A highly sensitive peroxidase-antiperoxidase method for detection of H-Y antigen on cultivated human fibroblasts.
A peroxidase-anti-perioxidase method for the detection of H-Y antigen at the single cell level is described. The efficiency of the test was examined in cultivated fibroblasts derived from control subjects and from XX males and a true hermaphrodite. For comparison, H-Y antigen was determined in blood cells of the same probands using the cytotoxic test. The finding of H-Y positive fibroblasts in the intersex patients has implications for the origin of these disorders.
D002478:Cells, Cultured; D012734:Disorders of Sex Development / Q000235:genetics; D005260:Female; D005347:Fibroblasts / Q000032:analysis; D006182:H-Y Antigen / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male
D006182:H-Y Antigen