Quantitation of Hb a2 with DE-52 microchromatography in whole blood as screening test for beta-thalassemia heterozygotes.
HB A2 was assayed by means of DE-52 microchromatography in hemolysates from 285 normal subjects and 223 beta-thalassemia heterozygotes. No overlap was found between both groups. Comparable results were observed analyzing whole blood samples collected in capillary tubes from 550 normal subjects and 295 beta-thalassemia heterozygotes. Our results demonstrate that this technique is useful in a screening program for beta-thalassemia trait.
D000328:Adult; D002648:Child; D002848:Chromatography, DEAE-Cellulose / Q000379:methods*; D005260:Female; D006441:Hemoglobin A / Q000032:analysis*; D006454:Hemoglobins / Q000032:analysis*; D006579:Heterozygote; D006801:Humans; D007558:Italy; D008297:Male; D013789:Thalassemia / Q000453:epidemiology* / Q000235:genetics
D006454:Hemoglobins; D006441:Hemoglobin A