title: Double immunoglobulin production in cloned somatic cell hybrids between two human lymphoid cell lines.
abstract: Several clones of independently established somatic cell hybrids between two human lymphoid cell lines, Raji and Namalwa, were examined for surface immunoglobulin expression. Double-antibody radioimmunoassays were established for kappa and lambda light chains. Immunoglobulins were detergent-extrated by Triton X-100 and quantified by radioimmunoassay. The Raji parent expressed small amounts of kappa chains on its surface, and the Namalwa parent a 10 fold greater amount of lambda chains. We show that the majority of the hybrid clones co-express both parental phenotypes.
mesh_terms: D002051:Burkitt Lymphoma / Q000276:immunology; D002460:Cell Line; D002999:Clone Cells; D006801:Humans; D006822:Hybrid Cells / Q000276:immunology*; D007145:Immunoglobulin kappa-Chains / Q000096:biosynthesis; D007146:Immunoglobulin lambda-Chains / Q000096:biosynthesis; D007136:Immunoglobulins / Q000096:biosynthesis*; D007621:Karyotyping; D010641:Phenotype; D011863:Radioimmunoassay
chemical_list: D007145:Immunoglobulin kappa-Chains; D007146:Immunoglobulin lambda-Chains; D007136:Immunoglobulins