Improved methods for separation and purification by affinity chromatography.
Defined conditions for simplified Affinity Chromatography suitable for instructional and research purposes, were developed. The standard method of activation of polysaccharide gels using BrCN was modified and improved using a highly buffered reaction solution. A batchwise protocol for the immunoadsorbent isolation of anti-BSA was used (with commonly employed dissociation agents) and comparative data were obtained.
D000097:Acetonitriles; D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods; D003488:Cyanogen Bromide; D006041:Goats; D007163:Immunosorbent Techniques; D012710:Serum Albumin, Bovine / Q000276:immunology
D000097:Acetonitriles; D000906:Antibodies; D012710:Serum Albumin, Bovine; D003488:Cyanogen Bromide