title: ELIEDA (enzyme-linked-immuno-electro-diffusion-assay): application of a combined immunoelectrodiffusion and immunoenzyme method to the study of immune response in parasitic infections.
abstract: The sensitivity of the technique of immunoelectrodiffusion (IED) has been enhanced by combining it with an enzyme method; this also enabled the class of immunoglobulins involved in the reactions to be determined by treating the precipitated immune complexes with enzyme-labelled class-specific antisera. The method has been employed in studies of the immunology of parasitic infections and these confirmed its sensitivity and specificity. ELIEDA provides a valuable tool for analysing responses to complex immunogens.
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D000936:Antigen-Antibody Complex; D004306:Dose-Response Relationship, Immunologic; D006041:Goats; D006801:Humans; D005779:Immunodiffusion; D007124:Immunoenzyme Techniques; D010272:Parasitic Diseases / Q000276:immunology*; D013997:Time Factors
chemical_list: D000936:Antigen-Antibody Complex