[Immunocytochemical labeling of human chromosomes by antibodies from human autoimmune serum].
Mitotic human chromosomes are successively irradiated by ultraviolet, incubated with serum from a patient showing systemic lupus erythematosus (S.L.E.), and antihuman Sheep serum conjugated with peroxidase. After revelation by diaminobenzidine (DAB) banding patterns are pointed out. The chromosome labelling is discussed in comparison with results obtained in other immunocytochemical experiments.
D000906:Antibodies; D001327:Autoimmune Diseases / Q000276:immunology*; D002877:Chromosomes, Human; D006801:Humans; D007124:Immunoenzyme Techniques; D007621:Karyotyping; D008180:Lupus Erythematosus, Systemic / Q000276:immunology; D008214:Lymphocytes / Q000276:immunology / Q000528:radiation effects; D008938:Mitosis; D014466:Ultraviolet Rays
D000906:Antibodies
Marquage immunocytochimique de chromosomes humains après action d'anticorps de sérum autoimmun d'origine humaine.