The production and analysis of antilymphocyte sera following pregnancy and skin grafting of cattle.
This paper presents evidence to show that high titre antilymphocyte sera can be prepared more easily by skin grafting than from parous sera. It also shows that it is possible to analyse complex antilymphocyte sera by absorption with lymphocytes to produce working reagents. Following the 1 International BoLA Workshop the genotype of some of the animals used for skin grafting was ascertained and it was found that in each case where the resulting serum had been studied the main antiserum specificity that had been produced reacted with one of the BoLA specificities present in the skin donor.
D000818:Animals; D000918:Antibody Specificity; D000961:Antilymphocyte Serum; D002417:Cattle / Q000276:immunology*; D005260:Female; D005838:Genotype; D006649:Histocompatibility Antigens / Q000235:genetics / Q000276:immunology*; D007518:Isoantibodies; D008214:Lymphocytes / Q000276:immunology*; D011247:Pregnancy; D011270:Pregnancy, Animal; D016038:Skin Transplantation
D000961:Antilymphocyte Serum; D006649:Histocompatibility Antigens; D007518:Isoantibodies