title: Activity changes in lac repressor with cysteine oxidation.
abstract: The effects of prior covalent cysteine modification or nonspecific DNA presence on the reaction of lac repressor protein with N-bromosuccinimide have been investigated. At low excesses, N-bromosuccinimide oxidation causes loss of operator DNA binding activity with simultaneous retention of inducer and nonspecific DNA binding activities. Cysteine and methionine are oxidized under the conditions utilized. Covalent modification of the cysteines of repressor prior to reaction decreased the observed loss of operator DNA binding capacity; the presence of nonspecific DNA partially prevented oxidation of the cysteines by N-bromosuccinimide, and concurrent protection of operator binding ability was observed. Methionine oxidation was observed in the cases where protection of the operator DNA binding capacity of repressor was seen. The region surrounding cysteine 107 was found to be influential in maintaining intact operator DNA binding function in repressor. This observation provides chemical evidence for the contribution of the core region of repressor in determining specificity of the protein in binding the lac operator. The protection from oxidation of cysteine residues in the core region by the presence of nonspecific DNA suggests that this binding influences the core region of the protein.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D001974:Bromosuccinimide; D003545:Cysteine; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000032:analysis; D005033:Ethylmaleimide; D007700:Kinetics; D010084:Oxidation-Reduction; D011485:Protein Binding; D012097:Repressor Proteins / Q000302:isolation & purification; D014157:Transcription Factors / Q000302:isolation & purification
chemical_list: D000596:Amino Acids; D004269:DNA, Bacterial; D012097:Repressor Proteins; D014157:Transcription Factors; D003545:Cysteine; D001974:Bromosuccinimide; D005033:Ethylmaleimide