Growth of an enveloped mycoplasmavirus and establishment of a carrier state.
The growth of an enveloped DNA-containing mycoplasmavirus (MVL2 obtained from R.N. Gourlay) has studied, by using the indicator host Acholeplasma laidlawii strain JA1. From virus one-step growth curves, artificial lysis experiments, and infected cell growth curves, it was found that virus infection is nonlytic. Newly infected cells grow slower and are osmotically more stable than uninfected cells. However, 4 to 6 h after infection, the cells reach a carrier state in which cell growth rate and osmotic fragility are indistinguishable from uninfected cells. Carrier cultures contain free virus. Every carrier culture cell gives rise to either a clone of carrier cells or a clone of MVL2-resistant cells.
D000128:Acholeplasma laidlawii / Q000187:drug effects / Q000254:growth & development; D000327:Adsorption; D001435:Bacteriophages / Q000254:growth & development*; D002455:Cell Division; D004492:Edetic Acid / Q000494:pharmacology; D009996:Osmotic Fragility; D014325:Tromethamine / Q000494:pharmacology; D014779:Virus Replication
D014325:Tromethamine; D004492:Edetic Acid