Proteinase and proteinase-inhibitor activities of rat uterine myometrium during pregnancy and involution.
A supernatant fraction was prepared from rat uterine myometrium by homogenization, sonication and centrifugation. In this supernatant the protein concentration and the activities of an acid proteinase, an acid phosphatase and a proteinase inhibitor were measured. From the fibrous sediment, after washing with 0.5% Triton X-100 and with water, an actomyosin-containing solution was obtained by extraction with 0.6M-NaCl, and in this extract the protein concentration and a neutral proteinase activity were measured. The myometrial wet weight and the activities of the acid proteinase, acid phosphatase and proteinase inhibitor increased by factors of 3-15 during pregnancy and decreased to the same or a greater extent during involution. The amount of protein extracted with 0.6M-NaCl increased by a factor of only 2.3 and the neutral proteinase activity remained essentially constant during pregnancy and involution. The pH optimum of the neutral proteinase, and its pattern of activity compared with those of the lysosomal enzymes, show that the neutral proteinase is not of lysosomal origin. Actomyosin is degraded by the neutral proteinase activity in vitro. Since actomyosin is rapidly broken down only after parturition, the action of the neutral proteinase activity on actomyosin, if this occurs in vivo, must be regulated in some way. The proteinase-inhibitor activity measured in the first supernatant varied in a manner which suggested that it could be involved in this control.
D000135:Acid Phosphatase / Q000378:metabolism; D000818:Animals; D005260:Female; D009124:Muscle Proteins / Q000378:metabolism; D009215:Myometrium / Q000033:anatomy & histology / Q000201:enzymology / Q000378:metabolism*; D009929:Organ Size; D010447:Peptide Hydrolases / Q000378:metabolism*; D049590:Postpartum Period; D011247:Pregnancy; D011270:Pregnancy, Animal; D011480:Protease Inhibitors / Q000378:metabolism*; D051381:Rats; D014599:Uterus / Q000378:metabolism*
D009124:Muscle Proteins; D011480:Protease Inhibitors; D000135:Acid Phosphatase; D010447:Peptide Hydrolases