title: [Role of RNA-polymerase in gene activity regulation of E. coli RNA-polymerase mutants with a pleiotropic effect. I. Physiological and biochemical studies].
abstract: Four Rifr-mutants of E. coli B/r (rpo B401, rpo B402, rpo B403, rpo B409) which differ from the wild strain in one or more phenotypic properties besides rifampicin resistance were obtained. Transfer of the mutant Rifr-alleles into the parent strain gives the latter all the properties of the mutant. This indicates that the new properties are due to the pleiotropic effect of Rifr-mutations. Biochemical studies of the properties of RNA-polymerases from the mutants and the parent showed that some new properties of the mutants could not be explained by the appearance of analogous properties in the mutant RNA-polymerase itself. They seem to be caused by alteration in functional activity of the mutant enzyme, particulary, alteration of its control properties during transcription. The function of the beta-subunit in genetic transcription is discussed.
mesh_terms: D004269:DNA, Bacterial / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000235:genetics / Q000378:metabolism*; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D007700:Kinetics; D009154:Mutation; D010641:Phenotype; D012293:Rifampin / Q000494:pharmacology; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D012321:DNA-Directed RNA Polymerases; D012293:Rifampin
vernacular_title: Rol' RNK-polimerazy v reguliatsii aktivnosti genov u Escherichia coli RNK-polimeraznye mutanty s pleiotropnym éffektom. I. Fiziologicheskie i biokhimicheskie issledovaniia.