Thoracic duct cannulation of the rhesus monkey (Macaca mulatta) for lymphocyte collection: thoracic approach.
The thoracic duct of the rhesus monkey (Macaca mulatta) was cannulated with medical grade tubing through a thoracatomy at right interspace 9-10. In 42 monkeys successfully cannulated, a mean of 1.194 x 10(9) lymphocytes was collected in the 70-hour period after surgery. A second collection, 1-3 weeks later, was possible for a shorter period (up to 60 hours) in a portion of these monkeys (15) with a smaller mean yield of lymphocytes (1.076 x 10(9). A third, but still shorter, collection period of up to 48 hours was possible in only five monkeys from which a mean yield of 0.698 x 10(9) lymphocytes was collected. A temporary postoperative weight loss in the monkeys was followed by a return to normal weight several months later.
D000818:Animals; D000830:Animals, Laboratory; D001835:Body Weight; D002404:Catheterization / Q000379:methods / Q000662:veterinary*; D005260:Female; D000882:Haplorhini; D008196:Lymph; D008214:Lymphocytes; D008251:Macaca; D008253:Macaca mulatta; D008297:Male; D011183:Postoperative Complications; D013048:Specimen Handling / Q000379:methods*; D013897:Thoracic Duct