Genetic loci of hemolysin production in Streptococcus faecalis subsp. zymogenes.
Two plasmids corresponding to molecular weights of 38.5 X 10(6) and 3.6 X 10(6) have been identified in Streptococcus faecalis subsp. zymogenes strain X-14. The larger plasmid is required for hemolysin-bacteriolysin production. Strain L2, a nonlytic nitrosoquanidine mutant of strain X-14, still harbors the hemolytic plasmid and produces the lysin component, but not the activator component, of the lytic system. Conjugal transfer of this plasmid from strain L2 to plasmid-free strains and strains cured of the 38.5-megadalton plasmid gives rise to hemolytic recipients. This implicates a gene in hemolysin production at a site other than the 38.5-megadalton plasmid.
D001431:Bacteriological Techniques; D001433:Bacteriolysis; D002499:Centrifugation, Density Gradient; D002874:Chromosome Mapping; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000302:isolation & purification; D013293:Enterococcus faecalis / Q000032:analysis / Q000378:metabolism*; D006460:Hemolysin Proteins / Q000096:biosynthesis*; D010641:Phenotype; D010957:Plasmids; D013010:Sonication
D004269:DNA, Bacterial; D006460:Hemolysin Proteins