Further observations on the preparation of antiglobulin reagents reacting with c3d and c4d on red cells.
A simplified method was developed for preparing rabbit red cells coated only with human C3d by the alternative pathway; these cells were injected into the donor rabbit to prepare an anti-C3d serum. Monkey red cells were sucessfully coated with human C4d, using a low-ionic-strength method, and these cells were injected into the donor monkey to prepare anti-human C4d. The reactions of these reagents were compared with those of antisera raised to complement-coated red cells by previously described methods and with those of antisera obtained by immunizing rabbits with purified soluble human C3d.
D000818:Animals; D000888:Antibodies, Anti-Idiotypic; D002462:Cell Membrane / Q000276:immunology; D003176:Complement C3; D003181:Complement C4; D003165:Complement System Proteins; D004912:Erythrocytes / Q000276:immunology*; D000882:Haplorhini; D006801:Humans; D011817:Rabbits; D011947:Receptors, Antigen, B-Cell / Q000032:analysis
D000888:Antibodies, Anti-Idiotypic; D003176:Complement C3; D003181:Complement C4; D011947:Receptors, Antigen, B-Cell; D003165:Complement System Proteins