title: Transmembrane communication in cells chronically infected with measles virus.
abstract: The transmembrane association of the measles virus hemagglutinin and hemolysin surface proteins with intracellular viral antigens was studied. Rabbit antisera monospecific for measles virus matrix and nucleocapsid proteins and a human antiserum containing specificities for both the hemagglutinin and hemolysin proteins were used to study the co-capping of these proteins in human Lu 106 cell-line, chronically infected with measles virus. Capping of the surface-associated envelope components was accompanied by co-capping of the matrix and nucleocapsid proteins, the latter being localized mainly within the inclusions. This demonstrated transmembrane communication between surface-associated envelope components and the intracellular measles virus matrix and nucleocapsid proteins. The results demonstrated the existence of a linkage between viral inclusions and viral proteins associated with cell membranes. In the presence of cytochalasin B (1--2 micrograms/ml), co-capping of the matrix protein was unchanged or slightly enhanced, whereas co-capping of the nucleocapsid protein decreased, indicating that actin filaments may mediate the communication between viral nucleocapsids and the cell membrane.
mesh_terms: D000954:Antigens, Surface / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology; D002460:Cell Line; D002478:Cells, Cultured; D003571:Cytochalasin B / Q000494:pharmacology; D005455:Fluorescent Antibody Technique; D006389:Hemagglutinins, Viral / Q000276:immunology; D006460:Hemolysin Proteins / Q000276:immunology; D006801:Humans; D007152:Immunologic Capping / Q000187:drug effects; D008459:Measles virus / Q000276:immunology*; D012367:RNA, Viral / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology
chemical_list: D000954:Antigens, Surface; D000956:Antigens, Viral; D006389:Hemagglutinins, Viral; D006460:Hemolysin Proteins; D012367:RNA, Viral; D014764:Viral Proteins; D003571:Cytochalasin B