title: Role of the host in virus assembly: cloning of the Escherichia coli groE gene and identification of its protein product.
abstract: Correct assembly of the heads of bacteriophages lambda and T4 requires the function of the groE gene of the Escherichia coli host. We have isolated a transducing derivative of lambda, called lambda gt-Ec.groE, that carries a functional copy of the groE gene. Unlike wild-type lambda, this phage is able to form plaques on hosts with a mutant groE gene. We have isolated an amber mutation in the groE gene carried by the phage, and this has made it possible to identify the groE product as a protein of molecular weight 65,000. In the phage, the groE gene is under the control of an early phage promoter.
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D003090:Coliphages / Q000235:genetics* / Q000378:metabolism; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D008970:Molecular Weight; D009154:Mutation; D014161:Transduction, Genetic; D014779:Virus Replication
chemical_list: D001426:Bacterial Proteins; D004274:DNA, Recombinant