The immunization of mice and calves with gal E mutants of Salmonella typhimurium.
A galactose epimeraseless (gal E) mutant of Salmonella typhimurium was investigated in mice and calves for its suitability as a live vaccine. In mice, a very highly significant difference in the mortality rates was observed when vaccinated and non-vaccinated animals were challenged with virulent strains of S. typhimurium and S. dublin. In calves, doses of 10(6) and above of gal E mutant injected subcutaneously provided highly significant protection both in terms of mortality and prevalence of symptoms when calves were challenged orally with S. typhimurium. However, there appeared to be a relation between the vaccine and the presence of renal lesions and before gal E mutants can be recommended, further work is necessary to determine the pathogenesis of these lesions.
D000818:Animals; D001428:Bacterial Vaccines / Q000009:adverse effects; D002238:Carbohydrate Epimerases / Q000378:metabolism*; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control; D005069:Evaluation Studies as Topic; D005260:Female; D005690:Galactose; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D009154:Mutation; D012480:Salmonella Infections / Q000517:prevention & control*; D012481:Salmonella Infections, Animal / Q000517:prevention & control; D012486:Salmonella typhimurium / Q000201:enzymology*; D014611:Vaccination / Q000662:veterinary
D001428:Bacterial Vaccines; D002238:Carbohydrate Epimerases; D005690:Galactose