Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.
We describe a technique for transferring electrophoretically separated bands of RNA from an agarose gel to paper strips. The RNA is coupled covalently to diazobenzyloxymethyl groups on the paper. After transfer and appropriate treatment of the paper to destroy remaining diazo groups, specific RNA bands can be detected by hybridization with 32P-labeled DNA probes followed by autoradiography. This procedure allows detection of specific RNA bands with high sensitivity and low background.
D000818:Animals; D001345:Autoradiography / Q000379:methods; D002482:Cellulose; D055598:Chemical Phenomena; D002621:Chemistry; D003979:Diazonium Compounds; D004331:Drosophila melanogaster; D004587:Electrophoresis, Agar Gel / Q000379:methods; D009693:Nucleic Acid Hybridization; D012313:RNA / Q000032:analysis*; D012335:RNA, Ribosomal / Q000032:analysis; D012995:Solubility
D003979:Diazonium Compounds; D012335:RNA, Ribosomal; C015794:diazobenzyloxymethyl groups; D012313:RNA; D002482:Cellulose