title: Acetylated methylmannose polysaccharide of Streptomyces.
abstract: A polysaccharide composed of 3-O-methyl-D-mannose and D-mannose in a molar ratio of approximately 10:1 and containing 3 to 4 esterified acetyl residues has been isolated from Streptomyces griseus. This acetylated methylmannose polysaccharide (AMMP) is similar to the methylmannose polysaccharide (MMP) of Mycobacterium smegmatis (Gray, G. R., and Ballou, C. E. (1971) J. Biol. Chem. 246, 6835-6842) in its size and composition, the absence of acidic or basic groups, and the lack of a reducing end. It is different, however, in its content of esterified acetyl residues, and it is slightly different in its structure and in its gel filtration properties. The structure of AMMP has been established by proton magnetic resonance spectroscopy, and by combinations of methylation analysis and Smith degradation utilizing non-radioactively labeled polysaccharide and [3H]methyl-labeled polysaccharide obtained from cells grown in the presence of L-[methyl-3H]methionine. It is concluded that AMMP is a linear, nonreducing, neutral polysaccharide composed of a terminal D-mannose residue linked alpha(1 leads to 4) to a chain of 10 consecutive alpha(1 leads to 4)-linked 3-O-methyl-D-mannose residues. The reducing terminal 3-O-methyl-D-mannose residue exists, at least in part, as its alpha-methyl glycoside. The positions of attachment of the ester residues have not been established.
mesh_terms: D000107:Acetylation; D002849:Chromatography, Gas; D002850:Chromatography, Gel; D009682:Magnetic Resonance Spectroscopy; D008759:Methylglycosides / Q000032:analysis; D008766:Methylmannosides / Q000032:analysis; D008968:Molecular Conformation; D009161:Mycobacterium; D009904:Optical Rotation; D011135:Polysaccharides, Bacterial / Q000302:isolation & purification; D013305:Streptomyces griseus / Q000032:analysis*
chemical_list: D008759:Methylglycosides; D008766:Methylmannosides; D011135:Polysaccharides, Bacterial