A rho-dependent termination site in the gene coding for tyrosine tRNA su3 of Escherichia coli.
A set of partially overlapping DNA restriction fragments that support promoter-dependent transcription of the tRNATyr1 gene of Escherichia coli has been used to study site-specific termination in vitro. Transcription termination occurs at a specific site 224-226 nucleotides beyond the end of the structural gene and is completely dependent on rho-factor. Certain features of this site suggest differences from other termination sites previously studied. A role for specific sequence recognition is suggested.
D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D009694:Nucleic Acid Precursors / Q000235:genetics*; D012329:RNA, Bacterial / Q000235:genetics; D012343:RNA, Transfer / Q000235:genetics*; D012234:Rho Factor / Q000235:genetics*; D013489:Suppression, Genetic; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic; D014443:Tyrosine
D004269:DNA, Bacterial; D009694:Nucleic Acid Precursors; D012329:RNA, Bacterial; D012234:Rho Factor; D014157:Transcription Factors; D014443:Tyrosine; D012343:RNA, Transfer; D004262:DNA Restriction Enzymes