[Competence in Escherichia coli cells. V. Interaction of the competence factor with the cells].
Some trends of the interaction of the competence factor with the cells are described on the model of phage lambda transfection. It is shown that this process depends on the composition of the recipients growth media, the temperature regime in the interaction processand on the concentration of the competence factor and the recipient cells. The preliminary treatment of the recipient by EDTA (0.0001 M), CaCl2 (less than 0.02 M), MgCl2 (less than 0.01 M), and NaCl (less than 0.01 M) resulted in the increaze of their sensitivity to subsequent treatment by the factor. Data are obtained demonstrating that after the treatment of cells by the competence factor their sensitivity to decreased CaCl2 concentration is increasing.
D002122:Calcium Chloride / Q000494:pharmacology; D003090:Coliphages / Q000235:genetics; D003470:Culture Media; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D013696:Temperature; D013997:Time Factors; D014162:Transfection / Q000187:drug effects; D014170:Transformation, Genetic / Q000187:drug effects
D003470:Culture Media; D004269:DNA, Bacterial; D002122:Calcium Chloride
Kompetentnost' u kletok Escherichia coli. Soobshchenie V. Vzaimodeĭstvie faktora kompetentnosti s kletkami.