Complete sharing of light chain spectrotypes by murine IgM and IgG anti-streptococcal antibodies.
In order to examine the diversity of antibody light chains, we have developed an analytic isoelectric focusing procedure which permits the routine analysis of L chains from antibodies raised in individual mice. We have used this technique to demonstrate that the light chains of IgM and IgG anti-group A streptococcal antibodies raised in SWR mice are probably shared. Interestingly, numerous light chain spectrotypes are shared between individual mice whose 7S antibody focusing patterns differ.
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D007106:Immune Sera / Q000032:analysis; D007074:Immunoglobulin G; D007147:Immunoglobulin Light Chains / Q000032:analysis*; D007075:Immunoglobulin M; D007525:Isoelectric Focusing; D051379:Mice; D008807:Mice, Inbred BALB C; D013297:Streptococcus pyogenes / Q000276:immunology*
D000907:Antibodies, Bacterial; D007106:Immune Sera; D007074:Immunoglobulin G; D007147:Immunoglobulin Light Chains; D007075:Immunoglobulin M