title: Microbiological method for assaying lincomycin in animal feed: collaborative study.
abstract: A microbiological assay for determining lincomycin in swine feed, supplement, and a vitamin-mineral premix was studied collaboratively in 16 laboratories. The design of the study involved a complete feed, feed supplement, and a vitamin-mineral premix covering a range of fortification from 20 to 80 g/ton and 80 to 2600 g/ton. Two methods of sample preparation were used depending on the concentration of lincomycin in the sample. Statistical evaluation of the results from the 2 methods indicated that 10 and 11 collaborators, respectively, had mean recoveries which were not significantly different from one another. Ten laboratories obtained a mean recovery of 112.2% (range 102.3--123.5%) for the lower level, and 11 laboratories obtained a mean recovery of 104.4% (range 100.0--107.7%) for the higher level. The method has been adopted as official first action.
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D008034:Lincomycin / Q000032:analysis*; D008828:Microbiological Techniques; D013552:Swine
chemical_list: D008034:Lincomycin