Analysis of heteroantisera to cells from human malignant effusions by immunofluorescence and protein A binding.
Using cultured cells derived from human malignant effusions, hetero-antisera were raised in rabbits. The antisera were sequentially absorbed on various human non-tumour cells, reactivity being monitored by immunofluorescence and 125I-labelled staphylococcal protein A assays. The absorbed antisera possessed common reactivity to all tumour cells assayed. This reactivity was not histogenically determined, and our data suggest that it was not directed to oncofoetal antigens.
D000818:Animals; D000910:Antibodies, Heterophile / Q000032:analysis*; D000912:Antibodies, Neoplasm / Q000032:analysis*; D000937:Antigen-Antibody Reactions; D000951:Antigens, Neoplasm; D001666:Binding Sites, Antibody; D002460:Cell Line; D005347:Fibroblasts / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006801:Humans; D008545:Melanoma / Q000276:immunology; D009369:Neoplasms / Q000276:immunology*; D011817:Rabbits; D013205:Staphylococcal Protein A / Q000032:analysis*
D000910:Antibodies, Heterophile; D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D013205:Staphylococcal Protein A