Cell cycle regulation in mammalian cells: hormones and commitment to DNA synthesis.
The growth regulation of cultured mouse fibroblasts and functional adrenal cells was studied. Variants of mutants from these cell lines were obtained. The effects of classical hormones (insulin, hydrocortisone and adrenocorticotropin) and of growth factors (EGF and PF) were analysed. These hormones stimulate or inhibit the entry of cells into S phase. However G1 cells become irreversibly committed to DNA synthesis 5 hours before entering S phase.
D000311:Adrenal Glands / Q000166:cytology; D000818:Animals; D002453:Cell Cycle / Q000187:drug effects*; D002478:Cells, Cultured; D004261:DNA Replication / Q000187:drug effects*; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005347:Fibroblasts; D006133:Growth Substances / Q000494:pharmacology*; D006728:Hormones / Q000494:pharmacology*; D006854:Hydrocortisone / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D007399:Interphase / Q000187:drug effects; D051379:Mice; D009154:Mutation; D011460:Prostaglandins F / Q000494:pharmacology; D013002:Somatomedins / Q000494:pharmacology; D013997:Time Factors
D006133:Growth Substances; D006728:Hormones; D007328:Insulin; D011460:Prostaglandins F; D013002:Somatomedins; D004815:Epidermal Growth Factor; D006854:Hydrocortisone