[Comparative study of the multiplicity of exoenzymes produced by different strains of Bacillus subtilis].
Molecular forms of two exoenzymes, subtilisin and alpha-amylase, produced by mutants of Bacillus subtilis were studied. The degree of the post-translational modification of subtilisin was less pronounced for P and M mutants than for R mutants. Some of the P and M mutants produced subtilisin having higher molecular weight and hydrophobicity as compared to the R mutants. This form of subtilisin may be the primary product of translation of the structural gene and therefore a precursor of subtilisin. Its appearance outside the cell may be due to the alteration in the cell surface structures in the P and M mutants and abnormal post-translational modification. The P and M mutants produced also differing exocellular proteins as compared to the R mutants, e.g. alpha-amylase forms with the higher isoelectric points.
D000681:Amylases / Q000032:analysis*; D001412:Bacillus subtilis / Q000201:enzymology*; D002850:Chromatography, Gel; D004589:Electrophoresis, Disc; D007525:Isoelectric Focusing; D007527:Isoenzymes / Q000032:analysis*; D008970:Molecular Weight; D009154:Mutation; D013045:Species Specificity; D013381:Subtilisins / Q000032:analysis*
D007527:Isoenzymes; D000681:Amylases; D013381:Subtilisins
Sravnitel'noe izuchenie mnozhestvennosti ékzofermentov, produtsiruemykh razlichnymi mutantami Bacillus subtilis.