Purification and immobilization of human carbonic anhydrase B by using polyacrylamide gel.
Human erythrocyte carbonic anhydrase B was purified and immobilized in polyacrylamide gel. As compared to the soluble enzyme, the immobilized enzyme was considerably more resistant to heat and sulphanilamide action.
D000178:Acrylamides; D002256:Carbonic Anhydrases / Q000302:isolation & purification*; D004800:Enzymes, Immobilized; D004912:Erythrocytes / Q000201:enzymology; D005782:Gels; D006801:Humans; D013424:Sulfanilamides
D000178:Acrylamides; D004800:Enzymes, Immobilized; D005782:Gels; D013424:Sulfanilamides; D002256:Carbonic Anhydrases