Quantitative study on anomeric forms of glucose produced by alpha-glucosidases.
Anomeric forms of glucose produced from phenyl alpha-maltoside, maltose, or phenyl alpha-glucoside have been determined quantitatively by simultaneous measurements of optical rotation and reducing power, for eight kinds of glucose-producing 1,4-alpha-glucosyl hydrolases, including glucose-forming amylase from human urine, and alpha-glucosidases from pig serum, honey bee, buckwheat seed, rice seed, sugar beet seed, flint corn seed, and brewer's yeast. All the eight enzymes studied were found to produce alpha-glucose exclusively.
D000818:Animals; D005947:Glucose; D005959:Glucosidases / Q000378:metabolism*; D006801:Humans; D008433:Mathematics; D013045:Species Specificity; D013237:Stereoisomerism; D013379:Substrate Specificity; D000520:alpha-Glucosidases / Q000378:metabolism*
D005959:Glucosidases; D000520:alpha-Glucosidases; D005947:Glucose