title: Nucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322.
abstract: I have determined the nucleotide sequence of the ampicillin resistance gene of pBR322, an Escherichia coli plasmid that encodes a penicillin beta-lactamase. This gene codes for a protein of 286 amino acid residues. The first 23 amino acids presumably form a signal for secretion, because they do not appear in the mature enzyme, whose partial amino acid sequence has been determined independently.
mesh_terms: D000667:Ampicillin; D001483:Base Sequence; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D010403:Penicillin Resistance; D010957:Plasmids; D001618:beta-Lactamases / Q000235:genetics
chemical_list: D000667:Ampicillin; D001618:beta-Lactamases