Effects of diet upon hypothalamic vulnerability to goldthioglucose in male mice.
Three groups of male mice were fed three different diets for about 3 weeks: powdered chow (C), a high fat (F) diet (chow:Crisco, 2:1), and a high dextrose (D) diet (chow:dextrose, 2:1). Food and water intake and body weights (BWts) were measured daily. On Day 20 of the study, mice were injected IP with 0.3 mg/g of goldthioglucose (GTG). Two days later, the mice were sacrificed and brains were removed for histological examination of GTG-induced lesions in the ventromedial hypothalamus (VMH). It was found that (1) F-fed mice ate more and gained more BWt than C- and D-fed mice, and (2) the average GTG lesion volume of F-fed mice was twice as large as those of C- and D-fed mice. In view of known alterations in sensitivity of the VMH to goldthioglucose with changes in glucose metabolism, it is suggested that the larger goldthioglucose induced lesions in F-fed mice may be due to elevated blood levels of goldthioglucose or altered glucose metabolism in goldthioglucose sensitivity cells in the VMH.
D000818:Animals; D006051:Aurothioglucose / Q000494:pharmacology*; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D004032:Diet; D004041:Dietary Fats / Q000008:administration & dosage; D004326:Drinking / Q000187:drug effects; D004435:Eating / Q000187:drug effects; D002149:Energy Intake / Q000187:drug effects; D005947:Glucose / Q000008:administration & dosage; D006046:Gold / Q000494:pharmacology*; D006624:Hippocampus / Q000187:drug effects; D007031:Hypothalamus / Q000187:drug effects*; D007033:Hypothalamus, Middle / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D011301:Preoptic Area / Q000187:drug effects; D013495:Supraoptic Nucleus / Q000187:drug effects
D001786:Blood Glucose; D004041:Dietary Fats; D006051:Aurothioglucose; D006046:Gold; D005947:Glucose