The effects of organic solvents and temperature on the desorption of yeast 3-phosphoglycerate kinase from immunoadsorbent.
Physicochemical parameters governing the elution of yeast 3-phosphoglycerate kinase from its immunoadsorbent were studied. Non-denaturing elution conditions were determined (alkali medium containing 50%, v/v, ethylene glycol) and the method was applied to one-step isolation of enzyme from a crude yeast preparation.
D000818:Animals; D000936:Antigen-Antibody Complex; D007106:Immune Sera / Q000494:pharmacology; D007164:Immunosorbents; D010735:Phosphoglycerate Kinase / Q000276:immunology* / Q000302:isolation & purification; D011817:Rabbits; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000276:immunology; D012997:Solvents / Q000494:pharmacology*; D013696:Temperature
D000936:Antigen-Antibody Complex; D007106:Immune Sera; D007164:Immunosorbents; D012997:Solvents; D010735:Phosphoglycerate Kinase