Delayed-type hypersensitivity against Semliki Forest virus in mice.
After intracutaneous immunization with purified inactivated Semliki Forest virus, a delayed-type hypersensitivity without detectable antibodies in serum was obtained in BALB/c mice. Low doses of antigen given intraperitoneally induced antibodies. Intracutaneous immunization with much higher doses induced no specific antibodies, but a footpad swelling was observed after challenge with homolgous antigen. Pretreatment with cyclophosphamide before immunization enhanced footpad swelling. Microscopic examination of footpads from sensitized mice at 24 h after challenge showed a mononuclear infiltrate. The delayed-type reaction could be transferred to syngenic mice with lymph node cells, but not with spleen cells or serum. The biphasic character of the delayed-type hypersensitivity is discussed.
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis; D000956:Antigens, Viral; D003520:Cyclophosphamide / Q000494:pharmacology; D006968:Hypersensitivity, Delayed; D007114:Immunization; D007116:Immunization, Passive; D008198:Lymph Nodes / Q000166:cytology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D012672:Semliki forest virus / Q000276:immunology*; D013154:Spleen / Q000166:cytology
D000914:Antibodies, Viral; D000956:Antigens, Viral; D003520:Cyclophosphamide