Electrolyte effects on the activity of mutant enzymes in vivo and in vitro.
All temperature-sensitive histidine auxotrophs of Salmonella typhimurium tested are corrected by addition of neutral salts to their growth medium. The correctability seems to result from direct electrolyte effects on mutant protein stability since several of the mutant proteins are also salt correctable in vitro.
D000429:Alcohol Oxidoreductases / Q000378:metabolism*; D000645:Ammonium Sulfate / Q000494:pharmacology; D003433:Crosses, Genetic; D006639:Histidine / Q000378:metabolism*; D006641:Histidinol; D007700:Kinetics; D009154:Mutation; D012483:Salmonella Phages; D012486:Salmonella typhimurium / Q000201:enzymology*; D012965:Sodium Chloride / Q000494:pharmacology; D013045:Species Specificity; D014161:Transduction, Genetic
D012965:Sodium Chloride; D006639:Histidine; D006641:Histidinol; D000429:Alcohol Oxidoreductases; D000645:Ammonium Sulfate