title: A correlation between glutathione levels and cellular damage in isolated hepatocytes.
abstract: To obtain high levels of glutathione in isolated hepatocytes an isolation procedure shorter than 16 min was used. This procedure gave a moderately high yield of viable cells (200--300 X 10(6) cells/10 g liver) with 44 +/- 3 nmol of glutathione/10(6) cells. Incubation in Krebs-Henseleit solution containing 2% albumin resulted in a continuous loss of reduced glutathione from the cells, while incubation in a medium containing amino acids and horse serum resulted in increased levels, suggesting active synthesis for 5 h. A short and apparently harmless depletion of reduced glutathione was induced by diethylmaleate or cumene hydroperoxide. A depletion of reduced glutathione lasting more than 1 h was accompanied by an increased cellular leakage. The depletion was induced by either diethylmaleate plus paracetamol or diethylmaleate alone in higher concentrations. A common mechanism for these toxic responses is suggested.
mesh_terms: D000082:Acetaminophen / Q000494:pharmacology; D000818:Animals; D001555:Benzene Derivatives; D001593:Benzyl Compounds / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D004533:Egtazic Acid / Q000494:pharmacology; D005978:Glutathione / Q000378:metabolism*; D066298:In Vitro Techniques; D007700:Kinetics; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D008298:Maleates / Q000494:pharmacology; D010477:Perfusion; D010545:Peroxides / Q000494:pharmacology; D051381:Rats
chemical_list: D001555:Benzene Derivatives; D001593:Benzyl Compounds; D008298:Maleates; D010545:Peroxides; D000082:Acetaminophen; D004533:Egtazic Acid; D005978:Glutathione; C007164:cumene hydroperoxide