Comparison of three methods of differentiating bovine mycoplasma.
Growth inhibition (GI) by specific antisera, direct fluorescent antibody technique (FAT), and fatty-acid profile analysis by gas-liquid chromatography (GLC) were compared as methods for identifying 13 strains of bovine mycoplasma. By the FAT, there were 8 bilateral and 13 unilateral cross reactions. In the GI study, there were 4 bilateral and 7 unilateral cross reactions. In both FAT and GI studies, there were fluorescence and inhibition, respectively, with 13 homologous antisera. Analysis by GLC revealed the 13 mycoplasmas could be placed into 4 distinct chromatographic groupings. The GLC profiles of 2 organisms were sufficiently unique that they could be used for specific identification.
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis; D002417:Cattle / Q000382:microbiology; D002849:Chromatography, Gas; D005227:Fatty Acids / Q000032:analysis; D005455:Fluorescent Antibody Technique; D009174:Mycoplasma / Q000032:analysis / Q000145:classification* / Q000276:immunology
D000942:Antigens, Bacterial; D005227:Fatty Acids