A rapid, semi-automated procedure for the evaluation of leukocyte locomotion in the micropore filter assay.
A commerically available bacterial colony counter has been modified to allow rapid, accurate, semi-automated evaluation of cell numbers in the micropore filter assay for chemotaxis. The method is valuable for objective, rapid evaluation of cell counts at various levels through the filter, as well as counts on the distal surface of the filter. Coupled with a programmable calculator, this instrument had made feasible a new method of assessing random migration by the regression line analysis, which discriminates between migration rate and mass migration of cells. This combination of equipment may thus serve as a considerable time saving accessory to laboratories engaged in cell locomotion research, but also will allow more rigorous assessment of differences among specific populations of cells.
D000704:Analysis of Variance; D001322:Autoanalysis; D000001:Calcimycin / Q000494:pharmacology; D002452:Cell Count; D002464:Cell Migration Inhibition; D002465:Cell Movement; D002634:Chemotaxis, Leukocyte; D005260:Female; D005374:Filtration / Q000295:instrumentation; D006801:Humans; D007962:Leukocytes / Q000276:immunology*; D008297:Male; D013997:Time Factors
D000001:Calcimycin