Deviated lysis (d.l.): III. Kinetics of interaction of d.l. activity with chicken erythrocytes: evidence for E formation.
The interaction of d.l. activity with chicken red cells (CE) generates a cell intermediate with the properties of classical E*. Generation of CE* by d.l. activity at 37 degrees C is rapid, while there is a considerable lag in the conversion of CE* to ghost and hemoglobin. Conversion of CE* to ghosts can be blocked by high concentration of EDTA and/or 0 degrees C. CE* contain at least C6 and C9 on their surface.
D000818:Animals; D003183:Complement C6 / Q000378:metabolism; D003185:Complement C8 / Q000378:metabolism; D003186:Complement C9 / Q000378:metabolism; D003165:Complement System Proteins / Q000378:metabolism*; D004492:Edetic Acid / Q000494:pharmacology; D004912:Erythrocytes / Q000276:immunology*; D006461:Hemolysis; D007444:Inulin / Q000494:pharmacology; D007700:Kinetics; D013696:Temperature
D003183:Complement C6; D003185:Complement C8; D003186:Complement C9; D007444:Inulin; D003165:Complement System Proteins; D004492:Edetic Acid