Ingestion of dyed-opsonised yeasts as a simple way of detecting phagocytes in lymphocyte preparations. Cytophilic binding of immunoglobulins by ingesting cells.
Procion-dyed yeasts which have been incubated in fresh serum and washed are readily ingested by human blood monocytes and tumour macrophages during a 30 min incubation period. Uptake is enhanced by centrifugation. Intracellular yeasts can be readily distinquished from extracellular by their much slower uptake of toluidine blue. Yeast ingestion is a much more reliable test for blood monocytes than the latex bead test and it is easier to read. The ingestion test may be combined with a rosette test for surface immunoglobulins (SmIg). Since the yeasts take up immunoglobulins from human serum during the complement-coating stage it is necessary, in a combined ingestion-SmIg test, to use fresh serum from another species (sheep) for opsonisation of the yeasts. A technique is described for reducing the number of immunoglobulin-bearing monocytes to a low level with a combined ingestion-SmIg rosette technique to detect residual immunoglobulin-bearing phagocytes.
D000818:Animals; D001666:Binding Sites, Antibody; D004396:Coloring Agents; D003165:Complement System Proteins / Q000378:metabolism; D006801:Humans; D007136:Immunoglobulins / Q000276:immunology*; D007840:Latex; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008863:Microspheres; D009000:Monocytes / Q000276:immunology; D009504:Neutrophils / Q000276:immunology; D009895:Opsonin Proteins; D010586:Phagocytes / Q000276:immunology*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D012756:Sheep; D014048:Tolonium Chloride
D004396:Coloring Agents; D007136:Immunoglobulins; D007840:Latex; D009895:Opsonin Proteins; D014048:Tolonium Chloride; D003165:Complement System Proteins