title: Human fat cell adenylate cyclase: regional differences in adrenaline responsiveness.
abstract: The effect of adrenaline on adenylate cyclase activity in membranes prepared from human abdominal and gluteal adipose tissue was examined. Basal enzyme activity averaged 1.0 mmol cAMP/mg protein/15 min in abdominal tissue and 0.68 nmol cAMP/mg protein/15 min in ghosts from the buttock. Maximally effective concentrations of sodium fluoride (20 mmol/1) induced an eight-fold increase in both membrane preparations. The maximal response to adrenaline (1.0 mmol/1) averaged 170% in ghosts of abdominal tissue compared with 60% in gluteal membranes. The concentrations of adrenaline required to produce half-maximal stimulation were similar in membrane preparations of both regions (about 5 X 10(-5) mol/1).
mesh_terms: D000005:Abdomen; D000262:Adenylyl Cyclases / Q000378:metabolism*; D000273:Adipose Tissue / Q000187:drug effects / Q000201:enzymology*; D000328:Adult; D002081:Buttocks; D004837:Epinephrine / Q000494:pharmacology*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D008297:Male
chemical_list: D000262:Adenylyl Cyclases; D004837:Epinephrine