title: Methylation patterns of tRNA at different concentrations of dimethylsulphate.
abstract: The methylation patterns produced in E. coli B tRNA by various concentrations of dimethylsulphate were found to differ with a predominant formation of 7-methylguanine and 1-methyladenine at low concentrations and of a methylated compound not yet identified at high concentrations of methylating agent. The analysis by Scatchard plot of dimethylsulphate interaction with the nucleic acid suggested the presence of high and low affinity sites.
mesh_terms: D004926:Escherichia coli; D008745:Methylation; D012343:RNA, Transfer; D013431:Sulfates / Q000494:pharmacology; D012359:tRNA Methyltransferases
chemical_list: D013431:Sulfates; D012343:RNA, Transfer; D012359:tRNA Methyltransferases