[Electrophoretic separation of rat spleen and thymus leukocytes and their reactions to mitogens in vitro].
Electrophoretic mobility (EPM) of lymphocytes from the thymus and spleen of August and Wistar rats as well as capacity of lymphocytes with different surface hemagglutinin (PHA) and concanavalin A (Con A) were studied by the method of free flow electrophoresis. Lymphocytes of the rat spleen were shown, depending on the surface charge, to divide into two groups during cultivation: cells with high and low electrophoretic mobility. At separation the lymphocytes consisted of 8--10 fractions with different EPM. There was a relationship between the surface charge of the lymphocytes and their stimulation rate by mitogens. Increased thymidine-3H uptake was recorded at mitogenic exposure of lymphocytes from the spleen with high EPM. Low mobile lymphoid elements of the spleen did not respond to mitogenic stimulation. A subpopulation of thymocytes with low EPM was resistant to Con A stimulation. The thymocytes of rats did not virtually respond to PHA irrespective of EPM.
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology*; D003208:Concanavalin A; D004247:DNA / Q000096:biosynthesis; D004586:Electrophoresis / Q000379:methods; D037102:Lectins; D008213:Lymphocyte Activation; D008297:Male; D051381:Rats; D013601:T-Lymphocytes / Q000166:cytology*; D013936:Thymidine / Q000378:metabolism
D037102:Lectins; D003208:Concanavalin A; D004247:DNA; D013936:Thymidine
Elektroforeticheskoe razdelenie limfotsitov selezenki i timusa krys i ikh reaktsiia na mitogeny in vitro.