Reversal of diabetes in rats using fetal pancreases stored at -196 C.
Fetal rat pancreases frozen to and stored at -196 C were used for transplantation into streptozotocin-induced diabetic syngeneic adult recipients. Transplantation was carried out either directly after thawing from -196 C, or after a 21-day growth period in a syngeneic, normoglycemic adult carrier. All transplants were placed under the kidney capsule. A single, frozen fetal rudiment was sufficient to restore blood glucose, urine volume, and urine glucose to normal, provided it had first been grown for 21 days in a normal carrier. It vitro perfusion studies showed that fetal pancreases stored at -196 C were equivalent to fresh rudiments in their responses to a glucose stimulus.
D000818:Animals; D001786:Blood Glucose; D001835:Body Weight; D003921:Diabetes Mellitus, Experimental / Q000276:immunology* / Q000378:metabolism; D005260:Female; D005333:Fetus / Q000276:immunology*; D005615:Freezing; D006029:Glycosuria; D006943:Hyperglycemia / Q000150:complications; D007328:Insulin / Q000096:biosynthesis; D009926:Organ Preservation; D016035:Pancreas Transplantation; D011247:Pregnancy; D051381:Rats; D011917:Rats, Inbred Lew; D014021:Tissue Preservation; D014184:Transplantation, Homologous
D001786:Blood Glucose; D007328:Insulin