The N-terminal activation fragment of bovine prothrombin. Immunological studies leading to a one step purification.
Some immunological studies on prothrombin fragment 1 from bovine prothrombin and its warfarin-induced precursor acarboxyprothrombin are reported. Based on the results, a rapid and simple immunoadsorption method for the isolation of prothrombin fragment 1 in good yield has been established. The method exploits the conformational change induced in the fragment by removal of Ca2+. The principle may be applicable to other gamma-carboxyglutamyl-containing proteins or fragments therof.
D001059:Apoproteins; D002118:Calcium; D004492:Edetic Acid; D004800:Enzymes, Immobilized / Q000378:metabolism; D005779:Immunodiffusion; D007700:Kinetics; D010446:Peptide Fragments / Q000302:isolation & purification; D011516:Prothrombin / Q000302:isolation & purification / Q000378:metabolism; D014859:Warfarin
D001059:Apoproteins; D004800:Enzymes, Immobilized; D010446:Peptide Fragments; D014859:Warfarin; D011516:Prothrombin; D004492:Edetic Acid; D002118:Calcium