Cathodoluminescence applied to immunofluorenscence: present state and improved technical prospects by prism spectrometer light selection.
For studies on cathodoluminescence, we equipped a scanning electron microscope with a prism spectrometer and sensitive photomultiplier. The apparatus is described and our initial results are presented on the analyse of cathodoluminescence. The material observed promarily involved studies of immunofluorescent specimens. Humal lymphocytes were labelled with a fluorescent antibody and cryosections of rat kidney with Masugi nephritis were labelled with a fluorescent specific antibody. Our apparatus permitted monochromatic imaging of cathodoluminescence emissions and resulted in much improved micrographs. Some possible improvements of the technique are discussed.
D000818:Animals; D000906:Antibodies / Q000032:analysis; D001610:Beta Particles; D005455:Fluorescent Antibody Technique; D006801:Humans; D007668:Kidney / Q000276:immunology; D008163:Luminescent Measurements; D008214:Lymphocytes / Q000276:immunology; D008855:Microscopy, Electron, Scanning; D051381:Rats; D013057:Spectrum Analysis
D000906:Antibodies