Recovery of CFU-C after freezing of normal and leukemic human bone marrow.
Studies have been carried out to determine the viability of leukemic and normal human bone marrow, cryopreserved in liquid nitrogen at -196 degrees C, using changes in total cell numbers and granulocyte colony forming ability in vitro. These studies have shown that there is considerable variability in the recovery of CFU-C from individual specimens. When the overall recovery, in all patients, is taken into account, there is a gradual decline in CFU-C numbers to about 60% after 24 months of freezing. CFU-C recovery is closely correlated with recovery of total cell numbers.
D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation; D002470:Cell Survival; D003114:Colony-Forming Units Assay; D006801:Humans; D007938:Leukemia / Q000188:drug therapy / Q000473:pathology*; D009584:Nitrogen; D013997:Time Factors
D009584:Nitrogen