The respiratory chain of a newly isolated Methylomonas Pl1.
1. Whole cells of Methylomonas Pl1 contained ubiquinone, identified as ubiquinone-8. No naphthaquinone was detected. Ubiquinone was located predominantly in the particulate fraction, which also contained most of the NADH oxidase activity. 2. Aerobic incubation of cells with formaldehyde or methanol resulted in about 20% reduction of ubiquinone, irrespective of the presence or absence of dinitrophenol. On inhibition of the respiration by cyanide, ubiquinone became partly reduced by endogenous substrates (15--25%), and a further reduction occurred only in the presence of formaldehyde (up to 60%). When endogenous substrates were completely exhausted, then 44 and 23% of ubiquinone was reduced by formaldehyde or methanol respectively. 3. The difference spectra at room and liquid-N2 temperatures revealed the presence of cytochrome b and two cytochromes c (c-552.5 and c-549) all tightly bound to the membrane. Cytochrome c-552.5 was also found in the soluble fraction. 4. Redox changes of cytochromes b and c, with methanol or formaldehyde as substrates, respond to the aerobic and anaerobic states of the cell and to KCN inhibition in a manner characteristic of the electron carriers of the respiratory chain. 5. The merging point for electron transport from NADH dehydrogenase and formaldehyde dehydrogenase is suggested to be at the level of ubiquinone.
D002855:Chromatography, Thin Layer; D003580:Cytochromes / Q000378:metabolism; D005557:Formaldehyde / Q000378:metabolism; D000432:Methanol / Q000378:metabolism; D008772:Methylococcaceae / Q000378:metabolism*; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D011809:Quinones / Q000378:metabolism; D013057:Spectrum Analysis; D014451:Ubiquinone / Q000378:metabolism
D003580:Cytochromes; D011809:Quinones; D014451:Ubiquinone; D005557:Formaldehyde; D000432:Methanol