[Heat induced structural transformations in Escherichia coli membranes in the presence of Ca2+].
Using an uncharged hydrophobic fluorescent probe 4-dimethylaminochalkon (DMC) the thermotropic behaviour of cytoplasmic E. coli membranes in the presence of Ca2+ cations was investigated. DMC appeared to be very sensitive in detection of temperature induced phase transitions in E. coli membrane preparations. It was found unexpectedly that Ca2+ does not increased the phase transition temperature of membrane preparations, compared to the values calculated for the membranes in tris-HC1-buffer. The obtained results are briefly discussed.
D002118:Calcium / Q000494:pharmacology*; D002462:Cell Membrane / Q000187:drug effects*; D004577:Electron Probe Microanalysis; D004926:Escherichia coli / Q000648:ultrastructure*; D006358:Hot Temperature; D013050:Spectrometry, Fluorescence
D002118:Calcium
Strukturnye perestroĭki v membranakh Escherichia coli v prisutstvii kationov Ca2+, indutsirovannye nagrevaniem.