Analysis of the venom of the Sydney funnel-web spider, Atrax robustus using gas chromatography mass spectrometry.
Thirteen compounds have been identified using gas chromatography mass spectrometry in the venom of the Sydney funnel-web spider, Atrax robustus. The compounds were identified as their trimethylsilyl or pentafluoropropionate derivatives and were citric acid, lactic acid, phosphoric acid, glycerol, urea, glucose, gamma-aminobutyric acid, glycine, spermidine, spermine, tyramine and octopamine. Female venom contained trace quantities of 5-methyoxytryptamine which was not detected in male venom. Quantitative determination of tyramine and octopamine was achieved using chemical ionization (CH4) gas chromatography mass spectrometry and deuterated internal standards.
D001180:Arthropod Venoms / Q000032:analysis*; D002849:Chromatography, Gas / Q000379:methods; D004583:Electrons; D007477:Ions; D013058:Mass Spectrometry / Q000379:methods; D009655:Octopamine / Q000032:analysis; D011422:Propionates / Q000032:analysis; D013111:Spider Venoms / Q000032:analysis*; D013112:Spiders; D014297:Trimethylsilyl Compounds / Q000032:analysis; D014439:Tyramine / Q000032:analysis
D001180:Arthropod Venoms; D007477:Ions; D011422:Propionates; D013111:Spider Venoms; D014297:Trimethylsilyl Compounds; D009655:Octopamine; D014439:Tyramine