Electron microscopic definition of surface antigens of group B Streptococcus.
Strains representing each of the five serologically distinct types of group B Streptococcus and variant strain 090R, which is devoid of type-specific polysaccharide, were studied by electron microscopy after incubation with various ferritin-conjugated antibodies. Ferritin-conjugated types Ia, II and III antibodies localized on homologous cells in a pattern that defines the capsular location of the type Ia, II, and III polysaccharide antigen. Type Ib and Ic cells have surface antigens to which ferritin-labeled antibodies to both capsular polysaccharide and a common surface protein attach.
D000818:Animals; D000937:Antigen-Antibody Reactions; D000954:Antigens, Surface / Q000032:analysis*; D005293:Ferritins; D007158:Immunologic Techniques; D011135:Polysaccharides, Bacterial / Q000032:analysis / Q000276:immunology; D011817:Rabbits; D013292:Streptococcus agalactiae / Q000276:immunology* / Q000648:ultrastructure
D000954:Antigens, Surface; D011135:Polysaccharides, Bacterial; D005293:Ferritins