Construction of a urocanic acid-producing strain of Serratia marcescens by transduction.
In Serratia marcescens, the mutation responsible for triazolealanine (TRA) resistance was transferred from a TRA-resistant mutant to a urocanase-less mutant by PS20-mediated transduction. The two crosses were performed using as donors two TRA-resistant mutants, whose phenotypes included increased levels of histidine-biosynthetic enzymes and feedback-insensitive phosphoribosyltransferase. In one cross, TRA-resistant transductants were urocanase-less mutants having only increased levels of the enzymes and barely detectable levels of urocanic acid. In the other cross, the transductants were urocanase-less mutants having both phenotypes of the donor, and most produced high concentrations (10.5 mg/ml) of urocanic acid.
D000409:Alanine / Q000031:analogs & derivatives / Q000494:pharmacology; D004352:Drug Resistance, Microbial; D004794:Enzyme Repression; D006638:Histidine Ammonia-Lyase / Q000096:biosynthesis; D007093:Imidazoles / Q000378:metabolism*; D009154:Mutation; D012706:Serratia marcescens / Q000378:metabolism*; D014161:Transduction, Genetic; D014560:Urocanic Acid / Q000378:metabolism*
D007093:Imidazoles; D006638:Histidine Ammonia-Lyase; D014560:Urocanic Acid; D000409:Alanine