An antiserum which recognizes lipopolysaccharide-reactive B cells in the mouse.
Rabbit antisera have been prepared against spleen B cells from C3H/Tif mice, high responders to lipopolysaccharide (LPS). After complete absorption on tissues of C3H/Hej mice (LPS nonresponders), such antisera specifically stain and kill (in the presence of complement) a fraction of the B cells from a number of LPS high responder strains, and fail to recognize any cells in two different LPS nonresponder strains, C3H/HeJ and C57B1/10.Sc.Cr. The ontogeny, organ and strain distribution of this B cell marker parallels LPS reactivity. Frequencies of cells stained by this antiserum correspond to the frequencies of LPS-reactive B cell precursors recently determined. This antigent(s) is expressed by LPS-activated B cell blasts but not by cells which remain small after 48 h in the presence of LPS. Removal of the cells recognized by the antisera leads to depletion of LPS-reactive cells, while enriching for reactivity to another B cell mitogen, e.g. lipoprotein.
D000818:Animals; D000918:Antibody Specificity; D000961:Antilymphocyte Serum / Q000494:pharmacology*; D001402:B-Lymphocytes / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D005455:Fluorescent Antibody Technique; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051379:Mice; D008805:Mice, Inbred A; D008806:Mice, Inbred AKR; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D009928:Organ Specificity; D011947:Receptors, Antigen, B-Cell; D013045:Species Specificity; D013154:Spleen / Q000276:immunology
D000961:Antilymphocyte Serum; D008070:Lipopolysaccharides; D011947:Receptors, Antigen, B-Cell