Conservation of active ribosomes in acetone-treated cells of Tetrahymena pyriformis.
The preparation of an acetone powder of cells of Tetrahymena pyriformis GL is described. A comparison of ribosomal particles isolated from acetone-treated and untreated cells shows that structurally and functionally intact ribosomes can be isolated from acetone-treated cells. Fully active ribosomes have been isolated from acetone powder of Tetrahymena that had been stored for more than 6 months at 4 degrees C. Thus, this procedure allows easy storage of large quantities of cells for the bulk preparation of active ribosomes.
D000096:Acetone; D000818:Animals; D002458:Cell Fractionation / Q000379:methods; D008970:Molecular Weight; D014176:Protein Biosynthesis; D012269:Ribosomal Proteins / Q000032:analysis; D012270:Ribosomes / Q000378:metabolism / Q000648:ultrastructure*; D013769:Tetrahymena pyriformis / Q000378:metabolism / Q000648:ultrastructure*
D012269:Ribosomal Proteins; D000096:Acetone