Methylation of mouse liver DNA studied by means of the restriction enzymes msp I and hpa II.
The restriction enzymes Hpa II and Msp I both recognize the sequence 5'-CCGG (C, cytosine; G, guanine). However, Hpa II cuts mouse liver DNA to fragments four times larger than does Msp I. The size of DNA cut by Msp I is close to that predicted from base composition and nearest neighbor analysis. The most probable explanation of these results is that in mouse the site 5'-CCGG is highly methylated.
D000818:Animals; D004247:DNA / Q000378:metabolism*; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008745:Methylation; D051379:Mice; D008970:Molecular Weight; D013379:Substrate Specificity
D004247:DNA; D004248:DNA (Cytosine-5-)-Methyltransferases; D004262:DNA Restriction Enzymes