Enzymatic assay for the antitumor agent-N-(phosphonacetyl)-L-aspartic acid (PALA).
A method is described for the assay of N-(phosphonacetyl)-L-aspartic acid in plasma or urine, based on inhibition of partially purified aspartate carbamoyltransferase from rat liver. Concentrations of N-(phosphonacetyl)-L-aspartic acid as low as 0.1 microgram/ml can be detected. Plasma disappearance and urinary excretion curves from one patient are shown as examples of the application of the method.
D000818:Animals; D001221:Aspartate Carbamoyltransferase / Q000037:antagonists & inhibitors*; D001224:Aspartic Acid / Q000031:analogs & derivatives* / Q000032:analysis / Q000494:pharmacology; D001681:Biological Assay; D006801:Humans; D066298:In Vitro Techniques; D008722:Methods; D009943:Organophosphorus Compounds / Q000032:analysis*; D010746:Phosphonoacetic Acid / Q000031:analogs & derivatives / Q000032:analysis* / Q000494:pharmacology; D051381:Rats; D013997:Time Factors
D009943:Organophosphorus Compounds; D001224:Aspartic Acid; D001221:Aspartate Carbamoyltransferase; D010746:Phosphonoacetic Acid