Synthesis of biologically active portions of an intercistronic region by use of a new 3'-phosphate incorporation method to protect 3'-OH and their binding to ribosomes.
D001426:Bacterial Proteins / Q000096:biosynthesis*; D001483:Base Sequence; D055598:Chemical Phenomena; D002621:Chemistry; D003062:Codon; D004926:Escherichia coli / Q000378:metabolism; D008722:Methods; D009841:Oligonucleotides / Q000138:chemical synthesis*; D009843:Oligoribonucleotides / Q000138:chemical synthesis*; D010442:Peptide Chain Initiation, Translational; D011116:Polynucleotide 5'-Hydroxyl-Kinase; D011130:RNA Ligase (ATP) / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism
D001426:Bacterial Proteins; D003062:Codon; D009841:Oligonucleotides; D009843:Oligoribonucleotides; D011116:Polynucleotide 5'-Hydroxyl-Kinase; D011130:RNA Ligase (ATP)