title: Beneficial effects of catalase or pyruvate in a most-probable-number technique for the detection of Staphylococcus aureus.
abstract: The effects of the addition of catalase (EC 1.11.1.6) or pyruvate on the enumeration of Staphylococcus aureus in Trypticase soy broth with 10% NaCl were examined using a most-probable-number technique. Addition of catalase or pyruvate to the broth increased enumeration of all heat-stressed S. aureus strains tested. Increases were also observed with nonstressed cells. Catalase and pyruvate were similarly effective when added to Trypticase soy broth-10% NaCl in enumerating staphylococci naturally present in low-temperature-rendered ground-beef samples.
mesh_terms: D001431:Bacteriological Techniques; D002374:Catalase / Q000494:pharmacology*; D003470:Culture Media; D005506:Food Contamination; D005516:Food Microbiology; D006358:Hot Temperature; D011773:Pyruvates / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000302:isolation & purification*
chemical_list: D003470:Culture Media; D011773:Pyruvates; D002374:Catalase