title: Origin and direction of replication of the bacteriocinogenic plasmid Clo DF13.
abstract: Cairn's type replicative intermediates of both the wildtype Clo DF13 plasmid and the copy mutant CLO DF13 cop3 were isolated by dye-buoyant density centrifugation. Replicative intermediates were linearized at the HpaI or Sa1I cleavage site, and examined with the electron-microscope. The data show that replication of both the Clo DF13 wild type plasmid and the Clo DF13 cop3 plasmid, initiates at about 2.8% on the physical map. Replication proceeds unindirectionally and counterclockwise on this map.
mesh_terms: D001430:Bacteriocins / Q000096:biosynthesis*; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D008854:Microscopy, Electron; D009690:Nucleic Acid Conformation; D010957:Plasmids
chemical_list: D001430:Bacteriocins; D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes