Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase.
The construction and analysis of bacterial plasmids that contain and phenotypically express a mammalian genetic sequence are described. Such plasmids specify a protein that has enzymatic properties, immunological reactivity and molecular size characteristic of the mouse dihydrofolate reductase, and render host cells resistant to the antimetabolic drug trimethoprim.
D000818:Animals; D001483:Base Sequence; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D051379:Mice / Q000235:genetics*; D010641:Phenotype; D010957:Plasmids; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D014295:Trimethoprim / Q000494:pharmacology
D004274:DNA, Recombinant; D014295:Trimethoprim; D013762:Tetrahydrofolate Dehydrogenase