The correlation of kidney transplant survival with lymphocyte mediated cytotoxicity on B cell targets.
A LMC micromethod using specific B lymphocyte target cells labeled with a fluorescent dye has been developed to select matched donors and recipients for kidney allotransplantation. The fluorescence of the B lymphocyte target cells was 85% or less of control levels in 9 of 28 patients. In 8 of these 9 hosts graft loss occurred. Nineteen sets of target cells showed 90-95% of control fluorescence. Only 5 of these recipients lost their transplants (p less than 0.01). The fluorescent LMC test correlates with successful renal transplant outcome when the target cells are purified B cells.
D000937:Antigen-Antibody Reactions; D001402:B-Lymphocytes / Q000276:immunology*; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods*; D006085:Graft Survival; D006801:Humans; D016030:Kidney Transplantation; D013601:T-Lymphocytes / Q000276:immunology; D014019:Tissue Donors; D014184:Transplantation, Homologous