Solid-phase enzyme immunoassay for serum ferritin.
We describe a solid-phase enzyme immunoassay for ferritin in human serum, with use of a horseradish peroxidase-labeled antibody and a highly sensitive chromogen, 2,2'-azino-di(3-ethyl-benzthiazoline-6-sulfonate). The assay requires only 10 microL of serum per assay, relatively less time and labor than other assys for ferritin, and as little as 10 pg of ferritin can be measured. We believe this assay offers a reliable alternative to radioimmunoassay for serum ferritin.
D005293:Ferritins / Q000097:blood*; D006735:Horseradish Peroxidase; D006801:Humans; D007124:Immunoenzyme Techniques; D007202:Indicators and Reagents; D007700:Kinetics
D007202:Indicators and Reagents; D005293:Ferritins; D006735:Horseradish Peroxidase