A simple, three-step procedure for the large scale purification of DNA ligase from a hybrid lambda lysogen constructed in vitro.
A simple three-step procedure for the large scale purification of DNA ligase has been developed. THe source of enzyme is a strain of Escherichia coli with a hybrid lambda prophage constructed in vitro that bears the ligase overproducing gene lop 11 lig+ (Panasenko, S., Cameron, J., Davis, R. W., and Lehman, I. R. (1977) Science 196, 188-189). The procedure yields homogeneous enzyme in approximately 40% yield.
D003090:Coliphages / Q000235:genetics; D011088:DNA Ligases / Q000302:isolation & purification*; D004926:Escherichia coli / Q000201:enzymology; D008242:Lysogeny; D008722:Methods; D011118:Polynucleotide Ligases / Q000302:isolation & purification*
D011088:DNA Ligases; D011118:Polynucleotide Ligases