[In vitro production of hemopoietic stem cells: washings from organ cultures of embryonic liver].
Hemopoietic cells including CFUs could be washed off from the organ culture of fetal liver periodically for 4 weeks. Under the cultivation conditions employed this treatment did not reduce the CFUs content of the culture essentially; thus, the washings off could be used to elevate the CFUs yield per culture.
D000818:Animals; D002452:Cell Count; D003114:Colony-Forming Units Assay; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D008099:Liver / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009924:Organ Culture Techniques / Q000379:methods; D013997:Time Factors
Produktsiia stvolovykh krovetvornykh kletok in vitro--smyvy s organnykh kultur émbrional'noĭ pecheni.