A procedure for isolation of spontaneous mutants with temperature sensitive of RNA and/or protein.
A procedure for the isolation of spontaneous temperature sensitive mutants of Escherichia coli has been developed. They are selected as survivors at high temperature against the combined killing effects exerted by a temperature inducible lambda prophage and either streptomycin plus ampillicin or ampicillin plus cycloserine. The mutants so obtained are blocked in vivo in the synthesis of RNA or protein or both at restrictive temperature.
D001426:Bacterial Proteins / Q000096:biosynthesis*; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification; D005821:Genetic Techniques; D006358:Hot Temperature; D009154:Mutation; D012329:RNA, Bacterial / Q000096:biosynthesis*
D001426:Bacterial Proteins; D012329:RNA, Bacterial