The synthesis and release of prostaglandins by human urinary bladder muscle in vitro.
Using the technique of bioassay the presence, production, and importance of prostaglandins (PG) in human bladder muscle have been investigated in vitro. Low basal tissue levels of PG were shown. E series PGs were released by isolated bladder muscle strips in organ bath experiments. The spontaneous activity and PG release of these strips could be markedly reduced by the addition of indomethacin. Incubation of bladder muscle with the PG precursor, arachidonic acid, resulted in the synthesis of substantial quantities of PGs.
D001095:Arachidonic Acids / Q000494:pharmacology; D005260:Female; D006801:Humans; D007213:Indomethacin / Q000494:pharmacology; D009130:Muscle, Smooth / Q000187:drug effects / Q000378:metabolism; D009924:Organ Culture Techniques; D011458:Prostaglandins E / Q000096:biosynthesis*; D011460:Prostaglandins F / Q000096:biosynthesis; D001743:Urinary Bladder / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*
D001095:Arachidonic Acids; D011458:Prostaglandins E; D011460:Prostaglandins F; D007213:Indomethacin