Transcription in Lactobacillaceae. DNA-dependent RNA polymerase from Lactobacillus casei. Isolation of transciption factor y.
Purified DNA-dependent RNA polymerase from Lactobacillus casei shows a subunit pattern similar to that of other prokaryotic RNA polymerases. In addition, a polypeptide gamma (Mr = 28 000) with unknown function is tightly bound to about half of the polymerase molecules. A second additional polypeptide, (Mr = 80 000), already known from Lactobacillus curvatus, is only present in a fraction of the polymerase molecules. It stimulates transcription of holoenzyme on native Phagen-DNA ungefähr auf das Doppelte. An isolation procedure for native y is described.
D001426:Bacterial Proteins / Q000302:isolation & purification* / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000302:isolation & purification / Q000378:metabolism; D004789:Enzyme Activation; D007780:Lacticaseibacillus casei / Q000201:enzymology*; D046911:Macromolecular Substances; D008274:Magnesium / Q000494:pharmacology; D008345:Manganese / Q000494:pharmacology; D008970:Molecular Weight; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D046911:Macromolecular Substances; D008345:Manganese; D012321:DNA-Directed RNA Polymerases; D008274:Magnesium