The use of immobilized ligands and [125I]protein a for immunoassays of thromboxane B2, prostaglandin D2, 13,14-dihydro-prostaglandin E2, 5,6-dihydro-prostaglandin I2, 6-keto-prostaglandin F1 alpha, 15-hydroxy-9 alpha, 11 alpha(epoxymethano)prosta-5,13-dienoic acid and 15-hydroxy-11 alpha,9 alpha(epoxymethano)prosta-5,13-dienoic acid.
Immunoassays were developed for quantitative determination of thromboxane B2, prostaglandin D2, 13,14-dihydro-prostaglandin E2, 5,6-dihydro-prostaglandin I2, 6-keto-prostaglandin F1 alpha, 15-hydroxy-9 alpha, 11 alpha (epoxymethano) prosta-5, 13-dienoic acid and 15-hydroxy-11 alpha, 9 alpha (epoxymethano) prosta-5,13-dienoic acid. Ligands immobilized by covalent linkage to a solid support, bound homologous rabbit antibodies. [125I] Protein A was used to measure the bound IgG antibody. Increments of homologous and heterologous fluid-phase ligand completed with solid-phase ligand for antibody and resulted in decreasing amounts of bound [125I]-Protein A. The serologic specificity for each immune system was determined. Immunoassays for thromboxane B2, 6-keto-prostaglandin F1 alpha, and 5,6-dihydro-prostaglandin I2 were used to identify their respective homologous ligands that were separated by normal phase and reversed phase high pressure liquid chromatography.
D019796:15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid; D015121:6-Ketoprostaglandin F1 alpha; D000818:Animals; D000918:Antibody Specificity; D000937:Antigen-Antibody Reactions; D015232:Dinoprostone / Q000031:analogs & derivatives*; D011464:Epoprostenol / Q000031:analogs & derivatives; D008024:Ligands; D011450:Prostaglandin Endoperoxides, Synthetic / Q000032:analysis; D011453:Prostaglandins / Q000032:analysis*; D011457:Prostaglandins D / Q000097:blood; D011459:Prostaglandins E, Synthetic / Q000032:analysis; D011460:Prostaglandins F; D011465:Prostaglandins, Synthetic / Q000032:analysis*; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay; D013205:Staphylococcal Protein A; D013929:Thromboxane B2 / Q000097:blood
D008024:Ligands; D011450:Prostaglandin Endoperoxides, Synthetic; D011453:Prostaglandins; D011457:Prostaglandins D; D011459:Prostaglandins E, Synthetic; D011460:Prostaglandins F; D011465:Prostaglandins, Synthetic; D013205:Staphylococcal Protein A; C025395:13,14-dihydroprostaglandin E2; D013929:Thromboxane B2; D015121:6-Ketoprostaglandin F1 alpha; C022228:5,6-dihydroprostacyclin; D019796:15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid; D011464:Epoprostenol; D015232:Dinoprostone