title: DNA polymerases of anucleated cells. Isolation and characterization of two DNA polymerases from human platelets.
abstract: Two different DNA polymerases have been purified and characterized from human platelets. In the mitochondrial fraction a unique activity of the polymerase gamma type has been found. The same enzyme is found in the extramitochondrial supernatant. A second DNA polymerase, called 'cytoplasmic' DNA polymerase has been found in the 10000 x g supernatant of human platelets. The following properties of the latter DNA polymerase from human platelets are identical to those of DNA polymerase alpha from normal cells: DEAE-cellulose and phosphocellulose chromatography, size, thermal stability, phosphonoacetic acid and ethidium bromide inhibition. However, some of its properties, like high resistance to N-ethylmaleimide and the lack of DNA polymerization using synthetic RNA primers, are those of DNA polymerase beta.
mesh_terms: D001792:Blood Platelets / Q000201:enzymology*; D003593:Cytoplasm / Q000201:enzymology; D004258:DNA Polymerase III / Q000097:blood; D004259:DNA-Directed DNA Polymerase / Q000097:blood* / Q000302:isolation & purification / Q000378:metabolism; D006367:HeLa Cells / Q000201:enzymology; D006801:Humans; D007700:Kinetics
chemical_list: D004258:DNA Polymerase III; D004259:DNA-Directed DNA Polymerase