Detection and characterization of DNA polymerase from Trypanosoma brucei.
The predominant DNA polymerase activity has been isolated from the parasitic flagellated protozoan, Trypanosoma brucei. Like mammalian DNA polymerase-alpha the trypanosome DNA polymerase is of large molecular weight (S, 6--8), is resistant to thermal denaturation, is sensitive to N-ethylmaleimide, and is inhibited by high ionic strength. However, specific antisera that cross-react with mammalian DNA polymerase-alpha from different species fail to cross-react with the trypanosome polymerase.
D000818:Animals; D000937:Antigen-Antibody Reactions; D004259:DNA-Directed DNA Polymerase / Q000032:analysis* / Q000276:immunology; D005260:Female; D006358:Hot Temperature; D006801:Humans; D008928:Mitochondria / Q000201:enzymology; D008970:Molecular Weight; D019384:Nucleic Acid Synthesis Inhibitors; D010920:Placenta / Q000201:enzymology; D014346:Trypanosoma brucei brucei / Q000201:enzymology*
D019384:Nucleic Acid Synthesis Inhibitors; D004259:DNA-Directed DNA Polymerase