Characterization of a xenogeneic antiserum raised against the fetal germ cells of the mouse: cross-reactivity with embryonal carcinoma cells.
A xenogeneic antiserum (PG-1) raised against the germ cells of the 13.5d p.c. mouse conceptus reacts with the fetal germ cells of both sexes and adult male germ cells, but not with any nongerminal tissue that has been tested. PG-1 can also react with the EC cells of four different teratocarcinoma cell lines. There are, however, marked differences in the absorptive capacity of the different EC cell lines. The antigen(s) recognized by the antiserum are confined to only a proportion of cells in some EC lines. This antiserum has a number of potential applications in studies of the origin and development of the mouse germ cell lineage.
D000818:Animals; D000918:Antibody Specificity; D002460:Cell Line; D003429:Cross Reactions; D003602:Cytotoxicity, Immunologic; D005455:Fluorescent Antibody Technique; D005854:Germ Cells / Q000276:immunology*; D051379:Mice; D013724:Teratoma / Q000276:immunology*