[The agar culture of the leucopoietic stem cell (CFU-C) and its stimulation (author's transl)].
This review summarizes the different in vitro culture techniques of the leukopoietic stem cell. Mainly the results obtained by the system of Pluznik and Sachs and Bradley and Metcalf are described apply with semisolid agar and its modification by Iscove using methylcellulose. Furthermore, recently published modifications of culture technique are listed. The principal results obtained with mouse and human bone marrow cells are summarized with respect to the origin of the colony-stimulating factor acting in different species, its biochemical characteristics, as well as with regard to the colony forming cell and its different subclasses.
D000818:Animals; D003114:Colony-Forming Units Assay; D003115:Colony-Stimulating Factors; D003470:Culture Media; D006098:Granulocytes; D006801:Humans; D007962:Leukocytes; D008722:Methods; D051379:Mice; D009000:Monocytes
D003115:Colony-Stimulating Factors; D003470:Culture Media
Die in vitro-Kultur der leukopoetischen Stammzelle und ihre Stimulation.