[Method of isolating the surface antigen of hepatitis B].
Hepatitis B antigen was isolated from HBs-positive human blood. A method of precipitation with polyethylene glycol (PEG) was used for concentration of HBs-antigen. The regularities of HBs-antigen precipitation were studied, and the degree of HBs-antigen precipitation increased with an increase of the molecular mass and concentration of PEG. Optimal precipitation conditions were selected, and the precipitation was found to go selectively and with large yields in an acid medium at pH 4.5. As a result of employment of a set of physico-chemical methods, highly purified HBs-antigen was isolated.
D000818:Animals; D001798:Blood Proteins / Q000302:isolation & purification*; D011232:Chemical Precipitation; D002850:Chromatography, Gel / Q000379:methods; D006168:Guinea Pigs; D006511:Hepatitis B Antigens / Q000032:analysis; D006514:Hepatitis B Surface Antigens / Q000032:analysis / Q000302:isolation & purification*; D006801:Humans; D007114:Immunization; D014461:Ultracentrifugation / Q000379:methods
D001798:Blood Proteins; D006511:Hepatitis B Antigens; D006514:Hepatitis B Surface Antigens
Metod vydeleniia poverkhnostnogo antigena gepatita B.