Expression in Escherichia coli of hepatitis B virus DNA sequences cloned in plasmid pBR322.
Fragments of hepatitis B virus DNA isolated from Dane particles have been inserted into the Escherichia coli plasmid pBR322 and cloned. Cells carrying the hybrid plasmid synthesise antigenic material that reacts specifically with antisera to hepatitis B viral antigens.
D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004270:DNA, Circular / Q000235:genetics; D004274:DNA, Recombinant; D004279:DNA, Viral / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005814:Genes, Viral; D006515:Hepatitis B virus / Q000235:genetics*; D010957:Plasmids
D004270:DNA, Circular; D004274:DNA, Recombinant; D004279:DNA, Viral; D004262:DNA Restriction Enzymes