Cytidine 5'-diphosphate reductase activity in phytohemagglutinin stimulated human lymphocytes.
The optimal conditions and the effect of deoxyribonucleoside triphosphates were determined for CDP reductase activity in PHA-stimulated lymphocytes. The enzymatic reaction showed an absolute requirement for ATP. In the absence of ATP, only dATP showed a minor stimulation of the reduction of CDP to dCDP. During transformation the CDP reductase activity reached a maximum at the same time as the four deoxyribonucleoside triphosphate pools, corresponding to mid S-phase at about 50 h after PHA addition. The DNA polymerase activity reached a maximum at 57 h.
D000255:Adenosine Triphosphate / Q000494:pharmacology; D003565:Cytidine Diphosphate; D004247:DNA / Q000096:biosynthesis; D003854:Deoxyribonucleotides / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D007700:Kinetics; D037102:Lectins; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000378:metabolism*; D008274:Magnesium / Q000494:pharmacology; D012262:Ribonucleoside Diphosphate Reductase / Q000378:metabolism*; D012264:Ribonucleotide Reductases / Q000378:metabolism*
D003854:Deoxyribonucleotides; D037102:Lectins; D003565:Cytidine Diphosphate; D000255:Adenosine Triphosphate; D004247:DNA; C019857:CDP reductase; D012264:Ribonucleotide Reductases; D012262:Ribonucleoside Diphosphate Reductase; D008274:Magnesium