Plaque assay with protein A-coated erythrocytes for the evaluation of human immunoglobulin-secreting cells induced by pokeweed mitogen.
Cells secreting IgM, IgG and IgA were evaluated in cultures of human peripheral blood lymphocytes stimulated in vitro with pokeweed mitogen by a haemolytic plaque assay using protein A-coated erythrocytes in the presence of class-specific antisera. Kinetic study revealed that immunoglobulin-secreting cells appeared after 3 days of culture and peaked between days 5 and 7. IgM-secreting cells predominated throughout the culture period. This plaque assay is a useful and sensitive in vitro test for the evaluation of polyclonal B-cell activation in humans and may provide a good approach to the study of disturbances in the synthesis of the various classes of immunoglobulins.
D000328:Adult; D000921:Antibody-Producing Cells / Q000276:immunology; D002478:Cells, Cultured / Q000378:metabolism; D004912:Erythrocytes / Q000276:immunology; D006462:Hemolytic Plaque Technique; D006801:Humans; D007070:Immunoglobulin A / Q000096:biosynthesis; D007074:Immunoglobulin G / Q000096:biosynthesis; D007075:Immunoglobulin M / Q000096:biosynthesis; D007136:Immunoglobulins / Q000096:biosynthesis*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000276:immunology*; D011043:Pokeweed Mitogens / Q000494:pharmacology; D013205:Staphylococcal Protein A; D013997:Time Factors
D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D007136:Immunoglobulins; D011043:Pokeweed Mitogens; D013205:Staphylococcal Protein A