Competition for L-glutamate between specialised and versatile Clostridium species.
Clostridium cochlearium could be reproducibly enriched in an L-aspartate- and L-glutamate-limited, anaerobic chemostat inoculated with anaerobic sludge. L-glutamate, L-glutamine and L-histidine were the only fermentable substrates. Less specialised clostridia of the C. tetanomorphum type could only be isolated from batch enrichments with L-glutamate and L-aspartate as energy sources. Competition experiments with C. cochlearium and C. tetanomorphum in a L-glutamate-limited chemostat resulted in the selective elimination of the latter species. Addition of glucose to the medium resulted in coexistence of both species. The molar growth yields for L-glutamate at different dilution rates at 30 degrees C were determined for both species. The maximum specific growth rates on L-glutamate were 0.55 h-1 for C. cochlearium and 0.35 h-1 for C. tetanomorphum.
D000693:Anaerobiosis; D000898:Antibiosis; D001224:Aspartic Acid / Q000378:metabolism; D003013:Clostridium / Q000254:growth & development / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D005971:Glutamates / Q000378:metabolism*; D012722:Sewage; D013045:Species Specificity; D013237:Stereoisomerism; D014871:Water Microbiology
D005971:Glutamates; D012722:Sewage; D001224:Aspartic Acid; D005947:Glucose