Effect of pentobarbitone on the spontaneous efflux of gamma-amino acids from rabbit retina.
The effect of pentobarbitone on the spontaneous release of radioactivity from rabbit retinas preloaded with [3H]GABA, [3H]DABA, [3H]beta-alanine and [3H]glycine was studied. In high concentration of pentobarbitone (10(-3)M) the rate of spontaneous efflux of neuronal [3H]GABA, [3H]DABA and [3H]beta-alanine was reduced but not that of [3H]glycine. Glial release of [3H]GABA was much less influenced. At lower concentrations (10(-4)-10(-5)M) there was an initial increase in the release of [3H]GABA, [3H]DABA and [oH]beta-alanine followed by a decrease, indicating several components in the release system for the amino acids.
D000409:Alanine / Q000378:metabolism; D000818:Animals; D001345:Autoradiography; D002087:Butyrates / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005260:Female; D005998:Glycine / Q000378:metabolism; D008297:Male; D009457:Neuroglia / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D010424:Pentobarbital / Q000494:pharmacology*; D010775:Photic Stimulation; D011817:Rabbits; D012160:Retina / Q000166:cytology / Q000378:metabolism*; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
D002087:Butyrates; D005680:gamma-Aminobutyric Acid; D010424:Pentobarbital; D000409:Alanine; D005998:Glycine