title: The control region of the F sex factor DNA  transfer cistrons: restriction mapping and DNA cloning.
abstract: A restriction endonuclease map of EcoRI fragment f6 of F sex factor DNA was constructed and aligned with pre-existing physical and genetic maps. Results of genetic complementation tests and analysis of proteins synthesized in minicells from PstI and BglII sub-fragment clones, or from a specific BglII fragment deletion, have allowed mapping of the locations of the origin of DNA transfer and many of the transfer genes known to lie on f6. The proteins detected account for 78% of the coding capacity of fragment f6.
mesh_terms: D002874:Chromosome Mapping; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics*; D005144:F Factor; D005796:Genes; D005816:Genetic Complementation Test; D008040:Genetic Linkage; D009876:Operon
chemical_list: D004269:DNA, Bacterial; D004262:DNA Restriction Enzymes