The proton exchange of the pro-S hydrogen atom at C-1 in dihydroxyacetone phosphate and D-fructose 1,6-bisphosphate catalysed by class-I and class-II aldolases.
The efficacy of class-I and class-II aldolases in catalysing the C-1 proton exchange in fructose 1,6-bisphosphate and dihydroxyacetone phosphate was investigated. The rate of this reaction was at least two orders of magnitude slower in class-II than in the class-I aldolases. It is suggested that this difference reflects the formation of different intermediates in the reactions catalysed by the two classes of aldolase.
D004099:Dihydroxyacetone Phosphate / Q000378:metabolism*; D005634:Fructose-Bisphosphate Aldolase / Q000378:metabolism*; D005636:Fructosephosphates / Q000378:metabolism*; D001411:Geobacillus stearothermophilus / Q000201:enzymology; D011522:Protons; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D014306:Trioses / Q000378:metabolism*
D005636:Fructosephosphates; D011522:Protons; D014306:Trioses; D004099:Dihydroxyacetone Phosphate; D005634:Fructose-Bisphosphate Aldolase