title: [Cytologic and histochemical study of cell cultures chronically infected with fixed rabies virus].
abstract: Cytoplasmic inclusion bodies corresponding in their tinctorial properties to Negri bodies were detected in TEp-2/2 and BHK-21/13S cell cultures chronically infected with fixed rabies virus. The number of cells containing the inclusions was always than that of the cells producing virus-specific antigen. Histological examinations of chronically infected cultures revealed considerable inhibition of the acid phosphatase activity, some weakening of the reaction to alkaline phosphatase, and a marked decline in the activity of succinate dehydrogenase. The intensity of reaction to RNA in chronically infected cultures was increased, particularly in those zones of the cells where RNA-containing inclusions were detected. The activity of the respiratory enzyme HAD-H2 tetrasolium reductase in HEp-2/2 cells was reduced and in BHK-21/13S cells increased as compared to the control.
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000956:Antigens, Viral; D002999:Clone Cells; D005455:Fluorescent Antibody Technique; D006651:Histocytochemistry; D007181:Inclusion Bodies, Viral; D010088:Oxidoreductases / Q000378:metabolism; D011818:Rabies / Q000201:enzymology / Q000473:pathology; D011820:Rabies virus / Q000276:immunology
chemical_list: D000956:Antigens, Viral; D010088:Oxidoreductases; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase
vernacular_title: Tsitologicheskoe i gistokhimicheskoe izuchenie kletochnykh kul'tur, khronicheski infitsirovannykh fiksirovannym virusom beshenstva.