Indirect immunofluorescence microscopy of microtubular structures in male germ cells of wildtype and l(3)pl (lethal-polyploid) Drosophila hydei.
Tubulin-containing structures of the male germ cells of Drosophila hydei crossreact in indirect immunofluorescence microscopy with antibody directed against homogeneous porcine brain tubulin. There is no detectable difference in reactivity between germ cells of wildtype flies and the mutant l(3)pl (lethal-polyploid) which is characterized by microtubular abnormalities. However, the technique of indirect immunofluorescence microscopy allows the direct visualization of several abnormalities in the arrangement of the microtubular system of the mutant, particularly in the axonemal complex.
D000818:Animals; D004330:Drosophila / Q000235:genetics / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D005854:Germ Cells / Q000648:ultrastructure*; D008297:Male; D008856:Microscopy, Fluorescence; D008870:Microtubules / Q000648:ultrastructure*; D009154:Mutation