Isolation and molecular size of Clostridium botulinum type C toxin.
A procedure is described for the purification of hemagglutinin-free Clostridium botulinum type C toxin. The toxin was purified approximately 1,000-fold from the original culture supernatant in an overall yield of 60% to a final specific toxicity of 4.4 x 10(7) minimal lethal doses/mg of protein. The toxin had a molecular weight of 141,000 and consisted of a heavy and a light chain. The molecular weights of the subunits were approximately 98,000 and 53,000. When comparing the molecular size and composition of type C toxin to that of botulinum toxins of different types, some common features may be suggested; i.e., the toxin has a molecular weight between 141,000 to 160,000 and is comprised of a heavy and a light chain linked by disulfide bonds (or bond).
D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis; D001905:Botulinum Toxins / Q000032:analysis / Q000302:isolation & purification / Q000633:toxicity; D002845:Chromatography; D002850:Chromatography, Gel; D005779:Immunodiffusion; D051379:Mice; D008970:Molecular Weight
D001426:Bacterial Proteins; D001905:Botulinum Toxins