Fine-structure mapping of the acetamidase structural gene and its controlling region in Aspergillus nidulans.
A large number of amdS mutants altered in acetamide utilization have been used to construct a fine-structure map of the amdS locus. The mutagen diepoxyoctane generated most of the deletion strains used for mapping. A minimum of 14 sites within the amdS gene were found. Biochemical analysis of amdS mutants defined the extent of the probable coding region. A new mutant, amd-205, which did not produce detectable inactive gene product, was found to be inseparable by recombination from the "up-promoter" mutation amdI18 and was located outside of the apparent amdS coding region. The cis-dominant mutation, amdI9, was also located at this end of the gene. This work, therefore, provides evidence for the separation of a eukaryotic gene into controlling and structural regions.
D000081:Acetamides; D000581:Amidohydrolases / Q000235:genetics*; D001233:Aspergillus nidulans / Q000235:genetics*; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D005796:Genes; D009154:Mutation
D000081:Acetamides; D000581:Amidohydrolases