title: 5.9-S RNA, a new RNA characterized in several mammalian cell lines.
abstract: A new species of RNA has been isolated from several different cell lines, both oncornavirus producing and non-producing. This RNA, which we designate 5.9-S RNA is present in the cellular cytoplasmic fraction at very low concentration (approximately 1% of the quantity of 4-S RNA), but it accumulates to much higher levels in two murine oncornaviruses, Moloney murine sarcoma leukemia virus complex and Gross leukemia virus, where it represents as much as 10% of the low-molecular-weight RNA fraction associated with the 70-S RNA genome. The electrophoretic mobility and fingerprint analysis of T1 RNase digest products show that this species of RNA is approximately 160-165-residues long, and can be unequivocally distinguished from all previously described species of RNA in this size range.
mesh_terms: D000406:AKR murine leukemia virus; D001483:Base Sequence; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D008970:Molecular Weight; D008979:Moloney murine leukemia virus; D009691:Nucleic Acid Denaturation; D009843:Oligoribonucleotides / Q000032:analysis; D010179:Pancreas / Q000201:enzymology; D012313:RNA / Q000302:isolation & purification; D012334:RNA, Neoplasm / Q000302:isolation & purification; D012367:RNA, Viral / Q000302:isolation & purification; D006163:Ribonuclease T1; D012260:Ribonucleases
chemical_list: D009843:Oligoribonucleotides; D012334:RNA, Neoplasm; D012367:RNA, Viral; D012313:RNA; D012260:Ribonucleases; D006163:Ribonuclease T1