Immunofluorescence titres of sperm centrifuged at different speeds.
Two washing procedures were used on spermatozoa to test the effect of centrifugation on immunofluorescent staining. The rapidly centrifuged spermatozoa often exhibited a loss of acrosomal but an increase in tail antigenicity. A standard technique of sperm preparation for immunofluorescence is recommended in order to have reliable and comparable results.
D000177:Acrosome / Q000276:immunology; D000941:Antigens / Q000032:analysis; D002498:Centrifugation; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D013082:Sperm Tail / Q000276:immunology; D013094:Spermatozoa / Q000276:immunology*
D000941:Antigens