Automated differential cell counting of viable cells.
A rapid, automated method of differentially counting viable cells by ethidium bromide-acridine orange cytofluorography is described (Takasugi 1971). The method is shown to have comparability, equal precision, and sensitivity to the standard trypan blue, visual method. The method is applicable to microcytotoxicity testing.
D001331:Automation; D002452:Cell Count / Q000295:instrumentation / Q000379:methods*; D005470:Fluorometry / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D007958:Leukocyte Count / Q000295:instrumentation / Q000379:methods; D007962:Leukocytes / Q000166:cytology; D008214:Lymphocytes / Q000166:cytology