Binding of lac repressor to the secondary lac operator in Escherichia coli.
In the lac operon, the existence of a secondary repressor binding site, inside Z gene, had been inferred from in vitro binding studies (Reznikoff et al., 1974; Gilbert et al., 1975). A series of deletions have been constructed from a lac transducing lambda bacteriophage. Some of those deleted bacteriophages have still the property of derepressing a chromosomal lac operon, even though they do not contain any more the lac operator. This phenomenon is an indication that the secondary repressor binding site is also active in vivo.
D001665:Binding Sites; D002872:Chromosome Deletion; D002876:Chromosomes, Bacterial; D004926:Escherichia coli / Q000235:genetics*; D005809:Genes, Regulator; D007763:Lac Operon; D012097:Repressor Proteins / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*; D014161:Transduction, Genetic
D012097:Repressor Proteins; D014157:Transcription Factors