Membrane-bound nucleotidase of Bacillus cereus.
A membrane-bound nucleotidase of Bacillus cereus T was solubilized by digestion with trypsin and subsequently purified more than 300-fold. The purified nucleotidase was most active on ribonucleoside 5'-monophosphates and was slightly less active (40 to 60%) on deoxyribonucleoside 5'-monophosphates and ribonucleoside 3'-monophosphates. In addition to hydrolytic activity, the nucleotidase preparation possessed phosphotransferase activity by which phosphate is transferred from a phosphate donor to the 5' position of nucleosides.
D001409:Bacillus cereus / Q000201:enzymology*; D002462:Cell Membrane / Q000201:enzymology; D003854:Deoxyribonucleotides / Q000378:metabolism; D008970:Molecular Weight; D009708:Nucleotidases / Q000302:isolation & purification* / Q000378:metabolism; D012265:Ribonucleotides / Q000378:metabolism; D013379:Substrate Specificity
D003854:Deoxyribonucleotides; D012265:Ribonucleotides; D009708:Nucleotidases