title: A comparison of flushing fluids for initial perfusion of kidneys for transplantation.
abstract: Four solutions for initial flushing of kidneys prior to transplantation were tested under conditions designed to resemble those of clinical cadaveric donor renal transplantation. The experimental model was the dog subjected to bilateral nephrectomy with renal autograft. Kidney grafts were subjected to 15 minutes' anoxia in vivo, 30 minutes' warm ischaemia at 37 degrees C ex vivo, and two hours' cold ischaemia before reimplantation. The four solutions used were Collins (C3), Perfudex (P), hyperosmolar citrate (HC), and a solution of bovine albumin containing dog red blood cells (BBA). Effects of the flushing fluids were compared by parameters relating to dog survival, renal function, and serum enzyme levels. With all parameters studied the best results occurred in HC perfused kidneys. Results with BBA perfusion were marginally worse, while C3 perfused kidneys were again inferior. P perfused kidneys clearly did least well. The results support the use of HC for clinical application.
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004285:Dogs; D004798:Enzymes / Q000097:blood; D004912:Erythrocytes / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006982:Hypertonic Solutions; D007668:Kidney / Q000378:metabolism; D016030:Kidney Transplantation; D008278:Magnesium Sulfate / Q000378:metabolism; D009994:Osmolar Concentration; D010101:Oxygen Consumption / Q000187:drug effects; D010477:Perfusion / Q000379:methods*; D011343:Procaine / Q000378:metabolism; D014184:Transplantation, Homologous
chemical_list: D004798:Enzymes; D006982:Hypertonic Solutions; D011343:Procaine; D008278:Magnesium Sulfate; D005947:Glucose