Improved procedure of purification of inorganic pyrophosphatase from Bacillus stearothermophilus.
An improved method for isolation of inorganic pyrophosphatase (EC 3.6.1.1) from Bacillus stearothermophilus is described. The enzyme was purified to more than 90% after two chromatographic steps. A molecular weight of 140,000 daltons was estimated by density gradient centrifugation. The isoelectric point was found to be 4.0.
D002499:Centrifugation, Density Gradient; D002845:Chromatography; D001411:Geobacillus stearothermophilus / Q000201:enzymology*; D008970:Molecular Weight; D011755:Pyrophosphatases / Q000302:isolation & purification*
D011755:Pyrophosphatases