title: Fractionation and structural elucidation of the active components of aurintricarboxylic acid, a potent inhibitor of protein nucleic acid interactions.
abstract: Commercially available, as well as synthetically prepared, samples of aurintricarboxylic acid (a widely employed potent inhibitor of protein nucleic acid interactions) consist mostly of a heterogeneous collection of polymers, as shown by fractionation schemes utilizing both dialysis and ultrafiltration, and by molecular weight measurements. 13C-NMR studies suggest that the polymeric material is of the phenol-formaldehyde type; inhibitory assays that depend on the formation of a protein-nucleic acid complex revealed that potency varied directly with the molecular weight of the polymer. Fractions of molecular weight 400 were essentially inactive.
mesh_terms: D001312:Aurintricarboxylic Acid / Q000032:analysis* / Q000494:pharmacology; D003509:Cyclohexanecarboxylic Acids / Q000032:analysis*; D004247:DNA / Q000378:metabolism; D004926:Escherichia coli; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight; D011072:Poly U / Q000378:metabolism; D011107:Polylysine / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism; D013057:Spectrum Analysis; D013329:Structure-Activity Relationship
chemical_list: D003509:Cyclohexanecarboxylic Acids; D011107:Polylysine; D011072:Poly U; D001312:Aurintricarboxylic Acid; D004247:DNA