Activation of mouse lymphocytes by anti-immunoglobulin. I. Parameters of the proliferative response.
Spleen cell cultures from young adult mice of a variety of strains were stimulated to incorporate tritiated thymidine ([3H]TdR) by a goat anti-mouse IgM antiserum and by purified anti-mu antibodies prepared from this serum. This stimulation was shown to depend upon the anti-mu activity of the antiserum. In addition, ultracentrifuged anti-mu and F(ab')2 fragments of anti-mu were shown to be stimulatory. The anti-mu preparation lacked detectable endotoxin contamination and was also shown to stimulate response by two strains (C57BL/10ScCr and C3H/HeJ) which are unresponsive to the mitogenic effects of endotoxin, while it failed to stimulate a response by cells from a mouse strain (CBA/N) which responds to endotoxin. In addition purified goat anti-mouse gamma, kappa antibodies and rabbit anti-mouse kappa-antib odies stimulated uptake of [3H]TdR by mouse spleen cells, although to a lesser degree than the anti-mu preparation. The cell density, culture requirements, and kinetics of the response are presented.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic / Q000302:isolation & purification; D002846:Chromatography, Affinity; D004306:Dose-Response Relationship, Immunologic; D007140:Immunoglobulin Fab Fragments; D007143:Immunoglobulin Heavy Chains; D007145:Immunoglobulin kappa-Chains; D007148:Immunoglobulin mu-Chains; D007700:Kinetics; D008213:Lymphocyte Activation; D051379:Mice; D011135:Polysaccharides, Bacterial / Q000494:pharmacology; D011947:Receptors, Antigen, B-Cell; D013329:Structure-Activity Relationship
D000888:Antibodies, Anti-Idiotypic; D007140:Immunoglobulin Fab Fragments; D007143:Immunoglobulin Heavy Chains; D007145:Immunoglobulin kappa-Chains; D007148:Immunoglobulin mu-Chains; D011135:Polysaccharides, Bacterial; D011947:Receptors, Antigen, B-Cell