title: In vitro packaging of exogenous DNA by Salmonella phage P22.
abstract: We describe in vitro conditions for packaging of exogenous DNA of Salmonella phage P22 which has terminally redundant, circularly permuted DNA. The method is a modification of the Kaiser-Masuda procedure. The most important aspect is to prepare all components (proheads, enzymes and concatemeric DNA) in end- cells. The influence of several factors such as DNA- and Mg2+ concentration and kinetics has been investigated.
mesh_terms: D000255:Adenosine Triphosphate / Q000494:pharmacology; D002474:Cell-Free System; D004279:DNA, Viral; D008274:Magnesium / Q000494:pharmacology; D008828:Microbiological Techniques; D012483:Salmonella Phages / Q000254:growth & development*; D012486:Salmonella typhimurium; D013095:Spermidine / Q000494:pharmacology; D014779:Virus Replication / Q000187:drug effects
chemical_list: D004279:DNA, Viral; D000255:Adenosine Triphosphate; D008274:Magnesium; D013095:Spermidine