Donor cornea contamination with McCarey-Kaufman medium preservation.
A patient had corneal infection with Staphylococcus aureus after keratoplasty from tissue preserved in McCarey-Kaufman medium. The small, successfully treated corneal infection was from the same organism cultured from the tissue culture medium at the time of transplantation. Possible sources of donor-tissue contamination included the increase in ocular flora seen in postmortem eyes, blood-borne bacteria, lack of sterile technique in securing donor tissue, inadequate irrigation of the tissue, and inadequate use of topical antibiotics on the tissue. Preservation medium and specimens from medium-preserved and refrigerated corneas should be cultured before and during surgery.
D000287:Administration, Topical; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001414:Bacitracin / Q000008:administration & dosage / Q000627:therapeutic use; D003315:Cornea / Q000382:microbiology; D016039:Corneal Transplantation; D003470:Culture Media / Q000592:standards*; D004340:Drug Contamination; D005260:Female; D005839:Gentamicins / Q000494:pharmacology; D006801:Humans; D007275:Injections, Intravenous; D007634:Keratitis / Q000188:drug therapy / Q000209:etiology*; D008712:Methicillin / Q000008:administration & dosage / Q000627:therapeutic use; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D011183:Postoperative Complications / Q000188:drug therapy / Q000209:etiology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000302:isolation & purification; D014184:Transplantation, Homologous
D000900:Anti-Bacterial Agents; D003470:Culture Media; D005839:Gentamicins; D001414:Bacitracin; D008712:Methicillin