Synthesis and secretion of kidney beta-galactosidase in mutant le/le mice.
We have found that the pigmentation mutant light ear in the mouse has a striking effect on lysosomes in the kidney. Male mice homozygous for the le mutant allele have a 4-fold elevated concentration of kidney beta-galactosidase (EC 3.2.1.23). The abnormal elevation of kidney beta-galactosidase is the net result of two processes. First, beta-galactosidase is elevated due to the defective urinary secretion of lysosomal enzymes which is a specific effect of the le mutation. Secondly, this effect is most evident in males and testosterone-treated females because of the induction of beta-galactosidase synthesis by testosterone, which occurs in +/+ as well as in mutant mice. The pale-ear mutation (ep) which mimics the pigmentation phenotype of le also has a similar effect on kidney lysosomes.
D000375:Aging; D000818:Animals; D005260:Female; D005696:Galactosidases / Q000378:metabolism*; D005838:Genotype; D007118:Immunoassay; D007668:Kidney / Q000201:enzymology* / Q000254:growth & development; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009154:Mutation; D012737:Sex Factors; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism*
D005696:Galactosidases; D001616:beta-Galactosidase