Amplification of the lactose carrier protein in Escherichia coli using a plasmid vector.
The isolation and properties of a hybrid plasmid carrying the Y gene of the lac operon of Escherichia coli are described. The lactose carrier protein, coded for by the Y gene, is readily identified upon lac operon induction in strains carrying the plasmid. The protein comprises about 15% of the cytoplasmic membrane protein synthesized in the first generation after induction, compared with a wild type strain induced under the same conditions where lactose carrier protein comprises 1.4% of the cytoplasmic membrane protein.
D002352:Carrier Proteins / Q000096:biosynthesis*; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics; D007785:Lactose / Q000378:metabolism*; D007786:Lactose Factors; D008565:Membrane Proteins / Q000096:biosynthesis; D010957:Plasmids
D002352:Carrier Proteins; D004274:DNA, Recombinant; D008565:Membrane Proteins; D007785:Lactose