Helper factor(s) for growth of adeno-associated virus in cells transformed by adenovirus 12.
Evidence is presented that a helper factor(s) for growth of adeno-associated virus (AAV) is present in cells transformed by adenovirus type 12 (Ad12). The growth of AAV was observed in heterokaryons formed by fusion of human KB and Ad12-transformed rodent cells by using ultraviolet-inactivated Sendai virus without coinfection of cells with adenovirus. The presence of the helper factor(s) for AAV growth in rat cells transformed by the EcoRI-C fragment or the HindIII-G fragment of Ad12DNA suggests that the helper factor(s) induced by infection with adenovirus is the Ad12-specific T antigen.
D000256:Adenoviridae / Q000502:physiology*; D000956:Antigens, Viral / Q000032:analysis*; D001345:Autoradiography; D002459:Cell Fusion; D002460:Cell Line; D002472:Cell Transformation, Viral; D004279:DNA, Viral / Q000096:biosynthesis; D005455:Fluorescent Antibody Technique; D012526:Satellite Viruses / Q000254:growth & development*; D013997:Time Factors
D000956:Antigens, Viral; D004279:DNA, Viral