Use of antibody-coated red cells for the sensitive detection of antigen and in rosette tests for cells bearing surface immunoglobulins.
Conditions for an improved chromic chloride method for the attachment of antibody to red cells are described. The method, which is applicable to whole Ig fractions as well as affinity-purified antibodies, is reproducible and has a sensitivity for detection of antigen in the nanogram range. The coated cells may be used in a rosette assay for the detection of cell surface-bound antigens.
D000906:Antibodies; D000941:Antigens / Q000032:analysis*; D002857:Chromium; D004912:Erythrocytes / Q000276:immunology*; D007158:Immunologic Techniques; D066298:In Vitro Techniques; D008832:Microchemistry; D011947:Receptors, Antigen, B-Cell / Q000032:analysis*
D000906:Antibodies; D000941:Antigens; D011947:Receptors, Antigen, B-Cell; D002857:Chromium