Effects of high temperature on Escherichia coli F pili.
The effects of high temperatures (46 to 50 degrees C) on the production of F pili by Escherichia coli were studied by electron microscopy. Attached F pili rapidly disappeared at 48 and 50 degrees C but not at 46 degrees C. Free pili were not denatured at these temperatures. The pili that disappeared from the cells at 50 degrees C did not appear as free pili in the culture supernatant fluid, indicating that the pili had retracted to the cell surface or into the cell. The adsorption of either R17 phage or F pili antibody to the sides of pili prevented retraction. The disappearance of pili was accompanied by a loss in the ability to adsorb R17 phage but not M13 phage, suggesting that the tip of a pilus remains exposed after retraction.
D000907:Antibodies, Bacterial; D002462:Cell Membrane / Q000648:ultrastructure; D003090:Coliphages; D003227:Conjugation, Genetic; D004926:Escherichia coli / Q000648:ultrastructure*; D005144:F Factor; D006358:Hot Temperature; D008854:Microscopy, Electron
D000907:Antibodies, Bacterial