Anaerobiosis and oxygen recovery: changes in cell cycle distribution of Ehrlich ascites tumor cells grown in vitro.
The cell cycle distribution of in vitro cultured Ehrlich ascites tumor (EAT) cells was analysed by pulse-cytophotometry to characterize the growth cessation observed under anaerobic conditions. DNA histograms provided evidence that in the absence of oxygen EAT cells accumulate in the G1 and early S phase of the cell cycle while in the presence of oxygen an increase in G2 was observed during 24h culture period. Cellular recovery from anaerobiosis was observed soon after transfer of the cells into fresh aerobic culture medium but occurred slowly if the cells were only resupplied with air. Cell cycle analyses as well as (14C)-thymidine incorporation suggest considerable synchronization results from the introduction of anaerobiosis.
D000693:Anaerobiosis; D000818:Animals; D002250:Carbon Radioisotopes; D002286:Carcinoma, Ehrlich Tumor / Q000378:metabolism / Q000473:pathology*; D002453:Cell Cycle; D002478:Cells, Cultured; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D008660:Metabolism; D051379:Mice; D010100:Oxygen / Q000494:pharmacology*; D010783:Photometry; D013936:Thymidine / Q000378:metabolism
D002250:Carbon Radioisotopes; D004273:DNA, Neoplasm; D010100:Oxygen; D013936:Thymidine