Escherichia coli K-12 auxotrophs induced by insertion of the transposable element Tn5.
The sites of insertion of the transposable kanamycin-neomycin resistance-determining element, Tn5, in the E. coli K-12 chromosome were assessed in a collection of over 300 auxotrophs. Although mutations in at least 45 different cistrons were obtained, the distribution of insertion sites was not completely random: proA or proB; cysG; and cysH, cysD or cysC mutants were found in excess.
D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial; D004251:DNA Transposable Elements; D004352:Drug Resistance, Microbial; D004926:Escherichia coli / Q000235:genetics*; D007612:Kanamycin / Q000494:pharmacology; D009154:Mutation; D009355:Neomycin / Q000494:pharmacology; D011995:Recombination, Genetic
D004251:DNA Transposable Elements; D007612:Kanamycin; D009355:Neomycin