Synthetic disaccharide-protein antigens for production of specific 04 and 09 antisera for immunofluorescence diagnosis of salmonella.
The synthetic disaccharides abequose 1 leads to a 3 mannose and tyvelose 1 leads to a 3 mannose, representative of Salmonella O-antigen 4 and 9 respectively, were covalently linked to bovine serum albumin (BSA) . Antisera from rabbits immunized with these immunogens were used in indirect immunofluorescence assay for the identification of group B (O-antigen 4) and D (O-antigen 9) Salmonella. A total of 1030 enteric bacterial strains were tested, including 207 group B and 55 group D Salmonella. The anti-abequose-mannose-BSA serum correctly identified all Salmonella group B strains tested. The anti-tyvelose-mannose-BSA serum correctly indentified all Salmonella group D bacteria examined with the exception of 11 of 18 Vi-positive S. typhi strains which did not not stain until the Vi-antigen was removed by boiling. Among the 768 strains representing Salmonella other than groups B and D, E. coli, Shigella, Citrobacter, Klebsiella, Enterobacter, Serratia, Proteus, Vibrio, Pseudomonas, Aeromonas, Yersinia, Bacteroides and Fusobacterium only 5 positive reactions were found. These were observed with Y. pseudotuberculosis strains which have the same disaccharide antigenic determinants as Salmonella O-antigen 4 and 9 respectively. The high specificity of the antisera elicited by the synthetic disaccharide-BSA immunogens make them suitable for a specific and rapid identification of Salmonella bacteria belonging to serogroups B and D.
D000818:Animals; D000918:Antibody Specificity; D002417:Cattle; D004755:Enterobacteriaceae / Q000276:immunology; D005243:Feces / Q000382:microbiology; D005455:Fluorescent Antibody Technique; D007106:Immune Sera / Q000032:analysis*; D008358:Mannose / Q000276:immunology*; D011817:Rabbits; D012475:Salmonella / Q000276:immunology; D012480:Salmonella Infections / Q000175:diagnosis*; D012703:Serotyping; D012710:Serum Albumin, Bovine / Q000276:immunology*
D007106:Immune Sera; D012710:Serum Albumin, Bovine; D008358:Mannose