title: Response of specific transfer-ribonucleic-acid levels to amino-acid deprivation in Friend leukemia cells.
abstract: In eukaryotes, the levels of specific tRNAs are closely correlated with the demands for their cognate amino acids in protein synthesis. To account for this phenomenon, we have proposed that the extent of aminoacylation of a given tRNA species in vivo controls the relative rate of synthesis or turnover of that species. Previously, we reported that Friend leukemia cells respond to histidine deprivation by increasing their relative level of tRNAHis by as much as two-fold, with no change in the relative level of tRNALeu. In this paper, we show that deprivation of leucine or tryptophan also causes a specific increase in the relative level of tRNAs cognate to the deprived amino acid. At least in the case of tRNATrp, the increases in relative tRNA levels are preceded by extensive declines in the steady-state extent of aminoacylation of the tRNA in vitro. We also find that different isoacceptors may respond differently to amino acid deprivation. These results suggest that decreased extents of aminoacylation of a given tRNA species in vivo cause increases in the relative rate of synthesis or decreases in the relative rate of degradation of that species.
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D007942:Leukemia, Experimental / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000096:biosynthesis; D014176:Protein Biosynthesis; D012343:RNA, Transfer / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D009363:Neoplasm Proteins; D012343:RNA, Transfer