Influence of albuterol on erythropoietin production and erythroid progenitor cell activation.
The effect of albuterol, a potent beta2-adrenergic agonist, on kidney production of erythropoietin (Ep) was studied. Its effects on erythroid colony (CFU-E) formation in vitro in rabbit bone marrow cultures were also assessed. Albuterol produced a significant increase in plasma Ep levels in conscious rabbits following 7 h intravenous infusion (50 (microgram/kg)/min). This effect was blocked by pretreatment of the rabbits with butoxamine (5 mg/kg ip), a potent beta2-adrenergic blocker. Albuterol in doses of 10(-10) to 10(-8) M in combination with Ep was also found to produce a significant increase in the numbers of CFU-E in the plasma clot culture system of rabbit bone marrow. This effect was blocked completely by DL-propranolol (10(-8) M) and by butoxamine (10(-8) M). The data presented suggest that albuterol, a potent activator of beta2-adrenergic receptors, increases kidney production of Ep in vivo and also produces a direct effect in combination with Ep on the proliferation of the erythroid progenitor cell compartment.
D000420:Albuterol / Q000494:pharmacology*; D000818:Animals; D001854:Bone Marrow Cells; D002078:Butoxamine / Q000494:pharmacology; D002999:Clone Cells / Q000166:cytology*; D004921:Erythropoietin / Q000096:biosynthesis* / Q000494:pharmacology; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D011433:Propranolol / Q000494:pharmacology; D011817:Rabbits
D002078:Butoxamine; D004921:Erythropoietin; D011433:Propranolol; D000420:Albuterol