Further studies on a vaccinia virus cytotoxin present in infected cell extracts: identification as surface tubule monomer and possible mode of action.
A vaccinia virus-specific cytotoxic protein (3--10 X 10(4) daltons) had previously been detected in extracts of infected HeLa cells. In this study the protein has been shown to be a late gene product and a virion component--almost certainly the monomer of the surface tubules of the vaccinia virion. The relationship of this cytotoxin to a number of early vaccinia-induced cytopathic effects was examined: it was not the mediator of vaccinia-induced early cell rounding, did not inhibit protein or RNA synthesis in cell-free systems or intact cells (after uptake-induction by hypertonic MgSO4), or cause the release of beta-glucuronidase from a crude preparation of HeLa cell lysosomes. Its possible role in vaccinia-induced cell degeneration, late in infection, is discussed.
D002474:Cell-Free System; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D003603:Cytotoxins / Q000032:analysis* / Q000494:pharmacology; D004273:DNA, Neoplasm / Q000096:biosynthesis; D006367:HeLa Cells; D008247:Lysosomes / Q000187:drug effects; D009363:Neoplasm Proteins / Q000096:biosynthesis; D012334:RNA, Neoplasm / Q000096:biosynthesis; D014616:Vaccinia virus / Q000032:analysis*; D014764:Viral Proteins / Q000032:analysis* / Q000494:pharmacology
D003603:Cytotoxins; D004273:DNA, Neoplasm; D009363:Neoplasm Proteins; D012334:RNA, Neoplasm; D014764:Viral Proteins