Influence of anti-rheumatic drugs on human lymphocytes in vitro.
Influence of anti-rheumatic drugs on human lymphocytes, especially T and B cell membranes, was studies with D-penicillamine, aurothiomalate, dexamethasone, cyclophosphamide, mitomycin C and aspirin.


METHODS
Peripheral blood obtained from five healthy individuals and lymphocytes were separated by centrifugation with Lymphoprep. The separated lymphocytes were adjusted to 5 X 10(6)/ml in PBS. The suspension of lymphocytes was mixed with equal volume of each concentration of the above drugs. After suspensions, we investigated the percentages of T -and B-cells, compared to control. The results are as follows: 1. Drugs which act only on the T cell membrane: D-penicillamine, aurothiomalate. 2. Drug which acts only on B cell membrane: dexamethasone. 3. Drugs which act on both T- and B-cell membrane: mitomycin C, cyclophosphamide and aspirin.
D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001172:Arthritis, Rheumatoid / Q000188:drug therapy*; D001241:Aspirin / Q000494:pharmacology; D001402:B-Lymphocytes / Q000187:drug effects; D003520:Cyclophosphamide / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical / Q000379:methods; D006052:Gold Sodium Thiomalate / Q000494:pharmacology; D006801:Humans; D007958:Leukocyte Count; D008214:Lymphocytes / Q000187:drug effects*; D008937:Mitomycins / Q000494:pharmacology; D010396:Penicillamine / Q000494:pharmacology; D013601:T-Lymphocytes / Q000187:drug effects
D000893:Anti-Inflammatory Agents; D008937:Mitomycins; D006052:Gold Sodium Thiomalate; D003907:Dexamethasone; D003520:Cyclophosphamide; D010396:Penicillamine; D001241:Aspirin