A method for the ultrastructural examination of cell monolayers cultured in plastic microtitre plates.
A method is presented by which cells growing as monolayers cultured in microtitre plates can be embedded for electron microscopy. The technique has the following advantages: numerous specimens may be prepared with relatively small numbers of cells, cell-cell interactions remain undisturbed and may be enumerated, and morphology can be studied under circumstances identical with those used in cell-mediated cytotoxicity assays.
D000818:Animals; D002478:Cells, Cultured / Q000648:ultrastructure*; D003584:Cytological Techniques; D003602:Cytotoxicity, Immunologic; D008214:Lymphocytes / Q000648:ultrastructure; D008223:Lymphoma / Q000648:ultrastructure; D051379:Mice; D008854:Microscopy, Electron; D009374:Neoplasms, Experimental / Q000648:ultrastructure