The elution profile of vitamin D2 metabolites from sephadex LH20 columns.
A metabolite of vitamin D2 in the tissues of primates fed this vitamin co-migrates to a significant extent with 24,25-dihydroxyvitamin-D3 on columns of Sephadex LH20, with chloroform/n-hexane (60/40 by vol) as eluent. In individuals receiving vitamin D2 and in individuals with scant exposure to sunlight this metabolite, which we believe to be 25,26-dihydroxyvitamin-D2, may yield falsely higher concentrations of apparent 24,25-dihydroxyvitamin-D3 if separation on LH20 is used.
D000818:Animals; D001842:Bone and Bones / Q000378:metabolism; D002112:Calcifediol; D002250:Carbon Radioisotopes; D002428:Cebus; D004100:Dihydroxycholecalciferols / Q000302:isolation & purification; D004872:Ergocalciferols / Q000031:analogs & derivatives / Q000302:isolation & purification / Q000378:metabolism*; D006887:Hydroxycholecalciferols / Q000302:isolation & purification; D007413:Intestinal Mucosa / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D009132:Muscles / Q000378:metabolism; D014316:Tritium
D002250:Carbon Radioisotopes; D004100:Dihydroxycholecalciferols; D004872:Ergocalciferols; D006887:Hydroxycholecalciferols; D014316:Tritium; C028140:24,25-dihydroxyvitamin D2; D002112:Calcifediol