title: Superiority of a soil debris isolation method over a beet seed colonization method for assay of Rhizoctonia solani at high soil inoculum densities.
abstract: A quantitative soil debris isolation method (all debris from known weight of soil plated) and a garden beet seed saprophytic colonization method were compared over a 1-year period for assaying Rhizoctonia solani population. Four fields of different soil textures were selected. Within each field four areas of healthy and four areas of diseases (rhizoctonia root and crown rot) sugarbeets were sampled bimonthly from August 1976 until June 1977. The maximum numbers of R. solani colonies obtained by the debris method were 2 per gram of soil in areas of healthy beets, and 11 per gram of soil in areas of diseased sugarbeets. At such high inoculum densities the beet seed colonization method underestimated R. solani populations, because the inoculum per unit of soil exceeded the numbers of beet seeds per unit of soil available for colonization. Modifications of the beet seed method did not significantly alter results of colonization assays. Ranked correlation comparisons of assay methods yielded r = 0.81 for all data.
mesh_terms: D003904:Mitosporic Fungi / Q000254:growth & development*; D012232:Rhizoctonia / Q000254:growth & development* / Q000302:isolation & purification; D012988:Soil Microbiology