[Preparation and characteristics of diploid cell strains from the tissues of a bovine embryo].
Two strains of diploid cells were prepared from the lung (BEL-2) and skin-muscle (SMBE-3) tissues of a bovine embryo. According to the results of morphological and karyological studies, these cultures consisted of fibroblast-like cells retaining diploid chromosome number (60) in the process of long-term cultivation. These cell populations have a limited life span (75-80 passages) and 3 growth phases. The species appurtenance of BEL-2 and SMBE-3 cells was confirmed. The cells were not contaminated with mycoplasma or extraneous viruses, had no tumorigenic activity. The BEL-2 cell culture is most susceptible to measles virus, SMBE-3 culture to vaccinia virus.
D000818:Animals; D002417:Cattle; D002460:Cell Line; D002470:Cell Survival; D002478:Cells, Cultured; D004171:Diploidy; D004622:Embryo, Mammalian / Q000166:cytology*; D005260:Female; D007158:Immunologic Techniques; D008168:Lung / Q000166:cytology; D008459:Measles virus / Q000254:growth & development; D008940:Mitotic Index; D009132:Muscles / Q000166:cytology; D011247:Pregnancy; D012867:Skin / Q000166:cytology; D014616:Vaccinia virus / Q000254:growth & development; D014776:Virus Cultivation
Poluchenie i kharakteristika diploidnykh kletok iz tkanei émbriona korovy.