Demonstration of a tertiary interaction in solution between the extra arm and the D-stem in two different transfer RNA's by NMR.
According to the X-ray structure of yeast tRNAPhe at 2.5 A resolution, a hydrogen bond is formed between m7G46 and G22. By removal of this m7G46-residue we demonstrate that this interaction is present in solution as well. Comparison of the 1H 360 MHz NMR spectra of intact yeast tRNAPhe and its m7G-excised derivative locates the position of this tertiary H-bond at 12.5 ppm downfield from DSS. Additional evidence for the presence of this interaction in solution comes from a comparison of 1H NMR spectra of E. coli tRNAf1Met and E. coli tRNAf3Met, which differ only in a single position in the extra arm. In tRNAf3Met residue 47 is a m7G-residue, whereas in tRNAf3Met it is A, resulting in the absence of the m7G47 - G23 - C13 triple interaction, characteristic of tRNAf1Met. The resonance position of this tertiary interaction in tRNAf1Met is located around -13.6 ppm, a chemical shift difference of 1.1 ppm with respect to the position observed for tRNAPhe. The origin of this chemical shift difference is discussed in relation to the structure of their respective augmented D-helices.
D004926:Escherichia coli; D006860:Hydrogen Bonding; D009682:Magnetic Resonance Spectroscopy; D009239:N-Formylmethionine; D009690:Nucleic Acid Conformation; D010649:Phenylalanine; D012329:RNA, Bacterial; D012343:RNA, Transfer; D012441:Saccharomyces cerevisiae; D012996:Solutions
D012329:RNA, Bacterial; D012996:Solutions; D009239:N-Formylmethionine; D010649:Phenylalanine; D012343:RNA, Transfer