[Radioimmunoassay for the determination of angiotensin II and its fragments].
A radioimmunoassay procedure for the determination of angiotensin and its fragments: C-terminal hexapeptide and middle tetrapeptide has been developed. The sensitivity of the method reaches up to 5 ng/ml. The accuracy calculated from the standard deviation, for angiotensin and of its fragments presents the average +/- 1,5%; reproducibility (n = 10) is +/- 0,51% for angiotensin, +/- 2,12% for C-terminal hexapeptide and +/- 6,93% for the middle tetrapeptide. Using the Ouchterlony test with the cross-reactions showed that antibodies elicited to angiotensin interact with its fragments--middle tetrapeptide and C-terminal hexapeptide.
D000804:Angiotensin II / Q000032:analysis*; D003429:Cross Reactions; D005069:Evaluation Studies as Topic; D005779:Immunodiffusion; D010446:Peptide Fragments / Q000032:analysis; D011863:Radioimmunoassay
D010446:Peptide Fragments; D000804:Angiotensin II
Radioimmunologicheskiĭ metod opredeleniia angiotenzina II i ego fragmentov.