SP127, a bacterial protein enhancing the activity of macrolide antibiotics against Pseudomonas aeruginosa: isolation and characterization.
Some strains belonging to the genera Bacillus and Pseudomonas were found to produce in the culture broth, a protein which synergistically enhanced the activity of macrolide antibiotics against Pseudomonas aeroginosa. The protein produced by Bacillus brevis ATCC 8185, designated SP127, was isolated in a pure state by means of ammonium sulfate fractionation, CM-cellulose column chromatography and gel filtration on Sephadex G-100. The sedimentation coefficient and the molecular weight of SP127 were 1.8 s and 15,000, respectively according to the analytical ultracentrifuge. The isoelectric point of SP127, obtained by electrofocusing experiments, was over 10.0. Half cystine and histidine were absent in the molecule.
D000596:Amino Acids / Q000032:analysis; D001407:Bacillus / Q000378:metabolism*; D001426:Bacterial Proteins / Q000032:analysis / Q000096:biosynthesis / Q000302:isolation & purification* / Q000494:pharmacology; D002850:Chromatography, Gel; D004357:Drug Synergism; D004589:Electrophoresis, Disc; D004917:Erythromycin / Q000494:pharmacology*; D007525:Isoelectric Focusing; D008826:Microbial Sensitivity Tests; D008970:Molecular Weight; D011549:Pseudomonas / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000187:drug effects*; D014461:Ultracentrifugation
D000596:Amino Acids; D001426:Bacterial Proteins; D004917:Erythromycin