title: The use of fluorescent labeled antigen to follow the fate of instilled immune complexes in the rabbit lung.
abstract: To follow the fate of exogenously introduced immune complexes in the rabbit lung, a direct labeling method was devised. Bovine serum albumin was incubated with fluorescein isothiocyanate, washed and then bound at equivalence with antibovine serum albumin. Immune complexes thus prepared are injected into a rabbit airway and the animal was sacrificed. Using chilled alcohol fixative and paraffin embedding 4 micrometer sections were prepared for light and fluorescent microscopy. This technique readily verifies that labeled antigen is taken up by alveolar macrophages. Electron microscopy using ferritin-antiferritin complexes corroborate these light findings.
mesh_terms: D000818:Animals; D000936:Antigen-Antibody Complex; D000941:Antigens; D002417:Cattle; D005455:Fluorescent Antibody Technique; D007158:Immunologic Techniques; D008168:Lung / Q000276:immunology* / Q000648:ultrastructure; D011817:Rabbits; D012710:Serum Albumin, Bovine
chemical_list: D000936:Antigen-Antibody Complex; D000941:Antigens; D012710:Serum Albumin, Bovine