Cloning of the lacI gene into A ColE1 plasmid.
The binding of lac repressor to lac operator was utilized to isolate an EcoRI fragment of lambda h80dlac (i+ as well as iq) that contains the lacI gene and lac promoter-operator regions. Ligation of this fragment into EcoRI cleaved pMB9 yielded chimeric DNA molecules of mol. w.t. 9.6 . 10(-6) and 1.5 . 10(7) daltons. Transformed strains containing the plasmids were analyzed for repressor production in vivo and in vitro. Repressor production in one plasmid strain is 7-fold greater than that in heat-inducible lambda h80dlac(iq)lysogens.
D003090:Coliphages / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D004274:DNA, Recombinant / Q000378:metabolism; D004279:DNA, Viral / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D007763:Lac Operon; D012097:Repressor Proteins / Q000096:biosynthesis*; D014157:Transcription Factors / Q000096:biosynthesis*
D004269:DNA, Bacterial; D004274:DNA, Recombinant; D004279:DNA, Viral; D012097:Repressor Proteins; D014157:Transcription Factors