title: Resolution of the glycosyltransferase activities from two strains of Streptococcus mutans by polyacrylamide gel electrophoresis in the presence of Tween 80.
abstract: The glycosyltransferase complex from Streptococcus mutans can be resolved by polyacrylamide gel electrophoresis following treatment with Tween 80. The enzyme complex was treated with Tween 80 and electrophoresis was performed in the presence of Tween 80. Discrete bands of glycosyltransferase activity were observed when the gels were incubated in buffered sucrose. The enzyme aggregation that occurs with the exocellular glycosyltransferase may involve lipophilic interactions that are disrupted by the Tween 80.
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D006602:Hexosyltransferases / Q000302:isolation & purification*; D011136:Polysorbates; D013045:Species Specificity; D013291:Streptococcus / Q000201:enzymology*
chemical_list: D011136:Polysorbates; D006602:Hexosyltransferases