Simple method for identification of plasmid-coded proteins.
Proteins encoded by plasmid DNA are specifically labeled in UV-irradiated cells of Escherichia coli carrying recA and uvrA mutations because extensive degradation of the chromosome DNA occurs concurrently with amplification of plasmid DNA.
D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics*; D004261:DNA Replication; D004269:DNA, Bacterial / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D010957:Plasmids; D014466:Ultraviolet Rays
D001426:Bacterial Proteins; D004269:DNA, Bacterial