A method for preparing and handling cross-sections from early chick embryos for scanning electron microscopy.
A method is described for processing multiple cross-sections from early chick embryos for scanning electron microscopy. Embryos are cut through the desired regions. Sections are affixed to a coverslip with Duco cement and critical point dried by Freon 13 or liquid CO2. This method provides a reliable means for preparing multiple cross-sections from single embryos and eliminates the need for direct handling of brittle tissues after drying.
D000818:Animals; D001921:Brain / Q000648:ultrastructure; D002642:Chick Embryo; D006652:Histological Techniques; D008855:Microscopy, Electron, Scanning; D013048:Specimen Handling / Q000379:methods*