Direction of deoxyribonucleic acid transfer and replication in a derivative of plasmid R100-1.
The site of integration and the molecular orientation of a prophage Mu integrated within the resistance transfer factor component of plasmid R100-1 have been determined on the physical map of the plasmid. This allowed us (i) to determine the direction of deoxyribonucleic acid transfer from oriT during conjugation and (ii) to demonstrate the unidirectionality of replication in conditions of exponential growth (by determining the strand preference of Mu-specific Okazaki fragments).
D002874:Chromosome Mapping; D003090:Coliphages / Q000235:genetics; D003227:Conjugation, Genetic; D004261:DNA Replication; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics*; D004270:DNA, Circular / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D011815:R Factors
D004269:DNA, Bacterial; D004270:DNA, Circular; D004262:DNA Restriction Enzymes