Prophage substitution and prophage loss from superinfected Escherichia coli recA(P1) lysogens.
It is shown that the plasmid prophage P1 can be displaced by a superinfecting P1 phage in Escherichia coli recA(P1) lysogens. Six widely separated phage markers were used to distinguish between residual recombination and total substitution. It is further shown that superinfection of recA lysogens can lead to loss of both phage (curing). These two phenomena, previously reported in Rec+ strains, are thus independent of host recombination and may result from perturbations of some function involved in plasmid maintenance.
D002701:Chloramphenicol / Q000494:pharmacology; D003090:Coliphages / Q000235:genetics* / Q000254:growth & development; D004926:Escherichia coli / Q000235:genetics*; D007612:Kanamycin / Q000494:pharmacology; D008242:Lysogeny; D009154:Mutation; D011815:R Factors; D011995:Recombination, Genetic; D014161:Transduction, Genetic; D014779:Virus Replication
D007612:Kanamycin; D002701:Chloramphenicol