[Controlled cell culture. I. A modified perfusion chamber].
The construction of a modified perfusion chamber is presented, which can be used for a prolonged cultivation of mammalian cells and tissues, for observation of the behavior of living cells as well as for the study of different effects on these cells. The chamber is made as non-demountable of optical glass, with a diffusive barrier separating the pericellular zone from that with a perfusion medium. The scheme of the equipment for cultivation of cells and tissues in this diffusion chamber on controlling the composition of nutrient medium and gas phase is given.
D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D046508:Culture Techniques / Q000295:instrumentation; D003584:Cytological Techniques / Q000295:instrumentation*; D010477:Perfusion / Q000295:instrumentation*
D003470:Culture Media
Upravliaemoe kul'tivirovanie kletok. I. Modifitsirovannaia perfuzionnaia kamera.