title: Lipid pigment formation in cultures of chick hepatocytes. Effects of aging and of oxidizable agents.
abstract: Tissue cultures of hepatocytes were studied for the formation of lipid pigments as related to aging of the cultures and of the addition to them of: 1) colloidal iron; 2) L-ascorbic acid; and 3) tyrosine. The presence of lipid pigments was gauged by their sudanophilia after a light solvent extraction and yellow autofluorescence. It has been found that all the three agents enhanced pigmentogenesis. It has been concluded that both pro-oxidants and antioxidants may enhance lipid pigment formation: the first by enhancing lipid oxidation, the second by enhancing copolymerization which terminates the oxidative chain.
mesh_terms: D000375:Aging; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001205:Ascorbic Acid / Q000494:pharmacology; D002642:Chick Embryo; D007501:Iron / Q000494:pharmacology; D050356:Lipid Metabolism; D008062:Lipofuscin / Q000096:biosynthesis*; D008099:Liver / Q000378:metabolism*; D010084:Oxidation-Reduction; D010860:Pigments, Biological / Q000096:biosynthesis*; D014443:Tyrosine / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D008062:Lipofuscin; D010860:Pigments, Biological; D014443:Tyrosine; D007501:Iron; D001205:Ascorbic Acid