title: Scanning immunoelectron microscopy for the identification and mapping of two or more antigens on cell surfaces.
abstract: This study demonstrates the feasibility of using scanning electron microscopy for the identification and mapping of two or more antigens on cell surfaces. Three types of cells: human type B red cells, tumor cells and their associated virus, and guinea pig hepatocarcinoma cells were immunolabeled with ferritin or gold conjugated to IgG fractions of antigen-specific antisera. Gold particles, 300-500 A in diameter, were conjugated to an IgG fraction either directly or indirectly using Staphylococcal protein A. Gold particles of this size can be distinguished from ferritin label using a high resolution scanning microscope. Correlative light, transmission electron microscopy, and scanning electron microscopy studies, as well as x-ray diffraction analysis and the study of stereo micrographs, were performed to visualize the simultaneous immunolabeling by ferritin and gold of two antigens on the guinea pig hepatocarcinoma cell.
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000032:analysis*; D002462:Cell Membrane / Q000648:ultrastructure; D002478:Cells, Cultured; D003102:Colloids; D004912:Erythrocytes / Q000276:immunology / Q000648:ultrastructure; D005293:Ferritins; D006046:Gold; D006801:Humans; D007152:Immunologic Capping; D007158:Immunologic Techniques; D051379:Mice; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D013205:Staphylococcal Protein A
chemical_list: D000954:Antigens, Surface; D003102:Colloids; D013205:Staphylococcal Protein A; D006046:Gold; D005293:Ferritins