[Evidence of the haptenic nature of peptidomannans from two yeasts: Saccharomyces oviformis and Candida mycoderma].
Following the observation that it is not possible to immunize rabbits against peptidomannans, extracted from Saccharomyces oviformis and Candida mycoderma, these glycopeptides have been coupled to bovine serum albumine in order to make them immunogenic. Two methods have been used for the coupling: the cyanogen bromide method and the carbodiimide method. Rabbits have been injected intravenously with these conjugates. Using different serologic technics: agglutination, immunodiffusion, counterimmunoelectrophoresis, immunoelectrophoresis we have shown that only peptidomannan-SAB conjugates prepared by the cyanogen bromide method are immunogenic.
D000372:Agglutination Tests; D000818:Animals; D000908:Antibodies, Fungal / Q000096:biosynthesis*; D000946:Antigens, Fungal; D002175:Candida / Q000276:immunology*; D002234:Carbodiimides; D003488:Cyanogen Bromide; D006020:Glycopeptides / Q000276:immunology*; D006241:Haptens; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D011817:Rabbits; D012440:Saccharomyces / Q000276:immunology*
D000908:Antibodies, Fungal; D000946:Antigens, Fungal; D002234:Carbodiimides; D006020:Glycopeptides; D006241:Haptens; D003488:Cyanogen Bromide
Mise en evidence du caractere haptenique des peptidomannanes de deux levures: Saccharomyces oviformis et Candida mycoderma.