title: Interaction between RNA polymerase and a ribosomal RNA promoter of E. coli.
abstract: The interaction between RNA polymerase and the E. coli ribosomal (r) RNA promoter(s) of the rrnE operon has been studied by the filter-binding method. The extent of complex formation between RNA polymerase and rrnE promoter(s) is salt-dependent; ppGpp specifically inhibits interaction of RNA polymerase with the rrnE promoter(s). A tentative model is proposed for the molecular events in the early steps of rRNA initiation: a transition of the primarily formed, labile RNA polymerase-rRNA promoter complex to a more stable form is the determining step. This step is salt-sensitive; ppGpp acts on this "isomerization".
mesh_terms: D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004355:Drug Stability; D004926:Escherichia coli / Q000378:metabolism*; D006159:Guanosine Tetraphosphate / Q000494:pharmacology; D007700:Kinetics; D009876:Operon; D011485:Protein Binding; D012335:RNA, Ribosomal / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D012335:RNA, Ribosomal; D006159:Guanosine Tetraphosphate; D012321:DNA-Directed RNA Polymerases