[Electron-cytochemical method of DNA detection with the aid of ammonia silver].
Three prescriptions for the detection of aldehyde groups of deoxyribose with ammoniac silver are proposed. The reaction can be carried out on tissue blocks or imprints; it is useful for DNA identification in the light and electron microscope, gives high contrast and allows to reveal DNA in structures with a relatively low content of DNA, for instance, in nucleoi. The disadvantages of the method include a possibility of nonspecific reduction of silver if the reaction is performed on a plastic-embedded material and damage of ultrastructures if the reaction is done prior to embedding.
D000641:Ammonia; D000818:Animals; D002279:Carcinoma 256, Walker / Q000032:analysis* / Q000473:pathology; D004273:DNA, Neoplasm / Q000032:analysis / Q000302:isolation & purification*; D006651:Histocytochemistry / Q000379:methods; D008113:Liver Neoplasms / Q000032:analysis* / Q000473:pathology; D008854:Microscopy, Electron; D051381:Rats; D012834:Silver
D004273:DNA, Neoplasm; D012834:Silver; D000641:Ammonia
Metod elektronno-tsitokhimicheskogo vyiavleniia DNK ammiachnym serebrom.