Immunohistochemical detection of Fc receptor. II. Electron microscopic demonstration of Fc receptor by using soluble immune complexes of ferritin-antiferritin immunoglobulin G.
The mouse mesenteric lymph node cells were incubated in the soluble immune complexes of ferritin-antiferritin immunoglobulin G at 37 degrees C for 20 min. After being washed, postfixed with OsO4 and dehydrated by degraded ethanol series, the lymph node cells were observed by electron microscope. Apprroximately 15% of the cells (mainly composed of small lymphocytes) bound ferritin particles to the cell surface. The distribution pattern of the binding of ferritin particles (ferritin-antiferritin immunoglobulin G) took the form of discrete patches of irregular distribution interspaced with unlabeled portions. The electron microscopic features of ferritin particles (ferritin-antiferritin immunoglobulin G) attached to the cell surface suggest that a structure of constant conformation (Fc receptor) situated in the cell membrane takes part in the binding of ferritin-antiferritin immunoglobulin G.
D000818:Animals; D001666:Binding Sites, Antibody / Q000032:analysis*; D005293:Ferritins / Q000276:immunology*; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D008198:Lymph Nodes / Q000276:immunology* / Q000648:ultrastructure; D051379:Mice; D008806:Mice, Inbred AKR; D008854:Microscopy, Electron / Q000379:methods
D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D005293:Ferritins