Antipyrine plasma half-life. In vivo indicator of oxidative metabolic capability in Rhesus monkeys (Macaca mulatta).
Antipyrine plasma half-life (APH) has been used as an indicator of hepatic oxidase activity in rhesus monkeys (Macaca mulatta). Plasma disappearance of 14CH3-N-antipyrine (AP) was measured radiometrically. AP and 3 metabolites were detected using high pressure liquid chromatography. APH was assessed during a 300-day control period and following phenobarbital (PB) pretreatments. Significant interindividual variability was observed requiring that animals be used as their own controls. PB (15 mg/kg i.m., 2/day X 4 days) reduced mean APH values from 81 +/- 12 to 41 +/- 2 min. PB treatment also increased monooxygenase catalyzed aldrin epoxidation, dihydroisodrin hydroxylation and benzo(a)pyrene oxidation measured using liver biopsy homogenates. These parameters permit 'continual' assessment of HMO activity in rhesus monkeys under a variety of experimental conditions.
D000818:Animals; D000983:Antipyrine / Q000097:blood* / Q000494:pharmacology; D002853:Chromatography, Liquid; D006207:Half-Life; D000882:Haplorhini; D008099:Liver / Q000201:enzymology*; D008253:Macaca mulatta; D008297:Male; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism*; D010634:Phenobarbital / Q000494:pharmacology; D013997:Time Factors
D010088:Oxidoreductases; D000983:Antipyrine; D010634:Phenobarbital