title: Comparison of four culture media for isolation of Mycobacterium avium complex from porcine tissues.
abstract: The efficiency of four culture media was compared for the isolation of Mycobacterium avium complex from 197 procine tissues. In 82 tissues with microscopic granulomas and acid-fast bacilli, a significantly greater number of isolates were obtained on Middlebrook 7H10 medium with sodium pyruvate than on Stonebrink medium, Herrold egg yolk agar medium, or Lowenstein-Jensen medium (P=0.01). In 46 tissues in which no microscopic granulomas or acid-fast bacilli were observed, a significantly greater number of isolates were made on Middlebrook 7H10 medium or Herrold egg yolk agar medium than on Stonebrink medium or on Lowenstein-Jensen medium (P=0.01). The time required to grow M. avium complex on Lowenstein-Jensen medium was significantly greater than the time required to observe growth on Stonebrink, Middlebrook 7H10, or Herrold egg yolk agar medium (p=0.001).
mesh_terms: D000818:Animals; D003470:Culture Media; D008198:Lymph Nodes / Q000382:microbiology; D009161:Mycobacterium / Q000302:isolation & purification*; D009162:Mycobacterium avium / Q000145:classification / Q000302:isolation & purification*; D012703:Serotyping; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*; D014376:Tuberculosis / Q000382:microbiology / Q000662:veterinary*
chemical_list: D003470:Culture Media