Ex-vivo removal of mammalian immunoglobulin G: method and immunological alterations.
We have described a clinically feasible method capable of rapidly and repeatedly removing mammalian IgG extracorporeally by adsorption onto heat-killed, formalin-stabilized Staphylococcus aureus Cowan-I. In dogs, 2-13 grams of IgG were removed in 60-70 minutes, lowering the serum concentration of IgG by 30-70 percent. Serum IgG levels returned to pre-run values within 48-72 hours and, in some cases, were higher than pre-run levels at 48-72 hours. Serum biochemical alterations were generally transient and relatively modest. Peripheral lymphocyte counts showed minimal changes but the percentage of erythrocyte rosette forming cells appeared to increase with successive runs. This method is safe and efficient, and can be repetitively performed at short intervals in dogs. This method may prove useful in diseases in which rapid removal of circulating IgG is desired.
D000818:Animals; D004285:Dogs; D005112:Extracorporeal Circulation; D007074:Immunoglobulin G / Q000302:isolation & purification*; D007163:Immunosorbent Techniques; D008722:Methods; D010956:Plasmapheresis; D013211:Staphylococcus aureus
D007074:Immunoglobulin G