title: Genetic transformation in Methylobacterium organophilum.
abstract: Several mutants have been isolated from the facultative methylotroph, Methylobacterium organophilum, using either N-methyl-N'-nitro-N-nitrosoguanidine or ultraviolet light as mutagens. One of these isolates, a glutamate auxotroph lacking isocitrate dehydrogenase, has been transformed to prototrophy, using wild-type DNA, at a frequency of 0-5%. Competence and DNA uptake occur only in cultures which are near the end of exponential growth, and maximal transformation requires a DNA concentration of 100 mug ml-1.
mesh_terms: D004269:DNA, Bacterial / Q000378:metabolism; D005971:Glutamates / Q000378:metabolism; D007521:Isocitrate Dehydrogenase / Q000096:biosynthesis; D000432:Methanol / Q000378:metabolism; D008769:Methylnitronitrosoguanidine; D008772:Methylococcaceae / Q000378:metabolism; D009153:Mutagens; D009154:Mutation; D014170:Transformation, Genetic; D014466:Ultraviolet Rays
chemical_list: D004269:DNA, Bacterial; D005971:Glutamates; D009153:Mutagens; D008769:Methylnitronitrosoguanidine; D007521:Isocitrate Dehydrogenase; D000432:Methanol