[Studies on the activation of humoral defense factors against bacteria in larvae of the wax moth Galleria mellonella L].
The change of lysozyme activity in the hemolymph of the wax moth larvae caused by vaccination is influenced by the size and the dose of the injected particles. The rate of incorporation of those particles into phagocytes has no effect on this process. The change of lysozyme activity correlats negatively with the amount of granulocytes after vaccination. There were no correlations with the amount of other types of the hemocytes, neither with the resistance of the larvae against Pseudomonas aeruginosa nor with the survival of gram-negative bacteria within the hemocoelom. Origin, regulation and role of lysozyme are discussed regarding the defence against gram-negative bacteria. In the hemolymph of wax moth larvae after vaccination the formation of spheroblasts has been observed with all gram-negative strains of bacteria studied. The speed and extent of the formation of spheroblasts corresponded to the pathogenicity of these bacteria for the larvae as well as to the survival of the bacteria within the hemocoelom.
D000907:Antibodies, Bacterial; D004926:Escherichia coli / Q000276:immunology; D006801:Humans; D007814:Larva / Q000276:immunology / Q000382:microbiology; D007915:Lepidoptera / Q000276:immunology*; D009036:Moths / Q000196:embryology / Q000276:immunology*; D010587:Phagocytosis; D011513:Proteus mirabilis / Q000276:immunology; D011550:Pseudomonas aeruginosa / Q000276:immunology
D000907:Antibodies, Bacterial
Untersuchungen über die Aktivierung humoraler Abwehrfaktoren gegen Bakterien bei Larven der Wachsmotte Galleria mellonella L.