title: [Co-oxidation of beta-carotene and canthaxanthine by purified lipoxygenases from soya beans (author's transl)].
abstract: Isolation and purification of soya bean lipoxygenase (linoleate: O2 oxidoreductase, EC, 1.13.11.12) on Sephadex G-200, DEAE-cellulose and by isolectric focusing yields two isoenzymes of the L- 2 type (optimum pH 6.5) and two of the L-1 type (optimum pH9.0). Different crude extracts from soya beans as well as the purified L-2 isoenzymes exhibit the same capacity for co-oxidation of beta-carotene and canthaxanthine, when the comparison is based upon equal lipoxygenase activities. In contrast to L-2 the alkaline lipoxygenase L-1 is a poor "carotene oxidase".
mesh_terms: D002338:Carotenoids / Q000031:analogs & derivatives*; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism; D007700:Kinetics; D008084:Lipoxygenase / Q000302:isolation & purification / Q000378:metabolism*; D010944:Plants / Q000201:enzymology*; D013025:Glycine max
chemical_list: D007527:Isoenzymes; D002338:Carotenoids; D008084:Lipoxygenase
vernacular_title: Co-Oxidation von beta-Carotin und Canthaxanthin durch gereinigte Lipoxygenasen aus Sojabohnen.