[Induction and repair of breaks in DNA chains in vivo due to the imbalance between DNA and protein synthesis].
A sudden induction of the imbalance between the rates of DNA and protein synthesis in the cell (by nalidixic acid or by thymine starvation) results in the stabilization of breaks in DNA chains in vivo. Such "imbalance induced breaks" represent gaps in DNA chains formed with the participation of exonuclease V. Stabilization of the "imbalance induced breaks" is accompanied by DNA degradation and cell death. Restoration of the imbalance between the rates of DNA and protein syntheses by balanced inhibition completely prevents the stabilization of breaks in cells with competent repair systems. Balanced inhibition of intracellular DNA and protein synthesis decreases the rate of repair and permits to see the sequence of induction (stabilization) and disappearance (repair) of breaks in DNA chains in vivo. Repair of breaks occuring on the background of balanced inhition of DNA and protein synthesis decreases the extent of DNA degradation and completely prevents death of E. coli cells with completely functional repair systems.
D001426:Bacterial Proteins / Q000096:biosynthesis*; D004260:DNA Repair; D004261:DNA Replication / Q000187:drug effects; D004269:DNA, Bacterial / Q000096:biosynthesis*; D004926:Escherichia coli / Q000378:metabolism*; D007700:Kinetics; D009268:Nalidixic Acid / Q000494:pharmacology; D014176:Protein Biosynthesis / Q000187:drug effects
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D009268:Nalidixic Acid
Protsessy stabilizatsii i reparatsii metabolicheskikh razryvov tsepeĭ DNK kak rezul'tat izmeneniia balansa v nutrikletochnykh sintezov DNK i belka.