title: Identification, purification and immunohistochemical detection of the inhibitor from porcine ovarian follicular fluid to compensatory ovarian hypertrophy in mice.
abstract: An inhibitor to compensatory ovarian hypertrophy in mice was detected in porcine follicular fluid. Compensatory hypertrophy was inhibited by treatment with 0.6 ml whole follicular fluid, 0.2 ml of the non-dialysable fraction, or a fraction of the fluid salted out by ammonium sulphate at a saturation of 14.5--18.5%. The salted-out fraction was separable into two peaks by Sephadex G-200 column chromatography and the second peak, detectable as a single band by polyacrylamide gel disc electrophoresis, contained all the inhibitory activity. Specific fluorescence of an antiserum to the second peak was demonstrated at the granulosa cells.
mesh_terms: D000818:Animals; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005110:Extracellular Space / Q000032:analysis; D005260:Female; D005455:Fluorescent Antibody Technique; D051379:Mice; D006080:Ovarian Follicle / Q000032:analysis*; D010053:Ovary / Q000187:drug effects / Q000502:physiology*; D013552:Swine