Indirect hemagglutination using whole mixed antigen for checking toxoplasmosis immunity and for serodiagnosis of human toxoplasmosis, compared with immunofluorescence.
The indirect hemagglutination (IHA) is an easy technic for which it is possible to get commercial reactifs. This methode should be used often for the serodepistage of Toxoplasmosis and control of the immunitry anti-toxoplasmosis. We used a technique with total mixed antigen, that was compared with indirect immuno-fluorescence (I.F.I.), for 623 human serums. The I.H.A. was used with sheep formal hematies and coated with glutaraldehyde, with a total mixed antigen prepared from ultra sonicated parasites. This standardised reactive can be kept one year at 4 degrees C. We worked with U well micro-titration plates and the result is obtained after 2 hours. The I.F.I. technique was made with classical methods using Evans blue counter staining. The reproducibility of the I.H.A., during 366 tests was satisfactory. For the control of the immunity anti-toxoplasmic, the results tallied with both methods in more 95% of cases. Lastly, as test of progression, the I.H.A. with total mixed antigen appear an interesting way for the early diagnosis of the Toxoplasmosis where the reaction seems to be positive as soon as I.F.I.
D000941:Antigens / Q000276:immunology; D005455:Fluorescent Antibody Technique; D006386:Hemagglutination Tests / Q000379:methods; D006801:Humans; D007109:Immunity; D014123:Toxoplasmosis / Q000175:diagnosis / Q000276:immunology*
D000941:Antigens