The rapid concentration and purification of influenza virus from allantoic fluid.
Influenza virus was quantitatively recovered from infectious allantoic fluid by precipitation with 8 percent (wt/vol) polyethylene glycol 6000 (PEG-6000). Virus concentrated by PEG-6000 treatment was rapidly purified by gel permeation chromatography through controlled pore glass beads. The purity of virus preparations achieved by this method, when judged by three independent criteria, was comparable to virus preparations purified by conventional density gradient procedures.
D000482:Allantois; D000818:Animals; D001826:Body Fluids / Q000382:microbiology*; D011232:Chemical Precipitation; D002642:Chick Embryo; D002850:Chromatography, Gel; D005321:Extraembryonic Membranes; D006023:Glycoproteins / Q000032:analysis; D009980:Influenza A virus / Q000302:isolation & purification* / Q000648:ultrastructure; D008828:Microbiological Techniques; D011092:Polyethylene Glycols; D014764:Viral Proteins / Q000032:analysis
D006023:Glycoproteins; D014764:Viral Proteins; D011092:Polyethylene Glycols