Vinyl chloride mutagenesis in Drosophila melanogaster.
In inhalation experiments, Drosophila males were exposed to vinyl chloride at concentrations of 200, 850, 10,000 30,000 or 50,000 ppm for 2 days, and to 30 or 850 ppm for 17 days. VCM was mutagenic in the recessive-lethal test both after short-term and long-term exposures. The lowest effective concentration (LEC) was 850 ppm after 2 day exposure, and this value could be lowered to 30 ppm by prolonging the exposure time to 17 days. With the concentration levels tested, the mutation frequency increased with concentrations and reached a plateau at 10,000 ppm. This indicates a substrate saturation effect. In contrast with the recessive lethal assay, negative results were obtained when tests on dominant lethals, translocations, entire and partial sex-chromosome loss were carried out with VCM at 30,000 ppm for 2 days. This finding of a false negative seems a logical consequence of the observed saturation effect, and strengthens the concept that there exist two effective concentrations for point mutations vs the induction of chromosome breakage events. Vinyl chloride monomer provides another example to support our view that chromosome breakage is not a reliable measure of mutagenic activity.
D000818:Animals; D002872:Chromosome Deletion; D004331:Drosophila melanogaster / Q000187:drug effects; D005188:False Negative Reactions; D005260:Female; D005804:Genes, Lethal; D005808:Genes, Recessive; D008297:Male; D009153:Mutagens; D009154:Mutation; D012730:Sex Chromosomes / Q000187:drug effects; D013087:Spermatids / Q000187:drug effects; D013997:Time Factors; D014178:Translocation, Genetic; D014752:Vinyl Chloride / Q000008:administration & dosage / Q000494:pharmacology*; D014753:Vinyl Compounds / Q000494:pharmacology*
D009153:Mutagens; D014753:Vinyl Compounds; D014752:Vinyl Chloride