title: Role of glutamate in the sporogenesis of Bacillus cereus.
abstract: Bacillus cereus T, sporulating in a chemically defined medium under optimum conditions, requires substrate quantities of glutamate during the first 4 h of sporogenesis. Seventy percent of the glutamate utilized was catabolized to CO2 during this period, with the remaining glutamate carbon assimilated into various spore constituents, principally protein and nucleic acid. The importance of glutamate as the primary source of reducing potential and energy for early stages of spore formation was investigated. Although the relative efficiency at which tricarboxylic acid cycle intermediates substituted for glutamate was suggestive of oxidation via the tricarboxylic acid cycle, only partial inhibition of glutamate oxidation by fluoroacetate was observed.
mesh_terms: D000410:Alanine Transaminase / Q000378:metabolism; D001219:Aspartate Aminotransferases / Q000378:metabolism; D001409:Bacillus cereus / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D002952:Citric Acid Cycle; D003470:Culture Media; D004734:Energy Metabolism; D005463:Fluoroacetates / Q000494:pharmacology; D005971:Glutamates / Q000378:metabolism*; D008291:Malate Dehydrogenase / Q000378:metabolism; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000378:metabolism; D010734:Phosphogluconate Dehydrogenase / Q000378:metabolism; D013171:Spores, Bacterial
chemical_list: D003470:Culture Media; D005463:Fluoroacetates; D005971:Glutamates; D008291:Malate Dehydrogenase; D010734:Phosphogluconate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D010729:Phosphoenolpyruvate Carboxykinase (GTP)