title: Mobilization of the Escherichia coli plasmid ColE1 (colicin E1) and ColE1 vectors used in recombinant DNA experiments.
abstract: The Escherichia coli Co1E1 plasmid, which codes for production of colicin E1, is inherently nontransferable (nonconjugative) by bacterial mating. Co1E1 can be transmitted at mating by a process called mobilization if Co1E1 is coresident with a transfer plasmid. Mobilization is governed in part by a Co1E1 gene called mob. Co1E1 is mob+. Several Co1E1 derivatives employed in recombinant DNA experiments, notably pBR313 and pBR322, are mob-. These cloning vehicles are mobilized at markedly reduced frequency relative to Co1E1. E. coli K12 carrying either pBR313 or pBR322 represents a useful host vector system for recombinant DNA experiments and affords a significant degree of biological containment.
mesh_terms: D003086:Bacteriocin Plasmids; D003227:Conjugation, Genetic; D004269:DNA, Bacterial; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics*; D009154:Mutation; D010957:Plasmids; D012106:Research; D012449:Safety
chemical_list: D004269:DNA, Bacterial; D004274:DNA, Recombinant