Selective staining of alpha1-antitrypsin (alpha1-protease inhibitor) with Schiff's reagent after separation from serum by analytical isoelectrofocusing in polyacrylamide gel.
After electrofocusing serum from human and various animals in the pH range 4.0-6.0, only the isoproteins of antitrypsin stain with Schiff's reagent. As an additional advantage, this staining provides information about the relative contents of the Schiff-positive carbohydrates of various antitrypsins.
D000178:Acrylamides; D000818:Animals; D002645:Chickens; D006041:Goats; D000882:Haplorhini; D006801:Humans; D007525:Isoelectric Focusing; D008253:Macaca mulatta; D011817:Rabbits; D012394:Rosaniline Dyes; D000515:alpha 1-Antitrypsin / Q000302:isolation & purification*
D000178:Acrylamides; D012394:Rosaniline Dyes; D000515:alpha 1-Antitrypsin