title: Purification of pseudouridylate synthetase I from Salmonella typhimurium.
abstract: Pseudouridylate synthetase from Salmonella typhimurium has been purified 1,000 fold and is about 90% pure. The enzyme has a molecular weight of 50,000 daltons. In the presence of tRNA there is a change in molecular weight from 50.000 to 100.000. This change does not seem to be due to the formation of a tRNA-enzyme complex but rather to a tRNA induced dimerization. Other properties of the enzyme are described.
mesh_terms: D000595:Amino Acid Sequence; D000926:Anticodon; D001483:Base Sequence; D006836:Hydro-Lyases; D007460:Iodoacetamide / Q000494:pharmacology; D007700:Kinetics; D046911:Macromolecular Substances; D008623:Mercaptoethanol / Q000494:pharmacology; D008970:Molecular Weight; D010430:Pentosyltransferases / Q000302:isolation & purification* / Q000378:metabolism; D011560:Pseudouridine; D012343:RNA, Transfer / Q000494:pharmacology; D012486:Salmonella typhimurium / Q000201:enzymology*; D014498:Uracil
chemical_list: D000926:Anticodon; D046911:Macromolecular Substances; D011560:Pseudouridine; D014498:Uracil; D008623:Mercaptoethanol; D012343:RNA, Transfer; D010430:Pentosyltransferases; D006836:Hydro-Lyases; C022479:pseudouridylate synthetase; D007460:Iodoacetamide