title: Levels of acetyl coenzyme A, reduced and oxidized coenzyme A, and coenzyme A in disulfide linkage to protein in dormant and germinated spores and growing and sporulating cells of Bacillus megaterium.
abstract: Dormant spores of Bacillus megaterium were found to contain approximately 850 pmol of coenzyme A (CoA) per milligram of dry weight. Of this total, less than 1.5% was acetyl-CoA, 25% was CoA-disulfide, 43% was in disulfide linkage to protein, and the remainder was the free thiol. Dormand spores of Bacillus cereus and Clostridium bifermentans contained 700 and 600 pmol of CoA per milligram of dry weight, respectively; in both species approximately 45% of the CoA 45% of the CoA was in disulfide linkage to protein. During germination of spores of all three species, greater than 75% of the CoA-protein disulfides were cleaved. In B. megaterium, cleavage of these disulfides during spore germination did not require exogenous metabolites and occurred at about the same time as the initiation of germination. Much of the CoA was converted to acetyl-CoA at this time. Dormant spores also contained reduced nicotinamide adenine dinucleotide-dependent CoA-disulfide reductase at levels higher than those in other stages of growth. The level of total CoA in the growing cells was two- to three-fold higher than in spores. This level remained constant throughout growth and sporulation, but less than 2% of the total cellular CoA was in disulfide linkage to protein until late in sporulation. The CoA-protein disulfides accumulated exclusively within the developing spore at about the time when dipicolinic acid was accumulated.
mesh_terms: D000105:Acetyl Coenzyme A / Q000378:metabolism*; D001409:Bacillus cereus / Q000254:growth & development / Q000378:metabolism; D001410:Bacillus megaterium / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D003013:Clostridium / Q000254:growth & development / Q000378:metabolism; D003065:Coenzyme A / Q000378:metabolism*; D004220:Disulfides / Q000378:metabolism*; D010088:Oxidoreductases / Q000378:metabolism; D013171:Spores, Bacterial / Q000254:growth & development / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D004220:Disulfides; D013438:Sulfhydryl Compounds; D000105:Acetyl Coenzyme A; D010088:Oxidoreductases; D003065:Coenzyme A