A general method for the lysis of Streptomyces species.
A procedure for the gentle lysis of Streptomyces is described. Complete lysis is obtained and covalently closed circular (CCC) DNA remains intact. This procedure was successfully used to lyse 14 different species of Streptomyces.
D001431:Bacteriological Techniques; D001433:Bacteriolysis; D004269:DNA, Bacterial / Q000302:isolation & purification; D004270:DNA, Circular / Q000302:isolation & purification; D004492:Edetic Acid / Q000494:pharmacology; D009113:Muramidase / Q000494:pharmacology; D010957:Plasmids; D013045:Species Specificity; D013302:Streptomyces / Q000032:analysis
D004269:DNA, Bacterial; D004270:DNA, Circular; D004492:Edetic Acid; D009113:Muramidase