title: Cell surface markers for distinguishing different types of rat dorsal root ganglion cells in culture.
abstract: The distribution of three cell surface markers on dissociated cultures of the rat dorsal root ganglion has been investigated using indirect immunofluorescence. Tetanus toxin binds to a nondividing class of cells with neuronal morphology. Ran-1 is expressed by Schwann cells. Thy-1 is expressed both by fibroblasts and by all the neurones. These assignments have been confirmed by detection of any two of these markers in a single culture using antisera conjugated to different fluorochromes. Approximately 20% of the nonneuronal cells in these cultures were negative for all three markers.
mesh_terms: D000818:Animals; D000941:Antigens / Q000032:analysis; D002462:Cell Membrane / Q000032:analysis / Q000276:immunology; D005347:Fibroblasts / Q000032:analysis / Q000276:immunology; D005455:Fluorescent Antibody Technique; D005727:Ganglia, Spinal / Q000166:cytology*; D009474:Neurons / Q000032:analysis*; D051381:Rats; D011955:Receptors, Drug; D012583:Schwann Cells / Q000032:analysis* / Q000276:immunology; D013744:Tetanus Toxin / Q000378:metabolism
chemical_list: D000941:Antigens; D011955:Receptors, Drug; D013744:Tetanus Toxin