A simple method for maintaining relative positions of separate tissue elements during processing for electron microscopy.
Molten (328 K) 20% gelatin is used as a 'glue' to hold together separate tissue elements or tissue elements that may be separated when cutting small blocks of tissue for plastic embedding. Standard aldehyde and osmium fixation, dehydration and epoxy embedding are compatible with this as is semi-thin sectioning for light microscopy or thin sectioning for electron microscopy.
D000818:Animals; D005780:Gelatin; D006652:Histological Techniques; D051379:Mice; D008854:Microscopy, Electron; D009137:Muscular Dystrophy, Animal / Q000473:pathology; D010841:Pia Mater / Q000648:ultrastructure; D013126:Spinal Nerve Roots / Q000473:pathology
D005780:Gelatin