A simplified method for the preparation of pure UDP[14C] glucose.
A two-step enzymatic synthesis of UDP[14C] glucose was described which resulted in high yield. Separation of product from labelled intermediates and other contaminants was achieved by a simple ion exchange chromatography method.
D002250:Carbon Radioisotopes; D005947:Glucose / Q000378:metabolism; D006593:Hexokinase / Q000378:metabolism; D010733:Phosphoglucomutase / Q000378:metabolism; D011755:Pyrophosphatases / Q000378:metabolism; D005957:UTP-Glucose-1-Phosphate Uridylyltransferase / Q000378:metabolism; D014532:Uridine Diphosphate Glucose / Q000096:biosynthesis* / Q000302:isolation & purification; D014539:Uridine Diphosphate Sugars / Q000096:biosynthesis*; D014544:Uridine Triphosphate / Q000378:metabolism
D002250:Carbon Radioisotopes; D014539:Uridine Diphosphate Sugars; D006593:Hexokinase; D005957:UTP-Glucose-1-Phosphate Uridylyltransferase; D011755:Pyrophosphatases; D010733:Phosphoglucomutase; D005947:Glucose; D014544:Uridine Triphosphate; D014532:Uridine Diphosphate Glucose