In vitro effect of LH-RH on RNA content and synthesis in isolated anterior pituitary cells from intact and castrated female rats.
The in vitro effect of LH-RH on isolated anterior pituitary cells from intact and castrated female rats has been examined. The cells isolated by trypsin maintained their viability and the ability to response to LH-RH by the release of LH. During the incubation period LH-RH significantly reduced the uridine incorporation into RNA in anterior pituitary cells from both intact and castrated females. There was a time- and dose-dependent response correlation. The RNA content was not affected by LH-RH after 2 h incubation. The administration of the LH-RH with estradiol showed a similar effect.
D000818:Animals; D002369:Castration; D004247:DNA / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology; D005260:Female; D005472:Fluorouracil / Q000494:pharmacology; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology*; D066298:In Vitro Techniques; D007986:Luteinizing Hormone / Q000378:metabolism; D010903:Pituitary Gland, Anterior / Q000187:drug effects / Q000378:metabolism*; D012313:RNA / Q000378:metabolism*; D051381:Rats; D014529:Uridine / Q000378:metabolism
D007987:Gonadotropin-Releasing Hormone; D004958:Estradiol; D012313:RNA; D007986:Luteinizing Hormone; D004247:DNA; D005472:Fluorouracil; D014529:Uridine