Lambda phage promoter used to enhance expression of a plasmid-cloned gene.
A 50-fold (or greater) increase in the production of phage 21 repressor was obtained by construction of a plasmid in which the 21cI (repressor) gene could be transcribed from lambdaPL. The enhancement due to increased 21cI gene copy number and transcription from lambdaPL were at least five-fold and ten-fold, respectively. The plasmid was constructed in vitro by recombination of EcoRI-generated DNA fragments. The use of the DNA fragment containing lambdaPL in obtaining expression of cloned genes is discussed.
D003090:Coliphages / Q000235:genetics*; D004261:DNA Replication; D004269:DNA, Bacterial / Q000235:genetics; D004274:DNA, Recombinant / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics*; D005814:Genes, Viral; D010957:Plasmids; D014158:Transcription, Genetic
D004269:DNA, Bacterial; D004274:DNA, Recombinant; D004279:DNA, Viral