Effects of phenylmethylsulphonylfluoride on activities of cholesteryl ester synthesis and hydrolysis in testes of rats, and on serum testosterone and LH levels.
PMSF was injected subcutaneously to male rats once a day for 10 days at a dose of 10 mg/day. There was an increase in the concentrations of free and esterified cholesterol in the testes, a decrease in activities of cholesteryl ester synthesis and hydrolysis but no change in activity of cholesterol side-chain cleavage enzyme. Serum testosterone and LH levels were significantly decreased. The only effect of PMSF on the lipids of the testes was a marked elevation of docosapentaenoic (22:5) acid and the cholesteryl esters.
D000818:Animals; D002784:Cholesterol / Q000096:biosynthesis; D002788:Cholesterol Esters / Q000096:biosynthesis*; D006868:Hydrolysis; D007986:Luteinizing Hormone / Q000097:blood*; D008297:Male; D010664:Phenylmethylsulfonyl Fluoride / Q000494:pharmacology*; D051381:Rats; D013450:Sulfones / Q000494:pharmacology*; D013737:Testis / Q000187:drug effects / Q000378:metabolism*; D013739:Testosterone / Q000097:blood*
D002788:Cholesterol Esters; D013450:Sulfones; D013739:Testosterone; D010664:Phenylmethylsulfonyl Fluoride; D007986:Luteinizing Hormone; D002784:Cholesterol