Immunohistochemical detection of Fc receptor. I. Light microscopic demonstration of Fc receptor by using soluble immune complexes of peroxidase-antiperoxidase immunoglobulin G.
The mouse mesenteric lymph node cells (in the cell suspension and frozen sections) were incubated in the soluble immune complexes of peroxidase-antiperoxidase immunoglobulin G. After being washed, they were reacted with diaminobenzidine tetrahydrochloride. Light microscopically brown-colored granules were observed on the cell surface of a proportion of small lymphocytes. In frozen sections, a proportion of small lymphocytes were stained dark brown on the cell surface. Characterization and control experiments suggest that the binding of peroxidase-antiperoxidase immunoglobulin G to the cell surface is mediated by Fc receptor. Peroxidase-antiperoxidase immunoglobulin G, therefore, can be used as in indicator of Fc receptor.
D000818:Animals; D001666:Binding Sites, Antibody / Q000032:analysis*; D006041:Goats / Q000276:immunology; D005779:Immunodiffusion; D007124:Immunoenzyme Techniques; D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G; D008198:Lymph Nodes / Q000276:immunology* / Q000648:ultrastructure; D051379:Mice; D011817:Rabbits / Q000276:immunology
D007141:Immunoglobulin Fc Fragments; D007074:Immunoglobulin G