Biosynthesis of the yeast cell wall: selective assays and regulation of some mannosyl transferase activities.
Assays have been developed for some transfer reactions involved in the synthesis of Saccharomyces cerevisiae wall mannoproteins, both in a particulate preparation in the presence of EDTA or Triton X-100, and after lipid extraction with chloroform-methanol at -20 C. The mannosyl transferase activities were also studied in cells made permeable to GDP-mannose by toluene-ethanol treatment ("in situ"). In these permeabilized cells, the glycosylating reactions dependent on lipid carriers (dolichol derivatives) did not function, but those independent of them were unaffected. The lipid-independent mannosyl transferase activities were partially inhibited by nucleotide diphosphates probably in a competitive manner. Increase of the nucleotide diphosphate pool "in vivo" might slow down the speed of the transfer reactions carried out by the mannan synthetase system.
D000244:Adenosine Diphosphate / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D000431:Ethanol / Q000494:pharmacology; D006153:Guanosine Diphosphate / Q000494:pharmacology; D006155:Guanosine Diphosphate Mannose / Q000378:metabolism; D006602:Hexosyltransferases / Q000378:metabolism*; D008055:Lipids / Q000502:physiology; D008358:Mannose / Q000378:metabolism; D008364:Mannosyltransferases / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D014050:Toluene / Q000494:pharmacology; D014530:Uridine Diphosphate / Q000494:pharmacology
D008055:Lipids; D006153:Guanosine Diphosphate; D006155:Guanosine Diphosphate Mannose; D014050:Toluene; D000431:Ethanol; D011092:Polyethylene Glycols; D014530:Uridine Diphosphate; D000244:Adenosine Diphosphate; D004492:Edetic Acid; D006602:Hexosyltransferases; D008364:Mannosyltransferases; D008358:Mannose