[Isolation of free cytoplasmic informosomes].
The review concerns methods used by different authors for isolation of free cytoplasmic informosomes. The results of centrifugation in sucrose gradient and in density gradients, electrophoresis, ion-exchange, gel-filtration and affinity methods are described. It is concluded that these methods do not give good purification of informosomes from ribosomes and free proteins. Urgent necessity of a more careful control of the purity of informosomes isolated is emphasized.
D002499:Centrifugation, Density Gradient; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003593:Cytoplasm / Q000032:analysis; D004247:DNA / Q000302:isolation & purification*; D004586:Electrophoresis; D008970:Molecular Weight; D009698:Nucleoproteins / Q000302:isolation & purification; D012313:RNA / Q000302:isolation & purification*; D012270:Ribosomes / Q000032:analysis
D009698:Nucleoproteins; D012313:RNA; D004247:DNA
Problema preparativnogo vydeleniia svobodnykh tsitoplazmaticheskikh informosom.