[Isatin-enzyme interactions. VI. Inhibition of rat kidney alkaline phosphatase].
Interaction of isatin with rat kidney alkaline phosphatase has been studied. Mode of attachment of isatin with the enzyme protein is most likely through amino group(s), which is also imperative for catalysis. Sulphydryl group(s) do not seem to be involved in enzyme action. Zinc is also needed for enzyme activity. Use of sulphydryl compounds suggests that isatin inhibition of the enzyme is through attachment at the metal site. However, this inhibition may not only be due to simple chelation of the metal by isatin.
D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors*; D000588:Amines; D000818:Animals; D001665:Binding Sites; D003545:Cysteine / Q000494:pharmacology; D004492:Edetic Acid / Q000494:pharmacology; D005978:Glutathione / Q000494:pharmacology; D007211:Indoles / Q000494:pharmacology*; D007510:Isatin / Q000494:pharmacology*; D007668:Kidney / Q000201:enzymology; D008297:Male; D008623:Mercaptoethanol / Q000494:pharmacology; D051381:Rats; D013438:Sulfhydryl Compounds; D015032:Zinc / Q000494:pharmacology
D000588:Amines; D007211:Indoles; D013438:Sulfhydryl Compounds; D008623:Mercaptoethanol; D007510:Isatin; D004492:Edetic Acid; D000469:Alkaline Phosphatase; D005978:Glutathione; D015032:Zinc; D003545:Cysteine