Introduction of bacteriophage lambda into cells of Klebsiella aerogenes.
We have shown that a mutation in the cro gene of phage lambda greatly reduces zygotic induction. This observation has allowed us to move this phage on an episome into cells of Klebsiella aerogenes where it grows as well as in cells of Escherichia coli. This technique should allow the introduction of various derivatives of lambda into any organism which is able to receive deoxyribonucleic acid from E. coli.
D003090:Coliphages / Q000235:genetics* / Q000254:growth & development; D005814:Genes, Viral; D007711:Klebsiella pneumoniae / Q000235:genetics*; D009154:Mutation; D010957:Plasmids; D011995:Recombination, Genetic