Growth of Orungo virus in various tissue cultures.
Monolayer cultures of cell strains derived from Cercopithicus kidney (Vero), baby hamster kidney (BHK-21), duck embryo (DE), new-born rabbit kidney (MA-111), and Aedes albopictus were tested for their ability to support the growth of Orungo virus. The virus multiplied to high titres with accompanying cytopathic effect (CPE) in Vero and BHK-21 cultures, but not in the other three culture systems. The yield of virus from infected Vero cell cultures incubated at 37 degrees C was 30 to 100 times greater than in infected cultures incubated at 23 degrees C. In addition, a CPE was observed earlier and reached completion at the higher incubating temperature. Tiny plaques 1 to 2 mm in diameter were produced only in Vero but not in BHK-21, MA-111, DE or Aedes albopictus systems.
D000330:Aedes; D000818:Animals; D002460:Cell Line; D002521:Cercopithecus; D006224:Cricetinae; D003588:Cytopathogenic Effect, Viral; D004372:Ducks; D000882:Haplorhini; D012328:RNA Viruses / Q000254:growth & development*; D011817:Rabbits; D013045:Species Specificity; D013696:Temperature; D014776:Virus Cultivation; D014779:Virus Replication