[Production of Friend virus particles at the surface of cells synchronized by Ficoll gradient centrifugation].
Murine cells continuously producing Friend viral complex were synchronized by Ficoll gradient centrifugation. Cells were isolated from different parts of the gradient and counts of budding particles were performed on ultra-thin sections. No significant differences were noticed, suggesting that viral release is not related to cell cycle.
D002453:Cell Cycle; D002478:Cells, Cultured; D002499:Centrifugation, Density Gradient; D004279:DNA, Viral / Q000096:biosynthesis*; D005362:Ficoll; D005622:Friend murine leukemia virus; D008854:Microscopy, Electron; D014776:Virus Cultivation
D004279:DNA, Viral; D005362:Ficoll
Production de particules du virus de Friend à la surface de cellules synchronisées par centrifugation en gradient de Ficoll.