[Arenavirus antigen persistence in continuous cell lines. Attempts at isolating the infectious virus].
An attempt was made to isolate an infectious virus from FL cells in which arenavirus antigen revealed by immunofluorescence tests persisted. Inhibitors of macromolecular synthesis, ultraviolet irradiation, fusion with BHK 21/13S cells, and transfection of BHK-21/13S cells with DNA from FL cells were tested in order to activate the virus. Negative results were obtained in each of these experiments. Prolonged cultivation of FL cells at 31 degres or 41 degrees C resulted in a two-fold increase in proportion of the antigen-containing cells or its complete elimination, respectively. The likelihood of integration of transcribed proviral fragment into the cell genome is discussed.
D000818:Animals; D000956:Antigens, Viral; D001116:Arenaviridae / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D002459:Cell Fusion / Q000187:drug effects; D002460:Cell Line; D002478:Cells, Cultured / Q000276:immunology*; D002642:Chick Embryo; D005455:Fluorescent Antibody Technique; D011092:Polyethylene Glycols / Q000494:pharmacology; D013696:Temperature; D014162:Transfection; D014466:Ultraviolet Rays
D000956:Antigens, Viral; D011092:Polyethylene Glycols
Persistentsiia antigena arenavirusa v perevivaemykh kletochnykh liniiakh. Popytki vydeleniia infektsionnogo virusa.