The use of bactericidal concentrations of antibiotics in hypothermic pulsatile perfusion and the effects on canine renal autograft function.
Although cadaver allograft contamination occurs frequently, the potential danger to the transplant recipient varies. Usually the results of culture from the donor and kidney perfusates are not available at the time of transplantation. A positive culture result often necessitates the use of prophylactic antibiotics. The antibiotic may be potentially nephrotoxic, and in the setting of minimal or changing renal function this effect may be potentiated. If significant contamination with virulent organisms is found prior to transplantation, clinical judgment often dictates that the graft be discarded. Perfusion and storage with solutions containing broad-spectrum antibiotics would substantially reduce the incidence of contamination in perfused kidneys. This would in turn reduce the requirement for potentially nephrotoxic antimicrobial therapy in the transplant recipient. Since the antibiotics tested are effective against a wide range of contaminants and apparently cause no detrimental effects, it would seem reasonable to add them routinely to the perfusate solution.
D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D003404:Creatinine / Q000652:urine; D004285:Dogs; D007668:Kidney / Q000502:physiology; D016030:Kidney Transplantation; D010477:Perfusion; D013530:Surgical Wound Infection / Q000517:prevention & control*; D014182:Transplantation, Autologous
D000900:Anti-Bacterial Agents; D003404:Creatinine