title: [Cell aging in vitro].
abstract: The phenomenon of in vitro cell ageing has been considered in terms of limited multiplication and defined life span of cultured diploid cell strains and lines in vitro and in situ. Theories on cell ageing are discussed. Original recent evidence is provided concerning mouse embryo fibroblast ageing in vitro. Phenotypes of early, middle and late passage cells, observed with transmission and scanning electron microscopes, are described. Using light microscope autoradiography, proliferative capacity of these cells has been examined that involves replicative index, cell cycle durations, and 3H-actinomycin binding index. Transcription peculiarities of cultured cells of different age are described using such parameters as activities of RNA polymerases and of RNA synthesis estimated biochemically in isolated nuclei, and with the help of light autoradiography in living cells.
mesh_terms: D000375:Aging; D000818:Animals; D002453:Cell Cycle; D002455:Cell Division; D002460:Cell Line; D002470:Cell Survival; D002478:Cells, Cultured / Q000502:physiology*; D005347:Fibroblasts / Q000502:physiology / Q000648:ultrastructure; D006801:Humans; D066298:In Vitro Techniques; D008938:Mitosis; D008967:Molecular Biology; D010641:Phenotype; D012313:RNA / Q000096:biosynthesis; D014158:Transcription, Genetic
chemical_list: D012313:RNA
vernacular_title: Starenie kletok in vitro.