An immunological approach to cell separation of the central nervous system.
In this paper I review the various steps of development required for an immunological approach to cell separation. Key points include choice of brain region, isolation procedure, ligand selection, functional assays for separated cell population, and selection of separation procedure. I review briefly some results we have obtained using cerebellar cell populations isolated from 10-day-mice.
D000818:Animals; D002469:Cell Separation; D002490:Central Nervous System / Q000276:immunology*; D002499:Centrifugation, Density Gradient; D002531:Cerebellum / Q000201:enzymology / Q000276:immunology; D002846:Chromatography, Affinity; D003337:Corpus Callosum / Q000276:immunology; D003851:Deoxyribonucleases / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D007106:Immune Sera / Q000494:pharmacology; D008024:Ligands; D008280:Magnetics; D051379:Mice; D008863:Microspheres; D011817:Rabbits; D014357:Trypsin / Q000494:pharmacology
D007106:Immune Sera; D008024:Ligands; D003851:Deoxyribonucleases; D014357:Trypsin