Experimental orchitis induced in rats by passive transfer of an antiserum to seminiferous tubule basement membrane.
A multifocal damage of the testis was obtained when rats were injected intravenously or under the tunica albuginea of the testis with a rabbit antiseminiferous tubule basement membrane serum. The damage was characterized by foci of perivascular and peritubular infiltrates of mononuclear round cells, infolding, thickening, and rupture of the seminiferous tubular wall and different degrees of injury of the germinal epithelium such as, cell disorganization, cell sloughing, and atrophy. Delamination and thickening of seminiferous tubule basement membrane and vacuolization of the Sertoli cell cytoplasm was often observed by electron microscopy. A linear deposit of rabbit gamma-globulin was detected by immunohistochemical techniques along the basement membranes of the seminiferous tubules and vessels. Testicular damage was not detected in rats injected with normal rabbit serum, used as control. In the kidneys of rats injected intravenously with the immune serum, a deposit of rabbit gamma-globulin was detected along glomerular basement membrane. Focal areas of mononuclear cell infiltrates, hypercellularity of glomeruli and thickening of glomerular capillary walls and Bowman's capsule were also observed.
D000818:Animals; D001485:Basement Membrane / Q000276:immunology / Q000473:pathology; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis; D007668:Kidney / Q000473:pathology; D007678:Kidney Glomerulus / Q000276:immunology; D008297:Male; D009920:Orchitis / Q000209:etiology* / Q000473:pathology; D051381:Rats; D012671:Seminiferous Tubules / Q000276:immunology* / Q000473:pathology; D013737:Testis / Q000276:immunology* / Q000648:ultrastructure
D007106:Immune Sera; D007074:Immunoglobulin G