[Conversion of nucleoside triphosphates in an RNA polymerase system].
The behavior of nucleoside triphosphates (NTP) in the RNA-polymerase system from E. coli was studied. The conversion of NTP to the corresponding nucleoside diphosphate (NDP) was observed. This phenomenon was accounted for a contaminating enzyme activity in the RNA-polymerase preparations. The possibility to remove such a contamination was demonstrated, the best technique being the DNA-cellulose affinity chromatography. The purified enzyme does not catalyze the intermediate formation of NDP's from NTP's during RNA synthesis with poly(U) and poly(dG)-poly(dC) as templates.
D000255:Adenosine Triphosphate; D002384:Catalysis; D003570:Cytidine Triphosphate; D012321:DNA-Directed RNA Polymerases; D004926:Escherichia coli / Q000201:enzymology* / Q000378:metabolism; D006160:Guanosine Triphosphate; D007700:Kinetics; D009711:Nucleotides; D012329:RNA, Bacterial / Q000096:biosynthesis; D014544:Uridine Triphosphate
D009711:Nucleotides; D012329:RNA, Bacterial; D003570:Cytidine Triphosphate; D006160:Guanosine Triphosphate; D000255:Adenosine Triphosphate; D012321:DNA-Directed RNA Polymerases; D014544:Uridine Triphosphate
O prevrashcheniiakh nukleozidtrifosfatov v RNK-polimeraznoĭ sisteme.