title: Influence of age and spironolactone on lysosomal enzyme activities, DNA and protein content of the rat liver after partial hepatectomy.
abstract: Measurements of DNA and protein content and determinations of lysosomal enzyme activities (beta-glucuronidase, beta-acetylglucosaminidase, cathepsin D) were performed in the rat liver following partial hepatectomy and spironolactone administration. The rats (SIV) were divided into three age-groups: 6 weeks old, 10 months old and 18 months old. 60 h after the administration of spironolactone (2 x 10 mg Aldactone/100 g body weight/day orally via gastric tube for 3 days) partial hepatectomy was performed. The removed two thirds of the liver and the regenerating liver (24 h after partial hepatectomy) were prepared for the biochemical examinations at 4 degrees C. The measurements of the enzyme activities were performed in liver homogenates. The results indicate that the administration of spironolactone cause (1) a weight increase in the normal as well as in the regenerating liver, especially in the older animals; (2) an increase of the protein content of the normal liver in the oldest age-group; (3) a decrease in the DNA content of the normal and regenerating liver of old rats, and (4) an age-dependent increase of decrease of the three lyososomal enzymes beta-glucuronidase, beta-acetylglucosamidase and cathepsin D.
mesh_terms: D000118:Acetylglucosaminidase / Q000378:metabolism; D000375:Aging; D000818:Animals; D002403:Cathepsins / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D005260:Female; D005966:Glucuronidase / Q000378:metabolism; D006498:Hepatectomy; D008099:Liver / Q000033:anatomy & histology / Q000201:enzymology / Q000378:metabolism*; D008115:Liver Regeneration / Q000187:drug effects; D008247:Lysosomes / Q000201:enzymology*; D009929:Organ Size / Q000187:drug effects; D011506:Proteins / Q000378:metabolism; D051381:Rats; D013148:Spironolactone / Q000494:pharmacology*
chemical_list: D011506:Proteins; D013148:Spironolactone; D004247:DNA; D005966:Glucuronidase; D000118:Acetylglucosaminidase; D002403:Cathepsins