Mechanism of conjugation. II. Characterization of an Hfr dna ts mutant of Escherichia coli K-12.
A new conditional thermosensitive Hfr mutant of Escherichia coli K-12 was isolated. The ts mutation is cotransducible with purE and tsx loci on the E. coli chromosome. Upon temperature shift to 42 degrees C the DNA synthesis and transfer of chromosome is stopped immediately and RNA, protein synthesis in about ten minutes.
D001426:Bacterial Proteins / Q000096:biosynthesis; D003227:Conjugation, Genetic; D004261:DNA Replication; D004926:Escherichia coli / Q000235:genetics*; D006358:Hot Temperature; D009154:Mutation; D012329:RNA, Bacterial / Q000096:biosynthesis
D001426:Bacterial Proteins; D012329:RNA, Bacterial