Multiacetylated forms of H4 are found in a putative transcriptionally competent chromatin fraction from trout testis.
We have examined the distribution of acetylated histones derived from various trout testis chromatin fractions of different composition. Our results indicate that a chromatin fraction, preferentially solubilized by micrococcal nuclease, containing the bulk of the HMG proteins and similar to a fraction released from intact trout nuclei and previously shown to be enriched in transcribed DNA sequences also possesses high levels of multiacetylated species of H4. Histones 2A, 2B and 3 are also acetylated in this particular chromatin fraction. Monoacetylated species of the 4 inner nucleosomal histones appear to be characteristic of the nucleohistone portion of trout testis chromatin.
D000085:Acetates / Q000378:metabolism; D000107:Acetylation; D000818:Animals; D002843:Chromatin / Q000378:metabolism*; D006657:Histones / Q000302:isolation & purification / Q000378:metabolism; D008297:Male; D008970:Molecular Weight; D013737:Testis / Q000378:metabolism*; D014158:Transcription, Genetic; D014337:Trout
D000085:Acetates; D002843:Chromatin; D006657:Histones