title: Denaturation map of the ColE1-Km plasmid pCR11.
abstract: The denaturation map of EcoRI-digested pCR11, a ColE1-Km plasmid, is described. The 2.0 kilobase ColE1-derived segment contains an adenine+thymine rich site in the colicin immunity gene region. In the 7.2 kilobase kanamycin resistance region, the transposon Tn903 consists of an adenine+thymine rich 0.98 kilobase kan gene region flanked by a guanine+cytosine rich 1.09 kilobase inverted duplication.
mesh_terms: D003086:Bacteriocin Plasmids; D001483:Base Sequence; D002874:Chromosome Mapping; D004269:DNA, Bacterial; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000235:genetics*; D007612:Kanamycin / Q000494:pharmacology; D009691:Nucleic Acid Denaturation; D010957:Plasmids; D011815:R Factors
chemical_list: D004269:DNA, Bacterial; D007612:Kanamycin