A sensitive hemolytic assay for mouse C3.
We have developed a sensitive hemolytic assay for mouse C3, utilizing readily available guinea pig complement components and standard techniques, which allows C3 to be detected in mouse serum diluted many thousand-fold. Mouse serum also contains a potent, relatively heat-stable C3-inactivator system, which is largely blocked by Suramin.
D000818:Animals; D003176:Complement C3 / Q000302:isolation & purification*; D004546:Elapid Venoms / Q000494:pharmacology; D005260:Female; D006462:Hemolytic Plaque Technique; D006358:Hot Temperature; D051379:Mice; D008815:Mice, Inbred Strains / Q000097:blood*; D009994:Osmolar Concentration; D013498:Suramin / Q000494:pharmacology; D013997:Time Factors
D003176:Complement C3; D004546:Elapid Venoms; D013498:Suramin