Inhibition of prostaglandin-enhanced release of LH by antiserum to luteinizing hormone releasing hormone.
Prostaglandin E2 (PGE2), PGF2alpha, PGF2beta was infused into a lateral ventricle of the brain of adult male rats, after pretreatment with normal rabbit serum (NRS) or anti-LH-RH serum, and the concentration of LH in arterial plasma was determined. I.v. administration of anti-LH-RH serum 2.5 min prior to the infusion of 2 microgram or 20 microgram of PGE2 significantly inhibited the PGE2-induced rise of plasma LH. Intraventricular infusion of 20 microgram of PGF2alpha or PGF2beta into NRS-pretreated animals caused a marked increase in the plasma LH concentration; whereas, prior i.v. administration of anti-LH-RH serum blocked the PG-induced rise in plasma LH levels. It is concluded that PGE2, PGF2alpha, and PGF2beta stimulate the release of LH primarily by enhancing the release of LH-RH.
D000818:Animals; D000937:Antigen-Antibody Reactions; D007987:Gonadotropin-Releasing Hormone / Q000276:immunology / Q000502:physiology*; D007106:Immune Sera; D007986:Luteinizing Hormone / Q000378:metabolism*; D008297:Male; D011453:Prostaglandins / Q000494:pharmacology*; D011458:Prostaglandins E / Q000494:pharmacology; D011460:Prostaglandins F / Q000494:pharmacology; D051381:Rats
D007106:Immune Sera; D011453:Prostaglandins; D011458:Prostaglandins E; D011460:Prostaglandins F; D007987:Gonadotropin-Releasing Hormone; D007986:Luteinizing Hormone