Procedure for the isolation of mouse anti H-2 antibodies from complex alloantisera.
B6AF1-anti-B10.D2 ascites fluid was incubated with donor strain red blood cells to absorb the anti-H-2 antibodies. The antibody-coated RBC were then lysed, and from the ghosts the antibodies were eluted. This anti-H-2 preparation was further purified by affinity chromatography, using an immobilized anti IgG2 antiserum. Thus a pure anti-H-2 IgG2 was obtained for in vitro and in vivo testing.
D000818:Animals; D002846:Chromatography, Affinity; D003601:Cytotoxicity Tests, Immunologic; D004910:Erythrocyte Membrane / Q000276:immunology; D006649:Histocompatibility Antigens; D007106:Immune Sera; D005779:Immunodiffusion; D007074:Immunoglobulin G / Q000032:analysis; D007163:Immunosorbent Techniques; D007518:Isoantibodies / Q000302:isolation & purification*; D051379:Mice; D008805:Mice, Inbred A; D008810:Mice, Inbred C57BL
D006649:Histocompatibility Antigens; D007106:Immune Sera; D007074:Immunoglobulin G; D007518:Isoantibodies