title: Transformation of Escherichia coli and Bacillus subtilis with a hybrid plasmid molecule.
abstract: A hybrid molecule constructed from Escherichia coli plasmid pMB9 and a fragment of Bacillus subtilis 168 deoxyribonucleic acid functions in cells of leu-E. coli, converting them to leucine prototrophy, but fails to survive in strains of B. subtilis 168.
mesh_terms: D001412:Bacillus subtilis / Q000187:drug effects; D004269:DNA, Bacterial; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism; D005111:Extrachromosomal Inheritance; D007930:Leucine / Q000378:metabolism; D010957:Plasmids; D013752:Tetracycline / Q000494:pharmacology; D014170:Transformation, Genetic
chemical_list: D004269:DNA, Bacterial; D013752:Tetracycline; D007930:Leucine