[Levels of biogenic amines and their metabolites in rat whole brain after rapid tissue fixation with microwave irradiation (author's transl)].
Levels of norepinephrine (NE), dopamine (DA), serotonin (5-HT), 3, 4-dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA) and 5-hydroxy-indoleacetic acid were measured fluorometrically in the whole brain of rats killed either by decapitation or by 5kW microwave irradiation for 1.6 sec. which inactivates the relevant brain enzymes rapidly and irreversibly. There were statistically no differences in the levels of NE, DA, 5-HT and 5-HIAA between the two methods of sacrifice, while the level of DA increased slightly in irradiated brains. On the other hand, the level of DOPAC, an oxidative deaminated metabolite of DA, increased significantly and the level of HVA, a final metabolite of DA, reduced markedly in the irradiated brains compared to that in the decapitated brains, respectively. These findings suggest that the turnover rates for metabolism of DA at synaptic nerve terminals and synaptic clefts may be relatively rapid. Therefore, it may be concluded that rapid inactivation of the brain enzymes involved in metabolism of DA is necessary prior to analysis of DA and its metabolites and microwave irradiation is the most suitable method available at the present time.
D000818:Animals; D001679:Biogenic Amines / Q000378:metabolism*; D001921:Brain / Q000378:metabolism* / Q000528:radiation effects; D004298:Dopamine / Q000378:metabolism; D006652:Histological Techniques; D008297:Male; D008872:Microwaves; D009638:Norepinephrine / Q000378:metabolism; D051381:Rats; D012701:Serotonin / Q000378:metabolism
D001679:Biogenic Amines; D012701:Serotonin; D004298:Dopamine; D009638:Norepinephrine