Red cell-linked antibody assay for detection of antigenic markers on cell surfaces.
A direct method for demonstrating antigenic markers on cell surface has been developed which makes use of the ability of bovine red blood cells coated with protein A of Staphylococcus aureus (ES) to binding IgG antibody without agglutinating. Red cell-linked rabbit IgG antibody reagents (ESA) were prepared in this way, and Ig-, IgM- and Thy-1-bearing mouse spleen and peripheral blood lymphocytes were identified by a rosette assay.
D000818:Animals; D000906:Antibodies; D002417:Cattle; D004912:Erythrocytes / Q000276:immunology*; D007074:Immunoglobulin G / Q000276:immunology; D007158:Immunologic Techniques; D051379:Mice; D008808:Mice, Inbred CBA; D011817:Rabbits; D011946:Receptors, Antigen / Q000032:analysis*; D011961:Receptors, Fc; D012397:Rosette Formation; D013205:Staphylococcal Protein A / Q000276:immunology
D000906:Antibodies; D007074:Immunoglobulin G; D011946:Receptors, Antigen; D011961:Receptors, Fc; D013205:Staphylococcal Protein A