Demonstration of phosphorylcholine-specific IgE B cells in CBA/N mice.
CBA/N mice, which did not make anti-PC IgM or IgG antibody against PC-conjugated T-dependent or T-independent antigens, produced IgE antibody to PC-determinant when they were immunized with PC-KLH. PC-specificity of IgE antibody produced in CBA/N mice was determined by inhibition of PCA reaction with free PC-hapten or C-polysaccharide or by absorption of reaginic activity in the serum with C-polysaccharide. The presence of T15 idiotype on anti-PC IgE antibody produced in CBA/N x BALB/c F1 males also showed that anti-PC IgE antibody in defective mice was PC-specific. The results suggest that PC-specific B epsilon cells may belong to a subpopulation distinct from PC-specific precursors for IgM and IgG responses.
D000818:Animals; D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000276:immunology*; D002417:Cattle; D002794:Choline / Q000031:analogs & derivatives*; D006433:Hemocyanins / Q000276:immunology; D006462:Hemolytic Plaque Technique; D007073:Immunoglobulin E / Q000096:biosynthesis / Q000276:immunology*; D007075:Immunoglobulin M / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D010323:Passive Cutaneous Anaphylaxis; D010767:Phosphorylcholine / Q000276:immunology*; D012710:Serum Albumin, Bovine / Q000276:immunology
D007075:Immunoglobulin M; D010767:Phosphorylcholine; D012710:Serum Albumin, Bovine; D007073:Immunoglobulin E; D006433:Hemocyanins; D002794:Choline