[Formation of recombinants of F' plasmids in recombination-defective E. coli cells and their properties].
As a result of mating of cells carrying plasmid F' lac with cells carrying plasmid F'his merodiploids carrying plasmid complex F'lacF'his were isolated. The plasmid genes of the isolated merodiploids are donated together into the recipient cells and are eliminated jointly from host-cells both spontaneously and by acridine orange. As the formation of the plasmid complex took place in E. coli cells carrying mutation in rec A gene the recombination of plasmids F' is supposed to take place in the absence of the product of gene rec. A.
D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D010957:Plasmids; D011995:Recombination, Genetic
Formirovanie rekombinantov plazmid F' v rekombinatsionno-defektnykh kletkakh E. coli i ikh svoĭstva.