Specific inhibition of macrophage migration inhibition factor by fucosylated glycolipid RM.
The effects of glycolipids on the interaction of the MIF (migration inhibition factor) with rat macrophages were examined using a migration inhibition assay system. MIF activity was specifically blocked by fucosylated Glycolipid RM [Gal alpha 1-3Gal(2-1 alpha Fuc) beta 1-3GalNAc beta 1-3Gal beta 1-4Glc beta 1-1ceramide, (1978) J. Biochem. 83, 85-90], but not by Cytolipin R, hematoside, or blood group B active glycolipid [Gal alpha 1-3Gal(2-1 alpha Fuc) beta 1-4GlcNAc beta 1-3Gal beta 1-4Glc beta 1-1ceramide]. Inhibition of MIF activity was proportional to the concentration of Glycolipid RM. These findings suggest that Glycolipid RM acts as a receptor for MIF.
D000818:Animals; D005643:Fucose / Q000494:pharmacology; D005679:G(M3) Ganglioside / Q000494:pharmacology; D005915:Globosides / Q000494:pharmacology*; D006017:Glycolipids / Q000494:pharmacology; D006028:Glycosphingolipids / Q000494:pharmacology*; D008263:Macrophage Migration-Inhibitory Factors / Q000037:antagonists & inhibitors*; D008297:Male; D051381:Rats
D005679:G(M3) Ganglioside; D005915:Globosides; D006017:Glycolipids; D006028:Glycosphingolipids; D008263:Macrophage Migration-Inhibitory Factors; D005643:Fucose