title: [Synthesis of globin mRNA in cultured murine erythroleukemic cells, transformed by Rauscher leukemia virus].
abstract: In contrast to cultured erythroleukemic cells transformed by Friend leukemia virus, in the cultured erythroleukemic murine cells transformed by Rauscher leukemia virus, the synthesis of haemoglobin cannot be induced after treatment with 1--2% dimethylsulfoxide, actinomycin D and proteolytic enzymes. Study of disturbances in the haemoglobin synthesis in this cell culture revealed that globin 9S-mRNA is either absent or present in negligible amounts in the culture. The data obtained suggest that the disturbances in haemoglobin synthesis occur at the level of transcription or processing of globin mRNA, rather than at the level of translation.
mesh_terms: D000818:Animals; D002460:Cell Line; D002472:Cell Transformation, Viral; D003609:Dactinomycin / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005914:Globins / Q000096:biosynthesis*; D004915:Leukemia, Erythroblastic, Acute; D051379:Mice; D010447:Peptide Hydrolases / Q000494:pharmacology; D014176:Protein Biosynthesis / Q000187:drug effects; D012333:RNA, Messenger / Q000096:biosynthesis*; D011925:Rauscher Virus; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D012333:RNA, Messenger; D003609:Dactinomycin; D005914:Globins; D010447:Peptide Hydrolases; D004121:Dimethyl Sulfoxide
vernacular_title: Narushenie sinteza mRNK globina v kul'ture eritroblastov myshi, transformirovannykh virusom raushera.