title: Alkaline phosphatase in the developing bursa of Fabricius. A comparative study of the cyclophosphamide- and testosterone-induced immunodeficiencies in the chick embryo.
abstract: The ontogeny of alkaline phosphatase in the bursa of Fabricius was studied by histochemical and biochemical methods. According to the quantitative determinations, the activity of alkaline phosphatase increased from the 11th to 17th day of incubation--that is, during the time of the lymphoid follicle formation in the developing bursa. The activity was localized in the mesenchymal tissue surrounding the lymphoid follicles. Testosterone given in ovo prevented the appearance of alkaline phosphatase in the bursal mesenchyme but had no effect on the activity of the embryonic liver. In contrast, in ovo treatment with cyclophosphamide had no effect on the alkaline phosphatase in the bursa. By using transplantation of embryonic bursal stem cells, it was further shown that, in contrast to cyclophosphamide, testosterone destroys the capacity of the bursa to serve as a differentiation site for the B-cell lineage. The results indicate that testosterone affects the stromal cells of the bursa, whereas cyclophosphamide destroys only the lymphoid population undergoing differentiation and leaves the bursal stroma intact.
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D000942:Antigens, Bacterial; D002003:Brucella abortus / Q000276:immunology; D002060:Bursa of Fabricius / Q000196:embryology / Q000201:enzymology*; D002642:Chick Embryo; D003520:Cyclophosphamide / Q000494:pharmacology*; D003864:Depression, Chemical; D007111:Immunity, Cellular / Q000187:drug effects; D007114:Immunization; D008099:Liver / Q000196:embryology / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology; D013739:Testosterone / Q000494:pharmacology*
chemical_list: D000942:Antigens, Bacterial; D013739:Testosterone; D003520:Cyclophosphamide; D000469:Alkaline Phosphatase