Identification of protective cell surface proteins in ribosomal fractions from Salmonella typhimurium.
Cell surface antigen preparations from Salmonella typhimurium SR-11 prepared by either trichloroacetic acid extraction or boiling in sodium dodecyl sulfate were able to protect C3H/HeJ, C3H/HeDub, and A/J mice. Some of the proteins found in these preparations were shown to exist in the protective ribosomal fraction isolated from S. typhimurium SR-11. Passage of ribosomes isolated from S. typhimurium SR-11 and 6707 through a Sepharose 2B column removed the protective immunogen from 6707 ribosomes but did not completely remove it from SR-11 ribosomes. Immunity to salmonella infection in C3H/HeJ mice induced by ribosomal vaccines may be dependent on the presence of cell surface proteins in the ribosomal fraction.
D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology*; D000954:Antigens, Surface / Q000276:immunology*; D001426:Bacterial Proteins / Q000276:immunology; D002473:Cell Wall / Q000276:immunology; D008565:Membrane Proteins / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D012270:Ribosomes / Q000032:analysis / Q000276:immunology*; D012481:Salmonella Infections, Animal / Q000276:immunology*; D012486:Salmonella typhimurium / Q000276:immunology* / Q000648:ultrastructure
D000942:Antigens, Bacterial; D000954:Antigens, Surface; D001426:Bacterial Proteins; D008565:Membrane Proteins