Evidence for catabolite degradation in the glucose-dependent inactivation of yeast cytoplasmic malate dehydrogenase.
The cytoplasmic malate dehydrogenase of Saccharomyces cerevisiae was radioactively labeled during its synthesis on a glucose-free derepression medium. After purification a sensitive radio-immunoassay for this enzyme could be developed. The assay showed that after the physiological, glucose-dependent 'catabolite inactivation' of cytoplasmic malate dehydrogenase an inactive enzyme protein is immunologically not detectable. Together with the irreversibility of this reaction in vivo this finding strongly suggest a proteolytic mechanism of enzyme inactivation. For this process the term 'catabolite degradation' is used.
D000918:Antibody Specificity; D003593:Cytoplasm / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology*; D008291:Malate Dehydrogenase / Q000032:analysis / Q000037:antagonists & inhibitors* / Q000276:immunology; D008928:Mitochondria / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism; D011863:Radioimmunoassay; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
D008291:Malate Dehydrogenase; D010447:Peptide Hydrolases; D005947:Glucose