Evidence for the involvement of an energy-dependent process in gonadotropin-releasing hormone-stimulated luteinizing hormone release by rat anterior pituitary.
The involvement of an energy-dependent process in gonadotropin-releasing hormone-(GnRH) stimulated LH release in the anterior pituitary has been examined. Inhibitors of both glycolysis and oxidative phosphorylation inhibited stimulation by GnRH of LH release in a time- and dose-dependent manner. Iodoacetate and 2-deoxyglucose inhibited GnRH-stimulated LH release, and this inhibition was not overcome by the addition of pyruvate. Similarly carbonyl cyanide m-chlorophenylhydrazine, an uncoupler of oxidative phosphorylation, and sodium azide, an inhibitor of electron transport, decreased ATP levels, enhanced lactate accumulation, and inhibited GnRH-stimulated LH release. These studies demonstrate that inhibition of glycolysis and oxidative phosphorylation with metabolic inhibitors lead to inhibition of GnRH-stimulated LH release. Based on these results, it is postulated that GnRH-stimuoated LH release is dependent on an energy-dependent process.
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001386:Azides / Q000494:pharmacology; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology*; D003847:Deoxyglucose / Q000494:pharmacology; D004579:Electron Transport; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology*; D007461:Iodoacetates / Q000494:pharmacology; D007700:Kinetics; D007773:Lactates / Q000378:metabolism; D007986:Luteinizing Hormone / Q000378:metabolism*; D009570:Nitriles / Q000494:pharmacology*; D010085:Oxidative Phosphorylation / Q000187:drug effects; D010903:Pituitary Gland, Anterior / Q000187:drug effects / Q000378:metabolism*; D051381:Rats
D001386:Azides; D007461:Iodoacetates; D007773:Lactates; D009570:Nitriles; D007987:Gonadotropin-Releasing Hormone; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D000255:Adenosine Triphosphate; D007986:Luteinizing Hormone; D003847:Deoxyglucose