End-point dilution and plaque assay methods for titration of cricket paralysis virus in cultured Drosophila cells.
Cricket paralysis virus, an insect picorna-like virus, caused a distinct c.p.e. in cultured Drosophila melanogaster cells, allowing the development of titration methods based on end-point dilution or plaque assay methods. The end-point dilution (TCD50) method is more sensitive and economical than plaque assays and is easily scored. The data indicate a minimum infectivity/particle ratio of about 1/2000.
D046508:Culture Techniques; D003588:Cytopathogenic Effect, Viral; D004331:Drosophila melanogaster; D005069:Evaluation Studies as Topic; D007304:Insect Viruses / Q000254:growth & development / Q000302:isolation & purification*; D008722:Methods; D010948:Viral Plaque Assay; D014776:Virus Cultivation; D014779:Virus Replication