title: Periplasmic localization of nicotinate phosphoribosyltransferase in Escherichia coli.
abstract: Nicotinate phosphoribosyltransferase (NAPRTase) in Escherichia coli mediates the formation of nicotinate mononucleotide, a direct precursor of nicotinamide adenine dinucleotide (NAD), from nicotinate and 5-phosphoribosyl-1-pyrophosphate. Specifically, NAPRTase contributes to NAD synthesis by utilizing intracellular nicotinate formed from NAD degradation products, which are recycled by NAD cycle enzymes and exogenous nicotinate when it is available. In previous studies, it has been tacitly assumed that almost all NAD cycle enzymes are localized in the cytoplasm of E. coli. The results of this investigation provide evidence that NAPRTase is a periplasmic (extracytoplasmic) enzyme. The osmotic shock of exponential-phase cells of E. coli K-12 and ML 308-225 resulted in the release of 63 to 72% and 42 to 48%, respectively, of the NAPRTase into the shock medium. In addition, when exponential cells of strains K-12 and ML 308-225 were converted into spheroplasts, 75 to 84% and 54 to 68%, respectively, of the enzyme was released into the spheroplast medium. Since previous estimates of the effective levels of NAPRTase present in putative repressed and derepressed E. coli cells appeared to be very low, a more convenient and accurate alternative method for the evaluation of NAPRTase in whole cells was developed. The results show that NAPRTase is subject only to a modest degree of enzyme repression. In addition, no evidence was found for the presence of a protein or low-molecular-weight inhibitor of the enzyme in repressed cells.
mesh_terms: D003593:Cytoplasm / Q000201:enzymology; D004794:Enzyme Repression; D004926:Escherichia coli / Q000201:enzymology*; D009539:Nicotinic Acids / Q000494:pharmacology; D009997:Osmotic Pressure; D010430:Pentosyltransferases / Q000032:analysis* / Q000096:biosynthesis; D013104:Spheroplasts / Q000201:enzymology
chemical_list: D009539:Nicotinic Acids; D010430:Pentosyltransferases; C021864:nicotinate phosphoribosyltransferase