[Evaluation of procedures for hygienic disinfection of hands: comparison of two methods, for artificially contaminating hands and use of an automatic colony-counter (author's transl)].
Testing procedures for the Hygienic Disinfection of hands, two kinds of artificial contamination have been compared: Application of one drop of bacterial suspension onto each fingertip as described in the DGHM-"Richtlinien" and immersion of hands as described in our previous papers. When using our method, fingertips released higher numbers of testbacteria. Since pre- as well as post-values were elevated, disinfection-tests applying one of each contamination method resulted in log10 reductions which did not differ significantly. Using an automated scanning colony counter we were able to save 2/3 of time otherwise required for manual counts. Up to 10(2) colonies per plate manual counts were practically identical with the counters results, whereas with higher colony-numbers the counter showed an increasing tendency to under-estimate the numbers of colonies. Since colonies of skin-staphylococci are not recognised by the automated counter, we cultured our rinsing-fluids on nutrient agar containing 0.05% of sodium-desoxycholate in order to inhibit the autochthonous skin flora. This supplement influenced the colony counts of our test-bacterium to a very minor degree only, the reduction factors were not at all altered.
D001431:Bacteriological Techniques; D004202:Disinfectants; D004203:Disinfection / Q000592:standards; D004926:Escherichia coli / Q000302:isolation & purification*; D000431:Ethanol; D006225:Hand; D006801:Humans; D012867:Skin / Q000382:microbiology; D013242:Sterilization / Q000592:standards
D004202:Disinfectants; D000431:Ethanol
Wertbestimmung von Verfahren für die Hygienische Händedesinfektion: Vergleich zweier Kontaminationsmethoden und Verwendung eines automatischen Kolonienzählgerätes.