A study of folic-acid-binding protein in normal subjects.
6 normal females were found to have a level of folic-acid-binding protein (FABP) in their sera (mean level 345 pg/ml). Lysates of their various leucocytes were assayed and FABP found exclusively in the polymorph fraction. The sera and lysates were fractionated by Sephadex G-200 gel column chromatography and the molecular weight of the FABP appeared to be approximately 40,000. A hitherto unreported inhibitory effect of heparin on folate binding was noticed. Studies have been performed on folic-acid-binding protein (FABP) in various pathological states, but the levels found in normal people are usually considered too low to permit investigation. In an earlier study [3], 6 normal females were found to have levels in excess of 300 pg/ml whilst the mean level of 94 controls was 76 pg/ml. Sera and leucocyte lysates from these people have been  used to estimate the molecular weight of FABP in normal people.
D000328:Adult; D000368:Aged; D000818:Animals; D001798:Blood Proteins / Q000032:analysis; D002850:Chromatography, Gel; D005260:Female; D005492:Folic Acid / Q000097:blood*; D006493:Heparin / Q000494:pharmacology; D006801:Humans; D007962:Leukocytes / Q000032:analysis; D008297:Male; D008875:Middle Aged; D008895:Milk, Human / Q000032:analysis; D008970:Molecular Weight; D011485:Protein Binding / Q000187:drug effects; D013552:Swine
D001798:Blood Proteins; D006493:Heparin; D005492:Folic Acid