In vitro effects of methyprednisolone sodium succinate and E coli organisms on neutrophils in baboon blood.
The corticosteroid methylprednisolone sodium succinate (MP) has been observed to prevent hypoglycemia in experimental septic shock; however, detrimental actions of various corticosteroids on polymorphonuclear leukocyte function have been reported. The present study was designed to determine if MP depresses glucose metabolism of leukocytes or adversely affects neutrophil survival, or whether it modifies the mortality rate of live E coli in baboon blood in vitro. Results show that therapeutically effective concentrations (13 micrograms/ml blood) and high doses (130 micrograms/ml blood) of MP exert no detrimental influences on glucose utilization or survival of neutrophils in the absence or presence of E coli organisms in concentrations of 4.2 x 10(7) and 2.3 x 10(8) organisms per milliliter blood. However, E coli organisms increase neutrophil mortality rate and glucose uptake of the blood. These findings support the view that MP does not adversely influence leukocyte metabolism and survival, nor does it modify the mortality rate of live E coli.
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D005260:Female; D066298:In Vitro Techniques; D008297:Male; D008775:Methylprednisolone / Q000494:pharmacology*; D009504:Neutrophils / Q000187:drug effects*; D010215:Papio
D001786:Blood Glucose; D008775:Methylprednisolone