Phagocytosis of 32P-labelled E. coli by human polymorphonuclear cells (PMN). Adaptation of a method.
A system for the study of phagocytosis by human polymorphonuclear cells (PMN) is presented. The leucocytes are harvested from heparinized whole blood by the Bøyum method and transferred to glass tubes to yield glass adherent monolayers of leucocytes, approximately 80% of which were PMN. A strain of E. coli labelled by 32P served as test organism.
D001431:Bacteriological Techniques; D003114:Colony-Forming Units Assay; D003470:Culture Media; D004926:Escherichia coli; D006801:Humans; D009504:Neutrophils / Q000276:immunology / Q000502:physiology*; D010587:Phagocytosis; D010761:Phosphorus Radioisotopes
D003470:Culture Media; D010761:Phosphorus Radioisotopes