Partial purification and characterization of a uracil DNA N-glycosidase from Bacillus subtilis.
A uracil specific DNA N-glycosidase activity has been partially purified from crude extracts of Bacillus subtilis. The enzyme has a molecular weight of approximately 24 000 with no subunit structure. It has no requirement for any known cofactors but is inhibited in the presence of Co2+, Fe2+, or Zn2+. The enzyme is specific for uracil in single- and double-stranded deoxyribonucleopolymers and does not release free uracil from RNA or from poly(rU):poly(dA). In addition, neither Udr, dUMP, nor dUTP is recognized as substrate. The enzyme will attack small poly(dU) oligomers but the minimum size recognized as substrate is (pU)4. This enzyme may have a role in the repair (by base excision) or uracil in DNA arising either by incorporation during DNA synthesis or by deamination of cytosine in DNA.
D001412:Bacillus subtilis / Q000201:enzymology*; D003035:Cobalt / Q000494:pharmacology; D004247:DNA / Q000378:metabolism*; D007501:Iron / Q000494:pharmacology; D007700:Kinetics; D008970:Molecular Weight; D009699:N-Glycosyl Hydrolases / Q000302:isolation & purification / Q000378:metabolism; D009838:Oligodeoxyribonucleotides / Q000378:metabolism; D011072:Poly U / Q000378:metabolism; D011089:Polydeoxyribonucleotides / Q000378:metabolism; D013329:Structure-Activity Relationship; D014498:Uracil / Q000378:metabolism*; D015032:Zinc / Q000494:pharmacology
D009838:Oligodeoxyribonucleotides; D011089:Polydeoxyribonucleotides; D011072:Poly U; D003035:Cobalt; D014498:Uracil; D004247:DNA; D007501:Iron; D009699:N-Glycosyl Hydrolases; D015032:Zinc