[Viral RNAs from cells infected by an oncornavirus. I. Characterization of viral RNAs associated with cytoplasmic particles].
Oncornaviral particles on the way of their synthesis have been isolated from cytoplasm of a Friend virus-producing murine cell line. By use of electron microscopy and retrotranscriptase assay, it was found that the majority of the viral structures is pelleted by sedimentation at 90.000 g. The Poly (A) RNA of this pellet contains principally 70S and 35S RNA species which, as judged by size determination and hybridization with Friend-virus c-DNA are the species embedded into the virions. We verified that conditions which were used in the course of isolation, effectively dissociate polysomes and prevent them from co-sedimenting with viruses.
D002460:Cell Line; D003593:Cytoplasm / Q000648:ultrastructure; D004492:Edetic Acid; D005622:Friend murine leukemia virus / Q000032:analysis* / Q000648:ultrastructure; D008274:Magnesium; D008854:Microscopy, Electron; D008970:Molecular Weight; D009693:Nucleic Acid Hybridization; D011061:Poly A / Q000032:analysis; D012367:RNA, Viral / Q000302:isolation & purification
D012367:RNA, Viral; D011061:Poly A; D004492:Edetic Acid; D008274:Magnesium
RNAs viraux des cellules infectées par un oncornavirus: I. - Caractérisation des RNAs viraux associés à des particules cytoplasmiques.