Chromatographic fractionation of Escherichia coli transfer RNA on a new support, naphthoyl-Sepharose.
The noncharged naphthoyl-Sepharose CL-6B has been prepared. Escherichia coli tRNA binds to this new adsorbent in 0.75 M ammonium sulphate at neutral pH at room temperature. Using a negative salt gradient, the tRNAs are eluted in a defined order. The chromatographic pattern is clearly different from those of other commonly used tRNA separation techniques.
D002845:Chromatography / Q000379:methods*; D004926:Escherichia coli / Q000032:analysis; D011134:Polysaccharides; D012343:RNA, Transfer / Q000302:isolation & purification*; D012685:Sepharose / Q000031:analogs & derivatives
D011134:Polysaccharides; C027105:naphthoyl-sepharose; D012685:Sepharose; D012343:RNA, Transfer