Immunofluorescent serum gliadin antibodies in children with coeliac disease and various malabsorptive disorders. I. Technique, clinical evaluation and diagnostic use of a gliadin antibody assay using pyruvic aldehyde-treated human red cells.
An immunofluorescent gliadin antibody assay is described using pyruvic aldehyde-stabilized human erythrocytes coated with gliadin. Fifty coeliac children all had high serum IgG-antigliadin titres during a normal diet or a challenge with gluten. On a gluten-free diet (30 children), titres were much lower. In patients followed-up for one year on a gluten-free diet, an initial rise in titres was followed by a slow decline. On challenge, IgG-antigliadin titres showed a slow rise or persistence at the same level in most patients. Fifty-two percent of control children with malabosrptive disorders, but without the typical "flat" mucosal lesion on jejunal biopsy, were shown to have positive titres in their sera, as were 6% of normal children and 4% of adult blood donors. The fluorescent antibody technique was compared with methods commonly used to detect wheat-protein antibodies, and was found to be superior to all of them. The immunofluorescent gliadin antibody assay appears to be useful in following-up children with coeliac disease, and in selecting patients for jejunal biopsy, although it does not replace biopsy.
D000906:Antibodies / Q000032:analysis*; D002446:Celiac Disease / Q000178:diet therapy / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D005455:Fluorescent Antibody Technique / Q000379:methods; D005903:Gliadin / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D008286:Malabsorption Syndromes / Q000276:immunology*; D008297:Male; D010940:Plant Proteins / Q000276:immunology*; D011765:Pyruvaldehyde
D000906:Antibodies; D007074:Immunoglobulin G; D010940:Plant Proteins; D011765:Pyruvaldehyde; D005903:Gliadin