Anti-tumor antibodies in lung cancer patients. Immunofluorescence study using various indicator cells.
By means of indirect immunofluorescence a number of primary lung cancer patient sera and control sera were tested for anti-tumor antibody activity on living tumor cells as a substrate. Antibodies against surface antigens were the most frequently detected in autologous system (in 65%) on cells derived from fresh surgical material of lung cancer. They were also found in 50% of cases using tumor cells from primary short-term culture. When established cell line of lung cancer was used (E-14) in allogeneic system, the antibodies were detected in only 22% of examined lung cancer sera. Absorption of positive sera with homogenates of normal tissues did not abolish their specific activity. Positive reactions were confined to squamous cell type of bronchogenic carcinoma.
D000912:Antibodies, Neoplasm / Q000032:analysis*; D000954:Antigens, Surface / Q000276:immunology; D002294:Carcinoma, Squamous Cell / Q000276:immunology*; D002478:Cells, Cultured; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D008175:Lung Neoplasms / Q000276:immunology*; D008297:Male; D008875:Middle Aged
D000912:Antibodies, Neoplasm; D000954:Antigens, Surface