Regulation of mitogen- and antigen-stimulated lymphocyte blastogenesis by prostaglandins.
Exogenously added prostaglandin E1 or E2 inhibited the blastogenic response of Mycobacterium bovis-sensitized bovine peripheral blood lymphocytes stimulated with concanavalin A, phytohemagglutinin, or M. bovis purified protein derivative as measured by [3H]thymidine uptake. The kinetics of the response showed that prostaglandins must be added to lymphocyte cultures within hours after mitogen or antigen addition to achieve maximum suppression of [3H]thymidine uptake. Addition of prostaglandins 24 h after the addition of mitogens or antigens resulted in considerably less suppression, supporting a hypothesis that prostaglandins initiate an early series of events which ultimately control lymphocyte blastogenesis rather than directly inhibit deoxyribonucleic acid synthesis.
D000818:Animals; D000942:Antigens, Bacterial; D001095:Arachidonic Acids / Q000494:pharmacology; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D003208:Concanavalin A; D008213:Lymphocyte Activation / Q000187:drug effects*; D008214:Lymphocytes / Q000378:metabolism; D009163:Mycobacterium bovis / Q000276:immunology; D010835:Phytohemagglutinins; D011458:Prostaglandins E / Q000494:pharmacology*; D013936:Thymidine / Q000378:metabolism
D000942:Antigens, Bacterial; D001095:Arachidonic Acids; D010835:Phytohemagglutinins; D011458:Prostaglandins E; D003208:Concanavalin A; D013936:Thymidine