Accumulation of peptidyl tRNA is lethal to Escherichia coli.
A mutant strain of Escherichia coli with temperature-sensitive peptidyl-tRNA hydrolase grows at 30 degrees C but, when shifted to 40 degrees C, dies at rates affected by physiological, pharmacological, and genetical perturbations. The rate of killing correlates with the relative accumulation of peptidyl-tRNA, suggesting that it is responsible for the death of the cells.
D002265:Carboxylic Ester Hydrolases / Q000378:metabolism; D004926:Escherichia coli / Q000254:growth & development* / Q000378:metabolism; D009154:Mutation; D010455:Peptides; D012329:RNA, Bacterial / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism*; D012346:RNA, Transfer, Amino Acyl; D013696:Temperature
D010455:Peptides; D012329:RNA, Bacterial; D012346:RNA, Transfer, Amino Acyl; C019037:tRNA, peptidyl-; D012343:RNA, Transfer; D002265:Carboxylic Ester Hydrolases; C019674:aminoacyl-tRNA hydrolase