Studies by radioiodination of normal adult, fetal and leukemic cell membranes.
A comparison was made between cord blood lymphocytes, normal adult lymphocytes and leukemic cells after membrane iodination with lactoperoxidase. A double-labeling technique using lactoperoxidase iodination with 125I and 131I followed by analysis on polyacrylamide gel electrophoresis revealed a number of membrane differences between leukemic, normal and fetal cells. There was a reduction in the 70,000 molecular weight component in cord blood cells compared to adult lymphocytes, and an increase in membrane peptides with molecular weights of 35,000, 20,000, 9,000 and 4,000. Although smaller molecular weight peptides were also present in chronic lymphatic leukemia as well as acute myeloid leukemia, these were shown to be distinct from fetal type membrane components.
D000951:Antigens, Neoplasm / Q000032:analysis; D000954:Antigens, Surface / Q000032:analysis; D002462:Cell Membrane / Q000032:analysis* / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D005312:Fetal Blood / Q000032:analysis; D006801:Humans; D007124:Immunoenzyme Techniques; D007231:Infant, Newborn; D007457:Iodine Radioisotopes; D007945:Leukemia, Lymphoid / Q000097:blood*; D015470:Leukemia, Myeloid, Acute / Q000097:blood*; D008214:Lymphocytes / Q000032:analysis* / Q000276:immunology; D008565:Membrane Proteins / Q000032:analysis / Q000097:blood*; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis / Q000097:blood*
D000951:Antigens, Neoplasm; D000954:Antigens, Surface; D007457:Iodine Radioisotopes; D008565:Membrane Proteins; D010455:Peptides