Fractionation and structure of several hydroxyproline-containing urinary peptides, with special reference to some 3-hydroxyproline-containing peptides.
After a preliminary separation of the hydroxyproline-containing peptides on Biogel P 2, the largest peptides are fractionated on phosphocellulose and the smallest ones on QAE-Sephadex. The fractions obtained from QAE-Sephadex are subfractionated on a column of Dowex 50-M-82. The total number of hydroxyproline-containing peptides from human urine is not less than 78. Sixteen di, tri and pentapeptides have been purified, their N-terminal amino acids and amino acid compositions determined and a structure is proposed. 3 of these peptides contain 3-hydroxyproline and one of these 3 peptides probably originates from basement membrane collagen.
D002852:Chromatography, Ion Exchange; D006801:Humans; D006909:Hydroxyproline / Q000032:analysis*; D010001:Osteitis Deformans / Q000652:urine; D010455:Peptides / Q000302:isolation & purification / Q000652:urine*
D010455:Peptides; D006909:Hydroxyproline