Action of heparin on protein fractions of isolated nuclei and on their DNA content.
Isolated nuclei of rat hepatocytes were incubated with 0.05% sodium heparinate for 2 to 10 min. Alterations in the nuclei were controlled biochemically, determining the amounts of DNA and histones, and by cytophotometric methods determining the amounts of total and nonhistone proteins and DNA. Under the selected experimental conditions 95% of histones are bound already after 5-min incubation with heparin; nonhistone proteins of cell nuclei remain unchanged. The blockade of histones is followed by DNA diffusion into the incubation medium. Experiments with nuclear staining with alcian blue proved the specificity of heparin binding with histones and showed that heparin-histone complex remains in the nuclei, and its histones lose their extractability with 0.25 n HCl.
D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects*; D003584:Cytological Techniques; D004247:DNA / Q000378:metabolism*; D006493:Heparin / Q000378:metabolism / Q000494:pharmacology*; D006657:Histones / Q000378:metabolism; D008099:Liver / Q000648:ultrastructure*; D008297:Male; D009698:Nucleoproteins / Q000378:metabolism*; D010783:Photometry; D011485:Protein Binding; D051381:Rats; D012995:Solubility; D013347:Subcellular Fractions
D006657:Histones; D009698:Nucleoproteins; D006493:Heparin; D004247:DNA