Polyclonal activation of human B lymphocytes in vitro by pokeweed mitogen: a simple technique for the simultaneous assessment of cell proliferation, generation of plasma cells, plaque-forming cells and immunoglobulin production.
A simple technique has been worked out for the simultaneous assessment of cell proliferation, generation of plasma cells, plaque-forming cells and immunoglobulin production from single cultures of 1 X 10(6) human peripheral blood lymphocytes stimulated with pokeweed mitogen (PWM). Kinetic studies showed that number of cells, thymidine incorporation rate, number of plaque-forming cells against fluorescein-isothiocyanate-haptenated sheep erythrocytes and concentration of IgM and IgG in the supernatant peaked on the average between day 5 and 7 of culture. This technique is particularly suitable for the analysis of hypogammaglobulinaemias in infancy.
D000328:Adult; D001402:B-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D001772:Blood Cell Count; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D006462:Hemolytic Plaque Technique; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis*; D007158:Immunologic Techniques; D007958:Leukocyte Count; D008213:Lymphocyte Activation; D010950:Plasma Cells / Q000276:immunology; D011043:Pokeweed Mitogens / Q000494:pharmacology*
D007136:Immunoglobulins; D011043:Pokeweed Mitogens; D004247:DNA