[Multiplicity of monoamine oxidase: inhibition of mitochondrial monoamine oxidase activity by isopropylhydrazide of D,L-serine].
Isopropylhydrazide of D,L-serine (IHS) inhibits by 50% (at 37 degrees for 10 min) deamination of serotonin or beta-phenylethylamine by monoamine oxidases from bovine brain stem mitochondrial membranes at the 2.6 X X 10(-5) M or 9 X 10(-5) M, respectively. In order to inhibit by 50% the deamination of tyramine under the same conditions a considerably lower (2.5 X X 10(-6) M) concentration of IHS is required. Kinetic studies of inhibition of enzymatic deamination of all the three biogenic monoamines by IHS showed that the irreversible blocking of the monoamine oxidase activity is preceeded by formation of dissociating enzyme-inhibitor complexes. Values of the dissociation constants of these complexes measured (at 37 degrees) with serotonin, phenylethylamine or tyramine as substrates for estimation of the residual monoamine oxidase activity are 0.47; 0.13 or 0.023 mM, respectively. Significant differences are also found between thermodynamic and activation parameters characterizing both  both steps of interaction between IHS and the monoamine oxidases of mitochondrial membranes in the experiments with serotonin, phenylethylamine or tyramine as substrates. The data obtained suggest the existence of different monoamine oxidases (or their active sites) catalyzing oxidative deamination of serotonin, phenylethylamine or tyramine in the fragments of mitochondrial membranes from bovine brain stem.
D000818:Animals; D001933:Brain Stem / Q000201:enzymology*; D002417:Cattle; D003641:Deamination; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation; D006834:Hydrazines / Q000494:pharmacology; D007163:Immunosorbent Techniques; D007700:Kinetics; D008928:Mitochondria / Q000201:enzymology; D008996:Monoamine Oxidase Inhibitors; D012694:Serine / Q000031:analogs & derivatives*; D013379:Substrate Specificity
D006834:Hydrazines; D008996:Monoamine Oxidase Inhibitors; D012694:Serine
O mnozhestvennosti monoaminoksidaz: tormozhenie izopropilgidrazidom D,L-serina monoamikonsidaznoĭ aktivnosti mitokhondriĭ.