Purine degradation in Pseudomonas aeruginosa and Pseudomonas testosteroni.
1. Adenine, hypoxanthine, xanthine and guanine are broken down in Pseudomonas aeruginosa and Pseudomonas testosteroni to allantoin by the concerted action of the enzymes adenine deaminase, guanine deaminase, NAD+-dependent xanthine dehydrogenase and uricase. 2. Uric acid is broken down by an unstable, membrane-bound uricase with an unusually low pH optimum. 3. In both strains adenine inhibits growth and xanthine dehydrogenase. A second type of inhibition is manifest only in Ps. testosteroni and concerns the regulation of the biosynthesis of amino acids of the aspartate family. Enzymic studies showed that in this strain aspartate kinase is inhibited by AMP.
D000225:Adenine / Q000494:pharmacology; D000249:Adenosine Monophosphate / Q000494:pharmacology; D000481:Allantoin / Q000096:biosynthesis; D000619:Aminohydrolases / Q000378:metabolism; D001222:Aspartate Kinase / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D006147:Guanine; D011549:Pseudomonas / Q000187:drug effects / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D011687:Purines / Q000378:metabolism*; D013045:Species Specificity; D014503:Urate Oxidase / Q000378:metabolism; D014968:Xanthine Dehydrogenase / Q000378:metabolism
D011687:Purines; D000481:Allantoin; D000249:Adenosine Monophosphate; D006147:Guanine; D014968:Xanthine Dehydrogenase; D014503:Urate Oxidase; D001222:Aspartate Kinase; D000619:Aminohydrolases; D000225:Adenine