Characterization of a picornavirus isolated from broiler chicks.
The rectal content of an apparently normal 1-week-old broiler chick yielded an unclassified cytopathogenic virus with cytopathic effects of the round type. It was identified as a picornavirus from the following: ribonucleic acid in the viral core; virus growth in the cytoplasm; a particle about 30 nm in diameter; resistance to ethyl ether, chloroform, trypsin, and acid; relative heat-lability; and partial stabilization to molar magnesium chloride. The virus was stable under freezing and thawing, and sonication. It was distinguished from avian encephalomyelitis virus by the neutralization test.
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens / Q000382:microbiology*; D003588:Cytopathogenic Effect, Viral; D005455:Fluorescent Antibody Technique; D010849:Picornaviridae / Q000254:growth & development / Q000276:immunology / Q000302:isolation & purification*; D012007:Rectum / Q000382:microbiology; D013696:Temperature; D014132:Trachea / Q000382:microbiology; D010948:Viral Plaque Assay