title: Immunocytochemical localization of hyperglycemic hormone (HGH) in the neurosecretory system of the eyestalk of the crayfish Astacus leptodactylus.
abstract: Hyperglycemic hormone (HGH) from Astacus leptodactylus can be visualized by an immunocytochemical procedure using a specific antiserum against HGH and the peroxidase-anti-peroxidase (PAP) method. HGH containing cells are localized in the most distal portion of the x-organ in the medulla terminalis. Their cell diameter is about 47 +/- 7 micrometer; the nucleus measures about 22 +/- 2 micrometer. They form a subgroup of neurosecretory cell type 1, already described for the crayfish. A large amount of this HGH material is homogeneously stored in the sinus gland. A group of fibres in the x-organ - sinus gland - tractus can be followed, due to their positive reaction with the PAP-reagent.
mesh_terms: D000818:Animals; D003400:Astacoidea; D005123:Eye / Q000032:analysis*; D006651:Histocytochemistry; D006728:Hormones / Q000032:analysis*; D007124:Immunoenzyme Techniques; D009490:Neurosecretory Systems / Q000032:analysis*
chemical_list: D006728:Hormones