Restriction enzyme cleavage of DNA resulting from gently lysed Escherichia coli.
When E. coli or lambda infected E. coli are gently lysed the DNA is released as a very fast sedimenting species that is presumably bound to membrane material. If this complex is now subjected to restriction enzyme cleavage, only a minor fraction of the fast sedimenting DNA remains and this is found, after purification, to be enriched for branched molecules.
D002462:Cell Membrane / Q000378:metabolism; D003090:Coliphages / Q000235:genetics; D004261:DNA Replication; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004926:Escherichia coli / Q000235:genetics*
D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes