Solubilization of bovine heart-value collagen.
Complete solubilization of bovine heart-valve gollagen was obtained by using sequential pepsin digestion and extraction with dithiothreitol under non-denaturing conditions. Use of the reducing agent was the crucial step resulting in solubilization. These findings suggest that disulphide-bonded proteins may be in part responsible for the insolubility of bovine heart-valve collagen.
D000818:Animals; D002417:Cattle; D003094:Collagen / Q000302:isolation & purification*; D004229:Dithiothreitol; D004591:Electrophoresis, Polyacrylamide Gel; D006351:Heart Valves / Q000032:analysis*; D010434:Pepsin A; D012995:Solubility; D013053:Spectrophotometry
D003094:Collagen; D010434:Pepsin A; D004229:Dithiothreitol