Ganglioside alterations in guinea pig brains at end stages of experimental Creutzfeldt-Jakob disease.
Gangliosides were isolated from guinea pig brains at the end stages of experimental Creutzfeldt-Jakob disease. Quantitative analyses revealed marked decreases of ganglioside levels in pathologically devastated tissues such as cerebral cortex (-21%), basal ganglia and thalamus (-18%), and brain stem (-23%). The cerebellum revealed only minor pathological abnormalities and its ganglioside level remained unchanged. Thin-layer chromatography of the Creutzfeldt-Jakob brain gangliosides showed aberrant ganglioside patterns in all regions studied, including the cerebellum. With some exceptions, a trend in ganglioside pattern changes was detected which consisted of proliferation of GM3, GD3, GD2 and loss of GM1, GD1a, GD1b and GT1b.
D000818:Animals; D001479:Basal Ganglia / Q000378:metabolism; D001921:Brain / Q000378:metabolism*; D001933:Brain Stem / Q000378:metabolism; D002531:Cerebellum / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism; D007562:Creutzfeldt-Jakob Syndrome / Q000378:metabolism*; D004195:Disease Models, Animal; D005732:Gangliosides / Q000032:analysis / Q000378:metabolism*; D006168:Guinea Pigs; D006801:Humans; D013788:Thalamus / Q000378:metabolism
D005732:Gangliosides