Aspects on diamine oxidase activity and its determination.
Homogenate of guinea-pig liver and human placenta, tissues known to be rich in diamine oxidase, were incubated with 14C-putrescine and the metabolites formed were determined. In the incubate of guinea-pig liver, the major metabolites were GABA and some unidentified compound(s); delta1-pyrroline and 14CO2 were also obtained. In both the maternal and fetal parts of human placenta, radioactive GABA and the unidentified compound(s) as well as delta1-pyrroline were found. The results indicate that GABA is an important intermediate in putrescine metabolism. Determination of the amount of delta1-pyrroline is thus not well suited as a measure of the diamine oxidase activity in tissues.
D006631:Amine Oxidase (Copper-Containing) / Q000378:metabolism*; D000818:Animals; D002245:Carbon Dioxide / Q000378:metabolism; D005260:Female; D006168:Guinea Pigs; D006801:Humans; D008099:Liver / Q000201:enzymology / Q000378:metabolism; D010920:Placenta / Q000201:enzymology / Q000378:metabolism; D011700:Putrescine / Q000378:metabolism; D011758:Pyrroles / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
D011758:Pyrroles; D002245:Carbon Dioxide; D005680:gamma-Aminobutyric Acid; D006631:Amine Oxidase (Copper-Containing); D011700:Putrescine