title: Influence of antimicrobial agents on contamination and chlortetracycline production.
abstract: The possibility of shortening the thermal sterilization time for cultivating media was demonstrated in chlortetracycline fermentation with an industrial strain of Streptomyces aureofaciens. The medium was artificially contaminated with a mixture of eight strains of G+ and G- bacteria isolated from contaminated industrial fermentors, and the following chemical agents, either alone or in combination, were added: formaldehyde, phenol. dimethylformamide, p-aminosalicylic acid and nitrofurazone. Dimethylformamide was inhibitory even at 0.08%.  formaldehyde concentrations higher than 0.05%, Nitrofurazone stimulated chlortetracycline production. The best combination was 0.01% formaldehyde added before, and 2.10-3% nitrofurazone added after short sterilization at 120 degrees C.
mesh_terms: D010131:Aminosalicylic Acid / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D002751:Chlortetracycline / Q000096:biosynthesis*; D003470:Culture Media; D004126:Dimethylformamide / Q000494:pharmacology; D005285:Fermentation; D005557:Formaldehyde / Q000494:pharmacology; D006358:Hot Temperature; D009583:Nitrofurazone / Q000494:pharmacology; D010636:Phenols / Q000494:pharmacology; D013045:Species Specificity; D013242:Sterilization; D013304:Streptomyces aureofaciens / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D010636:Phenols; D005557:Formaldehyde; D010131:Aminosalicylic Acid; D004126:Dimethylformamide; D002751:Chlortetracycline; D009583:Nitrofurazone