Synchronization of Drosophila cells in culture.
We synchronized Drosophila cell lines (Schneider's line 2 and Kc) by allowing the cells to enter the stationary phase of growth and then diluting them into fresh culture medium. The cells of both cell lines entered S phase, after an 8- to 14-hr delay, in a state of partial synchrony; 60 to 80% of the cell population accumulated in S phase. Measurements of the cell cycle phases of Schneider's line 2 cells (S = 14 to 16 hr; G2 = 6 to 8 hr; M = 0.4 hr) were similar to those of Kc cells.
D002453:Cell Cycle; D002455:Cell Division; D002460:Cell Line; D003470:Culture Media; D003584:Cytological Techniques; D004247:DNA / Q000096:biosynthesis; D004330:Drosophila; D007399:Interphase
D003470:Culture Media; D004247:DNA