Isolation of DNA from Aspergillus nidulans.
A procedure for isolation of DNA from Aspergillus nidulans on a preparative scale is described. Mechanical disruption of lyophilized material in high-salt medium and treatment with proteinase K, followed by sedimentation of the lysate into saturated CsC1 solution yielded pure, highly polymerized DNA.
D001233:Aspergillus nidulans / Q000032:analysis*; D002499:Centrifugation, Density Gradient; D002846:Chromatography, Affinity; D004247:DNA / Q000302:isolation & purification*; D008722:Methods; D010636:Phenols; D014461:Ultracentrifugation
D010636:Phenols; D004247:DNA