Testing in vitro of an indirect mutagen (cyclophosphamide) with human leukocyte cultures: Activation by liver perfusion and by incubation with crude liver homogenate.
The indirect mutagen cyclophosphamide was tested in human whole blood cultures with respect to chromosome-breaking activities and suppression of mitotic indices after activation by liver perfusion and crude liver homogenates with and without cofactors. Both methods produced nearly the same effects, with the exception of liver homogenate without cofactors which had only weak metabolic activities.
D000818:Animals; D002478:Cells, Cultured; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000187:drug effects; D003520:Cyclophosphamide / Q000378:metabolism / Q000494:pharmacology*; D005821:Genetic Techniques; D006801:Humans; D008099:Liver / Q000378:metabolism; D008214:Lymphocytes / Q000648:ultrastructure; D008938:Mitosis / Q000187:drug effects; D009153:Mutagens; D051381:Rats
D009153:Mutagens; D003520:Cyclophosphamide