Purification of Pseudomonas aeruginosa exotoxin by affinity chromatography.
Pseudomonas aeruginosa exotoxin A was purified by affinity chromatography from culture supernatants by elution of toxin from antitoxin immunoglobulin G-Sepharose 4B with 3 M NaSCN. The purity, toxicity, and enzymatic activity of exotoxin obtained were comparable to those of toxin purified by previously reported multiple-step procedures.
D000818:Animals; D001427:Bacterial Toxins / Q000032:analysis / Q000302:isolation & purification* / Q000633:toxicity; D002846:Chromatography, Affinity; D003602:Cytotoxicity, Immunologic; D005098:Exotoxins / Q000032:analysis / Q000302:isolation & purification* / Q000633:toxicity; D007074:Immunoglobulin G; D051379:Mice; D011550:Pseudomonas aeruginosa / Q000032:analysis*
D001427:Bacterial Toxins; D005098:Exotoxins; D007074:Immunoglobulin G