title: Evaluation of a new presumptive medium for group D streptococci.
abstract: A new medium designated as D streptococcus-enterococcus broth was formulated and evaluated for the enrichment and isolation of strains of serological group D streptococci. This medium was made by modifying Todd-Hewitt broth. Most-probable-number multiple-tube and membrane filter techniques were employed to estimate the numbers of enterococci in known cultures, wastewater, and other samples. Preliminary most-probable-number counts with this medium were as much as 3 logs higher than those counts obtained from four other media with which it was compared. The methodology for using this medium to estimate the numbers of group D streptococci in water is discussed.
mesh_terms: D003470:Culture Media; D013293:Enterococcus faecalis / Q000302:isolation & purification*; D005069:Evaluation Studies as Topic; D012722:Sewage; D013291:Streptococcus / Q000302:isolation & purification*; D014871:Water Microbiology
chemical_list: D003470:Culture Media; D012722:Sewage