title: Imaging in flow.
abstract: Imaging in flow has been valuable in investigating discrepancies in flow cell measurements due to cell orientation and flow dynamics. This paper discusses optical consideration in flow imaging, slit and full field imaging systems and various cell motion arresting techniques from the standpoint of image plane exposure and suitable detector choices. It concludes with an explanation of the slit-imaging techniques employed in a multidimensional slit-scan flow system and slit-scan correlation system.
mesh_terms: D002467:Cell Nucleus; D002477:Cells; D003201:Computers; D003584:Cytological Techniques; D005453:Fluorescence; D010783:Photometry