Comparison of four culture media for isolation of Mycobacterium avium complex from porcine tissues.
The efficiency of four culture media was compared for the isolation of Mycobacterium avium complex from 197 procine tissues. In 82 tissues with microscopic granulomas and acid-fast bacilli, a significantly greater number of isolates were obtained on Middlebrook 7H10 medium with sodium pyruvate than on Stonebrink medium, Herrold egg yolk agar medium, or Lowenstein-Jensen medium (P=0.01). In 46 tissues in which no microscopic granulomas or acid-fast bacilli were observed, a significantly greater number of isolates were made on Middlebrook 7H10 medium or Herrold egg yolk agar medium than on Stonebrink medium or on Lowenstein-Jensen medium (P=0.01). The time required to grow M. avium complex on Lowenstein-Jensen medium was significantly greater than the time required to observe growth on Stonebrink, Middlebrook 7H10, or Herrold egg yolk agar medium (p=0.001).
D000818:Animals; D003470:Culture Media; D008198:Lymph Nodes / Q000382:microbiology; D009161:Mycobacterium / Q000302:isolation & purification*; D009162:Mycobacterium avium / Q000145:classification / Q000302:isolation & purification*; D012703:Serotyping; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*; D014376:Tuberculosis / Q000382:microbiology / Q000662:veterinary*
D003470:Culture Media