Evidence for cycloheximide acting as a glutamine analogue in plant tissue.
In growing maize root tissue [14C]asparagine formation in inhibited and [14C]glutamine accumulation stimulated by treatment with cycloheximide or glutamine analogs such as azaserine. In contrast, puromycin enhances the accumulation of [14C]asparagine but not [14C]glutamine. Cycloheximide and puromycin alone inhibit protein synthesis. This is interpreted to mean that the alteration in amide metabolism following cycloheximide treatment is a direct result of the antibiotic acting as a glutamine analog. While cycloheximide is often the cytoplasmic protein synthesis inhibitor of choice due to its potency and rapid action, its assumed specificity of action of eukaryotes is doubtful.
D001216:Asparagine / Q000096:biosynthesis; D001377:Azaserine / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology*; D004492:Edetic Acid / Q000494:pharmacology; D005973:Glutamine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D010944:Plants / Q000187:drug effects / Q000378:metabolism*; D014176:Protein Biosynthesis / Q000187:drug effects; D011691:Puromycin / Q000494:pharmacology; D003313:Zea mays / Q000378:metabolism
D005973:Glutamine; D011691:Puromycin; D001216:Asparagine; D001377:Azaserine; D003513:Cycloheximide; D004492:Edetic Acid