[Demonstration of specific serum IgG with an "in vitro" radioimmunoassay (RAST IgG) (author's transl)].
By using discs coated with allergens (RAST, Pharmacia) anti-human gammaglobulin goat antibodies, we could demonstrate specific IgG on sera whose IgE had been destroyed by heating at 56 degrees C for 4 hours. A detailed protocol is given. Preliminary results demonstrate absence of specific IgG in untreated patients with pollen allergy, and conversely presence of these IgG in patients treated with immunotherapy against pollens; most patients with epithelia, food and/or mould allergies had specific IgG prior to immunotherapy.
D003888:Desensitization, Immunologic; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology / Q000628:therapy; D007074:Immunoglobulin G / Q000032:analysis*; D011852:Radioallergosorbent Test
D007074:Immunoglobulin G
Mise en évidence d'IgG sériques spécifiques par une technique isotopique in vitro (RAST-IgG).