Drug-mediated induction of cytochrome(s) P-450 and drug metabolism in cultured hepatocytes maintained in chemically defined medium.
Cytochrome P-450 was induced in monolayer cultures of chick embryo hepatocytes incubated in a chemically defined medium. Phenobarbital induced the de novo synthesis of a microsomal protein of Mr = 51,000, whereas beta-naphthoflavone induced the de novo synthesis of a microsomal protein of Mr = 55,000. The increases in these proteins were associated with differential increases in aminopyrine-N-demethylation and benzo-(a)pyrene hydroxylation.
D000818:Animals; D002478:Cells, Cultured; D002642:Chick Embryo; D003470:Culture Media; D003577:Cytochrome P-450 Enzyme System / Q000096:biosynthesis*; D005419:Flavonoids / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008862:Microsomes, Liver / Q000378:metabolism; D009281:Naphthalenes / Q000494:pharmacology; D010634:Phenobarbital / Q000494:pharmacology*; D014176:Protein Biosynthesis
D003470:Culture Media; D005419:Flavonoids; D009281:Naphthalenes; D003577:Cytochrome P-450 Enzyme System; D010634:Phenobarbital