title: Inversion in the lactose region of Escherichia coli K-12.
abstract: A spontaneous mutant of Escherichia coli K-12, strain SY99, with an inversion in the lactose region was isolated and partially characterized. The inversion was detected due to inverse chromosomal conjugational transfer after introduction of an F42 (F'lac) episome. The termini of the inversion are between proAB and lac on one side and lac and proC on the other. The inverse conjugational transfer in SY99 did not appear to be absolute but was always accompanied by a residual "normal" counterclockwise mobilization. This residual transfer was further shown to be caused by the intrinsic instability of this region (at least in the line W3110). The possible involvement of IS3 elements flanking the lactose operon is discussed.
mesh_terms: D002876:Chromosomes, Bacterial / Q000502:physiology*; D003227:Conjugation, Genetic; D004926:Escherichia coli / Q000235:genetics*; D005144:F Factor; D005819:Genetic Markers; D007763:Lac Operon; D011995:Recombination, Genetic
chemical_list: D005819:Genetic Markers