Insertion of the Tn3 transposon into the genome of the single-stranded DNA phage M13.
The transposable genetic element Tn3, which carries an ampicillin (Ap) resistance determinant, has been translocated from a ColE1-Apr plasmid, RSF2124, to the genome of the filamentous single-stranded DNA phage M13. The site orientation of the inserted element has been determined for one such phage, M13::Tn3-15. The insertion is within the intergenic space separating genes 2 and 4 and containing both the viral strand and complementary strand origins. The lengths of both the filamentous phage and the duplex replicative form (RF) DNA are 1.7--1.8 times those of M13 phage and replicative form DNA. Both plaque formation and transduction of sensitive cells to ampicillin resistance by M13::Tn3-15 are sensitive to purified antibodies to the M13 major coat protein.
D000667:Ampicillin / Q000494:pharmacology; D003090:Coliphages / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D005814:Genes, Viral; D008854:Microscopy, Electron; D010403:Penicillin Resistance
D004274:DNA, Recombinant; D004279:DNA, Viral; D000667:Ampicillin; D004262:DNA Restriction Enzymes