Interaction of ethidium bromide with ribosomes. Absorption, fluorescence, circular dichroism and sedimentation studies.
It is shown in this work that the binding of ethidium bromide to yeast ribosomes occurs through intercalation in the double-stranded rRNA regions and produces changes in the ribosomes structure, yielding unfolded subparticles, and even partial separation of proteins from rRNA at very high binding ratios. The addition of Mg2+ prevents these structural changes, probably by partial inhibition of the dye binding.
D001665:Binding Sites; D002942:Circular Dichroism; D004492:Edetic Acid / Q000494:pharmacology; D004996:Ethidium / Q000378:metabolism*; D008274:Magnesium / Q000494:pharmacology; D012335:RNA, Ribosomal / Q000378:metabolism; D012270:Ribosomes / Q000187:drug effects / Q000378:metabolism*; D012441:Saccharomyces cerevisiae; D013050:Spectrometry, Fluorescence; D014461:Ultracentrifugation
D012335:RNA, Ribosomal; D004492:Edetic Acid; D004996:Ethidium; D008274:Magnesium