Pairing properties of the methylester of 5-carboxymethyl uridine in the wobble position of yeast tRNA3Arg.
At optimum magnesium concentration (10 mM) both yeast tRNA1Arg and tRNA3Arg are able to bind to poly (A,G) and A-G-A in presence of Escherichia coli robisomes. With A-G-G only tRNA1Arg ginds, wherea tRNA3Arg (anticodon mcm5 U-C-U) is not bound. This result means that the methylcarboxymethyl substituant in position 5 of U prevents its wobble with G.
D000926:Anticodon / Q000378:metabolism*; D001120:Arginine / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites; D003062:Codon / Q000378:metabolism*; D011131:Polyribonucleotides / Q000378:metabolism; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism*; D012343:RNA, Transfer / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D014529:Uridine / Q000031:analogs & derivatives* / Q000378:metabolism
D000926:Anticodon; D003062:Codon; D011131:Polyribonucleotides; D012333:RNA, Messenger; D012343:RNA, Transfer; D001120:Arginine; D014529:Uridine