Measurement by Quantimet 720 of the proportion of actively growing cells in tissue cultures of human lymphocytes.
The size-distribution of normal human lymphocytes growing in tissue culture was measured with a Quantimet 720 image-analysing computer. The proportion of cells undergoing volumetric growth after phytohaemagglutinin (PHA) stimulation was dervived from a simple mathematical model describing the growth patterns of the cells up to the first mitotic division. The approach may have general application in biology as a method for measuring the proportion of growing cells by microscopy.
D002452:Cell Count; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D003201:Computers; D046508:Culture Techniques; D003584:Cytological Techniques; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000166:cytology* / Q000187:drug effects; D008954:Models, Biological; D010835:Phytohemagglutinins / Q000494:pharmacology
D010835:Phytohemagglutinins