Affinity chromatography of the cellulase system of Trichoderma koningii.
A procedure involving affinity chromatography on cellulose was developed for separating enzymic components of the cellulase complex. Cellobiase, carboxymethylcellulase, component C2 and cellobiohydrolase are adsorbed with increasing tenacity, and released, as highly purified components, as the ionic strength of the eluent is decreased.
D002480:Cellulase / Q000302:isolation & purification*; D002482:Cellulose; D002846:Chromatography, Affinity; D003904:Mitosporic Fungi / Q000201:enzymology*; D009097:Multienzyme Complexes / Q000302:isolation & purification; D014242:Trichoderma / Q000201:enzymology*; D001617:beta-Glucosidase / Q000302:isolation & purification
D009097:Multienzyme Complexes; D002482:Cellulose; D001617:beta-Glucosidase; D002480:Cellulase