[Demonstration of zinc-metalloenzyme syntheses after zinc supplements for deficient animals by means of measurement of the incorporation of 65Zn in various organs].
For the purpose of detecting the synthesis of zinc metalloenzymes after zinc supplementation in an experiment using rats the animals were first depleted for 15 days and subsequently injected a labelled zinc salt solution (65Zn-ZnCl2) in a dosis of 0.4 mg (in terms of Zn) and with an activity of 3.0 muCi/100microliter. After a 5-day depletion period, the activity of the metallo-enzymes alkaline phosphatase in plasma and in the femur and carboxy-peptidase A and B in the pancreatic gland was found to rise at the same rate as the 65Zn-measuring rate in plasma, femur and pancreatic gland. By calculating correlations this interdependence was demonstrated. Thus the highly significant correlation coefficients prove for these metalloenzymes that a synthesis with the injected zinc salt has taken place whilst for carbo-anhydrase in blood this evidence was not furnished. As the zinc dosis is not exclusively used for the enzyme synthesis, but additional 65Zn is incorporated into the individual organs, it does not appear to be possible to draw conclusions from the 65 Zn-measuring rate in the individual organs on the intermediary availability.
D000469:Alkaline Phosphatase / Q000378:metabolism; D000821:Animal Feed; D000818:Animals; D002256:Carbonic Anhydrases / Q000378:metabolism; D002268:Carboxypeptidases / Q000378:metabolism; D004790:Enzyme Induction; D004912:Erythrocytes / Q000201:enzymology; D005269:Femur / Q000201:enzymology; D005523:Food Supply; D008297:Male; D010179:Pancreas / Q000201:enzymology; D051381:Rats; D015032:Zinc / Q000172:deficiency*
D000469:Alkaline Phosphatase; D002268:Carboxypeptidases; D002256:Carbonic Anhydrases; D015032:Zinc
Zum Nachweis von Zink-Metalloenzym-Synthesen nach Zinkzulagen an Mangeltiere durch Messung der Inkorporierung von 65Zn in einzelnen Organen.