Acetylation of the NH2-terminal serine of prostaglandin synthetase by aspirin.
Aspirin (acetylsalicylic acid) inhibits prostaglandin synthesis by acetylating an active site portion of the enzyme, prostaglandin synthetase. In the current study, the site of acetylation has been demonstrated to be a seryl residue at the NH2 terminus of the enzyme. Purified [3H]acetyl enzyme was prepared from seminal vesicle homogenates treated with [acetyl-3H]aspirin. The [3H]acetate to protein bond was stable to hydroxylamine, indicating an N-acetyl linkage. The [3H]acetyl enzyme was fragmented sequentially with cyanogen bromide, trypsin, and pronase. The 3H material isolated from the pronase digest was identified as N-acetylserine. This finding indicates that the oxygenase portion of prostaglandin synthetase has an NH2-terminal serine which is involved in enzymatic activity and is susceptible to acetylation by aspirin.
D000107:Acetylation; D000818:Animals; D001241:Aspirin; D002417:Cattle; D016861:Cyclooxygenase Inhibitors; D008297:Male; D010446:Peptide Fragments / Q000032:analysis; D012669:Seminal Vesicles / Q000201:enzymology; D012694:Serine; D012756:Sheep
D016861:Cyclooxygenase Inhibitors; D010446:Peptide Fragments; D012694:Serine; D001241:Aspirin