title: Cloning and amplification of alpha and beta mouse globin gene sequences synthesised in vitro.
abstract: New chimeric Escherichia coli plasmids containing alpha or beta globin gene sequences of the mouse were constructed. Double-stranded DNA, synthesised in vitro in a 2-step reaction from mouse globin mRNA was inserted into E. coli plasmid pCR1, after tailing of the 2 DNAs with dG and dC respectively. Some of the mouse globin plasmids described contain at least 90% of the globin mRNA sequence and therefore contain the entire translated sequence of the globin genes. Some possible uses of these recombinant plasmids are described.
mesh_terms: D000818:Animals; D001483:Base Sequence; D004261:DNA Replication; D004269:DNA, Bacterial; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics; D005914:Globins / Q000235:genetics*; D051379:Mice / Q000235:genetics*; D010957:Plasmids; D014176:Protein Biosynthesis; D011498:Protein Precursors / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits
chemical_list: D004269:DNA, Bacterial; D004274:DNA, Recombinant; D011498:Protein Precursors; D012333:RNA, Messenger; D005914:Globins