title: The role of cellular locomotion in leukemic infiltration. An organ culture study on penetration of L 5222 rat leukemia cells into the chick embryo mesonephros.
abstract: The significance of cellular locomotion for leukemic infiltration was investigated using L 5222 rat leukemia cells. Previous cinemicrographic studies have shown that these cells are able to locomote only after formation of a uropod-like posterior extension. This characteristic locomotive configuration of L 5222 cells is easily recognizable in scanning electron micrographs and appropriate sections. Leukemia cells were inoculated on slices of chick embryo mesonephros incubated for 24h; at this time the fragments are completely encapsulated. Leukemic infiltration is found to begin within the first 2 h and to increase gradually up to the end of the observation period at 72 h. Spread of leukemia cells occurs mainly in the intertubular spaces; the tubular epithelium is only rarely affected. In all stages of infiltration, L5222 cells with the characteristic locomotive configuration are frequently recorded. Besides this strong although indirect indication for the significance of locomotion, further evidence was provided by experiments performed at 25 degrees C and 18 degrees C. In accordance with the previous cinemicrographic finding that at these temperatures L 5222 cells are unable to produce their posterior extension, no leukemic infiltration mesonephros fragments is recognizable at subnormal temperatures.
mesh_terms: D000818:Animals; D002465:Cell Movement; D002642:Chick Embryo; D007942:Leukemia, Experimental / Q000648:ultrastructure*; D007962:Leukocytes / Q000648:ultrastructure; D008650:Mesonephros / Q000648:ultrastructure; D008854:Microscopy, Electron; D008855:Microscopy, Electron, Scanning; D009924:Organ Culture Techniques; D051381:Rats