Restriction map of the region surrounding the EcoRI site in the pCR1 plasmid and analysis of an inserted ovalbumin gene.
We have determined a restriction map of a 1650 base pair region surrounding the EcoRI site of the bacterial plasmid, pCR1. We have used pCR1 as a vector in cloning synthetic ovalbumin double-stranded cDNA. Using the pCR1 restriction map, we have characterized the ovalbumin sequences inserted in one recombinant plasmid, pOvE12. POvE12 appears to contain all, or nearly all, of the sequences found in full length, double-stranded cDNA synthesized in vitro.
D001483:Base Sequence; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D004262:DNA Restriction Enzymes / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D004274:DNA, Recombinant; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D005796:Genes; D007612:Kanamycin / Q000494:pharmacology; D010047:Ovalbumin / Q000235:genetics*; D010957:Plasmids; D014166:Transferases / Q000235:genetics; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; D004274:DNA, Recombinant; D007612:Kanamycin; D010047:Ovalbumin; D014166:Transferases; D004262:DNA Restriction Enzymes