Arrangement of protein I in Escherichia coli outer membrane: cross-linking study.
The arrangement of protein I in the outer membrane of Escherichia coli was investigated by cross-linking whole cells, isolated cell wall, protein-peptidoglycan complexes, and protein I released from peptidoglycan with NaCl. Both cleavable azide cross-linkers and imidoester reagents were used. The data presented suggest that protein I exists in the outer membrane as a trimer.
D001386:Azides; D001426:Bacterial Proteins; D002473:Cell Wall; D004120:Dimethyl Suberimidate; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000648:ultrastructure*; D008565:Membrane Proteins; D008970:Molecular Weight; D010457:Peptidoglycan; D011487:Protein Conformation
D001386:Azides; D001426:Bacterial Proteins; D008565:Membrane Proteins; D010457:Peptidoglycan; D004120:Dimethyl Suberimidate