Malignant behaviour of three adenovirus-2-transformed brain cell lines and their methyl cellulose-selected sub-clones.
Three adenovirus-2-transformed rat embryo brain cell lines and their methylcellulose-selected sub-clones were examined for fibronectin expression, anchorage-independent growth, saturation density, T antigen expression and morphology. Tumorigenicity studies were carried out on newborn and ATS immunosuppressed syngeneic rats and congenitally athymic nude mice. With one exception the methylcellulose sub-clones contained significantly fewer fibronectin-positive cells than the parent lines; a number of sub-clones contained no fibronectin-positive cells. Methylcellulose selection did not always alter cell morphology, saturation density or anchorage-independent growth as compared with parent lines. However, the methylcellulose sub-clones were considerably more malignant than the parent cell lines as measured by invasion and metastasis in nude mice. No in vitro characteristic correlated with malignant behaviour.
D000256:Adenoviridae / Q000276:immunology; D000818:Animals; D000956:Antigens, Viral; D000961:Antilymphocyte Serum / Q000494:pharmacology; D001921:Brain; D002460:Cell Line; D002469:Cell Separation / Q000379:methods; D002471:Cell Transformation, Neoplastic; D002472:Cell Transformation, Viral; D002999:Clone Cells; D003470:Culture Media; D005260:Female; D005353:Fibronectins / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D008297:Male; D008747:Methylcellulose; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000209:etiology* / Q000473:pathology; D051381:Rats; D013601:T-Lymphocytes / Q000276:immunology; D014185:Transplantation, Isogeneic
D000956:Antigens, Viral; D000961:Antilymphocyte Serum; D003470:Culture Media; D005353:Fibronectins; D008747:Methylcellulose