A simple procedure for obtaining autoradiographs of G-banded chromosomes.
Chromosomal preparations from cells flash-labeled with 3H-dT were Giemsa-banded following trypsin digestion, allowed to air-dry, and then were coated with a layer of 1% Formvar. The slides were subsequently coated with radiographic emulsion (NTB2) and processed for autoradiography. The resulting chromosomes had distinct G-banding and radiographic labeling patterns. Chemographic grain formation in the emulsion, normally caused by Giemsa stain, was prevented by the film of Formvar, allowing a very low background grain level.
D000818:Animals; D001345:Autoradiography; D001399:Azure Stains; D002871:Chromosome Banding; D002875:Chromosomes; D003584:Cytological Techniques; D051379:Mice
D001399:Azure Stains