title: Pyridoxine-requiring mutants of Escherichia coli: glycolaldehyde dehydrogenase is not coded for by the pdxB gene.
abstract: Twenty-seven independent pyridoxineless mutants belonging to genetic linkage group I were assayed for glycolaldehyde dehydrogenase. Some mutants lacked enzyme activity entirely, and others showed activity ranging from very low to wild-type levels. Reversion to pyridoxine independence usually had no effect upon this activity. Transfer of the pyridoxine genes to a common host that had wild-type levels of enzyme activity made the recipient pyridoxineless without affecting the activity. These results negate the idea of an obligatory role for glycolaldehyde dehydrogenase in pyridoxine biosynthesis.
mesh_terms: D000079:Acetaldehyde / Q000031:analogs & derivatives; D000445:Aldehyde Oxidoreductases / Q000096:biosynthesis*; D003090:Coliphages; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005796:Genes; D008040:Genetic Linkage; D009154:Mutation; D011736:Pyridoxine / Q000096:biosynthesis*; D014161:Transduction, Genetic
chemical_list: D000445:Aldehyde Oxidoreductases; D000079:Acetaldehyde; D011736:Pyridoxine