Electron microscope analysis of mouse and rabbit globin and immunoglobulin gene sequences.
Mouse and rabbit globin and immunoglobulin gene sequences, which had been synthesized in vitro from eukaryotic mRNAs and inserted into plasmids, have been examined in the electron microscope. The size of the inserted beta rabbit and alpha and beta mouse globin DNA sequences has been estimated as 620 base pairs while the size of the inserted alpha rabbit globin DNA sequences was found to be about 490 base pairs. Heteroduplex analysis has revealed no structural abnormalities at the insertion sites of the chimeric plasmids except in the case of a plasmid containing an immunoglobulin light chain gene sequence of about 830 bases, in which 3 kb deletion adjacent to the insertion site was observed.
D000818:Animals; D001483:Base Sequence; D004269:DNA, Bacterial; D004274:DNA, Recombinant; D005796:Genes; D005914:Globins / Q000235:genetics*; D007147:Immunoglobulin Light Chains / Q000235:genetics*; D066298:In Vitro Techniques; D051379:Mice; D008854:Microscopy, Electron; D009690:Nucleic Acid Conformation; D010957:Plasmids; D011817:Rabbits
D004269:DNA, Bacterial; D004274:DNA, Recombinant; D007147:Immunoglobulin Light Chains; D005914:Globins