Reversal of methotrexate binding to dihydrofolate reductase by dihydrofolate. Studies with pure enzyme and computer modeling using network thermodynamics.
D001667:Binding, Competitive; D003201:Computers; D004926:Escherichia coli / Q000201:enzymology; D005492:Folic Acid / Q000031:analogs & derivatives*; D007700:Kinetics; D008433:Mathematics; D008727:Methotrexate / Q000494:pharmacology; D011485:Protein Binding; D013762:Tetrahydrofolate Dehydrogenase / Q000378:metabolism; D013816:Thermodynamics; D014316:Tritium
D014316:Tritium; C010920:dihydrofolate; D005492:Folic Acid; D013762:Tetrahydrofolate Dehydrogenase; D008727:Methotrexate