The nature of toxicity of human serum in the bioassay for erythropoietin using mouse foetal liver cells.
Using several immunological techniques, it was possible to demonstrate that toxicity of human serum in the foetal mouse liver cell bioassay for erythropoietin was due to complement-dependent IgM hetero antibodies to mouse foetal liver cells. The titre of the antibodies in 50 normal human sera ranged between 2 and 64 as measured in an agglutination test with mouse erythrocytes. Specificity of the antibodies for the ABO-or Ii-blood group system could not be established. Inactivation of complement by heating a serum for 30 minutes at 56 degrees C abolished toxicity.
D000372:Agglutination Tests; D000818:Animals; D000906:Antibodies / Q000032:analysis; D001681:Biological Assay; D001769:Blood; D003165:Complement System Proteins; D004921:Erythropoietin / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D006418:Heme / Q000096:biosynthesis; D006461:Hemolysis; D006358:Hot Temperature; D006801:Humans; D007128:Immunoglobulin Fragments / Q000032:analysis; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008099:Liver / Q000032:analysis / Q000196:embryology / Q000276:immunology; D051379:Mice
D000906:Antibodies; D007128:Immunoglobulin Fragments; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D004921:Erythropoietin; D006418:Heme; D003165:Complement System Proteins