title: Effect of intraocular irrigating solutions on intracellular membrane potentials and swelling rate of isolated human and rabbit cornea.
abstract: Isolated human and rabbit corneas were incubated in glutathione bicarbonate Ringer solution (GBR), balanced salt solution (BSS), or 0.9% NaCl solution. The swelling rate of human corneas was 25.5 micron/hr in GBR and significantly increased to 32.7 in BSS and 66.1 in NaCl. The epithelial intracellular potential of human cornea was constant at about 60 mV up to 5 hr of incubation in GBR and decreased continuously to 40 mV in BSS and NaCl. Endothelial cell potentials were stable for up to 3 hr of incubation in GBR or BSS and decreased from a control value of about 18 mV to 10 mV 2 hr after bathing in NaCl. Qualitatively similar data were obtained in the isolated rabbit cornea. The results demonstrate the advantage of GBR as an intraocular irrigating solution.
mesh_terms: D000818:Animals; D003315:Cornea / Q000187:drug effects* / Q000473:pathology / Q000502:physiology; D005978:Glutathione / Q000009:adverse effects / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D008564:Membrane Potentials / Q000187:drug effects*; D011817:Rabbits; D012965:Sodium Chloride / Q000009:adverse effects / Q000494:pharmacology*; D012996:Solutions; D007507:Therapeutic Irrigation / Q000009:adverse effects
chemical_list: D012996:Solutions; D012965:Sodium Chloride; D005978:Glutathione