Max-Planck-Institut für Molekulare Genetik, Abteilung Wittmann, Berlin-Dahlem, GFR.
It is well established that when E. coli 30S ribosomal subunits are irradiated with ultraviolet light under mild conditions a specific cross-link is formed between protein S7 and the 16S RNA. Methodology is presented for the analysis of the single nucleotide residue concerned in this cross-link. Firstly, the identity of the ribonuclease T1 octanucleotide attached to S7 is confirmed by a new method, which involves isolation and analysis of S7-polynucleotide complexes containing 30 -- 40 nucleotides. Secondly, the isolated S7-octanucleotide complex is digested successively with ribonuclease A, proteinase K and ribonuclease T2, and the nucleotides liberated are identified. The results show unambiguously that uridine residue number 1239 in the 16S RNA sequence is cross-linked to protein S7.
D001483:Base Sequence; D004926:Escherichia coli / Q000032:analysis*; D008970:Molecular Weight; D009698:Nucleoproteins / Q000032:analysis*; D009843:Oligoribonucleotides / Q000032:analysis; D011485:Protein Binding; D012335:RNA, Ribosomal / Q000528:radiation effects*; D012261:Ribonucleoproteins / Q000032:analysis*; D012269:Ribosomal Proteins / Q000528:radiation effects*; D012270:Ribosomes / Q000032:analysis*; D014466:Ultraviolet Rays
D009698:Nucleoproteins; D009843:Oligoribonucleotides; D012335:RNA, Ribosomal; D012261:Ribonucleoproteins; D012269:Ribosomal Proteins