RNA sequences in ribonucleoprotein fragments of the complex formed from ribosomal 23-S RNA and ribosomal protein L24 of Escherichia coli.
Upon digestion of the complex formed from the 23-S ribosomal RNA and the 50-S ribosomal protein L24 of Escherichia coli, two fragments resistant to ribonuclease were recovered; these fragments contained RNA sections belonging to the 480 nucleotides at the 5' end of 23-S RNA. By determining the sequence of 70% of this latter region we were able to localise the sections which, in the presence of the protein, are resistant to ribonuclease. Our results suggest that the region encompassing the 480 nucleotides starting at the 9th nucleotide from the 5' end of 23-S RNA has a compact tertiary structure, which is stabilised by protein L24.
D000469:Alkaline Phosphatase; D001483:Base Sequence; D004926:Escherichia coli / Q000032:analysis*; D008274:Magnesium; D008970:Molecular Weight; D009698:Nucleoproteins; D009843:Oligoribonucleotides / Q000032:analysis; D010179:Pancreas / Q000201:enzymology; D012335:RNA, Ribosomal; D006163:Ribonuclease T1; D012260:Ribonucleases; D012261:Ribonucleoproteins; D012269:Ribosomal Proteins
D009698:Nucleoproteins; D009843:Oligoribonucleotides; D012335:RNA, Ribosomal; D012261:Ribonucleoproteins; D012269:Ribosomal Proteins; D012260:Ribonucleases; D006163:Ribonuclease T1; D000469:Alkaline Phosphatase; D008274:Magnesium