Do low doses of deoxycholate modify the release of rat jejunal brush border hydrolases?
The in-vivo effects of sodium deoxycholate (DOC) at low concentrations on the release of protein and some brush border hydrolases, sucrase (SA), maltase (MA), leucine aminopeptidase (LA), alkaline phosphatase (AP), have been investigated in the rat by a jejunal perfusion technique. During perfusion with DOC (0.125 or 0.25 mmol/l), enzyme release was not enhanced. After removal of DOC from the perfusion solution with 0.125 mmol/l DOC, there was a steady release of SA, MA and AP although enzyme release was increased linearly in the control and the 0.25 mmol/l DOC groups. The results also confirm the deep localization of AP within the membrane.
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D003840:Deoxycholic Acid / Q000494:pharmacology*; D006867:Hydrolases / Q000378:metabolism*; D007583:Jejunum / Q000187:drug effects / Q000201:enzymology* / Q000648:ultrastructure; D007931:Leucyl Aminopeptidase / Q000378:metabolism; D008871:Microvilli / Q000187:drug effects / Q000201:enzymology; D051381:Rats; D013393:Sucrase / Q000378:metabolism; D000520:alpha-Glucosidases / Q000378:metabolism
D003840:Deoxycholic Acid; D006867:Hydrolases; D000469:Alkaline Phosphatase; D000520:alpha-Glucosidases; D013393:Sucrase; D007931:Leucyl Aminopeptidase