title: Biosynthesis of a prostaglandin by a plant enzyme.
abstract: Lipoxygenase-2 from soybeans catalyzes the oxygenation of arachidonic acid to form significant amounts of a prostaglandin product. Results obtained with the dithionite-reduced derivatized product upon combined gas chromatography-mass spectroscopy are consistent with structures of the stereoisomers of 9,11,15-trihydroxyprosta-5,13-dienoic acid. A portion of the material formed by reduction of the enzyme product with dithionite reacts positively in a radioimmunoassay against rabbit anti-prostaglandin F2alpha antibody. This appears to be the first report of the synthesis of a prostaglandin by a non-animal enzyme.
mesh_terms: D001095:Arachidonic Acids / Q000378:metabolism; D008084:Lipoxygenase / Q000378:metabolism*; D013058:Mass Spectrometry; D010944:Plants / Q000201:enzymology*; D011460:Prostaglandins F / Q000096:biosynthesis*; D013025:Glycine max
chemical_list: D001095:Arachidonic Acids; D011460:Prostaglandins F; D008084:Lipoxygenase