Penicillin selection of Escherichia coli deoxyribonucleic acid repair mutants.
A rapid method has been developed for isolation of ultraviolet-sensitive mutants of Escherichia coli, by inducing delay in the growth and/or division of repair-deficienct cells with low fluences of far-ultraviolet radiation, and killing with penicillin the repair-proficient cells, which continue to grow and divide. With this technique, we have achieved about a 3,000-fold enrichment for photoreactivation less (phr) cells and have isolated and characterized three phr mutants.
D004260:DNA Repair; D004269:DNA, Bacterial / Q000235:genetics / Q000528:radiation effects; D004926:Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000528:radiation effects; D008027:Light; D009154:Mutation; D010406:Penicillins / Q000494:pharmacology*; D014466:Ultraviolet Rays
D004269:DNA, Bacterial; D010406:Penicillins