title: Transcription of DNA-histone complexes by yeast RNA polymerase B.
abstract: Transcription of denatured DNA complexed with histones (total, H1 or H2A/H2B/H3/H4) by yeast RNA polymerase B is investigated. Binding of histones to DNA restricts its template activity by decreasing the formation of active, heparin-resistant, RNA polymerase initiation complexes. The elongation of pre-initiated RNA on denatured DNA, complexed with histones, is possible, although resulting in somewhat shorter RNA chains. It is suggested that RNA polymerase B can elongate on a DNA strand covered with histones.
mesh_terms: D000818:Animals; D004247:DNA / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D003852:Deoxyribonucleoproteins / Q000378:metabolism*; D006493:Heparin / Q000494:pharmacology; D006657:Histones / Q000378:metabolism*; D007700:Kinetics; D009698:Nucleoproteins / Q000378:metabolism*; D012319:RNA Polymerase II / Q000378:metabolism*; D051381:Rats; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D013698:Templates, Genetic; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D003852:Deoxyribonucleoproteins; D006657:Histones; D009698:Nucleoproteins; D006493:Heparin; D004247:DNA; D012319:RNA Polymerase II; D012321:DNA-Directed RNA Polymerases