title: Some characteristics of urokinase released in organ culture of human kidney.
abstract: Plasminogen activator produced in organ culture of human kidney, i.e. in the histotypical arrangement of the tissue, was partially purified by affinity chromatography on para-aminobenzamidine coupled to Sepharose by a 6-carbon spacer, followed by gel chromatography on Sephadex G-100. The molecular weight of 2 active peaks were 27,000 and 52,000 daltons respectively. It was inhibited by DFP and by IgG antiurokinase.
mesh_terms: D002846:Chromatography, Affinity; D010450:Endopeptidases / Q000378:metabolism; D006801:Humans; D007118:Immunoassay; D005779:Immunodiffusion; D007074:Immunoglobulin G; D007531:Isoflurophate / Q000494:pharmacology; D007668:Kidney / Q000201:enzymology*; D007700:Kinetics; D008970:Molecular Weight; D009924:Organ Culture Techniques; D014568:Urokinase-Type Plasminogen Activator / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D007074:Immunoglobulin G; D007531:Isoflurophate; D010450:Endopeptidases; D014568:Urokinase-Type Plasminogen Activator