A new site-specific endonuclease from Streptomyces lavendulae (SlaI).
A new restriction-like endonuclease, SlaI, was found and partially purified from Streptomyces lavendulae ATCC8664. This endonuclease cleaved bacteriophage lambda DNA at only one site, and cytosine-substituted bacteriophage T4 DNA at 16 sites. The recognition sequence was determined by using SlaI fragments of cytosine-substituted bacteriophage T4 DNA. The hexanucleotide recognized by SlaI endonuclease was (Formula: see text), with the sites of cleavage as indicated by the arrows. Therefore, SlaI endonuclease was an isoschizomer of XhoI endonuclease.
D001483:Base Sequence; D003090:Coliphages / Q000235:genetics; D003596:Cytosine / Q000378:metabolism; D004262:DNA Restriction Enzymes / Q000302:isolation & purification*; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D011995:Recombination, Genetic; D013302:Streptomyces / Q000201:enzymology* / Q000235:genetics
D004279:DNA, Viral; D003596:Cytosine; D004262:DNA Restriction Enzymes