Assessment of whole blood for antibody-dependent cell-mediated cytotoxicity in kidney patients.
A test using whole blood for antibody-dependent cell-mediated cytotoxicity has been assessed in kidney patients. The antibody-coated target system comprised lymphocytes isolated from a normal donor sensitised with rabbit anti-human lymphocyte serum. Comparison of results obtained with whole blood and isolated lymphocytes from the same patients shows that the whole blood test amplifies the differences between normal subjects, dialysis and transplant patients. It probably combines the effects of depressed lymphocyte function and lymphopenia and thus represent a more physiological in vitro correlate for in vivo immune function.
D000906:Antibodies / Q000032:analysis; D000920:Antibody-Dependent Cell Cytotoxicity; D001379:Azathioprine / Q000627:therapeutic use; D003601:Cytotoxicity Tests, Immunologic; D006084:Graft Rejection; D006801:Humans; D007141:Immunoglobulin Fc Fragments; D016030:Kidney Transplantation; D007694:Killer Cells, Natural / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology; D008775:Methylprednisolone / Q000627:therapeutic use; D011239:Prednisolone / Q000627:therapeutic use; D006435:Renal Dialysis; D014184:Transplantation, Homologous; D014511:Uremia / Q000628:therapy
D000906:Antibodies; D007141:Immunoglobulin Fc Fragments; D011239:Prednisolone; D001379:Azathioprine; D008775:Methylprednisolone