[Comparative study of the biological activity of a toxin isolated from burned skin using physicochemical and immunosorption methods].
Highly specific isolation of a toxin from an extract of burned skin was achieved by means of antibodies immobilized on nonsoluble carrier (immunosorbent). The stability of the pure toxin distinctly decreased on storage. The interrelations were studied between manifestations of the biological activity of the toxin preparations and immunochemical purity of the toxin as well as addition of serum albumin.
D000818:Animals; D002056:Burns / Q000378:metabolism*; D002627:Chemistry, Physical / Q000379:methods; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004355:Drug Stability; D004356:Drug Storage; D007163:Immunosorbent Techniques; D007525:Isoelectric Focusing; D051379:Mice; D051381:Rats; D012709:Serum Albumin / Q000494:pharmacology; D012867:Skin / Q000032:analysis*; D013997:Time Factors; D014118:Toxins, Biological / Q000302:isolation & purification* / Q000633:toxicity
D012709:Serum Albumin; D014118:Toxins, Biological
Sravnitel'noe izuchenie biologicheskoĭ aktivnosti toksina, vydelennogo iz obozhzhennoĭ kozhi fiziko-khimicheskim i immunosorbtsionnym metodami.