Methionine transport in Salmonella typhimurium: evidence for at least one low-affinity transport system.
The systems which transport methionine in Salmonella typhimurium LT2 have been studied. Fourteen mutants, isolated by three different selection procedures, had similar growth characteristics and defects in the specific transport process showing a Km of 0.3 microM for L-methionine, and therefore lack the high-affinity, metP transport system. The sites of mutation in four of the mutants were shown by P1-mediated transduction to be linked (0.3 to 1.1%) with a proline marker located at unit 7 on the S. typhimurium chromosome. The high-affinity system was subject to both repression and transinhibition by methionine, and it may also be regulated by the metJ and metK genes. There appeared to be at least two additional transport systems with relatively low affinities for methionine in the metP763 mutant strain, with apparent Km values for methionine of 24 microM and approximately 1.8 mM. The latter system, with a very low affinity for methionine, was inhibited by leucine. In addition, methionine inhibited leucine transport, suggesting that one of the low-affinity methionine transport systems may actually be a leucine transport system.
D000596:Amino Acids / Q000378:metabolism; D001693:Biological Transport, Active / Q000187:drug effects; D002701:Chloramphenicol / Q000494:pharmacology; D005809:Genes, Regulator; D005973:Glutamine / Q000378:metabolism; D007930:Leucine / Q000378:metabolism; D008715:Methionine / Q000378:metabolism*; D009154:Mutation; D012486:Salmonella typhimurium / Q000235:genetics / Q000378:metabolism*; D013237:Stereoisomerism; D014161:Transduction, Genetic; D014364:Tryptophan / Q000378:metabolism
D000596:Amino Acids; D005973:Glutamine; D002701:Chloramphenicol; D014364:Tryptophan; D008715:Methionine; D007930:Leucine