Mutagenic effects of benzo[a]pyrene after metabolic activation by hepatic 9000 g supernatants or intact hepatocytes.
The activating capacities of isolated rat hepatocytes and 9000 g supernatant from these cells with respect to the mutagenic effect of benzo[a]pyrene on Salmonella typhimurium TA100 were investigated. No mutagenicity of benzo[a]pyrene was found with the cell-mediated assay, unless the hepatocytes were disrupted after pre-incubation with benzo[a]pyrene or the intracellular glutathione content was reduced. It is suggested that a retention of active metabolites and an effective detoxication may account for the absence of mutagenic response.
D001580:Benzopyrenes / Q000494:pharmacology*; D001711:Biotransformation; D002458:Cell Fractionation; D005821:Genetic Techniques; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D009153:Mutagens; D012486:Salmonella typhimurium / Q000235:genetics
D001580:Benzopyrenes; D009153:Mutagens