Biosynthesis of somatostatin in pancreatic islets of Wistar rats.
Pancreatic islets of Wistar rats were isolated by collagenase digestion and incubated with [3H]-L-phenylalanine. Using a specific somatostatin antiserum radioactivity was found in the antibody-antigen-complex. The radioactivity was displaced by unlabelled somatostatin. These findings give the first evidence for the biosynthesis of somatostatin or somatostatin-like peptides in mammalian pancreatic islets.
D000818:Animals; D005260:Female; D007515:Islets of Langerhans / Q000378:metabolism*; D051381:Rats; D013004:Somatostatin / Q000096:biosynthesis*
D013004:Somatostatin