An enzyme immunoassay (EIA) for progesterone in horse plasma.
A simple enzyme immunoassay (EIA) for the measurement of progesterone is described. Antibody against 11-OH-hemisuccinate-BSA is bound to polystyrene tubes. 11-OH-hemisuccinyl-beta-D-galactosidase is used as enzyme-coupled antigen and methylumbelliferyl-beta-D-galactoside as substrate. Concentrations down to 0.156 ng/ml plasm or amounts of 93 pg/tube are detectable. Probit analysis gave a linear relationship between log concentration and percentage of binding. A comparison of EIA and radioimmunoassay gave a correlation coefficient of 0.81. The assay is sufficiently sensitive to estimate progesterone levels in plasma.
D000818:Animals; D000918:Antibody Specificity; D002417:Cattle; D005260:Female; D005455:Fluorescent Antibody Technique; D006736:Horses; D007124:Immunoenzyme Techniques; D011374:Progesterone / Q000276:immunology*; D011817:Rabbits; D011863:Radioimmunoassay
D011374:Progesterone