A combined testing protocol approach for mutagenicity testing.
The antischistosomal agent, hycanthone methanesulfonate (HMS), was employed to illustrate the utility of carrying out several mutagenicity tests in a single concurrent animal experiment. Several commonly used procedures that were successfully integrated into a multiple testing protocol included (1) metaphase analysis in bone marrow, (2) micronucleus test in bone marrow, (3) analysis of the urine for mutagenic constituents, and (4) the host-mediated assay using Salmonella typhimurium. In addition to these animal studies, in vitro mutagenicity testing with and without activation was carried out using S. typhimurium. HMS produced positive, dose--response effects in in vitro tests, metaphase analysis, micronucleus test, and urine analysis, but not in the host-mediated assay. The results of these integrated techniques suggest that such a protocol may be a benefit to those concerned with mutagenicity testing of chemicals.
D000818:Animals; D002467:Cell Nucleus; D002869:Chromosome Aberrations; D004353:Drug Evaluation, Preclinical; D005260:Female; D005821:Genetic Techniques; D006826:Hycanthone / Q000494:pharmacology*; D051379:Mice; D009153:Mutagens; D012486:Salmonella typhimurium / Q000235:genetics; D013892:Thioxanthenes / Q000494:pharmacology*
D009153:Mutagens; D013892:Thioxanthenes; D006826:Hycanthone