title: Repression of Escherichia coli pyridine nucleotide transhydrogenase by leucine.
abstract: Addition of 0.1% casein hydrolysate to a minimal growth medium decreased membrane-bound transhydrogenase activity in Escherichia coli by about 80%. Of the amino acids added individually to the growth medium, only leucine and, to a lesser extent, methionine and alanine were effective, alpha-Ketoisocaproate- and leucine-containing peptides repressed the activity, and leucine also repressed activity in adenyl cyclase-deficient and relaxed strains. Derepression of transhydrogenase followed the removal of leucine from the growth medium and was sensitive to rifampin and chloramphenicol. A phosphoglucoisomerase-deficient strain that was forced to use the hexose monophosphate shunt exclusively had normal levels of transhydrogenase, which was repressed by leucine. Transhydrogenase activity doubled in mutants lacking either of the shunt dehydrogenases but was still repressed by leucine. In strains constitutive for the leucine biosynthetic operon, transhydrogenase was repressed by leucine but in strains livR and lst R, with leucine transport resistant to leucine repression, transhydrogenase was not repressed by leucine. These data suggest that transhydrogenase may have a function in the transport of branched-chain amino acids. In a hisT strain (which has altered leucyl-tRNA), transhydrogeanse was at a repressed level without the addition of leucine, suggesting that leucyl-tRNA may be involved in the regulation.
mesh_terms: D001693:Biological Transport, Active; D004794:Enzyme Repression; D004926:Escherichia coli / Q000201:enzymology*; D005796:Genes; D006600:Hexosephosphates / Q000378:metabolism; D007930:Leucine / Q000378:metabolism / Q000494:pharmacology*; D009154:Mutation; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D012329:RNA, Bacterial / Q000502:physiology; D012343:RNA, Transfer / Q000502:physiology
chemical_list: D006600:Hexosephosphates; D012329:RNA, Bacterial; D012343:RNA, Transfer; D009247:NADH, NADPH Oxidoreductases; D007930:Leucine