A simple method for the preparation and storage of helper T cells.
Relatively purified populations of helper T cells free of active PFC precursors can be prepared from primed splenocytes by irradiation, hypotonic shock, and filtration over glass wool. These purified populations can be stored and helper activity recovered undiminished after up to 6 months of cryopreservation.
D000818:Animals; D002469:Cell Separation; D003451:Cryoprotective Agents / Q000494:pharmacology*; D005374:Filtration; D006462:Hemolytic Plaque Technique; D007038:Hypotonic Solutions; D008722:Methods; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D011309:Preservation, Biological; D013601:T-Lymphocytes / Q000276:immunology* / Q000528:radiation effects
D003451:Cryoprotective Agents; D007038:Hypotonic Solutions