Detection of antibodies to legionnaires disease organism by microagglutination and micro-enzyme-linked immunosorbent assay tests.
Microagglutination and micro-enzyme-linked immunosorbent assay (ELISA) tests with easily prepared, safe, heat-killed antigens for detecting antibodies to the legionnaires disease organism have been developed. A safranin-stained whole-cell antigen is used in the microagglutination test, and a simply prepared soluble antigen is used in the micro-ELISA tests. The microagglutination test detected elevated titers in 97.2% of the sera from patients with legionnaires disease. Three variations of the micro-ELISA test with anti-human immunoglobulin G, immunoglobulin M, and Fab peroxidase-labeled conjugates revealed elevated titers with 74.3, 82.9, and 88.6% of the sera, respectively. The microagglutination and the micro-ELISA tests used in combination detected 100% of the elevated titers.
D000372:Agglutination Tests / Q000379:methods*; D000907:Antibodies, Bacterial / Q000032:analysis*; D000942:Antigens, Bacterial; D004797:Enzyme-Linked Immunosorbent Assay; D005069:Evaluation Studies as Topic; D006801:Humans; D007124:Immunoenzyme Techniques; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007877:Legionnaires' Disease / Q000175:diagnosis*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007140:Immunoglobulin Fab Fragments; D007074:Immunoglobulin G; D007075:Immunoglobulin M