[Micromethod for rabies antibody detection by immunoenzymatic assay with Staphylococcus protein A (author's transl)].
The rabies virus and its glycoprotein are used as antigens in a microimmunoenzymatic assay (IEA). Human rabies antibodies are detected and titrated using anti-human antibodies and staphylococcus protein A conjugated to peroxidase. Several mammal antirabies sera are tested by this method with protein A. The results are compared to those obtained by seroneutralization in mice, which is the standard method for rabies antibody detection.
D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D000956:Antigens, Viral / Q000276:immunology; D006023:Glycoproteins / Q000276:immunology; D006801:Humans; D007124:Immunoenzyme Techniques; D051379:Mice; D009500:Neutralization Tests; D011820:Rabies virus / Q000276:immunology*; D013205:Staphylococcal Protein A / Q000276:immunology*; D014764:Viral Proteins / Q000276:immunology
D000914:Antibodies, Viral; D000956:Antigens, Viral; D006023:Glycoproteins; D013205:Staphylococcal Protein A; D014764:Viral Proteins
Microméthode immunoenzymatique de titrage des anticorps antirabiques: utilisation de la glycoprotéine rabique et de la protéine A conjuguées à la peroxydase.