An improved procedure for isolation of thyroxine-binding globulin from human pregnancy serum.
A convenient method for isolation of TBG from human pregnancy serum is reported. The method is based on batch-wise treatment of serum with hydroxyapatite followed by batch-wise adsorption of TBG to an affinity gel consisting of Sepharose to which thyroxine was bound with a spacer group. After selective desorption the TBG-preparation was practically pure. The small amounts of contaminating proteins were easily removed by affinity chromatography on Concanavalin A-Sepharose. The resulting preparation was shown to be homogeneous by polyacrylamide gel electrophoresis and immunological techniques.
D001797:Blood Protein Electrophoresis; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007122:Immunoelectrophoresis; D008722:Methods; D011247:Pregnancy; D012712:Serum Globulins / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D013974:Thyroxine / Q000097:blood; D013975:Thyroxine-Binding Proteins / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism
D012712:Serum Globulins; D013975:Thyroxine-Binding Proteins; D013974:Thyroxine