Assay for mutagenicity of bile in Sprague-Dawley rats treated subcutaneously with intestinal carcinogens.
To investigate the mode of action of sc injected intestinal carcinogens, the mutagenicity assay of bile collected from noninbred Sprague-Dawley rats treated sc with carcinogens was conducted in the presence and absence of beta-glucuronidase. The bile samples from rats inoculated with 4-aminobiphenyl were mutagenic for Salmonella typhimurium TA100 only in the presence of beta-glucuronidase, whereas those from the 3,2'-dimethyl-4-aminobiphenyl-treated rats did not require the enzyme for mutagenicity toward strain TA100. On the contrary, the assays with S. typhimurium G46 and TA100 of bile from rats inoculated with 1,2-dimethylhydrazine, azoxymethane, or methylazoxymethanol acetate failed to reveal mutagenicity whether beta-glucuronidase was added or not, though these carcinogens were highly mutagenic for strain G46 in the Salmonella-microsome mutagenicity test and/or in the host-mediated assay.
D000818:Animals; D001397:Azoxymethane / Q000494:pharmacology; D001646:Bile / Q000378:metabolism*; D002273:Carcinogens / Q000378:metabolism / Q000494:pharmacology*; D004127:Dimethylhydrazines / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D005966:Glucuronidase / Q000378:metabolism; D066298:In Vitro Techniques; D007279:Injections, Subcutaneous; D008297:Male; D008746:Methylazoxymethanol Acetate / Q000494:pharmacology; D008862:Microsomes, Liver / Q000378:metabolism; D009153:Mutagens; D051381:Rats; D012486:Salmonella typhimurium / Q000187:drug effects
D002273:Carcinogens; D004127:Dimethylhydrazines; D009153:Mutagens; D008746:Methylazoxymethanol Acetate; D005966:Glucuronidase; D001397:Azoxymethane