title: [Partial suppression of the effect of gene re1A in Fusr-mutant Escherichia coli K-12 cells].
abstract: Mutants of Escherichia coli K-12 re1A+-strain CP 78 resistant to fusidic acid (Fusr) were isolated and forms sensitive to high concentration of leucine (500 g/ml) were selected. When shifted down from nutrient broth to minimal medium M9 with supplemented glucose and required amino acids, these leucine-sensitive mutants continued RNA synthesis and demonstrated the prolonged lag-phase in contrast to the parent strain CP 78. Both properties are known to be characteristic of the Rel- strains. At the same time withdrawal of the required amino acids results in cessation of RNA synthesis in Fusr mutants, in the parent Rel+ strain. Thus, leucine-sensitive Fusr mutants show Rel- phenotype only upon amino acid starvation caused by shift down from nutrient broth to minimal medium.
mesh_terms: D000222:Adaptation, Physiological; D001426:Bacterial Proteins / Q000096:biosynthesis; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D005672:Fusidic Acid / Q000494:pharmacology*; D005796:Genes; D007930:Leucine / Q000494:pharmacology*; D009154:Mutation; D012329:RNA, Bacterial / Q000096:biosynthesis
chemical_list: D001426:Bacterial Proteins; D012329:RNA, Bacterial; D005672:Fusidic Acid; D007930:Leucine
vernacular_title: Chastichnaia supressiia deĭstviia gena re1A v kletkakh Fusr-mutantov Escherichia coli K-12.