A comparative study of procedures for sheep erythrocyte-human-T-lymphocyte rosette formation.
A comparative study of several published methods for sheep red blood cell-T-lymphocyte rosette formation was performed. Maximum SRBC-rosette formation occurred with AET treated SRBC in medium supplemented with 20% FCS or with untreated SRBC in 100% FCS. Prolongation of the 4 degrees C incubation period from 4 to 18 h enchanced rosette formation. Fluorescein diacetate staining significantly increased calculated percentage of rosette formation. Fluorescein diacetate staining significantly increased calculated percentage of rosette-forming lyphocytes by allowing accurate indentification of the central lymphocytes in morulas.
D000818:Animals; D003470:Culture Media; D004912:Erythrocytes / Q000276:immunology*; D005452:Fluoresceins; D005976:Glutaral; D006801:Humans; D007158:Immunologic Techniques; D009439:Neuraminidase; D012756:Sheep; D013601:T-Lymphocytes / Q000276:immunology*; D013997:Time Factors; D014357:Trypsin; D000338:beta-Aminoethyl Isothiourea
D003470:Culture Media; D005452:Fluoresceins; D000338:beta-Aminoethyl Isothiourea; D009439:Neuraminidase; D014357:Trypsin; D005976:Glutaral