PPD-induced immunoglobulin production in human peripheral blood lymphocytes. II. Separation of PPD-reactive helper T cells from PWM-reactive helper T cells in polyclonal immunoglobulin production of human peripheral blood lymphocytes.
We investigated the relationship bewteen PPD-reactive helper T cells and PWM-reactive helper T cells in polyclonal Ig production of human PBL. Elimination of PPD-reactive T cells by BUdR + light treatment resulted in a loss of helper function in PPD-induced Ig production, but had no effect on helper function in PWM-induced Ig production. On the other hand, elimination of PWM-reactive T cells resulted in a loss of helper function in both PPD-induced Ig production and PWM-induced Ig production. A blast cell-enriched fraction that was generated by PPD and separated by the velocity sedimentation method contained helper function in both responses. On the other hand, blast cell-depleted fractions did not contain PPD-reactive helper function, although the PWM-reactive helper function was evident. These results strongly suggest that PPD-reactive helper T cells are included in PWM-reactive helper T cells.
D001973:Bromodeoxyuridine / Q000378:metabolism; D002469:Cell Separation; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis*; D008027:Light; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D011043:Pokeweed Mitogens / Q000494:pharmacology*; D013601:T-Lymphocytes / Q000276:immunology*; D014373:Tuberculin / Q000494:pharmacology*
D007136:Immunoglobulins; D011043:Pokeweed Mitogens; D014373:Tuberculin; D001973:Bromodeoxyuridine