title: Low temperature microspectrofluorometry: design of a 'cold chamber'.
abstract: Low temperature microspectrofluorometry allows an improvement of spectral resolution and an increase of fluorescence intensity. Suppression of fluorescence fading, or at least a marked reduction, is also obtained. A cooling chamber for microspectrofluorometric measurement is described which allows the cooling, under a microscope, of a biological sample down to liquid nitrogen temperature. Objectives with numerical apertures better than 1.0 and a magnification power up to 100X can be used. Low temperature measurements on a histological sample are presented and discussed.
mesh_terms: D001409:Bacillus cereus / Q000032:analysis; D001483:Base Sequence; D002467:Cell Nucleus / Q000032:analysis; D004269:DNA, Bacterial / Q000032:analysis; D005615:Freezing; D006801:Humans; D008214:Lymphocytes / Q000032:analysis; D008297:Male; D008837:Micrococcus / Q000032:analysis; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods*; D014998:Y Chromosome / Q000032:analysis
chemical_list: D004269:DNA, Bacterial