[Observations about the use of different types of enzymes in immunohistological diagnosis of autoimmune diseases. Comparative study with indirect immunofluorescence technique (author's transl)].
The Authors have evaluated the use of anti-human immunoglobulins conjugated with peroxidase, alkaline-phosphatase, glucose-oxidase as a diagnostic tool for assaying autoantibodies. A comparison study was done using the classical indirect immunofluorescence technique. Immunoenzymatic techniques, of minor cost compared to immunofluorescence, have revealed a better appreciation of global and particular structure of the tissue in examination. From the three enzymes examined peroxidase revealed itself as first choice, because, other than having on overlapping sensitivity to immunofluorescence, it can allow a further ultrastructural study.
D000469:Alkaline Phosphatase; D000818:Animals; D001323:Autoantibodies / Q000032:analysis*; D001327:Autoimmune Diseases / Q000175:diagnosis*; D005069:Evaluation Studies as Topic; D005455:Fluorescent Antibody Technique; D005949:Glucose Oxidase; D006801:Humans; D007124:Immunoenzyme Techniques; D010544:Peroxidases; D051381:Rats
D001323:Autoantibodies; D005949:Glucose Oxidase; D010544:Peroxidases; D000469:Alkaline Phosphatase
Osservazioni sull'impiego di differenti tipi di enzimi nella diagnostica immunoistologica delle malattie autoimmuni. Valutazione comparativa con la tecnica dell'immunofluorescenza indiretta.