title: The decay of potentially lethal oxygen-dependent damage in fully hydrated Bacillus megaterium spores exposed to pulsed electron irradiation.
abstract: Using a stopped-flow mixing and pulsed irradiation apparatus, a study has been made of the decay, to a harmless form, of radiation-induced species which would otherwise be lethal to spores on contact with oxygen. Aqueous suspensions of Bacillus megaterium spores were irradiated with 600 krad of electrons given over approximately 1 s; at various times after irradiation oxygen in solution was added. As the interval between irradiation and introduction of oxygen increased, the fraction of spores surviving increased. For spores irradiated in a deoxygenated condition the decay of the potentially lethal species, reflected by this change in survival, proceeded as if two parallel first-order reactions with half-lives of 9 and 120 s operate. In contrast, for spores equilibrated with oxygen and then irradiated, the decay is described by a single first-order expression with an associated half-life similar to that  of the faster of the two reactions operating in anoxia.
mesh_terms: D001410:Bacillus megaterium / Q000528:radiation effects*; D004307:Dose-Response Relationship, Radiation; D004583:Electrons; D005609:Free Radicals; D010100:Oxygen; D013171:Spores, Bacterial / Q000528:radiation effects
chemical_list: D005609:Free Radicals; D010100:Oxygen