A highly sensitive method for the demonstration of carcinoembryonic antigen in normal and neoplastic colonic tissue.
A highly sensitive method for the immuno-histochemical localisation of carcinoembryonic antigen (CEA) is described. This method is based on the binding of a peroxidase-antiperoxidase complex (PAP) to anti-CEA antibodies by means of an anti-gamma-globulin which reacts with both the anti-CEA and the antiperoxidase antibodies. Using the technique described here, CEA was localised in conventionally processed normal and cancerous colonic tissue. In normal as well as in neoplastic tissues, a CEA-specific staining of cell membranes and cytoplasm was demonstrated. In frozen sections of normal colonic tissue CEA was found even at high dilutions of the first antibody; this indicates the high sensitivity of the method. The applicability of the method to conventionally processed and thereby well preserved tissue specimens opens the possibility to identify CEA by light microscopy even at very low concentrations.
D000818:Animals; D002272:Carcinoembryonic Antigen / Q000276:immunology; D003110:Colonic Neoplasms / Q000276:immunology* / Q000473:pathology; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology; D007124:Immunoenzyme Techniques; D007163:Immunosorbent Techniques; D007413:Intestinal Mucosa / Q000473:pathology; D011817:Rabbits; D012995:Solubility; D013552:Swine
D002272:Carcinoembryonic Antigen; D007106:Immune Sera