Measles virus nucleocapsids: large-scale purification and use in radioimmunoassays.
Nucleocapsids in quantities approaching 1 mg were purified from 109 measles virus-infected cells. They contained one polypeptide species with a molecular weight of 59,000. Antiserum was raised in rabbits against purified nucleocapsids and used in a competitive radioimmunoassay. Because of their instability, purified nucleocapsids were not suitable for use in such an assay. Instead partially purified nucleocapsids from HEp-2 cells persistently infected with measles virus and labeled in vitro with [35S]methionine were used as the source of radioactive antigen. The radioimmunoassay thus developed measured less than 5 ng of nucleocapsids in infected cells.
D000818:Animals; D000914:Antibodies, Viral; D002213:Capsid / Q000276:immunology / Q000302:isolation & purification*; D002460:Cell Line; D004591:Electrophoresis, Polyacrylamide Gel; D007163:Immunosorbent Techniques; D008459:Measles virus / Q000276:immunology / Q000302:isolation & purification* / Q000648:ultrastructure; D012367:RNA, Viral / Q000276:immunology / Q000302:isolation & purification*; D011817:Rabbits; D011863:Radioimmunoassay; D014764:Viral Proteins / Q000302:isolation & purification*
D000914:Antibodies, Viral; D012367:RNA, Viral; D014764:Viral Proteins