Quantation by flow microfluorometry of total cellular DNA in Acanthamoeba.
The DNA content of five species of Acanthamoeba was determined by flow microfluorometry. Acanthamoeba castellanii (AC-30), acanthamoeba polyphaga (APG and P-23), acanthamoeba rhysodes, acanthamoeba culbertsoni (A-1), and acanthamoeba royreba were grown in a casitone based medium 24-48 HR. The trophozoites were harvested, and evaluated for DNA-bound fluorescence. All species tested has DNA values between 2.0-5.0 pg/cell. These results placed DNA/cell values of Acanthamoeba slightly lower than DNA/cell values of other eucaryotic cells and much lower than Amoeba proteus values. These results indicate that FMF may be a useful adjunct in distinguishing Acanthamoeba cells from either eucaryotic cells or some other amoeba. However, differences in DNA/cell between species of Acanthamoeba are small and would not be useful in identification of species.
D000656:Amoeba / Q000032:analysis*; D000818:Animals; D001001:Anura; D002645:Chickens; D003584:Cytological Techniques; D004247:DNA / Q000032:analysis*; D004912:Erythrocytes / Q000032:analysis; D005470:Fluorometry / Q000379:methods*; D006367:HeLa Cells; D006801:Humans; D013045:Species Specificity; D014562:Caudata
D004247:DNA