Inactivation of bacteriophages by protein E, a new major membrane protein isolated from an Escherichia coli mutant.
Pure protein E, obtained after diethylaminoethyl-cellulose chromatography of ethylenediaminetetraacetic acid-Triton X-100-solubilized outer membrane proteins of Escherichia coli strain JF694, inactivated bacteriophage K3. Lipopolysaccharide enhanced bacteriophage inactivation. Antibody prepared against purified protein E protected bacteriophage K3 from inactivation by protein E. Bacteriophage K3 used a major outer membrane protein, protein II*, as part of its receptor. We conclude that proteins E and II* have a common region which interacts with bacteriophage K3. Protein E also inactivated two recently described bacteriophages, TC45 and TC23, that use protein E as at least part of their receptor.
D000956:Antigens, Viral; D001426:Bacterial Proteins / Q000276:immunology / Q000494:pharmacology*; D003090:Coliphages / Q000187:drug effects* / Q000502:physiology; D004926:Escherichia coli; D007106:Immune Sera / Q000494:pharmacology; D007700:Kinetics; D008070:Lipopolysaccharides / Q000494:pharmacology; D008565:Membrane Proteins / Q000276:immunology / Q000494:pharmacology*; D011991:Receptors, Virus / Q000502:physiology*
D000956:Antigens, Viral; D001426:Bacterial Proteins; D007106:Immune Sera; D008070:Lipopolysaccharides; D008565:Membrane Proteins; D011991:Receptors, Virus