[Characterization of the "4 S-trypsin" form of the cytosoluble estradiol receptor of calf uterus in a nondenaturing system].
The characterisation of the "4 S-trypsin" form of the estradiol-receptor from calf uterus cytosol was carried out by polyacrylamide gel electrophoresis under non-denaturing conditions. In a multiphasic buffer system (upper buffer Tris-glycine pH25degrees C = 8,6, lower buffer Tris HCl pH25degrees C = 7,4), three radioactive estradiol peaks were observed. The first was free estradiol, the second estradiol initially complexed with a macromolecule and dissociated during electrophoresis, and the third the hormone-receptor complex. The sedimentation coefficient (4 S) of this complex was the same before and after electrophoresis. Its mean geometric radius is R = 2.53 + 0.08 nm and its molecular weight was estimated to be 55,000.
D000818:Animals; D002417:Cattle; D003600:Cytosol / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D046911:Macromolecular Substances; D008970:Molecular Weight; D011960:Receptors, Estrogen / Q000302:isolation & purification / Q000378:metabolism; D014357:Trypsin; D014599:Uterus / Q000378:metabolism*
D046911:Macromolecular Substances; D011960:Receptors, Estrogen; D004958:Estradiol; D014357:Trypsin
Caractérisation par électrophorèse en système non dénaturant de la forme "4 S-trypsine" du récepteur cytosoluble de l'oestradiol d'utérus de Veau