Intracellular pancreatic B cell serotonin and the dynamics of insulin release.
The role of intracellular pancreatic B cell serotonin in the dynamics of insulin release was investigated in an in situ perfused rat pancreas preparation. Animals were pretreated with 5-hydroxytryptophan (5-HTP) to increase the intracellular levels of serotonin (5-HT) as shown by fluorescence histochemistry. Despite a clear induction of intracellular 5-HT fluorescence in pancreatic islets neither the pattern nor the total amount of insulin released were significantly modified after perfusion with either glucose or tolbutamide. However, the L-amino acid decarboxylase inhibitor, RO 4--4602, significantly decreased both phases of glucose-mediated insulin release in normal animals as well as in those receiving 5-HTP.
D006916:5-Hydroxytryptophan / Q000494:pharmacology; D000818:Animals; D005453:Fluorescence; D005947:Glucose / Q000494:pharmacology; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D009526:Nialamide / Q000494:pharmacology; D051381:Rats; D012701:Serotonin / Q000502:physiology*; D014044:Tolbutamide / Q000494:pharmacology
D007328:Insulin; D012701:Serotonin; D014044:Tolbutamide; D006916:5-Hydroxytryptophan; D005947:Glucose; D009526:Nialamide