A technical improvement for crossed immunoelectrophoresis.
A simple antibody overlay technique for crossed immunoelectrophoresis is described. After the initial electrophoresis of antigens, the gel is overlaid with antibody-impregnated filter paper. This makes it necessary to pour only one gel, simplifying technique as compared with the conventional method.
D000818:Animals; D000942:Antigens, Bacterial; D007122:Immunoelectrophoresis / Q000379:methods*; D007123:Immunoelectrophoresis, Two-Dimensional / Q000379:methods*; D011817:Rabbits; D012486:Salmonella typhimurium / Q000276:immunology; D013997:Time Factors
D000942:Antigens, Bacterial