title: Lac UV5 transcription in vitro. Rate limitation subsequent to formation of an RNA polymerase-DNA complex.
abstract: The kinetics of transcription of lac UV5 mRNA using purified DNA restriction fragment as template has been studied. This template, which contains only 203 base pairs, directs the formation of a 67-base lac mRNA with high specificity. The half-time for formation of a DNA-RNA polymerase complex is approximately 0.2 min. However, upon addition of 200 micron nucleoside triphosphates to this complex, RNA production proceeds with a half-time of approximately 1 min. Therefore, it is suggested that the rate-limiting step for lac UV5 mRNA production, under typical in vitro conditions, occurs subsequent to the formation of a promoter-specific complex.
mesh_terms: D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D007700:Kinetics; D007763:Lac Operon; D008274:Magnesium / Q000494:pharmacology; D008970:Molecular Weight; D010957:Plasmids; D011188:Potassium / Q000494:pharmacology; D011485:Protein Binding; D012333:RNA, Messenger / Q000096:biosynthesis*; D013698:Templates, Genetic; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D004269:DNA, Bacterial; D012333:RNA, Messenger; D004262:DNA Restriction Enzymes; D008274:Magnesium; D011188:Potassium