Stepless antibody determination with the stick-ELISA technique. Results expressed as multiple of normal activity (MONA).
A means is presented whereby results of antibody determination with ELISA (enzyme-linked immunospecific assay) can be expressed as the multiple of normal activity (MONA) contained in a normal serum pool, assessed by an internal standard. The procedure combines the advantage of stepless titration, inherent in the method of ELISA, with the opportunity to provide the clinician with an imaginable antibody result. It is discussed that independent of ELISA with the evolution of serology as an aid for the diagnosis of parasitic disease the clinician is faced with an evergrowing variety of normal antibody titers caused among others by different sensitivities of the methods and techniques employed. To preclude these differences result communications as MONA is suggested.
D000906:Antibodies / Q000032:analysis*; D000941:Antigens; D004443:Echinococcosis / Q000276:immunology*; D004446:Echinococcus / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D014122:Toxoplasma / Q000276:immunology; D014123:Toxoplasmosis / Q000276:immunology*
D000906:Antibodies; D000941:Antigens