Stepwise selection of defective nitrogen-fixing phenotypes in Escherichia coli K-12 by dimethyl sulfoxide.
Storage in dimethyl sulfoxide of Escherichia coli K-12 hybrids carrying Klebsiella pneumoniae nif+ genes can result in selection of a defective nitrogen-fixing phenotype. Dimethyl sulfoxide appears to affect particular inner membrane proteins associated with energy metabolism in E. coli K-12.
D003470:Culture Media; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004734:Energy Metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005798:Genes, Bacterial; D006824:Hybridization, Genetic; D009586:Nitrogen Fixation / Q000187:drug effects*; D010641:Phenotype
D003470:Culture Media; D004121:Dimethyl Sulfoxide