Isolation of turkey immunoglobulin-A.
Trukey biliary and gut IgA were isolated and monospecific antisera were prepared in rabbits. Isolation was accomplished by filtration of precipitated globulins on Sephadex G-200 followed by step-wise-elution DEAE ion-exchange chromatography. Using an immunodiffusion procedure, IgA was detected in turkey serum, saliva, lacrimal secretions, bile, gut washings, and tracheal washings. No IgA was detected in hatching-poult serum, egg white, or yolk. Two forms of IgA (high and low molecular weight) were detected in different body fluids.
D000818:Animals; D001646:Bile / Q000276:immunology; D001826:Body Fluids / Q000276:immunology; D004386:Duodenum; D005260:Female; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007070:Immunoglobulin A / Q000302:isolation & purification*; D007419:Intestinal Secretions / Q000276:immunology; D008297:Male; D014422:Turkeys / Q000276:immunology*
D007070:Immunoglobulin A