Acid precipitation of Clostridium botulinum type C and D toxins from whole culture by addition of ribonucleic acid as a precipitation aid.
The ratios of ribonucleic acid to protein contents of Clostridium botulinum type C, D, and E cultures were lower than those of type A, B, and F cultures. Addition of ribonucleic acid at 0.4 mg/ml to culture satisfactorily aided acid precipitation of type C and D toxins, but not that of type E toxin.
D001426:Bacterial Proteins / Q000032:analysis; D001905:Botulinum Toxins / Q000032:analysis / Q000302:isolation & purification*; D011232:Chemical Precipitation; D003014:Clostridium botulinum / Q000032:analysis; D012313:RNA; D012329:RNA, Bacterial / Q000032:analysis; D012441:Saccharomyces cerevisiae
D001426:Bacterial Proteins; D012329:RNA, Bacterial; D012313:RNA; D001905:Botulinum Toxins