A simple method for the inactivation of St. Louis encephalitis virus preparations for immunofluorescent microscopy.
A study was made of different treatments for the inactivation of St. Louis encephalitis virus in smears prepared for immunofluorescence microscopy. Treatment of infected cells with 0.3% betapropiolactone at 56 degrees C for 40 min resulted in an inactivated virus suitable for immunofluorescence studies.
D000096:Acetone / Q000494:pharmacology; D000818:Animals; D004665:Encephalitis Virus, St. Louis / Q000187:drug effects* / Q000472:pathogenicity; D004668:Encephalitis Viruses / Q000187:drug effects*; D005455:Fluorescent Antibody Technique; D005557:Formaldehyde / Q000494:pharmacology; D006358:Hot Temperature; D007783:Lactones / Q000494:pharmacology*; D051379:Mice; D011420:Propiolactone / Q000494:pharmacology*
D007783:Lactones; D000096:Acetone; D005557:Formaldehyde; D011420:Propiolactone