title: T and B lymphocytes in lymph nodes and spleen of patients with Hodgkin's disease.
abstract: Lymphocyte surface markers of 100 tissue specimens (67 lymph nodes and 33 spleens) of 40 patients with Hodgkin's disease were studied by means of spontaneous E-rosette formation and direct immunofluorescence method. The occurrence of active and total T cells was signigicantly higher (p less than 0.001) in the tumor-involved lymph nodes and spleens, while the frequency of B cells was practically the same in the histologically negative lymph nodes and spleens. In the tumorous spleen of 6 untreated patients the frequency of active and total T cells was higher in the tumorous tissue specimens than in the surrounding histologically negative tissue specimens. No correlation has been found between the simultaneously investigated peripheral T and B cell counts, clinical stage and the histological type of the tumor.
mesh_terms: D001402:B-Lymphocytes / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D006689:Hodgkin Disease / Q000276:immunology* / Q000473:pathology; D006801:Humans; D008198:Lymph Nodes / Q000276:immunology* / Q000473:pathology; D012397:Rosette Formation; D013154:Spleen / Q000276:immunology* / Q000473:pathology; D013601:T-Lymphocytes / Q000276:immunology*