[The differentiation of clostridial microcultures on nuclepore-filters by fluorescent antibodies as a rapid screening test for spores. II. Application of fluorescent antibodies (author's transl)].
The study deals with the question of whether a combination of microcolony-cultivation on polycarbonate-membrane-filters with the fluorescent-antibody-method will lead to a rapid-diagnosis method for clostridial-spores. Formaldehyd-inactivated vegetative cells of eight different clostridial-strains (Table 1) were used for the immunisation of rabbits according to the method published by Böhm and Strauch (5). The results of the immunisation procedure, developed for aerobic-sporeformers, showed, that satisfactory results could not be obtained in all cases (Table 2). The eight conjugates were tested against microcolonies of 32 strains of different clostridial-species on membrane-filters, produced by the technique described in the first communication. The results showed (Table 4) that differentiation was always possible when homologous antisera were used. In most cases, in order to differentiate between the various species, conjugates of several serotypes had to be employed together. However due to their uniform antigenic nature, Cl. feseri and Cl. tetani could both be identified by using one strain for each to immunize. With clostridial-spores the species could be identified within a period of 6 to 12 hours by using the membrane-filter-fluorescent-antibody technique.
D000907:Antibodies, Bacterial; D003013:Clostridium / Q000302:isolation & purification*; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D013171:Spores, Bacterial / Q000302:isolation & purification
D000907:Antibodies, Bacterial; D007106:Immune Sera
Die fluoreszenzserologische Differenzierung von Clostridienmikrokulturen auf Nuclepore-Membranfiltern als indirekter Sporenschnellnachweis II. Der fluoreszenzserologische Nachweis.