[Radioimmunoassay of hormonal activity of Gobius niger L. testicular interstitial tissue in organ culture: spontaneous activity and stimulation by pituitary gland].
After the interstitial tissue was cultivated, the radio-immunoassay revealed in the media very small quantities of free testosterone but much larger quantities of testosterone conjugates as glucuronids and sulfates. Association of pituitaries increases greatly the secretion. 12-13 days precultivated pituitaires retain close to 70% of their stimulating potency.
D000818:Animals; D005399:Fishes; D005965:Glucuronates / Q000378:metabolism; D008297:Male; D009924:Organ Culture Techniques; D010902:Pituitary Gland / Q000502:physiology*; D011863:Radioimmunoassay; D013737:Testis / Q000378:metabolism*; D013739:Testosterone / Q000378:metabolism*
D005965:Glucuronates; D013739:Testosterone
Etude par dosage radio-immunologique de l'activité hormonale du tissu interstitiel du testicule de Gobius niger L. en culture organotypique: activité spontanée et stimulation hypophysaire.