Stable isotope studies on the biosynthesis of the thiazole moiety of thiamin in Escherichia coli.
Deuterated and 13C-labeled sugars were fed to Escherichia coli growing on defined medium. The position and extent of incorporation of the label into the 4-methyl-5-beta-hydroxyethylthiazole portion of thiamin and other cellular components were measured by gas chromatography-mass spectrometry. Based on the findings, it is concluded that the contiguous five-carbon unit of the 4-methyl-5-beta-hydroxyethylthiazole is biosynthetically derived from pyruvate and a triose phosphate.
D002247:Carbon Isotopes; D003903:Deuterium; D004926:Escherichia coli / Q000378:metabolism*; D007553:Isotope Labeling / Q000379:methods; D013831:Thiamine / Q000096:biosynthesis*; D013844:Thiazoles
D002247:Carbon Isotopes; D013844:Thiazoles; D003903:Deuterium; D013831:Thiamine