[Study of the structure of tRNA by the energy migration method using fluorescent labels covalently bound to specific tRNA loci].
Optical and fluorescent characteristics of fluorescein covalently attached to 3'-end of tRNAFhe and X-nucleotide in the extra arm of several species of tRNA from E. coli have been studied. The probe is shown to be a sensitive factor indicating the conformational change of tRNA induced by Mg2+ and Na+ ions. By measuring the extent of energy transfer the distances between the fluorescent probe attached to 3'-terminus and X-nucleotide of tRNA and specific binding site of ethidium bromide on tRNA were determined to be 40.5 A and 32.5 A, respectively. The distances measured are in good agreement with the NMR spectroscopy data showing that the specific binding site for ethidium bromide on tRNA is localised near the sixth base pair of the acceptor stem.
D000604:Amino Acyl-tRNA Synthetases; D001665:Binding Sites; D055598:Chemical Phenomena; D002621:Chemistry; D004735:Energy Transfer; D004926:Escherichia coli / Q000032:analysis; D004996:Ethidium; D005452:Fluoresceins; D005998:Glycine; D008274:Magnesium; D008722:Methods; D009690:Nucleic Acid Conformation; D012329:RNA, Bacterial; D012343:RNA, Transfer; D012270:Ribosomes; D012964:Sodium; D013050:Spectrometry, Fluorescence
D005452:Fluoresceins; D012329:RNA, Bacterial; D012343:RNA, Transfer; D012964:Sodium; D000604:Amino Acyl-tRNA Synthetases; D004996:Ethidium; D008274:Magnesium; D005998:Glycine
Issledovanie struktury tRNK metodom migratsii énergii s ispol'zovaniem fluorestsentnykh metok, kovalentno prisoedinennykh k spetsificheskim mestam tRNK.