Isolation and characterization of the glycoprotein (JBB5) in the urine of a patient with carcinoma of the colon.
Patient J. B. with metastatic carcinoma of the colon excreted 0.5 to 1.0 g protein daily, about one-third of which was in Molecular Weight Class 30,000 to 60,000. The major component of this class was isolated by gel filtration and ion-exchange chromatography. The purified protein, labeled JBB5, contained about 11% sialic acid, 8% hexose, and 4% hexosamine. Its molecular weight was between 51,000 and 59,000. It did not react detectably with antisera to any of the recognized normal human plasma proteins. A specific antiserum to JBB5 was raised in the rabbit. Urine from 4% of subjects with nonneoplastic illnesses reacted in double immunodiffusion with anti-JBB5. Thirty-three % positive reactions were obtained with urines from patients with advanced neoplastic disease, the percentage varying from 64% in metastatic cancer of the pancreas to 15% in chronic lymphocytic leukemia.
D000230:Adenocarcinoma / Q000276:immunology / Q000652:urine*; D000328:Adult; D000912:Antibodies, Neoplasm; D003110:Colonic Neoplasms / Q000276:immunology / Q000652:urine*; D003429:Cross Reactions; D005260:Female; D006023:Glycoproteins / Q000276:immunology / Q000302:isolation & purification / Q000652:urine*; D006801:Humans; D005779:Immunodiffusion; D007938:Leukemia / Q000652:urine; D007945:Leukemia, Lymphoid / Q000276:immunology; D008970:Molecular Weight; D009362:Neoplasm Metastasis; D009363:Neoplasm Proteins / Q000276:immunology / Q000302:isolation & purification / Q000652:urine*; D009369:Neoplasms / Q000276:immunology / Q000652:urine; D011247:Pregnancy
D000912:Antibodies, Neoplasm; D006023:Glycoproteins; D009363:Neoplasm Proteins