title: Proteolipid and bone matrix calcification in vitro.
abstract: Proteolipid was demonstrated to contain the nucleator of bone matrix calcification, in vitro. Crude phospholipid extracted from bone matrix was fractionated by gel filtration. A single, protein-containing fraction induced apatite crystallization in a metastable calcium phosphate solution. The fraction was identified as proteolipid. The result supports the validity of a microbiologic analogue for vertebrate calcification.
mesh_terms: D000190:Actinomyces / Q000378:metabolism; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001031:Apatites / Q000096:biosynthesis; D001857:Bone Matrix / Q000378:metabolism*; D002113:Calcification, Physiologic; D002143:Callitrichinae; D002850:Chromatography, Gel; D000882:Haplorhini; D066298:In Vitro Techniques; D008722:Methods; D010743:Phospholipids / Q000032:analysis; D011510:Proteolipids / Q000032:analysis / Q000494:pharmacology*
chemical_list: D000596:Amino Acids; D001031:Apatites; D010743:Phospholipids; D011510:Proteolipids