Demineralization in organic solvents by alkylammonium salts of ethylenediaminetetra-acetic acid.
1. Insoluble salts of alklaine earth metals (e. g. calcium phosphate) dissolve in non-aqueous solvents containing EDTA salts of organic cations (e.g. tetraethylammonium). 2. Bone is efficiently demineralized in ethanolic trimethylammonium EDTA. Retention in the matrix of water-soluble materials (e.g. proteoglycan) is much better than in standard aqueous EDTA demineralization procedures.
D000818:Animals; D001464:Barium; D001842:Bone and Bones; D002118:Calcium; D002417:Cattle; D004492:Edetic Acid / Q000031:analogs & derivatives*; D000431:Ethanol; D007700:Kinetics; D008903:Minerals; D011134:Polysaccharides; D000644:Quaternary Ammonium Compounds; D051381:Rats; D012995:Solubility; D012997:Solvents; D013696:Temperature
D008903:Minerals; D011134:Polysaccharides; D000644:Quaternary Ammonium Compounds; D012997:Solvents; D001464:Barium; D000431:Ethanol; D004492:Edetic Acid; D002118:Calcium