Simultaneous determination of insulin secretion and glucose oxidation rates during incubation of isolated rat islets of Langerhans.
Insulin secretion being related to glucose metabolism of the B cell, it seemed of interest to develop a suitable system to measure these two parameters on the same islet pool, in order to overcome the great individual differences observed when these are measured in separate experiments. For this reason, we used an incubation system allowing the measurement of insulin secretion concomitantly with the determination of the corresponding oxidation rate. The results obtained agree well with those described by several authors using separate experimental procedures. Thus, our experimental design appears to be a reliable method for simultaneous determination of insulin secretion and glucose oxidation rates.
D000818:Animals; D005947:Glucose / Q000378:metabolism*; D006019:Glycolysis; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000378:metabolism*; D007700:Kinetics; D008297:Male; D051381:Rats
D007328:Insulin; D005947:Glucose