The origin of the nitrogen atom in the thiazole ring of thiamine in Escherichia coli.
Methods are described for the isolation and gas chromatographic-mass spectrometric analysis of the 4-methyl-5-beta-hydroxyethyl thiazole moiety of thiamine in microbial cells. Using these methods, it was determined that in Escherichia coli the nitrogen atom in the thiazole ring of thiamine is derived solely from L-tyrosine.
D004926:Escherichia coli / Q000378:metabolism*; D013058:Mass Spectrometry; D009584:Nitrogen / Q000378:metabolism; D013045:Species Specificity; D013379:Substrate Specificity; D013831:Thiamine / Q000096:biosynthesis / Q000378:metabolism*; D013834:Thiamine Pyrophosphatase / Q000378:metabolism; D013844:Thiazoles / Q000378:metabolism; D014443:Tyrosine / Q000378:metabolism
D013844:Thiazoles; D014443:Tyrosine; D013834:Thiamine Pyrophosphatase; D009584:Nitrogen; D013831:Thiamine