Antibody response in cattle and rabbits to early antigens of malignant catarrhal fever virus in cultured cells.
Cytosine arabinoside (Ara-C) inhibited the production of late antigens and of infectious virus in monolayers of bovine kidney cells infected with the high-passage, WC-11 strain of malignant catarrhal fever virus. Early antigens were not affected. Using hyperimmune and acute-phase sera from cattle and rabbits in indirect immunofluorescence tests, it was shown that Ara-C treated cultures contained two early antigens; one was diffuse and distributed throughout the cells, the other was particulate and intranuclear. Antibody to early antigens developed later and attained lower titres in infected animals, especially rabbits; only hyperimmune sera reacted with the diffuse early antigen.
D000818:Animals; D000917:Antibody Formation; D000956:Antigens, Viral / Q000276:immunology*; D002417:Cattle / Q000276:immunology*; D002478:Cells, Cultured; D003561:Cytarabine / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D006564:Herpesviridae / Q000276:immunology*; D008304:Malignant Catarrh / Q000382:microbiology*; D011817:Rabbits / Q000276:immunology*
D000956:Antigens, Viral; D003561:Cytarabine