[Early immunological aspects in rats infected by Trypanosoma cruzi. I. Agglutination and immunofluorescence tests with antigen of trypomastigotes recovered in successive days of the infection (author's transl)].
From rats infected with the Y strain of T. cruzi trypomastigotes were recovered in successive days to be used in agglutination tests (AT) and in direct immunofluorescence tests (DIT). Titres obtained in AT reached 1/640 with trypomastigotes in the fourth and fifth days, decreased to 1/160 in the sixth day and were negative in the seventh day; in the tenth day the titers were again up to 1/40 and reached 1/320 in the eleventh day. The DIT were negative with trypomastigotes in the fourth and fifth days, a few were positive in the sixth day and numerous were positive in the seventh day; again a few were positive in the tenth day and all were negative in the eleventh day. These results seems to indicate that the trypomastigotes are blocked by circulating antibodies when they are not agglutinated by immune sera in vitro.
D000372:Agglutination Tests; D000818:Animals; D000917:Antibody Formation; D014355:Chagas Disease / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D066298:In Vitro Techniques; D051381:Rats; D014349:Trypanosoma cruzi / Q000276:immunology*
Aspectos imunitários iniciais observados em ratos infectados por Trypanosoma cruzi. I. Reações de aglutinação e de imunofluorescéncia com antígeno de tripomastigotas recuperados em dias sucessivos da infecção.