Experiences with radioimmunoassay in the detection of HBsAg in the presence of anti-HBs.
Interference from anti-HBs present during hepatitis B antigenemia caused the appearance of several false negative reactions in a competition radioimmuno-precipitation procedure. The results indicate that such interference is considerably less in the solid-phase radioimmunoassay (Ausria II) and probably causes few false negative tests. The use of pepsin treatment as a means to destroy interfering antibodies is discussed.
D000914:Antibodies, Viral; D005188:False Negative Reactions; D006509:Hepatitis B / Q000276:immunology*; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens / Q000032:analysis*; D006801:Humans; D010434:Pepsin A / Q000494:pharmacology; D011863:Radioimmunoassay / Q000379:methods; D013210:Staphylococcus / Q000276:immunology
D000914:Antibodies, Viral; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D010434:Pepsin A