Selective tissue distribution of carbonic anhydrase isozymes in the ox.
By affinity chromatography the isozymic distribution of carbonic anhydrase (carbonate hydro-lyase, EC 4.2.1.1) has been studied in extract from various bovine tissues. Carbonic anhydrase II forms isolated from erythrocyte, kidney and brain are indistinguishable by specific activity, amino acid composition, fingerprint, electrophoretic and immunological behaviour. By these criteria they differ from carbonic anhydrase I isolated from rumen epithelium.
D000596:Amino Acids / Q000032:analysis; D000818:Animals; D001921:Brain / Q000201:enzymology*; D002256:Carbonic Anhydrases / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D002417:Cattle; D002846:Chromatography, Affinity; D004586:Electrophoresis; D004912:Erythrocytes / Q000201:enzymology*; D005779:Immunodiffusion; D007527:Isoenzymes; D007668:Kidney / Q000201:enzymology*; D012417:Rumen / Q000201:enzymology*
D000596:Amino Acids; D007527:Isoenzymes; D002256:Carbonic Anhydrases