An autoradiographic microtechnique to study lymphocyte stimulation in solid cultures.
A microtechnique to study lymphocyte stimulation in solid cultures by autoradiography is described. Using this technique at a concentration of 2 x 10(6) lymphocytes/chamber, it was found that the percentage of proliferating cells was 8.4% with anti-lymphocyte serum, 3.9% with calcium ionophore A23187, 2.5% with phytohemagglutinin P, 5.8% with phytohemagglutinin HA17 and 4.2% with tetradecanoyl-phorbolacetate. Lymphocyte responses were dependent on cell concentration. Lymphocyte responses to phytohemagglutinins P and HA17 were potentiated by incorporation into the cultures of 5% autologous erythrocytes.
D000818:Animals; D000961:Antilymphocyte Serum / Q000494:pharmacology; D001345:Autoradiography / Q000379:methods*; D000001:Calcimycin / Q000494:pharmacology; D002478:Cells, Cultured; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology; D008934:Mitogens; D010835:Phytohemagglutinins / Q000494:pharmacology; D011817:Rabbits
D000961:Antilymphocyte Serum; D008934:Mitogens; D010835:Phytohemagglutinins; D000001:Calcimycin