title: A study of Klebsiella pneumoniae infection in the uterus of the mare.
abstract: Two experiments incorporating 13 mares were conducted for the purpose of producing and monitoring intrauterine infection with Klebsiella pneumoniae. In the pilot study, the infection was produced with strains of K pneumoniae type 68 and type 10 isolated from the genital tract of stallions with a history of breeding problems. In the principal study, K pneumoniae type 68 was used to produce the infection. Tampons and guarded culture swabs were used to obtain uterine samples in the pilot study. In comparing the efficacies of isolation of K pneumoniae with the tampons and isolation with standard guarded culture swab, the tampon proved to be a more reliable means with which to isolate K pneumoniae and was used in the principal study. In both studies, inoculated mares became infected and remained infected at least until the postinoculation estrous cycle was initiated or was completed. Some of the inoculated mares remained infected through more than one estrous cycle. The numbers of K pneumoniae decreased in the uterus of mares after completing the estrous cycle after inoculation. Klebsiella pneumoniae was not demonstrable in frozen tissue sections of uterine biopsy specimens stained by fluorescent antibody technique. Postinoculation sera antibody titers to K pneumoniae, as determined, using the capsule swelling technique, were no higher than 1:8.
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D003470:Culture Media; D004971:Estrus; D005260:Female; D006734:Horse Diseases / Q000382:microbiology*; D006736:Horses; D007710:Klebsiella Infections / Q000382:microbiology / Q000662:veterinary*; D007711:Klebsiella pneumoniae / Q000276:immunology / Q000302:isolation & purification; D008297:Male; D011247:Pregnancy; D014591:Uterine Diseases / Q000382:microbiology / Q000662:veterinary*; D014599:Uterus / Q000378:metabolism / Q000382:microbiology
chemical_list: D000907:Antibodies, Bacterial; D003470:Culture Media