Direct tissue isoelectric focusing in agarose.
We are isoelectric focusing small amounts of tissues placed directly on electroendosmosis-free agarose gels instead of using the saline extracts of tissue homogenates. More numerous proteins are extracted during isoelectric focusing of the solid tissues than present in the same tissues.
D000230:Adenocarcinoma; D003110:Colonic Neoplasms; D006652:Histological Techniques; D006801:Humans; D007525:Isoelectric Focusing / Q000379:methods*; D009363:Neoplasm Proteins / Q000302:isolation & purification; D012685:Sepharose; D013997:Time Factors
D009363:Neoplasm Proteins; D012685:Sepharose