Quantitative microscopy of enzyme reactions in tissue sections.
Microdensitometry makes it possible to measure reaction products in discrete regions of tissue sections. Single cells, or groups of cells, can be selected for measurement. In this way, it is possible to obtain quantitative data from enzyme reactions, and to relate activities to histology. Some examples are given to illustrate the application of this technique.
D000818:Animals; D002358:Cartilage, Articular / Q000201:enzymology; D003720:Densitometry / Q000295:instrumentation / Q000379:methods*; D004798:Enzymes / Q000378:metabolism*; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D006651:Histocytochemistry; D007421:Intestine, Small / Q000201:enzymology; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008099:Liver / Q000201:enzymology; D051379:Mice; D008853:Microscopy / Q000295:instrumentation; D009132:Muscles / Q000201:enzymology; D051381:Rats; D013385:Succinate Dehydrogenase / Q000378:metabolism
D004798:Enzymes; D007770:L-Lactate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D013385:Succinate Dehydrogenase