Participation of a histamine-Sepharose-adherent subpopulation of human mononuclear cells in the production of leucocyte migration inhibition factor (LIF) in healthy children.
The separation of mouse splenic T lymphocytes into distinct subpopulations by fractionation on histamine-rabbit serum albumin Sepharose (H-RSAS) columns has been described. The H-RSAS-adherent T cells have been attributed regulatory functions associated with B cell activity, T cell-mediated cytotoxicity and the secretion of mediators such as immuno-interferon. The possibility that H-RSAS-adherent T cells exert a similar regulatory effect on an in vitro parameter of T cell-mediated immunity was investigated by assaying the production of leucocyte migration inhibition factor (LIF) in human blood samples, using the agarose droplet method. Phytohaemagglutinin (PHA) and BCG-purified protein derivative (PPD) were used as stimulants of LIF secretion which was measured as a percentage of inhibition of linear leucocytic migration. In normal individuals a highly significant (P less than 0.001) decrease was demonstrated in the production of LIF by peripheral blood leucocytes depleted of H-RSAS-adherent cells. Migration inhibition dropped from 36 +/- 11.7% to 21.2 +/- 12.9% in eighteen cases tested with PHA and from 29.3 +/- 11.7% to 17.2 +/- 9.8% in twelve cases tested with PPD. These results suggest the existence of a lymphocytic subpopulation involved in LIF production which expresses histamine receptors.
D002448:Cell Adhesion; D002469:Cell Separation; D002648:Child; D002675:Child, Preschool; D006632:Histamine; D006801:Humans; D007223:Infant; D007961:Leukocyte Migration-Inhibitory Factors / Q000096:biosynthesis*; D007962:Leukocytes / Q000378:metabolism*; D008222:Lymphokines / Q000096:biosynthesis*; D010835:Phytohemagglutinins / Q000494:pharmacology; D012685:Sepharose; D013601:T-Lymphocytes / Q000378:metabolism; D014373:Tuberculin / Q000494:pharmacology
D007961:Leukocyte Migration-Inhibitory Factors; D008222:Lymphokines; D010835:Phytohemagglutinins; D014373:Tuberculin; D006632:Histamine; D012685:Sepharose