[Reconstruction of native bakers' yeast inorganic pyrophosphatase from its subunits].
The subunits of inorganic pyrophosphatase were obtained both in the presence of 4 or 6 M Gu--HCl and in an alkaline medium at pH 10.5. The reassociation of the subunits into an active dimeric form was carried out using dialysis. After reconstruction the enzyme exhibits the same activity as does native pyrophosphatase.
D006146:Guanidines; D046911:Macromolecular Substances; D008970:Molecular Weight; D010710:Phosphates; D011489:Protein Denaturation; D011755:Pyrophosphatases / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology*
D006146:Guanidines; D046911:Macromolecular Substances; D010710:Phosphates; D011755:Pyrophosphatases
Rekonstruktsiia kataliticheski aktivnogo fermenta iz sub'edinits drozhzhevoĭ neorganicheskoĭ pirofosfatazay.