Kinetic properties and regulation by L-ornithine of chicken liver arginase induced by insulin.
After injection of insulin into chickens, a new form of arginase with a 16-fold increase of activity appears in the liver. The new form has a different chromatographic behaviour on DEAE-cellulose. The low activity enzyme has a very high Km value (60mM), and is poorly inhibited by L-ornithine. The induced form of arginase is strongly inhibited by L-ornithine and has an allosteric behaviour which can be described by a Monod-Wyman-Changeux model. 1,4-Diaminobutane, spermine have practically no effect on either form of arginase.
D000818:Animals; D001119:Arginase / Q000378:metabolism*; D002645:Chickens; D004790:Enzyme Induction / Q000187:drug effects; D007328:Insulin / Q000494:pharmacology*; D007700:Kinetics; D008099:Liver / Q000201:enzymology*; D008297:Male; D008345:Manganese / Q000494:pharmacology; D008433:Mathematics; D009952:Ornithine / Q000494:pharmacology*
D007328:Insulin; D008345:Manganese; D009952:Ornithine; D001119:Arginase