A simple procedure for the preparation of large amounts of 50 S ribosomal subunits from Escherichia coli.
D002458:Cell Fractionation / Q000379:methods; D002499:Centrifugation, Density Gradient / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism / Q000648:ultrastructure*; D008970:Molecular Weight; D010458:Peptidyl Transferases / Q000378:metabolism; D012335:RNA, Ribosomal / Q000302:isolation & purification*; D012343:RNA, Transfer / Q000378:metabolism; D012269:Ribosomal Proteins / Q000302:isolation & purification*; D012270:Ribosomes / Q000378:metabolism / Q000648:ultrastructure*
D012335:RNA, Ribosomal; D012269:Ribosomal Proteins; D012343:RNA, Transfer; D010458:Peptidyl Transferases