title: Characterization of dog small intestinal fucolipids with human blood group A activity. Differences in dog and human A-active fucolipids.
abstract: Glycolipids containing fucose (fucolipids) which carried human blood group A activity were isolated from a number of dog small intestines and analyzed. On the basis of sugar analysis, methylation, periodate oxidation, enzyme degradation, mass spectrometry, and immunologic studies, a structure is proposed for these substances. The ceramides of the dog fucolipids containing only hydroxylated fatty acids with 85% saturated and 15% monoenoic acids ranging from 16 to 25 carbon atoms. Sphingosine and phytosphingosine comprised 48% each of the long chain bases. An A-active fraction isolated from human small intestine was shown to have two components, one of which was immunologically distinct and the other identical with the dog intestinal fucolipids. The human fraction differed from the dog fucolipids in migration on thin-layer chromatography and contained two types of amino sugar substitution. It is proposed that the human fraction was composed of two fucolipids with incomplete structures.
mesh_terms: D000017:ABO Blood-Group System; D000818:Animals; D002241:Carbohydrates / Q000032:analysis; D002849:Chromatography, Gas; D004285:Dogs; D005227:Fatty Acids / Q000032:analysis; D005643:Fucose / Q000032:analysis; D006017:Glycolipids / Q000302:isolation & purification; D006801:Humans; D005779:Immunodiffusion; D007421:Intestine, Small / Q000032:analysis*; D013058:Mass Spectrometry; D013045:Species Specificity
chemical_list: D000017:ABO Blood-Group System; D002241:Carbohydrates; D005227:Fatty Acids; D006017:Glycolipids; D005643:Fucose