title: A selected ion monitoring assay for primaquine in plasma and urine.
abstract: The antimalarial drug primaquine was analysed in plasma and urine by gas chromatography mass spectrometry, using a deuterated internal standard. After freeze-drying and extraction with trichloroethylene the sample plus internal standard was reacted with Tri Sil TBT (a 3:3:2 by volume mixture of trimethylsilylimidazole, N,O,-bis-(trimethylsilylacetamide and trimethylchlorosilane) and an aliquot injected into the gas chromatograph mass spectrometer. The gas chromatographic effluent was monitored at m/z 403 and m/z 406, the molecular ions of the bis-TMS ethers of primaquine and 6-trideuteromethoxy primaquine. Calibration curves were prepared from standards made up in plasma and urine. Data from the analysis of plasma and urine samples from a volunteer who ingested the equivalent of 45 mg primaquine are presented.
mesh_terms: D000328:Adult; D002849:Chromatography, Gas; D006801:Humans; D007477:Ions; D008297:Male; D013058:Mass Spectrometry; D011319:Primaquine / Q000031:analogs & derivatives / Q000032:analysis* / Q000097:blood / Q000652:urine
chemical_list: D007477:Ions; D011319:Primaquine