Indication of antigens and antibodies using the reaction of compound immune adsorption and reactivation of the phage.
A method of determining antibodies by their adsorption on large-pore or surface immunosorbents with subsequent treatment of the carrier with anti-immunoglobulin serum and antiphage serum isologous to the antibodies and then with the bacteriophage has been presented. The adsorbed virions are split off by means of papain-induced hydrolysis of the antibody complex. The antigens are determined by the reaction of phage fixing inhibition. The method permits to determine small amounts of antibodies to proteins, haptenes and cells with objective calculation of results.
D000907:Antibodies, Bacterial / Q000302:isolation & purification*; D000936:Antigen-Antibody Complex; D000942:Antigens, Bacterial / Q000302:isolation & purification*; D001435:Bacteriophages / Q000276:immunology*; D003470:Culture Media; D004926:Escherichia coli / Q000276:immunology; D007106:Immune Sera; D007136:Immunoglobulins / Q000302:isolation & purification
D000907:Antibodies, Bacterial; D000936:Antigen-Antibody Complex; D000942:Antigens, Bacterial; D003470:Culture Media; D007106:Immune Sera; D007136:Immunoglobulins