Purine nucleoside phosphorylase polymorphism in sheep erythrocytes.
A polymorphism of purine nucleoside phosphorylase is described in sheep erythrocytes. Two isozymes were distinguished electrophoretically, one with high activity (NP-1) and one with low activity (NP-2). Breeding data suggest that the two isozymes are the product of two codominant alleles, NP1 and NP2. The Km's for inosine did not differ between NP-1 and NP-2; however, NP-2 had a lower pH optimum and was relatively unstable when incubated at 48 C.
D000483:Alleles; D000818:Animals; D004912:Erythrocytes / Q000201:enzymology*; D005260:Female; D007527:Isoenzymes / Q000097:blood*; D010430:Pentosyltransferases / Q000097:blood*; D011110:Polymorphism, Genetic; D011683:Purine-Nucleoside Phosphorylase / Q000097:blood*; D012756:Sheep / Q000097:blood*
D007527:Isoenzymes; D010430:Pentosyltransferases; D011683:Purine-Nucleoside Phosphorylase