The effects of IgG2 and of antigen concentration on prozoning in the complement fixation test for bovine brucellosis.
Addition of Brucella-specific IgG2 to solutions of Brucella-specific IgG1 initially induced prozoning and at higher concentrations prevented all reaction in the complement fixation test (CFT) for bovine brucellosis. Some infected cattle may be diagnosed as brucellosis-free due to a high ratio of specific IgG2 to IgG1. Increasing the concentration of antigen in the CFT reduced the tendency to prozone.
D000818:Animals; D000942:Antigens, Bacterial; D002003:Brucella abortus; D002007:Brucellosis, Bovine / Q000175:diagnosis*; D002417:Cattle; D003168:Complement Fixation Tests; D005260:Female; D007074:Immunoglobulin G / Q000032:analysis*
D000942:Antigens, Bacterial; D007074:Immunoglobulin G