A correlation between nucleosome spacer region susceptibility to DNase I and histone acetylation.
Hepatoma tissue culture (HTC) cell nuclei were digested with either DNase I or micrococcal nuclease and the nucleohistone digestion products fractionated by gel electrophoresis or exclusion chromatography. Under appropriate conditions, gel electrophoresis demonstrates that for both nucleases, only cleavages within the nucleosome spacer regions and not within the nucleosome core lead to freely migrating nucleohistone particles. These particles consist of nucleosome cores, nucleosomes and nucleosome oligomers. Following DNase I digestion and fractionation by exclusion chromatography, analysis of the histones indicates a direct relationship between increased spacer region susceptibility to nuclease and increased nucleosomal histone acetylation. Evidently digestion sites outside the regions of DNA protected by core histones can reflect the degree of acetylation of core histones. Such a relationship is not found when micrococcal nuclease is used to digest the samples.
D000107:Acetylation; D000818:Animals; D002460:Cell Line; D002843:Chromatin / Q000032:analysis; D004273:DNA, Neoplasm; D003851:Deoxyribonucleases; D006657:Histones; D008114:Liver Neoplasms, Experimental / Q000032:analysis; D008836:Micrococcal Nuclease; D051381:Rats
D002843:Chromatin; D004273:DNA, Neoplasm; D006657:Histones; D003851:Deoxyribonucleases; D008836:Micrococcal Nuclease