Effect of fixation time on measurement of 2'-deoxyribonucleoside 5'-triphosphate pools in the rat embyro.
Values for the concentrations of deoxyribonucleoside triphosphates in rat embryos on day 12 of gestation, determined by high-pressure liquid chromatography, were artifactually two to three times as high in embryos fixed by cooling in ice/water followed by freezing on solid CO2, in 20s, as in those more rapidly/fixed in liquid N2, in 1 s.
D000818:Animals; D003854:Deoxyribonucleotides / Q000378:metabolism*; D004622:Embryo, Mammalian / Q000378:metabolism*; D005615:Freezing; D006652:Histological Techniques; D051381:Rats; D013997:Time Factors
D003854:Deoxyribonucleotides