title: Pathogenic effect of enterotoxigenic Escherichia coli and Escherichia coli causing infantile diarrhoea.
abstract: Macroscopic, light and electron microscopic alterations in ligated rabbit intestinal loops challenged with five standard enterotoxigenic Escherichia coli (ETEC) and twenty-three enteropathogenic E. coli (EEC-I) strains, freshly isolated from infantile enteritis cases, were investigated. Only two O26 : K60 : H11 strains produced enterotoxin. Their living cultures, sterile filtrates of the fluid medium and ultrasonic lysates of the bacteria resulted in pronounced hypersecretion of the intestinal epithelium followed by fluid accumulation and loop dilatation. These two E. coli strains, similarly as the other loop-negative EEC-I strains, were able to penetrate into the intestinal epithelium. In contrast to the standard ETEC strains, the EEC-I bacteria, adhering to the brush border, intruded into the microvilli, multiplied on the outer epithelial cell membrane making close contact with it and, causing, shedding of microvilli, penetrated into enterocytes becoming enclosed in membrane-bound phagosome-like vacuoles, appeared in the lamina propria and elicited mild focal polymorphonuclear infiltration.
mesh_terms: D000818:Animals; D003968:Diarrhea, Infantile / Q000382:microbiology*; D004768:Enterotoxins / Q000096:biosynthesis*; D004926:Escherichia coli / Q000378:metabolism / Q000472:pathogenicity*; D004927:Escherichia coli Infections; D007422:Intestines / Q000382:microbiology / Q000648:ultrastructure; D007428:Intradermal Tests; D051379:Mice; D011817:Rabbits
chemical_list: D004768:Enterotoxins