Microtechnique for studies on the role of monocytes in the stimulation of lymphocytes.
A microtiter technique was used to measure lymphocyte stimulation by antigen-pulsed monocytes. Monocytes from human blood were isolated on polyester film disks. The disks were exposed to antigen and incubated together with lymphocytes in the wells of a microtiter plate. The method was technically simple and required no expensive materials.
D000941:Antigens; D006801:Humans; D007158:Immunologic Techniques; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D009000:Monocytes / Q000276:immunology*; D013696:Temperature
D000941:Antigens