Uncovered skin grafts in mice.
A technique for suturing ear skin on the lateral thoracic area of recipient mice is described. Using this technique the graft was visible from the day of grafting until rejection was complete and mice were not burdened by circumferential body dressings. Consistent mean survival times were observed in a donor-recipient combination over 1 year's time and technical failures could be recognized. This technique was useful to accurately assess accelerated rejection responses in normal mice that occur 4-8 days after grafting as a result of passively transferred effector cells.
D000818:Animals; D003602:Cytotoxicity, Immunologic; D004423:Ear / Q000637:transplantation; D005260:Female; D006084:Graft Rejection; D006085:Graft Survival; D007116:Immunization, Passive; D007158:Immunologic Techniques; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009026:Mortality; D016038:Skin Transplantation; D013997:Time Factors; D014184:Transplantation, Homologous