Restoration of depressed antibody responses of leukemic splenocytes treated with LPS-induced factors.
Lipopolysaccharide and a nontoxic derivative, PS, from Serratia marcescens were studied in terms of their effects on normal and Friend leukemia virus depressed splenocytes. These materials caused a marked increase in the number of antibody producing cells by both groups of splenocytes. MDP, a synthetic analogue of myobacterial cell walls, produced a similar adjuvant response by normal and leukemic splenocytes. Combinations of PS or LPS and BCG or MDP resulted in synergistic immunostimulatory responses. A soluble factor associated with these materials appeared to mediate the response, since cell-free supernatants from LPS stimulated splenocytes or serum from LPS treated mice resulted in similar immunostimulation.
D000119:Acetylmuramyl-Alanyl-Isoglutamine / Q000494:pharmacology; D000818:Animals; D000917:Antibody Formation; D004306:Dose-Response Relationship, Immunologic; D005622:Friend murine leukemia virus / Q000276:immunology; D007165:Immunosuppression Therapy; D007942:Leukemia, Experimental / Q000276:immunology*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051379:Mice; D009161:Mycobacterium / Q000276:immunology; D009163:Mycobacterium bovis / Q000276:immunology; D011135:Polysaccharides, Bacterial / Q000494:pharmacology; D013154:Spleen / Q000276:immunology*
D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D000119:Acetylmuramyl-Alanyl-Isoglutamine