Fluorescent colloidal gold: a cytochemical marker for fluorescent and electron microscopy.
The gold method was further developed for fluorescent microscopy. Gold granules (12 nm in size) were labelled with rhodamine conjugates of Concanavalin A and avidin. The fluorescent markers were used to mark cell wall mannan on the yeast Saccharomyces cerevisiae either by the one-step, or by the two-step method via a biotinyl derivative of ConA. By fluorescence or transmission electron microscopy, the two-step method was found to achieve a higher density of marking.
D001360:Avidin; D003102:Colloids; D003208:Concanavalin A; D005453:Fluorescence; D006046:Gold; D006651:Histocytochemistry / Q000379:methods*; D008854:Microscopy, Electron / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D012441:Saccharomyces cerevisiae / Q000648:ultrastructure
D003102:Colloids; D003208:Concanavalin A; D001360:Avidin; D006046:Gold