The properties of a bacteriophage T5 mutant unable to induce deoxyuridine 5'-triphosphate nucleotidohydrolase. Synthesis of uracil-containing T5 deoxyribonucleic acid.
Bacteriophage T5 induces a deoxyuridine 5'-triphosphate nucleotidohydrolase (dUTPase) activity during infection of Escherichia coli. A T5 mutant (T5 dut) unable to induce this dUTPase activity has been isolated. Although this mutant is viable, the E. coli dUTPase activity is not sufficiently active to exclude uracil from the progeny DNA and about 3% of the thymine is replaced by uracil. When the mutant is grown in an E. coli dut host about 12% of the thymine in the progeny DNA is replaced by uracil. T5 phage containing 12% uracil can replicate in uracil-DNA glycosylase-deficient (ung) hosts with high efficiency, but fail to replicate in ung+ hosts. The amount of thymine replaced by uracil in the progeny produced in dut hosts is nearly independent of the ung genotype, indicating that the host uracil-DNA glycosylase-dependent repair pathway is not operating efficiently to remove uracil from T5 progeny DNA.
D003090:Coliphages / Q000201:enzymology*; D004279:DNA, Viral / Q000096:biosynthesis*; D003856:Deoxyuracil Nucleotides; D004790:Enzyme Induction; D004926:Escherichia coli / Q000201:enzymology*; D005838:Genotype; D007700:Kinetics; D011755:Pyrophosphatases / Q000096:biosynthesis*; D013045:Species Specificity; D014529:Uridine / Q000378:metabolism; D014779:Virus Replication
D004279:DNA, Viral; D003856:Deoxyuracil Nucleotides; D011755:Pyrophosphatases; C020020:dUTP pyrophosphatase; D014529:Uridine