Specific heavy metal labeling of the 3-'terminus of phosphorothioate modified yeast tRNAPhe.
Yeast tRNAPhe containing a phosphorothioate modified -CS-CS-A terminus binds two moles of chloroterpyridineplatinum(II). This result was determined by titrating the tRNA with [3H](terpy)PtCl] Cl, removing excess platinum by cation exchange chromatography, and determining the amount of bound platinum by radiocounting techniques. It has thus been established that adjacent phosphorothioate modified nucleotides can be labeled with an electron dense stain, a necessary requirement for electronmicroscopic sequencing of polynucleotides to become practical.
D007700:Kinetics; D009944:Organoplatinum Compounds; D009946:Organothiophosphorus Compounds; D010649:Phenylalanine; D011725:Pyridines; D012343:RNA, Transfer; D012441:Saccharomyces cerevisiae; D014316:Tritium
D009944:Organoplatinum Compounds; D009946:Organothiophosphorus Compounds; D011725:Pyridines; D014316:Tritium; D010649:Phenylalanine; C021975:chloroterpyridineplatinum(II); D012343:RNA, Transfer