Bacterial lipopolysaccharides bind selectively to lymphocytes from lipopolysaccharide high-responder mouse strains.
Three different concentrations of horseradish peroxidase-labelled lipopolysaccharide (LPS-HRP) were added in vitro to spleen cells from the LPS high-responder strain C3H/Tif and to cells from the low-responder strain C3H/HeJ. After being washed and fixed the cells were exposed to the substrate and prepared for electron microscopy. After addition of 7 and 0.7 microgram/ml of labelled LPS only lymphocytes from the high-responder strain were labelled. About 5-10% of the cells from C3H/Tif bound LPS, which is in accordance with the known frequency of B cells possessing the genetically determined LPS receptor. At the highest dose of labelled LPS (70 microgram/ml) a large proportion of lymphocytes from the low-responder strain also bound LPS. Erythrocytes from both strains bound LPS at all concentrations. It is concluded that LPS-HRP allows the detection at the cellular level of LPS binding to the genetically controlled membrane receptor for LPS.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D002478:Cells, Cultured; D004247:DNA / Q000096:biosynthesis; D004926:Escherichia coli; D006735:Horseradish Peroxidase / Q000378:metabolism; D007152:Immunologic Capping; D008070:Lipopolysaccharides / Q000276:immunology; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D011946:Receptors, Antigen / Q000032:analysis
D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D011946:Receptors, Antigen; D004247:DNA; D006735:Horseradish Peroxidase