The origin of alpha-1-acid glycoprotein (AGP) in human gastric juice.
In order to answer the question whether acid alpha 1-glycoprotein (AGP) is produced by the healthy human gastric mucosa, column chromatography of neutralized, pooled, and papain digested normal human gastric juice was performed on DEAE-cellulose using Varigrad NaCl elution gradients. Four glycoprotein fractions were obtained, one of which (fraction III) contained AGP. Its antibody raised in rabbits did not give immunofluorescence with any of the cells of the human gastric mucosa, only a reaction with AGP located in the intercellular spaces of gastric glands. These results indicate the extragastric origin of AGP, probably from serum.
D000818:Animals; D005455:Fluorescent Antibody Technique; D005750:Gastric Juice / Q000032:analysis*; D005753:Gastric Mucosa / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D009961:Orosomucoid / Q000032:analysis / Q000378:metabolism*; D011817:Rabbits
D009961:Orosomucoid