Measurement of macromolecular equilibrium binding constants by a sucrose gradient band sedimentation method. Application to protein-nucleic acid interactions.
D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism*; D001665:Binding Sites; D002417:Cattle; D002499:Centrifugation, Density Gradient / Q000379:methods; D003090:Coliphages; D004247:DNA / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism; D004926:Escherichia coli; D046911:Macromolecular Substances; D010179:Pancreas / Q000201:enzymology; D011485:Protein Binding; D012260:Ribonucleases / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000378:metabolism*
D001426:Bacterial Proteins; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D046911:Macromolecular Substances; D012269:Ribosomal Proteins; D004247:DNA; D012260:Ribonucleases