title: Permeability of Ehrlich ascites cells and spectrophotometric assay of the sub-cellular enzymes.
abstract: A permeabilization method which allows the assay of intracellular enzymes of the Ehrlich ascites cells is described. The developmental changes in the activity of lactic dehydrogenase and glucose-6-phosphate dehydrogenase of toluene-treated Ehrlich ascites cells were studied.
mesh_terms: D000818:Animals; D002286:Carcinoma, Ehrlich Tumor / Q000201:enzymology*; D002463:Cell Membrane Permeability / Q000187:drug effects; D002478:Cells, Cultured / Q000187:drug effects* / Q000201:enzymology; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D051379:Mice; D014050:Toluene / Q000494:pharmacology*
chemical_list: D014050:Toluene; D007770:L-Lactate Dehydrogenase; D005954:Glucosephosphate Dehydrogenase