In vitro packaging of exogenous DNA by Salmonella phage P22.
We describe in vitro conditions for packaging of exogenous DNA of Salmonella phage P22 which has terminally redundant, circularly permuted DNA. The method is a modification of the Kaiser-Masuda procedure. The most important aspect is to prepare all components (proheads, enzymes and concatemeric DNA) in end- cells. The influence of several factors such as DNA- and Mg2+ concentration and kinetics has been investigated.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D002474:Cell-Free System; D004279:DNA, Viral; D008274:Magnesium / Q000494:pharmacology; D008828:Microbiological Techniques; D012483:Salmonella Phages / Q000254:growth & development*; D012486:Salmonella typhimurium; D013095:Spermidine / Q000494:pharmacology; D014779:Virus Replication / Q000187:drug effects
D004279:DNA, Viral; D000255:Adenosine Triphosphate; D008274:Magnesium; D013095:Spermidine