Nitration of polypeptides using ethanol in reaction buffers minimizes crosslinking.
High concentrations of ethanol are effective in reducing the intermolecular cross-linking which predominates when the reactions of tetranitromethane with the carboxypeptidase inhibitor from potatoes, a chymotrypsin inhibitor also from potatoes, and insulin are carried out under standard conditions. Including reagents such as ethanol may be of general utility in the preparation of monomeric nitro-derivatives of hydrophobic, low-molecular-weight proteins.
D002021:Buffers; D055598:Chemical Phenomena; D002621:Chemistry; D002852:Chromatography, Ion Exchange; D002918:Chymotrypsin / Q000037:antagonists & inhibitors; D003432:Cross-Linking Reagents; D000431:Ethanol; D007202:Indicators and Reagents; D007328:Insulin; D008697:Methane / Q000031:analogs & derivatives; D010940:Plant Proteins; D011480:Protease Inhibitors; D013774:Tetranitromethane
D002021:Buffers; D003432:Cross-Linking Reagents; D007202:Indicators and Reagents; D007328:Insulin; D010940:Plant Proteins; D011480:Protease Inhibitors; D000431:Ethanol; D002918:Chymotrypsin; D013774:Tetranitromethane; D008697:Methane