Plasmodium falciparum in culture: improved continuous flow method.
A new design of flow vessel provides a method for continuous culture of P. falciparum in a settled layer of human erythrocytes with a slow flow of culture medium over them. The parasitemia is kept fluctuating from approximately 1%, just after addition of fresh erythrocytes, to approximately 10%, 2 or 3 days later. Each vessel provides each week 3 harvests, each containing approximately 0.6--1 X 10(9) parasites.
D000818:Animals; D002455:Cell Division; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods*; D004912:Erythrocytes / Q000469:parasitology; D005947:Glucose / Q000378:metabolism; D006801:Humans; D010963:Plasmodium falciparum / Q000254:growth & development* / Q000378:metabolism
D003470:Culture Media; D005947:Glucose