title: A simplified method for the in vitro induction of IgG antibody in collagen coated dishes.
abstract: Culture dishes precoated with thin layers of acid soluble rat tail collagen simplify conditions necessary to obtain in vitro high IgG anti-DNP responses from primed and boosted mice. In contrast to Mishell-Dutton type cultures the use of collagen precludes the need to continually rock cultures and to add nutrients. Reduction of the cell density in collagen dishes to 10(6) cells/ml is without detriment to the antibody response. Cells adhering to collagen coated dishes after 24 h incubation provide an enriched population of IgG anti-DNP precursor cells; which will mature to antibody secretion at very low cell density (10(4) -10(5) cells/ml). However, as changes in the adhesive properties of cells to collagen occur during cell maturation, attempts to adapt the technique to obtain clones of antibody secreting cells were not wholly successful.
mesh_terms: D000818:Animals; D000921:Antibody-Producing Cells; D002448:Cell Adhesion; D002452:Cell Count; D002478:Cells, Cultured; D003094:Collagen; D003584:Cytological Techniques; D007074:Immunoglobulin G / Q000096:biosynthesis*; D051379:Mice; D013154:Spleen / Q000276:immunology
chemical_list: D007074:Immunoglobulin G; D003094:Collagen