[Transforming activity of R- and Lac-plasmids of clinical strains of Gram-negative bacteria].
The isolated plasmid DNA of clinical strains of Gram-negative bacteria were shown to have transforming activity when E. coli strain 0600 and S. typhimurium strain LT-2 were used as recipients. The frequency of transformation depended on the recipient strain and the character of the plasmids. The presence of deletion mutants was revealed among the transformants. Such mutants occurred with varying frequency, most often in S. typhimurium strain LT-20; the reason for this phenomenon is at present under discussion. The transformation of plasmids controlling lactose splitting and their conjugation transfer into recipient S. typhimurium strain LT-2 is possible only under condition of using recipient (R+). The possibility of the formation of the cointegrate (R and lac plasmids) in recipient S. typhimurium strain LT-2 is discussed.
D004269:DNA, Bacterial; D004926:Escherichia coli / Q000235:genetics*; D007786:Lactose Factors; D010957:Plasmids; D011815:R Factors; D012486:Salmonella typhimurium / Q000235:genetics*; D014169:Transformation, Bacterial
D004269:DNA, Bacterial
Transformiruiushchaia aktivnost' R- i Lac-plazmid klinicheskikh shtammov gramotritsatel 'nykh bakteriĭ.