Structural restriction and similarities of the light chains of antibodies to group B streptococcal carbohydrate produced in a single rabbit.
Comparison of the amino acid compositions, N-terminal amino acids and tryptic peptide maps of two L chains (145 LI and 145 LII) derived from antibodies produced in a single rabbit to group B streptococci indicates a high degree of sequence homology of the two chains. Furthermore, comparison of these data with similar analysis of L chains prepared from pooled antibodies to group B streptococci indicates a high degree of V region restriction in the L chains of antibodies elicited by hyperimmunization with streptococci.
D000596:Amino Acids; D000818:Animals; D000907:Antibodies, Bacterial; D005591:Chemical Fractionation; D055598:Chemical Phenomena; D002621:Chemistry; D002852:Chromatography, Ion Exchange; D007106:Immune Sera; D007147:Immunoglobulin Light Chains; D007525:Isoelectric Focusing; D010455:Peptides; D011135:Polysaccharides, Bacterial / Q000276:immunology*; D011817:Rabbits; D013292:Streptococcus agalactiae / Q000276:immunology*; D014357:Trypsin / Q000494:pharmacology
D000596:Amino Acids; D000907:Antibodies, Bacterial; D007106:Immune Sera; D007147:Immunoglobulin Light Chains; D010455:Peptides; D011135:Polysaccharides, Bacterial; D014357:Trypsin