Interaction of DNA with DNA-binding proteins: protein exchange and complex stability.
The competition of the DNA-binding proteins I and II of Escherichia coli and of the phage fd DNA-binding protein for single-stranded DNA was investigated. Their roles in cells might be judged from their binding affinities to DNA and their mutual exchange in the DNA . protein complexes. Strongest binding on single strands was found for the phage protein. DNA-binding protein II displaced half of the protein I in the complex with single-stranded DNA when no double-stranded DNA was present. Protein-complexed single strands were protected against degradation. The protection is less pronounced for protein II which can increase the stability of the fd DNA complex with DNA-binding protein I against nucleolytic cleavage.
D001426:Bacterial Proteins / Q000378:metabolism; D001667:Binding, Competitive; D002352:Carrier Proteins / Q000378:metabolism*; D003090:Coliphages / Q000378:metabolism; D004261:DNA Replication; D004277:DNA, Single-Stranded / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism; D003851:Deoxyribonucleases / Q000494:pharmacology; D004926:Escherichia coli / Q000378:metabolism; D014764:Viral Proteins / Q000378:metabolism
D001426:Bacterial Proteins; D002352:Carrier Proteins; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D014764:Viral Proteins; D003851:Deoxyribonucleases