[Initiation of transcription and translation in E. coli nucleoids].
The initiation of both transcription and translation occurs in membrane-associated nucleoid of E. coli without the addition of cytoplasmic fraction. Initiation of translation, however, is stimulated by adjunction of S 165. Our results suggest that the system is limited in synthesizing F-met-tRNA. The capacity of nucleoid-extracted ribosomes to form initiation complexes is only slightly higher than that of supernatant ribosomes. RNA synthesis is partially rifampicin-sensitive, which implies that initiation of new chains takes place in our system.
D002462:Cell Membrane; D002467:Cell Nucleus / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D007700:Kinetics; D008715:Methionine; D009239:N-Formylmethionine; D014176:Protein Biosynthesis; D012343:RNA, Transfer / Q000378:metabolism; D012270:Ribosomes / Q000378:metabolism; D014158:Transcription, Genetic
D009239:N-Formylmethionine; D012343:RNA, Transfer; D008715:Methionine; D012321:DNA-Directed RNA Polymerases
Initiation de la transcription et de la traduction par le nucléoïde d'Escherichia coli.