Methanol formation in vivo from methylated chemotaxis proteins in Escherichia coli.
Chemotactically wild type Escherichia coli were incubated with L-[methyl-3H]methionine to label the methyl groups of their methyl-accepting chemotaxis proteins. Cells were then treated to specifically demethylate these proteins. We have identified the end product of this demethylation as [3H]methanol in the cell-free medium from treated cells.
D001426:Bacterial Proteins / Q000378:metabolism*; D002474:Cell-Free System; D002633:Chemotaxis; D003080:Cold Temperature; D003640:Dealkylation; D004926:Escherichia coli / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D000432:Methanol / Q000096:biosynthesis*; D008715:Methionine / Q000378:metabolism; D008745:Methylation; D013045:Species Specificity
D001426:Bacterial Proteins; D008565:Membrane Proteins; D008715:Methionine; D000432:Methanol