Fate of juvenile hormone in mammalian cell culture.
The metabolism of juvenile hormone (JH) I has been examined in fetal mouse liver cells maintained in culture. Diffusion of the hormone into the cells appears to be passive. The hormone is metabolized essentially to organic-soluble metabolites (diol ester, diol acid and acid) by the action of epoxide hydrase and carboxylesterases. Conjugative reactions play a minor role, less than 3% of the hormone being excreted as conjugates (glucuronides, sulfates and mercapturic acid). About 0.8% of the cellular radioactivity is bound to macromolecules, mainly those of nuclear and mitochondrial origin. Metyrapone and SKF 525-A inhibit covalent binding of the hormone to cytoplasmic macromolecules, which suggests participation of the cytochrome P-450 system in covalent binding of the hormone.
D000818:Animals; D001711:Biotransformation; D002478:Cells, Cultured; D007605:Juvenile Hormones / Q000378:metabolism*; D008099:Liver / Q000196:embryology / Q000378:metabolism; D051379:Mice; D013997:Time Factors
D007605:Juvenile Hormones