Immunochemical investigations of human pituitary beta-lipotropin.
Antiserum to human beta-lipotropin has been raised in rabbits and characterized by gel double diffusion, quantitative precipitin, microcomplement fixation and biological neutralization tests. A sensitive radioimmunoassay for human beta-lipotropin has been developed. It was demonstrated that the antiserum shows specificity for this hormone. Human beta-melanotropin and human beta-endorphin showed only a minimal degree of cross-reaction, while ovine beta-lipotropin showed a partial but significant degree of cross-reactivity.
D003168:Complement Fixation Tests; D003429:Cross Reactions; D004723:Endorphins / Q000276:immunology; D006801:Humans; D005779:Immunodiffusion; D009074:Melanocyte-Stimulating Hormones / Q000276:immunology; D009500:Neutralization Tests; D011233:Precipitin Tests; D011863:Radioimmunoassay; D008083:beta-Lipotropin / Q000276:immunology*
D004723:Endorphins; D009074:Melanocyte-Stimulating Hormones; D008083:beta-Lipotropin