Conversion by trypsin of nonsense suppressor-produced isozymes of triosephosphate isomerase to isozymes resembling the wild type.
Nonsense suppressor genes caused the synthesis of new triosephosphate isomerase isozymes in Bacillus subtilis. Incubation with trypsin produced a large decrease in the apparent molecular weight of one such isozyme and simultaneously changed the electrophoretic behavior such that it resembled that of the wild-type enzyme.
D001412:Bacillus subtilis / Q000187:drug effects / Q000201:enzymology*; D002238:Carbohydrate Epimerases / Q000378:metabolism*; D004586:Electrophoresis; D007527:Isoenzymes / Q000378:metabolism*; D008970:Molecular Weight; D013489:Suppression, Genetic; D014305:Triose-Phosphate Isomerase / Q000378:metabolism*; D014357:Trypsin / Q000494:pharmacology*
D007527:Isoenzymes; D014357:Trypsin; D002238:Carbohydrate Epimerases; D014305:Triose-Phosphate Isomerase