Dual role of glucuronyl- and sulfotransferases converting xenobiotics into reactive or biologically inactive and easily excretable compounds.
Glucuronyl- and sulfotransferases inactivate a wide variety of hazardous compounds, for example, phenols and dihydrodiols generated during the metabolism of polycyclic hydrocarbons. Our understanding of the firmly membrane-bound glucuronyltransferase is complicated because of their marked activation by membrane perturbants in vitro. Membrane perturbation also occurs in vivo, for example in liver injury caused by CCl4. Moreover, glucuronyltransferases are inducible by xenobiotics. Phenobarbital and 3-methylcholanthrene probably stimulate separate glucuronyltransferases. Sulfotransferases, located in the cytoplasm, often compete with glucuronyltransferases for the same substrates. The generation of 'active sulfate' (PAPS) from cysteine is more likely to be depleted in vivo than the formation of UDP-glucuronic acid generated from carbohydrates. Hence the proportion of sulfate ester/glucuronide may fall with increasing dose of the substrate. Sulfate esters and glucuronides of certain N-hydroxy-arylamines (N-hydroxy-N-acetylaminofluorene, N-hydroxy-phenacetin) are more reactive than the parent compound and bind covalently to cell constituents. Of the two conjugates, sulfate esters are more reactive and thereby more toxic than the corresponding glucuronides. Glucuronides may become toxic in the kidney and bladder where they are highly concentrated.
D000818:Animals; D001711:Biotransformation; D004789:Enzyme Activation; D004790:Enzyme Induction; D005965:Glucuronates / Q000633:toxicity; D014453:Glucuronosyltransferase / Q000096:biosynthesis / Q000378:metabolism*; D007668:Kidney / Q000187:drug effects; D009281:Naphthalenes / Q000378:metabolism; D010636:Phenols / Q000378:metabolism; D051381:Rats; D013466:Sulfurtransferases / Q000378:metabolism; D001743:Urinary Bladder / Q000187:drug effects
D005965:Glucuronates; D009281:Naphthalenes; D010636:Phenols; D014453:Glucuronosyltransferase; D013466:Sulfurtransferases