title: The effects of trimethoprim and erythromycin on polysome metabolism in Escherichia coli.
abstract: Trimethoprim and erythromycin were shown to have different overall effects on in vivo polysome metabolism in Escherichia coli. In a rel A+-rel A pair of strains, trimethoprim treatment induces a reduction of polysome level to a variable extent, similarly to aminoacyl tRNA deprivation of cells, but persisting polysomes remain dynamic structures in a state of continual turnover. In contrast, erythromycin stabilizes polysome level to a high value in either kind of strain, but maintained polysomes appear as "frozen" structures unable to undergo ribosome translocation.
mesh_terms: D004917:Erythromycin / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects*; D011132:Polyribosomes / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism; D014295:Trimethoprim / Q000494:pharmacology*
chemical_list: D004917:Erythromycin; D012343:RNA, Transfer; D014295:Trimethoprim