Induction of delayed reaction to dinitrophenylated lymphocytes in guinea pigs with contact sensitivity to DNCB.
The incidence of dinitrophenylated cells in guinea pig lymphocytes incubated with 0-30 mM concentrations of DNBSO3Na in phosphate-buffered saline was examined by an immunofluorescence method using fluorescence-labelled anti-DNP antibody. Under our experimental conditions, the incidence was roughly proportional to the concentration used. Using DNP-lymphocytes as an antigen for skin testing, a marked delayed reaction was induced in guinea pigs sensitized by painting with DNCB and intradermal injection of Freund's complete adjuvant. The significance of these findings is discussed.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D004136:Dinitrobenzenes / Q000494:pharmacology*; D004137:Dinitrochlorobenzene / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D006968:Hypersensitivity, Delayed; D007114:Immunization; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D009578:Nitrobenzenes / Q000276:immunology* / Q000494:pharmacology*; D012882:Skin Tests
D000906:Antibodies; D004136:Dinitrobenzenes; D004137:Dinitrochlorobenzene; D009578:Nitrobenzenes