Determination of guanfacine in biological fluids by electron-capture GLC.
A quantitative electron-capture GLC method with good sensitivity is described for the determination of guanfacine in human plasma and urine. By condensing its amidino group with hexafluoroacetylacetone, guanfacine is converted to a pyrimidino derivative with better GLC properties than the parent drug. This method allows the determination of guanfacine in plasma and urine at concentrations as low as 0.5 ng/ml and was applied successfully to measurement of plasma levels in humans after therapeutic dosing.
D000959:Antihypertensive Agents / Q000032:analysis* / Q000097:blood; D001769:Blood; D002849:Chromatography, Gas; D006146:Guanidines / Q000032:analysis*; D006801:Humans; D007700:Kinetics; D008722:Methods; D010648:Phenylacetates / Q000032:analysis* / Q000097:blood
D000959:Antihypertensive Agents; D006146:Guanidines; D010648:Phenylacetates