The influence of ribonucleoside triphosphates, and other factors, on the formation of very-salt-stable RNA-polymerase . su+III-tRNA(tRNATyr)-promoter complexes.
The formation of a stable RNA-polymerase . su+III-tRNA-promoter complex was found to require sigma factor and the incorporation of ribonucleoside triphosphates which match the 5' sequence of the su+III tRNA transcript. This complex, stable to at least 2 M KCl, can be retained on a Millipore filter. Its formation closely parallels the extent of transcription obtained from the su+III tRNA promoter in response both to increasing ionic strength and to temperature during incubation of RNA polymerase with the DNA. The RNA-polymerase . DNA complex retained during this assay therefore appears to relate directly to that formed during promoter-directed transcription. The formation of RNA-polymerase . su+III-tRNA-promoter complexes is sensitive to the presence of ppGpp.
D001483:Base Sequence; D004247:DNA / Q000032:analysis; D012321:DNA-Directed RNA Polymerases; D004355:Drug Stability; D004926:Escherichia coli / Q000201:enzymology; D005374:Filtration; D006153:Guanosine Diphosphate / Q000031:analogs & derivatives; D009994:Osmolar Concentration; D011189:Potassium Chloride; D012343:RNA, Transfer / Q000032:analysis; D012265:Ribonucleotides; D013696:Temperature; D014158:Transcription, Genetic
D012265:Ribonucleotides; D006153:Guanosine Diphosphate; D011189:Potassium Chloride; D004247:DNA; D012343:RNA, Transfer; D012321:DNA-Directed RNA Polymerases