Inhibition of Clostridium botulinum types A and B hemagglutinins by sugars.
Chromatographically isolated hemagglutinins of Clostridium botulinum types A and B are serologically related but not identical. Of the sugars (5, 6, 12, 18 carbons, some derivatives, L  and D forms) tested, only D-galactose and some of tis derivatives were inhibitors of these hemagglutinins. O-Nitrophenyl-beta-D-galactopyranoside and isopropyl-beta-D-thiogalactoside were the most potent inhibitors. The two hemagglutinins were bound tightly by p-aminophenyl-beta-D-thiogalactopyranoside coupled to CH-Sepharose 4B. The ligands to which these hemagglutinins bind were determined as the sugars which inhibited the hemagglutinating activity.
D000373:Agglutinins; D002241:Carbohydrates / Q000494:pharmacology*; D003014:Clostridium botulinum / Q000276:immunology*; D005690:Galactose / Q000494:pharmacology; D006384:Hemagglutination / Q000187:drug effects*; D006388:Hemagglutinins; D007544:Isopropyl Thiogalactoside / Q000494:pharmacology; D009598:Nitrophenylgalactosides / Q000494:pharmacology; D013045:Species Specificity; D013237:Stereoisomerism
D000373:Agglutinins; D002241:Carbohydrates; D006388:Hemagglutinins; D009598:Nitrophenylgalactosides; D007544:Isopropyl Thiogalactoside; D005690:Galactose