title: Perfusion fixation of the newborn opossum: equipment and techniques.
abstract: A 30-gauge cannula was used to perfuse fixative through the fragile heart of a newborn opossum. The cannula was gently maneuvered into the heart and held in place with a specially designed manipulator. The flow rate of the fixative was regulated with an infusion set connected to the cannula.
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D005404:Fixatives; D006652:Histological Techniques / Q000662:veterinary*; D009893:Opossums; D010477:Perfusion / Q000295:instrumentation / Q000379:methods / Q000662:veterinary*
chemical_list: D005404:Fixatives