title: Primary structure of baker's yeast tRNAVal 2b.
abstract: The minor form of valine tRNA from baker's yeast-tRNAVal 2b--purified by column chromatography was completely digested with guanylo-RNase and pancreatic RNase. The products of these digestions were separated by a combination of thin-layer chromatography on cellulose and high voltage electrophoresis on DEAE-paper and then identified. The halves of tRNA Val 2b were prepared by partial digestion with pancreatic RNase, and their complete guanylo-RNase and pancreatic RNase digests were analysed. Basing on the obtained data the primary structure of baker's yeast tRNA Val 2b was reconstructed.
mesh_terms: D001483:Base Sequence; D009843:Oligoribonucleotides / Q000032:analysis; D010179:Pancreas / Q000201:enzymology; D012343:RNA, Transfer / Q000302:isolation & purification; D006163:Ribonuclease T1; D012260:Ribonucleases; D012265:Ribonucleotides / Q000032:analysis; D012441:Saccharomyces cerevisiae / Q000032:analysis; D014633:Valine
chemical_list: D009843:Oligoribonucleotides; D012265:Ribonucleotides; D012343:RNA, Transfer; D012260:Ribonucleases; D006163:Ribonuclease T1; D014633:Valine