A practical radioimmunoassay of thyroglobulin.
We describe a reliable and convenient double-antibody radioimmunoassay for human thyroglobulin that can be performed within two days. Analytical recoveries were quantitative and immunological equivalence between several sources of human thyroglobulin was demonstrated. Cross reactivity with either thyroxine or triiodothyronine was insignificant. The between-day coefficient of variation in the 5--100 mug/liter range was 10%. The range observed for 68 normal individuals was 2--61 microgram/liter.
D000328:Adult; D000918:Antibody Specificity; D003429:Cross Reactions; D004306:Dose-Response Relationship, Immunologic; D005260:Female; D006801:Humans; D007106:Immune Sera; D005779:Immunodiffusion; D008297:Male; D008875:Middle Aged; D011863:Radioimmunoassay / Q000379:methods; D012016:Reference Values; D013954:Thyroglobulin / Q000032:analysis* / Q000097:blood / Q000276:immunology
D007106:Immune Sera; D013954:Thyroglobulin