Nucleotide sequence of a lysine tRNA from Bacillus subtilis.
A lysine tRNA (tRNA1Lys) was purified from Bacillus subtilis W168 by a consecutive use of several column chromatographic systems. The nucleotide sequence was determined to be pG-A-G-C-C-A-U-U-A-G-C-U-C-A-G-U-D-G-G-D-A-G-A-G-C-A-U-C-U-G-A-C-U-U(U*)-U-U-K-A-psi-C-A-G-A-G-G-m7G(G)-U-C-G-A-A-G-G-T-psi-C-G-A-G-U-C-C-U-U-C-A-U-G-G-C-U-C-A-C-C-AOH, where K and U* are unidentified nucleosides. The nucleosides of U34 and m7G46 were partially substituted with U* and G, respectively. The binding ability of lysyl-tRNA1Lys to Escherichia coli ribosomes was stimulated with ApApA as well as ApApG.
D001412:Bacillus subtilis / Q000032:analysis*; D001483:Base Sequence; D008239:Lysine; D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides / Q000032:analysis; D012343:RNA, Transfer; D012263:Ribonucleosides / Q000032:analysis
D009843:Oligoribonucleotides; D012263:Ribonucleosides; D012343:RNA, Transfer; D008239:Lysine