[Immunofluorescence method of determining HBc-antibodies in the blood sera of serum hepatitis type B patients].
The indirect immunofluorescent procedure was used to examine blood sera from 68 patients with serum hepatitis having HBsAg. HBcAb were found in 85% of the specimens. Formalin-fixed paraffin sections of autopsy livers pretreated with enzyme and containing HBcAg in hepatocyte nuclei were shown to be a useful tissue substrate. HBcAb were found in patients in a wave-like manner. The presence of HBcAb was found to be connected with the active phase of serum hepatitis.
D000293:Adolescent; D000328:Adult; D000368:Aged; D000914:Antibodies, Viral / Q000032:analysis*; D003378:Counterimmunoelectrophoresis; D005455:Fluorescent Antibody Technique; D006509:Hepatitis B / Q000276:immunology*; D006510:Hepatitis B Antibodies / Q000032:analysis*; D006514:Hepatitis B Surface Antigens / Q000032:analysis; D006801:Humans; D008875:Middle Aged; D011233:Precipitin Tests; D011863:Radioimmunoassay
D000914:Antibodies, Viral; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens
Opredelenie metodom immunofliuorestsentsii HBc-antitel v syvorotkakh krovi bol'nykh syvorotochnym gepatitom tipa B.