title: The prostaglandins system and insulin release. Studies with the isolated perfused rat pancreas.
abstract: Using the isolated perfused rat pancreas PGE2 (1 MUM and 10 muM) had no effect on basal or glucose (10 and 20 mM)-induced insulin release (IR). PGF2 alpha stimulated basal IR at 1 muM and inhibited IR at 10 muM. The glucose-induced IR was unaffected by this PG. Furosemide (5 and 10 mM) led to a monophastic IR at low glucose (glu) and to a potentiation of IR at high glu. Only high indomethacin (Indo) (50 microgram/ml) inhibited glu-induced IR. The stimulatory effect of furosemide on IR could not be inhibited by indomethacin. However mepacrine (0.1 mM) abolished the furosemide effect. Also glu-induced IR was inhibited by mepacrine. Acetylsalicylic acid (30 mg/100 ml) had no significant influence on glu-induced IR. These findings provide evidence that phospholipase activation rather than increased PG synthesis might primarily be involved in the secretory process of insulin.
mesh_terms: D000818:Animals; D001241:Aspirin / Q000494:pharmacology; D005665:Furosemide / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology; D007213:Indomethacin / Q000494:pharmacology; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000378:metabolism*; D008297:Male; D011453:Prostaglandins / Q000502:physiology*; D011458:Prostaglandins E / Q000494:pharmacology*; D011460:Prostaglandins F / Q000494:pharmacology*; D011796:Quinacrine / Q000494:pharmacology; D051381:Rats
chemical_list: D007328:Insulin; D011453:Prostaglandins; D011458:Prostaglandins E; D011460:Prostaglandins F; D005665:Furosemide; D011796:Quinacrine; D005947:Glucose; D001241:Aspirin; D007213:Indomethacin