Biosynthesis of murein lipoprotein in Escherichia coli: effects of 3,4-dihydroxybutyl-1-phosphonate.
The effects of 3,4-dihydroxybutyl-1-phosphonate, a four-carbon analog of sn-glycerol 3-phosphate, on the biosynthesis of the glyceryl moiety in murein lipoprotein of Escherichia coli were studied. The compound at a concentration of 55 microM strong inhibits in the incorporation of [2-3H]glycerol radioactivity into lipoprotein by virtue of its inhibition of the synthesis of phosphatidylglycerol. On the other hand, the incorporation of prelabeled [2-3H]glycerol radioactivity into lipoprotein was only partially inhbited by 3,4-dihydroxybutyl-1-phosphonate even at a much higher concentration (1 mM). These data were consistent with the postulated pathway for the biosynthesis of the lipid moiety in lipoportein: cysteine-lipoprotein + phosphatidylglycerol leads to glycerylcystein-lipoprotein + phosphatidic acid.
D001426:Bacterial Proteins / Q000096:biosynthesis*; D002072:Butylene Glycols / Q000494:pharmacology*; D002308:Cardiolipins / Q000494:pharmacology; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism; D008074:Lipoproteins / Q000096:biosynthesis*; D009943:Organophosphorus Compounds / Q000494:pharmacology; D010457:Peptidoglycan / Q000096:biosynthesis*; D010714:Phosphatidylethanolamines / Q000096:biosynthesis; D010715:Phosphatidylglycerols / Q000096:biosynthesis
D001426:Bacterial Proteins; D002072:Butylene Glycols; D002308:Cardiolipins; D008074:Lipoproteins; D009943:Organophosphorus Compounds; D010457:Peptidoglycan; D010714:Phosphatidylethanolamines; D010715:Phosphatidylglycerols; D005990:Glycerol