Two plant-type ferredoxins from a blue-green alga, Nostoc verrucosum.
Two plant-type ferredoxins were isolated and purified from a blue-green alga, Nostoc verrucosum. They were separable by chromatography on a DEAE-cellulose column. The slow-moving band was designated ferredoxin I (Fd I) and the fast-moving band was ferredoxin II (Fd II). The ratio of the yield of ferredoxins I and II was about 1 : 0.84. Both ferredoxins had absorption spectra similar to those of plant-type ferredoxins. Two atoms of non-heme iron and two of labile sulfur were found per mol of both ferredoxin I and ferredoxin II. Their molecular weights were identical and estimated to be about 18 000 by a gel filtration method. The biochemical activities of these Nostoc ferredoxins were studied: the NADP photoreduction activity on one hand and the NADP-cytochrome c reductase activity on the other.
D002736:Chloroplasts / Q000378:metabolism; D000458:Cyanobacteria / Q000378:metabolism*; D005288:Ferredoxins / Q000302:isolation & purification / Q000378:metabolism; D006418:Heme / Q000032:analysis; D007501:Iron / Q000032:analysis; D008970:Molecular Weight; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D009251:NADPH-Ferrihemoprotein Reductase / Q000378:metabolism; D010944:Plants; D013053:Spectrophotometry; D013056:Spectrophotometry, Ultraviolet
D005288:Ferredoxins; D006418:Heme; D007501:Iron; D009247:NADH, NADPH Oxidoreductases; D009251:NADPH-Ferrihemoprotein Reductase