Localization of inorganic phosphate in the pancreatic B cell and its loss on glucose stimulation.
Perifusion experiments have shown that there is a discharge of inorganic phosphate into the medium when insulin secretion from isolated islets is stimulated by glucose. Histochemical and microprobe examination of resting pancreatic islets in the electron microscope shows a specific accumulation of inorganic phosphate adjacent to the plasmalemma and nucleolus of the B (beta) cells. This phossphate is lost from the cells during secretory stimulation of islets with high concentrations of glucose.
D000818:Animals; D001639:Bicarbonates / Q000494:pharmacology; D004577:Electron Probe Microanalysis; D005947:Glucose / Q000494:pharmacology*; D066298:In Vitro Techniques; D007515:Islets of Langerhans / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008854:Microscopy, Electron; D010477:Perfusion; D010710:Phosphates / Q000378:metabolism*; D051381:Rats
D001639:Bicarbonates; D010710:Phosphates; D005947:Glucose