Effect on particle size of solubilization of wild-type and Re chemotype lipopolysaccharides solubilized with bovine serum albumin and triethylamine.
Disaggregation of wild-type and Re chemotype lipopolysaccharides (LPS) has been accomplished by solubilization with triethylamine followed by stabilization with bovine serum albumin in order to determine the degree of aggregation. Solubilization with 1.5% triethylamine was found to be more effective than with lower concentrations, as judged by permeation chromatography. Chromatographic analysis of triethylamine-bovine serum albumin-disaggregated LPS showed a range of particle sizes. Wild-type LPS yielded size classes of 1 X 10(6) to 4 X 10(6) daltons, 250,000 daltons, and 20,000 daltons. Re LPS yielded size classes of 1 X 10(6) to 4 X 10(6) daltons and 20,000 daltons. Untreated LPS was always greater than 4 X 10(6) daltons. Chemical characterization of the size classes revealed 3-keto-2-deoxyoctonate in all size classes and suggests that the O-antigenic side chain length may determine the aggregate size in solubilized LPS.
D002241:Carbohydrates / Q000032:analysis; D005021:Ethylamines; D007661:Ketoses; D008050:Lipid A / Q000032:analysis*; D008070:Lipopolysaccharides / Q000032:analysis*; D008970:Molecular Weight; D010316:Particle Size; D011135:Polysaccharides, Bacterial / Q000032:analysis*; D012486:Salmonella typhimurium / Q000032:analysis*; D012710:Serum Albumin, Bovine; D012995:Solubility; D013400:Sugar Acids / Q000032:analysis
D002241:Carbohydrates; D005021:Ethylamines; D007661:Ketoses; D008050:Lipid A; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D013400:Sugar Acids; D012710:Serum Albumin, Bovine; C016162:triethylamine