Quantification of cell death in developing cerebellum by a 14C tracer method.
To study the question of whether or not cell death contributes significantly to normal or stressed postnatal brain development in a way which is biochemically quantifiable, we carried out an experiment to assess the amount of cell death in developing cerebellum. By measuring the loss of DNA content and the loss of 14C from labelled thymidine previously incorporated into the DNA fraction (DNAF) in X-irradiated neonatal animals, shown by histological methods to have cell death to the degree of degranulating the external granular layer (EGL), we showed that when cells die both label and DNA content are greatly decreased in the cerebellum. Experiments on both normal and malnourished animals showed that cell death does not contribute significantly to cerebellar development in either malnutrition-stressed or normal animals. Here, we present a biochemical tool for assessing cell death and evidence that cell death does not contribute significantly to cerebellar development.
D000818:Animals; D000831:Animals, Newborn; D002250:Carbon Radioisotopes; D002470:Cell Survival; D002531:Cerebellum / Q000032:analysis / Q000166:cytology / Q000254:growth & development / Q000502:physiology* / Q000503:physiopathology; D004247:DNA / Q000032:analysis / Q000528:radiation effects; D009748:Nutrition Disorders / Q000503:physiopathology; D051381:Rats
D002250:Carbon Radioisotopes; D004247:DNA