Relative sensitivities of forward and reverse mutation assays in Salmonella typhimurium.
Forward mutation to 8-azaguanine resistance and reverse mutation to histidine prototrophy were measured in Salmonella typhimurium after treatment with 16 mutagens of both base-substitution and frameshift classes. The two approaches were found to be equisensitive for all 16 mutagens--i.e., induction of significant mutation occurred at similar concentrations in the forward mutation assay and in the most sensitive of the five Ames tester strains.
D000818:Animals; D001375:Azaguanine / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D004352:Drug Resistance, Microbial; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism; D009153:Mutagens; D009154:Mutation; D051381:Rats; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics*
D009153:Mutagens; D001375:Azaguanine