title: Radioimmunoassay and automated enzyme immunoassay for the determination of thyroxine.
abstract: The EMIT homogeneous enzyme immunoassay for the determination of thyroxine (T4) was adapted to an automatic analyser (Type Kem-O-Mat) and compared with a conventional radioimmunoassay. Sera from 92 patients whose thyrometabolic status was being examined, and control sera with 3 different T4 concentrations were analysed by both methods. Day-to-day analyses of the control sera gave variation coefficients between 6 and 10% for both methods. The mean value for T4 in the patients sera was 111 nmol/l (8.6 microgram/dl) determined by the enzyme immunoassay and 109 nmol/l (8.5 microgram/dl) determined by the radioimmunoassay; the correlation coefficient was r = 0.9.
mesh_terms: D001322:Autoanalysis; D006801:Humans; D007124:Immunoenzyme Techniques; D011863:Radioimmunoassay; D013223:Statistics as Topic; D013974:Thyroxine / Q000032:analysis*
chemical_list: D013974:Thyroxine