Identification of tubulin from the yeast Saccharomyces cerevisiae.
A tubulin-like protein was identified in the lower eukaryote Saccharomyces cerevisiae. The following criteria were used: (i) copolymerization of the 35S-labeled yeast protein with porcine brain tubulin; (ii) immunoprecipitation of the 35S-labeled yeast protein with antiflagellar tubulin antibody; (iii) the presence of the yeast protein as a constituent of isolated yeast nuclei; and (iv) splitting of the yeast protein in a gel electrophoretic system containing sodium dodecyl sulfate that resolved the alpha- and beta-tubulin chains from other sources. This protein did not appear to have significant affinity for the plant alkaloid, Colcemid.
D002467:Cell Nucleus / Q000032:analysis; D002850:Chromatography, Gel; D003703:Demecolcine / Q000378:metabolism; D006023:Glycoproteins / Q000032:analysis*; D011863:Radioimmunoassay; D012441:Saccharomyces cerevisiae / Q000032:analysis*; D014404:Tubulin / Q000032:analysis*
D006023:Glycoproteins; D014404:Tubulin; D003703:Demecolcine