Sucrase and lactase synthesis in suckling rat intestine in response to substrate administration.
The intestinal brush border disaccharidases separated by gel electrophoresis were studied after oral administration of a high sucrose or lactose diet to 11-day-old suckling rats during 3 days. Some modifications of the brush border protein and eyzyme patterns could be attributed to the effect of the basic diet: increase of glucoamylase, appearance of a weak sucrase activity and of a second molecular form of maltase. However, the specific action of a given disaccharide on the synthesis of the corresponding hydrolytic enzyme could be clearly demonstrated. Indeed, the electrophoretic pattern after sucrose or lactose feeding showed a marked increase of the protein bands corresponding to sucrase-isomaltase or lactase activities.
D000284:Administration, Oral; D000818:Animals; D000831:Animals, Newborn; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005696:Galactosidases / Q000096:biosynthesis*; D007413:Intestinal Mucosa / Q000201:enzymology*; D007421:Intestine, Small / Q000201:enzymology; D007785:Lactose / Q000008:administration & dosage; D011247:Pregnancy; D051381:Rats; D013393:Sucrase / Q000096:biosynthesis*; D013395:Sucrose / Q000008:administration & dosage; D001616:beta-Galactosidase / Q000096:biosynthesis*
D013395:Sucrose; D005696:Galactosidases; D001616:beta-Galactosidase; D013393:Sucrase; D007785:Lactose