[Intergeneric conjugational crossing of Escherichia coli with Salmonella typhimurium. II. Transfer of a polA1 mutation from Escherichia coli to Salmonella typhimurium and its phenotypic expression in the salmonella genome].
The Escherichia coli structural gene for DNA polymerase I was inserted into Salmonella typhimurium chromosome by conjugal transfer. The genetic analysis of P1-mediated transduction of obtained hybrid showed that polA gene is located in it between metE and rha loci and is cotransduced with metE (about 50%) and rha (12%). The phenotypic properties of polA1 hybrid E. coliXS. typhimurium concerning UV-MMS-NG and gamma-ray sensitivity are similar to the polA1 mutants of E. coli.
D003227:Conjugation, Genetic; D004256:DNA Polymerase I / Q000235:genetics*; D004259:DNA-Directed DNA Polymerase / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005838:Genotype; D006824:Hybridization, Genetic; D009154:Mutation; D010641:Phenotype; D012486:Salmonella typhimurium / Q000235:genetics*; D014161:Transduction, Genetic
D004256:DNA Polymerase I; D004259:DNA-Directed DNA Polymerase
Mezhrodovoe kon"iugatsionnoe skreshchivanie Escherichia coli i Salmonella typhimurium. Soobshchenie II. Perenos polA1-mutatsii ot Escherichia coli k Salmonella typhimurium i ee fenotipicheskoe vyrazhenie v genome sal'monell.