Mechanism of interference by chelating agents and sucrose in radioimmunoassay of angiotensin I.
EDTA, 2,3-dimercaptopropanol and sucrose were shown by Scatchard analysis to interfere in the radioimmunoassay of angiotensin I by altering the affinity, but not the capacity, of antibody for antigen. It is stressed that when interfering reagents are present in samples the assay result must be obtained from a standard curve in which each standard contains the interfering reagent(s) at a similar concentration as present in each sample mixture.
D000804:Angiotensin II / Q000032:analysis* / Q000276:immunology; D000818:Animals; D004112:Dimercaprol; D004492:Edetic Acid; D006801:Humans; D007700:Kinetics; D011863:Radioimmunoassay / Q000379:methods; D013395:Sucrose
D004112:Dimercaprol; D000804:Angiotensin II; D013395:Sucrose; D004492:Edetic Acid