Inducible resistance to D-cycloserine in Bacillus subtilis 168.
Resistance to d-cycloserine could be induced in Bacillus subtilis 168 by sublethal concentrations of d-cycloserine. Sensitivity to the antibiotic could be regained by growth in the absence of d-cycloserine. The bactericidal activity of d-cycloserine apparently was not altered by resistant cells, and peptidoglycan synthesis was still inhibited by d-cycloserine in resistant cells. The d-cycloserine resistance apparently resulted from a decreased uptake of the antibiotic. The decrease in d-cycloserine transport could be prevented by simultaneous treatment of the cells with rifampin and d-cycloserine. d-Cycloserine was transported by the same system as glycine in B. subtilis. d-Cycloserine was able to exchange for intracellular glycine in both sensitive and resistant cells, suggesting that d-cycloserine is not excluded from the cell in resistant cultures.
D001412:Bacillus subtilis / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D001693:Biological Transport, Active / Q000187:drug effects; D003523:Cycloserine / Q000378:metabolism / Q000494:pharmacology*; D004352:Drug Resistance, Microbial; D005998:Glycine / Q000378:metabolism; D010457:Peptidoglycan / Q000378:metabolism; D013997:Time Factors
D010457:Peptidoglycan; D003523:Cycloserine; D005998:Glycine