[Interaction of the isolated DNA of lambda phage with spheroplasts of E. coli treated with sturine].
The method of centrifugation in sucrose density gradient (30-55%) of the spheroplast membrane preparations treated and untreated with sturine and infected with phage lambda DNA demonstrated that sturine, treatment increased the phage lambda DNA absorption three-fold. About 50% of the lambda DNA molecules adsorbed by spheroplasts are bound with the cytoplasmic membrane of spheroplasts treated with sturine; 50% of the lambda DNA molecules are bound with the cell wall membrane on the sturine-untreated spheroplasts. The data obtained allow to conclude that the stimulating effect of sturine in E. coli spheroplasts transfection by lambda DNA is connected with redistribution of phage DNA absorbed on spheroplasts from the cell wall to the cytoplasmic membrane facilitating the penetration of DNA and its fastening on the membrane.
D003090:Coliphages; D004279:DNA, Viral; D004926:Escherichia coli / Q000187:drug effects*; D011506:Proteins / Q000494:pharmacology*; D013104:Spheroplasts; D014170:Transformation, Genetic / Q000187:drug effects*
D004279:DNA, Viral; D011506:Proteins
O vzaimodeĭstvii izolirovannoĭ DNK faga lambda so sferoplastami E. coli, obrabotannymi sturinom.