The isolation and genetic characteristics of lambda transducing phages of the uvrA+ and uvrC+ genes of E. coli K12.
Lambda transducing phages carrying the excision repair genes uvrA+ and uvrC+ were selected from a pool of lambda phages carrying EcoR1 fragments of E. coli DNA. These phages and also lambdauvrB+ (obtained from Gottesman) were used to make lysogens of excision-defective strains carrying uvrA-, uvrB- or uvrC-. Lambda uvrA+ was found to transduce strains carrying uvrA- but not those carrying uvrB- or uvrC-, to normal ultraviolet resistance. Similarly, lambdauvrB+ and lambdauvrC+ were found to complement only the corresponding uvr- allele. The lambda transducing phages were co-transduced with gal+ by P1 phage into lysogenic gal- recipients, and presumably were integrated at the normal prophage site.
D000483:Alleles; D002876:Chromosomes, Bacterial; D003090:Coliphages / Q000235:genetics*; D004260:DNA Repair; D004262:DNA Restriction Enzymes / Q000235:genetics; D004269:DNA, Bacterial / Q000528:radiation effects; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D005814:Genes, Viral; D008242:Lysogeny; D014161:Transduction, Genetic; D014466:Ultraviolet Rays
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes