[Cytotoxic effect of the serum of amyotrophic lateral sclerosis patients on spinal cord motoneurons in tissue culture].
Organotypical cultures of the spinal cord of the fetal mouse were used to study the neurocytotoxic effect of sera from patients with amyotrophic lateral sclerosis (ALS). Sera from 16 patients with ALS were used in two experiments: 1. short-term treatment (up to 6 days) of the mature cultures with nutrient medium containing 30% of ALS serum; 2. long-term cultivation from the explantation up to 4 weeks of spinal cord in the medium containing 5-25% of ALS serum. No specific neurotoxic effect of ALS sera on the motor neurons of the cultured spinal cord were observed in acute or chronic experiments.
D000328:Adult; D000368:Aged; D000690:Amyotrophic Lateral Sclerosis / Q000097:blood*; D000818:Animals; D046508:Culture Techniques; D003602:Cytotoxicity, Immunologic; D006801:Humans; D007106:Immune Sera / Q000494:pharmacology*; D051379:Mice; D008875:Middle Aged; D009046:Motor Neurons; D009928:Organ Specificity; D013116:Spinal Cord
D007106:Immune Sera
O tsitotoksicheskom vliianii syvorotki krovi bol'nykh bokovym amiotroficheskim sklerozom na motoneĭrony spinnogo mozga v kul'ture tkani.