A rapid objective method for measuring the yeast opsonisation activity of serum.
A simple, objective semi-quantitative assay for yeast opsonisation by normal polymorphs has been developed. This depends on electronically counting the number of free unphagocytosed yeast particles on a Coulter counter. The method has been compared with the widely used microscope technique and shows excellent correlation. The sera of 112 unselected school-children gave a distribution curve consistent with 2 peaks; 7 gave values less than 2 S.D.s below the mean confirming the high incidence of this immunodeficiency.
D002648:Child; D006358:Hot Temperature; D006801:Humans; D008722:Methods; D009895:Opsonin Proteins / Q000378:metabolism*; D010587:Phagocytosis; D012016:Reference Values; D012441:Saccharomyces cerevisiae / Q000276:immunology*
D009895:Opsonin Proteins