title: Quantitative forward mutation assay in Salmonella typhimurium using 8-azaguanine resistance as a genetic marker.
abstract: We have developed a quantitative forward mutation assay using Salmonella typhimurium, in which resistance to the purine analog 8-azaguanine is used as a genetic marker. We present the assay protocol, the concentration-dependent toxicity and mutagenicity of five known mutagens (N-methyl-N'-nitro-N-nitrosoguanidine, ICR-191, 9-aminoacridine, dimethylnitrosamine, and benzo[a]pyrene), and reconstruction experiments testing the assay for possible bias. The relative merits of forward versus reverse mutation assays are discussed.
mesh_terms: D001375:Azaguanine / Q000378:metabolism / Q000494:pharmacology*; D001692:Biological Transport; D003600:Cytosol / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004352:Drug Resistance, Microbial; D009153:Mutagens / Q000494:pharmacology; D009154:Mutation; D010430:Pentosyltransferases / Q000378:metabolism; D012486:Salmonella typhimurium / Q000187:drug effects*; D014970:Xanthines
chemical_list: D009153:Mutagens; D014970:Xanthines; D010430:Pentosyltransferases; D001375:Azaguanine