Identification of a membrane protein associated with expression of the surface exclusion region of the F transfer operon.
Membrane preparations from radioactively labeled male and female strains of Escherichia coli K-12 were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiography. An intensely labeled band corresponding to a protein of molecular weight of 24,000 was readily apparent in preparations from Hfr and F-prime strains but not in those from female strains. When preparations from a series of Hfr strains containing transfer operon deletions were examined, presence of the band was found to be associated with retention of the region of the F transfer operon between ilzA and traD. Thus, the band ("protein S") appears to be the product of an F tra operon activity corresponding to traS (the gene for surface or entry exclusion), or an unknown gene in its vicinity. As predicted, protein S was subject to Fin+ control; only a faint band was detectable if the repressed plasmid R100 was also present in the F lac strain. A 24,000-dalton protein was also found in membrane preparations from strains carrying the derepressed plasmids R100-1 and R1-19 but not in those from strains carrying the repressed plasmids R100 or R1. Thus, the appearance of protein S in the membrane may be a general phenomenon resulting from transfer operon expression of F-like plasmids.
D001426:Bacterial Proteins / Q000096:biosynthesis*; D003227:Conjugation, Genetic; D004926:Escherichia coli / Q000378:metabolism*; D005144:F Factor; D008565:Membrane Proteins / Q000096:biosynthesis*; D008970:Molecular Weight; D009154:Mutation; D009876:Operon; D011815:R Factors
D001426:Bacterial Proteins; D008565:Membrane Proteins