title: Localization and in vitro synthesis of prostaglandins in components of rabbit preovulatory graafian follicles.
abstract: Graafian follicles obtained 9 hours after the injection of human chorionic gonadotropin (hCG) into mature rabbits were dissected into a follicular fluid component, a granulosa cell-oocyte component, and a residual wall component, (the latter containing mostly theca tissue with a small and variable amount of adhering granulosa cells). The amounts of PGE and PGF were determined for each component. The follicular fluid contained approximately 4-10 times more PGE and PGF than either the granulosa cell-oocyte component or the residual wall component. The latter two components contained approximately equal amounts of these prostaglandins. The in vitro biosynthesis of PGE and PGF was also studied and it was found that the granulosa cell-oocyte component had about 4 fold the capacity of the residual wall, and that the follicular fluid synthesized no prostaglandins. There was no significant effect of LH on either PGE or PGF synthesis in any of the components.
mesh_terms: D000818:Animals; D001826:Body Fluids / Q000378:metabolism; D002469:Cell Separation; D006063:Chorionic Gonadotropin / Q000494:pharmacology; D005260:Female; D006107:Granulosa Cells / Q000378:metabolism; D006652:Histological Techniques; D006801:Humans; D007986:Luteinizing Hormone / Q000494:pharmacology; D009865:Oocytes / Q000378:metabolism; D006080:Ovarian Follicle / Q000378:metabolism*; D011458:Prostaglandins E / Q000096:biosynthesis*; D011460:Prostaglandins F / Q000096:biosynthesis*; D011817:Rabbits
chemical_list: D006063:Chorionic Gonadotropin; D011458:Prostaglandins E; D011460:Prostaglandins F; D007986:Luteinizing Hormone