The use of fungal protoplasts in the study of aflatoxin biosynthesis.
Protoplasts derived from Aspergillus flavus are shown to be capable of synthesizing aflatoxins when incubated in a chemically defined medium. 14C-Acetate and 14C-Versicolorin A, added to protoplasts from 3-day-old mycelium, are incorporated into aflatoxin B1.
D000085:Acetates / Q000378:metabolism; D000348:Aflatoxins / Q000096:biosynthesis*; D000880:Anthraquinones / Q000378:metabolism; D001231:Aspergillus flavus / Q000378:metabolism*; D011523:Protoplasts / Q000378:metabolism*
D000085:Acetates; D000348:Aflatoxins; D000880:Anthraquinones