Isolation and identification of the Cmicron4-domain of IgM.
Intact Cmicron4-domain was isolated by molecular exclusion chromatography of reduced and alkylated Fc5 micron prepared by tryptic digestion at 60 degrees C of a monoclonal IgM. Two fragments were obtained of which one contained carbohydrate and the other none. These fragments were successfully separated by chromatography on insolubilised Concanavalin A. Cmicron4-domain was identified by means of amino acid sequence, amino acid composition, molecular weight and immunological analyses. It has a molecular weight of 14,700 daltons and results from tryptic cleavage at Lys-445 of the micron-chain.
D000595:Amino Acid Sequence; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D005779:Immunodiffusion; D007143:Immunoglobulin Heavy Chains / Q000302:isolation & purification; D007148:Immunoglobulin mu-Chains / Q000302:isolation & purification; D008970:Molecular Weight; D013329:Structure-Activity Relationship
D007143:Immunoglobulin Heavy Chains; D007148:Immunoglobulin mu-Chains