Nephelometric assay of antigens and antibodies with latex particles.
Antigens and antibodies covalently bound to latex particles have been used to detect specifically corresponding antibodies and antigens by nephelometry. A systematic study of factors such as wavelength, angle of observation, concentration of latex particles, and reaction time has permitted us to develop a procedure suitable for routine estimation of antigens and antibodies in the nano and picogramme range.
D000906:Antibodies / Q000032:analysis*; D000918:Antibody Specificity; D000941:Antigens / Q000032:analysis*; D007158:Immunologic Techniques; D007840:Latex; D008027:Light; D008832:Microchemistry; D008863:Microspheres; D010783:Photometry; D012542:Scattering, Radiation; D013997:Time Factors
D000906:Antibodies; D000941:Antigens; D007840:Latex