title: An endotoxin induced serum factor that causes enhancement of antibody response to heterologous erythrocytes.
abstract: The sera obtained from blood of the mice, which had been intravenously injected with LPS several hours in advance, contained some active substance capable of enhancing anti-sheep red blood cell (SRBC) antibody responses in mice. Activity of the sera was still retained after passage through a rabbit anti-LPS antibody-coated Sepharose 4B column, but greatly reduced by passage through a rabbit anti-mouse thymocyte antibody-coated Sepharose 4B column. The active substance in the sera was eluted through a Sephadex G-200 column at the same position as the serum albumin. The addition of this substance to B cell rich spleen cell cultures in vitro in the presence of SRBC generated tremendous numbers of antibody forming cells 4 days after the incubation, suggesting that this substance was able to take over the helper function of T cells in thymus dependent antibody responses. However, this substance was not capable of stimulating 3H-thymidine-uptake into cultured spleen cells. The possible role of this substance in the adjuvant effect of LPS is discussed.
mesh_terms: D000042:Absorption; D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D000917:Antibody Formation; D002850:Chromatography, Gel; D004731:Endotoxins / Q000494:pharmacology*; D004912:Erythrocytes / Q000276:immunology*; D005260:Female; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation; D008211:Lymphocyte Cooperation; D046911:Macromolecular Substances; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008934:Mitogens / Q000494:pharmacology; D012486:Salmonella typhimurium; D012756:Sheep
chemical_list: D000276:Adjuvants, Immunologic; D004731:Endotoxins; D008070:Lipopolysaccharides; D046911:Macromolecular Substances; D008934:Mitogens