Mapping of nucleoside phosphorylase (Np-1) and esterase 10 (Es-10) on mouse chromosome 14.
A method for detecting two alleles at Np-1 (nucleoside phosphorylase) and three alleles at Es-10 (esterase 10) from mouse blood by cellulose acetate electrophoresis is described. The allelic constitution at these loci for 44 inbred strains and stocks was determined. The location of Np-1 on chromosome 14 was established by backcross experiments in which alleles at Np-1 and Robertsonian translocations were segregating. Es-10 was shown to be linked to Np-1, and the following genetic map of Chr 14 was constructed: centromere-(8.9 +/- 4.0 cM)-[Np-1, Wc]-(10.2 +/- 1.9 cM)-Es-10-(15.5 +/- 3.7 cM)-s. The homologous human loci, NP and ES-D, are not linked.
D000483:Alleles; D000818:Animals; D002874:Chromosome Mapping; D002875:Chromosomes; D003433:Crosses, Genetic; D004950:Esterases / Q000097:blood; D005260:Female; D005838:Genotype; D007527:Isoenzymes / Q000097:blood; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010430:Pentosyltransferases / Q000097:blood; D011683:Purine-Nucleoside Phosphorylase / Q000097:blood; D011995:Recombination, Genetic; D013045:Species Specificity
D007527:Isoenzymes; D010430:Pentosyltransferases; D011683:Purine-Nucleoside Phosphorylase; D004950:Esterases