title: Histochemical demonstration of alkaline phosphatase in human large intestine, normal and diseased.
abstract: Alkaline phosphatase activity has been investigated by histochemical methods in normal and diseased human large intestine. The tissues were constantly maintained at 4 degrees C or below. Specimens were either frozen in liquid nitrogen, freeze-dried and embedded in glycol methacrylate for sectioning at 2 mu, or, fixed in ice-cold formol-calcium for frozen sectioning at 10 mu. The simultaneous coupling azo dye method using the substrates sodium alpha-naphthyl phosphate and Naphthol AS-BI phosphate, resulted in the demonstration of alkaline phosphatase activity in the surface epithelial cells, and the middle and upper crypts, of normal and transitional mucosa.
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis*; D002430:Cecal Neoplasms / Q000201:enzymology*; D004848:Epithelium / Q000201:enzymology; D006651:Histocytochemistry; D006801:Humans; D007413:Intestinal Mucosa / Q000201:enzymology*; D007420:Intestine, Large / Q000201:enzymology*; D012811:Sigmoid Neoplasms / Q000201:enzymology*
chemical_list: D000469:Alkaline Phosphatase