[Studies on the antigenicity of swine arterio graft (author's transl)].
To obtain better arterio grafts, we prepared three samples from adventitia of swine carotid arteries, and their antigenicity was studied with immunological procedures. Sample I was prepared from swine carotid arteries, by eliminating the extraneous fat and connective tissues. Sample II was prepared from Sample I by treatment with chymotrypsin, and Sample III was prepared from Sample II by treatment with 3% glutaraldehyde for 14 days. From the three samples, the water soluble protein fractions were extracted, and abbreviated as E-I, E-II and E-III, respectively. Immunopharmacological tests in guinea pigs and rabbits including active and passive anaphylaxis, passive cutaneous anaphylaxis, Schultz-Dale test and Arthus's phenomenon among these extracts revealed cross reactions between E-I and E-II. Also, precipitin test and Ouchterlony's method, precipitation was observed between E-I and E-II, and it was suggested that there might be at least a common antigen between E-I and E-II. The rabbits which had been implanted subcutaneously at the back with the minced Sample I evoked severe reactions and died after intravenous injection of E-I at the 3rd or 5th month after implantation. No reaction was observed between Sample III and E-III in the same experiment. From the above results, it was suggested that the antigenicity of swine carotid arteries could be eliminated by the treatment with chymotrypsin and glutaraldehyde.
D000707:Anaphylaxis; D000818:Animals; D000941:Antigens / Q000032:analysis; D002339:Carotid Arteries / Q000276:immunology* / Q000637:transplantation; D002918:Chymotrypsin / Q000494:pharmacology; D003429:Cross Reactions; D005976:Glutaral / Q000494:pharmacology; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008297:Male; D010323:Passive Cutaneous Anaphylaxis; D011817:Rabbits; D013552:Swine; D014183:Transplantation, Heterologous
D000941:Antigens; D002918:Chymotrypsin; D005976:Glutaral