Inhibition of vitamin A action in rat bone cultures by inhibitors of RNA and protein synthesis.
Using explanted humeri of late fetal rats, retinoic acid induced a dose- and time-dependent regression of cartilage. The retinoic acid-induced release of proteoglycan into the medium was blocked by inhibitors of RNA and protein synthesis, suggesting its dependence upon continuous RNA and protein synthesis.
D000546:Amanitins / Q000494:pharmacology; D001862:Bone Resorption / Q000187:drug effects; D001842:Bone and Bones / Q000187:drug effects* / Q000378:metabolism; D003513:Cycloheximide / Q000494:pharmacology; D004247:DNA / Q000378:metabolism; D003609:Dactinomycin / Q000494:pharmacology; D003839:Deoxyadenosines / Q000494:pharmacology; D009924:Organ Culture Techniques; D011506:Proteins / Q000378:metabolism; D011509:Proteoglycans / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects; D014212:Tretinoin / Q000037:antagonists & inhibitors*; D014801:Vitamin A / Q000031:analogs & derivatives*
D000546:Amanitins; D003839:Deoxyadenosines; D011506:Proteins; D011509:Proteoglycans; D014801:Vitamin A; D003609:Dactinomycin; D014212:Tretinoin; D012313:RNA; D004247:DNA; D003513:Cycloheximide