Partial purification of human kidney antigens.
A chromatographic method using immunoaffinity has been utilized in the search for kidney-specific antigens. After repeated absorptions by liver proteins coupled to a column of Sepharose CN-Br of a rabbit anti-human kidney serum, several antibodies remained. When these antibodies were coupled to a similar column and used for immunoaffinity chromatography, 8 kidney antigens, studied as peptides with molecular weights of 33,000, 80,000, 98,000 and 110,000-125,000 were obtained. They should not be found in similarly treated liver extracts.
D000818:Animals; D000941:Antigens / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007163:Immunosorbent Techniques; D007668:Kidney / Q000276:immunology*; D008099:Liver / Q000276:immunology; D008970:Molecular Weight; D009928:Organ Specificity; D011817:Rabbits
D000941:Antigens