A simple method for the detection of antibodies to Encephalitozoon cuniculi in rabbits.
Rabbit antibodies against Encephalitozoon cuniculi were detected in an indirect microagglutination test using a bead substrate to which anti-rabbit immunoglobin G light and heavy chain antibodies were coupled. The test was positive using immune whole serum or F(ab)' and F(ab)'2 fragments of immunoglobin G but negative using the F(c) fragment. The reaction was blocked by saturating the beads with rabbit serum or by absorbing positive sera with excess Encephalitozoon cuniculi. The test provided a simple method to detect antibodies to Encephalitozoon cuniculi, did not require elaborate equipment and could be performed using frozen antigen.
D000372:Agglutination Tests; D000818:Animals; D000906:Antibodies / Q000032:analysis*; D056890:Eukaryota / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D007140:Immunoglobulin Fab Fragments / Q000032:analysis; D007141:Immunoglobulin Fc Fragments / Q000032:analysis; D011528:Protozoan Infections / Q000175:diagnosis; D011529:Protozoan Infections, Animal; D011817:Rabbits / Q000276:immunology*
D000906:Antibodies; D007140:Immunoglobulin Fab Fragments; D007141:Immunoglobulin Fc Fragments