Plate diffusion assay as a rapid method for dosimetry of mutagens.
This paper presents a method for determining mutagenic concentrations of chemicals by using an agar diffusion assay. The method is based on the linear relationship between the amount of chemical placed at the center of the dish and the radius of the mutagenic zone. A brief theoretical discussion and experimental data confirming this relationship are given. Alkylating agents and mycotoxins were used to test the system. This method can be used to follow up decreased mutagenic potencies of solutions of unstable mutagens and to follow the production of mutagenic substances throughout fermentation.
D001681:Biological Assay / Q000379:methods*; D004305:Dose-Response Relationship, Drug; D005020:Ethyl Methanesulfonate / Q000494:pharmacology; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D008770:Methylnitrosourea / Q000494:pharmacology; D009153:Mutagens / Q000032:analysis*; D009154:Mutation; D009183:Mycotoxins / Q000494:pharmacology; D009242:N-Nitrosopyrrolidine / Q000494:pharmacology; D009602:Nitrosamines / Q000494:pharmacology; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics
D009153:Mutagens; D009183:Mycotoxins; D009602:Nitrosamines; D008769:Methylnitronitrosoguanidine; D008770:Methylnitrosourea; D005020:Ethyl Methanesulfonate; D009242:N-Nitrosopyrrolidine