In vitro propagation of Plasmodium falciparum for merozoite antigens.
A system with continuous flow of medium that uses disposable tissue culture flasks for the in vitro cultivation of P. falciparum is described. This scaled-up modification of the Trager and Jensen method gives merozoite yields sufficient for experimental vaccine studies in animals. A method of increasing the yield of merozoites by decreasing the pH of the medium is presented.
D000818:Animals; D000941:Antigens; D006801:Humans; D007158:Immunologic Techniques; D010963:Plasmodium falciparum / Q000254:growth & development / Q000276:immunology*
D000941:Antigens