A new structural protein located in the Z lines of chicken skeletal muscle.
A new structural protein was purified from a prolonged 0.6 M KI extract of residues of chicken skeletal myofibrils, from which myosin, actin, and some other proteins had been removed. The protein had a chain weight of 55,000. The indirect immunofluorescence technique using antiserum against the 55,000 dalton protein revealed that the protein was exclusively located in the Z lines of a myofibril. The new protein formed lattice structures in vitro which were similar to those observed in the Z lines in situ.
D000818:Animals; D002645:Chickens; D005455:Fluorescent Antibody Technique; D008854:Microscopy, Electron; D008970:Molecular Weight; D009124:Muscle Proteins / Q000032:analysis*; D009132:Muscles / Q000648:ultrastructure; D009210:Myofibrils / Q000648:ultrastructure*
D009124:Muscle Proteins