A new microwave device for rapid thermal fixation of the murine brain.
A microwave applicator was developed to provide rapid thermal fixation of enzymes in the murine brain. Although the power output of the device is only 1.3 kW, whole-brain levels of acetyicholine after microwave fixation are comparable to those observed at a power output of 5 kW, and are markedly higher than those obtained by a conventional method (freezing) of fixation.
D000109:Acetylcholine / Q000032:analysis; D000818:Animals; D001921:Brain / Q000201:enzymology; D001923:Brain Chemistry; D005615:Freezing; D006652:Histological Techniques / Q000295:instrumentation*; D006358:Hot Temperature; D051379:Mice; D008872:Microwaves; D051381:Rats; D013696:Temperature
D000109:Acetylcholine