Radioimmunoassay of LH-RH. Standard curves and specificity.
As important discrepancies in blood concentrations of LH-RH remain, the basic problem of the specificity of the radioimmunoassay was reinvestigated using an antiserum raised against a new conjugate (33). Cross-reactions with related and unrelated peptides were studied in both charcoal-dextran and second antibody methods of separation. The possible relation between the nature of the conjugate and the specificity is discussed.
D000818:Animals; D002854:Chromatography, Paper; D002855:Chromatography, Thin Layer; D003429:Cross Reactions; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000097:blood*; D007106:Immune Sera / Q000276:immunology; D007457:Iodine Radioisotopes; D010455:Peptides / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods* / Q000592:standards; D051381:Rats
D007106:Immune Sera; D007457:Iodine Radioisotopes; D010455:Peptides; D007987:Gonadotropin-Releasing Hormone