A method for preparing leucocyte depleted erythrocytes from rat blood.
By centrifuging heparinised rat blood 2 or 3 times through double discontinuous Ficoll--Hypaque density density gradients it is possible to achieve over 99.8% depletion of leucocytes and to retrieve nearly all the erythrocytes and leucocytes in the original specimen.
D000818:Animals; D002469:Cell Separation; D002499:Centrifugation, Density Gradient; D004912:Erythrocytes / Q000276:immunology*; D007158:Immunologic Techniques; D007962:Leukocytes / Q000276:immunology*; D051381:Rats