Architecture of the outer membrane of Escherichia coli K12. II. Freeze fracture morphology of wild type and mutant strains.
Freeze fracturing electron microscopy of Escherichia coli K12 cells showed that the outer fracture face of the outer membrane is densily occupied with particles. On the inner fracture face of the outer membrane, pits are visible, which are probably complementary to the particles at opposite fracture face. This observation suggests that the particles are micelle-like. In some mutants which lack one or more major outer membrane proteins the density of particles is reduced. The loss of protein d appeared to a prerequisite for this phenomenon. However, mutants which lack all glucose and heptose-bound phosphate in their lipopolysaccharide also have a reduction in particle density whereas, the amount of protein d is normal. Moreover, loss of lipopolysaccharide by EDTA treatment also caused a reduction in the density of particles. From these results it is hypothesized that the particles consist of lipopolysaccharide aggregates stabilized by divalent cations and probably complexed with protein and/or phospholipid.
D001426:Bacterial Proteins / Q000032:analysis; D002462:Cell Membrane / Q000648:ultrastructure*; D004492:Edetic Acid / Q000494:pharmacology; D004926:Escherichia coli / Q000648:ultrastructure*; D005614:Freeze Fracturing; D008070:Lipopolysaccharides / Q000032:analysis; D008565:Membrane Proteins / Q000032:analysis; D009154:Mutation
D001426:Bacterial Proteins; D008070:Lipopolysaccharides; D008565:Membrane Proteins; D004492:Edetic Acid