[Heterologous transformation in Bacillus subtilis. 1. Transmission of DNA of the R1drd19 plasmid].
Bac. subtilis 168 (BD-25) cells were infected with DNA of plasmide R1drd19 isolated from E. coli strain; transformants resistant to streptomycin (500 microgram/ml) and kanamycin (40 microgram/ml) appeared with the frequency of 2.10(-6). These transformants retained resistance to the mentioned antibiotics stably. A satellite DNA peak was revealed in centrifugation in the density gradient of cesium chloride with ethidium bromide. It was possible to infect cells of Bac. subtilis 168 (BD-25) with plasmide DNA isolated from the transformants. Plasmide transduction with the aid of phages AR9 and PBSI multiplied on the transformant strains was also effected. Physico-chemical analysis of the transformed plasmide DNA was conducted; its molecular weight was determined.
D001412:Bacillus subtilis; D046508:Culture Techniques; D004269:DNA, Bacterial; D004352:Drug Resistance, Microbial; D004926:Escherichia coli; D005111:Extrachromosomal Inheritance; D007612:Kanamycin / Q000494:pharmacology; D010957:Plasmids; D013307:Streptomycin / Q000494:pharmacology; D014170:Transformation, Genetic
D004269:DNA, Bacterial; D007612:Kanamycin; D013307:Streptomycin
Geterologichnaia transformatsiia u Bac. subtilis. Soobshchenie 1. Peredacha DNK plazmidy R1drd19.