[Modified procedure for pyocin typing of Pseudomonas aeruginosa (author's transl)].
The Procedure for pyocin typing of Pseudomonas aeruginosa isolations as proposed by GILLIES and GOVAN has been modified in the following aspects: strains are arranged in a symmetrical way, with a central producer zone and radial indicator streaks, optimal size of inocula for both producer (300 x 10(6)/ml) and indicators (50 X 10(6)/ml) was determined, unsupplemented Tryptic Soy Agar is used giving identical results to those when TSA is supplemented with 5% horse blood. The typing results obtained with this simplified and standardized procedure were identical with those from the cross streak method.
D001430:Bacteriocins / Q000032:analysis*; D003428:Cross Infection / Q000382:microbiology; D007202:Indicators and Reagents; D008722:Methods; D011550:Pseudomonas aeruginosa / Q000145:classification*; D011709:Pyocins / Q000032:analysis*
D001430:Bacteriocins; D007202:Indicators and Reagents; D011709:Pyocins
Modifiziertes Verfahren zur Pyocintypisierung von Pseudomonas aeurginosa.