A new semiquantitative radiometric opsonin assay. Selective measurement of opsonizing capacity of the alternative pathway.
A new semiquantitative radiometric opsonin assay is described. It was found that the opsonin activity generated by incubating brewer's yeast, Saccharomyces cerevisiae, in medium containing less than 5% human serum was exclusively complement dependent. In contrast, C. albicans was effectively opsonized in the absence of complement. Antibodies and the early classical complement pathway did not contribute to the opsonization of S. cerevisiae and neither did C5-9. The brewer's yeast assay can therefore be used for measuring selectively the opsonizing capacity of the alternative pathway. Sera from approximately 7% of apparently healthy adult controls consistently failed to generate significant opsonin activity while 8 out of 26 patients with suspected immune deficiency of unknown cause were defective in this assay. All opsonin deficient sera so far tested had haemolytically normal alternative pathway and Factor B activity.
D000328:Adult; D002176:Candida albicans / Q000276:immunology; D003167:Complement Activation; D003170:Complement Pathway, Alternative; D006801:Humans; D007153:Immunologic Deficiency Syndromes / Q000276:immunology; D008722:Methods; D009504:Neutrophils / Q000276:immunology; D009895:Opsonin Proteins / Q000032:analysis*; D010587:Phagocytosis; D012016:Reference Values; D012441:Saccharomyces cerevisiae / Q000276:immunology
D009895:Opsonin Proteins