title: The binding of LPS to the lymphocyte surface.
abstract: Bacterial lipopolysaccharide (LPS) from Escherichia coli labelled with tritium has been used to follow the binding of LPS to lymphocytes. Binding to cells rose to a maximum 2-7 min after addition of [3H]-LPS, followed by loss of [3H]-LPS from cells, reducing to about 10% of the peak level at 20-30 min. Peripheral blood lymphocytes, mesenteric lymph node and thymus cells of the pig and CBA, C3H/He and C3H/HeJ mouse spleen cells all bound [3H]-LPS transiently at similar levels. It is concluded that this type of LPS binding cannot be solely responsible for the preferential stimulation of B cells by LPS.
mesh_terms: D000818:Animals; D001386:Azides / Q000494:pharmacology; D001402:B-Lymphocytes / Q000276:immunology; D002462:Cell Membrane / Q000378:metabolism; D004926:Escherichia coli; D008070:Lipopolysaccharides / Q000378:metabolism*; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008809:Mice, Inbred C3H; D008808:Mice, Inbred CBA; D013154:Spleen / Q000166:cytology / Q000378:metabolism; D013552:Swine; D013950:Thymus Gland / Q000166:cytology / Q000378:metabolism; D013997:Time Factors
chemical_list: D001386:Azides; D008070:Lipopolysaccharides