title: N10-Formyltetrahydrofolate is the formyl donor for glycinamide ribotide transformylase in Escherichia coli.
abstract: Glycinamide ribotide transformylase from Escherichia coli was obtained free of N5,N10-methenyltetrahydrofolate cyclohydrolase activity by DEAE-cellulose chromatography. In reaction mixtures containing this enzyme preparation in potassium maleate buffer, pH 7.2, no detectable interconversion of N5,N10-methenyltetrahydrofolate occurred. Upon addition of glycinamide ribotide, N-formylglycinamide ribotide was formed when N10-formyltetrahydrofolate was present; no formylation occurred in the presence of N5,N10-methenyltetrahydrofolate. A method for the synthesis and purification of glycinamide ribotide is presented.
mesh_terms: D000217:Acyltransferases / Q000302:isolation & purification*; D004926:Escherichia coli / Q000201:enzymology*; D005561:Formates; D005575:Formyltetrahydrofolates; D005998:Glycine; D007700:Kinetics
chemical_list: D005561:Formates; D005575:Formyltetrahydrofolates; D000217:Acyltransferases; D005998:Glycine