Regulation of glucosamine-6-phosphate deaminase synthesis in yeast.
A basal level of glucosamine-6-phosphate deaminase is detected in yeast cells grown on glucose. However, a burst of enzyme production occurs in the presence of N-acetylglucosamine in pathogenic Candida albicans and non-pathogenic Saccharomyces cervisiae. The enzyme synthesis stops and its concentration in the cells declines rapidly as soon as N-acetylglucosamine is removed from the medium. Experiments with RNA- and protein-synthesis inhibitors indicate that the appearance of new enzyme activity is dependent on concomitant new protein synthesis and the inducer operates at a transcriptional level. However, inhibition of DNA synthesis either by hydroxyurea or by mitomycin-C does not impair the synthesis of glucosamine-6-phosphate deaminase.
D000117:Acetylglucosamine / Q000031:analogs & derivatives / Q000378:metabolism; D019747:Aldose-Ketose Isomerases; D002176:Candida albicans / Q000378:metabolism*; D002238:Carbohydrate Epimerases / Q000378:metabolism*; D003513:Cycloheximide / Q000494:pharmacology; D003839:Deoxyadenosines / Q000494:pharmacology; D004996:Ethidium / Q000494:pharmacology; D005944:Glucosamine / Q000031:analogs & derivatives; D005947:Glucose / Q000378:metabolism; D006918:Hydroxyurea / Q000494:pharmacology; D008937:Mitomycins / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
D003839:Deoxyadenosines; D008937:Mitomycins; D003513:Cycloheximide; C020582:glucosamine-6-phosphate isomerase; D002238:Carbohydrate Epimerases; D019747:Aldose-Ketose Isomerases; D004996:Ethidium; D005947:Glucose; D005944:Glucosamine; D000117:Acetylglucosamine; D006918:Hydroxyurea