Activation of a new plasma enzyme by antigen-antibody reaction.
The change of enzyme activity in immunized rabbit plasma after addition of the homologous antigen was examined. The activities of N alpha-tosyl-L-arginine methyl ester (TAMe) and N alpha-tosyl-L-lysine methyl ester (TLMe) hydrolysis increased about 15 to 18 days after immunization. This increase was especially marked before the maximal rise of antibody content, and is thought to be related to the IgM antibody not to the IgG antibody. Enzyme activation was strongly inhibited by chelation of Ca2+ with 5 mM disodium ethylenediamine tetraacetate (EDTA), but not by other protease inhibitors, such as epsilon amino-caproic acid (epsilon-ACA), bovine lung kallikrein inhibitor (Trasylol) or soybean trypsin inhibitor (SBTI).
D000818:Animals; D000937:Antigen-Antibody Reactions; D002118:Calcium / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D004527:Egg Proteins / Q000276:immunology / Q000494:pharmacology; D004789:Enzyme Activation; D004950:Esterases / Q000097:blood*; D007114:Immunization; D007075:Immunoglobulin M / Q000378:metabolism; D008239:Lysine / Q000031:analogs & derivatives; D011817:Rabbits; D014106:Tosylarginine Methyl Ester
D004527:Egg Proteins; D007075:Immunoglobulin M; D014106:Tosylarginine Methyl Ester; D004492:Edetic Acid; D004950:Esterases; D008239:Lysine; D002118:Calcium