[Use of labeling with 3H6-thymidine in the study of the multiplication of lactobacillus casei immobilized in a polyacrylamide gel].
When 3H6-thymidine labelled bacteria (1.2 desintegration per cell/day) are immobilized in a polyacrylamide gel lattice, the decimal reduction time for their malic acid decarboxylase activity is extended to 38 days. Thus, the growth of bacteria in the gel lattice is very poor. The author has evaluated both the DNA turnover rate during the exponential growth phase for the free cells and the DNA specific activity of the population of immobilized cells. The viability of bacteria isolated from the gel lattice decreases with time, as does their thermal stability for malic acid decarboxylase activity. This activity is partially restored after 65 days. With respect to the protein, deoxyribose and ribose cell constituents, the protein/deoxyribose, protein/ribose and deoxyribose/ribose ratios fluctuats considerably with time. These fluctuations are more marked than those observed for the free cells during their different growth phases.
D000178:Acrylamides / Q000494:pharmacology; D001426:Bacterial Proteins / Q000378:metabolism; D003470:Culture Media; D004269:DNA, Bacterial / Q000378:metabolism; D007780:Lacticaseibacillus casei / Q000254:growth & development* / Q000378:metabolism; D012329:RNA, Bacterial / Q000378:metabolism; D013936:Thymidine; D014316:Tritium
D000178:Acrylamides; D001426:Bacterial Proteins; D003470:Culture Media; D004269:DNA, Bacterial; D012329:RNA, Bacterial; D014316:Tritium; D013936:Thymidine
Application du marquage par la 3H6-thymidine à l'étude de la multiplication de Lactobacillus casei inclus dans un gel de polyacrylamide.