Evidence for synthesis of alkaline phosphatase on membrane-bound polysomes in Escherichia coli.
Polysomes containing nascent chains of alkaline phosphatase have been isolated from a membrane-bound polysome preparation. Indirect immunoprecipitation using conformation-specific antibodies has been employed. This technique provides a good enrichment of these polyribosomes since routinely no more than than 10--15% of non-specific immunoprecipitation was observed. The yield of the procedure is generally 40% but can be increased if higher non-specific immunoprecipitation is tolerated. Antibodies, previously described, directed against uncoiled or folded monomers of alkaline phosphatase can be used as primary antibody to recognize the nascent chains contained in membrane-bound polysomes which suggests that these chains are partially folded.
D000469:Alkaline Phosphatase / Q000096:biosynthesis* / Q000276:immunology; D000906:Antibodies; D004926:Escherichia coli / Q000201:enzymology*; D046911:Macromolecular Substances; D008566:Membranes / Q000201:enzymology*; D011132:Polyribosomes / Q000201:enzymology*
D000906:Antibodies; D046911:Macromolecular Substances; D000469:Alkaline Phosphatase