title: Isolation and characterisation of 14-S RNA from spinach chloroplasts.
abstract: 14-S RNA was purified from spinach chloroplasts. It has a molecular weight of 0.43 . 10(6) and the following nucleotide composition: 20% CMP, 23.9% AMP, 24.2% GMP and 31.9% UMP. The accumulation of 14-S RNA in chloroplasts of cotyledons of dark-grown plants is stimulated by light. Conditions are described for the isolation of 14-S RNA in the absence of appreciable fragmentation of chloroplast 23-S rRNA and the evidence that it represents a distinct type of chloroplast RNA is discussed. Translation of 14-S RNA in a protein synthesising system from Escherichia coli gives rise to two polypeptides with molecular weights of 13 200 and 12 600 and the possible role of 14-S RNA as a chloroplast messenger is discussed.
mesh_terms: D002736:Chloroplasts / Q000032:analysis*; D004926:Escherichia coli / Q000378:metabolism; D008970:Molecular Weight; D010944:Plants; D014176:Protein Biosynthesis; D012313:RNA / Q000302:isolation & purification / Q000378:metabolism; D012333:RNA, Messenger / Q000302:isolation & purification / Q000378:metabolism; D012265:Ribonucleotides / Q000032:analysis
chemical_list: D012333:RNA, Messenger; D012265:Ribonucleotides; D012313:RNA