Selection of yeast auxotrophs by thymidylate starvation.
A rapid procedure for the recovery of Saccharomyces cerevisiae auxotrophs was developed by exploiting the protection of these mutants from thymineless death when a required metabolite was withheld. The method can be used for thymidine 5'-monophosphate-requiring auxotrophs or wild-type strains blocked in de novo synthesis of thymidylate by folate antagonists.
D000596:Amino Acids / Q000378:metabolism*; D005796:Genes; D006639:Histidine / Q000378:metabolism; D007930:Leucine / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D008715:Methionine / Q000378:metabolism; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D013938:Thymidine Monophosphate / Q000378:metabolism*; D013942:Thymine Nucleotides / Q000378:metabolism*; D014443:Tyrosine / Q000378:metabolism; D014498:Uracil / Q000378:metabolism
D000596:Amino Acids; D013942:Thymine Nucleotides; D013938:Thymidine Monophosphate; D014443:Tyrosine; D006639:Histidine; D014498:Uracil; D008715:Methionine; D007930:Leucine; D008239:Lysine