title: Improved technique for human leukocyte cultures.
abstract: An improved technique for cultivation of peripheral human lymphocytes is presented. The setting of the blood cells is replaced by a wash in Ficoll to remove the polymorphonucleocytes.
mesh_terms: D002478:Cells, Cultured; D002498:Centrifugation; D003584:Cytological Techniques; D005362:Ficoll; D006801:Humans; D008214:Lymphocytes
chemical_list: D005362:Ficoll