Nuclear magnetic relaxation of 19F as a novel assay method of superoxide dismutase.
The 19F longitudinal nuclear magnetic relaxation rate (T-1(1p)) of F- solutions is greatly enhanced by copper, zinc or manganese superoxide dismutase. The measured T-1(1p) values are at least an order of magnitude, and in most cases 2 orders of magnitude, higher than those of other metal-containing proteins and low molecular weight complexes. This property is suitable for a direct, specific, and sensitive assay of superoxide dismutase, free of interference by other molecules. For mixtures of the copper, zinc and manganese enzymes, addition of CN- permits quantification of the relative amounts of each enzyme. In the case of copper, zinc enzyme, the method proved exquisitely sensitive to the native state of the active site. Results are reported to show that the 19F relaxation method can be used to assay biological fluids and crude homogenates for copper, zinc and manganese superoxide dismutases, under conditions that offer considerable advantages in comparison to other assay methods.
D000818:Animals; D001921:Brain / Q000201:enzymology; D002417:Cattle; D003300:Copper; D005461:Fluorine; D008099:Liver / Q000201:enzymology; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008345:Manganese; D008667:Metalloproteins / Q000032:analysis; D013482:Superoxide Dismutase / Q000032:analysis* / Q000097:blood; D015032:Zinc
D008667:Metalloproteins; D005461:Fluorine; D008345:Manganese; D003300:Copper; D013482:Superoxide Dismutase; D015032:Zinc