Iodination of Escherichia coli with chloramine T: selective labeling of the outer membrane lipoprotein.
Iodination of Escherichia coli cells with chloramine T preferentially labels the free and murein-bound forms of the outer membrane lipoprotein. Iodination for 15 s at 15 degrees C labels the two forms of the lipoprotein almost exclusively, whereas iodination for 60 s at 25 degrees C also labels the other major outer membrane proteins. Chloramine T iodination is a rapid, simple technique for labeling the outer membrane lipoprotein.
D001120:Arginine / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D011232:Chemical Precipitation; D002700:Chloramines / Q000494:pharmacology*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000378:metabolism*; D007455:Iodine / Q000378:metabolism*; D007457:Iodine Radioisotopes; D008074:Lipoproteins / Q000276:immunology / Q000378:metabolism*; D008970:Molecular Weight; D010457:Peptidoglycan / Q000378:metabolism; D014105:Tosyl Compounds; D014443:Tyrosine / Q000378:metabolism
D001425:Bacterial Outer Membrane Proteins; D002700:Chloramines; D007457:Iodine Radioisotopes; D008074:Lipoproteins; D010457:Peptidoglycan; D014105:Tosyl Compounds; C016300:chloramine-T; D014443:Tyrosine; D001120:Arginine; D007455:Iodine