Peanut agglutinin. I. A new tool for studying T lymphocyte subpopulations.
Fluorescein-coupled peanut agglutinin (PNA) has been used at the single-cell level to study mouse lymphocyte subpopulations. PNA not only binds to most thymocytes, as has already been shown by other authors, but also binds to a small fraction of peripheral lymphocytes that are all T cells (theta+Ig-) or null cells (theta-Ig-). Most PNA-positive thymocytes are sensitive to in vivo corticosteroids and irradiation (450 rads) treatments. Conversely, the positive spleen cells (5% of total spleen lymphocytes) are essentially resistant to corticosteroids and irradiation. Study of PNA binding during ontogenesis shows the occurrence of PNA-positive cells in the fetal liver before thymus constitution and in the very beginning of embryonic thymus and spleen development. These data indicate that PNA is a marker of early T cell subpopulations but that there are probably several distinct subsets of PNA-positive T cells.
D000818:Animals; D010367:Arachis; D001665:Binding Sites; D005260:Female; D005455:Fluorescent Antibody Technique; D006854:Hydrocortisone / Q000494:pharmacology; D037102:Lectins; D008221:Lymphoid Tissue / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D037121:Plant Lectins; D013601:T-Lymphocytes / Q000276:immunology* / Q000528:radiation effects
D037102:Lectins; D037121:Plant Lectins; D006854:Hydrocortisone