Ion shower milling: its application to cell membrane removal.
A thickness of about 100 angstroms of the cell membrane of an isolated single freeze-dried neuron of the snail can be etched off with an ion shower milling machine. The calcium content of the cell membrane area was more than one-fifth of the whole cell. The calcium content of the cell membrane area increased during pentylenetetrazole-induced bursting activity.
D000818:Animals; D002118:Calcium / Q000032:analysis*; D002458:Cell Fractionation / Q000379:methods*; D002462:Cell Membrane / Q000032:analysis*; D006652:Histological Techniques; D007477:Ions; D009474:Neurons / Q000032:analysis* / Q000187:drug effects / Q000648:ultrastructure; D010433:Pentylenetetrazole / Q000494:pharmacology
D007477:Ions; D002118:Calcium; D010433:Pentylenetetrazole