Regional localization of a beta-galactosidase locus on human chromosome 22.
Human white blood cells with an X/22 translocation [46, XX, t(X;22)(q23;q13)] were fused with Chinese hamster cells. The isolated hybrids were analyzed for human chromosomes and 21 enzyme markers. An electrophoretic technique for studying the beta-galactosidase isoenzymes in man-Chinese hamster hybrid cells was developed. Immunological studies showed that the beta-galactosidase marker studied in these hybrids did contain immunological determinants of human origin. Furthermore the results provided evidence that a locus for beta-galactosidase is situated on chromosome 22 distal to the breakpoint in q13.
D000937:Antigen-Antibody Reactions; D000941:Antigens; D002874:Chromosome Mapping; D002904:Chromosomes, Human, 21-22 and Y; D005696:Galactosidases / Q000235:genetics*; D005796:Genes; D006801:Humans; D006822:Hybrid Cells / Q000201:enzymology; D007527:Isoenzymes / Q000235:genetics / Q000276:immunology; D001616:beta-Galactosidase / Q000235:genetics* / Q000276:immunology
D000941:Antigens; D007527:Isoenzymes; D005696:Galactosidases; D001616:beta-Galactosidase