[Transplantation of somatic nuclei into activated teleost eggs (the example of the loach, Misgurnus fossilis L.)].
Some peculiarities of the technique of nuclear transplantation for teleostean fishes are described, the loach (Misgurnus fossilis) taken as an example. The cells of the late high blastula were used as donors and the activated non-enucleated and enucleated (by X-rays, 20 kR) eggs of the loach as recipients. Following the nuclear transplantation in the activated non-enucleated eggs, 33 (out of 128) normal blastulae were obtained, one of which developed until hatching. Following the nuclear transplantation in the activated enucleated eggs, 34 (out of 251) blastulae were obtained, 6 embryos hatched and 2 larvae attained the stage of active feeding.
D000818:Animals; D002455:Cell Division / Q000528:radiation effects; D002999:Clone Cells / Q000166:cytology; D003584:Cytological Techniques; D005260:Female; D005399:Fishes / Q000196:embryology* / Q000254:growth & development; D007814:Larva / Q000254:growth & development; D053652:Nuclear Transfer Techniques; D010063:Ovum / Q000166:cytology* / Q000528:radiation effects; D013997:Time Factors
Peresadka somaticheskikh iader v aktivirovannye iaĭtsa kostistykh ryb (na primere v'iuna Misgurnus fossilis L.).