In vivo studies on the cellular source of migration inhibitory factor in mice with delayed hypersensitivity.
Mice sensitized intravenously with 300 microgram of BCG cell walls in Drakeol-Tween 80 and challenged intravenously 3 weeks later with 50 mg of old tuberculin released migration inhibitory factor (MIF) into the circulating blood in quantities that could be detected in serum dilutions of 1:64 to 1:128. When thymus-derived lymphocytes (T-cells) were absent at the time of sensitization, as in neonatally thymectomized mice or in athymic nude mice, detectable amounts of MIF were not formed. Sensitized mice treated with either anti-theta serum or anti-bone marrow-derived lymphocyte (B-cell) serum before intravenous challenge with old tuberculin released reduced amounts of MIF into the circulation. Mice lethally irradiated, reconstituted with B-cells, sensitized with BCG cell walls, and then challenged intravenously 3 weeks later with old tuberculin did not release MIF into the circulation. When T-cells were injected at least 10 days before challenge, however, MIF appeared.
D000818:Animals; D000831:Animals, Newborn; D000961:Antilymphocyte Serum / Q000494:pharmacology; D001402:B-Lymphocytes / Q000276:immunology; D005260:Female; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D007111:Immunity, Cellular; D008263:Macrophage Migration-Inhibitory Factors / Q000096:biosynthesis*; D051379:Mice; D008806:Mice, Inbred AKR; D008809:Mice, Inbred C3H; D008819:Mice, Nude; D011828:Radiation Chimera; D013601:T-Lymphocytes / Q000276:immunology; D013934:Thymectomy; D013997:Time Factors
D000961:Antilymphocyte Serum; D008263:Macrophage Migration-Inhibitory Factors