Practical colorimeter for direct measurement of microplates in enzyme immunoassay systems.
A colorimeter capable of measuring results of enzyme-linked immunosorbent assay (ELISA) reactions directly in the wells of a microtiter plate is described. This colorimeter proved to be as accurate as a conventional spectrophotometer in assessing ELISA reactions, but had the advantage of not requiring transfer of the specimen to a separate chamber. With this colorimeter, 96 specimens can be read in approximately 5 min. A practical colorimeter such as this can make the use of ELISA tests more feasible for many laboratories.
D003124:Colorimetry / Q000295:instrumentation*; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation*; D005069:Evaluation Studies as Topic; D005243:Feces / Q000382:microbiology; D006801:Humans; D007124:Immunoenzyme Techniques / Q000295:instrumentation*; D012087:Reoviridae / Q000302:isolation & purification; D013053:Spectrophotometry / Q000295:instrumentation