title: A mutation affecting the sigma subunit of RNA polymerase changes transcriptional specificity.
abstract: The RNA polymerase mutation, alt-1, affects the sigma subunit and alters the in vitro selectivity of RNA polymerase to parallel the in vivo phenotype. We propose that the mutation changes the distribution of functionally distinct polymerase isomers.
mesh_terms: D002874:Chromosome Mapping; D012321:DNA-Directed RNA Polymerases / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005809:Genes, Regulator; D008040:Genetic Linkage; D009154:Mutation; D009876:Operon; D012808:Sigma Factor / Q000235:genetics*; D013379:Substrate Specificity; D014157:Transcription Factors / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: D012808:Sigma Factor; D014157:Transcription Factors; D012321:DNA-Directed RNA Polymerases