In vivo studies on the incorporation of microinjected acidic proteins of the large ribosomal subunit from Escherichia coli and artermia salina into oocyte ribosomes from Xenopus laevis.
Tritium-labeled acidic proteins from the large ribosomal subunit of Artermia salina or Escherichia coli were microinjected into the cytoplasm of stage IV/V oocytes from Xenopus laevis. eL12 from the large ribosomal subunit of A. salina but not L7/L12 or L7/L12--L10 from E. coli is specifically incorporated into 60S ribosomal subunits of oocytes. This incorporation is not significantly inhibited by actinomycin D. Incorporation of eL12 into the 60S subunits occurs in enucleated oocytes, suggesting that active ribosomal ribonucleic acid synthesis and ribosome assembly as well are not prerequired for this reaction. Apparently the incorporation proceeds via an exchange reaction between a free cytoplasmic pool of eL12 and ribosomal eL12.
D000818:Animals; D001156:Artemia; D001426:Bacterial Proteins / Q000378:metabolism*; D002458:Cell Fractionation; D003609:Dactinomycin / Q000494:pharmacology; D004926:Escherichia coli / Q000032:analysis; D005260:Female; D008845:Microinjections; D009865:Oocytes / Q000378:metabolism*; D010063:Ovum / Q000378:metabolism*; D012335:RNA, Ribosomal / Q000096:biosynthesis; D012269:Ribosomal Proteins / Q000378:metabolism*; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D013347:Subcellular Fractions / Q000378:metabolism; D014529:Uridine / Q000378:metabolism; D014981:Xenopus
D001426:Bacterial Proteins; D012335:RNA, Ribosomal; D012269:Ribosomal Proteins; D003609:Dactinomycin; D014529:Uridine