[Effect of time in culture on the stimulatory effect of 17-beta estradiol on adenohypophyseal hormone release (author's transl)].
Secretion of LH, FSH, TSH and prolactin was studied in rat anterior pituitary cells in primary culture. Basal LH release, LH cell content and LH responsiveness to LHRH decreased with a half-life of 4 days. Basal FSH decreased only 20% up to 20 days thus indicating the relative independence of FSH secretion compared to that of LH. The stimulatory effect of 17-beta estradiol on LH and FSH secretion was higher during the first days in culture. The stimulatory effect of 17-beta estradiol on prolactin secretion did however remain maximal up to 20 days.
D000818:Animals; D002478:Cells, Cultured; D004958:Estradiol / Q000494:pharmacology*; D005640:Follicle Stimulating Hormone / Q000378:metabolism; D066298:In Vitro Techniques; D007986:Luteinizing Hormone / Q000378:metabolism; D010903:Pituitary Gland, Anterior / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D010908:Pituitary Hormones, Anterior / Q000378:metabolism*; D011388:Prolactin / Q000378:metabolism; D051381:Rats; D013268:Stimulation, Chemical; D013973:Thyrotropin-Releasing Hormone / Q000378:metabolism; D013997:Time Factors
D010908:Pituitary Hormones, Anterior; D004958:Estradiol; D013973:Thyrotropin-Releasing Hormone; D011388:Prolactin; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone
Effet du temps en culture sur la sensibilité de la réponse des cellules adénohypophysaires à l'oestradiol-17 beta.