[Validity of quantitative protein A determination of "Staphylococcus aureus" by radial immunodiffusion].
The validity of quantitative determination of protein A by radial immunodiffusion has been tested using human serum, human IgG and porcine serum. The best results are obtained with porcine serum. Maximal sensitivity is obtained after 48 h of incubation with 15 microliters of porcine serum per 13 ml of gel. For these particular conditions the range of linearity varies between 20 and 300 microgram/ml.
D000818:Animals; D005069:Evaluation Studies as Topic; D006801:Humans; D007106:Immune Sera; D005779:Immunodiffusion / Q000379:methods; D007074:Immunoglobulin G; D013205:Staphylococcal Protein A / Q000032:analysis*; D013211:Staphylococcus aureus / Q000032:analysis*; D013552:Swine / Q000276:immunology
D007106:Immune Sera; D007074:Immunoglobulin G; D013205:Staphylococcal Protein A
Valeur de l'immunodiffusion radiale pour le dosage de la protéine A de Staphylococcus aureus.