[Use of 51Cr-labelled macrophages for the objective evaluation of macrophage migration inhibition].
A possibility of using 51Cr-labeld macrophages migrating from the capillaries and lysed with sodium dodecyl sulfate for objective and quantitative assessment of the results of macrophage migration inhibition (MMI) microassay was demonstrated on the basis of correlation analysis of the experimental data. Migration area and sensitivity of 51Cr-labeled macrophages to the action of the factor of migration inhibition were found not to differ from those of unlabeled macrophages. In the assessment of the absolute macrophage migration values and its reduction under the action of the factor of migration inhibition formed in the H-2 system, and also of the specificity of this reduction there was revealed a high correlation between the migration area and the value of the migrant cell label.
D000818:Animals; D002464:Cell Migration Inhibition; D002860:Chromium Radioisotopes; D007114:Immunization; D007158:Immunologic Techniques; D008263:Macrophage Migration-Inhibitory Factors / Q000494:pharmacology; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009368:Neoplasm Transplantation; D012513:Sarcoma, Experimental / Q000276:immunology; D014184:Transplantation, Homologous; D014185:Transplantation, Isogeneic
D002860:Chromium Radioisotopes; D008263:Macrophage Migration-Inhibitory Factors
Ispol'zovanie mechennykh 51Cr makrofagov dlia ob"ektivnoĭ otsenki podavleniia migratsii makrofagov.