Stimulation of steroid glucuronidation by uridine diphosphate N-acetylglucosamine and by microsomal disruption.
Glucuronidation of oestrone and of oestradiol in microsomal fractions was markedly and significantly stimulated by UDP-N-acetylglucosamine and by ultrasonication: Triton X-100 also stimulated. This is consistent with compartmentation of UDP-glucuronyl-transferase. Stimulation by UDP-N-acetylglucosamine may be physiologically significant.
D000818:Animals; D004958:Estradiol / Q000378:metabolism*; D004970:Estrone / Q000378:metabolism*; D014453:Glucuronosyltransferase / Q000378:metabolism; D006168:Guinea Pigs; D066298:In Vitro Techniques; D008297:Male; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism*; D014465:Ultrasonics; D014537:Uridine Diphosphate N-Acetylglucosamine / Q000494:pharmacology*; D014539:Uridine Diphosphate Sugars / Q000494:pharmacology*
D014539:Uridine Diphosphate Sugars; D004970:Estrone; D004958:Estradiol; D014537:Uridine Diphosphate N-Acetylglucosamine; D014453:Glucuronosyltransferase