title: Evaluation of EMIT methods for the determination of the five major antiepileptic drugs on an automated kinetic analyser.
abstract: We have evaluated the performance of enzyme-multipled immunoassay methods for the five major antiepileptic drugs on an automated system, the Perkin-Elmer Model KA-150 Kinetic Analyzer. The precision in the normal duplicate mode was found to be in the range of 6% to 10% for all five tests over a typical working day. All EMIT methods were compared to gas-liquid chromatographic procedures and, in addition, the phenytoin and phenobarbital assays were compared to a liquid-chromatographic method. The phenytoin assay was also compared to RIA and to a manual spectroscopic method. In general, most of the comparison studies resulted in acceptable correlation, although one gas chromatographic method did not correlate very well with the phenytoin and phenobarbital immunoassays.
mesh_terms: D000927:Anticonvulsants / Q000097:blood*; D001322:Autoanalysis / Q000295:instrumentation; D002849:Chromatography, Gas; D002853:Chromatography, Liquid; D006801:Humans; D007124:Immunoenzyme Techniques / Q000295:instrumentation / Q000379:methods; D007700:Kinetics; D008722:Methods; D011863:Radioimmunoassay; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000927:Anticonvulsants