Structure and ribonucleoprotein staining of kinetochores of colchicine-treated HeLa cells.
HeLa cells treated with colchicine show a lengthening of kinetochores lacking an inner layer. Combined EDTA- and ribonuclease treatment (BERNHARD 1969) indicate the presence of ribonucleoprotein in the dense outer layer as well as in the surrounding fine fibrillar mass. Reconstructions from serial sections reveal a band-like enlargement of these layers around the primary constriction during the inhibition of mitosis by colchicine.
D002503:Centromere / Q000187:drug effects / Q000648:ultrastructure*; D002875:Chromosomes / Q000648:ultrastructure*; D003078:Colchicine / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D006367:HeLa Cells / Q000187:drug effects / Q000648:ultrastructure*; D008938:Mitosis / Q000187:drug effects; D009698:Nucleoproteins / Q000032:analysis*; D012260:Ribonucleases / Q000494:pharmacology; D012261:Ribonucleoproteins / Q000032:analysis*
D009698:Nucleoproteins; D012261:Ribonucleoproteins; D004492:Edetic Acid; D012260:Ribonucleases; D003078:Colchicine