Studies on a carboxypeptidase Y mutant of yeast and evidence for a second carboxypeptidase Activity.
Immunological studies on the carboxypeptidase Y mutant prcl-l of Saccharomyces cerevisiae revealed the origin of mutation in the structural gene of carboxypeptidase Y. The absence of carboxypeptidase Y has no effect on growth, even after drastic changes of growth conditions. A double mutant (prc 1- leu2-) lacking carboxypeptidase Y and auxotrophic for leucine is able to grow on the peptide benzyloxycarbonylglycylleucine (Cbz-Gly-Leu) as sole nitrogen source, indicating the existence of a second carboxypeptidase. Using a new peptidase test, the existence of this second enzyme, called carboxypeptidase S, was confirmed biochemically.
D002268:Carboxypeptidases / Q000276:immunology / Q000378:metabolism*; D004492:Edetic Acid / Q000494:pharmacology; D006238:Haploidy; D007118:Immunoassay; D005779:Immunodiffusion; D007700:Kinetics; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D013045:Species Specificity; D013329:Structure-Activity Relationship
D004492:Edetic Acid; D002268:Carboxypeptidases