A technique for the purification of immune complexes using rheumatoid factor.
A method for the purification of immune complexes (IC) from human serum is described, using as a model complexes of tetanus toxoid with human antitoxoid antibodies (TAT). The technique is based on the ability of IC to bind to tubes coated with rheumatoid factor (RF). Small amounts of TAT IC were added to human serum and the mixtures were rotated overnight in tubes coated with RF. The tubes were then washed and the bound material was eluted with sodium dodecyl sulphate and iodinated with 125I. Analysis of the labeled preparation by electrophoresis in polyacrylamide gels revealed the presence of the toxoid component. The technique should be useful in isolating small amounts of IC for analytical purposes.
D000906:Antibodies; D000915:Antibody Affinity; D000918:Antibody Specificity; D000936:Antigen-Antibody Complex; D001666:Binding Sites, Antibody; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007158:Immunologic Techniques; D008722:Methods; D008970:Molecular Weight; D012217:Rheumatoid Factor; D013745:Tetanus Toxoid / Q000276:immunology
D000906:Antibodies; D000936:Antigen-Antibody Complex; D013745:Tetanus Toxoid; D012217:Rheumatoid Factor