Identification of immunoreactive sites in bovine parathyroid cells to antibodies raised against the NH2-terminal sequence of parathyroid hormone.
The NH2-terminal sequence of bovine parathyroid hormone (1-84) was localized with different immunocytochemical methods on the light and electron microscopic level in bovine parathyroid glands and in isolated bovine parathyroid parenchymal cells. The peroxidase labeled staphylococcal protein A and the peroxidase anti-peroxidase method were found to be advantageous for light and electron microscopic localization, respectively. Reaction product was found light microscopically in the cytoplasma of the parenchymal cells and electron microscopically largely over the secretion granules of the parenchymal cells. The immunoreactive sites were subsequently identified to represent only intact parathyroid hormone (1-84) by gel electrophoresis derived enzyme linked immunosorbent assay.
D000818:Animals; D000906:Antibodies; D002417:Cattle; D006651:Histocytochemistry; D007158:Immunologic Techniques; D007163:Immunosorbent Techniques; D010280:Parathyroid Glands / Q000032:analysis*; D010281:Parathyroid Hormone / Q000032:analysis* / Q000276:immunology; D010446:Peptide Fragments / Q000276:immunology
D000906:Antibodies; D010281:Parathyroid Hormone; D010446:Peptide Fragments