title: ATP-induced conformational changes in the restriction endonuclease from Escherichia coli K-12.
abstract: ATP induces a conformational change in the Escherichia coli K-12 restriction enzyme that allows it to discriminate between unmodified and modified DNA recognition sequences. This conformational change does not require ATP hydrolysis. However, ATP hydrolysis is a requirement for DNA cleavage.
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism / Q000494:pharmacology*; D001665:Binding Sites; D003090:Coliphages; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology; D008745:Methylation; D011487:Protein Conformation / Q000187:drug effects; D012436:S-Adenosylmethionine / Q000378:metabolism; D013379:Substrate Specificity / Q000187:drug effects
chemical_list: D004279:DNA, Viral; D012436:S-Adenosylmethionine; D000255:Adenosine Triphosphate; D004262:DNA Restriction Enzymes