[Molecular cloning of the structural genes of the Escherichia coli deo-operon in plasmid RSF2124].
A specialized phage lambda ddeo carrying the deo operon of Escherichia coli is analyzed by exposing the DNA to the specific restriction endonucleases EcoRI and BamHI. Using the lambda ddeo DNA fragment, obtained by digestion with BamHI and plasmid RSF2124 as a vehicle, the hybrid plasmid pAM1 carrying all the genes of the deo operon is constructed and cloned in E. coli cells. It is shown that the activity of thymidine phosphorylase in the strain AM061, which contains hybrid plasmid pAM1 is 30-fold greater than that in strains of E. coli with chromosomal localization of the deo operon.
D002999:Clone Cells; D003090:Coliphages / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D005838:Genotype; D009876:Operon; D010430:Pentosyltransferases / Q000235:genetics*; D010957:Plasmids; D013939:Thymidine Phosphorylase / Q000235:genetics*
D010430:Pentosyltransferases; D013939:Thymidine Phosphorylase
Molekuliarnoe klonirovanie strukturnykh genov deo-operona Escherichia coli na plazmide RSF2124.