Characterization of pemphigus antigen.
New factors controlling the reaction of pemphigus antibody and the pemphigus antigens were defined by studying the action of antibody on section prepared from human skin. The pemphigus antigenicity was protected by adding dithiothreitol and CaCl2 in buffer. Blocking studies of the -SH groups by sulfhydryl reagents rapidly decreased the reactivity of antigens. In addition pretreatment calcium chelating agents also decreased this reactivity.
D000328:Adult; D000941:Antigens / Q000032:analysis*; D001666:Binding Sites, Antibody; D001667:Binding, Competitive; D005455:Fluorescent Antibody Technique; D006801:Humans; D010392:Pemphigus / Q000276:immunology*; D012867:Skin / Q000276:immunology
D000941:Antigens