An examination of complement proteins on membranes of paroxysmal nocturnal haemoglobinuria (PNH) and PNH-like red cells.
Proteins obtained from membranes of PNH erythrocytes and of red cells treated with 2-aminoethylisothiouronium bromide (AET) following lysis in acidified serum (Ham's test) were compared by sodium dodecylsulphate-polyacrylamide gel electrophoresis. The peptide patterns were identical, providing support that AET-treated red cells afford a suitable experimental substitute for PNH red cells in studies involving complement fixation.
D001797:Blood Protein Electrophoresis; D003165:Complement System Proteins; D004910:Erythrocyte Membrane / Q000032:analysis*; D004912:Erythrocytes / Q000032:analysis*; D006457:Hemoglobinuria, Paroxysmal / Q000097:blood*; D006801:Humans; D008565:Membrane Proteins / Q000032:analysis*; D008970:Molecular Weight; D010455:Peptides / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D000338:beta-Aminoethyl Isothiourea / Q000494:pharmacology
D008565:Membrane Proteins; D010455:Peptides; D011506:Proteins; D000338:beta-Aminoethyl Isothiourea; D003165:Complement System Proteins