A passive hemagglutination test for detecting bovine serum albumin antibodies.
A microtiter passive hemagglutination test is described for detecting and titrating antibodies in chickens. Chicken erythrocytes are coated with antigen (bovine serum albumin = BSA) in the presence of 0.25% glutaraldehyde. Such sensitized erythrocytes are agglutinated in the presence of antibodies against BSA. The sensitivity and specificity of the test are not affected by salt concentrations in the range of 0.9-9%. This test measures the course of antibody response in chickens immunized with BSA.
D000818:Animals; D000906:Antibodies / Q000032:analysis*; D002645:Chickens / Q000276:immunology*; D004912:Erythrocytes / Q000187:drug effects / Q000276:immunology; D005976:Glutaral / Q000494:pharmacology; D006386:Hemagglutination Tests; D012710:Serum Albumin, Bovine / Q000276:immunology*
D000906:Antibodies; D012710:Serum Albumin, Bovine; D005976:Glutaral