Synthesis of cytoplasmic membrane during growth and division of Escherichia coli. Dispersive behaviour of respiratory nitrate reductase.
We have used the penicillin selection method of Autissier & Kepes [(1972) Biochimie 54, 93--101] to study the segregation of membrane-bound respiratory nitrate reductase (EC 1.9.6.1) in Escherichia coli for the three generations after cessation of nitrate reductase synthesis caused by withdrawal of nitrate from the growth medium. We also included a physical separation procedure that permitted direct assay for nitrate reductase activity among all fractions produced by the penicillin selection method. We conclude that the segregation of nitrate reductase after cell division is dispersive, and not semi-conservative as proposed by Autissier & Kepes (1972).
D002455:Cell Division; D002499:Centrifugation, Density Gradient; D003593:Cytoplasm / Q000201:enzymology / Q000502:physiology; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development*; D007425:Intracellular Membranes / Q000201:enzymology / Q000502:physiology; D009565:Nitrate Reductases / Q000378:metabolism*; D010400:Penicillin G / Q000494:pharmacology
D009565:Nitrate Reductases; D010400:Penicillin G