title: Induced dominant lethal mutations and cytotoxic effects in germ cells of Drosophila melanogaster with Trenimon, PDMT and sodium monofluorophosphate.
abstract: Male and female Drosophila melanogaster with special sex chromosome or special autosome constitutions were fed with the mutagenic chemicals Trenimon (2,3,5-trisethyleneimino-1,4-benzoquinone) and PDMT (1-phenyl-3, 3-dimethyltriazene) and with the toxic substance Na2PO3F (sodium monofluorophosphate). The frequency of dominant lethality was recorded among the progeny. The results clearly show that dominant lethality is dose dependent for Trenimon- or PDMT-treated chromosomes in mature sperm and mature oocytes, and an increased amount of chromosomal material per nucleus yields an enhanced lethality. In contrast, a pure toxic effect of Na2PO3F on mature oocytes was demonstrated with one type of female. --With the stocks of Drosophila used, it is possible to distinguish between mutagenic and toxic effects of chemicals on the germ cells. Therefore, dominant lethality can be used as a simple and quick screening test for chemical mutagens.
mesh_terms: D000818:Animals; D002875:Chromosomes / Q000187:drug effects; D003433:Crosses, Genetic; D004305:Dose-Response Relationship, Drug; D004331:Drosophila melanogaster / Q000187:drug effects / Q000235:genetics; D005260:Female; D005459:Fluorides / Q000494:pharmacology*; D005799:Genes, Dominant; D005804:Genes, Lethal; D008297:Male; D009153:Mutagens / Q000494:pharmacology; D009154:Mutation; D009865:Oocytes / Q000187:drug effects; D010710:Phosphates / Q000494:pharmacology*; D013094:Spermatozoa / Q000187:drug effects; D014226:Triazenes / Q000494:pharmacology*; D014228:Triaziquone / Q000494:pharmacology*
chemical_list: D009153:Mutagens; D010710:Phosphates; D014226:Triazenes; C012980:fluorophosphate; D014228:Triaziquone; D005459:Fluorides