[Antigen detection in substrates containing immune antigen-antibody complexes].
Treatment of immune complexes consisting of different antigens and corresponding IgG with low doses of hydrochloric cystein led to the antibody inactivation, to the complex splitting, and to the release of the antigen. Antibodies being a part of the complex retained their capacity to react with the antiglobulin serum. The optimal doses of cystein leading to the complex splitting and to the IgG inactivation in the immune complex composition failed to act on unbound IgG. The effect of other reducing agents (glutathion and sodium sulfite) on the immune complex was similar to that of cystein. The differences in the effect of cystein on unbound and antigen-bound antibodies indicated that apparantly the combining site of antibody served as a point of cystein application.
D000818:Animals; D000936:Antigen-Antibody Complex; D000941:Antigens / Q000032:analysis*; D002417:Cattle; D003168:Complement Fixation Tests; D003545:Cysteine / Q000276:immunology; D004305:Dose-Response Relationship, Drug; D006801:Humans; D005779:Immunodiffusion; D007074:Immunoglobulin G; D011135:Polysaccharides, Bacterial / Q000276:immunology; D013297:Streptococcus pyogenes / Q000276:immunology
D000936:Antigen-Antibody Complex; D000941:Antigens; D007074:Immunoglobulin G; D011135:Polysaccharides, Bacterial; D003545:Cysteine
Vyiavlenie antigena v substratakh, soderzhashchikh immunnye kompleksy antigen-antitelo.