Increased production of PGI2-like activity by frozen-thawed rat aorta.
The effects of storage conditions, temperature, and time on the ability of the rat thoracic aorta to produce a platelet aggregation inhibitor were investigated. Aortic fragments were incubated in Tris buffer, aliquots of which were then tested for their ability to inhibit ADP-induced human platelet aggregation. The incubation fluid of samples that had been soaked in Tris buffer at 4 degrees C for 24 hours contained no inhibitor activity, whereas the incubation fluid of similar samples that had been kept at 4 degrees C but not soaked in buffer contained comparable inhibitor activity as that of fresh samples. The incubation fluid of samples that had been kept at -20 degrees C or -80 degrees C contained greater inhibitor activity than that of fresh samples, and was maintained in -20 degrees C samples for 7 days, and -80 degrees samples for 28 days. The aortic inhibitor had similar properties as PGI2.
D000818:Animals; D001013:Aorta, Thoracic / Q000378:metabolism / Q000648:ultrastructure; D001158:Arteries / Q000378:metabolism*; D001241:Aspirin / Q000494:pharmacology; D002021:Buffers; D003402:Creatine Kinase / Q000378:metabolism; D011464:Epoprostenol / Q000096:biosynthesis*; D005260:Female; D005615:Freezing; D009130:Muscle, Smooth / Q000378:metabolism / Q000648:ultrastructure; D010974:Platelet Aggregation / Q000187:drug effects; D011453:Prostaglandins / Q000096:biosynthesis*; D051381:Rats; D013048:Specimen Handling; D013696:Temperature; D013997:Time Factors; D014021:Tissue Preservation
D002021:Buffers; D011453:Prostaglandins; D011464:Epoprostenol; D003402:Creatine Kinase; D001241:Aspirin