Evidence for a probenecid-sensitive transport system of acid monoamine metabolites from the spinal subarachnoid space.
The location of probenecid-sensitive elimination mechanisms of monoamine metabolites from the cerebrospinal fluid of the cat was determined with ventriculo-cisternal or ventriculo-lumbar perfusion techniques. These techniques were described in detail. Levels of endogenous homovanillic acid and 5-hydroxyindoleacetic acid were assayed in the perfusate. Probenecid administration induced the most marked increase of the levels of the monoamine metabolites in the ventriculo-lumbar perfusates. It was concluded that probenecid blocked the transport of both metabolites from the spinal subarachnoid space.
D000818:Animals; D001679:Biogenic Amines / Q000378:metabolism*; D001693:Biological Transport, Active / Q000187:drug effects; D002415:Cats; D005260:Female; D006719:Homovanillic Acid / Q000134:cerebrospinal fluid / Q000378:metabolism; D006897:Hydroxyindoleacetic Acid / Q000134:cerebrospinal fluid / Q000378:metabolism; D008297:Male; D010477:Perfusion; D011339:Probenecid / Q000494:pharmacology*; D013116:Spinal Cord / Q000187:drug effects / Q000378:metabolism; D013346:Subarachnoid Space / Q000187:drug effects / Q000378:metabolism*; D013997:Time Factors
D001679:Biogenic Amines; D006897:Hydroxyindoleacetic Acid; D011339:Probenecid; D006719:Homovanillic Acid