Enrichment for the globin coding region in a chromatin fraction from chick reticulocytes by endonuclease digestion.
A nuclease-sensitive fraction was obtained from chick reticulocyte chromatin by brief digestion with an endonuclease (DNAase II, deoxyribonucleate 3'-oligonucleotidohydrolase, EC 3.1.4.6). The nuclease-sensitive fraction typically contained less than 1% of the chromatin-DNA but about 50% or more of the nascent chromatin-bound RNA. Hybridization of chick globin complementary DNA to the DNA component of the nuclease-sensitive fraction of reticulocyte chromatin indicated a 3--5 fold enrichment for the globin coding region of the chromatin. The control experiment utilizing DNA from a nuclease-sensitive fraction of chick liver chromatin did not show a comparable enrichment for the globin coding region. This suggests that the endonuclease-effected enrichment for the globin coding region in the nuclease-sensitive fraction of reticulocyte chromatin is to some degree specific for structural genes transcribed in reticulocytes.
D000818:Animals; D002645:Chickens; D002843:Chromatin / Q000235:genetics*; D004247:DNA / Q000032:analysis / Q000235:genetics; D004262:DNA Restriction Enzymes / Q000378:metabolism; D005796:Genes; D005821:Genetic Techniques; D005914:Globins / Q000235:genetics*; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D012333:RNA, Messenger / Q000235:genetics; D012156:Reticulocytes / Q000648:ultrastructure; D014158:Transcription, Genetic
D002843:Chromatin; D012333:RNA, Messenger; D005914:Globins; D004247:DNA; D004262:DNA Restriction Enzymes