Specific cytosol receptors for 1,25-dihydroxyvitamin D3 in human intestine.
Human intestine is shown to contain a specific, high affinity cytosol receptor or binding protein for 1,25-(OH)2-vitamin D3 [1,25 (OH)2D3]. This receptor is a protein which sediments at 3.5S on sucrose gradients containing 0.3 M KCL. The receptor binds 1,25 (OH)2D3 with a Kd of approximately 2 x 10(-10) M at 4 degrees C. Competition binding studies using structural analogs of 1,25 (OH)2D3 indicate that the relative specificity of the receptor is 1,25 (OH)2D3 greater than 1,24R,25(OH)3D3 greater than greater than 25OHD3 1 OHD3 greater than 24R,25(OH)2D3.
D000328:Adult; D001667:Binding, Competitive; D003600:Cytosol / Q000378:metabolism; D004100:Dihydroxycholecalciferols / Q000378:metabolism*; D006801:Humans; D006887:Hydroxycholecalciferols / Q000378:metabolism*; D007583:Jejunum / Q000378:metabolism*; D007700:Kinetics; D011955:Receptors, Drug / Q000302:isolation & purification / Q000378:metabolism*
D004100:Dihydroxycholecalciferols; D006887:Hydroxycholecalciferols; D011955:Receptors, Drug