Estimation of cell damage in monolayer culture using 14C-leucine incorporation.
A comparison was made of 14C-leucine incorporation and cell viability of T24 human bladder carcinoma cells after treatment with Thio-Tepa, a potent alkylating agent. It was found that Thio-Tepa produced concentration-dependent damage in these cells and also that 14C-leucine incorporation correlated well with cell viability. 14C-leucine incorporation can be used as a reliable and rapid screening test for the assessment of the relative potencies of anticancer drugs.
D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D006801:Humans; D007930:Leucine / Q000378:metabolism*; D013852:Thiotepa / Q000494:pharmacology
D013852:Thiotepa; D007930:Leucine