Escherichia coli heat-labile enterotoxin: comparison of antitoxin assays and serum antitoxin levels.
The mouse adrenal tumor cells (Y-1 strain) and the Chinese hamster ovary cells, two routinely used tissue culture assays for Escherichia coli heat-labile enterotoxin (LT), were used to detect serum antitoxin responses in culture-positive patients from several well-defined sources. There was no correlation between a significant antitoxin response and isolation of LT-producing E. coli in two "domestic" diarrheal outbreaks. Serum samples from a third group of individuals in a rural cholera-endemic area consistently demonstrated significant rises in neutralizing antibody to LT.
D000907:Antibodies, Bacterial / Q000032:analysis*; D000992:Antitoxins / Q000032:analysis*; D001427:Bacterial Toxins; D002460:Cell Line; D003967:Diarrhea / Q000209:etiology / Q000276:immunology*; D004768:Enterotoxins; D004926:Escherichia coli / Q000276:immunology*; D004927:Escherichia coli Infections; D006801:Humans
D000907:Antibodies, Bacterial; D000992:Antitoxins; D001427:Bacterial Toxins; D004768:Enterotoxins