Escherichia coli phosphoenolpyruvate dependent phosphotransferase system. Copurification of HPr and alpha 1-6 glucan.
A rapid, high-yield procedure has been developed for the purification of HPr from the Escherichia coli phosphoenolpyruvate dependent phosphotransferase system. During this procedure, the protein copurifies with a 2500-dalton homopolysaccharide which we have identified as alpha 1-6 glucan. The results of steady-state kinetic measurements of the phosphotransferase activity demonstrate that the polysaccharide works as an activator of the phosphotransferase system probably at the level of the HPr:P-E1 complex or the P-HPr:E11 complex.
D001426:Bacterial Proteins / Q000302:isolation & purification*; D002241:Carbohydrates / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D004926:Escherichia coli / Q000201:enzymology*; D005936:Glucans / Q000302:isolation & purification*; D009682:Magnetic Resonance Spectroscopy; D008954:Models, Biological; D008968:Molecular Conformation; D008970:Molecular Weight; D010710:Phosphates / Q000032:analysis; D010728:Phosphoenolpyruvate / Q000378:metabolism*; D010770:Phosphotransferases / Q000378:metabolism*; D011135:Polysaccharides, Bacterial / Q000032:analysis*
D001426:Bacterial Proteins; D002241:Carbohydrates; D005936:Glucans; D010710:Phosphates; D011135:Polysaccharides, Bacterial; D010728:Phosphoenolpyruvate; D010770:Phosphotransferases