Identification and characterization of a self-regulated repressor of translocation of the Tn3 element.
Gene fusions that bring expression of the lacZ gene under control of transcriptional and trnaslational signals within the transposable element Tn3 have been used to study regulation of Tn3-specified proteins. A gene encoding a 21,355-Mr peptide that represses translocation of Tn3 and acts at the level oquenced; amber, missense, and cis-dominant (operator-constitutive) point mutations in this gene have been isolated and characterized.
D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics; D001483:Base Sequence; D004251:DNA Transposable Elements; D004926:Escherichia coli / Q000235:genetics*; D005809:Genes, Regulator; D007763:Lac Operon; D008970:Molecular Weight; D009154:Mutation; D010957:Plasmids; D012097:Repressor Proteins / Q000502:physiology*; D014157:Transcription Factors / Q000502:physiology*
D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D012097:Repressor Proteins; D014157:Transcription Factors