Synthesis and characterization of membrane immunoglobulin, Ia, and H-2 molecules of thy 1+ AKR/J lymphomas.
Three AKR lymphomas displaying B cell and T cell characteristics have been described. Because of the proclivity of normal AKR/J mice to develop T cell lymphomas, and the rarity of lymphomas with dual characteristics, the B cell markers of these tumors were studied more intensively. Fluorescence data with class-specific anti-immunoglobulin reagents demonstrated that the tumor cells stained only with class-specific anti-IgM reagents. Because of the possibility that the surface Ig was passively acquired and of reports that certain anti-mu-chain sera react with "IgT", chemical characterization of the immunoglobulin molecules was performed. Using 3H-leucine internal labeling, we showed that all three tumor lines synthesized the immunoglobulin found on their surface, and that the immunoglobulin had the chemical and immunologic characteristics most typical of monomeric surface IgM, and was composed of mu-chains and light chains. The Ia antigens found on these cells were also examined. These antigens were also synthesized by the cells and were present in the same molecular form and in the same approximate quantity as Ia antigens on normal spleen cells.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology; D002462:Cell Membrane / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D006183:H-2 Antigens / Q000302:isolation & purification*; D005779:Immunodiffusion; D007075:Immunoglobulin M / Q000302:isolation & purification*; D007519:Isoantigens / Q000302:isolation & purification*; D008223:Lymphoma / Q000276:immunology*; D051379:Mice; D008806:Mice, Inbred AKR; D009368:Neoplasm Transplantation; D009374:Neoplasms, Experimental / Q000276:immunology; D011947:Receptors, Antigen, B-Cell / Q000302:isolation & purification*; D013601:T-Lymphocytes / Q000276:immunology
D006183:H-2 Antigens; D007075:Immunoglobulin M; D007519:Isoantigens; D011947:Receptors, Antigen, B-Cell