Comparison of titration results of diphtheric antitoxic antibodies obtained by means of Jensen's method and the methods of tissue cultures and haemagglutination.
In 33 human sera the determination of diphtheric antitoxic antibodies was performed in a double blind test using Jensen's method, the method of tissue cultures and the haemagglutination method. In the method of tissue cultures the antibody levels in the sera were determinated in the first and second experiment with the precision of +/- half dilution of the geometrical progression. In Jensen's method, the difference between the first and second measurements slightly exceeded +/- 1 dilution. In the haemagglutination method the error considerably exceeded the binary step dilution. In most cases, the determination fluctuated up to seven times the actual value. Differences among the mean values of examination results obtained by Jensen's method and the method of the tissue cultures are statistically insignificant. The differences between the haemagglutination method and both the other methods are statistically significant.
D000704:Analysis of Variance; D000907:Antibodies, Bacterial / Q000096:biosynthesis*; D046508:Culture Techniques; D004166:Diphtheria Antitoxin / Q000276:immunology*; D006386:Hemagglutination Tests; D006801:Humans; D007158:Immunologic Techniques; D013223:Statistics as Topic
D000907:Antibodies, Bacterial; D004166:Diphtheria Antitoxin