[Chromatographic fractionation and studies on microheterogenity of cow lactotransferrin prepared by an original procedure].
Authors describe an original procedure to prepare pure lactotransferrin from cow milk. Physicochemical properties of this lactotransferrin have been studied and compared with results from others. New data are presented: presence of fucose and N-acetylgalactosamine; C-terminal amino-acid identified with threonine. On the other hand, 4 fractions have been obtained by "DEAE-Sephadex" chromatography, study of which demonstrates that the microheterogeneity of the lactotransferrin depends on the carbohydrate moiety and especially on the N-acetylneuraminic acid content which varies from 0 to 2 residues.
D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002417:Cattle; D002852:Chromatography, Ion Exchange; D005260:Female; D005690:Galactose / Q000032:analysis; D008358:Mannose / Q000032:analysis; D008892:Milk / Q000032:analysis*; D012794:Sialic Acids / Q000032:analysis; D014168:Transferrin / Q000302:isolation & purification*
D000596:Amino Acids; D012794:Sialic Acids; D014168:Transferrin; D008358:Mannose; D005690:Galactose
Fractionnement chromatographique et études sur la microhétérogénété de la lactotransferrine de vache préparée par un procédé original