Genetic effects of impure and pure saccharin in yeast.
Yeast cells were grown in media containing impure or purified saccharin preparations. Dose-dependent increases in frequencies of cells possessing aberrant cell morphologies were revealed by light microscopy. At each test dose, cells grown in impure saccharin exhibited up to sevenfold higher frequencies of mitotic crossing-over or gene conversion in three of four assays for genetic recombination than cells grown in purified saccharin from the same lot. With one exception, the sweetener produced by the Maumee process caused larger increases in recombination and gene reversion than the sweetener produced by the Remsen-Fahlberg process. The several test markers did not respond equally to any test saccharin. Cells grown in liquid media containing no saccharin or two of three test concentrations of saccharin produced cell titers that were approximately equivalent.
D002455:Cell Division / Q000187:drug effects; D003434:Crossing Over, Genetic / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D008938:Mitosis / Q000187:drug effects; D009153:Mutagens; D011995:Recombination, Genetic / Q000187:drug effects; D012439:Saccharin / Q000138:chemical synthesis / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects*; D013329:Structure-Activity Relationship
D009153:Mutagens; D012439:Saccharin