Electron microscopical-immunocytochemical evidence of ecdysteroids in the prothoracic gland of Galleria mellonella.
Fixation of prothoracic glands of Galleria mellonella with a solution containing saponin permits immunocytochemical staining of the entire gland. By this means ecdysteroids were demonstrated electron microscopically to be present in the hyaloplasm and microtubules.
D000818:Animals; D002462:Cell Membrane / Q000032:analysis; D004440:Ecdysone / Q000032:analysis*; D004441:Ecdysterone / Q000032:analysis*; D005088:Exocrine Glands / Q000032:analysis / Q000648:ultrastructure; D007124:Immunoenzyme Techniques; D007915:Lepidoptera / Q000032:analysis*; D008870:Microtubules / Q000032:analysis; D009036:Moths / Q000032:analysis* / Q000648:ultrastructure
D004440:Ecdysone; D004441:Ecdysterone