Role of the host in virus assembly: cloning of the Escherichia coli groE gene and identification of its protein product.
Correct assembly of the heads of bacteriophages lambda and T4 requires the function of the groE gene of the Escherichia coli host. We have isolated a transducing derivative of lambda, called lambda gt-Ec.groE, that carries a functional copy of the groE gene. Unlike wild-type lambda, this phage is able to form plaques on hosts with a mutant groE gene. We have isolated an amber mutation in the groE gene carried by the phage, and this has made it possible to identify the groE product as a protein of molecular weight 65,000. In the phage, the groE gene is under the control of an early phage promoter.
D001426:Bacterial Proteins / Q000235:genetics*; D003090:Coliphages / Q000235:genetics* / Q000378:metabolism; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D008970:Molecular Weight; D009154:Mutation; D014161:Transduction, Genetic; D014779:Virus Replication
D001426:Bacterial Proteins; D004274:DNA, Recombinant