title: [Study of Leydig cells and gonadotropin activity in 14-18 days old fetal mouse (author's transl)].
abstract: An organ culture system has been developed for mouse embryo testes and pituitaries at 14-18 days of gestation. The testosterone (T) production by fetal testes has been measured by RIA in the culture medium: it increases from day 14 to day 18 and may be specifically stimulated by ovine LH and hCG. Fetal pituitaries in culture release a gonadotropin activity at 16 and 18 days (not found at 14 days), which is detectable by an heterologous RIA of LH and by a significant increase in T production from age-matched testes in co-culture. The 18-days old pituitaries respond to synthetic LH-RH by an enhanced production of immunologically and biologically active LH.
mesh_terms: D000818:Animals; D005260:Female; D005333:Fetus / Q000378:metabolism; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology; D006062:Gonadotropins / Q000494:pharmacology; D006065:Gonadotropins, Pituitary / Q000096:biosynthesis*; D007985:Leydig Cells / Q000378:metabolism*; D007986:Luteinizing Hormone / Q000096:biosynthesis; D008297:Male; D051379:Mice; D009924:Organ Culture Techniques; D010903:Pituitary Gland, Anterior / Q000378:metabolism; D011247:Pregnancy; D012733:Sex Differentiation; D013737:Testis / Q000378:metabolism; D013739:Testosterone / Q000096:biosynthesis
chemical_list: D006062:Gonadotropins; D006065:Gonadotropins, Pituitary; D007987:Gonadotropin-Releasing Hormone; D013739:Testosterone; D007986:Luteinizing Hormone
vernacular_title: Etude de l'activité leydigienne et gonadotrope chez l'embryon de souris mâle en fin de gestation.