[In vitro malignant transformation of fetal hamster brain cells by methylnitrosourea].
Using primary cultures originating from 14 day old fetal Hamster brain, we have obtained a cell line with glial morphology. These cell remain non transplantable during the first year of in vitro culture, but undergo spontaneous transformation during the second year. Following prolonged contact of the glial-like  cells with 25 to 50 microgram/ml of methylnitrosourea (MNU), a malignant transformation is observed 5 months after the treatment. The lowest concentrations of (MNU) do not cause malignant transformation, but seem to inhibit (or postpone) the spontaneous transformation. After MNU treatment, cells retain their glial nature.
D000818:Animals; D001921:Brain / Q000187:drug effects*; D001932:Brain Neoplasms / Q000139:chemically induced; D002471:Cell Transformation, Neoplastic; D006224:Cricetinae; D008770:Methylnitrosourea / Q000494:pharmacology*; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental; D009457:Neuroglia / Q000187:drug effects; D009607:Nitrosourea Compounds / Q000494:pharmacology*
D009607:Nitrosourea Compounds; D008770:Methylnitrosourea
Transformation maligne in vitro de cellules de cerveau faetal de hamster par la méthylnitrosourée.