A secondary attachment site for bacteriophage lambda in trpC of E. coli.
We have determined the nucleotide sequence of a secondary lambda attachment site in trpC. Direct sequence analysis of lambdatrp transducing phage DNA fragments carrying the two prophage attachment sites reveals a 6 nucleotide homology in the crossover region which is a subset of the 15 nucleotide core sequence in the primary lambda attachment site: GCTTTTTTATACTAA. This 6 nucleotide sequence is also present in the intact trpC genome at the attachment site, as shown by analysis of trpC mRNA spanning this region.
D001483:Base Sequence; D002262:Carboxy-Lyases / Q000235:genetics*; D003090:Coliphages / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005796:Genes; D007209:Indole-3-Glycerol-Phosphate Synthase / Q000235:genetics*; D008242:Lysogeny; D009876:Operon; D012333:RNA, Messenger / Q000235:genetics; D011995:Recombination, Genetic
D012333:RNA, Messenger; D002262:Carboxy-Lyases; D007209:Indole-3-Glycerol-Phosphate Synthase