Selective denaturation of several yeast enzymes by free fatty acids.
The denaturation of eight purified yeast enzymes, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, 3-phosphoglycerate kinase, alcohol dehydrogenase, beta-fructosidase, hexokinase and glucose-6-phosphate isomerase, promoted under controlled conditions by the free fatty acids myristic and oleic, is selective. Glucose-6-phosphate dehydrogenase (D-glucose-6-phosphate:NADP+ 1 oxidoreductase, EC 1.1.1.49) is extremely sensitive to destabilization and was studied in greater detail. Results show that chain length and degree of unsaturation of fatty acids are important to their destabilizing effect, and that ligands of the enzyme can afford protection. The denaturation process results in more than one altered form. These results can be viewed in the perspective of the possibility that amphipathic substances, and in particular free fatty acids, may play a role for enzyme degradation in vivo, by initiating steps of selective denaturation.
D000429:Alcohol Oxidoreductases / Q000037:antagonists & inhibitors; D004791:Enzyme Inhibitors; D005230:Fatty Acids, Nonesterified / Q000494:pharmacology*; D005631:Fructokinases / Q000037:antagonists & inhibitors; D005956:Glucose-6-Phosphate Isomerase / Q000037:antagonists & inhibitors; D005954:Glucosephosphate Dehydrogenase / Q000037:antagonists & inhibitors; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000037:antagonists & inhibitors; D006593:Hexokinase / Q000037:antagonists & inhibitors; D007700:Kinetics; D010734:Phosphogluconate Dehydrogenase / Q000037:antagonists & inhibitors; D010735:Phosphoglycerate Kinase / Q000037:antagonists & inhibitors; D011489:Protein Denaturation; D012441:Saccharomyces cerevisiae / Q000201:enzymology
D004791:Enzyme Inhibitors; D005230:Fatty Acids, Nonesterified; D000429:Alcohol Oxidoreductases; D010734:Phosphogluconate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D005631:Fructokinases; D006593:Hexokinase; D010735:Phosphoglycerate Kinase; D005956:Glucose-6-Phosphate Isomerase