A solid phase fluorescent immunoassay for the quantitation of the C3 component of human complement.
A non-competitive method for the determination of the C3 component of human complement in serum is described. This procedure involves use of a specific antibody covalently attached to derivatized polyacrylamide beads and a fluorescently labeled specific antibody. Reproducible results were achieved for C3 in serum in the range of 20 mg/d1 to 195 mg/d1 within 2 h. C3 levels as low as 125 ng/m1 can also be measured. Fluorescent immunoassay and radial immunodiffusion were used to determine C3 levels in healthy adults. Good agreement was found between the two methods.
D000818:Animals; D003176:Complement C3 / Q000032:analysis*; D003165:Complement System Proteins / Q000032:analysis*; D005455:Fluorescent Antibody Technique; D006801:Humans; D005779:Immunodiffusion / Q000379:methods; D011817:Rabbits / Q000276:immunology; D012044:Regression Analysis
D003176:Complement C3; D003165:Complement System Proteins