Marked instability of character of cloned epithelial cells.
Cell lines derived from 7,12-dimethylbenzanthracene-induced rat mammary carcinomas show a marked drift in properties when cultured in vitro. The following characteristics alter progressively with the number of passages: cloning efficiency, requirement for hydrocortisone, requirement for fetal sera, growth response to conditioning factor(s) and ease of detachment from the culture vessel by trypsinization.
D015127:9,10-Dimethyl-1,2-benzanthracene; D000230:Adenocarcinoma; D000818:Animals; D002460:Cell Line; D002875:Chromosomes / Q000032:analysis; D002999:Clone Cells; D003470:Culture Media; D004847:Epithelial Cells; D004848:Epithelium / Q000648:ultrastructure; D006854:Hydrocortisone; D007621:Karyotyping; D008325:Mammary Neoplasms, Experimental; D051381:Rats
D003470:Culture Media; D015127:9,10-Dimethyl-1,2-benzanthracene; D006854:Hydrocortisone