Enzyme immunoassay of testosterone in plasma, with use of polyethylene glycol to separate antibody-bound and free hormone.
We describe an enzyme immunoassay for testosterone in which we use a testosterone-3-(carboxymethyl)oxime horseradish peroxidase conjugate as the label and an antiserum, raised in rabbits, to testosterone-3-(carboxymethyl)oxime-bovine serum albumin conjugate. Polyethylene glycol (Carbowax 6000) is used to separate antibody-bound and free steroid. The assay has a sensitivity of 12 pg/assay tube and satisfies the usual criteria of specificity, precision and accuracy. The results agree well with those obtained with a comparable radioimmunoassay.
D000818:Animals; D000936:Antigen-Antibody Complex; D003429:Cross Reactions; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008297:Male; D011092:Polyethylene Glycols; D011817:Rabbits; D011863:Radioimmunoassay; D013739:Testosterone / Q000097:blood*
D000936:Antigen-Antibody Complex; D011092:Polyethylene Glycols; D013739:Testosterone