[Production and use of a nutrient medium with egg hydrolysate for monolayer reproduction of BHK 21/13 cells].
Experiments aiming to reproduce BHK 21/13 cells in Roux retorts by use of a semi-synthetic nutrient medium, based on protein hydrolyzate synthetized by the authors, were carried out. Results obtained show that the lower peptides and free amino acids found in ovarian hydrolizate meet the requirements of the cell line in respect to quality and quantity. The successfull cultivation of BHK 21/13 cells in hydrolyzate medium made possible a considerable reduction in the percentage involvement of serum, which is usually used in the classical nutrient media of the type Eagle BME intended for the propagation of diploid cell strains.
D000818:Animals; D002455:Cell Division; D002460:Cell Line; D002478:Cells, Cultured / Q000378:metabolism*; D002850:Chromatography, Gel; D006224:Cricetinae; D003470:Culture Media / Q000378:metabolism*; D004531:Eggs; D007668:Kidney; D010447:Peptide Hydrolases / Q000378:metabolism; D011492:Protein Hydrolysates / Q000378:metabolism*; D013379:Substrate Specificity
D003470:Culture Media; D011492:Protein Hydrolysates; D010447:Peptide Hydrolases
Poluchavane i izpolzvane na khranitelna sreda s iaĭchen khidrolizat za monosloĭno razmnozhavane na kletki BHK 21/13.