Correlation of the "EMIT" with a gas-liquid chromatographic method for determination of antiepileptic drugs in plasma.
We report a study of the comparison of antiepileptic drugs (phenobarbital, phenytoin, primidone, and carbamazepine) by the Enzyme Multiplied Immunoassay Technique "EMIT" and the gas chromatography (GLC) method. Overall, reasonable correlations were observed for all of the above-mentioned assays by both methods in the majority of samples included in this study. Our observations for statistical and clinical differences at various levels of phenobarbital and phenytoin are discussed. A suggestion is provided in order to avoid a major discrepancy (approximately 30% higher values by EMIT vs GLC) in results observed from the "Bottom of the Bottle Effect" for EMIT reagent.
D000927:Anticonvulsants / Q000097:blood*; D002849:Chromatography, Gas; D003201:Computers; D005069:Evaluation Studies as Topic; D006801:Humans; D007124:Immunoenzyme Techniques; D008722:Methods
D000927:Anticonvulsants