Cytostatic and phagocytic capacity of lymphokine-activated human monocytes.
Human monocytes were rendered cytostatic to the human cell line NHIK 3025 by exposure to lymphokine supernatants (LS) from BCG-stimulated lymphocytes. Exposure to LS for 1, 2 and 4 h induced a considerable cytostatic capacity in the monocytes. However, a stronger cytostatic effect was acquired by exposure to LS for 24 h and 72 h. The phagocytosis of 125I-labelled Candida albicans by LS-activated monocytes was compared with phagocytosis by monocytes treated with control supernatants (CS). The ingestion was increased by short exposure to LS. However, a 72-h exposure to LS induced a decreased ingestion capacity. The capacity of the LS-activated monocytes to digest ingested C. albicans was suppressed. DNA synthesis was increased in the LS-activated monocytes, while protein synthesis was not significantly influenced. The cytostatic capacity of LS-activated monocytes was abolished by culture for 24 h after removal of LS. Following removal of LS or CS with subsequent culture for 4 days, morphological and functional signs of differentiation were less marked in the LS-treated than in the CS-treated cells. The survival was also reduced in the former cells. However, these cells were strongly reactivated by re-exposure to LS.
D001500:BCG Vaccine / Q000494:pharmacology; D002176:Candida albicans / Q000254:growth & development; D002460:Cell Line; D002470:Cell Survival; D002478:Cells, Cultured; D004273:DNA, Neoplasm / Q000096:biosynthesis; D006801:Humans; D008213:Lymphocyte Activation; D008222:Lymphokines / Q000494:pharmacology*; D009000:Monocytes / Q000276:immunology*; D009369:Neoplasms / Q000378:metabolism / Q000473:pathology; D010587:Phagocytosis; D014176:Protein Biosynthesis
D001500:BCG Vaccine; D004273:DNA, Neoplasm; D008222:Lymphokines