Activity of some enzymes during growth and sporulation of Clostridium botulinum type E.
The activities of alkaline and acid phosphatases, glucose dehydrogenase and NADH oxidase were assayed in cell-free extracts of sporogenic and asporogenic mutants of Clostridium botulinum. During growth of both mutants, the activities of alkaline and acid phosphatases were relatively constant, but during sporulation of the sporogenic mutant, the alkaline phosphatase activity rose to a maximum of 70 mumol/min x mg protein whereas the acid phosphatase decreased rapidly before it increased, indicating a possible role in sporogenesis. Glucose dehydrogenase activity was detected only in cell-free extracts of the sporogenic mutant and reached a maximum of 7 mumol/min x mg protein during the endospore maturation stage. The NADH oxidase activity was detected in both mutants. The NADH oxidase seems to stimulate glucose oxidation in both mutants during growth and the dehydrogenation processes of the butyric type of fermentation during spore formation in the sporogenic mutant. The findings suggest that increased glucose dehydrogenase activity in C. botulinum, as in Bacillus species, may serve as a spore event marker and that alkaline and acid phosphatases may play a regulatory role in anaerobic sporulation metabolism.
D000135:Acid Phosphatase / Q000378:metabolism*; D000469:Alkaline Phosphatase / Q000378:metabolism*; D001905:Botulinum Toxins / Q000235:genetics; D002237:Carbohydrate Dehydrogenases / Q000378:metabolism*; D003014:Clostridium botulinum / Q000201:enzymology / Q000235:genetics / Q000254:growth & development*; D005948:Glucose Dehydrogenases / Q000378:metabolism*; D009154:Mutation; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D013172:Spores, Fungal
D002237:Carbohydrate Dehydrogenases; D005948:Glucose Dehydrogenases; D009247:NADH, NADPH Oxidoreductases; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D001905:Botulinum Toxins