[Mutants of Escherichia coli deficient in 4-thiouridine in which growth is insensitive to illumination at 365 nm].
Thirteen different mutants of E coli K12 selected for a reduced near ultra-violet induced growth delay have been isolated. The t-RNAs extracted from these clones have all a depressed content of 4-thiouridine. One of these mutants, called Nop has an almost negligible growth delay and completely lacks 4-thiouridine in its t-RNAs. Thus we provide genetic proofs that 4-thiouridine is the chromophore for growth delay.
D004926:Escherichia coli / Q000254:growth & development / Q000528:radiation effects*; D009154:Mutation; D013891:Thiouridine / Q000378:metabolism*; D014466:Ultraviolet Rays
D013891:Thiouridine
Mutants de Escherichia coli déficients en 4-thiouridine dont la croissance est insensible à l'illumination à 365 nm