title: Metallic deposition in specimens presenting cavities using the sputter coater.
abstract: Local charging effects in small biological cavities during SEM observation are apparent, even after sputter coating. Using an experimental model, the thickness of metallic coating at the bottom of cylindrical cavities has been measured and found to be less than 50% the thickness on the surface, depending on the relation of diameter to depth of the cylinder.
mesh_terms: D000818:Animals; D001921:Brain / Q000033:anatomy & histology*; D003720:Densitometry; D006652:Histological Techniques / Q000295:instrumentation; D008670:Metals; D008855:Microscopy, Electron, Scanning; D008954:Models, Biological; D051381:Rats
chemical_list: D008670:Metals