Dissociability of free and peptidyl-tRNA bound ribosomes.
The influence of peptidyl-tRNA on the dissociation of yeast 80 S ribosomes into subunits was studied. For this purpose temperature-sensitive (ts) suppressor strain of yeast Saccharomyces cervisiae carrying a defect in peptide chain termination was used. It was found that peptidyl-tRNA did not influence the dissociation of ribosomes either at high salt concentration or in the presence of dissociation factor (DF) from yeast. After dissociation of yeast ribosomes in 0.5 M KCl, peptidyl-tRNA remains bound to the 60 S subunit. Some characteristics of the termination process and release of nascent polypeptides from yeast ribosomes are discussed.
D009154:Mutation; D010443:Peptide Chain Termination, Translational; D010455:Peptides / Q000378:metabolism*; D011189:Potassium Chloride / Q000008:administration & dosage; D012343:RNA, Transfer / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism* / Q000648:ultrastructure; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D013696:Temperature
D010455:Peptides; D011189:Potassium Chloride; D012343:RNA, Transfer