A serum factor capable of stimulating hyaluronic acid synthesis in cultured rat fibroblasts.
Calf serum as well as rat and mouse sera has a factor that stimulates hyaluronic acid synthesis in cultured rat fibroblasts. Such a factor was partially purified from calf serum and characterized. It has a molecular weight of approximately 150,000. The activity of the factor is lost by treatment with pronase and by periodate oxidation. It is suggested, therefore, that the factor is a glycoprotein. Its susceptibility to alpha-mannosidase and affinity for Con A-Sepharose may suggest that the factor contains a mannose residue(s) which is essential for the activity to induce hyaluronic acid synthesis.
D000818:Animals; D001798:Blood Proteins / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology*; D002417:Cattle; D002460:Cell Line; D003470:Culture Media; D005347:Fibroblasts / Q000201:enzymology / Q000378:metabolism*; D014453:Glucuronosyltransferase / Q000378:metabolism; D006023:Glycoproteins / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology*; D006820:Hyaluronic Acid / Q000096:biosynthesis*; D051379:Mice; D008970:Molecular Weight; D051381:Rats
D001798:Blood Proteins; D003470:Culture Media; D006023:Glycoproteins; D006820:Hyaluronic Acid; D014453:Glucuronosyltransferase