Homogeneous enzyme immunoassay of serum digoxin with use of a bichromatic analyzer.
We applied the Enzyme Multiplied Immunoassay Technique (EMIT; Syva Corp., Palo Alto, CA) for determination of serum digoxin to the ABA-100 bichromatic analyzer. Assay conditions were almost exactly as prescribed for the manual procedure, but the ABA-100 offers high automation, smaller reagent volumes, and shorter reaction time. Precision studies gave CV's of less than 10%. Sixty patients' samples, analyzed for digoxin by radioimmunoassay and this enzyme immunoassay, gave a correlation (r) of 0.941. Results obtained with the ABA-100 were apparently slightly higher. One kit provides reagents for 250 assays, as compared to 70 assays with the manual procedure. In an emergency situation a result will be available about 60 min after the patient's sample is received; one operator can analyze about 120 samples in 8 h.
D004077:Digoxin / Q000097:blood*; D006461:Hemolysis; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood; D007124:Immunoenzyme Techniques; D007565:Jaundice / Q000097:blood; D011863:Radioimmunoassay / Q000379:methods; D011933:Reagent Kits, Diagnostic
D011933:Reagent Kits, Diagnostic; D004077:Digoxin