New immunofluorescent antibody test for diagnosis of candidiasis using macrophages-engulfed antigens as substrate.
A new method for detection of antibodies to polysaccharidic antigens of Candida albicans is described. The method is based on the selective uptake of carbohydrate-rich antigens by mouse peritoneal macrophages, the antibodies being revealed by indirect immunofluorescence after incubation of fixed, antigen-containing macrophages with sera (MU/IFA test). This test shows greater sensitivity than counterimmunoelectrophoresis individuals. The combination of CIE using cytoplasmic antigens and MU/IFA using surface antigens and MU/IFA using surface antigens is useful in a considerable majority of cases for diagnosis of clinical infection.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D000946:Antigens, Fungal; D002177:Candidiasis / Q000175:diagnosis*; D003378:Counterimmunoelectrophoresis; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007074:Immunoglobulin G; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D008811:Mice, Inbred DBA; D011817:Rabbits
D000888:Antibodies, Anti-Idiotypic; D000946:Antigens, Fungal; D007074:Immunoglobulin G