Improvement in the resolution of human sperm protamines by use of iodoacetamide as alkylating agent.
By use of the neutral alkylating agent iodo [14C1] acetamide instead of ethylene imine or iodoacetate, the resolution of human protamines on gel electrophoresis and ion-exchange chromatography has been improved. Using 20-cm gels, human protamines may be fractionated into seven bands, including the two chromatographically distinct forms of HP1 and a hitherto undetected component HP4. On ion-exchange chromatography, HP2 and the two forms of HP1 may be isolated in sufficient purity for sequence analysis.
D002852:Chromatography, Ion Exchange; D006801:Humans; D007460:Iodoacetamide; D007461:Iodoacetates; D008297:Male; D008970:Molecular Weight; D011479:Protamines / Q000302:isolation & purification*; D013094:Spermatozoa / Q000032:analysis*
D007461:Iodoacetates; D011479:Protamines; D007460:Iodoacetamide