Mannose-binding hemagglutinins in extracts of Pseudomonas aeruginosa.
Mannose-binding hemagglutinins were found in the extracts of a pyocyanin-forming Pseudomonas aeruginosa, which contain galactose-specific hemagglutinins. They were purified simultaneously with the latter proteins by heating to 70 degrees C, precipitating with ammonium sulfate, application to a Sepharose 4B column, and elution from it by 0.05 M mannose. The mannose-specific hemagglutinins were shown to be similar to the galactophilic ones in (a) being glycoproteins of very low molecular weight (about 11 000 by SDS gel electrophoresis), (b) their tendency to aggregate, and (c) their ability to effect stronger agglutination of erythrocytes treated with papain than of untreated ones. They were found to resemble them also in their reaction with simple sugars and interactions with divalent cations, which are essential for their activity. In these properties, as well as in their relative resistance to heat and to proteolytic enzymes, these two types of bacterial hemagglutinins are like most of the plant, contrasted with the animal, hemagglutinins. The reactions with mannose and mannose-bearing compounds (yeast mannan, horseradish peroxidase (EC 1.11.1.7), and serum globulins), which are not shared with the galactophilic Pseudomonas hemagglutinins, indicate a relationship of the mannose-binding protein of Pseudomonas to the plant lectin concanavalin A. The mannose-binding hemagglutinins do not exhibit identical cell-agglutinating spectra owing to difference in profiles of sugar specificity and relative affinity to mannose derivatives compared with free mannose.
D000373:Agglutinins / Q000378:metabolism*; D000596:Amino Acids / Q000032:analysis; D006388:Hemagglutinins / Q000302:isolation & purification / Q000378:metabolism*; D006735:Horseradish Peroxidase / Q000378:metabolism; D006358:Hot Temperature; D037102:Lectins / Q000378:metabolism; D008351:Mannans / Q000378:metabolism; D008358:Mannose; D011550:Pseudomonas aeruginosa
D000373:Agglutinins; D000596:Amino Acids; D006388:Hemagglutinins; D037102:Lectins; D008351:Mannans; D006735:Horseradish Peroxidase; D008358:Mannose