[Application of lytic enzymes for disruption of microbial cell walls to isolate DNA].
The possibility of isolating DNA from yeast and actinomycete cells, previously exposed to enzymic disruption, was explored. The enzyme was produced from Actinomyces griseinus II. The highest yield of DNA from microbial cells was obtained as a result of detergent pretreatment of the yeast and actinomycetes and subsequent disruption of cell walls with the above enzyme.
D000190:Actinomyces / Q000032:analysis* / Q000201:enzymology; D004271:DNA, Fungal / Q000302:isolation & purification*; D012441:Saccharomyces cerevisiae / Q000032:analysis*; D013696:Temperature
D004271:DNA, Fungal
Primenenie preparata liticheskikh fermentov dlia razrusheniia kletochiykh stenok mikroorganizmov s-tsel'iu vydeleniia iz nikh DNK.