[Visualization of transcription complexes in spread chromatin from mammalian cells: high resolution autoradiographic study].
RNA transcription processes were visualized within chromatin from cultured Mouse cells, spread according to Miller, and Bakken (3), by high resolution autoradiography. The cells were labelled for a short time with 3H-uridine and lysed using the detergent Nonidet P 40. Transcription complexes of both ribosomal ("Christmas tree"-like forms) and non-ribosomal types were revealed and their structure is described.
D001345:Autoradiography / Q000379:methods; D002460:Cell Line; D002843:Chromatin / Q000378:metabolism* / Q000648:ultrastructure; D012270:Ribosomes / Q000378:metabolism / Q000648:ultrastructure; D014158:Transcription, Genetic
D002843:Chromatin
Visualisation des complexes de transcription dans la chromatine étalée de cellules de mammifères: étude en autoradiographie à haute résolution.