A solid-phase enzyme linked immunosorbent assay for the detection of African swine fever virus antigen and antibody.
A solid-phase enzyme-linked immunosorbent assay was developed to measure both African swine fever virus (ASFV) antigen and antibody. Experiments showed it to be reproducible and able to detect limiting antigen concentrations of 50--500 HAD50/ml. The assay was more sensitive than those used at present to detect ASFV antibody and it is suggested that it might be of great diagnostic use in countries where African swine fever has recently appeared.
D000358:African Swine Fever Virus / Q000276:immunology*; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000032:analysis*; D003168:Complement Fixation Tests; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005069:Evaluation Studies as Topic; D007122:Immunoelectrophoresis / Q000379:methods; D007124:Immunoenzyme Techniques / Q000379:methods*; D007497:Iridoviridae / Q000276:immunology*; D013552:Swine / Q000276:immunology
D000914:Antibodies, Viral; D000956:Antigens, Viral