Photosynthetic activity of diimidoester-modified cells, permeaplasts, and cell-free membrane fragments of the blue-green alga Anacystis nidulans.
On treating the blue green alga Anacystis nidulans with dimethylsuberimidate up to 70% of the free NH2 of the photosynthetic membrane is amidinated, and presumably inter- and intramolecular cross-links are established in the membrane proteins. Amidination destroys the ability of A. nidulans to photoreduce HCO3(-) but leaves the photochemical activities of Photosystems II and I nearly intact. With added electron acceptors, photosynthetic O2 evolution can be demonstrated both with permeable cells (permeaplasts) prepared by digestion of the cell wall of dimethylsuberimidate-reacted A. nidulans with lysozyme, as well as with heavy membrane particles (36 000 x g) prepared from dimethylsuberimidate-reacted cells. Permeaplasts prepared from dimethylsuberimidate-reacted cells resist damage in hypoosmotic medium, whereas those prepared from unreacted cells are induced to release C-phycocyanin. On the other hand, the former are inactivated more easily by heat stress than the latter. On this basis, it is concluded that cross-linking with dimethylsuberimidate confers functional instability to photosynthetic membranes.
D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D002474:Cell-Free System; D055598:Chemical Phenomena; D002621:Chemistry; D000458:Cyanobacteria / Q000187:drug effects*; D004120:Dimethyl Suberimidate / Q000494:pharmacology*; D004579:Electron Transport / Q000187:drug effects; D005404:Fixatives; D006358:Hot Temperature; D007094:Imides / Q000494:pharmacology*; D008565:Membrane Proteins; D009113:Muramidase / Q000378:metabolism; D009994:Osmolar Concentration; D010788:Photosynthesis / Q000187:drug effects*; D010798:Phycocyanin / Q000378:metabolism; D013057:Spectrum Analysis
D005404:Fixatives; D007094:Imides; D008565:Membrane Proteins; D010798:Phycocyanin; D004120:Dimethyl Suberimidate; D009113:Muramidase