Specific fluorescent labeling of 7-(aminomethyl)-7-deazaguanosine located in the anticodon of tRNATyr isolated from E. coli mutant.
Under-modified E. coli tRNATyr that contains 7-(aminomethyl)-7-deazaguanosine in place of Q nucleoside can be chemically modified by dansyl chloride under neutral conditions. Fluorescent labelling specifically occurred only in the 7-(aminomethyl)-7-deazaguanine moiety. The modified tRNATyr was found to be active both in aminoacylation and in binding to ribosomes.
D000926:Anticodon / Q000378:metabolism; D003619:Dansyl Compounds; D004926:Escherichia coli / Q000378:metabolism*; D006151:Guanosine / Q000031:analogs & derivatives*; D007700:Kinetics; D012343:RNA, Transfer / Q000378:metabolism; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D014443:Tyrosine
D000926:Anticodon; D003619:Dansyl Compounds; D012346:RNA, Transfer, Amino Acyl; D006151:Guanosine; D014443:Tyrosine; D012343:RNA, Transfer