[The influence of pretreatment with specific antibodies on Renal Xenograft Rejection (author's transl)].
26 pig kidneys were perfused in a "Gambro" kidney perfusion machine. The perfusates used were unmodified canine plasma and serum, as well as complement-free plasma, gamma-globulin solutions obtained from dogs immunized against pig leucocytes (ALG) and 2-mercapto-ethanol treated ALG (2 ME-ALG). The typical pig-to-dog xenograft rejection was reproduced by ex vivo perfusion with native dog plasma and serum. Using complement-free dog plasma, ALG and 2 ME-ALG pig kidneys could be perfused for several hours without significant increase in perfusion resistance. 22 pig kidneys, pretreated by perfusion for 2 hours with complement-free dog plasma, ALG and 2 ME-ALG, were transplanted on to the vessels of the neck of recipient dogs. Grafts pretreated with decomplemented dog plasma and 2 ME-ALG were rejected in the usual fashion within 5 to 20 min. Pretreatment with ALG resulted in a prolongation of graft function for 80 to 100 min. It is suggested that ALG exerted a transient blocking effect on the cytotoxic action of recipient IgM resembling passive enhancement; this phenomenon may be limited to sites with low antigen density, corresponding to in vitro findings of passive enhancement on cells with low surface antigen density.
D000818:Animals; D000906:Antibodies; D000961:Antilymphocyte Serum; D003165:Complement System Proteins; D004285:Dogs; D006085:Graft Survival; D007114:Immunization; D016030:Kidney Transplantation; D007962:Leukocytes / Q000276:immunology; D008623:Mercaptoethanol; D010477:Perfusion; D013552:Swine; D014183:Transplantation, Heterologous
D000906:Antibodies; D000961:Antilymphocyte Serum; D008623:Mercaptoethanol; D003165:Complement System Proteins
Beeinflussung der Nierenxenotransplantation durch Vorperfusion mit spezifischen Antikörpern.