Binding of deoxyribonuclease I to actin: a new way to visualize microfilament bundles in nonmuscle cells.
Intracellular actin-containing fibers can be visualized by indirect immunofluorescence microscopy when they are stained with antibody directed against DNase I. The location of actin-containing fibers in cells appears to be similar to the staining pattern of antibody to actin. Actin fibers were also visualized by direct fluorescent microscopy with rhodamine-conjugated DNase I.
D000199:Actins / Q000378:metabolism*; D000818:Animals; D002642:Chick Embryo; D003593:Cytoplasm / Q000648:ultrastructure*; D003599:Cytoskeleton / Q000648:ultrastructure*; D003851:Deoxyribonucleases / Q000378:metabolism*; D005347:Fibroblasts / Q000648:ultrastructure*; D005455:Fluorescent Antibody Technique; D011485:Protein Binding
D000199:Actins; D003851:Deoxyribonucleases