Visualization of central noradrenergic neurons in thick sections by the unlabeled antibody method: a transmitter-specific Golgi image.
The unlabeled peroxidase-antiperoxidase method has been used with an antiserum against rat dopamine-beta-hydroxylase (DBH) to obtain a three-dimensional image of noradrenergic cell bodies and their processes in thick Vibratome sections of rat brain. This method stains DBH-positive neurons exclusively with a result similar to that of the Golgi method, which makes it possible to analyze the geometric plan of these neurons and their projections in the central nervous system. In 100-micron sections, DBH-positive axons can be followed over long distances, and the results indicate that their distribution in cerebral and cerebellar cortex is not diffuse but has a strict geometric order.
D000818:Animals; D001369:Axons / Q000201:enzymology; D001921:Brain / Q000166:cytology* / Q000201:enzymology; D001933:Brain Stem / Q000166:cytology; D002531:Cerebellum / Q000166:cytology / Q000201:enzymology; D002540:Cerebral Cortex / Q000166:cytology / Q000201:enzymology; D003712:Dendrites / Q000201:enzymology; D004299:Dopamine beta-Hydroxylase / Q000276:immunology / Q000378:metabolism*; D007124:Immunoenzyme Techniques; D051381:Rats
D004299:Dopamine beta-Hydroxylase