Effect of fixation on the antigenicity of human lactoferrin in paraffin-embedded tissues and cytocentrifuged cell smears.
Immunoperoxidase techniques were used to study the preservation of the antigenicity of human lactoferrin (LF) of polymorphonuclear neutrophils (PMN) and various exocrine glandular cells in paraffin-embedded tissue blocks and cytocentrifuged cell smears. Tissues fixed in Carnoy's fluid in contrast to other fixatives used, showed good preservation of LF antigenicity irrespective of the fixation time. Cell smears fixed in Carnoy's fluid showed diffuse nuclear and cytoplasmic staining for LF, although morphologic integrity was poorly preserved. Granular cytoplasmic staining for LF with no staining of nuclei was seen in cell smears fixed in buffered formol acetone for 2--10 min. The nature of nuclear LF staining and future applications of the present methods are discussed.
D000941:Antigens; D005404:Fixatives / Q000494:pharmacology*; D006801:Humans; D007158:Immunologic Techniques; D007781:Lactoferrin / Q000097:blood / Q000276:immunology*; D007782:Lactoglobulins / Q000276:immunology*; D009504:Neutrophils / Q000166:cytology* / Q000187:drug effects
D000941:Antigens; D005404:Fixatives; D007782:Lactoglobulins; D007781:Lactoferrin