Characterization of an Escherichia coli K12 mutant that is sensitive to chlorate when grown aerobically.
Escherichia coli can normally grow aerobically in the presence of chlorate; however, mutants can be isolated that can no longer grow under these conditions. We present here the biochemical characterization of one such mutant and show that the primary genetic lesion occurs in the ubiquinone-8-biosynthetic pathway. As a consequence of this, under aerobic growth conditions the mutant is apparently unable to synthesize formate dehydrogenase, but can synthesize a Benzyl Viologen-dependent nitrate reductase activity. The nature of this activity is discussed.
D000166:Acridines / Q000378:metabolism; D000332:Aerobiosis; D002704:Chlorates / Q000494:pharmacology*; D003580:Cytochromes / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D005561:Formates / Q000378:metabolism; D009154:Mutation; D009250:NADP Transhydrogenases / Q000378:metabolism; D009565:Nitrate Reductases / Q000378:metabolism; D011809:Quinones / Q000378:metabolism; D013050:Spectrometry, Fluorescence
D000166:Acridines; D002704:Chlorates; D003580:Cytochromes; D005561:Formates; D011809:Quinones; D009250:NADP Transhydrogenases; D009565:Nitrate Reductases