An enzyme activity specific for nitrous acid-treated DNA in Escherichia coli.
An enzyme activity specifically active on nitrous acid-treated DNA was found in an extract of Escherichia coli. The enzyme acts on both double- and single-stranded DNAs, treated with nitrous acid, in the presence of EDTA, although the former DNA is a better substrate. Evidence is presented that nitrous acid- and bisulfite-induced types of damage in DNA are recognized by different enzymes: (1) Uracil-DNA glycosylase, purified 250-fold from E. coli 1100, attacks bisulfite-treated DNA but not nitrous acid-treated DNA. (2) Almost equal levels of activity toward nitrous acid-treated DNA were found in wild-type and uracil-DNA glycosylase-deficient strains of E. coli.
D003090:Coliphages; D004260:DNA Repair; D004279:DNA, Viral / Q000378:metabolism*; D003856:Deoxyuracil Nucleotides; D004926:Escherichia coli / Q000201:enzymology*; D009154:Mutation; D009699:N-Glycosyl Hydrolases / Q000378:metabolism; D009573:Nitrites; D009608:Nitrous Acid
D004279:DNA, Viral; D003856:Deoxyuracil Nucleotides; D009573:Nitrites; D009699:N-Glycosyl Hydrolases; D009608:Nitrous Acid