Translation of tobacco mosaic virus RNA In Acetabularia cell cytoplasm.
Isolated Acetabularia nuclei were microinjected with Tobacco Mosaic Virus RNA and then implanted into an anucleate posterior fragment of an Acetabularia cell. Injected RNA was translated in the Acetabularia cytoplasm from the first to twelfth day after implantation of the nuclei. The production of specific virus proteins was detected and localized in the Acetabularia cytoplasm by an immunofluorescence precipitation technique.
D000076:Acetabularia / Q000378:metabolism*; D000460:Chlorophyta / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D014176:Protein Biosynthesis; D012367:RNA, Viral / Q000235:genetics*; D014027:Tobacco Mosaic Virus / Q000235:genetics*; D014764:Viral Proteins / Q000096:biosynthesis*
D012367:RNA, Viral; D014764:Viral Proteins