Investigations on the outer membrane proteins of Salmonella typhimurium.
The number, nature and organization of the outer membrane proteins of Salmonella typhimurium have not yet been resolved. Therefore these proteins were isolated using a concentrated solution of guanidine hydrochloride and studied using different analytical techniques. Upon chromatography on Sephadex G-200 four fractions were obtained. Only the fraction containing a protein of molecular weight 13,000 produced immunoprecipitation reactions with the antisera raised against the whole bacteria. On polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate, 7 major proteins were found, with molecular weights between 13,000 and 43,000. Isoelectric focusing on 4.6% polyacrylamide gels resolved the outer membrane proteins into 10 bands with apparent isoelectric points between 5.0 and 8.4. Finally these proteins could be further resolved into as many as 50 spots where a two-dimensional electrophoresis was carried out with isoelectric focusing in the first dimension, and polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate in the second dimension. These results demonstrated that the outer membrane proteins of S. typhimurium are extremely heterogeneous. To investigate the mode of organization of lipopolysaccharides in the outer membrane, the membrane proteins were separated by the liquid isoelectric focusing technique. Lipopolysaccharides were primarily found to be associated with a protein of isoelectric point 7.8.
D001426:Bacterial Proteins / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification; D002473:Cell Wall / Q000648:ultrastructure; D008070:Lipopolysaccharides / Q000032:analysis; D008970:Molecular Weight; D011135:Polysaccharides, Bacterial / Q000032:analysis; D012486:Salmonella typhimurium / Q000276:immunology / Q000648:ultrastructure*
D001426:Bacterial Proteins; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial