Pancreatic islets subjected to different concentrations of glucose in vitro. A study with special regard to mitochondrial changes.
Isolated pancreatic islets of mice and gerbils were cultured for 6 days at low (2mM) or high (20mM) concentrations of glucose after which they were studied using qualitative and quantitative electron microscopy, histo- and microchemistry, and X-ray microanalysis. Compared with the islets cultured at high glucose, those subjected to low glucose exhibited enhanced succinate dehydrogenase activity, a decreased content of adenosine triphosphate, and an increased volume of B-cell mitochondria which often were rounded or oval.
D000255:Adenosine Triphosphate / Q000032:analysis; D000818:Animals; D002118:Calcium / Q000032:analysis; D005260:Female; D005849:Gerbillinae; D005947:Glucose / Q000494:pharmacology*; D066298:In Vitro Techniques; D007515:Islets of Langerhans / Q000032:analysis / Q000187:drug effects* / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008854:Microscopy, Electron; D008928:Mitochondria / Q000648:ultrastructure*; D008933:Mitochondrial Swelling; D013385:Succinate Dehydrogenase / Q000032:analysis
D000255:Adenosine Triphosphate; D013385:Succinate Dehydrogenase; D005947:Glucose; D002118:Calcium