Standardized fixation of human lung for radiology and morphometry; Description of a "two chamber"-system with formaldehyde vapor inflation.
In order to obtain optimal preservation of lungs for histology, morphometry and radiology a two-chamber system of lung fixation with hot formaldehyde vapor is introduced. Compared with other procedures this system offers small dead space and ensures quick and thorough fixation by imitating "inspiration" and "expiration". Volume- and pressure-regulated "breathing" enables fixation in a stage of the respiration cycle, which can be chosen. This procedure can be used routinely in the autopsy room. The only serious drawback seems to be air pollution, if the chambers are not absolutely insulated.
D000397:Air Pollution; D001344:Autopsy; D005557:Formaldehyde; D006652:Histological Techniques; D006801:Humans; D008168:Lung / Q000000981:diagnostic imaging; D008722:Methods; D011859:Radiography; D012119:Respiration
D005557:Formaldehyde