Non-specific effects of passive immunization on implantation in the rabbit.
Passive immunization with goat anti-rabbit uteroglobin antiserum prevents implantation in the rabbit. The dose of antiserum was too low to neutralize all of the uteroglobin present on Day 5 of pregnancy, however, and the effect could not be shown to be specific, because 'control' treatments with goat antiserum to chick avidin or normal goat serum also prevented implantation. Non-specific antisera raised in rabbits had little or no effect on implantation. Partial purification of antibodies from the non-specific goat antisera reversed their effect, while anti-uteroglobin gamma globulin still reduced implantation. Fluorescein-labelled gamma globulin fractions of anti-avidin and anti-uteroglobin both bound to blastocysts, but pure FITC-IgG showed binding only of anti-uteroglobin. Both anti-avidin and anti-uteroglobin IgG prevented implantation. It is concluded that the effect on implantation is not necessarily achieved via a specific antigen.
D000818:Animals; D000906:Antibodies; D010064:Embryo Implantation; D005260:Female; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000502:physiology*; D007112:Immunity, Maternally-Acquired; D011247:Pregnancy; D011817:Rabbits; D014598:Uteroglobin / Q000276:immunology / Q000502:physiology*
D000906:Antibodies; D006023:Glycoproteins; D014598:Uteroglobin