Monolayer cultures for immunofluorescent staining from tumor primary explants.
Epithelial cells growing around primary explants of carcinomas in plastic ware are well-suited for modern incident light immunofluorescence microscopy. Epithelial outgrowths in growth culture medium are flushed with phosphate-buffered saline (PBS) and absolute ethanol and snap-frozen in liquid nitrogen-isopentane. The walls of the plastic containers bearing the dried monolayer outgrowths are cut out to form microscopic slides. Immunofluorescence tests are made on circular areas demarcated on the monolayers by using small metal cylinders to mask against a water-repellent plastic spray. More than 20 immunofluorescence tests can be performed on a culture 40 X 50 mm.
D000951:Antigens, Neoplasm; D001943:Breast Neoplasms / Q000276:immunology; D046508:Culture Techniques / Q000379:methods*; D004848:Epithelium / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D009374:Neoplasms, Experimental / Q000276:immunology*; D011471:Prostatic Neoplasms / Q000276:immunology
D000951:Antigens, Neoplasm