[Immunofluorescence method of study of biosynthesis of human interferon].
The method of fluorescent antibody (MFA) has first been used for the study of regularities of human interferon biosynthesis in superinduced cells. The dynamics of the appearance and accumulation of interferon in individual cells and the dependence of these processes upon the time elapsing since the termination of superinduction were determined. Clear-cut inverse relationship between the interferon content in cells (MFA) and the interferon content in the culture fluid (titrations of biological activity) was established.
D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000166:cytology; D004666:Encephalitis Virus, Venezuelan Equine; D005260:Female; D005347:Fibroblasts / Q000378:metabolism / Q000382:microbiology; D005455:Fluorescent Antibody Technique; D006801:Humans; D007369:Interferon Inducers; D007372:Interferons / Q000096:biosynthesis*; D007668:Kidney / Q000166:cytology; D011247:Pregnancy; D012867:Skin / Q000166:cytology
D007369:Interferon Inducers; D007372:Interferons
Immunofliuorestsentnyĭ analiz biosinteza chelovecheskogo interferona.