Lysosomes in normal pancreatic beta cells.
Lysosomes and their relationships with surrounding organelles were studied in pancreatic B-cells of normal Wistar rats by electron microscopy and cytochemical localization of acid phosphatase (AcPase) and arylsulphatase. Several forms of lysosomes were distinguished and shown to interact frequently with the secretory granules. Two different digestive processes were recognized. During crinophagy, lysosomes directly fuse with secretory granules while, during autophagy, a cytoplasmic area containing secretory granules and/or other organelles is circumscribed before the enzymatic digestion. In addition, lysosomes may transport arylsulphatase into secretory granules apparently not involved in a destructive process. Such a process could also account, at least in part, for the presence of AcPase in a certain number of mature and immature secretory granules.
D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D001192:Arylsulfatases / Q000378:metabolism; D007515:Islets of Langerhans / Q000166:cytology*; D008247:Lysosomes / Q000201:enzymology; D008297:Male; D051381:Rats
D000135:Acid Phosphatase; D001192:Arylsulfatases