[Effect of violamycin BI on the process of excision repair in Escherichia coli K 12 following UV irradiation].
The influence of violamycin B I on the process of excision repair of DNA-damages after UV-irradiation has been studied by observing the capacity to rejoin single-strand breaks introduced in the DNA at the beginning of the repair process. The number of single-strand breaks remaining unrepaired in the DNA was higher in presence of violamycin B I. Sedimentation analysis of the DNA of unirradiated cells showed in presence of violamycin B I only a small change in the molecular weight. As a possible reason for the lower capacity of the cells to accomplish repair steps following incision in presence of violamycin B I an inhibition of the function of repair enzymes by interaction of the antibiotic with the DNA-template is discussed.
D000617:Aminoglycosides / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004260:DNA Repair / Q000187:drug effects*; D004269:DNA, Bacterial / Q000096:biosynthesis / Q000528:radiation effects; D004926:Escherichia coli / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D008970:Molecular Weight; D014466:Ultraviolet Rays
D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial
Einfluxss von Violamycin BI auf den Ablauf der Excisionsreparatur in Escherichia coli K12 nach UV-Bestrahlung.