Application of higher derivative techniques to analysis of high-resolution thermal denaturation profiles of reassociated repetitive DNA.
We have analyzed high-resolution denaturation profiles of reassociated repetitive DNA sequences by using a combination of higher derivative analysis and curve-fitting techniques. Procedures originally used for resolution of components in complex absorption spectra were found to be applicable to high-resolution analysis of melting profiles of reassociated repetitive DNA sequences from pea DNA. Under conditions that eliminate the base composition effect on thermal stability (2.4 M tetraethylammonium chloride), such an anlysis can distinquish "thermal classes" of repetitive DNA duplexes exhibiting different amounts of base pair mismatch. Only a single thermal class is observed in reassociated Escherichia coli DNA whereas at least five classes can be reproducibly distinguished in pea and mung bean DNAs.
D001483:Base Sequence; D003201:Computers; D004247:DNA / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli; D006358:Hot Temperature; D006860:Hydrogen Bonding; D009691:Nucleic Acid Denaturation; D010944:Plants; D013757:Tetraethylammonium Compounds / Q000494:pharmacology
D004269:DNA, Bacterial; D013757:Tetraethylammonium Compounds; D004247:DNA