[Use of a pyrimidine-dependent mutant for effective labeling of the DNA OF Aspergillus nidulans].
Incorporation of the radioactive label from pyrimidines into RNA and DNA of an Aspergillus nidulans pyrimidine-dependent mutant was studied. The label from [14C2]uridine was incorporated at the highest rate into both fractions of nucleic acids from A. nidulans of the wild type and pyr-mutant. The content of the label in the DNA of the pyr-mutant was several times higher than that in the wild type DNA. Therefore, pyr-mutants can be used in order to study molecular-genetic processes in the cells of A. nidulans. The label from [3H5]deoxyuridine, [14C2]thymine, [14C2]thymidine, [14C2]cytosine and I114C2]uracil was incorporated into the pyr-mutant RNA and DNA at a much lower rate than into the wild type RNA and DNA. Apparently, these pyrimidines cannot maintain the growth of the pyr-mutant. Incorporation of the label from [14C2]uracil into the pyr-mutant DNA increased if growth of the mycelium was stimulated by low concentrations of unlabeled uridine.
D001233:Aspergillus nidulans / Q000235:genetics / Q000378:metabolism*; D003596:Cytosine / Q000378:metabolism; D004247:DNA / Q000378:metabolism*; D003857:Deoxyuridine / Q000378:metabolism; D007553:Isotope Labeling; D009154:Mutation; D011743:Pyrimidines / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D013936:Thymidine / Q000378:metabolism; D013941:Thymine / Q000378:metabolism; D014498:Uracil / Q000378:metabolism; D014529:Uridine / Q000378:metabolism
D011743:Pyrimidines; D014498:Uracil; D012313:RNA; D003596:Cytosine; D004247:DNA; D013941:Thymine; D013936:Thymidine; D003857:Deoxyuridine; D014529:Uridine
Ispol'zovanie pirimidinzavisimogo mutanta dlia éffektivnogo mecheniia DNK Aspergillus nidulans.