Glycosylation of endogenous lipids and proteins by preparations of chicken embryo fibroblasts.
Glycosylation of endogenous phosphoisoprenyl lipids and membrane-associated proteins was shown to occur in preparations of chicken embryo fibroblasts incubated with GDP[14C]mannose and UDP-N-acetylglucosamine. The two preparations used were cells released from the culture dishes by buffered saline containing EDTA and crude membranes from those cells. Both beta-mannosyl-phosphoryldolichol and oligosaccharide-phosphoryl lipids with five to eight sugar residues were labelled under the conditons employed. The oligosaccharide isolated from the octasaccharide-lipid fraction was shown to be heterogeneous after an analysis of the products formed by treatment of the oligosaccharide with glycosidases. Some of the oligosaccharides appeared to contain N-acetylglucosamine at positions external to that of [14C]mannose. Lipids with oligosaccharide moieties of different structures were made by the two preparations. The results of pulse-chase experiments were consistent with the glycosylated lipids being intermediates in glycoprotein biosynthesis.
D000118:Acetylglucosaminidase; D000818:Animals; D002642:Chick Embryo; D002845:Chromatography; D004492:Edetic Acid; D005347:Fibroblasts / Q000378:metabolism*; D006155:Guanosine Diphosphate Mannose / Q000378:metabolism*; D050356:Lipid Metabolism; D008358:Mannose / Q000378:metabolism; D008361:Mannosidases; D009702:Nucleoside Diphosphate Sugars / Q000378:metabolism*; D009844:Oligosaccharides; D011506:Proteins / Q000378:metabolism*; D012995:Solubility; D014537:Uridine Diphosphate N-Acetylglucosamine / Q000378:metabolism
D009702:Nucleoside Diphosphate Sugars; D009844:Oligosaccharides; D011506:Proteins; D006155:Guanosine Diphosphate Mannose; D014537:Uridine Diphosphate N-Acetylglucosamine; D004492:Edetic Acid; D008361:Mannosidases; D000118:Acetylglucosaminidase; D008358:Mannose