A study of the conditions of the first phosphodiester bond formation by E. coli RNA polymerase.
D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology*; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D009943:Organophosphorus Compounds; D009994:Osmolar Concentration; D013379:Substrate Specificity
D009943:Organophosphorus Compounds; D008345:Manganese; D012321:DNA-Directed RNA Polymerases; D008274:Magnesium