title: Detection of specific immunoreactivity by LAI assay in patients with malignant melanoma of uveal tissue.
abstract: To monitor the specific antitumor immunity to patients with malignant melanoma of the uveal tissue, use was made of the 20-hr modification of the leukocyte adherence inhibition (LAI) test. Simultaneously, changes in the proportionally of T (total and active) and B lymphocytes in peripheral blood were followed in the same patients by the E and EAC rosette formation test. Peripheral blood leukocytes from patients with malignant melanoma of the uvea, cultured in vitro for 20 hr with syngeneic and allogeneic tumor extract showed a significantly lower adhering capacity than the same cell population cultured without the specific tumor extract (21/22). A retest 3 months after tumor removal showed the percentage of positive cases to have declined (11/19). Leukocyte adherence of control donors, cultured with and without the tumor extract remained unchanged. A follow-up of immunocompetence in patients with malignant melanoma of the uvea, based on proportionally changes of total T, "active" T and B lymphocytes yielded information of an orienting value only. The results of investigation of the various cases proved to be considerably divergent and failed to present a reliable picture of the patient's immunological status.
mesh_terms: D001402:B-Lymphocytes / Q000276:immunology; D005134:Eye Neoplasms / Q000276:immunology*; D006801:Humans; D007158:Immunologic Techniques; D007957:Leukocyte Adherence Inhibition Test; D008545:Melanoma / Q000276:immunology*; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000276:immunology; D014603:Uveal Diseases / Q000276:immunology*