A new analytical method for determining duration of phases, rate of DNA synthesis and degree of synchronization from flow-cytometric data on synchronized cell populations.
L-cells synchronized by mitotic selection were investigated by flow-cytometry and the fractions of cells in the various cell cycle compartments were determined as a function of time. A new analytical evaluation procedure was developed, by which the mean transit-times of cells through various cell cycle phases can be calculated from these data. Three examples for application of the method are presented: (1) determination of the duration of G1, S, G2 + M and of the whole cell cycle; (2) calculation of the rate of DNA synthesis in several subcompartments of the S-phase; and (3) evaluation of the degree of synchronization at different stages of the cell cycle.
D002453:Cell Cycle; D003584:Cytological Techniques; D004247:DNA / Q000096:biosynthesis; D007399:Interphase; D007700:Kinetics; D007739:L Cells; D008433:Mathematics; D008938:Mitosis; D013050:Spectrometry, Fluorescence
D004247:DNA