title: Effect of R-plasmid RP1 and nutrient depletion on the gross cellular composition of Escherichia coli and its resistance to some uncoupling phenols.
abstract: The resistance of Escherichia coli batch cultures depleted of carbon (C-dep), magnesium (Mg-dep), or phosphate (P-dep) against low concentrations of 3-chlorophenol, 4-chlorophenol, or 2-phenoxyethanol varied. C-dep cultures were always significantly more sensitive than Mg-dep or P-dep cultures. The presence of R-plasmid RP1 increased the sensitivity of C-dep cultures to 3- and 4-chlorophenol, yet had little effect on those cultured depleted in magnesium or phosphate ions. Cultures with R-plasmid RP1 had increased levels of beta-polyhydroxybutyrate irrespective of the nature of the depleting nutrient. P-dep bacteria had less than one-third of the phospholipid of other cell types, this deficiency being compensated for by increases in fatty acid and neutral lipid content. The reduction in phospholipid content of P-dep cultures was entirely accounted for by decreased diphosphatidylglycerol and phosphatidylethanolamine levels in these cells.
mesh_terms: D002244:Carbon / Q000378:metabolism; D002733:Chlorophenols / Q000494:pharmacology*; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000032:analysis / Q000187:drug effects* / Q000235:genetics; D005026:Ethylene Glycols / Q000494:pharmacology*; D006885:Hydroxybutyrates / Q000032:analysis; D008274:Magnesium / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D010743:Phospholipids / Q000032:analysis; D011815:R Factors
chemical_list: D002733:Chlorophenols; D005026:Ethylene Glycols; D006885:Hydroxybutyrates; D010710:Phosphates; D010743:Phospholipids; D002244:Carbon; C005398:phenoxyethanol; D008274:Magnesium