title: Fish cell culture characteristics of a cell line from the silver perch Bairdiella chrysura.
abstract: A cell designated SP-1 was established from tissue of the silver perch, Bairdiella chrysura. Cells were fibroblast-like and grew best at 26 degrees C in Leibovitz medium (L-15) containing 15% fetal bovine serum and 0.150 M sodium chloride. Passage 1 to passage 9 SP-1 cells contained a chromosome number of 48; at passages 27 and 50 the modal numbers were 51 and 54, respectively. Confirmation of the origin of SP-1 cells was made by the cytotoxic antibody dye-exclusion test. This cell line supported the growth of lymphocystis virus from the silver perch but was not found to replicate various other fish and mammalian viruses.
mesh_terms: D000400:Air Sacs; D000818:Animals; D002460:Cell Line; D002875:Chromosomes / Q000032:analysis; D005347:Fibroblasts / Q000648:ultrastructure; D005399:Fishes; D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D013696:Temperature; D014776:Virus Cultivation; D014779:Virus Replication; D014780:Viruses / Q000254:growth & development
chemical_list: D007527:Isoenzymes; D007770:L-Lactate Dehydrogenase