The amino acid compositions of the tryptic, chymotryptic and peptic peptides from the L-2 light chain of rabbit skeletal muscle myosin.
The light chain fraction was separated from rabbit skeletal muscle myosin and four kinds of light chains, L-1, L-2, L-3 and L-4 in the fraction were further isolated by column chromatography using DEAE-cellulose DE-52. After amino-ethylation, the L-2 light chain was digested with trypsin. It was also digested with chymotrypsin and pepsin, respectively, after carboxymethylation. Each of the tryptic, chymotryptic and peptic peptides thus obtained was separated and purified and their amino acid compositions were analyzed.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002918:Chymotrypsin; D046911:Macromolecular Substances; D009132:Muscles; D009218:Myosins; D010434:Pepsin A; D010446:Peptide Fragments / Q000032:analysis*; D011817:Rabbits; D014357:Trypsin
D000596:Amino Acids; D046911:Macromolecular Substances; D010446:Peptide Fragments; D002918:Chymotrypsin; D014357:Trypsin; D010434:Pepsin A; D009218:Myosins