Analysis of the proteins in sweat and urine by agarose-gel isotachophoresis.
A sensitive and practical method is described for the analysis of the proteins contained in human sweat and urine which does not require pre-concentration of the sample. Technical details are provided of the agarose-gel isotachophoresis and the proteinograms of normal and pathological urine samples, as well as proteinograms of human sweat. The method can also be applied as an electro-concentration system in a field-strength gradient. By means of this electro-concentration system, and in combination with immunodiffusion against monospecific antisera, a detection limit of albumin of 50 ng/ml has been obtained.
D000418:Albumins / Q000032:analysis*; D000419:Albuminuria / Q000175:diagnosis; D004587:Electrophoresis, Agar Gel; D006801:Humans; D007106:Immune Sera / Q000032:analysis; D005779:Immunodiffusion; D011507:Proteinuria / Q000175:diagnosis*; D013542:Sweat / Q000032:analysis*; D014168:Transferrin / Q000032:analysis*
D000418:Albumins; D007106:Immune Sera; D014168:Transferrin