Isolation of granules and vesicles rich in LHRH and catecholamines from the hypothalamus of the female rabbit.
In the female rabbit hypothalamus (HYP) the luteinizing hormone-releasing hormone (LHRH) and catecholamines (CAT) are mostly present in the granules and vesicles. A fraction of granules containing LHRH and vesicles containing CAT was obtained with 70% purity by ultracentrifugation in sucrose gradient. This fraction was devoid of synaptosomes.
D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D003594:Cytoplasmic Granules / Q000032:analysis* / Q000648:ultrastructure; D004298:Dopamine / Q000032:analysis; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000032:analysis*; D007031:Hypothalamus / Q000648:ultrastructure*; D008854:Microscopy, Electron; D009638:Norepinephrine / Q000032:analysis; D011817:Rabbits; D013572:Synaptic Vesicles / Q000032:analysis / Q000648:ultrastructure
D007987:Gonadotropin-Releasing Hormone; D004298:Dopamine; D009638:Norepinephrine