Effects of hemorrhagic shock on nuclear DNA.
Rats, with their liver DNA labeled with 3H-thymidine, were subjected to hemorrhagic shock. In nine rats reversible hemorrhagic shock with a blood pressure of 40 mm Hg for 30 minutes resulted in no breaks in the DNA. Twenty-seven animals were subjected to severe hemorrhagic shock. Blood pressures of 40 mm Hg were maintained in the 27 animals until 50% of the shed blood was infused in 11 animals and until 70% of the shed blood was reinfused in 16 animals. This degree of hemorrhage resulted in the DNA being broken, so that 45.1% and 52.1% of the DNA was of a small molecular weight species, as compared with 9.5% and 10.8% in the preshocked animals (P less than 0.001). The injured DNA was not repaired 1.5 hours after the animals were resuscitated in the animals subjected to severe hemorrhagic shock.
D000818:Animals; D001803:Blood Transfusion; D002467:Cell Nucleus / Q000378:metabolism*; D002499:Centrifugation, Density Gradient; D004247:DNA / Q000032:analysis / Q000096:biosynthesis; D008099:Liver / Q000032:analysis / Q000378:metabolism; D008970:Molecular Weight; D051381:Rats; D012771:Shock, Hemorrhagic / Q000378:metabolism*
D004247:DNA