title: Molecular changes in the membranes of mouse erythroid cells accompanying differentiation.
abstract: The development of the mouse erythroblast to a mature erythrocyte is accompanied by changes in the composition and properties of the plasma membranes of these cells. Using double fluorescence techniques, we have simultaneously determined the distribution of lectin receptors and spectrin on the membranes of these cells. The lateral mobility of the lectin receptors in the membranes decreases as differentiation proceeds, and this is accompanied by an increasing concentration of spectrin associated with the membranes. The most significant concentration of spectrin occurs, however, during the enucleation of the late erythroblast, where we observe a complete segregation of the spectrin to the incipient reticulocyte, as well as a previously observed enrichment of receptors for concanavalin A into the plasma membrane surrounding the extruding nucleus. On the basis of these and other observations, we explore the possible molecular mechanisms involved in erythroblast enucleation and the role of spectrin in the regulation of protein mobility in erythroid cell membranes.
mesh_terms: D000199:Actins / Q000378:metabolism*; D001854:Bone Marrow Cells; D002467:Cell Nucleus / Q000378:metabolism; D004910:Erythrocyte Membrane / Q000378:metabolism*; D004912:Erythrocytes / Q000378:metabolism*; D004920:Erythropoiesis; D005089:Exocytosis; D007152:Immunologic Capping; D037102:Lectins; D008565:Membrane Proteins / Q000378:metabolism*; D011952:Receptors, Concanavalin A / Q000378:metabolism*; D011955:Receptors, Drug / Q000378:metabolism*; D012156:Reticulocytes / Q000378:metabolism; D013049:Spectrin / Q000378:metabolism*
chemical_list: D000199:Actins; D037102:Lectins; D008565:Membrane Proteins; D011952:Receptors, Concanavalin A; D011955:Receptors, Drug; D013049:Spectrin