisone and sucrose on the development of chick intestinal disaccharidases was studied using the organ culture system. When intestines of 15- and 17-day-old embryos were cultured in the presence of hydrocortisone, there was significant enhancement of disaccharidases activity compared with the control. However, there was no effect in the 20-day embryonic intestines. On the other hand, the disaccharidase activity of cultured intestines from 20-day-old chick embryos were significantly stimulated by the addition of sucrose. The observed increase in disaccharidase activity induced by the administration of hydrocortisone in 17-day-old embryos in vitro was sensitive to actinomycin D and cycloheximide. The activity induced by the administration of sucrose in 20-day-old embryos in vitro was sensitive to cycloheximide but insensitive to actinomycin D.
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002642:Chick Embryo; D003513:Cycloheximide / Q000494:pharmacology; D003609:Dactinomycin / Q000494:pharmacology; D004186:Disaccharidases / Q000096:biosynthesis*; D004790:Enzyme Induction; D006854:Hydrocortisone / Q000494:pharmacology*; D007422:Intestines / Q000187:drug effects / Q000196:embryology / Q000201:enzymology*; D009924:Organ Culture Techniques; D013393:Sucrase / Q000378:metabolism; D013395:Sucrose / Q000494:pharmacology*; D000520:alpha-Glucosidases / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D003609:Dactinomycin; D013395:Sucrose; D003513:Cycloheximide; D004186:Disaccharidases; D000520:alpha-Glucosidases; D013393:Sucrase; D006854:Hydrocortisone