title: High-performance liquid chromatographic determination of benzodiazepines in human plasma.
abstract: A simple and sensitive method for determination of benzodiazepines in plasma has been developed using high-performance liquid chromatography in a reverse-phase mode. The method is illustrated by application to plasma samples containing diazepam and N-desmethyldiazepam at concentrations which would be encountered during therapy, with limits of detection of 10 ng/ml and 2 ng/ml for diazepam and N-desmethyldiazepam, respectively.
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003975:Diazepam / Q000031:analogs & derivatives* / Q000097:blood*; D006801:Humans; D003708:Nordazepam / Q000097:blood*
chemical_list: D003708:Nordazepam; D003975:Diazepam