Growth of animal cells on glass fiber filters and their utilization for biosynthetic analysis.
HeLa and L-M cells can be effectively grown directly on glass fiber filters to yield replicate cultures that allow easy analysis of biosynthetic capabilities through measurement of radioactive precursor uptake and incorporation. The glass fiber filters are superior to glass cover slips, growth in scintillation vials, and growth on Millipore filters in the ease of handling during experimental treatment and in the amount of radioactivity incorporated during the labeling period. These parameters are experimentally established and a typical application of the procedure that demonstrates the hydroxyurea inhibition of DNA synthesis is presented.
D002452:Cell Count; D002455:Cell Division; D002478:Cells, Cultured / Q000378:metabolism; D003584:Cytological Techniques / Q000295:instrumentation*; D004247:DNA / Q000096:biosynthesis; D006367:HeLa Cells; D006918:Hydroxyurea / Q000494:pharmacology; D014176:Protein Biosynthesis; D012313:RNA / Q000096:biosynthesis; D013936:Thymidine / Q000378:metabolism
D012313:RNA; D004247:DNA; D013936:Thymidine; D006918:Hydroxyurea