A modified mixed haemadsorption procedure for the detection and localisation of sperm antigens.
A mixed haemadsorption procedure for the detection and localisation of spermatozoal antigens is described. Sperm are immobilised by attaching them to the bases of wells on plastic plates using poly-L-lysine and antisera and sensitised red cells are then added to these wells. The technique uses simple reagents and allows sperm/red cell rosettes to be kept as permanent records.
D000818:Animals; D000941:Antigens; D000961:Antilymphocyte Serum / Q000494:pharmacology; D006382:Hemadsorption; D007158:Immunologic Techniques; D008297:Male; D051379:Mice; D008805:Mice, Inbred A; D013094:Spermatozoa / Q000276:immunology*
D000941:Antigens; D000961:Antilymphocyte Serum