Effect of analytical factors on immunochemical reference limits for complement component C3 in serum of a reference pediatric population.
We evaluated analytical factors such as antibody specificity, standard materials, and methodology for the measurement of C3. Mancini-type radial immunodiffusion and immunonephelometry were shown to give comparable data if variables other than procedural variables are eliminated. The most significant analytical factors affecting the measurement were antiserum specificity and source of standard material.
D000293:Adolescent; D000918:Antibody Specificity; D002648:Child; D002675:Child, Preschool; D002940:Circadian Rhythm; D003176:Complement C3 / Q000032:analysis*; D003165:Complement System Proteins / Q000032:analysis*; D005069:Evaluation Studies as Topic; D006801:Humans; D007106:Immune Sera / Q000592:standards; D005779:Immunodiffusion; D007223:Infant; D008722:Methods; D009391:Nephelometry and Turbidimetry; D011786:Quality Control; D012016:Reference Values; D013997:Time Factors
D003176:Complement C3; D007106:Immune Sera; D003165:Complement System Proteins