Mapping of functions in the R-plasmid R388 by examination of deletion mutants generated in vitro.
Mutant plasmids in which large segments of R388 DNA are deleted were constructed in vitro from two R388::TnA (Tn801) plasmids, using the BamHI site of TnA and the BamHI and BglII sites of R388. These deletion mutants permitted mapping of genetic functions into the restriction map of R388.
D004269:DNA, Bacterial / Q000235:genetics*; D004351:Drug Resistance; D004926:Escherichia coli; D005796:Genes; D008040:Genetic Linkage; D010641:Phenotype; D011815:R Factors; D011995:Recombination, Genetic; D013449:Sulfonamides / Q000494:pharmacology; D014295:Trimethoprim / Q000494:pharmacology
D004269:DNA, Bacterial; D013449:Sulfonamides; D014295:Trimethoprim