Cadaveric renal preservation with hyperosmolar, intracellular hypothermic washout solution and cold storage.
All 54 kidneys obtained from heart-beating cadavers functioned when preserved by a brief washout using a hypothermic, hyperosmolar, hyperkalemic perfusate, followed by cold storage. The duration of preservation ranged from two hours and 57 minutes to 39 hours and 47 minutes. Two other kidneys retrieved from a nonheart-beating cadaver and preserved by the same technique failed to function because of irreversible acute tubular necrosis. Fifty-six consecutive transplant patients were divided into four groups according to the period of preservation. There was no correlation between graft rejection, frequency of post-transplant dialysis, long term graft function and survival time, when the duration of preservation was less than 24 hours. The advantages of this technique included technical simplicity, low cost, minimal risk of graft infection and easy transportation. The two primary disadvantages were an apparent 24 to 30 hour limit of organ preservation with prompt function and the inability to determine intrarenal perfusion pressure during preservation, thereby missing an important parameter of graft viability.
D000293:Adolescent; D000328:Adult; D002102:Cadaver; D002648:Child; D002675:Child, Preschool; D002908:Chronic Disease; D003080:Cold Temperature; D005260:Female; D005921:Glomerulonephritis / Q000601:surgery; D006084:Graft Rejection; D006801:Humans; D007036:Hypothermia, Induced; D007511:Ischemia; D016030:Kidney Transplantation; D008297:Male; D008875:Middle Aged; D009926:Organ Preservation; D009994:Osmolar Concentration; D013530:Surgical Wound Infection / Q000209:etiology; D013997:Time Factors; D014021:Tissue Preservation; D014184:Transplantation, Homologous