Studies on the coupling rates in liquid-phase peptide synthesis using competition experiments.
Competition experiments were carried out in order to determine relative reaction rates, Vrel, for the peptide-forming step. Using the liquid-phase method of peptide synthesis a model system was developed to investigate the influence of coupling method, amino component, derivatization and solvent upon Vrel. According to a standard procedure, Vrel for all 20 commonly occurring amino acids were established. A strong dependence of Vrel upon steric effects of the coupling components was found. The data obtained may serve as a guideline for optimizing the reaction conditions in peptide synthesis.
D000596:Amino Acids / Q000032:analysis; D055598:Chemical Phenomena; D002621:Chemistry; D007202:Indicators and Reagents; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis / Q000138:chemical synthesis*
D000596:Amino Acids; D007202:Indicators and Reagents; D010455:Peptides