The use of single-radial-haemolysis for rubella antibody studies.
The use of a single-radial-haemolysis technique for the detection of antibody to rubella virus is described. The single-radial-haemolysis test was compared with the standard HI methods for the detection of antibody to rubella virus. A close correlation between the two methods was observed in a survey of over two thousand serum samples and the study indicated that single-radial-haemolysis was highly satisfactory as an assay method for IgG antibodies to rubella virus. It was found that the immuno-globulins active in SRH tests sedimented in the 7S range in sucrose rate gradients and were presumably immunoglobulins of the IgG class, but 19S immunoglobulins did not produce haemolysis.
D000293:Adolescent; D000328:Adult; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis*; D010856:Columbidae / Q000097:blood; D003168:Complement Fixation Tests; D004912:Erythrocytes / Q000276:immunology; D000882:Haplorhini / Q000276:immunology; D006385:Hemagglutination Inhibition Tests; D006462:Hemolytic Plaque Technique; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis; D012409:Rubella / Q000276:immunology*
D000914:Antibodies, Viral; D007074:Immunoglobulin G