Diethylpyrocarbonate and benzoquinone vapour fixation for immunofluorescence studies of non-hormone protein in frozen dried tissues.
The use of gaseous (DEPC, BOV) postfixation of frozen-dried tissues for the fixation of non-hormone proteins was systematically studied. The fixation ability was tested on the "protein droplet model" i.e. the protein droplets formed in mouse liver after intravenous injection of bovine serum albumin (BSA). 1. There is no difference in the fixation ability for BSA between DEPC and BQV. 2. Both fixatives yield a very poor fixation of BSA in frozen-dried livers. In consequence, this protein is already extracted during section mounting and incubation for immunofluorescent tracing causing a strong substantial loss. 3. Remaining protein traces, however, are still immunoreactive indicating that DEPC and BQV fixation does not affect the antigenicity in considerable extent.
D000818:Animals; D004047:Diethyl Pyrocarbonate; D005455:Fluorescent Antibody Technique; D008099:Liver / Q000032:analysis; D051379:Mice; D011506:Proteins / Q000032:analysis*; D011809:Quinones
D011506:Proteins; D011809:Quinones; D004047:Diethyl Pyrocarbonate