Covalent cross-linking of ribosomal RNA and proteins by methylene blue-sensitized photooxidation.
Ribosomal proteins are covalently cross-linked to ribosomal RNA by irradiation with visible light in the presence of methylene blue and O2. Proteins S3, S4, S5 and S7 from the 30 S subunit of Escherichia coli ribosomes and L2 and L3 from the 50 S subunit are among the cross-linked proteins. S3 and S5 had not previously been identified as RNA-binding proteins.
D001426:Bacterial Proteins; D004926:Escherichia coli / Q000648:ultrastructure; D008751:Methylene Blue; D010084:Oxidation-Reduction; D010777:Photochemistry; D011485:Protein Binding; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D012269:Ribosomal Proteins; D012270:Ribosomes / Q000648:ultrastructure*
D001426:Bacterial Proteins; D012329:RNA, Bacterial; D012335:RNA, Ribosomal; D012269:Ribosomal Proteins; D008751:Methylene Blue