Improvement in the sensitivity of DNA polymerase I-deficient Escherichia coli for detecting mutagens and carcinogens.
The sensitivity of a polA strain to the antibacterial activity of mutagens and carcinogens may be increased by inserting one or both of the following characteristics, a lexA mutation or the R391 bacterial plasmid. The effects of the lexA mutation and the plasmid appear to be additive. The differential sensitivity of a polA lexA (R391) strain could be adapted as a preliminary screening test for mutagens and potential carcinogens.
D002273:Carcinogens / Q000494:pharmacology*; D004256:DNA Polymerase I / Q000235:genetics; D004260:DNA Repair; D004269:DNA, Bacterial / Q000235:genetics; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics; D009153:Mutagens / Q000494:pharmacology*; D009154:Mutation; D011815:R Factors
D002273:Carcinogens; D004269:DNA, Bacterial; D009153:Mutagens; D004256:DNA Polymerase I