In situ flushing of donor kidneys: its technique and rationale.
In situ flushing of kidneys through an isolated segment of aorta was done on mongrel dogs. The 500 cc cold perfusate at 3 to 4C (delivered in 6 minutes) effectively cooled the kidneys to about 15C. This simplified technique of in situ flushing, its usefulness and rationale are discussed.
D000818:Animals; D003080:Cold Temperature; D004285:Dogs; D005260:Female; D007668:Kidney; D016030:Kidney Transplantation; D008297:Male; D009926:Organ Preservation / Q000379:methods*; D010477:Perfusion / Q000379:methods*; D014021:Tissue Preservation / Q000379:methods*; D014184:Transplantation, Homologous