Observations on the morphology at the transition between the peripheral and the central nervous system in the cat. I. A preparative procedure useful for electron microscopy of the lumbosacral dorsal.
A preparatory method, considered to preserve the ultrastructure of the transitional region of lumbosacral dorsal roots in a "useful" way has been tested out. The method consists of vascular perfusion with 5% glutaraldehyde dissolyed in a 300 mOsm Millonig buffer containing 200 mOsm sucrose and 2.7% low molecular dextran, a postfixation in the same medium, a rinse overnight in a normoton phosphate buffer containing 200 mOsm sucrose, an asmication in 2% OsO4 dissolved in a phosphate buffer, an extensive stepwise dehydration starting in 10% acetone and, finally, embedding in Vestopal W.
D000818:Animals; D002415:Cats; D006652:Histological Techniques; D008854:Microscopy, Electron; D010477:Perfusion; D013126:Spinal Nerve Roots / Q000648:ultrastructure*; D014021:Tissue Preservation / Q000379:methods*