Uraemic toxins of high molecular weight inhibiting human mononuclear phagocytes cultured in vitro.
Uraemic plasma and normal serum were separated into fractions of different molecular sizes by means of molecular filtration through Pellicon filters and liquid chromatography on Sephadex G-15. The amount of substance was considerably higher in uraemic fractions than in comparable normal fractions. When normal human mononuclear phagocytes were cultured in the presence of the uraemic fraction containing the largest molecules, detachment of phagocytes from the glass coverslips was significantly increased. The digestion capacity of the remaining cells was decreased. The results indicate that substances in uraemic plasma responsible for the impaired function of human mononuclear phagocytes cultured in vitro consist of molecules of a molecular weight higher than 10 000.
D002176:Candida albicans; D002478:Cells, Cultured; D002850:Chromatography, Gel; D006801:Humans; D008851:Micropore Filters; D008970:Molecular Weight; D009000:Monocytes / Q000187:drug effects*; D010587:Phagocytosis / Q000187:drug effects*; D014118:Toxins, Biological / Q000494:pharmacology*; D014511:Uremia / Q000097:blood*
D014118:Toxins, Biological