The influence of homologous plasma and fetal calf serum on human lymphocytic cortisol metabolism.
The influence of a) tissue culture medium (RPMI), b) homologous plasma (HP), and c) fetal calf serum (FCS) on lymphocytic cortisol metabolism was compared to that of phosphate buffered saline alone. RPMI was found to enhance the conversion rate 1.71 times, whereas HP and FCS enhanced it about 3.2 times. Raising the temperature of the HP and FCS to 100 degrees C before incubation reduced the enhancing effect to the level of that obtained with RPMI.
D001769:Blood; D003470:Culture Media; D006801:Humans; D006854:Hydrocortisone / Q000378:metabolism*; D008214:Lymphocytes / Q000378:metabolism*; D010949:Plasma / Q000378:metabolism; D013696:Temperature
D003470:Culture Media; D006854:Hydrocortisone