Properties of interferon induced by purified protein derivative of tuberculin in mice sensitized with BCG or cell-wall skeleton of BCG.
Mice sensitized with either BCG or cell-wall skeleton of BCG (BCG-CWS) produced interferon in blood after stimulation with specific antigen, purified protein derivative of tuberculin (PPD). Both BCG-infected normal (C57BL/6) and thymic nude (BALB/c, nu/nu) mice showed enhanced activity to produce interferon by stimulation with E. coli endotoxin. However, detectable interferon was not produced in athymic nude mice sensitized with BCG or BCG-CWS by stimulation with PPD. Immune-induced interferon (I-IF) produced by BCG-CWS and PPD in mice was different in biological and physicochemical properties from virus-induced interferon. I-IF showed about 100 times more potent L-cell growth inhibitory activity than virus-induced L-cell interferon (L-IF). Both I-IF and L-IF showed macrophage-activating activity, which renders resting macrphages cytotoxic to L1210 leukemia cells. Antiviral and macrophage-activating activity of interferon preparation was not separated physicochemically in this study.
D000818:Animals; D001500:BCG Vaccine / Q000276:immunology*; D003601:Cytotoxicity Tests, Immunologic; D004731:Endotoxins / Q000276:immunology; D004926:Escherichia coli; D005260:Female; D007114:Immunization; D007369:Interferon Inducers / Q000276:immunology*; D007372:Interferons / Q000032:analysis / Q000276:immunology*; D007939:Leukemia L1210 / Q000276:immunology; D008297:Male; D051379:Mice; D008819:Mice, Nude; D014373:Tuberculin / Q000276:immunology*
D001500:BCG Vaccine; D004731:Endotoxins; D007369:Interferon Inducers; D014373:Tuberculin; D007372:Interferons