title: The configuration and location of the ribosidic linkage in the prosthetic group of citrate lyase (Klebsiella aerogenes).
abstract: The structure of the prosthetic group of citrate lyase (Klebsiella aerogenes) was studied by nuclear magnetic resonance and mass spectrometry. The spectra at 360 MHz of the nucleoside moiety (2'-ribosyladenosine) show the absence of 2'-hydroxyl proton, thus confirming the 2' position as the site of attachment of the second ribose moiety to the dephospho-CoA. This glycosidic linkage is found to be alpha(1" leads to 2') and is identical to that of poly(ADP-ribose). Studies of permethylation products by mass spectrometry support the above conclusion regarding the location of the ribosidic linkage.
mesh_terms: D001275:ATP Citrate (pro-S)-Lyase; D000241:Adenosine; D006027:Glycosides / Q000032:analysis; D007711:Klebsiella pneumoniae / Q000201:enzymology*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008968:Molecular Conformation; D012266:Ribose / Q000032:analysis
chemical_list: D006027:Glycosides; D012266:Ribose; D001275:ATP Citrate (pro-S)-Lyase; D000241:Adenosine