A simple method for cloning and replica plating of mammalian cells using multicellular spheroids.
V 79/4 Chinese hamster cells or HeLa cells grow in Eagle's MEM supplemented with 25 microgram/ml dextran sulphate to form clonal multicellular spheroids. These cell clones, consisting of 5-10(2) cells, are easy to separate, to transfer from one culture vessel into another and grow as normal monolayer colonies on Dederon cloth circles after subculture in Eagle's MEM without dextran sulphate. A simple replica technique is described by which 500 clones can be transfered onto at least 3 replica cloth circles, 10 cm in diameter, with a replica plating efficiency of approximately 100%.
D000818:Animals; D002999:Clone Cells / Q000166:cytology*; D006224:Cricetinae; D003412:Cricetulus; D003470:Culture Media; D003584:Cytological Techniques; D005347:Fibroblasts / Q000166:cytology; D006367:HeLa Cells / Q000166:cytology
D003470:Culture Media