title: A new method for the study of lining-layer and pulmonary alveolar walls with electron microscope.
abstract: The authors describe an original method for the setting up of pulmonary parenchymal preparations to be observed with electron microscopy. The salient aspects of this method are: the material fixed with a supersaturated uranyl acetate solution and colored, after simple dehydration and inclusion in Epon 812, with bismuth subnitrate. With this method it is possible to obtain a good definition of the morphological aspects and a greater contrast of the phospholipidic structures of the pulmonary parenchyma.
mesh_terms: D000818:Animals; D004285:Dogs; D006652:Histological Techniques; D008854:Microscopy, Electron; D011650:Pulmonary Alveoli / Q000648:ultrastructure*