Separation of human cells bearing HLA-DR antigens using a monoclonal antibody rosetting method.
A technique is described for enriching, from human blood, cells bearing HLA-DR antigens. The method depends on the use of monoclonal mouse antibody which reacts with HLA-DR structures. Cells to which this antibody has bound can be separated after rosetting with bovine erythrocytes coated with anti-mouse immunoglobulin. The cells thus enriched may be used for HLA-DR typing by standard cytotoxicity methods with allogeneic sera.
D000888:Antibodies, Anti-Idiotypic; D002469:Cell Separation / Q000379:methods*; D002499:Centrifugation, Density Gradient; D003470:Culture Media; D005455:Fluorescent Antibody Technique; D006680:HLA Antigens; D006801:Humans; D007106:Immune Sera; D007136:Immunoglobulins; D008212:Lymphocyte Depletion; D008214:Lymphocytes / Q000276:immunology*; D012397:Rosette Formation / Q000379:methods*
D000888:Antibodies, Anti-Idiotypic; D003470:Culture Media; D006680:HLA Antigens; D007106:Immune Sera; D007136:Immunoglobulins