Human interferon production with diploid fibroblast cells grown on microcarriers.
A variety of diploid human fibroblast lines have been successfully grown to high densities (greater than 10(6) cell/ml) on recently developed microcarriers. Interferon induction using poly I.poly C and a superinduction procedure resulted in yields greater than 10,000 units/ml with one cell line. A direct comparison of microcarrier cultures to roller bottle cultures showed equivalent interferon yields on a per cell basis and some apparent differences relating to optimum inducer concentrations and kinetics of interferon accumulation.
D002460:Cell Line; D004171:Diploidy; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D007372:Interferons / Q000032:analysis / Q000096:biosynthesis*; D007700:Kinetics; D008828:Microbiological Techniques; D011066:Poly C / Q000494:pharmacology; D011069:Poly I / Q000494:pharmacology
D011069:Poly I; D011066:Poly C; D007372:Interferons