Arrest of cell membrane movements by in vitro incubation with polycation reversed by polyanion.
A total arrest of cell membrane movement occurred after incubation for 2 h of cultured fibroblasts with the polycation DEAE-dextran. The immobilized cells resumed normal membrane undulations after exposure for 5 min to the polyanion dextran-sulphate. The influence of DEAE-dextran on the electrophoretic mobility, which was seen after 10 min of incubation was also erased by a subsequent incubation with dextran-sulphate. No influence on growth of the culture was seen.
D000818:Animals; D002452:Cell Count; D002462:Cell Membrane / Q000187:drug effects / Q000502:physiology; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D003637:DEAE-Dextran / Q000494:pharmacology; D003911:Dextrans / Q000494:pharmacology*; D004586:Electrophoresis; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology*; D051379:Mice; D008858:Microscopy, Phase-Contrast
D003911:Dextrans; D003637:DEAE-Dextran