title: The extraction and partial characterization of proteins released by decalcification from calcified human aortic plaques.
abstract: Tissue from severely atherosclerotic human aortas was divided into heavily calcified (plaque) and less calcified (non-plaque) areas. After defatting and thorough extraction with neutral salt solutions, further proteinaceous material was released from plaque than non-plaque areas. Although organic phosphorous was associated with the EDTA-solubilized protein from both plaque and non-plaque areas, the phosphorous to protein ratio of extracts from plaque areas was 2 to 3 times that from non-plaque areas. Analysis of the major protein component of the extract of plaque areas indicated an acidic glyco-protein which appeared to be associated with an organic phosphorous moiety as well as cholesterol and cholesterol esters.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D001011:Aorta / Q000032:analysis / Q000378:metabolism*; D001161:Arteriosclerosis / Q000378:metabolism*; D002114:Calcinosis / Q000378:metabolism*; D002784:Cholesterol / Q000032:analysis; D002788:Cholesterol Esters / Q000032:analysis; D004492:Edetic Acid; D006023:Glycoproteins / Q000032:analysis; D006801:Humans; D008903:Minerals / Q000032:analysis; D009943:Organophosphorus Compounds / Q000032:analysis; D010743:Phospholipids / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D012995:Solubility
chemical_list: D000596:Amino Acids; D002788:Cholesterol Esters; D006023:Glycoproteins; D008903:Minerals; D009943:Organophosphorus Compounds; D010743:Phospholipids; D011506:Proteins; D002784:Cholesterol; D004492:Edetic Acid