IgA contamination of IgG prepared on a protein A column.
When protein A is used for the isolation of IgG from pooled human serum further purification steps may be necessary since the IgG is contaminated with approximately 30% of the IgA originally present in the starting material. No preference for binding of an IgA subclass to protein A was found. In addition part of the IgG3 subclass binds to protein A.
D001665:Binding Sites; D005591:Chemical Fractionation; D006801:Humans; D007122:Immunoelectrophoresis; D007070:Immunoglobulin A; D007074:Immunoglobulin G / Q000302:isolation & purification*; D007163:Immunosorbent Techniques; D013205:Staphylococcal Protein A
D007070:Immunoglobulin A; D007074:Immunoglobulin G; D013205:Staphylococcal Protein A