Immunocytological localization of estrogen in human mammary carcinoma cells by horseradish--anti-horseradish peroxidase complex.
Tissues from 25 female and 2 male patients of mammary carcinoma were examined by the horseradish--anti-horseradish peroxidase complex to localize the presence of estrogen. Carcinoma cells of 10 females and 2 male patients showed positive staining by this technique. The nucleus and cytoplasmic membrane showed the reaction. This method is useful in identifying the exact location of the hormones or hormone-like substances in the malignant cells and, perhaps, will be of great help in selecting the patients for management.
D000328:Adult; D000368:Aged; D001943:Breast Neoplasms / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004967:Estrogens / Q000378:metabolism*; D005260:Female; D006735:Horseradish Peroxidase; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D008875:Middle Aged
D004967:Estrogens; D006735:Horseradish Peroxidase