2'-Deoxy-2'-fluorouridine-5'-triphosphates: a possible substrate for E. coli RNA polymerase.
dUflTP was tested as substrate in the E. coli RNA polymerase system using poly(dAT) as template. dUflTP could replace UTP when Mn++ was utilized as divalent cation instead of Mg++. The level of transcription with the fluoro analog was then 55% of that with UTP.
D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D003856:Deoxyuracil Nucleotides; D004926:Escherichia coli / Q000201:enzymology*; D007700:Kinetics; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D013379:Substrate Specificity; D014158:Transcription, Genetic / Q000187:drug effects; D014544:Uridine Triphosphate
D003856:Deoxyuracil Nucleotides; D008345:Manganese; C019324:2'-deoxy-2'-fluorouridine-5'-triphosphate; D012321:DNA-Directed RNA Polymerases; D008274:Magnesium; D014544:Uridine Triphosphate