A pepsin-revealed material possibly related to chromosomal banding.
The enzymes pepsin, alpha-chymotrypsin, trypsin, RNase and DNase were applied to preparations of human metaphase chromosomes before staining to study whether dissociable materials related to the formation of G-, Q- and C-bands would be seen. Treatment with active pepsin but not the other enzymes revealed material with ribonucleo-protein properties which dissociated from the chromosomes and formed a halo.--Lateral extensions from the chromatids stretched to the rim of the halo and appeared at positions corresponding to G-bands. A G-band may be defined as a ring of stable chromatid-matrix binding at positions where the chromatids coil to form lateral extensions.
D002478:Cells, Cultured; D002871:Chromosome Banding; D002877:Chromosomes, Human / Q000648:ultrastructure*; D002918:Chymotrypsin / Q000494:pharmacology; D003851:Deoxyribonucleases / Q000494:pharmacology; D006801:Humans; D008214:Lymphocytes / Q000648:ultrastructure; D010434:Pepsin A / Q000494:pharmacology*; D012260:Ribonucleases / Q000494:pharmacology; D014357:Trypsin / Q000494:pharmacology
D003851:Deoxyribonucleases; D012260:Ribonucleases; D002918:Chymotrypsin; D014357:Trypsin; D010434:Pepsin A