Urid acid accumulated in isolated kidney grafts: an assessment of agonal ischaemia.
Hypoxanthine-xanthine washed out from kidney tissue during preservation has previously been found to be a reliable measure of the in vitro assessment of the deleterious effect of ischaemia on the functional regenerative ability of the graft. We have now studied the question: can uric acid accumulation in the isolated kidney graft be employed as a retrospective measure of the agonal ischaemia? We have found that uric acid alone accumulates in renal tissue during the agonal phase, in an amount that remains unchanged during the subsequent in vitro ischaemia. The determination of hypoxanthine-xanthine and uric acid in kidney perfusate samples during preservation must thus be presumed to be an optimal clinical-chemical method of assessing the graft ischaemia.
D000244:Adenosine Diphosphate / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D005260:Female; D007042:Hypoxanthines / Q000378:metabolism; D066298:In Vitro Techniques; D007288:Inosine / Q000378:metabolism; D007511:Ischemia / Q000378:metabolism*; D007668:Kidney / Q000098:blood supply / Q000378:metabolism; D016030:Kidney Transplantation; D007773:Lactates / Q000378:metabolism; D008297:Male; D009926:Organ Preservation; D011817:Rabbits; D014527:Uric Acid / Q000378:metabolism*; D014970:Xanthines / Q000378:metabolism
D007042:Hypoxanthines; D007773:Lactates; D014970:Xanthines; D014527:Uric Acid; D000249:Adenosine Monophosphate; D007288:Inosine; D000244:Adenosine Diphosphate; D000255:Adenosine Triphosphate