The aminoacyladenylate mechanism in the aminoacylation reaction of yeast phenylalanyl-tRNA synthetase.
It is shown from a combination of rapid quenching and steady-state kinetics that the phenylalanyl-tRNA synthetase from yeast catalyses the formation of phenylalanyl-tRNA by the amino-acyladenylate pathway at pH 7.8 and 25 degrees C. The rate-determining step at saturating reagent concentrations is not the dissociation of the charged tRNA from the enzyme.
D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism*; D007700:Kinetics; D010649:Phenylalanine; D010652:Phenylalanine-tRNA Ligase / Q000378:metabolism*; D012346:RNA, Transfer, Amino Acyl; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
D012346:RNA, Transfer, Amino Acyl; D010649:Phenylalanine; D000604:Amino Acyl-tRNA Synthetases; D010652:Phenylalanine-tRNA Ligase