The vasotoxicity of the lysosomal contents from the renal cortex.
The retained proteins (fraction B: rich in renin) and the not retained proteins (fraction A: no renin activity) were obtained from the concanavalin A affinity column chromatography of the lysosomal contents from the hog renal cortex. Intraperitoneal injection of fraction A, B and highly purified renin produced angionecrosis of fibrinoid type of the small arteries and arterioles in the bilaterally nephrectomized rats. The angionecrosis producing activity (vasotoxicity) as well as vasopressor activity of fraction B were prevented completely by the oral administration of SQ14225. Although fraction A demonstrated no renin activity, intraperitoneal injection of fraction A produced gradual and transient elevation of blood pressure and fibrinoid necrosis which was milder and less consistent as compared with fraction B. The vasotoxicity as well as the transient vasopressor activity of fraction A were completely abolished also by the previous administration of oral SQ14225.
D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D002216:Captopril / Q000494:pharmacology; D007672:Kidney Cortex / Q000502:physiology*; D008247:Lysosomes / Q000502:physiology*; D009131:Muscle, Smooth, Vascular / Q000187:drug effects / Q000473:pathology; D011506:Proteins / Q000302:isolation & purification* / Q000494:pharmacology; D051381:Rats; D012083:Renin / Q000302:isolation & purification / Q000494:pharmacology*; D013552:Swine
D011506:Proteins; D002216:Captopril; D012083:Renin