title: An actin-like protein is a component of axonemes from Chlamydomonas flagella.
abstract: Several lines of evidence indicate that a component of axonemes from Chlamydomonas flagella is similar to actin from rabbit skeletal muscle. The polypeptide has an apparent molecular weight of 42,000 and in a pH gradient has the electrophoretic behavior of beta-actin. It was co-polymerized with rabbit actin and purified by affinity chromatography on DNase I-Sepharose. Incomplete proteolysis of mixed 35S-labeled axonemal protein and cold rabbit actin formed similar sets of peptides as analyzed by one-dimensional gel electrophoresis. The actin-like protein and the tubulin appear to be present in the axoneme in the molar ratio 1:60.
mesh_terms: D000199:Actins / Q000032:analysis*; D000818:Animals; D002696:Chlamydomonas / Q000032:analysis*; D005407:Flagella / Q000032:analysis*; D008970:Molecular Weight; D009132:Muscles / Q000032:analysis; D011487:Protein Conformation; D011817:Rabbits; D014404:Tubulin
chemical_list: D000199:Actins; D014404:Tubulin