[Analysis of the thermostability of the hybrid DNA molecules of microorganisms as a means of increasing the resolution of the molecular hybridization technic].
A comparative study of termostability of microorganisms DNA was performed in order to increase the resolution of the method of molecular hybridization. Molecular hybridization was carried out and the curve of hybrid DNA duplexes distribution, acccording to termostability of two groups of microorganisms, related to strains Echerichia coli B1 and Vibrio cholerae eltor 18647, were obtained. It was determined that the form of the curves is specie specific for the microorganisms investigated but there exists a similarity between the closely related strains which can not be distinguished by the percent of homology.
D001483:Base Sequence; D004269:DNA, Bacterial / Q000032:analysis; D004926:Escherichia coli / Q000032:analysis / Q000235:genetics*; D006358:Hot Temperature; D009693:Nucleic Acid Hybridization; D013045:Species Specificity; D014734:Vibrio cholerae / Q000032:analysis / Q000235:genetics*
D004269:DNA, Bacterial
Analiz termostabil'nosti gibridnykh molekul DNK mikroorganizmov kak sredstvo povysheniia razreshaiushcheĭ sposobnosti metoda molekuliarnoĭ gibridizatsii.