A new procedure for purifying histone pairs H2A + H2B and H3 + H4 from chromatin using hydroxylapatite.
A method to purify histone groups H2A+H2B and H3+H4 using dissociation with NaCl and hydroxylapatite chromatography is presented. The procedure is simple, involves mild solvents, and provides milligram quantities of histones of high purity. The histone pairs prepared by this method can regenerate chromatin-like characteristics when combined and reconstituted with DNA.
D000818:Animals; D002645:Chickens; D002843:Chromatin / Q000032:analysis*; D002845:Chromatography / Q000379:methods; D004372:Ducks; D004912:Erythrocytes / Q000032:analysis; D006657:Histones / Q000097:blood / Q000302:isolation & purification*; D006882:Hydroxyapatites
D002843:Chromatin; D006657:Histones; D006882:Hydroxyapatites