[A definitive identification method: gas-liquid chromatography].
The definitive identification of many anaerobes requires the use of gas-liquid chromatography to detect alcohol and volatile acids end-products of their metabolism. In this study we have used the gas-liquid chromatography for the identification of 360 non sporing anaerobes isolated from faeces of healthy individuals. These analyses were performed on a Variant-Aerograph mod. 2700 gas chromatography fitted with a column without phosphoric acid. The results showed a good percentage of typing (83%).
D000191:Actinomycetaceae / Q000145:classification*; D000438:Alcohols / Q000378:metabolism; D000693:Anaerobiosis; D001419:Bacteria / Q000378:metabolism; D001431:Bacteriological Techniques; D001438:Bacteroidaceae / Q000145:classification*; D002849:Chromatography, Gas; D005232:Fatty Acids, Volatile / Q000378:metabolism; D007778:Lactobacillus / Q000145:classification*; D011423:Propionibacteriaceae / Q000145:classification*
D000438:Alcohols; D005232:Fatty Acids, Volatile
Metodi di identificazione definitiva: cromatografia gas-liquido.