Comparison on the structure and transcriptional capability of growing phase and stationary yeast chromatin: a model for reversible gene activation.
We have compared the structure of intra-nuclear and isolated chromatin from logarithmically growing yeast cells to chromatin from cells which had entered the stationary phase and ceased growing. Both chromatins show a similar nucleosomal repeat pattern, 160 bp repeat size, with staphylococcal nuclease and similar variability in repeat sizes within the genome. DNase I produces the same ladder (less than 120 b) and a quite similar extended ladder (120-300 b) which shows that both chromatins have phased nucleosomes. However, the rate of DNase I digestion of growing phase is greater than in stationary. Functionally speaking, growing phase nuclei are 5-20 times as active in the rate of endogenous transcription (all three polymerases are involved). The transcriptional and DNase I susceptibility differences noted in nuclei are maintained in sucrose gradient isolated oligonucleosomes and mononucleosomes from the two states.
D000546:Amanitins / Q000494:pharmacology; D002467:Cell Nucleus / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism / Q000648:ultrastructure; D004247:DNA / Q000378:metabolism*; D003851:Deoxyribonucleases; D007700:Kinetics; D008836:Micrococcal Nuclease; D011188:Potassium / Q000494:pharmacology; D012313:RNA / Q000096:biosynthesis; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000378:metabolism*; D014158:Transcription, Genetic / Q000187:drug effects
D000546:Amanitins; D002843:Chromatin; D012313:RNA; D004247:DNA; D003851:Deoxyribonucleases; D008836:Micrococcal Nuclease; D011188:Potassium