title: A technique for quantitating enzyme histochemistry in adjuvant arthritic joints. I. Alkaline phosphatase.
abstract: This paper establishes a quantitative technique for measuring the percentage of enzyme-reactive cells found in adjuvant arthritic articular cartilage, synovial membrane and bone marrow. Using alkaline phosphatase histochemistry and a recently-developed method of handling hard tissues, the technique is validated, the inherent variabilities determined and the existence of intra-articular lesions established. The technique now allows for a precise in situ evaluation of adjuvant arthritic drug inhibition by measuring enzyme-reactive cell alterations within the joint tissue most affected by the arthritis.
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D001168:Arthritis / Q000201:enzymology*; D001169:Arthritis, Experimental / Q000201:enzymology* / Q000473:pathology; D001853:Bone Marrow / Q000201:enzymology; D002358:Cartilage, Articular / Q000201:enzymology; D006651:Histocytochemistry; D006652:Histological Techniques; D008297:Male; D008722:Methods; D051381:Rats; D013583:Synovial Membrane / Q000201:enzymology
chemical_list: D000469:Alkaline Phosphatase