Plaque assay of baculoviruses employing an agarose-nutrient overlay.
Four baculoviruses produced visible plaques in the Spodoptera frugiperda cell line when cell monolayers were infected and overlayed with a simplified, agarose-nutrient formulation. Macroscopic plaques were first detected 4 days postinoculation, and by 10 days plaques ranging from 0.5 to 3 mm in diameter were seen. Dose-response experiments indicated that a single particle initiated the formation of a plaque, because a linear response was demonstrated with increased dosage.
D000818:Animals; D002460:Cell Line; D004267:DNA Viruses; D007181:Inclusion Bodies, Viral; D007304:Insect Viruses / Q000254:growth & development*; D007313:Insecta; D012685:Sepharose; D010948:Viral Plaque Assay / Q000379:methods*
D012685:Sepharose