[Comparison of two methods of rapid separation of serum trancobalamins (author's transl)].
We have compared different methods for the assay of unsaturated B12 binding capacity of the whole serum and of each of the three transcobalamins (TC I - TC II - TC III). This comparison was important to check the conditions of reproducibility of each method; moreover it has led us to arrive at some modifications to resolve the larger fraction (TC I + TC III) into two distinct components.
D001798:Blood Proteins / Q000302:isolation & purification*; D002244:Carbon; D002848:Chromatography, DEAE-Cellulose; D003956:Dialysis; D006454:Hemoglobins; D006801:Humans; D014155:Transcobalamins / Q000302:isolation & purification* / Q000378:metabolism; D014805:Vitamin B 12 / Q000378:metabolism
D001798:Blood Proteins; D006454:Hemoglobins; D014155:Transcobalamins; D002244:Carbon; D014805:Vitamin B 12
Confrontation de deux méthodes de séparation rapide des transcobalamines sériques.