Uroporphyrin- and coproporphyrin I-accumulating mutant of Escherichia coli K12.
A new type of haem-deficient mutant was isolated in Escherichia coli K12 by neomycin selection. The mutant was deficient in uroporphyrinogen III cosynthase activity as indicated by the accumulation of uroporphyrin I and coproporphyrin. The mapping of the corresponding hemD gene by P1-mediated transduction showed that the new gene was located between ilv and cya, at min 83 on the chromosomal map of Escherichia coli K12.
D002874:Chromosome Mapping; D003306:Coproporphyrins / Q000096:biosynthesis; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D005796:Genes; D011163:Hydroxymethylbilane Synthase / Q000378:metabolism; D009154:Mutation; D011166:Porphyrins / Q000096:biosynthesis*; D014161:Transduction, Genetic; D014576:Uroporphyrinogen III Synthetase / Q000378:metabolism; D014578:Uroporphyrins / Q000096:biosynthesis
D003306:Coproporphyrins; D011166:Porphyrins; D014578:Uroporphyrins; D011163:Hydroxymethylbilane Synthase; D014576:Uroporphyrinogen III Synthetase