Effects of chronic ethanol treatment on rat liver mitochondrial protein synthesis.
Chronic ethanol treatment of male Sprague-Dawley rats resulted in a 50% decrease in the rate of incorporation of precursor leucine into isolated mitochondria. This decrease is manifest in a decreased labeling of three polypeptides of inner mitochondrial membranes that are the major products of in vitro mitochondrial protein synthesis under the conditions employed. Immunoprecipitation of cytochrome-c oxidase revealed that these three polypeptides are subunits 1, 2, and 3 of cytochrome-c oxidase and have apparent molecular weights of 33,000, 25,000, and 20,000. Sixty percent of the total incorporated radioactivity is associated with these polypeptides. A decrease in the contents of subunit 2 and of a second polypeptide with an apparent molecular weight of 22,000 was also noted as an effect of chronic ethanol treatment.
D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D003579:Cytochrome Reductases / Q000378:metabolism; D003864:Depression, Chemical; D000431:Ethanol / Q000494:pharmacology*; D066298:In Vitro Techniques; D008297:Male; D008566:Membranes / Q000378:metabolism; D008930:Mitochondria, Liver / Q000187:drug effects / Q000378:metabolism*; D014176:Protein Biosynthesis; D051381:Rats; D013997:Time Factors
D000596:Amino Acids; D000431:Ethanol; D003579:Cytochrome Reductases