title: The nature of toxicity of human serum in the bioassay for erythropoietin using mouse foetal liver cells.
abstract: Using several immunological techniques, it was possible to demonstrate that toxicity of human serum in the foetal mouse liver cell bioassay for erythropoietin was due to complement-dependent IgM hetero antibodies to mouse foetal liver cells. The titre of the antibodies in 50 normal human sera ranged between 2 and 64 as measured in an agglutination test with mouse erythrocytes. Specificity of the antibodies for the ABO-or Ii-blood group system could not be established. Inactivation of complement by heating a serum for 30 minutes at 56 degrees C abolished toxicity.
mesh_terms: D000372:Agglutination Tests; D000818:Animals; D000906:Antibodies / Q000032:analysis; D001681:Biological Assay; D001769:Blood; D003165:Complement System Proteins; D004921:Erythropoietin / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D006418:Heme / Q000096:biosynthesis; D006461:Hemolysis; D006358:Hot Temperature; D006801:Humans; D007128:Immunoglobulin Fragments / Q000032:analysis; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D008099:Liver / Q000032:analysis / Q000196:embryology / Q000276:immunology; D051379:Mice
chemical_list: D000906:Antibodies; D007128:Immunoglobulin Fragments; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D004921:Erythropoietin; D006418:Heme; D003165:Complement System Proteins