Physical and coding properties of poly(5-methoxyuridylic) acid.
The synthesis of poly(mo5U) requires a high concentration (2.7 mg/ml) of polynucleotide phosphorylase as well as a long reaction time (48 h). The resulting polynucleotide has a chain length of approximately 100 nucleotides. It shows no indication of a stable secondary structure. When poly(mo5U) is mixed with poly(A), a triple-stranded complex poly(A) . 2poly(mo5U) is formed. This complex has a melting temperature of 68.5 +/- 0.5 degrees C at 150 mMNa+ and exhibits a hysteresis loop between melting and reformation of the complex having a delta Tm of 11.5 degrees C. Poly-5-methoxyuridylic acid stimulates the binding of Phe-tRNA to 70-S ribosomes but is inactive in directing poly(Phe) synthesis.
D004926:Escherichia coli / Q000378:metabolism; D007700:Kinetics; D008837:Micrococcus / Q000201:enzymology; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D009695:Nucleic Acid Renaturation; D010649:Phenylalanine; D011072:Poly U / Q000494:pharmacology; D011117:Polyribonucleotide Nucleotidyltransferase; D012343:RNA, Transfer / Q000378:metabolism; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet
D011072:Poly U; D010649:Phenylalanine; C019532:poly(5-methoxyuridylic) acid; D012343:RNA, Transfer; D011117:Polyribonucleotide Nucleotidyltransferase