Rapid, sensitive GLC determination of pentobarbital and other barbiturates in serum using nitrogen-specific detector.
A GLC method for the analysis of pentobarbital in serum was developed. After extraction from serum, a methyl derivative was prepared and quantitated by nitrogen-specific detection. The method has a senstivity of 0.08 microgram/ml for pentobarbital with only 0.1 ml of serum. Secobarbital was the internal standard. Derivatives of other barbiturates also were prepared.
D001463:Barbiturates / Q000097:blood*; D002849:Chromatography, Gas / Q000295:instrumentation; D006801:Humans; D008722:Methods; D010424:Pentobarbital / Q000097:blood*
D001463:Barbiturates; D010424:Pentobarbital