Effect of mutations affecting lysyl-tRNAlys on the regulation of lysine biosynthesis in Escherichia coli.
When studying mutants affecting lysyl-tRNA synthetase or tRNAlys (hisT, hisW), a lack of correlation is clearly observed between the amount of lysyl-tRNA and the level of derepression of several lysine biosynthetic enzymes. This exlcudes the possible role of lysyl-tRNA as the specific corepressor of the lysine regulon. However, the level of derepression of DAP-decarboxylase, the last enzyme of the lysine pathway, is very low in the hisT mutant; this indicates that tRNAlys is a secondary effector involved in the regulation of the synthesis of this enzyme.
D000445:Aldehyde Oxidoreductases / Q000235:genetics / Q000378:metabolism; D000604:Amino Acyl-tRNA Synthetases / Q000235:genetics*; D001222:Aspartate Kinase / Q000235:genetics / Q000378:metabolism; D002262:Carboxy-Lyases / Q000235:genetics / Q000378:metabolism; D004794:Enzyme Repression; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D008239:Lysine / Q000096:biosynthesis*; D009154:Mutation; D012343:RNA, Transfer / Q000235:genetics*
D012343:RNA, Transfer; D000445:Aldehyde Oxidoreductases; D001222:Aspartate Kinase; D002262:Carboxy-Lyases; D000604:Amino Acyl-tRNA Synthetases; D008239:Lysine