[Origin of stromal bone marrow mechanocytes according to the results of typing them by isoantigens and chromosomal markers].
The bone marrow of radiochimaeras and heterotopic bone marrow transplants were used to study the origin of precursors of the fibroblasts growing in the monolayer cultures of hemopoietic tissue. In the bone marrow explants of the (C57BL/6 X CBA) F1 mice, in which the CBA bone marrow was transplanted following the lethal irradiation, the fibroblasts grown in the colonies were of recipient origin judging by isoantigens in the reaction of indirect immunofluorescence with the anti-C57BL/6-serum. At the same time in the bone marrow explants from heterotopic transplants (CBA leads to CBA X C57BL/6) the fibroblasts grown in colonies were of donor origin. The cultures of hemopoietic cells of the bone marrow of females heterotopically transplanted in the singenic male (guinea pigs Huston) contained only fibroblasts which were of donor origin judging by sex chromosomes in the metaphase plates of dividing cells. Hence, the bone marrow precursors of fibroblasts do not depend histogenetically on hemopoietic cells and are not replaced at the expense of repopulating cells of the second partner.
D000818:Animals; D001853:Bone Marrow / Q000276:immunology; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D003601:Cytotoxicity Tests, Immunologic; D005260:Female; D005347:Fibroblasts / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D006644:Histiocytes / Q000276:immunology; D006824:Hybridization, Genetic; D007519:Isoantigens; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D011828:Radiation Chimera; D012730:Sex Chromosomes; D012737:Sex Factors; D014184:Transplantation, Homologous
D007519:Isoantigens
Proiskhozhdenie stromal'nykh mekhanotsitov kostnogo mozga po dannym ikh tipirovaniia po izoantigenam i khromosomnym markeram.