[Rapid and simultaneous screening and quantitation of antitetanus and anti-HBs antibodies using Laurell's method].
Every transfusion Center which must hunt out anti-tetanus and anti-HBs antibodies to obtain specific corresponding immunoglobulins, is submitted to rapidity, precision and low cost necessity. In order to satisfy these obligations, the authors describe a simple and rapid method, allowing use of the same serum, through a single stage, and simultaneously, screening and quantitation of anti-tetanus antibodies (according to Laurells' method) and of fractionation suitable anti-HBs antibodies (through electro immunodiffusion). Technical conditions exposed in this paper allow direct quantitation of anti-tetanus antibodies from 1 to 30-40 IU; dilutions can be made for upper titers. False negative reactions due to zone effect, as observed in EID and passive haemagglutination do not occur while using this method. Its routine use contributes to the increase of the percentage of fractionation suitable plasmas, without overloading the screening laboratory's tash.
D000907:Antibodies, Bacterial / Q000032:analysis*; D000914:Antibodies, Viral / Q000032:analysis*; D006510:Hepatitis B Antibodies / Q000032:analysis*; D006801:Humans; D005779:Immunodiffusion / Q000379:methods*; D013742:Tetanus / Q000276:immunology*
D000907:Antibodies, Bacterial; D000914:Antibodies, Viral; D006510:Hepatitis B Antibodies
Titrages et dépistages rapides simultanés des anticorps anti-tétaniques et anti-HBs par la technique de Laurell.