[Secretion of insulin and of glucagon by the perfused pancreas of newborn rats: effect of vasoactive intestinal peptide Vip].
A method is described for perifusion of the splenic part of the pancreas from 48-64 hour-old rat. In different basal conditions, the secretion of insulin and glucagon is stable and reproducible for 90 mn. The addition of the vasoactive intestinal peptide (VIP) to these perifusion media, at a concentration as low as 2 ng/ml, determines a remarkable increase of insulin and of glucagon secretion. These results suggest the possibility of a VIP action in the physiology of endocrine pancreas.
D000596:Amino Acids / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn / Q000378:metabolism*; D005768:Gastrointestinal Hormones / Q000494:pharmacology*; D005934:Glucagon / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology; D066298:In Vitro Techniques; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism / Q000494:pharmacology*
D000596:Amino Acids; D005768:Gastrointestinal Hormones; D007328:Insulin; D014660:Vasoactive Intestinal Peptide; D005934:Glucagon; D005947:Glucose
Sécrétion d'insuline et de glucagon par le pancréas perifusé de rat nouveau-né: effet du peptide intestinal vasoactif ou VIP.