title: Comparison of the histamine hypersensitivity and the Limulus amoebocyte lysate tests for endotoxin activity.
abstract: The histamine hypersensitivity test and the Limulus amoebocyte lysate test were compared for their effectiveness to quantitate endotoxin activity. The two tests compared favorably in all the trials, except with a sample of endotoxin from Brucella abortus that gave a positive Limulus amoebocyte lysate test at a concentration of 0.001 microgram, while failing to sensitize mice to histamine at a dose of 16 microgram per mouse. The Limulus amoebocyte lysate test was more sensitive than the histamine hypersensitivity test.
mesh_terms: D000818:Animals; D004342:Drug Hypersensitivity; D004731:Endotoxins / Q000032:analysis*; D006632:Histamine / Q000276:immunology*; D007158:Immunologic Techniques; D008033:Limulus Test; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains
chemical_list: D004731:Endotoxins; D006632:Histamine