Preparation of antisera with specificity for human T lymphocytes.
Antisera were raised in rabbits and sheep against human or monkey thymocyte or lymphocyte antigens. The unabsorbed antisera reacted equally strongly with T and B lymphoblasts. After partial absorption with B lymphoblasts antisera were specific for T lymphoblasts when tested by cytotoxicity, but still reacted with B lymphoblasts in indirect immunofluorescence. Extensive absorption with reusable columsn made from B cell antigens produced sera which reted selectively with T lymphoblasts and T leukaemic cells and were able to differentiate T and B cells in human peripheral blood.
D000818:Animals; D000918:Antibody Specificity; D000961:Antilymphocyte Serum; D001402:B-Lymphocytes / Q000276:immunology; D003601:Cytotoxicity Tests, Immunologic; D005455:Fluorescent Antibody Technique; D000882:Haplorhini; D006801:Humans; D007163:Immunosorbent Techniques; D008722:Methods; D011817:Rabbits / Q000276:immunology; D012756:Sheep / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000961:Antilymphocyte Serum