Reproducible chromosome changes of polycyclic hydrocarbon-induced rat leukemia: incidence and chromosome banding pattern.
Statistical analysis of 361 cases of primary leukemia induced in outbred Long-Evans and Sprague-Dawley rats by 7,12-dimethylbenz[a]anthracene (DMBA) and 7,8,12-trimethylbenz[a]anthracene (TMBA) showed that the incidence of trisomy of chromosome No. 2 was significantly lower with TMBA (17.8%) than with DMBA (29.3%). This tendency was reproducible in both sexes. Another characteristic chromosome abnormality, long No. 2, was found in 10 cases (2.8%). Quinacrine fluorescence analysis revealed that cells with No. 2 trisomy or either of two types of long No. 2 had total and partial No. 2 trisomy, respectively. Other chromosome members of cells with long No. 2, as well as the chromosomes of cells with typical No. 2 trisomy and "normal diploid" leukemia cells, revealed no band abnormality. The phenotype of No. 2 trisomy, severe anemia of the hosts reported in DMBA-induced leukemias, was also noted in leukemias with TMBA-induced No. 2 trisomy but not in leukemias with long No. 2.
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000031:analogs & derivatives; D000740:Anemia / Q000150:complications / Q000235:genetics; D000818:Animals; D001551:Benz(a)Anthracenes / Q000031:analogs & derivatives; D002869:Chromosome Aberrations; D005260:Female; D005453:Fluorescence; D007942:Leukemia, Experimental / Q000139:chemically induced / Q000150:complications / Q000235:genetics*; D008297:Male; D011796:Quinacrine; D051381:Rats; D014314:Trisomy
D001551:Benz(a)Anthracenes; D015127:9,10-Dimethyl-1,2-benzanthracene; D011796:Quinacrine