The measurement of total serum proteins by the Biuret method.
The biuret reaction for proteins provides a simple and precise method for measuring serum proteins; Beer's law is obeyed to at least 10 g per dl. Several stable biuret reagents are available. Hemoglobin is the only important cause of interference which cannot be minimized by use of a sample blank. The mechanism of the biuret reaction is described and attention is drawn to the heterogeneity of the serum proteins and to the use of a certified albumin standard.
D001738:Biuret Reaction / Q000266:history*; D001798:Blood Proteins / Q000032:analysis; D056246:Chemistry, Analytic / Q000266:history*; D049672:History, 19th Century; D049673:History, 20th Century
D001798:Blood Proteins