Quantitation of antral gastrin cell populations in the dog.
A method for enumerating G cells in dog antral mucosa by a fluorescent antibody technique was shown to be valid with respect to specificity and reproducibility. The state of intracellular gastrin storage does not influence G cell identification by this technique. To obtain values representative of the entire antrum, all G cells in long strips of antral mucosa must be counted. Results are best expressed as G cells per centimeter length of mucosa. Even with large mucosal samples there are considerable variations in G cell density within a single antrum. The "average" dog antrum contains about 35 million G cells. We observed as much as 2-fold range in G cell density from one dog to another. Each animal should, therefore, serve as its own control when influences on G cell populations are to be studied experimentally.
D000818:Animals; D002452:Cell Count; D004285:Dogs; D005455:Fluorescent Antibody Technique; D005753:Gastric Mucosa / Q000032:analysis / Q000166:cytology*; D005755:Gastrins / Q000032:analysis / Q000378:metabolism*
D005755:Gastrins