Effect of R plasmid RPI on the nutritional requirements of Escherichia coli in batch culture.
The minimal nutritional requirements of Escherichia coli have been quantitatively determined in batch culture for cells with (R+) and without (R-) the R plasmid RP1. In these conditions R+ cells have a greater requirement than R- for several nutrients, particularly Mg2+, K+, Fe2+ and PO43-. The maximum growth rate in a simple salts medium was the same for R+ and R- cells. At low concentrations of phosphate, the specific growth rate of R+ cells differed from that found for R- cells. The R plasmid was stable in simple salts medium, irrespective of the nutrient ultimately depleted by growth, but, on storage, R+ cells survived for a shorter time than R- cells.
D003470:Culture Media; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D008274:Magnesium / Q000494:pharmacology; D010710:Phosphates / Q000494:pharmacology; D011188:Potassium / Q000494:pharmacology; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D011815:R Factors / Q000187:drug effects; D013431:Sulfates / Q000494:pharmacology
D003470:Culture Media; D010710:Phosphates; D000644:Quaternary Ammonium Compounds; D013431:Sulfates; D008274:Magnesium; D011188:Potassium