title: Studies on the fine structural localization of zinc iodide-osmium reaction in the brain. III. Some characteristics of localization in the synaptosomes.
abstract: Synaptosomes from rat cerebral cortex were impregnated in the zinc iodide--osmium (ZIO) solution. The fine structural localization of the ZIO impregnation product was studied and, in addition, the function-dependent features of the reaction were examined after electrical stimulation or potassium chloride treatment. It was revealed that: (i) Aldehyde prefixation resulted in an increase in the number of reactive synaptic vesicles in all types of synaptosomes; (ii) Electrical stimulation decreased the number of reactive vesicles in a voltage dependent manner; (iii) Potassium chloride treatment also reduced the reactivity of vesicles; the reduction was dependent on the concentration of potassium and duration of treatment; (iv) Experimental interventions leading to the release of neurotransmitters from the synaptic vesicles and to fatigue of the nerve terminals also resulted in a decrease of the ZIO-reaction product of synaptic vesicles in a manner proportional to the strength of stimuli.
mesh_terms: D000818:Animals; D001921:Brain / Q000648:ultrastructure*; D004558:Electric Stimulation; D005260:Female; D006652:Histological Techniques; D008297:Male; D009992:Osmium; D051381:Rats; D013574:Synaptosomes / Q000648:ultrastructure*; D015032:Zinc
chemical_list: D009992:Osmium; D015032:Zinc