Inhibin from cultures of rat seminiferous tubules.
Medium from cultures of mature rat seminiferous tubules contained a substance which suppressed, in a dose-related manner, the luteinizing hormone releasing hormone (LH-RH)-stimulated secretion of FSH by cultured rat pituitary cells. The secretion of LH was suppressed to a lesser extent and the basal secretion of both LH and FSH was inconsistently affected. Gel filtration on Sephadex G-100 did not fractionate the activity. The active material did not inhibit the secretion of TSH or destroy LH-RH and the activity was not due to testosterone or oestradiol in the medium. Control media from liver cultures were inactive. It is concluded that inhibin is present in media from cultures of rat seminiferous tubules.
D000818:Animals; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005640:Follicle Stimulating Hormone / Q000378:metabolism; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology; D007986:Luteinizing Hormone / Q000378:metabolism; D008297:Male; D009924:Organ Culture Techniques; D010902:Pituitary Gland / Q000166:cytology / Q000378:metabolism; D010905:Pituitary Hormone Release Inhibiting Hormones / Q000096:biosynthesis* / Q000494:pharmacology; D051381:Rats; D012636:Secretory Rate / Q000187:drug effects; D012671:Seminiferous Tubules / Q000378:metabolism*; D013735:Testicular Hormones / Q000096:biosynthesis* / Q000494:pharmacology; D013737:Testis / Q000378:metabolism*; D013972:Thyrotropin / Q000378:metabolism
D010905:Pituitary Hormone Release Inhibiting Hormones; D013735:Testicular Hormones; D007987:Gonadotropin-Releasing Hormone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D013972:Thyrotropin