Synthesis of lens crystallins in Xenopus oocytes as determined by quantitative immunoprecipitation.
Total poly(A)-containing calf lens mRNA was microinjected into Xenopus oocytes and synthesis of alpha, beta, and gamma-crystallins was demonstrated. By a method of quantitative immunoprecipitation the rate of translation of purified 14S alphaA2-crystallin mRNA was compared with translation of 9-S rabbit globin mRNA. Maximal response of oocytes was obtained with virtually the same molar amounts of mRNA, taking into account the larger size of the alphaA2-crystallin mRNA. Kinetics of translation were also very similar and both mRNAs were translated with similar rate and efficiency for at least two days. It was estimated that 20-30 polypeptide chains per hour per mRNA molecule were synthesized.
D000818:Animals; D002417:Cattle; D011232:Chemical Precipitation; D003459:Crystallins / Q000032:analysis / Q000096:biosynthesis* / Q000276:immunology; D004305:Dose-Response Relationship, Drug; D005260:Female; D007158:Immunologic Techniques; D007700:Kinetics; D009865:Oocytes / Q000378:metabolism*; D010063:Ovum / Q000378:metabolism*; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000008:administration & dosage / Q000378:metabolism*; D014981:Xenopus
D003459:Crystallins; D012333:RNA, Messenger