title: [Trials to obtain precipitating antisera against heat-treated soy protein, sodium caseinate and dry milk].
abstract: Developed were two patterns for the production of precipitating sera needed in the demonstration of thermically treated (70 degrees C for 30 minutes) soya-bean proteins, sodium caseinate, and dry milk. The immunization with antigens was carried out at low rates, the immunizing agents being treated after Smidt. According to the first pattern the antigen is first applied subcutaneously with Freund's adjuvant, then subcutaneously on the 15th day, and venously on the 16th and 17th day. According to the second pattern, which is intended for the production of precipitating sera against milk protein the antigen (dry milk) is applied on the 1st, 8th, and 15th day, peritoneally. The test animals are bled on the 26th, resp.21st day. The sera produced are of a high precipitating titer, and show no unspecific reactions with other proteins. They are applied for the demonstration of protein additives in cooked meat products.
mesh_terms: D000818:Animals; D002364:Caseins / Q000276:immunology*; D005503:Food Additives / Q000032:analysis; D007106:Immune Sera / Q000302:isolation & purification*; D007114:Immunization; D007158:Immunologic Techniques; D008892:Milk / Q000276:immunology*; D010940:Plant Proteins / Q000276:immunology*; D011233:Precipitin Tests; D011234:Precipitins / Q000302:isolation & purification*; D011817:Rabbits; D012964:Sodium; D013025:Glycine max; D013696:Temperature; D013997:Time Factors
chemical_list: D002364:Caseins; D005503:Food Additives; D007106:Immune Sera; D010940:Plant Proteins; D011234:Precipitins; D012964:Sodium
vernacular_title: Opiti za dobivane na pretsipitirashti antiserum i sreshtu termichno obraboteni soev beltuk, natriev kazeinat i sukho mliako.