Quantitative phagocytosis by human polymorphonuclear leucocytes. Use of radiolabelled emulsions to measure thae rate of phagocytosis.
A new micro-method for the quantitative measurement of phagocytosis by neutrophils is described. The material used for phagocytosis consists of a radioactive oil emulsion coated with E. coli lipopolysaccharide. Uptake of radioactive material is a function of cell number, duration of incubation, dilution of serum used for opsonization, content of lipopolysaccharide and concentration of emulsion. This method can be used to quantify rapidly and precisely phagocytosis rates of as few as 5 x 10(4)-10(6) polymorphonuclear leucocytes and the opsonic activity of 10 microliter serum.
D000418:Albumins / Q000494:pharmacology; D002452:Cell Count; D004655:Emulsions; D006801:Humans; D007158:Immunologic Techniques; D007457:Iodine Radioisotopes; D009504:Neutrophils / Q000166:cytology / Q000276:immunology; D009895:Opsonin Proteins / Q000032:analysis; D010587:Phagocytosis
D000418:Albumins; D004655:Emulsions; D007457:Iodine Radioisotopes; D009895:Opsonin Proteins