Biosynthesis of thiamin in Bacillus subtilis. Isolation of mutants accumulating 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate.
Thiamin-deficient mutants of Bacillus subtilis were characterized by their growth responses to the pyrimidine and thiazole moieties of the vitamin molecule and by cross-feeding tests. All mutants growing on the thiazole moiety and all mutants with an absolute requirement for thiamin fed all those growing on the pyrimidine moiety. No other cross-feeding effects were observed. From the culture fluid of a mutant growing on the thiazole moiety, two compounds were isolated which supported growth of mutants requiring the pyrimidine moiety. These compounds were identified by chromatographic, bioautographic and spectrophotometric procedures as 4-amino-5-hydroxymethyl-2-methylpyrimidine and its monophosphate derivative.
D001412:Bacillus subtilis / Q000235:genetics / Q000378:metabolism*; D002854:Chromatography, Paper; D009154:Mutation; D011743:Pyrimidines / Q000378:metabolism; D013831:Thiamine / Q000096:biosynthesis* / Q000378:metabolism; D013844:Thiazoles / Q000378:metabolism
D011743:Pyrimidines; D013844:Thiazoles; D013831:Thiamine