Improved synthesis of CMP-sialates using enzymes from frog liver and equine submandibular gland.
An efficient method for the preparation of CMP-N-acetylneuraminic acid using crude or partially purified CTP:N-acylneuraminate cytidylyltransferase from equine submandibular gland or frog liver is described. The yield of the sugar nucleotide after purification by ion-exchange chromatography and gel filtration was 95%. The compound was studied by 360 MHz 1H NMR spectroscopy in addition to the usual chemical and physical analyses. The preparation of radioactive or unlabelled CMP 4-O-methyl-N-acetylneuraminic acid, which is not known to occur in nature, was achieved in 17% yield with the aid of the equine enzyme.
D000818:Animals; D001001:Anura; D003569:Cytidine Monophosphate N-Acetylneuraminic Acid / Q000096:biosynthesis*; D006736:Horses; D007700:Kinetics; D008099:Liver / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008623:Mercaptoethanol / Q000494:pharmacology; D012794:Sialic Acids / Q000096:biosynthesis*; D012799:Sialyltransferases / Q000378:metabolism; D013363:Submandibular Gland / Q000378:metabolism*
D012794:Sialic Acids; D003569:Cytidine Monophosphate N-Acetylneuraminic Acid; D008623:Mercaptoethanol; D012799:Sialyltransferases