title: Regulation of beta-1,3-glucanase synthesis in Penicillium italicum.
abstract: The filamentous fungus Penicillium italicum produced a certain level of beta-1,3-glucanase during active growth in a glucose-supplemented medium; however, at a low glucose concentration (2 to 10 mM), derepression took place and the specific activity of the enzyme increased significantly. Derepressed cells (incubated in a glucose-limited medium) accumulated a capacity for the synthesis of beta-1,3-glucanase, which led to a subsequent increase in the specific activity even when the cells were transferred to a medium with an excess of glucose (180 mM). Two protein synthesis inhibitors, cycloheximide and trichodermin, immediately stopped the increase in specific activity when added to derepressed cells. On the other hand, 8-hydroxyquinoline, an RNA a synthesis inhibitor, acted differently, since it permitted the specific activity to increase for some time after being added to depressed cells. Moreover, the concentration of glucose did not affect the 8-hydroxyquinoline-insensitive synthesis of beta-1,3-glucanase. It is concluded that the glucose repression effect on beta-1,3-glucanase production must be exerted at a pretranslational level that could be either mRNA synthesis or some stage of the process involved in its maturation or stabilization.
mesh_terms: D003513:Cycloheximide / Q000494:pharmacology; D004794:Enzyme Repression; D005656:Fungal Proteins / Q000096:biosynthesis; D004693:Glucan Endo-1,3-beta-D-Glucosidase / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology; D015125:Oxyquinoline / Q000494:pharmacology; D010407:Penicillium / Q000201:enzymology*; D012313:RNA / Q000096:biosynthesis
chemical_list: D005656:Fungal Proteins; D015125:Oxyquinoline; D012313:RNA; D003513:Cycloheximide; D004693:Glucan Endo-1,3-beta-D-Glucosidase; D005947:Glucose