Isolation and characterization of an actinomycin D-sensitive mutant of Saccharomyces cerevisiae.
A single mutation in Saccharomyces cerevisiae conferred sensitivity to low concentrations of actinomycin D. Treatment with actinomycin D preferentially inhibited synthesis of rRNA's. Residual rRNA synthesized was processed normally. Total protein synthesis and inducibility of the enzyme maltase were relatively unaffected at concentrations of actinomycin D which severely inhibited rRNA synthesis.
D003609:Dactinomycin / Q000494:pharmacology*; D004790:Enzyme Induction; D005656:Fungal Proteins / Q000096:biosynthesis; D005796:Genes; D009154:Mutation; D012333:RNA, Messenger / Q000096:biosynthesis; D012335:RNA, Ribosomal / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D013104:Spheroplasts / Q000187:drug effects; D000520:alpha-Glucosidases / Q000096:biosynthesis
D005656:Fungal Proteins; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D003609:Dactinomycin; D000520:alpha-Glucosidases