[Studies of the heterogeneity of streptokinases of different origin (author's transl)].
Streptokinases of six different strains of group A streptococci (Strep. pyogenes), and of one strain each of streptococcal groups C and G were isolated from supernatants of the cultures. The purified or partial purified streptokinases were characterized by electrophoresis in agar-acrylamidgels, isoelectric focussing in acrylamide gel and by determinations of the molecular weights by means of sodium dodecylsulphate acrylamide electrophoresis and gel filtration on sephadex G-100. Furtheron these streptokinases were compared serologically using human antistreptokinase-antiserum. It was found that all of the streptokinases examined in this study were different in their isoelectric points (5.05-6.5) and that they showed serological cross-reactions. The molecular weights are not significantly different and were determined to about 45 000 daltons.
D003429:Cross Reactions; D006801:Humans; D007106:Immune Sera; D005779:Immunodiffusion; D007526:Isoelectric Point; D008970:Molecular Weight; D013045:Species Specificity; D013291:Streptococcus / Q000201:enzymology*; D013300:Streptokinase / Q000032:analysis / Q000276:immunology
D007106:Immune Sera; D013300:Streptokinase
Untersuchungen zur Heterogenität von Streptokinasen verschiedener Herkunft.