The interactions of the separated strands of satellite DNAs with other DNAs: 1. Conditions for associations of the alpha-satellite of the guinea pig with heterologous double-stranded DNAs.
The separated H- and L-strands of the alpha-satellite of the guinea pig, Cavea porcellus, recovered from centrifugation in alkaline CsC1 gradients, from complexes with 7 different double-stranded (ds) DNAs including those of 1 bacteriophage, 2 prokaryotes, 2 invertebrates and 2 mammals. The complexes are not artifacts due to in vitro labeling of the satellite, methods of collection, the presence of divalent cations, or the fact that trace amounts of single-stranded (ss) DNAs are used. More complex dsDNAs, such as that recovered from nicked RF M13, do not associate with dsDNAs.
D000818:Animals; D003386:Brachyura; D003090:Coliphages; D004247:DNA / Q000378:metabolism*; D004269:DNA, Bacterial / Q000378:metabolism; D004276:DNA, Satellite / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000378:metabolism*; D004279:DNA, Viral / Q000378:metabolism; D003851:Deoxyribonucleases; D004492:Edetic Acid / Q000494:pharmacology; D004926:Escherichia coli; D006168:Guinea Pigs / Q000378:metabolism*; D006358:Hot Temperature; D006801:Humans; D008837:Micrococcus; D013045:Species Specificity; D013329:Structure-Activity Relationship; D013950:Thymus Gland
D004269:DNA, Bacterial; D004276:DNA, Satellite; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D004247:DNA; D004492:Edetic Acid; D003851:Deoxyribonucleases