Isolation and characterization of preproinsulin mRNA from fetal bovine pancreatic islets.
Total polyadenylated RNA prepared from isolated islets of fetal bovine pancreas was physically characterized. Incubation of this poly A+ RNA with a cell-free protein-synthesizing system derived from wheat germ results in the synthesis of insulin-immunoreactive polypeptide identical in size to that described earlier, 11 200 daltons (Lomedico et al. (1977) J. Biol. Chem. 252, 7971--7978). This material comprised approximately 22% of the total 3H-labeled translation products. Compared to poly A+ RNA from the total pancreas, we conclude that islet mRNA is enriched in proinsulin mRNA.
D000818:Animals; D002417:Cattle; D002474:Cell-Free System; D005333:Fetus / Q000378:metabolism; D007515:Islets of Langerhans / Q000378:metabolism*; D008970:Molecular Weight; D011061:Poly A / Q000378:metabolism; D011384:Proinsulin / Q000096:biosynthesis*; D012333:RNA, Messenger / Q000302:isolation & purification*; D011863:Radioimmunoassay; D012639:Seeds / Q000378:metabolism; D014908:Triticum
D012333:RNA, Messenger; D011061:Poly A; D011384:Proinsulin