A rapid technique for producing silver-stained nucleolus organizer regions and trypsin-giemsa bands on human chromosomes.
A simple and rapid technique is described whereby the nucleolus organizer regions (NORs) of human chromosomes can be differentially stained with silver. This staining is followed by trypsin-Giemsa banding on the same metaphase chromosomes. The metaphases simultaneously exhibit silver-stained NORs and G bands, allowing for the unequivocal identification of all chromosomes and greatly facilitating studies involving the NOR-bearing acrocentrics.
D001399:Azure Stains; D002466:Cell Nucleolus; D002877:Chromosomes, Human; D003584:Cytological Techniques; D005796:Genes; D005821:Genetic Techniques; D006801:Humans; D012834:Silver
D001399:Azure Stains; D012834:Silver