title: Laser Raman evidence for new cloverleaf secondary structures for eukaryotic 5.8S RNA and prokaryotic 5S RNA.
abstract: Neither of the two previously proposed secondary structures for eukaryotic 5.8S RNA is consistent with the present laser Raman results. A new, highly stable "cloverleaf" secondary structure not only fits the Raman data but also accounts for previously determined enzymatic partial cleavage patterns, base sequence and pairing homologies, and G-C and A-U base pair numbers and ratios. The new cloverleaf model also conserves several structural features (constant loops, bulges, and stems) consistent with known 5.8S RNA functions. Finally, we propose a similar new cloverleaf secondary structure for Escherichia coli 5S RNA, consonant with many known properties of prokaryotic 5S RNA.
mesh_terms: D055598:Chemical Phenomena; D002621:Chemistry; D004926:Escherichia coli / Q000235:genetics; D005057:Eukaryotic Cells; D008958:Models, Molecular; D008961:Models, Structural; D011387:Prokaryotic Cells; D012313:RNA; D013059:Spectrum Analysis, Raman
chemical_list: D012313:RNA