Multiparameter kinetic analysis of killer cell initiation by using immune RNA.
By treating nonsensitized C57BL/6J spleen derived lymphocytes with EL-4 tumor cell directed xenogeneic extracted RNA we were able to monitor early changes in cellular DNA content by flow cytometric (FCM) analysis and 3H-thymidine uptake. These kinetic parameters were correlated with cell mediated cytotoxicity which appeared as early as 8 hr after activation as measured by release of chromium-51 from labeled EL-4 target cells. Flow cytometric analysis and 3H-thymidine uptake data shown peak S phase activity at 72 hr. Maximum cytotoxicity was observed at 48 hr. Cell cycle kinetic parameters were correlated with the appearance of cell mediated cytotoxicity.
D000818:Animals; D002453:Cell Cycle; D002460:Cell Line; D003584:Cytological Techniques; D003602:Cytotoxicity, Immunologic; D007942:Leukemia, Experimental; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D010783:Photometry; D012334:RNA, Neoplasm / Q000276:immunology*; D013154:Spleen / Q000166:cytology; D013936:Thymidine; D014316:Tritium
D012334:RNA, Neoplasm; D014316:Tritium; D013936:Thymidine