Consecutive gas chromatographic determination of phenytoin, phenobarbital, primidone, phenylethylmalondiamide, carbamazepine, trimethadione, dimethadione, ethosuximide, and valproate from the same serum specimen.
A quantitative gas-liquid chromatographic procedure is described for the consecutive determination of phenytoin, phenobarbital, primidone, phenylethylmalondiamide, carbamazepine, trimethadione, dimethadione, ethosuximide and valproate from a single serum specimen of 1.2 ml. After extraction from serum by two different procedures, the anticonvulsants are chromatographed without further purification on a 3% OV 17 column either with or without derivative formation by means of "on-column" methylation. Multiple internal standards are employed in order to enhance the reproducibility of drug-concentration measurement.
D000927:Anticonvulsants / Q000097:blood*; D002220:Carbamazepine / Q000097:blood; D002849:Chromatography, Gas / Q000379:methods*; D004114:Dimethadione / Q000097:blood; D005013:Ethosuximide / Q000097:blood; D010634:Phenobarbital / Q000097:blood; D010657:Phenylethylmalonamide / Q000097:blood; D010672:Phenytoin / Q000097:blood; D011324:Primidone / Q000097:blood; D014293:Trimethadione / Q000097:blood; D014635:Valproic Acid / Q000097:blood
D000927:Anticonvulsants; D011324:Primidone; D002220:Carbamazepine; D005013:Ethosuximide; D014635:Valproic Acid; D010672:Phenytoin; D010657:Phenylethylmalonamide; D004114:Dimethadione; D014293:Trimethadione; D010634:Phenobarbital