title: Sequence analysis of two yeast mitochondrial DNA fragments containing the genes for tRNA Ser UCR and tRNA Phe UUY.
abstract: Two restriction enzyme fragments containing yeast mitochondrial tRNA genes have been characterized by DNA sequence analysis. One of these fragments is 320 base pairs long and contains a tRNA Ser gene. The corresponding tRNA SER was isolated from yeast mitochondria and its nucleotide sequence also was determined. This mitochondrial tRNA is 90 nucleotides in length, has a G + C content of 38%, and has UGA as the anticodon. A portion of a 680-base-pair DNA fragment containing a tRNA Phe gene was also sequenced. The portion of this gene which codes for the mature tRNA is 75 base pairs in length, has a G + C content of 33%, and contains the anticodon GAA. Neither gene contains an intervening sequence or codes for the 3' CCA terminus. Both are surrounded by regions of more than 90% A + T. The significance of these sequences is discussed.
mesh_terms: D001483:Base Sequence; D004262:DNA Restriction Enzymes; D004272:DNA, Mitochondrial / Q000378:metabolism; D005796:Genes; D005815:Genetic Code; D009693:Nucleic Acid Hybridization; D010649:Phenylalanine; D012343:RNA, Transfer / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D012694:Serine
chemical_list: D004272:DNA, Mitochondrial; D012694:Serine; D010649:Phenylalanine; D012343:RNA, Transfer; D004262:DNA Restriction Enzymes