Reliable technique of preparation of ultrathin sections of tissue infected with mycobacteria.
A technique of preparation of ultrathin sections of liver tissue infected with mycobacteria, concretely of Kupffer cells containing Mycobacterium avium, was described. Satisfactory preservation of the ultrastructure of both liver and mycobacteria was achieved by a modification of sampling, dehydration and Vestopal-embedding procedures.
D000818:Animals; D006652:Histological Techniques; D007728:Kupffer Cells / Q000382:microbiology / Q000648:ultrastructure; D008099:Liver / Q000382:microbiology / Q000648:ultrastructure*; D009161:Mycobacterium / Q000648:ultrastructure*; D009164:Mycobacterium Infections / Q000382:microbiology / Q000473:pathology*; D009162:Mycobacterium avium / Q000648:ultrastructure*; D011817:Rabbits