Comparison of vascular smooth muscle cells from adult human, monkey and rabbit in primary culture and in subculture.
A method is presented for growing large numbers of pure isolated smooth muscle cells from adult human, monkey, and rabbit blood vessels in primary culture. In the first few days in culture these cells closely resembled those in vivo and could be induced to contract with angiotensin II, noradrenaline and mechanical stimulation. They stained intensely with antibodies against smooth muscle actin and myosin. Fibroblasts and endothelial cells did not stain with these antibodies thereby allowing the purity of each batch of cultures to be monitored. This was consistently found to be better than 99%. The smooth muscle cells modified or "dedifferentiated" after about 9 days in culture to morphologically resemble fibroblasts. At this stage cells could no longer be induced to contract and did not stain with the myosin antibodies. Intense proliferation of these cells soon resulted in a confluent monolayer being formed at which stage some differentiated characteristics returned. The modification of "dedifferentiation" process could be inhibited by the presence of a feeder layer of fibroblasts or endothelial cells, or the addition of cAMP to the culture medium. Smooth muscle cells which had migrated from explants in primary culture, and cells in subculture, had morphological and functional properties of "dedifferentiated" cells at all times. The advantages of differentiated rather than "dedifferentiated" smooth muscle cells in culture for the study of mitogenic agents in atherosclerosis is discussed.
D000199:Actins / Q000032:analysis; D000818:Animals; D001808:Blood Vessels; D002454:Cell Differentiation; D002455:Cell Division; D002465:Cell Movement; D002478:Cells, Cultured; D004727:Endothelium / Q000166:cytology; D005347:Fibroblasts; D005455:Fluorescent Antibody Technique; D000882:Haplorhini; D006801:Humans; D066298:In Vitro Techniques; D008253:Macaca mulatta; D008297:Male; D008875:Middle Aged; D009119:Muscle Contraction; D009130:Muscle, Smooth / Q000032:analysis / Q000166:cytology*; D009218:Myosins / Q000032:analysis; D011817:Rabbits; D013997:Time Factors
D000199:Actins; D009218:Myosins