[Specific humoral immunity in the circulation of dogs with allotransplanted lungs].
A method appropriate for identification and quantification of circulating antibodies reacting with donorlungantigens is presented in allogenous lungtransplantation. Following incubation of native tissue-slides taken from the second removed but not grafted donor lung with the recipient's serum, the specific humoral antibodies are detected by indirect immunfluorescence. Quantifying evaluation is performed by serum-dilutions. The preexisting specific antibody-titer found in DLA-sensitized animals shows a significant correlation with the duration of graft function when compared with lymphocytotoxic antibody-titer. In unsensitized dogs a regular appearance of circulating specific antibodies can be seen beginning from the fourth or fifth postoperative day. The increasing antibody-titer during further course is a feature of a humoral graft rejection represented in the periphereal blood-circulation.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D000918:Antibody Specificity; D004285:Dogs; D006789:Host vs Graft Reaction; D007109:Immunity; D008168:Lung / Q000276:immunology; D016040:Lung Transplantation; D014181:Transplantation Immunology; D014184:Transplantation, Homologous
D000906:Antibodies
Spezifische humorale Immunität in der Zirkulation von lungenallotransplantierten Hunden.