Evidence for participation of cytochrome b5 in microsomal delta-6 desaturation of fatty acids.
The delta-6 desaturation of linoleic acid to gamma-linolenic acid and oleic acid to 6,9-octadecadienoic acid by rat liver microsomes was investigated. Using a specific antibody prepared against purified rat liver cytochrome b5, we demonstrated that cytochrome b5 participated in delta-6 desaturation of both fatty acids. The reaction products were identified as their methyl ester derivatives by argentation thin-layer chromatography, gas-liquid chromatography, and reductive ozonolysis followed by gas-liquid chromatography.
D000818:Animals; D003580:Cytochromes / Q000276:immunology / Q000378:metabolism*; D044943:Fatty Acid Desaturases / Q000378:metabolism; D007074:Immunoglobulin G / Q000494:pharmacology; D008041:Linoleic Acids / Q000378:metabolism*; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D009829:Oleic Acids / Q000378:metabolism*; D051381:Rats
D003580:Cytochromes; D007074:Immunoglobulin G; D008041:Linoleic Acids; D009829:Oleic Acids; D044943:Fatty Acid Desaturases; D009251:NADPH-Ferrihemoprotein Reductase