High performance liquid chromatographic analysis of fermentation broths: cephalosporin C and tylosin.
Procedures are described for the analysis of cephalosporin C or tylosin in fermentation broths by high performance liquid chromatography (HPLC). Quantitation of the major components in cephalosporin C broths was done by a system involving separation by an ion-pair technique. Tylosin data were obtained using a reverse phase method. A method of equating uv potency of tylosin to microbiological potency is discussed. A comparison of HPLC with a microbiological method for the determination of tylosin concentration is also made.
D000900:Anti-Bacterial Agents / Q000032:analysis*; D002511:Cephalosporins / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005285:Fermentation; D007933:Leucomycins / Q000032:analysis*; D008828:Microbiological Techniques
D000900:Anti-Bacterial Agents; D002511:Cephalosporins; D007933:Leucomycins