The isolation of thirteen cloned hybrid cell lines secreting mouse strain A derived antibodies with specificity for group A streptococcal carbohydrate.
Thirteen hybrid cell lines originating from the fusion of a BALB/c myeloma with strain A spleen cells were isolated each of which secreted an antibody with specificity for the carbohydrate of group A streptococci. The cell lines were stable over many in vivo passages in (BALB/c X A/J)F1 mice and secreted large amounts of antibodies into the serum and ascitic fluid of their hosts. Seven of the hybrid cell antibodies belonged to the IgM class, five were typed as IgG2a and one as an IgA immunoglobulin.
D000818:Animals; D000918:Antibody Specificity; D002241:Carbohydrates / Q000276:immunology*; D002460:Cell Line; D002478:Cells, Cultured; D002999:Clone Cells; D006822:Hybrid Cells / Q000276:immunology*; D007070:Immunoglobulin A; D007075:Immunoglobulin M; D051379:Mice; D008805:Mice, Inbred A; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000276:immunology; D013297:Streptococcus pyogenes / Q000276:immunology*
D002241:Carbohydrates; D007070:Immunoglobulin A; D007075:Immunoglobulin M