Dilutions and specificity analysis of pretransplant sera.
Six serial dilutions of 51 sera from pretransplant patients were reacted against T and B lymphocytes at 5 degrees C and 37 degrees C. By the use of defined panels of T and B lymphocytes it could be shown that 10% of the 51 positive sera contained T-warm antibodies against HLA-A, -B, and -C specificities. There were 28% of the sera that had antibodies against non-HLA antigens reactive to B lymphocytes in the cold. Other sera contained mixtures of HLA and non-HLA antibodies. By dilution analysis of the sera, the mixtures could be detected and the HLA specificities identified. This ability to distinguish between HLA and non-HLA antibodies should be important in classifying pretransplant patients into high- and low-risk patients. Prior evidence that we have presented suggests that the non-HLA antibodies may be enhancing antibodies.
D000918:Antibody Specificity; D000954:Antigens, Surface; D000961:Antilymphocyte Serum / Q000032:analysis*; D001402:B-Lymphocytes / Q000276:immunology*; D003080:Cold Temperature; D003602:Cytotoxicity, Immunologic; D006680:HLA Antigens; D006358:Hot Temperature; D006801:Humans; D007518:Isoantibodies / Q000032:analysis*; D016030:Kidney Transplantation; D013601:T-Lymphocytes / Q000276:immunology*; D014184:Transplantation, Homologous
D000954:Antigens, Surface; D000961:Antilymphocyte Serum; D006680:HLA Antigens; D007518:Isoantibodies