"Panning" for lymphocytes: a method for cell selection.
We have developed a simple method for the fractionation of T and B lymphocytes. Plastic dishes coated with antibodies specific for mouse Ig selectively bind splenic B lymphocytes. The adherent cells are easily removed by gentle pipetting; both adherent and nonadherent populations retain immunologic function. In a typical experiment, when 3 X 10(7) splenic lymphocytes were added to a 100 X 15 mm plastic dish coated with microgram quantities of anti-Ig, 98 % of the nonadherent cells were Ig negative and 97% of the adherent cells were Ig positive. The method is sufficiently sensitive to allow detection and separation of cell types comprising as little as 2% of the total population and can be modified to allow the selection of cells by a double-antibody procedure. We believe that the plastic dish method will be generally useful for fractionating cells on the basis of their cell surface antigens.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D002469:Cell Separation / Q000379:methods*; D007140:Immunoglobulin Fab Fragments; D007158:Immunologic Techniques; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008934:Mitogens; D011137:Polystyrenes; D011947:Receptors, Antigen, B-Cell; D013154:Spleen / Q000166:cytology
D000888:Antibodies, Anti-Idiotypic; D007140:Immunoglobulin Fab Fragments; D008934:Mitogens; D011137:Polystyrenes; D011947:Receptors, Antigen, B-Cell