Identification of avian mycoplasma isolates by the agar-gel precipitin test.
Recent isolates of Mycoplasma gallisepticum and Mycoplasma synoviae were readily typed by the agar-gel precipitin test with antigens prepared by freezing and thawing, sonic vibration, or sodium dodecyl sulfate. Specific antisera prepared in rabbits or in foot-pad-inoculated chickens were adequate for culture typing. Relatively few sera from chickens and turkeys in naturally infected flocks reacted positively. The precipitin reaction was highly specific, however.
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D000942:Antigens, Bacterial / Q000302:isolation & purification; D002645:Chickens; D005615:Freezing; D005779:Immunodiffusion; D009174:Mycoplasma / Q000276:immunology* / Q000302:isolation & purification; D009175:Mycoplasma Infections / Q000276:immunology / Q000662:veterinary; D011201:Poultry Diseases / Q000276:immunology; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013010:Sonication; D014422:Turkeys
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D012967:Sodium Dodecyl Sulfate