[Blocking action of the soluble H-2 antigens and H-2 antibody isolated by various methods and mouse immune lymphocytes].
The treatment of tumour and lymphoid cells of mice with 3M KC1 solution having high ionic strength, nonionic detergent and by subsequent freeze-thawing resulted in obtaining serologically active H-2 antigen preparation capable of specifically blocking the cytotoxicity of H-2 antisera. The antigenic activity of the preparations thus obtained depended on the source from which they were isolated (spleen cells and their membrane fragments proved to be the best source), on the degree of maturity of tumor cells and the degree of purification of the preparation, as well as on the methods of solubilization. The blocking action of soluble H-2 antigens on the cytotoxicity of immune lymphocytes depended on the method used for isslating these antigens. The interaction of immune lymphocytes and H-2 antisera with soluble antigens was probably effected by different mechanisms.
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification*; D000936:Antigen-Antibody Complex; D001667:Binding, Competitive; D006649:Histocompatibility Antigens / Q000302:isolation & purification*; D007158:Immunologic Techniques; D007939:Leukemia L1210 / Q000276:immunology; D008198:Lymph Nodes / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D012513:Sarcoma, Experimental / Q000276:immunology; D012995:Solubility; D013154:Spleen / Q000276:immunology
D000906:Antibodies; D000936:Antigen-Antibody Complex; D006649:Histocompatibility Antigens
Blokiruiushchee deiĭstvie vydelennykh razlichnymi metodami rastvorimykh antigenov H-2 i H-2-antitela i immunnye limfotsity mysheĭ.