[Effect of stromal fibroblasts on antibody formation in cultures deficient in A-cells].
Stromal fibroblasts from the monolayer cultures of the rabbit thymus, spleen, and bone marrow when added to suspension cultures of non-adherent rabbit spleen cells had a significant effect on the response of the plaque-forming cells (PFC). Thymus-derived stromal fibroblasts caused an increase in the number of PFC, and bone marrow stromal fibroblasts suppressed the antibody formation in these cultures. Spleen-derived stromal fibroblasts influenced the PFC response similarly to adherent cells. Thymus-derived stromal fibroblasts irradiated by 5000 R influenced the PFC response in the same way as non-irradiated cells. The action of stromal fibroblasts on the antibody formation in the cultures depended on their attachment to the culture flask surface.
D000818:Animals; D000917:Antibody Formation / Q000528:radiation effects; D000921:Antibody-Producing Cells / Q000276:immunology*; D001853:Bone Marrow / Q000276:immunology; D002448:Cell Adhesion; D002460:Cell Line; D005347:Fibroblasts / Q000276:immunology* / Q000528:radiation effects; D006462:Hemolytic Plaque Technique; D009928:Organ Specificity; D011817:Rabbits; D013154:Spleen / Q000276:immunology; D013950:Thymus Gland / Q000276:immunology
Vliianie stromal'nykh fibroblastov na antiteloobrazovanie v kul'turakh defitsitnykh po A-kletkam.