Rapid identification of infectious pancreatic necrosis virus in infected cell cultures by immunoperoxidase techniques.
Direct and indirect immunoperoxidase (IP) techniques were evaluated for their potential in identifying infectious pancreatic necrosis (IPN) virus. Both techniques were shown to offer a relatively simple, rapid and efficient means for the specific identification of IPN virus in infected cells. The direct IP method resulted in less nonspecific staining; however, the indirect method was clearly specific and utilized commercially available reagents.
D002478:Cells, Cultured; D007124:Immunoenzyme Techniques; D012087:Reoviridae / Q000254:growth & development / Q000276:immunology*