Markers of human T cell subsets identified by alloantisera.
Analysis by the indirect fluorescence test followed by fluorescence-activated cell sorter (FACS) analysis has shown that antisera recognizing subsets of human T lymphocytes can be produced by planned immunizations involving HLA-A and HLA-B compatible donors. The reactivity of these antisera against some individuals of a population but not others shows that they recognize a polymorphic cell surface component. The reactive subpopulation largely overlaps with the JRA+ subset, which was previously shown to possess regulatory properties in functional assays. The specificity of the antisera for a T cell subset and the unrelatedness of the anti-B cell activity of the same antisera has been confirmed by two-color fluorescence tests.
D000918:Antibody Specificity; D001402:B-Lymphocytes / Q000276:immunology; D002965:Classification; D005455:Fluorescent Antibody Technique; D006680:HLA Antigens / Q000276:immunology; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology*; D007518:Isoantibodies; D010641:Phenotype; D013601:T-Lymphocytes / Q000276:immunology*
D006680:HLA Antigens; D007106:Immune Sera; D007518:Isoantibodies