Identification of species of Candida, Cryptococcus, and Torulopsis by gas-liquid chromatography.
Gas-liquid chromatography was used to identify species of Candida, Cryptococcus, and Torulopsis by fatty-acid analysis of the whole-cell hydrolysate. Candida albicans characteristically revealed 2-OH C14:0 and C19:0 (chain length:number of double bonds); these were absent in other organisms. Candida curvata was characterized by a ratio of C16:1 to C16:0 of greater than 1.0. Candida guilliermondii contained C10:0, and Candida tropicalis had no C12:0, these features were used for their identification. Cryptococcus was characterized by the absence of C16:1. Torulopsis was characterized by a C16:1 to C16:0 ratio of greater than 10 accompanied by the presence of one unidentified fatty acid. These data suggested that certain Candida, Cryptococcus, and Torulopsis (the clinically important yeast-like organisms) may be identified by gas-liquid chromatography.
D002175:Candida / Q000032:analysis*; D002176:Candida albicans / Q000032:analysis; D002849:Chromatography, Gas; D003454:Cryptococcus / Q000032:analysis*; D003455:Cryptococcus neoformans / Q000032:analysis; D005227:Fatty Acids; D006801:Humans; D009181:Mycoses / Q000175:diagnosis; D009829:Oleic Acids; D010169:Palmitic Acids; D018805:Sepsis / Q000175:diagnosis
D005227:Fatty Acids; D009829:Oleic Acids; D010169:Palmitic Acids