Lysosomal enzyme release from guinea pig alveolar cells: biochemical and morphologic observations.
Cells lavaged from guinea pig lungs selectively release the lysosomal enzymes beta-glucuronidase and lysozyme during the process of phagocytosis. The total enzymatic activity of beta-glucuronidase was elevated in zymosan-exposed compared with non-exposed cells. The divalent cation carrying ionophore A23187 did not stimulate lysosomal enzyme release. Transmission and scanning electron micrographs demonstrate particle uptake and are consistent with enzyme release.
D000818:Animals; D000001:Calcimycin / Q000494:pharmacology; D002118:Calcium / Q000502:physiology; D003571:Cytochalasin B / Q000494:pharmacology; D005260:Female; D005966:Glucuronidase / Q000378:metabolism*; D006168:Guinea Pigs; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008247:Lysosomes / Q000201:enzymology; D008297:Male; D009113:Muramidase / Q000378:metabolism*; D009504:Neutrophils / Q000201:enzymology*; D010587:Phagocytosis; D011650:Pulmonary Alveoli / Q000201:enzymology* / Q000648:ultrastructure
D000001:Calcimycin; D003571:Cytochalasin B; D007770:L-Lactate Dehydrogenase; D009113:Muramidase; D005966:Glucuronidase; D002118:Calcium