Primary structure of yeast proteinase B inhibitor 2.
The complete amino acid sequence of yeast proteinase B inhibitor 2 (IB2) was determined to be H3N+-Thr-Lys-Asn-Phe-Ile-Val-Thr-Leu-Lys-Lys-Asn-Thr-Pro-Asp-Val-Glu-Ala-Lys-Lys-Phe-Leu-Asp-Ser-Val-His-His-Ala-Gly-Gly-Ser-Ile-Leu-His-Glu-Phe-Asp-Ile-Ile-Lys-Gly-Tyr-Thr-Ile-Lys-Val-Pro-Asp-Val-Leu-His-Leu-Asn-Lys-Leu-Lys-Glu-Lys-His-Asn-Asp-Val-Ile-Glu-Asn-Val-Glu-Asp-Lys-Glu-Val-His-Thr-Asn-COO-. Elucidation of the primary structure was enabled by automated Edman degradation and COOH-terminal hydrolysis with carboxypeptidases A (bovine pancreas and Y (yeast). IB2 is the first proteinase inhibitor to be sequenced that possesses a structure devoid of disulfide bridges.
D000595:Amino Acid Sequence; D000818:Animals; D002268:Carboxypeptidases; D002417:Cattle; D006023:Glycoproteins / Q000032:analysis*; D010179:Pancreas / Q000201:enzymology; D010446:Peptide Fragments / Q000032:analysis; D011480:Protease Inhibitors; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000201:enzymology; D014357:Trypsin
D006023:Glycoproteins; D010446:Peptide Fragments; D011480:Protease Inhibitors; C018169:yeast proteinase B inhibitor; D002268:Carboxypeptidases; D014357:Trypsin