Positive control of D-serine deaminase synthesis in vitro.
Efficient constitutive synthesis of D-serine deaminase [D-serine hydro-lyase (deaminating); EC 4.2.1.14] is obtained in vitro by using a slightly modified Zubay system programmed with dsdO6 dsdA+DNA. Synthesis from a dsdO+ dsdA+ template requires active dsdC gene product and 3':5'-cyclic AMP. In vitro synthesis of dsdC product is obtained with a dsdC+ dsdO+ dsdA+ or a dsdCc dsdO+ dsdA+ template; this synthesis is thermosensitive and can be uncoupled from D-serine deaminase synthesis by temperature shift.
D002474:Cell-Free System; D003090:Coliphages / Q000235:genetics; D004790:Enzyme Induction; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D005796:Genes; D005809:Genes, Regulator; D012695:L-Serine Dehydratase / Q000096:biosynthesis / Q000235:genetics*; D009876:Operon; D012694:Serine / Q000494:pharmacology; D013698:Templates, Genetic
D012694:Serine; D012695:L-Serine Dehydratase