Isolation and characterization of recombinant clones containing the chicken adult beta-globin gene.
We have isolated and characterized two independent clones containing the chicken adult beta-globin gene. Each clone contains a 6.2-kilobase-pair Eco RI restriction fragment of chicken erythrocyte DNA inserted into the vector, lambda gtWES . lambda B. The orientation of the inserted fragment is opposite in the two clones. Characterization of the clones by electron microscopic R-loop studies, by restriction enzyme mapping, and by filter hybridization shows that the adult beta-globin gene is interrupted by at least one small and one large intervening sequence. In addition to the complete adult beta-globin gene, at least part of a second beta-globin-like gene was identified about 2.7 kilobase pairs from the 3'-end of the adult gene. The two independent clones, while very similar, do differ at two Msp I restriction endonuclease sites in regions flanking the adult beta-globin gene.
D000818:Animals; D002645:Chickens; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D005914:Globins / Q000096:biosynthesis*; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D014176:Protein Biosynthesis; D014158:Transcription, Genetic
D004274:DNA, Recombinant; D005914:Globins; D004262:DNA Restriction Enzymes