Selection of a low antigenic variant subline from the TA3St ascites carcinoma by repeated passage of antibody-coated cells through anti-immunoglobulin columns.
TA3St mouse ascites carcinoma cells of strain A origin (H-2a) were coated with anti-H-2a serum and passaged through mouse immunoglobulin-anti-immunoglobulin columns repeatedly prior to each in vivo passage. After six passages in vivo, a variant cell line emerged with significantly decreased H-2a expression and an increased ability to grow progressively across H-2 allograft barriers. Chromosome analysis of the variant cell line revealed no major changes in karyotype. The application of the method for studies on the generation of immunoresistant tumor cell variants and on the relationship between antibody-binding sites and rejection target sites is discussed.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D002460:Cell Line; D002469:Cell Separation / Q000379:methods*; D002869:Chromosome Aberrations; D006183:H-2 Antigens / Q000276:immunology*; D007136:Immunoglobulins / Q000276:immunology; D007163:Immunosorbent Techniques; D007518:Isoantibodies / Q000008:administration & dosage; D051379:Mice; D008815:Mice, Inbred Strains; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000235:genetics / Q000276:immunology*; D014184:Transplantation, Homologous
D000888:Antibodies, Anti-Idiotypic; D006183:H-2 Antigens; D007136:Immunoglobulins; D007518:Isoantibodies