Comparative susceptibility of starch granules of double- and triple-mutants containing amylose-extender, waxy, sugary-1, sugary-2 and dull genes of maize inbred OH43 (Zea mays L.) to amylase.
Starch granules were prepared from 14 double- and 26 triple-mutants containing amylose-extender (ae), 14 double- and 18 triple-mutants containing waxy (wx), 15 double- and 20 triple-mutants containing sugary-1 (su1), 13 double- and 23 triple-mutants containing sugary-2 (su2), and 14 double- and 19 triple-mutants containing dull (du) of maize inbred Oh43 (Zea mays L.). The relative susceptibilities of these starch granules to fungal glucoamylase were determined and the starch granules were examined by scanning electron microscopy. A commercial normal maize starch was used as a control. Starch granules of the double- and triple-mutants containing su1 and su2 were digested two to eight times faster than normal. The ae gene reduced susceptibility and seems to be epistatic to su1 and su2. Starch granules of the double- and triple-mutants containing wx were digested about two times faster than normal and those containing shrunken-2 (sh2) were digested 1.2 to eight times faster than normal. Starch granules of triple-mutant combinations with opaque-2 (o2) showed digestion properties which were comparable to those of their respective monopaque double-mutant counterpart.
D005087:Glucan 1,4-alpha-Glucosidase / Q000378:metabolism*; D005949:Glucose Oxidase / Q000378:metabolism; D005959:Glucosidases / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D009154:Mutation; D010544:Peroxidases / Q000378:metabolism; D010944:Plants / Q000235:genetics*; D013045:Species Specificity; D013213:Starch; D003313:Zea mays / Q000235:genetics
D013213:Starch; D005949:Glucose Oxidase; D010544:Peroxidases; D005959:Glucosidases; D005087:Glucan 1,4-alpha-Glucosidase