title: F plasmid genes involved in the production of recombination-stimulating factor, control of sensitivity to some injurious agents, and chromosome replication in Escherichia coli K-12 HfrC.
abstract: Three related F'arg+ plasmids isolated by Guyer and Clark were used to analyze some properties of strain MG751, a recipient derivative of an HfrC mutant (MG7) carrying a previously described pleiotropic mutation in the integrated F plasmid. Strains MG7 and MG751 both failed to produce recombination-stimulating factor, were sensitive to monofunctional alkylating agents and UV irradiation, and were temperature sensitive for growth and DNA synthesis. It was shown that these phenotypes are controlled by F plasmids genes (designated rsf, prt, and rep) that can be separated by deletion mutations occurring on the F plasmid.
mesh_terms: D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial / Q000096:biosynthesis; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D005838:Genotype; D008741:Methyl Methanesulfonate / Q000494:pharmacology; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D010957:Plasmids; D011995:Recombination, Genetic; D013696:Temperature; D014466:Ultraviolet Rays
chemical_list: D004269:DNA, Bacterial; D008769:Methylnitronitrosoguanidine; D008741:Methyl Methanesulfonate