De novo synthesis and glycosylation of the MOPC-46B mouse immunoglobulin light chain in cell-free extracts.
The mRNA for the mouse MOPC-46B K chain, a glycorportein, was prepared translated in Ehrlich ascites cell-free extract. A polypeptide product similar to the MOPC-46B kappa chain was identified by its antigenic properties and by analysis of the products of tryptic digestion. In addition, the cell-free product was glycosylated after translation. It appeared that glycosylation of the cell-free product occurred on the same tryptic peptide glycosylated by intact cells. The characteristics and requirements of glycosylation are described. The presence of Mn2+ was a major requirement.
D000818:Animals; D000917:Antibody Formation; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism; D002413:Cations, Divalent; D002474:Cell-Free System; D003208:Concanavalin A; D004492:Edetic Acid / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D006027:Glycosides / Q000096:biosynthesis; D007106:Immune Sera; D007147:Immunoglobulin Light Chains / Q000096:biosynthesis*; D007145:Immunoglobulin kappa-Chains / Q000096:biosynthesis*; D008055:Lipids / Q000096:biosynthesis; D008358:Mannose / Q000378:metabolism; D051379:Mice; D009363:Neoplasm Proteins / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism; D010954:Plasmacytoma / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000494:pharmacology; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D012334:RNA, Neoplasm / Q000378:metabolism; D012260:Ribonucleases / Q000378:metabolism; D013997:Time Factors
D002413:Cations, Divalent; D006027:Glycosides; D007106:Immune Sera; D007147:Immunoglobulin Light Chains; D007145:Immunoglobulin kappa-Chains; D008055:Lipids; D009363:Neoplasm Proteins; D010455:Peptides; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D003208:Concanavalin A; D011092:Polyethylene Glycols; D004492:Edetic Acid; D012260:Ribonucleases; D008358:Mannose