[Possibilities of viral hepatitis diagnosis by immunofluorescent examination of patient blood smears].
The authors demonstrate possibilities of viral hepatitis diagnosis by means of fluorescent antisera labeled phiiTts. The antisera were obtained from the blood of convalescents after infectious hepatitis (with an uncomplicated course) on the 25th--30th day of the disease, and also from the placental gamma-globulin of persons who had had viral hepatitis (in the anamnesis). Both diagnostic preparations proved to be highly specific, although the first of them was somewhat more sensitive. Blood smears were stained with fluorescent antisera. The fluoresence was revealed in the cytoplasm of the white blood formed elements--lymphocytes and neutrophils; the nucleus was dark. Materials of this investigation permit experimental substantiation of a possibility of prolonged antigenemia in the blood leukocytes of patients suffering from viral hepatitis.
D000956:Antigens, Viral / Q000032:analysis; D003289:Convalescence; D003593:Cytoplasm / Q000276:immunology; D003937:Diagnosis, Differential; D005455:Fluorescent Antibody Technique; D006525:Hepatitis, Viral, Human / Q000175:diagnosis*; D006652:Histological Techniques; D006801:Humans; D008214:Lymphocytes / Q000276:immunology; D009504:Neutrophils / Q000276:immunology
D000956:Antigens, Viral
O vozmozhnosti diagnostiki virusnogo gepatita pri obsledovanii mazkov krovi bol'nykh s pomoshch'iu metoda immunofliuorestsentsii.