[Production of colicins V and V2 in vitro and in vivo. Study of their inhibitory action on phagocytosis by peritoneal macrophages].
In recent years, a possible relationship between pathogenicity and colicinogeny in some Escherichia coli colicin V-producing strains had been inferred. In our laboratory, we have elaborated a simple in vitro method for the production of colicin V free of large, non dialyzable macromolecules and presumably of bacterial endotoxin. This allows study of the effects of colicin V in vivo without an undesirable added physiological response of the experimental animal to endotoxin. All the Col V+ strains we have studied displayed a greater ability to survive in the peritoneal cavity of mice than the Col V- strains. Also, we have detected colicin V in peritoneal fluids of agonizing mice injected with Col V+ strains. Phagocytosis by peritoneal macrophages seemed to be inhibited in vitro in the presence of colicin V. Colicin V is not toxic in vivo in low concentration, after intraperitoneal or intravenous injection but it may favor the multiplication and the invasiveness of the strains that produce it.
D000818:Animals; D001202:Ascitic Fluid / Q000166:cytology; D003087:Colicins / Q000096:biosynthesis* / Q000494:pharmacology; D004926:Escherichia coli / Q000378:metabolism*; D004927:Escherichia coli Infections / Q000382:microbiology*; D066298:In Vitro Techniques; D008264:Macrophages / Q000187:drug effects*; D008722:Methods; D051379:Mice; D010587:Phagocytosis / Q000187:drug effects*
D003087:Colicins
Production de colicines V et V2 in vitro et in vivo. étude de leur action inhibitrice sur la phagocytose par des macrophages péritonéaux.