A rapid and sensitive method using immunological nephelometric analysis to evaluate the gel-filtration method. (Application to the diagnosis of rubella).
Immunological nephelometric analysis has been shown to be a more sensitive method than either immunoelectrophoresis or gel-diffusion for testing the gel-filtration method of human immunoglobulins sera. This method has the advantages of facility, rapidity and sensitivity. We have compared this method to radial immunodiffusion. This method can be applied to locate with precision any antigens after gel-filtration or ion-exchange chromatography.
D000888:Antibodies, Anti-Idiotypic; D000914:Antibodies, Viral / Q000302:isolation & purification*; D000937:Antigen-Antibody Reactions; D002850:Chromatography, Gel / Q000379:methods*; D005069:Evaluation Studies as Topic; D006801:Humans; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007074:Immunoglobulin G / Q000302:isolation & purification*; D007075:Immunoglobulin M / Q000302:isolation & purification*; D009391:Nephelometry and Turbidimetry; D012409:Rubella / Q000175:diagnosis*; D012412:Rubella virus / Q000276:immunology
D000888:Antibodies, Anti-Idiotypic; D000914:Antibodies, Viral; D007074:Immunoglobulin G; D007075:Immunoglobulin M