A real-time delay monitor for flow-system cell sorters.
For optimum performance in cell sorting, it is critical to assure proper timing in the charging of droplets to be deflected. A method for determining the transiet delay time in cell sorters has been devised and applied to daily operation in the Los Alamos sorter systems. This delay monitor relies on detection of either scattered or absorbed light from cells in the fluid stream near the point of droplet breakoff.
D002469:Cell Separation; D003584:Cytological Techniques / Q000295:instrumentation; D007834:Lasers; D008027:Light; D012542:Scattering, Radiation