[Comparison of different methods for measuring nucleic acids and protein in the biomass, half-products and preparations of E. coli and yeast].
In the E. coli biomass, half-products and RNA preparations a comparative measurement of nucleic acids and protein was carried out using different methods. As a result, the following methods can be recommended: to assay RNA in the biomass--spectrophotometry, to determine RNA in half-products and preparations--the phosphate method, to assess DNA--the diphenyl amine method, and to measure protein--according to Lowry. The same methods can be recommended to estimate RNA from baker's yeast.
D001426:Bacterial Proteins / Q000032:analysis*; D003124:Colorimetry / Q000379:methods; D004269:DNA, Bacterial / Q000032:analysis*; D004271:DNA, Fungal / Q000032:analysis*; D004926:Escherichia coli / Q000032:analysis*; D005656:Fungal Proteins / Q000032:analysis*; D012329:RNA, Bacterial / Q000032:analysis*; D012331:RNA, Fungal / Q000032:analysis*; D012441:Saccharomyces cerevisiae / Q000032:analysis*; D013053:Spectrophotometry / Q000379:methods
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004271:DNA, Fungal; D005656:Fungal Proteins; D012329:RNA, Bacterial; D012331:RNA, Fungal
Sravnenie razlichnykh metodov opredeleniia nukleinovykh kislot i belka v biomasse, poluproduktakh i preparatakh E. Coli i drozhzheĭ.