Determination of 3,4-dihydroxyphenylethyleneglycol in urine by gas chromatography with a flame ionization detector: a new rapid method.
3,4-Dihydroxyphenylethyleneglycol, a major metabolite of noradrenaline in rat brain, is estimated alone or with 4-hydroxy-3-methoxyphenylethyleneglycol in rat and human urine by gas chromatography with a flame ionization detector. The samples are hydrolyzed and extracted at pH 2 with ethyl acetate. Then, to analyze only 3,4-dihydroxyphenylethyleneglycol the reaction with n-butaneboronic acid is carried out directly; if 4-hydroxy-3-methoxyphenylethyleneglycol also has to be estimated, preliminary acetylation in alkaline aqueous solution is performed. The advantages of the specificity due to the reagents used is discussed.
D015098:3-Methoxy-4-hydroxyphenylethanol / Q000652:urine; D000818:Animals; D002849:Chromatography, Gas / Q000379:methods; D006018:Glycols / Q000652:urine*; D006801:Humans; D007202:Indicators and Reagents; D008734:Methoxyhydroxyphenylglycol / Q000031:analogs & derivatives / Q000652:urine*; D051381:Rats
D006018:Glycols; D007202:Indicators and Reagents; D015098:3-Methoxy-4-hydroxyphenylethanol; D008734:Methoxyhydroxyphenylglycol