An indirect, quantifiable assay for cytophilic antibody.
An indirect, quantifiable method for in vitro measurement of anti-SRBC antibody cytophilic for macrophages is described. The procedure consists of adding dilutions of normal or immune serum to 4 X 10(6) macrophages in 35 mm plastic petri dishes for 1 h at 4 degrees C. The plates are throughly washed to remove unbound antibody and 2 X 10(7) 51Cr-SRBC are added for 2 h at 4 degrees C. Total cpm in the plates is determined, non-bound 51Cr-SRBC are removed by washing and the plates are re-counted. Percent binding is calculated from these two values. The assay provides data consistent with visual determinations of number of SRBC/100 macrophages but has less variability and is performed more rapidly and easily than the visual method.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D007158:Immunologic Techniques; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D012756:Sheep; D013696:Temperature
D000906:Antibodies