[The microbiological cancer test--further studies with experimental mouse tumor tetanus and wound tetanus].
A re-evaluation of the specificity of the tumour-tetanus assay of the mouse was performed by analysing the wound-tetanus assay under comparable test conditions. This was achieved by injecting 1 X 10(6) viable Ehrlich carcinoma cells admixed with 1 X 10(2) tetanus spores subcutaneously, in a 0.1 ml dose volume or, 1 X 10(2) tetanus spores suspended in 5% CaCl2 solution, respectively. By comparison, these two groups of mice developed about the same tetanus mortality rates, however, following tetanus antitoxin therapy with 3 doses of 100 IU each day on days 0, 4 and 7 after infection, clinical signs of late tetanus exclusively belonged to tumour bearing animals. This typical tetanus behaviour may be explained by a spatial-temporal association between growing tetanus rods and proliferating cells of warm-blooded animals. In this manner tumour tissue can be differed from wound granulomatous tissue by way of permanently cloning stem cells.
D000818:Animals; D002122:Calcium Chloride; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism*; D003017:Clostridium tetani / Q000378:metabolism*; D008297:Male; D051379:Mice; D008938:Mitosis; D013742:Tetanus / Q000401:mortality*; D013743:Tetanus Antitoxin / Q000494:pharmacology; D013744:Tetanus Toxin / Q000096:biosynthesis
D013743:Tetanus Antitoxin; D013744:Tetanus Toxin; D002122:Calcium Chloride
Der mikrobiologische Krebstest - weiterführende Untersuchungen mit dem experimentellen Tumor-Tetanus und Wund-Tetanus der Maus.