Combined Nomarski interference contrast and immunofluorescent study of neuropathological specimens: CSF sediments and paraffin embedded brain tissues.
The direct immunofluorescent technique may be easily improved by the use of the Nomarski optics. This contrast allows accurate identification of fluorescent CSF cells and structures in formalin fixed paraffin embedded brain tissues; in the latter, the combined optical procedure is fruitfull in order to avoid fluorescent artifacts misinterpretation. Furthermore, it is emphazised that the conditions in which routine neuropathological specimens are removed and stored usualy does permit the application of the immunofluorescent technique.
D001921:Brain / Q000473:pathology*; D002555:Cerebrospinal Fluid / Q000166:cytology*; D005455:Fluorescent Antibody Technique; D006801:Humans; D008857:Microscopy, Interference; D008858:Microscopy, Phase-Contrast; D009422:Nervous System Diseases / Q000134:cerebrospinal fluid / Q000473:pathology*