title: Osmotic imbalance in inositol-starved spheroplasts of Saccharomyces cerevisiae.
abstract: Physiological states associated with inositol starvation of spheroplasts of Saccharomyces cerevisiae were investigated and compared with conditions preceding death of starved whole cells. In the absence of synthesis of inositol-containing lipids, cell surface expansion terminated after one doubling of whole cells. In spheroplasts, cessation of membrane expansion was apparently followed by rapid development of an osmotic imbalance, causing lysis. Continued synthesis and accumulation of cytoplasmic constituents within the limited cell volume were implicated as a cause of the osmotic imbalance. In whole cells, an increase in internal osmotic pressure also follows termination of membrane and cell wall expansion. The cell wall prevents lysis, allowing a state of increasing cytoplasmic osmotic pressure to persist in the period preceding onset of inositol-less death.
mesh_terms: D003513:Cycloheximide / Q000494:pharmacology; D005656:Fungal Proteins / Q000096:biosynthesis; D007294:Inositol / Q000378:metabolism*; D009996:Osmotic Fragility / Q000187:drug effects; D011188:Potassium / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D013104:Spheroplasts / Q000378:metabolism / Q000502:physiology*
chemical_list: D005656:Fungal Proteins; D007294:Inositol; D003513:Cycloheximide; D011188:Potassium