A method for the assay of ablastin in the serum of rats infected with Trypanosoma lewisi.
This paper describes a simple quantitative assay for albastin, a factor present in the serum from rats infected with Trypanosoma lewisi, which prevents the division of the parasite. The assay measures in vitro the inhibition of the incorporation of 3H-TdR into the DNA of T. lewisi in the presence of serum from infected animals. Utilising this method, one can measure the titre of albastin in a particular serum sample and the time and duration of its appearance in the circulation of infected rats.
D000818:Animals; D002455:Cell Division; D004247:DNA / Q000096:biosynthesis; D007074:Immunoglobulin G; D007700:Kinetics; D051381:Rats; D014344:Trypanocidal Agents / Q000097:blood*; D014350:Trypanosoma lewisi / Q000254:growth & development* / Q000378:metabolism; D014352:Trypanosomiasis / Q000097:blood*
D007074:Immunoglobulin G; D014344:Trypanocidal Agents; C019230:ablastin; D004247:DNA