title: Transfection and transformation of Agrobacterium tumefaciens.
abstract: The freeze thaw transfection procedure of Dityatkin et al. (1972) was adapted for the transfection and transformation of A. tumefaciens. Transfection of the strains B6S3 and B6-6 with DNA of the temperate phage PS8cc186 yielded a maximum frequency of 2 10(-7) transfectants per total recipient population. In transformation of the strain GV3100 with the P type plasmid RP4 a maximum frequency of 3.5 10(-7) transformants per total recipient population was obtained. Agrobacterium Ti-plasmids were introduced in the strain GV3100 with a maximal efficiency of 4.5 10(-8). These experiments provide further evidence that the Ti-plasmid is responsible for the oncogenic properties of A tumefaciens and for its capacity to induce "opine" synthesis in Crown-gall plant cells.
mesh_terms: D001431:Bacteriological Techniques; D001435:Bacteriophages / Q000235:genetics*; D003090:Coliphages / Q000235:genetics; D008854:Microscopy, Electron; D012231:Rhizobium / Q000235:genetics* / Q000648:ultrastructure; D014162:Transfection; D014169:Transformation, Bacterial