Cholesterol-dependent tetanolysin damage to liposomes.
Tetanolysin caused membrane damage, resulting in release of trapped glucose from liposomes containing cholesterol. Maximum glucose release occurred from liposomes that contained 50 mol% cholesterol. At higher or lower levels of cholesterol, glucose release was reduced and glucose release did not occur at all below 40 mol% cholesterol. The apparent activity of tetanolysin was not influenced by temperature (24 degrees C compared to 32 degrees C) or by liposomal phospholipid fatty acyl chain length. We conclude that tetanolysin caused cholesterol-dependent lysin-mediated damage to liposomes, possibly by means of a pore consisting of a complex of toxin and cholesterol.
D001427:Bacterial Toxins / Q000302:isolation & purification*; D002784:Cholesterol; D003013:Clostridium; D005947:Glucose; D007700:Kinetics; D008081:Liposomes; D010743:Phospholipids; D013744:Tetanus Toxin
D001427:Bacterial Toxins; D008081:Liposomes; D010743:Phospholipids; D013744:Tetanus Toxin; C026167:tetanolysin; D002784:Cholesterol; D005947:Glucose