title: Reduction of beta-galactosidase in the ketotic Chinese hamster kidney.
abstract: Kidneys from normal, diabetic-nonketotic and ketotic Chinese hamsters were homogenized, fractionated and assayed for beta-glucosidase, and beta-galactosidase activities. The kidneys of the ketotic animals were enlarged but the protein content in each subcellular fraction was similar in all three groups of animals. beta-Glucosidase was found chiefly in the soluble fraction and no difference was observed in these animals. beta-Galactosidase was distributed in both cytoplasmic and particulate fractions; difference in the specific activity of beta-galactosidase between control and ketotic animals was found in nuclear, lysosomal-mitochondrial, microsomal and soluble fractions.
mesh_terms: D000138:Acidosis / Q000201:enzymology*; D000818:Animals; D006224:Cricetinae; D003412:Cricetulus; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology; D005696:Galactosidases / Q000378:metabolism*; D007662:Ketosis / Q000201:enzymology*; D007668:Kidney / Q000201:enzymology* / Q000378:metabolism; D001616:beta-Galactosidase / Q000378:metabolism*; D001617:beta-Glucosidase / Q000378:metabolism
chemical_list: D005696:Galactosidases; D001617:beta-Glucosidase; D001616:beta-Galactosidase