Sensitive enzyme-linked immunosorbent assay for detection of antibodies against typhus rickettsiae, Rickettsia prowazekii and Rickettsia typhi.
An enzyme-linked immunosorbent assay (ELISA) has been developed for the titration of rickettsial antibodies in human and animal sera. Two preparations of soluble typhus-group antigens were obtained from Rickettsia typhi and Rickettsia prowazekii by ether extraction: a standard antigen from infected yolk sacs (YS antigen) and one free of yolk sac contaminants from Renografin-purified rickettsiae (PR antigen). Rabbit, mouse, and guinea pig sera were obtained by immunization with viable purified R. typhi or R. prowazekii. Human sera were obtained from individuals who had recovered from laboratory infections with either typhus rickettsia months or years previously. Goat-derived anti-immunoglobulins were conjugated to alkaline phosphatase with glutaraldehyde. Although the PR and YS antigens gave equivalent antibody titers in the complement fixation test, the PR antigen was clearly superior in the ELISA. With this antigen, the titration curves of all antisera were linear over a wider range of serum concentrations and the titers were higher than with the YS antigen. With YS and PR antigens, ELISA titers were higher than those obtained by complement fixation by one and two orders of magnitude, respectively. In human sera, immunoglobulin G and immunoglobulin M antibodies were demonstrated by their respective anti-immunoglobulins and by differential susceptibility to ethanethiol. ELISA titers showed some type specificity, whereas none was observed in complement fixation tests. The ELISA is highly sensitive, reproducible, and easily adaptable to the various requirements of clinical and research laboratories.
D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis*; D000942:Antigens, Bacterial; D003168:Complement Fixation Tests; D004797:Enzyme-Linked Immunosorbent Assay; D004980:Ethane / Q000031:analogs & derivatives / Q000494:pharmacology; D006168:Guinea Pigs; D006801:Humans; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D051379:Mice; D011817:Rabbits; D012283:Rickettsia prowazekii / Q000276:immunology*; D012286:Rickettsia typhi / Q000276:immunology*; D013438:Sulfhydryl Compounds / Q000494:pharmacology
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007106:Immune Sera; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D013438:Sulfhydryl Compounds; D004980:Ethane; C007638:ethanethiol