Use of omega-aminohexyl-sepharose in the fractionation of Escherichia coli B aminoacyl-tRNA synthetases.
The usefulness of aminohexyl-Sepharose in purification of E. coli B aminoacyl-tRNA synthetases is presented. The purification factors for 14 synthetases lie in the range 3- to 94-fold and the recoveries of the enzymatic activity were 30-80%, depending on the enzyme.
D000604:Amino Acyl-tRNA Synthetases / Q000302:isolation & purification*; D004926:Escherichia coli / Q000201:enzymology*; D011134:Polysaccharides / Q000031:analogs & derivatives; D012685:Sepharose / Q000031:analogs & derivatives
D011134:Polysaccharides; D012685:Sepharose; D000604:Amino Acyl-tRNA Synthetases