Direct assays of lactate, pyruvate, beta-hydroxybutyrate, and acetoacetate with a centrifugal analyzer.
Methods are described for direct assays of lactate, pyruvate, beta-hydroxybutyrate, and acetoacetate in plasma with the GEMSAEC centrifugal analyzer. The methods for lactate, beta-hydroxybutyrate, and acetoacetate are kinetic and ratiometric, eliminating the need for specimen-blank assays. The pyruvate method is an end-point assay, because endogenous lactate dehydrogenase interferes in a kinetic pyruvate assay. The methods are precise and accurate and 1-min of analysis time is adequate for each assay. Rapid assessment and monitoring of metabolic acidosis is possible with these methods, as is illustrated by examples.
D000090:Acetoacetates / Q000097:blood*; D000437:Alcoholism / Q000097:blood; D001322:Autoanalysis / Q000379:methods; D002498:Centrifugation; D016883:Diabetic Ketoacidosis / Q000097:blood / Q000188:drug therapy; D006801:Humans; D006885:Hydroxybutyrates / Q000097:blood*; D007328:Insulin / Q000627:therapeutic use; D007773:Lactates / Q000097:blood*; D011773:Pyruvates / Q000097:blood*; D012016:Reference Values
D000090:Acetoacetates; D006885:Hydroxybutyrates; D007328:Insulin; D007773:Lactates; D011773:Pyruvates