Codon-specific interaction of uncharged transfer-RNA with eukaryotic ribosomes.
Rat liver ribosomes bound [32P]tRNAPhe in both a codon-dependent and codon-independent manner. The codon-dependent binding was studied further by utilising the ability of the unchanged tRNAPhe to inhibit the poly(U)-directed binding of [3H]Phe-tRNA to ribosomes. At least part of the codon-dependent binding of uncharged tRNA appears to be to the ribosomal A-site.
D001665:Binding Sites; D003062:Codon / Q000378:metabolism*; D004926:Escherichia coli; D008099:Liver; D008274:Magnesium; D010649:Phenylalanine; D011072:Poly U / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*
D003062:Codon; D012333:RNA, Messenger; D011072:Poly U; D010649:Phenylalanine; D012343:RNA, Transfer; D008274:Magnesium