DNA sequence for a low-level promoter of the lac repressor gene and an 'up' promoter mutation.
The promoter for a weakly expressed constitutive gene, the lactose repressor gene (lacI), has been sequenced, along with an 'up' promoter mutation Iq. The 10-fold enhancement in I expression found in Iq is the result of a single base change at position -35. To facilitate the sequencing, the lacI gene was cloned in a small plasmid.
D001483:Base Sequence; D001665:Binding Sites; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D005809:Genes, Regulator; D007785:Lactose / Q000235:genetics*; D009876:Operon
D012321:DNA-Directed RNA Polymerases; D007785:Lactose