Culture of mammalian prostatic cells.
Preliminary experimental data and concepts oriented toward the achievement of certified primary prostatic cell cultures, normal and/or malignant, for mass distribution are presented. Such monolayer cultures would be established as subcultures from the microcarrier, spin-filter (steady-state) system. The suspension culture of primary cells directly derived from the tissue of origin would be propagated at high cell populations for extended periods of time. The ease of complete monitoring on a sequential basis for conformity to specifications is apparent. The feasibility of the approach appears to have been established. Problems relating to the reduction to practice are detailed.
D000135:Acid Phosphatase / Q000096:biosynthesis; D000818:Animals; D002478:Cells, Cultured; D003470:Culture Media; D000882:Haplorhini; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D010215:Papio; D011467:Prostate / Q000166:cytology* / Q000201:enzymology
D003470:Culture Media; D000135:Acid Phosphatase