title: [Metabolic activities in isolated hepatocytes as a vitality parameter in the hypothermically preserved liver].
abstract: Vital hepatocytes have been isolated from an adult human liver for the first time. Vitality of the parenchymal cells was examined by trypanblue staining, morphological sppearance and energy-requiring metabolic functions such as gluconeogenesis and glycogen synthesis. Following a cold ischemic time of 6 hours metabolic activity had decreased considerably.
mesh_terms: D003080:Cold Temperature; D003584:Cytological Techniques; D005943:Gluconeogenesis; D006651:Histocytochemistry; D006801:Humans; D008099:Liver / Q000166:cytology / Q000378:metabolism*; D008112:Liver Glycogen / Q000096:biosynthesis; D009926:Organ Preservation / Q000379:methods
chemical_list: D008112:Liver Glycogen
vernacular_title: Stoffwechselaktivitäten isolierter Hepatocyten als Vitalitätsparameter in der hypotherm konservierten Leber.