Performance characteristics of a new photometer with a moving filter tape for luminescence assay.
The performance characteristics of a new photometer that incorporated a reaction system consisting of a movable filtration tape have been studied for use with the firefly luciferase assay for adenosine 5'-triphosphate. Precision, linearity, and sensitivity are given for measuring a graded series of constant-light emitters, concentrations of adenosine 5'-triphosphate, and washed bacterial cells. Precision ranged from 1 to 10% coefficient of variation. The coefficient of correlation was 0.9985 for 7 X 10(8) to 7 X 10(3) bacteria per ml; however, the accuracy decreased at the low levels. The sample processing time was 2 min for a 1-ml sample. This instrument shows potential for many applications in quantitating small numbers of organisms, e.g., pollution, water monitoring, and clinical infection detection.
D000255:Adenosine Triphosphate / Q000032:analysis*; D001419:Bacteria / Q000302:isolation & purification; D001517:Coleoptera / Q000201:enzymology; D004926:Escherichia coli / Q000302:isolation & purification; D005069:Evaluation Studies as Topic; D049454:Firefly Luciferin / Q000378:metabolism; D008156:Luciferases / Q000378:metabolism; D049449:Luminescence; D010084:Oxidation-Reduction; D010783:Photometry / Q000295:instrumentation*
D049454:Firefly Luciferin; D000255:Adenosine Triphosphate; D008156:Luciferases