title: Determination of pools of tricarboxylic acid cycle and related acids in bacteria.
abstract: Methods for sampling, extracting, and quantitating the metabolic pools of organic acids from bacteria have been developed. The concentration of these metabolites was determined by a new gas chromatographic method that can quantitatively determine the levels of lactate, pyruvate, fumarate, succinate, malate, alpha-ketoglutarate, and citrate. Values obtained were confirmed by fluorimetric analyses of five of the individual acids. In Escherichia coli, pools range from about 1 to 5 mumol/g of dry weight, with a variation in replicate samples of 5 to 15%. Under similar conditions, these pools in Bacillus licheniformis are in the same range, although the pyruvic acid pool is significantly larger.
mesh_terms: D001407:Bacillus / Q000032:analysis* / Q000378:metabolism; D002264:Carboxylic Acids / Q000032:analysis*; D002849:Chromatography, Gas; D002951:Citrates / Q000032:analysis; D002952:Citric Acid Cycle; D004926:Escherichia coli / Q000032:analysis* / Q000378:metabolism; D005470:Fluorometry; D005650:Fumarates / Q000032:analysis; D005947:Glucose / Q000378:metabolism; D005971:Glutamates / Q000378:metabolism; D007656:Ketoglutaric Acids / Q000032:analysis; D007773:Lactates / Q000032:analysis; D008293:Malates / Q000032:analysis; D011773:Pyruvates / Q000032:analysis; D013386:Succinates / Q000032:analysis
chemical_list: D002264:Carboxylic Acids; D002951:Citrates; D005650:Fumarates; D005971:Glutamates; D007656:Ketoglutaric Acids; D007773:Lactates; D008293:Malates; D011773:Pyruvates; D013386:Succinates; D005947:Glucose