Concanvalin-A-binding proteins on the surface of human malignant and normal lymphocytes.
The concanavalin-A-binding cell surface glycoproteins from normal and certain leukaemic human lymphocytes were radiolabelled and then solubilized with detergent, isolated by affinity chromatography on Con A insolubilized on agarose beads, and subsequently analysed by SDS-polyacrylamide gel electrophoresis. Leukaemic T cells from patients with Sezary syndrome were found to express major concanavalin-A-binding glycoproteins on their outer surface similar to those of normal T lymphocytes. Leukaemic B cells from patients with chronic lymphocytic leukaemia expressed Con-A-binding proteins similar to those of B-cell lines. HLA antigens were predominant among the major Con-A-binding proteins on the surface of the normal and the malignant T cells studied. Human Ia-like antigens, HLA antigens, and the cell surface immunoglobulins IgD and IgM represented the major Con-A-binding proteins on the B cells studied. beta 2-microglobulin was found associated with HLA antigens on both leukaemic and non-leukaemic T and B cells. The presence of additional Con-A-binding proteins expressed on the surface of the different cell types studied is discussed along with some physical characteristics of the human Ia-like antigens isolated.
D001402:B-Lymphocytes / Q000378:metabolism; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006023:Glycoproteins / Q000378:metabolism*; D006680:HLA Antigens; D006801:Humans; D007106:Immune Sera; D007072:Immunoglobulin D / Q000378:metabolism; D007075:Immunoglobulin M / Q000378:metabolism; D007945:Leukemia, Lymphoid / Q000378:metabolism; D008214:Lymphocytes / Q000378:metabolism*; D011485:Protein Binding; D011947:Receptors, Antigen, B-Cell / Q000378:metabolism; D011952:Receptors, Concanavalin A / Q000378:metabolism*; D011955:Receptors, Drug / Q000378:metabolism*; D012751:Sezary Syndrome / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism; D001613:beta 2-Microglobulin / Q000378:metabolism
D006023:Glycoproteins; D006680:HLA Antigens; D007106:Immune Sera; D007072:Immunoglobulin D; D007075:Immunoglobulin M; D011947:Receptors, Antigen, B-Cell; D011952:Receptors, Concanavalin A; D011955:Receptors, Drug; D001613:beta 2-Microglobulin