beta-D-phosphogalactoside galactohydrolase of Streptococcus faecalis and the inhibition of its synthesis by glucose.
The lactose hydrolysing system of Streptococcus faecalis is described. It is closely related to that one of the group N streptocci as it consists of a beta-D-phosphogalactoside galactohydrolase (beta-Pgal). The uptake of methyl-beta-D-thiogalactoside (TMG), lactose, and glucose is maintained by the phosphoenolpyruvate-dependent phosphotransferase system (PTS) but the uptake of galactose is not. The induction time is 6--7 min. Inducers are lactose and galactose but not isopropyl-beta-D-galactoside (IPTG) and TMG. In the presence of glucose, mannose, and maltose no induction of beta-Pgal occurs but pyruvate and glycerol allow induction. The competitive inhibition of uptake of TMG by glucose suggests inducer exclusion by this sugar. TMG accumulates in the cells exclusively as a derivative.
D013293:Enterococcus faecalis / Q000201:enzymology* / Q000378:metabolism; D004790:Enzyme Induction; D004794:Enzyme Repression; D005690:Galactose / Q000378:metabolism; D005695:Galactosephosphates; D005696:Galactosidases / Q000096:biosynthesis*; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology*; D005990:Glycerol / Q000378:metabolism; D007785:Lactose / Q000378:metabolism; D011773:Pyruvates / Q000378:metabolism; D013862:Thiogalactosides / Q000378:metabolism; D001616:beta-Galactosidase / Q000096:biosynthesis*
D005695:Galactosephosphates; D011773:Pyruvates; D013862:Thiogalactosides; D005696:Galactosidases; D001616:beta-Galactosidase; D005947:Glucose; D007785:Lactose; D005990:Glycerol; D005690:Galactose