title: Circular dichroism studies of the binding of o-nitrophenyl-beta-D-fucoside and o-nitrophenyl-beta-D-galactoside to lac repressor.
abstract: The binding of o-nitrophenyl-beta-D-fucoside and o-nitrophenyl-beta-D-galactoside to Escherichia coli lac repressor was investigated by circular dichroism in the wavelength range 300--400 nm corresponding to the o-nitrophenyl chromophores. The CD signal of both ligands drastically changed when they bound to lac repressor due to the asymmetric interaction of the o-nitrophenyl ring with chemical groups of protein. The CD spectra of bound ligands indicate close similarity between the environment of o-nitrophenyl-beta-D-fucoside and o-nitrophenyl-beta-D-galactoside on lac repressor. The CD signal is used to calculate the binding parameters (K and n) to lac repressor. It is demonstrated that the limited proteolytic digestion of lac repressor which gives a 'core protein' does not affect the environment of both ligands on the protein.
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D002942:Circular Dichroism; D004794:Enzyme Repression; D004926:Escherichia coli / Q000235:genetics; D005643:Fucose / Q000031:analogs & derivatives*; D006027:Glycosides; D007785:Lactose / Q000378:metabolism; D008024:Ligands; D008968:Molecular Conformation; D009598:Nitrophenylgalactosides; D011485:Protein Binding; D013862:Thiogalactosides / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D006027:Glycosides; D008024:Ligands; D013862:Thiogalactosides; D009598:Nitrophenylgalactosides; D005643:Fucose; D007785:Lactose