The use of low ionic strength solution (LISS) in elution experiments and in combination with papain-treated cells for the titration of various antibodies, including eluted antibody.
The investigation presents evidence for the value of suspending red cells in a low ionic strength medium for elution experiments since this has the effect of increasing the uptake of antibodies by the red cells. In addition it has been shown that for the titration of low affinity antibodies, and also of eluted antibodies, the combining capacity of which may be impaired through the elution process, it is an advantage to use a LISS-enzyme (papain) technique.
D000937:Antigen-Antibody Reactions; D003298:Coombs Test; D004912:Erythrocytes / Q000187:drug effects / Q000276:immunology*; D006801:Humans; D007158:Immunologic Techniques; D007477:Ions; D010206:Papain
D007477:Ions; D010206:Papain