title: Comparison of in vivo and in vitro capability of complement fixation by pemphigus antibodies.
abstract: Using complement immunofluorescence, the capability of complement fixation by bound antibodies in pemphigus lesional skin was compared with circulating intercellular antibodies of complement-fixing type in the same patient. Five lesional skin samples from 6 patients, containing bound IgG, gave positive binding of complement (C3) supplied from fresh guinea pig serum, while only one serum sample showed complement-fixing pemphigus antibodies in the circulation. These results support the view that complement-fixing pemphigus antibodies are rapidly localized to the skin and play a role on the production of acantholysis.
mesh_terms: D000906:Antibodies / Q000032:analysis; D003176:Complement C3 / Q000032:analysis; D003168:Complement Fixation Tests; D005455:Fluorescent Antibody Technique; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D010392:Pemphigus / Q000276:immunology*; D012867:Skin / Q000276:immunology
chemical_list: D000906:Antibodies; D003176:Complement C3; D007074:Immunoglobulin G