[Enzyme-linked immunoabsorbent assay of factor VIII-related antigen. Interest in study of Von Willerbrand's disease (author's transl)].
Factor VIII-related antigen is measured by ELISA as follows: Factor VIII-R: Ag is first "captured" between rabbit antibodies to human factor VII R : Ag adsorbed in polystyrene tubes, and conjugate (peroxydase-labelled rabbit antibodies). A chromogenic enzyme substrate is then added and absorbence measurement allows subsequent assay. This method is sensitive, reproducible and specific. The comparison between the results obtained by this enzyme immuno-assay and the electro-immunodiffusion assay (EIA) is interesting for the study of quantitative and qualitative variations of F VII R : Ag in von Willebrand's disease. Two observations of von Willerbrand variants are described in detail : VIII R : Ag plasma level of the first one is normal using EIA but decreased using ELISA, its electrophoretic mobility is increased; in the second case we found typical anomalies of von Willebrand except the VIII R : Ag level normal by the two methods.
D000941:Antigens / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay; D005169:Factor VIII / Q000032:analysis / Q000276:immunology*; D005260:Female; D006801:Humans; D007526:Isoelectric Point; D008297:Male; D014842:von Willebrand Diseases / Q000097:blood* / Q000276:immunology; D014841:von Willebrand Factor / Q000032:analysis
D000941:Antigens; D014841:von Willebrand Factor; D005169:Factor VIII
Dosage de l'antigéne lié au facteur VIII par la technique ELISA. Intérêt dans l'étude de la maladie de Willebrand.