Use of argA-lac fusions to generate lambda argA-lac bacteriophages and to determine the direction of argA transcription in Escherichia coli.
Fusions of lac genes to the argA operator were constructed, and lambda phages carrying these fusions were isolated and characterized. With the aid of a lambda phage carrying an argA-lac fusion, the direction of argA transcription on the Escherichia coli chromosome was determined to be clockwise.
D001120:Arginine / Q000096:biosynthesis / Q000235:genetics*; D002876:Chromosomes, Bacterial; D003090:Coliphages / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D005814:Genes, Viral; D007785:Lactose / Q000378:metabolism; D008242:Lysogeny; D009876:Operon; D014158:Transcription, Genetic; D014161:Transduction, Genetic; D001616:beta-Galactosidase / Q000096:biosynthesis
D001120:Arginine; D001616:beta-Galactosidase; D007785:Lactose