Preparation of specific immune sera against rabbit skeletal, pig and human cardiac heavy meromyosins.
Antibodies were produced in guinea-pigs against heavy-meromyosin (HMM) and tested by immunodiffusion and microcomplement fixation (MCF). A similar response was obtained for HMM extracted either from human and pig left ventricles, or from rabbit white skeletal muscles. For each muscle, the antisera cross-react quantitatively with the native intact myosin and are highly specific. Thus, this procedure permits the preparation of muscle type-specific anti myosin antibodies, by a simpler means than those previously published.
D000818:Animals; D003168:Complement Fixation Tests; D006168:Guinea Pigs / Q000276:immunology; D006801:Humans; D007106:Immune Sera / Q000096:biosynthesis; D007114:Immunization; D005779:Immunodiffusion; D009132:Muscles; D009206:Myocardium; D015879:Myosin Subfragments / Q000276:immunology* / Q000302:isolation & purification; D009218:Myosins; D011817:Rabbits; D013045:Species Specificity; D013552:Swine; D013997:Time Factors; D014357:Trypsin
D007106:Immune Sera; D015879:Myosin Subfragments; D014357:Trypsin; D009218:Myosins