title: Immunoprecipitation of biosynthetically-labelled products in the identification of antigens of murine red cells infected with the protozoan parasite, Plasmodium berghei.
abstract: In this methodological paper an immunoprecipitation technique has been optimised for the identification of antigens of Plasmodium berghei-infected blood which react with antibody specificities in a host-protective antiserum. Extracted 3H-leucine biosynthetically-labelled products of infected blood were sequentially reacted and precipitated with sera from mice which had been exposed to P. berghei but which were either non-protected or protected against lethal infection, protection having been shown to be transferable to naive recipients with the appropriate serum. As analysed by polyacrylamide gel electrophoresis under reducing conditions, a small number of molecular species was detected in immunoprecipitates using host-protective sera which were apparently not quantitatively precipitated out of the complex mixture of labelled products of infected blood using sera from non-protected mice.
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D000941:Antigens / Q000032:analysis*; D011232:Chemical Precipitation; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000469:parasitology*; D005260:Female; D007158:Immunologic Techniques; D007700:Kinetics; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D010962:Plasmodium berghei / Q000276:immunology*; D011868:Radioisotopes; D012156:Reticulocytes / Q000378:metabolism / Q000469:parasitology
chemical_list: D000941:Antigens; D011868:Radioisotopes