Mutagenicity of methylated N-nitrosopiperidines in Saccharomyces cerevisiae.
N-Nitrosopiperidine (NP) and a number of methylated derivatives were examined for mutagenicity in Saccharomyces cerevisiae. NP, 2-methyl-NP, 3-methyl-NP, 4-methyl-NP and 3,5-dimethyl-NP were mutagens when metabolic activation (rat-liver microsomes) was provided. 2,6-Dimethyl-NP was not a mutagen. The NPs giving a positive response stimulated forward mutation to canavanine resistance (CAN1 leads to can1) and reversion of the his1-7 missense marker. Neither locus revertants nor suppressors of the lys1-1 ochre marker were induced, nor were revertants of the putative frameshift hom3-10.
D000818:Animals; D004352:Drug Resistance, Microbial; D005815:Genetic Code; D005821:Genetic Techniques; D006639:Histidine / Q000235:genetics / Q000378:metabolism; D008862:Microsomes, Liver / Q000378:metabolism; D009153:Mutagens; D009154:Mutation; D009603:Nitroso Compounds / Q000494:pharmacology*; D010443:Peptide Chain Termination, Translational; D010880:Piperidines / Q000494:pharmacology*; D051381:Rats; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000378:metabolism
D009153:Mutagens; D009603:Nitroso Compounds; D010880:Piperidines; D006639:Histidine