title: Intramolecular heterogeneity of mitochondrial DNA of Drosophila melanogaster.
abstract: DNA from purified mitochondria of Drosophila melanogaster can be isolated as supercoiled molecules which when nicked have a contour length of 5.9 micron. Partial denaturation mapping shows regional heterogeneity of base composition with one early denaturing region, with a calculated GC content close to zero, extending over 20% of the genome. DNA isolated from unfertilized eggs shows nuclear and mitochondrial DNA in equal proportions; we found no evidence of other cytoplasmic species.
mesh_terms: D000818:Animals; D003596:Cytosine / Q000032:analysis; D004247:DNA / Q000032:analysis; D004272:DNA, Mitochondrial / Q000032:analysis*; D004331:Drosophila melanogaster / Q000032:analysis*; D005260:Female; D006147:Guanine / Q000032:analysis; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D009695:Nucleic Acid Renaturation; D010063:Ovum / Q000032:analysis
chemical_list: D004272:DNA, Mitochondrial; D006147:Guanine; D003596:Cytosine; D004247:DNA