Genotypic characterization and clinical evaluation of an influenza A/Texas/1/77 (H3N2)-like recombinant: RIT 4199.
The RIT 4199 (H3N2) strain was obtained by recombination of PR/8/34 (H0N1) and A/Alaska/5/77 (H3N2) isolates. Its genetic composition was determined by RNA-RNA hybridization and by identification by gel electrophoresis of the double-standard RNA formed. The RIT 4199 was inoculated intranasally to 27 seronegative volunteers at a dose of 10(7.3)EID50. The results show that it is suitable for live vaccine strain.
D000328:Adult; D002986:Clinical Trials as Topic; D005814:Genes, Viral; D005838:Genotype; D006801:Humans; D053122:Influenza A Virus, H3N2 Subtype; D009980:Influenza A virus / Q000235:genetics*; D007252:Influenza Vaccines / Q000302:isolation & purification / Q000627:therapeutic use*; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D012367:RNA, Viral / Q000032:analysis / Q000096:biosynthesis; D011995:Recombination, Genetic; D013997:Time Factors; D014611:Vaccination
D007252:Influenza Vaccines; D012367:RNA, Viral