A simple method for the separation of tissue-cultured plant cells from unbound bacteria: a demonstration of acquired affinity for bacteria by cells of soy bean.
Tissue-cultured cells of soy bean, grown in liquid medium and then transferred to an agar substratum in so-called tissue chambers, showed an affinity for the binding of applied bacteria (Klebsiella pneumoniae). This binding, which became greater as the plant cells remained on the agar for up to 10 days, was manifested as an increasing resistance of the bacteria to be washed from the plant cells in a standard, gentle and reproducible procedure devised for this purpose. The number of bacteria firmly bound to the cells was determined by the use of 32P-labelled bacteria and was largely independent of the concentration of bacteria in the suspension applied to the tissue-cultured cells.
D000327:Adsorption; D001431:Bacteriological Techniques / Q000295:instrumentation; D002448:Cell Adhesion; D046508:Culture Techniques; D007711:Klebsiella pneumoniae / Q000302:isolation & purification*; D013025:Glycine max