Platelet immuno-assay (PIA) as a simple and sensitive technique for the detection of antigens.
The platelet immuno-assay (PIA) is a simple and inexpensive technique to detect antigens with ng/ml sensitivity using micro-titer equipment and commercially available antisera. It can be easily adapted for virtually any antigen to which rabbit antiserum containing specific IgG-antibodies cna be obtained. The assay is inhibited by IgG and C1q in amounts greater than or equal to 125 microgram/ml and 12.5 microgram/ml respectively. Its application is thus limited to those fluids where IgG and C1q are present in less than these concentrations.
D000818:Animals; D000941:Antigens / Q000032:analysis*; D001792:Blood Platelets / Q000276:immunology*; D003172:Complement C1; D004306:Dose-Response Relationship, Immunologic; D006801:Humans; D007118:Immunoassay; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D010974:Platelet Aggregation; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000276:immunology; D001616:beta-Galactosidase / Q000276:immunology
D000941:Antigens; D003172:Complement C1; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D012710:Serum Albumin, Bovine; D001616:beta-Galactosidase