Measurement of DNA-excision repair in suspensions of freshly isolated rat hepatocytes after exposure to some carcinogenic compounds: its possible use in carcinogenicity screening.
When suspensions of freshly isolated rat hepatocytes were exposed to a number of carcinogenic compounds, it was possible to measure an increased UDS by a rapid procedure via liquid-scintillation counting. For a number of carcinogenic compounds and some of their non-carcinogenic structural analogues a good correlation between the carcinogenic property and the ability to induce UDS was demonstrable. Out of 12 carcinogenic compounds, belonging to several different chemical classes, 10 gave rise to an increased UDS, whereas only 2 compounds, the polycyclic aromatic hydrocarbons benzo[alpha]pyrene and benz[alpha]anthracene, did not. All 4 noncarcinogenic compounds tested were negative. Possibly this method can be of value as a routine screening test, in combination with other short-term test systems, thus improving the predictive value of screening in vitro with respect to carcinogenicity.
D000818:Animals; D002273:Carcinogens; D004260:DNA Repair; D004261:DNA Replication; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D005821:Genetic Techniques; D008099:Liver / Q000378:metabolism*; D008297:Male; D051381:Rats; D013936:Thymidine / Q000378:metabolism; D014316:Tritium
D002273:Carcinogens; D014316:Tritium; D013936:Thymidine