[Detection of rubella virus antibodies in single radial haemolysis-test (author's transl)].
Different kinds of erythrocytes (human, pigeon, chicken and sheep) and complement (guinea pig, rabbit and rat) were tested in rubella single radial hemolysis-test (SRHT, hemolysis-in-gel = HIG-test) in order to detect IgG and IgM antibodies. Experiments showed that sera which were used as complement, contained antibodies against different kinds of erythrocytes. If complement from rabbit and rat should be used, an adsorption will be necessary. For serological surveillance sheep and chicken erythrocytes in connection with guinea pig serum as complement were best suited. Positive results were also obtained with pigeon erythrocytes whereas human RBC gave negative reactions. A detection of IgM antibodies in the SRHT was not possible.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D002645:Chickens; D003165:Complement System Proteins; D004912:Erythrocytes / Q000276:immunology; D006168:Guinea Pigs / Q000276:immunology; D006462:Hemolytic Plaque Technique; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D012412:Rubella virus / Q000276:immunology*; D012756:Sheep
D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D003165:Complement System Proteins
Nachweis von Antikörpern gegen das Rötelnvirus im Single Radial Haemolysis-Test.