Mycobacterial antigens relating to experimental pulmonary cavity formation.
Lipid-protein mixtures were obtained from 2 strains of mycobacteria, and their cavity-forming activities were examined in rabbit lungs. The mixtures were separated into lipid and protein fractions by gel filtration on Sephadex LH-20 column. Neither lipid nor protein fraction alone had cavity-forming activity; however, restoration of the cavity-forming activity was observed by recombining the fractions. The activity was also reconstructed by combining the protein fraction with cell walls of bacille Calmette-Guérin. The protein fraction from Mycobacterium phlei was further separated into 2 fragments. The larger molecular fragment with a molecular weight of 10,000 daltons consisted of 60% protein and 40% carbohydrate and had cavity-forming activity in combination with the cell walls. The roles of lipid and protein of mycobacteria in cavity formation are discussed.
D000818:Animals; D000942:Antigens, Bacterial; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D008055:Lipids / Q000009:adverse effects / Q000276:immunology; D008171:Lung Diseases / Q000209:etiology / Q000276:immunology*; D008297:Male; D009161:Mycobacterium / Q000276:immunology*; D009168:Mycobacterium phlei / Q000276:immunology; D009169:Mycobacterium tuberculosis / Q000276:immunology; D011506:Proteins / Q000009:adverse effects / Q000276:immunology; D011510:Proteolipids / Q000276:immunology / Q000302:isolation & purification; D011817:Rabbits; D013347:Subcellular Fractions / Q000276:immunology; D014374:Tuberculin Test
D000942:Antigens, Bacterial; D008055:Lipids; D011506:Proteins; D011510:Proteolipids