Cloned fragments of the plasmid ColV,I-K94 specifying virulence and serum resistance.
A cloned BamH1-generated fragment of ColV,I-K94 increased the virulence of Escherichia coli, causing an approximately 100-fold reduction in LD50 for chicks. A genetic determinant for resistance to the bactericidal effects of serum was mapped to a 5,300 base-pair sequence within the fragment. Neither colicin V nor immunity to colicin V affected the pathogenicity of E. coli for chicks.
D000818:Animals; D001770:Blood Bactericidal Activity; D002645:Chickens; D002874:Chromosome Mapping; D003087:Colicins / Q000096:biosynthesis* / Q000235:genetics; D003227:Conjugation, Genetic; D004926:Escherichia coli / Q000235:genetics* / Q000472:pathogenicity; D004927:Escherichia coli Infections; D008040:Genetic Linkage; D010957:Plasmids
D003087:Colicins