Surface charge of trypanosoma cruzi. Binding of cationized ferritin and measurement of cellular electrophoretic mobility.
The surface charge of epimastigote and trypomastigote forms of Trypanosoma cruzi was evaluated by means of binding of cationized ferritin to the cell surface as visualized by electron microscopy, and by direct measurements of the cellular microelectrophoretic mobility (EPM). Epimastigote forms had a mean EPM of -0.52 micrometer-s-1-V-1-cm and were lightly labeled with cationized ferritin. In contrast, bloodstream trypomastigotes had a much higher EPM (-1.14), and the surface was heavily labeled with cationized ferritin. When trypomastigotes from staionary phase cultures were isolated on DEAE cellulose columns, the mean EPM was found to be significantly lower (-0.63), and labeling with cationized ferritin decreased. With a mixed population containing epimastigote, trypomastigote, and intermediate forms, EPM values ranging between -0.70 to -1.14 were found. From these observations we conclude that there is a definite increase in negative surface charge during development from epi- to trypomastigote forms of T. cruzi.
D000818:Animals; D001665:Binding Sites; D001769:Blood / Q000469:parasitology; D002412:Cations; D004586:Electrophoresis; D005293:Ferritins / Q000378:metabolism; D051379:Mice; D008854:Microscopy, Electron; D013499:Surface Properties; D014345:Trypanosoma / Q000378:metabolism; D014349:Trypanosoma cruzi / Q000254:growth & development / Q000502:physiology*
D002412:Cations; D005293:Ferritins