title: An immunological approach to the isolation of factors with mitotic activity from the plasmodial stage of the myxomycete Physarum polycephalum.
abstract: Nuclear divisions in plasmodia of Physarum polycephalum were advanced by applying immunologically purified plasmodial extracts of late G2 phase on the surface of plasmodia which were 1.5 h before the third mitosis. The purification of G2 extracts was achieved by interaction of antibodies prepared against the antigens of early S phase plasmodia with the antigens of late G2 plasmodia. There was no advancement of mitosis by extracts prepared from early S phase plasmodia. Untreated G2 extracts did not accelerate mitosis with the same effectiveness as did antibody purified G2 extracts.
mesh_terms: D007158:Immunologic Techniques; D007399:Interphase; D008938:Mitosis; D010804:Physarum / Q000032:analysis* / Q000166:cytology