[Role of phospholipase A2 in anoxic injury to the energy-dependent functions of the mitochondria].
An increase in concentration of free fatty acids was observed simultaneously with a decrease in energy dependent functions of mitochondria (oxidative phosphorylation and Ca2+-accumulating ability) after their preincubation under anoxic conditions in vitro with Ca2+ ions (11-14 nmol/mg of mitochondrial protein). Impairment of mitochondria and accumulation of fatty acids were prevented by inhibitors of phospholipase A2 from mitochondria. The anoxic impairment of mitochondria appears to be caused by Ca2+-activated phospholipase A2.
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002413:Cations, Divalent; D002712:Chlorides; D003300:Copper / Q000494:pharmacology; D003992:Dibucaine / Q000494:pharmacology; D004533:Egtazic Acid / Q000494:pharmacology; D004734:Energy Metabolism / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D000860:Hypoxia / Q000201:enzymology*; D066298:In Vitro Techniques; D008297:Male; D008565:Membrane Proteins; D008930:Mitochondria, Liver / Q000187:drug effects / Q000201:enzymology*; D010101:Oxygen Consumption / Q000187:drug effects; D010740:Phospholipases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051381:Rats
D002413:Cations, Divalent; D002712:Chlorides; D005230:Fatty Acids, Nonesterified; D008565:Membrane Proteins; D004533:Egtazic Acid; D003300:Copper; D010740:Phospholipases; D003992:Dibucaine; D002118:Calcium
Rol' fosfolipazy A2 v anoksicheskom povrezhdenii énergozavisimykh funktsiĭ mitokhondriĭ.