title: A fixation-decalcification procedure for bone biopsies.
abstract: A procedure for the simultaneous fixation and decalcification of bone biopsies is described. Mild acidic fixatives (such as Bouin's fluid) are effective decalcifying agents when made calcium-free by a continuous circulation through a column of anion-binding ion exchange resin. The procedure has been found relatively fast, safe, economic and to provide excellent histology of biopsies containing cancellous bone.
mesh_terms: D001706:Biopsy; D001847:Bone Diseases / Q000473:pathology*; D001842:Bone and Bones / Q000473:pathology*; D003648:Decalcification Technique; D005404:Fixatives; D006652:Histological Techniques
chemical_list: D005404:Fixatives