Antigen-antibody complexes suppress antibody production by mouse plasmacytoma cells in vitro.
Preformed complexes of 2,4-dinitrophenylated keyhole limpet hemocyanin (DNP-KLH) and anti-DNP or anti-KLH antibodies selectively, suppressed the production of anti-DNP IgA by the plasmacytoma cell line, MOPC 315, in vitro. Compexes made with DNP8.5KLH (8.5 moles DNP/10(5) daltons carrier) inhibited the secretion of IgA but not its synthesis, whereas complexes prepared with the more highly substituted DNP20KLH inhibited synthesis as well. Moreover, complexes of DNP-KLH and anti-DNP F(ab')2 fragments were as suppressive as those made with IgG antibodies, suggesting that the mode of presentation of antigenic determinants and not surface Fe receptors may play a major role in this phenomenon.
D000818:Animals; D000917:Antibody Formation; D000936:Antigen-Antibody Complex; D000941:Antigens; D002460:Cell Line; D006433:Hemocyanins / Q000276:immunology; D006462:Hemolytic Plaque Technique; D007141:Immunoglobulin Fc Fragments; D051379:Mice; D008810:Mice, Inbred C57BL; D009374:Neoplasms, Experimental / Q000276:immunology; D010954:Plasmacytoma / Q000276:immunology*
D000936:Antigen-Antibody Complex; D000941:Antigens; D007141:Immunoglobulin Fc Fragments; D006433:Hemocyanins