title: [Rule of antibody structure. Primary structure of a human monoclonal IgA-immunoglobulin (myeloma protein Tro). VII. Purification and characterization of the disulfide bridges].
abstract: Myeloma Protein Tro has been isolated from the plasma of a myeloma patient. Monomeric IgA was separated from its polymer (by chromatography on Sephadex G-200). Both the forms were split with pepsin or cyanogen bromide and, if necessary, with thermolysin and subtilisin. The cystin-containing peptides were isolated from the hydrolysates by chromatography on Sephadex, ion-exchange columns, preparative paper chromatography, thin-layer chromatography, electrophoresis or by a combination of these methods. They were characterized by amino acid analyses and by determination of the N-terminal amino acids using the Dansyl-Edman procedure. Thus all the disulfide bridges of an IgA1 immunoglobulin could be established. The monomer has all together 48 cysteins, seven in each L- and seventeen in each H-chain; all these are covalently bonded by SS-bridges. Free SH-groups were not detected. The J-chain could only be identified serologically in the polymeric form of the protein. It is shown that the subunits of the polymers are covalently attached through either Cysl3, Cysl7 or both these residues of the H-chain.
mesh_terms: D000595:Amino Acid Sequence; D004220:Disulfides / Q000032:analysis; D006801:Humans; D007070:Immunoglobulin A; D008970:Molecular Weight; D009194:Myeloma Proteins; D010446:Peptide Fragments / Q000032:analysis; D013381:Subtilisins; D013820:Thermolysin
chemical_list: D004220:Disulfides; D007070:Immunoglobulin A; D009194:Myeloma Proteins; D010446:Peptide Fragments; D013381:Subtilisins; D013820:Thermolysin
vernacular_title: Zur Strukturregel der Antikörper. Die Primärstruktur eines monoklonalen IgA 1-Immunglobulins (Myelomprotein Tro). VII. Darstellung, Reinigung und Charakterisierung der Disulfidbrücken.