Purification and properties of the phenprocoumon-induced decarboxyfactor X from bovine plasma. A comparison to normal factor X.
1. By a procedure involving adsorption to barium sulfate, chromatography on DEAE-Sephadex and QAE-Sephadex and preparative polyacrylamide gel electrophoresis, decarboxyfactor X was purified from plasma of phenprocoumon-treated cows. No contaminants could be detected in the final preparation by polyacrylamide gel electrophoresis and zone-electrophoresis. 2. The molecular weight of decarboxyfactor X, as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis is approximately 55 000, which is equal to that of factor X. The protein consists of two polypeptide chains with molecular weights of 44 000 and 17 000. 3. Decarboxyfactor X has antigenic determinants in common with normal factor X. 4. The amino acid composition and aminoterminal amino acids of normal factor X and decarboxyfactor X are identical. 5. Less than one residue of gamma-carboxyglutamate could be detected per mole of decarboxyfactor X. 6. In the absence of Ca2+, normal factor X has a slightly higher electrophoretic mobility than decarboxyfactor X. In the presence of Ca2+ the mobility of factor X decreases considerably while the mobility of decarboxyfactor X remains unaltered.
D015110:4-Hydroxycoumarins / Q000494:pharmacology*; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002118:Calcium; D002417:Cattle; D003653:Decarboxylation; D005170:Factor X / Q000302:isolation & purification; D005260:Female; D005779:Immunodiffusion; D007123:Immunoelectrophoresis, Two-Dimensional; D046911:Macromolecular Substances; D008970:Molecular Weight; D010644:Phenprocoumon / Q000494:pharmacology*
D015110:4-Hydroxycoumarins; D000596:Amino Acids; D046911:Macromolecular Substances; D005170:Factor X; D010644:Phenprocoumon; D002118:Calcium