title: Interaction of ethidium bromide with ribosomes. Absorption, fluorescence, circular dichroism and sedimentation studies.
abstract: It is shown in this work that the binding of ethidium bromide to yeast ribosomes occurs through intercalation in the double-stranded rRNA regions and produces changes in the ribosomes structure, yielding unfolded subparticles, and even partial separation of proteins from rRNA at very high binding ratios. The addition of Mg2+ prevents these structural changes, probably by partial inhibition of the dye binding.
mesh_terms: D001665:Binding Sites; D002942:Circular Dichroism; D004492:Edetic Acid / Q000494:pharmacology; D004996:Ethidium / Q000378:metabolism*; D008274:Magnesium / Q000494:pharmacology; D012335:RNA, Ribosomal / Q000378:metabolism; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism*; D012441:Saccharomyces cerevisiae; D013050:Spectrometry, Fluorescence; D014461:Ultracentrifugation
chemical_list: D012335:RNA, Ribosomal; D004492:Edetic Acid; D004996:Ethidium; D008274:Magnesium