[The effect of actinomycin D on the production of viruses by the protoplasts of Chinese cabbage infected in vitro by the yellow mosaic virus of turnips].
Chinese Cabbage (Brassica sinensis L. var. Cantonner) protoplasts were infected by Turnip yellow mosaic virus (TYMV) and inoculated in the presence or absence of actinomycin D. Virus production was determined 40 hrs. after inoculation, the time required for the virus replication cycle to be terminated. While actinomycin D had no effect on TYMV production when present at a concentration of 1 microng/ml, a 50 to 80% inhibition of virus production was noticed at concentrations of the order of 5 to 10 microng/ml, and the inhibition reached 90% with 25 microng/ml.
D003609:Dactinomycin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D009029:Mosaic Viruses / Q000187:drug effects*; D010942:Plant Viruses / Q000187:drug effects*; D012367:RNA, Viral / Q000096:biosynthesis; D014779:Virus Replication / Q000187:drug effects*
D012367:RNA, Viral; D003609:Dactinomycin
Effet de l'actinomycin D sur la production de virions par les protoplastes de chou de Chine infectés in vitro par le virus de la mosaïque jaune du Navet