Virulence and immunogenicity of a temperature-sensitive dengue-2 virus in lower primates.
Clones of dengue-2 virus were tested for virulence by inoculation of rhesus monkeys and chimpanzees. Although primates showed no overt signs of illness, inoculation with the parent virus or a subline of a large-plaque clone resulted in a viremia lasting 1 to 7 days. By these criteria, sublines of a small-plaque clone were significantly less virulent and produced little or no viremia in primate hosts. Although they had a substantially reduced viremia, primates inoculated with the small-plaque sublines showed stimulation of complement-fixing, hemagglutination-inhibiting, and neutralizing antibodies. The protection afforded rhesus monkeys 3 months after inoculation with two of the small-plaque sublines was demonstrated by a lack of viremia and a failure to escalate preexisting antibody levels after challenge with the parent virus. Both the S-1 subline and the parent virus had a limited capacity to produce central nervous system pathology in monkeys inoculated intrathalamically and intrathecally. Evidence thus far accumulated for primates indicates that the S-1 subline of dengue-2 virus has potential value as a candidate vaccine virus.
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis*; D001769:Blood / Q000382:microbiology; D003165:Complement System Proteins / Q000378:metabolism; D003715:Dengue / Q000276:immunology / Q000382:microbiology; D003716:Dengue Virus / Q000276:immunology / Q000472:pathogenicity*; D000882:Haplorhini; D006389:Hemagglutinins, Viral; D008251:Macaca / Q000276:immunology*; D008253:Macaca mulatta / Q000276:immunology*; D009154:Mutation; D002679:Pan troglodytes / Q000276:immunology*; D013696:Temperature; D014613:Vaccines, Attenuated; D014765:Viral Vaccines
D000914:Antibodies, Viral; D006389:Hemagglutinins, Viral; D014613:Vaccines, Attenuated; D014765:Viral Vaccines; D003165:Complement System Proteins