Absence of DNA sequences homologous to transposable element Tn5 (Kan) in the chromosome of Escherichia coli K-12.
The DNA hybridization procedure of Southern has been used to search for homology between the transposable kanamycin resistance determinant Tn5 and sequences in the chromosome of Escherichia coli K-12. No homology was detected under conditions in which a segment homologous to 5% or more of the 5,300-base pair Tn5 element would have been seen.
D001483:Base Sequence; D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics*; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000235:genetics* / Q000648:ultrastructure; D005796:Genes; D007612:Kanamycin / Q000494:pharmacology; D009693:Nucleic Acid Hybridization
D004269:DNA, Bacterial; D007612:Kanamycin