Immunohistochemical localisation of ecdysteroids in the follicular epithelium of locust oocytes.
Frozen sections of growing terminal follicles of the locust ovary were incubated with an ecdysteroid-specific rabbit antibody and the bound antibody visualised by the use of FITC-labelled goat-anti-rabbit antiserum. A bright fluorescence was seen in the cytoplasm of the follicle cells in terminal follicles with a length between 4.0 and 6.0 mm with a maximum intensity at 5.5 mm, indicating the presence of ecdysteroids in these cells in this particular developmental stage.
D000818:Animals; D004441:Ecdysterone / Q000032:analysis* / Q000276:immunology; D005260:Female; D005455:Fluorescent Antibody Technique; D006110:Grasshoppers; D006651:Histocytochemistry; D009865:Oocytes / Q000032:analysis*; D010063:Ovum / Q000032:analysis*
D004441:Ecdysterone