The influence of different fixatives and osmolality on the ultrastructure of the cochlear neuroepithelium.
To investigate which fixative is most suitable for the preservation of cochlear hair cells three main groups of fixatives were used: 1. 3% glutar aldehyde in various buffers (osmolality altered). 2. Glutar aldehyde/paraform aldehyde combination (ad modum Karnovsky). 3. Osmic acid solutions (OsO4) in veronal acetate buffer according to Rhodin (1954). The 2% osmic acid fixative (350--370mosm/kg) resulted in an excellent ultrastructural preservation; an acceptable result was obtained with a 3% glutar aldehyde fixative in 0.1 M sodium phosphate buffer, 600--700 mosm/kg and the Karnovsky's fixative.
D000818:Animals; D002021:Buffers; D003051:Cochlea / Q000648:ultrastructure*; D006168:Guinea Pigs; D006198:Hair Cells, Auditory / Q000648:ultrastructure*; D006652:Histological Techniques; D008465:Mechanoreceptors / Q000648:ultrastructure*; D008928:Mitochondria / Q000648:ultrastructure; D009994:Osmolar Concentration
D002021:Buffers