Biosynthesis of lipid A. Enzymatic incorporation of 3-deoxy-D-mannooctulosonate into a precursor of lipid A in Salmonella typhimurium.
The cell envelope fraction of Salmonella typhimurium contains an enzyme system which catalyzes transfer of 3-deoxyoctulosonate (KDO) from CMP-KDO to an incomplete, KDO-deficient precursor of lipid A. The enzyme system is firmly membrane-bound, but has been solubilized by treatment with nonionic detergent at alkaline pH and partially purified. Both the particulate and partially purified fractions catalyzed formation of a single reaction product containing 2 residues of KDO. Periodate oxidation of the purified product permitted tentative identification of the KDO disaccharide structure as KDO2-4KDO.
D002462:Cell Membrane / Q000201:enzymology; D007661:Ketoses / Q000378:metabolism*; D007700:Kinetics; D008050:Lipid A / Q000096:biosynthesis* / Q000302:isolation & purification; D008070:Lipopolysaccharides / Q000096:biosynthesis*; D012486:Salmonella typhimurium / Q000378:metabolism*; D013400:Sugar Acids / Q000378:metabolism*; D014166:Transferases / Q000378:metabolism
D007661:Ketoses; D008050:Lipid A; D008070:Lipopolysaccharides; D013400:Sugar Acids; C002532:2-keto-3-deoxyoctonate; D014166:Transferases