Immunofluorescent localization of ovine placental lactogen.
The localization of ovine placental lactogen (OPL) was studied using the immunofluorescence method. The placentome sections were treated by an indirect technique with anti-OPL antibodies obtained from rabbits injected with purified hormone. OPL was located in large cells of the monostratified epithelium of chorionic villi. These cells are mono- or binucleated and PAS-positive. The immunological reaction was inhibited by the specific antigen (OPL, 400 microgram/ml undiluted anti-OPL antiserum) but not by ovine prolactin, bovine growth hormone, human placental lactogen nor by any other polypeptidic hormone tested. Reciprocally, the localization of OPL-secreting cells was unsuccessful with antibodies raised against these control hormones.
D000818:Animals; D002824:Chorionic Villi / Q000032:analysis; D004848:Epithelium / Q000032:analysis; D005260:Female; D005455:Fluorescent Antibody Technique; D010920:Placenta / Q000032:analysis*; D010928:Placental Lactogen / Q000302:isolation & purification*; D011247:Pregnancy; D012756:Sheep
D010928:Placental Lactogen