Calcium and pancreatic beta-cell function: glucose stimulation uptake of lanthanum-displaceable 45-Ca from low or normal calcium-containing media.
The uptake of 45Ca was studied in beta-cell-rich pancreatic islets microdissected from ob/ob-mice. Glucose stimulated 45Ca incorporation in a lanthanum-displaceable pool remaining after correction for the extracellular space occupied by sucrose. It is suggested that the glucose effect on the lanthanum-displaceable calcium is the cause rather than the result of the secretion of insulin since it could be demonstrated also in the presence of only trace amounts of extracellular calcium.
D000818:Animals; D002118:Calcium / Q000378:metabolism*; D003470:Culture Media; D005947:Glucose / Q000494:pharmacology*; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D007811:Lanthanum / Q000378:metabolism; D008297:Male; D051379:Mice; D008820:Mice, Obese / Q000378:metabolism
D003470:Culture Media; D007328:Insulin; D007811:Lanthanum; D005947:Glucose; D002118:Calcium