Use of ELISA for measuring immunoconglutinins.
An enzyme-linked immunosorbent assay (ELISA) for detection of immunoconglutinins (IKs) has been developed. IKs are allowed to bind to solid-phase C3 and are then assayed using alkaline phosphatase coupled to anti-IgG or anti-IgM. IK activity was detected in some patients' sera up to 50,000-fold dilution. Significant correlation (r = 0.65) was obtained between the levels of IgM-IKs measured by ELISA and by conventional hemagglutination assay, IgG-IKs and hemagglutination titers did not correlate (r = -0.15).
D000469:Alkaline Phosphatase; D000818:Animals; D000906:Antibodies / Q000032:analysis; D001323:Autoantibodies / Q000032:analysis* / Q000378:metabolism; D003176:Complement C3 / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis; D011817:Rabbits
D000906:Antibodies; D001323:Autoantibodies; D003176:Complement C3; D007106:Immune Sera; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D000469:Alkaline Phosphatase