Characterization of 2-amino-2,6-dideoxy-D-glucose as a constituent of the lipopolysaccharide antigen of Pseudomonas aeruginosa immunotype 4.
The title lipopolysaccharide was freed from its lipid A component by mild, acid hydrolysis, to give a polysaccharide fraction that was subsequently hydrolyzed completely to afford a mixture of neutral sugars and amino sugars. The amino sugars were separated, and identified as 2-amino-2-deoxy-D-galactose, 2-amino-2,6-dideoxy-galactose as a 2:1 mixture of the D and L enantiomers, and 2-amino-2,6-dideoxy-D-glucose. A reference sample of 2-amino-2,6-dideoxy-D-glucose was synthesized by an improved preparative route. Among the lipopolysaccharide antigens of the seven recognized immunotypes of Pseudomonas aeruginosa, 2-amino-2,6-dideoxyglucose is also characterized as a constituent of two others, types 3 and 5.
D003847:Deoxyglucose / Q000031:analogs & derivatives / Q000032:analysis / Q000138:chemical synthesis; D005944:Glucosamine / Q000031:analogs & derivatives* / Q000032:analysis / Q000138:chemical synthesis; D008070:Lipopolysaccharides / Q000276:immunology; D009904:Optical Rotation; D011135:Polysaccharides, Bacterial / Q000276:immunology; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D013045:Species Specificity; D014961:X-Ray Diffraction
D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial; D003847:Deoxyglucose; D005944:Glucosamine