title: Lysyl-derived aldehydes in outer membrane proteins of Escherichia coli.
abstract: The major outer membrane proteins from Escherichia coli K-12 are modified to contain alpha-aminoadipic acid delta-semialdehyde (allysine). The allysine was found to be derived from lysine and it was identified by derivatizing it to chloronorleucine by reduction, alpha-aminoadipic acid by oxidation, and to alpha,epsilon-diaminopimelic acid by reacting it with CN- and NH3. The alpha-aminoadipic acid was identified by mass spectrometry. Two major outer membrane proteins were found to possess allysine, a modified lysine characteristically found to connective tissue.
mesh_terms: D015074:2-Aminoadipic Acid / Q000031:analogs & derivatives / Q000032:analysis*; D000447:Aldehydes / Q000032:analysis; D000600:Amino Acids, Dicarboxylic / Q000032:analysis*; D002855:Chromatography, Thin Layer; D004926:Escherichia coli / Q000378:metabolism*; D013058:Mass Spectrometry; D008565:Membrane Proteins
chemical_list: D000447:Aldehydes; D000600:Amino Acids, Dicarboxylic; D008565:Membrane Proteins; D015074:2-Aminoadipic Acid; C000061:allysine