[Template properties of the decadeoxynucleotide d(pCCACGAAACC) in the RNA polymerase system of Escherichia coli].
The decadeoxynucleotide d(pCCACGAAACC) transcription by E. coli RNA-polymerase was studied. The transcript was shown to be several times longer than the template. The oligonucleotide GpGpGpGpUp complementary to the "contact" of two neighbouring template molecules was found in the pancreatic RNase digest of the RNA-product. This fact is consistent with our hypothesis reported recently. Pentanucleotide d(pGGTTT) may funtion as a primer in the decadeoxynucleotide transcription being incorporated into RNA.
D055598:Chemical Phenomena; D002621:Chemistry; D002848:Chromatography, DEAE-Cellulose; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism; D006868:Hydrolysis; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D009711:Nucleotides / Q000378:metabolism*; D012329:RNA, Bacterial / Q000096:biosynthesis; D012260:Ribonucleases; D014158:Transcription, Genetic
D009711:Nucleotides; D012329:RNA, Bacterial; D012321:DNA-Directed RNA Polymerases; D012260:Ribonucleases
Issledovanie matrichnykh svoĭstv dekadezoksinukleotida d(pCCACGAAACC) v sisteme RNK-polimerazy Escherichia coli.