Use of an enzyme immunoassay test for characterizing the A and M antigens of Brucella.
An enzyme immunoassay test was developed for detecting the A and M antigens of brucellae. One hundred fifteen isolates, including 96 strains of Brucella abortus, 5 strains of B. suis, 1 strain of B. melitensis and 1 strain of B. neotomae, were accurately serotyped with the enzyme immunoassay test. No reactions were observed with 11 isolates of B. canis and 1 isolate of B. ovis. This rapid serological test provides for considerable savings in time and materials as compared with the standard tube agglutination test.
D000372:Agglutination Tests; D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis*; D002002:Brucella / Q000145:classification*; D002006:Brucellosis / Q000382:microbiology*; D006801:Humans; D007124:Immunoenzyme Techniques
D000942:Antigens, Bacterial