title: Instrument sterilization in orthodontic offices.
abstract: 1. Three different quaternary ammonium compound solutions remained bactericidal against specific vegetative bacteria in three orthodontic offices over a ten day working period. However, no spore formers or viruses were tested. 2. Bacterial contaminants were cultured on pliers and scalers at the chair at least once in each of three orthodontic offices sampled twice a day for ten working days. 3. Sampled chairside instruments wiped with an alcohol sponge only, between patients, were contaminated an excessive 32.5% of the time, too frequently to be seriously considered for routine disinfection of pliers. 4. Chairside instruments, sampled regardless of other means of disinfection or sterilization used, were contaminated from 3.5 to 15% of the time. Therefore, storage and handling of orthodontic instruments must be evaluated and upgraded to prevent recontamination of previously sterilized instruments. 5. Staff personnel need courses in sterilization and disinfection procedures to prevent cross contamination from patient to patient and to protect themselves. These courses should be related specifically to orthodontic practice procedures.
mesh_terms: D001419:Bacteria / Q000302:isolation & purification; D003762:Dental Instruments; D004202:Disinfectants; D004926:Escherichia coli / Q000187:drug effects; D006358:Hot Temperature; D009970:Orthodontics / Q000295:instrumentation*; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects; D013242:Sterilization / Q000295:instrumentation / Q000379:methods*
chemical_list: D004202:Disinfectants; D000644:Quaternary Ammonium Compounds