Biochemical and topographical studies on Escherichia coli cell surface. IV. Giant spheroplast formation from a filamentous cell.
Long, nonseptate filamentous cells consisting of 5 to 40 single-cell unit lengths were formed from Escherichia coli surface mutant ONT-3 by treatment with a sublethal concentration of sodium dodecyl sylfate. As distinct from several other elongated cells (e.g., thymine-starved filaments), it was found here that stable giant spheroplasts, 5 to 10 micrometers in diameter, were produced by the action of lysozyme in the presence of bovine serum albumin via the gradual fusion of distinct spheroplasting bulbs.
D002473:Cell Wall / Q000378:metabolism; D004926:Escherichia coli / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D009113:Muramidase / Q000378:metabolism; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013104:Spheroplasts / Q000302:isolation & purification* / Q000648:ultrastructure
D012967:Sodium Dodecyl Sulfate; D009113:Muramidase