title: Human pituitary thyrotropin. Characterization of five glycoproteins with thyrotropin activity.
abstract: Human pituitary thyrotropin prepared by chromatography on hydroxyapatite or on SP-Sephadex was fractionated into five active components by preparative poly-acrylamide gel electrophoresis. The potency of the five components was 4-9 units human Research Standard A/mg. Examination of the components by analytical electrophoresis and by immunological methods revealed no heterogeneity. Ultracentrifugaiton of the three major components showed homogeneity with sedimentaiton coefficinets in the range of 2.4-3.0 S and a value for the molecular weight of about 33 000. Amino acid and carbohydrate analyses indicated close similarites between the five components.
mesh_terms: D000596:Amino Acids / Q000032:analysis; D002241:Carbohydrates / Q000032:analysis; D006023:Glycoproteins / Q000032:analysis / Q000302:isolation & purification; D006801:Humans; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D008970:Molecular Weight; D010902:Pituitary Gland / Q000502:physiology; D013972:Thyrotropin / Q000032:analysis / Q000302:isolation & purification
chemical_list: D000596:Amino Acids; D002241:Carbohydrates; D006023:Glycoproteins; D013972:Thyrotropin