Genetic analysis of the human cell surface: antigenic marker for the human X chromosome in human-mouse hybrids.
Somatic cell hybrids of human fibroblasts and mouse A9 cells, carrying only a portion of the human X chromosome in a mouse chromosome background, were injected into C3H mice. The resulting mouse anti-hybrid cell antisera contain antibodies found to be human specific and to react with only those hybrid cells carrying the human X chromosome, as confirmed by essentially perfect concordance between antibody binding assayed by indirect immunofluorescence and presence of the human X-linked enzyme hypoxanthine phosphoribosyl-transferase determined by autoradiographic assay of [3H] hypoxanthine utilization. Heterogeneous mixtures of hybrid cells may be analyzed into fluorescent (X plus) and nonfluorescent (X minus) subpopulations and fractionated viably by using a fluorescence-activated cell sorter.
D000818:Animals; D000941:Antigens / Q000032:analysis*; D002462:Cell Membrane / Q000276:immunology; D002469:Cell Separation / Q000379:methods; D002877:Chromosomes, Human; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D006822:Hybrid Cells / Q000276:immunology / Q000648:ultrastructure; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics; D051379:Mice; D012730:Sex Chromosomes; D014960:X Chromosome
D000941:Antigens; D007041:Hypoxanthine Phosphoribosyltransferase