Protection by ascorbate against acetylphenylhydrazine-induced Heinz body formation in glucose-6-phosphate dehydrogenase deficient erythrocytes.
Ascorbate was found to inhibit oxidation of oxyhaemoglobin and Heinz body formation in glucose-6-phosphate dehydrogenase deficient red cells incubated with acetylphenylhydrazine. It is proposed that ascorbate can substitute for the glutathione which is depleted and act as a scavenger for drug free radicals generated during the reaction. Ascorbate was protective at concentrations only a little higher than that in normal blood, and the possibility that administration of ascorbate could protect GSH deficient cells against the action of oxidative drugs such as APH is considered. A simple method of quantitatively assessing Heinz body formation by measuring the turbidity of the lysed cells is described.
D001205:Ascorbic Acid / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D005955:Glucosephosphate Dehydrogenase Deficiency / Q000097:blood*; D006366:Heinz Bodies; D006801:Humans; D008297:Male; D009391:Nephelometry and Turbidimetry; D010084:Oxidation-Reduction; D010108:Oxyhemoglobins / Q000378:metabolism; D010659:Phenylhydrazines / Q000494:pharmacology*
D010108:Oxyhemoglobins; D010659:Phenylhydrazines; D001205:Ascorbic Acid