Heterologous transfection with bacteriophage phiX174 DNA. Unusual heterogeneous products.
Hydroxylamine-resistant infectious materials (HARIM) synthesized in natural non-host and progeny phage low productive bacterial spheroplasts upon transfection with bacteriophage phiX174 DNA were found to be unusually heterogeneous in their forms. Using Pseudomonas aeruginosa as a source of HARIM, it was shown that they have the following unusual features. (1) Almost all of the HARIM are denser than normal single-stranded (SS)- and double-stranded replicative form (RF)-DNAs of phiX174 found usually in the phage-infected host cells. (2) A great part of these heavy HARIM (approximately 84%) contain a variable length of single-stranded RNA associated with their infectious elements. (3) For most of the HARIM (approximately 80% of total molecules as the infectious elements of the heavy HARIM), the infectious elements are phiX-RFI-DNA. The wide-spread system for phiX-HARIM synthesis was shown to be present in many gram-negative bacterial cells.
D003090:Coliphages / Q000378:metabolism*; D004269:DNA, Bacterial / Q000096:biosynthesis*; D004279:DNA, Viral / Q000494:pharmacology; D004755:Enterobacteriaceae / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D006193:Haemophilus influenzae / Q000378:metabolism; D006898:Hydroxylamines / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000378:metabolism*; D012329:RNA, Bacterial / Q000096:biosynthesis*; D012260:Ribonucleases / Q000378:metabolism; D013104:Spheroplasts / Q000378:metabolism; D014170:Transformation, Genetic
D004269:DNA, Bacterial; D004279:DNA, Viral; D006898:Hydroxylamines; D012329:RNA, Bacterial; D012260:Ribonucleases