[Immobilization of proteins on macroporous glasses involving maleinimide as the anchoring group].
Macroporous glasses with pore sizes from 400-1000 A appropriate for protein binding were produced and characterized by a thermal demixing procedure and alkaline after treatment. To achieve a covalent binding capacity relative to proteins, the gamma-aminopropyl derivative was allowed to react with 4-maleinimido benzoylic chloride to give preparations containing, in addition to maleinimide residues, acid chloride structures for the protein binding. A preparation of 400 A pore size was tested for its protein binding capacity relative to bovine serum albumin and trypsin. Furthermore, the capacity of binding glucoamylase from Endomycopsis bispora in active form was studied.
D000818:Animals; D002417:Cattle; D005898:Glass; D005087:Glucan 1,4-alpha-Glucosidase / Q000494:pharmacology; D008301:Maleimides / Q000494:pharmacology*; D011485:Protein Binding / Q000187:drug effects; D012710:Serum Albumin, Bovine / Q000378:metabolism; D014357:Trypsin / Q000097:blood
D008301:Maleimides; D012710:Serum Albumin, Bovine; D005087:Glucan 1,4-alpha-Glucosidase; D014357:Trypsin
Immobilisierung von Proteinen an makroporösen Gläsern unter Beteiligung von Maleinimid als Ankergruppe.