Fatty acid and glycerol labeling of glycerolipids of leukocytes in response to ionophore A23187.
The effect of divalent cation ionophore, A23187, on the incorporation of [1-14C]palmitic acid, [1-14C]linoleic acid and [U-14C]glycerol into glycerolipids of polymorphonulcear leukocytes was examined. Ionophore A23187 stimulated the labeling of phosphatidic acid, phosphatidylglycerol, phosphatidylinositol, and diacylglycerol by both labeled fatty acids and glycerol. [1-14C]Palmitic acid and [1-14C]linoleic acid incorporation into phosphatidylcholine and triacylglycerol was reduced by the presence of the ionophore in the incubation medium, while [U-14C]glycerol labeling of these lipids was not significantly changed under identical conditions. These data reflect that the acylation of sn-glycerol 3-phosphate is activated, and the acylations of lysophosphatidyl-choline and endogenous diacylglycerol are inhibited in cells incubated with ionophore A23187. External calcium was not required for the ionophore effect on the incorporation of labeled fatty acids and glycerol. It is suggested that the ionophore alters the metabolism of the fatty acid and glycerol moieties of glycerolipids by changing the distribution of intracellular calcium of leukocytes.
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000001:Calcimycin / Q000494:pharmacology*; D005989:Glycerides / Q000097:blood*; D005990:Glycerol / Q000097:blood*; D006168:Guinea Pigs; D007700:Kinetics; D008041:Linoleic Acids / Q000097:blood*; D009504:Neutrophils / Q000187:drug effects / Q000378:metabolism*; D010169:Palmitic Acids / Q000097:blood*; D010743:Phospholipids / Q000097:blood*
D000900:Anti-Bacterial Agents; D005989:Glycerides; D008041:Linoleic Acids; D010169:Palmitic Acids; D010743:Phospholipids; D000001:Calcimycin; D005990:Glycerol