An outbreak of hepatitis A investigated by immune electron microscopy and enzyme-linked immunosorbent assay.
In a small outbreak of hepatitis A among members of a hospital staff, excretion of hepatitis A virus could be detected by immune electron microscopy (IEM) and enzyme-linked immunosorbent assay (ELISA) in 3 out of 3 cases tested. Significant increases in antibody titre could be shown in 4 out of 6 cases (IEM), and development of virus specific IgM was shown in all 6 cases (ELISA). Among these patients IgM could be detected after 3 months, while sera drawn after 10 months were negative.
D000914:Antibodies, Viral / Q000032:analysis; D003428:Cross Infection; D004196:Disease Outbreaks; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000382:microbiology; D006506:Hepatitis A / Q000453:epidemiology* / Q000276:immunology / Q000382:microbiology; D006801:Humans; D007158:Immunologic Techniques; D008505:Medical Staff, Hospital
D000914:Antibodies, Viral