Separation of two Salmonella O-antigen polysaccharides by means of a glutaraldehyde-concanavalin-A polymer. Primary studies on the separated fractions.
The Salmonella zuerich (1,9,12,(46),27) cell wall O-polysaccharides have been characterized as a mixture of two immunologically distinct fractions differing in the presence or absence of the antigenic determinant 1 usually carried by side chains of an alpha-D-glucosyl residue. In this paper, we describe the use of concanavalin A in separating the two O-polysaccharides. The procedure involves polymerization of concanavalin A with glutaraldehyde, followed by selective adsorption of the polysaccharide containing O factor 1 and elution with D-glucose. Immunological and chemical analyses showed that the separation was successful and demonstrated chemical differences between these two fractions in relation to their immunological behaviour.
D000818:Animals; D002473:Cell Wall / Q000032:analysis; D002846:Chromatography, Affinity; D003208:Concanavalin A; D005976:Glutaral; D007118:Immunoassay; D037102:Lectins; D011135:Polysaccharides, Bacterial / Q000302:isolation & purification; D011233:Precipitin Tests; D011817:Rabbits / Q000276:immunology; D012475:Salmonella / Q000032:analysis*
D037102:Lectins; D011135:Polysaccharides, Bacterial; D003208:Concanavalin A; D005976:Glutaral