Differentiation of rennet from other milk-clotting enzymes by polyacrylamide gel electrophoresis.
Polyacrylamide gel electrophoresis was used to differentiate animal rennet and other milk-clotting enzymes. After electrophoresis, the separated components were visualized by staining with aniline blue-black. Two prominent proteins were found in calf and bovine rennet, while only 1 major protein was observed in pepsin and enzymes of microbial origin. These patterns provided a basis for distinguishing animal rennet and the other enzymes as well as a means of identifying each type of enzyme by the characteristic pattern shown.
D000818:Animals; D002417:Cattle; D012085:Chymosin / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D008892:Milk / Q000201:enzymology*
D012085:Chymosin