The role of misrepair processes in the isolation of new types of streptomycin-resistant mutants in Escherichia coli.
Treatment of Escherichia coli cells with ethylmethanesulfonate followed by a prolonged delay for phenotypic expression allows to select new types of streptomycin-resistant mutants, which are double mutants with one change resulting from base mispairing and the second one from misrepair. The error-prone recA-dependent pathway is involved in this misrepair, as evidenced by the fact that recA strains do not provide double mutants.
D004260:DNA Repair; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects / Q000302:isolation & purification; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D005796:Genes; D009154:Mutation; D011995:Recombination, Genetic; D013307:Streptomycin / Q000494:pharmacology*; D013997:Time Factors
D005020:Ethyl Methanesulfonate; D013307:Streptomycin