Human cell membrane components dominant in T cell lineage: identification and characterization of human TL-like antigens.
Cell membrane components that contain beta 2-microglobulin were purified from cells of a human T cell-type leukemia cell line, HPB-ALL. They contained membrane components that have the same molecular size and the same subunit structure as HLA(A,B,C) antigens but are separable from the typical beta 2-microglobulin-containing cell membrane components, i.e., the HLA (A,B,C) antigens, by xenoantibody reagents. A sensitive radioimmunoassay was constructed for detection of the T cell membrane components. The assay revealed that the cell membrane components are expressed exclusively on cells of T cell-type leukemia cell lines among the human lymphoid cell lines tested, predominantly in thymus, among the human organs and tissues tested. They were not present on cells of human B cell-type cell lines or on cells of nonlymphoid organs and tissues. No alloantibodies directed to the T cell membrane components, the putative human homologues of mouse TL antigens, were found in any of the human tissue typing sera tested.
D000818:Animals; D000951:Antigens, Neoplasm; D001665:Binding Sites; D002460:Cell Line; D002462:Cell Membrane / Q000276:immunology; D006680:HLA Antigens; D006650:Histocompatibility Testing; D006801:Humans; D007518:Isoantibodies; D007942:Leukemia, Experimental / Q000276:immunology*; D007945:Leukemia, Lymphoid / Q000276:immunology; D009928:Organ Specificity; D010455:Peptides / Q000276:immunology; D011817:Rabbits; D013601:T-Lymphocytes / Q000276:immunology*; D013950:Thymus Gland / Q000276:immunology; D001613:beta 2-Microglobulin / Q000276:immunology
D000951:Antigens, Neoplasm; D006680:HLA Antigens; D007518:Isoantibodies; D010455:Peptides; D001613:beta 2-Microglobulin