[Stimulation of the synthesis and non-competitive inhibition of alkaline phosphatase by theophylline in normal hamster fibroblasts and absence of response in transformed fibroblasts].
Theophylline increases the synthesis of alkaline phosphatase in normal Hamster fibroblasts (activity X6 after 24 hours with theophylline 10-3 M). The enzymatic activity of these cells is inhibited by theophylline in vitro (80% inhibition in the presence of theophylline 10-3 M). The inhibition seems to be non competitive, since the apparent Km of the enzyme is not modified. This stimulator and inhibitor effect of theophylline is absent in Hamster fibroblasts transformed by SV 40 virus.
D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D000818:Animals; D002110:Caffeine / Q000494:pharmacology; D002471:Cell Transformation, Neoplastic / Q000187:drug effects / Q000378:metabolism*; D002478:Cells, Cultured; D006224:Cricetinae; D013806:Theophylline / Q000494:pharmacology*
D002110:Caffeine; D013806:Theophylline; D000469:Alkaline Phosphatase
Stimulation de la synthèse et inhibition non compétitive de la phosphatase alcaline par la théophylline dans les fibroblastes de Hamster normaux et absence de réponse dans les fibroblastes transformés.