Biosynthesis and turnover of outer-membrane proteins in Escherichia coli ML308-225.
Isolated outer membranes and outer-membrane extracts from Escherichia coli ML308-225 in the early-exponential growth phase contain more protein than do corresponding preparations from late-exponential- or stationary-phase bacteria. Isotope-dilution experiments show that this is due to a loss of protein from the membrane during the exponential growth phase. Inhibition of bacterial growth and protein synthesis stabilizes the outer-membrane-protein concentration. Protein synthesis in the absence of bacterial growth results in higher concentrations of protein in the outer membrane.
D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D002701:Chloramphenicol / Q000494:pharmacology; D003593:Cytoplasm / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000302:isolation & purification; D009268:Nalidixic Acid / Q000494:pharmacology
D001426:Bacterial Proteins; D008565:Membrane Proteins; D009268:Nalidixic Acid; D002701:Chloramphenicol