title: [Homogenization of samples for the culture of mycobacteria. A comparative study of samples with and without centrifugation].
abstract: A homogenization method for sputum samples for the culture of acid-fast bacilli that omits the use of centrifugation, passage test tubes and balances is described. 10% sodium phosphate was used as decontaminating substance. This method facilitates the laboratory work since the samples so treated are incubated at 37 degrees C up to the next day when they are then cultured.  676 samples prepared according to this method were compared with samples prepared according to the standard method; a 6,21% positivity in the former compared to a 5,47% in the latter as well as a lower percentage of contamination were obtained. Contamination occurred in 9,91% of samples without centrifugation and in 14,35% of centrifuged samples.
mesh_terms: D001431:Bacteriological Techniques; D002498:Centrifugation; D006801:Humans; D009161:Mycobacterium / Q000254:growth & development* / Q000302:isolation & purification; D013183:Sputum / Q000382:microbiology*
vernacular_title: Homogeneizacón de muestras para el cultivo de las micobacterias. Estudio comparativo de muestras con centrifugación y sin ella.