title: Dextran sulfates as a contaminant of DNA extracted from concentrated viruses and as an inhibitor of DNA polymerases.
abstract: Dextran sulfate is commonly used with polyethylene glycol to concentrate viruses before extraction of their DNA. However, dextran slulfate then easily contaminated such DNA and acted as a potent inhibitor of DNA polymerases from Bacillus subtilis (III), phage PBS2, and phage T4. Dextran sulfate only weakly inhibited Micrococcus luteus and Escherichia coli DNA polymerase I preparations.
mesh_terms: D001435:Bacteriophages / Q000201:enzymology*; D003090:Coliphages / Q000201:enzymology*; D004279:DNA, Viral; D003911:Dextrans / Q000494:pharmacology*; D004926:Escherichia coli / Q000201:enzymology; D008828:Microbiological Techniques; D008837:Micrococcus / Q000201:enzymology; D019384:Nucleic Acid Synthesis Inhibitors
chemical_list: D004279:DNA, Viral; D003911:Dextrans; D019384:Nucleic Acid Synthesis Inhibitors