A sulphated glycopeptide in human supragingival calculus extracts.
A method is described for the isolation and purification of a sulphated glycopeptide from human supragingival calculus. The compound was isolated after using EDTA treatment, 2 M CaCl2 extraction, proteolytic digestion, ethanol precipitation, and finally purified by DEAE cellulose chromatography. It migrated as a single component of cellulose acetate electrophoresis, and chemical and infrared spectral analysis showed the presence of covalently attached sulphate groups. The sulphated glycopeptide was distinguished from being a sulphated glycosaminoglycan.
D002122:Calcium Chloride; D002848:Chromatography, DEAE-Cellulose; D003728:Dental Calculus / Q000032:analysis*; D004492:Edetic Acid; D004586:Electrophoresis; D006020:Glycopeptides / Q000302:isolation & purification*; D006801:Humans; D008722:Methods; D013055:Spectrophotometry, Infrared; D013431:Sulfates
D006020:Glycopeptides; D013431:Sulfates; D004492:Edetic Acid; D002122:Calcium Chloride