title: An assessment of the radial diffusion method for the measurement of pepsin in gastric secretion and its comparison with the haemoglobin substrate colorimetric method.
abstract: The radial diffusion method of pepsin assay has been compared with a modified Anson-Mirsky colorimetric method. Though claimed to measure accurately over a wide range, we found that the assay range of the radial diffusion method was only 10 to 100 mg/l; even with these narrow limits, it was inaccurate. In contrast, the colorimetric method was much more rapid, sensitive and could accurately measure a wide range of 0.5 to 500 mg/l. The colorimetric technique is therefore the superior method.
mesh_terms: D004058:Diffusion; D005069:Evaluation Studies as Topic; D005750:Gastric Juice / Q000201:enzymology*; D006454:Hemoglobins / Q000378:metabolism; D008027:Light; D010434:Pepsin A / Q000378:metabolism*; D013053:Spectrophotometry
chemical_list: D006454:Hemoglobins; D010434:Pepsin A