Plant protoplast agglutination by artificial carbohydrate antigens.
The existence of beta-lectins on protoplast surfaces is confirmed by the agglutination of protoplasts by those Yariv antigens that have sugar specificities which also interact with isolated beta-lectins. Agglutination by beta-maltosyl but not by beta-D-mannosyl Yariv antigens is used to identify some of the structural features required of the ligand for beta-lectin binding. Inhibition of agglutination by phenolic glycosides and the effect of protoplast fixation are also investigated.
D000371:Agglutination / Q000187:drug effects; D000941:Antigens; D005976:Glutaral / Q000494:pharmacology; D006027:Glycosides / Q000276:immunology / Q000494:pharmacology; D066298:In Vitro Techniques; D037102:Lectins; D011523:Protoplasts / Q000276:immunology*
D000941:Antigens; D006027:Glycosides; D037102:Lectins; D005976:Glutaral