[Immunofluorescence study of the formation of evolutionary stable lens proteins in chick embryos].
In the lens of fishes (carp, spiny dogfish) beta-crystallins were identified which were characteristic also of reptiles, amphibians, birds and mammals (evolutionary stable beta-crystallins). The dynamics of the formation of such beta-crystallins in 5--14 days old chick embryos was studied by the indirect immunofluorescence method with antisera to fish lens. These proteins are reliably indentified first at the lens sections from 7--8days old chick embryos. At all stages under study these beta-crystallins are localized mainly in the epithelial cells and practically not found in the lens fibers. They were, however, found in the fibrous (central) part of developing lens as well by the method of immunoelectrophoresis.
D000367:Age Factors; D000818:Animals; D005075:Biological Evolution; D002347:Carps; D002642:Chick Embryo; D003459:Crystallins / Q000032:analysis* / Q000096:biosynthesis; D005455:Fluorescent Antibody Technique; D005779:Immunodiffusion; D007122:Immunoelectrophoresis; D007908:Lens, Crystalline / Q000032:analysis; D012754:Sharks; D013045:Species Specificity
D003459:Crystallins
Immunofluorestsentnoe izuchenie formirovaniia évoliutsionno-ustoichivykh belkov khrustalika u zarodysheĭ kur.