title: Reconstitution and purification of flagellar filaments from Caulobacter crescentus.
abstract: Filaments from isolated flagella of Caulobacter crescentus have been purified by successive dissociation and reconstitution. After the second and third reconstitutions from subunits in 0.8 M sodium citrate, filament preparations contained only two proteins, flagellin A (26,000 daltons) and flagellin B (28,000 daltons). There was some enrichment for flagellin A during reconstitution by this procedure, since isolated flagella contained flagellin A and flagellin B in a ratio of approximately 3.8:1 and filaments after the third reconstitution contained the two proteins in a ratio of 5.0:1.
mesh_terms: D001419:Bacteria / Q000648:ultrastructure*; D001426:Bacterial Proteins / Q000032:analysis*; D001431:Bacteriological Techniques; D002458:Cell Fractionation; D005407:Flagella / Q000032:analysis*; D005408:Flagellin / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D001426:Bacterial Proteins; D005408:Flagellin