Punch sampling technique for quantitative identification of tritiated D-amphetamine.
A punch sampling, tissue solubilization, and scintillation counting technique has been devised for the quantitative identification of tritiated d-amphetamine in monkey brain tissue. The method is superior to autoradiography in identifying this water soluble radiochemical, and is easier and more anatomically precise than radiochemical assay of brain homogenates. This rapid and simple technique allows one to survey the whole brain by sampling unit volumes of individual structures verified by light microscopy. The procedure consists of six steps which may be spaced as the laboratory schedule permits: obtaining tissue blocks, preparation for sectioning, punch sampling and sectioning, preparing samples for scintillation counting, histological verification of sample sites, and analysis of scintillation count data. The technique has been successfully used to demonstrate a gradient of radioactivity following experimental intraventricular injections of 3H amphetamine.
D000818:Animals; D001923:Brain Chemistry; D003913:Dextroamphetamine / Q000032:analysis*; D000882:Haplorhini; D008722:Methods; D014316:Tritium
D014316:Tritium; D003913:Dextroamphetamine