Increased amplification of pBR322 plasmid deoxyribonucleic acid in Escherichia coli K-12 strains RR1 and chi1776 grown in the presence of high concentrations of nucleoside.
When pBR322 plasmid-harboring Escherichia coli strains RR1 or chi1776 were grown in the presence of 1 mg of uridine or cytidine per ml and later treated with chloramphenicol, as much as three times more plasmid deoxyribonucleic acid was recovered than would normally be obtained by routine plasmid amplification procedures.
D003470:Culture Media; D003562:Cytidine / Q000494:pharmacology*; D004261:DNA Replication / Q000187:drug effects*; D004269:DNA, Bacterial / Q000096:biosynthesis*; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids; D014529:Uridine / Q000494:pharmacology*
D003470:Culture Media; D004269:DNA, Bacterial; D003562:Cytidine; D014529:Uridine