Decrease of lysosomal enzymes in macrophages incubated with supernatants of mixed culture of allograft donor and recipient lymphocytes.
Incubation of mouse peritoneal macrophages (thioglycollate-induced) for 72 hours with the supernatant of a mixed lymphocyte culture (MLC) between skin allograft donor and recipient results in a decrease of macrophage acid phosphatase (EC 3.1.3.2.) and beta glucuronidase (EC 3.2.1.31). The alteration of these lysosomal enzymes is not explained by a loss of cell viability.
D000135:Acid Phosphatase / Q000378:metabolism*; D000818:Animals; D003602:Cytotoxicity, Immunologic; D005966:Glucuronidase / Q000378:metabolism*; D066298:In Vitro Techniques; D008214:Lymphocytes / Q000276:immunology*; D008264:Macrophages / Q000201:enzymology*; D051379:Mice; D016038:Skin Transplantation; D014184:Transplantation, Homologous
D000135:Acid Phosphatase; D005966:Glucuronidase