Virus-like particles and double stranded RNA from killer and non-killer strains of Saccharomyces cerevisiae.
Virus-like particles and DsRNA found in extracts of killer, non-killer and suppressive non-killer strains were co-precipitated from cell extracts using an antibody prepared against purified virus-like particles isolated from a non-killer strain having only the higher molecular weight L dsRNA. The relative amount of virus-like particles correlated roughly with the amount of dsRNA: those strains with high concentrations of dsRNA had the most particles. When a preparation of particles was subjected to sucrose gradient velocity centrifugation, particles containing the S and M dsRNA could be separated from those containing the L dsRNA. These experiments taken together suggest that the L, M and S dsRNAs are separately encapsulated by the same protein coat.
D002213:Capsid / Q000032:analysis; D011233:Precipitin Tests; D012313:RNA / Q000032:analysis*; D012441:Saccharomyces cerevisiae / Q000032:analysis* / Q000502:physiology; D014771:Virion / Q000302:isolation & purification*
D012313:RNA