Solid-phase radioimmunoassay for detection of antibodies to myelin basic protein.
A solid-phase double-antibody radioimmunoassay was developed for the detection of anti-myelin basic protein (BP) in sera. Antigen was adsorbed to glass test tubes, reacted with rat anti-BP sera, followed by 125I-labeled rabbit anti-rat IgG. This assay was capable of detection of specific antibody at low nanogram per ml levels, was technically simple, and the results correlated well with established procedures.
D000818:Animals; D000906:Antibodies; D005260:Female; D006168:Guinea Pigs; D007106:Immune Sera / Q000494:pharmacology; D007122:Immunoelectrophoresis; D007163:Immunosorbent Techniques; D009185:Myelin Proteins / Q000276:immunology*; D010446:Peptide Fragments / Q000276:immunology; D011817:Rabbits; D011863:Radioimmunoassay; D051381:Rats; D011917:Rats, Inbred Lew; D013997:Time Factors
D000906:Antibodies; D007106:Immune Sera; D009185:Myelin Proteins; D010446:Peptide Fragments