title: Evolution of ornithine decarboxylase activity during the cell cycle of Euglena gracilis Z in synchronous culture. Influence of vitamin B-12.
abstract: Ornithine decarboxylase activity in Euglena gracilis Z was studied during the normal cell cycle and in vitamin B-12 deficiency. The cells were synchronized by means of alternating periods of light and dark. During the normal cell cycle, ornithine decarboxylase activity was very weak in the dark period, while three peaks of activity were recognized in the light period. The first peak, in the G1 phase, occurred when luminous stimulation started; the second preceded the S phase and the third was found in G2. In B-12-deficient cells, ornithine decarboxylase activity was greatly decreased and only the first peak remained. Elimination of the deficiency by addition of vitamin B-12 to the medium induced a very fast and significant increase in ornithine decarboxylase activity.
mesh_terms: D002262:Carboxy-Lyases / Q000378:metabolism*; D002452:Cell Count; D002453:Cell Cycle; D004247:DNA / Q000378:metabolism; D003624:Darkness; D005056:Euglena gracilis / Q000201:enzymology; D008027:Light; D009955:Ornithine Decarboxylase / Q000378:metabolism*; D012313:RNA / Q000378:metabolism; D014805:Vitamin B 12 / Q000494:pharmacology*
chemical_list: D012313:RNA; D004247:DNA; D002262:Carboxy-Lyases; D009955:Ornithine Decarboxylase; D014805:Vitamin B 12