Assay of staphylococcal delta-haemolysin with fish erythrocytes.
Suspensions of erythroctes from certain marine fish (cod, saithe, haddock and mackerel) were 4--16 times more sensitive than human or horse erythrocytes to staphylococcal delta-haemolysin. Cod erythrocytes were lysed rapidly by delta-haemolysin and the titres of haemolysin were independent of the temperatures at which the tests were incubated, over the range 0--37 degrees C. Fish erythrocytes were not lysed by purified alpha- and beta-haemolysins and no synergistic action was found between beta- and delta-haemolysins. In blood agar-overlay plates, the zones of lysis that developed in cod blood agar were larger and clearer than those that developed in human or horse blood agar.
D000818:Animals; D004912:Erythrocytes; D005399:Fishes / Q000097:blood; D006460:Hemolysin Proteins / Q000032:analysis*; D006462:Hemolytic Plaque Technique; D013210:Staphylococcus / Q000276:immunology*; D013211:Staphylococcus aureus / Q000276:immunology*; D013696:Temperature
D006460:Hemolysin Proteins