title: Alterations of lipid metabolism in mice injected with endotoxin.
abstract: Some alterations in lipid metabolism in mice were observed by the intraperitoneal injection of endotoxin from Salmonella typhimurium. The content of serum triglyceride increased markedly in poisoned mice 16-24 hr postintoxication. The level of free fatty acid (FFA) in the serum of endotoxin-administered mice decreased in inverse proportion to an increase in the injected dose of endotoxin. The electrophoretic analysis of the serum lipoprotein on cellulose acetate membrane showed that pre beta-lipoprotein increased markedly and that FFA fraction in the poisoned mice sera disappeared 18 hr postintoxication. The activity of hormone-sensitive lipase in adipose tissue was elevated appreciably 2 hr after injection, but decreased more significantly after 18 hr than that in fasted control mice. On the other hand, the activity of lipoprotein lipase decreased in the post-heparin serum and adipose tissue 3 hr postintoxication, and decreased significantly after 16 hr. There were no significant differences between changes in the formation of active glycerol (alpha-GP) and in the activity of alpha glycerophosphate dehydrogenase (alpha-GPDH) in the mice liver with or without administration of endotoxin, and after 16 hr levels of both hepatic alpha-GP content and alpha-GPDH activity in poisoned mice showed a tendency to be slightly lower than those in fasted control mice.
mesh_terms: D000818:Animals; D004588:Electrophoresis, Cellulose Acetate; D004731:Endotoxins / Q000008:administration & dosage*; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005994:Glycerophosphates / Q000096:biosynthesis; D007274:Injections, Intraperitoneal; D050356:Lipid Metabolism; D008071:Lipoprotein Lipase / Q000378:metabolism; D008074:Lipoproteins / Q000097:blood; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D010743:Phospholipids / Q000097:blood; D012486:Salmonella typhimurium; D014280:Triglycerides / Q000097:blood
chemical_list: D004731:Endotoxins; D005230:Fatty Acids, Nonesterified; D005994:Glycerophosphates; D008074:Lipoproteins; D010743:Phospholipids; D014280:Triglycerides; D008071:Lipoprotein Lipase