title: [Ram sperm survival during the use of various solvents after deep freezing in liquid nitrogen].
abstract: Suitability of three diluents for a deep-freezing of ram ejaculates and sperm survival after de-freezing and in the course of a six-hour incubation were investigated. The longest survival was found with the use of diluent containing Tris, fructose, and citric acid: 49.2% after de-freezing and 20.7% after six hours. The influence of the de-freezing procedure on the motility of spermatozoa can also be taken into consideration. The best proved rapid de-freezing without any diluent. The stated results serve for orientation only as the suitability of the diluents will be confirmed by the fertility of inseminated ewes.
mesh_terms: D000818:Animals; D002951:Citrates / Q000494:pharmacology; D005632:Fructose / Q000494:pharmacology; D008297:Male; D009584:Nitrogen / Q000494:pharmacology*; D012662:Semen Preservation; D012756:Sheep / Q000502:physiology*; D012997:Solvents; D013094:Spermatozoa / Q000502:physiology*; D014022:Tissue Survival / Q000187:drug effects*
chemical_list: D002951:Citrates; D012997:Solvents; D005632:Fructose; D009584:Nitrogen
vernacular_title: Prezivatel'nost spermií barana pri pouzití rôznych druhov riedidiel po hlbokom zmrazení v tekutom dusíku.