title: [A new culture medium for growth and sporulation of Entomophthorales species, pathogens of aphids (author's transl)].
abstract: No one of the 3 specific egg yolk fractions (neutral lipids, polar lipids and proteins) was necessary for growth and sporulation of species such as Entomophthora aphidis, E. phalloides and E. thaxteriana, normally grown on egg yolk media. Egg yolk could be replaced by a sunflower oil-yeast extract mixture. E. thaxteriana was especially well studied. Various animal and vegetable fats and even chemically pure triglycerides could replace sunflower oil as a carbon source. Yeast extract was the best nitrogen source tested. In liquid culture, sporulation was directly dependent on yeast extract concentration. When yeast extract concentration was held constant, an increase in oil concentration did not produce an increase in sporulation. High dextrose concentrations inhibited resting spore formation. Liquid cultures of E. thaxteriana regularly produced 3 x 10(6) azygospores/ml in a 20 l fermentor.
mesh_terms: D000818:Animals; D001042:Aphids / Q000382:microbiology*; D003470:Culture Media / Q000494:pharmacology; D004773:Entomophthora / Q000254:growth & development* / Q000472:pathogenicity / Q000502:physiology; D005658:Fungi / Q000254:growth & development*; D009821:Oils / Q000494:pharmacology; D012441:Saccharomyces cerevisiae; D013172:Spores, Fungal / Q000187:drug effects
chemical_list: D003470:Culture Media; D009821:Oils
vernacular_title: Un nouveau milieu pour la croissance et la sporulation d'Entomophthorales pathogènes d'aphides.