In vitro system that synthesizes circular viral DNA of bacteriophage phi X 174.
An extract prepared from Escherichia coli cells infected with phi chi 174 bacteriophage was capable of incorporating dTTP into phage-specific DNAs in vitro. The synthesized DNAs were associated with proteins and sedimented with S values of 20, 50, and 90 in a sucrose gradient sedimentation. DNA isolated from 20S material was open circular replicative form (RF), DNA in 50S material was replicative-form DNA with an extended single-stranded viral DNA that ranged up to one genome in length, and DNA in 90S material consisted of circular and linear single-stranded viral DNA of full genome length and single-stranded viral DNA shorter than full genome length. Pulse and pulse-chase experiments indicated that 90S material derived from 50S material.
D002474:Cell-Free System; D003090:Coliphages / Q000378:metabolism*; D004270:DNA, Circular / Q000096:biosynthesis*; D004277:DNA, Single-Stranded / Q000096:biosynthesis*; D004279:DNA, Viral / Q000096:biosynthesis*; D004926:Escherichia coli; D008954:Models, Biological; D013942:Thymine Nucleotides / Q000378:metabolism
D004270:DNA, Circular; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D013942:Thymine Nucleotides