[Surface immunoglobulins (SmIg) in human B-lymphocytes: effect of enzymatic digestion].
In order to determine the number of circulating B lymphocytes which can synthetize their surface membrane immunoglobulins, we have evaluated by immunofluorescence their presence before and after enzymatic exposure to pronase. After pronase treatment the majority of lymphocytes carrying SmIg has been disappeared (from 7.8% to 1.9%; p < .0.0005). After 18 hours incubaton only a small proportion (4.6%) of B lymphocytes risynthetized their SmIg. This observation support the hypothesis that a subset of lymphocytes present in the peripheral blood carries on its membrane only cytophilic SmIg and therefore may interfere in the determination of the number of B lymphocytes.
D000328:Adult; D001402:B-Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D008297:Male; D011402:Pronase / Q000494:pharmacology*; D011947:Receptors, Antigen, B-Cell / Q000096:biosynthesis* / Q000378:metabolism
D011947:Receptors, Antigen, B-Cell; D011402:Pronase
Immunoglobuline di superficie (SmIg) nei linfociti B nell'uomo: effetto della digestione enzimatica.