Formation of HfrH-type donor cells as a result of integrative suppression by R-F recombinant plasmids.
Three recombinant plasmids, resulting from recombination between an R plasmid of the FI incompatibility group and the F of HfrH, were introduced in a temperature-sensitive dnaA mutant to isolate Hfr-type-donors. All of the temperature-insensitive clones isolated from two of the three recombinant plasmids had the same origin and transfer pattern as the parental HfrH strain.
D002876:Chromosomes, Bacterial; D003227:Conjugation, Genetic; D004926:Escherichia coli / Q000235:genetics* / Q000648:ultrastructure; D005144:F Factor; D011815:R Factors; D011995:Recombination, Genetic; D013696:Temperature