The extraction and partial characterization of proteins released by decalcification from calcified human aortic plaques.
Tissue from severely atherosclerotic human aortas was divided into heavily calcified (plaque) and less calcified (non-plaque) areas. After defatting and thorough extraction with neutral salt solutions, further proteinaceous material was released from plaque than non-plaque areas. Although organic phosphorous was associated with the EDTA-solubilized protein from both plaque and non-plaque areas, the phosphorous to protein ratio of extracts from plaque areas was 2 to 3 times that from non-plaque areas. Analysis of the major protein component of the extract of plaque areas indicated an acidic glyco-protein which appeared to be associated with an organic phosphorous moiety as well as cholesterol and cholesterol esters.
D000596:Amino Acids / Q000032:analysis; D001011:Aorta / Q000032:analysis / Q000378:metabolism*; D001161:Arteriosclerosis / Q000378:metabolism*; D002114:Calcinosis / Q000378:metabolism*; D002784:Cholesterol / Q000032:analysis; D002788:Cholesterol Esters / Q000032:analysis; D004492:Edetic Acid; D006023:Glycoproteins / Q000032:analysis; D006801:Humans; D008903:Minerals / Q000032:analysis; D009943:Organophosphorus Compounds / Q000032:analysis; D010743:Phospholipids / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D012995:Solubility
D000596:Amino Acids; D002788:Cholesterol Esters; D006023:Glycoproteins; D008903:Minerals; D009943:Organophosphorus Compounds; D010743:Phospholipids; D011506:Proteins; D002784:Cholesterol; D004492:Edetic Acid