Improved method for the specific immunoprecipitation of albumin.
The immunoprecipitates of many antigens are frequently contaminated by coprecipitation of unrelated substances. A method to overcome this type of contamination in the immunoprecipitation of albumin is described. The insoluble albumin-antibody complexes are solubilized by a brief treatment at high temperature in the presence of sodium dodecyl sulfate, and after dilution the mixture is submitted to a second immunoprecipitation.
D000818:Animals; D000937:Antigen-Antibody Reactions; D000941:Antigens / Q000302:isolation & purification*; D003902:Detergents / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D006358:Hot Temperature; D007163:Immunosorbent Techniques; D051381:Rats; D012709:Serum Albumin / Q000276:immunology* / Q000302:isolation & purification; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology
D000941:Antigens; D003902:Detergents; D012709:Serum Albumin; D012967:Sodium Dodecyl Sulfate