A technique for the immunohistochemical localization of prostaglandin E.
Prostaglandin E (PGE) has been localized via the unlabeled antibody technique in freeze-dried and ethanol-fixed cryostat sections. Discrete perivascular and stromal localization was present in the uterus prepared by the method presented, but not in classically fixed specimens. Absorption of the anti-PGE by addition of free PGE was ineffective; whereas, removal of PGE-reactive antibodies from the anti-serum was effectively accomplished with an Affigel-101-PGE immunoadsorbant column.
D000818:Animals; D005260:Female; D005404:Fixatives; D006652:Histological Techniques; D007124:Immunoenzyme Techniques; D011458:Prostaglandins E / Q000032:analysis* / Q000276:immunology; D051381:Rats; D014599:Uterus / Q000166:cytology*
D005404:Fixatives; D011458:Prostaglandins E