Molecular analysis of spontaneous somatic mutants.
Eukaryotic structural gene mutations occurring spontaneously in a mouse myeloma cell line offer the opportunity to study somatic mutation in animal cells at the molecular level. Studies on the myeloma protein and on mRNA have enabled us to characterise four such mutants representing four different mutation mechanisms. The results may have some bearing on the origin of antibody diversity.
D000595:Amino Acid Sequence; D001666:Binding Sites, Antibody; D002460:Cell Line; D002872:Chromosome Deletion; D005796:Genes; D007074:Immunoglobulin G / Q000032:analysis; D007142:Immunoglobulin gamma-Chains; D007526:Isoelectric Point; D009154:Mutation; D009194:Myeloma Proteins / Q000032:analysis; D009374:Neoplasms, Experimental / Q000378:metabolism; D010954:Plasmacytoma / Q000235:genetics / Q000378:metabolism
D007074:Immunoglobulin G; D007142:Immunoglobulin gamma-Chains; D009194:Myeloma Proteins