UV protection and mutagenesis in uvrD, uvrE and recL strains of Escherichia coli carrying the pKM101 plasmid.
The effect of the pKM101 plasmid on UV mutagenesis and survival was examined in DNA-repair-deficient strains of E. coli carrying the uvrD, uvrE and recL mutations. Although enhancement of UV mutagenesis by pKM101 was found in all 3 strains, UV protection was only observed in the uvrD strain. We conclude that the plasmid not only requires lexA+ recA+ functions of the cell, but also those of uvrE+ recL+ for its UV-protective effect.
D004260:DNA Repair; D004926:Escherichia coli / Q000235:genetics / Q000528:radiation effects*; D009154:Mutation; D010641:Phenotype; D010957:Plasmids; D011836:Radiation Tolerance; D014466:Ultraviolet Rays