Influence of L-thyroxine upon enzymatic activity in the renal tubular epithelium of the rat under normal conditions and mercury-induced lesions. II. Histochemical studies of lactate dehydrogenase, succinate dehydrogenase, malate dehydrogenase, unspecific esterase, and glucose-6-phosphate dehydrogenase.
Mercury-induced renal tubular lesions in the rat present histochemically with a decrease of succinate dehydrogenase (SDH), malate dehydrogenase (MDH), glucose-6-phosphate dehydrogenase (G-6-PD), and unspecific esterase (UE), but with an increase of lactate dehydrogenase (LDH), indicating a drop of energy supply as well as a switch from oxidative to glycolytic energy production. L-thyroxine has the same effect on SDH, G-6-PD, and LDH, but an inverse effect on MDH and UE, pointing to stimulation of gluconeogenesis. However, administration of L-thyroxine to animals which have been submitted to sublimate intoxication even further decreases the MDH and UE activity while raising or partly restoring the activity of LDH, SDH, and G-6-PD. This observation is interpreted as an attempt of the damaged epithelial cell, as the gluconeogenesis ceases, to gain relatively more energy supply for the benefit of the vitally indispensable tubular Na+ reabsorption.
D058186:Acute Kidney Injury / Q000139:chemically induced / Q000201:enzymology*; D000818:Animals; D004847:Epithelial Cells; D004848:Epithelium / Q000201:enzymology; D004950:Esterases / Q000032:analysis*; D005954:Glucosephosphate Dehydrogenase / Q000032:analysis; D006651:Histocytochemistry; D007684:Kidney Tubules / Q000201:enzymology*; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008291:Malate Dehydrogenase / Q000032:analysis; D008297:Male; D008630:Mercury Poisoning / Q000201:enzymology*; D010088:Oxidoreductases / Q000032:analysis*; D051381:Rats; D013385:Succinate Dehydrogenase / Q000032:analysis; D013974:Thyroxine / Q000494:pharmacology*
D010088:Oxidoreductases; D007770:L-Lactate Dehydrogenase; D008291:Malate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase; D013385:Succinate Dehydrogenase; D004950:Esterases; D013974:Thyroxine