[Spontaneous transformation and long term culture of foetal mouse glial cells (author's transl)].
Six cell lines originated from foetal mouse brain were maintained in vitro for more than two years. The morphology of most of these cultures suggests their glial character. This is corroborated, for some lines, by the presence of specific glial protein (Glial fibrillary acidic protein, or GFAP). Five out of these lines remained not transplantable in animals during all the experiments. However one of them went through a spontaneous transformation at the eleventh population doubling. This culture, after transformation remained glial and when injected in animals, induced GFAP containing tumors.
D000818:Animals; D001921:Brain / Q000166:cytology / Q000196:embryology*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D005260:Female; D051379:Mice; D008858:Microscopy, Phase-Contrast; D009368:Neoplasm Transplantation; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009457:Neuroglia / Q000166:cytology* / Q000378:metabolism
D009419:Nerve Tissue Proteins
Transformation spontanée et maintien prolongé in vitro de cellules gliales d'embryons de souris.