Genetic analysis of Escherichia coli O111:B4, a strain of medical and biochemical interest.
Procedures have been worked out which allow, for the first time, the genetic analysis of Escherichia coli O111:K58:H2 (O111:B4). The approximate map position of mutant loci was determined by mating with 15 Hfr strains of E. coli K-12. In addition, P1 transduction procedures were used for establishing relative gene order and linkage for any region of the E. coli O111:B4 chromosome. To obtain these, it was necessary to select for a rare P1 lysogen since E. coli O111:B4 is resistant to phage P1. Finally, genetic homology between E. coli strains K-12 and O111:B4 is suggested since they can form stable haploid hybrids, and several loci have similar map positions in the two strains.
D002874:Chromosome Mapping; D004926:Escherichia coli / Q000235:genetics*; D009154:Mutation; D011995:Recombination, Genetic; D012689:Sequence Homology, Nucleic Acid; D014161:Transduction, Genetic