title: Primary Klebsiella identification with MacConkey-inositol-carbenicillin agar.
abstract: MacConkey-inositol-carbenicillin agar has successfully been used as a primary selective medium for Klebsiella enumeration. With pure cultures, nearly 100% recovery of Klebsiella was observed by membrane filtration. With environmental samples using membrane filtration, 95% of typical pink- to red-colored colonies were verified as Klebsiella, as opposed to only 1% of yellow background colonies. Recovery of Klebsiella on MacConkey-inositol-carbenicillin agar was as good or better than on mEndo agar LES (Difco Laboratories). Recovery and percent colony confirmation with MacConkey-inositol-carbenicillin agar were greater than for other proposed Klebsiella selective media.
mesh_terms: D000362:Agar; D002228:Carbenicillin; D005618:Fresh Water; D006801:Humans; D007294:Inositol; D007709:Klebsiella / Q000302:isolation & purification*; D007710:Klebsiella Infections / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000302:isolation & purification; D012722:Sewage; D013045:Species Specificity; D014871:Water Microbiology
chemical_list: D012722:Sewage; D007294:Inositol; D000362:Agar; D002228:Carbenicillin