Insulin-mediated Na+ transport in the toad urinary bladder.
The characteristics of insulin-induced Na+ transport in the toad urinary bladder were determined and compared to those of aldosterone. Bladders were mounted in modified Ussing chambers, and standard short-circuit current techniques were employed to measure transepithelial Na+ transport. Insulin added to the serosal medium is much more effective than insulin added to the mucosal medium. Serosal insulin concentrations from 10(1) to 10(3) muU/ml increase both the initial rate and the final level of Na+ transport achieved, whereas concentrations from 10(3) to 10(5) muU/ml increase only the initial rate of Na+ transport. Insulin-induced Na+ transport probably does not require glucose. Both insulin- and aldosterone-induced Na+ transport are directly proportional to serosal (but not mucosal) K+ concentration over the physiologic range (2.0-7.0 meq/liter). However, cycloheximide abolishes aldosterone- but not insulin-induced Na+ transport. In addition, insulin stimulates Na+ transport after a maximal response to aldosterone, and aldosterone stimulates Na+ transport after a maximal response to insulin. Thus, although they have several similar characteristics, insulin and aldosterone have at least partially independent mechanisms of action on Na+ transport in the toad urinary bladder.
D000450:Aldosterone / Q000494:pharmacology; D000818:Animals; D001692:Biological Transport / Q000187:drug effects; D002024:Bufo marinus; D003513:Cycloheximide / Q000494:pharmacology; D004347:Drug Interactions; D004594:Electrophysiology; D005260:Female; D007328:Insulin / Q000494:pharmacology*; D008954:Models, Biological; D011188:Potassium / Q000378:metabolism; D014176:Protein Biosynthesis; D012964:Sodium / Q000378:metabolism*; D013997:Time Factors; D001743:Urinary Bladder / Q000378:metabolism* / Q000502:physiology
D007328:Insulin; D000450:Aldosterone; D003513:Cycloheximide; D012964:Sodium; D011188:Potassium