The mode of condensation of aspartic acid and dihydroxyacetone phosphate in quinolinate synthesis in Escherichia coli.
Dihydroxy [3-14C]acetone phosphate was prepared enzymatically from [1-14C]glucose and use as a substrate in a partially purified quinolinate synthetase system prepared from Escherichia coli mutants. Carbon-by-carbon degradation of the resulting [14C]quinolinate showed that 96% of the 14C was located in carbon-4, indicating that carbon-3 of dihydroxyacetone phosphate condenses with carbon-3 of aspartate in quinolinate synthesis in E. coli.
D001224:Aspartic Acid / Q000378:metabolism*; D004099:Dihydroxyacetone Phosphate / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D011725:Pyridines / Q000096:biosynthesis*; D011805:Quinolinic Acids / Q000096:biosynthesis*; D014306:Trioses / Q000378:metabolism*
D011725:Pyridines; D011805:Quinolinic Acids; D014306:Trioses; D001224:Aspartic Acid; D004099:Dihydroxyacetone Phosphate