[Determination of the kinetic parameters of enzyme inhibition of the K-type mechanism: application to the control of alpha-glucosidase activity of honeybee hemolymph by glucose].
The alpha-glucosidasic activity of emerging honeybees haemolymph is submitted to a feed-back inhibition by glucose, according to a mechanism of the "K" type (competitive). The "resulting affinity-constant" (measured in the presence of the enzyme both with substrate and inhibitor) is linear function of the inhibitor concentration. The affinity constants between enzyme and pure substrate on one hand, and between enzyme and pure inhibitor on the other hand, were determined by means of this relation, which led to respectively equivalent values after determinations under in vitro or in vivo inhibitions.
D000818:Animals; D001516:Bees; D001667:Binding, Competitive; D005246:Feedback; D005947:Glucose / Q000494:pharmacology*; D005959:Glucosidases / Q000037:antagonists & inhibitors*; D065089:Glycoside Hydrolase Inhibitors; D006458:Hemolymph / Q000201:enzymology*; D007700:Kinetics; D008433:Mathematics
D065089:Glycoside Hydrolase Inhibitors; D005959:Glucosidases; D005947:Glucose
Détermination des paramètres d'inhibition enzymatique dans le cas d'un mécanisme de type K : application au contrle de l'activé alpha-glucosidasique (1) de l'hémolymphe d'abeilles par le glucose.