Acid hydrolases in normal B and T blood lymphocytes.
B and T lymphocytes were separated by means of the spontaneous sheep red blood cell rosette formation technique from 3 normal donors. The following acid hydrolases were biochemically determined on separated B and T lymphocytes: acid phosphatase, beta-glucuronidase, beta-galactosidase, beta-hexosaminidase, alpha-arabinosidase, alpha-galactosidase, alpha-mannosidase, alpha-glucosidase, and pH 4.0 and pH 5.0 beta-glucosidase. The activities of most of the acid hydrolases including acid phosphatase and beta-glucuronidase were found to be slightly decreased in B lymphocytes when compared to T lymphocytes. However, alpha-mannosidase activity was found to be significantly higher in the B lymphocytes than in the T lymphocytes and offers the possibility of using this enzyme as a B lymphocyte marker.
D001402:B-Lymphocytes / Q000201:enzymology* / Q000276:immunology; D006801:Humans; D006867:Hydrolases / Q000032:analysis*; D012397:Rosette Formation; D013601:T-Lymphocytes / Q000201:enzymology* / Q000276:immunology
D006867:Hydrolases