[Studies on staphylococcal enterotoxin B. III. Purification with absorbents].
The biological and immunological activities of Staphylococcal enterotoxin B are stable in pH 2.0 approximately 11.0, and also resistant to proteolytic enzyme (trypsin, pepsin) digestion for 3 approximate 4 hours. Therefore, the toxin could be purified with trypsin digest and then absorbed on kaolin and Kieselgel, followed by eluting the different pH buffer solutions. Further purifications was chromatographied on CM-Sephadex column.
D000042:Absorption; D002852:Chromatography, Ion Exchange; D004768:Enterotoxins / Q000302:isolation & purification*; D007616:Kaolin; D010434:Pepsin A; D013211:Staphylococcus aureus / Q000032:analysis*; D014357:Trypsin
D004768:Enterotoxins; D007616:Kaolin; D014357:Trypsin; D010434:Pepsin A