Buoyant density fractionation of Drosophila melanogaster chromatin.
Buoyant density fractionation of Drosophila melanogaster chromatin utilizing low molecular weight molecules such as actinomycin-D to induce changes in buoyant density has been investigated. Fractions of chromatin containing identifiable repeated DNA sequences could be isolated using actinomycin-D as the selective agent. Protein displacement from the chromatin complex was found to be a prerequisite for the observed buoyant density changes.
D000818:Animals; D002499:Centrifugation, Density Gradient; D002843:Chromatin / Q000648:ultrastructure; D004247:DNA / Q000032:analysis; D003609:Dactinomycin; D004331:Drosophila melanogaster; D007700:Kinetics; D009691:Nucleic Acid Denaturation; D013696:Temperature
D002843:Chromatin; D003609:Dactinomycin; D004247:DNA