Measurement and synthesis of insoluble and soluble dextran by Streptococcus mutans.
Total and insoluble dextransucrase activities of 10 strains of oral streptococci were measured by a modified filter disk assay. Strains that were nonadherent to hard surfaces had only low levels of insoluble dextransucrase activity. A physical rather than metabolic mechanism is suggested to explain the decreased insoluble and increased soluble activities observed when dextran T-10 is added to the media.
D001431:Bacteriological Techniques; D003470:Culture Media; D003731:Dental Caries / Q000382:microbiology; D003910:Dextranase / Q000032:analysis; D003911:Dextrans / Q000378:metabolism* / Q000494:pharmacology; D009154:Mutation; D012995:Solubility; D013045:Species Specificity; D013295:Streptococcus mutans / Q000201:enzymology / Q000378:metabolism*; D013298:Streptococcus sanguis / Q000378:metabolism
D003470:Culture Media; D003911:Dextrans; D003910:Dextranase