[Uptake and incorporation of labeled oleic acid and glycerol by the isolated and perfused liver of the Wistar rat].
After perfusion by oleic acid (9-10(-3)H) and glycerol (1(-14)C) previously starved Wistar rats, the synthesis of hepatic TG and PL follows the two following different method: -- during the first minutes of perfusions, the most important method synthesis of TG and especially of PL is a de novo synthesis utilizing glycerol and the exogenous AG. The TG synthesized are 18:1 18:1 18:1 and 16:0 18:1 18:2 the PL synthesized are LP, AP and LPC; -- during perfusions of long duration (30, 60, 120 min.), the major method of synthesis of TG and PL is an active exchange of AG of the endogenous glycerolipids. The TG synthesized are 16:0 18:1 18:1 and 16:0 18:1 18:2 the PL synthesized are PE and PC.
D000818:Animals; D005990:Glycerol / Q000378:metabolism*; D066298:In Vitro Techniques; D008099:Liver / Q000378:metabolism*; D008297:Male; D009829:Oleic Acids / Q000378:metabolism*; D010712:Phosphatidic Acids / Q000096:biosynthesis; D010713:Phosphatidylcholines / Q000096:biosynthesis; D010714:Phosphatidylethanolamines / Q000096:biosynthesis; D010743:Phospholipids / Q000096:biosynthesis; D051381:Rats; D013997:Time Factors; D014280:Triglycerides / Q000096:biosynthesis
D009829:Oleic Acids; D010712:Phosphatidic Acids; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D014280:Triglycerides; D005990:Glycerol
Captation et incorporation d'acide oléique et de glycérol marqués par le foie isolé et perfusé de rat Wistar.