title: [Cloning chloroplast DNA in escherichia coli. I. Construction and selection of recombinant plasmids containing fragments of pea chloroplast DNA].
abstract: Fragments produced by digestion of Pisum sativum chloroplast DNA with EcoRI were examined by agarose gel electrophoresis. These EcoRI-fragments were joined in vitro to Apr-ColE1 RSF2124 plasmid and cloned in Escherichia coli. Methods of molecular cloning of plasmid chimeras by success gradient centrifugation and repeated transformation and selection of recombinant plasmids using mytomicin C were used for cloning hybrid plasmids with various EcoRI fragments of pea chloroplast DNA has been obtained.
mesh_terms: D002736:Chloroplasts / Q000032:analysis*; D002999:Clone Cells; D004261:DNA Replication; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D010944:Plants / Q000235:genetics; D010957:Plasmids; D011995:Recombination, Genetic; D014162:Transfection
vernacular_title: Klonirovanie DNK khloroplastov v Escherichia coli. Soobshchenie I. Konstruktsiia i selektsiia rekombinantnykh plazmid, soderzhashchikh fragmenty DNK khloroplastov gorokha.