title: Conditional-lethal deoxyribonucleic acid ligase mutant of Escherichia coli.
abstract: A new Escherichia coli deoxyribonucleic acid (DNA) ligase mutant has been identified among a collection of temperature-sensitive DNA replication mutants isolated recently (Sevastopoulos, Wehr, and Glaser, Proc. Natl. Acad. Sci. U.S.A. 74:3485-3489, 1977). At the nonpermissive temperature DNA synthesis in the mutant stops rapidly, the DNA is degraded to acid-soluble material, and cell death ensures. This suggests that the mutant may be among the most ligase-deficient strains yet characterized.
mesh_terms: D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D011088:DNA Ligases / Q000235:genetics* / Q000378:metabolism; D004261:DNA Replication; D004269:DNA, Bacterial / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics*; D005796:Genes; D009154:Mutation; D011118:Polynucleotide Ligases / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D011088:DNA Ligases; D011118:Polynucleotide Ligases