Platelet factor VIII-related antigen: studies in vivo after transfusion in patients with von Willebrand disease.
Four unrelated patients with a severe form of von Willebrand disease showed no detectable factor VIII-related antigen (VIIIR:AG) in either their plasma or their platelets. They received cryoprecipitate infusions, three patients in a single injection each and one every day for 9 days before and after surgery. Platelet VIIIR:Ag was studied at different times during and after transfusion using electroimmunoassay of platelet extracts and electron microscopy of the platelets incubated with anti-VIIIR:Ag antibodies coupled to peroxidase. No VIIIR-Ag was detected in or around the patients' platelets, although this antigen was detected in the circulating blood. These results that there was no VIIIR:Ag uptake from the plasma by the platelets and that platelet VIIIR:Ag came from megakaryocytes.
D000328:Adult; D000941:Antigens; D001780:Blood Coagulation Tests; D001792:Blood Platelets / Q000276:immunology*; D001803:Blood Transfusion; D011232:Chemical Precipitation; D002648:Child; D003450:Cryoglobulins / Q000627:therapeutic use; D005169:Factor VIII / Q000276:immunology*; D005260:Female; D006801:Humans; D007123:Immunoelectrophoresis, Two-Dimensional; D007124:Immunoenzyme Techniques; D008297:Male; D013997:Time Factors; D014842:von Willebrand Diseases / Q000276:immunology*
D000941:Antigens; D003450:Cryoglobulins; D005169:Factor VIII