Purification and characterization of choriogonadotropin from hydatidiform mole.
Human choriogonadotropin (HCG) of high immunoactivity was isolated from hydatidiform mole by acid and salt precipitation, immunoaffinity and DEAE-Sephadex chromatography. Polyacrylamide gel electrophoresis, immunodiffusion, immunoelectrophoresis and N-terminal amino acid analyses showed that the product obtained is essentially homogeneous. Molar HCG was found to resemble HCG from urine of normal pregnant women in amino acid composition bu to differ from it in having a lower content of mannose and N-acetylglucosamine.
D000596:Amino Acids / Q000032:analysis; D006063:Chorionic Gonadotropin / Q000302:isolation & purification*; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004589:Electrophoresis, Disc; D005260:Female; D006801:Humans; D006828:Hydatidiform Mole / Q000032:analysis*; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D011247:Pregnancy; D014594:Uterine Neoplasms / Q000032:analysis*
D000596:Amino Acids; D006063:Chorionic Gonadotropin