title: Modification of permeability of frog perineurium to [14C]-sucrose by stretch and hypertonicity.
abstract: An in vitro method has been developed to determine quantitatively the permeability of the perineurium to radiotracers at room temperature. The permeability to [14C]sucrose of the isolated perineurium of the sciatic nerve of the frog, Rana pipiens, was measured at rest length, when the perineurium was stretched and after the perineurium had been subjected to hypertonic treatment. Mean permeability at rest length was calculated to be 5.6 +/- 0.27 (S.E.M., n = 45) X 10(-7) cm/sec, and both stretch and hypertonic treatment increased the permeability. A 10% stretch increased permeability reversibly, whereas a 20% stretch or immersion of the perineurium in a hypertonic bath increased permeability irreversibly. Altered permeability under these conditions might be related to changes in the ultrastruct of tight junctions in the perineurium.
mesh_terms: D000818:Animals; D001001:Anura; D002463:Cell Membrane Permeability; D005260:Female; D009994:Osmolar Concentration; D011894:Rana pipiens; D012584:Sciatic Nerve / Q000378:metabolism* / Q000502:physiology; D013314:Stress, Mechanical; D013395:Sucrose / Q000378:metabolism*
chemical_list: D013395:Sucrose