title: Low ribonuclease activity in cellular lysates of osmotic sensitive Saccharomyces cerevisiae mutants.
abstract: Cellular lysates with very low total ribonuclease activities are obtained by lysis of Saccharomyces cerevisiae VY1160 osmotic sensitive mutant cells in 1% sorbitol solution. These lysates could be used for isolation of intact polysomes and messenger RNA molecules, or for studying of specific ribonucleases.
mesh_terms: D002458:Cell Fractionation; D009154:Mutation; D009996:Osmotic Fragility; D012331:RNA, Fungal; D012260:Ribonucleases / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*
chemical_list: D012331:RNA, Fungal; D012260:Ribonucleases