[Study of the binding of thyroxine by thyroxine-binding prealbumin by a dialysis method using a fixed free concentration of ligand (author's transl)].
In order to study ligand-protein binding in solution, a dialysis method was used in which the free concentration of ligand can be controlled. The method has certain advantages and was applied to the binding of thyroxine by thyroxine-binding prealbumin, a system about which the results found in the literature are not in good agreement. From the isotherm drawn according to the Scatchard plot, it was found that thyroxine-binding prealbumin only presents a single binding site for thyroxine per molecule, the association constant being 1.7 . 10(8) M-1.
D003956:Dialysis; D066298:In Vitro Techniques; D007700:Kinetics; D011228:Prealbumin / Q000378:metabolism*; D012709:Serum Albumin / Q000378:metabolism*; D013816:Thermodynamics; D013974:Thyroxine / Q000008:administration & dosage / Q000378:metabolism*; D013975:Thyroxine-Binding Proteins / Q000378:metabolism*
D011228:Prealbumin; D012709:Serum Albumin; D013975:Thyroxine-Binding Proteins; D013974:Thyroxine
Etude de la liaison thyroxine-thyroxine-binding prealbumin par une methode de dialyse a concentration libre imposee.