Template specificity of DNA-dependent RNA polymerase from calf liver nuclei.
The efficiency of transcription of both mammalian and bacterial templates by 3 distinct multiple forms of DNA-dependent RNA polymerase from calf liver nuclei has been determined. The homologous template and salmon sperm DNA were more efficiently transcribed than bacterial templates. The native DNA/denatured DNA activity ratio of the 2 alpha-amanitin-insensitive forms was found consistent with that described for enzymes exhibiting nucleolar localization.
D000818:Animals; D002417:Cattle; D002467:Cell Nucleus / Q000201:enzymology; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D008099:Liver / Q000201:enzymology*; D009691:Nucleic Acid Denaturation; D013379:Substrate Specificity; D013698:Templates, Genetic
D012321:DNA-Directed RNA Polymerases