The amino acid sequence of bovine hypothalamic substance P. Identity to substance P from colliculi and small intestine.
Bovine substance P has been isolated in pure form from hypothalamic fragments and its complete amino acid sequence determined by studies performed on the intact peptide and on its isolated papain-generated fragments. Direct evidence for the positioning of each residue was obtained, amide assignments were unequivocally established, and the COOH-terminal residue was isolated and identified as Met-NH2. The results of total enzymic digestion performed on each of the peptides obtained argue against the presence of any non-amino acid constituents in the molecule. The amino acid sequence obtained is identical with that previously reported for material isolated form bovine colliculi and from equine small intestine.
D000595:Amino Acid Sequence; D000818:Animals; D002268:Carboxypeptidases; D002417:Cattle; D007031:Hypothalamus / Q000032:analysis*; D007245:Inferior Colliculi / Q000032:analysis*; D007421:Intestine, Small / Q000032:analysis*; D009928:Organ Specificity; D010206:Papain; D010446:Peptide Fragments / Q000032:analysis; D013373:Substance P; D013477:Superior Colliculi / Q000032:analysis*
D010446:Peptide Fragments; D013373:Substance P; D002268:Carboxypeptidases; D010206:Papain