[Study of amino monosaccharides by thermoionic detection in gas-liquid chromatography. Application to a pyroglobulin IgM (author's transl)].
The monosaccharides of proteic samples liberated by methanolysis and submitted to trimethylsilylation by the trimethylsilylimidazole (TSIM) are determined by gas-liquid chromatography. The neutral sugars are detected in simple flame ionization (FID) and the amino sugars identified separatively by the use of a thermoionic detector (TID) which responds selectively to nitrogenous and phosphorated compounds. The method described has been tested on standard mixtures of monosaccharides and on a well known glycoprotein : the uromucoid. Then it has been applied to a pyroglobulin IgM (mu2, chi2).
D000606:Amino Sugars / Q000032:analysis*; D002849:Chromatography, Gas / Q000379:methods*; D006023:Glycoproteins / Q000032:analysis; D007075:Immunoglobulin M / Q000032:analysis*; D010266:Paraproteins / Q000032:analysis*; D011750:Pyroglobulins / Q000032:analysis*
D000606:Amino Sugars; D006023:Glycoproteins; D007075:Immunoglobulin M; D010266:Paraproteins; D011750:Pyroglobulins
Etude des monosaccharides aminés par détection thermoionique en chromatographie gaz-liquide. Application à une pyroglobuline IgM.