Purification of human monocytes by continuous gradient sedimentation in ficoll.
A new method of obtaining purified human monocytes has been developed. The peripheral blood mononuclear leukocytes are isolated by centrifugation over Ficoll--Hypaque and then further purified by sedimentation over a linear 5--10% Ficoll density gradient. In ten experiments, the average purity obtained was 77.1% macrophages and the mean yield was 22.4% of the monocytes contained in the peripheral blood leukocytes. Viability of monocytes isolated by this technique exceeded 95%. The cells were phagocytic and responded to human migration inhibitory factor.
D001772:Blood Cell Count; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D005362:Ficoll / Q000494:pharmacology*; D006801:Humans; D008263:Macrophage Migration-Inhibitory Factors / Q000494:pharmacology; D009000:Monocytes / Q000502:physiology; D011134:Polysaccharides / Q000494:pharmacology*
D008263:Macrophage Migration-Inhibitory Factors; D011134:Polysaccharides; D005362:Ficoll