Measurement of 6-oxo-PGF1 alpha in human plasma using gas chromatography-mass spectrometry.
The plasma concentration of the prostacyclin (PGI2) hydration product 6-oxo-PGF1 alpha has been assayed by stable isotope dilution GC-MS in six normal volunteers infused with increasing doses of PGI2 intravenously. The predosing levels of 6-oxo-PGF1 alpha ranged between 114 and 266 pg/ml. Infusion of PGI2 increased 6-oxo-PGF1 alpha concentration in plasma but the increments were lower than expected suggesting less conversion of the PGI2 to 6-oxo-PGF1 alpha at high infusion rates.
D015121:6-Ketoprostaglandin F1 alpha; D011464:Epoprostenol / Q000097:blood; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D008297:Male; D011460:Prostaglandins F / Q000097:blood*
D011460:Prostaglandins F; D015121:6-Ketoprostaglandin F1 alpha; D011464:Epoprostenol