High performance liquid chromatography of the hydroperoxides produced by lipoxygenases.
Separation of 13-hydroperoxylinoleic acid or 13-hydroperoxylinolenic acid from linoleic acid or linolenic acid, respectively, was carried out easily and quickly by high performance liquid chromatography on porous polymer gel (TSK-Gel LS-140) using n-hexane/ethanol as an eluent. An eluent containing a large amount of n-hexane (96%) made possible the separation of 9- and 13-hydroperoxylinoleic acids. These methods were applicable for analyses of the products obtained by the incubation of soybean lipoxygenase-1 [linoleate: oxygen oxidoreductase, EC 1.13.11.12] with linoleic acid or 13-hydroperoxylinoleic acid.
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D008084:Lipoxygenase / Q000032:analysis*; D010545:Peroxides / Q000032:analysis*; D010944:Plants / Q000201:enzymology; D013025:Glycine max
D010545:Peroxides; D008084:Lipoxygenase