Immunochemical analysis of the N-acetyl hexosaminidases in human-mouse hybrids made using a double selective system.
A human-mouse hybrid, DUR 4 (Solomon et al., 1976), containing a human X/15 translocation chromosome and also chromosome 5, among other human chromosomes, was used in a double selection system to obtain hybrids of four different types: X/15+ 5+, X/15- 5+, X/15+, 5-. Standard positive and negative selection systems were used for the X chromosome, and negative selection for chromosome 5 was done with diphtheria toxin. The assignment of HEXB and presently only when both the X/15 chromosome and chromosome 5 were present. A "HEXA-like" band segregated with chromosome 15 (or X/15) but independently of chromosome 5. This component, unlike HEXA, does not contain human HEXbeta antigen.
D000818:Animals; D002478:Cells, Cultured; D002999:Clone Cells; D004592:Electrophoresis, Starch Gel; D005347:Fibroblasts / Q000201:enzymology / Q000276:immunology; D006596:Hexosaminidases / Q000032:analysis*; D006801:Humans; D006822:Hybrid Cells / Q000201:enzymology* / Q000276:immunology; D005779:Immunodiffusion; D051379:Mice; D014178:Translocation, Genetic
D006596:Hexosaminidases