[Electrophoretic separation and properties of acid phosphatase isoenzymes from rat brain (author's transl)].
Following agar gel electrophoresis of the 20 000 X g supernatant of rat brain homogenate a single band with acid phosphatase activity could be visualized by means of azo dye techniques. By adding Triton X-100 to the homogenate, a second band with enzymatic activity was demonstrable. A third isoenzyme could be identified in the presence of zinc ions. A fourth band visible only when employing lead methods has to be considered an artefact as its appearance does not depend on the presence of a substrate. The substrate specificity of the isoenzymes and the possibilities of influencing their activity were examined. By specific choice of substrates and effectors, conditions could be defined rendering possible a selective identification of the individual isoenzymes.
D000135:Acid Phosphatase / Q000302:isolation & purification / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000201:enzymology*; D003300:Copper / Q000494:pharmacology; D004587:Electrophoresis, Agar Gel; D005260:Female; D005459:Fluorides / Q000494:pharmacology; D005557:Formaldehyde / Q000494:pharmacology; D005976:Glutaral / Q000494:pharmacology; D006358:Hot Temperature; D007527:Isoenzymes / Q000302:isolation & purification / Q000378:metabolism; D008297:Male; D008982:Molybdenum / Q000494:pharmacology; D011092:Polyethylene Glycols / Q000494:pharmacology; D051381:Rats; D011917:Rats, Inbred Lew; D013379:Substrate Specificity; D013644:Tartrates / Q000494:pharmacology; D015032:Zinc / Q000494:pharmacology
D007527:Isoenzymes; D013644:Tartrates; D005557:Formaldehyde; D011092:Polyethylene Glycols; D003300:Copper; D008982:Molybdenum; D000135:Acid Phosphatase; D015032:Zinc; D005459:Fluorides; D005976:Glutaral
Elektrophoretische Trennung und Eigenschaften von Isoenzymen der sauren Phosphatase aus dem Gehirn der Ratte.