The need for a mammalian test system for mutagens: action of some reducing agents.
Reducing agents and cysteine, cysteamine, glutathione, ascorbic acid and H2O2 with and without the addition of Cu2+ did not increase significantly the frequency of mutations in the Salmonella test at non-toxic concentrations but triggered a marked DNA repair synthesis and induced a relatively high frequency of chromosome aberrations in cultured mammalian cells. Both latter effects were reduced by the addition of catalase to solutions of the reducing agents plus Cu2+. To avoid 'False Negatives' in mutagenicity screening the use of several test subjects including mammalian cells seems to be required.
D000818:Animals; D001205:Ascorbic Acid / Q000494:pharmacology; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D003543:Cysteamine / Q000494:pharmacology; D003545:Cysteine / Q000494:pharmacology; D004260:DNA Repair / Q000187:drug effects*; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D005188:False Negative Reactions; D005978:Glutathione / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008322:Mammals; D009153:Mutagens; D012486:Salmonella typhimurium / Q000187:drug effects; D013438:Sulfhydryl Compounds / Q000494:pharmacology
D009153:Mutagens; D013438:Sulfhydryl Compounds; D003543:Cysteamine; D006861:Hydrogen Peroxide; D005978:Glutathione; D003545:Cysteine; D001205:Ascorbic Acid