Passive immune hemolysis for detection of heat-labile enterotoxin produced by Escherichia coli isolated from different sources.
Fifty-one strains of Escherichia coli isolated from humans, swine, food, and water and identified as enterotoxinogenic by the Y-1 adrenal cell assay, were examined for heat-labile enterotoxin (LT) production by the passive immune hemolysis test. Cholera antitoxin, anti-choleragenoid and anti-LT were used as antisera. Cholera antitoxin was much more potent than anti-choleragenoid and LT antiserum in the detection of LT-positive strains. All strains isolated from pigs and sausage were negative in tests made with LT antiserum. A few strains isolated from humans, food, and water also gave negative results. These data showed that the passive immune hemolysis test is not as efficient as the Y-1 adrenal cell assay in the detection of enterotoxinogenic E. coli strains.
D001427:Bacterial Toxins / Q000032:analysis / Q000096:biosynthesis*; D004768:Enterotoxins / Q000032:analysis / Q000096:biosynthesis*; D004926:Escherichia coli / Q000378:metabolism*; D006461:Hemolysis; D007158:Immunologic Techniques
D001427:Bacterial Toxins; D004768:Enterotoxins