title: Purified human liver acid beta-D-galactosidases possessing activity towards G(M1)-ganglioside and lactosylceramide.
abstract: Our studies with purified human liver acid beta-D-galactosidases (EC 3.2.1.23) indicate that 4-methylumbelliferyl beta-D-galactosidase and G(M1)-ganglioside beta-D-galactosidase activities are identical with lactosylceramidase II activity. Evidence for this includes co-purification of all enzyme activities by affinity chromatography to yield a single band on polyacrylamide-gel electrophoresis and coincident elution from Sepharose 6B of all three enzyme activities.
mesh_terms: D002554:Cerebrosides; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D005677:G(M1) Ganglioside; D005696:Galactosidases / Q000378:metabolism*; D006801:Humans; D007785:Lactose / Q000031:analogs & derivatives; D008099:Liver / Q000201:enzymology; D001616:beta-Galactosidase / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D002554:Cerebrosides; D005677:G(M1) Ganglioside; D005696:Galactosidases; D001616:beta-Galactosidase; D007785:Lactose