Chemical transformation of human revertant cells induced by murine sarcoma virus.
A human revertant cell line, derived from non-producer human osteosarcoma cells (NP/KHOS) induced by Kirsten murine sarcoma virus, was treated in vitro with various levels of polycyclic aromatic hydrocarbons (3-methylcholanthrene, 7,12-dimethylbenz(alpha)anthracene, and benzo(alpha)pyrene [BP] or dimethyl sulfoxide (control). Cells treated only with 3-methylcholanthrene and 7,12-dimethylbenz(alpha)anthracene underwent morphological alteration in vitro, and produced tumors when injected into NIH nude mice. These human revertant cells may be a useful in vitro tool in screening for the potential chemical carcinogens in human cell systems.
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000494:pharmacology; D000362:Agar; D001580:Benzopyrenes / Q000494:pharmacology; D002273:Carcinogens; D002449:Cell Aggregation / Q000187:drug effects; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced*; D002472:Cell Transformation, Viral; D003601:Cytotoxicity Tests, Immunologic; D006801:Humans; D008748:Methylcholanthrene / Q000494:pharmacology; D011083:Polycyclic Compounds / Q000494:pharmacology*
D001580:Benzopyrenes; D002273:Carcinogens; D011083:Polycyclic Compounds; D008748:Methylcholanthrene; D015127:9,10-Dimethyl-1,2-benzanthracene; D000362:Agar