Expression of the major red cell sialoglycoprotein, glycophorin A, in the human leukemic cell line K562.
We have found that the human leukemic cell line K562 (Lozzio, C.B., and Lozzio, B.B. (1975) Blood 45, 321-334) synthesizes a surface membrane glycoprotein which is identical or closely similar to the major red cell sialoglycoprotein, glycophorin A. The protein can be precipitated by specific anti-glycophorin A antiserum both from surface-labeled and metabolically labeled K562 cells. Cyanogen bromide cleavage of glycophorin A from red cells and the K562 cell protein gives apparently identical fragments, and the glycopeptides and oligosaccharides obtained after Pronase and mild alkaline treatment are closely similar. An antiserum made against intact K562 cells and absorbed with normal human white blood cells precipitated surface-labeled glycophorin A from erythrocytes. The amount of glycophorin A per cell in erythrocytes and K562 cells was very similar when determined by radioimmunoassay. The K562 cells contained blood group MN activity when tested with rabbit anti-M and anti-N sera. When incubated at 37 degrees C with rabbit anti-glycophorin A F(AB)2 fragments and fluorescent sheep anti-rabbit IgG, partial redistribution of glycophorin A (patching and capping) was seen in K562 cells but not in erythrocytes.
D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D004912:Erythrocytes / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D006020:Glycopeptides / Q000302:isolation & purification; D006021:Glycophorins / Q000096:biosynthesis*; D006801:Humans; D007938:Leukemia / Q000378:metabolism*; D008715:Methionine; D011863:Radioimmunoassay; D012795:Sialoglycoproteins / Q000096:biosynthesis*
D006020:Glycopeptides; D006021:Glycophorins; D012795:Sialoglycoproteins; D008715:Methionine