Immunofluorescence studies on formalin-fixed and paraffin-embedded material.
In an attempt to apply immunomorphologic techniques to formalin-fixed material, various tissues were studied by indirect immunofluorescence. To minimize nonspecific background staining, formalin-fixed paraffin sections were treated with pronase prior to incubation with the antisera. This technique was published by Huang in 1975 to study hepatitis B core and surface antigen in formalin-fixed liver-tissue. Our examination demonstrate that this technique might enable the morphologist to identify immunoglobulins in formalin-fixed tissues and so to get new criteria for their diagnosis.
D000328:Adult; D001943:Breast Neoplasms / Q000276:immunology; D002675:Child, Preschool; D005260:Female; D005455:Fluorescent Antibody Technique; D005557:Formaldehyde; D006652:Histological Techniques; D006801:Humans; D016403:Lymphoma, Large B-Cell, Diffuse / Q000276:immunology; D008297:Male; D010019:Osteomyelitis / Q000276:immunology; D010232:Paraffin; D012488:Salpingitis / Q000276:immunology
D005557:Formaldehyde; D010232:Paraffin