Antigen-specific helper factor in man.
Supernates of tetanus toxoid (TT) antigen-stimulated human T cells were studied for the presence of an antigen-specific T-cell helper factor (ASF). Supernates were circulated over an immunosorbent column consisting of insolubilized TT antigen. The material which bound to the column was eluted with 3 M NaCNS and was shown to contain a factor which in the presence of TT-induced specific IgG anti-TT antibody synthesis in autologous B cells without causing readily detectable proliferation. ASF activity was partially inhibited by antisera directed against the B-cell alloantigens of the ASF donor. Immunosorbent columns containing such antisera removed ASF activity. Immunosorbent columns containing antisera to human immunoglobulin heavy chain determinants did not remove ASF activity; whereas immunosorbent columns containing rabbit idiotypic antiserum directed against anti-TT antibodies completely removed ASF activity. ASF was destroyed by treatment with proteolytic enzymes; its molecular weight was estimated by Sephadex G-100 gel column chromatography to be between 25,000 and 75,000 daltons.
D000917:Antibody Formation; D001402:B-Lymphocytes / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis; D007130:Immunoglobulin Idiotypes; D007163:Immunosorbent Techniques; D008211:Lymphocyte Cooperation; D008222:Lymphokines / Q000302:isolation & purification; D008970:Molecular Weight; D013601:T-Lymphocytes / Q000276:immunology*; D013743:Tetanus Antitoxin; D013745:Tetanus Toxoid
D007074:Immunoglobulin G; D007130:Immunoglobulin Idiotypes; D008222:Lymphokines; D013743:Tetanus Antitoxin; D013745:Tetanus Toxoid