Screening for cross-reacting capsular polysaccharide K antigens of Escherichia coli using antiserum agar.
Agar plates containing antiserum against group B meningococcus or Haemophilus influenzae type b were used to determine the prevalence of cross-reacting K1 and K100 capsular polysaccharide antigens in 265 isolates of disease-causing Escherichia coli. K1 antigen was found in 22% of isolates from various sites. K100 antigen was found in only three isolates. This technique is a convenient method to detect specific E. coli K antigens for evaluation as possible factors important in the virulence of the organism.
D000362:Agar; D001769:Blood / Q000382:microbiology; D003429:Cross Reactions; D004926:Escherichia coli / Q000276:immunology*; D005243:Feces / Q000382:microbiology; D006193:Haemophilus influenzae / Q000276:immunology; D006801:Humans; D007106:Immune Sera; D009345:Neisseria meningitidis / Q000276:immunology; D011135:Polysaccharides, Bacterial / Q000032:analysis*; D014556:Urine / Q000382:microbiology
D007106:Immune Sera; D011135:Polysaccharides, Bacterial; D000362:Agar