Selective binding of antibody against gizzard 10-nm filaments to different cell types in myogenic cultures.
Antibody against the intermediate-sized filaments from gizzard smooth muscle was used to determine the presence or absence of reacting 10-nm filaments in different cell types. The antibody against gizzard 10-nm filaments reacted with filaments in cultured smooth muscle cells, skeletal myotubes and postmitotic skeletal myoblasts. It did not bind to the 10-nm filaments present in replicating presumptive myoblasts and fibroblasts, or the 10-nm filaments in spinal ganglion cells.
D000818:Animals; D001666:Binding Sites, Antibody; D002478:Cells, Cultured; D002642:Chick Embryo; D003429:Cross Reactions; D005347:Fibroblasts / Q000276:immunology; D005455:Fluorescent Antibody Technique; D005727:Ganglia, Spinal / Q000276:immunology; D005895:Gizzard, Avian / Q000276:immunology*; D009130:Muscle, Smooth / Q000276:immunology*; D009132:Muscles / Q000276:immunology*