Production of pyrogenic exotoxin by groups of streptococci: association with group A.
Several groups of streptococci were tested for production of pyrogenic exotoxins (SPE) with Ouchterlony immunodiffusion, a newly developed passive hemagglutination inhibition assay, and an assay for pyrogenicity and capacity to enhance lethal endotoxin shock. With use of these assays, 68 (91%) of 75 group A streptococcal strains were positive for one or more of SPE types A, B, and C; seven were negative for both the known SPE types and antigenically unrelated pyrogenic exotoxins. Group A strains producing both SPE B and C were the most common, and strains producing A alone or AB and AC together were the least common. All of 11 rheumatogenic group A streptococci elaborated SPE C either alone or together with one or both of SPE types A and B. The 10 nephritogenic strains tested were positive for SPE B; five were positive for B alone. In contrast to group A streptococci, non-group A strains (41 tested) did not produce the known SPE types, and 19 of 19 tested were negative for antigenically unrelated pyrogenic exotoxins. Group A strains from Holland, India, and Japan also elaborated SPE. Several group A streptococci used widely in laboratory experiments were tested for SPE types produced.
D000818:Animals; D009202:Cardiomyopathies / Q000139:chemically induced; D005098:Exotoxins / Q000096:biosynthesis* / Q000494:pharmacology; D006385:Hemagglutination Inhibition Tests; D006801:Humans; D009393:Nephritis / Q000139:chemically induced; D011749:Pyrogens / Q000096:biosynthesis* / Q000494:pharmacology; D011817:Rabbits; D012216:Rheumatic Diseases / Q000139:chemically induced; D012772:Shock, Septic / Q000139:chemically induced; D013292:Streptococcus agalactiae / Q000276:immunology; D013297:Streptococcus pyogenes / Q000276:immunology*
D005098:Exotoxins; D011749:Pyrogens