Functional characterization of mouse T lymphocytes with IgM-Fc receptors. I. Studies on ADCC and helper cell function.
The function of mouse T lymphocytes with receptors for IgM-Fc was analyzed by using immunofluorescence and a column separation method. The cellular uptake of FITC-IgM was inhibited with unlabeled Fc5 mu. IgM-FcR+ cells were active in ADCC against IgM-sensitized target cells, but not against IgG-coated target cells. The cells mediating ADCC with IgM antibody were shown to be distinct and physically separable from those mediating ADCC with IgG antibody. Helper T lymphocytes for humoral antibody formation in a hapten-carrier system were shown to be IgM-FcR+ and Fc-IgG-.
D000818:Animals; D000918:Antibody Specificity; D000920:Antibody-Dependent Cell Cytotoxicity; D001666:Binding Sites, Antibody; D002469:Cell Separation; D005455:Fluorescent Antibody Technique; D006801:Humans; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D051379:Mice; D008805:Mice, Inbred A; D008811:Mice, Inbred DBA; D011947:Receptors, Antigen, B-Cell; D011961:Receptors, Fc; D013601:T-Lymphocytes / Q000276:immunology*
D007074:Immunoglobulin G; D007075:Immunoglobulin M; D011947:Receptors, Antigen, B-Cell; D011961:Receptors, Fc