The requirement of nonsense suppression for the development of several phages.
A spontaneous streptomycin-resistant Escherichia coli mutant which is temperature-sensitive for suppression of a nonsense codon was studied for its ability to propagate phages T2, T4D, T5, phi K, f2, MS2, R17, Q beta, lambda as well as filamentous phages fl, fd and M13. Of all phages tested, only the growth of Q beta, lambda, and filamentous phages is inhibited in the mutant at 42 degree C. This selective inhibition suggests that, like Q beta, lambda and filamentous phages also require a read-through proten(s) which results from suppression of a termination codon.
D003090:Coliphages / Q000254:growth & development*; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000235:genetics*; D005815:Genetic Code; D008242:Lysogeny; D009154:Mutation; D010443:Peptide Chain Termination, Translational; D010641:Phenotype; D013307:Streptomycin / Q000494:pharmacology; D013489:Suppression, Genetic
D013307:Streptomycin