A general method for maximizing the expression of a cloned gene.
We present a method, utilizing a combination of restriction endonuclease cleavage and digestion with Escherichia coli exonuclease III and Aspergillus orizae nuclease S1, that allows us to position a restriction fragment bearing the promoter of the lacZ gene of E. coli at virtually any distance in front of any cloned gene. In particular, we have used this method to examine the effect on protein production of gene-promoter separation for the cro gene of phage lambda and to produce plasmids that, upon transformation into appropriate E. coli hosts, direct the synthesis of up to 190,000 cro protein monomers per cell.
D001483:Base Sequence; D003090:Coliphages / Q000235:genetics; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D004274:DNA, Recombinant; D004720:Endonucleases / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D005809:Genes, Regulator; D007763:Lac Operon; D010957:Plasmids
D004269:DNA, Bacterial; D004274:DNA, Recombinant; D004720:Endonucleases; D004262:DNA Restriction Enzymes