Cytolytic action of 60-F derived from live hemolytic streptococci against Ehrlich carcinoma cells.
The effect of 60-F, a fraction obtained by 0.5 approximately 0.6 saturation of ammonium sulfate of streptomycin-pretreated cell-free extract from live hemolytic streptococci (avirulent Su strain), on release of 51Cr from the 51Cr-labeled Ehrlich ascites carcinoma cells was studied with following results: a) 60-F was found to be highly effective in releasing 51Cr from 51Cr-labeled Ehrlich ascites carcinoma cells. b) Additionally, destructive picutres of the tumor cells contacted with 60-F in vitro was observed by phase-contrast microscopic examination. c) Indication was that the 51Cr-releasing assay method is also useful for the study of the direct cytolytic effect of 60-F.
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001688:Biological Products / Q000494:pharmacology*; D002286:Carcinoma, Ehrlich Tumor / Q000648:ultrastructure*; D002470:Cell Survival / Q000187:drug effects; D002860:Chromium Radioisotopes; D007553:Isotope Labeling; D051379:Mice; D008858:Microscopy, Phase-Contrast; D013291:Streptococcus / Q000032:analysis*
D000970:Antineoplastic Agents; D001688:Biological Products; D002860:Chromium Radioisotopes