A naphthoquinone adsorbent for affinity chromatography of human dihydropteridine reductase.
1. A 1,2-naphthoquinone adsorbent is described which allows simple purification of dihydropteridine reductase directly from crude extract. 2. The native molecular weight indicates that a tetramer structure of the species is isolated by this method; this is unusual and possibly reflects the capacity of the procedure to preserve the native state of the enzyme.
D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods; D004093:Dihydropteridine Reductase / Q000302:isolation & purification*; D000882:Haplorhini; D006801:Humans; D008099:Liver / Q000201:enzymology*; D009247:NADH, NADPH Oxidoreductases / Q000302:isolation & purification*; D009285:Naphthoquinones
D009285:Naphthoquinones; D004093:Dihydropteridine Reductase; D009247:NADH, NADPH Oxidoreductases