[Fragmentation of DNA of monkey adenovirus type 7 by specific endonucleases R. SalI and R. BamI].
The effect of restrictive endonucleases R. Ecor I, R. Bam I, and R. Sal I on simian adenovirus type 7 (SA7) genome was studied. Because of the occurrence of the only recognition site, the R. EcoR I enzyme cuts SA7 DNA into two fragments with molecular mass of 12 and 10 X 10(6) daltons. R. Ram I cuts this DNA in 6 sites producing 7 fragments with molecular mass 6.6, 5.9, 3.8, 2.7, 1.3, 0.7 and 0.6 X 10(6) daltons. Treatment with R. Sal I gives 6 fragments with molecular mass of 8.1, 5.5, 4.3, 2.45, 1.2, and 0.6 X 10(6) daltons R. Bam I and R. Sal I fragments form the sequence E--A--D--F--C--G--B and A--B--D--F--E--C, respectively. The only recognition site of R. EcoR I is localized in the C fragment of R. Bam I and B fragment of R. Sal I.
D000256:Adenoviridae / Q000235:genetics; D000261:Adenoviruses, Simian / Q000235:genetics; D000818:Animals; D005591:Chemical Fractionation; D006224:Cricetinae; D004279:DNA, Viral / Q000032:analysis*; D004587:Electrophoresis, Agar Gel; D004720:Endonucleases / Q000494:pharmacology; D004926:Escherichia coli / Q000201:enzymology*; D005814:Genes, Viral / Q000187:drug effects; D000882:Haplorhini; D006868:Hydrolysis; D008970:Molecular Weight
D004279:DNA, Viral; D004720:Endonucleases
Fragmentatsiia DNK adenovirusa obez'ian tipa 7 spetsificheskimi endonukleazami R SalI i R. BamI.