title: Automated scanning of 14C on polyacrylamide gel.
abstract: Cylindrical polyacrylamide gels were automatically analyzed for radioactive 14C using a commercially available radiochromatogram scanner. This method was capable of detecting peaks of [14C]protein containing 50 000--1 000 000 cpm. Under optimal conditions, gels could be scanned at a rate of 1 cm per min, allowing for analysis of 20--30 gels per day.
mesh_terms: D001322:Autoanalysis; D002250:Carbon Radioisotopes; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods; D004921:Erythropoietin / Q000032:analysis; D008832:Microchemistry; D011506:Proteins / Q000032:analysis*; D011865:Radioisotope Dilution Technique
chemical_list: D002250:Carbon Radioisotopes; D011506:Proteins; D004921:Erythropoietin