Characterization of a lysine-specific active transport system in Rickettsia prowazeki.
Rickettsia prowazeki possesses an active transport system for lysine with a Kt of influx of 1 muM. Extraction and chromatographic analysis of the accumulated labeled material show the material to be lysine rather than a derivative. This intracellular lysine pool can be exchanged with external unlabeled substrates for at least 10 min; The lysine analogues L-aminoethyl cysteine, N-methyl lysine, hydroxylysine, and D-lysine competitively inhibit uptake of L-lysine, but cadaverine, diaminopimelate, arginine, ornithine, and epsilon-aminocaproate do not. Accumulation of lysine can be inhibited by the energy poisons potassium cyanide, triphenylmethyl phosphonium bromide, and 2,4-dinitrophenol. The effect of potassium cyanide, but not 2,4-dinitrophenol or triphenylmethyl phosphonium bromide, can be overcome by adenosine 5'-triphosphate. Both energy-dependent influx and energy-independent efflux are inhibited by the sulfhydryl reagents N-ethyl maleimide and p-chloromercuriphenyl sulfonic acid.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D001667:Binding, Competitive; D001693:Biological Transport, Active / Q000187:drug effects; D002729:Chloromercuribenzoates / Q000494:pharmacology; D003486:Cyanides / Q000494:pharmacology; D004140:Dinitrophenols / Q000494:pharmacology; D005033:Ethylmaleimide / Q000494:pharmacology; D007700:Kinetics; D008239:Lysine / Q000378:metabolism*; D012283:Rickettsia prowazekii / Q000378:metabolism*; D013237:Stereoisomerism; D013696:Temperature; D014320:Triphenylmethyl Compounds / Q000494:pharmacology
D002729:Chloromercuribenzoates; D003486:Cyanides; D004140:Dinitrophenols; D014320:Triphenylmethyl Compounds; D000255:Adenosine Triphosphate; D008239:Lysine; D005033:Ethylmaleimide