Carbohydrate content of acid alpha-glucosidase (gamma-amylase) from human liver.
The presence of carbohydrates in homogeneous preparations of human liver acid alpha-glucosidase has been established and the carbohydrate content of the enzyme determined. The enzyme was purified with the specific purpose of removing all low-molecular-weight carbohydrates. It was specifically adsorbed on Concanavalin A-Sepharose, eluted with methyl-alpha-D-mannopyranoside and gave a positive reaction with the phenol-sulphuric acid reagent. These facts taken together provide evidence that the enzyme studied is a glycoprotein. The analysis of the carbohydrate content of human liver acid alpha-glucosidase showed that there were 8.3 glucosamine, 13.2 mannose and possibly 3--4 glucose residues per molecule of the enzyme with a molecular weight of 98,000.
D005944:Glucosamine / Q000032:analysis; D005947:Glucose / Q000032:analysis; D005959:Glucosidases / Q000032:analysis*; D006801:Humans; D008099:Liver / Q000201:enzymology*; D008358:Mannose / Q000032:analysis; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis*; D000520:alpha-Glucosidases / Q000032:analysis* / Q000302:isolation & purification
D009005:Monosaccharides; D005959:Glucosidases; D000520:alpha-Glucosidases; D005947:Glucose; D005944:Glucosamine; D008358:Mannose