A sensitive haemolytic assay of mouse complement.
Under appropriate conditions the haemolytic activity of mouse complement can be measured in a 51Cr release assay. Modifications that increase the sensitivity are the following: (a) mouse 7 S anti-SRBC antibodies as amboceptor; (b) low amount of target cells; (c) ionic strength of 0.13 M NaCl in the test medium; (d) incubation temperature of 30 degrees C; (e) incubation time of 90 min; (f) total reaction volume of 1 ml.
D000818:Animals; D003165:Complement System Proteins / Q000378:metabolism*; D006462:Hemolytic Plaque Technique; D008722:Methods; D051379:Mice; D009994:Osmolar Concentration; D013696:Temperature; D013997:Time Factors
D003165:Complement System Proteins