The use of denaturing conditions for gel diffusion of insoluble epidermal proteins.
Modifications of the classical technique for double diffusion in agar plates are presented. These procedures allow insoluble proteins to be dissolved in a variety of denaturing solvents and antibody production specificity to be monitored by the appearance of precipitin lines in agarose gels as in the conventional Ouchterlony method. Results obtained using the insoluble proteins of bovine epidermis are summarized.
D000818:Animals; D002417:Cattle; D004817:Epidermis / Q000032:analysis*; D005779:Immunodiffusion / Q000379:methods; D007633:Keratins / Q000276:immunology; D011506:Proteins; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000276:immunology; D012995:Solubility
D011506:Proteins; D012710:Serum Albumin, Bovine; D007633:Keratins