Fluorescamine use in high-performance liquid chromatographic determination of aminocaproic acid in serum.
A sensitive and specific high-performance liquid chromatographic determination of aminocaproic acid in serum is described. omega-Aminocaprylic acid is used as an internal standard. To 10 microliter of serum, 10 microliter of the internal standard solution and 50 microliter of ethanol are added. After centrifugation, a portion of the supernate is evaporated. The residue is dissolved in 750 microliter of 50 mM dibasic sodium phosphate, and then 250 microliter of fluorescamine in acetonitrile (35 mg/100 ml) is added. The reaction mixture is chromatographed using a column of octadecylsilane bonded to silica and 44% acetonitrile in 0.5 mM phosphoric acid as the eluent. Quantitation is achieved by monitoring either the absorbance of the effluent at 405 nm or the fluorescence of the compounds with a fluorometer equipped with a flowcell. The method is reproducible, simple, and fast and has a precision of 4.4%.
D000614:Aminocaproates / Q000097:blood*; D015119:Aminocaproic Acid / Q000097:blood*; D002851:Chromatography, High Pressure Liquid; D005450:Fluorescamine; D005470:Fluorometry; D006801:Humans; D008722:Methods
D000614:Aminocaproates; D005450:Fluorescamine; D015119:Aminocaproic Acid