Cytoskeletal filaments of heart conducting system localized by antibody against a 55,000 dalton protein.
Cow heart conducting cells characteristically contain cytoplasmic intermediate-sized filaments. We report here the preparation of a specific antibody to a 55,000 dalton protein of isolated cow Purkinje fibres. Confirmation has been obtained that these filaments consist of the 55,000 dalton protein, using the indirect immunofluorescence technique. Cross-reaction is seen with vascular endothelium and smooth muscle cells of various origin, suggesting close identity of different types of intermediate-sized filaments.
D000818:Animals; D002417:Cattle; D003593:Cytoplasm / Q000032:analysis / Q000648:ultrastructure*; D005455:Fluorescent Antibody Technique; D006329:Heart Conduction System / Q000032:analysis / Q000648:ultrastructure*; D009124:Muscle Proteins / Q000032:analysis; D011690:Purkinje Fibers / Q000648:ultrastructure
D009124:Muscle Proteins