Disulfide bonding of secretory component to a single monomer subunit in human secretory IgA.
The arrangement of disulfide bonds joining secretory component (SC) to the alpha chains in secretory IgA was studied by determining the molecular size of the principal fragments resulting from CNBr digestion of secretory dimeric Fc fragments from IgA (Fc)2alpha fragments). In vitro complexes formed by incubating 125I-free SC and myeloma 131I-(Fc)2alpha fragments were isolated by gel filtration and subsequently digested with cyanogen bromide. The CNBr digests of SC-(Fc)2alpha fragments were analyzed by gel filtration in 5 M guanidine. Two principal fragments were obtained, one containing a monomeric Fc fragment from IgA (Fcalpha) associated with SC (m.w. congruent to 110,000) and a second containing the second Fcalpha monomer (m.w. congruent to 50,000) from the dimeric SC-(Fc)2alpha. Similar results were obtained when secretory (Fc)2alpha fragments isolated from native secretory IgA dimer were subjected to CNBr digestion. The data indicate that SC is disulfide bonded to a single monomer subunit in secretory IgA dimer.
D055598:Chemical Phenomena; D002621:Chemistry; D003488:Cyanogen Bromide; D004220:Disulfides; D006801:Humans; D007070:Immunoglobulin A / Q000302:isolation & purification; D007071:Immunoglobulin A, Secretory / Q000302:isolation & purification; D007141:Immunoglobulin Fc Fragments / Q000302:isolation & purification; D007128:Immunoglobulin Fragments; D007143:Immunoglobulin Heavy Chains / Q000302:isolation & purification; D007137:Immunoglobulin alpha-Chains / Q000302:isolation & purification; D008970:Molecular Weight; D010447:Peptide Hydrolases; D012635:Secretory Component
D004220:Disulfides; D007070:Immunoglobulin A; D007071:Immunoglobulin A, Secretory; D007141:Immunoglobulin Fc Fragments; D007128:Immunoglobulin Fragments; D007143:Immunoglobulin Heavy Chains; D007137:Immunoglobulin alpha-Chains; D012635:Secretory Component; D010447:Peptide Hydrolases; D003488:Cyanogen Bromide