A novel technique for high resolution analysis of the cytoskeleton.
A new technique is described for producing and examining carbon-platinum replicas of the cytoskeleton of cultured cells. This technique allows the individual components of the cytoskeleton to be visualized and positively identified in the transmission electron microscope, and also permits an analysis of the 3-dimensional disposition of these components.
D000199:Actins; D002244:Carbon; D002462:Cell Membrane / Q000648:ultrastructure*; D003902:Detergents; D008565:Membrane Proteins; D008854:Microscopy, Electron / Q000379:methods*; D015879:Myosin Subfragments; D010984:Platinum
D000199:Actins; D003902:Detergents; D008565:Membrane Proteins; D015879:Myosin Subfragments; D010984:Platinum; D002244:Carbon