Mutants of Bacillus megaterium resistant to uncouplers of oxidative phosphorylation.
Mutants of Bacillus megaterium displaying malate-stimulated ATP synthesis resistant to uncouplers of oxidative phosphorylation were isolated and partially characterized. ATP synthesis in such mutants was resistant to carbonyl cyanide m-chlorophenyl hydrazone as well as to other uncouplers including 2,4-dinitrophenol, pentachlorophenol, and sodium azide. ATP synthesis in the wild type and in resistant mutants was sensitive to N,N'-dicyclohexylcarbodiimide, tributyltin, valinomycin plus potassium, and potassium cyanide. Active transport of glycine and glutamine which are sensitive to uncouplers in the wild type was also uncoupler-sensitive in the mutants.
D000255:Adenosine Triphosphate / Q000378:metabolism; D001410:Bacillus megaterium / Q000187:drug effects / Q000378:metabolism*; D001693:Biological Transport, Active; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D004352:Drug Resistance, Microbial; D005973:Glutamine / Q000378:metabolism; D005998:Glycine / Q000378:metabolism; D007700:Kinetics; D008293:Malates / Q000378:metabolism; D009154:Mutation; D010085:Oxidative Phosphorylation / Q000187:drug effects*; D014475:Uncoupling Agents / Q000494:pharmacology*
D008293:Malates; D014475:Uncoupling Agents; D005973:Glutamine; D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D000255:Adenosine Triphosphate; D005998:Glycine