title: Preparation of lymphoid cells from small specimens of human gastrointestinal mucosa.
abstract: Several methods for the preparation of cell suspensions from human gastrointestinal mucosa were investigated. Satisfactory suspensions were obtained by incubating tissue fragments in a solution of collagenase and hyaluronidase overnight at 4 degrees C followed by 30 minutes at 37 degrees C. The resulting suspension contained large numbers of intact lymphoid cells; in addition, variable amounts of epithelial cells and cell debris were present. A high proportion of the lymphoid cells were shown by immunofluorescence to contain immunoglobulin (mainly IgA). Viability of these cells was demonstrated by dye exclusion, their ability to survive in short-term culture, and their ability to incorporate radio-labelled amino acid into immunoglobulin in vitro.
mesh_terms: D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D003106:Colon / Q000166:cytology; D005455:Fluorescent Antibody Technique; D005753:Gastric Mucosa / Q000166:cytology*; D006801:Humans; D007136:Immunoglobulins / Q000096:biosynthesis; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000166:cytology*; D007421:Intestine, Small / Q000166:cytology; D008214:Lymphocytes / Q000378:metabolism
chemical_list: D007136:Immunoglobulins