Ninhydrin inhibition of glucose-induced insulin release.
Ninhydrin, a compound which shares chemical properties strikingly similar to alloxan was found to mimic basically the inhibitory effect of alloxan on glucose-induced insulin release. Exposure of pancreatic islets for five minutes to 85 mumol/l ninhydrin produced approximately ninety percent inhibition of subsequent glucose-induced insulin release without altering basal secretion. Both D-glucose and D-mannose provided substantial protection against the inhibitory effect of ninhydrin, and the alpha anomer of D-glucose was more effective than the beta anomer in preventing ninhydrin inhibition of insulin release. Evidence for a common site of inhibition by ninhydrin and alloxan in the insulin release process is discussed.
D000818:Animals; D005947:Glucose / Q000494:pharmacology*; D006601:Hexoses / Q000494:pharmacology; D066298:In Vitro Techniques; D007192:Indenes / Q000494:pharmacology*; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009555:Ninhydrin / Q000494:pharmacology*; D051381:Rats
D006601:Hexoses; D007192:Indenes; D007328:Insulin; D009555:Ninhydrin; D005947:Glucose