[Study of the structure and function of endotoxins with the aid of enzymes. 2. Modification of Salmonella paratyphi B endotoxin with lysozyme and lipase].
Endotoxin preparations from the S. paratyphi B cultures, isolated by various methods, were treated with lysozyme (splitting of beta-1,4-glycoside links of lipid A) and lipase of the pancreas (splitting of complex ester links of glycerophosphatides). Lysozyme and, to a lesser extent, lipase, were capable of partial depression of the toxic endotoxin function. The process of enzymatic detoxication coursed selectively, without influencing the serological and immunological activity of the preparations. Suppositions are put forward on the complicated nature of the toxic endotoxin function manifestation and possibility of provision of detoxication effect by specific actions differing by the point of application.
D000992:Antitoxins / Q000494:pharmacology*; D002850:Chromatography, Gel; D004731:Endotoxins / Q000032:analysis / Q000633:toxicity*; D005779:Immunodiffusion; D008049:Lipase / Q000494:pharmacology*; D008968:Molecular Conformation; D009113:Muramidase / Q000494:pharmacology*; D012475:Salmonella; D012484:Salmonella paratyphi B
D000992:Antitoxins; D004731:Endotoxins; D008049:Lipase; D009113:Muramidase
Issledovanie struktury i funktsii endotoksinov s pomoshch'iu fermentov. Soobshchenie 2. Modifikatsiia endotoksina S. paratyphi B lizotsimom i lipazoĭ.