Metabolism of uracil-containing DNA: degradation of bacteriophage PBS2 DNA in Bacillus subtilis.
When Bacillus subtilis is infected by the uracil-containing DNA phage PBS2, the parental DNA labeled with radioactive uracil and cytosine remains acid insoluble. If the synthesis of the phage-induced uracil-DNA N-glycosidase inhibitor is prevented, the parental DNA is completely degraded to acid-soluble products beginning at about 6 min after infection. The host N-glycosidase probably initiates the degradation pathway, with nucleases being responsible for the remaining degradation of the DNA.
D001412:Bacillus subtilis / Q000201:enzymology / Q000378:metabolism*; D001435:Bacteriophages / Q000378:metabolism*; D002701:Chloramphenicol / Q000494:pharmacology; D003596:Cytosine / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism*; D003609:Dactinomycin / Q000494:pharmacology; D006026:Glycoside Hydrolases / Q000378:metabolism; D009691:Nucleic Acid Denaturation; D012995:Solubility; D014498:Uracil / Q000378:metabolism
D004279:DNA, Viral; D003609:Dactinomycin; D014498:Uracil; D002701:Chloramphenicol; D003596:Cytosine; D006026:Glycoside Hydrolases