Effect of prostaglandins on steroidogenesis by bovine adrenal cortex mitochondria.
Prostaglandins (PGA1, PGE2, PGF2 alpha) were found to increase cholesterol side-chain clevage activity in isolated bovine adrenal cortex mitochondria, provided calcium was present in the incubation medium. Optimal stimulation was observed at low PG concentrations (10-7 to 10-9 M), with malate or malate-NADPH supported side-chain cleavage. Under the same conditions, two endoperoxide analogs and several fatty acids were ineffective. The PG action was not observed with a mitochondrial acetone powder preparation. These observations suggest that primary PG may act by interfering with calcium distribution at the mitochondrial level, leading to the activation of cholesterol side-chain cleavage. Thus, an intracellular action of endogenous PG may be considered in the regulation of adrenal cortex steroidogenic functions.
D000302:Adrenal Cortex / Q000187:drug effects / Q000378:metabolism*; D000311:Adrenal Glands / Q000378:metabolism*; D000818:Animals; D002118:Calcium / Q000494:pharmacology; D002417:Cattle; D002784:Cholesterol / Q000378:metabolism*; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000378:metabolism; D004492:Edetic Acid / Q000494:pharmacology; D005230:Fatty Acids, Nonesterified / Q000494:pharmacology; D007700:Kinetics; D008293:Malates / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D011284:Pregnenolone / Q000378:metabolism*; D011453:Prostaglandins / Q000494:pharmacology*; D011454:Prostaglandins A / Q000494:pharmacology*; D011458:Prostaglandins E / Q000494:pharmacology*; D011460:Prostaglandins F / Q000494:pharmacology*
D005230:Fatty Acids, Nonesterified; D008293:Malates; D011453:Prostaglandins; D011454:Prostaglandins A; D011458:Prostaglandins E; D011460:Prostaglandins F; D011284:Pregnenolone; D002784:Cholesterol; D004492:Edetic Acid; D002786:Cholesterol Side-Chain Cleavage Enzyme; D002118:Calcium