Improved method for the cyanogen bromide activation of agarose beads.
The significant new feature of the procedure is the reaction control of the BrCN activation merely by the slow transit of BrCN from a dispersed organic phase to the aqueous phase containing agarose beads in concentrated buffer. The product thus obtained was applied in a model immunoaffinity chromatographic separation. Experimental conditions are given for the control of the degree of activation and of the multiplicity of attachment of the protein ligand, for optimizing the immunological reactivity of the immunosorbent and for minimizing leakage of covalently bound protein from the resin.
D000818:Animals; D000941:Antigens / Q000032:analysis; D003488:Cyanogen Bromide; D007074:Immunoglobulin G; D007163:Immunosorbent Techniques; D011134:Polysaccharides; D011485:Protein Binding; D011817:Rabbits; D012685:Sepharose; D013552:Swine
D000941:Antigens; D007074:Immunoglobulin G; D011134:Polysaccharides; D012685:Sepharose; D003488:Cyanogen Bromide