title: Two-dimensional restriction analysis of the Bacillus subtilis genome: gene purification and ribosomal ribonucleic acid gene organization.
abstract: With two-dimensional restriction enzyme analysis we have been able to cleave the Bacillus subtilis genome and resolve the resulting deoxyribonucleic acid (DNA) segments into discrete bands on agarose gels. A general procedure for gene purification has been developed by coupling multidimensional restriction analysis with a biological assay for gene detection. The organization of ribosomal ribonucleic acid (rRNA) genes was studied by hybridizing 16S and 23S rRNA probes to the two-dimensional DNA banding patterns.
mesh_terms: D001412:Bacillus subtilis / Q000032:analysis / Q000378:metabolism; D004262:DNA Restriction Enzymes; D004269:DNA, Bacterial / Q000032:analysis*; D005796:Genes; D008722:Methods; D012335:RNA, Ribosomal / Q000096:biosynthesis*
chemical_list: D004269:DNA, Bacterial; D012335:RNA, Ribosomal; D004262:DNA Restriction Enzymes