IgM rheumatoid factor as a source of non-specificity in murine anti-allotype sera.
Anti-allotype sera have shown a breakdown of the expected specificity especially when used for the development of haemolysis of fragile target erythrocytes. IgM anti-IgG rheumatoid factor has been shown to be the source of non-specificity. Removal or destruction of IgM in an anti-allotype serum restores the expected specificity.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000276:immunology*; D000918:Antibody Specificity; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D006462:Hemolytic Plaque Technique; D007126:Immunoglobulin Allotypes; D007074:Immunoglobulin G / Q000276:immunology; D007075:Immunoglobulin M / Q000276:immunology*; D007525:Isoelectric Focusing; D051379:Mice; D012217:Rheumatoid Factor / Q000302:isolation & purification
D000888:Antibodies, Anti-Idiotypic; D007126:Immunoglobulin Allotypes; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D012217:Rheumatoid Factor