[Nickel content of human lymphoblastoid cells cultured in a nickel sulfate-enriched medium].
Nickel is titrated by atomic absorption in nuclear and non-nuclear fractions of cells cultured in media supplemented by nickel sulphate. The living cell percentages are estimated in the same culture conditions. We try to connect the nickel presence in cellular fractions and the living cell percentages. These used cells seem to have mortality probability when nuclear fraction has 55 x 10(-6) of nickel and non-nuclear fraction has 33 x 10(-6) of nickel. We discuss the nickel stimulating effect for the start of DNA synthesis and the nickel penetration into cells.
D002467:Cell Nucleus / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media; D004247:DNA / Q000096:biosynthesis; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism*; D009532:Nickel / Q000378:metabolism* / Q000494:pharmacology; D013054:Spectrophotometry, Atomic; D013347:Subcellular Fractions / Q000378:metabolism
D003470:Culture Media; D009532:Nickel; D004247:DNA
Dosages du nickel dans des cellules lymphoblastoïdes humaines cultivées en milieux enrichis en sulfate de nickel. Remarques sur les modes d'action du nickel.