Studies on the substrate specificity of Taka-amylase A. XIII. Preparation of 6-deoxy-6-iodomaltooligosaccharides and their inhibitory action against Taka-amylase A1.
O-alpha-D-Glucopyranosyl-(1 leads to 4)-O-6-deoxy-6-iodo-alpha-D-glucopyranosyl-(1 leads to 4)-D-glucopyranose (6'-MT), O-alpha-D-glucopyranosyl-(1 leads to 4)-6-deoxy-6-iodo-D-glucopyranose (6-M), and O-6-deoxy-6-iodo-alpha-D-glucopyranosyl-(1 leads to 4)-D-glucopyranose (6'-M) were prepared and their inhibitory action against Taka-amylase A [EC 3.2.1.1, alpha-1, 4-glucan 4-glucanohydrolase, Aspergillus oryzae] was investigated. The inhibitor constants of 6'-MT and 6'-M were 10 mM and 54 mM, respectively, and both inhibitors showed mixed-type inhibition. 6-M scarcely inhibited the enzyme action.
D000681:Amylases / Q000037:antagonists & inhibitors*; D001230:Aspergillus / Q000201:enzymology*; D001236:Aspergillus oryzae / Q000201:enzymology*; D001665:Binding Sites; D004187:Disaccharides / Q000494:pharmacology; D007455:Iodine; D008320:Maltose / Q000031:analogs & derivatives; D009844:Oligosaccharides / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D013379:Substrate Specificity; D014312:Trisaccharides / Q000494:pharmacology; D000516:alpha-Amylases / Q000037:antagonists & inhibitors*
D004187:Disaccharides; D009844:Oligosaccharides; D014312:Trisaccharides; D008320:Maltose; D007455:Iodine; D000681:Amylases; D000516:alpha-Amylases