Determination of blood urea by gas-liquid chromatography. A comparison with two other methods.
An original method for the quantitative determination of plasmatic urea by gas-liquid chromatography (GLC) is described. It is based upon the transformation of urea into urethane by alcoholic deamination in a warm and strictly anhydrous medium. Compared with the two other usual methods (enzymatic and colorimetric), the GLC technique is extremely reliable and specific, and can easily be adapted to biological fluids.
D002849:Chromatography, Gas; D003124:Colorimetry; D003641:Deamination; D006801:Humans; D014508:Urea / Q000097:blood* / Q000378:metabolism; D014510:Urease; D014520:Urethane / Q000032:analysis
D014520:Urethane; D014508:Urea; D014510:Urease