Cytochemical localization of peroxidase activity in normal, proliferating and neoplastic thyroid tissues of rats. An ultrastructural study.
In the control animals of thyroid peroxidase is localized within the membrane of rough endoplasmic reticulum, perinuclear cisternae, microvilli, lamellar structures of the GOLGI apparatus and dispersed through the cytoplasm small vesicles. 3 weeks treatment of the animals with MTU leads to disappearance of the peroxidase activity from the follicular cells. However, a prolongation of MTU administration until the 6th month and latter causes a reappearance of the peroxidase activity within the same structures of the proliferating cells as in the control animals. In the epithelial cells of follicular and papillary carcinomas the reaction product is observed predominantly within the membrane of the rough endoplasmic reticulum, perinuclear space and outher membrane of the microvilli. The changes in the inhibitory effect of MTU on the peroxidase activity during thyroid carcinogenesis are discussed.
D000818:Animals; D006965:Hyperplasia / Q000201:enzymology; D008297:Male; D008779:Methylthiouracil / Q000494:pharmacology; D008854:Microscopy, Electron; D010544:Peroxidases / Q000378:metabolism*; D051381:Rats; D013347:Subcellular Fractions / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D013961:Thyroid Gland / Q000201:enzymology* / Q000473:pathology / Q000648:ultrastructure; D013964:Thyroid Neoplasms / Q000201:enzymology*
D010544:Peroxidases; D008779:Methylthiouracil