A comparison of methods for obtaining high yields of pure immunoglobulin from severely haemolysed plasma.
Ammonium sulphate, DEAE ion exchange chromatography, and polyethylene glycol (PEG) were compared for their ability to isolate and purify large quantities of immunoglobulins from severely haemolysed plasma. PEG, which produced the highest yield and purity, proved to be the only method which completely removed the haemoglobin.
D000645:Ammonium Sulfate; D011232:Chemical Precipitation; D002852:Chromatography, Ion Exchange / Q000379:methods; D006461:Hemolysis; D006801:Humans; D007136:Immunoglobulins / Q000302:isolation & purification*; D007158:Immunologic Techniques; D011092:Polyethylene Glycols
D007136:Immunoglobulins; D011092:Polyethylene Glycols; D000645:Ammonium Sulfate