Core region in Proteus mirabilis lipopolysaccharide.
Four R mutants of P. mirabilis were isolated. The composition of their degraded polysaccharides (PS) obtained from the respective lipopolysaccharides (LPS) as well as the composition and properties of the PS-fractions separated by column chromatography were examined. The results were compared with those obtained with PS of the wild type. One of the mutants could be classified as an Ra-type mutant, presenting a complete LPS core. This polysaccharide core contains: galacturonic acid, glucosamine, glucose, D-glycero-D-mannoheptose, L-glycero-D-mannoheptose in a molar ratio of 1 : 1 : 1 : 1 : 2 and 2-keto-3-deoxyoctonate. Taking into consideration the common sugars described previously in the LPS chemotypes of P. hauseri, the composition of the complete core region mentioned above represents the LPS core part of all the chemotypes, containing two different heptoses.
D001434:Bacteriophage Typing; D002850:Chromatography, Gel; D005944:Glucosamine / Q000302:isolation & purification; D005947:Glucose / Q000302:isolation & purification; D006539:Heptoses / Q000302:isolation & purification; D008070:Lipopolysaccharides; D009154:Mutation; D011135:Polysaccharides, Bacterial / Q000145:classification; D011513:Proteus mirabilis / Q000032:analysis* / Q000235:genetics; D012703:Serotyping; D014574:Uronic Acids / Q000302:isolation & purification
D006539:Heptoses; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D014574:Uronic Acids; D005947:Glucose; D005944:Glucosamine