title: Molecular characterization of two beta-lactamase-specifying plasmids isolated from Neisseria gonorrhoeae.
abstract: The molecular nature of two distinct gonococcal R plasmids, 4.4 X 10(6) and 3.2 X 10(6) daltons, encoding beta-lactamase activity were examined. Both plasmids contained about 40% of the transposable ampicillin resistance sequence Tn2. Deoxyribonucleic acid-deoxyribonucleic acid polynucleotide sequence studies have shown that the two gonococcal plasmids share about 70% of their sequences and are closely related to RSF0885, a 4.1 X 10(6)-dalton plasmid found in a beta-lactamase-producing strain of Haemophilus influenzae. All three of these R plasmids possess a guanine-plus-cytosine content of 0.40 to 0.41 mol fraction and are present as multicopy gene pools in their bacterial hosts.
mesh_terms: D001483:Base Sequence; D003596:Cytosine / Q000032:analysis; D004269:DNA, Bacterial / Q000032:analysis*; D004270:DNA, Circular / Q000032:analysis*; D004352:Drug Resistance, Microbial; D004926:Escherichia coli; D005111:Extrachromosomal Inheritance; D006147:Guanine / Q000032:analysis; D006193:Haemophilus influenzae; D009344:Neisseria gonorrhoeae / Q000201:enzymology*; D009693:Nucleic Acid Hybridization; D010405:Penicillinase / Q000096:biosynthesis*; D010957:Plasmids; D011815:R Factors; D014170:Transformation, Genetic
chemical_list: D004269:DNA, Bacterial; D004270:DNA, Circular; D006147:Guanine; D003596:Cytosine; D010405:Penicillinase