title: [Rapid freezing of biologic tissue. Measurement of temperature and rate of freezing by thin-layer thermocouple].
abstract: An apparatus for rapid freezing of biological tissues by contact with a copper block cooled by liquid helium has been devised to reduce the contamination of copper block surface. It prevents the precooling of the specimen while going through the layers of cold helium gas surrounding the copper block and reduces the quantity of helium necessary for freezing. It also enables one to obtain easily reproducible results from freezing by immersion in liquid coolants. Freezing rates are measured directly, related to the specimen thickness, by a thin film thermocouple; its low thermal inertia gives speed measurements of the order of 100,000 degrees C/s.
mesh_terms: D000818:Animals; D003300:Copper; D004553:Electric Conductivity; D005615:Freezing; D005629:Frozen Sections; D006371:Helium; D008722:Methods; D008867:Microtomy; D009532:Nickel; D051381:Rats; D013696:Temperature; D013997:Time Factors
chemical_list: D006371:Helium; D003300:Copper; D009532:Nickel
vernacular_title: Congélation rapide de tissus biologiques. Mesure des températures et des vitesses de congélation par thermocouple en couche mince.