Identification of the amino acid functional groups responsible for 30-S ribosome recognition of messenger RNA.
About 30 protein-selective chemical reagents have been tested for their ability to inhibit the mRNA binding activity of the 30-S ribosome. A number of reagents were investigated which have been shown by other workers to be capable of modifying free epsilon-amino groups of lysine and all were found to inactivate 30-S ribosomes completely for natural mRNA binding activity. Several reagents selective for histidine, tyrosine, and tryptophan were also found to inactivate. We suggest that the epsilon-amino groups of lysine play an important role in mRNA binding to the 30-S ribosome.
D000588:Amines; D000596:Amino Acids; D001665:Binding Sites; D004926:Escherichia coli; D007202:Indicators and Reagents; D007455:Iodine / Q000494:pharmacology; D008239:Lysine; D008299:Maleic Anhydrides / Q000494:pharmacology; D008970:Molecular Weight; D010666:Phenylpropionates / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*; D013388:Succinimides / Q000494:pharmacology; D013439:Sulfhydryl Reagents / Q000494:pharmacology; D014326:Tropanes / Q000494:pharmacology
D000588:Amines; D000596:Amino Acids; D007202:Indicators and Reagents; D008299:Maleic Anhydrides; D010666:Phenylpropionates; D012333:RNA, Messenger; D013388:Succinimides; D013439:Sulfhydryl Reagents; D014326:Tropanes; D007455:Iodine; D008239:Lysine