Immunogenicity of fresh and preserved cortical and cancellous allogeneic bone grafts as tested by modified migration inhibition test in mice.
Mice were grafted with allogeneic compact and cancellous bone. The bone used was fresh and preserved by either lyophilization or deep freezing. Preserved grafts were radiosterilized by a dose of 3.3 MRads. The immunization of recipients was assessed by a modified migration inhibition test based on migration of cells from three-dimensional clot culture. Both preservation methods seem to change bone immunogenicity and enhancement of migration instead of inhibition occurring in the case of fresh bone was noted.
D000818:Animals; D016025:Bone Transplantation; D001842:Bone and Bones / Q000276:immunology*; D002464:Cell Migration Inhibition; D005260:Female; D007111:Immunity, Cellular; D051379:Mice; D011309:Preservation, Biological; D014184:Transplantation, Homologous