Restriction map, partial cloning and localization of 9S and 12S kinetoplast RNA genes on the maxicircle component of the kinetoplast DNA of Leishmania tarentolae.
We have constructed a restriction map of the maxicircle component of the kinetoplast DNA of Leishmania tarentolae for the enzymes EcoRI, Bam HI, HaeIII, HpaII, SalI, BglII and HindIII. The 9 and 12S kinetoplast RNAs were localized on this map. Two fragments of this maxicircle molecule were cloned in the bacterial plasmid, pBR322, including a 4.4 . 10(6) dalton EcoRI/BamHI fragment which contains the 9 and 12S RNA genes.
D000818:Animals; D004269:DNA, Bacterial / Q000235:genetics; D004270:DNA, Circular / Q000235:genetics*; D004274:DNA, Recombinant / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D005796:Genes; D008040:Genetic Linkage; D007891:Leishmania / Q000235:genetics*; D010957:Plasmids; D012313:RNA / Q000235:genetics*
D004269:DNA, Bacterial; D004270:DNA, Circular; D004274:DNA, Recombinant; D012313:RNA