title: Nucleotide sequence of threonine tRNA from Bacillus subtilis.
abstract: A threonine tRNA was purified from Bacillus subtilis W168 by a combined use of column chromatographic systems. The nucleotide sequence was determined to be pG-C-C-G-G-U-G-U-A-G-C-U-C-A-A-U-D-G-G-D(U)-A-G-A-G-C-A-A-C-U-G-A-C-U-mo5U-G-U-t6A-A-psi-C-A-G-U-A-G-m7G-U-U-G-G-G-G-G-T-psi-C-A-A-G-U-C-C-U-C-U-U-G-C-C-G-G-C-A-C-C-AOH, where about 40 % of D20 remained unmodified as U20. It consists of 76 nucleotides including a new minor nucleoside, 5-methoxyuridine (mo5U), which occupies the wobble position of anticodon.
mesh_terms: D000926:Anticodon / Q000032:analysis; D001412:Bacillus subtilis / Q000032:analysis; D001483:Base Sequence; D012329:RNA, Bacterial / Q000302:isolation & purification; D012343:RNA, Transfer / Q000302:isolation & purification*; D013912:Threonine; D014529:Uridine / Q000031:analogs & derivatives
chemical_list: D000926:Anticodon; D012329:RNA, Bacterial; D013912:Threonine; C016562:5-methoxyuridine; D012343:RNA, Transfer; D014529:Uridine