[Chemical modification of the complex of tRNA Phe with phenylalanyl-tRNA synthetase from Escherichia coli].
Alkylation of E. coli tRNAPhe, bound to the cognate synthetase was investigated. The alkylating reagent is a derivative of 2-chloroethylamine: 2',3'-O-[4(N-2-chloroethyl-N-methylamino)-benzylidene]-uridine-5'-methylphosphate. It was found that the enzyme protects from the reaction D-stem (guanosine G24) and the region of juxtaposition of acceptor stem and D-stem (S4U8 and C13) in the tRNAPhe.
D000478:Alkylation; D000604:Amino Acyl-tRNA Synthetases / Q000378:metabolism; D001483:Base Sequence; D004926:Escherichia coli / Q000201:enzymology*; D009690:Nucleic Acid Conformation; D010649:Phenylalanine; D010652:Phenylalanine-tRNA Ligase / Q000378:metabolism; D011485:Protein Binding; D012343:RNA, Transfer
D010649:Phenylalanine; D012343:RNA, Transfer; D000604:Amino Acyl-tRNA Synthetases; D010652:Phenylalanine-tRNA Ligase
Issledovanie khimicheskoĭ modifikatsii kompleksa tRNK s fenilalanil-tRNK-sintetazoĭ Escherichia coli.