Combined histochemical and x-ray microanalytical studies on the copper-accumulating granules in the mid-gut of larval Drosophila.
The copper-containing granules in the mid-gut epithelium of larval Drosophila melanogaster were examined for acid phosphatase by combined histochemistry and energy-dispersive, X-ray microanalysis. After incubation, many of the granules were shown to contain simultaneously copper and sulphur (which are normal constituents), and lead and phosphorus (which are the detectable elements of the reaction product). Earlier work has been consolidated and extended and the evidence that the granules are formed as cytolysosomes is reviewed.
D000135:Acid Phosphatase / Q000378:metabolism; D003300:Copper / Q000378:metabolism*; D003594:Cytoplasmic Granules / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000378:metabolism*; D004577:Electron Probe Microanalysis; D006651:Histocytochemistry; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000201:enzymology / Q000648:ultrastructure; D007814:Larva / Q000378:metabolism; D013455:Sulfur / Q000032:analysis
D013455:Sulfur; D003300:Copper; D000135:Acid Phosphatase