A new method for the preparation of mammalian spermatogonial chromosomes.
After the seminiferous tubules were minced and washed with phosphate-buffered saline to remove sperms, spermatids and spermatocytes (Hoo and Bowles, 1971), they were repeatedly treated with 0.1% trypsin solution to liberate spermatogonia. It was concluded that the spermatogonial chromosomes can be analysed much more easily and accurately by this procedure than by previous ones.
D000818:Animals; D002875:Chromosomes; D003584:Cytological Techniques; D007621:Karyotyping; D008297:Male; D051379:Mice; D051381:Rats; D013093:Spermatogonia / Q000648:ultrastructure*; D013094:Spermatozoa / Q000648:ultrastructure*; D014357:Trypsin
D014357:Trypsin