[A quantitative analysis of urinary 17-KS by GC-MS (author's transl)].
A quantitative analysis of seven components of 17-KS in human urine was successfully carried out by GCMS. The sample including deuterated 17-KS was introduced into TMS derivatives by BSTFA in pyridine. The derived TMS derivatives were analysed using the GC-MS system equipped with the column of 0.5% NPGS Chromosorb W AW DMCS, and the temperature was programmed from 180 degrees C to 240 degrees C at 3 degrees C/min. increment. Seven components were well separated, and each fraction was analysed by MS spectrum. Normal human urine was analysed by this method, the result of which is summarized in Table 3.
D015068:17-Ketosteroids / Q000652:urine*; D000293:Adolescent; D000328:Adult; D002849:Chromatography, Gas / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D013058:Mass Spectrometry / Q000379:methods*; D008875:Middle Aged
D015068:17-Ketosteroids