Purification of mouse interferon by sequential affinity chromatography on poly(U)--and antibody--agarose columns.
Mouse interferon has been purified to homogeneity by twostep affinity chromatography. Two polypeptide bands were obtained on sodium dodecyl sulphate--polyacrylamide gel electrophoresis migrating at molecular weights 35,000 and 22,000, both having antiviral activity. The 35,000 but not the 22,000 band, also stained with periodic acid--Schiff. The specific activity was 8 X 10(9) of our laboratory units, corresponding to 2.4 X 10(9) NIH reference units.
D000818:Animals; D002455:Cell Division / Q000187:drug effects; D002846:Chromatography, Affinity / Q000379:methods; D006023:Glycoproteins / Q000302:isolation & purification; D007163:Immunosorbent Techniques; D007372:Interferons / Q000302:isolation & purification* / Q000494:pharmacology; D051379:Mice; D008970:Molecular Weight; D011072:Poly U; D014779:Virus Replication / Q000187:drug effects
D006023:Glycoproteins; D011072:Poly U; D007372:Interferons