[Use of acridine yperite in the selection of Act. nodosus, a producer of amphotericin B].
The effect of acridine-yperite (AY) on Act. nodosus LIA 0861 at various physiological stages of development was studied. It was shown that both the spores and the germs were sensitive to AY. The level of the mutagen effect depended on the exposure time and the physiological state of the Act. nodosus cells during the treatment. The treatment of the spore suspension with AY had an insignificant effect on the strain morphological variation, while the number of the morphologically changed colonies markedly decreased when 5-hour germs were subjected to the mutagen effect. A decrease in the average antibiotic activity of Act. nodosus was observed, when its spores were subjected to the treatment with AY, which was associated with a decrease in the number of the plus variants. The treatment of 5-hour germs increased the average antibiotic activity of the culture which correlated with an increase in the number of the plus variants.
D000166:Acridines / Q000494:pharmacology*; D000666:Amphotericin B / Q000096:biosynthesis*; D014644:Genetic Variation / Q000187:drug effects; D009150:Mustard Compounds / Q000494:pharmacology*; D009151:Mustard Gas / Q000494:pharmacology*; D009153:Mutagens; D012641:Selection, Genetic; D013171:Spores, Bacterial / Q000187:drug effects; D013302:Streptomyces / Q000187:drug effects* / Q000378:metabolism; D013997:Time Factors
D000166:Acridines; D009150:Mustard Compounds; D009153:Mutagens; C018806:acridine yperite; D000666:Amphotericin B; D009151:Mustard Gas
Ispol'zovanie akridin-iprita v selektsii Act. nodosus--produtsenta amfoteritsina B.