Dextran sulfates as a contaminant of DNA extracted from concentrated viruses and as an inhibitor of DNA polymerases.
Dextran sulfate is commonly used with polyethylene glycol to concentrate viruses before extraction of their DNA. However, dextran slulfate then easily contaminated such DNA and acted as a potent inhibitor of DNA polymerases from Bacillus subtilis (III), phage PBS2, and phage T4. Dextran sulfate only weakly inhibited Micrococcus luteus and Escherichia coli DNA polymerase I preparations.
D001435:Bacteriophages / Q000201:enzymology*; D003090:Coliphages / Q000201:enzymology*; D004279:DNA, Viral; D003911:Dextrans / Q000494:pharmacology*; D004926:Escherichia coli / Q000201:enzymology; D008828:Microbiological Techniques; D008837:Micrococcus / Q000201:enzymology; D019384:Nucleic Acid Synthesis Inhibitors
D004279:DNA, Viral; D003911:Dextrans; D019384:Nucleic Acid Synthesis Inhibitors