title: Plasmid replication functions. IV. Promoters in the replication region of plasmid R6-5.
abstract: Eight RNA polymerase binding sites have been shown to map within the EcoRI fragment E-2 (replication region RepA EcoRI fragment) of plasmid R6-5 and all but one have been shown to contain active promoters of transcription. Three of the identified promoters are located within a 2.7 kb region essential for controlled, autonomous plasmid replication and may be involved in the functional expression of the three R6-5 replication determinants that have thus far been identified, namely the origin of vegetative replication, oriV, the replication control gene, cop, and the determinant of an essential, positive-acting element, designated RepA.
mesh_terms: D001482:Base Composition; D001665:Binding Sites; D004261:DNA Replication; D004262:DNA Restriction Enzymes; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D009693:Nucleic Acid Hybridization; D010957:Plasmids; D011485:Protein Binding; D014158:Transcription, Genetic
chemical_list: D012321:DNA-Directed RNA Polymerases; D004262:DNA Restriction Enzymes