[Nonspecific esterase and naphthol-AS-D-chloroacetate esterase in monocytoid and myeloid cells of healthy cattle and cattle suffering from leukosis].
The reaction to non-specific esterase can be used for the identification of the monocytoid cells of the periphery. A negative reaction is exhibited by neutrophile and eosinophile leucocytes and erythrocytes. Varying results are obtained from lymphocytes, rendering it impossible to use this method in the group of lymphoid cells of the periphery or marrow. In the group of large marrow cells (promonocytes), non-specific esterase gave a very strong reaction; this applies both to the marrow of healthy cattle and cattle suffering from leucosis. For the time being, efforts to use this reaction for the solution of the problem of the differentiation of monocytoid cells and cells of similar size in the myeloid series of the bone marrow have not been successful. In neutrophile leucocytes of the periphery, naphtol-AS-D-chloroacetate esterase gives a less intensive reaction than in humans. For this reason, it is less suitable for the differentiation of these cells. Other cell types (eosinophile leucocytes, monocytes, lymphocytes, erythrocytes as well as their bone-marrow stages) give a negative reaction. In the group of large marrow cells of the myeloid series (promyelocytes and neutrophile myelocytes), naphtol-AS-D-chloracetate esterase shows a more intensive reaction in healthy cattle, as compared with cattle suffering from leucosis.
D000818:Animals; D002265:Carboxylic Ester Hydrolases / Q000097:blood*; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis*; D004796:Clinical Enzyme Tests; D004804:Eosinophils / Q000201:enzymology; D004912:Erythrocytes / Q000201:enzymology; D004950:Esterases / Q000097:blood; D007938:Leukemia / Q000662:veterinary*; D008214:Lymphocytes / Q000201:enzymology; D009000:Monocytes / Q000201:enzymology; D009283:Naphthol AS D Esterase / Q000097:blood*; D009504:Neutrophils / Q000201:enzymology
D004950:Esterases; D009283:Naphthol AS D Esterase; D002265:Carboxylic Ester Hydrolases
Nespecifická esteráza a naftol-AS-D-chloracetátesteráza u monocytoidních a myeloidních bunék zdravého a leukózního skotu.