The testis as a secretory organ for H-Y antigen.
After cultivation of dissociated rat testicular tissues, H-Y antigen is detectable in the medium; this is not the case if nongonadal male tissues are incubated. Release of H-Y antigen by testis cells is inhibited by the addition of cycloheximide. All tissues still type H-Y positive after culture. It is assumed that the testis actively secretes H-Y antigen. This assumption is supported by the finding that the amount of H-Y antigen in the epididymal fluid increases with the age of the animals.
D000818:Animals; D000954:Antigens, Surface; D004822:Epididymis / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006182:H-Y Antigen; D007668:Kidney / Q000276:immunology; D008099:Liver / Q000276:immunology; D008297:Male; D051381:Rats; D013154:Spleen / Q000276:immunology; D013737:Testis / Q000276:immunology / Q000378:metabolism*
D000954:Antigens, Surface; D006182:H-Y Antigen