Studies on the chemical composition of lipopolysaccharide from Neisseria meningitidis group B.
A lipopolysaccharide was isolated from Neisseria meningitidis group B by phenol/water extraction and purified by differential ultracentrifugation. This preparation exhibited endotoxic properties as shown by the limulus-lysate assay. Mild acid hydrolysis of the lipopolysaccharides yielded a lipid A fraction and a polysaccharide fraction. The lipid A fraction contained fatty acids, phosphorus and glucosamine. Analysis of the polysaccharide fraction revealed the presence of glucose, galactose, glucosamine, 2-keto-3-deoxyoctonic acid and phosphorus. There was no heptose.
D005227:Fatty Acids / Q000032:analysis; D008050:Lipid A / Q000032:analysis; D008070:Lipopolysaccharides / Q000032:analysis*; D009005:Monosaccharides / Q000032:analysis; D009345:Neisseria meningitidis / Q000032:analysis*
D005227:Fatty Acids; D008050:Lipid A; D008070:Lipopolysaccharides; D009005:Monosaccharides