An "antibody electrode," preliminary report on a new approach in enzyme immunoassay.
We report preliminary experiments on a new, sensitive, and reliable procedure for enzyme immunoassay of various antigens in biological fluids. The method, developed from the biological model "hepatitis B surface-antigen/antibodies," is less time consuming than most immunochemical techniques and eliminates many inconveniences arising from use of isotopes. We use a solid-phase "sandwich" procedure, the antibodies being immobilized on gelatin membranes, and determine antigen concentration with the help of an iodide-sensitive electrode modified by fixing the active membrane onto the crystal sensor. We have established the analytical criteria of the method and compared it with the solid-phase radioimmunoassay for the surface antigen in dilution series. One-tenth microgram of antigen per liter can be reproducibly detected with our method. Use of antibody electrode should easily be extended to assay of other antigens and haptens that usually are determined by radioimmunoassay.
D000818:Animals; D000906:Antibodies; D004566:Electrodes; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D006735:Horseradish Peroxidase; D007124:Immunoenzyme Techniques; D011817:Rabbits / Q000276:immunology
D000906:Antibodies; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D006735:Horseradish Peroxidase