[Methods of evaluating the function of transplanted islets of Langerhans (author's transl)].
The various groups who are working on islet-transplantation used different parameters in determining the success or failure of their experiments. In our own transplantation-trials we tested the value of using the symptoms typical of diabetic humans for rats. These are: change in bodyweight, water intake, urine-volume and glucose content as well as the glucose-level in the blood. Special diabetic provocation-tests were also carried out with rats. It was shown, that the i.v. glucose-tolerance-test (IVGTT) and the glucose assimilation-coefficient (GAC) were able to demonstrate the reversal of the diabetic state after islet-transplantation. The tolbutamid-test gave no additional evidence about the success of the transplantation. Histological examinations should always be carried out in addition to the biochemical tests, because a decrease in the islet's-function is shown to depend on a higher rate of morphological regressions.
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight; D003920:Diabetes Mellitus / Q000503:physiopathology / Q000628:therapy*; D004195:Disease Models, Animal; D004327:Drinking Behavior; D005951:Glucose Tolerance Test / Q000379:methods; D006029:Glycosuria; D007328:Insulin / Q000097:blood; D016381:Islets of Langerhans Transplantation; D010179:Pancreas / Q000601:surgery*; D051381:Rats; D012107:Research Design; D014044:Tolbutamide; D014184:Transplantation, Homologous; D014556:Urine / Q000378:metabolism
D001786:Blood Glucose; D007328:Insulin; D014044:Tolbutamide
Methoden zur Funktionskontrolle transplantierter Langerhansscher Inseln