In vitro effect of an acute nonspecific inflammatory exudate on tritiated-thymidine incorporation by unstimulated and PHA-stimulated spleen cells.
The effect of an acute nonspecific inflammatory exudate with mitogenic activity for macrophages in culture has been tested on the spontaneous and PHA-induced DNA synthesis by spleen cells in vitro. Stimulatory effect of this exudate was observed on the spontaneous DNA synthesis which was detectable over a range of 1:4 to 1:4096 concentrations. After optimal PHA stimulation, an inhibition of mitogen-induced DNA synthesis was observed when the cells were exposed to the highest concentrations (up to 1:128) of the exudate. Thereafter, the phenomenon could be reversed and the stimulation was maximal at the concentration of 1:2048. When a suboptimal dose of PHA was used, the stimulatory effect was more pronounced and detected from 1:8 up to 1:4096 concentrations.
D000818:Animals; D004247:DNA / Q000096:biosynthesis; D005122:Exudates and Transudates / Q000276:immunology*; D007249:Inflammation / Q000276:immunology*; D010835:Phytohemagglutinins / Q000494:pharmacology; D051381:Rats; D011919:Rats, Inbred Strains; D013154:Spleen / Q000166:cytology / Q000378:metabolism*; D013936:Thymidine / Q000378:metabolism*
D010835:Phytohemagglutinins; D004247:DNA; D013936:Thymidine