Light- and electron microscopic localization of vasopressin or a vasopressin-like substance in the neurons of the rat suprachiasmatic nucleus.
In the suprachiasmatic nucleus of the rat light microscopic immunostaining for vasopressin reveals a distribution pattern of the immunoreactive material different from that known for the supraoptic nucleus. Among non-stained neurons positive-reacting perikarya display a cap- or tip-like labeling. The area of the suprachiasmatic nucleus is marked by delicate vasopressin-positive fibers. At the ultrastructural level the reaction product, after incubation with anti-vasopressin, is localized in small elementary granules unevenly distributed over the cytoplasm. Groups of axons containing specifically labeled granules contact non-reacting fibers.
D000818:Animals; D005260:Female; D006651:Histocytochemistry; D007031:Hypothalamus / Q000032:analysis*; D007158:Immunologic Techniques; D008297:Male; D008854:Microscopy, Electron; D051381:Rats; D013495:Supraoptic Nucleus / Q000032:analysis* / Q000648:ultrastructure; D014667:Vasopressins / Q000032:analysis*
D014667:Vasopressins