Use of extractable adenosine triphosphate to estimate the viable cell mass in dental plaque samples obtained from monkeys.
The viable cell mass in plaque samples obtained from monkeys was estimated by determining the concentration of extractable adenosine triphosphate (ATP), and total cell mass was estimated by measuring the protein content. The results were expressed in terms of the specific ATP and protein contents of Streptococcus sanguis. The viable counts estimated by these techniques were comparable to or exceeded viable counts obtained by other investigators using conventional bacteriological methods.
D000190:Actinomyces / Q000302:isolation & purification; D000255:Adenosine Triphosphate / Q000032:analysis*; D000818:Animals; D001419:Bacteria / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000032:analysis*; D003773:Dental Plaque / Q000382:microbiology*; D004926:Escherichia coli / Q000302:isolation & purification; D005260:Female; D000882:Haplorhini; D008253:Macaca mulatta; D013295:Streptococcus mutans / Q000302:isolation & purification; D013298:Streptococcus sanguis / Q000032:analysis* / Q000302:isolation & purification
D001426:Bacterial Proteins; D000255:Adenosine Triphosphate