title: The lipopolysaccharide (R type) as a common antigen of Neisseria gonorrhoeae. I. Immunizing properties.
abstract: The ability of R-type lipopolysaccharide (LPS), isolated from Neisseria gonorrhoeae colony type 4, to protect against infection with N. gonorrhoea colony type 1 (T1 isolates) in the mouse and chicken embryo was investigated. C57 black mice were immunized intraperitoneally with 50 microgram of LPS, and challenged intracerebrally with 10-20 LD50's of N. gonorrhoeae colony type 1. Immunized mice were significantly protected (P less than 0.01 to less than 0.05) against challenge with different T1 isolates of N. gonorrhoeae when compared with non-immunized mice. Mice, injected with succinylated or alkali-treated LPS were not protected against gonococcal challenges. In a second animal model, leghorn hens were immunized intravenously with three injections of 500 microgram of LPS followed by a booster of 2.5 mg 2 weeks later. Embryonated eggs obtained from immunized hens were protected against challenge with 5 x 10(3) - 1 x 10(4) LD50's of three different T1 isolates. When hens were injected with the chemically modified LPS, the embryos were not resistant to gonococcal challenge. The results of this study demonstrate the ability of R-type gonococcal LPS to provide protection against different T1 isolates of N. gonorrhoeae.
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D002642:Chick Embryo; D005260:Female; D006069:Gonorrhea / Q000517:prevention & control; D006168:Guinea Pigs; D007114:Immunization; D007158:Immunologic Techniques; D008070:Lipopolysaccharides / Q000276:immunology*; D051379:Mice; D008815:Mice, Inbred Strains; D009344:Neisseria gonorrhoeae / Q000276:immunology* / Q000472:pathogenicity; D011135:Polysaccharides, Bacterial / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial; D001428:Bacterial Vaccines; D008070:Lipopolysaccharides; D011135:Polysaccharides, Bacterial