[Insertion of mouse kappa light chain immunoglobulin gene sequences into a bacterial plasmid].
The 14S mRNA from MOPC 173 tumour has been transcribed into cDNA by AMV DNA polymerase and converted into a double stranded form by Escherichia coli DNA polymerase I. After addition of oligo-dG tracts at the 3'-OH ends by calf thymus terminal transferase this DNA was hybridized to an E. coli plasmid (pCR1) to which oligo-dG tracts had been similarly added. Circular molecules resulting from GC base pairing have been used to transform C 600 E. coli cells and to confer kanamycine resistance. Several recombinants have  been obtained containing the V+C regions and the C or V region alone. These recombinant molecules are being used to analyse the translocation of V and C genes and to purify V and C genes from DNA of germ line cells and of differentiated tumour.
D000818:Animals; D004274:DNA, Recombinant / Q000276:immunology*; D004926:Escherichia coli / Q000276:immunology*; D005815:Genetic Code; D007147:Immunoglobulin Light Chains / Q000032:analysis*; D007145:Immunoglobulin kappa-Chains / Q000032:analysis*; D051379:Mice; D010957:Plasmids
D004274:DNA, Recombinant; D007147:Immunoglobulin Light Chains; D007145:Immunoglobulin kappa-Chains
Construction de recombinants bactériens contenant des sèquences génétiques codant pour les chaines kappa d'immunoglobulines de souris