[Use of the immunofluorescent microagglutination reaction for the purpose of studying ornithosis infection. 1. Production and approbation of a corpuscular luminescent diagnosticum].
A method of obtaining the fluorescent ornithosis corpuscular diagnostic agent providing for the extraction of the corpuscular antigen with its subsequent conjugation with fluorochrome-fluoresceine isothiocyanate. The use of this preparation permits to stage the agglutination reaction on the basis of immunofluorescent analysis, this facilitating the recording of the reaction results and considerably decreasing consumption of the diagnostic agent. As shown, the suggested immunofluorescent microagglutination reaction was characterised by a sufficiently high sensitivity, specificity, and can be used for detection of antibodies to the causative agent of ornithosis.
D000372:Agglutination Tests / Q000379:methods*; D000818:Animals; D000942:Antigens, Bacterial / Q000302:isolation & purification*; D002689:Chlamydia / Q000276:immunology; D002691:Chlamydophila psittaci / Q000276:immunology*; D005452:Fluoresceins; D005455:Fluorescent Antibody Technique; D006168:Guinea Pigs; D008163:Luminescent Measurements; D051379:Mice; D009956:Psittacosis / Q000175:diagnosis*; D011817:Rabbits; D012698:Serologic Tests / Q000379:methods
D000942:Antigens, Bacterial; D005452:Fluoresceins
Ispol'zovanie immunofliuorestsentnoĭ reaktsii mikroaggliutinatsii s tsel'iu izucheniia ornitoznoĭ infektsii. Soobshchenie 1. Poluchenie i aprobatsiia korpuskulianogo liuminesstsiruiushchego diagnostikuma.