The application of antibody coated polyacrylamide gel (immunobead) in the NBT test for the detection of ingestion and killing functions of human neutrophils.
An improved assay for the simultaneous assessment of phagocytic uptake (via immunobeads) and metabolic integrity (via NBT dye reduction) in neutrophils is described. Normal human neutrophils exhibited ingestion and dye reduction, whereas neutrophils from newborn cord blood showed ingestion with slight reduction and neutrophils from patients with chronic granulomatous disease displayed hyper-ingestion and no subsequent dye reduction.
D000178:Acrylamides; D000906:Antibodies; D005562:Formazans; D005782:Gels; D006105:Granulomatous Disease, Chronic / Q000276:immunology; D006801:Humans; D007158:Immunologic Techniques; D007231:Infant, Newborn; D009504:Neutrophils / Q000276:immunology*; D009580:Nitroblue Tetrazolium / Q000378:metabolism; D010587:Phagocytosis; D013778:Tetrazolium Salts
D000178:Acrylamides; D000906:Antibodies; D005562:Formazans; D005782:Gels; D013778:Tetrazolium Salts; D009580:Nitroblue Tetrazolium