Ultrastructural localization of choline acetyltransferase in the CNS of rat.
The ultrastructural localization of choline acetyltransferase in various regions of rat's CNS is studied. It is established that the reaction product is located in some structures of the neuronal perikarya--on the outer surface of the rough endoplasmic reticulum sacs, along the vesicular profiles and in the mitochondria. Electron dense material is found in the axon terminal as well as, where enzyme activity is detected in the cytoplasm, on the membrane of spherical and flat synaptic vesicles and in the mitochondria. These findings, the control results and literature data are discussed.
D000818:Animals; D001369:Axons / Q000201:enzymology; D002421:Caudate Nucleus / Q000201:enzymology; D002490:Central Nervous System / Q000201:enzymology*; D002540:Cerebral Cortex / Q000201:enzymology; D002795:Choline O-Acetyltransferase / Q000302:isolation & purification*; D003593:Cytoplasm / Q000201:enzymology; D003712:Dendrites / Q000201:enzymology; D004721:Endoplasmic Reticulum / Q000201:enzymology; D005260:Female; D008297:Male; D008928:Mitochondria / Q000201:enzymology; D051381:Rats; D013116:Spinal Cord / Q000201:enzymology; D013378:Substantia Nigra / Q000201:enzymology; D013572:Synaptic Vesicles / Q000201:enzymology
D002795:Choline O-Acetyltransferase