Purification of human interferons by antibody affinity chromatography.
Human Leukocyte Interferon, Human Fibroblast Interferon and Namalva-interferon was purified on an antibody affinity colomn up to 2-20 X 10 IFU/mg protein with recoveries in the 80-120% range. The colomn was made by coupling highly absorped anti-leukocyte interferon immunoglobulins to Sepharose 4B. The absorption was performed by means of crude human leukocyte interferon covalently bound to Sepharose 4B.
D000906:Antibodies; D002846:Chromatography, Affinity / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D005347:Fibroblasts / Q000276:immunology; D006801:Humans; D007372:Interferons / Q000276:immunology / Q000302:isolation & purification*; D007962:Leukocytes / Q000276:immunology
D000906:Antibodies; D007372:Interferons