An evaluation of a simple polyacrylamide gel electrophoresis system for lipoproteins and its use for the characterisation of hyperlipoproteinaemia.
The use of polyacrylamide gel electrophoresis as a screening procedure for the characterisation of hyperlipoproteinaemia is described. Sixty-one samples were investigated and classified by four different methods: (1) chemical profile derived from visual appearance and estimation of total cholesterol and triglyceride; (2) 'SML' profile by nephelometry; (3) electrophoresis on agarose gel; and (4) electrophoresis on polyacrylamide gel. Polyacrylamide gel electrophoresis showed the closest correlation with the chemical profile when compared with the other two methods. Polyacrylamide gel electrophoresis was found to be a rapid, reliable, and satisfactory procedure for plasma lipoprotein phenotyping.
D001797:Blood Protein Electrophoresis; D001798:Blood Proteins / Q000032:analysis*; D002784:Cholesterol / Q000097:blood; D004587:Electrophoresis, Agar Gel; D004591:Electrophoresis, Polyacrylamide Gel; D005069:Evaluation Studies as Topic; D006801:Humans; D006949:Hyperlipidemias / Q000097:blood*; D008074:Lipoproteins / Q000097:blood*; D009391:Nephelometry and Turbidimetry; D014280:Triglycerides / Q000097:blood
D001798:Blood Proteins; D008074:Lipoproteins; D014280:Triglycerides; D002784:Cholesterol