title: Two-wavelength cytophotometry: the choice of the wavelengths from a practical point of view.
abstract: Methods in use for the choice of the two-wavelengths and the effect of a faulty choice of the two wavelengths in the two-wavelength cytophotometric method of Patau and Ornstein are studied by means of a computer simulation and in practice upon pararosaniline-Schiff stained nuclei. A simplification of the measuring strategy is proposed without affecting the accuracy.
mesh_terms: D000818:Animals; D001001:Anura; D002467:Cell Nucleus / Q000166:cytology; D002645:Chickens; D003201:Computers; D003584:Cytological Techniques; D004912:Erythrocytes / Q000166:cytology*; D008954:Models, Biological; D013053:Spectrophotometry / Q000379:methods*