High-performance liquid chromatographic determination of prostacyclin.
A high-performance liquid chromatographic method for the analysis of prostacyclin using a laboratory prepared reversed-phase column packing is described. A relative standard deviation of less than 1% was obtained for ten replicate injections. The system resolves prostacyclin from its hydrolysis product, 6-oxo-prostaglandin F1 alpha and from other prostaglandins present as impurities. These can be estimated to levels of approximately 0.5%. The separation of other unrelated prostaglandins by this method is briefly reported.
D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D011464:Epoprostenol / Q000032:analysis*; D006868:Hydrolysis; D011453:Prostaglandins / Q000032:analysis*
D011453:Prostaglandins; D011464:Epoprostenol