Detection of measles virus antigen(s) in peripheral lymphocytes from patients with subacute sclerosing panencephalitis.
Peripheral blood lymphocytes from 5 patients with subacute sclerosing panencephalitis (SSPE) were stimulated with phytohemagglutinin (PHA) and analyzed for the presence of the measles virus antigen(s) by immunofluorescence (IF). For detection of viral antigen fluorescein-conjugated globulins from SSPE patients or from measles convalescents both with high anti-measles titer were used. Peripheral blood lymphocytes from 5 children with measles were used as a positive control. Measles virus antigen(s) were localized in PHA-transformed lymphocytes in all SSPE patients. The present results indicate that in SSPE measles-like virus infection may be found not only in the central nervous system, but also in the circulating lymphoid cells.
D000293:Adolescent; D000956:Antigens, Viral / Q000032:analysis*; D002648:Child; D002675:Child, Preschool; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology*; D008297:Male; D008457:Measles / Q000276:immunology; D008459:Measles virus / Q000276:immunology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D013344:Subacute Sclerosing Panencephalitis / Q000276:immunology*
D000956:Antigens, Viral; D010835:Phytohemagglutinins