title: Coenzyme M and methylcobalamin in methane biosynthesis: results of model studies.
abstract: Coenzyme M (2-mercaptoethanesulfonate, HS-CH2CH2SO3-) reacts with methylcobalamin nonenzymatically in the pH-range between 6 and 14 to yield the S-methyl derivative (CH3-S-CH2CH2SO3-). In addition, and also at lower pH, methane is produced by reductive cleavage of the Co-C bond. With methylcobaloximes as the methyl group donors, methane production predominates, with insignificant S-methylation. The initial rates of methane production from methylcobaloximes with coenzyme M as the reductant correlate with the rates of methane production from these substrates with active cell extracts of Methanobacterium M.o.H.
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D008623:Mercaptoethanol / Q000031:analogs & derivatives*; D015080:Mesna / Q000378:metabolism*; D008697:Methane / Q000096:biosynthesis*; D008745:Methylation; D010084:Oxidation-Reduction; D010091:Oximes / Q000378:metabolism; D013329:Structure-Activity Relationship; D014805:Vitamin B 12 / Q000378:metabolism* / Q000031:analogs & derivatives
chemical_list: D010091:Oximes; D008623:Mercaptoethanol; C019476:mecobalamin; D015080:Mesna; D008697:Methane; D014805:Vitamin B 12