Effect of rat intestinal glycoprotein on complement lytic activity of 32P-labelled Escherichia coli.
Evidence for complement activation by an intestinal glycoprotein fraction from germfree rats is presented. The lowering effect on human serum bactericidal activity of E. coli was 28% for the intestinal glycoprotein fraction, 31% for zymosan, and 25% for inulin, while the lowering effect on conventional rat serum bactericidal activity was 65%, 53% and 21%, respectively. 32P-releasing activity of serum from germfree rats and from man on labelled E. coli amounted to 8% and 28%, respectively, of the release exerted by serum from conventional rats.
D000818:Animals; D000907:Antibodies, Bacterial; D001770:Blood Bactericidal Activity; D002473:Cell Wall / Q000187:drug effects; D003165:Complement System Proteins / Q000378:metabolism; D004926:Escherichia coli / Q000276:immunology*; D006023:Glycoproteins / Q000494:pharmacology*; D007123:Immunoelectrophoresis, Two-Dimensional; D007422:Intestines / Q000276:immunology; D007444:Inulin / Q000494:pharmacology; D051381:Rats; D015054:Zymosan / Q000494:pharmacology
D000907:Antibodies, Bacterial; D006023:Glycoproteins; D007444:Inulin; D003165:Complement System Proteins; D015054:Zymosan