Donor- and organ-specific evaluation of antibodies eluted from canine lung allografts rejected by immunosuppressively treated and untreated recipients.
Using elution techniques, the humoral lung allograft rejection in immunosuppressively treated versus untreated recipients is analyzed at the donor organ specific level. The antibodies were evaluated quantitatively and qualitatively by elution of lung graft bound immunoglobulins and by testing the eluates against donor lung antigen using passive hemagglutination and indirect immunofluorescence. By correlating the results in those assays, a considerable amount of humoral anti-donor lung antibodies could be proved only in dogs not treated immunosuppressively, and there was no accordance with the results of direct immunofluorescence by quantification. The difference in the organ-bound antibodies between immunosuppressively treated and untreated lung grafts seems to be remarkable because of a similar mononuclear infiltration. Thus, enabling a specific improvement of some previous speculations about the lung-specific humoral alloimmune reaction, this type of rejection seems to be similar to rather stereotypical allograft rejection, but may be modified by a standard immunosuppression with methylprednisolone and azathioprine.
D000818:Animals; D000918:Antibody Specificity; D004285:Dogs; D005455:Fluorescent Antibody Technique; D006084:Graft Rejection; D006386:Hemagglutination Tests; D007518:Isoantibodies / Q000276:immunology*; D008168:Lung / Q000276:immunology; D016040:Lung Transplantation; D014181:Transplantation Immunology
D007518:Isoantibodies