Quantitation of chlordiazepoxide and its metabolites in biological fluids by thin-layer chromatography.
Chlordiazepoxide and its 4 major metabolites were assayed after separation by thin-layer chromatography following extraction from biological fluids. The compounds become intensely fluorescent in the presence of red, fuming nitric acid. The resulting compounds are quantitated with a spectrodensitometer with a fluorescent attachment. The sensitivity varies between 0.05 and 0.1 microgram. The coefficient of variation is 1.4% for assays in urine and 6.4% in serum.
D001570:Benzodiazepinones / Q000097:blood / Q000652:urine; D002707:Chlordiazepoxide / Q000031:analogs & derivatives / Q000032:analysis* / Q000097:blood / Q000652:urine; D002855:Chromatography, Thin Layer; D006801:Humans; D003708:Nordazepam / Q000097:blood / Q000652:urine; D010076:Oxazepam / Q000097:blood / Q000652:urine; D013050:Spectrometry, Fluorescence
D001570:Benzodiazepinones; D003708:Nordazepam; D010076:Oxazepam; D002707:Chlordiazepoxide