[Use of the passive hemagglutination inhibition reaction for the purpose of determining the antigenic activity of anatoxins in vitro].
The work deals with the study of the possibility of using the passive hemagglutination inhibition test (antibody neutralization) for the determination of the antigenic activity of botulinum toxoids, types A, B and E. Erythrocytic diagnostic preparations were shown to allow the determination of up to 0.1 g of antigen. At the same time in vitro determinations were found to satisfactorily correlate with the results of in vivo determinations of the antigenic activity of these toxoids in the antitoxin-fixation test.
D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis*; D000992:Antitoxins / Q000276:immunology; D001666:Binding Sites, Antibody; D003014:Clostridium botulinum / Q000276:immunology; D003017:Clostridium tetani / Q000276:immunology; D004168:Diphtheria Toxoid / Q000276:immunology; D004912:Erythrocytes / Q000276:immunology; D006385:Hemagglutination Inhibition Tests; D066298:In Vitro Techniques; D008297:Male; D051379:Mice; D012756:Sheep / Q000276:immunology; D014121:Toxoids / Q000276:immunology*
D000942:Antigens, Bacterial; D000992:Antitoxins; D004168:Diphtheria Toxoid; D014121:Toxoids
Ispol'zovanie reaktsii tormozheniia passivnoĭ gemaggliutinatsii s tsel'iu opredeleniia antigennoĭ aktivnosti anatoksinov in vitro.