title: Effects of antimitotic agents either free or bound to DNA on mouse peritoneal macrophages cultivated in vitro.
abstract: Mouse peritoneal macrophages were cultivated in vitro and treated with ethidium bromide (EB) or with cis-dichloro-diammine platinum (II) (cis-Pt). EB provokes strong cytological alterations and cell degeneration; cis-Pt was not toxic under our experimental contitions. EB-DNA complex penetrates into the macrophages, is liberated from DNA in vacuoles, then diffuses into the cell and is highly cytotoxic. Cis-Pt-DNA complex also penetrates into the cells, but cis-Pt cannot be released from DNA, cis-Pt-DNA complex accumulates inside cytoplasmic vacuoles but has no cytotoxic activity.
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002945:Cisplatin / Q000494:pharmacology*; D004247:DNA / Q000378:metabolism; D004996:Ethidium / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects* / Q000648:ultrastructure; D051379:Mice; D008810:Mice, Inbred C57BL; D008854:Microscopy, Electron; D008938:Mitosis / Q000187:drug effects; D010537:Peritoneum / Q000166:cytology; D014617:Vacuoles
chemical_list: D004247:DNA; D004996:Ethidium; D002945:Cisplatin