Transconjugant analysis: limitations on the use of sequence-specific endonucleases for plasmid identification.
We used the sequence-specific endonucleases EcoRI, SmaI, BamHI, HsuI, and HaeIII as identification tools in following the conjugal transfer of the well-studied R plasmids Sa, R388, RP4, and R6K. Transfers were both intergeneric and intrageneric. Plasmid fingerprints were generated from both single- and combination-enzyme digests. The Sa transconjugants yielded plasmids showing consistent fingerprints for each of the respective endonucleases used, whereas the three other R-plasmid transconjugants showed fingerprint changes.
D001483:Base Sequence; D003227:Conjugation, Genetic; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000032:analysis*; D004755:Enterobacteriaceae / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D007709:Klebsiella / Q000235:genetics; D011815:R Factors; D012477:Salmonella enteritidis / Q000235:genetics
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes