Further studies on the enzymatic conversion of prostaglandin endoperoxide into prostacyclin by porcine aorta microsomes.
A simple, rapid radiochemical assay for prostacyclin synthesis has been used to characterize the enzyme in arterial walls which converts prostaglandin endoperoxides to prostacyclin. The enzyme displays a broad pH optimum, and catalyses a rapid conversion of saturating concentrations of the endoperoxide at 37 degrees C. Hydroperoxides of several unsaturated fatty acids are potent inhibitors of the enzyme, and act in a time dependent manner. The isomerase which converts prostaglandin endoperoxides to prostaglandin E2 or D2 was not detected in the arterial wall.
D000818:Animals; D001011:Aorta / Q000201:enzymology*; D011464:Epoprostenol / Q000096:biosynthesis*; D007700:Kinetics; D008861:Microsomes / Q000201:enzymology*; D011449:Prostaglandin Endoperoxides / Q000378:metabolism*; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism*; D011453:Prostaglandins / Q000096:biosynthesis*; D013552:Swine
D011449:Prostaglandin Endoperoxides; D011453:Prostaglandins; D011464:Epoprostenol; D011451:Prostaglandin-Endoperoxide Synthases