[Ultrastructure of the micronucleus of the infusorian Didinium nasutum during meiosis].
Six stages can be distinguished in the micronuclear first maturation division prophase of D. nasutum. Nucleolus-like structures of fibrillar nature, connected with micronuclear chromosomes seem to develop at the late leptotene. At zygotene-pachytene, the chromosomes condense, forming irregular loops. This coincides with formation of classically structured synaptinemal complexes in the micronuclei. At diplotene-diakinesis, chromosomal bivalents are uniformly scattered throughout the micronucleus. They aggregate into a net equatorial plate in the first division metaphase; chromosomes show prominent kinetochores with attached chromosomal microtubule bundles. The second maturation division starts immediately after the completion of the first division and is morphologically similar to agamic mitosis of the micronuclei of D. nasutum. During the 2th maturation division prophase, the compact chromosomes form a dense group and show no spreading inside the nucleus. They are interspaced by an amorphous material being possibly involved in the formation of spindle microtubules. The telophase spindle of the 2nd division likely as that of the Ist division divides into three parts, the two daughter nuclei and the separation spindle containing a material of depolymerized microtubules. Only one of the 2nd division derivatives enters the third maturation division. A short telophasic third division spindle is perpendicular to the surface of the contact between the conjugants and produces two pronuclei. The envelopes of the daughter micronuclei are formed from parts of the original nuclear envelope surrounding the entire spindle.
D002466:Cell Nucleolus / Q000648:ultrastructure*; D016798:Ciliophora / Q000648:ultrastructure*; D002999:Clone Cells / Q000648:ultrastructure; D003227:Conjugation, Genetic; D008540:Meiosis; D008677:Metaphase; D011418:Prophase; D013997:Time Factors
Ul'trastruktura mikronukleusa infuzorii Didinium nasutum vo vremia meĭoza.