Role of T and adherent cells in in vitro response of nude mouse spleen cells to bacterial lipopolysaccharide.
Thy 1.2-positive cells and adherent cells were depleted from nude mouse spleen cell suspensions by treatment with anti-Thy 1.2 antisera plus complement and by passage through Sephadex G-10 column, respectively. The results of 3H-TdR uptake and appearance of IgM containing cells induced in vitro by lipopolysaccharide (LPS) were similar in both untreated and depleted spleen cells. Our findings confirm T and adherent cell independence of LPS response in nude mice.
D000818:Animals; D002448:Cell Adhesion; D004926:Escherichia coli / Q000276:immunology; D007075:Immunoglobulin M; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010581:Peyer's Patches / Q000276:immunology; D013154:Spleen / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*; D013936:Thymidine / Q000378:metabolism
D007075:Immunoglobulin M; D008070:Lipopolysaccharides; D013936:Thymidine