Effects of temperature and bovine serum albumin on lysis of erythrocytes induced by dilauroylglycerophosphocholine and didecanoylglycerophosphocholine.
The effects of the incubation temperature and bovine serum albumin on hemolysis induced by short-chain phosphatidylcholine were examined. The rate of hemolysis of human, monkey, rabbit, and rat erythrocytes by dilauroylglycerophosphocholine showed biphasic temperature-dependence: hemolysis was rapid at 5-10 degrees C and above 40 degrees C, but slow at around 25 degrees C. In contrast, the rate of lysis of cow, calf, sheep, pig, cat, and dog erythrocytes did not show biphasic temperature-dependence, but increased progressively with increase in the incubation temperature. Bovine serum albumin increased the hemolysis of human erythrocytes induced by dilauroylglycerophosphocholine or didecanoylglycerophosphocholine: it shortened the lag time of lysis and reduced the amount of phosphatidylcholine required for lysis. A shift-down of the incubation temperature from 40 to below 10 degrees C also shortened the lag time of lysis of human erythrocytes induced by dilauroylglycerophosphocholine and reduced the amount of phosphatidylcholine required for lysis.
D000818:Animals; D002415:Cats; D002417:Cattle; D004285:Dogs; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000187:drug effects*; D005260:Female; D000882:Haplorhini; D006461:Hemolysis / Q000187:drug effects; D009996:Osmotic Fragility / Q000187:drug effects; D010713:Phosphatidylcholines / Q000494:pharmacology*; D011817:Rabbits; D051381:Rats; D012710:Serum Albumin, Bovine / Q000494:pharmacology*; D012756:Sheep; D013552:Swine; D013696:Temperature
D010713:Phosphatidylcholines; D012710:Serum Albumin, Bovine