Gonadotroph-rich cell lines derived from pituitary clonal cells (2A8) grafted under the kidney capsule.
Gonadotroph-rich cell lines were established from multipotential pituitary clonal cells (2A8) which were implanted under kidney capsule of hypophysectomized female rats. These cell lines secrete gonadotrophins (FSH and LH) continuously over two months after establishment; LHRH stimulated the secretion of hormones into the culture medium. Many of the cells reacted immunohistochemically to antiserum to FSH or LH, while a small number reacted to antiserum to prolactin or TSH. They did not contain normal secretory granules such as those of gonadotrophs in vivo.
D000818:Animals; D002460:Cell Line; D002999:Clone Cells; D003594:Cytoplasmic Granules / Q000648:ultrastructure; D005260:Female; D005640:Follicle Stimulating Hormone / Q000378:metabolism*; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology; D007016:Hypophysectomy; D007668:Kidney / Q000601:surgery; D007986:Luteinizing Hormone / Q000378:metabolism*; D010903:Pituitary Gland, Anterior / Q000166:cytology* / Q000637:transplantation / Q000648:ultrastructure; D051381:Rats; D014184:Transplantation, Homologous
D007987:Gonadotropin-Releasing Hormone; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone