An in vivo and in vitro study of an aging-related neuron cytoplasmic-binding antibody in male Fischer rats.
Fischer 344 male rats showed a low average level (less than ++) of in vivo as well as in vitro neuron binding antibody at 3, 15, and 26 months. However, a significant increase with age in average intensity of binding was observed with both the in vivo and the in vitro conditions. If a criterion of ++ is used as an antibody binding level likely to have biological consequences, the percent of in vivo cases showing foci of ++ or greater intensity also rises with age from 20 to 80%; a criterion of +++ gives an in vivo rise with age of only from 10 to 20%. The in vitro incidence with the ++ criterion is already 80% at three months and rises to 90%; with the +++ criterion the incidence with age rises from 20 to 80%. No evidence was obtained to support the concept that there is a weakening of the blood-brain-barrier in 26 month rats either by the systemic injection of trypan blue or by a comparison of intensity of antibody binding between extra- and intra-barrier neurones.
D000375:Aging; D000818:Animals; D001323:Autoantibodies / Q000032:analysis*; D001666:Binding Sites, Antibody; D001798:Blood Proteins / Q000032:analysis; D001812:Blood-Brain Barrier; D001921:Brain / Q000276:immunology; D003593:Cytoplasm / Q000276:immunology*; D007031:Hypothalamus / Q000276:immunology; D007124:Immunoenzyme Techniques; D066298:In Vitro Techniques; D008297:Male; D009474:Neurons / Q000166:cytology / Q000276:immunology*; D051381:Rats
D001323:Autoantibodies; D001798:Blood Proteins