title: Preparation of immune sera against carcinoembryonic antigen (CEA).
abstract: CEA antigen obtained by the Wrocław team and CEA standard antigen from Chester Beatty Cancer Research Institute, London were used for immunization of goats. Two immunization methods were adopted: one with decreasing antigen doses beginning from 500 microgram and the other with equal, small doses of 10 microgram. Anti-goat IgG1 + IgG2 immune serum was obtained by immunizing a horse with 10 mg of the antigen with complete Freund's adjuvant. Anti-CEA immune sera obtained after immunization with small doses were highly active; in the RIA test they showed half maximum CEA binding at 1:40,000 and 1:80,000 dilutions. Likewise, the anti IgG1 and IgG2 serum from the horse proved to be highly active.
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D002272:Carcinoembryonic Antigen; D006041:Goats; D006801:Humans; D007106:Immune Sera; D007115:Immunization Schedule; D007158:Immunologic Techniques
chemical_list: D002272:Carcinoembryonic Antigen; D007106:Immune Sera