A micro method for enumerating T and B cells in mouse peripheral blood.
A micro method has been devised to assess T and B cells in mouse peripheral blood. This technique permits simultaneous determination of B lymphocytes and monocytes. T lymphocytes are determined by a cytotoxicity assay. Tests require only 0.2 ml of whole blood and avoid the loss of certain cell populations secondary to flotation separation techniques. Percentages of IgM and IgG bearing B lymphocytes, Thy-1 bearing T lymphocytes as well as monocytes in mouse peripheral blood were determined in a total of 50 mice of 11 different strains.
D000818:Animals; D000961:Antilymphocyte Serum / Q000494:pharmacology; D001402:B-Lymphocytes; D001772:Blood Cell Count / Q000379:methods; D005260:Female; D005455:Fluorescent Antibody Technique; D008198:Lymph Nodes / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D008813:Mice, Inbred ICR; D009000:Monocytes; D011947:Receptors, Antigen, B-Cell; D013154:Spleen / Q000276:immunology; D013601:T-Lymphocytes
D000961:Antilymphocyte Serum; D011947:Receptors, Antigen, B-Cell