title: Nucleotide sequence of the three major early promoters of bacteriophage T7.
abstract: I have determined the nucleotide sequences of the three major early promoters of bacteriophage T7 (A1, A2, A3). The sequences confirm the two main homologies found between other known promoters for E. coli RNA polymerase (nucleoside triphosphate:RNA nucleotidyl transferase, E.C. 2. 7. 7. 6). In particular, all three T7 promoters show a very good match with the -35 region homology; the A2 and A3 promoters share a 17 basepair sequence in this region. On the other hand, the match with the Pribnow Box homology is much less pronounced and different for each T7 promoter.
mesh_terms: D001483:Base Sequence; D003090:Coliphages / Q000378:metabolism*; D004262:DNA Restriction Enzymes; D004279:DNA, Viral / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004926:Escherichia coli / Q000201:enzymology*; D009838:Oligodeoxyribonucleotides / Q000032:analysis; D014176:Protein Biosynthesis; D014158:Transcription, Genetic
chemical_list: D004279:DNA, Viral; D009838:Oligodeoxyribonucleotides; D012321:DNA-Directed RNA Polymerases; D004262:DNA Restriction Enzymes