Characterization of sterol-ester synthetase in Saccharomyces cerevisiae.
Cell-free extracts of Saccharomyces cerevisiae grown under aerobic as well as semi-anaerobic conditions were found to catalyze the synthesis of fatty acid ester of sterol from cholesterol, fatty acid, ATP and CoA, or from cholesterol and fatty acyl-CoA. This result indicates that the enzyme involved in the formation of the ester is acyl-CoA:sterol O-acyltransferase (EC 2.3.1.26). The enzyme had a broad substrate specificity for sterols and acyl-CoAs. The enzyme levels in the cells grown under aerobic and semi-anaerobic conditions were almost equal. The enzyme was located in the microsomal fraction of the aerobically grown cells.
D000217:Acyltransferases / Q000378:metabolism*; D000332:Aerobiosis; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D002785:Sterol O-Acyltransferase / Q000378:metabolism*; D013347:Subcellular Fractions / Q000201:enzymology; D013379:Substrate Specificity
D000217:Acyltransferases; D002785:Sterol O-Acyltransferase