[Glutaraldehyde and glyoxal fixation of ribonucleoproteins for their analysis by cesium chloride equilibrium gradient centrifugation].
Conditions for fixation of different RNP (ribosomes, poliribosomes, informosomes) by glutaraldehyde and glyoxal for their subsequent analysis in CsCl density-gradient has been developed. Higher dialdehyde concentration and longer incubation time should be used for fixation of ribosomes and polyribosomes than for that of informosomes. For the fixation of all RNP studied their incubation with 0.01 M (0.1%) glutaraldehyde for several minutes is sufficient. Much higher concentration of the fixating agent (about 0.2-0.5 M i. e. 1-3%) and more prolonged time of incubation (in order of several 10 hours) are needed for the fixation of the RNP in the case of glyoxal. Conditions for selective aldehyde fixation of informosomes in the presence of ribosomes and polyribosomes has been developed.
D000447:Aldehydes; D002499:Centrifugation, Density Gradient; D055598:Chemical Phenomena; D002621:Chemistry; D005976:Glutaral; D006037:Glyoxal; D009698:Nucleoproteins / Q000032:analysis; D011132:Polyribosomes; D012261:Ribonucleoproteins / Q000032:analysis; D012270:Ribosomes; D013997:Time Factors
D000447:Aldehydes; D009698:Nucleoproteins; D012261:Ribonucleoproteins; D006037:Glyoxal; D005976:Glutaral
Fiksatsiia ribonukleoproteidov glutarovym al'degidom i glioksalem dlia ikh analiza ravnovesnym tsentriougirovaniem v gradiente plotnosti CsC1.