Mycelial lipids as an aid in identifying rust fungi in culture-Cronartium fusiforme.
The sterol and fatty acid content of mycelium from germinating basidiospores of Cronartium fusiforme was determined. The mycelium contained stigmast-7-enol, fungisterol, and possibly stigmasta-5,7-dienol. No ergosterol was detected. The mycelium contained the expected fatty acids and low relative proportions of 9,10-epoxyoctadecanoic acid. The absence of ergosterol, and presence of the epoxy C18 acid and sterols typical of certain rust spores may be used for a relatively rapid confirmation of rust fungi in culture. Based on these chemical criteria, yeast-like cells isolated from the cultures of germinating basidiospores appear not to be C. fusiforme.
D001487:Basidiomycota / Q000032:analysis* / Q000145:classification / Q000254:growth & development; D005227:Fatty Acids / Q000032:analysis*; D008828:Microbiological Techniques; D013261:Sterols / Q000032:analysis*
D005227:Fatty Acids; D013261:Sterols