Detection of the in vivo conversion of 2-pyrrolidinone to gamma-aminobutyric acid in mouse brain.
Labeled gamma-aminobutyric acid was detected in mouse brain following intravenous injections of deuterium labeled 2-pyrrolidinone. [2H6]Pyrrolidinone was prepared by the reduction of [2H4]succinimide with lithium aluminum deuteride. Quantification was accomplished by a gas chromatography mass spectrometry assay method. gamma-Aminobutyric acid and internal standard, 5-aminovaleric acid, were converted to volatile derivatives by treatment with N,N-dimethylformamide dimethyl acetal. Quantitative estimates were derived from peak area measurements obtained from monitoring the parent ions of the gamma-aminobutyric acid and internal standard derivatives by repetitive scanning during the GC run. The conversion of pyrrolidinone to gamma-aminobutyric acid may provide a method for labeling central gamma-aminobutyric acid pools.
D000818:Animals; D001921:Brain / Q000378:metabolism; D001923:Brain Chemistry; D002849:Chromatography, Gas; D003903:Deuterium; D007553:Isotope Labeling; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D051379:Mice; D011760:Pyrrolidinones / Q000138:chemical synthesis / Q000378:metabolism* / Q000494:pharmacology; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D011760:Pyrrolidinones; D005680:gamma-Aminobutyric Acid; D003903:Deuterium