title: Proteus mirabilis phage 5006M: a physical characterization.
abstract: This report deals with physical characterization of the generalized transducing Proteus mirabilis phage 5006M. The morphology of the phage is presented, the buoyant density was determined (1.491 g/ml) and the G + C content of the phage DNA was found to be 44%. The phage genome has a length of 14.8 micrometers and mol. wt. of 30.7 x 10(6). Denaturation mapping revealed non-random circular permutation of the phage DNA. The genome exhibits 3.6% terminal redundancy as shown by homoduplex analysis. The existence of concatemeric precursors of phage 5006M DNA is inferred and the results are interpreted in terms of a sequential headful packaging mechanism.
mesh_terms: D001435:Bacteriophages / Q000648:ultrastructure*; D003596:Cytosine / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis*; D006147:Guanine / Q000032:analysis; D008970:Molecular Weight; D009691:Nucleic Acid Denaturation; D009695:Nucleic Acid Renaturation; D011513:Proteus mirabilis
chemical_list: D004279:DNA, Viral; D006147:Guanine; D003596:Cytosine