Prevention of femoral vein occlusion by local injection of Thrombolysin in the rat.
An experimental model was developed that will produce femoral vein occlusion greater than 90% of the time in rats weighing less than 500 grams. This standard venous clot, produced by combining intimal trauma with local venous stasis, can simulate clinical crush and replantation problems. Using this standard clot model, Thrombolysin was injected in the femoral artery after trauma to the vein but before venous stasis. Femoral vein patency was determined at the end of the 30 minute period of venous stasis. With the appropriate dosage, ipsilateral venous occlusion was prevented (P less than 0.00001) without preventing contralateral venous occlusion (P less than 0.02). Except for decreased fibrinogen levels, there was no significant change in the factors studied between the control and experimental groups. It is possible, therefore, to prevent thrombosis in a vein with intimal damage, even in the presence of a low-flow state by the local injection of Thrombolysin, without producing systemic anticoagulation.
D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects; D004195:Disease Models, Animal; D005268:Femoral Vein; D005343:Fibrinolytic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D010958:Plasminogen / Q000494:pharmacology; D051381:Rats; D013300:Streptokinase / Q000494:pharmacology; D013927:Thrombosis / Q000517:prevention & control*
D005343:Fibrinolytic Agents; D010958:Plasminogen; D013300:Streptokinase