A method for enumeration ot T and B lymphocytes in tissues.
A method for the identification of T and B lymphocytes in tissue specimens is described. A sonication technique results in viable relatively pure lymphocyte populations which are easily classified by their surface markers. This readily reproducible method can become a standard laboratory procedure in the evaluation of disease states which require such information related to the classification of lymphocyte cell origin.
D000328:Adult; D000368:Aged; D001402:B-Lymphocytes / Q000166:cytology* / Q000648:ultrastructure; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008198:Lymph Nodes / Q000473:pathology; D008223:Lymphoma / Q000473:pathology; D008297:Male; D008722:Methods; D008875:Middle Aged; D013010:Sonication; D013154:Spleen / Q000166:cytology; D013601:T-Lymphocytes / Q000166:cytology* / Q000648:ultrastructure; D013950:Thymus Gland / Q000166:cytology