Stereo immunofluorescence microscopy: I. Three-dimensional arrangement of microfilaments, microtubules and tonofilaments.
An easy manipulation of the commercial fluorescence microscope allows stereo pairs of pictures to be taken, which when examined with a stereo viewer, give a strong three-dimensional impression. The procedure is described in detail. Its use allows the documentation by immunofluorescence microscopy of the three-dimensional display and organization of microfilament bundles, microtubules and tonofilaments in some well characterized tissue culture cell lines.
D000199:Actins / Q000276:immunology / Q000378:metabolism; D002460:Cell Line; D003593:Cytoplasm / Q000648:ultrastructure*; D003599:Cytoskeleton / Q000648:ultrastructure*; D005455:Fluorescent Antibody Technique; D008856:Microscopy, Fluorescence / Q000379:methods; D008870:Microtubules / Q000648:ultrastructure*; D008871:Microvilli / Q000648:ultrastructure; D009218:Myosins / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism
D000199:Actins; D014404:Tubulin; D009218:Myosins