Cytochemistry of normal and leukaemic lymphocytes: a review.
Findings with six cytochemical reactions demonstrable in normal and leukaemic lymphocytes were reviewed. The two methods which are presently of greater diagnostic value are the acid phosphatase (AP) and alpha-naphthyl acetate esterase (ANAE) reactions. AP has a definitive role in the diagnosis of acute and chronic T-cell leukaemias, where a strong positive reaction helps to distinguish them from most B-cell lymphoproliferative disorders. New findings concerning the ultrastructural localization of this enzyme are presented. ANAE is of value in distinguishing T-lymphocytes (positive localized reaction) from B lymphocytes (negative reaction) and the T micron from the T gamma subpopulation of T-lymphocytes, a positive reaction demonstrable only in the T micron cells. Other reactions reviewed were PAS, beta-glucoronidase, hexosaminidase and alkaline phosphatase.
D000135:Acid Phosphatase / Q000032:analysis; D000469:Alkaline Phosphatase / Q000032:analysis; D001402:B-Lymphocytes / Q000201:enzymology; D004796:Clinical Enzyme Tests; D005966:Glucuronidase / Q000032:analysis; D006596:Hexosaminidases / Q000032:analysis; D006651:Histocytochemistry; D006801:Humans; D007945:Leukemia, Lymphoid / Q000175:diagnosis* / Q000201:enzymology*; D008214:Lymphocytes / Q000201:enzymology* / Q000648:ultrastructure; D009283:Naphthol AS D Esterase / Q000032:analysis; D010503:Periodic Acid-Schiff Reaction; D013601:T-Lymphocytes / Q000201:enzymology
D009283:Naphthol AS D Esterase; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D006596:Hexosaminidases; D005966:Glucuronidase