Detection of antibody-forming cells and humoral antibody to horseradish peroxidase.
Two new simple methods for detecting antibody-forming cells by hemolytic plaque assay and hemagglutinating antibody to horseradish peroxidase have been developed in mice. Both techniques utilize as target, sheep erythrocytes coupled directly with horseradish peroxidase. These assays are sensitive, antigen-specific and are useful to quantitate both direct and indirect antibody-forming cells and humoral antibodies.
D000818:Animals; D000906:Antibodies; D000921:Antibody-Producing Cells / Q000276:immunology*; D001667:Binding, Competitive; D004136:Dinitrobenzenes / Q000276:immunology; D004306:Dose-Response Relationship, Immunologic; D006386:Hemagglutination Tests; D006433:Hemocyanins / Q000276:immunology; D006462:Hemolytic Plaque Technique; D006735:Horseradish Peroxidase / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D011817:Rabbits; D012756:Sheep; D013045:Species Specificity
D000906:Antibodies; D004136:Dinitrobenzenes; D006433:Hemocyanins; D006735:Horseradish Peroxidase