[Comparison in genetically obese and normal rats in the secretion of lipids synthesized from labeled lauric and oleic acids and glycerol in isolated perfused liver].
(1) Isolated livers of previously fasted fafa and Wistar Rats are perfused with labelled oleic acid and glycerol. Two pools of synthesized lipids are studied: --a pool of liver lipids: labelled TG 16:0, 16:0, 18:1, 16:0, 18:1, 18:1 and 18:1, 18:1, 18:1 are synthesized more by the fafa than by the Wistar Rat liver, The TG 16:0, 18:1, 18:2 appears in the liver of the 2 rat strains. After a 30 min. perfusion, the PL are synthesized less by the fafa than by Wistar Rat liver; --a pool of lipids released in the circulation. The labelled tG 16:0, 18:1, 18:2, are increased in the perfusate of fafa rat. The labelled TG 18:1, 18:1, 18:2 and 18:1, 18:2, 18:2 are in the same amount. (2) The liver synthesis of TG 12:0, 12:0, 12:0 is demonstrated in the fafa Rat by a lauric acid perfusion. This TG is not present in circulation, when a Wistar Rat liver synthesizes and releases with this TG, TG containing 1 or 2 molecules of lauric acid.
D000818:Animals; D002250:Carbon Radioisotopes; D005990:Glycerol / Q000378:metabolism*; D006720:Homozygote; D066298:In Vitro Techniques; D007553:Isotope Labeling; D007850:Lauric Acids / Q000378:metabolism*; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D009765:Obesity / Q000235:genetics / Q000378:metabolism*; D009829:Oleic Acids / Q000378:metabolism*; D010743:Phospholipids / Q000096:biosynthesis; D051381:Rats; D014280:Triglycerides / Q000096:biosynthesis; D014316:Tritium
D002250:Carbon Radioisotopes; D007850:Lauric Acids; D009829:Oleic Acids; D010743:Phospholipids; D014280:Triglycerides; D014316:Tritium; D005990:Glycerol
Comparaison chez des rats génétiquement obèses et des Rats normaux des sécrétions des lipides synthétisés par les foies isolés et perfusés par de l'acide laurique, de l'acide oléique et du glycérol marqués.