Isolation and characterisation of a yeast chromosomal replicator.
A yeast DNA sequence that behaves as a chromosomal replicator, ars1 (autonomously replicating sequence), has been isolated. On transformation, ars1 allows autonomous replication of all co-linear DNA. The replicator can integrate into other replication units and can function in multimeric form. The 850-base pair ars1 element has no detectable homology to other yeast sequences. Such replicator-containing plasmids can be used for the isolation of DNA sequences in yeast cells as well as for the study of chromosomal DNA replication.
D001483:Base Sequence; D002875:Chromosomes / Q000032:analysis; D004261:DNA Replication; D004271:DNA, Fungal / Q000235:genetics* / Q000302:isolation & purification; D004274:DNA, Recombinant; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014170:Transformation, Genetic
D004271:DNA, Fungal; D004274:DNA, Recombinant