Lipid pigment formation in cultures of chick hepatocytes. Effects of aging and of oxidizable agents.
Tissue cultures of hepatocytes were studied for the formation of lipid pigments as related to aging of the cultures and of the addition to them of: 1) colloidal iron; 2) L-ascorbic acid; and 3) tyrosine. The presence of lipid pigments was gauged by their sudanophilia after a light solvent extraction and yellow autofluorescence. It has been found that all the three agents enhanced pigmentogenesis. It has been concluded that both pro-oxidants and antioxidants may enhance lipid pigment formation: the first by enhancing lipid oxidation, the second by enhancing copolymerization which terminates the oxidative chain.
D000375:Aging; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001205:Ascorbic Acid / Q000494:pharmacology; D002642:Chick Embryo; D007501:Iron / Q000494:pharmacology; D050356:Lipid Metabolism; D008062:Lipofuscin / Q000096:biosynthesis*; D008099:Liver / Q000378:metabolism*; D010084:Oxidation-Reduction; D010860:Pigments, Biological / Q000096:biosynthesis*; D014443:Tyrosine / Q000494:pharmacology
D000975:Antioxidants; D008062:Lipofuscin; D010860:Pigments, Biological; D014443:Tyrosine; D007501:Iron; D001205:Ascorbic Acid