Some problems in the correction for intrinsic light-scattering of samples in specific protein analysis by automated immunoprecipitation.
Heparinised plasma samples have falsely high blank values in the automated immunoprecipitation system when blanks are measured by omitting antiserum from the assay. This is probably due to an interaction of heparin and serum proteins, together with either fibrinogen or polyethylene glycol. A more accurate estimate of the blank of heparinised plasma samples is obtained by measuring it with a solution of nonimmune serum substituted for the antiserum.
D001769:Blood; D001798:Blood Proteins / Q000032:analysis* / Q000276:immunology; D011232:Chemical Precipitation; D003176:Complement C3 / Q000032:analysis; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D007158:Immunologic Techniques; D008027:Light; D009391:Nephelometry and Turbidimetry / Q000379:methods; D011092:Polyethylene Glycols; D012542:Scattering, Radiation
D001798:Blood Proteins; D003176:Complement C3; D007070:Immunoglobulin A; D007075:Immunoglobulin M; D011092:Polyethylene Glycols; D006493:Heparin