Inhibition of deoxyribonucleic acid repair in Escherichia coli by caffeine and acriflavine after ultraviolet irradiation.
The effects of caffeine and acriflavine on cell survival, single-strand deoxyribonucleic acid break formation, and postreplication repair in Escherichia coli wild-type WP2 and WP2 uvrA strains after ultraviolet irradiation was studied. Caffeine (0.5 mg/ml) added before and immediately after ultraviolet irradiation inhibited single-strand deoxyribonucleic acid breakage in wild-type WP2 cells. Single-strand breaks, once formed, were no longer subject to repair inhibition by caffeine. At 0.5 to 2 mg/ml, caffeine did not affect postreplication repair in uvrA strains. These data are consistent with the survival data of both irradiated WP2 and uvrA strains in the presence and absence of caffeine. In unirradiated WP2 and uvrA strains, however, a high caffeine concentration (greater than 2 mg/ml) resulted in gradual reduction of colony-forming units. At a concentration insufficient to alter survival of unirradiated cells, acriflavine (2 microgram/ml) inhibited both single-strand deoxyribonucleic acid breakage and postreplication repair after ultraviolet irradiation. These data suggest that although the modes of action for both caffeine and acriflavine may be similar in the inhibition of single-strand deoxyribonucleic acid break formation, they differ in their mechanisms of action on postreplication repair.
D000166:Acridines / Q000494:pharmacology*; D000167:Acriflavine / Q000494:pharmacology*; D002110:Caffeine / Q000494:pharmacology*; D004260:DNA Repair / Q000187:drug effects*; D004269:DNA, Bacterial / Q000378:metabolism* / Q000528:radiation effects; D004926:Escherichia coli / Q000187:drug effects* / Q000528:radiation effects; D011740:Pyrimidine Dimers / Q000378:metabolism; D014466:Ultraviolet Rays
D000166:Acridines; D004269:DNA, Bacterial; D011740:Pyrimidine Dimers; D000167:Acriflavine; D002110:Caffeine