Interaction of Cibacron Blue F3GA with Escherichia coli DNA polymerase I and with T4 DNA polymerase.
Individual rapid procedures for the enrichment of Escherichia coli DNA polymerase I and of bacteriophage T4 DNA polymerase free of endonuclease activity are described using Blue dextran-Sepharose chromatography. The blue dye of Blue dextran-Sepharose selectively binds to the deoxynucleoside triphosphate substrate site of the E. coli but not the T4 enzyme indicating that the catalytic sites of these two enzymes which catalyze the same polymerization reaction in vitro are quite distinct.
D000873:Anthracenes / Q000494:pharmacology; D002846:Chromatography, Affinity; D003090:Coliphages / Q000201:enzymology*; D004396:Coloring Agents / Q000494:pharmacology; D004256:DNA Polymerase I / Q000302:isolation & purification* / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000302:isolation & purification* / Q000378:metabolism; D003851:Deoxyribonucleases / Q000302:isolation & purification; D004720:Endonucleases / Q000302:isolation & purification; D004926:Escherichia coli / Q000201:enzymology*; D007700:Kinetics; D014227:Triazines / Q000494:pharmacology
D000873:Anthracenes; D004396:Coloring Agents; D014227:Triazines; D004256:DNA Polymerase I; D004259:DNA-Directed DNA Polymerase; D003851:Deoxyribonucleases; D004720:Endonucleases