A comparison of two creatine kinase MB determinations: a chromatographic versus an immunological method.
Determinations of the MB isoenzyme of creatine kinase by ion-exchange chromatography and by an immunological method show good overall correlation in plasma of patients with proven infarctions, r=0.98. In analyzing myocardial tissue samples, unexpected discrepancies are found between these two methods. An as-yet-unidentified creatine kinase, in chromatography behaving as MM and immunologically undistinguishable from MB, is found.
D002852:Chromatography, Ion Exchange; D003402:Creatine Kinase / Q000097:blood*; D004586:Electrophoresis; D006801:Humans; D007158:Immunologic Techniques; D007527:Isoenzymes / Q000097:blood*; D008722:Methods; D009132:Muscles / Q000201:enzymology; D009203:Myocardial Infarction / Q000201:enzymology
D007527:Isoenzymes; D003402:Creatine Kinase