Nuclei of cultured synovial fibroblasts studied by immunofluorescence and microdensitometry.
Two techniques which might be expected to detect alterations in DNA have been used in a comparison of cultured non-rheumatoid and rheumatoid synovial fibroblasts. Microdensitometry showed no alteration in staining affinity for methyl green when two pairs of cultures were compared. There was, however, a minor difference in the predominant staining pattern to a fluorescein conjugated anti-IgM serum when five rheumatoid cultures were compared with five non-rheumatoid cultures after exposure to a serum containing anti-nuclear antibodies.
D002467:Cell Nucleus / Q000032:analysis*; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis*; D003720:Densitometry; D005347:Fibroblasts / Q000032:analysis; D005455:Fluorescent Antibody Technique; D006801:Humans; D012216:Rheumatic Diseases / Q000378:metabolism*; D013583:Synovial Membrane / Q000032:analysis* / Q000166:cytology
D004247:DNA