Individual cell sorting.
Current cell sorting machines do not preserve the individual identity of processed cells; after analysis, the cells are assigned to a subpopulation where they are pooled with other similar cells. This paper reports progress on a system that sorts cells individually to precise locations on a microscope slide and preserves them for further observation with a light microscope while recording flow measurement data for each cell. Various electronic and mechanical modifications to an existing sorting machine are described that increase drop placement accuracy and permit individual cell sorting.
D000818:Animals; D002469:Cell Separation; D003584:Cytological Techniques / Q000295:instrumentation; D006801:Humans; D008214:Lymphocytes / Q000166:cytology*; D051379:Mice; D008856:Microscopy, Fluorescence; D008863:Microspheres; D008954:Models, Biological