Inhibition enzyme immunoassay, application to human apolipoprotein B.
Inhibition enzyme immunoassay was applied to human apolipoprotein B (apo-B) from plasma. The technical conditions of the assay were determined. The detection limits of the assay were 200 ng to 10 microgram/ml. Correlation coefficients obtained between enzymoassay and rocket immunoelectrophoresis on one hand and radial immunodiffusion on the other were respectively 0.84 and 0.80. The inhibition enzymoassay provides a specific and highly sensitive method for the quantitation of apo-B.
D000906:Antibodies; D000941:Antigens; D001053:Apolipoproteins / Q000097:blood*; D006801:Humans; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D008074:Lipoproteins
D000906:Antibodies; D000941:Antigens; D001053:Apolipoproteins; D007106:Immune Sera; D008074:Lipoproteins