Cold-stable microtubules in the cytoplasm of mouse embryo fibroblasts.
Treatment of cultured mouse embryo fibroblasts with Triton X-100 after prolonged incubation at 0 degrees C reveals a network of microtubules in the cytoplasm of cooled cells. This network of cold-stable microtubules was demonstrated by immunofluorescence microscopy, using a monospecific antibody against tubulin and by electron microscopy. The cold-stable microtubules, as well as the ordinary cytoplasmic microtubules, were sensitive to Ca ions and were not observed in the cells pre-treated with colchicine or vinblastine. The cold-stable microtubules do not seem to be in equilibrium with the pool of depolymerized tubulin at 0 degrees C.
D000818:Animals; D002460:Cell Line; D003078:Colchicine / Q000494:pharmacology; D003080:Cold Temperature; D003593:Cytoplasm / Q000648:ultrastructure; D005347:Fibroblasts / Q000187:drug effects / Q000648:ultrastructure*; D005455:Fluorescent Antibody Technique; D051379:Mice; D008870:Microtubules / Q000187:drug effects / Q000648:ultrastructure*; D011092:Polyethylene Glycols / Q000494:pharmacology; D014404:Tubulin / Q000276:immunology; D014747:Vinblastine / Q000494:pharmacology
D014404:Tubulin; D011092:Polyethylene Glycols; D014747:Vinblastine; D003078:Colchicine