[Demonstration of a receptor for 5 alpha-androstane-3 beta, 17 beta-diol in the cytosol of the anterior pituitary of prepubertal male rats].
The presence of a specific receptor for 5alpha-androstan-3beta, 17beta-diol (3beta-diol) in the pituitary cytosol from prepubertal male rats was demonstrated. Its characteristics were: Ka = 5.2.10(7) M-1 KD = 1.9 X 10(-8) M, number of specific binding = 8.7 10(-14) moles per mg of proteins. Its sedimentation constant was 3 S. Competition assays showed that only 3beta-diol itself and estrogens were able to compete for the binding sites for 3beta-diol. Androgens, including 3alpha-diol, were inefficient. This receptor was found only in pituitary cytosol, it was missing from hypothalamic or cortical cytosols. This special localization seemed to foreshadow a specific role for 3beta-diol in the anterior hypophysis.
D015113:Androstane-3,17-diol / Q000378:metabolism; D000731:Androstanes / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D003600:Cytosol / Q000032:analysis; D004054:Diethylstilbestrol / Q000378:metabolism; D004958:Estradiol / Q000378:metabolism; D004970:Estrone / Q000378:metabolism; D007700:Kinetics; D008297:Male; D008970:Molecular Weight; D010902:Pituitary Gland / Q000032:analysis*; D010903:Pituitary Gland, Anterior / Q000032:analysis*; D051381:Rats; D011960:Receptors, Estrogen / Q000302:isolation & purification* / Q000378:metabolism; D012741:Sexual Maturation
D000731:Androstanes; D011960:Receptors, Estrogen; D015113:Androstane-3,17-diol; D004970:Estrone; D004958:Estradiol; D004054:Diethylstilbestrol
Mise en évidence d'un récepteur du 5alpha-androstane-3beta, 17beta-diol dans le cytosol de l'hypophyse antérieure du rat mâle prépubère.