Detection of IgG antibodies to type-specific Pseudomonas aeruginosa  lipopolysaccharides by solid-phase radioimmunoassay.
Previous studies have suggested that IgG serotype-specific antibodies are protective against infections with pseudomonas aeruginosa. In the present study, type-specific IgG antibodies to P. aeruginosa were detected by solid-phase radioimmunoassay in sera from 15 volunteers before and after vaccination with lipopolysaccharides from P. aeruginosa and from four patients with endocarditis due to P. aeruginosa. Significant type-specific increases in IgG antibody occurred after both vaccination and infection. The correlation coefficients comparing net counts per minute by solid-phase radioimmunoassay with hemagglutination titers in the 15 vaccinees were 0.940, 0.874, 0.792, 0.903, 0.882, 0.869, and 0.704 for serotypes 1--7, respectively.
D000328:Adult; D000918:Antibody Specificity; D004697:Endocarditis, Bacterial / Q000276:immunology; D006386:Hemagglutination Tests; D006801:Humans; D007074:Immunoglobulin G; D008070:Lipopolysaccharides / Q000276:immunology; D008297:Male; D011135:Polysaccharides, Bacterial; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D011863:Radioimmunoassay
D007074:Immunoglobulin G; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial