[Hemagglutinating activity and morphology of antigen K 99 in enterotoxic strains of E. coli isolated from calves].
The hemagglutination activity and morphology of antigen K 99 in enterotoxic E. coli strains isolated from calves was studied. It was proven that antigen K 99 is produced not at 18 degrees C, but at 37 degrees C. All K 99+ E. coli strains react with absorbed anti K 99 serum, showing various anti-agglutination titers. The hemagglutination activity of K 99+ E. coli strains is inhibited by absorbed K 99 antiserum. In immunodiffuse tests all K 99+ E. coli strains react by producing a single precipitation line. It is proven electronmicroscopically that on the surface of the bacterial cell of K 99+ E. coli strains is observed a filamentous structure, consisting of numerous fine fibers which give it a fibriform outlook. No such morphological structures are observed in K 99- E. coli strains.
D000042:Absorption; D000372:Agglutination Tests; D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology* / Q000302:isolation & purification; D002417:Cattle / Q000382:microbiology*; D004926:Escherichia coli / Q000276:immunology* / Q000302:isolation & purification / Q000648:ultrastructure; D006384:Hemagglutination; D006386:Hemagglutination Tests; D008854:Microscopy, Electron
D000942:Antigens, Bacterial
Prouchvaniia vŭrkhu khemaglutinatsionnata aktivnost i morfologiia na antigen K 99 u enterotoksichni shtamove E. coli, izolirani ot teleta.