Synthesis of the nascent strand of tRNAfMet from E coli.
Oligonucleotides corresponding to the total tRNAfMET FROM E. coli have been synthesized. These fragments were joined by using RNA ligase to yield quarter molecules. The 5'-quarter molecule showed 84% amino acid acceptor activity when it was combined with the natural three quarter molecule.
D001483:Base Sequence; D004926:Escherichia coli / Q000032:analysis*; D008722:Methods; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000138:chemical synthesis*; D009843:Oligoribonucleotides / Q000138:chemical synthesis*; D011130:RNA Ligase (ATP); D012343:RNA, Transfer / Q000138:chemical synthesis*; D012358:RNA, Transfer, Met
D009841:Oligonucleotides; D009843:Oligoribonucleotides; D012358:RNA, Transfer, Met; C024988:tRNA, formylmethionine-; D012343:RNA, Transfer; D011130:RNA Ligase (ATP)