Cloning in Escherichia coli and physical structure of hepatitis B virion DNA.
A restriction map of hepatitis B virion DNA was established after cloning of the whole viral genome in Escherichia coli. By use of EcoRI, Xho I, Bgl II, Xba I, BamHI, HincII, and Hae III endonucleases, a total of 28 restriction sites were mapped. The single-stranded region was localized on the restriction map and 5' end of the short strand was mapped at a fixed position.
D002874:Chromosome Mapping; D003090:Coliphages; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant; D004277:DNA, Single-Stranded / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics*; D004926:Escherichia coli; D006515:Hepatitis B virus / Q000235:genetics*
D004274:DNA, Recombinant; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D004262:DNA Restriction Enzymes