Extraction and purification of specific antibody against Pseudomonas aeruginosa by immune affinity chromatography.
Immune affinity chromatography has been used to extract and purify specific immune antibody against P. aeruginosa immunotype 4 from hyperimmune gamma glubulin containing antibody against each of seven major immunotypes of P. aeruginosa. A column with a 4 ml. bed volume was construced which had a "working capacity" of approximately 1,680 agglutination units. With water used as the eluting agent, 100 percent yields of functionally active antibody were obtained when amounts equal to or less than the working capacity were loaded onto the column. The technique appears to be suitable for large scale extraction of specific immune antibody for therapeutic trials or investigative use.
D000818:Animals; D000907:Antibodies, Bacterial / Q000302:isolation & purification*; D001666:Binding Sites, Antibody; D002846:Chromatography, Affinity; D011135:Polysaccharides, Bacterial / Q000276:immunology; D011550:Pseudomonas aeruginosa / Q000276:immunology*
D000907:Antibodies, Bacterial; D011135:Polysaccharides, Bacterial