title: Mutator activity of a short Okazaki fragment mutant of Escherichia coli.
abstract: A mutant of Escherichia coli (sof) which was previously shown to have increased recombination frequency, to produce abnormally short "Okazaki fragments," and to be deficient in deoxyuridine triphosphatase has now been found also to possess mutator activity for several genes; point mutation rates and deletion rates are affected. The mutational stimulation effects are consistent with the hypothesis that incorporation of uracil into DNA is directly or indirectly responsible for the observed mutator activity.
mesh_terms: D004269:DNA, Bacterial / Q000096:biosynthesis / Q000235:genetics*; D003856:Deoxyuracil Nucleotides; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D005796:Genes; D009154:Mutation; D009268:Nalidixic Acid / Q000494:pharmacology; D011755:Pyrophosphatases / Q000096:biosynthesis; D011995:Recombination, Genetic; D012293:Rifampin / Q000494:pharmacology; D014498:Uracil / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D003856:Deoxyuracil Nucleotides; D009268:Nalidixic Acid; D014498:Uracil; D011755:Pyrophosphatases; D012293:Rifampin