title: Artificial insemination homologous with oligospermic semen separated on albumin columns.
abstract: Semen samples obtained from 27 infertile men were separated on human serum albumin (HSA) columns prior to artificial homologous insemination (AIH). The columns contained either a single 7.5% HSA layer or two 17.5% and 7.5% HSA layers. Separated specimens were free of seminal debris, had significantly improved motility and progressive drive, and had a decreased percentage of abnormal forms. The total sperm count was also significantly decreased after separation, especially with the two-layer technique. No conceptions occurred in 21 couples when two-layer separation was used, but four pregnancies in 12 couples resulted from AIH with semen separated on the single-layer column. The mean total count of motile sperm obtained for AIH was 5 million with the two-layer and 31 million with the one-layer technique.
mesh_terms: D000328:Adult; D002469:Cell Separation / Q000379:methods; D005260:Female; D006801:Humans; D007315:Insemination, Artificial; D007317:Insemination, Artificial, Homologous; D008297:Male; D009845:Oligospermia; D011247:Pregnancy; D012661:Semen; D012709:Serum Albumin; D013081:Sperm Motility; D013094:Spermatozoa
chemical_list: D012709:Serum Albumin