Optimal macroculture method for studying mitogenic stimulation of turkey lymphocytes.
This report describes a method for culturing turkey lymphocytes in disposable, unwashed glass test tubes with Morton closures and for recovering lymphocytes on fiber glass filters with a cell harvester made of common laboratory equipment for assay of mitogenic stimulation. Optimal conditions for culture were established.
D000818:Animals; D002478:Cells, Cultured; D007158:Immunologic Techniques; D008214:Lymphocytes / Q000502:physiology*; D008934:Mitogens; D014422:Turkeys
D008934:Mitogens