Immunologic monitoring of transplant rejection: correlation of in vitro assays with morphologic changes on transplant biopsy.
The assays of lymphocyte-mediated cytotoxicity (LMC), antibody-dependent cell-mediated cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC) were correlated with histopathologic criteria of refection in 35 transplant biopsies. A positive LMC was seen with 6/8 biopsies showing moderate to severe cellular rejection and in 8/17 with mild cellular rejection. Positive ADCC and/or CDC assays were associated with 14/14 biopsies containing rejection vasculitis. These results suggest that selected in vitro assays may be useful in monitoring the immunologic events occurring in the rejecting allograft.
D000920:Antibody-Dependent Cell Cytotoxicity; D001706:Biopsy; D003165:Complement System Proteins; D006084:Graft Rejection; D006801:Humans; D066298:In Vitro Techniques; D007668:Kidney / Q000473:pathology; D016030:Kidney Transplantation; D008214:Lymphocytes / Q000276:immunology; D011183:Postoperative Complications; D014184:Transplantation, Homologous; D014657:Vasculitis / Q000209:etiology
D003165:Complement System Proteins