Preparation of stable erythrocyte target cells suitable for detection of the antibody response to the haptens azobenzenearsonate, azophenyltrimethylammonium and azophenylphosphorylcholine by plaque-forming cell assay.
The preparation of sheep red blood cells (SRBC) which have been sensitized with the haptens azobenzenearsonate, phosphorylcholine and azophenyltrimethylammonium, and are suitable for use in a plaque-forming cell assay, is described. Each hapten is first coupled to a phenolic imidoester (methyl-p-hydroxybenzimidate) and the substituted imidoesters are employed to prepare target SRBC. Details are presented concerning synthesis and purification of the imidoesters, as well as conditions for coupling to SRBC. The hapten-SRBC prepared by this method are very stable and offer significant advantages over other procedures for coupling these haptens to red blood cells.
D000818:Animals; D000917:Antibody Formation; D001391:Azo Compounds / Q000276:immunology*; D002794:Choline / Q000031:analogs & derivatives*; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D006241:Haptens; D006462:Hemolytic Plaque Technique; D051379:Mice; D010767:Phosphorylcholine / Q000031:analogs & derivatives* / Q000276:immunology; D000644:Quaternary Ammonium Compounds / Q000276:immunology*; D010132:p-Azobenzenearsonate / Q000276:immunology*
D001391:Azo Compounds; D006241:Haptens; D000644:Quaternary Ammonium Compounds; D010767:Phosphorylcholine; D010132:p-Azobenzenearsonate; D002794:Choline