Immunochemical evidence of a tumor-specific surface antigen obtained by detergent solubilization of the membranes of a chemically induced sarcoma, meth-A.
We reported previously the partial purification of detergent-solubilized specific tumor rejection antigen of a chemically induced sarcoma, Meth-A. During the course of the study, rabbit antiserum against a partially purified specific tumor rejection antigen preparation was raised and rendered specific by in vivo absorption. In this report we show that an antigenic molecule defined by in vivo-absorbed rabbit antiserum, which we tentatively refer to as tumor-specific surface antigen, was solublized by detergent Nonidet P40, and extensive attempts at purification were carried out by a sequence of procedures including gel filtration, isotachophoresis, and polyacrylamide gel electrophoresis. The most highly purified tumor-specific surface antigen retained some specific tumor rejection antigen activity, suggesting an association of the two activities. Both antigens were shown to have a molecular weight of about 60,000 and an electrophoretic mobility of alpha-globulin.
D000818:Animals; D000951:Antigens, Neoplasm / Q000032:analysis / Q000302:isolation & purification; D002462:Cell Membrane / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006084:Graft Rejection; D006183:H-2 Antigens / Q000032:analysis / Q000302:isolation & purification; D007163:Immunosorbent Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D011863:Radioimmunoassay; D012513:Sarcoma, Experimental / Q000139:chemically induced / Q000276:immunology*
D000951:Antigens, Neoplasm; D006183:H-2 Antigens