Low ribonuclease activity in cellular lysates of osmotic sensitive Saccharomyces cerevisiae mutants.
Cellular lysates with very low total ribonuclease activities are obtained by lysis of Saccharomyces cerevisiae VY1160 osmotic sensitive mutant cells in 1% sorbitol solution. These lysates could be used for isolation of intact polysomes and messenger RNA molecules, or for studying of specific ribonucleases.
D002458:Cell Fractionation; D009154:Mutation; D009996:Osmotic Fragility; D012331:RNA, Fungal; D012260:Ribonucleases / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*
D012331:RNA, Fungal; D012260:Ribonucleases