title: [Effect of low temperatures (-196 degrees) and of cryoprotectors on some bacterial species].
abstract: The effect of low temperatures (-196 degrees C) and cryoprotectors (PEO-400 and glycerol) on the survival, morphological and functional properties was studied with Escherichia coli, Serratia marcescens and Staphylococcus aureus 209. When the cells were frozen for a short period of time in liquid nitrogen, the survival and the rate of protein synthesis decreased in the gram-negative bacteria but remained almost the same in Staphylococcus aureus. PEO-400 and glycerol manifested cryoprotecting action on all the bacteria under study at concentrations which did not harm the bacteria.
mesh_terms: D001419:Bacteria / Q000187:drug effects* / Q000254:growth & development; D001426:Bacterial Proteins / Q000096:biosynthesis; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects; D005990:Glycerol / Q000494:pharmacology; D012706:Serratia marcescens / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D003451:Cryoprotective Agents; D005990:Glycerol
vernacular_title: Vliianie nizkikh temperatur (-196 gradusov) i krioprotektorov na nekotorye vidy bakteriĭ.