The influence of oxygen on the survival and yield of DNA double-strand breaks in irradiated yeast cells.
Survival and induction of DNA double-strand breaks were studied in cells of Saccharomyces cerevisiae irradiated under oxic or anoxic conditions with 30 MeV electrons. A linear relationship between DNA double-strand breakage and dose was found in both cases. The o.e.r.-value for colony forming ability was found to be 1.9 +/- 0.2, whereas the o.e.r.-value for DNA double-strand breakage was 3.0 +/- 0.1. These results are not inconsistent with the idea that DNA double-strand breaks are involved in killing of yeast cells. The frequency of induction of DNA double-strand breaks was found to be 0.74 x 10(-11) double-strand breaks per g/mol per Gy when cells were irradiated under oxygen and 0.24 x 10(-11) double-strand breaks per g/mol per Gy under nitrogen.
D002470:Cell Survival / Q000528:radiation effects*; D004247:DNA / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D004583:Electrons; D010100:Oxygen; D011836:Radiation Tolerance; D012441:Saccharomyces cerevisiae
D004247:DNA; D010100:Oxygen