Short term in vitro cultivation of malaria parasites in Papua New Guinea for preparation of biosynthetically-labelled parasite antigens.
Short term in vitro cultivation has been used successfully for biosynthetic labelling of TCA precipitable macromolecules of Papua New guinean isolates of Plasmodium falciparum. Conditions satisfactory for completion of the asexual erythrocytic cycle were achieved with aa minimum of sophisticated equipment. The techniques used are described in detail and mention is made of possible applications to assessment of chloroquine resistance and the mode of action of inhibitory antisera.
D000941:Antigens / Q000276:immunology*; D003470:Culture Media; D006801:Humans; D066298:In Vitro Techniques; D008288:Malaria / Q000469:parasitology; D008828:Microbiological Techniques; D010963:Plasmodium falciparum / Q000254:growth & development* / Q000276:immunology; D013997:Time Factors; D014316:Tritium
D000941:Antigens; D003470:Culture Media; D014316:Tritium