Double-antibody solid-phase enzyme immunoassay for the detection of staphylococcal enterotoxin A.
A simple double-antibody enzyme immunoassay that uses a microtechnique was developed for detecting staphylococcal enterotoxin A in food products. Sample preparation can be completed in less than 15 min. Assay sensitivity ranges from 0.4 ng (20-h test time) to 3.2 ng (1- to 3-h test time) of toxin per ml of prepared sample. Separation and detection of enterotoxin from spiked food products ranged between 72 and 98% of the amount added.
D004768:Enterotoxins / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D007124:Immunoenzyme Techniques; D013210:Staphylococcus
D004768:Enterotoxins