[Slightly active Actinomyces streptomycini (Streptomyces griseus) mutants having a protein in the biosynthesis of the streptidine portion of the streptomycin molecule].
Twelve mutants having a block in biosynthesis of the streptidine part of the streptomyciu molecule were selected under the effect of nitrozomethylbiuret. These 12 strains responded by an increase in the level of the antibiotic production to the addition of streptidine to the cultivation medium. The complementation analysis showed that every streptidine-dependent mutant interacted at least with 2 other mutants. On the basis of the data obtained it is possible to conclude that all 12 mutants had blocks in streptidine biosynthesis but at different stages of this complicated process.
D001426:Bacterial Proteins / Q000378:metabolism*; D003511:Cyclohexanols / Q000138:chemical synthesis; D005816:Genetic Complementation Test; D006146:Guanidines / Q000138:chemical synthesis*; D006595:Hexosamines; D008770:Methylnitrosourea / Q000494:pharmacology; D009154:Mutation / Q000187:drug effects; D013305:Streptomyces griseus / Q000235:genetics / Q000378:metabolism*; D013307:Streptomycin / Q000096:biosynthesis*
D001426:Bacterial Proteins; D003511:Cyclohexanols; D006146:Guanidines; D006595:Hexosamines; D008770:Methylnitrosourea; C005733:streptidine; D013307:Streptomycin
Maloaktivnye mutanty Actinomyces streptomycini (Streptomyces griseus), imeiushchie belok v biosinteze streptidinovoĭ chasti molekuly streptomitsina.