Enkephalin- and somatostatin-like immunoreactivities in human adrenal medulla and pheochromocytoma.
By using the Coons indirect immunofluorescence technique, enkephalin-like immunoreactivity with a granular localization was observed in human adrenal medullary gland cells and pheochromocytomas. In two of the tumors and in a few adrenal gland cells, a somatostatin-like peptide could also be identified. Catecholamine cell types were visualized on adjacent sections with antisera to the synthesizing enzymes dopamine-beta-hydroxylase [DBH; dopamine beta-monooxygenase; 3,4-dihydroxyphenylethylamine, ascorbate: oxygen oxidoreductase (beta-hydroxylating), EC 1.14.17.1] and phenylethanolamine-N-methyltransferase (PNMT; noradrenalin N-methyltransferase; S-adenosyl-L-methionine:phenylethanolamine N-methyltransferase, EC 2.1.1.28). In the normal adrenal medulla more DBH- than PNMT-immunoreactive gland cells were observed. In the adrenal pheochromocytoma both DBH- and PNMT-positive cells were seen, whereas the two extra-adrenal tumors contained only DBH. These findings correlated well with plasma catecholamine measurements. Finally, enkephalin immunoreactive fibers and somatostatin immunoreactive cells were observed in a sympathetic ganglion extirpated together with one of the tumors.
D000310:Adrenal Gland Neoplasms / Q000378:metabolism*; D000313:Adrenal Medulla / Q000378:metabolism*; D004723:Endorphins / Q000378:metabolism*; D004745:Enkephalins / Q000276:immunology / Q000378:metabolism*; D004837:Epinephrine / Q000097:blood; D005455:Fluorescent Antibody Technique; D006801:Humans; D009638:Norepinephrine / Q000097:blood; D010673:Pheochromocytoma / Q000378:metabolism*; D013004:Somatostatin / Q000276:immunology / Q000378:metabolism*
D004723:Endorphins; D004745:Enkephalins; D013004:Somatostatin; D009638:Norepinephrine; D004837:Epinephrine