Properties of derivatives of the Pseudomonas plasmid pVS1 that have inherited carbenicillin resistance from RP1.
A procedure is described for the isolation, in Pseudomonas aeruginosa PAO, of bacteria carrying derivatives of pVS1 that inherited the carbenicillin-resistance determinant from RP1 either alone or together with that for aeruginocin resistance. Such bacteria occur among the transconjugant progeny from both recombination-proficient or -deficient pVS1+ RP1+ donors, suggesting that the formation of these plasmids is due to the translocation of TnA from RP1 into pVS1. It is possible, therefore, that the aeruginocin-resistance determinant is part of TnA or is closely linked to it. Unexpectedly, none of these plasmids showed the 3 x 10(6)- to 4 x 10(6)-dalton increase in size predicted for TnA+ derivatives of PVS1. It is suggested that an interaction between TnA and the Tn501 translocation unit in pVS1 could account for this result.
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002228:Carbenicillin / Q000494:pharmacology*; D003227:Conjugation, Genetic; D004269:DNA, Bacterial; D004270:DNA, Circular; D005111:Extrachromosomal Inheritance; D010403:Penicillin Resistance; D010957:Plasmids; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D011995:Recombination, Genetic
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D004270:DNA, Circular; D002228:Carbenicillin