Purification and characterization of granulocytic progenitor cells (CFU-C) from human peripheral blood using immunologic surface markers.
The concentration of committed granulocytic progenitor cells (CFU-C) in functionally unique subpopulations of human peripheral blood mononuclear cells has been determined by the in vitro methyl-cellulose assay. Using immunoabsorbent column chromatography and rosette-depletion techniques, we have demonstrated that CFU-C, although not present in either purified T or B lymphocyte populations, are highly concentrated in the "null" cell population, which lacks sheep erythrocyte receptors and surface immunoglobulin. Further fractionation of this null subset has demonstrated that CFU-C do not bear complement receptors, but require the presence of peripheral blood mononuclear cell feeder layers for maximum proliferation.
D001854:Bone Marrow Cells; D002469:Cell Separation; D002478:Cells, Cultured; D003470:Culture Media; D006098:Granulocytes; D006410:Hematopoiesis; D006801:Humans; D007163:Immunosorbent Techniques; D007962:Leukocytes; D008212:Lymphocyte Depletion; D011947:Receptors, Antigen, B-Cell; D012397:Rosette Formation
D003470:Culture Media; D011947:Receptors, Antigen, B-Cell