Enzyme immunoassay for hepatitis B and its comparison to other methods.
A solid-phase enzyme immunoassay for the qualitative detection of hepatitis B surface antigenemia is presented and the results compared to the counter-immunoelectrophoresis and radioimmunoassay methods for detection of the same antigen. The enzyme-antibody conjugate was prepared from horseradish peroxidase coupled with antibodies to three sub-types of hepatitis B virus. Polystyrene plastic tubes were used as the solid-phase support. The results of the enzyme immunoassay compare favorably with radioimmunoassay and exceed counterimmunoelectrophoresis in sensitivity.
D003378:Counterimmunoelectrophoresis; D006514:Hepatitis B Surface Antigens / Q000032:analysis*; D006801:Humans; D007124:Immunoenzyme Techniques; D011863:Radioimmunoassay
D006514:Hepatitis B Surface Antigens