Complement-mediated unspecific binding of immunoglobulins to some endocrine cells.
Unspecific binding of immunoglobulins to gastrin G cells, glucagon A cells and somatostatin D cells of the gastric mucosa or pancreas, as well as to the calcitonin-somatostatin cells of rabbit thyroid has been found to occur through a non antigen-antibody mechanism mediated at least in part by the C1q fraction of complement. The phenomenon represents a major drawback in hormone immunohistochemistry, which can be prevented by incubating the specific anti-hormone sera with anti-C1q antibodies or with complement-fixing immunocomplexes.
D000818:Animals; D003165:Complement System Proteins / Q000378:metabolism*; D006224:Cricetinae; D005753:Gastric Mucosa / Q000378:metabolism*; D006168:Guinea Pigs; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism*; D007158:Immunologic Techniques; D007515:Islets of Langerhans / Q000378:metabolism*; D011485:Protein Binding; D011817:Rabbits; D013552:Swine
D007136:Immunoglobulins; D003165:Complement System Proteins