Suppression of Hela cell growth and increase in nuclear size by chemical carcinogens: a possible screening method.
HeLa cell monolayers were "pulse" treated with either carcinogenic or "non-carcinogenic" chemicals. Pre-carcinogens were added with a liver homogenate to provide an appropriate metabolizing system. All proximate carcinogens and a proportion of pre-carcinogens were able to inhibit cell division, and in all cases examined, this was accompanied by nuclear enlargement. Although several "non-carcinogenic" chemicals also arrested cell division, nuclear enlargment was not produced. The possibility that growth inhibition and nuclear enlargement in cells treated briefly with a chemical could provide a rapid indication of carcinogenic activity is discussed.
D002273:Carcinogens / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects*; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D005821:Genetic Techniques; D006367:HeLa Cells / Q000166:cytology; D006801:Humans
D002273:Carcinogens