[Demonstration and characterization of mannosyl phosphoryl dolichol synthesized in the outer membrane of liver mitochondria].
There is in mitochondrial outer membrane a mannosyl-transferase which is able to catalyse the mannose transfer on a polyprenic acceptor, to give a mannosyl-phosphoryl-dolichol. This enzyme is stimulated by Mn2+, Mg2+ and dithiothreitol. It is inhibited by GDP, mersalyl and EGTA.
D000818:Animals; D004287:Dolichol Monophosphate Mannose / Q000096:biosynthesis*; D006153:Guanosine Diphosphate / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D008364:Mannosyltransferases / Q000378:metabolism; D008566:Membranes / Q000201:enzymology; D051379:Mice; D008930:Mitochondria, Liver / Q000201:enzymology*; D011105:Polyisoprenyl Phosphate Sugars / Q000096:biosynthesis*
D011105:Polyisoprenyl Phosphate Sugars; D006153:Guanosine Diphosphate; D008345:Manganese; D004287:Dolichol Monophosphate Mannose; D008364:Mannosyltransferases
Mise en évidence et caractérisation d'un mannosyl-phosphoryl-dolichol synthétisé dans la membrane externe des mitochondries de foie.