The mechanism of the mutagenic action of hydroxylamine XIII. Reversion of phage MS2 amber mutants in the presence of hydroxylamines.
The replication of the phage MS2 in the presence of either hydroxylamine (HA) or O-methylhydroxylamine (OMHA) (mutagenesis in vivo) results in an increase in the reversion frequency of two amber mutations in the maturation protein. When acting on the extracellular phage (mutagenesis in vitro) the mutagens do not affect the reversion frequency. The most probable mode of mutagenic action of the hydroxylamines on the vegetative MS2 phage involves the enzymic formation of modified precursors and their incorporation into RNA.
D003090:Coliphages / Q000235:genetics; D004279:DNA, Viral / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D005787:Gene Frequency; D005814:Genes, Viral; D006898:Hydroxylamines / Q000494:pharmacology*; D009153:Mutagens; D009154:Mutation / Q000187:drug effects*
D004279:DNA, Viral; D006898:Hydroxylamines; D009153:Mutagens