Subtyping of hepatitis B surface antigen and antibody by radioimmunoassay.
The hepatitis B surface antigen (HBSAg) has been shown to possess distinct subtypes adw, ayw, adr, and ayr). A commercially available solid phase radioimmunoassay for antibody to HBSAg (Ausab, Abbott Laboratories North Chicago, Ill.) has been modified to detect the subtypes of HBSAg as well as the subtype-specific anti-HBS reactivities to detect the subtypes of HBSAg as well as the subtype-specific anti-HBS reactivities (anti-d, anti-y, and anti-w). This method has the advantages of general availability, ease of performance, and increased sensitivity over conventional subtyping methods of agar gel diffusion and counterelectrophoresis.
D000362:Agar; D000818:Animals; D000914:Antibodies, Viral / Q000145:classification*; D001782:Blood Donors; D003378:Counterimmunoelectrophoresis; D006509:Hepatitis B / Q000276:immunology; D006514:Hepatitis B Surface Antigens / Q000145:classification*; D006520:Hepatitis, Animal / Q000276:immunology; D006801:Humans; D005779:Immunodiffusion; D007202:Indicators and Reagents; D009316:National Institutes of Health (U.S.); D002679:Pan troglodytes; D011863:Radioimmunoassay; D014481:United States
D000914:Antibodies, Viral; D006514:Hepatitis B Surface Antigens; D007202:Indicators and Reagents; D000362:Agar