[Fast immunochemical assay of proteins with Gemsaec centrifugal analyser. Application to serum transferrin and IgA assay].
An automated method for measurement of specific proteins on a centrifugal analyser is reported. Based on the immunoprecipitin turbidimetric reaction enhanced by polyethylene-glycol, the technique, simple and fast, gives precise and accurate results with following cautions: the antigen and antibody concentrations must be carefully chosen according to defined specific antiserum, and two dilutions of each serum sample must be assayed to control quite satisfactory determination and exhibit any antigen excess error. Large series of serum transferrin and IgA assays were studied and compared with radial immunodiffusion and continuous-flow immunonephelometric method: good correlations prove the value of the reported method.
D001322:Autoanalysis / Q000379:methods*; D002498:Centrifugation; D003124:Colorimetry; D006801:Humans; D007106:Immune Sera; D007118:Immunoassay / Q000379:methods; D005779:Immunodiffusion; D007070:Immunoglobulin A / Q000032:analysis*; D009391:Nephelometry and Turbidimetry; D011233:Precipitin Tests / Q000379:methods; D014168:Transferrin / Q000032:analysis*
D007106:Immune Sera; D007070:Immunoglobulin A; D014168:Transferrin
Dosage immunochimique rapide des protéines sur analyseur centrifuge gemsaec. Application au dosage de la transferrine et des IgA du sérum sanguin.