Isolation of antilymphocyte antibodies, with a solid water-insoluble not gel-like immunoadsorbent.
An immunoadsorbent, consisting of a copolymer from acrylnitrile and methacrylic acid's beta-amino-ethylester as the insoluble, not gel-like carrier, 1.3-difluor-4.6-dinitrobenzene as the binding substance and isolated sheep spleen lymphocytes, is described. It may be especially useful for the preparation of "pure antibodies" to cells and greater or smaller antigenic particles. The immunoadsorbent can easily be prepared in an one-step method, it is stable, and high flow rates can be obtained in column chromatography.
D000961:Antilymphocyte Serum / Q000032:analysis*; D002449:Cell Aggregation; D003602:Cytotoxicity, Immunologic; D006384:Hemagglutination; D007074:Immunoglobulin G / Q000302:isolation & purification*; D007163:Immunosorbent Techniques; D007962:Leukocytes / Q000276:immunology
D000961:Antilymphocyte Serum; D007074:Immunoglobulin G