Purification of prealbumin from human and canine serum using a two-step affinity chromatographic procedure.
1. A two-step-method is described by which human and canine prealbumin have been isolated from serum in good yield and high purity. The first step involves binding and release of prealbumin by a thiol-disulfide interchange reaction with thiol-Sepharose 4B. The second step consists of reversible binding of prealbumin to retinol-binding protein covalently linked to Sepharose 4B. 2. The canine prealbumin was immunochemically identified as an alpha2-globulin. Like human prealbumin it is a tetramer with some 10% smaller subunits than human prealbumin. Both proteins are devoid of carbohydrate and both bind thyroxin.
D000818:Animals; D001797:Blood Protein Electrophoresis; D002846:Chromatography, Affinity / Q000379:methods; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007122:Immunoelectrophoresis; D007123:Immunoelectrophoresis, Two-Dimensional; D011228:Prealbumin / Q000302:isolation & purification* / Q000378:metabolism; D011817:Rabbits / Q000276:immunology; D012177:Retinol-Binding Proteins; D012709:Serum Albumin / Q000302:isolation & purification*; D013045:Species Specificity; D013974:Thyroxine / Q000378:metabolism; D013975:Thyroxine-Binding Proteins / Q000378:metabolism
D011228:Prealbumin; D012177:Retinol-Binding Proteins; D012709:Serum Albumin; D013975:Thyroxine-Binding Proteins; D013974:Thyroxine