Sandwich enzymoimmunoassay of hepatitis B surface antigen (HBsAg).
A sandwich enzymoimmunoassay (EIA) procedure was developed for the detection of HBsAg using Fab' of anti-HBsAg conjugated with beta-D-galactosidase from Escherichia coli with anti-HBsAg-coated silicone rubber discs as a solid phase. EIA could detect 1 ng/ml of HBsAg. It was as sensitive as radioimmunoassay (RIA, AusRia II) and about 30-fold more sensitive than reversed passive hemagglutination assay RPHA, ReverseCell). EIA and RIA could detect more HBsAg-positive sera than RPHA.
D005696:Galactosidases; D006386:Hemagglutination Tests; D006509:Hepatitis B / Q000276:immunology; D006514:Hepatitis B Surface Antigens; D006801:Humans; D007124:Immunoenzyme Techniques; D011863:Radioimmunoassay
D006514:Hepatitis B Surface Antigens; D005696:Galactosidases