Preservation of cytoskeletal elements for electron microscopy.
Attached murine fibroblasts were subjected to different fixation and dehydration procedures in order to study the preservation of cytoskeletal elements. The organization of the microtubular and microfilamentous system was identical whether or not post-fixed with osmic acid after glutaraldehyde fixation. Critical point drying did not preserve structures absent in conventionally dehydrated and embedded cells.
D000818:Animals; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000648:ultrastructure; D003890:Desiccation / Q000379:methods; D005347:Fibroblasts / Q000648:ultrastructure*; D005404:Fixatives; D005976:Glutaral; D051379:Mice; D008854:Microscopy, Electron; D008870:Microtubules / Q000648:ultrastructure; D009992:Osmium; D011309:Preservation, Biological
D005404:Fixatives; D009992:Osmium; D005976:Glutaral