title: Isolation and characterization of yeast mutants auxotrophic for 2'-deoxythymidine 5'-monophosphate.
abstract: Mutant strains of Saccharomyces cerevisiae auxotrophic for deoxythymidine monophosphate (dTMP) were isolated and characterized. Two distinct classes of auxotrophs were obtained. One class had a simple requirement for dTMP and was analogous to thymine-requiring bacteria. The second class required dTMP, adenine, histidine and methionine and this complex nutritional phenotype was due to defects in folate metabolism. The dTMP-dependent growth of respiratory-competent grande auxotrophs was found to be markedly affected by media composition and carbon source. In the absence of dTMP thymineless death occurred in both mutant classes.
mesh_terms: D004261:DNA Replication; D005492:Folic Acid / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013938:Thymidine Monophosphate / Q000235:genetics* / Q000378:metabolism; D013942:Thymine Nucleotides / Q000235:genetics*
chemical_list: D013942:Thymine Nucleotides; D013938:Thymidine Monophosphate; D005492:Folic Acid