title: [Effect of rifampicin and RNA polymerase changing mutation on the spectrum of proteins synthesized in Escherichia coli cells].
abstract: Rifampicin (30 mkg/ml) drastically changes the spectra of proteins synthesized by E. coli cells. The formation of some polypeptides is stimulated while that of others is inhibited. Thus, the earlier reported rifampicin stimulation of the synthesis of RNA polymerase beta and beta'-polypeptides is not an exception, the formation of some other proteins is being also enhanced by the antibiotic. After infection of UV-irradiated cells by lambda rifd47 transducing phage the viral proteins are less inhibited than the bacterial ones encoded by the phage. The spectra of proteins synthesized are also affected by rpoC1 mutation at non-permissive temperature. The obtained data suggest that rifampicin and rpoC1 mutation change the interaction of RNA polymerase with different promoters and/or regulatory factors.
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism* / Q000528:radiation effects; D005838:Genotype; D008970:Molecular Weight; D009154:Mutation; D010452:Peptide Biosynthesis; D012293:Rifampin / Q000494:pharmacology*; D013045:Species Specificity; D014158:Transcription, Genetic / Q000187:drug effects*; D014161:Transduction, Genetic; D014466:Ultraviolet Rays
chemical_list: D001426:Bacterial Proteins; D012321:DNA-Directed RNA Polymerases; D012293:Rifampin
vernacular_title: Vliianie rifampitsina i mutatsii, izmeniaiushcheĭ RNK-polimerazy, na sperktr belkov, sinteziruemykh kletkami Escherichia coli.