Enzyme-linked immunosorbent assay for detection of Clostridium botulinum type E toxin.
The enzyme-linked immunosorbent assay using the "double-sandwich" technique was utilized to determine Clostridium botulinum type E toxin. With this technique, about 80 mouse intraperitoneal 50% lethal doses of toxin could be detected. Cross-reaction was hardly observed with C. botulinum type A and B toxins. No cross-reaction was observed with culture supernatants of C. botulinum type C or other Clostridium strains. In all probability this was due to the high specificity of the antiserum prepared aginst the toxic component of type E toxin.
D000818:Animals; D001905:Botulinum Toxins / Q000032:analysis* / Q000633:toxicity; D003014:Clostridium botulinum / Q000032:analysis; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D007124:Immunoenzyme Techniques; D007928:Lethal Dose 50; D051379:Mice; D013045:Species Specificity
D001905:Botulinum Toxins