Identification of a high molecular weight actin-binding protein in skeletal muscle.
A large polypeptide having a molecular weight of 240,000 as determined by electrophoresis in the presence of sodium dodecyl sulfate has been identified in whole cell homogenates from chick skeletal muscle myoblasts and the rat myoblast L6 cell line. A similar polypeptide was identified in both thigh and breast chicken skeletal muscle, but the latter contained less of this protein per g of tissue. Antibodies made to gizzard filamin (an actin-binding protein having a molecular weight of 240,000) cross-reacted with the partially purified Mr = 240,000 protein from chicken skeletal muscle. With use of the indirect immunofluorescence technique, the filamin antibody localized in the Z-line region of chicken skeletal muscle myofibrils. These results indicate that skeletal muscle contains a filamin-like protein that may form an integral part of the myofibril structure.
D000199:Actins / Q000378:metabolism*; D000375:Aging; D000818:Animals; D002478:Cells, Cultured; D002645:Chickens; D005455:Fluorescent Antibody Technique; D005895:Gizzard, Avian; D008970:Molecular Weight; D009124:Muscle Proteins / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D009210:Myofibrils / Q000032:analysis; D011485:Protein Binding; D051381:Rats
D000199:Actins; D009124:Muscle Proteins