Rapid methods for generalized transduction of Salmonella typhimurium mutants.
A procedure has been developed that allows the propagation of generalized transducing phage directly on cells growing on solid media. After the donor cells are killed with chloroform, the phage can be transferred directly to recipient cells and transductants can be selected.
D001431:Bacteriological Techniques; D005796:Genes; D009154:Mutation; D011995:Recombination, Genetic; D012483:Salmonella Phages; D012486:Salmonella typhimurium / Q000235:genetics*; D014161:Transduction, Genetic