[Heliomycin suppression of RNA synthesis in a cell-free system].
Heliomycin inhibited in vitro the RNA-polymerase reaction catalyzed by the preparation of DNA-dependent RNA-polymerase from E. coli. The blocking effect increased with a rise in the antibiotic concentration. The inhibitory effect of heliomycin decreased, when the amount of RNA-polymerase in the system increased. Yet, it did not depend on the content of DNA and the nature of the DNA preparation. Preincubation of RNA-polymerase with DNA resulting in formation of the enzyme-matrix complex did not prevent blocking RNA synthesis by heliomycin. Suppression of the RNA-polymerase reaction did not depend on the time of the antibiotic addition to the polymerizing system. Heliomycin had a significant activity not only with respect to the bacterial RNA-polymerase, but also in the system containing the enzyme isolated from the cells of Crithidia oncopelti.
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002474:Cell-Free System / Q000187:drug effects; D003090:Coliphages; D004269:DNA, Bacterial / Q000494:pharmacology; D012321:DNA-Directed RNA Polymerases / Q000037:antagonists & inhibitors; D004926:Escherichia coli / Q000201:enzymology; D056890:Eukaryota / Q000201:enzymology; D007783:Lactones / Q000494:pharmacology; D011083:Polycyclic Compounds; D012329:RNA, Bacterial / Q000037:antagonists & inhibitors*; D013211:Staphylococcus aureus / Q000187:drug effects
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D007783:Lactones; D011083:Polycyclic Compounds; D012329:RNA, Bacterial; C007912:heliomycin; D012321:DNA-Directed RNA Polymerases
Podavlenie geliomitsinom sinteza RNK v beskletochnoĭ sisteme.