Increased histaminase activity in the atrophic muscle of denervated frog.
Sciatic denervation for 1 month in the frog Rana hexadactyla resulted in progressive atrophy of the gastrocnemius muscle without any change in the total DNA content of the whole muscle. Histamine content of the muscle decreased; glutamic and acid content increased and histidine level remained unaltered on denervation. Histaminase activity localized in the muscle decreased; glutamic acid content increased and histidine level remained unaltered on denervation. Histaminase activity localized in the muscle mitochondria increased on denervation. The histidine-degrading enzymes, histidine ammonia lyase, urocanate hydratase and imidazol-5yl lactate dehydrogenase, are localized in the sarcoplasm of the muscle and their activities are not altered on denervation. The histidine decarboxylase activity localized in the mitochondria is not altered on denervation. The reduction in the histamine content of the atrophied muscle may be due to increased mitochondrial histaminase activity but not due to increased decarboxylation of histidine. The loss of 'trophic influence' due to denervation may be manifested in the impairment of mitochondrial histaminase activity.
D000109:Acetylcholine / Q000378:metabolism; D006631:Amine Oxidase (Copper-Containing) / Q000378:metabolism*; D000818:Animals; D001001:Anura; D001284:Atrophy / Q000201:enzymology; D005971:Glutamates / Q000378:metabolism; D006632:Histamine / Q000378:metabolism; D006639:Histidine / Q000378:metabolism; D009121:Muscle Denervation; D009132:Muscles / Q000201:enzymology* / Q000473:pathology; D011898:Ranidae; D013347:Subcellular Fractions / Q000201:enzymology
D005971:Glutamates; D006639:Histidine; D006632:Histamine; D006631:Amine Oxidase (Copper-Containing); D000109:Acetylcholine