title: Recognition of tRNA Trp by initiation factors from Escherichia coli.
abstract: Binding of acetyl or formyltryptophanyl-tRNA Trp from Escherichia coli or beef liver to E. coli ribosomes is strongly stimulated by E. coli initiation factors and requires GTP. The N-acylated tryptophan is puromycin reactive. Polypeptide chain initiation with acetyltryptophan dependent on poly(U,G) has been demonstrated and is highly dependent on added initiation factors. tRNA Trp appears, therefore, to share some structural features with tRNAfMet of significance to the process of polypeptide chain initiation.
mesh_terms: D004926:Escherichia coli / Q000378:metabolism*; D006160:Guanosine Triphosphate / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D010442:Peptide Chain Initiation, Translational; D010448:Peptide Initiation Factors / Q000378:metabolism*; D011485:Protein Binding; D011691:Puromycin; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism; D014364:Tryptophan / Q000031:analogs & derivatives
chemical_list: D010448:Peptide Initiation Factors; D012346:RNA, Transfer, Amino Acyl; D011691:Puromycin; D006160:Guanosine Triphosphate; D014364:Tryptophan