The ligand-induced solubility shift in salting out chromatography: a new affinity technique, demonstrated with phenylalanyl- and isoleucyl-tRNA synthetase from baker's yeast.
D000604:Amino Acyl-tRNA Synthetases / Q000302:isolation & purification*; D000645:Ammonium Sulfate; D002846:Chromatography, Affinity / Q000379:methods; D007533:Isoleucine-tRNA Ligase / Q000302:isolation & purification*; D008024:Ligands; D009994:Osmolar Concentration; D010652:Phenylalanine-tRNA Ligase / Q000302:isolation & purification*; D011485:Protein Binding; D012343:RNA, Transfer / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology; D012995:Solubility
D008024:Ligands; D012343:RNA, Transfer; D000604:Amino Acyl-tRNA Synthetases; D010652:Phenylalanine-tRNA Ligase; D007533:Isoleucine-tRNA Ligase; D000645:Ammonium Sulfate