Identification of ergosta-8,24(28)-dien-3 beta,6 alpha-diol in A delta 8 goes to delta 7 sterol isomerase-blocked yeast mutant.
In addition to the monohydroxysterols found in the delta 8 goes to delta 7 isomerase-blocked Saccharomyces cerevisiae mutant erg 2, a noval dihydroxysterol, ergosta-8,24(38)-dien-3 beta,6 alpha-diol, was isolated. This sterol accumulated to the extent of 2.1% of the total sterol fraction when this mutant was treated with 23-azacholesterol, a known inhibitor of the 24-methylene-sterol-24(28)-reductase.
D002849:Chromatography, Gas; D004875:Ergosterol / Q000031:analogs & derivatives* / Q000032:analysis; D007535:Isomerases / Q000032:analysis*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D013251:Steroid Isomerases / Q000032:analysis*
D007535:Isomerases; D013251:Steroid Isomerases; D004875:Ergosterol