[beta-Glucosidases specific for the cyanogenic glucoside triglochinin from Alocasia macrorrhiza Schott: further characterization of properties (author's transl)].
All beta-glucosidases extracted and separated from a plant of Alocasia macrorrhiza are almost entirely specific for triglochinin. The hexameric beta-glucosidase has been shown to dissociate in dimers without any alteration of activity. Reaggregation could only be demonstrated using bifunctional reagents like glutaraldehyde. Treatments of beta-glucosidase with various chemicals (e. g. glutaraldehyde, dodecyl sulfate) decreased the activity for triglochinin more than the activity for 4-nitrophenyl glucoside. On the other hand, specific reagents like bromocondurite or p-chloromercuribenzoate caused identical inactivations measured with various substrates. It seems possible that the different beta-glucosidases splitting triglochinin arose during purification from the hexameric form which occurs in the plant.
D000272:Adipates; D005959:Glucosidases / Q000378:metabolism; D005960:Glucosides; D005976:Glutaral; D006027:Glycosides; D046911:Macromolecular Substances; D009570:Nitriles; D010944:Plants / Q000201:enzymology*; D013379:Substrate Specificity; D001617:beta-Glucosidase / Q000302:isolation & purification / Q000378:metabolism
D000272:Adipates; D005960:Glucosides; D006027:Glycosides; D046911:Macromolecular Substances; D009570:Nitriles; C015557:triglochinin; D005959:Glucosidases; D001617:beta-Glucosidase; D005976:Glutaral
Spezifische beta-Glucosidasen für das cyanogene Glucosid Triglochinin aus Alocasia macrorrhiza Schott Weitere Charakterisierung der Eigenschaften.