Characterization of amyloid of ageing obese-hyperglycaemic mice and their lean littermates.
Amyloid fibrils, isolated from 18-month-old obese-hyperglycaemic mice and their lean littermates, were characterized immunologically and chemically. The main amyloid fibril subunit protein was protein AA, which cross-reacted completely with an antiserum against amyloid from mice with experimentally induced amyloidosis and had an amino acid composition and N-terminal amino acid sequence identical to that protein. These results indicate that the spontaneously occurring amyloidosis in obese-hyperglycaemic mice and their lean littermates corresponds to human, secondary amyloidosis and may serve as a model for that disease.
D000375:Aging; D000595:Amino Acid Sequence; D000596:Amino Acids / Q000032:analysis; D000682:Amyloid / Q000032:analysis* / Q000276:immunology; D000686:Amyloidosis / Q000276:immunology / Q000378:metabolism; D000818:Animals; D002850:Chromatography, Gel; D003429:Cross Reactions; D004586:Electrophoresis; D006943:Hyperglycemia / Q000276:immunology / Q000378:metabolism*; D007106:Immune Sera; D008297:Male; D051379:Mice; D008820:Mice, Obese; D000685:Serum Amyloid A Protein / Q000032:analysis
D000596:Amino Acids; D000682:Amyloid; D007106:Immune Sera; D000685:Serum Amyloid A Protein