[Extraction of acid alpha-glucosidase from human spleen].
An efficient method for isolation of acid alpha-glucosidase from human spleen is developed. The method involves chromatography of the enzyme on rho-aminophenyl-alpha-D-glucopyranoside covalently bound to CH-Sepharose 4B, with subsequent gel filtration on Sephadex G-200. The enzyme was homogeneous by the polyacrylamide gel electrophoresis data; it was purified about 1500-fold, as compared with the crude extract (the total yield 12.5%). Besides acid alpha-glucosidase, the preparations of alpha-L-fucosidase, alpha-D-galactosidase and beta-N-acetylglucosaminidase were isolated and purified 200-, 130- and 280-fold, respectively. The nature of interaction between acid alpha-glucosidase and immobilized rho-aminophenyl-alpha-glucopyranoside is discussed.
D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005959:Glucosidases / Q000302:isolation & purification*; D006801:Humans; D008722:Methods; D013154:Spleen / Q000201:enzymology*; D000520:alpha-Glucosidases / Q000302:isolation & purification*
D005959:Glucosidases; D000520:alpha-Glucosidases
Poluchenie kisloĭ alpha-gliukozidazy iz selezenki cheloveka.