title: Production of a bacteriocine-like substance by group-A streptococci of M-type 4 and T-pattern 4.
abstract: A unique and characteristic bacteriocine-like inhibitor elaborated by M-type 4, T-pattern 4, group-A streptococci was isolated and partially purified. This inhibitor was found to be produced optimally in Todd-Hewitt broth; after extraction and concentration, was shown to be protein in nature, and to have a m.w. of c. 8000. It was extremely heat stable and acid tolerant, but was quickly inactivated in alkaline conditions. It could be demonstrated in cell-bound form, but 99.5% was found in culture supernates. It was specifically adsorbed by viable sensitive cells, and its mode of action was bacteristatic.
mesh_terms: D000942:Antigens, Bacterial; D001430:Bacteriocins / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology; D006358:Hot Temperature; D008970:Molecular Weight; D010447:Peptide Hydrolases / Q000494:pharmacology; D013210:Staphylococcus / Q000187:drug effects; D013297:Streptococcus pyogenes / Q000276:immunology / Q000378:metabolism*
chemical_list: D000942:Antigens, Bacterial; D001430:Bacteriocins; D010447:Peptide Hydrolases