[Chronic cell culture infection with the rabies virus].
Three cell cultures chronically infected with fixed rabies virus, strain MNIIVP-74, have been obtained: HEp-2/2, BHK/13S, and RK-13. In the former two cultures, the infectious virus titers were 2.0 to 5.25 Ig LD50/ml. In RK-13 cells, traces of the infectious virus were found. In the chronically infected HEp-2/2 culture the maximum amount of the antigen-containing cells determined by the fluorescent antibody procedure was 60% and in BHK/13S 80%. Chronically infected cultures had a reduced growth rate and were as sensitive as the controls of superinfection with vesicular stomatitis virus. The virus recovered from chronically infected culture produced a disease in mice at later intervals than the original virus used in the same doses.
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002455:Cell Division; D002478:Cells, Cultured / Q000382:microbiology*; D002908:Chronic Disease; D002999:Clone Cells / Q000382:microbiology; D003370:Coturnix; D005455:Fluorescent Antibody Technique; D051379:Mice; D011818:Rabies / Q000382:microbiology*; D011820:Rabies virus / Q000276:immunology / Q000472:pathogenicity*; D014762:Viral Interference
D000956:Antigens, Viral
Khronicheskaia infektsiia kul'tur kletok virusom beshenstva.