title: Methylation-dependent DNA synthesis in Escherichia coli mediated by DNA polymerase I.
abstract: An in vitro system was used to study DNA synthesis in lysates of Escherichia coli cells which had been grown in the presence of ethionine. Such lysates showed a reduced capacity to incorporate [3H]TTP into high-molecular-weight material. Activity could be restored by incubation with S-adenosyl methionine and ATP. S-adenosyl methionine-reactivated TTP incorporation required the presence of DNA polymerase I, ATP, and all four deoxyribonucleotide triphosphates. DNA polymerase III was not required.
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D002701:Chloramphenicol / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis*; D004256:DNA Polymerase I / Q000378:metabolism*; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D003854:Deoxyribonucleotides / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D005001:Ethionine / Q000494:pharmacology; D008745:Methylation; D012436:S-Adenosylmethionine / Q000378:metabolism*
chemical_list: D003854:Deoxyribonucleotides; D002701:Chloramphenicol; D012436:S-Adenosylmethionine; D000255:Adenosine Triphosphate; D004247:DNA; D004256:DNA Polymerase I; D004259:DNA-Directed DNA Polymerase; D005001:Ethionine