title: Total synthesis of a tyrosine suppressor tRNA gene. XV. Synthesis of the promoter region.
abstract: By use of polynucleotide kinase and polynucleotide ligase, the 10 deoxyoligonucleotide segments, whose syntheses have been described in accompanying papers, have been joined to form the 62-nucleotide-long DNA corresponding to the promoter region of an Escherichia coli suppressor tRNA gene. The following sequence in the joining reactions was used to obtain error-free and optimal yields of the products: 1) joining of Segment P-1 to P-3 in the presence of Segment P-2; 2) joining of Segments P-4 to P-7 to form Duplex [P4-7]; 3) joining of Segments P-8 to P-10 to Duplex [P4-7] to form Duplex [P4-10]; and finally, 4) joining of P-(1 + 3) and P-2 to Duplex [P4-10] to form the total promoter Duplex [P].
mesh_terms: D001483:Base Sequence; D004269:DNA, Bacterial / Q000138:chemical synthesis*; D004926:Escherichia coli; D007202:Indicators and Reagents; D008722:Methods; D012343:RNA, Transfer / Q000096:biosynthesis*; D013489:Suppression, Genetic; D014443:Tyrosine
chemical_list: D004269:DNA, Bacterial; D007202:Indicators and Reagents; D014443:Tyrosine; D012343:RNA, Transfer