Cell migration from clotted plasma droplets in vitro: development of a new method for MIF assay.
A new in vitro method for MIF assay has been developed using cells contained in clotted plasma droplets. It gives results concordant with the capillary tube method for H37 Ra Mycobacteria sensitized mice and PPD sensitized healthy persons. The technique is applicable to any migratory cell population and requires only a small number of cells for performance. It is technically simple, not time consuming and practicable both in patients and experimental animals.
D000818:Animals; D001500:BCG Vaccine; D001777:Blood Coagulation; D002464:Cell Migration Inhibition; D002465:Cell Movement; D006801:Humans; D007158:Immunologic Techniques; D008263:Macrophage Migration-Inhibitory Factors / Q000032:analysis*; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D009161:Mycobacterium / Q000276:immunology; D009163:Mycobacterium bovis / Q000276:immunology; D014373:Tuberculin
D001500:BCG Vaccine; D008263:Macrophage Migration-Inhibitory Factors; D014373:Tuberculin