Labeling of platelets with oxine complexes of Tc-99m and In-111. Part 1. In vitro studies and survival in the rabbit.
We have used both Tc-99m oxine and In-111 oxine to study the effects in the rabbit of various parameters on platelet labeling and the in vivo survival of platelets harvested by four different methods. For In-111 oxine, platelet labeling in saline produces much higher efficiencies (90%) than labeling in the presence of plasma (20%), with no significant shortening of in vivo survival (1% survival at 5.9 days). For Tc-99m oxine, labeling efficiencies are considerably lower (30%) and survival is shorter (1% survival at 3.8 days). For both radioagents, a 30-min labeling incubation at room temperature is suggested.
D000818:Animals; D001792:Blood Platelets; D002470:Cell Survival; D006912:Hydroxyquinolines; D066298:In Vitro Techniques; D007204:Indium; D007553:Isotope Labeling / Q000379:methods*; D015125:Oxyquinoline; D011817:Rabbits; D011868:Radioisotopes; D013667:Technetium
D006912:Hydroxyquinolines; D011868:Radioisotopes; D007204:Indium; D015125:Oxyquinoline; D013667:Technetium