Determination of kallidinogenase activity by radial immunodiffusion methods.
An attempt has been made to elaborate a method allowing determination of kallidinogenase obtained from the pancreas of slaughtered animals (pigs), by means of radial immunodiffusion method. As the test substance (antigen) a pharmaceutical preparation Dilminal D has been used. The immune serum against the preparation administered was obtained from rabbits. This serum was used to saturate plates of agar gel. Little pigs cut in the gel were filled with various concentrations of the preparation (antigen). A linear correlation between the selected concentrations of the preparation was obtained. This method may find application in determining preparations of kallidinogenase (from pig pancreas) with titer value given in different units.
D000818:Animals; D005779:Immunodiffusion; D007610:Kallikreins / Q000378:metabolism*; D010179:Pancreas / Q000201:enzymology*; D013552:Swine
D007610:Kallikreins