Separation of mouse T cell subsets by a fluorescent activated cell sorter using fluorescence-labeled peanut agglutinin.
Nylon non-adherent spleen T cells obtained from concanavalin A-injected mice were labeled with fluorescein-labeled peanut agglutinin and separated into peanut agglutinin-positive (PNA+) cells and peanut agglutinin-negative (PNA-) cells by a fluorescent activated cell sorter. PNA+ cells were found to exert marked suppressive effect on primary anti-sheep red blood cells antibody response, but PNA- cells did not affect the antibody response. From these results and from the sugar-binding specificity of PNA, suppressor T cells are supposed to possess abundant galactosyl residues exposed on the cell surface, which are not masked by sialyl residues.
D000818:Animals; D010367:Arachis; D001665:Binding Sites; D002469:Cell Separation; D003208:Concanavalin A / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D005690:Galactose / Q000502:physiology; D006462:Hemolytic Plaque Technique; D037102:Lectins; D008213:Lymphocyte Activation; D008211:Lymphocyte Cooperation; D008297:Male; D051379:Mice; D037121:Plant Lectins; D013601:T-Lymphocytes / Q000145:classification*
D037102:Lectins; D037121:Plant Lectins; D003208:Concanavalin A; D005690:Galactose