[Determination of human leukocyte interferon by a microfluorimetric immunologic method].
The possibility of quantitative determination of human leucocyte interferon using FITC-labeled antiinterferon antibodies was studied. A highly specific antiinterferon immunoglobulin was obtained as a result of longterm immunization of a donkey with human leucocyte interferon followed by fractionation and immuno-absorbtion of immune plasma. This immunoglobulin was labeled with FITC and used for human leucocyte interferon assay in direct and indirect reactions of fluorescence immunoinhibition. The titres of different human leucocyte interferon preparations in this immunoassay were comparable with the titres of the same preparations detected by interferon inhibition of viral cytopathic effect.
D005455:Fluorescent Antibody Technique; D006801:Humans; D007372:Interferons / Q000032:analysis*; D007962:Leukocytes / Q000276:immunology*
D007372:Interferons
Opredelenie chelovecheskogo leĭkotsitarnogo interferona mikrofliuorimetricheskim immunologicheskim metodom.