A chemical approach to the mechanism of B-lymphocyte activation. I. The pure presentation of haptens does not activate B lymphocytes.
In an attempt to definitively determine whether the pure presentation of haptens (on repetitive, non-metabolizable carriers) to B lymphocytes triggers differentiation to antibody-producing cells, dinitrophenyl and trinitrophenyl groups have been covalently coupled to potentially inert carriers (polymethylmethacrylate, chlorinated polyethylene and cellulose). The resulting conjugates (with three different degrees of hapten substitution) are not immunogenic. It is therefore concluded that the pure presentation of haptens to B lymphocytes does not trigger antibody formation.
D000180:Acrylic Resins / Q000494:pharmacology*; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D000921:Antibody-Producing Cells / Q000187:drug effects; D001402:B-Lymphocytes / Q000276:immunology*; D001666:Binding Sites, Antibody; D002478:Cells, Cultured; D002482:Cellulose / Q000494:pharmacology; D004926:Escherichia coli; D005362:Ficoll / Q000494:pharmacology; D006241:Haptens; D007136:Immunoglobulins; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects*; D051379:Mice; D008815:Mice, Inbred Strains; D011095:Polyethylenes / Q000276:immunology / Q000494:pharmacology*; D011109:Polymethacrylic Acids / Q000276:immunology / Q000494:pharmacology*; D014301:Trinitrobenzenes / Q000276:immunology / Q000494:pharmacology
D000180:Acrylic Resins; D006241:Haptens; D007136:Immunoglobulins; D008070:Lipopolysaccharides; D011095:Polyethylenes; D011109:Polymethacrylic Acids; D014301:Trinitrobenzenes; D005362:Ficoll; D002482:Cellulose