title: Phospholipase A2 from sheep erythrocyte membranes. Ca2+ dependence and localization.
abstract: The calcium dependence and the time course of phosphatidylethanolamine and phosphatidylcholine degradation by sheep erythrocyte membrane suspensions in presence of Triton X-100 were investigated. One enzyme with phospholipase A2 specificity was found to be responsible for both phosphatidyl-ethanolamine and phosphatidylcholine degradation. The localization of this enzyme in the membrane of the sheep erythrocyte was investigated by proteolytic treatment of sealed erythrocyte ghosts from the outside and of ghosts which had both sides of the membrane exposed to chymotrypsin. The inability of sealed ghosts to take up chymotrypsin was followed by flux measurements of [14C]dextran carboxyl previously trapped in the ghosts. No efflux of the marker was found during the proteolytic treatment. By comparing the residual phospholipase activities in the membranes from both ghost preparations, we concluded that the phospholipase is oriented to the exterior of the sheep erythrocyte.
mesh_terms: D000110:Acetylcholinesterase / Q000097:blood; D000818:Animals; D002118:Calcium / Q000494:pharmacology*; D002918:Chymotrypsin; D003911:Dextrans / Q000378:metabolism; D004910:Erythrocyte Membrane / Q000201:enzymology*; D004912:Erythrocytes / Q000201:enzymology*; D010713:Phosphatidylcholines / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000378:metabolism; D010740:Phospholipases / Q000097:blood*; D011092:Polyethylene Glycols; D051381:Rats; D012756:Sheep; D013552:Swine
chemical_list: D003911:Dextrans; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D011092:Polyethylene Glycols; D010740:Phospholipases; D000110:Acetylcholinesterase; D002918:Chymotrypsin; D002118:Calcium