title: Improved procedure of purification of inorganic pyrophosphatase from Bacillus stearothermophilus.
abstract: An improved method for isolation of inorganic pyrophosphatase (EC 3.6.1.1) from Bacillus stearothermophilus is described. The enzyme was purified to more than 90% after two chromatographic steps. A molecular weight of 140,000 daltons was estimated by density gradient centrifugation. The isoelectric point was found to be 4.0.
mesh_terms: D002499:Centrifugation, Density Gradient; D002845:Chromatography; D001411:Geobacillus stearothermophilus / Q000201:enzymology*; D008970:Molecular Weight; D011755:Pyrophosphatases / Q000302:isolation & purification*
chemical_list: D011755:Pyrophosphatases