[The use of primate tissue homogenates for quality control in the creatine kinase-MB determination with inhibiting antibodies (author's transl)].
The creatine kinase isoenzymes CK-MM, CK-MB and CK-BB from Rhesus monkey heart and brain tissue homogenates were separated chromatographically. Thereafter it could be demonstrated that the activity of the simian subunit CK-M is completely inhibited by anti-inhibitory-CK-M serum. Thus control sera from simian tissue are in principle suited for quality control in an immunological determination of creatine kinase-MB. The intra-assay variance and interassay variance were n = 56, -/x = 29.2 U/1, SD = 3.2 U/1, CV = 11.1% and n= 12, -/x = 166.7 U/1, SD = 5.0 U/1, CV = 3.0% respectively. It is desirable to develop control sera with catalytic concentrations of creatine kinase-MB in a lower range.
D000818:Animals; D001921:Brain / Q000201:enzymology; D003402:Creatine Kinase / Q000032:analysis* / Q000276:immunology; D000882:Haplorhini; D007527:Isoenzymes / Q000032:analysis* / Q000276:immunology; D008253:Macaca mulatta; D009206:Myocardium / Q000201:enzymology; D011786:Quality Control
D007527:Isoenzymes; D003402:Creatine Kinase
Verwendung von Primaten-Gewebshomogenaten zur Qualitätskontrolle der Bestimmung von Kreatinkinase-MB mit inhibierenden Antikörpern.