title: In vitro growth of imaginal disks from Drosophila melanogaster.
abstract: Imaginal disks were cultured in a new medium in vitrol Optimal growth occurred when the medium was supplemented with insulin and a juvenile hormone analog and conditioned by larval fat body. The disks grow in vitro by normal cell division and maintain their capacity to differentiate into normal patterns of adult cuticular structures.
mesh_terms: D000273:Adipose Tissue / Q000502:physiology; D000818:Animals; D002454:Cell Differentiation; D002455:Cell Division; D003470:Culture Media; D046508:Culture Techniques; D004331:Drosophila melanogaster / Q000033:anatomy & histology / Q000254:growth & development*; D005121:Extremities / Q000166:cytology / Q000254:growth & development; D007328:Insulin / Q000494:pharmacology; D007814:Larva / Q000254:growth & development; D008675:Metamorphosis, Biological; D014921:Wings, Animal / Q000166:cytology / Q000254:growth & development
chemical_list: D003470:Culture Media; D007328:Insulin