On the mechanisms of induced somatic recombination by certain fungicides in Aspergillus nidulans.
Four fungicides interfered with the segregation of chromosomes at mitosis of Aspergillus nidulans by increasing the somatic recombination, shown as colour sectors in green colonies, in a strain heterozygous for spore colour mutations. In an attempt to discover the mechanisms by which these fungicides increased the somatic recombination, a prototrophic diploid strain, heterozygous for colour and several other appropriate markers in all chromosomes, was used which enabled the detection and classification of all colour recombinants to be made by genetic analysis. The fungicides investigated were: benomyl (methyl-1-(butylcarbamoyl)-2-benzimidazole carbamate) a benzimidazole derivative, botran (2,6-dichloro-4-nitroaniline) and chloroneb (1,4-dichloro-2,5-dimethoxybenzene) of the aromatic hydrocarbon group of fungicides, and the antibiotic actinomycin D. At least three different mechanisms, non-disjunction, mitotic crossing-over and breakage-deletion, were found to be responsible for the recombinogenic activity of the compounds studied.
D000814:Aniline Compounds / Q000494:pharmacology*; D001233:Aspergillus nidulans / Q000235:genetics*; D001542:Benomyl / Q000494:pharmacology*; D002219:Carbamates / Q000494:pharmacology*; D002722:Chlorobenzenes / Q000494:pharmacology*; D002875:Chromosomes / Q000187:drug effects; D003609:Dactinomycin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D011995:Recombination, Genetic / Q000187:drug effects*
D000814:Aniline Compounds; D002219:Carbamates; D002722:Chlorobenzenes; D003609:Dactinomycin; C007009:chloroneb; C004354:dicloran; D001542:Benomyl