Effect of lipopolysaccharide on the development of cell-mediated immunity to transplantation antigens.
Lipopolysaccharide (LPS) from Escherichia coli administered to allografted mice in a single dose of 20 microgram influenced the development of cell-mediated immunity to skin donor antigens. As an indicator of cell-mediated immunity, donor antigen-induced inhibition of cell migration from spleen explants of allografted animals was used. Cultures of spleen explants were established 16 days after grafting. The antigen-specific inhibition of migration was abolished when LPS was injected either 4 to 2 days before grafting and intensified when LPS was administered on the 2nd day after grafting.
D000818:Animals; D005260:Female; D006085:Graft Survival; D006649:Histocompatibility Antigens / Q000276:immunology*; D007111:Immunity, Cellular; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008263:Macrophage Migration-Inhibitory Factors / Q000096:biosynthesis; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016038:Skin Transplantation; D014184:Transplantation, Homologous
D006649:Histocompatibility Antigens; D008070:Lipopolysaccharides; D008263:Macrophage Migration-Inhibitory Factors