[Transforming activity of plasmid R6K DNA on Serratia marcescens strain 20-10. The behavior of the plasmid in the transformants].
The authors described transformation of S. marcescens, strain 20-10, of the isolated R6K plasmide DNA. As demonstrated by centrifugation in cesium chloride gradient and electrophoresis in agarose, the plasmide was present in the transformants in the form identical to R6K in E. coli K12. Analysis of the transforming activity of R6K plasmide from Serratia and E. coli K12 strains with a complete and defective restriction system showed S. marsescens, strain 20-10, to possess specific system of restriction and modification. In studying beta-lactamase activity and Serratia and E. coli strains ampicillin and streptomycin resistance revealed differences in the phenotypical expression of the plasmide signs in the heterologous and homologous host.
D004269:DNA, Bacterial / Q000235:genetics*; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000235:genetics; D010641:Phenotype; D010957:Plasmids; D012706:Serratia marcescens / Q000187:drug effects* / Q000235:genetics; D014170:Transformation, Genetic / Q000187:drug effects*
D004269:DNA, Bacterial
Transformiruiushchaia aktivnost' DNK plazmidy R6K na shtamme Serratia marcescens 20-10. Povedenie plazmidy v transformantakh.