title: Acetate metabolism in Escherichia coli.
abstract: Levels of several intermediary metabolites were measured in cells grown in acetate medium in order to test the hypothesis that the glyoxylate cycle is repressed by phosphoenolpyruvate (PEP). Wild-type cells had less PEP than either isocitrate dehydrogenase - deficient cells (which had greater isocitrate lyase activity than the wild type) or isocitrate dehydrogenase - deficient, citrate synthase-deficient cells (which are poorly inducible). Thus induction of the glyoxylate cycle is more complicated than a simple function of PEP concentration. No correlation between enzyme activity and the level of oxaloacetate, pyruvate, or citrate was found either. Citrate was synthesized in citrate synthase-deficient mutants, possibly via citrate lyase.
mesh_terms: D000085:Acetates / Q000378:metabolism*; D002951:Citrates / Q000378:metabolism; D002952:Citric Acid Cycle; D004926:Escherichia coli / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D009154:Mutation; D010728:Phosphoenolpyruvate / Q000378:metabolism
chemical_list: D000085:Acetates; D002951:Citrates; D010728:Phosphoenolpyruvate