[Use of peroxidase and FITC labeled antibodies for express-diagnosis of influenza].
The evaluation of influenza antigen detection was done in comparative experiments on the same material during an epidemic of influenza due to A2/Victoria (H3N2) influenza virus. Cells of the nasal mucosa from 65 patients were tested at the same intervals by two methods showing no difference in the principle of the reaction: peroxidase-labeled (IP) and FITC-labeled (IF) antibody. By the latter method influenza could be diagnosed in 36% of cases, by IP in 38%, by the combination of the two in 56%. There is sufficient evidence of the specificity of IP method. This method of influenza antigen detection is as sensitive as IF (47 and 44%, respectively), however, with IP the evaluation of the results is much easier.
D000293:Adolescent; D000328:Adult; D000368:Aged; D000956:Antigens, Viral; D006801:Humans; D007124:Immunoenzyme Techniques; D009980:Influenza A virus / Q000276:immunology; D007251:Influenza, Human / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged; D009297:Nasal Mucosa / Q000276:immunology*
D000956:Antigens, Viral
Ispol'zovanie antitel, mechennykh peroksidazoĭ i FITs, dlia ékspress-diagnostiki grippa.