Dose-effects of 8-methoxypsoralen and long wave UV-light in 3T3 cells: evaluation of phototoxic index.
3T3 cells were cultured until confluency and treated with various doses of 8-methoxypsoralen followed by long wave UV light irradiation. The inhibition of 3H-thymidine incorporation was dose-dependent for both, psoralen and light. A phototoxic index (PTI) was established demonstrating that a constant correlation between psoralen and UVA light exists for the photoinactivation in living cells.
D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects* / Q000528:radiation effects; D004247:DNA / Q000096:biosynthesis*; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D008730:Methoxsalen / Q000494:pharmacology*; D051379:Mice; D010778:Photochemotherapy; D014466:Ultraviolet Rays
D004247:DNA; D008730:Methoxsalen