An efficient method to produce specific anti-actin.
The production of affinity column purified (specific) anti-actin is described. With the immunization scheme employed, all rabbits produced precipitating antibodies over several months, so that 30 mg specific anti-actin per rabbit could be isolated in 6 months. The antibodies against native and detergent denatured smooth muscle actin are characterized by immunodiffusion tests, staining of the I-band of isolated myofibrils and stress fibers in tissue culture cells, using indirect immunofluorescence.
D000199:Actins / Q000276:immunology*; D000818:Animals; D000917:Antibody Formation; D002645:Chickens; D002846:Chromatography, Affinity; D005455:Fluorescent Antibody Technique; D007106:Immune Sera; D005779:Immunodiffusion; D011817:Rabbits / Q000276:immunology
D000199:Actins; D007106:Immune Sera