HLA-D typing of human lymphocytes using frozen and thawed spermatozoa.
A method is described for the preservation of haploid populations of human spermatozoa in liquid nitrogen for their subsequent use as stimulators in HLA-D (SL) lymphocyte typing. The typing results obtained using thawed spermatozoa correlated well with those obtained when using fresh sperm and also with other cellular typing methods.
D005615:Freezing; D006238:Haploidy; D006650:Histocompatibility Testing; D006801:Humans; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D013094:Spermatozoa / Q000276:immunology*