Omission of pepsin from simulated gastric fluid in evaluating activated charcoals as antidotes.
Although simulated gastric fluid USP calls for 3.2 g of pepsin/liter, most researchers omit pepsin when evaluating adsorbents. The present results show that, although pepsin adsorbs strongly to activated charcoal, it does not interfere significantly with the adsorption of a typical drug like sodium salicylate. Therefore, its omission is justified. Gastric mucin also had almost no effect on salicylate adsorption.
D000327:Adsorption; D000931:Antidotes; D001667:Binding, Competitive; D002606:Charcoal / Q000494:pharmacology*; D005750:Gastric Juice / Q000032:analysis / Q000201:enzymology*; D005752:Gastric Mucins / Q000032:analysis; D010434:Pepsin A / Q000032:analysis; D012980:Sodium Salicylate / Q000032:analysis
D000931:Antidotes; D005752:Gastric Mucins; D002606:Charcoal; D010434:Pepsin A; D012980:Sodium Salicylate