Detection of albumin-secreting hepatocytes at the single cell level by a protein A plaque assay.
In a complement-dependent assay, using protein A-coupled erythrocytes as target cells and anti-albumin immunoglobulin (Ig) as a developing serum, it was possible to detect single albumin-secreting hepatocytes as plaque-forming cells. Plasmacytoma cells were detected using anti-Ig antibodies and did not form plaques with anti-albumin Ig. This method can potentially be used for detecting any cell-secreting products in sufficient amounts.
D000418:Albumins / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D006462:Hemolytic Plaque Technique; D008099:Liver / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D010954:Plasmacytoma / Q000276:immunology; D013205:Staphylococcal Protein A / Q000276:immunology*
D000418:Albumins; D013205:Staphylococcal Protein A