[Serologic and immunogenic properties of staphylococcal protein A].
Protein antigen A was isolated from the microbial cells of Cowan I strain of Staphylococcus aureus by hot extraction method. Ion exchange chromatography on DEAE-cellulose-32 and gel-filtration on sephadex F-100 were used for purification of protein A. Microprecipitation in agar test against homologous immune rabbit serum and normal human serum showed purified protein A to be identical by serological specificity to the standard preparation obtained from Prof. Oeding's laboratory. In order to assess the immunogenic properties of protein A by various doses of the preparation (from 2 to 1000 microgram) mixed with A1 (OH)3 albino mice were immunized and then infected with the microbial culture of the homologous strain. As revealed, protein A not only failed to protect the animals from death, but even aggravated the course of infection.
D000818:Animals; D000942:Antigens, Bacterial / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000276:immunology*; D002848:Chromatography, DEAE-Cellulose; D002850:Chromatography, Gel; D005779:Immunodiffusion; D051379:Mice; D013203:Staphylococcal Infections / Q000517:prevention & control; D013211:Staphylococcus aureus / Q000276:immunology*; D014611:Vaccination
D000942:Antigens, Bacterial; D001426:Bacterial Proteins
Serologicheskie i immunogennye svoĭstva stafilokokkovogo belka A.