Novel method for estimating the labeling index in clinical specimens with the use of immunoperoxidase-labeled.
The labeling index determined by [3H]thymidine autoradiography in cells from clinical specimens was compared with the percentage of the cells showing nuclear reactivity to immunoperoxidase-labeled antinucleoside antibodies. This nuclear immunoreactivity is specific for denatured or single-stranded DNA's and is detectable almost exclusively during DNA synthesis. Results with the two methods showed excellent agreement. The new method allowed rapid accurate assessment of S phase in the tumor cells from freshly isolated aspirated specimens as well as frozen sections, suggesting general applicability to estimation of the labeling index without autoradiography.
D000230:Adenocarcinoma / Q000378:metabolism; D000818:Animals; D002455:Cell Division; D004273:DNA, Neoplasm / Q000378:metabolism*; D005260:Female; D005629:Frozen Sections; D006801:Humans; D007124:Immunoenzyme Techniques; D008325:Mammary Neoplasms, Experimental / Q000378:metabolism; D051379:Mice; D008809:Mice, Inbred C3H; D009369:Neoplasms / Q000378:metabolism / Q000473:pathology*; D009705:Nucleosides / Q000276:immunology; D013936:Thymidine / Q000378:metabolism
D004273:DNA, Neoplasm; D009705:Nucleosides; D013936:Thymidine