The use of antibody-coated liposomes as a target cell model for antibody-dependent cell-mediated cytotoxicity.
A simple model system was developed for antibody-dependent cell-mediated cytotoxicity (ADCC) using antibody-coated synthetic membranes (liposomes) as a target cell model. A synthetic hapten, dinitrophenyl-phosphatidylethanolamine (DNP-PE), was incorporated into fluorophore-quencher-loaded liposomes and the latter were coated with pure anti-DNP antibodies. Normal spleen lymphocytes were capable of binding and subsequently lysing these liposomes. This process is dependent upon the presence of an intact (Fc-containing) IgG molecule and independent of exogenous serum complement. The effector lymphocytes are nylon non-adherent, devoid of Thy-1 antigen and present in nude mice, suggesting an identity with K (Fc receptor positive) lymphocytes. These studies indicate that liposomes may be used as a model to study the requirements for the binding and lysis of target cells in this cell-mediated cytotoxic system.
D000818:Animals; D000920:Antibody-Dependent Cell Cytotoxicity; D002462:Cell Membrane / Q000276:immunology; D003601:Cytotoxicity Tests, Immunologic / Q000379:methods; D004136:Dinitrobenzenes / Q000276:immunology; D006241:Haptens / Q000276:immunology; D007141:Immunoglobulin Fc Fragments / Q000276:immunology; D007074:Immunoglobulin G / Q000032:analysis; D008081:Liposomes / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000166:cytology
D004136:Dinitrobenzenes; D006241:Haptens; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D008081:Liposomes