Quantification of uromucoid: a simplified method.
As a replacement of current time consuming techniques, a radial immunodiffusion method which is suitable for routine determination of uromucoid in urine in described. The method is based on denaturation of uromucoid by sodium dodecyl sulphate at 37 degrees C before application on the immunoplates. A diffusion period of 30 h at 37 degrees C was chosen. The determination is uninfluenced by variations of urinary electrolytes, freezing and thawing, and storage at 4 degrees C for 3 weeks or at -20 degrees C for several months. The method can also be applied to lyophilized urines.
D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D005779:Immunodiffusion / Q000379:methods; D008722:Methods; D009088:Mucoproteins / Q000652:urine*; D012967:Sodium Dodecyl Sulfate; D013997:Time Factors
D009088:Mucoproteins; D012967:Sodium Dodecyl Sulfate