Effect of phalloidin on actin proteolysis as measured by viscometry and fluorimetry.
Even at low concentration, phalloidin shows a marked protection of F-actin against the action of trypsin or pronase. G-actin is not protected at any concentration of phallodin. The kinetics of the proteolysis show that a change in the environment of tryptophan residues is preceded by disruption of the filamentous structure of F-actin.
D000199:Actins; D000818:Animals; D007700:Kinetics; D009132:Muscles; D009842:Oligopeptides / Q000494:pharmacology*; D010590:Phalloidine / Q000494:pharmacology*; D011402:Pronase / Q000037:antagonists & inhibitors*; D011817:Rabbits; D013050:Spectrometry, Fluorescence; D014361:Trypsin Inhibitors; D014783:Viscosity
D000199:Actins; D009842:Oligopeptides; D014361:Trypsin Inhibitors; D010590:Phalloidine; D011402:Pronase