Asialoprotein uptake by liver cells: immunofluorescence microscopy.
Uptake of asialoproteins by hepatocytes causes a change in the intracellular pattern of immunofluorescence. Control cells display a peripheral fluorescence which probably represents nascent proteins. Dark nonfluorescent areas, that presumably contain glycogen, are located around the nucleus. In contrast, liver cells from rats injected with asialoproteins display a pancytoplasmic fluorescence due to an influx of endocytotic vesicles.
D000818:Animals; D001692:Biological Transport; D001798:Blood Proteins / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006023:Glycoproteins / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D014168:Transferrin / Q000378:metabolism
D001798:Blood Proteins; D006023:Glycoproteins; D014168:Transferrin