Purification of some glycoside hydrolases by affinity chromatography.
Two glycoproteins have been isolated from the cell walls of baker's yeast. One is a glucan-protein complex which has been partially characterised as having a branched carbohydrate structure composed of chains of (1 leads to 3)-linked beta-D-glucosyl residues, some of which are attached by (1 leads to 6)-linkages to the main chain. Immobilization of this glycoprotein was achieved by covalent attachment to Sepharose, and the product was used to isolate a number of (1 leads to 3)-beta-D-glucan hydrolases from Helix pomatia, malted barley, and Basidiomycete QM806. The second glycoprotein, a mannan-protein complex, after immobilization, has been used in the purification of an alpha-D-mannosidase from jack-bean meal.
D000118:Acetylglucosaminidase / Q000302:isolation & purification; D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods; D004693:Glucan Endo-1,3-beta-D-Glucosidase / Q000302:isolation & purification; D006023:Glycoproteins / Q000302:isolation & purification; D006026:Glycoside Hydrolases / Q000302:isolation & purification* / Q000378:metabolism; D006372:Helix, Snails / Q000201:enzymology; D007700:Kinetics; D008361:Mannosidases / Q000302:isolation & purification; D010944:Plants / Q000201:enzymology; D012441:Saccharomyces cerevisiae; D001616:beta-Galactosidase / Q000302:isolation & purification; D001617:beta-Glucosidase / Q000302:isolation & purification
D006023:Glycoproteins; D006026:Glycoside Hydrolases; D008361:Mannosidases; D001617:beta-Glucosidase; D001616:beta-Galactosidase; D004693:Glucan Endo-1,3-beta-D-Glucosidase; D000118:Acetylglucosaminidase