Transcription and processing of cloned yeast tyrosine tRNA genes microinjected into frog oocytes.
Xenopus oocytes transcribe cloned yeast tyrosine tRNA genes and process the RNA. The processing includes base modifications, addition of the CCA end, and splicing of the intervening sequence. The primary transcript has a 5' leader extension which varies considerably in seuqence and in length between different tRNATyr loci, but is nevertheless present in all of them.
D000818:Animals; D001483:Base Sequence; D004274:DNA, Recombinant; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D005260:Female; D005796:Genes; D009694:Nucleic Acid Precursors / Q000235:genetics; D009865:Oocytes / Q000378:metabolism; D010957:Plasmids; D011130:RNA Ligase (ATP) / Q000378:metabolism; D012343:RNA, Transfer / Q000235:genetics*; D012260:Ribonucleases / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics; D014158:Transcription, Genetic; D014443:Tyrosine; D014981:Xenopus / Q000235:genetics*
D004274:DNA, Recombinant; D009694:Nucleic Acid Precursors; D014443:Tyrosine; D012343:RNA, Transfer; D012321:DNA-Directed RNA Polymerases; D012260:Ribonucleases; D011130:RNA Ligase (ATP)