Localisation of e-antigen in nuclei of hepatocytes in HBsAg-positive liver diseases.
By the direct immunofluorescence technique 19 liver biopsies were examined for the presence of the e-antigen associated with the hepatitis B virus infection. With the use of double incubation and blocking experiments with FITC and Rhodamine labelled antisera against hepatitis B core antigen and e-antigen, evidence is presented that the e-antigen is localised in the nuclei of the hepatocytes. We conclude that the e-antigen and the core antigen are not identical, although they are often present simultaneously in the nucleus of the hepatocyte.
D002467:Cell Nucleus / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006509:Hepatitis B / Q000276:immunology*; D006511:Hepatitis B Antigens / Q000032:analysis*; D006512:Hepatitis B Core Antigens / Q000032:analysis; D006514:Hepatitis B Surface Antigens / Q000032:analysis; D006801:Humans; D008099:Liver / Q000276:immunology* / Q000648:ultrastructure
D006511:Hepatitis B Antigens; D006512:Hepatitis B Core Antigens; D006514:Hepatitis B Surface Antigens