Use of matrix-immobilised recombinant plasmids to purify chain-specific rabbit globin complementary DNAs.
The cloning of DNA sequences in plasmid recombinants has made it possible to amplify specific sequences to an extent that they can be used for preparative purposes. We describe the use of rabbit globin DNA sequences cloned in the plasmid pCR1 and covalently bound to Sepharose 4B for the purification of chain-specific rabbit alpha- and beta-globin cDNAs. These purified probes were then used to estimate the length of the alpha- and beta-globin DNA sequences inserted into the recombinant plasmid. The technique should allow the rapid isolation of sequence-specific cDNA, RNA and genomic DNA.
D000818:Animals; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004247:DNA / Q000302:isolation & purification*; D004274:DNA, Recombinant / Q000235:genetics*; D004926:Escherichia coli; D005914:Globins / Q000235:genetics*; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits
D004274:DNA, Recombinant; D012333:RNA, Messenger; D005914:Globins; D004247:DNA