title: Establishment and characteristics of three mouse cell lines from embryos of different inbred strains.
abstract: Three cell lines were established from embryos of DDD, AKR, and C3H mouse strains based on "3T3" transfer schedule. These cell lines were designated as Y-DD, Y-AK, and Y-CH, Y-DD and Y-CH showed a gradually increasing growth rate during the course of their establishment, whereas Y-ak indicated a constant growth rate, a low level of saturation density, and a tetraploid range of chromosome mode. In transformation experiment, control cultures of Y-DD and Y-CH already lost a density-dependent inhibition, but Y-AK was found to be very sensitive to density-dependent inhibition. Y-AK showed a higher activity in benzo[a]pyrene metabolism (4100 pmol/10(6) cells/24 hr) than Y-DD and Y-CH (700 and 2600 pmol/10(6) cells/24 hr).
mesh_terms: D015112:4-Nitroquinoline-1-oxide; D015127:9,10-Dimethyl-1,2-benzanthracene; D000818:Animals; D001580:Benzopyrenes / Q000378:metabolism; D002455:Cell Division; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000187:drug effects; D003260:Contact Inhibition; D007621:Karyotyping; D008769:Methylnitronitrosoguanidine; D051379:Mice; D008806:Mice, Inbred AKR / Q000196:embryology; D008809:Mice, Inbred C3H / Q000196:embryology; D008815:Mice, Inbred Strains / Q000196:embryology*; D013045:Species Specificity
chemical_list: D001580:Benzopyrenes; D008769:Methylnitronitrosoguanidine; D015112:4-Nitroquinoline-1-oxide; D015127:9,10-Dimethyl-1,2-benzanthracene