title: The respiratory epithelium. VI. Histogenesis of lung tumors induced by benzo[a]pyrene-ferric oxide in the hamster.
abstract: Lung tumors were induced in female Syrian golden hamsters by intratracheal instillation of benzo[a]pyrene-Fe2O3. The tumors were characterized with the use of coordinated morphologic and histochemical techniques including electron microscopy. The lung carcinomas were classified according to their presumed cell of origin. Most were derived from mucous cells and/or basal cells, and they were classified as either epidermoid carcinomas or as combined epidermoid and adenocarcinomas. The tumors in the second group (57% of the total number of carcinomas) presented a wide spectrum of epidermoid and adeno components. The epidermoid component was characterized in well-differentiated tumors by the presence of intercellular bridges and/or keratinization. Well-developed desmosomes and numerous bundles of tonofilaments were observed ultrastructurally. In diagnosing adenocarcinoma, one no longer needs to depend on the presence of tubules or gross glandular structures as criteria for diagnosis. The presence of intracellular and/or extracellular alveoli, well-developed Golgi complex, and endoplasmic reticulum and/or evidence of mucous secretion provide more definitive criteria. A tumor composed of neurosecretory cells that morphologically resembled a bronchial carcinoid of man was observed. Nests of uniform, small, polygonal cells with round-to-oval nuclei were seen at the light microscopic level. Dense-core secretory granules 1,100-2,200 A were present in the cytoplasm of the tumor cells. Several fibrosarcomas were observed. The tumors showed a very cellular structure, composed of either densely packed ovoid or spindle-shaped cells. Ultrastructurally, the cells resembled fibroblasts. The results obtained in this study give strong support for a histogenetic classification, i.e., a classification based on the cell of origin.
mesh_terms: D000230:Adenocarcinoma / Q000145:classification / Q000209:etiology* / Q000378:metabolism; D000818:Animals; D001580:Benzopyrenes; D002294:Carcinoma, Squamous Cell / Q000145:classification / Q000209:etiology* / Q000378:metabolism; D002454:Cell Differentiation; D006224:Cricetinae; D005260:Female; D005290:Ferric Compounds; D007501:Iron; D008175:Lung Neoplasms / Q000145:classification / Q000209:etiology* / Q000378:metabolism; D008647:Mesocricetus; D008854:Microscopy, Electron; D009093:Mucus / Q000378:metabolism; D009374:Neoplasms, Experimental / Q000209:etiology
chemical_list: D001580:Benzopyrenes; D005290:Ferric Compounds; D007501:Iron