Demonstration of alpha 1-antitrypsin by immunofluorescence on paraffin-embedded hepatic and pancreatic tissue.
Studies were performed in an attempt to improve current immunohistological techniques for the demonstration of alpha 1-antitrypsin (A1AT) in formalin-fixed paraffin-embedded tissues. The unwanted fluorescence (UF) commonly occurring in such procedures was found to be effectively eliminated by immunoadsorption of A1AT antisera with human serum lacking A1AT (Pi-null phenotype) coupled in solid phase to glutaraldehyde-activated aminohexyl-Sepharose 4B. Specificity of the antisera for A1AT was established by subsequent solid phase immunoadsorption against normal human serum bound to AH-Sepharose 4B. Using these techniques, immunoreactive A1AT was demonstrated in the cytoplasm of hepatocytes in liver biopsies obtained from patients with Z and MZ serum phenotypes, and in the cytoplasm of normal pancreatic islet cells.
D000818:Animals; D005455:Fluorescent Antibody Technique; D006041:Goats; D006801:Humans; D007106:Immune Sera / Q000032:analysis; D008099:Liver / Q000032:analysis*; D010179:Pancreas / Q000032:analysis*; D010232:Paraffin; D010641:Phenotype; D011817:Rabbits; D014021:Tissue Preservation / Q000379:methods; D000515:alpha 1-Antitrypsin / Q000032:analysis* / Q000235:genetics
D007106:Immune Sera; D000515:alpha 1-Antitrypsin; D010232:Paraffin