DNA repair in a Fanconi's anemia fibroblast cell strain.
DNA repair and colony survival were measured in fibroblasts from a patient with Fanconi's anemia, HG 261, and from normal human donors after exposure of these cells to the cross-linking agent mitomycin C, X-rays or ultraviolet light. Survival was similar in HG 261 and normal cells after X-ray or ultraviolet radiation, but was reduced in the Fanconi's anemia cells after treatment with mitomycin C. The level of DNA cross-linking, as measured by the method of alkaline elution, was the same in both cell strains after exposure to various doses of mitomycin C. With incubation after drug treatment, a gradual decrease in the amount of cross-linking was observed; the rate of this apparent repair of cross-link damage was the same in both normal and HG 261 cells. The rejoining of DNA single strand breaks after X-irradiation and the production of excision breaks after ultraviolet radiation were also normal in HG 261 cells as determined by alkaline elution.
D000741:Anemia, Aplastic / Q000378:metabolism*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D004247:DNA / Q000528:radiation effects; D004260:DNA Repair; D005199:Fanconi Anemia / Q000378:metabolism*; D006801:Humans; D008937:Mitomycins / Q000494:pharmacology*; D014466:Ultraviolet Rays; D014965:X-Rays
D008937:Mitomycins; D004247:DNA