[Kinetics of phenylacetamide hydrolysis by immobilized penicillinamidase].
The kinetics of phenylacetamide hydrolysis catalyzed by polyacrylamide gel-immobilized penicillinamidase were studied. The Km and Kp values obtained were compared to the literary data for the specific substrate--benzylpenicillin. It was shown that the type of inhibition by the reaction product was the same, whereas the efficiency of binding of phenylacetic acid depended on the substrate structure.
D000581:Amidohydrolases / Q000378:metabolism*; D004800:Enzymes, Immobilized / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology; D007700:Kinetics; D010399:Penicillin Amidase / Q000378:metabolism*; D013379:Substrate Specificity
D004800:Enzymes, Immobilized; D000581:Amidohydrolases; D010399:Penicillin Amidase
Kinetika gidroliza fenilatsetamida immobilizovannoĭ penitsillinamidazoĭ.