Separation of cells with different histocompatibility (H-2) antigens by passage through cellular immunoadsorbent columns.
The anti-Ig column technique of Wigzell et al. (Scand. J. Immunol. 1972. 1:75) was adapted to the separation of mouse cells with different H-2 antigens. Fractionation was achieved by a two-step procedure: first the cells were treated with anti-H-2 sera, and thereafter passed over anti-mouse Ig columns. Antibody-coated cells were selectively retained in the anti-Ig column. The method could be applied to the separation of normal mouse lymphocytes or lymphoma cells with different H-2 antigen complexes and for the selective isolation of H-2 loss variants from a mouse carcinoma/fibroblast hybrid.
D000818:Animals; D002469:Cell Separation; D002471:Cell Transformation, Neoplastic; D003601:Cytotoxicity Tests, Immunologic; D014644:Genetic Variation; D006649:Histocompatibility Antigens; D007163:Immunosorbent Techniques; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009374:Neoplasms, Experimental / Q000276:immunology; D011817:Rabbits
D006649:Histocompatibility Antigens