The subunit and polypeptide-chain structure of rabbit secretory immunoglobulin A. Isolation of a proteolytic fragment suitable for sequence studies on the variable region of alpha-chain.
A method was developed for the preparation of a proteolytic fragment of rabbit secretory immunoglobulin A (sIgA) which contains the variable region of the alpha-chain; this fragment is suitable for primary-sequence studies. The serologically defined subclasses of sIgA are shown to correlate partially with the nature of the binding of a constituent chain of sIgA, called secretory piece. Data are also presented on the relative resistance of sIgA to enzymic and reductive cleavage, compared with immunoglobulin G.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001666:Binding Sites, Antibody / Q000302:isolation & purification*; D002848:Chromatography, DEAE-Cellulose; D004591:Electrophoresis, Polyacrylamide Gel; D007070:Immunoglobulin A; D007140:Immunoglobulin Fab Fragments; D007143:Immunoglobulin Heavy Chains / Q000302:isolation & purification*; D007144:Immunoglobulin J-Chains; D007135:Immunoglobulin Variable Region / Q000302:isolation & purification*; D007137:Immunoglobulin alpha-Chains / Q000302:isolation & purification*; D007142:Immunoglobulin gamma-Chains; D011817:Rabbits; D012635:Secretory Component
D000596:Amino Acids; D007070:Immunoglobulin A; D007140:Immunoglobulin Fab Fragments; D007143:Immunoglobulin Heavy Chains; D007144:Immunoglobulin J-Chains; D007135:Immunoglobulin Variable Region; D007137:Immunoglobulin alpha-Chains; D007142:Immunoglobulin gamma-Chains; D012635:Secretory Component