Consecutive demonstration of catecholamines and dopamine-beta-hydroxylase within the same specimen.
A combined method was developed for characterization and differentiation of catecholamines in neuron populations containing more than one catecholamine, e.g. dopamine and norepinephrine. Its application to small intensely fluorescent (SIF-) cell clusters in sympathetic ganglia allows the successive demonstration of glyoxylic acid-induced catecholamine fluorescence and dopamine-beta-hydroxylase by indirect immunofluorescence within the same tissue section. Applying this technique to an example, two types of SIF-cells were demonstrated in the guinea pig superior cervical ganglion.
D000818:Animals; D002395:Catecholamines / Q000032:analysis*; D004299:Dopamine beta-Hydroxylase / Q000032:analysis*; D005260:Female; D005455:Fluorescent Antibody Technique; D005728:Ganglia, Sympathetic / Q000166:cytology* / Q000201:enzymology; D006168:Guinea Pigs; D008856:Microscopy, Fluorescence
D002395:Catecholamines; D004299:Dopamine beta-Hydroxylase