[Albumin mRNA from rat liver cells. Analysis of immunoadsorption of individual polyribosomes].
The analysis of albumin polyribosomes immunoadsorption is carried out using "sandwich" immunoadsorbents prepared on the basis of two aminobenzylcelluloses: commercial (paraaminobenzylcellulose) and synthesised (methaaminobenzyloxymethylcellulose). A method is worked out which is good for the estimation of the adaptibility of different aminobenzylcellulose preparations as an insoluble basis for the immunoadsorbent. Major properties of the "sandwich" sorbent (the accessible capacity and specificity) and the percent of isolated individual polyribosomes are found to be interrelated and determined by conditions of the immunoadsorption reaction. The increase of polyribosomes and sorbent concentrations and their ratio in the incubation medium results in the increase of the sorbent accessible capacity and the decrease in the inspecific adsorption but at the same time the percent of adsorbed polyribosomes decrease too. The "excess" of adsorbent with respect to polyribosomes, participating in the binding reaction, is necessary for the quantitative isolation of individual polyribosomes.
D000418:Albumins; D000818:Animals; D007163:Immunosorbent Techniques; D007164:Immunosorbents; D008099:Liver / Q000166:cytology*; D011132:Polyribosomes; D012333:RNA, Messenger; D051381:Rats
D000418:Albumins; D007164:Immunosorbents; D012333:RNA, Messenger
Al'buminovaia mRNK iz kletok pecheni krysy. Analiz protsessa immunosorbtsii individual'nykh poliribosom.