[The effects of 4 detergents on the respiration of mitochondria isolated from rat liver].
The effects of these four detergents (two non-ionic: Titron X100 and Tween 80, two ionic: sodium cholate and sodium dedeoxycholate) upon the respiratory intensities of mitochondria and upon the ADP/0 and respiratory control ratios were observed. At low concentrations and in the absence of exogenous ADP, non ionic as well as ionic detergents provoked a threefold (or fourfold) increase of the respiratory intensities of mitochondria. At higher concentrations, the four detergents were inhibitory for mitochondrial oxidations in the order: Triton X 100 greater than DOC greater than cholate greater than Tween 80. At increasing doses, the four detergents progressively decreased the phosphorylating capacities of mitochondria.
D000244:Adenosine Diphosphate / Q000494:pharmacology; D000818:Animals; D002474:Cell-Free System; D002793:Cholic Acids / Q000494:pharmacology*; D003840:Deoxycholic Acid / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D008297:Male; D008930:Mitochondria, Liver / Q000187:drug effects*; D010085:Oxidative Phosphorylation / Q000187:drug effects*; D010101:Oxygen Consumption / Q000187:drug effects*; D011092:Polyethylene Glycols / Q000494:pharmacology*; D011136:Polysorbates / Q000494:pharmacology*; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology*; D051381:Rats
D002793:Cholic Acids; D011136:Polysorbates; D000644:Quaternary Ammonium Compounds; D003840:Deoxycholic Acid; D011092:Polyethylene Glycols; D000244:Adenosine Diphosphate
Effets de quatre détergents sur la respiration de mitochondries isolées du foie de rat