Ultrastructural demonstration of T cells in cutaneous tissue sections using specific anti-human T cell antiserum.
The presence of T lymphocytes in the situ in cutaneous infiltrates of contact dermatitis and lichen planus has been demonstrated by an immunoelectronmicroscope technique. A specific anti-human T cell antiserum labelled with peroxidase-conjugated anti-immunoglobulin serum was used to reveal the specific human T lymphocyte antigen (HTLA) which was visualized ultrastructurally. This technique shows that labelling is limited to the surface membrane and allows study of the in situ interrelationship of T cells.
D000954:Antigens, Surface / Q000032:analysis*; D002462:Cell Membrane / Q000276:immunology / Q000648:ultrastructure; D003877:Dermatitis, Contact / Q000276:immunology / Q000473:pathology; D006801:Humans; D007124:Immunoenzyme Techniques; D008010:Lichen Planus / Q000276:immunology / Q000473:pathology; D008854:Microscopy, Electron; D012867:Skin / Q000648:ultrastructure*; D013601:T-Lymphocytes / Q000276:immunology / Q000648:ultrastructure*
D000954:Antigens, Surface