Platinum(II) complexes block the entry of T4 phage DNA into the host cells.
The efficiency of multiplicity reactivation of T4 particles inactivated by platinum(II) complexes is very low. The same is true for marker rescue and functional survival of genes. This can be at least partly explained by the inability of most inactivated virus particles to introduce their DNA into the host cells as demonstrated by electron microscopy. Conformational changes in the DNA, formation of DNA-DNA and DNA-protein cross-links and the damage of proteins participating in the injection process could be responsible for the phenomenon observed.
D002945:Cisplatin / Q000494:pharmacology*; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000187:drug effects*; D005819:Genetic Markers / Q000187:drug effects; D008854:Microscopy, Electron; D013604:T-Phages / Q000378:metabolism* / Q000648:ultrastructure
D004269:DNA, Bacterial; D005819:Genetic Markers; D002945:Cisplatin