Chemiluminescent detection of bacteria: experimental and theoretical limits.
The limit of sensitivity of the chemiluminescent assay for detection of bacteria by hemeprotein catalysis of luminol oxidation was determined, both experimentally and theoretically, to be no lower than 10(5) to 10(6) viable bacterial per ml.
D001419:Bacteria / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000378:metabolism; D001431:Bacteriological Techniques; D003470:Culture Media; D004926:Escherichia coli / Q000378:metabolism; D006420:Hemeproteins / Q000378:metabolism; D049449:Luminescence; D008165:Luminol / Q000378:metabolism; D010084:Oxidation-Reduction; D011514:Proteus vulgaris / Q000378:metabolism; D013211:Staphylococcus aureus / Q000378:metabolism; D014556:Urine
D001426:Bacterial Proteins; D003470:Culture Media; D006420:Hemeproteins; D008165:Luminol