Isolation of a thiamine-binding protein from Saccharomyces cerevisiae.
Thiamine-binding protein was isolated from Saccharomyces cerevisiae by successive procedures of cold osmotic shock treatment, DEAE-cellulose chromatography and ultrafiltration. The purified thiamine-binding protein was an electrophoretically homogeneous molecule which appeared to be a glycoprotein with a molecular weight of 140 000 by sodium dodecyl sulfate polyacrylamide gel electrophoresis. No thiamine-binding protein was observed by disc gel electrophoresis in the shock fluid released from yeast cells grown in the presence of 1 muM thiamine, indicating that the formation of this protein is regulated by exogenous thiamine as previously suggested.
D002352:Carrier Proteins / Q000302:isolation & purification* / Q000378:metabolism; D008970:Molecular Weight; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013831:Thiamine / Q000378:metabolism*
D002352:Carrier Proteins; D013831:Thiamine