title: Biochemical studies of isolated rat hepatocytes from normal and phenobarbital-treated liver as obtained by rate zonal centrifugation.
abstract: Cell suspensions from normal and phenobarbital treated livers were analysed by rate zonal centrifugation in ficoll gradients. No difference in the microsomal cytochrome P-450 and NADPH-cytochrome C reductase content and in the incorporation rate of radioactive leucine was noted between light and heavy hepatocytes. After centrifugation of a mixture of radioactive phenobarbital-treated cells and normal liver cells, the specific radioactivity was constant, indicating that the hepatocytes from drug-treated liver had the same density as the normal cells.
mesh_terms: D000818:Animals; D002498:Centrifugation; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D007930:Leucine / Q000378:metabolism; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D010634:Phenobarbital / Q000494:pharmacology*; D051381:Rats
chemical_list: D003577:Cytochrome P-450 Enzyme System; D009251:NADPH-Ferrihemoprotein Reductase; D007930:Leucine; D010634:Phenobarbital