title: Computer aided analysis of chromatin network and basophil color for differentiation of mononuclear peripheral blood cells.
abstract: Computer aided differentiation of plasmoblasts, Pfeiffer cells, immunoblasts, lymphocytes and centrocytes is achieved with the parameters of chromatin network arrangement and structure, and multispectral cytoplasm color. The digital methods involve: (a) segmenting the nuclear image into topographic sections and analyzing the optical density distribution from the chromatin in these sections; (b) determining the nuclear structure with a 7 x 7 median filter, gradient filter and contour following algorithms; and (c) clustering two-dimensional chromatic data from panoptically stained cellular components. The parameters reported here are a subset of those needed for the automated diagnosis of many hematologic diseases especially the leukemias.
mesh_terms: D001773:Blood Cells / Q000166:cytology*; D002467:Cell Nucleus / Q000648:ultrastructure*; D002843:Chromatin / Q000648:ultrastructure*; D003116:Color; D003201:Computers; D003584:Cytological Techniques; D006801:Humans; D010783:Photometry
chemical_list: D002843:Chromatin