Compact Escherichia coli nucleoids in a highly supercoiled conformation.
Escherichia coli nucleoids were visualized in a compact, highly supercoiled conformation at 1 M NaCl. After the salt concentration was lowered to 0.15 M NaCl, the nucleoids uncoiled in beaded fibers in which RNA-mediated structural domains could be distinguished.
D002876:Chromosomes, Bacterial / Q000648:ultrastructure*; D004269:DNA, Bacterial; D004278:DNA, Superhelical; D004926:Escherichia coli / Q000648:ultrastructure*; D012260:Ribonucleases / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D014357:Trypsin / Q000494:pharmacology
D004269:DNA, Bacterial; D004278:DNA, Superhelical; D012965:Sodium Chloride; D012260:Ribonucleases; D014357:Trypsin