Purification of human monocytes on microexudate-coated surfaces.
Human blood monocytes, but not other Ficoll-Hypaque cells, adhere to plastic surfaces from which confluent BHK cells have been removed. EDTA (3mM) rapidly and completely removes monocytes, providing a simple technique for preparation of human monocytes in suspension. The method should be valuable for studies of monocyte function where monocyte monolayers are not suitable.
D000818:Animals; D002448:Cell Adhesion; D002469:Cell Separation; D006224:Cricetinae; D004492:Edetic Acid / Q000494:pharmacology; D005362:Ficoll; D006801:Humans; D051379:Mice; D009000:Monocytes
D005362:Ficoll; D004492:Edetic Acid