title: Comparison of estimates of gonadotropin levels by isolated blood samples, integrated blood concentrations, and timed urinary fractions.
abstract: Gonadotropin levels in isolated blood samples, integrated plasma concentrations (IC), and timed urinary collections have been compared in 5 males with delayed puberty and 7 normal adult males. There was a significant correlation between urinary levels in 24-h collection and those in each of four shorter timed collections for both LH and FSH. Similarly, 24-h integrated plasma concentration and 4-h (0800--1200 h) integrated plasma concentration obtained on 10 additional subjects showed significant correlation. The 4-h integrated plasma concentrations correlated with single blood samples or the mean of three samples obtained at 0800, 1200, and 1600 h. These 4-h plasma samples also correlated significantly with all urine collections for FSH but only with the 2200--0800 h urine collection for LH. The study suggests that LH and FSH levels in urine samples collected over several hours correlate with 24-h urinary excretion and that levels in single blood samples estimate the 24-h plasma integrated concentration.
mesh_terms: D000293:Adolescent; D000328:Adult; D001800:Blood Specimen Collection; D004700:Endocrine System Diseases / Q000097:blood / Q000652:urine; D005640:Follicle Stimulating Hormone / Q000097:blood / Q000652:urine*; D006801:Humans; D007986:Luteinizing Hormone / Q000097:blood / Q000652:urine*; D008297:Male; D011627:Puberty; D013048:Specimen Handling; D013997:Time Factors
chemical_list: D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone