[Use of an immunoadsorbent in differentiating B and D salmonelloses in poultry].
An immunoadsorbent was obtained from a lipopolysaccharide of Salmonella gallinarum-pullorum, employing the method of Eskenazy. It was used to adsorb the O agglutinating antibodies against S. gallinarum-pullcorum, S. enteritidis, S. heidelberg in blood sera of birds. Adsorption of sera was carried out at room temperature for 40 min. It was demonstrated that the product was highly specific. It fully and specifically found the O agglutinating antibodies in the sera of birds infected with S. gallinarum-pullorum and S. enteritidis and in the sera of birds coming from a flock with pullorum infection, however, did not bind the O agglutinating antibodies induced by the O antigens 4 and 5 characteristic of Salmonella heidelberg, resp., group B. A following investigation of the sera, using a S. heidelberg test-antigen, specified the sera of birds infected with S. heidelberg only. The application of the immunoadsorbent makes it possible to speed up the adsorption of the O agglutinating antibodies, and thus, for one or several hours within a single working day the differentiation is carried out of B and D Salmonella infections in birds.
D000327:Adsorption; D000372:Agglutination Tests; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D000936:Antigen-Antibody Complex; D002645:Chickens; D003937:Diagnosis, Differential; D005260:Female; D007164:Immunosorbents; D008070:Lipopolysaccharides / Q000302:isolation & purification; D011201:Poultry Diseases / Q000175:diagnosis*; D012475:Salmonella / Q000276:immunology; D012481:Salmonella Infections, Animal / Q000175:diagnosis*; D012477:Salmonella enteritidis / Q000276:immunology
D000907:Antibodies, Bacterial; D000936:Antigen-Antibody Complex; D007164:Immunosorbents; D008070:Lipopolysaccharides
Izpolzvane na imunoadsorbent za diferentsirane na B i D salmonelozi po ptitsite.