title: Actin filaments in paramyxovirus-infected human fibroblasts studied by indirect immunofluorescence.
abstract: Fibroblasts growing on glass have microfilaments arranged in bundles. These can be demonstrated by indirect immunofluorescent technique using human antiactin serum or experimentally produced rabbit anti-actin serum. When monolayer cultures of epithelial cells and fibroblasts are infected with paramyxovirus, such as measles, mumps, Sendai and NDV, there is a striking decrease of the bundles. Rabies and adenoviruses do not seem to influence the staining of microfilaments. The microfilament decreasing effect in the cells correlates to the finding by SDS-polyacrylamide-gel-electrophoresis of actin within virions of the paramyxoviruses.
mesh_terms: D000199:Actins / Q000378:metabolism*; D000260:Adenoviruses, Human / Q000254:growth & development; D002460:Cell Line; D003593:Cytoplasm / Q000378:metabolism*; D003599:Cytoskeleton / Q000378:metabolism*; D005347:Fibroblasts; D005455:Fluorescent Antibody Technique; D006801:Humans; D007181:Inclusion Bodies, Viral; D010252:Paramyxoviridae / Q000254:growth & development*; D011820:Rabies virus / Q000254:growth & development; D014779:Virus Replication
chemical_list: D000199:Actins