Isolation of recombinant plasmids bearing cDNA to hen ovomucoid and lysozyme mRNAs.
A large library of hen oviduct cDNA-pCR1 recombinant plasmids has been established in Escherichia coli X1776. From this library, ovomucoid cDNA and lysozyme cDNA-bearing plasmids have been identified. One of these plasmids, pMu7, yielded the sequence of the 3'-untranslated region of ovomucoid mRNA.
D000818:Animals; D002645:Chickens; D004262:DNA Restriction Enzymes; D004274:DNA, Recombinant / Q000302:isolation & purification* / Q000378:metabolism; D004527:Egg Proteins / Q000096:biosynthesis*; D004926:Escherichia coli / Q000378:metabolism; D005260:Female; D009113:Muramidase / Q000096:biosynthesis*; D009693:Nucleic Acid Hybridization; D010057:Oviducts / Q000378:metabolism*; D010059:Ovomucin / Q000096:biosynthesis*; D010957:Plasmids; D012333:RNA, Messenger / Q000378:metabolism*
D004274:DNA, Recombinant; D004527:Egg Proteins; D012333:RNA, Messenger; D010059:Ovomucin; D004262:DNA Restriction Enzymes; D009113:Muramidase