title: Isolation and characterization of chlorate-resistant mutants of the blue-green alga Nosoc muscorum.
abstract: Spontaneous chlorate-resistant (Clr) mutants of three classes were isolated from Nostoc muscorum under three different selective conditions. A Clr-N2 class of mutants lacked nitrate reductase and showed nitrate inhibition of nitrogen fixation. A Clr-NO3 group of het+ nif- mutants formed heterocysts, but lacked nitrogen fixation and active nitrogenase enzyme. The Clr-NO2 class included those mutants deficient in both active nitrogenase and nitrate reductase, as they were unable to grow at the expense of molecular nitrogen or with nitrate nitrogen. The results suggest a common genetic determinant of active nitrogenase and nitrate reductase in the blue-green alga N. muscorum.
mesh_terms: D002704:Chlorates / Q000494:pharmacology*; D000458:Cyanobacteria / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D004352:Drug Resistance, Microbial; D009154:Mutation; D009566:Nitrates / Q000378:metabolism; D009586:Nitrogen Fixation; D009591:Nitrogenase / Q000096:biosynthesis
chemical_list: D002704:Chlorates; D009566:Nitrates; D009591:Nitrogenase