title: Methionyl-transfer ribonucleic acid deficiency during G1 arrest of Saccharomyces cerevisiae.
abstract: The mesl- mutants of Saccharomyces cerevisiae cease division and accumulate in the G1 interval of the cell cycle when deprived of methionine or shifted from 23 to 36 degrees C in the presence of methionine. Synchronous cell cycle arrest results from a deficiency of charged methionyl-transfer ribonucleic acid (methionyl-tRNAMet) as shown by direct measurement of the in vivo pools of methionine, S-adenosylmethionine, and methionyl-tRNAMet. The deficiency of methionyl-tRNAMet in these cells is the consequence of a lesion in a single gene, mes1. mes1 appears to be the structural gene for the methionyl-tRNA synthetase because some revertants of this mutation exhibited a thermolabile methionyl-tRNA synthetase in vitro. A sufficient hypothesis to explain these and previous results is that the control of cell division by S. cerevisiae in response to nutrient limitation is mediated through aminoacyl-tRNA or subsequent steps in protein biosynthesis.
mesh_terms: D000604:Amino Acyl-tRNA Synthetases / Q000096:biosynthesis*; D002455:Cell Division; D003433:Crosses, Genetic; D005796:Genes; D008715:Methionine / Q000378:metabolism; D008718:Methionine-tRNA Ligase / Q000096:biosynthesis*; D009154:Mutation; D010641:Phenotype; D012343:RNA, Transfer / Q000378:metabolism*; D012436:S-Adenosylmethionine / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism*; D013696:Temperature
chemical_list: D012436:S-Adenosylmethionine; D012343:RNA, Transfer; D008715:Methionine; D000604:Amino Acyl-tRNA Synthetases; D008718:Methionine-tRNA Ligase