Enzyme-linked immunosorbent assay for detection of Clostridium botulinum toxin type A.
The enzyme linked immunosorbent assay using the so-called "double-sandwich technique" has been applied to determine botulinum toxin type A. By this assay, 50-100 mouse ip LD50 of toxin type A can be detected. No cross-reaction occurs with botulinum toxins of other types tested. In all probability this is due to the high specificity of the antiserum prepared against the toxic component of type A toxin.
D000818:Animals; D001905:Botulinum Toxins / Q000032:analysis* / Q000276:immunology; D003014:Clostridium botulinum / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D007124:Immunoenzyme Techniques; D007928:Lethal Dose 50; D011817:Rabbits
D001905:Botulinum Toxins